LOCUS       DAOQNP010000001        83487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_70_length_83487_cov_9.989189, whole genome shotgun sequence.
ACCESSION   DAOQNP010000001 DAOQNP010000000
VERSION     DAOQNP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 83487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 83487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83487
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_83487_cov_9.989189"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKY09_00005"
     CDS             <1..777
                     /locus_tag="PKY09_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB11259.1"
                     /translation="QMEYPMMINDNPTTTRFDGITLTTHEVFHQIFPFLMATNQTKYA
                     FMDEGWATLGEWYISPLIDTTIVDDYGMDRINSDLGKDWDAPMITNSTELTKSLFINS
                     YPKPALTFLYLWEMLGDSLFKKSLQHYIITWMDKSPTPWDFFFCMNQSCGMNLNWFWK
                     AWYFEQGYPDLAILDAKRLNYSYTVTVKSIGNKPVPVHLTATFADGTKKKFTRTIDIW
                     KHRKITEVIIDTQQAITKLELGDVYDADIDKSNNVFVPIE"
     gene            complement(808..2313)
                     /locus_tag="PKY09_00010"
     CDS             complement(808..2313)
                     /locus_tag="PKY09_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11260.1"
                     /translation="MSKKALIISTILVLMYNLHYGQQGINNNWLMGYSSWGGSPFGQT
                     RMNFFNGAPTISYDSLEMDFNHTHANISDAIGNMMFYTNGYYLADATNDTMQNGSGLS
                     PGAYANAFSDGFGIPQGALIIPKPNSSNLYYLFHNSADGYSQPIPNSISYNFYVTTID
                     MNVNGGLGTVLSKNVSLLTDSMNPGKIAATKHANGRDWWVMIHRVNTNKFYKFLVTPN
                     VILGPYSQNIGVIRGDGAGQAWFSPDGKKYAYYYVNDGLDIFDFDRCTATLSNAVHVN
                     MPFENGYNVGMAISPGSNALYVSNTQHVYQFDLTAANIAASQLTVATYDSFVSVFPGF
                     PNGFATLFGLSALAPDGKIYITTGNSTIHMHTIDNPDVIGLGCNVNQHSVQLQSVYFN
                     TLPNHPNYFLGCDTTCTTCLVGVPSLTPEGVMLRASPNPNNGNFTLQFNVQSTAGELE
                     IYDVMGKMVMRNYVAPWSQYKRVDITQLKKGIYFCKLKWKHTEQSLKVIKE"
     gene            complement(2364..2639)
                     /locus_tag="PKY09_00015"
     CDS             complement(2364..2639)
                     /locus_tag="PKY09_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11261.1"
                     /translation="MRLKKEKDGVLAAIYPNPANESATLTYSVPKESKVEFTMHTTTN
                     QQILQKSLRGGESKYTFSTTDLKPAVYFYELRNNGDLIAHGKLIIIR"
     gene            complement(2646..4151)
                     /locus_tag="PKY09_00020"
     CDS             complement(2646..4151)
                     /locus_tag="PKY09_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11262.1"
                     /translation="MSKKNQLLLILLLLVNGKITAQQGINNIWLMGYQSAAGYPGGQT
                     LIDFYSGLPILNYDSLEMDFNHTHANISDATGNIIFYTNGYYLADATNDTMQNGSGLS
                     PGAYANAFSDGFGIPQGALIIPKPNSPNLYYLFHNSADGYSQPIPNSISYNFYVTTID
                     MNGNGGLGAVLSKNVSLLTDSMNPGKIAATKHANGRDWWVMIHRVNTNKFYKFLVTPT
                     VILGPYSQNIGVVRADDAGQAWFSPDGKKYAYYYVNGGLDIFDFDRCAATLSNAVHIS
                     LPFENGYNVGMAFSPNSKILYVSNVFHVYQYDLSVTNIAASQLIVATYDSFLSVVPGF
                     PGYPTVFGLASLAPDGKIYLTTGNSTIHMHTVDNPDSIGIGCNVNQHSLQVQSFYFNT
                     LPNHPNYFLGCDTTCTTCLVGTPHLNPPRGGGLTALPNPSNGNFTLQFAVQKISGELE
                     IFDVMGNLVMRDYVAPWSQYKRVNIEGLAKGIYLCKLKWGGVEGSVKVIKE"
     gene            4864..4935
                     /locus_tag="PKY09_00025"
     tRNA            4864..4935
                     /locus_tag="PKY09_00025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4897..4899,aa:Arg,seq:cct)
     gene            5022..5264
                     /locus_tag="PKY09_00030"
     CDS             5022..5264
                     /locus_tag="PKY09_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB11263.1"
                     /translation="MSYYVYVLRNSVTKKLYKGQTQNLERRLKEHSQSKTKTTSHHAK
                     AWELVYHEIFETRELAVSREKYFKSAAGRRFLDKKV"
     gene            5427..5651
                     /locus_tag="PKY09_00035"
     CDS             5427..5651
                     /locus_tag="PKY09_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNB11264.1"
                     /translation="MLRNSVTKKLYKGQTQNLERRLQEHLQSKTKTTSHHAKAWELVY
                     HEIFETRELAVSREKYFKSAAGRRFLDKKV"
     gene            6028..6441
                     /locus_tag="PKY09_00040"
     CDS             6028..6441
                     /locus_tag="PKY09_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNB11265.1"
                     /translation="MQMQFFREQLKSKGLKVTPQRVAIYEAVAELHNHPTAENIIEYI
                     KTNHPNISVGTVYKVLDSLVENNLLRKVKNEKDIMRYDAIMSHHHHLYCSETDRIEDF
                     EDPQLDQFITEYFKKKKIKNFKVQDIKLQITGTFK"
     gene            6463..>7916
                     /gene="katG"
                     /locus_tag="PKY09_00045"
     CDS             6463..>7916
                     /gene="katG"
                     /locus_tag="PKY09_00045"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNB11266.1"
                     /translation="MDNTKSGKCPFHHGANTEASTGVMNWWPKALNLDILHQHDTKTN
                     PNGEKFNYAEEFKKLDLEALKADLKKLMTESQEWWPADHGSYSGMFARTAWHLAGTYR
                     KADGRGGANTGNQRFAPLNSWPDNVNTDKGRRLLWPIKKKYGNKISWGDLIVLAGNMA
                     YEVAGLKTFGFGGGREDIWHPEKDIYWGSEKQWLAPTGSEGTRYSGERDLENPLAAVM
                     MGLIYVNPEGVDGKPDPLKTAQDIRVTFSRMAMSDEETVALTAGGHTVGKAHGNGKAE
                     VLGGVPEAAEIQHQGLGWFNPDGSGNKDKTMASGIEGAWTTDPTKWDNGFFDLLLNYD
                     WALTKSPAGAWQYEPTNMPEEKKPVDAFNPNVRRNPMMTDADMALKMDPEFRKISERF
                     YKDFNYFSETFARAWFKLTHRDLGPKSRYLGADVPKEDLIWQDPIPAVNYTLSDAEAD
                     DIKSKILNSGLSRTELINTAWDSARTYRGSDFRGG"
     assembly_gap    7917..8016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8017..8436
                     /locus_tag="PKY09_00050"
     CDS             <8017..8436
                     /locus_tag="PKY09_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNB11267.1"
                     /translation="YAVSSEELLLDRTQLMGLTAPEMVVLVGGMRVLGTNFGGSKHGV
                     FTDREGVLTNDFFVNLTDMKYEWKPVSENLFNIVDRKTGATKYTATRVDLVFGSNSIL
                     RSYAEVYAQDDNKEKFVKDFVKAWVKVMNADRYDLKK"
     gene            complement(8518..11115)
                     /locus_tag="PKY09_00055"
     CDS             complement(8518..11115)
                     /locus_tag="PKY09_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNB11268.1"
                     /translation="MKHILLFLAIIPFFGIAQITDNFSDGDFTANPAWSGDNSQFIIN
                     ASQQLQLNSTGTATSYLALTTSLPMLDNTEWQFYIKLNFAPSSSNYARVYLVSDQQNL
                     KGVLNGYYVQFGETGSNDAIEIFKQTGTTSVSVARGTNGFIATASTISVKITRDNTGL
                     WSIYADAAAGTNFTLQSSGTDATINSGSYFGVSCTYTSSNATGFFFDNFSVPFIADVT
                     PPTLTSAIALSNTALDIKFSEPVDLNTAQTTANYAVQGLGTPSTAARDINDFSLVHLT
                     FGTPFTDGTAYTLVVNNVSDLNSNVIAAGSTIGFTWNAPVTAQPFDVVINEIYFEPVV
                     SAPLPNAEFVELYNRSNKLLSLNGWTLSDGSTSVASIGAVTLQPGAYIIICKNSETTS
                     FIPYGNTTGTSSFPSLNNDVGDDLKLTDNNGTLIDRVIFSDDNYNDDSKKTGGFTIER
                     VDADFTCNSRYNWHASNDAQGGTPGKVNSVKGTYSDTTPPQAVDAYPLTTTLIRITFS
                     ENPDNVIASQTSSYTIDNGIGQPASVVLSDNHAFLTLNNALQTGIVYHVNLNSSIADC
                     PGNKITGNLSLKIGIAETAVAGDLLINEILFNPKTGGNDFVELYNNSSKIIDLKNIKV
                     ATTDDNNLITTNYSVSDGGYLLFPQEYIALTGDVASLQSFYPDAAANNLLKASLPGFN
                     DDMGVCVITDFALNRLDQLSYNKRWHFPLIDDQNGVSLERIWFNKSTQDSTNWHSAAE
                     AVGYATPGYKNSQSLNEQLQENDFVITPEVFSPDNDGYNDVITFNINMNEPGYVLNAK
                     IYNEAGQLVRNLVKSKLLAPEDSFMWDGITDKNDRAAIGIYIVYAEAFNTGGKTKKYK
                     KAFVVASKL"
     gene            11233..12222
                     /locus_tag="PKY09_00060"
     CDS             11233..12222
                     /locus_tag="PKY09_00060"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNB11269.1"
                     /translation="MKVAVVGATGLVGKTMLRILEERKFPVSELIPVATANSAGKKIT
                     FCNKEYTVVTCEEAVALKPDIALFSAGGSTSLEWAPQFAQVGTTVIDNSSAWRMNEKC
                     PLVVPEINADVLTKGDKIIANPNCSTIQMVMVLNPLHKKYKLKRVVVSTYQSVSGTGK
                     KAVDQLMNERKGISGAMAYPYQIDMNVLPHVDSFLDNGYTKEEMKMVNETRKIMRSDE
                     IKVTATTVRVPVVGGHSEAINAEFDNQFELSDVKKLLSHTTGVTIIDDPSQLQYPMPT
                     FAEGKDDVFVGRIRRDESQPQTLNLWVVSDNLRKGAATNAIQIAEYLLKQNLI"
     gene            complement(12270..12950)
                     /locus_tag="PKY09_00065"
     CDS             complement(12270..12950)
                     /locus_tag="PKY09_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNB11270.1"
                     /translation="MGIYLIVIVFMLVGWLVSAQLKRKFEQYSHETLGTGLSGQEIAE
                     KMLRDNGINDVKVISVEGQLTDHYNPLNKTVNLSQPVYYGRNAAAAAVAAHECGHAVQ
                     HARAYTMLQVRSALVPVVSFASKWVQWVLLGGVLLINVFPSLLLAGIVLFAMTVLFSF
                     ITLPVEFDASKRALVWLNDSRMVTSSEHAKAKDALWWAAMTYVVAALGALATLMYYVL
                     IYLGGRRN"
     gene            complement(13074..14165)
                     /locus_tag="PKY09_00070"
     CDS             complement(13074..14165)
                     /locus_tag="PKY09_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11271.1"
                     /translation="MSYSQTKAMLAIAKASFRSITRSPSAVVFTLVFPLTFILVFGFL
                     SGTGLKVKLFLTPSSDHQNPIYEATKNIDVINWVEGDSAVASKKLQQGKIDGMLVITK
                     NVTEGKTDYTAKLISSTAAPEGGALSKNILDNLISKINLYDAHVAHPLAQLKSEEIAG
                     REYKKIDFILPGQLGFALLSTGVFGTAFVFFSLRQTLVIKRFFATPVKRIYIVLGETL
                     SRMVFSLLGACFIIGVGRFAFGFTLINGISTFLTMLLICAFGLLVFMGFGFVVSGIAK
                     SESTIPPIANIITMPQFLLSGTFFPIDVFPKWLQPFCKALPLTYLNDALRKVAFEGAG
                     LIDVVSQLLVLLLWGVIIYIIAAKTFRWE"
     gene            14266..14817
                     /locus_tag="PKY09_00075"
     CDS             14266..14817
                     /locus_tag="PKY09_00075"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HNB11272.1"
                     /translation="MNHTFSLFQSMERDKIILSFKGDITQDLLASVYQIMESRMDQDK
                     TDSKRKKRFYHILVECLQNVYHHMEDFRNVTELDEISDGSAIFIIAKAKGEGYRIITG
                     NYIKKEESEDLKSKLEKINSMNTEELRAYYLETLSTTELSQKGGAGLGMIDIARKSGH
                     KIVYSFDGVTDTVNFFTLEVTIN"
     gene            14828..15211
                     /locus_tag="PKY09_00080"
     CDS             14828..15211
                     /locus_tag="PKY09_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HNB11273.1"
                     /translation="MDKLIIEPGIKTPHIIFDAANGSLEIKGKSIPENSIEFYKPVYD
                     WLDTYATQPKDQTLLTVQLEYFNTSSSKCLLDLFRKLENIHSSGKGKVNVKWLYEEDD
                     EDIMEAGDDYQTIVKLPFSIEKISG"
     gene            complement(15212..17053)
                     /locus_tag="PKY09_00085"
     CDS             complement(15212..17053)
                     /locus_tag="PKY09_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB11274.1"
                     /translation="MKSILFLMLIPFAMFADKPGDERGVNIKDAHSYSNPAEIVVQHL
                     DLNLKADFSTKKLVGFVDLQVKNLTHADTLRLDTYNLKIESVKVNDAPVKFILKPADE
                     ILGSELAIPVTASTKVVTINYETSPDARALQWLSPEQTAGKIYPFLFTQSEAILARSW
                     IPLQDTPGIKFTWTATIHCPKELMALMSCPQNPKQIGNGNYSFKMPLPVPSYLMALSI
                     GDLAYKAYDNRCGIYAEPVMLGKAFHEFSDLPAMIASAEQLYGKYPWIQYDVLVLPPS
                     FPFGGMENPCVTFATPTIITGDRSLVSLIAHELAHSWSGNLVTNATWNDFWLNEGFTV
                     YFESRIMEKIYGKDYADMLNLLSQNELKHTVAVMGDNNPDTKLFLDLKGRDPDDGMGD
                     IAYEKGRFFLMALEEAAGRDVWDAFLKKYFEEHRFQTMTTEKFVKYLDANLLQENPLI
                     KQKVNVDEWVYGTGLPKSLPVIKSRYFDTVEKAAAAFIKTSAIPDTTRWTTHHYLHFL
                     RTVAPEMTYEKAVALDNHFHFTQIQNSEIAFDWFMIAIACHYKPAYQALENFLNTVGR
                     RKFIAPLYRKAAADTTLKPWIEKVYQTARPGYHAVAVKTIDEIFEGK"
     gene            complement(17064..17510)
                     /locus_tag="PKY09_00090"
     CDS             complement(17064..17510)
                     /locus_tag="PKY09_00090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB11275.1"
                     /translation="MKISWKFKPFNDLTAHELHDFLQIRQQVFIVEQNCPYSDIDKTD
                     KLSFHLMALANQQTVGYARIIPPGVLYDEVSIGRIVTHQQLRRTGLGKLVVEKALDEC
                     RSLFPNSPIKIMAQSYLCKFYESFGFTVISEEFMEDNIPHIYMFRT"
     gene            17650..18678
                     /locus_tag="PKY09_00095"
     CDS             17650..18678
                     /locus_tag="PKY09_00095"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNB11276.1"
                     /translation="MSWPQLIVTDSSRSGKLPFAPFKRTQEESAQQPKADKIIKKVVR
                     QNLPIKTDSINVTATVVDTIKPFANDSVQAANDSSVADMSRLFRFTDEENDELNNSDQ
                     QFISSSAYFRQHQLEVKSLSPQLFNKTVPDWFTIVIFFLIVGITTVKVFYQNIFRQLV
                     EAVYSFAVTNQVVRDENLLVQRASVLLNVIFYCSSGLFFYWISVRYNWNHPILNEGLL
                     RFLFFAFVTAVFFTVKMLLLKLLGTIFNLDKPVAVFIFSIFLTNNLLGVLLVLITSFM
                     AYLSGVSLINYVYLVLGILSLAFVFLLYRAFLISRTVQGYSLYYLILYFCTLEIVPIV
                     LIIKLASE"
     gene            18702..19457
                     /locus_tag="PKY09_00100"
     CDS             18702..19457
                     /locus_tag="PKY09_00100"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNB11277.1"
                     /translation="MAGNSKVKTILVTQPKPETEKNPYFDLAKKFNVKIDFRPFIQIE
                     GIPAKDFRKDRVNVAEFQAVILTSRQAADHYFRMASEMRMTNFDEVKFFCVSESTAFY
                     LQKYITYRKRKVFYGNNSINDLTDVLKKHKKEKFLLPCSEVMNPDIINTLKDGDLNFI
                     TAAMYRTVSSDLSDVNIESFDMIVFFSPSGVQSLLKNFPKYKQGNTRIATFGLSTSQT
                     AKDAKLKVEVEAPVPQAPSMTMAIEQYLKKANK"
     gene            complement(19463..20641)
                     /locus_tag="PKY09_00105"
     CDS             complement(19463..20641)
                     /locus_tag="PKY09_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11278.1"
                     /translation="MSSRKIEIFKTSDCIGKERLTKYVQGKMTDAEKHAIEKHLTDCE
                     LCSDAAEGLALLTTMKPLDETIRQVSDKYSPSAKQTPSINRWYYAAASVAILIATGWI
                     ISNYSFKQENTAEHISTPAAQQKLNTETPPSNSDAKLNESTIQPQPPPPPAEEKMQQA
                     SKNIMAAQNAMVAKASTEDKAFAATEPVQNNEAVAEPPSVDVAAPIATESASAGAVAP
                     VKSATKNIEGLKVYDYSGEYKREKNITKEDNGIPARYESRKDMEKSKTLNNMQSRTIA
                     YQDFLSEALADYNNKKYASAINKLQLLLSDNHSDVNALFYTAMSYSESQQYDKALHFL
                     DRLDAQSNNTFNQESAWHRSLLLLQKGEMDKAKELLQKIISAKGFYATAAQQKLSEIK
                     "
     gene            complement(20628..21224)
                     /locus_tag="PKY09_00110"
     CDS             complement(20628..21224)
                     /locus_tag="PKY09_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB11279.1"
                     /translation="MWLIRSKDKNALSDEELVLQYRQQGDKQAVGVLFNRYSHLVFGV
                     CMKYLKDVDDSKDAVLQIFEKLYTDLLKHDVQKFSYWVHRVAQNFCLMQLRSKQAAKN
                     RADIYNRDEIAATTDFVTENTDQVYEKEKELVMLEEAIKTLNEEQRICIELFFLKEKC
                     YNEITDLTGFDFKQVKSFIQNGKRNLRIYMEKNNHVKS"
     gene            complement(21307..21585)
                     /locus_tag="PKY09_00115"
     CDS             complement(21307..21585)
                     /locus_tag="PKY09_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11280.1"
                     /translation="MELTIIAKEDLSDYHFVQHEVLSNEIDRARRKILLDGAMALGND
                     YKQKVRIVFETTEGTCAVETTVWAVTNDHVELKAGKDIPIHVIQEVVL"
     gene            complement(21652..22683)
                     /locus_tag="PKY09_00120"
     CDS             complement(21652..22683)
                     /locus_tag="PKY09_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11281.1"
                     /translation="MQKSFLILWCFCFTLSLKAQQIELRTIQLPVQVSIRALQAVNDS
                     VVWFAANKGVFGFTKDGGNNWHIDSAAVDGKYPEFRSIAALNDSTVLLLSIDSPAYLL
                     RTNNYGASWQSVYSNHSKGVFFDSMTFKDSLQGIAISDPIDGHFMIIKTTDGGNNWQE
                     LKVTSPAMKGEGCFAASNTNIQWLQNSVIFATGGSTSRLFISENNGAFLPKNTPMISG
                     STMTGIFTMHFENKLNGFIGGGNYLQSDTTTTLYQTNDGGKTWQPIKLPDGAFVSCIK
                     TIKTKNDNYLIVTGHNGTYMVDSQHHIKDIKNADGQSLTFYTISITPSQKQIWMAGKS
                     GKMAVLKFY"
     gene            22904..24112
                     /gene="dinB"
                     /locus_tag="PKY09_00125"
     CDS             22904..24112
                     /gene="dinB"
                     /locus_tag="PKY09_00125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNB11282.1"
                     /translation="MSEQLNRVIAHMDLDCFFVSVSRLLHPELNNKPVIVGGGERGVV
                     AACSYETRKFGVHSAMPIKQAKRLCPEAIIVRGDYDAYSNHSDMVNEIICESVPLYER
                     SSIDEFYLDLTGMDRFFGCYKHATELRQRIMRETGLPISFGMSANKTVSKIATDEAKP
                     NNQMQVNCGEEKFFLAPLSVRKIPMVGQKTYTLLRSMGVEKVKTVQEMPVELMERVLG
                     ENGTTIWKKANGIDTTPVEPYHEQKSMSTEETFEQDTIDVQRLQHILIAMTEKLCFRL
                     RSEHKVTSCVAVKIRYSNFDTHTMQCRIPYTGCDHIIIKHVKDLFGKLYNRRLLIRLV
                     GVKFSHLVGGGHQINLFEDSEEIINLYQAMDNLRLRFGEDKIQRAAAMNFSLRGFNPF
                     NGLSKKPESK"
     gene            24571..25623
                     /locus_tag="PKY09_00130"
     CDS             24571..25623
                     /locus_tag="PKY09_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11283.1"
                     /translation="MIRRWLLSLVFFLLLLRGNAQVANDSCLSATNLGLLPIGSVLSV
                     PCLSGAPEIFIADSTTMAVANYPYPSIPNGCNGYVSTVASPAKDRWYKFTVNCDFTFR
                     VENSDTLHLSFWLGDSCSNLLPIECFTIPANTTFIQALSGLGGHWIYLQISGQDNINA
                     NHYNICFSTSIPMCAPSFNLGAFTPVLCFDYNLQVINSTSASINDGGIIINITEGNPP
                     YTCSWSDGATGFIRDSLQIGHYTFTIVDGIGCELTDSVTVGIITIQKDIIENDLHEIK
                     ILFNNLNHTLLLKSNSSSIIGREINLSIFDIGGQKVFNNKTKANIPFEFYCKNGIYLF
                     QISVQNCVINKKIYIN"
     gene            complement(25875..26852)
                     /locus_tag="PKY09_00135"
     CDS             complement(25875..26852)
                     /locus_tag="PKY09_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNB11284.1"
                     /translation="MLISVYVPHHAAIEVITPAYRLFNTANDILIASGKRPKFKVEYV
                     GLQKSIKANDGEYSIRIDRHISNVNDSDLIIIPALYGDVKHSLKKNSAAIPWLQKMHR
                     QGSEVASLCIGAFLLAASGLVDGKKVSTHWAYFNEFNDLFSNTVVVDGAIITEACGVY
                     SSGGANSLWNLLFYLLEKYSDRHTAILASKYFAIDLDRNSQASFAIFNGHKNHTDIEI
                     LKAQSYIEKHTDVRITVDQLADFTKMSRRTFERRFLKSTNHSVLEYIQRVKIETAKRK
                     FESSRKNITEVMFDVGYTDTKSFRDLFKKHTGVTPLEYRNKYSKISFNG"
     gene            26994..27443
                     /locus_tag="PKY09_00140"
     CDS             26994..27443
                     /locus_tag="PKY09_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB11285.1"
                     /translation="MQTNNVNLHRVFASPIEKVFRAFSDADAYSTWLPPYGFIAKTHQ
                     YDFKKGGSYKMSFVNFTTGFIHSFGGEFLEINTNELIKITDRFDDPNLPGEMITTIQF
                     KKVSCGTEVNISQTGIPEAIPSEMCYLGWQDSLDKLKRLVEPNIPDA"
     gene            27509..27913
                     /locus_tag="PKY09_00145"
     CDS             27509..27913
                     /locus_tag="PKY09_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HNB11286.1"
                     /translation="MLKQVFINLPVKDLEKSMSFYEAIGFTNNPQFTDHTAACMVWSD
                     TIFVMLLTHAKFKQFTSKQIIDAKQSVGVSNALSFESIETMKTFAANAVKAGGREYAS
                     ASDYGFMQQYFIEDLDGHNWEPFYMDMSKFPG"
     gene            27939..28409
                     /locus_tag="PKY09_00150"
     CDS             27939..28409
                     /locus_tag="PKY09_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB11287.1"
                     /translation="MAKKKIAPCLWIEKDAKKAAKYYLSIFKDGKMLSYKAYKNSGTP
                     SGDFDIAYINILGMDVQILAAGPLFKFSEAVSFVIICKDQKEIDYYWKALTKNGGSEG
                     PCGWCKDKYGLSWQVTPEQYFKLENHKNKAKQEYAFKALMKMKKIIIADLVENK"
     gene            28600..28998
                     /locus_tag="PKY09_00155"
     CDS             28600..28998
                     /locus_tag="PKY09_00155"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB11288.1"
                     /translation="MQLCAYIHFNGNCKKAMTFYKKCLGGKLSFQTVSELPQSESLPS
                     YMQRSILTALLQTKHFQLAGTDLNNESVYLPDNSFSLLLQCDSESDIKRLYRQLSAGG
                     VKTQLLQKNFYGVVIGSVKDKFGINWVLQC"
     gene            29101..32106
                     /locus_tag="PKY09_00160"
     CDS             29101..32106
                     /locus_tag="PKY09_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNB11289.1"
                     /translation="MCVAAVIGLLQYVAMHLCCHSYFSLKYGTISPEDLLCLAAQKNI
                     RSLALTDINNTSGILDFFRLAQRYNVCPVAGIDFRNGVEQKFIGIAKNSEGFHELNKL
                     LSEYLFSGERFSDKTPALQQAWIVYPLLSAPQQLLENEFVGIKPTELFQLNFSQWKSK
                     LHKLVALAPVTFRNKTDFNSHRLLRAISYNTLLSKLPVAGQAPADEIMYSPEEMKYIY
                     KEYPQLIYNTEKLLESCHIDFEFGKNKNKKYFLGSAKRDHHKLMSLCYEGLSYRYPNT
                     DDTIIDRFEKEIQMIGELGFSSYFLINHDIVRYARSKNYFYVGRGSGANSMVAYLLRI
                     TDVDPIDLDLYFERFINPQRTSPPDFDIDFSWNERDDVIDYIFNRSTFGREHVALLAT
                     YSTFQRDAAIRELGKVFGLPKAEIDSFFESGNAKRDHISNLVLQYAQRIIDLPSHLSI
                     HAGGVLISEKPMTYYTALDNPPKGFPLTQFSMLEAEDLGLYKFDILSQRGLGKIKDAV
                     AIIEKNRQVKVDIHDIKRFKEDEAVRHNLEQANLMGCFYVESPAMRMLLKKLKAKTYL
                     DLVAASSIIRPGVAQSGMMREYILRFHDESRRTYIHPMMEELMRETFGVMVYQEDVIK
                     VAHYFAGLTLTEADMLRRGMSGKYRGRAEFEKVKDAFFKGCAQKGHAPDTTAEVWRQI
                     ESFAGYAFSKGHSASYAVESYQCMFLKTYFPLEYMVACINNGGGFYAAEFYVHEARLN
                     GGIILAPDVNHSAYETTISGKNVYLGFHLVGELETKTAENILNARTQEGPFTSLAGFM
                     QRVEISLEQLRLLIRINALRFTGRTKQQLLWDIHEMIGHEKKTCTARQLFEAEHQHFA
                     LPELHSSQTDDTWDEIEMLGFPLGSPFDLLMAEPLPCNEKNIITLASELPQHCGRTVA
                     IAGYFVTRKPTRTKHGQPMAFGTFLDHNGQWIDTTHFPNVVKQYPFRGKGCYRITGKV
                     VQEFGFYSIDVTSLSLLPYKIRFADAAGN"
     gene            32410..35427
                     /gene="secDF"
                     /locus_tag="PKY09_00165"
     CDS             32410..35427
                     /gene="secDF"
                     /locus_tag="PKY09_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNB11290.1"
                     /translation="MQSKGAIKFLAILITIICLFQLSFTVVTNRIESKAHQATNGNPE
                     AYKAYIDSISQLTAYNLGFKKYTYQECKEREINLGLDLKGGMNVTMEVSVTDLIRNMA
                     NNPNDPMLVKALDDAHQAQRTSQKGLVQLFGEAFERNNPGAKLAGLYSTMDLQSRVNF
                     NSTNAEVIKVIQSEADAAISRTYNILRTRIDKFGVTQPNIQQLGGGRILIELPGVKEP
                     DRVRKLLQGTAQLEFWETYDNAEVFPQLEAVNKKLAALETPVPTDSSAKSDTTVAAAG
                     DSLTAAADTGAMKKPALLSKLESDTAASKDTSSSAQFAKFSKENPLFALLTPAIFTNA
                     QNQSEYRKGPVVGYAAIKDTGKLREILERPEIKNILPRDLRLLWTAKPPTKESKFLEL
                     VAIKVKSRDGKAPMDGSVITDARQDFGQFNSSPEISMSMNPEGAKTWKRLTGENVGKS
                     IAIVLDNYVYSFPNVQGEIAGGRSSITGNFEISEAQDLANILKAGKLPAPARIVEEAI
                     VGPSLGEEAIQNSWMSFAGAFVVILLFMGFYYMRAGWVANIALFANIFFIMGVLASLG
                     AVLTLPGIAGIVLTIGLSVDANILIFERVREELKAGKGIRLAVSDGFKHAMPSILDSN
                     ITLLILGIVLYIFGSGPVQGFATTLVIGVLTSLFSAVLLSRLIFEGMLDKNKPISFGN
                     EFSNNIYKNIHFNFVDNRKKYYIISSIVIIAGVVSFAIKGLNYGVDFEGGRTYTIQMD
                     KSISTEDIRMALEPQLVEAPEVKSVGTTDRFKITTAYLIHDTASNVENVIVDKLYKGL
                     TPLYSAAPDFETFKTKNIISSQKVGPTIADDIKQSAVWAVLISCALMFVYIFIRFKKW
                     QYGLGAVVALFHDVLIVLSFFTLLSGVIPVALEIDQHFIAAILTVMGYTMTESVVVFD
                     RIREYLGHHHTADDKHVINEALNATLSRTINTTLTVFFVLIVIFIFGGEVIRGFIFAL
                     LIGRIIGTYSSLCISTPIVIDLGGNAHEGVAKK"
     gene            complement(35522..36754)
                     /locus_tag="PKY09_00170"
     CDS             complement(35522..36754)
                     /locus_tag="PKY09_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNB11291.1"
                     /translation="MNLIIKIASRYLIGKKSHNIVNLVSLVSVAGVFTGTMALIVVLS
                     VFNGFGNLVISLYDTFDPDIKITAIEGKSFNTESISLDKINAINGVQYAAFTLEENCL
                     ARYGEKQDIVTLKGMDNELMKKTGLNKAVITGLPILKSGERNFAIVGSGVAYALSVNM
                     DTGNEPLMLYLPDKNITSPGDFENALRQMNISIGGIFAIQQDFDSKYILAPMSFVQEF
                     IAAGGKASAIEIMMKPGVDETAIIQKIKEISGHEFTVKNRAQQHEFLYKILSSEKWAV
                     FLILSFIMVIGIFNILGTLTMLVIEKKKDIIILKNLGAGFAMVKKIFFMEGLLITLVG
                     VVGGLLAGGIICFLQQHFHLIKLGNADSFVVEAYPVQMQVTDFVSVFLVVFCIGALAS
                     YFIAAHLVKRQYAETGKV"
     gene            36897..38024
                     /locus_tag="PKY09_00175"
     CDS             36897..38024
                     /locus_tag="PKY09_00175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB11292.1"
                     /translation="MKKLHMVDLISQYQKIKPEVDKAISDTVTSSAYINGPEVKAFQA
                     ELEKYLNVGHVINCANGTDALQIAMMALNLQPGDEVITASFTYVATAEVIALLKLKPV
                     LVEVIPGTFSIDPVAIEKAITPKTKAIVPVHLFGQCADMEPIMQIAAKHKLYVIEDVA
                     QAIGADYTFSDGRKQKAGTIGTIGCTSFFPSKNLGCMGDGGALFTNDDTLAKKIRMIA
                     NHGQSVQYVHDEIGVNSRLDSIQAAILRVKLKHLDSYAAARQKAAAYYDKAFSGNAKI
                     KTPERSKNSTHVFHQYTLVVNGVNRDELRQYLAEKEIPAMIYYPIPLHMQKAYKDDRY
                     KEGNFPVTEKLCANVLSLPMHTELDEEQLEYITRAVLEFCK"
     gene            38055..39368
                     /locus_tag="PKY09_00180"
     CDS             38055..39368
                     /locus_tag="PKY09_00180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNB11293.1"
                     /translation="MNIGVVGTGYVGLVTGTCFAEMGNHVVCVDIDQAKVDSMKQGKI
                     PIYEPHLDVLFERNIRQNRLSFTTQLADAVKHSSIIFLALPTPPGEDGSADLSYVLGV
                     ADQLGKMLDGYKIIINKSTVPVGTAEAVRDAIAKNYKGEFDVISNPEFLREGFAVDDF
                     MKPDRVVIGTRSEKAKKIMGELYAPFLRQGNPIYFMDERSAELTKYAANSFLATKITF
                     MNEVANLCEKVGANVDAVRIGIGSDDRIGKRFLFAGIGYGGSCFPKDVQALEHTSGKY
                     GYDFSILQSVMSVNNKQKMLLAEKVLEYYGGNVKGKRFALWGLAFKPDTDDIREAPSL
                     YIIDMLIKAGATIRAFDPEAMSNVKKQIGDKIEFANDEYHALEGADALLIATEWSAFR
                     TPDFDKMQSTLKSKVIFDGRNLYEPQKMQEAGYYYNSIGRQTVKP"
     gene            39365..40336
                     /locus_tag="PKY09_00185"
     CDS             39365..40336
                     /locus_tag="PKY09_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB11294.1"
                     /translation="MTKRILITGAAGFLGSHLCDRFIQEGYQVVGMDNLITGDLKNIS
                     HLFKLREFDFYHHDVSKFVHVPGKIDYILHFASPASPIDYLKIPIQTLKVGSLGTHNL
                     LGFALAKKARILVASTSEVYGDPLVHPQNEDYWGNVNPIGPRGVYDEAKRFQEAITMA
                     YHTYHKLETRIVRIFNTYGPRMRLNDGRALPAFIGQALRGEDLTVFGDGSQSRSFCYV
                     DDLVEGIFRLLHCDYAKPVNIGNPVEITIKQFAEEIIKLTGTTQKIVYKPLPQDDPKQ
                     RQPDITRAKEILGWTPKVSREEGLKITYEYFKSLPPEELKRSAHRFA"
     gene            complement(40468..41427)
                     /locus_tag="PKY09_00190"
     CDS             complement(40468..41427)
                     /locus_tag="PKY09_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNB11295.1"
                     /translation="MSNTKANNIKLGFFVMAGLLVLVIALYMIGSKRSVFSKTIEISA
                     VFHNVNGLMPGHNVRYGGIDIGTVQKIIFESDSAITVKMIIEKKMARYIKKNAIASIG
                     TDGLMGNKLVNINSLPEQSAPIEAGDVLLSLRPVESDEMIRTLNKTNDNLAAITSDLK
                     GLVQRFNKNNNLITLISDSTVIENLRQSIQSINSATNNIDNFTKDINNITKDVQNGKG
                     VLGKLIADSISENQLTVLLTNLQQLSDSLIEATNNINQFSGLLVNGQGTVQALLTDTI
                     MKNDFNETLNQLKKSSFLLEEDLKALQKNFLFRKYFRQKEKKK"
     gene            complement(41424..42170)
                     /locus_tag="PKY09_00195"
     CDS             complement(41424..42170)
                     /locus_tag="PKY09_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB11296.1"
                     /translation="MDNTNIILKIEGLCKAFGSNVVLNNFNLELVKGENIVVLGKSGS
                     GKSVLIKCIVGLLKPDKGNIHVFGTNMLELNQESLDKIRTKIGFLFQNSALYDSMTVR
                     ENLEFPMRRHWISLTSTEVKDLVEESLDNVGLLHAINMMPSELSGGMRKRIGIARTLI
                     MKPQLILYDEPTTGLDPITSREIVQLMLDLQKKYHTSSVIISHDMSVAKLTANKIIVL
                     LDGRNYAEGTYDELKKSSDKNINQFFEFAS"
     gene            complement(42173..42943)
                     /locus_tag="PKY09_00200"
     CDS             complement(42173..42943)
                     /locus_tag="PKY09_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11297.1"
                     /translation="MKLPKGISDLLHETGQLSAFVIRFFKEGFKPRYEWEELFKQAFI
                     NGYKSLPLVGITAFIMGLVLTVQSRPTLAEFGATSWLPAMIAISIVREIGPVITALIC
                     AGKIGSSIGAELGSMKVTEQIDAMEVSGTNPFKFLVVTRVLSIAFMLPILVILADLIA
                     IYACYIGVNFKDVVSWKLFYNKVFNSLEYSDFIPALVKSFFFGLTIGLVGCFKGYNSQ
                     KGTEGVGHAANSAVVVASLIIFILDLIAVQITDLLGLN"
     gene            43088..>44028
                     /locus_tag="PKY09_00205"
     CDS             43088..>44028
                     /locus_tag="PKY09_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB11298.1"
                     /translation="MAEKDSIPKIQTDSHIEVKQVYTSCKPMNELPAKFPFTRGVQAD
                     MYRSKLWTMRQYAGFSTAEESNKRYHYLLNQGTTGLSVAFDLPTQIGYDSDHELSEGE
                     VGKSGVAIDSLRDMEVLFDGIQLQNITTSMTINSTAAVLLSMYIALAKKQGADLSKIS
                     GTIQNDILKEYAARGTYIYPPKPSMRIITDIFDFCSREVPKWNTISVSGYHIREAGST
                     AVQELAFTLADGKAYLKAAIEAGLDINIFAKRISFFFNAHNNFFEEIAKFRAARRMWA
                     AITKELGATDPKAMMLRFHTQTGGSTLTAQQPQNNIMR"
     assembly_gap    44029..44128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <44129..44320
                     /locus_tag="PKY09_00210"
     CDS             <44129..44320
                     /locus_tag="PKY09_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB11299.1"
                     /translation="QVQKLVQLRSERNNEQVGQALNNLETAAKDNTNLMPFIIQCVEH
                     YATLGEISDALRKVFGEYR"
     gene            44424..44792
                     /locus_tag="PKY09_00215"
     CDS             44424..44792
                     /locus_tag="PKY09_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11300.1"
                     /translation="MKRAILFILTSAFLCSIFCQSLIFAHYFFNRTYYATVLCENKSK
                     KEMHCNGHCRLAKELKQQEKSAEIPFTSFKEKNEIVQYFELEEPFSFSEFFTDQIFVV
                     FKPFLEPEGYFQTIFHPPAV"
     gene            44871..45833
                     /locus_tag="PKY09_00220"
     CDS             44871..45833
                     /locus_tag="PKY09_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNB11301.1"
                     /translation="MRKIVFILLALIPYYAQSCDFCNGYLGLNPHFKKNSVSIRNQSR
                     FFKGTEMSATELDGMGLSKKDFFETRVNYELFAQYYPTQKLQLILSVPYVFNRDGMSD
                     KAAHAMGVTHHNLNHESKEATNTYQGIGDVVLVSHYQVFNINSTEEDKISQRLMVGPG
                     IKLPTGICDVPEGSESHTFVHQPGTGSWDPMISMIYLAKYKRSGLMANLSYMVTTKNK
                     QNFRSGNKLNTNVTGFYQITSKRFNIYPNIGFYWEYAAKDIYLSEKQGNSGGNISYLH
                     NGIDIYYKRFALNAAFQLPILKTLNGNQAEISFKVLTGFSFTLN"
     gene            45861..46613
                     /locus_tag="PKY09_00225"
     CDS             45861..46613
                     /locus_tag="PKY09_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11302.1"
                     /translation="MKKNTTIIAVIAIAIIGFCISGCKKDKDNEPTERELSFHLHTLV
                     GNTIANYNDVFSDNTGRKFKIDDCRFYISNIVLIKDDGTDYPLTNVVLLANPNVQEYN
                     LGKVPVGKYKGFRFLMGLDSVTNHADPASYPSTNPLSYQNPTIHWGWNNGYIFMEFQG
                     KFDSTFAANGTPDADFFYHVGMDNLKRTIDFSASAFEVSSNSDYEIGILFDLKKVLNN
                     VDMRTEFGTHTMDNMPLAVKIANNWQGAYSIE"
     gene            46646..47410
                     /locus_tag="PKY09_00230"
     CDS             46646..47410
                     /locus_tag="PKY09_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11303.1"
                     /translation="MNILRFVFFAAVAGLIFSLSSCKKDDSSDNQIPNTDTIGELSFN
                     LKQYVGNNRIAYNQVYSDTSGRQFKITNLRYYISNIVLLKANGQELPLSGKVLLVDNE
                     TEQYSLGEIPVGDYSGLRFMVGIDSATNHLDPALYDASNPLSYQPVSMHWGWNFGYIF
                     MKVEGNVDTDTIPTGNLNGIISYDIGSDPMVRVINFNHIFSVNGNTNTINIKFNLLKM
                     LSGIDLATESITHTNPGRDLAERIANNWLISFTIEP"
     gene            47407..48417
                     /locus_tag="PKY09_00235"
     CDS             47407..48417
                     /locus_tag="PKY09_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNB11304.1"
                     /translation="MIKKQKSKFFIISIFVFWIIGFTACRKDDAIDTGNEVLELQVPL
                     GFPYPPIPEDNQPTKNRIALGKMLFFDPILSRDSSISCSSCHHADKKFTDGLAVAEGI
                     DGRKTLRNSMTILNAAYQPNMFWDGGSPTLETQVSAPIEAHEEMDFNVMKVVERLNNH
                     AVYPDLFRKAYNSGPSAFTLTRAIANFERSLFTGKSKYDEYQYEKKSDALSASEKRGM
                     DLFFGERAECFHCHGGFNFTDFSFKNNGLYEVYADSGRARITTLHDDVGEFKVPSLRN
                     VALTAPYMHDGSMITLEEVVEHYNSGGKPNFNKSFLIRPLNLTAQEKEDLVNFLKVLT
                     DQ"
     gene            48428..48841
                     /locus_tag="PKY09_00240"
     CDS             48428..48841
                     /locus_tag="PKY09_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11305.1"
                     /translation="MKNISKLIALSFLLFSIVSCEKDDGCDGGTGGKNSLKVYLKHHS
                     KIIPPQSNYPDTLFIKYGSSDLPGTALSDFDNYVVNSDTSVDFLTVPNLKCGDYYLYG
                     VGWDAAISQRVKGGVPVTIPDNDAEVTVNVPVTED"
     gene            complement(48853..50478)
                     /locus_tag="PKY09_00245"
     CDS             complement(48853..50478)
                     /locus_tag="PKY09_00245"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNB11306.1"
                     /translation="MRIALAQLNFHVGNFENNTTKIIEAIKQAEKSGAALIVFPELAI
                     TAYPPLDFLEFDDFINRCYNSIEKIAAHSQNIAVIVGCPSRNAVPEGKSLFNSAWFIH
                     QGKVQSVIHKALLPTYDVFDEYRYFESGRIFQCIELNGEKIALTICEDLWNVEDDPLY
                     IHSPMEELIKEKPSFIINIAASPFDYKHAHQRKAILQRNVNQYKIPLLYVNHVGAQTE
                     IIFDGGSLAYDAKGRLVHEGPYFKEEIIYLNTANTSPVDEKQTEKYELIYQALLMGIR
                     DYFSKMGFKKAILGLSGGVDSAIVTVLAADALGAENILPVMLPSAFSSGHSISDSEQL
                     CQNLNIKVDKMEINPLYETFLKTLKPLFGDKPFDVTEENIQARIRGTLLMALSNKFGP
                     ILLNTSNKSELAVGYGTLYGDMCGGLSVIGDLYKTEVYELCNYINRDKEIIPENILSK
                     APSAELRPGQKDSDSLPPYDILDKILFEYIELRKGPREIIKGGHDETMVNRILSMVNR
                     NEYKRKQLSPVLRISSKAFGLGRRMPIVGKYLS"
     gene            complement(50639..51229)
                     /locus_tag="PKY09_00250"
     CDS             complement(50639..51229)
                     /locus_tag="PKY09_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB11307.1"
                     /translation="MRAKVSLIAIAFVAAFQFVKAQDNKFNFGLKISPTVAWFKASDN
                     LDNHGSKIGFAYGLIADINFTNNYSFETGIDVTYRGGKLKYLDTYKMKTVLQYIELPL
                     TLKLKTNEIGYMTYFGKVGFTTGVNIRTDGDFKKSDINPLNLALVAGIGTQYSLGGKT
                     ALLLGITFNNGFLDVVKEKHSKATSNFLAVDLGVMF"
     gene            51262..52281
                     /locus_tag="PKY09_00255"
     CDS             51262..52281
                     /locus_tag="PKY09_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11308.1"
                     /translation="MKFFTVRHLFYFLLSTVLMVVFSCQNDPHGDKHAAEAITTHLKF
                     DEVLFSADSISPQHVVQWEQHYPYFFNLFVNRIIHLLPTDSAALALNLQKFVGDKDVK
                     DIHARVEKVFSSADKDKLFSEIEFFLSNYKNELGTQPVKHLVTFLSAFNYAVITADST
                     IGIGLDMYLGSDCEFYPSIGIPQYAYQRFSKDYILSDVIKGWYQSEHDIDAEKNDFLS
                     RMIYYGKQLYFTSVMVPQMQDSILTGYSKKQLEWCNNNSKQMWAFFIENKLFYSTREM
                     EYMKYIADGHATQGFPEGAPSRTAQWLGWKIVSAYMKNNSVKLKELLAEKDAQKILEK
                     SGYKP"
     gene            52312..52641
                     /gene="gldC"
                     /locus_tag="PKY09_00260"
     CDS             52312..52641
                     /gene="gldC"
                     /locus_tag="PKY09_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HNB11309.1"
                     /translation="MKKSEIKFTVSLDDNNKPVALHWNAEDSGMQGDKECKAMMLALF
                     DKKDQTTMRIDLWTQEMMVEEMQQFFFEMYMSMADTYQNATNDKDQAEKMRGFARQFG
                     KEVSLIK"
     gene            complement(52963..53367)
                     /locus_tag="PKY09_00265"
     CDS             complement(52963..53367)
                     /locus_tag="PKY09_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNB11310.1"
                     /translation="MNHFLLFETILYVKNQQESAVFYTKLFRQEPDLNVPGMTEFILA
                     PNCKLGLMPNYGIAKILSDKTPHPNLGNGIPRCELYYYAENIELEFDNAINAGAHLIS
                     PIIDRDWGDRACYFSDPDGHIIAFAQKITNKG"
     gene            complement(53460..53870)
                     /gene="arfB"
                     /locus_tag="PKY09_00270"
     CDS             complement(53460..53870)
                     /gene="arfB"
                     /locus_tag="PKY09_00270"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNB11311.1"
                     /translation="MDKIDFKCLLSEVVLTYSRSSGKGGQNVNKVSTKAELAFNIDSS
                     ACLDEEQKKIISEKLYRRINDEGFIKLQSSEARSQLGNKELVLKKFKTLIEKSLKKPV
                     KRIATSKPLQATESRLTQKRRTSEIKKLRQKKSD"
     gene            complement(53870..54652)
                     /locus_tag="PKY09_00275"
     CDS             complement(53870..54652)
                     /locus_tag="PKY09_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNB11312.1"
                     /translation="MSTLRVTTVQTKLHWESQVKNIKHFTTLLKSIKTGSTDLIVLPE
                     MFSTGFSMNANALAEKSDGPTMQWMQKMAETKNAVVCGSLIIEENGRFYNRLIWMTPG
                     GSHAHYDKRHLFTMAGEQKFYHPGNKKLILLYKGWRLSVFVCYDLRFPVWMRNQNEYD
                     CAIVVANWPEKRSFAWKQLLTARAIENQSFVVGVNRIGKDGNGITYSGDSVVLDPFGK
                     KISKTKSDTENIETIKLDFDVLENARHIFPVGNDADSFSIKL"
     gene            complement(54707..55870)
                     /locus_tag="PKY09_00280"
     CDS             complement(54707..55870)
                     /locus_tag="PKY09_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNB11313.1"
                     /translation="MSANQALIKSKLPKTGNSIFSVMSALANECNAINLSQGFPDFAI
                     SPELISLVTKYMKKGMNQYAPMPGIMPLREEIAAKIEARFSAKYNPETEITITAGGTQ
                     AIYTAIASVVREGDEVIIFDPAYDSYAPAVVLAGGEPVHAELKMPDFHIDWNEVKKLM
                     NQRTRMIMINTPHNPTGAILSVKDFQMLEKITAKTDIFVLSDEVYEHVIFDGYEHQSV
                     ARFPKLAERSFIVYSFGKTFHTTGWKMGYCLAPAKLSAEFRKAHQFMVFSANTPIQYA
                     YAEFMKDAKNYSGIEKFYQEKRDYFLKLIQGTAFKPLACSGSYFQLLDYSKLSREKDM
                     DYAVKLTKDYGVASIPVSAFYRNGTDHKLLRFCFAKSEETLEKAAERLHKVEK"
     gene            56132..57886
                     /locus_tag="PKY09_00285"
     CDS             56132..57886
                     /locus_tag="PKY09_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNB11314.1"
                     /translation="MKFPSINFSIYGKRTYLVLISVLIGALTTLCAAILKKGVMLMEH
                     IAEHLVFDSGYHQLFLILPATGILLTVTFWKLMKIKNERRGMTAVIYNISKQSSVVAK
                     HKLFSQMVSSIFTVGFGGSAGLEAPIASTGSAIGSTIASKLKFSTKERTLLLACGAAA
                     GISAIFNAPIAGVVFALELLLIDMPVPVVIPLLISSATAAFLSRYFNLGQPFVLITDT
                     WNSSHILYYLALAALAAILSIYCIKIYFFISSIFSKISNVFVKALIGGTLLGLLIFLF
                     PPLYGEGYDSVQLLLHSQSGEIFKNITFFEANALWLPILAALLMFFLKVVATSFTVFG
                     GGNGGMFGSSLFTGAMLGFTFSRVVNLMGWAELNEINFTVIGMASLLSGVLQTPLTAI
                     FLIAEITGGYALFVPLMITTSITYLATRYVEPHSVYTKQLALKGGLINLSKDKQVISK
                     MKLRDYLETDLETIKINATLGDLIKAIRKSKRNIFPVVDAMNRLQGIILLDDVKRVMF
                     DQSQYGTGVKELMQRPPLVLDIEEDMELVLEKFEEYEAWNFPVTEKGVYAGFVSKSKI
                     FTFYRQLLIQESDVPMHD"
     gene            complement(57946..58311)
                     /locus_tag="PKY09_00290"
     CDS             complement(57946..58311)
                     /locus_tag="PKY09_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11315.1"
                     /translation="MMNNSMIESTTGAEMVEAKKAAPKKKAATKKKAATKKKAATKKK
                     AAPKKKATKKKAATKKKATKKKAATKKKVVSAKKAAPKKKAAPKKKAATKKKAAPKKK
                     AAPKKKAATKKKAATKKKK"
     gene            complement(58376..59623)
                     /locus_tag="PKY09_00295"
     CDS             complement(58376..59623)
                     /locus_tag="PKY09_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB11316.1"
                     /translation="MTDLFDKISKNRGPLGMHAKESHGYFTFPKLEGEISNRMIFRGK
                     EKLVWSLNNYLGLANHPEVRKIDGEASVHYGLGTPMGARMMSGETDRHTLLEQQLAAF
                     EQKEDAILLNYGYQGMVSAIDCLVDRHDIIVYDSESHACIIDGVRLHMGKRFVYPHND
                     INNLVKQLERATKLAEETGGAILVITEGVFGMSGDQGKLKEIVALKSKFSFRLFVDDA
                     HGFGTMGKTGAGTGEEQGVQDGVDIYFGTFAKSMALIGGFIASDAQIIEYFKYNMRSQ
                     IFAKSLPMPLVEGAIKRLELLKNQPQLKNNLWKIVNALQNGLKQAGFDIGKTQSPVTP
                     VYLNGTIPEATKLIYDLRENYDIFCSMVVYPVVPKGIIMLRLIPTAVHTLEDVKYTIE
                     SFGKIKSKLLNGEYKAEKIVDVN"
     gene            complement(60203..60502)
                     /gene="raiA"
                     /locus_tag="PKY09_00300"
     CDS             complement(60203..60502)
                     /gene="raiA"
                     /locus_tag="PKY09_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNB11317.1"
                     /translation="MKIKVQSIHFDADKNLIRFVEEKVDKLKQFYEHIIDGEVFLRLD
                     KNVTQENKIAEIKISTPGKVLFASEQCATFEQAADKSVEALKRQITKHKEKQRGS"
     gene            complement(60617..61504)
                     /locus_tag="PKY09_00305"
     CDS             complement(60617..61504)
                     /locus_tag="PKY09_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNB11318.1"
                     /translation="MPYAKFLRFLQYEKRFSQHTLTAYGTDLKQFFDFMGQTYQAQKP
                     EEITHFFIRSWIVSLLDRKIDSRTVNRKISSLKTYFKFLMREKTISQNPMLKIVSPKT
                     AKKLPVIVDRQKMDQLLDGVAFKEGFEGIRDKLIINMFYQTGMRLAELIGLKDKNVDL
                     YNLTLKVLGKRNKERIIPITPEMKEAIVVYLNERNNVIDEKIKSEYFFIKNDGNKLYP
                     KFVYKVVNVMLSQVTTNKKRSPHVLRHTFATEMLNSGAQINAIKEILGHSSLAATQVY
                     THNTIDKLKNIYKQAHPKA"
     gene            complement(61600..61803)
                     /gene="rpsU"
                     /locus_tag="PKY09_00310"
     CDS             complement(61600..61803)
                     /gene="rpsU"
                     /locus_tag="PKY09_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNB11319.1"
                     /translation="MIIVQIKDNEPLDKALKKFKKKFEKTGVVKQLRSRQAYEKPSVA
                     RRTEVKKAIHRTKLQRLEAEGAM"
     gene            complement(61979..63253)
                     /gene="purD"
                     /locus_tag="PKY09_00315"
     CDS             complement(61979..63253)
                     /gene="purD"
                     /locus_tag="PKY09_00315"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNB11320.1"
                     /translation="MNILLLGSGGREHAFAQKLSESALLKHLYIMPGNAGTLQLGTNI
                     SGSVNDFDHIKKTVLEKKIDMVIVGPEEPLVNGIHDFFLGDSELKSVPVIGPERSGAR
                     LEGSKDFSKAFMLRNNIPTARYETFESSQLQQGYNFLNTLSAPYVLKADGLAAGKGVI
                     ICETETEAREQLHHLLINQPFGKAGNKVVIEEFLNGIELSVFVLTDGNNYCLLPEAKD
                     YKRIGDNDTGLNTGGMGSVSPVPFAEAAFMQKVEDKIIKPTINGLKHENISYTGFLFI
                     GLMNVNGNPFVIEYNCRMGDPETEAVFPRIKTDFIELMNSVAQKKLHEVELKTDDRTS
                     FTVMMVSGGYPETYEKGKIISGLEKVKGVNVFHAGTILQDDKVLTSGGRVLAVNALGK
                     NLAEAREKVYEQLAEITYEKAFYRKDIGRDLM"
     gene            63447..63899
                     /locus_tag="PKY09_00320"
     CDS             63447..63899
                     /locus_tag="PKY09_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNB11321.1"
                     /translation="MSSLFNKQDGEKIIERIATLQSGQKPNWGTMSVEQMLLHCQQPF
                     KVAFEELKLKRSLVAKLFGGIAKKSVMSNKPFKENLPTATEFKTNHLNPQFETEKKNL
                     IACIQKFQSAGPEGITQQPHPFFGKMTDKEWDTLQYKHLDHHLRQFGA"
     gene            63896..64456
                     /gene="lptC"
                     /locus_tag="PKY09_00325"
     CDS             63896..64456
                     /gene="lptC"
                     /locus_tag="PKY09_00325"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNB11322.1"
                     /translation="MKLRFIPVVFIISLLMMSCENDIEQVKLVTGNVVEPIESAKGLE
                     ILYSDSGKVKVRISAGEMNRYLQPKPITEMPKGVKIDFFDDNLKVTSTLTSDYALRDD
                     FDKNMEAKKNVEVVNVKGEKLMTEYLKWDEKTGLISSPEFVKIITADEIIYGNGFESN
                     QDFSRYKIFNIKGIINLKKNENTENT"
     gene            64434..64631
                     /locus_tag="PKY09_00330"
     CDS             64434..64631
                     /locus_tag="PKY09_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11323.1"
                     /translation="MKILRILEISWLIIAIGSLCAGIYKLISAGLNQAVFIFAISIIA
                     FVFFMIRRKQRISMEKQNASR"
     gene            64669..65952
                     /locus_tag="PKY09_00335"
     CDS             64669..65952
                     /locus_tag="PKY09_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNB11324.1"
                     /translation="MISDFLPAIVASLAFVAFYSGIEIAYLSSNKFKIELDSKQGKWS
                     GKILSYFSKSPSRFICTILVGLNISLVIYGTNMSGLLNPYLQKWLPQQLNSEIGRLII
                     ETLITTFFLLLAGEFLPKILFNINPNDTLNFFSGFIWLSYIILFPLVWLILKLAHFLL
                     RNVFKVDFIEEATGFGRVDLDNFIEEISENSGGKSEYNKEIQMFQNALDFDSLKVRQC
                     MIPRNEIISLSSKESVETLRKKFVETGLSKIMIYQDTKDNIVGFVHSYEMFKNPESIM
                     SVLLPVSIFPETLPARELLTHLTTNHRSVAIVVDEYGTTSGMVTIEDIIEEIFGEIDD
                     EHDIDVYLEQKISETEFLFAGRLEVDYLNQKYNLMLPESELFTTLAGLIFNHLEDIPN
                     VKEQFTIGNYKLTVEQVSGNKIEQVRLIILDNTET"
     gene            complement(65961..67862)
                     /locus_tag="PKY09_00340"
     CDS             complement(65961..67862)
                     /locus_tag="PKY09_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNB11325.1"
                     /translation="MSDILHHECGVAMIRLLKPLDYYHRKYGTPFYALNKLYLLMEKQ
                     HNRGQDGAGVANIKFDAEPGTRYISRYRSNDKHAIQDIFSRIYNKFKADVNGDESKLS
                     DTAWMKKHLAFSGEVWMGHLRYGTYGGNGIEACHPFLRQNNWMTRNLVVAGNFNMTNV
                     DELFENLIRIGQHPKEKADTVTVMENIGHYLDEENERLYYSFKEKGFSKQDISPLIAE
                     HLDVESILKQSSKKWDGGYVICGMMGHGDAFVLRDPAGIRPAYMYADNEVVVVASERP
                     AIQTAFNLSFSDITEIKPGHALVMKKNGNISMPCIKEPVERKSCSFERIYFSRGSDAE
                     IYQERKELGRLLCKSILPAVDNDLRNTVFSFIPNTAEVAFYGMMQGMEDYLREVKKKK
                     LKSLGKEISDEKLNEILSIKPRFEKIAIKDAKLRTFITNDDDRSDMVTHVYDITYGTI
                     RKGIDNLVVIDDSIVRGTTLKNSIIRMLDRLGPKKIIIVSSAPQIRYPDCYGIDMAKI
                     GDLCAFKAAIALLEENNMEHIIEETYMHCKQQLELGVNEMKNYVRDLYKPFSPEQLST
                     KITSMLKPEDCKADVEIIFQKIEDLHTACPYNKGDWYFTGNYPTPGGNKVVSKAFINY
                     MQGINTRAY"
     gene            complement(68023..69732)
                     /locus_tag="PKY09_00345"
     CDS             complement(68023..69732)
                     /locus_tag="PKY09_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11326.1"
                     /translation="MKLTFKQRLFLYISIAFALFALSVFLFEQTREKRIKSEAIEEKL
                     GAYADIVNESIAKKTPDQYILILDNLSTLFPANLRLTIVNRNGKVLYDNSILGWNKLE
                     NHLGRPEIANSIKNRTGTDIRHSLSTGKEYLYFAKNEGDYFVRVALPNDVQVQRFFKS
                     DNVFLYVTLLLFCILLILVNWIANSFGNSINELRNYAVNPKAKAALNFPKNEIGEIGK
                     EIAENYKQLRATKNKIELEREKLLQHIHSSQEGICFFTVKKNAELYNGLFIQYTHIIT
                     DENYSDPKVIFVDRAFEELQQFLNDDKDNFKEYKIQKQGKTFSVRAIIFEDKSFEVVI
                     NDITKQENVKILKQQMISNIAHELRTPITGVSGYLETVLEKNLDAKTQQYFIAQAFNQ
                     TKVLTELVQDMNLINKIDEAGYTFKKSPLDISFLLKKIQEEYALQLRDNNITLHLKLP
                     EQIIINANESLIKSIFKNLFDNAIRYAGKDISIFLTLLKEDKNYYYFSFADTGVGISD
                     EKHLPRIFERFYRVDEGRTRETGGSGLGLSIVKNAITLHGGTISAKTRKDSGLEFIFQ
                     LHK"
     gene            complement(69729..70412)
                     /locus_tag="PKY09_00350"
     CDS             complement(69729..70412)
                     /locus_tag="PKY09_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB11327.1"
                     /translation="MNENILIVDDDKSICEILEFNLKSEGYIVDTAYSAEEALTKLTN
                     QTQLILLDVMMEGMSGYKMLEKIRLDGSTIPVIFLTAKNTENDMLTGFSVGADDYISK
                     PFSIKEVSARIKAILKRFKTASTEQKETLAFKDLEINTKNKEAFIKGKPVNLTKTEFE
                     ILQKLAKNPNRIFSRQEIIEGVWGNNVFVTERTVDVHITRLRKKLEDYGNIISNRLGY
                     GYKFDLMKL"
     gene            complement(70455..71126)
                     /gene="phoU"
                     /locus_tag="PKY09_00355"
     CDS             complement(70455..71126)
                     /gene="phoU"
                     /locus_tag="PKY09_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNB11328.1"
                     /translation="MKHTEKEIQLLKDEVKLMWKLVISQLENAKKAMLNNDIALATKI
                     IRNENDVNTFELKVDNLCENFIALNNPVAIDLRLALSLIKISSSLERIGDFTDGIARF
                     ITDKECETLDPDLIERLNIEKMFDTLLGMLLDGFVAFETEQTKVSEKIISKDDQVDEI
                     YNNVFETLATHIKNNPAQTICMLKLLLLIRKLERIGDHNNNIVEEIVFFVDARVLKHS
                     GSNNF"
     gene            complement(71172..71918)
                     /gene="pstB"
                     /locus_tag="PKY09_00360"
     CDS             complement(71172..71918)
                     /gene="pstB"
                     /locus_tag="PKY09_00360"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNB11329.1"
                     /translation="MIEAKNVNFYYHTFHALKDINMQIEDNSVTALIGPSGCGKSTFL
                     RLFNRMNDEIDGAKLTGECLINNENIYHKSVQVDLLRKNVGMVFQKPNPFPKSILENV
                     AYGLRVNGINNKQLIEEKVKESLTAAALWNEVKDKLHKSAFELSGGQQQRLCIARALA
                     VSPSILLMDEPASALDPISTAKIEELIFELKQRYTIIIVTHNMQQAARVSDKTGFFMY
                     GELVEHSETKKMFLAPDKTETQNYITGRFG"
     gene            complement(71915..72769)
                     /gene="pstA"
                     /locus_tag="PKY09_00365"
     CDS             complement(71915..72769)
                     /gene="pstA"
                     /locus_tag="PKY09_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNB11330.1"
                     /translation="MIISKKTKEQIAFAIFRILGIITLGVLIWILGFICFNGISVMSW
                     DFIFSYPEEGMTSGGIFPAIVGTLYLVLGSIIIAFPLGVMSAIYTVEYAKNNWVTKLI
                     RIMTNNLASIPSIVFGLFGMTLFVNTLAFGDSIIAGSLTLSLLILPVVIRTTEEALKA
                     VPNTYRTGSMALGANKIQTIKRIILPTAFPNIITGLVLSIGRVSGETAPILFTVVAYF
                     LPKLPSSIFDQVMALPYHLYVLATSGTDIEKSRPVAYGTALVLIIIVLLMNTVAGVIK
                     NQFQKNKK"
     gene            complement(72766..73962)
                     /gene="pstC"
                     /locus_tag="PKY09_00370"
     CDS             complement(72766..73962)
                     /gene="pstC"
                     /locus_tag="PKY09_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNB11331.1"
                     /translation="MNKVLEKFVESLLKMSSAVTGITVLLIIIFLFKEGLGLFKSSTV
                     EKGYTLVVNTQNPVNRLTAVEIKEIFDSEIENWKDVGGSNDDIRIFRFDEIFTMYSDE
                     ELGEDYSLLPQKLGEIIQSDSTIIAFLPLKYVPKNMSGIKELKTDIISISDFFLGTTW
                     LPTATPSALFGILPLVVGTLLVSFIAIVIAIPFGVGVAIYLSEIASEKVRKVMKPAIE
                     LLAGIPSVVYGFFGLVVVAPIVQKVGGLTLGETAFTGSLILAIMALPTIITISEDALR
                     NTPKAMRDASYALGANRWQTIYKVVIPYASSGISAAIILGIGRAIGETMAVLMVTGNA
                     AVMPHSLFESVRTIPATIAAELGEAPGGGTHYQSLFLLGCVLFLITLIISIISEKISG
                     RKIIKE"
     gene            complement(73985..74797)
                     /locus_tag="PKY09_00375"
     CDS             complement(73985..74797)
                     /locus_tag="PKY09_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNB11332.1"
                     /translation="MKKSILTAIVIILTLSGSYAQKIKGSDTVLPLTQEEIEAYKKEK
                     PSASISVTGGGSGVGITALLNGTTDIAQSSRKIKFDEKQKLAEAGKAVKEVIIAYDAL
                     SIVVHPSNKVSQLTREQLEKIFTGKITNWKEVGGDDMKIIPYSRETSSGTYEFFKESV
                     LKNKNYMNGILSMPATGGIIQSISQTKGAIGYVGFAYVTDKVKALNVSYNNGKTFIEP
                     TIANAKNKSYPIVRPLYYYYTNLSAEGKSFIDFILTPKGQNVVSKVGYITIK"
     gene            complement(74876..>75496)
                     /locus_tag="PKY09_00380"
     CDS             complement(74876..>75496)
                     /locus_tag="PKY09_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11333.1"
                     /translation="INNSIKDIIKWGAGVSYYNGGVYQGKKYIYKNSGNGFIVDSSAN
                     NLGSFAKREYVGFNLYLTAYSAIGLTKITSEYLFGTQPGAKSNSKSPNAATLPTNDVY
                     MRQFNGGYVMLVQDFGTLPVSAIVKYDWYDPNTKSKGDEIGKNNTDAGDVALNTTGFG
                     LQWKINTNFRLTGYYELVQNEKCINLKGYETDKKDNAFTCRLQYKF"
     assembly_gap    75497..75596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<75597..75889)
                     /locus_tag="PKY09_00385"
     CDS             complement(<75597..75889)
                     /locus_tag="PKY09_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11334.1"
                     /translation="MKKLLLTVGYILTGTTLFAQHDTTMVQGKSISDLEQKIYEIEDA
                     LKDVRNLSVSGYIQTQFQYGEKDASLKVGSANENKEKSYNRVGIRRGRIKFAYT"
     gene            complement(76596..77216)
                     /locus_tag="PKY09_00390"
     CDS             complement(76596..77216)
                     /locus_tag="PKY09_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11335.1"
                     /translation="MKKLIIVLSVAATSVIIGCSKDDLTSPQALQNKNTTAATTDVSV
                     TDERLINSSTVLVPAPGQGYVKVFLFGAAKLSADKPETFVDINKRDVSQRTFDASFKA
                     MYVKFNERWIYVPGVIPNYGVEGKLTYRTSSTQAVDPDTPIQVLSWHRFTLTIRNLDN
                     TPYTGRLISLSNIKVLIIPAKSDPSGHDPDLRNYEATMTHFGLPLD"
     gene            complement(77503..78168)
                     /locus_tag="PKY09_00395"
     CDS             complement(77503..78168)
                     /locus_tag="PKY09_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB11336.1"
                     /translation="MELSNLKILIADDHKLFAEGLTYLLKQELSIESIDTVYNGKSAI
                     GKVLNTDYNIIIMDVNMPLLNGIETCAEIKRFRTQTKIVFVSMKADMLTVAQALKAGA
                     DAYLLKGNDHNDFIKAFKQIYNNEIFLSEQIKHYFQNDNPNQIRKNAIAYLDYSNSLI
                     SAREKEIIKLVCDGLTSDKIGEVLSISTRTVDTHRNNILNKLKLNNIASLVRFALENK
                     LVE"
     gene            complement(78178..79539)
                     /locus_tag="PKY09_00400"
     CDS             complement(78178..79539)
                     /locus_tag="PKY09_00400"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNB11337.1"
                     /translation="MDFLFYTHESKLYGLTIFVSIVAFQIVYVFVQWLIEHRRDYLFY
                     IAYLSFVIVFGLSQYDKYLNFYPFSEIAEKYYGYIKLSVPLFCILLYFRFMREFLNLK
                     HRRPDINAMVKKLEIFLFAYTLLSLFFIWLSLDIVVWQRIFNVVAIITITAGLFIIIN
                     FLKKSIPLDRFAMTGASLIAVGSIFDIVLNFLEFRGFDIPFDIHLPLMVCVIGELTTF
                     TLGLAYKTQLVENEKLFVEKKFLTEKSEKQQLQLDILKLRNNLSRELHDDIGAQLSLA
                     RMLMSKARVDGNERVVDQSMQLLEESINKLRSVTNELENSTLQNEGFQKATEKLLNTI
                     MEFQPIHCNFYTKGVEKRLHLDVEYHLFRITQELINNTLKYAHASIISIIVLGDRDKF
                     YFIYEDNGLGFDIKGNGRGKGLNNILQRVEELGAKTEFITQPGVGMRCEFKIDYEHSM
                     QKA"
     gene            79859..80359
                     /locus_tag="PKY09_00405"
     CDS             79859..80359
                     /locus_tag="PKY09_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNB11338.1"
                     /translation="MSKYNLHYAGLKEGIHEFDYLLNQDFFDMFEHPIVQGAHVLIHL
                     TVNKSATLVVLNIKAAGTVHVQCHRCLEEFEMSLNITKTIIVKTSGEDKEVDDDNIIL
                     ADNAHDIHLAQHFYDLVSLAIPIKVTHPDNGCNEETLRTLEKYMVKNENTNDPRWDIL
                     KNVSNN"
     gene            80379..80564
                     /gene="rpmF"
                     /locus_tag="PKY09_00410"
     CDS             80379..80564
                     /gene="rpmF"
                     /locus_tag="PKY09_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNB11339.1"
                     /translation="MPNPKHKHSKSRRDKRRTHYKAEAPTIAVCPTTGETHLYHRAYW
                     HEGKMYYKGKVVLEKAV"
     gene            80583..81548
                     /gene="plsX"
                     /locus_tag="PKY09_00415"
     CDS             80583..81548
                     /gene="plsX"
                     /locus_tag="PKY09_00415"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNB11340.1"
                     /translation="MRIGVDIMGGDYAPLETTLGAIAALNDLSPQEVHLVLIGDEKKI
                     TEIIDGQGVNPRLFTIVNATEVIEMSDSATRAFSQKPNSGMAIGFGLLKQKKIDAFAS
                     AGNSGAMLVGSMFSVKAINGIQRPAISTILPQEDGSTGILLDVGANADCKPEVLQQFG
                     LLGSLLAEHVYHIAAPRVALMNIGEEEEKGNLLSQQAHQLLKNTKEINFIGNVEGRDL
                     FNNKADVIICDGFTGNVMLKEAESLYTLLKKRGIKDEYFDRFDYENYGGTPVLGVNGN
                     VIIAHGISKSKAIKNMILLAKEVTEAHLAEKIATFFQNNVVNQTL"
     gene            81557..82552
                     /locus_tag="PKY09_00420"
     CDS             81557..82552
                     /locus_tag="PKY09_00420"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNB11341.1"
                     /translation="MEKIRAAITAVNAWVPDYVLTNKELETMVETNDEWIQTRTGIKE
                     RRILKDAGEGTSKIGVESVNGLLKKRGIQAEEIDLLICATTTPDLVFPATANIICDKV
                     GAKNAFGYDIQAACSGFLFALTTAAKFVESGQYKKVVVVGADKMSAIIDYSDRATCII
                     FGDGGGAVLLEPDTNGYGIYDAVLKSDGSGRHFLHQKAGGSVKPPTHATIDAKEHFVY
                     QEGQTVFKFAVKGMADVSAEIMERNNLSADDIAWLVPHQANKRIIDATANRMGIGMEK
                     VMLNIQKYGNTTNGTLPLCLWEWEKQLHKGDNLILAAFGGGFTWGAVYLKWAYNS"
     gene            complement(82706..83167)
                     /gene="tnpA"
                     /locus_tag="PKY09_00425"
     CDS             complement(82706..83167)
                     /gene="tnpA"
                     /locus_tag="PKY09_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNB11342.1"
                     /translation="MANTYTQIYLHIVFSVKGRLNLIQKDWKEELHKYICGIVNGKEQ
                     KVYAIGGVADHIHILVSIKPNIALSDLVRDIKASSSKWINEKGLVKEKFQWQEGFGAF
                     SYAQSQLDTVIAYINNQEQHHKKKTFRDEYLELLQKFDVGYDEKYLFEWIE"
BASE COUNT        25830 a        15299 c        15515 g        26543 t
ORIGIN      
        1 cagatggaat atccaatgat gattaatgac aatccaacaa caacacgatt tgatggaatt
       61 acattgacaa cacatgaagt ctttcatcag atttttcctt ttctgatggc taccaatcaa
      121 accaagtatg cttttatgga cgaagggtgg gcaacactgg gcgagtggta tatcagtccg
      181 ctaattgata ctaccatagt ggacgattac ggaatggata gaattaattc tgatttgggt
      241 aaagactggg atgcacccat gattaccaat tctactgagc taacgaaatc attattcatt
      301 aacagttatc caaagcctgc gctcacattt ctatatttat gggaaatgct aggcgattca
      361 ttattcaaaa aatcattgca gcattacatc attacctgga tggataaaag cccgacacca
      421 tgggatttct ttttctgtat gaaccaaagc tgtggcatga atttaaactg gttttggaag
      481 gcatggtatt ttgaacaagg ctaccccgac ctggctattt tagatgcaaa acgacttaat
      541 tacagctata cagttacagt aaaatctatt ggcaacaaac ctgtgcctgt gcatctcaca
      601 gcaacgtttg cagatggaac caaaaagaag tttacacgaa ctatagatat ttggaagcat
      661 agaaaaatca cagaagtgat tatagacaca caacaggcaa ttacaaaatt ggaattgggc
      721 gatgtttatg atgcagacat agataaaagt aataatgtat ttgttccgat agagtgaggg
      781 gagcgccctg catggggcat tggtaagcta ttcttttatc acctttaagc tttgttcggt
      841 atgtttccat ttgagtttac aaaaataaat tccttttttt agttgtgtta tatctactct
      901 cttatactga ctccacggtg caacataatt cctcatcacc atttttccca tcacatcata
      961 aatctccagt tctcccgctg tactttgcac attaaactgc aatgtaaaat ttccattgtt
     1021 gggattaggc gaagcacgta gcattactcc ttccggggtt aggctgggca cgcccaccaa
     1081 acacgttgta caagtggtat cacatcccaa aaaataattt gggtgattgg gcagtgtatt
     1141 aaaataaaca gattgcaatt gcacactgtg ttggtttaca ttgcatccca aacctattac
     1201 atcagggtta tctatggtgt gcatgtgtat agtgctattg cctgtagtaa tgtagatttt
     1261 tccatccggt gcaagggctg acaatccaaa caatgtagca aaaccattag gaaacccggg
     1321 gaaaaccgat acaaatgaat cgtatgtggc aaccgtcaac tgtgatgctg caatatttgc
     1381 tgctgttaaa tcaaactgat atacatgttg cgtattagag acgtacaagg cgttagaccc
     1441 cggtgaaatt gccataccca cattatatcc gttctcaaat ggcatattaa catgcactgc
     1501 attacttagt gttgcagtgc atctgtcaaa atcaaaaata tccaagccgt catttacata
     1561 gtaataagca tatttttttc cgtcaggtga gaaccatgcc tgccccgcac catcacctct
     1621 tataacgcct atattttgtg agtaaggccc taaaataaca ttaggtgtca ccaaaaactt
     1681 ataaaactta tttgtattaa ccctgtgtat cataacccac caatccctgc catttgcatg
     1741 ttttgtagca gctatttttc cggggttcat agaatccgtt aataaagaaa catttttact
     1801 taaaacagtc cctaatcctc cgtttacgtt catgtcaatg gttgttacat aaaagttata
     1861 ggaaatactg ttgggtatag gctgcgaata gccatcagca gaattatgaa aaagataata
     1921 aagattagag ctattaggct ttggaattat caatgcacct tgtggtattc caaatccatc
     1981 actgaaagca tttgcataag cacccgggct taaaccactt ccattttgca tagtatcgtt
     2041 agtggcatcc gccaaataat aaccattggt ataaaacatc atgttaccta tagcatctga
     2101 aatattcgca tgagtatggt taaaatccat ttctaaacta tcgtatgaaa tagtaggggc
     2161 accattaaaa aagttcatcc ttgtttgccc aaacggtgaa ccaccccaac ttgaatagcc
     2221 catcagccaa ttattgttaa tgccttgttg cccataatgc aagttataca ttaacactaa
     2281 aatcgttgaa ataataagtg cttttttact cataatttag aaattaaaaa ggaggaaccc
     2341 caaagttcct cctttacaaa aatttaacgg ataattatca atttaccatg cgcaattaag
     2401 tctccattgt ttcgcagttc ataaaaataa actgcgggtt ttaaatcagt cgtgctaaat
     2461 gtgtactttg attctccccc tcgcagcgac ttttgtaaaa tttgttggtt cgttgtagta
     2521 tgcatagtaa actctacctt actctcttta ggcacactgt aagttaacgt agccgattca
     2581 ttggcagggt ttgggtaaat ggctgcaagc acaccatcct tttctttttt tagccgcaca
     2641 atttcctatt ctttaataac ctttacactc ccttcaacac ctccccattt taatttacac
     2701 aggtaaattc cctttgccaa accctcaatg ttaactctct tatactggct ccacggtgca
     2761 acataatccc gcatcaccaa atttcccatc acatcaaata tttccaactc tccgcttatc
     2821 ttttgtacag caaactgcaa agtaaaattc ccgttgcttg gattaggcaa tgccgtaagt
     2881 cctcccccac ggggaggatt taggtggggc gtgcccacca aacacgttgt gcaggtagta
     2941 tcacatccca aaaaataatt ggggtgattg gggagtgtgt taaaataaaa ggattgtacc
     3001 tgtaagctat gttggtttac gttacaacca atacctattg aatcagggtt gtcaactgtg
     3061 tgcatgtgta tggtgctatt tcctgtcgtt aagtaaattt taccatcagg tgccaatgat
     3121 gccaacccaa aaactgttgg ataacctggg aaaccaggca ccacagacaa aaaagaatca
     3181 taagttgcta ctatcaattg tgatgctgca atattagtaa cacttaaatc atattgataa
     3241 acgtgaaaca catttgatac atatagtatc tttgaattag gcgagaatgc catccctaca
     3301 ttataaccat tctcaaatgg caaactaatg tgtacagcat tacttaatgt tgcagcgcat
     3361 ctgtcaaaat caaaaatatc caagcctccg ttcacataat aataagcata tttttttccg
     3421 tcaggagaga accatgcttg tcccgcatca tccgctctca caacgcctat attctgtgag
     3481 taaggcccaa gaataacagt aggtgttacc aaaaacttat aaaatttatt tgtattaacc
     3541 ctgtgaatca taacccacca gtctcttcca ttggcatgtt tggtagcagc tattttgccc
     3601 ggattcatag agtcagttaa taaagaaaca tttttactta aaacagctcc caaccctcca
     3661 tttccattca tgtcaattgt tgttacgtaa aagttatagg aaatactgtt aggtatgggc
     3721 tgtgagtaac catcagcaga attatgaaaa agataataga ggttaggact attgggtttt
     3781 ggtattatta aagctccttg tggtattcca aatccatcac tgaaagcatt tgcataagca
     3841 ccaggactta aaccacttcc attttgcatt gtatcattgg ttgcatctgc caaataataa
     3901 ccattagtat aaaaaatgat gttgccggtt gcatctgaaa tattcgcatg tgtatggtta
     3961 aaatccatct ctaaactatc ataatttaaa ataggaagac cgctgtaaaa atctatcaaa
     4021 gtttgtcctc cgggataacc ggcagcactc tgatagccca taagccagat attatttatt
     4081 ccttgttgtg cagtgatttt tccattcacc aatagtaaca agattaaaag aagttggttt
     4141 tttttactca taatatagga gttaaaaaaa gaaggaactg aaaaaaagca gttccttctt
     4201 tttaagaatt aacgtacaat aaccaatttg ccatgtgcaa ttaaagcacc attgcttctc
     4261 aactcataaa aataaactgc gggttttaaa tcagtcgtgc taaacgtgta ctttgattct
     4321 ccccctcgta attttattgt tatctgccgc gcaaaaactg ataagttaat taaacagcat
     4381 attaaagtta ttgctgctac cgttaagtat tttttcatgt tttctattgt gatattaggt
     4441 aaattttatg tatataatta caagcatact agcaacataa accaaacatc ctgtcaaaaa
     4501 gcaggagaag aattacacac ttggcggagt atctatgttt tccatagtcc tcttaattta
     4561 aagtttacac caaaaaaaat aaaaattcgt tacataaaga atattagttg aaaaataatt
     4621 tttatggggc gcctctccca gttgcaaatt gcaacagaaa agagcgacta attgctgcaa
     4681 tctttttatc tgccaacaca tcatgctctc caacataaaa aggatttctc aacggatacc
     4741 cgcccgtacc gaacggtacg ttcgggcggg gaatagaagt ttcctgagaa taaaataaag
     4801 caacaattga atttatgtta aagtaaagtg agcaaatact ttatttttgt gctttgaaaa
     4861 aatggtcccg tagttcaacg gaaagaatag aagtttccta aacttttgat ggcagttcga
     4921 ttctgcccgg gactacaaaa tataaaaaag aaggcggttc cctgcccgac tgaagtcatt
     4981 caggcgggga ttcctgctgg gacaatcaaa aaaaataccg catgtcatac tatgtttatg
     5041 tgctaaggaa cagcgtgaca aaaaagctat acaagggtca gacacaaaat ttggagagac
     5101 gtcttaaaga gcattcacag tcaaagacca aaaccacaag ccatcatgca aaggcatggg
     5161 agttggtata tcatgagatt tttgaaacca gagaacttgc agtatcaaga gaaaaatact
     5221 tcaagtcagc ggcaggaaga aggtttttag ataagaaagt ttgaattgaa ggttcaacgg
     5281 atacccgccc gtaccgaacg gtacgttcgg gcggggaaca gaagtttcct aaacttttga
     5341 tggcagttcc ctgcccgact gaagtcgttc aggcggggat tcctgccggg actactaaat
     5401 aaaatcattc atgccatact gtatatgtgc taaggaacag cgtgacaaag aagctataca
     5461 agggtcagac acaaaatttg gagagacgtc ttcaagaaca tttacaatca aagaccaaaa
     5521 ctacaagcca tcatgcaaag gcatgggagt tggtatatca tgagattttt gaaaccagag
     5581 aacttgcagt atcaagagaa aaatacttca agtcagcggc aggaagaagg tttttagata
     5641 agaaagtttg aattgaaggt tcaacggata cccgcccgta ccgaacggta cgttcgggcg
     5701 gggaacagaa gtttcctaaa cttttgatgg cagttccctg cccgactgaa gtcgttcagg
     5761 cggggattcc tgccgggact actaaataaa atcattcatg ccatactgta tatgtgctaa
     5821 ggaacagcgt gacaaagaag ctatacaagg gtcagacaca aaatttggag agacgccttc
     5881 aagaacattc acagtcaaag atcaaaacta caagccatgc aagtgaagga ggcgaatatg
     5941 cagaagaacg gaaatgaata ttacttaaag acgaataatt tctaaacgag aattattatc
     6001 tttgcggaat tgttataagt cgtaagtatg cagatgcaat ttttcagaga gcagctaaaa
     6061 tccaaggggt tgaaagttac accacagcgt gtggctattt atgaagccgt tgcagaattg
     6121 cacaaccatc ccactgcaga aaatattatt gagtacatta aaaccaatca tccgaatatc
     6181 tctgtaggta cagtgtataa agttctcgac tcattggtag aaaacaatct gttacgaaaa
     6241 gtaaaaaatg aaaaagatat tatgaggtat gatgcaatca tgtcacatca ccatcatttg
     6301 tattgttcag aaaccgatcg tatagaagat tttgaagacc cgcagttaga tcagttcatc
     6361 actgaatatt ttaagaaaaa gaaaattaaa aacttcaaag tacaggacat taaattacag
     6421 attacaggaa catttaaata aaaaaaataa aacacacata ttatggacaa cacaaaaagc
     6481 ggcaaatgcc cgtttcatca tggagccaac actgaagcaa gtacaggtgt tatgaactgg
     6541 tggcccaaag cattgaactt agatatcctg catcagcatg atacaaagac aaatcctaat
     6601 ggcgaaaaat ttaattacgc tgaagaattt aaaaaattag atttagaagc gttaaaagct
     6661 gacctgaaaa aattgatgac tgaaagtcag gagtggtggc ctgcagatca tggaagctat
     6721 tcgggtatgt ttgcacgtac tgcctggcac ttagcaggta cttacagaaa agctgatgga
     6781 cgtggtggtg ctaacaccgg taatcagcgt tttgctcctc tcaattcatg gcctgataat
     6841 gtgaacaccg acaaaggaag aagattactt tggccaatta agaaaaaata tggtaacaaa
     6901 atttcctggg gtgatttgat tgttttagca ggtaacatgg cttatgaagt tgcaggatta
     6961 aaaacatttg gcttcggtgg cggtagagaa gatatatggc atcctgaaaa agatatttac
     7021 tggggatcag aaaaacaatg gcttgctcca acaggcagtg aaggcacacg ttattcaggt
     7081 gaaagagatt tagaaaatcc attagcagcg gtaatgatgg gattgattta tgttaaccct
     7141 gaaggtgttg atggaaaacc cgacccgcta aaaacagcgc aagacattcg tgttactttt
     7201 tcaagaatgg caatgagtga tgaagagacc gtagcactta ctgccggtgg acatactgtg
     7261 ggcaaagcac atggtaatgg caaagcagaa gtgttaggtg gtgttcctga agctgcagag
     7321 attcaacatc agggattagg ctggtttaat cctgatggct caggcaataa agataaaacc
     7381 atggccagtg gtattgaagg tgcatggaca accgatccaa cgaaatggga taatggattt
     7441 ttcgatttgt tattgaatta cgattgggca ttgacaaaaa gccctgcagg tgcatggcag
     7501 tatgagccaa ccaatatgcc ggaagaaaag aaacctgttg atgcattcaa tcctaatgtg
     7561 cgcagaaatc ctatgatgac agatgctgat atggcattga aaatggatcc tgaattccga
     7621 aaaatatcag agagatttta taaggacttc aattactttt ctgaaacttt tgcacgagca
     7681 tggtttaaac ttacccatag agatttggga cctaagtcac gttacctcgg tgccgatgtg
     7741 cctaaagaag atttgatttg gcaagaccct attcctgcag ttaattacac attgtcagat
     7801 gctgaagcag atgatataaa atctaaaatt ttaaactcag gtttaagcag gacagagttg
     7861 attaatactg catgggattc tgcaagaaca tatcgtgggt cagatttcag aggtggnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatg cagtttcttc agaggaatta
     8041 ttattagaca gaacacagtt gatgggactc actgctccag aaatggttgt tcttgttggt
     8101 ggtatgagag ttttgggaac taactttggt ggaagcaaac atggggtgtt tacagacaga
     8161 gaaggtgttc taactaatga cttttttgta aaccttactg atatgaaata cgagtggaag
     8221 cccgtttctg aaaatctgtt caacattgtt gatagaaaaa caggagcaac aaaatacacc
     8281 gctacaagag tagatttggt tttcggctcc aattcaattt taagatcata cgcagaagtg
     8341 tatgcacagg atgataacaa agaaaaattt gtgaaagatt ttgtgaaagc ctgggttaag
     8401 gtgatgaatg ctgacagata tgatttgaaa aaataaaatt gtttagtact tcaaaaatgc
     8461 gaacagcctc ttttcagagg ctgttcgcat tttatatttc ttgtttaaat tgagtcatta
     8521 aagttttgaa gctaccacaa aagctttttt atactttttg gtttttcctc cggtattaaa
     8581 tgcttcggca taaacaatat aaataccaat agcagctcta tcatttttgt cggtaatgcc
     8641 atcccacata aaactatctt ccggtgccaa tagttttgat ttaactaagt tgcgaaccaa
     8701 ttgtcctgcc tcattataaa tttttgcatt cagcacataa cccggttcat tcatgttgat
     8761 gttaaaagtt atcacatcat tgtatccatc attatcagga gaaaaaactt ccggtgttat
     8821 cacaaaatca ttttcctgta attgttcgtt taaactttgt gaatttttat aacccggtgt
     8881 tgcataacct acagcttctg ctgccgaatg ccagttggta gaatcctgtg tacttttatt
     8941 aaaccatatc ctttccaatg aaacaccatt ctgatcgtca atgagtggaa aatgccatcg
     9001 cttattgtag ctaagctgat caagtctgtt caatgcaaaa tcagtaataa cacaaacgcc
     9061 catgtcgtca ttaaaacccg ggagcgatgc ttttaataaa ttattggctg cagcatcggg
     9121 ataaaaactt tgcagagatg caacatcacc ggtaagtgct atatattcct gcggaaacaa
     9181 aagatatccg ccatcactga cactgtaatt tgttgttatc agattgttgt cgtcagttgt
     9241 tgcaaccttt atgtttttaa ggtcaattat ttttgaagag ttgttatata gctccacaaa
     9301 gtcattgccg cctgtttttg ggttgaacaa tatttcgttt atcagcaaat cacctgcaac
     9361 agcagtttct gcaataccaa tttttaaact aagatttcct gttattttat ttccgggaca
     9421 gtcagcaata gatgagttta aattaacgtg ataaactatt cctgtttgta atgcattgtt
     9481 tagtgttaaa aaagcatgat tgtctgaaag tactaccgat gcaggctgtc caatgccatt
     9541 gtcaatagta taacttgatg tttgtgaagc tatgacattg tcaggattct cactaaaagt
     9601 tattctaatc aatgttgtgg taagaggata agcatcaact gcctgaggag gtgtggtgtc
     9661 gctgtaagta cctttaacac tgtttacttt tcccggtgtt ccgccttgcg catcatttga
     9721 tgcatgccag ttatatcttg aattgcaagt aaaatcagca tcaactcttt caatggtgaa
     9781 tcctcctgtt tttttgctgt catcgttgta gttatcatca ctgaaaatta ctctgtctat
     9841 caatgtgcca ttgttatcag tcaatttcag gtcatcgcca acatcattat tcaacgaagg
     9901 gaacgaactt gttcctgttg tattaccata agggataaaa gatgttgtct cagaattttt
     9961 acagataatg atatatgcac ccggctgtaa tgttactgcg ccaatgcttg ctacggaggt
    10021 tgatccatca gataatgtcc agccattgag tgaaagtaat ttgttgcttc tgttgtacag
    10081 ctctacaaac tctgcattag gtagtggtgc cgatacaaca ggttcaaaat atatttcgtt
    10141 aattacaaca tcaaaaggct gtgctgttac aggagcattc catgtaaaac ctatggtgct
    10201 tcccgcagca attacattac tattaagatc agatacatta tttacaacaa gcgtgtatgc
    10261 agtgccgtct gtaaaaggag tgccaaatgt cagatgtact aaagaaaaat catttatatc
    10321 gcgtgctgcc gtggacggag tacccaaacc ctgcactgca taattcgctg tagtttgtgc
    10381 cgtattcagg tcaacaggtt cagaaaattt tatatctaaa gcagtattcg ataatgctat
    10441 tgccgaagtt aatgttggtg gtgtgacatc tgcaataaat ggaacagaga aattgtcaaa
    10501 gaaaaaacct gtggcattgg atgaagtgta agtacagctc acaccaaaat aacttcctga
    10561 gttaattgtt gcatcagtac ccgaagactg taaagtaaaa tttgttcctg ccgcagcatc
    10621 ggcataaata ctccaaagac ctgtgttgtc tcttgttatt tttacactga tggttgatgc
    10681 ggttgcaata aatccgttag tgccacgtgc tactgataca gatgttgtac ctgtttgctt
    10741 aaaaatttcg attgcatcgt tgctgcccgt ttcgccaaac tgaacataat agccattgag
    10801 tactcctttt aagttttgtt gatcgctcac caaataaact cttgcataat tgcttgaaga
    10861 gggtgcaaaa ttgagcttaa tataaaattg ccattcagta ttatccagca tgggcaggct
    10921 tgttgtcaat gccagataag aagttgctgt accggtgctg ttgagttgca gttgctgcga
    10981 ggcattgata ataaactgac tgttgtcgcc actccaggca ggattagcag taaaatctcc
    11041 atcagaaaag ttgtctgtaa tttgtgcaat accaaaaaat ggaattattg caagaaaaag
    11101 tagtatatgc ttcatgccaa taaaaaatat aaggcagtga agataagcaa accccattat
    11161 ttaaaataat taaattgaaa caggtttaag taatgtgttt tttattcatt tttgaggtag
    11221 gaataaaata ttatgaaagt agcagtagta ggagcaacag gtctcgttgg aaaaaccatg
    11281 ttgcgtattt tggaagagag aaaatttccg gtgtcggaat taatacctgt tgcaacggca
    11341 aattctgccg gaaaaaaaat aactttttgt aataaggaat atacggtggt aacttgtgaa
    11401 gaggcagttg ccctaaaacc tgatattgca ttattctctg ccggagggtc aacatcttta
    11461 gaatgggcac cacaatttgc acaggttgga actactgtta ttgataactc atcggcatgg
    11521 cgtatgaacg aaaaatgccc tttggtagtc cctgaaataa atgctgacgt attaactaaa
    11581 ggagataaaa ttattgccaa cccaaactgt tctaccattc agatggtgat ggtacttaat
    11641 ccgctgcata aaaaatacaa actcaaacga gttgttgtat caacatatca gtcagtttcc
    11701 ggaacaggaa aaaaagctgt tgatcaactg atgaatgaac gcaaagggat ttcaggtgct
    11761 atggcctatc catatcaaat tgacatgaac gtgttgccac atgttgattc ctttttagat
    11821 aatggatata cgaaggaaga aatgaaaatg gttaatgaaa caagaaagat aatgcgatcg
    11881 gatgaaataa aagttacagc aacaactgtg cgcgtgccgg tggttggagg tcatagcgaa
    11941 gcgatcaatg cagagtttga caatcagttt gaattgagtg atgtaaaaaa acttttatcc
    12001 catacaacag gtgtgaccat cattgatgac ccttcacaac ttcaatatcc gatgcccaca
    12061 tttgctgaag gaaaagatga tgtttttgta ggaagaatca gacgcgatga atcgcaacca
    12121 caaacattaa acctttgggt tgtatctgac aatctcagaa aaggcgcagc taccaatgct
    12181 attcagattg cagagtattt gctaaaacag aatttgatat aaataaaaag ccaccccact
    12241 aaggatggct tctttcagaa aattattttt tagttgcgtc ttccgcctaa gtaaatcaaa
    12301 acataataca tcagcgtagc caaggcacct aatgcagcaa caacataggt catggcagcc
    12361 caccataaag catcttttgc ttttgcatgt tcactgcttg ttaccatgcg gctatcgtta
    12421 agccaaacca atgctctttt acttgcatca aactccacag gaagtgtaat aaaactaaac
    12481 aacactgtca tggcaaacaa cacaatacct gcaagtagca gcgatggaaa aacattaatg
    12541 agtaatacac cgccaagcaa tacccactgc acccattttg aagcaaaact tactacaggc
    12601 accaatgctg aacgaacctg aagcatggtg taggctctgg catgttgcac agcatgacca
    12661 cactcatgtg ctgcaacggc agcagcagca gcattacgtc cataatatac aggctgactc
    12721 agattaacag ttttgttaag cggattgtaa tggtctgtca actgaccttc aacagaaata
    12781 acttttacgt catttatgcc gttgtcacga agcatttttt ctgcaatctc ctgaccgcta
    12841 agacctgttc ctaaagtttc atgcgagtat tgttcaaact ttcgctttaa ctgggcactt
    12901 accaaccacc caacaagcat aaaaacaatt actataagat atattcccat ttattgattt
    12961 ttttaaattt tagactgctt ttttactaat tatttgtaac tacaatcaaa cagtgtgcaa
    13021 atatacacaa cctgcatttt tgtcagtatt ttttatgatt attattgaat gaattactcc
    13081 caacgaaatg ttttggcagc tataatgtaa ataataacac cccaaagcag caataccaat
    13141 aactgtgata cgacatctat taaacctgca ccttcaaatg caactttgcg caaagcatcg
    13201 ttaagatagg ttaaaggtaa tgctttacaa aacggttgca gccattttgg aaaaacatca
    13261 atcggaaaaa atgttcccga caacaaaaac tgtggcatgg taatgatgtt ggcaatggga
    13321 ggaatagtgc tttcactttt tgctattcct gaaaccacaa agccaaagcc cataaaaacg
    13381 agaagtccaa aagcacagat aagcagcatg gtaagaaaag tacttatgcc gttaatgagt
    13441 gtaaagccaa atgcaaatct ccctacgcct atgatgaaac aagcaccaag taatgaaaat
    13501 accattcggc ttaatgtttc gcccagaaca atataaatac gttttacagg tgtggcaaaa
    13561 aatcgtttta tgaccaaggt ttgtctgagg ctgaaaaaaa caaatgcagt tccaaaaaca
    13621 cctgttgaca atagcgcaaa acctaactgg ccaggaagta taaaatctat tttcttgtat
    13681 tcacgtcccg caatttcttc gctttttaac tgtgctaatg gatgtgccac atgtgcatcg
    13741 tataaattta ttttgcttat aagattatct aaaatatttt tagatagtgc tccgccttca
    13801 ggtgcagcgg tggaacttat aagttttgcc gtataatcag ttttgccttc agtaacattt
    13861 tttgtgataa ccaacatacc atcaatcttt ccttgctgaa gttttttact tgctactgca
    13921 gaatcgcctt caacccaatt aataacatca atattttttg tggcttcata aatcggattt
    13981 tgatgatcgc ttgaaggggt tagaaataat ttgactttaa gccctgtacc cgataaaaat
    14041 ccaaaaacca aaataaaggt tagcggaaaa accagggtaa aaactactgc actggggctg
    14101 cgtgtgattg acctgaaact ggcttttgca atagccagca tggcctttgt ttgagaataa
    14161 ctcatcttat ttgtatcaga aatgctgcaa atgtagttaa gttggttttt atgaattaaa
    14221 ataaaaaatg tacgtttgcc gtaataacat aggttcgtca attcaatgaa tcacacattc
    14281 tcattgtttc agagcatgga aagggataag attatccttt ctttcaaagg agatattact
    14341 caggatttgc ttgcatctgt atatcagatt atggagtcga gaatggatca ggacaagact
    14401 gattccaaga gaaagaaaag attttatcac attttagtcg agtgtcttca gaatgtatat
    14461 caccacatgg aagatttcag aaatgtgaca gaattagatg aaatttctga tggctctgca
    14521 atttttatca ttgccaaagc aaaaggtgag ggctacagaa ttattaccgg aaactatatc
    14581 aagaaagaag aatcggaaga ccttaaatcg aagcttgaga aaattaattc tatgaacacc
    14641 gaagaactaa gagcctatta tcttgaaacg ctgagcacca cagagctttc gcagaaaggc
    14701 ggtgccggat taggaatgat tgacattgcc cgaaagtcgg gacataaaat agtttatagt
    14761 tttgacggag taaccgacac tgttaatttt tttacccttg aagtaacaat taattaaatc
    14821 aaagaaaatg gataagctga ttattgaacc gggcataaaa acacctcaca ttatttttga
    14881 cgctgccaat ggaagtcttg aaattaaagg taaatccatt ccggaaaatt caattgaatt
    14941 ttataagccg gtttacgatt ggctcgatac ttatgcaacg cagcccaaag atcaaacctt
    15001 gctgacagtg caattagaat attttaacac cagctcttct aaatgtttgc tcgacttgtt
    15061 caggaaactt gaaaacattc attcttcagg taaaggaaaa gtaaatgtaa aatggcttta
    15121 tgaggaagat gatgaagaca ttatggaagc cggtgatgac tatcagacca tcgtaaaact
    15181 tccttttagt atcgagaaaa tttcaggata attactttcc ttcaaaaatt tcgtcaatag
    15241 tttttacagc aacagcatga taacccggac gtgccgtttg gtaaactttt tctatccatg
    15301 gttttaatgt tgtatctgct gccgcttttc ggtaaagtgg ggcaataaat tttctgcgcc
    15361 ctactgtgtt tagaaaattt tctaatgctt gatatgcagg cttgtaatga caggcaatag
    15421 ctatcataaa ccaatcaaat gcaatttccg aattttgaat ttgcgtgaaa tgaaaatgat
    15481 tatccaaagc aacagctttt tcgtaggtca tctcaggagc aacagtacgc agaaaatgca
    15541 ggtaatgatg tgttgtccat cttgtagtat caggtattgc agaagttttt ataaatgcag
    15601 ctgcagcttt ttctaccgta tcaaaataac ggcttttaat tacaggaaga cttttaggta
    15661 atcctgttcc ataaacccat tcatcaacat ttactttttg ctttatgagt ggattctcct
    15721 gcagcagatt ggcatctaaa tattttacaa atttctctgt tgtcatagtt tgaaatcgat
    15781 gctcttcaaa atactttttt agaaatgcat cccatacatc acggccagct gcttcttcaa
    15841 gtgccatcag aaaaaacctt cccttttcat aagcaatatc acccatgcca tcatcaggat
    15901 cgcggccttt gaggtcgaga aaaagtttag tatccggatt gttatcaccc ataacggcaa
    15961 cggtatgttt cagttcattt tgcgaaagca agttcagcat gtcggcataa tcttttccat
    16021 aaattttttc catgatgcgc gattcaaagt aaaccgtaaa accttcattt agccaaaagt
    16081 cgttccatgt agcgttggtc actaaattac cgctccatga atgtgccaat tcatgcgcaa
    16141 taaggctaac caatgaacgg tcacctgtga tgattgttgg tgtcgcaaat gtgacacatg
    16201 gattttccat cccaccaaaa ggaaaggaag gtggtaatac taaaacatca tattgtatcc
    16261 agggatactt tccataaagc tgctcagcag atgcaatcat ggcgggtaaa tctgaaaact
    16321 catgaaaagc cttgcccaac ataacaggtt ctgcgtaaat gccacaacgg ttgtcatagg
    16381 ctttgtaagc aaggtcgcca atgcttaagg ccatcaggta tgatggaaca ggcaagggca
    16441 ttttgaatga atagttgcca ttgccaattt gtttggggtt ttgcggacaa ctcatcaatg
    16501 ccataagctc tttaggacaa tgaatggtag ctgtccatgt gaattttatt ccaggtgtat
    16561 cttgcaacgg tatccagctg cgtgccagaa ttgcctctga ttgtgtgaat aaaaacggat
    16621 atattttacc tgctgtttgc tcaggtgata gccattgcaa tgcacgtgca tcgggtgatg
    16681 tttcataatt aatagtaacc acttttgtgc ttgctgtaac aggaatggca agctcactgc
    16741 ctaaaatttc atctgcaggt tttaaaataa atttaacagg tgcatcattt acttttacac
    16801 tttctatttt cagattgtag gtgtctaaac gtaatgtgtc ggcatgggta agattcttta
    16861 cttgcaagtc aacaaatcca actaattttt tagttgagaa atcagcttta agattcagat
    16921 caagatgctg cacaacaatt tctgcagggt tggagtaaga gtgtgcatct ttaatattta
    16981 ctcccctttc atctcccggt ttatcagcaa acatggcaaa tggtattagc attaaaaata
    17041 aaatactttt cattgattaa ttattatgtt ctaaacatat aaatgtgtgg aatattatct
    17101 tccataaatt cttcactaat gactgtaaat ccaaatgatt catagaattt acaaagataa
    17161 ctttgtgcca taatttttat tgggctgttt gggaataagc tcctacattc atctaatgct
    17221 ttttcaacca caagttttcc caaacctgtt cgtcttagtt gttgatgtgt aacaatgcgt
    17281 ccaattgaaa cttcatcata caaaacaccg ggaggaataa ttcgtgcata gccaacagtt
    17341 tgttgatttg ccaaagccat cagatgaaaa gaaagtttgt cggttttgtc aatatcacta
    17401 taaggacaat tttgctccac gataaagacc tgctgcctga tttgtaaaaa atcatgcagt
    17461 tcatgtgctg taagatcatt aaacggttta aacttccagg aaattttcat tctatgatgg
    17521 catgataagg cagcgaatat aaaacacatt ttgtgaatag aaaaagaagt aatatgatgg
    17581 catgctttgt tagcagcatt ttgatgatta acattatttg catcaaacta attaaataac
    17641 tttggcgcca tgtcatggcc acaacttatt gttactgaca gcagccgaag tggaaagctt
    17701 ccttttgctc ctttcaaaag gacacaggaa gagtctgcac agcagccaaa ggccgataaa
    17761 ataatcaaga aggttgtgag gcaaaatctc cctataaaaa ccgatagtat aaatgttact
    17821 gccacggttg tagatacaat aaaacctttt gcaaacgata gtgttcaagc tgccaatgat
    17881 tcttcagttg cagatatgtc gagattgttt cgttttactg atgaggaaaa tgatgaactg
    17941 aacaattcag atcaacaatt tatttcatct tcggcatatt tcaggcagca tcagcttgag
    18001 gtaaaatctt tatctccaca attatttaat aagactgttc cggattggtt taccatagtc
    18061 attttttttc tgattgtggg cattacaaca gtaaaagttt tttatcagaa tattttcagg
    18121 cagttggttg aggctgttta cagttttgcc gttaccaatc aggtggtgcg cgatgaaaat
    18181 ttgttagtgc aacgtgcatc agttttgctg aatgtaattt tttattgtag ctccggtttg
    18241 tttttttatt ggattagcgt gcgctacaat tggaatcatc caatcttaaa tgaagggttg
    18301 ttgcgttttt tgttttttgc tttcgtgaca gcagtttttt ttactgtaaa aatgttgttg
    18361 ttgaaacttt taggaacaat ttttaatctc gataaaccgg tagctgtttt tattttctcc
    18421 atttttttga ctaacaattt attgggtgtt ttactggtgc tgattacttc atttatggca
    18481 tatttatccg gtgtgagtct gataaattac gtctatctgg tattaggaat tctttctctg
    18541 gcatttgttt ttttattgta cagggcattt ttgatttcac ggactgtgca gggttattct
    18601 ttgtattatt taattttata cttttgcacc cttgaaattg taccgattgt attgataata
    18661 aaacttgcat cggagtaatt aaacaaggtg attaaaaaag catggccgga aattcgaaag
    18721 taaagacaat actcgtaacg caacccaagc ccgaaacgga aaagaatcca tactttgatc
    18781 ttgcaaaaaa gttcaatgta aaaatagatt tccgtccttt tattcagatt gaaggaatac
    18841 ctgcaaaaga ttttcgcaaa gaccgcgtta acgttgcaga atttcaggca gtgattttaa
    18901 caagtcgtca ggctgccgac cattatttca gaatggcatc agaaatgcgc atgactaatt
    18961 ttgatgaagt gaaatttttc tgcgtgtctg agtcaactgc cttttatctc cagaaatata
    19021 ttacttaccg caaacgcaaa gttttttatg gcaataacag cataaacgat ttgactgatg
    19081 tgttgaagaa acacaagaaa gaaaagtttt tgctgccttg cagcgaagta atgaatcccg
    19141 acatcattaa taccttaaaa gatggcgacc ttaattttat aactgctgct atgtacagaa
    19201 ctgttagcag tgatctttca gatgtgaata ttgagtcgtt tgatatgatt gtgttcttca
    19261 gcccatcggg agttcagtcg ctgttgaaga attttcctaa atacaaacaa ggcaatacca
    19321 gaattgcaac atttggctta agcacatcgc agactgcaaa agatgctaag ttaaaagtag
    19381 aagttgaagc acctgttccg caggcaccaa gtatgacaat ggctattgag cagtatctga
    19441 aaaaagctaa caaataattt tattatttaa tctcactgag tttttgctga gccgctgtgg
    19501 cataaaatcc tttagccgaa attattttct gcaataactc ttttgcttta tccatctcac
    19561 ctttttgcaa cagcagcaac gacctgtgcc atgcagactc ctgattaaat gtattatttg
    19621 attgcgcatc taaacggtcg aggaaatgaa gtgctttatc atactgctga ctttcgctat
    19681 aagacattgc cgtataaaac aatgcattta catcgctatg attgtctgaa agtaagagtt
    19741 gcaatttgtt aatcgcagaa gcgtatttct tattgttgta atcagccaat gcttcagaca
    19801 aaaagtcctg atatgctatt gtgcgagact gcatgttgtt taacgttttg gatttttcca
    19861 tatcctttct cgattcataa cgggcaggaa tgccattgtc ttcctttgtt atgttctttt
    19921 cgcgtttgta ctcaccggaa tagtcataca cttttagtcc ctcaatattt tttgttgccg
    19981 atttcacagg agcaacagca cctgccgatg cagactcagt agcgatgggt gctgcaacat
    20041 ctactgacgg tggctcggca actgcctcat tgttctgaac aggttctgtt gcagcaaagg
    20101 ctttatcctc tgtacttgct tttgctacca ttgcattttg tgctgccata atattttttg
    20161 atgcttgttg cattttttct tctgccggag gaggaggtgg ttgaggttga atggtagatt
    20221 cattcaattt agcatcgctg ttggatggcg gtgtttctgt atttagtttt tgttgtgcag
    20281 caggtgtact aatatgttcc gctgtgtttt cttgtttgaa agaataatta gaaataatcc
    20341 atcccgtagc aattaaaatt gcaacagatg ctgcagcata ataccatctg tttattgatg
    20401 gtgtttgctt tgcagaagga gaatatttat ccgacacctg tctgatggtt tcatcaagcg
    20461 gtttcatcgt ggtcaacaag gcaaggcctt ctgctgcatc ggaacacagc tcacagtctg
    20521 tcaggtgctt ttcaatggca tgcttttcgg catccgtcat ttttccctgc acatactttg
    20581 tcagtctctc tttaccaatg cagtcagagg ttttaaatat ttcaatttta cgacttgaca
    20641 tgattatttt tctccatata aattctgaga tttcttttcc cattctgaat aaaactcttc
    20701 acctgcttga aatcaaaacc tgtaaggtct gttatttcat tatagcattt ttctttcaga
    20761 aaaaacaact caatacatat tctttgttct tcattcaatg tttttattgc ttcttctagc
    20821 atcaccaatt ctttttcttt ttcatagact tggtcagtat tttctgttac aaagtctgtt
    20881 gttgcagcaa tttcatcgcg gttgtaaatg tctgctctgt tttttgctgc ctgtttgctg
    20941 cgcaattgca tcagacaaaa gttttgtgcc acacgatgca cccagtacga gaacttctgt
    21001 acatcatgtt ttaacaaatc ggtataaagt ttttcaaaaa tctgcaatac ggcatcttta
    21061 ctgtcgtcaa catctttaag atacttcata cacacaccaa atactaaatg agaatagcgg
    21121 ttaaacagta cacccacagc ttgtttatca ccttgctgac gatattgcaa cacaagctct
    21181 tcatcgctaa gcgcattctt atccttactc cggatgagcc acataaataa tttttacttt
    21241 ctgattaatg gtaggatgcc ctgctaaaag cgattccata aaataaacac aaaatatctt
    21301 aaaaaattaa agcactactt cttgtatgac atgaatagga atatcttttc ctgcttttaa
    21361 ttccacatga tcgttggtga cagcccaaac cgttgtttct accgcacagg tgccttcagt
    21421 ggtttcaaac acgatacgca ctttttgttt gtaatcgttt cctaatgcca tggcgccatc
    21481 gagcaggatt tttcgccttg cgcgatcaat ttcattcgat aatacttcgt gttgaacaaa
    21541 atggtagtca gataaatctt ctttagcaat gatggtgagt tccatattga gtgatttttg
    21601 atacatcaga gttttagcgg catataaaag taggcattaa ttccgatata ttcaataaaa
    21661 ttttaaaacg gccatttttc ctgacttccc ggccatccag atctgttttt gtgaaggcgt
    21721 tatactgata gtgtaaaatg ttagcgactg tccatcagcg tttttaatat ccttaatgtg
    21781 gtgttgactg tcaaccatat acgtgccatt atgcccggtt acaatcaggt agttgtcatt
    21841 ttttgttttt attgttttga tgcatgacac aaaagcacca tcaggaagtt ttatgggttg
    21901 ccatgtcttg ccaccatcgt ttgtttggta taaggttgtt gtagtgtctg attgcagata
    21961 attgcctccg ccaataaagc cattgagttt gttttcaaaa tgcatggtaa aaattcctgt
    22021 catagtgctg ccgctaatca taggtgtgtt tttcggaagg aatgcaccgt tattttcaga
    22081 aataaaaagt cttgatgtgc ttcctccggt agcaaaaatc acgctgtttt gcagccattg
    22141 tatgttggtg ttgcttgcag caaagcaacc ttcacctttc attgcaggag atgtaacttt
    22201 tagctcctgc cagttgttgc cgccatcggt tgttttaata atcataaaat gtccatctat
    22261 ggggtcgctt atggcaatgc cttgcaaaga atctttaaat gtcatgctgt caaaaaaaac
    22321 acccttgctg tgattgctgt aaacactttg ccatgatgca ccataattat ttgttctcaa
    22381 tagatatgcc ggtgaatcaa tgctcaataa caatactgtt gaatcgttta aagcagctat
    22441 agatctgaac tcaggatact ttccatcaac ggcagcagag tctatatgcc agttgtttcc
    22501 accatctttt gtaaaaccaa aaacaccctt gttagccgca aaccacacca cactatcgtt
    22561 tactgcctgc agggcacgaa tacttacctg aaccggcaat tgtatcgttc ttaattctat
    22621 ctgttgtgcc ttcagtgata atgtaaaaca aaagcaccac agtatgagaa aacttttttg
    22681 cattgtgttt caaatgtagt gttttttcag gcctgcaatg gttgtataag taatacaatg
    22741 taatttttct taggactatt ctctttccct cattcccgcg aagctcctgt tttgtggggc
    22801 aaggtgctta atttattaca taaaaagtta aatgcttatt ttaaaaataa ggaagtaata
    22861 aaagtgctta tattgtagcc atcagaatgt ttgtattgta atcatgtcag aacagcttaa
    22921 ccgtgtcata gcacacatgg atttggattg tttttttgta tccgtatcgc ggctgttgca
    22981 tccggaacta aacaataagc cggtaattgt tggcggtggc gagcgtggtg tagtggctgc
    23041 atgcagctac gaaaccagaa aatttggtgt gcactctgcc atgcccatta aacaggcaaa
    23101 acgactttgc ccggaagcta ttattgttcg tggcgattat gatgcttaca gcaatcattc
    23161 cgatatggtg aatgagatta tttgcgaaag tgtgccgctg tatgagcgca gcagtattga
    23221 cgaattttac ttagacctaa cgggcatgga tcgttttttt ggatgctata aacatgccac
    23281 agaactcagg caacgcatca tgcgcgaaac agggctgcct atttcttttg gtatgagtgc
    23341 caacaaaacc gtaagtaaaa ttgctaccga tgaagcaaag cccaacaatc agatgcaggt
    23401 gaactgtggc gaagagaaat tttttttagc tccgctgtcg gtacgaaaaa ttcctatggt
    23461 gggccaaaag acctacacac tgctgcgcag catgggtgta gagaaagtaa aaaccgtaca
    23521 ggaaatgcct gttgaactga tggagcgtgt gctgggcgaa aacggaacta ccatctggaa
    23581 aaaggccaac ggcattgaca ccacccccgt agagccttat cacgagcaaa aaagcatgag
    23641 cacagaagaa acttttgagc aggacactat tgacgtgcaa cgtctgcaac atattctcat
    23701 tgccatgaca gaaaaactat gcttccgcct gcgcagcgaa cataaggtca cctcctgcgt
    23761 tgccgtgaaa atacgctata gcaatttcga cacacatacc atgcaatgcc gtattcccta
    23821 caccggctgc gaccacatca tcatcaaaca tgttaaagat ttgttcggca aactgtataa
    23881 ccgcagactg ctcattcgtc tggtgggcgt aaagttcagc cacctggtgg ggggcggaca
    23941 tcaaatcaat ctgtttgaag acagcgaaga aatcatcaac ctctatcagg caatggacaa
    24001 cctgcgcctg cgctttggcg aagacaaaat tcaacgtgcc gctgctatga atttttcact
    24061 gcgcggcttc aatccgttta atgggttgag taaaaaaccg gaaagcaaat aaaatccgga
    24121 agcgtgttaa atattaagaa gggcgaattg atgttaaaaa gtagaattaa tttatcatca
    24181 ataccaaatc aggcaacaag ccgagctttc catttttgag tatattgcaa ctttttatat
    24241 cactgttgga aggtacaaac agttaaagaa cttaactatt ctaaagcatc aacaattatt
    24301 aaacaacact aaacatgcta caaaaggtta taaattattg tccacttttt tcttggaatt
    24361 ccaaataacg aatctgttac gttccgaacc atcggaatta cgaatagaaa aataaatact
    24421 gtttaaagaa acgctttgcg aaatcggggc gtttttttgt tgggagaaat gccaaggaaa
    24481 aatatttatc aactgcttga atttttaata atattttcat atacatttac actgtttttc
    24541 tttttcaggt gtaattaaaa atttggttac atgattagaa ggtggttgct ttcattggta
    24601 ttttttttat tgcttttacg cggtaacgca caggtggcga atgatagttg tttatctgcg
    24661 acaaatttag gtttacttcc aataggttct gttttaagtg ttccttgttt aagtggggca
    24721 cctgaaattt ttattgcaga ttcaaccaca atggctgttg ctaattatcc ttatccgagc
    24781 attcctaacg gatgtaacgg atacgtgagt acagttgcct cacctgcaaa agacagatgg
    24841 tataaattta cggtaaactg cgattttact tttagggtgg aaaattcgga cactctgcac
    24901 ttaagttttt ggttgggtga ttcttgcagt aatttactac caattgaatg ttttactata
    24961 cctgctaata caacttttat tcaagcacta tctgggttag gaggacattg gatttatttg
    25021 caaataagtg gacaagataa cataaatgca aaccattata atatttgttt ttctacatca
    25081 attccaatgt gtgcaccttc ttttaatttg ggtgctttta ctcctgtgct atgttttgat
    25141 tataacctgc aagttataaa ctctacaagt gcttcgataa atgatggagg cattattatt
    25201 aatataactg aagggaaccc tccttacact tgctcatgga gtgatggtgc aactggtttt
    25261 ataagagata gcttgcagat agggcattac acttttacaa ttgttgatgg gattgggtgt
    25321 gaattgacag actcggtgac agtgggcatt ataactatcc aaaaagatat tattgaaaat
    25381 gatttacatg aaataaaaat tttgttcaac aatttaaacc ataccctatt attaaaaagt
    25441 aatagctcgt caattattgg tagagaaatc aacttaagta tttttgatat aggcgggcaa
    25501 aaggtattta ataataaaac aaaagctaat attccttttg aattttattg taaaaatggg
    25561 atctatttat ttcaaatttc cgttcaaaat tgtgttataa acaaaaaaat atatatcaac
    25621 taatcatgtt ctaaccaata gaacccctct ggcgcacccc gatagttatc ggggtgcgcc
    25681 agaggaagac taaaagatgg taaataatct aaaaaatcaa aaagttgtag aataataatg
    25741 ccttgacaaa acaaaattca atatttgctt taacggtctt aagttttaat tataaatatc
    25801 aatagttaaa ctcaggtgcc taagcttaat taagaaagct ttataaaaac tttctgatga
    25861 aaaacttaat accattaacc attaaaagat attttgctgt atttgtttct atattctaaa
    25921 ggtgtaacac cagtatgttt tttaaataaa tccctgaatg acttggtgtc tgtatagcct
    25981 acgtcaaaca tcacttcagt aatatttttt ctgctgcttt caaacttacg ctttgcagtt
    26041 tcaattttta ctcgttgaat atattccagg actgaatgat tggttgactt cagaaatctt
    26101 cgctcaaaag ttcttcggct catctttgta aagtcagcaa gttggtccac tgttattcgt
    26161 acatcagtat gtttttctat ataactctgt gcttttaaaa tttcaatatc cgtatgattt
    26221 ttgtgaccat taaaaattgc aaatgacgcc tgactgtttc gatccaaatc aatagcaaag
    26281 tattttgaag caagtatagc agtatgacga tcagaatatt tttccaacaa ataaaacagt
    26341 aaattccaca gtgagtttgc accaccgctg ctgtaaacac cacatgcttc tgtaatgata
    26401 gcgccatcaa ctacaactgt atttgaaaat aaatcattaa actcattaaa ataagcccaa
    26461 tgtgttgaca cttttttacc gtcaaccaat ccggaagccg ctaataaaaa agcaccgata
    26521 cataaacttg caacttctga accctgacga tgcatttttt gtaaccatgg aatggcagca
    26581 gaatttttct ttaaggaatg ttttacgtca ccataaagtg caggtataat tattaagtca
    26641 gaatcattaa cattacttat atgcctatca atacgaatgc tgtattcacc atcattggct
    26701 ttgatgcttt tttgcaagcc tacgtactca actttaaact ttggtctttt acctgaggct
    26761 ataagaatat catttgcagt attgaataat ctgtatgccg gtgttatgac ttctatagct
    26821 gcatgatgcg gtacataaac agaaattaac attccggcaa tttttatttg gtaaagttaa
    26881 aacattttgt cattatcaca ccttaaaact gtcgtttctg ccaccattag ctgttgaaac
    26941 tgttgctcat ctttgtaatg ctaaatcaat cattcacttt taaacaaaaa attatgcaaa
    27001 caaataatgt aaatctacac agggtttttg cttctccaat agagaaagtg ttcagagcat
    27061 tttctgatgc cgatgcctat tccacatggt tgccaccgta tgggtttatt gccaaaaccc
    27121 atcagtacga ttttaaaaaa ggaggcagtt ataaaatgtc gtttgttaat ttcaccacag
    27181 gttttattca ctcattcggt ggagagtttt tagaaattaa taccaatgaa ctcataaaaa
    27241 ttacagaccg ctttgatgac ccaaacctac ccggtgaaat gattactact attcaattta
    27301 agaaagtgag ttgtggtact gaagtgaata tttcgcaaac aggaattccc gaagctatac
    27361 cttcagagat gtgttattta ggatggcagg attcattgga caagctaaaa agattggtcg
    27421 aacctaatat acctgatgcc tgatttttct gtattaaatt taaacaaaca taaatcaata
    27481 taataacaaa attcaaaaaa caatcattat gctaaaacaa gtatttataa atcttcctgt
    27541 aaaggatctg gaaaaatcca tgtcatttta cgaagcaatt ggtttcacaa acaatcctca
    27601 gtttacagat catactgctg cctgcatggt ttggagtgat actatttttg taatgctttt
    27661 aacgcatgct aaatttaaac agtttacatc caaacaaatt attgatgcaa aacaatcagt
    27721 aggtgtttca aatgcactgt catttgaaag tattgagact atgaaaacat ttgcagctaa
    27781 tgcagtcaag gccggaggca gagaatatgc ctcagcaagt gattatggtt ttatgcaaca
    27841 atattttatt gaagatttgg atgggcacaa ttgggagcca ttctatatgg atatgagtaa
    27901 attcccagga taaataacaa actataaaat taagaaatat ggcaaagaaa aaaatagctc
    27961 catgtttatg gattgaaaag gatgctaaaa aagcggctaa atattacctc tcaatattta
    28021 aggatggcaa gatgctttcc tataaggcat ataaaaactc aggtacgccc agtggcgatt
    28081 ttgatattgc ttatatcaat attctgggaa tggatgttca gattcttgct gcaggacctc
    28141 tgtttaaatt tagcgaagca gtatcttttg ttatcatctg caaagaccaa aaagaaattg
    28201 attattactg gaaggcatta acaaagaatg gtggcagcga aggcccatgc ggatggtgca
    28261 aagacaagta cggcctgtca tggcaagtta cccccgagca atatttcaaa ttagaaaatc
    28321 ataaaaacaa agccaagcag gaatatgctt tcaaagcttt gatgaaaatg aaaaaaatta
    28381 tcattgccga cttagtagaa aacaagtaaa tttcagcgga actataagat tcaataacgg
    28441 atgaataaaa ttgctgtgag tacctctatc agtgaatcaa taagaaaagt gtgggattac
    28501 tatacccatt ccaaccctat tgttaaccgg aattttgttg aggcatgatg gcaggctatt
    28561 ctgagcaatt ttaaaagttg taccgagcat aattaatcca tgcaattatg tgcgtatatc
    28621 cattttaatg gtaattgcaa aaaagcaatg accttttata aaaagtgtct tggtggaaaa
    28681 ctgagtttcc aaacggttag cgaattgccg cagtcagagt cacttccttc ctatatgcaa
    28741 aggagtattc tgacagcttt attacaaact aaacattttc agttagcagg aacagacctg
    28801 aacaatgaat cagtttattt gcctgacaat agtttttcgc tgctcttaca atgtgatagc
    28861 gaatcggata taaagcggtt gtacaggcaa ctttctgcag gtggagtgaa gacacagttg
    28921 cttcagaaaa atttttatgg tgtagtaatt ggttctgtta aagataagtt tggcattaac
    28981 tgggtgctac agtgctgatt gcactgtttt ttgaatttat tttctttcag aaaatttgtt
    29041 gcagtttttc ttaatgttta tattacaacc gaccgaatga ttgcaatgta aacatattgt
    29101 atgtgtgtag cggcagttat cgggttgctt caatatgttg ccatgcacct ctgttgtcac
    29161 tcatacttct ccttaaaata cggcaccatt agtccggaag atttgttatg ccttgccgca
    29221 caaaaaaata ttcggtcgct ggcactcact gatatcaaca acacctcggg catactcgat
    29281 ttttttcgtt tagcacaacg atataatgtt tgtcctgtgg caggcattga tttcagaaat
    29341 ggtgtggagc aaaaatttat cggcattgca aaaaacagcg aagggtttca tgagctcaat
    29401 aagcttttat cagaatattt attttccgga gaaagattct cagacaaaac tccggcactc
    29461 caacaggcat ggatagttta tccattgttg tcggcaccac aacagttgct cgaaaacgaa
    29521 tttgtaggca ttaaaccaac tgaacttttt cagttaaact tttcgcagtg gaaaagtaag
    29581 ttacacaaac tggttgctct tgcaccggta acttttcgca acaagacaga ttttaattca
    29641 caccggctgc tgcgtgccat cagctacaat acgctgctca gcaagctgcc cgttgccgga
    29701 caggcaccgg cagatgaaat tatgtattca ccggaagaga tgaaatacat ttacaaagaa
    29761 tatccacaac tgatttacaa cacagaaaaa ttattggagt cctgtcatat agattttgaa
    29821 ttcggaaaaa acaaaaacaa aaaatatttt ttgggcagtg ccaaacgcga tcatcacaaa
    29881 ctgatgagtc tttgttatga gggtttaagt tatcgttacc cgaatactga cgacaccatc
    29941 attgaccgtt ttgaaaaaga gattcagatg ataggtgagt tgggtttctc atcatatttt
    30001 ctcatcaacc acgacattgt gcgctatgca cgaagtaaga attattttta tgtaggccgt
    30061 ggcagtggag ccaacagcat ggtagcctac ctgctgcgca ttaccgatgt tgaccccata
    30121 gacctcgact tgtacttcga gcggtttatc aatccgcagc gcaccagccc tcccgatttt
    30181 gacattgatt tcagctggaa cgaacgcgat gatgtgattg attacatttt taaccgaagt
    30241 acttttggca gagaacatgt tgcgctgctg gccacctaca gcacctttca gcgcgatgca
    30301 gccatccgtg agttgggaaa agtttttggc ttacccaagg cagagattga ttcgtttttt
    30361 gaaagtggaa atgccaaacg cgaccacatc agtaacttag tgttgcaata tgcgcagcgc
    30421 atcatagacc tgccttcgca cctgagtatt catgcggggg gcgtacttat ttcagaaaaa
    30481 cccatgacgt attataccgc tttagataat ccgcccaagg ggtttccact tacacagttc
    30541 agcatgctcg aagcagaaga tttaggtctt tataaatttg atatcctgtc gcagcgtgga
    30601 ctgggaaaga ttaaagatgc tgttgctatt attgaaaaaa accgacaggt aaaagtagat
    30661 attcacgaca tcaaacggtt taaagaagat gaggctgtga ggcacaacct tgagcaggca
    30721 aacctgatgg gatgttttta tgtggagagt cctgccatgc gtatgttgct gaagaagcta
    30781 aaagccaaaa cctatctcga cttggtggct gcaagttcaa tcatccgtcc cggggtggca
    30841 caaagtggca tgatgcgcga gtatatttta cgctttcatg atgagagccg cagaacctac
    30901 atacatccga tgatggagga gctgatgcgc gaaacttttg gggtgatggt ttatcaggaa
    30961 gatgtgatta aggtagcaca ttattttgcc ggacttaccc ttactgaagc cgacatgctg
    31021 cgcagaggga tgagtggcaa ataccgtggc cgtgcagagt ttgagaaagt aaaagatgct
    31081 ttttttaaag gctgcgcaca aaagggacat gcgcccgaca ctactgctga ggtgtggcgg
    31141 cagattgaat cgtttgccgg ctatgccttc agcaaggggc actcggcatc gtatgccgta
    31201 gagagctatc agtgtatgtt tttaaagacg tattttccgc ttgagtatat ggtggcatgc
    31261 atcaacaacg gtggaggctt ttacgcagct gaattttatg tgcatgaggc ccgtctgaac
    31321 ggaggcatta ttctggcacc cgatgtaaac cacagtgctt atgaaaccac catcagcgga
    31381 aaaaatgttt atctcggatt tcatttggtg ggtgagctgg aaacaaagac ggctgaaaat
    31441 attttgaatg caagaacaca ggaaggccct tttacttcgc tggcaggttt tatgcaacgg
    31501 gtagaaattt cattggagca gttgcggctg ctcatacgca taaatgcttt acgttttacg
    31561 ggcagaacta agcagcaatt gctttgggac attcacgaaa tgataggtca tgaaaaaaaa
    31621 acctgtacgg caaggcaatt atttgaagcc gaacaccaac attttgcact gccagagttg
    31681 catagcagcc aaacagacga cacctgggat gaaatagaaa tgctgggctt tccgttaggc
    31741 tcgccatttg atttgctcat ggcagagccg ctcccatgca atgaaaaaaa tattattaca
    31801 ctggcttcgg aattgccgca gcattgtggc agaaccgttg ctattgccgg ttattttgtg
    31861 acacgcaaac ccacacgcac caagcatgga cagcccatgg cttttggtac ttttttagac
    31921 cacaacggac agtggattga caccacacac tttcctaacg tagtgaagca atatcccttt
    31981 cgcggcaaag gttgctaccg catcaccgga aaggtggtgc aggagtttgg tttttacagc
    32041 atagatgtca cctctctgag tttgttgccc tacaaaataa ggtttgccga tgctgccgga
    32101 aattgaaacc aaacccactc tgttatatac aggtgaagaa aacgtgcttg aaattccgct
    32161 actaaaatca atccctgcgc tatcaccaaa acaccacata ttgttttggt tttgagctat
    32221 ggcatttgca cttagcaacc ctttcgggct tggagaaaga tatatacctg gcagaacgga
    32281 ttgtttataa ccggcagtct tagaaaatgt atagctatct gaaaaacaag gacctacata
    32341 cgaccttagg tttgttaaaa acctttatat ttgccgctct taaaaaaata ccgaaaaaat
    32401 caattattca tgcaaagcaa aggcgcaatt aaatttttag ccattctgat tacaatcatc
    32461 tgtctgtttc agttgtcttt tacagtagtt acaaaccgta tagaaagcaa ggcacatcag
    32521 gcaactaatg gcaaccccga agcatacaag gcatacattg actctatcag tcagttaaca
    32581 gcttataatc tgggctttaa aaaatacacc tatcaggaat gtaaagagcg tgaaatcaac
    32641 cttggtcttg accttaaagg aggtatgaac gtaacgatgg aagttagtgt tactgacctg
    32701 attcgcaata tggccaacaa tcctaatgac ccaatgttgg tgaaagccct ggatgatgcc
    32761 catcaggcac aacgtacaag ccagaaagga ttggtacaat tgtttggcga agcctttgaa
    32821 cgcaacaatc cgggagcaaa attagcaggg ctttacagca ccatggattt gcaaagcaga
    32881 gtaaatttta actctaccaa tgccgaagta ataaaggtta ttcaatcaga agcagatgct
    32941 gctatcagcc gtacctacaa cattcttcgc acacgtattg ataaatttgg tgtaacgcaa
    33001 cccaacattc aacagttagg cggaggacgt attttgattg agctacccgg agtaaaagag
    33061 ccggatcgtg tgcgtaaact gttacaagga actgcacaac tggaattctg ggaaacctat
    33121 gataatgcag aagtgtttcc acagttagaa gcagtaaata aaaaacttgc tgcacttgaa
    33181 actccagtac caacagactc tagtgcaaaa tcagatacaa ctgttgctgc tgccggtgat
    33241 tctttaactg ccgctgcaga tacaggtgca atgaaaaaac ctgcactact cagcaaatta
    33301 gagagtgata ctgctgcttc taaagacact tcttccagtg cacagttcgc aaaattctct
    33361 aaagagaacc ctctttttgc actgcttaca cctgcaattt ttaccaatgc acaaaaccaa
    33421 agcgaatacc gcaaaggacc ggtagtgggt tatgctgcta ttaaagatac aggtaagctg
    33481 cgtgaaattc tggaacgccc cgaaataaaa aatattcttc cacgcgattt gcgtttgttg
    33541 tggacagcca agccaccaac aaaagaatca aaattcttag agttagtagc cattaaagta
    33601 aaaagccgcg atggcaaagc accaatggat ggctctgtaa ttacagatgc ccgacaggat
    33661 tttggtcagt ttaacagctc acctgaaatt tctatgtcaa tgaatcctga aggagctaaa
    33721 acatggaaaa gacttacagg cgaaaacgtt ggaaaaagta ttgctatcgt tctcgataat
    33781 tatgtttatt ctttcccgaa tgtacaggga gaaattgcag gtggtcgttc ttccatcacc
    33841 ggaaactttg aaatttcaga agcacaggat ttggctaaca ttctaaaggc tggtaaactt
    33901 cctgctcctg cacgaattgt tgaagaagca attgttggtc cttcattagg tgaggaagct
    33961 attcaaaaca gttggatgtc gtttgctggt gcatttgttg taatcctgtt gtttatggga
    34021 ttctattata tgcgtgcagg ttgggtagcc aacattgcat tgtttgccaa cattttcttt
    34081 atcatgggag tacttgcatc gttaggtgct gtacttacac ttcctggtat tgcaggtatc
    34141 gtactcacca taggtctttc tgtggatgcc aacattctta tttttgagcg tgtgcgagaa
    34201 gagttaaaag caggtaaggg cataagactt gcagtttctg atggttttaa acatgccatg
    34261 ccttccatcc tcgactccaa catcactttg ctcattctgg gtatcgtgct ttatattttt
    34321 ggttccggtc cggtacaagg ttttgctact acactggtta ttggtgtact cacatcattg
    34381 ttctctgctg ttttactttc acgactcatt tttgaaggta tgctggataa gaacaaacca
    34441 atttctttcg gtaatgagtt ctctaataac atttacaaaa acattcactt caactttgtt
    34501 gataatagaa agaaatatta catcatctct tcaatagtaa ttattgcagg tgttgtttct
    34561 ttcgcaataa aaggtttgaa ttatggtgtt gactttgaag gaggacgcac ctacaccatt
    34621 cagatggata agagtatttc tactgaagac atcagaatgg cattagagcc tcagcttgta
    34681 gaagcacctg aagtaaaatc agtaggtaca acagaccgtt ttaaaattac aactgcctat
    34741 cttattcacg ataccgcaag caatgtagag aatgttattg ttgacaaact ttataaagga
    34801 cttacaccac tttacagtgc agcacctgat tttgaaactt tcaaaacaaa gaatattatt
    34861 tcatcgcaaa aagtaggacc taccattgca gatgacataa agcaaagtgc agtttgggct
    34921 gtattgatct catgtgcgct gatgtttgtt tacatcttta tccgattcaa aaagtggcaa
    34981 tatggacttg gtgcagtagt tgccttgttc catgacgtat taattgtatt gtcattcttt
    35041 accttgctgt caggagttat tcctgtggca cttgaaatag atcagcattt cattgcagct
    35101 attcttactg taatgggcta taccatgaca gaatctgtag ttgtattcga ccgtatccgt
    35161 gaatacctcg gacatcatca cactgcagat gacaaacatg taattaacga agcgttaaat
    35221 gccacgttaa gccgaaccat aaacactaca ttaactgtat tcttcgtgtt gattgtaatc
    35281 tttatttttg gtggtgaggt aattcgtggt ttcatttttg cactcctcat aggacgtatc
    35341 atcggaactt actcttcact ttgtatttct acaccaattg taattgacct tggtggaaat
    35401 gcacatgaag gtgtagcaaa aaaataattt cttaaatagc actttattga aaaaggctgt
    35461 cacaaaaagg cagccttttt tgttgatatt atcctatacg agggttgaag tttaaattta
    35521 tttacacctt acctgtttct gcatattgcc tttttactaa atgtgctgca ataaaatagg
    35581 aagcaagtgc accgatgcaa aaaaccacta aaaagacact tacaaaatca gttacctgca
    35641 tttgaaccgg ataggcttct acaacaaaag aatcggcatt acctaatttg atgagatgaa
    35701 aatgttgttg cagaaaacaa atgatgccac cggcaagtaa gccaccaaca acacccacaa
    35761 gtgtgattaa aagtccctcc atgaaaaata tttttttaac cattgcaaaa ccagcaccta
    35821 aatttttaag aatgataatg tctttctttt tctcaatcac aagcatagtc agcgttccca
    35881 gaatattaaa aataccaatc accataataa atgagagaat gagaaacact gcccactttt
    35941 ctgagctcag gattttatag agaaactcat gttgttgtgc gcggttttta accgtaaact
    36001 catgaccgga aatttctttt attttttgaa taatagcagt ctcatcaaca ccgggtttca
    36061 tcataatctc aattgctgat gcttttcctc cggcagcaat aaattcctga acaaatgaca
    36121 tgggtgccag aatatattta gaatcaaaat cctgctgaat ggcaaaaatc ccaccaattg
    36181 aaatgttcat ttgtcgcaat gcattttcaa aatcaccggg agaggtaatg tttttatcag
    36241 gaaggtaaag catcagtggc tcgttgcctg tgtccatatt tacagacaat gcgtatgcaa
    36301 cgccactacc tacaatggca aagttgcgct ctccgctttt cagaatcggc aagccggtaa
    36361 ttacagcttt gttcaaccct gtttttttca tcagctcgtt gtccatacct ttcaaggtaa
    36421 ctatgtcctg tttttcacca tagcgggcaa ggcagttttc ctctaaggta aaagctgcat
    36481 attgtactcc atttatcgca tttatcttat caagactgat tgattcggta ttaaaagatt
    36541 ttccttcaat tgctgtaatt ttaatgtcgg ggtcgaaggt gtcgtaaaga gaaattacca
    36601 gattgccaaa gccattaaaa acactcaaca ctacaattaa tgccatagta cctgtaaaaa
    36661 cacctgcaac agaaaccagg ctgactaagt tgacaatatt gtgtgacttt ttgccaatca
    36721 gataacgaga tgctatttta ataatcaaat tcatgcttgt gggctaaagt cagcaagaat
    36781 aatcactttt acaacaatga cttaaaaatg gcccttcttt tttgcaacct tagctttctg
    36841 ttaataggca ataatagagc taaatagtat cttcgctgcc tcaaatttgt aatgagatga
    36901 aaaaactcca catggttgac ctgatcagtc aatatcagaa aataaaaccc gaagtagata
    36961 aagctatttc agatactgta acatcatcgg cctatataaa cggaccggag gttaaagctt
    37021 ttcaggcaga gttagaaaaa tatttgaatg ttggtcatgt aataaattgt gcaaacggaa
    37081 ctgatgcact gcagatagcc atgatggctc ttaatctgca acccggtgat gaagtgataa
    37141 cagcaagttt tacttatgtg gctactgctg aagtaatagc attactaaaa ttaaaacctg
    37201 tgttagtaga ggttattccc ggaacatttt ctattgatcc tgttgccatt gaaaaagcca
    37261 tcacaccaaa aacaaaggca atagtgcctg tacatttatt cggacagtgt gccgatatgg
    37321 agcctattat gcaaattgct gcaaaacata aactttatgt aattgaagat gtggcacaag
    37381 ctattggtgc agattacact ttctctgacg gcagaaaaca gaaagccggc accatcggaa
    37441 ccattggttg tacatcattt tttccaagta aaaatttagg ttgtatgggc gatggtgggg
    37501 cgctttttac caatgacgat acccttgcaa agaaaataag aatgattgcc aatcatggtc
    37561 agtctgttca gtatgtacat gacgaaattg gagttaactc aagacttgac agcattcagg
    37621 cagcaatttt aagagtaaaa ttaaaacatc ttgacagtta tgcagcagca cgacaaaaag
    37681 cagcagcata ttatgataag gcatttagtg gtaatgcaaa aataaaaaca ccggaaagaa
    37741 gcaaaaactc tactcacgtt tttcatcaat acactttagt ggttaatggt gttaaccgcg
    37801 atgaattgcg tcaatattta gcagagaagg aaattcccgc tatgatttat tatcctattc
    37861 cattgcacat gcaaaaggca tataaagacg atcgttataa ggaaggaaat tttccggtaa
    37921 cagaaaaact ttgtgccaat gtattgtcat taccgatgca tacagagttg gatgaagagc
    37981 aattggaata tattacccgt gcagtacttg aattttgtaa atagaaaata attttatccg
    38041 tatagtattt taatatgaac attggagttg taggtacagg ttatgtaggt ttagttaccg
    38101 gaacctgttt tgctgaaatg ggcaatcatg tagtttgtgt ggacattgat caggcaaaag
    38161 ttgacagcat gaagcaagga aaaattccta tttacgaacc acatctcgat gtgttgttcg
    38221 agaggaatat cagacaaaat cgtttgtcgt ttactacaca gcttgcagat gcagtaaaac
    38281 attcatccat tatatttctt gccctaccaa ctcctcccgg tgaagatggc tctgcagatt
    38341 tatcctacgt gctgggtgtt gccgaccaat taggaaaaat gcttgatggt tataaaatta
    38401 tcattaacaa gagtacggtg cctgttggta cggcagaagc agtgagagat gctattgcta
    38461 aaaactataa aggcgaattt gatgtcatct ctaatcctga atttttgcgt gaaggttttg
    38521 cagtagatga ttttatgaaa cccgatcgtg ttgtcattgg aacacgttca gagaaagcaa
    38581 aaaaaattat gggagaactg tatgcacctt ttctgcgtca aggcaacccc atttatttta
    38641 tggacgagcg cagtgcagaa ctaactaagt atgcagctaa ctcatttctg gcaacaaaaa
    38701 ttactttcat gaatgaggtt gccaacctat gcgaaaaagt tggtgctaat gttgatgcag
    38761 tgcgcattgg cataggctct gatgacagaa ttggtaagcg gtttttattt gccggtattg
    38821 gttatggtgg aagctgtttt ccaaaagatg tgcaggcatt ggaacataca tccggcaagt
    38881 atggatatga ctttagtatt ttgcaatcgg tgatgagtgt aaacaacaaa caaaaaatgt
    38941 tgcttgcaga aaaagtattg gaatattatg gtggcaatgt aaaaggaaaa cgatttgcat
    39001 tgtggggtct tgcatttaaa cccgatactg atgacattcg cgaagcacca tcactttata
    39061 ttattgacat gctaataaaa gcaggtgcaa ccataagagc attcgaccct gaggcaatga
    39121 gcaatgtaaa aaaacagatt ggagataaaa ttgagtttgc taacgatgag tatcatgcat
    39181 tagaaggtgc tgatgctttg ttgatagcaa cagaatggag tgccttcaga acacctgatt
    39241 ttgataaaat gcaatcaact ttaaaaagca aagtaatttt tgacggacgc aatttgtacg
    39301 aaccacaaaa aatgcaggaa gcaggatatt attacaacag cattggccgc caaacggtaa
    39361 aaccatgaca aaaagaattt taattaccgg agctgccggt tttttaggat cacacctatg
    39421 cgaccgcttt attcaggaag gttatcaggt tgtgggaatg gacaatctca taacaggcga
    39481 tttaaaaaac atttcgcacc tctttaaact ccgtgagttt gatttttatc atcacgatgt
    39541 ttcaaaattt gtgcacgtac ccggcaagat agattacatt ctgcattttg catcaccggc
    39601 atcaccgata gattatctta aaattcctat tcagacgtta aaggttggct ccttaggaac
    39661 acataatctg cttggttttg cattggcaaa aaaagcacgc atattggtag ccagcacatc
    39721 tgaagtttat ggcgacccat tagtgcatcc acaaaacgaa gattattggg gcaatgtaaa
    39781 ccctattggc ccacgtggag tgtatgacga agctaaacgt tttcaggaag ccattaccat
    39841 ggcctatcat acctaccata aattagaaac acgcattgtt agaattttta acacctatgg
    39901 cccacgcatg agactcaatg atggtcgtgc attgcctgca tttatagggc aggctttacg
    39961 tggcgaagac cttacggtgt ttggtgatgg cagtcagtca cgttcatttt gctatgtgga
    40021 cgatttggta gaaggaattt ttcgtttgct tcactgcgac tatgccaaac ccgttaatat
    40081 tggaaatcct gtggagataa ctatcaaaca atttgccgaa gaaattatca agcttacagg
    40141 cacaacacaa aaaattgttt acaagccact gcctcaagac gatcctaagc aacgacaacc
    40201 tgatattaca cgtgctaaag aaattttggg atggacacca aaggtttcgc gtgaagaagg
    40261 tttaaagatt acttacgaat attttaagtc gctaccaccg gaagagttga aacgctctgc
    40321 acataggttt gcatgatgtt ttaatcagga caggaattaa aaacttatcc cacattttgt
    40381 atttagttta gtaaggataa ttttctgttt tatcaccatt tggaaatatt gactttgttt
    40441 aaagaatttt tgtatttgaa aaaagaatta tttcttcttt tctttttgcc tgaaatattt
    40501 tcgaaacaaa aaattctttt gtaatgcttt taaatcttct tcaagaagaa atgagctttt
    40561 ctttaattgg tttaatgttt cattaaaatc atttttcatg atagtgtcag tcagcaaagc
    40621 ctgtacagtt ccttgtccat tgacgagcag accggaaaac tgatttatgt tatttgttgc
    40681 ctcaattaac gaatcggaaa gttgttgcag gttagtaagc aaaactgtta attgattttc
    40741 tgaaatactg tctgcaataa gtttacccaa tactcctttg ccattttgaa catctttggt
    40801 aatattatta atatcttttg taaagttatc aatgttattt gtagccgagt taattgattg
    40861 aatagattgt cttaaatttt caattacagt ggaatcagag ataagtgtaa taagattgtt
    40921 attcttatta aaacgttgta ccaagccctt taggtcagat gtaatcgcag caagattatc
    40981 attagtttta tttaatgtac gtatcatctc atcggattcg acaggacgca gcgataataa
    41041 aacatcacca gcttctattg gtgcagattg ctcaggcagc gagtttatat tgacaagttt
    41101 atttcccatc aacccatctg ttccaatact ggcaatggca tttttcttaa tataacgagc
    41161 catcttcttt tcaattatca tttttactgt aatggctgaa tcactttcaa aaataatttt
    41221 ctgaacagtg ccaatgtcaa tgccaccata acgtacattg tgcccgggca ttaatccatt
    41281 tacattatgg aaaactgcac ttatctcaat tgttttactg aaaacactgc gcttgcttcc
    41341 aatcatatag agggcaatta ccaacactag taatcccgcc attacaaaga atcctaactt
    41401 aatattgttt gcttttgtat tactcatgat gcaaattcaa aaaattgatt aatattttta
    41461 tcagatgatt tttttagttc atcatacgtt ccttcagcat agttcctgcc atcaagcaga
    41521 acgataattt tgttggctgt gagttttgca actgacatat catgcgaaat tattactgat
    41581 gaagtatgat actttttttg caagtcaagc atgagttgca caatttctct acttgttatc
    41641 gggtcgagac cagttgtagg ttcgtcataa agaataagct gtggcttcat gatcaatgtg
    41701 cgagcaatac caattctttt acgcatacct cctgataatt ctgaaggcat catattgatg
    41761 gcatgaagaa gccctacatt atccaacgat tcttcaacaa ggtcttttac ttcagtagaa
    41821 gttaaagaaa tccagtgtct ccgcattggg aattcaagat tttctctaac tgtcattgag
    41881 tcgtacaatg cgctgttctg aaaaagaaat ccaattttag tgcgaatctt atctaacgac
    41941 tcctgattta gctcaagcat attggtgcca aagacatgga tatttccttt atccggtttc
    42001 aataatccaa caatacattt tatcagtact gattttcctg agcctgattt tcctaataca
    42061 acaatatttt ctcctttaac taattcaaga ttaaagttgt taagtactac attactgcca
    42121 aatgctttgc aaagcccttc tattttcaga atgatattag tattgtccat aatcagttca
    42181 agcctaataa atcagtaatc tgaacagcaa ttaaatcaag aataaaaatt attaatgaag
    42241 ctactaccac agctgaattt gcagcatgtc ctacaccttc tgtccctttt tgagaattgt
    42301 atcctttaaa acaacctact agtccaatgg ttaaaccgaa aaagaatgat ttgactaaag
    42361 ccggaataaa atcactgtat tcaagtgaat tgaaaacctt gttataaaac aatttccagc
    42421 ttactacgtc tttaaaatta acaccaatat aacaggcata aatagcaatt aaatcagcta
    42481 gtataaccag tatgggtaac ataaatgcaa tactcaaaac acgtgtaacg actaaaaatt
    42541 taaaaggatt tgtacctgaa acttccatag catcaatttg ctcagtaacc ttcatggaac
    42601 ctaattctgc cccaatactt gagccaattt tacctgcaca gattaaagca gttattaccg
    42661 gccctatttc ccttacaatt gaaatagcaa tcattgccgg cagccatgat gttgcaccaa
    42721 actcagctaa tgtgggtcgt gattgtactg taagaacaag acccataatg aatgcagtaa
    42781 tacctactaa tggtaacgac ttgtagccgt ttataaatgc ttgtttaaaa agttcttccc
    42841 actcatagcg tggcttaaat ccttctttga aaaaacgtat aacaaaagct gacagttgtc
    42901 ccgtttcatg taataaatcc gatatacctt taggcaactt cataaatcaa attccaagtt
    42961 gaaaaacatt ttcataatgc agctaagata attaataagt aattgcgaag ctattttaaa
    43021 gagaagggtt gagcgatgaa atctaattat atttcaaata gtagagtttt cttagcatct
    43081 ttgcattatg gctgaaaaag atagtatccc aaaaattcaa accgattccc atattgaagt
    43141 aaagcaggtt tatacatcgt gcaaaccaat gaacgaactg cctgcaaagt ttccatttac
    43201 acgtggtgta caggccgata tgtacagaag caaactatgg accatgcggc aatatgcagg
    43261 attttctact gcggaagaat ctaataaacg ttatcactat ctgctcaatc agggcacaac
    43321 cggtttgtca gtagcttttg atttgccaac acaaattggc tacgactcag accatgaact
    43381 ttcagaaggt gaagtaggaa agtccggagt ggctattgac tcattgcgcg acatggaagt
    43441 gttgtttgac ggtattcagt tgcagaacat cacaacatca atgaccatca attcaacagc
    43501 tgctgttttg ttgagtatgt acattgccct ggcaaaaaaa caaggtgcag acctttctaa
    43561 aatttctgga actatacaaa atgatattct gaaagagtat gcagcacgag gcacctacat
    43621 atatccgcct aagccaagca tgcgcatcat caccgatatt tttgatttct gcagtcgtga
    43681 agttccaaag tggaatacca tttctgtttc cggttatcat attcgcgaag caggaagtac
    43741 tgccgtgcag gaattggcat ttactttagc cgatggcaaa gcctacctca aagcagcgat
    43801 cgaagcagga ttggatataa atatttttgc gaaacgaatt tctttcttct tcaatgcaca
    43861 caataatttc tttgaagaga tagccaagtt tcgtgcagca cggagaatgt gggcagctat
    43921 caccaaagaa cttggtgcca ctgacccaaa ggctatgatg ctgcgttttc atacacaaac
    43981 cggaggcagt acattaacgg cacagcaacc acaaaacaat attatgcgnn nnnnnnnnnn
    44041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca agtgcagaag cttgtacaac tgcgctcaga
    44161 gagaaataat gaacaggtag gtcaggcatt aaacaacttg gaaacagccg ctaaagataa
    44221 taccaacctg atgcctttta ttattcagtg tgttgagcat tatgcaactc ttggcgaaat
    44281 ttctgatgca ttgaggaaag tgtttggcga gtatagataa tgttgtttaa ttaacagaat
    44341 ttgctggttg atgtgacaat tcgattctta gaaaagtgat ttttctcata aatcatattt
    44401 gccaatgtac taattttgca attgtgaaaa gagccatttt atttattctt acatctgctt
    44461 ttctttgtag tatattctgt caaagcttaa tttttgcgca ctattttttc aatagaacat
    44521 attacgctac ggtattgtgc gaaaataagt ccaagaagga aatgcattgt aatggtcatt
    44581 gtcgattagc aaaagaacta aaacagcaag aaaaatcggc agaaattcca tttacttcat
    44641 ttaaagagaa aaatgaaatt gtgcagtatt ttgaattaga agaacctttt agcttctctg
    44701 aattttttac cgatcaaatt ttcgtggttt ttaaaccatt tttagaacct gaaggatatt
    44761 ttcagaccat attccatcca ccagctgtat aatttattct tcaatttttt atttcgaaac
    44821 ctcagcaata ggctgatggc ttcttaagtt gaatacataa attattaatt atgagaaaaa
    44881 tagtatttat attattggct ctaataccat attatgcaca gtcatgtgac ttttgcaatg
    44941 gctatctagg gttaaatccg catttcaaaa agaacagtgt ttcaataaga aaccaaagta
    45001 ggttcttcaa gggaaccgaa atgtcggcaa cagaacttga tggcatggga ttatccaaaa
    45061 aagatttttt tgaaaccagg gttaactatg agctatttgc acaatattac cccacccaaa
    45121 aactacaact tattttatct gtaccctatg tatttaaccg tgatggtatg agtgataaag
    45181 cagcacatgc tatgggtgtg acacatcata atttaaacca tgaatcaaaa gaagccacaa
    45241 atacctatca aggtattggt gatgtagtgt tggtttcaca ctatcaagta ttcaatataa
    45301 actctacaga agaagataaa atcagtcaac gattgatggt gggtccgggt ataaaattac
    45361 ctaccggaat ttgtgatgtt cccgaaggta gcgaatcaca tacttttgta caccaacctg
    45421 gaacaggtag ctgggatcct atgataagta tgatatatct tgcgaagtac aagcgttcag
    45481 gtttaatggc aaacctatca tatatggtta caacaaaaaa caaacaaaac tttcgctctg
    45541 gaaataaact aaatactaat gttacaggtt tttatcagat tacttctaaa aggtttaaca
    45601 tctatcctaa catcggattt tactgggagt atgcagccaa agatatttac ttgagtgaaa
    45661 aacaaggaaa cagtggtggt aacatttctt acctgcataa cggaatagac atctattata
    45721 aacgatttgc tctgaatgct gcgtttcagc taccaatact caaaacactc aatggaaatc
    45781 aggcagaaat ttcttttaaa gtactaacag gcttttcttt tacattaaac taaaaaaata
    45841 acgaataata aaataaaaaa atgaaaaaaa atacaacaat aattgctgta attgcaattg
    45901 caataatagg gttttgcatt tccggatgta aaaaagataa agacaacgaa ccaactgagc
    45961 gagagctgtc ttttcatcta cacaccttgg ttggaaacac gattgctaac tataatgatg
    46021 ttttttctga caatacagga agaaaattta aaatagatga ttgccgattc tatatcagca
    46081 acattgtact cattaaagat gacggaacag attatccact gacaaatgtg gtgctgctgg
    46141 ccaaccctaa cgttcaggag tacaacctcg gaaaagtacc agtaggaaag tacaaaggat
    46201 ttcgttttct gatgggatta gattctgtta ccaatcatgc tgatcctgcc tcctatcctt
    46261 caactaatcc tttgagctat cagaacccaa caatacactg gggttggaac aatggctaca
    46321 tcttcatgga gtttcaagga aaattcgact ccacttttgc tgccaatggc acaccggatg
    46381 ccgatttctt ctaccatgta ggcatggata acctaaaacg tactattgat tttagtgcat
    46441 cagcctttga agtttcttca aatagcgatt acgaaatagg cattcttttc gacctgaaaa
    46501 aagttctcaa taatgtggat atgcgcacag agtttggtac acatacaatg gataatatgc
    46561 cactggctgt aaaaattgcc aacaactggc aaggtgctta cagcattgaa taatttgcta
    46621 tcttcggggt agaaaatttt atatcatgaa tattttgaga ttcgtatttt ttgctgctgt
    46681 tgcaggtttg attttctctc ttagctcttg caaaaaagat gattcgtccg acaatcaaat
    46741 tccgaacaca gatactatcg gagaacttag ctttaactta aagcaatatg ttggcaacaa
    46801 tagaattgct tacaatcagg tgtacagtga tacctccgga agacagttta aaattaccaa
    46861 cctgcgctat tatatttcaa atattgtttt attaaaagca aacggtcagg agttgcccct
    46921 tagtggaaaa gtgcttttgg ttgacaatga aacagaacaa tattcattgg gtgaaattcc
    46981 tgtaggagat tattcgggtt tgcgatttat ggttggtatt gattctgcta ccaatcacct
    47041 tgaccctgca ttgtatgatg cttctaatcc gttaagctat cagcctgtaa gcatgcattg
    47101 gggttggaac tttggctata tttttatgaa ggttgaaggc aatgtagata cagatactat
    47161 tcctaccgga aatttaaatg gaattatttc gtatgatatt ggaagtgatc caatggtacg
    47221 ggtgataaat tttaatcata ttttttctgt aaatggcaat accaatacca taaacattaa
    47281 atttaatctg cttaaaatgc taagcggtat agatttggca acagaaagta ttacgcacac
    47341 caatccgggc agggatttag cagaaaggat agcaaacaac tggcttattt ctttcaccat
    47401 tgaaccatga ttaaaaaaca gaagtctaaa ttttttatta tttcaatttt tgttttttgg
    47461 ataataggct ttacagcttg tcgtaaagat gatgccattg acacaggaaa tgaggtgctc
    47521 gaactgcaag taccattagg atttccatat cctcccatac ctgaagataa tcaacctaca
    47581 aaaaacagaa ttgcattagg caagatgttg ttttttgatc ccatactatc acgtgacagt
    47641 agtatttcat gttcaagctg tcatcatgcc gataaaaaat ttacagatgg acttgctgtt
    47701 gcagagggca ttgacggacg aaaaactttg cgtaattcaa tgaccatact caacgctgca
    47761 tatcaaccca atatgttttg ggatggaggt tcaccgacat tagaaacaca agtgtctgca
    47821 cccatagaag cgcatgaaga gatggatttt aatgtgatga aagttgttga acgtttaaat
    47881 aatcatgctg tatatcctga cttgtttaga aaagcatata acagtggccc atctgcattt
    47941 acgcttacaa gagccattgc aaattttgaa cgttcattgt ttacaggaaa atcaaagtat
    48001 gatgagtatc agtatgagaa aaaaagtgat gctttgtcag cttcagaaaa aagaggtatg
    48061 gatttgtttt ttggtgagcg ggcagagtgc ttccattgtc atggtggatt taattttact
    48121 gattttagct ttaagaacaa cggactttac gaagtgtatg cagattcggg cagggctcgt
    48181 ataacaacct tgcatgatga tgtgggagaa tttaaagtgc catcacttcg caatgtggca
    48241 ctgacagcac cctatatgca cgatggcagc atgattacgc ttgaagaagt tgtggaacat
    48301 tataattcag ggggcaaacc aaattttaat aaaagtttcc tcatcagacc attgaacctt
    48361 actgcacagg aaaaagaaga cctggtaaat tttctaaaag tattaacaga tcaataattt
    48421 atttgaaatg aaaaacatct ctaaactcat cgcattaagt tttcttttat tctccattgt
    48481 atcatgcgaa aaagatgatg gctgtgatgg aggaacagga ggaaaaaatt ctttaaaagt
    48541 ttatttaaag catcattcaa aaattattcc gccacaatca aactatccgg atacactttt
    48601 tattaagtac ggcagtagcg atcttccggg taccgctctc tctgactttg ataactatgt
    48661 cgtaaacagc gatacttctg ttgacttcct gaccgttcca aacttaaaat gcggagatta
    48721 ttacctgtat ggtgtaggtt gggatgcagc tatttctcag cgtgtaaaag gaggagtgcc
    48781 ggtaaccatt cctgacaatg atgcagaggt gactgttaat gtaccggtaa cagaagacta
    48841 agacgtattt ttttaagaaa gatatttccc tacaatgggc atgcgtcttc ctaagccaaa
    48901 tgctttagaa gaaatccgca atacaggtga taattgtttg cgtttatatt catttcggtt
    48961 aaccatactc aaaattcggt taaccattgt ttcatcatgt ccacctttaa taatttctct
    49021 agggccttta cgcagttcaa tatattcaaa taaaatttta tcgagtatat cgtaaggtgg
    49081 caaagagtca ctatcttttt gtcccgggcg caattctgca cttggtgcct tacttaaaat
    49141 attttcagga atgatttcct tatctcggtt gatataattg cacagttcat aaacttctgt
    49201 tttatacaaa tcaccaatca cgctcagccc gccacacatg tcgccataca aagttccata
    49261 gccaacggca agttcacttt tattacttgt gtttaaaagt atcgggccaa atttattact
    49321 cagtgccatc agcaaggttc cacgtatgcg tgcctgaatg ttttcttctg taacatcaaa
    49381 aggcttatca ccaaataaag gtttaagtgt tttcaaaaat gtttcataca atggatttat
    49441 ttccatttta tcaactttga tgttaagatt ttgacaaagt tgttcagagt ctgagattga
    49501 atgtccgctt gaaaaagcag aaggcaacat aacaggcaaa atattttctg cacccaaagc
    49561 atcggcagcc aacactgtaa caatggcact gtcaacaccg cctgacaaac ccagtatggc
    49621 ttttttaaat cccatcttag aaaaataatc tcttatcccc atcaataatg cctgatagat
    49681 caattcatat ttttcggttt gcttttcatc cacaggcgaa gtgtttgctg tattcagata
    49741 aataatttct tctttaaagt aagggccttc atgcaccaat ctgcctttgg catcataggc
    49801 taatgaaccg ccatcaaaaa taatttcggt ttgtgctcca acatggttga catatagcaa
    49861 tggaatttta tattgattta catttcgctg taaaattgct ttccgctgat gcgcatgttt
    49921 gtaatcaaat ggcgaagctg caatgttgat gatgaaagaa ggtttttctt ttatcaactc
    49981 ttccattgga gaatgtatgt agagtggatc gtcttcaaca ttccacaaat cttcgcaaat
    50041 ggttaaagca attttttctc cgttgagttc aatacattga aatattctgc cggattcgaa
    50101 atagcggtac tcatcaaaaa catcgtaagt tggtaataat gctttatgaa taactgactg
    50161 caccttacct tgatgaataa accaagctga gttaaacaag ctttttcctt cggggacggc
    50221 attgcgcgat gggcaaccta caataactgc aatattctga ctgtgtgctg caattttttc
    50281 aatgctgtta taacagcggt tgatgaaatc atcaaactcc agaaagtcaa gtggagggta
    50341 tgccgtaatt gcaagttcag gaaatacaat cagtgctgca ccggattttt cggcttgttt
    50401 gattgcctca ataatttttg tggtgttgtt ctcaaagttg cccacatgaa agttgagttg
    50461 ggcaagtgca atgcgcatat ttagaaatta tcaggcaaag gtatatttat atgaataatg
    50521 ttgccttgtc tttcttaata atctttttct aaagtaatct aatcttcaag gtatgacata
    50581 gctcaaatat aaaaagctac cctttgaggt agctttttat aaaatttcta tttattactt
    50641 aaaacatcac gcccaaatca acagctaaaa aatttgatgt agcttttgaa tgtttttctt
    50701 ttactacatc aagaaaacca ttattgaaag ttatacctaa aagtaaagct gtttttccgc
    50761 ctaatgaata ttgcgttcca ataccggcta ctaaggccaa attcaatggg ttaatgtcag
    50821 actttttaaa atctccatct gttctgatgt taacacctgt agtaaatcca actttaccaa
    50881 agtaagtcat ataaccaatt tcattggttt tcaactttaa ggttaaagga agttcaatat
    50941 attgtaatac tgttttcatt ttataagtat ccagatattt caatttgccg ccacggtatg
    51001 ttacatctat tccggtttca aaggaatagt tgttggtaaa attaatatcg gcaatcagtc
    51061 cataggcaaa ccctatttta ctgccatgat tatctaaatt atcactggcc ttaaaccagg
    51121 ctactgttgg cgaaatttta agtccgaaat taaatttgtt gtcctgcgct tttacaaatt
    51181 gaaaagcagc tacaaaggct attgcaatta gtgatacttt tgctctcatt attttatgtt
    51241 ttttaattcg agacaaaatt aatgaaattt ttcacagtca gacatctatt ttacttttta
    51301 ctatcaactg tgctgatggt ggttttttcc tgtcagaatg acccacatgg agataaacat
    51361 gctgcagaag caataaccac acatttgaaa tttgatgaag tgctgttttc tgctgacagc
    51421 attagcccac agcatgtggt gcaatgggaa caacattatc cttatttttt taatttgttt
    51481 gttaatagaa taatacatct gctaccaaca gatagtgctg cacttgcatt aaatctccaa
    51541 aaatttgttg gtgataaaga tgtaaaagac attcatgcac gggtagaaaa agttttttct
    51601 tctgccgata aagataaatt gttttctgaa atagaatttt ttttaagcaa ctataaaaat
    51661 gagttaggca cacaacctgt aaagcatctg gtaacttttc tttctgcatt taactatgct
    51721 gttataaccg ctgacagtac tattggcatt ggtctcgaca tgtatttggg cagcgattgt
    51781 gaattctatc cttctattgg aattccacag tatgcttatc agcgtttttc taaagactat
    51841 attttaagtg atgtcattaa agggtggtat cagagtgaac atgatattga tgcagagaaa
    51901 aatgatttcc tcagccgcat gatttattat ggcaagcaac tgtattttac ttcagttatg
    51961 gtgccacaaa tgcaagactc tattctgaca ggatacagta aaaagcaatt ggagtggtgc
    52021 aacaacaaca gcaagcagat gtgggcattt tttatagaga acaaactttt ttattctacc
    52081 cgcgaaatgg aatacatgaa atacattgca gatggtcatg ccacacaagg ttttcctgaa
    52141 ggcgcaccat cacgtacagc acagtggctg ggatggaaaa ttgtaagcgc ctatatgaag
    52201 aacaacagtg tgaaattaaa agagttgcta gcagaaaaag atgcacagaa aattctggag
    52261 aaatcaggat ataaacctta gttcaaagta aaaataacaa caatcggaga gatgaaaaaa
    52321 tcagaaataa aatttacagt cagcttagac gataacaaca agcctgtggc cttgcattgg
    52381 aatgccgaag acagtggcat gcagggcgac aaagagtgta aggccatgat gctggcattg
    52441 tttgataaga aagatcaaac caccatgcgc attgatttat ggacacagga aatgatggtt
    52501 gaagagatgc aacaattctt ttttgaaatg tatatgagca tggccgatac ctatcaaaac
    52561 gccacaaacg acaaagatca ggcagaaaaa atgcgtggct ttgcccgtca gtttggtaag
    52621 gaagtgagtt tgataaaata actatttttt attgatagta gcaacaagaa cagttcttga
    52681 aaataacctt gttgattttc ataattgata aaatttgttt cgcttgattt tgacttctat
    52741 agataataaa caaataagat gaaaaagcta attgtaattg caggtgtgat gcttcttttg
    52801 ttaatagcaa gaaaaaaaac agaagcagac aagagcattt tctggtacat tcagatgcct
    52861 atctaaaagc cacaaacaat aaagatcagg cagagaaaat gcatggtttt gcctgtcagg
    52921 ttgataaaac agtaagtcaa taataaataa aaggaaagta aattatcctt tattggtaat
    52981 cttttgggca aaagcaatga tatgaccatc agggtcggaa aagtaacaag ctctgtcgcc
    53041 ccaatcccta tcaataattg gactaatcaa atgtgcacct gcatttattg cattgtcaaa
    53101 ctcaagttca atgttttcag cataataata aagttcgcat ctgggtattc cattgcctag
    53161 atttggatga ggggttttat ctgaaagaat ttttgctata ccataatttg gcatcaatcc
    53221 aagtttacaa ttaggggcaa gtataaactc tgtcatgccc ggcacattta agtcaggctc
    53281 ttgtctgaat agcttggtat aaaaaactgc actttcctgc tggttcttaa cataaagaat
    53341 ggtttcaaag agtaaaaagt ggttcatcat gctatgcttt tttcttctgt tgagtgattg
    53401 tgtcgtatag tattttggtt tgagaaaatg tgtctaagta ttaataccat tttttggtac
    53461 tagtctgact ttttttgacg gagttttttt atttcagagg ttcttcgttt ttgagtcaat
    53521 cggctttctg ttgcctgtaa tggttttgat gtggctattc gcttgacagg ctttttcaaa
    53581 gatttttcaa tcagcgtttt gaattttttc aataccagtt ctttgttgcc caactgactg
    53641 cgtgcttcgc tgctttgtag ttttatgaaa ccctcatcgt tgattcttcg gtatagcttt
    53701 tctgaaatga tctttttttg ctcttcatct aaacatgcag agctatctat attaaaggca
    53761 agctcagcct tagtagaaac cttgtttaca ttttgcccac cttttcctga actgcgcgag
    53821 tatgtcaata ccacttctga taacagacat ttgaaatcta ttttatccat tacaatttaa
    53881 tgctaaaact atcagcatcg ttgccaacag gaaaaatgtg tcttgcattt tccaaaacat
    53941 caaagtcgag ttttatagtt tctatatttt ctgtatcaga ttttgttttg ctgatttttt
    54001 ttccaaatgg atccagcact acactgtcac cgctataggt aatgccattg ccatcttttc
    54061 caatacgatt aacaccaaca acaaacgatt ggttttctat ggcgcgtgcg gttaacaatt
    54121 gtttccaggc aaagctgcgt ttctcaggcc agttggcaac aacaatggca caatcatatt
    54181 cattttggtt gcgcatccaa acaggaaacc gcaggtcgta acaaacaaaa accgaaagcc
    54241 gccatccttt gtaaagcaga atcaattttt tgtttcccgg atgatagaat ttttgctcgc
    54301 ctgccatggt aaacaaatgt cttttgtcgt agtgtgcatg agagccaccg ggagtcatcc
    54361 atatcaaacg gttatagaag cgtccatttt cttcaatgat gaggcttccg caaacaacag
    54421 cattttttgt ttctgccatt ttttgcatcc attgcatggt agggccatca cttttttctg
    54481 ccagtgcatt tgcattcatg ctaaagcccg tgctaaacat ttcgggcagc acaataagat
    54541 cggtgctacc tgtctttatg cttttaagca gtgtagtaaa atgtttaatg ttttttacct
    54601 gactttccca gtgcagcttt gtctgaacgg ttgttactct tagagtactc atggtatgat
    54661 gttgttggtt caggcaaagt tatattttaa aattgcagaa aagggattat ttttcaacct
    54721 tatgcaatct ttcagcagct ttttcaagtg tttcttcaga tttggcaaaa caaaacctta
    54781 aaagtttatg gtctgtaccg tttctgtaaa aagccgaaac cggaatggat gcaactccat
    54841 agtcttttgt aagctttaca gcatagtcca tatctttttc acggctcagt ttactgtaat
    54901 cgagtaattg aaagtaagaa cccgaacagg ccaatggctt aaatgcagta ccttgaatca
    54961 gctttaaaaa gtagtctctt ttctcctgat aaaatttttc aatacctgaa tagtttttgg
    55021 catctttcat aaattctgca taggcatatt gaatgggtgt gttggcacta aaaaccataa
    55081 actgatgtgc ctttctgaat tctgccgaaa gctttgcagg tgccagacag taacccattt
    55141 tccaccctgt ggtatgaaaa gtttttccga aagaataaac aataaaacta cgctcggcta
    55201 gttttggaaa gcgcgcaaca ctctgatgtt cgtagccatc aaaaataaca tgctcatata
    55261 cttcatcact caagacaaaa atgtctgtct ttgctgtgat tttttcgagc atctgaaaat
    55321 ctttaacact taagatagca cctgtaggat tgtgcggtgt gttaatcata atcattctgg
    55381 tgcgctggtt catgagtttt ttcacttcat tccagtcaat atgaaagtct ggcattttta
    55441 actctgcatg aacaggttcg ccaccggcaa gaacaacagc aggtgcatag gaatcgtatg
    55501 caggatcaaa aataattact tcatcgcctt cgcgtactac agatgctatg gcagtgtata
    55561 tagcctgtgt tccacctgca gtgatagtaa tttctgtttc aggattatat tttgcactaa
    55621 aacgtgcttc gattttagca gcaatttctt ctcgcagcgg catgataccc ggcatgggtg
    55681 catattgatt catgcccttt ttcatgtact tagtcactaa actgatgagt tcaggcgaaa
    55741 tggcaaagtc aggaaaaccc tgagaaagat taatggcatt acattcgttg gcaagtgccg
    55801 acatgacact gaaaatactg tttccggttt taggaagttt cgattttatt aatgcttgat
    55861 ttgctgacat aaaaagcggc tatgcttcaa atgtaaatca attgccgata acaaaaaaga
    55921 aatctgcttt tttagtttat gtttatatgc tattgcattg agtataacag taaattgttt
    55981 taaacaacat tcgcttggac ttctttttat ttatgttttt gacagttgta gaattgaagt
    56041 cttttgttta aagttagccg ttttgaaaat cttcattgat atgaattcaa ttatatttgt
    56101 tagctcagtt aatgtttagc attttgattg aatgaaattt ccatcaatta atttttcgat
    56161 ttatggcaaa agaacttatt tggttcttat cagtgtattg attggtgcat taactacttt
    56221 atgtgcagcc attttaaaaa aaggcgtaat gctgatggaa catattgcag agcatttggt
    56281 ttttgattca ggctatcatc agttgttttt aattttaccg gcaacaggaa tattgcttac
    56341 agttacattc tggaaactga tgaaaatcaa gaatgaaaga aggggaatga cggcagtaat
    56401 ttataacatc tccaaacagt cgagtgtagt tgccaagcat aaactgtttt cgcaaatggt
    56461 tagcagtatt tttactgttg gttttggtgg ctctgcaggt cttgaagctc ctattgcatc
    56521 taccggatca gcaattggaa gtactattgc aagcaaatta aaattctcta ccaaagaaag
    56581 aacactatta ctggcctgtg gtgcagcggc aggaatttct gctatcttta atgcacctat
    56641 tgcaggtgtg gtgtttgcac tcgagcttct gctcattgat atgcctgtgc ctgtcgtcat
    56701 acccttgttg atatcaagtg ctactgctgc atttttatcg cgctatttta atctcggtca
    56761 gccttttgtt ctcataaccg acacatggaa ttcaagtcac atactctatt atctcgcttt
    56821 agctgcattg gctgccatac tttctatcta ctgtattaaa atatattttt tcatcagctc
    56881 aatattttca aaaatctcca atgtatttgt aaaagcactt attggcggaa ccttactggg
    56941 gttgctgata tttttatttc ctccacttta tggtgaagga tacgacagcg ttcaattgtt
    57001 attacacagc caatccggtg aaatttttaa gaacataacc ttttttgaag ccaatgcctt
    57061 gtggctacca atacttgcag cactactgat gttttttctt aaagtagtag caacatcgtt
    57121 tacggttttt ggtggaggta atgggggtat gtttgggtca tcattattta caggtgctat
    57181 gctgggtttt actttttcga gagtagtaaa tcttatgggc tgggcagagt tgaatgaaat
    57241 aaattttact gtaataggaa tggcatcatt acttagtggt gtacttcaaa caccgctcac
    57301 tgcaattttt ctgattgcag aaattacagg aggctatgcc ttatttgttc cgttgatgat
    57361 tacaacatca ataacctatt tggctactcg atatgtagaa ccacactctg tttataccaa
    57421 acaattggca cttaaaggcg gactaatcaa tttaagtaaa gacaagcagg taatcagcaa
    57481 aatgaaatta agagattatc ttgaaaccga tttggaaaca attaaaataa atgcaactct
    57541 tggtgattta attaaggcta ttcgtaaaag taaacgcaat atttttcctg tggttgatgc
    57601 catgaatcgt ttacagggaa ttattttact tgatgatgta aagcgtgtca tgtttgatca
    57661 gtcgcagtat ggcacaggtg tcaaagaact gatgcaaaga ccaccgttgg ttttggacat
    57721 tgaggaagat atggaacttg ttttagaaaa atttgaagag tatgaagcct ggaattttcc
    57781 agttactgaa aaaggggttt atgcaggttt tgtttctaag tctaagatat ttactttcta
    57841 caggcagctt cttattcagg agtctgacgt gcctatgcac gattaacgac agaattaaaa
    57901 gtaaaaaagc tcctttgcag gagctttttt ataattttca gaagattatt ttttcttctt
    57961 agttgctgct tttttcttag tagctgcttt cttttttgga gcagctttct tctttggagc
    58021 tgctttcttt ttagtagctg ccttcttttt tggagccgct ttcttttttg gagctgcctt
    58081 cttagcagaa actacttttt tcttagtagc tgcttttttc ttagtagctt tctttttagt
    58141 agccgctttt ttcttagtgg ctttcttttt tggagctgct tttttcttag ttgcagcttt
    58201 ctttttagta gctgcttttt tcttagttgc agctttcttt tttggagcag cttttttagc
    58261 ttctaccatt tcagcacctg tagtgctttc aatcattgaa ttgttcatca tgatgattat
    58321 ttatttattg gttattgata ttgttatact cagagtttgc tgataagtta cacttttagt
    58381 ttacatcaac aattttctct gccttatatt caccgtttaa taatttcgat tttatttttc
    58441 cgaaagattc aattgtatat tttacatctt ctaatgtatg aactgctgta ggtattaacc
    58501 taagcataat aattcctttt ggtactacag gatacacaac cattgaacag aatatgtcat
    58561 agttctcgcg taagtcgtaa ataagtttag tagcttcagg aatagttccg ttaagataaa
    58621 ccggtgtcac cggtgattgt gtttttccaa tatcaaatcc tgcttgcttg agtccgtttt
    58681 gcagggcatt aacaattttc caaagattgt ttttcaactg tggttgattt tttagtagct
    58741 caagtctttt aatagcaccc tctactaaag gcattggcag gcttttcgca aaaatctgcg
    58801 aacgcatgtt gtatttaaaa tattctataa tctgcgcatc gctggcaata aatccgccaa
    58861 tgagtgccat agattttgca aaagttccaa aataaatatc tacaccatcc tgaactcctt
    58921 gctcttcacc tgttccggca cctgtttttc ccatggtacc aaaaccgtgg gcatcgtcta
    58981 caaataatct gaagctgaac ttgcttttca gggctactat ttcttttaac ttaccctgat
    59041 caccactcat accaaaaaca ccttctgtaa taactaatat agcaccaccg gtttcttctg
    59101 caagtttggt agcacgctca agttgcttta caaggttgtt aatgtcgtta tgcggatata
    59161 caaaacgttt tcccatgtgc aggcgaacac catcaataat gcaggcatgc gattcggagt
    59221 catatactat tatatcatgg cggtcaacca aacagtcaat ggcactaacc attccctgat
    59281 atccgtagtt taataatata gcgtcttctt tttgttcgaa agcggcaagt tgttgttcta
    59341 ataatgtatg tctgtcggtt tcaccactca tcatgcgcgc gcccattggt gttcctaacc
    59401 catagtgtac cgatgcttcg ccatcaattt tgcgaacttc aggatgattg gccaatccca
    59461 gataattatt taaactccaa accaactttt cttttccgcg aaaaatcatt ctgttcgaaa
    59521 tctcaccttc aagttttgga aaagtaaaat agccgtgcga ttctttggca tgcataccta
    59581 acgggccacg gttcttacta atcttgtcaa acaaatctgt cataaaaatg ctaaaaaaaa
    59641 aattcgcttt tgctttttaa aattgtttcc gaaatatcac cacaatttta tttttgataa
    59701 ccatgctact taatattttt caacagaaaa ttgttaactg cttttttgaa gataagaaaa
    59761 tattttttga aaaaaaagta atcgcagaaa aaaaataaaa aactttttgt ttcaattttt
    59821 cagaggagga aataaatgtt gttgttgtaa ttattaaaaa aaatattttt aataagcatt
    59881 gattttttat aatgatacac aaaaagaaat ggagtgtgtt tgttaatatg tcaacaacct
    59941 gagtgtaaaa aaaattctta aaacggtaat tgtgtgcaaa agttttgatc aaaatctgtg
    60001 ttaatgcaaa caaaaaaaca attctgtttt tgggttgatg agtaaaaaaa attacaatta
    60061 attttttaag aaactcaata ttgtttttag gttaatgatt attgaaagtg tgtttcagga
    60121 tagttctaaa aaccgatgta atgtatttat aaaacaaaaa agccgcttcg gttatcggaa
    60181 gcggccttta gttatctgtt ctttaagaac ctctttgttt ctctttgtgt ttggtgattt
    60241 gtcgttttag tgcttcaacc gatttatcag ctgcctgctc aaaggtggca cattgctcac
    60301 ttgcaaaaag tactttaccg ggtgtgctga ttttaatttc agctatttta ttttcctgcg
    60361 ttacgttttt gtctaaacgt aaaaatactt caccatctat tatgtgctca taaaactgtt
    60421 ttagtttgtc aaccttttct tctacaaaac gaatcaggtt tttgtctgca tcaaaatgga
    60481 tggactgcac tttaatcttc atactctatt ttgtttttag tacaaaaccc acacattgcc
    60541 cttttgctgg tgttttgtgg tttaaaatat tctttgggca tggaaatatt ctgttttgaa
    60601 aagtaaatat acgaaatcaa gccttaggat gtgcttgttt gtagatattt ttcagcttat
    60661 caatggtgtt gtgtgtatag acctgagtag cagcaaggct gctatgtccc agaatttctt
    60721 tgatggcatt tatttgtgca ccgctgttga gcatttctgt tgcaaaagtg tggcgcaaaa
    60781 cgtgcgggct tctttttttg ttggtggtca cctgactgag cattacatta actaccttat
    60841 aaacgaattt ggggtatagt ttgttaccat catttttaat aaaaaagtac tccgacttga
    60901 ttttttcatc aatgacgttg tttcgttcat ttaaatatac aactatagcc tctttcattt
    60961 ccggtgtgat gggaatgatg cgctctttat ttcttttacc caatactttt aaagtcagat
    61021 tgtagaggtc aacgttttta tctttaagtc caatcaattc ggcaagacgc atgcctgtct
    61081 gataaaacat atttataata agcttgtcgc gaataccttc aaacccttct ttaaatgcaa
    61141 caccatccag taactgatcc attttttggc ggtcaacgat tacaggaagt tttttggcag
    61201 tttttggcga aacaattttc agcattggat tttggctgat ggttttttcg cgcatcaaaa
    61261 atttgaaata agttttcagt gaggagattt tccggttaac ggttcgtgaa tctattttcc
    61321 ggtctaataa agaaactatc caactgcgaa taaaaaagtg agtaatctct tcgggcttct
    61381 gtgcctgata ggtctggccc ataaaatcaa aaaactgctt aaggtcggta ccataagcag
    61441 ttaaagtatg ttgcgaaaat cgtttttcgt attgaagaaa tcgcagaaac tttgcgtaag
    61501 gcataatatt gtttatgcca aatataaaag catattcaat aggtgcagtt caaaacaagg
    61561 cttagaaaag atgaattata aaaagccctt tgttaagaat tacattgcgc cttcggcctc
    61621 taagcgctga agttttgtgc ggtgtattgc ttttttaact tctgttctgc gggcaactga
    61681 tggtttttca tatgcctgac gtgaacgcag ttgttttaca acacctgttt tttcaaactt
    61741 ctttttaaac ttctttaaag ccttatccaa aggctcattg tctttaattt gaacaataat
    61801 catattataa tttcaaaatg ttttgggagc gcaaaggtaa gtaaataatt ggcataataa
    61861 tcaatacaat agaaattttt taagaaagta ctaccctgcc tttatctgac cttaactgtt
    61921 gattattaat gtgatggatt agacttcttt tcaggtttta tgttaaaacc atagtgaatc
    61981 acattaaatc tctaccaata tctttcctat aaaaggcctt ttcgtaggta atttcagcca
    62041 attgttcata gactttttcg cgggcttctg ccagattttt acccaatgca tttactgcaa
    62101 gtacacgtcc gccactagtt agtactttgt catcctgcaa gattgttccg gcatgaaata
    62161 catttacccc tttaactttt tcaagtcctg aaatgatttt tcctttttca taagtttcag
    62221 ggtaaccacc cgaaaccatc ataacagtaa aagaggttct gtcatctgtt ttgagttcaa
    62281 cttcgtgaag ttttttttgt gctacactat tcatcaactc aataaaatca gtttttattc
    62341 ttggaaaaac agcttctgtt tccgggtcgc ccatgcggca gttgtattca ataacaaagg
    62401 gattgccatt tacattcatc agtccgataa ataaaaaacc ggtgtatgaa atattttcat
    62461 gctttaatcc atttatggtt ggcttgataa ttttgtcttc taccttttgc ataaatgcag
    62521 cttctgcaaa cggaacaggt gaaacagatc ccataccacc tgtattaagt ccggtatcat
    62581 tgtcgccaat tcttttataa tctttagctt caggaagcag acaataattg ttgccgtctg
    62641 tcagaacaaa aacagatagc tctatcccat tcagaaattc ttctatcact actttgtttc
    62701 ctgccttgcc aaaaggttgg ttgatgagaa gatgatgcaa ttgttccctt gcctcagttt
    62761 ctgtttcgca aataataaca cctttaccgg cagctaaacc atcggcttta agtacgtagg
    62821 gtgcagaaag tgtattcaga aaattataac cttgctgtag ttgcgatgat tcaaaagttt
    62881 catatcgtgc agtaggtata ttattccgta gcataaaagc tttagaaaaa tctttactgc
    62941 cttcaagtct ggcacctgaa cgttcaggac caattacagg aacagatttt aactcgctat
    63001 caccaagaaa aaaatcatga atgccattaa ccaaaggttc ttctggacct acaatcacca
    63061 tgtcaatttt cttttcaagc acagtttttt tgatgtggtc aaaatcattc acactacccg
    63121 aaatgtttgt tcccaactgc aaagttcctg cattgcccgg cataatatat aagtgtttca
    63181 gcaatgcact ctcactcaat ttctgtgcaa atgcatgttc gcgcccacct gatccaagca
    63241 gtaaaatatt catgctaaat ttctttctgc aatgataaat aaatatggtg taatgtaacg
    63301 tttgttaatt ggctgccttt ttattgtgct gaaaggacat attccatatt aaaacccgaa
    63361 gctgccaata actgattttt ttactttatt tttatgccga agaaatttaa gcaattactt
    63421 ttttataaaa gcaaaataaa catttgatga gcagcctttt taataagcaa gatggcgaaa
    63481 aaataattga acgcatagca acacttcaat cggggcaaaa acctaattgg ggtactatgt
    63541 cggtggagca aatgttgctg cattgccagc aaccatttaa agtggcgttt gaagagttga
    63601 aacttaaacg cagcctggtg gctaaacttt ttggagggat agcaaaaaaa tcggtcatga
    63661 gtaataaacc atttaaagaa aacctgccta cggcaacaga atttaaaacc aatcacctga
    63721 atccgcaatt tgaaacagaa aagaaaaacc tgattgcctg tattcagaaa tttcagtctg
    63781 ccggacccga aggtataacg cagcagccac atcctttttt tggtaaaatg acagacaaag
    63841 agtgggatac gcttcagtac aaacacctcg atcatcattt aagacaattt ggcgcatgaa
    63901 attaaggttc atacctgtgg tttttataat atctttgctt atgatgtcgt gcgaaaatga
    63961 tatagagcaa gtgaaattag taacagggaa tgttgttgaa cctattgaaa gtgccaaagg
    64021 acttgaaatt ttatacagcg actcgggcaa agtaaaagtg cgcatatcag ccggagaaat
    64081 gaatcgttat ttgcaaccca agccaatcac agaaatgcct aaaggtgtta agattgattt
    64141 ttttgatgat aacctgaagg taacttccac actgacaagt gactatgcct tgcgtgacga
    64201 ttttgataaa aacatggagg caaaaaagaa cgtagaggtg gttaatgtta aaggagaaaa
    64261 gttaatgact gaatatttaa aatgggatga aaaaaccgga ctgatttcct caccagaatt
    64321 tgtaaagata attactgccg atgaaattat ttatggcaac ggctttgagt caaatcagga
    64381 tttcagccgc tacaaaatat ttaatatcaa aggtattatt aacctgaaga aaaatgaaaa
    64441 tactgagaat acttgagata tcatggctga taatagccat tggttcatta tgtgccggaa
    64501 tctacaaact gatttcagca ggactaaacc aagctgtatt tatttttgca atttctatta
    64561 ttgcatttgt tttttttatg ataagacgaa aacaaagaat ttcaatggag aaacagaatg
    64621 catcacgata attacttttg catcactaat ttcaactaaa ttctattaat gatttccgac
    64681 tttcttcctg ccatagttgc atcacttgct tttgttgcat tttattcagg aatagaaatt
    64741 gcctaccttt caagcaacaa atttaaaata gagctcgaca gcaagcaagg caaatggagt
    64801 ggcaaaattt tatcctattt cagcaaatcg ccttcacgtt ttatctgcac catacttgta
    64861 ggtcttaata tttcattggt aatttatggt accaacatga gtggtttgct caacccctat
    64921 ttgcaaaagt ggttgccaca gcaacttaat agtgaaattg gtcgtttgat aatcgaaaca
    64981 ttaataacca ctttctttct gcttttggca ggagagtttt tgcctaaaat attattcaac
    65041 ataaacccaa acgatacttt aaattttttt agcgggttta tttggctcag ttatattatt
    65101 cttttcccat tagtttggtt aattttaaaa cttgcccact ttctgttgcg caatgttttt
    65161 aaagtagatt ttatagaaga ggcaacaggc tttggtcgtg ttgacctgga taattttatt
    65221 gaagaaatta gtgaaaattc aggtggcaag agcgaataca ataaagaaat tcagatgttt
    65281 cagaatgcac tcgattttga tagtttgaaa gtgcgtcagt gcatgatacc acgcaatgaa
    65341 attatttccc tcagtagtaa ggagtcggta gaaacgttaa gaaagaagtt tgttgaaaca
    65401 ggactttcca aaataatgat ttatcaggac acaaaagata acattgttgg atttgtgcat
    65461 agctatgaaa tgtttaagaa tcccgagagc attatgtcag tgctgctgcc tgtatccatt
    65521 tttccggaga cattgcccgc acgtgagttg cttacacatc ttaccacaaa ccatcgcagt
    65581 gttgccattg ttgttgatga atacggtact acttcaggca tggtaaccat tgaagatatt
    65641 attgaagaaa tttttggtga aattgatgat gagcatgaca ttgatgttta tctggaacag
    65701 aaaatttctg aaacagaatt tttatttgcc ggacgccttg aggttgacta tctgaatcag
    65761 aaatacaatc ttatgcttcc ggaatcagaa ttgtttacca ccttagctgg gcttattttt
    65821 aatcatcttg aagatattcc gaatgtgaaa gagcagttta ccattggcaa ttataaacta
    65881 actgttgaac aggtaagtgg aaataaaata gagcaggtaa gactgataat tttagataat
    65941 accgaaacct aaatttcctt ttaataggct cttgtattga ttccctgcat gtagttaatg
    66001 aaagctttgc tcaccacctt attaccaccg ggtgttggat agtttcctgt aaaataccaa
    66061 tcacctttat tatacggaca agctgtatgc aggtcttcta ttttctgaaa aattatttct
    66121 acatcagctt tgcagtcttc gggttttagc atagaagtga tttttgtcga aagttgttca
    66181 ggtgaaaaag gtttatataa atcacgcaca taatttttca tttcgtttac acccaattca
    66241 agttgttgct tgcaatgcat gtaggtttct tcaataatat gttccatatt attttcttca
    66301 agcaatgcaa tagctgcttt gaaagcacac aggtcaccga tttttgccat gtcaatacca
    66361 taacaatcag gatagcgtat ctgtggtgct gatgaaacaa taattatttt ctttggacct
    66421 aaacgatcaa gcattctgat gatagaattt tttaatgttg tgccacgcac aatgctatcg
    66481 tctataacca ccaaattgtc tatgcctttt ctgatggtgc cataggtaat atcataaacg
    66541 tgtgtcacca tatcgctgcg gtcatcatca ttggtaataa aagtacgaag cttggcatct
    66601 ttaattgcta ttttctcaaa tcttggttta atggaaagaa tttcattgag cttttcatca
    66661 gaaatttctt ttcctaaaga ttttagtttt ttcttcttta cttcacgcaa atagtcttcc
    66721 atgccctgca tcatgccata aaaagcaact tctgcagtgt taggaataaa ggagaaaaca
    66781 gtgtttctta aatcgttgtc aacagcaggt agaatgcttt tgcatagcaa gcgtcctaac
    66841 tcttttcttt cctgataaat ctctgcatcg ctacctcttg aaaaatatat tctttcaaac
    66901 gaacatgatt tgcgttcaac aggttcttta atgcaaggca ttgaaatgtt cccgtttttt
    66961 ttcattacta atgcatgtcc gggttttatt tcggtaatgt cgctgaacga taaattaaat
    67021 gctgtttgta tggccggacg ttcactggct actactacaa cttcattgtc ggcatacata
    67081 tatgcaggac gaattcctgc aggatcgcgc aacacaaagg catcaccatg acccatcata
    67141 ccgcagataa catagccgcc atcccatttt tttgaagatt gtttaaggat actttccacg
    67201 tcaagatgct cggcaatgag tggtgaaata tcttgtttgg aaaaaccttt ctctttaaat
    67261 gaataatata atctttcatt ttcttcgtca aggtagtgac cgatgttttc cattacggta
    67321 accgtgtcgg ctttttcttt tggatgctga ccaatacgaa tcagattttc aaacaactca
    67381 tcaacattgg tcatattaaa atttccggcc acaacaaggt tgcgtgtcat ccaattgttt
    67441 tgtcgcaaaa aaggatgaca ggcttcaata ccattgccac cataggtgcc ataacgcaaa
    67501 tgtcccatcc acacctcacc actgaaggca agatgtttct tcatccatgc agtatcactc
    67561 agtttggatt cgtcaccatt aacatcagct ttaaacttat tgtagattct tgaaaagata
    67621 tcctgaatgg catgtttgtc gttggaacgg tagcgactga tatatcgtgt tccgggttcg
    67681 gcatcaaatt taatgttggc aacaccggca ccatcctgtc cgcggttgtg ttgtttctcc
    67741 atcagcagat agagtttatt taatgcataa aaaggtgtgc cgtattttct atggtaatag
    67801 tcaaggggct ttaatagcct tatcatggct acgccacatt cgtgatgaag gatatctgac
    67861 atgaagctac aaagatatag caaaaatgag tacacggcaa catgccatta cttaattttt
    67921 gtcttttagg atatagagat gacagtaggt gacaatgtct gctatcagaa acttaatacc
    67981 tttaaagaaa attagcaaac cccgtcttct gtagatgtaa tgttatttat gaagctgaaa
    68041 tataaactcg aggccactgt cttttctggt ttttgcagaa attgttccgc catggagggt
    68101 aattgcattt tttacaattg aaagtccaag tccggaccca ccggtttctc tggttctgcc
    68161 ttcatctact ctgtaaaacc gctcaaaaat tcgaggcagg tgcttttcat ccgaaatccc
    68221 gactccggtg tcggcaaatg aaaaataata ataattttta tcttctttaa gcagcgttaa
    68281 aaagatactt atgtctttgc cggcataccg aatggcattg tcaaataagt ttttaaatat
    68341 tgattttatt aaactttcat ttgcattgat gatgatttgc tcaggcagct tcaagtgtag
    68401 cgtaatgttg ttgtctcgaa gttgcagagc atattcttcc tgtatttttt ttaagagaaa
    68461 agaaatgtca agtgggctct ttttaaaggt atatccggct tcatcaatct tatttatcag
    68521 attcatgtcc tgaaccaact cggttaagac ctttgtttga ttgaatgctt gggcaataaa
    68581 atattgctgt gtttttgcat caagattctt ttctaaaaca gtttctaagt agccactcac
    68641 tccggtaatc ggagttctga gttcgtgcgc tatgttgctt atcatctgtt gctttaaaat
    68701 ttttacattt tcctgcttag taatatcatt gatgacaact tcaaaacttt tatcttcaaa
    68761 tattatagcc cgtacagaga atgtttttcc ttgtttttga attttatact ctttaaagtt
    68821 gtctttatca tcgtttagaa actgttgcag ttcttcaaat gccctgtcaa caaaaataac
    68881 cttcgggtct gaatagtttt catcagtaat aatatgagtg tattgaataa ataatccgtt
    68941 atacagctca gcattttttt taacagtaaa aaaacaaatt ccttcttgag aactatggat
    69001 gtgttgcagc agtttttcac gttctaattc aattttgttt ttggtagctc ttaattgttt
    69061 gtagttttca gctatttcct tacctatttc accaatttca tttttaggaa aatttaatgc
    69121 ggcttttgcc tttgggttaa ctgcataatt acggagttca tttatagaat tgccaaagct
    69181 atttgcaatc caattgacaa gtattaacaa aatacagaac aatagtagtg taacataaag
    69241 aaagacattg tctgacttaa aaaatctttg cacttgaaca tcgtttggaa gagccactcg
    69301 tacaaaatag tctccttcgt tttttgcaaa atacagatat tcttttcctg tggagagaga
    69361 atgtctgatg tctgttccgg ttctgttttt aatggagtta gcaatttccg gtcgccctaa
    69421 atggttttca agtttgttcc atcctaaaat agaattatca taaagcacct taccgttcct
    69481 attgacaatt gttaacctta aattagctgg aaataaggta gagagattat ctaaaatcag
    69541 aatgtactga tcgggagttt tttttgcgat gctttcattt acaatatcgg cataagctcc
    69601 cagtttttct tcaatagctt cggacttaat tctcttttca cgtgtttgct caaacaagaa
    69661 aacacttagt gcaaataatg caaatgcaat ggaaatgtat agaaaaagac gttgcttgaa
    69721 agtaagtttc atagtttcat taagtcgaac ttatatccgt agcctaagcg attggaaata
    69781 atattgccat aatcttccaa tttctttctt aaccgtgtaa tatgtacatc aactgtacgc
    69841 tctgtaacaa acacgttatt gccccaaaca ccttcaataa tttcctgtct tgaaaaaata
    69901 cggtttgggt ttttggcaag tttttgcaaa atttcaaact ctgtttttgt taagttaaca
    69961 ggttttcctt tgataaatgc ttctttgttt tttgtattta tttcaaggtc tttaaatgca
    70021 agtgtttctt tttgttctgt tgaagcagtt ttgaatcttt tcagaattgc tttaattcga
    70081 gcactgactt ctttaatgga gaaaggtttg gaaatgtaat catcagcacc tacagagaag
    70141 ccggtcagca tatcgttttc tgtattctta gcagttaaaa aaatcaccgg gattgtactt
    70201 ccatcaagac gaattttttc caacatttta taacccgaca tgccctccat catcacatcc
    70261 agcaaaatta attgtgtttg gttggtgagt tttgtcagag cttcttcagc agaataggca
    70321 gtatctacaa tgtagccttc gcttttaagg ttgaattcaa gaatttcaca aatacttttg
    70381 tcgtcatcaa caattagaat attctcgttc atttaggagg ggtaactgtg gtcaaagata
    70441 attaatagtt tattttagaa gttgttactt ccggaatgtt ttagtactct ggcgtcaaca
    70501 aaaaacacaa tttcttctac aatgttattg ttatggtcgc ctattcgttc taattttcga
    70561 atcaagagta aaagtttcag catacaaatc gtttgagccg gattgttttt aatatgagta
    70621 gctaatgttt caaatacatt attataaatt tcatccacct gatcatcttt tgaaataatt
    70681 ttttcgctta ctttagtttg ttctgtttca aaggcaacaa aaccatctaa tagcataccc
    70741 aatagagtat caaacatttt ttcaatatta agtcgttcta tcaagtccgg atcaagagtt
    70801 tcacattctt tgtcggttat aaagcgtgca atgccatcag taaaatctcc aatacgttcc
    70861 aaactactgc ttattttgat gagggacaaa gcaagcctca ggtcaatggc aacaggattg
    70921 tttaaggcaa taaaattttc acaaagattg tcaaccttta gctcaaaagt gtttacatca
    70981 ttttcgttcc ggattatttt tgttgccaat gctatatcgt tattcagcat ggcttttttg
    71041 gcattttcaa gctgtgagat aaccagcttc cacattagtt ttacctcatc tttcaacagt
    71101 tgaatttcct tctcagtatg tttcattttt tgaaatatta aatattttat ttgacttttt
    71161 tggattgttg tttatccaaa tcttcctgta atgtagtttt gagtttctgt tttgtcaggg
    71221 gctaaaaaca tttttttagt ttcagaatgt tccaccaatt caccatacat aaaaaaccct
    71281 gttttgtcac ttactctggc tgcctgttgc atgttatgag taacaataat tatagtgtag
    71341 cgttgtttta attcaaaaat aagctcttca attttagcag tagaaatggg atccagtgct
    71401 gatgcaggtt catccatcag aaggatcgag ggcgacacgg ctaatgctct ggctatacaa
    71461 agcctttgtt gttgccctcc agaaagttca aatgccgact tatgaagttt atctttcact
    71521 tcattccata atgcagctgc agttaatgat tcttttactt tttcttctat cagttgtttg
    71581 ttgttaatac cattcactct aagaccataa gcaacatttt caagaattga tttgggaaaa
    71641 ggatttggtt tttgaaaaac catgcctacg ttttttctca gtaaatcaac ctgaacagat
    71701 ttatgataaa tattttcatt attgattaaa cattctccgg ttagttttgc accgtctatt
    71761 tcatcattca ttctgttaaa caaccgaagg aaagtggatt tgccacaacc tgacggtcca
    71821 ataagtgcag taaccgaatt gtcttctatc tgcatgttta tatccttcaa tgcatgaaat
    71881 gtatggtaat agaaatttac atttttagct tcaatcattt tttgtttttt tgaaattgat
    71941 ttttaataac tcctgcaact gtgttcatca gtaaaacaat gatgattaat actaatgcag
    72001 ttccataagc aacaggccgc gatttttcta tatctgttcc gcttgtagct agtacatata
    72061 aatggtatgg taatgccatc acttgatcaa agatgctact tggtaatttg gggagaaagt
    72121 aagcaaccac agtaaacaaa atgggagcag tttctcctga aactcttcca atggaaagca
    72181 ctaagcctgt gataatatta ggaaaagctg ttggcagaat aattctttta atggtttgaa
    72241 ttttattggc accaagcgcc atgcttcctg tacgataggt gttgggtact gctttaaggg
    72301 cttcttctgt ggttcgaatt accacaggta aaatgagtaa actcagtgtc agagacccgg
    72361 caataataga atctccaaaa gctaatgtgt ttacaaataa agtcatgcca aacaatccaa
    72421 aaacaataga aggaatgctg gccaggttat tggtcattat acggataagt ttagttaccc
    72481 aattgttttt tgcatactca acagtataaa ttgcagacat tactccaagt ggaaaagcta
    72541 tgattatgct gcctaaaaca agataaagcg tacccacaat tgccggaaaa attccaccgg
    72601 aagtcattcc ttcttccgga tatgagaaga tgaaatccca actcatcacg cttatcccgt
    72661 tgaagcaaat aaatcctaaa atccaaataa gtacacccaa ggttattata ccaagaatgc
    72721 ggaatatggc aaaggcaatc tgttctttag ttttttttga aataatcatt ctttaataat
    72781 ttttctccct gatatttttt ctgaaattat gctaatgatt aaagtgatta agaacagaac
    72841 acaacctaaa agaaagagtg attgataatg tgtaccacca cccggggctt cacctaactc
    72901 agctgcaatg gttgctggta ttgttctaac agattcaaaa agtgagtggg gcatcacagc
    72961 agcatttcct gttaccatta acactgccat ggtttcgcca atagctctgc ctatacccag
    73021 aataatagca gccgaaattc cggatgatgc ataaggaata acaactttat aaatggtttg
    73081 ccaacggttg gcgcctaagg cataacttgc atcacgcatt gcctttgggg tgtttcttaa
    73141 ggcgtcttct gaaatagtga tgatggtggg cagtgccatt atggctaaaa ttaagctacc
    73201 tgtaaatgct gtttctccta atgttagacc tccaactttt tgtactattg gagccactac
    73261 tactaaacca aaaaatccat acactacaga aggaattcct gctaataatt caatagcagg
    73321 tttcataact tttcgcactt tttcacttgc aatctctgat agataaatcg ctactcctac
    73381 tccaaaagga atggcaataa ctatagctat aaaactcact aaaagtgttc ccacaactag
    73441 tggaagaatt ccaaataatg ctgaaggtgt tgctgtaggc agccaggtag tacccaaaaa
    73501 gaaatcagaa atgcttatta tgtctgtttt caattctttt atgccagaca tatttttggg
    73561 aacatatttt agaggtaaaa acgctattat tgttgaatcg gattgaataa tttctcccag
    73621 tttttgaggt aataatgaat agtcctcccc caattcttca tcagagtaca tagtaaagat
    73681 ttcatcaaat ctgaaaattc gaatatcatc attactacca ccaacatctt tccagttttc
    73741 aatctctgaa tcaaatattt ctttaatctc aacagcggta agcctgttaa caggattttg
    73801 tgtattaact actaatgtat atcctttctc cacagttgaa gatttaaata accctaatcc
    73861 ctctttaaaa agaaaaataa tgataagcag cactgttatc cctgtaacag cactgctcat
    73921 tttaagtaaa ctctcaacaa atttttctaa cactttattc attgtgcagt tttcgaaagt
    73981 aaacttactt gatagtaata tatcctactt tagaaactac attctgacct ttaggggtta
    74041 gaataaaatc aatgaatgat tttccttctg cacttaagtt ggtgtaataa tagtaaagag
    74101 gtctgacaat agggtagctt ttattttttg cattggctat tgtaggttca ataaatgttt
    74161 taccattatt gtacgaaaca ttaagtgctt ttactttgtc tgtaacgtaa gcaaacccaa
    74221 catatccaat tgcacctttt gtttggctta ttgactgtat gatgccaccg gttgcaggca
    74281 tacttaatat gccattcatg tagtttttgt tttttaacac actttcttta aaaaactcgt
    74341 aagtgcctga agaagtttct ctcgaataag gtattatctt catatcgtca cctccaactt
    74401 ctttccagtt tgtaatcttg cccgtaaaaa tcttttctaa ttgttcacgt gtaagttgcg
    74461 atactttgtt tgacggatga acaacaatac tcaatgcgtc ataagcaata atcacctcct
    74521 taaccgcttt accggcttca gcgagctttt gtttctcgtc aaattttatt ttgcgcgatg
    74581 attgtgcaat atctgttgtt ccgtttaata atgctgtaat tcccacacca ctacctccgc
    74641 cagttactga gattgaagca gatggtttct cttttttata tgcctctatt tcttcctggg
    74701 ttagtggcaa aacagtatca gaccctttga ttttttgtgc ataacttccg gatagtgtaa
    74761 ggataatcac tattgccgtt aaaattgatt ttttcatttt tattctatat ttttttgcaa
    74821 agaaaaacct cctatgttgt gatgggctta ctttgctgtt acatttcaat tacgattaaa
    74881 atttatattg gagacggcag gtaaaagcat tgtctttttt gtcggtttca taacctttaa
    74941 gattaatgca tttttcattt tgaactaatt cataatagcc ggttaaacgg aaattggtat
    75001 tgattttcca ctgtaatcca aagcctgtgg tatttaaagc cacatcgcca gcatcggtat
    75061 tgttttttcc aatttcatca ccttttgatt ttgtatttgg gtcgtaccag tcgtacttaa
    75121 caatagcgga aaccggcagt gtaccaaaat cctgaaccaa cataacataa cctccattaa
    75181 attgtcgcat gtaaacatcg ttggtaggta aggttgcagc atttgggctt ttactgtttg
    75241 attttgctcc gggctgtgtt ccaaaaagat attcagaagt aattttagta agtccaatag
    75301 cactatatgc tgtaagatat aaattaaatc caacatattc tctcttggca aatgagccta
    75361 aattgtttgc acttgaatca acaataaacc cattgcctga atttttataa atatactttt
    75421 taccttgata tacaccacca ttataataag aaactcctgc accccatttt attatatctt
    75481 taattgaatt attgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    75541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgt
    75601 atgcaaattt tattcttccc cttctgattc caactctgtt ataagatttc tctttattct
    75661 cgttggcaga acctactttg agtgaggcat ctttttcgcc atactgaaac tgcgtttgga
    75721 tgtagcctga aacagaaaga tttcttacgt ctttcaaagc atcttcaatt tcataaattt
    75781 tttgttctaa gtcggatatt gactttccct gaaccattgt tgtgtcgtgt tgtgcaaaca
    75841 gggttgtgcc cgttaaaatg tacccgacag ttaataataa tttcttcatt tttattctta
    75901 ttaaatttta tgcaaagaaa ttggtgatct atttcaacaa catttcttcc taattacatg
    75961 ttggttaaga tttgctttgt catagatgac aatttgatac ctctttgatt tgctaatgta
    76021 caaatcattt aagcctatga gcccaattca aatgtggttt attataagtt ttcaagtata
    76081 tgtacctgct aacgatttta tttatcaaat taaatgaaaa taacttttac aaaagaggta
    76141 ataaacttta atggtgaagc aaagatttta aagaagaaat aatttgagtg tttttatgtt
    76201 ttatttaaag ggtgttttta ttttatggag tttaaaattt tagagaacaa gactccgttg
    76261 attgagattg aaacaaaatc agaaacaaaa tatcaggtaa tctttaagca cctttcgcgc
    76321 tgttcgataa gtgccatagt tattaaagtg tttttgtcag aattatctga gtaactggga
    76381 gatgaatttt atgtatatta tcttgactta cttaataaca gcatgcttag taacacaatt
    76441 gccaaaagat atgaagtgta gcatgaatca ccgcaaattt taattcttga cagggggaaa
    76501 tttatttatc atgcttagca tgatgtagtt tcactaaaaa aggtaatggc tgcaattgca
    76561 gccattacct tttacgttct cagaaaactt caacgctaat caagtggtaa gccaaaatgt
    76621 gtcatggtgg cttcatagtt tcttaaatca gggtcgtgtc cacttgggtc actctttgca
    76681 ggtataatca aaacttttat gttgcttaaa gaaatcaacc tgcctgtgta aggtgtgttg
    76741 tcaaggttac gtattgtgag cgtaaagcgg tgccaggaaa gtacttgaat tggtgtatca
    76801 gggtcaacag cttgtgttga tgaagttcta taagttagtt tgccttctac gccatagttt
    76861 ggaattactc cgggaacata tatccaacgc tcattaaact ttacatacat agctttgaaa
    76921 ctggcatcga atgttcgttg tgatacatct cttttgttga tgtcaacaaa tgtttcgggt
    76981 ttgtctgcag aaagttttgc cgcgccaaaa agaaaaactt taacatatcc ttggccaggt
    77041 gctggcacca atacagtgct ggaattgatg aggcgttcgt ctgttacact tacatcagtt
    77101 gttgccgctg tggtgttttt gttttgtaga gcctgtgggc tggtgaggtc gtccttgctg
    77161 caaccgataa ttaccgaggt tgctgctaca cttaatacaa taatcagctt tttcatcttg
    77221 actaattttt aactgttaga cattttgttg gttaacgtat attgatagag agttttacct
    77281 ctctttgtta atgcaacttt ttgctattta ataaatcagt gtaaaaatta attcatgctg
    77341 tttgatttga tgatttgtac ttgacttttt aacttccttt gtatttgttg ttgtgaaatt
    77401 gatagtgtaa aggtaagtcc gcaaactcct gaaagcaata cgtaaaactt cgtattttta
    77461 aattattaat cttaatagct gaaattattt cttgtagaaa aattattcca ccagcttatt
    77521 ctctaatgca aagcgtacca acgatgcaat gttgtttaat tttagtttat tgaggatatt
    77581 gtttctgtgt gtatcaaccg ttctggttga gatagaaagc acttcaccaa ttttatcact
    77641 cgttaatcca tcacaaacca atttaataat ttccttttcg cgtgcagaaa tcaggctatt
    77701 ggaataatct aaataggcaa ttgcattttt acggatttga ttaggattat cattctgaaa
    77761 ataatgttta atttgctcac ttaagaaaat ttcattgttg taaatctgtt taaatgcttt
    77821 tataaaatca ttatgatcat tgccttttaa tagataagca tcggcacctg cttttaatgc
    77881 ctgtgcaacc gtaagcatat ctgctttcat acttacaaaa actatttttg tttgcgttct
    77941 gaatctctta atctctgcac aggtttctat tccgtttaac aatggcatat taacatccat
    78001 gataataata ttataatctg tattcaatac ttttccaata gctgattttc cgttataaac
    78061 tgtatcaata ctttctattg acaactcctg ctttaaaaga taggtgaggc cttcggcaaa
    78121 aagtttatgg tcgtcagcaa ttaaaatttt aagattactt agttccatct taaataatta
    78181 tgctttttgc attgaatgtt catagtcaat tttaaattca catcgcatgc caacaccggg
    78241 ttgtgtaata aactctgtct tggcgcctaa ttcttctaca cgctgtaaga tattattcag
    78301 ccccttgcca cgaccgttcc cttttatatc aaagcccaag ccattgtctt catagataaa
    78361 ataaaattta tccctatctc ctaaaacaat gatgctgata atagaagcat gagcatattt
    78421 taaagtgtta ttgatgagct cctgagtaat gcggaacaga tgatattcaa catcaagatg
    78481 taatcttttt tcaacacctt tggtatagaa gttacaatga ataggctgaa attccataat
    78541 ggtgttaagt agtttttctg ttgccttctg aaaaccttca ttttgcaaag ttgaattttc
    78601 taattcgtta gtaaccgacc gcagtttgtt aattgactct tcaagtagtt gcatggattg
    78661 atctaccacc ctttcatttc catcaacacg agccttactc attaacattc tggcaagtga
    78721 tagttgtgcg ccaatatcat cgtgcagttc gcgcgatagg ttatttctaa gttttaaaat
    78781 atctaactgt aattgttgtt tttcactttt ttcagtaaga aattttttct ctacaaatag
    78841 tttttcattt tctaccaact gagttttata agccaatccc aaagtaaaag ttgtgagttc
    78901 gccaataaca cataccatca atggcagatg aatgtcaaaa ggaatatcaa atcctctaaa
    78961 ctcaagaaag ttaagaacaa tgtcaaatat tgatcctact gcaattaatg atgcccccgt
    79021 catggcaaat ctgtcgagtg gaatactctt ctttaaaaaa ttaattatta taaataatcc
    79081 ggctgtgatt gtaattattg caacaacgtt aaaaatcctt tgccagacaa caatatcaag
    79141 agaaagccag ataaagaaca gactgagtag cgtataagca aacagaaaaa tttcgagttt
    79201 tttaaccatt gcattaatat cgggtcgtct atgctttaag ttcagaaact cacgcataaa
    79261 cctgaaataa agaagaatac aaaacagcgg tactgatagt tttatatacc cataatactt
    79321 ctccgcaatt tcagaaaaag gataaaaatt taaatacttg tcatattggc ttagtccaaa
    79381 aacgataaca aaagacaagt atgcaatata aaacaaataa tcccttctgt gctcgataag
    79441 ccattgcaca aatacataaa caatctggaa cgcaactata gaaacaaaga tggttaatcc
    79501 atataattta ctttcatgtg tgtagaacaa aaaatccatg cttctgaagt ttttgagcct
    79561 gtttaattat tgtggtttta ttctgactaa acaatcattt tcagttaaaa aacacttgac
    79621 tcaacaatgt taacattaat caggtatttt accatagctt tttgtaccaa tggtattttg
    79681 agtatgttaa tggaattttt ttgtcttaat tcgggcagat tttgttaata cttagatggt
    79741 tctatcccct gacaatcaat agcccttaag aataattaac aaaaataaga tgttggaata
    79801 gcgatttttt atacttttgc ccattcgttc ccaaaatagg aagtacgtag tggaaaagat
    79861 gtcgaaatac aacctgcatt atgccggttt aaaagagggg atacatgagt ttgattactt
    79921 gctgaatcag gactttttcg acatgtttga acaccctatt gttcagggag cacatgttct
    79981 tattcatctt accgtaaaca agagtgccac ccttgtggtt ctgaatatta aagctgccgg
    80041 tacagtacat gtacagtgcc atcgttgcct cgaagagttc gagatgtcat tgaatataac
    80101 caagactatt atcgttaaaa cttcaggcga agacaaggaa gtggatgatg acaacatcat
    80161 tttagccgac aatgcccacg atattcatct tgcacagcat ttttacgatt tagtttcatt
    80221 agctataccc ataaaggtaa ctcatcctga caatggctgc aatgaagaaa cccttcgtac
    80281 acttgaaaag tatatggtta agaatgaaaa cacgaacgac ccacgatggg atattttaaa
    80341 gaatgtaagt aacaattgat attaaataat taaacgtcat gccaaatcca aaacacaaac
    80401 actccaagtc gagaagagat aagcgcagaa cacactacaa agcagaagct ccaacaattg
    80461 ccgtttgccc taccacaggt gaaacacatt tatatcatcg tgcctactgg catgagggca
    80521 aaatgtacta caaaggcaag gtggttttag aaaaagctgt ttaatccctt tattcctata
    80581 tgataaggat aggagttgat attatgggtg gagattatgc tccgctcgaa actaccttgg
    80641 gtgcaattgc tgcattaaac gacctttcac ctcaggaagt acacctggtt ttaataggtg
    80701 acgagaaaaa aataacggag ataattgacg gacagggagt caacccaagg ttatttacaa
    80761 ttgtaaatgc tacagaggtg attgagatgt ctgatagtgc aacacgtgct ttttctcaaa
    80821 aacccaactc aggtatggcc attggttttg gtctgctaaa gcaaaaaaag attgatgcct
    80881 ttgccagtgc cggaaattcc ggtgctatgt tggttggcag catgttcagc gttaaagcta
    80941 ttaacggtat tcagcgtccc gcaatctcaa ctatcttacc acaggaagac ggttcaacag
    81001 gaattttact cgatgttggc gccaatgccg actgcaagcc cgaagtatta caacaattcg
    81061 gattgctcgg gtcactgctt gcagaacacg tttatcatat tgcagcacct cgtgtggcat
    81121 tgatgaatat tggtgaagag gaagaaaaag gaaatctgct ttcgcagcag gcacatcagt
    81181 tgcttaaaaa cactaaggaa ataaatttta tcggtaatgt tgaagggcgc gacttgttca
    81241 acaacaaagc tgatgtcatt atatgcgatg gttttacggg taatgtaatg cttaaagaag
    81301 ccgaatcact ttatacacta ctaaaaaaac gtggtattaa agatgaatac ttcgaccgct
    81361 ttgattatga aaattatggt ggcactccgg tgcttggtgt aaatggaaat gtcattattg
    81421 cacatggtat ttcaaaatca aaagccataa aaaacatgat attgcttgcc aaagaagtta
    81481 ccgaagcaca ccttgcagaa aaaatcgcaa ctttttttca aaataacgta gttaatcaaa
    81541 cactataatt tttttgatgg aaaaaatcag agctgccatc accgctgtta atgcctgggt
    81601 accggactat gttctcacaa acaaagaact ggaaactatg gttgaaacca acgatgagtg
    81661 gattcaaaca agaacaggaa ttaaagaacg cagaatatta aaagatgccg gtgaaggtac
    81721 atccaaaatt ggtgttgaat cggtaaacgg actattaaag aagcgtggca tacaggcaga
    81781 agaaattgac ttactgattt gtgccactac tactcctgat ttggttttcc ctgccactgc
    81841 aaatattatt tgtgataaag taggagctaa aaatgctttt ggctatgata ttcaggcagc
    81901 ctgttcagga tttttatttg cattgaccac agctgccaag tttgttgaaa gtggacaata
    81961 taagaaagtt gttgtggtag gtgccgataa aatgtcggca atcattgatt atagcgatcg
    82021 tgccacatgt atcatttttg gtgatggtgg tggtgctgtt ttattagaac cggataccaa
    82081 cggttacgga atttacgatg ctgttttaaa aagcgatggc agcggacgac attttctgca
    82141 tcagaaggct ggtggcagtg ttaaaccgcc aacgcatgca acaattgatg caaaagaaca
    82201 ttttgtctat caggaagggc aaaccgtatt taagtttgct gttaaaggta tggctgatgt
    82261 aagtgcagaa attatggaac gcaacaactt gagtgctgat gatattgcat ggcttgttcc
    82321 acatcaggca aacaaaagaa ttattgatgc tactgccaat cgcatgggca ttggcatgga
    82381 aaaagtgatg ctcaacattc agaaatacgg taatacaacc aatggtactt taccactctg
    82441 tttatgggaa tgggaaaaac aattgcataa gggcgataac ctgattcttg cagcttttgg
    82501 tggtggtttc acatgggggg ctgtttatct taaatgggct tataacagtt aaggaacaat
    82561 aagcttaatc aaacatttaa ttttatcgtt ttgcgtgttg gctggtgcga cttggcaaac
    82621 cgcttgggtt cacgaacacc attttataaa aaaaatagtg agagctccgt aggagcgata
    82681 tcataatagc tccgtaggag cggtattact caatccattc aaacaaatat ttttcatcat
    82741 aacctacgtc aaatttttgt aacagttcta aatactcgtc tctaaatgtt ttctttttgt
    82801 gatgttgttc ttgattgttg atgtatgcaa taacggtatc taattgagac tgggcataag
    82861 aaaatgcacc aaagccttct tgccattgaa atttttcttt tactaatcct ttttcattta
    82921 tccattttga tgaactggct ttaatgtctc taaccaaatc tgataatgca atattgggtt
    82981 ttatacttac aaggatatgt atgtgatctg caacgcctcc tatagcataa actttttgtt
    83041 cttttccatt tacaatgcca caaatgtatt tatgcaattc ttctttccag tctttttgaa
    83101 tcaggtttag tcgtcctttt actgaaaata caatgtgtaa atatatttgg gtgtatgtgt
    83161 ttgccattat ttttatttcg ccccgatggg gctattagga tttcgctcct acggagcttc
    83221 ttgtttcggg ttgtttcgtt tttttattag gatttcgccc cgatggggct tggctattgt
    83281 gcttgttggt ttattaatat attgctccta cggagctgtt tttttatcta tatttttcat
    83341 ttcttctttc gaaagctccg taggagcgat attgtaatag aatgcaatca taataaaatg
    83401 taatcatagt agcaataatt atagctccgt aggagcgata tcttaatagc tccgtaggag
    83461 cgatatctta atagctccgt aggagcg
//
LOCUS       DAOQNP010000002        77493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_82_length_77493_cov_9.156138, whole genome shotgun sequence.
ACCESSION   DAOQNP010000002 DAOQNP010000000
VERSION     DAOQNP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 77493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77493
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82_length_77493_cov_9.156138"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKY09_00430"
     CDS             <1..422
                     /locus_tag="PKY09_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HNB11343.1"
                     /translation="GFETAVRIIPYMVGMLVAVSMLRTSGAFDLIIDPVKHLIAISGM
                     DTRWAEGLPTALLRPFSAGGARGFMIDAMRTFGPDSFIGKLVSVLQGSSETTFYVVAL
                     YFGSVSVKDTRYAIPVMLLADLAGVIFSIAICYLLWA"
     gene            562..2754
                     /locus_tag="PKY09_00435"
                     /pseudo
     CDS             562..2754
                     /locus_tag="PKY09_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
     gene            2782..3297
                     /locus_tag="PKY09_00440"
     CDS             2782..3297
                     /locus_tag="PKY09_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB11344.1"
                     /translation="MAGTISLDVSHSWMQFGISHFLTTANGTLAVDTGYINITNDLAT
                     STIFVQLNVKSIDTKNTKRNNHLLSSDFFDAEKFPVIVFKSNKIEKAAEGSAFRYIAK
                     GELTIKDITKPVELRFNYMGALPNEYAAKDAHTFVGEADLDRIDFSVGESGPTVGNKV
                     KATFSVEGYKI"
     gene            complement(3377..4090)
                     /locus_tag="PKY09_00445"
     CDS             complement(3377..4090)
                     /locus_tag="PKY09_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB11345.1"
                     /translation="MIVDLLIPCYMDQVFPETANNVVKIIEKLGCGVNYNVEQTCCGR
                     TAFEEGYQKECKAVGEKLIKEFQNERYIVCPGAYCSGMIKNIYPQMFHNSSLHNEYKS
                     VQKNIYEFTDFLVSVLNVTDVGARFNGKVVILDSCKAINELSVKDSVRTLLSKVKGLE
                     WIESTDSCCGYSPSFTDKYEAISVDIGKRKIQELIQTGASTVISTDYTCLMHLKSIAD
                     NEVSSLQFMHIADVLASGW"
     gene            complement(4119..5408)
                     /locus_tag="PKY09_00450"
     CDS             complement(4119..5408)
                     /locus_tag="PKY09_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB11346.1"
                     /translation="MKKNDPKVLRAWTFYDWANSVYSLSITTAIFPIYYNSITSANGS
                     DEVTFLGRTFTNTALYSYALSFSFLVIAFLSPLLSGIADYKGNKKFFMRLFCFLGSAS
                     CASLYFFKDLSSLDMGIISFILASIGWAGSIVFYNAFLPEIATADMQDKVSARGFALG
                     YIGSSLLLIFNLAMILNPSWFGITNSGMASRISFLMTGAWWFGFALFTFKYLPEKTST
                     HNTSQQLLTKGFNELLKVLKQLQSLPMLKRFLISFFFYNMGVQTVMYVASLFGTKELH
                     MRDDELIITVLIIQFVAIAGAYLFASLSSKAGNIKALLIGVTIWIGICWAAYFVTTSV
                     QFFVLAFAVGMIMGGIQSLSRSTYSKMLPKTQDNASYFSFYDVCDKIGLVLGTVAYGY
                     IEELTGSMRNSILVLMTFFVVGLFSLLSIRNLKLQST"
     gene            complement(5425..6201)
                     /locus_tag="PKY09_00455"
     CDS             complement(5425..6201)
                     /locus_tag="PKY09_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11347.1"
                     /translation="MEFRLWHCRSTGTKKPNWLLVLSQILSPLLVVSFLNFGCKKKSD
                     DSPSPFLGITVYNDFKTHPDTFIFKAESTIPCNYKWNFADGSTEVTGDSVMHLFDYGF
                     FNIVLKGETAAGKTATTIKGINASPYTKANIINFSLNSWPVHKSDGTAWDSDGSAADI
                     YCQIWNGNTYVSTTVSNNAPQGTAINFNFITPFAITQFDKSIIIKVFNYNLSPETDDL
                     METVVIEKNLTPLMTNQLPYAVQQSFSGSQTNGTLSFIWQ"
     gene            complement(6201..6602)
                     /locus_tag="PKY09_00460"
     CDS             complement(6201..6602)
                     /locus_tag="PKY09_00460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB11348.1"
                     /translation="MPEIITPIQIRFSDIDWMGHVNNAVYLSYIEKARIDFSERLPLN
                     INWRETGFILAQTQINYRHPALLNDNISVRTWCTRVGTKSFDLGFEVFKTTDNNKIIL
                     ADGLTVLVCYNYRLSVSENLPKEWKKLLLKK"
     gene            complement(6605..7855)
                     /locus_tag="PKY09_00465"
     CDS             complement(6605..7855)
                     /locus_tag="PKY09_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11349.1"
                     /translation="MNRILLFVFAFICSGQSFAQCLDPYYPRIYQTYVERDISYGSDT
                     AYNNTVANLQMNIWKPIGDNNTSRPIILWIHGGGFFTGNKNDMDVIAQAYAERGYVSA
                     TMSYRLGFYGNLFFGAPFTFDSSEVIRAAYRAVQDAHGAMRFLKARAAIDSSNINLAF
                     VGGASAGSITAMHFTFVDKQSEAPINGGAISDVTTLFGNVQRSDLGSIFGRLNLNGQS
                     SNVLAAVNLFGALLDTSFIEANTDPALFSYHQTGDGTVGCGYLPGLWNQPMNVSQNYP
                     HLFGSCVINDRMNNLGYSSQHYQALLYNGNVHGIHDDVLIDTLVARFLSDILCENISG
                     TNTVDLPAVHVYPNPAKGQININIPGKTFSCSMFDVQGRNIIPSTPLLHDYTTLNTTG
                     VSKGMYLLNIITDKQLISKRIIIE"
     gene            complement(8082..8621)
                     /locus_tag="PKY09_00470"
     CDS             complement(8082..8621)
                     /locus_tag="PKY09_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB11350.1"
                     /translation="MTTLTKTTWGIDQAHSEITFKVKHLMISNVKGNFAKFEGTVQSD
                     DNNFSNANINFSMDASSITTGDTQRDTHLKSGDFFDAENHPSISFKSESFSKTGDDIY
                     ALNGQLTMKGVSKPVKLNVEFGGMMKDPWGQEKAGFTLSGKINRKDWGLNWNTALETG
                     GVLVSEEVQIAAEIQLVKK"
     gene            complement(8656..9378)
                     /locus_tag="PKY09_00475"
     CDS             complement(8656..9378)
                     /locus_tag="PKY09_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB11351.1"
                     /translation="MENKVIHRAENRGNADHGWLKAKHSFSFANYYNPNQMHFGVLRV
                     LNDDHVEPGMGFGTHPHDNMEIITIPLAGALEHKDNMNNHGIIKYGDVQVMSAGSGVT
                     HSEFNASKTEAVKLFQIWLYPDKENVNPRYDQLSLNISDRKNKLQTIVSPDTEKSGLW
                     IHQQAWLSMGNFEKDKNTSYKIKKEGNGVYAMVVKGEFEINNNVLYERDAIGLWNTSE
                     INFIAKTEGAEILLLDIPMQLN"
     gene            complement(9492..10094)
                     /locus_tag="PKY09_00480"
     CDS             complement(9492..10094)
                     /locus_tag="PKY09_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB11352.1"
                     /translation="MAKTDSNKPFNLILKNISRYILLSEEEETIFTSLLSTRLVRRKQ
                     FIMQAGEVFSASCFVNSGCLRAYNIDKNGTEHILQFAPPGWWIGDFYSSITGRPGILF
                     IDAIDESEIVVLTKENRDILFDKIPKFERFFRILIENSMASQQERILDKLSLTAEERF
                     EKFCLKYPALVSCLPQKYIASYIGVTPEFFSKMRARMTKS"
     gene            complement(10078..10656)
                     /gene="lpcA"
                     /locus_tag="PKY09_00485"
     CDS             complement(10078..10656)
                     /gene="lpcA"
                     /locus_tag="PKY09_00485"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019934331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNB11353.1"
                     /translation="MSEIIRQHFEEAKKVLETFLSDEKNIAAVTKAGELMTASLAAGG
                     KIISCGNGGSMCDAMHFAEELTGRYRNDRKPIAAISISDPSYMSCVGNDYGFNYVFSR
                     FIEGVGNKGDVLLAISTSGNSANVLEAAKVAQSKNITVIALTGKDGGALKNHCDVEIR
                     VPHSGYADRTQEIHIKIIHALIDYIEQHGKNR"
     gene            10829..12409
                     /gene="lnt"
                     /locus_tag="PKY09_00490"
     CDS             10829..12409
                     /gene="lnt"
                     /locus_tag="PKY09_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNB11354.1"
                     /translation="MQVKNKFILALLSAILLSLSWPERGLTPLVFIAWVPLLLLEAYS
                     SQNKEQKKFRLFGWAYFAILVWNALTTWWVCNASFGGGLFAIFCNALFMTGVFYAFHL
                     VKKKKGHVVGYAALIIFWVAFEYLHLNWELSWPWLTLGNVFATKPQWIQWYELTGVLG
                     GTVWVLLVNILITRIFLASADKLKRNIMYATIAIIFPVLISILRYYTYKEEENPVNVI
                     ATQPNIDPYGEKFSGMSNVDQLIQVLRLANLELYDSSDVILAPETAIANSIWEDEIEL
                     YPEIKLIRNYLQANPGATIIIGASTNLAYLNGQKPSATARKFKDQDGYYDSYNTALLF
                     ENGKPAQIFHKSKLVPGVEKMPYPKIFGFLEKLSIDMGGTAGSLGIQDEPTVFTTKNR
                     MIVAPVICYESIYGGYLAKYIRKGAQWIAIITNDGWWGDTPGYRQHFNYARLQAVSLR
                     RSIGRSANTGISGFINQRGDAFQQTPYWKDAVVRQAINKNDKLTFFAQHGDYPGLIAS
                     ILVLPVIVITFFGKRKNQ"
     gene            12448..13851
                     /gene="lpdA"
                     /locus_tag="PKY09_00495"
     CDS             12448..13851
                     /gene="lpdA"
                     /locus_tag="PKY09_00495"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB11355.1"
                     /translation="MENFDVAVIGSGPGGYVAAIRCAQLGFKTVIIEKYNTLGGTCLN
                     VGCIPSKALLDSSEHYFNAVNKFGEHGIKVKDIKADMEQMIKRKAQVVKQTCDGISFL
                     MKKNKITVMTGHATFVDAKQLDVVDASGKSNRIGATNFIIATGSKPSSLPGIEIDKKH
                     IITSTEALELKTIPNHLVVIGGGVIGLELGSVYARLGAKVTVIEYMNAIIPTMDRALG
                     KELQKVLSKEGFTFLLGHKVQSVKSSAKQVEVVAESSNGEKVNITGDYCLVSVGRKPY
                     TENLGLDKAGVAIDERGRIITDEKLQTNVPGIYAIGDVIKGAMLAHKAEEEGVFVAEQ
                     LAGQQPHINYRLIPGVVYTWPEVASVGYTEEELKESGTKYKTGNFPFKASGRARASMD
                     TDGFVKVLADSDTDEILGVHIIGPRAADMIAEAVVAMEYRASAEDIARICHAHPTFTE
                     SFKEACLAATANRAIHI"
     gene            13881..14447
                     /locus_tag="PKY09_00500"
     CDS             13881..14447
                     /locus_tag="PKY09_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNB11356.1"
                     /translation="MNIQIKEIVTVSMILFSVIDIIGSVPVIIGIRKSVGSIHPEKTS
                     LVALMIMIVFLFVGENILSLIGLDVSSFAIAGSFILFFLALEMILGVKLYKDEMPASA
                     SIVPIAFPLIAGSGTMTTLLSLRSQYDMLTIIIGVLLNMIWVYLCLKNLGFIERLLGP
                     TGIAIVRKVFGFILLAIAVKLFRSNLGI"
     gene            14452..15078
                     /locus_tag="PKY09_00505"
     CDS             14452..15078
                     /locus_tag="PKY09_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNB11357.1"
                     /translation="MAKKKFYVVWKGVMPGVYHSWDDCKRQIDGYAGALYKSFESENA
                     ALTAFQKGLSGFQKKGEAHPLIHVEKTASKPIKESIAVDGAWNTKTLESEYRGVEVKT
                     GKQLFLKGPYADGTNNIMEFLALVHALAYCKRHQINLPIYSDSMTALKWVRTKKANTK
                     LASTGRNDELFELIVRAEEWLKNNSYANKLLKWETQIWGENPADFGRK"
     gene            15282..15467
                     /gene="ccoS"
                     /locus_tag="PKY09_00510"
     CDS             15282..15467
                     /gene="ccoS"
                     /locus_tag="PKY09_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNB11358.1"
                     /translation="MSVLFILIGASLVVATGFLVSFFWAVRSGQYDDDYSPSVRILFD
                     NDKTLTSDKSNSNITDN"
     gene            15472..17598
                     /gene="ccoN"
                     /locus_tag="PKY09_00515"
     CDS             15472..17598
                     /gene="ccoN"
                     /locus_tag="PKY09_00515"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNB11359.1"
                     /translation="MTIEKFRYDNKIVAMFAWATIIWGIVGMLVGLLAAFQLPFPVLN
                     FDAHTTFGRIRPLHTNAIIFAFVGNGMFMGIYYSLQRLLKARMYSDLLSYIHFWGWQL
                     IIVGAAITLPLGITASKEYAELEWPLDIAIAIVWVIFGINMFGTMMRRRERHLYVAIW
                     FYVATVVTVAVLHIVNSLAIPVTLFKSYSLYAGVQDALVQWWYGHNAVAFFLTTPYLG
                     LMYYFLPKAANRPVYSYRLSIIHFWSLIFLYIWAGPHHLLYTALPDWAQSLGVVFSIM
                     LLAPSWGGMINGLLTLRGAWDRVRDNPELKFYVVAVTAYGMATFEGPMLSLKSVNAIS
                     HFTDWTIAHVHIGALGWNGFLTFGILYWLIPKLFNTNLYSKKLAGIHFWLGTLGIVFY
                     AIPLYWAGITQHFMWKEFTEDGVLRYPNFLETVVQVIPMYKLRALGGSLYLLGSLVMV
                     YNLIKTVRAGKMVANEEAEAPALEKNYVAHDDHWHRWIERRPVQMLIGSLIVILIGGM
                     VEIIPTFMVKSNVPTIASVKPYTPLELQGRDIYVREGCYTCHSQMVRPFRYETERYGE
                     YSKPGEFVYDHPFQWGSKRTGPDLHREGGKYPDSWHYNHMLDPQTMSPGSIMPSYAFM
                     IDDDLDTSTTAAKIRAMQKLGVPYPDGYDAHANKDLMKQANSIASNLEKDGIKVMGNK
                     EIVAIIAYLQRLGVDIKAEKTTASVN"
     gene            17613..17780
                     /locus_tag="PKY09_00520"
     CDS             17613..17780
                     /locus_tag="PKY09_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HNB11360.1"
                     /translation="MYKEVMSTIKDISIYPVFSFVIFFVFFTIVGIWVLKSKKEDFES
                     VSKLPLEEKND"
     gene            17792..18964
                     /locus_tag="PKY09_00525"
     CDS             17792..18964
                     /locus_tag="PKY09_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNB11361.1"
                     /translation="MKRIINLICLIMLISLSAWAGDNSQATETAKVAAAQPPDWAMNA
                     NTYLIAFIVIVLLVTILALFRSSLKLIKKITSFCIVMFISGSAFAAETTESKEVAKVT
                     AAQPPDWALHPNTYLIAFIVLILLATIIVLYKTNMKLIKMIAPHAFDDEKQTAVEGAV
                     SGVKNRETFMRKVYLRLVDSVPVAKEQDILLDHDYDGIQELDNNLPPWWKWGFYITII
                     AAVVYMLAYHVTGTGKLQAEEYADELAVAAKQKEERMKNSAENITEENVTVLVDAAEI
                     AKGKDIYEKNCSACHMADGGGMVGPNLTDNYWLHGGGVKNIFKTITYGVPAKGMISWQ
                     AQLSPKQIQEVTSFIMTLHGTKPAAPKEPQGDEWKEDATATAGDTTAVASAEAAVK"
     gene            18982..20385
                     /gene="ccoG"
                     /locus_tag="PKY09_00530"
     CDS             18982..20385
                     /gene="ccoG"
                     /locus_tag="PKY09_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNB11362.1"
                     /translation="MVENKMEHIDESFRDSIATVTEDGKRAWIYPKKPKGRYYNARTW
                     VSVVFLIIFFALPLIKINGHPFFLLNVVELKFVLFGIAFFPQDFYLFVLAMLTFVVFI
                     ALFTALFGRLWCGWTCPQTVFMEMVFRKIEYLIEGDFMKQKALDKRPWDAEKIRIKTT
                     KHLVFFFIAFLISNTFLMYIIGFDEWKKIITDNPLNHIGGLFATIIFAGVFYGVYARF
                     REQVCIVVCPYGRLQGVMLDPNTVVIAYDYKRGEPRGKLSKDNNEQGDCIDCNQCVQV
                     CPTGIDIRNGTQLECVNCTACMDACDDIMDKIQKPRGLIRYTSENAIKKNAKFKINSR
                     IIGYCIVLTILFSTFVVLLARRSNIETTILRASGLLYQKPDESHISNLYNIALINKTF
                     GDMPIELKVVEPKGEIKWVGNGITHLKEQSVAQSTFFLILPKTEIKKTKTKVIFEIWS
                     EGEKIDRYETSFLGPNN"
     gene            20393..20830
                     /locus_tag="PKY09_00535"
     CDS             20393..20830
                     /locus_tag="PKY09_00535"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNB11363.1"
                     /translation="MKFNWGHCIAVVLVIFASSILVFVYVASKQKNELVTEKYYEKAV
                     AYQQRIDAEKNALNENAEVKYNIKSGQLMLVLGRNMPNANGTFQFYKPDNAAKDFNTS
                     FQVNDTGFYSAVIPQLTHGRWSINVSWNVDSKAYFYEKKIFIP"
     gene            20830..21558
                     /locus_tag="PKY09_00540"
     CDS             20830..21558
                     /locus_tag="PKY09_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB11364.1"
                     /translation="MNENTFYYGALLLGFLGSFHCVGMCGPIALMLPANKLNNRSVAF
                     FGRMFYNIGRMITYMFIGMLFGLLGAAFVLNGFQKQLSIITGVILLLIAIFTLIPSTR
                     KKVLSVSSKINIPFRGVLKKLFSGKTALHHFGIGIINGFLPCGFVYVATAGAINAGNI
                     LNSVLYMAFFGLGTFPIMLLVSYAASWCNFSMSRYVTKATPWISAALALFLIYRGAFM
                     NFDADCHKHSHPPTMIECSKPETL"
     gene            21649..24672
                     /locus_tag="PKY09_00545"
     CDS             21649..24672
                     /locus_tag="PKY09_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB11365.1"
                     /translation="MSHKLLDALMHLFAILANAEKFSLEGRSIVESFLRQQISRAYVL
                     KYLELFDAYLENIRGKATEGKLKKRLSVNSVKVLRICTDINAELDTRQKHIVLMRLIE
                     FVYTSEKIIDSQEREFLYTVAEVFHISPDDVQLCMELACNNTEDKYSDTPKYLVLSSQ
                     DDENNTLNTKTQSISGLSGFFRFLFLKNAGLLFVRYHGTESFTLNGLPLNDDVCYVFS
                     AGSVIRGSKVNTIYYSDIIRLFSNFKQQSLLHFDVQHVYYDFANGKRGLNDVTFSATS
                     GNLVGIMGGSGAGKSTLLNILNGNNKPTQGKVTINGIDLYRESHLIEGMIGYVPQDDL
                     LIEDLSVYQNLFYNAKLCFGNLTDGEIDERVKALLHNVGLGEASHLRVGNALSTSISG
                     GQRKRLNIALELIRAPEVLFVDEPTSGLSSLDSENVMDMLKQLANHGKLVFVVIHQPS
                     SDIFKMFDKLFVLDIGGFPAYFGNPVDSLLYFKQRASFADADYSECGTCGNINPEQVF
                     SILESKVMDEFGNPTQQRKLTPSDWYQLFKEHSSGYGKVNERQPVQGSANQKPGWIQQ
                     LKVYITRDVLAKLKNKQYMLITFLEAPVLAVILAWFLRYFHAGGSYIFSENKNLVAYL
                     FMAVIVAIFMGLTVSAEEIIRDRKMLKREQFLNLSRSSYLSAKVLVQFLISAIQTFSF
                     VIIGNFIFGIQSMSLDYWLMLFSVSCFANLLGLNVSSMFDSAVTIYILIPFLIIPQII
                     LSGVMVKFEDLNPYLTSQRHVPVIGEMMVSRWAFEALAVNQFSNNPYQKNFFNVDRQM
                     SEATYKKDFWLQKLNDIADDISKNKSKNPKQDVALIANEFHKEALINKNFKPSVDFEK
                     SDALEIASQIKPLTDTLRNFYIEMYNNASAAKDRLVKKALSHSTVARMNKQKEKFTNN
                     ALNDLVLNRNDFTILVQDGNSIVQRFQPVYMQPNPSALFRAPLYVYEKNFLGMKIHTW
                     YVNLFMIWLMSFILMVTLYYNSFRKFIGLMGQGRK"
     gene            complement(24779..27337)
                     /locus_tag="PKY09_00550"
     CDS             complement(24779..27337)
                     /locus_tag="PKY09_00550"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNB11366.1"
                     /translation="MLIETITTESQMTTYEYESVLKASLEYFEGDELAATTWMNKYAM
                     KNKEGKFVERTPHDMHLRMAREFARVEAKYNLLNDGSRTTHLSDYGKKRQQLTTEKIT
                     QLFDRFRYVIPQGSVMSALGNPFMIASLSNCIVLPELHDSYGGIFFADQQLAQLYKRR
                     CGAGLDLSTIRPEGMRVSNAAGTTTGAVSFMERFSNTTREVAQLGRRGALMLTLDIAH
                     PDVEKFITIKQDLSKVTGANISVKLSDEFMVAVNENTDFNLRFPVDSTDPYIQKKVNA
                     RELWKTIIACAHRTAEPGIIFWDRQHHYSTSSVYPGFKNVSTNPCSEIAMQGGDSCRL
                     IAINLLSFVDHPFTQKAKFNFTRFYEVTYESQRLMDGLVDLELEAIERILQKIESDPE
                     PDYIKEVEMRTWITLYEAGKKGRRTGLGFTALADALAAMNIAFDSDNALSIVEMMMKE
                     KCRAEFDSSIDMAIERGTFEGFDTAIEDQSEFVNMMKAELPDIYNRMMKYGRRNISIS
                     TVAPTGTLSLLSQTSSGIEPVFMLSYKRRRKVNAEDSKAKTSFVDQSGDAWQEFEVYH
                     HGLKKWMTANNSTDYSKSPYAGSTANEIDWKKRIKMQAVVQKYTTHSISSTINLPENV
                     SAETVGEIYMQAWSAGLKGITVYREGSRSGVLVKKEEKKAKPIITESSAPKRPEILAA
                     KVIRFMNADEKWIAYIGILDNKPYEIFTGKAEDAFSIPAWVSSGWIIKSKNGHGKSRY
                     DFQFNDKEGFKVTIEGLSRSFDKEFWNYAKLISGVLRHGMPIPQVVNLVENLHLYSDS
                     INTWKNGVMRALKQFIPDGTKVNDKVCAECGDKEGLYFEEGCIKCKSCGHSKCG"
     gene            27616..28992
                     /locus_tag="PKY09_00555"
     CDS             27616..28992
                     /locus_tag="PKY09_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNB11367.1"
                     /translation="MKFKTIIEPFRIKMVEPIHLSTEEQRIGYLKQAHYNPFLLNSNQ
                     VLIDFLTDSGTSAMSSDQWSAMMNGDESYAGASSWLRFEKEVQSLTGMPFVLPTHQGR
                     AAERILYGKLGGKGKTFISNTHFDTTRANIEFSGAEAIDIPVKEGLDFASDFPFKGNM
                     DIALLEKTIAEKGAANIGAVILTVTNNSGGGQPVSMENAKSVANVCRKNKVLFVIDCC
                     RVAENSYYIHHNEAGYGNKTYRQIAEEMMCLGDAAVMSAKKDALVNMGGFLAVKDADL
                     AESCKQLLIITEGFATYGGLAGRDMEAIAVGLQEVFDPGYLNYRIVSTRYLGENIKAK
                     GVNIIYPVGGHAVYVDAKSLYPHIPPHEYPGQALVCELYRIGGLRSVEIGSVMFGKYD
                     SDGKLIPAPMELVRLAVPRRVYTQSHIDYVIEIFDEIVRTKDKVKGLKIVKEPQFLRH
                     FTAHFEPL"
     gene            complement(29070..29894)
                     /locus_tag="PKY09_00560"
     CDS             complement(29070..29894)
                     /locus_tag="PKY09_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNB11368.1"
                     /translation="MKWFLVIKKQARPILLWMLLLLQVAMVGNAQQIPPQSNPPRLVN
                     DYAGVLNDVDENLLEQKLVQFNDSTSTQIAIVLIRSTGEYPVDDYAITLFRDWGIGQK
                     NKDNGVLILAAMEDRRMTIITGYGVEGALPDAICKRIIELKLKPAFKSQQYYQGLDAA
                     VTEIMQRTKGEFVSDGENANKNSPAWAPILFVFLIFGLIMLMKVFSVRRYAHLNNLSF
                     WTAWMLLNAANNRRNSSGGGGVWGGGGSSWGGGSSGGGFGGFGGGSTGGGGATGGW"
     gene            complement(29872..30312)
                     /locus_tag="PKY09_00565"
     CDS             complement(29872..30312)
                     /locus_tag="PKY09_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNB11369.1"
                     /translation="MANAADYFSDEQQLLLKNAIARAEKHTSGEIRIFIDSKVKSEII
                     DRAAEVFENLKMHETKLHNGVLIYVAIENRQFAIIGDSGIHQYVSQVFWDKVKEEMIP
                     FFKRQDYTGGLLHAIEETGQKLKMYFPVAKNDSDELSNEVVFGD"
     gene            complement(30315..30905)
                     /locus_tag="PKY09_00570"
     CDS             complement(30315..30905)
                     /locus_tag="PKY09_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNB11370.1"
                     /translation="MKRSVVIIGILLVVAFIIYRLFAGTYNSMVTADENVKGKWAQVE
                     NQYQQRMDLIPNLVNTVKGAAEFEKGTLTAVVEARASATQVKVDPDKLTPENIQKFQE
                     AQGQLSTALGRLLMVTENYPVLKANQNFLELQATLEGTERRIAVARKDFNEAAQQFNT
                     YIRMFPQTLLAGMFGFSSKGYFQSDKGANKAPEVKF"
     gene            31085..31354
                     /locus_tag="PKY09_00575"
     CDS             31085..31354
                     /locus_tag="PKY09_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNB11371.1"
                     /translation="MRKTVAIEIIGKVQGVFFRKYTQQTGCRLNLCGFVCNRPDGSVY
                     IEATGSELQIKEFIRWCHNGSPMSVVRQVVVREISVIHSEPFQIQ"
     gene            31396..32919
                     /locus_tag="PKY09_00580"
     CDS             31396..32919
                     /locus_tag="PKY09_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB11372.1"
                     /translation="MSGIKKLAGQTVLYGLSSIVGRMLNYLLVPIYTRIFSPAEYGVV
                     SELYSYSAFLLILFTYGMETAFFNFMQKEDNKNKVYENCISLVSYTTTILVLLMIFFS
                     SPLAATIEYPGKGNYIIWLALIIGFDAITAIPFARLRQQGKALQFALLKIAGIVINIG
                     LNLFFLVGCPVWIKGNDGLLSQLAVTFYSPTVGVGYVFISNVIASGLTMLLLYRQFSN
                     LTWHLDSALIKRMLYYAFPLLIAGFAGMVNETFDRAVYKYLAPDANHALRDLGIYSAC
                     YKLSIIMTLFVQTFRYAAEPFFFAQHDKQDKKETYSKVMTWFVITCSFIFLGVMLFID
                     VIKLFIGEQFRSGIDVVPVLLLANLCLGVFYNLSMWYKLTEKTRYGAYFSIFGAVLTI
                     ILLFVLIPSFGYMGAAWATFITYFAMMIVSFITGQKHYPIPYKVKNDVFYVLLSLMLF
                     SLSVYLQHQLDLPGSVKWIVNIILLFIFPFVVFRMNRGELIKKQNVESQQNTIGRRN"
     gene            32971..33414
                     /gene="dut"
                     /locus_tag="PKY09_00585"
     CDS             32971..33414
                     /gene="dut"
                     /locus_tag="PKY09_00585"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNB11373.1"
                     /translation="MQNNTEVKIINKSSHPLPAYESNSAAGMDLRADIKEAIVLNPLE
                     RALIPTGIYIELPLGYEAQIRPRSGLAAKHGVTVLNTPGTIDADYRGEVKVILVNLSS
                     AAFTINDGERIAQMVVASHHRVVWKETQELSETNRGAGGFGSTGK"
     gene            33497..34228
                     /locus_tag="PKY09_00590"
     CDS             33497..34228
                     /locus_tag="PKY09_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNB11374.1"
                     /translation="MIESLVIIPTYNEKENIQKIIKAVFNLLNTFHVLVIDDNSSDGT
                     SDLVKQLMPQFPESLHLVQRQGKMGLGTAYIHGFKWALERNYQYIFEMDADFSHNPAD
                     LQRLLDACKNGGADVAVGSRYINGVNVVNWPIGRVIMSYYASVYVRLITRMPVRDATA
                     GFKCYKRSVLETLDLSKIKFMGYAFQIEMKFAAWCSGFRIIEVPIIFTDRTEGKSKMS
                     SGIFKEAFFGVIQMKIKSWFSKYTL"
     gene            complement(34312..35400)
                     /locus_tag="PKY09_00595"
     CDS             complement(34312..35400)
                     /locus_tag="PKY09_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNB11375.1"
                     /translation="MNKNNYCVIMAGGIGSRFWPMSRNAFPKQFLDILGTGHTLLQQT
                     FNRFNKICPKENIYIVTNETYIDLVKKNLPQLPVENILGEPLRRNTAPCVSYAAFKIA
                     AKNPDANIVVAPSDHIITKEDAFTEAINKGLSFTAQNDCLLTLGITPSRPDTGYGYIQ
                     LVDEKHNETNKIFKVKTFTEKPNLEMARFFLQSGEFVWNAGIFLWNLKSILKALKEHL
                     PEMYHLFEEGNSVYNKAGEEAFIKTAYSQCTNISIDFGVMEKANNVYVMSADFGWSDL
                     GTYGSLYEHITHDANKNAVVGKNVMLYDTKNCIVNMPHDKLVVLQGLSDYIVVEAENI
                     LLVCRKQDEQQIRQFVNDVKTAKGEKFV"
     gene            complement(35606..39112)
                     /locus_tag="PKY09_00600"
     CDS             complement(35606..39112)
                     /locus_tag="PKY09_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB11376.1"
                     /translation="MLKRFLFILMLFTTTIAMAQTDFRLMFLGKAVLPTENLNSFIQE
                     KVSENDIYNGYYYRIIQFYSVPTQAEKAAIETAGIKLLEYLPKNAFNAAIPIGLNKQL
                     LSGFNVRSVTNQSSEQKLSVSMLGDYPAYAVPAAGMVDVRLQFQKNISIQEAQLIAAS
                     YGKVLSSTEINNMVEVRMPQQSVASVLQQPWLFFIDLTSPPSVKDDLEGRSLHRSNVI
                     NNDLLMGRKYNGSGVAAALADDGFVGPHIDFTGRITNYSTNTGPSHGDMTGGILAGAG
                     NLNPAYPGMANGVQLHVFDIGNYPQIIDAVANYNNLGTVVASTSYSQGCNEYTTDTQF
                     GDQTIHDNGQLEFVFSAGNNNGANCGYGAGGNWGNITGGYKQGKNVIAVANLDALEVI
                     DPSSSIGPASDGRVKPDIAANGRDQMSTDENNTYQVGGGTSAACPGIAGICTQLIQAY
                     KQINSASDAPTALIKACLLNGAEDIGNPGPDYKYGWGRVNALRAVTTIEDARYFTDSI
                     NQGDSLTYSINVPAGTSQMRVMVYWHDVGGSPASSTYLVNDIDMQVTDPSSVSWNPWI
                     LDPTPNAANLNAPATRGIDHLNNMEQVTLDNPAAGNYIVSLKGFAIPQGPQTYYVVYE
                     FRNDDITVTYPMGGEGFVPGHPEIIRWDALKGLGSFDVEYTTDNGTTWNNIATVNQNT
                     LQYTWNVPNNIATTGDARVRVTRGSVSGLSDTTFTIIGIPQNLNVIYSCPDSIKIGWT
                     AVSNAAWYEVSMLGAMYMDSVATTTNNSYVFTNLTSSNTYWFSVRAVMANGNKGRRAN
                     AIEKQPGIFNCPNIPPFVQFSANFNVGCTSKTFTFTDQSSNAPSSWNWTFNPSTVTFV
                     NGTSATSQNPQVQFNASGTYDVTLDATNGIGTSSSTQTAMINILAPSLPPMTQDFQAT
                     AFPPANWTIEPSNINYTWQKSALITGSAGTSTYAARFNNFSYNSSGAEDGLISYEVSL
                     SNAVAALLTFDVAYAQYSSVYEDGLRVDISTDCGNTWVASGYLKIGAALATTGTTTSN
                     FAPSAANQWRNDTVSLSAYLGQDVSIKFVNINDYGNSMYVDNVNLDITTGLTQLNNSV
                     GTLSISPNPSDGKFIIYSNRSSHESYTLTIYDINGKAIAKDEFPANNKSHAVDISNFA
                     KGVYFIKVESQNSVQRLKVISR"
     gene            39264..40076
                     /gene="bamD"
                     /locus_tag="PKY09_00605"
     CDS             39264..40076
                     /gene="bamD"
                     /locus_tag="PKY09_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNB11377.1"
                     /translation="MKNRLSVYFLLMALVMTLGSCSKFNRIRKSGDMNMKYDAAVKYF
                     ENKKYYNALQLFEELIVVLKGTDKAESSYYYYCLSYFYTGDYTSAAYHFNNFAQTFPM
                     SAKAEDALFYNAYCYYLDSPPSSLDQQSSLDAIRQFQLFINKYPTSQRVAECNKYIDE
                     LRSKLEVKAWDNAMLYYKMEEYKSAIVAFENVVKDFPTTKYQEECLFLSLKSAYLYAQ
                     NSIESKKEERYKSTIDHYNKLIEIFPETEYKKEASRIMSAAEEKLKDYHTVN"
     gene            40085..40423
                     /locus_tag="PKY09_00610"
     CDS             40085..40423
                     /locus_tag="PKY09_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNB11378.1"
                     /translation="MNSRNVAQTTVTRDVVDFEKKTENIYESLAIIAKRANHIAAETR
                     EELHDKLSEFASSTDTLDEVFENREQIEISKYYERLPKPTLVAIQDFLDDKIYFRSPA
                     KEENKDSAEN"
     gene            40428..41633
                     /gene="coaBC"
                     /locus_tag="PKY09_00615"
     CDS             40428..41633
                     /gene="coaBC"
                     /locus_tag="PKY09_00615"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNB11379.1"
                     /translation="MLQGKKILLGVTGSIAAYKTPELVRQLVQQGASVKVIITAAAKD
                     FVSPLVLATVSKNEALQTFTSEDHSTWNNHVELGLWCDIFLVAPLSANTLAKFANGNC
                     DNLLTAVFLSCRNTVMVAPAMDVDMFKHASTQKNLEIIAKNNIKIIGPESGELASGLT
                     GKGRMTEPEDIVQEVINYFNPSLPLRNKMAVVTTGPTQEALDPVRFIGNHSSGKMGVA
                     VANCLAAKGASVILISGPSVSLGNLNKSIKVIHVTSAADMLKACKQTQPDANIMVMAA
                     AVADFKPEGFSEQKIKKKSSEGLNIQLVPTEDIAAEMGKLKKEGQLLVGFALETNDET
                     QHAERKLKNKNLDFIVLNKLEETNQVFGSDFNRITIIDKNLVTTPFEKKSKDAVASDI
                     VEKIITLVQ"
     gene            41630..42526
                     /locus_tag="PKY09_00620"
     CDS             41630..42526
                     /locus_tag="PKY09_00620"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNB11380.1"
                     /translation="MKKIFGFIILMMSLNIVQAQELNCQVNVMAPQIQNVDKKVFETL
                     QQSIFEFMNNTKWTSDKYLNQERIECSVTITINERVSNDEYKGSIQVTSRRPVFQTSY
                     NSPTLNFNDDNFQFKYIEYQPLEFSENGSNPNLTAVLAFYANIILGVDYDSFSLYGGA
                     PYFARAQTIVNNMQNAREAGWRAFEGTKNRYWLSENMTNPVFKPYHEMFYQFHLKGLD
                     VMAKKKDDGLSQISESLDLLMSVHKEKPGSLLLQTLFYAKADEIVNIYSGAYPDQKVK
                     VSNLLNEIDPANGTKYSKIMGN"
     gene            complement(42594..42947)
                     /locus_tag="PKY09_00625"
     CDS             complement(42594..42947)
                     /locus_tag="PKY09_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11381.1"
                     /translation="MCREDDYSTEEASNRIRYHGNTPKSINTNTTAMNVRIEVVPNPA
                     SQTTTFNYFIPTIKEQIVLSVFDMSGKELKQFTVSDKQGTIKWDLGNVGSGLYYYTVK
                     DGETKIAKGKITVKK"
     gene            complement(42954..46562)
                     /locus_tag="PKY09_00630"
     CDS             complement(42954..46562)
                     /locus_tag="PKY09_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11382.1"
                     /translation="MKTLTQIAIFMFAVCTTGLSQTTQMNNWYIAPNRINMTVPNPFP
                     AAISPAIGSTTAATALHVANGFYDNTSTGDLLFYIADGTVYDYNNTKIGAIPNGGAEI
                     AVVPFGNNDYCHKKFNIFTTAGGLIYPVALWKTVLDMNSYSLSSLPIDSVPFGSEFGA
                     IAVGKVIDTANNRYLYFLGGSGFINTTGGLIKKLIIHNDGSVSSSVPVYPTSANPNYN
                     AGGEVFSKELELSPDGKWLAWASYALVTHQSGPQYRYHLIELDGSGDYINNSYKKFNI
                     PNYSGNNISGFRGVEFFQPANATKLFMGAGTDGVFYINLPWSNWSNYPTPPPSTDFTR
                     VVNSNTPNNFGFSQIELAHSNNGFMYAVADPGSITNNVGAFNPYANTPQILPSPNSFP
                     LSNPPYDLYNLGGVNPNSTMYTLPDQIDGQDYSLITSSPIPHVITTNTINFPDNSSIN
                     QNATWSYGATNNPLGASTPIYVLNELRVKQNSNLTINGMMFKFSPNAKVIIEPGSTLT
                     LDGGAVLTSDYLEDPCLIPYTWQGVEVWGNQNNQSQNIVPSVVGKLIVNNATIEYAVC
                     GARAQRNYGSYVNLHRGGIIIATAGAKFKNCLMDVEFLPYQNIYNGIEYSNKSYFAGA
                     TFENNIKYVFQATPIHAHIDGCFGIRFVNCDFINFNSQAMFDMLSMGIKSLNANYTVS
                     DGCTFLNLFHAIDAKRTAGNNSITVLGSTFTNNQTGIYVYNINNLKLQSNYFYIGNNF
                     KTGANVQLGISTIFCSGFTIEENHFFRSNPNIATVFKYGISTNSCGQSANQIYKNYFT
                     DMSIGNYSTGINRNPIDTRLTGLQYLCNENTSNDNFDFFISGKGSPFSGIRLLQGTSK
                     ISAGNIFSYTGLTGTFTDINNVTAVALSYYFDQSKAPQYYNSQVAPILAAFSNTCPSH
                     ICQNCNDLLTNGEIQQMTDSYDSSETAFLNLLYSYNQLLDGGNTNILLNQIQQSWSSN
                     AVDLRDHLMGISPYVSADALREAAGQDIMPPAMLLMVCLANPDATRDEALLEFMQYSI
                     VNSLSQYMVDLIRASWDDGTPRTAIENMLADYNSQMAVISDRLLSDLYAKMTLDMDSI
                     IIGDTTDYSRQIIYWLTRAQTLTAKYDLIENYLAANELTSAELVLSSIPVDFTLSDDQ
                     QNAYNDYCYFYSFRNQLQQDSSDLSMLDSTQINQLMSFVQGELSFAKGMAQNALCF"
     gene            complement(46594..47997)
                     /locus_tag="PKY09_00635"
     CDS             complement(46594..47997)
                     /locus_tag="PKY09_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11383.1"
                     /translation="MKKIALFITLLFCNTTLFAQNTFEKIIDTLGSGGANCIQETYDG
                     GYIYGGVCSLNGNDAIIVKLDSIGTIEWVKTYSGPSIEGVLYLEQIPDSGYMVNALYD
                     GGANSKSWLLRLDANGDTLWTKTFSVGIGPTLPPQQNSMASVNNVIYGLSGYYIPPSF
                     MPDIFFISVTGNGFLLSSKVYTTPFSSDGGSIWKTHDGGFIMAGEIGTPSFHSDIYMI
                     RTNTYGDTLWTKSYDLSYHEVAQDIKQTNDSGFVITGYVENTATFQYNIGIIKTNAIG
                     DTLWTKLYGDTISCGGSSIYQTNDGGFILTGVFAGDIYLLKTDSNGDSLWSRRFGQLN
                     TGDFSFFVRQTKDNGYIIGGQGIVNGIGGSYIIKTDSMGNVNSGTGIAEVNNPFKFNI
                     YPNPSSGNFTIQVKGLPQKNTELKIYNLMNQCIYSCRLSNNVKEQIDLSHLPNGMYVA
                     TIMVNNKMVSQKISIYK"
     gene            complement(48233..48622)
                     /locus_tag="PKY09_00640"
     CDS             complement(48233..48622)
                     /locus_tag="PKY09_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11384.1"
                     /translation="MREFVFLILIGAVVLFSAFKTKQDGKDYGQCLQKVNKKFGAETL
                     SCPASTKPYKVWFVNTCNDTLAVKLAVQEKSKRWRTFTRTQLLPGDTISGYACTGVGK
                     YLYYTKQYKDNSVDLPSDEVIDSSNQE"
     gene            complement(48628..49182)
                     /locus_tag="PKY09_00645"
     CDS             complement(48628..49182)
                     /locus_tag="PKY09_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11385.1"
                     /translation="MKIQNLLNNAKILFIVLLSGFCFQANAQVDSADFKMRIDSLMNQ
                     GKIKFNNGKYQEALLDYTYVANMDVANVNAILQQGFCNTILKKYTEAIGNFTDVIFYE
                     PNHEWAYVSRGSAYNKIEKYDKAIVDFNKALELKPEDAEAYNNRGFAKKMLGDIKGAC
                     EDWNKSKKLGNEEAKIILKNNYCK"
     gene            complement(49157..49639)
                     /locus_tag="PKY09_00650"
     CDS             complement(49157..49639)
                     /locus_tag="PKY09_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11386.1"
                     /translation="MVYDKINKEFSKRRKFFLLLLILSVFSPAIKAQENITVQQTLDY
                     INQKFGGKYVVSINYGVLIASYFDGETKYREDQSSIKELDANKVYYNAKAGMLLINCT
                     NPQKKCVTREFLGKRLFYSRISFPVSYGQKTNDGLVKAFQHLIRLINDKNYENSEPFE
                     "
     gene            49732..50931
                     /locus_tag="PKY09_00655"
     CDS             49732..50931
                     /locus_tag="PKY09_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11387.1"
                     /translation="MFGIGQRIHLVPVGLFLTIFMFIYGDSTAQTKKTLTINAKFKID
                     GGGNLSDAYMVLQRDGQSIETLPGQSSYTLNLEIGADYILSFNKPGYITKKININTTG
                     ADEERIEQGFESYPFTVVLMKQYEGVNIVVFNQPVAKITYSKRFDEFDYDTDYTKSIQ
                     SAIQKAEDDLKKAEKEFKKNPPKPAKDSTLVANKPKAEEVKPQKEKTKTAPQEEPVAV
                     KEKRKDDDAKNESKNATKNALATGGQDQPLKPTKGGGSDDPEGNLDREKGADQKSDID
                     LTVGNDAIKNKISLTKGSDQSPVEAPAESHPVVIKAAEKPLPEKPKEVVVHTAPEPPE
                     NVSVRREQIAEDKKTVYNVWVTKGNKTVLYSKVVYAWGGVYFFRDLNTSISENMYKQA
                     DAGLVNF"
     gene            51054..51242
                     /locus_tag="PKY09_00660"
     CDS             51054..51242
                     /locus_tag="PKY09_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11388.1"
                     /translation="MLNTYFCMNLFKPQNDSQVSLLEDIKTLRSIKGNTSRFTILRSV
                     AKTRKHTRTVYPDGWRFE"
     gene            complement(51255..52553)
                     /locus_tag="PKY09_00665"
     CDS             complement(51255..52553)
                     /locus_tag="PKY09_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11389.1"
                     /translation="MKKELTIAVVLLMAFFNATAQTTCTVQASVTYSVGSQGRCAGKD
                     VILESFPNNFTTTYQWTCPNNNCPPNFNYNASNGSFISAKDLSNNGYYVVTATDSSGC
                     VAVDSVLVDSYEIPFAGVSIQALGVCPGDTIIFCATDTSTTNTIISYLWDNGDTTECV
                     YGYQSTMNYPCPPCIVTNQLGCASTNSIIWNVMAVEDFPDTTININGSLTFCHGDSVE
                     LCSIAGSLTSTFLWSNGSALNCTTIDSSGNYFVVITNLSGCSRSSAPISVTVYPEQMA
                     QLTFSAANDTLLSNINGSNFIWYFSVDGITYSDIPSAFNYMPVSTNGYYFLTFTDTNG
                     CMAFSDTISRTVGLADLQSSLNLYPNPATDEIVVDLTQLNILTNATVEISDMYGRKII
                     SLPINKNIKHKIDISRLTKGLYTLHINANTFSNSRLFVKQ"
     gene            complement(52647..53789)
                     /locus_tag="PKY09_00670"
     CDS             complement(52647..53789)
                     /locus_tag="PKY09_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNB11390.1"
                     /translation="MKVYFDNAATTPLRQEVIEAMLPVMQKVYGNPSSIHSFGREMRS
                     AIEKARKTIATLLKVAPSEIFFTSGGTEANNMAIRCTIHDHKITHAISSPIEHHAVTH
                     TLEDLEKEGIIKLSWVKLNDKGEADYEHLEELLKNNPRSFVSLMHANNELGTVNNIYL
                     IGELCKKYNAIFHCDTVQTMGHIPLDISNLNVDFFTCAAHKFHGPKGVGFLYINHKLK
                     IHPLITGGAQERNMRGGTENAYGIVGMAKALELAYAELDEERKHILGLKEYMKQQLLD
                     EIPGVSFNGDVSENSLFTVLNVSFPPFEGKDMLLFNLDISGIAASGGSACSSGSEIGS
                     HVLRGINSDPERPAVRFSFSRYNTKAEIDFCMDKLKALFVVKAAVK"
     gene            complement(53820..55226)
                     /gene="glmM"
                     /locus_tag="PKY09_00675"
     CDS             complement(53820..55226)
                     /gene="glmM"
                     /locus_tag="PKY09_00675"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNB11391.1"
                     /translation="MTLIKSISGIRGTIGGKSGESLTPVDVVKFSAAYATWLAAQFPV
                     KTKCKVVLGRDARISGPMIHNLVCGTLTAMGLDVIDVGLSTTPTVEMAVTFHQAHGGI
                     ILTASHNPKQWNALKLLNSKGEFISAADGETLLKIAEQESFEFANVNQLGTVTTDTHA
                     IDKHIEAILALPLVDTKAIAAKKFKIVIDCVNSSGGIALPPLLKALGVEEVIELYCTP
                     DGQFPHNPEPLPEHLTELSKTVIKSKAHLGISVDPDVDRLALVCEDGEMFGEEYTLVA
                     VADYVLKNKKGNTVSNLSSTRALRDVTEKAGGSHFASAVGEVNVVEMMKAKNAVIGGE
                     GNGGVIYPDIHYGRDALAGIALFLTHLAKYGNTVSMLRSSYPDYFISKNKIELTPEIN
                     VDGVLESIQEKYKKHKINTIDGLKIEFDKEWVHLRKSNTEPIIRIYAESDSITTANNL
                     AEKIISDIKELISHPAKV"
     gene            complement(55342..56283)
                     /locus_tag="PKY09_00680"
     CDS             complement(55342..56283)
                     /locus_tag="PKY09_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB11392.1"
                     /translation="MPKEILLIDTFHETFIPKLTTLGFNCTEGYEWSLEQLQQTIGNY
                     EGIAIRSRIPLNKDLLQHATNLKFIARGGAGMENIDVAYAVSKNIKCLNAPEANCDAV
                     GEHALMMLLALLNKLLKSDKEVRNGIWLREENRGTELQGKTVGIIGFGNMGSSFAKKL
                     TGFEVNILACDPYKTIDAAEYPFIKQVSVEQLQEQSDIISLHVPLTAETKYMINDKFI
                     SQCKKEIYIINTARGKCLNTADLVKHLKTGKVKGACLDVHEYETSSFERLETLPEPFE
                     YLIQSDKTILTPHIAGWTHESNRKIAEVLAKKIGQLF"
     gene            56765..56962
                     /locus_tag="PKY09_00685"
     CDS             56765..56962
                     /locus_tag="PKY09_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11393.1"
                     /translation="MHVLDILLLGGAVVCFVLAYIIASRPARELRQSSKSKSKWNHVN
                     MDSASSVHSPEMMKYVHDKTG"
     gene            complement(56959..59055)
                     /gene="recG"
                     /locus_tag="PKY09_00690"
     CDS             complement(56959..59055)
                     /gene="recG"
                     /locus_tag="PKY09_00690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNB11394.1"
                     /translation="MQSYFQKDIEFVKTVGPQRAAVLRKELEVHNVSDLLHHFPFRYV
                     DRSKFYTIAQLNDDLPYVQIKGVITSLTAFGNKRTTRLQAVFKDASGSMDLVWFQGIT
                     WIRDSIKAGTSYVVFGKPALFNGKWSIIHPEIEELEKFTAQPFKGFQPFYYSTEKSKV
                     KGLDSKGIMRIMRMALKDLPPTIEETLSSQLIKTFNLMPLRDALIQIHFPENEDLLRQ
                     ARFRLKFEELFYIQLKLLLNKQKRSSTQSGRMFTEVGYLFNTFFNDHLPFELTQAQKR
                     VLKEIRKDTISGKQMNRLLQGDVGSGKTVIALFSMLLALDNKQQACLMAPTEILAAQH
                     FQTITSLLSNLPVNVELLTGSFPESKKKAIIERISSNETHILIGTHALIEDRVKFNDL
                     GIVIIDEQHRFGVEQRAKLWMRQQPPHVLVMTATPIPRTLAMTLYGDLDVSVIDELPA
                     GRKPIKTMHLFESSRLRVIGFMKEQIKLGRQIYIVFPLIDESEKLDLQNLMAGYDSIQ
                     RDFPLPEYFVSMVHGKMKAKDKEQEMERFVNGKTHIMIATTVIEVGVNVPNATVMIIE
                     NAERFGLSQLHQLRGRVGRGAEQSFCILMSGNKLSADARERLKTMVQTNDGFQIAETD
                     LKLRGPGDITGTQQSGLLNLKIAELAKDGAILEHARNAVINILNEDPQLNSEENKIFY
                     RHLHQLREQPNWSKVS"
     gene            complement(59310..61619)
                     /locus_tag="PKY09_00695"
     CDS             complement(59310..61619)
                     /locus_tag="PKY09_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNB11395.1"
                     /translation="MKRFYILLLLISTFTLSQAQQPVLVSITPDTVALGQTLSATITG
                     ANTLFQNSSTIGHLYLGGVTIPMNNISVIDDEHFTGNFQIPSTGIPIGFYDLYVLNFD
                     PFQGLSYDIILPSAVRVGSPDGYITGQVYEDLNNNGVKDATESGIAGSSVKIMPQNHV
                     YTTDANGNYTIAVTNGSYTVSWVNNPNIIFLFSTSSPNHSVTINNNNSSGNDFGVKRG
                     LISVSPTVLVQGQTVTLTIVSDSLFQTSANTYGNIQSASIVKHGTGNHYSANLTTQVQ
                     ILDEVTAEITLNIPTNALPGLYDLSIQTISPFGGRHILPFSNAISPAQSIVNGKIYFD
                     TNSNGVFDAGDFPIPNQYVTLMPDSITGISDANGDYKIGSSNGSKTLTYVPTVAYTLT
                     STPATYNFSVNNSIASNKDFGFQTGTPFYEGSLIISGLPRCNTQQPYYVTFRNQSNTA
                     VNGVLGIVCSGNITVASATPDFTVGDTLFWNITNLNLFSTFSKNVVLNIPGAGSIISV
                     TGIIRLTDGSNVVLNHSRTNTQMVLCSFDPNDKAVTPPGVQSQNYTLMNERLYYTVRF
                     QNTGNDTAFTVQIRDLLDPELNWQTFRVEDYSHTVTPTLNMKTGALLFTFNNILLPDS
                     NTNEPASHGYVIYSIEADSGLIAGTMINNTADIYFDLNEPVITNTTLNTMVYVIPVGL
                     NELAPEKSDVIVMPNPASEKVLLKFDNISGSVYNLSVYSMAGKIVHHSKTANGEVLIS
                     LDKFNAGLYIYELTPNSSGKAYRGKIVVK"
     gene            complement(61869..62063)
                     /locus_tag="PKY09_00700"
     CDS             complement(61869..62063)
                     /locus_tag="PKY09_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNB11396.1"
                     /translation="MKTGKVKFYNNSKGFGFIVEDEGGRDLFVHQTGLIDRIADNDLV
                     TYEVVQGKKGLNAVNVKKQQ"
     gene            62163..63782
                     /gene="ade"
                     /locus_tag="PKY09_00705"
     CDS             62163..63782
                     /gene="ade"
                     /locus_tag="PKY09_00705"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNB11397.1"
                     /translation="MASYSGNIIDIFNRRIFPGTVHFSNGIITEITEDNAVYDQYLLP
                     GFTDAHIHIESSMLVPYEFAKVALIHGTVATVSDPHEIANVCGMKGVEYMIENAKDAK
                     LKFNFGAPSCVPATSFETAGATLNADDVAQLLARPDIHYLSEMMNYPGVLFGDQEVMK
                     KIKSAHRMNKVVDGHAPGLRGDDARTYIAAGISTDHECFTLDEALDKVNAGMKILIRE
                     GSAARNFEALHSLITSHTAMTMLCSDDKHPDELLHGHLNQLVVRALKNGHNLFDVLQC
                     ACVNPVLHYGLSVGLLREGDSADFIVINNPEEFLVSQTWIDGECVAKDGRTTLTNRSH
                     NSINNFHAWNVTVQDIEVKKTSETVKVIEALDGQLITNCIQAKPLARDGQWVSNPEGD
                     ILKIAVINRYSKQKPVVGFIKNFGLKNGAIASTVAHDSHNIIVTGVGDADIVEAINLL
                     MQSGGGLSAVSGTDKKVIALPVAGLMSDKSCEEIGRDYAAIDSMAKQIGSTLRAPFMT
                     LSFMALLVIPHFKIGDKGLFDAAKFELTTLE"
     gene            63804..64079
                     /locus_tag="PKY09_00710"
     CDS             63804..64079
                     /locus_tag="PKY09_00710"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNB11398.1"
                     /translation="MLLLQKNTMWQEVNNSLERSFVFHDFKQAFAFMTQVAFIAEKMN
                     HHPYWCNVYNKVTIKLNTHEAGSVVTEKDHKLAAAIDVVFNQINQKV"
     gene            64076..64450
                     /locus_tag="PKY09_00715"
     CDS             64076..64450
                     /locus_tag="PKY09_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HNB11399.1"
                     /translation="MSRLKKFFIWVVVLAIIGTALYFLAGSYTYSEGTQTGYLVKFSR
                     QGYVFKTYEGELNLGGMNAESNSVVNNLWHFSVDEDDKAAIDSLRVSEGRVIKVFYRE
                     IVRNYAWQGETPFFVYKVEKVK"
     gene            complement(64455..65024)
                     /locus_tag="PKY09_00720"
     CDS             complement(64455..65024)
                     /locus_tag="PKY09_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013685391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11400.1"
                     /translation="MQDIQPFYNWVDLYSSAEDELSPFYGRVYSEFEYSNTIYNYFIH
                     PQWDDFGSPTLYIKIIYCDYEKSFAIIELMGEWNDAINNDIMTLKRDVIENLMEHGIS
                     RFILIGENVLNFHNSDDCYYQEWFEEVNETGWIAAINFRDHVLSEMQRANIDYYLLFG
                     GELDNLNWRTYRPSQLMAKIDSIIRHRLG"
     gene            complement(65077..65901)
                     /gene="murQ"
                     /locus_tag="PKY09_00725"
     CDS             complement(65077..65901)
                     /gene="murQ"
                     /locus_tag="PKY09_00725"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNB11401.1"
                     /translation="MNKKSESLRTTEKTSHYNHLEKMSIQQLLNNINKEDHTVPRQIK
                     KNIGAIEKLVKVITTRMKKGGRLFYIGAGTSGRLGIVDASECPPTYGIEHGKVIGLIA
                     GGDAAIRKAVEFAEDDYSGGWNDLKKFKINKKDVVIGIAASGTTPYVVGALEQCRKKK
                     ITTGCIVCNSASPVAKQADYPIEIITGPEFVTGSTRMKAGTAQKLVLNMISTAVMIKL
                     GRVKGNKMVDMQLTNNKLVSRGTRMIMDELKVPEKKAALLLKKYGSVRAAIKNQKA"
     gene            complement(65894..66736)
                     /locus_tag="PKY09_00730"
     CDS             complement(65894..66736)
                     /locus_tag="PKY09_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB11402.1"
                     /translation="MTEQKKKFIDVSEVIRSKNPQLYRLLPGFIVSYIKRIVHETEVN
                     AFIDEHGHKTSFEFVDAIIENFRVKVEVYGQENIIKTGRCVYASNHPIGGLDAMALMQ
                     ALGHYRRDLKFIVNDILLQLNNLKDLFIGVNKHGKNSSQSIADIDALYASEMATLIFP
                     AGLVSRKQGKEIKDLQWKKSFVVKAKKYNSPIVPVYIDGRNSNWFYNLANFRKRIGIA
                     ANIEMLYLMDEMYFQRGRTISITFGKPILPQDLSGTDDGKTAEKIKEHVYELKHNRQS
                     VFHE"
     gene            66950..67417
                     /gene="mraZ"
                     /locus_tag="PKY09_00735"
     CDS             66950..67417
                     /gene="mraZ"
                     /locus_tag="PKY09_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNB11403.1"
                     /translation="MINLFGVYESTVDAKGRVMLPSPYRKTLEKVLEEGFIIKQNVFR
                     KSLEFFPLQTWNEISQHVGKLNTFLKANQELITLFYYGVRSVELDNNGRFLIVKDHLT
                     YAGIKKDIVLSGANDRIEIWDKRAYHKYIKDNSDNLERLMEERLGGKLPENGQ"
     gene            67407..68321
                     /gene="rsmH"
                     /locus_tag="PKY09_00740"
     CDS             67407..68321
                     /gene="rsmH"
                     /locus_tag="PKY09_00740"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNB11404.1"
                     /translation="MDSKAYHIPVMLPECIEGLKIKADGIYVDVTYGGGGHSKAIASL
                     LTTGKVIAFDRDADAVKNSLTTNKNLIVIHSSFSNITTELNSIGITSVDGVLADLGVS
                     SHQFDEPQRGFAFRFDSALDMRMDVRQQITAATILNTYSTKELQKMFSLYGEVRNAAT
                     VARQIVSFRQHATLATTADLKKCVSHLVPKSDENGFYARLFQALRIEVNQELKELELL
                     LQQLPNLIKAEGRLCVMSYHSLEDRLVKNFIASGNVQGVVEQDVFGNITSKTFTAINK
                     KPIEAKEEEKINNPRSRSARLRVAEKIK"
     gene            68323..68712
                     /locus_tag="PKY09_00745"
     CDS             68323..68712
                     /locus_tag="PKY09_00745"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNB11405.1"
                     /translation="MNTIRNTEEKKEKEKPNGSKIKTARKFLSMLNVIGLIDKRMLLR
                     MLPFAFFLMGLALVYIANSYVAEKTIREIDRTSRDIKELRSEYISVKSELMFKSRQSQ
                     VAKEVLPMGIKQLTVPPKKIVLKTAKD"
     gene            68719..70818
                     /locus_tag="PKY09_00750"
     CDS             68719..70818
                     /locus_tag="PKY09_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNB11406.1"
                     /translation="MADIKKDILLRVYFVYALIFLFAMAIMGRVFQLQYVEGDYWKQR
                     SDSLTLAYKTIEPSRGNIFSADGSLLATSVPLYDLRMDMKAESITDKIFNAGIDSLAL
                     NLAALFQDKTKDQYKRMLREGRRDGERYLLIKRNVSYAELKKVKTFPIFNKGRYKGGL
                     IVEQKNMRQKPFKELASRTIGFKIGNVQPVGLEGAFDHSLRGESGKRLMQKISGNIWK
                     PLNAEDEVEAKDGCDIYTSIDLNIQDVAQQSLYNQLVKNDADAGCAILMEVATGEIKA
                     IVNLKRADSGYYREDYNYAIGYASEPGSTFKLVSMMAGIEDGYIDLEDTVDATNGRTS
                     YAPGLIMRDSHEGLNKITAKHAFEESSNVGVSKLIQKYYRKNQQAFIDRVHKMHFGDK
                     LNIQIAGEATPFIKSTTSKEWSKISLPYMSIGYETMVTPLHTLTFYNAVANNGTMVKP
                     LFVKEIRYRGHTVKEYKPQVVADSICSKSTIAKARELLEGVVQNGTGRKLKDCVYAVA
                     GKTGTAQMANNKGGYKGSKIRYQASFCGYFPADKPAYSCIVVVYNPSTAGYYGGEVAL
                     PVFKDIADKVYATNLDMHKGLDVQMAALPKIKTGLTKATIAACSNVGVDVKTVGTASK
                     WSSVDKRESVAALKPVSLKDNTVPNVEGMGLRDAIYMLETNGLQVKVVGRGAVVKQSL
                     QAGTRIYKGQVITIELS"
     gene            70818..72281
                     /locus_tag="PKY09_00755"
     CDS             70818..72281
                     /locus_tag="PKY09_00755"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNB11407.1"
                     /translation="MQLLKEVLYKAGTEEVIGNTNVAVYGISINSREVKPGEMYVALR
                     GTNVDGHAFILDAIEKGATSVLCEVMPDLIQAGINYIKVKNTYLALSEVCANFYHHPS
                     SSLKLVGITGTNGKTTTATLLYKLFRQLNIRCGLISTVENRVNDTVIPATHTTPDPIK
                     LNQLLSMMVLEECEYCFMEVSSHAVVQNRIGGLEFAGGVFTNITHDHLDYHKTFDEYL
                     KAKKKFFDGLPESAFALTNTDDKNGTVMVQNTHAAKKTYGLHGIADFRCRIIENNFSG
                     LLLQIDNTEVLCKLIGAFNAYNLLAIYATAVSLGLDKQRVLTALSMLESVNGRFDYVV
                     SGSGITGIVDYAHTPDALNNVLSTINEIRTRNEKVITVVGCGGNRDTTKRPVMAHIAC
                     EMSDKVILTSDNPRNEDPQTIIEQMRAGVPAHLSAKVMAITDRNEAIRVACNLAAAGD
                     IILIAGKGHETYQEIKGVKHPFDDKKILQENFNNLHA"
     gene            72281..73537
                     /gene="mraY"
                     /locus_tag="PKY09_00760"
     CDS             72281..73537
                     /gene="mraY"
                     /locus_tag="PKY09_00760"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNB11408.1"
                     /translation="MLYYLFEYLDQSTDLPGMGVFKYISFRAALAIIFSLLFSFILGR
                     TIIAYLHKKQVGETIRDLGLDGQVQKQGTPTMGGLIILSAILIPTILFAKLLNVYIIL
                     MLISTVWLGAIGFLDDYIKVFKKDKKGLAGKFKVLGQIGLGLIVGSVLYFNEHVVVRE
                     KAIRSAVTWHHDNTLVDDLQSQEGVLFSTTNIKSTKTTIPFFKNNEFDYATLLGWLGD
                     GYKNLAWLIFIPVVIIIVTAVSNGANITDGIDGLAAGSSGIIGLALAVFAYVSGNTVF
                     ANYLNIMYIPNAGELLVFMSAFIGACIGFLWFNAYPAQVFMGDTGSLALGGIIAVFAI
                     AIRKELMIPVICGIFLIENLSVVMQVSYFKYTKKKFGEGRRIFKMSPLHHHYQKLGYH
                     ESKIVTRFWIVGIMLAVLSLVTLKLR"
     gene            73542..74885
                     /gene="murD"
                     /locus_tag="PKY09_00765"
     CDS             73542..74885
                     /gene="murD"
                     /locus_tag="PKY09_00765"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNB11409.1"
                     /translation="MSKNKRIAILGAAESGVGAAILAQKQGYDVFVSDLSRIADNYKT
                     HLTERNIQFEEGCHTDSLILNADEIIKSPGIPDKAAVIKKIKNANINIVSEIEFASRF
                     TNAKMIAITGTNGKSTTTLLTYHILKKAGVNVGLAGNIGKSFAWQVAEEKFDHYVLEL
                     SSFQLDDMYTFKADIAVLLNITADHLDRYEYSIQNYIDSKFRIIQNQTAEDAFIYCFD
                     DAVIKTEVLKRNPLATCYPFSIRQDEGMSAYVNDNNLYLNIHNNLFTMSIFELALQGK
                     HNLYNSMAAGVAARILDIRKESIRESFADFRNAEHRMEFVNTVHGIDFINDSKATNVN
                     SAWFALESMQKPVVWILGGVDKGNEYEMLNDLVKEKVKAIVCLGKDNKKIHEAFGSIV
                     SDITDTLSAQEAVSVAYQKGKKGDVVLLSPCCASFDLFKNYEDRGRQFKAAVKAL"
     gene            74904..76061
                     /locus_tag="PKY09_00770"
     CDS             74904..76061
                     /locus_tag="PKY09_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNB11410.1"
                     /translation="MNWIETYFKGDRIIWMVVILLSLMSLMAVYSSTGTLAYKYQEGN
                     TEYYLFKHGIIVFLGFGIMYFTHLVKYTFYAKVSRIAIFLAIPLLLLTLLTGTNLNEA
                     SRWLTLPVINLSFQTSDLAKIALLLYVARSLSKKQDNIKDFKSAFLPVILPVCIICGL
                     ILPANFSTAAVLFSTCLVLMFFGRINFKYILSLVGIGILGLGLFLMIASFSGYEGRIG
                     TWKKRIENFSNGNDQGNYQTQQAKIAIASGGIFGKGPGQSTQRNFLPHPYSDFIFAII
                     IEEYGFMGGLVIVVLYLLLFYRAVVIVRKSPFAFATLLAIGCAFSLVFQAMINMAVAV
                     NLFPVTGQPLPMLSMGGTSIWFTSIAVGIILSVSRNIEQEELKTGVVHAAA"
     gene            76048..77190
                     /gene="murG"
                     /locus_tag="PKY09_00775"
     CDS             76048..77190
                     /gene="murG"
                     /locus_tag="PKY09_00775"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNB11411.1"
                     /translation="MQQPDIKVIISGGGTGGHIFPALAIANAIKSARPLAEILFVGAK
                     GRMEMEKVPAAGYKIEGLWISGIKRELTIDNLSFPFKVLSSISKAMHILKEFKPNAAV
                     GVGGYASGPLLYAASLKKIPCLIQEQNSYPGITNKILAKRVQKICVAYEGMDKYFPAS
                     KITLTGNPVRENVLMLDGKFDEAMSFFNFNLSQKTLLIVGGSQGARSINKSILAGLEK
                     LKNAGVQLIWQTGKLFYEEAQRAVAEAGYEQARVFDFIARMDFAYAAANLVVSRAGAS
                     TVSELTLTGKPSVMVPLPTAAEDHQTKNIEAMVKKDAAVLVKDGDALQHLVDTALKII
                     NDTDRLKQLSANTAAMALPHAAQKIADEVIAMATQHAAQKLKQATA"
     gene            77193..>77493
                     /locus_tag="PKY09_00780"
     CDS             77193..>77493
                     /locus_tag="PKY09_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNB11412.1"
                     /translation="MRFDTITHVYFVGIGGIGMSALARYFKAIGKQVAGYDRSQTVLT
                     DELINENIDVHFNDDEQTIPLAFRDTNRKNNILVVYTPAIPKNHSELNFFRQQEFT"
BASE COUNT        23883 a        13717 c        15161 g        24732 t
ORIGIN      
        1 ggggctttga aacagccgtt agaattattc cctacatggt gggcatgctg gtggcggtga
       61 gtatgctcag aaccagtggt gcttttgatt taattataga tccggtaaaa catcttattg
      121 caatttcggg catggatacc cgttgggccg aaggcttacc tactgctttg ctacgtccat
      181 tcagtgccgg tggtgccaga ggttttatga ttgatgccat gcgcactttc gggccagatt
      241 cttttattgg taaattagtc agcgttttgc aaggctcgag cgagacaacc ttttatgtgg
      301 ttgcattgta ttttggttct gtatcggtaa aagatacacg ctatgctatt cccgtaatgc
      361 ttcttgctga tttggcaggc gttatattct caattgcaat ttgttattta ttgtgggctt
      421 agtctaatag gttagttgtt agtggattac aataataaag gtcaggtttt atttaagttg
      481 attttaaggc caaaaataga ggtatagtaa tcaagttttt ttatactttc gcaacctcaa
      541 aaattttaac cgtagtattt tatgagtcag atagtttatg tagttccggt gttgggtttg
      601 ttggccctgg tttatatgtt tataaaagcc tcgtgggtag gcaagcagga tgccggtgag
      661 cagaatatgc aagaacttgc cagtcacatt gccaatggtg ctatggcatt tttaaaagca
      721 gaatggaaaa tattgtccta ttttgttgtt atagccggat tgcttttagc atattcagga
      781 acattacatg gcacagaaga atatccggtt cattcaagcc cgattatagc gattgctttt
      841 gtgataggtg cagttttttc tgcagtagca ggctatatag gaatgaacat tgctacaaaa
      901 gcaaacgttc gaacagcaca ggctgcacgc agtagtcttt caaaagcatt aaaagtctca
      961 tttaccggag gtgcagtaat gggcctgggt gttgcagggc ttgccgtgct gggattgggg
     1021 tctctattta ttgtttttta tcagttgttt gttccagcag gtgcagctat caccggagta
     1081 gaaatgaaaa aagctattga agtattagca gggttttctt taggtgcaga gagtattgca
     1141 ttgtttgccc gcgtgggtgg cggtatttac accaaagctg ccgatgtagg tgctgacttg
     1201 gttggaaaag ttgaagccgg aattccggaa gatgatccac gtaacccggc tactattgca
     1261 gataatgtgg gtgacaacgt aggtgatgtt gccggtatgg gtgctgacct ttttggttcc
     1321 tatgttgcaa ctattcttgc taccatggta ttaggccagg agattgatgc ttctgctgat
     1381 aaatttggtg gactttcgcc tattttatta ccaatgctca ttgccggaat gggaatcgtt
     1441 ttttctattg taggaacgtg gtttgtgaga attaagaatg aaaacgacaa tgtacagaat
     1501 gccttaaaca tgggtaactg gtcttcaatt gtacttacag ccattgccag ttattttgcc
     1561 gtgacctatt tacttccgga aaagttagtt attcgtggtt ttgaattcac acccatgaat
     1621 gtttatttag ctattctcgt tgggcttgtt gtgggtgcat taatgagcat catcacagaa
     1681 tattatacag caatcggaaa gcgtcctgtt ttgtcaatcg ttaagcaaag ttcaaccggt
     1741 catgccacaa acattattgg tggattagcc gtaggtatgg aatcaactgc agtgcctatg
     1801 attgtacttg caggaggtat ttttctttcc tattcattag ccggtttata tggcgtagcc
     1861 atagcagcag ccggtatgat ggctaccaca gctatgcagt tggctattga tgcctttggt
     1921 cctattgcag ataatgccgg tggtattgcc gaaatgagcc agcttccaaa agaagttcgt
     1981 cagcgtacag ataatcttga tgctgtagga aacactactg cggcaacggg taaagggttt
     2041 gccattgcat cggcagcatt aacatcatta gcactgtttg cagcctttgt tggtgttgct
     2101 ggtatttcgc atattgacat ttataaagca aacgtacttg caggcctgtt tgttggcgcc
     2161 atgattccat ttatattttc ttcattggct attgctgccg taggtcgtgc ggcaatgagt
     2221 atggttgagg aagtccgcag acagtttcgc gaaataccgg gcatcatgga atataaagct
     2281 aaacctgaat atgagaagtg tgttgctatc tctacagaag cttctatcag agaaatgatt
     2341 gctcctggag cattagcatt aattgtgcct gtagtagttg gatttatttt tggtcccgaa
     2401 gttttaggtg gtgtgttggc aggagttaca gtttccggtg tgctgatggg aatgttccaa
     2461 agtaatgccg gtggtgcatg ggataatgca aaaaaatctt tcgagaaagg tgtagatata
     2521 aatggtcaga catattacaa aggttcagaa ccacataaag catcggtaac cggtgacacc
     2581 gttggtgatc catttaaaga tacatcaggg ccatcaatga acattttgat taaattgatg
     2641 agcattgttt cattgattat tgcaccacat attgcatcaa ttggtactca agattcgttt
     2701 agtaacaaga gcgaagcaaa agttgatgct tcagctgtta cctcaacggt aaatagtaca
     2761 cctgaaatgg accttgctgc tatggccgga acaattagtc ttgacgtaag tcattcctgg
     2821 atgcagtttg gtatctctca tttcctgaca accgccaatg gtactttagc tgttgataca
     2881 ggttatatta atatcactaa tgacttggca acaagtacaa tttttgttca gttgaatgta
     2941 aaatctattg acacaaaaaa cacaaagcga aacaatcatc ttttgagttc cgactttttt
     3001 gatgcagaaa aatttcctgt tattgttttt aaatcaaaca aaattgaaaa ggctgcagaa
     3061 ggttcagcat tccgttatat tgcaaaaggt gagttaacaa ttaaagatat tacaaaacct
     3121 gtagaactaa ggtttaacta catgggggca ttgccaaatg aatatgcagc taaagatgcc
     3181 catacttttg taggcgaagc agatcttgac agaattgatt ttagtgttgg tgaatcaggc
     3241 ccgacagtag gtaataaagt aaaagcaact ttctctgttg aaggctataa aatttaatta
     3301 aagcgttaat gtgaattcag aatggctgtc tcaaaaggat ggcctttttt attaactcta
     3361 aaaaatagtt attcgattac cacccactgg caagcacatc ggcaatgtgc ataaattgca
     3421 atgaggaaac ttcattgtct gcaatacttt ttaagtgcat cagacaggta tagtcagttg
     3481 agataaccgt actagctcct gtttgaatca attcctgaat ttttctttta ccgatatcta
     3541 ctgatatggc ttcgtactta tctgtaaatg atggagagta accacagcaa ctgtcagtgc
     3601 tttcaatcca ttcaagacct tttactttac tgagcagggt gcgaacagaa tcttttacag
     3661 agagttcatt aatggctttg caactgtcaa ggataactac ttttccatta aatcgtgcac
     3721 caacatcggt tacattcagc acgctgacta aaaaatctgt aaactcataa atgtttttct
     3781 gaacactttt atattcatta tgcaacgatg agttgtgaaa catctgcgga taaatatttt
     3841 ttatcattcc ggaacaataa gcaccgggac aaacaatgta tcgttcattc tgaaattctt
     3901 taatcaactt ctcgccaaca gctttacatt ctttctgata accctcttca aatgctgttc
     3961 tgccacaaca tgtctgttcc acattatagt ttactccaca tcctaatttc tcaataattt
     4021 tcacaacatt attggctgtt tcaggaaaaa cctgatccat atagcaggga ataagtaaat
     4081 caactatcat tctttgtttg caaatatcct ataaaaaatc aggtgctttg taattttaaa
     4141 tttctgatgc taagtaatga aaaaagtccc actacaaaaa aagtcatcaa aactaaaatt
     4201 gaatttcgca ttgaacccgt tagctcttca atatatccgt aagctactgt tcccagtacc
     4261 aaaccaattt tatcgcaaac atcataaaag ctgaaatacg atgcattgtc ctgtgttttt
     4321 ggcagcattt tactataagt tgaacgtgac aatgactgaa tgccacccat aatcatacca
     4381 acagcaaatg ctaatacaaa aaactgtact gatgtagtta caaaataggc agcccagcaa
     4441 ataccaatcc aaatggtaac accaatcaac aaagctttaa tatttcctgc ttttgatgaa
     4501 agagaagcaa ataagtaagc accggcaata gcaacaaact gaataatcag aacagtaata
     4561 attagttcat catcgcgcat gtgcagttct ttggtgccaa acaaactggc aacatacatt
     4621 acagtttgca cacccatatt gtagaaaaaa aatgaaatca aaaatcgttt taacatgggc
     4681 aatgactgaa gttgtttcag tacttttaat aattcattaa aaccttttgt cagtagttgc
     4741 tggcttgtgt tgtgcgtact tgttttttca ggaagatatt tgaatgtgaa aagtgcaaat
     4801 ccaaaccacc aagctcctgt cattaaaaaa gatattcgtg atgccatgcc tgaattggta
     4861 ataccaaacc acgatggatt taaaatcatt gccagattaa aaatcaaaag aagagagctg
     4921 cctatataac ctaatgcaaa acctcgcgca ctcactttgt cctgcatgtc ggcagttgca
     4981 atttcgggca aaaaggcatt gtaaaagacg atgcttccgg cccagccaat gctggcaagt
     5041 ataaatgaaa tgattcccat gtcgagagaa gagaggtctt taaaaaaata aagtgaggca
     5101 caagatgccg agccaagaaa acaaaacagt ctcataaaaa actttttatt tcctttataa
     5161 tctgcaatgc cactaagcag tggtgaaagg aaggcgatta ctaaaaaaga aaaagataaa
     5221 gcataagaat aaagtgccgt gttggtgaaa gtgcgtccga gaaatgttac ctcatcacta
     5281 ccgttggcac tggtaatgct gttgtaataa atcggaaaaa tggctgtggt aatacttagt
     5341 gaataaactg aattggccca gtcataaaat gtccaggcgc gtaaaacttt aggatcgttt
     5401 tttttcatta aaatcaatgt tgatttattg ccatataaaa cttaatgtgc cattggtttg
     5461 acttccgcta aacgattgct gaacagcgta tggaagttga ttggtcatca acggagtaag
     5521 gtttttttca atcactacgg tttccattaa atcgtcagtt tcaggactga ggttatagtt
     5581 gaacacttta ataataattg acttgtcgaa ctgtgtaatg gcaaagggtg taataaaatt
     5641 gaagttgatt gctgtgccct gcggtgcatt attgctgact gttgtactga cataggtatt
     5701 tccattccaa atctggcagt aaatatcggc tgcagaacca tcgctatccc aagctgttcc
     5761 atctgattta tgaacaggcc agctgtttag actaaagtta ataatattgg cttttgtata
     5821 gggtgatgcg ttgatacctt ttattgttgt agctgttttg cctgctgcag tttcgccttt
     5881 taagacaata ttgaaaaatc catagtcaaa aaggtgcata acactatcgc ctgtaacttc
     5941 tgtactgcca tcggcaaaat tccatttata gttacatgga atagttgatt cagctttaaa
     6001 aataaaagtg tcgggatgtg ttttaaagtc attataaact gtgataccta aaaatggtga
     6061 cggactatca tcagattttt ttttgcaacc aaagtttaaa aaactgacta ctaacaatgg
     6121 tgataaaatt tgtgaaagta ctaatagcca atttggtttt ttagttcctg tactgcggca
     6181 atgccataac cgaaattcca ttattttttt aaaagaagtt ttttccattc tttaggaagg
     6241 ttttcactaa cagaaagacg ataattgtaa caaaccaaaa ccgtaagtcc atctgccaga
     6301 ataattttat tattatcagt tgttttaaaa acttcaaagc caaggtcaaa actttttgtc
     6361 cctacacgag tacaccaagt tctgacggaa atattatcat tcagcaatgc aggatgtctg
     6421 tagttgattt gtgtctgtgc cagaataaat cctgtttcgc gccagttgat gtttaatggc
     6481 agacgttcgc taaaatcaat tcttgctttt tcaatatagc ttaaataaac ggcattgtta
     6541 acatgcccca tccagtctat gtctgaaaag cggatttgaa tgggggtaat tatttcaggc
     6601 atatttactc tatgatgata cgttttgaaa tcaattgctt atcggtaatg atattgagca
     6661 aatacattcc tttggaaaca cctgttgtat ttaaagttgt gtaatcatgc aacaaaggtg
     6721 ttgaaggaat aatatttctg ccctgaacat caaacatgct gcaagaaaat gtttttccgg
     6781 gtatgttgat gttgatttgc ccttttgctg gattaggata aacatgaacg gcaggcaaat
     6841 caacagtatt tgtgcccgat atattttcgc aaagaatatc acttaaaaaa cgtgcaacaa
     6901 gtgtatcaat cagtacatca tcatgaatgc catgtacgtt accattgtag agtaatgcct
     6961 gataatgctg acttgaataa ccaaggttat tcattctgtc atttatcaca caggaaccaa
     7021 aaagatgtgg gtaattttgt gatacattca taggttgatt ccaaagcccc ggcaaatagc
     7081 cgcaaccaac agtgccatca cctgtttgat ggtatgagaa aagtgcaggg tctgtgttag
     7141 cttcaataaa agatgtatct aacaatgcac caaagagatt tactgctgca agcacgttgc
     7201 tgctttgtcc attaagattt aatcttccga aaatagaacc caaatcactc ctttgtacat
     7261 taccaaagag cgttgttaca tcgctaattg ctccgccatt tatcggagcc tcgctttgtt
     7321 tgtcaacaaa ggtaaaatgc atagctgtga tagagccagc actggcaccg ccaacaaaag
     7381 caagattaat attgcttgag tcaattgctg cacgtgcttt aagaaaacgc atggctccat
     7441 gcgcatcttg tacagcacga tatgctgctc taattacttc gcttgagtcg aatgtaaatg
     7501 gtgcaccaaa aaacaggttt ccataaaatc ctagtctgta tgacatggtt gcagaaacat
     7561 aacctctttc tgcataagct tgtgcgatga catccatatc atttttatta cctgtaaaaa
     7621 aacctccacc atgaatccat aaaattatag ggcgtgatgt gttgttgtcg cctattggct
     7681 tccagatatt catttgaaga ttcgccactg tattgttata ggcagtgtcg ctaccataac
     7741 ttatatctct ttcaacatag gtttgataga ttctcggata ataagggtca agacattgag
     7801 caaaactttg tccgctgcaa ataaaagcaa atacaaaaag taaaattcta ttcatcataa
     7861 aaaggcttat aaaatctgtt gactaaagta aggttaatag atagcatttg caagggtggc
     7921 tttttacaga cattaacatt aagcaaaata aagatccact tcaatttcaa ttctgcatac
     7981 tgcattgttt tcagcaacat tatttcataa gcaaattaca aaaaaggcca tctcaaaaat
     8041 gttttttgag acagcccttc tgaaccaaac tatcaaatat tttacttctt caccaactga
     8101 atttctgcag caatctgcac ttcttcgcta accaatacac caccggtttc taatgctgtg
     8161 ttccagttca gaccccaatc tttgcggtta atttttccgg ataatgtaaa tcctgccttt
     8221 tcctgacccc atgggtcttt catcatacca ccaaactcta cgttcaattt tacaggctta
     8281 gaaacacctt tcattgtaag ttgaccattc agtgcataaa tgtcgtcacc ggtttttgaa
     8341 aaactctctg acttaaatga aatagaagga tgattttctg catcaaaaaa gtcaccgctc
     8401 tttaagtgtg tgtcacgttg tgtgtcaccg gtagtaatgc ttgaagcatc catactgaaa
     8461 ttgatgtttg cattgctaaa attattgtcg tcagattgta cagtgccttc aaactttgca
     8521 aagtttcctt ttacattcga tatcatcaga tgttttactt tgaatgtaat ttcggagtgt
     8581 gcctggtcta tgccccatgt tgtttttgtg agtgttgtca taatttttat ttttttaaat
     8641 tgttaattgt tttgtttaat tgagttgcat tggaatatcc agcagtaata tttcggcacc
     8701 ttcggtttta gcgataaagt ttatttcaga ggtattccat aagccaatag catcacgctc
     8761 atacaacaca ttgttgttga tttcaaattc gcctttcact accatggcat aaacaccgtt
     8821 accttctttt tttattttat atgaggtgtt tttatctttt tcgaagttgc ccatactgag
     8881 ccatgcctgt tggtgtatcc acaatccgct tttttctgta tcgggcgaaa cgattgtctg
     8941 cagtttgttt tttctgtcac tgatgttgag tgaaagttga tcatagcgtg gatttacatt
     9001 ttctttgtcg ggataaagcc atatctgaaa taactttact gcctcagttt tgctggcatt
     9061 aaactcactg tgtgtcacgc ctgagcctgc actcatcacc tgcacatcgc catattttat
     9121 tatgccatga ttattcatgt tatccttatg ttcgagtgct cctgctaaag gaatagtaat
     9181 aatttccatg ttgtcgtgcg gatgtgtgcc aaagcccatt cccggttcaa catgatcgtc
     9241 attgagtacg cgcaacacac caaagtgcat ttgattggga ttatagtagt ttgcaaaact
     9301 gaatgagtgt tttgctttta accaaccatg atcggcattt cctctgtttt ctgctctgtg
     9361 aattacttta ttttccatat ccttaaaatt tatggtacaa aagtagaacg gccttgagcg
     9421 gtcagcaata acctgaatta ataaagtgac ttaatttagg ttaagttaat ttgtgtgtgt
     9481 gatttttgtt gtcagctttt agtcatacgt gcacgcatct tactgaaaaa ttctggcgtg
     9541 acaccaatat aggaagcaat atatttctgt ggtaaacagg aaaccaaagc aggatacttc
     9601 agacagaatt tttcgaatct ttcttccgcg gtcagagaaa gtttatcgag aatccgctcc
     9661 tgctgacttg ccatactgtt ttcaatcaat attctgaaaa agcgttcaaa cttggggatt
     9721 ttatcaaaca agatatctct gttttctttt gtaagcacaa caatttcact ttcatcaatg
     9781 gcatcaataa atagaattcc cggccggcct gttatgctgc tataaaaatc gccaatccac
     9841 catcccggtg gagcaaattg taaaatatgt tctgttccgt ttttatcaat attataagca
     9901 cgcaagcaac ctgaatttac aaaacatgat gccgaaaaaa cttcgcctgc ctgcataata
     9961 aattgcttgc gtctgactaa acgtgtactt aataacgatg taaatattgt ttcttcttct
    10021 tctgaaagta aaatatatct tgaaatattt ttcagaataa gattaaaagg tttgttgcta
    10081 tctgtttttg ccatgttgtt caatataatc aatcagggca tgaataattt tgatatgaat
    10141 ttcctgtgtt ctgtcggcat atccggaatg aggaacgcga atttcaacat cgcaatgatt
    10201 ttttaatgca ccaccatctt tacctgtcaa tgcaattacg gtaatatttt ttgattgtgc
    10261 cactttagct gcttccaaaa cattggcaga gttgccactt gtgcttatgg ctaataaaac
    10321 atcgccttta tttccaacac cctcaataaa tcttgaaaaa acataattga aaccgtaatc
    10381 atttccaaca caactcatat atgaaggatc agaaatgctg attgctgcaa ttggttttct
    10441 gtcgttacga taacgtcctg taagttcttc ggcaaagtgc atggcatcac acatgcttcc
    10501 accattgccg cacgatataa ttttgcctcc tgctgcaaga gaggcagtca tcaactcacc
    10561 ggcttttgtt acggctgcaa tatttttttc gtctgataaa aaagtttcaa gtactttttt
    10621 ggcttcttca aaatgctgac gaattatttc tgacatatat aagtttatat agcacaaaga
    10681 tataagatat tttaagttta ttttgtttgt aatatatcat aacaggctaa taatgttgct
    10741 aaacatttat tgtcgtctaa gttgttattg atgaatgaaa aagtgtttgt gagggaattt
    10801 gttttttatt tgcattaatt ctttttttat gcaggttaag aacaaattta tattagcact
    10861 tctttcagca atactgcttt cgttaagctg gcctgagcgt ggactaaccc cacttgtttt
    10921 tattgcatgg gtaccattgc tgcttttgga agcgtatagc tcgcagaata aggaacagaa
    10981 aaaatttagg ttgtttggtt gggcatattt tgccattctt gtgtggaatg cattaaccac
    11041 atggtgggta tgcaatgcca gttttggtgg cggcctgttt gctattttct gcaatgcatt
    11101 gtttatgaca ggtgtgtttt atgcttttca tcttgttaaa aagaaaaagg gacatgtggt
    11161 cggttatgca gcattaataa ttttttgggt cgcttttgaa tacctgcatt taaactggga
    11221 gttaagctgg ccctggttga cattaggaaa tgtgtttgca acaaaaccac aatggattca
    11281 gtggtatgaa ttgacaggag tgttaggtgg cacagtttgg gttctgttgg tgaatatttt
    11341 aattacaaga atttttcttg catcagcaga taaattgaag cgcaacatta tgtatgcaac
    11401 tattgcaatt atttttcctg tgctgatttc aatactgcgt tattacactt ataaagaaga
    11461 agagaatcct gttaatgtta ttgcaacaca acccaacatt gacccttatg gagagaaatt
    11521 ttccggaatg agtaatgttg accagttgat tcaggtgtta cgcttagcca atctcgaact
    11581 ttacgattca tccgatgtta ttcttgcacc tgaaacagcc attgccaatt caatctggga
    11641 agatgagata gaactttatc ctgaaataaa attaatcaga aattacttgc aggcaaatcc
    11701 cggtgcaact atcattattg gtgcatcaac aaaccttgct tacttgaatg gacaaaagcc
    11761 atctgcaaca gcaagaaagt ttaaagatca ggatggttat tatgattcct ataacacagc
    11821 tttgctcttt gaaaatggaa agcctgcaca gatttttcat aaatcgaagt tagtgccggg
    11881 cgttgagaaa atgccttatc caaagatttt cggttttctt gaaaaacttt caatagacat
    11941 gggaggcact gcaggaagtt tagggattca ggatgagcca acagtgttta caacaaaaaa
    12001 cagaatgatt gttgcacctg ttatttgtta tgaatcaatt tatggaggtt atcttgcaaa
    12061 gtatattcgt aagggtgcac aatggattgc aattattact aatgatggat ggtggggcga
    12121 cactccgggc tatcgtcagc attttaacta tgcacgattg caggcggttt ctttacgaag
    12181 aagtataggc cgttcggcaa atacaggaat cagtggtttt atcaatcaac gtggtgatgc
    12241 atttcaacaa acaccctatt ggaaagatgc agtagtgcgg caggctatca acaagaacga
    12301 taaattaaca ttttttgcac aacatggcga ttatcccggg ctaatagctt caatattggt
    12361 gttgccggtt attgttatta cgtttttcgg aaaaagaaaa aatcagtagt tttttttcgg
    12421 aattttgtgc aataaatttt aatagtaatg gaaaattttg atgtggcagt aattggctcc
    12481 ggtcccggtg gctatgtagc tgcaattcgt tgtgcgcaac ttggctttaa aactgtaatc
    12541 atagaaaagt ataatacact tggcggcaca tgccttaatg ttggctgtat tccttcaaaa
    12601 gcattgctcg attcttctga acattatttt aatgcggtaa ataaattcgg tgagcatggt
    12661 ataaaagtca aagatattaa agctgatatg gagcagatga ttaagcgcaa ggctcaggtt
    12721 gtgaaacaaa cttgcgatgg cattagcttt ctgatgaaga aaaataaaat tacagtaatg
    12781 acaggtcatg ctacttttgt tgatgcaaaa caattggatg ttgttgatgc cagtggtaag
    12841 tcaaacagaa taggtgcaac taattttata attgcaacag gatcaaaacc atcatcactg
    12901 cccggaattg aaattgacaa gaagcatatc atcacatcaa cagaggcatt agagctgaaa
    12961 actattccca atcatttggt ggttattggc ggtggggtta ttggtcttga gttgggatcg
    13021 gtttatgcac gattgggagc aaaggttact gttattgaat atatgaatgc tattattcca
    13081 acaatggatc gtgcattggg aaaggaattg cagaaagtgt tgagtaagga agggtttacc
    13141 ttcttacttg gtcataaagt gcaatctgta aaatcatcag ccaaacaagt tgaagtggtt
    13201 gcagaaagca gcaatggcga aaaggttaac attaccggag attattgttt ggtatctgtt
    13261 ggccggaagc catacacaga aaatttgggt cttgataaag caggcgttgc aattgatgaa
    13321 agaggaagaa ttattacaga tgaaaaatta caaaccaacg tacctggcat ttacgccatt
    13381 ggcgatgtaa ttaaaggggc aatgcttgca cataaagccg aagaagaagg cgtgtttgtg
    13441 gcagaacagc tggcaggtca gcagccacat atcaattaca gacttattcc cggagtggtt
    13501 tacacatggc ctgaggttgc atccgttggt tatactgaag aagaacttaa agaatcagga
    13561 acaaaatata aaacaggaaa cttcccattc aaagccagtg gcagagccag agcaagcatg
    13621 gataccgatg gttttgtgaa ggtgttggca gatagtgata ctgatgaaat tcttggtgta
    13681 catattattg gtcctcgtgc ggcagatatg attgcagaag ctgtggtagc aatggaatat
    13741 cgtgcatcag cagaagatat agcaagaatt tgtcatgcac acccaacatt tacagaaagt
    13801 tttaaagaag cctgtcttgc agccactgca aacagagcca tacacatctg attaaacaca
    13861 aatcccctaa cacataattt atgaatattc agataaaaga aattgtcacc gtttcaatga
    13921 ttttgttttc ggtgatagac atcattggct ctgtaccggt aataataggt atcagaaaaa
    13981 gtgtaggaag cattcacccc gaaaaaacat cgttggtggc attgatgatt atgatagttt
    14041 ttctctttgt aggagaaaat attctttcat tgattgggct tgatgtttca tcatttgcga
    14101 ttgcaggttc attcattctt ttttttcttg ctctggaaat gattcttggt gtaaagcttt
    14161 ataaagatga aatgccggca tcggcaagta ttgttccgat tgcatttcca ctcattgcag
    14221 gtagtggcac catgactaca ttactatctt tacgcagtca gtatgatatg ctaactatca
    14281 ttattggcgt gttgctaaat atgatttggg tatatctctg tctgaaaaat cttggattta
    14341 ttgaaagact acttggtcca acaggtatag ccatcgtcag aaaagttttc ggttttattt
    14401 tattagctat tgctgtaaaa cttttcagaa gtaatctcgg aatttaaact catggctaaa
    14461 aaaaaattct atgtagtttg gaaaggagta atgcctggtg tatatcactc atgggatgat
    14521 tgtaagaggc agatagatgg ttatgcagga gcattgtata aaagttttga atcagaaaat
    14581 gctgctctta cagcattcca aaagggattg tcaggttttc agaaaaaagg tgaagctcat
    14641 cctttgattc atgtagaaaa aacagcatca aaaccaataa aagaaagtat tgcagttgat
    14701 ggtgcatgga atactaaaac gttggaaagt gagtacagag gtgtagaggt aaaaaccgga
    14761 aaacaacttt ttcttaaagg tccttatgct gatggtacta acaacatcat ggagttttta
    14821 gctcttgttc atgcattggc ttattgtaaa cgtcatcaga ttaatttacc catttattcc
    14881 gatagcatga cagctttgaa atgggtaagg acgaaaaaag caaatactaa attggcttca
    14941 acgggtcgaa atgacgaatt gtttgaatta attgtccgtg cagaagagtg gttgaagaat
    15001 aattcttatg ctaacaaact gcttaagtgg gaaacgcaaa tttggggtga aaatcctgca
    15061 gatttcgggc gcaagtaatt gaaaattgat actttttatt ttttttcgtt taaattttta
    15121 cagtcagaca ttgttttttt aaaatttgaa aaagcttgaa tagctctcgg gggtattcaa
    15181 aaacaatatg ttgtaagtaa aaactgattt atgtcatatc aatacttaat tgctatcata
    15241 aaatagctat tttaggcgga gtagatttgc aatcgttttt tatgagtgta ctttttatac
    15301 taattggagc tagcttggtg gtggcaacag gtttcttggt ttcttttttt tgggccgttc
    15361 gtagtgggca gtatgatgat gattactcgc cttctgtgag aatacttttt gacaatgaca
    15421 aaactttaac atcagataaa tcaaattcaa acataacaga taactaaaat tatgacaatc
    15481 gaaaagtttc gctatgacaa caagattgtt gccatgtttg catgggcaac tataatatgg
    15541 ggtatcgtgg gcatgcttgt cggcctgcta gccgcttttc aacttccttt tcctgtatta
    15601 aattttgatg cgcatacaac tttcggacgt atcagaccat tacatactaa tgctatcatt
    15661 tttgcttttg tgggtaatgg aatgtttatg ggaatttatt attcactgca acgtttgctc
    15721 aaagctagaa tgtacagtga tttattaagc tatattcatt tttggggttg gcaattaatt
    15781 attgtaggtg cagcaatcac tcttccttta ggaattactg ccagcaagga atatgcagag
    15841 ttagaatggc cacttgatat tgctattgca attgtttggg tgatatttgg tatcaacatg
    15901 tttggcacca tgatgcgcag acgtgagcgt catctttatg tggcaatttg gttttatgtt
    15961 gctactgtag taacagttgc tgtattgcac attgtaaact ctcttgctat acctgtaaca
    16021 ttatttaaaa gctattctct ttatgcaggt gtacaggatg cactcgttca atggtggtac
    16081 ggtcacaatg cagttgcatt ttttcttaca acaccctatc tgggattgat gtattacttt
    16141 ctgccaaaag ctgccaaccg tcctgtatat tcctatcgtc tttcaatcat tcacttttgg
    16201 tcattaatat ttctttatat atgggcaggg ccacaccact tgctctatac tgcattgcct
    16261 gattgggcac agtcattagg tgttgtgttt tcaataatgt tacttgctcc atcatggggt
    16321 ggtatgataa acggattgct gacgctacgt ggtgcttggg atagagtacg cgataatccg
    16381 gagctcaaat tttatgttgt tgcagtaact gcttatggta tggcaacttt tgaaggtcct
    16441 atgctttcac taaaatcggt taatgccatc agccacttta cggattggac aattgctcac
    16501 gtacacattg gtgcattagg ttggaacgga tttctgactt ttggaatttt atattggctg
    16561 atcccaaaat tgttcaacac aaatttatat tctaaaaaat tagccggaat acatttttgg
    16621 ctcggaacac ttggtattgt tttttatgca attccacttt actgggcagg cattacacag
    16681 catttcatgt ggaaagaatt tactgaggat ggtgtgctgc gttatcccaa tttcctcgaa
    16741 acagtagttc aggttattcc aatgtataaa ttgcgtgcat tgggtggatc actttatttg
    16801 cttggctcat tggtgatggt ttacaatttg attaaaacag tgcgtgcagg aaaaatggtt
    16861 gctaatgaag aagcagaagc accggcatta gaaaaaaatt atgttgctca tgatgaccac
    16921 tggcatcgct ggattgagcg cagacctgtt caaatgctta ttggaagtct tattgtaatt
    16981 ctcataggag gaatggttga gattattcca acttttatgg tgaagtcaaa cgtgccaacc
    17041 atagcaagtg ttaaacctta tacacctctt gaattgcagg gtcgtgatat ttatgtacgg
    17101 gaaggatgtt acacatgtca ctcacaaatg gtgcgacctt tccgttatga aactgaacgc
    17161 tatggtgaat attccaagcc tggtgaattt gtttatgacc atccgtttca atggggaagt
    17221 aagcgaaccg gccctgacct gcatcgcgag ggcggaaaat atcctgactc atggcactat
    17281 aatcacatgc tcgacccgca aactatgtca cccggaagta ttatgccttc gtatgcattt
    17341 atgattgatg atgatttaga cactagcact actgcagcaa aaatacgtgc tatgcaaaag
    17401 ttaggcgtgc catatcctga tggttatgat gcacatgcca acaaagatct gatgaagcag
    17461 gcaaacagta ttgcatcaaa tcttgagaaa gatggaatca aagtaatggg taataaagag
    17521 attgtagcca taattgcata cttgcaacga ttgggagttg atataaaagc agaaaagact
    17581 actgcatcag ttaattaaac aggaggaata ttatgtataa agaagtaatg tcaaccataa
    17641 aagatatcag tatctatcct gtattttctt tcgtcatttt ctttgttttt tttaccattg
    17701 taggtatttg ggttttgaaa tcaaagaaag aagattttga gtctgtaagt aaactgccat
    17761 tagaagaaaa aaatgattaa aactttaagt aatgaaaagg ataattaatt taatatgttt
    17821 gataatgctt atctcattat ctgcatgggc cggagataat tcacaggcaa cagaaacagc
    17881 aaaagtagca gccgctcaac ctccggactg ggcaatgaat gcaaatactt atcttattgc
    17941 atttattgtg attgttttac tcgttactat attggcatta ttcagaagta gtctgaagct
    18001 gattaaaaaa ataacatcgt tttgtattgt tatgtttatt tcgggttctg cttttgccgc
    18061 agaaactact gaatcaaaag aagtagcaaa ggtaactgca gcacaaccac ctgactgggc
    18121 attacatcca aatacatatc tcattgcatt catagttctt attttacttg ctacaattat
    18181 tgtattgtac aaaacaaata tgaaacttat caaaatgatt gcacctcatg catttgacga
    18241 tgagaagcaa acagcagtag aaggagcagt ttccggagtg aaaaaccgtg aaacatttat
    18301 gcgtaaggtg tatttgcgtt tagtggatag tgttcctgtt gctaaagaac aggatattct
    18361 tttagatcat gattatgacg gtattcagga gcttgacaac aatctgccac catggtggaa
    18421 gtggggattt tatattacaa ttattgctgc tgtggtttat atgctggctt atcatgtcac
    18481 aggaacagga aaattacagg ccgaagaata tgctgatgaa cttgccgtag cagcaaagca
    18541 gaaagaagaa cgaatgaaaa attcagcaga gaatattaca gaagaaaatg tgacagtgtt
    18601 ggttgatgct gctgaaattg caaaagggaa agatatttat gaaaaaaact gttctgcttg
    18661 tcacatggct gatggtggtg gaatggtagg gccaaacctt accgataact attggctgca
    18721 tggtggtggt gtaaagaata tttttaaaac aattacttat ggtgttcctg ctaaaggaat
    18781 gattagttgg caggcacagc tatcaccaaa acaaattcag gaagtaactt cttttatcat
    18841 gactcttcat ggaacaaaac cggcagcgcc taaagagcca caaggtgatg agtggaaaga
    18901 agatgctact gccactgcag gagataccac tgccgtagct tcggcagaag ctgcagttaa
    18961 ataaaattaa atctcttttt tatggtggag aataaaatgg agcatattga tgaatcgttc
    19021 agagatagta ttgcaacagt tactgaagat ggcaaacgtg catggattta tcccaagaaa
    19081 cccaaaggca gatattacaa tgcccgtact tgggtaagtg ttgtatttct gattatattc
    19141 tttgcattac cattaattaa gattaatgga catccgtttt tcctgctcaa tgttgtagag
    19201 cttaaattcg tattgtttgg aattgctttt tttccacaag acttttattt gtttgtattg
    19261 gccatgctaa cctttgtagt gtttattgca ttatttacag cactgtttgg aaggctttgg
    19321 tgtgggtgga cttgtccgca aactgttttt atggaaatgg tatttcgcaa aatcgagtat
    19381 ttgattgaag gcgattttat gaaacaaaaa gcactcgata agcgaccttg ggatgcagaa
    19441 aaaatcagaa tcaagactac aaaacatctt gtattttttt tcatagcgtt tcttatctct
    19501 aatacttttc tgatgtatat aatcggattt gatgagtgga aaaaaattat tacagataat
    19561 ccattaaacc atattggagg attgtttgca accatcattt ttgcaggtgt gttttatggt
    19621 gtttatgcac gttttcgcga gcaggtttgt attgtggttt gtccttatgg ccgcttacag
    19681 ggtgtaatgc tcgaccctaa tactgtggtt attgcttatg actataaaag aggtgaacca
    19741 agagggaaac ttagtaaaga caataatgag caaggcgatt gtatagactg caatcaatgt
    19801 gttcaggttt gtcctacggg cattgatatt cgaaatggaa cacaacttga atgtgtaaat
    19861 tgcacagctt gtatggatgc ctgtgatgac attatggata aaatccagaa gccacgtgga
    19921 ctcatcagat atacaagtga gaatgccatt aagaaaaatg ctaaatttaa aattaattcg
    19981 cgcattattg gttattgcat agtcttgacg atattgtttt ctacgttcgt agttttatta
    20041 gcaagacgta gtaatattga aacaacaatt ttacgtgcct cagggctgtt gtatcaaaaa
    20101 cctgatgaaa gccatatcag taacctttat aacattgctt tgataaacaa aacttttggt
    20161 gatatgccta ttgaattaaa agttgttgaa ccaaagggag aaataaaatg ggtaggcaat
    20221 ggcattacac accttaaaga gcaatcagtg gcacagagta ctttttttct tatattaccc
    20281 aagacggaaa ttaaaaaaac aaaaacaaaa gtgatttttg aaatctggtc agaaggagaa
    20341 aaaatagatc gttatgaaac gtcattctta ggccccaata attaatttaa aaatgaaatt
    20401 caactgggga cattgtattg cagtagtttt agtgattttt gcatcatcaa tattggtgtt
    20461 tgtttatgtt gcatctaagc agaagaatga attagtgact gaaaaatact acgagaaagc
    20521 agtagcttat cagcaaagaa ttgatgcaga aaaaaatgca ttaaatgaaa acgcagaggt
    20581 taaatacaat atcaaatccg gtcagctaat gttggtgtta ggccgaaata tgccaaatgc
    20641 aaatggtact tttcagtttt ataagcctga taatgcagct aaagatttta atacttcatt
    20701 tcaggttaac gataccgggt tttatagtgc tgttattcct caattaacgc atggccggtg
    20761 gagcattaac gtaagctgga atgttgacag taaagcgtat ttttacgaaa agaaaatttt
    20821 tattccctga tgaacgaaaa cactttctat tacggagctt tgctacttgg ttttcttggt
    20881 agctttcact gtgtaggaat gtgtggtcca atcgcactaa tgcttccggc aaataagtta
    20941 aataatcgca gtgtggcatt ttttggcaga atgttttaca acattggcag gatgataacc
    21001 tatatgttta ttggaatgtt gtttggatta ttgggtgcag cttttgtttt aaatggattt
    21061 caaaaacaac tgagtattat aacaggagtt attttgttat taattgcaat atttacttta
    21121 attccttcta ccagaaagaa agtacttagt gtttcatcaa aaataaatat tcctttcaga
    21181 ggagtattaa aaaaactttt ttcaggaaaa acagccttac atcattttgg tattggtatc
    21241 attaatgggt ttcttccatg tggatttgtt tatgtagcaa cagcaggagc catcaatgca
    21301 ggtaatattt tgaatagtgt tctgtatatg gcattcttcg gattaggtac ttttcctatt
    21361 atgcttcttg tttcctatgc agcatcgtgg tgtaattttt caatgtcacg ctatgtaact
    21421 aaagccacgc catggatatc tgcagcatta gcgctatttc ttatctacag aggagctttc
    21481 atgaatttcg atgccgattg tcataaacat tcgcatccgc caacaatgat tgaatgtagt
    21541 aaacccgaaa cgctgtaaat tttgttttag gagattgttc aatagccgat attcggtagc
    21601 ttaatgatat ttgtatattc gcaacgctac attaaatcgg aaaggtaaat gagtcataaa
    21661 ttattagatg ccttgatgca cctttttgcc attctggcaa acgctgaaaa attttctctc
    21721 gaaggtagaa gtatagttga gtctttttta cgtcagcaaa tcagtcgtgc ttatgtgtta
    21781 aaatatcttg aattgtttga cgcatatctt gaaaatatta gaggcaaggc aacagaagga
    21841 aagttgaaaa agcgactttc agtgaactct gtaaaagtat tgcgcatctg taccgacatc
    21901 aatgccgaac ttgataccag acaaaagcat attgttttaa tgcggctgat tgaattcgtt
    21961 tatacttctg aaaaaattat tgacagccag gaaagagaat ttctttatac agttgctgag
    22021 gtttttcata tttcgcctga tgatgttcag ttgtgcatgg aattggcatg taataataca
    22081 gaagataaat attcagatac tcctaaatat ttagtattat cttcacaaga tgatgagaac
    22141 aatactttaa atactaaaac acaaagtata tcaggactaa gtggtttttt ccgtttttta
    22201 tttcttaaaa atgcaggatt actttttgta cgatatcacg gtacagagtc ttttacacta
    22261 aatggattac cactaaatga cgatgtgtgt tatgtttttt ctgcaggttc agttataaga
    22321 ggttcaaagg taaatacaat ctattattct gacattatca gactgttttc aaattttaag
    22381 caacagagtt tgttgcattt tgatgtgcaa catgtgtact atgattttgc aaatggaaaa
    22441 cgtggcctta atgatgttac ctttagtgca acatccggaa atctagttgg cattatgggt
    22501 ggaagtggcg caggaaaatc cacgctacta aatatcttaa atggaaacaa taagcctacg
    22561 caaggaaaag ttactattaa tggaatagat ctgtatcggg agtctcatct gattgaaggt
    22621 atgataggct atgtgccaca agatgatttg ctgatagaag atttgtctgt ctatcagaac
    22681 ttattttata atgcaaagct ttgctttggt aatttaaccg atggtgaaat tgatgaacga
    22741 gtaaaagcac ttttacataa tgttggtctt ggtgaagcaa gccatctgcg tgttggaaat
    22801 gcactcagta cttctatcag tggaggtcag cgcaagcgac tcaacattgc attggaatta
    22861 atcagagcac cggaagtgct ttttgttgac gaaccaacct ccggtttatc ctctctcgat
    22921 tcagagaatg ttatggatat gctcaaacag cttgctaatc acggcaagct ggtttttgta
    22981 gtaatacatc agccatcgag tgatattttt aaaatgttcg acaaactttt tgtacttgat
    23041 attggtggct ttcctgcata cttcggcaac cctgttgatt cattattata ttttaaacag
    23101 cgggcttctt ttgctgatgc cgattatagt gaatgtggaa cttgtgggaa tataaatccg
    23161 gagcaggttt tttctattct tgaatcgaaa gtgatggatg agtttggaaa tccaacacag
    23221 caacgtaaac tgactccttc agattggtat cagttgttta aggaacatag cagtggttat
    23281 ggtaaagtga atgagcgaca acctgtacag ggaagtgcaa atcagaaacc cggttggatt
    23341 caacagctga aagtatatat cactcgtgat gttctggcca agcttaaaaa caaacagtac
    23401 atgctcatta catttcttga agcacccgtg ctggcagtta ttcttgcctg gtttctaaga
    23461 tattttcatg caggaggtag ttatattttt agtgaaaata aaaatcttgt agcctatctt
    23521 tttatggcag ttattgttgc tattttcatg ggacttactg taagtgccga agaaataatc
    23581 cgagatagaa agatgcttaa acgcgaacaa tttcttaacc tcagcagaag cagttacttg
    23641 tcagcaaaag tattggttca gtttttaata tcggccattc agactttttc atttgtcatt
    23701 ataggaaatt tcatttttgg tattcaaagt atgagtttag attattggct aatgctattc
    23761 tctgtttcct gttttgccaa tttgctggga ctaaatgttt catccatgtt tgacagtgct
    23821 gttactattt atatcctcat tccttttctg attataccac aaattatttt aagtggcgta
    23881 atggtaaagt ttgaagacct gaatccatat ctaacttcgc agcgacatgt tcccgtaatt
    23941 ggtgagatga tggtttcgcg atgggcattt gaagcgcttg cagtgaatca gtttagtaat
    24001 aatccttatc aaaaaaactt ctttaatgtg gacaggcaaa tgagcgaagc tacttacaag
    24061 aaggatttct ggttgcagaa gttgaatgat attgctgatg acatttctaa aaataaatct
    24121 aaaaatccaa aacaagatgt tgcgttaatt gcaaatgaat tccataaaga ggctttaatc
    24181 aacaaaaatt tcaaaccttc agttgatttt gagaagtcag atgctttaga aattgcatca
    24241 caaattaaac cactgacaga cacacttcgt aatttctata ttgaaatgta taacaatgca
    24301 tctgctgcaa aggatagact tgtcaagaaa gcactgagtc attcaactgt agcgcgaatg
    24361 aacaaacaaa aagaaaagtt taccaataat gccttaaatg atcttgtttt aaacagaaat
    24421 gattttacca ttcttgtgca agatggcaac agcattgtgc aaagatttca acctgtttat
    24481 atgcagccta acccatcagc attattcaga gcaccactgt atgtttatga gaagaatttt
    24541 ttaggaatga aaattcatac atggtatgta aatcttttta tgatttggct tatgagcttt
    24601 attttgatgg ttacacttta ttataacagc ttcagaaaat ttatcggttt gatgggacaa
    24661 ggtagaaagt gatagtaagt tttttatcag acctcaataa aaaactgcta ttaaatcatt
    24721 taatagaaaa atgatttaat agcagttaag tttaaattgc tatcttatta aaaaagcatt
    24781 agccgcactt actgtggcca cagcttttgc atttaataca accctcttca aaatacaatc
    24841 cttccttgtc tccacactct gcacaaactt tatcgttgac ttttgtaccg tcaggaataa
    24901 attgtttcaa tgcacgcatt acaccatttt tccaggtgtt gatgctatca gaataaagat
    24961 gtaagttttc taccagatta actacttgtg gtattggcat tccatgtcgc aatacaccac
    25021 tgataagttt tgcatagttc cagaattctt tgtcaaacga acgcgatagc ccttcaatag
    25081 ttactttaaa tccttcttta tcattaaact gaaaatcgta tcttgattta ccatgtccgt
    25141 ttttactctt gataatccat ccagaagaaa cccatgcagg aatactgaat gcatcttctg
    25201 ccttaccggt aaaaatttca taaggtttgt tgtctaatat gccgatgtat gcaatccatt
    25261 tttcatctgc attcataaaa cggataactt ttgcagcaag tatttcagga cgctttggtg
    25321 cactcgattc tgttattata ggtttagctt ttttctcttc tttttttacc aatacaccac
    25381 ttcgtgaacc ttctctgtaa acagtaatgc cttttaatcc ggcactccat gcctgcatat
    25441 aaatttcgcc aacagtttct gccgaaacat tttcaggaag attaatagta gagctgatag
    25501 agtgtgtagt atatttctgc actactgcct gcatttttat tctttttttc cagtcaattt
    25561 cattagcagt acttcctgca taagggcttt tactgtagtc tgtactgttg tttgcagtca
    25621 tccatttttt taatccgtga tgataaactt caaactcctg ccatgcatca ccggactgat
    25681 ctacaaaact tgttttagcc ttgctgtctt cggcattaac ttttcttctg cgtttataac
    25741 ttagcatgaa cactggctca ataccagatg atgtctgtga tagtaaactt aatgttcctg
    25801 ttggcgcaac agtactgata gaaatatttc ttcttccata tttcatcatt ctgttgtaaa
    25861 tgtcgggtaa ctcggctttc atcatattta cgaactccga ctgatcttca attgccgtgt
    25921 cgaagccttc aaacgttccg cgttcaatgg ccatatcaat acttgaatca aattctgcac
    25981 gacatttttc tttcatcatc atttctacaa tgctaagagc attgtcagag tcaaaagcaa
    26041 tattcatggc agcaagcgca tcggctaatg ctgtaaatcc aagtcctgta cgtcttcctt
    26101 ttttacctgc ttcatacaat gttatccaag tacgcatttc tacttcttta atgtagtcag
    26161 gttcaggatc cgattctatt ttttgtagaa ttctttcaat agcttccaat tccaaatcaa
    26221 ccaaaccatc catgagtcgc tgtgactcat aagtaacttc ataaaaacgg gtgaagttaa
    26281 acttagcctt ctgtgtaaat ggatgatcta caaaactcaa taaattaatt gcaattaatc
    26341 ggcaactgtc gccaccctgc attgcaattt cagagcaagg gtttgttgat acgtttttaa
    26401 atccaggata aacagaagag gtagaataat gatgttgtct gtcccagaaa ataattcccg
    26461 gttcagcagt acgatgcgca caggcaatga tggtcttcca taattcgcgg gcgttaactt
    26521 ttttctgtat ataaggatca gtagaatcaa cagggaatct caggttgaaa tcagtattct
    26581 cgttaactgc aaccataaac tcatctgata atttcacgga aatatttgca ccggtaactt
    26641 tactcaggtc ttgttttatg gtgataaatt tttctacatc agggtgagca atatctaatg
    26701 taagcatcag tgcacctctg cgtccaagtt gagcaacttc acgtgtggtg ttagaaaaac
    26761 gctccataaa agatacagca ccggttgtag tacctgctgc attggaaaca cgcatacctt
    26821 caggccttat agtagaaagg tctaagcctg caccgcatct gcgcttatat aattgcgcaa
    26881 gttgctggtc ggcaaaaaat attccaccgt atgaatcatg taattcggga agaacaatac
    26941 agttggataa agatgctatc atgaaggggt tgcctaatgc agacatgaca ctgccttgtg
    27001 gaataacata acggaagcga tcgaagagtt gtgtgatttt ttctgtcgtc agttgttggc
    27061 gtttttttcc ataatcagac agatgcgtag ttctgctgcc atcgttcaac agattgtatt
    27121 ttgcttcaac tctcgcaaat tcacgtgcca tacgcaaatg catatcatgt ggtgttcgct
    27181 ctacaaactt tccttctttg tttttcatgg catatttatt catccatgta gtagcagcaa
    27241 gttcatcgcc ttcaaaatat tccaaacttg ctttcaatac gctttcatat tcgtaggttg
    27301 tcatctgact ctcagttgta atggtttcaa tcaacatatt tagaatttct tatttgttta
    27361 agtcaagcaa taatatagac aaaaaaaatt ctttcataag gttttgacta atatttatca
    27421 gacgttgtta acaaccattg ttcactaatt ttattattaa caaagtggat caaacaaagc
    27481 gtttgaatta taagcgattg gtatatttgc taacacggag ccaaaaaaca aatgattata
    27541 atcattagca aaatttaatc aaatcattaa gcaatagaaa atctatttga ttctttgcac
    27601 tgtttaaatt ctaatatgaa attcaagaca attatcgagc ctttcagaat taaaatggta
    27661 gaacctatcc acctcagtac ggaggagcaa agaatcggtt atctgaagca ggcgcattac
    27721 aatccatttc ttcttaacag caatcaggta cttatagatt ttctgactga cagtggtact
    27781 tcggctatga gttccgacca atggtcggct atgatgaacg gtgatgaatc gtatgcaggc
    27841 gccagcagct ggctgcgttt tgaaaaagaa gtgcaaagcc ttacaggaat gccttttgtg
    27901 ttgccaacac atcagggtag ggctgcagaa agaattcttt atggcaaact tggaggtaag
    27961 ggaaaaacgt ttatcagcaa cacgcacttt gatactaccc gagcaaatat tgagtttagt
    28021 ggtgccgaag caatagatat tccggtgaag gagggacttg actttgcttc tgatttccct
    28081 tttaaaggaa atatggatat agcgttactc gaaaaaacta ttgcagaaaa aggtgcagca
    28141 aatataggtg cggtaatttt aaccgttacc aacaatagtg gtggaggtca acctgtgagc
    28201 atggagaatg ccaagtctgt tgcaaatgta tgtcgtaaaa acaaagtgtt gtttgttatt
    28261 gactgttgcc gtgttgcaga aaacagttat tatattcatc ataacgaagc aggatatggg
    28321 aataaaactt acaggcagat tgcagaagaa atgatgtgtt tgggcgatgc tgctgtgatg
    28381 agtgctaaga aagatgcatt agtaaatatg ggcggatttc ttgctgtgaa ggatgctgat
    28441 ctagcagaat cgtgtaaaca attgttgatt attactgaag gctttgccac ctatggcgga
    28501 cttgccggac gcgatatgga agctatagca gttggcttgc aggaagtgtt tgatccgggt
    28561 tatttgaatt atcgtattgt aagtacaagg tatttgggcg aaaatattaa agccaaaggc
    28621 gtgaatatta tttatcctgt tggaggacat gcagtttatg ttgatgcaaa aagtttatac
    28681 ccacacatac ctccgcatga atatcccgga caggctctgg tatgtgaatt gtaccgtatt
    28741 gggggtttac gcagtgttga aataggttct gtaatgtttg ggaaatatga ttcggatggt
    28801 aagctgattc ctgcacctat ggagttggta cgacttgctg ttccaagaag agtttataca
    28861 caaagccaca ttgactatgt gattgaaatt tttgatgaga tagtgcgaac aaaagataag
    28921 gttaaaggat taaagattgt aaaagaacca cagtttctgc gacattttac tgcacatttt
    28981 gagccgcttt aatttttatt tgtgattaac agttgagcat tacttattgc aaaaaagtat
    29041 tttgtaatat ttgaagttaa tagtagcgtt taccatccac cggtggcacc accacctccg
    29101 gtgctaccac caccaaagcc tccaaatcca cctccgctac tgccaccgcc ccaactgctg
    29161 ccaccgcctc cccaaactcc accacctccg gaagagttgc gtctgttatt ggctgcattc
    29221 aaaagcatcc aggcagtcca gaaacttaaa ttgttgagat gtgcatagcg cctgacagaa
    29281 aagactttca tcagcataat caaaccgaaa attaaaaaca caaaaagtat tggtgcccat
    29341 gccggtgaat tcttatttgc attttcgcca tctgaaacaa attctccttt tgttcgctgc
    29401 ataatttctg taactgcagc atcaagtccc tgataatatt gttgcgattt gaaggcgggt
    29461 tttagcttca gctcaataat tcttttacaa atggcatcag gcaatgcacc ttcaacacca
    29521 tagcctgtaa tgatggtcat gcgcctgtct tccatagcag ctaaaatcaa cacaccatta
    29581 tctttattct tctgaccaat accccaatct ctgaaaagtg taattgcata atcatccaca
    29641 ggatattcac cggtactacg aattaataca atggcaattt gtgtagaggt gctatcgtta
    29701 aattgtacaa gtttttgttc taaaagattc tcatcaacat cattcaagac cccggcatag
    29761 tcgttgacta aacggggtgg attgctttga ggtggaattt gttgtgcatt gccaaccatt
    29821 gcaacctgta gcaacaatag catccataat aaaattggac gtgcctgttt tttaatcacc
    29881 aaaaaccact tcattggaaa gttcgtctga atcattctta gccacaggaa aatacatttt
    29941 aagtttctga cctgtttctt caatggcatg caatagtcca ccggtataat cttgtctctt
    30001 aaagaatgga atcatttctt ctttaacctt gtcccaaaaa acctgactta catattggtg
    30061 aattccacta tctccaataa tggcgaattg tctgttttca atggcaacat aaatcagaac
    30121 accattgtga agtttggttt catgcatctt gaggttttca aatacttctg ctgcgcggtc
    30181 aataatttca ctctttactt tgctgtcaat aaaaatcctg atttctcctg atgtatgttt
    30241 ttctgctcgt gctattgcat tcttcagcaa cagttgttgt tcatcagaaa agtaatcggc
    30301 agcgttggcc ataactaaaa cttaacctca ggcgctttat tggcaccttt atcagattga
    30361 aaataacctt ttgatgaaaa cccaaacatg ccggcaagca atgtttgagg aaacatacgt
    30421 atgtaagtgt taaactgttg tgcagcctcg ttaaaatctt tacgtgccac agcaattctt
    30481 ctttctgttc cttcaagtgt tgcctgcaat tcaagaaagt tctgattggc cttcagtacc
    30541 gggtaatttt cagtcaccat taataggcgg cctaatgcag tgcttaattg cccttgtgct
    30601 tcctgaaact tctgaatgtt ttcaggtgta agtttatcag gatcaacttt tacttgtgtt
    30661 gctgatgcac gtgcttcaac aactgctgtt aaagtgcctt tttcaaattc tgcagcacct
    30721 ttaactgtat tcactaagtt tggaataagg tccattcgtt gttggtattg gttctcaacc
    30781 tgtgcccatt ttcctttaac attttcatca gcagttacca tgctgttgta ggtacctgca
    30841 aacaatcggt atattataaa tgcaacaaca agtaaaatac caataattac tactgaacgt
    30901 ttcatactat atgatttatg gtttataagt taacgtgcca aaactaaaat gtttgataat
    30961 tcgcttttta ggtaataggt aaaagtttta aaattatagg tgaatagtat tattttgtgg
    31021 ataaggtaaa aatcctctga gtaaacaaat ttcttaacat tgcaattcat tgtttattag
    31081 ctgaatgaga aagacggtag caattgaaat tataggcaaa gtgcagggtg tatttttcag
    31141 aaaatataca cagcagacag gatgtagatt aaacctttgc ggctttgtct gtaatcgtcc
    31201 tgacggttcg gtttatattg aggcaacagg tagcgaattg cagataaaag aatttattag
    31261 atggtgtcat aacggttcac ccatgtcggt agtaaggcag gttgttgtca gggaaatttc
    31321 tgtcattcac agtgagccat ttcaaataca gtagtaagtg caaataaatt gtaaaacggc
    31381 tactttagcc gactcatgag tggaataaaa aaattagccg gtcaaacagt actttatggt
    31441 ctgagtagca tagttgggcg tatgctaaat tatttactcg ttcctattta tacccgcatt
    31501 ttttctccgg cagagtatgg tgtggtttct gaactttatt cttattcagc atttcttctg
    31561 atactgttta cctacggcat ggaaacagcg ttttttaatt tcatgcagaa agaagataat
    31621 aaaaataaag tttatgagaa ctgtatttca ttggtttctt atacaacaac aattctggtg
    31681 ttgctgatga tatttttctc atcgcctttg gctgcaacta ttgagtatcc cggaaaggga
    31741 aattacatca tctggcttgc attaattatt ggatttgacg ccatcactgc aattccattt
    31801 gcaagattgc gacaacaagg caaagcattg cagtttgctt tattaaagat tgccggaatt
    31861 gtgattaata taggcttgaa tttatttttt cttgttggct gccctgtatg gattaaaggc
    31921 aatgatggtc tgttgtcaca acttgcagtt acgttttatt ctccaacagt tggagtaggc
    31981 tatgtgttta tctcaaacgt catagccagt ggattaacca tgcttttact ctaccggcaa
    32041 ttcagcaatc taacctggca tctcgacagt gcgttaatta agcgaatgct gtattatgca
    32101 tttccattgc ttattgcagg atttgctggt atggttaacg aaacatttga ccgtgctgtt
    32161 tataaatact tagctccgga tgccaatcac gctctacgtg atttgggaat ttacagcgct
    32221 tgctacaaac ttagtatcat catgacattg tttgtgcaga catttcggta tgctgccgag
    32281 ccttttttct ttgcgcagca cgataaacaa gacaagaagg aaacatactc taaggtaatg
    32341 acttggtttg ttattacctg ttcttttatt tttttagggg taatgttgtt tattgatgtc
    32401 attaaacttt ttattggtga gcaattcaga tcaggcattg atgtggttcc cgtactattg
    32461 ttggctaatc tttgtcttgg tgtgttttac aatttaagta tgtggtataa actcacagaa
    32521 aaaacacgct atggggcata tttttcaatt ttcggagcag tactgacaat aattctgctg
    32581 tttgttctta taccttcatt tggatatatg ggtgctgcat gggctacttt cattacctat
    32641 tttgcaatga tgattgtatc tttcatcaca gggcaaaaac attatccaat tccttataaa
    32701 gttaagaatg atgtgtttta tgttttgtta tcactaatgc tgttttcact ttcagtttat
    32761 ctgcaacatc aacttgattt accgggttct gttaaatgga ttgtcaacat aattttactc
    32821 tttatttttc cgtttgttgt attcagaatg aatagaggag agttaattaa aaaacaaaat
    32881 gtggaatcac aacagaatac aataggcaga aggaattgaa tgtatatttg acaagattcg
    32941 atatttttgc aaatagtgta atattaaaaa atgcaaaaca atacagaagt aaagattata
    33001 aataaatcat cgcatccgct tcctgcatat gaaagcaata gtgcagcagg aatggattta
    33061 agagccgata taaaagaagc catagtgctt aacccattag agcgtgcact tataccaaca
    33121 ggaatttata ttgagttgcc acttggttat gaagcgcaaa tcagacctag aagtggcctt
    33181 gcagcaaagc atggtgttac tgtattaaac actcccggta caattgatgc agactatcgg
    33241 ggtgaggtaa aagttatttt agtgaatctg tcatctgcag cattcacaat aaatgatggc
    33301 gaaagaattg cacaaatggt tgtggcatca catcatcgag tagtgtggaa agaaacacag
    33361 gagttgtcag aaaccaatcg tggtgcaggt ggttttggta gtacaggtaa ataattgatg
    33421 aatagttttg tcaattaaca tattgttata attactcatt tcagagaata atatttttga
    33481 actcaaatta aattgtgtga ttgaatcatt agttatcata ccaacataca acgaaaaaga
    33541 aaatattcaa aagattatta aagctgtttt taatctgctg aatacttttc atgtattggt
    33601 tattgatgac aactcatcag acggcacttc tgatttggta aagcaactta tgcctcagtt
    33661 tccggagtca ttacatttgg ttcagcggca aggaaaaatg ggtttgggta ctgcttatat
    33721 acacggtttc aaatgggctt tagagcgcaa ctatcagtat atttttgaaa tggatgccga
    33781 cttctctcac aatcctgctg acttacagcg attgctcgat gcttgtaaaa atggtggtgc
    33841 cgatgtagca gtaggctcta gatatatcaa tggtgtgaat gttgtaaact ggcctattgg
    33901 cagagtaatc atgtcgtatt acgccagtgt ttatgtacga ctgattacac gcatgcctgt
    33961 tcgtgatgca acagcaggat ttaaatgcta caaacgcagt gtgcttgaaa cacttgattt
    34021 gtcaaagatt aaatttatgg gatatgcttt tcagatagaa atgaaatttg ctgcctggtg
    34081 cagcggcttt cgaattatcg aagttcccat aatttttacc gatcgcaccg aaggtaaatc
    34141 aaaaatgagc agcggaattt ttaaagaagc tttctttgga gttattcaga tgaaaataaa
    34201 aagctggttc agtaaataca cactttaaca tctgtagcct tttgtggttg ctgtttctct
    34261 atgttaaagc agtcagtata aaattatcat ttgagaaaga agtaaaatca actatacaaa
    34321 tttttcacct ttagcagttt ttacatcatt cacaaactga cgtatttgtt gttcgtcctg
    34381 ctttcgacaa accaataaaa tgttttctgc ttcaacaaca atataatcag aaaggccttg
    34441 caataccacc agcttgtcat gcggcatatt gacaatacaa tttttagtat cgtataacat
    34501 tacatttttt ccaaccacag catttttatt tgcatcgtgt gttatatgct cataaagtga
    34561 gccataagta cctaagtcgc tccagccaaa atcagcactc atgacatata cattattggc
    34621 tttctccatc acaccaaagt ctatactgat gtttgtacat tgcgagtaag cagtcttaat
    34681 aaaagcttct tcgccagctt tgttataaac actgttgcct tcttcgaata aatgatacat
    34741 ctcaggcaga tgttctttta aggcttttaa tattgacttc aggttccata aaaatattcc
    34801 tgcattccag acaaactcac ctgattgcag aaaaaaacga gccatttcaa ggtttggctt
    34861 ttcggtaaaa gttttaacct tgaaaatctt atttgtttca ttgtgttttt catctacaag
    34921 ctgaatatag ccatatcccg tatccggtct gcttggtgta atacctaagg taagcaggca
    34981 gtcattttgt gcagtgaaag aaagtccttt attaatagct tcagtaaaag catcttcctt
    35041 ggtgatgata tggtcagatg gagcaacaac aatattggca tcaggatttt ttgctgcaat
    35101 tttaaatgct gcatacgaaa cgcatggtgc tgtatttcgt cttagtggtt cacccagaat
    35161 attttctacc ggtagttgcg gcagattttt ttttactaaa tcaatatacg tttcgttggt
    35221 aacgatatag atattttctt taggacaaat tttgttgaaa cgattaaatg tttgctgtaa
    35281 aagtgtatga cctgttccta aaatgtcaag aaactgtttt ggaaaagcat ttctgctcat
    35341 tggccagaaa cggcttccta tgcctccggc cattattacg cagtaattgt ttttgttcat
    35401 tattttaaaa aaattgtctt aaattttgtt tgatagaatt taaacgcaaa aaaatgaaat
    35461 taaagtctga cagaatgaat aattgttatt ttttattaac gaatagattt gataattatt
    35521 gatgtcccgg cttttcagca caatacaggg ttaaataaaa aaccggctga aattatcaac
    35581 cggtttatta ttgattgcat tatgcttatc tggaaataac tttcaaacgc tgcacagagt
    35641 tttgactttc aacttttata aaataaactc ctttggcaaa attgctgata tcaacagcat
    35701 gacttttatt gttagccggg aattcatctt tagctatagc ttttccgtta atatcataaa
    35761 tagtaagggt atatgactca tgtgagcttc gattgctgta aataataaat ttcccgtcag
    35821 atggattagg agaaatactc aacgttccaa cactgttgtt taattgagtt aacccagtgg
    35881 taatatcaag gtttacatta tcaacgtaca tggagttgcc atagtcatta atgtttacaa
    35941 attttattga cacatcctga ccaaggtatg cactcaatga aacagtatca ttgcgccatt
    36001 gattggcagc acttggtgca aagttgctgg ttgtggttcc tgttgtggca agagccgctc
    36061 cgatttttaa gtaaccggat gcaacccagg tatttccgca gtcagttgaa atatcaacgc
    36121 gcaacccatc ttcataaaca cttgaatatt gtgcatacgc aacatcaaag gttaaaagtg
    36181 cagcaactgc attggataaa cttacttcat aacttatcaa accgtcttct gctcctgaac
    36241 tattatagga aaaattgttg aagcgggcag cataagtaga agtgcctgcc gatcctgtaa
    36301 tgagagcaga tttttgccag gtataattaa tatttgatgg ttcaatagtc cagtttgccg
    36361 gagggaatgc tgttgcctga aaatcttgtg tcattggtgg taatgaagga gcaagaatat
    36421 taatcattgc agtttgtgtt gaacttgatg ttcctatacc attggtagca tctaaggtta
    36481 catcgtaagt gccggatgcg ttaaactgta cttgtggatt ttgtgatgtt gctgatgtcc
    36541 cgtttacaaa tgtcactgta ctggggttaa aagtccagtt ccaacttgaa ggtgcattag
    36601 aactttgatc agtaaaagtg aaagttttgc ttgtacaacc aacattgaag tttgcactaa
    36661 actgcacaaa aggaggaatg tttggacaat tgaaaatgcc gggttgtttt tcaatagcat
    36721 ttgccctgcg gcctttattg ccattagcca tcactgctct cacactaaac caataggtgt
    36781 tagaggatgt aagattggta aagacataac tgttgtttgt tgttgttgcc acagagtcca
    36841 tatacattgc acctagcata cttacttcat accatgcagc attactcact gctgtccaac
    36901 caattttaat tgagtctgga caagagtaaa taacatttaa attttgtgga ataccaatga
    36961 tggtaaatgt tgtgtcggac aaaccggaaa cactacctct tgtaacgcga actcgggcat
    37021 caccggttgt ggcaatgtta ttaggcacat tccacgtata ttgtaatgta ttctgattta
    37081 cagtagctat gttgttccat gtagtaccat tatcagtggt atattctaca tcaaatgatc
    37141 cgaggccttt caatgcatcc cagcgaataa tttcaggatg accgggaaca aaaccttctc
    37201 ctcccattgg ataggtaaca gttatgtcat catttctgaa ctcataaact acataataag
    37261 tttgtggtcc ttgtggaata gcaaaacctt taagcgaaac aatatagttg cctgcagcag
    37321 gattgtctaa tgtaacttgt tccatgttat tcagatggtc aatacctctt gtagcagggg
    37381 cattcaggtt ggctgcattt ggtgtgggat caagtatcca tggattccaa cttacagaag
    37441 aagggtctgt aacctgcata tcaatatcat taacaagata agtcgaagaa gcaggagatc
    37501 cacctacatc atgccaataa accataacac gcatttgaga agtacctgca ggaacgttaa
    37561 ttgaataagt caatgagtca ccctgattaa tactgtcagt aaaataacgt gcatcttcaa
    37621 ttgtagtaac agcgcgaagt gcatttactc ttccccaacc atatttgtaa tcaggaccgg
    37681 ggtttccgat atcttcagca ccattgagca agcaagcttt aatcaaagca gttggtgcat
    37741 cggaagcaga attaatttgt ttataggctt gaataagttg agtgcagata cctgcaattc
    37801 cgggacaggc agcagaagta ccacccccca cctgataggt gttgttttca tctgttgaca
    37861 tttggtcgcg accattagca gcaatatcag gtttaacacg tccgtcagaa gcagggccaa
    37921 tgctgcttga tggatcaata acttccaatg catcaagatt ggctacagca ataacatttt
    37981 ttccttgctt atatccgcct gtaatatttc cccagttacc acctgcacca tagccacaat
    38041 tggcgccatt gttatttcct gccgaaaaaa caaactcaag ttgtccattg tcatgaatag
    38101 tttgatctcc aaattgtgtg tcggtggtat attcattaca tccctgcgag taacttgttg
    38161 aagccaccac agttccaaga ttgttatagt tagcaacagc atcaataatt tggggataat
    38221 ttccaatatc aaaaacatgc aactgaacac cattggccat accgggatat gcaggattta
    38281 aattacctgc accggcaaga attccgcctg tcatatcacc atgagaagga ccggtatttg
    38341 tagaataatt ggtaattcgt ccagtaaaat caatatgcgg accaacaaaa ccatcatcgg
    38401 ccaatgctgc ggctactcca cttccattgt atttgcgacc cataagcaag tcgttgttga
    38461 tgacattgct acggtgtaaa ctacgtcctt ccaaatcatc tttaacagat ggtggtgagg
    38521 ttaaatcaat aaaaaacaac cagggctgtt gtaaaacaga ggcaactgat tgctgcggca
    38581 tgcgcacttc aaccatattg tttatttcag ttgaacttaa aactttacca taagaagcag
    38641 caattagctg tgcttcctgt attgaaatat ttttctgaaa ctgaagtcgc acatcaacca
    38701 ttcctgctgc aggaacagca taggcaggat aatcgcctaa catgctgaca gaaagttttt
    38761 gttcagagga ttgattggtt acacttctaa cattaaatcc tgagagtaat tgtttgttaa
    38821 gcccaatcgg aattgctgca ttaaaagcat tctttggtaa gtactccaac aatttaattc
    38881 ctgcagtttc gattgcagct ttttcagctt gagtcggtac tgaataaaac tgtatgatgc
    38941 gatagtaata cccattgtaa atatcgtttt ccgatacttt ttcctgaata aatgagttta
    39001 agttttctgt gggtaaaaca gctttaccta aaaacatcag gcgaaagtct gtttgcgcca
    39061 tggcgattgt tgtagtaaac aacattaaaa taaagagaaa tcgttttaac atagattgat
    39121 ttgattttat gaattttaaa taaagggtct aaattaaaga aaacattcgt tttattaata
    39181 attgtttaac aattgagtat caatataaag ggtttcagaa aaacgctgca ttatatcagt
    39241 gttttggtta cttttgcacg cttatgaaaa atcgtctatc ggtttatttc ctattaatgg
    39301 ccttggtgat gaccttaggc tcatgcagta agtttaatcg cattcgtaaa agtggcgata
    39361 tgaacatgaa gtatgatgct gccgtaaaat atttcgaaaa caaaaagtat tataatgcat
    39421 tgcagctttt tgaagagtta atagtcgtgc ttaaaggtac tgataaagct gaaagttctt
    39481 attactatta ttgtttaagc tatttctaca ccggtgatta taccagtgca gcttatcact
    39541 ttaataattt tgcacagaca tttccaatga gtgccaaagc agaagatgcc ttattttata
    39601 atgcttattg ctactatttg gactcaccac cttcaagtct cgaccagcaa agcagcctcg
    39661 atgccatcag acagtttcag ttatttatta ataaatatcc aacaagtcag cgtgttgcag
    39721 aatgtaataa gtatattgat gaattaagaa gtaagctaga agtaaaggca tgggataatg
    39781 caatgcttta ctataaaatg gaagagtata aatcggctat tgttgcgttt gaaaacgtgg
    39841 tgaaggattt tccaactacc aaatatcagg aagaatgctt atttttgtcg ctgaaatcgg
    39901 cttatctcta tgctcaaaac agcatagaat cgaaaaagga agagcgttac aagagtacta
    39961 tagatcatta taataagctg atagagatat ttcctgaaac ggaatataag aaagaagcaa
    40021 gccggataat gtcggcagcc gaagaaaaat taaaagatta tcatactgta aattgattaa
    40081 aacaatgaat agcagaaacg tagcacaaac aacagtaacc cgcgatgtgg ttgactttga
    40141 aaaaaagact gaaaatattt atgaaagtct tgccatcatt gcaaagcgtg ccaatcatat
    40201 tgctgccgaa acccgtgaag aattacacga taaactaagt gagtttgcct cctcaacgga
    40261 tactttggat gaggtttttg aaaaccgcga gcagattgag atttctaaat attatgagcg
    40321 tctgccaaag ccaacattgg ttgccattca ggacttttta gatgataaaa tctatttccg
    40381 tagtcctgca aaagaagaaa acaaagacag cgcagaaaat taaaaccatg ttgcagggaa
    40441 aaaaaatcct gcttggtgtt acaggaagca ttgcagccta caaaacacct gaattagtcc
    40501 ggcaattggt tcaacaagga gcatctgtta aagtaattat tacagctgcc gctaaagatt
    40561 ttgtttcacc tcttgtactt gcaactgttt caaaaaacga agcacttcag acatttactt
    40621 cagaagatca ttctacatgg aataaccatg ttgaattagg attgtggtgc gatatttttc
    40681 ttgtagcgcc actatcagcc aacacactgg caaaatttgc taatgggaat tgcgacaatt
    40741 tattgactgc agtttttttg tcatgtcgca atacagttat ggtggcacct gcaatggatg
    40801 tggacatgtt taagcatgct tcaactcaga aaaaccttga gattattgct aaaaacaata
    40861 ttaaaattat cggaccggag agtggcgaat tggccagcgg acttacaggc aaaggtcgca
    40921 tgacagagcc tgaagatatt gtgcaggaag taatcaatta ctttaatcca tcgttacctt
    40981 tgcgaaacaa aatggctgtt gtgactacag ggcctactca ggaagcgttg gatcctgttc
    41041 ggtttattgg aaatcattca tctggcaaaa tgggtgttgc tgttgcaaac tgtttagcag
    41101 ctaaaggcgc atcagtaatt ctaatttcag gaccatcagt tagtttagga aatttgaata
    41161 agtcaataaa ggtaatccat gttacctcag ctgctgatat gcttaaggca tgtaaacaaa
    41221 cacaacctga tgccaacatt atggtcatgg ctgctgctgt tgcagacttt aaaccagaag
    41281 gtttttctga gcaaaaaata aagaagaaaa gtagtgaagg actaaatatt cagttagtgc
    41341 ctacagagga cattgcagca gaaatgggca aactgaaaaa agaaggtcag ttgttagtgg
    41401 gatttgcctt agaaacaaat gatgaaacac agcatgcaga gcgaaagctg aaaaataaaa
    41461 acctcgattt tatcgttctc aataaattgg aagaaacaaa tcaggttttt ggttcagatt
    41521 ttaaccgcat caccatcatt gataaaaacc ttgtgacaac accatttgaa aaaaagtcaa
    41581 aagatgcagt tgcatcagac attgttgaga aaataattac tttagtgcaa tgaagaaaat
    41641 tttcggtttt ataatattga tgatgagcct gaatattgtt caggcacaag aactcaactg
    41701 ccaggtaaat gtgatggcgc cacaaattca gaatgtagat aaaaaggttt ttgaaacact
    41761 gcagcaatct atttttgaat ttatgaataa caccaaatgg acttcagata aatatctgaa
    41821 tcaggaaaga atagaatgct cagttacaat aacaattaac gagcgcgttt caaatgatga
    41881 gtataaagga agcatacagg taacttcaag acgacctgtt tttcaaacat catataactc
    41941 accaacgtta aattttaatg acgataattt tcagtttaag tatatcgaat atcaaccgct
    42001 ggaattcagt gagaatggaa gcaatccaaa tctgacagca gtacttgcat tttatgcaaa
    42061 cattatactt ggtgtggact atgacagctt ttcattgtat ggcggagcac cttattttgc
    42121 aagagcacaa actatagtaa acaatatgca gaatgcacga gaagcaggat ggcgtgcttt
    42181 tgagggtact aaaaaccgct actggctttc tgaaaatatg actaatcctg tttttaaacc
    42241 ttatcacgaa atgttttatc aatttcatct gaaaggactt gacgtaatgg caaagaaaaa
    42301 agatgatgga ttatcacaaa tttctgaaag ccttgacctg ttaatgtccg tacataaaga
    42361 aaaaccgggt tcgttgttat tgcaaacttt gttttatgct aaagccgatg agattgtaaa
    42421 catatattca ggagcctatc ccgatcaaaa agtaaaagtc agcaatctac tgaatgaaat
    42481 tgatcctgca aacggaacta agtattctaa gattatgggg aattgattgt tgtagaataa
    42541 caaaagcggc tgctctgatt cagagcagcc gcttttgttt ggtaaagaca attttatttt
    42601 ttaacggtta ttttgccttt ggcaatttta gtttccccgt ctttaacggt gtaatagtag
    42661 aggccgctac caacatttcc taagtcccat ttgatggtac cttgtttatc agaaacagta
    42721 aattgcttta gctccttgcc tgacatatca aatacagaca atacaatttg ttcctttatt
    42781 gttggtataa aataattaaa agttgttgtt tgacttgcag ggtttggcac aacttcaatc
    42841 ctcacattca ttgctgtggt gtttgtgttt attgattttg gtgtattgcc atgatacctt
    42901 attctgttgc ttgcttcttc tgttgaataa tcatcttctc tgcacattcc atattagaaa
    42961 cataatgcgt tttgtgccat gccttttgca aaactcaact ctccttgaac gaaactcatc
    43021 agctgattta tttgtgttga atctaacata gacaaatcag agctgtcttg ttgcagttga
    43081 tttctaaaac tataaaaata gcagtagtca ttatatgcat tctgttgatc atcgctcaaa
    43141 gtaaaatcaa caggaataga acttaaaacc aactctgccg atgtcaattc gtttgcggcc
    43201 agataatttt ctattaagtc gtattttgca gttaatgttt gtgctcgggt tagccaataa
    43261 attatttgtc tggaataatc tgttgtgtca ccaataataa tgctatccat atctaatgtc
    43321 attttagcat ataaatcaga aagcagccta tctgaaataa ctgccatttg tgaattataa
    43381 tctgcaagca tattttcaat tgctgtgcgt ggcgtaccat catcccacga tgctctgatt
    43441 aaatcaacca tatattgtga caaggaattt acaatactgt attgcataaa ttcaagtagt
    43501 gcttcatctc ttgtagcatc ggggtttgcc aaacaaacca tcaacagcat ggccggtggc
    43561 attatatcct gaccggcagc ctcacgtaat gcatcggcag aaacgtaagg tgatattccc
    43621 atcaaatggt cgcgcaaatc taccgcattg cttgaccaac tttgttgaat ctgattgagc
    43681 agtatatttg tattacctcc atctaatagc tgattatacg agtaaagtaa atttaagaat
    43741 gccgtctctg acgaatcata actatctgtc atttgctgaa tttcgccatt agtcaataag
    43801 tcattacaat tttgacaaat atgcgaggga catgtgttgg agaatgcagc aagaattgga
    43861 gcaacttgag agttataata ctgaggcgct tttgactgat cgaaataata actgagagcc
    43921 actgctgtaa cattattaat atcagtaaaa gttcctgtta gcccggtata tgaaaatata
    43981 tttccggcag agatttttga tgtaccctgc aaaagtctta ttcctgaaaa tggacttcct
    44041 tttcctgaaa taaaaaaatc aaaattatcg tttgaggtat tctcattgca cagatattga
    44101 aggcctgtta atcttgtatc aatcgggttg cgattgattc cggttgaata atttccgata
    44161 ctcatatctg taaaataatt tttataaatc tgattggcag actgcccgca actatttgtt
    44221 gatattccat acttaaacac cgtagcaata tttggatttg aacgaaagaa atgattttcc
    44281 tctattgtaa aaccactaca aaatatggta ctaatgccaa gctgtacgtt tgctcctgtt
    44341 ttaaaattgt tgccaatata aaaatagttt gattgcaatt ttagattatt gatattatag
    44401 acataaatgc ccgtttggtt atttgtaaag gtggaaccca aaacagtaat tgaattgtta
    44461 ccggctgttc ttttagcatc tatagcatga aacaggttta gaaatgtaca tccatcagaa
    44521 acagtatagt ttgcatttaa cgatttaatt cccatgctca acatatcaaa catagcttgc
    44581 gaattaaaat ttataaaatc gcaatttaca aatcttattc cgaaacagcc atcaatatga
    44641 gcatgtatag gcgtggcttg aaaaacatat tttatgttat tttcgaaggt agctcctgca
    44701 aaataacttt tgtttgaata ttcaatacca ttataaatat tctgataagg caaaaactca
    44761 acatccatta aacaattttt aaattttgca cctgcagtgg caataattat tccgcctctg
    44821 tgtaaattca cataagagcc atagtttctt tgtgcacgtg cgccacacac tgcatattct
    44881 attgttgcat tgtttactat tagtttaccg acaaccgatg gaacaatatt ctgactttga
    44941 ttgttctgat tgccccacac ttcaactcct tgccaggtgt aaggaataag gcaagggtct
    45001 tctaaatagt ctgatgttaa aacggcacca ccatccaaag ttaaagtgct gcccggctca
    45061 ataataactt tagcattggg actgaattta aacatcatgc cattgatggt gaggttggaa
    45121 ttttgtttaa cacgcaattc gttaagcaca tagattggtg ttgacgctcc taatggatta
    45181 tttgttgcac cataactcca tgtagcattt tgattaatgc tggaattatc cggaaagtta
    45241 atggtgttag tagttattac atgcggtatt ggacttgatg ttattaaact gtaatcctgc
    45301 ccatctattt gatcgggtaa tgtgtacatt gtactgtttg ggtttacgcc tccaagattg
    45361 tataaatcgt agggggggtt ggataaggga aaggaatttg gacttggtaa aatttgtggc
    45421 gtatttgcat aaggattaaa agcacctaca ttattagtta ttgaaccggg gtcagcaact
    45481 gcatacataa atccattatt actatgtgcc aattctattt gagaaaatcc aaaattattt
    45541 ggtgtgtttg agttaacgac ccgagtgaaa tcagttgagg gaggaggagt tggatagttt
    45601 gaccaatttg accatggcag attaatataa aaaacaccat cagtgcctgc acccataaaa
    45661 agttttgttg catttgctgg ctgaaaaaat tctacacctc taaagccact tatattattc
    45721 cctgagtaat ttggaatatt gaatttttta taggaattat taatatagtc tccactgcca
    45781 tccaattcta tcagatggta gcggtactgt ggaccggatt gatgtgttac taatgcatag
    45841 cttgcccagg caagccactt tccatcgggc gacaattcaa gctcttttga aaaaacttct
    45901 ccacctgcat tataattagg attggctgag gtaggataaa caggaacaga actactaaca
    45961 ctgccatcat tgtgtataat tagtttttta atcaatcctc ctgtggtatt aataaatcct
    46021 gagccaccta agaaataaag ataacgatta ttagccgtgt caatgacttt gcctacagca
    46081 atggcaccaa attctgagcc gaatggcacg ctgtctattg gcagtgatga taaggaatag
    46141 ctattcatat caagcactgt tttccatagt gcaacaggat aaataagtcc accggctgtt
    46201 gtaaaaatat tgaacttttt atgacagtaa tcattgttgc caaaaggcac aacagcaatt
    46261 tctgcacctc cgtttggaat agcacctatt ttagtgttgt tataatcata aaccgttcca
    46321 tcggcaatat aaaacaataa gtctcctgtg cttgtattat cgtaaaaacc gttggcaaca
    46381 tgtaaagcgg tagcagcggt agtgctacca attgcaggac taatggcggc aggaaaagga
    46441 tttggcactg tcatgttaat tctatttggt gcaatgtacc agttgttcat ttgtgttgtt
    46501 tgcgacaacc ctgttgtgca aacagcaaac ataaagattg caatttgtgt taacgttttc
    46561 attttgtttg gtttttgtag tttaaataat atgttattta taaatactta ttttctgcga
    46621 aaccattttg ttgttcacca ttattgttgc aacatacatt ccattgggca gatggcttaa
    46681 gtctatttgc tcctttacgt tattgcttaa cctgcatgaa tatatacatt gattcatgag
    46741 gttgtaaatt ttaagctccg tatttttttg tggaagccct ttaacctgaa tagtaaaatt
    46801 tcctgaagat ggattgggat aaatgttaaa cttaaatgga ttatttactt ctgcaatgcc
    46861 tgtgcctgag tttacattgc ccatgctgtc tgttttaatt atataagaac caccaatgcc
    46921 attgacaata ccttgcccgc caattatgta tccgttatct ttggtttgtc tgacaaaaaa
    46981 actaaagtca ccggtattta actgaccaaa gcgtcttgac caaagcgaat cgccattact
    47041 atctgttttt aataaataga tgtctcctgc aaaaactccg gttaagataa aacctccatc
    47101 gttagtttga taaatagaac ttcctccaca gctaattgta tcgccataga gttttgtcca
    47161 aagggtatct ccaatagcat tagttttaat aataccaata ttatattgaa atgttgctgt
    47221 gttttcaacg taacctgtta tcacaaaacc actatcattg gtttgtttaa tatcttgagc
    47281 tacttcatga taggaaagat cgtacgattt tgtccacaaa gtatccccat aagtattggt
    47341 gcgaatcata taaatatcag aatggaatga tggtgtgcct atctcacctg ccataataaa
    47401 gccaccatca tgagttttcc atattgagcc gccatcagag ctaaaaggag ttgtatatac
    47461 tttactcgaa agtaaaaacc catttccggt tactgatata aagaaaatat ccggcataaa
    47521 agacggaggt atgtaatatc cagacaaccc ataaataaca ttattaacag aagccatact
    47581 gttttgctgc ggtggtagtg ttggtcctat tccaacagaa aaggttttag tccatagtgt
    47641 atcaccattt gcatcaagtc gcaaaagcca gcttttagaa tttgctcctc catcataaag
    47701 agcattcacc atataaccac tgtctggaat ttgctctaaa taaagtactc cttcaatact
    47761 tgggccacta tatgttttta cccattcaat agttccaatt gaatctaatt ttactataat
    47821 ggcatcgttg ccatttaatg aacatacacc accataaata tacccaccat cataagtttc
    47881 ctgaatgcaa ttggcaccac cactacccaa tgtatcaata attttttcga aagtattttg
    47941 ggcaaatagt gttgtattgc aaaaaagcaa tgtaataaat aaggcaatct ttttcatgtt
    48001 taatacggct taaagttgaa tgtaaatgta taagaaaatt taatagaaat agcaaaaagt
    48061 attttaaata atttatttca ttaactttga tttaccggat agcaagtatt attgacacat
    48121 tctacttgca agtatgtgtg ttgatgttga aagtatttag gcctttgttc aacccaaatc
    48181 accaattgtt tttaattata atacctgaaa ttatggaaaa caaaaaaagc aactactcct
    48241 gattactgct gtcaataact tcgtcagaag gcaagtccac agagttgtct ttgtattgct
    48301 ttgtgtaata aagatatttt ccaacgcctg tgcaggcata gcctgatatg gtgtctcccg
    48361 gcaaaagctg tgttcttgta aaagtgcgcc agcgttttga tttttcctga accgctaatt
    48421 taacggcaag tgtatcgtta catgtattta caaaccaaac tttataaggt tttgttgatg
    48481 caggacatga taatgtttcg gcaccaaatt ttttatttac tttttgtaag cattgtccgt
    48541 agtcttttcc atcctgctta gttttgaaag cagaaaaaag taccactgca ccaatcagta
    48601 ttaaaaaaac aaattcacgc attctgatca tttacaataa ttgtttttaa gaatgatttt
    48661 tgcttcttca ttgcctaatt tttttgattt gttccaatct tcacatgcac ctttaatatc
    48721 gccaagcatt tttttggcaa aacctctgtt gttatatgct tctgcatctt caggtttcag
    48781 ctctaatgct ttattaaaat ctacaatggc tttatcatac ttttcaattt tattatatgc
    48841 actgcctctt gaaacataag cccattcatg attaggttca taaaaaatta catctgtaaa
    48901 gttgccaata gcttcagtat atttttttaa gattgtgttg caaaatcctt gttgtaaaat
    48961 tgcatttaca tttgcaacat ccatgtttgc cacgtaggtg tagtccaaca atgcttcctg
    49021 atatttacca ttattaaatt ttattttacc ctgattcatc agtgagtcaa tgcgcatttt
    49081 aaaatcagct gagtcaacct gtgcatttgc ctgaaaacaa aagccggaca acaatacaat
    49141 gaacaatatt ttagcattat tcaaaaggtt ctgaattttc ataattttta tcatttatca
    49201 aacgtattaa atgttgaaaa gcttttacca atccatcgtt ggttttttgt ccataagaaa
    49261 caggaaaact tatgcggctg taaaaaagtc ttttgcctaa gaactcacgg gtgacacatt
    49321 tcttttgtgg attggtacag ttgataagta acatgcctgc tttagcattg tagtaaacct
    49381 tattggcatc aagttctttt atagacgatt ggtcctccct gtatttagtt tctccgtcaa
    49441 aataacttgc tattaggaca ccatagttga tagataccac atatttccca ccaaatttct
    49501 gattaatata gtcgagcgtt tgttgtactg taatgttttc ctgtgctttg attgcaggtg
    49561 aaaaaacact aagtatgaga agcaaaagaa aaaactttct tcttttactg aactctttat
    49621 tgattttatc gtaaaccacg cacattctta ttcagaaatt aggactatcg tttaaactat
    49681 taactaattt tgtatcggta ttgatgcttt tttaacggaa taaaattaca aatgtttggc
    49741 attgggcaaa gaattcattt agttcctgta gggctttttc tcactatctt catgtttata
    49801 tatggcgatt ccactgcaca gacaaagaag acattaacca tcaatgctaa gtttaagatt
    49861 gatggaggag gaaatttaag cgatgcctac atggttttgc agcgtgatgg tcagtcaata
    49921 gaaacactgc caggccagtc atcctatact ttaaatcttg aaataggggc agattatatt
    49981 ttatcattta acaagcctgg atacatcaca aaaaagataa atattaatac aacaggtgcc
    50041 gatgaagaaa ggattgaaca gggttttgaa tcctatccct ttacggtagt gttgatgaag
    50101 caatatgaag gcgtaaatat tgttgttttt aatcaacctg ttgcgaaaat tacttattca
    50161 aaaaggtttg atgagtttga ttacgatact gattacacca agtcaattca gtcggcaatt
    50221 caaaaggcag aagatgattt gaagaaagca gagaaggagt ttaaaaagaa tccgcccaaa
    50281 cctgccaaag acagtacttt agttgcaaac aaacctaagg cagaagaagt aaaaccgcag
    50341 aaagaaaaaa ccaaaactgc accacaggaa gaaccggttg ctgtaaaaga aaaaagaaag
    50401 gatgatgatg cgaagaatga gtctaaaaat gccaccaaaa atgctttagc tactggtgga
    50461 caagaccaac cgctaaagcc aacaaaaggt ggaggaagtg atgatcccga aggtaatctc
    50521 gacagagaaa aaggtgcaga tcaaaaatca gatatagacc tgactgttgg caatgatgct
    50581 attaaaaata aaattagttt aacaaagggc tctgaccagt caccggtaga ggctcctgct
    50641 gagtcacatc cggtagttat taaagctgct gaaaagcctt tgcctgaaaa accaaaagag
    50701 gtagtagttc atacagcacc cgaacctccc gaaaatgttt cagtaagaag agaacaaatt
    50761 gcagaggaca aaaaaactgt ttacaatgtg tgggttacta aaggcaataa aacagtgctt
    50821 tatagtaaag tcgtttatgc atggggtggt gtttactttt ttcgcgacct gaacacttct
    50881 atttctgaaa acatgtacaa acaagccgat gcaggattag tgaatttcta agcgtttttt
    50941 caactgaaaa aacgcattag tttaccttac gttttcagac attcacccaa cttaatatgt
    51001 tttggagttt agtggctttt aaattgcctt gagaaaataa acacagtaat aaaatgttga
    51061 atacatattt ctgcatgaat ctgttcaagc cacaaaatga cagtcaggtt tcattacttg
    51121 aagatattaa aacactgcgt tctataaaag ggaacacatc acggtttact attcttcgtt
    51181 ctgtagctaa aacaagaaaa cacacacgta cagtctatcc tgatggatgg agatttgaat
    51241 agtccgtaga aacattattg cttgacaaat aatctgctat tgctaaaggt attggcatta
    51301 atatgtagcg tgtaaaggcc tttggttaaa cggctgatat caattttatg ttttatgttt
    51361 ttgtttattg gcaatgatat tattttgcgt ccatacatat cagaaatttc aactgtagca
    51421 ttggttaaaa tattgagttg tgttaagtca acaactattt catctgtggc cggattggga
    51481 taaaggttca aagaactttg taaatcggct aatccaacag ttcgggaaat ggtgtctgaa
    51541 aaagccatac aaccattggt atctgtaaaa gttaaaaaat aataaccatt ggtgctgaca
    51601 ggcatgtagt tgaatgctga aggaatgtcg gaataagtta ttccatcaac agaaaaatac
    51661 cagatgaaat tactgccatt tatgttggat agaagggtgt cgttggctgc agaaaaggta
    51721 agttgtgcca tttgctccgg ataaacagtt acagatatag gggcactgct tcttgaacaa
    51781 cctgacaaat ttgtgattac aacaaaataa ttgccactgc tatcaatggt agtacaatta
    51841 agcgcagacc cattcgacca gagaaaagtg cttgtgagag aacctgcaat ggaacataac
    51901 tcaacactgt cgccatggca aaatgttaag ctgccattaa tgttgatagt ggtatcggga
    51961 aagtcttcaa cagccatgac attccaaata atggagttgg tggatgcgca acctaactga
    52021 ttggtaacaa tgcacggtgg acaagggtaa ttcattgtgg attgatagcc atagacacat
    52081 tctgttgtgt ctccgttgtc ccacaagtaa cttataattg tgttggtagt tgaagtatct
    52141 gttgcacaaa aaataatagt atcgccagga caaaccccca atgcctgtat acttacaccg
    52201 gcaaaaggaa tttcgtacga gtcaaccaaa acactatcta ctgccacaca accgctactg
    52261 tccgtagccg ttacaacata atatccattg ttagacaaat cttttgcaga tataaaggaa
    52321 ccattgcttg cattgtagtt aaaatttggt ggacagttgt tgtttgggca tgtccattga
    52381 taggttgtag taaaattatt agggaaggat tccagaatga catcttttcc ggcacatcgc
    52441 ccttgtgaac ctacagagta ggttactgaa gcttgtactg tacatgttgt ttgagcggtg
    52501 gcattaaaaa acgccattaa cagtacaact gctatagtta gttctttttt cattgcttga
    52561 tgttttattg ttagcaataa taatactaac aagtcagatt aacaaatttt cagcaatgaa
    52621 aactcaaaaa taagcgttaa gaaagtttat tttactgcag cctttaccac aaacaatgct
    52681 ttaagtttat ccatacagaa atcaatttca gcttttgtgt tatagcgcga aaaagaaaaa
    52741 cggacagcag gtctttcagg gtcagaatta attccacgta atacatgtga accaatctca
    52801 ctacctgatg aacaggcact gccgcctgat gctgcaatac ctgaaatatc aagattaaaa
    52861 agtaacatgt cttttccttc aaaaggtgga aatgaaacat tgagtacagt aaataaacta
    52921 ttttcactca catctccatt aaaactcact cctggaattt catccagtag ctgttgcttc
    52981 atatactctt ttaaacctaa tatatgtttg cgttcttcgt ctaattctgc ataagccaac
    53041 tccaatgctt tagccatacc aacaatacca taagcgtttt ctgttccacc acgcatgttg
    53101 cgctcttgtg caccgcctgt aattaacggg tgtatcttta gtttgtgatt gatgtaaaga
    53161 aagcctacac ctttggggcc atgaaattta tgcgcagcac aggtgaaaaa atctacattc
    53221 aggtttgata tatccagagg aatatgaccc attgtctgca ctgtatcgca gtgaaaaatt
    53281 gcattgtatt ttttacataa ttcacctatg aggtaaatat tattaacagt tcccaattca
    53341 ttgttggcat gcattaatga tacaaatgaa cgtggattgt tttttagtaa ttcttcaaga
    53401 tgctcatagt cggcttcgcc tttatcgtta agttttaccc agctaagttt aatgatacct
    53461 tctttttcta aatcttccaa agtatgcgta acggcatgat gttcgatggg agaagaaatg
    53521 gcatgtgtaa ttttgtgatc gtgaatggtg caacgtatag ccatattgtt agcctcggtt
    53581 ccacctgatg taaaaaatat ttctgacgga gcaactttta ataatgtagc aattgttttt
    53641 cgtgctttct caattgccga tcgcatttcc cttccaaaag aatggatgga tgatggatta
    53701 ccatatacct tttgcattac aggtagcatg gcttcaataa cttcctgtct tagtggcgtt
    53761 gttgcagcat tgtcaaaata aactttcatt gtttgagtat tgattaagtg tgatgtgggt
    53821 caaacttttg cagggtggct aattaactct ttaatatctg aaataatttt ttcagcaaga
    53881 ttattggcag tagtgatgct gtcactttct gcataaatgc ggatgatagg ttctgtattt
    53941 gatttccgta ggtgaaccca ctctttatca aattcaattt ttaatccgtc aatagtattt
    54001 attttatgtt ttttatattt ctcctgaata gattctaaaa caccatcaac atttatttca
    54061 ggagtaagtt caatcttgtt tttagaaata aaataatcag gataagacga gcgaagcata
    54121 gaaacagtgt ttccatattt tgcaagatgc gtcagaaaaa gtgcgatacc tgcaagagca
    54181 tcacggccat aatgaatgtc aggataaatg actccgccat ttccttcacc accaataact
    54241 gcatttttag ctttcatcat ttccaccaca ttaacttcgc caacggcaga tgcaaagtgg
    54301 cttccaccgg ctttttcagt cacatcgcgc agcgcacgtg ttgaagaaag attacttacc
    54361 gtattacctt ttttgttttt cagaacatag tctgctactg ccaccagggt atattcttcg
    54421 ccaaacattt caccatcctc acataccaat gcaagacgat ctacatcagg gtcaacagaa
    54481 attcctaaat gtgcttttga tttaataact gttttgctga gttctgtaag atgctcgggc
    54541 aatggctcag gattgtgtgg aaactgtcca tcgggagtgc agtatagttc tattacttct
    54601 tcaacaccaa gtgcttttaa taatggtggt aatgcaatgc cgcctgatga attaacgcaa
    54661 tcaataacaa ttttgaattt tttagctgct atagcttttg tgtcaactaa tggtaaagca
    54721 agaatggctt caatgtgttt gtcaatggca tgtgtatcag tagtcactgt tcccaactga
    54781 tttacattgg caaactcaaa cgattcctgt tctgcaattt tcagaagcgt ttcgccatca
    54841 gcagcagaaa taaattcgcc tttgctgttt aacaacttca gtgcattcca ttgtttggga
    54901 ttatgacttg cagttagaat aattccgcca tgtgcctgat gaaatgtaac agccatttca
    54961 actgttggtg tggttgataa acccacatca atcacatcaa gtcccatagc agtgagtgtg
    55021 ccacaaacta agttatgtat catgggcccg ctgatgcgtg catcacgacc taaaacaact
    55081 ttgcattttg tttttaccgg aaattgtgct gcaagccatg tggcgtaagc agcagaaaat
    55141 tttactacat caacaggggt gagtgactct cccgatttac cgcctatagt tcccctgatg
    55201 cctgaaatag attttattaa tgtcacaatc tttgcttttt ttactgtgca aatatatcaa
    55261 aagagcgggt aattttatga atttaactga tggttttcct gaatatcagc aaattatact
    55321 ttctagcaag aattactatt ttcaaaataa ctgcccaatc ttcttagcca aaacttctgc
    55381 aatttttcga ttcgattcat gtgtccaccc tgcaatatgt ggagttagaa tagttttatc
    55441 agattgtatc agatattcaa agggctcagg tagtgtttct aatctttcaa atgatgaagt
    55501 ttcgtattca tgtacatcaa gacaggcacc tttcaccttg cccgttttga gatgcttaac
    55561 caaatcggca gtgttcaggc atttgcctcg tgcagtgttg atgatgtaaa tttctttttt
    55621 gcactgactg ataaatttgt cgttaatcat gtatttagtt tctgcagtca atggcacatg
    55681 cagactaata atgtctgatt gctcttgcag ttgttcaaca gaaacttgtt ttataaaagg
    55741 atattcagca gcgtcaatcg ttttgtaagg atcgcaagct aaaatgttca cctcaaatcc
    55801 ggttagtttt tttgcaaaag aactacccat attgccaaag ccaatgatgc cgacagtttt
    55861 gccctgtaat tctgtaccac ggttttcttc tctcagccag atgccattac gtacttcttt
    55921 atcagacttc agtagtttat tcagaagagc aagtaacatc attaatgcat gttctccaac
    55981 agcatcacag ttagcttcgg gtgcattcag acatttaata ttttttgaaa cagcataagc
    56041 aacatcaata ttttccatgc ctgcacctcc acgggcaata aactttaagt tagtagcatg
    56101 ttgtaacagg tctttgttta agggtatgcg acttctaatg gcaataccct catagttgcc
    56161 tatggtttgt tgtagctgtt caaggctcca ttcatatcct tcagtacaat taaatcccaa
    56221 cgtagtaagc ttgggaataa aagtttcatg aaaggtgtct attaataaaa tttcttttgg
    56281 cattattttt aaaattgaca tgaaataaaa atcatccgaa tgtgcaggat gatgttgtct
    56341 gataagttat ttttattctt aggattacaa gtttaaaggt acttgtcatt gtttccaact
    56401 gccaatttgc tgtttcttga ttcgtaacct cctttaccat tcagaataaa agttgatagc
    56461 acaaaggtaa tttctaaaca ttttgtatcc aaatagctga aataacagca aatattcact
    56521 taggaagaaa gaaaatggtt gacaatgctg aaaaggtttt ttaaattgcc gcaaaaagaa
    56581 gatcgtccat tatcagttaa ccaataaaaa cagagaacca ggtttgctga tagatatctt
    56641 gattatttaa aatgaaatat tttttgtttg aatgatgtaa ggtttacgga aaaatcttct
    56701 ggttatgggt aactattata caagtgtata ggtataacca tcaccaacaa aagaataaat
    56761 cgttatgcac gtattagaca tattactttt aggaggagct gtagtctgtt ttgttctggc
    56821 atatattatt gcttcgcgtc cggcacgaga gttgcgccaa agcagtaaat ctaagtcaaa
    56881 atggaatcat gtcaatatgg attcggcttc gtcagttcat tcccccgaaa tgatgaaata
    56941 tgtccatgac aaaacaggtt aactcacctt actccagtta ggttgctctc gcaactgatg
    57001 cagatgacga taaaatattt tattttcttc tgaattcagt tgtgggtctt cgttcagtat
    57061 gttgatgact gcatttcttg catgttctaa gatagcacca tctttggcta actcagcaat
    57121 tttcagattc agtaatccgc tttgctgtgt tcctgtaata tctccggggc cacgtaactt
    57181 taaatcggtt tctgcaattt gaaatccatc attggtctgt accattgttt tcaacctttc
    57241 acgggcatcg gcagataatt tattccccga cattaatata caaaaagact gttctgcacc
    57301 gcgacctact cgaccacgca actgatgcaa ttgcgataat ccaaaacgtt ctgcattttc
    57361 aataatcata actgtagcat taggcacatt aacacccact tcaattaccg ttgtagcaat
    57421 catgatatgt gttttgccgt ttacaaatcg ctccatctcc tgttccttat cttttgcctt
    57481 catctttcca tgcaccatgc tcacaaaata ttccggcagc ggaaaatctc tttgaatact
    57541 gtcataaccg gccatcaggt tttgcagatc taatttttct gattcatcaa ttagcggaaa
    57601 tacaatatag atttgtcgtc caagtttaat ctgctctttc ataaatccaa taacacgaag
    57661 tcgtgatgat tcaaaaagat gcatggtttt tattggcttt cttccggcag ggagttcatc
    57721 aattaccgaa acgtcaaggt cgccataaag tgtcattgct aaagtgcgtg gaatgggtgt
    57781 agctgtcatc accagcacat gaggtggttg ttgccgcatc cagagtttgg cacgttgctc
    57841 cacaccaaaa cgatgttgtt catcaataat gactatacca aggtcattga attttactct
    57901 gtcttcaatc agtgcatgtg tacctatgag aatatgcgtt tcattggaag aaattctttc
    57961 tataatggct ttctttttcg attcaggaaa agaacctgtc aacagttcaa cattaacagg
    58021 taaattgctg agtaatgatg ttattgtttg aaaatgctga gcagctaaaa tttctgttgg
    58081 tgccataagg caggcctgct gtttgttgtc taatgccaat agcattgaaa aaagtgcaat
    58141 gacagtcttg ccactgccaa catcaccttg caacaggcgg ttcatttgtt ttccgctgat
    58201 agtatctttt ctgatttctt tcaacactct tttttgtgcc tgagtcaatt caaaaggaag
    58261 atggtcattg aagaatgtgt tgaagaggta acctacttcg gtaaacatac gcccgctttg
    58321 tgtggaagag cgtttttgtt tattgagtaa taatttaagt tgaatataaa acaactcttc
    58381 aaattttaag cggaagcggg cttgacgaag taagtcctca ttttccggaa agtgaatctg
    58441 aatcaatgca tcgcgcaaag gcatcaggtt aaaagtcttt atcagctgtg acgataaagt
    58501 ttcttcaatt gtgggaggaa ggtctttaag tgccatgcgc atgattcgca tgatgccctt
    58561 actgtctaga ccttttactt tcgatttttc tgttgaataa taaaacggct gaaaaccttt
    58621 gaatggttgt gctgtaaatt tttcaagttc ttctatttcc ggatgaataa tactccactt
    58681 accgttaaat aaagcaggct tgccaaaaac aacataactt gtcccggctt ttatggagtc
    58741 gcgaatccat gttattccct gaaaccaaac taaatccatg ctgccacttg catctttaaa
    58801 aactgcttgc agacgtgtag ttcgcttatt gccgaaagca gtcaatgaag taatcacacc
    58861 tttaatctgc acataaggca ggtcgtcatt cagttgtgca atggtgtaaa attttgaacg
    58921 gtcaacataa cggaaaggaa agtgatgcaa caggtcagaa acgttatgaa cctccaactc
    58981 cttgcgaagt actgctgcac gttgcggacc aacggtttta acaaattcta tatctttttg
    59041 aaagtatgat tgcacaggca caaagataat tcagaatata ggacaaaaag attagagttg
    59101 ctgccaatat aaatacaaag aaaatataaa tattaaaccc ttgtttactc tgcacttctc
    59161 gctcagtaat ttcattgcag cgaaatgaaa ttactgagtt gtgtatttgt ttttacaaaa
    59221 gagtaaaaat aaattcgggg tttctaatca atgttaaata ctatagatat tgtgatttta
    59281 aaaaagtagt acgtcattat aattaccttt tatttcacca ctattttccc tctgtatgct
    59341 tttcctgaac tattaggtgt aagttcgtaa atgtataatc ccgcattaaa cttgtctaac
    59401 gaaatcaata cttcgccatt ggctgtttta ctgtggtgaa ctattttccc tgccattgaa
    59461 taaacggaca gattatatac actgccactt atattatcaa atttcaacaa cactttttca
    59521 gaggcaggat ttggcataac tattacatct gatttttcgg gggcaagttc attcagtcct
    59581 acaggtatga catacaccat agtgtttagt gtagtgttgg ttattacagg ttcgtttagg
    59641 tcaaaataaa tatcagctgt attattaatc attgttccgg caataagtcc gctgtctgcc
    59701 tcaatagaat atatcacata accatgactt gcaggctcgt ttgtattact gtcaggaagt
    59761 aaaatattat taaaagtaaa tagcaatgcg cctgttttca tatttaaagt gggagtcacc
    59821 gtatgactgt aatcttcaac ccggaaagtt tgccaattta attcggggtc taaaaggtct
    59881 ctgatttgaa ccgtaaaagc agtgtcgttt cctgtattct gaaaacgaac tgtgtagtac
    59941 aaccgttcgt tcattaatgt atagttttga ctttgtacac ccggtggggt cactgcttta
    60001 tcgttagggt caaatgaaca aagaaccatt tgtgtatttg tgcgcgaatg attgagtaca
    60061 acattactac catcggtaag tctgataatt cctgtaacgc taatgatgct tcctgcaccc
    60121 ggaatattga gcacaacatt tttactaaac gtagaaaata gattcagatt ggtaatgttc
    60181 caaaacaaag tatcacccac tgtaaaatca ggagtggcac tggcaacagt gatgttacct
    60241 gaacaaacta tacctagcac tccatttaca gccgtattac tttggttacg gaaagttacg
    60301 taataaggtt gttgtgtatt gcatcttgga agaccggaaa tgataagaga accttcatag
    60361 aaaggtgttc ctgtctgaaa tccgaaatct ttattacttg ctatggaatt atttacacta
    60421 aaattataag tagcaggtgt tgatgttagc gtatatgcaa ccgtaggtac ataagttaat
    60481 gttttacttc cgttgctgga tcctatttta tagtcgccat tggcatctga gatgcctgta
    60541 atactatcgg gcatcaatgt tacatactga tttggaatcg gaaaatctcc cgcatcaaaa
    60601 acgccattgc tatttgtgtc aaaataaatt tttccgttta caatagactg tgcaggactt
    60661 atggcattag aaaaaggtaa aatatgtctg ccaccaaaag ggcttatggt ttgaatggat
    60721 aaatcatata gacccggtaa agcattggta ggaatattaa gggtgatttc agcagttacc
    60781 tcgtccaata tctgtacttg ggtagtcaga ttagcgctat aatgattgcc tgttccgtgt
    60841 tttactatgg atgccgattg aatatttcca tacgtatttg cactcgtttg aaacagtgag
    60901 tcggaaacga tagtaagtgt tacagtttgc ccttgaacca atacagtagg ggaaacagaa
    60961 atcaagccac gctttactcc gaaatcattt cctgaagaat tgttattatt gatagttaca
    61021 ctgtgattag gtgaggaagt tgaaaacaaa aatatgatgt taggattgtt tacccatgag
    61081 acagtgtagc taccattggt aacagcaatt gtatagttac cattagcatc tgtagtatat
    61141 acatggtttt ggggcatgat tttaactgat gatcccgcta ttcctgattc tgttgcatct
    61201 tttacaccgt tattattcag gtcttcataa acctgtccgg ttatgtaacc atcaggcgag
    61261 cctactctaa cagcagaagg taatatgatg tcgtatgaaa gtccttgaaa aggatcaaaa
    61321 ttcaaaacat ataagtcata gaaccctatt ggaatgcctg ttgaaggaat ttgaaagttg
    61381 cctgtaaaat gttcatcatc tattacactt atgttattca taggaattgt cacacctcct
    61441 aagtataaat gccctattgt agaagaattt tgaaataagg tgtttgcacc tgtaatggtt
    61501 gcactgagtg tttgacccaa ggctacagtg tcgggagtaa tagaaactaa taccggttgt
    61561 tgtgcttggc ttaacgtgaa agtgctaatc aggagtagca aaatgtaaaa tcttttcata
    61621 gatttaaatt taatattgta gaaaaacaaa attatagtat aaatttatga aaaagaatag
    61681 gtgtatctaa tctttctgaa gtcattacac atcagcattt agaaaatctg ttttatgata
    61741 ttcgcatttt gcaaaggtta atgaagggta aacatacctt taggttataa atgccttaaa
    61801 attaaaatcc ccgattgagt gaacaaccgg ggaataacat taaaaattac aaataatcta
    61861 taggattatt actgttgctt tttaacattt acggcattta agcctttttt gccttgaact
    61921 acttcatagg taacaaggtc attgtccgcg atgcggtcaa ttaatcctgt ctgatgaaca
    61981 aataggtcac gaccaccttc atcttcaacg ataaatccaa atcctttgga gttgttgtag
    62041 aattttactt ttccggtttt cattaaataa agaattaaat ttgtgaatta atgctgcaaa
    62101 tgtaaactaa attagttacc atctcataaa aatcaaaatt atttattttt cgaatcaaac
    62161 ctatggcttc atatagcgga aatatcatag atatctttaa ccgaaggatt ttccccggaa
    62221 cggttcattt tagcaatgga ataattacag aaataacaga ggataatgct gtttatgacc
    62281 aatatttgct gcccggtttt actgatgctc atatccatat cgaaagcagc atgcttgtgc
    62341 cttatgaatt tgctaaggta gccctgattc acggaaccgt tgccaccgtt agtgacccgc
    62401 atgaaattgc caatgtttgt ggtatgaagg gggtggagta tatgattgag aatgcaaagg
    62461 atgctaaatt gaaatttaat tttggagcac catcctgtgt gcctgctact tcttttgaaa
    62521 cggcaggtgc aacgctaaat gctgatgatg tggcacaact tcttgccaga cccgatattc
    62581 actatctgag tgaaatgatg aactatcccg gtgtgctttt tggcgatcaa gaggtgatga
    62641 aaaagattaa atcggctcac aggatgaaca aagtggttga tggtcatgca cccgggttga
    62701 gaggcgatga tgcacgcacc tatattgctg ccggtatcag cacagaccac gagtgtttta
    62761 ctctggatga agcgttggat aaggtaaatg ccggaatgaa aatattgata cgcgaaggct
    62821 ctgctgcacg aaattttgaa gccctgcata gcctaatcac ttcgcatacg gcaatgacca
    62881 tgctgtgcag cgatgacaag catcctgatg aattacttca tggacacctg aatcaattgg
    62941 tggttcgcgc cctaaaaaat ggacacaatc tttttgatgt gttgcagtgt gcctgtgtta
    63001 atcctgtgtt gcattatggt ttatctgtgg gattacttcg tgaaggtgat tcagcagatt
    63061 ttattgtcat caacaacccg gaagagtttt tggtctcaca gacatggatt gatggagaat
    63121 gtgttgctaa agatggacga acaacattga caaaccgtag tcataattca ataaataatt
    63181 ttcatgcatg gaatgttacg gtccaggaca ttgaagtgaa aaaaacttcg gaaacggtta
    63241 aagttattga agcattggat gggcagttaa ttactaattg cattcaggct aagccattgg
    63301 caagagacgg acagtgggtt agcaatccgg agggcgatat tcttaaaatt gcagtcatta
    63361 accgctatag taaacaaaaa cctgtggtgg gatttattaa aaatttcggt ttaaagaatg
    63421 gagctattgc ctctactgta gcacatgact cacataatat tattgtcacc ggtgttggtg
    63481 atgccgatat tgttgaagct ataaatttac ttatgcaatc gggtggagga ttgagtgcag
    63541 tatcgggaac agataaaaaa gttattgcct tgcctgttgc cggattgatg agtgataaaa
    63601 gctgtgaaga aataggtcgt gattatgcag ctatagatag tatggctaaa caaataggtt
    63661 caacacttag agcacctttt atgaccctat cttttatggc attattagtt attcctcatt
    63721 tcaaaattgg cgacaaagga ttgttcgatg ctgcaaagtt tgaattaact acgcttgagt
    63781 aaatttctaa gatatacgtt gaattgttac ttttacaaaa aaacacaatg tggcaggaag
    63841 taaataattc gttagaacga agttttgttt ttcatgattt taaacaggca tttgcattta
    63901 tgacacaggt agcttttata gctgaaaaaa tgaatcatca tccctactgg tgtaatgttt
    63961 ataataaagt caccattaag ttaaacacac acgaagcagg aagtgtggtt actgaaaaag
    64021 atcataaact tgcagcagca attgatgttg tgttcaatca aataaatcaa aaagtatgag
    64081 ccgattaaaa aagtttttta tttgggtagt tgttcttgcc ataattggaa cagcattata
    64141 ttttcttgcc ggaagttata cctattccga aggaacacaa acgggctact tggtaaaatt
    64201 ttcccgtcaa ggatatgtgt ttaaaactta tgaaggtgag ctaaatcttg gtggcatgaa
    64261 tgctgaaagc aattctgttg taaataacct ctggcatttt tctgttgatg aagatgataa
    64321 ggctgctatt gacagtcttc gtgtcagcga aggacgtgtg attaaagttt tttaccgtga
    64381 aattgtacgc aactatgcct ggcaaggcga aaccccgttt tttgtttaca aagtagaaaa
    64441 agtaaagtag gcttttatcc taaacgatgt ctgataatgg aatcaatttt tgccattaat
    64501 tgcgaaggtc tgtaggtacg ccagttcaga ttatcaagtt caccaccaaa caataaatag
    64561 tagtcaatat tggcacgttg catttcagac aaaacatgat ctctgaaatt gatagcagca
    64621 atccatcccg tttcatttac ttcttcaaac cattcctgat agtaacaatc gtctgagttg
    64681 tgaaaattga gtacgttttc gccaataagt ataaagcggc ttatgccatg ctccatcaga
    64741 ttttctatca catctctttt taatgtcata atatcattat tgatggcatc attccattcg
    64801 cccatcagtt caatgatggc aaatgacttt tcatagtcgc aataaattat ttttatgtaa
    64861 agtgtgggcg aaccaaaatc gtcccattgc ggatgaataa agtagttgta aatggtgttg
    64921 ctgtattcaa actcactgta aacccttcca taaaaaggac tcaactcatc ttctgccgat
    64981 gagtataaat ctacccagtt ataaaaaggc tgtatgtcct gcattgtttt attagaaaat
    65041 gcaaaattac aacagacaat ttcaaactac aacagtttat gctttttgat tttttattgc
    65101 tgcccgcacg ctaccatatt ttttcagtaa cagggctgct tttttttcgg gaacttttag
    65161 ctcatccatt atcattctag tgccgcgact gactaattta ttgttggtaa gctgcatgtc
    65221 aaccatttta ttgcctttaa cacgaccaag ttttatcatc actgccgtgc taatcatatt
    65281 caggactaac ttttgcgcag tacctgcttt catacgtgtg ctgccggtta caaattccgg
    65341 tcctgtaata atttcaatcg gataatcggc ttgcttcgca accggtgaag cactgttaca
    65401 tacgatacat ccggtagtga tttttttctt gcggcattgt tccaatgccc ctacaacata
    65461 gggtgtagtt cctgaagctg caatgcctat tacaacatct tttttattta ttttaaattt
    65521 tttcaaatca ttccatccac ctgaataatc atcttcggca aactcaacag ctttacgaat
    65581 agcagcatct ccaccggcaa tgagtccaat tacttttcca tgttcaatac cataagttgg
    65641 cggacattcc gatgcatcca caatacctaa ccttccgctg gtgcctgcac caatataaaa
    65701 taaacgtcca ccttttttca tccgtgtagt aatcactttc accaactttt ctattgcacc
    65761 aatatttttt tttatctgtc gcggtacagt atggtcttcc ttattaatgt tattcagcaa
    65821 ttgttgtatg ctcatcttct ccagatgatt gtagtgcgaa gttttttcag tagttcgcag
    65881 gctttcggat tttttattca tgaaaaacgg attgtcggtt gtgttttagt tcatatacat
    65941 gctccttgat tttttcagca gtttttccat catcagttcc agaaaggtct tgtggaagaa
    66001 taggtttgcc gaaggtgata gagatagttc tgccgcgttg aaaatacatt tcatccatca
    66061 ggtaaagcat ttcaatattt gctgcaatgc ctatgcgttt acgaaagttg gctaagttat
    66121 aaaaccagtt tgagtttcgc ccatcaatat aaaccggtac aatagggctg ttgtattttt
    66181 ttgctttcac cacaaagctt tttttccatt gcaggtcttt tatttctttt ccttgcttgc
    66241 gcgataccaa tccggcagga aaaatcaaag ttgccatttc agaagcatac aatgcatcaa
    66301 tgtctgcaat tgactgcgaa ctgttttttc catgtttgtt gacgccaata aataaatctt
    66361 taagattgtt tagttgcagc aaaatatcgt tgacaataaa tttcaggtcg cgtctgtaat
    66421 gtcccagtgc ctgcatcaat gccatggcat caagtccgcc aatgggatga ttggatgcat
    66481 agacacagcg accggttttt ataatattct cttgtccata aacttcaact tttactctga
    66541 aattttcaat aatagcatct acaaattcaa acgatgtttt gtgcccatgc tcatcaataa
    66601 atgcattcac ttcggtttcg tgcactatcc gtttgatgta gctaacgata aaccccggca
    66661 gcaggcgata aagttgcgga ttcttacttc ggattacctc actgacatca ataaacttct
    66721 ttttctgctc agtcataaat tctccctctg tcgtatagca tcttttatgg gtacaatatt
    66781 aagcagaact taaaaactgc cttagtctat aaaatcccaa ataaaaatgt tccggtggga
    66841 ggaaaatagc tgaaaaaaga aagttttcaa caaaaatgga aaaaagtggg gataggtggg
    66901 agatttattt ttacattcgc acaagaattt tttagcacac catttaataa tgattaatct
    66961 gtttggagtt tacgagagca ctgttgatgc gaaaggtcgt gtcatgttgc cttcgccata
    67021 cagaaaaaca ttagagaaag ttttagaaga aggttttatc atcaagcaga atgtattcag
    67081 aaaatcactg gagttttttc cgcttcagac atggaatgaa atttcacagc atgtaggcaa
    67141 gctgaatact tttttgaaag ccaatcagga attgattacg ctgttttatt atggtgtgcg
    67201 gagtgtagag ttagataaca acggaagatt tttgattgta aaagatcacc tgacttatgc
    67261 aggaattaaa aaagacattg tgctgagtgg agcaaatgac agaattgaga tttgggataa
    67321 gcgagcttat cataaatata ttaaagataa cagcgacaat ttagagcggt tgatggaaga
    67381 gcggcttggc ggcaaattgc ctgaaaatgg acagtaaggc atatcacatt ccggttatgt
    67441 tgccggaatg tattgaaggg ttgaagatta aagcagatgg aatttatgtt gatgttacgt
    67501 acgggggcgg ggggcattca aaggcaatag cctctttatt gacaacagga aaagtgattg
    67561 cattcgacag ggatgccgat gcagtaaaaa atagtttaac caccaataaa aatctgattg
    67621 tgattcacag cagtttttca aatataacca ccgagctgaa tagcataggc ataacttctg
    67681 tagatggtgt gcttgccgac cttggtgtgt catcacatca gtttgatgaa ccgcagcgtg
    67741 gttttgcttt tcgttttgac agtgctcttg atatgcgcat ggatgtacgt cagcaaataa
    67801 cagccgcaac aatcctgaat acttattcta caaaagaatt gcagaagatg ttttcacttt
    67861 atggtgaggt gcgcaatgca gctactgtag cccgtcagat agtttccttc cgtcagcatg
    67921 ccacattagc aactactgcc gatctgaaaa aatgtgtttc acacttagtg cccaagagtg
    67981 atgagaatgg tttttatgct cgattgtttc aggcactgcg aattgaagtt aatcaggaat
    68041 tgaaagagtt ggaattgttg ctgcaacaac tgccgaattt aataaaagca gaaggacgtt
    68101 tatgtgtgat gagctatcat tctcttgaag acagattggt taaaaatttt atcgcgtcag
    68161 gaaatgtgca aggtgtagta gagcaagatg tgtttggcaa cattacttca aaaacattta
    68221 ctgcaataaa taaaaagcca attgaagcta aagaagaaga aaaaatcaat aatccgcgtt
    68281 caagaagtgc acgattaaga gttgctgaaa aaataaagta acatgaacac cataagaaat
    68341 acagaggaaa agaaggaaaa agaaaagccg aacggcagca aaattaagac tgcccgtaaa
    68401 ttcctttcta tgcttaatgt tattgggctg attgataaaa ggatgttact gcgcatgctc
    68461 ccatttgctt ttttcttaat gggacttgca ttggtttata tagcaaatag ctacgttgca
    68521 gaaaagacaa ttcgtgagat tgaccgcaca tcacgtgata ttaaagagct gcgatcagaa
    68581 tatatttctg taaagtcgga gttgatgttt aaaagccgtc agtcgcaagt agcaaaagaa
    68641 gtgcttccta tgggcattaa gcaactgaca gtgccgccta agaaaatcgt acttaaaaca
    68701 gcaaaagact aagagcgtat ggcagatatt aaaaaggata ttttactgag agtatatttt
    68761 gtttatgcct tgatttttct ttttgctatg gccattatgg gtcgtgtttt tcagttacag
    68821 tatgtcgaag gtgattattg gaaacaacgc tccgacagtc tgacattagc ctataaaacc
    68881 attgagccat caagaggaaa tatttttagt gccgatggaa gtctgttagc cacatcagtg
    68941 cctttgtatg atttgcgcat ggatatgaag gcagaaagta ttactgataa aatatttaat
    69001 gcaggtattg actctcttgc cttgaatctt gctgcattgt ttcaggataa aacaaaagat
    69061 cagtacaaga gaatgcttcg tgaaggcaga cgtgatggtg aacgctattt acttataaag
    69121 cgtaatgtaa gctatgcaga actgaagaaa gtaaaaacgt ttccaatttt taataaaggc
    69181 cgttataaag gaggactcat tgtagaacaa aaaaatatgc gtcagaaacc atttaaggaa
    69241 cttgcttcca gaacaattgg ttttaaaatt ggcaatgttc aacctgttgg tcttgaaggt
    69301 gcattcgacc atagtcttag aggagaaagt ggcaagcgac tgatgcaaaa aatatcaggt
    69361 aacatttgga agccactcaa tgcagaagac gaagttgaag caaaagatgg ttgtgatatt
    69421 tatacttcaa ttgatctgaa catacaggat gttgcacaac agtcacttta taatcaattg
    69481 gtaaaaaacg atgctgatgc aggatgtgct atactgatgg aagttgctac cggtgaaatt
    69541 aaagcaattg taaacctgaa aagagctgac tccggttatt atcgcgaaga ttataattat
    69601 gctattggct atgcttcaga accgggctct acttttaaat tggtgagtat gatggccgga
    69661 atagaagacg gctatattga ccttgaagat acagtggatg caactaatgg aagaaccagt
    69721 tacgcacccg gtttaattat gcgcgactca catgaaggat taaataagat aactgcaaaa
    69781 catgcatttg aagaatcatc taatgtgggt gtttctaagt tgattcagaa atattatcgg
    69841 aaaaatcaac aggcatttat tgaccgtgta cataaaatgc attttggtga taaactcaat
    69901 atccagattg caggagaagc tacaccgttt attaagtcaa ctacaagtaa agaatggagt
    69961 aaaatctcat tgccttatat gtctattggc tatgaaacga tggtgactcc attgcacaca
    70021 cttacttttt ataatgctgt tgccaacaat ggcacaatgg taaagccact ttttgtaaaa
    70081 gaaattcgct accgcggaca tactgttaaa gaatacaaac cacaagttgt tgctgattct
    70141 atttgctcaa aatcaacgat tgctaaagca agggaactcc ttgaaggggt agtacaaaat
    70201 ggtaccggac gtaaattgaa agactgcgta tatgccgttg ccggtaaaac aggtacagcg
    70261 cagatggcaa ataataaagg tggttataag ggtagtaaga ttagatatca ggcatcgttc
    70321 tgtggttatt ttcctgctga caaacctgca tattcatgca ttgtagtagt gtataatcct
    70381 tcgactgcgg gttattatgg cggtgaagta gcattgcctg ttttcaaaga cattgcagat
    70441 aaagtttatg caaccaatct cgatatgcac aaaggacttg atgtacaaat ggcagcatta
    70501 ccaaagataa aaacaggtct tactaaggca acaattgcag cgtgtagcaa tgttggtgtt
    70561 gatgtaaaaa cagtgggtac agcatcaaaa tggtcgtcag ttgataagag agaatctgtt
    70621 gcagcattga agccagtaag cctaaaagac aatacggttc caaatgttga aggtatgggt
    70681 ttacgcgatg caatatatat gcttgaaaca aatggtttgc aggttaaggt agttggacga
    70741 ggtgctgtgg tgaagcaatc attacaggca ggaacaagaa tttataaagg tcaggtaata
    70801 actatagaat taagttgatg cagttactga aagaagtttt atacaaagcg ggtacggagg
    70861 aagtcattgg taataccaat gtagctgttt atggtataag cattaactcg cgcgaagtta
    70921 agcccgggga aatgtatgtt gcacttcgtg ggaccaatgt tgatggacat gcttttattc
    70981 tcgatgcaat tgaaaaaggc gcaacttcag ttttatgtga agtgatgccc gacctcattc
    71041 aggcaggtat caattatata aaagtaaaaa atacctatct ggcgctttcc gaagtatgtg
    71101 caaactttta tcatcaccct tcttcttcac tcaaattagt tgggattacc ggaaccaatg
    71161 gtaaaacaac tactgcaacg ttactctata aattattccg ccaactgaat attcgttgcg
    71221 gattaatttc tacggtagaa aacagagtta acgatactgt tattcctgca acacatacaa
    71281 cacccgatcc gataaaactc aatcagcttt taagcatgat ggtgctggaa gaatgtgagt
    71341 attgttttat ggaagtaagc tcacatgctg tggttcaaaa cagaatagga ggattggaat
    71401 ttgccggtgg tgtttttacc aacattacgc atgatcatct tgactatcat aaaacatttg
    71461 atgagtacct gaaagcaaaa aagaaatttt ttgacggtct tcccgagagt gcattcgcat
    71521 taacaaatac cgatgacaaa aacggtacag tgatggttca gaatacacat gctgccaaaa
    71581 aaacttatgg tctgcatggt attgctgatt tcagatgcag aataattgaa aataatttta
    71641 gtggtctgtt acttcagatt gataacacag aagtgttatg taaacttatt ggtgctttca
    71701 atgcctataa tcttttggca atttatgcaa cggctgtttc attggggttg gataagcaac
    71761 gtgtccttac tgcattgagt atgcttgaat ctgtcaatgg cagattcgat tatgttgttt
    71821 ccggttcagg aattacaggc attgttgatt atgcacatac acccgatgca ttaaataatg
    71881 tgctttcaac tatcaacgaa atcagaacaa gaaatgagaa agtgattaca gtagtaggtt
    71941 gtggcggaaa tcgtgataca acaaaacgcc cggttatggc acacattgct tgcgagatga
    72001 gtgataaagt tattcttact tctgacaatc cacgtaacga agacccacaa actattattg
    72061 aacaaatgcg tgccggtgta cctgcacatt tatctgctaa ggtgatggca ataaccgatc
    72121 gcaatgaagc cattcgtgtg gcatgcaacc tcgctgcagc cggagatatt atcctcattg
    72181 caggtaaagg acatgaaacc tatcaggaaa tcaaaggagt aaaacatcct tttgacgata
    72241 aaaaaatatt acaggaaaac tttaataacc tacacgcctg atgctatact acttatttga
    72301 atatttagat caaagtacag atttgcccgg aatgggagtg tttaaataca tctccttcag
    72361 agcagcatta gcaattatat tttcattgtt gttttctttt attctcggac ggacaataat
    72421 tgcctatctc cataaaaagc aagttggaga aaccattcgt gatctaggcc ttgacggaca
    72481 agtgcaaaaa caaggcacgc caacaatggg tggtctgatt attctttcag ctattcttat
    72541 tcctacaatt ttatttgcga agctgcttaa tgtttacatc attctgatgt tgatttcaac
    72601 agtatggtta ggtgcaattg gtttccttga cgactatatt aaagtattta agaaagataa
    72661 aaagggtctt gccggaaaat ttaaagttct cgggcaaata ggacttggtc tcatcgttgg
    72721 cagtgttttg tattttaatg agcatgtagt tgttcgcgaa aaagcaattc gctctgcagt
    72781 tacctggcat cacgataata cattggttga tgatcttcaa agtcaggaag gagtgttgtt
    72841 ttcaactacc aatatcaaat caacaaaaac cactattcct tttttcaaga ataatgaatt
    72901 tgattatgca acattgttgg gatggttggg agatggatat aaaaatcttg catggcttat
    72961 ttttattcct gttgtaatta taattgttac tgcagtttct aatggtgcaa acattacaga
    73021 tggtattgac ggacttgctg ccggaagttc aggtattata ggtttagcac ttgcagtttt
    73081 tgcctacgtt tccggtaata cagttttcgc caactaccta aatattatgt acattccaaa
    73141 tgcaggtgag ttgcttgttt ttatgtcggc attcataggt gcatgtattg gatttttatg
    73201 gttcaacgcc tatcctgcac aggtgtttat gggcgacacc ggaagccttg ccttaggtgg
    73261 cattattgca gtgtttgcta ttgctatcag aaaagaattg atgattcctg ttatctgcgg
    73321 aatttttctc attgagaatc tttctgtagt gatgcaggta agttatttta aatacactaa
    73381 gaaaaaattt ggtgagggga gacgaatttt taaaatgtca ccattacatc accactatca
    73441 gaaacttggt tatcatgaat caaaaattgt aacccgattc tggattgttg gaatcatgct
    73501 tgctgtatta agcctggtaa cattaaaact tcgataagta aatgtcaaaa aacaaacgta
    73561 tagctatttt gggtgctgct gaaagcggag taggcgcagc catccttgca caaaagcaag
    73621 gctatgatgt ttttgtgtca gacctttcgc gaattgccga taattacaaa acacatctta
    73681 cagagcggaa tattcagttt gaagaaggtt gccataccga cagtctgatt cttaatgcag
    73741 atgagataat taaaagtccg ggtatccctg acaaggcagc agttattaaa aaaattaaaa
    73801 atgcaaacat caatattgtt tcagagattg aatttgcttc aaggtttacc aatgctaaaa
    73861 tgattgccat caccggcacc aatggtaagt caacaacaac tttactgacc tatcatattc
    73921 ttaaaaaagc cggagtaaat gttggacttg ccggaaatat cggaaagagt tttgcctggc
    73981 aggttgcaga agaaaaattc gatcattatg tacttgagct aagcagcttt caattggatg
    74041 atatgtacac cttcaaagct gatattgctg tgttactcaa tatcacagcc gatcatcttg
    74101 accgttacga gtattcaata caaaattata ttgactctaa attcagaatc atacagaatc
    74161 aaactgcaga agatgcattt atctattgct ttgatgatgc agttataaaa acagaagtcc
    74221 tgaaaagaaa ccctttggca acatgctatc ctttttcaat cagacaggat gaaggcatga
    74281 gtgcttacgt aaatgacaat aacttatatc tgaacataca caacaactta tttactatgt
    74341 ctattttcga actagcctta cagggcaaac acaacctcta caacagtatg gctgccggag
    74401 ttgcagcacg catactcgac attcgcaagg agtctattcg cgaaagcttt gcagattttc
    74461 gcaatgcaga acatcggatg gagttcgtca ataccgtaca cggtattgac tttatcaacg
    74521 attcaaaagc aacaaacgta aacagtgcat ggtttgctct cgaaagtatg caaaagcctg
    74581 ttgtttggat tctgggtggt gttgataaag gtaatgagta tgaaatgctg aacgatctcg
    74641 taaaagagaa agtgaaagct attgtttgtc ttggcaaaga caataaaaaa atacatgaag
    74701 ctttcggaag tattgtaagt gatattaccg ataccttgtc tgctcaagaa gcagtaagtg
    74761 ttgcgtatca gaaaggcaaa aaaggtgatg tagtattgtt atcgccatgc tgtgcaagtt
    74821 tcgatttatt taaaaattac gaggaccgtg gtcgtcagtt taaagcagct gtaaaagcac
    74881 tttaagcttt aattgaaaca aaaatgaatt ggatagaaac atattttaaa ggtgaccgca
    74941 tcatctggat ggtggtaata ctgctttcgt taatgtcact tatggcggtt tatagttcaa
    75001 caggaacttt ggcatataag tatcaggaag gcaatacaga gtattattta tttaagcatg
    75061 gtattattgt ctttctgggt tttggtatca tgtattttac ccatcttgta aaatatactt
    75121 tctatgcaaa agtttcacgc atagcaattt ttcttgctat ccctctttta ttactcacac
    75181 tacttacagg tactaatctt aacgaagcat cacgttggtt aactctgcct gtaatcaatc
    75241 tttcgtttca gacatctgac ttagccaaga tagccttgtt gttgtatgtt gcacgatcgc
    75301 tatctaaaaa gcaagataac attaaagatt ttaagtctgc ttttcttccc gttattttac
    75361 ctgtgtgtat tatctgcgga ttaattcttc cggccaactt ttctactgca gcagttttgt
    75421 ttagtacctg tcttgtactg atgttttttg gtcgtattaa tttcaagtat atactttctc
    75481 ttgttggtat cggtatttta ggattgggtt tgtttttaat gattgcctca ttttccggtt
    75541 acgaaggaag aattgggaca tggaaaaaac gaattgaaaa tttcagcaat ggaaatgatc
    75601 agggaaatta tcagacacaa caggcaaaaa ttgcaattgc ctcaggtggt atttttggta
    75661 aaggtcccgg tcagagtaca caaagaaatt ttcttccaca cccatattcc gattttattt
    75721 ttgcaattat tattgaagag tatggcttca tgggtggttt ggtaattgtt gttctatatc
    75781 tgttgctgtt ttatcgtgca gtcgtcattg tgcgaaagag cccgtttgct tttgcaacgt
    75841 tgcttgccat aggatgtgca ttcagcttag tttttcaggc tatgataaat atggctgttg
    75901 cagtaaatct tttccctgta acgggccagc cattgccgat gttgagcatg ggtggaacat
    75961 ctatatggtt taccagtatt gccgttggca ttattcttag tgtaagccgg aatattgaac
    76021 aggaagaatt aaaaacagga gtagtgcatg cagcagcctg acattaaagt tatcatcagt
    76081 ggaggaggta caggaggaca tatttttcct gcacttgcca ttgccaatgc aattaaaagt
    76141 gcaagacctc tggctgaaat actttttgtt ggtgctaaag gtcggatgga aatggaaaaa
    76201 gttcctgctg caggatataa aattgaagga ctttggataa gcggaataaa acgcgagctg
    76261 acaatagaca acctgtcatt tccgttcaaa gtgttgtcaa gtatcagtaa ggctatgcat
    76321 attttaaagg agtttaaacc aaatgccgct gtaggtgtag gtggctatgc cagcggacct
    76381 ttactttatg cagcatcatt aaaaaaaatt ccttgtctca ttcaggagca gaattcctat
    76441 cccggaatca ccaataaaat tcttgccaag cgtgttcaga agatttgtgt tgcctatgaa
    76501 ggtatggata agtatttccc tgcatcaaaa attacgctca ccggaaatcc tgtaagagaa
    76561 aatgtgctta tgcttgatgg aaagtttgac gaggcaatgt cgttttttaa ttttaatctt
    76621 tcacaaaaaa cattactcat tgttggtggt agtcagggcg cacgatctat caataaaagt
    76681 attcttgccg gattagaaaa actgaaaaat gcaggagtgc aattgatatg gcaaacaggc
    76741 aaattgtttt atgaagaggc acaacgtgcc gtagcagaag caggctatga gcaagcgcgt
    76801 gtttttgatt ttattgcaag aatggatttt gcttatgctg ctgcaaattt agtggtgtcg
    76861 cgtgctgggg caagtacagt ttctgaacta acgttgacag gaaaaccttc tgttatggtt
    76921 ccgttaccta cagcagcaga agatcatcag acaaaaaata ttgaagccat ggtgaaaaaa
    76981 gatgcagcgg ttttggtgaa agatggcgat gcactgcaac acttagttga tacagcatta
    77041 aagattatta atgataccga tcgtttgaag cagctttcag ccaacactgc agcaatggct
    77101 ttacctcatg cggctcaaaa aattgctgac gaagttattg caatggctac gcagcatgca
    77161 gcacaaaaac ttaaacaggc aacagcgtaa ccatgagatt cgatactata acgcatgttt
    77221 attttgtcgg aattggcggc attggaatga gtgcacttgc acgatatttc aaagctatag
    77281 gaaaacaagt tgcaggctat gacagatcac aaacagtatt aacagatgag ttaattaatg
    77341 aaaatattga cgtacatttt aatgatgatg aacaaacaat acctctagca tttagagata
    77401 caaacagaaa gaataatatt ctggttgttt atacaccggc aataccaaaa aatcattcag
    77461 aacttaattt tttccgtcag caggagttta ctg
//
LOCUS       DAOQNP010000003        69820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_102_length_69820_cov_9.893672, whole genome shotgun sequence.
ACCESSION   DAOQNP010000003 DAOQNP010000000
VERSION     DAOQNP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 69820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69820
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102_length_69820_cov_9.893672"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PKY09_00785"
     CDS             complement(<1..153)
                     /locus_tag="PKY09_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11413.1"
                     /translation="MSAVKYVFVTGGVSSSLGKGIISASLAKLLQARGYRVTIQKLDP
                     YINVDPG"
     gene            423..1790
                     /locus_tag="PKY09_00790"
     CDS             423..1790
                     /locus_tag="PKY09_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11414.1"
                     /translation="MKKLYAFLIAMLISSFTNSISAQSQNALDFDGIDDQVVSVNASS
                     LIANSPQISLSFWVYPRNPVPGFPDFDGLAGFRDNTVADFYVLQYSSTAVEARFRNSA
                     GINFDILSQGLQLNTWQQYALTYDGSFLRLFRNGLITDSVSASGVISSTATSFFMGNL
                     VFSGTNFLLDGQMDEVALWNRALTPQEVLCIYKNKVDATSPGLQLYYDMNIGIAGGNN
                     STILTVPDISNHINGVLQNFSMTGTSSNLVNGIDNATIITDTICSGQTYSFNGQTLTQ
                     AGVYTATLVSSANCDSIVRLTLFSNDTTVNQNQALLTAVQNGATYQWVDCSNNFSPIP
                     GATNQSYTATANGSYAVIITANGCTDTSACHNVTTVGINDYSASNWITFPNPTVNVFN
                     IVTNEIINNSEIIITDISGKQISRMTKDIKYHCAVDVSALPGGSYIITLKSEKKSKNW
                     RLIKI"
     gene            complement(1850..2536)
                     /locus_tag="PKY09_00795"
     CDS             complement(1850..2536)
                     /locus_tag="PKY09_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11415.1"
                     /translation="MEPANDKIAQLIKTHFPLNCKIVEFTTGHFNEIKNYLKKFVDAT
                     ATYYQAPNKRQRIKFYDTGSNQCELQLGDDTLWFHLHPNLFRISPDNPAYRYSYLRDD
                     EYRSRCAVIHVYNFLSDSLAERRSDDYGMLIARIFVNYENHFMVEGRKQLGMLFNDIS
                     NDILTEQRLNDVLEQVILFCLQYDLFAQPFDQVPAMSYREISSGSFGSAVASSKQLGF
                     KLKAEDHDTE"
     gene            complement(2548..4023)
                     /gene="proS"
                     /locus_tag="PKY09_00800"
     CDS             complement(2548..4023)
                     /gene="proS"
                     /locus_tag="PKY09_00800"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNB11416.1"
                     /translation="MAKDFPTREQNYSEWYNQLVIRAGLAENSSVRGCMVIKPYGYAI
                     WERMQQQLDSMFKATGHSNAYFPLFIPKSFFSKEANHVEGFAKECAVVTHYRLKQSDD
                     GKSVVVDPEAKLEEELIVRPTSETIIWNTYRNWIQSYRDLPILLNQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATAEEAVEETRKMLDVYAEFAEQWLAIPVVKGVKSANERFA
                     GALDTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFTTKQGVQDFVWATSWGVSTR
                     LMGALVMAHSDDDGLVLPPKLAPIQVVIVPIYKNDEQLKLVSEIALKIKKALTARGIN
                     VVYDDDEQAKPGWKFAEYEMKGVPVRLAIGPRDVESGTVELARRDTKEKMTVQITDIE
                     LKIENLLEQIQQNIFKKAVDFRDTSTHVADSMDEFIKILDTKGGFVFAHWDGTVETEE
                     KIKEQTKATIRCIPLNNKQEKGKCILTGKPSTQRVLFARAY"
     gene            4177..5817
                     /locus_tag="PKY09_00805"
     CDS             4177..5817
                     /locus_tag="PKY09_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11417.1"
                     /translation="MKSIALSAAAIFTLASCTSSQQASNAYSDDVYYSSKANKPVETV
                     QTQQQVQQPQQDANNQTESNQGSSTSAENRFDYNDAGTTTQDGNTYVTNNYYSDDDYY
                     DYAYSSRIRRFYRPMWNYGYYDSYYTNSYWYDYNPYDYGVSIYCGYNWWAPSFWWAPS
                     FTYFSWGYTPYNYWWSSPYSYYGYGPYHHHGWNNYSYGYWNGYNNGYWNGYYDGYYNG
                     YGYNPYYFNSYDSYSYNNTYYGPRRSTSGSGSSNTVPPRSIADSYNRALVKNGRNSMP
                     VSQSFANAMSLPTRDTKSTEAEVIHPRNNVNTNSPVKDSRNVETAPVRNNETEVIHPR
                     NNANTNSPVKDSRNVETAPVKNSRADQFSTPRNNGDVKDNSNTPVAPPVRNNSKNEVN
                     PEYNSPRNTETVPPRNYESNPRYNNNTPAESPRRNIESNPRYNNNTPAESPRRNIESN
                     PRYNNTPAESPRRNIESNPRYNNNTPAESPRRNIESNPRHNRNSETAPRQEQAPRQER
                     VTPTPRQERSFSAPSQPRQESRPRQESTPSRNNSRNGR"
     gene            5846..7345
                     /locus_tag="PKY09_00810"
     CDS             5846..7345
                     /locus_tag="PKY09_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11418.1"
                     /translation="MDYRFKSMLAACFFLTATAFAQNENDALRYSMQQNFGTTARSLS
                     MGGAFGALGADFSSLSINPAGIAVYRRSEFTFSPVLQFRNNDASYSGTLTTDNRANLG
                     IGNLGFVLAFPREKKTSGWMGTSLGFGYNRINSFSDRFAFEGINKNNSINDYFAEISN
                     GISKANLSGDEMYQFDAGLAYNSYLTNPVADSSLIDQYQGALFNGDLKQKGLITRTGS
                     QGEIDISFAANYDNKVYLGATVGIPYFRYSSTEVYEEYALQNTIKDSLSGKTFKNSKY
                     TQKLSTQGNGVNLKLGVIFKPTDMVRLGLAIHTPTYYEMNDQYSSKIYANFSEGYLEN
                     KSPDGRFSYNLTTPFKTIASVGLVFLDQGLISADYEFLDYSVARFNSNDYNFSDENRD
                     IKNRFEQGHTLKIGAEYRYKVFSFRAGTVMQSSPYEGGITYKEGNRSAVSYTGGIGIK
                     GGNFFLDLGYSFTKTSGNYAPYTLADGSAPSASVATKINRFMMTFGAKF"
     gene            complement(7352..7918)
                     /locus_tag="PKY09_00815"
     CDS             complement(7352..7918)
                     /locus_tag="PKY09_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB11419.1"
                     /translation="MKNGLLIYCVLLTLAVAYLFYKVNSCTSNCAPAGVTTAMNDGDI
                     VFVNVDTLLEHYDYYISLRESLKKKQDSIDVILKNKGKELEGEIVNYQKRGASMTDQQ
                     RGAEEERLGHKQQQLVEYKNHLLDQLTEEEAVMQDSLHKHLVGYLKDMNKQTNFKFVF
                     GYQRGNGILLANDSLDITQSVIEGLNKK"
     gene            8062..8991
                     /locus_tag="PKY09_00820"
     CDS             8062..8991
                     /locus_tag="PKY09_00820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNB11420.1"
                     /translation="MQIIEPVERHLLEQELNNGRFIRNANNGNNKLYVVTAHDSPNVL
                     KEIGRVRELTFRAAGGGTGKAFDLDEFDLIENCYKQLIVWNPADKEIVGGYRFIRCAD
                     AATDPHSQFKLATTELFHFTEKFINDYFPHTIELGRSFVQPQYQPSADNRKGLFSLDN
                     LWDGLGAIAIDNPDIKYFFGKVTMYLRFNRIARDMILAFMQHYFPDTDKMVYPHHPLP
                     SENDVSGFVASLKNLPYKEGHHILNKKVRELGENIPPLFNSYMNLSATMKTFGTALNH
                     EFGDVEETGILISIDDIYESKKARHVNTYHPTL"
     gene            complement(8988..9959)
                     /locus_tag="PKY09_00825"
     CDS             complement(8988..9959)
                     /locus_tag="PKY09_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11421.1"
                     /translation="MRNTFYFLVFALIIVTSCHNDKSSDNPPCPAMEMHISGDTTVVA
                     GDSIGLVATLFTGATYSWTGPNGFVANTFNPVISESSSLSEGYYYASADINGLCKGNV
                     DSFYVTVICDRPEDVLANYITTIQTNDTIHLFAHSITDSVEYIWTGPAGFTSTQPNPI
                     IINAIPGNEGVYTVYAHKDHCYSKPDSVVVSFNQCNPSSNTFANSLDSNYFLYSYGSA
                     NLYPNTRSITGTGLNGTSQLRATFYGNNPPTGNYTINPAHCPASGSGALAPGEVCVEF
                     SNASGTYQAASGTVKLKALNTFDFCDAPFRLQFTTTTLFNGSGKVQF"
     gene            10031..10645
                     /gene="yihA"
                     /locus_tag="PKY09_00830"
     CDS             10031..10645
                     /gene="yihA"
                     /locus_tag="PKY09_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNB11422.1"
                     /translation="MEISSAEFVQSNTDVKKCPADSLPEYAFIGRSNVGKSSLINMLC
                     NKKGLAKTSSTPGKTQLINHFIINKQWYLVDLPGYGFAKTARTEREKWDGMIRNYMIY
                     RKNLMSVFVLIDIRIAPQQKDLDFINWLGEKNIPFSMVFTKADKQTRAKNDFSVKQFC
                     EQLYKTWATLPPYFVTSAETKSGRQEVLDYIDSINQSFKLNGTQ"
     gene            complement(10657..11037)
                     /locus_tag="PKY09_00835"
     CDS             complement(10657..11037)
                     /locus_tag="PKY09_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HNB11423.1"
                     /translation="MKYLFLSFLLGMFSLSVQAQVASKLASKPDSSKALMSVEATCGE
                     CKFDLPGNDCDLAVRINGKAMYVDGVSIFEFGHPHDDNGFCVAVRKAEVQGEVVNNRF
                     KATYFKLLPVANATENTNRQDAGK"
     gene            11539..14607
                     /locus_tag="PKY09_00840"
     CDS             11539..14607
                     /locus_tag="PKY09_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11424.1"
                     /translation="MKNQSKIIIVIAVLFISTTNIKAQNNAIIDINAQLRGLFSPLAK
                     PNPSLEFLYDMSAKVSDSVFYTTFCKDTLETPMWQSIYDEMYHTAYDTSWLPIPDDFF
                     LSGNNFYSDTIPMGILNFGFYKFLDDAMTTNIYFNFDTVNDILTDKYPRPGFPYIDSN
                     LFVAAPMIASAQFSNPVYRVDPQFILFDALNAGIYLHGEGILKIDFGDGNGWIQFDPT
                     IITNWHVQYASAGEKLIQTAIFDHGNDMLFYSLSRIVIVSGTASIPPDQYIESEGLIV
                     GYYGGCNSQGISAKTVIYLSGIDILDFIPSKNRTIATIYSEMIKQDNIVQLKNQGYGF
                     IVVDYANSRIDVRFNALYFVNLLENLKCLLQDINQQQFVVMGESMGGLVARFALTYME
                     SEYYMQQNTSPFFVDQFDLNNIPYLALHPNIYNMPSQWCRTEKMHNTRLFITLDTPHQ
                     GANIPMSMQYAYKTGIDVLSRFISFDLRLLTNAFNLFLDGQAAQQMLIYHIDTKSGSP
                     FYYNYDSKSDKYFFFDQLNALENYPQYAKVIAMSQGSMSGEMQRNNYTGSDRNPGDRL
                     LDFSADLYARVLWLKVPIFGGDLECKTNPNGNGHIFLAQAGWYGIRIKLKWFGIKVTI
                     GYNSLLYVNEYANLLPYCVNPGGIFGDPDFISTTPSQGGFNLSNNYWLLNFFHCSHTV
                     NGNGCVDFDSHLGLNGFLSTNFDYSLCSDGLQFGFVPVQSALDYGVLGGIPLNFDIVS
                     ENINTKLSNTPTDMMIGAYNNFTATYPNLSHVGFRNNYVYNLTDQPNNGPHFCTYYSC
                     IGNNENVERSLLNSEIGDENLFLENVTLHWETEYQPEYDMFVNVRNPRYEYPSQPYSN
                     LNYMFLGVYSKEDPYIIDNSGYATFYFDGANTPTGTGFVYDPPYTGNYNHIDQPLSVC
                     CENFMERKANTTKKKPNLVSSLEIFPNPNDGQQVVLNFRFKEYGQVNFSLYNSTGILI
                     GSEILKTKDASTPTTSVLNLSNRIFASGLYYIQLISDKGETLSQKLLINK"
     gene            14661..15329
                     /locus_tag="PKY09_00845"
     CDS             14661..15329
                     /locus_tag="PKY09_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11425.1"
                     /translation="MKKYIINIKFLALSSLVLIFQSCKKDTDSPGDGPTYTITGRLFE
                     DCNMQPVANQKIDLFQEYSTGLDGNLNGGILAQTTTDANGNFKFDFKDMDGFTESIRI
                     PAGQGYTKLFQNIPQNQSLFDLRVYKSPTTNIKVNLIVNNSYTNLDTLRLTDFNNLST
                     PYKISGPFTSSLLYESVDFPILNFGYPQNILNFGYKLNNNSEVMSTPTITTCGITNIE
                     VVIN"
     gene            complement(15592..15972)
                     /locus_tag="PKY09_00850"
     CDS             complement(15592..15972)
                     /locus_tag="PKY09_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HNB11426.1"
                     /translation="MKYLFLSFLLGMFSLSVQAQVASKLASKPDSSKALMSVEATCGE
                     CKFDLPGNDCDLAVRINGKAMYVDGVSIFEFGHPHDDNGFCVAVRKAEVQGEVVNNRF
                     KATYFKLLPVANATENTNRQDAGK"
     gene            16293..19757
                     /gene="ileS"
                     /locus_tag="PKY09_00855"
     CDS             16293..19757
                     /gene="ileS"
                     /locus_tag="PKY09_00855"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNB11427.1"
                     /translation="MNHKPYPEFKQLDLAGIAKEILTKWDAEKTFEQSITLREGSGAF
                     TFYEGPPSANGLPGIHHVMGRAIKDIFCRFKTLQGYQVKRKGGWDTHGLPIELSVEKT
                     LGITKEDIGKKISVEEYNKACRKEVMKYKEVWDDLTRKMGYWVDLDKPYITFENGYIE
                     TCWYLLKELYKKNLLYKGYTIQPYSPAAGTGLSSHELNQPGTYKMVKDTTIVAQFKIK
                     KESLPENLASQLSDIKSDFYFLAWTTTPWTLPSNSALAVGEKINYVWVQTFNQYTNKP
                     IVVVLAKDLFAKYFSEKNTALSLDDFKPGDKQIPFKVVAECKGSALTGIVYEQLLPYV
                     EPDGKAFVAVAGDFVSTEDGTGIVHLAPTFGADDFRTAKQQGIEAILVKDETGNAMPL
                     VDKRGRFVSEVTDFSGEFVKEEYLSEEEKEQERQKQNRDKYLSVDERIAIKLKQENKA
                     FKVEKYEHNYPHCWRTDKPVLYYPLDSWFIRTTACREQMIALNKTINWKPESTGTGRF
                     GNWLENLVDWNLSRSRYWGIPLPIWRSEDEKEEKCIGSIGELRKEIEKAIAAGVMDAK
                     HLEHVDASKSDFDLHRPYVDNIVLISDTGQKMFRETDLIDVWFDSGAMPYAQWQLDQD
                     KLSKGLPMPFNEGFNTGYPADFIAEGVDQTRGWFFTLHAIAVMLFDSVAFKNVVSNGL
                     VLDKNGNKMSKRLGNAVDPFETIGKYGPDATRWYMISNAQPWDNLKFNVDGIAEVQRK
                     FFGTLYNTYSFFALYANIDGFVPDEKNKIPLHDRPELDRWILSKLNNLVQRVTESLDD
                     YEPTFAARLIEEFVDEHLSNWYVRLSRRRFWRSEMNTDKQSAYETLFECLKVTAQLMS
                     PVAPFFSDWLYRNLTDSLRSNAIAAKTPLQYSSVHLTYLTKAETNMIDAALEERMDFA
                     QRISSMVLALRKKVNIRVRQPLQKILIPVDSTQQQKAIEQVEKLILTEVNVKEMEFIT
                     DTSGLLVKKIKANFKVLGKKLGALMKDAAAAIAQMTQDDIRILENKGAFDLRINENIV
                     VISVEDVEISSEDIPGWQVNSNGGLTVALDISISPQLRDEGIARELINRVQNLRKDKG
                     FEVTDKIMVKLKNQSSITTAIESNLDYICAEILASSFTWVETLATPVADEIEVEEGVK
                     TLIEIERI"
     gene            19760..20746
                     /locus_tag="PKY09_00860"
     CDS             19760..20746
                     /locus_tag="PKY09_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNB11428.1"
                     /translation="MASKAKKKPVAKKATAKKATAKKATPKNKKAAVKKVVKVVKKAA
                     AKSKPSTKKAVAKKVVVKGKPVKAKPAPKKAAAKSKATKSKVVAKKVVKAKPVAKAKV
                     KAVKPAKVAKVKTAKVVKPVVASKPQKEDKNKSATSMIKHVEKPRESSRRRSNPPIVK
                     PAAPPRTTPLREDPVLPDAPVASILKKPVAMLDNRPTKLENSPDKTRYSDAELNEFRE
                     LIIQKLTDAKVELNNLQAFLNNSNEHGTDDTASSFKMLEDGSDTLAKEEAGQLAARQR
                     KFIEQLENALVRIENKTYGLCRVTGKLIPKERLRAVPHTTQSIEAKVQQYRD"
     gene            20818..21483
                     /locus_tag="PKY09_00865"
     CDS             20818..21483
                     /locus_tag="PKY09_00865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNB11429.1"
                     /translation="MKKYITLVAVILIIDQCLKFWIKTNMYLGQEYHIAGNWFIIHFT
                     ENNGMAFGMELFGKKFLSLFRIGVMFALAWYVWHLHKTKAHHIYTTAMALIFAGAVGN
                     IIDSIFYGRLFSSSEFGVATFMPDAGGYAGWLHGKVVDMFYFPIIQTHYPQWFPFWGG
                     EEFIFFRPVFNIADSAITAGVLLIILFNRTIFKHIETTPETEQTTAAIPSEELNASES
                     HNS"
     gene            complement(21542..24061)
                     /locus_tag="PKY09_00870"
     CDS             complement(21542..24061)
                     /locus_tag="PKY09_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11430.1"
                     /translation="MRLLSTAVILLGSLCSQAQISITNNEMPSAGDTARFTKAVVNPL
                     INFSATGANHTWSFGNLRNNGQDLKSYQSVSSTGFIYSLFFSNLPFNPNRANVTEPGQ
                     ALPSNPLLTITDPFNFYYRSSNVYKQVGFGAEIANIPMPVAFSQHDIIYNLPLNFNDA
                     DTSQSAWNIGLPGLGYYGLSQTRINHVDGWGMLTTPHGTFNTLRVKTQLYIKDTISVD
                     TLNLHFALDRPKTTQYKWLANNEIIPVLQITTTEILGLEVVTEILYRDDYNTVSPGSL
                     NAAYCAGSSISIPYTATGSFNGSGFLQSANSFTAQLSDATGSFANAVNIGNVTSRVSG
                     NISAVIPANTPAGNGYRIRIVSSSPALTGGDNGFDLRIENSAVASIVNQGLSAFCSGN
                     SALLQSVNSDSGYTYQWQLNGTDISGATTDSYPASATGNYVLNITNSCGTVASNAVSI
                     NVMPLPTVLVTASGALSVCQGNAVTLSTLFDSTYSYQWQLNGVDISGATTENYTAANG
                     GDYTVVVSNSCGNVTSSVQTVSVQSAPIAGSLTAASSTLCHGDSVLISYSLQSAGITW
                     LYNGNMLPGISGNNHFANTAGVYNIVESNLCGSDTSAAVILTADSLPIAVITASGALS
                     FCAGGDVVLTAPSGNNYTWQWNLNGTPLATSQLITATQSGNYEVEVTNVCGSATSTLV
                     TVTAYPLPAVPTIVLSNDSLLATTSAGTYQWFYNGNAITGATNDFFVPSQNGDYTLLI
                     TDANGCSAMSAVFGMTTVGLQTMFKQAHNVFPNPFNDYLTIELPKTENAVIMTIEGKQ
                     LWSGILHQGRNVMNMQQFVSGIYILKLNNENGNTNYKLVKR"
     gene            complement(24379..26013)
                     /locus_tag="PKY09_00875"
     CDS             complement(24379..26013)
                     /locus_tag="PKY09_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNB11431.1"
                     /translation="MNKKHPKALPYLFLSEMWERFGFYLMLGIFFLYMTDTQRGGMAM
                     DRKEASDIFGTFIALVYLTPFVGGLIADRVLGYRVSITLGGILMGIGYCGLAIPGMTA
                     FYVSLLLIIIGNGFFKPNISTILGNVYNDPEYKPLKDSGYNIFYMGINIGAFICNFFG
                     SYLRNNFSWGAAFAAAGIGMFVGVIIFWIGNKHYAHADVRKPVKPEDMPVSKILGITL
                     LPALIAGYIGWIIPDNIFGSDSTDAFILGALPVAGYYVNILLRSDKEDRRPLAALLAV
                     FTVAIVFWAIFKQNGTALTTWAESYTNREVPQRVEPVAKMLGVTQTVTYAKDSVPLTD
                     NQFRVARDENKKPIKTWDYPLYYKNIPVDKLPKEGESLTLISTELGQSINPFWVVVLT
                     PLIVAFWAFLKRKNSEPSTTWKIFYGFLITSLSTLVMVAATYACHNGLEKSSMWWLIA
                     SYGVITVGELCLSPMALSLVSKLSPPRLTALMMGGWFLSTSIGNKLSGVLASMWDEYE
                     HKANFFLINFVLVMITTVIILLMLKWLNKIVKEHGA"
     gene            complement(26075..26704)
                     /gene="tmk"
                     /locus_tag="PKY09_00880"
     CDS             complement(26075..26704)
                     /gene="tmk"
                     /locus_tag="PKY09_00880"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNB11432.1"
                     /translation="MKKENLFIAIEGIDGSGKSTQVQLLKTNLEQVGHKVYTTHEPTS
                     FEAGKLIRSIFNHKVNADQHTIAALFVADRLQHLLDKEHGILAMLEKGYTVITDRYYF
                     SSYAYHSAYVEMDWVMSINAKCAALLRPDRNIFVDVDPETAFRRIVSTRTSTELYETN
                     ENLRKVYSNYLKAFELLKESENIVFVNGKNEVQQVASDIFKSVKNLLHT"
     gene            complement(26729..27715)
                     /locus_tag="PKY09_00885"
     CDS             complement(26729..27715)
                     /locus_tag="PKY09_00885"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNB11433.1"
                     /translation="MKQLIEKFPAQMREAMTIGETFKATSWPHEIKNVLVTGLGGSGI
                     GGTIVSQLCEKEMPIPFLVNKDYFMPSFVNKHSLVVVSSYSGNTEETLQAMDNAIKQG
                     AKVVCVSSGGKTVELAKQHGLDYILIPGGMPPRSCYGYSSTQLFYILHAFKLIGDTFK
                     SSLKKAIELIESESQHIKTASHKLASDLIGKIPVIYSASNNEGVVIRFRQQVQENGKM
                     LCWHHVFPELTHNELVGWTQPHNDIAVVLFRDKDDFFRTSKRMDICKEIFGRYTPHIY
                     EVWSKGGSPIEKAIYLIHFGDWTSWYLSEIRNIDCTEVKVIDYLKSEMAKLN"
     gene            complement(27797..29578)
                     /locus_tag="PKY09_00890"
     CDS             complement(27797..29578)
                     /locus_tag="PKY09_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNB11434.1"
                     /translation="MKKYLFIACALFIISCSENRKQSIKDTSVLLKEITDDYYQERMR
                     YFPLEATANAYNRYNDLLPIDISDAYIDTLRQFYHSYSEKLLTINKPELTGQDLISYE
                     VLQYILNTEEEGLKFPSNLMPVNQFWGMHLTFSQLGSGTSSQPFKTVKDYDNFLKRIT
                     AFVAYQDTSIGNMKRGMLQGIVPARILIERTIPQYKSLIASKVEESVFYGPIKNMPDS
                     FSDTDKERLTIAYSKAILQELNPSFKKMSVFLEKEYLPACLPVAGISNIPGGKEYYNW
                     LANKWTTTTMTPDEIYNVGLKEVERLHTEMDKVKAETGFKGTLKEFFHFIHTDPQFFP
                     FKNDSDVIAAYLAIEDRMKPQLKKLFNHVPKAAFEVRQTEAYRAASASAEYNTAAPDG
                     SRPGVFYVPILDASKYNVVGMEDLFLHEAIPGHHYQISLQQENTSLPEFRRFGWFGAY
                     GEGWALYTESLGKELGLYTDPYQYFGCLSEEMHRAIRLVVDVGIHLKGWTREKAIQYS
                     LDNEMESEADITAEIERYMAIPGQALSYKIGQLKIIELRQKAEEQLKDKFSLQQFHDQ
                     LLSYGCLPLSVLEKSINQWIKEKKRQG"
     gene            complement(29655..31124)
                     /locus_tag="PKY09_00895"
     CDS             complement(29655..31124)
                     /locus_tag="PKY09_00895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNB11435.1"
                     /translation="MTIDWEKLGHQFLYDKANPLLFNSSFFLFYLLLFLFVFRILQRT
                     GTLRIVAVTLFSLYFFYKASGVYVLLVLVAAIVDFNLSNLIHNATTDFRRKALLVFSV
                     VINLGLLAYFKYTNLLIDLYNAVLTGNVQPADVYLPIGISFYTFENLSYTIDVYRRQF
                     VPVKRFIDYLFFLSFFPKLMMGPIVRAADFIPQIRKEITLTAHDVSKGLFLILTGLFK
                     KVVISDFIYLNLVQYVFDDPLRHTGMECLFAVYGYAMVIYCDFSGYSDMAIGIAKWIG
                     IDININFLAPYQSASITEFWRRWHISLSSWLRDYLYIPLGGNRRGEVRTYLNLFITML
                     LGGLWHGASLNFIFWGALHGVALAFHKLWKAAYPSHFSGRLTRIAGIFITFHFVCLCW
                     IFFKCTTFTDSFNMIHQIVFNFYAEGWTLFSTNYSEVLLVMLLGFVLHFLPIRWNEII
                     INYSEKWHWLVKASVATAVIIIVIVLKQADPVMPVYLQF"
     gene            complement(31121..32362)
                     /locus_tag="PKY09_00900"
     CDS             complement(31121..32362)
                     /locus_tag="PKY09_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNB11436.1"
                     /translation="MKRIILILSLLIYCTRCHAQSESDTIMPVLQYEKFVAYKMSLLK
                     FLNPDAAHIEHQEALKNFYSKLLQSKTAGKKISIVHIGDSHLQAGYFSGMVRRGLQQR
                     YGNAGRGVVFPYRLARSNGPHDYVSFSQNNWKGNRNVVTQDSLSTGITGFSVTTNDSN
                     PSVGISLRNNEGIDYRFNKVQVIYKPDSAVMTASDSSEIMETVNPIKLSAEINEFRFS
                     NYLTNCKIKINSDTAFTLFGLNLLNDSSGVIYHTIGVNGAEYNSFLRQPLFFEQVKTL
                     DADLYILSFGTNEAFAKNFDGIIFIQTVDSFITKLRNVSPSASILITSPPESCKRIRR
                     GVYVPNPNVIAIRNLLQKYCIENKIAFFDLYEYSGGKGSMLRFAKSKMSDARRIHLNR
                     SGYEMQGLMLLQALLKPLTEQ"
     gene            complement(32359..33756)
                     /locus_tag="PKY09_00905"
     CDS             complement(32359..33756)
                     /locus_tag="PKY09_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNB11437.1"
                     /translation="MKNFRNNEPVKILFFLVIILTVISLLPKKFTGSKTINLYSDIIQ
                     KENKKEKSQDLAIVNDSVFFKNDTVKQIVTDTVRATAIKINYKVGELDLTKFFEALHN
                     HANKNEKIRIAWFGDSMIEGDLVTQDFRKLIQKKFGGSGVGFVPVTSPVAQFRQTIKQ
                     TFSDDWEVYSFMKKPKQNYKLGIGGYSFVSSDGSWVHYNASRFYPIYSEVKMICRQCP
                     SASFILNDDTAHIFPTDSINGFKEIELVKDKPIKEVKLTFEKPGCEVHGLYFDNGNGV
                     YVDNFSFRGNSGIPLSNIPFGELQTLATKENYKLIILSYGLNVVAHDVKDYSWYASSF
                     QKTLNYIKEAFPEASILIMSVGDKSYRAEDGYETEPDIPIFVELQKRIAQRSGVAFYN
                     LYEAMGGYNSMVGWAEGNPRLANLDYTHPNFAGGRKIAGMLFDDIVKSYDRYSKTVEV
                     MDVKKDTMINPYVKQ"
     gene            complement(33772..34248)
                     /locus_tag="PKY09_00910"
     CDS             complement(33772..34248)
                     /locus_tag="PKY09_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNB11438.1"
                     /translation="MKSSFRLVRQYTFLFLIALIFNSDNLFAFSDSAKVVVNKHRKEK
                     YYKQPRVNVDDVKSYMDSIGVLNSDIVLKQALLETGHFKAKLLMDKNNLFAFRFTKRY
                     MSFANWQASVDYYKKWQDKFYTNQSENYYDFLKRIRYARSPHYIKVLKQVKPRTAQ"
     gene            complement(34387..36207)
                     /locus_tag="PKY09_00915"
     CDS             complement(34387..36207)
                     /locus_tag="PKY09_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB11439.1"
                     /translation="MEVKQTADVAVEHHDDHTHEHDHHETFLTKYIFSQDHKTIAKQY
                     LITAIIMAFFGMYMSLIFRLQLGFPETKFAFLESLIGKWGKGGRLDPGFYMALVTIHG
                     TIMVFFVLTGGLSGTFSNLLIPLQVGARDMASGFLNMLSFWFFFLSCAVMTYSVYIET
                     GPAAGGWTVYPPLSALPQAMPGSGLGMTLWLVSMVFFIIQSLLGGINYICTVINLRTK
                     GMSMTRLPLTVWAFFITAILGVLTFPVLFAAALLLVFDRSFGTSFYLSDIFIGGVALP
                     QSGGSPVLYEHLFWFLGHPEVYIIILPAFGLTSEVLSTMGRKPIFGYKAMVGSLMAIA
                     FLSCIVWGHHMFVTGMNPFLGAVFTFTTLLVAIPSAVKVFNYLATLWRAKIQLTPAMC
                     FAIGLVSLFISGGLTGIILGDSALDINVHDTYFVVAHFHVVMGASAIFGMLAGVYHWF
                     PRMFGRMMNKTLGYVHFWLTLIAVYGVFFPMHFIGLGGVPRRYYSNSAYEGFDVFLNI
                     NTLITVFAFLAAIAQVIFLFNFVYSIFKGKKAPQNPWHSNTLEWTSPAGPGIHGNWNG
                     PIPTVYRWPYDYSKPGADDDYIPQTVPYSQTKNSNLPGEE"
     gene            complement(36259..37284)
                     /locus_tag="PKY09_00920"
     CDS             complement(36259..37284)
                     /locus_tag="PKY09_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNB11440.1"
                     /translation="MTLLITITILLGIIVLARLMTIVQLSSELTGSSDSQEEQVRHNN
                     SNGFGFIVFMVLGLGLFIYMTIDYSKHLLPVSASEHGVGLDKLLNINWAIIGVVFFIT
                     QIALFYFSYKYRDNKKHEAYFYPENYKLELIWTIVPTIVLTTLIITGLKQWNGIFSEK
                     ENSMNVQVYGYQFAWITRYSGPDNILGKSNYKLITDDNPLGLDASDPAVKDDIYTTGN
                     EMHIPLGQELKFQFNARDVIHSAYFPHFRAQMNLVPGMNTFFSFKPTITTAEMRKITG
                     NDKFDYVLLCNKICGVAHYTMKMKVVVDTPADFKAWLKSQKKASEVKAAPPVSTLNST
                     ANGSNSI"
     gene            complement(37325..38527)
                     /locus_tag="PKY09_00925"
     CDS             complement(37325..38527)
                     /locus_tag="PKY09_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HNB11441.1"
                     /translation="MQDNLTYTFSSKLKTLSFILMAIGAISIGISFATNPDQTWANLM
                     HNNFYFLTIALGGLFFYAVQYAAEVGWSVYIIRIPLAMSQYLWISGVIMLILFFAAGH
                     HIWHWTHHELVMETLPDGKPNPDYDEILAGKSAYLNVPFFLIRMVAYFVIWYGFAVLL
                     RKHAMKEDLTGAIEHHNRSRKLSIIFLVLFAVTSSTSAWDFVMSIDSHWFSTLYGWYT
                     FSGIFVSSLGVIALIMVYLRSQGYLHHLNENHFHDVGKFIFAFSIFWTYLWFAQFMLI
                     WYANLPEEVTYFMERQDYYKTLFVTNFLVNFILPFVVLMTRDAKRKLRLLAILGSVIF
                     IGHWIDTYLMVVPGIMHEKGTIGVLEIGTTIGFIGMFIFTVFTALTKLSLLPQKHPML
                     QESEHFIL"
     gene            complement(38556..39161)
                     /locus_tag="PKY09_00930"
     CDS             complement(38556..39161)
                     /locus_tag="PKY09_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB11442.1"
                     /translation="MKKTYQYLSVIVSSMLLLGACGDKQSPGLEYMPDMYRTPAVVAY
                     SPSHITKDSVSALAPVAGTVARGQYVDFIYPNTNEGYEAAGLELKNPFQPTVENMERG
                     KTEFDTYCIHCHGEKGDGKGWLKIKGDPFPVPSYTSAAIKDLPDGKMFFSITYGKNLM
                     GSHAAQVSADDRWKIILYINHLQGDEPGAVVADSSATAQNK"
     gene            complement(39175..39687)
                     /locus_tag="PKY09_00935"
     CDS             complement(39175..39687)
                     /locus_tag="PKY09_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNB11443.1"
                     /translation="MTKQLHGIFNDDEVLLNAIKELKSKGIKCTNAYSPFPVHGLDGA
                     LGLKRTRISIASFLYGATGTCLALLMMWYMNIWDWPMDIGGKPSWNLYKNIPAFIPIT
                     FEFTVLCAAHGMVITFYLRSRLLPGVTPNVIHDRMHDDHFVLTVEGKVSDETAIRESL
                     KQVGAVEIRS"
     gene            complement(39680..41065)
                     /gene="nrfD"
                     /locus_tag="PKY09_00940"
     CDS             complement(39680..41065)
                     /gene="nrfD"
                     /locus_tag="PKY09_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNB11444.1"
                     /translation="MSNVESPLRPTLIEGDKTLHDITEDVCRPIESKPNKYWWMVFSV
                     ACAALLWWIICVAYTVGTGLGVWGLNKTVGWAWDITNFVWWVGIGHAGTLISAILLLF
                     RQKWRLSINRSAEAMTIFAVICAASFIVAHMGRPWVAYWPLPFANQFGSLWVNFNSPL
                     VWDVFAISTYFSVSLVFWYTGLIPDFAAVRDRAKTKARKFWYSALSMGWVGSAKNWQR
                     FEEVSLVLAGISTPLVLSVHSVVSMDFATALVPGWHTTIFPPYFVAGAIFSGFAMVQT
                     LLIITRKVLNLEDYITIYHIDMMNRIILITGSIVGCAYLTEFFIAWYSGVEFEQYAFI
                     NRATGPYWWAYWSMMTCNVISPQLMWSSKLRRNLFVTFFLSIIVNIGMWFERFVIIVT
                     SLHRDFVPSSWTMFHPTWVEIGLFIGSIGLFFVPFLLFTRYFPVIAMSELKTILKSSG
                     SQSKKGVQHHD"
     gene            complement(41103..44204)
                     /locus_tag="PKY09_00945"
     CDS             complement(41103..44204)
                     /locus_tag="PKY09_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNB11445.1"
                     /translation="MEKRYWKGVEELRNDAEFVRLKNNEFFEEIPVDEVLGKKAESKD
                     ATPRRDFLKFLGFGVAAASMAACEAPVRKTIPYLISPENLVPGIPNYYASTFFDGHDY
                     ASIVVKTREGRPIKVDGNELSPLTKGGANARVQASVLDLYDSERIKGPIAKGKAASWD
                     TVNKEIKDKLASANTKQAKIRIVSSTIISPTTKKAIESFTTKYPTAKLVTYDAMSQYG
                     IIKAHQLAFGKAALPNYSFDKADVIVSFGADFLNNWIAPIEYSRQYAQTRKLDDGKKT
                     MSKHIQFEAALSLTGSNADNRYKLKASQTGNALLSLYNAVASKAGAATVSAGKTDADA
                     AINSTANDLWNAKGKSLVVCGSNNANDQLIVAAINNMLGNYGATISIARHSNVRQGND
                     EAMAELLREMNAGEVGVVIFYNANPLYNYPDSTAFTSALNKVEVKISLSDRNDETAVK
                     CDYICPDSHYLESWNDAEPVRNMFSLVQPTIQKLFDTKQSQELFLLWSEAAVTDYHEF
                     LMQNWRQGFLATATGMASIPAWEKVLQEGVWNGEVTESEPAFVGNVNDAASAIAARKS
                     GGLDIVVYEKAGIGNGRHANNPWLQELPDPVSKICWDNYFAIAPSMAKEKGLVQGNII
                     EVKSGNVTLKGPVLLQPGQAKDTIAVAAGYGRTNSGKAGNKVGMNAYPLLTFSEGTFN
                     FTNSGASLNKTVEADYTLATTQSHHTMMGRAIVKETTLEEYIKDPKAGNEEEKFHSYK
                     GKVSAKELDLWATKDHPGFDRPINAWGMSIDLNACIGCGACVVACTAENNVPVVGKEE
                     IQRSREMHWIRIDRYYTSDMDKEKAHEQGIGKLSMYGYMEEPAENPEVVFQPVMCQHC
                     NHAPCETVCPVAATTHSEDGLNMMAYNRCVGTRYCANNCPYKVRRFNWFKYSDNPQFD
                     YNMNNDYGKMVLNPDVIVRSRGVMEKCSMCVQRIQYGKLEAKKAGHPVKDGAINTACA
                     QTCPTNAITFGDYNDKSSRLVKQSSSERMYHLLEELNVQPSINYLTKVRNTTSKTNNS
                     HKA"
     gene            complement(44275..45459)
                     /locus_tag="PKY09_00950"
     CDS             complement(44275..45459)
                     /locus_tag="PKY09_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB11446.1"
                     /translation="MLLILGISLGVKAQDGKALFDANCKSCHAMNDVVIGPALKNVHK
                     KFSAEWLHKWIKNSQALVKAGDKDAVAVFNEFNKMVMPNQNLSDAEIDAVLAYIGKES
                     EQAPVAAAPTSPAPGTVTPEKDNSTLYWVIAIFLILLALAATLNKIKKGLEYAVREKE
                     GIPHPPHLEPKEARSLWISKNKKLIAVVLLLLVVFGSVKGWYALKDIGVYTNYEPEQP
                     IHFSHKLHAGDNKIACLYCHSGADKSRHANIPSASTCMNCHKYVKEGPVYGDKEIKKI
                     YAALDWDGSKYGTNQKPIQWIRVHSLPALAYFNHAQHVTVGKIECQTCHGPVQEMDVV
                     KQFSPLTMGWCINCHRETEVKMDGNGYYDEVHKTLAAKYGKDAKLTVDKIGGLDCARC
                     HY"
     gene            45741..46130
                     /locus_tag="PKY09_00955"
     CDS             45741..46130
                     /locus_tag="PKY09_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNB11447.1"
                     /translation="MIKIVKCTCLIICCFLFQLQTKAQTSEDSLTHVLIKKHIAYNIA
                     KPYSQGFRIQLYFGSQRDKAYELRTEFMKLYPQTAAYVLYQQPNFKLRVGDFRTRLDA
                     LKNLNELQAYYPSAFIVKDDIKLKVDE"
     gene            complement(46180..46860)
                     /locus_tag="PKY09_00960"
     CDS             complement(46180..46860)
                     /locus_tag="PKY09_00960"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNB11448.1"
                     /translation="MKKLFTTLAILSLAVIAQAAHPFQSALAITSVDGSLLTVSIDRA
                     PFSNVSTNIYSDRLRAGNHYVKVMRLTGHPHRPVYRTVFAGYLFIPEASEVMAEVNRS
                     RFRITGSRPFDYAPQVYEDPHYYPQHLRCEVDDYEFDQMIQSLGNYNFESTRMTVAKQ
                     LINNNYFSSRQVARLMQQMTFESSKLELAKYAYSKTVDKSNYFIVNDQFSFESSITAL
                     SDYIALRN"
     gene            complement(46998..47618)
                     /locus_tag="PKY09_00965"
     CDS             complement(46998..47618)
                     /locus_tag="PKY09_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11449.1"
                     /translation="MLNRFFSVITYLTHPLIIPTYLLLILFNSDTYLSYTLSPALQRM
                     ILAVVFVLTCLMPFLSSVLLLKRQGVQGLLMEDRQSRTIPYFFTLLYYVACYYFLLKL
                     PVSSVIKILILAAACCIALAFIINFFWKISIHLIGLGGMAGAFYVLTVLMGNEFLLPF
                     VLTLLLAGLTATARLMHNAHTHAQLYAGFIVGFVCEYFFIMWSLSV"
     gene            complement(47621..49147)
                     /gene="rpoN"
                     /locus_tag="PKY09_00970"
     CDS             complement(47621..49147)
                     /gene="rpoN"
                     /locus_tag="PKY09_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNB11450.1"
                     /translation="MLKQSLSQKLLQKLSPQQIQLMKLLQLPTIALEQRIKEEMEVNP
                     ALEEGAPEDVDEDTQQDEDEVPELEEGVEVNESEENKKDEFDNTADDDIRERTDDDFD
                     VDDYITDEDIPYYKTSANNTSPDDERTEVPYGSSVTFQETLLAQLGMVALNDKQMQMA
                     KHLIGSLDEDGYLRRDLNSIVNDLAFTQNLDTNADELLLVLQVIQTFDPPGVGARNLQ
                     ECLLLQLQRKSSSKPVNRAIEIIKNYMDEFSKKHFEKIARLMNIPDEYLKEAMNEILK
                     LNPRPGGDISAETKSVQHIVPDFIITNNVGQLELTLNSRNAPDLRINREYNEMMEGYA
                     KSKKQDRSQKEAILYLKQKIDSAKWFIDAIRQRQATLLHTMQAIMNYQKEYFLEGDER
                     LLKPMILKDIADVVGLDISTVSRVANSKYVQTPFGTFLIKTFFSEGMATDSGEEVSTR
                     EVKKILSDAIEGEDKKKPLPDDRLAKILKDKGYNVARRTIAKYREQLNIPVARLRKEF
                     "
     gene            49353..51200
                     /locus_tag="PKY09_00975"
     CDS             49353..51200
                     /locus_tag="PKY09_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNB11451.1"
                     /translation="MTEVNNDFHADENQQAGETQSENESLAQPLQEEHLAALPEEHLE
                     TLPEQEENYNHLTKEELVKVAEGFAEGDIAVVKSKINALKNAFEQIQSSEKEQALSAF
                     LEEGGVKDDFKYSYTQADDRFQSALKKYNKRKAEYAEQQEKLRENNFNIKQEILVAMK
                     NLIQNEENMQRAFDQFHELQAKWRATGPVPSGQLNDLWMTYKLYTDKFYENLKINREL
                     QELDFKKNLEAKIELCEKAEDLINEPSLNSALQTLHSLQNKWRETGHVGREKSNEIWE
                     RFKAASDKVYERRKEYFEQLKERYAENLAAKNQLITKTEELLKDEPLKASEWMEKSKM
                     LVEVQNEWKKVGFADKKSNDEVWAKFRGLCDGFFVRKNDFFTSLKKEHTANLQLKNEL
                     CIQAEALIENTDWKKTTEELKRLQAEWKKTGFAGERNNQKIWERFRKACDSFFERKNA
                     HYTSLESDYKENFDKKNALIEKAEQFTLGENIDESIEQLKDFQRQWSEIGMVPLEHKD
                     TIYAKFKAIIDKHFQSIRQHNKERFTSGHKQSFRTGKPGNEKNDLKHKMNQLTGEINT
                     WENNLGFFARSKNADAVRKEFEDKINHAKEEIKRIKKQLDEISQAPKEQ"
     gene            complement(51225..51788)
                     /gene="frr"
                     /locus_tag="PKY09_00980"
     CDS             complement(51225..51788)
                     /gene="frr"
                     /locus_tag="PKY09_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNB11452.1"
                     /translation="MNDIAKKIIDTMQSHMDKSISHLETELAKIRAGKANPQMLESIH
                     VDYYGSQTPLHQVANVNTPDARTIVIQPWEKGMLGPIEKAIQIANLGFNPQNDGSIIR
                     IAVPPLTEERRKDLVKRCKAEGENAKISLRSIRKEANDAIKKELKKGITEDEAREAET
                     KIQQTTDGFISKVDKHLETKEKEVMTV"
     gene            51880..52188
                     /locus_tag="PKY09_00985"
     CDS             51880..52188
                     /locus_tag="PKY09_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNB11453.1"
                     /translation="MRFQKHIFVCTNQRKEGERKCCGEVHGLALVAAFKKKIKELQLP
                     VPIRAQRSGCLDACDYGPSVVVYPDGIFYGNVQLHDVDEIVEQHIKNNKPVERLIINF
                     "
     gene            complement(52218..52571)
                     /locus_tag="PKY09_00990"
     CDS             complement(52218..52571)
                     /locus_tag="PKY09_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNB11454.1"
                     /translation="MNIEKFRNYCLSKQEATESFPFDADTLVFKVAGKMFALVSLSEK
                     PMKVNLKMDPEIILEWRERYSEITPGFHMNKQYWNSVIFSDNIPLAELKWLIDHSYAE
                     VVKKLPKKVRMRFEV"
     gene            complement(52654..54117)
                     /locus_tag="PKY09_00995"
     CDS             complement(52654..54117)
                     /locus_tag="PKY09_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11455.1"
                     /translation="MDILRKTIKKLSDEEYQALLLQVSGKKKNKPYYVLEATRNDNHF
                     DDTEMMKQLQVNPSAYYTLKSRLNTRIAEILSKKVENPIRELMEEVVRVPATLYGTNR
                     DFSIRALKEIEKQLKEYDMSNELITVYKTLALLHIHHEDYEHYNKLYNKHVAYALAIS
                     KAEQMFFLFFKKLGAYQLTLDPDTLEEVIMLKRKIANIAEMYDSHRLFVLYNIVRIYY
                     LCSLPSKRQELKSLELEIENILKRINEIFNAFPIDTLYSNISPLVDILHYEYYQKIQN
                     QVRADHYLSNGINDTIIDWSEKPAMHFFIIQYLNSRVEKYLADGEMYRLNEFNQTIEK
                     TFDLNINEPYHYISFKRYIALCKFYAKDYQGAAKTVYDLRNNISLKQFTHTDVDSKLF
                     QGLCYCLMGEDSLAQQMISSIKRQIADFEHEFESTTIFIKILKTALKPSDFRRKIVRI
                     NELLIKFEEANNGDKPILKYIKIDDVLIRKMTNPIKD"
     gene            54503..55087
                     /locus_tag="PKY09_01000"
     CDS             54503..55087
                     /locus_tag="PKY09_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11456.1"
                     /translation="MEDTTMNNTMDFYKMVRENRFAMYSKPRRYYNRDGIKGSAIKFL
                     FFVQAIIFFLMLVTLHSATAQTANTQPALMADAGLNGTIANYTTIYNSGKVFLNWTAK
                     NESADCLYIVERSLDGKVYESVGIKEGLGTAVELFYSWMDQTPPNGYAFYRVKKIARD
                     GSQYYSSTNTVINLDSSYDPKSNYASDKKPEDQK"
     gene            55139..>61018
                     /locus_tag="PKY09_01005"
     CDS             55139..>61018
                     /locus_tag="PKY09_01005"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB11457.1"
                     /translation="MNRYFTTGRNITWKLCFAITLLLGTFSAKAQIPTASFAADSTEG
                     CVPLTVHFSNTSTLATSYFWDFGNGNTSTLPNPQTVYLAPGNYTVKLIAYNSTSGKSD
                     TLIIPNYIDVVSFPTANFSSNVTSGCSPLSVIFNNTSSNFTSCIWDFGDGFSSTQTNP
                     THTYSIPGTYTVKLVAYSSYGCSNVKTQTNYITVYGQPTAQLTANPSSTCDANQVIQF
                     NSSSAGVVSYQWNFGQPSSGGANTSTLQDPTHTYGTQGSYNVSLIVTDANGCSDTAAI
                     NNMITIGTALTPTFTWTNGAGCKPLTTNFTAPSITGATSYAWDFGDPSSGAQNTSTVA
                     NPSHTYANSGTYSVSLYVTTSSGCNGGVILNNIIVVDQPPVAAFTSNPTTGCKPLAVQ
                     FNNTSTGAATYLWEFGDGTTSTLVSPSKVYTTAGTYNVTLHAFSTNGCEKILVKNNYI
                     VVNEIVASYYFSPGSGCAPLSVNFIPQNNAGVVSYEWNFGDPASGALNTSTLSNPTHI
                     YNNVGTYSTNLIVTSAAGCKDTLIKNFVKVRDASVLYTVPDTIKGCQPYPVSFTNPLP
                     GATVFHWDFGVPWLTNDTSNVANPTFTFDSVGIFTVTLNSFMPGNNGSGCSQFFNPVA
                     IVQIFPMLISPITYIQATPCAPYLIHFSDTTMDVVSWDWDFGDGSPHATTQFPSHTYA
                     QPGTYTVVLNIVLQSGCMTSLSTTITLGYPNPITVNKKAGCHDEAFTFGLTGGPWSNI
                     QWNFGDGVGTSTSANPTYTYNQAGTFTVSVTVTSSDGCVNTYTDSITTTNPQPSFVIN
                     GPTSSCTNQFVFFTNTSTGATSYWWDFGDPTSSTGFSSLENPSRNFTTPKAYTISLTA
                     TNGACSRTVTYPALIRINRAAPNFSFTQSSQCFPITVTLTDLTGPTPAIKWGWNFYNG
                     STDSTQNPVYTFYAPPTSDTISLWVRDTNGCSATKRLKGIKYKQAKFTMSATGGCNPT
                     TICFTDASDTTVVSRKWDFGNGATSTQANPCYTYTSDGVYTVTLITTFSTGCKDTLVM
                     TNAVTISTPAADFSVPTQAFCAPALVNFNNLSTNATTYYWDFGDGSNSIATSPSHVYN
                     VPGDYTITLIAINGTCRDTMTKVNYLHVPGTFSYFNLVSLQSCVGNLVQFTDSSLNAQ
                     TWLWNFGDGDSSTLQNPTHLYSTTGSFTVSLITSDNLGCSSHYIFSNPIVVHPVPTAS
                     ATTTDTVGCSPLTSTFTQNSINATTYLWNFGDPASGALNTATTSTATHTYNNAGAYQP
                     YFVAYNNFGCSDTMWLPKTINVNQTPVAQFSSSATNGCTPLTVNFTNTSTKLNTPQYL
                     WNFGNGNTAITSNATVVYADSGSFNVSLIVTNAAGCADTATAQITTVLSPTAIASTTD
                     TVGCSPHTVNFTNVSLNATSYQWNFGNGATSTLANPSYTYNNAGNYTVTLIATNASGC
                     SDTLVFPSQIIVNQTPRARFNMSPATGCSPLTVNFTNTSANTSGATYSWNFGNGNTAS
                     TANASSLYPDSGTFAVTLLVTNGNGCSDDSVRNVVVKLSPTASATTNDTTGCNPYTST
                     FVNNSINATSYNWNFGDGGTSTQANPTHTYPGTGNFTVTLIATNTAGCKDTFVFNNTI
                     QVNQTPLASFTASAVTGCAPLNVNFTNTSTVTSGATYFWDFGNGNTSTVANPSAVFAD
                     SGTYQVVLNVNNGNGCTHTASQLITVNISPTALALTTDTAGCTPHAVSFINNSTNATS
                     YQWNFGNGATSTAVSPSYTYTTGGLMTVTLIASNSAGCRDTLVLPYQINVKQSPRARF
                     NMSPTLGCTPLNVNFTNTTANMQGATFNWNFGNGTSATTANPSTVYSDSGTYAVTLTV
                     TNSNGCSDDSTRTVRANLSPTAIAATTDTAGCAPYTSTFTNSSLNATSYQWNFGDGGT
                     STVATPTHTYNSSGNYTVTLIASNASGCRDTLVFPYAINVNNKPVAAFTPASASGCVP
                     YT"
     assembly_gap    61019..61118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <61119..64079
                     /locus_tag="PKY09_01010"
     CDS             <61119..64079
                     /locus_tag="PKY09_01010"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB11458.1"
                     /translation="SFTNSSTNATSYIWNFGNGLTSTTPNPTHTYTTGGLYTVTLIAI
                     TSNGCRDTFTLPYQINTKQSPLANFSQSATTGCSPLAVTFTNTSTNISGTVWNWNFGN
                     GATSSLQNPTAFYPDSGTYQITLWAMNSNGCMDDTVRTVRVNQTPTAIAATNDTVGCT
                     PYNVNFSNNSLNAASYNWNFGDGITSTTPVPNHTYTNAGTYTVTLIATSSAGCKDTLI
                     LPNNITVNQTPVASFTKSVSTGCSPLTVNFNNTSTQTNSASYQWSFGNGTTSTLTNPT
                     VQYTDSGNYTVTLVTTNASGCSDSTSQQIHVDLSPKAIATTTDTLGCSPHTAVFTNSS
                     INATSYTWNFGDGTTSTTSNPTHTYANTGNYTVTLIVSNAAGCRDTLVLPQTIQVNQT
                     PMAAFTPSATSGCSPLTINFNNQSSDTINANFAWNFGNGTTSNALNPTNVFADSGAFA
                     VTLIITNANGCKDTATHNIQVMQTPTANAFTNDTTGCSPFTANFTSLPTNSDSVVWHF
                     GNGTTASGNNVQATYTQPGIYQPYLIAYNSTGCTDTFYLPKTIRVKLSPVADFAADQN
                     ASCSGTNFTFNNLSSDTLSPVYQWTIGGFSTNNPSFSIPLLTPGFYTVALTVTNSNGC
                     TDSIVKANYIQVFDTLPPPQDPIYSVSVISDTQVEIKWANSAALDLGAYKLWRLDPSS
                     GNYVNVYTDNNPSNSTMNPESKYTENGLNTLNNTYTYKLQTLDRCSFALPLNVLKAHT
                     TINVSANKSSNGINVTWTPYQGCPVSQYEISRTEVSNGSSAVIATVPASTLYYFDSTM
                     ICPDDYSYRIKAYDLCGTTYTSYSDTAAAKPDYNLQEQQAIIVRSTVVNNNFVLTEWS
                     EPRLHPERVMQYNIYRSTDTTGNFYSLLATVSANVTSYEDFAVNVNEQNYYYKIEVIS
                     DCNMAGQLSSNSSSILLQSKWEYANSRLWWTKYDKWDTGVERYEIEKYNWGTGQWEKV
                     KTVPGNATETEVDE"
     gene            complement(64236..66167)
                     /gene="dxs"
                     /locus_tag="PKY09_01015"
     CDS             complement(64236..66167)
                     /gene="dxs"
                     /locus_tag="PKY09_01015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNB11459.1"
                     /translation="MEIKAGELLSSINTPDDLRKIKPEQLEQVCKELRQFIIDIVSVN
                     GGHFGASLGVVELTVALHYVFNTPNDQLVWDVGHQAYGHKILTGRRDVFHSNRIYKGI
                     SGFPKRSESVYDTFGVGHSSTSISAALGMSVASRLKDDKSRQHIAVIGDGAMTAGMAF
                     EALNHGGVENSNLLVVLNDNCMSIDPNVGALKEYLTDITTSPTYNKVKDEIWKALGVI
                     SKFGPNAQAIAQKIENAMKSAVLKQSNLFESLRFRYFGPIDGHDVNHLTKVLEDLKNI
                     PGPKILHCLTVKGKGFKLAEQDQTKWHAPGLFDKITGEIFKPQNNTPQPPKYQDVFGH
                     TMVELAEKNSKIVGVTPAMPSGCSLNIMMKAMPDRAFDVGIAEQHAVTFSAGMATQGL
                     IPFCNIYSSFMQRAYDQVVHDVALQKLNVVFCLDRGGLAGADGPTHHGAFDIAYMRCI
                     PNMIVSAPMNEEELRNLMYTAQHDNLGPFCIRYPRGNGVLTEWRTPFKAIPVGTGRKL
                     RGGEQVAILTFGHPGNFAVAACDELAKENIYPAHYDLRFVKPLDQMLLHEVFANFKKI
                     ITVEDGCLQGGMGSAVAEFMIDNGYAAEIVRLGIPDQFIEHGEQSELYAECHFDMQSI
                     MKTVRQLIAASHHGIKSLK"
     gene            complement(66301..67047)
                     /locus_tag="PKY09_01020"
     CDS             complement(66301..67047)
                     /locus_tag="PKY09_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB11460.1"
                     /translation="MIEISHISKKFFDGEKPVIALYPTSLRIKEKEFVIIVGANGSGK
                     STLLNIIAGTILCDEGKIAVDSNDITALKEHERSRFIARVFQNPQSGTASDLTIIENF
                     RLAALRSHSKILRIGIDKKFVDSIRERVSWLGLGLEDQLNRKMGALSGGQRQALTVVM
                     STMDDFKLLLMDEPTAALDPKSAELIMKLTSRILNQMPVAILMVTHQTKQVLDYGNRI
                     IQMASGQIIRDLDSTSKEKLTLNEIIHWFD"
     gene            complement(67044..67895)
                     /locus_tag="PKY09_01025"
     CDS             complement(67044..67895)
                     /locus_tag="PKY09_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11461.1"
                     /translation="MEVIIPAIIQGFCYAAMAFGIYLTLRIFNIPDITTDGSYTLGGV
                     VAAVLISNHWHPSYAMPIVLVAGALAGVATGLIHTQLKLHPLLAGILVMTSLYSINLF
                     IMGRPNIPLINEANIFNDNTSFNMLLAAMFSIVLLLLLFWLMKTDFGIAMRATGSNEL
                     MVKAMGINTNFMKVAGLAIANALTALSGYMMVQYQGFADINMGIGIVIAGLAAVILGE
                     SFTAFFKSSSILSALISVLLGSVLFRLLLAFALLAGVPPQMLKLLTAILVLLAAGVFK
                     FIFRPAQ"
     gene            complement(67895..68854)
                     /locus_tag="PKY09_01030"
     CDS             complement(67895..68854)
                     /locus_tag="PKY09_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB11462.1"
                     /translation="MKNLHLIIVAICCFVSSCDNTSKNIPSIGFLDIAEDATLAKART
                     GFLDALAKNGFSEKDKTLKVYFSNAQGEIAVLNQACDYLISKNVKLIAANSTLSTIAA
                     VQHTKNIAVCMMVAPRPDLAGLTDAQGNYPKNLFGVFETLAYIDSSVALIKRFFPDAM
                     TVGTIYNSSEPQSRDALEELQKAATAAGMKIIAMPVTNSSETQLVTQSLIAKKPDVFF
                     ALPDNVIFASFETVVKACNEAQLPVFTSEAGLVGRGAIASYGADFYQWGFQAGVEAAN
                     YLKSNPATMLKPEIVKVRQYVFNNKAAARFNLVAPNGFMVIVP"
     gene            complement(68958..69152)
                     /locus_tag="PKY09_01035"
     CDS             complement(68958..69152)
                     /locus_tag="PKY09_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNB11463.1"
                     /translation="MSSVTCLLLWSLGGSEILLIVLVLLLFFGGKKLPELAKGLGKGI
                     KEFKDASSGVEKDEEKKENK"
     gene            complement(69215..>69820)
                     /locus_tag="PKY09_01040"
     CDS             complement(69215..>69820)
                     /locus_tag="PKY09_01040"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB11464.1"
                     /translation="LQASEKSLRKKLEAKIRDKVKLDKAIAALVRKEIEAAKKKAVKA
                     GKKNVTNENVFTLTPEAQQLSNSFANNKGSLPWPVEKGRISETFGEHQHPLLKNVTTK
                     NDGIDIRTSKGSSVRSIFNGEVTGTVNLPNGSAVIIRHGEFLSVYSNLDEVFVTKGEK
                     VTTKQKIGRVRTDSESGNADMNLQIWKGFSKLNPALWIAKN"
BASE COUNT        22446 a        13673 c        12203 g        21398 t
ORIGIN      
        1 gcccggatca acattgatgt aggggtccag tttttggatg gtaacccggt agccgcgtgc
       61 ctgcaacaat ttggcaagag aagcagaaat aattcctttg cctaatgatg aagaaacacc
      121 accggttaca aaaacatact ttacagcgct cataagtaag gttgtggtga tacgggatgc
      181 aaaagtaatg ttttgaatcg gctttattga aaagaacttc aataaatcaa ctttatttaa
      241 ttacttttta catttcatgt ttttacaaat tatgcaattt aaacctgtta caagaattct
      301 cagttaaatt ttacttatca ttaatacttc tatctttaag agcgaatttt aactacaaca
      361 aaatcaggaa aataagaact gaatgttttt atatttgtaa aaattaataa acactaaagg
      421 agatgaaaaa actatatgct tttctaattg caatgctgat atcatcattt acgaattcca
      481 tatcagctca gagtcaaaat gctcttgatt ttgacggcat agacgatcag gttgtttctg
      541 taaatgcctc gtcactcatt gccaattcac cgcaaatttc acttagcttt tgggtgtatc
      601 cacgtaatcc tgttcccgga tttcctgact ttgacggact ggcagggttc agagataata
      661 cagtggccga tttctatgtt ttacagtatt catccactgc cgttgaagct cgttttcgaa
      721 atagcgcagg tataaatttt gacattcttt cgcaaggact gcaactcaac acatggcagc
      781 aatatgcgtt aacttatgat ggctcttttc taagactttt tagaaatggc ttaattacag
      841 attccgtttc cgcatccggt gttatttcca gcactgctac atcatttttc atggggaatc
      901 ttgtttttag tggtactaat tttcttcttg atggtcagat ggatgaagtg gcactttgga
      961 acagggcact tacacctcag gaagttttat gtatttataa aaataaagtg gatgcaactt
     1021 cccccggatt acaactatat tatgacatga acattggcat agctggtgga aacaactcta
     1081 ctatacttac cgttcctgat atttcaaatc atatcaatgg tgttttgcaa aattttagca
     1141 tgacgggcac atcatcgaat ttagtgaatg gcattgacaa tgcaactatc attacagata
     1201 caatttgttc cggtcaaact tattctttta acggacaaac actaacacag gcgggtgttt
     1261 acacagcaac cctggtttca tctgccaact gcgactccat tgtgagactt acactttttt
     1321 cgaatgatac tacagtcaat cagaatcagg cattattaac agctgtgcag aacggagcaa
     1381 cttatcaatg ggtagattgc agcaacaact ttagtcctat accgggtgca accaatcaat
     1441 cctatacagc cacagctaat ggcagctatg cagttatcat cacagcaaat ggatgtactg
     1501 acacttcagc gtgtcacaat gttactacag taggtattaa tgattattcg gcttcaaatt
     1561 ggataacgtt ccctaatcca actgttaatg ttttcaatat tgttacgaat gaaataataa
     1621 acaattccga aataattatc accgacatct cagggaaaca aatcagcaga atgactaaag
     1681 acataaaata tcattgtgct gttgatgttt cagctttgcc cggtggctct tacattatta
     1741 cattaaaatc cgaaaagaaa tctaaaaatt ggcgattgat taaaatttaa agataccgtc
     1801 aaaacaagta attgtaattc cggcttcctg aagttataag gaataaagat tactctgtat
     1861 catgatcttc tgctttcagt ttgaagccaa gttgtttact gcttgcaaca gcagagccaa
     1921 acgatccgga agaaatttca cgataactca tagcaggaac ctgatcgaaa ggttgtgcaa
     1981 acaaatcata ttgtaagcaa aataaaatta cctgttcgag tacatcattc aatcgttgct
     2041 cagtcaaaat atcattactg atatcgttaa acaacatccc caactgcttg cgtccttcta
     2101 ccataaaatg attttcgtag ttgacaaaaa ttctggcaat gagcatacca taatcatcac
     2161 tcctgcgctc ggcaagtgaa tcagacagaa aattataaac atgtatcact gcacagcgac
     2221 tacgatattc atcatcgcgc agataggaat aacgataagc tggattatca gggcttatgc
     2281 gaaacaaatt tggatgcaaa tgaaaccata acgtgtcatc acccaattgc aattcgcact
     2341 gattgctgcc agtatcataa aattttatac gctgtcgttt gtttggtgcc tgataatacg
     2401 ttgcagtagc atcaacaaat tttttaagat agttttttat ctcattaaaa tgccctgttg
     2461 taaattcaac tattttacag ttcagaggaa aatgtgtttt aatgagttgt gctatcttat
     2521 cgtttgccgg ttccataatt taatatttta ataagctcgt gcaaataaca ctctctgtgt
     2581 gctgggtttg cccgtaagaa tgcatttgcc tttttcttgt ttgttgttaa gtggaataca
     2641 tcggatagta gcttttgttt gttctttaat tttctcttct gtctccactg taccatccca
     2701 atgtgcaaat acaaatccac ccttagtgtc gagtatttta ataaactcgt ccatgctgtc
     2761 agctacatgc gttgatgtat cgcgaaagtc aacggctttc ttaaaaatat tctgttgaat
     2821 ttgttcaaga agattttcaa tctttaattc aatatctgtt atctgtacag tcattttctc
     2881 cttggtgtcg cgtctggcaa gttccactgt gccactttct acatcacgag gtccaattgc
     2941 caatcgaaca ggaacacctt tcatttcata ttcggcaaac ttccatcccg gcttggcttg
     3001 ttcatcatca tcataaacta cgttaatgcc acgtgcggta agtgcctttt tgattttaag
     3061 tgcaatttca gaaactagtt tcagctgctc atcattctta taaattggta caatgacaac
     3121 ctgaatagga gccagcttag gtggtaacac caatccgtca tcatcagaat gtgccatcac
     3181 taatgcaccc atcagtcgtg ttgaaacacc ccatgatgtt gcccacacaa aatcctgtac
     3241 accttgtttg gtagtaaact ttacgtcaaa tgcttttgca aaattctgcc cgagaaaatg
     3301 tgatgttcct gcttgcaggg ctttaccatc ctgcatgagt gcttctatgc aataggtatc
     3361 caatgcaccg gcaaatcgtt cgttagcaga tttcacacct ttaacaacag gaatagctaa
     3421 ccattgttca gcaaattcag cataaacatc aagcattttt cttgtttctt caacagcttc
     3481 ttcagctgtt gcatgtgcag tatgtccttc ctgccataaa aattctgcag tacgcaaaaa
     3541 caaacgtgta cgcatttccc atcgcaccac attagcccat tgattgagaa gaatgggtaa
     3601 atcacgataa gactgaatcc agtttctgta agtattccag ataattgttt ctgatgtagg
     3661 acggacaatc aactcttctt caagctttgc ttccggatca acaactacac tttttccatc
     3721 atcggattgt ttcaaacggt aatgtgtcac aacggcacat tctttggcaa aaccttcaac
     3781 atggtttgct tccttgctga agaatgattt tgggatgaag agaggaaaat atgcattgct
     3841 gtgtcctgtt gctttaaaca tgctgtctaa ctgttgttgc atgcgctccc aaatggcata
     3901 accatagggt tttatgacca tgcaacccct gacagatgaa ttttctgcaa ggcctgcacg
     3961 aatgacaagc tggttgtacc actctgaata attttgctcg cgtgttggaa agtctttagc
     4021 cattttttat ctttgaagtg aaattgcaat gaatgatttt tgcaaaactg ttgaatttta
     4081 tgagatggca gcaaaattgt aaaacaaaca taacatatcc aattacgtta aactattgtg
     4141 cataaaaaca tgtcaaataa taaaaatcaa aatacgatga aatcaatagc actatctgca
     4201 gcagcaatat ttacacttgc ctcctgcacc tcatcacaac aggcttcaaa tgcctattcc
     4261 gatgacgttt attattcatc caaagcaaac aaacctgttg aaaccgtgca gacacagcaa
     4321 caagtgcagc agcctcagca ggatgccaat aatcaaacag aatccaatca gggcagcagc
     4381 acttctgctg aaaaccgttt tgattataac gatgcaggca caacaacaca ggatggcaac
     4441 acctatgtga ccaacaatta ttacagcgat gatgattatt atgactatgc ctactcatca
     4501 cgcatacgca ggttttatcg tcccatgtgg aattatggtt attatgattc atattatact
     4561 aattcatact ggtatgatta caacccttat gactatggtg tgagcattta ttgtggttac
     4621 aactggtggg ctccttcatt ctggtgggct ccttcattca cttatttcag ttggggatat
     4681 acaccatata actactggtg gagcagtcct tattcatact atggatatgg tccttatcat
     4741 catcatggct ggaacaatta cagctatggc tactggaatg gctacaacaa cggatactgg
     4801 aatggttatt acgatggtta ctataatggt tatggttaca atccatacta tttcaacagt
     4861 tatgacagtt atagttacaa caacacatat tacggaccaa gacgctcgac ttcaggctct
     4921 ggcagcagca atactgttcc gccaagaagt atagccgatt catataacag agctttggtg
     4981 aaaaacggta gaaactcaat gcctgtaagt caaagttttg ccaatgcaat gtcattgcca
     5041 acacgagata caaaatctac agaggcagaa gtaattcacc caagaaataa tgtgaacacc
     5101 aatagtccgg taaaagattc tcgcaatgtt gaaaccgcac ctgtaaggaa taatgagact
     5161 gaagtaattc acccaagaaa taatgcgaac actaatagtc cggtgaaaga ttcacgcaat
     5221 gttgaaacag cacctgttaa aaacagcaga gccgatcagt tctctacacc acgaaataat
     5281 ggcgatgtaa aagacaactc aaatactcct gttgctcctc ctgtgcgcaa caacagtaaa
     5341 aatgaagtta atccggagta caacagtcca cgcaatacag aaactgttcc tccacgtaat
     5401 tacgaaagta atccacgtta caacaacaat actcctgcag agtctccaag aagaaatata
     5461 gagagtaatc cacgctacaa caacaacact cctgcagaat ctccaagaag aaatatagag
     5521 agcaatccac gctacaacaa cactcctgca gagtcaccaa gaagaaatat agagagcaat
     5581 ccacgctaca acaacaatac tcctgcagaa tctccaagga gaaatataga gagcaatcca
     5641 cgccataaca gaaacagcga aacagctcct cgtcaggaac aagcgccacg tcaggaaaga
     5701 gtaacaccga ctccacgtca ggaacgtagc ttctctgcac catcacaacc acgtcaggaa
     5761 tcacgtccac gacaagagtc aactccttcc agaaacaatt cacgcaacgg aagataatta
     5821 tcagtaaaca acaattaacg acattatgga ttatagattt aaaagcatgc ttgcggcatg
     5881 ctttttttta accgcaacag catttgcaca gaacgaaaat gatgcgctgc gctacagcat
     5941 gcaacaaaac ttcggcacta ctgcccgctc acttagtatg ggtggagcct ttggagcttt
     6001 aggtgccgat ttttcaagcc tttcaataaa tccggctggc attgccgttt atcggagaag
     6061 cgaatttaca ttcagtcctg tcttacaatt cagaaataat gatgcttctt attcaggcac
     6121 attaaccacc gacaacagag ccaatcttgg tattggcaac cttggttttg tacttgcatt
     6181 tccgcgcgag aaaaaaactt cgggatggat gggaacatca ttaggatttg gttacaaccg
     6241 tatcaactca ttcagcgaca ggtttgcttt tgaaggaatc aataaaaata attctatcaa
     6301 cgattatttt gcagaaatca gcaatggaat aagcaaagct aatctctccg gtgatgaaat
     6361 gtatcaattt gatgccggac ttgcatacaa cagctatctt acaaatccgg ttgccgatag
     6421 tagcctcatt gatcaatatc agggtgctct ctttaatgga gatttaaaac aaaaaggtct
     6481 catcacacgc acgggttcac aaggtgaaat tgatatttct tttgctgcca actacgacaa
     6541 taaagtttat cttggtgcaa ccgttggtat tccttatttc agatattcat caacggaagt
     6601 ctatgaagaa tatgcacttc aaaacaccat taaagacagc ctgagcggta aaactttcaa
     6661 aaattcaaaa tacacacaaa agctttcaac acaaggtaat ggcgtgaacc tgaaattagg
     6721 tgtcattttc aaacctactg atatggtgcg tttaggatta gccatacaca caccaacata
     6781 ttatgaaatg aatgatcagt attcttcaaa aatttatgct aatttttctg aaggatattt
     6841 agaaaacaaa tcacctgacg gacgattttc ttataatctt accacacctt ttaaaaccat
     6901 tgcaagtgta ggattggtat ttcttgatca ggggctgatt agtgcagact acgaattttt
     6961 agattattct gtggcacgtt tcaactcaaa tgattataat ttttctgatg aaaacagaga
     7021 tatcaaaaat cgttttgagc aaggtcatac tttaaagatt ggtgcagaat accgatataa
     7081 agtttttagt ttcagagcag gaactgttat gcagtcaagt ccttatgaag gaggaataac
     7141 ttacaaagaa ggtaaccgta gtgcagtgag ctacaccggt ggcataggaa ttaaaggagg
     7201 aaactttttt cttgatttag gttattcttt taccaaaaca agtggcaatt atgcgcctta
     7261 cacattagca gacggcagtg cgccatcagc atctgttgct accaaaatca atcgctttat
     7321 gatgactttt ggtgcgaagt tttaaaagcg attacttttt attcagtcct tcaatcacac
     7381 tttgtgtaat atcaagcgaa tcgttggcta ataaaattcc gttgccacgc tgatagccga
     7441 aaacaaactt aaaattagtt tgcttattca tgtcctttaa atagccaact aagtgtttgt
     7501 gcaaactatc ttgcatgaca gcttcttctt cagtcaactg atcgagtaaa tggtttttat
     7561 attcaaccaa ctgttgttgt ttgtgcccta atctttcttc ttctgctcca cgttgctgat
     7621 ctgtcatgct ggcaccacgt ttctggtaat taacaatttc accttccagt tccttgcctt
     7681 tgtttttcag aattacatct atactgtctt gtttcttttt tagcgattca cgaagactaa
     7741 tgtaataatc gtagtgctcg agtaaggtat ctacatttac aaaaacaata tcaccatcat
     7801 tcatggcagt ggtaacacct gcaggagcac aattgctcgt acagctgttt actttgtaaa
     7861 acaaataagc tactgccaga gtaagcagta cacagtaaat taaaagaccg tttttcattt
     7921 ttttaagata aagattcaaa tagcgtgcaa agctatcaaa ttaggcatat caatggtaaa
     7981 acaagtaatt aaaccaatac caaaatcaaa tttttactgt caaaatctgc aatcaaaata
     8041 ctttttctat tttcgtgaag tatgcaaatc attgaaccgg tcgaaaggca cctgctggaa
     8101 caggagttaa ataatggccg ctttatccgc aatgccaaca atgggaataa taagttgtat
     8161 gtggttacag ctcatgattc gcctaacgtg ctgaaagaaa ttggcagggt gcgcgagctt
     8221 actttcaggg ctgccggtgg tggcaccgga aaagcatttg atttagatga gtttgacctg
     8281 attgaaaatt gctacaaaca acttatagtt tggaatcctg ccgacaagga aattgttggc
     8341 ggctatcgtt tcatacgctg tgccgatgca gccactgacc cacacagtca atttaaatta
     8401 gccacaacag agctatttca tttcaccgaa aaatttatca atgattattt tccgcatacc
     8461 attgaattag gccgttcgtt tgtgcaacca caataccaac cttcggctga taacagaaaa
     8521 ggtctttttt cgctcgataa tttatgggac ggactgggtg ccatagccat tgataatccc
     8581 gatatcaaat atttttttgg gaaggtgact atgtatcttc gtttcaaccg tattgccaga
     8641 gatatgattc ttgcctttat gcaacattat tttcctgata ctgataaaat ggtttatccg
     8701 catcatccat tgccgtcaga aaatgatgtt agtggttttg tagcaagcct aaaaaatctt
     8761 ccttacaaag aaggacatca tattctgaat aaaaaagtgc gcgagcttgg agaaaatatt
     8821 cctccgctgt ttaacagtta catgaatctt tctgctacaa tgaaaacttt tggcactgcc
     8881 ttaaatcatg aatttggcga tgtggaagaa accggtattt taatttctat tgatgatatt
     8941 tacgaaagta aaaaagccag acatgtaaac acttatcatc cgacacttta aaactgcacc
     9001 ttaccgctgc cattaaaaag tgtagtagtt gtaaactgca accggaatgg tgcatcgcag
     9061 aaatcaaatg tgtttaatgc ttttaatttc accgtaccgc ttgctgcctg ataggtgcct
     9121 gatgcattgc tgaattccac acaaacttca ccgggtgcaa gtgctccact gccacttgca
     9181 ggacaatgtg caggattaat tgtatagttc cctgtcggag gattgttacc atagaacgta
     9241 gcacgcaatt gcgaagttcc atttagccca gttcctgtaa tacttctggt gttgggataa
     9301 aggttagcac tgccatagga gtacagaaaa taattactgt ctaatgagtt ggcaaatgtg
     9361 ttggatgatg ggttgcactg attaaaactt acaaccacac tgtcaggctt gctatagcaa
     9421 tgatccttgt gtgcataaac tgtataaaca ccttcgttgc cgggaatggc attgatgatt
     9481 attggattgg gttgggttga agtaaatccg gcaggacctg tccagatata ttctacagaa
     9541 tcggtaatag aatgtgcaaa cagatgaatc gtatcgttgg tttgaatggt ggtaatataa
     9601 ttagcaagaa catcttccgg acgatcgcaa attacagtaa cataaaaaga gtcaacatta
     9661 cctttacaga gtccgttgat gtctgcactt gcatagtaat acccctcact taatgatgaa
     9721 gattcggaga tgacaggatt aaaggtgttg gcaacaaaac cattaggccc tgtccaggag
     9781 taggtagcac ctgtaaacaa agttgccact aagccaatgc tatcaccggc aacaactgta
     9841 gtatcgccac tgatgtgcat ctccattgcc ggacaaggcg gattatcaga acttttatcg
     9901 ttatgacatg aggtcacaat gattagagcg aaaaccaaaa aataaaaagt atttctcatt
     9961 gtaaaaacgg atacgaaact acatgattcc ggttgaagat taaaatttac ttttgccctt
    10021 gattaaaatt atggaaataa gttcagcaga atttgttcaa agcaatacag atgtaaaaaa
    10081 gtgcccggct gacagtttgc ctgaatatgc atttatcggg cgaagcaacg taggcaaatc
    10141 atcactcatc aacatgcttt gcaataaaaa aggattggcc aaaacatcct ccacacccgg
    10201 aaaaacacaa ttgatcaatc attttatcat caacaaacaa tggtatctgg ttgacttgcc
    10261 cggatatgga tttgccaaaa ctgctcgtac cgagcgcgaa aaatgggacg gtatgattcg
    10321 caattacatg atttaccgca aaaatctgat gtctgttttt gtgttgatag atatcagaat
    10381 tgcgccacag caaaaagacc ttgattttat caactggctg ggcgaaaaaa atattccttt
    10441 cagcatggtt tttaccaaag cagataaaca aacacgtgct aaaaacgatt tttctgtaaa
    10501 gcagttttgc gagcagttat ataaaacctg ggcaacattg ccaccatatt ttgtaacttc
    10561 tgccgagaca aaaagtggcc gtcaggaggt attggattat attgatagta ttaatcagtc
    10621 ctttaaactg aatggcacac aataagaacg gaaaagctac tttcctgcat cttgcctatt
    10681 ggtattttca gttgcattag caacaggcaa cagtttaaaa taagttgctt taaaacggtt
    10741 gtttaccact tcaccctgaa cttcggcttt gcgaacagct acacaaaaac cattgtcatc
    10801 gtgcggatgt ccgaattcaa aaatcgaaac accatcaaca tacatcgctt taccattaat
    10861 gcgtacagcc aaatcgcagt cattaccggg taaatcaaac ttacactcac cacaagtggc
    10921 ttcaacgctc atcaacgctt ttgatgaatc gggttttgaa gccaatttag aggccacctg
    10981 tgcctgaaca ctgagagaaa acatacccaa caagaatgaa agaaataagt acttcatgat
    11041 atcctattgt ttaaaatgaa acactacaaa tataaccaat tatatggtgg gaatttatat
    11101 acctatttct ataatctccc atggcttacg aataggtttc tgtgatgatg atagaaataa
    11161 aaacacttta cgttcctctt acataagttt taacttcaga caaagaattg caggtggtta
    11221 ttagggatta ttataacaac tgtattattt tatgttgcga aaggaagttt acagcagcaa
    11281 actgaagcgc agaaattaat aattaaagtt ttttcttttt aatcgacata ttcctttttc
    11341 atctacttta gcaaagagaa acaaaaatga aaaggttttt aattaataaa acaaaaaggc
    11401 acattttgca aaatggcgca ttgtcctttt tatttttagt tagcaaattt ttgtgtggat
    11461 gcacggtttg cggatagagt aaaattctat ccaagttttt tttcttcttc taagtcaaac
    11521 tttttaatca acatccaaat gaaaaatcaa tccaaaataa ttattgttat tgccgttctg
    11581 tttatttcaa ccacaaatat taaagcacag aacaatgcca tcattgatat aaatgctcaa
    11641 ttaagaggct tattttctcc tcttgccaaa cctaatccgt ctttagaatt tctttacgat
    11701 atgtcagcta aggtttctga tagtgttttt tatacaacgt tttgcaaaga cactttagaa
    11761 actccaatgt ggcaaagtat ttatgacgag atgtatcata ctgcatacga tacatcttgg
    11821 ttgcccattc ctgacgactt ctttttatca ggaaataatt tttattctga tacaattcca
    11881 atgggtatct tgaattttgg tttctacaaa tttcttgatg atgcaatgac cacaaacatt
    11941 tattttaact ttgatactgt aaatgatata ttaacagata agtatccgcg tcccggcttt
    12001 ccttacattg atagcaattt atttgtagct gccccaatga ttgcgagtgc tcaattttca
    12061 aatccggtat atagagttga ccctcagttt attttgtttg atgcgctgaa tgctggaatt
    12121 tacctgcatg gagaaggtat tctgaaaatt gattttggtg atggaaatgg ttggatacag
    12181 tttgacccaa ctattattac caactggcat gtccagtatg cttcagcagg tgaaaagtta
    12241 attcaaactg caatattcga ccatggtaat gacatgcttt tttactcgct ttccagaatt
    12301 gtgattgttt caggcacagc aagtattccg cctgaccaat atattgaatc cgaagggtta
    12361 attgtaggat attatggtgg ttgcaattca caaggtatct ctgcaaaaac tgttatctat
    12421 ttatcaggta tagatatatt agattttatc ccatcaaaaa acagaaccat tgcaacaatt
    12481 tattccgaaa tgattaagca ggacaatatt gttcagctca agaatcaagg ttatggcttc
    12541 atagttgttg actatgcaaa tagcaggatt gatgtaaggt ttaatgcact ctattttgtt
    12601 aatttattag aaaatctaaa atgtctttta caagatataa accagcagca atttgttgta
    12661 atgggtgaaa gtatgggagg attagttgcg cgttttgctc tgacatatat ggaatcagaa
    12721 tattatatgc aacaaaacac atctccgttt tttgttgacc aatttgattt aaacaacatt
    12781 ccctatttgg cgctgcatcc gaatatttat aatatgcctt cacagtggtg tcgcacagaa
    12841 aagatgcaca acaccagact tttcatcacg cttgatacac ctcatcaggg agcaaatatt
    12901 ccaatgtcta tgcagtacgc ttacaaaacc ggaatagatg tcctaagcag atttataagt
    12961 tttgatttaa gacttttaac gaacgctttt aatctttttc ttgacgggca agcagcacaa
    13021 caaatgctga tatatcacat tgatacaaaa tcaggaagcc cattctatta taattatgat
    13081 tcaaaaagcg acaagtattt tttctttgac caattaaatg ctttagaaaa ttatccacag
    13141 tatgctaaag tgattgcaat gtctcagggc tcaatgtcag gcgaaatgca aagaaacaac
    13201 tacacaggta gcgaccgcaa ccccggtgac aggctgttgg attttagtgc agatttgtat
    13261 gcaagagttt tatggctaaa agtaccaatt ttcggaggtg acttagaatg caaaaccaat
    13321 cctaatggca acggacatat ctttcttgct caggcaggtt ggtatggtat aagaattaaa
    13381 ttgaaatggt ttggaattaa agttactatt ggatataact ctttacttta tgtaaatgaa
    13441 tatgcaaact tacttcctta ttgcgttaat cctggtggaa tttttggcga ccctgatttt
    13501 ataagtacaa caccatctca aggtggattt aatctctcaa acaattattg gcttcttaac
    13561 ttttttcatt gcagccatac tgtaaacggc aatggttgtg ttgactttga ttctcactta
    13621 ggactaaacg gttttctttc aactaatttt gattattcgc tgtgctctga tggtttgcaa
    13681 tttggatttg tacctgttca gtcagcattg gattatggag ttctcggagg catcccttta
    13741 aattttgata ttgtttccga aaatattaac acaaaacttt caaatactcc aaccgacatg
    13801 atgattggtg catacaataa cttcacagcc acatacccca atcttagcca tgttggattc
    13861 agaaacaatt atgtctataa tctcaccgac caacctaaca acgggcctca tttttgtact
    13921 tactattctt gtattggtaa taacgaaaat gttgagcgta gcttgttaaa ttccgaaatt
    13981 ggggatgaaa atttatttct tgaaaacgta accttgcatt gggaaacaga atatcaacct
    14041 gagtatgata tgtttgttaa tgttaggaat ccgcggtatg aatatccaag tcagccatat
    14101 tcaaatttaa attatatgtt tctgggagtt tattccaaag aagacccgta tataattgac
    14161 aattcaggct acgcaacctt ctattttgat ggagccaata ccccaactgg tacagggttt
    14221 gtttacgacc caccatacac aggaaattac aatcatatag accaaccgct ttccgtttgc
    14281 tgtgaaaact ttatggaacg aaaagcaaac acaactaaga agaaaccaaa ccttgtaagc
    14341 agtttggaaa tttttccaaa tccgaatgat ggacaacaag ttgttttgaa ttttcgcttt
    14401 aaagaatatg ggcaggtaaa cttttcttta tacaattcga caggtatatt aattggaagt
    14461 gagatactca aaactaaaga tgcaagcact ccaacgactt ctgttttaaa tttaagcaat
    14521 agaatttttg catcaggctt atattacatt caactcataa gtgataaagg ggaaactctt
    14581 tcacaaaaat tattaattaa taaataagtt aaatcaaagc agttcgcagc tatgcgaact
    14641 acttttaatt tttattgaaa atgaaaaaat atataataaa tataaaattt cttgcattgt
    14701 caagtttagt tctaatcttt caatcctgta agaaagatac agattctccc ggtgatggac
    14761 caacttatac aattactgga cgattgtttg aggattgcaa tatgcagcct gtcgcaaatc
    14821 agaagattga tttatttcaa gagtattcaa cagggttaga tggtaatttg aatggaggga
    14881 ttctcgctca aactacaact gatgcaaatg ggaactttaa gttcgacttt aaagatatgg
    14941 atggattcac tgagagcatt agaattccag cagggcaagg ttatacaaag ttatttcaaa
    15001 atattccgca aaatcaaagt ctatttgatt taagagtata taaatcccct accacaaata
    15061 ttaaagttaa cttaattgtt aataatagct atacaaattt agatactctt cggcttacag
    15121 atttcaacaa tcttagtaca ccttataaaa tttcaggacc atttacaagc agtttattat
    15181 atgaatcagt agattttccg atactaaatt ttggttatcc gcaaaacatc ttgaactttg
    15241 gctataaatt aaacaacaat tcagaagtta tgagcacgcc aacaataacg acctgtggta
    15301 ttactaatat tgaggttgtg attaattaaa agaataaaca ttagtataat attcaatcca
    15361 agcagttcgc atagctgcga actgctttta atttttattg aaaatgaaaa aatatataat
    15421 aaactaagtt ctaacatcaa gtttcacaca aaaatacttc tttaggaggt ttgaagttta
    15481 attttttacg tggtctgttg ttcaagtcat tttcaatttg tttaatttga taatggttta
    15541 tagtattaat cagtccttta aactgaatgg cacacaataa gaacggaaaa gctactttcc
    15601 tgcatcttgc ctattggtat tttcagttgc attagcaaca ggcaacagtt taaaataagt
    15661 tgctttaaaa cggttgttta ccacttcacc ctgaacttcg gctttgcgaa cagctacaca
    15721 aaaaccattg tcatcgtgcg gatgtccgaa ttcaaaaatc gaaacaccat caacatacat
    15781 cgctttacca ttaatgcgta cagccaaatc gcagtcatta ccgggtaaat caaacttaca
    15841 ctcaccacaa gtggcttcaa cgctcatcaa cgcttttgat gaatcgggtt ttgaagccaa
    15901 tttagaggcc acctgtgcct gaacactgag agaaaacata cccaacaaga atgaaagaaa
    15961 taagtacttc atgatatcct attgtttaaa atgaaacact acaaatataa ccaattatat
    16021 ggtgggaatt tacttttcta tatcaggagt gccccctgtc agcaggaaag aaatcggtta
    16081 gattagtccc tttgatggcc cacaattagc acatttcagg cttgtacaac ctatgaaagt
    16141 tgcagcaagg ctgctaataa tttgtccact tttttcttgg aattccacac gagggcgggt
    16201 gggaatagca gcaaaggcag catataacct tttctacaga gtgaccaatg gatttacttt
    16261 ttacctttgc aatctttgta aaaacagtga taatgaatca taaaccatat cctgaattca
    16321 agcagttaga ccttgccggc attgcaaaag aaattctcac aaaatgggat gccgaaaaaa
    16381 catttgagca gagcatcact ttgcgtgagg gctccggggc ttttaccttt tacgaaggtc
    16441 cacccagcgc caatggtctg ccaggcattc accatgtgat gggacgtgcc attaaagata
    16501 ttttctgccg ctttaaaaca ctgcaaggct atcaggttaa acgcaaaggc ggatgggata
    16561 cccatggctt acctattgaa ttaagtgtgg aaaaaacctt aggcatcacc aaagaagaca
    16621 ttggcaaaaa aatttctgta gaagaatata ataaagcctg ccgcaaagag gtgatgaagt
    16681 ataaagaagt gtgggatgac ctcacccgta aaatgggtta ttgggttgac cttgataagc
    16741 cctatatcac ttttgaaaat ggatatattg aaacttgctg gtatttgtta aaagagctgt
    16801 ataaaaaaaa tcttttgtac aaaggctaca ccatacagcc ttactcaccg gctgccggaa
    16861 caggactcag ctcgcatgag ttgaaccagc ccggaaccta taaaatggta aaagacacta
    16921 ctatagttgc gcagtttaaa attaaaaaag aatcattgcc agaaaactta gcatcacagc
    16981 ttagtgatat taaaagtgat ttttactttc ttgcatggac caccacaccc tggacattac
    17041 cctccaacag tgctttagca gttggcgaaa aaataaatta tgtgtgggtg caaacgttta
    17101 atcaatacac caacaaacct attgtagtag tgcttgccaa agatttgttt gcaaaatatt
    17161 ttagtgagaa aaatacagca cttagtcttg atgattttaa acccggtgac aaacaaattc
    17221 cttttaaagt agttgcagaa tgtaaaggtt ctgcgctgac aggaattgtc tatgaacaac
    17281 ttttgcccta tgtagaacct gatggaaagg cttttgttgc cgttgcagga gattttgtca
    17341 gtacagaaga tggaacaggt attgttcacc ttgctcccac ctttggtgca gacgattttc
    17401 gtaccgccaa acaacaaggc atagaagcta ttttggtgaa agatgaaaca ggaaatgcca
    17461 tgcctctggt ggataaacgt gggcgatttg ttagtgaggt aactgatttt tccggtgaat
    17521 ttgtgaaaga agaatacctt tcggaagagg agaaagaaca agaacggcaa aaacaaaaca
    17581 gagataaata cttgtcggta gatgagcgta tagccatcaa actaaaacag gaaaacaaag
    17641 cttttaaagt tgagaagtac gaacacaact acccacactg ctggcgaaca gacaagcctg
    17701 tactttacta tccgctcgac agttggttta tacgcactac tgcatgcaga gagcaaatga
    17761 tagcacttaa taagactatc aactggaaac ctgagtcaac aggaacagga aggtttggta
    17821 actggctcga aaatctggtt gactggaatc tgtcgcgttc gcgctactgg ggcatacccc
    17881 ttccgatatg gcgaagtgag gatgagaaag aagaaaaatg cattggctct attggtgaac
    17941 ttcgaaaaga aattgaaaaa gcaattgccg ctggggtgat ggatgccaaa catttagaac
    18001 atgttgatgc ttcaaaatct gactttgacc ttcaccgccc ttatgtggac aatattgttc
    18061 tgatatcaga tacaggccaa aaaatgtttc gtgaaaccga cttgatagat gtttggtttg
    18121 acagtggcgc catgccctat gcacaatggc aattagatca ggataaatta agtaaaggct
    18181 tacctatgcc gttcaatgaa ggttttaaca ccggctatcc tgccgatttt attgccgaag
    18241 gtgttgacca gacacgcggt tggtttttta ccttacatgc tattgcagtg atgctgtttg
    18301 attcagtagc ttttaaaaat gttgtgtcaa acggattggt actcgataaa aacggaaata
    18361 aaatgagcaa acgcttaggc aatgctgttg atccgtttga aacaattggt aagtatggcc
    18421 cggatgccac tcgatggtat atgataagca atgcacaacc ctgggacaac ctcaaattta
    18481 acgtagatgg cattgccgaa gtacaacgaa aatttttcgg aaccctttac aatacttatt
    18541 cgttttttgc attgtatgcc aatattgatg gttttgttcc tgatgaaaaa aataaaatcc
    18601 ccttgcatga ccggcctgaa ttggatcgct ggatactatc aaaacttaac aatttagtgc
    18661 agcgtgtcac agaaagtctg gacgattatg agcctacctt tgcagcccgt ctgatagaag
    18721 aatttgtgga tgagcatctc agcaattggt acgtacgtct ttcacgcaga cgtttttggc
    18781 gcagcgaaat gaatactgat aaacaatcgg cttatgaaac gttgtttgaa tgtctaaagg
    18841 ttacggcaca actgatgagc cctgtggcac cattcttttc tgactggctg tacagaaacc
    18901 ttaccgatag cctgcgcagc aatgccattg cagcaaaaac tcctttgcaa tattcctctg
    18961 tacaccttac ttatctgacc aaagcagaaa ctaatatgat tgatgctgca ttggaggaaa
    19021 gaatggattt tgcacagcgt atttcgagca tggtgctggc attacgcaag aaagtaaaca
    19081 tcagagtgcg gcagccatta caaaaaatac tcattcctgt ggacagcaca cagcagcaaa
    19141 aggctattga acaggtggaa aaactcattc tcacagaggt aaatgtgaaa gaaatggagt
    19201 ttatcaccga tacctcaggg ctattggtaa agaaaataaa ggccaatttt aaagtattgg
    19261 gtaaaaaact cggtgcatta atgaaagatg ccgctgctgc tattgcacaa atgactcagg
    19321 atgatatacg tatcttagaa aacaaaggtg cttttgacct cagaataaac gaaaatatag
    19381 tggttatatc tgtggaagat gtagaaatca gctcagaaga cattccaggc tggcaggtca
    19441 acagcaatgg gggtttaaca gtggccttag acatcagcat atcgccccaa ctgcgtgacg
    19501 aaggtattgc ccgtgaactg attaacagag tacaaaacct gcgtaaagac aagggatttg
    19561 aggtaacgga taaaataatg gttaaactta aaaatcagtc atccataacc actgccatcg
    19621 aaagtaattt agactatatt tgcgcggaaa ttttagcctc ttcatttaca tgggtagaaa
    19681 cattagctac acctgtagcc gatgaaatag aggttgaaga aggagttaaa acattaattg
    19741 aaatagaaag gatttaaaaa tggcaagtaa agcgaagaag aagcctgtgg ctaagaaagc
    19801 tacagcgaaa aaggctacag cgaagaaggc tacaccaaaa aataaaaaag cagcggttaa
    19861 aaaagttgtt aaagtggtaa aaaaggctgc ggctaaaagt aagccttcaa caaaaaaggc
    19921 agtagctaaa aaagttgtag taaaaggcaa acctgtcaag gcaaagcctg ctcctaaaaa
    19981 ggctgctgca aaaagcaaag caactaagag taaagtcgtt gctaaaaaag tagtaaaggc
    20041 aaaacctgtt gcaaaagcta aagtaaaggc agtaaagcct gcaaaagttg ctaaggtaaa
    20101 aacagcaaaa gttgttaaac ctgtagttgc ttctaagcca caaaaagaag ataaaaacaa
    20161 atcggcaaca tctatgatta aacacgtaga aaagccaaga gaaagttcac gcagacgcag
    20221 caaccctcca attgtaaaac ctgctgcacc tccacgcact acaccgttgc gtgaagaccc
    20281 tgttcttcct gatgctccag ttgcttctat tctaaaaaag ccggttgcaa tgctcgacaa
    20341 cagaccaaca aaattggaaa acagccccga taaaacacgc tacagtgatg cagaattaaa
    20401 tgagtttcgc gagttaatta ttcaaaaatt gactgatgca aaagttgagt tgaacaacct
    20461 gcaggcattt cttaacaaca gcaatgaaca tggcactgac gacactgcat catcatttaa
    20521 gatgcttgaa gatggcagcg acactttagc caaagaagaa gccggacagc ttgctgcacg
    20581 tcaacgcaag tttattgaac aacttgaaaa tgcattggta cgtatagaaa acaaaacgta
    20641 cggactttgt cgtgtaacag gtaagttgat acctaaagaa agactacgtg ctgtaccaca
    20701 cactacacaa agtattgaag ccaaagtaca acagtatcgc gattaatttt tatttatgaa
    20761 cgaatatgag gattgtcaga ttttttggca atcctcattt tatatcaatc tattcaattg
    20821 aaaaaatata ttacccttgt tgccgtcatt ctgattatag atcagtgttt gaagttttgg
    20881 attaaaacca atatgtatct cggacaggaa taccatattg caggcaactg gtttatcatt
    20941 cattttactg aaaataatgg aatggccttt ggcatggagt tgttcgggaa aaaatttctc
    21001 tcacttttcc gtatcggtgt aatgtttgca ttggcctggt atgtatggca tctgcataaa
    21061 acaaaagcgc atcacattta caccacagcc atggcattaa tttttgctgg tgcagtaggc
    21121 aatattattg acagtatttt ttatggccgc cttttttcct ccagcgaatt tggtgtagct
    21181 acctttatgc ctgatgccgg tggttatgcc ggttggctac atggcaaagt tgtagatatg
    21241 ttttatttcc cgattattca aacccactat ccgcaatggt ttccattctg gggtggagaa
    21301 gaatttattt ttttcagacc tgtttttaat attgccgatt cggcaatcac tgcaggtgtt
    21361 ttattgatta ttctttttaa ccgtactatt tttaagcaca ttgaaacaac tcctgaaaca
    21421 gaacagacta ctgctgcaat accttcagag gagttaaatg cctcagaaag ccataacagt
    21481 tgattttaag agaaaattta gctgaacaaa aggcatacct tttgttcagc taacattata
    21541 tttatcgctt aactaatttg tagtttgtat ttccattttc attgtttagt ttcagaatat
    21601 atatgccact aacaaattgt tgcatattca tcacattcct accctgatgt aatataccac
    21661 tccacaattg ctttccttca atagtcataa tcactgcatt ctctgtcttt ggtaattcaa
    21721 ttgtaagata gtcgttaaac ggatttggaa acacattatg tgcttgttta aacatagttt
    21781 gtaatccaac tgttgtcata ccaaacactg ctgacatagc tgaacaaccg ttagcatctg
    21841 taatcaataa agtatagtct ccgttttgtg atggaacaaa aaagtcattt gtagcaccgg
    21901 taatggcatt gccgttgtaa aaccactgat aagtacctgc acttgttgtt gccaataatg
    21961 aatcgttaga gagtacaatg gtaggaactg caggtaatgg gtatgctgtt actgtaacaa
    22021 gagttgacgt ggcagaaccg caaacattag ttacttcaac ctcataattg ccgctctgtg
    22081 ttgctgttat taattgtgaa gttgccaaag gtgttccatt tagattccat tgccaggtgt
    22141 aattattacc tgatggtgct gtaagcacaa catctcctcc tgcacagaat gacaaagccc
    22201 ctgaagcggt tattactgca attggtaaac tgtctgctgt cagtattaca gcagcagaag
    22261 tatcgcttcc gcataggtta ctttcaacaa tattgtaaac accggcagta tttgcaaaat
    22321 gattattgcc ggaaataccg ggaagcatat ttccattata caaccatgta atacctgcag
    22381 actgcaaaga atagctaata agtacagaat caccatgaca taaagttgaa gaagctgctg
    22441 tgagacttcc tgcaatcggt gctgattgaa cggaaactgt ttgaacagat gatgttacat
    22501 taccacaact gttgctcaca acaacagtgt aatcacctcc attagcagca gtgtagtttt
    22561 ctgttgtagc accggaaata tcaacaccat tcaattgcca ttgataggaa tatgttgaat
    22621 caaataaagt agaaagtgta actgcattac cttgacagac agataacgct cctgaagcag
    22681 tcaccagaac tgtgggtaat ggcataacat taatcgaaac agcatttgat gcaacagtac
    22741 cacatgagtt agtaatattt aatacatagt tacctgttgc tgatgccggg tagctatctg
    22801 tagtagcacc tgaaatgtct gtgccattta gttgccattg ataagtgtaa cctgaatcac
    22861 tgtttactga ctgaagtaat gcagagttgc cactgcagaa agctgataaa ccctgattaa
    22921 caatagaagc aactgcactg ttctctattc ttaaatcaaa tccattatca cctcctgtca
    22981 aagcaggact tgaagataca attctgattc tgtaaccatt ccctgcaggt gtattggcag
    23041 gaattactgc actaatattt ccagaaactc ttgaagtaac atttccaata tttacagcat
    23101 ttgcaaagct gcctgtggca tcgcttaatt gtgcagtaaa agaattagca gactgtaaaa
    23161 atccggaacc gttgaaacta cctgtagctg tgtatggaat tgatatgcta ctacccgcac
    23221 agtatgctgc atttaaactt ccgggtgata ccgtattata atcatcacga tataaaattt
    23281 ccgtcactac ttctaaccct aaaatttctg ttgttgtaat ctgtagtaca ggaataattt
    23341 cattatttgc caaccacttg tattgtgtag tcttaggtct gtctaaagca aaatgcaggt
    23401 ttaatgtgtc aacgcttata gtgtctttaa tatacaattg tgtcttaact cgtaatgtat
    23461 tgaaagttcc atgtggtgtt gtaagcattc cccaaccatc aacatgatta attcttgtct
    23521 gcgaaagtcc ataataacct aaacccggaa gtccaatatt ccatgctgac tgagaagtgt
    23581 ctgcatcgtt aaagtttaat ggaaggttgt aaataatatc atgctgactg aaggcaaccg
    23641 gcataggaat atttgcaatc tctgcaccaa agcccacctg tttatataca ttacttgagc
    23701 gataataaaa attaaatgga tcagtaatgg tcaacaaagg gtttgaaggt aaagcttgtc
    23761 cgggctctgt tacattagct ctgttaggat taaatggcag attagaaaaa aacaaagaat
    23821 aaataaatcc tgtagaggaa acactctgat aagattttaa atcttgccca ttattacgga
    23881 ggttaccaaa actccaggtg tgattggcgc ctgttgcact aaagtttatc aatggattta
    23941 ctacagcttt tgtaaatcgt gcagtatcgc cagccgaagg catttcattg tttgtaatac
    24001 taatttgagc ctgtgaacat aggcttccta ataaaataac agcagtagat agtaatctca
    24061 taatactcgg attttgaatt atacccataa tatctcaata tccatgcaag taatgcatgt
    24121 tttgggtaaa ggtgtgatat aaccttgtga gcggctgaaa ttttcaaact aaaaaaaact
    24181 ctcttataaa aagagcataa acatttttat ctgctttact aaccaggaat attagccgtt
    24241 aagccaaaat attatcagct attgaaataa gaatacataa agacaattta gggcatccat
    24301 aaagaagtac ctcttattta gagcttattc aggaacagaa tgcatacaaa ggtttagaat
    24361 aaaaacaata atatattact atgccccgtg ttctttcaca atcttattaa gccattttaa
    24421 catcagtaaa ataattactg tagttatcat cactaacaca aaattaatta agaaaaagtt
    24481 tgctttatgt tcgtattcat cccacatgct tgccaacaca ccactcaact tatttcctat
    24541 tgatgtagat aaaaaccatc cgcccatcat cagtgctgta agacgtggtg gacttagctt
    24601 tgaaaccaat gacaatgcca ttgggcttaa acaaagttct ccaacagtaa ttaccccata
    24661 gcttgcaata agccaccaca ttgaactttt ttctaaacca ttatgacatg cataagtggc
    24721 tgcaaccatt accaatgttg acaatgatgt aatgagaaaa ccataaaata ttttccaggt
    24781 agttgaaggt tcgctgtttt ttcttttgag aaatgcccag aatgcaacta tcagaggtgt
    24841 taatactaca acccaaaatg gattaattga ctgacctaat tccgttgaga taagtgttag
    24901 cgactcccct tctttaggta atttatctac aggaatattc ttgtaataaa gtgggtaatc
    24961 ccatgtttta attggttttt tattctcgtc acgtgccaca cgaaactggt tgtctgttaa
    25021 tggcacagaa tcttttgcat aggttactgt ttgtgttact ccaagcattt ttgcaacagg
    25081 ctctactcgc tgtggcactt cgcgattagt atagctttcg gcccatgtag tgagtgctgt
    25141 tccgttttgt ttaaatatag cccaaaaaac aattgcaaca gtaaacactg ccagtaatgc
    25201 cgctaacgga cgtctgtctt ctttgtcgga acgcaaaaga atatttacat aatatccggc
    25261 aacaggtagt gcacctaaaa taaatgcatc ggttgaatcg ctaccgaaaa tattatcggg
    25321 aattatccag ccaatataac ctgcaattaa tgccggtaat aatgttatgc ccaatatttt
    25381 tgaaacaggc atatcttctg gttttacagg cttgcgaaca tcggcatgtg catagtgctt
    25441 attgccaatc caaaaaataa taacccctac aaacattcca atgcctgcag cagcaaaggc
    25501 agcaccccaa ctgaagttgt ttcgcaaata ggagccgaaa aaattacaga taaatgctcc
    25561 gatgtttatc cccatgtaaa aaatattgta gcctgaatct ttaagtggct tatattccgg
    25621 atcgttatat acattaccaa gaatggttga aatatttggt ttaaaaaatc cattgccaat
    25681 aataattagc aataatgaaa cataaaatgc tgtcataccc ggtatagcca aaccacaata
    25741 tcctatgccc ataagaatac cgccaagtgt aatggaaact cgatatccta aaactctatc
    25801 agcaattaat ccaccaacaa acggagtaag gtaaactaat gcaatgaatg ttccgaaaat
    25861 atctgaagct tcttttctgt ccattgccat accaccacgt tgtgtgtctg tcatgtaaag
    25921 gaaaaaaata ccaagcatga gatagaaacc gaatcgttcc cacatttcgg acaggaacag
    25981 gtagggcaat gcctttggat gcttcttatt cattttgaaa cgatttaaaa tttcagcaat
    26041 gataaaaaac taaactaaaa aaacaatatt ttttttaggt gtgcaataaa ttttttacgc
    26101 ttttaaaaat atcagatgca acttgctgaa cctcattttt accattaaca aaaacaatgt
    26161 tttctgattc ttttagtaat tcgaatgctt taagataatt gctataaacc ttacgaaggt
    26221 tttcattggt ttcgtacaat tctgttgagg tgcgtgtaga aacaattcgc ctaaatgcag
    26281 tttcaggatc tacatctaca aaaatattcc ggtcaggcct aagcaacgct gcacatttag
    26341 cattgataga cattacccaa tccatttcta cataggcact atgataggca taggacgaaa
    26401 aataatagcg atctgtgata accgtatagc ctttttcaag cattgccaga attccatgct
    26461 ctttatcaag caaatgttgc aatctgtctg caacaaacaa tgctgcaatg gtatgctgat
    26521 ctgcatttac tttatgatta aaaattgaac ggatgagttt accggcttcg aaacttgttg
    26581 gttcatgcgt tgtatatact ttatgaccca cttgttcaag gttggttttt agtaattgca
    26641 cctgtgtact cttaccacta ccgtcaattc cctctatcgc tataaacaaa ttttcttttt
    26701 tcatgaatga cagattcaaa cagaataatt aattcagttt ggccatttca cttttgaggt
    26761 agtcaataac tttcacttct gtacaatcaa tatttctgat ttcactaaga taccagcttg
    26821 tccagtcacc gaagtgaatc aaataaattg ctttttctat tggtgaacca cctttactcc
    26881 atacttcgta aatgtgcggt gtgtagcgac caaatatttc tttacaaata tccattcgct
    26941 ttgacgttct gaaaaaatca tctttatctc tgaacaacac tactgcaata tcattgtgtg
    27001 gctgtgtcca ccctactaat tcattatgtg ttaattcggg aaacacatga tgccaacata
    27061 acatttttcc attctcctgc acttgttgcc tgaaacgaat aacaacgcct tcattgttag
    27121 atgcagaata aattacagga atttttccaa tcaaatctga tgcaagtttg tgcgaggcag
    27181 ttttaatatg ctgcgattca gattctataa gctctattgc cttttttaag gatgatttaa
    27241 aagtatcacc aatgagctta aaagcatgaa gtatataaaa aagttgtgtt gatgaataac
    27301 catagcagga acgtggcggc ataccacccg gaattaatat ataatctaac ccatgttgtt
    27361 tagctaattc aactgtttta cctcctgagc tgacacaaac cacttttgca ccttgtttta
    27421 ttgcattatc catggcctga agtgtctctt ctgtatttcc tgagtaagaa cttactacta
    27481 ccagcgaatg tttatttaca aacgaaggca taaaataatc tttgtttact aaaaagggaa
    27541 tgggcatctc tttttcacac aactgcgata cgatggttcc accaattccg gatccaccaa
    27601 gtccggttac caatacattt tttatttcat gtggccagct tgtagcttta aaagtttcgc
    27661 caatagtcat ggcctcacgc atttgagccg gaaatttttc gattagttgc ttcatatcca
    27721 tacagcagaa aaacttaaaa tgttatgctg caaacataag ctatttgcac gaaaatttat
    27781 acttattaaa gtaatttcag ccttgccttt tcttctcttt aatccattgg ttaatggatt
    27841 tttcgagtac acttagcgga agacaaccat aggaaagcag ctgatcatga aattgctgta
    27901 aactgaattt gtcttttaat tgctcttcag ctttttgacg taattcaata atttttaact
    27961 gacctatttt atacgacaat gcctgacccg gaatggccat atagcgttca atttctgcag
    28021 taatatctgc ctcactttcc atttcattat ctaatgaata ttgaattgct ttttcacggg
    28081 tccatccttt aagatgaatg cctacatcta caaccaaacg aatggctctg tgcatttctt
    28141 cactcaggca accaaaatac tgatagggat ctgtatataa ccccaactct tttccaagtg
    28201 actctgtata taatgcccat ccctcaccat aggctccaaa ccatccaaag cgtctgaact
    28261 caggtagtga tgtgttttcc tgttgtaatg aaatctgata atgatgaccg ggaatagctt
    28321 cgtgcaaaaa caaatcttcc atacctacta cattatattt tgacgcatcc aaaataggta
    28381 cataaaaaac tccaggtctt gaaccatcgg gtgcagctgt attatactca gcacttgcag
    28441 atgctgcacg ataggcctca gtttgcctaa cttcaaaagc tgctttcggt acatgattaa
    28501 acagtttttt cagctgtggt ttcattctgt cctcaatagc aagataggct gcaattacat
    28561 cagaatcatt tttaaatgga aaaaattgtg gatctgtatg aatgaaatga aaaaattctt
    28621 taagtgttcc tttaaatcct gtttctgcct ttactttatc catctccgta tgtaaacgct
    28681 cgacttcttt aagacctaca ttataaattt catcgggagt cattgttgtt gttgtccatt
    28741 tatttgcaag ccagttataa tattcttttc ctcccggaat attagaaata cctgcaaccg
    28801 gaagacaagc cggcaaatat tctttttcta aaaaaactga catcttttta aaacttggat
    28861 ttaactcctg caatatagct ttggaataag caattgttaa tcgttcttta tccgtatcag
    28921 aaaaactatc aggcatattt ttaattggtc cgtaaaaaac actttcttca actttacttg
    28981 caatgagtga tttatactga gggattgttc tttctatgag gatacgtgcc gggactatgc
    29041 cttgcaacat tcctcttttc atattaccta tggaagtatc ctgataagct acaaaggctg
    29101 taatacgttt cagaaaatta tcgtagtcct ttacagtttt aaatggctga gagctggtgc
    29161 cggaacctaa ttgagaaaat gtcagatgca taccccaaaa ttgatttacc ggcatcaggt
    29221 ttgaaggaaa ctttaggcct tcttcctcag tgtttaaaat atactgtaac acttcataac
    29281 ttattaagtc ctgacccgtc aattcaggct tgttaatggt aagtagtttt tcggaataac
    29341 tgtgatagaa ttgacgaagt gtatctatat aggcatcgct gatatcaatc ggcagcaaat
    29401 cattatacct attataggca tttgccgtag cctcaagagg gaaatagcgc attcgctcct
    29461 gatagtagtc atcagtaatt tcttttaaca gtacagatgt gtcttttatt gactgttttc
    29521 tattctcaga acatgagata ataaaaagcg cacaagctat aaatagatat tttttcattt
    29581 tcactccaca tagaaaatcc aaatttaaaa ttaataaata tttcttgata tagaattata
    29641 tcgagaattt ttgatcaaaa ttgcagataa accggcatga caggatcggc ctgttttaga
    29701 acgattacga taattatcac tgctgtagca actgatgctt tgactaacca atgccacttt
    29761 tcagagtagt tgataataat ttcattccat cgaattggta aaaaatgcaa tacaaatcca
    29821 agtaacatta ctaacaatac ttcactgtaa ttagtactga atagtgtcca accttcagca
    29881 taaaaattaa acacaatctg atggatcatg ttaaaactgt cggtaaaggt tgtgcattta
    29941 aaaaatatcc agcaaagaca aacaaaatgg aatgttataa agattcctgc aattcgtgtt
    30001 aaacgccccg aaaaatgtga aggatatgct gccttccaca acttatgaaa agctaatgct
    30061 acaccatgta atgctcccca aaaaataaaa ttcaaactgg caccatgcca aagtcctcca
    30121 agcaacatgg tgataaataa attcaggtag gttctcactt cgcctcttcg attaccacct
    30181 aatggaatat aaagataatc gcgcaaccat gacgaaagtg atatgtgcca tctgcgccag
    30241 aattctgtaa tagaagctga ttggtatggt gcaagaaagt taatattaat atcaatacca
    30301 atccattttg cgattcctat tgccatatca ctgtagccgg aaaagtcaca atagattacc
    30361 attgcatagc catacacagc aaaaagacat tccatgcctg tgtgccgcaa agggtcatca
    30421 aacacatatt gaaccaaatt gagataaata aaatctgaaa taactacctt cttaaataat
    30481 ccggttagaa ttaaaaataa acctttagag acatcatgtg ctgtgagtgt aatctccttc
    30541 cttatctgag gaatgaaatc tgcagcacgc actattggtc ccatcatcag ttttggaaaa
    30601 aatgaaagaa aaaacaggta gtcaataaat cgttttaccg gcacaaactg tcttctgtaa
    30661 acatcaatag tataactcag attttcaaaa gtataaaacg atattcctat tggcaggtaa
    30721 acatctgccg gctgaacatt accggttagt acagcattat acaaatcaat tagcaagttg
    30781 gtatatttga aataagctaa tagcccaaga ttgatgacaa cactgaaaac gagcaatgct
    30841 tttctccgaa aatccgttgt tgcattgtga attaaattac tgaggttaaa atcaacaatg
    30901 gctgccacaa gtaccaacag cacatatact cccgaagctt tataaaagaa ataaagtgag
    30961 aaaagtgtta ctgcaacaat acgcaaagtg cctgtccgtt gcagaattct aaacacaaac
    31021 aaaaacaaca ataaataaaa aagaaaaaac gaactgttga aaagcaatgg atttgctttg
    31081 tcatacaaaa actgatgtcc aagtttttcc cagtctattg tcattgctca gttaaaggtt
    31141 taagtagtgc ctgaagcaac attaatccct gcatttcata ccccgaacgg ttcaagtgaa
    31201 ttcgtctggc atcactcatt ttactttttg caaaacgaag catacttccc ttaccacctg
    31261 agtactcata aagatcaaaa aaagctattt tattttctat acaatacttc tgaaggagat
    31321 ttctgattgc aattacattt ggattaggta cgtatacacc acgtctgata cgtttgcacg
    31381 actcaggtgg tgaagtaatc aaaattgaag ctgatggtga aacattcctg agtttggtaa
    31441 taaatgaatc aactgtctga ataaatatta ttccatcaaa attctttgca aaagcctcgt
    31501 tagtgccaaa tgaaagaata tacaggtcag catccagtgt ttttacttgt tcaaagaaca
    31561 atggttgtct gagaaagcta ttgtactctg caccatttac accaatagta tgatagataa
    31621 caccggagct atcattcaaa agatttaaac caaataatgt aaaagcagta tctgaattga
    31681 ttttaatttt acaattagtt aaataattag aaaatctgaa ctcattaatt tctgctgaaa
    31741 gttttattgg gtttactgtt tccattattt cagaactatc acttgcagtc atgacagcac
    31801 tgtccggttt gtaaattacc tgaactttat taaatctata atctatacct tcattatttc
    31861 gtaaactaat tcctacagat ggattactgt cgttagttgt tactgaaaat ccggtgatgc
    31921 ccgttgataa agaatcttga gtaactacat ttctatttcc tttccagtta ttctgagaaa
    31981 aagaaacata atcatgtggg ccattagaac gcgcaagtct ataaggaaaa acaactcctc
    32041 taccggcatt accatagcgt tgttgtagtc ctctgcgtac catgccggaa aaatatccag
    32101 cttgcaaatg agagtcgcct atatgcacta tactaatttt ctttccggca gttttacttt
    32161 gtaacaattt gctgtaaaaa ttcttcaatg cttcctgatg ttcaatatgc gctgcatcag
    32221 gattgaggaa ttttaataaa gacattttat acgcaacaaa cttttcatat tgcaacacag
    32281 gcattatagt atcggattcg ctttgtgcat gacaccttgt acaatatatt aataaagata
    32341 aaattaaaat tattctcttc attgttttac ataaggatta atcattgtat cttttttcac
    32401 gtccataact tccacagttt tactatatct atcgtagctt ttcacaatat catcaaacaa
    32461 cattccggca atttttcttc ctcctgcaaa attagggtgt gtgtaatcta aatttgctaa
    32521 tcgtggatta ccctctgccc aacccaccat actgttataa ccacccattg cttcatataa
    32581 attataaaaa gccacaccac ttctttgtgc aatgcgtttt tgtaattcaa caaaaattgg
    32641 aatgtcaggc tcggtttcat aaccatcttc tgcacgatag cttttatctc ctacactcat
    32701 aattaatatt gatgcttcag gaaatgcttc tttaatgtag tttaatgttt tctgaaatga
    32761 tgatgcatac caactgtaat cttttacatc atgtgcaaca acatttaatc cataacttag
    32821 aattatcagt ttgtaattct cttttgtcgc aagagtttgt aattctccaa aaggaatatt
    32881 tgaaagagga atgcctgaat ttcctcgaaa tgaaaaatta tctacataaa cgccattgcc
    32941 attgtcgaag tagagtccat gaacttcaca accgggtttt tcaaaagtta attttacttc
    33001 ttttataggc ttgtctttaa ccaattcaat ttctttaaat ccgttaatac tatcagttgg
    33061 aaaaatatga gcagtgtcat cattcagaat aaaacttgct gatgggcact gacgacaaat
    33121 catttttact tctgaataaa tcggataaaa tctgcttgca ttgtaatgaa cccaactacc
    33181 gtctgacgaa acaaagctgt atcctcctat tcccagttta taattctgtt ttggtttttt
    33241 cataaaacta taaacctccc agtcgtcaga aaaggtttgt tttattgttt gcctgaattg
    33301 tgccaccggt gaagtaacag gaacaaagcc aacaccacta ccaccaaatt ttttctgtat
    33361 caactttcgg aaatcctgcg ttaccaaatc accttcaatc atcgaatcac caaaccatgc
    33421 aatcctgatt ttttcattct tatttgcatg attgtgtaat gcttcaaaga acttagttaa
    33481 atcaagttcg cccaccttat aatttatttt aattgcagtc gctctaactg tgtctgttac
    33541 tatctgttta acagtatcat ttttaaaaaa aactgaatca tttacaatcg ctaaatcttg
    33601 tgatttttcc ttcttatttt ctttctgaat aatatcacta tagaggttaa tagttttact
    33661 ccctgtgaat ttttttggca ataacgatat tacagttaat atgattacca gaaaaaacag
    33721 aatttttaca ggctcgttat tgcggaaatt tttcacagct tatgattgac tttactgagc
    33781 agttcgtggt ttaacctgtt ttaaaacctt aatgtagtgt ggagatcgcg cataccttat
    33841 tcgttttaga aaatcataat agttttctga ctggttggta taaaatttat cctgccactt
    33901 tttataatag tcaacacttg cttgccaatt ggcaaaactc atatagcgct tggtaaatcg
    33961 aaatgcaaac aaattatttt tatccataag gagtttggct ttgaagtgtc ctgtttcaag
    34021 taaagcttgt ttcaatacta tatcagaatt taaaacccca atactatcca tataactttt
    34081 aacatcatca acatttactc tcggctgctt ataatacttc tcctttctat gtttattgac
    34141 aacaactttc gcagagtcag aaaaagcaaa cagattatcg ctattgaata ttaaagcaat
    34201 caaaaaaaga aaagtgtatt gtctaaccaa tctgaaagaa gacttcattg ttgcaaaatt
    34261 atcaatttaa acatgtttaa taaccgaatt taggccctaa gtaccaacta tgaaaaagta
    34321 attaaaagca gatgagccac tttacaaatt gcaaagtggc tcatctgaaa taattattaa
    34381 ttcacattac tcttcacccg gaaggttaga gttttttgtt tgagaatacg gaactgtttg
    34441 tggaatataa tcatcatctg ctccaggttt gctatagtca taaggccaac gataaactgt
    34501 tggtatagga ccattccagt ttccatgaat accaggacct gccggagatg tccactcaag
    34561 tgtattacta tgccatggat tttgcggtgc tttctttcct ttgaatattg aataaacaaa
    34621 attgaaaagg aaaatcacct gtgcaattgc tgcaagaaaa gcaaatacag ttattaaggt
    34681 attaatattt aaaaacacat caaagccttc gtaagcagaa ttggaataat atcttcttgg
    34741 cacaccaccc aaaccaataa agtgcattgg gaaaaacacg ccataaactg caattagtgt
    34801 taaccagaaa tgcacataac ctaaggtctt attcatcatg cgtccaaaca tgcggggaaa
    34861 ccaatgataa actcctgcta acataccaaa tattgcagag gcacccatta caacgtggaa
    34921 gtgagcaact acaaagtatg tatcatgaac gttgatgtct aatgcagaat caccaaggat
    34981 aattccggtc aatccacctg aaatgaataa cgacaccaat ccaattgcaa aacacatggc
    35041 cggtgtgagt tgtatttttg ctctccataa agtagccaga taattaaaca ctttaaccgc
    35101 tgaaggaatt gcaacaagta acgtagtaaa tgtaaacacc gctccaagaa aaggattcat
    35161 acctgttaca aacatgtgat gtccccaaac aatacatgat aaaaatgcaa ttgccattaa
    35221 tgaaccaacc atggctttat atccaaatat tggtttacgt cccatggttg acaacacttc
    35281 agatgtcaaa ccaaatgctg gaagaatgat aatatatacc tcagggtgac ctaagaacca
    35341 gaacaagtgc tcataaagca cagggcttcc acctgattgt ggcaatgcta cgccaccaat
    35401 aaaaatatct gaaagataaa aacttgttcc aaaacttctg tcgaatacca aaagtaatgc
    35461 tgctgcaaaa agaaccggga atgtcaatac accaagaatt gcagtaataa agaatgccca
    35521 aacagttaaa ggaagtctgg tcatgctcat tccttttgtg cgaagattga taacagtaca
    35581 gatataatta atacctccaa gtaacgactg gataataaag aataccatac tcaccaacca
    35641 cagcgtcata ccaagtcctg aacccggcat agcttgcggt aatgcactta acggtggata
    35701 tactgtccat cctcctgctg ctggacctgt ttcaatataa actgagtatg tcatcactgc
    35761 acatgaaagg aagaaaaacc agaagcttaa catatttaaa aaacccgatg ccatatcacg
    35821 tgcaccaact tgtaatggaa taagcaagtt gctgaaagtt ccgctcaatc cacctgtcaa
    35881 tacaaagaac accatgatag ttccgtggat agttacaagt gccatataaa atccgggatc
    35941 caatctgcca cctttacccc attttccaat cagactttcc agaaaagcaa attttgtttc
    36001 aggaaatcct aattgcaaac gaaaaattaa agacatatac attccgaaga atgccatgat
    36061 aattgccgta attaaatatt gcttagcaat ggttttatga tcctgactga aaatatactt
    36121 tgtcagaaat gtttcgtgat gatcgtgctc atgtgtgtga tcatcgtgat gctctactgc
    36181 aacatctgct gtttgtttta cttccattgt aattttattc ttagcctaaa ttatatcgaa
    36241 caaaagaaaa tatcaaattt aaattgaatt actgccattt gcagtactat ttaatgtact
    36301 caccggaggt gctgctttca cttcagaagc ttttttctgt gatttcaacc atgctttaaa
    36361 atctgcaggt gtgtcaacta ctactttcat tttcattgtg taatgtgcta caccacaaat
    36421 tttgttacaa agcaatacat agtcaaattt atcattaccg gtaatcttgc gcatttcagc
    36481 tgtggtaatt gttggtttaa atgagaaaaa tgtattcata cccggaacca agttcatttg
    36541 tgctctgaag tgaggaaaat atgcgctgtg aattacatca cgggcattga attgaaattt
    36601 taactcttga ccaagaggaa tatgcatttc atttcctgtt gtatatatat cgtcttttac
    36661 tgccggatca cttgcatcta aacctaatgg attatcatca gtaatgagtt tatagttgga
    36721 ttttcccagt atattatccg gtccactata acgggtaatc catgcaaact gatagccata
    36781 aacctgaaca ttcatactgt tttctttttc agaaaatatg ccattccatt gtttcagacc
    36841 ggtaatgatt aaggtagtta atacaattgt tggaacaatt gtccagatta attccagttt
    36901 ataattttca ggataaaagt acgcctcatg ttttttattg tcacgatact tgtatgaaaa
    36961 gtaaaataga gctatctgtg taataaagaa tacaacacca atgattgccc aattaatatt
    37021 taaaagttta tccaatccaa caccatgttc agaagcagac acaggaagca ggtgtttgga
    37081 atagtctatg gtcatataaa taaataaacc cagacctaat accataaata caataaatcc
    37141 gaatccattg gaattattgt gcctaacctg ttcttcttga gagtcgctgc taccggttag
    37201 ttcagaggat aattgtacaa tagtcattag tctggctaat acaataatgc ctagcagaat
    37261 agttattgtg attaaaagtg tcatgtcttg atattacaga tttgtgttaa agtctttaat
    37321 taaattacaa tataaaatgc tcgctctcct gtagcattgg atgtttttga ggaaggagtg
    37381 ataatttggt aagtgctgta aatacagtaa agataaacat gccaataaac cctatggttg
    37441 ttcctatttc caatactcct attgtacctt tttcatgcat aattccggga actaccatca
    37501 gataggtgtc aatccagtga ccaataaaaa ttacagagcc taatattgcc aataaacgta
    37561 attttctttt tgcatcacga gtcattaaaa ctacaaatgg aagaataaag ttaactaaga
    37621 aattggttac aaaaagtgtt ttatagtaat cttgtctttc cataaaatag gttacttctt
    37681 caggaaggtt agcataccaa atgagcatga actgtgcaaa ccataaatat gtccagaata
    37741 tactgaaggc aaaaataaac ttacctacat catggaagtg gttttcgttt aagtgatgca
    37801 gatatccctg actgcgtaga taaaccatta tcaaagcaat tacacctaat gaactcacaa
    37861 aaattcctga aaaagtgtac cagccatata atgtactaaa ccaatgtgag tctattgaca
    37921 ttacaaaatc ccaggcagag gtagatgatg taacggcaaa aagtacaaga aaaataatgc
    37981 tgagtttacg actgcggtta tgatgttcta ttgcacctgt taaatcttct ttcattgcat
    38041 gcttacgcaa taaaactgca aaaccatacc atattacaaa gtaggctacc attcgaatta
    38101 aaaagaatgg aacattaagg taggcacttt ttcctgctaa aatttcatca tagtccggat
    38161 tgggttttcc atcaggcaaa gtttccatca ctaactcatg gtgtgtccaa tgccatatat
    38221 gatgaccggc agcaaaaaat aaaatgagca taatgacacc gctaatccac agatattgtg
    38281 acatcgccaa tggaattcta atgatgtaaa cactccaacc tacttctgct gcatattgta
    38341 ctgcataaaa aaacagccca cctaatgcta tggttaaaaa gtaaaagttg ttgtgcatga
    38401 ggtttgccca tgtctgatcc ggattggtag caaagcttat tccgattgaa attgcaccta
    38461 tcgccatgag aataaacgaa agggttttca gcttacttga aaaggtatat gtgagattgt
    38521 cctgcatagt attatcttaa ataggctgcg ttatgttatt tattttgtgc agtagccgaa
    38581 ctatcagcta caactgcgcc tggttcgtct ccctgtaaat gattaatata taaaataatt
    38641 ttccaacgat cgtcagcact tacctgagct gcatgcgatc ccataaggtt tttaccatag
    38701 gtaatagaaa aaaacatttt cccatcaggt aggtctttaa ttgctgcact tgtataagaa
    38761 ggtacaggaa atggatctcc tttaatttta agccatcctt taccatcacc tttttctcca
    38821 tggcagtgaa tacaataggt atcaaattct gttttaccac gctccatatt ttctactgta
    38881 ggttgaaaag ggttcttcaa ctccaagcca gcagcctcat atccttcgtt agtattggga
    38941 tagataaagt caacgtactg acctcttgca actgttccgg caacgggtgc taatgcagaa
    39001 acactatctt ttgtgatgtg tgatggagaa taagctacca ctgctggagt acgatacata
    39061 tcaggcatat actccaaacc gggtgattgt ttatcaccac aggcaccaag caatagcata
    39121 ctgctaacaa ttacacttaa atattggtat gtttttttca tccgtttaaa ttctttatga
    39181 tcttatttcc actgcaccca cttgtttcag tgattcgcga attgcagttt catcacttac
    39241 ttttccttca acagttaata caaaatgatc gtcatgcatt ctatcatgaa taacatttgg
    39301 agtaacgcca ggtaataatc tacttcgcag gtaaaaagta atcaccatgc catgtgctgc
    39361 acataaaact gtaaactcga aagttatggg aataaatgca ggaatatttt tatataagtt
    39421 ccaacttggt ttaccaccaa tatccattgg ccaatcccaa atattcatgt accacatcat
    39481 taaaagtgct aaacatgttc cggttgcacc atataaaaat gaagcaatgg aaattcttgt
    39541 gcgtttaaga cctaaggcac catcaagacc atgtacagga aaaggcgaat aggcattggt
    39601 acatttaata ccttttgact ttagttcttt aatggcattt aaaagaactt cgtcatcatt
    39661 aaaaatgcca tgcaattgtt tagtcatgat gttgtactcc tttctttgat tgagagccag
    39721 aagacttcaa aatagttttt aattcgctca ttgctataac cgggaaatat cgtgtaaaga
    39781 gtaagaaggg aacaaagaac aaacctatcg agccgataaa aagacctatc tctacccagg
    39841 ttggatgaaa cattgtccaa cttgaaggaa caaagtctct gtgtaatgaa gttacgataa
    39901 ttacaaaacg ctcgaaccac attcctatgt ttacaataat tgataagaag aaagttacaa
    39961 aaaggtttct tcttagtttg ctactccaca ttaattgtgg agaaattacg ttgcaagtca
    40021 tcatactcca gtaagcccac cagtagggtc ctgttgcacg attaataaat gcatattgtt
    40081 caaattctac acctgaatac catgcaataa aaaactctgt taagtaagca caacctacaa
    40141 tagaccctgt gataagaata atcctattca tcatgtcaat atgataaatc gtaatgtagt
    40201 cctctaagtt taatacttta cgtgtaatta tcagtagtgt ttgtaccata gcaaatcccg
    40261 aaaaaatagc tccggcaacg aaatacggtg gaaaaattgt tgtatgccat cccggaacca
    40321 atgctgttgc aaagtccatt gatactacag agtgtaccga aagaaccagt ggtgttgaaa
    40381 tacctgcaag tactaaagat acttcttcaa atcgttgcca gtttttagca gaacccaccc
    40441 atcccatact caaagctgaa taccagaatt tacgggcttt ggtttttgca cgatcacgaa
    40501 cggctgcaaa gtcagggatc aagcctgtgt accagaaaac taatgataca gaaaaataag
    40561 tcgagattgc aaatacgtcc catactaatg gagaattaaa gttaacccac aatgaaccaa
    40621 attggtttgc aaatggaagt ggccagtagg caacccaagg gcgacccatg tgtgctacaa
    40681 taaatgatgc agcacagata acggcaaata ttgtcattgc ttctgcagag cggttaattg
    40741 ataacctcca tttttgacga aacaataata agattgcaga aattaaagtc cctgcgtgac
    40801 caatacctac ccaccaaacg aagttggtaa tatcccaagc ccatccaact gttttattca
    40861 gaccccaaac accaagtcct gttccaactg tgtaagctac acaaataatc caccataaaa
    40921 gtgcagcaca agcaacacta aataccatcc accagtattt gttgggttta gattctatag
    40981 gtctgcaaac atcttcggtt atatcatgaa gcgttttatc cccttcgatg agtgtcggcc
    41041 taagcggtga ttctacattc gacatattgt tgaaaaattt ctattagttc gattaatttt
    41101 atttatgctt tatgactgtt attcgtttta cttgtagtgt tacgcacctt ggttaaataa
    41161 ttaattgaag gctgaacatt aagctcttca agcaaatgat acatacgttc actactgctt
    41221 tgttttacca gtcggcttga tttatcatta taatcaccaa aagtaatagc attggttggg
    41281 caagtttgtg cacaagcagt atttatagct ccatctttta ccggatgacc tgcttttttt
    41341 gcttctaact taccgtattg aatacgctga acacacattg agcatttttc cattactccg
    41401 cgtgagcgca caattacatc cgggttcaac accatttttc cataatcatt attcatgtta
    41461 tagtcaaatt gtggattatc agaatattta aaccagttaa aacgtctaac tttatagggg
    41521 cagttgttgg cacaatatcg tgttcctaca caacggttat aagccatcat attcaagcca
    41581 tcctcactat gtgttgttgc ggcaaccgga cacaccgttt cgcaaggtgc atgattacaa
    41641 tgttgacaca ttacaggctg aaatacaact tcaggattct ctgcaggctc ttccatataa
    41701 ccatacatgc tcaacttacc aattccctgt tcatgtgctt tttctttatc catgtcgctg
    41761 gtataatagc gatcaatacg aatccagtgc atttcgcggc tgcgttgtat ctcttcttta
    41821 cctactacag ggacattgtt ctcagcagta catgctacta cgcaggcacc acaaccaata
    41881 catgcattca ggtcaataga cattccccag gcattaatcg gacgatcgaa accgggatga
    41941 tctttggttg cccaaaggtc taattcttta gcggaaactt ttcctttata agaatgaaat
    42001 ttctcttctt catttccggc ttttggatct ttaatatatt cttcaagtgt ggtttccttt
    42061 acaatagcac gacccatcat ggtgtgatga gattgtgttg tggcaagagt ataatcagct
    42121 tcaactgttt tatttaaact tgctccgcta ttggtaaagt taaaagtacc ttcagaaaaa
    42181 gtcagtaaag gatatgcatt catacctact ttatttcctg ctttaccact gtttgttcta
    42241 ccgtaaccgg cagctactgc aattgtatct tttgcctggc cgggttgcaa taaaaccgga
    42301 cctttaagag taacatttcc tgatttaact tcaataatat ttccttgaac cagacccttt
    42361 tcttttgcca ttgatggtgc tatggcaaaa tagttatccc agcatatttt agaaacagga
    42421 tcaggtaatt cttgtagcca aggattattt gcatgacgtc cattacctat accagccttt
    42481 tcataaacta caatatcaag accacctgat tttctagctg ctattgcact tgccgcatca
    42541 tttacgtttc caacaaaagc aggttcgctc tccgttactt caccattcca aacaccttcc
    42601 tgtaaaactt tttcccatgc aggaattgat gccattcctg ttgctgttgc cagaaaacct
    42661 tgtctccagt tttgcatcag aaactcatga tagtccgtta ctgctgcctc actccacaac
    42721 aagaataatt cttgtgattg ttttgtatca aatagttttt gaatggttgg ctgcaccaaa
    42781 ctaaacatgt tgcgcacggg ttctgcatca ttccacgatt caagatagtg agaatccggg
    42841 catatataat cgcatttaac tgctgtttcg tcattacgat cagacagtga aatctttacc
    42901 tccactttgt ttaatgcaga tgtaaatgct gtggaatccg gatagttata caatggattt
    42961 gcattgtaaa aaataacaac acccacttca cctgcattca tttcgcgaag caattcagcc
    43021 atggcttcat catttccctg acgcacattg ctatgtctgg caatactgat agtagcacca
    43081 taattaccaa gcatgttgtt aatggctgca acaattaatt gatcattggc attattgcta
    43141 ccacaaacta ccaacgattt accttttgca ttccacagat catttgctgt gctgttgata
    43201 gctgcatctg catcggtctt gccggcactt actgtggcag cacctgcttt tgaagctact
    43261 gcattatata atgacaataa agcattgcct gtctgacttg cttttaattt atatcggtta
    43321 tcagcattac taccggttaa agaaagtgct gcttcaaact gaatgtgttt cgacatggtt
    43381 ttttttccat catctaactt tcttgtctgt gcatactggc gtgaatattc aattggtgca
    43441 atccagttat tcagaaaatc ggcaccaaag cttacaatta catcagcttt gtcgaaacta
    43501 taatttggga gcgctgcttt tccaaatgct aattgatgtg cttttattat accatattga
    43561 gacatggcat cgtaagtaac caatttagca gtaggatatt tggtggtaaa actttcaatt
    43621 gcctttttag tagttgggct aatgatggtt gaagaaacta tgcgaatttt tgcttgtttt
    43681 gtattagctg acgcaagttt gtcttttatt tctttattta ctgtatccca gcttgctgct
    43741 ttacctttag caattgggcc tttaattctt tcgctatcgt acaaatctaa aaccgaagct
    43801 tgcactcttg cattagcacc accttttgta agcggtgaca actcattgcc atcaacttta
    43861 attggacgac cttcgcgagt ttttacaacg atactggcat aatcatgacc atcaaaaaat
    43921 gtagatgcat aataattagg aattccggga acaaggttct cggggcttat caggtaagga
    43981 attgtttttc taacaggagc ttcgcatgct gccatagaag cggctgcaac accaaaacct
    44041 aaaaatttga gaaaatctct gcgtggtgtg gcatctttgc tttcagcttt tttgccaaga
    44101 acttcatcaa caggaatttc ttcaaaaaac tcattgtttt taagacgaac aaactctgca
    44161 tcatttctga gttcttctac acctttccaa tatcgttttt ccatatatta tttaaaaagt
    44221 ttgtggccga atgtttttaa tttgaatgaa cagattcagc ttttagtgtt ttgattaata
    44281 gtgacagcgg gcacaatcaa gaccgccaat tttgtcaact gtaagtttag catctttacc
    44341 atatttggct gctaaagttt tatgcacttc gtcataatag ccattaccat ccattttcac
    44401 ttctgtttcg cggtggcagt taatacacca acccatggtt aatggagaga attgcttcac
    44461 tacatccatc tcctgaacag gaccatggca ggtctgacat tcaattttac caactgttac
    44521 gtgttgtgcg tgattaaaat aggctaaagc cggtaagctg tgtaccctga tccattgaat
    44581 tggtttttgg ttagttccgt attttgaacc atcccaatcc agagctgcat aaattttctt
    44641 gatttcctta tcaccataaa ctggaccctc tttcacatat ttatgacagt tcatacaggt
    44701 gcttgcagat ggaatattag cgtgacggct tttgtctgca ccactatggc agtaaagaca
    44761 agcgatttta ttatctccgg catgtaattt atgtgaaaaa tgaatcggtt gctcaggttc
    44821 ataattagta taaacaccaa tatctttcag tgcataccaa cctttaacac taccgaaaac
    44881 aactaaaagc aataacacaa cggcaatcag cttcttattc ttactaatcc ataaactacg
    44941 tgcttctttt ggctctaaat gaggaggatg cggaatacct tctttttcgc gaactgcata
    45001 ctctaagcct ttctttattt tattcagtgt agctgctagt gctaataata ttaagaaaat
    45061 agctattacc caatataaag tagagttatc tttttcagga gtaacagttc cgggtgcagg
    45121 tgatgttggt gcagcagcta caggtgcctg ctcactttcc ttaccaatgt atgccaatac
    45181 tgcatcaatt tcagcatcag aaagattctg attaggcatg accattttat taaactcatt
    45241 gaaaacagca acagcatcct tatcaccggc ttttaccaga gcttgtgagt tctttatcca
    45301 tttatgtaac cattcggcac tgaatttttt atgcacattc ttaagtgcag gaccaattac
    45361 cacatcattc attgcatggc atgatttaca gttggcatca aaaagtgctt taccatcctg
    45421 tgctttaact cctagtgaga ttcccaatat caggagcata aaaaacaacc ccttacactt
    45481 gacaagaagg ttattagtga gcttttggtg cataaattgt ttttgttttc gtatcaattt
    45541 ataatttagt tcaaactgca ataattccgt aaaattctag tttggagcgc gcaaatttat
    45601 aacttacctt ttttaattca taaaattttc aaattaaaat ttagtctaaa taacagcctt
    45661 ttaggtattt tttgactata ttcctcaatt taaggctgtt tttacagccc gttttacata
    45721 ttatctgtaa tattgaagcc atgattaaaa ttgtaaagtg tacctgcctt attatatgtt
    45781 gctttctgtt tcaattacaa accaaggctc agaccagcga ggatagcctg actcatgtac
    45841 ttataaaaaa gcatatagca tataatattg ctaagccata tagccagggt tttcgaattc
    45901 aactttattt tgggtcgcaa cgtgacaagg cctatgaatt aaggacagag tttatgaaac
    45961 tttatccgca aacagcagcc tatgtgcttt atcagcagcc taactttaag ttgcgggtag
    46021 gcgattttcg gacacgcctt gatgcattga aaaacttaaa tgagcttcag gcctattacc
    46081 catcggcatt tatagttaag gacgatatta aattaaaagt tgacgaataa aaaaaggtac
    46141 catttctgat accttttcat tgctgtttat ttctttcttt tagttgcgca acgctatata
    46201 atctgatagc gctgtaatgc tactttcgaa actaaactga tcattaacta taaagtaatt
    46261 gcttttgtcc actgtttttg aatatgcata ttttgctaat tcaagtttgc tggactcaaa
    46321 tgtcatttgc tgcattaagc gtgcaacctg acgtgagcta aaataattgt tattgattaa
    46381 ctgttttgca actgtcatgc gggtgctttc aaaattatag ttacctaatg actgtatcat
    46441 ctgatcaaat tcgtagtcat ctacttcgca acgaagatgt tgcggataat agtgtgggtc
    46501 ttcatatacc tgaggtgcat aatcaaaagg tctgcttccg gttatacgaa atctgctgcg
    46561 gtttacctca gccatcactt ctgatgcttc aggaataaat aaataaccgg caaatactgt
    46621 ccgatagaca gggcgatgag gatgtccggt aagtcgcatc actttaacat aatggttacc
    46681 agcgcgtaat ctatctgaat aaatatttgt tgaaacatta ctaaatggtg ctctgtctat
    46741 tgatacagtc agaagactac catcaacaga tgttatggcc aatgctgact gaaaaggatg
    46801 tgctgcctgc gcaataacag ctaaacttaa aatggcgaga gtagtaaata gttttttcat
    46861 aatgaacctc actttcttat ttaactgatg aaattcaact gctgtgccaa cattacaaaa
    46921 gataaagttt ttctgtttgt gttcagaatc ctttcatctt taaaaacaat aaattatgtg
    46981 ctaataatct cagcaaacta tacactgagc gaccacataa tgaaaaaata ctcgcaaaca
    47041 aaaccaacta taaatccagc ataaagttgt gcgtgtgtat gtgcattgtg catgagtcgt
    47101 gctgttgcag ttaaaccggc taacaatagg gtcaatacaa atggtagaag aaactcgttg
    47161 cccattaaaa ctgtaagcac ataaaatgca cctgccatac ctcctaatcc aatgagatga
    47221 atacttattt tccagaaaaa attaataata aatgcaagag caatacaaca tgctgctgcc
    47281 agaataagaa ttttaataac cgatgaaacc ggaagcttca acaaaaaata ataacaagct
    47341 acataatata aaagtgtaaa aaaatagggt attgttctgc tttgtctgtc ctccatcaac
    47401 aaaccttgca caccttgcct tttaagtaat aataccgatg ataaaaaagg catcagacag
    47461 gtgaggacaa aaacaactgc taaaatcatt cgctgcaatg caggtgaaag agtataagac
    47521 agataggtgt ccgaattaaa aagtatcagt aataaataag taggtattat taaaggatgg
    47581 gtcaggtagg tgatgactga aaaaaagcgg ttgagcattt ttaaaactct ttacgtagtc
    47641 tggcaacagg aatatttagt tgctcgcgat atttggcaat agttctgcgt gctacattat
    47701 aacctttgtc ttttaaaatt tttgccagtc tgtcgtcagg taaaggtttt ttcttatctt
    47761 ctccttcaat agcatcagac agaatttttt ttacttctcg tgtactcacc tcctcgcccg
    47821 aatctgttgc cataccttct gagaaaaatg tttttatcag aaatgtgcca aatggtgttt
    47881 gtacatattt actgtttgct actcgtgaaa cggttgaaat atcaagtcct acaacatctg
    47941 caatatcttt taaaatcatt ggttttaaaa gtcgttcatc gccttccaag aaatattctt
    48001 tttgatagtt catgatagct tgcatggtgt gaagcagtgt tgcctgacgc tgtctgatgg
    48061 catcaataaa ccattttgct gaatcaattt tttgtttcag atataagata gcctcctttt
    48121 gtgaacggtc ttgtttttta ctcttggcat agccttccat catttcattg tactcgcggt
    48181 taatacgcaa atcaggtgca ttgcgcgagt tcagtgtcaa ttcaagttga cccacattat
    48241 tagttatgat aaaatcgggc acaatatgtt gaacagattt tgtttctgca gatatatcac
    48301 ctccgggacg tggattcaat tttaaaattt cattcatggc ttccttcaga tattcgtcag
    48361 gaatattcat caatctggca attttttcaa aatgcttttt tgagaactca tccatgtaat
    48421 ttttaataat ctctattgcc ctattaaccg gcttactact ggattttcgt tgaagttgaa
    48481 gcaaaagaca ttcttgcaaa ttacgagcac caacaccggg tgggtcaaat gtctgtatca
    48541 cttgtaacac cagtaaaagt tcatcggcat tagtatctaa attttgtgta aaagccaaat
    48601 cgtttacaat agaattcaaa tctctgcgta agtatccatc ttcatccaaa ctgccaatca
    48661 gatgtttggc catctgcatt tgtttatcgt tgagagcaac catacccaac tgtgccagca
    48721 atgtttcttg aaatgttact gatgagccat aaggcacttc tgtgcgctca tcatcgggcg
    48781 aagtattatt ggcagatgtt ttataatatg gaatatcctc atcagtgata taatcatcta
    48841 catcaaaatc atcatcagtt ctttctcgaa tatcatcatc agcagtgtta tcaaactcgt
    48901 cttttttatt ctcttcactt tcgttgactt ctacaccctc ttccaattca ggtacttcgt
    48961 cctcatcctg ttgtgtatct tcatccacat cttcgggagc accttcttct aaagcaggat
    49021 tgacttccat ttcctctttt atccgctgtt ctaatgcaat tgttggtaac tgaagcaatt
    49081 tcatcagctg tatttgctga ggtgaaagtt tctgtaaaag tttttgtgat aagctttgtt
    49141 ttaacataat tcaataacgc tccaaattta ccatttttta tacgaggata agccaaaatg
    49201 gtttttatgc atttatcttg aattaaaaat tgatttttag cagaatatct tcttgcataa
    49261 gacaggtatt ttgttttaca tttgtagtct gataaagcct tctcaaatgg aaggcttcat
    49321 tgtttgatta ttatataata ttaattagta tcatgacaga ggtaaacaat gattttcatg
    49381 cagatgaaaa tcagcaggca ggtgaaactc aatcggagaa tgaatctctt gcacaaccct
    49441 tgcaggagga gcatcttgca gcattgcccg aagaacatct tgaaaccttg cctgagcagg
    49501 aagaaaatta caatcactta actaaggaag aacttgttaa agttgctgaa ggatttgcag
    49561 aaggtgatat tgctgtcgtt aaaagtaaaa tcaatgcttt gaagaatgcg tttgaacaaa
    49621 ttcaatcctc agaaaaagaa caagcgcttt ccgctttttt ggaagaaggt ggtgttaaag
    49681 atgattttaa atatagttac acacaagcag acgatcgttt tcagtcagca ctaaaaaaat
    49741 ataacaaacg caaagctgag tatgcagaac aacaggaaaa actcagagaa aacaatttta
    49801 atataaagca ggaaatactt gttgctatga agaaccttat tcaaaatgag gaaaacatgc
    49861 agcgtgcatt tgatcagttt cacgaactac aggcaaagtg gcgtgcaaca gggcctgttc
    49921 cttccggcca gttgaatgat ttgtggatga cctacaaact ttacactgac aaattttatg
    49981 aaaacctgaa aattaaccgc gaacttcagg aacttgattt taaaaagaac cttgaagcta
    50041 aaattgaact ctgcgaaaaa gcagaagatc tgattaatga accatcatta aattctgcac
    50101 tgcagacatt acatagttta caaaacaagt ggcgcgaaac cggtcatgtg ggtcgtgaaa
    50161 aaagcaatga aatttgggaa cgatttaaag ctgccagtga taaggtttat gagcgcagaa
    50221 aagaatattt tgagcaatta aaagaacgct atgctgaaaa tttagctgca aaaaatcaac
    50281 ttatcaccaa aaccgaagaa ttacttaaag acgagccatt aaaagcatct gaatggatgg
    50341 agaaaagcaa aatgcttgtt gaagttcaga atgaatggaa aaaagttggc tttgccgata
    50401 aaaaatctaa cgatgaggtt tgggcaaaat tcagaggatt gtgtgatggt ttctttgttc
    50461 gtaaaaacga ttttttcaca tcattaaaaa aagaacatac tgccaatctg caattaaaaa
    50521 acgaactgtg tattcaggca gaagcattaa tagaaaatac cgactggaaa aaaactacag
    50581 aggaattaaa aagacttcag gccgagtgga aaaaaacagg atttgccggt gaaagaaaca
    50641 atcagaaaat ctgggaaaga ttcagaaaag cctgcgattc attttttgaa agaaaaaatg
    50701 cgcattacac ttcccttgaa tcagattata aagaaaactt tgacaagaaa aatgcactta
    50761 ttgaaaaagc tgaacaattt acacttggcg aaaatattga tgagtctatt gagcaattaa
    50821 aagattttca acggcagtgg agcgagatag gtatggttcc acttgaacat aaagacacca
    50881 tttatgcaaa gtttaaagca ataattgaca aacattttca atcaataaga cagcacaaca
    50941 aagagcgatt tacatccgga cataaacaat ctttcagaac tggtaaaccg ggtaatgaaa
    51001 agaatgattt aaagcataag atgaatcaac ttaccggaga aattaatacc tgggaaaata
    51061 atcttggttt ttttgcgcgt tcaaaaaatg ctgatgctgt gcgcaaagag tttgaagata
    51121 aaatcaatca tgccaaagaa gaaataaaac gtattaaaaa acaattagac gaaatttctc
    51181 aggcacctaa agaacaatag aaattattgc ctatttccaa gacgttaaac agtcataact
    51241 tctttctctt ttgtttcgag gtgcttatca acttttgaaa taaagccatc ggttgtttgc
    51301 tgaatttttg tttctgcctc gcgagcttcg tcttcagtga tgcctttctt caattctttc
    51361 tttattgcat cattggcttc ttttctgatt gaacgcaaac ttattttagc attctcacct
    51421 tcggccttac aacgtttaac caaatctttt cttctttctt cagttaatgg tggcactgca
    51481 atacgaatga tagaaccgtc attctgcgga ttaaacccca gattggcaat ttgaatcgct
    51541 ttctcaatag gacctaacat acctttttcc catggctgga ttacaatagt gcgggcatcc
    51601 ggagtattga cattggcaac ctgatgcaat ggcgtttgcg aaccgtaata gtcaacatga
    51661 atagattcga gcatctgtgg atttgcttta cccgctctga tttttgctaa ttctgtttca
    51721 agatgcgaaa tggatttatc catatgcgac tgcatcgtat caattatctt ttttgctatg
    51781 tcgttcatac tctgtaatat ttaacagcaa aattaagcat tgaatttgaa ttgggctaat
    51841 tcaatttgaa atctacacac aaatgattta atttgcagta tgaggtttca aaaacatatt
    51901 tttgtttgta ctaatcaacg taaagaagga gaacgcaaat gttgcggtga agtacatggt
    51961 cttgcattgg ttgcagcatt taagaaaaaa attaaagaat tacagttgcc ggtaccaatt
    52021 cgtgcacaaa gatcaggttg tttagatgcc tgcgactatg gcccatcggt tgtagtttat
    52081 cctgatggaa ttttttatgg taatgttcag ctacatgatg ttgatgaaat agtagagcaa
    52141 catataaaaa acaacaaacc tgttgaaagg ctgattataa atttttaatg ataaaaaagt
    52201 gtgatttttg agtgacatta aacctcaaat cgcattcgta cttttttagg taatttttta
    52261 accacttcag catagctgtg atcaataagc cattttaatt ctgcaagtgg aatattatca
    52321 ctaaaaataa cagaattcca atattgttta ttcatgtgaa accctggtgt tatttcggaa
    52381 tagcgttcac gccattctaa aataatttca ggatccattt taaggtttac cttcattggc
    52441 ttttctgata atgaaactaa tgcaaacatt tttcctgcaa ctttaaacac aagtgtatct
    52501 gcgtcaaaag gaaaactttc tgttgcttct tgcttcgaca aacaatagtt tctgaatttt
    52561 tcaatattca tcagataata tcaaacaata aaagtagttg aatttattca catcgtacaa
    52621 aaataaaagg cggcaatctc tgccgccttt gttttaatct ttaatagggt ttgtcatttt
    52681 ccgaattaag acatcatcaa tttttatata cttcagaatt ggtttgtctc cattatttgc
    52741 ttcttcaaac ttaatcaaca gttcgttgat acgcacaatt tttctcctga agtcagaggg
    52801 ctttaaagct gttttaagaa tctttataaa tattgtggtt gactcaaact catgttcaaa
    52861 atcggcaatc tgacgtttaa tacttgaaat catttgttgt gcaaggctat cttcacccat
    52921 cagacaataa catagtccct gaaaaagttt actgtcaaca tcagtatgag taaattgctt
    52981 taaagaaata ttatttctta aatcgtaaac agtttttgct gcaccctgat agtccttagc
    53041 ataaaattta catagtgcaa tgtatcgctt aaaagagata taatgataag gctcgtttat
    53101 attgaggtca aatgtttttt caattgtctg attaaactca ttcaatctgt acatttcacc
    53161 atcagcaaga tatttttcaa ctcgcgaatt caggtactga ataataaaaa agtgcatggc
    53221 tggtttctcg ctccagtcaa taatagtgtc gtttattcca ttgctcaggt aatgatctgc
    53281 tcttacctga ttttgtattt tctgatagta ctcatagtga agtatatcca caagcggact
    53341 aatgttgcta tatagtgtgt ctatcggaaa tgcattaaaa atttcgttga tgcgttttaa
    53401 aatattttct atctcaagct caagggattt taactcctgg cgttttgatg gcaatgaaca
    53461 cagatagtag attctgacaa tattgtataa tacaaacaaa cgatgtgaat catacatttc
    53521 tgcaatattg gcaatctttc ttttgagcat tataacttct tccaatgtat ctggatcaag
    53581 tgttaattga taggctccca attttttaaa gaaaaggaaa aacatttgct cagcttttga
    53641 aatagctaat gcatatgcaa catgtttatt atagagtttg ttataatgtt cgtaatcttc
    53701 atgatgaatg tgcaacaatg caagagtttt ataaacagta attaactcat tgctcatgtc
    53761 atattcttta agctgctttt caatttcttt taatgctctg atagagaaat cgcggttggt
    53821 cccataaagc gttgcaggaa cacgaaccac ttcttccatc aactcacgaa tcggattttc
    53881 aactttctta gaaagaatct ctgcgattct tgtattgagg cgggatttta gtgtataata
    53941 agcacttgga ttaacctgaa gctgtttcat catttctgta tcatcaaaat gattgtcatt
    54001 gcgagtagct tctaggacat aatatggctt gtttttcttt ttgcctgata cctgaagtaa
    54061 caatgcctga tactcttcat cactaagttt cttaattgtt tttcttaata tatccatata
    54121 agcttttgat ttatttttta aagactcttc cttgttacgc aaaaaaaact gcaaggtttt
    54181 agctttttta aaaaaaatta acggttcatc taatcatccg attgcgcaat tcaaaccaag
    54241 tattaatcca ttgtcttcat ataatttaaa tatagttatg gataaacggc tgaaatacta
    54301 caatacaggc atacaagaaa taccctgatt aagaagtata ataatatcaa tttaaagaaa
    54361 agtgtaaccc ggatacaaaa cgggcgtaaa agccgatatg aaaagaaaat gtgcagatat
    54421 attacaatgt aatgttttat cagaaattac aaaaaccact gctcatataa aacgtaaatt
    54481 tcacttataa aaatatatca ttatggaaga tacaacgatg aacaacacaa tggactttta
    54541 caagatggtt cgtgaaaacc gctttgccat gtattcaaag ccacgcagat actataatcg
    54601 cgatggtata aaaggttcag caattaagtt cttgtttttt gtgcaggcca tcattttctt
    54661 tttgatgctt gtaacacttc attcggcaac agcacaaact gcaaatacgc aacctgcatt
    54721 aatggcagat gccggcttaa atggaactat tgcaaattac acaaccattt ataattcagg
    54781 aaaagtattt ctgaactgga cagctaaaaa cgaatcggct gattgtcttt atatcgttga
    54841 acgatcactt gatggaaaag tgtatgagtc agtaggtata aaagaaggcc ttggtactgc
    54901 tgttgaactt ttctattcat ggatggatca aacaccacca aacggatatg cattttacag
    54961 agtaaaaaag attgctcgcg atggctcgca atattacagc tctactaata cagtaataaa
    55021 tcttgattca agttatgatc cgaaatcaaa ctatgcttcg gataaaaagc cggaggatca
    55081 gaaataaaaa ataaataagg gacgacatta cactttgaat tttttaaact tatcagatat
    55141 gaacagatac tttaccacag gcagaaacat cacgtggaag ttatgttttg caattacact
    55201 tctgctgggc acttttagtg ccaaagcaca gattccaaca gcatcctttg ctgctgattc
    55261 tacagaaggt tgtgtaccct tgacggtaca tttcagtaat acatcaactt tagccacaag
    55321 ttacttctgg gattttggaa atggtaatac atcaacatta cccaatccgc aaactgttta
    55381 tttagctccc gggaactata ctgtaaagct cattgcctac aacagtactt caggaaaatc
    55441 ggatacactg atcattccaa attatattga tgttgtttca ttcccgaccg caaacttttc
    55501 atctaatgtt acatcaggtt gtagtccact atctgtaata ttcaataaca catcatcaaa
    55561 tttcacatct tgtatttggg attttggtga tggattttca tcaacacaaa ccaatccgac
    55621 acatacttat agcattccgg gtacctatac tgttaagtta gtagcttatt catcctatgg
    55681 ttgttcaaat gtaaaaacac aaacaaacta tattactgtt tatggtcaac cgacagcaca
    55741 gttaacagca aatccttcta gcacatgtga tgccaatcag gtaattcagt ttaactcatc
    55801 gtctgccggt gttgtaagtt atcaatggaa ctttggtcag ccctcttcag gtggtgcaaa
    55861 cacttctaca ctacaagatc caacacatac ttatggaact caaggttctt ataatgttag
    55921 tttgattgtg actgatgcta atggatgttc agacacggct gctataaaca atatgattac
    55981 cattggcaca gcacttacac caacatttac atggaccaat ggtgcaggat gtaagccgtt
    56041 aacaacaaat tttactgcgc catcaataac aggcgctaca agttatgcat gggattttgg
    56101 tgatccttca tcaggagctc aaaacacttc aactgttgca aacccttcac acacttatgc
    56161 aaactcagga acatactctg tttcacttta cgttacaacg tcaagtggat gtaatggcgg
    56221 tgtgatctta aataatatta ttgttgttga tcagccaccg gtggcggcat ttacatctaa
    56281 cccaacaaca ggatgtaagc ctcttgcagt gcagtttaac aatacatcaa ctggtgctgc
    56341 cacttatctt tgggaatttg gagatggtac tacttcaaca cttgtatcac catcgaaggt
    56401 ttatacaaca gcaggtactt ataatgtaac attacatgcg tttagcacaa atggttgcga
    56461 gaaaatactg gtaaagaaca actatattgt agttaatgaa attgtagcca gctattactt
    56521 ctcacccggt tccggatgtg ctccattaag tgtaaatttt atacctcaaa acaatgcagg
    56581 cgtagtttct tatgaatgga attttggtga tccggcctca ggtgcattaa acacatcaac
    56641 actatcaaat cctacacata tttataataa cgttggaaca tactcaacca atctaattgt
    56701 tacatcagcg gcagggtgta aagacacgct tataaaaaac tttgtaaaag tcagggatgc
    56761 ttctgttctt tatactgtac cggacacaat taaaggttgc caaccttatc ctgtatcatt
    56821 taccaaccca ttgccaggag ctacagtttt ccattgggat tttggtgtgc cttggctaac
    56881 caatgataca agcaatgtag ctaatccaac ttttaccttt gattctgttg gaatatttac
    56941 agtaacatta aattcattca tgccgggaaa taatggatca ggatgctcgc aattttttaa
    57001 tcctgttgcc attgttcaaa tattcccaat gctcatttca ccaatcactt atattcaagc
    57061 tacaccttgt gcaccctatc tgattcactt tagtgatacc actatggatg tagtttcatg
    57121 ggattgggac tttggtgatg gttcaccaca tgctacaact caatttcctt cgcatactta
    57181 tgcgcaaccg ggaacatata ctgtagtttt gaatattgtt ctgcaaagtg gttgtatgac
    57241 gagtttaagt acaacaatca ctttaggcta tcctaatccg ataacagtaa ataaaaaggc
    57301 cggttgtcac gatgaggcat ttacttttgg attaacagga ggtccttggt caaacataca
    57361 atggaacttt ggtgatggtg ttggtacatc aacatctgct aatccaacat atacttataa
    57421 tcaggccggg actttcacgg tttctgtaac tgtgacaagc agtgatggat gtgtcaatac
    57481 ttatactgat agtattacta caacaaatcc acagccttct tttgttatca atggaccaac
    57541 gtcaagttgt acgaatcaat ttgtattctt tacaaacaca tcaactggtg caacctctta
    57601 ttggtgggat tttggtgatc caacatcatc aacaggattt tcttcgttag aaaatccatc
    57661 aagaaatttt actacgccaa aagcgtacac catttcgcta acagcaacca atggtgcctg
    57721 ttcgcgaacg gttacctatc ctgcattaat cagaattaac agagctgcac cgaatttctc
    57781 atttacgcag agttcgcaat gtttccccat tacagttaca ctaactgatc ttaccggacc
    57841 tacaccggca attaaatggg gttggaattt ctataacgga tcaacagatt caacacaaaa
    57901 tccggtttac acattctatg ctccaccaac ctctgataca atttcactat gggttagaga
    57961 tacaaatgga tgttcagcaa caaaacgtct taaaggaata aaatacaaac aagcaaaatt
    58021 taccatgtcg gcaaccggag gttgtaatcc aacaacaatt tgttttactg acgctagtga
    58081 cacgactgtt gtttcaagaa aatgggattt tggaaatggt gctacatcaa cacaagctaa
    58141 tccttgttat acttatacat cagatggtgt atatacagtg actttgataa caaccttctc
    58201 cacaggctgt aaagacactt tagtcatgac gaatgcagtt accatttcaa cccctgctgc
    58261 tgactttagt gtgcccacac aggcattctg tgcaccagca cttgtaaatt tcaacaacct
    58321 ttctactaac gctactacat attattggga ttttggtgat ggctctaact ctattgccac
    58381 atcaccatcg catgtgtata atgttcccgg tgattatacc atcactttaa ttgccatcaa
    58441 cggaacttgt cgtgatacaa tgacaaaagt taactacctg catgtgcccg gtactttctc
    58501 ttatttcaat ctggtaagtc tgcaaagttg tgtaggcaac ttagttcagt ttactgacag
    58561 ttcattgaat gcacaaacat ggttatggaa ctttggcgat ggcgacagct ctacgctaca
    58621 aaatccaaca cacttatatt ctacaacagg aagctttaca gtttcgttga ttacaagtga
    58681 taaccttggt tgctcttcac attacatatt cagtaatccg atagttgttc atcctgttcc
    58741 aacagcatct gcaacaacta cagataccgt tggttgtagt ccgctaacat caacatttac
    58801 tcaaaactcc ataaatgcta ccacctatct ttggaatttt ggcgatccag cttcaggtgc
    58861 attaaacact gccactacat ctactgcaac gcatacctat aataatgcag gtgcttatca
    58921 gccttatttt gttgcataca ataattttgg atgcagtgat actatgtggt tacccaaaac
    58981 aataaatgta aatcaaacac cggtagcaca gtttagttca tctgcaacca atggttgtac
    59041 acctttgact gtaaacttta caaacacatc aactaaatta aacactcctc aatatttgtg
    59101 gaattttggc aatggcaata cagccattac ttcaaatgct actgtagttt atgccgattc
    59161 aggaagcttt aatgttagcc ttatagttac caatgctgca ggatgtgctg acactgcaac
    59221 tgcacaaatt acgactgttc tttcgccaac agctattgct tcaacaacag atactgttgg
    59281 ctgtagccca catactgtaa actttactaa cgtttctctg aatgcaacaa gttatcaatg
    59341 gaactttggc aatggtgcta cgtcaacact tgctaatcca agttacactt acaataatgc
    59401 aggtaactat accgtaacat taattgcaac caatgcatca ggatgtagtg atacacttgt
    59461 attcccatca cagattattg ttaaccaaac tccgagagca cgattcaata tgagtccggc
    59521 tacagggtgt tcgccactca ctgtaaactt cacaaacacc tcagcaaata cttccggtgc
    59581 aacttacagt tggaactttg gaaatggcaa tacagcatca actgctaatg cttcttcatt
    59641 atatcccgac tcaggaacat ttgcggttac acttttagta actaatggta atggatgttc
    59701 agacgactca gtaagaaacg tagtagtaaa attatcgcct acggcaagtg caaccacaaa
    59761 cgatacaaca ggttgtaatc catacacaag tacttttgta aataactcaa tcaacgcaac
    59821 ttcttataac tggaattttg gtgatggcgg aacatcaaca caagcaaatc caacacacac
    59881 ctatcccgga acaggaaact ttactgttac actgattgca accaatacag caggatgtaa
    59941 agacaccttt gtgttcaaca ataccattca ggtaaatcaa acaccattgg cttcatttac
    60001 tgcttcagct gtaaccggat gtgctccatt aaatgttaac ttcaccaata cctcaaccgt
    60061 tacttcggga gctacatact tctgggattt tggaaatggc aatacttcaa cagttgctaa
    60121 cccttccgct gtatttgctg attcaggtac ttatcaggta gtattgaatg taaacaatgg
    60181 caatggctgt actcatacag catcacaact aattaccgtt aatatttctc caacagcgct
    60241 tgcattaacc acagacactg caggatgtac accacatgct gtgagcttca ttaataattc
    60301 taccaatgct acaagctatc agtggaactt tggaaacgga gcaacttcaa cagcagtaag
    60361 tcccagctat acttatacta caggaggatt gatgacggtg acattaattg caagcaattc
    60421 tgcaggatgt cgtgatacac ttgttttgcc atatcagatt aacgtaaagc aatcacccag
    60481 agcaagattt aatatgagtc ctacccttgg ttgcacacca ttgaatgtga actttaccaa
    60541 tactaccgcc aatatgcaag gtgctacatt caactggaat tttggtaacg gaacttcagc
    60601 aacaactgca aatccttcaa cagtttattc agactccgga acctatgcgg ttacattaac
    60661 agttactaat agcaatggtt gctctgatga ttcaacccga actgttcgtg ccaatctttc
    60721 gccaacagca atagctgcta caacagatac tgcaggttgt gcaccataca caagtacatt
    60781 tacaaacagc tcactcaatg ctacttcata tcaatggaat tttggtgatg gtggtacatc
    60841 aacggttgca acaccaacac atacgtataa ctcatcaggc aattatactg taacattaat
    60901 tgcttcaaat gcatcaggtt gtagagatac attagtgttt ccgtatgcca tcaatgtaaa
    60961 taataaaccg gtagctgcat ttacacctgc atctgcatca ggatgtgttc catatactnn
    61021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    61081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ttttacaaac tcatcaacta
    61141 atgcaacatc ttatatatgg aattttggaa atggattaac atcaactact ccaaatccaa
    61201 cacacactta tacaacaggt ggattataca ctgtgacttt aattgccatt acttctaatg
    61261 gctgccgtga tacatttaca cttccatatc agataaatac aaagcaatct cctttagcaa
    61321 atttcagtca atcagctaca acaggttgtt ctccattggc agtgactttt acaaatactt
    61381 caaccaatat ttccggtact gtatggaact ggaacttcgg aaatggtgca acatcatcac
    61441 tacaaaaccc aactgcattc tatcctgaca gcggtaccta tcagataacg ctttgggcaa
    61501 tgaacagcaa tggttgtatg gatgatacgg tgagaactgt aagagtaaat caaacaccaa
    61561 cagcaatagc cgctacaaac gataccgttg gctgtacacc atacaatgta aactttagca
    61621 ataactcatt gaatgccgcg agctataact ggaattttgg tgatggaata acctcaacaa
    61681 ctcctgttcc taaccacaca tataccaatg caggaactta tactgttact ctcatagcca
    61741 catcaagtgc tggttgtaag gatacattga ttttacctaa caacattaca gtaaatcaaa
    61801 ctcctgttgc ttcatttaca aaatcagtat caacaggatg ttcaccatta actgtaaact
    61861 ttaacaacac ttcaacacag acaaatagtg catcttatca atggagtttt ggaaacggaa
    61921 ctacctctac tcttacaaat ccaactgtac aatacacaga ctccggaaat tatacggtaa
    61981 cacttgttac taccaatgcc agtggatgtt cagacagtac atcacaacaa atacatgttg
    62041 acttgtcgcc aaaagctatt gcaacaacaa cagatacttt aggatgtagt ccgcatactg
    62101 cagtatttac caatagttct atcaatgcaa cttcatatac ctggaacttt ggtgatggga
    62161 caacttctac aacatctaat ccaacacata cttatgcaaa tacaggaaat tatactgtaa
    62221 cattaatagt aagtaatgct gcaggttgca gagatacttt ggttctgcca caaacaatac
    62281 aggtaaacca aacaccgatg gcggcattta caccatctgc aacttcggga tgttcaccat
    62341 tgacaataaa cttcaacaat caatcttctg atactatcaa tgcaaacttt gcttggaatt
    62401 ttggtaatgg aactacctca aatgcattaa atccaacaaa cgtatttgct gactcaggag
    62461 catttgctgt aacacttatt ataaccaatg caaatggatg caaggatact gcaacacata
    62521 acattcaggt gatgcaaaca ccaacagcaa acgcattcac taatgataca acaggttgtt
    62581 caccatttac tgctaacttt acatcactgc caacaaacag cgattctgtg gtatggcatt
    62641 ttggcaatgg cacaacagct tcaggaaata atgtacaggc aacatatacg cagccgggaa
    62701 tttatcaacc ttatctgatt gcttataatt ctacaggatg tacagacact ttctatcttc
    62761 ctaaaactat cagagttaaa ctttcaccgg ttgcagattt tgcggcagac caaaatgcat
    62821 catgctcagg aacaaacttc acttttaaca atctatcctc agatactttg tcaccggttt
    62881 atcaatggac aattggaggc ttcagtacaa acaatccttc attctctatt ccattgctga
    62941 caccaggatt ctatacggta gcattgacag ttaccaatag caatggatgt actgattcaa
    63001 ttgtgaaagc aaattacatt caggtatttg atacattacc tccaccacaa gatcctatat
    63061 atagtgtgag tgtaatatct gatacgcaag tagaaattaa atgggccaat agtgctgcat
    63121 tagatttagg tgcttataag ttgtggcgat tggatccttc aagtggaaat tatgtgaatg
    63181 tatatacaga taataatcca agtaactcta caatgaatcc ggagtcaaaa tatactgaaa
    63241 atggattgaa tacattgaac aatacttata cctacaagct tcaaacactt gacagatgtt
    63301 cgtttgcact accattaaat gtgctgaaag ctcatactac aataaacgta agtgcaaata
    63361 agagcagcaa tggtatcaat gttacatgga caccatatca gggttgtccg gtatcgcaat
    63421 atgaaatcag cagaactgaa gtgagcaatg gaagtagtgc tgttattgcc actgttcctg
    63481 catcaacatt atattacttt gattcaacaa tgatatgtcc tgatgattac agctacagaa
    63541 taaaagctta tgatctttgt ggaacaactt acacatcata cagtgataca gcagcagcaa
    63601 aacctgatta caatttgcag gagcaacaag ccatcattgt acgttcaaca gtagtgaaca
    63661 acaactttgt actgacagaa tggtctgaac caagattaca tccagaaaga gtaatgcaat
    63721 acaacattta tcgtagtact gacacaacag gaaacttcta ttcgttgctg gcaacagtat
    63781 ctgcaaatgt tacaagctac gaagactttg cagtaaatgt gaatgaacaa aactattact
    63841 ataaaataga agtaatcagt gattgtaata tggctggtca attaagcagt aacagttctt
    63901 ccatattgct tcagtcgaaa tgggagtatg caaacagcag actgtggtgg actaaatatg
    63961 ataaatggga tacaggagtt gaacgctatg aaatagaaaa atataactgg ggcacaggcc
    64021 aatgggaaaa ggttaaaaca gttccgggca atgctacaga aacagaagtc gatgagtaat
    64081 taagttattc attgtgtatg tcccccccga aacccgactc gagaagtcgg gtttctttat
    64141 ttatgtcata cagataatgg tgattacaaa aaaaattcaa ccctattcaa taagaaacat
    64201 tgaatggctt aactagttta tctgaaaaaa aaaagttact tgagtgattt aatgccgtga
    64261 tgtgaggcag caattaactg tctaacagtt ttcattatag attgcatatc aaaatgacat
    64321 tctgcatata attcagattg ctcaccatgt tcaataaatt gatcggggat acccagtctg
    64381 acaatttctg cagcatagcc attatcaatc ataaattctg caacagcact tcccattcct
    64441 ccttgcagac aaccatcttc aactgttata atctttttaa aattagcaaa aacttcgtga
    64501 agcaacattt ggtcaagcgg tttcacaaaa cgtaaatcgt aatgtgcagg ataaatattt
    64561 tcttttgcaa gttcatcaca tgctgctact gcaaaattac ccggatgtcc gaaagttaaa
    64621 atagcaacct gttcaccacc tcttaattta cggcctgttc ctacaggaat tgctttgaaa
    64681 ggtgtacgcc attctgtcaa cacaccatta cctcttggat aacgaataca aaaaggtcct
    64741 aagttatcat gttgtgcagt gtacatcaga ttgcgcaatt cttcttcatt catcggagca
    64801 cttacaatca tattagggat gcatcgcata tatgcaatat caaatgcacc atgatgtgta
    64861 ggtccgtcag caccggcaag gcctcctctg tcgagacaga aaacaacatt taatttttga
    64921 agtgcaacat catgcaccac ctgatcgtag gcacgctgca taaatgatga gtaaatatta
    64981 caaaatggaa taagcccttg tgtggccata cctgcactaa atgtcacagc atgttgctct
    65041 gcaataccta catcaaatgc tctatcaggc attgctttca tcataatatt taatgaacac
    65101 cccgatggca ttgcaggtgt tacaccaaca atttttgaat tcttttctgc aagttccacc
    65161 atggtgtgac caaatacatc ctgatatttt ggtggttgag gtgtgttgtt ttgcggctta
    65221 aaaatttcac cggttatttt atcaaacaat cccggtgcat gccattttgt ttgatcttgt
    65281 tcggcaagtt taaatccttt tccttttact gtaagacaat gtaaaatttt ggggccggga
    65341 atatttttta aatcttcaag tactttggtg agatggttta catcatgtcc atcaataggc
    65401 ccgaaataac gaaagcgaag tgattcgaat aagttgcttt gtttaagtac agccgatttc
    65461 attgcattct ctattttttg tgcaatagct tgtgcattgg gaccaaactt actgatgaca
    65521 cctaatgctt tccagatttc gtctttaact ttattatatg ttggtgatgt tgtaatgtct
    65581 gtaaggtatt ccttcagtgc accgacattg gggtcaatac tcatgcagtt gtcgttaagc
    65641 acaacaagaa ggttactgtt ctctacacct ccgtgattga gtgcttcaaa tgccatgcct
    65701 gctgtcatgg ctccatcacc aattacagca atgtgctgac ggcttttatc atcctttaat
    65761 ctgcttgcaa cactcattcc aagtgcagca gaaattgaag ttgaactatg acctacacca
    65821 aaagtatcgt aaacactttc gctgcgtttt ggaaaaccac taatgccttt atatattctg
    65881 ttggagtgaa aaacatcgcg cctgccggtt aatattttat gaccataggc ctgatgaccc
    65941 acatcccaaa ccaactgatc gttgggtgta ttaaaaacat agtgaagtgc aactgttaat
    66001 tctacaacac ccaggcttgc accaaagtgt cctccattaa cagaaactat atcaataata
    66061 aactgtcgta actccttaca aacctgttca agctgctccg gtttaatttt ccttaaatcg
    66121 tcaggtgtgt tgatactgct aagaagctct cctgctttaa tttccataca gcaaatagtg
    66181 atgcaaactt acaaataatg tatggaatag ccccaaatta ccaatgaaaa caacatatta
    66241 tttggcaaaa ggttgaaaat gtcactctaa agaaggtttt tcattcccgg attcttcagg
    66301 ttaatcaaac caatgtataa tttcattcag ggtaagtttc tcttttgatg tgctatctaa
    66361 atctcttatt atttgaccag atgccatttg aataattctg tttccgtagt caagaacctg
    66421 tttggtttga tgagtaacca ttaatatagc aaccggcatt tgatttaata tccgtgaggt
    66481 tagtttcata atgagttctg cacttttagg gtcaagagct gcagtgggtt catccatcaa
    66541 cagaagttta aaatcatcca ttgttgacat cactactgtc agtgcctgac gttgtcctcc
    66601 ggacagtgct cccatctttc ggtttagctg atcttcgaga ccaagtccta accatgaaac
    66661 tctttctctg atggagtcaa caaatttttt atcaatgcct attcttaaaa tctttgaatg
    66721 acttcgtaaa gccgctaaac ggaagttttc tataatagta aggtctgagg cagtccctga
    66781 ttgcggattt tgaaatactc tggcaataaa acgacttcgt tcatgttctt taagtgcagt
    66841 aatatcgttg ctatcaacag caattttacc ttcatcacac aatatagttc ctgcaataat
    66901 attcagtaaa gtagattttc cggaaccatt cgcaccaaca ataatcacaa attctttttc
    66961 ttttatacgt aacgatgtag gatacaaagc aatgacaggc ttttcgccat caaaaaactt
    67021 tttacttata tgagatattt caatcattgt gcaggtctaa aaataaattt aaaaactcct
    67081 gctgccagca acacaagaat tgctgtaagt aatttaagca tttgtggtgg cactccggca
    67141 agcaatgcaa atgctaaaag taatcgaaat aaaacagaac ctaataagac ggaaattaat
    67201 gcactcagta tagaagatga tttgaaaaat gctgtaaacg attcacccaa aataacagca
    67261 gctaaacctg ctataacaat tccaataccc atattaatgt cagcaaatcc ctgatactgc
    67321 accatcatat aaccgcttaa tgctgtcaat gcattggcaa ttgccaatcc ggcaactttc
    67381 ataaaattag tattaatgcc catagcttta accattaatt cattgctgcc tgttgcacgc
    67441 attgctatac cgaaatcggt tttcattagc cagaataaca acaacaacaa aacaatggaa
    67501 aacattgctg ccaataacat attaaaactt gtgttatcat taaagatatt tgcctcgttg
    67561 attaatggaa tattgggtct tcccataata aacaggttga tagaataaag tgatgtcatc
    67621 accaaaatac ctgcaagcaa cggatgcaat tttagttgtg tgtgaataag acctgttgca
    67681 acacctgcca aggcacctgc aacaagtaca atgggcattg cgtaagaagg atgccaatga
    67741 tttgaaatta aaactgcggc aacaacacca cccaatgtat agctgccgtc agtagtaatg
    67801 tccggaatat taaaaatccg caaggtcaga taaatgccaa atgccatagc agcataacag
    67861 aaaccttgaa taattgcagg aataattact tccattaggg tacaattacc ataaaaccat
    67921 tgggtgcaac aaggttaaaa cgagctgctg ctttattatt gaatacatat tgcctgacct
    67981 taacaatctc gggttttaac attgtggcag gattggattt aagataattt gctgcttcca
    68041 ctcctgcctg aaaaccccat tgataaaaat cggccccgta ggatgcaata gcaccacgac
    68101 caaccaaacc tgcttcgctg gtaaaaaccg gaagttgtgc ttcattgcat gctttaacca
    68161 ctgtttcgaa cgatgcaaaa atgacattgt cggggagtgc aaaaaataca tcgggctttt
    68221 tggcaatgag tgattgtgtt accaattgtg tttctgaaga atttgtaacc ggcatggcaa
    68281 taattttcat tcctgctgct gtagctgctt tttgcaattc ttcaagagca tctcgcgatt
    68341 gtggttcgct ggaattgtaa atagtgccaa cagtcattgc atcagggaag aaacgtttta
    68401 tcaatgcaac agagctgtca atatatgcca gagtttcaaa aacaccaaag aggtttttag
    68461 gatagtttcc ctgagcatct gtgagtcctg caagatcggg acgtggtgcc accatcatac
    68521 atacagcgat attttttgtg tgctgcactg ctgcaattgt tgaaagtgtt gaattggctg
    68581 caatgagttt tacatttttt gaaataagat aatcgcatgc ctgattcaag acagcaattt
    68641 ctccctgtgc atttgaaaaa taaactttta gtgttttgtc tttttcagaa aagccattct
    68701 ttgccaatgc atctaaaaat cctgttcgtg cttttgctaa agttgcatct tctgcaatat
    68761 ctaaaaatcc tattgacgga atattttttg aagtgttatc acatgatgaa acaaagcaac
    68821 aaattgcaac aatgattaaa tgcaggtttt tcataatgaa caatgaaggt ataaaactaa
    68881 gaaaatctaa actatcagta atgaaaaacg ggacgctgct tgcgcaaacg tcccgaagcc
    68941 atgaaaacta tcgttgttta tttattctct ttcttctctt catccttttc aacaccggat
    69001 gatgcgtctt taaattcttt tattccttta ccaagtcctt ttgctaattc aggtagtttt
    69061 tttccaccga aaaacaacaa caacaccaaa actatcagta gaatttcgga tccacctaaa
    69121 ctccaaagca gtaaacaagt aacagaactc attctttaaa tatatggtac aaatatacaa
    69181 gaaattataa tgttagatgc aaataatcgt taaattaatt ttttgctatc cacaatgcag
    69241 gattcagctt actaaagcct ttccatatct gaagattcat atcagcatta cctgattcag
    69301 agtcagtcct tacacgacca atcttttgtt ttgttgtaac tttctcacct ttagtgacaa
    69361 acacttcgtc aagattggaa tatacagata aaaattcgcc atgtctgata attactgccg
    69421 agccatttgg taaatttact gtgcctgtaa cttctccatt aaaaatactt cgcactgagc
    69481 taccttttga agttcggata tctattccat cattcttagt agtcacattt tttaataatg
    69541 ggtgctgatg ttcgccaaac gtctcagata ttcttccttt ttcaacaggc catggtaaac
    69601 tgcctttatt attggcaaaa ctgttgctta gctgctgcgc ttctggtgtt agtgtaaata
    69661 cattttcatt agtaacattc tttttacccg ctttcactgc ttttttcttt gctgcctcaa
    69721 tttcttttcg cactaatgct gcaatggcct tgtctaactt taccttatcc ctgatttttg
    69781 cttcaagctt tttgcgtaat gatttttcac ttgcctgaag
//
LOCUS       DAOQNP010000004        67930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_108_length_67930_cov_8.208147, whole genome shotgun sequence.
ACCESSION   DAOQNP010000004 DAOQNP010000000
VERSION     DAOQNP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 67930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67930
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_67930_cov_8.208147"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..730
                     /locus_tag="PKY09_01045"
     CDS             11..730
                     /locus_tag="PKY09_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11465.1"
                     /translation="MEDIFYNILWIDDEHESLTSTKGRAKSNGINLIGFKSLNSGMSE
                     LERNLHFYDGILLDAKFYENDDAIAGSEDTDNVHRAKERLLQLNKKFEIFVYTGQAEA
                     FEDKTFNKAFKRVYKKGSSDERDRLFNDIKLAAATQEDTQIRHSYKRVFDVCTEKYIG
                     ELAGQDILNLLKVNDETNIDNHFNAIRKVVEDLFIAFNKFNLLPAEFVTPGVALNESS
                     KFLAGKGSDGSLFSEKGYQHL"
     gene            776..1195
                     /locus_tag="PKY09_01050"
     CDS             776..1195
                     /locus_tag="PKY09_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11466.1"
                     /translation="MSVTQAGSHRSEIDVHVRTIKTPYLLKSVFFQLLDVLVWFKMYV
                     DSNPKTENWLKVETAAEAAEPAAELIHGKVINIHPQNGFAFFKPDAIGDNIFIPPHLV
                     TNHSLTEGMPVQVEVEEYTDNRTNELKNRVKRIEMRP"
     gene            1333..4419
                     /locus_tag="PKY09_01055"
     CDS             1333..4419
                     /locus_tag="PKY09_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNB11467.1"
                     /translation="MTFNEDSRVKIPAIVHLTRLGYSYLPKSAIANLHTDTNIFKDQF
                     KAGLSKINDREFTDTEIDSFLSEINIQLDNSDLGKRFYKSLLGDFACKLIDLENFENN
                     LFHVVTELTYKNEEEEFRPDITLLFNGMPLSFIEVKKPNNREGILAERNRINARFKNP
                     KFSKFMNITQLLVFSNNQEYDEESITPIQGAFYATPDPEQVNFNCFREEETAIIHSVP
                     AEDSEIEKQILLDNNIVSILGNSEYNTAKDNNSPTNRVLTSLFSKQRLKTILKYGFAY
                     VNSSDKIGNRMEKHIMRYPQLFATLAIEKKLNQGIKKGIVWHTQGSGKTALAYYNVSY
                     LKDYFQKQNTVAKFYFIVDRLDLAQQAKEEFVARGLTVEMINSKEDFINNIKTAGATA
                     TNVGVQTITVVNIQKFSDYSIANISDYNLNVKRIYFLDEVHRSYNPEGTFYEKLVNSD
                     REAIHIGLSGTPLITGDFKSKEIFGDYFHKYYYNKSIADGYTLKLIREAIETKFRTDI
                     NKVYDEIVKQGEFTKRDVFSHKKFVEPLVDYIIADFKKSRVIHNDNSIGGMIVCDSSD
                     QAKMIFKFVQKYNDAKTKLRQLTTEPNTQYTIAAELKEEYLNAEDAPIIAALILHDVD
                     TKQIRKDNQTDFKQGKIDLLIVFNMLLTGFDAKRLKKLYLTRVVKEHNLLQTLTRVNR
                     PYKNFKYGYVVDFADIRKEFDKTNKEYFKELSAELGDEAKEYSNLFKSAEEIDREIAE
                     IKEKLFLYDFSNLEEFQKIISRITDKKEITDLKKCLENLKSLYNIIRIMGYTELLDKF
                     SFDKVNKLYNEVANRIDIINLKENLEDANDNTNLLNIALEKMQFTFRKIHTHELQIAD
                     KFRSELERARKELEGNFDKKDPKFISLFEELKRLFKKKNIEELDSAEMDTAIKDLKNI
                     YEQANALNNKDAQLAAKYENDTKFAKIHKRIRENNLPVLNTDTELHEVLLTIKHKTDK
                     TVINNQAILNNEDFFTEATKRTIIEVMEEKGIRNLELVKFINQTLVNEYFEQRKAA"
     gene            4422..5402
                     /locus_tag="PKY09_01060"
     CDS             4422..5402
                     /locus_tag="PKY09_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNB11468.1"
                     /translation="MSNIQKDKLFQSVREIVLEARKNVYRSSNTILLKMYWEIGRLIV
                     DDEQKGASRAEYGKSVLKNLARQLTLEFGKGYDERNLNNMRAFYNSFQIWNAVRTELS
                     WTHYRIISRLENAANRIAYMNEAIAGNWNTRTLQRNIDSHYLGRLLETPGATGSSTPE
                     HYIKDPYIFEFLGLQQDSTLSETVLETALINHIQSFLMELGKGFAFVARQQHIATDTS
                     DFFIDLVFYNYYLKCFVLIDLKTNKLSHEAIGQMDMYVRMYNDLKKAEDDNPTIGIIL
                     CTEKDETIVKYSVLAENEKLFASKYRLYLPKEEELKELIENDRTKFELEK"
     gene            5399..7045
                     /locus_tag="PKY09_01065"
     CDS             5399..7045
                     /locus_tag="PKY09_01065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNB11469.1"
                     /translation="MTKHLEHTQKTKELIDGLKTVCSNYGLANTGYEYKIITEVFLYK
                     FLNDKFLHEARNANKKLKDSKNIEADIQSMTDNDYEYMLEKIGERSAKLKKTHFISYL
                     FNKQNEKDFSITFDTTLTEIAATNIKIFSVSTIGGERIPLFEELSENIREKNERSPFC
                     KAIINKLVAFSFEPVFEEKYDFFSTIFEYLIKDYNKDSGQYAEYYTPPAVAKIMARIL
                     VPKETKNVLLYDPAAGSGSLLLSLAHQIGEDKCTIYSQDISQKSSEFLRLNLILNNLV
                     HSLHNVIKGNTLTNPFHVNDAKDDLKKFDYIVSNPPFKTDFSDDRETLASDIHKKRFF
                     AGVPSVPAKKKESMAIYQLFIQHIMYSLDKKGKAAVVVPTGFCTEKAAIGLGIRKKLV
                     DNNWLHAVVQMPSNIFATTGTNVSVLFFDKGKKNNTAILVDASKLGTKVKDGKNQKTL
                     LSAEDENKIIDTINNYETVENFSIVVENEKIKTRNYSFSAGQFFETTIEYSEITQEEF
                     ENKMEGFENNLDSLFEQSKNLEESIRKQLLKIEYAQADKI"
     gene            7065..8261
                     /locus_tag="PKY09_01070"
     CDS             7065..8261
                     /locus_tag="PKY09_01070"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016899490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNB11470.1"
                     /translation="MSSGISSKPEQAGHGSPFASFSTVFNNYFLPDELPDLMDVSEQE
                     QETYSIKNGDILLTRTSETIDELAMSCVALKDFPKATFSGFTKRIRPTQTDVTYPKYM
                     GFYLRSNFFRRTMTNNAVMTLRASLNEDIFSYLDLLLPDFDTQKKIGDFLYLLDDKIK
                     INNRINTELEAMAKTIYNYWFIQFDFPNEEGKPYKASGGEMEYNEKLKMEIPVGWTDG
                     KLSEVANITMGQSPDGDSYNEEGEGMVFFQGSTDFNFRFPLVRMFTTAPSRIAQEEDV
                     LLSVRAPVGTLNVANEKCCIGRGLAALNSKNEANSFVLYLMFYFKAMFDVQDRTGTTF
                     GSIDKDTLHDLKFAKPPVELQKQFQSIVGKYDKMILTRSRETQELITLRDFLLPLLMN
                     GQVKLV"
     gene            8395..8793
                     /locus_tag="PKY09_01075"
     CDS             8395..8793
                     /locus_tag="PKY09_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNB11471.1"
                     /translation="MINEQYKYSELTSKIIGCAMAVHKALGNGFQEVIYQRALEIEMR
                     LMGIEFSREFEMPIFYWDEQIGTRRVDFLVEGIVSVELKAITKLEDVHFAQAINYLEA
                     YNLEIGLLINFGETSLNFKRLTNKKFKPIK"
     gene            complement(9359..11044)
                     /locus_tag="PKY09_01080"
     CDS             complement(9359..11044)
                     /locus_tag="PKY09_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11472.1"
                     /translation="MQQQKSLIIFILFILCFLFASDIKAQQYKFAKRVGGTQFENAMG
                     IALDSMGNIIVSGYFSGTADFDPGTTNYTLTSNGGNDIFLAKYDSAGNFVWAIHVGGA
                     GEEFNYTDPAVDEYGNIYLCGVFNSNTDFNPKGNAVIATNRGQQDGFVAKYDSTGLLQ
                     WVRGVGGALNDDVYRIDYKNYLVLFAGSFADSSYVDDGLTITPLYGSGSADVVFGKFN
                     PNGNLFWLNTLKGNGEDHSYNITAGINGKTYLTGTFEDTLIFDSGGVNPDTLFADGLT
                     AFTASFSNFGNYYWSFPIENAYPFGLKVDHNGDILTCGQFSNFADFDPDADTMTLIAQ
                     GSFDGYFAKYTNGGNYVFAKRIGGNGSDICYTIAELSNSSILVSGYFFNTADFDPDVT
                     VASLNSNGFADMYLAHYDSLGNYLSAFGCGSPAFEFCRNMTCNGIDEVFLCGGFEQVV
                     DFNPAPAINTLVSAGSRDGYFVKYAFPTTSVHNMSSADLLIYPNPFVNQINISDNFKQ
                     TQLILTDVLGNIIYKAIISAQTKINTELLSPGIYFLTIASGENVSTRKLLKVK"
     gene            11128..12378
                     /locus_tag="PKY09_01085"
     CDS             11128..12378
                     /locus_tag="PKY09_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB11473.1"
                     /translation="MEHSTKTFALKNGLQLVLTSLSNTEVSHCALLIKAGTRNEQIQQ
                     EGLAHFIEHMLFKGTRKRKSFHILNRLEVVGGELNAFTSKEETCIHASVTTPHLERAL
                     ELISDITFNSVFNSLEIEKEKEVVCDEIRSYLDNPGEQIFDDFEGIVFKKNPLGNPIL
                     GTENSVRNFKQKDLIAFTRKFYIPSNMVLSVASSLKAEKVIRQLEHHFGKYRLAKVGE
                     KPKPFKKYTAEKTAVKKHNAQCHFIMGTLAPSLYQADRMTVSLLNNILGGPGMNSKLN
                     LGVREKYGYTYAIESGFNSYTDTGMFHVYLATDKKFLNKSIDLAVAEMKKLTDNKIGT
                     LQLSQYKQQLKGQLALAKENKSNVVISNARSLLNFKKVTTLEDIYKKIDAVTSSQLVE
                     AAAKYLNAKSRSYSSLLYESVSHE"
     gene            12323..15124
                     /locus_tag="PKY09_01090"
     CDS             12323..15124
                     /locus_tag="PKY09_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNB11474.1"
                     /translation="MQNRAATVACFMKALVMSNNTASFLTRIVDELIATVDGNNFADH
                     TVIFPTRRAALLFKLQLAKKLNKAILLPSVLSISDFVEYYIPLRVSEKKEILLDLYAV
                     YKPHFPDYDFTSFAPWGEMILSDFDEIDLYLVNYKDLFADLTSYHEIDSVFQGDEEFK
                     EQLKPFFELFMADKTSTLKQNFLYAWHALPKLYKELNEVLEKNNKTTAGKAMKQLALS
                     PEIFLKNLDCSKTIIAGFYALAPAEQKLFEFILNGKGKIFWDADAYYTNNTTQEAGLF
                     FRKNVLTQNSFKWKETYFKDVPKNINVTGVPLITGQFKFLADRLKTLIAQKKFNPDKT
                     AIVLPDEKLLPLMLQSLPEDIEHVNITMGFPLKETHLYRFIETLNALLKQIPNTSKQK
                     LVPTSALGNYLAHPYASLLKHNMCNINDAGLSIPLIELPENISQFLKLQITTPVFERY
                     IQLLTMLHDAMESKHHEKEIAGFVLNELLELRTMVAPYAEILDEPSLQMLISEMLDTT
                     RIPFSGEPVMGLQIMGLLETRTLDFDNIFFLNVNEGSVPKSNRHHSFIPFTIRKSFGL
                     PLVEEQDAITAYHFWRLMQRASEIDLIYNTEVNEFASGERSRYLLQLFYEMKAQFSNW
                     QVNHQIITTPLHRYNYISPGIKRDEHLTKHLLSMVAENKLRFSASSLNNFIYCSLKYY
                     FNEIKKIRNPDDTEEEIDNKVFGKILHDAVSKIYAQIIDVEFDKNIAAQLRKSISTFV
                     KASIAQEYDKHYHGTGYTVIIEKIITEYIERIIASDLKGVTFSPHEIETEKVVVCDIG
                     NGINVKIKGVFDRIDKVYDGYRIVDYKTGGDEVKDVKKPDDLITNPNYKVNFQLFLYC
                     WLFLKSHDKEKVNAGIYPLRRMEDGLKMLKQEQIADHSMFEELLFTLIRKILLESDFT
                     MTTDTTRCKHCPYKDICNR"
     gene            complement(15125..15526)
                     /locus_tag="PKY09_01095"
     CDS             complement(15125..15526)
                     /locus_tag="PKY09_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNB11475.1"
                     /translation="MKPFVVRVYGILINKNSEVLLTHEKEGGIEFTKFPGGGLEFGEG
                     LRECLVREFLEETQQKVEVKEHFYTADFFIESAFNPGLQLISVYYKVLCENFILPEDA
                     NNIHFFWKPLKELNGEVVTFPVDRIVEKLLM"
     gene            complement(15595..16461)
                     /locus_tag="PKY09_01100"
     CDS             complement(15595..16461)
                     /locus_tag="PKY09_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11476.1"
                     /translation="MKNFLKLSVVFLLMAMYACKDYKPEVDRLNRERDSLISTSFLKD
                     STIDSFLGDFNGIEASLDSINQIHAAITLDRKRDPEMGGTVKDRIRKNMESIASLLDQ
                     NSQRIAELTRKLKNSGVKSAQLNKMITSLNLKIAERDSMIANLNNQLGDAKIEITGLR
                     TNIETLNSTIGERDKTIEEKVNQINTAYYAVGTYKQLRDKKILNKKGGFIGIGKTKTI
                     SPDIDNSAFTKVDIRQFASVELNTKSVKIMSSHPSSAYTYEKDKKKYQSLKITNPDLF
                     WSNSKYLVIVTK"
     gene            complement(16587..17909)
                     /locus_tag="PKY09_01105"
     CDS             complement(16587..17909)
                     /locus_tag="PKY09_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNB11477.1"
                     /translation="MRKLFLALVLLFFMVTNVCAGIVYRFNFQSEVDRGMARIFSKAL
                     REAHEQKADLFLIHLNTYGGMLDAADSIRIAILNSKIPVVVFIDPNAASAGALISIAC
                     NRIYMRSGSSIGAATVVTEQGEAAPDKYQSYMRGIMRATAEKRNRDPRIAEAMVDPRV
                     VIPGVNDSGRVLTFTAEEAMTNKYCNAIVETEADILKLENLNNDKIIEFQPSWVDNMI
                     SFLIHPALSSLLILIMLAGLYFEFQAPGTIFPIAASFVAAILYFAPLYLEGVAANWEI
                     LLFVSGVILLALEFFVIPGFGVAGIAGIICTTMGFALSMINNKGWDFTFTSSEQITQS
                     ILISLGAMFGAVLLIFYFIGKINHSKRLSKLTLQTTQPSEHGFRAHTDIDAIQPGMRG
                     TAYTALRPSGKIMVDGRVFDAVAETGWIERQEQIEIVSVGLTCTVRTC"
     gene            18055..19047
                     /locus_tag="PKY09_01110"
     CDS             18055..19047
                     /locus_tag="PKY09_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB11478.1"
                     /translation="MKIKVSLLLATAALSLSAAVAQEGFRTIAGKKCQLYKVVSKDTW
                     SGLSRKYHKEIDELKAVNPGINDLKIGQIINIPIENTVNDKSENKSDTSADIKTRKPV
                     ATINTPAKSNNKIHVIEKGETLFGIARKYDVSANDIRKWNHLENDGIKAGQKLIVSTN
                     ENITVKTEIKKQETTNETILQPLDDKKLEAKNSEVKNDSKLIAEASNKLIPPSPSPSE
                     LEYKPLRTEVKRSGGKTGAVIEVNETGMASWIRDGDINQSKFYALHRNAPPGTIIKVT
                     NRMNGDYVFVKVVGTLPDTGDNDKQILKMSEAAAKRIGAINEKFQVELNYGTTN"
     gene            19198..20376
                     /locus_tag="PKY09_01115"
     CDS             19198..20376
                     /locus_tag="PKY09_01115"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNB11479.1"
                     /translation="MKEVYIVSAVRTPMGSFGGKLSSFSATRLGAMAIKGAIDRINLN
                     PSEIKEVYMGSVLQANLGQAPARQAAKFAGLPDTVQCTTINKVCASGMKAVMLAAQSI
                     MLGDNDVIVAGGMESMSNVPFYMQNMRWGNKYGDVSAIDGLAKDGLTDVYHNYAMGNA
                     ADLCARECNFSRQDQDSYAIQSYKRSADAWAAGKFKDEIVPVEIPQRKGEPILFAEDE
                     EYKNVHFDKIPGLKPAFSKEGTVTAANASTMNDGAAALILMSKEKAESLGLKPLGRIR
                     SYADAEQAPEWFTTTPSKALPRAVEKAGLKMTDLEFVELNEAFSVVALANMKEMKLDP
                     AKVNVNGGAVALGHPLGCSGARILVTLLNVLKQNNAKIGGAAICNGGGGASAMVIERM
                     "
     gene            complement(20487..22073)
                     /locus_tag="PKY09_01120"
     CDS             complement(20487..22073)
                     /locus_tag="PKY09_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11480.1"
                     /translation="MKSLTTFIITCLIVTLQVSAQNNCSNPLQVNICPSVTLYNQTNA
                     GMGDDLTGPSCNIVGEDVLYQVNIANGASKLIVSIQNCTGPFRVFAEKVCGSCMPLGS
                     IYTTQPMCNFSVNTANDTVLYVWVDAAATITYDIAFGSDTNFTVINIPNTQGNLSFDG
                     SVCATPPFEPTKPFFQIAVNGVYDTAPLFFSPLNVVDTVCITTFFKNTTGVEGVKRFV
                     FDFPASGYLNPQTAPIIPGFYNAGNWVATVSNGGQTWTLQFIDAAGIGRGDFTGTPNS
                     CLAYEFCFTLTPLSNLPQYTNATVNITSDGFGAGFSGWIQQGCCPSFFTNCSTNMTGG
                     GAVGGAHGFGFGFNDPGALPVELLSFTAKAEKNKVKLNWVTASEKNNSHFTIEKSVNG
                     NNWSKVAEVNGQGNSNVIHDYSYTDSYPISGASWYRLSQTDYDGTSETFPAQRVYVKP
                     FHYSIFPNPVKDLLTIQFDTDETPRIQMFNPLGNEVFVAVENDVGLVRLNTSEIPVGL
                     YFVSITIDGVQSKEQIAIIR"
     gene            22408..23124
                     /locus_tag="PKY09_01125"
     CDS             22408..23124
                     /locus_tag="PKY09_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNB11481.1"
                     /translation="MNKNIIQDIRIELKNISSEEVRIIASRFFKPGEMAKVYGVKSPI
                     VRTIGKKYLQQVKILTKKEIFAICEELWNSFYLEESVIACMFTESIHKKFEAGDIKTF
                     ESWIKNYVNNWASCDTFCNHTVGDYMMMYPEKTELLKKWAVSSNRWLRRAAAVSLIVP
                     AKKGLFFPLIIDIATILLADDDDLVQKGYGWMLKSACQHNEKDVFDFVMLHKSEMPRT
                     ALRYAIEKMPERLKSQAMKK"
     gene            23225..23923
                     /locus_tag="PKY09_01130"
     CDS             23225..23923
                     /locus_tag="PKY09_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNB11482.1"
                     /translation="MKFTLRLFLLLCFTVTRLAAQDENINTDRPDQSDGVYTLTKKYF
                     QVEEGITLGKNLFTNNLMIRYGLTTSTEIRLLSDAGKIDDWKGFLPFTLSVKQKIIKQ
                     KKWIPAITAVGYMSYGKIASKDFLTNKISYELKLAFENEVNENFTVSYNAGFTDDFKT
                     FNYTFNLGYSPIEKISLFAEYFASVNSSVQHNADAGVLVLLHPKLQVDIACGHSIADS
                     DNRFYTTFGISYKF"
     gene            23931..24365
                     /locus_tag="PKY09_01135"
     CDS             23931..24365
                     /locus_tag="PKY09_01135"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNB11483.1"
                     /translation="MKHFKSFIFILLTFCTSNLLAQDKYIPVSQTPEQIKTYIKNHFP
                     ATTISSVKKEKELLNYELEVKLNDSTKLTFNKDNALIEIESKKAIPEKILTDTIYTIV
                     KENYPNNKIVGWEKTKLGQKVELDNDVELYFDDNGNFIRTEH"
     gene            24528..27377
                     /gene="carB"
                     /locus_tag="PKY09_01140"
     CDS             24528..27377
                     /gene="carB"
                     /locus_tag="PKY09_01140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNB11484.1"
                     /translation="MPKDNSIKSVLIIGSGPIIIGQACEFDYSGSQASRSLREEGIEV
                     TLINSNPATIMTDQVTADHIYLLPLTVDSIEKILNERKIDAVLPTMGGQTALNLCKEA
                     DERGIWSKYGVRIIGVNVAAIETTENRESFRKLMIEIGVGVPPSAVANSFLEGKEIAQ
                     RIGFPLVIRPSYTLGGTGGSFVMKKEEFEDRLMRGLQASPIHEVLVEKAVLGWKEYEL
                     ELLRDANDNVIIICSIENLDPMGVHTGDSITIAPAMTLSDHTYQRMRDMAIKMMRAIG
                     KFAGGCNVQFAVSPDTEDIIAVEINPRVSRSSALASKATGYPIAKIAAKLAIGYHLDE
                     LKNQITGSTSAFFEPALDYVIVKIPRWNFDKFHGADRTLGLQMKSVGEVMGIGRSFNE
                     ALQKACQSLEINRHGLGADGTGWHKVDDIIYSLEHPSWDRIFHVKDALKLGLPIQTIH
                     NHTKIDKWFLTEIQKLVGMEQELRRHSLKNLSTDFMLELKQNGFSDYQIAHILGNVTA
                     EEVYETRKEMGVKRIYKMVDTCSAEFAAKTPYFYSTFERPAQDSNGNEITASESIVSN
                     KKKILVLGSGPNRIGQGIEFDYSCVHGVLASKESGYEAIMVNCNPETVSTDFDIADKL
                     YFEPVYQEHIFELIEYEKPEGVIVQLGGQTALKLSQYLNEKGIKIIGTSYEDMDLAED
                     RGRFSDLLHDLGIPYPPYGVAESAEEALVVANKVGYPVLVRPSYVLGGQGMSIVINDE
                     ELERAVIKLLKNLPGNRVLIDHFLDRAEECEADLICDGKDVQIIGIMEHIEPAGIHSG
                     DSYAVLPPFSLSDVVISQIKDYANKLALALNVKGLLNIQYAVKNEKVYVIEANPRASR
                     TVPFIAKAHQVPYINIATKVMLGVKKLKDFNIKPAQFGYAIKEPVFSFEKFADVNKEL
                     GPEMKSTGEAIRFIESTNDAYFRNLYKEKSMYLSK"
     gene            27396..27596
                     /locus_tag="PKY09_01145"
     CDS             27396..27596
                     /locus_tag="PKY09_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11485.1"
                     /translation="MRDVFYTIVVVWVVIQIYKSFVRPKSNQTQRNTTRQEGDVTINT
                     NTNNNNRNNDQGEYVDFEEIKD"
     gene            complement(27599..28759)
                     /locus_tag="PKY09_01150"
     CDS             complement(27599..28759)
                     /locus_tag="PKY09_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB11486.1"
                     /translation="MSGTVLAIAVTSIALSVMVMLVAVAIVTGFKKEITSKVVGFGAH
                     IRIKHFDNNQSYEEIPVNIDQKFLAKLKSDTRISSVNAYATKAGIIRTSEDILGVAVK
                     GVDSTFNSAFFDPNIIEGSFNLHIHSGDYPAIISKSIADKMKLKLNDYFVVYFIDEPP
                     RARKFKVNGIYSTGLEEFDNLYVFCPLTVIQKLNNWNVLQAGGFEVNLKNISDLESVN
                     ESIYKEAGFELNTQSIKTIYPEIFHWLELQDVNVIIILVLMILVAGINMVSTLLIILL
                     DNTTTIGLLKAIGAGNSGIRKIFLMVSSGIVGIGLIAGNVAGLLLCWMQYRFHLVSLP
                     QESYYVPYVPVNLSVTNILLINAGTLVVCILVMLIPSMIVSRISPVKALRYE"
     gene            28922..30040
                     /locus_tag="PKY09_01155"
     CDS             28922..30040
                     /locus_tag="PKY09_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNB11487.1"
                     /translation="MIDSVITGAGQVNSYLPIISDKNIAVVANQTSIVGKTHLVDLLL
                     SKHVKIKKIFTPEHGFRGTAEAGENIESGKDKSTGLPIISLYGNHKKPTTDDLKGIDI
                     VLFDLQDVGTRFYTYSSTLQYVMEACAENKKKLIVLDRPNPNGYYVDGPVLEPEFKSF
                     VGLNPIPVVHGLTMGEYAQMLNGEGWLANKEQCDVTVISVKGYTHETRYKLPVNPSPN
                     LPNMTAVYLYPSLCFFEGTIVSIGRGTAYPFQVVGYPGFTEGPFEFTPKKNPEMKLPP
                     LYENQKCKGFDLRDFGDYYIPSLKKLYLFWIKNIYDNYQQKDKFFNDYFDKLAGTDKL
                     RKQIIAGLNEEQIRRTWEEGLKSYKVIRKKYLLYPDFE"
     gene            30143..31069
                     /locus_tag="PKY09_01160"
     CDS             30143..31069
                     /locus_tag="PKY09_01160"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNB11488.1"
                     /translation="MKIYNHIDQFEKVSNAVITLGTFDGVHLGHKVIIERLKKIAGDV
                     NGQVVVLTFFPHPRMVLFPDDHGIQLLNTINERTMLMEKAGIDHLIIHPFSKEFSRMS
                     STEFVRDILVNKLGVKKLIIGYDHHFGRNREGSFTDLEELAPVYNFTVERIHEQLVND
                     VAVSSTKIRNSLLAGDVATAREFLGYDYELTGTVVHGKKLGHSLGFPTANLHIHEDYK
                     LVPANGVYAARTLVHGQWYDGVMSIGNRPTFDNGARSIEVNLFDFNADIYGEKITICM
                     TEFIRSEQKFENSAMLIEAMNADKLAAIKLLS"
     gene            31089..31691
                     /locus_tag="PKY09_01165"
     CDS             31089..31691
                     /locus_tag="PKY09_01165"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNB11489.1"
                     /translation="MKRNIIILLILLFASPAHAQELWDDAEIDTLPVFRSLNAAMQNP
                     DSVFRLDLSKQKLKEIPPQVFELTQLRELNVSRNHLTELPEQIYKLNNLQRLYANNNK
                     LTALPQRIGTLKNLQTLTLDRNRIEVLPPEMGMLHNLEVLSLWDNELSEIPDEIKSLS
                     SVLKKLELRGILFNEEQQKRIHDLLPYTTIYFSPACNCKQ"
     gene            31725..32243
                     /locus_tag="PKY09_01170"
     CDS             31725..32243
                     /locus_tag="PKY09_01170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB11490.1"
                     /translation="MLQTDKLHLRACEPDDLPLMYQWENNADYWLLSDTTKPFSNYTI
                     AEFIKQDQNDIFTTKQQRFVIELRNPVKPIGFIDLYQFEPVHRRAGVGILIADLSEQK
                     KGYAAQSLECLKNYSQTVLNMHQLYCYIQASNKKSLSLFQKAGFHKCGILKDWLINNK
                     TPEDVLMFQIIF"
     gene            32328..32723
                     /locus_tag="PKY09_01175"
     CDS             32328..32723
                     /locus_tag="PKY09_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNB11491.1"
                     /translation="MQTLSATIDKVKEFHATFGLQYFENPQAAIDKNIISLRHKLMQE
                     ENDEYLEAAMNNDLEQIADALGDKLYILCGTILAHGLQHKITEVFNEIHRSNMSKLDE
                     NGKPIYREDGKIMKGKNYFLPDIKTVLEQ"
     gene            32720..33430
                     /locus_tag="PKY09_01180"
     CDS             32720..33430
                     /locus_tag="PKY09_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB11492.1"
                     /translation="MIQKLSIIIPVFNESDTITTVLEKVNTVQLINNISKQIIVVDDH
                     SSDNSLEAITQFKNKHPHTEINIVQQQVNMGKGYAVRMGINVSTGDYIIIQDADLELN
                     PEDFNLLLKTSIERNVQVVYGSRFLRKQKFKVPFMTLMANRILSLVTSLLSGKKITDM
                     ETCYKLIERSLLHNIQLKENRFGFEPEITIKLLKNKRVRFAEVPISYQPRTKKMGKKI
                     GVKDGIRALYCLFRYRIL"
     gene            33427..35202
                     /locus_tag="PKY09_01185"
     CDS             33427..35202
                     /locus_tag="PKY09_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11493.1"
                     /translation="MKRSIIFIALFAFLTLIAFVWQQRTFGNEKYERIVDSDGKGYVA
                     YLPAIFIYQDLSFNFYSKPQNQKIARYFNDRFLTKQNDKTILKTYVGVAVLMTPFYLT
                     GCLMQIVIHGTCNGYEPLVQFFISIDAIFYALAGLFLIYKTLRLLNFSKTICVLTSAL
                     IFTATNLCYYSLFHPTMSHVYSFFLIAAFVYLSICLTQNTSNKYLLLSAIILGLIVLV
                     RPLNIIIILFPLLLLGKQKLTDLLKIKTRTLLVSVLFFLMIISLQPILNFLQCGLLFP
                     WSYGDEGFYFTNPTLIKTLFSFRNGLFIYSPILLLTIPGLWLLRYNSIKITSVIAIVL
                     IIIWYLLSAWWNWSSEPAFGNRSFVDWYGLMSLPVAASIGYGWKKSKLLLSLLCLGFA
                     GLSTLQTWQYLNDIIHPDGMNFEKYRYVFLKTSPEYIASVGNGNEEAYLPVQPLPFIT
                     TEINPYNKVTTSNHAETIFEFNSQTYKGAQFSIPFSNNQLLPMKAMVTLQRKENEFKA
                     CTQATVCFDYTDSTGKVYYNENLRINDIPRKQINAWKAFTYHFVMPALKDTNSQLNFY
                     INNPKGALFEIKNIDLKLFNFSNKQ"
     gene            35245..36681
                     /locus_tag="PKY09_01190"
     CDS             35245..36681
                     /locus_tag="PKY09_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB11494.1"
                     /translation="MQRKFITNLALLLLLNLLIKPFWIFGIDRTVQNMIGAEGYGKYY
                     ALLNFSFLLNIILDAGITNYNNRNISQHQHLVSKHFSGIIILRFLLAGAYILISLILG
                     LTIGYSKEDIHLLLILLANQFFISFILYLRSNLSGLHLFKQDSVISVTDRFLMILICG
                     YLIWYNNGANLNIGNFVYAQSASYLITAIVAFILLQGRISFLKLNWQPAFYIAILKKS
                     LPFALLILLMTFYSRIDSIMLERLLPEGAFYAGVYAQAYRILDAVNMFGFLFASLLLP
                     MFSHMIKKRQDIQPLTNLAFKLLMIPALTLAALGLFFSNDIMNLLYHEHGDLSAPVFS
                     ILMFSFVFISTSYIFGTLLTANGSLRNLNLTAFGGMILNISLNLYLIPQFKASGAALA
                     GLITQIIMAVAQIILAAKILHWKPDWKLNSKLLLFFILIALIADIAIRYFPEIKTTLV
                     IILLAPILAFSLGLLRISELKSIMKERQ"
     gene            36732..37703
                     /locus_tag="PKY09_01195"
     CDS             36732..37703
                     /locus_tag="PKY09_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11495.1"
                     /translation="MNNTPVYEENFASLSLIQLIKKWKKQLIIVAVISIVLSFIFTLP
                     FFIPPMFKSTAIVYPVNLSPYSTESATEQLLQLLESDKIKMELIKDFKLYAHYKIDST
                     KKTSLTTVFKQLAGNLSINKTDFESVKIEVMDTDPFQAKQMADSIIEKGNRLARNLYR
                     EKTMEGYIVAKTQYDMKKAELDSADKVLTELRLKSGIIEFSQQTREISRAYYTSILNK
                     SNNVRLEDMFHKLQQYGSDYVTLNEKINYTRSVFNNYKQYVENAEKDLTKELSYSNVV
                     TPPMIPDKKAYPLRSLIMLMFTASVVFLSFVVIIIYENSQEKKTQSN"
     gene            37708..39159
                     /locus_tag="PKY09_01200"
     CDS             37708..39159
                     /locus_tag="PKY09_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB11496.1"
                     /translation="MQVIQHKSLRWFYGLTAIYMLMAIFSLVNEFYWLLILPAGILIG
                     YSALYHLDKLFLFTAFCTPLAINLSKTDLGIGVSLPTEPILFGMMLIYLLSKSIDGRF
                     EKSFIKHPITILILIHLAWMLITSFTSSMFLVSIKHFVARLWFVVIGFMLATSVFKDE
                     KKMRQFIWFYAVGMAITIIYTITRHSINGFTQQSAHWVMTPFYNDHTEYAAVLALMLP
                     PIFGLMLLKHKSFMYWFFAFLISGLVIVAIVLSYTRATWLSLAVALVSMFAYVFRVKT
                     WAVVAIIISMVVGFQIFQSTVLYKFGKTKEYSQNDYRGHLKSITNIKTDASNVERLNR
                     WSCAWRMFLDKPIFGWGPGTYQFQYSVYQLQFEKTYISTNLGDKGNAHSEYLGPLSEQ
                     GFMGTIIFVALAVVVLYKASRVFLQSNSNNIKVLALSMQIGLITYLVHGFLNNFLDKD
                     KASIPFWAFVAAITAMDYYHNKKQLNPSDERAQ"
     gene            complement(39139..40365)
                     /locus_tag="PKY09_01205"
     CDS             complement(39139..40365)
                     /locus_tag="PKY09_01205"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB11497.1"
                     /translation="MEETTDKLASELQSKKVQLHWLLQITKAINYNLPAEQLYQIYEN
                     VLRDHLKAGKLSLFVNENGWKQVLSYGMKAELGRLQPELFLNEISFSKTKTVATPQWL
                     EKDESVIPVYHNEMLLAYCIVGAIDPSQSISIKEVVPFIHTITNIIVVAIENKRLNKE
                     AVAQAGFRKELELAAEMQEMLFPVGLCGNEYFEIATHYLPHQQVGGDYYDFISLNKHE
                     HIFCMADVSGKGMAAALLMSNFQANLHALIKNRPVLTNLVAELNDCVSKTAKGEKFIT
                     FFIGLINFKKGEINYINAGHNPPVLCSKNKSELLDKGTTGLGMFDELPFINEGHVKIS
                     SGDSVFCYTDGITELENLKGDFYGMEALKLFIEQNINNSSLDKFHERLIADLDQFREG
                     NSFSDDVTILSLRSLI"
     gene            complement(40368..41306)
                     /locus_tag="PKY09_01210"
     CDS             complement(40368..41306)
                     /locus_tag="PKY09_01210"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNB11498.1"
                     /translation="MKIVPAIRLADVASLLNAKYIGDDGFQITGINEIHKVEKGDITF
                     VDHPKYYDKALNSAATTILINKEVSCPEGKALIISDDPFRDYNLLTRKYRPLTFSLQQ
                     VDQTAVVGEGTVLMQGSVVGAHCKVGRNCLIYPGVVIYDHCEIGHGVIIHANSVIGAD
                     AFYFKRRPEGFDKMHSCGRVIIGDSVEIGASCTIDKGVSGDTVIGSGTRMDNHVHVGH
                     DTVIGKNCLFAAQVGIAGVVTIEDDVILWGQVGVQKDLTIGKGAVVLGQSGVGKSLEA
                     GKTYFGSPTTDARLKMRELALMKRLPEVFEFLDNKK"
     gene            41503..43863
                     /locus_tag="PKY09_01215"
     CDS             41503..43863
                     /locus_tag="PKY09_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB11499.1"
                     /translation="MKKFFVLTYTIFCFTFSFAQKPTQTVRGTITDKQSKEPLIGASI
                     VWLDTSIFIGTTADINGNFRLNNIPVGRQAFKISFLGYRETVITSIITSGKEVVLNIE
                     LEQSVVTAKAVTIVAEKQKDKPNNELTTVSARSFTIEETSRYAGSVGDPSRMAANYAG
                     VSGANDSRNDIIIRGNSPTGLLWRLNGIEIPNPNHFGSLGTTGGPISILNNNVLDKSD
                     FLTGAFPAEYGNVLAGVFDLQMRTGNNEKNEFMAQVGFNGFEAGAEGPISKKQGSSYL
                     INYRYSTLGVFSALGINFGTGTAVPKYQDVSFNINLPTNKAGKFTLFGMGGISGIEVL
                     EKDRDTTKKNLYVDDKRQNGYFDNTMGVVGLMHTFFINNSTYTKFSLSASIAGQKYKV
                     DTLPDFVDYAVPYYRNKSDQQKYSADFSLNKKFSSKNFLKTGFSFSQYHYTYRDSGVV
                     DSVWESYTDYNGNALLTKLYVEWQHRFSDQLSLNSGVYAQLFNYNNTYSIEPRIGAKW
                     EISDKQSLSFGTGLHSQLQPMYIYFVKTQKDDGSYYETNKKLKMTKSYHAVLAYDRNI
                     SKVFRIKTEVYYQYLFNAPVEKRPTFFSVLNEGADFGIAGIDSLENKGTGYNYGFEFT
                     AEKFYSKGYYFLFTASIFESKYKGSDGIERSTAFNSNFVFNLLGGKEWTIRNKNTISI
                     NIKTTYAGGRRYIPIDFAASQIAGETEYDEDHAFEHRRKDYFRTDLKIGYRINRKRLT
                     HEISIDLNNVFNTQNIWNQQYSAKTNTVKTEYQMGFLPIPMYKCTF"
     gene            43974..45767
                     /gene="mutL"
                     /locus_tag="PKY09_01220"
     CDS             43974..45767
                     /gene="mutL"
                     /locus_tag="PKY09_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNB11500.1"
                     /translation="MADVIQLLSDTVANQIAAGEVILRPSSVIKELMENAVDAGATRI
                     DVSINDGGKNLIRITDNGCGMSETDARLCFERYATSKLRKADDLYAIRTMGFRGEAMA
                     SVAAVCQVELKTKREADETGTRIEIEGSEFKLQEPCVMQSGTIITVKNIFFNVPARRN
                     FLKATPVEARHVMDEFLRVALPRPEIGFSLQHNGMEVYNLPATHQRQRICQIFGAAYN
                     ERLVPVEEDTTILNIKGFVGKPEFAKKVRGEQYFFINKRFVKDAYLNHAVTIAFNELL
                     PQGTFASYFLFFEVDPSRIDVNIHPTKTEVKLEDEKSVYAILRSSVKRALGRFSIAPT
                     LDFNQEQAFNVPLSQLNTIPVQPQVKVNTNYNPFKSVEINKPTQQQNGQWEKLYEIAG
                     LTDVQQTKLPIAPVEENSASAFMQLFNSYIAVAMQDELWLIDQQLAHEKIVFEKTLLQ
                     MKGTPTVMQQSLFPLQIELDNRQNATISSLLEDLNHSGFDLQPFGQNTFVLNAVPSYL
                     ETGTEKKIIEMLIDEFISGEASAYNPMERVAIAIARSACLKRGKVLSAREMSVLFNDL
                     MQCEMPYFTKSGKAIIFKLEKNEIDRKFEKG"
     gene            45874..46713
                     /locus_tag="PKY09_01225"
     CDS             45874..46713
                     /locus_tag="PKY09_01225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB11501.1"
                     /translation="MQEFRPSSFKLLPDVIKNIMIVSALAYFADLALNKIGFDLNAYA
                     GLYLPASENFHWWQYFSYMFLHGSLTHIFLNMFAFWMFGGILENLWGPKRFLIFFLVT
                     GLGAALIHTAVGYYELHSLQAKADAFFNAPGADAFSIFIKDNIPYQYLNADYAMAVEG
                     IKSNWYAHGDSPMIIEQARQAIDEYIRFKTNIPTVGASGAVYGVLMAFGILFPNTLIY
                     LYFFIPLKAKYLVLIIGGIELYSAWQSNPTDNVAHFAHLGGMLFGYLLLRYWQKNNRS
                     QLF"
     gene            46728..47600
                     /locus_tag="PKY09_01230"
     CDS             46728..47600
                     /locus_tag="PKY09_01230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB11502.1"
                     /translation="MKSNYKSLDMLQKIIVVNAIIYLFVNLGDVLLRLFNFPDGIISG
                     FLTDWLAVPSSVKSLLYRPWTLITYSFYHENFLHILFNMLWLFFMGRLFTEYLGEKKL
                     LTLYLVGGLCGSVLFIVFYNLFPLFSREVDIAVALGASASVLAITVAIATLLPDYEVG
                     LFLPQWRVKLKYIAIALFVIDLLSITGNNAGGNIAHIGGAIFGFIYIKQLKNGRDLTG
                     WLQRLIERITQKKKPSIKVVYRKDASDESYHNKKANNQEAIDKILDKISRSGYASLTY
                     EERDILFKASKNQD"
     gene            47600..48691
                     /locus_tag="PKY09_01235"
     CDS             47600..48691
                     /locus_tag="PKY09_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNB11503.1"
                     /translation="MLKSALNLLLWGITITIAIVTFMAVAAAYISPAKIWFLALFGLL
                     FIYLYLIAFIWMLTLSFRHRKMMLIILIPLIAGLPAFVAHWNFTSSNKNATGENTIKV
                     LSFNVRVFDLYNWTHNLETRSNIFNFLKKSDAGIYCFQEYYTSKLVPFNNTDSIKSLL
                     GIAYVHTLLPIIERDSDQWGIATFSKYPILNKETVFTDKESANGCIATDLLIGKDTVR
                     VYNIHLQSVRLAKNDYIFVSEFNNKDNTQKMKGSRQIVQRLKKAFIKRAHQVDKVTAH
                     ISACPYKVIVAGDFNDTPASYAYNQISNGLTDSFLSCGKGLSSTYAGMFPGLRIDYIL
                     HSKDIKSIDYIRPLVHLSDHYPIISTLNF"
     gene            48804..49538
                     /locus_tag="PKY09_01240"
     CDS             48804..49538
                     /locus_tag="PKY09_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HNB11504.1"
                     /translation="MRVKISFTRDNTSSNSIPLHHQKLIYDSLKTVVHDVGGNMKQLN
                     FSSLKGTSKIQNGFMKFLSSKITLVISGSNDELITNISNRIFEKNFMPIGRMNLLPKG
                     KEVTPDPEFTTQMRYLCISPLVLFDPIKDAARSIEQIDPTSHNFSDILFNTTLDAMEK
                     SGVPEATISSYAEFDVQPDAEYMQKAAAANKKFARVYRSAEGKNMIGYLVPFTLHAHP
                     DVHRYVWHNGIGVMTHEGYGMLDLVK"
     gene            49602..>50251
                     /locus_tag="PKY09_01245"
     CDS             49602..>50251
                     /locus_tag="PKY09_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11505.1"
                     /translation="MIKKYFIIILAVFVVVAGGIMLLRESIVRNKVNSFCTSFHTKTG
                     GNINIGKIQFKGLRVIEIENISLVSPLNDTLLNTDTFIVQLSFLKLIRLKNPVSNFSA
                     HGIKIKIASGDSISNYSFLFRKSKTENTETSTAQSTQASKVFTLWNKLTDLSDLSFDL
                     ERCKITIIRNKETRNFYFPEIINHRGNCRFIAYSNDHLQTEKWQAGFKIDDDKNELS"
     assembly_gap    50252..50351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <50352..51370
                     /locus_tag="PKY09_01250"
     CDS             <50352..51370
                     /locus_tag="PKY09_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNB11506.1"
                     /translation="TINKIHFKTKAAYDRKQPHRYALSLAFDESAADFFESLPKGIFY
                     HFKDFKAKGRLKFDLNFALDMSAPDSLIFNSGLTGNQLTIEKYGDENFSRINNTFLFD
                     AYDGDKLQRSFLIGPENSDFTPLNEICHYLQYAVLTSEDPSFFYHRGFIPDAFKESMI
                     ENIKAKRFVRGGSTISMQLVKNVFLSREKTISRKLQEMLIVWLIENKGLVSKERMFEI
                     YLNAIEWGPGIYGIKEASQFYFSKLPSQLNLAESIFLASIIPRPKYYRYSFDEQGNLK
                     PYLASYYKIVSNRMLSKTWISPIDTFNLQPQVKLKGPALKIVMPADSTITDSLQWNQS
                     NELF"
     gene            complement(51377..51895)
                     /locus_tag="PKY09_01255"
     CDS             complement(51377..51895)
                     /locus_tag="PKY09_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNB11507.1"
                     /translation="MKLLLIMFAFLFGPASTATSIYEFKVDGLSGGQIDFSQFKGKKI
                     LIVNTASKCGYTPQYSDLEKLFEKYKDKLVIVGFPANNFLWQEPGSNNEIAEFCKKNY
                     DVTFPMAAKVSVKGRDMHPLFRWLIAQPNADFTGGIKWNFEKFLIDENGKLIHRFRSS
                     VLPTSPEITKYL"
     gene            complement(51941..53248)
                     /gene="rimO"
                     /locus_tag="PKY09_01260"
     CDS             complement(51941..53248)
                     /gene="rimO"
                     /locus_tag="PKY09_01260"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNB11508.1"
                     /translation="MRTKTSKQLKVNVVTLGCSKNVVDSELLMGQLRANEIEVEHESK
                     RQSEIVIINTCGFIDNAKQESIDTILHYAKEKEKGKIEKLIVTGCLSERYKPDLEKEI
                     KGIDNIFGTRDLPLLLKTLGADYKHELIGERLVTTPRHFAYMKISEGCDRPCSFCAIP
                     LMRGNHISKPINELVQEAKQLAAKGTKELLLIAQDSTFYGLDLYKERKLASLMEQLCT
                     VDGIEWIRLHYAFPSGFPIDVLDVMQRENKICKYLDIPLQHASDNMLKSMRRGITRDK
                     TIALLDTVRNKIPNIAIRTTFISGYPGESEQDHETLIRFLEEMQFERVGVFTYSHEEN
                     THAYTLNDDVDETTKQRRLDELMQVQQNISDNINQKKVGKIFKTLFDRKEGNYFIGRT
                     EYDSPEVDNEVLVDAKENYVRVGDFANIKITSAEAFDLYGTLC"
     gene            complement(53252..53755)
                     /locus_tag="PKY09_01265"
     CDS             complement(53252..53755)
                     /locus_tag="PKY09_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11509.1"
                     /translation="MLSPFKILLAVFVLFSMSIQAQRIDKSFRLKVSDNRNLQFTSYE
                     YLLKDDSMKISGVSDYGKSRVVYIAKKLKSKQKKAIRNFLKDFPVDSLQKEYFDDYVN
                     FGYISSDHFPRVIELEIRTGLKQYFSKASNAYVKSYARLIDFLNTLINDDVVKINYME
                     TDFKKTF"
     gene            53971..54729
                     /locus_tag="PKY09_01270"
     CDS             53971..54729
                     /locus_tag="PKY09_01270"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNB11510.1"
                     /translation="MSFRNILLVAAIVFSSCKQSAHLVKTETTTYPLNKPGYTEVDSS
                     ILNSYSTYKHTIDSIMDRVIIVSEMSMQKKTPEGLLGNFTADAALKQVENNFHSKDFS
                     KIDFCFLNNGGLRAALPKGDITIGNIYSLMPFENEMVLLTLNGATTLKLINFIAAKGG
                     MPVSGLTMHIQDSKVTEAKIQGMPFDSTKSYVVATSDYLANGGDNLSFLVDAEKKIVT
                     GLKLRDLLIQYLEEKNKKGEKLSAKIEGRIIYDK"
     gene            54719..55654
                     /locus_tag="PKY09_01275"
     CDS             54719..55654
                     /locus_tag="PKY09_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HNB11511.1"
                     /translation="MTNRRDFLRTFAVGAAGLSLTGLPLEVLAKRKVTKLTILHTNDV
                     HSHIDPFPANDPKYPGLGGVAQRAAIINDIRNKERNVLLLDAGDIFQGTPYFNLFGGE
                     VELKLMSQLGYDAGTIGNHDFDNGVDGLSSQLKHANFPLIICNYDFKGTSMEGKTIPY
                     KIFEKEDVRIGVFGLGIELEGLVDKKMYGETKYLDPLEKAAETAHHLKFNEKCDLIIC
                     LSHLGYRYPNKKVSDDVLAKKSLNIDLIIGGHTHTFIDKPFVYKNRDNKDVLVAQVGW
                     AGIKLGRIDFFVDKKMRKLTADSTTLKVLNYARAI"
     gene            55817..57265
                     /gene="dnaA"
                     /locus_tag="PKY09_01280"
     CDS             55817..57265
                     /gene="dnaA"
                     /locus_tag="PKY09_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNB11512.1"
                     /translation="MLKQTEKNVEKVWENCLKLIRDNVSPQNFKTWFEPIKPVKLEKH
                     VLTIEVPSQFVYEWLEEHYIQLLRKSIKKELGAEGRLEYSILMENNMNSSKPYAVKIP
                     AKGTGNLKNPQVAMPVSLNTAIKNPFIVPGLKKIDVDSQLNPNYSFDNFIEGDCNRLA
                     RSAGYAVANKPGGTSFNPLLLYGGTGLGKTHLAHAIGIEIKNNFQNKTVLYVPADRFM
                     NQFVDAVKNNSPNDFVHFYQMMDVLIIDDIHFLAGKERTQDVFFHIFNHLHQNNKQII
                     LTSDKAPVDLQGIEQRLLSRFKWGLSADLQTPDLETRIAILKRKMYNDGIELPTEIVE
                     YIAYSITTNIRELEGALISIIAQTSLNKKPITLELAKQMIDKFVKNTVHEISIDYIQK
                     IVCDYFELPLETLKSKTRKREVVQARQLAMYFSKSLTKSSLSAIGAHCGGKDHATVLH
                     ACRTVNNLMETDKKFKGYISDLQKRINLNHKN"
     gene            57279..57740
                     /locus_tag="PKY09_01285"
     CDS             57279..57740
                     /locus_tag="PKY09_01285"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNB11513.1"
                     /translation="MVCLGNICRSPLAEGIMQHKLNQAGIAAHVDSAGLINYHSGELP
                     DKRSIAVAAKYGIDLTAQRARSFTKDDIKNFDYIFAMDNNNYENLMEYANDATEAKRI
                     HLILDYAGLGKKIVPDPYYGDQSDFEHVFSLLDDACQRILKKFAHAQSEVV"
     gene            57718..58452
                     /locus_tag="PKY09_01290"
     CDS             57718..58452
                     /locus_tag="PKY09_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNB11514.1"
                     /translation="MLNPKLSDIKTKGVLYLIPTAIGSMPAIDYLPQTVIETIHSLDE
                     FIVENSKSARQFLKYINIPTPQQQLIIHELNKHEGNVFETTFFSNLLSGKNVGLLSEA
                     GCPAVADPGALIVAEAHKLQIKVVPLTGPSSILLALMASGLNGQQFAFNGYLPKEKTE
                     RNAAIKKAEKDSAQRRQTQIFIETPYRNNALFDDLLNNLQPSTRLCIASALQCENEMI
                     KTQRVSEWKKQRQELNKHPVVWLFLA"
     gene            58463..59134
                     /locus_tag="PKY09_01295"
     CDS             58463..59134
                     /locus_tag="PKY09_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11515.1"
                     /translation="MVALLNFLLITIISCLVVSLILLMVSPRIPLFISYLAIALHILS
                     IALYLIYRNSIHEERVVMLFALIICSGIIPFTLIVRRNFPIILKIYFGIFMLSLPLFI
                     IAPSRMLTILSMGYLNTENAHEMLLDKNYYLLKEQGLIKQNDNEATYKVVKRMGIFNK
                     TLARNIFIGFRPDSAKILFLDEKSEIRLRAFKYNKTEIDSLDITTKTVSSRDTILKII
                     KTQHK"
     gene            59131..60096
                     /locus_tag="PKY09_01300"
     CDS             59131..60096
                     /locus_tag="PKY09_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11516.1"
                     /translation="MTRLFLSVFAIISVLTASAQQMSTDIFLTSFGMNREGQYFFTAP
                     QNITHRPGYDNQPSFSKDGSKVYYVAYYDTLQSDIYVYNLEDSSNTRLTETPESEFSP
                     RVTADDLGFTVVRVDADKGQRLYKILMDGTNEFQLLGVSDSVAYYCIANDSTYAVADL
                     NNNVMELNIYEMPGEQFIPLAKNIGRCIAVIPDGDNEISYVDKADTNGYMLMSFSINS
                     GLIGSVCQLKKGVEDYVWTKDGKLLIGYEGKLMMFDRSKPENGWTELADFSKSVGNFY
                     RIAISQQGTQLALVAYTKEDKKQDATKDDTGKKDEKKKRKKKKDE"
     gene            60197..61237
                     /locus_tag="PKY09_01305"
     CDS             60197..61237
                     /locus_tag="PKY09_01305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB11517.1"
                     /translation="MKTDFIIVGNGLAGSLLAWQFLLQEKKVVIIDKFNNSVASQVSA
                     GIFLPITGRRIVRTWMADELLAEVKTLFTELGKLNGETYLHEMPVVEIPASVKEWNEW
                     QQRASSPDISKYISRFLEPNAIESIHCPYGAIELKNSGYLNISKCVNFLRKLYLDKEI
                     LIEEKFNFANLQYSDKINYKDVKADKIIFCEGAEGVNNPFFNYLPFQLSKGEILTISC
                     NTLSEKQILNHGIFILPIGNQLFRVGSTYEWNDLTTNTTESARNNLNEKLQAVLKTDF
                     EIIEHKAGIRPSVKERRPFIGMHPKHKNIGIFNGLGTKGVMLAPYFSKHFAWHLAHEK
                     PLMADVDIARFS"
     gene            complement(61239..62414)
                     /locus_tag="PKY09_01310"
     CDS             complement(61239..62414)
                     /locus_tag="PKY09_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNB11518.1"
                     /translation="MPSVSFNNLKVAFAYKSDADLKRAYWLFRLININFLVKIGPGFT
                     NAVLKMGLPIKPLIRATIYKHFCGGETIEQCTETINNLGKYGVGSILDYSVEGHHNES
                     DFDHSCAEIIKTVKHASGKKNIPFSVFKVTGVGRMDLLERVSSKVLLTGAEDAEFQRL
                     KARVESICKTAAENNVRLFIDAEETWIQDAIDQMVDDMMLKYNKEKTLIFNTVQLYRH
                     DRVAFMKENIAKARSGNYKIGYKLVRGAYMEKERERAALNGYPSPIQPDKAACDKDYN
                     EAIKLCIENIDMVAVCAGTHNEESSALLAKLMEEKGIANSDERIWFSQLLGMSDHISF
                     NLANAGYCVCKYVPYGPVVSVLPYLFRRAAENTSMSGQMGRELSMITQEIERRKKEK"
     gene            complement(62616..63413)
                     /gene="cdaA"
                     /locus_tag="PKY09_01315"
     CDS             complement(62616..63413)
                     /gene="cdaA"
                     /locus_tag="PKY09_01315"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNB11519.1"
                     /translation="MELFHIGFLTVRLLDIIDVLIVAWVLYKLYRLLKGGVAMNILIG
                     ILTIYFLWWLFVKVLDMQLLGALFGQFIGVGVIALIIVFQQELRRFLIFVGSNTIFTR
                     GDFFKNLLHGNFKLQQPVELSVMPIVRACVSMSKTKTGALIVVTRKTDLNLFEGTGDF
                     MEAEVSKRLLESIFFKNSPLHDGAVIISGNRIRAARCVLPVTESLDVPAHYGMRHRAA
                     LGITEQSDAIALVVSEENGQISIAAGGQFYSNLSPDELERKLKELME"
     gene            complement(63543..64778)
                     /locus_tag="PKY09_01320"
     CDS             complement(63543..64778)
                     /locus_tag="PKY09_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB11520.1"
                     /translation="MELKELLLWIIASILVLNYLLSMSLSILNLLNLSETQPDELKQY
                     YDEERYKKSQQYEREKSRFGMISETVMFIISFFFLILGGYGWLDGWVHSITTDLLWST
                     LLFFGTFALFSDLLSIPFSLYSTFRIEARYGFNKTTLAVFISDRIKGYFLAGALGGSL
                     LALFVLFYQYTGSDFWIYMLAVFAIFMVVLNMFYTSLILPLFNKLTPLAAGEVRTAIE
                     NYCHENKFEVANLYVMDGSRRSSKANAFFSGLGGKKKIVLFDTLVNNYSKDELVAVLA
                     HEVGHYKKKHTLFSMIFSILQTALMLWLLSVFINNEWFSLALGGTEYSLSISLIAFTL
                     LYSPLSLITGILMNIYSRKNEFEADNFARVTFSAEALASALKKLSSDSLSNLTPHPMY
                     VFFYYSHPPLLQRLRALQT"
     gene            complement(64769..65887)
                     /locus_tag="PKY09_01325"
     CDS             complement(64769..65887)
                     /locus_tag="PKY09_01325"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNB11521.1"
                     /translation="MIHKAGISFLSTLFLFMLTLTLHGQQVTHGSWMLGAEGHYGFVM
                     AHRPSVQPLQHNHLGGMMITLSHQADGKRSWHQHFHFPETGIKYGFFGLGNKEQLGYG
                     HTVYPYIDIPLGKSQKPFLHFQFGWGLGYITKPYDRNKNYQNVAIGSHLNAVIAFNFH
                     SEFWLTKRDRIVPAIGLTHFSNGSAAMPNLGINLISAQLHYSHSFGESKKIISHEPVN
                     FEKRNRFTIFSAWAFKQVYPLAGRTWYAWTLSPAFLRQYSPKSAAGISVDMFYDPTIN
                     YRLEYEDMKTQGSISNFRGGVAGAYEIIFSDFSVVLQMGVYAKNAWKKDGLFYNRLGM
                     RYCFYDGLFACINLKTHIARADFIEWGIGYKFKNKAWN"
     gene            65879..66700
                     /gene="mazG"
                     /locus_tag="PKY09_01330"
     CDS             65879..66700
                     /gene="mazG"
                     /locus_tag="PKY09_01330"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNB11522.1"
                     /translation="MNHTFAKKMLLAMEEKLNAFKRICTIMDELRTQCPWDKKQTIES
                     LRHLTIEEVYELSDAILDNNLEEVKKELGDIMLHLIFYSRIAEEKNLFNIADVINTEC
                     EKLISRHPHIYPSADGKKIAVADEEEVKRNWEKLKMKEGKQSVLEGVPRSLPSMVKAQ
                     RIQEKARGAGFDWEKPEQVWEKVEEELNEFQEAVSRKDQKDMSDELGDVFFALINYAR
                     FHNLNPDEALERTNRKFISRFRYMEDKVRESGKTLHELSLAEMDVYWNQAKTTEI"
     gene            66703..67389
                     /gene="trmD"
                     /locus_tag="PKY09_01335"
     CDS             66703..67389
                     /gene="trmD"
                     /locus_tag="PKY09_01335"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNB11523.1"
                     /translation="MHIDIITVHPGLLEGPFSHSILKRAIQKNLVEINIINLRDYATN
                     KHKSVDDYAFGGGAGMVMMIEPIDHCIADLKSKRTYDEVIYMSPDGEQLNQGISNSLS
                     LKKNIIILCGHYKGVDERVRQHLITREISVGDYVLSGGELPAAVLCDSIIRIIPGVLN
                     DETSALSDSFQDNLLAPPVYTRPANYKGWDVPEVLLSGNESLINEWRHLQSLERTQIR
                     RPDLSDDNSK"
     gene            67515..67886
                     /gene="rplS"
                     /locus_tag="PKY09_01340"
     CDS             67515..67886
                     /gene="rplS"
                     /locus_tag="PKY09_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNB11524.1"
                     /translation="MDLVKLAEKQLIPVKDTPDFKAGDTITVFYKIIEGNKEREQQFQ
                     GVVIQRKGHGATQTFTVRKISNGVGVERIFPLYSPKITKIDVNKRGVVRRARIYYLRN
                     VSGKAARIEEKIVKTGETKKK"
BASE COUNT        22684 a        12575 c        12143 g        20428 t
ORIGIN      
        1 ttaactaatt atggaggata tattttataa tattttgtgg atagatgatg agcatgaaag
       61 cttgacaagc actaaaggaa gagcgaagag caatggaata aatctcattg ggtttaaatc
      121 attaaattct ggaatgagtg aactcgaaag aaatctccac ttttatgatg gcattctatt
      181 agatgctaaa ttttatgaaa atgatgatgc tattgctggt tccgaagaca cagacaatgt
      241 tcatcgtgcc aaagagagac tacttcagct taataaaaag tttgaaatat tcgtttacac
      301 aggtcaggca gaggcttttg aagataaaac ctttaacaag gccttcaaaa gagtctacaa
      361 gaaaggcagt tctgatgaac gtgatagact attcaatgac attaaacttg ctgctgcaac
      421 gcaggaagac acccaaattc gccacagtta taaacgagtg tttgacgtat gcactgaaaa
      481 gtacattggt gaacttgctg gacaagatat tttgaacctc cttaaagtca acgatgaaac
      541 caatattgac aatcatttca acgcaatacg aaaagtagtc gaagaccttt ttattgcatt
      601 caataaattt aatttgcttc ctgctgaatt tgttactcct ggggttgctt tgaatgaaag
      661 cagtaagttt ttggcaggca aaggatctga tggttctctt ttttccgaaa aaggatatca
      721 gcatttataa gaaacacatc tatcgaaaca aattgccctc tacttgcgca gcattttgtc
      781 agtaacacag gcaggctcac acagatcaga aattgacgta cacgtaagaa ctatcaaaac
      841 tccgtatctt ctgaaaagtg tattctttca gttattggat gtgctggtat ggtttaaaat
      901 gtatgttgat tccaatccaa aaaccgagaa ttggttaaaa gtggaaaccg ctgccgaagc
      961 agctgaacca gcggctgaac tcattcatgg caaggttatc aacattcatc cccaaaatgg
     1021 ttttgcattt ttcaaacctg atgccatcgg tgataatatt ttcatcccac cgcatttggt
     1081 cacaaaccat tcgcttacag aaggaatgcc cgtgcaagtg gaagtggagg aatacaccga
     1141 caaccgcacc aacgaattaa aaaacagagt taaacgaatt gaaatgcgac cataaacacc
     1201 tatgacattt tgcaagcaca tcgccaaagc cgcacatgcc taccagcaac ccaagcttgc
     1261 aaaagagccg ttttttgcca acgcactgac aaaaaaccgt attttggaca attaaattat
     1321 ttagagatat aaatgacctt taatgaagat tcaagagtaa aaattcctgc aatcgtgcat
     1381 ctgacacggc tgggttactc ttatttgcct aagtcagcaa ttgcaaacct acacaccgac
     1441 accaacattt tcaaagacca gttcaaggca ggactttcta aaatcaacga cagggaattt
     1501 accgacaccg aaattgattc cttcctttca gaaatcaaca ttcaacttga caacagcgac
     1561 cttggcaaac gcttctacaa aagtttgctt ggagattttg cctgtaagtt aattgacctt
     1621 gaaaattttg aaaacaatct tttccatgtt gtaaccgaac ttacctacaa aaacgaagag
     1681 gaagaattca gacctgacat cactttgctg tttaacggaa tgccgttatc attcatagaa
     1741 gtgaaaaagc cgaacaacag agaaggaatt ttagcggaac gaaacagaat aaatgctcgc
     1801 ttcaaaaatc cgaagttcag caagttcatg aacattacgc agttgcttgt gttctcaaac
     1861 aatcaggagt atgacgagga aagtatcaca cccatacaag gtgcgtttta cgcaacacct
     1921 gaccccgaac aggtaaactt caattgcttc agagaagaag aaactgcaat cattcacagt
     1981 gtgccagcag aagattcaga aattgaaaaa caaattttgt tggacaacaa cattgtttcc
     2041 attcttggca attcagaata caacacagca aaagataaca actcccctac taatcgtgtg
     2101 ctgacttcgc ttttcagcaa gcagcgttta aaaacaattt tgaaatatgg ttttgcgtat
     2161 gtgaacagtt ccgacaaaat cggaaaccga atggagaaac acatcatgcg ttatcctcaa
     2221 ttgtttgcga ctttggcgat tgagaaaaaa ttaaatcagg gaatcaaaaa aggaattgtt
     2281 tggcatacgc agggaagcgg taaaaccgct ttggcgtatt acaatgtttc ctatctcaaa
     2341 gattattttc agaagcaaaa caccgttgca aaattctatt tcattgttga ccgtttggat
     2401 ttagcacagc aagccaaaga agaatttgtt gcaagaggtt tgacagttga aatgataaat
     2461 tcaaaagagg atttcatcaa taacattaaa accgcaggtg caacggcaac aaatgttgga
     2521 gtgcaaacga taaccgttgt caacattcaa aagttttctg attattccat tgccaatatt
     2581 tccgactaca acctgaatgt aaaacgcatt tactttttgg atgaagttca ccgtagttac
     2641 aatccagaag gaactttcta tgagaagtta gtcaactccg acagagaagc tattcacatc
     2701 ggactttcgg gaaccccttt aatcactggt gatttcaaga gcaaagaaat ttttggcgac
     2761 tacttccaca aatactatta caacaaatcc attgcagacg gttacacttt gaaactgatt
     2821 cgtgaggcga ttgaaacaaa attcagaacc gacatcaaca aagtgtatga tgaaattgta
     2881 aagcaaggtg agtttaccaa gcgtgatgtt ttttctcaca agaaatttgt tgagccattg
     2941 gttgattaca tcattgccga cttcaagaaa agcagagtga ttcacaatga taattccatt
     3001 ggaggaatga ttgtttgcga ttcatccgac caggcgaaaa tgatttttaa atttgtgcag
     3061 aaatacaacg atgcaaaaac aaaacttcgc caactgacaa ccgaaccgaa cacacaatac
     3121 actattgcag cagaactgaa agaagaatat ctgaatgctg aagatgcacc gataatagcc
     3181 gctttaattc tgcatgatgt ggacaccaaa caaattcgca aagacaacca aaccgatttc
     3241 aagcaaggca agattgattt gctgattgtg ttcaatatgt tgctcaccgg ttttgatgca
     3301 aagcgattga agaaacttta tctcacaaga gtagtgaaag aacacaacct cttgcagacg
     3361 ctcacacggg tgaaccgacc ttacaagaat ttcaaatatg gttatgtggt tgactttgcc
     3421 gacatcagaa aagagtttga caaaaccaac aaagaatatt tcaaagagct ttcggctgag
     3481 ttaggcgatg aagcaaaaga atattccaac ctgtttaagt cagcagaaga aatagacaga
     3541 gagattgcgg agataaaaga gaaattattt ctctacgact tttcaaactt agaggagttt
     3601 cagaaaatca taagccggat aactgacaag aaagaaatta ccgatctgaa aaaatgtctg
     3661 gagaatttaa agagtttata caacatcatc aggataatgg gttacaccga actgttggac
     3721 aagttttctt ttgacaaggt gaacaagttg tataacgaag ttgcaaaccg cattgacatc
     3781 attaacctga aagaaaatct tgaagacgca aacgacaaca caaacttgtt gaacattgca
     3841 ttggagaaaa tgcaattcac ttttcgcaag attcacacac acgaattgca gattgcagac
     3901 aaattcagaa gcgagttgga aagagcaaga aaagaattgg aaggaaactt tgacaagaaa
     3961 gacccaaagt ttatttcctt gtttgaggaa ctgaaacgat tattcaaaaa gaaaaacatt
     4021 gaggaacttg attcggcaga aatggacaca gctataaaag atttgaaaaa catttacgaa
     4081 caggcaaacg cattgaacaa taaggatgca cagcttgcag ccaagtatga gaatgatacc
     4141 aagtttgcca agattcacaa acgcatcagg gaaaataatc tgcccgtgct gaataccgat
     4201 accgaactgc atgaagtgct gcttaccatc aaacacaaaa cagataaaac cgttatcaat
     4261 aatcaggcaa tattaaacaa cgaggacttt tttacagaag ccacgaagcg aacgattatt
     4321 gaagtaatgg aagaaaaagg aattagaaat ttagagttgg tgaaatttat taaccaaaca
     4381 ttagttaacg aatattttga acaacgcaaa gctgcatagg catgagtaat attcagaaag
     4441 ataagctgtt tcaatccgtc agggaaatcg ttctggaagc acgaaaaaat gtttaccgga
     4501 gcagcaatac tattttgttg aagatgtatt gggaaatagg caggctaatc gttgatgatg
     4561 aacagaaagg tgcaagccgt gctgaatatg gcaaatcagt tttgaaaaac cttgcccggc
     4621 aacttaccct tgaattcgga aaagggtatg atgaacgcaa cctcaataac atgagggctt
     4681 tttataactc atttcaaatt tggaacgcag tgcgtaccga attaagctgg acgcattaca
     4741 ggattataag caggttggaa aatgcggcta accgcatagc ttatatgaac gaggccatag
     4801 caggtaattg gaatacccgt accctgcagc gaaacattga cagtcattat ttggggcgac
     4861 tactggaaac accgggagca acaggctctt caacgcctga acactatatt aaagaccctt
     4921 acatttttga attccttggt cttcagcaag acagtacatt aagtgagacc gtgctggaaa
     4981 cagccctcat caatcatata caaagcttcc taatggaatt gggcaagggc ttcgcatttg
     5041 ttgccaggca acaacatatt gcaaccgata cttccgattt ttttattgac cttgtttttt
     5101 ataattatta tttaaaatgt tttgtgttaa ttgacctcaa aacaaataag ctcagccacg
     5161 aagcaatcgg gcaaatggat atgtatgtgc gtatgtataa cgatttgaaa aaagcggaag
     5221 acgacaatcc tactatcggc atcatactct gcaccgaaaa ggatgaaacc attgtaaaat
     5281 attccgtact tgccgaaaat gaaaaacttt ttgccagcaa ataccgcctc tacctaccga
     5341 aagaagaaga actaaaagaa ctgattgaaa acgacagaac aaaatttgaa ttagaaaaat
     5401 gacaaagcat ttagaacata cacagaaaac aaaagaactg attgacggac tgaaaaccgt
     5461 ttgcagcaat tacggattgg caaacacggg ttacgaatat aaaatcatta cagaagtttt
     5521 tctctacaaa tttttgaacg ataaattttt gcatgaagca cgaaacgcca acaagaaatt
     5581 aaaagacagc aaaaacattg aagccgacat ccaaagcatg acggacaatg attacgaata
     5641 catgctggag aaaataggtg aacgaagtgc gaagctgaag aaaacacatt tcatcagtta
     5701 cctgttcaac aaacaaaacg aaaaagattt ctccatcacc tttgacacaa cgctaacaga
     5761 aattgcagcc accaacatca aaatattttc tgtttctact attggcggag aaaggattcc
     5821 gttgtttgaa gaactatctg aaaacataag agagaaaaac gaaagaagtc cgttctgcaa
     5881 agccattatc aacaaattgg tggcgttcag ttttgaaccc gtgtttgagg agaagtatga
     5941 tttcttctcc accatttttg aatacctgat taaggactac aacaaagaca gtggacaata
     6001 tgcagagtat tacacaccgc cagccgttgc aaaaattatg gcacgaatac ttgtgccaaa
     6061 ggaaaccaag aatgtgttgc tttacgaccc agccgcaggt tcaggaagtt tgttgttgag
     6121 tttggctcac cagattggag aggacaaatg caccatttat tcgcaggaca tttcacaaaa
     6181 gtcgtcagag tttttgcgat tgaatttaat cctgaataac cttgtgcatt cgctgcacaa
     6241 tgtgattaag ggaaatactt tgacaaatcc ttttcatgtg aacgatgcaa aggatgattt
     6301 gaaaaagttt gactacatcg taagcaatcc gccattcaaa actgatttta gcgatgacag
     6361 ggaaacactt gcaagcgata ttcacaagaa acggtttttt gcaggagtgc caagtgtgcc
     6421 tgctaagaaa aaagagagca tggcaattta tcagttattc attcagcaca tcatgtattc
     6481 gttggacaag aaaggaaaag cagcggttgt agtgccgaca ggtttttgca cggagaaagc
     6541 agccattggt ttaggcatca gaaaaaaatt agttgacaac aactggttac atgcagtggt
     6601 gcaaatgcca tcaaacattt ttgctactac aggcaccaat gtttcggttc tgttttttga
     6661 caaaggaaaa aagaacaaca ccgccattct tgtggatgcc agcaagttgg gaacaaaagt
     6721 gaaagacgga aagaaccaaa aaactttatt gagtgcagaa gatgaaaaca aaattattga
     6781 cacaatcaat aattatgaaa cagttgaaaa tttttcaatt gtagttgaaa acgaaaaaat
     6841 taaaacaaga aattattctt tcagtgcagg gcagtttttt gaaactacga ttgaatactc
     6901 agaaataact caagaggagt ttgaaaataa aatggagggc tttgaaaaca atcttgactc
     6961 tctttttgaa caatccaaaa atttggaaga aagtattaga aaacagttat tgaaaataga
     7021 gtatgcacaa gctgacaaaa tataaattca acaagctgta tgaaatgagt tctggtattt
     7081 catcaaagcc agagcaagca ggacatggtt ctccatttgc ttcttttagc actgtattca
     7141 ataattattt tttgcctgat gaacttcctg atttaatgga tgtgtcagag caagagcaag
     7201 aaacatactc tatcaaaaat ggcgatattc ttcttacaag aacaagtgaa acaattgatg
     7261 aacttgctat gagttgtgtt gcacttaaag attttccgaa agccaccttt agcggtttca
     7321 caaaaagaat cagaccaacg caaacagatg ttacctatcc caaatacatg ggattttatc
     7381 ttcggagtaa ttttttcaga aggaccatga cgaacaatgc agtcatgaca ctacgggcaa
     7441 gtttgaatga agatattttt tcttacttgg atttattgct tcctgatttt gatactcaaa
     7501 agaaaattgg cgactttctc tatctgcttg acgacaagat taaaatcaac aaccgcatca
     7561 acaccgaact ggaagccatg gcaaaaacca tttacaacta ttggtttatt cagtttgatt
     7621 ttccgaatga agaaggcaag ccctataaag caagtggcgg tgaaatggaa tacaatgaga
     7681 aattgaaaat ggaaattcca gttggttgga ctgatggaaa gttgagcgaa gttgcaaaca
     7741 ttacaatggg acaatcacct gacggtgatt catacaatga agaaggagaa ggaatggttt
     7801 tctttcaagg cagcaccgat tttaattttc gttttccgct tgtgcgtatg ttcaccacag
     7861 caccttcacg gattgcacag gaagaagatg tgttgttaag tgttcgtgca cccgtaggaa
     7921 cattgaatgt cgcaaatgaa aagtgttgca tcggacgagg acttgcagca ttgaacagca
     7981 agaatgaagc aaactcattc gtgctttacc tcatgtttta tttcaaagca atgtttgatg
     8041 ttcaggacag aacaggaaca acattcggct caattgacaa agacactttg catgatttga
     8101 aatttgcaaa gccaccagtt gaattgcaaa aacaatttca aagcatagtc ggtaaatacg
     8161 ataaaatgat tttgacaaga agcagagaaa cccaagaact cataaccctt cgtgatttcc
     8221 ttttaccgtt gctaatgaac gggcaggtga agctggtctg aactatgatt tttgtgattg
     8281 aatgaatgca atgaaaaaat ttatgccatc aacaaatata tctaaaggat cggaatacag
     8341 atgttttaaa atcatagaaa tcaaaagaat caaatgaatc atagttaaga caacatgata
     8401 aatgaacaat acaaatattc tgagttgaca tccaaaatta tcggatgcgc tatggctgtg
     8461 cataaggcac tcgggaacgg ttttcaggaa gttatttatc aaagagcatt ggaaattgaa
     8521 atgcggttga tggggataga atttagcagg gagtttgaaa tgccaatttt ttactgggat
     8581 gaacagattg ggacaagaag ggtagatttc cttgttgaag gtattgtctc cgttgagcta
     8641 aaggcaatca ctaaactgga agatgttcat tttgcacagg caataaatta ccttgaagca
     8701 tataacctgg aaataggatt attgataaat tttggcgaga caagtttaaa tttcaaaaga
     8761 ctgaccaata aaaaattcaa accaatcaaa tgaatcaaaa aaatcatggt tcagacattt
     8821 gctgatgaac gggcaagcga agctgaggta aaaaaataaa actatggaca aagaaacatt
     8881 tacaagccag cccacaaacc aaagcttgca aaagagtgcg cctacccacc caagaagaat
     8941 ttactcacag catatttatt tttagtggag ttcgtgattg cttcgcagtt caaaatattt
     9001 tatccaacgc ttcaaaaaaa attaaaacac ccgacacgta aaccaacccg acaacgacaa
     9061 caaaactcaa caatgactat ggtttactgg ggcggacgac aagaaccact ggctataaca
     9121 agcagtttgt caaaatggcg gcagaagtgc tactatgaaa catttgtgct aaattcaacg
     9181 gtagttattc tattaaagtt cagtgctaaa aatcccccac tgcacaaatc ccgcccgtca
     9241 ggctgtgaaa aagccaattt ctcttaaaat attattacta aaattatcac acaagcattt
     9301 gtttacattt taagacaact tcatattgaa aatcgtcatg gataagtgaa agataaaatc
     9361 acttaacctt taacagtttt cttgtgctca cattctctcc tgaagcaatg gtcagaaaat
     9421 aaattcccgg gctcaatagt tctgtattta tttttgtctg cgcgctaatt atcgctttgt
     9481 aaattatatt acccaacaca tcagttaaaa ttaattgagt ttgtttaaag ttgtctgaaa
     9541 tatttatctg attaacgaaa gggttaggat atatcaacaa atcagctgaa ctcatattat
     9601 gcacagatgt tgttggaaac gcatatttaa caaagtaacc atcgcgtgag ccggcactca
     9661 ccaaggtgtt aattgcaggt gcaggattaa agtcaacaac ctgttcaaag ccaccacata
     9721 aaaacacttc gtcaatgccg ttacaggtca tgttgcggca aaactcaaaa gcagggctgc
     9781 cacatccaaa agcagaaaga taatttccca gagagtcata atgtgcaaga tacatatcag
     9841 caaaaccatt actattcaat gaggctacgg taacatccgg atcaaagtcg gcagtgttaa
     9901 aaaaatatcc actcaccaat attgagctat ttgaaagttc ggcaatggta taacaaatat
     9961 cagaaccatt tccaccaatt cttttagcaa aaacataatt accgccattg gtatattttg
    10021 caaaataacc atcaaagcta ccttgtgcaa tcaacgtcat tgtatcagca tcaggatcaa
    10081 aatctgcaaa gtttgaaaac tgtccacatg tcagaatatc accgttatga tcaactttca
    10141 atccaaacgg ataggcattt tctattggga aagaccaata gtagtttcca aaattactga
    10201 atgaggcagt aaacgctgta agtccgtcag caaaaagcgt atcgggattt acacctccgg
    10261 aatcaaaaat caatgtgtct tcaaatgtac ctgtaagata tgttttgcca ttgataccgg
    10321 cagtgatgtt gtaggaatga tcttctccat ttccttttag tgtattgagc caaaacaaat
    10381 ttccattcgg attaaatttt ccaaaaacca catctgcact tccgctgcca taaagtggag
    10441 taattgtcaa tccatcatca acataggatg aatctgcaaa tgagccagca aacaaaacca
    10501 aataattctt atagtcaata cggtaaacat catcgtttaa tgcaccacca acgcctctaa
    10561 cccattgcaa caatcctgtt gagtcatact ttgcaacaaa gccatcctgc tgacctctgt
    10621 tggttgctat cacagcattg cccttaggat taaaatccgt attgctatta aacaccccac
    10681 ataagtaaat attaccatac tcatcaacag cagggtctgt ataattaaac tcctctcctg
    10741 ccccaccaac atgaatcgcc caaacaaaat ttccggcact atcatatttg gcaagaaaaa
    10801 tatcattacc accattgctt gttaatgtgt agttagttgt gcccgggtca aaatctgcag
    10861 ttcctgaaaa ataacccgac acaataatat ttcccatact atccaatgca attcccatag
    10921 cattctcaaa ttgtgtgcct ccaacacgtt tggcaaactt atattgctgc gctttaatat
    10981 cagaagcaaa cagaaaacac aaaatgaata aaataaaaat aatcaggctc ttttgctgct
    11041 gcataacagt tacttttgtt ttatcaggta aagatatgca atcagccaat tgttgtctga
    11101 caactaaagc atatttttgt agaaactatg gagcattcaa caaaaacttt tgcactaaaa
    11161 aacggattac agcttgttct tacttcttta agcaacacag aagtttcaca ttgtgcacta
    11221 ctcataaaag ccgggacaag aaatgaacaa attcaacaag aaggattagc acatttcatt
    11281 gagcacatgc tgtttaaagg aacacgcaaa cgcaaatcat ttcatatttt aaatcgtctt
    11341 gaagttgtag gtggtgagct gaatgcattt accagcaagg aagaaacatg cattcacgca
    11401 tctgtaacta cacctcatct tgaacgtgca cttgaactta tcagcgatat caccttcaat
    11461 tctgtcttta atagtcttga gattgaaaaa gaaaaagaag ttgtttgtga tgaaataagg
    11521 tcatatctcg ataatcccgg tgagcagatt tttgatgact ttgaaggcat cgtatttaaa
    11581 aaaaatccat taggaaatcc aatcttagga acagaaaatt ctgtcagaaa ctttaagcag
    11641 aaagacctga ttgcatttac acgaaaattc tacataccat caaacatggt gctttctgtt
    11701 gcctcatcat taaaagcaga aaaagtaatc agacaattag aacatcattt cggcaagtat
    11761 aggcttgcca aggttggcga aaaaccaaaa ccttttaaaa aatatacggc tgaaaaaaca
    11821 gcagtaaaga aacacaatgc gcaatgtcat tttattatgg ggacattagc accttcactt
    11881 tatcaggcag acagaatgac tgtttctctt ttgaacaata ttcttggtgg accgggcatg
    11941 aactctaaac ttaacttagg tgttcgtgaa aaatacggct acacgtatgc tattgaatca
    12001 gggtttaact cctatacaga tacaggcatg tttcatgtct atctggcaac agataaaaaa
    12061 ttcctgaata aaagtattga tcttgcagta gcagaaatga aaaagcttac tgacaataaa
    12121 ataggaacat tacaattaag tcaatacaaa cagcaactga aaggtcaatt ggctttggca
    12181 aaagaaaaca aatcgaatgt agttatctct aatgcgcgca gtcttttaaa ttttaagaaa
    12241 gtaactacct tagaagacat atacaaaaaa attgatgccg taacttcttc gcagttggtg
    12301 gaagctgccg caaagtatct gaatgcaaaa tcgcgcagct acagtagcct gctttatgaa
    12361 agcgttagtc atgagtaaca atactgcctc atttttgaca cgcattgttg atgaacttat
    12421 tgcaacagtt gatggaaata attttgcaga ccatactgtc atatttccta caagaagggc
    12481 tgctttactt tttaaattac aacttgcaaa aaaactaaac aaagctattt tattaccttc
    12541 agtattatca ataagtgatt ttgttgaata ttacataccg ctgcgtgtct ctgaaaagaa
    12601 agaaatactt cttgatttat atgctgttta taaaccacat tttcctgatt atgattttac
    12661 atcctttgca ccatggggtg aaatgattct tagcgatttt gatgaaatag atctttactt
    12721 agttaattac aaagacctgt ttgcagacct tacatcctat catgaaatag actcggtttt
    12781 tcagggtgat gaagaattta aagaacagtt gaaaccgttt tttgaattgt tcatggcaga
    12841 taaaacttct acgctaaaac aaaatttttt atatgcctgg catgcattgc cgaagttgta
    12901 taaagagctg aatgaagtat tagagaaaaa caataagacc acagcaggaa aagcaatgaa
    12961 gcaattagct ttgtcgcctg aaatatttct gaaaaatctg gattgcagta agactattat
    13021 tgccggattt tatgcattgg cacctgctga acaaaagctg tttgaattta tcctaaatgg
    13081 aaaagggaaa atattctggg atgccgatgc atattacaca aataatacaa ctcaggaagc
    13141 cgggctcttc ttcagaaaaa atgttttaac tcagaatagc tttaaatgga aagaaacata
    13201 tttcaaagat gtacctaaga acattaatgt aaccggtgtg ccgctcatca caggacaatt
    13261 taagtttttg gctgatcgtt taaagacttt aattgctcag aaaaagttca atcctgacaa
    13321 gacagctatt gttttgcctg atgaaaaact actgcccttg atgttgcaat cattgcccga
    13381 agatattgaa catgttaata tcacaatggg gttcccatta aaagaaactc acctgtatcg
    13441 ttttatcgaa acattaaatg cattactcaa acaaattcct aacacttcta agcagaagtt
    13501 agttcctacc tctgcattag gaaattatct tgcacatcct tatgcttcat tattgaagca
    13561 caacatgtgc aatataaatg atgcaggctt aagtattcca ttgattgaac ttcctgaaaa
    13621 catcagtcag tttttaaaat tacagattac cactcccgtt ttcgagcgtt atattcaatt
    13681 gcttaccatg cttcatgatg ccatggaatc taagcatcat gaaaaagaaa ttgccggatt
    13741 tgttttaaat gagcttcttg aacttcgtac aatggtggct ccttatgctg aaattctgga
    13801 tgaaccatct ttgcagatgt taatttcgga aatgctcgac acaacgagaa ttccatttag
    13861 tggggagcct gttatggggc ttcagattat ggggttgttg gaaacacgca cactggattt
    13921 tgataatata ttttttctta atgtcaatga aggctctgtc ccaaaatcaa atcgtcacca
    13981 ttcatttatt ccatttacta tcagaaagag ttttgggctc ccattggtcg aagagcaaga
    14041 tgccattact gcctatcatt tctggcgatt gatgcagaga gcgtcagaaa ttgatttaat
    14101 ttacaacaca gaagttaatg agtttgccag tggcgaaaga agccgttatc tgttacagtt
    14161 gttttatgaa atgaaagcac agttcagcaa ctggcaggtc aatcatcaga ttattaccac
    14221 accgcttcat cgttacaatt acatttcccc cggaattaaa agagatgagc atctgacaaa
    14281 acatttactg tcaatggtgg ctgagaataa attaagattt tctgcttctt cacttaataa
    14341 ttttatttat tgttcgctaa agtattattt taatgaaatt aaaaaaatca gaaatccgga
    14401 tgatactgaa gaagaaattg acaataaagt attcggaaaa attttgcacg atgcagtcag
    14461 caaaatttat gctcagataa tagatgttga gtttgataaa aacattgcag cacaactcag
    14521 aaaatctatt tcaacatttg taaaagcatc tattgcccaa gaatatgata aacattatca
    14581 tggaaccgga tatactgtaa tcatagaaaa aataatcacc gaatacatcg agcgtatcat
    14641 agcatctgat ctgaaaggtg taactttttc tcctcatgaa atagaaacag aaaaagtagt
    14701 tgtttgtgat attggcaatg gcattaatgt aaaaattaaa ggagtttttg atcgtataga
    14761 taaagtttat gatggataca gaatagtaga ttataaaaca ggcggtgatg aagtaaagga
    14821 tgtaaaaaaa cctgatgatt taataacaaa tccaaactac aaggttaatt ttcagctttt
    14881 tctttattgc tggttgtttt taaaatctca cgataaagaa aaagttaatg caggaattta
    14941 tccactcagg cgaatggaag atggcttaaa aatgctgaag caggaacaaa tagcagatca
    15001 ttcaatgttt gaagaattgt tgtttacatt aatcagaaaa attttattag agtctgattt
    15061 tactatgaca acagacacca cacgatgtaa gcattgccct tacaaggata tctgcaacag
    15121 ataatcacat cagtaatttc tcaactatgc ggtccacagg aaatgttact acttcaccat
    15181 tgagttcttt caatggcttc cagaaaaaat gaatattatt tgcatcttca ggtaaaataa
    15241 aattctcgca taacactttg taataaacgc tgatgagttg taatccggga ttgaaagccg
    15301 attcaataaa aaaatccgct gtataaaaat gctctttaac ctctaccttt tgttgggttt
    15361 cctctaaaaa ttcgcgcacc aaacattcgc gcagaccttc accaaactca agtccgccac
    15421 ctgggaattt ggtaaattct attccgcctt ctttttcatg agtaagcaac acttctgaat
    15481 tcttatttat cagaattccg taaacccgta ctacaaatgg tttcatcgtt tcaaaattag
    15541 tcaaaaaaaa aggttgacct tgagatcaac cttattaatt ataagcctct aaaattattt
    15601 tgtgacaatt actaaatatt ttgaattact ccagaacaaa tcaggattag ttattttcaa
    15661 agattgatat tttttcttgt ctttttcata agtataagct gatgaaggat gtgaagacat
    15721 tatcttaaca ctctttgtat ttaattctac agaagcaaac tgtctgatgt caacttttgt
    15781 gaatgctgaa ttatcaatat cagggcttat cgtttttgtt ttaccgatac cgataaaccc
    15841 acctttctta ttcagtattt ttttgtcgcg taattgttta taagttccta ctgcataata
    15901 tgcagtgtta atctgattga ctttttcttc aatagtttta tcacgctcac cgatggttga
    15961 gtttaatgtt tcgatattag tgcgtaagcc ggtaatttca attttagcat caccaagttg
    16021 attgtttaag tttgcgatca tagaatcacg ttcggcaatt ttcagattca gtgaagtaat
    16081 cattttatta agctgagcac tttttacacc actgtttttt agttttcttg taagttcggc
    16141 aatacgctga ctgttctgat cgagcagtga agctatactc tccatattct tacggatacg
    16201 gtctttgaca gtgccgccca tttcagggtc gcgtttgcga tcaagtgtaa tagcagcatg
    16261 aatctgattg atgctgtcta aacttgcttc tattccgttg aagtcgccaa ggaatgaatc
    16321 aattgtgctg tctttaagaa aagatgtgct aatcaaagag tcacgttcac gatttagtct
    16381 atctacttcg ggcttgtagt ctttacaagc atacattgcc atcaatagaa atacgacaga
    16441 taacttaagg aaatttttca tttaatgaat ttttagtggt tctaatagtt agttattcct
    16501 gaaagagtat aataattgca aatatagatg catcaaatca tctaagcata ggctaacaaa
    16561 agggatttaa acaaagatat tcacacttaa caggttctga cagtacaggt aaggccaaca
    16621 gaaacaattt ctatctgctc ctgtctttct atccaaccgg tttcagcaac agcatcaaac
    16681 accctaccat caaccataat ttttccggaa ggtctgagag cagtatatgc ggttccgcgc
    16741 atgccgggtt ggatagcgtc aatatctgta tgtgctctga acccatgctc agagggttgt
    16801 gtagtttgga gtgtcaattt gcttagacgt tttgagtgat ttattttccc aataaaataa
    16861 aatatcaata acacagcacc aaacatggca ccaagactga ttaaaatgct ttgtgttatt
    16921 tgttccgaac ttgtgaaagt aaaatcccat cctttgttat tgatcatact taaagcaaag
    16981 cccattgtag tgcaaattat tcctgcaatg cctgcaacac caaaacccgg aatgacaaaa
    17041 aattcaagag ccaataaaat tactccactt acaaatagca atatctccca attggcagca
    17101 accccttcga gatataatgg agcaaaatat aagattgctg caacaaatga tgctgcaatt
    17161 ggaaaaattg ttcccggggc ttgaaactca aaatacaaac cggcaagcat aatcagaatc
    17221 aacaaactgc ttaatgcagg atgaattaaa aaactaatca tgttgtcaac ccatgaaggt
    17281 tgaaattcaa taattttatc gttattcaga ttttctaact ttaaaatatc agcttctgtt
    17341 tcaacaatgg cattacaata tttatttgtc atggcttctt ctgctgtaaa tgtcagcact
    17401 ctgccgctgt cattcactcc gggaataaca actcttgggt caaccatagc ttcagcaata
    17461 cgcgggtcgc gatttctttt ctctgccgtt gcgcgcataa tgccacgcat ataggattga
    17521 tatttatcag gtgctgcttc gccttgctct gttacaactg tagctgcgcc aatacttgaa
    17581 ccactgcgca tataaattct gttgcatgct attgagataa gtgctcctgc acttgctgca
    17641 tttgggtcaa taaatactac aacaggaatt tttgaattca gtattgcaat tctgatacta
    17701 tcggcagcat caagcatacc tccataggtg ttgaggtgaa ttaaaaataa gtctgctttt
    17761 tgctcatgtg cttcgcgtaa tgccttacta aaaatgcgtg ccatacctct atccacttcc
    17821 gactgaaaat taaacctgta aacaatgccg gcacaaacat ttgtaaccat aaaaaacaat
    17881 aacactaaag ctaaaaacaa ttttctcatt tcatttctgt tgaatattca aaattgcaca
    17941 attacaactt acatctgtcg ttttgatgtt aattaatttg ttcaaaacca ataaatgcat
    18001 cttcatttac caagcagtga tttttttagt attgtaaaaa taaaagtgat cgggatgaaa
    18061 atcaaagttt ctttattatt agcgacagca gcactcagcc tttcggctgc cgttgcacag
    18121 gaaggtttca gaactattgc aggcaaaaaa tgtcagcttt ataaagttgt atctaaagat
    18181 acatggtcgg gattatccag aaaatatcat aaagaaattg atgagttaaa agctgttaac
    18241 ccgggaatta acgaccttaa aataggtcaa attattaata ttccaattga aaacactgtt
    18301 aatgacaaaa gcgaaaacaa gtctgatact tctgctgata taaaaacccg caagcctgtt
    18361 gctacaataa acactccggc aaaaagcaac aacaagatac atgttattga aaaaggcgaa
    18421 acactttttg gcattgcaag aaaatatgat gtaagtgcaa atgatatacg caaatggaat
    18481 catcttgaaa atgatggaat aaaagccggc cagaaattaa ttgtttcaac caatgagaat
    18541 attactgtaa aaacagaaat taaaaagcag gagactacaa acgaaactat tctgcaacct
    18601 cttgacgaca aaaagttaga agcaaaaaac agtgaagtaa aaaacgactc caagttaatt
    18661 gcagaagcaa gtaataaatt aatccctcca tcaccttcgc ccagtgagct ggagtataaa
    18721 ccattgcgca ccgaagttaa gcgtagtggt ggtaagacag gtgcagttat tgaagttaat
    18781 gaaacaggta tggcctcatg gatcagagat ggtgatatca atcaaagtaa attttatgct
    18841 ttacatcgca atgctcctcc cggcaccata attaaagtca ccaacagaat gaatggcgat
    18901 tatgtttttg tgaaagtagt tggcacctta cccgacactg gcgacaatga caagcaaatt
    18961 cttaaaatgt cggaagccgc tgcaaaaaga attggcgcaa taaatgaaaa atttcaggtt
    19021 gagctaaatt acggcacaac caattaatag aaattgttgt tttataatac attgacgtac
    19081 gcactgccag gcaaaaagta tattggttct cctctatacc ggaaccaaca gtaaaccctg
    19141 tcggatttta caagttactt ttcttatttt tgcggtttat aaataaaatt ctgcataatg
    19201 aaagaagtat atattgtatc ggcagtgcgt acaccaatgg gaagctttgg tggtaaactg
    19261 tcatcatttt ctgctacccg tttgggtgct atggccatta aaggtgctat tgatcgcatc
    19321 aatctaaacc cttcagaaat caaagaagtg tatatgggta gtgttttaca agccaattta
    19381 ggacaggctc ctgcgcgtca ggcggctaag tttgccggcc tgccggacac tgttcaatgt
    19441 accaccatca ataaagtttg tgcttcggga atgaaagctg ttatgcttgc tgcacaaagc
    19501 atcatgcttg gcgacaatga tgtaattgtt gcaggaggta tggaaagcat gagtaatgtt
    19561 cctttctata tgcaaaatat gcgatgggga aataaatatg gtgatgtttc tgcaattgat
    19621 ggccttgcca aagatggact gacagatgtt tatcataatt atgctatggg aaatgcagca
    19681 gatttatgtg caagagaatg taatttttca cgtcaggatc aggacagcta tgctatacaa
    19741 tcttacaaac gcagtgccga tgcctgggct gcaggaaaat ttaaagatga aattgttcct
    19801 gttgaaattc cacagcgcaa aggggaaccg attctttttg cagaagacga agaatataaa
    19861 aacgtacatt ttgataaaat tcccggatta aagcctgctt tcagcaaaga aggaacagta
    19921 actgctgcca atgcttcaac gatgaatgat ggtgcagctg cattaatatt aatgagtaaa
    19981 gaaaaagcag agtcattagg actaaaacct cttggacgta tccgcagtta tgccgatgct
    20041 gagcaagcgc ccgaatggtt taccactact ccatcaaagg cattgccaag agcagtagaa
    20101 aaagcaggtt taaaaatgac agaccttgag tttgtagaac tcaacgaagc attttctgtg
    20161 gttgccttag ccaacatgaa agaaatgaaa cttgaccctg caaaagttaa tgtcaatggt
    20221 ggcgcagtag ctttaggtca tccattagga tgttccggtg caagaatatt ggttactttg
    20281 ttaaatgttt taaaacaaaa caatgcaaaa attggcggtg ctgcaatatg caatggtggt
    20341 ggcggtgcca gcgctatggt tattgaaaga atgtaactgc tttttaacgt aataatcaag
    20401 cggctgttcc tgtttcggag cagccgcttt gtctttaaaa ccaactgaac caaacaaaaa
    20461 aatgaatact aaattctaaa atcaaactat cttataatgg caatttgttc tttgctctga
    20521 accccatcaa ttgtaatact aacaaaatac aatccaacag gaatttctga cgtattgagc
    20581 ctaaccaatc cgacatcatt ttcaacggcc acaaaaactt catttcctaa aggattaaac
    20641 atttgtattc ttggcgtttc atcagtatca aactgaatag tcaataaatc ctttacaggg
    20701 tttggaaata tcgaataatg aaaaggcttt acataaactc tttgtgcagg aaatgtttct
    20761 gatgtaccat cataatctgt ttgtgacaat ctgtaccatg atgctccact aatcgggtag
    20821 ctatcagtat aactgtaatc atgtataaca ttactatttc cctgtccatt cacttctgcc
    20881 acttttgacc aattgtttcc attaacagat ttttctatgg taaaatgact gttatttttt
    20941 tctgaagcag ttacccagtt gagtttaact ttattttttt ctgcttttgc agtaaacgac
    21001 aataattcaa caggtaatgc acccgggtcg ttaaaaccaa atccaaaacc atgggctccg
    21061 ccaactgcac cgcctccggt catgtttgta gagcaatttg taaagaagga tgggcagcaa
    21121 ccttgttgaa tccaaccgga aaaacctgca ccaaaaccat cagaggtaat attaactgtt
    21181 gcatttgtat attgagggag gtttgaaagt ggtgttaagg taaaacaaaa ctcataagct
    21241 aaacaagaat tgggtgtccc ggtaaaatca cctctgccaa tacctgccgc atctataaac
    21301 tgtaatgtcc aggtctgccc accgtttgat acagttgcta cccaattgcc tgcattataa
    21361 aatccaggaa ttattggtgc agtctgcgga ttcagatatc ctgatgcagg aaagtcaaac
    21421 acaaatcttt ttacgccttc aacgccagta gtatttttaa aaaaagtagt tatacataca
    21481 gtgtccacaa catttagtgg cgagaaaaat aaaggtgccg tatcataaac tccattaact
    21541 gcaatctgaa aaaatggttt tgttggctca aagggaggtg ttgcacacac acttccatca
    21601 aatgaaagat tgccctgagt attgggaata ttaataactg taaaattcgt atcactgcca
    21661 aaagctatat cataagtaat tgttgctgca gcatcaaccc aaacataaag tactgtatca
    21721 tttgcagtat taacactaaa attacacatc ggttgcgtag tataaatact tcccagtggc
    21781 atacacgacc cacaaacttt ctctgcaaaa accctgaatg gccctgtaca gttttgtatt
    21841 gaaacaataa gtttacttgc tccatttgca atgtttacct gataaagcac atcttcacca
    21901 acaatgttac atgatggtcc tgtaaggtca tcgcccatgc cagcattagt ttgattataa
    21961 agtgttacac taggacaaat gttaacctgc aaaggattgc tacagttgtt ctgtgctgat
    22021 acctgcaatg taacaatcaa gcaggtaatg ataaaggtgg ttaaactctt catttggttg
    22081 gctattaatt tggagttata cttattgcag acagcatcag cttgctttaa cgcacttctg
    22141 ataaatgtct aaaccaaccg tttcaaagat tataccaaat caaaattgaa atttttcaac
    22201 aagtattctg tttcttaata aagtactgta aaggaaattc ggataatttg agaattgtga
    22261 tcaggaatat tggttctgat tataaagata taatcgagca ctgatgaata gaaagctatt
    22321 aaatttaata ttcttcaaca aaagtaaaca cattagtatt tattttgcag acaacataca
    22381 acaacaacat tttttttgaa agataatatg aataaaaata tcattcaaga tattagaata
    22441 gaactaaaga acatttcaag tgaagaagtg cgcattattg catcgcggtt ttttaaacca
    22501 ggcgaaatgg ccaaagtata tggtgtaaaa tcaccaatag tacggacaat tggtaaaaaa
    22561 tatttgcaac aagtaaagat attaactaaa aaggaaatat ttgcaatctg cgaagaatta
    22621 tggaattcat tttatcttga agagtcagtc attgcctgca tgtttacaga atcaatacat
    22681 aaaaagtttg aagctggtga tataaaaact tttgaaagtt ggataaaaaa ttatgtaaac
    22741 aactgggcat catgtgatac tttctgcaat cacacagtag gagactatat gatgatgtat
    22801 cctgaaaaaa ccgaattatt gaagaagtgg gctgtatcat caaaccgctg gttaagacgt
    22861 gccgctgctg tatctttgat agttcctgca aaaaaaggat tgttctttcc gttaatcatt
    22921 gacattgcaa ctattttact ggctgatgat gatgacttag tacagaaagg ttatggttgg
    22981 atgctgaagt ctgcatgtca gcataacgaa aaagatgtat ttgactttgt tatgcttcat
    23041 aaatccgaaa tgccaagaac tgccttgcgg tatgcaatag aaaaaatgcc tgaacggctt
    23101 aaatcgcaag ctatgaaaaa ataacattac tcctaccctg cacaaacaga acttcaacag
    23161 cactatgttt tattcaaaag gattaatcta ttttcgtgat gtttatttgc ttcactccta
    23221 attaatgaaa tttactttgc gacttttctt attgctttgt ttcacagtta ctcgccttgc
    23281 ggcacaggat gaaaatatca ataccgaccg tcctgatcag agtgacggtg tctatacatt
    23341 gaccaaaaag tattttcagg ttgaagaagg tataacttta ggcaaaaact tatttacaaa
    23401 caaccttatg attcggtatg gacttacaac ttctacagaa attcgtctgc tttcagatgc
    23461 aggaaagatt gatgactgga aaggttttct tccttttaca ttaagtgtta aacaaaaaat
    23521 tattaaacaa aaaaaatgga ttcccgccat tactgcagtt ggctatatga gttatggaaa
    23581 aattgcatcg aaagattttc tgactaataa aatttcttat gaactaaaat tagcgtttga
    23641 aaatgaagtg aatgaaaatt ttacagtttc atacaatgcc ggatttactg atgattttaa
    23701 aacatttaat tatacattta accttggtta ctcaccgata gaaaaaatat cactctttgc
    23761 tgagtatttt gcttctgtaa attcttctgt tcaacataat gccgatgcag gtgttttagt
    23821 tttgttgcat cctaaacttc aggttgatat agcttgcggt cattcaattg ccgacagtga
    23881 taatcgattt tacactacct ttggcatatc atataaattt taattccatc atgaagcatt
    23941 ttaagtcttt catttttatt cttttgacat tttgcacatc aaatcttttg gctcaggaca
    24001 aatacatacc tgttagtcaa actccggagc aaattaagac ctacattaaa aaccattttc
    24061 ctgctacgac aatttcttca gttaaaaaag aaaaagaatt acttaactat gagttggaag
    24121 taaagctaaa tgacagcacc aaacttacct tcaataagga caatgcatta atagagattg
    24181 aatcaaaaaa ggctatccct gaaaaaattc tgacagacac aatttatacc attgtgaaag
    24241 agaattaccc aaacaacaaa attgttggat gggagaaaac aaaacttggt cagaaagtgg
    24301 aattagataa tgatgttgag ctctatttcg atgacaacgg aaattttatc aggacagaac
    24361 attaattccg ggattaacga ctgcatttta gttgtaaacc aattattata aacacaaagt
    24421 cagtttctta gagggaaatt attcacagtc catacatctg ttgttcataa gattgccttt
    24481 cctgcatagc tgttttagct aatttcgcac agcaattaca gaaacagatg ccaaaggata
    24541 attccataaa atcagtactc atcataggca gtgggcctat catcattggt caggcctgcg
    24601 agtttgacta cagcggatca caagcttcac gttcactgcg cgaagaagga atagaagtga
    24661 cactcatcaa ctcgaatcct gccaccatca tgaccgatca ggtaacggca gatcatattt
    24721 atctgcttcc gcttacggtt gacagcattg aaaaaatatt gaatgaacga aagattgatg
    24781 ccgttttacc aactatggga ggccagacag ctttgaatct ttgcaaagaa gccgatgaaa
    24841 gaggtatttg gagcaaatat ggtgttcgca ttattggtgt caatgtagct gctattgaaa
    24901 ctacggaaaa tcgtgaatcg ttccgtaaac taatgataga aatcggagta ggtgttccgc
    24961 cctctgctgt tgccaactca tttcttgaag gcaaagaaat tgcacaacgc ataggatttc
    25021 ctttagtaat ccgcccgtcc tataccttag gtggaacagg tggaagtttt gtaatgaaga
    25081 aagaagagtt tgaagacagg ttgatgcgcg gcttacaagc ttcacccatt cacgaagtgc
    25141 ttgtagaaaa agctgttctc ggatggaaag aatatgaatt agaactattg cgtgatgcta
    25201 acgataacgt aataattatc tgctccattg aaaaccttga cccaatgggt gttcacacag
    25261 gcgacagtat tactattgct ccggcaatga ccttgagcga tcatacctat cagcgaatgc
    25321 gcgatatggc aattaagatg atgcgtgcta tcggaaaatt tgcaggcgga tgtaatgtac
    25381 agtttgccgt aagccctgac accgaagata tcattgcagt agaaataaat ccacgtgttt
    25441 cacgctcatc ggctttagct tcaaaagcaa caggctatcc catagctaaa attgctgcaa
    25501 agcttgccat tggttatcat cttgacgaat taaaaaatca gattacagga agcacttctg
    25561 cattttttga gcctgcctta gactatgtta ttgtaaaaat accccgatgg aattttgata
    25621 aattccatgg tgctgaccgc acattgggct tacaaatgaa atcagtcggt gaagtgatgg
    25681 gtattggtcg cagctttaac gaagcactgc agaaagcatg tcagagcctt gaaataaatc
    25741 gccatggttt aggtgcagac ggaacaggat ggcacaaagt tgatgacatc atttactcac
    25801 tagagcatcc atcatgggat agaatatttc atgttaaaga tgcattgaaa ttaggacttc
    25861 caatccaaac cattcacaac cacaccaaaa ttgataaatg gtttctgaca gagattcaga
    25921 aattagtagg catggagcaa gaactgagaa ggcatagtct gaaaaatctt tctacggact
    25981 ttatgctcga attaaagcag aacggatttt cagattatca gattgcacat attttaggta
    26041 atgtcactgc tgaagaagtt tatgaaacac gaaaagagat gggtgtaaaa cgtatttaca
    26101 aaatggttga cacctgctcg gcagagtttg ccgcaaagac accctatttc tacagtactt
    26161 ttgaaagacc tgcacaagac agtaatggta atgaaattac agccagtgaa agtattgtca
    26221 gcaataagaa aaaaatttta gtgttaggca gcggccctaa tcgtatcggt cagggtattg
    26281 aattcgatta ttcatgtgtt catggcgttc ttgcatcaaa agagtcaggc tatgaagcca
    26341 tcatggttaa ctgcaatcct gaaactgtaa gtacagactt tgacattgca gataaactgt
    26401 attttgaacc ggtttatcag gagcatatat tcgaacttat tgaatacgaa aaacctgaag
    26461 gcgttattgt tcaacttggc ggacaaacgg ctctgaaatt gtcgcagtac ctcaatgaaa
    26521 aaggaatcaa aataattggc acctcatacg aagatatgga tctggcagaa gatcgcggac
    26581 gtttctctga cttgcttcac gatttaggca taccatatcc tccctacggt gttgcagaaa
    26641 gtgcagaaga agcacttgtt gttgccaata aagttggata tcctgtcttg gttcgtccaa
    26701 gttatgtgtt aggtggacaa ggtatgagca ttgtaattaa tgacgaagaa ttagaacgtg
    26761 cagtaataaa actgttgaaa aaccttccgg gcaacagagt cctcattgac cattttcttg
    26821 atcgtgccga agagtgcgaa gctgatttga tttgtgatgg caaagatgtt cagatcatag
    26881 gtatcatgga gcacattgaa ccggcaggta ttcatagtgg tgattcttat gcagtgttac
    26941 cgccattcag cttaagtgat gtggttataa gtcagattaa agattatgcg aataaacttg
    27001 cattggcact taatgtaaaa ggcttattga atattcagta tgctgtcaag aatgaaaaag
    27061 tttatgttat tgaagccaat ccgcgtgcat caagaacagt tccgtttatt gccaaagcac
    27121 atcaggtgcc atatattaac attgcaacca aggtgatgct tggtgtaaaa aaattaaaag
    27181 acttcaacat taaacctgct caatttggat atgcaataaa agaacctgtg ttttcgtttg
    27241 agaaatttgc tgatgtaaat aaagagttag gtcctgaaat gaaatcaaca ggtgaagcca
    27301 taagatttat tgaaagcaca aatgatgcct atttccgtaa tttgtataag gaaaagagta
    27361 tgtatttatc aaaataattt ttacaatagc agagcatgcg tgatgtgttt tatacaatag
    27421 ttgttgtctg ggtggtaatt cagatttata aaagttttgt gaggcctaaa agcaatcaaa
    27481 cacaaaggaa cactaccaga caggaaggcg atgtgactat aaacacaaac accaataaca
    27541 acaatcgcaa caacgaccaa ggcgagtatg ttgattttga agaaatcaaa gattgatttt
    27601 actcgtagcg cagcgccttc acaggactta tgcgcgaaac aatcattgaa ggaataagca
    27661 tcaccagaat acatacaact aacgtccctg cattaatcag cagaatatta gtaaccgata
    27721 aatttacagg aacataagga acataatatg attcctgtgg cagactaacc aaatgaaaac
    27781 gatactgcat ccagcataat aaaagtcctg caacatttcc tgcaatcaaa cctatgccaa
    27841 caattcctga agaaaccatc agaaaaattt ttcttatccc tgaatttcct gcaccaattg
    27901 ccttgagtaa accaattgta gttgtattgt ctaataaaat tatcagcaac gtagaaacca
    27961 tattaatccc ggcaacaaga atcatcaaaa caagaatgat aattacattg acatcctgca
    28021 attcgagcca atgaaatatt tcaggataaa ttgttttgat ggattgtgta tttaattcaa
    28081 atcctgcttc tttatagata ctttcattga cggattcaag gtcgctaata tttttcagat
    28141 tcacttcaaa gcctcctgcc tgcaacacat tccagttgtt taatttctga ataacagtta
    28201 aaggacaaaa cacatagagg ttatcaaact cttcaagccc tgtggaatat attccattga
    28261 ctttaaactt gcgtgctctt ggcggttcat caataaaata aactacaaaa taatcattca
    28321 gctttaattt cattttgtct gctatacttt ttgaaataat agcaggataa tcacctgaat
    28381 gtatatgaag attaaaactc ccttcaataa tattcgggtc aaaaaaagcg gagttaaaag
    28441 ttgaatctac tccttttaca gccacaccta aaatatcttc agaagtgcgg attatccctg
    28501 ccttggttgc atatgcattg acactactga ttcttgtatc acttttaagc ttggctaaaa
    28561 atttctgatc aatatttacc ggaatctcct cataagattg attgttgtca aaatgtttta
    28621 ttctgatatg tgctccaaag ccaacaactt tagaagtaat ttccttttta aaccctgtga
    28681 caattgcaac agcaacaagc atcaccatta cactcagtgc aatgctggtt actgctatgg
    28741 ccagaacagt gcccgacatt cgttggttgt taacactgcc tgtgaaaaaa cgttttgcta
    28801 tgaagtaaga gtatctcaac taatttatta ttcttgctga tatttcggtt tcaaatttat
    28861 gattttgaag cgttggatag ttttactatc cctaattatc agccatcaaa gcactgcgca
    28921 aatgattgac agtgtaatta ccggtgccgg acaagttaac agctatttgc cgatcatcag
    28981 tgataaaaat attgctgtgg ttgctaatca gacaagcatc gttggcaaaa cacatttggt
    29041 tgacctgctt ctttcaaagc atgtcaaaat aaagaaaatt ttcactcctg aacatggttt
    29101 tcgaggcact gccgaagccg gagaaaatat tgaatccggt aaagataaaa gcacaggcct
    29161 acccatcatt tcgctttatg gtaatcataa aaaacctaca actgatgatt taaagggtat
    29221 tgatattgtc ctgttcgact tgcaggatgt cggtacacgt ttttacacct acagttcaac
    29281 attacaatat gtgatggaag cctgtgcaga aaacaaaaag aaactgattg ttttagacag
    29341 acctaaccca aatgggtatt atgttgatgg ccctgtgctt gaacctgaat ttaaatcttt
    29401 tgtcggcctt aatcctattc ctgtagtaca tggcctaacg atgggcgaat atgcacaaat
    29461 gcttaatggc gaagggtggc ttgccaataa agagcaatgt gatgttaccg ttatcagcgt
    29521 taaaggatat acgcatgaaa caagatataa actgccggtt aatccttctc ccaatttgcc
    29581 aaacatgaca gccgtttatt tgtatccttc attgtgtttt tttgaaggaa caattgtgag
    29641 cattggtcgc gggacggcct atccttttca ggttgttggt taccccggtt ttactgaagg
    29701 cccatttgaa tttactccta aaaaaaatcc ggagatgaaa cttcccccac tatatgaaaa
    29761 ccaaaagtgc aaaggctttg acctgcggga ttttggagat tattatattc catcacttaa
    29821 aaaactatac ttgttttgga ttaaaaatat ttacgacaac tatcagcaaa aagataaatt
    29881 ctttaacgac tacttcgaca aacttgccgg cacagataaa ttacgtaaac agattattgc
    29941 agggctgaac gaagaacaga ttagacgaac atgggaagaa ggtttgaagt cgtataaagt
    30001 tattcgaaaa aaatatctac tctatcccga ttttgaatag ctattacaat ttagtttttt
    30061 caaatacaca aatggcttta tatgtcatgg ttttaaccct cgtcaattct tttgtatttt
    30121 tgcactttat caaccgtatt ttttgaaaat atataaccat atagaccaat ttgaaaaggt
    30181 cagcaatgcc gtaataacac ttggcacatt tgatggcgtg catctagggc ataaagtaat
    30241 tattgaacga ctgaaaaaaa ttgccggtga tgtaaacggg caagttgtag tactcacatt
    30301 ttttccacac ccccgaatgg ttttatttcc tgacgaccat ggaattcaac tgctgaatac
    30361 aataaacgag cgaacgatgt tgatggaaaa agcaggcata gatcatctta ttattcatcc
    30421 cttttcaaaa gaattttcaa gaatgagttc taccgaattt gttcgagata tattagtcaa
    30481 caaacttggt gtaaaaaaac tgataatcgg ctatgaccat cattttggac gtaacagaga
    30541 aggttctttt accgatttgg aagagttggc accggtttac aattttactg ttgaaagaat
    30601 tcatgagcaa ttagtaaatg atgttgccgt aagttcaaca aaaatcagaa actctttgct
    30661 ggcaggcgat gtggcaactg cccgtgagtt tttgggatat gattatgagt tgactggtac
    30721 tgttgttcat ggcaaaaaat taggtcacag tcttgggttt ccaacggcca acctgcacat
    30781 acatgaggat tacaaattag ttccggcaaa tggagtttac gcagcccgaa ccctggttca
    30841 tggtcaatgg tatgatggcg tgatgagtat tggaaacaga cctactttcg acaatggagc
    30901 acgctctata gaggtgaatt tatttgattt taatgctgat atttacggag aaaagataac
    30961 catttgtatg actgaattta tacgcagtga gcagaaattt gaaaacagtg caatgctgat
    31021 tgaagccatg aatgctgata aattggctgc aattaaactt ttatcgtaaa atttacgtta
    31081 aacgagtcat gaaacgcaac atcatcattc ttctgatttt gctatttgcc tcccctgctc
    31141 atgctcagga gctatgggac gatgctgaaa ttgatacatt gcctgtattt cgttcactga
    31201 atgcagcaat gcaaaacccg gattcagtat tcagacttga cctatcaaaa caaaagttga
    31261 aagagatacc gccacaggtt tttgagctta cacagttgcg ggagcttaat gtcagcagaa
    31321 atcatttgac tgaattacct gaacaaattt acaagctcaa taacctgcaa cgtctgtatg
    31381 caaacaacaa caaattgact gcattgccgc aacggatagg tacgcttaaa aatcttcaga
    31441 ctttaacact cgatcgcaat cgtattgagg tattgccacc tgaaatgggc atgttacata
    31501 atcttgaagt tttaagtctt tgggataatg agttgtctga aatacctgat gaaataaaaa
    31561 gcctttcaag tgtattgaaa aaattagaat taagaggaat tcttttcaat gaagagcaac
    31621 aaaaaagaat ccacgactta ttgccttata caaccatcta tttttcgcct gcctgcaact
    31681 gcaagcaata atccaagtca gtctgatttt atacttttgc ctgtatgtta cagacagaca
    31741 aactgcattt acgtgcctgc gaacctgatg acctgccatt gatgtatcag tgggaaaaca
    31801 acgctgatta ctggctgctt agtgacacaa caaaaccgtt ttcaaattac accattgctg
    31861 agtttattaa acaagatcag aatgatattt ttacaaccaa gcagcaacgc tttgtaattg
    31921 aattacgaaa tcctgtcaag ccaattggat ttattgacct ctaccaattt gaacccgttc
    31981 acaggcgtgc aggtgtaggc attctgattg ctgacctaag cgagcaaaaa aagggctatg
    32041 ctgcgcaaag tcttgaatgt ttaaaaaact attcacaaac agttttaaat atgcatcagc
    32101 tttactgcta cattcaggca tcaaataaaa agtcattatc actctttcaa aaagcaggat
    32161 ttcataaatg tggaatattg aaagattggc tcatcaacaa caagacaccc gaagatgtat
    32221 tgatgttcca aattatattc tgattacttt ttattaacaa ttgttagtca cactcaggca
    32281 tattgaacct ttttgttcag ttttgctaca cttctaaatc aaacaaaatg caaacattat
    32341 ctgcaaccat cgataaggta aaagaatttc atgcaacctt cggccttcaa tattttgaaa
    32401 atccacaagc tgcaatagat aaaaatatta tctcgctccg tcataagcta atgcaggaag
    32461 aaaatgatga atatctggaa gccgcaatga acaatgatct tgagcaaatt gctgatgctt
    32521 tgggagataa actatacatt ctatgcggca ccatccttgc tcatggattg cagcataaaa
    32581 tcacagaggt atttaatgaa atacatcgca gtaatatgag taagcttgac gaaaacggaa
    32641 aaccaatcta tagagaagat ggtaaaataa tgaaaggcaa aaattatttt cttccggata
    32701 ttaaaacagt attggagcag tgattcaaaa gctgagtatt ataattccgg tctttaatga
    32761 atctgacacc ataactactg tattggaaaa agttaatact gtacaactga taaacaacat
    32821 ttccaaacaa attattgttg ttgatgacca ttcaagtgac aatagtttag aagctataac
    32881 acaatttaaa aataagcatc cacatactga aattaatatt gttcagcaac aagttaatat
    32941 gggtaaagga tatgctgtga gaatgggcat caatgtatct actggcgatt acattatcat
    33001 tcaagatgca gaccttgaac tcaatcctga agattttaat cttctgctaa aaacctctat
    33061 tgaacgaaat gtacaagtag tctatggctc tcgattttta agaaaacaaa aatttaaagt
    33121 gccatttatg actttaatgg caaatagaat attatcatta gtaacaagtc tgctgtcagg
    33181 caagaaaatt actgatatgg aaacatgtta taaactaatt gaacgttcat tactacataa
    33241 cattcaacta aaagaaaatc gttttggttt tgagccggag attactataa agctcctaaa
    33301 aaacaagcgt gttcgttttg cagaagtgcc tatttcctat caaccacgca caaaaaaaat
    33361 gggaaaaaaa ataggagtta aggatggaat cagggcatta tactgtttat ttcgctaccg
    33421 gattttatga aaaggagcat tatctttatt gcactttttg catttttaac actcattgct
    33481 ttcgtgtggc agcaacgcac ttttggcaat gaaaaatacg aacgcattgt tgatagtgat
    33541 ggtaaaggct atgttgccta cttgcccgct atatttattt atcaggactt aagttttaat
    33601 ttttattcca aaccacaaaa tcagaaaata gccagatatt tcaatgatag gtttctcaca
    33661 aaacaaaatg acaaaactat tttaaaaacc tatgttggag tagctgtttt aatgacacct
    33721 ttctacctga ccggatgttt gatgcaaatt gtaattcatg gcacatgtaa cggatatgag
    33781 cctttagttc aattttttat ttctattgac gctatttttt atgctctggc aggactcttt
    33841 ctgatttata agacactgcg tttattgaat tttagtaaaa ctatttgtgt gctaacttcc
    33901 gcattgatat ttactgctac aaatctttgc tactatagcc tcttccaccc taccatgtca
    33961 catgtgtatt cgtttttttt aatcgctgca tttgtttatc tatcaatctg cctcacacaa
    34021 aatacaagta ataaatattt actgttatct gccatcatat tagggttaat tgtactcgta
    34081 cgtccgctaa atattattat cattctcttt cctttgttgc tgttaggaaa acaaaaactg
    34141 actgatctgc taaaaataaa aaccagaacg cttttagtct ctgtattatt ctttttaatg
    34201 ataatttcac ttcaacccat attaaatttt ttgcaatgtg gattgctttt cccttggagt
    34261 tatggcgatg aaggttttta ttttactaac cctacactca taaaaacact tttcagtttc
    34321 agaaatggat tatttattta ttcaccaata ttactactta caataccggg attgtggttg
    34381 ctacgttaca attcaatcaa aatcacttct gtcatagcaa ttgtattgat tattatctgg
    34441 tatttacttt ctgcctggtg gaactggtca tcagagccag catttggcaa cagaagcttc
    34501 gttgattggt atggactaat gtcattacca gtggctgcat caattggcta tggatggaaa
    34561 aaaagtaaac tacttttatc attactctgt ttgggttttg cagggctaag cacattacaa
    34621 acctggcaat atctcaatga cataatacat cctgacggaa tgaattttga aaaatatcgt
    34681 tatgtttttt taaagacttc tccggaatat attgcctcag tagggaatgg aaatgaggaa
    34741 gcttatttac ctgtacaacc tctaccattt attacaacgg aaattaatcc ttacaacaaa
    34801 gtaacaacaa gcaatcatgc cgaaacaatt tttgaattta attcacagac atataaagga
    34861 gcacaatttt caattccttt ttcaaataat caacttttgc cgatgaaagc tatggtaacg
    34921 cttcaaagaa aagaaaacga gtttaaagcc tgcacacaag ctaccgtttg ttttgactac
    34981 accgattcaa ccgggaaagt ctattataat gaaaatttaa gaatcaatga tattcccaga
    35041 aaacaaatta atgcgtggaa agcttttacc tatcattttg tcatgccggc tttaaaagac
    35101 acaaattcac aattaaactt ttatatcaac aatcctaaag gggctctttt tgaaatcaag
    35161 aacatagatc taaaattatt caatttttca aacaagcaat agtaagccca tcaatccgtt
    35221 ctaattattc tatttttgca ttcgatgcaa cgaaagttta tcaccaacct tgccttacta
    35281 cttctgctga acctacttat caagccattc tggattttcg gcattgaccg taccgtacag
    35341 aatatgatag gtgcagaagg ctatggaaag tattatgcat tattaaactt ctccttcctg
    35401 ttaaatatca tccttgatgc aggaatcact aattacaaca acagaaatat ttcacagcat
    35461 cagcatttag tatccaagca tttttcagga attattattc ttcgtttttt attggcaggt
    35521 gcatacatac tcataagtct gattttagga ttaacgattg gttattcaaa agaagatatt
    35581 cacttattac taatactttt agccaatcag ttttttatat catttattct atatctccgg
    35641 tcaaaccttt caggattaca tcttttcaag caggatagcg ttatttcagt aacagatcgt
    35701 tttttaatga tactaatttg cggttatttg atttggtata acaacggggc gaatttaaac
    35761 attggaaatt ttgtatatgc tcaatcggca tcctatctga ttacggcaat agttgctttc
    35821 attctcttac aagggcgtat cagttttctg aaattaaact ggcaacccgc attctatatt
    35881 gccattctta aaaaatcact gccatttgca ttactcatcc tgctgatgac attctacagc
    35941 agaatagatt caataatgct cgaaagattg ttgcctgaag gggcctttta tgctggcgtt
    36001 tatgcacaag catacagaat tttagatgct gtaaatatgt ttggattttt atttgcctct
    36061 ttgcttctac ccatgttttc gcacatgata aaaaaaagac aggatattca gccattaaca
    36121 aaccttgcat ttaaacttct gatgattcct gcattgacat tggcagcatt aggactgttt
    36181 ttcagcaatg acattatgaa cttgctctac catgagcatg gcgatttgtc tgctcccgtt
    36241 ttctccattt tgatgtttag ttttgttttt atttcgacat catacatttt tggcacgttg
    36301 cttacagcta atggaagctt acgaaacctc aaccttactg catttggtgg aatgatattg
    36361 aacatttcgc ttaacttata tctgatacca caattcaaag cgtcaggtgc agctttagca
    36421 ggcttgatta ctcaaattat tatggcagtg gcacaaataa ttctggctgc aaaaatcctc
    36481 cattggaaac ctgactggaa gctcaacagt aagttattgc tgttttttat actaatcgct
    36541 ttaatcgcag atatagcaat tcgttatttt ccggaaatta agacaacact tgtcataatt
    36601 ttactggcac caatacttgc attttcgctg gggctgcttc gtatttcaga actaaaatca
    36661 ataatgaaag agcgtcaata gcgaaacgct aatttgttta catttgcggc tccttaataa
    36721 attccttcat aatgaataat acccctgttt acgaagaaaa cttcgcatca ctaagcctga
    36781 ttcaattgat taaaaaatgg aaaaagcaat tgataattgt agcagtaatt tccattgtac
    36841 tgtcatttat ttttaccctt ccgtttttta ttccaccgat gtttaagtca acggctattg
    36901 tttacccggt aaatcttagc ccctattcaa ctgaaagtgc tactgaacag ttattacagt
    36961 tgctcgaatc tgataaaatt aaaatggagt tgataaaaga ttttaaactt tatgcacatt
    37021 ataaaattga ctcaaccaag aaaacttcat taactacagt cttcaaacaa cttgcaggca
    37081 atttaagtat caataagacc gacttcgaat cagtgaagat tgaagttatg gataccgatc
    37141 catttcaggc aaaacaaatg gccgattcaa taatagaaaa aggcaacagg ttggcaagaa
    37201 acctctacag agaaaagaca atggaaggtt atattgttgc taaaacacaa tatgatatga
    37261 aaaaagccga gctcgactct gccgataaag ttttaacaga gttaaggcta aagtcaggta
    37321 tcattgagtt ttcacaacaa acacgcgaaa tttcaagagc ttattatact tcaatattaa
    37381 ataaaagcaa taatgtgcgc ttagaagata tgttccataa attgcaacaa tacggctcgg
    37441 attatgttac cctcaatgaa aaaataaact atacaagaag tgtttttaat aactacaagc
    37501 agtatgtaga aaatgcagaa aaagatctta ctaaagagct atcatactct aacgttgtta
    37561 cacccccaat gattccggat aaaaaagcct atccgctgcg aagtctgatc atgttgatgt
    37621 ttaccgcctc agttgttttt ttatcatttg tggtaatcat catctacgaa aattcacagg
    37681 aaaagaaaac acaaagcaat taattgaatg caggtcattc aacataaatc actccgttgg
    37741 ttttatggcc ttactgccat atatatgctt atggctatat tcagtttggt gaatgaattt
    37801 tattggctat tgattttgcc tgcaggtatt cttattggat attctgcact ttatcatctt
    37861 gacaagcttt ttctttttac agctttttgc acacccttag caattaactt atccaagact
    37921 gatttaggca taggtgtcag tctgccaaca gaacctattc tgtttggtat gatgctaata
    37981 tatctacttt caaaaagtat tgatggcaga tttgaaaagt cgtttatcaa acatccgatt
    38041 actattctca ttttgattca tcttgcatgg atgttgatca ccagcttcac aagcagcatg
    38101 tttcttgtat ctattaaaca ttttgttgca agactatggt ttgttgtaat tggctttatg
    38161 cttgccacct ctgtctttaa ggatgaaaag aaaatgaggc agtttatatg gttctatgct
    38221 gttggtatgg caataaccat aatttatacc atcacacgtc atagtataaa tggatttact
    38281 cagcaatctg ctcactgggt gatgacacct ttttataatg accatacaga atatgctgct
    38341 gtacttgctt tgatgctgcc tccaattttt ggattgatgc ttttgaaaca taaatctttt
    38401 atgtactggt tttttgcatt tctgatttca ggattggtaa ttgttgcaat cgtattgtca
    38461 tacacacgtg ccacctggct cagtctggct gtggcacttg tctctatgtt tgcttatgtt
    38521 ttcagagtaa aaacatgggc tgtagtagca ataataattt caatggttgt cggctttcag
    38581 atttttcagt caacagtgtt atacaaattc ggaaaaacaa aagagtattc acagaatgac
    38641 taccgtgggc atttaaagtc tattaccaat attaaaaccg atgcatcaaa tgtggaacgt
    38701 cttaatcgtt ggagttgtgc atggcgaatg ttccttgaca aaccaatctt tggatggggg
    38761 cccggcactt atcagtttca gtattctgtt tatcagttac agtttgagaa aacatacatt
    38821 agcaccaatc ttggcgataa aggaaatgca catagcgaat atcttggtcc gttaagtgaa
    38881 caaggtttta tgggaactat aatttttgta gcattagctg ttgtggttct ttacaaagca
    38941 tcacgcgtct ttttgcaatc gaattcaaac aacattaagg tgcttgcatt gtccatgcag
    39001 attggattaa tcacgtatct ggttcatgga tttttaaata atttcttaga taaggataaa
    39061 gcctcaattc cattttgggc attcgtggct gctattaccg ctatggatta ttatcacaat
    39121 aagaaacaac taaatccatc agatgagcga gcgcaatgat aagatggtaa catcatcact
    39181 gaaactattg ccttctctga actgatctaa gtccgcaatc aatctttcat gaaatttatc
    39241 aagacttgaa ttgtttatat tctgctctat aaataacttt aatgcctcca taccataaaa
    39301 atcaccttta aggttttcga gttctgtaat gccatcggtg taacaaaaaa cagaatctcc
    39361 actactaatc tttacatgac cttcatttat aaatggcaat tcatcaaaca tacctaaacc
    39421 tgtagtgcct ttatctaaaa gttcagactt gtttttacta cataaaacag gaggattgtg
    39481 accggcatta atataattta tttcaccttt cttaaaattt atcaatccga taaaaaaggt
    39541 tataaacttc tcaccttttg cagtcttact cacacagtca ttcaattcag caactaagtt
    39601 tgtcaacaca ggccggtttt ttattaatgc atgcaggtta gcctgaaaat tagacattag
    39661 caatgctgct gccattccct tgcctgaaac gtcagccatg caaaaaatat gttcgtgttt
    39721 gttgagtgaa ataaaatcat aataatcacc tcccacttgc tgatgtggaa gataatgtgt
    39781 tgctatctcg aaatattcat ttccgcataa gccaactgga aacaacattt cctgcatttc
    39841 tgctgccaac tcaagctctt tacgaaatcc tgcttgtgct actgcttctt tatttaaacg
    39901 tttgttttct attgctacca caattatgtt tgttatggta tgaataaatg gaacaacttc
    39961 ttttattgaa attgactgac tcgggtctat tgcgccaaca atacaataag caagtaacat
    40021 ctcattgtga taaaccggaa taaccgattc atctttctca agccactgtg gtgtggctac
    40081 tgtttttgtt ttagaaaatg atatttcatt gagaaacaat tcaggctgca agcggccgag
    40141 ctctgctttc atgccatagg acagcacctg cttccaacca ttttcattga caaacaaaga
    40201 aagttttcct gcttttaaat gatcacgcaa tacgttttca tatatttgat acaattgctc
    40261 cgcaggcaga ttgtaattaa ttgcttttgt gatctgcaaa agccaatgca actgtacttt
    40321 ttttgactga agctcggaag caagtttatc ggtggtttct tccatcacta ttttttgtta
    40381 tccagaaact caaacacttc aggcaatcgc ttcatcaatg caagctcacg catttttaaa
    40441 cgggcgtcag ttgtgggact gccgaagtaa gtcttacctg cttctaatga tttaccaacc
    40501 cctgactggc caagtaccac tgctcctttg ccaattgtca aatctttctg aacaccgact
    40561 tgtccccata atataacatc atcttcaata gtgacaacac ctgcaatacc cacctgtgca
    40621 gcaaacaagc agttctttcc aatcaccgta tcatgaccta catgaacatg attatccatt
    40681 cgtgttccac ttccaataac cgtatcgcct gacacaccct tatcaatggt acaggaagca
    40741 ccaatttcaa cagaatcacc aataattaca cgaccacagc tatgcatttt atcaaatcct
    40801 tcgggacgtc ttttaaaata aaaagcgtca gcaccaataa cactgttggc atgaatgata
    40861 acgccatgac ctatttcgca atgatcatag ataactacac ccggatatat caggcaattc
    40921 ctgcccacct tacaatgtgc accaacaacg ctaccctgca tcaacacagt gccttcgcca
    40981 accacagcag tttgatcaac ctgttgtaaa ctaaaagtta atggtctgta ttttcttgtc
    41041 agcagattat aatctctgaa cggatcgtct gaaatgatta atgccttgcc ttcaggacag
    41101 gaaacttctt tatttatcag aatagtagtt gcagcggaat taagggcttt atcataatat
    41161 ttaggatgat ctacaaatgt aatatcaccc ttttcaacct tatgtatttc atttatacct
    41221 gtaatctgaa agccgtcatc gccaatatat tttgcattca ggagtgaagc tacatctgca
    41281 agcctgattg ctggtactat tttcatattt caaactgttt gactgcaaat gtaacaaatg
    41341 tgttgtcaat tctcttctga attacctcaa tcaatttatt aaaaaaataa cagtgtttaa
    41401 caaaaatatt ttctcaggtc tgtaacaaat atgttttttt gcgcgtccta taatcaactt
    41461 caatcattta taccggatta atttttcagc accaaaatat atatgaaaaa attctttgtc
    41521 cttacttaca ccatattttg ttttacattt tcatttgcac aaaaacctac tcagacagtc
    41581 agaggtacaa taactgacaa gcaaagtaaa gagcctttaa ttggagcctc tattgtttgg
    41641 cttgatacat caatattcat tggcacaacg gctgatataa atggcaactt caggctaaat
    41701 aatattcccg ttggcagaca agcttttaaa atctcatttc ttggctatcg cgaaactgta
    41761 atcacaagca tcatcacctc aggtaaagaa gtggttttaa atattgaatt ggagcagtct
    41821 gtagttactg caaaggctgt tactattgtt gcagaaaaac aaaaagacaa acccaataat
    41881 gaacttacta cagtaagtgc aagatcattt acaatagaag aaacttctcg ctatgcagga
    41941 agcgttggcg acccatcgcg tatggcagca aattatgctg gcgtgagtgg cgcaaacgat
    42001 tcaagaaatg acatcatcat tcgaggcaat tcaccaactg gattgttatg gagactcaat
    42061 ggtatagaaa ttccaaatcc taatcatttt ggctcattag gcacaacggg aggaccaatt
    42121 tcaattctaa acaataatgt acttgacaaa tctgattttc ttaccggtgc ctttccggca
    42181 gaatacggaa acgttcttgc cggtgtattt gatttgcaaa tgcgaacagg caacaatgaa
    42241 aaaaatgagt ttatggcaca ggttggcttt aatggttttg aagccggtgc cgaaggaccc
    42301 atcagcaaaa aacaaggctc ttcttactta ataaattatc gttattctac actgggagtt
    42361 tttagtgcat tgggaataaa cttcggaaca ggaactgctg tgcctaaata tcaggatgtt
    42421 tcttttaata ttaatcttcc aaccaataaa gccggaaaat ttaccttgtt tggcatgggc
    42481 ggcatcagtg gtattgaagt gcttgaaaaa gacagagaca caactaaaaa aaatctttat
    42541 gttgatgata aaagacaaaa tggttacttc gataatacta tgggcgttgt cgggttgatg
    42601 catacttttt ttataaacaa ttctacctac accaaattca gcctttctgc atctattgca
    42661 gggcaaaaat ataaagtgga taccctgcca gactttgttg attatgctgt gccatattac
    42721 agaaataaat ctgatcaaca aaaatacagt gctgacttca gtcttaacaa aaagttttct
    42781 tcaaaaaatt tcttaaaaac cggcttcagc ttttctcagt atcactacac ctatcgcgac
    42841 agtggtgtag tggacagtgt ttgggaatct tatacggatt ataatggcaa tgccttactc
    42901 accaaactct atgtggaatg gcaacatcgt ttttccgatc aactttcact gaacagtggc
    42961 gtttacgcac aacttttcaa ttacaacaat acttatagta tagaaccccg cattggtgcc
    43021 aaatgggaaa tcagcgataa acaatcgcta agttttggaa caggattaca cagtcagtta
    43081 caacctatgt acatctattt tgtaaaaaca caaaaagatg atggaagcta ttacgaaacg
    43141 aacaaaaaac taaaaatgac aaagagctat catgcagtgt tggcttatga cagaaatatt
    43201 tcaaaggttt tcagaataaa aactgaggtg tattatcaat atctgtttaa tgctcccgtt
    43261 gaaaaacgac ctacattttt ctcagtgctg aatgaaggtg ccgactttgg tattgcagga
    43321 attgacagct tagaaaataa aggtaccggg tataactacg gttttgaatt tactgcagaa
    43381 aaattttata gtaaaggata ttacttcctc tttactgcca gcatctttga gtcgaaatac
    43441 aaaggctctg atggcattga acgaagcact gcattcaata gtaattttgt tttcaattta
    43501 ctgggtggaa aagaatggac tatcagaaac aaaaacacaa tttcaatcaa cattaaaaca
    43561 acctatgccg gtggacgcag atacattccc attgactttg ccgcttcaca gattgcagga
    43621 gaaacagaat acgatgaaga ccatgccttt gaacatcgca gaaaagatta tttccgcaca
    43681 gaccttaaaa ttggttaccg gataaacaga aaaagactga cacatgaaat atccatagat
    43741 ctgaataacg tctttaatac gcaaaacatc tggaatcagc agtatagtgc caaaacaaat
    43801 actgtaaaaa ctgaatatca gatgggcttt ttaccaattc cgatgtataa atgtacattt
    43861 taataaagtc aatcagaatg atagtgcatt gtcttccgaa gtttccatga ataatcttta
    43921 agctaactac tgtaattctt taaaattgca ctgccattga ctaactttgc cgcatggcag
    43981 atgtgataca attgttgtcc gatactgttg ccaatcagat tgcagcgggc gaagttattt
    44041 taagaccttc atcggttatt aaagaactga tggagaatgc tgttgatgca ggcgcaacac
    44101 gcattgatgt gagtataaac gatggcggaa aaaatctgat acggataacg gacaatggct
    44161 gtggcatgag cgagactgat gcacgtcttt gtttcgagcg ttatgcaaca tccaaactac
    44221 gcaaggcaga tgatttatat gccatcagaa caatgggctt cagaggagaa gcaatggcat
    44281 ctgtggctgc ggtctgtcag gtagaattaa agacgaaaag ggaagctgat gaaaccggta
    44341 cacgcataga aattgaaggc tcagagttta aacttcagga gccatgtgtg atgcagtcag
    44401 gaacaatcat taccgtaaaa aatattttct ttaatgttcc tgcaagaaga aattttctga
    44461 aagcaacccc tgtagaagcg cgtcatgtca tggatgagtt tctgagagta gctttgcccc
    44521 gacctgaaat tgggtttagc cttcaacata atggtatgga agtttacaac ctgcctgcaa
    44581 cacatcagcg tcagcgaata tgtcagattt ttggagctgc ttataatgaa agattggtac
    44641 ctgttgaaga ggacacaacc attttaaata ttaaaggatt tgtaggtaag cctgagtttg
    44701 caaagaaggt cagaggtgaa cagtattttt ttatcaacaa acgttttgtg aaagatgcct
    44761 acttgaatca tgccgtcaca attgctttca atgagttgtt gcctcagggt acttttgcat
    44821 cgtacttttt gttttttgaa gttgacccgt cacgtattga tgtcaacatt cacccaacta
    44881 aaacagaagt taaacttgaa gacgaaaaat cggtttacgc tatacttcgt tcttcagtga
    44941 aacgcgcatt aggccgattc agcattgcac caactttaga ctttaatcag gagcaggctt
    45001 ttaatgtacc gctctcacag ctcaacacaa ttcctgtaca accacaggtg aaagttaata
    45061 ctaactacaa tccatttaaa agtgtagaga taaataaacc tacccaacaa cagaatgggc
    45121 aatgggaaaa actttatgag attgcaggtt tgaccgatgt acaacagaca aaactaccaa
    45181 tagctcctgt tgaagaaaat agcgcatcgg catttatgca actttttaac agctatattg
    45241 cagtggccat gcaagatgaa ttatggctta ttgaccagca attagcacat gagaaaattg
    45301 tgtttgaaaa aacacttttg cagatgaaag gaacaccaac tgttatgcaa caaagtttgt
    45361 ttccgctaca gattgaactt gacaacagac aaaatgccac catttcatca ctacttgaag
    45421 acctcaacca ttcaggattt gatttacagc catttggtca gaatactttt gtgcttaacg
    45481 ctgttccctc ctatctggaa accggaactg aaaagaaaat tattgaaatg cttattgatg
    45541 agtttatcag tggcgaagct tctgcataca atccaatgga acgtgttgcc atagctattg
    45601 cgcgcagtgc ttgtttaaaa agaggtaagg ttctttccgc aagagagatg tctgttcttt
    45661 ttaatgattt gatgcaatgc gaaatgccct actttaccaa aagcggtaag gcaataattt
    45721 ttaaattaga aaaaaatgaa attgaccgaa agtttgagaa aggttaattc ctatttaaac
    45781 caaagaaaat atcatctgtt taaaatgatt aaatacggaa acctatcttc gcaccgttta
    45841 accattgatt accaaaatat gtaatcgttt tttatgcagg aatttcgacc ttcaagtttt
    45901 aaactattac ctgatgtaat taaaaatatt atgatcgtca gtgcattggc atactttgct
    45961 gatctggcgc tcaataaaat cgggtttgat cttaatgctt atgccggact ttacttgccg
    46021 gcctctgaaa attttcattg gtggcagtac ttttcttaca tgtttttgca tggtagcctt
    46081 acccatattt ttcttaacat gtttgccttc tggatgtttg gtggcatcct ggaaaatcta
    46141 tgggggccaa aacggtttct gatttttttc ttagtaacag gacttggtgc tgcattaata
    46201 catacagccg ttggatatta tgaattgcat tcgttacagg caaaggccga tgcgtttttt
    46261 aacgctccgg gtgccgatgc attcagtatt tttataaaag ataatatccc atatcaatat
    46321 ctcaacgctg attatgccat ggctgttgaa ggtattaaat caaactggta tgcacatggc
    46381 gacagcccaa tgattattga acaggcccgc caggcaattg atgaatacat tcgtttcaaa
    46441 accaatatac cgacagttgg tgcttccggt gccgtttatg gtgtgctcat ggcatttgga
    46501 attcttttcc caaacacctt aatctatctt tactttttta ttcccctaaa agcaaaatat
    46561 ttagtgctca ttattggcgg catagagttg tattctgcat ggcaaagcaa tccaacagac
    46621 aatgttgcac attttgcaca tcttgggggt atgcttttcg gatatttgtt acttcgttat
    46681 tggcagaaaa ataaccggtc acaacttttc tgaacatgct gcacaatgtg aaaagtaatt
    46741 acaaatcact cgatatgcta caaaagatta ttgtagttaa tgcaattatc tatttgtttg
    46801 taaatctggg tgatgttttg ctgcgacttt ttaattttcc ggatggcatt ataagcggct
    46861 ttttgactga ctggcttgct gttccttctt ctgtaaagtc attgctttat cgtccgtgga
    46921 cattaattac ttatagtttc taccacgaaa actttcttca cattctgttt aatatgttat
    46981 ggctgttttt tatgggtcgt ctttttacag aatatcttgg tgaaaagaaa ttattaacac
    47041 tttatcttgt tggcggctta tgtggctctg tattgtttat tgtattctat aatttgtttc
    47101 cactattcag tcgcgaagtt gacattgctg ttgccttagg tgcttctgca agtgttcttg
    47161 ccataactgt tgccattgcc acattattac cggattacga agtaggactt ttcctgccgc
    47221 agtggcgggt aaagttaaaa tatattgcca ttgcactttt tgtgattgac cttttaagta
    47281 ttacaggcaa caatgcaggt ggaaacattg ctcacatcgg tggtgccatc tttggcttta
    47341 tctatatcaa acaattaaaa aatggtcgtg atttaacagg atggcttcaa cgactgattg
    47401 agaggattac tcaaaagaaa aaaccatcaa ttaaagttgt ttatcgtaaa gacgcttctg
    47461 atgaatcgta tcacaacaaa aaagcaaaca atcaggaagc cattgacaaa attctggaca
    47521 aaatcagccg ttcaggatat gcaagtctca cttacgaaga aagagacata ctttttaaag
    47581 caagtaaaaa tcaggattaa tgctcaagtc tgcccttaac ttgttgctat ggggaataac
    47641 cataaccata gcaatagtaa ctttcatggc tgtagcagca gcttatatta gtcctgcaaa
    47701 aatttggttc cttgcactat tcggcttgct ttttatttac ctctatctga ttgcatttat
    47761 ctggatgcta accttatcat tcagacatag aaaaatgatg ttgataattt taattccatt
    47821 aattgcaggc ttaccggcat ttgtagctca ttggaatttc acctcgtcaa acaaaaatgc
    47881 tactggagaa aacacaataa aagttctttc atttaatgtg cgtgtgttcg atctttacaa
    47941 ctggacacac aatctggaaa caagaagtaa catttttaac tttctgaaaa aaagtgatgc
    48001 aggcatttac tgttttcagg aatattatac cagcaaatta gtgccattta ataatactga
    48061 ttccataaaa tcactgttag gaatagccta cgtacacaca ttactaccca taattgaacg
    48121 cgattctgac caatggggaa ttgctacatt cagtaaatat cccattctga ataaagaaac
    48181 tgtatttaca gacaaagaat ctgccaacgg ttgtattgct accgatttac tgattggaaa
    48241 agacacagtg agagtctata acatacacct gcagtcggtg agattggcaa aaaacgatta
    48301 tatttttgtg agtgagttca acaataaaga taacactcag aaaatgaaag gttcgagaca
    48361 aattgttcag cgtctgaaaa aagcatttat aaaaagagcg catcaggttg acaaagttac
    48421 tgctcatatt agtgcttgcc catataaagt tattgttgcc ggtgatttta acgacacacc
    48481 tgcttcttat gcctacaacc aaattagtaa tggtctgacg gatagttttc tcagctgtgg
    48541 gaaaggactg tcatcaacct atgcaggcat gtttcccgga cttagaattg actatatttt
    48601 acatagtaaa gacattaaat ccatagacta tattcgacca ttagtgcatt tatctgacca
    48661 ctaccctatt atttctaccc taaattttta atatttttat ttatgaattt gtagaatttt
    48721 tttttacttt tggaacaatt ctaatttttc atttacataa ccaagctatt ttcactaact
    48781 aaactgatta acttttttca attatgcgag taaaaatttc attcacccgc gacaatactt
    48841 caagtaacag tattccattg catcatcaaa aactaattta cgatagttta aaaactgttg
    48901 ttcatgatgt aggtggcaat atgaagcaac taaatttttc ttccctaaaa ggaacctcaa
    48961 aaattcagaa tggatttatg aagtttttat cttctaagat tacattagtg atatcaggca
    49021 gcaatgatga attgataact aatatcagca acagaatatt tgagaagaat tttatgccga
    49081 ttggcagaat gaatctattg cctaaaggaa aagaagttac tcctgatccg gagttcacta
    49141 cacaaatgcg atatctctgc atttctcctt tggtactttt tgatccgatt aaagatgcag
    49201 cacgaagcat cgagcagatt gatccaacat cacataactt ttccgacatc ctatttaaca
    49261 caactcttga tgccatggaa aagtcaggtg tgccggaagc tactataagt tcctatgcag
    49321 aatttgatgt gcaacccgat gctgagtata tgcaaaaagc tgcagcggca aacaaaaaat
    49381 ttgcacgtgt ttacagaagt gctgaaggca agaatatgat tgggtattta gttcctttta
    49441 cgctacatgc acatcctgat gtccaccgtt atgtttggca taatggaatt ggagtgatga
    49501 ctcatgaagg ctatggcatg cttgatttgg taaagtaatt agctgaaaac atttttattt
    49561 ataattttag tcaggaatat tattgctgat tttatgttgc tttgataaaa aaatatttca
    49621 taattattct tgcagttttc gttgtagtag ctggcggaat tatgctactt cgcgagtcaa
    49681 ttgtacgtaa taaagtaaat tctttttgta cttcattcca tactaaaacc ggtggcaata
    49741 tcaacatcgg aaaaattcaa ttcaaggggc ttcgagtaat tgaaatcgaa aacatcagtt
    49801 tggtttcacc tttaaatgac acactgttaa ataccgatac ttttatagtg cagctttcat
    49861 tcttaaaact aattcgcctg aaaaatcctg tttctaattt ttcggctcat ggcattaaaa
    49921 ttaaaattgc atcaggtgat tcaataagca actactcctt tcttttcaga aaatctaaga
    49981 ctgaaaatac agaaacatca acagcacaaa gtacacaggc atcaaaagtg tttacacttt
    50041 ggaataagct aacagacctg tccgacctaa gctttgatct ggagcgatgc aaaattacta
    50101 tcatcagaaa taaagaaaca agaaactttt attttccgga aatcatcaac cacagaggaa
    50161 attgccgttt tatagcttac agcaacgatc atttacaaac agaaaaatgg caggctggtt
    50221 ttaagattga tgatgataaa aacgaattgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50341 nnnnnnnnnn ntcaccatca ataaaatcca ttttaaaaca aaggctgcat acgacagaaa
    50401 acaacctcat cgctatgccc tttctttagc atttgatgaa agtgcagccg acttctttga
    50461 atctcttccc aaaggaattt tctatcactt taaagatttc aaagctaaag gcaggttgaa
    50521 attcgatttg aattttgcgc ttgatatgtc agcacccgat tcactgatat tcaattctgg
    50581 cttaacaggc aatcagttga caatagaaaa atatggagat gaaaattttt ctcgcatcaa
    50641 taacaccttt ctatttgatg cttatgatgg cgacaaactt cagagaagtt ttctaattgg
    50701 tcctgagaat agcgatttta ctcctctgaa tgaaatctgt cactatttgc aatatgctgt
    50761 actcacttca gaagaccctt cttttttcta tcatcgtggt tttattcctg atgcttttaa
    50821 agaaagtatg attgagaaca ttaaagcaaa aaggtttgtt cgcggtggta gcaccatcag
    50881 tatgcaactg gtaaaaaatg ttttcttgtc acgtgaaaaa acaatttcgc gtaagttgca
    50941 ggagatgctt attgtttggt taatagaaaa caaaggattg gtgagcaaag agcgtatgtt
    51001 tgaaatatat cttaatgcca ttgaatgggg ccccggcatt tatggaataa aagaagcatc
    51061 acaattctat ttcagtaaac ttccatcgca actcaatctt gcagagagta tttttcttgc
    51121 atcaataatc ccccgaccca aatactatcg ttattcattt gatgaacaag gtaacttaaa
    51181 accttatctt gcctcctact ataaaattgt gagcaataga atgctttcca aaacatggat
    51241 ttcgccaata gatactttca atcttcagcc tcaggtaaaa cttaaagggc cggcattgaa
    51301 aatagtaatg cctgctgata gcactatcac cgattcgctt caatggaatc agtcaaatga
    51361 attgttttaa aaacgtctaa agatactttg taatttcagg acttgtgggc aaaacactgg
    51421 agcgaaaacg atgaattaat tttccgtttt catcaattaa aaatttttca aagttccatt
    51481 ttatgccacc ggtaaaatct gcattaggtt gtgctataag ccagcggaaa agcggatgca
    51541 tatctctgcc tttaacagat acttttgcag ccataggaaa agttacatca taatttttct
    51601 tacagaactc ggcaatttcg ttattacttc ctggctcctg ccaaaggaaa ttatttgcgg
    51661 gaaatccaac aataaccaat ttgtccttgt atttttcaaa cagtttttcc aaatcactgt
    51721 attgtggcgt gtaaccacat ttagatgctg tattaacaat cagaattttc ttccctttaa
    51781 attgagagaa gtcaatctgt ccacctgaaa gcccatctac tttaaactca taaattgaag
    51841 tggcggttga agcaggacca aaaagaaatg caaacataat cagcaatagt ttcattgtat
    51901 tgtaattaac agatgtaaaa caaactcttt taaaaaaagt ttaacagagt gttccatata
    51961 aatcaaatgc ctcggcagaa gtaattttta tattggcaaa atcaccaaca cgcacataat
    52021 tttccttagc atctactaaa acttcattat ctacctccgg tgaatcgtat tctgttctgc
    52081 caataaaata gtttccttct ttcctgtcaa aaagcgtctt gaaaattttt ccaacttttt
    52141 tctggttgat attgtcagaa atattctgtt gaacctgcat caactcatct aatctgcgtt
    52201 gcttggttgt ttcatcaaca tcatcattca atgtgtaagc atgcgtgttt tcctcgtgtg
    52261 aataggtaaa aacgcctact ctctcaaatt gcatttcttc aagaaaacga attaaagttt
    52321 catgatcctg ctcggattct ccgggatagc ccgaaataaa tgttgttcga atggcaatat
    52381 ttggtatttt attacgtact gtgtctaaca atgcaattgt tttatctctt gtaattcctc
    52441 tgcgcatact ctttaacatg ttgtcacttg catgttgaag cggaatatct aaatacttgc
    52501 aaattttgtt ttctctttgc attacatcaa gcacatcaat tggaaaccct gaaggaaaag
    52561 catagtgcag gcgaatccac tcaataccat caaccgtgca taactgctcc atcaggcttg
    52621 caagttttct ttctttatat aggtctaaac cataaaaggt agagtcttgc gctatcagca
    52681 ataactcttt tgttcctttt gctgccaact gttttgcctc ctgaaccagt tcattaatag
    52741 gttttgaaat gtgattgcca cgcataagcg gaattgcaca aaacgaacat ggacggtcgc
    52801 aaccctcaga aattttcatg taggcaaaat gacgtggtgt tgtcaccaac cgttcgccaa
    52861 ttaattcatg tttataatct gctcctaaag tttttagcag taatggtaaa tctcgcgtac
    52921 caaaaatgtt gtcaattcct ttaatctctt tctctaaatc gggtttgtat ctttcagaaa
    52981 gacatccggt aacaatcaat ttttcaattt tccccttttc cttctctttg gcgtaatgta
    53041 gaattgtatc tattgactct tgttttgcat tatcaataaa tccacaggta tttataatta
    53101 caatttctga ttgtcgtttc gattcatgtt caacctctat ttcatttgca cgtaactgac
    53161 ccattaataa ttcggaatca actacgtttt tgctgcatcc cagagtgaca acgttaactt
    53221 ttaactgttt ggaggttttt gttctcatgc tttaaaaagt ttttttaaaa tcagtttcca
    53281 tataattgat tttaacaaca tcatcattaa tcagtgtatt gagaaaatct attaacctgg
    53341 catagctttt aacataggca tttgaagctt tactgaaata ttgcttcaac cctgttctaa
    53401 tttcaagctc aattactctc ggaaaatgat cggatgatat ataaccgaag tttacataat
    53461 catcaaagta ttccttctgt aatgagtcaa cagggaaatc tttcaaaaaa tttctgattg
    53521 ccttcttttg ctttgatttc aatttctttg ctatatatac tacacgcgat tttccgtaat
    53581 cagaaacccc actgattttc atactatcat ccttcaataa atattcataa gaggtaaact
    53641 gcaggtttct gttatctgaa acttttaaac gaaaagattt atcaatacgc tgtgcctgaa
    53701 tgctcatact gaacaaaaca aatacagcaa gtaaaatttt aaagggagag agcatgaaat
    53761 aatgatattg agttatttat aatggcgcaa agttatcaat tctgtacttg tgtagcaatg
    53821 acaataccat tacacataat acatcatgaa aattgcgtaa aataaaaaaa ggattgaaaa
    53881 atcctactcc aaaaaaaaat atttttttta actacttcgt ataattaggt tcattacttt
    53941 tagcattcaa ttttgagaac ctttaccagt atgtctttcc gaaatatttt acttgttgct
    54001 gcaattgtat tttcatcatg caaacaaagt gcacacttag taaaaacaga aactactacc
    54061 taccctctta ataaacccgg ctacactgaa gttgactcct caatcttaaa ctcttactca
    54121 acatacaaac acaccattga ctccataatg gatcgtgtga ttattgtgag tgaaatgagc
    54181 atgcagaaaa aaactcctga aggtttgctt ggcaatttca ctgctgatgc agctttaaaa
    54241 caagtggaaa acaattttca ttctaaagat ttttctaaaa tagatttctg ctttttgaat
    54301 aatggcggtt tgcgtgcagc attgccaaaa ggagatatta ctattggcaa catctactct
    54361 ttaatgcctt ttgaaaatga aatggtattg ctgacattaa atggagcaac aactttaaag
    54421 ttaatcaatt ttattgcagc taaaggtggc atgcctgttt ctggtttaac aatgcacatt
    54481 caggattcaa aggtaacgga agctaaaatt caaggaatgc ctttcgatag tacaaaaagt
    54541 tatgttgttg ctacatcaga ttatcttgcc aatggtggtg acaatctgtc ttttttagtt
    54601 gatgctgaaa agaaaattgt taccggatta aaacttcgtg acctgcttat tcaatatctt
    54661 gaagaaaaaa ataaaaaagg agaaaaacta tctgctaaaa ttgaagggag gattatttat
    54721 gacaaataga agagacttct taagaacatt tgcagtgggt gctgccggac tcagtctcac
    54781 cggattacca cttgaggtat tggccaaaag aaaggttact aagttaacta tacttcatac
    54841 taacgatgtt cacagccaca tagatccatt tcctgctaac gatccaaaat atccgggtct
    54901 tggtggagtt gcgcaacgtg ccgctattat taatgatatc agaaataaag aacgtaatgt
    54961 actacttctt gatgccggtg atatttttca gggcacacca tactttaatt tatttggtgg
    55021 tgaagtagaa ttaaaactga tgtcgcaact tggatatgat gccggcacca ttggcaatca
    55081 cgactttgac aatggtgttg atggactatc aagtcagttg aaacatgcca atttccctct
    55141 gataatttgt aattacgatt tcaaaggcac ttcgatggaa ggtaaaacaa taccctataa
    55201 aattttcgaa aaagaagatg tgcgtattgg agtatttggt cttggtattg aactggaagg
    55261 attggttgac aagaaaatgt atggtgaaac aaaatatctt gaccctttgg agaaagcagc
    55321 agaaacagca catcatttaa agtttaatga aaagtgtgat ttaattattt gtctctcaca
    55381 tttgggttat aggtatccaa ataaaaaggt aagtgatgat gttttggcta agaaatcact
    55441 caatatagat ttaataattg gtggtcatac acatacattc atagacaaac cattcgtgta
    55501 taaaaatcgc gacaataaag atgttttagt ggcacaggta ggttgggccg gaataaaatt
    55561 aggcagaata gatttttttg ttgataaaaa aatgcgtaaa ttgacagccg atagtaccac
    55621 gttaaaagtt ttgaattatg caagagcaat ttaatttttt tttcaagaat attcaaagca
    55681 tatttgcacc tcgattaaaa aacgagaacc accaaaaaat aatttttgaa ttttatctta
    55741 caattgaaaa tttacttcta tctttaaaaa ccaaatttta cctcaaacca atacttaact
    55801 tttaaaatta caattagtgt tgaaacagac agagaaaaat gttgaaaaag tgtgggaaaa
    55861 ttgtttgaag cttatacgcg acaatgtaag cccacaaaat tttaaaacat ggtttgaacc
    55921 catcaaaccg gttaaactag aaaagcatgt actgaccatt gaagtaccca gccagtttgt
    55981 ttatgaatgg ctcgaagagc attatataca actgttgcgc aagagtatta aaaaagaatt
    56041 aggtgctgaa ggtcgtttgg agtactctat tctgatggag aataacatga acagctcaaa
    56101 accttatgca gtgaagattc ctgctaaagg aacaggcaac ttaaagaatc cacaggtagc
    56161 aatgccggtt agcttaaaca cggctataaa aaatccattc attgttcctg gattaaaaaa
    56221 gattgatgtt gactcacagc tgaatcctaa ctattctttc gacaacttta ttgaaggaga
    56281 ttgcaaccgt cttgcacgct ctgcaggtta tgccgttgcc aacaaacccg gtggtacttc
    56341 ttttaatcca ttattattat atggtggaac cggattagga aaaacacatc ttgctcatgc
    56401 catcggtatt gagataaaaa ataatttcca gaataagact gtgctttatg ttcctgccga
    56461 caggtttatg aatcagtttg ttgatgcagt taaaaacaat tcaccaaacg actttgtaca
    56521 tttttatcaa atgatggatg ttttaattat tgacgacatt catttccttg ccggtaaaga
    56581 aagaacacag gatgttttct tccacatctt taatcaccta catcaaaata acaaacaaat
    56641 aattcttact tctgataaag cacctgttga cttacaggga atagaacaac gtttattatc
    56701 acgctttaaa tggggtttgt ctgctgattt gcagactcct gatttagaaa cacgcattgc
    56761 catcctgaaa aggaaaatgt ataatgacgg aattgaattg cctactgaaa ttgttgaata
    56821 tatagcttat agtattacaa caaacatcag agagcttgaa ggtgcactca tctctatcat
    56881 tgcccagaca tcattaaata aaaagcctat cactcttgaa cttgccaagc aaatgattga
    56941 taagtttgtg aaaaatactg tacatgaaat atctattgac tatattcaaa aaatagtttg
    57001 tgattatttt gaattgcctt tagaaacatt aaaatcaaaa acgcgcaaac gtgaagttgt
    57061 tcaggcacgt cagttagcaa tgtatttttc taagagtctg acaaaatcat cgctttcagc
    57121 aattggcgca cattgtggtg gcaaagacca tgcaaccgtt ttgcatgcat gccgcacagt
    57181 gaacaatctg atggaaaccg ataaaaaatt caaaggctat atcagcgatc tgcaaaagcg
    57241 aataaatctt aatcacaaaa attaaggtga aaattctgat ggtttgccta ggcaacatct
    57301 gccgctcacc acttgccgaa ggcataatgc aacataagct aaatcaggca ggcattgctg
    57361 cacatgttga ttcggcaggt ctgataaact atcactccgg ggaattaccc gataaacgtt
    57421 caatagccgt tgccgccaaa tatggtattg accttacagc tcaaagggca cgtagtttta
    57481 ccaaagatga cattaaaaat tttgattaca tctttgcaat ggataacaat aactacgaaa
    57541 acctgatgga atatgccaat gatgcaacag aggcaaaacg cattcatctt attttagact
    57601 atgcagggtt aggcaaaaaa attgttcccg atccctacta tggcgatcaa agtgattttg
    57661 agcatgtatt tagcttactt gatgatgcct gccagcgcat tttgaaaaag tttgcacatg
    57721 ctcaatccga agttgtctga tataaaaaca aaaggagttt tataccttat tccaactgca
    57781 attggcagta tgccggcaat agattacctg ccacaaactg tcattgaaac tattcattca
    57841 ttagatgagt ttatagtgga gaattcaaaa tcagcaagac aatttcttaa atatatcaat
    57901 atcccaacac cacagcaaca actcatcatt catgaactta acaagcatga aggcaatgtt
    57961 tttgaaacaa cttttttcag caatctgctc tccggaaaaa atgttggtct gttgagcgaa
    58021 gcaggctgcc ctgctgttgc tgatccgggt gcattaattg ttgcagaagc acataaacta
    58081 caaataaagg tagtcccact gactggaccc tcatccatac ttttagcact aatggcttca
    58141 ggactcaatg ggcaacagtt tgcatttaat ggctatcttc ctaaggaaaa aacagagcgt
    58201 aatgctgcaa ttaaaaaagc ggagaaagat tctgcacaac gcagacaaac acaaattttt
    58261 attgaaacac cctatcgcaa caatgccctg tttgatgatt tgctaaataa tctgcaacct
    58321 tctacccgac tttgcattgc ctcagcacta caatgtgaaa atgaaatgat taaaacacaa
    58381 agagtttctg aatggaaaaa acaacgacag gagttgaaca aacaccctgt agtttggtta
    58441 tttttggctt aatacattta gaatggttgc gctgctgaac tttctactca ttaccataat
    58501 ctcatgcttg gtagtatcgt tgattttact catggtaagc ccacgcattc cattgtttat
    58561 tagttacctt gccattgccc tgcacattct ttccattgca ctgtatttga tttatcgaaa
    58621 ttcaatacac gaggaaagag ttgtcatgct ttttgcttta attatatgta gtggcatcat
    58681 accgtttacg ctcattgtca gacgaaattt tccaatcata ttaaaaatat actttggaat
    58741 ttttatgctg agtttgccgt tgtttataat tgctccatct agaatgctca ctattctgag
    58801 tatggggtat ctgaacacag aaaatgcaca tgaaatgctt cttgataaga actactacct
    58861 cttgaaagaa cagggattga ttaaacaaaa cgataatgaa gctacctaca aagttgttaa
    58921 aagaatgggc atctttaata aaactcttgc acgtaatatt tttattggtt ttaggccgga
    58981 ttcagcaaaa attttatttt tagacgaaaa atcggaaatt cgcctccgtg cttttaaata
    59041 caataaaaca gaaattgata gcttagatat aacaacaaaa actgtatcat ctcgcgacac
    59101 cattttaaaa attattaaaa ctcaacataa atgacacgac tctttttatc agtatttgca
    59161 ataatttcag tactgacagc atctgctcaa caaatgtcaa cagatatctt tctgacaagt
    59221 tttggcatga accgcgaagg acaatatttt tttactgctc cgcaaaacat tacacatcgt
    59281 ccgggctacg acaatcaacc ttcgttttct aaagacggca gtaaagtata ttatgtagcc
    59341 tattacgata ctttgcagag tgatatctat gtttataatc ttgaagacag cagcaacaca
    59401 aggctgacag agactcctga aagtgagttt tcacctcgcg ttactgctga tgatttagga
    59461 tttactgttg tgagagttga tgcagataaa ggtcagcgtt tgtacaaaat actaatggat
    59521 ggaacaaatg aatttcaact gcttggtgta tctgattctg ttgcctatta ctgtattgcc
    59581 aatgacagta cctatgctgt agccgactta aacaacaatg tcatggaatt aaatatttac
    59641 gaaatgcccg gtgaacaatt tattccactg gcaaaaaata ttggccgctg tattgcagta
    59701 attcctgatg gtgataatga aataagttat gttgacaaag cagacaccaa tggttatatg
    59761 ctgatgtcat tttcaataaa ctcaggatta attggaagtg tatgtcaact taaaaaaggt
    59821 gttgaagatt atgtgtggac aaaagatggt aaactgctta tcggctacga aggcaaattg
    59881 atgatgtttg accgcagtaa gcctgagaac gggtggacag aacttgcaga cttcagtaag
    59941 tctgttggga atttttacag aattgcaatc agtcaacaag gaactcaact ggcattggtt
    60001 gcctacacca aggaagataa gaaacaagat gccaccaaag atgataccgg caaaaaagat
    60061 gaaaagaaaa aacgtaaaaa gaagaaagat gaataaagcc gaatttgtta ttgaacgcct
    60121 gtagcattgt gttcaactct cttttaagca tcagatttac gaattaaatt gctatgacaa
    60181 gaacaaacct tcccacatga aaactgattt tatcatagtc ggaaatggtt tagccggaag
    60241 tctgcttgcc tggcagtttt tattacaaga aaaaaaggtt gttataattg ataagttcaa
    60301 caactctgtt gcatcacagg tttcagcagg aattttttta ccgattacag gtaggcgcat
    60361 tgtaagaacg tggatggctg acgaactact tgctgaagta aaaacacttt ttacagagtt
    60421 aggaaaatta aatggcgaaa catatttaca tgaaatgcct gtagttgaaa ttcctgcttc
    60481 cgttaaagaa tggaatgaat ggcaacagcg tgcatcaagc ccagacatca gcaaatacat
    60541 cagtcgtttt ttagaaccta atgccattga atctattcat tgtccatacg gtgcaattga
    60601 attaaaaaac agtggttatc taaacatttc taaatgtgtt aattttttaa ggaaattata
    60661 tttggataag gaaatattaa ttgaagaaaa attcaacttt gctaatttac agtattccga
    60721 taaaataaat tataaagatg ttaaggccga taaaataatt ttctgtgaag gtgcagaagg
    60781 tgttaacaat ccatttttta attatctgcc atttcaatta tccaaaggag aaatactgac
    60841 cataagttgt aatacacttt cagaaaaaca gattttaaat catggtattt ttattctgcc
    60901 aataggcaat cagttattca gagtgggatc aacctacgaa tggaatgatt taacaacaaa
    60961 cacaacagaa agtgccagaa ataatctaaa tgaaaaattg caggcagtat taaaaacaga
    61021 ttttgaaatt attgagcata aagccggcat cagaccaagt gtaaaagaaa gaagaccttt
    61081 tatcggtatg catccaaagc ataaaaacat tggaattttt aatggactag gcactaaagg
    61141 agtaatgctt gcaccttatt tttcaaaaca ttttgcctgg catctggcac atgaaaaacc
    61201 gctaatggca gatgtggata tcgctcgttt ttcttaaact atttttcttt cttacgtctt
    61261 tctatttcct gtgtaatcat acttagttcg cgccccatct gaccactcat gctggtattt
    61321 tcagcagcac gtctgaataa gtagggcaac acggacacta caggcccata aggcacgtac
    61381 ttacaaacac agtaacctgc attggctaga ttaaaactga tgtgatcact catacctaac
    61441 agctgtgaaa accaaatacg ttcatcagaa tttgcaatgc ccttctcttc cataagtttt
    61501 gcaagtaagg cactgctttc ttcattgtga gtacctgcac aaacagcaac catgtctata
    61561 ttttcaatac acaatttgat agcttcattg tagtctttat cacatgctgc tttatcaggt
    61621 tgtattggtg aaggataacc attcagtgca gcacgttctc tttctttttc catataggca
    61681 ccacgcacta acttatatcc aattttgtaa ttacctgaac gggctttagc aatattttct
    61741 ttcataaatg caaccctgtc atgacgataa agctgaactg tattgaaaat aagcgttttc
    61801 tctttgttgt atttcaacat catgtcatca accatctgat caatggcatc ctgaatccat
    61861 gtttcttctg catcaataaa caaacgcaca ttattctctg ctgctgtctt acaaattgat
    61921 tcaactctgg cttttaatct ttggaattct gcatcttcag ctcctgtaag cagtacctta
    61981 ctgctgacac gctctaacaa atccattctg ccaacaccgg taaccttaaa cacagagaaa
    62041 ggaatatttt tctttccgct tgcatgtttt acggttttaa taatctctgc acagctatga
    62101 tcaaaatcag attcattgtg atgtccttct acagaataat ccaatataga acctactcca
    62161 tattttccaa gattatttat tgtttctgtg cattgttcaa tggtttctcc tccgcaaaaa
    62221 tgtttgtaaa tggtagccct gattaatggt tttatgggca aacccatttt caaaactgca
    62281 ttggtgaatc ccggaccaat ttttaccaga aaattaatat tgatgagcct gaataaccaa
    62341 taggctcttt taagatctgc atcgctctta taagcaaatg caaccttcag attgttgaat
    62401 gatacggatg gcatattaaa aattctttgc caaagataat tgaattaaaa tatccgtaaa
    62461 tctaatttct gcaagaggta aatatgatat taatcaacag tttaacattt tgttttaacc
    62521 ataccttaac caatctgtca aactctgttt atgggcatgg aaagctattt gtgataaaag
    62581 ctaaatacac tttttgaaat aaaggtagaa taatatcact ccatcagctc tttcaatttc
    62641 ctttccaact catctggcga aaggtttgaa taaaactgtc caccggcagc aatagaaatc
    62701 tgcccgtttt cttccgatac taccaaggct attgcatcac tctgttctgt aatacctaaa
    62761 gctgctctgt gacgcattcc gtaatgtgca gggacatcta aactttcagt gacaggcaaa
    62821 acacagcgtg cagcacgaat tctgttgcca cttattataa cagcaccatc atgcaatggg
    62881 ctatttttaa agaatatact ctctaaaagt cgtttggaaa cttcggcttc cataaaatca
    62941 ccggtacctt caaacaaatt caaatcggtt tttctggtaa caactatcag agctcccgtt
    63001 tttgttttcg acatggaaac gcaggcacga acaatgggca tgacacttaa ttcaaccggt
    63061 tgttgtagtt tgaaatttcc atgtaaaaga ttcttaaaaa aatcgccacg ggtaaaaata
    63121 gtattggagc ctacaaaaat taaaaacctg cggagctctt gctgaaaaac aatgataagt
    63181 gctatgacac caactccgat aaattgtccg aacaatgcac caagcaattg catgtcgagt
    63241 actttcacaa aaagccacca cagaaaatag atggtaagaa tgccgataag aatattcata
    63301 gcaacaccac ctttcagcaa acggtaaagc ttatacaaca cccatgctac aattaataca
    63361 tcaattatat cgagcagcct tactgttaaa aatcctatgt gaaacaactc catacctgtg
    63421 gctatgatta caaataacag aagtttattt caacattact gttgtttcat gatttttttc
    63481 gcacaatgtt gttgacatta cattttaagc tgctaataaa tactttgaaa aaaagaaaac
    63541 aactaagttt gcaatgctct taatcgctgt agcaatggcg gatgcgagta ataaaagaaa
    63601 acatacatgg gatgcggagt aaggtttgac aaactatctg aactgagttt ttttaatgca
    63661 cttgccaatg cttctgcact gaatgttact cttgcaaaat tatcagcttc aaactcattc
    63721 tttctggaat aaatattcat aaggattcct gtaataagtg ataaaggtga atacaataaa
    63781 gtaaatgcaa ttagacttat agacagacta tactctgttc cacccaaagc taatgaaaac
    63841 cattcattat tgataaatac agagagcaac cacaacatta aagcagtttg aagtattgaa
    63901 aatatcattg aaaacagagt atgttttttc ttgtaatgtc ccacttcatg agccagtaca
    63961 gcaaccaact catctttcga ataattattt accaaggtgt caaaaagaac aatctttttc
    64021 tttccgccta atccactgaa aaatgcattt gcctttgatg agcgtctgct tccatccatc
    64081 acatagaggt tagcaacttc aaatttattt tcatgacagt agttctcaat tgcagttcgc
    64141 acttcgccag cagccaaagg agtaagttta ttgaacaatg gaagaattaa cgaagtgtaa
    64201 aacatattca gcaccaccat aaatattgca aaaactgcaa gcatgtaaat ccaaaaatca
    64261 cttcccgtgt attgataaaa taaaacaaat aaggccaaca aacttccgcc taatgctcct
    64321 gcaagaaaat atcctttaat tctgtcagaa ataaaaacag caagtgttgt tttattaaac
    64381 ccataacgtg cctcaattct aaaggtagaa taaagtgaaa atggaatact taacaaatca
    64441 ctgaacaatg caaaagttcc aaaaaacagc aaggtggacc ataataaatc tgtagtgatg
    64501 ctatgcaccc aaccatcaag ccagccatat ccaccaagaa tcagaaaaaa gaaagaaatg
    64561 ataaacatta cagtttcact aatcattcca aagcgacttt tctcccgctc atattgttgt
    64621 gattttttat accgctcttc gtcataatat tgttttagct catccggctg cgtttctgat
    64681 aagttaagta aatttaaaat agacaaactc atactcaaca gataattcaa tacaagtatt
    64741 gatgcaatta tccataacaa tagttctttt aattccatgc tttattttta aatttatatc
    64801 ctattcccca ttctataaaa tcggctcttg caatatgtgt ttttaagttg atgcaagcaa
    64861 acaacccatc ataaaaacaa taacgcatcc ccaatctgtt ataaaacaaa ccatcttttt
    64921 tccatgcatt ttttgcataa acacccattt gcagcacaac agaaaaatct gaaaaaatta
    64981 tttcatacgc acctgcaaca cctccacgaa aatttgaaat tgaaccttgc gttttcatgt
    65041 cttcatactc aagtcggtaa ttaattgtcg ggtcgtagaa catatctaca cttatgcctg
    65101 ctgctgattt aggactgtac tgtcttaaga atgcaggaga aagtgtccat gcataccagg
    65161 tgcgccctgc aagtggataa acctgtttga aagcccatgc actaaaaatt gtgaagcgat
    65221 ttcttttttc aaaatttacc ggctcatggc tgataatttt ttttgactca ccaaaactat
    65281 ggctgtaatg tagttgtgcc gaaattaaat taattcctaa gttgggcatt gctgcacttc
    65341 catttgaaaa atgcgtcagc cctatggcag gaacaatcct atcacgcttc gttagccaaa
    65401 actctgagtg aaagttaaat gctatgacag cattgagatg tgacccgatg gccacattct
    65461 gataattttt atttctgtca taaggcttgg taatatatcc taatccccat ccgaactgaa
    65521 aatgtaaaaa tggcttctgt gacttaccta aaggaatgtc aatataagga taaacggtat
    65581 gcccatagcc taactgttct ttatttccta atccgaaaaa gccatattta attcctgttt
    65641 caggaaaatg aaagtgctga tgccatgacc gtttgccgtc tgcctgatgt gacaatgtaa
    65701 tcatcatacc accaaggtgg ttatgttgca gtggttgtac tgaaggccgg tgagccatga
    65761 caaaaccata atgcccctct gcacccaaca tccaggaacc gtgtgtgacc tgttgtccat
    65821 gcagcgtaag tgtaagcata aataaaaata gtgtggacag aaaactaatg cctgctttat
    65881 gaatcatacc tttgcaaaga aaatgctttt agcaatggaa gaaaaattaa atgcctttaa
    65941 aagaatttgt acaatcatgg acgagcttcg tacgcaatgt ccatgggata aaaagcaaac
    66001 catagaatcg ttgcgccatc tcactattga agaggtttat gagttgagtg atgccatctt
    66061 agataataat ttggaagagg ttaaaaaaga attgggtgac attatgttac atttaatttt
    66121 ttattcgcgc attgcagaag aaaaaaacct gtttaatatt gctgatgtta tcaatacaga
    66181 atgtgagaaa ctgatttcgc gacatccgca tatttatccc tctgccgatg gaaaaaaaat
    66241 tgctgttgcc gatgaagaag aagtaaaacg taactgggaa aaattgaaaa tgaaagaagg
    66301 taaacaatct gtattggaag gtgtgcctcg ttcattaccc agcatggtaa aagcacaacg
    66361 cattcaggag aaagctcgcg gtgccggttt cgactgggaa aaacctgagc aggtatggga
    66421 aaaagtagaa gaagaattaa acgaatttca ggaagctgtt tcgcgtaaag accaaaaaga
    66481 tatgtctgat gaattgggag atgtgttttt cgccttaata aattatgcac gttttcataa
    66541 tttgaatccc gatgaagcat tagagcgcac aaacagaaaa tttatttcgc gtttcaggta
    66601 catggaagat aaggttaggg aatcaggaaa aactttacat gaactatcgt tagcagaaat
    66661 ggatgtttat tggaaccagg ccaaaaccac agaaatctga tgatgcatat tgatattata
    66721 acagtacatc ccggtttgct tgaaggccct ttcagtcatt ctattctaaa aagagccatt
    66781 cagaaaaatc ttgttgaaat aaatatcatt aaccttcgcg actatgccac caacaaacat
    66841 aaaagtgttg acgactatgc ttttggcggt ggtgccggta tggtgatgat gatagaaccc
    66901 attgaccact gtatagcaga cctaaagtcg aaaagaactt atgatgaagt catctacatg
    66961 tcgcccgatg gcgaacaact caatcaggga ataagcaata gcctctcact gaaaaaaaat
    67021 attattatcc tctgtggtca ttacaaaggt gttgatgaaa gagtgcggca gcatcttatt
    67081 acacgcgaaa tttctgtggg cgattatgta ctttcaggtg gagaacttcc tgcagcagtg
    67141 ctttgcgata gcatcatcag aattattccg ggagtattaa atgatgaaac ctctgccctg
    67201 tctgactctt ttcaggataa cctgctggca ccaccagttt atacccgacc ggcaaattac
    67261 aaaggctggg atgttcctga agttctttta tccggtaatg aaagccttat taatgaatgg
    67321 cggcacctac aaagcctgga acgtacccaa ataagaagac cggacttatc tgacgacaac
    67381 agcaaataac tgtttttcaa taaaatatgg ttttttaaat aaattttaaa aagttaggag
    67441 aaatcttatt tccctaaagc tttttttact attattgcac tcccaaaatt tatcatcatc
    67501 aaaacattaa agcgatggat ttagttaaat tagcagaaaa acagttaata ccggtaaaag
    67561 atactccgga tttcaaagct ggtgatacca ttaccgtttt ctataaaatt attgaaggta
    67621 ataaagagcg cgagcagcag tttcagggag tagttattca acgtaaagga catggagcta
    67681 cacaaacatt taccgttcgt aaaatttcta atggtgtagg tgttgaacgt atttttccat
    67741 tgtacagccc taaaattact aaaattgatg taaacaaacg cggtgttgtt cgtagagcaa
    67801 gaatctatta cctgcgtaat gtttcaggta aggctgcacg tattgaagaa aaaattgtta
    67861 agacaggtga gaccaagaaa aaataaaaaa gtttcatttt gatttgtaaa aaaagcctcg
    67921 tcaataagcg
//
LOCUS       DAOQNP010000005        62088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_126_length_62088_cov_7.768978, whole genome shotgun sequence.
ACCESSION   DAOQNP010000005 DAOQNP010000000
VERSION     DAOQNP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 62088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62088
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_62088_cov_7.768978"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1686)
                     /gene="guaB"
                     /locus_tag="PKY09_01345"
     CDS             complement(217..1686)
                     /gene="guaB"
                     /locus_tag="PKY09_01345"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNB11525.1"
                     /translation="MALASKSNLQEGLTYDDVLLVPAFSEILPRDVDTSTYFSRNIKL
                     NVPIVSAAMDTVTESAMAIAIAQEGGIGVLHKNMTIEQQTNEVRKVKRSESGMIHDPI
                     TLHQNATIHEALGIMREQRIGGIPIVNDKNKLVGILTNRDLRFETDESRKVKEVMTSG
                     QLITVTGVVSLQKAEVILRQYKIEKLPVVDRSGKLIGLVTYKDIIKFKSRPNSCKDKL
                     GRLRVAAGIGVTADMLDRVSSLVNAGVDAIVIDTAHGHSKGVITALKSVKKKFSGLDV
                     IVGNVGTAEGAKLLVKNGADGVKVGIGPGSICTTRVVAGVGVPQLTAVADAASAIKGS
                     GVPIIADGGIRFTGDIPKAIAAGANSIMAGSMFAGVEESPGETIIFEGRKFKSYRGMG
                     SIEAMREGSKDRYFQDAEDDIKKLVPEGIVGRVPYKGTLAETVYQLVGGLRAGMGYCG
                     AKNIADLQKAKFIRITQSGVRESHPHGVTIVKEAPNYTR"
     gene            complement(1749..2339)
                     /locus_tag="PKY09_01350"
     CDS             complement(1749..2339)
                     /locus_tag="PKY09_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNB11526.1"
                     /translation="MKFSVWLCFLFTAFIKFSNGQTPVGSSTAVYNGDTIAYYTLNAV
                     DIYGFIPANASDDVRKYMKLRRDVLKAYPYAKIASAELRFIEESVAKMTSEKEKKKFI
                     KEQEKIMKEQFEKDLKNLTYTQGKILIKLIDRETGHTSYNLVKDLRGSFQAFFWQGIA
                     KIFSANLKSEYDPDNADKMIEKVVQSIEKGELQVRN"
     gene            complement(2417..3898)
                     /gene="accC"
                     /locus_tag="PKY09_01355"
     CDS             complement(2417..3898)
                     /gene="accC"
                     /locus_tag="PKY09_01355"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB11527.1"
                     /translation="MFKKILIANRGEIALRVMRTACDMGIKTVAVYSDADRMAPHVLC
                     ADEAVHIGASSSAQSYLLVEKIIQAAKLTGAEAIHPGYGFLSENADAAQAIINAGLVF
                     IGPSPQSMRTMGSKLGAKEAAKKFNVPLVPGTDYAISDIEKARQIAVDIGFPVLIKAS
                     AGGGGKGMRIVESEKAFNESVKSAMSEAQSAFGDGSVFIEKYIASPRHIEIQVMGDNH
                     GNVVHLFERECSVQRRHQKVIEEAPSGVLDEKTRAAMGQCAVMVAKACDYSGAGTVEF
                     LLDEMNNFYFLEMNTRLQVEHPVTEMITGIDLVREQLHVAAGNKLSFSQEDLKIHGHA
                     VEVRVYAEDPANNFLPSVGKLSTYITPRGVGVRVDDGYEQGMEIPIYYDPMIAKLITY
                     GRDRDEAIARMIRAIDEYKITGCETTLPFCRFVMQHNAFVTAKFDTHFVKKYFTPEVL
                     EKTIADEAEIASLAAQLWLEEKKPAAIQQQQEKTISYWRNNRS"
     gene            4077..5693
                     /gene="pckA"
                     /locus_tag="PKY09_01360"
     CDS             4077..5693
                     /gene="pckA"
                     /locus_tag="PKY09_01360"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNB11528.1"
                     /translation="MNEYGLRNPNFSVAELGLKNVAAAYWNLTVAELVEEVVIRGEGE
                     LTDFGALAVDTGEFTGRSPKDKFIVADKETENSVWWGDINIKFDADKFDALYNRVTAY
                     LSGKEIYVRDCYACADPRYRLNIRVITESPYQSIFSNNLFLRPTPEEITTMEKDWVIM
                     AASGFMADPKIDGTRQHNFAIVNFTKKIILIGGTGYTGEIKKGIFSVLNYILPHHKNV
                     LSMHCSANIGKNNDTAIFFGLSGTGKTTLSADPNRGLIGDDEHGWSDNTVFNFEGGCY
                     AKCVNLSKEKEPQIYQAIRFGALLENTEFFEGTTRVDFDNISKTENTRVAYPIHFIDN
                     AVEPSVAGIPKNIFFLTCDAFGVLPPISRLTTGQAMYHFISGYTAKVAGTEVGITEPT
                     TTFSACFGKAFLPLHPTKYAELLGKKLSENKNIRVWLVNTGWSGGSYGVGSRIKLAYT
                     RAIITAALNGELDNVKFETLPVFGLQYPVSCPNVPAEILNPKATWADKIAFDNKSADL
                     AAKFVKNFEQYASHANKEIMDAAPKVAVVA"
     gene            6080..6916
                     /locus_tag="PKY09_01365"
     CDS             6080..6916
                     /locus_tag="PKY09_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11529.1"
                     /translation="MKKLFTLIFIAIAGLVVADILNEQGQAVATGSPGEFDCTSCHTG
                     NSVNSGTGSITISSPNMPNWQYVPGQVYQIDVTVAQTGAPLFGFGFEALRTSNNTNGG
                     TFQITNSASTFLKTGFINGSNRTNVVHKKNGGLSNDLHTFSFNWTAPATNIGNIIFYS
                     AGNAANNQGDTLGDFIYITQQLITPSITGLNEIKTPAISVYPNPATDFINVTLPESQT
                     ETSYSIKDLSGKIVDQGKTFNPLGKSFSVNLSETFKEGIYFIEVNNGVDHYIQKFVKL
                     KN"
     gene            complement(6945..8240)
                     /locus_tag="PKY09_01370"
     CDS             complement(6945..8240)
                     /locus_tag="PKY09_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNB11530.1"
                     /translation="MKKLLTILSAILLLPVWALSQSPALVGYWHNWDITSAPYIMLDS
                     VDSRYNIIDVAFAVPQAGTDYQMEFIPEQVSPAAFAMQVQNLQNQGRKVIISMGGATA
                     PVSLDNTVERDSFVSSMTSIINFYGFDGIDIDFEGSSVSVSGGTIASPVDAKIINLID
                     AVKQIMNNYYATYGKKMMLTMAPETAFVQGGMSAFGSIWGAYLPVIDALRDSLEILHV
                     QLYNSGTMFGIDGNIYTQGTADFIVAMTEAVIHGFNTSGGMFAGLPANKVAVGLPACT
                     NAAGGGYTDTASVKAALDYLLGKGPKPGTYTLVQSGGYPTLRGMMTWSINWDAVNNCS
                     SSSYQYADNFQKIFGTGTSLAEVNLQSIFVFPNPASDKIYISTNSDSNKPFYIYDMLG
                     KMIMSNHCSSTKQEVNISSFSNGIYYVKLENQILKFAKY"
     gene            complement(8305..8835)
                     /locus_tag="PKY09_01375"
     CDS             complement(8305..8835)
                     /locus_tag="PKY09_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11531.1"
                     /translation="MRKIHRIAIVGPESTGKSLLSEQLAYQYQTVWVPEIARDYLHKL
                     NRPYTFDDLSVIAKLQLEKEKSMSESAKHFLFCDTNLVVIKVWSDFKYGRTEKWIVEA
                     IQKSDYSMHLLTDIDIPWEDDPLREHPHFRKELFERYLQELNTFGLDYQIVNGIGESR
                     LKNALNHLEKLEPDLK"
     gene            complement(8819..9418)
                     /gene="pnuC"
                     /locus_tag="PKY09_01380"
     CDS             complement(8819..9418)
                     /gene="pnuC"
                     /locus_tag="PKY09_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNB11532.1"
                     /translation="MDLQTILFLNIPLIEWIGFLFGVVGIYLGIKEKIAYFPISLVNV
                     IVSLYIFYHQSLYSDVVQQGVYILLLSYGWYRWITGSQNTEMPVTNASKRLIFILLGG
                     IILLTVGMGSWFSHYTDAHLPYWDALATSMCFAAQYLMAEKKIENWLVWIDANILYIG
                     IYLYKGLPLYAILFSIYLIMAIIGYFKWQNHLIRHEENS"
     gene            complement(9422..10174)
                     /locus_tag="PKY09_01385"
     CDS             complement(9422..10174)
                     /locus_tag="PKY09_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNB11533.1"
                     /translation="MKQKILQQFQNHKKQLAVLIDPDKVNEKQLKVLCTYATAHQVDY
                     FFVGGSILTHGSIPETIAILKKFASLPVILFPGNASQVDPQADAILYLSLISGRNADL
                     LIGQHVLSAMSVKLSGLEAIPTGYILIDSGNVTTVQYVSNTKPIPHDHNELAVSTAVA
                     GQLLGMQVIYLEAGSGARRTVSGQMIEKVKKNTGLPLIVGGGIRTAEKAIEIYNSGAD
                     IIVVGNAIEDNPELMLEIAKAKSSINQTLNAL"
     gene            complement(10177..10806)
                     /locus_tag="PKY09_01390"
     CDS             complement(10177..10806)
                     /locus_tag="PKY09_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11534.1"
                     /translation="MPLIKSTKFDNSFTGIWQLTESNDMLSQMLRFYGKYNLPGHYTH
                     QRRIAEWYAVRLILHEIVPDKHFDIFYNEYGKPFLASPEGFISISHTHGFVGVLFHRF
                     NNCGFDMELMNRPVASVSHKFVRNDEEHFLHDNPLQKTLMLWSAKEVIYKIYGKKSLD
                     FKVNLRILPFEYLQEGKIKGQLILNNDICSYDIQYSFQDQLLTTFAIEK"
     gene            10886..12202
                     /gene="ahcY"
                     /locus_tag="PKY09_01395"
     CDS             10886..12202
                     /gene="ahcY"
                     /locus_tag="PKY09_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNB11535.1"
                     /translation="MSTVTDSKLKYKVKDISQAEWGRKEIKLAEAEMPGLMSLRAEFG
                     KQKPLKGARIAGCLHMTIQTAVLIETLKELGAEVTWSSCNIFSTQDHAAAAIAAAGIP
                     VYAWKGMNNEEFDWCIEQTLFAFEGGKPLNMILDDGGDLTNMVFDKFPELIKGIKGLS
                     EETTTGVHRLYERMQKGTLPIPAININDSVTKSKFDNKYGCRESLQDAIRRATDLMLA
                     GKVAVVAGYGDVGKGSAESLSSAGVRVIVTEIDPICALQAAMEGYEVKTMDNAIPEAD
                     IIVTATGNHNIIVDKHFKAMKHNAVVCNIGHFDNEIDMAWLNKNYGHTKDTIKPQVDK
                     YSLDGKDIIVLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQLELWNHTDKYENKVYTL
                     PKHLDEKVARLHLNKIGVELETLTQSQADYIGVKVEGPFKPDYYRY"
     gene            12368..14140
                     /locus_tag="PKY09_01400"
     CDS             12368..14140
                     /locus_tag="PKY09_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB11536.1"
                     /translation="MKILIQYLKRHWKLVLLALLLAAVNQCFSLLDPLIAGKMLDRFA
                     MHVSDYNDDFTNFFNAIIWFLLASMGVALVSRIAKNLQDYFVNVIVQRTGAEMYSDGI
                     KHSLELPYQVFEDQRSGETLGKLQKVRADSEKFINAFISILFQSVVGIIFVLAYAINV
                     HWSIAPVFLLTVPVLAIVSNMLSKKIKLISKKILGETTSLAGATTESLRNIELVKSLG
                     LANQEINRLNTVTDKILGLELKKVKYIRSLSFVQGTTVNFLRTLFVFLLYYLIFKDEI
                     SPGKMMTLMFYSFFIFGPLQELGNVIAIYREAQASLTTFSQILEMPLEHKPQSPHQLN
                     DIETFAFRDVSFKHISAPNNALSHINFTAKRGQTIAFVGPSGSGKSTLVKLLVGLYHP
                     ASGQILYNGTDSNSINLDDLRGQIGFVTQDTQLFAGTIRENLLFVNPQAADEQILNAL
                     KMASCNSLLERAENGLNTIIGEGGVKVSGGEKQRLSIARALLRNPKLLVLDEATSALD
                     SITEEEITETIKTINKNKNLITILIAHRLSTIMHADKIYVLEKGHKIEEGTHDSLLAE
                     KGLYYAMWRQQIGERKKGENVVTA"
     gene            14127..14747
                     /locus_tag="PKY09_01405"
     CDS             14127..14747
                     /locus_tag="PKY09_01405"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNB11537.1"
                     /translation="MSQLDNIKDFEVAISERLKDLPGSNSHIKLASVNRPVYEPMGNY
                     RTGSVMLLVNNKNNLPHLVMIKRAEDGKVHSGQIAFPGGKAESDESLTLTALRELEEE
                     IGIKNDSINIIGALTRVYIPPSNYMVYPFIATQINPSAFLANASEVQTIFEISLLSLI
                     ANKKIVKDVEHQTTRGLIKAPAFKIDNLVIWGATAMILSEFIDLID"
     gene            14815..16395
                     /locus_tag="PKY09_01410"
     CDS             14815..16395
                     /locus_tag="PKY09_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11538.1"
                     /translation="MRRHLQKLAAICMLGFFTMPTYSQRFLSEVFPSVNVTTNVVYAQ
                     NYEVLTGTPVLKDLKMDVYEPAGAADPMTERPLIIYLHTGSYIPIIVNGNATGSRNDS
                     AAVEICKQFARRGYVVANMDYRLGWDPTNTNPTNGLDIRRGTLLNAVYRSIQDAKSCV
                     RYFRKDAATNNNVYKIDVNNFYLVGQGTGGYIALNYAVLNSPSEITLPKFISSTDAPT
                     YGIYAGQPYVNQTALGDFDGIGGVAPYNNENNPGYSNAVQFVVNLGGAMGDSSWIAAG
                     DAPMIGIHCVDDPFAPYGVGIVYVPTTPPQAVVDVIGSGEIIKWANLLGNNACFANAG
                     FTDPYTIRANSINNGNEGLFPLQQGINPVNPQLGHAGPWEWYDLQSTRNLAMAMGYQS
                     TRGDTCYGNSMLTNPTMSKAYALAYIDTIMNYVNPRIVFCCNLPTSVKEVSTNSSDVT
                     IYNSPSKSYFTVVSTSKEPMRSIEIYDIKGSLLAAEKNPNAFSQRIEKVSLSLNKGVV
                     LVKITYNSDVITKKITLD"
     gene            complement(16446..17354)
                     /locus_tag="PKY09_01415"
     CDS             complement(16446..17354)
                     /locus_tag="PKY09_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB11539.1"
                     /translation="MNSRNIEEINLNAFQLAHKGVSLHVLRLNLNDEISGGNKSYKLK
                     YNLQAAKEKGRNTLVTFGGAFSNHIAATARTCKLQNIKAVGIIRGEIITPMNNTLKRA
                     VNDGMKLIQVSRQWYRQIHDKDFYKSDAVKHLLFENNINPDGCYIVPEGGNNAEGYKG
                     CTEILNDINAGYDTVVCAVGTGTTLAGLAASISENKIAIGIAVVQAADSLTKNVQLHL
                     SESNSTAKYIIRHQYTFGGFGKSNAELKIFCELFTQKTNILIEPVYTGKLFYGIFDML
                     VKDQFNTGTKILAVHTGGCQYLIDEK"
     gene            17482..17658
                     /locus_tag="PKY09_01420"
     CDS             17482..17658
                     /locus_tag="PKY09_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HNB11540.1"
                     /translation="MAFTDKFEKDEYYLTPEGYIVFTEKYHLKRGYCCQSGCRHCPWN
                     YNAKKADKNNKQKL"
     gene            17728..19368
                     /locus_tag="PKY09_01425"
     CDS             17728..19368
                     /locus_tag="PKY09_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNB11541.1"
                     /translation="MAEKVNLEFNINEDAYKTHLIRLTERLNKIYEGGGKKNAAKQKE
                     KNKMLARERIAYLLDTDKPQFEVGAFAADGMYAEQGGCPSAGVVIVIGYISGKQCIVV
                     ANDATVKAGAWFPMTAKKNLRAQEISMENKLPIVYLVDSAGVFLPMQDEIFPDKEHFG
                     RIFRNNAVMSSMGIIQIAAIMGSCVAGGAYLPIMSDEALIVDKTGSVFLAGSYLVKSA
                     IGEDIDNETLGGATTHCEISGVTDNKFPDDASCLDYIKRLFSKIGTFDKAGFERAASK
                     EPAANPKEIYGLIPAENSKQYDMHEIINRLVDNSEFDEYKEKYGQSILCGTARIDGWS
                     VGIVANQRKVVKSKKGEMQFGGVIYSDSADKAARFIMNCNQRRIPLVFLQDVTGFMVG
                     SRSEHGGIIKDGAKLVSAMANSIVPKFTIIIGNSYGAGNYAMCGKAYDPRLIAAWPTG
                     QIAVMGGSQAAKTLLQIQVAALKAQGKEISEEDEKAQLKTITDRYNSQTSPYYAASRL
                     WVDAIIDPLDTRKMISMGIEMANHNPISKPYNVGVIQT"
     gene            complement(19443..19874)
                     /locus_tag="PKY09_01430"
     CDS             complement(19443..19874)
                     /locus_tag="PKY09_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11542.1"
                     /translation="MKRILLFIALASFSLTANSQANKTMNSWAELNNFHKVLTETYQP
                     SKDGNLKAIKAEYKQLMELAVALHQSKAPAGIDAAKMSDVTTRLAVACKELFGLIELN
                     ESDESITKKLGETNSIFTEIARMCESNDVKGHDHSDPNHKH"
     gene            19947..20492
                     /locus_tag="PKY09_01435"
     CDS             19947..20492
                     /locus_tag="PKY09_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB11543.1"
                     /translation="MSRFIYSFFFKLFGWKIKGNIPNELKKYVMIVAPHTSNIDFFVG
                     LATRSILRLDTKYLAKKELFRPPLGWIFYALGGYPVDRSKPGKLVEKVAEIYNNHDKF
                     SICITPEGTRKYAAEWKTGFHKIAMAVKTPVVMVAFDYRTKSVVIAPPFTPSDDTQGD
                     IEKIKVWYRQFEGKVPEYGVK"
     gene            20602..21138
                     /locus_tag="PKY09_01440"
     CDS             20602..21138
                     /locus_tag="PKY09_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNB11544.1"
                     /translation="MPTKIRLQRFGKKGQPFYHVVVADSRAPRDGKFIEKIGTYNPRT
                     IPATIDLNLDRALYWLGTGAQPTETTRAILSYKGVLYKNHLLGGVKKGALTQEQADAK
                     FSAWMENKNLKVLQHSQKVQSTKADDMKNRLVEESKKREVIAKAIAEKRAAANVAEAE
                     AAAEAPATDGGETPAENA"
     gene            21167..21685
                     /gene="rimM"
                     /locus_tag="PKY09_01445"
     CDS             21167..21685
                     /gene="rimM"
                     /locus_tag="PKY09_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNB11545.1"
                     /translation="MGFDEVTFFGTIVKTHGIKGHLVLRCYDNFKCKKNNPVFLFFRE
                     GDLLKQAAVSEIQFTYPFCRIKFADIADINEAETWLKKEVYIEKKALLQNEIPYKFEL
                     IGCSVTDSISGLIGNVIDIAEYPQQQIAIINANGKEVLIPLNKNFITRFNSTENNLVV
                     TLPDGLLDIYLQ"
     gene            21850..24279
                     /gene="ccsA"
                     /locus_tag="PKY09_01450"
     CDS             21850..24279
                     /gene="ccsA"
                     /locus_tag="PKY09_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNB11546.1"
                     /translation="MEIKYIGEQLIWGYIGNGFIFTAFISAIYAAVLYLASVRNVEKA
                     QWATPLARKFLYLHSISCFGIFFLLLYLIFNHRFEYYYVWQHSNMAMPMYYILACIWE
                     GQEGSFLLWMLWHSLIALFISRRNRHWESPVVGTIMMVQVFLGSMLLGVYIFGYKLGS
                     NPFTLLRDNDQFANLPFIKMPDYLSRIADGRGLNPLLQNYWMVIHPPTLFLGFASTVV
                     PFAFALGGLMRKNITEWIKPALPYAFFSIMTLGAGILMGAAWAYEALSFGGFWAWDPV
                     ENASLVPWLVMVGAGHVMLVFNKRGRALLSTYILCILSFLLVLYSTFLTRSGILGETS
                     VHAFTDLGMSGQLIFYMLFFVVLAIVMLAINWKKIPKENSDDSITSREFWMFIGMLVL
                     CISAIQITFTTSIPVINKIFGSNLAPPVEVKEHFNSWQMPIAMIIALLMAVGQFFKYK
                     ESNPKEVFKKLLLSLTISLILAVVLILLLKFNSIHYMILLFTAVFAIIANFDYFIRVL
                     KGKFSFSGASIAHIGFAFILLGTLISNANSRIISKNRLNIDLGKDFPNNENIMLAKGD
                     TLQMGKYFVTYSGREKKGHNIYFDVEYYKLNNKTGELKKAFSLKPIIQLNERMGNSPE
                     PATKWFPDKDIYTHVTYANLDEYTKNVSMDEYDTPTEHEMAVGDTLATSNSLVVLEKL
                     VKDNTGVEVESFDSFGVTALLEVTDVNKKKHTITPQFLIKDRMAVSKNYFLDELGLKF
                     RFTSISPESGKIKLEIAEKKANKKDFIIMKAIIFPHINLLWIGCILMLTGTWIVSVKR
                     FKEYVRYTDKG"
     gene            24254..25045
                     /locus_tag="PKY09_01455"
     CDS             24254..25045
                     /locus_tag="PKY09_01455"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNB11547.1"
                     /translation="MSATPTKDNSMTLTIIGSGNVAWLLAILFKTSGHKINQIYSRNK
                     KTGSALSKITGSKFIADVQKIDIKSDIFIIATNDDSIAEVVSKLPDINSIFVHTSGTV
                     DLQVLKKKFKKAGVVYPLQSVASESVLSDELTFCLEATNNRVLTSLKQLITSINCGYV
                     VMNSQKRAKLHLAAVLVNNFTNHLYAQAFDFLKKNRIEFNLLKPLIRHTVMRLDGNPA
                     SFQTGPAVRNDKKTIQRHLMLLKDNKQLTKLYKQMTDSIYKFHKK"
     gene            25042..25548
                     /locus_tag="PKY09_01460"
     CDS             25042..25548
                     /locus_tag="PKY09_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNB11548.1"
                     /translation="MNSVNFKEKLKDIKCFVFDVDGVLTNGMLHVTNDGMLLRAMNIK
                     DGYALQLAVKKGYRVVIISGGKDEGVVIRLKNLGITDIYVGIGNKLQQLLAVMQEHHL
                     TSAQLLYMGDDMPDVEAMKNCSIATCPYDAAPQIRDICHYISPIKGGDGCARDVIEQV
                     LTLNNDWE"
     gene            25548..26492
                     /locus_tag="PKY09_01465"
     CDS             25548..26492
                     /locus_tag="PKY09_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNB11549.1"
                     /translation="MGFLKLIRFPNLIILALAQCLIYYMVVVPLLKTSEFTPSLHTWQ
                     FLLIVLSTVLIAAGGYVINDFFDKDIDAANGIGKVHDFSGWTMKTLYFVFSIIGIAIG
                     LYLTYALKLRQFALTYLLTAALLYFYSASYKRLPLVGNFVVAFLTAVAVFLPAFADYE
                     LQYAFRDIKLPVINNKMYNLRLIIAIAAAYAFFAFLISLVREIIKDMEDIEGDREFGC
                     NTLPIVAGINNTKLIAIGLLLLIVGLILFIQIKQAWWESLPVFGYTILFVQVPLLILS
                     VKLFLSAEKKHFKVASMIAKVVMVGGILSLPVFKFFST"
     gene            26507..27814
                     /locus_tag="PKY09_01470"
     CDS             26507..27814
                     /locus_tag="PKY09_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB11550.1"
                     /translation="MMLLVYTHKFSPRLKYIFDLFIGKLMGISFEITHNVEEFKSYAG
                     PKFNYSEHQIENEIFIYSERLLFEKGIEDQQFGFTEWDGIKAFYATHPKYSVPFDLFS
                     ASFYLVSRYEEYLPHLRDLHDRYNETESIAYTRGFLQKPVVNIWAQKFKSIILERYPT
                     LKSVSPKYKYVSTIDIDNAYAYLEKGLMRTIGAYGRSLVNFDLPQIVERSKVLMRLMH
                     DPYHTYELMHDLHKRYKINVIYFFLLGEYGENDKNVSVDNRNFQSLIQSLADYADAGI
                     HPSYGSNIKDGRLQKEVQLLTKILKREVTKSRQHFLKIRLPDTYRKLISVDIKEDFTM
                     GYAGNIGFRAGICSSFYFYDLDQEVQTPLLIHPFAVMDATFRYYLKVNPEQVIDLIKP
                     LIEEVKAVNGTFISLWHNESLSENHIWKGYRDIYEELLKIAAV"
     gene            27816..28727
                     /locus_tag="PKY09_01475"
     CDS             27816..28727
                     /locus_tag="PKY09_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11551.1"
                     /translation="MEIRYLEHSEIDKAKWDLNINQALNKLPYAFSWYLDVVSPNWHA
                     LVIDDYNYVFPLTWRKKMGFNYLYQPMFTQQLGIFSSHPVDYATSNAFLNAIPSQFKL
                     IEINLNSFNPAAGNKFIATKQLNFELELNKPYEELRKFYSENQKRNINKAKRSELSLN
                     QPDISEVISLFRHDRGLKIERMKDESYELLSRLFSTLKQKGICYTRGVSDGSQTICGG
                     LFIQTDYRIIFLFSGNSVIGKSSGAMSLLIDSVIQENANKNIVLDFEGSNDFGLARYY
                     SSFGSIEHVYLHLKKNTLPFPVRLLKR"
     gene            28730..29377
                     /gene="upp"
                     /locus_tag="PKY09_01480"
     CDS             28730..29377
                     /gene="upp"
                     /locus_tag="PKY09_01480"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNB11552.1"
                     /translation="MVNILSTSNSLLHHFVAELRDVEIQNDRMRFRRNLERIGEIAAY
                     EISKTMIFENTDVTTPLGIATVPLMQSQPVLGTILRAGLPLHQGLLNYFDQADNAFIS
                     AYRRHHKDGSFDIHLQYLSSPPIAGRILILSDPMLATGQSLVETYKAMLESGQPAHTH
                     VVAAIASRQGIEYVKANLPDDLTIWVAAVDEELTAQSYIVPGLGDAGDLAFGEKK"
     gene            29428..30009
                     /locus_tag="PKY09_01485"
     CDS             29428..30009
                     /locus_tag="PKY09_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11553.1"
                     /translation="MGEFVKILMVILISSVKFVVGPAFAYYDEKYDFTIFEAIVYPII
                     GGMLGVFVFSFFSDQVANVWHWLQNRIKKTVSRNQPFTEPTVDSEGKVVVSYTYVDAK
                     EKPKNKIFTKRNRRIITIWKKYGLFGIALLTPVIISIPIGTIIASRLVPDKKKVFLYM
                     LISISAWSIVMVSSFELYHAYSIKALQQKVLEP"
     gene            30006..30710
                     /locus_tag="PKY09_01490"
     CDS             30006..30710
                     /locus_tag="PKY09_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNB11554.1"
                     /translation="MKKLKHYSHLIFDLDHTLWDTNVNAAQSLSEMYHQYNLGQYGII
                     SEHDFIQKYVDINNRMWTEYSLGRIKKSYLRAGRFELTLKYFGIANKELVEKLSDFFV
                     QNTPGKKELIPFAKDLLNHVHGKYQLAIITNGFAEAQHTKLRASSIDHFFSKIIISEE
                     IGYHKPDPRIFLQTAELLQTPIKDCLMIGDNVETDIAGAIASGMDCAYLNPAGVKHNY
                     EVTFETHCLSELIKWL"
     gene            30715..31635
                     /gene="rsgA"
                     /locus_tag="PKY09_01495"
     CDS             30715..31635
                     /gene="rsgA"
                     /locus_tag="PKY09_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNB11555.1"
                     /translation="MQTFEGVVVKTTGNIITVQTGKERILCRIKGSFRIKDINSTNPV
                     AVGDYVKYYFENDDALITELLDRKNYIVRKSKKLSKQVHILAANIDHAYLVATPVLPR
                     TSTGFIDRFLATAEAYSIKASIIFTKADIYDKDILNYVNELKNLYSGIGYSVYVVSSH
                     VSDSLSPLKAQLKDKINLFTGHSGVGKSSLINVLIPGLNLKVTPISEQHLTGKHSTTF
                     AEMHQLPEGGFIIDTPGIREFGTYDFNNNDVTHYFPEFFRLLPNCKFNNCIHVNESGC
                     AVLAALEHGEISPSRYHNYLSILNNEDIFR"
     gene            31638..32096
                     /gene="dtd"
                     /locus_tag="PKY09_01500"
     CDS             31638..32096
                     /gene="dtd"
                     /locus_tag="PKY09_01500"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNB11556.1"
                     /translation="MKVVIQRVTEASVEIEEAIYSSIKNGLLVLLGISVADEKEDVDW
                     LCKRIIGLRIFSDENGLMNLSVKDINGQILLVSQFTLYGDANKGNRPSFIQAARPEKA
                     IPLYEYFIQQLCKNDINVYTGKFGADMKVSLTNDGPVTIIIDSKKHTNDH"
     gene            32086..32412
                     /locus_tag="PKY09_01505"
     CDS             32086..32412
                     /locus_tag="PKY09_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNB11557.1"
                     /translation="MTIKDSQQIVDDWIKKHGVRYFNELTNLALLTEEVGELARIIAR
                     KYGEQSAKNNEDSLQMADEMADILFVLICLANQTGTDLEEAFKNNLIKKTNRDATRHK
                     VNKKLL"
     gene            complement(32404..33141)
                     /locus_tag="PKY09_01510"
     CDS             complement(32404..33141)
                     /locus_tag="PKY09_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11558.1"
                     /translation="MKPDSIYIFIVIAVTFLISGCSPKYYVPPQPNIPALANGGEGKF
                     GTSFSLPQFGVGGMAFDAQMSYSPIKHLGLTADGMYFSGGDKTNDYSGKGIVGNVGLG
                     YYGMAGERWGADVYAGIGKGSIRMNQDSLKFFSSELMRYYIQPGVFYRTRRFDFGLVF
                     RLSRLLYEDMKYYSQADNDDMLYGEKFLFYEPSLYLALGGNLVKAKFQITPSFKIDNE
                     LEFNRQKLVLSFGIDFLIGRNRADKLK"
     gene            33291..34163
                     /locus_tag="PKY09_01515"
     CDS             33291..34163
                     /locus_tag="PKY09_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNB11559.1"
                     /translation="MSNQKAIQTTWISIIGNTLLAGIKWIVGYFGNSYALIADAIEST
                     TDIFSSILVLLGLKYASRPADKNHPYGHGRVEPLITFVVVGILVSAATVIAIESIKNI
                     RTPHQIPETYTLAVLAVIIVSKELFYRIVSKRNEKINSSALKADAWHHRSDAITSLAA
                     FIGIAIAIYLGDGYEAADDWAALVASGFIFYNSYLIFRPALGEIMDEHLYDDVVKEIR
                     EVAGRVEGVIDTEKCYVRKTGINFFVDLHVTVNSNISVKAGHQIAHHLKDALIKHNPL
                     IADVLIHVEPDEHD"
     gene            complement(34188..35054)
                     /locus_tag="PKY09_01520"
     CDS             complement(34188..35054)
                     /locus_tag="PKY09_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNB11560.1"
                     /translation="MRQLKITKSITNRESASFEKYLQEIGKEELITAQMEVELARKIR
                     AGDQRALEKLVRANLRFVVSVAKQYQSQGLSLPDLINEGNLGLIKAAKRFDETRGFKF
                     ISYAVWWIRQSIMQALAEQARIVRLPLNQIGAANKVKKAFSNLEQKFEREPTAEELAN
                     ELEIDTDRIAYTMKMPGRHVSMDAPFVPGEDNTLLDVLTNPDAPYADSTLMAESLHTE
                     INRSLETLPEREKDVIKLFYGIGVEHGLSLEEIGEKFDLTRERVRQLKEKAIKTLRQN
                     SKSKLLKAYLGQ"
     gene            35548..36006
                     /locus_tag="PKY09_01525"
     CDS             35548..36006
                     /locus_tag="PKY09_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11561.1"
                     /translation="MIKTTKIFAVLVCLLVVTSLTAFGQKSKVNQEQKITGPGKCLIK
                     TDSLLTNELTKEDLIKWVENPPFIVNCQDTKTYKLKFYEFTILKMKPFENKSFGVGDD
                     RMIPILGRKAINDLKEGDTVILKGVDLIEDGTDKTAKAPTISIKIIEKQL"
     gene            complement(36003..36989)
                     /locus_tag="PKY09_01530"
     CDS             complement(36003..36989)
                     /locus_tag="PKY09_01530"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNB11562.1"
                     /translation="MKPKKLNSIYRFLPFHFIRKWHVVILLIGIFSNTFCQDSVNTQQ
                     KIPFFEPSPVYNKQRVQIAGYTTLGLYAGTMAGLYQLWYSGYDMNHFHFFNDNGEWLL
                     MDKTGHTFSSYIAGKQGYNVCRWAGMDNKKATLIGGNLGWIFLVSVEALDGFSSAWGF
                     SWGDIAANTLGSSVFIGQQLLWQEQRIEFKFSFHQSKYAHYRKDLLGENYIQQLFKDY
                     NGQTLWLSANINSFIKKPKVIPAWLNIAVGIGADGMTGAFDNATEYKNNPIPNFKRYR
                     QIYIAPDIDLTKIKTKSKALKLFFEVTRFIKFPLPALEINSKGQWLFHPIYF"
     gene            complement(37076..37447)
                     /locus_tag="PKY09_01535"
     CDS             complement(37076..37447)
                     /locus_tag="PKY09_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB11563.1"
                     /translation="MQHDDLHIEKLYYSIGEVADMFKVNTSLLRYWEKEFTMLHPKKN
                     LKGERHYTKKDIEAFRVVYHLVKEKGFTLNGAKQYLKTNGKSIPPSGVKEITEVKEDN
                     SHVKFALNRLKAFLIEMKGNL"
     gene            complement(37478..38449)
                     /locus_tag="PKY09_01540"
     CDS             complement(37478..38449)
                     /locus_tag="PKY09_01540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB11564.1"
                     /translation="MAKAKYYFNTHSLKYEKVVVSFKKRFLRVLGWLATTMVFSAIII
                     ALAYQFLDSPKEKQLKREIAVMDLQYEILQQKLDLMSKVLKDIEYNDDNIYRVIFEAD
                     PIPESVREAGYGGVDKYRSLKGYDNSALMIETTTQLDKLMRQMYVQSKSFDEVFELAK
                     NKKELLASIPGIQPVSNKELSRIASGFGYRIHPIYKTSILHTGIDFSAPVGTEIYATG
                     NGLVKKVEKEGRGYGNNVTINHGFGYETLYAHMSKIVVKPGQKVIRGQIIGYVGNTGS
                     STGPHCHYEVIKNGSKINPINFFFNDLTAAQYEQVLEISSKYNQSFD"
     gene            38583..41201
                     /gene="alaS"
                     /locus_tag="PKY09_01545"
     CDS             38583..41201
                     /gene="alaS"
                     /locus_tag="PKY09_01545"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNB11565.1"
                     /translation="MTSSEIRKTFLDFFHSKQHTIVPSAPIVVKGDPTLMFTNAGMNQ
                     FKDWFLGNQPAKYRRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDPTD
                     PKAGYFKKEAIAWAWELLTEVYKLPKDRLYVTVFEGDKNENLLFDEEASNCWKQHLDH
                     SRILMGNKKDNFWEMGDTGPCGPCSEIHVDLRNEEERKQTDGKLLVNTGHPLVIEIWN
                     NVFMEFNRKADGTLEKLPARHVDTGMGFERLCMAIQQKKSNYDTDIFQPTINYIEAMS
                     GIKYGSNEQTDIAMRVLSDHIRAIAFTIADGQLPSNNKAGYVIRRILRRAVRYSFTYL
                     NFKTPFIHRLTPLLADQFKDVFPELFEQKDFVQKVIKEEEQSFLRTLETGIRRFDQYN
                     KSVIDGEFAFELYDTYGFPIDLTQLIARERNMQVDMNGFNQCLEQQKERSRAASAVDA
                     EDWVILENSESKFIGYDNTTAEVIIIKYRKVKSKNKEQYQLVFNQTPFYAESGGQVGD
                     TGYIQNEKEKIFITDTQKENNQIIHLSDKLPVDLQADFIATVNSSRRRKITANHSATH
                     LMHAALKQVLGNHVVQKGSLVNEEHTRFDFAHFAKVSDEDITNIEQLVNEKIRENIAL
                     DERRNVPIEEAKSMGATALFGEKYGEYVRVIIFDPAYSMELCGGTHVTATGEIGLFKI
                     ISESAVAAGVRRIEAITSIKAEEYINNQLRQLNTIKSELKNPKDIVVRVKELLSENEI
                     LQKQIESYVHEKAQRIKAELLKKVRLQNNINVIAQKIDLANADAIKNISFELRNQLPD
                     SFILLASESDGKAMLSLIISDQLVQSGKLNATTIIRSISKHIQGGGGGQPFYATAGGK
                     NPAGIDAAIEEALKMI"
     gene            complement(41259..42110)
                     /gene="gldN"
                     /locus_tag="PKY09_01550"
     CDS             complement(41259..42110)
                     /gene="gldN"
                     /locus_tag="PKY09_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNB11566.1"
                     /translation="MKKVLLFCFLFAMAFTVKQTNAQTVLDGVYVKEHNPTRKVMEYA
                     FLREADVLWAKRVWEVIDLKQKINLPLRFPREGDTKDRKSLIEVLMNAIDEGSLTAYS
                     TTDDEFTTPMTLEEFKKIGGAGMDTIQITDPEPPYLTRDSAVQRTFSRDKVIAYRIKE
                     EWFFDKQRSVMECRIIGIAPLMLAEDEYGNIREGNIQKPLFWIYYPEARKLLANAEAF
                     NRENDTERRTFDDVFHKRMFNSYIIKESNVYNRRIEDYKTGLDALLEAERIKSEIINL
                     EHDMWEY"
     gene            complement(42141..43745)
                     /gene="gldM"
                     /locus_tag="PKY09_01555"
     CDS             complement(42141..43745)
                     /gene="gldM"
                     /locus_tag="PKY09_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNB11567.1"
                     /translation="MASGKLTPRQKMINMMYLVLTALLALNVSKEIINAFITIDEGIT
                     ASNLNVIKKNQQVMDAFATAAQADAVKAKPFQEKANQVHKASDDLYKYVEDIKNEIVR
                     KADKLEANAKIPGPRDIQKNDDYDTPTLVMCGDKQDGKGFKGTELKNKLEEYKVKLIG
                     FVDADDKKDFQTRFDELINLKDPDPNSKLAKEEKKKTWEMATFYHTPVVAALAMLSKI
                     QSDVKTAESQVNNYLLSQINAKDLKFTDVEPKVVAPTSYVLTGQDYKADVFLAAFNKS
                     SDAEIVVGGRTLPVENGLGKYVDRPSSEGVKKWGGVIKVKDPEDPTKVKEYPFEAEYI
                     AAKPAAVVSPTKMNVFYIGPENPVSISVPGVPNNKVVASISGGGGTLTKNPKGTGADY
                     IVKVTQQGEATISIIADMDGKKINMGAMKFRVKRVPDPVAKIAGKREGPMQKNALIAQ
                     SAVIAELENFDFEIYYKVTKFKMSLYPKGKDPIELESNNNLITANMKAALSKARAGDK
                     VYFEYIKAVGPDGGTRSIPSVNFTIQ"
     gene            complement(43801..44610)
                     /gene="gldL"
                     /locus_tag="PKY09_01560"
     CDS             complement(43801..44610)
                     /gene="gldL"
                     /locus_tag="PKY09_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNB11568.1"
                     /translation="MGLAEFTSGKKYKKFMAMLYGLGASVVIAGALFKIQHWEGAGIM
                     LTVGLLTEAVIFFFSAFEPLHEEIDWTLVYPELAGMHDPNNARDKKKKIDPVSQALDK
                     MLSDAKIGPDLIASLGTGLKSLGDNTAKMADLSDASVATNDYVKNVNAATKSVTELSG
                     SYQKAAQAMSSLSTASDDVKMYNEQVQMAGKNLGALNAVYELQLQDSNNHLKQTSKFY
                     DGINQLMSNLNESLDDTKKYKDEVSKLAKNLSSLNTVYGNMLSAMNLNPNK"
     gene            complement(44685..45947)
                     /locus_tag="PKY09_01565"
     CDS             complement(44685..45947)
                     /locus_tag="PKY09_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNB11569.1"
                     /translation="MRKLFIPIIAVILLSSCGGGNKGQLVGALNRERWYQADPYGMVF
                     IPSGAFNMGPSDQDVPYAMVAQSKSVSVHAFYMDNTEITNNEYRQFVEWVRDSLAHRL
                     IGGDHLIEEGEYGERINWRKKIKWEDEQNVETLAELFLPESERFYRRKEIDSRKLNFE
                     YYWIDLKEAAQKSNREQGMTDRSVFIKKDIINVYPDTLAWIHDYTYSYNDPVTNMYFW
                     HPAYDDYPVVGVTWKQARAFCIWRTQLLNSYLQENGESFVQDFRLPTESEWEYAARGG
                     HDLAPYPWGGPYIRNSRGCFLGNFKPMRGNYMDDGGFYTVKTTSYWPNDYGLYCMSGN
                     VSEWTSNAYEEAAYNFSHDLNPDYSYDAKDSDPVALKRKVIRGGSWKDVGYFLQTGTR
                     SYEYQDTAKCYVGFRCVMSFLGRDKADF"
     gene            complement(46097..46999)
                     /locus_tag="PKY09_01570"
     CDS             complement(46097..46999)
                     /locus_tag="PKY09_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNB11570.1"
                     /translation="MKRLFTVVAVATASVSFGQQDPQFSQNMFNRLYPNPASAGTNEA
                     ICGTLIYRDQWDKFGGGPKTAVFGVESPVEVLHGGLGLSVLTDKPLNQKNLNIKLAYS
                     YHLDLGSGKLGIGLDAGLLQRGYGNDFNAIDPDDPLVKSGTGSKFDLGAGLYYKSDKF
                     YVGASATHLTGGKIEYTDDAKVEVVPHFYFMGGVDFDLTPSLKLKPSLFIKSDAKETQ
                     ADINANLHIKDKVWIGASYRLQDAIVLMAGINIIENLKLGYSYDITTSKLKGYHSGTH
                     EIMLGYCYVIKPKVIPVLRNVRFL"
     gene            complement(47128..48300)
                     /locus_tag="PKY09_01575"
     CDS             complement(47128..48300)
                     /locus_tag="PKY09_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNB11571.1"
                     /translation="MQISEFFSPISESVEQKMSNAPVDSMWHHIEKNLGSSGFPFMNN
                     IKIAIVGVEETRGAKRDYEAKNGVDYVRQSLYSLKKHGVGVPVADLGNVRLGVTVEDT
                     YAALTEIVISLLQLKIIPVIIGASQDMTVAHYRAYKKIDQIINMVGVDARFDLGMPSD
                     PLDSASWLGNIVMDQPNHLYNYSNIAHQTYFVGDYAVNMMDNLYFDTCRLGEVNQDAT
                     EMEPIIRNADVMTFDLSSIRYSDASATSTPSPNGLTGDMACQIMYYAGMSDKLEGIGI
                     YEYAPSADTNQMTAALIAQMVWYFMEGVSNRKNDFPDSTSSQFTVYNLTLRENNEEMH
                     FLKSNVTGRWWMEIPLSKIKKKLDRHQLVPCSYEDYQKACNNEIPERWWQALKKLS"
     gene            complement(48303..50906)
                     /gene="topA"
                     /locus_tag="PKY09_01580"
     CDS             complement(48303..50906)
                     /gene="topA"
                     /locus_tag="PKY09_01580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNB11572.1"
                     /translation="MAENLVIVESPAKAKTIEGFLGKDFVVKSSYGHVRDLAKANNGI
                     DIKNNFEPIYEVSEDKTKVIDELTKLSRKAKTVWLATDEDREGEAISWHLSEVLNLKD
                     AATKRIVFHEITKDAILEAIQNPRKIDIHLVDAQQARRILDRLVGFELSPILWKKVKP
                     SLSAGRVQSVAVRLIVEREREIQDFEVASFYKVVANFLVKDKSGKTTILKAELDKKFG
                     TEKEAEAFLEKNRNATFNIASLEKKPSARKPAAPFTTSTLQQEASRKLGYAVATTMSI
                     AQKLYESGMITYMRTDSVNLSDQALANAEKEINTSYGKNYHQKRKYQTKSASAQEAHE
                     AIRPTSFNRSVIKGTPGEQRLYDLIWKRAIASQMADAKLERTIVKIASDKSTEKFVAT
                     GEVIIFDGFLKVYTESSDDENEEDNDTLLPPLKEGQKLDFKSITATQRFSQPPARFTE
                     ASLVKKLEELGIGRPSTYAPTISTIQKRGYVIKEERQGKERKYAVLELSNSKINKVEK
                     TEITGAEKNKMYPTDTGIVVNDFLVEYFKDILDYNFTAEIEKEFDEIAEGKLKWNKMI
                     AGFYKPFHKNVENTIETSDRATGERTLGKDPKTKRKVIVRLGRFGPMAEIVAEKESDT
                     PMYASLQKNQRLETVTLEEVLDLFKLPRHFGQYKNKELIVNNGRYGPYIKFGEEYISL
                     PKEEDPFTIELDSIKAILDGPRLPRTLGQHKGLDIVINKGRFGPYIKYGVEFISLPKG
                     EDPLEIDLETVVDILNKPRLPRNAGQYKGKDVVIAKGRFGPYIKYDDLFVALKKGDDP
                     FDVNIDYLGELIEAKLSGVPLKKEKAAKAEKSSVAKKTKAKSPAKKSSASTKKKAASS
                     GKTKSKAKSGR"
     gene            51105..52577
                     /gene="miaB"
                     /locus_tag="PKY09_01585"
     CDS             51105..52577
                     /gene="miaB"
                     /locus_tag="PKY09_01585"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNB11573.1"
                     /translation="MHNDIKVIDESRQGEPTMLQSSSENLSPKKLYLESYGCQMNFAD
                     SEIVASILKNEGFETTKDVNEADAVFINTCAIRDNAEQRVRTRLKDFKKIKKENPDLI
                     VGVLGCMAERIKSQFLEEEKLVDIVVGPDAYRDLPLLIEKVEDGQKAVNVILSKEETY
                     ADITPVRLGNNGVTAFVSIMRGCDNMCSFCVVPFTRGRERSRDPQSIIKEVEQLYNEG
                     YREVTLLGQNVDSYLWKGGGLKKDLDTETVHLALNHKLPPEQQKDFVTFSNLLEMVAD
                     VNPDMRVRFSTSNPRDMTDAVLHSMAKYENICKYIHLPVQSGSSSVLEKMNRGYTRED
                     YIARIESIKRILPECGISTDIIAGFCSETEEEHQETISLMEQTIYDFAFMFVYSERPG
                     TFAERKFTDDVLPEVKTKRLNEIVALQQKHSAIRTNQSIGKVYKVLIEGVSKKSDEML
                     MGRNSQNTVVVFPKNNHKPGEYVHVKVNKCTSTTLIGEVV"
     gene            52660..53880
                     /locus_tag="PKY09_01590"
     CDS             52660..53880
                     /locus_tag="PKY09_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB11574.1"
                     /translation="MTIQDIKARFGIIGFSSVLDHALNTARQVAPTDLTVLITGESGS
                     GKEVFPQIIHQLSSRKHGPYIAVNCGAMPEGTIDSELFGHEKGAFTGAHEMRKGYFEV
                     SNGGTIFLDEVAELPMFTQVRLLRVLESGEFLKVGSSKVQKTDVRVVAATNVNIPEAI
                     SRGRFREDLYYRLNTVPIHVPALRERTEDVHLLFRKFAGDFAEKYRMPALTLQPEALR
                     LLMEYRWPGNVRQLKNVTEQLSIIEQKKEISAEVLLKYLPQENFASVPTLFNNHKGGD
                     LNERELLYKVLFEMKKDISELKKIVFNIISNGEPVHLENSDIDTERFLNDVKGHPVLH
                     TNDTSITLTKPTHIDVKPHEHVEESLSIADREKDLIIRALEKYRGKRKGAAKELGISE
                     RTLYRKINEYGIKD"
     gene            53946..54422
                     /locus_tag="PKY09_01595"
     CDS             53946..54422
                     /locus_tag="PKY09_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNB11575.1"
                     /translation="MAFTAMMESCGVYSFTGASITPGVKTVSIQYFPNRASVAQPSLS
                     QVFTDKLKEKFVSQTSLIQVNQSGDLRFEGYISDYNVQPTAIQSTDVAALNRLTITVH
                     VKFENVKDEKQNFETSFSRYADFDSRKDLTSVESSLMNDISSQLVDDIFNKSVVNW"
     gene            54422..55846
                     /locus_tag="PKY09_01600"
     CDS             54422..55846
                     /locus_tag="PKY09_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11576.1"
                     /translation="MTKERFFNFIQQPYNIQTNDIEELEQIIEQFPYFQTARLIYTKS
                     LHNENSYLYNDELKRTAAFAADRSVLYKLIHTINKDTHIVDSDSLDSDEIASRVEISP
                     VQETPEVDALVFEEKTEAETQVIIFEEKDKSDADDEPILFEEKTLANEEVEPVVFEEK
                     LPETTATEAIIFTAIEEPINTNITEVDENEPSNKNAVIASEKKERALTPAEILSQRLR
                     EIESQLEDKKEEETEAVSELKEEKSLSEPPKITEPVNIQPEVLPESIEINPSLLEIKE
                     EVEIPEPEEVENLIAPAPENTEKTVEVVVASPEIKSSIIEDNVTPDAIIQPEIKPLPE
                     VDKKEKHSFSDWLHLFQPQEEKKTLITNALVESNSDKNLISNKLTGEDIAKNINSEII
                     NQFIQNDPRIESSKTKFFSPGNLAKSSVTDVSDIVSETLAKIYLGQNNFSKAIQTYEK
                     LILKYPEKSVYFAALIKEIKKSQI"
     gene            55859..56224
                     /gene="secG"
                     /locus_tag="PKY09_01605"
     CDS             55859..56224
                     /gene="secG"
                     /locus_tag="PKY09_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNB11577.1"
                     /translation="MYTLITVLIILISVLLCLAVLVQNSKGGGLASGFSAPQQIMGVR
                     KTTDFLEKFTWGLGTALIVFCVIANFMTPGSNNSTEVRASSIQEQIDNATLPASPQAP
                     LPSQQNAQPAQQPATQPAK"
     gene            56409..56687
                     /locus_tag="PKY09_01610"
     CDS             56409..56687
                     /locus_tag="PKY09_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNB11578.1"
                     /translation="MSKINLTPLADRVIVEVAAAEEKTASGIIIPDTAKEKPQKGKVV
                     AVGGGKKDEPMTVKVGDSVLYGKYAGTEISVDGKEYLIMRESDIFAIV"
     gene            56756..58387
                     /gene="groL"
                     /locus_tag="PKY09_01615"
     CDS             56756..58387
                     /gene="groL"
                     /locus_tag="PKY09_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611460.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNB11579.1"
                     /translation="MAKEITFNLDARDALKKGVDALANAVKVTLGPKGRNVIIDKKFG
                     SPTITKDGVTVAKEIELTNSIENMGAQMLKEVASKTADIAGDGTTTATVLAQSIVNTG
                     LKNVTAGANPMDLKRGIDKAVEAVVASLKKMSKEVGDDNKKIEQVATISANNDSEIGK
                     LIAEAMAKVKREGVITVEEAKGTETTVEIVEGMQFDRGYISPYFVTNADKMQAVLESP
                     YILLYDKKVSNMKELLPILEKVVQTGKPLLIIAEDVDGEALATLVVNKIRGSLKIAAV
                     KSPGFGDRRKAMMEDIAILTGGTLIAEEQGFKLENADLSYLGTCEKITIDKDNTTIVG
                     GAGKKADITARVNQIKAQIETTTSDYDKEKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALHATRAAVEEGIVAGGGVAYIRAITALDKLKGDNDDETTGIAIIKRSLEEP
                     LRQIVSNAGIEGSIVVQKVKEGKDDFGYNARSDKYENMHAAGVIDPTKVTRVALENAA
                     SVAGMLLTTECVLADIKEDKPAMPAMPGGMGGMDY"
     gene            58505..59419
                     /locus_tag="PKY09_01620"
     CDS             58505..59419
                     /locus_tag="PKY09_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11580.1"
                     /translation="MKKSLLVLISCLFVNTNIHAQSNTTNIDEINANLKSNEIVYTQK
                     YQAIFNQPKITSVLTGCDSITTTYSSNNGLAGAMFDVIADSNLVINGIYTNLSVASGV
                     FEIYYKYGSRVLYLQNPTAWTLLGSASVVSAGIDVPTFIPIAMNITMNAGDTVAFYVT
                     NTNGGSSGLKYTNGVGSGTLYTDNGALKIFEGNGITYPFNSLFADRVWNGTINYCTNT
                     TGVTNLKNDNITIYPNPASDYIKFIFENNNLKTLIIKSLDGKKMFEAININTSDYIFK
                     RNGLAKGIYFYQIYSNDIEVAKGKLILN"
     gene            complement(59965..61656)
                     /locus_tag="PKY09_01625"
     CDS             complement(59965..61656)
                     /locus_tag="PKY09_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNB11581.1"
                     /translation="MKNLILTGLTLGLTCMMSYAQKYDEMKTTWEIEATHPFSETGLS
                     EEKAIVHGSNEKMFTMVSTVDGKVKWSKKFKEIDEQIGKVDLQIPMYESNAVFLFDKK
                     AGKDQMVVVDMETGVKLWSTNKYQGIDDPDEVIYIAELNAYAIVTKTNLTMVKARTGE
                     ELWSTVKFSTPVGKYFYDEKENAIVIISMPRNAWQSMFKGFRNQILKINAKNGDILWE
                     QTFVGIVERKIVTRESVAKIFLRGDKLFLQLNGLQVYEYKTGSPIWAAAYDVTFAREL
                     VKPKVYGQVVRYGVYEAIAQPLYDGEYVYILDVKSRKSQYLKKYEINSGKLVWTSPEI
                     KDARVIPGLYKIEDKIILQIGGAVEVQAISKQTYGNGYVQWTQYFRTIKYQNVKPYEV
                     QAFSTTDGTKSWDSERFRKGITNIFPSGNNIVVSSGKALYSLDYKTGKEAYEIPLGDD
                     DIGLAEKIVNPSELGDNISKDNVIVLGEKGVSSHNIATGAKVWAVKTKKGEFSGIFGE
                     NIFFDTEKGDYFSVDANTGKAKFFDSRKNSKSIVSLDGKSIYVFEGKKILKVSTE"
     gene            complement(61698..>62088)
                     /locus_tag="PKY09_01630"
     CDS             complement(61698..>62088)
                     /locus_tag="PKY09_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11582.1"
                     /translation="PVAYLSNGTKLIEWNYETLKEYTDYIEGQDNDSLQSKAISQLNT
                     DLENPESDLAKNIIKQSIKGIYLFDLTIQKKGVIVTIFAESDDKTDIPKQNALKDLLL
                     KYKFKDIVVSPNQRLKFRYTFNIDNSI"
BASE COUNT        19831 a        11382 c        11199 g        19676 t
ORIGIN      
        1 aactaagttt tgtcctctga taaattgcga caaaaataat aagctgaaaa atataaaaga
       61 tttcatactg taaagataat attgtccttt caaagtcccc tgaaatgcga tagactttga
      121 aagtaaggag aaaggagaaa aatagcaaaa aagagattgc gatagttgtg tagcagatgc
      181 ttataaaagt acgctgtaaa ataaattata tcgtttctaa cgtgtataat ttggtgcttc
      241 tttaacaata gtcactccat gtgggtggct ttctcgtacg ccactttgtg taatacggat
      301 aaattttgct ttctgtaagt cagcaatatt cttggcacca cagtagccca tacctgccct
      361 tagacctcct actaattgat aaaccgtttc agcaagtgtt cccttgtatg gcacgcggcc
      421 aacaatgcct tccggcacca attttttaat atcatcttct gcatcctgaa aataacgatc
      481 ctttgaccct tcgcgcatag cttcaatgga gcccatgcca cgataagatt taaattttct
      541 tccttcaaaa atgatggttt caccgggtga ttcttcaaca cctgcaaaca tagaaccggc
      601 cataatgcta tttgcacctg ctgcaattgc tttaggaata tcaccggtaa aacgaatacc
      661 accatctgca ataatcggta cgcctgaacc tttaatggct gaggcagcat cagcaacggc
      721 tgttagctga ggcactccta cacctgctac aacacgagtt gtgcaaatag aaccggggcc
      781 tattcccact ttaacaccat cagctccatt tttaaccaat agcttggctc cttcggcagt
      841 accaacattt cctacaataa catctaatcc tgaaaatttt ttctttacag actttaatgc
      901 agtgataacg cctttggaat gtccatgggc tgtatcaata actatggcat caacacctgc
      961 atttaccaac gaggaaacac gatcaagcat atctgctgta acaccaatgc ctgctgcaac
     1021 tctcaatcgc cccagcttgt ctttgcatga attgggacgt gatttaaatt ttatgatgtc
     1081 tttgtaagta actaatccta ttaactttcc tgaacggtca acaacaggca acttctctat
     1141 tttatactga cgcaaaatca cttcagcttt ttgcagtgaa actactcctg taacagttat
     1201 cagctgtcct gatgtcatga cttcttttac cttacggctt tcgtccgttt cgaacctaag
     1261 gtcgcggttg gtcaaaatgc ctactaactt atttttgtca ttaacaatgg ggataccacc
     1321 aatgcgttgt tcgcgcatga tgccaagtgc ttcgtgtatg gttgcgtttt gatgtaacgt
     1381 aatcgggtca tgaatcatac cactttcaga cctttttact ttccttactt cattggtttg
     1441 ttgctcaatg gtcatgttct tatgaagtac accaatgccg ccttcctgtg caatagcaat
     1501 ggccattgcc gattcagtaa ccgtatccat tgctgctgaa acaatgggaa cattgagctt
     1561 tatgtttctt gagaaataag ttgatgtatc aacatcgcgg ggtagtatct ctgagaaagc
     1621 aggaacgagc aaaacgtcat cgtatgttaa tccttcctgt aagtttgatt ttgatgcaag
     1681 agccatggta tttgcttttg ccgggcaaag atacagaata aaaccgcttg agtggcgtaa
     1741 attaaaagtt aatttctaac ctgaagttct cccttttcaa tggactgaac tactttctca
     1801 atcattttat ctgcgttatc agggtcgtac tccgacttga ggtttgcact gaaaatttta
     1861 gctatcccct gccagaaaaa cgcctgaaaa gagccacgta aatctttaac cagattatat
     1921 gaggtgtgcc ctgtttcacg atcaatcagt ttgatgagta ttttaccttg cgtataggtc
     1981 agatttttta aatctttctc aaactgctct ttcataattt tttcctgctc cttaatgaat
     2041 ttttttttct ccttttctga agtcattttg gcaacagact cttcaataaa cctaagttct
     2101 gccgaagcaa tttttgcata cggatatgcc ttcaaaacat ctctgcgcaa cttcatgtat
     2161 ttacgtacat catcagatgc atttgccgga ataaagccgt aaatatctac agcatttaaa
     2221 gtgtaataag caatagtgtc accattataa actgctgttg acgagcccac aggtgtttgc
     2281 ccattgctga atttgataaa agcagtgaac aagaaacaaa gccatactga aaacttcatg
     2341 actgattttt tagcaaagac actgccgatt tcacaattta ttattttctt aatcattagt
     2401 ttaaccaaac gtaggttcag gatcggttgt tgcgccagta agaaatagtt ttctcctgtt
     2461 gttgttgaat agcagcaggc ttcttttctt caagccatag ttgtgctgcc aatgacgcta
     2521 tttctgcttc gtctgcaatt gttttttcaa gtacttcagg cgtgaaatac ttttttacaa
     2581 aatgcgtatc aaactttgca gtaacaaaag cattatgttg catcacaaag cggcaaaatg
     2641 gcaaggttgt ttcacagcct gtaattttat attcatcaat tgctcttatc attcgtgcaa
     2701 ttgcctcatc tctgtcacgt ccataggtaa tcaatttggc aatcatcggg tcataataaa
     2761 taggaatttc catgccttgt tcatagccat catcaactct tacacctaca ccccttggtg
     2821 taatgtaggt agagagttta ccaacgctgg gaagaaaatt gttggcaggg tcttcggcat
     2881 aaaccctgac ttcaacagca tggccatgaa tttttaaatc ttcctgactg aaacttaatt
     2941 tatttccggc agcaacatgc aattgctctc tcaccaaatc aattcctgtt atcatttccg
     3001 ttaccggatg ctctacctgt aaacgggtat tcatttcaag aaagtaaaaa ttattcattt
     3061 catcaagtaa aaactctacc gtacctgctc ctgaatagtc acatgccttt gcaaccatca
     3121 ctgcacattg ccccatggct gctcttgtct tttcgtctaa aactcccgaa ggcgcttctt
     3181 caattacttt ctgatgcctg cgctgaacag aacattctct ttcaaaaaga tggacaacat
     3241 taccatgatt atcgcccata acctgaattt ctatgtgccg gggcgaagca atatattttt
     3301 caataaaaac acttccgtca ccaaaggcag attgcgcttc gctcatggcg gattttactg
     3361 attcattaaa ggctttttcg ctctctacaa tacgcattcc ttttccacca ccgccagcac
     3421 tggctttaat caataccggg aaaccaatat caacagcaat ttgtctggct ttttcgatat
     3481 cactaatggc gtagtctgtt ccgggaacta atggcacatt aaacttctta gccgcttctt
     3541 ttgctcctaa tttactgccc attgtccgca tggattgtgg tgaaggtccg atgaatacca
     3601 aaccggcatt aataattgct tgggcagcat ctgcattttc tgataagaaa ccatatcccg
     3661 ggtgaatggc ttctgctcct gtcagttttg ctgcttgaat aattttttca accaacaagt
     3721 aagattgcgc tgatgatgag gcacctatgt gtacagcttc atcagcacaa agaacatgtg
     3781 gcgccatacg gtcggcatcg ctgtaaacag caacagtttt aattcccata tcacatgcag
     3841 tacgcatgac tctcaatgca atttcgcctc tgttggcaat caatattttt ttaaacatga
     3901 agttgctttt tactttatgc tgcaaaggta aaacgaactg tgaatcaaac cttttacaaa
     3961 tgctaaattt attttaaaat acaggttaat aagttctgta cagaaaagtg ggtgtatgtt
     4021 atcttgaatt tctttatttt ttgttacttt gcacctcata aaaacgatga aatattatga
     4081 atgaatacgg attaagaaac ccaaactttt ctgttgctga acttggctta aagaatgtag
     4141 cagcagccta ttggaatctg acagttgcag aacttgttga agaagttgtt atcagaggtg
     4201 aaggtgaact tacagacttt ggtgcgcttg cagttgatac tggcgagttt accggacgtt
     4261 ctccaaaaga caagtttatt gtagccgaca aggaaacaga aaattctgtt tggtggggag
     4321 atatcaatat taagtttgat gctgataaat ttgatgctct ttacaacaga gttactgctt
     4381 acttatcagg taaagaaatt tatgtccgtg attgctatgc ttgtgccgat ccgcgatacc
     4441 gtttaaatat ccgcgtaatt actgaatcgc cctatcaaag catattctct aacaacctgt
     4501 ttttaagacc tactccggaa gagattacta ccatggaaaa agattgggta atcatggcag
     4561 catcaggttt catggccgac cctaaaatag atggtacacg tcagcataat tttgctattg
     4621 ttaatttcac aaaaaagata attcttatag gtggaacagg ttatacaggt gaaattaaaa
     4681 aaggtatttt ctctgtattg aattacattc tgccacatca taagaatgta cttagcatgc
     4741 actgctctgc caacattggt aaaaacaacg atactgcaat cttcttcggc ctttcaggta
     4801 caggaaaaac cacacttagt gccgatccaa acagaggctt aattggtgat gatgagcatg
     4861 gatggagcga caatacggta ttcaattttg aaggtggctg ctatgctaaa tgtgttaacc
     4921 tgagcaaaga aaaagagcca cagatatatc aggctattcg ctttggtgca ctcttagaaa
     4981 acaccgaatt ctttgaagga acaacccgtg ttgactttga taatatttca aaaacagaaa
     5041 acactcgtgt agcctaccca atacatttta ttgataatgc tgttgagcct tcagtggcag
     5101 gtattccaaa gaacatcttc tttttaactt gtgatgcctt tggggtgtta cctcctattt
     5161 caagacttac aacaggtcag gcaatgtatc atttcattag tggatatact gcaaaagttg
     5221 caggaacaga agttggtatt accgagccaa caacaacatt ctctgcctgt tttgggaaag
     5281 catttctacc cctgcaccca acaaaatatg ctgaattact tggcaaaaaa ctaagtgaaa
     5341 ataagaacat tcgtgtttgg ttagtgaata cgggatggag cggtggttct tatggtgttg
     5401 gttcaagaat taaacttgcc tatacacgtg ccatcattac tgctgcatta aatggtgagt
     5461 tagataatgt taagttcgaa accttaccgg tgtttggatt gcaatatcct gtttcatgtc
     5521 ctaatgtgcc tgcagaaatt ttaaatccta aagctacatg ggctgataaa attgcgtttg
     5581 acaataaatc agctgatttg gctgccaagt ttgtgaagaa tttcgagcag tatgcttcac
     5641 atgctaataa agaaattatg gatgcagcac caaaggttgc tgttgtagct taattttttt
     5701 aatttttttt ctcccccggg ttttctcggg ggattttttt ttgacttata ctgaattgat
     5761 ttttgcctaa agaaactttc cttttaccaa atagtgggta tttgtcttgt tgctgatatt
     5821 actttagagg catgttatta ctatttacat tactcaacat tacagccatc gttttctttt
     5881 tctgtattaa ggtgcgttca aaaagcaacc gccaccattt ccattttcat gtcagcaaat
     5941 aatttgctga ggcagttttc agacagtaca gataagctta tccccttctc ggaaggaaat
     6001 tattcctttt agagtcattg ggtttttgat ttttgcaaat tgtcatcata tttgacctgt
     6061 taaaataaaa acacaggtta tgaagaaact ctttacacta attttcattg caatagcagg
     6121 tcttgttgtt gcagatattt tgaatgaaca aggacaagct gttgccacag gatcacccgg
     6181 tgaatttgat tgtacatcat gccataccgg caatagtgta aatagtggta ccggttccat
     6241 tacaatttca tcacccaata tgcccaactg gcaatatgtt cccggtcagg tttatcaaat
     6301 tgatgtaaca gtagcgcaaa ctggtgctcc gctgtttggg tttgggtttg aagcactcag
     6361 aacaagcaat aatactaacg gaggcacttt tcagattacc aattctgcaa gcacttttct
     6421 taaaacaggt tttataaatg gctcgaacag aactaatgtt gtccataaaa agaatggcgg
     6481 actctctaat gacttgcata ccttttcatt taactggaca gctcctgcaa ctaacatcgg
     6541 aaatattatt ttctattcag ccggaaatgc cgcaaacaat cagggagata ctttgggcga
     6601 ttttatttac ataacacaac aacttattac gccttctatt acaggattaa atgaaattaa
     6661 gacacctgcc atttctgttt atcctaatcc ggcaacagat tttatcaatg taacattacc
     6721 ggaaagtcaa actgaaacat cgtactcaat taaagattta tcaggcaaaa ttgttgacca
     6781 aggaaagact tttaatccac ttggaaaaag cttttctgta aatttatcag aaacatttaa
     6841 agagggaatt tattttattg aagtcaataa tggcgttgac cattacattc agaaatttgt
     6901 taagctcaag aattaataaa cgttatatag accaaaaaag cagatcaata ctttgcaaat
     6961 ttaagaatct gattttctaa tttaacataa tatattccat ttgaaaacga ggagatattt
     7021 acctcttgct ttgttgaaga acagtgattg gacataatca ttttaccaag catatcataa
     7081 atgtaaaaag gtttgttgct atctgagtta gttgagatat aaattttatc agaggcagga
     7141 tttgggaaaa caaaaatgct ttgcaggttc acttccgcta atgatgtacc tgttccgaat
     7201 attttctgaa agttatctgc atattgataa gatgatgatg aacaattgtt gacagcatcc
     7261 caattgattg accaggtcat cataccacgc aaagttggat agcctccaga ttgaactaag
     7321 gtgtacgtgc ccggctttgg tcctttacca agtaaataat caagtgctgc tttaaccgaa
     7381 gcagtatcag tataaccacc tcctgcagca tttgtgcatg ccggtaatcc tactgccact
     7441 ttattagcag gcaagccggc aaacatccct cctgaagtat taaacccgtg aattactgcc
     7501 tctgtcatgg caacaataaa gtcggcagtg ccttgagtat aaatgttacc atcaatgcca
     7561 aacatggttc cgctgttata aagctgaaca tgcaatattt ctaacgaatc gcgcaaagca
     7621 tcaatgacag gcaaataggc accccaaata ctaccgaatg ccgacatacc cccttgtaca
     7681 aatgcagttt caggtgccat ggttagcatc attttttttc catacgttgc ataatagtta
     7741 ttcatgattt gttttactgc atctatcagg ttgataattt tagcatcaac aggacttgca
     7801 attgttccac cacttacaga aacagaactg ccttcaaagt caatatctat tccatcaaag
     7861 ccatagaagt taattatact tgtcatactc gaaacaaatg aatcacgctc aacagtatta
     7921 tctaaagata ccggtgcagt ggcgccaccc atactgataa ttacctttcg tccctgattc
     7981 tgcaaattct gaacctgcat ggcaaaggct gcaggagaaa cctgctcagg aataaattcc
     8041 atttgatagt cggtgcctgc ttgtggtact gcaaaggcaa cgtcaataat attataacgt
     8101 gaatcaacac tatcaagcat aatgtatggt gccgatgtaa tatcccagtt atgccaataa
     8161 ccaaccaagg caggtgactg cgacaatgcc caaaccggaa gtaataagat tgctgataaa
     8221 attgtaagta gttttttcat cattaaaatt tttaattaat tcaacgctat agggtgcgaa
     8281 acaataaatc tatatttcta tttatcactt taaatcaggt tcaagttttt caagatggtt
     8341 caatgcattc tttagccggc tctctcctat tccattgaca atttgataat ctaatccaaa
     8401 agtatttaat tcttgtaagt agcgttcaaa cagttctttt ctgaagtgcg gatgttctct
     8461 taatggatca tcttcccagg gaatatcaat atcagtaagc aagtgcattg aatagtcgct
     8521 cttctgtatt gcctctacaa tccatttttc tgtacgccca tatttaaagt cgctccaaac
     8581 ttttatcaca actaaattgg tgtcgcaaaa caaaaagtgt tttgcagatt ctgacatgct
     8641 cttttctttt tctaactgca atttagctat tacagacaaa tcatcaaatg tataaggacg
     8701 gtttaattta tgtaagtagt cacgggcaat ttcaggcacc catacagttt gatattgata
     8761 tgccaactgc tcacttaata aactctttcc tgttgattca ggtcctacta tggcaatcct
     8821 atgaattttc ctcatgtctg atgagatggt tctgccattt aaaatagcca ataattgcca
     8881 tgataagata aatggaaaac aaaattgcat acaggggcag gcctttataa agataaattc
     8941 ctatgtataa aatatttgca tcaatccaaa caagccagtt ttcaattttc ttttctgcca
     9001 ttaagtactg tgctgcaaaa cacatgcttg ttgccagtgc atcccaataa ggcaaatgcg
     9061 cgtcagtata gtgactgaac cagctgccca tgccaacagt cagtaaaatg ataccaccaa
     9121 gaagaataaa tataagtctt ttggaagcgt tggtaacagg catttctgtg ttttggctac
     9181 cggtaatcca gcgataccat ccataagaaa gcaataaaat ataaacacct tgctgaacaa
     9241 catcactgta taatgactga tgataaaaaa tataaagtga tacaattaca ttaactaaac
     9301 taatcggaaa gtatgcaatc ttttctttta ttcctaaata aataccaact acgccaaaca
     9361 gaaagcctat ccattcaatc aaaggaatat ttaaaaacag aatagtttgt aagtccacag
     9421 gctacaatgc gtttaaggtt tggtttatag atgattttgc tttggcaatt tcaagcataa
     9481 gttcgggatt gtcttcaatg gcattgccta ccacaataat gtcagcgcct gaattataaa
     9541 tttcaattgc tttttcggca gtacgaatgc caccacctac tatcaaaggc aggcctgtat
     9601 tttttttaac cttttcaatc atctgaccgg aaacagttct tctggcacca ctaccggctt
     9661 caagatatat tacctgcatg cctaaaagtt gtccggcaac tgcagttgaa actgcaagtt
     9721 cattatgatc gtgaggaata ggttttgtat tgctcacata ctgcactgtt gttacattac
     9781 cactgtcaat aagaatatat cctgttggta ttgcttcaag tcctgataac tttacagaca
     9841 tggcacttag aacatgttgg ccaatcagca aatcagcatt acgacctgat attaatgaca
     9901 gatacaatat agcatcagcc tgcgggtcaa cttgtgaggc attgcccggg aaaagaatga
     9961 caggcaagga tgcaaatttt ttcagtatgg caatagtttc aggaatgctt ccatgagtta
    10021 aaatacttcc accaacaaaa aaataatcaa cctgatgcgc tgttgcatag gtgcaaagta
    10081 ctttcaactg cttttcattg accttatccg ggtcaatcaa aacagcaagc tgttttttat
    10141 ggttttggaa ttgctgaagt attttttgct tcatatttat ttttcgatgg caaaagtagt
    10201 tagcaattgg tcttgaaacg aatattgaat atcatagctg caaatatcat tatttaaaat
    10261 taactgacct ttaattttgc cctcctgcag atactcaaat ggtaaaatgc gcaggttaac
    10321 tttaaaatcg agtgatttct taccataaat tttataaata acttctttgg cactccaaag
    10381 catcaatgtt ttttgcagag gattgtcgtg taaaaaatgt tcttcatcgt tgcgaacaaa
    10441 tttatgtgaa acagaagcaa caggcctatt cattaattcc atgtcaaaac cacaattgtt
    10501 gaaacggtga aacaacactc ctacaaaacc atgtgtgtgt gaaatagaaa tgaaaccctc
    10561 gggtgatgcc agaaaaggtt ttccatattc attataaaaa atatcaaagt gtttgtcagg
    10621 cactatctca tgcagaatca acctgactgc gtaccattct gcaattcgtc tttgatgggt
    10681 ataatgtccg ggcaaattat attttccata gaaacgcagc atttgtgaaa gcatatcatt
    10741 gctttctgtc aactgccata ttcctgtaaa actattatca aatttagttg acttaatgag
    10801 aggcataaaa tattcctttg ttagtaggca gcaaatgtgt aaatttgcac aaaattttga
    10861 aaatcataca aattaaatca aaaatatgtc aacagtaaca gattcaaaac tgaagtacaa
    10921 agtaaaagac atttcacaag ctgagtgggg tcgtaaagaa attaaacttg ctgaggcaga
    10981 gatgccggga ctcatgtcat tgcgtgcaga attcggaaag caaaaaccgc ttaaaggtgc
    11041 aagaattgca ggatgtcttc acatgaccat tcaaacagct gtattaattg aaacattaaa
    11101 ggaattaggt gcagaagtta cctggagttc atgtaatatt ttttctacgc aagaccatgc
    11161 agcagcggca attgcagcag caggaattcc tgtttatgcc tggaaaggta tgaacaatga
    11221 agagttcgac tggtgtatag agcaaacact ttttgccttt gaaggtggca agccactgaa
    11281 catgatttta gatgatggcg gtgaccttac caacatggtg tttgacaagt ttcctgaact
    11341 gataaagggc attaaaggat taagcgaaga aactactaca ggcgttcacc gcttgtatga
    11401 acgtatgcaa aaaggcacct tgccgattcc agccatcaac atcaacgact ctgttaccaa
    11461 atcaaaattt gataacaaat acggttgtcg cgaatcgctg caggatgcaa ttcgcagagc
    11521 tactgacctg atgcttgccg gaaaagttgc agtagttgca ggttatggtg atgttggaaa
    11581 aggttcggca gaatcattaa gcagtgccgg tgtacgtgta attgttactg agattgatcc
    11641 aatttgtgca ctacaggcag ctatggaagg ctatgaagta aaaactatgg acaatgccat
    11701 tccggaagca gatattatag tgactgccac tggtaatcac aacatcattg ttgacaagca
    11761 ttttaaagca atgaaacata atgccgttgt ttgtaacatt ggtcacttcg ataatgaaat
    11821 agacatggca tggctgaata aaaattatgg tcatactaaa gacactatca aaccacaggt
    11881 tgacaaatat tcccttgatg gcaaagacat tattgtattg gcagaaggcc gtttggttaa
    11941 tcttggctgt gcaacaggtc acccatcgtt tgtgatgagt aattctttca ccaatcaaac
    12001 gctggcacaa ttagaactct ggaatcatac cgataaatat gaaaacaagg tttacacact
    12061 tccaaagcat cttgatgaga aagttgctcg tctgcacctt aataaaattg gtgttgagtt
    12121 ggaaacttta actcaaagtc aggcagatta tattggcgtt aaagttgaag gtccctttaa
    12181 accggattat tatcgttact aaaagttatt tcacttaatt ttaagaggct gctttatgtt
    12241 aaggcagcct ttttaatctc aggtgactgt gaagtaacta ctatcctcac gcaaatcgta
    12301 acttgtccca ttttaaaata aatctacagg acaaaccact acttttgaca cgtccaacac
    12361 tgttcaattg aaaatactca ttcaatatct caaacgtcac tggaaattag ttctgctggc
    12421 tctacttctt gctgccgtaa accaatgttt ttctttgttg gatcctttaa ttgccggaaa
    12481 aatgctggac cgttttgcca tgcatgtttc cgattacaat gatgatttta ctaatttttt
    12541 taatgccatt atctggtttc tattagcatc aatgggtgtt gctttagtat cgcgcattgc
    12601 aaagaactta caagattatt ttgtcaacgt cattgtacaa cgcaccggtg ccgaaatgta
    12661 tagtgatggc attaaacatt cattagaatt accctatcag gtttttgaag atcagcgtag
    12721 cggtgaaacc ttgggcaaat tgcaaaaagt gcgtgccgac agtgaaaaat ttatcaatgc
    12781 ctttatcagc attctttttc aaagtgtggt aggaataata tttgtattgg cttatgccat
    12841 caatgttcat tggagcatag cccctgtttt tttattgact gttccagttc ttgctatagt
    12901 gagcaacatg ctcagtaaga aaataaaatt gatttcaaag aaaattcttg gcgagacaac
    12961 atctttagca ggagccacta ctgagtcatt acgaaatatt gagttggtta aaagtttggg
    13021 gttagccaat caggaaataa atcgcctgaa cacggttaca gataagatac tgggtctcga
    13081 attaaagaaa gtaaagtata ttcggagtct tagttttgtt cagggtacta cagtcaattt
    13141 tttaagaacg ctatttgttt ttttactgta ttatctgatt tttaaagacg agatttctcc
    13201 gggtaaaatg atgacactga tgttttattc gtttttcatt ttcgggcctt tacaagagtt
    13261 gggtaatgtc attgctattt atcgtgaggc acaggcttca ctcaccactt ttagtcagat
    13321 tttggaaatg cccttagagc ataaaccaca atcgccacac caacttaacg atattgaaac
    13381 atttgcgttt cgggatgtta gctttaaaca tatttcagca cctaacaatg cactgagtca
    13441 tatcaatttt acagcaaaaa gaggacagac aattgcattt gtggggccct caggcagcgg
    13501 aaaaagtacg ttggtaaaat tacttgttgg attgtatcat cctgcctcgg gtcaaatact
    13561 ttataatgga actgattcaa acagtataaa cttagatgat ctgcgtggac agattggatt
    13621 tgtgacacaa gacacgcaat tatttgccgg aacaataaga gaaaacctgc tgtttgtaaa
    13681 tcctcaggct gctgatgaac aaatattaaa tgcattaaag atggcatcgt gcaattcatt
    13741 attagaaaga gcagaaaatg gattgaacac catcattgga gagggtggtg tgaaagtttc
    13801 cggtggagaa aaacaaagac tatccattgc acgagctttg ctgcgaaatc ctaaactgtt
    13861 ggtactggat gaagcaacct ctgcacttga ttccataacc gaagaagaaa taacagaaac
    13921 aataaaaact atcaataaga ataaaaatct aatcaccata ttgattgcac acaggctatc
    13981 aaccatcatg catgctgaca aaatttatgt tcttgaaaaa ggtcataaaa ttgaagaagg
    14041 aacacatgat tctcttctgg cagaaaaagg attgtattat gccatgtggc gtcagcagat
    14101 tggtgagcgc aaaaagggag aaaacgttgt cacagcttga taatattaaa gactttgagg
    14161 ttgctatatc ggaaagacta aaagatttgc cggggagcaa ttctcatatc aaattggcct
    14221 cggtgaaccg ccctgtttat gagcctatgg ggaattaccg cacgggcagt gtcatgttgc
    14281 ttgtcaacaa taaaaataat cttccgcatc ttgtcatgat aaaacgcgca gaagatggca
    14341 aagtgcattc ggggcaaatt gcttttccgg gtgggaaggc agagtctgat gaaagtctga
    14401 cactaacagc cctacgcgaa ctggaagagg aaataggaat aaaaaatgac agtattaata
    14461 ttataggagc acttacaaga gtttatattc caccgagtaa ttatatggtc tatcctttta
    14521 ttgcaacaca gattaatcca tcagcttttt tagctaatgc gtctgaagtt caaacgattt
    14581 ttgaaatttc gttgcttagc ctgatagcta ataaaaagat tgttaaggat gtggaacatc
    14641 aaacaacaag gggcttaata aaagcaccag cgtttaaaat agacaatctg gttatctggg
    14701 gcgcaacagc aatgatatta tcagagttta ttgacctaat agattaagtt tttgagcaat
    14761 tgctttttat ttgaagaatg ttattatttt tacgtccata aaaccatata taatatgaga
    14821 agacatttac aaaaattagc agctatttgc atgcttggct tttttactat gcccacctat
    14881 tcgcagagat ttttaagcga ggtgtttcca tcggtcaatg taacaactaa tgttgtttat
    14941 gcacagaact atgaagtact tacggggaca cctgttttga aagatttaaa aatggatgtt
    15001 tatgaacctg caggcgctgc agacccaatg acagaaagac cgttgattat ttatttgcat
    15061 acaggaagtt atattccgat tattgtgaat ggaaatgcaa ccggatcaag aaatgacagt
    15121 gcagcagtag aaatttgcaa acagtttgca cgcagaggct atgttgttgc aaatatggat
    15181 tatcgcttag gatgggatcc tacaaacaca aacccgacaa atggacttga tattcgcaga
    15241 ggcaccttac tgaatgcagt ttatcgttca atacaagatg caaaatcatg tgtgcgttat
    15301 ttcagaaaag acgctgcaac aaacaataat gtttacaaga ttgatgtgaa caatttttat
    15361 ctcgttggtc agggaacagg tggctatatt gcattgaact atgcagtgct aaacagccca
    15421 tctgaaatta cacttcctaa atttatcagc agtacggatg cgccaaccta tggaatttat
    15481 gccggtcagc cttatgtaaa tcaaactgct ttaggcgatt ttgatggtat tggcggtgta
    15541 gcaccttata acaatgaaaa taatccgggc tattcaaatg cggttcagtt tgtagtgaat
    15601 ttaggtggtg ccatgggcga cagttcatgg attgcagcag gcgatgcacc tatgattggc
    15661 attcattgtg tagatgaccc atttgcacct tatggtgttg gaattgttta tgtacctact
    15721 actccaccac aagctgttgt tgatgtaatt ggttcaggcg aaattattaa atgggcaaac
    15781 ctattaggaa ataatgcttg ttttgctaat gcaggattta ctgaccctta taccatacgc
    15841 gccaacagta ttaataatgg taatgaagga ttgtttccac tacaacaagg aattaatccg
    15901 gtgaacccac agcttggaca tgccggtcct tgggaatggt acgatttgca atcaacacgt
    15961 aatcttgcta tggcaatggg ttatcagtca acacgcgggg atacttgtta tggcaattcg
    16021 atgttaacca acccaactat gagcaaggct tatgctttgg catatattga taccattatg
    16081 aactacgtaa atcccagaat cgttttctgc tgtaacttac cgacatccgt aaaagaagtt
    16141 tctaccaaca gttcagatgt aactatctat aacagtccat ctaaaagcta tttcaccgta
    16201 gtttcgacca gcaaagagcc aatgcgttct attgaaattt atgatataaa aggcagcctt
    16261 ttagcagctg aaaaaaatcc aaatgcattt agtcagcgaa tagaaaaagt atcgctatca
    16321 cttaacaaag gtgttgtact tgtaaaaata acttacaact ctgacgtgat taccaagaaa
    16381 ataactttag attaagtttt aaaaatttta atgagtttag aaaggcagtc tcaggattgc
    16441 cttttttatt tttcatctat cagatactga caaccgcctg tgtgaacagc aagaattttt
    16501 gttcccgtat taaattgatc ttttacaagc atatcaaata ttccatagaa caattttcct
    16561 gtgtaaacag gctctattag aatattcgtt ttttgcgtaa acaactcaca aaatattttc
    16621 agttctgcat tgcttttacc aaatccacca aaagtatatt gatgcctgat aatatacttt
    16681 gcagttgagt tactttcaga taagtgtagt tgtacgtttt tagtaagaga atcggcagcc
    16741 tgaacaacag caatgccaat tgcgatttta ttttctgaga tagatgctgc aaggcctgcc
    16801 aaggtagttc ctgttcctac agcgcagaca accgtgtcat atccggcatt aatatcattc
    16861 aaaatttccg tacagccttt ataaccttct gcattatttc caccttcagg aacgatataa
    16921 caaccatcag gatttatatt gttttcaaaa agcaaatgtt ttactgcatc acttttataa
    16981 aagtctttat catgaatttg tctataccat tggcgactga cttgaattag cttcatgcca
    17041 tcattcacag cacgttttag cgtgttattc attggcgtaa taatttcacc tcttataatg
    17101 cctacagctt ttatgttttg taatttgcaa gtacgtgcag ttgctgcaat atgattactg
    17161 aatgcaccac caaaggtaac taaagtgttt ctcccctttt cttttgctgc ttgaagattg
    17221 tattttagtt tataagattt attacctcct gaaatttcat cattcaggtt aagtctgagc
    17281 acatgcaaac tcactccttt atgggccaac tgaaaggcgt tgagatttat ttcttcaata
    17341 tttcggctat tcatttacgc aaagctaatg agttacaaat gacttgatag gaaaatgagt
    17401 tgtcattact caatctattg aactgatatg acaaaatatt atttgaaaac aaactgcata
    17461 ttgttgaact ttgcaaatga tatggctttt acagacaaat ttgaaaaaga tgaatactat
    17521 ctgactcctg aaggatatat tgtatttacc gagaaatatc atcttaagag aggatattgt
    17581 tgtcaaagcg gttgccgtca ctgtccatgg aattataatg cgaagaaagc agacaagaat
    17641 aataaacaaa agttatagac ctgcttaatg cacagtcagt aaagttggtt tttttaagct
    17701 atttttgccg cctattgaat actaattatg gcagagaaag ttaatcttga atttaacatc
    17761 aatgaagatg cttataagac acatctgatt cgtttaacag agcgtttaaa taaaatttac
    17821 gaaggtggcg gaaagaaaaa tgcagctaag cagaaagaaa aaaataaaat gcttgcacgc
    17881 gaaagaattg catacttgct tgatacagat aaaccacaat ttgaagttgg tgcatttgct
    17941 gctgatggca tgtatgccga acaaggtggt tgtccttctg caggtgttgt tattgtaatt
    18001 ggttatattt caggaaaaca atgtatcgtt gtggctaatg atgcaacagt aaaagcaggt
    18061 gcctggtttc caatgacagc aaaaaagaat ttacgtgcac aggaaatcag catggaaaat
    18121 aagttgccta ttgtttatct tgttgacagt gctggtgtat ttcttccgat gcaggatgaa
    18181 atttttccgg acaaagaaca tttcggtcgt atattcagaa acaatgctgt aatgtcatct
    18241 atggggataa tacagattgc agcaattatg ggtagttgtg ttgcaggagg agcttatttg
    18301 ccgataatga gtgatgaggc tttgatagta gataaaacag gttctgtgtt tctggcagga
    18361 agttatttgg tgaagtctgc tattggagaa gatatagata acgaaacttt aggcggagct
    18421 acaacacatt gcgaaatttc cggtgttaca gacaataaat ttccggatga tgcatcatgt
    18481 cttgattata ttaagcgcct gttttctaag attggaacat ttgacaaagc cggttttgag
    18541 agagcagcat caaaagaacc tgcagctaat ccaaaagaaa tttatggact aattccggca
    18601 gaaaattcca aacagtacga tatgcatgag atcatcaacc gattggttga taacagtgag
    18661 tttgatgaat acaaagaaaa atatgggcaa agtattttat gcggcacagc acgtattgat
    18721 ggatggtctg taggaattgt tgccaatcaa cgtaaagttg ttaaaagtaa aaaaggcgaa
    18781 atgcagtttg gcggagttat ctatagcgac agtgcagata aagctgcaag gtttataatg
    18841 aactgcaatc agcgaagaat acctttggtg tttctacaag atgtaaccgg ttttatggtt
    18901 ggtagtcgca gtgaacatgg gggtattatt aaagatggag ctaagttagt tagtgcaatg
    18961 gccaatagta ttgtgcctaa attcaccatc atcattggta acagctacgg tgccggcaac
    19021 tacgcaatgt gtggcaaagc ctacgaccct aggttaattg ctgcatggcc aacaggacaa
    19081 atagctgtga tgggtggttc acaggcagca aagactttat tgcaaattca ggtggctgct
    19141 cttaaagcac agggtaaaga aatttctgaa gaagacgaaa aagcgcaact aaaaacaatt
    19201 accgacagat ataattctca gacttcgcca tattatgctg catcaaggct ttgggttgat
    19261 gccattattg acccattaga tactcgtaaa atgattagca tgggcattga aatggcaaac
    19321 cacaatccaa tatccaaacc ctataatgtt ggagtaatac agacttgatg ctttgggaat
    19381 aagcaagcat aaaaaagcgg aaacatacag atgcttccgc ttgaattttt acatttgttt
    19441 tattaatgct tgtgattggg gtcactatga tcatgacctt ttacatcatt actttcacac
    19501 atacgggcaa tttctgtaaa aatggaattg gtctcaccca attttttagt gatagattca
    19561 tcactttcat taagttcaat aagtccaaac aattctttgc aggcaactgc aagtcttgtt
    19621 gttacatcac tcattttagc agcatcaatg cctgcaggtg cttttgattg atgcaaagca
    19681 acggcaagtt ccatcagttg tttgtactct gctttaattg cttttaaatt accatcctta
    19741 cttggctggt acgtctcagt caaaacttta tgaaagttgt ttaactctgc ccatgaattc
    19801 atggttttgt tggcctgact gtttgctgtc aaactgaatg aagcaagtgc aatgaataaa
    19861 agaatccttt tcattttatt tgatttttat ttatgcaaat ttataagatt ccaacctaaa
    19921 acaaaacagt tttttatctt tacccgatga gtagatttat ttatagtttt ttctttaaac
    19981 tttttggctg gaaaataaaa ggaaacatac ccaatgagtt aaaaaaatat gtaatgattg
    20041 tggcaccgca taccagcaat attgactttt ttgttggctt agccactaga agtattctta
    20101 gacttgacac caagtatctg gctaaaaaag agttgtttag acctcctttg ggttggattt
    20161 tttatgcatt gggcggatat cctgtggatc gttcaaaacc gggaaaatta gtagaaaaag
    20221 ttgctgaaat ttataacaac cacgataagt tctcaatctg tataacacct gaaggcacaa
    20281 gaaaatatgc ggctgagtgg aaaaccgggt ttcataaaat agcaatggct gtaaaaactc
    20341 ctgtggtgat ggttgctttt gattaccgca ccaaatctgt tgtcatagcc cctcctttca
    20401 caccttctga tgatacacaa ggtgatattg aaaaaatcaa agtctggtac aggcaatttg
    20461 aaggaaaagt accggaatat ggtgttaaat aaaaaaacac cataagtatt tgataattca
    20521 gctaaaagtt agatatttgc agccccaaaa tcagggagga tcttgggata aataaattta
    20581 aaaatcatta aaaattaaaa aatgccaaca aaaatcagat tacagcggtt tggaaaaaaa
    20641 ggccagccgt tttaccatgt agtggtggcg gattcacgtg ctcccagaga tggcaagttc
    20701 attgaaaaaa tcgggacata caatccaagg actatcccgg caaccattga ccttaatctc
    20761 gacagagcac tctattggtt aggcacagga gcccagccaa cagaaacaac ccgtgccatt
    20821 ttaagttaca aaggtgtatt gtacaagaat catcttttgg gcggagtaaa aaaaggtgcc
    20881 ttaactcaag aacaggccga tgctaagttt agcgcatgga tggaaaacaa aaaccttaaa
    20941 gttcttcagc attcacaaaa agtgcagtca actaaagcag atgatatgaa aaaccgcttg
    21001 gttgaagagt ctaagaaacg tgaggttatt gctaaagcta ttgctgaaaa acgcgctgct
    21061 gctaatgttg ctgaagccga agctgctgct gaagcacctg caactgatgg aggcgagacc
    21121 cccgcagaaa acgcataata ttattcttat ataatgccct ttgataatgg gttttgatga
    21181 ggttactttt tttggaacaa ttgttaaaac acatggtatc aaggggcatt tggttttaag
    21241 atgctacgat aactttaagt gtaaaaagaa caaccctgtt tttctttttt ttagagaagg
    21301 tgatttatta aaacaagcag cagtttcaga aatccaattc acatacccct tttgcagaat
    21361 aaaatttgct gacattgcag atataaatga agctgaaaca tggcttaaaa aggaagttta
    21421 tattgagaaa aaagcactgc ttcagaacga aattccatat aagtttgaat tgataggatg
    21481 ttctgtcact gatagcattt caggactaat cggtaacgtt attgatatag cagaataccc
    21541 acaacagcag atagccatta ttaatgccaa tggaaaagaa gttttaattc ctcttaataa
    21601 aaatttcatt actagattca atagcactga aaacaattta gttgtaacac ttcctgatgg
    21661 tctgcttgac atttatcttc agtagttcaa tcatattttc acgtatttta tccttattaa
    21721 aacatagctt gatttttagc tttaaaccgc attaaagatt ctctcaaaaa acattgtaat
    21781 aacttgacac gttatttact tttttaactc ctattttttc agatattcgc aacaaattat
    21841 cggggacgaa tggagattaa atatatcgga gagcagttaa tatggggcta cataggcaat
    21901 ggtttcattt ttactgcatt tatcagtgct atatatgctg cagtattata tctggcaagt
    21961 gtaagaaatg tggagaaagc tcaatgggca acacccttag caagaaaatt tttatacctg
    22021 catagcattt catgctttgg aatatttttc cttctgctct atctgatctt caatcaccgt
    22081 tttgaatatt attatgtatg gcagcactcc aacatggcta tgcccatgta ttatatcctt
    22141 gcttgcattt gggaaggtca ggaagggagt tttttattgt ggatgttatg gcatagttta
    22201 attgctttat ttatttcaag aaggaaccgt cattgggaaa gcccggttgt aggaaccatt
    22261 atgatggtac aggtctttct gggttcgatg ttgttgggcg tttatatatt cggatataaa
    22321 cttggatcga atccttttac tttacttcgc gacaacgatc aatttgccaa ccttccgttt
    22381 ataaaaatgc ctgattatct ttcgcgtatt gctgatggtc gtggactgaa ccctttgttg
    22441 cagaattact ggatggtaat tcaccctcct actttatttt taggttttgc ttctacagtt
    22501 gttccctttg catttgcatt aggtgggctc atgcgcaaaa acattactga atggattaaa
    22561 cctgccctgc cctatgcatt cttttcaatc atgaccttag gtgccggtat tcttatgggt
    22621 gcagcatggg cttatgaagc attaagtttt ggtggtttct gggcatggga tcctgtagaa
    22681 aatgcatctc ttgttccatg gcttgtaatg gttggtgcag gacatgtcat gcttgtgttt
    22741 aacaaacgtg gacgggcttt actcagtacc tacatacttt gtatcctctc atttttgctg
    22801 gtgttgtata gtacttttct tactcgtagt ggaattcttg gagaaacatc tgttcatgca
    22861 tttacagacc ttggcatgag tggtcagctt attttttata tgctgttctt tgtcgttctc
    22921 gctattgtaa tgcttgccat aaactggaaa aaaattccta aagaaaactc agatgacagt
    22981 attacctcac gcgaattctg gatgttcatt ggaatgttgg tgctttgcat atcagcaatt
    23041 cagattacat ttactacaag cattcctgtt atcaataaaa tattcggctc aaaccttgcc
    23101 cctcctgttg aagtaaaaga acattttaac tcctggcaaa tgcctatagc tatgattata
    23161 gctttactta tggcagttgg acaatttttt aagtataaag aaagcaaccc aaaagaagta
    23221 tttaaaaagt tgttgttatc tctgaccatt tcattaattc tcgctgtagt tttaattctg
    23281 ttgttaaagt ttaacagtat tcattatatg atattgttat ttactgctgt atttgcaatc
    23341 attgccaact tcgattattt tattcgtgta ttaaaaggga aattctcttt cagtggtgca
    23401 tcaattgcac atatcggatt cgcttttatt ttgttaggca ctttaatttc aaatgcaaat
    23461 tcaagaatta tttctaaaaa cagattgaat attgatttag gaaaagactt tcctaacaat
    23521 gaaaacatta tgcttgccaa aggtgatacc ttgcagatgg gaaagtattt tgtcacttat
    23581 agtggtcgcg aaaagaaagg tcataacatt tattttgatg ttgaatatta taaattgaat
    23641 aataagacag gcgaactaaa aaaggcattc agcctgaagc cgattattca acttaatgag
    23701 agaatgggaa attctcctga accggcaaca aaatggtttc ctgataaaga tatttataca
    23761 cacgtaacct atgctaatct ggacgaatac actaaaaatg tatccatgga cgagtatgat
    23821 acacctactg aacatgagat ggctgtaggt gatactttgg ctacatcaaa cagtcttgtt
    23881 gttttggaaa aattagttaa agacaatact ggtgttgaag tagaatcatt tgacagtttt
    23941 ggtgtaacag ccttgttgga agttactgat gtaaacaaaa agaaacatac aataacccca
    24001 caatttctta ttaaagacag aatggctgtt tcaaaaaatt attttcttga tgaacttggc
    24061 ttaaaattca ggttcacaag catcagtcct gaaagcggaa aaataaaact tgaaattgct
    24121 gaaaagaaag cgaacaaaaa ggattttatt attatgaagg caattatttt ccctcatata
    24181 aatttattgt ggattggttg tatcttaatg cttacaggaa cttggatagt ttcagtaaaa
    24241 cgatttaaag aatatgtccg ctacaccgac aaaggataac agcatgacac tgaccattat
    24301 tggatcaggg aatgttgcct ggttgttggc catattattc aaaacttcag gacataagat
    24361 taatcagatt tacagccgaa ataaaaaaac cggttctgca ctttcaaaaa ttacaggcag
    24421 taagtttatt gctgacgttc aaaaaattga catcaaatct gatatattta tcattgcaac
    24481 caacgatgac agcattgccg aagttgtatc taagttacct gatataaaca gtatatttgt
    24541 tcacacatcg ggcactgttg atttgcaagt cttgaaaaag aaatttaaaa aagctggcgt
    24601 agtttatcct ttgcaatctg ttgcctccga aagcgttcta tcagacgaat taaccttttg
    24661 tttggaagca actaacaaca gggttttaac ttcactgaaa caacttatta cttctataaa
    24721 ttgcggctat gtggtgatga actctcagaa aagagcaaag ctgcaccttg cagcagtgct
    24781 tgtgaataat ttcacaaatc atttgtatgc acaggcattc gattttctga aaaaaaacag
    24841 aattgaattt aatctactaa aacctttaat cagacatact gtaatgcgat tagatggtaa
    24901 tcctgcttct tttcaaacag gacctgctgt cagaaatgat aaaaaaacaa tacaacgaca
    24961 tttaatgtta ttgaaagata acaaacaact tacaaaactg tataagcaaa tgacggattc
    25021 aatttataaa tttcacaaaa aatgaatagc gtgaatttta aagaaaaatt aaaagatatc
    25081 aaatgctttg tctttgatgt tgatggtgtg ctcactaatg gcatgctgca tgtcacaaac
    25141 gatggaatgc ttttgcgtgc tatgaatatt aaagatggct atgcccttca attagcagta
    25201 aaaaaaggtt atcgtgttgt aattatttcc ggtggtaagg atgagggcgt tgttatccga
    25261 cttaaaaatt taggtataac tgatatctat gttggcatag gcaacaaact tcaacagtta
    25321 cttgctgtaa tgcaggaaca tcatcttaca tcagctcaac tactttatat gggtgatgat
    25381 atgcctgatg tagaagcaat gaaaaattgc tctattgcca catgcccata cgatgccgca
    25441 cctcaaattc gcgatatttg ccattacata tctcctatta aaggcggtga tggatgtgca
    25501 cgtgatgtta ttgaacaggt tttaactcta aacaacgatt gggaataatg ggatttctaa
    25561 aacttatcag gtttccgaat ttaatcatat tggcattagc gcaatgtctg atatattata
    25621 tggttgttgt tcctttactt aaaacatcag aatttacacc ttcgttacat acatggcagt
    25681 ttctactgat tgtgctaagc acagtactta ttgcagcagg aggttatgta ataaatgact
    25741 tttttgataa agacattgat gccgcaaacg gaattggtaa ggttcacgat ttttccggat
    25801 ggacaatgaa aacactctat tttgttttct ccatcatagg aattgcaata ggactttatc
    25861 tgacttatgc attgaagtta agacaatttg cactaaccta tttactcact gcagcactac
    25921 tctattttta ttcagcttca tacaaacgac ttcctttggt tggaaatttt gtggtggcat
    25981 ttttaactgc tgttgctgtt tttcttccgg catttgcaga ctatgagttg cagtatgcat
    26041 ttcgagatat taagcttcct gtaatcaata ataagatgta taatcttaga ttaataattg
    26101 caattgcggc agcgtatgca ttttttgctt tccttatttc attagtcaga gaaataataa
    26161 aagatatgga agatattgaa ggcgataggg aatttggatg caacaccctc cctattgttg
    26221 ccggaatcaa taacacaaaa cttattgcca ttggtttgtt gcttttaata gttgggctga
    26281 ttttatttat tcagattaaa caagcctggt gggaaagcct tccggtattc ggttatacta
    26341 ttctatttgt acaagtgccc ctgctcattc tatcagtgaa attatttctc tcggctgaaa
    26401 aaaagcattt caaagtagcc agcatgattg ctaaagtggt tatggttggc ggaatacttt
    26461 cattaccggt tttcaaattt ttcagtacct gacctttttt taataaatga tgttattagt
    26521 ctatacacat aagttttcac cacgtcttaa atatattttt gatttattta ttggcaaact
    26581 aatgggtatt agttttgaaa taacacataa tgttgaagaa tttaaaagtt atgccggccc
    26641 gaaatttaat tactcagaac atcagattga aaatgaaata ttcatttatt cagagaggtt
    26701 gttatttgaa aaaggcatcg aagaccagca attcggcttt actgaatggg atggcataaa
    26761 agcattttat gcaacacatc caaaatacag tgttcctttc gatttgtttt ctgcatcatt
    26821 ttatttggtg agccgttacg aagagtatct gcctcactta cgggacttgc atgaccgcta
    26881 taatgaaact gaaagtattg catatacaag aggcttttta cagaaacctg tagtaaatat
    26941 ttgggcacag aaattcaagt caattatttt agaaagatat cctacgctta aatcagtcag
    27001 tccgaaatac aagtatgttt caactattga cattgacaat gcttatgctt atttggaaaa
    27061 aggtctgatg cgtactattg gcgcttatgg acggtcttta gtcaatttcg atttgcctca
    27121 aattgttgag cgttctaagg ttttaatgcg attgatgcat gacccttatc atacttatga
    27181 gttaatgcat gatttgcata aaagatataa gattaatgtg atttattttt ttctattagg
    27241 tgaatatgga gaaaatgata aaaatgtttc tgttgataac agaaattttc aatcactcat
    27301 tcaatctttg gctgactatg ccgatgcagg tattcatcca agctatggtt caaatatcaa
    27361 agatggccga ttacaaaaag aagttcagct actgacaaaa attctaaaaa gagaagtgac
    27421 caaaagcagg caacattttc tgaaaataag gctgccggat acttacagaa aacttataag
    27481 tgttgatatt aaagaagatt tcacaatggg ctatgctgga aatatcggct tcagagcagg
    27541 catttgctct tctttttatt tttatgatct ggaccaggaa gttcaaactc cacttttaat
    27601 tcatccgttc gctgttatgg atgcaacttt cagatattat ctcaaagtta atcctgaaca
    27661 ggttattgat ctgataaaac cattaataga agaagttaaa gcagtaaatg gtacttttat
    27721 cagtttatgg cacaatgaat cactcagtga gaatcatata tggaaaggtt atagagatat
    27781 atatgaagag ttacttaaaa tagcggctgt ctgaaatgga aataaggtat cttgaacata
    27841 gtgagattga caaagcaaaa tgggatctaa acatcaacca agctttaaat aaactgccgt
    27901 atgctttctc atggtacctt gatgtagtta gccctaactg gcatgcatta gtaattgatg
    27961 actacaacta tgtatttcca ctgacatgga gaaaaaagat gggattcaat tatctgtacc
    28021 agccaatgtt tacacaacaa ttaggaatat tttcttcaca ccctgtagat tatgcaactt
    28081 ctaatgcgtt tttaaatgct attccatcac aatttaaact gatagagata aatttaaact
    28141 ctttcaaccc ggcagctggc aataaattta ttgctaccaa acaactaaac ttcgaattgg
    28201 agctaaacaa accttacgaa gaactcagaa agttttactc tgaaaatcaa aaacgaaata
    28261 ttaataaggc aaaaagatct gaattaagtt taaaccaacc tgatatttcc gaagtaataa
    28321 gcttattcag acatgacaga ggtttgaaaa tagaacgaat gaaagatgaa agttatgaat
    28381 tactaagccg attattttct actttgaaac aaaaaggaat atgctataca cgaggtgttt
    28441 ctgatggttc tcaaactatt tgtggtggat tattcattca aacagattat agaattattt
    28501 ttcttttttc agggaatagc gtaattggaa aatcttccgg tgcaatgtca ttactcattg
    28561 actctgtaat tcaggaaaat gcaaacaaga atatagtcct tgattttgaa ggctccaatg
    28621 atttcggact ggcacgttat tacagtagtt ttggttctat tgaacatgta tatttacacc
    28681 tgaaaaaaaa tacacttcca tttcctgtaa gacttttaaa acgataacta tggtaaacat
    28741 actaagcaca agcaactcct tattgcatca ttttgtagca gaattacgcg atgttgaaat
    28801 tcagaatgac aggatgcgtt ttagaagaaa cctcgaacgc attggtgaaa ttgctgctta
    28861 cgaaataagc aaaaccatga ttttcgaaaa caccgatgtt accacccctc tgggcattgc
    28921 cacagtacca ttgatgcaaa gccaaccggt attgggcacc attttaagag caggactacc
    28981 attgcatcag ggattattga attatttcga tcaggcagac aatgcattta tttcggctta
    29041 cagacgtcat cataaagacg gcagttttga tattcatctt cagtatcttt catcaccacc
    29101 cattgccgga cgtattttaa ttttaagcga ccctatgctt gccaccggtc agtcattagt
    29161 agaaacatat aaagcaatgc tcgaatcggg gcagcctgca catacacacg tggttgcagc
    29221 tattgcaagc agacaaggta tagaatatgt taaagcaaat ttacctgatg atttaaccat
    29281 ttgggttgcc gctgttgacg aggaactcac agcacagtca tacattgttc ccggtttggg
    29341 tgatgccggt gacttagctt ttggcgagaa aaagtagttc aaacagaatt ttcactatct
    29401 ttcgttggtt aatcagaata ttaaaacatg ggtgagtttg taaaaattct gatggtcata
    29461 ttaatcagca gtgtaaaatt tgttgttgga cctgcctttg cctattatga cgagaagtat
    29521 gacttcacaa ttttcgaagc aatagtctat cctattatcg gtggaatgct tggtgttttt
    29581 gtctttagct tttttagtga tcaggtagca aatgtttggc attggctgca aaatcgaata
    29641 aaaaaaaccg tttcacgtaa ccaacctttc actgaaccca cagtagatag tgaaggtaaa
    29701 gttgttgtca gttatactta tgttgacgct aaggaaaaac caaagaataa aatttttact
    29761 aaacgaaaca gaagaattat aaccatttgg aaaaaatatg gtttgtttgg aattgctttg
    29821 ctaacgcctg ttattatttc aattcctata ggtacaatca ttgcgtcaag attagtacct
    29881 gataaaaaga aagttttttt atacatgctt atttctattt cagcatggtc aattgtaatg
    29941 gtatcttcgt ttgaactata tcatgcctat tccataaaag ctttacaaca aaaagttctt
    30001 gaaccatgaa aaagttaaag cactattcac atcttatttt tgacctcgac catactttgt
    30061 gggataccaa tgtcaacgct gcacaatcgc tgtctgaaat gtatcatcaa tataatttag
    30121 ggcagtatgg cattatcagt gaacatgatt ttattcagaa atatgttgac attaacaaca
    30181 ggatgtggac tgaatattct cttggacgga ttaaaaaatc ttatctgcgt gcagggcgat
    30241 ttgaactcac tctaaaatac tttggaattg caaacaagga gttagttgaa aaactttctg
    30301 atttttttgt tcagaataca cccggcaaaa aggagttaat tccatttgct aaagatttat
    30361 taaatcatgt acatggcaaa taccagttag ctattattac taatggcttt gctgaagcac
    30421 aacacaccaa gttgcgagca tcatcaatag atcacttttt tagtaagata ataatttcag
    30481 aagagattgg ctatcacaag ccagacccaa gaattttttt acaaactgca gagttacttc
    30541 agacaccaat aaaagattgt ctgatgattg gtgataatgt agagactgat attgccggag
    30601 cgattgcctc aggtatggat tgtgcatatc tgaatcctgc cggagtcaaa cataattacg
    30661 aggtaacttt tgaaacgcat tgcctgagtg aattgattaa atggctttaa tgttatgcaa
    30721 acttttgaag gtgtagtcgt aaaaacaaca ggtaatatta taaccgttca aaccggaaaa
    30781 gaaagaattc tttgcagaat taaaggtagt ttcaggatta aagacattaa cagcaccaat
    30841 cccgtagccg ttggggatta tgtaaaatat tattttgaaa acgatgatgc attgattaca
    30901 gaacttcttg acaggaaaaa ctacatagtc agaaaatcca aaaaattaag caaacaggtt
    30961 catattttag ctgccaatat tgatcatgca tatttagtag ctacacctgt actaccacga
    31021 acatcaacag gatttattga cagatttctt gctactgccg aagcctattc cattaaggct
    31081 tctatcattt ttactaaagc agatatttat gataaagaca ttttaaatta tgttaatgaa
    31141 ttaaaaaatc tttattcagg aattggatat tctgtttatg tagtgtcaag ccacgtatca
    31201 gattcattaa gtccgttaaa ggcacagtta aaagataaaa taaatttatt cactgggcac
    31261 tcaggagttg gaaaatcttc tcttattaat gtgttaattc ccggtttaaa tttgaaggta
    31321 acgccaatat ctgaacaaca tctgaccgga aaacatagta ccacctttgc agaaatgcat
    31381 caactcccgg aaggtggatt cataattgac acaccgggca tcagggagtt tggaacttac
    31441 gatttcaaca acaatgatgt gacacattac ttccctgagt tttttcgact attaccaaat
    31501 tgcaaattca acaattgtat ccacgtcaac gaaagcggtt gtgcagtttt agcagctttg
    31561 gaacatggtg agattagtcc atcacgctat cataattacc tcagtatttt aaataatgaa
    31621 gatatatttc gttaaacatg aaagttgtca tacaaagagt aactgaagca agcgttgaaa
    31681 ttgaagaagc aatttattct tccattaaaa atggtctgtt agtgcttttg gggatttctg
    31741 ttgctgatga aaaagaagat gttgattggc tatgcaagag aattatcggc ttgcgaattt
    31801 tttctgatga aaacggatta atgaatcttt ctgtaaaaga tattaatggt caaatattgt
    31861 tggtgtcaca atttacactt tatggtgatg ctaataaagg taaccggcct tcatttattc
    31921 aggcagccag accggagaaa gcaattccat tgtatgaata ctttattcag caactttgta
    31981 aaaatgatat caatgtttat acaggaaaat ttggcgcaga catgaaagtg tctttgacca
    32041 atgacggacc agttactata attatagact ctaaaaaaca tacaaatgac cattaaagat
    32101 tctcaacaaa ttgttgacga ttggataaag aaacatggtg tccgatattt caatgagtta
    32161 acaaaccttg ctttactaac agaagaagtt ggtgaattag caagaataat cgcaaggaaa
    32221 tatggtgagc aatcagcaaa aaataatgaa gacagtttac agatggctga tgaaatggct
    32281 gatattttat ttgtattgat ttgcttagcc aatcagaccg gcactgattt agaggaagct
    32341 tttaaaaata atcttataaa gaaaacaaat cgtgatgcaa cacgacataa agtgaataag
    32401 aaattacttt agtttgtcag ctctattacg tccaattaaa aaatcaattc caaaactcaa
    32461 aactaatttc tgacggttaa attccaactc attatcaatt ttaaaagaag gggtgatttg
    32521 aaatttggct tttactaagt ttcctcctaa tgctaaatac aaacttggtt catagaatag
    32581 aaatttttct ccgtaaagca tatcatcatt gtctgcctga ctataatatt tcatatcctc
    32641 gtaaagtagc ctgctaagcc tgaacacaag tccaaaatca aatctcctag ttctgtaaaa
    32701 taccccgggc tgaatataat atcgcatcaa ttcagaacta aaaaacttca aactatcctg
    32761 attcattctg atactcccct ttcctattcc ggcataaaca tctgcgcccc atctttcgcc
    32821 agccattcca taatatccaa gtccgacatt gcctacaatt cctttccctg aataatcatt
    32881 tgttttatct ccaccactga aatacatgcc gtctgcagta agcccaagat gctttatggg
    32941 tgagtaactc atctgtgcat cgaaagccat gccgccaaca ccaaactgag gtaatgaaaa
    33001 cgaagttccg aacttgcctt cacctccatt tgccaatgca ggtatattgg gctgaggtgg
    33061 tacataatac ttaggcgaac aaccggatat aagaaacgtc acagcaataa caataaatat
    33121 gtatatagaa tcaggtttca taaattagat aattgaacgt aagtcaaaat tacaacttca
    33181 aattgaacaa atagtcaaat ataaacacac ttgtaaattt ttctgcatta aaatgatttt
    33241 gtctgatgaa gtgttttgaa tcttgtaatt ttgaaacgaa aaattaattc atgtcgaatc
    33301 aaaaggctat tcagaccacc tggattagta tcattggtaa tactttactt gcaggcatca
    33361 aatggattgt tggctatttc ggtaattcgt atgcattaat tgctgatgca attgaatcta
    33421 ctacagatat tttctcgtca attttagtgt tgcttggatt gaaatatgct agccgccctg
    33481 cagataaaaa tcatccctat ggtcatggtc gtgtagagcc actgataaca tttgttgtag
    33541 ttggaatttt agtttctgct gcaacagtta ttgcaattga atctataaaa aatatcagaa
    33601 caccgcatca gataccggag acctatacac tagctgttct ggctgtcatt atagtgagta
    33661 aagaattatt ttatagaatt gtttctaagc ggaatgaaaa aattaatagc tcagcgctaa
    33721 aggccgatgc atggcaccat cgaagcgatg ccataacttc tcttgctgct tttatcggaa
    33781 ttgctattgc tatttattta ggtgatggtt atgaggctgc agatgactgg gcagctttag
    33841 ttgcttccgg atttattttt tataacagtt atctgatatt cagacctgca ttaggtgaaa
    33901 tcatggatga acatctttat gatgatgttg ttaaagaaat cagagaagtg gcaggaagag
    33961 ttgaaggtgt gattgataca gaaaaatgct atgtgcgtaa aaccggtatt aatttttttg
    34021 ttgaccttca tgtcacggta aactcaaaca ttagtgtcaa ggcaggccat cagattgcac
    34081 atcatttaaa agatgcatta ataaaacaca acccccttat agcagacgtg ctcatccatg
    34141 ttgaaccgga tgagcacgat taaattttaa aaaagaaaca gctattacta ttgccctaag
    34201 taggctttaa gtaatttgct ctttgaattt tggcgaagcg tcttaattgc tttttcttta
    34261 agctgacgta cacgttcgcg tgtcaaatca aacttttcgc ctatttcttc caatgacaaa
    34321 ccatgctcaa caccaatacc gtaaaaaagt ttaattacat ctttttcacg ctcaggcagt
    34381 gtttctaatg aacggttgat ttcagtgtgt aacgactctg ccatgagtgt gctgtcagca
    34441 taaggtgcat caggattggt taatacatca agtaatgtat tatcttctcc cggtacaaat
    34501 ggtgcatcca ttgatacgtg acgacccggc attttcattg tgtatgcgat tctgtctgta
    34561 tcaatttcta attcattggc taattcttct gctgttggtt cgcgctcaaa tttttgctca
    34621 aggttagaaa atgctttctt taccttgttg gcagcaccaa tctgattaag tggtaaccta
    34681 actatacgtg cttgctctgc cagtgcctgc ataatagact gacgaatcca ccatactgca
    34741 taagagataa atttaaaacc tcttgtctca tcaaaacgtt ttgctgcttt tatcaaacca
    34801 agatttcctt cattaatcag gtcaggtaaa ctcaaacctt gtgactgata ctgcttagct
    34861 accgaaacaa caaatctaag gttagctctt accaatttct ctaatgctcg ttggtctcct
    34921 gctctgattt ttcgggcaag ttcaacctcc atctgtgctg taattaactc ctctttacca
    34981 atttcctgta aatacttttc aaacgatgcg ctttcacggt ttgtaatgga tttagtaatt
    35041 tttagctgtc tcatctaatt tatctaattt ttatgtgtgt ttctatgtat gcaatgtaag
    35101 aacaaaaaac gataatcatg gtttaaaatt gcattatttt tttaaaggcg ttgcaaatat
    35161 actatgcgtt tagtggcaat gctaacttta tttttaatca acattataca atactgctta
    35221 tcaatatatt atctttattt tttaaagtaa aagactactt tattaacagg tttttagctt
    35281 tatttaccct ttatttaaaa ataaaaattc acgtaaaagc actaaacctg attaaaacaa
    35341 attcatcttt aatacaaatt tcattcaatt gtgcttttaa aagtacttgt aagaatcaga
    35401 gtattattta ggattttcag aagccttcct ttccactcct ttaccataaa aaggacaatt
    35461 tagaacttac tgaaaaaaaa ttcttctaca ttcgtgttac atttaaataa aaattaaaaa
    35521 tagttgtcat ttaataacaa ccaacatatg ataaaaacaa ctaaaatttt tgctgtatta
    35581 gtatgtttat tagtagttac ttcattgact gcatttggac agaaaagcaa ggtaaatcaa
    35641 gagcaaaaaa ttactggtcc cggaaaatgt ttaataaaaa cagatagtct tttaaccaat
    35701 gaactgacaa aagaagacct tataaaatgg gtagaaaatc caccatttat tgttaattgt
    35761 caagacacaa aaacttataa actgaagttt tacgaattta caattctaaa aatgaagcct
    35821 tttgagaata agagttttgg tgttggcgat gataggatga tacccattct tggacgcaag
    35881 gcaattaatg atttaaaaga aggtgacact gtaatcctaa agggtgttga cctgattgaa
    35941 gatggtactg ataaaactgc aaaagcacca accatcagta ttaaaattat tgagaagcag
    36001 ctttaaaaat aaatgggatg aaaaagccac tgcccttttg aattaatttc aagtgcaggc
    36061 aatggaaatt ttataaatcg ggtgacttca aaaaacaatt tcaatgcttt tgacttggtc
    36121 tttattttag tcaggtcaat atccggtgct atataaattt gtctgtaccg tttgaaatta
    36181 ggaatcgggt tatttttata ttctgtagca ttatcaaatg ctccggtcat accatcagca
    36241 ccaatgccaa cagctatatt gagccatgcc ggaattactt ttggtttctt tataaaagag
    36301 ttgatattag cagacaacca caatgtttgt ccgttataat ctttaaaaag ttgttgaata
    36361 taattttcac ccaaaaggtc cttacgataa tgagcatact ttgattgatg aaaagaaaac
    36421 ttaaactcta ttctttgctc ctgccaaagc agctgttgtc caataaaaac tgatgatcca
    36481 agtgtgttag ctgcaatatc accccaagaa aaaccccatg cagaagaaaa gccatctaaa
    36541 gcttcgaccg atactaaaaa aatccagcca agatttcctc ctatgagtgt tgctttttta
    36601 ttgtccatgc ctgcccagcg gcaaacattg taaccttgtt tacctgcaat atacgaacta
    36661 aaagtatgtc ctgttttatc catcagtagc cattccccat tatcgttaaa aaaatgaaaa
    36721 tggttcatat catacccact ataccacaac tgatataatc ctgccatagt acctgcatac
    36781 aagccaagtg tagtatatcc tgcaatttgt actcgctgtt tgttataaac tggcgaaggt
    36841 tcaaagaatg gaattttttg ttgtgtgtta acagagtcct gacaaaatgt gttagaaaaa
    36901 atgccaatca gcaatatcac aacatgccac tttctaataa agtgaaatgg taaaaatctg
    36961 taaatggaat ttaatttctt tggtttcaac tcacgcttct aaaatcagca gcaaaactaa
    37021 actaaatgta aatgaaattg gaaattacaa ttcatgtgta ttatacaatg taatttcaaa
    37081 gattaccttt catttcaatt aagaaagctt ttaaccggtt caaagcaaat ttcacatgac
    37141 tgttatcttc tttaacctct gtaatttcct ttactccaga tggaggaatt gatttgccat
    37201 ttgttttaag atactgtttg gcaccgttta atgtaaatcc cttttctttt accaaatggt
    37261 aaacaactcg gaatgcttca atgtcttttt ttgtataatg ccgctcccct ttcaagttct
    37321 ttttaggatg tagcatagta aattctttct cccaataacg caagagcgaa gtgtttactt
    37381 taaacatatc agcaacttca ccaatgctgt aatacagttt ttcaatatgt aggtcgtcat
    37441 gctgcatcat cagatgattt ttctataatt cacaaatcta atcgaatgat tgattgtatt
    37501 ttgaagaaat ttccaacacc tgttcatatt gtgccgctgt aaggtcgttg aagaagaaat
    37561 ttatcggatt tatcttgctg ccatttttta ttacttcgta gtggcagtgt gggcctgtgc
    37621 tgcttccggt attacctaca tatccaataa tctgccctct gattactttc tgtcctggtt
    37681 taaccacaat tttgctcatg tgtgcataca gcgtttcata accaaaacca tgatttatgg
    37741 ttacattatt tccataccct ctaccctcct tttcaacctt ttttaccaaa ccattgcctg
    37801 tagcatatat ttctgttccc accggggcgc taaaatcaat accggtgtgt aaaatactgg
    37861 tcttgtaaat gggatgaatt ctgtagccga agcccgatgc aatccgcgat aattctttat
    37921 tgctgacagg ttgtattccc ggaatagatg ccaataactc ttttttattc tttgcgagtt
    37981 caaatacctc atcaaacgat tttgactgta catacatttg ccgcatcagt ttatccaact
    38041 gtgttgttgt ttcaatcatc agcgctgagt tgtcataacc ctttaatgaa cgatacttat
    38101 caacgcctcc ataacctgcc tctcttacac tttcgggtat tgggtctgct tcgaagatta
    38161 ctctgtaaat attgtcatca ttatactcaa tatccttaag tactttagac atcaagtcca
    38221 atttttgttg caaaatctca tattgaaggt ccataacagc aatttcacgt ttaagctgct
    38281 tttctttagg tgaatccaga aactgatagg ccaatgcgat aatgattgca ctaaatacca
    38341 tagttgttgc cagccatcct aaaacacgta aaaagcgctt tttaaaggaa acaactacct
    38401 tttcgtattt taaagaatgt gtattaaaat aatatttggc cttcgccata atcttaaatt
    38461 ctatttttgc acaattcagc aaaaataaaa ttttcctgat attgcataaa tattcaaaaa
    38521 ctataaaaat cagttattta aatatcttca tttaaacttc tttcaccatt ttaataaatt
    38581 acatgacctc ttcagaaatt cgtaaaacat ttttagattt ttttcattcg aaacaacata
    38641 ccatcgttcc ttcagcaccc atagttgtta aaggtgaccc tacactaatg tttaccaatg
    38701 caggaatgaa tcagtttaaa gattggtttt tagggaatca acctgcaaaa tacagacgtg
    38761 ttgccgatac acaaaaatgc ctgcgtgttt caggtaaaca taatgacctt gaagaagtgg
    38821 gtgttgatac ttatcaccac accatgtttg aaatgcttgg taactggagt tttggtgacc
    38881 caaccgatcc taaggcaggc tactttaaaa aggaagccat tgcatgggca tgggaacttc
    38941 ttacagaagt ttataaacta cctaaagacc gtttgtatgt taccgttttc gaaggcgata
    39001 agaatgaaaa tttattgttc gatgaagagg caagtaattg ttggaaacaa caccttgatc
    39061 attcacggat tttaatgggt aataagaaag ataatttctg ggaaatgggt gatacaggac
    39121 cctgtggccc ctgttctgag atacatgttg acttgcgaaa tgaagaagaa agaaaacaga
    39181 cagatggaaa acttttagtt aatacagggc atccgttagt tattgagatt tggaataacg
    39241 tatttatgga gttcaacaga aaagctgatg gcacgcttga aaaattgcct gcccggcatg
    39301 ttgatactgg tatgggattc gagcgtttgt gtatggcaat tcaacaaaag aaatcaaatt
    39361 atgacacaga tatttttcag cctaccataa attatattga agcaatgagt ggcataaagt
    39421 atggcagcaa cgaacaaacg gatattgcca tgcgtgtctt atctgatcat atcagagcta
    39481 ttgcatttac gattgcagat ggacaactgc catcaaacaa caaagcaggt tatgttattc
    39541 gcagaatact acgaagagct gttcggtatt cgtttactta tctgaacttt aaaactcctt
    39601 ttattcatag gcttacacca ttattagctg atcagtttaa agatgttttc cctgagcttt
    39661 ttgaacagaa agactttgta caaaaagtaa tcaaagaaga agaacaatct tttttacgca
    39721 ctcttgaaac aggtatcaga agatttgatc aatataacaa gtctgtaatt gatggagagt
    39781 ttgcttttga attgtacgac acttatggtt tccccattga ccttacgcaa ttaattgcac
    39841 gtgaacgaaa catgcaagtt gacatgaatg gatttaacca atgcttagaa caacaaaaag
    39901 aaagatccag agctgcatca gcagttgatg cagaagactg ggttattctt gaaaacagtg
    39961 agtcgaaatt catcggatat gacaatacaa cggctgaggt aatcattata aaatacagaa
    40021 aggtaaaatc aaaaaataaa gaacaatatc aacttgtctt caatcagact ccattttatg
    40081 cagaaagcgg tggtcaggtt ggtgatacag gctatatcca aaacgaaaag gagaaaattt
    40141 ttattactga cacacaaaaa gaaaataatc agatcattca cttgtcagat aaattacctg
    40201 ttgatttgca agcagatttt attgcaaccg tcaacagttc gagaaggaga aaaataacag
    40261 ccaatcacag tgcaacacat ttaatgcatg ctgcattaaa gcaagtttta ggcaatcatg
    40321 ttgtacaaaa aggttcctta gtaaatgaag agcatacacg atttgatttt gcacattttg
    40381 caaaagtgtc tgatgaagat ataactaata tagaacaatt ggtgaatgaa aaaattcgtg
    40441 agaacatagc tttagatgaa agaagaaatg tcccaataga agaagcaaaa tcaatgggag
    40501 caactgcact atttggcgag aaatatggag aatatgttcg tgtcattatt tttgatcctg
    40561 catacagtat ggagttatgt ggtggcacac atgttactgc aacaggcgaa attggcttgt
    40621 ttaaaatcat cagtgagtcg gctgtggctg caggcgtcag acgtattgag gcaataacat
    40681 caataaaggc tgaggaatac atcaacaatc aactgagaca attaaacaca attaaatcag
    40741 aactaaagaa tccaaaagat atagttgtaa gagtaaaaga gctgttgtct gaaaatgaaa
    40801 tactgcaaaa acaaatagaa agctatgttc atgagaaagc gcagcgcatt aaagcagaac
    40861 ttctaaaaaa agtaagatta cagaataaca tcaatgttat tgcgcagaaa attgacttag
    40921 ccaatgctga tgcaattaaa aacatttctt ttgagcttcg caatcagtta ccggactcct
    40981 ttattttatt ggcttctgaa agtgatggta aggccatgtt gtcattgatt atttcagatc
    41041 agttggtgca atcaggaaaa ctgaatgcaa ccacaattat cagaagtatt tctaaacaca
    41101 ttcaaggtgg tggtggcgga cagccgtttt atgccactgc cggtggtaaa aatcctgccg
    41161 gaattgatgc tgcaattgaa gaagcgttaa agatgatata aaaagagcga ggctgcccaa
    41221 atgggcagcc tcgctctttt taaaatattg tcagaatatt aatattccca catatcgtgt
    41281 tcaagattta taatctctga cttaattcgc tctgcttcaa gcaatgcatc taagccggtt
    41341 ttgtaatctt caatacgtct gttgtaaacg ttagattctt taataatgta gctattaaac
    41401 atgcgtttat gaaatacatc atcaaatgtt ctacgttcag tatcattttc acgattgaaa
    41461 gcttcagcat ttgcaagtaa ctttcttgct tcggggtaat aaatccaaaa aagaggcttt
    41521 tgaatattac cttctctgat atttccatat tcatcttcgg caagcattaa tggtgcaata
    41581 ccaataatac ggcactccat aactgagcgt tgcttatcaa agaaccactc ctctttaata
    41641 cgataggcaa taactttatc acgtgaaaag gtacgctgaa cggctgaatc acgagtaaga
    41701 tatggaggct ctggatctgt tatctgaata gtgtccattc ctgctccacc aattttctta
    41761 aactcttcaa gagtcatagg tgtggtgaat tcatcatctg ttgttgaata tgccgttaag
    41821 gatccttcat caatagcatt catcaaaact tctatcaaac tctttctgtc ctttgtatcg
    41881 ccttcacgtg ggaaacgaag tggtaaatta atcttttgtt tgagatctat tacttcccaa
    41941 acacgctttg cccataatac atcagcttcg cgcagaaatg catattccat tactttgcgc
    42001 gtaggattgt gttcttttac ataaacacca tccaataccg tttgtgcatt tgtttgtttg
    42061 acagtaaatg ccattgcaaa caggaaacaa aacaacagga cttttttcat cttaccctcc
    42121 gtggtttatt gttaaacgta ttattgaatt gtaaagttta cacttggaat acttcttgtt
    42181 cctccgtcag gaccaacagc cttaatatat tcaaaatata ctttatcacc ggcacgagct
    42241 ttacttaaag cagctttcat attagcagta ataaggttgt tattactttc taattcaatt
    42301 ggatctttac cttttggata taaagacatt ttgaatttcg ttactttata ataaatttcg
    42361 aagtcgaaat tttccaattc ggcaataact gcactttgag caatcagcgc atttttttgc
    42421 attggtcctt ctcttttgcc tgcaattttt gcaacaggat cgggaactct cttcacacgg
    42481 aatttcattg cgcccatgtt aattttcttt ccatccatat cagcaataat tgatattgtt
    42541 gcttcacctt gttgtgttac ttttacaata tagtcagcac cagttccttt tgggtttttt
    42601 gttaatgtac ctccgccacc tgaaatagaa gcaacaactt tattgtttgg tacaccaggt
    42661 actgaaatcg aaacagggtt ttccggtccg atatagaata cattcatctt tgttggagaa
    42721 acaacagctg ctggttttgc agcaatatat tctgcttcaa atgggtattc tttaacttta
    42781 gttggatcct cagggtcttt aaccttaatt actccacccc atttctttac accctctgat
    42841 gaaggacggt caacatattt acctaatcca ttttctacag gtaaggttct gcctccaaca
    42901 acgatttctg catcagaact tttattaaaa gcagctaaaa atacatctgc tttataatcc
    42961 tgaccggtta atacatagct tgttggtgca actaccttag gttctacatc agtaaacttc
    43021 aaatcttttg cattaatttg cgataaaaga tagttgttta cttgtgattc cgcagtttta
    43081 acatcggatt gaattttgct aagcatagct aatgcagcaa caacaggagt atggtaaaat
    43141 gtagccattt cccaggtttt cttcttttct tcctttgcta atttactatt gggatcagga
    43201 tccttcaaat taatcaactc atcaaaacgc gtttgaaaat cctttttgtc atcggcatca
    43261 acaaatccaa ttagtttaac tttatactct tctaacttat tctttaactc tgtaccttta
    43321 aagcctttac catcttgctt atcaccacac attactaaag ttggtgtatc atagtcatcg
    43381 tttttctgta tatcacgagg accggggatt ttcgcattgg cttcaagttt atccgctttt
    43441 ctaacgattt cattctttat gtcttcaacg tatttataca aatcatcaga agctttatga
    43501 acctgatttg ctttctcctg aaaaggtttt gcttttacag catcagcttg tgcagctgta
    43561 gcaaatgcat ccattacctg ttgattcttt ttaataacgt taaggttgga tgcagttatt
    43621 ccttcatcaa tggtaataaa tgcattgatg atttcttttg aaacgttaag agcaagcaga
    43681 gccgttaaca cgaggtacat catgttaatc atcttctgcc gcggtgtcag tttaccactc
    43741 gccatagtct atttctctat atctaaaatt ggttggttat aaaataattt atttatcaga
    43801 ttacttgttt ggattcaggt tcattgctga aagcatgttt ccataaacag tatttagaga
    43861 agacaggttt ttagccagtt tagaaacttc atctttatac ttcttagtat catctaacga
    43921 ctcattcaaa tttgacatta actggttaat gccatcataa aacttggaag tctgttttaa
    43981 gtgattgttt gaatcttgca actgaagctc ataaacagca tttaatgcgc ctaggttttt
    44041 accagccatt tgaacttgct cgttatacat tttaacgtca tcgctggcag tagataaaga
    44101 actcattgcc tgtgcagcct tttgataaga accggaaagt tctgtaacag acttagtagc
    44161 tgcatttaca ttttttacat aatcatttgt agcaacagaa gcatcagaaa gatctgccat
    44221 tttagcggta ttatcgccta atgactttaa ccctgttcct aaacttgcaa ttaaatctgg
    44281 accaatttta gcatcagaca acattttatc taaagcttgt gataccggat caattttttt
    44341 ctttttatct cttgcattgt ttggatcatg catacctgct aattcaggat agacaagtgt
    44401 ccagtcaatt tcttcatgta acggttcaaa agcagagaag aagaaaatta cggcttcagt
    44461 aagaagacca actgtaagca tgataccagc accttcccag tgttggattt taaataatgc
    44521 tccagcaatt acaacagatg caccgagacc gtacaacatt gccatgaatt ttttgtactt
    44581 ttttccggat gtgaattcag ctaatcccat agttgatttt gaaaatttaa ttggttgatt
    44641 tttaatttat ttattttggt gataataatc gaaataattt attcttaaaa gtcagcttta
    44701 tcgcgaccca agaatgacat aacacaacgg aaacctacgt aacattttgc agtgtcctga
    44761 tactcataag aacgtgtgcc tgtctgtaaa aagtatccta catctttcca tgaaccgcca
    44821 cgaattactt ttctctttaa tgctacagga tcagagtctt ttgcatcgta agaataatcg
    44881 gggttaaggt catgagagaa attatatgca gcttcctcat aagcattgct tgtccattca
    44941 gacacgttac ctgacataca gtataagcca tagtcgtttg gccaataaga tgttgttttt
    45001 actgtgtaaa agccaccatc atccatatag ttaccacgca ttggcttaaa gtttccaagg
    45061 aaacaaccgc gactgtttct gatatatgga ccaccccaag gatatggagc taaatcatgt
    45121 ccgcctcttg ctgcatattc ccattctgat tctgtaggta atctaaaatc ctgaacaaat
    45181 gattctccgt tttcctgtaa gtaactattc aataattgtg ttctccagat acagaaagca
    45241 cgggcttgtt tccaagtaac acccactacc ggataatcat cataagccgg atgccaaaaa
    45301 tacatgtttg taacaggatc attatatgaa tatgtataat catgtatcca tgctaatgta
    45361 tcaggatata cattgataat atctttcttt ataaatactg aacggtcagt cataccttgt
    45421 tcacggtttg acttttgagc agcttctttt aaatcaatcc agtaatattc gaaattcagt
    45481 ttacgtgaat caatttcttt tcttctgtaa aagcgctcac tttccggtaa aaacaactca
    45541 gctaaggttt ctacgttctg ctcatcttcc catttaattt tcttacgcca attgatgcgc
    45601 tcgccatatt ctccttcttc gataagatga tcaccaccga ttaatcggtg cgcaagtgag
    45661 tcgcgaaccc actccacgaa ctgacgatac tcgttgttgg taatctcagt attgtccata
    45721 taaaatgcat gaacagaaac agatttgctc tgtgcaacca ttgcgtatgg tacatcctga
    45781 tcgcttggac ccatattaaa tgcgccagat ggtataaaca ccatgccgta tggatcagcc
    45841 tgataccagc gctctcggtt aagagcacca actaactgtc ctttgttacc gccaccgcag
    45901 ctgctgagta aaataactgc aatgatggga atgaatagtt ttctcatgtt tgccaattta
    45961 gtaactggag aattatggat gttaaaacga tttatattta tttttattac tttcagtctg
    46021 tactatgctg ttttattatt ctgtttatac ttcagtttca aagataagtt actaattaaa
    46081 ttaagtttcc cgtaaattat aaaaatctta catttcttaa tacaggaatt acttttggct
    46141 taatcacata acaataacct aacattattt catgtgttcc gctatgatac ccttttaatt
    46201 tagaggtagt aatatcataa gagtatccta atttcagatt ttcgataata tttataccag
    46261 ccattaaaac aatagcatcc tgaagtctgt atgatgctcc aatccaaact ttatctttaa
    46321 tgtgcagatt agcatttata tctgcctgtg tttctttagc atcagacttt ataaataaag
    46381 atggttttaa tttcaaagat ggggtaagat cgaagtcaac accacccata aaataaaaat
    46441 ggggtactac ttctactttg gcatcgtctg tatattcaat ttttccaccg gttaaatgtg
    46501 ttgccgaagc accaacataa aatttatctg atttataata taagcctgcg ccaaggtcaa
    46561 acttagagcc tgttcctgat tttaccaatg gatcatcagg gtcaatagca ttaaaatcat
    46621 tgccataacc acgctgtaat aaaccggcat caagtccaat tcctaatttg cctgaaccta
    46681 aatctaaatg atatgaatat gccaatttga tgtttagatt tttctggttc agtggcttgt
    46741 cagttaaaac tgaaagccct agtccgccat gtagaacttc aacaggtgat tctaccccaa
    46801 aaacagccgt tttgggccca cctccaaatt tatcccattg atcacgataa attaaagtac
    46861 cacaaattgc ttcgttggta cctgcagatg ccggatttgg atacaagcgg ttaaacatgt
    46921 tttgtgaaaa ttgtggatcc tgttgtccaa aggatactga tgcggttgcc acagctacga
    46981 ctgtaaaaag tctcttcata tactattcag gttgttgatt cgtttaatat tatacgtctt
    47041 aatttgcggc cctaaagtaa gcaaaaaaac aaattgcaaa caaaagacac tttttaattt
    47101 caataaatga gttaaaatcg ggcatagtta cgacagtttc tttaaagctt gccaccatct
    47161 ttccggaatt tcattgttac aagccttctg ataatcttca taactgcatg gaactaactg
    47221 atgcctgtct aatttctttt ttatcttact cagaggtatt tccatccacc agcgaccggt
    47281 tacattactt ttcagaaaat gcatctcctc attattctct ctaagtgtca ggttataaac
    47341 agtaaactgc gaacttgtac tatcaggaaa atcgttcttt ctattgctta caccttccat
    47401 aaaataccac accatttgcg caattaatgc tgcagtcatt tgatttgtat ctgctgaagg
    47461 tgcatactca tagattccga ttccttctaa tttatcactc atacctgcat aatacatgat
    47521 ttgacaggcc atatcacctg ttaaaccatt gggtgacgga gttgaagtag cagaggcatc
    47581 gctgtatctg atactcgata aatcaaaagt catcacatcg gcatttctta taatcggttc
    47641 catttcagta gcatcctgat taacctctcc cagtctgcat gtatcgaaat acaaattatc
    47701 catcatattt acagcatagt cacctacaaa ataggtctga tgtgcaatat tactgtaatt
    47761 atagagatga ttgggttgat ccatcacaat atttccaagc caacttgcag agtctaaagg
    47821 gtctgatggc atacctaaat caaaacgagc atctacacca accatattta taatctgatc
    47881 tatcttctta tatgctctgt aatgtgctac tgtcatatcc tgacttgcac caataattac
    47941 tggtataatt tttaattgaa gcagtgaaat gacaatctcg gttaaagcag cataggtatc
    48001 ctctacggtc acacctaatc tgacatttcc taaatctgcc acaggaacac caacaccatg
    48061 tttctttaag ctataaagcg actgcctaac ataatcaaca ccattcttag cttcatagtc
    48121 tctttttgca cctcgggtct cctctactcc aacaatagca attttaatat tattcataaa
    48181 aggaaatcct gatgacccaa gatttttttc aatatgatgc cacatagaat caactggagc
    48241 attagacatt ttctgctcaa cagactcact gataggactg aaaaattcac tgatctgcat
    48301 gactaccttc ctgacttagc tttacttttt gttttgcctg atgaggctgc cttctttttt
    48361 gttgaggctg atgatttttt tgcaggagat tttgcctttg tcttctttgc aacagatgac
    48421 ttttctgcct ttgctgcctt ttcttttttc aaaggaacgc cagacaattt tgcttcaatt
    48481 aactccccaa gataatcaat atttacatcg aaagggtcgt cacctttctt aagtgctaca
    48541 aacaaatcgt catatttaat ataagggcca aaccttcctt tggcaatgac tacatctttg
    48601 cctttatact gtcctgcatt tcggggcaga cgtggcttat ttaaaatatc tacaactgtt
    48661 tctaaatcta tttccaaagg atcttcacct ttgggaagtg atataaattc tactccatat
    48721 ttaatgtacg gcccaaatct tcctttgtta attacaatat caagtccttt gtgttgtcct
    48781 aatgtacgtg gtaatcgagg tccgtcaaga atggctttta tggaatctaa ttcaatagta
    48841 aacgggtctt cttctttagg aagggaaata tattcttcac cgaatttaat ataaggtccg
    48901 tagcggccat tattaacgat taactccttg tttttatact gaccgaaatg acgaggcaat
    48961 ttaaataaat ccagaacttc ctctagagta actgtttcaa gtcgctggtt tttttgaaga
    49021 ctggcataca tcggtgtgtc cgactctttc tcagctacaa tttctgccat tggaccaaag
    49081 cgacctaaac gaacaataac ttttcttttt gtttttgggt ctttgcctaa tgttctttca
    49141 cctgtagctc tatcacttgt ttcaattgtg ttctctacat tcttatgaaa tggtttataa
    49201 aatcctgcaa tcattttatt ccatttcaac ttgccttcag caatttcatc aaattctttt
    49261 tcaatttcag cagtgaaatt ataatcaaga atatctttga aatattctac cagaaaatca
    49321 tttacaacaa tacctgtatc ggtaggatac attttatttt tttctgcacc ggtaatttct
    49381 gtcttttcaa ctttgtttat cttactgttg ctaagttcaa gcacagcata ttttcgttct
    49441 ttaccctgac gctcctcttt aattacgtat cctctttttt gaatagttga aattgtaggt
    49501 gcatacgttg aagggcgacc aatacctaac tcctctaact ttttcaccag acttgcctct
    49561 gtaaatctag ccggaggctg tgaaaaacgc tgtgtggcag taattgattt aaaatctaat
    49621 ttttgtcctt cttttagtgg tggtaataat gtgtcgttat cctcttcgtt ttcatcatct
    49681 gacgattctg tatatacttt tagaaaacca tcaaaaatga taacttcacc tgttgcaaca
    49741 aatttctcag tacttttatc agaagcaatt ttaacgatag tccgttctaa ttttgcatca
    49801 gccatttgtg aggcaattgc acgtttccaa atcaaatcat ataaacgctg ctcaccaggt
    49861 gttcctttta taaccgaacg gttgaaactt gtaggtctta tagcctcgtg tgcttcttgt
    49921 gccgatgccg acttggtttg atacttcctt ttctgatgat aatttttgcc ataagaagta
    49981 ttgatttcct tctctgcatt agcaagtgcc tgatccgata agtttactga gtcagtacgc
    50041 atataggtaa tcatccccga ctcataaagc ttttgagcaa tgctcatggt tgttgccact
    50101 gcatatccta actttcttga agcttcctgc tgtaaggtag aagtagtaaa cggtgcagca
    50161 ggttttcttg ctgaaggttt tttctctaat gatgcaatat taaaagtagc atttctattt
    50221 ttctcaagaa atgcttcagc ttccttttct gttccgaatt ttttatctaa ctctgctttc
    50281 aggatagttg tttttcccga tttgtcttta accagaaaat tggctactac cttataaaat
    50341 gaagcaactt caaaatcctg aatttcacgt tcacgctcaa ctattaaacg cactgctact
    50401 gactgcactc gaccggcaga caatgaaggc tttactttct tccataagat aggcgataat
    50461 tcaaaaccta ccaatctgtc aagaattcgt cttgcttgct gtgcatcaac aaggtgtata
    50521 tctattttac gtggattctg aatagcttca aggattgcat ctttggtgat ttcgtgaaaa
    50581 acaattcgtt ttgttgctgc atcttttaaa ttcaacacct cagaaagatg ccatgaaata
    50641 gcttctcctt cacggtcctc atctgttgcc aaccacacag tctttgcttt acgagataat
    50701 tttgtgagct cgtcaattac ttttgtttta tcctcactga cctcgtatat cggttcgaaa
    50761 ttattcttga tatcaatgcc attgttggct tttgccaggt cgcggacatg accataactt
    50821 gacttaacaa caaagtcctt accaaggaaa ccttctattg tttttgcctt tgccggtgac
    50881 tcaacaatga ctaaattttc tgccatctgt tactttttga agggcgcaaa ttaaatcatt
    50941 tcattttgtt atttccaaat gaaactatga ccaatcattt gccgacttgt ttattttaaa
    51001 gtacctttgc atcgttttac agagctaaaa ctataaaacc tgatcaggtt aaaaattgtt
    51061 gaaaacctct ttaaaacttt taaaacaaaa attttacaaa gaaaatgcac aacgacataa
    51121 aagtaattga tgaaagccgg caaggtgaac ctaccatgct gcaatcatca tcagaaaatc
    51181 tgagccctaa aaaactttat ttagaaagtt atggctgtca gatgaatttt gcagacagcg
    51241 agattgttgc atcaattttg aagaatgaag gttttgaaac cacaaaagat gttaatgaag
    51301 ctgatgcagt ttttattaat acctgcgcca ttcgtgacaa tgcagaacaa cgtgtccgaa
    51361 caaggcttaa agattttaaa aagataaaaa aagaaaatcc tgatttgatt gttggcgttc
    51421 ttggctgtat ggcagaaaga attaaatcgc aatttctgga agaggaaaaa ttagtggata
    51481 ttgtagttgg ccctgatgcc tatcgtgact taccactatt gattgagaaa gttgaagacg
    51541 gacaaaaggc tgttaatgta attttatcga aagaagaaac ttatgcagat atcactcctg
    51601 tcagattagg caacaatggt gtaactgcct ttgtgagtat tatgcgtggc tgcgacaata
    51661 tgtgttcttt ttgtgttgta ccatttacac gtggtcgtga acgttcacgc gacccacaaa
    51721 gtataattaa agaagtagaa caactgtata atgaaggtta cagagaagta acattattgg
    51781 gtcagaacgt tgactcctat ttatggaaag gtggtggact gaaaaaagat ttagatactg
    51841 aaactgttca tttggcatta aaccataaac taccgcccga gcagcaaaaa gattttgtca
    51901 ctttttctaa tcttcttgaa atggtggcag atgtgaatcc tgacatgcgt gtcagatttt
    51961 caacatcaaa tccccgcgat atgactgatg cagtattgca cagcatggct aagtacgaaa
    52021 acatttgtaa gtatatacat cttccagtac agtctggcag cagtagcgtt cttgaaaaaa
    52081 tgaacagagg atatacccga gaagattaca ttgcaagaat agaaagcata aaaagaatat
    52141 tgcccgaatg tggtatcagc acagatatta ttgctggttt ttgcagtgaa acagaagaag
    52201 aacatcagga aaccatcagc ctgatggaac agacaattta tgattttgca tttatgtttg
    52261 tttatagcga aagaccaggg acctttgcag aaagaaaatt tacagacgat gttttgcctg
    52321 aagttaaaac aaaaagattg aacgaaatag ttgcactaca acaaaaacat tcagcaatca
    52381 gaaccaatca atccatagga aaagtttata aagttttaat tgaaggtgtt tctaaaaaat
    52441 cggatgaaat gctcatgggc agaaactcgc aaaacacggt ggttgtattt ccaaaaaaca
    52501 atcacaaacc aggagagtat gttcatgtta aagttaataa gtgtacttca acaacattga
    52561 ttggtgaagt tgtataacaa atttcgaaaa ataaaaacaa caataaccat tcactgttta
    52621 agaaaaacac aagaagaaac acaaagttaa ctatcggaaa tgaccataca ggatataaaa
    52681 gcacgatttg gaattattgg tttttcatca gtactcgacc atgcattaaa cacagcaaga
    52741 caagtagcac ctacagattt aactgttttg attacaggtg agagtggttc cggaaaagag
    52801 gttttcccac aaatcataca tcagcttagt tccagaaaac acggcccata cattgcggta
    52861 aactgtggtg caatgcccga aggcactatt gacagtgaat tgtttggtca tgaaaagggc
    52921 gcatttaccg gtgctcatga aatgcgaaaa ggctattttg aagtatcgaa tggcggaaca
    52981 atctttttgg atgaggtagc tgaattaccc atgtttactc aggtacgttt attacgtgtg
    53041 cttgagtcgg gagagttttt aaaggttggt tcaagcaagg ttcagaaaac agatgttaga
    53101 gttgttgcag ctaccaatgt aaatattccg gaagccatta gcagaggtcg tttcagagag
    53161 gatttatatt acaggctcaa taccgttcca atacatgtgc ctgcactccg tgaacgaaca
    53221 gaggacgtgc atttattatt cagaaaattt gcaggagatt ttgcggaaaa gtatcgtatg
    53281 cctgcactta cacttcagcc cgaagcttta cgtttactga tggagtacag atggccggga
    53341 aatgtcaggc agttgaagaa tgttacagag cagcttagca tcattgaaca gaaaaaagaa
    53401 atcagtgcag aagtactttt aaaatacttg cctcaggaaa attttgcaag cgtacccact
    53461 ctattcaata atcacaaagg cggtgatttg aatgagcgtg agttgttgta taaagttctg
    53521 tttgaaatga aaaaagacat ttcagagctt aaaaaaattg tttttaatat catcagcaat
    53581 ggcgagcctg tgcatcttga aaattcagat attgatacag aaaggttttt gaatgatgta
    53641 aaagggcatc ctgtactaca cactaacgac acttcaatta cgctgacaaa gcccacacat
    53701 attgatgtta agccgcatga acatgttgaa gaatcgttga gcattgccga cagagaaaaa
    53761 gatttgatta tccgtgcttt ggaaaaatac agaggtaaaa gaaaaggtgc tgccaaagaa
    53821 ttggggattt ctgaacgaac actttacaga aaaataaacg aatacggcat taaagactga
    53881 tgagtaatat aaaaaagaat tcgggcatat taaggagtaa attattctca agattaattc
    53941 tgttaatggc ctttactgca atgatggaat cgtgtggtgt ctattctttt acaggagcat
    54001 caataacacc gggtgtcaaa actgtcagca ttcagtattt tcctaacagg gcatcagtag
    54061 cacagccatc attaagtcag gtttttactg ataagctcaa agaaaaattt gtttcacaga
    54121 cttcgctcat ccaggtcaat caatcgggtg atttacgttt tgaaggatat attagtgact
    54181 acaatgttca gccaactgca attcaaagta cagacgtggc tgcgttaaac cgcctcacca
    54241 ttactgttca tgttaaattt gaaaacgtga aggatgaaaa gcagaatttt gagactagtt
    54301 tttcgaggta tgccgacttt gattccagga aagatctgac aagtgttgag tcatctctga
    54361 tgaatgatat atcttcgcaa cttgtagatg atatttttaa taaatctgtt gttaactggt
    54421 aatgactaaa gaacgttttt ttaattttat tcaacaaccg tataatattc agaccaatga
    54481 cattgaagag ttggaacaaa tcattgagca gttcccttat tttcagactg cacgactgat
    54541 ttacaccaaa tcactacata atgaaaacag ttatttatac aatgacgagt taaaacgaac
    54601 tgctgccttt gctgctgaca gaagcgttct ttacaaactc atccacacca tcaataaaga
    54661 cacacatatt gttgactcag atagtttaga ttctgatgaa atagcatcaa gagttgaaat
    54721 tagtcctgtt caagaaacac ctgaagtgga tgcacttgta tttgaagaaa aaacagaagc
    54781 cgaaactcag gtaattattt ttgaagaaaa agataaatcg gatgcagatg atgaaccgat
    54841 attatttgaa gaaaagacat tagctaatga ggaggtcgag cctgttgttt ttgaagaaaa
    54901 gctacctgaa actacagcaa cagaggccat aatatttact gctattgaag aacccatcaa
    54961 taccaatata acagaggttg atgaaaatga gccttcaaac aaaaatgcag tgatagcgtc
    55021 agagaaaaaa gaacgtgccc tgacaccggc agaaattttg tcacaacgac tgcgtgaaat
    55081 cgaatcgcaa ttggaagaca aaaaggaaga agaaactgaa gcagtctctg agttgaaaga
    55141 agaaaaatct ctgtctgagc ccccaaaaat tacagaacca gtaaatattc aacctgaagt
    55201 attgcctgaa agcattgaaa ttaatccatc gctactggaa attaaagaag aagttgagat
    55261 tcctgaacct gaagaagtag aaaatttaat agccccggca ccagaaaaca cagaaaaaac
    55321 tgttgaagtt gttgtagcaa gtccggaaat taaatcctcc ataattgaag ataatgttac
    55381 tccagatgcc ataattcaac ctgaaataaa accattacca gaggttgata aaaaagaaaa
    55441 acattcattt tcagactggc tgcatctttt tcagccgcag gaggaaaaaa aaactttaat
    55501 tactaatgct ttagttgaat caaacagcga caaaaatctg attagtaata agttaactgg
    55561 agaagatata gctaaaaaca tcaatagtga aatcataaat caatttatcc aaaacgaccc
    55621 caggatagaa agtagcaaga ctaagttttt tagtccgggc aacttggcta agtccagtgt
    55681 tactgatgtt tccgacattg tttcagaaac tttggcaaaa atatatttag ggcaaaataa
    55741 tttcagtaag gcaattcaaa cttatgagaa gctcatattg aaatatccgg aaaaaagtgt
    55801 ttactttgca gcccttatta aagaaataaa gaaatcacag atttaacaat caatcaatat
    55861 gtacacttta attaccgtgt taattatttt aattagtgtt ttattatgcc ttgccgtatt
    55921 ggtacaaaac tcaaaaggtg gcggtttggc ttccggtttt tctgcaccac agcaaattat
    55981 gggcgttcgt aaaactactg actttcttga gaaatttacc tggggattgg gaactgcatt
    56041 aatcgttttc tgcgttatag caaattttat gactcccggt agtaataaca gcactgaagt
    56101 aagagcttca tctatacagg agcaaattga caatgcaaca ctaccagcat caccacaggc
    56161 accattgcct tcgcagcaaa atgcacaacc tgcacagcaa cctgctacac aaccggcaaa
    56221 ataatcttgt cagttaataa cttttaaagg tgccataatg ctgaaattat gacaccttta
    56281 ttatttatac caaagccaca aatcgcaaat ctgtcagtaa aatagtagtg gcacagaaac
    56341 tgataatggg caaactgaag ttatttataa aagtataaaa gtcaaacaat ttaaatcaca
    56401 ttattactat gagtaaaatt aatttaacac cactggcaga ccgcgttatt gtagaagtgg
    56461 ctgctgcaga agagaaaaca gcaagtggaa ttattattcc tgacacagca aaagaaaaac
    56521 ctcaaaaagg caaagtagta gctgttggtg gaggtaagaa agatgagcca atgactgtaa
    56581 aagttggtga cagcgtttta tatggaaaat atgcaggaac agaaatttct gttgatggca
    56641 aagaatatct tatcatgcgc gaatcagaca tcttcgcaat tgtttaaaag cgataaaaac
    56701 aaaaagaaag aaagaaataa ataaataaat aaacaactta aataaaatac gaaaaatggc
    56761 aaaagaaatc acatttaacc ttgatgcacg cgatgctctt aaaaaaggtg ttgatgcatt
    56821 ggcaaatgca gtaaaagtaa ctttaggacc taagggccgt aatgttatta ttgataaaaa
    56881 attcggttca ccaacaatta caaaagatgg cgttactgtt gcgaaagaaa tagaacttac
    56941 caatagcatt gagaatatgg gtgctcagat gttgaaagaa gtagcgtcta aaactgctga
    57001 catagcaggt gacggtacaa caacagcaac agtacttgca cagtcaattg taaacacagg
    57061 tttaaaaaac gttactgccg gtgccaatcc aatggattta aagcgcggta ttgacaaagc
    57121 tgttgaagca gtagttgcat cattaaagaa aatgagcaaa gaagttggtg acgacaataa
    57181 gaaaattgag caggtagcta caatttctgc aaataacgac agcgaaatcg gaaaacttat
    57241 tgcagaagca atggctaaag taaaaagaga aggtgttatc actgttgaag aagcaaaagg
    57301 tactgaaact acagttgaga ttgtagaagg tatgcaattt gacagaggat atatctctcc
    57361 atattttgtt accaatgccg ataaaatgca ggcagtttta gaaagcccat acattttatt
    57421 gtatgacaaa aaagtatcta atatgaaaga attacttcct attttagaga aagtagttca
    57481 gacaggaaaa cctttgttga ttattgctga agatgtagat ggtgaagctt tagctacatt
    57541 ggtagtaaac aaaatccgtg gctcactgaa aattgcagct gtaaaatctc caggctttgg
    57601 cgatcgcaga aaagcaatga tggaagatat tgcaatcctt accggtggta cattaatcgc
    57661 tgaagagcaa ggattcaaat tagaaaatgc tgacctttct tatttaggca catgcgaaaa
    57721 aatcactatt gacaaagaca acacaacaat tgttggtggt gcaggaaaaa aggcagacat
    57781 cactgctcgt gttaatcaaa ttaaagcaca gatagaaact acaacatctg actacgacaa
    57841 agaaaaacta caggagcgtt tggcaaaatt agcaggtggt gtagcagtac tttatgttgg
    57901 tgcggctaca gaagtagaaa tgaaagagaa gaaagaccgt gttgacgatg cattgcatgc
    57961 tacacgtgca gcagttgaag aaggtattgt tgcaggtggt ggtgttgcat acattcgtgc
    58021 tataacagcc ttagacaaac ttaaaggtga taatgatgat gaaacaacag gaattgcaat
    58081 catcaaacgt tctcttgaag agccattgcg tcagattgtg agcaatgccg gaattgaagg
    58141 aagcattgta gtacaaaaag taaaagaagg caaagacgac tttggttaca atgcccgttc
    58201 agataagtat gaaaacatgc atgctgccgg agttattgat cctactaaag taacgcgtgt
    58261 tgctcttgaa aatgcagctt ctgttgccgg aatgttactc accacagagt gtgtgcttgc
    58321 cgacataaaa gaagacaagc ctgcaatgcc tgcaatgccg ggtggaatgg gcggaatgga
    58381 ttattaatat caaaatgaac gttgaaaatt aagaattcaa acattctcta aaacaaaaat
    58441 cccggtgcag acttgtaccg ggatttttgt tttttatctt atatttgttt caaatttaaa
    58501 aattatgaaa aaaagtttac tcgtacttat ctcatgttta tttgtaaata caaatattca
    58561 tgcacaaagc aacaccacaa acattgatga gataaatgct aatcttaaaa gcaatgaaat
    58621 tgtttatacc caaaaatatc aggcaatttt taatcagcca aaaatcacaa gtgttttaac
    58681 gggatgtgat tctataacaa ctacctattc ctctaataat ggacttgccg gagccatgtt
    58741 tgatgttatt gcagacagca atttagtaat aaatggaatc tacaccaacc tgtctgtagc
    58801 ttcgggtgtt tttgagatat attacaaata tggttcaagg gttttatatc tgcaaaaccc
    58861 tacagcctgg acactattag gaagtgccag tgtagttagt gcaggtattg acgtgccaac
    58921 ttttattccg attgctatga atattaccat gaacgctggt gacactgttg cattttatgt
    58981 aaccaacacc aacggtggtt caagcggatt aaaatatact aatggtgttg gctccggaac
    59041 cttgtatact gataatgggg cattgaaaat atttgagggt aatggcatca cctatccatt
    59101 caacagcctg tttgctgacc gtgtatggaa tggaactata aactattgta caaacactac
    59161 tggagtaact aatctgaaga atgacaacat tactatttat ccaaatccgg caagcgatta
    59221 tataaagttt attttcgaga acaataatct aaaaacatta atcattaaat ctttggatgg
    59281 caaaaaaatg tttgaagcca ttaatatcaa tacctcagat tatattttta aaagaaacgg
    59341 tcttgcaaaa ggtatttatt tttatcagat atattcaaac gacattgagg ttgcaaaagg
    59401 caaactcata ctcaactaaa attgccattg aataagtcgg cagaaaaacg gtttattatt
    59461 aaccctggta attgtttcta taatatcctt ttatgaaagt gcgctctaat atggcaggtg
    59521 caagtagttc aaaacagtta gcctgattta ttattctttt tagtgtcaat tccggaatat
    59581 caacacgtta gtgccaggca ttgagtgctg tagaaccgga tttagacaat tgctgaatga
    59641 tttttcagtt gtattgctat atcattataa gcagtaaaag tatttccttg ctttgcaatt
    59701 attctaacaa atggagccaa gtctttgttg tgttgagcaa ttgtttgttc aataatttag
    59761 cgttgttgaa agctttgttt tgaaagctgt catttctttt ttttaagaat aagatagtgt
    59821 aaccgccaac tcagggttac aagcttttct actcccctgt tgttagaaat acttttcatt
    59881 ctatctttca gtaattgaca aacaagaaaa gtcccggctt tttgagtcgg gacttttatt
    59941 aaaatctatt ttggaaataa attcttattc agtagatacc ttcaatattt tctttccttc
    60001 aaatacataa atgctttttc catctaaaga aacaattgat tttgaatttt tacgtgagtc
    60061 gaaaaatttt gctttacctg tattggcatc aacagaaaag taatcgcctt tttctgtatc
    60121 aaagaaaata ttctctccaa aaatcccgga aaactctccc tttttagttt tcactgccca
    60181 cacttttgca cctgtggcaa tgttgtggga agaaacaccc ttctctccta aaacaattac
    60241 attgtcttta ctaatattgt cacctaactc agaaggatta acaatttttt ctgccagtcc
    60301 aatgtcatca tcacctaaag gaatttcgta agcctcttta cctgttttgt aatctaaaga
    60361 gtataatgcc ttaccacttg acactacaat attattaccg cttgggaaaa tattggtaat
    60421 tccttttcta aagcgctcag agtcccatga cttcgtacca tccgttgtac taaatgcctg
    60481 tacttcgtaa ggttttacat tttgatattt tatcgttctg aaatattgtg tccattgtac
    60541 atacccatta ccataagttt gttttgatat ggcttgtact tctacagcac caccaatttg
    60601 cagaataatt ttatcttcaa ttttatataa accgggaatt actcttgcat cctttatttc
    60661 aggagaagtc caaacgagtt tgccggagtt gatttcatat ttttttaagt attgactttt
    60721 tctgcttttt acatctaaaa tgtaaacgta ttctccatca tataatggtt gtgctattgc
    60781 ctcatacact ccgtatctta caacttgtcc gtaaactttt ggtttaacta attctcttgc
    60841 aaaagttaca tcgtaagctg ctgcccaaat cggacttcct gttttatatt cataaacctg
    60901 taagccattc aattgtaaaa ataacttatc gcctcttaaa aatatcttgg caaccgattc
    60961 gcgggtaacg atttttcttt caacaattcc aacaaaagtt tgctcccata aaatatcgcc
    61021 attcttagca ttgattttta atatctgatt tctaaatcct ttaaacattg actgccatgc
    61081 atttcttggc atactaatga taacaattgc attctctttc tcatcataaa agtacttacc
    61141 aaccggtgtg ctaaatttaa ctgttgacca aagttcctct cctgttctgg cttttaccat
    61201 tgttaaatta gttttggtta cgattgcata agcattcaat tctgcaatgt aaataacctc
    61261 atccggatca tcaatgcctt gatatttatt ggtgctccac agttttacac ctgtttccat
    61321 atcaacaact accatttggt ctttacccgc ttttttatca aataaaaaca ctgcgtttga
    61381 ttcgtacatt ggaatctgca aatctacttt tccaatttgc tcatcaattt ctttaaactt
    61441 tttactccat tttactttgc cgtcaacagt ggacaccatg gtaaacattt tttcgttact
    61501 accatgcact attgcttttt cttcactcaa accggtttct gaaaatggat gcgtggcctc
    61561 tatttcccac gtagtcttca tttcatcgta cttctgagcg tagctcatca tacatgtcag
    61621 gcctaaagtc aggccggtta aaattagatt tttcatttta ttattgtttg tttaattatt
    61681 attaatttat ctgtttgtta aatgctatta tcaatattaa aggtgtatct gaatttcaaa
    61741 cgctgattcg gtgaaacaac aatgtcttta aatttatatt tcaacaacaa atccttcaga
    61801 gcattttgtt tagggatgtc ggtcttatcg tccgactcag caaagatggt aacaatcact
    61861 cctttttttt gtatggttaa atcaaataag taaataccct ttatgctctg ttttataatg
    61921 ttttttgcta aatcgctctc aggattttct aagtctgtat taagctgact aattgccttt
    61981 gactgaaggg agtcgttatc ttgtccttca atatagtctg tatattcttt taaagtttca
    62041 taattccatt ctataagctt tgtaccattt gataggtagg caacgggc
//
LOCUS       DAOQNP010000006        57898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_141_length_57898_cov_9.132445, whole genome shotgun sequence.
ACCESSION   DAOQNP010000006 DAOQNP010000000
VERSION     DAOQNP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 57898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57898
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141_length_57898_cov_9.132445"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..997
                     /locus_tag="PKY09_01635"
     CDS             101..997
                     /locus_tag="PKY09_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB11583.1"
                     /translation="MKRRITYIVLTILALTACNENKKEATNNSMTTSVTEKATMKSGF
                     SEVNGIKMYYEIYGQGKPLFLLHGGGSTIQTTFGRVIPLFSKDRQLICVELQAHGRTG
                     DRNTPISFEQDADDVAALLKNLNVHKADIFGFSNGGNTALQVAIRHPEVCNKVVAGSV
                     LLKRNGTFPQFWEFMKNGTFDQMPQQYKDAFIKVNPDSTKLITMYQKCADRVINLKDF
                     TDEQIKSIKAPVLLINGDQDVATAEHIVAMSKLIPNCKLAIIPGGHGAYIGEITTLNA
                     DYKESEFIIPLIEKFLDEKTKQ"
     gene            complement(1250..1711)
                     /locus_tag="PKY09_01640"
                     /pseudo
     CDS             complement(1250..1711)
                     /locus_tag="PKY09_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1757..1945)
                     /locus_tag="PKY09_01645"
     CDS             complement(1757..1945)
                     /locus_tag="PKY09_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11584.1"
                     /translation="MPQIQGVNREQITFSNLESQITKDNEIRFIDAFLDKLDLKELGI
                     QTLVQSEKKKAGNTYSCT"
     gene            2145..2366
                     /locus_tag="PKY09_01650"
     CDS             2145..2366
                     /locus_tag="PKY09_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HNB11585.1"
                     /translation="MYKPIEINRENLTIMGVKFPNIRSLESTANALGTNMFEGFEPTK
                     ELIQLYLDWKNGEIIETDFLSRLYSIYGK"
     gene            2356..2949
                     /locus_tag="PKY09_01655"
     CDS             2356..2949
                     /locus_tag="PKY09_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNB11586.1"
                     /translation="MENSYRYIDPDFKYTNKNGVLYNLANIENEDVLAVFESLKVSKR
                     LEELYENPIQIIDSESLIYIHRYLFQDVYEWAGKTRTVNISKDGKPFFEGERFETGFK
                     YVNSLITDFKNIEINDKRKIARTLAEILDNVNFLHPFREGNGRTQREFLRLLALEKGF
                     TLNLNPPDNKEIFERYMNGTINSDVEVLSHLIFEIIN"
     gene            complement(3099..3764)
                     /locus_tag="PKY09_01660"
     CDS             complement(3099..3764)
                     /locus_tag="PKY09_01660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB11587.1"
                     /translation="MTEFVPSLRFHFLTPFYDFLFGILLPEKKLRQTMIDMVEKLNPQ
                     NIIELGCSTGGLTLPLAKKFTTVPIYAVDADKRALSILEGKLNKISQNKIMLIHASSS
                     LLPFENKNAHTVISSLLFCNLLPDEKLKTLHEINRILTNEGHLLIMDWGNPKTILSTI
                     GFFILQTVGGRKTTDDLKKGIFTNLLKQNQFIIQKKESTTTLFGTLYFYQCSMQKGNS
                     TAR"
     gene            complement(3776..4522)
                     /gene="fabG"
                     /locus_tag="PKY09_01665"
     CDS             complement(3776..4522)
                     /gene="fabG"
                     /locus_tag="PKY09_01665"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015782609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNB11588.1"
                     /translation="MKLLENKVVLITGASRGIGRGIALTLAQHGAHIAFTYLSSPEKG
                     MELENTLKSHGVKAKGYQSDASQFNASEQLINQVLADFEKIDILVNNAGITRDNLLMR
                     MTEEQFDEVLRTNLKSCFNTTKHIQKSMLKQRSGSIINITSVVGEKGNAGQANYAASK
                     AGMIGFTKSVALELGSRNIRCNAIAPGFIETEMTGALDPATVKGWTDVIPMKRGGTVD
                     DIANAVLFFASDLSTYITGQVLHVNGGMYT"
     gene            4595..5311
                     /locus_tag="PKY09_01670"
     CDS             4595..5311
                     /locus_tag="PKY09_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB11589.1"
                     /translation="MKKLLFSVALVAMALTVNAQKFKIGLGGGFNSTWLMNKNVFDAD
                     DDLNVAMSFGGQFGLKTEYYFNDKLALELGIMRAGHNQKYDLNDGNDQLTLKLRYLDV
                     PLLLRLGGDKGGYFEFGPQFSFLNSAKDVDDDGDSRNVKGSLKGANIGLVIGFGVDVD
                     LSEQFTLTAGMRLGYGFSDVTKKYSNAIDMAQDMDDMSGATYWANFKDGTSGDFSYKT
                     TSRVYGGIHLGVLYTIPTGK"
     gene            5422..7248
                     /gene="asnB"
                     /locus_tag="PKY09_01675"
     CDS             5422..7248
                     /gene="asnB"
                     /locus_tag="PKY09_01675"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB11590.1"
                     /translation="MCGIAGFVDDSGDGEKTLSAMLKAIAHRGPDASKIFINQNVHLG
                     HNRLSIIDLSSDADQPMHDSGLTIVYNGEIYNYIELRTELERNGEIFKTKSDTEVILK
                     AYQQWGEKCVEHFVGMWAFALWDKNENKLFCSRDRFGIKPFYYINNNNKFYFGSEYKA
                     LKPTPVFSNEINQYQVLRGLQMGWICYEDETYFDVIKALPAAHSLVYYRDKAEIKIYR
                     YWSLQTGIYSNLSFDEKCATFYELFSQSVKLHYRSDVPVASCLSGGLDSSAIVSMVQE
                     QHPGMTYKTFSIYYEGKGDVDERPFVNEVLKKYPSLKPYYFSPSDSQVTEHFHHALYH
                     ADVPSTGSSFISQYFLMKLIKEQGIKVVLDGQGADEYLAGYKHTFYRMVADVLSSGQV
                     SQALALTGIVSKNQALSSKQQFAHLGKSLLSVAKSEQKLYTLEYLKYYPFMGKDKHIP
                     FWLDKVEGNRTDNFLYHLLFTTSLPSLLQYEDRNSMAFSIESRVPFLDHRLVEFSFGL
                     RNEEKANGIYTKWIMRSALQHVLPPKIAWRKDKKGFVTPGEVKWLRGPLTHLIESDFS
                     KLDFLNQNKIKRIIANYKHGDNSHALLVWRLCTLAYWQKYFC"
     gene            complement(7252..8037)
                     /locus_tag="PKY09_01680"
     CDS             complement(7252..8037)
                     /locus_tag="PKY09_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019050684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB11591.1"
                     /translation="MTLKVRTMADFLAANESPDILFWVGCAGSFDQRAQKITKAVAKI
                     LNSCNISFAVLGTEESCNGDPAKRAGNEFLFQMQALMNIQIMNGYNVTKIVTACPHCF
                     NTLKNEYPQLGGHYEVVHHSMLLQQLINDGKLIINGGKFLGKKIVFHDPCYLGRGNDV
                     YEAPRDVIKKLDAELFEMKRSRANGFCCGAGGAQMFKEPENGTKDINIERTEEILEAK
                     PDYVAVGCPFCMTMLTDGVKNFNKEDSVKVLDIAELIEKANDL"
     gene            complement(8055..9359)
                     /locus_tag="PKY09_01685"
     CDS             complement(8055..9359)
                     /locus_tag="PKY09_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNB11592.1"
                     /translation="MIQGIIFTIVLATGIFFFGKSIRRIRRNIFLGKPLDRSDNSSQR
                     WMTMIRVALGQSKMTVRPVAAFFHLIVYAGFIIINIEVLEIMIDGIFGTHRILSFMGG
                     FYDFLIGSFEILAVLVIIACIVFLARRNILKLKRFWSKEMTSWPRSDANIILITEIIL
                     MSLFLTMNACDYLLQQSGAAHYIKAGSFPVSSSLSNLFIGMPDSTLIFIERLCWWAHI
                     IGILAFMNYLPFSKHFHILMAFPNTYYSNLNPKGRLTNLESVTNEVKLMMDPSLPPPP
                     PSDAPMRFGARDVRDLSWKQLMDAYTCTECGRCTSVCPANITGKLLSPRKIMMDTRDR
                     LDEVGKNIDKFGMDHDDNKALLNDYISPEELWACTTCNACTEACPVNIDPLSIIIDMR
                     RYLVMEQSQAPAELNGMFTKIENNQAPWQFSPADRLNWKNED"
     gene            9437..11689
                     /locus_tag="PKY09_01690"
     CDS             9437..11689
                     /locus_tag="PKY09_01690"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11593.1"
                     /translation="MKNSLLFLLIYFFPSFYFYSESLCFDGNAMAMPTERADSLHIKL
                     ARAKEDKTKVLIYCDLMAEYIDNNLDTAFMFLNPALQLAEKIRWDEGIARVKIEGGRL
                     FWRKGSFETALKYHNDALAYFIKENNKDSIAEIITFIGQDYADWGKYQEALEYFVKAD
                     SIYRQTGNEKKLGGIYLLYSFIYINQGNYTEALKVNYKALKIFEANHDQYGAAITLMN
                     IADSYKSLGNYQDALKSYFNGVKIFEKDIDKFNLAETYSSIAEIYILIGNYQEALKYN
                     RKALAIAKGVNNGYLIASATQSIGEVYFQQQNYKEALGYFITSAESYRSVSDYLDLSL
                     ILSKVGLCQVKLENYEGAKKNLDDAYSIAQTLDSRLTLTAYYQGAELLDSATGNWQGA
                     YNNFKEYISNRDSSVNLENTKKILQQQIQYASDKKDALLQAEQDKKDAVQKVFRYSML
                     AGALTLLVLLIVVYNRYQIKTKTNAKLQEAYDDLQKTQLQLVQQEKMASLGALTAGIA
                     HEIKNPLNFVNNYSEVSHELIDEFLGSKDEKEREEIAQILKQNLEKIAEQGKRADSIV
                     KNMLKHSRTGSTEKQLTDINLICDEYLDLAFHGMRANTLGFRCALVKNFDPDLPKVNC
                     VGQDISRVILNLINNAFDEVYECDQLGLKGGDEYQPTITVTTAVSSQLTNKSGTKYVT
                     ITVGDNGRGVPDHVRQRIFEPFFTTKPTGKGTGLGLSLSYEIIESHGGKIDIDSKAGE
                     GTNFVISLPC"
     gene            11883..13097
                     /locus_tag="PKY09_01695"
     CDS             11883..13097
                     /locus_tag="PKY09_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB11594.1"
                     /translation="MKNIKIGILREEKVPHDKRVPFSPEQCKLIKSEYPGVEVVIQPC
                     SYRSFRDDEYRREGVVLQEDLSDCDILIGIKEVPKDKLIAGKKYLYFSHTIKKQPHNR
                     EMLKKIIRDKITLVDYECLVDDHENRIIGFGRYAGIVGAYNGIMAYGLKYGLFNLKAA
                     HQSFGIEELHKELDSIKLPNIKIVITGGGRVANGAIETMGMLNIRKVTPFEFLNYSFR
                     EPVYVQLHSENYYERKDGSDFSKADFYHYPEKYNCTFCDPGSYSSFTDLLVHCAYWNP
                     NAAVLFTKEDMRSPAFRISVIADVTCDIDGSIPSTHRATTIEDKFYGYNPVNEKEEEA
                     FSTDTITVMSIDNLPCELPRDASEGFGKHLIERVIPLLIGHDKDNVIYRATIARNGEL
                     TPRFAYLTDYIS"
     gene            complement(13101..13748)
                     /gene="rpe"
                     /locus_tag="PKY09_01700"
     CDS             complement(13101..13748)
                     /gene="rpe"
                     /locus_tag="PKY09_01700"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNB11595.1"
                     /translation="MHLIAPSILSADFSQLSSDVAMINKSNADWFHVDVMDGVFVPNI
                     SFGFPVIKAIKKYAEKPLDVHLMIVKPERYVEQFREAGADVLTVHIEASTHLHRTLQQ
                     IKKEGMKAGIAINPHTPVASLSDIVFEADLICMMSVNPGFGGQKFIENTYRKIQELKE
                     LIAIHNSKTLIEIDGGVDLNNYKKLIAAGADVLVAGNTVFSSSDPKATIEVLKKG"
     gene            complement(13841..14227)
                     /gene="apaG"
                     /locus_tag="PKY09_01705"
     CDS             complement(13841..14227)
                     /gene="apaG"
                     /locus_tag="PKY09_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNB11596.1"
                     /translation="MSTEVTAGIKVTVETFFQPTHSNPVQHHYVFAYRITISNESEES
                     IKLKRRHWYIIDSDNARREVEGEGVVGEQPVIEPGQLYRYVSGCNLNTEIGKMYGTYL
                     MERLSDNKMFYVKIPEFRMIVPSKMN"
     gene            complement(14512..14988)
                     /locus_tag="PKY09_01710"
     CDS             complement(14512..14988)
                     /locus_tag="PKY09_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11597.1"
                     /translation="MISTTFKLIKSAGATVLILLFAGMAMAQPGQKKIEKREKAEAMK
                     IGFITNRLNLTQDEAKKFWPVYNQFQDELSSLRQKRRGNRRDARQDFDQLSDKETEKV
                     VDDEILFRQSELDILKKYHAQFKQVLPIKKVAMLYQAEEDFKKELLRQLKDRRDAR"
     gene            complement(14969..15403)
                     /locus_tag="PKY09_01715"
     CDS             complement(14969..15403)
                     /locus_tag="PKY09_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11598.1"
                     /translation="MKDEIYNELKDKAPLLSSIEKKHLFDIPENYFDQLPSTIQQKIK
                     QPKTNIIKTLLSGLLKPQFAVGLIVILLVGSLYMFNQHKADNQYAFVAVNVDSLNVNE
                     LSDLLDTDQLESIVPADNNADAETINYLIENYNDLSYDINNI"
     gene            complement(15434..15979)
                     /locus_tag="PKY09_01720"
     CDS             complement(15434..15979)
                     /locus_tag="PKY09_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB11599.1"
                     /translation="MNHYSDKELLEQFSNGNADYAFNLIVRKYQQKIYTHVRRLVADH
                     NDADDLTQTTFIKAWKALGNFRADAQLYTWLYRIATNECYTFLKNKRKRYFIPIHDVE
                     AELADKLEHEPSLKANELELKLQKAILSLPEKQRVVFNLRYYDEMKYEDMSEVLGTSV
                     GALKASYHHAAQKIEKFLQSF"
     gene            complement(16044..17012)
                     /locus_tag="PKY09_01725"
     CDS             complement(16044..17012)
                     /locus_tag="PKY09_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HNB11600.1"
                     /translation="MKKFTYTEKKDTRSGFGAGLLEAGKSNPNIVALCADLTGSLKMD
                     AFEKAFPDRFFQCGIAEANMMGIAAGMTIGGKIPFTGTFANFSTGRVYDQIRQSIAYS
                     GKNVKICASHAGLTLGEDGATHQILEDIGLMKMLPGMTVINPCDYNQTKAATIAIAKH
                     IGPVYLRFGRPSWPIFIPENTPFEIGKALLLNEGSDVSIFATGHLVWKAIEAGMALAD
                     MGISAEIINIHTIKPLDEKAVIQSVSKTGCAITAEEHQINGGLGDSICQLLSRHLPSP
                     VEMVGVKDTFGESGTPDALMTKYGLDSKNIVFAVEQVLQRKKELAG"
     gene            complement(17020..18420)
                     /locus_tag="PKY09_01730"
     CDS             complement(17020..18420)
                     /locus_tag="PKY09_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB11601.1"
                     /translation="MLVIKSCVHNFRKLFFLIAFFIVFTSTVKAQKNNSLPLTRIEFL
                     FDASQSMYAQWQTNTRFEIAKKLLSEMVDSLDKITNVEMALRVYGHTKKFPPQDCDDT
                     RLEVPFGKNTAYLIKKRLSEISPSGTTPIAQSLNQCGNDFPADPARNIIILITDGIEE
                     CNGDPCAVSLMLQQKGIILKPFVIGLGLSKEYKDVFNCVGNYYDAVDETGFRTVFNVV
                     ISQALNNTTIQVNLLDEKHRPTETNVNMTFYDTYSGALRYNFVHTLNAKGLPDTLLID
                     PLGTYDIVVNTIPPVRKDSNRIVAGKHNTIGIDCPQGDLFLKFDGTSDYKNLQCIVRK
                     KGDSKTLHVQAFNTTTRYLTGDYDLEVLCLPRMLINDVNIAQSKTTTVQIPNPGMATF
                     QTVSAGYGSVYQEKDNKLNWVCNLNENNTHESILLQPGNYRVVFRPKISKEAIYTVEK
                     TFRITSGSGITVNINK"
     gene            complement(18425..19270)
                     /locus_tag="PKY09_01735"
     CDS             complement(18425..19270)
                     /locus_tag="PKY09_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNB11602.1"
                     /translation="MPTTEQLIKISTQVRRDIVRMVHAHQSGHPGGSLGCADFLTALY
                     FVIMKHNPTAFSMDGKNEDIFFLSNGHISPLWYSVLARSGYFDKKELSTFRSINSRLQ
                     GHPTTHEGLPGVRMSSGSLGQGLSVAIGAAQSKKLNNDSSIVYSLHGDGELQEGQIWE
                     AMMYAAAKKIDNLIATVDYNGQQIDGAVNDVLSLGNLKAKWEAFGWDVLQMNGNDMKQ
                     VVETLQNALKHTGKGKPVVILMTTVMGYGVDFMMGSHKWHGIAPNDEQLKQALAQLPE
                     TIGDY"
     gene            complement(19493..19618)
                     /locus_tag="PKY09_01740"
     CDS             complement(19493..19618)
                     /locus_tag="PKY09_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11603.1"
                     /translation="MGHHHVEEKGQTFLYEGGTGAFTAVWMILVFAFLFFLIYLG"
     gene            19824..20612
                     /locus_tag="PKY09_01745"
     CDS             19824..20612
                     /locus_tag="PKY09_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNB11604.1"
                     /translation="MSSNSVLSLFKSSIGKKVIMALTGLFLSIFLVVHVSGNFQLFKT
                     DSGLSFNQYSVLMTTNPFIKTISYLLYITIVLHAIRGIMLVLENKKARPVSYAMYDGK
                     ANSKWASRNMGLLGTVILFFIVIHLNDFWYQYKFGHIPFTMYKTELTTGKLVEVKEME
                     AGFKMGSKMEEMIVGDHKYVIVKDLYKEVKDEFKEWWMVLIYVVSMAAVSFHLYHGFK
                     SSFQSLGLNHRKYNGLIQSIGTWVFAIIIPASFAAMPLYFFINR"
     gene            20628..22637
                     /locus_tag="PKY09_01750"
     CDS             20628..22637
                     /locus_tag="PKY09_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNB11605.1"
                     /translation="MTNNILDAHIPAGDIEKKWSKYRSTVPLINPANKRNLEIIVIGS
                     GLAGASAAASLAELGYKVKVFCFQDSARRAHSIAAQGGINAAKNYQNDGDSIYRLFYD
                     TVKGGDYRSREANVYRLAEVSGNIIDQCVAQGVPFAREYGGLLSNRSFGGVQVQRTFY
                     AAGQTGQQLLLGAYSALMRQVGMGAVTMYSRHEMLDIVKIDGKCRGIIARNLITGELE
                     RHFGHAVLMCSGGYGNVFYLSTNAMGSNVTAAWKAHKNGAYFANPCFTQIHPTCIPVS
                     GEHQSKLTLMSESLRNDGRIWVPKKKEDAEAIRAGKLNPNDLKEEERDYYLERRYPAF
                     GNLVPRDVASRAAKERCDAGFGVNATGEAVYLDFAYNMKYKYGKMEATMNSIENPTEE
                     QLFDLGKAVVKEKYGNLFDMYKQITGVDPYHNPMMIYPAVHYTMGGLWVDYNLMTTVP
                     GLYALGEANFSDHGANRLGASALMQGLADGYFVIPYTIGAYLSSEIRTKAISTDHEEF
                     VAAENRVRETLQRLMQIKGTKSVDHFHRQLGKIMWEKCGMARNAIALKEAITEIQELR
                     KQFWADVRIPGEMNEFNPELEKAGRVADFLELAELMCMDALQRNESCGGHFREEHQTE
                     EGEALRDDDNFAFAAAWEMTGDAQWKMNKEELKFENIKIAQRSYK"
     gene            22671..23411
                     /locus_tag="PKY09_01755"
     CDS             22671..23411
                     /locus_tag="PKY09_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNB11606.1"
                     /translation="MNITLKVWKQRNKDERGRFETYQVQDVSEDMSFLEMFDVLNERL
                     INEGKDPVAFDHDCREGICGACSMYINGRPHGPLQGVTTCQLHMRSFKDGETIVVEPW
                     RSAAFPVIKDLAVDRSSFDRIMAAGGFVSVNTGNAQDANSILIPKDDADKAFEAAACI
                     GCGACVAACKNSSAMLFVSAKVSQLSLLPQGKAERHERVMKMVAQMDEEGFGNCTNTG
                     ACEAECPKEISIENIARMNREYLSAKIS"
     gene            23522..24232
                     /locus_tag="PKY09_01760"
     CDS             23522..24232
                     /locus_tag="PKY09_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB11607.1"
                     /translation="MTASEEERIRKAFSDKDWQEIKAQDTWQIFKIMSEFVEGFEKMS
                     RIGPCVSIFGSARTKPDTRYYLLAEEIAYQLTQSGYGVITGGGPGIMEAGNKGAQRAG
                     GKSVGLNIKLPFEQTSNPFVDHDKNLMFDYFFVRKVMFTKYAQGFVVLPGGFGTLDEF
                     FEALTLIQTKKIGRFPIVLVGKEYWSKLFEWLQTVMVDEGNILPEDLSLFKLVDEAHE
                     AKSHIDAFYSKYLLKPNF"
     gene            complement(24229..24993)
                     /locus_tag="PKY09_01765"
     CDS             complement(24229..24993)
                     /locus_tag="PKY09_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB11608.1"
                     /translation="MLTSSEIKQIKSLKDKANRHEQQKFVVEGEKMVAELAHSAFTII
                     AVYATDEWIEQSSNDIKGLLKEKLIKVKTKELERISSLKQPQSVLALVAMPQQPETEI
                     QFKDIILAFDDISDPGNLGSIIRIADWFGIKQIVCSSTSVDLYNPKTIQATMGSIFRV
                     NVFYTDLEKWVSANKQNATFYATLLNGKSVYAVAKKRPAVLVFGNESRGVSQSIQQLI
                     DQAISIPKFPDEKSSPVESLNIAMSAAIFCSEWRRD"
     gene            25065..27356
                     /locus_tag="PKY09_01770"
     CDS             25065..27356
                     /locus_tag="PKY09_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB11609.1"
                     /translation="MRKRQYGILFTLILLVASCSPFKQLDKGEYLLNRNQVIIDKPEL
                     REGVKAIIKQKANRRILGLFRFHLAVYSMGNRGKPTKFKNWLKNTVGEEPEVLDTLFT
                     KKSIQQIKQFMSKNGYFDAEVKDSTIYRKKKAIVRYTIIAHKPSFIKYVARECADDNL
                     DSLVLKDTSTSKLQAGIIYNEGILQGDRERLTKKLRNEGYFQFSPQFVSYRVYTSDTT
                     KNIAVFQDVAAFSNPDSTVSSVAMPHTKFKLNRIIFSPDYDPLSLNASMIQYDTVAYK
                     GYLFVKRAGKKMPVKPEVIVNHTFLKPDAVFRQSNADYTYKGLNLLGMYRFVNINYNA
                     VDSNKLDCFINLGATPKQEYKTEFEGTNNGGNLGVAGDFTYRNKNVFRGAEMFEFKIR
                     GALESQKKFTDDKDNKVLLLFNTYELGVENSIYLPKAIKPFNRLFKNIAANPVTVFST
                     NYNIQNRPEFNRSILDFSASLEWRKGRFIKQIVTPFQINFVNVKLEPEFKARLESLND
                     PVLFSSYDNHLISNGRYTLIYNNQQLNEPGNFKFFRTNIEIAGNTLYWFRQIEGADKD
                     VNNSYTILNKRFAQYFRPDADFRFYQNINAHNSIVYRIAAGIGIAYGNSKIIPFEKSF
                     YAGGSNDLRAFRARNVGPGNFTSVNNFERTGDIKINGNIEYRFDILKILKGAFFLDAG
                     NIWVRKKETSRPDAEFELDRFYKQLAVGSGLGFRFDFTFFIFRLDIGVPIVDPQYKSD
                     DKVIVDEVKPRSLIYNFGIGYPF"
     gene            complement(27363..27749)
                     /locus_tag="PKY09_01775"
     CDS             complement(27363..27749)
                     /locus_tag="PKY09_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNB11610.1"
                     /translation="MNIQLEKVRIDKWMWAVRIFKTRSQAAEACARGKIFIDGVSVKA
                     SRLIKTNEILSIRRGSFTLVFQVIQPIENRVSAAIKDQFCKDLTSADEYEKIKMHSLA
                     VRTYRQHNEGRPTKKERRALDDFLDW"
     gene            complement(27776..29902)
                     /locus_tag="PKY09_01780"
     CDS             complement(27776..29902)
                     /locus_tag="PKY09_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNB11611.1"
                     /translation="MTDSLLLTRFRSNTGISVHQLEQTIKLFEEGATVPFIARYRKEK
                     TGGLSDENLLELKKLLKEVEDFNNRKQFIINTLKAKGVFDLLKDKIAAATTLNELEDI
                     YLPFKEKRKNRAQNARDAGLEPLAQTLIRDDRNAKQLAEKYISSTFADTEKVLQGARD
                     IIAELISENHQVRKTFRQCFEQKSFIISKVIKSKLEEAVKYKDYFDYKEKITSCAAHR
                     LLAILRGDKEKLLRVSVMPDSTFLLNLLFDTIKSEGFYLNAQVKTAAEDACNRLLIPS
                     MENETIGYYRLKAETESARLFAVVLKQLLLAPPLGQKNILAIDPGFKSGCKIVCLDKN
                     GRLLHNETIYPHPPQSQQKIASNKISTLVSQYKIDAIAIGDGTAGRETGTFIQRLRFD
                     RKIQVFMVNEDGASVYSATAEARKEFPEYDVTVRGAVSIGRRLMDPLAELIKINPRSL
                     GIGQYQHEIDEMMLNSALDQTIEHCVNTVGVNLETASEKLLTYVSGIGEVMAKKIIDA
                     RTHSGIHSRSELKKIKGMGEKTFEMCAGFIRINGDNPLDKSAVHPENYTLVERMAEDI
                     DTTITGLIGNESLIQKIDITKYVTDEVGLPTLKDIIAELSKPGRDVRGIIKVFEFNAE
                     IKSINDLQPDMLLPGIVTNITNFGAFVDIGIKKDGLVHISEISDAFISDISTAVRLNQ
                     QVMVKVLSADITTGRIQFSMKGIQQP"
     gene            complement(29934..30323)
                     /locus_tag="PKY09_01785"
     CDS             complement(29934..30323)
                     /locus_tag="PKY09_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11612.1"
                     /translation="MKRTFTFLSVATAFCVIAATVIMPSCKSSKSTTASSSTPITVPD
                     KPSYAVHVKPIVDAACGTKCHSPAKKADGLDLSYYEGVKDACEKHKLLGSIKHESGVK
                     AMPRMAPQLSKEAIATIEKWINDGYAQ"
     gene            30420..31013
                     /locus_tag="PKY09_01790"
     CDS             30420..31013
                     /locus_tag="PKY09_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNB11613.1"
                     /translation="MSYKPNDYYARKAKQENFAARSIYKLEEIDLKHKLFKQGDNLLD
                     LGASPGSWLQYASKKAGEKGKVFGIDLKPVEVKLPNVFTVQDNIETFDLQTLFDRYGF
                     NDKFDVVMSDMAPNTTGSRFTDQARSFDLSMLALQTAKRFLRPGGHFVCKIFDGEDAM
                     AFRDEVKSCFGECFVLRPKSTRNSSKEIFVTGKYFKA"
     gene            31104..32861
                     /locus_tag="PKY09_01795"
     CDS             31104..32861
                     /locus_tag="PKY09_01795"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11614.1"
                     /translation="MKRILLCLAFTVVGFLSFAQCSIDSTQTDPGIYPDTLLPATVNQ
                     PYTQDITFVMITDTMGITITNFNIANITGLPVGMTWQCNNFVNGCNYNPSQSIYGCVN
                     LSGTPLIAGVYNATVTVIATLQVLGDQTISYTLPFTVLPDTVSNPGFSMTNSSGCLPL
                     SVTFVNNNPGQASYLWDFGNGIQSNLENPPVQIYNVPGDYVVTQSVTANGSPQYFLTD
                     ITVASIPDNYGAPVDVPDMYFYLYDSQGNQIYDSHPSVSNTNAPLSWTLPNIPLQNSN
                     YTLHVWDEDGGLFGADDDLGSVTFPGWSASGTATGTLSGVSGSLVVNYSIMQVPVATT
                     IHTDTVHVFAVPDTPTVSANIPLILCAGDTVVLTCNDTLAIQWYESGNLLIGETNSDL
                     FLTTSGNFFAVVTNAHGCTAVSAAINVQVNPNPPKPTFFVNGNTLNCVLTGYMLQWYY
                     NGAAMPGETSLQLTATQQGTYYVVATDSVTGCTSVSDPLVFTPVGLDQPQSVIAAVLF
                     PNPADKSVVVQLTSVRNIFTTIQLLDISGRVLQQNNFTLQPGTTQLPFDLSDISKGVY
                     LIKITQENASKTLRLMINR"
     gene            complement(32865..35021)
                     /locus_tag="PKY09_01800"
     CDS             complement(32865..35021)
                     /locus_tag="PKY09_01800"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNB11615.1"
                     /translation="MFKRTFLTAVLVLSQLFSKADEGMWLPTLLKTLNADAMYSKGLK
                     IPIDSLFSLNSSSIKDAIVQFGGGCTGEIIGKSGLLLTNYHCGYSQVVQHSSVKDNYL
                     TNGFWAMNLNEELPCTGLTATFIVRMEDVTESILPFINDSLKETEKTVKIKELSQIIV
                     QKAIQGTHYDAEVKPFYYGNKYFLIVTEKFKDVRLVGAPPSSIGKFADETDNWMWPRQ
                     TGDFALFRIYADKSNNPADYSKENIPYKPKYVLPISLNGINENDFTMVYGFPGRTQEY
                     LPMEAVRTLMNHTDPVRIELRKIRLEQMEQAMEHNDTVRIQYSSKHSSIKNGYTKWQG
                     EILGLKRLNALAVKSQHEFDFFQWAKKNAKTEYQKVVSELNQLHIKQRPLSIAADYYF
                     EAIKGVELLTFAETFSRLEKICSTNPVDEKALSELKTNLLKSTAGYFKNYDVAIDKAI
                     MPMLLKEYIDKTPSGLEADLSFIAKKHKNNISSYCDYVLKKSVFADQKRAENFIQSFT
                     PNKLSKLLNDPAYSLIKQCSLAYALKVLPEMTKITLEINNLQKVYMKGLMEIAAENKK
                     NIYPDANFTLRVTYGKVEGYEPRDGIAYKYYTTLDGVAEKYDSTNYDFNVPQRLLELH
                     KKKDYGNYATNGKIIPCFLASNHTTGGNSGSPVLNGKGQLIGINFDRVWEGTMSDIMF
                     DPEKCRNITLDIRYVLFIVDKFAGAKWLIEEMNLVQ"
     gene            35151..35882
                     /locus_tag="PKY09_01805"
     CDS             35151..35882
                     /locus_tag="PKY09_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11616.1"
                     /translation="MKKIAQIAIISSVLSTAGYAQSAESDSHLILSIVSLPASQITFK
                     FEEGKIFYVKIPSEKLRLKVKVPDEPTFKMLTHEQQRKQQEEEDKKFQARQDAVVQNT
                     QAIAGNYNLTDITLEQLQAQEKEVVVANDDLTVKVVPLVGLPTDTILQQQQIEETVKE
                     NKTVQQELVGTSVITSQKTIQKPAAALINISYAETSDQATAKVLDTGNGSKIDNLNLK
                     SESIPVSIRRQHNSVIGRYIPVVIN"
     gene            35994..36263
                     /locus_tag="PKY09_01810"
     CDS             35994..36263
                     /locus_tag="PKY09_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNB11617.1"
                     /translation="MVKQAEQVKQRKKTVSNDYHIVLYNDDVNTFDFVIESLMEICNH
                     EREQAEQCSIIVHNNGRCSVKDGSVSVLQPMCKALVQRGLTAEVE"
     gene            36340..37137
                     /locus_tag="PKY09_01815"
     CDS             36340..37137
                     /locus_tag="PKY09_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNB11618.1"
                     /translation="MKGTIKILLLIFFVSCSNVPLTERKQMNLLPESQMVAMALTSYN
                     DFLKEHPPMVNSNSDAQMVKRVGERVKDAVVQYMRDNNLYDRIKDYKWEFNLVDSKDV
                     NAWCMPGGKVVVYSGLLPVTKNEESLALVIGHEIAHAIARHGNERMSQQLLAATGGLA
                     LDVALINKPAETRQLFNTAYGVGANLGVLLPYSRLHETEADKLGLIFMAMAGYNPAIA
                     PDFWKRMAAVSGNKVPEILSTHPSNETRIHDLNKFMPTAMKYYKPRP"
     gene            37318..38025
                     /locus_tag="PKY09_01820"
     CDS             37318..38025
                     /locus_tag="PKY09_01820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB11619.1"
                     /translation="MLQYESSLFESEYIKLRNAEGRLYSDEMVAKLPYVTKEHIHYKE
                     WKIRRDSLRRLILFLQKNEKIKRILEIGCGNGWLCNQLHKSGYEVAGYDINQIELSQA
                     KRLFPHIEFFSQELLNSHSVKKYDVVLFAASLQYFKNALEVILFSMKQFLNDKGCVII
                     ADTFLYSENEDQKAKERSLEYFKKNNSEMGQFYFHHSTTILNLFDVAELKPQQSLLDL
                     ITGFKNPFKIYIIKAKS"
     gene            38030..38962
                     /locus_tag="PKY09_01825"
     CDS             38030..38962
                     /locus_tag="PKY09_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNB11620.1"
                     /translation="MSQICTKNTFEIRLSILKVLAYFKIFEHPLKLREIAALCNISVQ
                     ETEQNLSFLIEDEMIFGFEQYYSTSRNVAQYSGLRKVKEAEAEKYFIKLKKYAKLLAT
                     FPFVRGIAVSGSLSKGVMNPDGDIDYFIITKPGRLWICRTLMILFKKLFLFNSKKYFC
                     VNYFVDENNLQIRDKNIFTATEFVHLIPVFEHSCILNVFLFQNNWVHKYYPNLPDWNR
                     NYLVQCNIKLKNAFELLLKTSVFNPLEKLCHHITLYYWRKKFPNFKKEKFHLTMRSTK
                     GISKHHPSDFQTKVLRAFQIEMNEINSYLTVSEL"
     gene            38959..40389
                     /locus_tag="PKY09_01830"
     CDS             38959..40389
                     /locus_tag="PKY09_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNB11621.1"
                     /translation="MKVLITHSYYLQFDKKQLALSKPYPPLATLIVANVLRNLGNDVY
                     LHDVSFDSSLQPLKKRQKEVNPDLLLICDDGFNYLTKMCLTNMREAAFNLLRFAKSIG
                     VKTIISSSDSTDHYTDYLMSGADVVLLGEPESTLIELFDKGIRDFESIKGIAFKSNGN
                     VKCNERRKVITQLDKLPFPAWDLIDIKPYKRAWEKHNYFSINVSTTRGCPYKCNWCAK
                     PIYGNRYNVRSPEHVLNELKEMSRFFEFDHIWFCDDIFGLKPGWLDSFSKLLIESGMK
                     LKYKIQCRADLLQNEELIDNLSKSGADEIWIGAESGSQKILDAMDKGINVEQIYQAVR
                     LLKKYQMKPCLFLQFGYPGETKADILKTLKMVDELDPFDIGVSVSYPLPGTVFYEKVK
                     ASMKEKKNWKDSDELLLMYSGEYSATFYKALHRYVHKSFRAKQSWQELKKIFTLKNGN
                     IHKALLTAYYKPASIILRSGVNLKEK"
     gene            40398..41195
                     /locus_tag="PKY09_01835"
     CDS             40398..41195
                     /locus_tag="PKY09_01835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB11622.1"
                     /translation="METSVQAFSLQAKVFDDYNESNCLSRYCRDEIYKIALKYIPGNS
                     DILELNCGTGIDAIYFSKNHKVLATDGAEGMINVLNHKINKQNIPQIETKCISFDQLD
                     LIGNRKFDFIFSNFGGLNCTENLADVVNKLPLFLKDNGRIMLVMMPPICLWEHLHVFS
                     FNFKLAFRRWKKSGANAKVEGVKFKTYYYHPGFVSKLLKEKFKIVYLQGLCNIMPPEY
                     MHHYLKSSPMLFKMLSKADRFINRIFPFKQTGDYFVIVMEKKKICIV"
     gene            41183..42574
                     /locus_tag="PKY09_01840"
     CDS             41183..42574
                     /locus_tag="PKY09_01840"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HNB11623.1"
                     /translation="MYSVSKQFYIILILIASIIFFITGFLRFDGLYGQDAYSYLLQCK
                     NLFSENYRLNFYPPVYAFSGWLLHFIFHDFAISLQLVSLISVIVTTILIRKIILIQSR
                     NEQHAFVFSIVFFLLSPFILRSSVIVMSDALAMMFCTVCLYFQLKQKESSVFFVCLFF
                     LLAILTRYAAAPLLLPVLFYSLLRALRRKQFVYLLGGIGIIIVGFGLNALFWSNASGS
                     VNHQWLHLWKFVNIFRSSFNTPDGIQDYLLPNILYVFKLFVYPGFIPLFLFLLFFIQK
                     NELFNRKMILPTSSLLLYFIFLCGIPFQNDRFLLIAFPFASIIMFPSFSRLMERFKPP
                     LWIVVVVCIVHVVLACRAILPMYHRNIIETEIANALKPYQGKVLYSFDMDVALKGRGL
                     NFDFKNLLDTRYQSFEESSLMLFNEKKMGKQWAGRNPMINFNEAKSSYLLTVESELND
                     GWILYLVQKSKTR"
     gene            42571..43620
                     /locus_tag="PKY09_01845"
     CDS             42571..43620
                     /locus_tag="PKY09_01845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB11624.1"
                     /translation="MKVLWITPGFASDESDSTCIPPLQDLARALQEQNIELTIIALDY
                     PFNRKEYHWHGIRVYALGGANKKGLSRLFLLQRTWRLLKSIEENHEVDLVHSFWLTWP
                     AVVGQRFCKKYKLPHLQTIMGQDAMKNKFYSWFDFKYSAIVNLSQFANDKFYLSAGRQ
                     SDAIIPFGLGPISAAVVERDIDLINVGSVTGLKNQLLFLRIVNQIQQLKFNVKAVLIG
                     KFYDLHLIRKLKQFIAENHLEGIVELLEEMPRTEMFKYLGRAKVMIHTSTFESQGMVM
                     LEAIAHGCKVYSSGKGMHFNHELFSLLTDDLNENVNSIFSYLLKKKNPHGEKIVIIEQ
                     TANDYLDLYKKLLKK"
     gene            complement(43626..44381)
                     /locus_tag="PKY09_01850"
     CDS             complement(43626..44381)
                     /locus_tag="PKY09_01850"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNB11625.1"
                     /translation="MALQEEMEKQGLWLFKYRSMLPLVLLAGGLSYYLWSKAHHLRPF
                     PENSRSEEWFLYACMAVSFLGLLMRIYIVGHTPVNTSGRNTSEGQVADTINQTGMYSM
                     VRHPLYVGNFIMWLGIALFTASIWFVLLFIAVYWLYYERIMYAEEQFMRKKFGNAYTD
                     WAEKTPAFILEFSNYKKPSLPFSWKKVLKKEKNGFAAVFIVFLLFYAAGQLITNNAVQ
                     NNFLIYGTVFSIVFYVIIKIIRKQTTLLHESDR"
     gene            complement(44459..45397)
                     /locus_tag="PKY09_01855"
     CDS             complement(44459..45397)
                     /locus_tag="PKY09_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11626.1"
                     /translation="MKKQLILITSALALITLFYSCKKEDQQALDAKTKQFNDDSNNYK
                     AESDQADNDINNTINEIPAFGRGAGVVSSPLCGVTIDSSQLSQKILFFNFDGITPCFS
                     PSRTRSGQIKVQLTTGTNWHLAGSVLTITYNNFKITRLSDNKTITFNGVKTLTNVNGH
                     NWLTFLAGTSSFKYQSRAFNVNVAYDDGSTAVWNQANITEWSYVPSGNHGAYLIFTAN
                     GDSTVNNYSNSSMWGTNRYGQAFTTNYTTPIISNTYCGLWRPNSGELIHHVNASDFAL
                     TLGVDQNGNATTLDCAYGFKVSWTSNGNSNSVVLSY"
     gene            complement(45490..46332)
                     /locus_tag="PKY09_01860"
     CDS             complement(45490..46332)
                     /locus_tag="PKY09_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HNB11627.1"
                     /translation="MLLVADSGSTKTNWLLTDHKIFKTEIETIGFNPFFHSTEFILNE
                     LKKNPSLVKYANEIKDIKFFGSGCSSDDRKNIVASAFKSMFPNATVLVDHDMLGAALA
                     ACFDTAGLVAILGTGSNIAFWDGKHLHPTNHGLGYILGDEGSGSYFGRKLMTHFLYGI
                     MPDDLKKKFYEHYHLTKESAIKNVYHLPGANVYMASFAKFLSYEASHPYIQHLIYKGL
                     TEFADTSILAYPQHQNYPVHFIGSIAFHFRSILERIASEKKFFVGKTIIRPVDDLLVY
                     FKQK"
     gene            complement(46355..47329)
                     /gene="pfkA"
                     /locus_tag="PKY09_01865"
     CDS             complement(46355..47329)
                     /gene="pfkA"
                     /locus_tag="PKY09_01865"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNB11628.1"
                     /translation="MNIQKIAILTSGGDSPGMNACIRSVVRCAISQNRNVIGIMRGYE
                     GMIHGEFVELNARSVSNILQRGGTILKTARSKAFMTAEGMKKAYDNLCEEGIEGIIAI
                     GGDGTFKGAVAFNKFNNIPFIGIPGTIDNDLYGTDYTIGFDTALNTIIDAVDKIKDTA
                     ASHDRLFFVEVMGRDSGCLALYAGIACGAEEILMPEQKTDINALVEKLKESVLRRKTS
                     SVVIVAEGDDGGGAFKIAEAVKKSFDYDIKVTVLGHIQRGGSPTAKDRLMATRFGAEA
                     VAALLNGQTNKMTGWNGKEVTLIDLEKVVKQHNPFDKRLVSYNNFLSL"
     gene            47422..49221
                     /locus_tag="PKY09_01870"
     CDS             47422..49221
                     /locus_tag="PKY09_01870"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNB11629.1"
                     /translation="MTKFTLLAAFAVLLSGASIAQDRVPVNLNPAFGARSQSVTKTEY
                     TNPGQLIPRNGNSAINVAFSDDFSQQNDTTSLIARGYLPYYRGTGPQGAQATWFQPDG
                     TVFPAYNGAATEYVAANYQVVTGTNNIDSWLVTPALNVSAGDIIAFYARSVAGSTWVD
                     SIRVMYSAAGDSTPEGLNWVELGRFQASITGTWDYINYPVTAAGTTARFAIRYTVADG
                     GPNGSNSNFIGVDQLDIYTPQAIDASVFAISNMNSGCGLSATTPVDVSIFNWGGSAIS
                     GFPVSFVVDNGTPVTETFTGTIPAGGSSSFLFTGTADFSAVGPHTIKAYSSLPSDGNA
                     SNDTTIATVTNIPPVVLGSTPVTEGFEMASGTTPPPGWTIEDTDGDGNIWDFATTYTH
                     NGLVCARLGVGNPLAATPENWLFSPCLDLTAGTDYRIEYWYKSFTATATSYQLELRYG
                     SAATGAAMTNNVAVDPLFTDSTYHLASHTFSPTSSGVYNIGINGFGSGVDNSIRIDDA
                     SMVVVTGIKDNDLSSNVTLMPNPTTTELLVSAKLYGNSTLTVYNQLGQEVIKQNYNEP
                     FRITLDIQSLNKGVYTLKINNADGVVVNKFVKQ"
     gene            49284..49643
                     /locus_tag="PKY09_01875"
     CDS             49284..49643
                     /locus_tag="PKY09_01875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNB11630.1"
                     /translation="MKTSDFFNTDEISFFEKVYQVVRLIPKGRVTSYGAIAKYLGSAQ
                     SSRMVGWAMNNSSRIKPKVPAHRVVNRNGMLTGKHFFGANDAMQYLLEKEGIKVSDDK
                     IIDFKKHFWDPSVELIL"
     gene            49674..50774
                     /locus_tag="PKY09_01880"
     CDS             49674..50774
                     /locus_tag="PKY09_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNB11631.1"
                     /translation="MQITKEKVLEALSYVQEPDLKKDLVTLNMIKDIEIEGQHVSFTV
                     ILTTPACPLKAEIERACKNAIVHFVDKDAVVTVNMTANVTTQRSMDNEMMKGVKNIIA
                     VASGKGGVGKSTVAANLAVSLAKNGAKVALVDADIFGPSVPIMFGSEHEHPFIEEVNG
                     RQLMIPVEKHGVKLISIGYLADPSQAIVWRGPMASKALKQLFSDVNWGDIDYMIVDLP
                     PGTSDIHLTLISAVPLTGAVVVTTPQDVALADARKAIAMFQLPSVNIPVLGLIENMSW
                     FTPEELPDNKYYLFGKDGGQKLAAEMNVKLLGQVPLVQSVREGGDMGNPIATGSSIVA
                     AAFNDLSQSVAQRVAIRNAVIEPAQRVPSAGY"
     gene            50783..51046
                     /locus_tag="PKY09_01885"
     CDS             50783..51046
                     /locus_tag="PKY09_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNB11632.1"
                     /translation="MMSESVKNNLKLRVEQALDTIRPYLKSDNGDVTVLDITPQNVVK
                     LRFEGACSSCTMSAMTFKAGVEETILRMVPEITAVEALNVENV"
     gene            51116..51832
                     /locus_tag="PKY09_01890"
     CDS             51116..51832
                     /locus_tag="PKY09_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11633.1"
                     /translation="MKIIAVIFYLLFFTGIIHAQTFVVFKGDTINRKDTKGLKQGVWK
                     KFYRTDSLFSVTTFKDNKQTGTTKFFYESGKIKSTLIWKKPGQFAFAESFFEEGKKMS
                     DGYYLNEQKDSTWHYYNEEGKITSQESYRKGIKQGKWLTFYPEGGKVEEKNYVNGKLE
                     GPYLKFFKDGKLMLKATYKNDKLTGGYTIYNADGGYYTTGQYSAGDRAGKWTVFNEKG
                     KPWIESQYKNGFEVKTDTLQ"
     gene            51854..52960
                     /gene="mnmA"
                     /locus_tag="PKY09_01895"
     CDS             51854..52960
                     /gene="mnmA"
                     /locus_tag="PKY09_01895"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNB11634.1"
                     /translation="MSKKGRILVAMSGGIDSSVTSMMLHDEGYEVIGITMKTWDYASS
                     GTSGKETGCCSLDSINDARSIAVEKGFPHYILDIRGEFGDFVIDNFVDEYLAGRTPNP
                     CVLCNTHIKWEALIKRADMLDCEFIATGHYASVRSEGGRYVVSRGKDLSKDQSYVLWG
                     LSQKNLARTRFPLGNYKKSEIRSMAFEMGFKELANKSESYEICFVPDNDYRAFLKTRV
                     EGIEEKLDGGVFVNTKNEVIGKHKGYPFYTIGQRKGLEVAFGKPMYVTEIKPNTNTVV
                     LGELDDLQKTAMKVRGLNLVKYESLSKPTEVLTKIRYKDPGTMSVITQTSANSADVEF
                     HAPVTAVAPGQSAVFYEGNDVVGGGFIDKEYDKV"
     gene            52947..53618
                     /locus_tag="PKY09_01900"
     CDS             52947..53618
                     /locus_tag="PKY09_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11635.1"
                     /translation="MTKSKNIFILLNLVLVLMMGCTKDKETVSAIDYRYYPIAVGDSM
                     IYQVSLFNKDLNTYDSTYQLLERVESVFNDNEGRPTFRLERYVRKSDADAWSIYKVWT
                     SNKTSTSIEKQEDNITYIKLIFPVKLNKTWNGNAKNIFADDFEDYKIISLNEPLQSGN
                     YSFDSTLNVRQIDYDYGFEKRKHFEIYASGVGMIYKDEYDSLQGSPLGKIYHYTETLL
                     YHNKK"
     gene            53621..55291
                     /locus_tag="PKY09_01905"
     CDS             53621..55291
                     /locus_tag="PKY09_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB11636.1"
                     /translation="MKKIYLLILIFITSQELYSQNRYAVMLTDKNNSTYSFSNPSAYL
                     SQRAINRRLQFGITLDSLDLPVNSTYLAAIQNTGAVILNTSRWLNEVTVDVSANPGAL
                     SAINALPFVKQTKLAARTTNRSNSKYSFEMESLMQRQSQTQKVASTSSFYNYGNALNQ
                     IQMLHGDNLHDLGFRGDGKIIAMLDAGFLRADSMTAFDSLRAHNRILSTYDFVDHNSN
                     VYDDHTHGSMCFSIIGANDPGNIVGTAPEASFLLYRSEDAATEKLIEEYNWATAAETA
                     DSAGADIISSSLGYSVFEDSTMNHTYNDMDGRTAPISIAANIASRKGIVVVSSAGNEG
                     NGQWHYITAPADADSILTIGAVDAAGVYASFSGTGPSFDGRIKPTVVAQGQGTFVSDP
                     YSNVVFSGNGTSFSCPVIAGLVASLWQAIPSANNMQIINAIVQSASQYSNPDSLLGYG
                     IPNFDSARNSLLAVFNPYYGKGDFIEKIFPNPFKNQLNINFYADSNSQYTSEIFDLSG
                     KRVMVKHGEFVPFTINNINLATANMGKGVYLLRITTDKNVFQARILKQ"
     gene            55298..56314
                     /locus_tag="PKY09_01910"
     CDS             55298..56314
                     /locus_tag="PKY09_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNB11637.1"
                     /translation="MSSIGKKLKILGTNFAFLILFLSIAEVGLRIANIGYDNAAFEPD
                     SVLHHIHRPDYSFIHSNAAEHEYGNIAVRYDKMGCVVNPHQKKTEHCREKIWFFGDSY
                     IEALQVKYDSSVTGLLEMENPDVCFKNFAVGAYSPILHYLKLKQLLKSEKPDIVFLFL
                     YANDPSEDKQYLKEAKIYHGEVAAVDGGMPQWKYEIIRNSNLLRLIRKFYMQMTVKLE
                     NKQRKEEYVLSENNVILAEPTLTYLEKFVMLCLNKNVKVVVSCIPSKTDVLRSDFNSA
                     SFSNQVKAWCSGKCDFLDLQEPLQKFYLATNTSPYFKNDIHFNNYGHRIVARTIVLAQ
                     IIKD"
     gene            complement(56291..56761)
                     /locus_tag="PKY09_01915"
     CDS             complement(56291..56761)
                     /locus_tag="PKY09_01915"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNB11638.1"
                     /translation="MSLNINHPAPLFESFNSEKQPFSLEQLKGKNVLLLFFPAAFTST
                     CTKELCSVRDNLSYYNNLNCTVIGISTDTIYSLAKYKEEQKLNFQLVSDFNKEIGKIY
                     HAQYELFGLGMKGITKRAAFLIDKNGILRYIEVLENAGEIPDFNKIKSVLDNLS"
     gene            complement(56767..>57898)
                     /locus_tag="PKY09_01920"
     CDS             complement(56767..>57898)
                     /locus_tag="PKY09_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11639.1"
                     /translation="LIILFLFSWFQSTAQHFVLYGERNLNDTILSIYFDRNGKPYPDF
                     FIADSALNISAGSLDTFYLNNPNEFAAICSLYKCRFSDYSISNISILNDSIFQTNADK
                     INNRTKEVMVPTFMIHGFRKSYVNKPGVFTSVIDYGLLMEAINRLSTEKHSYIRVFWD
                     GSYLDISEGVKQMKQIFTVFEKVQLNAISVGYGLRQILSRTHSPEVNIISHSLGAAVV
                     TNALFDTTVNNYPTPNNRNINICLIAPAISGIEAFKSYYSRKSTFPYKKSDNYNLFVL
                     YNKKDFVLRKKDYFLELFGPGPRKHGNTTLGCNANNEAIKLKDYFIQHFPNSKITLIN
                     MTDLIDKCHHLRCYCEGENLKTLVHLMNHTNVNSNRNKNLNN"
BASE COUNT        18194 a         9842 c        11157 g        18705 t
ORIGIN      
        1 aattaattta tatttgtaaa tgcttttgaa ttattttttt cacagactgc cgttagcggt
       61 cattgtagga cgactaccca actttaagta agacaacaga atgaaaagaa gaattacata
      121 catcgtgctg acaattttgg ctttgacagc gtgtaacgaa aacaagaaag aagcaacaaa
      181 taacagtatg acaacatcgg taacagagaa agcaacaatg aaaagtggtt tttccgaagt
      241 aaacggaata aaaatgtatt acgaaattta tggacaaggc aaaccccttt ttcttttaca
      301 tggtggcggt tcaaccattc agacaacttt cggcagagta attcctctat tctcaaagga
      361 cagacaacta atttgtgttg aattacaagc acatggacgg actggtgacc gtaatactcc
      421 catttcattt gaacaggatg cagacgatgt tgctgcttta cttaaaaatt taaatgttca
      481 caaagctgac atttttggtt ttagcaatgg aggcaacaca gctttacaag ttgcaattag
      541 acatcctgaa gtttgtaata aagtagttgc aggttctgtc ttacttaaac gtaacgggac
      601 attcccacag ttttgggagt ttatgaaaaa tggaactttt gaccaaatgc ctcagcaata
      661 caaagacgca tttatcaaag taaatcccga cagcacaaaa ctgataacta tgtatcaaaa
      721 atgtgcagac cgagtaataa acctaaaaga ctttacagac gaacaaataa aatcaattaa
      781 agctcctgtt ttactcataa atggtgacca agatgttgca accgctgaac acattgtggc
      841 tatgtcaaag ttaattccga actgcaaatt agcaattatt ccaggcggac atggagcata
      901 tattggagag attacgacct taaacgctga ctataaagag agtgaattta tcattccatt
      961 aattgaaaaa tttcttgacg aaaaaaccaa acaataatgc tgagtgataa caacaaagaa
     1021 gaagaacgac ccggtaacat gtgtttggcg tcatgcgggg tgaagtgctt aaattcaagt
     1081 tcggttttcc aattggactt tagtgctgtg ttgacagttt tgagctccga aatcccgcac
     1141 gaacgccaag cgcaaaaacg tcaggctgtg aaaaaagtca gtttctctta aaatattatt
     1201 gctaaaatta tcgcacaagc attcgcttgc gctttaagac tgtttgattt taagcagcaa
     1261 gattatgttg taaaaaattg agtagcctaa aatggctgat atgaagtctt attttagaaa
     1321 aacaagtatc tccgggatac ttaggtgtcc aattttttag tacttccatc agtttatcaa
     1381 agcctaagat atttttggct cgttttatat tgtaaactgt catgatgagt gccaactctc
     1441 cgtttacttt ctctaatcct cgcaggttgg tgtaatacaa attccactgc cgctttattg
     1501 taccaaagat atgttcgtta atctcttgtc gctttcggta tagttcaggg ttgcattcgt
     1561 agttgtgttt gtttcgttct actgcctcgg aatactcact gcgttctatc tctcttctcc
     1621 catcgtgtct gcctgtacac aggtgtttta caggacaagt gttacaagcg gctgttcggt
     1681 attttttaaa ttgataagag atatcttcac tacgtttctt agtgtgccaa tttccagtac
     1741 tacttagtgt ttgaccttat gtgcatgaat aggtgttccc agcttttttc ttctcacttt
     1801 gcaccaacgt ctgaataccg agctctttta aatctaattt atctaaaaaa gcatctataa
     1861 aacgaatttc gttgtccttg gttatctggc tctctaagtt tgaaaatgta atttgttcgc
     1921 ggttgacacc ttgtatttgt ggcattttta tttgctgttt tactccttaa aactatggaa
     1981 attaatttat atttgtaaat gattttagat tattttttca cgacttgtcc cgattgctcg
     2041 ggagactgcc gttatgttcc attcaacaat aaactcatca atcataaatt cagatattaa
     2101 attaatttcc atatctttga aacagagata ctaaataaat taaaatgtac aaaccaattg
     2161 aaataaatag agaaaatctg accattatgg gagttaagtt tcctaatatt aggtcattgg
     2221 agagtactgc aaatgcttta ggaactaaca tgttcgaagg gtttgaacca actaaagaac
     2281 tcattcaatt atatttagat tggaaaaatg gagagattat agaaacagat ttcttgagta
     2341 gattatactc tatttatgga aaatagctat agatacattg atcctgattt caaatacact
     2401 aataaaaatg gtgtattata caatttagct aacatagaaa atgaggacgt tctcgcagtt
     2461 tttgaaagct taaaagtttc taaaaggctt gaagaattat atgaaaaccc tatccaaatc
     2521 atagattccg agtcattaat ttacatacat cgttacttat ttcaagatgt ttacgaatgg
     2581 gcaggtaaaa cgagaactgt aaatataagt aaagacggaa aaccgttttt tgaaggtgaa
     2641 cgatttgaaa caggttttaa gtatgtaaac agtttaataa ctgattttaa aaacattgag
     2701 attaatgata agaggaaaat tgcgagaaca cttgccgaaa ttttggataa tgtcaatttc
     2761 ttacatcctt ttcgtgaagg aaatggaaga actcaacgtg aatttttgag attattggct
     2821 ttagaaaaag ggtttacatt aaatctcaac cctcctgaca ataaagaaat ctttgagaga
     2881 tatatgaacg gaaccattaa tagtgacgtt gaagttttat cccatttgat attcgaaata
     2941 atcaattaaa acatgaacga aagcataaca tgctattact cgcaagccga aataaccgct
     3001 tcgattagca tttttcgtta gaaaaaaatc ttatcttcgt atctaagttt aatgcttaaa
     3061 gtcggtcagc gagtagtagc tttccgttgg tagcaaactt atcgtgctgt gctattgccc
     3121 ttttgcatgc tgcattgata gaaatataaa gttccaaaca acgtagttgt gctttctttt
     3181 ttttgaatga taaactgatt ttgctttagt aagttagtga atataccttt ttttaaatca
     3241 tcagttgttt ttctgccacc aacggtttgt aaaataaaaa accctattgt agaaagtatt
     3301 gttttggggt ttccccaatc cataatgagc aaatgccctt cattagttaa tattcgattt
     3361 atttcatgca aagttttaag tttttcatcc ggaagtaaat tgcagaatag cagactggaa
     3421 attactgtat gagcattttt gttttcaaaa gggagaagtg atgaggaagc atgaatcaac
     3481 ataattttat tttgtgaaat tttattcagt ttcccctcta atatggataa tgctcttttg
     3541 tctgcatcta ctgcgtaaat agggacagta gtaaattttt tggcaagggg aagtgttaac
     3601 cctccggtac tacagcctaa ttcaatgatg ttttgagggt tcagcttctc aaccatgtca
     3661 atcattgtct gacgcaactt tttctctggc aacaatatgc caaaaagaaa atcataaaaa
     3721 ggagttagaa aatgaaagcg tagtgatgga acaaattcag tcacatcatc agttattagg
     3781 tgtacattcc tccatttaca tgcagcacct gaccggttat gtatgtactc aaatcagatg
     3841 caaaaaataa aactgcatta gcaatatcat ctacggttcc accgcgtttc atgggtataa
     3901 catctgtcca gccttttaca gttgcagggt ctaatgcgcc tgtcatttct gtttcaataa
     3961 aaccaggggc tatggcattg caacgaatat tacgggagcc taattccaat gctacagatt
     4021 tcgtaaagcc aatcattcct gctttgcttg ctgcataatt ggcttgtccg gcattacctt
     4081 tttcaccaac aacagacgta atgtttataa tagagcctga tcgttgcttg agcattgatt
     4141 tctgaatatg ctttgtagta ttaaaacaag atttcaggtt ggtacgaagc acttcatcaa
     4201 attgctcttc tgtcattcgc atcagcaaat tatcacgtgt tatgcctgca ttgtttacca
     4261 gaatatctat tttttcaaaa tctgccagaa cctgattaat cagttgctcg gaagcattaa
     4321 actgcgatgc atcactttga taacctttag ctttcacacc atgtgatttt aaagtgtttt
     4381 ctaattccat tcctttttca ggtgatgata gataggtaaa ggcaatgtgt gcaccatgtt
     4441 gtgccaaggt aagggcaata cctcttccaa taccgcgcga ggcaccggta attaatacaa
     4501 ctttgttttc gagtagtttc ataccattag attttgacaa ttgaattaaa gttctacatt
     4561 tgcggttcaa atatatagct aaactttaaa agtgatgaaa aaactattgt tcagtgttgc
     4621 tttagtagca atggcgttga ctgtaaacgc acaaaaattt aaaattggtt taggtggagg
     4681 ctttaacagc acctggctga tgaataaaaa cgtgtttgat gccgatgatg atttgaatgt
     4741 tgccatgtcc tttggcggtc aattcggatt gaaaacagaa tactatttta atgataaatt
     4801 agctctcgaa ctgggaatta tgcgtgccgg tcacaatcaa aagtatgatt taaatgatgg
     4861 taatgaccag ctaacattaa aattacgcta tttagatgtt ccattattac tgcgtttagg
     4921 tggtgataag ggtggatatt ttgaatttgg acctcaattc agttttctga atagtgccaa
     4981 agatgttgat gacgatggtg attcacgtaa tgtgaaagga tcacttaaag gagccaatat
     5041 tggtcttgta attggttttg gagttgatgt tgatttaagc gagcaattta cattgactgc
     5101 aggaatgcgt ttgggttatg gtttcagcga tgttactaag aagtattcca atgcaattga
     5161 tatggcacaa gatatggatg atatgtcagg agctacttac tgggctaact ttaaagatgg
     5221 cacatcagga gatttcagct ataagactac ctcaagagta tatggtggta ttcatttagg
     5281 tgttctttat actattccaa caggaaaata atagaataat caaatataaa attaaccgct
     5341 tcattattgg agcggttttt ttatggatta atggtttatc tatttttgac atgaatttta
     5401 attttaagta ataagtataa aatgtgtggc atagccggat ttgttgatga tagtggtgat
     5461 ggtgagaaaa cgctttcggc aatgcttaaa gctattgcac accgtgggcc tgatgcaagc
     5521 aaaatattca tcaatcagaa tgtacatctc ggacataaca gactcagcat aattgacctg
     5581 agttccgatg ccgatcagcc catgcacgac agtggactga ctattgttta caatggagaa
     5641 atttacaatt acattgaact tcggacagaa ttggaaagga atggcgagat ctttaaaaca
     5701 aaaagcgata cagaggttat cttaaaagcc tatcagcagt ggggagaaaa gtgtgttgaa
     5761 cattttgttg gcatgtgggc atttgcctta tgggataaaa atgaaaacaa acttttttgt
     5821 tcgcgcgaca gattcgggat aaaaccgttt tactacataa acaacaataa caaattttat
     5881 ttcggatcag agtataaggc attaaaacct actcctgttt tttcaaacga aattaatcag
     5941 tatcaggtgt tgcgtggttt acaaatggga tggatttgct atgaggacga aacctacttt
     6001 gatgtcatca aagcattgcc tgcagcgcat agtttggtgt attatcgtga taaagcagaa
     6061 attaaaatct atcgttactg gtcgttgcag acaggaattt attccaactt aagttttgat
     6121 gaaaagtgcg caacatttta tgagttattc tctcagtcag tcaagctgca ttatagaagt
     6181 gatgtgcctg tggcaagttg ccttagcgga ggattagaca gcagcgccat tgtgagcatg
     6241 gttcaagaac agcatcccgg aatgacctat aagaccttta gcatttatta tgaaggtaaa
     6301 ggcgatgtgg atgagcgtcc atttgtgaat gaagtattaa aaaagtatcc ttcactgaaa
     6361 ccatattatt tttcaccttc cgatagtcag gtaacagaac attttcatca tgccttatat
     6421 cacgctgatg tgccaagtac aggttcatcc tttatttctc aatattttct gatgaaactg
     6481 attaaagagc agggaataaa agttgtgttg gatgggcaag gtgctgatga gtatctggca
     6541 gggtacaaac atacatttta tcgtatggtt gccgatgtgc tttcgtcagg acaggttagt
     6601 caggcattgg cacttacagg tattgtgtcc aaaaaccaag cgttgtcttc taagcagcag
     6661 tttgcacatt taggcaaaag tttattgagt gttgcaaaga gcgaacaaaa attatataca
     6721 cttgaatacc tgaagtatta tccatttatg ggtaaggata aacatattcc attttggtta
     6781 gacaaggttg aaggcaacag aaccgataac tttttgtatc atctcctttt tactacaagt
     6841 ctgccatcac tgctgcaata cgaagaccgt aacagtatgg cattttccat tgaatcgcgc
     6901 gtgccgtttt tagatcaccg actggttgaa ttttcttttg gattaagaaa tgaagagaaa
     6961 gctaatggta tttacaccaa atggataatg cgaagtgcat tacagcatgt attaccacct
     7021 aaaattgcct ggcgtaagga taagaaaggc tttgtgacac cgggcgaggt gaaatggcta
     7081 agaggaccat taacacactt aatagagagt gatttcagta aactcgattt tctgaatcag
     7141 aataaaataa agagaataat tgcaaattat aaacatggtg acaatagcca tgccttgctt
     7201 gtctggaggc tatgtacttt agcttactgg caaaaatatt tttgttaact tttacaggtc
     7261 gttggctttt tcaatcaatt cagcaatatc aagaactttt acagaatctt ctttgttgaa
     7321 atttttaaca ccatcggtaa gcatagtcat gcagaatggg cagccaacag ccacataatc
     7381 aggtttggct tctaatattt cttctgttcg ttcaatgtta atgtctttag ttccattttc
     7441 gggttcttta aacatctgtg caccacctgc accacagcaa aatccgttgg cacggcttct
     7501 tttcatctca aataattcgg catccaattt tttaatcaca tcacgaggtg cttcatacac
     7561 atcgttgcct cgtcctaaat aacatgggtc atgaaaaaca atcttctttc ctaaaaattt
     7621 tccaccatta atgataagct ttccatcatt aatcaattgc tgaagcaaca tgctatgatg
     7681 tacaacttca taatgacctc ctaactgagg atattcattt ttaagtgtat taaaacaatg
     7741 tgggcaggca gtgacaattt ttgtaacatt atagccattc atgatttgaa tgttcatcaa
     7801 tgcctgcatc tgaaataaaa attcattgcc tgcacgtttt gccggatcgc cattacaact
     7861 ttcttctgtg ccaagaacag caaatgaaat attacaggag tttaaaatct tagcaacagc
     7921 ttttgttatt ttttgtgcac gctgatcaaa acttcctgca catccaaccc aaaataaaat
     7981 gtcgggagat tcattagctg caagaaaatc agccattgtt cttactttta aagtcatgat
     8041 gtattatttt ctaatcaatc ttcatttttc caattcaggc ggtcagcagg tgaaaactgc
     8101 caaggtgcct gattattttc aatttttgta aacattccat tgagctctgc cggtgcctgt
     8161 gattgttcca taactaaata tcttctcata tcaataatta ttgaaagcgg atcaatattt
     8221 acaggacatg cctctgtaca ggcattgcag gttgtgcaag cccaaagttc ttcaggagaa
     8281 atataatcat ttaacagtgc tttattatcg tcatggtcca tgccgaactt gtcaatattt
     8341 tttcctactt cgtcaaggcg gtcacgggtg tccatcatta tttttcttgg cgacaataat
     8401 ttgcctgtta tatttgccgg gcaaacagat gtgcatcgtc cacattcggt acaggtatag
     8461 gcatccatca gttgtttcca gctaagatca cgcacatctc ttgcaccaaa acgcatagga
     8521 gcatcacttg gtggaggagg gggtaatgat ggatccatca tcagtttaac ctcattggta
     8581 acactctcta gattggttag tctgcctttt ggatttaaat tgctgtaata ggtgttagga
     8641 aatgccatca ggatatgaaa gtgcttagag aagggcaggt agttcataaa tgcaagtatg
     8701 ccgatgatgt gtgcccacca gcagagcctt tcaataaaaa ttaatgttga atcaggcatg
     8761 ccgataaata aattacttaa tgaactagaa actggaaagg aacctgcttt tatataatgc
     8821 gctgcacctg attgttgcaa cagatagtcg caggcattca tagtcagaaa tagcgacatt
     8881 aatataattt cagtaataag aataatgttg gcatcacttc gtggccatga ggtcatttcc
     8941 ttactccaaa aacgttttag tttcagaata ttccttcttg ccagaaacac aatacaagct
     9001 attattacca acactgccag aatctcgaac gaaccaatga gaaaatcata aaacccaccc
     9061 ataaaagata aaatcctgtg tgtgccaaaa attccatcaa tcattatttc cagtacttca
     9121 atattgatga tgataaatcc ggcataaact atcaagtgga aaaatgcagc taccggccgg
     9181 acggtcattt tgctctgacc caaggcaaca cgaatcatgg tcatccatcg ctgagaagaa
     9241 ttgtccgatc tgtcaagagg tttgcctaaa aaaatatttc gcctgatgcg tcttatgctc
     9301 tttccgaaaa agaatattcc ggtagccagc actatggtaa atattattcc ctgaatcatg
     9361 tattagctta aatcaattga tgcaaatgta aaataaacca aacaatttat ttgagattta
     9421 gtattatctt tcgttcgtga aaaatagcct gcttttttta ttgatttatt tctttccttc
     9481 tttttacttc tactcggaga gtttatgttt tgatggtaat gctatggcaa tgcctactga
     9541 aagagcagat tcacttcata ttaagttggc aagagccaag gaggataaaa ccaaggttct
     9601 gatatattgt gacttaatgg ctgaatatat agacaacaat ctggatactg catttatgtt
     9661 tttgaatcct gcattacaat tggccgaaaa aatcagatgg gatgaaggaa ttgccagagt
     9721 aaagattgaa ggcggccgcc ttttttggcg aaaaggaagt tttgaaacag cattaaagta
     9781 tcataatgat gctttagctt atttcattaa agagaataat aaagatagta ttgcagagat
     9841 aataactttt attgggcagg attatgccga ttggggaaaa taccaggaag cattagagta
     9901 ttttgtcaag gctgattcta tttatcgtca aaccggtaat gaaaaaaaat tagggggaat
     9961 ctatttgttg tatagcttta tttatataaa ccagggaaac tacacagaag ctttaaaagt
    10021 caattataag gctctgaaaa tatttgaagc taatcacgac cagtatggtg cagcaattac
    10081 cttaatgaac attgcagatt cctataaaag cttaggtaat tatcaggatg ctctaaagag
    10141 ttattttaat ggtgtaaaga tttttgaaaa agacattgat aaatttaatc ttgcagaaac
    10201 atattccagt attgctgaaa tttatatatt aataggcaac taccaagaag cactaaagta
    10261 taatagaaaa gcattggcga ttgctaaagg agttaataac ggttacctga ttgcttctgc
    10321 cacacaaagc attggcgaag tttattttca acagcaaaat tataaggaag ctcttggcta
    10381 ttttataact tcagcagaat cttacagaag tgtttcagat tatttagacc tttcattaat
    10441 actaagtaag gttggtcttt gtcaggtcaa acttgaaaac tatgaaggtg caaagaagaa
    10501 tttggatgat gcttattcaa tagcacagac acttgattca agacttacct taacggcata
    10561 ctatcaggga gcagaacttt tagacagtgc tacagggaat tggcaagggg catataataa
    10621 tttcaaggag tatatcagca accgtgacag ttcagtaaat ttagagaaca cgaagaaaat
    10681 attgcagcaa caaattcaat acgcaagtga taaaaaagat gcattgctcc aagcagaaca
    10741 agacaagaag gatgcagtgc aaaaagtatt tcgttattca atgctggctg gtgctttaac
    10801 attattggtt ttattgatag tagtttacaa ccgttatcag atcaagacta aaaccaatgc
    10861 aaaattacag gaggcatatg atgatctgca gaaaacgcaa cttcaattgg ttcagcagga
    10921 gaaaatggct tcattgggtg cattaactgc aggtattgca catgaaataa aaaacccgct
    10981 taattttgta aataattatt cagaagtgag tcatgagttg attgatgagt ttcttggatc
    11041 taaagatgaa aaggagagag aggagattgc acaaatttta aaacaaaact tggagaaaat
    11101 tgcagagcaa ggcaagcgtg cagacagcat tgtaaagaat atgcttaaac attcccgcac
    11161 cggaagtaca gaaaaacaac ttacagatat aaaccttatt tgtgatgagt atcttgatct
    11221 tgcatttcat ggtatgcgcg ccaatacttt aggtttcagg tgtgccttgg tgaagaactt
    11281 tgatcctgat ttaccgaaag taaattgtgt gggacaggat atttctcgtg ttattctcaa
    11341 tctgattaac aatgcatttg atgaggttta tgaatgtgat caacttggct tgaaaggtgg
    11401 agatgagtat cagccaacta ttacggttac tactgcggtc tcaagtcaac ttacaaataa
    11461 atcagggact aagtatgtta caattactgt tggtgacaat ggcagaggag tccctgatca
    11521 tgtaagacaa agaatatttg aacctttttt tactaccaaa cctacaggaa aaggtacagg
    11581 tttgggattg agtttaagtt atgaaataat tgaatcgcat ggtggcaaaa tagacataga
    11641 ttctaaagcg ggcgaaggga cgaattttgt gatttcacta ccttgctgaa aatttgcaac
    11701 aattatttta ttgtaaaatt gaatctggat ttttttgaaa gcaatacatt ctaaccactg
    11761 gcaaactgaa agcactttat actcaatatg cagaagtttt ttttagtgat ttgccaatac
    11821 atgataaatg ttacttctcg ttttaattta aagcggtatt tttgagcaga tttttattga
    11881 ggatgaaaaa tattaaaata ggaatactaa gagaagaaaa agttccacac gataaacgcg
    11941 taccattcag ccctgaacaa tgcaagttaa ttaagtcaga atatcccggt gttgaggtag
    12001 tgattcaacc atgcagctat cgctctttca gggatgatga gtataggaga gaaggagttg
    12061 ttttgcagga agatttatca gattgcgata tacttattgg catcaaagaa gtgccaaaag
    12121 ataaactcat tgcaggaaag aaatatttat acttttcgca taccataaaa aagcagccac
    12181 ataaccgtga aatgctgaag aaaattattc gagataaaat tacactcgtt gactatgaat
    12241 gcttagttga tgatcatgaa aaccgcatta ttggctttgg ccgctatgcc ggtattgtag
    12301 gagcctataa tggcatcatg gcttatggtt taaagtatgg cctgtttaat ttgaaggcag
    12361 cacatcagag ttttggtatt gaagaactgc ataaggagtt agacagtata aaacttccga
    12421 atattaaaat agttattacc ggaggtggtc gtgttgccaa tggtgctatt gagactatgg
    12481 gaatgctgaa tatcagaaaa gttactccat ttgaattttt gaattattca ttccgtgagc
    12541 cggtttatgt gcagctgcat tctgaaaatt attacgaacg taaagatgga tctgattttt
    12601 cgaaagccga cttctatcac tatcctgaga aatataattg tacattttgt gacccgggat
    12661 catattcttc ttttactgat ttattggtgc attgtgccta ttggaatcct aatgctgctg
    12721 tgctgtttac taaggaagat atgcgtagcc cggcattccg tataagtgtc attgcagatg
    12781 taacttgcga tattgatggc agtattcctt caacgcatcg ggcaacaact attgaggata
    12841 agttttatgg ctataatccg gtgaatgaga aggaagaaga ggcatttagt accgatacca
    12901 taacggtaat gtctattgat aatcttcctt gtgaattacc tcgtgatgca tcagaaggat
    12961 ttggtaagca cttaattgaa cgtgtaattc cgcttttgat aggtcatgac aaagacaatg
    13021 tgatttatcg tgctaccata gctcgcaacg gtgaattgac tccacgattt gcatatctta
    13081 cagattacat cagttgatgt ttaccctttt ttaagtactt caatagttgc ctttgggtca
    13141 gaagagctaa aaacggtatt accggcaaca agtacatcgg caccggcagc tataagtttt
    13201 ttatagttgt tcaggtcaac gccaccatca atttctataa gtgtttttga gttgtgaatg
    13261 gcaattagtt ctttcaactc ctgaattttt ctgtaagtgt tttcaataaa tttctgaccg
    13321 ccaaaacctg gatttacact catcatacaa attaaatcgg cttcaaaaac aatatcactt
    13381 aatgaggcta caggtgtgtg tggatttatg gcaatacctg ctttcatacc ttcttttttt
    13441 atctgctgta atgtccggtg cagatgtgtt gatgcttcta tatgcacggt taaaacatca
    13501 gcacctgctt cacgaaactg ttcaacgtat cgctccggtt ttacaatcat gagatgaaca
    13561 tccaaaggct tttcggcata ttttttaata gcttttatca ccggaaatcc aaatgaaatg
    13621 tttggcacaa atacgccatc cataacatct acatgaaacc aatcagcatt acttttattt
    13681 atcattgcca catcactact taattgtgaa aaatcggcag aaagaattga tggagcaatc
    13741 agatgcatga tgtgaatgtt ttgacaaagc taaccaatat ccataaaaaa agagcctgac
    13801 ggctctcttt ttaaaaattc aaatcaaata actattggga ctaattcatt tttgaaggca
    13861 caatcattct aaattcaggt atcttaacat aaaacatttt attatcgcta aggcgttcca
    13921 ttagataggt gccatacatt tttcctattt cagtattcag gttgcatccc gaaacatagc
    13981 ggtatagctg acccggttca ataacaggtt gttcaccaac aacaccctcg ccctcaactt
    14041 cgcgtctggc attatcgctg tcaatgatat accaatgtct gcgttttagc ttaatgcttt
    14101 cttcgctttc gttagatatt gtaatccgat aggcgaatac ataatgatgc tgaacagggt
    14161 tggagtgggt gggttgaaag aatgtttcaa ccgttacctt tattcccgct gttacctctg
    14221 ttgacatata catgatgtgt tgttgtgaat aacggatgta aaattaaaca tgcctttatg
    14281 caacttcggt ttgttttggt aaaagtactt ttttacaggg cgaaggttaa gtttatctat
    14341 ataggcatta gaatagcagc aatgttttta ggtaaaatgc agtgaattag tgttaaaaat
    14401 gaaggataaa agctgatttt atcttgattt taaaggttgg atgattcttt aggcaaataa
    14461 aaaagcggaa tcacccgctt ttttgtaatt gaatattcta atttatcaat tttatcgtgc
    14521 atctcttctg tctttaagct gacgaagtaa ttctttctta aaatcttctt ctgcctgata
    14581 aagcatggca acttttttaa tgggcaacac ttgtttaaat tgagcatgat attttttcaa
    14641 aatatccaat tcactttggc ggaaaagtat ttcatcgtcc acaacttttt ctgtttcttt
    14701 atcagacaac tgatcaaagt cttgtctggc atcacgtctg ttgcctcttc gtttttgtcg
    14761 cagcgagctt aactcgtcct gaaattgatt ataaaccggc cagaattttt ttgcttcgtc
    14821 ctgagttagg ttcaatctgt tggtgatgaa tccaattttc attgcttcag ccttttccct
    14881 tttttctatc ttcttttgac cgggttgtgc cattgccata cctgcaaaaa gtaaaataag
    14941 cacagtagct cctgctgatt ttattaattt aaatgttgtt gatatcataa cttaagtcat
    15001 tatagttttc aataagataa tttattgttt cagcatcagc attgttatct gccggtacaa
    15061 tagattctaa ctgatcggta tctagtaagt cactcagttc attgacattt aaagagtcaa
    15121 catttacagc tacaaatgca tattgattat ctgctttgtg ttgattgaac atatataaac
    15181 ttccaaccaa aagtattaca atcagaccga ctgcaaattg cggtttaagt aatcctgaaa
    15241 gtaaagtttt aattatattg gtttttggct gcttaatttt ttgttgaatg gttgagggta
    15301 gctgatcaaa ataattttca ggaatatcaa aaagatgctt tttttctatt gacgacagca
    15361 gcggagcttt atccttcaat tcattataaa tttcatcttt catactactt tgatgttatt
    15421 aatttaaaaa ggtttaaaat gactgtaaga atttttcaat tttttgcgct gcatggtgat
    15481 aagatgcttt aagtgcacca actgatgtgc ctaaaacctc gctcatgtct tcgtatttca
    15541 tttcatcata gtatctgaga ttaaatacaa ctctctgttt ttccggtaaa gataagatag
    15601 ctttttgaag tttgagttcc aactcattgg cttttaaaga tggttcatgt tcaagtttgt
    15661 ctgccaattc agcttcaaca tcatggatag gaataaaata gcgtttcctt ttgtttttca
    15721 gaaaagtgta acattcattg gtagcaattc tatacagcca tgtataaagt tgtgcatcgg
    15781 ccctgaaatt tcccaaagct ttccatgctt ttataaatgt tgtttgcgtt aaatcatcag
    15841 catcattatg gtcagcaacc aaacgcctga catgagtata aatcttctgt tgatattttc
    15901 taacaatgag gttaaatgcg taatcggcat tgccgttact aaattgttca agtagttctt
    15961 tatctgagta gtgattcaat gaaaaaccga tttatcctat tacttcaatt taaaaagatt
    16021 cccggagttt cactgggaaa aaattagcct gctaattcct ttttgcgttg taaaacctgc
    16081 tctactgcaa agacaatatt cttactgtcg agtccgtatt ttgtcatcag cgcatcaggg
    16141 gttccacttt caccgaatgt gtctttcact cctaccattt caacaggtga gggtaaatga
    16201 cgtgacaata actgacaaat actatctcca agccctccat taatctgatg ttcttctgcc
    16261 gttattgcac atccggtttt tgaaactgat tgtatgactg ctttttcatc aagaggttta
    16321 atggtgtgaa tatttattat ttctgcacta atgcccatgt cggcaagtgc cattccggct
    16381 tctatggctt tccaaaccaa atgacctgtt gcaaaaatac tgacatcact tccctcattc
    16441 aacagcaatg ctttaccaat ttcaaaaggg gtgttttcag gaataaatat cggccacgat
    16501 ggtcgtccaa aacgcaaata aactggtcca atatgttttg caatagcaat agtggctgcc
    16561 ttggtttgat tataatcgca aggattaatt acagtcatac cgggcaacat tttcatcaga
    16621 ccaatgtctt caagtatctg atgtgttgcg ccatcttcgc ccaatgttaa acctgcatgc
    16681 gaggcacaga tcttaacatt tttacctgaa taggcaattg attgccttat ctgatcataa
    16741 actcttccgg ttgaaaaatt tgcaaacgtt ccggtaaaag gaatttttcc gccaatagtc
    16801 atacctgctg ctatgcccat catgttggct tctgcaatac cgcattgaaa aaaacgatcg
    16861 ggaaaagctt tctcaaaagc atccattttt aacgaacctg taaggtcagc acaaagtgct
    16921 acaatattgg ggttgctctt acctgcttct aacaatcctg cgccaaagcc tgaacgggta
    16981 tctttttttt cggtgtaggt aaattttttc attactcaat tatttgttga tgttgactgt
    17041 aattcctgat ccggaagtta tccgaaatgt tttttctact gtataaattg cttctttcga
    17101 aatttttggt ctgaatacta ctctgtagtt tccgggttgt agtaatatac tttcatgggt
    17161 gttgttttcg ttgagattac aaacccaatt cagtttattg tctttttcct gatacacact
    17221 tccataaccg gcagaaacag tttggaaagt tgccatgccc ggatttggaa tctgaacggt
    17281 tgtggtttta ctctgtgcaa tatttacatc gttgataagc atccttggca gacataaaac
    17341 ttcaaggtca taatctcctg tgaggtagcg tgtagtagtg ttaaatgcct gaacatgcag
    17401 tgttttactg tcaccttttt ttctgacaat gcattgaagg tttttatagt cgcttgtgcc
    17461 gtcaaacttt aagaataaat caccctgagg gcaatcaatg ccaatagtat tgtgttttcc
    17521 tgcaacaatt ctgttgctgt cttttctaac cggaggtatg gtgtttacaa caatatcgta
    17581 ggtacccaaa gggtcaataa gtaacgtgtc cggtagtcct tttgcattta atgtatggac
    17641 aaaattataa cgtagggcac ccgagtaggt gtcataaaag gtcatattta cattggtttc
    17701 tgtcggtctg tgcttttcat caagcaggtt aacctggata gttgtgttgt ttaatgcttg
    17761 tgaaataact acattgaata ctgtacggaa tcctgtttca tcaactgcat cataatagtt
    17821 acctacacaa ttaaaaacat ctttatactc tttggaaagt ccaagaccga taacaaatgg
    17881 tttcagaata attccttttt gctgcaacat gagtgatact gcacaggggt caccattaca
    17941 ttcttctatt ccatcagtga taagaataat aatatttcgt gcaggatcag cagggaagtc
    18001 attgccacat tgattgagtg attgtgctat tggagtggtg ccactcggac ttatttcact
    18061 cagtcttttt ttaatcagat aggctgtatt ttttccaaac ggaacttcaa gtcttgtatc
    18121 gtcacagtcc tgaggaggaa attttttggt gtgaccatag acacgcaaag ccatttcaac
    18181 attggtgatt ttatccaagc tgtcaaccat ttcagaaagt agctttttgg caatttcaaa
    18241 acgggtattt gtttgccatt gtgcatacat gctctgtgat gcatcaaata aaaactcaat
    18301 gcgtgttaga ggtaaagaat tgtttttttg tgccttaacg gttgaggtaa agactataaa
    18361 aaaagctatg aggaaaaata gtttcctgaa attatgcaca caacttttta taaccaacat
    18421 acaatcaata atcaccaata gtttccggca gttgtgccag tgcttgtttc aactgttcat
    18481 cattgggtgc tatgccatgc catttgtgtg atcccatcat aaagtcaaca ccataaccca
    18541 taacagtagt catcagtata acgacaggtt taccttttcc tgtatgttta agtgcatttt
    18601 ggagtgtttc aacaacttgc ttcatgtcgt ttccattcat ttgcagtaca tcccatccaa
    18661 atgcctccca ttttgctttt aggttgccca atgaaagcac atcatttaca gcgccatcaa
    18721 tctgctgccc gttataatca actgttgcaa taaggttatc aattttttta gcagcagcat
    18781 acatcattgc ttcccaaatc tgtccttcct gtaattcacc atcaccatgc agggaataaa
    18841 caatagaact atcgttattt aatttttttg attgcgctgc gccaattgca acactaagtc
    18901 cctggccaag cgaaccagac gacatacgaa caccgggcag cccttcatgt gttgttgggt
    18961 gaccttgtaa acgactgttg atactacgga atgttgataa ttctttttta tcaaagtaac
    19021 cgcttctggc taatacactg taccacaaag gggaaatgtg gccatttgaa aggaagaaga
    19081 tgtcctcatt tttcccatcc atgctaaaag ctgtgggatt gtgtttcatg attacgaaat
    19141 aaagggcagt tagaaaatcg gcacagccta aagaaccacc ggggtgtccg ctttggtggg
    19201 catgtaccat gcgaacaata tcgcgtctga cttgtgtgct gattttaata agctgttctg
    19261 ttgtgggcat gagctaaaaa ttatgctcca aaactcggta aaatgcttgt gtaacaaaaa
    19321 ggaagttttt aacaaaaaag catttgttat ggaatgtatg atgataaaaa tagctgtgaa
    19381 atcagttaag gcagtagagt aataaaaatt ttgtagaaaa tatataccta cacaaccacc
    19441 atatttatgg aatggttaaa aaattagcct gttaagactg ataaatttaa aattatccta
    19501 agtaaataag aaagaataaa aaggcaaaaa ccaaaatcat ccatacggca gtaaatgcgc
    19561 cagtgccgcc ttcatataaa aaagtctgtc ctttttcttc tacgtggtga tgtcccattg
    19621 ttttaaataa tttttgcaaa gataattctc tggaacttaa ttcgcaatat tttgataccg
    19681 caaatttttt gtcctgccat tagaagtgtg ttgataaaaa tcagtcagat caattgttga
    19741 aatatactgc gtcttgaatt gcaattggtt ttcgtaatct ctacatttgc gcatcaatta
    19801 aaaactaatt taaattaata gctatgtcgt caaatagtgt acttagcctg tttaaatcat
    19861 ccattggtaa gaaagttatc atggcgctga caggactttt tttaagtata ttcctggtgg
    19921 ttcacgttag tggaaatttc cagttgttta aaacagattc aggtctttct tttaaccagt
    19981 attcggtgtt aatgactaca aaccctttca ttaaaaccat atcttatctg ttgtacatca
    20041 caatagtttt acatgccata agaggaatta tgctggtgct tgaaaataaa aaggcacgcc
    20101 ctgtatctta tgccatgtat gatggaaagg ctaatagcaa atgggcatca cgaaatatgg
    20161 gtttgttggg tactgtaatt ttatttttta tcgttatcca tctgaatgat ttttggtatc
    20221 agtataaatt cggtcatata ccatttacca tgtataaaac agaacttact acaggaaaat
    20281 tggttgaagt aaaagaaatg gaagccggat ttaagatggg ttcaaaaatg gaagagatga
    20341 ttgttggcga ccataaatat gtaattgtaa aggatttata caaagaggtt aaagatgagt
    20401 ttaaagaatg gtggatggta cttatttatg ttgtaagtat ggcagctgtt tcattccatc
    20461 tatatcatgg atttaaaagt tcatttcaat cgttaggact caatcacaga aaatacaacg
    20521 gattaataca gtcaataggc acatgggttt ttgccattat tattcctgca agttttgcag
    20581 ctatgccgct atattttttt attaaccgat aatacttaat gaaaattatg acaaacaaca
    20641 tacttgacgc acatattcct gccggtgata tagaaaagaa atggagcaaa taccggtcaa
    20701 cggtgccatt gattaatcct gccaataaga gaaaccttga aatcattgtc ataggctctg
    20761 gtcttgcagg tgcttcagca gcagcttcgc ttgctgagtt aggatataaa gtaaaagttt
    20821 tttgctttca ggattcagca cgaagggcac atagtattgc tgcacaagga gggatcaatg
    20881 cggcaaagaa ctatcagaat gatggcgact caatttatcg cttattttat gatacagtta
    20941 aaggcggtga ttaccgttcg cgcgaagcca acgtatatcg ccttgccgaa gtaagcggta
    21001 atattattga ccaatgtgtg gcacaaggag ttccttttgc ccgtgaatat ggtggcttgt
    21061 tgagtaacag atcttttggt ggggtacagg tgcaacgaac attttatgct gccggtcaaa
    21121 ccgggcaaca gttacttctg ggagcttata gcgcattgat gcggcaggtt ggcatgggtg
    21181 ctgtaaccat gtattcacga catgaaatgc ttgatattgt aaagatagac ggtaagtgcc
    21241 ggggtattat tgcacgtaat ttaattacag gagaattaga aagacatttc ggtcatgcag
    21301 tactgatgtg tagcggaggt tatggtaacg tgttttatct ttcgaccaat gcaatgggaa
    21361 gcaacgtaac tgcagcatgg aaagcccata aaaatggggc ttattttgcc aacccttgtt
    21421 tcacacaaat acacccgact tgtattccag tttctggtga acatcagtcg aagcttacac
    21481 tgatgagcga gtcactgaga aatgacggac gtatttgggt tccaaagaaa aaagaagatg
    21541 cggaagccat tcgtgcgggt aaattaaatc caaatgactt aaaagaagaa gagagagatt
    21601 attatctgga aagaagatat cctgccttcg gtaatttggt gccaagagac gttgcctcgc
    21661 gtgctgcaaa agagcgttgc gatgccggct ttggtgttaa tgctacaggt gaagctgttt
    21721 accttgactt tgcttataac atgaagtata agtatggtaa aatggaagct actatgaata
    21781 gcattgagaa tcctacagaa gagcaacttt ttgatttagg taaagcagtt gtaaaagaaa
    21841 aatacggtaa cctttttgat atgtataagc agattaccgg tgtagatcct tatcataacc
    21901 cgatgatgat ttaccctgct gtacactata caatgggagg attgtgggtt gattataatc
    21961 tgatgaccac cgtaccgggc ctttacgcac ttggtgaagc taatttcagt gaccatggtg
    22021 ccaatcgttt gggtgcatct gcactgatgc aaggccttgc tgatggatat tttgtgattc
    22081 cttataccat tggagcttat ctcagcagcg aaatcagaac aaaagctatc agcactgacc
    22141 atgaagagtt tgttgctgca gaaaatcgtg tcagagaaac tttgcaaaga ctcatgcaga
    22201 taaaaggaac aaaatctgtt gaccattttc atcgccagct tggaaagatt atgtgggaga
    22261 aatgtggaat ggctcgtaat gcaattgcac taaaagaagc tataacagaa atacaagagc
    22321 ttagaaaaca attctgggca gatgttcgta ttccgggtga aatgaatgag ttcaaccctg
    22381 aacttgagaa agccggcaga gtagcagatt ttcttgaatt agctgagttg atgtgtatgg
    22441 atgccttaca gcgtaatgaa tcatgcggag gacacttcag agaagaacat cagacagaag
    22501 aaggtgaagc tttgcgtgat gatgacaact ttgcatttgc agcagcgtgg gaaatgaccg
    22561 gtgatgcaca gtggaaaatg aataaggaag agttgaaatt tgaaaacatt aaaattgcac
    22621 aacgtagtta taaataatat tttatcagaa aaatttaaaa taatacaggt atgaatatca
    22681 ccctgaaagt ttggaagcaa agaaacaaag atgagagagg tcgttttgaa acctatcagg
    22741 tacaggatgt ttctgaagac atgtcttttc tcgaaatgtt tgatgtactg aatgagcggc
    22801 ttattaacga aggaaaagat cctgttgcat ttgaccatga ctgccgagaa ggaatttgtg
    22861 gtgcttgcag tatgtatatt aacggtcgtc ctcatggacc attacaaggt gtaacaacct
    22921 gtcagttgca catgaggtct tttaaagatg gtgaaacaat tgttgttgag ccatggcgct
    22981 cagcagcatt tcctgttatt aaggatttgg ctgttgaccg ttcatcattc gacagaatta
    23041 tggctgccgg tggttttgtg tcggtgaata caggaaatgc acaggatgca aacagcattt
    23101 tgataccgaa ggacgatgct gacaaagcat ttgaagccgc tgcatgtatt ggctgtggag
    23161 cttgtgtggc agcatgtaaa aattcttctg caatgctatt cgtttctgca aaagtatctc
    23221 agctttcatt gttgcctcaa ggtaaggcag aaaggcatga gcgtgtaatg aaaatggtag
    23281 cacaaatgga tgaggaaggt tttggtaatt gtacaaacac cggtgcctgc gaagcagagt
    23341 gtcctaaaga aatttctatt gagaatattg cccggatgaa cagggaatat ctttcagcaa
    23401 aaatttctta aacaacatct attccctgaa ctttttttag cagcattcgt aaaagcaaac
    23461 gaaagggtag tttattaccg gatggttctt tttctaagaa tcatttttaa aattaaatag
    23521 catgacagca tcagaagaag aaagaataag aaaagcattt tcagataaag actggcagga
    23581 aattaaagct caggatactt ggcagatttt taaaataatg agtgagtttg ttgaagggtt
    23641 tgagaaaatg agccgtattg gcccttgtgt ttcaatattt ggttcggcaa gaacaaaacc
    23701 tgacacaaga tattatttat tggcagaaga aatagcgtat caactcacac aatccggtta
    23761 tggtgtaata acgggtggag gcccgggtat tatggaagca ggtaacaaag gcgcacaacg
    23821 tgccggtgga aaatctgttg gcctgaatat taaattgcct tttgaacaga catcaaatcc
    23881 ttttgtagat catgataaaa atctgatgtt tgattacttc tttgtacgca aggtaatgtt
    23941 taccaaatat gcacaaggtt ttgttgtact tcccggtgga tttggcacgt tagacgaatt
    24001 ttttgaagca ttaacactca ttcaaacaaa aaagattggc agatttccaa ttgtacttgt
    24061 tggcaaagag tattggagta aattgtttga atggctgcaa acagttatgg ttgatgaagg
    24121 caatatttta cctgaagacc tttcactctt taagttagtt gatgaagcgc atgaggcaaa
    24181 gtcgcacatt gacgcattct acagtaaata cttattgaag cctaactttt aatccctacg
    24241 ccactcactg caaaaaattg cagcagacat ggcaatattt aaagattcaa cagggctgct
    24301 tttttcatca ggaaattttg gaatggatat agcctgatca attaattgct gaattgactg
    24361 tgaaacacct cttgattcat ttccaaaaac caaaacagca ggccttttct tagcgacagc
    24421 ataaacagac ttaccattta gcaatgtagc ataaaaagtg gcgttttgtt tattagcaga
    24481 aacccatttt tcaagatcag tataaaaaac attcactctg aaaatggatc ccatggtagc
    24541 ctgaatagtc ttgggattat aaagatcaac agacgttgat gaacaaacaa tttgtttaat
    24601 accaaaccaa tcagcaattc tgataatact acctaaattt cccgggtcac ttatgtcatc
    24661 aaatgccaaa atgatgtctt taaattgtat ttcggtttcc ggttgttgcg gcatggccac
    24721 taaagccaac acgctttgag gttgctttaa cgaagatatc cgctctaatt cttttgtttt
    24781 aactttaatt agtttttctt ttaataatcc ttttatatca ttgcttgact gttcaatcca
    24841 ttcatcagta gcatataccg ctatgatagt aaaggctgaa tgggccagtt ctgcaaccat
    24901 tttttcacct tcaacaacaa atttttgttg ctcatggcgg ttagccttgt cctttagtga
    24961 ctttatctgt tttatttctg aacttgtcag cattagaaga atactgttaa agggtataaa
    25021 gatagatgat caataagttt tatttttacc gcatccgaaa aattatgcgc aaaaggcagt
    25081 acggcatttt atttactctg attttactgg tggcatcttg cagccccttt aagcaattgg
    25141 acaaaggaga gtatttgctg aatcgcaatc aggtaataat agacaagccg gaactgcgcg
    25201 aaggcgttaa agcaattatc aaacaaaaag ctaataggag aattttagga ttgttccgtt
    25261 ttcatcttgc tgtatatagt atgggaaatc ggggtaaacc aactaagttt aaaaattggt
    25321 taaagaacac agtaggagag gagcctgaag tgttggatac actttttact aagaaatcta
    25381 tacagcagat aaagcaattt atgtctaaga atggctattt tgatgcagaa gttaaagaca
    25441 gtaccattta cagaaagaaa aaggcaattg tccgatacac tatcattgca cataaaccat
    25501 cgtttataaa atatgtggct cgtgaatgtg ctgatgacaa cctcgactct cttgttttaa
    25561 aagatacttc aacatcaaaa ctgcaagcag gaatcattta taatgaaggt atattgcaag
    25621 gcgaccgcga acgactaaca aaaaagcttc gcaatgaagg atattttcaa ttttcaccac
    25681 agtttgtttc ctatcgtgtt tacacatcag atactacaaa gaatatagcc gtctttcagg
    25741 atgtagcagc cttttcaaac cccgattcaa cagtaagttc cgttgcaatg ccacatacaa
    25801 agtttaaatt gaaccggata atttttagtc ccgactatga tcctttgagt cttaatgcat
    25861 caatgattca atatgataca gtagcttata aaggttattt gtttgtgaag cgggcaggaa
    25921 agaaaatgcc tgtgaagcct gaagtaatcg ttaaccatac atttcttaag cctgatgctg
    25981 ttttcaggca gagtaatgca gactatactt ataaggggct aaatctgctc ggcatgtatc
    26041 gttttgtgaa tatcaactac aatgcagttg acagcaataa acttgattgc tttataaatc
    26101 ttggtgcaac gcctaagcaa gaatataaga cagagtttga agggaccaat aacggaggca
    26161 accttggtgt tgcaggtgat tttacttata gaaacaaaaa tgtttttaga ggtgcagaga
    26221 tgtttgagtt taaaatcaga ggggcattag aatcgcagaa aaaatttact gatgacaaag
    26281 acaataaagt attgttgtta ttcaatacct atgaactggg agtagagaat tcaatatacc
    26341 tgcctaaggc cataaaacca tttaaccgtt tgtttaaaaa tattgctgcc aatcctgtca
    26401 ccgtattttc taccaattat aatattcaaa accgcccgga gtttaatcgt tctattctcg
    26461 atttttctgc atcattagaa tggcgaaaag gtaggtttat caaacaaatc gtaacaccat
    26521 ttcaaataaa ttttgttaat gtaaagcttg aacctgaatt taaggcgaga ttggaaagtc
    26581 tgaacgaccc tgtgttgttc agcagttatg acaaccacct gatttcaaat ggccgataca
    26641 ccctcatcta taacaatcag caattgaatg agcccggtaa ttttaaattt ttcagaacaa
    26701 atattgaaat tgccggaaat acactttact ggttcaggca aatagaaggt gcagataagg
    26761 atgtgaacaa cagttataca attcttaaca aaaggtttgc tcaatacttc aggcctgatg
    26821 ccgatttcag attttatcaa aatataaatg cacataattc tattgtgtat agaattgctg
    26881 caggtattgg tattgcttat ggtaattcaa aaataatccc gttcgaaaaa agtttttatg
    26941 ccggtggatc gaacgattta cgcgcatttc gtgctcgtaa tgttggtccc ggaaatttta
    27001 ccagtgtaaa taattttgaa cgtacgggtg acataaaaat caatggtaat attgaatatc
    27061 gtttcgatat tttgaaaatt ctgaagggtg cattctttct tgatgccgga aatatctggg
    27121 tgaggaaaaa agaaacttca cgtcctgatg ctgagtttga gttagatcgt ttttataagc
    27181 agttagctgt tggtagtggg ttaggtttcc ggtttgactt taccttcttt attttcagac
    27241 ttgatattgg tgtacctatt gttgacccac aatataaaag tgatgacaaa gtgattgttg
    27301 atgaagtaaa accacgcagc ctgatttata atttcggtat aggttatcct ttttaataga
    27361 ttttaccagt caagaaaatc atctaatgcg cgtctttctt ttttagttgg ccgtccctca
    27421 ttgtgctgtc tgtaggtgcg cacagcaaga ctgtgcatct tgattttttc atactcatcg
    27481 gctgatgtca ggtctttgca aaattgatct ttaattgccg ctgacactct gttttctatt
    27541 ggttgaataa cctgaaacac taatgtaaaa gaaccacgcc tgattgataa tatttcattg
    27601 gttttaatta gtcgtgatgc ttttacagaa acaccatcaa taaatatttt tcctcttgca
    27661 caggcttcag cagcttgcga acgtgttttg aaaattcgaa ctgcccacat ccatttatca
    27721 atgcgtactt tttcaagttg aatattcata cttaaaatta aaatcgttaa aaatattatg
    27781 gttgctgaat ccctttcatc gaaaactgaa ttcttcctgt tgtaatatct gcacttaaca
    27841 cttttaccat cacctgttga tttagtcgca ctgccgtaga tatatcagaa ataaatgcat
    27901 cactgatttc cgatatgtga accagtccgt cttttttaat tcctatatct acaaatgctc
    27961 caaagtttgt aatgtttgta acaattccgg gaagcaacat gtcaggctgt aagtcattga
    28021 tgctttttat ttcagcatta aattcgaaga cttttataat tccccttaca tcccgtccgg
    28081 gttttgataa ttcagcaatg atatctttca gcgtaggtaa cccaacttca tctgtcacat
    28141 attttgtaat atcaatcttc tgaatcaggc tttcgttgcc tattaaaccg gttatggttg
    28201 tgtcaatgtc ttctgccatg cgttcaacaa gagtgtagtt ttcagggtga acagcacttt
    28261 tgtctaacgg attatcaccg ttaatccgaa taaatccggc acacatttca aaggtttttt
    28321 cacccattcc tttaattttt ttcaattcac tccgggagtg aataccggaa tgtgttcgtg
    28381 catctataat tttttttgcc attacttcac caattccgga aacgtaggta agtagttttt
    28441 cgcttgcagt ttcaagatta acgcctacgg tgtttacgca gtgttctatc gtttgatcta
    28501 atgcgctgtt gagcatcatc tcatcaattt catgctgata ttgcccaata ccaagtgagc
    28561 ggggatttat ttttatgagt tcggcaagtg ggtccatcaa cctgcggcca atggaaacag
    28621 cacctcgaac agttacatca tattccggaa actctttgcg tgcttctgct gtggcagagt
    28681 aaacagaagc cccatcttca ttcaccatga agacctgtat tttgcgatca aaacgcaatc
    28741 gttggataaa agtacctgtt tcacgaccgg cagtgccgtc acctatggca atggcatcaa
    28801 ttttatattg gcttactaat gttgagattt tatttgatgc aattttctgt tggctttgcg
    28861 gtgggtgagg ataaatggtt tcattgtgta agagcctgcc gtttttatcg agacaaacaa
    28921 tcttgcatcc acttttaaat ccgggatcaa tcgctagaat atttttttgt cctaagggtg
    28981 gtgcaagtaa caattgtttc agcactacag caaacaacct agctgattct gtttcagctt
    29041 ttagtctgta atatccaata gtttcatttt ccattgaagg tataaggagt ctgttgcatg
    29101 catcttcagc agcagtttta acctgagcat ttaaataaaa gccttcggac tttatggtat
    29161 caaaaagcag attcagtaaa aatgttgagt ccggcattac agaaacccgt aacagtttct
    29221 ctttgtcacc tcttaaaatg gctaatagac gatgtgccgc acaactagtt attttttctt
    29281 tgtaatcgaa atagtctttg tatttaacag cttcttctaa tttggatttg ataacttttg
    29341 aaataataaa agatttttgt tcaaagcatt gacgaaacgt ttttcttacc tgatgatttt
    29401 ctgaaattaa ttctgcaata atgtcacgag ctccctgcaa tactttctcc gtgtctgcaa
    29461 aagtggatga aatatatttc tctgcaagtt gctttgcatt tctgtcatca cgaatgagtg
    29521 tttgagcgag tggttcaagc cctgcatctc tggcattttg tgcacgattt tttctttttt
    29581 ctttaaaggg aagataaata tcttcaagct catttaatgt tgtagctgct gcaattttat
    29641 ctttaagcaa atcgaaaaca cctttagcct taagtgtatt aatgatgaat tgctttctgt
    29701 tgttaaagtc ttcaacctct ttgagtaact tttttagctc caacaaattt tcatcgctaa
    29761 gtcctcctgt tttttctttc ctataacgag ctataaatgg tactgtagca ccctcttcaa
    29821 atagctttat ggtttgttca agctgatgaa cagaaatacc ggtgttactt ctaaaccggg
    29881 tcagtaaaag agaatcagtc aatataatgg aagagaaaac ttaaaaaatg aagttattgc
    29941 gcatagccat cattgatcca tttttcgatg gtggcaatag cttctttgct gagttgtggt
    30001 gccatacgtg gcattgcttt aacaccactt tcatgtttta tagagcctaa taatttgtgt
    30061 ttttcgcagg catctttcac gccttcataa taactcaaat caagtccatc tgcttttttt
    30121 gccggactat gacatttggt tccgcaagcg gcatctacaa taggtttaac atgaactgcg
    30181 taacttggct tatcgggaac agtaataggc gtagaactgc tggcagtagt agattttgat
    30241 gacttacagg aaggcataat taccgtagca gcaataacac aaaatgctgt ggcaactgat
    30301 aaaaatgtga atgttctttt cataattatt aaatttgttt tgcaagttta aaagattttc
    30361 ttgaattgac agacagctaa tttaattcat ctcatgtaat tttaccgaaa attcagattg
    30421 tgtcctataa accaaatgat tattacgcac gtaaagcaaa gcaggaaaac tttgcagccc
    30481 gaagtattta taagctcgag gagattgatt taaagcataa attgttcaaa caaggggata
    30541 atctacttga tttgggtgct tcacccgggt catggttgca atatgcttca aaaaaggcag
    30601 gggagaaggg taaagttttt ggtatagact taaaaccggt tgaagtgaaa ttgcccaatg
    30661 tgtttactgt tcaggataac atagaaactt ttgatttgca aactttgttt gaccgttatg
    30721 gttttaatga taagtttgat gtagtgatga gtgatatggc tccgaatact accggtagtc
    30781 gttttaccga tcaggcacgt tcgtttgact taagtatgct tgcccttcaa actgcaaaac
    30841 gctttctacg accaggcgga cattttgtgt gtaaaatatt tgatggcgaa gatgcaatgg
    30901 cctttcgcga tgaagtaaag tcatgttttg gcgaatgttt tgttttacgc cccaaaagca
    30961 cacgcaacag ttcaaaagaa atatttgtga caggaaagta ttttaaagcc tgattgtttt
    31021 agttgttgta ttaataagtt tctgaatttt aattttcaag ttatcaatca ttcactattt
    31081 ttactcaact aaaagaaata gatatgaaga gaattttact ttgtttagct tttactgttg
    31141 taggtttctt gtcatttgcg caatgcagca ttgacagcac acagactgac cccggcattt
    31201 atcccgacac tttattacct gcaacagtga atcaacctta cactcaggat atcacctttg
    31261 tgatgattac tgacactatg ggtattacaa taactaattt taacattgca aatattacag
    31321 gactgcctgt aggaatgacc tggcaatgca ataactttgt taatggatgt aactataatc
    31381 catctcagtc tatttatggt tgtgttaatt tgagcggaac accattaatt gccggtgttt
    31441 acaatgcaac agtcaccgtt attgccacac ttcaggtttt aggcgatcaa actattagct
    31501 acacattgcc atttacggtt ttacctgaca ctgttagtaa tcctggcttt tcaatgacca
    31561 atagcagtgg atgtttgcca ttgagtgtaa cgtttgtaaa caataatccc ggtcaagcat
    31621 cttatttgtg ggattttgga aatggtattc agagtaactt ggaaaatcca ccggtacaaa
    31681 tatataacgt gcctggcgat tacgtagtaa cacagtctgt tacagccaat ggttctcctc
    31741 aatattttct gactgatatt acagtagcca gcatccccga caattatggt gctccggttg
    31801 atgttcctga tatgtatttt tatctgtacg attcacaagg caatcagatt tacgacagtc
    31861 atccttctgt ttcaaataca aatgcaccat tgtcatggac gttgcccaac ataccattac
    31921 agaacagtaa ttacaccttg catgtttggg atgaagatgg cggattgttt ggtgcagatg
    31981 atgatttggg ctcagtaact tttccggggt ggagtgccag tggaacagct acaggtacat
    32041 tgtctggtgt ttccggttca ttagtagtaa attattcaat aatgcaggta cctgttgcta
    32101 caaccattca tacagatact gtacatgttt tcgcagttcc tgatacacct acggtaagtg
    32161 caaatattcc attgattttg tgtgcaggag atactgttgt tttgacttgt aatgatacgt
    32221 tggcgattca atggtatgag agcggtaatt tgttgattgg cgaaaccaat tcagacttat
    32281 ttttaacaac atcaggtaat ttctttgctg ttgtaacaaa tgcacatgga tgcacagcag
    32341 tctcagcagc aattaatgtt caggtgaacc ctaatcctcc aaagccaacg ttttttgtca
    32401 atggcaatac attaaattgc gtgttaaccg gttatatgtt acaatggtat tataatggtg
    32461 cagcaatgcc cggtgaaact tcattacaac taacagcaac gcagcaaggc acttattatg
    32521 ttgttgccac tgatagcgtt acaggctgca cttctgtatc agatccatta gtgtttactc
    32581 cggttggatt agatcaacca caatctgtta ttgcagcagt cttatttcca aaccctgcag
    32641 ataaaagtgt tgtagtgcaa cttacctcag ttagaaatat ttttacaaca attcaacttt
    32701 tagatattag cggaagggtg ttgcaacaaa ataattttac gctgcaaccg ggcactacac
    32761 agctgccatt cgatttatca gatataagta aaggagtcta tctgattaaa ataacgcagg
    32821 aaaatgcctc taaaactttg cggttgatga ttaacaggta atcgttattg cacaagattc
    32881 atttcttcga taagccattt ggctccggca aatttgtcaa caataaataa tacataacgt
    32941 atatctaatg tgatgtttct gcatttctca ggatcaaaca taatatcact cattgtgcct
    33001 tcccaaacac gatcgaaatt tattccgatt agctgacctt ttccattcag aacaggactt
    33061 cctgaattgc ctcctgttgt atgatttgag gctaagaaac atggaattat tttaccgttt
    33121 gttgcatagt tgccataatc tttcttctta tgaagttcaa gaagtctttg tgggacatta
    33181 aaatcatagt tggttgaatc gtacttttct gcaactccat caagtgttgt ataatatttg
    33241 taagcaatgc catcacgtgg ctcataacct tcaaccttgc cataggtaac gcgaagagta
    33301 aagtttgcat ccggatatat attcttttta ttctcagcag ctatctccat tagacctttc
    33361 atatacactt tttgtagatt atttatttct aatgtaatct tcgtcatttc aggaagcacc
    33421 tttaaggcat atgccaatga gcattgcttg atgagtgaat atgcagggtc gttaagaagt
    33481 ttactgagtt tgtttggagt aaacgactga ataaaattct ctgctcgttt ctgatctgca
    33541 aacacagatt tttttaagac ataatcacaa tagcttgaaa tattgttttt gtgttttttg
    33601 gcaataaaag aaagatcggc ttcaagccct gaaggcgttt tatcaatata ttctttcaat
    33661 aacattggca tgatggcttt gtcaatggca acatcataat ttttaaaata gcctgcagtt
    33721 gattttagta gattggtttt taattctgat aatgcttttt catctacagg atttgtgcta
    33781 cagatttttt caagccttga aaatgtttcg gcaaacgtga gtaattcaac acctttaatg
    33841 gcttcaaaat agtagtcggc tgcaatggat aacggccgct gcttgatatg caactgatta
    33901 agttcgctca ccactttttg atattctgtt ttagcattct tttttgccca ttgaaaaaaa
    33961 tcaaactcat gctgcgactt tacagctaaa gcattaagtc ttttcaggcc aagtatttca
    34021 ccttgccatt ttgtgtagcc gttttttata gatgaatgtt tggacgagta ttgaatacga
    34081 acggtgtcgt tatgttccat agcctgttcc atttgttcaa gccgaatttt gcggagttca
    34141 atacgcacag ggtcggtatg gttcattaaa gttcgtactg cctccattgg caaatactct
    34201 tgtgtgcgtc cgggaaaacc atataccatg gtaaaatcat tttcatttat tccatttaga
    34261 cttatgggca aaacatattt tggtttatat ggtatattct ccttactgta atcggcagga
    34321 ttgttgcttt tatctgcata aattctgaaa agagcgaaat cacctgtttg acgtggccac
    34381 atccagttat cggtttcatc tgcaaacttt ccaatgcttg atggtggtgc accaactaat
    34441 cttacatctt taaatttttc agtcacaatc agaaaatatt tatttccata ataaaaaggt
    34501 ttcacttctg catcataatg tgttccctga atagcttttt gcacaataat ctgagaaagt
    34561 tcttttatct taacagtctt ttccgtttct tttagactgt catttataaa aggtaaaata
    34621 ctttctgtaa catcttccat gcgaacaata aatgtagcag ttagccctgt gcatggtagt
    34681 tcttcgttca aattcatagc ccaaaaacca ttggtaaggt agttgtcttt aacgctgctg
    34741 tgttgaacaa cctgactgta gccgcagtga tagtttgtta gaaggagtcc gcttttaccg
    34801 ataatttcac cggtgcaacc gcctccaaac tgaacaatgg catcttttat agaggaagag
    34861 tttagtgaaa ataatgagtc aatgggtatt ttaagtcctt tgctgtacat agcatcggca
    34921 tttaaggttt ttaaaagagt aggtaaccac atgccttcat cggcctttga aaatagctga
    34981 ctaagcacta ggacagctgt aagaaatgtt cttttaaaca tgaattctga tattttatga
    35041 aggcaaattt aaaaaatgtc tgcgagtaga tttactgata aaaatcaaga tgattttaaa
    35101 tgataagtaa cctttttgaa ccagacgagt ataacagttg tctttataca atgaaaaaga
    35161 tagcccaaat agcaattatc agttctgtac tcagcacagc tggctatgca caatcagcag
    35221 agtcggatag tcacttaatt ctctcaatag tttcattacc tgcaagtcaa ataactttta
    35281 aatttgaaga aggaaaaatt ttctatgtaa aaattccttc ggaaaaattg cgtttaaaag
    35341 ttaaagttcc tgatgagcca acatttaaaa tgctgacaca tgaacagcaa cgaaaacaac
    35401 aagaagaaga agacaaaaaa tttcaggcta gacaggatgc tgtagtgcaa aatactcagg
    35461 caattgcggg caattataat cttacagata ttacattaga gcaactgcag gcacaggaga
    35521 aagaggtagt tgttgctaat gacgatttaa cagtaaaagt tgttcctttg gttgggttgc
    35581 caaccgatac aattcttcaa cagcaacaaa ttgaagaaac tgtaaaagag aataaaacag
    35641 tgcaacaaga gcttgtagga acatcagtga ttacatcgca gaaaacaatt cagaaacctg
    35701 cagcggcttt aatcaatatt agttatgctg aaacaagtga ccaggcaaca gcaaaggtgt
    35761 tagatacagg aaatggctct aaaattgaca atttaaacct gaaaagcgaa tctattccgg
    35821 tgagtatcag gcgacagcat aattctgtta tcggccgcta tattccggtt gtaattaatt
    35881 aacagttttt ctgatttatg aagaattccg tcattttcac gatttttcac cattgatttt
    35941 tgaaaattga atttgcttat tattattgca gtgttcaatg gagaaacatt aaaatggtaa
    36001 aacaagcaga gcaggttaaa caaagaaaaa agacggtatc aaatgattat catattgtac
    36061 tttataatga tgatgtaaac acatttgatt ttgtgattga atcgctaatg gaaatttgta
    36121 atcatgaaag agagcaggcc gaacaatgtt caattattgt acataacaat ggccgatgct
    36181 ctgtaaaaga tggctcagta agcgtgctgc aacccatgtg taaggcatta gttcaacgtg
    36241 ggcttacggc agaagtagaa taaattattc attcagaatt tattatttct aaacaccaaa
    36301 aacatttttt tccggttaaa taattatata ttaattctca tgaaagggac tataaaaata
    36361 ttgcttctga ttttttttgt ttcatgcagt aatgtgccgc tgacagaacg taagcaaatg
    36421 aatttacttc cggaatcgca gatggtagct atggctttaa cgtcatataa cgattttctt
    36481 aaagaacatc ccccgatggt aaatagcaat tcagatgctc aaatggtaaa gcgggtaggg
    36541 gagcgtgtaa aagatgcagt tgttcaatat atgcgcgata ataaccttta cgacagaata
    36601 aaggattata aatgggagtt taacctcgtt gacagcaaag atgtcaatgc atggtgtatg
    36661 cctggaggta aagttgtggt ttattcaggg ttattgccgg taacaaaaaa tgaagaaagt
    36721 ttagctttag tgattggtca tgaaattgca catgctattg cacgccatgg caatgaaaga
    36781 atgagtcagc aattattagc ggcaacaggc ggacttgcat tagatgttgc cttgataaat
    36841 aaacctgctg agacaagaca attgtttaat acagcatatg gtgttggtgc aaatcttggt
    36901 gttttattac cctattcacg cctacatgaa actgaagctg ataaattagg attaattttt
    36961 atggctatgg caggttataa ccctgcaatt gctcctgact tctggaaaag aatggcggca
    37021 gtttccggaa ataaagttcc tgaaatatta agtacccacc caagcaatga aacacgtatt
    37081 cacgacttga ataagtttat gcctacagca atgaagtact ataaacctcg tccttgattt
    37141 ttattaagat cacatttaac aagattctgt aagtcgcttt taatttcgat tagtgtagta
    37201 tattttctat tcaattgatt ttttacaaag ttttggtttg ttaacattta tttctcgcat
    37261 acttatcaat gggtttgata tttttaagcc gttatattct tataaaatca aaccaccatg
    37321 ttgcaatatg aatcatcatt gtttgaaagt gagtatatca aattgcgaaa cgctgaaggc
    37381 agattatatt cagatgaaat ggttgcaaag ctaccttatg ttactaagga gcatattcac
    37441 tataaagaat ggaaaataag aagagattct ttaagaaggt tgattttgtt cctgcagaaa
    37501 aatgaaaaaa ttaaacgtat tcttgaaata ggttgtggta acggttggct ttgcaatcag
    37561 ttgcataaat caggctatga agtagccggt tacgacatca atcaaataga attgagtcag
    37621 gcaaaaagac tgtttcctca tattgaattt ttcagtcagg agttgcttaa ttcccattca
    37681 gttaaaaagt atgatgttgt actttttgca gcttcattgc aatattttaa aaatgctttg
    37741 gaagtaatcc tattcagtat gaaacaattt ttgaatgata aaggctgtgt tattattgca
    37801 gatacattct tatatagtga aaatgaagat caaaaagcaa aagaaagaag cttagaatat
    37861 ttcaaaaaga acaattcaga aatgggacaa ttttattttc atcattcaac aacgatactc
    37921 aatttgtttg atgtggcaga attgaaaccg caacaatcct tgttggattt gattaccgga
    37981 tttaagaacc catttaaaat atatatcatt aaggctaaat cgtaaacatt tgagtcagat
    38041 ttgcactaaa aatacatttg aaatacgttt atcaattctt aaagttttag cttactttaa
    38101 gatatttgaa catccattaa agttaaggga aattgctgcg ctttgcaata tttctgtaca
    38161 ggaaacagag caaaatttgt cttttttaat tgaagatgag atgatttttg gttttgaaca
    38221 gtattattct acgagtagaa atgttgctca atattcagga ttaagaaagg taaaggaagc
    38281 agaggctgaa aagtatttta taaaactaaa aaagtatgca aagctgttag ctacatttcc
    38341 ctttgtgcgt ggaattgctg tttctggttc actgtcaaaa ggggttatga acccggatgg
    38401 tgatattgat tattttataa taaccaaacc tggccggctt tggatttgca ggactttaat
    38461 gattcttttt aagaagctct tcttatttaa ttctaaaaaa tatttctgcg ttaactattt
    38521 tgttgatgag aataatttgc aaataagaga taagaatatt tttacggcaa cagaatttgt
    38581 acacctaatt cctgtatttg aacacagctg tatattgaat gtttttttgt ttcaaaataa
    38641 ttgggtgcat aaatattacc cgaatttacc tgattggaac agaaattatt tggttcagtg
    38701 taatattaaa ctaaagaatg catttgagtt attattgaag acttcagttt ttaatccctt
    38761 ggagaaacta tgtcatcata ttactttata ttattggcga aagaagtttc caaactttaa
    38821 aaaagagaaa tttcatctta ccatgagaag tacgaaggga atttccaaac atcatcctag
    38881 tgactttcag acaaaagtat taagggcatt tcagattgag atgaatgaaa ttaattctta
    38941 tttaacagta tcagagctat gaaggtgtta ataacgcata gctattattt acagtttgat
    39001 aaaaagcaac ttgcgctatc taagccttat ccacctttgg caacattaat tgttgctaac
    39061 gtcttaagaa atcttggtaa cgatgtttat ttacatgatg ttagttttga ctcttctcta
    39121 cagccgttaa agaaacgaca gaaagaagtt aaccccgatc ttttattgat ttgtgatgat
    39181 gggtttaatt atctcacaaa gatgtgcctt actaatatgc gtgaagcagc tttcaattta
    39241 ttgcgttttg caaaatcaat tggggttaaa acaattatat caagtagcga ctctaccgat
    39301 cattatactg attatctgat gtcaggtgcc gatgtagtat tattaggtga acctgagtca
    39361 acattgattg aattgtttga taaaggaatt cgtgactttg aatcaatcaa aggaattgct
    39421 ttcaaaagta atggcaacgt taaatgtaat gagagaagaa aagttattac acaattagat
    39481 aaattgcctt tcccggcatg ggatttaatt gatattaaac cttacaagag ggcatgggaa
    39541 aaacataact atttttctat taatgtgtca acaaccagag gttgtccata taaatgcaat
    39601 tggtgtgcta aacctattta cggcaaccga tataacgtgc gttcgcctga acatgtattg
    39661 aacgaattga aagagatgag taggtttttt gagtttgatc atatttggtt ttgtgatgat
    39721 atatttggac taaagcctgg ctggttagat tccttttcaa aactcttaat tgaatcaggc
    39781 atgaaattaa agtacaaaat acaatgtcgt gccgatttat tgcaaaatga agagttgatt
    39841 gacaatttat caaagagtgg ggcagatgag atttggattg gagctgagtc gggttcgcag
    39901 aaaattcttg atgcaatgga taaagggata aatgttgagc aaatatatca ggcagtaagg
    39961 cttttaaaaa agtatcaaat gaaaccatgt ttatttcttc agtttggtta tcccggagag
    40021 actaaggcag atattctgaa aaccctgaag atggtagatg aacttgatcc gtttgatatt
    40081 ggagtgtctg ttagctatcc acttccgggt actgtattct atgagaaggt taaggcatca
    40141 atgaaagaga aaaaaaactg gaaagattca gacgagttgt tgctgatgta ttccggtgaa
    40201 tatagtgcta cgttttataa agcacttcat cgttatgtac ataaatcttt tcgtgcaaaa
    40261 caaagctggc aagagttaaa aaagattttt accctgaaaa atggaaacat acataaagca
    40321 ttgttgactg catattataa accggctagt attattctga gatcaggcgt aaatctaaag
    40381 gaaaaatagc gtaaaatatg gagacatctg tacaagcatt ttcactacaa gctaaggtgt
    40441 ttgatgatta caatgagtca aactgtcttt cacgttattg ccgtgatgaa atttataaaa
    40501 ttgctttaaa gtatattccc ggcaacagcg atattttaga actaaattgc ggcaccggta
    40561 ttgatgcaat ttatttttca aaaaatcata aagtccttgc aactgatgga gcagaaggaa
    40621 tgattaatgt attgaaccat aaaattaata agcaaaatat tccccagatt gaaacaaagt
    40681 gtatttcttt tgaccagcta gatttgatag gcaatagaaa atttgatttc attttttcaa
    40741 atttcggtgg gttgaattgt acggaaaatt tggccgatgt tgtcaataaa ctaccattat
    40801 ttctcaaaga taatggtcga attatgcttg tgatgatgcc gccaatttgt ttgtgggagc
    40861 atttacatgt attttcattt aacttcaaac ttgcatttag acgatggaag aaaagcggtg
    40921 ctaatgctaa agttgaagga gtaaaattta aaacatatta ttatcatccg ggatttgttt
    40981 ccaaactatt aaaagagaag tttaagatag tttacttgca gggtttatgt aatattatgc
    41041 caccggaata tatgcaccat tatcttaaaa gctctcctat gcttttcaaa atgctatcta
    41101 aagccgatag atttataaat cgtatattcc cttttaagca aacaggcgat tattttgtaa
    41161 ttgtgatgga aaagaagaaa atatgtatag tgtaagtaaa caattttaca ttattttaat
    41221 cttaatagct tcaatcattt tttttattac cggtttttta aggtttgatg gattatatgg
    41281 gcaggatgct tatagctatt tgttgcaatg caaaaattta ttttcagaaa attatcgttt
    41341 gaatttttat cctccggttt atgcattttc cggatggttg ttgcatttta tatttcatga
    41401 ttttgccatt tcgcttcagt tggtgagttt gatttctgta attgttacaa caatattaat
    41461 cagaaagatt attcttatac agagtagaaa tgaacaacat gcttttgttt tcagtattgt
    41521 cttttttctg ctcagtccat ttatactaag atcatcagtt attgtgatga gtgatgcatt
    41581 ggcaatgatg ttctgtactg tatgtctgta ttttcaactt aaacaaaagg agtcatctgt
    41641 tttttttgtc tgtttattct ttttattggc tattctgact cgttatgcag ctgcaccact
    41701 cttactacct gtattgtttt attcattgct tcgtgcattg cgcagaaaac aatttgttta
    41761 tttgctaggt ggaataggaa ttattattgt aggcttcggg ttgaatgcgc tattctggtc
    41821 aaatgcatcg ggaagtgtta atcatcaatg gttacatcta tggaaatttg taaatatttt
    41881 caggagtagt tttaatactc ctgatggtat tcaggattat ttattgccaa acatattata
    41941 tgtttttaaa ttgtttgtat atccggggtt tataccattg tttttattct tgttgttttt
    42001 tattcaaaag aatgaattgt tcaacagaaa aatgatatta ccaacatcat cacttctgtt
    42061 atattttatt ttcctttgcg gtattccatt tcagaatgac agatttcttt tgatagcatt
    42121 tccatttgct tctataataa tgtttccttc attcagtaga ttaatggaac gttttaaacc
    42181 tcctctttgg attgttgttg tggtttgtat tgttcatgta gttttggcat gcagagctat
    42241 attgccgatg tatcatcgaa atattattga aactgaaatt gcaaatgcat tgaagcccta
    42301 tcaaggaaaa gttttgtatt catttgatat ggatgttgct ttaaagggaa gaggattaaa
    42361 ttttgacttt aaaaatttat tagatacccg gtatcaatca tttgaggaaa gtagtctgat
    42421 gttatttaat gaaaagaaaa tgggcaagca atgggctggg agaaatccaa tgattaattt
    42481 taatgaagca aagtcaagtt atttacttac tgtagaaagc gagcttaacg atggatggat
    42541 tttatattta gttcaaaaaa gtaaaacaag gtgaaagttc tttggataac acccggcttt
    42601 gcatcagacg aatctgattc aacatgtatt ccgcctttgc aggacttagc aagagcacta
    42661 caagaacaaa atattgagct gactattatc gctcttgact atccgtttaa tagaaaagaa
    42721 tatcattggc atggcataag agtttatgca ttaggtggtg ctaacaaaaa aggtctttca
    42781 agattgtttt tgttgcagcg cacatggagg ttattaaaaa gtatagaaga aaaccatgag
    42841 gttgatttag ttcattcttt ctggttaaca tggcctgcag ttgtaggtca acgtttttgt
    42901 aaaaagtata aactacctca tcttcaaaca atcatgggtc aggatgccat gaagaacaaa
    42961 ttttatagtt ggtttgattt taaatattca gcaatcgtaa atctttcaca attcgccaat
    43021 gataagtttt atctgtctgc aggtcggcag agtgatgcaa taattccatt tggattagga
    43081 ccgataagtg cagccgttgt tgagcgcgat atagatttaa taaatgttgg ctcagtgaca
    43141 ggattgaaaa atcaattgct ttttttaaga attgtgaatc aaattcagca gctcaaattt
    43201 aatgtcaaag ctgtgttaat tggaaaattt tacgatttgc atttgattag aaaactgaag
    43261 cagtttattg ctgaaaatca tttggagggt attgtggagc ttttggaaga aatgccacgt
    43321 acagaaatgt ttaaatattt aggaagggct aaggtgatga tacatacatc tacttttgaa
    43381 agtcagggca tggtaatgct tgaggcaata gcacatggct gcaaagtata ttcatcaggc
    43441 aagggaatgc atttcaacca tgagttattt tctcttctta cagatgatct gaacgagaat
    43501 gttaattcta ttttctctta tttgttaaag aaaaagaatc ctcatggaga aaaaattgtt
    43561 attattgaac aaacagcaaa tgattatttg gatctctata aaaaattatt aaaaaaataa
    43621 ttattctaac gatcgctttc gtgtagtaat gtagtttgtt ttctgatgat tttaataatt
    43681 acataaaaca caatactgaa gactgttcca taaatcagaa aattgttttg aacagcattg
    43741 ttggtaatta attgtcctgc tgcataaaat agtaagaaga ctataaatac tgcagcaaat
    43801 ccattttttt ctttcttaag taccttcttc cagctaaacg gaagtgatgg ttttttataa
    43861 tttgaaaatt caagaataaa tgccggtgtt ttctctgccc agtcggtgta ggcattgccg
    43921 aacttctttc tcataaattg ctcttcggca tacatgatgc gctcgtagta caaccagtac
    43981 actgcaataa acaacaatac gaaccaaatg ctcgctgtaa acaatgctat gccaagccac
    44041 atgataaaat taccaacata caatggatga cgtaccattg aatacatacc ggtttggtta
    44101 atggtgtctg ccacctgacc ttctgaagta ttacgacctg atgtattgac aggagtatgt
    44161 ccaacaatat aaatgcgcat caacaatcct aaaaacgata ctgccataca ggcatataag
    44221 aaccattctt cagaccttga gttttccgga aatggtctta gatgatgtgc cttgctccat
    44281 aaataataag acaagcctcc tgctaaaaga accaagggta acatactacg gtatttgaac
    44341 aaccatagcc cttgtttttc catctcttcc tgtaaggcca tacattttga atttggcaca
    44401 aaaataaaaa tagaacctta cgacaagatt ctgtttttat tttaatttaa aaattatgtt
    44461 aatagctaag gactactgaa ttagagtttc cattacttgt ccatgatact ttaaagccat
    44521 aagcacaatc gagcgtggtt gcattaccat tttgatcaac gccaagcgta agagcaaagt
    44581 ctgacgcatt tacgtgatga atcaactccc ctgaattggg gcgccataag ccacagtaag
    44641 tatttgaaat tattggcgtg gtatagtttg tggtaaaagc ctgtccatat ctgtttgttc
    44701 cccacatact gctgtttgaa tagttgttta ctgtagaatc accattggct gtaaatatga
    44761 ggtaagcacc atgatttcca gatgggacat acgaccattc tgtaatattt gcctgattcc
    44821 agactgctgt tgaaccatca tcataagcca cgttaacatt gaatgcacgg ctttgatatt
    44881 taaaactact tgttccggcc agaaaagtta accagttgtg cccgttaaca tttgtcagtg
    44941 ttttaacacc gttaaaggtg atagttttat tatcactgag tcgtgttatt ttaaagttgt
    45001 tgtaagtaat ggtaagaact gatcctgcaa gatgccagtt tgtacccgta gtcaattgca
    45061 ctttaatttg tccagaacgt gtgcgcgatg gactaaagca aggtgtaata ccatcaaagt
    45121 taaagaataa gattttctga gaaagctgag atgaatcaat tgttacacca caaagtgggc
    45181 ttgataccac accggcacct ctgccgaatg cagggatttc gttaatggtg ttgttgatgt
    45241 cattatcagc ctgatcggat tctgctttgt aattgttaga atcgtcatta aactgtttcg
    45301 tcttagcatc taatgcttgc tgatcttcct ttttgcagga ataaaagagt gtgataagcg
    45361 ccaatgctga tgtaattaag atgagttgct ttttcatttt tacagaattt agaagtaaaa
    45421 gtaaccattt tatgcatcgg aagtttgttt ttttttagaa acgtcacatt ttaaacaata
    45481 ttgttatttt cacttttgtt tgaaataaac taacaaatca tcaacaggcc ggataatggt
    45541 tttgcccaca aaaaactttt tttctgatgc aatacgttca agaatacttc gaaaatgaaa
    45601 tgcaatactt ccaataaaat gcacagggta attttgatgt tggggataag cgagtatgct
    45661 ggtgtcagca aactcagtaa gacctttgta tatcagatgt tgtatgtaag gatgtgaagc
    45721 ttcataagat aaaaactttg caaatgatgc catataaaca tttgctcccg gcaggtggta
    45781 aacattctta atagcacttt cttttgtaag atgatagtgc tcatagaatt tcttttttaa
    45841 atcatcaggc attatgccat acaggaaatg tgtcatcaac tttctgccaa aatagcttcc
    45901 gctgccttca tcacctaaaa tataacccag cccgtgatta gtaggatgaa ggtgtttgcc
    45961 atcccaaaaa gcaatgtttg accccgttcc taaaatggca actaacccgg ctgtatcaaa
    46021 gcatgcagct aatgcagcac ctaacatatc atgatcaact aaaactgttg cgttgggaaa
    46081 catactttta aaagctgatg ctacaatatt ctttctgtca tcgctggagc aacctgagcc
    46141 aaaaaatttt atatccttta tttcatttgc atatttaaca agcgaaggat tttttttcag
    46201 ttcgtttaaa ataaattcag tgctgtgaaa aaaaggattg aaaccgattg tttcaatttc
    46261 agtcttaaaa attttatggt ctgtcaaaag ccagttggtt tttgtagagc cactgtctgc
    46321 aactaaaagc ataaagttta ttttagaata tgttttataa agataagaaa ttattgtatg
    46381 acaccaatcg tttatcgaaa ggattgtgtt gtttgactac tttttcaaga tctatcaagg
    46441 tgacttcttt cccgttccac cctgtcattt tatttgtttg accattgaga agtgcagcta
    46501 ctgcttcggc accaaatcgt gttgccatta atctgtcttt cgcagttggg ctgccaccac
    46561 gttgaatatg tccaagcact gtaaccttta tgtcgtagtc aaaagatttc tttacagctt
    46621 ctgcaatttt aaatgcaccg ccaccatcat cgccttcggc aacaatcacc acacttgaag
    46681 tttttcttct aagaacactt tcttttagtt tttcaaccag agcattgatg tctgtttttt
    46741 gttcaggcat taagatttct tctgcaccac aggcaattcc tgcataaagt gctaagcagc
    46801 cggaatcacg acccattact tccacaaaaa ataatctgtc atgcgaagct gctgtgtctt
    46861 taattttatc cactgcatca attattgtat tcaatgccgt gtcaaaacct atagtgtagt
    46921 ctgttccata caggtcatta tctattgttc ctggaattcc aatgaatgga atattattga
    46981 atttattaaa tgctactgct cctttaaacg taccatcacc accaatagca ataatacctt
    47041 ctattccttc ttcacaaaga ttgtcataag cctttttcat gccttcagca gtcataaaag
    47101 ccttgctccg tgcagtttta aggatggttc cgcccctttg cagaatattg cttactgagc
    47161 gggcatttaa ttctacaaat tcaccatgaa tcattccttc ataaccacgc atgatgccta
    47221 tcacatttct attttgtgaa atggcacaac ggacaacaga gcgaatacag gcattcattc
    47281 ccggtgaatc acctccggag gtaagtatgg caattttttg aatgttcata aattttgaaa
    47341 aaagacaagg acaaatttaa tccattaact tctctttttt acttttacac cattaaaaca
    47401 ttataaatta aaaaatcaca aatgacaaaa tttacactat tagctgcatt cgcagtattg
    47461 ttatccggtg cttccatagc gcaagaccgg gttcctgtta atctgaatcc tgcattcggg
    47521 gcaagatcac aatctgtaac aaagacagaa tacacaaatc ccggacagtt gatcccaaga
    47581 aatggcaaca gtgccattaa tgttgctttt tctgatgatt tcagtcagca aaatgacacc
    47641 acttcattaa ttgccagagg ttacctgcct tattatagag gaacaggacc acaaggagca
    47701 caggcaacat ggtttcagcc tgacggaact gtatttcctg catataatgg agctgcaact
    47761 gagtatgttg ctgcaaatta tcaggtagtg accggaacta ataacattga cagctggtta
    47821 gtaacccctg ctttaaatgt ttctgcaggc gatattattg cattttatgc aaggtcggtt
    47881 gcaggttcaa catgggtaga ttctattcgt gtaatgtact cagctgcagg agattcaaca
    47941 ccggaaggtt taaattgggt tgaattaggc agatttcagg caagtataac aggtacatgg
    48001 gactatataa actatccggt aactgctgcg ggcacgacag cacgcttcgc aatccgttat
    48061 actgttgcag atggaggtcc aaacggtagt aatagcaact ttattggagt agatcaactg
    48121 gatatttata cccctcaagc cattgatgcc agcgtttttg caataagtaa tatgaattca
    48181 ggctgcggtt taagtgcaac aacaccagtt gatgtttcta tcttcaactg gggtggaagc
    48241 gcaatttccg gatttcctgt atcatttgtt gttgataatg gtacacctgt tacggagact
    48301 tttaccggaa caataccagc aggaggttca tcatcattct tgttcaccgg tacagctgat
    48361 ttttctgcag ttggaccaca tacaataaaa gcttacagca gtcttccttc tgatggtaat
    48421 gcatcaaacg atacaacaat agctactgta acaaacatcc ctcctgtagt actaggttct
    48481 actccagtaa cagaaggttt tgaaatggca tcgggtacta caccgcctcc gggatggact
    48541 attgaagata ccgatggtga tggtaacatt tgggattttg ctactactta tacccacaat
    48601 ggtctggtat gcgcacgtct tggtgttgga aatccattag ctgcaactcc tgaaaattgg
    48661 ctgttttctc catgtttgga tttaactgcc ggaaccgatt atagaattga atattggtat
    48721 aagagcttta cagcaactgc aacttcatat cagttagaat tgcgttatgg aagtgctgcc
    48781 accggtgctg caatgacaaa caatgttgct gttgatccat tgtttactga ttctacgtat
    48841 cacttggctt ctcacacgtt ctcaccaaca agttctggtg tatataacat tggtattaac
    48901 ggctttggtt ccggtgttga caattcaata agaattgatg atgcttcaat ggtagttgta
    48961 acaggtatta aagataatga tttgagcagc aatgtgactc ttatgccaaa tcctactaca
    49021 acagaattgt tggtttctgc taaactttat ggcaactcaa cacttactgt ttataatcaa
    49081 ttaggtcagg aagttattaa gcaaaattat aatgagcctt tccgcattac cttagatatt
    49141 caatcgttga ataaaggtgt ttatacactt aaaataaaca atgccgatgg tgttgttgta
    49201 aataagtttg taaaacaata attaatcttt tttataagaa accaccggct gtcagtcggt
    49261 ggtttttgta tttttataca agtatgaaaa catcagattt ctttaatact gacgaaattt
    49321 ctttttttga aaaggtatat caggttgttc gtttaattcc caaagggagg gtaacaagtt
    49381 atggtgctat agctaagtat cttggcagtg ctcaatcatc acgcatggta ggttgggcca
    49441 tgaacaacag tagcaggata aagccaaaag ttcctgctca cagagttgta aatcgtaatg
    49501 gcatgctcac aggaaaacat ttttttggtg ccaatgatgc catgcaatat ttactcgaaa
    49561 aagaagggat taaagtatct gacgataaaa taattgattt taaaaaacac ttttgggatc
    49621 catctgttga attgattcta taaacactca tttacaacca ttatctttgc accatgcaga
    49681 ttacaaaaga aaaagtatta gaagctttaa gttatgtaca ggaaccggat cttaaaaagg
    49741 atttggtgac actgaacatg ataaaagata ttgaaattga agggcagcat gtgagtttta
    49801 ctgtaatcct gacaacacct gcatgtcctc tgaaagctga aattgaacgt gcctgtaaaa
    49861 atgccatagt tcattttgta gataaagatg ctgttgtaac agtgaatatg actgccaacg
    49921 ttactacaca gcgcagtatg gataatgaaa tgatgaaagg tgttaaaaat attattgccg
    49981 ttgcttcagg caaaggtggt gttggtaaat ctactgttgc tgcgaattta gcggtttcat
    50041 tggctaagaa cggtgctaaa gtagctttgg ttgatgctga tatatttggg cccagcgtac
    50101 ccataatgtt tggttcagag catgagcatc cttttattga agaggttaac ggaagacaat
    50161 taatgatacc tgtagaaaag catggtgtga aacttatttc aataggctac cttgccgatc
    50221 catctcaggc aattgtttgg cgtggaccta tggcatcaaa agccttgaaa caacttttta
    50281 gtgacgttaa ctggggagat atagattata tgattgttga ccttcctccg ggaaccagcg
    50341 atattcatct tactttaatc tctgcagtac cacttacagg tgctgtagtt gttactacac
    50401 cacaagatgt agcattggcc gatgccagaa aagcaatagc aatgtttcaa cttccatcag
    50461 taaatattcc tgttttagga ctaatagaaa atatgtcatg gttcactcct gaagaattac
    50521 ctgataacaa gtattatctt ttcggaaaag atggtggaca aaagttagct gctgaaatga
    50581 atgttaagtt gcttggtcag gttccattag ttcaatctgt tcgcgaagga ggtgatatgg
    50641 gaaatccaat tgcaacagga agttcaatcg ttgcagcagc atttaatgat ttatcacaat
    50701 ccgttgcaca gcgagtagca attcgtaatg ctgttattga accggcacaa cgtgttcctt
    50761 cagcaggata ttaaaaagtg taatgatgag tgagtctgta aaaaataatt taaagctaag
    50821 ggtagaacag gcattggata ccatcagacc ctatctgaag tctgataatg gtgatgttac
    50881 cgttcttgat attacaccac agaatgtagt aaagttaaga tttgaaggag catgcagttc
    50941 atgtacaatg agtgcaatga catttaaggc aggagttgaa gaaacgattt tgcgtatggt
    51001 gcctgaaatt actgctgtag aagcattgaa tgtggagaat gtttaaataa tgatttttta
    51061 tttctaaaat aaagtagtct tgttgatgtt atcttctaat gaaagattcc ttccgatgaa
    51121 aataatagct gttatttttt acttattgtt ttttaccggc attattcatg cacaaacctt
    51181 tgttgttttt aaaggagata ccataaatcg taaagatact aaaggactta aacagggtgt
    51241 atggaaaaag ttttacagaa ccgacagcct gttttcagta acaacgttta aagacaataa
    51301 acaaacaggt actacaaaat tcttttacga atcaggtaag ataaaatcaa cgctcatctg
    51361 gaaaaagccc ggacaatttg cttttgcaga aagctttttt gaggaaggga agaaaatgtc
    51421 tgatggttat tatctgaatg agcagaaaga cagcacgtgg cactattata atgaagaagg
    51481 aaaaatcaca tcacaggagt cttatcgcaa aggcataaaa caggggaaat ggcttacctt
    51541 ttatcccgaa ggtggaaagg tggaggagaa aaattatgta aatggaaagt tggaaggacc
    51601 ctacttaaag ttttttaaag acggaaaact aatgttaaaa gcaacctata agaacgataa
    51661 gcttactggt ggctatacca tctacaatgc tgatggtgga tattatacca cagggcaata
    51721 tagtgccggt gacagggctg gcaagtggac tgtttttaat gagaaaggca agccttggat
    51781 tgagagccaa tataaaaatg gatttgaagt taagaccgat acgcttcagt aataattaaa
    51841 gtaaatttgc aaggtgagta agaaaggtag aattctggtt gccatgagtg gtggcattga
    51901 tagttctgta acatctatga tgctgcatga cgagggttat gaagttattg gtatcacaat
    51961 gaaaacatgg gattatgcat catcagggac ctccggtaaa gaaacaggtt gttgcagcct
    52021 cgactctatt aacgatgcca gaagtattgc tgtagaaaaa ggatttccgc attatatact
    52081 tgatatcaga ggtgagtttg gcgattttgt aattgataat tttgttgatg aatatcttgc
    52141 cggacgaaca cccaatccct gtgtactctg caatacgcac atcaaatggg aagcattaat
    52201 taagcgtgcc gatatgttgg actgtgagtt tattgcgacc ggacattatg catctgttcg
    52261 tagtgaagga ggtcgttatg ttgtaagcag aggaaaagat ttaagtaaag atcagagtta
    52321 tgtgttgtgg ggtctaagtc agaaaaatct ggcaagaaca cgatttccat tgggcaatta
    52381 taaaaaatca gaaattcgca gcatggcttt cgaaatggga tttaaagaat tagccaataa
    52441 gagcgagagt tatgaaattt gttttgttcc ggataatgac tacagggcgt ttttaaaaac
    52501 aagggttgaa ggcattgaag aaaaacttga tggtggggta tttgtgaata caaaaaatga
    52561 agtaatcgga aagcataaag gatatccttt ctataccatt ggtcaacgta aaggacttga
    52621 agttgctttt ggcaagccaa tgtatgttac agaaataaaa ccaaacacaa atactgttgt
    52681 tttgggcgag ttggatgatt tacagaaaac tgctatgaaa gtgcgcggct taaatcttgt
    52741 aaagtatgaa tcgctcagta aaccaacaga agtactcacg aagataagat ataaagatcc
    52801 cggcacaatg agtgttataa cgcaaacatc ggcaaacagt gctgatgttg aatttcatgc
    52861 accggttact gctgttgcac ccggacagag tgcagttttc tacgaaggca acgatgtagt
    52921 aggaggtgga tttattgata aagaatatga caaagtctaa aaatattttc atactactta
    52981 acctagtttt ggtattgatg atgggatgta ctaaagacaa agaaactgtt agtgctattg
    53041 actatcgtta ttacccaata gcagttggtg attctatgat ctatcaggtt agtttattta
    53101 ataaagacct taatacctat gacagtacct atcagttatt agaaagagtt gagtctgtat
    53161 tcaatgataa cgaaggtagg ccaactttcc gcttagaacg ttacgtgcgt aagtctgatg
    53221 cagatgcatg gagtatttat aaagtttgga cttcaaataa gacttcaacc agtattgaga
    53281 aacaagaaga caacattacc tatataaaac taatttttcc ggtgaaactc aataaaacat
    53341 ggaatggtaa tgcaaagaat atttttgctg atgactttga agattataaa atcatttcgc
    53401 tgaatgaacc attgcaatca ggaaattatt catttgacag tactttaaat gtacgccaaa
    53461 tagattatga ttatggcttt gagaaaagaa aacattttga aatttatgct tccggtgtag
    53521 gtatgattta caaagatgag tatgattctt tgcaaggaag tccgcttgga aaaatatatc
    53581 attacacaga gacattactt tatcacaata aaaaatagac atgaaaaaga tttacttatt
    53641 gattctgatt ttcattacat cacaagagtt gtatagtcag aatcgttatg cagtaatgct
    53701 gacggataaa aacaactcga cttatagttt ttctaatcca tctgcctatt tatcgcaaag
    53761 ggcaattaac agaagattgc agtttggaat tactcttgat tcactcgatt tgcctgttaa
    53821 ttcaacatat cttgcagcaa ttcaaaatac cggagctgtt attttaaata catcacgctg
    53881 gcttaatgaa gtgacagttg atgtgtctgc caatccgggt gcactttcag caataaatgc
    53941 attgcctttt gtgaaacaaa caaaattggc tgcacgaaca acaaacagat caaacagcaa
    54001 gtacagcttt gagatggaaa gtttaatgca gcgacaatct cagacacaga aagtggcttc
    54061 cacttcgtca ttttataatt atggcaatgc actcaatcaa attcaaatgt tgcatggcga
    54121 caacttgcat gatttaggat tcagaggcga tgggaaaatt attgctatgc tggatgccgg
    54181 ttttctaagg gctgatagta tgactgcatt tgacagtttg cgtgcacata acagaattct
    54241 ttctacttat gattttgttg atcataatag taatgtttat gatgaccaca cacatgggtc
    54301 aatgtgtttt tcaatcattg gtgctaatga tcccggaaat attgtcggta ctgcacctga
    54361 agcttcattt ctattgtatc gttctgagga tgcggcaaca gaaaaactga ttgaagagta
    54421 caactgggca acagctgcag aaacggcaga cagtgcagga gcagatatta tctcaagttc
    54481 attaggatat agtgtgtttg aggattctac aatgaatcat acgtacaatg atatggatgg
    54541 ccgaactgca cctatatcca ttgctgccaa cattgcttca cgaaaaggga ttgttgtagt
    54601 tagcagtgca ggtaatgaag gtaatggtca atggcattac ataacagcgc ctgcagatgc
    54661 cgatagtata ttaaccattg gtgctgttga tgcagcagga gtttatgctt ccttcagtgg
    54721 gacaggtcca tcctttgatg gaagaataaa accaactgta gtggcacaag gacaaggtac
    54781 atttgtttca gacccttatt ccaatgttgt tttctcgggt aatggaactt ctttttcatg
    54841 ccctgtaatt gccggattgg ttgcatcact atggcaggca attccgtcag caaacaatat
    54901 gcaaataata aatgctatag tgcaaagcgc atcacaatat agcaatcccg attcattgct
    54961 gggttatggt attccaaatt ttgattctgc acggaattca ctacttgcag tatttaatcc
    55021 atattatggc aaaggtgatt ttatagaaaa aatattccca aacccgttta agaaccaact
    55081 gaacattaat ttttatgcag acagtaacag ccaatatact tcagagatat ttgacctcag
    55141 tggaaaacga gtaatggtta aacacggaga atttgttcca tttacaatca acaacataaa
    55201 ccttgctact gccaatatgg gaaagggtgt ttatttattg cgtatcacta ccgataaaaa
    55261 tgtgtttcag gcaagaattt tgaaacagta agtttcaatg tcatcaatag ggaagaagct
    55321 taaaatttta ggaaccaatt ttgcttttct aatattgttt ttatccattg ctgaagtcgg
    55381 attgcgcatt gcaaatatcg gctatgacaa tgctgctttc gagcccgatt ctgtactgca
    55441 ccatattcac agacctgact attcttttat tcactcaaat gctgcagaac atgagtatgg
    55501 taatattgct gtaagatatg ataagatggg ttgtgttgtt aacccacatc aaaagaaaac
    55561 agagcattgt agagaaaaaa tttggttttt tggcgactca tatatcgaag cacttcaagt
    55621 aaagtatgat tcatctgtta caggattgtt agaaatggaa aatcctgacg tgtgttttaa
    55681 aaattttgct gttggtgcat atagccctat tctgcattat ttaaaattaa aacagttatt
    55741 gaaaagcgaa aagcctgata ttgtcttttt gtttttatat gccaatgatc cttcagaaga
    55801 caaacaatat cttaaagaag caaaaattta tcatggagaa gtagcagctg ttgatggcgg
    55861 tatgccacag tggaaatatg aaatcattag aaactcaaac ctgcttcgtt tgattcgaaa
    55921 attttatatg cagatgactg tgaaactgga aaataagcag cgtaaagaag agtatgtttt
    55981 gagtgaaaat aatgtaatac ttgctgaacc tacactaaca tatttagaaa aatttgtaat
    56041 gctttgtctg aataaaaatg taaaagtagt tgtgtcgtgc ataccatcaa aaacagatgt
    56101 gctgagaagt gattttaatt ctgccagttt ttcaaatcaa gtaaaggcat ggtgttccgg
    56161 aaaatgtgat tttcttgatc tgcaggagcc gttacagaag ttctatttgg caactaacac
    56221 ttcgccatat ttcaaaaatg atattcattt taataactat ggccaccgca tagttgcaag
    56281 gacaattgtt ttagctcaaa ttatcaagga ctgatttgat tttattaaag tcaggaattt
    56341 cacctgcatt ttctaaaact tcaatgtagc gtagtattcc gtttttatca atgagaaatg
    56401 ctgcacgttt tgtaataccc ttcatgccta atccaaacaa ctcatactgt gcatggtaaa
    56461 tttttcctat ttccttattg aaatcagaga ctaattgaaa gtttaatttt tgctcttctt
    56521 tatattttgc tagcgaataa attgtatcgg ttgagatgcc aattactgta cagtttaagt
    56581 tattataata agacaggtta tctctgacac tacacaattc ttttgtgcaa gtactggtaa
    56641 atgcagccgg aaaaaataaa agtaaaacat tcttgccttt tagttgctca agtgaaaagg
    56701 gttgcttttc tgaattgaaa ctttcaaaaa gcggagcagg atgattgata tttagcgaca
    56761 tcgtatttaa ttatttagat ttttatttct gtttgaatta acatttgtat ggttcattaa
    56821 atggacaagt gtttttaggt tttcaccttc gcaatagcag cgtaaatggt gacatttatc
    56881 aatcaagtca gtcatattaa ttagagttat tttagagtta ggaaaatgtt gaataaaata
    56941 atcttttagt ttaattgctt cattgttagc gttacatccc aaagtagtgt tgccatgttt
    57001 tcttggcccc ggaccaaaca attctaagaa atagtctttc ttacgcagta caaaatcttt
    57061 tttattgtac aacacaaata aattatagtt gtcactcttt ttatatggaa atgtactttt
    57121 tctggaatag tagcttttaa aggcttcaat tccacttatt gccggtgcaa taagacaaat
    57181 attaatattt ctgttattgg gtgtaggata gttgtttact gttgtatcaa acaaagcatt
    57241 ggttacaact gcggcaccaa ggctgtgtga tataatattg acttccggtg aatgggttcg
    57301 tgaaagtatt tgtcttaatc catagccaac tgaaatagca ttaagttgta ctttttcaaa
    57361 aaccgtaaaa atttgtttca tctgctttac accttctgat atatccaagt agctaccatc
    57421 ccaaaatacc cttatgtagg aatgcttttc agtacttaga cgatttattg cctccattaa
    57481 taaaccataa tctattacag aggtaaaaac acctggttta ttaacatatg attttctgaa
    57541 accatgaatc ataaaagtcg gtaccatgac ttcttttgtt cgattgttta ttttatcggc
    57601 attggtttga aaaatcgaat catttagaat acttatgttc gaaatactat aatcactaaa
    57661 acgacattta tataaagagc atatcgctgc aaattcattt ggattattta agtaaaaagt
    57721 atctaaagat ccggcactga tattcaatgc agagtcagca ataaaaaaat ctggataagg
    57781 ctttccgttt ctgtcaaaat agattgataa aattgtgtca ttcaggtttc tttctccata
    57841 caagacaaaa tgttgtgccg tactttgaaa ccaggaaaac agaaataaaa ttattaat
//
LOCUS       DAOQNP010000007        51858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_185_length_51858_cov_8.450572, whole genome shotgun sequence.
ACCESSION   DAOQNP010000007 DAOQNP010000000
VERSION     DAOQNP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 51858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51858
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185_length_51858_cov_8.450572"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKY09_01925"
     CDS             <1..558
                     /locus_tag="PKY09_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNB11640.1"
                     /translation="LAIDATNIMRKHSEFFNADLVNYLHVDEDPFGFSNLKYVRKVEE
                     SKKINDLKGPCIIISASGMIEAGRIKHHIKNNINDSRNTILIVGYCTPESIGGHLMRG
                     DKIIKIFGNEYPVKANVEVISSFSAHADYLELIKYLSCQDTAAVKKLFLVHGEYEVQQ
                     EFKEKLKEVGFKNVEIPEELQSFKL"
     gene            complement(548..1132)
                     /gene="rdgB"
                     /locus_tag="PKY09_01930"
     CDS             complement(548..1132)
                     /gene="rdgB"
                     /locus_tag="PKY09_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNB11641.1"
                     /translation="MTELIFCSNNKHKLQEIRKMLPSGYILQSLSEAGIKSSADETGV
                     TFYENAFLKADAVYLHTKKNCFADDSGLCVDALGDAPGVYSARYAGHEATDADNRNKL
                     LADLEGIKQRNAYFIAVICLIWEGKAYYFEGRIDGNITNVEHGKSGFGYDPVFIPNGY
                     EVTFAEMTEDEKNKISHRQIAVGKMITFLRNLKV"
     gene            1184..5203
                     /locus_tag="PKY09_01935"
     CDS             1184..5203
                     /locus_tag="PKY09_01935"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB11642.1"
                     /translation="MQKIFFALSLLFSLSRNAQAQCPNASFSLPDTVCSGTMLPLINT
                     STGNGLTYQWDFCPREIHGLTSYRYFRYNNYVLNTMTDMDLLKVGNGYYLITMDSISN
                     NFVVGNIGNHLSNQTFDAIINSGFTKLNCFDFIVEGDTNVYGLAAYAPTSTLYLMAFP
                     GGIQVAPVLTPLPTFSTVANPADIKIVHADNIYYAFVASKGNGNVTCLTFGNSLINSP
                     TELYSLNIPGSIKLSDIDIVTTCSPPVGFVSDSKNGDLTKLSFTNGFSNAPTLSVYSG
                     TGSIGNNSVVITQEFESYFVCYTDTANKQLKWLQFDDNNFANTPTVYTSPAFYFGPNQ
                     AESLTDSSITRFIVNDAPTLNFVEMLYFDSCYTKYSEDQNPSFSYLNTGWNKLSLTVS
                     DSNGYQSIAYDSVFVITGPKSQFSYSGNTCFNPNDSITFNDQSTSPSGAITDWLWDFG
                     DGATSTQQNPQHAFTSPGTYQVKLTITAGCEKDTIIPITIHALPIVNFTYSLSCANSA
                     TTLTDQSFSAAGLQSWSWNFGDGSSNNQLQNPDHIFQSGGNYNVQLTVTDSSGCIDSI
                     VNIVQVKATPSTAFMALQTCLGDTVQLSNTSTISDSTALTYFWDFGSGITSTQTDTVI
                     YFTSAGSYPATLIAYSSQGCSDTLNQNIVVSTPPSVNFGFPSSNCQYNAITFSDSTVG
                     FNLSSWQWDFGDGDTSSLQSPTHVYNLSGNYTVQLTVSSGNDCAASYSQTINILEGPS
                     AAFLTGNGCQGVSTNFTDQSTVSSGSSIVAWQWHYGDGDSSQIQNPSHIYAAAGSYNV
                     LLRVTSDNGCQDTTSQPVQVFENPIAGFYVQPLRCANTEIYFLDTSSIGNGNISQWQW
                     QFSNGTTSNLPNPFAIFNNAGSASATLTVITSDGCSNSNSFNFTVRPQPDFTIAHNGV
                     CKGKQTSFNYFPNGNISAYAWTWYFGDNTYAFTPNATHLYATSGTYPFSMAVTDSFGC
                     QKTIFDTLTIYPTPDAAFITNGICQNAPVDFINQTDSTNTQINGWQWSFGDGNSSSQH
                     SSQHIYNGTGNYTVSLIAYAAGGCNDTVFSVVNIKPVPEIAFSMLPPTGAPGNNVQVF
                     NNTTGATSYSWDFGDGSPLNLNENPTHVYNDTGSYVITLTASSIFNCMAQLQKPYDVI
                     VPYVDLWLKSLSYTINNNYLSLSALLSNIGNTTINNFDLLIQPEGKGSFIENGNETIP
                     FATEKNYALHTSISINPSDLPEFICVRIKNVNNGNDLNPDNNEKCISLMPENGSMSVS
                     PNPIEDVLSIHLNLPASSEITLVLYDATGKKLTTLYQGTTDAGFKNFSFKLPYLSKGV
                     YIIGATSESLNKILKFIRQ"
     gene            5208..6338
                     /gene="rffA"
                     /locus_tag="PKY09_01940"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             5208..6338
                     /gene="rffA"
                     /locus_tag="PKY09_01940"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNB11643.1"
                     /translation="MIPFNKPHLTGKETDYIKEVFASGKISGDGNFTKRCHQFLQQKY
                     NFKKVLLTTSCTDALEACAILLDIKEGDEVIVPSYTFVSTVNAFVLRGAKIIFADSNP
                     DTANIDVSKIESLITSRTKAIVPVHYAGVACDMDSINAIAAKHNLYVVEDSAQAIDSF
                     YKGKPLGSLGHLSAFSFHDTKNIISGEGGMLVINDEHFEKRAEIIREKGTNRSAFFRG
                     EVDKYGWVDVGSSFLPSEVTAAMLLAQLESIDKIQTRRKEIWNRYYSLLKSLEEKEFL
                     KLPVIPDYATNNAHMFFIVLNNLETRSKLIDHLKRNNISAVFHYISLHASAYYVSKHD
                     GRALPVSDMLTNQLLRLPMFYDLKNDEVDFICQKITDFFTTL"
     gene            6335..6862
                     /locus_tag="PKY09_01945"
     CDS             6335..6862
                     /locus_tag="PKY09_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNB11644.1"
                     /translation="MKRIALLSDTHGFIDQTIVKYLQDADEIWHAGDFGTLAVSDFLA
                     SLKPLKGVYGNVDGHDVRKVHPAHQKFQLEGIKVWMTHIGGYPGNYSPDIKKTIYTEK
                     PMLFICGHSHILKVMPDPKIPGMLHINPGAAGVHGFHHIRTMVKFIIDKTKISELDVI
                     ELGKRASIENKNILP"
     gene            complement(6854..7324)
                     /locus_tag="PKY09_01950"
     CDS             complement(6854..7324)
                     /locus_tag="PKY09_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HNB11645.1"
                     /translation="MIQRIQSVYLFLVVASMASLFFLPFSSVETTENSITSMHVFEVM
                     GHKTFQDNVQTQNAPFYPILILTVVVIVLSLLNLFLYKHRLKQIRLCQLIMLLIVVLV
                     VLIFQQSETRATDVTHVVYHVGTYITVISIVWVFLASKAIKKDEDLVRSADRLR"
     gene            7534..9273
                     /gene="rho"
                     /locus_tag="PKY09_01955"
     CDS             7534..9273
                     /gene="rho"
                     /locus_tag="PKY09_01955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNB11646.1"
                     /translation="MYDILELSDKLVAELRDIAKELNVENYEGLKKQDLIYKILDHQA
                     IHPELAKSKKGDKPKEEKPRHEKPRHEKKEHKPAAAPAVTEKPVEESATTNATTEVIS
                     EAKAQDDADKPRRKRIIPLGAPQKNESHQQVAAKTPEATPAPQETATAPATAKTNVEE
                     RPQQNQQPQQNQQPQQNQQNDFQQRNRERRFPDNQQQNNFRQQNDYFEFDNAITAEGV
                     LEIMPEGYGFLRSSDYDYLSSPDDVYVGQSQIKAFALKTGDTVKCKVRPPREGEKFFP
                     LTKVELINGLEPTIVRDRIPFDYLTPLFPFEKLNLSGENSTMSTRIMDMFSPIGKGQR
                     GLIVAQPKTGKTVLLKEVANAIAANHPEVYLIVLLIDERPEEVTDMARSVKAEVISST
                     FDEPADKHVKIANLVLEKAKRLVECGHDVVIVLDSITRLARAYNTTAPASGKILSGGV
                     DSNALHKPKRFFGAARKIEKGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMELQLDR
                     KLSNKRVYPAIDLVASSTRREDLLLDRKMLERMWILRNHLADMNPMEAMEFLLDRLRG
                     TKSNEEFLISMNS"
     gene            complement(9412..10332)
                     /locus_tag="PKY09_01960"
     CDS             complement(9412..10332)
                     /locus_tag="PKY09_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HNB11647.1"
                     /translation="MENNNHLLCNPDSGICEIPAKGQTVTEGNKIKSEKKPIKVIYYT
                     DPICSSCWGIEPQLRKLKLEYGNIIEIEYRMGGLLPDWSYNSGGISKPSDVAHHWDEV
                     SVYYDMPIDGDVWLEDPLQSSYPPSIAVKAAQMQDHEKAILFLREIREMVFLKKRNIT
                     KWEHLEAAAKSVGLDLNKFREDYNGRAKELFNEDLTLGKQLGVRGFPTMFFTDTSGKT
                     ETVYGSKPYEAFENVIIKLLPKVQKINYDKSTTAVFSHYQSLTAKEYSVLTETAKSQG
                     EQVLNDLVANGKLEKLTTKNGSVWKLKNAE"
     gene            complement(10434..10919)
                     /locus_tag="PKY09_01965"
     CDS             complement(10434..10919)
                     /locus_tag="PKY09_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11648.1"
                     /translation="MTQKKFLVFVVEDDRHLQKMIVDYLTEHFPDIEINLYKTGEDAL
                     IDLKIGPDLIILDYFLSLYDKDAMDGLTVLSHIRQANKTVRVVMFTGQEDPDVAASAI
                     KMGAFDYIVKSPESFNKLSDIITQLKKQIVNETPPFDKRLLIVLLIGIIMLSALFIFR
                     H"
     gene            complement(10925..11566)
                     /gene="folE"
                     /locus_tag="PKY09_01970"
     CDS             complement(10925..11566)
                     /gene="folE"
                     /locus_tag="PKY09_01970"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNB11649.1"
                     /translation="MNKDPHTIMDDDDDVDGYRKIDHYNHDSLNKLSQSYLEVLQNLG
                     ENTSREGILKTPERVAKAMQFLTQGYHLNAKEILESARFREDYKQMVIVKDIEVYSLC
                     EHHMLPFFGKAHVAYIPNNYIVGLSKIPRVVDVFARRLQVQERLTVEIRDAIQETLSP
                     LGVAVVIEARHLCMQMRGIQKQNSITTTSAFTGEFENDRTRSEFLRLIADKHI"
     gene            complement(11563..11982)
                     /locus_tag="PKY09_01975"
     CDS             complement(11563..11982)
                     /locus_tag="PKY09_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB11650.1"
                     /translation="MIYITRKERFNAAHKLYKPEWSEEKNREVFGKCSNPNWHGHNYE
                     LFITVKGMPNPETGFVIDLKVLSQIVKENVIDPLDHKNINLDVKFMQGIMASTEMLAM
                     QIWNQLHDAIRSQGAMLHAVKLYETENNFVEYFGNEK"
     gene            12054..12719
                     /gene="mqnB"
                     /locus_tag="PKY09_01980"
     CDS             12054..12719
                     /gene="mqnB"
                     /locus_tag="PKY09_01980"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNB11651.1"
                     /translation="MNILIIAATKQEISLFQTEKSEEIHFYNSQEVETLISGPGILAT
                     AFSLTKKLKEKNYDLIINVGIAGSLDENIIPGDVVQVISEQIADSGSEDHDNFLDLFD
                     LGLLNADEFPFKEKWLSPKSFTTPSLEKIKKVKAITVNKAHGNENSIKQLKLRNTGQI
                     ESLEGAACFYVCMLLKTNIIQLRAISNIVEPRNRSNWNIELAITNLNASLNQLLNELL
                     PPA"
     gene            12697..13368
                     /locus_tag="PKY09_01985"
     CDS             12697..13368
                     /locus_tag="PKY09_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11652.1"
                     /translation="MNYFRQHKEIQNPIRIPVAVRKMLDAGESEFLDFKKEITSVHKI
                     AKTISSFANRNGGKLLIGVNDDRTICGITTEEEKFMIVTAATNYCNPTVAVKFKEWQC
                     SDKIILEVSVPESEQKPVFAKDENEKWWVYLRINDQTLLTSKVMVDVLRKKTDSRSTL
                     IEYSEKEKALMEHLVVHKRITLKQYCKLINISRWRATRIIVNLISVGVLQANHSEKEE
                     FYTLA"
     gene            13368..14072
                     /locus_tag="PKY09_01990"
     CDS             13368..14072
                     /locus_tag="PKY09_01990"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNB11653.1"
                     /translation="MELFYSTNIDDNTILLDEIESTHCLRVLRHKIGDSIEVTDGIGH
                     RYSGIISAASKICKVEITEKHFEQPSNKQLHIAIAPPKSIDRFEWFLEKATELGIETI
                     TPLQCRYSERKHINAERCGKIILAAMKQSNRVYLPVLNEMISLEKFITIPSTAKKLIA
                     HCEESEKLTLEKSIAAEQVIVLIGPEGDFAPHEIKLAIDAGYENISLGQKRLRTETAA
                     LHVCSAFALKNRTGQS"
     gene            complement(14102..14518)
                     /locus_tag="PKY09_01995"
     CDS             complement(14102..14518)
                     /locus_tag="PKY09_01995"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB11654.1"
                     /translation="MAIKRNATAFWNGSGKEGKGTVSTQSTVLNQSQYSYNTRFEDGV
                     GTNPEELIAAAHAGCFSMKLSFVLGAAGFTPDAIETKCEITLDNGAITNSHLILNAKV
                     AGITKEKFEECANEAKANCPISKLLNTNITLSATLA"
     gene            complement(14555..16051)
                     /gene="hutH"
                     /locus_tag="PKY09_02000"
     CDS             complement(14555..16051)
                     /gene="hutH"
                     /locus_tag="PKY09_02000"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNB11655.1"
                     /translation="MKHQISSKHLDFNTLKQIIDSKVAIELSEDAVKRITHCRSYLDD
                     KVNSLAEPVYGINTGFGSLYNKSIDTADLGKLQENLVMSHACGCGEEVPAAIVKLMLF
                     LKIQGLSYGNSGVQLQTVQRLIDFYNHEIFPVVYTQGSLGASGDLAPLAHLCLPLIGM
                     GEVYFKAKKVKSAEVLNQFGWQPIKLQSKEGLALLNGTQFMSAYGTYAVMQSQRMMLL
                     ADIIGALSLDAYDGRIEPFHHLIHQIRPHKGQIATALRYRQLLTDSEMIKRKKAHVQD
                     PYSFRCIPQIHGASKDALDHVSFVIFNEINSVTDNPTVFPDDDLVISAGNFHGQPLAL
                     VLDFLAIALSELANISERRTYQLISGQRGLPSFLVADPGLNSGMMIPQYTAASIVSQN
                     KQLCMPASVDSIVSSNGQEDHVSMGANAATKLYKVLGNTEKVLAIELLNAAQAFEFRR
                     PLKSSAPLESFLSLFRKEVSFMNTDRILYTDIQSSINFLQSVNVNQYE"
     gene            complement(16086..17450)
                     /gene="hisS"
                     /locus_tag="PKY09_02005"
     CDS             complement(16086..17450)
                     /gene="hisS"
                     /locus_tag="PKY09_02005"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNB11656.1"
                     /translation="MKPSIPKGTRDFSPSEMVRRNFIFNTIRKVYERYGFVQIETPAM
                     ENLSTLEGKYGEEGDKLLFRILNSGEVVQDAKSKGDSLKISDLSEKGLRYDLTVPFAR
                     YVVMHQNEIAFPFKRFQMQPVWRADRPQKGRYREFYQCDADVIGSTSLISEAEFLLII
                     QEVFNSLQIGVTIKINNRKILAGIAEVIGHADKIIDITVAIDKLDKIGEQKVKEELLT
                     KGLSADAINKMQPLFESHGDFESKLNFLKTFLAEVSIGLKGIEETEAVIRYLNAFGKQ
                     LQNLELDITLARGLNYYTGSIFEVKVNDSRSTFSGSVLGGGRYDDLTGIFGLPNVSGV
                     GISFGIDRIYDVMLEMNLFDQLKLSETTSQVLLVNFGQEEEQFSLKILNELRQAGINA
                     EIFPEHAAKMKKQMSYADSKKIPFVVLIGKDEMQSGSLTIKDMATGQQEKLTLNDIIS
                     KLSK"
     gene            complement(17618..19306)
                     /gene="ettA"
                     /locus_tag="PKY09_02010"
     CDS             complement(17618..19306)
                     /gene="ettA"
                     /locus_tag="PKY09_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNB11657.1"
                     /translation="MSDDKKIIFSMVGVSKTVPPQRQILKDIYLSFYYGAKIGILGLN
                     GAGKSTLMKIIAGIDKSFTGEVVFSPGYTVGYLEQEPKLDESKTAKEIVMEGVQPIVD
                     LLKEYDDVNNKMGEEEYYSDADKMDKLLNRQAALQEKIDATDAWNLDNKIEVAMDALR
                     CPEADAPVNVLSGGERRRLALCRLLLQQPDVLLLDEPTNHLDAESVDWLEQHLKQYAG
                     TVIAVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSNRLAQEEKQESKRQKT
                     LQRELEWVRMSPKARQSKGKARLNAYENLLSEDVKQKEEKLEIFIPNGPRLGNEVIEA
                     IKVSKAYGNKVLYDDLNFKLPPNGIVGIIGPNGAGKTTIFRMIMGLEKPDSGEFKIGS
                     TVRIGYADQTHKEIDPEKTVWEVISGGNESIELGGKLLNSRAYVARFNFSGADQGKKC
                     GVLSGGERNRLHLALTLRSEANVLLLDEPTNDIDVNTLRALEEAIENFAGCAVIISHD
                     RWFLDRVCTHILAFEDDSQVYYFEGGYSDYEENKKKRLGDVTPHRVRYKSLVKN"
     gene            19571..20431
                     /locus_tag="PKY09_02015"
     CDS             19571..20431
                     /locus_tag="PKY09_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11658.1"
                     /translation="MKHFTTFLILLLTTIVKLHGQVIYNYAGDLNGQPMYVDANVSGT
                     PLYTAGILGYRCDSGYTVRTNGNDPFFHFQKGPVDEFDFDANDRIDFTLTPQSGYQMN
                     ITGMTVVAGRLTYGPEHLKFGLNINGTGLIEGVQMDTLPLECDGDPLNGIPNAVPYTQ
                     VWDITDFNSLSPVTFTIGCFGGINNVAIIYQIIIHGTVTPAQTIPQISSYSHPGGYMY
                     CDDESITLSVVASGTPSPSYQCQDQYGNAILGATQSTLTLPQPLPQHSYYRVKVYNTF
                     GETYSAYSHI"
     gene            20564..22798
                     /locus_tag="PKY09_02020"
     CDS             20564..22798
                     /locus_tag="PKY09_02020"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11659.1"
                     /translation="MLSTSYNPGPGDLLTGNVQLNLEGYVLNNFGYEVCWRQDYLNLT
                     IGIPPTANAGNDQVILQSNTTLTANDPSPGNGLWTVISGTATFTDATLYNTNVSNLSN
                     GNNLLRWTITMGTCSSYDEMTVTVATKPVSAFLSGDTTICNGGLTQLKIDFTGSGPFD
                     YSYTDGVSVFGPFTAVANPEIIWVNPASTTNYQLVSVSSGGTSGTVSGSANVQIFTAP
                     PSSNVTLPFNGMPTHICNGTTATLSIASVAGATYYIWDAPAGSYFDGNPLNTSPYSST
                     LPSVQITFAAPSGSFYTIGVQAANPCGTTLRKIEKVRGLISTPTAINGLTIACANTNG
                     SYSINAVTGATSYQWSISGDATVSGNGTSVSVSFGPTWTSGTLCVAAQTGCYTTVSKC
                     INIENIVTKVYTPTGTFAACPNSVLTYSIPSQPGVTTYNWALPNGANGYSTTNSIIAT
                     FGPNFINSGNICVSATTICGTTTPPKCKTIKIGLPERPTSITGSTTGLCNTSQLYTCP
                     IISGATYSWTAPAGTVITGNGNNAVTIDFGTFTTGSLCVSATNSCGTGSPRCITVKGA
                     PAKPGVISSLPNTWCAPEAGVQFNSNISNLTGNYNLKWQIAPSTNATIQGPNNTNQMS
                     ADWLVSGTASVQLMAYNACGSATITHALNIPACKLSASNFNDLQEGILIYPNPASDFI
                     TIKLPEDLSSDLKVELYTSEGSLVSRSTINSTQLLSLPPSAGLYFIRVISAKQSQTLK
                     LVVE"
     gene            23030..25258
                     /locus_tag="PKY09_02025"
     CDS             23030..25258
                     /locus_tag="PKY09_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNB11660.1"
                     /translation="MTNKKKRGVFRMFVLLMWVMALLPLAIIVMVLGAVNMGKFGELP
                     TFEELENPNSSLASEVISSDNVLLGKYYIQNRSNIHYKELAENTVEALKATEDIRFEE
                     HSGVDMRGLGRVFFKTVILQQRSSGGGSTITQQLAKNLFPRENESGLRLALRKVKEWI
                     IAVRLERNYTKQEIMAMYLNTVEFGHNSYGIKSAAKTFFNKMPSQLNVEESAMLVGLL
                     QAPTRYSPVRNPKNALARRNTVLSQMEKYKFLSEAAYDSIVKLPIKLNFRAEDHNDGL
                     APYFRESLRLELVKWCKEHKKSDGSSYNLYRDGLRIYTTIDSRLQEYAEAGMKEHLTE
                     LQKTFYQHWKGRIPWAQHPEVIEEGIKKSDRYISLKAEGMSEQEIKENFATKIPMTVF
                     SWKGDIDTMMSPLDSLKYYKMFLQAGVMSIEPQTGYVRAWVGGNNYRYFKYDHVKAGR
                     RQVGSTFKPFLYTLAMQEGYSPCFKVPNVRVTIPIPGQPDWSPSNSDGKYGGMLTLKE
                     ALAESVNSVSAYLMKQFGPKPMIEIARKMGITSPIDEVPSICLGTPDVSLFEMCGAYG
                     TFANKGVWTEPIYITRIEDKNGMVLQEFVPRKVEAISEETAYLMLNLMQGVVQGGTGA
                     RLRFRFGLTNPIAGKTGTTQNQSDGWFMGITPTLVTGVWVGCEDRSVHFRTTSLGQGA
                     NTALPIWALYMKKAYADKDLNLFNGDFEKPEHPLSIETDCSKYEQDRKDHQHNSGFGN
                     DL"
     gene            25333..27774
                     /gene="pheT"
                     /locus_tag="PKY09_02030"
     CDS             25333..27774
                     /gene="pheT"
                     /locus_tag="PKY09_02030"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNB11661.1"
                     /translation="MKISLDWLKDYIKLQQTPEEIEYLLTSSGLEVESLDETDSIKGG
                     LRGLVVGEVLEKEKHPDADRLSVTKVNVGKAMPLQIVCGAANVAKGQKVLVALNGARL
                     YPTFGEPFDIKNSKIRGQASEGMICSEDEIGLGNSHEGIMVLDANAVPGTPAAEWFKI
                     KQDHIFEIGLTPNRIDAASHGGVARDLAAVINARAKAENNNSITAQFSMPDVSAFKPE
                     SNALHIDVVVDKNTGCGRYCGVAISGVTVGESPEWLKSRLQSIGVKCINNIVDVTNFV
                     MFETGQPLHAFDAARVKGKIIVQKTGKASTFTTLDHIKRKLTGDEVMICNAIEPLCIA
                     GVMGGTDSGVSASTTQIFLESAWFNAAEVRKAAKHHGLKTESSFRFERGTDPNGTLYA
                     LKRAALLIKEVAGGKIASSVVDQYPQVVEPQIIDINFEKINKLSGINFSNDMLLSVLE
                     YCGFSIKGSSANGATISIPTYKTDVTRQADVVEEILRTYGYHHIPLNEKTGMSFSSHT
                     PDRKPAMQFKISHHLSSVGFFEMINNSLVSSSHSAAVDETIKVLNPLSKELDILRPDM
                     IASTLNTICYNINRKNHNLKLFEFGKTYLQHDGKLTETNNLVIAMTGMVNEPNWHKSE
                     KESSLFYLKSVAEKAIELVMPRISSHLSWVQDSHTMLSSYTNIMQRQSAFGYIGSVKK
                     NVLKQFDISQPVFIAVLNMDVLLEKSLSHKTAIKLPQPFPEVKRDLSLMLDQTINFSD
                     LRDLALKQERKLLRQVDIFDVYEGEKIEAGKKSYALSFTLADDEATLTDKQIESVMQK
                     IEKAFEKNLGAVIRK"
     gene            27774..28544
                     /locus_tag="PKY09_02035"
     CDS             27774..28544
                     /locus_tag="PKY09_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB11662.1"
                     /translation="MSLDLFYRSSGNGQPLIILHGLFGLNDNWQSFSKQITENDIAVY
                     AVDLRNHGSSPHDPVFDYDSMADDIFAFIRTHDLKDINIMGHSLGGKVAMKFALEHQE
                     ILKSLIVVDIAPRYYAPHHQQIIKALQAVDLDTVNTRKEVEHILSGYIDDAGVKMFLL
                     KNLYWEGDRLQWRFNLKVIAEKIESVGQRITSTEKFEGKTLFIKGEKSKYITDDDKQE
                     ILSLFPHAEIKTCAHAGHWVHADNPECMLTTVRDFLSQ"
     gene            28603..31371
                     /gene="leuS"
                     /locus_tag="PKY09_02040"
     CDS             28603..31371
                     /gene="leuS"
                     /locus_tag="PKY09_02040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNB11663.1"
                     /translation="MDYQFRETEKKWQQYWAANKTFKVENKSDKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRFKRLNGFNVLHPMGYDSFGLPAEQYAIQTGQHPAITTEA
                     NINRYREQLDKMGFSFDWDREVRTSDPAYYKWTQWIFLKLFNAWYNNKRNCAESIDTL
                     ISEFEKNGNNDIRAVHDEIENFTAAQWKSFDEAEKSRILMAYRLAYLGEAYVNWCPAL
                     GTVLANDEVKDGLSERGGHPVERKLMKQWSMRITAYAERLLQGLEDIDWSDSLKEAQR
                     NWIGKSEGTTIKFSLQDSAEFIEVFTTRPDTLFGVSFVTLAPEHELVDKITSSSQRAE
                     VQDYVTKTKNRSERERQAEVTRISGVFTGSYVIHPFTGNKIPVWIGDYVLAGYGTGAV
                     MAVPAHDERDFRFATHFGLERPQVIEPHITHNFANASWDNKEGILINSDFLNGLDVKT
                     AISQCITEIEKQNLGKGKTNYRLRDAVFGRQRYWGEPIPIYYKENIPVAIPENELPLV
                     LPEVDKFLPTETGEPPLARAKNWKYKNQFPLETTTMPGWAGSSWYFLRYMDAINSTSF
                     ASKDALNYWTNVDLYIGGSEHATGHLLYVRFWTKALKDLGYINIDEPAQKLINQGMIQ
                     GTSCMVHRDVAGNKIISAGLKSNYNTTLIHIDVNLVDNTEVDIEGLNKWRDDFATAEY
                     VTENGKLICETAVEKMSKRWYNVVTPDSICEKYGADCLRMYEMFLGPLEQSKPWNTNG
                     IAGVSNFIRKLWKLYHNNGKFEVSNEAATKAELKTLHKTIKKVQEDVEKFSFNTSVST
                     FMICVNELTEHKCNKREVLEPLAIILSPYAPHIAEELWHLLGHNTSVSFATFPEWNET
                     YLIENEFDYPVSVNGKTRFKILLPLNIGKEEIERHVMASDEMQKWTNGNAPKKVIVVP
                     GRIVNVVI"
     gene            complement(31808..34072)
                     /locus_tag="PKY09_02045"
     CDS             complement(31808..34072)
                     /locus_tag="PKY09_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB11664.1"
                     /translation="MNHLPHLIVDLALVLLAGSATILLFKRIKQPLVLGYIIAGFLVG
                     PHFKLFPTVIDIESIDVVAELGVIFLLFGLGLEFSFKKLMRVGGSASITAFVEIAFIT
                     ITGFLLGRWLKWSVIDSLFLGGMLASSSTTIIIKAFDELGVKTKQFARVVFGVLIVED
                     IVVILLMVLLSTIAVTQQIEGGEMVFIVLKLLFFLSLWFIMGIFLIPTLLKNAKKWLD
                     DENLLILSIGLCLGMVVLAVEAGFSAELGAFIMGSILAETTSAEKVEHLTKPVKDLFG
                     AVFFVSVGMMIDPQAIIDHKWAVVAVTLLTLFGKIISTSIGALISGQPLKQSLQVGFS
                     MAQIGEFAFIVAALGASLKVTSDFLFPVAVGASAITTFTTPYLIKYTEPIYMQIEKLL
                     PSRWVEYLNRFSSGTQKIQSATGWQSLLAAYSRIVIINGIILLALGLLLDRILIPYFN
                     TIQVNEIVKAIAVPAISLAAGAPFIWAIVARKPNHPYYKNLWLSKKEFRTGPLLIIEL
                     TRVAMGVALITGWALYFFKPIIALVVVLPITLIIFRVFAMHLQKFYKRIEGRFITNIS
                     ERESVEYHSTSASLNRKNYEVQSFLSPYNAHIVQLEVPANAAFIGKELWQLAWREKYG
                     INIVYIRRGDKIYHIPGRNQLLLPFDEVGVLTTDEQIQVFKTVFENPIEEDAEPTSLD
                     DIGVFQLAITEHHWLNGKSIRSSGIREKALGLVAGIERNGERILNPDSTTIFQTDDLV
                     WLVGNVKKMKELKI"
     gene            complement(34112..34291)
                     /locus_tag="PKY09_02050"
                     /pseudo
     CDS             complement(34112..34291)
                     /locus_tag="PKY09_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
     gene            complement(35141..35626)
                     /locus_tag="PKY09_02055"
     CDS             complement(35141..35626)
                     /locus_tag="PKY09_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11665.1"
                     /translation="MKTVLAFILIVFLTSCRKEEKYPVEPVLTFKAVDFYGKNSFYLI
                     TTFTDGDGDIGLSDADTLPPFDKNSHFYHNYFCNYQQLINGNWTPVNFNIPYYYRIPI
                     LTPKQRNKNIKGDIKVDVQNFLSFTTNGVADTFRVEVSVADRALHESNKIISDYFIIS
                     K"
     gene            complement(35639..36157)
                     /locus_tag="PKY09_02060"
     CDS             complement(35639..36157)
                     /locus_tag="PKY09_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB11666.1"
                     /translation="MSKTSTIFIIDDDPMMQNMLKDHLSAMSNFTIHTFATGEEALAS
                     GQLVPDIVFLDYNLDSKIANAMDGIDVLREIKAKYPTTEVVMLSGQDKIEVAVDTMKY
                     GAFDYIVKGESAFYRAEKTVFNLFKLAQLKRNAGMYKVLTITFGVAFLIMIIVVVILI
                     SKGMISDHVGWF"
     gene            complement(36194..36541)
                     /locus_tag="PKY09_02065"
     CDS             complement(36194..36541)
                     /locus_tag="PKY09_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNB11667.1"
                     /translation="MEKVTNLDFIQKFSKGDMSRVKKYVMMYIQSADEEMPVIEKSFH
                     DKDYKKLKTASHTLKSIAAYMGMERAQQLLKKIEDSASNESVTEEMAHDITQLKEMIT
                     LSKDELKAFIAQN"
     gene            complement(36555..37520)
                     /locus_tag="PKY09_02070"
     CDS             complement(36555..37520)
                     /locus_tag="PKY09_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNB11668.1"
                     /translation="MTSVFDTPFDFESVALESFRFQAENNAVYHNYLKLLNINPLKID
                     SISKIPFLPISFFKTKKVVTGNIDSSAIVFSSSRTTGTEPSLHYVSDISLYEKAFTQA
                     FNHFYTSTDQWCILALLPSYLEREGSSLVYMCQHLVEKTGKKNSGFYLNDFRKLADTI
                     TVNEQRGIKTLLIGVSFALLDFAHAYPMPLKNTVIMETGGMKGRRKEMIRQELHAILK
                     EAFLLNVIHSEYGMTELLSQAYSSGNGIFHCPPWMKVFVRDSNDPLSESIPGQGGINI
                     IDLANINSCCFISTQDIGRVYDDGTFEILGRFENAEVRGCNLLIE"
     gene            complement(37537..39093)
                     /gene="nadB"
                     /locus_tag="PKY09_02075"
     CDS             complement(37537..39093)
                     /gene="nadB"
                     /locus_tag="PKY09_02075"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNB11669.1"
                     /translation="MTKTDFLVIGSGIAGLSFALKAAKHGKVTIITKANEDESNTKYA
                     QGGIAAVLPEAKDDSLEHHVQDTLSCGAGLCDEKIVRIVVAESGERINELIAWGTEFD
                     KNKSGSFDLAMEGGHSAHRVLHHKDITGLEIETKLLQQIHQHPNITLHTHLFAVDIIT
                     QHHMGVIVKRNTPGITCYGAYILNTKTGKVETMLAKVTVMATGGAGQVYKTTTNPVIA
                     TGDGIAMAYRAMGKVNGMEFIQFHPTALYHPGTYPSFLISEAVRGFGAVLRTIDGKEF
                     MQRYDERGSLAPRDIVARAIDSEMKKSGDDYVWLDCTKLNIIEFKKHFPNIYNRCMSL
                     GIDVAKDCIPVAPAQHYLCGGIKVDEHGQSSIQHLYAIGECSSTGLHGGNRLASNSLL
                     EAVVFAHRAYVHASERLEFIHYNEHIPEWNAVSPREVAEMVLITQSRKELQEIMSSYV
                     GIFRSDMRLKRALERTRLLYEETEELYNRWEISPRICELRNLINLSYLIIKAAMARTE
                     NKGLHFNVDN"
     gene            39170..39799
                     /locus_tag="PKY09_02080"
     CDS             39170..39799
                     /locus_tag="PKY09_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNB11670.1"
                     /translation="MSTRNHQVYAELTPNPNSVKFVADRLLLFEGTVEYKSKAEAIDC
                     PIAYQLFDFTGIKSVFITTNFITITKEADIDWYEITNILREFIKGFLNSGEKLFLKNP
                     VQEIEEVQVPTSSNIVTPQEKQPLQNNTESAIIEDKIKNMLDEFVRPAVEGDGGAIDF
                     KSFKDGVVTVVLKGSCSGCPSSTLTLKAGIENLLKRMIPEVSEVMAEAE"
     gene            complement(39802..40473)
                     /locus_tag="PKY09_02085"
     CDS             complement(39802..40473)
                     /locus_tag="PKY09_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB11671.1"
                     /translation="MIKIALVDDNSINQKYFGEKIAVFGDIALLFVSNSGDEFLAKMK
                     GLPMQNWPQVVFMDIQMPGLNGVETIQLAKALYSSIHFIVLTVFEDDETIFNAIQSGA
                     SGYLLKHENYLSIKEAITEVLEFGGAPMSPAIARKTLGLLSQRDNSKPAKGSVIETLL
                     SDREREVLTHLVNGYDAKRIAEITGISTLTVRKHIANIYTKLHVNSKAQVISMAHKNK
                     WFNIF"
     gene            complement(40529..42442)
                     /locus_tag="PKY09_02090"
     CDS             complement(40529..42442)
                     /locus_tag="PKY09_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11672.1"
                     /translation="MKKAIRCFSVLVLILVQKLYYTHAQTPITDSLKQKIHSSTQLNE
                     QLDNVIKLCARGHSLSADTFYYFIRLGQQIALPGSPEYFLVENAYVRYLAKVGKTKEA
                     DFLVDSLLKVIPQNEKYYDASQWLRSNRSNIYIHTSRQKEAINEGFIILKNAEKANDA
                     YWIARSYIGIGWANMELGKYDDAIKWLSTGINSTNDEKILVRCNYLFSNIASCYNNIG
                     MQDSAFHFIELGLKYARLAQNLSQVANSLNIRADIYIKQKSFLKAATDLEEALKIRQQ
                     IGDKIYVVADMAQLSSFYASINETKKGIEVAKEAIAIATKLKSLSRLIFIYNSLAENY
                     KAAGMYNEYAQVLEKIIKLKDSLSEKNSAEAIANFETKYELQKKENIIVQQEMRISRN
                     RYVTIGSLLLLALISLILGLVYRNRQLIKNRKMEMDLVEQRVQAKVAVKQAQENERKR
                     IAADLHDNIGVYAAAITHNVRSLKSDDNSMQTITENLEENVQSMVAQLNNTIWVLKNE
                     QLSLISLSDRFKLWMQRLMKSYTGVTYQFSENIVNDRVYTPSVMLNLFYSLIECVNNS
                     LKHSGCTAIKIEFNNNGSSSIIVEDNGKGMSENIVYGNGIMQVKQRMKESGWTVQWIP
                     VEPHGTRIIFTNT"
     gene            42763..48207
                     /locus_tag="PKY09_02095"
     CDS             42763..48207
                     /locus_tag="PKY09_02095"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNB11673.1"
                     /translation="MTTKIAKRNWMFSLPRVNKLQLIWLFLFLSGIAMAETEPNDSKA
                     QANVLTLNSGMPGTVTLTESDWFTVTIPEDGNLNIQGIFSSNIYIQIYDNDGVTSLFN
                     NYHSGTLTRTVNGLAAGTYYIRLYNYYSSETNTYTITATLTTTGLANDIEYNGTPATA
                     SVLALNGSTTGHVNYYYNNQKDTSDWYSVAVNQDGKLSYTITSNGQNVYAILYDGDAT
                     TQLAGSYTTATATFQRDGLAPGVYYIRIYTYYSSEYVAYTLSNILTPPVNANDPLPND
                     VYTQATTLPFNGSITGHIGFRYNGAIDTYDWYKVTTNADGKIQYTLTVDNGQHVYAEL
                     FDGDGTTFLEGSYTTTSATFEEDGQAPGTYYIRIRTFYSNQFASYQLSNNLVVPVQAN
                     DASPNDVYTSATVLNLNDSTTGHIGYRYNGNIDSYDWYSVTTNANGQLFWTITSHNGQ
                     NVYAELFDGDGTTNLAGSYTTTTATFNKNGLAPGTYYIRIRTFYSNEFSPYTLSNSLS
                     PAVYTNDIEPNGSKPTALPLAVNTTVQGHIGFYYNNLDDTLDYYALTLPVDGKLSWTI
                     SSANGQNVYAQLYDNDGTTNLGGSYTTTSATFTENNLAAGTYYIKIRTFYISEFASYE
                     LSTSLDPMNFTVENTTDNDFAITPTLLPANTPTTGHLNFYYNNQYDNKDWWVIGYDGT
                     GAMTITGELEQNHFDGDYPYINYALYTDTAASAIASGTWHNPTNSFMVNTPVIGKYFL
                     RLTPGFSTFGAYRLTAGYTENCSNTITINSAVQAPPCLGTINYTVSNGLGNYTVQLYK
                     NGIPFGTPQTTASTVSFNNLGIGTYYLQSSSFGASGACSANSANTVFSTPPVPNITAG
                     GPTTFCQGGSVVLTADAANSYLWSTGATTQAITVSATGNYVVTVYNAALCSNTSASTS
                     VTVNPLPPTPTITPNGPLTFCTNSNVTLSSSVANDYLWSTAEITQSIVVNLAGTYSVT
                     ISDANGCTATSASVTTNTIALQTFYLDADGDSYGDATNSIQDCAAPSGYVSNSTDCND
                     ANNAIHPGATELCNGIDDDCNGLVDDNIVNSTYYADADGDGFGNPLVDSSACAPPIGY
                     VLNNTDCNDANNAIYPGATEVCGNGIDDDCDGQIDEGCGSNTWYADADADTYGDINNT
                     VVDNNPTPPSGYVADSTDCNDANAAIHPGATEIVCNGIDENCNGMADDLGTPSTDPTS
                     ISSDVVFNEICIGGNTTLTVNGGSLGTGASWQWYSASCGGTPVGSGNSITVSPATATT
                     YFVRAESPCGNSACVSLTITIKTSLPAHAVVVPPISGLPANACPGSTANISIPAVANA
                     SQYIWDGPSGTYFNGNPLNTSPFTSSTPSVQVTFGTTNSSFYNIGVQAANACGATLRK
                     IQKVRYKISTPAAISGAVNACANSSNSYAIAAVTGATQYLWSITGDATVIGNGTTVTV
                     NFGPAWTGGNLCVSAQTTCFTSPAKCMLISASSTALNAINGTFTACPGSVISYNVAPS
                     AGASYYTWTLPAGATGSSTTNSINVTYGPSFNSAGNICVTVTSACGVTSAPKCKTVAP
                     GLPARPLAITGPSNGLCGQSASYSVVSTPGITYNWTAPGSISGNGTNAITVAYGSFTT
                     GSVCVTASNSCGTSAARCITIKGAPNTPVALTANPATWCANTQGIEFTANVSNTTGSY
                     TLAWTYPSSPVATYISGGGNSNTLTLDWGTGNGTVIVKASNSCGEGSKAYLVNIGCKE
                     AQQVDAGTLSAYPNPTAGMLNIEFNAVEKSNASVSVIDLSGRTIMNSSQTVTAGSNNL
                     QLDLTNVAKGAYMIRVKANQFTKQIKVIVE"
     gene            48298..49629
                     /locus_tag="PKY09_02100"
     CDS             48298..49629
                     /locus_tag="PKY09_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNB11674.1"
                     /translation="MRASILLTVNLVFTTFAGFAQWSTTTVQSARSLLAATSIQDKAM
                     FGGGIGMISVPPPALDIYTQSTNQWMQSQISQGRTSLAAASTGNWAIFGGGANDWILS
                     TYNRVDIYNGTVGSWTDGDFLSVNRAKLAAAGAGSKILFAGGEFDMINAVYDTVDIFD
                     TLTQSWSTSALSVARFNLAGASAGNKILFAGGEDLTGTVYDNVDIYDVTSGIWTTANL
                     SVPRTRLAATAMGNKVYFAGGINNIGVVSNVVDIYDVISNTWTTDTLSQARQYLTAAA
                     TMGKAFFAGGTDAGLLTGSNIVDIFDTATATWSIDSLTTGRYLCAATTLGNQFLISGG
                     IPSGTGFPLNTVEIYTIATSGISPIETSKTLSVYPNPASQQIMIKYDDFKFGDVLNIT
                     DVMGKHLLSLQITNNETFADISNLSDGIYFVRLNSGLRNIASKSQKIIIHH"
     gene            49909..50361
                     /locus_tag="PKY09_02105"
     CDS             49909..50361
                     /locus_tag="PKY09_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11675.1"
                     /translation="MENRNNKNYVQYNLARMAFILFALMTGLQLQAQTPPFTRSTFQS
                     AYVPISIGGGATSSTATGDNTMQTGIPIGFNFGYGDSTFSSISLNTNGLTWFDTPAPN
                     PAAGHFFIATNTAPNQTLWAWANDDLIDDASSDILYQILVLLLELNGA"
     gene            50418..>51858
                     /locus_tag="PKY09_02110"
     CDS             50418..>51858
                     /locus_tag="PKY09_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11676.1"
                     /translation="MLSPFSGWPAYNFRFTPGAPSTIPAGTYNVGVGQTYNSLTQAVA
                     DINHRGINGSVTLNLTDALYDTTAANGSNIFPIFVATPNADSINTLTITKIGTPATLA
                     YRGSSITSTPVMAFGTAIGLAALNHDMEPILGVCGSYTTVSNINLISHGSPQVVDYGL
                     LQFDMAEGKGAQHNLYDKITVDLDRNYGDAHGMYSVATGGGWNNTNNSYNTWRDITIK
                     DCNYGFRISGAYYSTEVGNQLITSSCNTYNYIGDPNTPDDIISSLNNYGVWITKVAGF
                     TMRNCIVQNITTTSTIGIQAVRIDGAINGISEISNNIIRTIKRINPQIFPTNSIDGMY
                     IDWGNNTGLFKVFNNSISNLLTTYAGAPTDVVATRGFVFGDGGAGPYGVDLFNNSVSI
                     DGSTFPNASNLCLETFSNDATFTIKNNVFANFTTGQTGVATHTCFFTQYTDHYGNAAS
                     ISDYNNYYVADTANGAPFKATTTMYSTQAA"
BASE COUNT        16331 a        10083 c         9512 g        15932 t
ORIGIN      
        1 ttagccattg atgctaccaa cataatgcgc aaacacagtg agttttttaa tgctgattta
       61 gtaaactacc tgcacgttga tgaagatccc tttgggttta gtaatctgaa atatgtccgt
      121 aaagttgaag agtcgaaaaa aattaatgat ctgaaaggac cttgcatcat catttctgct
      181 tccggaatga ttgaagccgg aagaatcaaa catcatatta agaacaacat caatgatagt
      241 cgaaacacca tacttattgt cggctactgc acaccggaat ctattggcgg gcatttgatg
      301 cgtggagata agataattaa aatttttggc aatgaatacc ctgtaaaagc taatgttgaa
      361 gtaatttcat ctttcagtgc acatgccgat tatcttgagt tgataaaata cctttcttgt
      421 caagatacag cagcagttaa aaaacttttt ctggtgcatg gcgaatacga agttcaacaa
      481 gagtttaaag agaaattaaa agaggttggt tttaaaaatg ttgaaattcc cgaagaactg
      541 caatccttta aactttaaga ttacgcaaga aagtaatcat tttgccaaca gcaatttgcc
      601 tgtgactgat tttatttttt tcgtcctcag tcatttctgc aaaggttacc tcatatccat
      661 tgggtataaa aaccggatca tagccaaatc ctgatttccc atgttcaaca ttagtgatat
      721 tgccatctat tctgccttca aaataatagg ctttaccttc ccaaatcaag caaataactg
      781 caataaaata tgcatttctt tgttttatac cttcaaggtc tgccaacaat ttatttctgt
      841 tatcagcatc agtagcttca tgtccggcat atcgtgccga atacacaccc ggtgcatcac
      901 ccaaagcatc aacacataga ccgctatcat cagcaaaaca attcttctta gtatgcaaat
      961 aaacggcatc agccttcaga aaggcatttt cataaaaagt tacaccggtc tcatcagcag
     1021 atgacttaat tcctgcctct gaaagtgact gcaaaatgta accggaaggc agcattttac
     1081 gtatctcttg taatttatgc ttattgttag aacaaaatat taactcagtc attttttaag
     1141 tcaaaaatat atacatttac tttgttttaa aaaccacctt aaaatgcaga aaatattttt
     1201 tgctttatcg ctgttattca gtctaagcag aaatgcacaa gctcaatgcc ctaatgcttc
     1261 attttcattg cccgatactg tttgcagcgg cactatgctt ccattgatta atacatcaac
     1321 aggaaacgga ttaacctatc aatgggactt ctgccctcgt gaaattcatg gtttaacatc
     1381 ctatagatat ttcagatata acaattatgt cttaaataca atgactgata tggatttact
     1441 gaaagttgga aatggttatt atctgataac aatggatagc ataagtaata attttgtagt
     1501 aggcaatatt ggtaaccacc tttcaaatca aacattcgat gcaataatta atagtggttt
     1561 tacgaaatta aactgctttg actttatagt tgaaggtgac accaatgtgt acggattggc
     1621 tgcctatgct ccaaccagca cactatactt aatggctttt cccggtggca ttcaggtagc
     1681 acctgtttta actccactgc caacattcag tacagttgca aaccctgcag atattaagat
     1741 agttcatgct gataatattt attatgcctt tgtagcaagt aaaggaaatg gaaatgttac
     1801 ctgtctcaca tttggtaact cactgataaa tagtcctaca gaattatata gccttaatat
     1861 tcccggaagc attaaattat cagacattga tattgtaaca acttgttcgc cacctgttgg
     1921 ttttgttagc gattcaaaaa atggtgatct tactaagcta tcattcacca atggatttag
     1981 taatgcaccc accttgtctg tctattcagg tacaggttcc attggtaaca actcagttgt
     2041 gattacacag gaatttgaaa gctattttgt ctgttataca gatacggcaa ataaacaatt
     2101 gaaatggctt cagtttgatg ataataactt cgctaataca ccaaccgtct atacatcacc
     2161 tgctttttat tttggtccta accaagcaga atcacttact gattcatcaa ttacaagatt
     2221 tattgtaaat gacgctccta ccctcaactt tgttgaaatg ctttactttg atagttgcta
     2281 tacaaaatac agtgaagatc aaaatccttc tttcagctat ttaaataccg gatggaataa
     2341 attatcattg accgtttctg attcaaacgg atatcaatcc attgcttacg atagtgtgtt
     2401 tgttattacc ggacctaaat cgcagtttag ctattcaggc aacacttgct ttaatcctaa
     2461 tgatagtata acatttaatg atcaatcaac ttcaccctcc ggagcaatta cagattggtt
     2521 gtgggatttt ggtgatgggg ccacctccac acaacaaaat ccacaacatg catttacatc
     2581 acccggtacc tatcaggtta aacttaccat cactgccgga tgtgaaaaag atacgataat
     2641 accaataaca attcatgcat tacccattgt caactttaca tacagtttat cgtgtgccaa
     2701 ttctgcaaca acattaacag atcaatcatt ctctgcagca ggcttacagt cgtggtcgtg
     2761 gaattttggt gacggcagca gtaacaatca acttcaaaat cccgatcata tatttcagtc
     2821 aggtggtaat tataatgtgc aactcactgt aaccgatagt tcgggatgca ttgattccat
     2881 agtcaacatt gtacaggtaa aagcaacacc atctactgca tttatggcat tgcaaacatg
     2941 ccttggtgac acagtacaac tcagtaacac ctcaacaata agtgacagta ctgcactcac
     3001 ctacttttgg gattttggca gtggcattac ttcaacacaa acagatactg taatttattt
     3061 tacttcagca ggtagctatc cggcaacatt aattgcatat tcatcacaag gatgttcaga
     3121 cacactcaat cagaatatag ttgtttcaac accaccttcg gtaaactttg gttttccatc
     3181 aagcaactgt caatacaatg ccattacttt cagtgacagc accgttggtt ttaacctgtc
     3241 ttcatggcaa tgggatttcg gtgacggtga tacttcttcg cttcaatcgc ctacacatgt
     3301 ttacaacctg tcaggtaatt atactgtgca acttactgtt tcgtcaggta acgactgtgc
     3361 ggcatcctat tcacaaacaa tcaatatact tgaagggcca tctgcagcat tcctgacagg
     3421 caatggatgt cagggagttt caaccaactt tacagatcaa tcaactgtaa gcagtggttc
     3481 cagtattgtg gcatggcagt ggcattatgg tgatggtgat tcttcacaaa tacaaaaccc
     3541 ttcacacatt tacgctgctg ccggaagtta taatgtcttg ttgcgtgtaa catcagacaa
     3601 cggttgccaa gacacaacat cacaacctgt tcaagtcttt gaaaatccaa ttgcaggttt
     3661 ctatgtacaa ccattacgtt gtgctaacac agaaatttac tttcttgaca cgagctctat
     3721 aggtaatggt aatatttctc aatggcaatg gcaattcagc aatggtacaa caagcaatct
     3781 tcccaatccg tttgctattt tcaacaatgc aggaagtgct tcagcaacat tgacagtgat
     3841 tacatcagat ggttgcagca atagcaattc atttaatttt actgtaaggc cacagccaga
     3901 ttttaccata gcgcataatg gtgtttgtaa aggaaaacaa acttctttta attactttcc
     3961 gaatggaaat atatctgcct atgcctggac atggtatttt ggcgacaaca cttatgcttt
     4021 tacaccaaat gccacacact tatatgcaac ctcaggaaca tatccgtttt caatggcagt
     4081 aacagattca ttcggttgtc aaaaaaccat attcgacaca ttgacaattt atcctactcc
     4141 cgatgctgca tttatcacga atggtatttg tcagaatgct ccggttgatt ttataaacca
     4201 aacagacagt actaatacac agatcaacgg atggcaatgg agttttggtg atggcaacag
     4261 ctcttcacag cattcatctc aacacattta taacggtaca ggcaactata cagtatcatt
     4321 aatagcctat gcagccggag gttgtaacga caccgttttt tcagtagtga acatcaaacc
     4381 agtacccgaa attgcattta gtatgctgcc accaacaggt gctccaggca ataatgttca
     4441 ggtatttaat aatacaaccg gtgcaacatc atattcctgg gattttggtg atggctcacc
     4501 attgaatctt aatgagaatc caacacatgt ttataatgat acaggcagct atgttattac
     4561 actaacagct tcaagtattt ttaattgtat ggcgcaattg caaaaacctt atgatgttat
     4621 tgttccctat gttgaccttt ggctaaaatc attgtcctat accattaaca ataattatct
     4681 gtcattatcg gcattgctgt caaatattgg aaacacaact atcaataatt ttgatttatt
     4741 gatacagcct gaagggaaag gttctttcat agaaaatggc aatgaaacaa ttccttttgc
     4801 aacagaaaaa aattatgcct tacatacaag tatttctatt aatccatccg acctgcctga
     4861 atttatttgt gttcgcataa aaaatgtaaa taatggcaat gatctgaatc ctgataacaa
     4921 cgaaaaatgc atttcattaa tgccggagaa tggaagtatg tctgtaagtc caaatccaat
     4981 tgaagatgta ttatccattc atctgaattt acctgcaagt tcagaaataa ccttagtgct
     5041 ttatgatgcc acaggtaaaa aactgaccac actttatcag ggcactactg atgccggttt
     5101 caaaaacttt agctttaagc ttccttatct gagcaaaggc gtttacataa taggagctac
     5161 atcagaatca ttaaataaaa tattaaaatt tatcagacag taaaattatg attccattca
     5221 ataaacccca tcttactggc aaggagacag actacataaa agaagtattt gcatcaggaa
     5281 aaatttcagg agatggaaac tttaccaaac gatgccatca gtttctacaa cagaaatata
     5341 actttaaaaa agtattgctt acaacatcat gcaccgatgc attagaagca tgcgctatat
     5401 tgcttgatat aaaagaaggc gatgaagtta tagtgccatc ctatacattt gtttcaacag
     5461 taaatgcttt tgtactgcgt ggtgctaaaa ttatttttgc agatagcaat cctgatacag
     5521 caaatattga tgtaagtaaa attgaatcac tgattacttc gcgcacaaaa gcaattgtac
     5581 ctgtacacta tgccggtgtt gcctgcgaca tggacagtat taatgccatt gcagcaaaac
     5641 ataacttata tgttgttgaa gattctgcac aggcgattga ttctttttac aaaggaaaac
     5701 cactgggaag tttaggtcat ttgtcggcat tttcttttca tgacacaaaa aacatcataa
     5761 gcggtgaagg tggcatgttg gtcatcaatg atgaacactt tgagaaacgt gctgaaatta
     5821 tccgcgagaa aggtactaac cgttctgcat tcttcagagg tgaagtggat aaatatggtt
     5881 gggttgatgt aggttcatct tttttgccaa gcgaagttac agcagctatg ttgttggcac
     5941 aattagaatc aattgataaa attcaaacca gaagaaaaga aatttggaat cgttattata
     6001 gtttacttaa atcattagaa gaaaaagaat ttttaaaact tcctgtcatt ccggattatg
     6061 ctaccaacaa tgcacacatg ttttttatcg tcttaaacaa tctggaaacc agaagcaagt
     6121 tgattgatca tcttaaaagg aataatatca gtgctgtatt tcattacatc tccttacatg
     6181 catctgcata ctatgtcagt aagcatgatg gacgtgcatt gccggtttca gatatgctga
     6241 ccaatcagct attgcgttta cctatgtttt atgatttaaa aaatgacgaa gtggatttca
     6301 tttgtcagaa aataacagat ttttttacaa cactttgaaa agaattgcgc tgctatcaga
     6361 cacacatggg ttcattgatc agaccattgt aaaatattta caggatgcag acgaaatatg
     6421 gcatgccggt gattttggca ctttagctgt aagtgatttt ttagcttctt taaaaccatt
     6481 aaagggagtt tacggtaatg ttgatggcca cgatgtaaga aaagttcatc cagctcatca
     6541 gaaatttcaa ttagaaggga ttaaagtctg gatgacacat attggtggct atcccggaaa
     6601 ttattctcct gatataaaga aaaccattta tacagaaaag ccaatgctat ttatttgcgg
     6661 acattcacat attcttaaag taatgcctga tcctaaaatt ccgggaatgt tacacatcaa
     6721 tccgggtgca gccggtgttc atggatttca tcatatacgc acaatggtaa agttcattat
     6781 tgataaaacc aaaataagtg agcttgatgt aattgaactc ggaaaaagag cctcaataga
     6841 aaataaaaat attttacctt aaacggtcag cagaacgcac caaatcttcg tcttttttaa
     6901 tggcctttga agccaagaag acccaaacta ttgagattac tgtaatatat gtcccaacat
     6961 gatatactac atgagtaaca tcagttgccc ttgtttctga ctgttgaaaa atcaaaacaa
     7021 caagtaccac aatcaacagc ataattaatt gacaaagtct gatttgcttt aggcggtgtt
     7081 tatataaaaa caaattcaat aagcttaaaa caattactac cacggtcaga atcagaatag
     7141 ggtaaaatgg tgcattttgg gtctgaacgt tatcctgaaa agttttatgt cccataactt
     7201 caaagacatg catagaagtt atactgtttt ctgtggtttc taccgagcta aaaggaagaa
     7261 aaaacaagct ggccatactg gcaactacca aaaaaagata aactgattga atccgttgaa
     7321 tcatgtttct gaaattttta tgtgtcaaaa ataggttaag tggtgaaatt atgctttttg
     7381 aagaatcttt tttgcaaaag aaaaataatt gttttacgtt tgcgcagaat ttgaacgaaa
     7441 gccaaagcat ttttccccgt tcaaaagtcc gattccaaca atattttatc atccacaaga
     7501 acataatttc ttttacctta tacatacaaa ttcatgtacg atattctcga acttagtgac
     7561 aagcttgtag ccgagctacg cgacattgcc aaagaactta acgttgaaaa ctacgaaggc
     7621 ttaaaaaagc aggatcttat ttacaagatt ctcgatcatc aggccattca tccggaatta
     7681 gcaaaatcta aaaaaggcga taagcccaaa gaagaaaaac cacgacatga gaaaccacgc
     7741 catgagaaaa aagaacacaa acctgctgct gcacctgccg ttacagaaaa acctgttgaa
     7801 gaatctgcaa ctacaaatgc taccacagaa gtaatatctg aagctaaagc tcaggatgat
     7861 gcagataaac ctagaagaaa gagaattatt cctctgggtg caccacaaaa aaatgaatcg
     7921 catcaacagg ttgccgcaaa aactcctgaa gctactcctg ctccacaaga aactgcaact
     7981 gctcctgcaa cagcaaaaac aaatgttgaa gaaaggccac aacaaaatca acagccacaa
     8041 caaaatcaac agccacagca aaaccaacaa aacgattttc agcaaagaaa cagagagcgc
     8101 aggtttccgg acaatcaaca acaaaataat ttccgtcagc aaaacgatta ctttgaattt
     8161 gacaatgcca ttacagcaga aggtgtttta gaaattatgc ctgaagggta tggctttttg
     8221 cgctcatcgg attatgacta tttaagttca ccggatgatg tgtatgttgg acaatcgcag
     8281 attaaagcat ttgcattaaa aacaggtgat actgtaaaat gtaaagttcg tccgccacgt
     8341 gaaggagaaa agtttttccc tcttactaag gttgaattga taaacggtct tgagcctaca
     8401 attgttcgtg accgtattcc atttgattat ctgacaccac tttttccatt cgagaaactt
     8461 aacctttcag gagagaacag caccatgagc acaagaatta tggatatgtt ttcacctatc
     8521 ggaaaaggac agcgtgggtt aattgttgcg caaccaaaaa caggtaagac ggtactctta
     8581 aaagaagttg ccaatgccat tgctgccaac catccggaag tttatcttat agtattgctt
     8641 attgatgaaa gacctgaaga ggttaccgat atggcacgca gtgtaaaagc cgaagttatt
     8701 tcttcaacct ttgatgagcc tgctgacaag cacgttaaaa ttgccaacct tgttttggaa
     8761 aaagccaagc gtttggttga atgtggacac gatgttgtaa ttgtattgga ttctataacc
     8821 cgattggcgc gtgcctacaa taccactgct ccggcatcag gaaaaatatt atccggtggt
     8881 gttgactcaa atgcgctgca taagccaaaa agattttttg gtgcagcacg taaaatagaa
     8941 aaaggtggtt cgttaactat cattgcaact gctttaattg acaccggctc gcggatggac
     9001 gaagttattt ttgaagaatt taaaggaaca ggtaacatgg agttgcagct tgaccgaaag
     9061 cttagcaaca aacgtgttta tcctgcaatt gacttagttg cttcaagtac aaggcgcgaa
     9121 gatcttttgt tagaccgaaa aatgttggag cgtatgtgga ttttacgaaa tcacttagcc
     9181 gatatgaacc caatggaagc aatggaattt ttactcgacc gcttgagagg aacaaaatca
     9241 aacgaagagt tccttatttc tatgaacagc tagaaaggtt agtgaatttg gaaattactg
     9301 cgatggagaa ttagtgattt gcagagcgtg aaatgtggaa gttaaaaatg tttctataat
     9361 aaagattatt cgaactttgc agcctgacta atataaccaa tttaccattt attattctgc
     9421 atttttcaat ttccaaactg atccgttttt ggttgtcagt ttttctaact taccatttgc
     9481 caccaaatca ttaagtactt gttcaccttg cgactttgct gtttctgtca gtacagaata
     9541 ttcttttgct gtcaatgact gatagtgtga aaagactgca gtcgtacttt tgtcataatt
     9601 gattttttgt accttaggca gaagtttaat gattacattt tcaaaggcct cataaggctt
     9661 tgatccatac acagtttctg tctttcccga tgtatctgta aaaaacatag tcgggaaacc
     9721 tcttacacct aattgttttc ctaaggttaa atcttcatta aaaagttctt ttgctcttcc
     9781 attgtaatct tctctaaact tatttaaatc taatcccaca gactttgctg ccgcttctaa
     9841 atgttcccac tttgttatat tccgtttttt tagaaaaacc atttctcgta tctctcttaa
     9901 aaagagaata gccttttcat gatcttgcat ttgggccgcc tttactgcaa tagaaggcgg
     9961 atatgaagat tgtaaagggt cttctaacca aacgtcaccg tcaattggca tatcataata
    10021 aacactaacc tcatcccaat ggtgagcaac atctgatggc ttacttattc ctccactatt
    10081 gtaactccag tcgggtaaca aaccacccat tctgtattca atttcaataa tgttaccgta
    10141 ctccaatttc aatttgcgta gctgaggttc aataccccaa caagacgaac aaatcgggtc
    10201 agtgtagtaa attactttta ttggtttctt ttctgatttt attttgttgc cttctgttac
    10261 tgtctgtcct tttgccggga tttcgcaaat gccgctatcc ggattgcata ataaatgatt
    10321 gttgttctcc atattctttt ttgtttgagc ctggcaattg atactaccga atgctaatat
    10381 tattacaatt gttaaataag tccaattcat tcttttattg tcttatttca tttttaatgc
    10441 ctgaatataa ataaggcact aagcatgatg ataccgatca atagtactat cagcaaccgt
    10501 ttatcaaaag gaggtgtttc gtttacaatt tgctttttaa gctgtgtgat gatatcactt
    10561 agtttgttga aactttccgg tgattttaca atgtaatcaa atgcgcccat cttaattgcc
    10621 gaagctgcta catcggggtc ttcttgtccg gtaaacatta ctacacgtac agttttattg
    10681 gcttgcctga tgtgtgaaag aacagttaat ccatccatag catctttatc gtaaagacta
    10741 aggaagtaat caagaataat taaatcggga cctattttca aatcaatgag tgcatcttcg
    10801 ccagtcttat aaaggttgat ttcaatatca ggaaaatgtt ctgtaagata atctacaatc
    10861 attttttgca gatgacggtc atcctcaaca acaaatacta aaaacttttt ctgcgtcatg
    10921 tgctttaaat atgcttgtct gctattaatc gtaagaattc acttcttgtc ctgtcgtttt
    10981 cgaattcacc agtaaatgca gatgttgttg ttatggaatt ttgtttttga atgccacgca
    11041 tttgcatgca aaggtgtctt gcttcaataa ctactgccac tcccagaggt gataaggtct
    11101 cctgaatggc atcgcgaatc tcaaccgtaa gtctttcttg cacctgcaat cttcgggcaa
    11161 aaacatcaac aacacgaggt atcttactaa gtcctacaat gtaattgttg ggaatgtagg
    11221 caacatgtgc tttgccaaaa aaaggcagca tgtgatgctc gcacaaagaa tatacttcaa
    11281 tgtcttttac aataaccatt tgcttatagt cttcgcgaaa acgtgctgac tctaaaattt
    11341 cttttgcatt aaggtgataa ccttgggtga ggaactgcat tgcttttgca acacgttccg
    11401 gtgttttgag aatgccttca cgggaagtat tctctcccag attttgcaat acctctaaat
    11461 aactttgtga caatttattt aacgaatcat gattgtaatg atctatctta cgatagccat
    11521 ccacgtcatc atcatcatcc attatagtgt gtgggtcctt attcattttt cgtttccaaa
    11581 atattcaaca aaattattct ctgtttcgta gagtttaaca gcatgaagca tggctccctg
    11641 actcctgatg gcatcatgca actgattcca tatctgcata gcaagcattt ctgtagaggc
    11701 cattatgcct tgcataaatt tcacatcaag attgatgttt ttatgatcga gcggatcaat
    11761 cacattttct ttaacaatct gactcaatac ctttaaatca atcacaaaac cagtttctgg
    11821 attaggcatg cctttgacag ttataaataa ctcataatta tgtccatgcc agtttgggtt
    11881 ggaacatttg ccaaacactt ctctgttttt ttcctcgctc cactccggct tatacaattt
    11941 atgagctgca ttaaaccgtt cttttctggt aatataaatc attcagtttt tgaatttctg
    12001 caaaggtaaa aaactcaatc aatgccaata taaaaacatt aaatttacac cctgtgaata
    12061 tactgataat tgcagcaaca aaacaagaaa taagtctttt tcagacagaa aaatctgaag
    12121 aaattcactt ttataacagt caagaggtag aaacgctgat atcggggccc ggaattctgg
    12181 ctactgcctt tagtctgact aagaaactaa aagaaaaaaa ttatgacctg ataattaacg
    12241 taggtattgc cggaagttta gatgaaaata ttattcccgg tgatgttgtt caggtcattt
    12301 ctgagcaaat tgcagatagc ggctctgaag atcatgataa ttttctagac ctttttgatt
    12361 taggcctgct taatgctgat gaattccctt tcaaagaaaa atggctttct ccgaaatctt
    12421 ttacgacacc ttcattagaa aaaattaaaa aagtaaaagc tattaccgta aacaaagcac
    12481 atggcaatga aaacagtatt aagcaactga aattaagaaa cacaggacag atagaatcac
    12541 tggaaggtgc tgcatgtttt tatgtttgta tgctgttgaa gacaaatatt attcagctta
    12601 gagccatcag caatattgtt gagcctcgta atcgaagcaa ctggaatatt gaattagcaa
    12661 ttactaattt aaatgcttct ttaaatcaat tactcaatga actacttccg ccagcataaa
    12721 gaaatacaga accccataag gattccggtc gcagtaagaa aaatgcttga tgcaggtgag
    12781 agtgaatttt tagatttcaa aaaagaaata accagcgtgc ataagattgc gaaaacaata
    12841 agttcgtttg caaatcgcaa tggcggaaaa ttgttgattg gtgtaaatga tgataggact
    12901 atttgtggca ttaccacaga agaagaaaaa tttatgattg ttacagctgc aacaaattat
    12961 tgtaatccca ctgttgcagt taaatttaaa gagtggcaat gctctgacaa aattattctg
    13021 gaagtttctg ttccggagag tgaacaaaaa cctgtttttg caaaagacga aaacgaaaag
    13081 tggtgggtat atttgcgaat caatgatcaa accttgttga caagtaaggt gatggtagat
    13141 gtattaagaa agaaaaccga cagcaggtca acactgattg aatattctga gaaagaaaaa
    13201 gctttgatgg aacatttagt tgtccataaa agaataacac tgaaacaata ctgcaaactc
    13261 atcaacattt cgcgttggcg ggcaacaaga attattgtaa acctgattag tgttggcgtt
    13321 ttacaagcca atcattcaga gaaagaagaa ttttatacac ttgcctgatg gaattatttt
    13381 acagcacaaa tattgatgac aatacaattt tgttagatga gattgaaagc acacattgct
    13441 tgcgtgtgct tcgccataaa attggcgatt caattgaagt tactgatgga ataggccacc
    13501 gatattccgg aatcatttct gcagcttcaa aaatctgcaa ggttgaaata acagaaaaac
    13561 attttgagca accttcgaat aagcaattac atattgccat tgcacctcca aagtcaattg
    13621 acaggtttga atggttttta gaaaaagcaa ctgaattggg tattgaaacc attaccccgt
    13681 tacaatgccg ctacagcgaa cgtaaacata taaacgctga gcgatgtggt aaaataatat
    13741 tagctgcaat gaaacaaagt aacagagtct atctgcctgt gcttaatgaa atgatatcgt
    13801 tagagaaatt cataacaatt ccttcaactg caaaaaaact gattgcacat tgtgaagaaa
    13861 gcgaaaagct tactttggaa aaatcaattg cagcagaaca ggtaattgta cttattggcc
    13921 ctgaaggtga ttttgcacca catgaaatta aacttgccat tgacgcagga tatgaaaaca
    13981 tttctcttgg ccaaaaaaga ctacgcacag aaactgcagc cttgcatgtc tgtagtgcat
    14041 ttgcactaaa aaacagaaca ggacaatcgt aaaaaacgac tgtcctgatt caaaatagtt
    14101 tttaagccaa agttgcgctt aaagtaatgt tggtattcag aagttttgaa attggacaat
    14161 tggcttttgc ctcattggca cattcttcaa atttttcttt agtgatgcct gcaacttttg
    14221 cattcagaat aaggtgtgag ttagtgattg caccattatc aagagtaatt tcgcattttg
    14281 tctcgatagc atccggagta aatcctgcag cacctaaaac aaaacttaac ttcatgctaa
    14341 aacagcctgc atgtgctgct gctatcagtt cttccggatt cgtacctact ccatcttcaa
    14401 aacgtgtgtt ataagagtac tgtgattgat ttaaaacagt gctttgtgtt gatactgttc
    14461 ccttaccttc tttacctgag ccattccaaa aggctgttgc gtttctttta attgccataa
    14521 taaattttaa tgtttgagtt atataatttg atatttattc gtattgatta acatttacag
    14581 actgcaaaaa gtttattgaa gactgaatat cggtataaag tattctgtct gtattcataa
    14641 acgaaacctc tttcctgaaa agacttaaaa atgactccaa aggagcagaa gatttcaatg
    14701 gtcttctgaa ttcaaatgcc tgtgcagcat taagcaactc aatagccaat actttctctg
    14761 tattgccgag tactttatac agttttgttg ctgcattagc acccatgctc acatggtctt
    14821 cctgaccatt gctcgacaca atactatcta cagatgcagg catacacaat tgtttgttct
    14881 gacttactat tgatgctgct gtatattgcg gaatcatcat tccggaattc agtccgggat
    14941 ctgcaacaag aaatgaaggc aaaccacgtt gtcctgaaat aagttgatag gttctgcgtt
    15001 cggaaatatt tgccaactca cttaaagcaa tagctaaaaa atcaagaact aacgccaaag
    15061 gctgaccatg aaaatttcct gctgaaataa ctaaatcatc gtcagggaaa acggtaggat
    15121 tgtctgtaac agaatttatt tcattaaaga ttacaaagct cacatggtcc aatgcatctt
    15181 tacttgcacc atgtatttgc ggaatacatc tgaaggaata gggatcttgt acatgcgcct
    15241 tttttctttt aatcatttca ctatctgtca acaactgacg ataacgcaat gccgttgcta
    15301 tctgcccctt atgcgggcgt atctgatgaa tgagatgatg aaacggctca attctgccat
    15361 cgtaagcatc taatgaaagt gctccgataa tatctgcaag caacatcatt cgctgcgact
    15421 gcattactgc ataagtgccg taggcactca taaactgtgt gccgtttaac aatgcaagac
    15481 cttcctttga ctgaagtttt attggctgcc atccaaactg attaagcact tctgctgatt
    15541 taactttctt tgctttaaaa tatacttcac ccataccgat gagtggcagg cacagatgtg
    15601 ccaacggtgc caaatcacct gatgcaccta aagaaccttg cgtataaact actggaaaaa
    15661 tttcgtgatt ataaaaatca atcaagcgct gaactgtctg taactgaaca cctgaattgc
    15721 cataactaag accctgaatt ttcaaaaaca acattaactt tacaatagca gcaggtactt
    15781 cttcgccaca gccacaggca tgtgacatca ccaaattctc ttgtaactta cctaaatctg
    15841 cagtgtcaat actcttatta tacaatgagc caaagcctgt gttaatgcca taaaccggtt
    15901 cggcaagtga gttgacttta tcatcgagat aactgcggca atgtgtaatg cgtttaactg
    15961 catcctcaga aagctcaatt gcaactttgc tatcaatgat ttgttttagt gtattaaaat
    16021 ccagatgctt tgatgatatc tgatgtttca ttattgactt ttattacttt gtatttaaaa
    16081 agctactatt tacttagctt agaaattata tcgtttaaag ttaatttttc ctgctggcct
    16141 gttgccatat cttttattgt aagacttcca ctttgcattt catccttacc gattaaaact
    16201 acgaaaggaa ttttttttga gtcggcatag ctcatttgct ttttcatttt tgcggcatgc
    16261 tccggaaaaa tctctgcatt aattcctgcc tgtcttagct cattcaaaat tttcaaactg
    16321 aactgctctt cttcctgacc aaagttgaca agtaaaacct gcgatgttgt ttcactcaac
    16381 ttaagttgat caaacaaatt catttcaagc attacatcat aaattctgtc aatgccgaaa
    16441 gatattccaa caccactgac attaggtagg ccaaaaattc cggtcaggtc gtcatagcgt
    16501 cctccaccaa gcacactgcc actgaatgtt gaacgtgagt cattaacttt cacttcaaaa
    16561 attgaacctg tgtaataatt aagtccacgt gcaagtgtaa tatctaattc aagattttgc
    16621 agttgctttc caaatgcatt aagatatctt attaccgctt ctgtttcttc aatgcctttt
    16681 aatcctatgg aaacttcagc taaaaatgtt ttcagaaagt tgagctttga ttcaaaatct
    16741 ccgtgtgatt caaaaagtgg ttgcatttta ttaattgcat ctgccgaaag ccctttagtt
    16801 aaaagttctt ccttaacttt ctgttcgcct attttatcga gtttatcaat ggcaacagta
    16861 atatcaatta tcttatcggc atgaccaatt acttcggcaa tgcctgccag aatctttctg
    16921 ttgtttattt ttatggtaac accaatctgc aaactgttga acacttcctg aatgataagg
    16981 agaaattctg cttcgctgat taatgaagtt gaaccaataa catcggcatc gcactgataa
    17041 aattcgcggt aacgaccttt ttgaggacgg tcggcacgcc aaaccggctg catctgaaag
    17101 cgtttaaaag ggaaagcgat ttcattctgg tgcatgacaa cataacgcgc aaacggcaca
    17161 gttaaatcat atcgcaagcc tttctctgac aaatcagaaa ttttaagact atcgcctttt
    17221 gacttggcat cctgaacaac ttcgcctgaa ttcaaaatcc tgaataaaag tttatcacct
    17281 tcttcaccat acttgccttc aagtgttgag agattttcca ttgcaggtgt ttcaatctgc
    17341 acaaacccgt aacgctcata tacctttctg atggtattga aaataaagtt tctacgcacc
    17401 atttctgatg gtgaaaaatc gcgagtgcct ttggggatgg atggtttcat ttggagctgc
    17461 gaattacttc tagttgatgt ttaatagtcc tttacaaact actccttgct gtatataatt
    17521 aacttgaatt gcaagttgaa gttttgttgt ttttaaatgc tgactcataa caatcggttt
    17581 ctcattcgta ttttaatacc aattcgttaa ttgcaatcta gtttttaacc aaactcttat
    17641 acctcactct atgaggagtg acatcaccca atcgtttttt cttattctct tcgtagtcgg
    17701 aatatccgcc ttcgaaataa tacacctgcg aatcgtcttc gaaagcaaga atgtgcgtac
    17761 acacacgatc taaaaaccat cggtcgtggc tgatgattac tgcacaacct gcaaaatttt
    17821 caattgcttc ttctaaagca cgcaatgtat taacgtcaat gtcgttggta ggctcatcaa
    17881 gtaacaacac gttggcttca ctacgtaaag tcaatgctaa atgcaatctg ttgcgctcac
    17941 caccggaaag cactccgcat ttttttccct gatcagcacc actgaaatta aatcgtgcaa
    18001 cataggcacg tgagttcaaa agtttaccac caagctcaat ggattcattg cctccggaaa
    18061 ttacttccca tactgttttt tcaggatcaa tttctttatg cgtttgatct gcataaccaa
    18121 tacgaaccgt actgccaatt ttaaattctc ctgaatccgg cttctcaagt cccattatca
    18181 tacggaaaat tgttgtcttg ccggctccgt taggcccaat aatgcctaca ataccgtttg
    18241 gtggtaattt aaaattgaga tcgtcataca acaccttatt accataggct tttgaaactt
    18301 taatggcttc aattacttca ttacccagac gaggtccgtt aggaataaat atttcgagtt
    18361 tctcttcttt ttgctttaca tcttcgctca gcagattttc gtaagcattc aaacgtgcct
    18421 taccttttga ctgccttgcc ttgggcgaca tgcgcaccca ctctaactca cgttgcaaag
    18481 ttttctgacg tttgctttcc tgtttttctt cctgtgccag acggtttgat ttctgctcta
    18541 accagcttga gtaatttcct ttccatggaa tgccttcacc acggtcgagt tcaagaatcc
    18601 atcctgcaac attatctaag aaataacggt cgtgtgttac ggcaatgact gttcctgcgt
    18661 attgctttaa atgctgttcc agccaatcaa cagactctgc atccaagtgg tttgtaggct
    18721 cgtcaagaag caatacatcg ggctgttgca ataacagacg acataatgca agtcttctgc
    18781 gttcgccacc ggaaagaaca tttaccggag catcggcctc aggacaacga agtgcatcca
    18841 tagcaacttc aattttgttg tcgagattcc atgcatctgt agcgtcaatt ttttcttgta
    18901 gtgcagcctg acgattgaga agcttgtcca tcttatcggc atcggaataa tattcttcct
    18961 cacccatttt gttgtttaca tcatcgtact ctttgagcaa gtcaacaatg ggttgcacac
    19021 cttccatgac aatttctttt gccgttttac tctcatctaa cttaggctcc tgctccagat
    19081 aaccaacagt gtagccgggt gaaaacacta cctcaccggt aaatgatttg tcaattccgg
    19141 caataatttt cattaatgtt gatttacctg caccattcaa accgaggatg ccgatttttg
    19201 caccatagta gaacgagagg taaatatctt ttaatatttg tcgttgtggc ggaacagttt
    19261 tactaacgcc caccatcgag aaaataatct ttttatcgtc actcattgca gcttttttta
    19321 attggtgtgc aaaggtaggc aaaatgaagg tttggattgc ctggaaggtt ctaatgtttg
    19381 aaatattgcc atattatcct ccgatgaaaa atcgtggaga cactgtgagg gcaaaaatga
    19441 attatccaaa cttgaaaagt taaagatata aatttaaaat attgctgaaa aaaatatcat
    19501 tctattgtga ctaaagtcac attaagaata acaaagacgt cttaatttta ctcaatcaaa
    19561 caaaaaaaat atgaaacact ttacaacatt ccttattcta ttgcttacaa ccatcgtaaa
    19621 attacatggt caggtaatct acaactatgc tggcgactta aacggtcaac ctatgtatgt
    19681 tgatgcaaac gtcagtggaa cgcccctcta tactgccggt attttaggat accgatgtga
    19741 ttctggatat accgtaagga caaatggcaa tgatccgttt tttcacttcc aaaaaggtcc
    19801 ggtagatgaa tttgatttcg atgcaaatga ccgtattgat tttacattaa caccccaatc
    19861 aggttatcaa atgaatataa cgggaatgac agttgtagcc ggaagactta catatggccc
    19921 tgaacatcta aagtttggtt taaacattaa cggtacaggt ttaatagaag gtgttcaaat
    19981 ggacaccttg cctttagagt gtgatggcga tcctttaaat ggaattccca atgctgttcc
    20041 ttatactcaa gtatgggata ttaccgattt taattctctt tcacctgtta ctttcacaat
    20101 aggatgcttt ggcggaataa ataatgtggc aataatttat cagattatta ttcatggcac
    20161 tgtaacacct gcacaaacta ttcctcaaat ttcaagctac tcccatcccg gtggatacat
    20221 gtattgcgat gatgaatcta taacactatc agtggtagcc tcagggactc cttctccgtc
    20281 ttatcaatgc caagaccaat acggtaatgc gattttggga gcaacacagt caacgcttac
    20341 tttgcctcaa cctttaccac aacacagtta ttatcgtgta aaagtttata acacttttgg
    20401 agaaacatat tctgcctatt cacatattta aggttacgtt aatgatatca acgctggaac
    20461 tgaccagcaa ttatgtgcgg gagatcaggc tacactaaat ggccagggat ctggcttttc
    20521 aaattggagt acatcaggtg atggaagttt tcttgatcca tatatgttaa gcacaagtta
    20581 taatcctggt cctggtgatt tattaacagg caatgtacaa ttaaatcttg aaggatatgt
    20641 tctcaataat tttggatatg aagtatgctg gaggcaagac tatttaaatc ttacaatagg
    20701 tattccccca acagcaaatg ccggaaatga tcaggtaatt ttgcaatcaa atactacctt
    20761 gactgcaaat gacccatcac ccggaaatgg cttatggaca gtgatttcag gaactgcaac
    20821 ttttactgat gctaccctat ataatacaaa tgtcagcaat ttaagcaatg gcaataattt
    20881 attacgctgg acaattacaa tgggtacatg ttctagttat gatgaaatga ccgttacagt
    20941 agcaacaaaa ccggtctctg cttttctttc gggagacaca actatttgca atggaggact
    21001 aactcaactt aaaattgatt ttacaggaag tgggcctttt gactacagtt atactgatgg
    21061 agttagtgtt tttggacctt tcacagccgt tgctaaccct gaaataatat gggttaatcc
    21121 tgcttctacc accaactatc aattggtatc agttagttca ggaggtacaa gtggcacagt
    21181 ttcaggatca gccaatgttc aaattttcac tgctcctcca tcatctaatg tgacattacc
    21241 atttaacgga atgccaactc atatatgtaa tggaacaact gcaaccttat ccatcgcttc
    21301 tgttgcaggt gctacctact atatatggga tgcaccggcc ggttcatact ttgatggaaa
    21361 tccattaaat acctccccct actctagtac cttaccttct gtacaaataa catttgcagc
    21421 tccatcaggt tctttctaca caataggtgt tcaagcagca aatccctgcg gaactacttt
    21481 acgtaaaatc gaaaaagtca gaggattaat atcaacgcct actgcaataa atggtttaac
    21541 aatagcatgc gctaatacca atggaagcta cagcataaat gccgtaaccg gtgccacatc
    21601 ataccaatgg tcaatttcag gtgatgcaac agtaagcggt aatggcacat ctgtatccgt
    21661 ttcattcggg cctacttgga ctagtggaac tttatgtgtt gcagctcaaa caggttgtta
    21721 cactacagtt tcaaaatgta taaatattga aaatattgta actaaagtat atacgccaac
    21781 aggaactttt gctgcatgtc ctaactcagt tttaacatat agtatcccgt cacaacccgg
    21841 agttacaaca tataattggg cattacctaa tggagcaaac ggttattcta caacaaacag
    21901 tattatagct accttcggac ctaactttat taatagcggt aatatttgtg tgtcagcaac
    21961 aactatttgt ggaacaacta caccaccaaa atgtaaaacc attaaaatag gattacctga
    22021 aagacctact tctattacag gatctactac aggattatgc aatacttcac aattatacac
    22081 atgtccaata ataagcggtg caacatatag ttggactgct ccggcaggta ctgtaataac
    22141 cggaaatgga aacaatgctg ttactattga ttttggaacc ttcactacag gatcgttgtg
    22201 tgtttcagca acaaacagtt gtggtacagg ctctccgcga tgtataactg taaagggtgc
    22261 tcctgctaaa cctggtgtaa tttcaagcct gccaaataca tggtgtgctc ccgaagccgg
    22321 tgttcagttt aatagcaaca tcagtaatct tacaggtaac tacaacctaa aatggcaaat
    22381 agctccttca acaaatgcta ctatacaagg tccaaataat actaatcaaa tgtcagctga
    22441 ctggcttgta tccggaacag catccgtgca gctaatggca tataatgcat gcggtagtgc
    22501 tacaataact catgctttaa acattcctgc atgtaaactt tctgcttcaa attttaacga
    22561 cctgcaagaa ggtattttaa tataccctaa tcctgcaagt gatttcatta caataaaatt
    22621 acctgaagat ttaagttcag atttgaaagt agaattatat acttccgaag gaagtctcgt
    22681 tagtagaagt accattaatt ctactcaatt actttctctt ccaccatcag caggattata
    22741 tttcatcaga gtaatatctg caaaacaatc acaaacctta aaattggttg ttgaataaat
    22801 ttttatttaa cgatgaattc ttaacataat ttattcatcg gcactgtatg gcttttcaaa
    22861 caagagcagc gaattagcaa aagttaattc gctgctcttg tttaatacaa acatcctcta
    22921 ttccaatttc attatcgctg ttgatgacgt tgtttgaata aaaaataatc tctgacgtta
    22981 tgttacgagc ggaaagttta gttttgccgg ttctgacaga atggggaata tgaccaataa
    23041 gaaaaagcgc ggagttttcc gcatgtttgt tttgctgatg tgggtgatgg cattgttgcc
    23101 actggcgata atagtgatgg tattgggtgc tgtgaacatg ggtaagtttg gtgagttgcc
    23161 aacatttgaa gaactggaga acccaaacag cagtcttgct tcggaggtta tttcaagcga
    23221 caatgttttg ttgggtaaat attatattca gaacagatcg aacattcatt ataaagaact
    23281 tgctgaaaac actgttgagg cgctgaaggc tactgaagat attcgctttg aggaacacag
    23341 tggtgttgac atgcgcggat tgggaagggt gttttttaag acggtgattt tgcagcagcg
    23401 ctcttcggga ggtggcagca caattacaca acagttagcc aagaatttgt ttccacgtga
    23461 gaatgaaagt ggtcttcggt tagcacttcg caaggttaag gagtggatta ttgcagtgcg
    23521 gctggagcgc aactacacca agcaggagat tatggctatg tatctgaata cagttgagtt
    23581 tgggcataac agctatggaa taaaatcggc agcaaagact ttttttaata aaatgccatc
    23641 gcagttaaat gtagaagaat cggccatgct ggtgggtttg ctgcaggcac ctacacgtta
    23701 cagtccggtg cgcaacccaa agaatgcatt ggccagacgc aacaccgtgt tgagtcagat
    23761 ggagaagtat aaatttttga gtgaggcagc atatgactca atagtaaagt tgccgataaa
    23821 attaaatttc agggcagagg atcataatga cggactggca ccctatttca gagagagctt
    23881 gcgattggaa ctggtgaagt ggtgtaagga gcataaaaaa tctgatggca gcagttacaa
    23941 cttgtatcgt gatggtttgc gcatttacac tacgatagat tcgcgattgc aggagtatgc
    24001 cgaagcaggg atgaaggagc atttgacaga attgcaaaaa actttttatc agcattggaa
    24061 aggccgcata ccctgggcac agcatccgga ggtgattgaa gagggaataa agaaaagcga
    24121 ccgctatata tcactgaagg ctgaaggcat gagtgaacag gagataaaag aaaattttgc
    24181 cactaaaata cctatgactg ttttttcgtg gaagggcgac atagacacaa tgatgagtcc
    24241 gcttgactca ctgaaatatt acaaaatgtt tttgcaagcc ggtgtgatga gtattgagcc
    24301 gcaaacaggc tatgtgcgtg catgggttgg cggaaacaat tatcgttact tcaagtacga
    24361 ccacgtaaag gctggacgca gacaggtggg atcaacattt aaaccatttt tatatacact
    24421 tgccatgcag gaaggctatt caccttgttt taaagttccg aatgtgcgtg taaccattcc
    24481 aatacccggg cagccagact ggagcccttc caatagtgat ggaaaatatg gtggcatgct
    24541 gacactgaaa gaggctcttg cagagtctgt gaatagtgta tcagcctatc tgatgaaaca
    24601 gtttggcccg aaaccgatga tagagattgc caggaaaatg ggtattacct cgcccattga
    24661 cgaagtgcct tctatttgtt tgggaacacc tgatgtttcg ctgtttgaga tgtgtggcgc
    24721 atacggcacc tttgccaata aaggtgtgtg gacagaaccc atttacatta cccgtattga
    24781 agataaaaac ggaatggtgc tgcaagagtt tgtgccacgc aaggttgaag ccattagtga
    24841 agagacagct tatctgatgc tgaatctgat gcaaggtgtt gtacagggag gaaccggtgc
    24901 aaggctgcgt ttccgctttg gactcaccaa tccaattgcc ggtaaaacag gtacaactca
    24961 aaatcagagt gatgggtggt ttatgggcat tacaccaaca ttggttacag gagtttgggt
    25021 aggttgcgaa gaccgctcag tgcatttcag aactaccagt cttggtcagg gtgcaaatac
    25081 agcattgcct atatgggcat tgtatatgaa aaaggcttat gccgataaag atctgaattt
    25141 gtttaacggt gattttgaaa aaccggaaca tccgctgagt atcgaaacag actgcagcaa
    25201 gtatgagcaa gacagaaaag atcatcagca taacagcggt tttggaaacg acctttagca
    25261 gaatgaaacg ctaaatattt ttgtctgccc gaataattta attttgcatt ttcactttca
    25321 ttaaatataa gaatgaaaat atcgctcgac tggttgaagg actatattaa gttgcaacag
    25381 acacctgaag aaattgaata tttactgaca agtagcggac ttgaagtgga gtcgttggat
    25441 gaaaccgaca gtattaaagg cggactgcgt ggtttggttg ttggtgaagt acttgaaaaa
    25501 gaaaaacacc ctgatgccga caggctgagt gttacgaaag ttaatgtagg caaagctatg
    25561 ccactacaga ttgtttgtgg tgcagctaat gttgctaaag gtcagaaagt attggtggct
    25621 ttgaacggag cacgactgta tccaaccttt ggcgaaccat tcgacataaa aaattctaaa
    25681 atacgcggtc aggcatcaga ggggatgatt tgttcagagg atgaaatagg tcttggaaat
    25741 tcgcatgagg gtataatggt tttagatgcc aatgccgtac ccggtactcc tgctgcggaa
    25801 tggtttaaaa ttaaacagga tcatattttt gaaataggac tgacgcctaa ccgcattgat
    25861 gcagcttctc atggtggtgt agcacgcgat cttgctgctg taattaatgc aagagcaaag
    25921 gcagaaaaca ataattcaat aacagcacaa ttcagtatgc ctgatgtgag tgcttttaaa
    25981 cctgaaagca acgcactgca catagacgta gttgtggata aaaacacagg ttgcggaaga
    26041 tattgtgggg ttgccataag cggtgtaaca gtaggcgaat cacctgagtg gctgaaaagc
    26101 agactgcagt ctataggagt taaatgcatt aacaacattg ttgatgtaac caactttgtg
    26161 atgtttgaaa ccggacaacc gctgcatgcc tttgatgcag ccagggtgaa aggaaaaatt
    26221 attgttcaga agacaggtaa ggcatctaca tttactacac tcgaccacat taaaagaaaa
    26281 ctgacaggtg atgaagtgat gatatgtaac gccatagagc ctttgtgtat tgccggtgtg
    26341 atgggtggaa ccgactccgg tgtgtctgct tctacaacac aaatattttt agagagcgcg
    26401 tggtttaatg ctgccgaagt tcgaaaggct gcaaaacatc atggtttaaa gacagaatca
    26461 tcatttcgtt ttgaacgtgg taccgaccct aatggaacat tgtacgcatt gaagcgtgca
    26521 gccttactta ttaaagaagt tgccggaggt aaaattgcat catctgttgt tgaccagtat
    26581 cctcaagttg ttgaaccgca aatcatagat attaattttg aaaaaataaa caaactgtca
    26641 ggaattaatt tcagtaatga catgttgctt tctgtgttgg aatattgtgg ttttagcatt
    26701 aaaggcagca gtgcaaatgg cgcaactatt tctataccga catataaaac cgatgttaca
    26761 cgacaggctg atgttgttga agaaatttta cgcacttatg gctaccatca cattccattg
    26821 aatgaaaaaa caggtatgtc attttcaagc catacccctg acagaaaacc tgcaatgcaa
    26881 tttaaaattt cacatcatct gagctctgta ggattttttg aaatgataaa caattctttg
    26941 gtatcatctt cacattcggc tgcagtagat gaaactataa aagttttaaa cccgttaagc
    27001 aaagaattag acatattgcg tccggacatg attgcctcca cgcttaacac catttgttac
    27061 aatataaaca gaaaaaacca taatttaaaa ttgtttgaat ttggcaagac ctatcttcag
    27121 catgatggaa aacttactga aactaacaat ttagttattg ccatgacagg catggtgaat
    27181 gagcctaact ggcataaatc tgaaaaagaa tcgtcattat tttatcttaa atcagttgca
    27241 gaaaaagcta ttgagttagt gatgccgcgc ataagttctc atttaagttg ggtgcaggat
    27301 agccatacta tgttgtcatc ttacacaaac atcatgcaac ggcagtcagc atttggttat
    27361 attggatcag ttaagaaaaa tgtgttgaaa caattcgata tttcgcaacc ggtttttatt
    27421 gctgtactga atatggatgt tttactcgaa aaaagtttgt cacataaaac agctatcaaa
    27481 cttccacaac catttccgga ggtaaaacgc gacttgtcac taatgctcga tcagacaata
    27541 aacttttccg acctgagaga tttagcattg aaacaagaaa ggaagttgtt gaggcaggta
    27601 gatatttttg atgtgtatga gggtgaaaag attgaagcag gaaagaaatc gtatgcattg
    27661 agttttactt tggctgatga tgaggctacc ttaactgata agcaaattga atcagtaatg
    27721 caaaagattg aaaaggcatt tgaaaaaaat ctgggagcag taatcagaaa ataatgtcgc
    27781 ttgatttatt ttatcgtagc agtggcaatg gtcagccact aattatcttg catggtctat
    27841 ttggattgaa tgataactgg caatcttttt caaaacaaat aactgaaaat gatattgccg
    27901 tatatgctgt tgatttaaga aatcatggaa gttcgccaca tgaccctgtg tttgattacg
    27961 attcaatggc tgatgatatt tttgcattta tccgtacaca tgatttaaaa gatataaaca
    28021 tcatgggaca ttctcttggt ggcaaagtag ctatgaaatt tgccttggaa catcaggaga
    28081 ttttaaagtc attaattgtt gttgatattg cgccacgata ttacgcacct catcatcagc
    28141 agataataaa agcacttcag gctgttgatc tcgatacggt taatacccga aaagaagtag
    28201 agcatattct gtcgggctac attgatgatg ccggagtaaa aatgttttta ctcaaaaatt
    28261 tatattggga aggcgaccgt ttgcaatggc gttttaatct gaaagtaatt gcagaaaaaa
    28321 ttgaatcggt aggtcaaaga attacatcaa cagaaaaatt tgaaggaaaa accttgttta
    28381 tcaaaggtga aaaatcaaaa tatataaccg atgatgataa gcaggaaatt ttaagtttat
    28441 ttcctcatgc agaaataaaa acctgtgctc atgccggaca ttgggttcat gccgataacc
    28501 cggaatgtat gcttacaaca gtgcgtgatt ttttaagtca ataatcagta caaacatcat
    28561 cagtatttca gtattttagc gcatcatttc ggagcgtaaa taatggacta tcaattcagg
    28621 gaaacagaaa aaaaatggca gcaatattgg gctgcaaaca aaacttttaa ggttgaaaat
    28681 aaatctgaca agccaaagtt ttatgtcttg gatatgttcc catacccttc cggtgccggg
    28741 ttgcatgtag gccatccttt aggttatatt gcctcagata tttattcacg ctttaaaaga
    28801 ctgaatggct ttaatgtttt gcatccaatg ggatatgatt cttttggatt acctgcagaa
    28861 caatatgcaa ttcaaactgg tcagcaccct gcaattacca cagaagcaaa catcaatcgc
    28921 tatcgtgaac agttagataa gatggggttt tcatttgact gggacagaga agtacgcacc
    28981 tctgaccccg cctactacaa atggacacaa tggatttttt taaaactttt taatgcatgg
    29041 tataacaata aaagaaactg tgctgaatca attgacacac tgatttctga atttgaaaaa
    29101 aacggcaaca atgatattcg tgctgttcat gatgagatag agaatttcac tgcagcacaa
    29161 tggaaaagct ttgatgaagc tgaaaaaagc cgtattctca tggcctatcg ccttgcctac
    29221 ttaggcgaag cgtatgtaaa ctggtgtcct gctttaggca ccgtattggc caatgacgaa
    29281 gtaaaggacg gacttagtga acgtggtggt catcccgtag aacgcaaact gatgaagcaa
    29341 tggagcatgc gcattacagc ctatgctgaa aggttgttgc aaggattaga agatattgat
    29401 tggagcgatt ctctgaaaga agcacagcgc aactggatag gaaaatcaga aggaacaacc
    29461 atcaaatttt ctttgcagga ttctgctgaa tttatagaag tgtttacaac acgccccgat
    29521 acattgtttg gtgtttcgtt tgtaacctta gcaccggaac atgagttggt tgataaaata
    29581 acatcttcat cacaacgtgc agaagtgcag gattatgtta ctaaaacaaa aaacagaagt
    29641 gagcgggaac gtcaggctga ggtaacacga atcagcggag tgtttacagg cagctatgtc
    29701 attcatcctt ttaccgggaa taaaattcct gtgtggattg gtgactatgt acttgccggt
    29761 tatggcactg gcgcagtgat ggctgttcca gcacatgatg aacgtgactt tcgttttgca
    29821 acacactttg gacttgaaag gccacaagtg atagaacccc atataacgca caacttcgcc
    29881 aatgcctcat gggacaataa agaaggaata ttgattaact ccgatttttt aaatggcctg
    29941 gatgtaaaaa ctgccattag ccaatgcata acagaaattg aaaaacaaaa tctgggtaaa
    30001 ggaaaaacaa actatagact tcgtgatgct gtctttggcc gtcagcgtta ttggggcgaa
    30061 cctattccaa tttattataa agaaaatatt ccggtagcaa ttcctgaaaa cgagttgcct
    30121 ttagtactac ctgaagtaga taaattttta ccgacagaaa ccggtgaacc gcctttggca
    30181 agagctaaaa actggaaata caaaaatcag tttcctttag aaactacaac catgccgggt
    30241 tgggcaggca gcagttggta ttttttacgt tatatggatg caatcaacag tacttccttt
    30301 gcatctaaag atgcgctgaa ctattggaca aatgttgatc tctatattgg cggcagcgaa
    30361 catgcaacag gtcacttgct gtatgtgcgt ttctggacta aggcattaaa agatttagga
    30421 tacattaaca ttgacgagcc tgcacaaaaa ttaatcaatc agggaatgat acaaggcaca
    30481 tcatgtatgg tgcatcgtga tgttgcaggt aataaaatta tctctgccgg attaaaatca
    30541 aattacaata ctacactcat tcacattgat gttaatttgg tggacaatac agaagttgat
    30601 attgaaggct taaataaatg gagagatgat tttgcaacag ctgagtatgt tacagaaaac
    30661 gggaaactga tttgcgaaac tgccgtagaa aaaatgagta aacgttggta taacgttgta
    30721 acacctgact cgatttgtga aaaatacggt gcagattgct tacgaatgta tgaaatgttt
    30781 ctggggcctc ttgaacaaag caaaccatgg aatacgaatg gcattgcagg tgtttctaat
    30841 tttatcagaa agctttggaa attgtatcac aacaatggca aatttgaagt aagtaatgaa
    30901 gcagcaacaa aagctgaatt aaaaacactg cataaaacaa taaaaaaagt acaggaggat
    30961 gttgagaagt tttcgttcaa tacttctgtc agcacattta tgatttgtgt gaatgaacta
    31021 acggaacata aatgcaataa gagagaagta cttgagcctt tagcaataat actctctcct
    31081 tatgctccac acattgcaga agaactatgg catcttcttg gccacaatac atctgtttct
    31141 tttgcaactt ttccggaatg gaatgaaact tacttaatag aaaacgaatt tgattatccg
    31201 gtttctgtaa atggcaaaac cagatttaaa attctattac ctttaaatat tggcaaagag
    31261 gaaatagaaa gacatgtgat ggcctctgat gagatgcaga aatggaccaa tggcaatgca
    31321 cctaagaaag ttattgttgt tccgggaaga attgttaatg tggtgattta gcttcttgag
    31381 caacaggtta atttttaatg ataaagataa caactcgtga acagcaattt gacaggtttc
    31441 ttgaaagtgt ctagttaaag aataagaaaa taatttgtag atataaaacc atcgagagct
    31501 acaattgaat ggtaataaaa tcatttgatg gattggtggt tattaaagcc tcctctagct
    31561 gattataaaa ttttgaagca aaagtttaaa ttcagaaatg tttaatgcat gagttgttaa
    31621 aacacttagg taaatattat cctactgttg gcgatacaca ttatctaaaa tttgtttagt
    31681 taaatttgat agcctgaaac agaatagttt ttaatgttat tttcttgaat tagtgtgatt
    31741 gcaagaatat gctcatccag cataaagaac tatacactat cgcaattaac ttgtttttcc
    31801 aaggttatta gatttttagt tctttcattt ttttaacatt gccaacaagc cagacaagat
    31861 catcagtttg aaatatcgtt gttgagtcag gatttaaaat tcgttctccg tttctttcta
    31921 ttcctgctac aagacctaat gctttttctc tgatgcctga acttcgaatt gactttccgt
    31981 tgagccaatg atgttctgta attgccaatt gaaaaacacc aatatcatct agtgaggtag
    32041 gttctgcgtc ttcttcaatt ggattttcaa aaaccgtttt gaacacttgt atctgttcat
    32101 ctgtagtcaa aacacctact tcgtcaaaag gcaacaacaa ttgattacga ccgggtatat
    32161 gatatatttt atcccctctt ctaatataaa ctatgttgat tccatatttt tctctccagg
    32221 caagttgcca taattctttg ccaataaatg ctgcattggc aggaacttcc agttgtacaa
    32281 tatgtgcatt atacggtgat aaaaaacttt gtacttcata gtttttcctg tttaagcttg
    32341 cagaagtaga atggtactca acactttccc tttcgctgat attagtaata aaccttcctt
    32401 caattctttt ataaaatttt tgtaggtgca tagcaaatac tctgaaaata attagcgtta
    32461 ttggcagaac caccaccaaa gcgataattg gtttaaaaaa gtacaatgcc catcctgtta
    32521 ttaatgcaac acccattgca acccgtgtca gctcaattat taataatgga cctgtgcgaa
    32581 attctttttt acttaaccat aaatttttat agtatggatg atttggcttt ctggccacaa
    32641 tggcccaaat aaatggagca ccagcagcca atgatattgc cgggacagcg atagctttaa
    32701 ctatttcgtt tacctgaatg gtattgaaat aaggaattaa gatacggtct aggagaagtc
    32761 ccaatgccaa aagaataatt ccattgatta ttacaatcct actataagcc gcaagtaagc
    32821 tttgccatcc ggttgcagat tgaatttttt gggttccaga tgaaaatctg tttagatact
    32881 ctacccatct tgatggcagc agtttttcaa tttgcatata aataggttct gtatatttta
    32941 tcaggtaagg tgtggtaaat gtggtaattg ctgaagctcc aacagccacg gggaagagaa
    33001 aatcagaagt aacttttaaa gatgctccca aagcagcaac aataaatgca aactctccaa
    33061 tttgagccat actaaaacca acttgtagcg attgctttaa tggctgtccg gatattaatg
    33121 caccaataga agtactgatt atctttccga aaagggtaag tagtgtcact gcgacaacag
    33181 cccatttatg atctatgata gcttgggggt ctatcatcat tccgactgat acaaaaaaga
    33241 ctgcgccaaa taaatctttc accggtttgg ttaagtgttc tactttttca gcagaggttg
    33301 tttctgccag aatgcttccc ataataaatg ctcctaactc agcagaaaag cctgcttcta
    33361 ctgcaagtac aaccattcct aaacaaaggc caatagaaag aattaacaaa ttctcatcat
    33421 ccagccattt tttggcattt ttcaaaaggg ttgggattag aaaaattccc atgataaacc
    33481 aaagcgaaag aaagaacaat agtttgagga caataaaaac catttctcct ccttcaattt
    33541 gctgagttac tgctatggtt gaaagtaata ccatcagtaa gattacaaca atgtcttcaa
    33601 ctatcaatac gccaaaaaca acacgggcaa attgtttagt ttttacgcca agttcatcaa
    33661 atgctttgat gatgatagtt gtggaggaac tagccaacat tcctccaaga aacaaactgt
    33721 ctatcactga ccacttcaac catcttccta aaagaaaacc tgtaattgta ataaatgcta
    33781 tttcaacaaa tgcagttatg gatgcagagc caccgactcg catcaacttt ttaaaactaa
    33841 actcaagacc taatccgaat aacaggaata ttacaccaag ttctgctaca acatcaatgc
    33901 tttcaatatc aataaccgtt ggaaaaagtt tgaaatgtgg tccaactaga aatccagcaa
    33961 tgatatatcc aagtacaagc ggttgtttaa ttcgtttaaa aagtaatatt gttgctgacc
    34021 ctgctaacaa tactaacgct aaatctacaa tcaaatgtgg aaggtggttc attgaaaaaa
    34081 tttaaaataa ttgtcaatac tatttaaact gaattaacgg atgtggcaga atatcaaaat
    34141 tatttctttc attcattctt acaggaccca tatgatcaat ataaatgaat ggcccaacca
    34201 tgcgctttag ccggaatggc aataaacggt caaccataaa ttctccaata tctgctgctc
    34261 tttcctcaat tattaaactg ttgtttgaca taatctaatc agaatttatt acatcctttt
    34321 ttattaatta aagataaata tccgatgtat tagttgtcaa atatataaaa ataccattga
    34381 attcttcttt aggtgtaatg aatgatagca ttcaaatctt attatacata acctcaatta
    34441 ttaaaggaaa aacgctctga tagaaatagc ctaaagatta ttgatagaat aaattttgtg
    34501 tttaaacaaa tgaaaccgat tttatcattt cagactaaaa aatatactgc aaggtgcaat
    34561 aaagaacttt agaaaaataa atgttattct ataatgacag gtattaaatt tttctttttt
    34621 agttgcttaa agcccaagaa aaaaatcaag acaaatagta ttccgatgag tgcttctgca
    34681 cctagcataa atttacaacc aatttatcat gtgtccatcc attgaatgtt ttcatgtaaa
    34741 caacaggcag gttaccaagt gatgacatta atgaaaactt tgtggcagta tgtttttttt
    34801 ccgataacaa ataaaattac ggctgtatat gcagcataca tgagtccggt acaaaaccat
    34861 gactaatacc cataggcatc aatacaaaaa aatatataat ggctttgtgt tatttgtttg
    34921 cattctaact ataactttaa gttagtgtac ttgataaact tattctgttt ttattaatgc
    34981 aaagttgttt tttatcattc ctttaatcct ttattagata atgattacta atttaaattg
    35041 caatactgca atataaaaaa agtagttatt ttttggtgat gatgattaaa aaaatcactt
    35101 cgattaaact acaaataaat aaaactgtaa atgcaaatta ttatttacta ataataaaat
    35161 aatcagaaat tattttgttg ctttcgtgta aggcacgatc tgcaacggaa acctcaacac
    35221 gaaatgtatc tgcaacacca ttagttgtga agcttaaaaa attttgtaca tcaaccttaa
    35281 tgtcaccttt aatattttta tttcgctgct tgggtgtaag tattggaatg cggtagtaat
    35341 aaggaatatt aaagttaacg ggtgtccaat tgccgttgat aagttgctgg tagttacaaa
    35401 aataattgtg ataaaaatga ctgtttttat cgaatggtgg aagtgtgtcg gcatcactca
    35461 gtccaatatc gccatcacca tcagtaaaag ttgtaattaa ataaaagctg ttttttccat
    35521 aaaaatcaac agccttgaat gttaatacag gttctacagg atatttttct tctttgcggc
    35581 atgaggtcaa aaaaacaatc aatataaatg caagaaccgt tttcatttct actaaatgct
    35641 aaaaccaacc cacatgatca gaaatcattc ctttacttat tagaatgaca actacaataa
    35701 tcataatcag aaaggctaca ccaaaagtta ttgttaaaac tttatacata ccggcattac
    35761 gtttcagttg tgccagttta aacaaattga acactgtctt ttctgcacga tagaacgcac
    35821 tctccccctt cacaatatag tcgaatgcac catatttcat ggtgtcaaca gcaacttcaa
    35881 ttttatcctg accggaaagc atgacaactt ctgttgtagg gtattttgct tttatttctc
    35941 ttaaaacatc tattccatcc atggcattag cgattttaga atcgaggttg taatcaagaa
    36001 atacaatatc aggaactaat tgaccggaag ccaatgcctc ttcacctgtg gcaaaagtgt
    36061 ggattgtaaa gtttgacatt gccgacagat gatctttaag catattctgc atcatcgggt
    36121 catcatcaat gataaatatt gttgaggttt ttgacatatt atgactttag tattggcatg
    36181 acaagaatac aaattaattt tgagctataa acgcctttag ttcatctttt gataaagtta
    36241 tcatctcctt tagttgtgta atatcatgag ccatttcctc tgtgacactc tcattagaag
    36301 cgctatcttc aatttttttt aacagttgtt gtgcacgttc catacccata taagctgcaa
    36361 tagattttaa ggtatgcgaa gcggtcttca attttttata atctttatca tgaaatgatt
    36421 tttctattac aggcatttct tcatcggcac tttgtatata catcatgaca tactttttaa
    36481 ctctgctcat gtcgccttta ctgaatttct gtataaaatc gaggttggta actttttcca
    36541 ttgtgcaaaa atagttattc aattaaaaga ttgcatcccc tcacttctgc attttcgaac
    36601 ctaccgagaa tttcgaaagt cccgtcatca taaaccctac caatgtcttg tgtcgaaata
    36661 aagcaacaac tgttaatatt tgccaaatca atgatattga ttccaccctg tccaggaata
    36721 ctttcagaca gtgggtcgtt gctatcgcgt acaaaaactt tcatccatgg tgggcaatga
    36781 aaaataccgt ttccactact gtatgcctgc gagagtagtt ctgtcattcc atattctgaa
    36841 tgaattacat tcagaagaaa tgcttctttt aagatagcat gcagttcctg cctgatcatt
    36901 tcttttcttc tgcctttcat accacctgtt tccatgatga ctgtattctt aagtggcatt
    36961 ggatatgcat gcgcaaagtc aagcagggca aaacttacac ctataagtaa tgtcttaatt
    37021 cctctttgtt cgtttaccgt aatggtatct gctaattttc tgaaatcatt cagataaaat
    37081 ccactgttct tcttccctgt tttctcaaca aggtgttgac acatatacac caacgaagag
    37141 ccttcgcgct ccagataaga tggtagtaaa gctaatatac accattggtc agtacttgta
    37201 taaaaatgat taaaagcttg cgtaaaagcc ttttcgtata atgatatatc gctgacgtaa
    37261 tgtagtgatg gctctgtacc ggtagtgcgg ctgcttgaaa aaactattgc acttgaatca
    37321 atatttcccg ttaccacttt cttcgtctta aaaaaactta ttggcaaaaa gggtatttta
    37381 ctaatgctgt caattttcag tggattgatg tttaataatt tcagatagtt gtgatatact
    37441 gcgttattct ccgcctgaaa cctgaaactt tcaagtgcaa cactttcaaa atcaaatggt
    37501 gtatcaaaaa ctgaagtcaa ggcagaataa ataaaattag ttatctacat taaagtgaag
    37561 ccccttattt tcagtccttg ccattgcagc tttaataatc aggtatgata aattaatgag
    37621 gttgcgtaac tcgcaaatac gaggagaaat ttcccatctg ttatacaatt cttcagtctc
    37681 ttcatacaag agtcgtgtac gttcaagcgc ccgtttcagt cgcatatcac ttctgaaaat
    37741 accaacatag ctgctcatta tttcctgcaa ctctttccgg ctttgtgtaa taagaaccat
    37801 ttctgcaact tcgcgtggcg acacagcatt ccactccggg atgtgttcgt tgtaatgaat
    37861 aaactccaaa cgctccgagg catgtacata agcacgatga gcaaacacaa cagcttcgag
    37921 caaagaattt gatgccagac gatttcctcc atgcagacct gttgacgaac attctccaat
    37981 tgcatacaga tgttgtatag acgattgtcc atgttcgtca acttttattc ctccacataa
    38041 ataatgttgt gcaggtgcca caggaatgca gtcttttgca acatcaatgc ctaaagacat
    38101 acaacggttg taaatatttg gaaaatgctt tttaaactct attatattta acttagtgca
    38161 atcgagccag acataatcgt caccactttt tttcatttcg ctatcaatag cccgagcaac
    38221 aatatcgcgc ggggcaagcg atccacgctc atcatagcgt tgcataaatt ctttgccgtc
    38281 aatagtgcgc aacactgcac caaaaccacg cacagcctct gaaatcagga aggaaggata
    38341 ggttccggga tgatacaatg ctgttggatg aaactgaata aattccatgc catttacttt
    38401 tcccatggcc cgataagcca tggcaatacc atcaccggtt gcaataacag gattggttgt
    38461 agttttataa acctgccctg ccccacctgt tgccatcacg gtaacttttg caagcatggt
    38521 ttctacctta ccggttttag tattcaaaat ataggcacca taacaggtta tgccgggcgt
    38581 atttcttttt acaatgacac ccatgtgatg ttgcgtaata atgtcaacag caaaaaggtg
    38641 tgtgtgcaat gtaatgtttg gatgttgatg aatttgctga agtagttttg tttcaatttc
    38701 aagtccggta atgtccttat gatgcaaaac gcgatgagcg gaatgtccgc cttccattgc
    38761 taaatcaaaa ctgccgcttt tgtttttgtc aaactctgtt ccccaggcaa taagttcgtt
    38821 aatgcgctcg ccactttcgg ctacaacaat gcgcactatt ttctcatcac ataacccggc
    38881 accacaactt aaagtatcct gaacgtgatg ttctaaagag tcatcttttg cttcaggtaa
    38941 aactgctgca ataccacctt gtgcatattt ggtgttcgac tcatcttcat tagctttggt
    39001 gatgatggtc accttgccat gtttggctgc ttttaacgca aaactaagcc ctgctattcc
    39061 actgcctatc accaaaaaat ctgtctttgt cattatttta actgtaaatt taaactaaag
    39121 taagtcgaat atgtttactt tcgtaaatta attgcaaaag taactattca tgtcaaccag
    39181 aaatcatcag gtatatgccg agttaacacc taatccaaac agcgttaagt ttgttgccga
    39241 taggttgttg ctttttgaag gcactgttga atacaaatca aaggccgaag caatagattg
    39301 tccgatagct tatcagttgt ttgactttac cggtattaag tcagttttta tcactacaaa
    39361 ttttattacc atcacgaagg aagccgatat tgattggtat gaaatcacca atatcctgcg
    39421 tgagtttata aaaggttttt tgaacagcgg tgaaaaatta tttctaaaaa atccggttca
    39481 ggaaattgaa gaggttcagg ttcctacttc gtcaaacatt gtgacgccac aggaaaagca
    39541 gcccctacaa aacaatacag aatctgcaat cattgaagat aaaattaaaa atatgctcga
    39601 tgaatttgta agacctgctg ttgaaggtga tggcggagca atagatttta agtcgtttaa
    39661 agatggtgtt gtcactgttg ttttaaaagg ttcttgcagt ggttgccctt cgtcaacatt
    39721 aacgctgaag gcaggtattg aaaatttgct gaaacgaatg attccggaag tatcggaagt
    39781 aatggccgaa gcagaataat tctaaaagat attaaaccat ttgtttttgt gtgccataga
    39841 aattacctgt gcttttgaat tcacatgcaa tttagtataa atattagcaa tgtgttttct
    39901 aacggttaaa gtgctgatgc ctgtaatctc tgcaatacgt ttggcatcat agccattcac
    39961 caaatgagta agcacttcac gttcacgatc tgacaacaac gtttctatta cagagccttt
    40021 agccggtttt gaattatccc tttgtgagag aagtcctaat gtcttacgtg caattgccgg
    40081 gctcataggc gcaccaccaa attcaagaac ttctgttatt gcttctttta tagaaagata
    40141 attttcatgc ttcagcaaat atcctgacgc accggattgt atagcattaa atatagtttc
    40201 atcgtcttca aaaacagtta gtacaataaa atgtatcgag ctatataatg cttttgccaa
    40261 ttgaattgtt tcaacaccat ttagtcccgg catctgaatg tccataaata ctacctgtgg
    40321 ccaattttgc atgggcaatc ctttcatctt tgctaaaaac tcatcaccgc tgttggaaac
    40381 aaacaacaac gctatatctc cgaatactgc aattttctct ccgaaatact tttgatttat
    40441 ggaattatcg tcaactaatg ctatctttat cattgttctt tggcttgata aaagtctgta
    40501 gtatatttct atttcacaat actcactttt atgtattggt aaaaattatt cttgttccat
    40561 gaggttctac aggtatccat tgtactgtcc atcccgattc tttcattcgt tgtttgacct
    40621 gcataatacc attgccataa actatatttt cgctcatgcc tttaccatta tcttcaacaa
    40681 taattgaact gctgccgtta ttattaaatt ctattttaat tgctgtacat ccactatgct
    40741 ttaagctgtt atttacgcat tctattaacg aataaaacaa attgagcatc acagaaggcg
    40801 tatatactct gtcatttaca atattttccg aaaattgata ggtgacacct gtataacttt
    40861 tcattaatcg ttgcatccat aatttaaatc ggtcactcag actaattaaa cttaattgtt
    40921 cattctttaa aacccaaatc gtattattca actgcgccac catgctttga acattttctt
    40981 ccaggttctc tgttattgtt tgcatggaat tgtcatcact tttcagtgat cttacattat
    41041 gtgtaatggc agctgcataa acaccaatat tgtcatgtaa atctgctgca atacgtttac
    41101 gttcgttttc ctgtgcctgt ttaactgcaa ctttagcctg aacacgctgt tcaaccagat
    41161 ccatttccat tttcctgttc ttgataagtt gcctgttacg atatacaagt cctaaaatca
    41221 aagaaatgag tgccaacaac aacaaagagc caattgtcac atatctattt ctggaaatcc
    41281 tcatttcctg ttgcacaatt atattctcct tcttctgcaa ttcatatttt gtttcaaagt
    41341 ttgcaatagc ttcagcagaa tttttctcgg ataaagaatc ttttaatttg atgatttttt
    41401 ccaatacttg cgcatattca ttatacattc ctgctgcttt atagttctca gcaagtgagt
    41461 tataaataaa tatcaaacgc gataaactct tgagctttgt agcaatagct attgcttctt
    41521 tagcaacttc aatacctttt tttgtttcat taatggaagc ataaaatgat gatagctgtg
    41581 ccatgtctgc aactacataa attttatcac caatttgctg tcttattttc aatgcctcct
    41641 caagatcagt agcagctttc aaaaatgact tctgcttaat ataaatatca gcacggatat
    41701 tcagggagtt ggcaacctgc gaaaggtttt gtgcaagcct tgcgtatttt aatccaagtt
    41761 ctataaaatg aaatgcagaa tcctgcatgc caatattatt ataacaagat gcaatatttg
    41821 aaaaaagata gttacacctc actaaaattt tttcatcatt agttgaatta atccctgttg
    41881 aaagccattt aatggcatca tcatatttac ccaactccat gttagcccag ccaatgccaa
    41941 tatatgatcg tgcaatccag tatgcatcat tggctttctc agcattcttc agaataataa
    42001 acccttcgtt aattgcttcc ttttgtctgc ttgtgtggat gtatatattg cttctattac
    42061 ttcgtaacca ttgtgaagcg tcataatact tttcgttttg aggaataact ttcagtaaac
    42121 tatcaaccaa gaaatccgct tcttttgtct tacccacttt cgctaaataa cgcacataag
    42181 cattttctac taaaaaatat tccggtgaac ccggaagtgc aatctgttga cctaatcgga
    42241 taaaataata gaaagtatca gcagaaagtg aatgaccacg tgcacacaat ttgattacat
    42301 tatctaattg ttcatttaac tgcgtgctgc tatgaatttt ttgctttaag ctgtcggtaa
    42361 taggtgtttg tgcatgggtg taatataatt tctgtactaa aataagcacc aaaacagaaa
    42421 aacacctgat tgcctttttc ataattaata ctgtaaaact aaagaaacag attgaaatca
    42481 aaatcttttt tttaagtcaa aaaagtcaat actcaatttg tagtatgatt catctaaaaa
    42541 aaatcccaat tactataatt taagtattta caataaaatt tattaattga aatatttgta
    42601 gcacagaaaa aggattataa caaataatta tcaaaatata tgacatgttt acaatcactc
    42661 aactttccaa attaaaaaat caccagaatt ggtgattgac ataatcaatt tctaagaata
    42721 aaattgcaaa aaataaatac gcatactaaa accaaaatta ttatgactac taaaatcgca
    42781 aaaagaaatt ggatgtttag tttaccaaga gtaaacaaac tccaattaat ttggttgttt
    42841 ctattcctat caggaatagc aatggcagaa acagagccca atgacagtaa ggctcaagca
    42901 aatgtgctaa ctctaaactc gggcatgccc ggaacggtta cactaactga atcagactgg
    42961 tttactgtaa ccatacctga agacgggaat ctgaatattc aggggatttt ctccagcaac
    43021 atttatattc agatttacga caatgatggc gttaccagtt tatttaataa ttatcatagc
    43081 ggaactttaa cccgaacggt taatgggttg gctgcaggaa cttactatat tcgattatat
    43141 aactactatt cgtcagaaac caacacctat acgataacgg caacactcac cacaactgga
    43201 ttagccaatg acattgaata caatggcaca ccggccacgg ccagcgtgtt agcattgaat
    43261 ggcagcacta ccggacatgt taattactac tacaacaatc aaaaagatac ttcagactgg
    43321 tattcagttg ccgtcaatca ggatgggaag ttaagctata ctatcactag caacggacag
    43381 aatgtttacg ccattcttta tgatggcgat gccaccacgc aacttgccgg aagctataca
    43441 accgcaacag ctacctttca acgagatggg ttggcaccgg gagtttatta tatcagaatt
    43501 tatacttatt acagtagtga atacgttgcc tatacgctat caaacatctt aacgcctcca
    43561 gtaaatgcca acgacccact cccaaatgat gtttatacac aggctacaac attgcctttc
    43621 aatggaagta ttacaggaca tataggcttt cgctataacg gagccattga tacctatgac
    43681 tggtataaag taactactaa tgctgacggt aagattcaat ataccctaac agttgataat
    43741 ggccaacatg tttatgccga attgtttgat ggtgatggta ccaccttttt agagggaagt
    43801 tatacaacca cttctgcaac gtttgaagaa gatggtcagg caccgggtac ttattacatc
    43861 agaatcagaa cattttattc caatcaattt gcatcctatc aactatcaaa caatttagtt
    43921 gttcctgtgc aggcaaatga tgcatctcca aacgatgttt atacttcagc aacagtgttg
    43981 aacttgaatg attctactac cggacatata ggctatcgtt ataacggaaa catagactcc
    44041 tatgattggt atagtgttac taccaatgct aatgggcaat tattttggac cattacttct
    44101 cataatgggc aaaatgttta tgccgaattg tttgatggtg atggtaccac taatttagcg
    44161 ggaagttata ctactactac tgctactttt aacaaaaatg gactggcacc agggacctac
    44221 tatattcgaa tcagaacatt ttattctaat gaattttcac cttatactct gagtaactca
    44281 ctatcaccgg cagtttacac caatgatatc gaaccaaacg gttcaaaacc aacagccctg
    44341 cctttagcag taaatacaac cgtgcaaggt cacatcggct tttattacaa taaccttgat
    44401 gacacgttgg attattatgc gctgacactt cctgttgatg gcaaactttc ctggaccatt
    44461 tcatctgcaa atggacaaaa tgtttacgct caattgtatg ataatgatgg taccaccaat
    44521 cttggtggca gctacacaac aacttctgca acattcacag agaataatct tgcagcggga
    44581 acatactata taaagatcag aacattctat atttctgaat ttgcttcata tgagctaagt
    44641 acctcactag atccaatgaa ctttacagta gaaaacacta cagataacga ctttgctatc
    44701 acgccaactt tgcttccggc taataccccc acaaccggtc acctcaactt ttattacaat
    44761 aatcagtatg ataataagga ctggtgggtt attggatatg acggtacggg agccatgacc
    44821 attacaggtg aattggagca aaatcatttt gatggtgatt atccatacat caattatgct
    44881 ctatataccg atactgctgc aagcgccatt gcttcaggaa cctggcataa ccctaccaat
    44941 tcctttatgg taaacacccc tgtgattggt aaatactttt taagactaac tccaggattt
    45001 agcacatttg gtgcctaccg tctgactgct ggctatactg aaaattgttc caatacaatt
    45061 accatcaact cagccgttca agcgccacct tgcttaggaa ctatcaacta taccgtatca
    45121 aacggtttgg gtaactacac cgtacagttg tataaaaacg gaattccttt tggtacacct
    45181 caaactacag catcaactgt tagcttcaat aatttaggaa taggaactta ctacctgcag
    45241 tcatcttcct ttggcgcttc aggtgcttgc agtgctaata gtgcaaacac cgtattttct
    45301 acaccaccgg ttcctaacat tacagccgga ggccctacaa cattctgtca gggtggaagt
    45361 gtagttttaa cagccgatgc tgcaaactcc tacctctgga gtaccggtgc aactacacag
    45421 gctattacag taagtgcaac aggtaactat gtggtaacag tttacaatgc agcattgtgt
    45481 agtaatactt ctgcatcaac ttctgtaaca gttaacccat tgcctccaac accaaccatt
    45541 acacctaatg gtccattaac attttgcacc aacagcaatg taacgctttc ttcaagtgtt
    45601 gctaatgact atttgtggag cactgctgaa ataactcaaa gcatagtggt aaatcttgcc
    45661 ggaacataca gtgttacaat ctctgatgcc aatggttgta ctgcaacttc tgcatctgtt
    45721 acaaccaaca ccattgcatt gcaaactttt tatcttgatg ctgatggtga cagttacggt
    45781 gatgccacta actccattca ggattgcgct gctccttcag gttatgtttc caacagcact
    45841 gattgtaatg atgcaaacaa tgcaattcac ccgggagcta ctgaactttg caatggtatt
    45901 gatgatgact gcaatggtct ggttgatgat aacattgtaa actctacata ttatgccgat
    45961 gctgatggtg atggctttgg caatcctctt gttgactctt cagcatgtgc ccctcctatt
    46021 ggttatgtat tgaataatac cgattgtaac gatgccaaca atgctatata ccccggtgct
    46081 actgaagttt gtggcaacgg cattgatgat gactgcgatg gtcagattga tgaaggttgt
    46141 ggcagcaaca cctggtatgc cgatgctgat gctgacacat atggtgatat taataacact
    46201 gtggtggaca acaatcctac tcccccatca ggttacgtgg ccgacagtac tgactgtaat
    46261 gatgccaatg ctgcaataca ccccggtgct actgaaatag tgtgtaacgg tattgatgaa
    46321 aactgtaatg gtatggctga tgacttagga acaccaagca cagatccaac atccatctca
    46381 agcgatgttg tattcaatga aatttgtatt ggtggcaata ctaccttaac cgttaatggc
    46441 ggaagccttg gtaccggtgc ctcatggcaa tggtacagtg catcctgtgg tggtactcct
    46501 gtgggttccg gaaacagcat cactgtctct cctgcaacgg caactaccta ttttgttcgt
    46561 gccgaaagtc catgtggtaa ttctgcctgt gttagtctta ctatcactat caaaacttcg
    46621 ctgcccgcac atgctgtcgt tgttcctcct ataagcggtt tacctgctaa tgcctgtccc
    46681 ggttctactg ccaacatcag cattcctgcc gttgccaatg cttctcaata catttgggat
    46741 ggcccttcag gtacttactt caacggtaat ccattgaaca cttcaccatt tacatcctct
    46801 acaccgagtg tgcaagttac tttcggaaca accaattcat cattctataa cattggtgtg
    46861 caggctgcca atgcctgtgg tgcaacctta cgcaaaattc aaaaagtgcg ttacaaaatc
    46921 agtacccctg ctgctatctc tggagcagtc aatgcctgtg ctaacagcag caactcatac
    46981 gctattgccg cagttacagg tgctacacag tatttgtggt caattacagg agatgcaact
    47041 gtgattggaa acggcactac cgtaaccgtt aacttcgggc ctgcctggac cggtggtaac
    47101 ctttgcgttt ctgcacaaac aacttgcttt acttctcctg caaaatgtat gctcatcagt
    47161 gcttcgtcaa ctgctttaaa tgccatcaac ggaacattca ctgcttgccc aggaagtgtc
    47221 atcagctata acgttgctcc ttctgcaggt gcttcatact atacctggac attgcctgca
    47281 ggtgcaacag gaagttctac taccaacagt atcaatgtta cctacggacc atcatttaat
    47341 agtgccggca acatttgtgt aactgttaca agtgcctgtg gtgttacttc tgctcctaaa
    47401 tgcaaaactg tggctccggg acttcctgca agaccacttg ctattactgg accctccaat
    47461 ggtctgtgtg gtcagtcggc atcgtatagt gtagtttcca ctccgggaat tacttataac
    47521 tggactgctc ccggaagtat ttccggtaac ggcacaaatg ccatcaccgt tgcctatggc
    47581 tcgttcacta caggttctgt ttgtgtaact gccagcaaca gttgcggcac cagtgcagca
    47641 cgctgcatca ccattaaagg tgcacctaat actccggtgg ccttaactgc taacccagct
    47701 acctggtgtg ccaatacaca aggtattgaa ttcactgcta atgtgagcaa tactaccggt
    47761 tcttatacac tggcatggac atatccttca tcacctgtag ctacctatat ttccggtggt
    47821 ggcaattcca ataccttgac attagattgg ggtacaggca acggtactgt gattgttaaa
    47881 gccagcaata gttgtggtga aggttctaaa gcatatttag tgaatatcgg ttgtaaagaa
    47941 gcacagcaag ttgatgccgg tacattatct gcttatccga accctactgc aggcatgttg
    48001 aatattgaat tcaatgcagt tgaaaagagc aatgcttcag tatcggtaat tgatttaagt
    48061 ggcagaacaa tcatgaattc atctcagact gtaactgcag gcagcaataa tttgcagctc
    48121 gatttaacga atgtagctaa aggtgcttac atgattcgtg ttaaagcaaa tcagtttaca
    48181 aaacaaataa aagttattgt agagtaattt tttagtatat tatcttataa aagaggctgc
    48241 caaatcaggc agtctctttt cttattacta ttgtaaggta ttttaatcag tattcttatg
    48301 agagcttcta tacttttaac tgttaattta gtattcacaa catttgctgg ttttgcgcag
    48361 tggagtacaa caactgttca atcggcacgc agtttattag cagccacttc tatacaagat
    48421 aaagctatgt ttggtggtgg cattggtatg ataagtgttc ctccacctgc attagatatt
    48481 tatacacaat caacaaatca atggatgcag tctcaaattt cacaaggtcg cacttcatta
    48541 gcagcagcgt caacaggcaa ctgggcaatt tttggtggtg gcgccaacga ctggattctt
    48601 tctacctata atagagtaga tatttataat ggcactgtag gtagttggac tgatggtgat
    48661 tttctttctg ttaatcgtgc caaattagct gctgcaggtg caggaagcaa aatacttttt
    48721 gccggtggtg aatttgatat gattaatgca gtatatgata cagttgatat ttttgatacg
    48781 cttactcaaa gttggtcaac aagtgcttta agcgttgcac gtttcaatct tgccggtgct
    48841 tctgcgggca ataagatttt gtttgccggt ggagaagacc tcacggggac agtttatgat
    48901 aatgttgata tctatgatgt aacttcaggt atatggacca cagcaaatct gtctgttcca
    48961 cgtacaagat tagctgcaac agcaatgggc aataaagttt attttgcagg cggcattaac
    49021 aatatcggag ttgtgagtaa tgtagttgat atttatgatg tcatatccaa cacatggact
    49081 acagacacac tatcacaggc aagacaatac ttaactgctg ctgccaccat ggggaaagct
    49141 ttctttgcag gtggaacaga tgcaggttta ctaacaggtt caaatattgt tgatattttt
    49201 gatactgcca ctgcaacttg gagtattgat tctctgacga caggacgtta tttatgtgca
    49261 gcaactactt taggaaatca attcctgatt agtggtggta ttccttccgg cacaggattt
    49321 ccactaaata cagtagaaat ttataccatc gctactagtg gaatttcacc gatagaaact
    49381 tcaaaaacat taagtgttta tccaaaccct gcatcacagc aaattatgat taaatacgat
    49441 gattttaaat ttggtgatgt tttaaatata actgatgtaa tgggaaaaca tttgcttagt
    49501 ttgcaaatta caaacaatga aacctttgca gatattagta atttgagtga tggaatttat
    49561 tttgtgcgac ttaattcagg tcttagaaac attgcatcaa aatcacaaaa aattattatt
    49621 catcactaaa tcaagtattg aattttaatt tttgcctgac agtttacttt caataataat
    49681 ttttctgatt aaatgctgtt tttcgcattg tagttcgtta atgctctcag cattttattg
    49741 cgtttaattt tttataggtt taaaatattc ggcttcgaac cgcaataatc agcttgaaat
    49801 attttaaaat gtactcattt aaattcacca taattggtga ttgaccggct tttataccct
    49861 ccataatttt ggtggctgat aatctactaa aatcaaattt caattattat ggaaaacaga
    49921 aacaacaaaa attatgtgca gtacaacctt gcacgcatgg ctttcattct atttgcatta
    49981 atgacaggcc tgcagttaca agcgcaaact cctccgttta cacgatcaac ttttcagtcc
    50041 gcctatgttc caataagcat tggtggtgga gcaacttctt caacggctac cggagacaat
    50101 actatgcaaa ccggaatacc tattggcttt aactttgggt atggtgacag tactttttca
    50161 agcattagtt taaacaccaa tggattgact tggtttgata cacctgctcc caatcctgca
    50221 gcgggtcatt tttttattgc aactaatacc gctcccaatc aaactctttg ggcatgggcc
    50281 aatgatgatt taattgacga tgcatcatcc gacattttat atcaaatact ggtgctgcta
    50341 ttggaattga atggggctta ggaggtaacg gcaattttat tgatgccata accggttcat
    50401 caacgctaag ccaccacatg ctaagtcctt ttagcggatg gcctgcttac aacttcagat
    50461 ttacaccggg cgctccttca acaattcctg caggaaccta taacgtaggt gttggccaaa
    50521 cttataacag tttaacccaa gctgttgctg atattaatca tcgtggaatt aacggttcag
    50581 taactctgaa tcttaccgat gcactatatg ataccactgc ggcaaacggc agcaatatat
    50641 tcccaatttt tgtggcaaca cccaatgccg atagcatcaa cacgcttact ataacaaaaa
    50701 ttggtacccc cgctaccttg gcttatcgtg gttcaagcat aaccagtaca ccggttatgg
    50761 cctttggaac tgctattggt cttgctgctt taaatcatga tatggagcca attcttgggg
    50821 tttgtggatc atatactact gtcagtaata ttaatttaat cagccatggc tcaccacaag
    50881 tagtagatta tggtctgtta cagtttgata tggctgaagg caaaggcgca cagcataatt
    50941 tatatgataa aataactgtt gacctagatc gcaactatgg tgatgcacat ggtatgtatt
    51001 cagtagctac tggcggtggc tggaataaca caaacaattc atataatacc tggcgcgata
    51061 ttactattaa agattgtaat tatggttttc gaatatctgg agcatattat agtactgaag
    51121 ttggtaatca gcttatcact tcttcgtgca acacctataa ctatattggt gaccccaata
    51181 cacctgatga tattattagt agtttgaata actatggggt atggattacc aaggtagcag
    51241 gttttaccat gagaaattgt atcgtacaga atataaccac cacttctacc attggcatac
    51301 aagcagttcg aattgacggt gcaataaatg gcatatctga gattagcaat aatatcattc
    51361 gtactatcaa gcgcatcaat ccacaaatat ttccaactaa ttccattgat ggtatgtata
    51421 tcgactgggg gaacaataca ggattattta aagtatttaa taacagcatc agcaatcttt
    51481 taaccacata cgcgggtgcc cctactgatg tggttgccac aagagggttt gtatttggtg
    51541 atggtggtgc tggtccgtat ggtgttgatt tatttaacaa cagtgtaagt attgacggca
    51601 gtacattccc caatgcctca aatttatgcc ttgagacttt ttcaaatgat gcaacattca
    51661 ctattaaaaa caatgtattt gcaaatttca ccacaggaca aacaggggtg gcaacacata
    51721 cctgcttctt cacacaatat acagaccact atggaaacgc tgcttcaatt tcagactaca
    51781 ataattatta tgtagctgac acagccaatg gcgcaccttt taaggcaact acaacaatgt
    51841 atagtactca ggcagcat
//
LOCUS       DAOQNP010000008        51314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_188_length_51314_cov_8.180007, whole genome shotgun sequence.
ACCESSION   DAOQNP010000008 DAOQNP010000000
VERSION     DAOQNP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 51314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_51314_cov_8.180007"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(378..1307)
                     /locus_tag="PKY09_02115"
     CDS             complement(378..1307)
                     /locus_tag="PKY09_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB11677.1"
                     /translation="MLFTIEKTNQAEKADMLAGNKVMRWVYLLVLASMWGSSFILMKR
                     GLNSFSANQVAAIRMFISCMVMLPFIAPYIKDIPKKLWLFVMATGLLGNGIPAYLFTH
                     AETGISGSMAGMLNSLTSMFTLVVGSLFFATQFSKRHLLGVIIGFAGAASLIWLNAED
                     AQSAEPWKGVYVLIATVCYAFSVNILRNKLASINAVTLTGAALLFAGIPSGIYLFSTD
                     FISRFQTEPLAWNSFFYIALLAIMSTVISTVLFNHLIRISSALYASSVTYLIPFVAVL
                     WGLYDNETFNMLYLLALFVILTGIYLTNYQKKV"
     gene            complement(1246..2535)
                     /locus_tag="PKY09_02120"
     CDS             complement(1246..2535)
                     /locus_tag="PKY09_02120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB11678.1"
                     /translation="MSTTTGFDSLKLNRQLLNAIGDLGYETPTEIQQKVIPPALSGQN
                     IIGIAQTGTGKTAAFILPLLRTLNYAQGDEPRALILAPTRELAIQIGNVAIQMGKYVD
                     LRVVTVYGGKGFSDQKKKLEAGCDIVVATPAQAMELYLSGHLVLKKIKHFVMDEAERL
                     MDFGFTGQIYSLLEVLPRKRQNMLFSATFSERVKKISDDFMEFPVFIDVVPQVKTAAK
                     IEQFIYTVNGFQTKLNLLNFLLTDKENFNKVIVFCKSKKNANSVAEMLLMKFEADGIN
                     VLHGDKTQQTRVNAINRFRDEQIRILIATDVAARGIDVPGVSHVINFDVPLVYEDYIH
                     RIGRTGRAFASGFSVTFVEPQDEWHIKKIQKLAGISIKRLNLPDSVEQIQPGKEETKL
                     REMEIDRQKRKDNPEFKGAFHDRKNKSGRKGRHAGRK"
     gene            complement(2532..3284)
                     /locus_tag="PKY09_02125"
     CDS             complement(2532..3284)
                     /locus_tag="PKY09_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNB11679.1"
                     /translation="MQEFIAPLLTADGIVSLITLLFMEIVLGIDNIIFIAIICGYIPD
                     RKEQSRARIIGLLLALVFRIGLLFTISWIARMINPLFYIGSFGVSGRDIILFAGGVFL
                     IIKTLKEIYHKFKDAEAHKSGNPSRKMTMIQAIFQIMVIDVVFSFDSIITAVGLSNQI
                     VIMVGAVIGAMMVMIAFAPYVSDFINKYPTLKMLALAFLVVIGVILVVEALHIHFDKS
                     YVYVALGFSLLVEMLNIKMRSLSHRKKADEPK"
     gene            3436..5331
                     /gene="asnB"
                     /locus_tag="PKY09_02130"
     CDS             3436..5331
                     /gene="asnB"
                     /locus_tag="PKY09_02130"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB11680.1"
                     /translation="MCGIAGIASFTKSAKDFQQHLRSASSCLRQRGPDNEGFFIDDNV
                     ALAHRRLSIIDTSAAASQPFSDASGRYTIIYNGEFFNYRHHRSQLESQGVSFVSESDT
                     EVLLQLYIRKGINCLQCINGFFAFCIYDNLEKTVFLARDRMGVKPLIYYSDNDKFIFA
                     SELKALMQFPIERNIDQQSLYHYFQFNYIPDPWTIYSNVNKLSPGNYLLIDLKKNSVI
                     EEACYYSIPETEMLDMPDYQSAQLRLKELMTDAVGLRLVSDVPLGCFLSGGIDSSVVT
                     ALAAQQVNKLNTFSIGFKDEPFFDETSFALRVAEMHNTAHHVFKLTNGELFENLADIL
                     NYIDEPFADSSAIAVFILSKYTKKHVTVALSGDGADEIFGGYQKHRAEWLIRNRPGLT
                     PLLKMAASVLPSKFSSRENSIKNKIRQLHRFAEGATLSNKERYLRWCSFTTANYRNEL
                     LLKPFANDLKNRNQQLSTYVKHQTSMNDVLLSDMKLVLTGDMLRKVDSMSMANSLEVR
                     SPFLDYRVVDFAFSLTDKYKIDNKAQKKIVKDAFRLLLPEEIYHRGKRGFEVPLIKWF
                     QTELRGKIDFLLGEEIIQSQGLFNLNSIKQLRHDVMNNPSGESQAKMWALIVFQNWWL
                     THHLKSN"
     gene            5334..6524
                     /locus_tag="PKY09_02135"
     CDS             5334..6524
                     /locus_tag="PKY09_02135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11681.1"
                     /translation="MPRVLRIINRLNLGGITYNVTYLTRFMQPEFETMLVSGVKTEDE
                     ESSEFILDQYGIKPHYIREMKREINFSSDRKAYAELKSLIKKFKPDIVHTHAAKAGTL
                     GRLAAFSCNVPVVVHTFHGHVFHSYFSPLKTKLFIEIERYLAKKSSAIVTISELQKHE
                     ICNVFKVSAPEKTHIIPLGFDLNRFRENKDEKRIAFRKMYKIADDVVAVGIIGRFAAI
                     KNHAFFLEAMQSVINNTSQKVIAVLIGDGEEHESILDCCRKLNMPFAKDASNTETKIV
                     LTSWIKDIDRALAGLDIVALTSHNEGTPVSLIEAQAAGKAVVSTRVGGVENVVAEGST
                     GFLCNKGDLQSLVNQLLQLVEDMGLRNKMADNGWEHVRNKFHYTRLVNDMKDLYTELL
                     SAKQ"
     gene            6664..7374
                     /locus_tag="PKY09_02140"
     CDS             6664..7374
                     /locus_tag="PKY09_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNB11682.1"
                     /translation="MFKTKKDFVSQPDRTIGNVEYKIAPNDILSCSVYTNDGIKLIDL
                     TANSNSLNESANLSTAMNGSNQYTVDIDGFVKLPIIGKVKIQNLTTREAEKLLEDQYA
                     TYYNKPFVIIKVLNRRVLVFPGEGGAGKVVTLTNENTTLIEGLALAGGISQNGIAKKI
                     KLIRGDTRNPQVTIIDLSTIEGMKESNLLLQANDIIYVEPRKRISQGILSELAPIVAI
                     FSGIASIFIAYDVIKRNQ"
     gene            7381..9813
                     /locus_tag="PKY09_02145"
     CDS             7381..9813
                     /locus_tag="PKY09_02145"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNB11683.1"
                     /translation="MPKKQITPLNEEFDFKLFVTIAQRNVEWFLMFMTLSVIIWYVIV
                     RYTAPMFESSALMKLNTDNNVTKALNITRDVYQEQNNELAGNLELIRSSIIVSKAIAT
                     LPLRVTYISKGTLLENEMYKSSPFEVNFLIKDSLWLDRPFYLQFINEKSFVLSYIPVG
                     EKDQIEEENENGKWIHLKGISLQINVINYQSILKHQSQLKKNTFKFIINSDKALIKDF
                     SSKLSVIMANPDAKTINIKVKEKNATKASDMSNAISQEFLKFDVLRSREATDRILDFI
                     DTTLYRVNNDLSESEINLERFKSHNRIITPELLATDLSSKMSQIELRLTDYTLNLLAI
                     SELKKAIESEKKVDEKMLSLAGIYKTDEIKEALNSLNVLLDKREEALIQVTDKAEYVK
                     MLNGRIQRLKEYFLQSVTYEEEGIKTKKAELESQYRKLNSNYLGIPEQQTEFERLQRI
                     FNVNEKFYSLLLEKKAEFSITRAGFVSKNIILEAARPASAPYYPNKSLILSSCIMVGF
                     VVSFLLIFIKYLFYNQIGSLEEIAQYTDAPLLGIVPRYKREIPISQLLVDKSPKSVIA
                     EAFRSIRTNLQFINNDEKPQLMALTSTISGEGKTFNAINLAGVISFSGKKVIILDLDM
                     RKPKIHLGFNVENNRGMSTILIGKDTIENCINHSALENLDFITAGPIPPNPSELIISP
                     KMDELLEYLKLRYDVIIADTPPVGVVSDGIPMIQKADFPIYILRANYSRKMFIHNINK
                     IISENKVPRLSVVLNSVDQTKLKYGYGYGYGYGYGYGYGYSYGYGYGYYSEDDKKPTG
                     IWNRIISFFIRK"
     gene            9820..10362
                     /gene="rfbC"
                     /locus_tag="PKY09_02150"
     CDS             9820..10362
                     /gene="rfbC"
                     /locus_tag="PKY09_02150"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNB11684.1"
                     /translation="MKIIKTALNGICIIQADVFSDERGYFLESYQKEKLKNLGIEAEF
                     YQDNFSLSAKNVLRGLHFQNPPFDQGKLIKVICGAVLDVVVDIRKSSPTYQKNFTIEL
                     NAEDHLMMWIPPGFAHGFVSLKENTLFYYKCSKPYHKDSEAGIIYNDPVLNINWSVNQ
                     PVLSEKDKILPNFSNATILF"
     gene            10462..11598
                     /locus_tag="PKY09_02155"
     CDS             10462..11598
                     /locus_tag="PKY09_02155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNB11685.1"
                     /translation="MKDIVNHNYHLFIYAVLFLGSLAFSFLINSLFLRFFFTLGIRNT
                     NDGTVIRWGSMSKPAVGGLSFYIVFLLSLATYSIFFNNNETLYNSQFMGFIMAMAIGF
                     LIGLADDAYNTKPVLKFLAQVSCAFSFIFTGTYIDLFDNMILNYLLTIFWVVGIMNSI
                     NMLDNMDGITATVSLGIIISAMVVITMSNDINNLYLASIIGLSAGLLGFLYFNWHPSK
                     MYMGDTGSQFLGVFLAGIGIMFFWNNPYHITESLPIKKILTVAIAFLLPLVDTTVVVV
                     NRVSKGKSPFIGGKDHTTHSLAYLGLKDNMVSWVYAGISLISVILVYVLNFLITTWDI
                     LYTVIFAGYFLIVLAVFFYTTSRIRAASKKPVEATPAYSASHYN"
     gene            11666..12274
                     /locus_tag="PKY09_02160"
     CDS             11666..12274
                     /locus_tag="PKY09_02160"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB11686.1"
                     /translation="MTIYIIIANVLLSLVCFRNNELLSKMIFNPYMVSQHKQWYRFIT
                     SGFVHADFLHLFLNMFVLYSFGPIVENHFVLINGESGSVLFLIYYLMGLVVSILPTYF
                     RNKDNQFYNSLGASGSVSAVLFSFILFFPLQKIYLYGLLGIPGILAGVGYLWLSSYMD
                     KKGGDNINHNAHFWGAVYGFVFPITFAPGLVLSFFDQLTGMF"
     gene            12274..12786
                     /locus_tag="PKY09_02165"
     CDS             12274..12786
                     /locus_tag="PKY09_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNB11687.1"
                     /translation="MINSNKAIFFDRDGVINDNSLYYTYKIADVVFNDGIFEAMKLFQ
                     DNGYLIFVITNQSGIAKGVYSHNDVCEVHSFMSSEFKNRNITITNFYYCPHYPVQSKC
                     ICRKPDSLLLEKAIARYKIDKDKTHFIGDMETDMIAANRAGVIGHLIESNSNIVELAK
                     KICQHDVGVL"
     gene            12770..13597
                     /locus_tag="PKY09_02170"
     CDS             12770..13597
                     /locus_tag="PKY09_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNB11688.1"
                     /translation="MWAYYDGQWVDSTTPVLKLNNRGFIYGDGLFESIRCSRGKAKFL
                     SLHVERLLAGAAVLSLNCDFITEKFLNEIIFEINQRNKFLAGSKLRLVLTRNSGGTYL
                     PEQNNTSLCLLPSAEVESEYIAPENFVELGVYREQQKPINKLSGFKNCNSLLYVLASI
                     YSQEMKLDDSLILNSKNMLCEATASNVFWLKGNQVYTTPLSSGCVNGVMRKVIIQITN
                     AIEQDISIEQLADADEIFLTNTGSGIRPVKKMQGKVYETSYGVQLTNRLNDFIILSR"
     gene            13597..15000
                     /gene="mnmE"
                     /locus_tag="PKY09_02175"
     CDS             13597..15000
                     /gene="mnmE"
                     /locus_tag="PKY09_02175"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNB11689.1"
                     /translation="MLQADTICALITPPGRGAVGVIRISGAKTFEIISRIFIPANNSL
                     SIPQLEHQRIYFGKIVDNNDLIDEVLVSIFKAPATYTGEDVAEVSCHGSGYIRQRILQ
                     LIMQSGAVMAKPGEFTMRAYLNGKMDLAQAEAVADIIEAENSISHHAAMQQMRGGYSN
                     EIKSLRDQLIKFAALIELELDFSEEDVEFASRAELKTLVLNVQHKIEHLLKSFELGNV
                     LKNGVPVVIAGKPNVGKSTLLNALLNEERAIVSDIAGTTRDTIEEELNVDGIRFRFID
                     TAGIRKTDDVIESIGVKRTFAKASEAKIVLYLFDPMESSPADLQNEILHLQHELNLSD
                     IDLKKTILLVANKSDALNVNFDVHSYDKCGNVILISAKNKLNLNILKEILIERSGIDG
                     VALNESVVTNARHAASLSQAKAALDLFLHGMKEGISNDLLSTHIRDAIYSLGEITGQV
                     STEDLLDYIFSQFCIGK"
     gene            15051..15569
                     /locus_tag="PKY09_02180"
     CDS             15051..15569
                     /locus_tag="PKY09_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNB11690.1"
                     /translation="MAVQKLLKDSGLIVSDIQLGEATIETRPTEAEKKQLQDNLNYLG
                     FELIDDKKSRIIEKIKTVIIDLVHHRDGELKMNLSDVLSHELNHDYSFLSNIFSEVEG
                     TTIEKYFIAQKIERVKELLVYDEMSLSEISYKLNYSSVGYLSNQFKKVTGLTPSHFKQ
                     IKSNKRKSLDRI"
     gene            15650..18070
                     /locus_tag="PKY09_02185"
     CDS             15650..18070
                     /locus_tag="PKY09_02185"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNB11691.1"
                     /translation="MEKNTEDTIYLPLENVESEHCALIVDKGLSKVAGVQSHKVELNN
                     RRAAITVKNKESLGDAIATIKGLGYGVTTVRRKFPVLNMSCASCAVSVESMIQSVNGV
                     ISVSVNFATAELTVEYLPNMTNPVALQKVLQSVGYDLLIGDEASQQNSIENIQQEKFK
                     KLKQKTVLAIVLSIPVVVIGMFFMDLSYANEIMWLFSTPVVLWFGKDFFINAWKQAKH
                     RSANMDTLVALSTGIAYLFSVFNMLFPQFWHQRGLHAHVYFEAAAVIVAFILLGKMLE
                     EKAKGKTSSAIKKLMGLQPKTVIVIQSDGTEKQTFVEDVTVDDIILVKPGEKIAVDGT
                     LIKGSSYVDESMLTGEPVPVLKNINDKVYAGTINQKGSFQFKALKIGKETMLAHIIKT
                     VQDAQGSKAPVQKLVDKIAGIFVPIVIGIAILTFVLWQIFGVDNGMVQGLLAAITVLV
                     IACPCALGLATPTAIMVGVGKAAENGILIKDAESLELAKKVTAVVLDKTGTITEGKAV
                     VTDVYWHNDNSGTASILYSIEKKSEHPLADAVVKNFSKTEVVELSSFESVTGKGVRAT
                     YLGENYFVGNMTFIKEQNISIDDMLLEKATAWTKEAKTVIWFTDSEQVLSIIAISDTI
                     KTTSLSAIQQLHQMGIEVYMLTGDNESTAKAIAEKTGIDKFQANMLPQDKAAFIKELQ
                     QQGKIVAMAGDGINDSTALATADVSIAMGKGSDIAMDVAKMTIVSSDLNKIPMAIRMS
                     KQTVATIKQNLFWAFIYNVIGIPIAAGILYPVNGFLLNPMIAGAAMAMSSVSVVSNSL
                     RLKLNKYK"
     gene            18156..18392
                     /locus_tag="PKY09_02190"
     CDS             18156..18392
                     /locus_tag="PKY09_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11692.1"
                     /translation="MENKKQFQFKTNINCSGCVAAVKPHLDKLINSENWQVETTNKDK
                     ILSVITNEVLEEEIIKTVQKAGFKIEILKSNDVG"
     gene            complement(18510..19031)
                     /locus_tag="PKY09_02195"
     CDS             complement(18510..19031)
                     /locus_tag="PKY09_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HNB11693.1"
                     /translation="MRKSILALASAAFIFAACNQSKQPETPATETTPPAATEEAAPAV
                     APPTEPAIVELSGGDDMKYDKTEITVKEGQTVKLTLKHTGKSPKEAMGHNFVLLASGT
                     SLQDFATAAIKAKDNDYIPADMASSVIAHTKTIGGGETTEIEFTAPSKGTYDFLCSFP
                     GHYGTMKGHFIVE"
     gene            complement(19336..20517)
                     /locus_tag="PKY09_02200"
     CDS             complement(19336..20517)
                     /locus_tag="PKY09_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNB11694.1"
                     /translation="MNKLVNITACVIIPTYNNAQTLKKVINGVLIFSSGNDVIVINDG
                     STDDTTEILKQYDEQIILLTNKTNKGKGYSLRKGFEEAIRRGFKNAITIDSDGQHYPE
                     DIDVIIKQALKYPEALLMGSRNMDQESVPGKSSFGNKFSNFWFWVETGQKLPDTQTGF
                     RLYPLEPLKKIKLFTTKFETEIEVIVKLAWKDVKIIPVNIRVLYDMKERVSHFRPFRD
                     FTRISILNTYLVTLTLFYHLPLRLFKYIKKKGIFTIIKDEVFRSDESIFKKSLSVGFG
                     VFMGIVPIWGFQLLVGIPLAVLFRLNKVLFITAANISIPPMIPLIIYFSYYLGGFFVS
                     NDLKFQSMTDITLQSIHANFVQYATGACVLALITGIFFMALTYITFLVLKFKNRITDK
                     T"
     gene            complement(20514..20867)
                     /locus_tag="PKY09_02205"
     CDS             complement(20514..20867)
                     /locus_tag="PKY09_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase"
                     /protein_id="HNB11695.1"
                     /translation="MLKDFYTIINKISGQNSEYRIKLNPKHEIFKGHFPGQPVTPGVV
                     LIQMTTELTQNSIEQNVKLKQVINSKFLNVMNPEKNPEVTFKISISKNDQLYKVVTSA
                     EDMGVCFFKMTAFYQ"
     gene            complement(20869..21492)
                     /locus_tag="PKY09_02210"
     CDS             complement(20869..21492)
                     /locus_tag="PKY09_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNB11696.1"
                     /translation="MRFSITFLLFIWIPVLVKAQLEPLNNPAELTEKLKQASISNNSI
                     KADFTEQRFMSALKEPQTSYGIFYYKKENKLRWEKTKPSSYVFISENNKVRVKENNHE
                     KDVSTYNQVVGRIKDLMLTLVNGDFSNGKQFDPQYFQTKTEYIVKLKPRNKRMSNAFD
                     YIQLSFNKKNLLLEELAFYEKSGDRNIMKFSNQMVNQELNDSLFKNF"
     gene            complement(21489..22241)
                     /locus_tag="PKY09_02215"
     CDS             complement(21489..22241)
                     /locus_tag="PKY09_02215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB11697.1"
                     /translation="MKHKIITALFILCIIALFVFLYQDKSFFYLLATLIILYLTITAL
                     GSYQIKWNYFIKSVNTGKPEGISLTFDDGPEPKVTKKILDVLELAGVKATFFLIGKKA
                     EQYPDIINLIVSKGHTIGNHSWSHHAGIGMFSKQKLLADFNQCSQKIEEITGQKTVYL
                     RPPFGVTNPRYENVLKNTGMKSIGWSIRSFDTSAKNEQKLLHRITSQLKPGAIVLLHD
                     TKDITLDILPALLNYCKQNNIAIKPLAELIET"
     gene            complement(22231..23196)
                     /locus_tag="PKY09_02220"
     CDS             complement(22231..23196)
                     /locus_tag="PKY09_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNB11698.1"
                     /translation="MYCIQSAATISHQPSFASIGFSSRLSKDIGSLIKPDYKDFVPPS
                     LIRRMSEILRMSVACSNLVLKDAGIKQPDAIITGTGLGCLYDTEKFLNNAITIEGGLL
                     PPTSFIQSTHNTIAGQISLVLGNHQYNMTHTQNSLSFEHALLDAMLMLDENDGNILIG
                     SADEVIDPLLPIAAYFKLNHFPLTSGTSFYIISKNKNEKTLAVIEACHTTAFYSSLEN
                     EIKDFLSKTGEQQIDFILNDGCNKDVSRLIEALLPGKCIINYSEICGLYATNSAFALH
                     LATDIICNKTKQVYDRTINSHQRALIINSLLPERAGFIMIKSNEA"
     gene            complement(23199..24377)
                     /locus_tag="PKY09_02225"
     CDS             complement(23199..24377)
                     /locus_tag="PKY09_02225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNB11699.1"
                     /translation="MKVFVTGIGVVSAIGLTVEENFRSLLNKKSGITECKFPDDQKSI
                     YTGRIAKSNSELKQLLSITDGYYSRTSLLGIIAAEQAMGNNFHNKKLLTAFISSTSVG
                     GMDETEKYYRQVLKNLPVDFNLTRTHDSGNTSENIAAYLKFSGYINTLSTACSSAANA
                     IMLAARMIAQGKMDRVLVGGTDPITDFTIKGFQSLMIYDNEWCKPFDHNRNGLNLGEG
                     AGFLLLEGDRSIKISGNEPICSLTGWHNAADAYHQTASSPDGKGATIAMKNALNKAGL
                     ESKEISYINAHGTGTKNNDLSESIAIKNVFGNAIPPFSSTKAFTGHTLAAAGAIEAVY
                     SVLAIKNQVHFSGLNYNDPLEETLMRPVTEPTQAVTKHVMSNSFGFGGNNSSLIFSRI
                     "
     gene            complement(24384..24641)
                     /locus_tag="PKY09_02230"
     CDS             complement(24384..24641)
                     /locus_tag="PKY09_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNB11700.1"
                     /translation="MDNLKEELKKHLITQLNLEDKTPADIMDEAPLFGDSGLGLDSID
                     ALELIVLLDKHYHIKLTNPEEGKSVFYSINTMAEYIEKNKK"
     gene            complement(24643..25254)
                     /locus_tag="PKY09_02235"
     CDS             complement(24643..25254)
                     /locus_tag="PKY09_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11701.1"
                     /translation="MKISGCCRIHQNKVLVNGKKYFASDNSQHDELSMQLYRFLKTEY
                     PKFYKMDLLSKMGFLATEIICQTTSAISKFADDEIALLFANSHSCSESDERFNHSFKV
                     EKSPSPAQFVYTLPNILLGELAIRNKWYGENLFTLLPAFSAEYFSSQAGIFVPQKAKA
                     LLCGWVNLFPVTDVFVFFAESNSETAQEILDSKEIEHWYNNIK"
     gene            complement(25251..26327)
                     /locus_tag="PKY09_02240"
     CDS             complement(25251..26327)
                     /locus_tag="PKY09_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNB11702.1"
                     /translation="MIYIGAETILSPNGNTKDCFRKIAEKKSGVSKITGNGFNGESFY
                     CSMFSDSRPDFNTLAVQTIEQLLEKSKANLLTEESTLLIISTTKGDLSKGFENAIVSP
                     AKFLYEKYKLKHFPLSISNACASGVMAINAAAAFIENKQYQNVIVLGIDMITDFIVYG
                     FQSLYAISDEPCKPFDSERKGINLGEGAGAVLLSANKDVFTTTPVCYLSGSSSNDANH
                     ISGPSRTGEGLFRSVNKTLSASAIKTENIDFISAHGTATVFNDEMEAIAFNRLNMQHI
                     PVNSFKGFTGHTLGAAGVIETALCITCMHENTLLPSAGYYTKGTSIELNLITQAASAT
                     INTVLKTASGFGGCNASLLLQKIK"
     gene            complement(26331..26759)
                     /locus_tag="PKY09_02245"
     CDS             complement(26331..26759)
                     /locus_tag="PKY09_02245"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB11703.1"
                     /translation="MKQLLQVEIPIKVRFSDTDAMGVVWHGNYLRFFEDAREAFGIKY
                     GLTYLDVYHRGYFTPIVKSSVEHKSPINYGEEVVAIAKFHPTPSAKIMFDFDILNKTT
                     GKLSARGHTTQVFLSVVEREMELVKPPFYIEWEKSIGIKH"
     gene            complement(26756..27172)
                     /locus_tag="PKY09_02250"
     CDS             complement(26756..27172)
                     /locus_tag="PKY09_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11704.1"
                     /translation="MIIEKENIESYIPQRFPFIMIDSLIHADESGFISSFQVMEENIF
                     SENGFLTEPALIENIAQTCAAGFGFIGQKNNEGPSIGYIGSISNLTVHQLPDYGTTII
                     TKIEITNSLGNIFLIKGRNTCNENLLIECEMKIALA"
     gene            complement(27169..28038)
                     /locus_tag="PKY09_02255"
     CDS             complement(27169..28038)
                     /locus_tag="PKY09_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB11705.1"
                     /translation="MAQWDGRSRGTVLGYRIFVFLIQYGGVKPAYFLLRFVTYYYFIF
                     AAKPKKILINFYRKALKVNEAAAKNYCRKNFYFLGQTLVDRTAFLLGKGNVFSHHFEN
                     EKYLIEMQEQKKGGILISAHVGNWETAGNLLKSRVSSTIHVLMVDAEAKKLKQYLDNS
                     TGGSQFSIIAVKDDLSHIVKVNNALAANELIAMHGDRYLPGVKTIELDFMGGKAKFPY
                     GPFLLASRFSAPVTFVFALKANDFHYELSATEPITEKKSPEAIAALYVEELEKKVKKY
                     PEQWFNYYDFYSQ"
     gene            complement(28043..28294)
                     /locus_tag="PKY09_02260"
     CDS             complement(28043..28294)
                     /locus_tag="PKY09_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNB11706.1"
                     /translation="MNREEIITTTKRFLSEEFEVDETLIENQKNLKDTLELDSLDYVD
                     LVVVIEENFGFKTSGEDFKSISTFEDFYNFVERKLAAMA"
     gene            28702..29610
                     /locus_tag="PKY09_02265"
     CDS             28702..29610
                     /locus_tag="PKY09_02265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB11707.1"
                     /translation="MLANIAKRILPDGTRQVVSNFYYEQKLFKAARAIAMVKGPSEVL
                     DEKHFEPLFDQFPVGDPVLYDAVSIKERGIQRAEDLIGFMHNYSGASYHKILELGAGD
                     GMVSSSLQERGKQVTAIEFRDDDFDQRAHDSGVNLIKMDAAELQFEDHSFDFVFSYNC
                     FEHFPEPQKVFEESLRVLKPGGLMFIHFAPLYHSPYGAHAYNYVGIPYCQFLFDKHFL
                     NSWINTHANDKFSIDSTLNRWHIDQFRKLFKQTYWDRCKIGYYKEFRSPYFLDLVIKH
                     PQCFKNKIMSMDDLIINEIKAVFVKQ"
     gene            complement(29611..30006)
                     /locus_tag="PKY09_02270"
     CDS             complement(29611..30006)
                     /locus_tag="PKY09_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNB11708.1"
                     /translation="MIRHYTPALIWGLFIFILCAIPGKNLPHYSWADLLSVDKLVHFT
                     LFFILIILLKRAWLFVYNGVRSNNLLAIAIGVIYGGSLELMQSYFYTDRSGSWFDFLA
                     NSLGAICGAFFFGKLVDKDGRWKFSNANK"
     gene            complement(30016..30396)
                     /gene="gcvH"
                     /locus_tag="PKY09_02275"
     CDS             complement(30016..30396)
                     /gene="gcvH"
                     /locus_tag="PKY09_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNB11709.1"
                     /translation="MNLPADLKYTKEHEWIKIDGDMATIGITDFAQGELGDIVYVDIN
                     TLNESLKKDEVFGTVEAVKTVSDLFMPVDGTIVEINPKLTDAPDSVNKDPYGDGWMVK
                     VKISDASQLNALLTADGYAAIAGA"
     gene            complement(30459..31760)
                     /locus_tag="PKY09_02280"
     CDS             complement(30459..31760)
                     /locus_tag="PKY09_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11710.1"
                     /translation="MRKPKVLMLGWEFPPVINGGLGVACHDLCLAMVKHAKVSMVIPK
                     SNPDFVIKDLNLIGLNTIDSRTLKQVNYKEEYKTFEDVHFIPSALNPYYTDRKAAFEI
                     PGIDVESQYMKEIKIGNRHAKVFHIDDLYGGDVIEKVTVFGELAARLALTLDFDIIHA
                     HDWMTMLAGMEIKKQSGKPLVVHVHSLEVDRGGVNSQGWVYQMEKRGMQEADVLMPVS
                     NFTGNIINEYYGIDYRKIFPVHNGIRAVKPFRSKSPYKEKIVLFVGRLTRQKGPEYFV
                     DIAAKVLEKNKNVRFIMAGTGDAQNKILEKVAQNRIGHRFHLAGFLGLDKVRYLLSIA
                     DAYCMPSVSEPFGLSAVEAVQFDVPVVISKQSGVSEVLSGALKFDFWDINQAAEYLLG
                     LLDNDTLHNQVVKDAQKDLEDISWDIAAKKVIEGYKRYKLI"
     gene            32001..34040
                     /gene="paaZ"
                     /locus_tag="PKY09_02285"
     CDS             32001..34040
                     /gene="paaZ"
                     /locus_tag="PKY09_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HNB11711.1"
                     /translation="MRLKNYVAGEWVEGTGNGTPLFHAVTGEQIAIASSEGLDFKGML
                     EYARTVGGPALRAMTFHERSRKLKALAMYLMAKKEIFYQLSAATGATRTDSWIDIEGG
                     IGTLFAYASKGRRELPDETFLVEGNIEPLSKNGTFIGQHICVPLEGVAIHINAFNFPC
                     WGMLEKLAPTIVGGMPAIIKPATATSYLTELMVKEIIASGILPAGALQLICGSAGDIL
                     DHVNCQDVVTFTGSAETGRMLRQKPAIIDNSVRFTMEADSLNCCILGQDASPDTEEFK
                     LFIKEVAREMTAKSGQKCTAIRRTIVPAKYADEVATALKKRLTDIRLGNPSVEGVRMG
                     PLASKSQVKDVTAQVAKLMNVCEVAYGDLKNFEVTGADKEKGAFMPSMLLYCRKPLST
                     AEPHAIEPFGPVTTIMPYDNNDEAITLAKMGKGSLVGSLFTADDNTARQIVLNTAAYH
                     GRMLVINRHSAAESTGHGSPMPQLIHGGPGRAGGGEEMGGIRGVKHFMQRTALQGSPT
                     TLSNICNTYLKGAAITEDIIHPFRKYFDDLKIGDTLITHKRTITEADIVNFAGISGDN
                     FYAHTDETSLEGSIFERRVAHGYFIISAAAGLFVDPKKGPVLANYGLDNLRFTQPVYV
                     GDTIQVRLTCKSKTKKEDREGQVPQGVVEWDVEVTNQRAETVAVETVLTLVARKM"
     gene            34067..34942
                     /locus_tag="PKY09_02290"
     CDS             34067..34942
                     /locus_tag="PKY09_02290"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNB11712.1"
                     /translation="MKVLFTVWLCLAFSLTTFSQNEYNNFVVERKNVQKQIDEMLKSD
                     KAFKNKNATAEFQALIRKLLELDDKIILSANYTVNMLQNENKDLEKQANQKPKTIIKT
                     VHGKTVTVANDSIYDALFIAQNRLTNLNDALKAKDGAFDQLLNKSDSLIVANKTMQAR
                     FDALMADNKALSEKNIVLIVFNSLVAVGLILSLFFLLRKPVAKKMLLNTPDSEKPLAK
                     VEQSRVAQQVPVARPVEVKPEPVIKVSPEGILSASHDALDFKLDQIEKLARLKEKGYL
                     TDEEFNVQKRQILGV"
     gene            35169..36431
                     /gene="metK"
                     /locus_tag="PKY09_02295"
     CDS             35169..36431
                     /gene="metK"
                     /locus_tag="PKY09_02295"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNB11713.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAYDPTSKVACETL
                     VTTGQVVLAGEVKSNAYLDVQDIARKVIKEIGYTKSEYMFEAHSCGVFSAIHEQSTDI
                     NQGVERKKPEEQGAGDQGMMFGYACRETDTYMPLAIELAHLLLRELAAIRREGKVMKY
                     LRPDAKSQVTIEYSDDNKPQRIDAIVISTQHDDFAKEAAMQKQIKQDVINILIPRILK
                     KLPKRVQVLFNDKIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGVCDEVLVQVAYAIGVAQPVGLYVNTYGTAKVKM
                     TDGEISNKLSKMKEFDMRPYFIEKRFNLRSPIYLETAAYGHMGRESKTVEKVYNKGQA
                     NEKKIKVKLFPWEELNAVNETKKVFGIK"
     gene            complement(36496..37758)
                     /locus_tag="PKY09_02300"
     CDS             complement(36496..37758)
                     /locus_tag="PKY09_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNB11714.1"
                     /translation="MSNNTEPLKNNITIVGAGPAGATASMFLSSFRIKHTLIDKAVFP
                     RDKICGDALSGKVLPVLKKLNPDLIQQLLVQNENCLGSNGILFGAPNGKTLEVPFKAV
                     NSTINHSPGYVSKRMDFDYSLIKLLNPEYCNFLQNSEVKKITKGEEDVTLNVLDLQSK
                     ETYNISSSLVIACDGAHSICNKLFPDHKLDAGHYCGGVRAYYSGVKGFHRENFIELHF
                     LKEMLPGYFWIFPLPNGMANVGAGMLTRTISSKKVNLKEAMLFAIKNNPSIKDRFHDA
                     KIVGNIDGWGLPLGSKKRKLSGNRIILCGDAASMIDPFSGEGISNAMYCGMRAAEVAR
                     DAINSSNYANDFLSGYDQFIYNRLWNELKLSHTLQKLCNYPSLFNFVVNKASKNETFR
                     QTISCMFDDIDLRERLRKPSFYFKMLFG"
     gene            complement(37769..38908)
                     /gene="mqnE"
                     /locus_tag="PKY09_02305"
     CDS             complement(37769..38908)
                     /gene="mqnE"
                     /locus_tag="PKY09_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNB11715.1"
                     /translation="MTSDIILKSSVDPQLKMIAQKVVNAERITPEEGLALYEKGELGF
                     LGSLATLVRTRKNKNFTYFNKNIHIEPTNVCIFSCRFCSYSKDYKHREDGWELSIDEM
                     LDKVRAYPQGTITEVHVVGGVHPKMDLHYFAGLLKEIKNINPSLHIKAFTAVELEYMF
                     RKAKMTNAKGLQYLKEHGLDSIPGGGAEIFDEEIRNKICADKCNAEQWLNIHQEAHQL
                     NIPSNATMLYGHYESFAHRIDHMNRLRNLQDKTKGFQTFIPLKFRNKDNEMSHLDEST
                     QIEDLRNYAVARIFLDNFQHVKAYWPMIGRATAQLAQNFGVDDMDGTIDDSTRIYTMA
                     GSEEQNPTLSTQELVDLISQNGFIAVERDTLYNHLKVYENASVVN"
     gene            38999..39808
                     /locus_tag="PKY09_02310"
     CDS             38999..39808
                     /locus_tag="PKY09_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11716.1"
                     /translation="MQKIIAVCAAFLISVNCFAGEKDCHCTPLFKHGKGTFYLTWGYN
                     LDWFSKSDLHLKGNDFGGYDFTLYDAKAHDKPGLKYIFGSAISIPQYVYRAGYFFGDK
                     HDLGVEIAFDHTKYIMTNNQQLHLKGNINGTYYDQDTLVGKDFLMFEHTNGANFLMAN
                     LMKRYCLLSSASKNHRLQLILKAGGGLVIPRTDVTIFGNRLDNKFHIAGYVFGVDAGF
                     RYDLMKYFFVETSLKGAFANFTNVLTVGDGRAHHHFFAGEYIFAAGFQIGL"
     gene            39856..42213
                     /locus_tag="PKY09_02315"
     CDS             39856..42213
                     /locus_tag="PKY09_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNB11717.1"
                     /translation="MWKFLANKILRNRIAFIVVLLLLTVFMAYKALNIKLSYEFVRVL
                     PTTDPAYVEYENFKQKFGEDGSVMVIGFADSSIFQIEKFNSWFLLSDKIRKMDGIQNV
                     LSIGDLYDVVRNDSLHKFDFVSLVKSLPQRQSEIDSIKNRIDELPFYDGLILNRETHA
                     TVMAITFRKKDLDSKHRIDIVNEIKKEADAFGRLHNIELHYSGMPYIRTAVMEKVSHE
                     LKLFLLLAVLITAFILWVFFRTISSVVFSILVVAIGVVWSMGSIELFNFKITILTALI
                     PPLIMVIGVPNCIFLINKYHSEYARHGNRIKALARMISTVGISLFLANITTAIGFGVL
                     VFTNSSFLTQFGMVAAINVMVTYFITLVFIPIILSFLPPPSVRQMRHLEGKRMRKILE
                     WVDFIVHHKRNQIYVVIVSLTLLAVYGMTKIDVTGYVVDDLPRKNPVYTDMHFFEKNF
                     KGVLPFEIFIDTKKPNGVFDNNGRVLYKMKALQKQLENYPELSKALSITEALKFSYQA
                     YRGGDPKYYVLPGISELKNLNDYTSTVKGNENRLKSFIDSSRQYTRISMQMADVGSKK
                     IKTLLADIQPKVDSIFDSSQYHVKLTGHSLMFLKGNDYLLYNLYESLIIEILLITIVG
                     MALFRSIGIILLSKLPCLIPLVITAGIMGFFDIRFKPSTILIFSIAFGISSDGTIYFL
                     SKYRQELKQNKKSISEAISATIMDTGFSMIYTAVILFFGFSIFAASDFGGTAALGLLI
                     SITLLVAMVTNLILLPSLLLSIHKWVSRKEIISEPLIELDDNEES"
     gene            42308..43708
                     /locus_tag="PKY09_02320"
     CDS             42308..43708
                     /locus_tag="PKY09_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB11718.1"
                     /translation="MKSSSNAGALATIITVFFFWGFVAASNGIFIPFCKSHFNLSQFQ
                     SQLIDTAFYAAYFIGSLLLWFSSQLFRVDILNKLGYKQAVIAGLLISVAGSLLMIPAV
                     GSGSFSFILSAFFVIALGFSLQQTAAQPFVTALGTAETGAHRLNLGGGVNSFGTLLGP
                     ILVSLVLFGNLSPEAAASATVESVDTVYYILTGVFLLAALLLAVAKLPKVHSTEKFEP
                     GLGALKFSQLRWGMLAIFVYVGVEVTIQSNMGALLALPQFGGLSTAQISPYISLYWGS
                     LMIGRWTGAISAFNFKPTTKKLLMIVLPIVAYIIVLLANYLRGNEVISLLPYSLCVAA
                     LIAGSFLAQEKPARMLFIFSVGGILAMLIGLLTEGQVAMFAFISGGLCCSIGWPCIFA
                     LSITGLGKFTGQGSAFLIMMILGGALIPPLQGYLADVTDIHSSYWITVFCFAYLAWFA
                     IKVETILQKQGFHTNQ"
     assembly_gap    43806..43905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <43906..45138
                     /locus_tag="PKY09_02325"
     CDS             <43906..45138
                     /locus_tag="PKY09_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNB11719.1"
                     /translation="TAGGPGGWNPETHTFTDMADAIYRAYVRQIGAGAVAAGGFITLL
                     KTIPTIISSFKESVSSLKEGQGAKTVSRTENDLSFKTVIVGSVVLIILMALLPQVPGE
                     SFINKLLIGLLIVVFGFFFVTVSSRIVGIIGSSNNPISGMTIATIMGTALVFIAIGWT
                     GKVFEPMALVVGGLVCIAAANAGATSQDLKTGYIVGATPRNQQMALFIGAIVSSVVIG
                     FTVKYLDTPTADLTAQGIQHAIGEKYAAPQATLMATLIKGLLSHNLDWQFVLVGVFVA
                     ITMELCGVNALSFAVGAYLPLSTTLPIYAGGVVKGIVDWRAKRKHKKAEGDDELGKGN
                     LFATGLVAGGALAGVVVALLSVDEGIFAALEKINMHHNIANVIGENGYQLLGTVFFIA
                     MAIVLYRIAIKPEKKDIE"
     gene            45160..45684
                     /locus_tag="PKY09_02330"
     CDS             45160..45684
                     /locus_tag="PKY09_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNB11720.1"
                     /translation="MKKKLLGVVFSLFILTGYISCSQPSKEKIEPKIKWMDFESAVAA
                     SQKNPKKLFIDVYTDWCGWCKKMDASTFMDDSVAAYINANFYPVKFNAESKDTIRFFD
                     QVFVFVPDYKSHELAISLLNRQMGYPSYVFLDEKFASIKVQQGYMSVETLMPLLHYFA
                     KDAYKTKTFEEFTK"
     gene            complement(45925..46281)
                     /locus_tag="PKY09_02335"
     CDS             complement(45925..46281)
                     /locus_tag="PKY09_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNB11721.1"
                     /translation="MEQLLIAMEYNQGDIILLPYPYTDLSITKQRPAVIISKDSINKQ
                     NYIVAKITSVIRGDKFSFAIAPTDIDRELKYESEVRTNEVFTVSPTIIIKKFASFKKE
                     PLKRLTECVKDNISVE"
     gene            complement(46266..46697)
                     /locus_tag="PKY09_02340"
     CDS             complement(46266..46697)
                     /locus_tag="PKY09_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11722.1"
                     /translation="MVYSLTFTTNILESLGVLSSFNIAETIWEKYEKFAIPKIKDSTI
                     KLRGSLYYLKDLSPNEASKELNSISPIIPVLSKLRTVIEPIDNREFRDFKIAALDFFE
                     TVDFLYFNLQDIADVHSSYNLSKPVLATDWGSNEDEHWNNY"
     gene            complement(47017..48717)
                     /locus_tag="PKY09_02345"
     CDS             complement(47017..48717)
                     /locus_tag="PKY09_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11723.1"
                     /translation="MKKAILLLSIYFFATGLLNVSQAQSFQWLTRMGGPDSDLNFDPD
                     EWVRDIEVDADGNVYACGRIRSIANFGGVPVTTYGGYDMFLCKYDCHGNLVWVKTAGG
                     LDEDDAGDLVLDNIGHIYLTGRISSTPNRPCTFFDTTFTDVHIDMFLAKFDTSGNYLW
                     IKAAGTAAISGGSRGFKVLLDSNGQPVVSFGSSPGLLFPGWNTTEPNTAPYISRFDTS
                     GIMQSIFTFSTNYSVGAYCVTMDSQDNYILNSYFTEDSVLVGNQTIYNPKPGSYQALV
                     FKFSNSGIFDWSYMFTDTTIGLNGSLAFDCRIDANDNIFVTGLAYIGLKCGNFVFTDP
                     IAPSGASPFLIKLSPLGQPVWATQVYVGYSGKIDSAMPDNLTIAKEYRGLAIVGNDTL
                     QDSWVGAGNDDAFIARFDSNGNALNAVRLTNAGATEINAATKDNLNNIYIAGSFSNIL
                     TAGTHSVQSAGGHSDGFIAKYGYNCTTGMSPDLTAATVAGISVWPNPVAKGEAVTFAT
                     AITNGSYSVINMMGLEVAIGTFQKGKGTISTQALAAGVYTVVIQGQSNTGTARLVVVE
                     "
     gene            complement(48821..>51314)
                     /locus_tag="PKY09_02350"
     CDS             complement(48821..>51314)
                     /locus_tag="PKY09_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11724.1"
                     /translation="TFNASVFSTNPINSYQWLFDGGNIGNGPISNTIPVSPTGNHTVS
                     VTVTTSSGYTCTQNNTLNQLLIPTPPGAAFTIAPNPPFCEKQTVVNFNFTGSVTQVHH
                     ALWDFGDGASYAPLFPTSVQHIDAQRVYDAFSIPTYHVELKVYDNYGCYYTDDDYVSV
                     YENRLNGFPIPPLNSFCPGASKTLGYYPDVLQGGGNSIIPTDYLWSTGAVTPTLPVHS
                     TGQYYITVFDAASGCKYVSPRFADVSVYNVPNLIVRGKSDYCQGEAVFFDGYSGNGSY
                     EWQPLPGGLMLAPPSTNLQASAIHIAPPATGTYTVTLMLTTADGCSTLVSKTFNVLDP
                     PTVPLVAVQPTPGCENNAVTLTATNWAATLVLNWSQGGNTNPITVPNTGNYNAIFTDP
                     ASGCTSQSAIVRVNANPDISWLVSGCLDRCDNDQNHIVIPGSYGISYARWTWIINSQY
                     LNAPYSGYASPVGDLDINNALAGGGTLAPGSYIVQLELVSDMGCKITTDPIYVTVRTC
                     PCKITEVREDWYQCLGTDLNGQSFYHFQFTVHFNNLIPTGTFTLAPNPPGGNTNITPW
                     TYQVNGNDVMIEGILGFYQIDDILPCFTLTYTDPDPSLSCTYTFCVKPPDCPTPKCGW
                     EMEMDEIYCINLDANGNQTYAVNLNVSLPPGNWSAMYSLSSGTLTGFPASTTGGGITA
                     LQGIFTDLPPTNGQMCLYVNFYDVMTGDYCFVYKCFNLPDCNGGGTGNTRLTAALKEQ
                     HADEPQLTIMPNPASSQALVQFVLPAASGKDLNSVLTLTNARGAVINIRHIMANTGFV
                     RYNTSMLPEGMYYLTISTDGKTVLTQKFSVIK"
BASE COUNT        15898 a         9015 c         9738 g        16563 t
ORIGIN      
        1 attcttgcat acttgtctaa tttattttta tgttagttag tattcactcg caaaagtagg
       61 aactaatttt atactgcgca aataaaactc aaaaaaaaat tgatttattg tttactcatt
      121 cagagcacag gcaaaaaagt ggctcttttg actttactta cggtataatt gattataaat
      181 ggtgttcgtg attgctttgc agttaaaaat attttgtgcg atggaaaaaa attaaaacat
      241 agaacggtag gcaatgagtt cccgatagtt accgggaggc ttactcgttg gccacataca
      301 atatggtttg tcaatgcttt aaaactcaaa tttcagaacg attgttttag ttggaacggt
      361 cgtcctttca ttggttttca aactttcttc tgataatttg tgaggtaaat gccggtaaga
      421 attacaaaga gtgccagcag ataaagcata ttaaatgttt cattatcgta cagtccccaa
      481 agcactgcca caaatggaat caggtacgtt actgatgaag cataaagtgc agaagaaatt
      541 ctgattaaat ggttaaacaa aacggttgat attacagtac tcataatagc aagcaatgca
      601 atataaaaaa agctgttcca tgccaaaggt tctgtttgga aacgtgaaat aaaatcggta
      661 gaaaaaagat agatgccact tggtattcct gcaaaaagca atgctgcacc agttagcgta
      721 actgcattta ttgatgctaa tttatttctg agaatgttta cactaaatgc ataacatact
      781 gtggcaataa gaacataaac gcctttccag ggttctgcac tttgtgcatc ttctgcattg
      841 agccaaatca gtgaggctgc tcctgcaaag ccaataatca ctccaagtaa atgtctttta
      901 ctgaattggg ttgcaaaaaa taaactgcca actacaagcg taaacataga agttaatgaa
      961 ttcaacattc ctgccatgga accactgatg ccggtttctg catgagtaaa caggtaggcc
     1021 ggtatgccgt ttcctaataa gcctgttgcc ataacaaata accacagctt cttgggtatg
     1081 tcttttatgt agggtgcaat aaatggaagc atcaccatac atgaaataaa cattctgatg
     1141 gctgctacct gatttgcgga aaaactattg agacctcgtt tcattaatat gaaagaactg
     1201 ccccacatag atgcaagcac aagtaagtaa acccaacgca ttactttatt tcctgccagc
     1261 atgtctgcct tttctgcctg atttgttttt tcgatcgtga aaagcaccct taaattccgg
     1321 attgtctttt cttttctgac ggtcaatttc catctcacga agttttgttt cttcttttcc
     1381 gggctgaatt tgttcaacag aatccggtag gttcagtcgt ttaatagaaa tacctgcaag
     1441 cttttgaatt ttcttgatat gccattcatc ttgcggttca acaaacgtta cagaaaaccc
     1501 tgaggcaaat gcacgccctg tacgcccaat acgatgaatg taatcttcat aaactaatgg
     1561 tacatcaaaa ttaattacat gcgaaactcc gggcacatca atgccgcgtg cagctacatc
     1621 agtagctata agtattctaa tttgttcatc cctgaacctg ttaattgcat taactcttgt
     1681 ttgctgcgtt ttgtcaccat gcaaaacatt tatgccatct gcttcaaatt tcatcaacag
     1741 catttcggca acactgttag cgttcttttt tgacttacaa aaaacaatta ctttattaaa
     1801 attctctttg tctgtaagta aaaagtttaa cagatttaat tttgtctgaa aaccgtttac
     1861 cgtatatata aattgttcaa tttttgctgc agttttaact tgcggcacaa catcaataaa
     1921 aacaggaaac tccataaagt catccgatat tttttttact ctttcggaaa acgttgcaga
     1981 aaacagcatg ttttgtcttt ttcgtggtaa gacttcaagt aagctgtaaa tttgtccggt
     2041 aaaaccaaaa tccataagtc tttcggcttc gtccatcaca aagtgtttta tttttttcag
     2101 aacaagatga cctgaaagat aaagctccat tgcctgtgca ggagtggcaa caacaatatc
     2161 gcaacctgct tcaagttttt tcttctgatc agaaaaaccc ttgcctccat aaacggtcac
     2221 cactcttagg tcaacatatt tgcccatctg aattgctacg ttgccaattt gtatcgctaa
     2281 ctcacgggtt ggggcaagga tgagtgctcg cggttcatcc ccttgagcat aatttaaagt
     2341 acgcagcagc ggaagaataa atgcagctgt ttttcctgtt ccggtttgtg ctatcccaat
     2401 gatattctga cctgagagtg ctggaggaat taccttttgc tgaatttctg taggcgtttc
     2461 atagcctaag tctccaattg catttaataa ctggcggttt aatttcaatg aatcaaaacc
     2521 ggttgtggta ctcatttggg ttcgtcagcc tttttgcgat gactgaggct acgcattttg
     2581 atgttaagca tctctaccaa caacgaaaag cctaaagcca catatacata actcttgtcg
     2641 aagtgaatgt gtaaagcttc aacaacaagg ataactccaa taacaactaa aaatgccaaa
     2701 gcaagcattt ttaacgtagg atatttgttg ataaagtcac tcacataagg agcaaaggca
     2761 atcatcacca tcatagcacc aattacagca ccaaccataa ttactatctg attagaaaga
     2821 cctacagcag tgataattga atcgaaagaa aacacaacat ctattaccat tatctgaaaa
     2881 atagcttgta tcatcgtcat tttacgactt gggtttcccg atttatgagc ctctgcatct
     2941 ttaaatttat gataaatttc ctttaatgtt ttaataatta aaaacacacc tccggcaaac
     3001 aaaataatgt cgcgtccgct aactccaaaa cttccaatat aaaatagcgg attaatcatt
     3061 cttgcaatcc acgaaatggt aaacaacaat ccaatcctga aaaccaaagc cagtaataga
     3121 ccaataattc tggcacggct ttgctcttta cgatccggaa tgtaaccgca gataatagca
     3181 ataaaaataa tattatctat gcctaacaca atctccataa agagcagtgt tatcaaactc
     3241 acaattccgt ctgccgtcaa taaaggagca ataaattctt gcatatatta aattgaaggc
     3301 tgcaaaaata taaattgacg gttataattt ttaaataaac cagctgtttc aacaaaatta
     3361 aattttatgt gtttttaaaa ttcgttttta tctgaaataa cattactttg caaagtttta
     3421 attcaataaa cctacatgtg cggtattgcc ggtattgcat cttttactaa gagtgccaaa
     3481 gattttcagc aacatttgag gtctgcatcg tcatgtcttc gtcaacgagg accagacaat
     3541 gaaggttttt ttattgacga taatgtagct ctggcacata gaaggttgtc tataattgac
     3601 acatcggcag cagcaagtca gcctttttct gatgcatcag gtcgttacac cataatctat
     3661 aatggagagt tttttaatta tcgtcatcat aggtctcaat tggagtctca aggagtaagt
     3721 tttgtttccg aaagcgatac agaagtactt ttgcaattgt atatacgcaa gggcatcaat
     3781 tgcctgcagt gcattaatgg tttttttgct ttttgcattt atgataattt agagaagaca
     3841 gtttttttag cgagagacag aatgggcgtt aagcctttaa tttattatag tgataatgat
     3901 aaattcattt tcgcctcaga actaaaggca ttaatgcagt ttccgataga gcgaaatatt
     3961 gatcaacagt cattatatca ttattttcaa ttcaactata ttcctgaccc ttggacgatt
     4021 tatagcaatg taaataaact tagtccgggg aattatctgc ttattgacct taagaaaaat
     4081 tctgtaattg aagaagcttg ctattattca attccggaga ctgaaatgtt ggatatgccg
     4141 gactatcaat cggcacaatt gcgtcttaaa gagttaatga cagatgctgt agggttacga
     4201 ttagttagtg atgtacctct gggatgtttt ttaagtggtg gaatagattc ttccgtagta
     4261 acagctttag ctgcacaaca ggttaataaa cttaatacct tttcaatagg atttaaagac
     4321 gagccttttt ttgatgaaac atcatttgca ttgagggttg ctgaaatgca taacaccgca
     4381 catcatgttt ttaaacttac caatggtgag ctttttgaga atcttgcgga catattgaat
     4441 tatattgacg aaccttttgc tgattcttct gctattgctg tttttatcct tagtaaatac
     4501 accaaaaagc atgtaacagt agcactatca ggtgatggtg cagatgaaat ttttggcggt
     4561 tatcaaaaac acagagcaga gtggctaatc agaaataggc caggtttgac accattatta
     4621 aaaatggcgg cctctgtact tccttctaaa ttcagttcaa gagaaaactc tattaaaaac
     4681 aaaatcagac aattacatcg ctttgctgaa ggtgctactc taagtaacaa ggagcgttat
     4741 ttacgttggt gttcctttac aactgcaaac tatcggaatg agctgttact aaaaccattt
     4801 gccaatgacc taaaaaacag gaatcagcag ttatcaactt atgttaaaca tcaaacatca
     4861 atgaacgatg tgttgcttag tgatatgaaa cttgttttga caggggatat gcttcgaaaa
     4921 gttgacagta tgagtatggc taatagtctg gaggtacgtt caccattttt agattatagg
     4981 gtagttgatt ttgcattttc attaaccgac aagtataaaa ttgacaataa agctcaaaaa
     5041 aagattgtaa aagatgcttt cagattgctc ttgcccgaag aaatttatca tcgcggcaaa
     5101 agaggttttg aagtgccgtt aattaaatgg tttcagactg aactaagagg caaaattgat
     5161 tttcttttgg gagaagaaat tatacagagt cagggattat ttaatctgaa ttctattaaa
     5221 caattaagac atgatgtgat gaataaccca tccggggagt cacaggcaaa aatgtgggca
     5281 ttaattgtat ttcagaattg gtggcttaca catcatctta aatctaactg attatgccac
     5341 gtgtacttcg catcataaac agactcaacc ttggcggcat tacctataat gttacttatc
     5401 tcacccgttt catgcagcct gaatttgaaa cgatgctcgt ttcaggcgtt aagactgaag
     5461 acgaagaaag ttcggaattt attttagacc aatatggaat aaaaccgcat tatatcaggg
     5521 aaatgaaacg tgaaataaat ttctcttcag acagaaaggc ttatgctgaa cttaaatcac
     5581 tgataaaaaa atttaaacct gatattgttc atacgcatgc tgcaaaggct ggaacattgg
     5641 gtcgtttagc ggcattctca tgcaatgttc cggtagtggt acatactttt cacggacatg
     5701 tttttcattc ttatttcagt cctctaaaaa caaaattgtt tattgaaatt gaacggtatc
     5761 ttgccaaaaa aagttcagcc attgttacca taagcgaatt gcagaaacat gaaatctgta
     5821 atgttttcaa ggtaagtgca cctgaaaaaa cacatatcat tccattgggc tttgatctta
     5881 acaggtttag agaaaataaa gatgaaaaac ggatcgcgtt cagaaagatg tataagattg
     5941 ctgatgatgt agttgctgtg ggcattatcg gcaggtttgc tgccataaaa aatcatgctt
     6001 tctttcttga ggcgatgcaa tcagtaatca acaatacttc ccaaaaagtg attgctgttt
     6061 tgataggtga cggtgaagaa catgaatcta ttttagattg ttgtcgtaaa ctgaatatgc
     6121 catttgcaaa agatgcttca aacactgaaa caaaaattgt attgacatcc tggataaagg
     6181 atattgacag ggcattagcc ggtttggata ttgtagcatt aacctcgcat aatgaaggta
     6241 ctcctgttag tttgattgaa gcacaggctg ccggaaaagc agttgttagc acaagagttg
     6301 gaggtgtaga aaacgtggtt gctgaaggta gcaccggatt tttatgtaat aaaggagatt
     6361 tgcaatcgct tgtaaatcaa ctcctgcaac ttgtagaaga tatgggatta agaaataaaa
     6421 tggctgacaa tggttgggag catgttagaa ataaatttca ttatacccga ttggttaatg
     6481 atatgaaaga cctttatact gaattacttt cagccaaaca atagaatgat tactttgcta
     6541 aaaagattac ttttgcacag cgaaaaaaaa caccatccaa aaaaaatgaa gaatatcgga
     6601 aaattattgt tagtcttaat gttcggaagc cttatgacag gctgtactat caattccagc
     6661 ataatgttta agactaaaaa ggacttcgtc agccaaccgg acagaacaat tgggaatgta
     6721 gagtacaaaa ttgcacccaa tgacattttg agttgtagcg tttacaccaa tgatggtatt
     6781 aaattgattg accttacagc aaattcaaat tcgcttaatg aatctgcgaa tctaagtacg
     6841 gcaatgaatg gttcaaatca atatacagtt gatattgatg gctttgttaa gctgcccatt
     6901 attggaaaag ttaaaataca aaatctgact acacgtgagg cagaaaaact tcttgaggac
     6961 caatacgcaa cctattacaa caaacctttt gtgattataa aagtactaaa cagaagagtg
     7021 ttggtattcc ccggagaggg tggtgcagga aaggtggtaa ctttaactaa tgagaataca
     7081 acacttattg aaggacttgc tcttgcagga ggtatatcac aaaatggtat tgctaaaaaa
     7141 atcaagttaa taagaggcga caccagaaat ccacaagtta ctattattga tttgtcaaca
     7201 attgagggta tgaaagagtc taatctttta ttgcaggcta atgatatcat ttatgtggag
     7261 ccaagaaaaa gaatttcaca gggaatattg tctgaacttg caccaatcgt tgctatcttt
     7321 agtggaattg caagtatctt tattgcttat gacgtaatta aaagaaatca ataatcgctg
     7381 atgcctaaaa aacaaataac ccctctaaac gaggaatttg attttaagct ttttgtgacc
     7441 attgcccaac gcaatgttga gtggtttcta atgttcatga cactttccgt tatcatttgg
     7501 tatgtcatag ttagatatac agcacctatg tttgaatcat ctgccctgat gaagctaaac
     7561 acagataata atgtaaccaa agcactgaat atcactcgtg atgtttatca ggaacagaat
     7621 aacgaattgg caggtaatct ggaacttatc cgatcaagta taattgtcag taaagcaata
     7681 gccacactac cattgagagt cacatacatt tctaaaggta cgttactcga gaatgaaatg
     7741 tataagtcga gtccgtttga agtaaatttt ttaattaaag attcgttatg gttggacagg
     7801 cctttttatt tacaatttat caatgagaaa tcatttgtat tgtcatacat ccctgttggt
     7861 gaaaaagatc agattgaaga agaaaacgaa aacggaaaat ggattcattt aaaaggaata
     7921 tcattacaga ttaatgtaat caactatcag agtattttaa aacatcagtc acaacttaaa
     7981 aagaacactt ttaagtttat aatcaactca gataaagcac ttattaagga ttttagctct
     8041 aagctttccg tcatcatggc aaatcctgat gctaaaacta ttaatatcaa agtcaaagaa
     8101 aaaaatgcaa ctaaggcatc ggatatgtct aatgctatca gtcaggaatt tttgaaattt
     8161 gatgttttaa gaagtcgcga agcaactgac cgtattcttg attttattga tactacacta
     8221 tacagagtta ataacgattt gtctgaatct gaaataaatc tggaaagatt caaaagtcac
     8281 aaccgtatta taactcctga gttacttgca acagatttga gtagtaagat gagtcagatt
     8341 gaactcagat taacagatta cacattgaat cttcttgcca tttccgaact taagaaagca
     8401 attgaaagtg agaaaaaagt tgatgaaaaa atgctgagtt tagcaggaat ttacaagacc
     8461 gatgagataa aggaagcttt aaacagtctg aatgtattgc ttgataagcg agaagaagcc
     8521 ttaattcagg ttactgataa agcagaatat gttaagatgc ttaatgggcg catacaacgt
     8581 ttaaaagaat attttttaca atcagttact tacgaagaag agggcatcaa aacgaaaaaa
     8641 gcagagttag aatcacaata tagaaaatta aacagcaact atctgggtat tccggaacag
     8701 caaaccgagt ttgaacgctt gcagcgtata tttaatgtca atgaaaaatt ttattcatta
     8761 ttgcttgaaa agaaggcaga gttttcaata acacgtgcag gtttcgtttc aaagaatatt
     8821 atacttgagg cagcacgtcc tgcatctgcg ccatattatc ctaacaaaag cctgattcta
     8881 agttcatgta ttatggttgg ctttgtggta agttttttat tgattttcat aaaatatctg
     8941 ttttataatc aaattgggtc tcttgaagaa attgcgcaat atacagatgc acccttatta
     9001 ggaattgttc caagatataa aagagaaatt cctatttcac aattattggt tgataaaagt
     9061 ccaaaatctg ttatagcaga ggcatttcgt tctattcgca caaatcttca gtttatcaat
     9121 aatgatgaaa agccacaact gatggcatta acttcaacta tttcaggtga agggaaaacc
     9181 tttaatgcaa ttaatcttgc aggggtaatt tcattctcag gaaagaaggt gatcatcctc
     9241 gacttggata tgcgtaaacc taaaatacac cttgggttta atgtagagaa caacagagga
     9301 atgagtacaa ttctgattgg taaagacaca atagaaaatt gtatcaatca tagtgcactt
     9361 gaaaaccttg actttatcac tgcaggcccg attccaccaa atccgtcaga gttgattatt
     9421 agccctaaaa tggatgaatt gcttgagtat ttgaaattgc gttatgatgt aataattgca
     9481 gatacaccgc ctgttggtgt tgtgagtgat ggtattccaa tgattcaaaa agccgatttt
     9541 cctatttata ttcttcgtgc aaattatagt cgtaaaatgt ttattcataa catcaataaa
     9601 attatctctg aaaataaagt gccacgactt tcagtagttt taaatagtgt tgatcaaacc
     9661 aaactaaagt atggttatgg atacggttac ggctacggat atggttacgg atacggatac
     9721 agttatggct atggttacgg ttattattct gaagatgata aaaaaccaac cggcatctgg
     9781 aacagaatta ttagtttttt tatcaggaaa tagccttcaa tgaaaattat taaaactgct
     9841 ttaaacggca tttgtattat tcaggctgat gtattttcag atgagcgtgg atacttttta
     9901 gagtcatatc aaaaagagaa attgaaaaac cttggtattg aagctgaatt ttatcaggac
     9961 aacttttcat tgtctgccaa aaatgtattg cggggcttgc attttcaaaa tccaccattt
    10021 gatcagggta aactgataaa agtaatttgc ggggcagtgt tagatgttgt agttgatatt
    10081 cggaaatcgt ctcctactta tcaaaagaat tttactattg agttgaatgc agaagatcat
    10141 ctgatgatgt ggattccacc gggttttgcc catggctttg tttctttaaa agaaaacaca
    10201 cttttttatt ataaatgctc taaaccatat cataaagatt cagaagccgg aataatttat
    10261 aatgaccctg tattaaacat taattggagt gtaaatcagc cggttttatc agaaaaagat
    10321 aagattttac caaattttag taatgcaact attttgtttt aaatttctat actatacttt
    10381 tgctctaatt aagtataaca ataaccccac caactcatct catgcaggcg tagcacgaat
    10441 agttggttta attcaaacaa aatgaaagac attgtaaacc acaactatca tttatttatt
    10501 tatgccgtac tgtttttagg atcattggca ttttcatttt taattaactc attgtttttg
    10561 cgttttttct ttacgttagg catcagaaac accaatgatg gcactgttat ccgatggggc
    10621 tctatgtcta agcctgctgt aggtggctta tctttttata tcgtatttct attgtctttg
    10681 gccacctatt ctattttctt taataacaac gaaactttgt ataattctca atttatggga
    10741 ttcatcatgg caatggcaat tggttttcta attggactgg ccgatgatgc atacaatacc
    10801 aaacctgtat taaaattttt agctcaggtt tcatgcgcct tcagttttat ttttacaggc
    10861 acttacattg accttttcga taacatgatt ctcaattacc ttttaacaat tttctgggtt
    10921 gttggaatca tgaattccat caatatgctc gacaatatgg atggtattac ggcaacagta
    10981 agtcttggaa taattatttc tgctatggtg gttattacca tgagtaatga cattaataat
    11041 ctttatctgg cttccattat cggactatca gcaggacttt taggcttttt atattttaac
    11101 tggcatccct caaaaatgta tatgggcgac acaggcagtc agtttcttgg agtatttctt
    11161 gccggtatag gtatcatgtt tttctggaac aatccatatc atatcacaga gtcgcttcct
    11221 atcaaaaaaa ttcttactgt agcgattgca tttttacttc ctttagttga taccactgtt
    11281 gttgttgtaa atagagtcag taaaggtaag agcccattca taggaggtaa agatcataca
    11341 acgcatagtt tggcatattt gggattaaaa gacaatatgg ttagctgggt ttatgcagga
    11401 atttcattga tttcggtaat cttagtttat gttcttaatt ttctcataac tacctgggat
    11461 attttgtaca ccgtcatatt tgccggatat ttccttatcg ttctggctgt atttttttac
    11521 acaacatcac gcatcagagc tgcatccaag aaacctgttg aggcaactcc ggcatattct
    11581 gcatcgcact ataattaatc cggtctgctt ttttatttag ttcagggtaa gttatattct
    11641 gtaattttgt ggcgttacag atttcatgac aatttacatc atcattgcca acgttttatt
    11701 gagtttggtt tgttttagaa acaacgaatt gttgtctaaa atgatattta atccctatat
    11761 ggtatcacaa cacaaacagt ggtaccgatt tattacatct ggatttgtcc atgcagattt
    11821 tttgcatctt tttctgaata tgtttgtgct ctacagtttc ggccccatag ttgaaaatca
    11881 ttttgtactg attaatggtg aaagcggttc ggtattattt ctgatttatt acttaatggg
    11941 attagtggtt tcaattttgc caacatactt ccgcaacaaa gacaatcagt tctacaacag
    12001 tctgggtgca tcgggtagtg tgtctgcagt gctttttagt tttattttat tttttccatt
    12061 gcagaaaatt tatctctatg ggttgttagg aattccggga atccttgccg gagtaggtta
    12121 cttgtggtta tcgagttaca tggataaaaa aggtggagat aatatcaatc acaatgcaca
    12181 tttttggggt gctgtgtatg gttttgtttt tccaataaca tttgcacctg gattagtgct
    12241 ttctttcttt gatcaactaa caggtatgtt ctgatgatta attcaaacaa ggcaattttc
    12301 tttgaccggg atggagtaat caatgacaat tcactgtatt atacctataa aatagctgac
    12361 gttgtgttta atgacggcat ttttgaagct atgaaattat ttcaggacaa tggttatttg
    12421 atttttgtga ttaccaatca aagtggaatt gcaaagggtg tatattctca taatgatgtt
    12481 tgtgaagtac atagttttat gtcatctgaa tttaaaaacc gaaacataac aataacaaat
    12541 ttttattatt gcccgcacta tccggtgcag agtaaatgta tctgccgtaa gccagatagt
    12601 ctgttattag aaaaggccat tgcacgttat aagattgata aggataaaac tcattttatt
    12661 ggtgacatgg aaacagacat gatagcagca aaccgagcag gtgttatcgg tcatctgatt
    12721 gaatcaaaca gcaatattgt tgaattagca aaaaaaatct gtcaacacga tgtgggcgta
    12781 ttatgatgga caatgggttg attccacaac tcctgttttg aaattaaaca atagaggttt
    12841 tatttatggc gatggacttt ttgaatctat tcgttgtagt aggggtaaag caaaattttt
    12901 atcacttcat gttgagcgtt tacttgcagg tgctgctgtg cttagtctta actgtgattt
    12961 tataacagaa aaatttttaa atgaaattat ttttgaaatt aatcaaagaa ataaattttt
    13021 agctggcagt aaactaaggt tggtgcttac acgaaattca ggtgggacat acttgcctga
    13081 acagaataat acttcacttt gtttactccc atcagctgaa gtagaatctg aatatattgc
    13141 tcctgagaat tttgttgaac tcggagttta tagagaacaa caaaaaccaa ttaacaaact
    13201 ttcgggattt aaaaactgta atagcctcct ttatgtttta gcatcaatat attcgcaaga
    13261 aatgaaactt gatgatagtt tgatattaaa cagtaaaaat atgctttgcg aagcaacagc
    13321 atctaatgtg ttttggctga aaggcaatca ggtttataca acaccactat cttctggttg
    13381 tgtgaatggt gtgatgcgta aagtcattat tcaaatcacc aatgcaatag aacaggatat
    13441 ttcaattgaa caattagctg atgctgatga aatatttctt accaatacag gttcgggtat
    13501 taggccggta aaaaagatgc agggtaaggt gtatgaaact agttatggtg tgcaacttac
    13561 caataggctt aatgatttta ttatattatc aagatgattt tacaggctga caccatctgt
    13621 gctttaatta caccacccgg cagaggtgca gtaggtgtta tcagaatttc cggggcaaaa
    13681 acatttgaaa ttatttcaag aatatttatt cctgcaaata atagtttgtc aataccacaa
    13741 ttagaacatc aacgtattta ttttgggaaa attgttgaca ataatgatct gattgatgaa
    13801 gtacttgtct ccatatttaa agctcctgcc acatataccg gtgaagatgt tgccgaagta
    13861 agttgtcatg gttcaggtta cataagacaa agaatcttgc aacttattat gcaatctggt
    13921 gctgtgatgg caaagcccgg tgagtttacg atgagagctt acctgaatgg caaaatggac
    13981 cttgcacaag ccgaagctgt tgccgatatt attgaggcag agaatagcat ttcgcatcat
    14041 gcagctatgc aacaaatgcg gggtggatat tctaatgaaa taaaatctct tcgtgatcaa
    14101 ttaataaagt ttgcagccct cattgaactt gaacttgact ttagtgaaga agatgttgaa
    14161 tttgccagcc gtgcggaatt aaagacactt gttttaaatg ttcaacataa gatagaacat
    14221 ttgcttaaaa gttttgaatt gggtaatgtg ttgaaaaatg gagttcctgt tgtaattgcc
    14281 ggtaagccca atgtaggtaa gtcaacctta ttaaatgcat tgctcaatga agagcgtgca
    14341 atagtaagtg atattgcagg tacaacccgc gacacaattg aagaagagtt aaatgttgat
    14401 ggaatacgtt ttagatttat tgatacggca ggtattcgta agactgatga tgtaatagaa
    14461 agcattggcg tgaaaagaac ttttgccaaa gcaagcgagg caaaaattgt tttatatctt
    14521 tttgacccaa tggagtcatc accagccgac ttgcaaaatg aaattttgca cttgcagcat
    14581 gaactcaatc tttccgatat tgatttgaag aaaacaattt tgcttgttgc aaataaatcc
    14641 gatgctttga acgtaaactt tgatgttcac agttatgata aatgcggaaa tgtaatttta
    14701 atatcagcaa agaacaaatt aaaccttaat attttaaaag aaatattaat tgaacgctca
    14761 ggaattgatg gtgttgcttt gaatgagtct gttgttacca atgcaaggca tgcagcctcc
    14821 ttgtcacagg caaaggctgc tttagattta tttcttcacg gaatgaaaga aggaatcagt
    14881 aatgatttac tttcgacaca catcagagat gctatttatt ctttaggcga gataacagga
    14941 caagtgtcaa ctgaagattt gttggattat atttttagcc agttttgtat cggaaagtaa
    15001 ctatgtctca taaactttac ataaaaaata tggtttgcaa ccgctgtatt atggcagttc
    15061 agaagttgct gaaagatagt ggactcattg tgtcagatat tcagttaggt gaagccacga
    15121 ttgaaacaag acccactgaa gcagaaaaaa aacaattgca ggataattta aattatttag
    15181 gctttgaatt gattgatgat aagaaaagta gaattataga aaaaattaaa acagtaatta
    15241 ttgatttggt tcatcatcgt gatggtgagt taaaaatgaa tctttctgat gtgttgagtc
    15301 atgagttaaa tcatgattac tcttttttat ctaatatttt ttcagaagta gaaggaacaa
    15361 caattgaaaa gtattttatt gctcaaaaaa ttgaacgcgt taaagaatta ttggtttatg
    15421 atgaaatgtc attgagtgaa atttcatata aactgaatta ttcaagtgtt ggttacttga
    15481 gtaatcagtt taaaaaagta accggactta cacctagtca ttttaaacaa attaaatcca
    15541 ataaacgcaa gtcattagac aggatttaaa tattacaact aatatccaaa aaactgtaaa
    15601 ggtttttgat ttcattcttt gcacttttgc agtgtaaatt tgtttagata tggaaaagaa
    15661 tactgaagac actatttatc ttcctttaga gaatgtagaa agtgaacatt gcgcactgat
    15721 tgttgataaa gggctttcca aggttgcagg tgttcaatca cataaggttg aattgaataa
    15781 caggcgtgct gctattactg taaaaaataa agaatcttta ggtgatgcca ttgcaactat
    15841 taaaggtctc ggatatggtg taacaactgt caggagaaag tttcctgtat taaatatgtc
    15901 atgtgcttca tgtgcagtga gtgtagagag tatgattcaa tcggttaatg gtgtaattag
    15961 tgtttctgta aactttgcaa cagcagaact tacagttgaa taccttccca atatgaccaa
    16021 tccggtagca ttgcaaaagg tattgcagtc agttggatat gatttgttga taggcgatga
    16081 ggcatcacaa caaaatagta tagaaaacat acaacaggag aagtttaaaa agctaaaaca
    16141 gaagactgta ttggctattg tactgtcaat tcctgtggta gttattggga tgttttttat
    16201 ggatttatct tatgccaacg aaattatgtg gttattttcg acccctgtag ttctgtggtt
    16261 tggcaaagat ttttttatca atgcatggaa acaagcaaaa catcgttcag caaacatgga
    16321 cacacttgtt gcattaagta cgggaattgc ttatctgttc agtgtgttta atatgttgtt
    16381 tcctcaattc tggcatcaaa ggggacttca tgctcacgtt tattttgaag ctgctgctgt
    16441 gatagttgca tttattcttc ttggaaaaat gttagaagag aaagcaaagg gaaaaacatc
    16501 ttcagcaata aagaagttga tgggattaca gcctaaaact gttattgtaa ttcaatccga
    16561 tggaacagaa aaacaaactt ttgttgaaga tgtgactgtt gatgatatca tactcgtaaa
    16621 gcccggagag aaaattgcag ttgacggcac attaatcaag ggtagttcat acgttgatga
    16681 aagtatgctg acaggagagc ctgttcctgt tttgaaaaat ataaatgaca aagtatatgc
    16741 aggtacaatc aatcagaaag gcagttttca gtttaaagca ctgaagattg gaaaggaaac
    16801 tatgcttgct catatcataa aaacggttca ggatgcgcag ggtagtaagg ctccggtaca
    16861 aaaattagta gataaaattg caggaatatt tgtcccaata gttataggca ttgcaatact
    16921 aacctttgtt ctctggcaaa tttttggtgt tgataacgga atggtacagg gattgttggc
    16981 agcaatcaca gtattggtaa ttgcttgtcc atgtgcttta ggtcttgcaa caccaactgc
    17041 aattatggtt ggcgttggca aagctgctga gaatggcatt cttataaaag atgcagagag
    17101 tcttgaactg gcaaagaaag ttaccgctgt tgttttagat aaaaccggta caattactga
    17161 aggtaaagca gttgttacag atgtttattg gcataatgat aattcaggta ctgcatcaat
    17221 tctctatagc attgaaaaga agtctgagca tccgttagct gatgctgttg taaagaattt
    17281 ttctaagaca gaagttgtag aactttcttc atttgaaagt gttacaggta aaggagtgcg
    17341 agcaacatat ttgggagaga attattttgt tggaaatatg actttcataa aggaacagaa
    17401 tatttcaatt gatgatatgt tacttgaaaa agcaactgca tggactaaag aagcaaaaac
    17461 ggttatttgg tttactgaca gtgagcaagt gttatccata attgcaattt ccgataccat
    17521 aaagacaacg tctttgtcag ctattcagca attacatcaa atgggtattg aagtttatat
    17581 gcttaccggt gataacgaat caactgcaaa agcaattgct gaaaagactg gtatagataa
    17641 atttcaggca aatatgttgc cacaagataa agcagctttt ataaaagagt tacagcagca
    17701 aggaaaaatt gtggcaatgg ctggtgatgg aattaacgac agtacagcat tagcgacagc
    17761 agatgttagt attgcaatgg gtaagggtag tgatattgca atggatgttg caaaaatgac
    17821 tattgtttcc tcagatttaa ataagattcc aatggcgatt cgaatgtcaa aacaaacggt
    17881 agcaactatc aaacaaaatt tgttctgggc atttatttac aatgtcatcg ggataccaat
    17941 tgctgcaggc attttatatc cggtaaatgg atttttgtta aatccgatga ttgccggtgc
    18001 tgctatggca atgagtagtg taagtgtggt gagtaatagc ttgagattaa agttaaataa
    18061 gtataagtaa actaaataaa tgttgagata aaataatatt tagtacaggt ttaataacca
    18121 atttgcaaat ggtcaaataa ttaaattcag atagaatgga aaataagaaa cagtttcaat
    18181 tcaaaaccaa cattaactgt agtggttgtg ttgcagcagt aaaacctcat ttagataaac
    18241 taatcaacag cgagaattgg caagtagaaa caactaacaa agacaaaatt ttgtcggtta
    18301 taacaaatga ggtattagag gaagagatta ttaaaactgt acaaaaggct ggttttaaga
    18361 ttgaaatttt aaaaagtaat gatgtaggat gaaatttgtc ttattagctt tatgttattt
    18421 caataagaaa ttaaaatcac ctcaaaaaaa atacccacca aacatttcag tcaaatcaga
    18481 tgtttggtgg gtaaatgcta tcagttaaat tattcaacaa taaagtggcc tttcatggta
    18541 ccatagtgac caggaaaaga acaaagaaaa tcgtaagttc cttttgatgg agcagtaaat
    18601 tcaatttctg ttgtttctcc accaccaatt gttttagtat gtgcaattac actacttgcc
    18661 atgtcagcag gaatataatc gttgtctttt gctttaattg cagcagttgc aaaatcttgt
    18721 aatgatgtac ctgatgctaa aagtacaaaa ttgtgtccca ttgcctcttt aggtgatttt
    18781 cctgtatgct ttaaagttaa ttttacagtc tgaccttctt taactgtaat ttctgttttg
    18841 tcatatttca tgtcatcacc gcctgaaagc tcaactattg ccggctctgt tggtggtgct
    18901 acagctggtg cagcttcttc agttgcagca ggaggtgtag tttctgttgc aggcgtttca
    18961 ggttgttttg attgattaca tgctgcaaaa ataaaagcag ctgatgctaa tgctaaaatt
    19021 gattttctca ttttaatgta tttatttgat tgttatttat tgtaaaattg tttgtaattg
    19081 tatttaaatt aattgcgatt aaatctatgt tctaaaaaca ggtgcaaagg tatagcatca
    19141 attagttctt tgcaaaatac aagtattaaa ctaccgttaa ttatgaacag tttaaaaatg
    19201 agattttctt aagattcaag aatgctcaat agcaacttaa tgtgctcata attaaattga
    19261 tatgcaagtt gtttaaagta gtattttgga ataaatgatt ttgatctgtt tgaaaatttt
    19321 acataaaact gagaattatg ttttatcagt aatcctgttt ttaaacttga gtactaaaaa
    19381 tgtaatgtag gtaagtgcca taaagaaaat acctgttatt aaagccagca cacatgcacc
    19441 ggttgcatat tgcacaaaat ttgcatgaat agattgcaag gttatatcag tcatactttg
    19501 aaatttcaag tcgtttgaca caaaaaagcc tccgagataa taactaaaat aaatgattaa
    19561 agggatcatt ggtggaatac tgatattcgc agcagtaata aaaagtactt tatttaatct
    19621 aaacaaaact gccaagggaa taccaacaag caattgaaat ccccagattg gaactatgcc
    19681 cataaaaaca ccaaagccca ctgaaagtga ttttttaaaa atgctttcat cacttctgaa
    19741 aacctcatct tttattattg tgaaaatccc tttctttttg atatacttga ataaacgtaa
    19801 tggtagatga tagaaaagtg tcaacgttac taaataagta ttcaggatac tgattcttgt
    19861 aaaatcacga aaaggacgaa aatgtgatac acgttctttc atgtcgtata gtactctgat
    19921 atttacagga attattttta catctttcca tgcaagtttt actattactt caatttctgt
    19981 ttcaaattta gtcgtaaaca gtttgatttt ctttaatggt tcaagagggt acaaccgaaa
    20041 tcctgtttgt gtatcgggta atttttgtcc tgtctctacc caaaaccaaa agttagaaaa
    20101 tttattacca aacgaacttt ttccgggtac tgattcctga tccatatttc ttgaacccat
    20161 caataatgcc tcaggatact ttagggcttg tttaattatt acatcaatat cttcagggta
    20221 atgctgacca tcagaatcaa ttgttattgc atttttaaat cctctcctga ttgcttcttc
    20281 aaaaccttta cgtagtgaat aacctttgcc tttatttgtt ttgttggtta gtaaaataat
    20341 ttgttcatca tactgcttaa ggatttctgt tgtgtcatct gtagagccat cattgataac
    20401 aataacatca tttcccgaac tgaatatcaa aacaccattt attacctttt taagcgtttg
    20461 ggcattatta taagttggta taataacaca tgcagttatg ttcaccaatt tattcactga
    20521 tagaatgctg tcattttaaa aaaacagaca cccatatctt ctgcagatgt aactactttg
    20581 tataattgat catttttaga aatactgatt ttaaatgtaa cttcaggatt cttctccggg
    20641 ttcattacat ttaaaaattt gctgttaatg acttgcttca attttacatt ttgttcaatg
    20701 gaattctgtg tgagttctgt tgtcatctga attaatacca caccgggtgt aaccggttgt
    20761 cccggaaaat ggcctttaaa tatttcatgc ttagggttta gtttaatccg atactcacta
    20821 ttttgccctg atattttatt gatgatagtg tagaaatctt ttagcacatc agaagttttt
    20881 aaacaatgag tcgttgagtt cttggtttac catttgattg gagaacttca tgatatttct
    20941 atcacctgat ttttcataga aggctaattc ttcaagtaat aaattctttt tgttaaatga
    21001 aagttgtata taatcaaatg cattgctcat tcttttattt cttggcttta atttaacaat
    21061 atattctgtt ttagtttgaa agtactgcgg atcgaattgt ttgccattac taaaatcacc
    21121 attcactaaa gtaagcatca aatctttaat tcttccaaca acctgattgt atgtactgac
    21181 atctttttca tgattgtttt cctttactcg aactttatta ttttcactga taaaaacata
    21241 gcttgaaggt tttgtctttt cccaacgaag tttgttttct ttcttataat agaaaatacc
    21301 ataagatgtt tgaggctctt ttaatgctga cataaaacgc tgttcagtaa aatcagcttt
    21361 aattgaatta tttgaaatgc tggcttgctt gagcttttct gttaactcag ccggattgtt
    21421 taatggttcc agctgtgctt taactaaaac aggtatccag atgaatagta aaaatgtaat
    21481 tgagaatctc atgtttcgat taattctgct aacggtttaa tggcaatgtt attttgttta
    21541 caatagttta acaatgccgg taaaatgtca agtgtaatat cttttgtatc atgcaaaagt
    21601 acaattgctc cgggtttcaa ttgtgaagtt atccggtgta aaagcttttg ctcatttttt
    21661 gctgaagtgt caaaacttct gatactccat ccaatagatt tcatacctgt attcttcaac
    21721 acattttcat atctgggatt agttactcca aatggaggtc ttagatagac agttttttgt
    21781 ccggtaattt cttcaatttt ttggctgcat tgattaaagt ctgccaataa tttttgcttt
    21841 gaaaacatgc caattcctgc atgatgtgac catgaatggt tgcctatagt gtgacccttg
    21901 ctgacaatta gattaataat gtcaggatat tgttcagctt ttttgcctat taaaaaaaaa
    21961 gttgctttta cacctgccag ttcaagaaca tctaatattt tttttgtgac tttcggctca
    22021 ggcccatcat caaatgtcaa agaaatacct tcgggttttc ctgtattgac tgatttgata
    22081 aaatagttcc attttatttg ataggagccc aatgctgtaa ttgtcagata aaggataata
    22141 agtgttgcca ataaataaaa aaaagatttg tcctgataaa ggaaaacaaa taatgcaata
    22201 atgcagagga tgaaaagggc tgttattatt ttatgcttca ttacttttta tcattatgaa
    22261 cccggcacgt tcaggcagca aagaatttat tatcaaagcc cgttgatggc tatttatagt
    22321 cctgtcgtaa acctgtttgg ttttattgca aattatgtca gttgccagat gcaatgcaaa
    22381 ggcagaattt gttgcataca atccgcaaat ttcagaataa ttaatgatgc atttgccggg
    22441 taataaagct tcaattaatc ttgacacatc tttattgcag ccatcattta aaatgaagtc
    22501 aatttgttgc tcacctgttt tactcaagaa atcctttatt tcattttcaa gtgatgaata
    22561 aaaagcagta gtatgacatg cctcaataac tgctaaagtt ttttcgtttt tgttttttga
    22621 tattatataa aaagaagttc cgcttgtcag aggaaaatga tttaacttga aatatgctgc
    22681 aataggaagt agtggatcaa taacctcgtc agcactgcct ataagaatgt tcccatcatt
    22741 ttcatccagc attaacatgg catcaagcag tgcatgttca aatgacagac tgttttgcgt
    22801 gtgtgtcatg ttgtactgat ggttgccaag cactaatgaa atctgacctg caattgtgtt
    22861 atgggtagat tgaatgaatg aagtaggagg gagcagccca ccttcaatag taattgcatt
    22921 atttaaaaat ttttctgtgt cataaagaca acccaagcct gtacctgtaa taattgcatc
    22981 aggttgttta atgcctgcgt cttttaatac aagattactg caggcaacag acattctaag
    23041 tatctcactc atgcgtctga ttaatgaagg aggaacaaaa tctttataat cgggtttaat
    23101 taatgatcca atatctttag aaagtcggga ggaaaatcct atacttgcaa aggaaggctg
    23161 atgtgaaatg gtggcagcag attgaataca atacataatc aaattcttga aaaaatgagt
    23221 gaagaattat ttccaccaaa tccaaaagag tttgacatga catgttttgt taccgcctgt
    23281 gttggttctg taacaggacg cataagtgtc tcctctaatg ggtcattgta gtttaatccc
    23341 gaaaagtgta cttgattttt tatcgcaaga acagaatata ctgcttcgat agcaccggct
    23401 gccgccaaag tatgacctgt aaatgccttg gttgagctaa atggtgggat agcgttgcca
    23461 aaaacatttt taatagctat ggattcactc agatcattgt ttttagtacc tgtgccatgt
    23521 gcgttgatgt aagaaatttc ttttgactca aggcctgctt tattcaatgc atttttcatt
    23581 gcaatagttg ctcctttacc atcaggcgat gaagctgttt gatgataagc atctgcagca
    23641 ttatgccatc ctgttaaact gcaaattggt tcatttcctg aaatctttat gctgcgatca
    23701 ccttctaata ataaaaatcc tgcaccttca cctaagttaa gtccgtttct gttatgatca
    23761 aatggtttgc accattcatt atcatatatc atcaacgatt gaaagccttt aattgtaaaa
    23821 tcagttatcg gatcagttcc accaaccaaa actctatcca tttttccttg cgctatcatt
    23881 cgtgctgcaa gcataatggc attagctgct gatgaacatg ctgtagaaag tgtatttatg
    23941 taacctgaaa atttaaggta agcagcaatg ttttcagagg tatttccact gtcatgggtt
    24001 cgtgtcagat taaagtcaac aggtagattc tttaaaacct gtctgtaata tttttctgtt
    24061 tcatccatac cgccaacaga agtagatgaa atgaaagcgg taagtaattt tttattatgg
    24121 aagttgttac ccattgcttg ttctgctgca attattccga gtaatgaagt cctggaataa
    24181 tagccatctg taatagataa tagctgtttc aattctgaat ttgactttgc aatccttcct
    24241 gtgtaaatag atttttggtc atccggaaat ttacattcag ttataccgct ctttttattt
    24301 aaaagagaac gaaaattttc ttcaactgtc aaaccaattg cagaaactac acctatgccg
    24361 gtaacaaaaa ccttcatcga aatttacttt ttattcttct caatgtattc tgccatagta
    24421 ttgatggaat agaatacaga ttttccttct tcaggatttg tgagtttaat atgatagtgt
    24481 ttgtctaaaa gtactatcaa ctccaatgca tcaattgaat caagtcctaa gccactatca
    24541 ccaaataatg gagcttcatc catgatgtct gccggtgttt tatcctctag atttaattgt
    24601 gtaattaagt gtttctttaa ttcttctttt aaattatcca tttcacttta tgttattata
    24661 ccaatgttca atttctttac tatctaaaat ttcctgtgct gtttctgagt ttgattctgc
    24721 aaagaaaaca aatacatctg ttacaggaaa aaggttgacc catccacaaa gtaatgcttt
    24781 agctttttga ggaacaaaaa tgccggcttg agagctgaag tattcagcag aaaatgcagg
    24841 caataaggta aataaatttt caccatacca tttattcctt atcgccaact cacctaaaag
    24901 tatgttcgga agtgtatata caaattgagc aggagagggg cttttttcta ctttaaaaga
    24961 atgattaaat cgctcatctg attctgaaca agagtgactg ttagcaaata gcagtgcgat
    25021 ttcatcatca gcaaatttcg atattgcaga agttgtctga caaatgattt ctgttgccag
    25081 aaatcccatt tttgagagta aatccatttt ataaaactta ggatactctg ttttcagaaa
    25141 ccggtaaagc tgcattgaaa gctcgtcatg ctgactgttg tcagaggcaa aatatttttt
    25201 gccattaact aaaactttgt tttggtgaat tctgcaacag cctgatattt tcattttatc
    25261 ttttgtaata atagtgatgc attacatcca ccaaaaccgg atgctgtttt taagacagtg
    25321 tttattgtag cagaagcagc ttgtgttatt aaattaagct caatggatgt tcctttggta
    25381 taataaccgg cagaaggaag caatgtgttt tcatgcatac atgttatgca taaagctgtt
    25441 tcaattacac cggcagcacc aagtgtatgg cctgtaaatc ctttgaaact gtttaccgga
    25501 atgtgttgca tattgagcct gttaaaggct attgcttcca tttcatcatt aaatactgtt
    25561 gcagttccat gtgcagaaat gaaatctata ttttccgttt taatagctga tgcacttaac
    25621 gttttattta cacttcggaa taatccttct ccagttcttg aaggccctga aatatgattt
    25681 gcatcattac ttgaactgcc acttaaatag caaacaggcg ttgttgtaaa aacatcttta
    25741 tttgcagata acaaaaccgc accggcacct tcacctaaat taattccttt tctttcgctg
    25801 tcaaaaggtt tgcaaggctc atcacttata gcataaagag attgaaatcc ataaacaata
    25861 aaatcggtta tcatgtcaat accaagtaca attacatttt gatattgttt gttttcaata
    25921 aacgctgctg ctgcattaat ggccattaca cctgaagcac aggcattgga aatgctaaga
    25981 ggaaagtgtt ttaatttata tttttcatat agaaattttg ccggactgac aattgcattt
    26041 tcaaatcctt ttgataaatc acccttggta gtgctgatga taaggagcgt tgactcttca
    26101 gttaacagat ttgctttcga tttttctaat aactgttcaa ttgtttgtac agccaatgtg
    26161 ttaaaatcag gtctgctatc tgaaaacata gaacaataga acgattctcc attaaaacca
    26221 ttacctgtta ttttactaac acctgatttt ttctcggcaa tctttctgaa acaatctttg
    26281 gtattgccat taggactaag tatggtttcc gctcctatgt atatcacttg tcaatgttta
    26341 atgccaatgg acttttccca ttcaatgtaa aatggaggtt ttaccaattc catttcgcgt
    26401 tcaactacac ttaggaatac ctgcgtggta tgacctctgg cacttagttt tcctgtagtt
    26461 ttattcaaga tgtcaaaatc aaacatgatt tttgctgatg gtgtcgggtg gaattttgca
    26521 attgccacaa cttcttcacc ataattaatt ggcgatttat gttcaacaga tgatttgaca
    26581 attggtgtga aatagccacg atgataaaca tccagatagg tgagaccata ttttattccg
    26641 aatgcctcac gggcatcttc aaaaaagcgc agatagtttc catgccatac cacacccatt
    26701 gcatcggtat cgctgaatct tactttaatc gggatttcta cttgtaaaag ttgtttcatg
    26761 caagtgctat tttcatttca cattctatca gtaaattttc attacaagta tttcttcctt
    26821 ttattaaaaa aatattgcca agtgaattag tgatttcaat tttagttata atggttgtgc
    26881 cataatccgg caattgatgt acagtaagat tgctgataga acctatgtaa ccaatagatg
    26941 gaccttcgtt atttttctga ccaatgaatc caaaacctgc tgcacaggtt tgagcaatat
    27001 tttctatcaa tgcaggttct gttaaaaagc cgttttcgct aaaaatattt tcttccatta
    27061 cctgaaatga acttataaac ccggattcat cggcatgaat aagagagtca atcataataa
    27121 atggaaatcg ttgtggaata tagctttcga tattttcttt ctcaataatc attggctgta
    27181 aaaatcgtaa taattaaacc attgttcggg atattttttt actttttttt ccaattcttc
    27241 aacatacaat gctgcaatag cctcaggtga ttttttttct gtaatgggct cggtggcact
    27301 tagctcatag tgaaagtcgt tagctttcag agcaaaaaca aaagtgacgg gtgcactaaa
    27361 acgtgatgcc agtaaaaacg gaccataggg aaatttagct tttcctccca taaaatccaa
    27421 ttcaatagtt tttacacccg gcaaatacct gtcgccatgc atggcaatga gttcattagc
    27481 tgctaatgca ttatttactt ttacaatgtg tgataggtcg tctttcacag caattatgga
    27541 aaattgtgag ccaccggtgc tgttatccaa atattgtttt agtttttttg cttctgcatc
    27601 aaccattaat acgtgaatgg ttgacgaaac ccttgacttg agaaggtttc cggcagtttc
    27661 ccagttaccg acatgagcac tgatgagtat gccacctttt ttctgctctt gcatttcaat
    27721 caaatatttt tcgttttcaa aatgatgact gaatacattg ccttttccaa gcaaaaatgc
    27781 agtacgatca accaaggtct gccccaggaa gtaaaaattc tttctacagt aatttttagc
    27841 agcagcttcg tttaccttta aagctttccg ataaaagttt atgagtattt tctttggctt
    27901 tgctgcgaat ataaaatagt aataagtaac aaatcgcaaa aggaaataag cgggctttac
    27961 accaccatat tgaataagga aaacaaaaat gcgataacct aaaacagtgc ctctgctacg
    28021 gccgtcccac tgtgccattg tatcaggcca tggccgctag tttgcgctct acaaaattgt
    28081 agaaatcttc aaacgtgctg atacttttaa aatcttctcc ggaagtttta aaaccgaaat
    28141 tctcttcaat tacaacaaca agatcaacat aatcaagact gtccaattct aaagtatctt
    28201 tcagattttt ctgattctca attaaagttt catctacttc aaattcttcc gacaaaaaac
    28261 gttttgttgt tgttataatt tcttctctgt tcattcaatt ggtaaaatag gagggagtaa
    28321 tattttttgc aaagataatt tttgatgaag aaatatcaaa gcatttgtta aatcaagttg
    28381 catttttata cagttgttca tgtcatttct tcaatccatc tctacgattg acagagtgaa
    28441 atgttacgag tagcataata tttagtcatg atgcttttaa tgcgaattgt attgaagtct
    28501 aaattttata aacaaattca tttgtctgga actgaaagca ataacccaag ctaacatcag
    28561 tgagagtcaa acatcttgta aattaattgg attactattt atgacattga attctgtttt
    28621 tccttcgctt aaaacttatt aacaattcaa taataaaagt attacttatt ttacttttgg
    28681 acgcattaaa cctgaaccat agtgttagct aatattgcca aaagaatttt gcccgatggt
    28741 accagacagg tggtatctaa cttttactat gagcagaaat tgtttaaagc tgcccgggca
    28801 attgctatgg taaaaggacc ttcggaagta cttgatgaaa agcattttga gccattgttt
    28861 gaccagtttc ctgtagggga tcctgtactt tatgatgccg tttcaattaa agagagaggt
    28921 attcaacgtg ctgaggattt aattgggttt atgcataatt actccggagc atcttaccat
    28981 aaaatacttg aattgggtgc tggagatggc atggtaagtt catctttgca agagcgtggc
    29041 aaacaagtta cagccatcga attcagagat gatgattttg accagcgtgc tcatgattca
    29101 ggagtgaacc tgattaaaat ggatgctgcc gaacttcagt ttgaagacca ttcattcgac
    29161 tttgtttttt catataattg tttcgaacat tttcctgaac cacaaaaggt ttttgaagaa
    29221 tctctccgag tgcttaaacc gggcggctta atgtttattc actttgcgcc tctatatcac
    29281 tctccttatg gtgctcatgc ttataattat gtaggcattc cctattgtca gtttttattc
    29341 gataagcatt ttctgaatag ctggattaat acgcatgcca atgataaatt cagcattgat
    29401 tctacgctca accgatggca tatagaccag tttagaaagt tgtttaaaca gacctactgg
    29461 gatagatgta aaattggcta ttacaaagag tttcgttcac cttattttct ggacttagtg
    29521 ataaaacatc cgcaatgctt caaaaataaa ataatgagca tggatgattt aatcatcaat
    29581 gaaataaaag cagtctttgt aaaacagtaa ttacttgttt gcattactga acttccaacg
    29641 gccatcttta tcaactaatt ttccgaagaa aaatgcacca caaattgcac ccaaactgtt
    29701 ggctaaaaaa tcaaaccagc taccgcttct gtctgtataa aaataggatt gcatcaactc
    29761 taaacttcct ccataaataa ctcctattgc tattgctaaa aggttgtttg aacgcactcc
    29821 attataaaca aaaagccagg ctctttttaa gagtataatg agaataaaaa aaagtgtaaa
    29881 atgaaccaat ttgtccaccg ataataaatc cgcccatgaa taatgaggca aatttttacc
    29941 cggtatggcg caaagaataa agatgaataa accccatatt aaggctgggg tgtaatgacg
    30001 aatcattaaa atttatcagg cacctgctat ggcagcatag ccatcagcag taagaagtgc
    30061 gtttagctgt gaggcgtcag aaattttcac tttaaccatc cagccatcac cataagggtc
    30121 tttatttaca ctatccggtg catcggtaag ttttggattt atttcaacga tggtgccatc
    30181 tacaggcata aacaaatcag acacagtttt tacagcttca acggttccga aaacttcgtc
    30241 ttttttcagt gactcgttta atgtgttgat gtcaacataa acaatgtcac ctaactcgcc
    30301 ttgcgcaaag tcggtaattc caattgttgc catgtctcca tctattttaa tccactcatg
    30361 ttctttggtg tatttcaaat cagcaggtag gttcatattt attatttttt gttgaaatgt
    30421 ttttgcaaaa ttacttttta aaataaatct taaaaatatt aaatcagttt gtatcgtttg
    30481 taaccttcaa ttaccttttt ggcggcaata tcccaactga tatcttcaag gtctttttgt
    30541 gcatctttaa caacctgatt atgtaatgta tcgttatcca ataatccaag caagtattca
    30601 gcagcctgat tgatgtccca gaaatcaaat ttcaatgcac cggaaagcac ttcagataca
    30661 cccgattgtt tactgatgac gacaggcaca tcaaactgca ctgcttctac agcagaaagt
    30721 ccgaatggct cactcaccga gggcatacag taggcatcag caatagaaag caaataacgc
    30781 actttgtcta agccaagaaa tcctgcaaga tgaaagcgat gtcctattct gttctgagca
    30841 actttttcta atattttgtt ttgtgcatca cctgtaccgg ccatgataaa ccgtacattt
    30901 ttattttttt ctaaaacttt agctgcaata tctacaaaat attccgggcc tttttgcctt
    30961 gtcaaacgtc ctacaaaaag tactattttc tctttatatg ggcttttact tctgaaaggc
    31021 tttaccgctc tgatgccatt atgtacaggg aagatttttc tataatcaat accgtaatat
    31081 tcattgatga tatttccggt gaagttactt acaggcatta gcacatctgc ttcctgcata
    31141 cctctttttt ccatctgata tacccatcct tgactgttta caccgccacg gtcaacctca
    31201 agagaatgta catgcaccac caatggttta ccactttgtt tttttatttc cattcctgca
    31261 agcatggtca tccaatcgtg agcatggatt atatcaaaat ccaatgtcag ggctaaacgt
    31321 gctgcaagtt cgccaaaaac ggttaccttt tctattacat caccaccata aaggtcatca
    31381 atatgaaaaa cttttgcatg acgattacca atctttatct ctttcatgta ctgcgattcc
    31441 acatcaatac ccggaatttc gaaagctgct tttctgtctg tatagtatgg atttaaggca
    31501 gaaggaataa aatgtacatc ttcaaaagtt ttgtattcct ctttgtagtt aacttgttta
    31561 agcgttcttg agtcaatggt attcagccca ataaggttga ggtctttaat aacaaaatca
    31621 ggattgctct tagggataac cattgatact tttgcatgtt ttaccatagc cagacacagg
    31681 tcatgacagg caacgccaag tccaccgttt attacaggag ggaattccca tccaagcatt
    31741 aaaacttttg gtttccgcat aattgttaaa aatcagcttg aatactgatg aaaaaatgtt
    31801 ttgattttcc aacaggcaaa aatagggtaa agaatgattg gaaattattg aattattaat
    31861 gtttgattaa caatagttgc taagaataga atttaagggt tatgcacatg ttgttcttga
    31921 aattcaacag ccgataacct tgttacatgt ggttagattc tttaatttca cgccttcgaa
    31981 taaatatttt ttcaaagcag atgagattaa aaaattatgt tgccggagag tgggttgaag
    32041 gcaccggcaa cggaacacca ttgtttcatg cagttacagg agagcaaata gccatagctt
    32101 cttctgaggg attggatttt aaaggcatgt tagaatatgc acgaaccgtg ggcgggccgg
    32161 cactgcgtgc aatgactttt catgagaggt caaggaagct aaaggcctta gctatgtact
    32221 taatggctaa gaaagaaata ttttatcagc tgtcggctgc tacaggtgca acacgtacag
    32281 attcctggat tgatattgaa ggaggtattg gaaccttgtt tgcctatgcc agcaaaggca
    32341 gacgcgaact tcctgatgaa acattccttg tagaaggaaa tattgagccc ttatctaaaa
    32401 atggaacgtt tatcggacag catatttgtg ttcctcttga aggcgttgcc attcatatca
    32461 atgcatttaa cttcccatgt tggggaatgc ttgaaaaact ggcaccaaca attgtcggtg
    32521 gtatgcctgc aataatcaaa cctgctactg ctacttcata tctaacagaa ttgatggtaa
    32581 aagaaattat tgcatcaggc atattacctg ccggtgcatt gcagttgatt tgtggtagtg
    32641 caggagatat tttagaccat gttaactgtc aggatgtggt gacttttacc ggaagtgcag
    32701 aaacaggaag aatgttgcgt cagaagcccg ccatcattga taattcagtt cgttttacca
    32761 tggaggccga ctctttaaat tgctgtattt taggacagga tgcttctccc gatacagaag
    32821 aatttaaact ttttataaaa gaagttgcgc gtgagatgac tgcaaagtcc ggacagaaat
    32881 gtacagctat aagaagaacc attgttcctg caaaatatgc tgatgaggta gcgactgcac
    32941 ttaaaaagcg gctgacagat attcgtttag gtaacccttc tgtagaaggc gtacgaatgg
    33001 ggccgttggc aagcaaaagt caggttaagg atgtaacagc acaggtagct aaattgatga
    33061 atgtttgtga ggtagcttat ggtgatctta aaaattttga agtaaccggt gccgataaag
    33121 aaaaaggtgc ctttatgcct tcaatgcttt tgtattgcag aaagcctttg tcaaccgcag
    33181 agccacatgc catcgaaccc tttgggcctg taactaccat tatgccttat gataacaatg
    33241 atgaagcaat aacattagct aaaatgggta aaggaagcct tgtaggttct ttgtttaccg
    33301 ctgatgataa tactgccaga cagattgtat tgaatacggc agcctatcat ggcaggatgt
    33361 tggtcatcaa tcgccatagt gcagccgaaa gtacaggaca cggttctcca atgccacagc
    33421 ttattcatgg cggacccggc cgtgcaggtg gtggagagga gatgggaggt atcagaggcg
    33481 taaagcattt tatgcaacga acagcattac aaggttcacc tacaacactt tcaaacattt
    33541 gtaatacgta cttaaaaggt gcggctatta ctgaagatat tatccaccca ttcagaaagt
    33601 attttgatga tcttaaaatt ggcgacacat taatcacgca taaaagaacc attacagaag
    33661 ccgacattgt aaactttgcc ggaataagtg gtgataattt ttatgctcat actgatgaaa
    33721 catctcttga aggctcaata tttgaaagaa gggtagccca cggttatttc attatttcgg
    33781 cagcagcagg cttgtttgtt gacccaaaga aaggccctgt gcttgctaac tatggtttag
    33841 ataatttacg ttttacgcag cctgtttatg ttggtgatac cattcaggta agattaactt
    33901 gtaagtctaa aaccaaaaaa gaagaccgcg aaggccaggt gccacaaggt gtggttgaat
    33961 gggatgtaga agtaaccaat caacgtgcag aaactgtagc cgttgaaacg gttctgacat
    34021 tggtggcacg aaaaatgtaa ggtttatgga aaaaagatta aaattcatga aagttctttt
    34081 tacagtatgg ctgtgtttag cttttagtct gacaacattt tctcaaaacg aatacaataa
    34141 ttttgttgta gaacgcaaaa atgttcagaa gcaaattgat gaaatgctaa agtctgacaa
    34201 ggcatttaaa aacaaaaatg caacagcaga atttcaggca ttaatacgga aattacttga
    34261 acttgacgat aaaattattc tttctgcaaa ctatacggta aatatgttgc agaatgaaaa
    34321 caaggatctt gaaaaacaag ctaatcagaa accgaaaact atcataaaaa cggttcatgg
    34381 caaaacagtt accgttgcca acgacagtat ttatgatgca ctttttattg cacaaaacag
    34441 gttaaccaac ctcaatgatg ctttaaaagc taaggatggt gcatttgatc aactattaaa
    34501 caaatctgat agtctgattg tggctaataa aacgatgcag gccaggtttg atgctttaat
    34561 ggctgacaat aaagctttga gtgagaagaa tattgtgctt atagtattta actctttggt
    34621 agctgttgga cttattcttt ccttgttttt tctgctgaga aaacctgttg ccaaaaaaat
    34681 gttgttaaac acacctgata gcgaaaaacc tttagcaaaa gttgaacaga gtagagtagc
    34741 gcagcaagta cctgttgcaa gacctgttga agtaaaaccc gagcctgtaa taaaagtttc
    34801 acccgaaggc atattgtctg cctcgcatga tgcattggat tttaagctcg atcagataga
    34861 gaaacttgcc cgcctgaaag agaagggcta tctgacagac gaggaattta atgttcagaa
    34921 acgtcaaata ctgggggttt gattgttttt gttttcaggc acttttgctg aactaaaaaa
    34981 ctgctgtagg atttaataac aagttgattg ttttttagtg aatgacatta gtcatacgga
    35041 actttaattg atgattaaaa gtgtgtaact tttatgtttt gttaataata ctgaaaaggt
    35101 attgaacaat ttgatttttg gtttgataaa tatggttatt tttgcagcct taaatcaaaa
    35161 tttcaatcat gccatattta tttacttcag agtcggtatc agaaggacat cctgataaag
    35221 tagccgatca gatttcagac gctttaattg ataatttttt agcatacgac ccaacgtcaa
    35281 aagttgcttg cgaaacattg gtaactacag gtcaggttgt gcttgcaggc gaggtaaagt
    35341 ctaatgctta tttagatgtt caggatattg cccgtaaggt aatcaaagaa attggttata
    35401 caaagagtga atatatgttt gaagcacatt catgcggtgt gttttctgct attcacgaac
    35461 aaagcacaga catcaatcag ggagtagagc gcaaaaagcc cgaagaacaa ggtgccggtg
    35521 atcagggaat gatgtttgga tatgcctgcc gcgagactga cacctacatg cctttagcaa
    35581 ttgaattagc acatttatta cttcgtgaac ttgctgctat tcgcagagaa ggcaaagtga
    35641 tgaaatatct tcgtcctgat gcaaaatcac aggtaactat tgaatacagc gatgataaca
    35701 agccacaaag aattgatgca attgttattt ctacgcagca tgatgacttt gcaaaagagg
    35761 ctgccatgca aaaacaaatc aaacaagatg taatcaatat tttaattccg cgtattctta
    35821 aaaaactccc caagcgcgtt caggtgttgt ttaatgataa aattaaatac cacattaacc
    35881 caacaggtaa gttcgtaatt ggcggaccac atggcgatac aggactaaca ggaagaaaaa
    35941 ttattgtaga tacttatggt ggcaaaggtg cacacggtgg tggtgctttc agtggaaaag
    36001 acccttcaaa agttgaccgc agtgccgctt acgcaacacg acatattgca aaaaatcttg
    36061 tagctgcagg cgtatgtgat gaagtgttgg ttcaggtagc ttatgcaatt ggtgtagcac
    36121 agcctgttgg tttgtatgta aacacttatg gcacagctaa agtgaaaatg actgatggtg
    36181 aaatatccaa taaactttca aagatgaaag agtttgatat gcgtccatat tttattgaga
    36241 aacgttttaa tcttcgttcc ccaatctatt tggaaacagc agcctatggt catatgggtc
    36301 gcgaatctaa aacggttgaa aaagtttaca ataaaggtca ggcgaatgag aaaaagatta
    36361 aagtaaaact ttttccatgg gaagaattaa atgcagttaa cgaaaccaag aaagtttttg
    36421 gaatcaaata aaaaaagccc cgcaaggggc ttttttattg atgtttattt aaaacagaaa
    36481 taacaataat tgttatcagc caaaaagcat tttaaaataa aaggacggct ttcgtaatct
    36541 ttctcttaaa tcaatatcat caaacatgca gcttattgtc tgtcggaagg tttcgttttt
    36601 tgatgcttta ttcactacaa aattaaacag tgaaggatag ttgcaaagct tctgaagtgt
    36661 atgactcaat tttaattcat tccaaagtct gttataaata aactgatcat agcctgataa
    36721 aaaatcatta gcgtagttgc tgctgtttat tgcatcacga gctacttctg ctgcacgcat
    36781 gccacaatac atggcattac tgatgccctc accgctgaat gggtcaatca tactcgctgc
    36841 atcaccacat aaaataattc tgttgcctga taattttctt ttcttagaac ctaaaggcaa
    36901 tccccaacca tcaatgttgc caacaatttt tgcatcatga aagcgatctt ttattgacgg
    36961 attgttttta atggcaaaaa gcatggcttc tttcagattt actttttttg aactgatggt
    37021 tcgtgtaagc atacctgcac caacattagc catgccatta ggtaatggaa atatccagaa
    37081 gtatcccggc agcatttctt tcagaaaatg caattctata aagttctctc tgtgaaatcc
    37141 ttttacgcct gaatagtatg cccgcacgcc tccgcaatag tgccctgcat cgagtttatg
    37201 atcaggaaac aatttattgc aaatgctatg tgcaccatca catgcgatga ctaaagatga
    37261 acttatattg taagtttcct tactctgtaa atctaaaaca ttcaatgtaa catcttcttc
    37321 gccttttgtg attttcttta cttcactatt ttgcagaaaa ttacaatact cggggttcaa
    37381 taatttaatc agtgagtaat caaaatccat tcgtttgctc acatatcccg gtgaatgatt
    37441 tatagttgag tttacggcct taaacggaac ttccagtgtt ttaccatttg gagcaccaaa
    37501 caatattcca ttgcttccca gacagttttc gttttgtact aatagctgct gaatcaaatc
    37561 cggattgagt tttttcagaa caggtaaaac ttttccgctt aaagcatcac cacaaatttt
    37621 gtcacgtgga aaaactgctt tatcaatcag tgtatgtttg atgcgaaacg atgaaagaaa
    37681 catggatgca gttgcacctg caggaccagc acctactata gtaatgttgt tttttaacgg
    37741 ctcagtatta ttcgacacaa caattctatc aattaactac tgaagcattt tcatatactt
    37801 tcaaatgatt gtaaagcgta tcgcgttcaa cagctataaa accattctga gaaatcaaat
    37861 caactaattc ctgtgtggat aatgttggat tttgctcctc tgaacctgcc atagtgtaaa
    37921 tacgtgttga gtcatcaatt gtgccatcca tatcgtcaac accaaaattt tgtgcaagct
    37981 gcgctgtagc tctgccaatc ataggccaat atgcttttac atgctgaaag ttatctaaaa
    38041 aaatacgggc aacagcatag tttcttaaat cctctatctg tgtgctttca tcaagatgac
    38101 tcatctcatt atccttattc ctgaatttta aaggaataaa agtctgaaac ccctttgttt
    38161 tatcttgtag atttcttaat ctgttcatgt ggtcaatgcg atgtgcaaag ctttcataat
    38221 ggccatacag cattgttgca tttgaaggta tgtttaattg atgggcttcc tgatgaatat
    38281 tcaaccactg ctcagcatta catttatctg cacatatttt attgcgaatt tcctcatcaa
    38341 atatttctgc gccacctccg ggaatactat ctaatccatg ttctttcaga tattgaagtc
    38401 cttttgcatt tgtcattttg gccttgcgaa acatgtactc caattcaaca gcagtaaatg
    38461 ctttgatatg cagagaagga ttgatgtttt ttatttcctt caacaaacct gcaaaataat
    38521 gtaaatccat tttcggatgt acaccaccaa ccacatgcac ttcagttatt gttccttgcg
    38581 gataagccct tactttatcg agcatttcat caatactaag ttcccagcca tcttcgcggt
    38641 gcttgtagtc cttactatag ctgcaaaacc tgcaagaaaa aatacaaacg tttgtgggct
    38701 caatatgtat attcttatta aagtaagtga aatttttgtt tttacgggtt cttactaagg
    38761 ttgccaaact gcctaaaaaa ccaagttcgc ctttttcgta aagcgctaat ccttcttccg
    38821 gtgtaattct ctctgcatta acaactttct gtgcaatcat tttcaactgc gggtcaacag
    38881 atgattttaa aattatatcg ctcgtcatac tcaaatccaa atattatgca aagaaacata
    38941 aaataaagaa aatggttttg attttcatgt agctttgcgc catttatttg ataataatat
    39001 gcaaaaaatt attgctgttt gtgctgcatt cctgatttct gtaaattgtt ttgccggtga
    39061 aaaagattgt cattgcactc ctttatttaa acatggaaaa ggaacctttt atcttacttg
    39121 gggctacaat ctcgattggt tttcaaaaag cgacttacat ttaaagggta atgattttgg
    39181 tggttatgat tttacattgt acgatgcaaa agcgcatgat aagcccgggt tgaaatatat
    39241 tttcggttca gccatttcta ttccgcaata tgtgtatcgt gccggatatt tttttggcga
    39301 taaacatgat ttaggtgtag agattgcatt tgatcatact aaatatatta tgaccaacaa
    39361 tcagcagtta catcttaaag gcaacatcaa tggtacatat tatgatcagg acaccttggt
    39421 tggtaaagac ttcctgatgt ttgaacatac taacggtgca aattttttaa tggccaactt
    39481 gatgaagcgc tattgtcttc tttcttctgc ttcaaaaaac cacagattac aattgatttt
    39541 aaaagccggt ggtggtctgg taatcccacg aactgatgta acaatatttg gtaacaggtt
    39601 agacaataaa tttcatattg caggctatgt ttttggtgtt gatgccggat ttcgttacga
    39661 cctgatgaaa tatttttttg ttgaaacaag tttaaaaggt gctttcgcca actttacaaa
    39721 tgttttaact gttggtgatg gacgtgcaca tcatcatttt tttgcaggtg aatatatttt
    39781 tgctgcagga tttcaaatcg gactgtaata gtactttagt cagtaaaagt tggtttacct
    39841 atctttgtcc cattcatgtg gaagttttta gcgaataaaa ttttaagaaa tcgcattgca
    39901 tttattgtag tgctcctttt actgactgta tttatggcct ataaggcttt aaatattaag
    39961 ttgtcctacg aatttgtaag agtgttgccc acaaccgacc ctgcttatgt agagtatgaa
    40021 aattttaaac agaagttcgg agaagatggt agtgttatgg tcattggttt tgctgacagt
    40081 agcatatttc aaatagaaaa gttcaacagt tggtttctgc tttcagacaa aatcagaaaa
    40141 atggatggca ttcaaaatgt actttctata ggtgatttgt atgatgttgt cagaaatgat
    40201 tcgctacata agtttgattt tgtctcctta gttaaatcct taccacaacg tcaatcagaa
    40261 attgatagca ttaaaaaccg aattgacgaa ctgccatttt atgatggact gattttgaat
    40321 cgtgaaacac atgctacggt gatggccatc acttttcgta aaaaagatct tgacagcaaa
    40381 catcgtattg atattgttaa tgagataaaa aaggaagctg atgcctttgg tcgtttacat
    40441 aacattgaat tgcattattc cggaatgccc tacatacgca ctgccgttat ggagaaggtg
    40501 tcgcatgagt tgaaattatt tttgttactg gcagtgttga taactgcatt tattctttgg
    40561 gtgtttttcc gaaccatttc atccgtagtg ttttcaattt tggtggtagc cattggtgtg
    40621 gtttggagta tgggaagtat tgaattgttt aatttcaaaa tcaccattct gactgcgtta
    40681 attccaccac tgattatggt cattggtgtg cccaattgta ttttcctgat caataaatat
    40741 catagtgaat atgcaaggca tggcaatcgt atcaaagcat tagcgcgaat gatttcaacg
    40801 gtaggaatct cacttttttt agccaacata accacagcaa ttggatttgg tgtgctggtt
    40861 tttaccaaca gttcatttct cacacagttt ggaatggttg ccgccatcaa tgtgatggta
    40921 acttatttta ttacccttgt ctttattccc attattctta gttttttacc accaccttcc
    40981 gtgcgtcaga tgcgacattt ggaaggcaaa agaatgcgta agattttaga gtgggttgat
    41041 tttattgttc atcataagag gaatcagatt tatgtagtaa tagtttcgtt gacactgctt
    41101 gctgtttatg gaatgacaaa gattgatgtc accggatatg tggtagatga tttgcctcga
    41161 aaaaatcctg tttacaccga catgcatttt ttcgaaaaga attttaaagg agtattgcca
    41221 tttgagattt ttattgacac aaaaaaacct aatggcgtat ttgacaataa tggtcgcgtt
    41281 ctttataaaa tgaaagcttt gcagaagcaa ttggaaaact atccggagtt aagcaaagca
    41341 ttatctataa ccgaagcatt aaagttcagc tatcaggctt atcgtggtgg cgatcctaaa
    41401 tattatgttt tgcccggcat cagtgagctt aaaaatctga atgattatac ctctaccgtt
    41461 aaaggaaatg agaacagatt gaaatctttt attgattcat caagacaata tacacgcatc
    41521 agtatgcaaa tggctgatgt gggttctaaa aaaattaaaa cactgctggc cgacattcaa
    41581 cccaaagttg attcaatttt tgatagctca caatatcatg taaaacttac aggacatagc
    41641 ctgatgtttc taaaaggtaa cgactatctt ttgtacaacc tttatgaaag tctgataatt
    41701 gaaatattgc tgattactat tgttggaatg gcattgttcc gttctattgg aattattctt
    41761 ttaagtaagt taccttgcct tattccgctg gttataacag caggaataat gggttttttt
    41821 gatatccgct tcaagccttc tacaatactt atttttagta ttgcctttgg tatttcaagc
    41881 gatggcacaa tttatttttt atccaaatac aggcaagaat taaagcagaa taaaaaatct
    41941 attagcgaag caatctctgc tacaattatg gacacaggat tttcaatgat ttatactgcc
    42001 gtaattctgt ttttcggatt cagtattttt gctgcttccg attttggtgg cactgctgca
    42061 ttaggactgc ttatttcaat tactttgtta gttgccatgg tcactaattt gattttatta
    42121 ccatcgctct tactctcaat ccacaaatgg gtcagccgta aagaaattat cagcgagcca
    42181 ctcattgagc tcgatgataa tgaagagagt taagcttttt gttaacatgc tttagcggaa
    42241 aacttccttt ttgttagaga ttgcaataat tttttatgct gatgtttgga atatcaatca
    42301 tcatcaaatg aaatcatctt ccaatgcagg ggcattagca actatcataa ctgttttttt
    42361 cttttgggga tttgtggcag catcaaatgg catctttatt ccattttgta agtcgcactt
    42421 taacctgtca caatttcagt ctcagttaat tgatacggca ttctatgcag cctattttat
    42481 cggctcatta ttattgtggt tcagctccca attattccgt gttgatattc tcaataagtt
    42541 aggatataaa caagctgtca ttgcaggatt gttaatttct gttgcaggct ctctgttgat
    42601 gattcctgct gtaggttccg gatcttttag ctttattctt tcagcatttt ttgtaattgc
    42661 attaggattc tctcttcagc aaactgctgc acaacctttt gttacagcat taggcactgc
    42721 cgaaacaggc gcacacagac taaaccttgg tggtggtgta aactcttttg gaacattgct
    42781 tggtccaata ttagtcagtc tcgtattgtt tggcaacctt agccccgaag ctgctgccag
    42841 tgcaacagta gaatctgttg atacagttta ctacatactg acaggtgttt ttttactggc
    42901 agctttattg cttgctgttg ctaaacttcc aaaggtgcac agtacagaaa aatttgaacc
    42961 cggcctgggt gctttaaaat tttctcaact gcgatggggt atgcttgcca tttttgttta
    43021 cgttggagta gaggtaacca ttcaaagcaa catgggtgcg ttactggcat tgccgcagtt
    43081 tggaggactc tcaacagcac aaatatcacc ttacatttct ctttactggg gtagtttaat
    43141 gattggtcgc tggacaggtg ccatcagtgc ttttaatttt aagccaacaa ctaaaaagct
    43201 gctcatgatt gttctgccga tagttgctta tatcattgtt ttattggcca attatcttcg
    43261 tggcaatgaa gttatcagtc tgttacccta tagtctttgt gttgctgcac tcattgcagg
    43321 aagtttttta gcgcaggaaa agcctgcacg tatgttgttt attttttctg ttggcggtat
    43381 tctggcaatg ctaatcggac ttcttacaga aggacaggtt gccatgtttg cttttatcag
    43441 tggtggcttg tgctgtagca tcggatggcc ttgtattttt gctttaagca taacagggtt
    43501 gggtaaattt accggacaag gctctgcttt tctgattatg atgattcttg gaggggcatt
    43561 aattcctcct ttacaaggtt atttagctga tgtaactgat attcactcca gctactggat
    43621 tactgtattt tgctttgcat acttagcttg gtttgccata aaggttgaaa ccattttaca
    43681 aaaacaagga tttcatacca atcaataaag accttaacat ttttttattt tggctaatta
    43741 aaatatcctt tacattagcc atctcacttt ttatttatgc aaccacaaaa agagtttaag
    43801 ccatannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactgc aggtggaccc
    43921 ggtggttgga atcctgaaac tcacactttc accgatatgg ccgatgccat ttatcgagcc
    43981 tatgtaagac agataggtgc aggtgctgtg gctgccggtg gatttattac cttactcaaa
    44041 acaataccta ccattatttc atcttttaaa gaaagtgtga gctctctcaa agaaggacaa
    44101 ggtgccaaaa cagtttcaag gactgaaaat gatttatcat tcaaaacagt tattgtaggt
    44161 tcagttgttt taatcattct tatggcatta ttaccacagg tgcccggtga atcttttatc
    44221 aataaattac tcatcggatt actaatagtt gtgtttggct ttttctttgt aacagtttca
    44281 agccgcattg tcggtatcat tggtagcagt aataatccta tctcaggaat gaccattgcc
    44341 accatcatgg gtactgcttt agtttttatt gctatcggct ggacaggtaa ggtttttgaa
    44401 ccgatggctc ttgttgttgg tgggctggtt tgtattgccg ctgccaatgc cggtgcaact
    44461 tcgcaagatt taaaaaccgg ttatattgtg ggtgccacac cacgcaatca acaaatggca
    44521 ctttttatcg gggcaatagt atcatctgta gttatcggct ttaccgtaaa atatctcgac
    44581 acacctactg ccgatttaac agcacaggga atacaacatg ccattggcga aaagtatgct
    44641 gcacctcagg ctaccttaat ggcaacttta attaaaggtt tgttgagcca caacctcgac
    44701 tggcagtttg ttttggttgg cgtttttgtg gctatcacta tggagctttg tggagtcaat
    44761 gcattatcct ttgccgttgg cgcctatctt cctttaagca ccacgttgcc catttacgca
    44821 ggtggcgttg taaaaggtat tgttgactgg agagcaaaac gtaaacataa aaaagccgaa
    44881 ggtgatgatg aattaggcaa aggaaatttg tttgcaacag gtttggttgc aggtggagcc
    44941 ttggcaggag tagtagtagc acttctttca gttgatgaag gtatatttgc agctctcgaa
    45001 aaaataaaca tgcatcacaa tattgccaat gttattggcg aaaacggcta tcaattgctt
    45061 ggaactgtct tttttattgc tatggccatt gtgctttacc gaattgcaat taaacccgaa
    45121 aaaaaagaca ttgaataatt agttttaaat caatttaaga tgaaaaagaa attattaggg
    45181 gttgtattta gtttatttat actgacagga tatatttctt gctcacagcc ttcaaaagaa
    45241 aagattgaac ctaaaattaa atggatggat tttgaaagcg ctgttgctgc atcacaaaaa
    45301 aatccaaaaa aactttttat tgacgtttat accgactggt gtgggtggtg caaaaaaatg
    45361 gatgcatcaa cttttatgga cgactctgtt gcagcctata tcaatgctaa tttttatccg
    45421 gtaaagttta atgcagaaag taaagacacc atccgtttct ttgatcaggt atttgttttt
    45481 gttccggatt ataaaagcca tgaactggcc atatctttac tgaaccggca aatgggctat
    45541 ccgtcttatg tttttctgga cgagaaattt gcttccatca aagtacagca aggatatatg
    45601 tctgttgaaa ctttaatgcc tcttttgcat tactttgcca aagatgccta caaaacaaaa
    45661 acgtttgagg agtttacgaa gtagtaatct tgaaatattt ttagcgtctt gccggcaagt
    45721 cactttaatt taaacaaatt tgttactcca ccaccaccaa ccgtgctgtg cccatgtttg
    45781 acaggcccct aatcactaca gtatagacac ctgctgccag cgcttgagtg cttagtgtgc
    45841 ctttaccttt ttgaaaccta cccgaggcta cctccaagcc catcatattg atgacagaat
    45901 aactgccatc atgttttcag attgctactc aacagaaata ttgtccttca cacactctgt
    45961 gaggcgttta aggggctcct ttttgaagga agcaaatttt ttgataatga ttgttgggct
    46021 tacagtaaac acttcatttg tccgaacttc actttcatat ttcagttctc ggtcaatgtc
    46081 tgttggtgca atagcaaaag aaaacttatc tcctctgatt acagaagtga tttttgcaac
    46141 gatgtaattc tgtttattaa tggaatcctt tgaaataata accgctggtc gttgcttggt
    46201 aattgaaagg tcggtataag gatacggaag taatatgatg tcgccttggt tatattccat
    46261 agctattaat agttgttcca atgttcatct tcgttgctgc cccaatcagt tgctaatact
    46321 ggcttggata agttataaga gctatgaaca tcggctatgt cttggagatt aaaatataaa
    46381 aagtcaacgg tttcaaaaaa atccaaagct gcaatcttga agtcgcggaa ctctctgttg
    46441 tcaattggtt caatgacagt tctgagtttt gataacactg gaattatggg actaattgag
    46501 tttaactcct tagatgcttc gtttggcgac aagtctttga gataatacaa agagccacgc
    46561 aatttgattg tagaatcttt aatcttcgga atggcaaact tctcatattt ctcccaaata
    46621 gtttcagcga tattgaaact tgaaagaacc ccaagagatt ctaaaatgtt tgttgtaaat
    46681 gttaggctat aaaccatgtc gcaaagatac aatttaatag cggtctgtca atgatttaat
    46741 aggaaaagtc tgttcgttaa acaataagtg ttcgggttat gcagtgttta cagccattgc
    46801 cgtgggtagg cttttgaaac caaaatctat ctgccagcac caacgtttat aaattgctta
    46861 aatgtgtcta tttgcataac ccgcccatgc gtgccagcca ttgaaaactt tgtgcctgcg
    46921 ttacattgaa taaacttacg taggaagaaa taagaagtaa aagtaagatt acttttttca
    46981 tgataaattg ttttttttaa ttactttttt actttgttac tccaccacca ctaaccgtgc
    47041 tgtgcccgtg tttgactggc cttgtatcac tacggtatag acacctgctg ccagcgcttg
    47101 agtgcttatg gtgcctttac ctttttgaaa cgtacctatg gctacctcca agcccatcat
    47161 attgatgaca gaataactgc cattggttat tgccgtagca aaggttactg cctcaccttt
    47221 ggcaacaggg tttggccaca cactgatgcc tgctaccgtt gctgcagtta aatccggact
    47281 catgcccgtt gtgcagttgt agccatactt ggcaataaaa ccatcggaat ggcctcctgc
    47341 actttgcaca ctgtgtgttc ctgcagttaa tatgttacta aatgaacctg caatgtaaat
    47401 attgtttaaa ttatcttttg ttgctgcgtt tatttcggtg gcacctgcgt tggttaatct
    47461 cacagcattt aaagcatttc cgttactatc aaaacgggct ataaatgcat catcgttacc
    47521 agctccaacc cacgaatcct gcaacgtatc atttcccact atagcaagac ccctatattc
    47581 ttttgctatg gttaaattgt cgggcatggc gctatcaatt ttgcctgagt aaccaacata
    47641 cacttgtgtt gcccatacag gttgtcccaa gggcgacagt ttaattagga aagggcttgc
    47701 accagaaggg gcaataggat ctgtgaatac aaaattgcca cacttaaggc ctatgtaagc
    47761 aagtccggtt acaaaaatat tatcgtttgc gtctattcta caatcaaatg caagtgagcc
    47821 gttaagacct atggttgtat cggtaaacat atacgaccaa tcaaaaatac cggaatttga
    47881 aaatttaaac acaagggctt gatagctgcc cggtttggga ttataaatag tttggttacc
    47941 taccaaaacc gaatcttcgg taaaataact gttgagtata taattgtcct gcgaatccat
    48001 agtaacacaa tatgcgccca cagaataatt agtactaaag gtaaatattg attgcataat
    48061 gcctgatgta tcaaaccttg aaatataggg tgctgtattg ggctcagtgg tgttccagcc
    48121 ggggaataat aatccagggc ttgaaccaaa tgaaactacc ggttgcccat tgctatctaa
    48181 taataccttg aaacctcttg acccaccgct aatggcagcc gtacctgctg ctttaatcca
    48241 taagtaattg ccacttgtat caaatttagc aagaaacata tctatatgaa catcagtaaa
    48301 ggtagtatca aaaaaagtgc agggccggtt aggtgtagat gaaattcttc ctgttaagta
    48361 aatatgccct atattatcta atactagatc gccagcatca tcttcgtcta aacctccagc
    48421 agttttaacc cacacaaggt taccatgaca atcatacttg cataaaaaca tatcatagcc
    48481 tccataagta gtaacaggca caccgccaaa attggctatg gagcgtatgc gtccgcaggc
    48541 ataaacgttg ccatcggcat ctacctcaat atcacgtacc cattcatcgg ggtcaaagtt
    48601 gaggtcagag tctggcccgc ccatgcgtgt cagccattga aaactttgtg cctgcgatac
    48661 attcagcagc cctgtagcaa aaaaatatat actgagtaat aaaattgctt ttttcatgat
    48721 aaattgtttt ttaaaatgac cattatattt tttttacact attggttggt gtactccttt
    48781 tagccgctgc cctaatgcag cggcataagg agttaagcta ctatttaata acactgaact
    48841 tttgtgtcag tacggttttg ccgtcagtgc ttattgtaag gtaatacatt ccttccggaa
    48901 gcatggacgt attgtacctg acaaagccgg tgtttgccat tatgtgcctg atgttgatga
    48961 ctgcaccacg ggcattggtc aatgtcaata cactgtttag gtctttgcct gatgcagcag
    49021 gcaaaacaaa ctgcaccaag gcttggctac ttgccggatt gggcatgatg gtaagctgcg
    49081 gctcatcagc atgttgctcc ttgagtgctg ctgtcaggcg cgtgttgccg gtgccaccac
    49141 cattgcaatc aggtaaatta aagcatttat aaacaaagca atagtcaccg gtcatcacat
    49201 catagaaatt aacatacagg cacatctgtc cattggtggg tggcaaatcg gtaaagattc
    49261 cttgcaaggc agtgatgccg cctccggtgg tgcttgcagg aaagcctgtg agggtgccgc
    49321 tgctaaggct gtacatggca ctccagttgc ccggtggcag actcacatta aggttaacgg
    49381 cataggtctg attgccattg gcatcaaggt tgatgcagta gatttcgtcc atttccatct
    49441 cccatccgca ttttggggta gggcagtccg gaggcttcac acaaaaggtg taggtgcagc
    49501 tcaatgatgg gtcagggtcg gtgtaggtaa gggtaaagca aggtaatata tcgtctattt
    49561 gataaaagcc caaaataccc tcaatcatca catcgttgcc atttacctgg taggtccatg
    49621 gggtgatgtt ggtattgcct cccggtgggt tgggtgccag cgtaaaagtg cccgtaggta
    49681 tcaggttgtt gaaatgcacg gtaaactgaa agtggtaaaa cgactgtccg tttaagtctg
    49741 tgcctaagca ttggtaccag tcttcgcgca cctcggttat cttacatggg caggtacgaa
    49801 cagtaacata aatagggtcg gtagttattt tgcaccccat gtcgctcacc aattccaact
    49861 gcacaatgta ggagcccggt gctaaggtgc caccaccggc cagcgcattg ttaatgtcca
    49921 gatcgcccac agggcttgca taaccggaat aaggagcatt caggtattgc gagttaatta
    49981 tccatgtcca gcgggcgtag gatatgccat agctaccggg tatgacaatg tggttttggt
    50041 cattgtcgca gcgatccaga catccgctga cgagccagct tatatcaggg ttggcattaa
    50101 ccctaacaat ggcgctttgg ctggtgcagc cagatgcagg gtcggtaaat attgcattgt
    50161 aattgccggt gttaggcacc gttatggggt tggtattgcc gccctgactc cagttcagta
    50221 ctaaggttgc agcccagtta gttgccgtaa gggttacggc attgttttcg cagccgggtg
    50281 tgggctgcac cgctactaaa ggcacagtcg gagggtcaag tacgttaaat gttttagaca
    50341 ccaaggtgct gcagccgtca gccgtagtca gcatcagggt aacggtgtag gtgcctgtgg
    50401 ccggaggtgc tatgtgaatg gctgaggcct gaaggttggt gctgggtggg gcaagcatta
    50461 atccaccggg cagtggctgc cattcatagc tgccattgcc gctgtagccg tcaaaaaaca
    50521 ccgcttcgcc ctggcaatag tcactcttgc ctctgactat caggttgggc acattatata
    50581 ccgacacatc ggcaaatctg ggtgatacat atttgcagcc gctggcagca tcaaaaacgg
    50641 taatgtaata ctgccctgta gaatgcaccg gtagcgtagg tgtaacagca ccggtactcc
    50701 ataaataatc agtaggaata atagaattac ctccaccctg caatacatca ggataatatc
    50761 ctaaagtttt agaagcaccc ggacaaaaac tgtttaatgg tggtattgga aagccattta
    50821 atctattctc ataaacagaa acataatcat catcggtata ataacaaccg tagttatcat
    50881 agacttttag ttctacatga tatgtaggaa ttgaaaaagc atcatacacc ctttgcgcat
    50941 caatgtgttg cacgctagtc gggaaaagcg gagcatagga ggcaccgtca ccaaaatccc
    51001 acagggcatg atgtacctga gtaacagagc cggtgaaatt aaaattaacc actgtttgtt
    51061 tttcgcaaaa aggcggattg ggggcaatgg taaaggcagc accgggaggt gtgggtatca
    51121 acaactggtt gagggtgtta ttttgggtgc aggtgtagcc cgaggaagta gttacggtaa
    51181 cactaacggt atgattgccg gtggggctta ccggaatagt attgctgatg gggccgttgc
    51241 ctatgttgcc accatcaaac aaccattggt agctgtttat ggggtttgta ctgaagacac
    51301 tggcattgaa agta
//
LOCUS       DAOQNP010000009        50666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_192_length_50666_cov_10.242477, whole genome shotgun sequence.
ACCESSION   DAOQNP010000009 DAOQNP010000000
VERSION     DAOQNP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 50666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50666
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192_length_50666_cov_10.242477"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKY09_02355"
     CDS             complement(<1..285)
                     /locus_tag="PKY09_02355"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNB11725.1"
                     /translation="MKFGVVVFPGSNCDEDMVFVLRNILKQPVEKLWHKEHSLRECDF
                     IVLPGGFSYGDYLRSGAIARFSPIMQEVMEFAAKGGYVLGVCNGFQILCES"
     gene            complement(346..1080)
                     /locus_tag="PKY09_02360"
     CDS             complement(346..1080)
                     /locus_tag="PKY09_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HNB11726.1"
                     /translation="MFSFFKRKENTAANFLEVKTDMHSHLIPGIDDGAKTIEDSLALI
                     KELHALGYTKLITTPHIMSDFYRNTPETILAGLEEVRNAIKAENIPVTIEAAAEYYLD
                     DGFIHKLEEEKLLTIGNNNHLLFEISYVNAPDNLLQVIFRMQVLGYKPIMAHPERYPF
                     WGNNFDFYGSLREQGVLLQINVNSLGGYYGPDAKRIAEKLIEKEWVDLIGTDTHATKH
                     IQALHKTVREKSFKKLLEFNLLNKHL"
     assembly_gap    1142..1241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1538..1744
                     /locus_tag="PKY09_02365"
     CDS             1538..1744
                     /locus_tag="PKY09_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11727.1"
                     /translation="MKPMFEYTKEILTKVSFDKKLFKKELVKAMRWLKSDERKMLMVW
                     CLATFGHKYSDVLTEVFRKVTHQG"
     gene            complement(1751..2677)
                     /gene="queG"
                     /locus_tag="PKY09_02370"
     CDS             complement(1751..2677)
                     /gene="queG"
                     /locus_tag="PKY09_02370"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNB11728.1"
                     /translation="MNRSQTTAWVKAEALKLGFSHCGISKAGFLEEEAPRLEQWLKAN
                     MHGTMSYMERNFDKRLDPTKLVEGSKSVISLLFNYYPEKKQNPEAPQISKYAYGTDYH
                     FVIKEKLFQLLEHMRQHIGAIDGRVFVDSAPVLDRAWARKSGLGWIGKNNNLINPQSG
                     SWYFIAELILDIELDADAPIKDYCGTCTKCIDACPTDAIVMPHVVDGSKCISYLTIEL
                     KNEIPVSFKNQFKNWMFGCDICQDVCPWNRFAKPHQHEDLKANHEFLEMNQNDWEELT
                     EEIFKKKFKDSALNRTGFKGLMRNLDFLNQKK"
     gene            complement(2684..3718)
                     /gene="ruvB"
                     /locus_tag="PKY09_02375"
     CDS             complement(2684..3718)
                     /gene="ruvB"
                     /locus_tag="PKY09_02375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNB11729.1"
                     /translation="MNKNLDPASENLSTAEKEIERALRPLSFNDFAGQRKVTENLTVF
                     VKAAVQRGEALDHVLLHGPPGLGKTTLANIIANELNVKIKVTSGPVLDKPGDLAGLLT
                     NLQANDVLFIDEIHRLSPIVEEYLYSAMEDYKIDIMIESGPHARSVQLKINPFTLIGA
                     TTRSGLLTSPLRARFGINSRLEYYDARLLEDIVMRSASILKTAIDEEAAFEIARRSRG
                     TPRIANALLRRIRDFAQIKGNGTINIEIARYGLQALNVDIDGLDEMDNRILNAIIEKF
                     KGGPVGLNTIATAVGEEAGTIEEVYEPFLIMEGFLMRTPRGREATEKAFKHLGKNSLK
                     QRGTLFDSES"
     gene            3884..5974
                     /locus_tag="PKY09_02380"
     CDS             3884..5974
                     /locus_tag="PKY09_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB11730.1"
                     /translation="MKQRFIIFRQRIFIRKRLDNIVVMKSIIAILIFILLCNTGQATH
                     IMGGEFTYTLIGSVPQQQKKVYKISLNYYRNCDTSAAQFQTFYDLYICYDSSNTNYKR
                     LYTQISLDKINTQPVTLPVLDTTCYYNNNWCVEKAYYEATVILSEDTTWHLFLNDGAR
                     NVSINNLIQPDSTGIFFYSMIPAGISNSTPQFSSLPTPFICNLDTVQLSNLAFDSDGD
                     SLAYKLVRPYGNLGVNNLMTYQDINLNLYFLFPLPTVIYSPGYSYTSPFGAGGFCSLD
                     SQTGLLTISSPNQGLYVLTIEVEEYRNGALLSGTRHDMQVIVAACSYNTAPQLTSPVV
                     LNYQIDEGDSLCFPLTFADAENNQINISATGNLFDAALNNPPALFVVNTAPNNNTAQF
                     CWKTICGQAAPFPYMFTVAITDNGCPSKTNTYVFTILVKSISHNQLPSITLSMFPDTA
                     ICTGTAIRFNATTQFAGNNPTYQWYINGQAISNNYPDYITNQLNNGDSVSVQLISNSI
                     CVIDSGAISNTQVATVLQPTVASFITNTVSVNSINALQQFTNLSVGNTYNYWNFGDST
                     QSVDISPQHVYVGSGIYLVQLITVSSDGCRDTTEVPVDIKTTMSFFIPSSFTPDANGL
                     NDWFAPIGAEMQDYTLQIFNRWGQLLFSETGKPQWNGRFKNQLLPLGTYIYSIRMNNP
                     TDPIIKTGTVMLLR"
     gene            6165..6917
                     /locus_tag="PKY09_02385"
     CDS             6165..6917
                     /locus_tag="PKY09_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11731.1"
                     /translation="MLKRFVCIILSLLTFTGLKAQDNKTEKKDSIEHRILLIPYDPRF
                     YLSDADKDIAEETRKDPETIRNYFHNRSEWFTWRELKRHYPTISLLQNDTVPAYIQSA
                     GDLFSQTAFDYATPITRSPESLMNATSDKTLTDSRTATNYLNTDIHNRYMKADIQNKK
                     VLADLSERFNTDLFVFLTQFEIKTNYKNCLDIANKVYEREIRLHFTIYDKTGKLINGN
                     YAIAVIPSNVNQMDEIILKCFPLLAKGIAASL"
     gene            7000..9246
                     /locus_tag="PKY09_02390"
     CDS             7000..9246
                     /locus_tag="PKY09_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB11732.1"
                     /translation="MRKSLTLSTIFVILLGAQTLASNFWTVKSETQEMLSGKRQIVPD
                     KYMVYTLDVQGLKNQLLNAPLEFTMAAKQSTVVITLPMPDGSWNSFSMVESPIMQPEL
                     AAKYPFIKTYSGSGINNQPSWVRIDFTQWGFHALIRTAENDIYIDPYSMQTTSDYLVY
                     FRKDFTQSQSSMVCEFDDLQQGQGTNKALTNSLPAMNRSIGTNLRTYRLALACTGEYA
                     AYYGGTKAGALSGMVTSVNRVTGVYESEVDVRLNLIANTDTLIFLDANTDPYTNNNGG
                     SMLGQNQSVVNFFIGTPNYDIGHVFSTGGGGIAYLGCICKTADKAKGVTGSSAPINDP
                     FNIDYVAHEMGHQFGGNHTFNSVTGSCQGNREASAAFEPGSGVTIMGYAGICGSDDLA
                     AHSIAYFHTKSFDEIVDYSTLSTGNSCPVNTITFNNAPTVSGGGIFNVPVGTAFKLDG
                     YGSDPDGDSITFSWEQFDLGPAGAWSLPTGNAPMYRSYAPSTSALRLFPKLSVLLNQV
                     NAKGELITSYARSLHFRLTARDNKPNGGGVTYNDTITTVNIVQTAAPFAITYPNVTGI
                     SWPALSTQTVTWEVGGTDAAPFNEQFVNIYLSINNGTTFPITIATNVANNGSYTFTVP
                     NNQSNICRMWVEGASTGSIFFDINNKTFTITAPVGINEVNSLSQLTTYPNPADNELLV
                     YIGNSNHGELSLRLFSVNGQLVLSQTIAAKDAAAQVALNVAQLPAGLYFLKAETENGT
                     AERKIIIK"
     gene            9366..>11065
                     /locus_tag="PKY09_02395"
     CDS             9366..>11065
                     /locus_tag="PKY09_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11733.1"
                     /translation="MKPFFKAVFTFLFTITILHSADAQFYQGSQLSFGKNRVQYNDFY
                     WTFYRFKNYDVYNYVGGKELATYVAEQAQNEMDAMERTLDYRSSSRFQIVVFNKLSDL
                     KQTNIGLELDDQTYNIGGMTKVIGNKVLIYFDGNHQHLQEQIRAGMARALLEQLFYGG
                     NIRERLQASILLNLPQWYTQGLVTYLSKGWTSEDDNKLRDYIMTEKKPVLNKLIERDE
                     IFAGQSLWHYIITTYGEASLSNLVYMTRINRSLESGFVFVLGVRLKQLSADWLSYHTK
                     KYQEFEKNKLPIAAIPLKIKEKNKLIYQVKSDPFGNRVSYVTNKRGRYKVKIADAKTG
                     KRTCADRGGYVSLTTENDQSFPVTAWHPSGKILAIVKEQKGNIILNLFNTETKKSDFS
                     EIMSFSKILSMNYSPDGSKLLMSAVQKGQSDIYVFNVRGRNFDQITNDVYDDLYPQYD
                     ISGSKIIFSSNRSNDTLGVDVKQNPPQNNFDLFMADLYNREGNLLRITHSPEDETFPV
                     MSDSLRISYLSDKNGVINRFVSEMDSTISFIDTTEHYRYFFRHYALTNSNRNILEQDF
                     S"
     assembly_gap    11066..11165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11166..12521
                     /locus_tag="PKY09_02400"
     CDS             <11166..12521
                     /locus_tag="PKY09_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11734.1"
                     /translation="FKSKKKKEEPIENKSVATDTTKIVQEQVKLQPDSNGVELTPDSA
                     IFRMPKQRNYDISFSSDYILTQLDNSLFNTTYQPYTGGAVYFDPGMNALFKIGMSDLM
                     NDYKISGAFRIGFDFNSNEYLLMIESLKKRIDKQILFSRQSKLFSVSDLDVLRTHTYN
                     LKYSGKYPFSEISAVKGTIDARMDKTIYVATEKAQLEKSDIINYWASIKGEYIFDNTI
                     NKALNLYNGTRFKIFAEAFRKINKPEKMVYIIGADFRHYVKVHRQIIWANRLSASTSL
                     GKQKLIYYLGSQDNVIVPGNNFNNEISVDKNEEYVFQTLASPMRGFKQNIRNGSSFAL
                     FNSELRIPVFQYLLNRPIRSDLIRNFQAVVFFDAGTAWSGESPFSDKNGLNEEVIGGG
                     GSNPVTVTVIKQIQPIVGAYGFGFRSRIFGYFIRADWGFGIDDRQVQKPLFNLALGLD
                     F"
     gene            complement(12522..13784)
                     /gene="rodA"
                     /locus_tag="PKY09_02405"
     CDS             complement(12522..13784)
                     /gene="rodA"
                     /locus_tag="PKY09_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNB11735.1"
                     /translation="MRKQKSIFQNIDWLLVLLYLVMVIAGWLNIFAAVYNEEHSVITD
                     FSQNYGKQMMWILTSLVIAFFILIIDGKFYDAFAGVIYAISIISMIAVLFFGHTVAGS
                     KSWFRFGDFGIQPAEFAKFATAMMIAKFLSPLGLKKNTWRTRISAVAIFFFPLLLILL
                     ENETGVALVFLSFCIVLYREEIIPGWLLMIGISAAVLFVVSLLVPKMILVTSLLILAT
                     IIVLLFRKWKTFFVVAGLVAICIGVVVSVDHVFNHVLEPHQKKRIQVFLGMEDDLKGA
                     GYNVNQSKIAIGSGGLTGKGFLKGTQTKYNFVPEQSTDFIFCTVGEEWGFVGTTTVVL
                     LFLALLYRIIFIAERQKSAFARIYGYGVAGVIFFHFMVNVGMTIGLMPVIGIPLPFFS
                     YGGSSLWGFTILLFILIKLDSYRTFVLR"
     gene            complement(13788..15629)
                     /gene="mrdA"
                     /locus_tag="PKY09_02410"
     CDS             complement(13788..15629)
                     /gene="mrdA"
                     /locus_tag="PKY09_02410"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNB11736.1"
                     /translation="MKTSHSTQRLVLLITISLVAFTYIGRLFYLQVVEDKYKLSADEN
                     VLRRIIDYPSRGLVYDRNGKLLVFNQTVYDLMITPREVKEMDTAEFCSIIKISIEDFR
                     KKFQKAKNYSPYRSSIFEKQISAETYGTLQEKLFKYKGFSVQPRTIRKYPYNTAAHLL
                     GYLGEVDENVTAKNNYYQMGDYIGISGIEQSYEKELRGRRGTRYMMVDNFNREKGRFK
                     EGKYDTLAVSGENLKLSLDAELQEYAELLFQNKRGSAVAIDPSTGEILAMVNSPTYNP
                     NLMVGRERSRNYGRLLKDEDKPLFNRAMMAYYPPGSTFKIINSLIGLQEQVITPETYF
                     SCAGGYRVGNIFVHCHPHSSPLDLRTAVAVSCNSYYCQTFRGVIDNRKYNRTEDAFDA
                     WRNYVLSFGIGKKIDVDMPHELRGNVPTVDYYNRFFGKGRWKSSTIISLAIGQGELGI
                     TPLQMANAACIVANKGYYYVPHIVKEIGYKHYLPELFKKKNYTMVDPKYFNAIQDGME
                     WVFERGTARGSRVDSLVMCGKTGTAQNPHGKNHSVFFCFAPRENPKIAVAVLVENAGQ
                     GAHFAAPIASLMVQKYLLRTIKRPDLEKRMLDAVIVPMANDSTVLDD"
     gene            complement(15641..16159)
                     /gene="mreD"
                     /locus_tag="PKY09_02415"
     CDS             complement(15641..16159)
                     /gene="mreD"
                     /locus_tag="PKY09_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNB11737.1"
                     /translation="MLPEVIKSVLRFFLLILLQVVLLNKIRFAGYINPFVYPLFLLLL
                     PVRIPKILLLILAFITGISVDIFSDTLGMHAAASVFLGFMRPYIIKLLAPRDGYDSDA
                     VISLKQMGILWMVYYLFISIFLHHLFLFYTEVFRFNDFGATFLRVCLSTIASVVIIFI
                     ILLLFDKTKVQR"
     gene            complement(16140..16940)
                     /gene="mreC"
                     /locus_tag="PKY09_02420"
     CDS             complement(16140..16940)
                     /gene="mreC"
                     /locus_tag="PKY09_02420"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNB11738.1"
                     /translation="MFILLEAVCFFLIAQNNSYQNAATLNSTNKVVASVYSTISSVTD
                     YLNLVENNKRLAEENAHLRTLLPASYFDTTVVRNHKVDTIHNLEYSYIEAKIINNSTN
                     RRNNYMTLNKGSDAGIAPEMGVITGHGIIGIIKNVSAHYSTVTSFLHTQSKVSVRFKN
                     NNYNGSMVWDSDNSPTEATVIDIPRHVPFKVGDTLVTTTFSPYYPEGIMVGTVKSFDI
                     DAGSNSYRIILKLSTSFNDVSYVYVVNNLLKNERKQLEEKQKDAARGN"
     gene            17174..17401
                     /locus_tag="PKY09_02425"
     CDS             17174..17401
                     /locus_tag="PKY09_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNB11739.1"
                     /translation="MEKKLSELKPGEAGVIQNFSDEEIKLKLMEMGCIPGETVLMERV
                     APLGDPMAIKVAGYQLSLRKNEAETVWVSTL"
     gene            17419..19542
                     /gene="feoB"
                     /locus_tag="PKY09_02430"
     CDS             17419..19542
                     /gene="feoB"
                     /locus_tag="PKY09_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNB11740.1"
                     /translation="MSEVVLKVALVGNPNSGKSTLFNVLTGLNQKVANFPGVTVEKHV
                     GYFSLRNAFEGVDYKVELTDLPGIYSLYPKTLDEQIPYKVLTDPQDPSFPDRIIVIAD
                     ASNLKRSLFLCSQIIDLKIPVVLVLNMMDLAVHKGLLPDVKKLEQALGIKVIPAIVRQ
                     KTGIEEIKKAVLHTIPLPENDIMPATSLAPELIQEIKHSFPVKSDYAAYLLAGNYDKV
                     DISFVGNDGKQKLDTLLQQHQFGQKAMQATETLERYKTISVLLKSCVSEPASVRKSLT
                     RTVDNLVTHRIWGYGIFLAILFVIFQFIFNVAQFPMDFIDGTFADFSQKVSELLPKGV
                     LNDLIVNGILAGLGGIIIFVPQIALLFLFITILEDTGYMARVSFIMDKLLRPFGLNGR
                     SVIPLMSGVACAVPAILATRTIQSWKERIVTIMVTPLMSCSARLPVYTLLISLVIPRY
                     YVFGFINSQGLVMMLMYLIGFVAAIFSAWLFKMFVKAKQRDFFIMELPVYRLPRWKNI
                     FFVIFDKVKIFLFDAGKIIIAISIVLWFLSSRGPGKEYAEIEKLYEAKAELPETTQDE
                     LNQLQAQKLEHSYAGWTGKIIEPAIRPLGFDWKLGIALVTSFAAREVFVGTMSTIYSV
                     GSNSENLTIREKMMTEKRPDGTPLYTLAVGISLMLFYAFAMQCMSTLAVVKRETGTWK
                     WPIIQFVYMSALAWVSSFAAFQLLS"
     gene            19570..20199
                     /locus_tag="PKY09_02435"
     CDS             19570..20199
                     /locus_tag="PKY09_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sprT domain-containing protein"
                     /protein_id="HNB11741.1"
                     /translation="MAKVTLEFIEEALKKYVPHKAIRYCIQWIEEHQINLKITRSRNS
                     KYGDYRPPQRGFGHRISINHDLNPYAFLITFIHEVAHLNQWKRKKQIIIPHGKDWKEE
                     YRKLMIPILRENIFPPDVVAALNNYMTNPSATSCSDHDLLRALRNYDREEDKWITLEE
                     LNFGVKFKIRTGRTFIKKEKLRKNYSCIDCRSKSIYFINPITEVMPVDH"
     gene            complement(20193..21605)
                     /locus_tag="PKY09_02440"
     CDS             complement(20193..21605)
                     /locus_tag="PKY09_02440"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB11742.1"
                     /translation="MKPAAVFNHLLKNFPFEPTNAQKHAFAELSEFVTENTAYKCFLL
                     KGYAGTGKTTVINAMIKTLYHYDFRVIMLAPTGRAAKVINHYTGDDASTIHRYLYEIK
                     SDKNGDIKTKVKPNKYKKQIFIVDESSMISNEDDKTLFKGASLLSDLIDHAFSQADNA
                     IVFIGDTAQLPPVGLQCSPALDQNFLQNNCQLQVRQYELKEVVRQQLHSGILANAHCT
                     RLLLEREERFPRLKTSGFDDVKQVTSSELSEVLSGYFPSGDDSTVTVLCRSNKQANRY
                     NEFIRKTFYGFEEVVEKNERLMVVKNNYYWHESDKDNAFIANGDEIKLIRLKRQEEYF
                     GFRFANATIELSDEKSTKLEVKLLLDTLNSEAPALTYDEYKKLFDEVRNHYRKADGKV
                     NMRKLREDPYLNALQVKYAYAITGHKAQGGQWETVFIDPGFMTEEMYGVDYMRWLYTA
                     ITRARKKVYLINVPDRFFVQ"
     gene            21817..22830
                     /locus_tag="PKY09_02445"
     CDS             21817..22830
                     /locus_tag="PKY09_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012800314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB11743.1"
                     /translation="MLSIDIHTHILPEKLPDWKSKFGYGGFITLDHHKSCCARMLRDD
                     GTFFREIESNCWDAKTRMHECDVHGVHVQVLSTVPVMFSYWTKPADGLAVSEFLNDHI
                     VGIIQQYPKRFVGLGTVPLQAPELAIKEMERCKKIGLKGIQIGSHVNDWNLNDVNLFS
                     FFQACAENNMSLFVHPWEMMGQKEMQRYWLPWLVGMPAETSRAICSMIFGGVFERLPN
                     LRVAFAHGGGSFPSTFGRIKHGFDCRPDLVAVDNTASPENYIGKFYFDSLVHDENMLH
                     YLINFTGANRIALGSDYPFPLGENIPGTLIKNAKLDLETKERLLGGTALEWLDLSKEL
                     FLK"
     gene            22956..23570
                     /locus_tag="PKY09_02450"
     CDS             22956..23570
                     /locus_tag="PKY09_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNB11744.1"
                     /translation="MDIRERILESGLELFLAHGVRRITMDDIARHLSVSKKTIYACFD
                     DKDEIVSECCQGYLKNSRCEFEHVETESKDAIHEIFLTMEKLQSMFTTINPALFEDLQ
                     KFYPRVWSMYVQFKDKHVAEVIERNLLKGIEQKLYRPDINIKILVKLRIEEITLGMNT
                     TVFPLKSFITKDVQIALLDHYLHGIVTVKGHKLINKYKNITDEE"
     gene            23582..24931
                     /locus_tag="PKY09_02455"
     CDS             23582..24931
                     /locus_tag="PKY09_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB11745.1"
                     /translation="MLNQIKFLTGLLLLISINIQAQQSDKKSYSFSLKQAIDFAFQNQ
                     TDIQNAVLDQKSAQSRANELLGIGLPQISASADITHFIDIPTTFVPAEFFGGEKGSYA
                     PVQFGQPYSSTAGLSASQLLFDGSYLVGVKASRMYADLMRKQVIQTKTEKAALISKAY
                     YNVQVNSERLQMLDANVKRLKKQLDEMQVMYDNGFIEKIDLDRISLAYNNIETEKANA
                     DRLIVLAQNLLKFQMGLNVSDELVLTDKLEDVKVVADNNLNQNIDISNRIEMDVLNTT
                     KELLRADLRLKKSSYLPSLAAFGSYSVSASRNDFDIFENKPWFRTSIIGVKLNVPIFD
                     GFQKAAKVNQARFALNKIENQIKTATQGFQLEAQTAKTTLTNALGSLANQEKNRTLAA
                     DVARITKIKYQQGVGSSLEVMDAETALKVAETNYYAAMVEAFIAKVDFEKATGTLIK"
     gene            24959..26077
                     /locus_tag="PKY09_02460"
     CDS             24959..26077
                     /locus_tag="PKY09_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB11746.1"
                     /translation="MKKVIIAITAIGFLTACGGGNSSKKVEELEKLRKQQSELNEKIK
                     SLEAEIALTDSTRKDKIKFVAATDMSLKPFTHYIEVQANVEGDEDVMLSAKTPGTVTS
                     VNVKAGDHVTKGQVLATLDDQIMQKAIAEVQNELNLLTTLYNRQKNLWDQQIGSEVQY
                     LEAKTRKEGAEKKLASIQEQWDMTRIKAPFSGTVDNVMIKTGQLVSPQVPAIRVVNLE
                     MLKVKGELPETYIGRVNKNDHVLVYFPDLGKEITSKVYYSGNAISNLNRTFNVEVRFG
                     NKENFLRPNMLAVLKIADYNKDKAMSLPVNVVQKGLDGEYVFVVENSGGKTVAKRKTV
                     KSGMTYNGNVEIVDGLNEGDKVITTGYHDLVEGDIIKL"
     gene            26092..29484
                     /locus_tag="PKY09_02465"
     CDS             26092..29484
                     /locus_tag="PKY09_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB11747.1"
                     /translation="MKDIFKEFKPSSWAIDNRTAIYIITIIITLTGIMAYINIPKESF
                     PDISLPNIYVSVVYPGTSPKDMENLVVRHIEKECKGISGVKKIKSNALQDYCSIVIEF
                     NSDVKIEDAKRKVKDAVDRARNDLPQDLPNEPDIIEVNFSDLPIMAVNLSGDYDLAKL
                     KRYADEAKDKIESLKEVKEVKLIGELEREIQINVDMLKLQSSGLTMSDIERAVKYENM
                     TVPGGSVLMADGTRRSLSVSGEFTNVDQIKNLVVNSINGAPIYLKDIAEVVDSYKEQE
                     SYARLNHKNVITLSIIKRSGENLIDASDKIVSVVNELKKEAWPKDLNVVLTGDQSEQT
                     RTQLHDLINTIIIGFILVLVVLMFFMGTSNAFFVAMSVPLSMCVAFLVLPVIGFTINF
                     IVLFSFLLALGIVVDDAIVVIENTHRIFDNGKTDIVRAAKMATGEVFLPVLSGTLTTL
                     APFVPLAFWKGIIGKFMFFLPITLIITLLASLVVAYIINPVFAVSFMKKHEEKNDPYK
                     GLKITSVIFIAIALLFYVTGVYGMGNFTIFIFLLNLLHRFWLHKVIIRFQTKTWPAVQ
                     ELYKRMLTKVLHRPRTMMAGTVGLLFLSFIAIGLRNGGVNFFPIADPNFVYVFTTLPV
                     GTDQAYTDSVTKILESKVYKVLGEKNDVVKSVIASVGVNVSDPSDEDQGYYANKSKIE
                     VAFVEYSKRKGVSTSNYLEKIREAVKNTVPGAEVTVAQEQGGPPVGKPISIEVSGDDL
                     QAITTTSKSLKRYLDSLQIEGVEELKSDLQDSKPQIVFDINREHMNRLGIMTGTVGNE
                     INMAVLGREASKYRDLNDEYKIMLRYKLDQRYDVSLLNNLKILYRDMSMGGLVRNVPL
                     ASFASVKYDNTYGVIKRKNQKRVVTISSNLLTDYQSREAGVVGNVMAAVKQFPKTEGV
                     EIKFAGSQEEQAETMSFLGTALLISLGLIFMILVIQFNSIGKPIIILSEIVFSIIGVL
                     LGFALFKMGFSIVMTGIGVVALAGIVVRNGILLVEFTELLREQGVPTFEAIVEAGRTR
                     MTPVILTATATSLGLIPLAVGLNIDFVTLFTEFNPHIFFGGDSTAFWGPLSWTMIFGL
                     MFATFLTLFLVPVMYLMSENLKAKIYKRPKPIMKKPDDELIA"
     gene            complement(29700..31031)
                     /gene="rseP"
                     /locus_tag="PKY09_02470"
     CDS             complement(29700..31031)
                     /gene="rseP"
                     /locus_tag="PKY09_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNB11748.1"
                     /translation="MSGLIMAAQLILGLSILVTLHELGHFLAARMFGIKVEKFYLFFD
                     AWGIKLFSFKKGDCEYGIGWLPFGGYVKIAGMVDESMDKDQLAQPAQPWEFRSKPAWQ
                     RLIVMVAGVVMNVILGIAIFSMSLLHYKGSYLKNDSVTHGIVAYKLGEEIGLRTGDKV
                     VAIDGKSFDRFDDLLSTRVLFGATLTVIRNGETIPIEVPDDFYKKTGPNARSLFLGAG
                     HLTYVVKEVVVGENAFKAGVENGDIITEVDKQKITGEDFMQRYFADKKGETALLSMIR
                     NGKPMDITVNISSKGTIGVRYNGDVVIPEEYIKTPYTLSSALAFGTSDAFETLVSNAK
                     GFKKLFKGEEKFQESVQGPIGIAKIYGGTWDWPHFWVLTGLLSMILAFMNILPIPALD
                     GGHVLFLLIESVTRKKFSDAFMEKTQVVGMVLLLSLMVFVIGNDIWRAFIK"
     gene            complement(31057..32217)
                     /locus_tag="PKY09_02475"
     CDS             complement(31057..32217)
                     /locus_tag="PKY09_02475"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNB11749.1"
                     /translation="MKKRIAILGSTGSIGRQALEVIAAHADVFEVELLTANGNIELLE
                     KQVRQFTPATAVVTDKEKYKIFKENIADIPVKVFAGIDSVEQAVQSDSIDIVLTAMVG
                     YAGLLPTIAAIKSKKTIALANKETLVVAGKIITALADDNGVRIYPVDSEHSAIFQCLA
                     GEFHNPIEKIYLTASGGPFRGKSKKELEQVTLAQALKHPNWSMGAKITVDSATMMNKG
                     LEMIEAKWLFGLQPHQIHVVVHPQSIIHSVVQFTDGSMKAQMGLPDMKLPILYALSFP
                     HRLKTDFKRFNFLDYPQLTFEKPDTEVFSALLLAEEAMKKDGNMPCILNAANEVAVAA
                     FLKEKISFFQIPGIAEQLMSKITYIEKPSLEDLIATDKETRLQTELLLTKKN"
     gene            complement(32329..34164)
                     /gene="glmS"
                     /locus_tag="PKY09_02480"
     CDS             complement(32329..34164)
                     /gene="glmS"
                     /locus_tag="PKY09_02480"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNB11750.1"
                     /translation="MCGIVAYIGNRQAYPIVIKGLQRLEYRGYDSAGVALLNGELSVY
                     KKAGKVADLAAYVSDKNLVGQVGMGHTRWATHGEPNDRNAHPHYSESKDLAIIHNGII
                     ENYAAIKQELINSGHTFKSDTDTEVLIHLIEEIKHKTHVATEEAVRLALGEVIGAYAI
                     VVLSKNEPNKMIAARKGSPIVVGIGKDEFFIASDATPIVEYTKNVAYLNDGEVATIDN
                     GKLTVKTIDNETKIPFVQELELKLEAIEKGGYDHFMMKEIYEQPRSIWDSMRGRIDAE
                     KGRLRLGGISEFENKLINAKRIIIVACGTSWHAALVAEYLIEDLARIPVEVEYASEFR
                     YRNPIISEDDVVIAISQSGETADTLAAIEMAKERGATIFGVCNVVGSSIPRATHAGSY
                     THAGPEIGVASTKAFTAQVTVLTLMALQIANKRGTITQSRFRALLNELETIPSKVEIA
                     LRSNDKVKEISALFKESRNFLYLGRGYGFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDEEMPVVVIATKNSSYEKVVSNIQEVKARKGKIIAIVTEGDVDVRKMADYTIE
                     IPETDDILVPLVSVIPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(34171..35463)
                     /locus_tag="PKY09_02485"
     CDS             complement(34171..35463)
                     /locus_tag="PKY09_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HNB11751.1"
                     /translation="MQQFKHLWMACVVLLSISCNKPDSVGLNLLPGEDAMNVEFFDTL
                     TLQTSYKQEDSLRTDAHLLYELQATNHQLMIGKYVDPVFGETSAELFTQVKPSAVFSN
                     TNKTYDSLFLNLAYISNYGDTSALQTFEVYELTDAMYDTASYYAFSNLNYSTKVGEIT
                     FKVEDVKDSIVNTDTTKSAPRLRLRLDDVLGQKIFNSDLSDPSKFKETVKGFCIKPAA
                     SSFGSIINFDAKTTVSSMIMYFTDPATTGIDSLPFYMYSSDGSIARFSRFTHDYSAFN
                     FTNPLTDKAYVQTMGGIKTKITIPYLNSLKEMGPIAINKAELIINVDPGTTTNLPAPD
                     ALSLYAIDSVGQIQTLPDLTRSSVLFGGGLANNQYRFNIAQFLQQILYEKRIDYGMYL
                     RIRVPVAFQTPNRVVLTGGNPSSTLRMKLQITYTKLNP"
     gene            complement(35513..36340)
                     /locus_tag="PKY09_02490"
     CDS             complement(35513..36340)
                     /locus_tag="PKY09_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNB11752.1"
                     /translation="MNKKPKVLFVSQEIFPYLESSTIGNIARHLPQGTQEKGKEIRTF
                     MPRFGVVNERRNQLHEVIRLSGMNLIIDESDHPLIIKVASIQAARMQVYFIDNEEYFQ
                     RKHTTHDADNKFFQDNDDRTIFFCRGVLETVKKLGWAPDIIHCHGWMSSLIPLFVKTA
                     YREDPIFRNARVVYSVYDDEFSESFHDSYSKKLMMEGISTNDVKDIKSPNFENITIAA
                     INKSDAIIKGAEKLNTKIDNHIKKSGKLILPYQSPENYVEAYNNFYDEIMEEISIEA"
     gene            36565..37101
                     /locus_tag="PKY09_02495"
     CDS             36565..37101
                     /locus_tag="PKY09_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11753.1"
                     /translation="MKKQIIQSALRLLKLNPLFLFILVCLPIGFIFYFTPAEDFLVFK
                     KLFSSLGWIIYFFWIFAIGHRAIEKVVSQNIAWGGFRYFIPGYVVSAILFIVMLFTTT
                     KEKIQIGLLQVEYIAPLVLTISAAVMFLIVILIASKAIVSAETGSNANLGSFLPTALL
                     LFFFPIGLWFIQPRIQRL"
     gene            37256..37810
                     /locus_tag="PKY09_02500"
     CDS             37256..37810
                     /locus_tag="PKY09_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNB11754.1"
                     /translation="MTALIILIVVLAIVAFYFISVYNTLVTLKNKVQEAWSSIDVMLK
                     KRNDLIPNLIETVKGYAAHERNTLDNVTQARAQSVNAKTVGEQKAAEENMNKAMMNLF
                     AVAEQYPDLKANTNFINLQQELSAIEGDIEKSRRYYNGTARDNNIAIESFPSNIVANM
                     FQFNKVEFFELSNESEREVPKVKF"
     gene            37836..39749
                     /locus_tag="PKY09_02505"
     CDS             37836..39749
                     /locus_tag="PKY09_02505"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNB11755.1"
                     /translation="MRTNRSYLLGIVSLIFILINSNFASGQEERILSYNSDIQLNPEN
                     ELMVTETIKVVAKGIEIRRGIFRVLPENKSKSNPLKDVSYDIVSVKRDGKEEPYHLKN
                     ENGNTVVYIGEKNEYLDPGIYVYELQYKTSNHVGFFNDYDELYWNVTGNDWSFHIDSV
                     SATIHLPASAKILQQSCYTGYAGSTNTNCTSQKLSDTEINWSAVGLNSNEGLTVAVGF
                     PKNIIAPPPPPGFGQKYALLLFAIIVVFLLLIYYFFTWSKYGKDPEAPAVYPIFESPD
                     AISPAILGYVDAEYFKNDFVTASIVNLAVKGYLKINEKENKVLLGLFKDTNYSLTKLK
                     NADSSLPMEEAMLLNKLFSSSNELSLTGTYDPNIKNAVTAYTDSIKSQYSNFINKGNN
                     FKLIVAPSVMIALAIILISIFDAMMIDRLLPLFVIAIFGTVSLFIISFIFGFMGKRFY
                     KTMWLIAILEMGLLLYLSYHTLYIAEDHLENNLTALAIVLLFGAASIMFYTYLIRKPS
                     AEKLKLQSQIKGFGMYLSAAEEEQLKFFNSPKMTPEIFEKFLPFAMVLGADKIWGEKF
                     DNMLKSASIQNTTYNSSWYSGSHLSPALFSSSLNSSFSHSIQSASTPPSSSGSGGGGS
                     SGGGGGGGGGGGW"
     gene            complement(39775..>40365)
                     /locus_tag="PKY09_02510"
     CDS             complement(39775..>40365)
                     /locus_tag="PKY09_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11756.1"
                     /translation="LFGAFSAIIIYTLLFLYEPHKSAYLIPVIPFVILVILIAHQDKL
                     WPMLVTTSLVLSCFFAGINLAEPNRSALPGKYALTRKVGNLRICFDPFSGNVSDDYAK
                     RTQRIKYAVSVIEKTHLLQGRNAVICGYWVNMISILSIGQLNPQIMLLHYADEETLLK
                     LKNENVGIYYLPEQAIYNDLCYKKKFTRHLAQNLMQ"
     assembly_gap    40366..40465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40466..40932)
                     /locus_tag="PKY09_02515"
     CDS             complement(<40466..40932)
                     /locus_tag="PKY09_02515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB11757.1"
                     /translation="MRLILLGLLVFASRWFFLDAGYGAEEDAWGYAVNALAMDSTHSY
                     QYSRLPGHPVPEWAYYFLPVKHSFAFNLCTALLSTVAVLLFYLICKKYKLNATAGALM
                     LAFTPVFYIQSTNAMDYNWSLCFLLAALYFAGRRKIFLAALTLALATGCRITA"
     gene            41046..42032
                     /locus_tag="PKY09_02520"
     CDS             41046..42032
                     /locus_tag="PKY09_02520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HNB11758.1"
                     /translation="MAMLLELTNKGIYCPQANVYIDPHRRVENAIITHAHSDHARPGS
                     SHYLAHKDSEAMLRMRLGEQISLRTVAYGETFTINGVKFSLHPAGHIIGSAQIRVEFK
                     DEVWVAAGDYKLHDDGFCPVFEHVKCNVFITESTFGLPIYKWQPQQQTMQDITAWINT
                     NNQQQTTSVLMGYSLGKMQRLIKQLNHFDFGIYGTSDVVAVNERLREAGCDLPYVKPL
                     KSNFVKENLKGALIFASPSGARSALFKKLENYSVGYCSGWMLHGKSKYFSHVDRGFAL
                     SDHADWDELNTAVKESGAEKIYVTHGSTTAFSKWLCDKGYDAKEIEFMKGVG"
     gene            complement(42080..42481)
                     /locus_tag="PKY09_02525"
     CDS             complement(42080..42481)
                     /locus_tag="PKY09_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11759.1"
                     /translation="MLKDIPQLKVENVAVAIVKEQSNGADVWNAYIINLKNEVLQGVL
                     ITSKGYGIMEGEERKTSTLRHFIENIPPQSWVKIEPITDNVLNLNNEYWVSFYLNNTM
                     YDRKYVFLPGVINEEFFTQIPLLEKRGVMIR"
     gene            complement(42527..43660)
                     /locus_tag="PKY09_02530"
     CDS             complement(42527..43660)
                     /locus_tag="PKY09_02530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB11760.1"
                     /translation="MKKIIKYLLFILLITTIALAATGKLYYYKALVYNFVDIDDLNLF
                     YNREVKNGTGMPWNVSTDYNKKNLSDTLTALLEANKSVAFLVLKNDSICFEKYWENYG
                     QESLSNSFSMAKSIVALLIGKAIDEGKIKSLDQPVADFIPEFKEGEKTKITIRHLMMM
                     SSGLDWNEGYASLTSQVTEAYYGTQLYKQVTSLGVAEAPGKFWEYKSCDTELLAIIVN
                     KATGKSLAEYASEKLWKPLGAEHNAQWSLDQKDGIEKAYCCFYSNARDFARFGKLVLQ
                     HGRWNNEQIIDSAFIALCTTPNNLPDKEDNKPAQHYGYQWWLSNISNHPVYYMRGILG
                     QYVIVIPDMQMIIVRLGHKRSNNPDGKPNDYKFYAEQVIKMYE"
     gene            complement(43657..44394)
                     /gene="lipB"
                     /locus_tag="PKY09_02535"
     CDS             complement(43657..44394)
                     /gene="lipB"
                     /locus_tag="PKY09_02535"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNB11761.1"
                     /translation="MQEIFFADLGSSDYKQTWDFQEQLLQQIMQLKLEGRNQGLGDHA
                     PTPHYLLFCEHPHVFTLGKSGKPEHLLSNEQQLKNANADYYKINRGGDITYHGPGQIV
                     GYPILNLDLFFNDIHRYMRFLEEVIIRTLKEYGIEGSRIEKLTGVWIDAGKPSARKIC
                     AFGVRCSRWITMHGWALNVNTQLEYFNHIIPCGISDKAVTSMAKELGNNLNIDEVKSV
                     LLKHFSDVFNATIKTVEKEKLIAKEKT"
     gene            complement(44460..45713)
                     /locus_tag="PKY09_02540"
     CDS             complement(44460..45713)
                     /locus_tag="PKY09_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNB11762.1"
                     /translation="MLRISFFASLIFIYVLLCTHLVFAQQDDARKVITELSSEKYFGR
                     GYLKHGDKKAAHFIKKRFCKLGLKPVDDKWFQHFTHPVNRFPKNVSLTTDTKTWIPGK
                     EFLIHHQSPSANGTWPVAWADSAEIISSLNNDFDSCYVITRQVFSKLQKEITSLLFKR
                     KKGVLVILEPHKLTWSVGTSVFNIPVIEVYDSLFSRQTKSITLHADAEFNAQYNSRNV
                     IGVITGNKIPDSLIVITAHYDHLGGMGNKTFFPGANDNASGVAMLLSLAEYFTKQENR
                     QPYSILFIAFAGEEAGLQGSKYYTEHPLLPLSKIRFLINLDLLGTGDEGIMVVNATEF
                     ERDFSILQSINDTAKYVSNVGKRGKAKNSDHYYFSEKGVPAFFIYTLGGVTWYHDVMD
                     KSETLPLTKYNNVFQLLKSFIIALQ"
     gene            complement(45713..46480)
                     /locus_tag="PKY09_02545"
     CDS             complement(45713..46480)
                     /locus_tag="PKY09_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11763.1"
                     /translation="MTKQSITSEIQVAIEQIYRQWGVFSLDNRIPENDELDMLKSKLM
                     KLHDKINHLQLVESVSSYEQPNQEVVTETVRQPIVQTVIEKSEPEQRTVVETPKTELK
                     VEALIIEEVKAKPVEEVKSEKNAEPVIPPVQTVRAEVFKEVDQTVATMFHETETIHDK
                     IGITQPAFAIADKLKLSPVADLVKAIGINEKFLFISQLFNDDSIRYQSAIDKLNSSAN
                     FAEAYMYFDSEITSGNQIDKNSDACKQFVDLLQRRFM"
     gene            46667..48865
                     /locus_tag="PKY09_02550"
     CDS             46667..48865
                     /locus_tag="PKY09_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB11764.1"
                     /translation="MKKPILLFTFFLLSVTLFAADQVLFIKDKSNGKPVNEAVVIWIM
                     PSGKSQILLPDNKGRVKLPETKESGFLIEVRCLGYQSQKIEKETSGDTIWLIRNGVNL
                     NEVAVTASYAPAPAMQSAYAIKVIGKETFDNMGAVNVADVLSKQLNMSTGYDQAFGST
                     LTMMGMDMKNIKVLIDGVPVIGRMDGNIDLNQLNLAAVERIEVVEGPMSTAYGTDAIA
                     GAINLITKPQTIRKTTAGASAYYENIGTYNTDLNVSVNFKHAGLKVTAGRNFFEGWNL
                     IDNRRVFEWKPKTQYFGTALLQYTFSKVKVAYQMGLFDEKITDKGPVTVTPFSAYAYD
                     TYFKTLRNNHSVSYDWFLSGKNKINGHVAFSGYDRHKEVFLKDMVALEQSELTGQDFS
                     GETKFYSWNIRSAFNHVDENKNFNYQGGIDFNIENGTGKRISNKAAMQEYAVFASAEY
                     KPFSKLLIRPGLRYALHSEFTIPLIPSLNLKYDLTNIQAIRLSYSRGFRTPGIKELYL
                     DFIDINHNVQGNNNIKPESSDNFQLVYSLLPEFKGIKINSEVKLYYNKVKNLITLATY
                     SDTSNLYTYINIGHFTSQGIEWSNKIDVGSFSVSAGVSYTGTSSFNESLVNDLSWYLQ
                     ANGSVSYHYKPFGLTTSVYYKYNGKVPVYVLDSNGNSLKQTNEDYQLLDATVSKKMWK
                     DRIEIGGGLKNIFNVTDVRRFATGEIHSGGSSSSSLLPGRLFFLKLSVNI"
     gene            48867..49895
                     /locus_tag="PKY09_02555"
     CDS             48867..49895
                     /locus_tag="PKY09_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HNB11765.1"
                     /translation="MKIKVNIFFLLLPITALLFASCEKDNDKAIVLPPIDTTLQSMQI
                     NIGVNYDTVVFVSLTSGVVKSSAVKDYDLAFDASPESKHIYLNTGKYMFAWRTQCTDM
                     MNTDTTGLNWLTDSDNMLGDSTVFGSNTTSSGDVNQQIVIIDRGKFSHIGSDRYRKIQ
                     IVSIDSNTYTIRYSKLNNSDYHEFVVTKDNAYSQVYFSFDNGGQMKNIAPPKDQWDIM
                     FTRYIHTYWDEPLQFRYYSVNGVLSNRWNNITCAILKKDSLPGYMSFENFNHNDISSY
                     PFYSTGNIIGFEWKYFNFNDNKYYITPDIYYILKDKNDQYYKIKFYDFYNTTGVRGYP
                     AFCYQRIL"
     gene            49916..>50666
                     /locus_tag="PKY09_02560"
     CDS             49916..>50666
                     /locus_tag="PKY09_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11766.1"
                     /translation="MNKFYSIIKLSVALFMPLLGFSQMSETVTINPGYTNQTFYSMAN
                     ATVSTIDNTDWDLAFQISGFEAAILINSKNNVKLFKANKDASEWATMTAADTTGMYYS
                     ELLNNEAHMFNGAFNTTADTSNMFDLGWGEYDLGSHAVMGDSVYFIKLNSNTVKKIWI
                     ESLINATYNFRIADLDGSNEIQRSLQKLSYPAKNFGYYSIANDQFLDREPTKDSWDLC
                     FQQYVDHFINYKVTGVLTNDSVQSVKAYPVND"
BASE COUNT        15977 a         9190 c         9432 g        15767 t
ORIGIN      
        1 cgattcgcat aaaatctgaa agccgttaca aacgcctaaa acatatcctc cttttgcagc
       61 aaactccatc acctcctgca tgataggaga aaaacgtgca attgctcccg aacgtaaata
      121 gtcgccataa gaaaagcctc cgggaagaac aataaaatcg cattcgcgaa gtgaatgttc
      181 tttatgccat aatttttcaa ccggttgttt caatatgttg cgcaatacaa ataccatgtc
      241 ctcatcacaa ttggaacccg gaaatactac aacgccaaat ttcatgtttg agttttttac
      301 tgactacaaa agtaacgata aaaagaactg ctttaacaat cttttttaga gatgtttatt
      361 gagtaaatta aattcaagaa gtttcttgaa tgatttttcg cgtactgttt tatgcaatgc
      421 ctgaatgtgt ttggtggcat gtgtgtcggt accaatcagg tcaacccatt ctttttctat
      481 aagtttttct gcaatgcgtt tggcatcggg accatagtat ccgccaagtg agtttacatt
      541 tatctgcaac aaaacacctt gctcgcgcag actgccgtaa aaatcaaaat tatttcccca
      601 aaaaggataa cgctccggat gtgccattat gggtttgtag ccgagcactt gcattctgaa
      661 aattacttgt aatagattat cgggtgcgtt tacatagctg atttcaaaaa gcaggtggtt
      721 gttgttgcca atggtcaaca atttttcttc ttctaactta tgtataaatc catcgtcaag
      781 ataatattcg gcagcagctt caatggttac aggaatattt tctgccttga ttgcattgcg
      841 cacttcttca agccctgcta aaattgtttc gggcgtattg cgataaaaat cactcatgat
      901 gtgcggtgta gtaataagtt ttgtataacc taatgcatga agttctttaa tcagtgcaag
      961 agagtcttca atcgttttgg ctccatcatc aattccggga ataaggtgac tgtgcatgtc
     1021 ggttttaact tccagaaagt tagctgctgt attttctttg cgtttaaaaa aggagaacat
     1081 aatttatttc ctgtaaatat tgcaacaaaa gtataagaaa atattgcaaa gtgcattgtt
     1141 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttctctatg aaaaaagtgt
     1261 atttggctta tcacctgagt tgaataaggt ttggttttta ttgcaactaa aatttcaaaa
     1321 taatgttaag gctaaaaaga ttgttggaat caatgagatg aaaaatgtca taactgagtg
     1381 tgccagattt taaaaaaggc agaaatacaa tcaacagaca atttatgttg ttgaaaacta
     1441 tgcaattaat tttttaaccc aataaataca ataattcgcc aaacgaaagg tttttattta
     1501 tgtttagtca agtatattag cacagtaatt ttaatctatg aaaccaatgt tcgaatacac
     1561 caaagaaatc ctcaccaagg tgagttttga taaaaagcta ttcaaaaaag aattggttaa
     1621 agccatgcga tggctgaaga gtgatgaaag aaagatgctt atggtttggt gtctggcaac
     1681 tttcggtcac aaatattctg atgtgctgac agaagttttc agaaaggtta cgcatcaggg
     1741 atagtttctg ttattttttc tgatttaaaa aatcaaggtt acgcatcaga cctttaaagc
     1801 ctgtacgatt taatgcagag tctttaaatt tttttttaaa aatttcttct gttagttctt
     1861 cccagtcatt ttgattcatc tccagaaact catgatttgc ttttaggtct tcatgctgat
     1921 gcggttttgc aaagcgattc cacgggcaca catcctgaca aatatcgcat ccaaacatcc
     1981 agtttttaaa ttggttctta aaagaaacag gaatttcgtt tttcaattca atagtaagat
     2041 atgaaataca tttactgcca tcaacaacat gcggcattac aatagcatct gtagggcagg
     2101 catcaataca ttttgtgcaa gtaccacaat aatcttttat tggagcatcg gcatcgagtt
     2161 caatatcaag aatcagttct gcaataaaat accaggaacc tgattgcgga ttgataagat
     2221 tattattttt tcctatccaa cccaatccgc ttttacgtgc ccaggctctg tctaacacag
     2281 gtgcagagtc aacaaaaacg cgtccatcaa ttgctcctat atgctgtcgc atgtgttcga
     2341 gtagttgaaa gagtttttct ttaattacaa aatgatagtc tgttccatag gcgtatttag
     2401 aaatctgagg cgcctctggg ttttgttttt tttcaggata gtaattaaaa agcagggaga
     2461 tgacactctt ggagccttca actaattttg ttgggtctaa acgcttgtcg aaattgcgtt
     2521 ccatgtacga catggtgccg tgcatatttg ctttaagcca ttgttcgagt cgtggtgctt
     2581 cttcttccag aaaaccggct ttagagatac cacaatgtga gaagcctaac tttaatgctt
     2641 cggctttaac ccatgctgtt gtctggctgc ggttcattgt tttttaagac tcagagtcaa
     2701 acaatgttcc cctctgcttt aaagaatttt tgccaagatg tttaaaagct ttttctgttg
     2761 cttcgcgtcc gcgtggtgta cgcatcaaaa aaccttccat aattaaaaaa ggctcataca
     2821 cttcttcaat agtgcctgct tcttcaccaa cagcagtggc tatggtgttc agtcctaccg
     2881 gtcctccttt aaatttttcg ataatggcat taagaattct gttgtccatt tcgtctaaac
     2941 catcaatgtc aacattgaga gcctgaagtc cgtagcgtgc aatttcaata tttatggttc
     3001 cattaccttt tatctgtgca aaatcacgaa tgcgtctcag cagtgcattg gcaatacgag
     3061 gagtaccacg gcttctacgg gcaatctcaa aggcagcttc ttcatcaata gctgttttca
     3121 gaatagatgc agagcgcatt acaatgtctt ctaataatcg tgcatcgtaa tattcaaggc
     3181 gcgagttgat gccaaaccgt gcacgcaatg gcgaagtaag caatccggaa cgtgtagtag
     3241 caccgatgag tgtaaaagga tttattttaa gctgaaccga gcgtgcatgc ggtccgcttt
     3301 caatcatgat atcaatttta taatcttcca tggcagaata aagatattct tccacaatag
     3361 gacttaaacg gtgaatctca tcaataaaca acacatcgtt agcttgcagg ttggtgagta
     3421 agcctgcaag gtcgccaggt ttgtcaagta caggccctga agtaactttt atttttacgt
     3481 taagttcatt tgcaataatg ttggctaatg ttgttttgcc caaaccggga ggaccatgga
     3541 gtaaaacatg atcaagcgct tcaccacgct gcacagctgc ttttacaaat acagtaaggt
     3601 tttctgttac cttgcgctga cctgcaaaat cattgaagct taacggcctt aatgcccgtt
     3661 ctatttcttt ttcggctgta cttaaatttt cacttgccgg atcaagattt ttattgatac
     3721 tcatgtacca caaaagtaaa atttttggga gacatagaaa gcatataagt tgcctaaatc
     3781 ctcaattcac tttgtgccgt atgatgtaac cggtctttct aaaattgtgg tatttatcag
     3841 acatttgaaa caaaatggca aaaagatgaa gagggaatag taaatgaaac agagatttat
     3901 tatttttaga caacgtattt ttatcagaaa acgtcttgat aatattgttg ttatgaaaag
     3961 tataattgca atacttattt ttattctgct ctgtaataca ggtcaggcaa ctcatattat
     4021 gggtggcgag tttacctata cgcttatagg aagtgtacca cagcaacaga aaaaggttta
     4081 caagataagt ctcaattatt accgcaactg cgatacttcg gctgcacaat ttcaaacgtt
     4141 ttatgatctt tatatttgtt atgactcttc caacaccaac tataaaagat tatatacgca
     4201 aatttcgctc gataagataa acacacaacc ggtaacactt ccggtattag acacaacttg
     4261 ttattataac aacaattggt gtgtagaaaa agcatactat gaagcaacag taatattgtc
     4321 ggaggataca acctggcact tgtttttaaa tgatggggca cgaaatgtaa gtatcaataa
     4381 tctgattcag cctgatagca caggtatatt tttttacagc atgattccgg caggtatttc
     4441 taactccaca ccacagtttt cgagtttgcc cacacctttc atctgtaatt tggatacagt
     4501 tcagttaagt aacctggcat ttgacagtga tggagatagt ttggcctata aattagtgag
     4561 gccttatggc aatttaggtg taaacaattt aatgacctat caggatatta atttaaactt
     4621 atacttttta tttccgttac ctacggtcat ttacagcccg ggttatagtt acacaagtcc
     4681 ttttggtgca ggaggctttt gtagtttaga ttcacagacc ggactgctga ccatctcttc
     4741 acccaatcag ggattgtatg ttttgactat tgaagtagaa gagtacagaa atggagcact
     4801 gctttccgga acaaggcacg acatgcaggt gatagttgcg gcatgctcct ataacacggc
     4861 accacaatta acttcacctg ttgttttaaa ctatcagatt gatgagggtg attcactttg
     4921 ttttccactt acatttgctg acgctgaaaa caatcaaata aacatttcgg caacaggcaa
     4981 tctttttgat gcagcactca acaatccgcc tgccttgttt gttgtgaata cagcaccaaa
     5041 caataataca gcacagtttt gctggaaaac aatttgtgga caggcagctc cgtttcccta
     5101 tatgtttaca gtagcaataa cagataatgg ttgtccttca aaaacaaaca cctatgtttt
     5161 tactatactc gtaaaatcaa tttctcacaa ccaacttcca tccattacgc tgagtatgtt
     5221 tcctgataca gcaatctgta caggaacagc cattcggttt aatgctacta cacagtttgc
     5281 aggaaataat ccaacatacc aatggtatat taacggacaa gcaatttcta ataattatcc
     5341 tgactatatt acgaatcaac ttaacaatgg tgacagcgtc tcggtgcaac tcatcagtaa
     5401 ttctatttgc gtaatcgatt ccggtgcaat ttccaataca caagttgcaa cagtgttgca
     5461 gcctaccgtt gcttcattca ttacaaacac tgtttcggta aatagcatca atgcgctaca
     5521 acagtttacc aatctatcgg tgggcaacac ctataattac tggaactttg gagacagtac
     5581 acagtctgtt gatataagtc cacaacatgt ttatgtaggc agtggtattt atctggtgca
     5641 actcataact gttagtagtg atggctgccg tgatacaaca gaagtaccgg tagatatcaa
     5701 aacaacaatg agttttttta ttccctcctc atttacacct gatgcaaatg gactgaatga
     5761 ttggtttgct ccaattggag cagaaatgca ggattatacg cttcagattt ttaaccgttg
     5821 gggacaattg cttttttcag aaacaggaaa gccacaatgg aatggccgtt ttaaaaatca
     5881 actgctgcct ttaggaacgt acatctattc cattcggatg aacaatccaa cagatcccat
     5941 tattaaaacc ggaacagtaa tgttgttgcg atgaacattt ttattttaca tggattgcgc
     6001 aaatgtagat ttgaaaaaaa caacattttg tttccacaca aagcgtataa gagcacatca
     6061 agttctacta ctaattgtac taacagtaac attttgataa gttgacgata aacaactgct
     6121 aagcatgata ccactattta tttttgtatg acaaaaaagt aattatgttg aagcgttttg
     6181 tttgtataat attgtcacta ttgactttta caggtttgaa agcacaggat aataagacag
     6241 aaaagaaaga cagtattgaa catcgcattt tgcttattcc ctacgaccca cgtttttatt
     6301 tgtctgatgc cgataaagat attgctgaag aaactcgaaa agaccccgaa acaatacgta
     6361 attattttca caatcgcagc gaatggttta cctggcgtga attaaagaga cattatccta
     6421 caatttcact cttacagaac gacaccgttc ctgcctatat tcagtcagca ggcgatttgt
     6481 tcagccaaac agcatttgat tatgccacac ctattacgcg atcacccgaa agtttgatga
     6541 atgcaacatc agataaaaca cttaccgatt cgcgtacagc aaccaattat ctgaatactg
     6601 atatacacaa ccgctacatg aaagccgata ttcagaataa aaaagtatta gccgatttga
     6661 gtgagcgatt caatacagac ctttttgttt ttcttacaca atttgagatt aaaaccaatt
     6721 ataaaaactg cttggatatt gccaataaag tatatgaacg tgaaatcaga cttcatttta
     6781 ccatctatga taaaacagga aaactaatca atggaaacta tgccattgct gtaattccat
     6841 caaacgtaaa tcagatggac gaaattattt taaagtgttt tcctttgttg gcaaaaggga
     6901 ttgcagcatc attatgaggt aatattttta attatttgtt atccggatgt tatattttac
     6961 agcctttgtt ctacattcgc aacttcaatt tattcaaaca tgagaaaaag tttaacacta
     7021 agcacaatat ttgtaattct gctaggggca caaacgttgg caagtaattt ctggactgtt
     7081 aagtcagaaa cgcaagaaat gctatcaggc aaacgtcaga ttgttcctga taaatatatg
     7141 gtttatacac ttgatgtaca aggattgaaa aatcaattgc tcaatgcacc attagagttt
     7201 accatggcag caaagcaatc aacggttgtt attacattac ctatgcccga tggcagttgg
     7261 aatagcttct ctatggtaga gtcgcccatt atgcaacctg aactggcagc taaatatcca
     7321 tttattaaaa catattcagg cagtggaatt aacaaccaac cttcatgggt tcgtatagat
     7381 tttacacaat ggggatttca tgcattaatt agaacagcag aaaacgatat ttatattgac
     7441 ccttattcca tgcaaacaac ctctgattat ttagtttact tcagaaaaga ttttacacaa
     7501 tcgcaaagta gtatggtttg tgaatttgac gacttacaac aaggtcaagg tacaaataag
     7561 gcattaacaa actctttgcc tgctatgaac cgttccatag gaacaaattt acgtacctat
     7621 cgtttggcat tggcctgcac cggtgagtat gctgcatatt atggcggaac aaaagcaggc
     7681 gcattgtcag gaatggtaac atctgtcaat cgtgttacag gtgtttatga atcggaagta
     7741 gatgtcagac ttaatttaat tgctaacact gatactttaa tttttcttga tgccaacacc
     7801 gacccttata ccaataacaa tggcggaagt atgctggggc aaaatcaatc cgtagtaaac
     7861 ttttttattg gtacccccaa ttacgatatt ggtcatgttt ttagtactgg tggcggaggc
     7921 attgcctact taggttgtat ctgtaaaact gcagataagg ccaagggtgt gacaggatca
     7981 tctgctccta tcaatgaccc ttttaatatt gactatgttg ctcatgaaat gggacatcag
     8041 tttgggggca atcacacatt taatagtgta acaggaagtt gccagggtaa ccgagaagca
     8101 agtgcagcat ttgaacccgg tagcggtgta accattatgg gttatgcggg tatttgtggt
     8161 tctgatgatt tggctgcaca cagtatagct tattttcata caaaaagttt tgatgagatt
     8221 gttgattatt caaccttgtc aacaggcaac tcttgtcctg taaatactat cacgtttaac
     8281 aatgcaccta ctgttagtgg cggaggcata tttaatgttc ctgtaggcac ggcatttaaa
     8341 ttagatggct atggcagcga ccctgatggt gattccataa ctttcagttg ggagcagttt
     8401 gatttgggtc ctgcaggcgc atggagttta ccaacaggta atgcaccaat gtatcgtagc
     8461 tatgctcctt caacttcagc actacgtttg tttccaaaac tttctgtatt acttaatcag
     8521 gtaaatgcca aaggtgagtt gataacatct tatgcccgct ctttacattt cagacttaca
     8581 gcaagagata acaaacccaa tggcggaggc gtaacttata acgatacaat tacaacagta
     8641 aatattgttc agactgctgc accttttgcc attacctatc caaatgtaac aggtatatca
     8701 tggcctgctt taagtacaca aacagttaca tgggaagtag gaggcacaga tgctgcacca
     8761 tttaatgagc agtttgtaaa tatctacctt tcgattaata atggaacaac ctttcccatt
     8821 acaattgcaa ccaatgttgc caacaacggc agctatactt ttactgttcc caataaccaa
     8881 tctaatattt gcagaatgtg ggttgaaggt gctagtacag gttctatatt ttttgatatt
     8941 aacaacaaaa catttaccat tacagcacct gttggaatta atgaagtcaa cagcttgagt
     9001 cagcttacca cctatcctaa tcctgcggac aatgagttgt tggtttatat aggtaacagt
     9061 aatcatggcg aattgagttt acgtttattc agtgtcaacg gacaacttgt gttgtcacaa
     9121 acaatagcag ctaaagatgc tgcagcacag gttgcgttga atgtagcaca actgcctgca
     9181 ggtttatatt ttctgaaggc agaaacagaa aacggaacag cagaacgtaa aataattatt
     9241 aagtaaaata ctttgatagt aattcaaggg ctgttcacaa aaacagccct ttttttatgt
     9301 tttaattttc cgacttaagt aaagttcaaa ttaactactt tcgcatatca cataaaacaa
     9361 aaaaggtgaa gccatttttc aaagctgtat tcacgttctt atttaccatt actatattgc
     9421 attctgcaga tgcacaattt tatcagggct cgcagttgtc ttttggaaaa aaccgtgtac
     9481 aatacaatga tttttactgg acattttaca gatttaaaaa ctacgatgtt tataattatg
     9541 taggaggaaa agaattagcc acctatgttg ccgagcaggc acagaatgaa atggatgcca
     9601 tggagcgcac tttggattat cgaagttcgt cacgttttca gatagtggtt tttaataaac
     9661 taagcgatct taaacagaca aatatcggac ttgaattaga tgatcagacg tataatattg
     9721 gtggaatgac aaaggtaatt gggaataagg ttctcattta ttttgatggt aatcatcagc
     9781 atttacaaga acagattcgt gccggcatgg ccagagcctt gcttgaacag ttgttttatg
     9841 gtggaaacat cagagagcgg ttacaagctt ctattctcct aaaccttcca cagtggtaca
     9901 cacagggctt ggtaacctat ctttcaaagg gatggacatc agaagatgat aacaaactgc
     9961 gtgattacat catgactgaa aaaaaaccgg tcttgaataa actcatagag cgtgatgaaa
    10021 tttttgcagg tcagtcctta tggcactata tcatcacaac ttatggagaa gcatcattaa
    10081 gtaatttggt ttatatgaca cgcatcaacc gcagtcttga aagcggtttt gtttttgtgc
    10141 ttggtgttcg tttgaaacaa ctttctgcag actggttaag ttatcacaca aaaaaatatc
    10201 aggaatttga aaagaacaaa ctccctattg cagcaatacc attaaaaatt aaagaaaaga
    10261 acaagctaat ctatcaggta aaatcagacc cttttggaaa cagagtaagt tatgtaacca
    10321 ataaacgtgg tcgctacaag gttaaaatcg ctgatgcgaa aaccggtaaa cgtacttgcg
    10381 ctgatagagg aggatatgtt tcactgacaa cagaaaacga ccaatcattt cctgttacag
    10441 cctggcatcc gtcaggaaaa atattagcta ttgttaaaga gcaaaaagga aatattattc
    10501 tgaatttgtt taatacagaa acaaaaaagt cagacttttc agagataatg agtttttcaa
    10561 aaattctgag catgaactat tctcctgatg ggtcaaagct attgatgtct gctgtacaga
    10621 aagggcaaag tgatatttat gtatttaatg tgcgtggccg aaatttcgat cagattacaa
    10681 acgatgtgta tgatgacttg tatccacaat atgatatttc aggcagcaaa ataatttttt
    10741 cttcaaacag aagtaatgat accttaggtg ttgacgttaa acaaaatccg ccacagaaca
    10801 attttgatct ttttatggca gacctctaca atagagaggg aaatttattg cgtataacac
    10861 attcaccgga agatgagact ttccctgtaa tgagtgattc gctgcggata tcttatctgt
    10921 cagataaaaa tggagtcatc aacagatttg tatcagagat ggatagcacc attagtttta
    10981 ttgatacaac agaacactac cgatattttt tcagacatta tgctttaacc aatagcaaca
    11041 gaaatattct ggaacaggat ttttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnttcaa atcaaagaag aagaaagaag aaccgataga aaataaatct gtggctacag
    11221 atactactaa aattgtgcag gaacaagtaa agttgcaacc ggatagcaat ggagtagaat
    11281 taacgccaga ctctgcaatt ttcagaatgc caaagcaacg caactacgat atttcttttt
    11341 catctgacta tatacttacg cagctcgaca acagtttatt caacactaca tatcaacctt
    11401 acactggtgg tgccgtttat tttgatcccg gaatgaatgc attgttcaag attggaatga
    11461 gcgacctgat gaatgattac aaaatcagtg gagcgtttcg cattggattc gatttcaata
    11521 gcaatgaata tttgttgatg attgaatctt tgaaaaaaag aattgataag cagatacttt
    11581 tttcgcgtca gtcaaaattg ttttctgttt cagatctcga tgtgttacgt acacatacct
    11641 ataatttaaa atattcaggt aagtatccat tcagtgaaat ttctgcagtt aaaggaacta
    11701 ttgacgcacg aatggataaa actatttatg ttgcaacaga gaaagcacaa ttagagaaaa
    11761 gtgatataat aaattattgg gcaagcataa aaggagagta tatttttgac aacaccatca
    11821 ataaagcatt gaatctttat aatggtacac gttttaaaat ttttgctgaa gcgtttcgta
    11881 aaatcaacaa gcccgaaaaa atggtttata tcattggtgc agattttcgc cactacgtta
    11941 aagtacacag acaaattatc tgggctaacc gtttatcggc aagcacttca ttaggcaagc
    12001 aaaaattgat ttattatttg ggttctcagg ataatgttat tgttcccggt aataatttta
    12061 ataatgaaat tagtgttgac aagaacgaag agtatgtgtt tcagacatta gcttcaccca
    12121 tgcgtggttt taaacaaaat atccgtaacg gcagcagctt tgcattgttc aattcggaat
    12181 tgcgtattcc cgtatttcaa tatttgctca acagaccaat ccgctcagac ctgattcgta
    12241 attttcaggc agtagtattt ttcgatgcag ggacggcatg gtcaggcgaa tcaccatttt
    12301 ctgacaagaa cggattgaat gaagaagtga ttggaggtgg tggtagcaac ccggtaacag
    12361 ttactgtgat taaacaaata caacccatag ttggtgctta tggcttcgga tttcgtagtc
    12421 gcatatttgg ttattttatt cgtgccgact ggggttttgg aattgacgac cgtcaggtgc
    12481 agaaaccatt attcaatctt gctcttggtc ttgatttctg attatcgcaa aacaaatgtg
    12541 cgatagctgt ctaattttat cagaataaat aaaaggattg taaatcccca taaggatgag
    12601 ccgccataac tgaaaaaagg cagaggtatt cctattacgg gcatcagacc aattgtcata
    12661 ccaacattta ccataaagtg aaagaatatt actccggcaa caccatagcc atagattctt
    12721 gcaaaagctg acttctgccg ctcggcaatg aagataatcc tatagagcaa tgccagaaac
    12781 aataacacaa cagttgttgt accaacaaat ccccattctt cgccaacagt acaaaaaata
    12841 aaatcagtac tttgttcggg cacaaaatta tatttggttt gtgttccttt taagaaacct
    12901 tttcctgtca acccacccga accaattgca attttacttt gatttacatt gtaaccggca
    12961 cctttaagat cgtcttccat tcctaaaaac acctgaattc gttttttctg atggggttct
    13021 aaaacatgat taaacacatg atcaacactt acaacgacac caatacaaat ggcgactaat
    13081 cctgcaacaa caaaaaaagt tttccattta cgaaataata aaacaattat tgttgcaagt
    13141 atcagcagcg aggtgacgag tatcattttt ggtactaata atgaaaccac aaacaatact
    13201 gcagcagaaa taccaatcat cagcaaccat cccggaataa tttcttcacg ataaagtaca
    13261 atacaaaaag atagaaatac caaagccaca ccggtttcat tttctaatag aatgagtaga
    13321 agtggaaaaa agaaaattgc tactgcagaa atacgtgttc gccaggtgtt tttctttaat
    13381 cccagagggc ttaaaaattt tgcaatcatc attgccgtag caaattttgc aaactctgca
    13441 ggttgaattc caaagtcgcc aaagcggaac cacgacttgc tgcctgcaac tgtatgaccg
    13501 aaaaaaagta cagcaatcat agaaattata ctgattgcat aaataactcc ggcaaaggca
    13561 tcataaaatt ttccatctat tatcagaata aaaaaagcaa ttacaagtga tgtcagtatc
    13621 cacatcattt gtttgccata gttttgtgaa aagtcggtta ttacactatg ttcctcatta
    13681 taaactgctg caaaaatgtt taaccagccg gcaataacca taaccaaata aagcagcacc
    13741 agcaaccagt ctatattctg aaaaatactt ttctgcttcc tcatttttta gtcatctaaa
    13801 acagttgagt cgtttgccat aggcactatc actgcatcta acattcgttt ttcaagatcc
    13861 ggacgtttta tggttctcaa taaatatttc tgaaccatca aacttgcaat tggtgcagca
    13921 aaatgtgcac cctgacccgc attttcaact aaaacagcaa cagcaatttt tggattttcg
    13981 cgcggagcaa agcagaaaaa tacagagtgg ttttttccat gtggattttg tgccgttcct
    14041 gttttaccac acattaccaa actgtcaaca cgcgaaccac gggctgttcc acgttcaaaa
    14101 acccattcca ttccgtcttg tatggcatta aaatattttg gatcaaccat cgtataattt
    14161 ttctttttaa aaagctcagg cagataatgt ttgtatccga tttcttttac aatatgaggc
    14221 acataataat atcctttatt agctacaatg caagctgcat tagccatttg caatggtgta
    14281 atacccaatt caccttgtcc tattgctaac gaaataatag ttgatgattt ccatcgtcct
    14341 tttccaaaga aacgattgta gtaatcaacc gttggaacat tgcctcgcaa ctcatggggc
    14401 atatccacat caattttttt accaatgcca aagcttagaa cataatttcg ccatgcatca
    14461 aaagcatctt cagttcggtt gtattttctg ttgtctatta cccctcgaaa agtctggcag
    14521 taataggagt tgcacgaaac ggcaactgca gtacgtaaat caagaggaga actgtgtggg
    14581 tggcagtgaa caaaaatatt tccaacccta taaccacctg cgcagctgaa ataagtttca
    14641 ggagtgatta cctgctcctg caaaccaatg agagagttaa ttattttaaa tgttgaaccg
    14701 ggaggataat aggccatcat ggcacgatta aaaagtggtt tatcctcatc ttttaataaa
    14761 cggccatagt tgcgtgaacg ctctcttccc accataaggt taggattgta ggtagggctg
    14821 ttcaccatag caagtatttc acctgttgaa ggatcaatag caacggcact tcctctttta
    14881 ttctgaaaca ataactctgc atactcctgt aattctgcgt ctaatgaaag ttttaagttt
    14941 tcaccggata ctgcaagcgt atcatatttt ccttctttaa aacgtccttt ctcacgattg
    15001 aaattatcaa ccatcatata tcgtgtaccg cgtctgcctc ttaattcttt ttcataactc
    15061 tgctcaatac cactgatgcc aatataatcg cccatttggt aatagttgtt ttttgcagtc
    15121 acattttcat ctacctcacc aagataaccc aacaagtgtg ctgcggtgtt gtaaggatat
    15181 tttctgatgg ttcgtggttg aacactaaag cctttgtatt taaaaagctt ttcctgaagt
    15241 gttccgtaag tttctgctga aatttgtttt tcaaaaattg atgaacgata gggagaatag
    15301 ttttttgctt tctgaaattt ctttctaaaa tcttcaatgc ttatttttat gatggaacaa
    15361 aattctgctg tgtccatttc tttgacttcg cgtggtgtta tcattaagtc atagacggtc
    15421 tgattaaata ccaacagttt tccatttctg tcataaacca atcctcttga aggatagtca
    15481 attattctgc gaagaacatt ttcatctgcc gacagcttat atttatcttc aacaacctgc
    15541 aaatagaaca aacgcccgat ataggtaaaa gccaccaatg aaatggtaat gagtaaaact
    15601 aggcgttgtg tgctatgcga ggttttcaaa atttaaaatt ttagcgttgc acttttgttt
    15661 tgtcgaataa caacaaaatg ataaaaataa ttacaacaga ggcaatggta gataaacata
    15721 ctctgaggaa tgttgctccg aaatcgttaa atcgaaatac ttctgtatag aacaaaaaca
    15781 ggtgatgcaa aaaaatactg ataaataaat aatacaccat ccacaaaatg cccatttgct
    15841 ttaatgagat tacagcatct gagtcatagc catcacgtgg ggcaagtagc ttgatgatgt
    15901 agggacgcat aaaaccaaga aacaccgatg cagcagcgtg catacctagt gtatcagaaa
    15961 aaatatctac acttattcct gtgataaaag caagaataag caacaatatt tttggaatac
    16021 gaacgggaag caacaacaga aaaagcggat acacaaaagg attgatatat cctgcaaatc
    16081 ggattttgtt aagcagcact acctgcaaca atatcagcag aaaaaaacgt aagacagatt
    16141 taattacctc gggcagcatc tttttgtttt tcttctaatt gtttacgttc gtttttcagc
    16201 aggttgttca ccacatacac atacgacaca tcattaaagc ttgtgctgag tttaagaata
    16261 attctatagg agttgcttcc tgcatcaatg tcgaaagatt ttactgtacc caccataata
    16321 ccctcaggat aatagggcga aaaggttgta gtcaccaacg tgtcgccaac tttaaaagga
    16381 acgtgtcgag gaatgtcaat tacagttgcc tctgtgggag aattatcgct atcccaaacc
    16441 attgagccat tatagttgtt gtttttaaaa cgtacactca ccttgctttg tgtgtgaaga
    16501 aatgaggtaa cagtgctgta atgtgccgac acattcttaa taataccaat aattccatga
    16561 cctgtaatga cacccatttc aggtgcaatg ccggcatcgg aacctttgtt aagcgtcata
    16621 taattgttgc gcctgtttgt actgttgtta atgattttag cctcaatata ggaatattca
    16681 agattatgaa tggtatccac tttatgattt cttacaactg ttgtgtcgaa gtacgatgca
    16741 ggaagcaagg tgcgtaagtg tgcattttct tcagcaagcc ttttgttgtt ttctacaaga
    16801 ttcaaataat cggttacaga acttatcgtg gaatatactg atgcaacaac tttattggtt
    16861 gagtttagtg tggctgcatt ctgataagag ttattctgtg caatgaggaa gaagcaaact
    16921 gcctcaagca gtataaacaa tatgaaaata taattccggc ttagaaacag gaataaatta
    16981 cgcataaact ataatttgca gaataaaacc caacctttac aaaggtgcaa aagtaatttt
    17041 ctaagcatta ttgaccactg aaaagtttta aacgaggtta ttaatattgt ctgacaaaaa
    17101 tcaccattaa ccaaacacct ttaatgtgct tggcgttgcc tattttcgca atctaaattt
    17161 taatagtgcc gtattggaaa aaaagttaag cgaattaaag cccggtgagg caggggttat
    17221 acagaatttc agtgacgaag agataaaact taagctgatg gagatgggtt gtattcccgg
    17281 tgaaacggta ttgatggaac gagttgctcc attgggagat ccaatggcaa ttaaagttgc
    17341 aggctatcag cttagtttaa gaaaaaatga ggcagaaaca gtatgggtga gtacattata
    17401 aatagaagga gaatataagt gagcgaagtt gttctaaaag ttgcattggt tggtaacccc
    17461 aatagtggta aaagcacttt gtttaatgta ttgacaggcc tcaatcaaaa ggtcgccaac
    17521 tttcccggtg tgacagttga gaaacatgta ggctacttta gcttgcgcaa tgcatttgaa
    17581 ggtgttgatt ataaagttga actaacagat ttaccaggca tttatagctt ataccctaaa
    17641 acacttgatg agcaaatacc atacaaagta ctaaccgacc cgcaagaccc atctttcccc
    17701 gacagaataa ttgttattgc tgatgcatct aacctaaaac gcagtttgtt tttatgctct
    17761 cagattattg accttaaaat tcctgttgtg ttggtgctta acatgatgga cctggctgtt
    17821 cacaaaggtc tgttgcccga tgtaaaaaaa ttagaacagg cattaggcat caaagttatt
    17881 ccagccattg tacgtcagaa aacaggtatt gaagaaatca agaaagcagt actgcatact
    17941 attcctttgc ccgaaaacga tattatgcct gcaacatcgc tggcacctga actgattcag
    18001 gaaatcaaac atagttttcc ggtaaaaagt gattatgctg catatctgct tgcaggtaat
    18061 tatgataagg tggatatcag ttttgtaggt aatgatggta aacaaaagtt agacacactt
    18121 ttacaacaac atcagtttgg gcaaaaagcc atgcaggcta ctgaaacatt agaacgttat
    18181 aaaactattt ctgttctact taaaagttgt gtcagcgaac cggcatcagt gcgtaaatcc
    18241 ctgacccgta ccgttgataa tctggttacc cacagaatat ggggttatgg aattttcttg
    18301 gctatactct ttgtaatttt tcagtttatc ttcaatgtgg cacaatttcc gatggatttt
    18361 attgacggaa cctttgccga tttttctcaa aaagtaagcg aattgcttcc taagggtgta
    18421 ttgaatgatc ttatcgtaaa tggtattctt gcaggtcttg gtggcatcat catatttgtt
    18481 ccacagatag ctttattatt tttattcatt actattctcg aagataccgg ctatatggca
    18541 cgggtgagct ttataatgga taagttacta cgaccatttg gattaaacgg caggtctgta
    18601 attccattga tgagtggtgt ggcatgtgcc gttcctgcca ttcttgcaac ccggaccatt
    18661 caaagttgga aggagcgtat agtaacaatt atggtaacac cactgatgag ttgttcggca
    18721 cgactacctg tttatacctt actcatttca ttggtaattc ctcgatatta tgtttttgga
    18781 tttatcaatt ctcaggggtt ggtgatgatg cttatgtatc ttatcggatt tgttgcagcc
    18841 atattttctg catggttatt taagatgttt gttaaggcca agcagcgtga tttttttatc
    18901 atggaattac cggtttaccg tttgccacgt tggaaaaata tcttctttgt aattttcgat
    18961 aaagtaaaaa tatttttgtt cgatgcggga aagattatca ttgccatttc cattgtgctt
    19021 tggtttttat catctcgcgg acccggaaaa gaatatgctg aaatagaaaa gttatatgag
    19081 gctaaggcag aacttccgga aacaactcag gacgaactga atcaattgca ggctcagaaa
    19141 ttggaacatt cctatgccgg atggacagga aaaattattg aacctgcaat tagaccgttg
    19201 ggtttcgact ggaaattagg aattgctttg gtgacatcat ttgcagctcg tgaagtgttt
    19261 gttggaacaa tgtcaaccat ttacagtgta ggtagtaata gtgaaaattt aactatcaga
    19321 gaaaagatga tgacggaaaa acgaccggat ggaactccgc tttatacatt agctgttggc
    19381 atttcactga tgttgtttta tgcttttgcc atgcaatgta tgagtacact tgccgttgtt
    19441 aaacgcgaaa caggaacatg gaaatggcca atcattcagt ttgtatatat gagtgcttta
    19501 gcatgggtat caagttttgc tgcttttcag ttactaagtt aatttattat ttttcgaccg
    19561 aaataaatta tggcaaaagt tactcttgaa tttatagaag aagcattaaa aaagtatgtt
    19621 ccgcataagg caataaggta ttgcatacaa tggatagaag aacatcagat caatcttaaa
    19681 attacaagaa gccgaaacag caagtatggt gattaccggc cgccacaaag aggattcggt
    19741 catcgtattt ctatcaatca cgatcttaat ccgtatgctt ttctcatcac attcattcat
    19801 gaagtagctc atctcaatca gtggaaacgt aaaaagcaaa ttatcattcc gcacggaaag
    19861 gactggaaag aagagtatcg taaattgatg attcccattc tgcgcgagaa tatttttccg
    19921 cccgatgttg ttgcggcttt aaataattac atgacaaatc catctgccac aagctgttcg
    19981 gaccatgatt tgctaagggc actacgcaac tatgacagag aagaagataa atggattaca
    20041 ctggaagaat tgaattttgg cgtgaagttt aaaatcagaa caggtcgtac ttttatcaaa
    20101 aaagaaaagc ttcgcaaaaa ttatagttgt attgactgtc gttccaaaag catttacttt
    20161 atcaatccca tcacggaagt gatgcccgta gatcattgaa caaagaaacg atcgggaaca
    20221 ttaatcagat aaactttttt gcgcgcacgg gtaatggctg tatatagcca gcgcatgtaa
    20281 tcaacaccat acatttcttc tgtcataaag ccgggatcaa taaacactgt ttcccattgc
    20341 ccaccttgtg ctttgtgacc cgtaatagca taggcatatt ttacctgtaa tgcattgaga
    20401 tatggatctt cacggagttt acgcatgttt acttttccat ccgccttgcg ataatgattt
    20461 cttacctcat caaataattt cttatactca tcataagtca aagctggcgc ctcactgttt
    20521 aaagtatcaa gcagtaattt tacttcaagt tttgttgatt tttcatcaga taattctatt
    20581 gttgcatttg caaagcgaaa cccaaaatat tcttcctgcc tttttaaacg aatcaattta
    20641 atttcatcac cattggcaat aaatgcattg tctttgtctg attcatgcca atagtaatta
    20701 ttttttacaa ccatcagcct ttcgtttttt tctactacct cttcaaagcc ataaaaagtt
    20761 tttctgataa actcattgta acggtttgcc tgcttattac ttctgcacaa tacagttact
    20821 gtgctatcat cgcctgatgg gaaatatcct gaaagtactt cagacagttc cgaagatgtt
    20881 acctgtttta catcatcaaa ccctgatgtt ttaagacgag gaaaacgctc ttctctttca
    20941 agcaatagtc gtgtacagtg agcattcgct aatattcccg agtgaagctg ttgtcgcacc
    21001 acctctttta attcatattg ccttacctga agctgacagt tgttttgtag aaaattctga
    21061 tccaaggcag ggctacattg taacccaacg gggggcaatt gcgcagtgtc accaataaaa
    21121 actatggcat tatctgcctg actgaaagca tgatcaatta agtcagaaag taaacttgcc
    21181 cctttaaata aagttttgtc atcttcatta gaaatcattg aggactcgtc aacaataaaa
    21241 atctgctttt tgtatttgtt tggttttacc tttgttttaa tgtcgccatt tttatcgctt
    21301 ttaatttcat ataaatagcg atgaattgtt gaagcatcat caccggtgta atggttaatc
    21361 acttttgctg cccttcctgt cggggcaagc attatcactc tgaaatcata gtgataaagc
    21421 gtttttatca ttgcgttaat taccgttgtt tttcctgttc cggcataacc ttttaaaagg
    21481 aagcatttat atgcagtatt ttcagtaaca aactccgata gttctgcaaa ggcatgtttc
    21541 tgtgcattgg tgggttcaaa tggaaaattc ttcaataaat gattaaaaac agcagcaggc
    21601 ttcaatgttt atcttgtgta attaacggat aaaaatacaa aagagaaagt taataaaata
    21661 agttaggtaa catatgatta tagcaatgat gcaaagagtt gtacttaata aacatatatg
    21721 acaaacattt attatagcaa aataccgaac gctacactct gaattcttaa ctataacccc
    21781 attaatcttt gtactttcgt gcaaaaaaat aaagcaatgc tcagcataga cattcacaca
    21841 catatattgc cggagaaact acctgattgg aaatcgaaat tcggctatgg tggttttatt
    21901 accttagatc atcacaaaag ttgttgtgca aggatgttgc gtgatgatgg cacttttttt
    21961 cgtgagattg aaagtaattg ttgggatgca aagacaagaa tgcatgagtg cgatgtacat
    22021 ggtgttcatg ttcaggtttt gagtactgta cctgtgatgt tttcgtactg gacaaaacct
    22081 gcagatgggc ttgctgtatc agaatttctt aacgatcata ttgtaggcat tattcagcaa
    22141 tatcctaaac gctttgtggg tttgggtact gttccgctgc aagcacctga acttgctata
    22201 aaggaaatgg aaaggtgtaa gaagattggt ctgaaaggaa tacaaatagg ctcgcatgta
    22261 aacgactgga atcttaatga tgtaaaccta ttttcgtttt ttcaggcttg tgcagaaaac
    22321 aatatgtcac tttttgttca cccatgggaa atgatgggtc agaaagaaat gcaacgctac
    22381 tggctgcctt ggttagtggg aatgccagca gaaacatctc gtgctatttg ttcaatgatt
    22441 tttggaggtg tgtttgaaag attaccaaac ttacgtgtag cttttgctca tggtggaggt
    22501 agttttccgt caacattcgg gcgtattaaa catggctttg actgccgtcc cgacttagtt
    22561 gctgtagata atacagcttc accggagaat tatattggta agttttattt tgactcattg
    22621 gtgcatgatg aaaacatgtt gcattacctg attaatttta ccggtgccaa ccgcattgca
    22681 ttaggtagcg actatccttt tccgttaggt gagaatattc ccggaacctt gattaaaaat
    22741 gcaaaactcg atttagaaac taaagaacgt ttacttggag gaacagcgtt ggagtggttg
    22801 gacttatcaa aagaattatt tctgaaataa atttttactt tcattcaatc tcccaaaaca
    22861 ggattaaaat tgttaaagac tccttaaatt ttttttaagg aaactttaaa aaccaaaata
    22921 gtttccatag ttttgacccg ttttttacaa ttatcatgga tataagagaa agaattttag
    22981 agtcgggatt ggagctgttt ctggcacatg gtgtcaggcg tataaccatg gacgatatag
    23041 caaggcattt gtcagtttcg aaaaaaacaa tttacgcatg ctttgatgat aaagatgaaa
    23101 ttgtatctga atgttgtcag ggatatttaa aaaatagccg ttgtgaattt gaacacgttg
    23161 aaacagaaag caaggatgcc atacacgaaa tatttctgac aatggaaaaa ttacaaagca
    23221 tgttcaccac tatcaatcct gcattgtttg aagacttaca aaaattttat ccgcgcgtgt
    23281 ggagcatgta tgtgcagttt aaagataaac atgttgcaga agttattgaa aggaatcttt
    23341 taaaaggtat tgaacaaaaa ctttaccggc cggacatcaa tattaaaata ttagtaaaac
    23401 tgcgtattga agaaattaca ttgggaatga atactacagt ttttccatta aaaagtttca
    23461 taactaagga tgttcagatt gcactgctcg atcattatct gcatggcatt gttaccgtta
    23521 agggacataa actaatcaat aaatataaaa acataacaga cgaagaataa ataacaaccg
    23581 tatgctgaat caaattaaat ttcttacagg attactgttg ctgatatcaa tcaatataca
    23641 ggcgcagcaa tccgataaaa agtcttattc cttttcattg aaacaggcaa tagattttgc
    23701 ttttcaaaat cagactgata ttcaaaacgc agtacttgac cagaaatctg cacaaagcag
    23761 agccaacgaa ttactgggaa ttggtttacc acaaatcagt gcaagtgctg atattacaca
    23821 ttttattgac atcccaacaa cttttgtacc tgctgaattt tttggtggag aaaaaggtag
    23881 ctatgcacca gtacaatttg gtcagccata tagctccact gccggattgt ctgcatcaca
    23941 attattgttt gacggatctt atttagttgg tgtaaaggca tcacgcatgt atgccgattt
    24001 gatgcgaaag caggtaattc agactaaaac agagaaagca gcgcttatca gtaaagcata
    24061 ttataatgtt caggttaaca gtgagcgttt gcagatgctc gatgcaaatg ttaagcgatt
    24121 aaaaaagcag cttgatgaaa tgcaggttat gtatgataac ggttttattg aaaagattga
    24181 tttagaccga atcagtcttg cctataacaa cattgaaacc gagaaagcaa atgctgacag
    24241 attaattgtc cttgcacaaa atctgttgaa attccagatg ggattgaatg ttagtgatga
    24301 gttggttctc actgacaaat tggaagatgt gaaagttgtt gccgacaata acctgaatca
    24361 aaatattgat ataagcaaca gaatagaaat ggatgtgttg aatacaacca aagaattact
    24421 tagagcagat ttaagattaa aaaaatcttc ttacctgcct tcacttgcag cttttgggtc
    24481 atatagtgtt agtgcctcac gcaacgattt tgatattttt gaaaacaaac catggtttcg
    24541 cacttctata atcggtgtaa aacttaacgt gcccattttt gatggctttc aaaaggcagc
    24601 aaaagttaat caggcaagat ttgcattgaa taaaatagag aatcaaatta aaactgccac
    24661 acaaggattt cagttagaag cgcagacagc aaagacaact ttaaccaatg cattaggctc
    24721 tcttgccaat caggaaaaaa acagaacatt agctgctgat gtagcaagaa ttacaaaaat
    24781 aaaatatcag cagggcgttg gcagtagcct tgaagtgatg gatgcagaaa cagctttgaa
    24841 agtagcagaa acaaattatt atgctgctat ggtagaagca tttattgcca aagtagattt
    24901 tgaaaaagca acaggaacat taataaaata aaatataaac tctgacataa ttaaatatat
    24961 gaaaaaggta attatagcaa taacagcaat tggatttctt actgcctgtg gtggaggaaa
    25021 cagcagtaaa aaagttgaag aacttgagaa actcagaaag caacaatctg agctgaatga
    25081 aaagataaaa tcgttagaag cagaaattgc tcttacagat tctaccagaa aagacaaaat
    25141 aaaatttgtt gcagccactg atatgagtct gaaaccattt actcattata ttgaagtgca
    25201 ggcaaatgtt gagggtgatg aagatgtaat gttaagtgca aaaactcccg gaaccgttac
    25261 ctctgttaat gtaaaagcag gagatcatgt aaccaaaggt caggtgttag ctacactcga
    25321 tgatcagatt atgcaaaaag caattgcaga agtgcagaat gaactcaatc tgttgacaac
    25381 actgtataac cgtcaaaaaa atctttggga ccaacagata ggtagtgaag tacaatatct
    25441 ggaagcaaaa acaagaaaag aaggtgcaga gaaaaagtta gcatcaattc aggagcagtg
    25501 ggatatgact cgtattaaag cacccttcag cggcacagta gataatgtga tgatcaagac
    25561 aggacagttg gtttcgccac aggttcctgc aataagagta gttaatctgg agatgcttaa
    25621 agtaaaaggc gagttgcccg agacttatat tggaagagta aataaaaacg atcatgtttt
    25681 ggtttatttt cctgacttag gaaaagaaat tacgagtaag gtttattatt ccggtaatgc
    25741 catcagcaac ctgaaccgca catttaatgt tgaagttcgt tttggcaaca aagaaaattt
    25801 tctacgaccc aatatgctgg cagttttaaa aattgccgat tacaataaag ataaagctat
    25861 gtcacttcct gtaaatgttg tgcaaaaagg acttgatggt gagtatgtgt ttgttgttga
    25921 aaattctggt ggaaaaacag ttgcaaagcg taaaactgtg aagagtggaa tgacttacaa
    25981 tggcaatgtt gagattgttg atggacttaa tgaaggcgat aaagttataa caacaggata
    26041 tcacgatttg gttgaaggcg atatcattaa actataaacc gaaaacattt tatgaaagat
    26101 atatttaaag agttcaagcc ctcttcatgg gcaattgata atcgtacggc tatctacatt
    26161 attaccatca tcattacgtt gacgggaata atggcctaca tcaatattcc taaagaaagt
    26221 tttcctgata tttctttacc caatatttat gtcagtgttg tttatccggg tacttcacca
    26281 aaagacatgg agaacctggt agtgcgtcat attgaaaagg aatgtaaagg aatttccgga
    26341 gtaaagaaaa tcaagagtaa tgcactgcaa gattattgca gtatagtgat agaatttaac
    26401 agcgatgtaa aaattgaaga tgctaaacga aaggtgaaag atgctgttga tcgtgcacgc
    26461 aatgaccttc ctcaagattt gcctaacgag cctgatataa tcgaagtcaa cttctctgac
    26521 ttgcctatta tggcagtaaa cctttccggt gattatgatc tggcaaaatt aaaacgctat
    26581 gctgatgaag caaaagataa aatagaaagc cttaaagaag ttaaggaagt aaaactgata
    26641 ggagagttag agcgtgagat tcagatcaat gttgacatgc ttaagttgca atcatcagga
    26701 ttgacaatga gtgatattga gcgtgccgtt aagtacgaaa acatgacggt accggggggt
    26761 agtgtgctta tggccgatgg cacacgcagg tcattgagtg tgtcaggtga gtttaccaat
    26821 gtagatcaga taaaaaactt ggttgtaaat tctattaatg gtgctcctat ttatctgaag
    26881 gatattgcag aagtggttga ctcctacaaa gagcaggaaa gctatgcacg tttaaatcat
    26941 aaaaatgtaa ttacattaag tataatcaaa cgctccggtg aaaacctgat tgatgcatct
    27001 gataaaattg tttctgttgt caatgaactt aaaaaagaag catggccaaa agatttaaat
    27061 gtagttctta caggtgatca gagtgaacag acacgtactc aattacacga tttgataaac
    27121 actatcatca ttggattcat tttggtatta gtggtgctga tgttttttat gggaaccagt
    27181 aatgcattct ttgttgccat gtctgtacca ttgtccatgt gtgttgcatt tttagtatta
    27241 cctgttattg gttttaccat taacttcatt gtcttgtttt cctttcttct tgcattagga
    27301 attgttgttg acgatgccat tgttgttatt gaaaacacgc atcgaatttt cgacaatggt
    27361 aaaacagata tagttagagc tgcaaaaatg gcaacaggcg aagtgttttt acctgtgctt
    27421 tcaggaacac tcaccacact tgcacctttt gtgccactgg cgttttggaa aggtatcatt
    27481 ggtaaattta tgttctttct accaattaca ttaatcataa cattattggc atcattggtt
    27541 gttgcataca ttatcaaccc tgtgtttgcc gttagtttta tgaaaaagca cgaagaaaaa
    27601 aatgatccat ataaaggtct gaaaattact tctgtcattt ttatagctat agcattgttg
    27661 ttttatgtga caggagtata tggtatgggg aattttacaa tttttatttt cctgctgaat
    27721 ttgctccacc gtttttggtt acataaagtt attatacgct ttcaaacaaa aacatggcct
    27781 gcagttcagg agttatataa acgcatgctg actaaagtcc ttcatcgtcc acgtactatg
    27841 atggcaggaa ctgttgggtt attgttttta tcttttattg caattggact tcgaaacggt
    27901 ggtgtaaact ttttccctat tgcagatcca aactttgttt atgtttttac cacattacct
    27961 gttggaaccg atcaggcata taccgactct gttacaaaaa ttcttgaaag taaagtttat
    28021 aaagtgttag gagaaaagaa tgatgttgta aaatcagtta ttgccagtgt tggtgtaaac
    28081 gtttctgatc catcagacga agatcagggt tactatgcca acaagagcaa aatagaagtt
    28141 gcatttgttg agtacagtaa aagaaaaggt gtttcaacaa gtaattattt agagaaaatc
    28201 agagaagcag taaaaaatac tgttcccgga gccgaagtta ctgttgcaca agagcagggt
    28261 ggtccacctg taggcaaacc aatatctatt gaagtaagtg gtgatgatct tcaggcaatc
    28321 actacaactt ctaaaagcct taagcgatat cttgattcct tacagattga gggtgttgaa
    28381 gaattaaaat ctgatttaca ggatagtaaa ccacaaatag tgtttgatat caatcgcgaa
    28441 cacatgaacc gtcttggaat tatgacaggc acagtaggaa atgaaatcaa catggctgtg
    28501 cttggtcgcg aggcatcaaa gtatcgcgat ttaaatgatg agtataaaat tatgctccga
    28561 tataaattag atcagcgtta tgatgttagt ctgcttaaca atttaaaaat attgtatcgt
    28621 gatatgtcaa tgggtggatt ggtacgtaat gttccattgg catcttttgc atcagtaaaa
    28681 tacgataaca catatggtgt tataaagaga aagaatcaga agagggtagt aactatttcc
    28741 tcaaacttac ttacagacta tcaaagtcgc gaagcagggg ttgttggtaa tgtgatggct
    28801 gcagtaaaac aatttccaaa aacagaagga gtagaaatta agtttgcagg ctcgcaggaa
    28861 gaacaggcag aaaccatgtc gtttctggga acggcattgt tgatttcttt aggattgata
    28921 tttatgatat tggtaattca atttaactca ataggtaagc ccataattat cttgtcagaa
    28981 attgtattta gtatcattgg tgtattgctt gggtttgccc ttttcaaaat gggattctct
    29041 attgtgatga caggtattgg tgttgtagca cttgccggta ttgtggtgag aaatggtatt
    29101 ctgttggtag agttcacaga gttacttcgt gaacaaggcg ttccaacatt cgaagcaata
    29161 gttgaagcag gtcgtacgag aatgactccg gtaattctta ctgccactgc aacatcatta
    29221 ggattgattc cgcttgcagt aggtttgaat attgatttcg taacactgtt tacagaattt
    29281 aatcctcaca ttttctttgg tggcgatagc acagctttct ggggaccact gtcttggaca
    29341 atgatattcg gattgatgtt tgcaacattc cttacactgt ttttagttcc ggtaatgtat
    29401 ctgatgtcag aaaatctgaa agcaaaaatc tacaaaagac caaagccgat aatgaaaaaa
    29461 ccggacgatg agttaattgc ctgatagaaa ttttaaatgc taattttaga tgaaggctgt
    29521 ctcagaacta aatttgagac agccttttga tttctgttag gtattcttcg actgtaatat
    29581 aaaattctac ccaaactatt atgggctttt aggttcttag tagcaagaaa tgaagcaata
    29641 gaacaaaaat gctttaaaac aaagcagaac ttgttacctc aaaaacctga aataaaaaac
    29701 tacttaataa aagcccgcca gatatcgttt ccgattacaa agaccattaa tgataggagt
    29761 aaaaccattc caacaacctg cgttttttcc ataaatgcat cactgaattt cttgcgtgtg
    29821 acagattcaa tgagcagaaa taaaacatga ccgccatcaa gtgcaggaat aggaagtata
    29881 ttcataaaag caagaatcat agagagcaag cctgtaagta cccaaaagtg tggccaatcc
    29941 caagtgccac cataaatttt tgctatacca ataggtccct gaactgactc ctgaaatttt
    30001 tcttcacctt taaagagttt cttaaaccct ttagcattgc tcactaatgt ttcaaatgca
    30061 tctgatgtac caaatgccaa tgctgatgat aatgtgtaag gtgttttaat atattcctca
    30121 ggtattacca catcaccatt ataacgcaca ccaatagttc cttttgaact tatgtttacc
    30181 gtaatgtcca ttggtttacc attacgaatc attgaaagca atgcagtttc acctttttta
    30241 tcagcaaaat aacgctgcat aaaatcttca ccggtaattt tttgcttatc aacttctgta
    30301 atgatgtcac cgttttctac tccagctttg aatgcatttt cgccaacaac aacttcttta
    30361 acaacatagg tcagatgtcc tgcgccaaga aaaagtgaac gggcattggg tcctgttttt
    30421 ttataaaaat cgtcaggcac ttcaattggt atggtttcac catttcggat aacagttaat
    30481 gttgcaccaa acaatacacg tgttgaaagc aagtcatcaa aacgatcgaa tgattttcca
    30541 tcaatagcaa caactttatc tcctgtacgc aagccaattt cttcccccag tttataagca
    30601 acaataccat gtgtaacaga atcatttttc agatagctgc ctttgtagtg caataaactc
    30661 attgaaaaaa tagcaatacc aagaatgaca ttcattacta cccctgcaac cattacaatt
    30721 aatctttgcc atgctggttt acttctgaat tcccatggtt gtgcaggttg tgctaattga
    30781 tctttatcca tactttcatc aaccatccct gcaattttta catagccacc aaaaggaagc
    30841 catcctatac catactcaca atcacctttt ttaaagctga ataattttat tccccatgca
    30901 tcaaaaaaca ggtagaactt ttcgaccttg ataccaaaca ttcttgcagc aagaaaatgc
    30961 cccaattcat gcagggttac taatattgaa aggcccagta tcaactgagc tgccataatc
    31021 aatccactca tcagatttta aaaattttat gcgaagttaa ttttttttag tcaatagtaa
    31081 ttctgtttgc aatcgtgttt ccttatccgt tgcaatcagg tcttcaagcg atggtttttc
    31141 aatgtaagtt atcttcgaca tgagttgttc tgcaataccg ggaatctgaa agaaactaat
    31201 tttctctttg agaaacgcag caactgcaac ttcgtttgcc gcattaagaa tacaaggcat
    31261 gtttccatct tttttcatag cttcttcagc caaaagcaag gcagaaaaaa cttcggtatc
    31321 aggtttctcg aaagtaagtt ggggatagtc aagaaagttg aatctcttga aatcggtctt
    31381 taaacgatgc ggaaaactta gtgcatacag gataggtagt ttcatgtcag gcaaacccat
    31441 ttgtgctttc atgctgccat cagtaaattg tactacagaa tgaatgattg actgtggatg
    31501 cacaacaaca tgaatttgat gtggctgcaa tccaaagagc catttggctt caatcatttc
    31561 aaggccttta ttcatcattg tagcagagtc aacagtgatt tttgcaccca tgctccagtt
    31621 gggatgtttc agtgcctgag ccaaagttac ttgctctaat tctttcttgc tttttcctct
    31681 gaatggcccg cccgatgctg ttaaataaat cttttctatc gggttatgaa actctcctgc
    31741 aagacattga aaaatggctg aatgttcgga gtcaaccggg taaatgcgca caccgttatc
    31801 atctgcaagg gctgttatta ttttacctgc caccacaagt gtttctttat tggcaagagc
    31861 aatagtcttt ttacttttta ttgctgcaat tgttggcaat agtccggcat agcctaccat
    31921 tgctgtaaga acaatgtcta tggagtcgga ctgtacggct tgctcaacag aatcaatgcc
    31981 tgcaaaaact ttaacaggaa tgtcagcaat attttcttta aatattttgt acttctcttt
    32041 atcggttaca acagcagtgg cgggagtaaa ttgccgaacc tgcttttcga gcagttctat
    32101 attaccgttt gcagttaata actcaacttc aaaaacatca gcatgtgcag caatcacttc
    32161 aagtgcctgc ctgccaatac taccggttga accaagtata gcaattcgtt ttttcaaagc
    32221 tgattgacta tgagcggaca atattagtta aatcctgttg atgtatgaat taatcagcag
    32281 ggatattgtt agctgaaatg ttatgagaga agtggattaa aacaaatgtt attcaacagt
    32341 aacacttttt gccagattgc gaggttgatc aacattacaa ccacgcataa ctgcaatatg
    32401 atagcttaac aactgcaagg gaattacact tacgagtggt acaagaatat cgtcagtttc
    32461 cggaatttca atagtataat ctgccatttt cctgacatca acatcgcctt ctgtaacaat
    32521 agcaattatt tttcctttac gtgcttttac ttcctgaata tttgaaacta ctttttcgta
    32581 tgaagaattt ttagtggcaa taactacaac aggcatttct tcgtcaatta aggcaatagg
    32641 cccgtgtttc atttctgcag caggataacc ttctgcatgt atatatgaaa tctctttaag
    32701 ctttaaagca ccttctaagg caacaggaaa tccataacca cgacctaagt aaaggaagtt
    32761 tcgggattct ttaaacaatg cagatatttc tttaacctta tcatttgatc taagtgcaat
    32821 ttcaactttt gaaggaatag tttcaagttc gttaagtaat gctctaaaac gtgattgtgt
    32881 aatggtgcct cttttatttg caatctgaag cgccattaag gttagtactg ttacctgtgc
    32941 tgtaaatgct ttggtagaag ccacaccaat ttcaggtccg gcatgtgtgt aggaacctgc
    33001 atgtgttgca cgtggaatag atgaccccac aacgttgcaa acaccaaaaa ttgttgcacc
    33061 acgttcttta gccatttcaa tggctgcaag tgtgtcggca gtttcgccac tttgcgaaat
    33121 agctatcact acatcatctt cagagatgat aggatttcta taacgaaatt cagaagcata
    33181 ttcaacctca acaggaatac gtgccaggtc ttcaataaga tattctgcca ccaatgctgc
    33241 atgccaggat gttccgcagg caactatgat tattcttttt gcattgatca atttgttttc
    33301 aaattcgctg ataccaccta atcttaatct gcctttttct gcatcaatac gtccgcgcat
    33361 actgtcccaa atagaacgag gttgctcata gatttctttc atcatgaaat gatcgtatcc
    33421 gcctttttca atggcttcaa gctttaactc taattcctga acaaaaggaa ttttggtttc
    33481 gttgtcaata gtttttacag tcagtttacc attatcaata gttgcaactt caccatcgtt
    33541 gaggtaggca acgtttttag tgtattctac aataggagtt gcatccgagg caataaaaaa
    33601 ttcgtcttta ccaataccca caacaatagg gctgcctttg cgggcagcaa tcattttgtt
    33661 aggctcattc tttgataaaa caacaatggc ataagcgcca ataacttcgc ctaaagcaag
    33721 acgtacagcc tcctcagtag ctacatgggt tttatgttta atctcttcaa taagatgtat
    33781 caaaacctct gtgtctgtat cacttttaaa agtatgacct gaatttatca gctcttgttt
    33841 gatggctgca tagttttcaa tgataccatt atgaattatg gctaaatctt ttgattcaga
    33901 ataatgagga tgtgcatttc tgtcgttagg ttcaccatgt gttgcccaac gtgtatgccc
    33961 catgcccact tgtcctacta aatttttatc agatacatag gcggccagat cagcaacctt
    34021 acctgctttt ttgtaaacac tcagttcacc attcaataat gccacaccgg cactgtcata
    34081 gccgcgatac tccagacgct gaagtccttt aattactatc ggataagcct gacgatttcc
    34141 tatataggca acgattccac acatgatatt ttatggattt aattttgtat aagtaatttg
    34201 taatttcata cgtaacgttg atgaaggatt acctcctgtc aaaactacac gattgggtgt
    34261 ttgaaaggct accggcaccc ttattcttaa gtacattccg tagtctatac gtttttcgta
    34321 tagaatctgt tgcaaaaact gtgcaatatt aaaacggtac tgattatttg ccagtccacc
    34381 gccaaaaagt acagatgatc gtgtcaaatc aggtaaggtt tgtatttgcc ccacggaatc
    34441 tatggcataa agactcaatg catcaggggc aggaagattg gttgttgttc ccggatcaac
    34501 attgatgatt aattctgctt tattaattgc tattggcccc atttctttca gactgttgag
    34561 ataaggaatg gtgatttttg ttttaattcc gcccattgtc tgaacataag ctttatctgt
    34621 cagaggattg gtgaaattaa atgctgaata atcatgtgta aacctgctga aacgggcaat
    34681 gctgccatcg gaactgtaca tgtaaaatgg taatgagtca atgcctgttg ttgccggatc
    34741 tgtaaaatac attatcattg acgaaacagt tgtttttgca tcgaaattga taatgctccc
    34801 gaatgatgac gctgcaggtt ttatacaaaa gcctttgacg gtttctttaa attttgatgg
    34861 atcacttaaa tcggagttaa aaattttctg acctaaaaca tcatcaagtc gcagacgtag
    34921 tcgtggggca ctttttgttg tgtcagtatt tacaatactg tcttttacat cttcaacctt
    34981 aaatgttatt tcgccaactt ttgtactgta attaaggtta ctgaaagcat aataacttgc
    35041 tgtgtcatac atggcatcag tgagttcata aacctcaaaa gtctgcaagg cagaagtgtc
    35101 gccatagtta gaaatatatg ccagattcag aaataatgag tcgtatgttt tgtttgtatt
    35161 ggaaaaaact gctgatggtt taacttgtgt aaataactca gcacttgttt caccaaaaac
    35221 agggtcaaca tatttcccaa tcatcaactg atgattggta gcctgaagtt cataaagcag
    35281 gtgtgcatcg gtacgcaggc tgtcttcctg tttgtatgat gtctgaagtg ttaacgtgtc
    35341 gaaaaattca acattcatag catcttctcc gggcaaaagg ttcaaaccaa cagaatcggg
    35401 cttgttgcat gagatactta gcagcacgac acatgccatc cacaaatgct taaattgttg
    35461 catataaata aggtgtggtt tttttaaata attaatgttg aacaatatcc gcttaggctt
    35521 ctattgagat ttcttccatg atttcatcat agaaattatt gtatgcctca acgtaatttt
    35581 caggcgattg gtatggtaaa attaatttgc ctgatttttt aatatgatta tctatcttgg
    35641 tattgagttt ttcagcacct ttaatgatag catctgactt gtttatggca gcaattgtta
    35701 tattctcgaa gtttggactt tttatgtctt ttacatcatt agtagaaatc ccttccatca
    35761 tgagcttttt tgagtaactg tcatgaaaag attcactaaa ttcatcatca taaacagaat
    35821 aaactactct tgcatttcgg aaaatagggt cttcgcgata ggctgttttt acaaacagcg
    35881 gaatcagact agacatccag ccgtggcagt gaataatatc gggtgcccaa cctagttttt
    35941 taactgtttc aagaacacca cgacagaaga aaatggttcg gtcgtcatta tcctgaaaaa
    36001 atttgttatc tgcatcatga gtggtatgtt ttctttgaaa atactcctca ttatcaataa
    36061 aataaacctg catacgggct gcctgaatac tggcaacctt aataattaac ggatgatcgc
    36121 tttcatcaat aatcaggttc atcccggaaa gtcggattac ctcatgtaat tgatttctac
    36181 gctcattaac aacaccaaaa cgcggcataa aggttctgat ttctttacct ttctcctgtg
    36241 tgccttgtgg cagatgtctt gcaatgtttc ctattgttga actttcgaga taagggaaaa
    36301 tttcctgcga tacgaacagg accttgggct ttttattcat gcaatctact tctcttaaaa
    36361 tttacgtatg caaaaatata taaaaattcc ctttgaaccg gcaaaatttt agcgatattt
    36421 tgttaaagtc attgtctgaa aggtagaatt actggttttt aggttaaaaa tcaacacttc
    36481 tgcccttagc cgaagagtgc ttgatatact tttgatggat aagatatgaa ctcgtattgg
    36541 aaataaaata gcaattcaac tatcatgaaa aagcaaatca ttcaaagtgc cttaaggcta
    36601 ttaaaactaa accctttgtt tttatttata cttgtttgtt taccaatagg ctttatattt
    36661 tattttaccc ctgctgaaga ttttcttgtt tttaaaaaac tgttttccag cctaggctgg
    36721 ataatttatt ttttctggat ttttgctata ggacaccgag cgatagaaaa ggtggtttca
    36781 caaaatattg catggggtgg attccggtat tttattccgg gatatgttgt gagtgccata
    36841 ctttttatag tgatgttatt tacaactaca aaagaaaaaa tccaaatcgg acttttgcag
    36901 gttgagtaca ttgcaccatt agttcttaca ataagtgcgg cagtaatgtt tttaatagta
    36961 attttaatag catcaaaggc aatagtaagt gcagaaacgg gaagcaatgc aaacttgggc
    37021 agcttcctgc ctactgcctt actgctattt ttttttccaa ttgggttgtg gtttattcaa
    37081 ccacggatac agcgattgta gcaaccggta gcaatttttg ttaggggaca tttttttaat
    37141 gtaatgttca agttttaaaa aattggcaag gaaatttgtt gcataaaatc cggaaaatcc
    37201 tatttttact ccggcttaac agtaaagaac aatcactatc taaattaaca tcaccttgac
    37261 agcactcatc atcttaattg ttgtattggc aatagttgcc ttctacttta tcagtgttta
    37321 caatacactt gttactctta aaaacaaagt tcaggaagca tggagcagca ttgatgtcat
    37381 gctaaagaaa cgaaatgacc ttataccaaa tctgatagaa acagtgaaag gttatgctgc
    37441 acatgagcgc aatacacttg acaatgtgac gcaggcaaga gcacaatctg taaacgcaaa
    37501 aacggttggc gaacaaaaag ccgcagaaga gaatatgaac aaagccatga tgaacctgtt
    37561 tgctgtggcc gagcagtacc ccgacctaaa ggccaatacc aattttataa acctgcagca
    37621 agaattatcg gctattgaag gtgacattga aaaatcaaga cgctactata acggaacagc
    37681 acgtgacaac aacattgcaa tagaaagttt tcccagtaat attgtggcca acatgtttca
    37741 gtttaataaa gtagagttct ttgaactgag taatgaaagt gaacgtgaag ttcctaaagt
    37801 aaagttctga catcatcctt atttataatt atcttatgag aacaaaccgc agctatctct
    37861 taggaattgt aagtttaata tttatactta tcaactccaa ctttgccagc ggacaggagg
    37921 aaagaatatt gagttataac tccgatattc aacttaatcc tgaaaatgag ttgatggtga
    37981 cagaaacaat caaggttgtt gcaaaaggaa ttgaaatcag aagaggtatt ttcagagtct
    38041 tacctgaaaa caaatcaaaa tcgaatccgc taaaagatgt ttcttatgac attgtttctg
    38101 taaaaagaga tggcaaggaa gagccttatc accttaaaaa cgaaaatggc aatacagtag
    38161 tttatattgg tgaaaaaaac gagtacctcg atccgggcat ttatgtatat gagttgcaat
    38221 acaagacttc aaatcatgtt ggatttttta atgattatga cgaactttat tggaatgtca
    38281 ccggcaacga ctggagtttt catatagata gtgttagtgc taccatacac ctgccggcat
    38341 ctgcaaaaat tttacaacaa tcttgttata ccggctatgc cggttcaaca aacaccaact
    38401 gtacctctca aaaattgtct gatacagaaa tcaactggtc tgcagtaggt ttaaattcaa
    38461 acgaagggtt gaccgtagct gttggtttcc ccaaaaacat tattgctcca ccaccaccac
    38521 cgggcttcgg acaaaagtat gccttgcttt tgtttgcaat aattgttgta tttctcttgc
    38581 tcatttatta cttttttacg tggagtaagt atggtaaaga tcctgaagca cctgcggtct
    38641 atcccatctt tgaatctcca gatgctatct caccggcaat actcggatat gtggatgctg
    38701 aatatttcaa gaatgatttt gttactgcat ccattgtcaa cttagctgta aaagggtatc
    38761 ttaaaattaa tgaaaaagaa aacaaagtat tattaggttt atttaaagac accaattata
    38821 gtttaaccaa actgaaaaat gcagactcct cccttccaat ggaagaagca atgcttttga
    38881 acaagctgtt tagctcttcc aacgaattaa gtttaacagg cacctacgac cccaacataa
    38941 agaatgctgt tactgcatat acggattcaa ttaaaagcca gtacagtaat tttatcaata
    39001 agggaaataa ttttaaatta atagttgcac cttctgtgat gattgcactg gcaataattt
    39061 taatttccat ttttgatgca atgatgattg acagattgtt gccattgttt gttattgcca
    39121 tcttcggaac ggtttcttta tttattattt cattcatctt cggattcatg ggtaaacgtt
    39181 tttataagac catgtggttg attgcaatat tggagatggg acttttactt tatctttcat
    39241 accacacact ttatatagca gaagatcatc ttgaaaacaa cctcaccgca cttgctattg
    39301 tacttctgtt tggtgcagcc tccattatgt tttacactta cctaatacgt aaacccagtg
    39361 ctgaaaaatt aaaattacag tcgcagataa agggatttgg catgtacctg agtgcagcag
    39421 aagaagagca gttgaagttt tttaattcac ctaaaatgac tccggaaata tttgaaaaat
    39481 ttttgccgtt tgccatggtt ttaggagcag ataaaatttg gggcgaaaag tttgataaca
    39541 tgttgaagag tgcatccatt cagaatacta catacaacag ttcatggtat tcaggaagtc
    39601 atttgtcacc ggcattattt tcaagctcgc tgaattcttc tttttctcat tctattcagt
    39661 ctgcatcaac tcctccatct tcatcaggta gcggaggtgg cggatcttcc ggaggcggag
    39721 gcggaggtgg aggcggaggt ggctggtaat gctacttgtt ttataaaatt caggttactg
    39781 catcaggttt tgtgcaagat ggcgagtaaa ttttttctta tagcataagt cattataaat
    39841 tgcttgttca ggtaggtagt atatgccaac gttttcattt tttagtttta acagcgtctc
    39901 ttcatcggca taatgcaaaa gcattatttg tgggttcagt tgtccgatac tcaaaatgga
    39961 aatcatatta acccaatagc cacaaatcac tgcattgcga ccttgcagaa ggtgcgtttt
    40021 ttcaataacg cttacagcat attttatccg ctgtgttctt ttggcataat catcagaaac
    40081 atttccacta aaagggtcaa aacatattct aaggtttccg acttttctgg tcagcgcata
    40141 ttttccgggc aatgccgagc ggttgggttc ggctagattg atacctgcaa aaaaacaact
    40201 taacaccaat gatgtggtaa ccagcatcgg ccaaagttta tcctgatgtg caatgagtat
    40261 tactaatatt acaaaaggaa taacaggaat aagataggct gatttatgtg gctcataaag
    40321 aaacaacagt gtataaataa taatagcaga gaaagcacca aacaannnnn nnnnnnnnnn
    40381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40441 nnnnnnnnnn nnnnnnnnnn nnnnnattgc agtgatgcgg catcctgtag ctaaagcaag
    40501 tgtgagtgct gctaaaaata tttttcttct tcctgcaaaa tagagtgctg ctaacaaaaa
    40561 acacagcgac cagttatagt ccattgcatt ggtactttga atataaaaaa caggagtaaa
    40621 agcaagcatt aatgctccgg cagtagcatt gagtttgtat tttttgcaga ttaaataaaa
    40681 cagcaacaca gcaacggtac ttaagagcgc tgtacatagg ttgaaagcaa aagaatgttt
    40741 tacaggtaaa aagtaatacg cccattcggg tacaggatgt ccgggcaaac gcgaatactg
    40801 ataactatgt gttgaatcca tagcaagtgc atttacggca tagccccatg catcttcttc
    40861 ggcaccatag cctgcatcaa gaaaaaacca acggctggca aatactaata atcccaaaag
    40921 gataagccgc atagtttgat tcatggacgc aaagtaacat gttttgacca tttttgtatg
    40981 attttgattc tattttaaca acacatttat atctttacgt aattgcaaat aattgcagca
    41041 ccgtcatggc aatgttatta gagttaacaa ataagggtat ttattgtccg caggcaaatg
    41101 tctatattga cccgcatcgc agagtggaaa atgccatcat cactcatgca cacagcgacc
    41161 atgcacgccc tggaagcagt cactatcttg cacataaaga ttcggaagct atgctgcgta
    41221 tgcgattggg cgaacaaatt agtttgcgca cggttgctta tggtgaaaca tttactatta
    41281 acggagtaaa gttttctttg catcctgcag gtcatattat cggcagtgca cagataagag
    41341 tagaatttaa agacgaagtg tgggtggcag caggcgacta caagttgcat gatgatggtt
    41401 tctgccccgt gtttgaacat gtaaagtgca atgtgtttat tacagagtca acctttggct
    41461 tacccattta caaatggcag ccacaacaac agacaatgca ggatattact gcatggataa
    41521 ataccaataa tcaacaacaa actacttctg tgctgatggg ttattcgctt ggcaagatgc
    41581 aaaggctgat aaagcagtta aaccattttg attttggtat ctatggtaca tctgatgttg
    41641 tggctgtcaa cgaaagatta cgtgaagccg gttgtgattt gccttatgtt aaaccgctga
    41701 aatcaaactt tgtgaaagaa aatctgaaag gtgcattaat atttgcatca ccatcaggag
    41761 cacgttcggc attgtttaag aaacttgaaa actatagtgt tggctattgc agtggttgga
    41821 tgttgcatgg caaatcaaaa tatttcagcc atgttgaccg tggttttgca ctcagcgacc
    41881 atgccgactg ggatgagttg aatacagcag ttaaagaaag tggtgcagag aaaatatatg
    41941 taactcatgg tagtactaca gcattttcaa aatggctttg tgacaaaggt tatgatgcaa
    42001 aagaaattga attcatgaag ggggtagggt agtggagagg gtgactcgtc caatttcacg
    42061 acaagtcagt atataaaaac tacctaatca tcacaccgcg cttttctagc aatggtatct
    42121 gtgtaaaaaa ctcctcattt ataactcccg gaagaaaaac atatttacga tcatacatgg
    42181 tgttgttaag ataaaagctt acccagtatt cattgttcag gttaagaaca ttatctgtaa
    42241 taggttcaat ctttacccac gactgcggag gaatattttc tataaaatgt ctcagggttg
    42301 acgtttttct ttcttcacct tccatgatgc catagccttt gcttgtaatt aatacacctt
    42361 gcaacacttc atttttcaag ttaatgatat aggcattcca aacatcagca ccgtttgatt
    42421 gttcttttac aatggcaacg gctacgtttt caacttttaa ttgaggaata tcttttagca
    42481 tattatatta aacgaataat gaagttagaa gttaccttat agcatgttat tcatacattt
    42541 ttatgacttg ttcggcataa aatttatagt cattaggctt tccgtccggg ttgttgctgc
    42601 gtttatgtcc caatcgcaca ataatcatct gcatatcggg aataacaata acatactgcc
    42661 ctaatatgcc acgcatgtag tacacaggat gattgctgat gttagatagc caccattgat
    42721 agccgtaatg ctgagcaggt ttattatctt ccttatccgg caaattattt ggtgtggtac
    42781 aaagtgctat aaaggcagag tcaataattt gttcgttatt ccatcgtcca tgttgcaaaa
    42841 caagtttacc gaaacgtgca aagtcgcgtg cattgctgta aaagcaacag taagcttttt
    42901 ctattccatc tttttgatca agactccatt gtgcattgtg ttctgcaccc aatggtttcc
    42961 aaagtttttc tgatgcatat tcagccagac tttttcctgt tgctttattc acgatgatag
    43021 ctaacaattc tgtatcgcag cttttatatt cccagaattt cccaggtgct tctgcaacac
    43081 ctaatgaggt aacctgtttg taaagttgtg ttccataata ggcttcagta acctgcgatg
    43141 tcagcgaggc atatccttca ttccaatcaa gacctgaact catcatcatc aaatgacgaa
    43201 ttgtaatttt tgttttttca ccttctttaa actcaggaat aaaatctgca actggctggt
    43261 caaggctttt aattttacct tcgtcaatag cttttccaat cagtaatgct acaatacttt
    43321 ttgccataga aaaggaattg gaaagtgatt cctgcccgta gttttcccaa tatttttcaa
    43381 aacaaataga gtcgtttttt aaaactaaaa atgcaacaga tttgttagct tctaataatg
    43441 cagtgagtgt gtcgctcaga tttttcttat tatagtctgt agaaacattc catggcattc
    43501 ctgttccgtt ttttacttct ctgttgtaaa acaaattcaa atcgtcaata tctacaaaat
    43561 tgtaaacaag agctttgtag taatacaatt ttcccgtagc tgcaagtgct attgttgtta
    43621 tcagtaaaat aaacagcagg tatttgatta tctttttcat gttttttctt ttgctattag
    43681 tttttctttt tccacagttt taattgtggc attaaaaaca tcactgaagt gttttaataa
    43741 aacagatttc acttcatcaa tattcaaatt atttccaagt tcttttgcca tggatgtaac
    43801 agctttgtcg ctgatgccgc agggaataat gtggttaaaa tattccaact gtgtatttac
    43861 attgagcgcc catccatgca tggttatcca tctgctgcaa cgtacaccaa aggcacaaat
    43921 cttgcgcgca cttggtttgc ccgcatcaat ccacacaccg gttagttttt ctattctgct
    43981 gccttcaatg ccatattctt ttaaagttcg gataattact tcttcgagaa aacgcatgta
    44041 gcgatgaatg tcattaaaaa acaggtcaag attcagaata ggataaccaa caatctgtcc
    44101 aggaccatga taggtaatat caccaccacg atttatttta taataatctg cattggcatt
    44161 tttaagttgt tgttcattag aaagcaaatg ttcgggcttt ccgctttttc ctaaggtgaa
    44221 aacgtgggga tgctcgcaaa ataataagta atgtggcgtt ggtgcatgat cacccaaacc
    44281 ctgattacgt ccttctaatt tcagttgcat aatctgttga agaagctgct cctgaaaatc
    44341 ccaggtttgt ttgtaatctg atgaacctaa atcagcaaaa aatatttcct gcactttttt
    44401 tcttataatt tacaaagata attgaatagg agaaacgatt tttcaacgat aaagtttact
    44461 cactgcaagg caatgataaa tgatttcaag agttgaaaca cattgttgta ctttgttaac
    44521 ggcaaagttt ctgacttatc cattacatca tgataccatg taacaccacc gagtgtatag
    44581 ataaaaaatg caggcacacc cttttcactg aagtaatagt gatcactgtt ttttgcttta
    44641 cctcttttgc caacattgct aacatatttt gcagtgtcgt ttatagactg taaaattgaa
    44701 aaatctcttt caaattcagt tgcattaaca accataatgc cttcatcgcc tgtgccaagc
    44761 aaatcgagat ttatcagaaa acgaattttt gataatggca gtagcggatg ttctgtataa
    44821 tactttgaac cctgcaggcc ggcttcttct ccagcaaaag caataaataa aatggaatag
    44881 ggttgtctgt tttcttgttt tgtaaaatat tcggctaaag aaagtaacat ggcaacacca
    44941 cttgcattgt cattggcacc gggaaagaat gttttatttc ccatgccacc caaatgatcg
    45001 taatgtgccg ttatcacaat taaagagtca ggaattttat ttcctgtaat tactccaatt
    45061 acatttcttg agttatattg tgcattaaat tctgcatcgg catgaagtgt gatacttttt
    45121 gtttgacgac tgaaaaggct atcatatact tcaataacag gaatattaaa cactgacgtg
    45181 ccaacactcc atgtcaattt atgcggttca agaatgacca atacgccttt ttttcgctta
    45241 aaaagcaagg atgtaatttc tttttgcagc ttactgaaaa cctgacgtgt tatgacatag
    45301 caactgtcga aatcattatt aagacttgaa atgatttctg cagaatctgc ccatgctacg
    45361 ggccatgttc cattggcaga tggtgattga tgatgtatta aaaactcttt tccgggaatc
    45421 caggtttttg tgtcagtagt cagacttaca tttttcggaa aacggtttac cggatgtgta
    45481 aaatgctgaa accatttatc atcaacaggc tttaatccaa gtttgcagaa tcttttttta
    45541 ataaaatgtg cagctttctt atcaccatgt ttcaggtaac cacgaccaaa atatttttct
    45601 gaagataatt cagtaataac ttttcgtgca tcatcttgct gtgcaaaaac aagatgtgta
    45661 cacagcaata cataaataaa aatcaacgat gcaaaaaaag atattcgcag cattacatga
    45721 atcgcctttg cagcaaatct acaaactgtt tgcaggcatc actattctta tctatctgat
    45781 ttcctgaagt tatttcagag tcgaagtaca tgtaggcttc ggcaaaattt gctgaggagt
    45841 tcaacttatc aattgctgat tgatatctta ttgaatcatc attaaacaac tgactgatga
    45901 ataagaattt ttcattgatg ccaatggctt taaccaaatc agcaacaggc gacagtttta
    45961 atttatctgc tatggcaaaa gccggctgtg ttatacctat tttatcgtga atggtttccg
    46021 tctcgtgaaa catagttgcc actgtttgat ccacttcttt aaaaacttca gctcttacag
    46081 tttgtactgg tggaataaca ggttcagcgt ttttttcaga ttttacttct tcaacaggtt
    46141 ttgcttttac ttcttcaatt attaaagctt ctacttttaa ttcagttttc ggagtttcaa
    46201 caacagttcg ttgttcgggt tcagattttt caatgacagt ctgtactatt ggctgacgta
    46261 ctgtttctgt aacaacctcc tgattcggtt gctcataaga ggaaacagat tcaaccaatt
    46321 gaagatgatt gattttatca tgtagtttca tcagtttgct tttcagcatg tccagttcat
    46381 cattttcagg aatacggttg tctaatgaaa aaacgcccca ttgcctgtag atctgttcaa
    46441 ttgcaacttg tatttcgctg gtgatagatt gctttgtcat gaaattgtgt ttttatgcta
    46501 acgctaaata aattaaatca tttttgattt ggaaaattta tggataatct cagaaaattt
    46561 tcatgagcaa aattaattgt catacttctg tcaacattgt agattgactg atttttatca
    46621 acataacttt tccatgctgc agggtgctac ttttactttt gcagctgtga aaaaacccat
    46681 actcctattc acattttttc ttttgtctgt aacgctcttt gctgccgatc aggtattatt
    46741 tatcaaagat aaatccaatg gtaagccggt aaacgaggct gttgtgattt ggataatgcc
    46801 ttccggaaag tcacagatat tactgcccga caacaaagga cgtgttaaac ttcccgaaac
    46861 aaaagaatca gggtttttaa ttgaagtgcg ttgtcttggc tatcagagtc aaaaaattga
    46921 aaaagaaact tccggtgaca ctatttggct aatcagaaat ggagtcaacc tcaatgaagt
    46981 tgctgttact gcttcttatg ctcctgcacc tgccatgcaa tctgcctatg caataaaagt
    47041 aattggtaaa gaaacgtttg acaatatggg tgctgtgaat gttgccgatg tacttagcaa
    47101 acaactcaat atgagcacag gttatgatca ggctttcggc agtacattaa ccatgatggg
    47161 aatggacatg aagaatatta aagtgctgat tgacggtgta cctgtgattg gaagaatgga
    47221 tggaaatatt gacctcaacc aacttaatct tgcagctgtt gaaagaatag aagtcgttga
    47281 aggacctatg agcactgcct atggcaccga tgcaattgca ggtgctatta atttgattac
    47341 aaaaccacaa acaatcagga agactactgc gggcgcctca gcttattacg aaaatattgg
    47401 aacctacaac actgacctga atgtctctgt aaactttaag catgcaggac taaaagtaac
    47461 tgccggaaga aatttttttg aaggatggaa cctgattgat aaccgaagag tttttgaatg
    47521 gaaacccaag acacaatatt tcggcacagc attgctgcaa tatactttca gtaaggttaa
    47581 ggtagcttat cagatgggac tttttgatga gaaaattacg gataaaggtc cagtcactgt
    47641 tacacccttt tctgcttatg cttacgacac ttattttaaa acactgcgca acaatcattc
    47701 agtcagctat gattggtttc tttcaggcaa aaataaaatc aatggtcatg tggctttttc
    47761 aggatatgac agacataaag aagttttttt gaaagatatg gttgctctcg aacagtcaga
    47821 acttacagga caggatttta gtggtgaaac aaaattttac agctggaaca tcagaagtgc
    47881 attcaaccat gttgatgaaa ataaaaactt caattatcag ggaggtatag attttaacat
    47941 tgaaaatgga accggtaagc gaatcagcaa taaggctgcc atgcaggaat atgccgtgtt
    48001 tgcaagtgcc gaatacaaac cattctctaa actacttatc cgtccaggat tacgttatgc
    48061 tttacattca gaatttacca ttccattaat tccatctctt aatctgaaat atgatttaac
    48121 aaacattcag gctattcgtc tttcttacag tcgtggtttt cgcactccgg gaattaaaga
    48181 actttatctt gattttattg atatcaacca caatgttcag ggaaacaaca atattaaacc
    48241 ggagtcgtca gataattttc agttggtata ttccttatta cccgaattca aaggcataaa
    48301 aataaattca gaggtaaaat tgtattacaa caaagtaaaa aacctgataa cattagctac
    48361 ttatagtgat acatcaaacc tatatacata cattaatatt ggacacttta catcacaagg
    48421 cattgaatgg tcaaataaaa ttgatgttgg aagtttttca gtttcagcag gtgtttccta
    48481 tactggaaca tcatccttca acgaatcact tgttaatgat ttatcatggt atctccaggc
    48541 aaatggttct gtgagttatc actataaacc ttttggattg actacaagtg tttattataa
    48601 atacaatgga aaagttcctg tgtatgtttt agacagtaat ggcaacagcc tcaagcaaac
    48661 caacgaagat tatcaactac tggatgcaac tgtttcaaaa aaaatgtgga aggacagaat
    48721 tgaaatagga ggggggctta aaaatatatt taatgttacc gatgtaagac gattcgctac
    48781 aggtgaaatc cacagcggtg gttcatcatc ttcatctcta cttcccggac gattattttt
    48841 cttaaaactt tcagtgaata tttaacatga aaatcaaggt caatattttc tttttattat
    48901 taccaattac agcattgctt tttgcttctt gcgaaaaaga taatgacaag gcaattgtgc
    48961 tgcctcctat tgatactaca ctacaatcca tgcagataaa tattggagtt aattacgata
    49021 ctgttgtatt cgttagcctg acgtccggtg ttgtaaaaag cagtgcagtt aaagattatg
    49081 atttggcttt tgatgcttca ccggaatcaa aacatatata tctcaacaca ggtaagtaca
    49141 tgtttgcatg gcgcacacaa tgtacagata tgatgaatac tgataccaca ggactgaatt
    49201 ggcttactga ttctgataat atgcttggcg acagcacagt ttttggaagt aatacaactt
    49261 caagtggtga tgtaaatcaa caaatcgtaa ttattgacag aggaaaattc agtcatatag
    49321 gtagcgaccg atatcgcaaa attcaaattg tgagtattga cagcaacacc tatacaatcc
    49381 gttacagcaa attgaataat tccgactacc atgagtttgt tgtaacaaag gacaatgcct
    49441 attcgcaggt ttatttttca ttcgacaatg gtggccaaat gaaaaatatt gctccgccaa
    49501 aagatcaatg ggacatcatg tttactcgtt atatccatac ctattgggac gagccattgc
    49561 aattcagata ttattctgta aatggtgtgc tgtctaaccg ttggaacaat ataacgtgtg
    49621 ctattcttaa aaaagattct ttaccggggt atatgtcgtt cgagaatttt aatcataacg
    49681 atatttcttc ttatcctttt tattcaacag gaaacatcat cggctttgaa tggaagtatt
    49741 tcaattttaa cgacaataag tattacataa ctcccgacat atattatatt ctcaaggata
    49801 aaaacgatca gtattataaa ataaagttct atgactttta caatactact ggtgtcagag
    49861 gctatcctgc attttgttat caacgtattt tataaacaac taaatatata tacaaatgaa
    49921 caaattctat tctataatta aactttcagt agcactgttt atgcctctcc ttggcttttc
    49981 acaaatgagt gaaacagtta ccattaatcc cggctacacc aatcagacgt tttacagtat
    50041 ggccaatgca acggtatcaa ctattgacaa taccgattgg gatcttgcat ttcagatttc
    50101 aggatttgaa gcagccattc tcattaattc aaaaaataat gtaaaacttt ttaaagcaaa
    50161 taaagatgct tccgagtggg ccactatgac tgctgcagat actacaggaa tgtattattc
    50221 tgaattgctg aataatgaag cgcacatgtt taatggtgca tttaacacta ctgctgatac
    50281 atcgaacatg tttgatctcg gctggggaga gtatgattta ggctcacacg ctgtaatggg
    50341 tgattctgtt tattttataa aactaaatag taatacagtt aaaaaaatct ggattgaatc
    50401 gcttatcaat gcaacttata attttcgtat tgctgattta gatggtagca atgaaattca
    50461 acgttcatta caaaagttga gctatcctgc aaagaacttc ggctactact caattgcaaa
    50521 cgatcagttc ttggaccgcg aaccaacaaa agacagttgg gatctttgtt tccagcaata
    50581 tgttgatcat tttataaatt acaaagttac aggtgtactc accaacgata gtgtgcaatc
    50641 tgttaaagct tatcctgtaa atgatg
//
LOCUS       DAOQNP010000010        50574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_195_length_50574_cov_8.982620, whole genome shotgun sequence.
ACCESSION   DAOQNP010000010 DAOQNP010000000
VERSION     DAOQNP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 50574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50574
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195_length_50574_cov_8.982620"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKY09_02565"
     CDS             complement(<1..218)
                     /locus_tag="PKY09_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11767.1"
                     /translation="MIKGKWQHSDPLLKMLLFCGIVMTCFGLISVLNIPLVKIFFGKS
                     LSEVMTGFSSGDTTYINAMKFIQIFSQLG"
     gene            273..1259
                     /gene="dusB"
                     /locus_tag="PKY09_02570"
     CDS             273..1259
                     /gene="dusB"
                     /locus_tag="PKY09_02570"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNB11768.1"
                     /translation="MVKIGSVKLGEFPLLLAPMEDVSDPPFRFVCKQHGADLMYTEFI
                     SSEGLIRHAAKSTHKLDIFEYERPIGIQIFGSDVESMKQAAIIAEQAHPDLIDINYGC
                     PVKQVACKGAGAALLQDIPKMVSMTKAIVDAVKLPVTVKTRLGWDDKTKNILEVAERL
                     QDIGIKALTVHARTRSQMYKGSADWSLVGELKNNPRLTIPVFGNGDVDSPQKALEMKN
                     RYGVDGIMIGRASIGYPWIFNQIKEYLAGNCVIAVPGIAERVEVCRTHLIKSVEWKGP
                     IVGILEMRRHYTNYFKGMDHFREFRVKLVTSNSLDELLEILEQIKEKYELAA"
     gene            1256..2068
                     /gene="murI"
                     /locus_tag="PKY09_02575"
     CDS             1256..2068
                     /gene="murI"
                     /locus_tag="PKY09_02575"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNB11769.1"
                     /translation="MIKTQQPIGVFDSGIGGLTVAAAISRLLPHEQLVYFGDTAHVPY
                     GDKSVELIGSYAERITEFLLDEQKCKAIVIACNTASAAAYSLLRDRYKGSVPVINVID
                     PMIEAVIADDDIKSVGIIATKTTIASGIYQEKFSRRKPQLLFHPLATPLLASMIEEGF
                     YNDNISSAVLEEYLSHESFKSIDGLVLACTHYPLIKKEIDHFFKSKVKLFDSAEVVAS
                     KLKKILEAESLVNSERKDSNRFYVSDFTESFEHSTKIFFGKQVKLELMNLWK"
     gene            2081..3004
                     /locus_tag="PKY09_02580"
     CDS             2081..3004
                     /locus_tag="PKY09_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNB11770.1"
                     /translation="MKKFTIAIHGGAGTISPSTMTPQKETAYKQALQAALTAGYKVLE
                     AKGSALEAVTHAVIELENTPLFNAGRGSVFTYDGKHEMDASVMNGKNGLAGAVAGVRN
                     VKNPVLLANAVMEHTDFVFLHGSGAEELARVQQLTFCDDQYFFDDFRYQQWQEVKGSD
                     TTKLDHSNEKKFGTVGAVAKDIEGNLAAATSTGGMTNKRYSRIGDTPVIGAGTYANNS
                     TCAVSCTGHGEYFIRSVVAYDIHCLMEYNGLSLKEACEKVVLDKLVKSGGEGGIIAID
                     QTGVPQLIFNSEGMYRGYYCCNEQPVTEIYR"
     gene            3010..>4652
                     /locus_tag="PKY09_02585"
     CDS             3010..>4652
                     /locus_tag="PKY09_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNB11771.1"
                     /translation="MIRFLLLFMFLGNVCLSQKTTEYYLHNRTPQWHEVIEYYKKLDM
                     QFPQAKLFTEGTTDVGKPLHLFVISADGNFNPVSLHQKRRLIWLINNGIHPGEPDGID
                     ASMIFAKNVLSKINSVAYKNVVICIIPIYNIDGALNRGCCSRVNQEGPLEYGFRGNYQ
                     NLDLNRDFVKCDTKNTESFIRIFQKWNPDLFLDMHVSNGADYPYNITLIATQKDKLTP
                     PLGKYLSEKMLPELYRNMESNSEKMIPYVNTIKDTPDSGLEGFLETPRYASGYTALFN
                     TIGFISETHMLKPYPVRVKATITFMESLRQYAARNAVEILQKRAEANSYLKTIHEFPV
                     QWQLDTTSKDSIRFDGYTAVYSKSKITGLQRLTYDRSNPYSKFIPYQNNFKPQVIIKR
                     PVAYIIPQCWSRVIERLKLNGVKIQTITADTIIEVECYKIGQYKTTSKPYEGHYLHSN
                     VAVEKQIRKVQFYKGDKMIEVNQPANRYIIETLEPQGTDAFFAWGFFDGILQQKEWFS
                     DYVFEDIAEKLIQENPVLLDSLKDAVKSDSTLQNNHYMQ"
     assembly_gap    4653..4752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4753..6245)
                     /locus_tag="PKY09_02590"
     CDS             complement(<4753..6245)
                     /locus_tag="PKY09_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11772.1"
                     /translation="MKKTVFFLSLMFAQVVHAQTLKEAIAMTDNEQYEIAGDMFKSLL
                     QKEPANGTNWFYYGENYFKSDNLDSAAIAYSKGLQAEPGNPINLVGTGKIKLEKGLNS
                     EARADFDKALTMSGSKTSLVQSEVAESFIRTKNKDLSYAIKLLNNAIAVDSKNAELYI
                     LLGDAYSEQNNGTLAAENYNRALDLDKKAVKAIVQKGVLYKRSTNYEGAAAEFQSAIK
                     LNPDFAPSYRELAETYFKMRKLEDAKQSYRKYLELSKNNSMARMRYASFLFMSGDYAD
                     AQSEMNQLSSIDSSNIGMLRLSSYIAFEMNDIPKAKMLMEKIMNTTIENQRTAMDYEY
                     SGKILLKEGNDSLAAENFEKSYQIDTTKTEILQDMGSMYMKLKKYPQAVNAYERRVNN
                     GKGLTSTDYFNLARAYYFNKDYTHADTLFAKVIELLPTWPNGILWRAQTNAQLDPDSK
                     GGMAKPYYEKYIEVALADSANADKYKNGLVESYKYLGSYYYLIDKKI"
     gene            complement(6280..7230)
                     /locus_tag="PKY09_02595"
     CDS             complement(6280..7230)
                     /locus_tag="PKY09_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNB11773.1"
                     /translation="MKVKNFLLLVLSTAIALHLASCGGDAFKPDLSDTPTSGNLKIIS
                     DESFEPLMKSQAETFMSLYKNAKIEISYKTEADAINYLMNDSVKVIISGKDLTPEQHQ
                     YFKDHKVLARSTKIAIDALALIINKDNIDSLLTLSQFESLITGKTNNWSAIDNKNNLG
                     EVAIVFDKNGSSNVRFLKEKLLQSGEFPANCYALNSNKEVVDYVINKKNAIGIIGVSW
                     ISDSNDSTAMTFLNQIKVVALKNDTATEYTDYYQPYQAYIALKQYPLTRDVFLINRQS
                     RNGLGTGFASFVAGDQGQRLVRLKGLLPATMPVRIVRTGN"
     gene            complement(7220..7432)
                     /locus_tag="PKY09_02600"
     CDS             complement(7220..7432)
                     /locus_tag="PKY09_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11774.1"
                     /translation="MIRKVGIYFNLLMIAGYAVMGFFLIFADNLDLLDQPQQKWLGGI
                     LVVYAIYRGYRSFRSSKKEQEPLDES"
     gene            complement(7445..8251)
                     /locus_tag="PKY09_02605"
     CDS             complement(7445..8251)
                     /locus_tag="PKY09_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB11775.1"
                     /translation="MATSFFNNWDDPTFAKRNELVFDGRNKSYGAYLIRQLYSKRINI
                     AFVIAAAGLIIFISLPLILELMGDKATENVVQKEVEVTLSEPPPVDPAEPPPPPPPPP
                     PPVVQTIKFTPPVVVDKPVEEEQPPPQEKLSETNVGVVTQEGDPNATELPPEPVVADP
                     NEGKIFTIVEEMPQFPGGGEAALIKYLQNNIKYPAMARENGIEGIVYVTFVVDKDGKV
                     KDAKILRGKGAGLDDEALRVVRSMPDWKPGKQNGRSVAVQYNLPVNFKLQ"
     gene            complement(8254..8865)
                     /locus_tag="PKY09_02610"
     CDS             complement(8254..8865)
                     /locus_tag="PKY09_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB11776.1"
                     /translation="MAEVNTDQGGGKEKGGKHSKVRAKKASTHIDMTPMVDLAFLLLT
                     FFMLTTTFSKPKTVEINMPLKDGEPTKVNNAITVLLSDKDKVFWYFGEFKPDETQLNQ
                     TDFSDNGIRKILLDKNAAAHAEVKRLEDELAKRQIADSTFKRLAVEAKSQKSALMVLI
                     KTDDKAKYRNVIDILDELSIASVGKYAVVDMMKDEYDLIQKMN"
     gene            complement(8872..9519)
                     /locus_tag="PKY09_02615"
     CDS             complement(8872..9519)
                     /locus_tag="PKY09_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB11777.1"
                     /translation="MPKIKIPKKSPSLDMTPMVDLAFLLVTFFMLTTKFRPDEPVIVD
                     TPASVSTKILPENTLQVTIDSAGRVFFNIEGQNVRRNLMTRISEKYKFQLTEADLKKF
                     SLMTTFGMPADKIKTYLEAPDSERKKMDKATGGIPIDTLNNQLADWIVFGWTAAITDE
                     GYEARKQAGNELRFAIKGDGRVDYTYIKRVIEIFQQNKINRFNLITNLQATPVQE"
     gene            complement(9550..10353)
                     /locus_tag="PKY09_02620"
     CDS             complement(9550..10353)
                     /locus_tag="PKY09_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB11778.1"
                     /translation="MSSSNNSGGGFKSIFAAIVIPAAILVAILIYAYILGNPANFMEN
                     DPNNHPNNYLGIIHKGGVIVPILISLLLIVITISIERMLTIGASKGKSSVDSFVGKIR
                     SLLSKGDLNTAISECDKQKGSIANVVKAGIESYRKMQGETALDKERKTAAIQKELDEA
                     TTLELPMLSKNLVIISTVASVATLMGLLGTVIGMIRAFAALAQAGAPDAIALATGISE
                     ALINTALGVGTSALAIIMYNYFTNKIDSLTYGIDEASYSIVQSFTANTK"
     gene            complement(10518..10700)
                     /locus_tag="PKY09_02625"
     CDS             complement(10518..10700)
                     /locus_tag="PKY09_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNB11779.1"
                     /translation="MNESKRLVKGEKKLFGVCSGIANYFEADPTLIRLLFIIAVFGFG
                     TGVLLYIILAIIMPEK"
     gene            10851..11285
                     /locus_tag="PKY09_02630"
     CDS             10851..11285
                     /locus_tag="PKY09_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNB11780.1"
                     /translation="MRAITFILAVVVLTSSGCLNLKMPQVTGISKLRLSDITKDTRIQ
                     FDVGLRNPNAFGVTLKSMKAELFLADSSIAGIGVDRKTRLAANQHVDLPFSVQPKLTA
                     LPQLGILGITQLFKKDNKKISLRGELKVRKFIFTKKVKFSIP"
     gene            11299..12333
                     /gene="mnmH"
                     /locus_tag="PKY09_02635"
     CDS             11299..12333
                     /gene="mnmH"
                     /locus_tag="PKY09_02635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNB11781.1"
                     /translation="MISVIPIDQFLNNHASHVALLDTRSPAEYEHAHFPGAVSFPLMT
                     NEQRAIIGTIYKQEGKDAAVLKGFELIGPQFAEFVRQANTLVSDKNVALYCWRGGLRS
                     SVMAWVLSTAGFKVTILKGGYKAFRQWVLSQFLIKKPMIVVGGKTGAGKTEMLYHLQS
                     EGQFIIDLEKLAHHKGSAFGALGMSAQPSSEHFENQLALQWHQAPFSQFTWIENESIK
                     IGKCALPVNFFDQMREAKCFEMDVSLDVRIERTLESYGKFDVDLLSDSTMKIKKRLGG
                     LRLKMALEALEQKDLKLWCSIVMEYYDKTYQHSHNKRQENEIVKVDAEGMDKSTIAKE
                     MIKLSKQVVL"
     gene            12330..13364
                     /gene="selD"
                     /locus_tag="PKY09_02640"
     CDS             12330..13364
                     /gene="selD"
                     /locus_tag="PKY09_02640"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNB11782.1"
                     /translation="MSEIVKLTQFSRQGGCGCKIAPEKLSEIIHDKESVVFKQLLVGN
                     AESDDAAVFDLGNGTSVISTVDFFMPIVDDPVLFGSIAAANAISDVYAMGGTPVMAVA
                     ILGFPINKIPLSVAKEIKAGAEKVCHDAGIPLAGGHSVDSLEPMFGLAVTGIIPTENV
                     KRNSTAREGDFLLLTKPIGAGVITAALKRGVISEGHLEQAMKNMTTVNAIGKELGRLK
                     CVNAMTDVTGFGLAGHLKEMCTPGNLSVEIYFDKLPVMDGVREYISKFIYPDMTTKVY
                     SSIASQCNELNSEQLFISCDPQTNGGLLVAVTPDGLNEVLSIFDTYNIPYITPIGRFL
                     SRQEKMINII"
     gene            13493..15403
                     /gene="acs"
                     /locus_tag="PKY09_02645"
     CDS             13493..15403
                     /gene="acs"
                     /locus_tag="PKY09_02645"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNB11783.1"
                     /translation="MYPYQITSEEQYTNDYRNSVENPEKFWADIAQYFSWKKTWDKVL
                     SADFEKAEVKWFAGAKLNITENCLDRHIEKLASQPAIIWEPNDPNEHYRVLTYKELLF
                     KVKQFANVLKNNGVKKGDRVCIYMGMIPELAIAVLACARIGAIHSVIFGGFSARSIAD
                     RLQDADARFIVTCDGAFRGAKDIPLKAIIDDALESCPFVQRVIVCTRTHTPVSMIKGR
                     DVWWEDEIKIVETQGNPDCIAEVMDAEDVLFILYTSGSTGKPKGVVHSVAGYMVWTNY
                     TFVNVFQYTPGQIYFCTADIGWITGHSYLLYGPLSAGATTMMFEGIPTFPDAGRFWDI
                     TQKHKVDILYTAPTAIRSLMAYGTSYLEGKDLSSLKVLGTVGEPINEEAWYWYYENVG
                     KSKCPIVDTWWQTETGGIMISNLAGVTPHKPTYATLPMPGVLPCLVDETGNELTDNGV
                     SGNLCIKQAWPGMIRTTYGDHERCRQTYFSTYKNMYFTGDGCLRDKDGNYRITGRVDD
                     VLNVSGHRIGTAEVENAINMHQGVVESAVVGFPHDIKGQGIYAFVIFNGMHVDEEHTR
                     KDVIQTVSRIIGAIAKPDKIQFVSGLPKTRSGKIMRRILRKIAEGELNNLGDTTTLLD
                     PGIVEEIKSGRL"
     gene            15506..18154
                     /locus_tag="PKY09_02650"
     CDS             15506..18154
                     /locus_tag="PKY09_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNB11784.1"
                     /translation="MNRVVVSSIISIVIIGLMAVGYYYFSAYREKFSATEDALPNDAA
                     IVIRGSIGDLYTSLEKVGVWNEKDSLTVMYRFKKEMHTLLSNTTSMFDVADLLESPQT
                     TIAVLATGARNAELLYLVPSRKELNIDALQKALTGQMSKNKSRTFAGVSIYEIQFSNF
                     KSSFTFAFANGIFIGSFNAGLVEDAIRQQATGKPFGGNVSAQKLYKEVSVSTGAILGL
                     NYPALKKLFSVAISSDQFNELANLEKWAEWSFHNLTFDGNSINASGTVLISDSTLQIS
                     DFGNARSSADLLWSVLPDDVSALQIISFKDAQIWSDKNKRRNADNEEIINFIKQVSAT
                     ETKFKVEVKKDFATIVGGIGAVAVKGNPTSLLENNSIALLQLKDQKKSLTQLLSLSKK
                     LSKNKEVNAVEKFRNHEIRFLNCDGLLPAMFGNAFTVIRKNYFTTHNGFIIFANKPSQ
                     LRGFIDDVEDKEMFNKSEVYQQNKAVLSEGMSYALFLRPTNLNSYFQSVANSSTLKAL
                     AATDLMYKLSSFYYYIQPGVSGKAECNFRVVIQQKQEQKVSEPKWSFFADSAIQCGPF
                     IFNSDNGISILFQDSTKSLICLDESGNVSWKTTINGFINGKISQTVNDQLGLLQLVCS
                     TDDSIYMFNQNGVLPARYPFKLPVQSRNGVVASPNANVLFVAGENNLMYVFDPGSRPS
                     QNWNTVQLNGNLFNASIDASGRFVYIMDGDKLRLYSTDGKLILSMPSAEYKVNSIQWV
                     NDSVPSLFMLNNKGNIVVWNTDGTYKIASGDSAFDMAATCGTGTARMWYTIKGKELKI
                     LDYTFKAIQNADLSKIVFNAMQLFDTGNEILCILKDAENIAIYRGDLKILPQVIPAVS
                     FAVKTFQGKTYLISRTGNGKLILYVL"
     gene            complement(18151..19674)
                     /locus_tag="PKY09_02655"
     CDS             complement(18151..19674)
                     /locus_tag="PKY09_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB11785.1"
                     /translation="MRKFLCLILLTSLTTFQTFSQDNFIKDSLDSYINREMQKWQIPG
                     VAIAIVKDGKVLAMKGYGVREIGKPDKVDEFTLFQIASNTKAFTGTALALLANQKRIS
                     LDDKVTKYLPWFQLKDPCATNMVTIKDILCHRIGFYTFQSDFLNWDCNMTTDALIKNM
                     RNVQPVNEFRDKYGYCNMGYVTAGEVIKAVTDTSWQDFFDYHFFKPLQMNRSSVYHQK
                     IVTDVNAAKPYTLVDNKIVLLDYANIDNIGACASINSCVNDISHWIMMQLNKGKYENK
                     EVIPSAVINTTQNSYMIVRESGSARMPYNHFQNYGLGWKSEDMYGYKIVSHDGGANGF
                     VTNTTLLPELNAGFVILTNTDANWFYGALRQQLINYFAKQAYTNVSENYFGIYSKNNI
                     TESEEIQNLKKQANAEVHLSVADAALVGKYFNEVYGEIEIKIEKSGLMMYFSHHPKLR
                     GALKPLSEKSLLCTFNDPTYGIHPFPIEVVNGKVNNITVKVNDFIDYQYYKFTKQNN"
     gene            19878..20537
                     /locus_tag="PKY09_02660"
     CDS             19878..20537
                     /locus_tag="PKY09_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNB11786.1"
                     /translation="MFNTEKYKKLCEELLPYKASLIAVTKTKPVSDIETAIQNGQKLF
                     GENYVQELVEKVPVLSKNIQWHFIGNLQRNKVKQIAPFIHTVQTVDSKKLLHDLDKHA
                     AANNRVIKILLQVHIAKEETKFGFSVPEVQMLIDEGVFQQYNNISFNGLMGMATNTDD
                     TSQVRSEFKQLYGLYKSISGAFDRSESPVLSMGMTSDYKIALDEGSNMIRIGSALFGS
                     R"
     gene            20542..21498
                     /locus_tag="PKY09_02665"
     CDS             20542..21498
                     /locus_tag="PKY09_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB11787.1"
                     /translation="MGLLFALVTTLSWSIGIFPFTEASRRLGINSLNHFRLFLAVILL
                     FVTAFLINHSEFINLFNAQYVQAWIWFGLSGVVGLALGDYFYFGLYAIMGARIGSIFS
                     TLSPAAALVTSYFFVDERINFIGVIGIFLTMAGVVFISVSKSESFSAEKGRYGSDVKG
                     VLFGVLAAFCQGVGLVLAKKGFVVLEQEGKFIHPINATFVRMVVSLTTLVLITTIFRK
                     WSDVLRPLKSNSAGLKYAAVGTFFGPVFGVCMSLYTITLLEATVAQTIFSLVPVVAAL
                     IARYFYKEKFTLASVIATLIAVAGVVLLIRRNEVLECMYKIN"
     gene            21502..24489
                     /locus_tag="PKY09_02670"
     CDS             21502..24489
                     /locus_tag="PKY09_02670"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB11788.1"
                     /translation="MRRIILLVFGFVCIVKIAIGERPYFQQHVDYKISVTLDDKSNFL
                     TGFEEMIYRNNSQDTLRELFFHLWPNAYKDRNTALCKQLVSQGNMNLYFASDEQRGFI
                     DSLEFKVDDKGVTVVYDSINIDICKIVLNKPLLPNGTVIITTPFRVKIPDAKFSRLGH
                     VGQSYMISQWYPKPAVYDRDGWHAMPYLSIGEFYSEFGSFQVDISVPANYVVAASGYL
                     KTPSEVEFINNKIKTEVNEIDLDKRNTFPESEREFKTIRFVVDSVHDFAWFADKRFRI
                     VSKSIELASGRKVDCRAYFTPANSYIWHRAADYVSEAVLFYSKYVGEYPYDVCTAVDG
                     TIAAGGGMEYPTITVIGNTGNSIVLNQSIAHEVGHNWFYGLLANNERELPWMDEGINS
                     FYEQLYMTQYYAKENSVAYQLIGVPYKAAKIFGADKISFSGFNKISYLLTARTNDDQS
                     SGLTSEYFSNLNYGTDIYSKIPLCFSQLREYLGTEKYDSCMHTYFNIWKFKHPDEAAI
                     KNVFETTSGKSLDWFFNGMINSNAKGDAKICKVHADAGGTDVLLKNKGNLAMPVNVSF
                     YNKEKLIASQWTEVFAGKFKLSSTVVGADKIVLDTNDESLDLSPFNNSIKTKGIFRKW
                     KPLQLRFLTMLENPERIQLFYLPAVAYNNYNGWMTGVILHNYSLLNKKFEFGIAPMYA
                     FKSKSVAGIATASYQFNIYKGWFDRIVAQVSPSLFDEGLSENKKVFKGYFSIPASLSF
                     RVRKSQYKPYSEKYVNVTHQYVHQTFSTQSNLAATNAINITQLEFASYNYNKTDPGSY
                     SFILERGIHYVKLYGTWNKEITLTRRKKDLSFRFFAGTFLDNTGNGGEANFRSSAWRG
                     TDDYSYSWLWFGRNEYDGFWAHQMQKSDGAMAAYYPVRTDHWLAALNVSVKTPVPGLR
                     LFADGNTFYNANKVSPGATVIRYDAGIMLSVVHDAFEIYWPLIVSKEVRDYYDLNDIK
                     LTDRIRFVFNINKLNPVLLRKMR"
     gene            complement(24495..28931)
                     /locus_tag="PKY09_02675"
     CDS             complement(24495..28931)
                     /locus_tag="PKY09_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11789.1"
                     /translation="MNTTIKHITILLALVLINSGSINAQCSGGSNAGAITPSINWQTR
                     AVDGREFLTFPATAGYKYIFSFCAADGGSTSYNTEITINTNAGVAIPGAYNNDFCGTA
                     SYLEWTCVTTGTYRVLTTRTFCNSRTNLGTLAYRYDFSYSCPAGLGTGYTTIASLPYT
                     MASGTTCGQVNDVNSIRLTACGTANYYDGEDMIWSFTPTTSGQITVSYNSGANNTILS
                     VFDACPLTGNGANCVSTQQGNGNKSLTFCITAGVTYYVVMDCRLSTGCFAFTNFSITA
                     PTTPSCNLGGGVINVASLPYVATQQSTCGNGNNLTDGNTPTCGSTNYKGSEDNVYIFT
                     PATSGDISIRLKTTASRNGMFLYDGCPLATPCGPSTANCIGAQTGNDDKTLCATVTAG
                     TTYYLLIDSRTSCFAFDLSISAPSTAVVGRTCASPLVIASLPFKRYNETTACFGDDYN
                     NSSISSCGTNYESGEDKVYSFTTASARCISVTLSSASTNYIGYQVYNGCPDAPGTSCV
                     ASLGQATSGFLSGTVTLPSAGTYYIIVDTWANPENADYTISIANVSSVYNDLPCNAQS
                     MELGLYYTGNSNCADGSGEPAAPGCWTTPNVVNSVWYSFIAPASGNVTVRTNPGTLRR
                     TQMAAYSGVCGSGMTFIACNLNAPGCGGTSPTPTTMSQLSLTGLTPGNTYYVVVDGEG
                     YLTGTFSIIAVNGGTTLPEIEGQDCGSPIIVCNQNIQVGDPGFQLFGNNCDFPGAGTN
                     CLYTAERGSAWYQINIASAGNLEFNIIPNDWVGAPSTACTDYDFAVWKIAGSGATTCA
                     GIAGGATPLRCNYSSLGLTGLYSNSNGTAPAAYPGFNGAYNSRISAAAGDVYLLVVSN
                     YSNSISGFTLNFPASAPVDYTAGGTTAYWTGAESNDWFTAANWGGCNIPSCTVNAVIP
                     LSGANQPYIGTAGASCKDLKLEPGGTLIMDNNQFLTICGNVQNNGELNIATGSGFLFN
                     NGSVDQTVNGNLISPNRFSHITVSKTGGKVDLLQNIEMQGSLALIGPNSRFDAINKTV
                     ILKGSLSNSAGQFNMGNNGNLVLNGLGTQNINNNDTLTNVVVNKAGSTVVLISDMKIG
                     PTGTLSLLASRIQTTASHEVYVMNDASSAVNAGSSGSYIEGYLRRNVPAVSAPRVYDF
                     PVGNTTKGYQRLSLDLYNGINPAVGSIQASFSNYSTTIWGLGLDAPCLMNYNDTMLDN
                     GYWTLTPANATNAEANITLYNRSYTNAKTNYGVGINPNGTGWIIPPVISGGCVTPPVT
                     AVLRNGVGLSFASGQSILVGTAQGVVSPLPVELLSFEATAGKQSIFLTWSTASEKNNA
                     GFYLERSTDGTDFDNVTWVDGHGTTNQVSNYSFEDRNVKEGVLYYYRLKQIDYDGKFE
                     HSFVKTAMIGDLKNVILNAAPNPYRGSTKINYTIPTDGTVVILITDMTGRILKNIDAG
                     LQSKGSYSIPFSAIADGHPSGVYNVTLFFNDKPYRLKLSEYR"
     gene            complement(28934..29299)
                     /locus_tag="PKY09_02680"
     CDS             complement(28934..29299)
                     /locus_tag="PKY09_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11790.1"
                     /translation="MKKLVFAVMIMLLSKAAYCQEKISAQKSEEFVFEIPQLDLVKQL
                     ADFKQNLKQIKGLEFHGFCESKHLLMVSMPTASKDEFYDLMDKMQYSALEKKQATFRL
                     AEQVCNAKEEINYSKSINQ"
     gene            29490..30242
                     /locus_tag="PKY09_02685"
     CDS             29490..30242
                     /locus_tag="PKY09_02685"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNB11791.1"
                     /translation="MSGTGKKIYFISDFHLGAPTSEESLIRERKICQWLETIKTDVSI
                     LFILGDVFDFWFEYRHVVPKGYVRILGKLAEIADNGVPIYLFTGNHDMWMFDYLPKEL
                     GVKLYRQPKEFVFDNKKFLIGHGDGLGPGDHGYKFIKKVFANKFCQWLFARLHPNFGI
                     AIANFWSRKSRQHTGHHDAKYLGDEKEYLVQYIIETEKTSHFDFYIFGHRHLPIDKQI
                     GDSHYINLGDWLSYFTYAEWDGSKLSLRKFEA"
     gene            30254..32098
                     /locus_tag="PKY09_02690"
     CDS             30254..32098
                     /locus_tag="PKY09_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HNB11792.1"
                     /translation="MKKFNALQFGAWFFLAISILAIVVSFRYLKYVSTGGDWLTIIYL
                     TATILSHFSALSFLIYGLLFVPVALLCKKRQVLIWYAVVIGSVSLLVLILDTFIYDLY
                     RFHINGFVLDMVFGGAFSEIFVFDLVMYLKVAAFVVLLTAIVYLLTRIIWSLFERKKL
                     RGGKQIAYLLAGLLIGSHLLHAWAYATSYRSIQQASLYFPLYFPLRANKLLSTLGVAD
                     MKQKQEWENLQDGEDKAIQYPLEPLTFDTATNYPNILFILMDAWQFETFNHEVTPNIY
                     RFSQGKILFNRHYSGSNGTRGGIFSMFYGIPSLYWEDFLINRISPVFLDELQKRNYEM
                     KILASAALVSPEFDKTVFSKINNLRTHTPGKNFTERDENITKEFIQWMNTSDFQKPFF
                     GFLFYDSPHNYNLPYWLPHPFKPYWENVDHTVLNNDFNPVPFYNSYKNTVWYCDSLIQ
                     KVLSKLEEKKLLENTIVVITSDHGQEFNENKKNYWGHTSNFSKYQLQVPCIISWPGKT
                     AETVSRFTSHYDLVPTLMHDVLKCRNLFSDYSFGRNLFDTTAQRVFVAGSKEHFAFID
                     LQKNQINTVNFNGSMTVTDMRLNELNVKPDAKMLNDCVLNLQRYYKSR"
     gene            complement(32095..33000)
                     /locus_tag="PKY09_02695"
     CDS             complement(32095..33000)
                     /locus_tag="PKY09_02695"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNB11793.1"
                     /translation="MIRPTSFGFNIETSMDNVFQKQNHRLSNQQIQLQAEKESSLLRK
                     KLIDNNINVIYAYENCESATPDALFPNNWFSTHDNGTLVLYPMLAANRRKERNASLIN
                     YLKNSFQIKETIDLTQSEDENKFLEGTGSLVFDHKNKTCFVSISERTNIELATNINNK
                     LAYQTTILNYTDENNIPVYHTNVCMCIGDGFVIIATDNLNSKSEHTTLFEYFEANNLD
                     PVLLSSKQVHSFCGNALQLQTTNGQPVLAMSNTAFNAFSTEQIKTIEKHSDILHTPID
                     TIEQIGGGSVRCMLAEIFLKPKNSF"
     gene            complement(33030..34022)
                     /gene="gap"
                     /locus_tag="PKY09_02700"
     CDS             complement(33030..34022)
                     /gene="gap"
                     /locus_tag="PKY09_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNB11794.1"
                     /translation="MTHVAINGFGRIGRTVLKKLLQNKNLIIVGINDLTDPKTLAHLF
                     KYDSIHGKFDGTVSASEKAIIINNQTIPVYAEKDPAMLPWKELQVDVVIESTGRFTKA
                     DLAKAHIKAGARKVIISAPGSGNLKSVVLGVNENILDGSEEIISNASCTTNNAAPMIK
                     ILQDNWGVESAYITTVHAYTGDQNIHDAPHKDLRRARAAAQSIIPTTTGAAKAVTEIF
                     PDLKGMIGGAGIRVPVIDGSITDITCILKHDATVEAINQKFLEASQGKMKGIIEYTTD
                     PIVSVDIIGNTHSCIFDSVLTSVLGKMVKVVGWYDNEMGYSTRLAELTALVGKH"
     gene            complement(34035..34907)
                     /gene="lipA"
                     /locus_tag="PKY09_02705"
     CDS             complement(34035..34907)
                     /gene="lipA"
                     /locus_tag="PKY09_02705"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNB11795.1"
                     /translation="MEEKAKERLYKKPEWLKVKLPIGEEYRKVRGIVDNNKLHTICES
                     GNCPNMGECWGAGTATFMILGNICTRSCKFCAVATGKPLAVDKTEPQRIAESVKLMKV
                     KHCVITSVDRDDLKDGGSEIWAETIRKVREISQGTTMETLVPDFAGKWENLERVMQER
                     PEIMSHNLETVRRLTKEVRVQAKYDRSLEALKRINSFGLRTKSGVMLGLGETREEVLE
                     TMNDLYQNGVHILTLGQYLQPTKEHLPIAEFVSPAVFKEYKQIGLEMGFKYVESGPLV
                     RSSYHAERHLTFDV"
     gene            complement(35023..35096)
                     /locus_tag="PKY09_02710"
     tRNA            complement(35023..35096)
                     /locus_tag="PKY09_02710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35060..35062),aa:Ala,seq:ggc)
     gene            complement(35217..39770)
                     /locus_tag="PKY09_02715"
     CDS             complement(35217..39770)
                     /locus_tag="PKY09_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11796.1"
                     /translation="MKRLFTTLSLGLATMIGFAQSNCYFSEDMETIDSVTSTPANAWV
                     QNTRISVSGVACDSAQVLNGTDAILETDPIDLTGFFFVSVTFQHICRIDFFDAANIDV
                     SIDGGMTWSTLGPAEFDSTLADYGAFGGQGYFSSATSLDWDPGVPGTMPTNSMWHLAR
                     FNLSQWGGVSDFRMRFRLSDVNGGGAGGYPGWFIDDICIQAAVCELNPPNIAFTSGYP
                     QGTIYNLGPYNVQSHITDASGIMSATLTYTINNGTPVSVPMTDLGNGDFTGQIPAVND
                     GDSICYSITALDNSGCANSAVFPTSGCITFYASQGITFPFCDNFDLQNLWTDSVGNAN
                     STHWQRGQSTVWPGSAHSAPNIWEVGLDQQYVGSCISYLYSPTFSFIGVFNAQIEFWI
                     NSNCENFWDGARLDYSTDNGLTWSVLGTNNTTFPTGQNWYNDDQLNSSQTYAWTGSSQ
                     VISGAPGWFKAMHKLTVLDNQTSVMFRFVFTSDASVQLDGFAIDDVCIFVPPPQDAGV
                     QIISQPPVQAPAGLCIPVEVTITNFGSQPITSTPVTYTSSTGATQTATWTGNLAPGAS
                     TVFTITPCLTIPSGPFTVCAYTSLPNDGLPFNDTTCISSTGIPVLTLTSCDDFESGNV
                     GWLSTPTGGANEWQLGTPAFGATTGAYSGTNAWDINLNTGYVQNEIDTLYTPIYDITG
                     LSASNLTMSFFSNYSTANTDGFWVEYTIDGTNWSILGTMGDPNASNWYTTANLDFTNN
                     PAFNNNSGGWKKSTYFLNNLLITNPTTIRFRFVFYATTFGTGGVGVSLDNFCVSIPCS
                     PDAGVTAVGSYNASIGNTLMQGSADSVQVTIHNYGTAAVTSLNIAYAINGTPVLPPYA
                     WTGNLAPNGNATIILPTSYTTPGGQYTITAWTDLTSDCNSANDTLAGAAVGVPVIVVD
                     FNNSYCDDFENGNIGWSTKNNSTTPGSDWQFGTPTFGTTNSAYSGVNCWDINLNSVYG
                     ANANSELYTPIFDLSNAVDATISFWQNVASESNWDGTRMEYRIGNGAWTILGTPCDTL
                     TMGQFNWYNDNQLNSSQQYAWTQGNALCALPTGWIRSMRKLEPIFNNQPMVQFRYIFN
                     SDGSVQTNGYSIDDFCVNVPVPLTVTPVAVSTANPGPPFIFPGQSMPFKSKIYNDGTS
                     AVNNFVATLSVDGNIISNDTVTLATPLNPNTNMNYTFVNGWIASPGFHNVCVSTYYPN
                     QSADLKPIGDTICYSVQVFDSATVGAGSSSKCYDFESGYRWVTLNSTSYSANSSWDLG
                     TSVKLGGNHSPVNSWFTSLAGQYPNRDTSALFSPVFTLTGGLTYNINFWHSFMTERNE
                     DGGTFEYSTNLGATWIQLGDDHDPNWMNTYSITALGPVPPVHPGWSGNSGGWGQVGHD
                     ICLPTAGQQQVIFRWRFNSDFSVRDEGWAIDDVCLEVKSPITLCTTGLTDLNNTFDMI
                     QNTPNPFSDYSVITFNLSDNGSTQLDIVDVTGKVLMTPVNSNLSAGTYNITVSAENLA
                     SGVYFYVLKHNDRQLVKKMVIAK"
     gene            40125..41315
                     /gene="sucC"
                     /locus_tag="PKY09_02720"
     CDS             40125..41315
                     /gene="sucC"
                     /locus_tag="PKY09_02720"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNB11797.1"
                     /translation="MNIHEYQGKSILKSFGVAIQEGIVAETADQAVAAAQELQKQTGT
                     NFWVVKAQIHAGGRGKGGGVKLAKSIDEVKEKANAIIGMQLVTPQTGPQGKKVHKVLI
                     AQDVYYPGEAKTAEFYMSVLLNRQAGKNIIMYSTEGGMDIEEVAAKTPEKIFKEEIDP
                     AVGLQPFQARKIAFNLGLSGNAYKEMVRFVSALYKAYESCDASLFEINPVLKTSDNKI
                     IAVDAKVNLDENALFRHADYAAMRDKSEEDPTEVEASEHDLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLAGGEPANFLDIGGTANAARVEQAFRIILKDPNVKAILVNIFGGIVRC
                     DRVAQGIVDAYNNIGQIPVPIIVRLQGTNAEEAKTIIDKSGLKVYSAILLKEAAQLVK
                     EKLG"
     gene            41319..42212
                     /gene="speB"
                     /locus_tag="PKY09_02725"
     CDS             41319..42212
                     /gene="speB"
                     /locus_tag="PKY09_02725"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNB11798.1"
                     /translation="MKALSTKHNFLGIEDEKYYNYTASKYVIQQVPYEHTSSYLAGSD
                     KGPAAIVKASQYVELYDEELDAETYKTCGIATLKALDFKNKTNKKAVDYIEKETLALL
                     NDKKFVVSLGAEHTVTLGFVKAHIQKYKNVSVLQIDAHSDLRESYHNNIYSHASVMKR
                     IYDLGLNIVQAGIRAQCIEEAQLIKKSKRLHTYYAHNIRKDAKWIDKAVKDLSENVYV
                     TIDADGFDPSVIPSVGTAEPNGLFWNETLALLKKTAKEKNIIGFDIVECAPTKGNILS
                     EYTLAKLAYRIIGYIEAARKE"
     gene            42232..42891
                     /locus_tag="PKY09_02730"
     CDS             42232..42891
                     /locus_tag="PKY09_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB11799.1"
                     /translation="MENRKISNKGSATLFQNRLLERLTRTTFYFPVILFLVVGIVTVL
                     YCYLSLKINILSIVALYLAGLFAFTLVEYLIHRFIFHFKANTPGQELLKYKIHGVHHH
                     YPKDKDRLAMPPFMSLTLAALFFLLYKWLFGNYGIALFSGFVSGYSVYLIIHYAVHRY
                     RQPHNFLSILWKHHSLHHYRSDNSAFSVSNPLWDYVFGTMPDKKTIQKGDKEKLPDYF
                     T"
     gene            complement(42936..44723)
                     /gene="lepA"
                     /locus_tag="PKY09_02735"
     CDS             complement(42936..44723)
                     /gene="lepA"
                     /locus_tag="PKY09_02735"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNB11800.1"
                     /translation="MENIRNFCIIAHIDHGKSTLADRLLEYTNTISKQQMQAQVLDDM
                     DLERERGITIKSHAIQMDYTLDGKKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     AAQGIQAQTISNLYLALEHDLTIIPVLNKIDLPSAEPELVKDQIVDLLGCRREEIMAA
                     SGKTGMGVHDILREIVHRIPAPKGNPKAPLKALIFDSVYNSFRGIIAYFKVVEGEMKS
                     GDKVKFVATNSNYIAEEIGILKLDKIPAKTIGTGNVGYIITGIKDAKEVKVGDTITTL
                     ENPCAEAISGFEDVKPMVFAGIYPVDTEDYEELRSSMEKLQLNDASLVFEPESSAALG
                     FGFRCGFLGMLHMEIIQERLEREFNMTVITTVPNVSYVSYLTNGDVYEIHNPSDFPDP
                     SKMDHIEEPYIKAQIITKSEYIGAIMTLCINKRGVIIGQNYLTTDRVELIFEMPLAEI
                     VFDFYDKLKSVSKGYASFDYHPIGYKTSDLVKLDIRLNSEPVDALSALIHRDHSYTFG
                     KKICEKLKELITRQQFEIIIQASIGAKIIARETVKAIRKDVTAKCYGGDISRKRKLLE
                     KQKEGKKRMRQVGSVEIPQSAFMAVLKLD"
     gene            44851..45369
                     /locus_tag="PKY09_02740"
     CDS             44851..45369
                     /locus_tag="PKY09_02740"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HNB11801.1"
                     /translation="MEELRYPIGKFQLPGIITSEQFSSWKNDIVALPSKVQQLTDGFK
                     AHHWSQTYRPDGWTALQLVHHLADSHMNCFIRFKWSLTEENPTIKAYDEKAWANLPDV
                     TQSSPMISIQLLHALHQRWINLINNMTEQDFEKTFFHPIQQKTHSLKGAVGLYAWHGN
                     HHLAHLKIIADK"
     gene            45456..47453
                     /locus_tag="PKY09_02745"
     CDS             45456..47453
                     /locus_tag="PKY09_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11802.1"
                     /translation="MRQKLIALSVLLLIVLLLNFCTTQKKQTLPLLSSYASLNDTVKY
                     VGKETCRSCHAEIYNTFMQTGMGKSFDVASKQKTSAKFSHAVIYDKYKNFYYHPFWQN
                     DSLHLLEFRLENGDTVYKRNEVINFIIGSGQHTNSHIINTNGYLNQAPATFYTQKGEW
                     HLPPGFEDGNNTRFSRIIGLECMSCHNSYPDFVLGSENKYNKLESGINCERCHGPGEY
                     HVQQKLAGILVDTAKSIDFSIVNPAKLSIDLQFDVCMRCHLQGNAVLNSDKSFFDFKP
                     GMKLSDVMNVFVPLYEGNGNAHIMASHAERLKQSRCYIETANKNHVNHQPSELRPGKN
                     ALTCITCHNPHVSVRQTNDDHFNAVCKNCHHTENSLCTEKLAVRSKSNDNCISCHMTF
                     SGTSDIPHVSIHDHKISIPSKDNTAHEHKVFKGIYCVNNPAADRKSRGRAFIAYFERY
                     GYDTSVLDSALNCFSDAGNVSLRENINELVQIYFLKNSFAKVIELTSAMPDMLKLLNK
                     KSYSNTHAWTAYRIGASFQAIGKIEAALPFYKRAAELAPYHLDVLNKYATALSIAGNV
                     KEAQKIYEFILKEYPKHVSALTNLGFIFLSEKNDPQQALAYYNKALALNPDYEPALMN
                     MAGLMHYMNRNKEAIEFVKRILKISPNNEGARQLLNQLKST"
     gene            47454..48482
                     /gene="mltG"
                     /locus_tag="PKY09_02750"
     CDS             47454..48482
                     /gene="mltG"
                     /locus_tag="PKY09_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNB11803.1"
                     /translation="MKKILFLVLTLLLLAAAVVAYIFYQRIFSPNVKLKDHKTFFYIR
                     TGSDFNQVATELTDQHILINTESFKWLAERMNYTERVIPGRYEITDNMNNRQLLQLLR
                     SGKQVPIKITFNNIRTKSDLASRISKQIEADSTQIAAMLSDSLLLKEEALNSDNVLCL
                     FIPNTYEMYWNTSATDFFKRMKREYDNFWNTSRRQKAEQQGLTPVQVSILASIVQSET
                     KQNAEKPRVAGVYLNRLRQNWKLEADPTLVFALGDFSIKRVLNEYKTIDSPYNTYKYF
                     GLPPGPICLPDISSIDAVLNAESHQFMYFCAREDMSGFHSFAKDYNTHLLNARRYQAE
                     LNRRNIRN"
     gene            48516..49619
                     /locus_tag="PKY09_02755"
     CDS             48516..49619
                     /locus_tag="PKY09_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11804.1"
                     /translation="MRKGFLIVIAALFITADVSANQVAALLRVKRSEQPDTLGFNLVE
                     QLTKITYQLIQQNKIKLWDSPSKEIQITAASLQEIEQSSSTSFKEEDVIYIYESWTEA
                     KREIQTKTIGIGFSNRDSRNQEVSYGYVDYADLVRFLNEKPAEVNANGNYGETPAFYL
                     SAKKFNFSLVQFNNKVIQSADESQKTLKSFVGRSGFSVPQSDFSIEESKNIVYLVDRK
                     PGGDTLLSNNSSSFFSAVENYLLSNKEEFFNLGGDKVQNFVSGKQSLRVTAIEVNEVW
                     KKNKGQIYYEPKSIRFYVNDSALNIIPLSEMTMMDLEINGQKLVLTLLDKKFNFTITR
                     INSQDIALRQAFVYLKALREYHWNQLTEFVKYY"
     gene            49635..>50574
                     /locus_tag="PKY09_02760"
     CDS             49635..>50574
                     /locus_tag="PKY09_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNB11805.1"
                     /translation="MATKIKFGTDGWRAIIAQDFTVENVTRVAEATAQWLKAKGSNPS
                     VVVGNDCRFAGNLFAETTAKVLCANGIHVKLAPNFVSTPMVSLGTLHYKADLGIILTA
                     SHNPPSYNGFKLKGSYGGPLLPAQVSEIEDIIKDQSIIDTNTLSLDDFAAKGLLQWVD
                     LETLYIDHVKANFDLDAIKKSGKKWAYDAMFGAGQNVMKKLFPEITLLHCDHNPGFHG
                     QAPEPIHKNLTEFSTLIKNAGDIACGLVTDGDADRIGLYDDKGNFVDSHHIILLLIHY
                     LHKYKGMNGKVCTAFSTTPKVQTLCKHYGLPIDVVKI"
BASE COUNT        15519 a         8967 c         9874 g        16114 t
ORIGIN      
        1 cccagttgcg aaaatatctg aataaacttc atggcattga tgtaagttgt atcaccactg
       61 ctgaagcctg tcatcacttc tgataaactt tttccgaaaa agatcttgac caaaggtatg
      121 tttagaactg aaatcaggcc aaaacatgtc atcacaatgc cgcaaaaaag taacattttc
      181 aataaaggat cactatgttg ccatttacct tttatcatct ttattaattt tgcacgaaag
      241 taagcatcct tacaattgtt gcagatcata ctatggtgaa aattggttcc gttaaattag
      301 gtgagtttcc attgttactt gctcctatgg aggatgtgag cgatccgcct tttcgctttg
      361 tatgcaaaca acatggtgct gatttaatgt acacagagtt tatcagttcc gaaggactta
      421 tccgtcatgc agcaaagagc acgcacaagc tggatatatt tgaatatgaa aggccaatcg
      481 gtattcagat ttttggctcc gatgtggagt caatgaaaca ggctgcaatc atagcagagc
      541 aggcacatcc cgatttaatt gatatcaatt acggttgtcc ggtaaaacaa gttgcatgca
      601 aaggtgcagg tgctgcattg ctgcaggata ttccaaaaat ggtgagcatg acaaaggcaa
      661 ttgttgatgc agtaaaactt cctgttacag taaaaacaag attgggatgg gatgataaaa
      721 caaaaaatat tcttgaagtt gcagaacgtc ttcaggatat agggattaaa gcgctaactg
      781 ttcatgcacg aacacgatca caaatgtata aaggcagtgc cgattggagt ctggttggag
      841 agttgaagaa caatccacga ctgacaatcc ctgtttttgg aaatggtgat gttgactcgc
      901 cacagaaagc attggaaatg aaaaatcgtt atggcgttga tggtataatg attggcagag
      961 ccagtattgg ttatccctgg atttttaatc agataaaaga atatcttgca ggtaactgcg
     1021 taattgccgt acccggtatt gccgaaagag tagaggtgtg ccggacacat ttgataaagt
     1081 ccgttgagtg gaaaggccct attgtaggga ttcttgagat gagaaggcac tataccaact
     1141 attttaaagg aatggatcac tttcgtgagt ttcgcgttaa gttagttacc tccaatagcc
     1201 ttgatgagtt gcttgaaatt cttgagcaga ttaaagaaaa gtatgaatta gcagcatgat
     1261 taaaacgcag caacccatcg gagtttttga ctccggaata ggtggactca cagtagctgc
     1321 tgccatcagc aggttgttgc ctcatgagca gctggtatat tttggagata ctgcccatgt
     1381 tccttatggt gacaaatcgg ttgagttaat tggcagctat gcagaaagaa ttacggagtt
     1441 tttgttggac gaacaaaagt gtaaagctat agtcatagcc tgtaacacag catcggctgc
     1501 agcctattcc ttattgcgcg atcgctataa aggaagtgtt ccggttatca acgtcattga
     1561 cccgatgatt gaggcagtta ttgctgatga tgatataaaa agtgtaggaa tcattgcaac
     1621 caaaacaacg attgcatcag gtatttatca ggaaaaattt tcaagacgta aaccgcagct
     1681 gctttttcat ccattagcta caccattact cgcttcaatg attgaagaag gattttataa
     1741 tgataatatt tcttctgctg tattggaaga gtatctctcg catgagtcat ttaaatctat
     1801 tgacggattg gtacttgcat gcacacatta cccgctcatt aaaaaggaga tagatcattt
     1861 ttttaaatca aaggttaagc tttttgactc tgctgaagta gttgcttcaa agttgaaaaa
     1921 aatactcgaa gcagaatctt tagtaaattc tgaacgaaaa gattccaata gattttatgt
     1981 ttcagatttt acagaatcgt tcgaacatag cactaaaata ttttttggaa aacaggtaaa
     2041 actggaattg atgaaccttt ggaaatagta ttttcagcaa atgaaaaaat ttacaatagc
     2101 aattcatggt ggtgcaggca ctatcagccc ctcgaccatg acgccacaaa aagagactgc
     2161 atacaagcaa gcattgcaag ctgcattaac agcaggatac aaggtgcttg aagcaaaggg
     2221 cagtgctttg gaggcagtta ctcatgcggt aattgaattg gagaacactc ctttgtttaa
     2281 tgccggcaga ggtagtgttt ttacctatga tggcaagcat gaaatggatg catccgtaat
     2341 gaatggtaaa aatggacttg ccggtgcagt ggcaggagtt cgaaatgtga aaaatccggt
     2401 tctactcgcc aatgccgtga tggaacatac tgactttgtt tttttacatg gcagtggtgc
     2461 tgaagaactg gcaagagtac aacagcttac attttgcgat gaccaatatt tttttgatga
     2521 cttccgatat cagcaatggc aggaagtgaa agggtcagat acaacaaaac ttgatcacag
     2581 caatgaaaag aaattcggga cagtaggtgc tgttgcaaaa gatattgaag gcaatcttgc
     2641 tgcagccact tctacaggcg gtatgactaa caagcgatat tcacgcatcg gtgatactcc
     2701 ggttattggt gccggtactt atgctaataa cagcacctgt gcagtaagtt gtaccggtca
     2761 tggagagtat tttattcgtt cagttgtggc atacgatatt cattgcctga tggaatataa
     2821 tgggctttcg ctaaaagaag cttgtgagaa agtggtactt gataagttag tgaaatccgg
     2881 aggagaagga ggtataattg caatagacca aaccggagta ccacaattaa tttttaattc
     2941 tgagggaatg tatcgcggtt attattgctg caatgaacaa ccggtaacgg aaatatatcg
     3001 ttgatgttta tgattcggtt tttattgttg ttcatgtttt taggtaatgt ttgtttatca
     3061 caaaaaacta cagaatatta tttgcacaac cgtacaccac agtggcatga agtaattgaa
     3121 tattataaga aattagatat gcagtttcct caggccaaac tttttactga aggaactacc
     3181 gatgtcggta aaccattgca tttgtttgta atttctgctg atggcaactt taatcctgtg
     3241 tcactacatc aaaaaagaag gttgatatgg ttaattaata atggtattca tcccggtgag
     3301 cctgatggta ttgatgcatc aatgattttt gctaaaaatg tgttgagtaa gattaattct
     3361 gtagcgtata agaatgttgt tatatgtata attccaattt ataacattga tggtgcactg
     3421 aacagaggtt gttgtagtcg tgtcaatcag gaaggtcctt tagagtatgg attcagaggt
     3481 aattatcaga atttggactt aaatcgtgat tttgtgaagt gcgatacaaa aaatacagaa
     3541 tcgtttatcc gtatttttca gaagtggaac cctgatttgt ttttagatat gcatgtaagt
     3601 aatggtgccg attatcctta taacattaca ttaatagcta cacagaaaga taaattaact
     3661 ccgcctttag gaaaatatct ctcagaaaaa atgcttccgg aactttatcg caacatggaa
     3721 agtaattctg agaaaatgat tccttatgtt aatacaatta aagatacccc cgattcaggc
     3781 ttagaaggtt ttttagagac accacgttat gccagtggtt atactgcatt atttaatacg
     3841 attggattta tttctgaaac acacatgcta aagccatatc ctgtcagagt aaaggcaact
     3901 attacattta tggaatcact tcggcaatat gcagcccgta atgcagtaga aattttacag
     3961 aaacgtgctg aggcaaatag ttatttaaaa accatacatg aatttcctgt ccaatggcag
     4021 cttgatacaa cttcaaaaga cagtatccgt tttgacggct ataccgctgt ttacagtaaa
     4081 agtaaaatca caggacttca aaggcttact tacgacagaa gtaatcctta ttctaagttt
     4141 attccttatc aaaataattt caaaccgcaa gtaataatta aacggcctgt tgcctacatt
     4201 attccgcagt gttggagtcg tgtgattgaa aggttgaagt tgaatggtgt aaaaatacaa
     4261 actataacag cagatactat catagaagtt gagtgttata aaattggtca atataaaact
     4321 acttcaaaac cttatgaagg acattatctt cattcaaatg tcgcagtgga aaagcagatt
     4381 cgtaaagtgc agttttataa aggtgacaaa atgattgaag ttaatcaacc tgctaatcgt
     4441 tatattattg aaaccttaga accgcagggc acagatgcat tttttgcctg gggatttttt
     4501 gatggtatct tgcaacaaaa agagtggttt agcgattatg tttttgaaga cattgccgaa
     4561 aagttgattc aggaaaatcc agtattgttg gatagcttga aagatgctgt taaatcggat
     4621 tctactttac agaacaatca ctacatgcag ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nntcaatttt tttatctatc agataataat agctgccaag atatttataa
     4801 gattccacca aaccattttt gtatttgtcg gcattggcag agtctgctaa tgctacttca
     4861 atatatttct cgtaataagg cttggccatt ccacctttgg agtcggggtc gagttgtgca
     4921 ttggtttggg cacgccataa tattccattg ggccatgtag gcaatagctc aattactttt
     4981 gcaaaaagcg tgtcggcatg ggtataatct ttattgaaat aatatgctct tgcaaggtta
     5041 aaatagtctg ttgatgtcag ccctttgccg ttgtttaccc ttctctcata agcattgaca
     5101 gcttgtggat attttttcaa tttcatatac atactgccca tatcctgaag gatttctgtc
     5161 ttggtagtat caatctgata tgatttttca aagttttcag cagctaaaga gtcattgcct
     5221 tctttaagta atatctttcc actgtattca tagtccattg ctgtgcgttg gttttctatt
     5281 gtagtgttca tgattttttc catcagcatt tttgcttttg gaatatcatt catctcaaat
     5341 gctatataag aagaaagacg cagcatgcca atgtttgatg aatcaattga agacagttga
     5401 ttcatttccg attgagcatc tgcataatcg ccactcataa atagaaatga tgcataacgc
     5461 attcttgcca tactgttatt cttactcaac tctaaatatt ttctgtaaga ttgtttagca
     5521 tcttccaact tacgcatttt aaaatatgtt tcagcaagtt cgcgatatga gggtgcaaaa
     5581 tcaggattca gttttatggc tgattgaaat tctgctgcag caccttcata gtttgtagag
     5641 cgtttatata acacgccttt ctgaacaata gcttttactg cttttttgtc caagtccaat
     5701 gcacggttgt aattttctgc agctaatgtt ccattgtttt gttcggaata ggcatcacct
     5761 aaaagaatat ataactctgc attttttgaa tcaacggcaa tggcgttgtt aagtagttta
     5821 atagcatagc taaggtcttt attttttgtt ctgataaaac tttcagcgac ttcactttgt
     5881 accaacgatg ttttagagcc tgacattgtt aatgctttat caaaatcggc acgtgcttca
     5941 ctgttaagac ctttctctaa ttttattttt ccggttccaa ctaaatttat tggattgccg
     6001 ggttctgctt gtaaaccttt tgagtaggca atagcagcag aatctaaatt gtcactttta
     6061 aaataatttt cgccataata aaaccaattg gtaccattgg caggctcttt ctgcaataat
     6121 gatttaaaca tgtcaccggc tatttcatat tgctcattat ctgtcatcgc aatagcctct
     6181 ttaagtgttt gtgcatgtac aacctgtgcg aacatcaggc tcagaaaaaa aactgttttt
     6241 ttcattctat taaaattggt atttataatt tagttctaat taatttcctg ttcttacaat
     6301 tctaaccggc atcgtagcgg gcagcagtcc ttttaatctt acaagccgct gtccctgatc
     6361 gcctgcaaca aatgatgcaa agccggtgcc aagtccgttt cttgactggc ggttaatcaa
     6421 aaatacatct ctggttaatg gatactgttt caatgctata tatgcctgat atggttgata
     6481 ataatctgta tattctgttg cagtatcatt tttaagtgct acaactttaa tctgattcag
     6541 aaaagtcata gcagtggaat cattgctgtc gcttatccaa ctaacaccaa ttataccaat
     6601 agcattcttt ttattaataa cgtaatccac cacctctttg tttgagttga gggcataaca
     6661 attggcagga aattctccac tctgtaagag tttttctttc agaaaacgta cattacttga
     6721 accattcttg tcaaatacaa tagcaacttc acccaaatta tttttattat ctattgcact
     6781 ccagttattt gtttttcctg taatcaaaga ttcaaattgc gataaggtta gcagcgaatc
     6841 aatgttgtct ttattgataa ttaatgcaag ggcatcaatt gcaattttag ttgagcgtgc
     6901 cagcacttta tgatctttaa aatactgatg ctgttccgga gtaaggtctt tccctgaaat
     6961 aataaccttc accgaatcgt tcatcagata attaattgca tctgcttctg tcttatagct
     7021 aatttcgatt ttagcatttt tatagagtga cataaatgtt tcagcctgac ttttcatgag
     7081 aggctcgaaa ctttcatcag aaattatttt taggtttccg gaagttggtg tgtcactcaa
     7141 gtcgggttta aaagcatcac caccacagga agctaaatgt aatgcaatag ctgttgaaag
     7201 gactaagagt agaaaatttt taactttcat caagtggttc ttgttctttt ttggatgaac
     7261 ggaaacttct gtaaccccga taaattgcat aaacaactaa aattccgcct aaccattttt
     7321 gttgaggctg atcaagcaaa tcaagattat ctgcaaatat caaaaaaaaa cccatgactg
     7381 cataaccggc aatcatgagt aaattaaagt atataccaac tttgcggatc acaccgacaa
     7441 agagttattg caatttaaag tttaccggca ggttatattg aactgctaca cttcggccat
     7501 tttgtttacc gggtttccaa tcaggcattg agcgaactac acgtaacgct tcatcgtcca
     7561 agcctgcgcc tttaccgcga agaatttttg catcctttac tttgccatct ttatctacta
     7621 cgaaagtaac atacactata ccttcaatac cgttttcacg tgccatggca ggatacttaa
     7681 tattgttttg caggtatttt atcagtgcgg cttcgccacc gccaggaaac tgaggcattt
     7741 cttctacgat ggtgaaaatt ttaccttcgt taggatcggc tacaaccggt tccggtggta
     7801 attctgttgc atttggatca ccttcctgtg ttaccacacc aacattcgtt tccgaaagtt
     7861 tttcttgtgg tggtggctgc tcttcctcaa caggtttatc aactactacc ggaggtgtaa
     7921 actttattgt ttgaacaacc ggaggaggtg gtggaggagg aggtggtggt ggctcggcag
     7981 gatcaaccgg tggtggctca gataatgtaa cttcaacttc cttttgcact acattttctg
     8041 tggctttatc acccatcagt tccagaatta atggcaaact gataaatata atgagtcctg
     8101 cagcagcaat aacaaatgct atgtttatgc gcttgctata tagttggcgt attaaatatg
     8161 cgccataact tttatttctt ccgtcaaata ccagctcatt acgtttggca aatgtcggat
     8221 cgtcccaatt gttaaaaaaa cttgttgcca tgattagttc attttttgaa ttaaatcata
     8281 ttcatccttc atcatatcta ctactgcata tttaccaaca ctggcaatgc tcagttcgtc
     8341 taatatgtca atcacatttc tgtattttgc tttatcatca gtcttaatca gaaccattaa
     8401 agcacttttt tgtgatttgg cttctactgc aagacgttta aatgttgagt cagcaatttg
     8461 tcgttttgcc aattcatctt caagacgctt aacttctgca tgtgcagctg cgtttttatc
     8521 taacagtatt ttacgaattc cattgtctga aaaatcagtt tggttaagtt gtgtttcgtc
     8581 cggtttaaat tctccgaaat accaaaacac tttgtcttta tcactcagta gtacggtaat
     8641 agcattattc acttttgttg gttcaccatc ttttaacggc atattaattt caaccgtttt
     8701 tggtttgctg aaagtggtag taagcataaa aaaggtgagt agcagaaacg ccagatccac
     8761 catcggtgtc atgtcaatgt gagtggaggc tttcttggcg cggaccttac tatgtttccc
     8821 gcctttctct ttaccaccac cctggtcggt atttacttca gccatttctt cttattcttg
     8881 tactggtgtt gcttgtaaat ttgttatcaa gttaaaacgg ttgattttgt tttgttggaa
     8941 aatctcaatc acacgtttta tgtaagtata atcaactctg ccgtcacctt ttatggcaaa
     9001 acgcagttca ttgcctgctt gtttacgggc ttcataacct tcgtcagtaa tagctgctgt
     9061 ccagccaaaa actatccagt cggcaagttg attgttgagc gtatcaattg gaataccacc
     9121 ggtagcctta tccatcttct tgcgttccga atcaggtgct tcaagatagg ttttgatttt
     9181 atcggcaggc attccaaatg ttgtcatcag agagaatttt ttcaaatcag cttctgtaag
     9241 ttggaacttg tatttctcac tgattcttgt catcaggttt ctgcgcacat tttgtccttc
     9301 gatattaaaa aacacgcgac cagctgaatc aattgttacc tgtaaagtat tttcaggtaa
     9361 aatttttgtt gaaacagatg ccggtgtgtc aacaatgacc ggttcgtccg gacggaattt
     9421 ggtagttaac ataaagaatg taaccagcaa aaacgccaga tccaccatgg gtgtcatgtc
     9481 taacgatgga ctctttttgg gtattttaat ttttggcatt atctaatgct gataattaat
     9541 tacgctaatt tatttagtgt ttgcagtaaa cgattgaaca atgctgtagc ttgcctcatc
     9601 tattccataa gtcaacgaat caatcttatt ggtgaaatag ttgtacatga taattgctaa
     9661 agcagatgtt cctactccaa gtgctgtatt aatcaaagct tcagaaatac cggttgcaag
     9721 tgctatggca tcaggtgcac cggcttgcgc aagtgcagca aacgccctga tcataccgat
     9781 aaccgttcct aacagtccca tcagtgtagc aacagatgct actgtagaga tgataaccag
     9841 attttttgaa agcattggca gttcaagtgt tgtagcttca tccaactctt tttggatagc
     9901 tgctgttttg cgttctttat ctaaagctgt ttcgccctgc attttgcgat aagattcaat
     9961 acctgctttt actacattgg caatagaacc tttttgtttg tcgcattctg aaatggctgt
    10021 attcaaatcg cctttgctta gtaaacttct gattttacct acgaaagagt caactgatga
    10081 ttttcctttt gaagcgccaa tagtcaacat ccgctcaata gaaatggtga ttacaatcaa
    10141 taataagcta atcagaattg gcacaattac acctccttta tgaataattc ccagataatt
    10201 gtttggatga ttattggggt cattttccat aaagtttgcc ggattaccta atatataagc
    10261 ataaattagg atggcaacca agattgctgc cggaattact attgctgcaa agatggattt
    10321 gaaaccaccg cctgaattgt tagatgaact catgttcgtt tttaatttaa gttttaaatt
    10381 ttaaaattga ttatctataa ttaattattt gtcaaatggg acggcaagta taatgaaaac
    10441 ttgacaattt gccgctttaa tttttttaat tcatgaatat tgagattggt tgtattgctt
    10501 tttcagaagt cataaaatta cttttcgggc ataattatcg caagtatgat gtataacaac
    10561 acacctgttc caaatccaaa aactgcaata ataaaaagca aacgaattaa tgtggggtcg
    10621 gcttcaaaat aattggcaat gccactgcaa acaccgaata gttttttctc tcctttaact
    10681 aagcgtttag attcgttcat gctgttggtt cagacagata atgattgttg ttctcgcggt
    10741 caaaaataaa taaattgttt aatttgcaaa ttcattttaa tcatctttat gaataactcc
    10801 atatttttta cattattttt atgttttggc gtttatttta aaattttttt atgagagcga
    10861 taacttttat tttggcagtt gtagtactga cttctagtgg ctgtcttaac ctcaagatgc
    10921 ctcaggttac cggcataagt aagttaagat taagcgatat aactaaagat acacgaatac
    10981 agtttgatgt aggcttacgt aatcctaatg catttggtgt aactttaaaa tcaatgaaag
    11041 cagaattgtt tcttgcagac agttctatag caggaatagg cgttgataga aaaacaaggt
    11101 tagctgccaa tcagcatgtt gacctgcctt tttctgttca gcctaaactg acagcattac
    11161 ctcaattagg tattttaggc ataacacaac tatttaagaa agacaataaa aaaatcagcc
    11221 tcagaggaga attaaaggtc agaaagttta tttttaccaa aaaggttaag ttttccattc
    11281 cttagtttca ttacgctctt gatctcagta attcctattg accagttttt aaataatcat
    11341 gcatcgcatg tagcgctgct tgacacacgc agtcctgcgg agtatgaaca tgcacatttc
    11401 ccgggagcgg tttcatttcc attaatgacc aacgagcaac gggcaataat tggtaccatt
    11461 tataaacaag aaggtaaaga tgctgctgtc ttaaaaggct tcgagttgat tggaccgcag
    11521 tttgccgaat ttgttcgtca ggcaaacaca ttagtttctg ataaaaatgt tgctttatat
    11581 tgctggcgtg gtggactacg tagctcggtt atggcatggg tattatccac tgcagggttt
    11641 aaagttacta ttcttaaagg ggggtataaa gcattccgtc aatgggttct ttcgcagttt
    11701 ttaattaaaa aaccaatgat tgttgttggt ggcaaaacag gtgcaggtaa aacagaaatg
    11761 ttatatcatc tgcaaagcga aggtcaattt ataatagatc ttgaaaaatt agcccatcat
    11821 aaaggatcgg catttggcgc attaggaatg tctgctcaac cttcatcaga acattttgaa
    11881 aaccaactgg cattacaatg gcatcaggca ccattcagtc agtttacatg gattgaaaat
    11941 gaaagtatta aaattggaaa atgtgctttg ccggttaatt tttttgatca aatgcgagag
    12001 gcaaaatgtt ttgaaatgga tgtgagctta gatgttagga tagaacgtac gcttgaaagt
    12061 tatggtaaat ttgatgttga tttattgtct gacagtacaa tgaaaattaa aaaaagattg
    12121 ggcgggctaa ggctgaaaat ggcattagag gcacttgagc aaaaagattt aaaattgtgg
    12181 tgtagcattg ttatggaata ttatgataaa acctatcaac atagtcataa taagcgtcaa
    12241 gaaaatgaaa ttgttaaagt agatgctgaa ggtatggata aatcaacgat tgcaaaagaa
    12301 atgattaaac tttcaaaaca ggttgtttta tgagtgaaat agtgaaactc acacagttta
    12361 gccggcaggg tggttgtgga tgtaaaatag cacctgaaaa attatcagag atcattcatg
    12421 ataaggagtc agttgtcttt aaacaattat tagttggcaa tgcagaatcg gacgatgctg
    12481 ccgtttttga tttaggtaat ggtacatctg ttatcagcac tgttgatttt tttatgccta
    12541 ttgttgatga ccctgttctt tttggatcta ttgcagcagc caatgctatt agcgatgtct
    12601 atgccatggg tggaactcct gttatggcag ttgccattct tggatttcca ataaataaaa
    12661 tacccttgtc tgttgctaaa gaaataaaag ccggtgcaga aaaagtttgt catgatgctg
    12721 gtatccctct tgcaggcgga cacagtgtag atagccttga gcctatgttt ggattggccg
    12781 ttaccggcat catcccaaca gaaaatgtta aacgaaattc aactgcaaga gaaggtgatt
    12841 ttttactgct cacaaaaccc ataggtgcag gtgtcataac agctgcttta aagcgaggtg
    12901 tcattagcga aggtcatctt gagcaagcga tgaaaaatat gacaacagta aatgccatag
    12961 gaaaagaatt aggcaggttg aagtgcgtaa atgctatgac agacgttaca ggttttggac
    13021 ttgcaggaca tcttaaagag atgtgtacac ccggcaactt atctgttgaa atatattttg
    13081 acaaattgcc agtgatggat ggtgtaagag aatacatttc aaaattcatt taccctgata
    13141 tgacaacaaa agtttattcc tctattgcct cacagtgtaa tgaacttaac tcagagcaac
    13201 tttttatttc atgcgatccg caaaccaatg gtggtttact cgtggctgtc actcccgatg
    13261 gacttaatga agttttatca atctttgata catacaatat tccatacatt actcctatag
    13321 gcaggttttt gtcacgacag gaaaagatga taaatataat atagcgtact tacaattatt
    13381 gaactaatct actgaatgaa attttatttt ttagtaattc aaaactggaa aaagacataa
    13441 aacttcaatt tatattatat tcgtggcagt gattttttaa agttttaata aaatgtatcc
    13501 atatcagata acatcagaag agcaatatac caatgactat cgcaacagcg ttgaaaaccc
    13561 tgaaaaattc tgggctgaca ttgcacagta tttttcctgg aagaaaacat gggataaagt
    13621 attaagtgct gattttgaaa aagcagaagt gaaatggttt gcgggagcaa aactcaacat
    13681 tactgaaaac tgtctggaca gacatattga aaaattagca tcacaacctg ccattatctg
    13741 ggagcccaac gaccctaacg aacattatcg tgttttaact tacaaagagc tgctgtttaa
    13801 agtaaagcag tttgctaacg ttctaaaaaa taatggcgta aaaaaaggtg atcgcgtttg
    13861 tatctacatg ggcatgattc ctgaactggc aatagctgtc ttagcttgcg cacgaattgg
    13921 tgcaattcat tctgtgattt ttggaggttt ttctgcgcgt tctattgcag accgcttgca
    13981 ggatgctgat gcgcgtttta ttgttacctg tgatggagcc tttcgcggag caaaagatat
    14041 tccattgaaa gcaattattg acgatgcact tgaaagttgt ccgtttgtac aacgggtaat
    14101 tgtgtgtaca cgcacacata ctcctgtcag catgattaag ggcagagatg tatggtggga
    14161 agatgaaatt aaaattgttg aaactcaagg taaccctgac tgtattgccg aagtgatgga
    14221 tgctgaagac gtattgttta ttctttacac ttccggttca acaggaaagc ctaaaggagt
    14281 agtgcactca gtagccggct atatggtgtg gacaaactac acttttgtca atgtgtttca
    14341 atacacaccc ggacaaatat atttctgtac ggctgacatc ggttggatta caggacatag
    14401 ttatcttctc tatggccctc taagtgcagg agcaacaacc atgatgtttg aagggattcc
    14461 aacttttcct gatgcaggcc ggttttggga tattactcaa aaacataaag tagatatttt
    14521 atatactgca cctacagcaa ttagaagtct gatggcttat ggaacatctt atctcgaagg
    14581 aaaagatttg agttcattaa aagtgcttgg tactgttggt gagccaatca atgaagaagc
    14641 atggtattgg tactatgaga atgtgggtaa gagtaaatgt ccaattgttg atacatggtg
    14701 gcagacagaa accggtggta taatgatttc gaatcttgca ggtgtaacgc ctcataaacc
    14761 tacctatgcc acactgccaa tgccgggagt gcttccttgt ctggttgatg aaaccggtaa
    14821 tgaacttaca gataatggtg tcagtggtaa tttatgtatt aagcaggcat ggcctggcat
    14881 gattcgtacc acctatggcg atcatgagcg ttgccgtcag acatattttt cgacttataa
    14941 aaacatgtac tttacaggtg atggttgcct gcgcgacaaa gacggtaact atagaataac
    15001 agggcgtgtt gatgatgtac ttaatgtaag tggtcaccgt atcggcactg ccgaagtaga
    15061 gaatgcaatt aatatgcatc agggagttgt tgaaagtgct gtagtaggtt ttccgcatga
    15121 tataaagggg caaggtattt atgcctttgt tatttttaat ggaatgcatg ttgacgagga
    15181 acacacaaga aaggatgtaa ttcagacagt gtcacgaatt attggtgcca ttgccaagcc
    15241 ggataagatt cagtttgtgt caggacttcc taaaacgcgc agcggaaaga taatgcgaag
    15301 aattttacgc aaaattgcag aaggagaatt aaataacctt ggcgacacta caacacttct
    15361 tgatcccggc attgttgaag aaattaaatc agggcgattg taaactaagc ttgttgtcaa
    15421 ttaacattat cttgtaaata acattaaacg tttcaaaggt ttccccgagc ggtgatgcta
    15481 tagttttgac tcacaagttt tacatgtgaa cagggttgta gtttcttcca taatttctat
    15541 tgtcattatt gggttgatgg ctgtgggcta ttattatttt tctgcctatc gtgaaaaatt
    15601 ttctgctaca gaagatgcat tgccaaatga tgctgctata gtaataagag gtagtattgg
    15661 tgatttatat acttctttag aaaaagttgg agtgtggaat gaaaaagact cacttactgt
    15721 gatgtatcgt tttaaaaagg agatgcatac attgcttagt aacacaacca gtatgtttga
    15781 cgtagccgat ttgcttgaat cgccacagac aacaattgca gttttagcaa caggagcacg
    15841 caatgccgaa ctactttatc ttgttccttc acgcaaagag ttaaatattg atgctttaca
    15901 aaaagcgcta acaggtcaga tgagtaaaaa taaatcaaga acatttgccg gtgtcagcat
    15961 ttacgaaata caattcagta attttaagtc atcatttacg tttgcttttg ctaatggtat
    16021 ttttattgga agctttaacg caggcttggt tgaagatgct ataagacaac aggcaacagg
    16081 caaacccttt ggtggtaatg tttcagcaca aaagctttac aaagaagtgt ctgtttcaac
    16141 aggggccata ctcggcctta attatcctgc attaaaaaaa cttttcagtg ttgcaataag
    16201 ttcagatcag tttaatgaat tagctaatct tgaaaaatgg gcagaatggt cttttcacaa
    16261 cctgacattt gatggaaatt ctattaatgc aagcggcaca gttcttattt cggattcaac
    16321 actacaaatt agtgattttg gaaatgcacg ttcctcagca gatttgttgt ggtcagtact
    16381 tcctgatgat gtttctgcct tacagattat ctcctttaaa gatgctcaaa tttggagtga
    16441 taagaataaa agaagaaatg cagataatga agaaatcatc aattttatca agcaagtttc
    16501 tgcaacagaa acaaaattta aagtggaagt aaaaaaggat tttgcaacaa tagttggagg
    16561 tataggtgct gttgctgtta agggtaatcc aacttcattg cttgaaaata attcaattgc
    16621 tttattacaa ttgaaggatc aaaagaagtc attgactcaa ttattgtctt taagtaaaaa
    16681 actttcaaaa aataaggaag tcaatgctgt agaaaaattt cgtaatcatg aaatacgctt
    16741 tttgaattgt gatggtcttc tgccggcaat gtttggaaat gcttttactg ttatcagaaa
    16801 aaattatttt acaacgcata acgggtttat aatatttgcc aataaaccaa gtcagttgcg
    16861 cgggtttatt gatgatgttg aagataaaga aatgtttaat aagagtgaag tatatcagca
    16921 gaataaggca gttttgtcag aaggcatgtc ttatgcatta ttcttaagac cgactaatct
    16981 caattcatat tttcaatctg ttgcaaatag tagcacactt aaagcattgg cggcaacaga
    17041 cctgatgtat aagcttagta gtttttatta ttacatacaa ccaggtgttt ccggaaaggc
    17101 agaatgtaat tttagagttg ttatacagca aaagcaagaa caaaaagtat ctgaacccaa
    17161 atggtctttc ttcgctgatt ctgcaattca atgtggtcca tttattttta atagcgacaa
    17221 tggaatatca atcctgtttc aggattcaac aaaaagttta atttgcttag atgaaagcgg
    17281 aaatgtaagt tggaaaacaa ctatcaatgg atttattaac ggaaagattt ctcagactgt
    17341 taatgaccag ttgggattat tgcaacttgt ttgtagtaca gatgacagca tatatatgtt
    17401 taatcaaaat ggtgttttgc ctgcacgata tccgtttaag ctgcctgttc aatcgcgcaa
    17461 tggtgttgtg gcttcaccta atgccaatgt tctgtttgta gcaggtgaga ataatttaat
    17521 gtatgtattt gatcccggca gcaggccttc acaaaattgg aatacagttc aacttaacgg
    17581 taacttattt aatgcatcaa ttgatgcatc aggtaggttt gtttacataa tggatggcga
    17641 taagctaagg ctatattcaa cagatggcaa gctgatttta tctatgccat cagcagagta
    17701 taaagtaaat tcaatacaat gggttaatga ctctgtgcca tcactcttta tgctgaataa
    17761 taaaggaaac attgttgttt ggaatacaga tggcacatat aaaattgcgt caggtgatag
    17821 tgcttttgat atggcagcaa catgtggtac aggaacagca agaatgtggt acactattaa
    17881 aggtaaggag ttgaagattt tggattacac cttcaaagct attcagaacg cagatttaag
    17941 taagattgta tttaacgcca tgcagctttt tgatacaggc aatgaaattt tatgtatcct
    18001 taaagatgct gaaaacattg caatttacag aggtgatttg aaaatattgc cacaggtaat
    18061 tcccgcagta agttttgctg ttaaaacatt tcagggaaaa acctatttga tttctcgtac
    18121 agggaatggc aaacttatct tgtatgtgct ttagttattt tgtttggtaa acttgtaata
    18181 ctgatagtca ataaaatcat ttactttaac agtgatgttg tttaccttcc cattaacaac
    18241 ttcaatagga aaagggtgaa tgccataagt aggatcgtta aaagtgcaca gcaatgattt
    18301 ttcagagagt ggttttagcg cgccacgcaa ttttgggtga tgcgaaaaat acatcataag
    18361 tcctgatttt tctattttga tttctatttc accatatacc tcattgaagt attttccaac
    18421 caatgcagca tctgcaacac taagatgcac ttctgcattt gcttgctttt tcaaattctg
    18481 aatttcttcc gactcagtaa tattgttttt tgaataaatg ccaaagtagt tttcgctaac
    18541 atttgtgtag gcttgttttg caaaataatt tatcagttgt tggcgcaatg ctccataaaa
    18601 ccaattagca tctgtattag tcagaataac aaaaccggca ttcagttcag gtaacaaagt
    18661 ggtgttggtt acaaagccgt tggctccgcc atcatgagat acaattttat aaccatacat
    18721 atcttctgac ttccaaccaa gtccgtaatt ttgaaagtga ttgtaaggca tacgtgcaga
    18781 gcctgattct ctaacaatca tataagaatt ttgagtagtg ttaatcactg cagatggaat
    18841 tacttctttg ttttcatatt ttcctttatt taattgcatc attatccaat gtgagatatc
    18901 gttaacgcat gagttaattg atgcgcatgc accaatgttg tcaatgtttg cataatctaa
    18961 taaaactatt ttgttgtcaa caagtgtata tggttttgct gcattgacat cagttacaat
    19021 tttttgatga taaacactgc tccgattcat ctgtaaaggt ttaaaaaaat gatagtcaaa
    19081 aaaatcttgc catgaggtat cagtaactgc tttgataacc tcacctgctg ttacgtatcc
    19141 catattacaa tatccatact tgtctcgaaa ctcatttacg ggctgaacat ttctcatgtt
    19201 ttttattaat gcatctgttg tcatgttgca gtcccaattc aagaaatcac tttgaaaggt
    19261 ataaaaacca attcgatggc agaggatatc tttaatggta accatattag ttgcacatgg
    19321 gtctttaagt tgaaaccagg ggagatattt agttacttta tcatccaatg aaatccgttt
    19381 ttgattagct aagagtgcta atgcagttcc tgtgaaagcc tttgtattgc tggcaatctg
    19441 aaataaggta aactcatcta ccttgtccgg tttccctatt tccctcacgc cataaccttt
    19501 catagctagt actttcccat cttttactat ggcaatagca acaccgggaa tttgccattt
    19561 ttgcatttca cgattaatgt aactgtcaag actgtctttt ataaaattgt cttgtgaaaa
    19621 tgtttgaaag gtggttaacg atgttaacag aataagacat aagaattttc tcatttcgat
    19681 aaatttaatg tatcaaatgt atggaatttt aaataagtaa gcacctgcat tgtagtttat
    19741 ccggaatgcc ggttctactt tctgaaagca agctatgcca atgggtgtgg cttgcttatt
    19801 ttttgtagaa agatttttct aaactgtaaa tgagtctcag ataaattacg aattttaccg
    19861 ttgattatat gactcagatg tttaatacag aaaaatacaa gaaactatgt gaagagttgt
    19921 tgccctataa agccagcctt attgcagtta ccaaaacaaa accggtttca gatattgaga
    19981 ctgccattca aaatggacaa aaactatttg gcgaaaatta tgttcaggag ttggtggaga
    20041 aggtgccagt attatcgaaa aatatccaat ggcattttat cgggaatcta caacgcaaca
    20101 aagtgaagca gattgcacca tttatccaca cagttcaaac tgttgacagc aaaaaacttt
    20161 tacatgattt agataaacat gctgcagcaa ataacagagt aattaaaatc cttttgcagg
    20221 tgcatattgc aaaagaagaa acaaagtttg ggttttctgt tcctgaagtg caaatgttga
    20281 ttgatgaagg tgtatttcaa caatacaaca atattagttt taatggattg atgggaatgg
    20341 caaccaacac agatgatact tcgcaggtac gttctgagtt taaacaattg tatggattgt
    20401 ataaatcaat ttccggtgca tttgacagaa gtgaatctcc ggtattaagt atgggcatga
    20461 catccgatta taaaattgca cttgatgaag gaagtaatat gattcgaata ggttcagcgc
    20521 tttttggcag cagatagttg tatggggttg ttatttgcac ttgtcacaac actttcatgg
    20581 tctattggta tttttccatt tacagaagcc agccgtaggt tgggcatcaa ttctctaaac
    20641 catttcagat tatttcttgc tgtaatttta cttttcgtaa cagcattttt gataaatcat
    20701 tcagagttta tcaatctttt caatgcacaa tatgttcagg cttggatttg gtttggacta
    20761 tctggtgttg ttggattagc attaggcgat tatttttatt ttggtcttta tgcaataatg
    20821 ggggccagga taggaagtat cttttctaca ctttctcctg cagcagcttt agtaacatct
    20881 tatttttttg ttgatgagcg cattaatttt attggtgtta tcggaatatt tctcactatg
    20941 gcaggggtag tatttatatc agtttcaaag agtgaaagtt tttcagcaga aaaagggcgg
    21001 tacggcagtg atgttaaagg tgttttgttt ggcgtacttg cagcgttttg ccaaggggta
    21061 gggttggtgc tcgccaagaa aggttttgtt gtcttggagc aggaaggaaa gtttattcat
    21121 cctattaatg ctacatttgt aagaatggtt gtttcgctga caacattagt cttaattaca
    21181 acaattttca gaaagtggtc tgatgttttg cgaccactaa aatcaaatag tgcaggactt
    21241 aaatatgcag ctgtaggaac ttttttcgga cctgtttttg gcgtttgtat gtcgctttac
    21301 acaattacac tgcttgaagc tacggttgca caaacaattt tttcattggt gcctgttgtg
    21361 gcagcattaa ttgcccgcta tttctataaa gaaaaattta ctttagcatc agtaattgca
    21421 acattaattg ctgttgccgg tgtggtgttg ttgataaggc gtaatgaagt tcttgaatgt
    21481 atgtataaaa ttaattgagt aatgagaaga ataattttac ttgtttttgg ttttgtttgt
    21541 attgtaaaga ttgcaattgg tgaaagaccc tatttccagc aacatgtaga ttataaaatc
    21601 tctgttacgt tggatgataa atcaaatttt cttaccggat tcgaagagat gatttatcgg
    21661 aataattcac aggatacttt gcgtgagctg ttttttcact tatggcctaa tgcatacaaa
    21721 gacagaaaca ctgcattgtg taagcaatta gttagtcagg gaaatatgaa tctctatttt
    21781 gcaagtgatg agcaaagagg ttttattgat agcctggaat ttaaagttga tgacaaaggc
    21841 gttactgtcg tttatgattc cattaatatt gacatctgta aaatcgtttt aaataaacca
    21901 ttattgccta acggcactgt aataattaca acgccattca gagtaaaaat tcctgatgcc
    21961 aagttttcaa ggctaggcca tgttggacag tcttatatga tttctcaatg gtatcctaag
    22021 cccgcagttt atgacagaga tggttggcac gctatgcctt atttatcaat aggagagttt
    22081 tactcggaat tcggttcttt tcaggtggat atttctgttc cggcaaatta tgttgtagct
    22141 gcttcaggtt atttaaaaac tccttcagaa gttgagttta ttaataataa aataaaaact
    22201 gaagtcaatg aaatagattt ggataaacga aatacttttc ctgagtcaga gcgagaattc
    22261 aagacaattc gttttgtggt tgacagtgta catgactttg cctggtttgc cgacaaacga
    22321 tttcgtattg tatctaaaag tatagaactt gcttctgggc gtaaagtgga ctgccgcgca
    22381 tattttacac ctgctaattc ttatatctgg catcgtgctg ctgattatgt ttcagaagca
    22441 gttttgtttt actcaaagta tgttggcgaa tatccttatg atgtttgtac tgctgttgac
    22501 ggaactattg cagcgggagg aggaatggaa taccctacaa taacagtcat tgggaataca
    22561 ggtaatagta ttgtcttaaa tcaaagtatt gcgcatgaag tggggcataa ttggttttac
    22621 ggtttgcttg caaacaatga aagagagttg ccttggatgg atgagggtat aaattcgttt
    22681 tatgagcaat tgtatatgac acaatattat gcaaaagaaa attctgtagc atatcagttg
    22741 attggagtac catataaagc tgcaaaaatt tttggtgcag ataaaatttc attttcggga
    22801 tttaataaaa ttagttattt gctcacagcc agaacgaatg atgaccaaag cagcggactt
    22861 acaagtgagt atttctcaaa tttaaactat ggtacagata tttattccaa gatacctttg
    22921 tgtttttcgc agctcagaga atatttagga actgagaaat atgattcctg catgcatact
    22981 tattttaata tatggaagtt caaacaccct gatgaagcag caattaaaaa tgtatttgaa
    23041 actacttcgg gaaaatcgtt agattggttt tttaatggaa tgataaacag caatgcaaag
    23101 ggagatgcta aaatctgtaa agtgcatgct gatgcaggag ggactgatgt gttgttaaag
    23161 aataaaggaa atcttgccat gcctgttaat gtatcattct acaataaaga gaagttaatt
    23221 gcttcacaat ggacagaggt ttttgccggt aaattcaagt tgtcaagcac tgttgtcgga
    23281 gctgataaaa ttgtgttgga tactaatgat gaaagtttag atttaagtcc ttttaataat
    23341 tcaataaaga caaaaggtat ttttagaaaa tggaagccgt tacaacttcg ttttctgact
    23401 atgcttgaaa atcctgaaag gatacagttg ttttatctgc ctgctgttgc atataataat
    23461 tataacggat ggatgacagg cgttattctg cataattatt cattgttgaa taaaaaattt
    23521 gaatttggaa ttgcacctat gtatgcattc aaatcaaaat ctgttgcagg cattgcaacc
    23581 gcaagctatc agtttaatat ttacaaaggt tggtttgata gaatagttgc acaggtttca
    23641 ccttcgctgt ttgatgaagg attatcagaa aacaaaaaag tttttaaagg atatttttct
    23701 attcctgcct cattgagttt tagagtccgt aagtcgcagt ataaaccata ttcagaaaag
    23761 tatgtaaacg ttacccatca gtatgtacac caaactttca gtacacaaag taatttggca
    23821 gcaactaatg caatcaatat tacacaactt gagtttgcaa gttataatta taataagact
    23881 gacccgggtt catattcttt tattctcgaa aggggaatcc actatgttaa gttatatgga
    23941 acttggaata aggaaatcac actaacaaga agaaaaaagg atttgtcatt ccggtttttt
    24001 gcaggaactt ttttggataa tactggtaat ggtggtgaag ctaacttccg ttcaagtgca
    24061 tggcgaggca ctgatgatta ttcttattca tggttgtggt ttggcaggaa tgaatacgat
    24121 ggattctggg cacatcaaat gcaaaagtct gatggtgcaa tggctgcata ttatcctgtg
    24181 cggacagatc attggcttgc ggcattaaat gtttcggtta aaacaccagt acccggcttg
    24241 aggttgtttg ctgacggaaa tacattttat aatgcaaaca aagtaagtcc tggtgcaact
    24301 gtaattcgtt atgatgccgg aattatgtta agtgtcgttc atgatgcatt tgagatttat
    24361 tggccactga ttgtttctaa ggaagtaaga gattactatg acttgaacga tataaaacta
    24421 acagacagga tccggtttgt ttttaatata aacaaactga atcctgtctt gctacgcaaa
    24481 atgcgttaag agctttatct atattccgat aattttagtc tgtacggttt gtcattaaag
    24541 aatagtgtta cattatagac tcctgacgga tgtccatctg caatagcact gaacggaatg
    24601 ctgtagctac cttttgattg taagcccgca tcaatatttt tcagtattct acctgtcata
    24661 tcagtaatta gaattacaac agtgccatca gttggaatag tgtaattaat ctttgtacta
    24721 ccacgatatg gatttggtgc tgcattcaat ataacatttt ttaagtcacc tatcattgca
    24781 gtttttacaa aactatgttc aaacttaccg tcataatcta tttgcttcaa tcggtaatag
    24841 taaagaacac cttctttaac attcctgtct tcaaagctgt agttgcttac ctgattagta
    24901 gttccatgac catcaaccca ggtgacattg tcgaagtcgg tgccatcagt gcttctctct
    24961 aaatagaagc ctgcattatt tttttcactg gctgttgacc atgtcaggaa aatactttgt
    25021 ttgcccgcag ttgcttcgaa tgaaagtaat tctacaggta atggagatac tacgccttgt
    25081 gctgttccca ctaatatact ttgtccgctt gcaaaactca atcctacacc atttctcaag
    25141 actgccgtaa ccggtggtgt tacacatcca ccacttataa caggtggaat tatccaacca
    25201 gtaccatttg gattgatacc aacaccataa ttggttttgg catttgtata ggagcgattg
    25261 taaagcgtta tgttagcttc agcatttgtt gcattagctg gtgttaatgt ccaatagcca
    25321 ttgtctaaca tggtatcatt gtaattcatt aagcaaggtg catccaatcc taatccccaa
    25381 atggttgttg aatagttgga gaatgaagct tgaatacttc ctactgcagg atttattcca
    25441 ttatataaat ccaggctcag acgttgatat ccttttgttg tatttccaac agggaaatca
    25501 taaactcttg gagcacttac tgccggcaca ttgcgtctga gatagccttc aatataagaa
    25561 ccactcgaac ctgcattgac tgctgatgat gcatcattca ttacatatac ttcatggctt
    25621 gctgttgtct gaattctgga ggcaagtaag cttaatgtac ccgtagggcc aattttcata
    25681 tctgaaataa gcaccactgt actaccggcc ttatttacaa caacatttgt gagtgtatca
    25741 ttattattta tgttttgtgt tcctaatccg tttaacacaa ggtttccatt attacccata
    25801 ttaaattgtc ctgcagaatt tgataaactt cctttcaaaa taacagtctt gttaatggca
    25861 tcaaatcttg agttagggcc aattaacgcc agacttccct gcatttctat attctgaagt
    25921 aagtcaactt tccctcctgt tttacttaca gtgatatggg agaacctgtt tggcgaaata
    25981 agattaccgt tgactgtttg atctacgcta ccattattaa acaaaaatcc tgatccggta
    26041 gcaatattta attcaccatt gttctgtaca ttaccacaga ttgttagaaa ttggttgtta
    26101 tccatgatta atgttccacc cggttcgagt ttaagatctt tacatgaagc acctgctgtt
    26161 ccaatatagg gctgattggc tcctgagagc ggaatgacag catttactgt acaacttgga
    26221 atattacatc caccccagtt agcagcagta aaccagtcgt tgctttctgc acctgtccaa
    26281 taggctgtgg tgccacctgc tgtataatct accggtgccg aagcggggaa gttgagtgta
    26341 aaccctgaaa tactgttaga atagttactt actaccaaca aataaacatc tccggctgct
    26401 gcactgattc ttgagttgta tgcaccatta aatccaggat atgctgcagg tgctgtaccg
    26461 tttgaattgc tgtaaagtcc tgttaatcct aatgaactat agttacatct taatggggtt
    26521 gcaccaccgg caattccggc acatgtagta gctccggagc ctgcaatttt ccaaactgca
    26581 aagtcatagt ccgtacaggc agtgcttgga gcacctaccc aatcattagg aataatattg
    26641 aattcaagat ttccggcact ggcaatattt atctgatacc atgctgaacc tcgttcagca
    26701 gtatataagc agtttgttcc tgctcctgga aaatcacaat tgttaccaaa aagttgaaat
    26761 cccggatctc caacctgtat gttttgattg caaacaataa tcggtgagcc acaatcctgt
    26821 ccttcaatct ccggcaaagt agttccgcca ttaacggcaa taatactgaa tgttcctgtt
    26881 aaataacctt ctccatcaac gacaacataa taagtgttgc ccggtgtcag tcctgttaaa
    26941 cttagttgtg acatagtagt tggtgttggt gaggttccac cacaacccgg tgcatttaaa
    27001 ttacaggcta taaaagtcat tcctgatcca caaacccctg agtaggctgc catttgtgtt
    27061 cttcttaaag tgcccggatt tgttctgaca gtgacattac ctgatgcagg agctataaaa
    27121 ctataccaaa cagaatttac cacattcggt gtagtccaac atcccggtgc tgccggttct
    27181 ccactgccgt cagcacaatt gctattgcct gtatagtaaa gacctaattc catactttgt
    27241 gcgttacacg gtaagtcatt atatacagag cttacattag ctatgcttat ggtgtagtcg
    27301 gcattttcag ggtttgccca ggtgtcaaca attatatagt aagttcctgc tgaaggtaag
    27361 gttacagtgc cagataaaaa tccactggtg gcttgtccta aagaagcaac gcaagatgta
    27421 ccgggtgcat ccgggcatcc gttatatacc tgatagccta tatagtttgt gctggcagaa
    27481 gaaagtgtta ccgagataca acgggcagaa gctgttgtga aactgtaaac tttatcttca
    27541 ccagactcat agtttgttcc acaagaggat atactcgaat tgttataatc atcaccaaag
    27601 caggctgtag tttcattata gcgtttaaaa ggcaatgagg caattactaa tggacttgca
    27661 caagtacggc caacaacagc agttgatgga gcggaaatgg aaaggtcaaa agcaaaacaa
    27721 gaagtacgtg aatctattaa caaatagtaa gtcgttcctg ctgtaactgt tgcgcataag
    27781 gttttatcat cattgccggt ttgtgcacca atacagtttg ctgttgaagg gccacatggc
    27841 gtagcaagcg gacaaccatc ataaagaaac attccatttc tactggcggt tgttttcaat
    27901 cttatagaaa tatcacctga tgttgcaggc gtaaaaatgt aaacgttatc ttctgaacct
    27961 ttgtagttgg tagaaccaca agtgggtgta ttaccatcgg taaggttatt tccatttcca
    28021 caggttgatt gttgtgttgc tacataaggt aatgatgcaa cattaatcac acctccacct
    28081 aagttgcatg aaggcgttgt gggtgcggtt atgctaaaat tggtaaatgc aaaacaacct
    28141 gttgataaac gacaatccat gacaacataa tatgtaacac ctgcagtaat acagaaagtt
    28201 aaactcttat taccattacc ctgttgcgta cttacgcagt tggcaccatt gcctgttaaa
    28261 ggacatgcat caaatacaga aagaatagtg ttgttggcgc cagagttata agaaactgtt
    28321 atttgtcccg atgtagttgg ggtaaaagac caaatcatat cttccccatc ataataattt
    28381 gcagttccac atgcagtaag tctgatactg ttaacatcat taacttgacc gcaggtagtt
    28441 cccgaagcca tagtataagg taatgaggca atagtagtat atccggtgcc taagcctgca
    28501 gggcaactat agctaaaatc atagcgatag gctaatgtgc caagattggt tcggctatta
    28561 caaaatgtcc ttgtggttaa aacacgataa gtaccggttg taacgcaagt ccattcgaga
    28621 taggatgctg taccacaaaa gtcattgtta tatgcacccg gaatggctac tcctgcattg
    28681 gtgtttatag ttatttctgt attgtaactg gtactgccac catcggctgc acagaaagaa
    28741 aagatatatt tatagccagc agttgcagga aaggtcaaaa actcccttcc atcaactgcg
    28801 cgtgtttgcc aattaataga tggagtaata gctcctgcat ttgacccacc actgcattgt
    28861 gcatttatgc tacctgagtt tatcaggact aaagccaata gtattgtaat gtgttttatt
    28921 gttgtattca tacctattga tttatagatt tactgtaatt gatttcttct ttggcattac
    28981 aaacctgttc ggcaagacga aaggttgcct gctttttttc taaagcagaa tattgcatct
    29041 tatccataag atcataaaat tcatctttag aagctgtggg catactaacc atgagcaaat
    29101 gtttgctttc gcaaaagcca tgaaattcca gaccttttat ttgttttagg ttttgcttga
    29161 aatcagcaag ttgtttgact aaatccaact gcggaatctc aaacacaaat tcttctgatt
    29221 tctgagcaga aattttttcc tgacagtaag ctgccttgga aagaagcatt atcataactg
    29281 caaaaacaag ttttttcata ttaaaatgat tttaacagtg cttatataca cccaagttct
    29341 tcaaaaggtt atagttaggc cagcataatt acttttgtaa gaagtgtaat atttttagga
    29401 ttaatgttga cagttttaaa atcatttaaa taatttacct tgtagtttga tttttattac
    29461 agaaattagc agcaaattat ttttaaaact tgagcggcac aggtaagaaa atttatttta
    29521 taagtgactt tcatctcgga gcaccaacaa gtgaagagag tttaataaga gagcgcaaaa
    29581 tctgtcaatg gttagaaaca attaagactg atgtttcaat actttttata cttggtgatg
    29641 tatttgattt ttggtttgaa taccgccatg tagtgccaaa aggatatgta agaattttag
    29701 gaaaattggc agaaatagcc gacaatggag tgccaatcta tctttttacc ggtaaccatg
    29761 atatgtggat gtttgactat ttgccaaaag aattgggtgt taaactttac cgacagccga
    29821 aagaatttgt atttgataac aaaaagtttc taatcggtca tggtgatggt ttgggacccg
    29881 gtgatcatgg atataagttt attaaaaaag tttttgccaa taagttttgt cagtggttgt
    29941 ttgccagact ccatccaaat ttcggaattg caatagccaa cttctggagc cgtaaaagca
    30001 gacaacatac cggacatcat gacgctaagt atttaggtga tgagaaggaa tatttggttc
    30061 agtatataat cgaaactgaa aaaacaagtc attttgattt ttatattttt ggtcacagac
    30121 acctgcctat tgataaacaa ataggcgata gtcattatat taatttgggt gattggcttt
    30181 cctattttac ttatgcagaa tgggatggaa gtaaactttc tttaagaaaa tttgaagcat
    30241 aagaattttt atcatgaaaa aatttaatgc tttacaattt ggtgcatggt tctttctggc
    30301 tatttcaatt cttgcaatag ttgtgtcgtt tcgttattta aaatatgtat caacaggtgg
    30361 cgattggtta actataatat atctgactgc aactatactt agtcattttt ctgccttgtc
    30421 atttttaatt tatggcttac tgtttgttcc tgtggcatta ctttgtaaga aaaggcaagt
    30481 tctaatttgg tatgctgtag taattggatc agtttcttta ctagtactca tattagacac
    30541 ttttatctat gacctctaca ggtttcatat caatggcttt gttttggata tggtttttgg
    30601 aggagctttt tcagaaatct ttgtttttga tttggtaatg tacttaaagg tagctgcatt
    30661 tgtggttttg cttacggcaa ttgtttatct acttacacga attatttgga gtttgtttga
    30721 aagaaaaaag ctgagaggag gaaaacaaat tgcatatctg ttggcaggtc ttttaattgg
    30781 cagtcatctg cttcatgcat gggcttatgc aacttcctat cgctcaattc aacaagcatc
    30841 actttatttc ccattgtatt ttccattacg cgccaataaa cttctcagta cacttggagt
    30901 tgcagacatg aagcaaaaac aagaatggga aaatttgcaa gatggagagg ataaggcaat
    30961 tcagtatcca ttagagccgc taacatttga tactgcaact aattatccta atattttatt
    31021 tattttaatg gatgcatggc agtttgaaac ttttaatcat gaagtaacac ctaatattta
    31081 tcgtttttct caagggaaga ttttgtttaa ccgacattat agcggcagca atggtacgcg
    31141 tggtggcatc ttttctatgt tttacggaat accttcttta tattgggaag attttctgat
    31201 taacagaatc agtcctgtat ttctggatga attacagaaa agaaattacg aaatgaagat
    31261 tcttgcaagt gcagccttgg taagccctga gtttgataag actgtttttt ccaaaattaa
    31321 taacttgcgt acacacaccc ccggaaaaaa ttttacggaa cgtgatgaaa acataaccaa
    31381 agagtttatt caatggatga atacatctga ttttcaaaaa ccattttttg gttttttgtt
    31441 ttatgactca ccacacaatt acaatctgcc atactggttg cctcatccct ttaaacccta
    31501 ttgggaaaat gtagatcata cagtactcaa caatgatttt aatcctgttc cattttataa
    31561 cagttataaa aatacagttt ggtattgcga ctcattgata caaaaagtgt tgtctaaact
    31621 tgaagaaaag aaattgttgg agaatacaat tgttgtaatt acgtccgatc atgggcaaga
    31681 gtttaatgaa aacaaaaaga attattgggg gcataccagt aacttttcca agtatcaact
    31741 tcaggttcct tgtataattt catggccggg aaaaactgcg gaaacagtca gtaggtttac
    31801 atcacattat gacttagttc ctacgcttat gcatgatgta ttaaagtgca gaaatttatt
    31861 ttcagattat tcattcggta gaaatttgtt tgatacaaca gcacaacggg tttttgttgc
    31921 cggaagtaag gagcattttg catttataga tttacaaaag aatcagatta atacagttaa
    31981 ttttaatggc tcaatgactg ttacagacat gcgattgaat gaattgaatg ttaagcctga
    32041 tgcaaagatg ctgaacgatt gtgttttgaa cttgcagcga tactacaagt cacgttaaaa
    32101 tgagttttta ggtttaagga aaatttctgc cagcatacaa cgcacactgc caccgccaat
    32161 ttgttcaatt gtatcaatcg gggtgtgtaa gatgtctgaa tgtttttcaa tggtttttat
    32221 ttgttctgtt gaaaatgcat taaaggctgt atttgacatt gcaagtacag gttgcccatt
    32281 ggtggtttga agttgtaaag cattgccaca gaaagaatgc acttgctttg atgataacag
    32341 gacaggatcc aggttgttag cttcaaaata ttcaaataga gtagtgtgtt ctgattttga
    32401 atttaaatta tctgttgcta tgataacaaa gccatcacca atgcacatgc atacattggt
    32461 atgataaacc ggaatgttgt tttcatcagt ataattcagt attgttgttt gataagctaa
    32521 tttattgttg atgttagtag ccaactcaat attggttcgc tccgaaattg aaacaaagca
    32581 agttttattc ttgtgatcaa acactaagct gccagtacct tctaaaaact tattttcatc
    32641 ttcggattgt gtcaggtcta tagtctcttt aatttgaaaa gaattcttta aataattaat
    32701 aagtgaagca tttcgttctt ttcttctgtt agcggcaagc ataggataaa gcactaaagt
    32761 accattatca tgtgtgctaa accaattgtt tggaaacaat gcatcaggtg tggcactttc
    32821 gcaattttca taagcataaa tcacattgat gttgttatca atcaactttt tccttaatag
    32881 gctactttct ttttcagctt gaagctgtat ttgctgattg cttaatctgt gattttgttt
    32941 ctgaaagaca ttgtccatgc ttgtttctat gttaaagcca aacgaagttg gccttatcat
    33001 aagaacagtt gatgcaaaat gcatattgtt tagtgcttgc caactaatgc tgtcagttca
    33061 gctaaacgtg tgctgtagcc catttcatta tcgtaccagc caacaacttt taccattttc
    33121 ccaagtacag atgtgagtac tgaatcaaaa atgcaggaat gtgtattgcc gataatatca
    33181 accgatacga ttgggtcagt ggtatattca attatacctt tcattttacc ctgtgatgct
    33241 tccaaaaact tttgatttat agcttcaaca gtggcatcgt gtttaaggat acaagtgata
    33301 tctgttattg aaccatctat taccggcact ctgattcctg cgccaccaat cataccttta
    33361 aggtcaggaa aaatttcagt tacagcttta gcggcacctg ttgtggttgg aataattgat
    33421 tgtgcagcag cacgtgccct gcgtaaatct ttatgcggag catcatgtat attttgatcg
    33481 ccagtataag catgcacagt agtaatgtaa gcactttcta ctccccagtt atcctgtaat
    33541 atttttatca ttggtgctgc attgtttgtt gtacaacttg cattagaaat aatttcttcg
    33601 cttccgtcaa gaatattttc attgacaccc aatacaacag attttagatt tcctgatccc
    33661 ggtgctgaaa ttattacttt tcgtgcacct gcttttatat gtgcttttgc taaatcagct
    33721 tttgtgaatc gtcctgttga ttcaattact acgtcaacct gaagttcttt ccaaggcaac
    33781 atggcaggat ctttttcagc ataaacagga atagtttggt tgttgatgat gattgctttt
    33841 tctgatgcag aaacagttcc atcaaacttg ccatggatac tatcgtattt aaataaatgg
    33901 gctaacgttt ttggatctgt taaatcattg atgccaacaa taattaaatt tttattctgt
    33961 agtaattttt ttaaaaccgt acggccaatt cttccaaagc cgtttattgc aacatgtgtc
    34021 ataatttatt ctaattaaac atcaaaagtt aaatgtcttt ctgcatgata gctggaacgt
    34081 actaacggcc cactttcaac gtatttaaaa cccatttcaa ggcctatttg tttatattct
    34141 ttaaaaacag caggactaac aaattctgca atgggcaaat gttctttagt tggttgaagg
    34201 tattgtccta aagttaaaat atgaactccg ttttggtata agtcattcat tgtttctaaa
    34261 acctcttcgc gtgtttcacc aagaccaagc atcacacctg attttgttct tagcccaaat
    34321 gagttaatgc gcttaagtgc ttcaaggctt ctgtcgtatt ttgcctgaac acgcacttct
    34381 tttgtaagtc ttctgacagt ttccagatta tgtgacatta tctcaggtct ttcttgcatt
    34441 acacgctcaa ggttttccca tttaccagcg aagtcaggaa ctaaggtttc cattgttgtg
    34501 ccttgtgaaa tttctctgac ttttctgatg gtttctgccc agatttcaga gccaccatct
    34561 tttaaatcat cacggtcaac actggtgatt acacaatgtt ttactttcat taatttaaca
    34621 gactctgcga ttcgttgtgg ttctgtttta tcaactgcta atggttttcc tgttgctact
    34681 gcacaaaatt tgcaagaacg tgtacagatg tttccaagaa tcataaatgt tgctgtacct
    34741 gctccccaac attcgcccat atttggacaa ttgccacttt cacatatcgt gtgtagtttg
    34801 ttgttgtcaa caatgcctct aacttttctg tactcttcgc caataggtag ttttactttt
    34861 aaccattcag gcttcttata caaacgctct ttagcttttt cttccatact gcaaaattaa
    34921 acaaatcatt ggctgcgata gtttgaacag acttaacttt aaagtatatt tattactgct
    34981 aaaaatagaa aatcccgcat ttagcgggat aattttttat tgtggagaag atgggactcg
    35041 aacccacgac ctcttgcatg ccatgcaagc gctctagcca actgagctac atcccctgat
    35101 tgttggttga atgggctgca aagaaatgaa aaattaaaat ggcactgtgt gatgaagaga
    35161 aaaaaaagag gctgcccttt ggaggcagcc tcttttcgat taaactttat taaaacttat
    35221 ttagcaataa ccatcttttt aactaactga cggtcgttgt gcttcaatac atagaaataa
    35281 acaccactcg ccagattttc tgcgcttaca gtaatgttgt aggtgccggc agaaagattt
    35341 gagttaacag gtgtcataag cactttgcct gtaacatcta caatgtctag ttgcgtgctt
    35401 ccattgtctg ataggttgaa agtaattaca gaataatcac taaatggatt tggtgtattc
    35461 tgaatcatat caaacgtgtt gtttaaatct gttaaaccgg ttgtgcataa tgtaatcggg
    35521 ctcttcacct ctaagcaaac atcatctata gcccaacctt catcacgtac actaaagtca
    35581 gagttaaatc tccatctgaa gataacttgt tgttgccctg cagttggtaa acaaatatca
    35641 tgtcctacct gaccccagcc accgctgttt cctgaccatc cgggatgtac aggcggtact
    35701 ggtcctaatg cagtgatgct gtatgtgttc atccagtttg gatcatgatc atcacctaat
    35761 tgaatccagg tagcacctaa gttggtagag tattcaaatg ttccaccatc ttcatttctt
    35821 tcagtcatga aactgtgcca gaaattgatg ttgtatgtca atccaccggt taaagtaaat
    35881 acaggagaga acaatgcaga agtgtcacga ttcggatact gaccggcaag acttgtaaac
    35941 cacgaattta cagggctatg attgccacct agttttactg aagttcctaa atcccagctg
    36001 gaatttgcag aataagaagt tgaatttaat gttacccagc gataaccact ttcaaaatca
    36061 taacatttgg acgaagaacc tgcaccaaca gtagcagagt caaacacctg tacagaatag
    36121 cagatggtgt caccaattgg ttttaagtct gccgattgat ttggataata agtagaaacg
    36181 caaacattgt ggaaaccggg agaagcaatc caaccattaa caaatgtata attcatattg
    36241 gtgtttggat ttaatggtgt cgctaatgta accgtatcat tagagataat atttccatca
    36301 acacttaatg ttgcaacaaa gttgtttaca gcagaagtac catcattata aatctttgat
    36361 ttaaacggca tgctctgacc ggggaagata aatggagggc caggatttgc agttgaaaca
    36421 gctaccggag ttacagttaa tggaacagga acatttacac agaagtcatc aattgaatag
    36481 ccatttgttt gaacagaacc atctgaattg aaaatatagc ggaactgaac cattggttga
    36541 ttgttgaaga taggttcaag tttgcgcatg ctgcgaatcc agcctgtagg taaagcacat
    36601 aaggcatttc cttgagtcca tgcatattgc tgacttgagt ttaactggtt atcgttgtac
    36661 cagttgaact gacccatcgt taatgtgtcg catggcgtac cgagaattgt ccaggctcca
    36721 ttaccgatac gatattccat acgtgttccg tcccaattgc tttctgaagc aacattctgc
    36781 cagaaactga tggtagcatc tactgcatta cttaaatcaa aaattggagt ataaagttca
    36841 gagttggcat tggccccata aacactattc aggttaatat cccaacagtt tactccgcta
    36901 tatgcagagt tagttgtacc aaatgtaggt gttccgaatt gccagtcaga acccggagta
    36961 gtgctattat tttttgttga ccagccaatg tttccgtttt caaaatcatc acaataagaa
    37021 ttgttaaagt caacaacaat taccggaacg ccaactgctg cacctgctaa ggtatcatta
    37081 gccgaattac agtcacttgt taagtctgtc catgcagtaa ttgtatattg tccgcctggt
    37141 gttgtgtagg aagtcggtag aataatagtt gcattaccat taggagcaag gttacctgtc
    37201 caagcatatg gtggcagcac aggagtaccg tttatagcat aagcaatatt taaacttgta
    37261 accgcagcag taccataatt gtgaattgta acttgcaccg agtcggcact tccttgcatc
    37321 agcgtgtttc caatggatgc attataagag cctactgctg ttacaccggc atcgggtgag
    37381 cacggaatgc ttacacagaa attgtcaaga gacacaccaa caccacctgt accaaatgta
    37441 gttgcataga aaacaaatct gaaacgaata gttgttggat tcgttatcaa gagattattc
    37501 aggaagtacg ttgatttttt ccagccgcct gaattgttat tgaatgcagg attatttgta
    37561 aagtcaagat tggcagtagt gtaccagttt gaagcatttg ggtcacccat agtacctaaa
    37621 atactccagt ttgtaccatc aatagtatat tctacccaga aaccatctgt atttgctgta
    37681 ctgtaattac tgaagaatga catggtaagg ttactggcag ataatccggt tatatcataa
    37741 ataggcgtat ataaagtatc aatttcattt tgtacataac cggtattcag gttaatatcc
    37801 caggcattag ttcctgaata ggcgcctgta gttgcaccaa atgcaggagt acctaattgc
    37861 cattcattgg ctccacctgt tggtgttgaa agccaaccaa cgttaccgct ctcaaagtca
    37921 tcgcacgaag ttaaagtaag aacaggaata ccggtgctac tgatacaagt ggtatcatta
    37981 aacggtaacc cgtcattagg taatgaggta tatgcacaca ctgtaaatgg acctgacggt
    38041 atggttaaac aaggtgtaat agtaaataca gtagaagcac cgggagcaag gtttcctgtc
    38101 catgttgcag tttgtgttgc tccggtactt gatgtataag ttacgggagt agaagttatg
    38161 ggttggctac cgaagtttgt aattgttact tccaccggaa tacacaatcc ggcaggtgcc
    38221 tgaaccggag gttgagatat aatctgtaca cctgcatctt gtggaggtgg aacaaaaata
    38281 catacatcat ctattgcaaa tccgtctaat tgaacagatg catctgaggt aaatacaaaa
    38341 cggaacatca cactcgtttg gttgtctaaa acagttagct tatgcatagc tttaaaccag
    38401 cctggtgccc ctgaaattac ttgacttgag ccagtccatg cataggtctg actggagttt
    38461 aattgatcat cattatacca attttgtcct gttgggaatg tggtattgtt agtgccaagt
    38521 acagaccatg ttagaccatt atctgttgag taatccaaac gagcaccatc ccaaaagttt
    38581 tcacaatttg aatttatcca aaactcaatc tgcgcattaa atactcctat gaatgagaat
    38641 gttggagaat ataaataaga aatacaactt ccaacatatt gctggtctaa gccaacctcc
    38701 cagatattgg gtgcagagtg tgcagagccc ggccaaactg tggattgccc tctttgccag
    38761 tgtgtggaat ttgcattacc aacagagtct gtccataagt tttgtaagtc aaagttgtca
    38821 caaaatggga atgtaattcc ttgcgatgca taaaatgtta tacatccgga tgttggaaag
    38881 acagcagagt ttgcacaacc ggagttatcc aatgctgtga ttgagtaaca aatggagtca
    38941 ccatcattta cggctggaat ttgcccggta aagtcgccat ttcctaaatc agtcattgga
    39001 acacttacag gagtaccatt atttatggta taagttaatg ttgcactcat aattcctgaa
    39061 gcatcagtaa tatgcgactg aacattgtaa gggccaaggt tataaatggt tccttgcgga
    39121 tagccactag taaaagcaat gttaggagga tttaactcac aaacagcagc ctgaatacag
    39181 atatcatcaa taaaccatcc cggataacca cctgcaccac caccgttcac gtcagatagt
    39241 cggaaacgca ttctgaagtc agatacgcca ccccattgag agaggttaaa acgtgctaag
    39301 tgccacatac tgttggtggg cattgttccg ggtactcccg gatcccagtc caaagatgtt
    39361 gcagaggaga agtatccttg accaccaaaa gcaccataat cagctaaggt tgaatcaaat
    39421 tctgcagggc ctaatgttga ccaggtcata ccaccatcaa tagacacatc tatgttggct
    39481 gcatcaaaaa agtcaatccg gcaaatgtgc tggaatgtta cgcttacaaa gaaaaacccg
    39541 gttaaatcta tcgggtcggt ttcaagaatt gcatcagtac cgttgagtac ttgagcagag
    39601 tcgcaggcta cacccgacac actaatacgt gtgttttgaa cccaggcatt agccggagtt
    39661 gatgtaactg agtcaattgt ttccatgtct tctgagaaat aacaattcga ttgggcaaag
    39721 cctatcatcg ttgctaatcc aagacttagt gtagtaaaca gtcttttcat tactttttga
    39781 ttttaaggaa tatttgtgat tttacaagtt tcaaaagtat aaaaatactc tgacatgcaa
    39841 attcacaatt aagttttttt attaattgtt aaaatatttt gcaaaataga taaaactttg
    39901 tttctttaca tcacaattca ttgggggatg aagcgattag atgataattt tatctggaaa
    39961 gacttgagct atcttcagga gttgggatta agcataatcc tgattgcgat ttttatctat
    40021 tttttagtca gtaactacaa cggctgattt cataagttca atttttttct tcaacaacaa
    40081 ggcatcattt tgatgtttaa gtttattttt gtgaaaattt tcatatgaac attcacgaat
    40141 atcagggtaa atccatttta aaatcttttg gtgtagccat tcaggaaggc attgtagcag
    40201 aaacagccga tcaggcagta gccgcagcac aggagttaca aaagcaaaca ggaactaact
    40261 tttgggtagt taaagctcag atacatgcag gtggccgtgg caaaggcgga ggtgtaaaac
    40321 tggctaagtc aatagatgaa gtaaaagaaa aagccaatgc cattatcggc atgcagttgg
    40381 tgacaccaca aaccggtcca caaggtaaaa aagtacataa ggtgctgatt gcacaagatg
    40441 tttattatcc cggtgaagcc aaaaccgctg agttttatat gagtgttttg ttaaatcgtc
    40501 aggcaggtaa aaatattatc atgtattcca ctgaaggagg tatggatatt gaagaggtgg
    40561 ctgcaaaaac acctgagaaa atttttaaag aagaaattga tcctgctgta ggacttcagc
    40621 ctttccaggc aagaaaaatt gcattcaacc ttggtttaag tggaaatgct tataaagaaa
    40681 tggttcgttt tgtttcagca ttatataagg cgtatgaaag ttgcgatgca tcactgtttg
    40741 aaataaatcc tgtattaaaa acatctgaca ataagattat tgctgtagat gctaaagtta
    40801 atctcgatga aaatgcgcta ttccgtcatg ctgactatgc tgccatgcgc gataagtcag
    40861 aagaagaccc gacagaagtt gaagccagcg agcatgatct gaattatgtg aagttggatg
    40921 gtaatgttgg atgtatggtg aatggtgctg gtcttgccat ggcaactatg gacatcatta
    40981 aattagcagg tggcgaaccg gcaaactttc tcgacattgg aggaacagct aatgctgccc
    41041 gagtagagca ggcatttcgt atcattctga aagacccaaa cgtaaaagca atattggtaa
    41101 atatttttgg gggtattgta agatgcgaca gagtagcaca gggaattgtt gatgcatata
    41161 ataatattgg acaaattcct gttccgatta ttgttcgttt gcaaggtacc aatgccgaag
    41221 aagcaaagac catcatagat aagtcgggac taaaagttta ttctgccatt ttgttgaaag
    41281 aagctgcgca attagtaaaa gaaaagcttg gttaaaaaat gaaagcccta tcaaccaaac
    41341 ataatttttt aggtattgaa gatgaaaagt attacaacta tacagcttca aaatatgtca
    41401 ttcagcaagt accttatgaa catacaagct cctacctggc aggctccgac aaagggccgg
    41461 cagctattgt aaaagcttcg cagtatgtgg aactgtatga cgaagagttg gatgcagaaa
    41521 catataaaac ttgcggtatt gcaacgctta aagctctcga ttttaaaaat aagacaaata
    41581 aaaaggcagt tgactatatc gaaaaagaaa cgctggcctt actgaatgat aaaaaatttg
    41641 tggtttctct tggcgcagag catacggtaa ctttaggctt tgttaaagca catattcaga
    41701 agtacaaaaa tgtttcagtt cttcagattg atgcacatag cgatttgcgt gaatcttatc
    41761 acaacaacat ttattctcat gccagtgtta tgaaaagaat ttatgactta ggtttaaata
    41821 ttgttcaggc aggcataaga gcacagtgta ttgaagaagc acaattaata aagaagtcaa
    41881 agcgactaca tacttattat gcacataaca tcagaaaaga tgccaagtgg attgataaag
    41941 cagtaaaaga tttgagtgaa aatgtttatg tgaccattga tgcagatggc tttgatccat
    42001 ctgtaatacc ttctgttgga acagcagagc ccaatggcct tttttggaat gaaacattgg
    42061 cattacttaa aaaaactgcc aaagagaaaa acattatcgg cttcgatatt gttgaatgtg
    42121 cacccaccaa aggaaatatt ttatcagaat atacattagc aaaacttgcc tacagaataa
    42181 taggctatat agaagcagca aggaaagagt aggtgcttaa aacaatagtg tatggagaac
    42241 aggaaaatat caaacaaggg ttctgccaca ttatttcaga accgtttact tgaaagactg
    42301 acacgtacaa ctttctattt tccggtaatt ttatttttgg ttgtcggaat agtaactgta
    42361 ctatattgtt atttaagttt aaagataaat atactcagca ttgttgcact ttatcttgcc
    42421 ggcttatttg catttacact tgttgaatat ctgatacatc ggtttatttt tcatttcaaa
    42481 gccaacacac cgggtcagga attgctgaag tataagatcc acggtgtgca tcatcactac
    42541 ccaaaagata aagatcgttt agctatgcca ccatttatgt cgcttacact ggcagctttg
    42601 ttttttttat tgtacaaatg gttatttgga aattatggta ttgctctgtt ctccggattt
    42661 gtaagtggct attcagttta tcttatcatt cattatgcag tgcatcgtta caggcagcct
    42721 cataattttt tgagcatact atggaagcat cacagcctgc accactaccg ttcagacaat
    42781 tctgcattca gtgtatcaaa cccattgtgg gattatgttt ttggcactat gccagataaa
    42841 aaaaccattc agaaaggcga taaagaaaaa ctccctgatt attttactta atactttact
    42901 gaaaatattg tttggcttct tgtttaacta aaaatttaat caagtttcaa tacagccata
    42961 aaagcacttt gtggtatttc aacactgcct acctgtcgca ttctcttctt gccttctttc
    43021 tgtttttcca aaagttttcg ctttcgcgaa atgtcaccac cataacattt tgcagttaca
    43081 tctttacgaa tggcttttac agtttcgcga gctataattt tagcaccgat acttgcctga
    43141 ataattattt cgaattgctg acgtgtaatc agctctttta atttttcgca aatcttttta
    43201 ccaaatgtat aagaatgatc acgatggata agtgccgata atgcatcaac aggttcgcta
    43261 ttgagtctga tgtcgagctt taccaaatcg cttgttttgt agcctattgg atgatagtcg
    43321 aatgaagcat atcctttgga aaccgatttc aacttgtcat aaaaatcaaa aacaatttct
    43381 gccaatggca tttcaaatat cagttccaca cggtctgttg taaggtagtt ttggcctata
    43441 attacaccac gtttgttgat acataaagtc atgatggcac caatatattc tgactttgta
    43501 ataatctgtg ctttaatata tggctcttca atgtgatcca ttttgcttgg atccggaaag
    43561 tcacttgggt tgtgaatttc atacacatca ccattagtaa gatacgatac atacgaaacg
    43621 ttagggacgg ttgtaatcac tgtcatgtta aactcgcgct ccaatctttc ctgaataatt
    43681 tccatgtgta gcattcctaa aaagccgcaa cggaatccga atcctaatgc tgctgacgat
    43741 tcgggctcaa atactaatga agcatcgttc aactgaagtt tctccattga tgaacgtagc
    43801 tcttcatagt cttcagtatc tacaggataa attccggcaa ataccattgg tttaacatct
    43861 tcaaaccccg aaattgcttc agcacaaggg ttttctaaag tagtgatggt gtcacccact
    43921 tttacttctt tagcatcttt aatgcctgta atgatatagc caacattacc tgttccgata
    43981 gtttttgcag gtatcttgtc aagtttcaga ataccaattt cttcagcaat ataattactg
    44041 ttggtagcaa caaattttac tttgtcaccc gatttcattt cgccttcaac aactttaaag
    44101 taagcaataa tacctctgaa agaattatat acagaatcaa atattaatgc tttaagcgga
    44161 gcttttggat tcccttttgg cgcaggaata cgatgcacaa tttcacgtaa aatatcatga
    44221 acacccatgc cggtttttcc actggcagcc ataatctctt cacgtctgca tcctaacaaa
    44281 tctactatct gatctttcac caattcaggc tctgcactcg gaaggtcaat tttgtttaaa
    44341 acagggataa tagtcaggtc atgttcaagc gctaaataaa gattgctgat tgtttgtgcc
    44401 tgaatgccct gtgcagcatc aacaattaat aaagcacctt cacaggcggc aatacttctt
    44461 gatacttcat acgaaaagtc aacgtgaccg ggagtgtcta tcagattcag aacatatttt
    44521 ttcccatcta aagtataatc catttgaatg gcatggctct taatggtgat gccccgttcg
    44581 cgttccagat ccatatcatc gagcacttgt gcctgcatct gttgcttgct tatggtattg
    44641 gtatattcaa gcagacggtc tgcaagggtg cttttgccat gatcaatatg ggcaatgatg
    44701 caaaaatttc tgatattttc cattgggcgg caaaaatagc ttttttaagc cttcatacaa
    44761 ccggtttttt agggcattta cagcgttatt tgtgggacag ttattcaggt gtttttcgga
    44821 cttttgtaaa cattttttaa aagaattatt atggaagaac tgcgttatcc tattggtaag
    44881 tttcaacttc cgggaatcat cacttccgaa cagttcagta gctggaaaaa tgatattgtc
    44941 gccttaccat caaaggtgca acaattgaca gatggtttta aagcacatca ttggtcgcag
    45001 acctacagac ctgacgggtg gacagcctta cagttggtgc atcatttagc tgatagccac
    45061 atgaattgtt ttatccgttt caaatggtca ctgacagagg aaaatcctac tataaaagct
    45121 tatgatgaaa aagcatgggc taaccttcct gatgtgactc aatcttcacc gatgatttct
    45181 attcaactgc ttcatgcatt gcatcagcgc tggataaatc ttatcaataa tatgactgaa
    45241 caggattttg aaaaaacttt ttttcatccc attcaacaaa aaacacatag cctcaaaggt
    45301 gctgttggac tttatgcatg gcatggaaat catcatcttg cacatttgaa aattattgct
    45361 gataaataag tttgttaatg gcttattgct gatgattttt attttttatt tgactaaatt
    45421 ttgtttcgaa tgatttttga tttttgtttg tgtttatgag gcaaaaactc attgctttat
    45481 ccgtactctt attgatagtg ttgttgttga atttttgcac cacgcaaaaa aaacaaacgc
    45541 tgccattgct ttcatcctat gcaagtctga atgatacggt aaaatatgtg ggtaaagaaa
    45601 cttgcaggtc gtgccatgcc gaaatttaca atacgtttat gcaaacggga atgggcaaat
    45661 catttgatgt tgcttccaaa caaaaaacat cagcaaaatt tagtcatgca gttatttatg
    45721 ataagtataa aaacttctat taccatccat tttggcaaaa cgattcactg cacttgcttg
    45781 aattcagact tgaaaatggc gataccgttt ataagcgaaa tgaagtaatc aattttatta
    45841 ttggttcggg tcaacatacc aactcacata tcatcaatac caacggttat ttgaatcagg
    45901 cacctgcaac tttttatacg caaaaaggtg agtggcattt gcctcccgga tttgaagatg
    45961 gcaacaacac acgtttttca cgaatcatcg gtttggaatg tatgagttgt cataacagtt
    46021 atccggattt tgttttgggg tcagaaaata aatacaataa attagagagt ggcatcaact
    46081 gcgagcgttg tcatggtccg ggcgaatacc atgttcaaca aaaactggcc ggtattttag
    46141 ttgatactgc taagtcaata gatttttcta ttgtgaatcc tgctaaatta tctattgacc
    46201 tgcagtttga tgtatgcatg cgctgtcatt tgcaaggtaa tgccgtgttg aacagcgata
    46261 aatcattttt cgattttaag cccggtatga aactgagtga tgtgatgaat gtgtttgttc
    46321 cgctttatga gggtaacggt aatgcacata tcatggcatc acatgccgaa aggcttaaac
    46381 aaagcagatg ttatatagaa acagcaaata aaaatcatgt taatcatcag ccttctgagt
    46441 taaggcccgg aaaaaatgct ttaacctgta taacctgcca caatccgcat gtcagcgtgc
    46501 ggcaaactaa tgacgaccat ttcaatgcag tatgtaaaaa ttgtcatcat acagaaaata
    46561 gtttatgcac tgagaagttg gccgtccgca gtaagtcaaa tgataactgc attagctgcc
    46621 acatgacatt ttccggcaca tcagatattc ctcacgtcag catacacgac cataaaatta
    46681 gtattccgtc aaaagacaat acagcgcatg agcataaagt gtttaaagga atttattgtg
    46741 tcaacaatcc agcggctgac aggaagagta ggggtagagc ctttatagct tattttgaaa
    46801 gatatggtta tgatacaagt gtgctcgatt ctgcactaaa ctgtttttct gatgcaggaa
    46861 atgtttctct cagagaaaac attaatgaat tagttcagat ttattttctg aagaatagtt
    46921 ttgcaaaagt tattgagctt acttcggcaa tgcctgatat gttgaagttg ctgaataaaa
    46981 aatcttattc aaatacacat gcctggacag cctatcggat aggagcatct tttcaggcaa
    47041 ttggaaaaat tgaagcagca ttaccttttt ataaacgtgc tgcagaattg gcaccctatc
    47101 atttagatgt tttgaataaa tatgccacag cactttctat tgccggtaat gttaaagaag
    47161 cgcaaaaaat atatgagttt attttaaaag aatatccaaa acatgtttcg gcactaacta
    47221 atcttggatt tatttttctc tccgaaaaaa atgacccgca gcaggcattg gcttattata
    47281 acaaagcatt ggcactcaat cctgattatg aacctgcttt aatgaacatg gcagggctaa
    47341 tgcattatat gaatagaaat aaagaggcta ttgaatttgt gaaacgcatc ttaaaaatta
    47401 gccccaacaa cgaaggggca agacaattgt taaaccaact gaagtcaaca taaatgaaga
    47461 aaatactttt tttagtttta accttacttt tattagcagc cgcagttgtt gcatatattt
    47521 tttatcagag aatattttct ccgaatgtaa agctaaaaga ccataaaact tttttttata
    47581 tcagaacagg aagcgatttc aatcaggtag caacagaact taccgatcag catattttaa
    47641 tcaatactga atctttcaaa tggcttgcag aaagaatgaa ttatacagaa cgtgtaattc
    47701 ccggtcgcta tgagattact gacaatatga acaacaggca gttgttgcag ttgctacgct
    47761 ccggaaaaca ggtccccatt aaaattactt ttaataatat ccgaaccaag tcagaccttg
    47821 caagtcgaat cagcaaacaa attgaagcag acagcacaca aattgcagct atgctcagtg
    47881 atagccttct gttaaaagaa gaagcactga acagcgacaa tgttttatgt ctgtttattc
    47941 ccaacactta tgaaatgtat tggaatactt cagcaacaga tttttttaaa agaatgaaaa
    48001 gggagtatga taatttttgg aatacatctc gcaggcagaa agcagaacag caagggttaa
    48061 cacctgttca ggtaagtata ttggcgtcta ttgtgcaatc agaaacaaag caaaatgccg
    48121 agaagccacg tgtagcggga gtctatctta acagactcag acaaaactgg aaactggagg
    48181 cagatccaac acttgttttt gcattaggtg acttttcgat taaacgagtg ctgaatgaat
    48241 ataaaactat tgattcgcca tacaatactt ataaatattt cggactacct cccggcccta
    48301 tttgtttacc tgatatttca agtattgatg ctgttctcaa tgctgaaagc caccaattta
    48361 tgtatttctg tgctcgcgaa gatatgagtg gttttcattc ttttgcgaaa gattataata
    48421 cgcatctgct caatgccaga cgctatcagg cagaactgaa caggcgtaac attcggaatt
    48481 aattcactgt aaaacggtat ttttacgcca atataatgag aaaagggttt ttaatagtta
    48541 ttgcagcact ttttattact gctgatgttt ctgccaatca ggtggctgca ctgctccgtg
    48601 ttaaacgtag tgagcagccc gatacgctcg gatttaattt ggtcgaacag cttactaaaa
    48661 ttacctatca gctcattcaa cagaataaaa ttaaattgtg ggattcacca tccaaagaaa
    48721 ttcaaatcac cgcagcatca cttcaggaaa ttgaacaatc atcatcaacg tcatttaagg
    48781 aggaggatgt tatttatatt tatgaaagtt ggactgaagc taaacgagaa atacaaacca
    48841 aaacaatagg aataggcttt tcaaatcgcg atagccgtaa tcaggaagtg tcctatggtt
    48901 atgttgatta tgcagattta gttcgttttt tgaatgagaa gcctgcagaa gtaaatgcca
    48961 atggcaacta tggtgagaca cctgcatttt atctttcagc taaaaaattt aatttcagtt
    49021 tagtgcagtt taataataaa gtaatccaga gtgcagatga atctcaaaag acattgaaga
    49081 gttttgtggg aagatcagga ttctctgttc ctcagtcgga ttttagtatt gaagagtcca
    49141 agaacattgt ttatttggtg gacagaaaac ccggtggtga tacattactg tcaaataaca
    49201 gcagcagttt ttttagtgct gtagagaact atttactatc caacaaagag gagtttttta
    49261 atctcggtgg cgacaaggtt cagaattttg ttagtggaaa acaaagtctt agagttaccg
    49321 ctattgaagt gaatgaggtg tggaagaaaa acaaaggaca gatttattac gagccaaaaa
    49381 gtattcgttt ttatgtaaac gattctgcat taaacattat tccgctttct gaaatgacta
    49441 tgatggattt ggaaatcaat ggtcagaaat tggtgctcac cttgctcgat aaaaaattta
    49501 atttcacaat aacaagaatt aactcacagg atattgcctt gcgacaggct tttgtttatc
    49561 tcaaagcact tcgcgagtat cactggaatc agctgacaga attcgtaaaa tattattgaa
    49621 cattataaaa atttatggca acaaaaatta aattcggtac cgatggatgg cgtgcaatca
    49681 ttgcacagga ctttacagta gaaaatgtta cacgtgtggc ggaggctaca gcacagtggc
    49741 taaaggcaaa gggtagtaat ccttcagtag ttgtaggcaa cgactgtcgt tttgcaggaa
    49801 atctttttgc tgaaaccact gccaaggtgt tatgtgcaaa cggaattcat gtaaaactgg
    49861 cacccaattt tgtttcaact ccaatggtgt cgttaggtac attacactac aaagcagacc
    49921 tagggattat acttactgca agtcacaacc caccatcata caatggtttt aaactcaaag
    49981 gctcctatgg cggaccttta ttgcctgcac aggtttcgga gatagaagat attatcaaag
    50041 accaatctat catagatacc aacacacttt cactggatga ttttgctgcc aaaggattgt
    50101 tgcaatgggt tgatttggaa acattgtata tagaccatgt aaaagcaaac tttgaccttg
    50161 acgccattaa gaaaagcggt aaaaaatggg catatgatgc catgtttggt gctggacaaa
    50221 atgtaatgaa aaaacttttt ccggaaatta cactgctgca ctgcgatcac aatcccggct
    50281 ttcacggtca ggcccccgaa cctattcata aaaacctgac agagttttct acccttatta
    50341 aaaatgccgg tgatattgcc tgtggattag ttaccgatgg cgatgccgac cgcattggct
    50401 tgtatgatga caaaggaaat tttgttgact cgcatcatat cattctttta ctcatccact
    50461 acctgcataa atacaaagga atgaacggaa aagtgtgtac agcattttct accacaccca
    50521 aggtgcaaac actttgcaaa cactacggcc tgcccattga cgtagtaaag attg
//
LOCUS       DAOQNP010000011        49959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_200_length_49959_cov_10.444253, whole genome shotgun sequence.
ACCESSION   DAOQNP010000011 DAOQNP010000000
VERSION     DAOQNP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 49959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49959
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_49959_cov_10.444253"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>1446
                     /locus_tag="PKY09_02765"
     CDS             112..>1446
                     /locus_tag="PKY09_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11806.1"
                     /translation="MTTKSFWSQQAILRIRSNKRLFLGCLLAGSVGASAYLFLMPKVY
                     ESQAILKNIDPDTQSTAIAAEMIRSPKTIQQSVKGMGLNVEYFVSKNFQTSECFDNKP
                     VSVSYNLKDDSFSQQNFSLQRDGEKTYILEYDDQTGHQQKRGSYNELLDLGSLAVTIE
                     KTPYLNLLDAKDEVSFTIYSDSRLANKIAEQNLSCTIEDGGVIKLNSQSTNPAKAYRI
                     ASAVSKGYGNAGQQANESHSLANVQLINNQLDRVSQELDNAQRELSHFQSENNAYEMP
                     QQAGAVLNTVSQLQIQKVEADMQIAALDNLSDYMRKNRNVNTISPEYGTITDIIYTET
                     YLKLNDKVAERQQLLEQGADLSKVDKEISSLKEMLAESIRNTRKKLTIRQETLAAAIV
                     SAKDRIQNLPQTEEKLQQINRSIYLYTKLYDFLIQKRAEAMVEAPVLPPSSYI"
     assembly_gap    1447..1546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1547..2007
                     /locus_tag="PKY09_02770"
     CDS             <1547..2007
                     /locus_tag="PKY09_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11807.1"
                     /translation="KNLHQAVQITNYENVDLLCAGYLAHGVNTLLINDKTGNILADMK
                     TLYDYVIFDTPETSNYMDAVPLMKMSDLNMFVVKANSATFDQLEYASQMKKDFQVENL
                     FMIINGMTDTMNHSGMQTSGKVKQIRRREQAEAEIRFMPRMMKKAALWFY"
     gene            2018..2752
                     /locus_tag="PKY09_02775"
     CDS             2018..2752
                     /locus_tag="PKY09_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11808.1"
                     /translation="MGDKAKFLNHFFERISEIRYATMGLTVLHPEEIAEDIIQIAINR
                     EDTRKILTVLEFSPYLKRVRLEEKPFYNFIHLKLSNGQWIQIQLIYGFVRKGIQFMNE
                     KEVLNDSTVNSGGIKVTSQHLNFEYVWLIHCLNKTNVPNHQQLFFSQYNRNERSAIFA
                     HIRSRYFLELNVLDELYTFHKKTNRQVYEKIIRRKENTWAKRNIRKTLYALYSALNLI
                     KTKSIKVQFKGSGYHPFSKTERARVF"
     gene            complement(2749..3156)
                     /locus_tag="PKY09_02780"
     CDS             complement(2749..3156)
                     /locus_tag="PKY09_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNB11809.1"
                     /translation="MNRPVRVLISKVGLDGHDRGAKVIASFLRDAGMEVIYTGLRQTP
                     QMVVNTALQEDVDVIGVSILSGAHLTVFPKIMELLKEKGMTDVLVTGGGIIPDADMKK
                     LNDLGVGKLFAPGTNTNEIVQYINQWVEQNRKN"
     gene            3282..3764
                     /locus_tag="PKY09_02785"
     CDS             3282..3764
                     /locus_tag="PKY09_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNB11810.1"
                     /translation="MKKLVVQLFLLFLFGTGVAQDTNNNIPKPQSIVQQPGTFILHRK
                     IAIVEGPAWVTGDAAARVYCTQLKDSIGLHLAIVNAMREGTFENAINLKKIKDASLGN
                     DGYRIKIKKPAIIVEANNDTGLTNALQYLFQIIPRKFFEDKNPKAKIPLSCSEITCKF
                     "
     gene            complement(3930..4118)
                     /locus_tag="PKY09_02790"
     CDS             complement(3930..4118)
                     /locus_tag="PKY09_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11811.1"
                     /translation="MRKISFVSGSVAGSLTVLGGLFKIMHWPGANIMLVLGLGTLAFI
                     FIPIAAKYWYNKNLVKPI"
     gene            4410..5204
                     /locus_tag="PKY09_02795"
     CDS             4410..5204
                     /locus_tag="PKY09_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNB11812.1"
                     /translation="MEVFGYACAILMGLSLGLIGGGGSILTVPILVYLFSIDAVLATG
                     YSLFIVGFTSLIGSFSHIRMGNVHLRTAVVFGIPSIIAVFITRAFIVPAIPQDLFNIG
                     TLQITKPIALLLLFAVIMLLASYSMIRRKKQKPDYDKVQYNYPLIIIEGFVVGTITGL
                     VGAGGGFLIVPALVLLAHLPMKKAIGSSLLIIATNSLLGFSVSLFHSDSIDWHLLLTF
                     SAIAILGIIIGSLMSKKISNEKLKPAFGWFVLIMGIYIILKETLLK"
     gene            5303..5866
                     /locus_tag="PKY09_02800"
     CDS             5303..5866
                     /locus_tag="PKY09_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB11813.1"
                     /translation="MLELILKPWPWYVAGPLIGLIVPALLILGNKSFGISSSFRHVCA
                     ACVPSNIPFFKYDWKKERWNLFFVAGIMAGGFIAVNLLTASTTVTINSSLATSLHEKG
                     ITDFSGLVPADLFNWNSLFTLRGFIMMVVGGFLVGFGTRYAGGCTSGHAIMGLSTLQL
                     PSLIATCCFMAGGFIMANLILPYILSL"
     gene            5877..6389
                     /locus_tag="PKY09_02805"
     CDS             5877..6389
                     /locus_tag="PKY09_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNB11814.1"
                     /translation="MKTVVTLEKDRNTDFETRSLDTICVNESHLEHSWVHNLKYSLMG
                     ILFGIILVKAEVISWFRIQEMFRLQSFHMYGIIGSAVAVGALSVWLIRKFNIKTFYGE
                     EITLHPKEFNKGQIWGGLFFGLGWAITGACPGPLFATIGSGATVIVVSLLSAIAGTWV
                     YGKFRDKLPH"
     gene            6521..7192
                     /locus_tag="PKY09_02810"
     CDS             6521..7192
                     /locus_tag="PKY09_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNB11815.1"
                     /translation="MISVSEIIIRLSLASFFGALIGLERERKHWAAGLRTHMLVCVGS
                     CLMMIVSAFGFSDIIGTPDITLDPSRIAAQVVTGIGFIGAGAILFSNNKTIRGLTTAA
                     GIWTVAGIGLATGGGMYLAAAVATAIAIIILWGLQPLEKMYFEKFKQKSLRITASHDF
                     GNTEFLTALLRENGIALISLNFTRTNEDLIFQIQLEHADNGTIESVINILKKNSAIKE
                     IQWLN"
     gene            7354..7821
                     /locus_tag="PKY09_02815"
     CDS             7354..7821
                     /locus_tag="PKY09_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNB11816.1"
                     /translation="MINIGINKKQAKQLAAKLNLLLADYMVFYQTTRGLHWNIRGEKF
                     FELHLKFEELYNNLLVKVDEIAERILTLDETPLHTFSDYNKASQLKEIKNVSDPSKSI
                     KAILDAFETILIKQRDILKMSADADDEGTNALMSDYISEQEKLVWMYSAYYNK"
     gene            7823..8140
                     /gene="trxA"
                     /locus_tag="PKY09_02820"
     CDS             7823..8140
                     /gene="trxA"
                     /locus_tag="PKY09_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB11817.1"
                     /translation="METTTQNRTFNDIINGNKPVLVDFFADWCGPCKMMAPILHEVKN
                     EVGEKAVILKIDVDKNPAVAAAYEIQGVPTLILFKSGKVLWRQSGVVNKPQLLRIIEQ
                     NLG"
     gene            complement(8192..9079)
                     /locus_tag="PKY09_02825"
     CDS             complement(8192..9079)
                     /locus_tag="PKY09_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNB11818.1"
                     /translation="MLPIRKLFFIGFTFITLHATAQDDLMKMLSESGEKEKKEEVTAT
                     FKSTRLINGHSVENNKQGVLQLMILHRFGQINSGGYEFFGLDNATMRLGFDYGVTDWL
                     NVGIGRSTFEKMYDGLLKVKFLKQRKGGTPVTLTAVASMQLNSLKFTDPQRKNYFSSR
                     LTYSYQLLAASKLSNAFSIQLMPTLIHRNLVPTANEKNDVFALGGGGRIKISKSIAFT
                     AEYYYVFPDQISDTYKNSLAVGFDIETGGHVFQIHFTNSRSMVEKGFIAETTGDWLDG
                     GIHFGFNLNRVFTVYYPKE"
     gene            complement(9090..9641)
                     /locus_tag="PKY09_02830"
     CDS             complement(9090..9641)
                     /locus_tag="PKY09_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB11819.1"
                     /translation="MKKFILCVLITGMHYAQAQNVFTTNAGTISFFSEAPLENIDATS
                     KKAHCILNITTGDIVSLVAIRSFKFEKALMEEHFNEKYLESDKYKDATFKGKIQEQLV
                     IDRDTAYQVTVNGNITIHGIEKPANYKANYSFKGGVPAIEGSFNVALKDHNVEIPKLV
                     IQNIAEVVKVTCKFELAPYQKKN"
     gene            complement(9652..10023)
                     /locus_tag="PKY09_02835"
     CDS             complement(9652..10023)
                     /locus_tag="PKY09_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11820.1"
                     /translation="MNKSLILISGIFIALCISSCYYDVEDELYPNTGNCDTLNITYSQ
                     TVAPIVSSNCKICHSASMAQGNVVIDTYAAIKIYADNGKLLGSLNHDSGYEPMPKNQN
                     KLSDCNIKKISIWINQGAPNN"
     gene            complement(10030..10731)
                     /locus_tag="PKY09_02840"
     CDS             complement(10030..10731)
                     /locus_tag="PKY09_02840"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11821.1"
                     /translation="MNKIIFFWVMLLTIAATSCKHEPQDLAIATDDSTATEDCNSDTI
                     YFKNTIQPIINASCAYSGCHDVTTAEDGVILDTYANIIQTGKVKAGKPEDSKLYKVLV
                     ENDPDDIMPPPPGSPLSSVQINDIYQWILQGAKNNECLGCDSVNVTFAGNILPIMQTI
                     CQTCHSGSAPLGNLSLVTYDDVSAVAMTGQLVGAVTHADGYFTMPDKEDSTKYIPNCK
                     IKQIIKWVNDGYPDN"
     gene            10888..11157
                     /locus_tag="PKY09_02845"
     CDS             10888..11157
                     /locus_tag="PKY09_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNB11822.1"
                     /translation="MNGMILFLDNLGGGELLVIMVFVLFFFGSKKVPDLARGLGRASR
                     EFKDAMNGVQREIEQSMQTPPPPPQKSVEKPVTPEKNGGVDEKTD"
     gene            11275..13464
                     /locus_tag="PKY09_02850"
     CDS             11275..13464
                     /locus_tag="PKY09_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNB11823.1"
                     /translation="MSNVRFRALDATNGRIPVKITPPSVKVSDYFATNVFTKEAMFKF
                     LPKNAFNAVMSAIETGEKIDRKIAGEIAHGMKEWAISKGATHYTHWFQPLTGATAEKH
                     DSFFDISNDNSFEHFSGSSLVQQEPDASSFPSGGIRNTFEARGYTAWDPGSPAFIIES
                     PNGRTLCIPTIYVSYTGETLDYKAPLLKALHTLNEVAVPVAQYFDKDITRVSTTLGIE
                     QEYFLVDTSLFYARPDLAITGRTVFGHSPAKGQQLEDHYFGSIPDRVNAFMNDFEIEA
                     YKLGIPLKTRHNEVAPCQFECAPIFEEINLAVDHNQLLMDLMDKVARRHHFKVLFHEK
                     PYAGINGSGKHNNWSMVTNTGKNLLSPGGNPKSNLMFLTFFINTIKAVHEHADLLRAS
                     IASAANDHRLGANEAPPAIMSVFLGAQLSSILDNIEKKVGSSKMSVEDKTALKLNIGK
                     IPEILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSNSMAILNTIVADQLRKFRKEVD
                     ALIKKNVKKDEAILRVLRQYIVETKSIRFEGNGYSEEWVKEAKKRGLSNHQTTPVALD
                     AMISKKSLKLFEDNHIFTHREAEARHEIMLETYIKKIEIESRVIGDLAQNHIIPAAFR
                     YQNFLAETVDNLKDCDLKAETYSTQLQLIKEISAHVNAIKKNVDAMLASRDKGNKIAD
                     IRDKAIFYCDKVKVFFTTIREHVDALEMIIDDEIWPMVKYRELVTIR"
     gene            complement(13614..14189)
                     /gene="nadD"
                     /locus_tag="PKY09_02855"
     CDS             complement(13614..14189)
                     /gene="nadD"
                     /locus_tag="PKY09_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNB11824.1"
                     /translation="MKVGLFFGSFNPVHNGHMMIANYFAEFTDLEQVWLVVSPHNPLK
                     PAGSLLNDYQRLHLVEAAIGSYKKIKISKIEFGLPKPSYTINTLVHLNEKFPQHEFVL
                     IMGSDNLVTFHKWKNFEQILENYKIYIYPRPDSDGGELKNHPSVKIIQAPLIEISSTF
                     IRQAIKEGKDVRFYLPESVYDYIDEMNFYKK"
     gene            complement(14190..15698)
                     /locus_tag="PKY09_02860"
     CDS             complement(14190..15698)
                     /locus_tag="PKY09_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNB11825.1"
                     /translation="MVCRILIAAFFIFFNSNLFAQTDSTVTNGFKTFYYHNGNKSSEG
                     NFVNGKPEGLWKSYFENGKLKSEGSRHNNKPEGLWKFYNENGKIASELTYNNGLKNGL
                     QRYYFSDGKLKQEEEVKEGIRQGWVKDFGENGKVVNIVPFKNGVEDGIAKSYAEDGRL
                     ITITTYHEGYVRSEERINRIDKFGLKQGVWKVFFDNDKVKEETEYLDGKKNGFYRVYN
                     NDGSLSLSERYKNDELVIDKKSTTKFEVKRDFYLGGKVKSIVNVINDKRQGVEKIYDE
                     KGNVISGKLFEDDKPIAEGITDDNGNRQGEWKFFYDDGSVHSIGKYKDGKRIDEWKFL
                     FPNGAVQQIGNYKNGLPEGEWKWYFKNGEIRRDESFINGKEEGVSKEYNDSLQLIATG
                     KYVEGKKEGLWKYNNGEYIAEGEYADGLKFGTWHQTYKNGKTAFTGKYIDGLEDGEHI
                     YYHDNGKLHEVRQYKQGQRNGLWKIYDDKGVLILTINYQADKITRIDRIRIN"
     gene            complement(15688..16683)
                     /gene="nadA"
                     /locus_tag="PKY09_02865"
     CDS             complement(15688..16683)
                     /gene="nadA"
                     /locus_tag="PKY09_02865"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNB11826.1"
                     /translation="MTEISEMILKHGYLPESIPASMDLFEEIEKLKKEKNAVILAHYY
                     QESDIQDVADFVGDSLGLAQQAEKTNASMIVFAGVHFMAETAKLLNPDKKVVLPDLNA
                     GCSLANSCPPADFKKFKEQHPGHVVISYINCTAEIKALSDVICTSSNVVKIVESFPPD
                     QKIIFAPDKNLGAYINKKIGRDMVLWDGSCVVHEIFSEQKIIQLKERYPEALVIAHPE
                     CEDHVLRHADFIGSTTGLLKYTKDNPAKAFIVATESGILYQMQKLSPEKIFIPAPPEN
                     NCACNDCPYMKLNTLEKVYLCMKNELPEVIIPKHLQQAALNPLKRMLEISKQHGL"
     gene            17170..17601
                     /gene="rpiB"
                     /locus_tag="PKY09_02870"
     CDS             17170..17601
                     /gene="rpiB"
                     /locus_tag="PKY09_02870"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNB11827.1"
                     /translation="MQSIIIGSDHAGFTLKEAIRKRFSSNFEFTDKGTFSSDSVDYPD
                     YAHAVAGEVEAGKFTFGILLCGSANGVAITANRHNGVRAAICWMPEIAELARKHNNAN
                     IICIPARFVSDETAFLIIEKFFSTEFEGGRHQKRIDKINCV"
     gene            17620..18339
                     /locus_tag="PKY09_02875"
     CDS             17620..18339
                     /locus_tag="PKY09_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB11828.1"
                     /translation="MKLRFILLICVIPFFVKAQDEQDSSAVTTKKQKHENPRFAIAAS
                     GGLSWRLADVSPDENDLTKAYIKDLKSGNGFEISAKYFFNSKLGVGIKYNSHLSKVKY
                     DNMYFSYNNQNYFGTVSDDISINTIAATFSGRFYTRQKTGYFTFDGGIGLMTYKDESK
                     IATNKVNVKGNTLGFNVSAGVGVNITRNISLVIGFNFLNGTLSQYDVTENGIKERVTL
                     NESEYQSLSHIDVFEGLVITF"
     gene            complement(18342..19109)
                     /locus_tag="PKY09_02880"
     CDS             complement(18342..19109)
                     /locus_tag="PKY09_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNB11829.1"
                     /translation="MNKIAKYIMLDILKSKVVIAYLVFLMVTGFSIFNLDENTSKGLI
                     SLLNIILFIVPLVCLIYATIYIFNSSEFIELLTAQPVSRKKLITSIYAGVVLSLTLAF
                     LIGIGLPILIFTPDATGLIFLLAGILLTLIFTSLAFLGSIITKDKAKGIGVAIFAWLF
                     FTILYDGLLLLLMFQFGDYPIEKPLMILTTFNPVDLARILILIHTDYAALMGYTGALF
                     NEFLGSQKGTITILIVLLIWIIVPALIAVHKFRKKDL"
     gene            complement(19102..19815)
                     /locus_tag="PKY09_02885"
     CDS             complement(19102..19815)
                     /locus_tag="PKY09_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB11830.1"
                     /translation="MIESININKTFGKLRVLKDISIKFENNSAVALLGPNASGKTTFI
                     KSVLGMVLPESGTIKVNNTNIKNDDSYRSQIGYMPQIGRYPENMTIGQVFEMIIDMRK
                     EQMNHTDTDLLDALNMKSVFHKRMGTLSGGTRQKVSACLAFLFRPKIIILDEPTAGLD
                     PLSSEILKEKVKTEKNNGKLIIITSHILSELDELVSEIVYFREGELMFHKNLEELQLQ
                     TGEKSISKVITTILKKNHE"
     gene            complement(19812..21071)
                     /gene="nosD"
                     /locus_tag="PKY09_02890"
     CDS             complement(19812..21071)
                     /gene="nosD"
                     /locus_tag="PKY09_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNB11831.1"
                     /translation="MKSNKTLSVVASCFLILLFFSGKSQAGILKIGKNFSIKSIKEGV
                     VKAQPFDTLYIHSGLYKEQNILIEKSLSLIGVENPVLDGENNFEVLTINANSVLVSGL
                     TIKNSGYSSMKDLAGIKVMNSRQFLIKNNTLINSYFAIHIANCDNFRIENNTIRGTPG
                     TDEQSTGNAIHLWKCLYASIKNNDISGHRDGIYFEFVGNSYIEQNNSYNNLRYGLHFM
                     FSNKDSYIDNKFTDNGAGVAVMFSNHISMYRNIFSMNRGASAYGLLLKEISDGDIKDN
                     LFENNTTGVLMEGTNRLHITKNSFNKNGYALRVQASCSNNTVHDNNFNGNTFDVATNG
                     SLVLNNFDGNYWDKYEGYDLKRDGIGDVPYRPVSLFSMVTENIPIASIFIRSLIVFLL
                     DRSEKIIPSITPENLVDNKPLMKPVAL"
     gene            complement(21058..21486)
                     /locus_tag="PKY09_02895"
     CDS             complement(21058..21486)
                     /locus_tag="PKY09_02895"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNB11832.1"
                     /translation="MRWTFILISILIVSCTTKPEPLVAGKDACVHCKMPVADTRFGAE
                     IISNKGRIYKFDDINCMLQWSRQSSNVDAGSAKLYVVNYFDQIDLMNAADAVYLKSDN
                     LRTPMNSGFAAFANKEDAEKVLKDDGGSLVDWNFVQNEIK"
     gene            complement(21499..22074)
                     /locus_tag="PKY09_02900"
     CDS             complement(21499..22074)
                     /locus_tag="PKY09_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11833.1"
                     /translation="MTKINGVSRVIIAISSLLIAISFFVPIWRIDLFAPQYPEGLVMK
                     IWLDKLTGDVEVINGLNHYIGMKKISVEMFPEFEFLKYIVAAFIVYGLVIAITGSRKI
                     LFSYLVVSVICGILALYDFYQWGYDYGHNLDPNAPIKVPGMVYQPPILGHKQLLNFDA
                     HSMPDVGGYIVIGSALICAIIWAMGLRKSKS"
     gene            complement(22112..22672)
                     /locus_tag="PKY09_02905"
     CDS             complement(22112..22672)
                     /locus_tag="PKY09_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNB11834.1"
                     /translation="MNTKLLMGVIFAASIAISCNPPAAENQQATTTEETQAGGQEAVI
                     DDDSQKDIVKIAVGSADHSTLVAALKQADYVTSLSNAGPFTVFAPTNEAFNKLPAGTV
                     DDLMKPENKAKLQDILEYHVSLGVFKPEMLTDGQTINQANLKNIAISNKDNKIMINNQ
                     ANIIATIPAANGVIHVIDAVLLPPDK"
     gene            complement(22753..24738)
                     /gene="nosZ"
                     /locus_tag="PKY09_02910"
     CDS             complement(22753..24738)
                     /gene="nosZ"
                     /locus_tag="PKY09_02910"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNB11835.1"
                     /translation="MKQSKIKNVVLASIAVIAIAALPSCKPKNVSTATSGDAAKKAYV
                     APGKYDEFYNIVSGGFSGQMSIYGLPSGRLFRVIPVFSVDPEKGYGYSEESKAMLNTS
                     HGFVPWDDLHHISLSETDGVHDGRWAFGNANNTPRIARIDLKTFRTAEIIEIPNSAGN
                     HSSPFITENSEYVVAGTRFSVPMSNGTTDVPINTYKENFKGTVSFINVDKESGKMGIA
                     FQILLPGVDFDLSRAGKGKSHGWFFFSCYNSERANTLLEVNASKFDKDFIMAVNWKKA
                     EEYVKAGKGVKKNVSYAHNTYDEKTHTANSTIEKEVIMLDPKDCPDMVYLMPCPKSPH
                     GCDTDPTGEYIVGSGKLAAVIPVFSFTKMQEAINAKNFEGDYDGLPVLKYDAVLHGEV
                     KKPGLGPLHTEFDANGNAYTSFFVSSEIVKWRLSDLSVLDRVPTYYSVGHLCVPGGPT
                     KKPWGKYVIAYNKITKDRYLPTGPELTQSAQLYDISGDKMQLILDFPTIGEPHYAEAI
                     PAELLSKNSTKIYKIEENQHPYVTKGEKEAKVERKGNEVHVYMSSIRSHFVPDNIEGV
                     KLGDVVYFHVTNLEQDWDVPHGFAVKGANNAELLIMPGETSTLMWKPDRVGVFPFYCT
                     DFCSALHQEMQGYIRISPANSNVPLTFSTGTNQPPIN"
     gene            complement(24761..25273)
                     /locus_tag="PKY09_02915"
     CDS             complement(24761..25273)
                     /locus_tag="PKY09_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB11836.1"
                     /translation="MKSIQKHLINLMIAGMGITLFAACGGSATKEEKAAESKDMNQYY
                     EEEKQKEDAKVADDGKGFGKFTDVKVDPKLDKQMADAGKSIYDVKCSACHKLTDEKLV
                     GPGWKGVTGKRTAEWIMNFSTNPDEMLDKDAIAKGLLEECLVRMPNQGLSDDDARHIY
                     EYMRENDGVK"
     gene            complement(25491..25868)
                     /locus_tag="PKY09_02920"
     CDS             complement(25491..25868)
                     /locus_tag="PKY09_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11837.1"
                     /translation="MYTNQYKAGCCNIGEAEIRIRQKFLIFFLLISILLTITVHFYHS
                     LWVFYLLFLTTLATIILFVEVVSRFCVLFAIFSLHNFHEPGNLDAIDDRHCKKKDRLR
                     ALYIIMGAIVVALLYTCLISVFC"
     gene            25991..27370
                     /locus_tag="PKY09_02925"
     CDS             25991..27370
                     /locus_tag="PKY09_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB11838.1"
                     /translation="MKKTAVIFFILFSQFITVFGQSQDSLILRKIYNESLEHGQAYHY
                     LKTLCKQIGHRLSGSPQADKAVLWAQKAMTDFGADTVFLQDVMVPHWVRGEKENASII
                     SSKNNQSIPLNVLALGGSVGTDGKTLTANVVEVSSLDELDKLGAAKCSGKIVFINQRM
                     DATKIMTFDAYGPAVAIRWAGAKRAAPLGAVAVIIRSVNPSLDDFPHTGTLHYPDTIK
                     KIPACAISTNDAEKLSAMIKSDPQLKIKLKMNCKQLPDAPSHNVISEIKGTDANAGYL
                     IVGGHLDSWDTGEGAHDDGAGVVQAMEVLNLFKKAGIKPRHTIRAVAFMNEENGGKGG
                     EYYAKNALQKGEKHIAAIESDCGGFTPRGFFLQGDSLKKEKIRSWKNLFEPYQIHSFN
                     FDGGGADIGHLKNQNTFLLGLLPDTQRYFDIHHSPNDVYENVNRRELQLGAATMAAMI
                     YLIDKYGLE"
     gene            27370..28509
                     /locus_tag="PKY09_02930"
     CDS             27370..28509
                     /locus_tag="PKY09_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB11839.1"
                     /translation="MLLKETELKQNAAAYADQQKNISSVAPQIFLNRKKEIFSDYELA
                     TRRAAYYRWKSTESLDKLLIAFEANAIKAGIKVLWAVDVAQAHLELNAVVNKFQQSKI
                     HIHTCYNELETEKLNQPSSINTKNKISICDAIFVAAEPATVACFSDKNFYESDVKILL
                     ASVDKLISSVNELELLMSLQSLQKSNSGESFSLITSAGHNSQRQDVYVLIFDNGRSKL
                     LESQTERQMLHCIDCGICQVVCPVTESIGITGGNENNYGPPSYIRAVFDGGQREYGHY
                     AGLCNMCGKCNVHCPVHIDFKNSFLHLRHENVQQKHRSQKERLFYMVWRKTMLKREFM
                     NWKTVNPLRYAVETLFLKSKTGIRKLPEPATKSFNQMWRDKMGMK"
     gene            complement(28511..29548)
                     /locus_tag="PKY09_02935"
     CDS             complement(28511..29548)
                     /locus_tag="PKY09_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNB11840.1"
                     /translation="MQLRNLVSKQLLKQRLAEEKFNRKTVSFYRYFKIEDVEDFRNHL
                     YIDFEALNIFGRVYVATEGINAQISVPEHNWDVFKNYLEKQPLLKSMPLKIAIEDNGK
                     SFYKLIVRIKSKIVADGLNDNTFDVTNVGNHLSAKEFNEAMQQSGTIVIDMRNHYESE
                     VGFFKDALKPDADTFRDALPQAIEMTKTNKDNKILLYCTGGIRCEKASAYFKHHGYKD
                     VNQLYGGIIQYAREVNAEHLENKFLGKNFVFDERMGERISDDIVSTCHQCGAKCDTHV
                     NCANDDCHLLFIQCDSCKTTYEGCCTPECKAIINLPEEEQIRLRKGRAKKHAHAVYKS
                     RLRPDLKKILK"
     gene            complement(29584..29823)
                     /locus_tag="PKY09_02940"
     CDS             complement(29584..29823)
                     /locus_tag="PKY09_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11841.1"
                     /translation="MKRILILLIIFLSALTFQNVNAQCSVCRAGAESSVEQGYKAGKG
                     LNKGILYLMAVPYVMVGVGFYFFYNKIKKKETKEV"
     gene            complement(29825..32872)
                     /gene="infB"
                     /locus_tag="PKY09_02945"
     CDS             complement(29825..32872)
                     /gene="infB"
                     /locus_tag="PKY09_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNB11842.1"
                     /translation="MSDEKKDVRLSKATKDLGVGIDHIIEFLNKNGIQVEKNPNTKIS
                     ADAFSLLTKEFAPDMHARQEANEITREKQKRENTIIEVKDDSGKSVSESETDDELADK
                     LLSIKQQATEKQATAKAKETKEKTTKAKAVKAEEPAPVAEVEKPVAAKEKTKEPEVVK
                     AKASKLEGPKIQGKIDLESTDKKKKKTTKKEEIAAEEPKKTTAKKKAEVEVKEEEPKK
                     KKPTKKKEDEEVKQIEEVAAVTTKEEPVAVVEAQPEVVTEATPVTAETDKTVTVETEQ
                     KTDDTTPPELFRTDREKLTGPTILGKINLPVDPPKKKPVATSSEDDRKKKRKRKDKKV
                     DIGKAGNQPHQGGAPRTAGGQRPGGGHGDFKRRNDFRNKPAEAKPEVTDKEIQDQIKA
                     TLARLSGGGKSKSSKLKKLKREQVQEELQSQLEQESNEKKVIKVTEFVTASELSKLLG
                     VPINQIISTCMSLGMVVSINQRLDAETLSVVAEEFGATVEFVSVEVQEAIKDVEDKPE
                     DLKPRAPIVTVMGHVDHGKTSLLDYIRKTNVISGEAGGITQHIGAYEVQLDNGKKIAF
                     LDTPGHEAFTAMRARGAKITDVAIIVIAADDSVMPQTIEAINHAQAAGVPIVFAINKI
                     DKPGANPEKIKEQLSKMNILVEDWGGKFQVQEISAKQGLNVDLLLEKVLLEAELLDLK
                     ANPNKNAIGTVVESSLDKGRGYIATVLVQSGSLKTGDVVLAGCYSGRVKAMFNERGNK
                     RIHAGPATPVLILGLNGAPQAGDQFNVMDDEREAREIANKRLQLQREQGIRTHKHITL
                     DEIGRRLAIGNFKELNVIVKGDVDGSIEALADSLLKLSTAEVQVNVIHKSVGAITESD
                     VLLASASNAIIVGFQVRPSVNARRLAEKEEIEIRLYSIIYDAINEIKTAMEGMLAPEF
                     EEKIVCNIEVRETFKIPKVGTIAGCMVLDGKMTRNTAIRVIRDGIVAYTGKLASLKRF
                     KDDVKEVSAGFECGLNIEGYNDIKVGDIIEGYEQVAVKRTLTV"
     gene            complement(32936..34174)
                     /gene="nusA"
                     /locus_tag="PKY09_02950"
     CDS             complement(32936..34174)
                     /gene="nusA"
                     /locus_tag="PKY09_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNB11843.1"
                     /translation="MDHAVLIESFSEFKDSKNIDRATLMSIMEDVFRAMVRKKYGEDN
                     NVDVVINTDKGDVEMYRNRTIVEDGMVEDDNTQIALSDALKIESDFEVGEDVTEQILL
                     DDFGRRAVLAARQNLVARIMELEKESIYQKYKDRVGEIVTGEVYQVWKKEIMVMDDEG
                     NELSLPKSEQIPADFFKKGDTVRAVVLRVEMQGGSPKIILSRTAPVFLERLFELEVPE
                     VFDGLITIKNIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INYTNNLSLYIQRALSPAKISEIKLDDDTKKAAVYMKPDQVSLAIGKGGNNIKLAGKL
                     TGFEIDVYRDSDVEDDEDVDLDEFTDEIEPWIIEEFKKIGCDSAKDVLAISIDDLVKR
                     TDLEEETIREVVRILNEEFE"
     gene            complement(34187..34642)
                     /locus_tag="PKY09_02955"
     CDS             complement(34187..34642)
                     /locus_tag="PKY09_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNB11844.1"
                     /translation="MIAEQIHELIKVYLADKDLFLVDIKVTPGKITVLVDKPAGITID
                     ECVSLNRYLYKEMESSGVFENKEFEVGSPGMDQPFKVLGQYKRRIGRPVKILLKDGKQ
                     HAGILVDVNEQELQLLETIKEKIAGKKVEKQITTTMPMQNIKETKLEFK"
     gene            34778..35500
                     /locus_tag="PKY09_02960"
     CDS             34778..35500
                     /locus_tag="PKY09_02960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB11845.1"
                     /translation="MADNLNLEKILFLDIETVPQFESYARLPGFDKDLWDYKALHLKK
                     VDTDTAESLYPRAGIYAEFGKIVCISVGWFMTESNRRYFIVKSFWGDDETDLLLKFRE
                     LLTNNYNKSSHLLCAHNGKEFDFPYLCRRMLLNKVRIPDILNIGGKKPWEVQHLDTMD
                     MWRFGDYKSYTPLKLLTHIFNIPSPKDEIDGSEVWHVYWKDKDLRRIVKYCQKDVVAV
                     ARLLLALRNEGELNDSEIVITD"
     gene            complement(35497..36396)
                     /locus_tag="PKY09_02965"
     CDS             complement(35497..36396)
                     /locus_tag="PKY09_02965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB11846.1"
                     /translation="MEILNECPACGTNAKIEYLTCKDFGYSGSYFTINQCVSCKLLFT
                     SPRPDASEIGAYYKSDDYVSHTNKSKGIINRLYPIVRNIAVNQKVRLIKKLIPKNSSV
                     ADLGAGNGYFVKAVLKAGFPIKGYEPSLEAIKLAKETFNTDLHPIETFYTTGNEEFNC
                     ITLWHVLEHIHTLQETLNHIKITLKKNGILIIAVPNPASKDAEYYKEFWAAYDVPRHL
                     YHFTPEALEKLIGSFNFTLLDKKPMLFDSTYVSLLSERYKAKSSKAVQIYNALYRGIH
                     SNMHAAIYTGNYSSLIYIFRKNS"
     gene            complement(36464..38338)
                     /gene="mnmG"
                     /locus_tag="PKY09_02970"
     CDS             complement(36464..38338)
                     /gene="mnmG"
                     /locus_tag="PKY09_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNB11847.1"
                     /translation="MIQGYDIVVVGAGHAGCEAAAAAANMGSRVLLITMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVSDKSAIQFRILNRSKGPAMWSPRTQNDRMLF
                     ASEWRQMLENTPNVDFWQDMVSALIIEDNKLKGVVTSMGHQVRCKAVVLTNGTFLNGI
                     IHIGEKRFGGGRSGEKAATGITEQLVNLGFVSGRMKTGTPPRLDGRSIDYSRLEEQKG
                     DENPGKFSFTNTKPLSAQRSCHITYTNEEVHGILKTGFEKSPMFTGRIQGLGPRYCPS
                     IEDKINRFADKDRHQLFIEPEGWNTVEIYLNGFSSSLPEEVQYAALKKIPGLENVKMF
                     RPGYAIEYDYFPPLQLNLTLETKKIEHLYFAGQINGTTGYEEAACQGLIAGINAHLKL
                     NNQNPLILKRSDAYIGVLIDDLVTKGTDEPYRMFTSRAEFRTLLRQDNADIRLTPVSH
                     QIGLADAQRFSRVTEKKQAVDRIIEACRSESISPAEINSYLNSKESAELSQKVKVIQV
                     LNRPGINLADLIKHSASLSEKLSFINPELKNEVIEQAEIAMKYESYIAKEFEHTVKLN
                     KLENLILHEDFNYDKIKSLSAEARQKLSVVKPQTLGQASRISGVSPSDISILMVYLGR
                     "
     gene            complement(38424..38849)
                     /gene="ybeY"
                     /locus_tag="PKY09_02975"
     CDS             complement(38424..38849)
                     /gene="ybeY"
                     /locus_tag="PKY09_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNB11848.1"
                     /translation="MKPEVNFFKEDVQFRLIGKEAVSSWLADCIKKHHKKPGTLNFIF
                     CSDEFLYSMNIKYLNHKTLTDIITFDYSEKSIISGDIFISIDRVRENAKLLKNSIKDE
                     LHRVMIHGVLHLCGYSDKKAADKEKMTKAENKYLSLRTF"
     gene            complement(38849..39376)
                     /locus_tag="PKY09_02980"
     CDS             complement(38849..39376)
                     /locus_tag="PKY09_02980"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNB11849.1"
                     /translation="MAVSKPFNLQQWVEENRHLLKPPVGNKQVYIDNKDFIVMIVGGP
                     NSRKDYHFEDAEELFYQIEGDIVVKTIQDGKPVDVPIKQGEMFLLPPRVPHSPQRGAN
                     TIGLVVERYRKPDEMDACMWFCEKCNHKLYEEFFPLKDIVNQLPVVMNDFYNSLEKRT
                     CSQCKAVMEPPVPAK"
     gene            complement(39389..39676)
                     /locus_tag="PKY09_02985"
     CDS             complement(39389..39676)
                     /locus_tag="PKY09_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11850.1"
                     /translation="MANPIVDQVSKAANINQEQATKAIEAVSAALKEKLPYLLHNQID
                     ILLTGGNLSDGVKQKFETLKDDLESSAKDLGTKAQEFTEEVSKKLNNLFQK"
     gene            39751..40539
                     /locus_tag="PKY09_02990"
     CDS             39751..40539
                     /locus_tag="PKY09_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB11851.1"
                     /translation="MNLDLTNKKTLVCGSTQGIGKAAAKELCLLGAKVVLLARNEQKL
                     KTVAAELSTLSKSVPDYIVADFSAPENVRQQVREYINKNGGFNILINNAGGPPAGNIT
                     DATAESFIQAFKEHLICNHYLAQECIAGMKEMGYGRIINVISTSVKQPIKGLGVSNTI
                     RAAVGNWSKTMSIELAPFGITVNNVLPGATLTSRLEQIIADKVQKTGKTEKEVVESLT
                     TEIPAKRFANPEETAAAIAFLASPAAGYINGTNVVVDGGRTACL"
     gene            complement(40542..43901)
                     /locus_tag="PKY09_02995"
     CDS             complement(40542..43901)
                     /locus_tag="PKY09_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11852.1"
                     /translation="MQSNKWSFKIDKKLYVIFILLIVITLANAFISTYIIDKSKNITI
                     EIAEVTTPSQSAISDLTQLVNNSWLQATNVAFRKNKAASISELQFINETQYPALKKEL
                     KHLSESWQQKEQLALVGLFEKYDRLIYFESQLIEKARVSTENNVEILETQITPQVQEI
                     TALLSDFNIKKRAETTAKQKEMIQSFIVLMAVVLGLAMVIINFIIVITLFLNKQVVNP
                     LMKIRRYLLQLGKGELPELNIKTPKNAVGEMIDALKMHVSGLNRTAEFANNIGQGKFD
                     YHFEPLSENDVQGKALLDMRSKLKAASEEDASQKWISEGLERISVLNKDYTLDMQVLC
                     NNLIKEVVEYSSACHGTVFLLDTKDESLHEVDLKGIYCRSHQLTDLHIEWIKHQLVKK
                     CIDNNERIETSLLFDIRKDGENKSMPCSVFVMPLFASGRVIGALEIASLNKLTASGLN
                     YLQRLIEPVSASIYAVYSNALTRELLEESIMQSEELVAQKQELAQVNQNLTIKSEELE
                     ISQKELKKQQEELKAANINLEIKAHLLEERSLAVEEARQSLAFKATQLEQTNKFKSAF
                     LANMSHELRTPLNSILILAKLLAGNKNKNLTDKQIEHASVIHKSGSDLLLLINDILDI
                     SKIESGKLEFVFEKMNIETVANDINLLFKEFASEKQINFQVKVWDNCYPEMVSDKLRL
                     EQVIKNLISNALKFTDKNGHVSLDFRKATTEDCKTHNLLFEEDIIAISVTDSGIGIPV
                     EKQKQVFEAFKQADNSTSRKYGGTGLGLTISREIVQILGGEIVLESTPGKGSTFTVFI
                     PFFASCESKHDNPVKKVITQAETEKQETENTKKELPLINTIVSKFPDNDFTDDRNFIT
                     PFDKVILILEKDITFLKTLIDYCHIFNYKAIASQNGETGILLAKSYHPHFILLDHNLP
                     DIDGGTLLKNIRSEQKLCNIPIHIISELKPTQGLINEFNITAFHHKPLNKKQLENVMN
                     QLQAPENIPQEETVVEQAFHESSLSGKTILMADDDLRNIYALTTLIESAGANLIYAYD
                     GKEAINKLEKNPHVDIVLMDDLMPVMNGVDAITEIRKAAQFRNIPVLAVLSGSDAQEK
                     EKCLLAGATDTLSRPLNNDQILNKIKFLLYQ"
     gene            complement(44005..44075)
                     /locus_tag="PKY09_03000"
     tRNA            complement(44005..44075)
                     /locus_tag="PKY09_03000"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44041..44043),aa:Cys,seq:gca)
     gene            complement(44178..44528)
                     /gene="folB"
                     /locus_tag="PKY09_03005"
     CDS             complement(44178..44528)
                     /gene="folB"
                     /locus_tag="PKY09_03005"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNB11853.1"
                     /translation="MHQLILSGIECYAHHGCLSQETVIGGRYRVDITFYADLSIAANS
                     DNLTQTIDYVEVNEMVKTQMAIPSKLIEHVGNRIAQKMKERFTICSKIKVAITKYNPP
                     VKGFISEATFVIEA"
     gene            44613..46148
                     /locus_tag="PKY09_03010"
     CDS             44613..46148
                     /locus_tag="PKY09_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNB11854.1"
                     /translation="MKIRLVLLLTFINLSLLMAQQPITLNVSQPDQDWPNSVTYHIFV
                     RSFSDGNGDGIGDLKGITAKWDYLRKLGAEAILISPVFASPSYHKYDVTDYNKLSPEY
                     GNIESFQYFLKDAHAEGFKVILDIPLNHCSSKHPWFLEAVKNPASAMRNYFLWSNNPP
                     ATEKDKWHLPKDEKGNTVGKAYYYGFFSPEMPDWNYDNTQVVEEAQRILKFWIDLGVD
                     GFRLDAAQHIFNDHIKNVGFWNMISAYVRSINKHIFLVGEVDNSSDVVAPYLKPLDAC
                     FDFDLSREVVQSLKNENAGNFPVRMEKIITHYKTANKDYRDVIFLTNHDQNRLMSEVG
                     NNVDKAKLAANILFTLPGIPYIYYGEEIGMKGEKPDEFIREPMLFAPEKEDEMRPKWM
                     KPKYSTDKTVEPAMVQIKEDQSLWNHYSRIISLRKDNRALQYGQFENSSLSNKSIMAY
                     YRIYNQMRVLIINNVSATAVTLTKEEKDNELVNPIFQTSKQTTVKGNKITVAPYSTII
                     LTN"
     gene            complement(46246..47784)
                     /gene="gltX"
                     /locus_tag="PKY09_03015"
     CDS             complement(46246..47784)
                     /gene="gltX"
                     /locus_tag="PKY09_03015"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNB11855.1"
                     /translation="MQRRIRTRFAPSPTGPLHMGGVRTALYAWLFAKKNAGDFLLRIE
                     DTDQNRFVPGAEQYIIDALHWCGITIDEGVSVGGTHTPYRQSERKEIYRSYAMQLVES
                     GNAYYAFDTAHQLEQQRKNAEEKKETFSYGSATRMKLNNSLALSVDEVKELLSKNVAY
                     AIRLKVPENETITFTDLIRGVVSFDSNLVDDKVLLKSDGMPTYHLAHIVDDYLMEITH
                     AIRGEEWLPSAPAHILIYRYLGLTAQMPHYAHLPLLLKPDGKGKLSKRDGDRLGFPVF
                     PLEWKDPSTGEVSSGYRESGYYPEAFVNMLAMLGWNPGTAQEIFNMNELIAAFSLDHV
                     HKAGARFDLEKTKWYNTQWMLRQPNEAIAKRIKPMVATRTGISADDVRLSDNYLTACV
                     ALLKPRVQFEKEMPEKGIYLFKAPSEFDQTVISKKWKTEFISFFKMLSDEFMTLQDWS
                     SQLAEQTFKTTAEKNNIKPGEVLQLFRVFVSGQAQGVDLFPMIQLLGKDEVIKRLETA
                     LKFV"
     gene            complement(47831..>49959)
                     /locus_tag="PKY09_03020"
     CDS             complement(47831..>49959)
                     /locus_tag="PKY09_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB11856.1"
                     /translation="DYISKPDELKNIGGSAAIISDKGLYFINADGKNPNKPRQVWTQG
                     ETQSNSVWFPTIDSPNQNMTQEISITVDSSFITLSNGLMTSSVKNTDGTRTDTWKQSL
                     PAAPYLTMMAVSNFKVVKDRWRNIEVNYYVDPEYEKYAKDIFGNVPEILDFYSRKLGV
                     DYPWEKLSNVVVHDYVSGAMENTSAILYGEYLHRTKRELLDGNNESTIAHEIFHHWFG
                     DYVTCESWSNIPLNESFATYGEYLWDEYKYGREEADKGGMDDLNQYLAEAKYKQVDLI
                     RFHYNSREDMFDRHSYSKGGRVLHMLRKYVGDDAFFMSLKKYLNDNKFSPVEAHNLRL
                     AFEAVTGQDLNWFFNQWFFAKGHPIIDISYQWNDTLKKQTVIIEQKQNLQETPLYKLP
                     LSIDIYENGKVRREQVTLTQQKEYLFFDCNNKPQLINVDAEKQLLCSKKDNHTNDEWM
                     FQYKHAPLFLDRYEAIDALAKGYEGNSAEAKIIISALDDKSPSIRALALRNIGAAAKA
                     DSAVKNKLQQMVTQDAKAHVRESALSTLYKYFDDSTLDNIAINDSSYNVMTTALEHYI
                     TKNPKQLSELKKYESDNNNNLLYTLSGLYAQYGSDENYDYMINALNVASGFGKMGIIQ
                     NLGTFLEHSSPAVTEKGVDALTDAAKNSSSKFMNMQTSNTLSKLKKFVTNKIKELETK
                     PEYFDQLRDYKKIEEKLTSSITSLKK"
BASE COUNT        15332 a         9096 c         8824 g        16607 t
ORIGIN      
        1 acgactaata cctaaaatgg ttacaccaat ctgtatatta atttacaatc cagaatgtag
       61 tgtaacctaa tatcgttttt ccttgtatag aggttataaa attcagtaaa gatgacaaca
      121 aaatcatttt ggtcacaaca agccattctt cgcatcaggt caaacaaacg attatttctt
      181 ggttgccttt tggcaggcag tgttggagct tcagcctatt tgtttctaat gccaaaggtt
      241 tatgaatcgc aggctatatt aaaaaacatt gaccccgata cacaatcaac tgccattgct
      301 gcagaaatga ttcgttcacc caaaactatt caacaatctg taaaaggtat gggactgaac
      361 gtagaatact ttgtaagcaa aaactttcag acatcagaat gttttgacaa taagcctgtg
      421 agtgtttcat ataacttaaa ggatgattcg ttcagtcagc aaaattttag tctgcaacgc
      481 gatggggaaa aaacttacat acttgaatat gatgatcaga cagggcatca acaaaaacga
      541 ggcagctata atgaattgct cgaccttgga tcgttggctg taaccattga aaaaacacct
      601 tatctgaatt tgcttgatgc taaagatgaa gtttcattta ccatttattc tgatagccgc
      661 ttagccaata aaatagctga gcagaatctg agttgtacta ttgaggatgg tggtgttata
      721 aagcttaaca gccaaagtac taatccggcc aaagcatacc gtatagcaag tgctgtttct
      781 aaaggatatg gaaatgccgg acaacaggct aacgaatcac attccttagc aaatgttcaa
      841 ctcatcaaca atcagcttga tcgtgtttcg caggagttag acaatgcaca acgcgaatta
      901 tcccattttc aaagtgaaaa caatgcctat gaaatgccac aacaggcagg tgctgtgctg
      961 aacactgtta gtcaacttca gattcagaaa gtagaagcag atatgcaaat agctgcatta
     1021 gataatctga gtgattacat gcgcaagaac agaaacgtga acaccatttc acctgagtat
     1081 ggaaccatta cagatattat ttatacagaa acatatctga agctgaatga taaagttgcc
     1141 gagcgtcagc aactgctgga gcaaggtgca gacctttcaa aagttgataa agaaataagc
     1201 tcacttaaag aaatgcttgc tgagagtatc cgtaatacaa gaaaaaaact aactatcaga
     1261 caagaaacgc ttgcagcagc tattgtttcg gctaaagaca gaattcaaaa tctgccacag
     1321 acagaagaaa aactacagca aattaacaga agtatatact tatacaccaa gctttatgat
     1381 tttctgattc agaaaagagc agaggctatg gttgaggcgc ctgttctgcc accatcatca
     1441 tacatannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnataa aaatcttcat
     1561 caggcagtac aaataacgaa ttatgaaaat gttgatctgc tttgtgccgg atatcttgcc
     1621 catggcgtaa acacacttct tataaacgat aaaactggca acattcttgc agatatgaaa
     1681 acgttatacg attacgtgat atttgatacc cccgaaacca gtaattatat ggatgctgtt
     1741 ccgttgatga aaatgagtga tttaaacatg tttgttgtta aagcaaacag tgccactttt
     1801 gatcaacttg aatatgcatc acaaatgaaa aaagactttc aggttgagaa cctatttatg
     1861 attattaatg gcatgacgga taccatgaac cattccggta tgcagacttc gggcaaggta
     1921 aaacaaattc gcaggcgtga acaggcagag gcagaaatca ggtttatgcc aaggatgatg
     1981 aaaaaagcag cgttatggtt ttattgattt aaattttatg ggggataaag caaaattctt
     2041 aaatcacttt tttgagcgta tcagcgaaat cagatatgcc actatgggat taactgtttt
     2101 gcatcccgaa gaaattgcag aagatattat tcagattgca attaaccgtg aagatacacg
     2161 taaaatatta acggtgcttg aattttcacc ctacttaaaa agagtgcgtt tagaagaaaa
     2221 accattttac aatttcattc atctaaagtt gagtaatggc cagtggatac aaattcaact
     2281 tatctatggc tttgtaagaa aaggtattca gtttatgaat gagaaagaag tgttgaatga
     2341 tagtactgtt aacagtggag gaataaaagt taccagccag catctgaatt tcgaatatgt
     2401 gtggctgata cattgtctga acaaaacaaa tgttccgaat catcagcaat tatttttctc
     2461 gcaatataac cgaaatgaac gctctgccat atttgcacac atacgaagca gatatttttt
     2521 agaactcaat gtattggacg aactatatac atttcacaaa aaaaccaatc gtcaggtata
     2581 tgagaaaata atccgcagaa aagaaaatac atgggcaaaa agaaacattc gcaaaactct
     2641 ttatgcctta tacagtgcat taaatcttat aaagacaaaa agtattaaag tgcagtttaa
     2701 aggttctgga tatcatccat tttcaaaaac cgaaagagca agagtctttt agttttttct
     2761 gttttgttca acccattggt taatgtactg aacaatttca ttggtgttag tgccaggagc
     2821 aaacaattta cctacaccca aatcatttag ctttttcata tcggcatcag gaataatacc
     2881 tccgcctgta acaagtacat cggtcattcc tttttctttt agcagttcca taatttttgg
     2941 aaatactgtc aggtgcgcac ctgagagaat tgaaacacct ataacatcaa catcttcttg
     3001 taaggctgtg ttaacaacca tttgtggtgt ttgccggagg cctgtgtaaa taacttccat
     3061 gcctgcatca cgaagaaaag aagctatgac ttttgcacct cgatcgtgac catcgaggcc
     3121 taccttggaa attaatactc taactggtct gttcataagc acaaaaataa caaaggtgca
     3181 ttatctttaa agaacattta tcagtattct tgattttgat atgttcataa tttgatttta
     3241 cagtgcattt ccaaataaac agcaacttta ttttagctgc catgaaaaaa ttagtcgttc
     3301 aattattttt acttttcttg ttcggaaccg gagttgcaca ggatacgaac aataatattc
     3361 caaaacctca aagtattgtt cagcaaccgg gtacttttat tttacaccgt aaaattgcaa
     3421 ttgtagaagg tcctgcctgg gtaacgggtg atgcagcagc aagggtttac tgcacacaac
     3481 taaaagatag tatagggtta caccttgcca ttgtgaatgc tatgcgcgaa ggcacatttg
     3541 aaaatgccat taatctgaaa aaaataaaag atgcctcgtt aggcaatgat ggctaccgga
     3601 taaaaatcaa aaaacccgcc attattgtag aggctaacaa tgacaccgga ctgacgaatg
     3661 cactgcaata tttatttcaa ataataccaa gaaaattttt tgaagacaaa aatccaaaag
     3721 caaaaattcc actcagttgt agtgagatta catgtaagtt ttaaaagtca gaggttaaaa
     3781 agtttcaatg tctgatatat ggatatcaaa cattgaaatt agcgtctatc tctattttgt
     3841 aattataaaa tattgtatta accaatgttt ttaggatagt tgctgatgcg aaaacagaca
     3901 gcttacagat gatttgcttt aaagagattt taaattggtt taaccaaatt tttattgtac
     3961 caatacttag ctgcaattgg aataaaaatg aaagcaagcg ttccaagacc gagcactaac
     4021 ataatattag ctcctggcca atgcataatt ttaaataaac caccaagaac tgtcaacgag
     4081 cctgcaacag atcctgaaac aaatgaaatt tttctcattt taattattta ttaatttagt
     4141 gtttctactc atctggcttt tcgattacgc cccgttttat aagtggttac cggactccac
     4201 atttttatga tttatatcgt attagaatat ttgcactcat atacattttc gggcatgatg
     4261 ctaaaagttt acttttaatt aaaacgtaac aagatatagt taattgtgta ttaaaggata
     4321 ctatttcaat ataatgtaac tgaagttgca ttacagttat aatttttatc agacctttgc
     4381 ctccacaatt aaaattctaa aataaggtgg tggaagtttt tggatatgcc tgtgcaatac
     4441 ttatgggctt gtcgttgggg ctcattggtg gtggcggcag catattaaca gtgcctatat
     4501 tggtttatct gttttccatt gatgcagtat tggcaacagg atattctctg tttattgttg
     4561 gctttacaag tttgataggc tcatttagtc atatcagaat gggaaatgtt catctgcgaa
     4621 cagctgtagt ttttggtatt ccatcaatta ttgcagtctt cattaccaga gcatttatag
     4681 tacctgcaat tcctcaggat ttattcaaca ttggaacgct acagataaca aaacctattg
     4741 cactgctgtt gttgtttgct gttatcatgt tgctggcatc atactctatg attcgcagga
     4801 aaaagcaaaa acccgattat gataaagtac aatacaatta tcctttaatt ataattgaag
     4861 gatttgttgt agggacaata acaggtttgg taggtgccgg tggaggcttt ttaatagtac
     4921 ctgcattggt attgttggct catctcccaa tgaaaaaggc aataggatct tcactgttga
     4981 ttattgctac taattcttta ttaggcttta gtgttagtct gttccatagt gactccatag
     5041 actggcattt gttgttgact ttttctgcta ttgccatttt aggtattata atcggaagtc
     5101 tgatgtcgaa aaaaatatca aacgaaaaat tgaaacctgc atttggatgg tttgtattaa
     5161 taatgggtat ttatattatt cttaaagaaa ctttgttgaa ataataaaac cctttcactt
     5221 caatttgtta ttgcatttca ttgttcttta cactaaactt tattacaaca attattactt
     5281 aacactaaat ttattttgaa ctatgcttga attaattctt aaaccctggc cctggtatgt
     5341 tgcaggacca ctaataggac tcattgtgcc tgcattactg atacttggaa acaagtcgtt
     5401 tggtataagt tcttcctttc gtcatgtttg tgctgcttgt gttccatcaa atattccatt
     5461 ttttaaatat gattggaaaa aagaaagatg gaatttgttt tttgttgccg gcataatggc
     5521 tggtggattt atagctgtga acttactcac cgcatctacc actgttacta tcaattcttc
     5581 attggcaaca tcgcttcatg aaaaaggtat aactgatttt agtggtttgg ttccggcaga
     5641 tttatttaac tggaatagct tgttcacgct tagaggtttt attatgatgg ttgtaggagg
     5701 atttttagtt ggctttggca cccgttatgc aggtggctgc accagcggtc atgctattat
     5761 gggattatca acgcttcaat tgccatctct gattgctacc tgttgtttta tggccggagg
     5821 tttcattatg gctaatttaa ttttacctta tatactttca ctctgaaatt aattttatga
     5881 aaacagtagt tactttagaa aaagatcgaa acacagattt tgaaacacgt tcgttagata
     5941 caatttgtgt aaacgaaagc catcttgaac atagttgggt acacaactta aaatacagcc
     6001 ttatgggaat tttatttgga attattttag taaaggcaga agtgatatcg tggttcagga
     6061 tacaggaaat gttcaggctt caatcattcc acatgtatgg tatcattgga agtgctgttg
     6121 ctgttggagc attgtctgtt tggcttattc gtaaattcaa cataaaaaca ttttatggag
     6181 aagaaatcac cttacatcca aaagaattta ataagggaca aatttggggt ggcctatttt
     6241 tcggattggg atgggctatt actggtgcat gtcccggacc tctgtttgca acaattggtt
     6301 ccggtgctac tgtaattgtt gtgagtttac taagtgcaat tgccggaaca tgggtgtatg
     6361 gtaaattccg tgataaactt ccacattaat cagaagcgtt tcttgaactc tgatattaga
     6421 tttattttag tataattatt taaacaaatt tgtttttgtt ctgttaacca ttggttgtta
     6481 aaccaatgac tgtttaataa ttgtaatttt ttgcaataag atgattagtg tttctgaaat
     6541 tattatccgg ctttcattag catctttttt tggtgcactt ataggattag aaagagagcg
     6601 aaagcattgg gcagcaggtt tacgtacaca catgcttgtt tgtgtaggtt cctgtttgat
     6661 gatgattgta tctgcatttg ggttttctga catcatagga actcctgata taacacttga
     6721 cccttcgcgt attgcagcac aggttgtaac cggcatagga tttataggtg caggtgctat
     6781 tctgttttca aacaataaaa ctattcgggg attaactact gcagcaggaa tatggactgt
     6841 agcgggtatt ggcttagcaa caggaggcgg aatgtatctt gcggctgctg tagcaacagc
     6901 tattgccatt attattttat ggggacttca gcctttagag aaaatgtatt ttgaaaaatt
     6961 caaacaaaaa agtttacgta taaccgcaag tcatgatttt ggtaatacag aatttctgac
     7021 agcactctta agagaaaatg ggatagcctt gatttcatta aatttcacaa gaacaaacga
     7081 agatttgatt tttcaaattc agctcgaaca tgctgataac ggaacaatag agtctgttat
     7141 taatattctc aaaaaaaatt cagccattaa agaaattcaa tggctaaact aatagttgaa
     7201 ggcttttttc tttcttagtt taaataaaaa caatagttct gttttcttgt ttatgtctgt
     7261 caatacttct tttgaagtga tgatttaagt cagctactga tttataattt aattctaatt
     7321 tagcatttaa agtttaataa aaatcttgta atcatgatta acatagggat aaataaaaaa
     7381 caggctaagc aacttgcagc aaaacttaat cttctgcttg cagattacat ggtcttttat
     7441 cagacaactc gtggattaca ttggaatatc agaggtgaga aattttttga actgcatttg
     7501 aagtttgaag aattgtacaa taatctgtta gttaaagttg acgaaatcgc agaacgtatt
     7561 ttgactttag atgaaacacc gttacacacc tttagcgact acaataaggc atcacaactg
     7621 aaagaaatta aaaatgtatc agacccttca aagtcaataa aagcaatttt agatgcattc
     7681 gaaactatat taatcaaaca gcgtgatatt ttgaaaatgt cggcagatgc agatgatgaa
     7741 ggtaccaatg cattgatgag tgattatata agcgagcagg aaaaattagt ctggatgtat
     7801 agcgcatatt ataataaata gaatggaaac aacaacacaa aacagaacat ttaatgacat
     7861 catcaatggc aataagccgg tattggttga tttttttgcc gactggtgcg gaccatgtaa
     7921 aatgatggcc cccatactgc atgaagtaaa aaatgaagta ggcgaaaaag ctgtgatact
     7981 taaaattgat gtggataaaa atcctgctgt tgcagcagct tatgagatac aaggagtacc
     8041 tacattaatc ttgtttaaat ccggaaaagt tttgtggcgg cagagtggtg ttgttaacaa
     8101 accacaactt ctgcgaatca ttgaacaaaa cctgggatag tttttttatg gaattacttt
     8161 gtaggctaat tccataaaca caggacaatt actactcctt tggataatat accgtaaaaa
     8221 ctctgttgag gttaaaacca aaatgaatgc cgccatcaag ccagtcgcct gttgtttcgg
     8281 caataaatcc tttttcaacc atactacgcg aattggtaaa atgtatctga aaaacgtgac
     8341 ctccggtttc aatatcaaag cctacggcaa gtgagttttt atatgtatca ctaatctggt
     8401 caggaaatac ataataatac tctgctgtaa atgctatact cttagaaatt tttattcttc
     8461 cgccaccgcc taatgcaaat acatcatttt tttcatttgc tgttggtacc agattgcgat
     8521 gaatgagtgt tggcattaac tgtatagaaa acgcattgga aagctttgat gctgctagta
     8581 actgatagga ataagtcagc ctggaagaaa aataattttt acgctgtggg tctgtaaatt
     8641 ttaatgagtt tagttgcatt gacgccacag cggtaagtgt aacaggtgtt ccacctttgc
     8701 gttgcttcag aaatttaact tttaataacc catcatacat tttttcaaac gtgctgcgcc
     8761 ctataccaac attcaaccag tctgtaactc catagtcaaa tcccaggcgc atggtggcat
     8821 tgtccaaccc gaaaaactca tatcctccac tatttatctg cccaaagcga tgcaatatca
     8881 ttaactgtaa cactccttgt ttgttatttt caacactatg tccgttaata agcctggttg
     8941 atttaaatgt tgccgtaact tcttctttct tttctttctc tccactttca ctaagcattt
     9001 tcatcaggtc atcctgtgca gttgcatgta gtgtgataaa agtaaatcca ataaaaaaca
     9061 gttttctgat tggtagcata aaaatatatt tagttcttct tttgataagg agcaagttca
     9121 aatttacaag ttactttaac aacttcggca atattttgaa ttactaattt cggaatttca
     9181 acattatggt cttttaaagc aacattaaag ctgccttcaa ttgctggcac gcctccttta
     9241 aaactatagt ttgctttata gtttgcaggc ttctcaatgc catgaatagt aatgtttccg
     9301 tttacagtca cctgatatgc tgtgtcacga tcaattacca attgctcctg aatttttcct
     9361 ttaaaagtgg cgtctttata tttatcactt tccagatatt tttcattgaa gtgttcttcc
     9421 attaatgctt tttcaaattt gaatgaacga atggctacca gcgaaacaat atcacctgtt
     9481 gtaatattca aaatgcaatg tgcctttttg cttgtggcat caatgttttc gagcggtgct
     9541 tctgaaaaga aagaaattgt accggcattg gttgtaaaca cattttgtgc ctgtgcataa
     9601 tgcatgcctg ttattaatac acatagaata aattttttca ttttttaatg tttaattatt
     9661 gggcgcaccc tgattaatcc agattgaaat ttttttgatg ttacagtcac ttaatttatt
     9721 ttgatttttt ggcataggtt catatcccga atcatgattg agtgatccta aaagttttcc
     9781 attgtcggca tagattttaa tcgctgcata agtatcaata acaacattac cctgcgccat
     9841 agatgccgaa tgacaaatct tacaattgct gcttactatt ggtgccactg tctgtgaata
     9901 agttatgtta agtgtatcac agttgcctgt gtttggatat aactcatctt ccacatcata
     9961 ataacaacta ctaatgcaga gtgcaataaa aatacccgaa ataagaatta aacttttatt
    10021 catgctttgt taattgtcgg ggtaaccatc gttaacccat ttaatgattt gttttatttt
    10081 acagttaggg atatattttg ttgaatcttc cttgtcaggc atggtaaaat atccgtctgc
    10141 atgggtaaca gcaccaacta actgccctgt cattgcaaca gcagatacat catcataagt
    10201 aaccaatgaa agatttccca atggtgcact tccgctatga caagtctgac aaatggtttg
    10261 cataataggt aaaatgtttc ctgcaaatgt tacgttaaca ctatcgcaac ccagacattc
    10321 attatttttt gctccttgca gtatccattg gtaaatatcg ttaatctgaa cactgcttag
    10381 tggtgaaccc ggtggtggtg gcatgatatc atcaggatca ttttcaacaa gcactttgta
    10441 gagtttgctg tcttcgggtt ttccggcttt cacttttcct gtctgaataa tattggcata
    10501 agtatctaaa ataacaccat cttctgcagt agtaacatca tgacatccac tataggcaca
    10561 agatgcattt attataggct gaatggtgtt tttaaaataa atagtatcgg agttgcaatc
    10621 ttcagtggca gtgctgtcat ccgttgcaat agccaggtcc tgtggctcgt gcttacatga
    10681 agttgcggca attgtcagaa gcattaccca gaaaaatatt attttattca tgcatcattt
    10741 aaatttaaaa caaataaaaa agttatattc taattatagc ttataaaaat gtgttaatgg
    10801 gaaaatttat cgcctgaaag gtaataaaca caaatattca ttgtttcaac atgcgctttt
    10861 gagttacttt tgctcaaatt ttaagatatg aacggcatga ttttattttt agataatctt
    10921 ggtggcggtg aattgttggt aatcatggtg tttgtattgt tcttcttcgg atcaaaaaaa
    10981 gttcctgact tagccagagg gcttggtcgt gccagccgcg agtttaaaga tgctatgaat
    11041 ggtgtacagc gcgaaataga gcagagtatg caaactccgc caccgccacc acaaaaatct
    11101 gttgaaaagc ctgtaacccc cgaaaaaaat gggggggtag atgaaaaaac cgactaattt
    11161 taggtaaagt accctatttt ttaggggggt gtttgaaaaa aaacgatatt tttcttgaca
    11221 ttgccctttt tttcaatgta tttttgaccc gtcttaaaaa aatccaatca ctatatgagc
    11281 aatgttcgct ttcgtgccct tgatgctacc aatgggcgca tacctgtaaa aattacccct
    11341 ccgtcagtta aggtatctga ctattttgct acaaatgtat tcactaaaga agctatgttt
    11401 aagttcttac ccaagaatgc atttaatgct gtaatgtcag caattgaaac gggtgaaaaa
    11461 atagaccgta aaatagcagg tgaaattgcc catggcatga aagaatgggc catcagcaaa
    11521 ggtgctacac actacacaca ttggtttcaa ccactaacag gtgctactgc cgaaaaacac
    11581 gatagctttt tcgacatcag taatgataat tcattcgagc atttttcagg aagttcttta
    11641 gtacaacagg agcctgatgc aagtagtttt ccaagtggcg gaataagaaa tacttttgaa
    11701 gctcgtggct acaccgcatg ggatccgggt tctccggcat ttattattga aagccctaat
    11761 ggacgcacac tttgtattcc tactatttat gtttcttata caggtgaaac actcgactac
    11821 aaagctccat tgttaaaagc attgcataca ctgaatgaag tagctgttcc ggtggcacaa
    11881 tattttgata aagacatcac tcgtgttagt acaacattgg gtattgaaca agaatatttt
    11941 ttagttgaca cttctttgtt ttatgcacga cccgatttag ccattacagg aagaactgtg
    12001 tttggtcatt caccggcaaa aggacaacaa ttggaagatc attactttgg ttctattccc
    12061 gacagagtaa atgcatttat gaacgatttt gagattgaag catacaagct tggtattcca
    12121 ttgaagacac gtcataatga agtggcccca tgtcagtttg aatgtgctcc tatttttgaa
    12181 gaaataaatc ttgctgttga tcacaatcaa cttctcatgg atttgatgga taaagttgcc
    12241 cgcagacatc attttaaagt gttgtttcat gaaaagccct atgcaggaat taacggtagc
    12301 ggcaagcaca acaactggag tatggttacc aacaccggaa aaaatttatt gtcgccaggt
    12361 ggaaatccaa aatccaacct catgttttta actttcttta tcaataccat aaaggctgtt
    12421 cacgaacatg cagacctgtt gcgtgcaagc attgcttcgg cagctaatga ccatcgttta
    12481 ggtgctaatg aagcaccacc ggccatcatg agtgtttttc ttggtgcaca gttaagcagt
    12541 atcttagata acatcgagaa aaaagttggt tcaagcaaaa tgagtgttga ggataagaca
    12601 gcactaaaac ttaacatagg taaaatacct gaaattttac ttgacaatac agatcgtaac
    12661 cgcacatcgc cctttgcctt tacaggaaat aaatttgagt ttcgtgcagt aggttcatct
    12721 gccaattgca gtaattcaat ggctattctt aataccattg ttgctgatca acttcgtaaa
    12781 ttcagaaaag aagtagatgc tttaattaaa aagaatgtca agaaagacga agctattttg
    12841 cgtgtgctgc gtcagtatat tgttgaaaca aaaagtattc gattcgaagg aaacggatat
    12901 agtgaagagt gggtaaaaga agctaaaaaa agaggtcttt ctaatcatca gacaacacct
    12961 gttgcattag atgcaatgat ttcaaaaaaa tctttgaaac tttttgaaga caaccatatt
    13021 tttacacatc gcgaagctga agcaagacac gaaattatgc ttgaaacata tatcaagaaa
    13081 atagaaattg aaagtcgtgt cattggtgat ctggcacaaa accatattat tcctgccgca
    13141 ttccgttatc agaattttct ggcagagact gttgataatc tgaaagactg cgatctgaaa
    13201 gcagaaacat attccacaca gttgcaactg ataaaagaaa tcagtgctca tgtaaatgcc
    13261 attaagaaaa atgttgatgc catgcttgca tcacgggata aaggaaataa aatagcagat
    13321 ataagggata aagcaatttt ttattgtgat aaggttaaag tattctttac aacaatccgc
    13381 gaacatgttg atgcattgga aatgattatt gatgatgaaa tctggccaat ggtaaaatac
    13441 cgcgaattgg ttacaatcag ataataatgt tgcataaaaa agctgtcctt cggggcagct
    13501 ttttttgttt agaagctata tgcaaacgta agtgcaattg gtttaagatg caacaattga
    13561 tattatttca aaactactta accaaaattt ggaattctaa acttcccaac tcattacttc
    13621 ttataaaaat tcatttcatc aatgtaatca tacacagact cggggagata aaagcgaaca
    13681 tcttttcctt ctttgatggc ttgtctgata aatgtagatg atatttcaat taatggtgcc
    13741 tgaataattt ttactgaagg atggttcttt aattcaccac catcagaatc cggtcgtgga
    13801 taaatgtata ttttatagtt ttctaaaatc tgctcaaagt ttttccactt atgaaaagta
    13861 acaaggttgt cgctgcccat tatcaacaca aactcatgtt gtggaaattt ttcattcaga
    13921 tgaactaatg tattaattgt ataggaaggt ttgggtaaac caaattctat tttagaaatt
    13981 tttattttct tatagctgcc aatggctgct tccactaaat gtaatctctg ataatcatta
    14041 agcaatgagc ccgcaggttt tagcggatta tgtggtgaaa ccaccagcca cacctgttct
    14101 aaatctgtaa actctgcaaa ataattggca atcatcatgt gcccgttatg tacaggatta
    14161 aaagaaccaa aaaataaacc aaccttcatt tagttaattc ttattctgtc aatgcgggta
    14221 attttatctg cctgataatt gatagttaaa atcaacactc ctttatcatc ataaattttc
    14281 cataatccat tccgctgacc ttgtttgtac tgtcgcactt catgcaactt tccattatcg
    14341 tggtaataaa tatgctcacc atcttctagt ccatcaatat attttcctgt aaatgcagtt
    14401 ttgccgtttt tataggtctg atgccatgta ccgaatttta atccatcggc atattcacct
    14461 tcggcaatat attcgccatt gttatatttc caaagtcctt cttttttacc ttcaacatat
    14521 tttcctgttg caatgagttg taatgagtcg ttatactctt tagaaacacc ttcttcctta
    14581 ccattaataa aagactcatc acgtctgatt tcaccattct taaaatacca tttccattca
    14641 ccttcaggaa gtccgttttt ataatttcca atttgctgca ctgctccatt tggaaataag
    14701 aatttccact catcaatacg ttttccatct ttatattttc caatggaatg caccgaacca
    14761 tcatcataga aaaatttcca ttctccctgt ctgtttccat tatcatctgt tattccttcg
    14821 gcaattggtt tatcgtcttc aaacaattta cctgatataa catttccttt ctcatcataa
    14881 attttttcta caccctggcg tttatcattt attacattta caatagactt tacttttcct
    14941 cctaaataaa aatctcgctt tacttcaaac tttgttgtac tttttttatc aattaccagc
    15001 tcgtcatttt tatacctttc tgaaagactt aatgaaccat cattattgta aactctataa
    15061 aaaccattct tttttccatc aagatattct gtttcttctt ttactttatc attatcaaaa
    15121 aaaactttcc atactccttg ctttaatcca aatttatcaa ttctgttaat tctctcttcg
    15181 ctgcgaacat aaccctcgtg ataagttgtg atggtaatta atctgccatc ttctgcataa
    15241 gacttagcaa ttccatcttc aacaccattc ttgaaaggta caatatttac aaccttacca
    15301 ttttcaccaa agtcttttac ccaaccttgt ctgatacctt cttttacttc ttcttcctgt
    15361 tttaacttgc catcggaaaa atagtaacgc tgtaatccgt tttttaatcc gttattataa
    15421 gttaattcac tggcaatttt tccgttttca ttgtaaaatt tccataaacc ttcgggctta
    15481 ttattatgtc tgctgccttc ggattttaac tttccatttt caaaatatga tttccataaa
    15541 ccctcgggct tgccatttac gaagttacct tcactgcttt tattaccatt atgataataa
    15601 aaagttttaa atccattagt aacggtactg tctgtttgtg caaataagtt tgaattaaaa
    15661 aatataaaaa atgctgcaat cagtattcta caaaccatgt tgttttgata tttcaagcat
    15721 gcgttttaaa ggattaagtg ctgcctgctg caaatgttta gggataatta cttccggtaa
    15781 ttcgtttttc atacagagat aaactttctc taaagtattt aatttcatat atggacaatc
    15841 gttacatgcg cagttatttt ccggtggtgc aggaataaat attttttctg gcgacagttt
    15901 ttgcatctga tataaaattc ctgattctgt agctacaata aatgctttgg caggattgtc
    15961 ttttgtatat tttaaaagtc ctgttgtgct gccaataaaa tctgcatgac gtaaaacatg
    16021 gtcttcacat tcaggatgtg caatgactaa tgcttcggga taacgctctt taagttgaat
    16081 aattttttgt tctgaaaata tttcatgcac tacacaacta ccatcccaca acaccatgtc
    16141 gcgaccaatt tttttattga tgtaggctcc aagattcttg tctggagcaa aaataatttt
    16201 ttgatctggc ggaaaacttt caacaatctt aaccacatta ctcgaagtgc aaattacatc
    16261 acttaaagct ttaatttctg cagtgcaatt gatgtatgat attacaacat gaccgggatg
    16321 ttgctcttta aattttttaa agtcagcagg tgggcaactg ttggctaatg agcaaccggc
    16381 attcaaatcc ggcaaaacaa cttttttatc gggattcagc aactttgccg tctcagccat
    16441 aaaatgtaca cctgcaaaaa ctatcattga ggcattggtc ttttcagctt gttgcgccag
    16501 acctaagcta tcaccaacaa aatcggccac atcctgaatg tcggactcct gatagtaatg
    16561 cgccagtatg acggcatttt tttctttttt taatttttct atttcttcaa acaaatccat
    16621 tgaggcagga atactttcag gcaaatatcc atgtttcaaa atcatttcag aaatttcagt
    16681 cattgtaatc aataattata attatataaa tatattaatt tttaaaaata ttatgttgtt
    16741 ataataagtc ggttaatatg tggtataact tttctgtgat gttcttgtat attaaattac
    16801 tgtgcttata ccaacagttt attaactaaa tatagtaact aacattatga ttaataaagg
    16861 cataaatata actattaaca acggttaata agcaaatatg tttataaaaa tcggttaatt
    16921 aagtttgtat tgctgtgaat gactatgttt aaactttggc taaaactcaa ggttaaaaaa
    16981 ttttgatttg atattaaata taatatgaca ctttagatat aaacattaca aacaaaagaa
    17041 ataataatct attaacagaa aatataaata taaacaggaa tgttgttaat tttacaccat
    17101 tataaaatac tattaatcag cactaaaata tttaactgtt aatagcagtt aataacttaa
    17161 atttatcaca tgcagagtat aattataggc agcgatcatg ccggcttcac tttgaaagaa
    17221 gcaataagga agagattttc atcaaatttt gaatttacag ataaaggcac attcagttca
    17281 gattctgttg attatcctga ctatgcacat gcagttgcag gagaggttga agcgggaaaa
    17341 tttacatttg gaatactttt atgtggaagt gcaaatggcg tagctattac agcgaataga
    17401 cataatggtg taagagctgc aatttgctgg atgccagaaa ttgcagaatt agccagaaag
    17461 cataataatg ccaatattat ttgtattcct gcacggttcg ttagtgatga aacagctttt
    17521 ttaataattg agaagttttt tagtactgaa tttgaaggag gtcgtcatca gaagagaata
    17581 gataaaataa actgtgttta aaatagaaaa cagattgcta tgaaattaag atttattctg
    17641 cttatatgtg taataccatt ttttgttaaa gcacaggacg aacaagattc atcagcagta
    17701 acgactaaga aacagaagca tgaaaatcct cgttttgcaa ttgcagcttc aggtggtctg
    17761 agttggagat tagccgatgt aagccctgat gagaatgatt taactaaagc atatattaaa
    17821 gatttaaaaa gcggaaacgg ctttgaaatt tctgcaaagt atttttttaa ttcaaagctt
    17881 ggtgttggaa taaagtacaa cagtcatctt tcaaaggtca aatacgataa tatgtacttc
    17941 tcctataaca atcagaatta ttttggtact gtttctgatg atatttcaat taatactata
    18001 gcagcaacat tcagcggaag attttatacc agacaaaaaa caggttactt tacttttgat
    18061 ggcggaattg gtttaatgac ttataaagat gaatctaaaa ttgccaccaa taaagttaat
    18121 gttaaaggaa acactttagg ctttaatgtt tctgcaggtg tgggtgtaaa tattacaaga
    18181 aatataagtt tagtaattgg ttttaatttt cttaatggaa ccttatcgca atatgatgta
    18241 acagaaaatg gaataaaaga acgcgttaca ttaaatgaaa gcgaatatca aagccttagc
    18301 catatagatg tttttgaagg ccttgtaatt accttttaga tttataaatc tttttttctg
    18361 aatttgtgta cagcaatcaa agccggtaca attatccata ttaaaagtac tatgagaata
    18421 gtaatggttc ctttttgaga acctaaaaac tcattaaaca aagcacctgt ataacccata
    18481 agtgctgcat agtcggtatg aataagtatg agtattctgg ctaagtcaac cggattaaaa
    18541 gtagttagta tcatgagtgg tttttctata ggataatcac caaactgaaa cattaacagc
    18601 agcagtaaac catcataaag aatggtaaaa aacaaccagg caaaaattgc aacaccaatt
    18661 cctttagctt tatcttttgt aatgatggaa ccaagaaatg ctaatgaagt aaatatcaat
    18721 gtaagtagaa tgccggctaa gagaaaaatg agtcctgttg catccggtgt aaaaattaat
    18781 ataggcaaac caattccaat taaaaatgcc aatgttaaag aaagaacaac acctgcataa
    18841 atacttgtta tgagtttttt tcttgagacc ggttgtgccg taagtagttc aataaattct
    18901 gatgaattaa aaatatagat tgtagcataa atcaggcaaa ccaaaggaac aataaagaga
    18961 ataatattta ataaactaat taaacctttt gaagtatttt catcgagatt aaaaatgctg
    19021 aaccctgtaa ccattaaaaa aactagataa gcaattacga ccttgctttt cagaatgtcg
    19081 agcatgatat atttagctat cttattcatg attttttttt agaatggttg taattacttt
    19141 agaaatagat ttctcaccgg tttggagttg cagctcttct aaatttttat gaaacatcaa
    19201 ttcaccttct ctgaaataga caatttctga aaccaactca tccagttcac taagaatgtg
    19261 tgatgtaata attatcagtt taccattatt tttttctgtt ttgacttttt ctttaagtat
    19321 ctctgatgat aaggggtcga gtccggcagt aggttcatca agaataatta ttttaggacg
    19381 aaacagaaag gcaaggcagg cactcacttt ttgccttgtt ccaccggata aagttcccat
    19441 gcgtttgtga aaaacagatt tcatgttaag cgcatcgagt aagtcagtgt ctgtatgatt
    19501 catttgctct ttacgcatat ctataatcat ttcaaacacc tggccaatag tcatattttc
    19561 gggataacga cctatctgcg gcatatatcc aatctgactg cgatagctgt cgtcattctt
    19621 aatatttgtg ttgttaactt ttattgttcc ggattcgggc aacaccattc ctaaaacaga
    19681 tttaataaat gttgttttac ctgaagcatt aggacccagt aacgcaacag cgctgttgtt
    19741 ttcaaacttt atggatatat cttttaatac cctgagtttt ccaaaagttt tgttgatgtt
    19801 tattgattct atcacagtgc tacaggtttc attaatggtt tattatcaac taaattttcc
    19861 ggtgtaatac ttggaataat cttttcgctg cggtcaagta aaaaaacaat taaacttctt
    19921 ataaatattg aagcaattgg aatattctct gtaaccatcg aaaacaaact tacaggtctg
    19981 tagggtacat ctccaatacc atcgcgtttc aaatcataac cttcatattt atcccagtag
    20041 ttgccatcaa agttgtttaa aaccaatgag ccattggttg cgacatcaaa tgtgttgcca
    20101 ttaaagttat tgtcatgaac agtgttatta ctacagcttg cctgaacacg caatgcatat
    20161 ccatttttat tgaaactgtt ttttgtgata tgaagcctgt ttgttccctc catcaaaaca
    20221 ccggtagtat tgttttcaaa caagttatct ttaatatctc catcagaaat ttcttttaac
    20281 aacaaaccat aggctgaagc tcctctgttc atactaaata tattacgata cattgaaata
    20341 tgattggaaa acatgacagc aacaccggca ccattatcag taaatttatt gtcaatgtat
    20401 gaatctttat ttgagaacat aaagtgcaaa ccatagcgca agttattata actgttattt
    20461 tgttcaatat acgagtttcc aacaaattca aaatatatac catctctatg gcctgaaatg
    20521 tcattgtttt ttattgatgc gtacaggcat ttccaaagat gaatagcatt gccggtagat
    20581 tgttcatcag ttcccggtgt tccacgaatg gtattatttt ctattctgaa attatcgcaa
    20641 ttggcaatgt gtattgcaaa ataggagtta atgagtgtat tgtttttaat taaaaactgc
    20701 ctgctgttca tgactttaat tccggctaag tctttcatgg aagaataacc tgaattttta
    20761 attgttagcc cggaaactaa aacactgttt gcattaatgg ttaatacctc aaaattattt
    20821 tctccgtcaa gaactggatt ttcaacacca atcagtgaca gtgatttttc tatgagaatg
    20881 ttttgttctt tataaagacc ggaatggata taaagcgtat caaaaggttg tgctttaaca
    20941 acaccttctt taatagactt aattgaaaag ttcttcccga tttttaaaat tcctgcttgc
    21001 gatttgcctg aaaaaaacag aagaatcaga aaacagctag caacaacaga cagtgtttta
    21061 tttgatttca ttctgaacaa aattccagtc aaccaatgaa ccaccatcat ctttcaatac
    21121 tttttcggca tcttctttat tggcaaaagc agcaaagccg ctattcattg gcgtacgaag
    21181 gttgtcgctt tttaaataga cggcatcagc tgcattcatc aaatcaatct gatcaaaata
    21241 atttacaaca tataattttg cagagcctgc atctacgtta gatgattgtc tgctccattg
    21301 caacatacag ttaatatcat caaacttata aatacgacct ttgtttgata ttatttctgc
    21361 accaaaacga gtgtctgcaa caggcatctt gcaatgtaca catgcatctt taccggcaac
    21421 taatggctca ggctttgttg tacatgatac tattagaata gaaattaaaa tgaacgtcca
    21481 cctcatacac tttatttttt acgatttgct ttttcttaac cccattgccc agataatagc
    21541 acagataagt gcagagccaa ttacaatata acctcctaca tctggcatag agtgagcatc
    21601 aaagttgagt agctgtttat gtcctaatat aggcggttga taaaccatgc caggcacttt
    21661 aatgggtgca ttaggatcaa gattatgtcc ataatcataa ccccattgat agaaatcgta
    21721 gagagcaagt atgccacaaa taacagaaac taccaggtaa ctaaacaata ttttacgact
    21781 accggtaatt gctatcacta atccataaac aatgaatgca gctacaatat atttcaagaa
    21841 ttcaaattca ggaaacatct ctacgctgat ttttttcatg cctatgtaat ggttcaaacc
    21901 attaataacc tctacgtcac ctgttaattt atcgagccat attttcataa ccaagccttc
    21961 cggatattgc ggagcgaata aatcaattcg ccagatgggt acaaaaaaag aaatagctat
    22021 aagtaaagaa gagattgcaa tgataactct tgatacaccg tttatttttg tcataaaaat
    22081 ttatatttaa ttttacctcc taagaattaa attatttatc aggtggcaat aaaacggcat
    22141 caataacatg tatcactcca ttagctgcag gaatggtagc aatgatattt gcctgattgt
    22201 taatcatgat tttattgtcc ttgtttgaaa tagcaatgtt tttcaaattt gcctgattga
    22261 ttgtttggcc atctgtaagc atttccggtt tgaaaacacc aagtgaaacg tgatattcaa
    22321 gaatatcctg aagtttggct ttattttccg gtttcattaa atcatctaca gtacctgcag
    22381 gtaatttatt aaacgcttca ttagttggtg caaaaacagt aaatggccct gcatttgaaa
    22441 gagaagtcac atagtcggct tgcttaagtg cagctactaa agtagaatgg tctgctgagc
    22501 ctactgcaat cttaacaata tctttttgtg aatcatcgtc aataacagct tcctgtccac
    22561 ctgcctgtgt ttcttcagtg gttgttgctt gttggttttc tgctgcggga ggattacagc
    22621 taattgctat tgatgcagca aaaattactc ccattaataa ttttgtgttc ataataatct
    22681 ttagttttat aatgcacctc gttggtagtg tcacaacgag gtgcgttgtg taatgtttat
    22741 taaattaaag atttagttga tagggggttg attagtacct gtgctgaaag ttaatggtac
    22801 gttagaatta gccggactga ttctgatgta gccttgcatt tcctgatgta atgcagaaca
    22861 gaagtctgta caatagaatg ggaatacacc tactctatcc ggtttccaca ttaaggttga
    22921 tgtttctcca ggcatgatta ataattcagc attattagca ccttttacag caaagccgtg
    22981 tggaacatcc cagtcttgct ctaagttggt tacgtggaag taaaccacat cacccaactt
    23041 aacaccttca atattatcag gaacaaagtg tgaacggata gatgacatat aaacgtgtac
    23101 ttcgtttcct tttctttcaa cttttgcttc tttttcgcct ttcgttacat aaggatgttg
    23161 attctcttca atcttataaa tttttgtaga gttttttgat agtaattctg caggaatagc
    23221 ttctgcatag tgtggctcac caattgttgg gaagtctaaa ataagttgca tcttatcgcc
    23281 actgatgtca taaagttgtg cactttgtgt taactcagga ccagttggca gataacggtc
    23341 ttttgtaatt ttattataag caattacata tttaccccaa ggcttttttg ttggaccacc
    23401 gggaacacat aagtgaccaa cagaataata agtaggcaca cggtcaagta ctgatagatc
    23461 actcaaacgc cattttacaa tctctgaaga tacaaagaat gaagtgtaag catttccatt
    23521 tgcatcaaac tctgtgtgta atggtccaag accaggtttt ttaacttcac catgtaacac
    23581 cgcatcatat tttaaaaccg gtaagccatc ataatcaccc tcaaagttct tagcgttaat
    23641 tgcttcctgc atttttgtaa aagagaatac aggaataaca gcagccaact taccgctacc
    23701 aacaatatat tcacctgttg gatcggtatc acaaccgtgt ggtgattttg gacaaggcat
    23761 taaataaacc atatcaggac aatcttttgg atcgagcatg ataacctctt tttctatagt
    23821 cgaattggct gtgtgtgttt tttcatcata agtattatga gcataagaaa catttttctt
    23881 aacacccttt ccggctttta catattcttc ggctttcttc cagttaacgg ccataataaa
    23941 gtctttgtca aacttcgatg cattaacttc aagtagcgtg tttgctcttt cagaattgta
    24001 acagctgaag aaaaaccaac cgtgagattt acctttacct gcacgagaca agtcaaaatc
    24061 aacacctggt agtaaaattt ggaatgcaat tcccattttt cctgattctt tatctacatt
    24121 gataaagctt acagtacctt taaaattttc tttgtaggta ttaatcggaa cgtctgttgt
    24181 tccattagac atgggtacac taaagcgggt accggcaacc acatactctg aattttcagt
    24241 aataaatggc gaagagtgat ttcctgcact gttaggaatt tcaatgattt ctgctgttct
    24301 gaaagttttc aggtcaatac gtgcaatgcg tggcgtgttg tttgcatttc cgaaagccca
    24361 gcgaccatca tgaacaccat cagtctctga taacgaaata tggtgcaaat catcccatgg
    24421 aacaaatccg tgtgaagtgt taagcatcgc cttggattct tcactgtagc catatccttt
    24481 ttcaggatca acagaaaata caggaattac tctgaatagt cttccagaag gtaatccata
    24541 aatactcatt tgaccactaa aacctcctga tacaatgttg taaaactcat catatttacc
    24601 gggggcaaca taggcctttt ttgctgcatc gccagaagtg gcagtactca catttttagg
    24661 cttgcacgag ggtagtgcag caatagcaat tacagctatt gacgccaata ctacattttt
    24721 aattttcgat tgtttcatta tataatttat ttatagttcc ttattttaca ccatcgttct
    24781 cacgcatata ctcatagata tgacgcgcgt catcatcact caacccttga tttggcatac
    24841 gcaccaaaca ttcctccaac aaacctttag caatagcgtc cttatctaac atttcatcag
    24901 gattggttga aaagttcatg atccactctg cagtgcgttt acctgtaaca cctttccatc
    24961 cgggtcctac taatttctca tcagttagtt tatgacaagc agaacatttt acatcataaa
    25021 tagattttcc ggcatctgcc atttgtttgt caagttttgg gtcaactttt acatcagtaa
    25081 actttccaaa accttttcca tcatctgcaa ctttagcgtc ttctttttgc ttttcttctt
    25141 cgtagtactg attcatatct ttagactcag cagctttttc ttctttagtt gcagatccgc
    25201 cacatgcagc aaaaagggtt atgcccattc cggcaatcat taaattgatt aagtgttttt
    25261 gaattgattt catattgatg attttaatta aaattttacg gaacaaatgt attaataatt
    25321 aacagggtgt tcaaaatatg ttaatatgat tgatgtcaca caaactattg ataattacat
    25381 gtaacacgaa ctgcttttca gcatgttttt ttaaagcgtt taaatacctt aaaaagtaat
    25441 aaggaaaaat taagtttata gtatgctacc cattttgttt agaaaacaat ttagcagaaa
    25501 acagaaatca agcaagtata gagtaatgca acaactatgg ctcccataat aatatataaa
    25561 gctcttaaac ggtctttctt tttacaatgc ctgtcatcaa tagcatcaag atttcccggt
    25621 tcatgaaaat tatggagact aaaaatagca aacagcacac aaaatctgct gacaacttca
    25681 acaaacagaa tgattgttgc taatgttgtt aggaaaagga gataaaaaac ccacaagcta
    25741 tgatagaaat gaacggttat tgttaataat atactaatca gcaaaaagaa aatcagaaat
    25801 ttttgcctta ttctgatctc agcttcacct atgttacagc acccggcttt atactgattt
    25861 gtgtacattg tatcttttat taaactttaa catcaaagcc gcattattgt tctgtaaaaa
    25921 tattaatagt ggtgaaatta atatagactc tctttattta ctttcacagc caaaataggg
    25981 aaagaaaatg atgaaaaaaa cggcagttat tttttttata ttattcagtc agttcataac
    26041 tgtttttggt cagtcgcagg actctttgat acttcgaaaa atttacaatg aatcattaga
    26101 gcacggacag gcttatcatt accttaaaac gttgtgtaag caaatcggac acaggcttag
    26161 tggttcaccg caagcagata aagctgtatt atgggcacaa aaagcgatga cagattttgg
    26221 tgcagacacg gtttttttac aggatgtcat ggtgccgcat tgggtaagag gtgaaaaaga
    26281 gaatgcttct ataatttctt caaaaaacaa tcagtcaata ccattaaatg tgcttgcttt
    26341 aggaggaagt gttggaacag atggaaaaac tctgacagcc aacgttgtgg aggtaagttc
    26401 attagacgaa ttagataaac ttggtgctgc aaaatgctct ggtaaaatcg tttttatcaa
    26461 tcagcgaatg gatgcaacga aaattatgac gtttgatgcc tatggtcctg ctgttgctat
    26521 tcgttgggca ggagctaaaa gagcagcacc cttaggagct gttgctgtaa ttattcgatc
    26581 agtaaatcct tcattagatg attttcctca tacaggtact ttacattacc ccgatacaat
    26641 taaaaaaatt cccgcttgtg caataagtac aaatgatgct gaaaaattat cagctatgat
    26701 taaaagtgat ccgcaactta aaattaagtt gaagatgaac tgtaaacaac ttccggatgc
    26761 accatctcac aatgttatta gtgagattaa aggcacagat gcaaatgccg gatatcttat
    26821 tgttggcgga catcttgact catgggatac aggtgagggt gcgcatgatg atggtgctgg
    26881 agtggtgcaa gcaatggaag tattaaacct gtttaaaaaa gcaggtatta agccacgaca
    26941 cactattagg gctgttgcat ttatgaatga agagaatgga gggaaaggtg gtgagtatta
    27001 tgcaaaaaat gcacttcaaa aaggcgaaaa gcatattgca gctattgaat ctgattgtgg
    27061 aggttttact cccagaggat tttttttaca aggggattca ttgaaaaaag aaaaaataag
    27121 gtcatggaag aatctgtttg aaccttatca gattcacagt tttaattttg atggcggagg
    27181 tgcagatata ggacatttaa aaaaccagaa cacctttttg ttaggtttgt tacctgacac
    27241 acaacgctat tttgatattc accattcgcc aaatgatgta tatgagaatg tcaacaggcg
    27301 cgaattgcag ttaggagctg ctacaatggc tgctatgatt tatttgattg ataaatacgg
    27361 tttggaataa tgttgttgaa agaaacagaa ctgaaacaaa atgcagcagc ttatgctgac
    27421 cagcaaaaga atatttcttc tgtagcacca caaatatttt taaacagaaa gaaagagatt
    27481 ttttctgatt atgaactggc aacccgcagg gcggcatatt atcgttggaa aagtacagaa
    27541 agtctcgaca aattactcat cgcctttgaa gccaatgcaa ttaaagcagg aataaaagtt
    27601 ttatgggctg ttgatgtcgc tcaggcacat ttggaactga atgctgttgt taataaattt
    27661 caacaaagta aaattcatat ccacacgtgc tacaacgaac ttgaaactga aaaattaaat
    27721 caaccttcat ctatcaatac taagaacaaa atcagcattt gtgatgcaat ttttgttgct
    27781 gcagaaccgg caacagttgc ttgtttctct gacaaaaatt tttatgaaag cgatgttaaa
    27841 attttacttg catcggttga caaattgatt tcttctgtaa atgaacttga actgctgatg
    27901 tcgctgcaat cacttcaaaa aagcaattca ggagaatctt tttctttaat tacgagtgct
    27961 ggacataaca gccaacggca ggatgtttat gttttgattt ttgataatgg aaggagtaaa
    28021 ttattagaaa gtcagacaga aagacaaatg cttcattgca ttgattgtgg aatttgtcag
    28081 gtggtatgtc cggtaacaga aagtattggc ataacaggag gcaatgaaaa taattatggc
    28141 ccgccatcat atatcagagc tgtttttgac ggaggacaac gtgaatacgg gcactatgca
    28201 ggattgtgca atatgtgtgg taaatgcaat gtacactgcc cggttcatat tgactttaag
    28261 aactcgtttc tacatctgcg tcatgagaat gtccagcaga aacaccgttc gcaaaaggag
    28321 cggctttttt atatggtttg gagaaaaaca atgcttaagc gtgagtttat gaattggaaa
    28381 acggttaatc cattacgcta tgccgtagaa acactttttt tgaaatctaa aaccggaata
    28441 cgcaaactgc cggagcccgc tacaaaatca tttaatcaga tgtggcgtga taaaatggga
    28501 atgaaataat ttatttcaga atttttttca aatcaggtct tagcctgctt ttgtaaacag
    28561 catgtgcatg ttttttagca cgtcctttac gaagtcgtat ttgttcttct tcaggtaggt
    28621 taataatagc tttacattca ggagtacaac agccttcata agttgtttta catgagtcgc
    28681 actgaataaa taacaagtgg caatcatcat tggcacagtt aacatgtgta tcgcatttgg
    28741 caccacattg atggcatgtg ctaacaatat catcacttat tctctcgccc attctttcat
    28801 caaacacaaa attctttccc agaaatttat tttctaaatg ttcagcattt acttctcttg
    28861 catattgaat gatgccacca taaagttgat ttacatcctt gtaaccatga tgcttaaaat
    28921 aagcacttgc cttttcacaa cgaatgccgc cagtgcagta caataaaatt ttattgtctt
    28981 tattagtctt ggtcatttca attgcttgtg gcagcgcatc acgaaatgta tcagcatcag
    29041 gctttaaagc atctttaaaa aagcccactt cactttcgta gtgatttcgc atatcaataa
    29101 caatggttcc tgactgttgc atagcctcat taaactcttt tgcagagaga tgattaccca
    29161 cattggtaac atcaaatgta ttgtcgttta aaccatcagc aactattttt gattttatgc
    29221 gaacgataag tttgtagaac gacttgccat tatcttcaat ggcaattttg agtggcatac
    29281 ttttaagcaa aggttgtttt tcaagataat ttttaaaaac atcccaatta tgttcaggaa
    29341 cgctgatttg tgcattaatc ccttctgttg caacatacac tcttccgaat atgtttagtg
    29401 cttcgaagtc aatataaaga tgatttctga aatcttcaac atcctcaatt ttaaaatacc
    29461 tgtagaaaga aacagttttt ctattgaatt tttcttcggc aagacgttgt ttgaggagtt
    29521 gcttacttac aaggtttcgt aattgcatgt tacgcgttgt aattaaacag ttagatataa
    29581 taattaaact tctttagttt cttttttctt gattttattg taaaaaaaat aaaagccaac
    29641 accaaccata acatagggaa cagccattaa ataaagtatt cctttattta ggcctttacc
    29701 ggctttgtat ccttgttcaa cactgctttc tgccccggcc ctgcaaacac tgcattgtgc
    29761 atttacattt tgaaaagtaa gtgcagacaa gaatataatt aataaaatta aaatccgttt
    29821 catattatac ggttaatgtt cgtttaacgg caacctgctc atagccttca attatgtcgc
    29881 ctactttaat atcgttgtag ccttctatat tcaaaccaca ctcaaatcct gcagaaactt
    29941 cttttacatc atctttaaaa cgcttgagtg atgcaagttt gccggtatat gctacaatac
    30001 catcacgaat aaccctgatg gcagtatttc ttgtcatttt tccatcaagt accatacagc
    30061 ctgcaatagt tccaaccttt ggtattttaa atgtttcacg cacttcaata ttgcagacaa
    30121 tcttttcttc aaactcaggc gcaagcattc cttccatagc cgttttaatt tcattgatag
    30181 cgtcatagat aatgctgtag agccttattt caatctcttc tttttctgca agtctgcgtg
    30241 catttactga aggtcgtacc tggaagccaa caatgatggc gtttgatgct gatgccaaga
    30301 gaacatcaga ttctgtaatg gcaccaactg atttgtgaat gacattaacc tgcacttctg
    30361 ctgttgaaag tttaagtaat gaatcggcaa gcgcttcaat agaaccatcc acatcacctt
    30421 ttactattac attaagttct ttaaagtttc ctattgccaa acgtcttcct atttcatcaa
    30481 gtgttatatg cttatgtgta cgaatacctt gttcgcgctg caactgcaat cgtttgttgg
    30541 caatttctct ggcttctctt tcatcgtcca tcacattaaa ctgatcaccg gcttgtggcg
    30601 caccattaag accaagaatc aaaaccggtg ttgcaggtcc tgcatggatt cttttatttc
    30661 cacgctcgtt gaacatcgct tttacgcggc cactgtagca tccagcaagt actacatcac
    30721 cagttttcaa tgaaccggat tgaaccaaca cggttgcaat gtatcctcgt cctttatcta
    30781 atgatgattc tactactgtg ccaattgcat ttttatttgg gttggctttt agatcgagca
    30841 actcagcttc tagcaaaact ttttcaagta acaggtcaac attcagtcct tgtttagcac
    30901 taatttcctg tacctggaat ttacctcccc aatcttcaac caatatattc attttagaaa
    30961 gctgctcctt aattttttca ggattagctc ccggcttatc aattttattt attgcaaaaa
    31021 caattggaac gcctgcagcc tgtgcatggt ttattgcctc aatagtttgc ggcattacgc
    31081 tgtcatctgc tgcaattaca attatggcaa catcggtaat ttttgcacca cgggcacgca
    31141 tagctgtaaa tgcttcgtgt cccggtgtat caagaaaagc tattttcttg ccattgtcaa
    31201 gttgtacttc gtaggctcct atgtgctgcg ttattccacc tgcttcgcct gaaataacat
    31261 ttgtttttct gatgtaatct aataaagaag tctttccgtg atctacgtga cccattactg
    31321 ttacaattgg tgcacgtggc ttcaggtctt cgggtttgtc ttcaacgtct ttaatagctt
    31381 cctgtacttc tactgaaacg aattcaacag ttgcaccaaa ctcttcggcc acaacactta
    31441 gtgtttctgc atcaaggcgc tggttgatgg acacaaccat acccagactc atacaggttg
    31501 atataatctg attaataggt acaccaagca acttcgacaa ttcgctggcc gttacaaact
    31561 cagttacctt aattactttc ttttcgttac tttcctgctc aagttgtgat tgtaattctt
    31621 cctgaacttg ttcgcgttta agttttttaa gtttagatga cttggatttt cctccaccac
    31681 tcagtcgtgc taaagtggct ttaatctgat cctgaatttc tttatcagta acttcaggtt
    31741 tagcttctgc cggtttattc cggaaatcat ttctgcgttt aaaatcacca tgtcctccac
    31801 cgggtctctg ccctcctgct gtacgtggtg caccaccctg atgtggttga ttgccagcct
    31861 ttccaatgtc aaccttctta tcttttcttt ttctcttttt ctttctgtca tcttctgatg
    31921 aagtggcaac gggctttttt ttaggcggat caacgggtaa gtttatcttt cctaaaatag
    31981 ttggacctgt caatttttca cgatccgttc tgaataattc cggaggagtt gtatcatctg
    32041 ttttttgttc tgtttcaaca gtaacagttt tgtcagtttc ggcagtaacc ggggttgcct
    32101 cagtgactac ctcaggctgt gcctcaacaa cagcaacagg ttcctccttc gttgttacag
    32161 cagctacttc ttcaatttgt tttacttctt cgtcttcttt tttctttgtg ggtttctttt
    32221 tctttggctc ttcctctttt acttcaactt ctgccttttt ctttgctgta gttttctttg
    32281 gttcttcggc agctatttct tctttcttag ttgttttttt ctttttcttg tctgtacttt
    32341 caagatctat cttaccctga attttaggac cttcaagctt gcttgctttt gctttaacaa
    32401 cttccggctc ttttgttttt tctttagctg caaccggttt ttcaacttcg gctacaggtg
    32461 caggttcttc tgcttttaca gcttttgctt tagttgtttt ctctttggtt tcttttgctt
    32521 tagcagtagc ttgtttttct gttgcctgtt gctttatgct aagtagttta tcagccaatt
    32581 catcatcggt ttctgactca gatactgatt tgcctgaatc atcttttact tcaatgatgg
    32641 tgttttcgcg tttttgtttt tcgcgtgtaa tctcattggc ttcctgacgc gcatgcatat
    32701 caggtgcaaa ctctttcgtc agcagagaaa aagcatcagc actgattttg gtgttagggt
    32761 tcttttccac ttgaattcca tttttattca gaaattcaat aatgtggtca atacccacac
    32821 ccaaatcttt ggtagcttta cttaagcgta cgtctttttt ctcgtctgac attaaacagt
    32881 ctatgttttt atcttaaaaa ttaattacag catgaatatt ggctgcaaat ttagcttatt
    32941 caaactcttc attcagaatt cttacaactt cgcgaatagt ttcttcttca aggtcggtgc
    33001 gttttaccaa atcatcaatg gatatagcaa gcacatcttt agctgagtcg caaccaattt
    33061 tcttgaactc ttcaataatc catggttcaa tttcatcagt aaactcatcc aaatcaacgt
    33121 cttcatcgtc ttcaacatca gaatcacggt aaacatcaat ttcaaatccg gtaagtttac
    33181 cggccaactt aatattatta ccacctttgc ctatggctaa tgaaacctga tcgggtttca
    33241 tataaacagc agcttttttg gtgtcatcat caagctttat ttcagaaatt tttgccgggc
    33301 ttaatgcacg ctgaatgtaa agtgataaat tgtttgtgta atttattaca tctatatttt
    33361 cattgcgtag ttcacgaaca ataccatgaa ttcgcgaacc tttcatacct acacatgcgc
    33421 caacaggatc aattctgtca tcatacgatt caacagcaac cttggcgcgc tcacccggct
    33481 cacgaacaat attttttatt gtaattaagc catcaaacac ttcgggtact tcaagctcaa
    33541 acaaacgctc gaggaatacc ggtgctgtgc gcgataaaat aatttttggg cttccaccct
    33601 gcatttctac ccgaagaact actgctcgca cagtatctcc ttttttaaag aagtcggcag
    33661 gaatctgttc gcttttaggt agtgacaatt cattaccttc atcatccatt accatgattt
    33721 cttttttcca aacctgataa acttcaccgg taacaatttc tcctacgcga tctttatatt
    33781 tctgatagat ggattctttt tcaagctcca taatacgggc aactaaattc tgacgtgcag
    33841 caagcacagc tctgcgacca aagtcatcaa gaagaatctg ttcggtaaca tcttcgccaa
    33901 cttcaaagtc cgattcaatt tttaatgcat cgctcaaagc tatttgggtg ttgtcatctt
    33961 caaccatacc atcttctact atggttcggt ttctgtacat ttccacatca cctttatcgg
    34021 tattgattac aacatctaca ttgttgtctt cgccatattt ttttctaacc atagcacgga
    34081 atacgtcttc catgatactc attaatgtgg cacgatcaat gtttttagag tctttaaact
    34141 cactgaatga ttctataagg acggcatgat ccattgtatt gatttattat ttaaattcta
    34201 atttagtttc tttaatattt tgcatcggca tagtagttgt aatttgcttt tcaacctttt
    34261 taccggctat tttttcttta attgtttcca gcaattgcaa ttcttgttca ttgacatcaa
    34321 caagaattcc tgcatgttgc ttaccatctt taagtaagat ttttaccgga cggcctattc
    34381 gtcttttata ttgaccaaga actttaaaag gttgatccat tcccggtgaa cctacttcaa
    34441 actctttatt ctcaaacaca cctgacgatt ccatctcctt gtatagataa cgattgagtg
    34501 atacacattc atcaatagta atacctgccg gcttatcaac caaaacagtt atttttccgg
    34561 gagtaacttt tatatccacc agaaacaggt ctttgtcagc aagatagacc ttaattaatt
    34621 catgtatttg ttctgcaatc atcttaaacg ggtaatgaat aaaaaagggg acattgtccc
    34681 cttcacttac caaatacgcc acaaaagtat aaaaaataac taaacttgaa atgaaaagta
    34741 tatttttacc attgattata ccttaaacaa aaatactatg gccgacaacc taaatttaga
    34801 aaagatactt ttcttagaca ttgaaactgt tcctcaattt gaatcttatg cacgtttacc
    34861 cggttttgac aaagatttgt gggattataa ggcgctccat ttaaagaagg ttgatactga
    34921 tacagcagag agtttgtatc caagagcagg tatttatgct gagtttggca agatagtatg
    34981 catttctgtt ggttggttta tgactgaaag taacagaaga tattttatag ttaagtcttt
    35041 ttggggtgac gatgaaacag atttgttatt gaaattcagg gagttattaa cgaataatta
    35101 taataaatca tcacatttgt tatgtgcaca taatggtaaa gagtttgatt ttccttactt
    35161 gtgcagacgc atgcttttaa ataaagtaag gattcctgat attttgaata ttggtggtaa
    35221 gaagccatgg gaagtacaac atttagatac tatggacatg tggcgttttg gagattataa
    35281 aagctataca cctctgaaat tactgactca tatatttaat attcccagcc ctaaagatga
    35341 aattgatggc agcgaggtct ggcatgtgta ttggaaagat aaggacttaa gaagaatagt
    35401 aaagtattgt cagaaagatg tagttgctgt tgcgagattg cttcttgcac ttagaaacga
    35461 aggtgaattg aatgattcag aaattgttat tacagactaa ctgttttttc tgaaaatata
    35521 aatcagactt gaataatttc cagtgtaaat agctgcatgc atattggaat ggatacctct
    35581 gtaaagtgcg ttataaattt gcacagcttt tgacgatttg gctttataac gttcacttaa
    35641 taatgaaaca taggtagagt caaatagcat gggtttttta tctagtaatg taaaattaaa
    35701 cgatcctatt aacttttcta atgcttcagg agtaaaatga taaaggtgtc ttggcacatc
    35761 ataagcggcc caaaattctt tataatattc agcatcttta gaagcaggat tgggtactgc
    35821 tatgattaga attccatttt ttttaagcgt tattttgata tggtttaagg tttcttgaag
    35881 tgtatgaata tgctctaata catgccaaag ggttatacag ttaaactctt cattaccagt
    35941 agtataaaat gtttcaatag ggtgtaagtc agtattaaaa gtttcttttg caagtttaat
    36001 tgcctcgagg gatggttcat aacctttaat agggaaaccg gcttttaaaa ccgcttttac
    36061 gaagtatcca tttcctgcac ctaaatcagc aacagaggag tttttaggaa tcagtttttt
    36121 aatcaatcta actttttgat ttacagctat attcctaaca attggatata acctattaat
    36181 aattcctttg cttttattgg tatgggaaac gtagtcatca gacttatagt acgcgcctat
    36241 ctcagacgca tcaggtcttg gcgaagtgaa taacaattta caactaacac attgattaat
    36301 agtgaagtag gatcccgaat aaccaaagtc tttacaggtt aagtattcaa ttttagcatt
    36361 agtcccacaa gcggggcatt cattaagtat ttccatacag gggcaaaaat aaaaaaagac
    36421 caccatcagg tagtctttag tttcacgtga aacgacactt ttattatctt cctaaataaa
    36481 ccattaagat agatatgtca gaaggagaaa caccgcttat ccgggaagct tgtcctaatg
    36541 tttgtggttt aacaacagaa agcttttgtc gtgcttctgc actcaatgat tttattttat
    36601 cataattaaa atcttcgtgc aaaataagat tctctaactt attaagttta accgtgtgtt
    36661 caaactcttt tgcaatgtaa ctttcatatt tcatggctat ttcagcctgc tcaatgactt
    36721 cgttttttaa ttcgggattt atgaatgata atttttcaga taaagaggct gaatgcttta
    36781 taagatcagc taaatttatt cctggacgat ttaaaacctg aatgactttt actttttgtg
    36841 aaagttctgc agactctttt gaattaaggt agctattaat ttctgctggc gaaatacttt
    36901 cagatctgca tgcttcaatt attctgtcca cggcctgttt tttttctgtt acacgtgaaa
    36961 atctttgtgc atcggccaaa ccaatctgat gacttaccgg agtcaacctg atgtctgcat
    37021 tatcttgtct taaaagcgta cgaaattctg cacggctcgt aaacatacga taaggttcat
    37081 cggttccttt agttacaaga tcatcaatca atactccaat ataggcatca gagcgtttta
    37141 aaattaatgg attttgatta tttaatttaa ggtgagcatt tattccagca atcaaaccct
    37201 gacaggcggc ttcttcataa ccggtggttc cgtttatttg acctgcaaaa taaagatgtt
    37261 ctattttttt agtctccaag gtcaggttta attgaagagg agggaagtaa tcatattcta
    37321 ttgcatagcc gggtctgaac atttttacgt tttccagtcc gggtattttt ttaagcgcag
    37381 catattgtac ttcttcgggg aggctggatg aaaatccatt caggtaaatt tctactgtat
    37441 tccatccttc gggttcaata aaaagctgat gacgatcttt atctgcaaag cggtttattt
    37501 tatcttctat agacgggcag tatcttggtc caagtccttg aattctgcct gtaaacatgg
    37561 gcgatttttc gaatcccgtt tttaagatac cgtgcacctc ttcattggta taggttatat
    37621 ggcagctgcg ttgtgcagag agaggttttg tgttagtgaa ggagaattta ccggggtttt
    37681 catcaccctt ttgctcttct aatcttgaat agtctatgct gcgaccatct aagcgcggag
    37741 gtgttcctgt tttcattctg ccgcttacaa aacccaagtt tactaactgc tctgtgatgc
    37801 cagttgcagc tttttctccc gaacggccac cgccaaagcg cttttcaccg atatgaataa
    37861 tgccatttaa aaaggttcca ttggtaagta ctactgcttt gcatcgtacc tgatgtccca
    37921 tgcttgttac aacacctttt agtttgttat cttcaataat taaagcacta accatatcct
    37981 gccagaaatc aacattaggt gtgttttcga gcatttgtcg ccattctgag gcaaataaca
    38041 ttctgtcatt ttgtgtacgt ggactccaca tggcagggcc tttagagcga ttaagtattc
    38101 tgaattgtat agctgatttg tcagaaacta taccggaata gcctcctaaa gcatctattt
    38161 cgcgaacaat ttgtcctttt gcaacaccgc ccatagctgg attacagctc atttgcgcaa
    38221 tggtttgcat gttcattgta atcaataaaa cacgactgcc catattggcc gctgctgctg
    38281 ctgcttcgca gccggcatgg cccgcaccca ccactacgat atcataccct tgaatcatat
    38341 aatagcaata ataaaaacca aaacacttgg tttcacgtga aacttttaaa aactgttgaa
    38401 atatatttgt aacccctttt aaatcaaaag gttcgcaaag ataggtattt gttttctgcc
    38461 tttgtcattt tttctttatc ggcagccttt ttatcactat atccacataa atgaagtaca
    38521 ccgtgaatca tgacacggtg tagctcatct tttatactgt ttttcaatag tttagcattt
    38581 tctctaactc tgtcaatact gataaaaata tcacctgata taatagattt ttctgaataa
    38641 tcaaaggtga taatatctgt taatgttttg tgattcagat atttgatatt cattgagtat
    38701 aaaaactcat cagagcagaa tataaaattt aatgtaccgg gttttttatg gtgcttttta
    38761 atacaatccg ccagccaaga agacacagct tccttaccaa tcaggcgaaa ttgcacatct
    38821 tccttaaaaa aattcacctc aggtttcatc atttagccgg aacaggaggc tccataacag
    38881 ccttgcattg actgcaagtt cttttttcga gtgaattgta gaaatcgttc atcaccacag
    38941 gcaattgatt tacaatatct ttcaatggga aaaactcttc atatagtttg tgattgcatt
    39001 tttcgcaaaa ccacatgcag gcatccattt catcaggctt acgatagcgc tctactacta
    39061 aaccaattgt gtttgcacct ctttgtggac tgtgtggaac acgtggtggt aataagaaca
    39121 tttcaccttg cttgatagga acatcaacag gttttccatc ctgaatggtt tttactacaa
    39181 tatctccttc tatttgataa aacaattctt ctgcatcttc aaaatggtaa tcttttcttg
    39241 agttaggacc accaacaatc atgacaataa aatctttatt gtctatataa acttgtttgt
    39301 tgcctactgg aggttttaac aaatgacggt tttcttcaac ccactgctga agattaaaag
    39361 gtttgctgac tgccataatt ttaaaatttt atttttgaaa tagattgttg agtttcttag
    39421 atacttcttc ggtaaactcc tgtgcttttg tgccaaggtc ttttgccgag ctttcaagat
    39481 catcctttag ggtctcaaat ttttgtttaa caccatcaga taagttacct ccggttagta
    39541 ggatgtctat ttggttatgt aacagatatg gtaatttttc tttcaatgca gctgatacag
    39601 cctctattgc tttggttgct tgctcctgat taatattggc agccttgctt acctggtcaa
    39661 cgattggatt tgccatgatt ttgtgttttt ttgttttcca aagttatgct aaaaaaatgt
    39721 ttatcaaatt aagtcaggta catttgcatt atgaatctgg atttaacgaa taaaaaaacc
    39781 cttgtttgtg gtagcacaca aggcataggt aaggcagcag caaaagaact ttgtcttctt
    39841 ggtgcaaagg tagtgctgtt ggcaagaaat gaacaaaaac ttaaaacagt agctgcggaa
    39901 ttaagcacac ttagtaagtc agtacctgac tatattgtgg ctgatttttc tgctcctgaa
    39961 aatgtaagac agcaggttcg tgaatacata aataaaaatg gcgggttcaa tattctaata
    40021 aacaatgcag gcggtcctcc ggcaggaaat attactgatg ccacagcaga aagttttatt
    40081 caggcattta aagaacattt gatttgcaat cattatctgg cacaggaatg tatagccgga
    40141 atgaaggaga tgggttatgg aagaattatt aatgtcatct ccacctcagt gaaacaaccc
    40201 attaagggac ttggtgtttc aaatacgatt agagcagcag taggcaactg gagtaaaacc
    40261 atgtcaatag aactggcgcc atttggaata acggtaaaca atgtattgcc cggtgcaaca
    40321 ctgacatcac gattggaaca gattattgca gataaagtac aaaagacagg aaagacagaa
    40381 aaagaagttg tagaatcact gactacagaa attcccgcca aacgatttgc caatccggaa
    40441 gaaacagcgg cagcaattgc atttttagct tctccggctg cgggttatat caacggcacc
    40501 aatgttgttg ttgatggtgg ccgtacagca tgtctgtaaa attattgata aagcagaaac
    40561 ttaattttat ttaatatctg atcgttgttt aatggtcttg ataaagtatc ggtagcaccg
    40621 gcaaggagac atttttcttt ttcctgagca tcagagcctg aaagaacagc cagtacagga
    40681 atattcctaa attgagcggc tttacgtatt tcagtaatag catcaactcc attcattacc
    40741 ggcatcaggt catccattaa tacaatgtca acatgcggat ttttttcaag tttgtttata
    40801 gcttcttttc catcataagc ataaataaga ttggctcctg cactttcaat taatgtagtt
    40861 aaggcataaa tatttcgcaa atcatcatct gccatcagaa tggttttccc acttaatgac
    40921 gactcatgaa aagcctgctc aacaacggtt tcttcctgtg gaatattttc aggtgcttgt
    40981 agctgattca ttacattttc gagttgcttt ttgtttaaag gtttgtgatg aaaagccgtt
    41041 atattaaact catttattaa accttgagta ggtttaagct cgctgatgat atggatagga
    41101 atattacaaa gcttttgctc tgaacgtata tttttaagta aggttccgcc atcaatgtca
    41161 ggtagattgt gatcaaggag aatgaagtgt ggatgataac tcttagcaag taaaatacct
    41221 gtttcgccat tctgtgatgc aatagctttg tagttaaaaa tgtgacagta atctatcagt
    41281 gtcttaagaa atgtaatgtc tttttcaaga attagaatta ccttatcgaa gggtgtgata
    41341 aaatttctgt catcggtaaa gtcattatca ggaaactttg aaacaatggt attgattaat
    41401 ggtaattctt tttttgtatt ttcagtttct tgtttctcag tttctgcttg tgtgattact
    41461 ttttttacag gattatcatg tttgctttcg catgatgcaa aaaatggaat aaaaacagta
    41521 aacgtacttc ctttaccagg cgtactctct aaaacaattt caccaccgag aatttgaact
    41581 atttcgcgac tgatggtaag accaagtcct gtaccaccat attttctgct tgtactgtta
    41641 tcggcctgct taaatgcttc aaatacctgc ttttgttttt caacgggaat tccaattcct
    41701 gagtcagtta cgctaattgc aataatatct tcttcaaaga gtagattgtg agtcttgcaa
    41761 tcttctgtag tcgcttttct gaaatcaaga ctaacatgcc cgtttttatc agtaaacttc
    41821 aaagcattgg agattagatt ttttataact tgttccagac gcaacttatc gctcaccatt
    41881 tccggataac aattatccca aacttttacc tgaaagttaa tctgtttttc gctggcaaat
    41941 tctttaaaca acaggttgat gtcgtttgca acggtctcaa tattcatttt ttcaaaaaca
    42001 aattccagct taccgctttc aatttttgaa atgtcaagaa tatcattaat aagcaataat
    42061 aaatcagatc ccgacttatg tatgacactt gcatgttcaa tttgtttgtc cgtgaggttt
    42121 ttatttttat ttcctgcaag cagtttggca agaatcaaaa tgctgtttaa tggtgtgcga
    42181 agctcatgcg acatgtttgc aagaaatgcc gatttaaact tattggtttg ctcaagctgg
    42241 gttgctttaa aagccagtga ttgacgtgct tcttcaacag caagacttct ttcttcgagc
    42301 agatgtgctt tgatttcaag atttatatta gcagccttta attcttcttg ctgctttttc
    42361 aattcttttt gtgaaatctc caactcctca gatttaatag taaggttttg atttacctgt
    42421 gccaactctt gtttttgtgc taccaactcc tcagattgca taatactttc ttcaagcaat
    42481 tcgcgagtga gtgcattgga atatactgca taaatacttg cagataccgg ctcaattagc
    42541 cgttgcagat aatttaatcc tgaagcagtg agtttgttta atgatgcaat ttctaaagca
    42601 ccaattacac gaccggatgc aaatagcggc atgacaaaaa cagaacaagg catagactta
    42661 ttttctccgt cttttctaat gtcaaaaagc aaacttgttt ctattctttc gttattgtca
    42721 atacattttt ttaccagctg atgtttaatc cactctatgt gtaagtcggt aagttgatga
    42781 cttctacagt agattccttt taagtcaact tcatgcagcg attcatcttt tgtgtctaat
    42841 aaaaacaccg ttccatgaca ggcactggaa tattcaacaa cttcttttat caggttattg
    42901 caaagtacct gcatatcgag ggtataatct ttattcaaga cactgattcg ttcaagacct
    42961 tctgaaatcc atttttgcga agcatcttcc tcgctggccg cctttaattt gctgcgcata
    43021 tcaagaagcg ctttaccctg aacatcattt tcactcaatg gttcaaagtg ataatcaaat
    43081 tttccctgac ctatattgtt ggcaaactct gctgtccgat tcaatccgct tacgtgcatt
    43141 ttaagtgcat ctatcatttc gccaacggca ttcttaggtg ttttaatatt aagttcaggc
    43201 aactcacctt ttcctaactg caacagatag cgtcttattt tcattaatgg gttaacaacc
    43261 tgtttattca gaaacaaagt aattacgata atgaaattaa taataaccat tgccaaacct
    43321 aaaacaacag ccatcaaaac tataaacgac tgaatcattt ctttttgttt agcggtagtt
    43381 tcagcgcgtt tttttatgtt gaagtcgctt aacagagcag taatttcctg tacttgtggg
    43441 gtaatttgtg tttctaatat ctctacatta ttttctgtac taacacgagc tttctcaatc
    43501 agctgtgatt caaaataaat taaacggtcg tatttttcga atagaccgac taaggcaagc
    43561 tgttcctttt gttgccaaga ttctgaaagg tgtttaagtt ctttttttag tgcaggatat
    43621 tgtgtttcgt taataaactg aagttcgctg atggaggcag ctttattttt gcgaaaagct
    43681 acattggttg cctgaagcca tgaattgttt actaactgag taaggtcgga aattgccgat
    43741 tgcgaaggtg tagttacttc tgcaatttct atggtgatgt ttttgctttt gtcaataata
    43801 taggtgctta taaaggcatt ggctaaggtg ataacaataa gcaagataaa aatgacgtaa
    43861 agctttttat caattttaaa agaccattta ttggattgca ttgctccggg tataagaaat
    43921 attaaaaaat tatacgaggt ggcagtgtaa atggttacct gaataaaaca aaaaatcccg
    43981 ctatgggagg cgggattttg agtgaggtcg cgagcagatt cgaactgctg tacaaggttt
    44041 tgcagacctc tgcctagcca ctcggccacg cgaccctatt tactggcggc aaatgtaaaa
    44101 atattttgct tgcggcaaca agagtgaaaa tactgaaata aatggtttta aacgattaat
    44161 tagccgaact ggttacatta agcttcaatt acaaatgtag cttcactaat aaaacccttt
    44221 acaggaggat tatatttggt tatggccact tttattttac tgcatattgt aaaacgttct
    44281 ttcatttttt gtgcaatgcg attgccaaca tgttctataa gttttgacgg aatggccatt
    44341 tgtgttttaa ccatttcatt gacttcgaca taatcaatag tctgagtcag attatcggag
    44401 tttgctgcaa tactcaggtc ggcataaaaa gtgatatcca cacgatagcg gccaccaata
    44461 acggtttctt gtgataaaca gccatggtgc gcataacatt caataccgga taaaattagt
    44521 tggtgcataa tgcaaaattt tggatacaaa agtaattcat tggcaaatga ttctttttct
    44581 ttaatatcgc cttttattta tgaaatatag aaatgaaaat tcgtcttgtt ttattactca
    44641 cttttataaa ccttagtctg ctaatggcac agcaacccat aactcttaat gtgtctcagc
    44701 ctgatcaaga ctggcctaac agtgttactt atcatatttt tgtccgctcc tttagtgatg
    44761 gcaacggtga cggcattggc gaccttaaag gtattactgc caaatgggac tacctcagaa
    44821 aattaggtgc agaagcaatt ttaatcagcc cggtatttgc ttcgccttca taccataaat
    44881 acgatgtgac ggattataat aaattatcac cggaatatgg aaacatagaa tcgtttcaat
    44941 actttttaaa agatgctcat gcagaaggat ttaaggtgat tttagatata ccactaaatc
    45001 attgcagcag caaacatccc tggtttttag aagcagtaaa aaatccggca agtgcaatgc
    45061 gaaactattt tttgtggagt aacaaccctc ctgcaacgga gaaagacaaa tggcatctgc
    45121 caaaagatga aaaagggaat acagttggaa aagcatacta ttatggtttc ttcagtcctg
    45181 aaatgcctga ctggaactac gacaatacac aggttgtgga ggaagcacaa cgaattttaa
    45241 aattttggat tgatttggga gtagatggat tcagattgga tgctgcacag catattttta
    45301 atgatcacat caaaaatgtt ggcttctgga atatgatttc tgcttatgta agaagcatta
    45361 acaagcatat ctttttagta ggcgaggtgg acaattcttc tgatgttgta gcaccatatc
    45421 ttaaaccact cgatgcttgt tttgactttg atttgtcacg tgaggttgtt caatctctga
    45481 agaatgagaa tgcaggtaat tttcctgtga gaatggaaaa aataatcacg cactataaaa
    45541 cagcaaataa agattatagg gatgttattt tccttaccaa tcacgatcag aacagattga
    45601 tgagtgaggt tggaaataat gttgacaaag caaagcttgc agccaatatt ttatttaccc
    45661 ttcccggaat tccatacatt tattatgggg aagagattgg gatgaaggga gaaaagcctg
    45721 atgagtttat tcgtgaaccc atgttgtttg ctcctgaaaa agaagatgaa atgcgaccta
    45781 aatggatgaa gccgaaatac agtacagata aaaccgttga gcctgcaatg gttcagataa
    45841 aagaagatca gtcgctgtgg aatcattatt caagaatcat ttctttgcgc aaagacaaca
    45901 gagcgttgca gtatggtcaa tttgaaaact cttctttgag caataaaagt ataatggcat
    45961 attaccgcat ttataatcag atgcgtgttt tgataattaa caacgttagt gcaacggccg
    46021 ttacacttac taaagaagaa aaagacaacg aacttgtaaa tccgattttt cagacatcaa
    46081 agcaaactac tgttaaagga aacaaaatta cggttgcgcc atacagtaca attatactca
    46141 ccaattgata cgtattaaaa attaatacca atcctgaaag aatgtaatat gcaggagcag
    46201 tgtaaaccgt agttctttca gggcttagaa aatatattgc tggtattaaa caaacttcaa
    46261 cgcagtttct aatcttttaa tcacttcgtc ttttccaagc agttgaatca ttggaaaaag
    46321 gtcaacacct tgtgcctgcc cactaacaaa tacgcgaaag agttgaagta cttcaccggg
    46381 tttgatattg tttttttcag cagtagtttt aaaagtttgc tcggcaagtt gactactcca
    46441 gtcctgtaaa gtcataaatt catcagagag cattttgaaa aatgaaataa attcagtttt
    46501 ccatttttta gagattactg tctgatcaaa ttctgacggt gctttaaaaa gatatattcc
    46561 tttttcaggc atttctttct caaactgaac acggggtttt aacaatgcca cacatgctgt
    46621 tagataatta tcagagaggc gcacatcatc tgcactgatt ccagtgcgtg ttgcaaccat
    46681 aggtttaatt cgttttgcaa tggcttcatt gggttgtcgc agcatccatt gtgtgttgta
    46741 ccattttgtt ttttcaagat cgaaacgtgc acctgctttg tgaacatgat ctaaagaaaa
    46801 tgctgcaatg agttcgttca tattaaaaat ttcctgcgct gttccgggat tccaaccaag
    46861 cattgcaagc atattaacaa atgcttcggg gtagtaacca ctctcgcggt agccactaga
    46921 aacctcacct gttgaagggt ctttccattc aagaggaaag acaggaaatc ctaatctgtc
    46981 accatcacgc ttgctgagtt ttcctttacc atcaggtttt agtaataatg gaaggtgggc
    47041 atagtgtggc atttgtgcag ttagtccgag ataacgataa attaatatat gagcaggagc
    47101 cgaaggcagc cactcttcac ctctgatggc atgtgttatt tccatcaggt agtcatcaac
    47161 aatatgtgct aaatgatagg taggcatgcc atcactttta agcaacactt tatcatcaac
    47221 taaattagag tcaaagctaa ctacaccacg aatgagatct gtaaaagtta tagtttcatt
    47281 ttccggaact ttcaaccgga tagcataagc aacattcttt gaaagtagtt ctttaacttc
    47341 gtcaacactt aatgccaatg aattattaag cttcattcta gttgcactgc cataagaaaa
    47401 ggtttctttt ttttcttctg catttttgcg ctgctgttct aattgatgtg cagtatcaaa
    47461 tgcataatag gcattgccgg actcaacgag ttgcatggca tagcttctgt aaatttcttt
    47521 tcgttcactc tgtctgtatg gcgtatgtgt tccaccaaca gaaacacctt catcaatggt
    47581 aatgccacac cagtgtagtg catcaatgat atattgttca gcaccgggaa caaagcggtt
    47641 ctgatcggta tcttctatac gtaataaaaa atcgcctgca ttttttttag caaacagcca
    47701 ggcatataat gctgtgcgca caccgcccat gtgtaaaggg cctgttgggc tgggagcaaa
    47761 tcgtgttctt attcttcgct gcataaaata aattctgcgg caaagataat cgagtaaaag
    47821 attgagaagt ttatttctta agtgaagtta tgcttgaagt gagtttttct tcaatttttt
    47881 tgtaatcacg taactgatca aaatattcag gctttgtttc caactctttt atcttgttgg
    47941 tgacaaattt tttgagttta ctcagtgtat tggacgtttg catattcata aactttgagc
    48001 tgctgttttt tgcagcatca gtcaaagcgt caacaccttt ttctgttaca gcaggcgaac
    48061 tatgttcgag gaaggttccg agattttgaa taattcccat ctttccaaat cccgaagcca
    48121 catttaatgc atttatcatg taatcataat tttcatcgct gccatactgt gcataaagcc
    48181 ccgatagtgt gtaaagaagg ttgttgttat tatctgactc atattttttt agttcggaca
    48241 attgcttggg attttttgtg atgtaatgct ccaatgctgt agtcattaca ttgtatgatg
    48301 aatcatttat agcaatatta tctaaagtag aatcatcaaa atatttatat aaagttgaca
    48361 aggcagattc cctaacatgt gctttagcat cttgagttac catttgttgt aacttatttt
    48421 ttactgcgct atcggctttt gctgctgcac ctatatttct caaagcaagt gctctgatag
    48481 aagggctttt atcgtctaat gcactgataa taatttttgc ttctgcacta ttgccttcat
    48541 agccttttgc caaagcatca attgcttcgt aacgatcaag aaacaatggt gcatgcttgt
    48601 actgaaacat ccattcatca ttggtgtgat tgtctttttt tgagcacagc aattgttttt
    48661 ctgcatcaac attaatcaac tgaggtttgt tgttgcaatc aaaaaacaaa tattcttttt
    48721 gttgcgtaag tgtaacctgt tcgcgtctca cttttccgtt ctcataaata tctattgaaa
    48781 gaggaagttt atagagtggt gtttcctgaa ggttttgttt ttgctcgatg atgacagttt
    48841 gttttttcag tgtatcgttc cactgataac tgatgtcaat aatgggatga cctttagcaa
    48901 aaaaccactg attgaaaaac cagttgaggt cttgtccggt aacagcttca aaagccaaac
    48961 gtagattatg tgcttcaaca gggctgaatt tattgtcgtt taaatatttt ttcaatgaca
    49021 taaaaaaggc atcatcgcca acatatttac gaagcatgtg taacacacgt ccacctttag
    49081 aatagctgtg tctgtcgaac atatcttcgc gcgaattgta atgaaagcga ataagatcaa
    49141 cctgcttgta tttggcttca gcaagatatt gattcagatc atccatacca cctttgtcag
    49201 cttcttcacg accgtatttg tattcatccc acagatattc accataggta gcaaatgact
    49261 cgtttaatgg tatattactc cacgattcac aagtaacata atcgccaaac cagtgatgaa
    49321 aaatttcatg cgctatggtg ctttcattgt tgccatctag gagttcgcgt ttggtgcggt
    49381 gtaaatactc accataaaga atagcagaag tgttttccat agcaccgcta acgtaatcgt
    49441 gaacaacaac atttgatagt ttttcccaag gatagtcaac gcctaatttg cgagagtaga
    49501 agtcgagtat ttcgggtacg tttccaaaaa tatcttttgc atatttttca tattcagggt
    49561 caacataata gtttacttca atattgcgcc aacggtcttt aactacttta aaattgctca
    49621 ctgccatcat agtcagatag ggtgcagcag gtaatgattg tttccatgta tctgtacgtg
    49681 tgccgtctgt atttttaacg gaggatgtca tcaaaccatt gcttagtgtt ataaatgagc
    49741 tgtcaacagt tatgctgatt tcctgagtca tattttggtt tggagaatca attgtcggga
    49801 accaaacgga gttactttgt gtttcaccct gcgtccaaac ttgtcgtggt ttgttagggt
    49861 ttttaccatc ggcattaatg aagtaaagac ctttatcact aataattgct gcactgcctc
    49921 caatgttttt taattcgtca ggctttgata tgtagtcaa
//
LOCUS       DAOQNP010000012        49778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_203_length_49778_cov_8.302938, whole genome shotgun sequence.
ACCESSION   DAOQNP010000012 DAOQNP010000000
VERSION     DAOQNP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 49778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49778
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_49778_cov_8.302938"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /gene="folP"
                     /locus_tag="PKY09_03025"
     CDS             <1..686
                     /gene="folP"
                     /locus_tag="PKY09_03025"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB11857.1"
                     /translation="EGATFIDIGGSSSRQGSKMSSVEEEWNRIGKAITLIKLHFPEVL
                     ISVDTVYSVIAKRAVDSGACMINDVSAGLIDSRMIETVAALKVPYLMMHMKGLPENMQ
                     DNPVYDDVLKEIIDFFSKQLSLCRKVGITDILIDPGFGFGKTIAHNFELLAKLNLLKI
                     VGLPVVAGLSRKGMIWKTLDVSPAEALNGTTALNMVALMNGTRILRVHDVKEAMQCIK
                     LFNKLDAAN"
     gene            complement(725..1384)
                     /locus_tag="PKY09_03030"
     CDS             complement(725..1384)
                     /locus_tag="PKY09_03030"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNB11858.1"
                     /translation="MTIHREGKNWVWGTFITVLLINFLVLNFRAEHDWITVLVLIVTL
                     VFFLLILQFFRYPKRNITRNENYVIAPADGKVVVIERTEESEYYKDKRIQVSIFMSPI
                     NVHANWYPMSGKIKFLRYHKGKYLVAWHPKASTENERSTIVVEKDSNKTILLRQIAGA
                     LAKRIVYYPRENDLVKQGAEMGFIKFGSRVDIYLPLTAKINVELNQKTKGGVTVIAEL
                     V"
     gene            complement(1411..2688)
                     /locus_tag="PKY09_03035"
     CDS             complement(1411..2688)
                     /locus_tag="PKY09_03035"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010901058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNB11859.1"
                     /translation="MADSKKAKQNHEEENPFEEMIKRLDVAAKIMKLDSEVYEIMKKP
                     SKLVYCSMPVKMDNGKTKVFEGFRVVHSTALGPSKGGVRYSTYVNENEVMALAAWMTF
                     KCAVADIPYGGAKGGITCDPSTMSKGELERLTRAYTNAMVDVFGVDKDIPAPDMNTGP
                     QEMAWIVDEYSKLKGGFTPGVVTGKPIHLGGSLGRSEATGRGVMTATMEAMAKMGLNP
                     AKCRAAVQGFGNVGSITAKHYEAKGLKIVAISDHTAAFYNPDGIDIDKAIKYRNSNKG
                     VIKGFKGGKLISNEELLTLNIDVLAPCAMENQITDENAAKIKAKLIVEGANGPTTDEA
                     DHILSKKGTVVIPDILANGGGVTVSYFEWGQNRSGLYMTEDEVNTKADHWMKQAFHNV
                     WNTSVKHKTTMRIAAYIYALGKIELGIKSRGHY"
     gene            complement(2922..3941)
                     /gene="murB"
                     /locus_tag="PKY09_03040"
     CDS             complement(2922..3941)
                     /gene="murB"
                     /locus_tag="PKY09_03040"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNB11860.1"
                     /translation="MPMRENVSLKNLNSFGIEAKARYFIEINSEDELKSFLLQHKKTA
                     LPLLVMGGGSNMLFFKDFDGIVLKNNLNGISVVEENETYVKVRVGAGEVWHNVVMWSV
                     EKNLGGLENLSLIPGTAGAAPIQNIGAYGVEIKNSLVAVEAMAIDSAEKKVFSNQECE
                     FGYRNSVFKNAVKGKYIITAIILELKKQPQFKTEYGAIQSELEKLGITTLSVKAISDA
                     VIAIRKSKLPDPAMIGNAGSFFKNPEITDKEFEKIKQQHPNIPHYPAGENKIKLAAGW
                     LIEQCGWKGFREGDAGCHKNQALVLVNYGNATGAEIYNLALKIKESVYNHFGVHIEPE
                     VNIIQ"
     gene            complement(3941..7582)
                     /locus_tag="PKY09_03045"
     CDS             complement(3941..7582)
                     /locus_tag="PKY09_03045"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11861.1"
                     /translation="MRRRIQFNLTFLSFIICFFICTSQKVQAQFWLPVGSGVNNNVYA
                     MTKDTVNNILYIGGRFTDASGVTAIRVASWNGTSYTAIGDGFDSNVFALYYDHSNNTL
                     YAAGSFLSSGINPITRIAKWDGTQWQPLGTGCNDEIYALTGDANGNIYAGGIFTTAGG
                     ISTERIAKWNGTTWNALGSGIGSGSNYVEALTFYNGDLIAGGQFGIAGGVAVSGIARW
                     NGTNWFDLNGGVSGLSMRVTAFKEVNGELICGGTFSSAGGVSSNSVAKWNGAAWLAIP
                     GGISGGQAKVKSLGYLFNTLYVGGNFSLAGGNAVSNIAAYDGTTWSDLSGGTNNQVDA
                     LLNYGNEMHVGGAFTMAGGNPATYIAKYRTTCLTNASISTTQTSCFGQCNGTATVTAT
                     GNAPFTYQWSTTPVQTDSIAADLCAGNYTVTITDNIGCSITQPVTITEPAQFTVSFSS
                     NNPTCFGLCDGNALASNNGQGTVTYNWNTIPPQNTQTAIGLCEGIYSVVLIDSAGCMV
                     TDSVIITNPVPNTLSLTLSNPTCYNNCDGIAAVVSTGNAPFQYLWNTIPAQQTDTATG
                     LCAGVYYITVTDSTGCMATDSIEIINPTAATLQFSTAATTCFGSCNGSASVVSSGTSP
                     FTYLWNTTPVQTTDTATNLCAGTYFVTVTDSNLCAVTDSVVITEPVANLISFASQPPA
                     CVTSCNGLLTATSTGTIPFIFQWSNGDSTSTIDSLCTGVYTITISDSIGCTVTDSVSL
                     NPAPPLPVTFTTQFAGCNGVCTGAVSAVITGINPTSYIWSTGDTVASIDSLCQGLYTV
                     SITDSLGCIVTDSVQIISQPVLLNPVATAPTCAGQCNGLASINPSGVAPFQWQWSTGD
                     TTSLSIDSLCAGVYYITITDSVGCIGADSVTIIDPPAITYSASHTDATCANLCNGIST
                     ITATGNGTLSYLWNTVPPQTDSTNTNLCFGYTAYTITDTNNCSVTDSVLIFEPSPILI
                     GNNFLGITCNGNCDGFVRAIPSGGTPGYTYLWSNGATFDSIFNTCAGTYTVTVTDANL
                     CAAVDSFTFVEPDTIVISFTVADASCPGCTDGSIVATATGGTPPYDYLYPTLGIADSA
                     ATNLGMGYYLFCAQDFNNCMQCDSVFVDEATAINSLSAEIKEIKIFPNPFTEKAFLSI
                     TTTESKDFKLYFFDVAGRMVNIHYSNIGNNGKKQTFSIENQGMIPGMYYVRIMSANEV
                     VAIGKFIIN"
     gene            complement(7616..8425)
                     /locus_tag="PKY09_03050"
     CDS             complement(7616..8425)
                     /locus_tag="PKY09_03050"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNB11862.1"
                     /translation="MLEKIKATAEFISKTTGFSPETGIILGSGLGGLVKEIDIVKELN
                     YSDIPGFPVSTVKGHGNKLIFGTLNGVKVVAMQGRFHFYEGYSMQEVAMPVRVMKFLG
                     IKNLILSNASGGVNPKYKVGDLMILDDHINLMGTNPLIGPNNDDLGPRFPDMGEPYDK
                     TMIKNAKAIADKHGFTCHTGVYAAVTGPTFETRAEYKYIKFIGADCVGMSTVPENIVA
                     RHMNLPVFAISIITDLGGSDEPEFITHEEVLKVANAAEQKMTIIIKELIAQ"
     gene            complement(8397..9449)
                     /gene="lpxK"
                     /locus_tag="PKY09_03055"
     CDS             complement(8397..9449)
                     /gene="lpxK"
                     /locus_tag="PKY09_03055"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNB11863.1"
                     /translation="MKSLRILLLPFSWLYGMVVLLRNLLFDVGLLKSRTVDIPVICIG
                     NLNTGGTGKTPHTEYLLSALSNYKTAVVSRGYGRNTSGIVEVHVDSQPNDTGDEPLQI
                     KRKFPIVPFIVSEKRSEGIDYLLKHYPNTEVILLDDAFQHRSVKAGMNILLTQYNDLF
                     INDCLLPAGNLRESASSASRADVIIVTKCPTAITVEQKKSIISQLPSKPVFFSSFSYD
                     NLQSLNSDEKLNLRDLTEYEILLITGIASNNSLLGFLSTHSKKIKNISFKDHHNYCAK
                     DLENIIAIFNNLASEKKIIVVTEKDAVKFNSSELKPLTKNLPVYSIPVTVVINENEIK
                     FLDTVKAYVGKNQSNS"
     gene            complement(9539..9802)
                     /gene="rpmA"
                     /locus_tag="PKY09_03060"
     CDS             complement(9539..9802)
                     /gene="rpmA"
                     /locus_tag="PKY09_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNB11864.1"
                     /translation="MAHKKGEGKVKNNRESHSKRLGIKIYGGQHAIAGNIIVRQRGTK
                     HNAGEGVGMGKDHTLFALVDGKVVFKKKTDNKSYVYVTPVAQA"
     gene            complement(9823..10134)
                     /gene="rplU"
                     /locus_tag="PKY09_03065"
     CDS             complement(9823..10134)
                     /gene="rplU"
                     /locus_tag="PKY09_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNB11865.1"
                     /translation="MYAIVNILGQQFKVEEKQQLFVHRLEGAEGEEVKFNDVLLVDNG
                     GSIKVGKPSLSNASVSAKIISHVKGDKVLVFKKKRRKGYKKMNGHRQYMSKIEINSIN
                     A"
     gene            complement(10307..12688)
                     /locus_tag="PKY09_03070"
     CDS             complement(10307..12688)
                     /locus_tag="PKY09_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HNB11866.1"
                     /translation="MKKFFTLLLAASFASTVNAQNFNISLLGTLPYPNDDLANIGGWV
                     SPSGEEYALVGSESGLSIVDVSNPANPTEVILVPGPQSQWREVKTYDHYAYVTTEGGG
                     GLQIVDLSQLPNAAPAIDWTGNGAISGQLDNIHSLHIEDGYVYLHGSNLFQGATIIAD
                     IATNPLSPNYVGHTPGAYVHDGYARGNYYFGCHIYNGDFTIYDVTNKANPIPLGIKVT
                     PGQFTHNAWLSTNSQYLFTTDEVSDSYLTAYDISNPANIIEMDRIQITPGSGSIVHNT
                     HIIQKNGADYAVTSWYKDGVVITDVTRPQNMVNVGWYDTYTQGAGDGFSGDWGVYPYL
                     PSGNLVVSDINNGLFVLGPTYQRACYLEGVVTDSVTGNPIFNATVKVLSTNITKNTKA
                     NGEYKTGTATAGSYNVEVSAAGYFTKTINGVSLTNGNVTQLDVQLSPLASTSVSGQVV
                     EAGTGNPIPNATVTIQDSALVYNTTADASGNFTIPSMVVGNYQVVAGSWGHRTTCFNS
                     NIMLNPAPLVITLNKGYYDDFSFDFGWTITGTTQNLWERGVPHGTPAVFGPGFANPDA
                     DDQTDCLNQAYVTDNNPNASSSGSNDVDDGYTLLTSPVFDPTYLTNPTINYSRWFYNA
                     GGNSNPNDSLIIRISNGTTNVVLETVRASTPGMGTWVSQSFNLNGLIPVTNTMQISVY
                     TADQAMSGHVVEGGFDKFEVNGTVGISTLSATANTLSSSPNPFKNESRLHIGENGGNL
                     TITDITGKMVETLKVQKNEQIFIGKSFKKGVYLVKLVSDKGTVSSIKLVKTGE"
     gene            complement(12942..15254)
                     /locus_tag="PKY09_03075"
     CDS             complement(12942..15254)
                     /locus_tag="PKY09_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNB11867.1"
                     /translation="MKKIYLLLIGVFVLNISQACDTTSIAKTQWTVSYVDSEELVGEG
                     PNNGHATHCIDNDTATFWHTKWQGITTPYPHEIQINLGAVYPVNGFSFLTRDNNTGGR
                     VKNFHVYLSNDSITWGSPQAAGVLIYPNPNSGAQQTAAIYFGAVNAQYIRLVFDSSYS
                     GIHVMCAELNVFQDLSCGATGQNNQIISLNPVPKQSTVSTPITLTGTTTSGLPISYSI
                     VSGPATINGDTLTLTGATGTVMVQADQAGNALYYPASAQLSFDVIDLTTYFPAVSSKL
                     TGDYDLEMPQLYPYLLHTNASIAEPTLLSIASVEYNINGVVLNTIYNDGDYQVWWTPP
                     AYGNYTIYVTATGNNGNTDVDTINLNVTNTFATQNAQTITNGVIDMGTIGSQWFYGTY
                     TLPQSVGVYDSIVANLHISCPAVPGGCDDWDRLAWVEFKAPNGEWMELFRYITPYRVA
                     CNHQIDVTDYASVLQGEIELRMYIETWGTGGWQINLDFDYYASPPAFLYSTIEQLWKG
                     NYNFGNPLNLQPMDTVVIKPYSNVSKATFRLVTTGHGWGSNNTGNAAEFYHAIHDLKI
                     NNVTTFTQDLWTDCNPNPDGCTGQQGTWQYNRAGWCPGTIPKPYFYDLTPYLSQAPFN
                     FEYIFQTSYQDICHPNNPACVSGVTCANCNDGYNPSYRIGGYLIRYSNLPLVLGVQSH
                     TPESELNLNFDIYPNPASGFFYLVPENKFGNTVCSIHNISGETLRTYYFNTSEQLAAY
                     SFNLSGLSKGVYFVRLQSEKNIMTKKIMFQ"
     gene            complement(15329..16489)
                     /locus_tag="PKY09_03080"
     CDS             complement(15329..16489)
                     /locus_tag="PKY09_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB11868.1"
                     /translation="MRQQTLLNHITGIIAGFDFSVPLHHYLKNHFRQNKQLGSRDRKI
                     ISSGCYDYYRYALLLPNRSVEEQLALSFSITNEKNLLSDYLSEKYQIAKNESNSLSER
                     LEFIRKQDGITPDVAFSFLDLLSKEIDKEAFAASMFQQPLVWIRIRKGFEKSVLDELK
                     LKEIISFQSTENALGFAPAIPLTALESYLKGHFEIQDLSSQQTLSLLPDNCGQRMWDC
                     CSGSGGKTLLLADRFQKAAFLLTDLRTSSLQNAEERLKKAAVRNYKTMQADLTKDLNF
                     NEKFDVIIADVPCSGSGTWSRTPENFWNFDKNKIKDYANLQFTITKNATKHLKNNGLF
                     LYLTCSAFGMENEENTKRIAKHCNLNIVEQTFFHGYKHQSDTMFSCLMKLKS"
     gene            complement(16640..18646)
                     /locus_tag="PKY09_03085"
                     /pseudo
     CDS             complement(16640..18646)
                     /locus_tag="PKY09_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773400.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
     gene            complement(18698..20560)
                     /locus_tag="PKY09_03090"
     CDS             complement(18698..20560)
                     /locus_tag="PKY09_03090"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNB11869.1"
                     /translation="MSKKVAHNYTEDSIQSLDWKEHIRLRPGMYIGKLGDGSSRDDGI
                     YVLLKEVIDNSIDEYVMGYGKQIDVTVENGSVTVRDYGRGIPLGKVVDCVSKINTGGK
                     YDSQAFQKSVGLNGVGTKAVNALSTYFKVQSVREGKTTIAEFERGELKKQQKEKSCDL
                     ADGTLISFIPDNEVFIDYKFNSEFIERMLNNYAFLNVGLRLNFNGKEFFSQNGLLDLL
                     KANLSEEVLYPAVHLKDNDIEIAFTHTPSYGEEYFSFVNGQHTTQGGTHLAAFREAVV
                     KTVREFYKKEFDPSDVREGIEAAVSIRIQEPVFESQTKTKLGSQNTYPNGPSLRNWIN
                     DFVKTNLDNYLHKHPETAEIWLKKIQQSERERKEIAGIKKLANERAKKANLHNKKLRD
                     CRTHLNSKSPERFESTLFITEGDSASGSITISRDVNTQAVFSLKGKPLNCFGLTKKIV
                     YENEEFNLMQNALNIEDGLDELRYNRIVIATDADVDGMHIRLLLLTFFLQFFPELVKN
                     NHVYILQTPLFRVRNKKETIYCYSEQERVAAIEKLGSKPEITRFKGLGEISPDEFKNF
                     IGRKMRLEPVRIEGEGSVQHLLEYYMGKNTPERQEFIIGNLKLEEELEPEIAEA"
     gene            20993..22549
                     /locus_tag="PKY09_03095"
     CDS             20993..22549
                     /locus_tag="PKY09_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNB11870.1"
                     /translation="MEKQRILWADDEIDLLKPHILFLNDKGYETVTANSGNDALEIFR
                     KEPFDIVFLDENMPGLSGIETMMRIKSINSNIPVVLITKSEEESIMEDAIGSQIADYL
                     IKPVNPNQILLSLKKNLDNKRLVSEKTNTQYQQEFRQIGMMLGDRLGHAEWAELYKKL
                     VYWELQLDKVRDESMFEILIQQKEEANRLFSKFVESNYLNWIRNPENNAPLMSQHVLK
                     RKVIPVAETSDVPVFFILIDNLRFDQWKVIEPIISELFRVTEEDTYFSILPTATQYAR
                     NSLFAGLMPADIEKMHPKWWVNDDDEGGKNLHEEDLLKDQISRLRKNIKSSYTKITNL
                     DAGKELVDNIPNLFGNKLNVIVYNFVDMLSHARTDMQVIRELADDEPAYRSITLSWIE
                     HSPLLEALKRIAQKNVKLIITTDHGTIRVKEPSKIIGDRNTNTNLRYKQGKNLNYEKR
                     DVFEIKNPADALLPRLNVSTAFVFAKQDKFFAYPNNFNYYVNHYRNTFQHGGISLEEM
                     IVPFVSLVSK"
     gene            22555..22974
                     /gene="tsaE"
                     /locus_tag="PKY09_03100"
     CDS             22555..22974
                     /gene="tsaE"
                     /locus_tag="PKY09_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNB11871.1"
                     /translation="MSEHVFFCNSPDELNSVAKQILAVIGESKIVLFKGEMGAGKTTL
                     IKALCKQLNVADNVSSPTFSIVNEYHAANEVIYHFDFYRINNLREVYDMGYEEYFFGG
                     HRCFIEWPEKIEGIINFDYTLVQVDAKDEIRTIIVTI"
     gene            22971..24197
                     /locus_tag="PKY09_03105"
     CDS             22971..24197
                     /locus_tag="PKY09_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNB11872.1"
                     /translation="MTTASKSGGMFSSESVLLPRESMLEVQKTKREVLIGIPKEISFQ
                     EHRIALTPDSVAVLTSNGHQIMVETGAGDSANFTDTDYSEAGARIVYSAKEVYTADII
                     IKIAAPTNDELDLMHDKQTLFSVLQVNMQSKDFFLKMAKKKITAIGYEYMKDDSSLMP
                     VIQAMSEIVGRTSILIASELLSNTTGGKGELLGGIAGVPPSEVVILGAGTVGENAART
                     AMGLGADVKIFDDNMYKLRRVIRNLGEHVFTSTLTQSTMLKALRTCDVAIGALHARTG
                     RTPIVVTEEMVSEMKFGSVIIDVSIDQGGCFETSKPTNHTQPTFREYGVVHYCVPNIA
                     SRVAKTASHALSNIFTPILLNVGEHGGLEGLLWKDASFRSGVYLFRGTVTNKYVSDLY
                     RLPFRDLNLLMASFTF"
     gene            complement(24256..25107)
                     /locus_tag="PKY09_03110"
     CDS             complement(24256..25107)
                     /locus_tag="PKY09_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11873.1"
                     /translation="MNKSFIYGIVALAIVISISSCKKDKSDADYDYSMATDMSLAENT
                     MEEVTKMADQAGDYGQLMTYRNSAPATVTVIGCAAVTIENVTATIKKMTLDFGATDVT
                     CIDGKRRRGKILVQYEGNYRDANHEHTISFENYFVNDYKTEGTRKITNTGKNNQNQSV
                     FAITADISISNPNGKTMTWQSQRTRTWIAGENTTFNTDSLNGVKDDIYAVTGSGSGKS
                     FNGTSFSANITEPLTVSLQCRFITKGKVELTPGEQAIRYIDYGDGTCDAIAAITINGV
                     TYYREIP"
     gene            25341..28400
                     /locus_tag="PKY09_03115"
     CDS             25341..28400
                     /locus_tag="PKY09_03115"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB11874.1"
                     /translation="MVRMVRRSIYLMLLFFPLMSLAQQTGIYTDVEWRYKSALDLLRK
                     EKFSAAREEFTKALEEPISEAARQDAMYYIGLCAAELYHPDAEFLLTDFISKYPQSIN
                     VSLAQFQLGRIYYKQRKYRNAITHFEAVDVAYLKNDEVIEYYFKKGYSYFTRKDYDKA
                     GKSLSQIINVESKYQTAAQYYNAHVEYSRNNNKAALEQFMKLKNSESFGPLVPLYITQ
                     IYFDQQKYDEALDYAIPVLKVENTQNKTEIIRIVAESFYRKGNYAKAISYFEDYSKNT
                     PQTTRQEKYQVGFCYYMLNDYAKAIDYLKQVLNPKDKLAQNAYFHLADCFLKTDDKQG
                     ALTAFESASKMSYDPQIQEEAMFNYAKLSVDLNMQSSTIRVMNDFMTKYPKSEHKDEI
                     TELLATIYMQTRNYKEALAALDKIKNRTATGNAAYQKVAYYRAVELFNDRDYEQSIRL
                     LNKAITTNIDPLIQAQAIYWKSEALYNQGKYDDAIKEYRIFIFTPAALKLSFYNRSNY
                     DMGYCYYKKGNYKEAATWFRKYVASRGETDNNRYNDALVRIGDAYFMQRDFANAENFY
                     SQAVGSKASSSDYSLFQRGIILGINGNNRAKADAMQQLITTYPKSNYADDAYFERAKA
                     LMVLDQDDEAELAYNKLISSYPTSPYIKKSLLNKGLIYFNNKNDEKAIETFKQVVTKY
                     PSTPESVEALNQIKNIYVAQGNPNAYFDYVKKIPNNAVSSGAQDSITYEAAEQRYMKG
                     DYKNASNDFAQYMSRFPNGSYILNATFYKAECEYMAKDYTNALKGYEIVNNATKNLFT
                     ERSLIRSSAIYYTQKNYDQALTAYQRLESNADYADNLLIAQTGSMRSYDKLKNCSQAI
                     VYADKLLSNDKTDPTVISEARLIKARCSMEAKDYAAAAKEFNLIAKGSNSEVVAEAKY
                     TLAFIQYKWGNYKESQKKCFEVIKQVPSYDYWIGKAFILLGDNYVALKDNFQAKQTYQ
                     SIIDNYEKNPSDPEDLKQIATEKLNVLITTEGVPQPQQPKEEENNEN"
     gene            28390..30096
                     /locus_tag="PKY09_03120"
     CDS             28390..30096
                     /locus_tag="PKY09_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB11875.1"
                     /translation="MKTNSTIESGMNTAIMLKKTIFFGALGLAFLQSKAQTRDTIKNI
                     GDENIIVVKDYQPTLSDAVKISDMPGADTSSIKEVKFKYTIEGKRADLPFNTSPIKPV
                     KIKDDEIKLLHKGYVRGGYGTHNTPYAELFFNALRSKEFDAGIHLKHHSSTGKIKNYG
                     KTDNSENALELFGKKFTDYGTLRTKLNFDRDVYRFFGYDGKSTVYSKAETRQLFSGIA
                     GEVGFDNFSKQESTYKFDAGLSFYGYSGKRDTSKTTENSVILAGKLQRKIADRDAFAD
                     IKIDYSRTKLPFDTLVKNTIVGVYPRYTLEFPEFRLIGGVNTEWEANSAAKFHLYPHV
                     EARYKLASDVLMIHAQVSGAMQKNTYKAMTAENPFTGDFVMPGNTNKKFDVSGGLNIK
                     LDKEILFSAGASFKRLKDAVYFENDSTGALNYTTFSTVYSDADVITLNGELVFERNEK
                     FNAHIGATYTNNKPDGLAKAWFVPAVEINLGGYVLLREKILFKTDMFYRGERFAAAYN
                     GDSFQRLKPYFDANLSVEYRYSKMVSVFLNINNIGVVQYFKWYNYPSYRLQLLAGASL
                     SF"
     gene            30324..30860
                     /locus_tag="PKY09_03125"
     CDS             30324..30860
                     /locus_tag="PKY09_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11876.1"
                     /translation="MAQRSRNTNRTHGLDVTISRLVPNFSKVPDQNFLFHESLTYSIV
                     AGQRIRALAGINFLAPAKGGVTRYGFHIGFLAGSKNPTSGLIYGPVLFHIVNKTAYDN
                     GALRNYHNTGLALAVGMYYVPVSQFVLSTKIAPAIYHYSVRKKNDIKVIDNNSDSRSE
                     TFYMGFYNLVSLSVGYRF"
     gene            30950..32791
                     /locus_tag="PKY09_03130"
     CDS             30950..32791
                     /locus_tag="PKY09_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNB11877.1"
                     /translation="MSKTNEVLDQVIIKFAGDSGDGMQLTGTQFTNTAALFGNDLSTF
                     PDFPAEIRAPQGTVAGVSGYQLHFGSVNIFTPGDKCDVLVAMNAAALKVNLKNLKDSG
                     TIIANSDGFDPKNLRLAKYPDGQNPIEDGSLGKYNLVVIDVTKMTRAALVDSGLGTKE
                     IDRAKNMFVLGFLYWMYNRSLDNSIKFLEEKFGKKQAILDANIKVLKAGYHYGDTTET
                     FRTRFTVQPAKMKPGVYRNIMGNQATAIGLIAAAQKGGIDLFFGTYPITPASDILHEL
                     SKYKNFGIKTFQAEDEIAAVCATIGASFGGSLGVTSSSGPGIALKGEAMGLATMLEIP
                     LVIINVQRGGPSTGLPTKTEQSDLMQALYGRNGEAPMPVVSSSSPADCFNTIYEACRI
                     AIEYMTPVIFLSDGYIANGAEPWQFPSADQLREFKVKYATETNGEHFLPYKRDEKLSR
                     PWAIPGTKGLEHRIGGLEKQHETGNISYDNDNHQFMVKMREAKVEKVADSIPLQQLDS
                     GPEKGKLLIVGWGSTYGAIKTACQMARSEGYDVAHAHLTYLKPFPKNFGEMLFKYDKV
                     LMPEMNMGQLSRVVRDKFMIPAISLNKVMGIPFSATEIKEKIVELMK"
     gene            32836..33876
                     /locus_tag="PKY09_03135"
     CDS             32836..33876
                     /locus_tag="PKY09_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNB11878.1"
                     /translation="MEATATTPTPLYTSKDLVTDQDIRWCPGCGDYSILKQVQTVIPE
                     LGLPREQIVFISGIGCSSRFPYYMETYGMHSIHGRATAIASGLKAARKDLSVWIVTGD
                     GDSMSIGGNHFIHLLRRNFNVNVLLFNNQIYGLTKGQYSPTSESGKVTKSTPFGSIDH
                     PFNPAALAMGADGTFVARSMDRDPVHLKEMLRQAAMHKGTSFLEIYQNCNVFNDGAFE
                     VFTEKASKKQEALFVEHGKPLVFGQNGELGIKLDGFKPVIVNHAEKGISTNDLWIHDM
                     FDRVKSGILARFFDNPANEGHFPRPFGVFYKEERSCYEDDLIQQVEAVISLKGKGDLN
                     KLLRGNEVWEIQ"
     gene            34006..34620
                     /locus_tag="PKY09_03140"
     CDS             34006..34620
                     /locus_tag="PKY09_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11879.1"
                     /translation="MIVFIGYLASAFLAISLLMSNAFKFRWYNLAGQIAFIVYGYFIN
                     SMPVVIANAILMVINIYQIIQLYKSKEAFDVIPVKESEPLAERFIERNKEEILEYFPQ
                     FEINRTSGFAIIVFRDMTAANIFIADVSEDGNATVRLNYTIPKYRDYKVGKFLFELEK
                     DFLIRNGLKKITYKNLTHAGHIRFINSLGFSKGDDSGNYFKILN"
     gene            complement(34627..36057)
                     /locus_tag="PKY09_03145"
     CDS             complement(34627..36057)
                     /locus_tag="PKY09_03145"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11880.1"
                     /translation="MKIRYKFLSILLVISTSNVFAWWNDILVYNNKPIAQHSMAAKSN
                     GTLYLAVPDSSAGMYSIQLLKSTNDGNTWLPVSNATGPMATAITKTKMVRTGSDSVYC
                     LYSTTAGVYLLNVETGIIGSFTSSSVADFDAAGSPAGNVIYLFVNEPINFTIKRYTTL
                     DGGLTWGGSTATVTSSGQTPKVYMSGTRLILNYYGPVNADPATSKIRAATYDESVPGT
                     IGATAFIDIVTNTAVKKKQFKTVYNYNTVWFFFTEGDTSQVIKCRISTDNGDNYGAEQ
                     IVAGNNGFSNYNFDACHYEFFGAWGCYLTYYNSNATLPPTPFPMMFTNASLNNPSVFS
                     TPVAYSDFNIVDTPSYHLPVISAFSGTTNYVGVAWVENSGNGSWVYFDRMSAATGINE
                     VKNDGFISGIIFNTSELNIHFVEQPKNKIIIQLSDVTGKIVYHTTSELKEKFIAIKTP
                     SLTAGAYLLQVSDGVHSATKKLILSK"
     gene            36178..38211
                     /locus_tag="PKY09_03150"
     CDS             36178..38211
                     /locus_tag="PKY09_03150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNB11881.1"
                     /translation="MRLLVTLLTVAIVVVACKNSKTPVQTHDELVQHIDSSVRPGEDF
                     FMFANGRWFAQNPIPASERSNGIFRTINDTINATILQICQTAASSTNDKGSLKQKIGD
                     YYSSGMDTIGIDKAGIAPLQEYFSEIDQMKTFNDVMKVAAHIHCISASPLFAFYVNSD
                     DMISSKNAVFLAQGGLGLPDRDYYFNTDERTKKVREAYPEHLKKMFELSGFDEKKAKD
                     AAADVYAVEFALAKVSRKMEDLRDPFKNYNKTSVKELQEKMNNVPMNEFFHGLGMNLP
                     DTVIVGQPEFFTYLNIATRQFGIEVWKNYLRWHLLRGLAPYLNTEIEKQNFSFYGTLL
                     NGVKEQRPRWKRVVEDTDGQLGELVGQIYVEQYLPKGTKEKLMEIGNAVKEVYRKRIA
                     GLDWMSDVTKAKAIAKLDAMIMKVGYPDKWKDMSALEVDKTSYAGNVIRANAWHFDYM
                     KNKYGKPVDRTEWSMTPQTYNAYYNPSNNEIVVPACNITVPGYEKTLADDAILYSIIG
                     GSTFGHEFTHGFDDQGSKYDQFGNLNNWWTSEDSIRFYAKTGAVVNQYSHYLVVDSLY
                     INGDMTQGENIADIGGIAMGLEAFKQTKQFKENQVIAGLNPMQRFFLGYAYAWMVNMR
                     PEALATRVLTDVHSPAKFRVIGPLVNMPEFYSTFGIKTGDKIFLPESERVHIW"
     gene            38239..38958
                     /locus_tag="PKY09_03155"
     CDS             38239..38958
                     /locus_tag="PKY09_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB11882.1"
                     /translation="MREESIKYYSPIIGRDFEMLIFGESGIPVIAFPTSMGRHYQNRD
                     FKLIESVAPFVENGFIKIYCPDSIDEQSWYNTHIHPADRVRNHILYDQMILEEVVRRA
                     QKETSHSKVITAGCSFGGYQAINFAFRHPEVIAHTFSMGGAFDIKMHLDGYYDDNVYF
                     NNPPDYLPDLDNEHLFQMGIVLGVGEHDFCIAQNKRLSEIMTKKGMAHWLDVRPGANH
                     DWPVWREMFPAYIEALQTAKV"
     gene            39367..40167
                     /locus_tag="PKY09_03160"
     CDS             39367..40167
                     /locus_tag="PKY09_03160"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNB11883.1"
                     /translation="MENQKEKSEIEDVREDVWRRFEKDNQRGKIFGGLIIICVGALLF
                     AREAGVYFPSWIFSWPMILIVIGIFLGVKHAFRNMAWMVLVLIGGLFLIRNSFPIFEY
                     RNYIWPIVIILLGLFIIIKPSNRFRRACRRGRFGTRHQQNQFGNYSNAPASGEDFLEA
                     VAVFGSIKKNIISKYFKGGEVSAVFGGAEINFMQADIQGRVELEVNQVFGGTKLIVPA
                     HWEIKSEIAAVLGSVEDKRPLRGTTTDEKKVLILRGTTVFGGIDINSY"
     gene            40204..40542
                     /locus_tag="PKY09_03165"
     CDS             40204..40542
                     /locus_tag="PKY09_03165"
                     /inference="COORDINATES: protein motif:HMM:NF025484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HNB11884.1"
                     /translation="MYFVAKVIYQIIIKGSKNNQFDEQLLIIKAQNVAEAYLKARAIG
                     AEKQHEFINDRKNNVKWQMIDVADIMPVESPEHGSVVYSQVIEEDSESFIDYIKRKAM
                     VLQTQSLLFA"
     gene            40549..41574
                     /locus_tag="PKY09_03170"
     CDS             40549..41574
                     /locus_tag="PKY09_03170"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB11885.1"
                     /translation="MIIEKIKNSIDQIIWWLCWYFFVAAVVHQNGFDWYLSFADTTVM
                     LLLLLFTSCSTYFAFNKFQKTGNRFFVILWNIALSLISVFILSLVLKNIYIDNLKYLA
                     FLDASIIVRFTWFFIMNLLVTVIYGLQANILEQKKLEAHNNDNINLMKDAELSGLRLQ
                     LQPHFLFNSLNSINALIGTEPAKARSMVLQLSEFLRGTLQRDNKKLITLQEEVNHLKI
                     YLEIEKVRFGHRLITQIEIPENCLNLLVPPLILQPIVENAIKFGLYDTTGQVTISLQV
                     SCNDSHLKIVVTNPFDPDTAASQKGTGFGLDAVQRRLYLLYARNDLLRTEKANNIFLT
                     QIDIPQI"
     gene            41571..42305
                     /locus_tag="PKY09_03175"
     CDS             41571..42305
                     /locus_tag="PKY09_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNB11886.1"
                     /translation="MIKAIVIDDEPLARSLVLEFLKAFPNIEVVQECNDGFEGLKAIQ
                     QFQPQLIFLDIQMPKINGFEMLELIENPPGVIFTTAFDNYAIKAFEAHAIDYLLKPFS
                     QERFYKSVTKFLETNSNAAVMDLVNAESNILPDEKNRIVVKDGNRITIIPVDDVIYIE
                     AYDDYVKVHIQNGFKLKKKTMSYYENTLDKSRFLRVHRSYIVNLSLITRIEQYEKTSH
                     IALLKNGQKIPLSKQGYARLKSVLGI"
     gene            complement(42307..42723)
                     /locus_tag="PKY09_03180"
     CDS             complement(42307..42723)
                     /locus_tag="PKY09_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11887.1"
                     /translation="MIKTCLLFVIIFYTVNCQAQWLKLKTRIAAEDNMYDSIVVVISD
                     ESGNVNRLSTSKESTLKLKLNNHYQVTYIKPDAVAYKFEVNTHLPVKLSKKNYSYSIN
                     IFLEKLNPVDAKAQKETACKTRMTIHFNKDKKAFSF"
     gene            complement(42720..43451)
                     /locus_tag="PKY09_03185"
     CDS             complement(42720..43451)
                     /locus_tag="PKY09_03185"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNB11888.1"
                     /translation="MTACKLSVNINKIATLRNARGGTIPNVVKAALDIERFGGEGITV
                     HPRPDERHIRFRDVYELKECVTTEFNIEGYPSDDFLKLVESVKPHQVTLVPDSPMVLT
                     SNAGWDAIREQAFLKKIIDRLKQNGMRVSVFMDTDLKQIDAAAATGADRIELYTERYA
                     SGFEKSPQQAIADYQKAAAHAAFLKMGVNAGHDLNLNNLNYLIKNIPQIVEVSIGHAL
                     ISDALYYGLQNTVQMYKHQIKSARE"
     gene            43518..44180
                     /locus_tag="PKY09_03190"
     CDS             43518..44180
                     /locus_tag="PKY09_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNB11889.1"
                     /translation="MNTKAYIKDFIPPLKSSDSVVRALQWMQAFNLNHLPVVDGVKYK
                     GIVTENDLKKIEDKEKTLAACDLEYQNVYIFETQYIYDALEFVTVKGLNIVPVISKEG
                     NYRGLLTLVDIIDCFAQNSSVKTPGGIIILNVDRKNYSMQEIARITESNGALILSSSV
                     NQLPDSEIFEVTLKVDKLDLSRILAGFYRMDYNVVASYQTSDLPTDLEDRYQAFMTYL
                     NV"
     gene            44173..45576
                     /locus_tag="PKY09_03195"
     CDS             44173..45576
                     /locus_tag="PKY09_03195"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNB11890.1"
                     /translation="MFKRALLINLFFAFCFSSFASDSLQVDTSTIYVRQIVVTGNKVT
                     RYSVIQRELAFSQGQTMKVAELDEKITKSRENLLNISLFNFVNINKQLIGNDVYVIID
                     VVERWYIFPVPIFEVVDRNFWEWWRDKNLKKANYGFYLNWENFRGRRENVKLLLRYGY
                     SQRQGFMYSIPSIDKSQNNGLVFTALQTRMREIPYKLENNKLVYFENGSEFLRKEYIA
                     NVRFIHRQGFNKTYTFTGGYQYNIISDTVLSLNRDYFVSGHKYESYPVISFEYKDDAR
                     DIKNYPLRGYFLSLEFTKYGFGISDNDPSITYIIAHFKKFWKLNNTWAAAFSFKGKLS
                     GNNEVPYYNQIALGYRGDLVRGYDPYVINGENFTLFKSGIKYAIVQPKSFNVPVGLPQ
                     SFTRIPYALYANLFFDTGYVRDKRFYQNNPLSNKWLYSYGAGLDFVTYYDLVLRGEFA
                     ITGNGKSGIFLHLTAPI"
     gene            45589..46470
                     /locus_tag="PKY09_03200"
     CDS             45589..46470
                     /locus_tag="PKY09_03200"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNB11891.1"
                     /translation="MTIAVYARNVNEKVLAALDVVWTQLRQNDVDVLLYGTLFRHLKK
                     LGNEMQNTHVFSTSSEIAGKADFLFSIGGDGTILDTIKLVKNSGIPVLGINTGRLGFL
                     SIVLPEAITEAINSILKGHYSLDKRAMLRLETDKEIFGDVPYALNELAIHKKDSSSMI
                     IIHAFLNGEYLNSYWADGLLIATPTGSTGYSLSCGGPVIAPASGNFVITPIAPHNLNV
                     RPIVVSDKNVVSLEVEGRNPYFMASLDSRSVTIDSSVQLAVRREDFSFNLVRFDNDNF
                     LQTLRNKLNWGLDARNW"
     gene            46537..47424
                     /locus_tag="PKY09_03205"
     CDS             46537..47424
                     /locus_tag="PKY09_03205"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNB11892.1"
                     /translation="MNDKYKKLLLVGLILIPFLGSAQVAKKRWKAYRIEYIAGLGVTN
                     FLGELGGANRVGTHAFRDLEFSMTRPAAMIGYRYKLSPTFAWCSKFSYGIVSGDDALT
                     KEHFRNNRNLSFKSNIFELSTNIEISFLQEQVGHRYKLRGVRGMRNVEISAYGFLGIG
                     VFHFNPKAKLDGQWYQLQPLGTEGQGVVASRSKYKRTQLCVPLGLGAKYAIDRQISVG
                     LELGLRYTFTDYIDDVSKSYYYDRGGSLVEILADRSLDDNSHVGEQRGDPRWNDAYVF
                     AMFNICYKIRTGRVNYPMF"
     gene            47471..48319
                     /locus_tag="PKY09_03210"
     CDS             47471..48319
                     /locus_tag="PKY09_03210"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNB11893.1"
                     /translation="MRKILILFFIVVVLQPQKSSAQSSELGLLLGVASYKGEINTHLF
                     NPNAFNPAAGLYYKRCLNSFWSFRLGLTYGQITGSDAIAKEDFNQYRNLSFRSDIWDA
                     SALFEFNFFPFQTASEHTSKVAPYLVAGVTFFHHNPQGFINGSWHDLQPLGTEGQGSG
                     LPNTTGRYKRVQFAFPMGGGIKFKLSNRFSLSVEAIARRTYTDYLDDVSTIYPNQNEL
                     RNTNGVLAAELSDRTIFAGTDINAKRQRGNASDKDWYMMGGFTINWTLSKKYTDKCKP
                     FRTKLR"
     gene            48667..>49778
                     /locus_tag="PKY09_03215"
     CDS             48667..>49778
                     /locus_tag="PKY09_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB11894.1"
                     /translation="MSETQNIEYKSSWRDEYLKWICGFANANGGVIFIGKDDAGKVVG
                     ISDAKKLMEEIPNKVKDTLGILVDVNLHKSKQGGFVEIVVEPYPYPVNYKGQYHIRSG
                     STKQELKGAALNKFLLQKKGKRWDGVPVPKVSVKDLKQETFEFFRKRALKSQRIDEDS
                     LTDSNEHLIENLQLKESEYLNRAAILLFHSSPEKFVTGAYIKIGYFQSDDDLKFQDEI
                     HGNLFEQIEKAMDLLFTKYIKAEISYEGINRVEKFEYPKEAVREALMNAIAHKDYSGG
                     VPIQISVYSDKVIFWNEGQLPDNWTVKTLLEKHASRPYNPDIANALFRSGYIESWGRG
                     TIKIIKECKQAGIPEPVFSYDSSDISVEFRKDIYNE"
BASE COUNT        15410 a         8827 c         9701 g        15840 t
ORIGIN      
        1 atgaaggcgc aacttttata gacataggag gttcttcttc gcgccagggc tccaaaatgt
       61 catctgtaga ggaggagtgg aacagaatcg gaaaagctat cacattaatt aaactgcatt
      121 ttcctgaggt attaatttct gtagatacag tttattcagt aattgcaaag cgtgcagttg
      181 attcaggtgc ctgcatgatt aatgatgttt ctgccggttt aattgacagt agaatgattg
      241 aaacagttgc tgcactaaaa gttccctatt tgatgatgca catgaaagga ctacctgaaa
      301 atatgcagga taatcctgtt tatgatgatg tgttgaaaga gataattgat tttttttcaa
      361 agcaattatc gttatgcaga aaagttggta ttactgacat tttaatagat ccgggttttg
      421 gatttggtaa aacaatagca cataattttg aactattagc caaacttaat ttactaaaga
      481 ttgtaggact tccagttgtg gctggtttat caagaaaggg aatgatctgg aaaacacttg
      541 atgtatctcc tgcagaggca ttaaatggca ctacagcgct taatatggta gcattaatga
      601 atggcacgag aattttaaga gtgcatgacg ttaaagaagc catgcagtgc ataaaacttt
      661 tcaataaact agatgctgca aattaacaag agtactattt tagaatagga ggagaaaagg
      721 tcaactaaac caactctgca atgacggtta caccaccttt ggttttctga ttgagttcaa
      781 cattaatctt tgctgtcagt ggcaaataaa tgtcaacacg tgagccaaat ttaatgaacc
      841 ccatttcggc accttgtttt accaggtcgt tttctcgtgg atagtaaaca attcttttgg
      901 ctaatgctcc tgcaatctgt cgtaataaaa tagttttgtt ggaatccttt tcaacaacaa
      961 tggtagaacg ttcattttct gttgaagctt tcgggtgcca agcaactaaa tattttcctt
     1021 tatgatagcg taaaaatttt attttaccac tcataggata ccagttggca tgcacattta
     1081 taggcgacat aaaaatagaa acctgaatac gtttgtcttt atagtattca ctttcttcag
     1141 tgcgttcaat tactacaact ttaccgtcag caggggcaat gacatagttt tcattgcggg
     1201 taatatttct ttttgggtag cggaaaaact gaagtatcag taaaaagaaa actagggtta
     1261 cgattaaaac taaaactgtt atccaatcgt gttctgcacg aaaattaaga acaagaaaat
     1321 tgataaggag gacagtaatg aatgtccccc acacccaatt ttttccttca cggtgaatag
     1381 tcatttttag tggattagta tgcggtattt ttagtagtga ccgcgagatt taattcctaa
     1441 ttcaattttt cccagtgcat aaatatatgc agcaatgcgc atagttgttt tatgcttaac
     1501 agaggtgttc caaacattgt ggaaagcttg tttcatccaa tgatcggctt ttgtgtttac
     1561 ttcatcttct gtcatatata atcctgaacg gttttgaccc cattcaaaat aggatacagt
     1621 tacaccacca ccatttgcaa gaatatccgg aattacaact gttccttttt tgctcagaat
     1681 gtgatctgcc tcatcagtag ttggtccatt ggcaccttca acaatcaatt ttgctttaat
     1741 ctttgcagcg ttttcgtcag taatctgatt ttccattgca catggtgcta acacatcaat
     1801 attaagcgtt aataactctt cgttagagat aagtttacct cctttaaatc ctttaataac
     1861 acctttattg gagtttctgt attttattgc cttgtcaatg tcaataccat caggattata
     1921 gaaagctgca gtatgatcgg atatggcaac aatttttaag cctttagctt catagtgttt
     1981 tgctgtgata gagccaacat ttccaaagcc ttgtacagca gcacggcatt tagcaggatt
     2041 caaccccatt ttagccatag cttccattgt ggcagtcata actcctcgac ctgttgcttc
     2101 tgagcgtccc aatgagccac caagatgaat aggttttcct gtaactacac cgggagtaaa
     2161 acctcctttt aacttactgt attcatcaac aatccatgcc atttcctgag ggcctgtgtt
     2221 catgtcaggt gctggaatat ccttgtccac accaaataca tcaaccatag catttgtata
     2281 ggctcttgtc aaacgctcta attccccttt agacatagtc gaaggatcac aagtaattcc
     2341 accttttgca ccaccataag gtatatcagc aactgcacat ttaaaggtca tccatgccgc
     2401 aagtgccatc acttcatttt cgtttacata agtgctgtaa cgtactccac cttttgaggg
     2461 tcctaacgct gtgctgtgaa caactctgaa accttcaaat acttttgttt ttccattatc
     2521 cattttaacc ggcatactgc aataaacaag cttgctcggc tttttcataa tttcataaac
     2581 ttcagagtct aatttcatga tttttgctgc tacatctaag cgcttaatca tttcttcgaa
     2641 tgggttttcc tcttcgtgat tttgttttgc ttttttacta tcagccatgt cttttttaag
     2701 attgattggg aacgcaaaag taggaaagat ttatgtgttt gcaaaccatg aggaaaggct
     2761 gttttctttt attggtttta catgagataa atcatgttta tcgtatgcag cagtatattt
     2821 aaaacattca attactgtct gaaaattata gccattaaga agcattgtga cataccgttt
     2881 acaatgcacg gttctatcaa tgaaaaagat gttatttaaa tttattgaat aatgttcact
     2941 tcaggttcaa tgtggacacc gaaatggttg taaacagatt ctttaatttt tagtgcaagg
     3001 ttatagattt cagcaccggt ggcattgccg tagtttacca gcactaaagc ttgattttta
     3061 tgacatcctg catcaccttc tctaaacccc ttccacccac attgctcaat aagccaccct
     3121 gctgcaagtt tgattttgtt ttcacctgcc ggataatgtg gaatgtttgg atgttgttgt
     3181 ttgatttttt caaattcctt gtcagtaatt tcaggattct tgaaaaaact accggcattg
     3241 ccaatcattg ccgggtcggg tagtttactt tttctgatgg caattactgc atcacttatg
     3301 gctttaacac ttagtgttgt aatccccagt ttttccagtt ccgactgaat ggccccatat
     3361 tcggttttaa actgaggctg tttctttaat tccagaataa tggcggtgat aatgtattta
     3421 ccttttacag cgtttttgaa aacactattc ctgtatccga attcacattc ttgattagaa
     3481 aaaacttttt tttctgcact atcaattgcc atagcttcaa cagctacgag tgagtttttt
     3541 atttcaacac cataggcacc aatattctga ataggtgcag ccccggcagt acccggtata
     3601 agcgaaagat tttcgaggcc accaaggttt ttttctaccg accacattac aacattgtgc
     3661 catacctcgc ctgcaccaac ccgcaccttt acataggttt cattctcttc aactacgcta
     3721 atgccgttga ggttgttttt taaaactata ccatcaaaat ctttaaaaaa aagcatattg
     3781 ctgccgccac ccatgaccaa taaaggcagt gctgtttttt tatgctgaag aagaaagctt
     3841 tttaattcat cctcactgtt tatttcaata aaatagcgcg cttttgcttc aatgccaaag
     3901 gaatttaaat tctttaatga aacattttca cgcataggca tcagttgatg atgaatttac
     3961 caatagcaac aacttcatta gccgacatga ttcttacata atacattccg ggtatcatac
     4021 cttgattttc tatgctgaag gtttgttttt ttccgttatt gccaatattg ctataatgta
     4081 tatttaccat tcttcctgct acatcaaaaa agtataattt aaagtctttt gattctgtag
     4141 ttgtaatgct taagaaagcc ttttcagtaa agggattcgg gaaaatttta atttctttta
     4201 tttccgctga aagactattg attgccgtag cttcatcaac aaatacacta tcgcattgca
     4261 tgcagttgtt gaagtcctgt gcacaaaata aataatatcc catgcccaga ttggttgcag
     4321 cggaatcagc aatacctaat gtaggataca aataatcata cggtggtgtg ccacctgttg
     4381 cagttgctac aatgctgcca tcagtacaac cggggcacga agcatctgct actgtaaaac
     4441 ttattacaat agtgtcaggt tcaacaaaag taaaagagtc aactgctgca cacagattag
     4501 catctgtaac agtaaccgtg taggtgcctg cacaggtatt aaaaatagaa tcaaaggttg
     4561 caccgttcga ccataaataa gtatatccgg gagttccacc tgaaggtatt gcacgaacaa
     4621 agccatcaca gtttccatta cacgttattc ccaggaaatt attcccaata agtattggtg
     4681 aaggttcaaa aatcaggaca gaatcggtga cagaacaatt gttagtgtca gtaatagtat
     4741 aggcagtgta gccaaagcac aaattagtat tggttgagtc tgtttgtgga ggcactgtat
     4801 tccataaata agacaaagtg ccattaccgg ttgcagtgat agtagaaata ccgttacata
     4861 aattggcgca ggtagcatct gtatgcgagg cagaataagt tattgctggt gggtcaataa
     4921 tggtaacaga gtctgcacca atacatccta cagagtctgt aattgtgatg taatatactc
     4981 cggcacataa actgtcaata gatagtgatg ttgtatctcc agttgaccat tgccattgaa
     5041 atggtgctac acctgatgga tttattgaag ctaatccgtt acattgccct gcacaagttg
     5101 gtgctgttgc aacgggattt aataaaacag gttgactgat aatttgtact gaatctgtta
     5161 caatacatcc aagactatct gtaatactta cagtataaag tccttgacaa agtgaatcga
     5221 ttgaagcaac tgtatcgcca gttgaccata tataacttgt tggattgatg cctgtaatta
     5281 ctgcgcttac tgcgccagtg cacacaccgt tacatcctgc aaattgtgtt gtgaaagtaa
     5341 ccggaagtgg aggagcaggg ttaagtgaaa cagaatctgt tactgtacag ccaatactgt
     5401 cactaatggt aatggtataa acacctgtgc aaagtgaatc tattgtgctt gttgaatcac
     5461 cgtttgacca ttgaaaaata aatgggatag ttccggttga tgttgcagtt aacaaaccat
     5521 tgcaagaagt gacacatgca ggcggttgcg atgcaaaaga aatcagatta gccacaggct
     5581 ctgtaatcac aacggagtct gttacagcac agagattact gtcagtaaca gtaacaaaat
     5641 atgttcctgc acataaattt gtagcagtat cagtagtttg aacaggtgtt gtgttccaca
     5701 aataagtaaa tggtgaagtt ccgctcgaaa caacagatgc agaaccgtta caacttccaa
     5761 agcatgttgt agctgcagtg ctgaattgta atgttgcagc tgtaggattt ataatctcta
     5821 ttgaatcagt tgccatgcaa cctgtactat cagtaacggt aatgtagtaa acacctgcac
     5881 aaagtcccgt tgctgtatcg gtttgttgtg caggaatggt attccacaaa tattgaaaag
     5941 gtgcattccc tgttgatact actgcggcaa tgccatcaca gttattgtaa catgtcgggt
     6001 ttgacaaagt aagagaaagt gtgtttggta ccggattggt tatgataaca gaatctgtta
     6061 ccatgcagcc tgcagagtca ataagtacaa cagaataaat gccttcacaa agccctatgg
     6121 cagtttgtgt gttttgtgga ggaatagtat tccagttgta agtaacagta ccttgtccat
     6181 tgtttgatgc taatgcattg ccatcgcaca gaccaaagca tgtagggtta ttagaagaaa
     6241 agctaactgt aaattgtgca ggttctgtaa tggttacagg ttgtgtaatg ctgcaaccga
     6301 tattgtcagt aattgtaact gtataatttc ctgcacaaag gtcagctgct attgaatcag
     6361 tttgaacagg agtagttgac cattgatagg taaatggggc attacctgtt gctgtaactg
     6421 ttgccgttcc attacactga ccaaaacaag atgtttgtgt tgtagaaata gaagcattgg
     6481 tcaagcaggt tgttctgtat ttggcaatat aggttgccgg atttcctcct gccatagtga
     6541 aagcaccgcc aacatgcatt tcattaccat aattcaataa tgcatctacc tgattgtttg
     6601 ttccaccact gaggtcggac caggtggtgc catcataagc tgcaatattt gaaacagcat
     6661 ttccgcctgc aagagaaaaa ttaccaccta cataaagtgt attaaataaa tatccaagtg
     6721 atttaacttt agcttgccct cctgatatac ctcctggaat tgctaaccat gctgcaccat
     6781 tccattttgc aacgctattg gaagaaacac cacctgcaga actaaaggtt ccaccgcaaa
     6841 tcaactcacc gtttacttct ttaaaagctg ttacccgcat agataatcca ctaacgccac
     6901 cattcaggtc aaaccagttt gtaccattcc atcgtgctat tccgcttaca gctacaccac
     6961 ccgctatgcc aaactgccca cctgcaatta aatcaccgtt ataaaatgtc aatgcttcaa
     7021 catagttaga tccacttcct atgcctgagc ctaatgcatt ccatgttgtg ccattccatt
     7081 tagcaattcg ttcggtgctt ataccaccag cggtagtaaa tattccaccg gcataaatgt
     7141 ttccgttagc atctcctgta agtgcataaa tctcatcatt acagcctgta cctaatggtt
     7201 gccattgtgt gccatcccat tttgcaatac gtgttatcgg atttattcct gaagataaaa
     7261 acgaaccggc agcgtaaagt gtattgttac tatgatcata atacagtgcg aatacgttgc
     7321 tgtcaaatcc atcaccaatt gcagtgtaac ttgttccatt ccatgatgcc actcttatgg
     7381 cagtaacacc actggcatcg gtaaaccggc caccgatgta gagaatgttg ttgacagtgt
     7441 ccttggtcat ggcataaaca ttgttattga caccggaacc aacgggtaac caaaattgtg
     7501 cttgtacttt ttgcgatgtg caaataaaaa agcaaatgat aaaacttaaa aaagttaaat
     7561 tgaattgaat tcttcttctc atgattctcc ttaaaattta ttttatagtg aaaaattatt
     7621 gtgcaataag ttctttaata ataattgtca ttttttgttc tgctgcattg gcaactttca
     7681 aaacttcttc gtgagtgata aactccggtt catcgcttcc acctaagtca gtgataatag
     7741 agattgcaaa aaccggcagg ttcatgtgac gtgcaactat gttttcagga actgttgaca
     7801 tgcccacaca atcggcacca ataaacttaa tgtatttgta ttctgcacgt gtttcaaacg
     7861 ttggccctgt aacggcagca taaactcctg tatggcaggt aaagccatgt ttgtctgcaa
     7921 tggcttttgc atttttaatc atagttttat cataaggctc gcccatatct ggaaagcgtg
     7981 ggcctaaatc atcattgttt ggtcctatca aaggattggt gcccatcaga ttaatatggt
     8041 catccaatat catcaaatcg cctactttat attttgggtt aacaccacca cttgcattgg
     8101 agagaataag attttttatt ccaagaaatt tcatcactct gacaggcatt gcaacttcct
     8161 gcatggagta tccttcgtaa aaatggaagc gaccttgcat cgctactact tttactccgt
     8221 ttaatgttcc gaaaataagt ttgttgccat gtccttttac tgttgacacc gggaatcccg
     8281 gaatgtctga atagtttaat tcttttacaa tgtcaatctc tttaactaat cctccaagac
     8341 ccgatcctaa aataatgcct gtttccgggc taaagcccgt agttttgctg ataaattcag
     8401 ctgttgcttt gattttttcc aacatacgct tttactgtat ctaaaaattt tatttcgttt
     8461 tcattgatga caactgttac tggtattgaa tatacaggaa gatttttagt gagtggtttt
     8521 aattcactgc tgttgaattt tacagcatct ttttctgtaa ctacaattat ttttttttca
     8581 gaggctaaat tattgaaaat ggcaataata ttttcaagat cttttgcaca ataattatgg
     8641 tgatctttaa aggaaatatt tttgattttt ttgctatgcg tacttagaaa accgagaaga
     8701 ctattattgg atgcaattcc cgttattaac aaaatttcat actccgttaa gtctcttaaa
     8761 ttcagctttt catcactatt aagcgactga aggttgtcat aactaaacga actgaaaaaa
     8821 acaggttttg atggtaattg actgataata gatttttttt gttcaactgt tattgctgtt
     8881 gggcatttgg tgactattat cacatctgct cttgatgcag acgatgcaga ttcccgaaga
     8941 ttgcctgcag gtagtaagca atcatttata aaaaggtcat tgtattgtgt cagcaaaata
     9001 ttcattcctg cttttacact gcggtgttga aatgcatcgt caagtaagat tacttctgta
     9061 ttcggataat gttttaaaag ataatcaatg ccttcacttc ttttttcgct gacgatgaaa
     9121 ggcacaattg gaaatttacg tttaatctgt aatggttcat caccggtatc attgggctgg
     9181 ctgtcaacat gtacttcaac aatgcctgaa gtgttacgac catacccacg tgaaacaaca
     9241 gctgttttat aattactcaa tgcagaaagt agatattctg tatgtggtgt tttacctgtg
     9301 ccaccggtgt ttaggtttcc aatacatatt acaggaatat ctacagtccg gctttttagc
     9361 agaccaacat caaacaataa attacgcaat aaaacaacca ttccgtacag ccacgagaaa
     9421 ggtaggagta atatgcgtaa tgacttcatt gaacaaaggt attgatagta aagagtttta
     9481 taaaaataag aaagcctacc cgattaaagg ataggcttct ttatattgaa aaatattatt
     9541 atgcctgtgc tacaggagta acgtaaacgt atgacttgtt atcagttttt ttcttgaaaa
     9601 caactttacc atcaaccaaa gcaaaaaggg tatgatcttt tcccatgccc acaccttcac
     9661 cggcattgtg tttggtgcca cgctgacgaa caatgatgtt gcctgcaata gcatgctgcc
     9721 cgccatatat tttaattccg agacgtttac tgtgcgactc gcggttgttt tttactttac
     9781 cttcaccttt tttatgtgcc atgactaaat aagtttagtt aattatgcgt taatggaatt
     9841 gatttcaatt ttactcatat actgacgatg accattcatc ttcttgtatc cttttcttct
     9901 ctttttctta aacaccagaa ctttgtcacc ttttacatgg ctgatgattt ttgcagagac
     9961 acttgcgttt gataaagaag gcttgccaac ttttatactg ccaccattat ctactaacaa
    10021 aacatcgttg aattttactt cttcgccttc agcaccttct aaacggtgta caaataattg
    10081 ctgcttttct tcaaccttaa actgttgtcc taatatgttt actattgcgt acatgattaa
    10141 tgtgttttta caaacggacg gcaaaagtag ttttttttac tatattttca aatcctttgc
    10201 taaatttatt ttcaacttca aatatttgat taatcggccg ggaaaagact gattatacgt
    10261 aattaaagga taataaattc attgattatc aatataatat aaaaatctat tctccggttt
    10321 tgactaattt tatacttgaa acagtgcctt tatcagagac taattttaca agataaacgc
    10381 cttttttgaa actcttgcca ataaaaatct gctcattttt ttgaactttt agtgtttcta
    10441 ccattttccc ggtaatatct gtaatggtta agttaccacc attttcgcca atgtgcagtc
    10501 tcgactcatt tttaaacgga ttagggcttg atgacaaagt atttgcagtt gctgataatg
    10561 ttgatatgcc aacagttccg tttacttcaa atttatcaaa accaccttca accacatgtc
    10621 ctgacattgc ttggtcggca gtgtaaacac taatctgcat ggtattggtg acaggaatca
    10681 aaccatttaa gttaaatgac tgtgaaaccc atgttcccat tcccggagtt gaagcacgaa
    10741 ctgtttctaa aacaacattg gtagtgccat tagaaattcg aattatcaaa ctatcgttgg
    10801 ggtttgaatt tccaccggca ttataaaacc agcgtgaata atttatagtt ggatttgtca
    10861 gatatgtagg gtcaaaaact ggagaagtca aaagtgtata gccgtcatca acatcgtttg
    10921 atcctgaaga agatgcatta ggattattat ctgttacata ggcctgattc aaacaatctg
    10981 tttgatcatc ggcatcagga ttggcaaagc ccggtccgaa aacggcagga gttccgtgag
    11041 gcacacctct ttcccaaaga ttttgagtag tacctgttat tgtccatccg aagtcaaaag
    11101 aaaagtcatc ataataacct ttgttcaagg taattaccaa tggtgcaggg ttaagcataa
    11161 tgtttgagtt gaagcatgta gtgcgatgac cccaacttcc ggctacaacc tgatagttgc
    11221 cgactaccat tgaaggaatt gtaaaattac ctgaggcatc agcagttgta ttatacacca
    11281 atgcactatc ctgaatggtc accgttgcat tgggtattgg atttcctgtt ccagcttcaa
    11341 ctacctgtcc acttaccgat gtagaagcaa gtggtgataa ctgaacatca agttgcgtta
    11401 cattgccatt ggtgagcgat actccattaa ttgtcttggt gaagtagcca gccgcagaaa
    11461 cttcaacatt ataactacct gcagtagctg ttcctgtttt atattctccg tttgcttttg
    11521 tgtttttagt aatgtttgta gaaagaactt ttacggttgc attaaaaata ggattgccgg
    11581 taactgagtc tgtcaccaca ccttcaagat agcaggcacg ctgataagta ggtcctaaaa
    11641 caaaaagtcc gttgttgatg tcagatacta caagattacc tgagggcaga taaggataaa
    11701 cgccccaatc cccactaaaa ccatcacctg caccttgtgt ataggtgtca taccatccaa
    11761 cgttaaccat gttttgtggt cgagtaacat cagtaatcac tacgccatct ttataccagg
    11821 aagtaactgc ataatcagca ccattttttt gtataatgtg tgtattgtgt acaatagatc
    11881 cggagccagg agtgatttga attctgtcca tttcaattat gttggccgga tttgaaatat
    11941 cataagctgt cagataggag tcagaaactt catcagtagt aaacaaatac tgactattgg
    12001 tagaaagcca tgcattgtga gtaaactgcc cgggtgttac ttttattccc aatggaatgg
    12061 gattggcttt attagttaca tcataaattg taaaatcacc attataaata tggcatccga
    12121 aataataatt tcctctggca taaccgtcat gtacgtaggc accgggtgta tgtccaacat
    12181 aattcgggct tagcgggttg gtagcaatat cagcaatgat tgttgctccc tgaaataaat
    12241 ttgagccatg cagataaaca tagccatctt caatatgcag tgagtgaatg ttatccaact
    12301 ggccactgat ggcgccatta cctgtccagt caattgcagg tgctgcatta ggaagttgtg
    12361 agaggtcaac tatttgtagt ccaccaccac cttcagtagt tacgtatgca taatgatcgt
    12421 aagtctttac ttcgcgccat tgagattgtg gtccgggaac taaaattact tctgtaggat
    12481 ttgccggatt gctgacatca acaatagaaa gtccggattc tgagcctacg agtgcatatt
    12541 cttcgcccga aggagatacc catccgccaa tattagcaag gtcgtcattg ggataaggca
    12601 atgtgccgag taatgaaatg ttgaagtttt gtgcattaac tgttgaagca aagcttgcag
    12661 ctaaaaggag tgtgaagaat tttttcatgt ttatttttga aaagaccgac aaagctatta
    12721 aaatagccat tcaaagggtt aaatggtaac attatcttaa actttaatta acagatgaaa
    12781 atgttagggt acagatgcgt aaaatttgag gagaccttaa aagttaaagt caacattgct
    12841 taaacccggg agcacaactc aggttaatta ccaggctttt ccaaactcat tcaaatagtc
    12901 taaatgttat ttcaagcaga ctcaaagtag aacacttggt gctattgaaa cattattttt
    12961 tttgtcatta tatttttctc tgattgcaac ctgacaaaat aaacaccttt gcttagaccc
    13021 gataggttga atgaatatgc agcgagttgc tctgaagtgt tgaaatagta tgttcttaat
    13081 gtttcaccac ttatgttgtg tatggaacat accgtattac caaatttatt ttcaggaacc
    13141 agataaaaga atccgctggc ggggtttgga taaatatcaa agtttaaatt aagttcagat
    13201 tccggagtat gactttgaac acctaatact aaaggtaaat tactgtatcg aatcaaataa
    13261 ccaccaatgc gataagaagg attgtagcca tcattacaat tggcacaggt aactcccgac
    13321 acacatgccg ggttgttggg atgacatata tcctgatagc tggtttggaa aatatattcg
    13381 aagttaaaag gtgcttgcga taaatatggg gttaaatcat agaaataagg tttaggaatt
    13441 gttccggggc accaacctgc gcggttgtat tgccaggttc cttgttgtcc tgtacagcca
    13501 tcaggattgg ggttgcagtc tgtccataaa tcctgagtaa atgtggtcac attgtttatt
    13561 tttaaatcgt gaatagcatg gtagaactcc gctgcattgc ctgtgttgtt gctaccccag
    13621 ccatgtcctg tggttactaa tctgaacgta gcctttgata cattagaata gggttttatg
    13681 acaacagtat ccataggttg taaattgagc gggttaccaa agttatagtt acctttccaa
    13741 agttgttcaa tggttgagta tagaaatgca ggcggtgaag cgtaataatc aaagtcaaga
    13801 tttatttgcc atcctcccgt tccccaggtt tcaatgtaca tacgcagttc aatttctcct
    13861 tgtaataccg atgcataatc ggtaacatca atctgatggt tgcacgcaac acgatagggt
    13921 gttatataac gaaacagctc catccattca ccgttaggag ctttaaattc aacccatgct
    13981 aaacgatccc agtcatcgca tcctccgggc actgcagggc agctgatgtg caggttggca
    14041 acaatgctat catatacgcc tactgattgt ggcagcgtat aggtgccata aaaccattgc
    14101 gagccaattg tgcccatatc aatgacaccg ttggtgatgg tttgtgcatt ttgtgttgca
    14161 aaggtattgg tgacattcag gttaatggta tccacatcgg tgtttccgtt gttgcctgtt
    14221 gctgtaacgt aaatggtgta gttgccataa gcaggtggtg tccaccaaac ctgatagtcg
    14281 ccatcattgt aaatggtgtt taatactact ccgttgatat tgtattctac cgaggcaatg
    14341 gataataaag taggttctgc aatgctggca ttggtatgca aaagataggg gtaaagttgc
    14401 ggcatttcca agtcataatc accggtaagc ttgctgctta cagcaggaaa gtaggtagtt
    14461 aaatcaatca catcaaaaga tagttgtgca cttgccgggt agtataatgc attacctgcc
    14521 tgatcggctt gcaccattac agtgcctgtt gcgcctgtca aagtcaaagt atcaccattt
    14581 atagttgcag gacctgatac aatagagtag gagataggca gtcctgatgt tgtggtacct
    14641 gttaatgtaa ttggcgttga aacggtagat tgcttaggta ccggattgag tgatataatt
    14701 tgattgtttt gaccggtagc accgcagctc aaatcctgaa atacgttgag ttctgcacac
    14761 atcacatgaa tgccggaata gcttgagtcg aaaactaacc tgatgtattg tgcattaacg
    14821 gcaccaaaat aaattgcagc agtctgttga gcaccgctgt tgggatttgg ataaattaaa
    14881 acaccagcag cttgtggact accccatgtg atgctatcgt tactcagata aacatgaaaa
    14941 ttttttactc ttccaccggt gttgttgtca cgtgttaaaa aactaaaacc attaacagga
    15001 taaacagcac ctaggttgat ttgaatttca tgagggtatg gagtagttat tccctgccat
    15061 ttggtatgcc agaaggtagc agtgtcgttg tcaatacaat gtgtagcatg accattattt
    15121 gggccttcgc ctacaagttc ttcgctgtca acataactta ctgtccattg agtttttgca
    15181 atggatgtag tatcgcatgc ttgcgaaata tttaatacaa aaacaccgat aagaagcagg
    15241 tatatttttt tcatcattta attttattta ttgatttttt cgataattat tgacaacaaa
    15301 agtaattata atagatgacc ttttccactt aacttttcaa cttcatcaaa caactaaaca
    15361 tagtgtcgga ttgatgtttg tatccatgga aaaatgtttg ctccacaata ttaagattgc
    15421 aatgttttgc aatacgcttg gtattttctt cgttttccat tccaaaggca gagcaagtca
    15481 agtacagaaa cagtccatta ttttttagat gttttgttgc atttttcgta atagtaaatt
    15541 gaaggtttgc ataatcctta attttatttt tatcgaaatt ccaaaagttt tccggagtgc
    15601 gactccatgt gccggagcca ctacagggta catcggcaat aattacatca aatttttcat
    15661 tgaagtttaa gtcttttgta agatcggcct gcatcgtttt ataatttctg acagcagctt
    15721 tttttaatct ttcttctgca ttttgcagtg atgaagtgcg taggtcggta agtaaaaaag
    15781 cagccttttg aaaacggtcg gccaataaca atgtcttgcc tcctgatcca ctgcaacaat
    15841 cccacatacg ctgcccgcag ttgtcgggaa gcaacgatag tgtttgttgt gaagataagt
    15901 cctgtatttc aaaatgtcct ttcagataag attcaagtgc agtaagtggt atggcaggag
    15961 caaaaccaag tgcattttca gtgctttgaa aggagataat ttcttttaac tttaattcat
    16021 ctaatacaga tttttcaaaa cctttgcgta ttcttatcca cactaatggt tgttgaaaca
    16081 ttgatgcagc aaatgcttct ttatctattt cttttgatag taaatctaag aatgaaaagg
    16141 ctacatcagg agttattccg tcttgttttc ggataaattc cagcctttca gaaaggctgt
    16201 ttgattcatt ttttgcaatc tggtattttt cagataaata gtcacttaat aaattttttt
    16261 cattagtaat agaaaagctc aatgccaatt gttcttcaac acttctattg ggcagaagta
    16321 aagcatagcg atagtagtca tagcatcctg atgaaattat ttttctgtcg cgactgccaa
    16381 gttgcttgtt ttgcctgaaa tggtttttca gataatgatg caagggtaca gaaaaatcaa
    16441 aaccggcaat gatgccggtt atatgattaa gtaatgtttg ttgacgcatg aatcagattt
    16501 ccattttcat ctgtactttt tgtgtaggtg tttttacctg ttcggcaggg cttttttctt
    16561 ttggaaactc aaagagttca ggttcacttt ccttgtcgtt tttttttgaa ggctcagaac
    16621 tttttactgc agaagcttct gattttcctt tgccaacttc tgtgattttg tgaacgggaa
    16681 atttggtgag aatatttcca acagcagaac ggttttttat cagcatctcg ttaaagttga
    16741 cattaaacat tgtgtttttt aatttaggac gtggcttcag cacaatttga atggttggcg
    16801 cattgtcagt tcccgaaata ccgagataga taatctttga gttttctgtt ccttttgtaa
    16861 gatcataaat tttttcgcgc gtgatgcctc caatcgaaaa acgtttgata aacaaatcac
    16921 ctcgcgcacc atctctatat accatgttgt aaacggtgtt ttcatcattc ttctcaaaca
    16981 ctttgatgaa acgaatgtct ttaccataat aacttttatc agatattcgg ttcactacaa
    17041 attttccatc accggttatg gccaaaacat catcaatatc tgaacattca ctaacaagtt
    17101 catctttttt cagaccgtag ccagcaaatc cctcttcacg atttacgtat aatttggcat
    17161 tattgataac aacttttcta acttcaatct gctcaaatcc ttcttcgctg attacagttt
    17221 tacgctcttt gcctttaccg tattttttaa gtaaatcttt aaaataagca atagcataag
    17281 gtgtcagatt tttaagatta tcttttacga cttttatttt atcgttaagc gctttaatat
    17341 gttcgtcagc tttcttagca tcaaattttg aaatgcgttt gatttttatt tcagtaagtt
    17401 ttacaatatc atcttcggtg acttcgcgct tcagtaattt cttaaacgga ttcagtcctt
    17461 tgtcaattgc ctgaattact gcttcccagg tttcgcattc ttctattttt cgataaatac
    17521 ggttttcgat aaatattttt tcaagagatg aaaaatgcca atcttccatc aactcgctca
    17581 actcaatttc caattcacgt ttcaataatt ccagtgtatt gtaagttgat tctttcagaa
    17641 tgtcattcac attcagaaat tccggtttgc catcacgaat cacgcaactg ttgggagcaa
    17701 tgctcacttc acaatcagta aatgcataaa gtgcttctat tgttttttca atatcctcac
    17761 tggagtccgg atgtagataa atagctattt caacttctgc agcagtgttg tcttcaacct
    17821 tttttacttt gatttttcct ttgtcatttg ccgaaaggat agagtcaatt agcgaagagg
    17881 ttgttgtgcc aaaaggaatc tgtttaatca gcaatgtctt tttgtctttc tgctcgatga
    17941 cagcacgtac ctttattcta ccaccacgca atccttcatt gtaattcctg aaatcggcaa
    18001 tgccaccggt ggcaaaatcg ggaaggatgt ttgttttctt attacgtaaa acatcaatag
    18061 agccttgtat caactcacaa aaattgtgtg gtaagatttt acaggccaga ccaacggcaa
    18121 ttccttcaat gcctgatgcc aacagcaatg gaaattttac aggaagagaa accggttctt
    18181 tatttctacc atcatatgaa agctgccaaa cagtagtctt tggattataa accacatcta
    18241 atgcaaactt tgacaggcgt gcttcaatat aacgtgaagc agcagcggca tcaccggtgt
    18301 aaatatttcc ccagttaccc tgcatgtcaa ttagtaaatc gcgctgacca atctgaatca
    18361 gtgcatcacc aattgaagca tcaccatgag ggtgatattt catggtgtta ccaataatgt
    18421 tggctacttt gttgtatctg ccatcttcca tttcccacat gctgtgcagg attctgcgtt
    18481 gaacaggttt tagtccgtca tcgagatagg gaacagcacg ttcaagaatt acatacgagg
    18541 catagtctaa aaaccaggtc tgatacatgt ttgaaatgtg tgttgccggt tgttcgacaa
    18601 tagtgtttac aggtgatttt acaggtttaa tatttttttt gctcattagt tacttttttg
    18661 tgtccggtga cggtgaaata aatcaaatta atttcaatta tgcttctgca atttcaggtt
    18721 ctaactcttc ttctagtttc agattgccga taataaattc ctgtctttcc ggagtgtttt
    18781 tacccatgta atattctaac aaatgctgaa cggaaccttc gccttcaata cgcacaggtt
    18841 cgaggcgcat tttacgacca atgaaatttt taaattcatc aggtgaaatt tctccaagac
    18901 ctttaaagcg ggttatttca ggtttgcttc ccaatttttc tattgcagca acacgctcct
    18961 gctcgctata gcaataaatt gtttcttttt tattccttac tctgaaaagc ggtgtttgta
    19021 aaatataaac gtgattattt ttcaccaact caggaaaaaa ttgcaaaaag aaagtgagta
    19081 acaacaatcg gatatgcatt ccgtcaacat cggcatcggt ggcaattacg atacggttgt
    19141 agcgtaattc gtcaagtccg tcttcaatgt ttaatgcatt ttgcattaga ttaaactctt
    19201 cgttttcgta aacaattttt ttagtcagac caaaacaatt caaaggtttt ccttttaaac
    19261 tgaaaacagc ttgtgtgttt acatcacgag atatagttat agaaccactt gccgagtcgc
    19321 cctctgtaat aaacagcgtt gattcaaatc tttcaggcga cttggagtta aggtgtgtac
    19381 ggcaatcacg taatttttta ttgtgcagat ttgccttttt tgcacgttcg ttagcaagtt
    19441 tttttatacc agcaatttct tttctttcac gctcgctttg ctgaattttt ttcaaccaga
    19501 tttcggcagt ttcaggatgc ttgtgaaggt aattgtcgag gttggtttta acgaagtcgt
    19561 taatccagtt gcgcaatgaa ggaccgttag gataagtatt ttgtgagcct aattttgttt
    19621 ttgtctgcga ctcgaaaacc ggctcctgaa tacgaatgct tacagcagct tcaatacctt
    19681 cgcgcacatc acttgggtcg aactcttttt tgtaaaactc acgtactgtc ttaactactg
    19741 cttctctaaa tgctgcaagg tgagttccgc cttgtgttgt gtgttgtccg tttacaaatg
    19801 aaaaatattc ttcgccataa cttggcgtat gggtaaaggc aatttctatg tcattgtctt
    19861 ttaaatgcac agcaggatag agtacttctt cactcaggtt tgctttcaac aagtcaagca
    19921 atccgttttg cgaaaagaat tctttgccgt tgaagtttaa ccttaaacct acattcagaa
    19981 aagcgtaatt gttcaacatt ctttcaataa actcactgtt gaatttgtag tcaataaaca
    20041 cttcgttgtc aggaataaaa ctaatcagag tgccgtcagc aagatcgcat gatttttctt
    20101 tttgctgttt ttttaattca cctctttcaa attctgcaat agtcgtttta ccttctctca
    20161 cactctgcac tttaaaataa gttgaaagtg cattcactgc tttagtacct acaccattga
    20221 gtccaacaga tttctgaaat gcctgtgagt catattttcc accggtattt attttggaaa
    20281 cacagtccac aactttacct aatggtatgc cacgaccgta atcacgaaca gtaacacttc
    20341 cgttttctac tgtaacatca atctgctttc catatcccat cacatattca tcaatggaat
    20401 tatctataac ctctttcaat aaaacataaa taccatcatc gcgcgaagac ccatcgccta
    20461 atttgccaat atacattccg ggcctgaggc ggatatgctc tttccagtct aaactttgaa
    20521 tgctgtcttc tgtgtaatta tgtgcaactt tttttgacat gcttcgatta ttttcttagt
    20581 tctttaaaat taaataaatt ggttgcaatg caatacattg accgattgct gaaattatac
    20641 ttttaataca gaccgttttg ctttaacaaa attcctttta aacaagtatc tgttaaaaaa
    20701 ttcagggggc aaagataagt ggtgtggatg cttcattttt catttcttca attgttgtta
    20761 aaaagtatta atactgaatt ttaggcggtt atcattttta ataattgttt gtatgcacag
    20821 gctttttttg ttaaagaact gtttatgttg tcgcattttg accttaggat caatcttttc
    20881 agtatattat ccaaaggaca tgaaagtttt atggtagaaa agatttttat aactcaatat
    20941 aataaacaag cagacgtttt gttttaattt gtaatttaga gcaatataaa aaatggaaaa
    21001 gcaacgcata ctttgggctg atgatgaaat agatttgtta aagccacaca ttttatttct
    21061 taatgataaa ggttatgaaa cggttacagc aaacagcgga aacgatgcat tagagatttt
    21121 caggaaagag cccttcgata tcgttttctt agatgagaat atgcccggac tcagtggcat
    21181 tgaaacgatg atgcgcatta agagcatcaa tagtaatatt cctgttgttc taattaccaa
    21241 gagtgaagaa gagtctatta tggaagatgc cattggttcg cagattgcag attatctgat
    21301 aaaacctgta aacccgaatc agattttatt gtcactgaaa aagaatcttg acaacaagcg
    21361 attggtcagt gaaaaaacaa acacacaata tcagcaggag ttcagacaga taggtatgat
    21421 gttaggcgac agacttgggc atgcagagtg ggctgagtta tataaaaaac ttgtttactg
    21481 ggaattgcaa ctcgataaag tccgagatga aagcatgttt gaaattctga tacaacaaaa
    21541 agaggaagcc aacaggttgt tttcaaaatt tgttgagagc aactatctca actggataag
    21601 aaatccggaa aacaatgcac ctctcatgtc gcagcatgtg cttaaacgaa aagtaattcc
    21661 tgttgcagaa acatcggatg tgcctgtttt ttttattctg attgataacc tgcgctttga
    21721 tcagtggaaa gttattgagc ctatcatcag tgaacttttc agagtgacag aagaagatac
    21781 ttatttcagt attttgccta ctgcaacaca gtatgcacgc aacagtctgt ttgcaggact
    21841 tatgcctgcc gacattgaaa aaatgcatcc aaaatggtgg gtcaatgatg atgatgaagg
    21901 cggtaaaaat ttacatgagg aagatttgct gaaagatcag atcagccgtt taagaaaaaa
    21961 tataaaatca tcctatacaa aaatcaccaa ccttgatgca gggaaggagc tggttgacaa
    22021 tattcccaat ttgtttggca acaaactcaa tgttattgtt tataattttg tagatatgct
    22081 ttcgcatgcg cgtacagaca tgcaggttat tcgcgaactt gcagatgatg aacctgcata
    22141 tcgttccatc acactttcct ggattgaaca ttcgccattg cttgaggcat tgaaacgcat
    22201 tgcacaaaaa aatgtaaaac tcattatcac aactgatcat ggtaccatca gagttaaaga
    22261 accatcaaaa ataattggtg acagaaacac caataccaac ttgcgctata agcaggggaa
    22321 aaatctgaat tatgaaaagc gcgatgtgtt tgaaataaaa aatcctgctg atgcactgtt
    22381 gccacgtctg aatgtgagta cagcttttgt ttttgctaaa caagataagt tttttgcata
    22441 tcccaacaac ttcaattatt acgtaaatca ttatcgaaat acatttcagc atggaggtat
    22501 ttcacttgaa gaaatgattg tgccatttgt ttcattggtt agcaaataaa agtaatgagt
    22561 gagcatgttt ttttttgtaa ctcaccggat gagttaaaca gtgttgcaaa acaaattctt
    22621 gccgtgattg gagaaagtaa aatagtattg tttaaaggtg agatgggggc aggcaagaca
    22681 acacttatca aagcgctgtg taagcaactt aatgtggctg acaatgtcag cagtcccact
    22741 ttctccattg tcaacgaata tcatgctgcc aatgaagtga tttatcattt cgacttttac
    22801 agaatcaata acctgcgaga agtttatgat atgggttatg aagaatattt tttcggtggg
    22861 caccgttgtt ttattgaatg gcctgaaaag atagaaggaa ttataaattt tgattatacg
    22921 cttgtgcaag tggatgcgaa agacgaaatt cgcaccataa ttgttacgat atgacaactg
    22981 cgtcaaagag tggtggaatg ttttcttcag aatcggtttt gcttccacgt gaaagcatgc
    23041 tcgaagtaca gaagacaaaa agagaagtgc tgattggtat tccaaaagaa atttcatttc
    23101 aggagcatcg tattgcatta acacccgatt cggtggccgt gctaacaagt aatggacatc
    23161 agataatggt tgaaaccggt gccggtgact ctgcaaattt tactgataca gattatagcg
    23221 aagcaggtgc aagaattgtt tatagcgcaa aagaagttta tactgcagat attatcataa
    23281 aaatcgcagc acctaccaat gatgagctag acttaatgca tgataaacag actttatttt
    23341 ctgtattgca ggttaatatg cagagtaaag atttctttct caaaatggcc aagaagaaaa
    23401 ttacagcaat cggttatgag tatatgaaag acgactcatc actcatgccg gtaatacaag
    23461 ccatgagtga aattgtgggt cgtacttcca tactgattgc atcagaatta ttgagtaaca
    23521 ctacaggagg aaagggagag ttgcttggag gcattgcagg tgtaccccct tcagaagttg
    23581 ttatacttgg tgccggcacg gtaggtgaaa atgctgcacg cacagctatg ggactcggtg
    23641 ctgatgtcaa aatttttgat gacaatatgt acaaactcag gcgtgtaatc agaaatcttg
    23701 gtgaacatgt ttttaccagt acattaacac agtccaccat gcttaaagcg ctgcgtactt
    23761 gtgatgttgc cattggtgct ttacatgcac gaacgggacg tacaccaatt gtagttacag
    23821 aagagatggt cagcgagatg aaatttggtt ctgtaattat tgatgtcagc attgaccagg
    23881 gcggatgttt cgaaacatct aaaccaacca atcacactca gcccacattc agagaatatg
    23941 gtgtggtgca ttattgtgtt cctaatattg caagccgtgt agctaaaaca gcttcgcatg
    24001 ctttgagcaa tatttttacg cccatactgt tgaatgttgg tgaacatggc ggattagaag
    24061 gattgttgtg gaaagatgct tcttttcgtt ccggagttta tctgttcagg ggtacggtaa
    24121 caaataaata tgtcagcgat ttgtatcgtt tgccttttcg tgatttgaat ttgctcatgg
    24181 caagttttac attttagttt cagataataa aaaacccgca ctatggcggg taattttttt
    24241 tgagtaaaca aacgtttagg gtatttcacg atagtatgtt actccgttga tggttattgc
    24301 tgcaatagca tcacaagtgc cgtcaccgta gtcaatgtat cgaatagcct gttcacccgg
    24361 agttagttcc actttgcctt tagtgataaa gcgacattgt aaagaaactg tcaaaggctc
    24421 ggtaatattg gcgctgaaag acgttccgtt aaatgatttt ccgctgccgc tgccggtaac
    24481 agcgtatata tcatccttta caccattaag actatcagta ttgaaagtgg tgttttctcc
    24541 ggcaatccaa gtgcgggtac gctgactttg ccatgtcatg gttttaccat tgggattact
    24601 gatgctgata tctgctgtga tggcaaaaac agattgattt tgattgtttt ttccggtatt
    24661 ggttattttt cttgttccct cagttttgta atcattcaca aaataatttt caaagcttat
    24721 agtatgctca tgatttgcat cgcgataatt cccttcatat tgaacgagaa tttttccacg
    24781 tcttctttta ccatctatgc aggtaacatc agttgcacca aagtcaagag tcattttttt
    24841 gatggttgca gttacatttt caatcgtcac tgctgcgcat ccgataacag taaccgtagc
    24901 tggagcacta tttctgtatg tcatcagctg tccgtaatcc ccagcctgat cagccatttt
    24961 agtgacttcc tccatggtgt tttctgctaa agacatatcg gttgccattg agtaatcata
    25021 gtcagcatca cttttatctt ttttgcaaga tgaaatactt attacgattg caagtgcaac
    25081 tattccgtaa ataaatgatt tgttcatttt ggataatttt ttagaagcct atacgtttat
    25141 tccggtaaat agtttcaatg attttttatt cagatgattt tagggtgatt attcatcagt
    25201 tatcagaagt tttatcactg aaaaacaggt tagttatcct caattttctg ttcataaaaa
    25261 gatgaataat caggtttaaa aacataagta ccctaagcac atcaataatt ttgcatttta
    25321 tctcaaatca gcaattaaac atggtaagaa tggtcaggcg aagtatctat ttaatgttat
    25381 tgttttttcc actgatgtcg ttggcacaac aaaccggcat ttacaccgat gtggaatggc
    25441 gttataaatc cgcattggat ttgctgcgca aagaaaaatt cagtgcagcg cgcgaggagt
    25501 ttacaaaagc attggaagaa ccaatttcag aagcagcacg tcaggatgca atgtattata
    25561 ttggtttatg tgcagcagag ttgtaccatc ctgatgcaga atttttactg acagatttta
    25621 tcagtaagta tccacagagt attaatgttt cattggcaca atttcaactt ggacgcattt
    25681 attacaagca acggaagtat cgcaatgcca tcactcattt tgaagcagta gatgttgcct
    25741 acctgaagaa tgacgaagta atagaatatt attttaagaa ggggtatagc tatttcacac
    25801 gaaaggacta tgataaggca gggaaaagtt tatcgcagat catcaatgtt gaatcaaaat
    25861 atcagacagc ggcacaatac tacaatgcac atgttgaata ttcaagaaat aacaataagg
    25921 cagcattgga acaattcatg aaactaaaaa attcagaatc attcggccct ttagtgccat
    25981 tgtatatcac acaaatttat tttgatcaac agaagtatga tgaagcactt gattatgcaa
    26041 taccggtatt gaaagtagag aatacacaaa ataagacaga gattatcagg attgttgctg
    26101 agtcatttta tcgtaaaggc aactatgcaa aagctatcag ttattttgaa gactactcta
    26161 aaaatactcc acagacaaca agacaggaga aatatcaggt tggtttttgc tactacatgc
    26221 ttaatgatta tgcaaaggca attgactacc tcaagcaggt gcttaacccc aaggacaaac
    26281 tcgcacagaa tgcttatttt catcttgcag attgttttct gaaaactgac gataagcaag
    26341 gtgcattaac ggcttttgag tctgcatcga agatgagtta tgatccgcag atacaggaag
    26401 aggccatgtt taactacgca aaactttctg ttgaccttaa catgcagtca tcaacaatca
    26461 gagtgatgaa tgattttatg actaaatatc caaagtccga acataaggat gagataacgg
    26521 agttgcttgc aacgatctat atgcagacaa gaaactataa agaagcatta gctgcattag
    26581 ataaaatcaa aaacagaact gcaacaggaa acgctgccta ccagaaagtg gcatattacc
    26641 gtgctgttga attattcaac gaccgcgact atgaacagtc aatcaggttg cttaataaag
    26701 ctattactac caacatagat ccactcattc aggcacaggc tatttattgg aagtcggaag
    26761 cattgtacaa tcagggtaaa tatgatgatg ctataaaaga gtaccgtatt tttattttca
    26821 cacccgctgc attgaagctc agcttttata atcgctcaaa ttacgacatg ggctattgtt
    26881 attacaagaa aggaaattac aaagaagcag caacttggtt tcgcaagtat gtagcatcaa
    26941 gaggtgagac agacaacaat cgctacaatg atgcattggt acgtataggt gatgcttatt
    27001 ttatgcaacg tgattttgcc aatgccgaaa atttttattc acaggcagta ggttcaaaag
    27061 cttcttcttc tgattattca ctttttcaac gtggaatcat tcttggtata aatggtaata
    27121 acagagcaaa agctgatgct atgcagcagt tgattacaac atatccaaaa tcaaattatg
    27181 ccgatgatgc ttattttgaa agagccaaag cgttgatggt actcgatcag gatgatgaag
    27241 cagagttggc ttacaataaa ctcatcagtt catatccgac cagtccgtat attaaaaaat
    27301 cattgctcaa taaaggtctc atttatttta ataataaaaa tgatgaaaaa gcaatcgaaa
    27361 catttaagca ggttgtaaca aagtatccat caacaccgga atcggtcgag gcattaaatc
    27421 aaattaaaaa tatttatgta gctcagggaa atcccaatgc ctatttcgat tatgtaaaga
    27481 aaattcctaa taatgcagtc agcagtggcg cacaggattc tattacctat gaagcagcag
    27541 agcaacgcta tatgaaaggt gattataaaa atgcttctaa tgattttgca caatatatgt
    27601 cgcgattccc taatggttcc tacatcttga atgcaacttt ttataaagca gaatgtgaat
    27661 acatggcaaa agactacacc aatgcactta aaggttatga aatagtaaat aatgccacaa
    27721 agaatctatt tactgaacgc tcactcatca gatcatcggc tatttattac acacagaaaa
    27781 attatgatca ggcgcttact gcctatcagc gattggaatc taatgccgac tatgctgaca
    27841 atttattgat agcacaaaca ggctctatgc ggagttacga taaattgaag aattgcagtc
    27901 aggcaatagt atatgccgat aagctactgt ccaacgataa gacagatcca acagttattt
    27961 cagaagcaag attgataaaa gcacgttgct caatggaagc aaaggattat gctgcggcag
    28021 ccaaagaatt taatttgatt gctaagggta gtaacagtga agttgtggca gaggcaaaat
    28081 atacgcttgc gtttattcaa tacaaatggg gcaattacaa agagtcgcag aaaaaatgtt
    28141 ttgaagtgat taaacaagtt ccttcttatg attattggat tggcaaagcg tttattctct
    28201 taggcgataa ttatgttgct ctgaaagata attttcaggc taaacagacc tatcaaagta
    28261 ttattgataa ctatgaaaag aacccttcag accccgaaga cctgaagcaa attgcaacag
    28321 aaaagttaaa tgtgctgata acaacagagg gtgttcctca accacaacaa cctaaggagg
    28381 aggaaaacaa tgaaaactaa ttcaacaatc gaatcaggaa tgaatacagc aatcatgtta
    28441 aagaaaacaa tattctttgg tgcattaggc ctagcttttt tacagtcgaa ggcacaaaca
    28501 cgcgacacca ttaaaaatat tggtgatgag aatattattg ttgttaaaga ctatcaaccg
    28561 acattgtctg atgcagtaaa gattagtgat atgccgggtg ccgatacatc atcaatcaaa
    28621 gaagtgaaat ttaaatatac catagaaggt aagcgtgctg atttaccttt taatacttca
    28681 ccaataaaac ctgttaaaat taaagatgat gaaataaaac tgctgcataa aggctatgtg
    28741 cgtggaggat atggaacaca caacactccc tatgctgaat tgttttttaa tgcactacgg
    28801 tcaaaggaat ttgatgcagg aatacatctg aaacatcatt catcaacagg aaaaattaaa
    28861 aactacggca agactgacaa tagtgagaat gcgcttgagt tattcggtaa aaaatttact
    28921 gattacggta cgctcagaac aaaattaaat ttcgacagag atgtgtatcg tttttttggt
    28981 tatgatggaa agtcaactgt atatagcaaa gctgaaacac gtcagttgtt tagtggtatt
    29041 gcaggtgaag taggatttga taatttttca aagcaagagt caacatataa gtttgatgca
    29101 ggtttgtcgt tttatggtta tagcggaaaa cgcgatacaa gtaagacaac agaaaatagt
    29161 gtaatcttag ccgggaaatt acagcgtaaa attgctgaca gagatgcatt tgcagacatt
    29221 aaaattgatt attcccgcac aaaacttcct tttgatacat tagttaaaaa cactattgtg
    29281 ggtgtttatc cacgctacac acttgagttt ccggagtttc gtttaattgg tggtgtcaat
    29341 acagaatggg aggcaaacag tgcagctaag tttcatttgt atccacacgt agaagcccga
    29401 tataaactgg ccagtgatgt tttgatgata catgcgcagg tttccggagc tatgcaaaag
    29461 aacacctata aggcaatgac agcggaaaat ccatttacag gtgattttgt tatgccagga
    29521 aatacaaaca aaaagtttga tgtttcagga ggactgaata taaagttaga taaagaaatt
    29581 ctttttagtg caggagcatc tttcaagaga ttaaaagatg cagtttactt tgaaaacgat
    29641 tctacaggtg ctttaaacta tacaaccttc tctactgttt acagcgatgc tgatgtgatt
    29701 actttaaatg gtgaattggt atttgagcgc aatgaaaaat tcaatgcaca tattggcgca
    29761 acttacacca ataataagcc cgatggactt gcaaaggcat ggtttgttcc tgctgtagaa
    29821 attaacttag gcggatatgt tttactgaga gagaaaatcc tctttaaaac cgatatgttt
    29881 tatcgtggcg aacgatttgc agctgcttat aatggtgact cattccagcg cttaaagccc
    29941 tattttgatg ctaatctttc tgttgaatac cgctactcaa aaatggtgtc tgtatttctt
    30001 aatatcaaca acattggtgt ggtacagtat tttaaatggt ataactaccc atcttacagg
    30061 ttgcaattgc tggctggcgc ttctttgtcg ttttaaattg aagctatcta attaaacggc
    30121 tgcccgattc gggcagctgt gttgtttatg ggaaatccat agagcatgcc agataacatc
    30181 aagcttatgc aatacattaa attgtaaaaa ttgatgtatt acaaataaaa cgatatttgc
    30241 aacaatgcat aagtaattat gaattcaggc aattgaaaaa tcttttcact ataatatttg
    30301 tagtctgttc tgttttgagt agtatggctc aaagaagtcg taacacaaat cgtacacatg
    30361 gacttgatgt tactatcagc aggttagttc ctaatttttc taaggtgccg gatcagaatt
    30421 ttttatttca tgaaagtttg acttattcca ttgttgccgg acaacgtatc cgtgcattag
    30481 caggaatcaa ctttttggca cctgcaaaag gaggtgtaac acggtatggt tttcatattg
    30541 gctttcttgc cgggtcaaaa aatccaactt cagggctcat ttacgggccg gttctttttc
    30601 atatagtgaa taaaactgct tatgacaatg gtgcattgcg aaactatcat aataccggtc
    30661 ttgccttggc agtaggaatg tattatgttc ctgtaagcca gtttgttttg tcaacaaaaa
    30721 ttgctcctgc aatctatcat tatagtgtga gaaaaaagaa tgatatcaag gtgattgata
    30781 ataattcaga ctcccgctcc gagacttttt atatgggttt ttataatctt gtgagcctga
    30841 gtgtaggata ccgtttttag gagaaagttg atggtttaaa aaatcatctc aatacatact
    30901 ttccttagca aataaaccat tacttttgtg ttcagtaatt tttattaaaa tgagtaagac
    30961 aaacgaggta ttagaccagg taatcataaa atttgccggt gattccggtg atggaatgca
    31021 gttgaccgga acgcaattta caaacacagc cgcattgttt ggaaatgatt taagtacatt
    31081 tcctgatttt ccggcagaaa ttcgtgctcc gcaaggaact gttgcaggtg tttcaggcta
    31141 tcagttgcat ttcggaagtg taaacatttt tactcctgga gataaatgtg atgtacttgt
    31201 ggcaatgaat gcagcagcat taaaagtaaa tcttaaaaat cttaaagact caggaacaat
    31261 cattgccaac agcgatggat ttgatccgaa gaatttgcga ttagcaaaat atcctgatgg
    31321 tcaaaatcct attgaagatg gttcattagg taaatataat ctggtagtga ttgatgtcac
    31381 aaaaatgaca cgtgctgcat tggttgattc aggcttaggt accaaagaaa tagaccgtgc
    31441 caaaaacatg tttgtgcttg ggtttctata ttggatgtac aaccgctcgc tcgacaacag
    31501 tataaaattt ctggaagaaa aatttggaaa aaaacaagct atacttgatg caaacattaa
    31561 ggtgttgaaa gccggttatc actatggtga tacaacagaa actttccgta cacgatttac
    31621 tgttcagcct gcaaaaatga agcccggtgt ttatcgtaac atcatgggaa atcaggcaac
    31681 ggcaataggt ttaattgctg ctgcacaaaa aggaggaata gatttgtttt ttggaacgta
    31741 tccaattact cctgcatcag acattttgca tgaactctct aagtataaaa attttggaat
    31801 aaaaacattt caggccgaag atgaaatcgc agccgtttgt gcaacaatag gtgcatcatt
    31861 tggtggttca ttgggagtta catcgtcatc aggacccggt attgcactaa aaggtgaagc
    31921 tatgggttta gctacaatgc ttgaaattcc actggtgatt attaatgttc agcgtggagg
    31981 tccttcaaca ggattgccaa ccaagacaga acaaagtgat ttgatgcagg cactttatgg
    32041 cagaaatgga gaagcaccta tgcctgttgt ttcatcgtca tctcctgcag attgctttaa
    32101 tacaatttat gaagcctgcc gtatagctat tgaatatatg acacctgtta tttttctttc
    32161 tgatggttat attgctaatg gtgctgagcc atggcaattt ccttctgccg atcagttaag
    32221 agaattcaaa gtgaaatatg caactgaaac aaatggagag cacttccttc cttataaacg
    32281 tgatgaaaaa ttatcacgcc catgggcaat accgggcaca aaaggacttg aacatcgcat
    32341 aggtggtctt gaaaagcagc atgaaacagg taatataagc tacgacaacg acaaccatca
    32401 gtttatggtt aaaatgcgcg aagctaaagt ggaaaaggta gctgacagca ttccattgca
    32461 gcagttagat agcggacctg aaaaaggcaa attacttatt gtaggttggg gtagtaccta
    32521 tggtgctatt aaaacagctt gtcagatggc acgtagcgaa ggatatgatg ttgctcatgc
    32581 acatcttact tatctgaaac cattcccaaa aaatttcggt gaaatgttgt ttaaatatga
    32641 caaagtgttg atgcctgaaa tgaacatggg tcaactttca agagttgtta gggataagtt
    32701 tatgatacct gcaatcagcc tcaataaagt aatgggaata ccgttttccg caactgaaat
    32761 caaagagaag attgttgaat taatgaaata gtaaaaaagt aaaatttaaa tcagttttta
    32821 atagatagta tttttatgga agcaactgca acaacaccca caccgttata tacttcaaaa
    32881 gatttagtaa ccgatcagga tatacgttgg tgtccgggat gtggcgatta ttcaatatta
    32941 aagcaggtac agaccgttat tcctgagttg ggtctgccac gtgaacagat tgtttttatt
    33001 tcaggcattg gatgctcgtc acgttttcca tattatatgg agacctatgg catgcactct
    33061 attcatggaa gagcaactgc tatagcgagt ggactaaaag ctgcacgcaa agatcttagt
    33121 gtttggattg taacaggtga tggcgactca atgtctatcg gtggaaatca cttcatacat
    33181 ctgttgcgca gaaattttaa tgtaaatgtt ctgttgttta acaatcagat atatggactt
    33241 acaaaaggac agtattcacc aacatcagaa tcaggtaaag tgactaagtc aacacctttt
    33301 ggctctattg atcatccttt taatcctgct gcattggcaa tgggtgccga tggaactttt
    33361 gtagcacgaa gcatggatcg tgatcctgta catttgaaag aaatgctccg tcaggcagca
    33421 atgcataagg gtacttcttt cctcgaaatt tatcagaact gcaacgtatt taatgatggt
    33481 gcatttgaag tgtttaccga aaaagcatct aaaaaacaag aagcactttt tgttgagcat
    33541 ggaaaacctt tggtgtttgg tcagaatggg gaattaggaa ttaaacttga tggatttaaa
    33601 cctgtcatcg ttaatcatgc tgaaaaggga atcagtacta acgacctttg gattcatgat
    33661 atgttcgaca gagtgaagtc cggaatttta gcacgtttct ttgataatcc tgccaacgaa
    33721 ggccacttcc cccgcccatt tggtgtgttc tataaagaag aaagaagttg ctatgaagat
    33781 gatttgatac aacaagttga agcagttatt tcattaaaag gaaaaggtga tttgaataaa
    33841 cttttaagag gaaatgaagt ttgggagatt caataaatcc tgaataatat tttattacca
    33901 ttattttaaa ctgttattgt tttctttcac gataacagtt gtagtggcta ttcttttacc
    33961 ggtttttaag caacaatagt tttttgataa cttaaatttg attctatgat tgtatttata
    34021 ggatatcttg catcagcatt tttagctatt tcattgttga tgagcaatgc ttttaaattc
    34081 agatggtata atctggcagg gcaaattgca tttattgttt atggatattt catcaattca
    34141 atgcctgttg ttattgccaa cgcaatttta atggtcatta atatctatca gataatacaa
    34201 ctttacaaaa gtaaagaagc atttgatgtt attcccgtta aagaaagtga gccactggca
    34261 gaacgattta ttgagcgaaa caaggaggag atattggaat attttcctca gtttgaaatc
    34321 aataggactt ccggctttgc aattattgta tttcgagata tgacagcagc caatattttt
    34381 attgctgatg tttcagaaga tggtaatgca actgtaaggt tgaattatac cattccaaaa
    34441 tacagagatt ataaagttgg aaaattctta tttgaattgg agaaagattt cttaatcaga
    34501 aacggattaa aaaagataac ctataagaac ctgacacatg ccggacatat tcgctttata
    34561 aactcattag gtttcagcaa aggtgatgat tcaggaaact attttaaaat actgaattaa
    34621 gactgattac ttacttagga taagtttctt tgttgctgaa tgtacaccat cactaacctg
    34681 gagtaaatat gcgccagctg ttaaagacgg tgtctttata gcaatgaatt tttcttttaa
    34741 ctcagaagta gtatggtaaa ctatttttcc ggtaacatca gatagttgaa ttattatttt
    34801 gttttttggt tgttcaacaa aatgaatatt cagttcagaa gtattgaata taataccgga
    34861 aataaatcca tcattcttta cttcgttaat cccagttgcg gcactcattc tatcaaagta
    34921 aacccaactg ccgttaccac tgttttcaac ccatgctaca cccacatagt tggtagttcc
    34981 gctaaaagcc gaaattacag gtaaatgata ggaaggcgtg tcaacaatat taaaatcact
    35041 ataggcaaca ggtgtgctga atactgaagg attattaagc gaggcattgg taaacatcat
    35101 cggaaacgga gttggtggta gtgtagcatt ggagttatag taagttaaat aacaacccca
    35161 ggcaccaaaa aattcataat gacaggcatc aaaattataa ttactaaaac cattattgcc
    35221 tgcaactatt tgttcggccc cgtaattatc tccattatcg gtactgatac gacattttat
    35281 tacttgggaa gtgtctcctt ctgtaaagaa aaaccaaacg gtattataat tgtaaaccgt
    35341 cttgaactgt ttctttttta cagcggtatt ggtaacaata tcaataaaag cagtagcacc
    35401 tattgtcccc ggaacacttt catcataagt ggctgctctt atttttgaag tagcaggatc
    35461 ggcatttaca ggaccataat aatttaatat caaacgtgta cctgacatat atactttagg
    35521 tgtttgccct gacgatgtta cagttgcagt gctgccgccc caggttaaac caccatctaa
    35581 agttgtatag cgttttatgg taaagtttat gggttcatta acaaataaat aaataacatt
    35641 gcctgccggt gaacctgcag catcaaaatc tgcaacagaa gacgatgtaa atgaacctat
    35701 aattcctgtt tcaacattta ataaatatac accagctgtt gtgctgtaca ggcaataaac
    35761 actgtcactt cctgttcgca ccatttttgt ttttgtaatg gcagttgcca taggccctgt
    35821 tgcattactt acaggaagcc atgtgtttcc atcgttggtt gattttaata attggatgga
    35881 gtacatgccc gcactgctgt cgggtacagc aaggtacaat gtgccattac tttttgccgc
    35941 catactatgt tgtgcaatcg gtttgttgtt ataaactaaa atatcgttcc accatgcaaa
    36001 tacatttgat gtggaaataa ctaataagat tgaaagaaat ttataacgta ttttcatatt
    36061 tattttgttt ataacactaa agtagttttt ttctgtataa gaataatctt ttttaactaa
    36121 ttcttttctg catattcatt gttcagttag ttatcttcgc agcctaaatt tatatccatg
    36181 cgattattag tcactttatt aacagttgca atagttgttg ttgcatgtaa gaattcaaaa
    36241 acacccgtgc agactcatga tgagttggtg caacacattg acagcagtgt tcgtcccggt
    36301 gaagattttt ttatgtttgc taacggacga tggtttgcac aaaatccgat tcctgcaagc
    36361 gaacgcagta atggtatttt cagaactatt aacgatacga tcaacgcaac gattttgcag
    36421 atttgtcaaa cagcagccag cagtacaaat gacaaaggca gcctcaagca aaaaattggt
    36481 gattattatt caagtggtat ggatactatc ggaattgaca aagccggtat tgcacctcta
    36541 caagaatatt tttctgagat agatcagatg aaaactttta atgatgtgat gaaagtggca
    36601 gcacatatac attgtatttc tgcttcacca ctttttgctt tttatgttaa ctccgatgat
    36661 atgatcagct caaagaatgc tgttttctta gcacaaggcg gattgggttt acctgatcgg
    36721 gattattatt ttaatactga tgaaagaaca aagaaagtaa gagaggcata tcctgaacat
    36781 ttgaaaaaga tgtttgagtt gagtggcttc gatgaaaaga aagcaaaaga tgctgcagct
    36841 gacgtttacg ctgtagaatt tgccttggca aaagtcagtc gtaagatgga agacctgcgc
    36901 gatccattta agaattataa caagacatca gtaaaagaat tgcaggagaa aatgaataat
    36961 gtgccaatga atgaattctt ccatggactg ggaatgaatt tacctgacac cgttattgtt
    37021 gggcaaccgg agttttttac ttatctcaac atagcaacac gtcagtttgg gattgaagtt
    37081 tggaagaatt atttacgctg gcatttgtta cgtggacttg caccttactt aaatactgag
    37141 attgagaaac agaattttag tttttatgga acattgttaa atggtgtaaa ggaacaacgt
    37201 ccaagatgga aaagagtggt tgaagatact gatgggcagt tgggtgagtt ggtaggacag
    37261 atttatgtgg agcaatattt acctaaagga acaaaagaaa agttgatgga aattggcaat
    37321 gcagttaaag aagtttatcg caaacgcata gcaggacttg actggatgag tgatgtaaca
    37381 aaagcaaagg caattgcaaa actcgatgcc atgattatga aagttggcta tccggataag
    37441 tggaaggata tgagtgcatt ggaagttgac aaaacatctt atgcaggcaa tgtgattcgc
    37501 gccaatgcat ggcattttga ttatatgaaa aacaaatatg gtaaacctgt tgaccgtact
    37561 gagtggagta tgacaccaca gacttataat gcctactaca acccttccaa taatgaaata
    37621 gttgttcctg catgcaacat taccgttccg ggatatgaga aaactttagc tgacgatgca
    37681 attctttaca gcattattgg tggaagtaca tttggtcatg agttcaccca tggttttgac
    37741 gatcagggaa gcaaatacga tcagtttggt aatctgaata attggtggac atcagaagac
    37801 agcatccgct tttatgcaaa gaccggtgcc gttgtgaatc agtacagtca ttatcttgtt
    37861 gttgacagtt tgtatattaa tggcgatatg acacagggtg aaaacattgc cgacatcggt
    37921 ggaattgcaa tgggtcttga agcatttaag caaaccaaac agtttaaaga aaatcaagtg
    37981 atagcagggc ttaatcctat gcagcgtttt tttctaggtt atgcttatgc atggatggta
    38041 aacatgcgcc cggaagcatt agcaacaaga gtgctcactg atgtgcactc acctgctaag
    38101 ttcagagtta ttggaccctt ggtgaatatg ccggaatttt attctacctt tggtataaag
    38161 acaggagata aaatttttct tccggagagt gaacgtgtac atatctggta aattattagt
    38221 tttgaaaaaa cttgtgtaat gagagaagag tcaataaaat attattcgcc aataataggt
    38281 cgtgattttg aaatgctcat tttcggtgag agcggtattc cggtaattgc atttccaaca
    38341 agtatgggca ggcactatca aaaccgcgat tttaagttga tagaaagtgt tgccccattt
    38401 gtggaaaatg gatttataaa aatttattgc cctgacagta ttgatgagca gagttggtac
    38461 aatacacaca ttcatcctgc cgaccgggta cgcaatcaca tactatacga tcagatgatt
    38521 ttagaagaag ttgtaaggcg tgcacaaaaa gaaacatcac acagtaaagt aataactgcc
    38581 ggatgcagtt ttggtggtta tcaggctata aactttgcct ttcgtcatcc tgaagttatt
    38641 gcgcatactt tcagtatggg tggtgctttt gatattaaga tgcatcttga tggttattat
    38701 gatgacaatg tttatttcaa taaccctccg gattatttac ccgatctgga taacgaacat
    38761 ttgtttcaga tgggaattgt attgggcgta ggcgaacatg atttttgtat agctcaaaac
    38821 aaacgtcttt cagaaattat gactaaaaaa ggaatggcac attggcttga tgtacgcccc
    38881 ggtgccaacc acgactggcc tgtgtggcgc gaaatgtttc cggcatacat tgaagcatta
    38941 cagaccgcta aggtttagga ttgtttttaa tgttgagcga aagctgacag gggtgaggag
    39001 ttaatacggt gaagcctaat gataaaaagt acctgagttt tttggtggaa actcaagttc
    39061 tgagtgagga tgaagcgttt atgcttcaaa ctaaaacctg aggttaggca tggtcttttg
    39121 tctgtaactt acgactgaaa atctcgaaga tagagatgcc catcattcta tcaaaaccaa
    39181 aatttatttt aattcatcat tctgaatgtt acattctgaa tggatttcct ttaccgactc
    39241 aaacctgccg tttaccgatt tgtttttctg tttacttagt ttatggctgc taataaccta
    39301 ttgtgtctat gaactaccta tgagggcaac atatctttgt cccataattt ttaaagatgt
    39361 aatataatgg aaaatcaaaa agaaaaatct gaaattgaag acgtaagaga agacgtatgg
    39421 cgcagatttg aaaaagataa tcagcgtggt aaaatatttg gcggcttaat aatcatttgt
    39481 gttggtgcat tattgtttgc cagagaagcc ggtgtatatt ttccgtcatg gattttttca
    39541 tggccaatga ttttgattgt tataggtata tttctgggag taaaacatgc attccgaaat
    39601 atggcatgga tggtcctggt gcttattggt ggcttgttct tgatacgaaa cagtttcccg
    39661 atttttgaat atagaaatta tatatggcct attgttatta ttctattggg attgtttatc
    39721 attattaaac cttctaatcg ttttcgcaga gcatgcagac gaggcaggtt tggtacaaga
    39781 catcaacaaa atcaatttgg aaactattcc aatgcaccgg catcaggaga agatttttta
    39841 gaagcagttg cagttttcgg aagcataaaa aaaaatatca taagtaagta ctttaaaggt
    39901 ggcgaagtgt cagctgtgtt tggtggtgca gaaattaatt ttatgcaagc cgacatccaa
    39961 ggtagggtag aattggaggt gaatcaggtt tttggaggaa caaaacttat tgtccctgca
    40021 cattgggaaa taaaatcgga aattgctgca gtgttaggaa gtgttgaaga taaacgtcca
    40081 ttacggggta caacaaccga tgaaaaaaag gtgctgatat tgcgaggtac tacagtattt
    40141 ggaggaatag acattaatag ttattaattt tttaaattca ttttttttct aaactaaatt
    40201 aaaatgtatt ttgtagctaa agtaatttat cagataataa tcaagggaag caaaaataat
    40261 caattcgatg agcaactttt aatcattaag gcacaaaatg tagccgaagc ttatttaaaa
    40321 gcacgtgcaa taggagcaga aaagcagcat gagtttatta atgatagaaa gaacaatgtt
    40381 aaatggcaaa tgattgatgt tgcagatatt atgcctgttg aatctcccga gcatggttct
    40441 gttgtatatt cgcaagttat agaagaagat tcagaatcgt ttatagatta tattaaacga
    40501 aaggcaatgg tacttcaaac gcaatcactt ttgtttgctt gatagagaat gattattgaa
    40561 aaaataaaaa attcaattga ccagattatt tggtggctat gttggtattt ttttgtcgca
    40621 gcagtagttc accaaaatgg atttgactgg tatctgtctt ttgcagacac tacagtaatg
    40681 ttgctattat tgttatttac ttcgtgtagt acctattttg cattcaataa atttcaaaaa
    40741 acagggaatc gcttttttgt gattttgtgg aatattgcat tatcactcat ttctgttttt
    40801 atattgagtt tggtattaaa aaatatttat attgataatt taaaatattt agcctttctt
    40861 gatgcatcaa ttatagttcg gtttacatgg ttttttataa tgaatctttt agtgactgtt
    40921 atctatggtt tgcaagcaaa tattttggag caaaaaaaat tagaagcaca taataatgat
    40981 aatatcaacc tgatgaaaga tgctgaactc tcaggattac gcttgcaact gcaacctcat
    41041 tttttgttca acagccttaa ttctatcaat gcattaatag gtacagaacc ggcaaaagca
    41101 agaagtatgg tattgcagct ttcagaattt ttgcgtggca ctttacaaag agacaataag
    41161 aaattgataa cccttcagga ggaagtaaat catctgaaaa tttatcttga aattgagaaa
    41221 gtacggtttg gtcatagatt gataactcaa attgaaatac cggagaactg tttgaatctg
    41281 ttggtgccac cacttatttt gcaacctatt gtagagaatg ccattaagtt tggactctat
    41341 gacaccactg gtcaagtgac aatttcattg caggtttcct gtaatgatag tcatttaaag
    41401 atagttgtta ctaacccttt tgatcctgat actgctgcat cacaaaaagg caccggcttt
    41461 ggtcttgacg cagtgcaaag gcgattgtat ctgctttatg cgcgaaatga tttacttaga
    41521 acagaaaagg caaataatat tttccttaca caaatagaca ttcctcaaat atgattaaag
    41581 ctattgtgat tgatgatgag cctcttgcaa gaagccttgt actggagttc cttaaagcat
    41641 ttccaaacat tgaagttgta caggaatgta atgatggctt tgaaggactg aaggctattc
    41701 aacagtttca gccgcaactt atttttctgg atatacagat gcctaaaatt aatggctttg
    41761 aaatgctgga gttgattgaa aacccacccg gtgtaatttt tacaactgca tttgacaact
    41821 atgcaataaa agcttttgaa gcgcatgcca ttgattacct tctgaagcca tttagccaag
    41881 agcgatttta caaatcagtt actaaatttc ttgaaacaaa tagtaatgcc gctgtaatgg
    41941 atttagtaaa tgcggaaagc aatattctgc ccgatgagaa gaatcgtatt gttgtaaaag
    42001 atggaaatcg gattacgatt attcctgttg atgatgtaat ttatatagaa gcgtatgatg
    42061 attatgtaaa agtacatata cagaatggct ttaagctgaa aaagaaaact atgtcgtact
    42121 atgaaaatac acttgataaa tcacgattcc tccgtgttca tagaagctat attgttaacc
    42181 tgagccttat taccagaata gaacagtatg aaaaaacatc tcacattgct ttacttaaaa
    42241 acggacagaa aatccctttg agcaaacaag gttatgcaag attgaaatct gttttaggta
    42301 tataggttaa aacgaaaatg cttttttatc tttattaaag tgaatagtca ttcttgtttt
    42361 acaggctgtt tctttttgtg ctttggcatc aacagggttt aatttttcca gaaagatatt
    42421 aatgctgtag gagtaatttt tcttgcttaa tttaaccgga agatgggtgt tgacttcaaa
    42481 cttgtaagct acagcatcag gtttaatata tgtgacttga tagtgattgt ttagttttag
    42541 tttaagcgtg ctttcttttg atgtgctcaa acggtttaca tttcctgatt catcagaaat
    42601 taccacaaca attgaatcat acatattgtc ttccgctgct atgcgggtct ttaatttcaa
    42661 ccattgcgcc tgacagttta cggtatagaa aattatcaca aatagcagac acgttttaat
    42721 cattctcgtg cagactttat ttgatgttta tacatctgca ccgtattttg cagtccataa
    42781 tataatgcat cactaatcaa agcatgaccg attgatactt caacaatctg cggtatattt
    42841 tttataagat aattcaggtt gttcagattt aaatcatgtc ctgcattaac acccattttt
    42901 aagaatgcag catgtgcagc agctttttga taatcagcta ttgcctgttg aggagatttt
    42961 tcgaatcctg atgcatagcg ttctgtatat agttctattc tgtctgcacc tgttgctgcc
    43021 gctgcatcta tttgttttag gtcagtatcc ataaataccg acaccctcat accattttgt
    43081 tttaatctat caattatttt tttcaaaaat gcttgctcac gaatagcatc ccaacccgca
    43141 ttggaagtaa gtaccattgg agaatcggga accagggtaa cctgatgtgg ctttacagac
    43201 tcaaccaatt tcaaaaaatc gtctgaagga tatccttcaa tattaaattc tgtagtaaca
    43261 cattctttta attcataaac atctctgaaa cggatatgtc tttcgtcagg acgtgggtga
    43321 acagttattc cttcaccacc aaaacgttca atatctaaag ctgcttttac cacatttgga
    43381 atagttccac ctcgggcatt ccgtaatgtt gcaattttat taatgttgac actaagttta
    43441 catgctgtca tatactctgt aaatgtgaga caaatgtact atgtttgccg gcagattcga
    43501 ttcgggaaga aatattcatg aacacaaagg cttatattaa agattttatt ccaccactaa
    43561 aaagttccga ctctgtagtt agagctttac aatggatgca ggcttttaat ttaaatcatc
    43621 tgcctgtggt tgatggtgta aagtacaaag gcatcgtaac cgagaacgat cttaaaaaga
    43681 ttgaagacaa agaaaagacg cttgcagcat gtgatttgga atatcagaat gtttacattt
    43741 ttgagacaca atatatttat gatgctttgg agtttgttac tgtaaaaggt ctgaatattg
    43801 ttcccgttat cagtaaagaa ggtaattatc gtgggcttct taccttagtt gatattatag
    43861 attgttttgc tcagaacagc tctgttaaaa cacccggagg tatcattatt ctgaatgtgg
    43921 acagaaagaa ttattctatg caggaaattg caagaataac agaatcgaac ggggctttaa
    43981 ttttaagttc atcagtaaat caacttccgg atagtgaaat ctttgaagta actttaaagg
    44041 ttgataaact tgatctgtcg cgcatacttg cagggtttta cagaatggac tataatgttg
    44101 ttgcatcata tcagaccagc gatttgccaa cagatttgga agaccgttat caggcattta
    44161 tgacttacct taatgtttaa aagagcatta ttaataaatc tgttttttgc attttgtttt
    44221 agcagttttg cttccgattc tttacaagta gatacaagta caatttatgt aagacagatt
    44281 gttgttactg gaaataaagt aacgcgttat tctgtcatcc aacgtgagtt ggcattcagt
    44341 cagggacaaa caatgaaagt tgctgagttg gatgaaaaaa tcacaaaaag cagagagaac
    44401 ctattaaaca tttctctttt caattttgtg aatatcaaca agcagctaat aggcaatgat
    44461 gtatatgtga ttattgatgt tgttgagcga tggtatattt ttcctgtacc tattttcgaa
    44521 gttgtggacc gtaatttttg ggaatggtgg cgcgataaaa atctgaagaa agcgaattat
    44581 ggtttttatt tgaattggga aaatttcaga ggtaggaggg agaatgtaaa gttgttactg
    44641 cgttatggct attcgcaaag gcagggattt atgtacagca ttcccagtat tgataaaagt
    44701 caaaataatg ggttggtgtt tactgcttta caaacaagaa tgcgcgagat tccctacaag
    44761 ttagaaaaca acaaattggt ttattttgaa aatggaagtg agtttttgag aaaggaatat
    44821 attgcaaacg taagattcat tcaccggcag ggttttaata agacctatac ttttaccgga
    44881 ggctatcagt acaacataat ctccgatact gttctttctc tcaacagaga ttattttgtt
    44941 agtggacata agtatgaaag ttatcctgta atttcttttg aatataaaga tgatgcccgc
    45001 gatatcaaaa attatccatt aagaggttat tttttaagtc tagaatttac aaaatatggc
    45061 tttggcatca gcgataacga cccatcaata acttatatta tagcacattt taaaaagttt
    45121 tggaaactga acaatacatg ggcagcagca ttttctttta aaggcaaatt gtccggaaat
    45181 aatgaagtac cttattataa tcagatagct ttgggctaca gaggcgattt agtcagaggt
    45241 tatgatcctt atgttatcaa tggtgaaaat tttactcttt ttaaaagtgg aattaaatat
    45301 gccatagttc aacctaagtc atttaatgtt cctgttggtt tgccacagag ttttacacgg
    45361 ataccttatg ccctttatgc caatttattt tttgatacag gctatgttcg cgacaagcgc
    45421 ttctatcaaa acaacccttt gtcaaacaaa tggctctata gctacggtgc aggtcttgac
    45481 tttgtaacct actatgattt ggtgcttcgt ggcgaatttg ccattaccgg aaatggtaaa
    45541 tccggaattt ttctgcacct tacagcgcca atttaataac tttgctcgat gacaattgcc
    45601 gtatatgcca gaaatgtaaa cgaaaaagtg cttgcagcac tcgatgttgt atggacacaa
    45661 ttacgtcaga atgatgttga tgttttattg tatggaacat tgtttcgtca tttgaaaaag
    45721 ttaggtaatg aaatgcaaaa cactcatgtt ttcagcactt catctgagat agcaggtaaa
    45781 gccgattttc tattcagcat tggcggtgac ggcacaattc tcgacacaat taaattggtt
    45841 aaaaattcag gaattcctgt attaggcatc aacaccggca gacttggttt tttgtctatc
    45901 gttttgcccg aggcaattac cgaagccata aacagtattc tcaaagggca ttattctctc
    45961 gataaaagag ccatgctcag gctggaaact gataaagaga tttttggtga tgtgccctat
    46021 gcgttaaatg aacttgcaat tcataagaag gatagctcat ccatgataat aattcatgcc
    46081 ttcttaaatg gtgaatatct gaattcctat tgggctgatg gacttctgat tgcaacaccc
    46141 accggttcaa caggatattc tttaagttgt ggagggcctg taattgcacc ggcttcagga
    46201 aattttgtga ttacacccat tgccccacat aaccttaatg tgcgtcctat cgtagtatcg
    46261 gataaaaatg tagtttcttt ggaagtggaa ggtcgaaacc catattttat ggcatcattg
    46321 gactccagat cagtaaccat agattcatcc gtacaactgg ctgtcaggcg ggaggacttt
    46381 agtttcaatc tggtaaggtt cgataacgat aatttccttc aaaccctaag aaataagcta
    46441 aattggggcc ttgatgcccg taattggtaa gaatatatta taattgtacc tgcaaatatt
    46501 tgaaatgttt atttttgtca ataaccaccc cggcttatga acgataaata caagaaatta
    46561 ctgcttgttg gcttaatttt aattcccttt ttaggtagtg cacaggttgc taaaaaacgc
    46621 tggaaggcat accgtattga gtatattgca ggactcggag taaccaattt tttaggtgaa
    46681 cttggaggtg ctaacagagt aggaactcat gctttccgcg atttggaatt ttcgatgacg
    46741 cgccctgctg caatgattgg ctatcgctat aaactttcac caacttttgc atggtgctct
    46801 aaattctcct atggaattgt ttccggtgat gatgccttaa caaaagagca tttcagaaat
    46861 aaccgtaacc tcagttttaa atcaaacatt tttgaactca gcaccaatat tgaaatttca
    46921 tttttgcaag aacaagttgg tcaccgctac aaactccgtg gtgtcagagg tatgcggaat
    46981 gtcgaaatat ctgcctatgg atttttaggt attggcgttt ttcactttaa tccaaaagct
    47041 aaacttgacg gacaatggta tcaacttcag cctttaggaa cagaaggaca aggagtagtt
    47101 gcatcaagaa gtaaatataa acgcacacag ttatgtgttc ctttgggact aggtgcaaaa
    47161 tatgcaattg accgtcagat aagcgtaggt cttgaattag gtttgcgtta tacctttact
    47221 gactatattg acgatgtaag caaaagttac tattatgata ggggtggctc cttggttgaa
    47281 atcctggcag atcgaagcct tgacgataac tcacatgttg gtgaacagcg tggtgacccc
    47341 agatggaatg atgcttatgt ttttgctatg tttaatattt gttataaaat aagaacaggt
    47401 agagtgaatt atccaatgtt ctgatgtaat tttcagaata tttttccgtt tttattgctg
    47461 tttttcagcc atgcgcaaaa ttttaatctt attttttata gtcgtggttc ttcaaccaca
    47521 aaaatcatcc gcacaaagca gtgagttagg gttactactt ggagtagctt cttataaagg
    47581 cgaaataaat acccatcttt ttaatcctaa tgcatttaat ccggcagcag gtttatatta
    47641 caaacgatgt ttaaacagtt tttggtcgtt tcgtttaggg cttacttatg gacaaataac
    47701 aggaagtgac gccatagcca aagaggattt taatcagtat cggaatcttt ctttcagaag
    47761 cgatatttgg gatgccagcg ctttgtttga gtttaatttt tttcctttcc agacagcttc
    47821 agaacatacc tctaaagtgg caccttattt agttgcagga gttacattct ttcatcacaa
    47881 cccacagggg ttcatcaatg gctcatggca cgatttgcag cctcttggca cagaagggca
    47941 gggtagtgga cttcccaaca cgacaggaag gtataagcgt gtgcagtttg cttttccaat
    48001 gggtggtgga ataaaattta aattgtcaaa caggttttcg ctttctgttg aggctattgc
    48061 cagaagaacc tacaccgatt acttggatga tgtgagtaca atttatccta atcagaatga
    48121 gctaaggaat acaaatggtg ttcttgcagc agaactctcc gacagaacta tttttgcggg
    48181 tacagatata aatgcaaaac gtcaaagagg aaatgcctca gataaagact ggtacatgat
    48241 gggtggcttc actatcaatt ggacattatc aaagaaatat actgataagt gcaagccttt
    48301 ccgtactaag ctgagataaa atttttcaaa gactacgaaa actctcaaat cacaatgcca
    48361 gagagatatt atccaaacaa agaacattta gaatatcaca acgacaatat tattatccga
    48421 tgaacagcat aaaaacgatt gatacagagt atttgcgttc agatcgaacc attgacacta
    48481 ttttagtcaa acaaggagta ttaagcaaaa ctctttgggt ttataacttc gagggtattc
    48541 actttagggt ttttgaaagg tttttggatg tttattattt ttcaaactac tcatttgaag
    48601 ctaaatacga gtttaacagt gaaaaagagt tagatgattt tttattgaat tttcacttaa
    48661 attaaaatgt ccgaaaccca aaatatagaa tataaaagca gttggcgtga cgagtatctg
    48721 aaatggatat gcggttttgc caatgccaat ggtggtgtaa ttttcattgg taaagatgat
    48781 gcaggaaagg tggttggtat ttcagatgcc aaaaagttaa tggaagaaat cccaaataaa
    48841 gtaaaagaca cgttgggcat attggtagat gtgaatttgc acaagagtaa gcaaggcggg
    48901 tttgttgaaa ttgtggtaga gccatatcct tatcctgtaa attacaaagg tcagtatcat
    48961 ataagaagcg gaagcactaa acaggaattg aaaggtgctg cattaaataa atttttgttg
    49021 cagaaaaaag gtaagcgttg ggatggggta ccagttccca aagtttcggt aaaagatttg
    49081 aagcaggaaa cttttgagtt tttccgtaag cgagctctca aaagtcaacg aattgatgaa
    49141 gacagcttaa ccgacagtaa cgaacatttg attgagaatt tgcagttgaa agaaagcgaa
    49201 tatttaaatc gtgctgctat tctgcttttt cattccagcc ccgaaaaatt tgtaacagga
    49261 gcatacatca aaatcggtta ttttcaatcg gatgatgatt tgaaatttca ggatgaaatt
    49321 cacggaaacc tgtttgagca gattgagaaa gctatggatt tgctgtttac gaaatacata
    49381 aaagcagaaa tcagttacga aggcattaac cgtgtagaga aatttgaata tccgaaagaa
    49441 gcagttagag aagctttaat gaatgctatt gcacataaag actattcggg cggtgtcccc
    49501 attcaaatta gcgtttatag cgataaagtc attttctgga acgaagggca actacccgat
    49561 aattggacag tgaaaacact tttagagaaa catgcctcac gaccttacaa tcccgacatt
    49621 gcaaatgctt tattcagaag tggctacatt gaatcgtggg gacgaggtac cataaaaatc
    49681 atcaaggaat gtaaacaggc aggaatcccc gaaccagttt ttagttatga ttcaagcgac
    49741 atttctgtgg agtttagaaa agatatttac aatgaaaa
//
LOCUS       DAOQNP010000013        49103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_205_length_49103_cov_8.011886, whole genome shotgun sequence.
ACCESSION   DAOQNP010000013 DAOQNP010000000
VERSION     DAOQNP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 49103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49103
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205_length_49103_cov_8.011886"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKY09_03220"
     CDS             <1..972
                     /locus_tag="PKY09_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB11895.1"
                     /translation="LEKVYDKIVSKGAALAGIKKALFFWALNLGLRYELKGANGWWYE
                     FQLRLANKLIFSKWREALGGNIKAIASGGAALQPRLARVFHAAKIPVLEGYGLTETSP
                     VIAVNNFQPDSIKFGTVGTVLENVDVKIAADGEILCKGPNVMLGYYNSPELTAEVIDA
                     EGWFHTGDIGVFEDGKFLKITDRKKEIFKTSGGKYIVPQMIENKLKESRFIEQAMVIG
                     ENQKFASAFIVPNFAFIREWAAKKNIAVNSNTEIAASSVVKKRIAEEVEVVNKTLGHY
                     ETIKRFELLDREFSIERNEMTPKLSLRRKIILDNYKEKLAKIYKDTE"
     gene            987..1820
                     /locus_tag="PKY09_03225"
     CDS             987..1820
                     /locus_tag="PKY09_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB11896.1"
                     /translation="MITRKQPVTMMYSKALIKPDGEVLNYSDTSSGNEVLVFIHGYPF
                     DQSIWKEQVIALSEQARIITYDVRGYGSSSKGKEKFSIDLFADDLIFLLDSLQVNKVI
                     VCGLSMGGYIALNLVKRYTDRVKGLVLYDTQCYADNAIAKAKRYENIALVKNGGKQKY
                     LEDMCKILFSPVTFQQKYEIINQTLNLMMKVDDDVLMRTLEAMAEREESCSILAAIKI
                     PVMIFCGEHDMLTPPEKSEFMQQQIQGSVLHIIENSGHLGHLENKEAFNQHLNNFLKS
                     F"
     gene            complement(1817..3076)
                     /locus_tag="PKY09_03230"
     CDS             complement(1817..3076)
                     /locus_tag="PKY09_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNB11897.1"
                     /translation="MRIKSILITLLAIANLRANAAQILIPMDDTQANHLKAYGIAYWV
                     LQSGIEINWMLNYRGGSFMFIQNPKFESELKLRGVSYNVISEGQANQIVSEILDPQVN
                     QDVVKLEKVPKICVYSPKDKRPWDDAVTLALTYAEIPYDVIYDEEIIQGKLALYDWMH
                     LHHEDFTGQYGKFYNMYRNATWYQEQQRSMESMAKQLGFAKVSQMKLAVAKRIKEFVA
                     GGGFLFTMCSGTDTYDIALSAEGLDICESMYDGDGTSSNYNDHLDFSKTFAFRDYTLS
                     VNPLEYRFSNIDVTTTRRVPRDEDVFTLFDFSAKWDPVPTMLCQDHMQVIKGFMGQTT
                     AFNKAYIKPDVIIMGENKAANEAKYIHGDFGKGTWTFYGGHDPEDYQHMVGDPPTDLN
                     LHPNSPGYRLILNNVLFPAAKKKKQKT"
     gene            complement(3092..4987)
                     /locus_tag="PKY09_03235"
     CDS             complement(3092..4987)
                     /locus_tag="PKY09_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB11898.1"
                     /translation="MRKLLLFFILSPFSGFSQDNVHVHCKRIISDNSKLHSLNKVSTT
                     GSDFDVKYNRFNWVINPDSVYISGNVFTLFEVKSSLSKLEFDFSSALTIDSILYQSTL
                     LPFTSSSDIITILLPGIISTGQLDSVEIFYHGVPPTSGFGSFNQSTHNGIPVLWTLSE
                     PFGASDWWPCKNSLTDKVDSMDVFVTTDTAYKVASNGKLISEISSGNQKITHWKSNYP
                     TASYLIAIAVTNYAAYSDYVPMAVGDSLQVLNYVYPENLSSAQLQTPDIIPIIQLYDS
                     LTIPYPFRNEKYGHAQFNWGGGMEHQTMSFMVNFNQDLMAHECAHQWFGDLITCGSWE
                     DIWLNEGFATYFEGLTKEHYIPSLWYNWKRQKINNITSQPDGSVFCTDTNDVSRIFNG
                     RLTYNKGSYLLHMLRWQLGDSVFFNALTNYLNDTLLKYGFATTPDLIAHLQNTSGQNL
                     STFFNQWYYNQGYPSYHITWNQNNPAVWLKIDQTTSHPSVAFFEMPVPVRFYGNGTDT
                     TLVFNHTFSGQQFTAAVPWQVDSVVFDPDLWILSANNLVTTGIETKEISNEKQQIYPN
                     PVHDFINVVCKTNCNYTITDANGKIISKGELHQSNNRIKLNSKPGIYFLNTSAENKWL
                     CQSFIIQ"
     gene            complement(4987..5658)
                     /locus_tag="PKY09_03240"
     CDS             complement(4987..5658)
                     /locus_tag="PKY09_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB11899.1"
                     /translation="MSCISCKKLWSIPALEWLIILITITLGLLWTSCNEDRPLRTLPV
                     YGMQDEQNKSNHKIGPFSFIDQNGDTVTDKDYSNKVYVTDFFFTTCRSICPVMTTQMQ
                     RVFEHYKSNPDVMFLSYSVNPEYDTPAVLKEYADKHHADAKKWHFVTGDKQKIYDLAR
                     ISYLVTATKGDGGPDDFVHTQNFALIDKEKRIRGYYDGTDSIDVNRLITEIDLLLREY
                     KYADK"
     gene            complement(5655..5933)
                     /locus_tag="PKY09_03245"
     CDS             complement(5655..5933)
                     /locus_tag="PKY09_03245"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNB11900.1"
                     /translation="MRKVIVALLVLFTACQPKTSEPEQKTAPALEEKEFPAKPDIALA
                     SDIDPVCKMSVKEEFSDTAMYDGKIYGFCASACKDDFLKEPLTYLEEK"
     gene            6031..6531
                     /locus_tag="PKY09_03250"
     CDS             6031..6531
                     /locus_tag="PKY09_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB11901.1"
                     /translation="MIKQVYTVLTILMTFGMINQVNAQEKVAIYHPEKNTFEQIKQAE
                     ITAQKEGKHVFLMIGGNWCRWCRMFDAFSKTDLSVDSAFKANYVIEHINYSKENKNDS
                     LMQQLEFPQRFGFPVFVILDAQGKRLHTQSTGYLEQGEGYNKESVIEFLNQWSPAALN
                     PANYKK"
     gene            complement(6541..7902)
                     /locus_tag="PKY09_03255"
     CDS             complement(6541..7902)
                     /locus_tag="PKY09_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB11902.1"
                     /translation="MFRKIVLSLLLLFIGLQLSADTPLLKRVVCSYDDTTRLSFVSNT
                     DVFAERWDTLQQVKFWQEVIRQSADTALINVASTRCILHKTPFEDWKCQSESEKTAYK
                     KFINLANGLDTNTTLYVTFGKKEFYEYKRVIPVIGKSVEIFKNNNVDPWYAQTILLIE
                     NPGKNHNKSYVGANGPFQLMKSVAIKFGLKVNKHVDERTDLNKSAYAASQLLKSSCIP
                     KVKGLLIEKNIPFNEDDLWFRLLVMHAYHAGPGNVACVINELNPKKGGIELFTQIWKT
                     ECGGFKNESQNYSQIALANIVIFERFLQSNKDSLWLVEGEKMMADYKKNKTPGIAGIN
                     KLKQSLELYSEDLMDGTISADFFISRMTSIQKELATYKVKKTNEEETNLSMTQYLAIG
                     KQLIRKRHLDDAIKILKLNIQHYPNCVTAYDSLSRIYKIQGNRQMALFYNQKGASIKN
                     PSY"
     gene            complement(8165..9613)
                     /locus_tag="PKY09_03260"
     CDS             complement(8165..9613)
                     /locus_tag="PKY09_03260"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNB11903.1"
                     /translation="MIRQIKKTAGILAIAAIGGFTSLAIYKQTEKPVLFGQQENVVPV
                     KRVSITGNTETAMFDFTEAAEKTVPAVVHVKTTYPSQPVSGNFFNPFNFWGAPGYGAP
                     QQPQQSAGSGVIISDDGYIVTNNHVVDNADKVDVTLDDKRTFTAKVIGKDPSTDLALL
                     KVDQTGLPYSKFANSDDIKVGQWVLAVGNPFNLTSTATAGIVSAKGRNIHILNDQQFP
                     IESFIQTDAAVNPGNSGGALVNLNGDLVGINAAIASNTGSYTGYSFAIPANIVRKVTK
                     DLLEYGKVQRGFIGVTLRDIDQQLAQTKGLKSMQGVYIDGVVSGGAGQAAGIKPGDVV
                     TKVGETTVNSASELQEQIGRFGPGDKVNLTLNRDGSEKNYQIVLRDNSGGERVERANN
                     NVNMMGAEFGEVTDKEKKQLGISNGVKVAGLQSGKLRSAGIREGFIITSIDNKKINSP
                     DDIESALANKKGGVLIEGVYPNGMRAYYGFGL"
     gene            9778..10572
                     /gene="dapF"
                     /locus_tag="PKY09_03265"
     CDS             9778..10572
                     /gene="dapF"
                     /locus_tag="PKY09_03265"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNB11904.1"
                     /translation="MHLKFSKYQGAGNDFILIDNRSSKFSTLKPKQIAILCDRHFGIG
                     ADGLILIQHSRDADFEMVYYNADGNIGSMCGNGARCAVLFGKQIGVAGKKTQFMAYDG
                     LHTAKINAVNNFASAIVSVSMSDVSEIKKHNGNFVLNTGSPHYVKFVDDVEKIDIVKE
                     GRRIRNNNTFKAKGINVNFVAYKSKASNIRTYERGVEDETLACGTGITASAIAAHYSG
                     LTKSTSVNMIARGGSLNVTFTVKDNQYNKIVLTGSAMKVFDGEIKI"
     gene            complement(10562..13015)
                     /locus_tag="PKY09_03270"
     CDS             complement(10562..13015)
                     /locus_tag="PKY09_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB11905.1"
                     /translation="MIKKFSLLLLALCFSLYSNAQFDLSGVVSTNDGKKVDNAVITLK
                     ESLTRHFSDEKGRFTFFNLKAGKYTLQVYQLGYHEYSSEINLTSNQHVEVVLFKKAFV
                     TDEVIIQSLKMKNKIPIAQNNLNKNEIEEINSGKDVPEIMDQLTGVVISSDAGAGVGY
                     TGMRVRGSDATRINVTINGIPINDAESQSMYWVDMPDLLSSTNQIQLQRGVGTSVNGA
                     AAFGASINILTDHLSEKPFAETNLSYGTFNTKKVNLIAGTGLLNNHFAFETRLSSIQS
                     DGYVDRATSDLKSFYFSGGYFGQKSMLKAIMFSGIEKTYQSWYGVPEARYKNDTQGMM
                     DYISRNGLDNEDAANLLESGRKYNYYLYPGQTDNYQQDNYQLHFSHQINNLFTLNVAA
                     HMTRGKGYYEEYKKNAKFGDYGLPPIQIQDTLITNTNLVRRKWLDNYFYGMIYSLNYE
                     NKNIEIKTGGGINQYDGNHYGKIIKGDFIPLASENYTYYQNNGLKNNFNHWLKGTLHL
                     GEHFIAYADMQYRYVHYRFTGFDDFATNANQIAAYHFLNPKAGVTYLRNDNESGWLSI
                     AVGNKEPSRDDFTESTAKSRPKPENLIDFEAGYSIEIKKLKLSFNAFYMQYKDQLILT
                     GKINDVGNYTRQNVKNSFRRGLEAEFNYDASKFLVVSGNVTLSENKILNFKEYVDQYD
                     ADFNYIGQQEIIYKKTHIALSPSIIAAAGVEIKPLKKLSIKLTEKYVGKQYLDNTSSE
                     EKSLKSFAYTNISAHYTFKIFKNIETDAGVSVFNLFNEMYSSNGYTYGYLYDGMRVNE
                     NFYYPQAGRHTMISAKFRF"
     regulatory      complement(13064..13164)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13323..14186
                     /locus_tag="PKY09_03275"
     CDS             13323..14186
                     /locus_tag="PKY09_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNB11906.1"
                     /translation="MRQLRIQKSITNRESQSLEKYLQEISREGMITADEEANLARRIR
                     EGDEEALSKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAASRFDETRGFKF
                     ISYAVWWIRQSILQAIAEQARIVRLPLNQVGSLRKINKVFSKLEQEYEREPSAEEVAE
                     IMEVPIEKISDTLKSPGKHVSMDAPFAGNEEATLLDVMQNNDAPDTDYGLMNESLRKE
                     IDRSLKTLTERERDVVKLFYGIGVSHSFSLDEIGAKFGLTRERVRQIKEKAIRRLRSG
                     SRNKLLKDYLA"
     gene            14234..15382
                     /locus_tag="PKY09_03280"
     CDS             14234..15382
                     /locus_tag="PKY09_03280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11907.1"
                     /translation="MAQKKVIISVINDIATDQRVRRTASVFSELDYQVLIVGRKFPYS
                     LPVYEMPGLSVKRFRLLINTGPLFYALFQIRLFLFLFFKKTDILYSNDLDTLLPNYLV
                     SRLKGCALIYDSHEYFTGVPELQNNHFARKVWTKVEAFIFPKLKNVITVNQSIANLYH
                     QQYGIDVKVVRNVPDKTDSINFDVMQWKKSVGLSNDKKVFILQGAGINIDRGAEEAIE
                     AMKRVDAVLLIIGGGDVFHRLQELAIKQKVDQKVIIHPKVAREELIAITSSCFAGLTL
                     DKDTNINYRFSLPNKLFDYIHAGIPVIASDLPEVAAIVNEYKTGIVLPKYTTQAIAEA
                     MNRLIIEKELYINYKENCSIAATDLCWQNEKKILIEVILCSEKKTNKL"
     gene            15391..16515
                     /locus_tag="PKY09_03285"
     CDS             15391..16515
                     /locus_tag="PKY09_03285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11908.1"
                     /translation="MRNSGKHLHIISFDIPYPANYGGVIDVFYKLRALVSAGVKVHLH
                     AFQYGRQSSKILEELCESVHYYQRDVSKSQLFLKEPYIVVSRSSEQLIKNLLSDDHPI
                     LFEGLHSTHYLNDERLKKRKKIVRTHNIEHEYYKNLAKAESNIFKRYYFYNESSKLEI
                     YESILLNADIIAAISPNDTKYFNSKYGNAHYIPAFHSFTKVNSRVGKGAYAFYHGNLA
                     IGENDMAALYLVQEVFKNSPVTLLIAGSKPGVNLRDAVQRKNNVTLLSDLNTKQINDL
                     VLDAHINVLPTFQPTGIKLKLLSALFSGRFALVNTPMVQNTGLEQLCTVADTPEAMRE
                     ALAAMIKQEFTQIDISLREEILMENFNNKANALKLKDLIF"
     gene            16547..16912
                     /locus_tag="PKY09_03290"
     CDS             16547..16912
                     /locus_tag="PKY09_03290"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNB11909.1"
                     /translation="MLQVTGILKQKFAEQVVTDKFRKREFTLTIDAKSSYPQIVIFQL
                     ANDKCRLIESFQPGDELSVSFNIKGREWKSPSGEIKYFTSLDAWKIERTSAGEAPMES
                     PMPSMSDLPQEDYKDDLPF"
     gene            16973..17764
                     /locus_tag="PKY09_03295"
     CDS             16973..17764
                     /locus_tag="PKY09_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNB11910.1"
                     /translation="MKNDFKNEKWSLVKPPFRFTNNLKIEVSNFGRVRTSTKIAENRV
                     LKGTLVNGYPIVRFKLFKKRSDEAQKQLDQKRRKIEQLTGKLRDVKSELKSHKKNSPA
                     YNSHKKKIDLVTAELNGIKEVYRKAFRKDELERTINYAPLVHRLVADNFVKQSSKKHD
                     IVAHIDYNKSNNHYSNLKWMTQEDNIEHQKKNPVVIKEKKKRIGKRPKNASGYKLNEK
                     KVAEIKKRIHKGEALSKISDRFHVSETQLLRIKRGINWGDVKISR"
     gene            17806..18582
                     /locus_tag="PKY09_03300"
     CDS             17806..18582
                     /locus_tag="PKY09_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNB11911.1"
                     /translation="MQHIFFLLSKVLDFLLSPLSWIVILLLISIITKNKKRSRKCLYW
                     AIGALLFFSNPFVLNLVMHAWEVQSVPMDSIHKQYDAVIMMGGAIRYVNGQMQRPVFG
                     SGADRYVQTMELFYRNKARHIIISSGSGSLVYTDVKEADLLRDQMIRIGIPADKLIAE
                     NQSRNTYENAKNTAEIIQKSDFKPPFLLVTSAFHMRRSMLCFQKQGIDVIPFAVDQHS
                     GQIMFTPDKLLLPSPEVLLDWDLLFHEWFGIAVYKVMGYL"
     gene            complement(18592..19326)
                     /locus_tag="PKY09_03305"
     CDS             complement(18592..19326)
                     /locus_tag="PKY09_03305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB11912.1"
                     /translation="MKKMLLRMIFKILDKYFKSQNYVNAQNKYFKSHYAFDIETQLRI
                     RALQSTCEFIEKNMSHLPMFKNKQDALDFSLKQVKISGLYCEFGVYKGASVNHIAKVT
                     KQEVHAFDSFEGLPETWLTTHKEGHFALEKLPHFEPNVVVHKGWFNETLPDFTEKYKA
                     PLAFLHIDCDLYSSTKTVFEALNKQIIKDTIILFDEYFNYPHWQQHEYKVFKEFTEAN
                     KISFEYLCYSSNTFGSKVAVRILDRE"
     gene            complement(19378..19767)
                     /locus_tag="PKY09_03310"
     CDS             complement(19378..19767)
                     /locus_tag="PKY09_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010178301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNB11913.1"
                     /translation="MKAVQKKQKVKMEFPMRSSPKILYGFLSTPSGLSEWFADDVTIR
                     EGIYTFHWDGDEQQAKMTHKRENQMVRFKWIDDSDDAFIEFEITTDELTSDVSLMVSD
                     FAVEDDIEETQRLWDTQIHNLRHLIGS"
     gene            complement(19832..21643)
                     /gene="typA"
                     /locus_tag="PKY09_03315"
     CDS             complement(19832..21643)
                     /gene="typA"
                     /locus_tag="PKY09_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNB11914.1"
                     /translation="MQNIRNVAIIAHVDHGKTTLVDKILHQTQVFRQNQQTGELILDN
                     NDLERERGITIVAKNVSVQYKDVKINIIDTPGHADFGGEVERVLTMADGVILLVDAFE
                     GPMPQTRFVLQKALALGLKPIVVINKVDKENCRPDEVHESVFDLFFHLEATEEQLDFP
                     TLYGSSKHGWMSTDWHTETTDITPLLDAIIEKTPPAKSLEGTPQMLITSLDYSNYVGR
                     IAIGRIVRGTLKENMPVTLMKRDGSAKKSRIKELFVFEGLGRKKVDSVSSGDIAAIVG
                     VDDFEIGDTIADFESPEALKSIAVDEPTMSMLFTINNSPLFGKEGKFVTSRHLRDRLY
                     KETEKNLAMRVEETDSPDAYLVYGRGILHISILVETMRREGYELQLGQPQVLYKEIDG
                     IRCEPVEHLVVDTPTEVAGKVIELATQRKGELTSMLPKGDLQRLEFNIPARGLIGLRN
                     AVLTATAGEAIMAHRFNSYEPVKGAIPGRNMGVLLAKEAGSSTAYSIDKLQERGAFFI
                     DPGETVYGGQIIGEHIRPGDLVVNVCEGKKLTNIRASGSDDNVRITPKIKFSLEEAME
                     YIEKDEYLEVTPQNIRMRKIYLDENERKRMSKKAEMA"
     gene            complement(21721..23163)
                     /locus_tag="PKY09_03320"
     CDS             complement(21721..23163)
                     /locus_tag="PKY09_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB11915.1"
                     /translation="MAKLKLSENNLQTEMLDHSLFRQIASLCEKHNLKAFVIGGFVRD
                     CFLKRSSKDIDIVVNGDGIAVAKELAVTLDGEFAFFKNFGTAMVKSEGFEIEFVGARK
                     ESYNQHSRKPKVTPGTLEDDQLRRDFTINTLAFSLNRDDYGNLIDPFNGMQDLNDKII
                     RTPADPVTTYSDDPLRMMRAIRFATQLNFSIEEKSLAAIHSERNRINIVSKERITDEL
                     NKIIASPLPSTGFKLLMNTGLLELIFPQLYNLKGVDYFEGKGHKDNFYHTLEVLDNIA
                     KVTDNLWLRWAAILHDIAKPATKRFEAGHGWTFHGHEDKGSRMVPPIFRELKLPLNEK
                     MKYVQKLVLLHLRPIVIAQEIVTDSAVRRLLFDAGDDIDDLMLLCQADVTTKNEYKKA
                     KYRNNFELVVQKLKEVEEKDRIRNWQPPVTGQLIMDTFGLAPGPNVGIIKDFVREAIL
                     DGQCPNEPQAAFELMLKKGIELGLTPQKNS"
     gene            complement(23135..23722)
                     /locus_tag="PKY09_03325"
     CDS             complement(23135..23722)
                     /locus_tag="PKY09_03325"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB11916.1"
                     /translation="MKNSNSEIFSDDIKSCLETLRQGGLIVYPTDTIWGIGCDATNDA
                     AVEKIFTLKNRSESKNLITLVSDEAMLNRCVQHVPAVAWDIIEYSEKPVTIIYEKISR
                     ISEKAIADDGTAAIRLVKDPFCKMLIHKFGKPIISTSANLSNMPSPAVFNDIEDSILT
                     GVDYVVKFRQQETAKHTPSSIIRILNNGEVKVIRK"
     gene            complement(23793..25094)
                     /gene="murF"
                     /locus_tag="PKY09_03330"
     CDS             complement(23793..25094)
                     /gene="murF"
                     /locus_tag="PKY09_03330"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNB11917.1"
                     /translation="MIAHLHSLLLSSTGVCTDTRKLIKGNIYFALKGPSFDGNKFALQ
                     AMQNGALAAVVDDSSIKGENIYLVENVLTTLQHLATYHRQALKTPVIGLTGSNGKTTT
                     KELISRVLGSSYNVLFTEGNLNNHIGVPLTLLRLNSEHQIAVIEMGANHQGEIQELSK
                     IALPNFGLITNIGKAHLEGFGGIDGVIKGKTELYRHLMENDGVIFCRRNNELLTNNLN
                     HYQKVIFYSTQPDVCIYGEISAPASVVKFNWFENHNRIDEITTHLTGDYNLENLVAAV
                     CIGRYFNVTPEKIKSAIEGYIPENQRSQIEISGSNTIIIDAYNANPSSMEAALKNLSE
                     NYDGNKVIILGEMLELGQDANAEHQHIVDLLSSMEKDLLILVGENFLHTTLNDHALRF
                     KTSEDACNYLQNHAINESTILVKGSRGSKMEKVLDGIRNHN"
     gene            complement(25168..26616)
                     /locus_tag="PKY09_03335"
     CDS             complement(25168..26616)
                     /locus_tag="PKY09_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNB11918.1"
                     /translation="MNLRKNAKTLAFVALAVMATNACKQKEKSSTTGWKYNDEKNGGF
                     EVTPYTEQETGPGLILVEGGTFAMGRTEQEIPYDWDNIPRRVTVSSFYMDETEIANVH
                     YLEYLYWLDRVYGTDYPEVYKKALPDTLVWRSKLGYNEPMVELYLRHPAYQRYPVVGV
                     NWLQANDFASWRTDRVNEQILVREGILRVNPAQVNEDNFNTDAYLAGQYEGLVKSKLQ
                     DLNPNRDERNVRMEDGILLPRYELPTEAEWEYAALGLIGNTVYERVTDRKIYPWNGHV
                     VRNSSEKYKGEMLANFKRGRGDMMGTAGALNDNADRPAPVFSYLPNDYGLYNMAGNVS
                     EWVKDVYRPLSPEDMDDFNPYRGNVFVTPLLDEEGQYAEKDSLGRMVWREVDEKENES
                     RRNYTKADYINDLDGDEPDNIEYKMGGTTLIDDNAHVIKGGSWKDRAYWMSPGTRRFL
                     DQQQSTDWIGFRCSMVRVGSPVGFNQKASKKG"
     gene            complement(26658..27656)
                     /locus_tag="PKY09_03340"
     CDS             complement(26658..27656)
                     /locus_tag="PKY09_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNB11919.1"
                     /translation="MIKRIVLTMIISLELAICFGQDPQFSQFYANPIYLNPAFAGSVR
                     CPRLALNYRNQWPGLNKTFITLSASYDQHVEALSGGLGLIIMNDKAGNGTLNTTNFSG
                     IYSYNLNISRNFSIRAGLQATYVQEKLDWDKLTFGDMIDPRYGYVFETQEVRPAEKRG
                     FADFSAGVIGYSSKVYGGFAVHHITEPDQAFIVEGSSPLPRKYTAHVGALLPLKGAST
                     YRGKLEGTFISPNFLYQQQQGFNQLNFGLYVIKSPIVGGLWYRGYTGKKFLSSDSFIA
                     LIGLQKDMFKFGYSYDVTVSKLNNNVTAGSHEISLGLQFECRPKKKRFKAISCPTF"
     gene            27863..31690
                     /gene="porU"
                     /locus_tag="PKY09_03345"
     CDS             27863..31690
                     /gene="porU"
                     /locus_tag="PKY09_03345"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNB11920.1"
                     /translation="MKSFFTLFFLIGLTGVAVAQQDESIKRTYSIDWRSNVVLNGEDR
                     LNFTNCFYKQDSKMPYFSTGLPLADITKVAEVSIENPVYEPLISNNTIDASSEILPET
                     KLSYFKGKPSLYIEFVPVRKNAVTGQTEKLVAFNLKVTSGQQSQKIGAVSTQVYAANS
                     VLASGEWYKISVTEDGVYKITYSFLKDHLKIDLSASSPQSFRLFGNGGGILPLLNSQF
                     RYDDLQENAVFVNDGGTAGKWDDQDYVLFYGQSPVQWYYNSSQNRYYHQANYYSDSTF
                     YFITFNGGTGNPKRIQQSASLSVTPDFVVNSFDDYQYHELDKTNFVKSGREFYGESFD
                     INLNQTFSFNFPNILQQTVTIKTVFAGHSPGVTSSASARYQNITLFSGTYTTGSQYTD
                     DYAMERTTTSTFLPSAGDVVNIDFTFTPANTECIGFLNYIELLAKRALTFAGNGTTDQ
                     MFFRSMESSGLGKTAQFNIASAPASMTVWNITDRINVRQQLHNNGSYITQVDSTQQFI
                     AFGGNNFLTPGAIGAVANQNLHSLPQADLIIVTHPLFEDQANRIADMHRTLDNMSVNV
                     VRTDLIYNEYSSGAQDIAGIRNFVKMLYDRGINNGTEPKYLLLFGDGSYDNKYRRPNN
                     TNFIPTFESENSYSFLSSFVADDFYGLMDDNEGLCSSNEKIDIGVGRIIAQNISEAKT
                     VTDKTIQYASLPQNSGCPGCDGSGGTLGDWRNVVTFIADDEDGPIHVPPAESFANYLN
                     TNHKTYNVDKIYLDAYKQISTPAGQRYPDVNDAINKRVNKGSLIINYVGHGGETGWAE
                     ERVLTNDDINSWTNINKLPVFITATCEFSRWDDPARTSSGEKILLSATGGGVALFSTT
                     RLVFASSNEVLNRALIKFMFDDPTYRLGDIMVASKTDPGNLNLNTRNFSLLGDPALRL
                     HYPKFNIEATQINTQPIIALNDTLSSLEKVTIAGRIVKGGQLQSDFNGFMYPTVYDKA
                     SSITMLRNDASSPNITFKLQKNILYKGKATVKNGEWSFTFIVPKDISYQFGLGKLSFY
                     AENGVEDGAGYFDSIVVGGSSANIISETDGPGIKLYLNDERFAFGGTTNEKPTIVCLL
                     SDSTGINTVGNGVGHDITAIIDGKVDPVYVLNDYYEADLDSYSSGRVAYPLSKLSEGR
                     HTLKVKAWDILNNSSEAYTEFVVASDASLALKHVLNYPNPFTTHTQFTFEYNKPCEDL
                     EVQVQIFTISGKLVKTISKKIITPGTRIDDLTWDGRDDFGDRIGRGVYIYRVKLNTAD
                     ESASETQKLVILK"
     gene            31817..33019
                     /gene="porV"
                     /locus_tag="PKY09_03350"
     CDS             31817..33019
                     /gene="porV"
                     /locus_tag="PKY09_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNB11921.1"
                     /translation="MRYKLLYGALSLAVLAIQNQASAQNDKANLLGQINTITTAVPFL
                     MISPDARAGGMGDAGVASSADVNSIHWNPAKLVFAEKKMGVGISYTPWLRKLVPDINL
                     AYVSFFTKPNDVSAIGASLRYFSLGNIDFTSETGDPMGSYKPNEFAVDVAYSRKLGDE
                     FSGGIALRYIHSSLTGGITVGGNATKAGNSVAADVSFFYKTNLEISGKDAEFGAGLAI
                     TNIGSKMSYTDKGTKDFIPINLRLGPSLKIKLNDYNELAFLVDFNKLLVPTPPVYSDS
                     TIAGEENPIIFAGKDPNVGVAQGIFQSFGDAPGGGKEELREINIAGGLEYWYDKQFAF
                     RAGYFYENKTKGNRKYFTLGIGLRYNIFGLDFAYLIPTEQRNPLENTLRFSLSFNFDS
                     IKGSDSNE"
     gene            33023..33502
                     /gene="ispF"
                     /locus_tag="PKY09_03355"
     CDS             33023..33502
                     /gene="ispF"
                     /locus_tag="PKY09_03355"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNB11922.1"
                     /translation="MNFRVGFGFDVHQLEPGIPFFLGGVRIEHHQGAKGHSDADVIIH
                     AICDALLGAAALGDIGKHFPDTSNEFKGIDSSILLAKVVSILKEKSIAIGNVDVSVVL
                     EKPKIAPYIQAMREKLASVMQIDLDCVSIKATTNEKMGFVGREEGVAAYAVALINRQ"
     gene            complement(33489..33902)
                     /locus_tag="PKY09_03360"
     CDS             complement(33489..33902)
                     /locus_tag="PKY09_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNB11923.1"
                     /translation="MNTDKKHDILTPEDVVLMVDTFYSAIREDDLLGPIFNNNIQDRW
                     PEHLKKMYGFWRSILFNEPLYNGSAFEAHRQLPIEMKHFKHWLKLFEVTVDSLFEGRN
                     ADTAKTRAGKIAEVFYYKIESERGNDPQNFSLTAG"
     gene            complement(33932..35293)
                     /locus_tag="PKY09_03365"
     CDS             complement(33932..35293)
                     /locus_tag="PKY09_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB11924.1"
                     /translation="MFAVVDIETTGGSLKQGSRITEVAVVIHNGTTIEHRFSTLINPE
                     TDIPSFIIGLTGITNELVENAPCFKEIAGQLLQLLDGKIFVAHNASFDFSFIKAEFKR
                     CGIDFNAEVLCTCSLSRKFFPGFGSYSLGNLTKNLGIVLEGHHRAMNDAIAASEILEK
                     LVSERGLEHTLLHRSVPIKNTEIPYELINKLPEVPGIYFFTDSNHEILYIGKAKNIKK
                     RVTTHFRNNNSKSKKIKALLHDICYMNTGCELLACLLEIQYIKEYQPFFNRAGKSEKN
                     FGVYINANLNGLLGFTINDIAKASLKPLRYFGSRKAADEFLSHITAKYNLCRCINNLE
                     NYSENCFYKQIKQCNGTDLDSHEKDNYNNRFNDAYFEVVDDGLHDAALAYPMADNDEW
                     VYVFRQNGSIGYYFGKKATTFNTNQIISVTFLKNHLESHRIFNSFLKKPSARRIKIVS
                     DES"
     gene            complement(35314..36270)
                     /locus_tag="PKY09_03370"
     CDS             complement(35314..36270)
                     /locus_tag="PKY09_03370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNB11925.1"
                     /translation="MHKILIVQTAYIGDVILATGLIESIHQAFPEAQIDFVLRKGNES
                     LLKNHPFVNKLYIWDKKKNKFSNLIKLIGQIRKERFDLVVNLQRFFSTGLIALCAKSK
                     NKRGYDKNPLSFCYHKKTEHFIGKNHETYRNFSLIDDLKNIHYQKPHLYFTAEEIQKV
                     QPLKSQPFVCMAPGSVWFTKQLPIAKWAELILTISDNIKIYLIGAPVDAALCNEIRLL
                     SSHPKIEILCGKLTLTESAALMQHAEMNYVNDSAPLHLASAVNAPVTAFFCSTVPEFG
                     FGPLSDKSVIKQVGEMSCRPCGLHGYKSCPLGHFDCAYKIEI"
     gene            complement(36282..37097)
                     /locus_tag="PKY09_03375"
     CDS             complement(36282..37097)
                     /locus_tag="PKY09_03375"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNB11926.1"
                     /translation="MPVREKIVNDIWENRELLNDESNKNVIRSVIEDLDKGKIRVAEP
                     LNNSWNVNEWIKKAVILYFPIQKMAVHHAGPMEFYDKIDLKTGYEDLGVRVVPHAIAR
                     YGSYVAKGVIMMPSFVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQAAPVIIEDNCFIGSRCIVVEGVRVEKEAVLGANVVLTLSTKIIDVSGGAPVEYRGV
                     VPARSVVIPGSISKTFPAGTFQVPCALIIGKRKQSTDNKTSLNDALREYDVAV"
     gene            37183..37611
                     /gene="ruvX"
                     /locus_tag="PKY09_03380"
     CDS             37183..37611
                     /gene="ruvX"
                     /locus_tag="PKY09_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNB11927.1"
                     /translation="MPRILAVDYGNKRVGLAVTDPMQIIATALTTVHSSKAVEFISDY
                     CNAEVVEEVVVGKPVQMDNTPSEIEKNILLFIKKLSERCPNAKVVRYDERFTSKIAQR
                     TLLEAGLKKQQRQNKALIDQTSAVILLQSYLEHKRNFLKI"
     gene            37608..38168
                     /gene="def"
                     /locus_tag="PKY09_03385"
     CDS             37608..38168
                     /gene="def"
                     /locus_tag="PKY09_03385"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNB11928.1"
                     /translation="MILPIIAYGDPVLKKVGLPIDKDYKGLGQLIENMFETMYAASGV
                     GLAAPQVGISIRLFVVDTKPFAEDEPELENFKKVFINPQIIEEVGDEWKFNEGCLSIP
                     GIREDVMRKKIIHLRYQDEEFKTHETVFEGLAARVIQHEYDHIDGKLFVEKLSPFKKQ
                     LIKGKLRDITIGKVDVDYKMRFPIKK"
     gene            38174..38686
                     /locus_tag="PKY09_03390"
     CDS             38174..38686
                     /locus_tag="PKY09_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNB11929.1"
                     /translation="MLIFLIGFMGAGKSSQAKKLAARLSFEAVDLDELIEKANKQSVS
                     EIFNLKGEQFFRKVECETLRTISEKQNAVVATGGGTPCFFDNMQWMNEKGITVYLKIH
                     PGTVYHRIAPSKAKRPLISHINDADLMEYIMETMTERAPFYNQAKIQISGLNLKTEDL
                     VNALQPFLEQ"
     gene            38746..39255
                     /locus_tag="PKY09_03395"
     CDS             38746..39255
                     /locus_tag="PKY09_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11930.1"
                     /translation="MKILLFFLLLPTMLVAQTFQKNQGESNEDFVKRIAPQSANEVKY
                     MLSDTFNTSTQKVVYAYSIFEHGNLITNSDSTLTYFLCILVPTSVGAMDYNMQQFRMV
                     ASYKKKARIESAEIIKSGREKKLQVYIAELVRGPGGVPRDVKRTFVYKQEKESRMFTD
                     TFREVLGDY"
     gene            39266..40030
                     /locus_tag="PKY09_03400"
     CDS             39266..40030
                     /locus_tag="PKY09_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNB11931.1"
                     /translation="MNKAHELDFKIFGDDIQALEVELDPSEGVRAEVGMMMYMEDDIE
                     MQTNTGGGIFKGLKRMITGESFFITNYLNHGRQKRKITFAAPYPGKIIPVNPAEYGGR
                     FLCQKDGFLCAAMGIEINVAFTKKLGAGLFGGEGFILQELAGDGYAFIHSGGTIIKRE
                     LAVGERLRVDTGCLVAFAPTIEYDIKFIGGFKNVLFGGEGLFYAHLTGPGTVYLQSLP
                     FSRMADRIIAASKWGGAREENRGIGGLLGGFISGNE"
     gene            40162..44133
                     /locus_tag="PKY09_03405"
     CDS             40162..44133
                     /locus_tag="PKY09_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNB11932.1"
                     /translation="MASQFIPVDFNPFEEVIPDNIFTTSEEQREIWTSVQIGGDSASL
                     AYNESVTLYLKGLCNHQQLSEACKSVVERHQALRSTFSSSGETFQVHEHVNIEIPFID
                     LTDLSPTDKDARILHFSQREVSIPFDLMNGPVIRFAIFKISNADTALVITAHHIVCDG
                     WSIGIIMQDISKFYSAFVNNTQPDLEPVVPFSNYVKRQEERKLNGEYKRTEDYWLNLY
                     SKDVPTVDLPVNKARPAARTFNAKRFDITIDAGLVSDLRKLGAKNGSSYVNTLFAAFE
                     VFLFRLTSNPSLILGLPAAGQSASGMYAMVGHCVNLLPIKSFVDENINFGEYLKTRKP
                     LMFDAFDNQEFTMGSLLGKLPLQRDPSRIPLVPAVFNVDLGMTQGVSFHGLNYEYTSN
                     ARKFENFELFVNATGMGDKLIIECTYNTDLFEDEMMQLRMQEFVVLLNGLVEYADSPI
                     KNLPLLTDDEKRKLFIKWPVVKENNLRSECIHTLIEKQASAHPDANAIWFNEQTVTYA
                     QLNQRANQLAHYLIKAGVKQETLVGICHDRTDDLIVAMLAVLKAGGAYVPLDPNYPSD
                     RIAYIMSDAKAPLLITSSKYAKQLGETGTRLICLDKEVTEISIEKTAKPDVKISCDQL
                     AYIIYTSGSTGKPKGVAIEHRNAVALIDWAQTVYSSEELAGVLASTSICFDLSIFEIF
                     FTLSSGGRIVLVKDAMALNALSEKAGVTLVNTVPSAIAELLKLKGGVPSSVKTINLAG
                     EPLSVELVQKIYSQTTVHKVYDLYGPSEDTTYSTFTLRKSDGPYTIGKAITNSQVYIL
                     DKNRRPVPIGIAGELYMAGDGLARGYLYKPEMTEERFVDNPFVPGQRMYRTGDLGKFM
                     SDGNIEFLGRIDNQVKIRGFRIELGEIESMLRIRKEIKELVVMAREDQPGNKILVAYL
                     SANAEKEIDVNELRQHLRTELPEYMIPSVFMILENIPLTPNGKIDRKALPVPEVEVKV
                     ESANFEEPHNPLESLLAGIWSEVLGIDKIGIHDNFFELGGHSLLGIRMMGAIEQKTGV
                     KLDYPTLFRASTISQLAKVMNAEELHIDWPIVVPLQPNGKERPLFFVHMHNGNIQRWR
                     VLLKHINKDQPVYAIQPRGLDEKYDYHYSVETMASFYIDEIKKIQPVGPYRLAGLCFG
                     GTTAFEMARQLKAKGETAELVFMINNYAPPSNPMQYKIRETWDEFKGMTLEDKLEFAL
                     EKTKNAGKKVLSKLTKVFDSGNTATSEVKVNTKPDIRWVNSVALLNYQPESEYSEKVV
                     LIKTGEEIARHYDETLGWKRLINGEIEMHQIPGSDNDTIITHKEYYTQLAQYLNQALN
                     QIK"
     gene            44142..44852
                     /locus_tag="PKY09_03410"
     CDS             44142..44852
                     /locus_tag="PKY09_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNB11933.1"
                     /translation="MDLQITPEVVFCNDLLIQDNECLVFIFSSDSFAAVEDDLKKLLS
                     KEETERFQKFNFEKDRQTYCIAHGLKRVLLATLLNTKTHLEIAVHLSGKPFVKEQKSS
                     MVLHFSLSHSEKMIALAFQKNNPLGVDIEKIQSADKYENVIQYYFTSDEQKQIARSDS
                     ATFFKIWTQKEAIGKATGQGIANLPEITAPGEYDLSTVSLDGYVLSTCFFRGNEISFK
                     TIHAQDVLNQIKILSLRP"
     gene            44919..>46449
                     /locus_tag="PKY09_03415"
     CDS             44919..>46449
                     /locus_tag="PKY09_03415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNB11934.1"
                     /translation="MNKTSFHELSLSHEMLQAIDEMGFTEASPIQAQAIPVLMQGQDV
                     IGQAQTGTGKTAAFGIPLLEMLDPKVKATSALVMCPTRELAVQVAQEIKKLAKYKKGV
                     VVLAVYGGEQIQKQINALKRPVHVIVGTPGRIMDHLDRRTINLSKIQMVVLDEADEML
                     NMGFREDMEAILQHAPDERQTVLFSATMPKPILEITKRFQNNPKLVKVTKQELTVSSI
                     EQIYYDIPSSQKADVITQLVELHNLKLMLVFCNTKRKTDEVTEQFQQLGYKAEAIHGD
                     LRQNQRNNVLARFKKGDVNMLVATDVAARGIDVENVDAVFNFDLPLDEENYVHRIGRT
                     GRAGRSGKAFSFVSGRNDMAKIREIEDYSKVRIERHQLPSGKERIELAKIKLAARLKV
                     LIEEGDMEQYDNMFATFKQEGLTLHQLAAALLKMHFAPMLQQSQSKSSSKSSGREERF
                     GREERSGRGKRDDGGGRGKRGKHSGEADMIRLFLSVGKKDKVSKGDIVGAVSGLSNMS
                     SS"
     assembly_gap    46450..46549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <46550..46741
                     /locus_tag="PKY09_03420"
     CDS             <46550..46741
                     /locus_tag="PKY09_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNB11935.1"
                     /translation="SKGDIVGAVSGLSNMSSSNIGVIDIYDKFSFVEVNGEFLNDVLS
                     NVNGNKIKGRKVNVEIAKD"
     gene            complement(46738..47313)
                     /locus_tag="PKY09_03425"
     CDS             complement(46738..47313)
                     /locus_tag="PKY09_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNB11936.1"
                     /translation="MEFSFILNEININNQLYVVHANYPSDKESRKLFQKEINIKLLRQ
                     VTKNSTLQLHYQETGKPFIDGNHAISFSHSGNYTALIITESKSAGIDIEMTDRNIEAG
                     IDYFLNATEIAFLSQKKKNLHALMCWCIKESVIKHFNISGIDFKNCIQIQPFECSNEG
                     LVHAIVQHSQKDTISIKYRREKDFLLAYTVA"
     gene            47366..48049
                     /locus_tag="PKY09_03430"
     CDS             47366..48049
                     /locus_tag="PKY09_03430"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11937.1"
                     /translation="MTTPDVFQFGIQVTSEVATVQIGEASMTYFKVTNIDALYAALSQ
                     LDSNDDNVMDERIPYWAELWPSAIALAEYVSELENSEVSFIHEIGCGMALPSVLCGKL
                     GHKVMMSDYVEAPLQFAEANWHLNNTSQPLTMMLDWRNIPDNIEKCDLLLAADVAYEG
                     RMFPAVINAFQRLTKNGGKIIFTEPNRKFTSEFYKLLLNNFTKINSFDRLVFLNNHQH
                     KIKLSVINV"
     gene            complement(48068..>49103)
                     /locus_tag="PKY09_03435"
     CDS             complement(48068..>49103)
                     /locus_tag="PKY09_03435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11938.1"
                     /translation="ALLEKYKLETVFLDASVNAWDAFTNLFKPNESYNIVRFDTETFH
                     QKLKELLQSETFDFILFEGLFLSPYLQTVRTFSKAKCILRAHNVEWLIWKRLAKATKN
                     PLKKTYLYFLSDRLKRYENKVINNFDAIVAISELDKNLFLKDGCNIPIEVAPTGMNTA
                     KYKNIEQMDADSLSLFHLGSMDWLPNIEAVDWLLNDIWPLIQQKSNKVHLFLAGKNMN
                     PKYFNIKSENLIVAGEIKDALKFMENKQIMVVPLLSGGGIRIKILEGLAAGKVIVSTS
                     TGAEGINYTDKKNILIADTPEDFTKTILSLLNNPNLLNNIAREAQILARTQYDNILIG
                     KKLAGFLKTI"
BASE COUNT        15384 a         8641 c         9297 g        15681 t
ORIGIN      
        1 ctggaaaaag tttatgataa aatagtttca aaaggtgcag cgttagcggg tataaaaaaa
       61 gctttgtttt tctgggcatt gaacctgggc ttacgctatg agttgaaagg tgctaatgga
      121 tggtggtacg agtttcagtt gcgcttggca aacaagctta ttttcagtaa atggcgtgaa
      181 gcattgggtg gaaatattaa ggcaattgca tccggtggtg ctgcattgca accacgcctg
      241 gcacgagtat tccatgctgc aaaaattccg gttcttgaag gttacggact gacagaaaca
      301 agtcctgtaa tagcagtgaa taattttcaa cccgacagta ttaaatttgg tactgtgggg
      361 actgtacttg aaaatgttga tgtgaaaatt gctgccgatg gtgagatatt gtgcaaaggc
      421 cctaatgtga tgcttgggta ttacaacagc cctgagttga ctgctgaagt aatagacgct
      481 gaaggctggt ttcataccgg tgatattggt gtttttgaag acggaaagtt tttaaaaata
      541 actgaccgta agaaagaaat ctttaaaaca tcaggaggaa aatatattgt gccacagatg
      601 atagaaaata aattaaagga atcaagattt attgaacagg caatggtaat tggcgaaaat
      661 caaaagtttg catcagcatt tattgttcct aattttgcat ttatcagaga atgggcagca
      721 aagaaaaata ttgcggttaa ttccaataca gaaattgcag ccagtagtgt agtaaagaaa
      781 cgcattgctg aagaagtaga agtggtgaat aaaactttgg ggcattacga aacaattaag
      841 cgttttgagt tacttgatcg tgaattcagt atagagcgca atgagatgac accaaagctt
      901 agcttgcgca gaaaaattat tcttgataac tataaagaaa agttagccaa aatatacaag
      961 gatactgagt gaagtagatt ttactgatga ttacaagaaa gcagcctgtt accatgatgt
     1021 attcaaaggc tttaataaag cctgatggtg aggtactgaa ttattccgat acatcttccg
     1081 gtaatgaagt attggttttt attcatggat atccttttga tcagtcaatc tggaaagagc
     1141 aggtaatagc cttgtcagag caggctcgga taattaccta tgatgttaga ggttatggaa
     1201 gcagttcaaa agggaaagag aaattttcta ttgatttatt tgctgatgat ttaatttttt
     1261 tactggattc tttacaagta aataaggtta ttgtctgtgg cttgtccatg ggtggctaca
     1321 ttgcacttaa tttagtcaag cgatatactg atagagtcaa aggacttgtt ttgtacgata
     1381 cccagtgcta tgcagacaat gcaatagcaa aggcaaaacg atatgaaaat attgcattag
     1441 ttaaaaatgg cggaaaacaa aagtatctcg aggatatgtg taagattttg ttttctcctg
     1501 tcacatttca gcagaagtat gaaataataa atcagacatt aaaccttatg atgaaagttg
     1561 atgatgatgt gttgatgaga acattagaag caatggcaga aagagaagag tcatgcagca
     1621 tacttgcagc aattaaaatt ccggtaatga tattttgcgg tgaacatgat atgctgacgc
     1681 ctccggaaaa atcagaattt atgcaacaac aaattcaggg aagtgtttta catataatag
     1741 aaaattccgg tcacttgggt catcttgaaa ataaagaggc atttaatcag catctgaata
     1801 actttcttaa atcgttttaa gttttttgtt ttttcttttt agcggcagga aacaatacat
     1861 tgtttaaaat cagtcggtaa ccgggactgt tcggatgtaa attcaggtct gtcggagggt
     1921 cgccaaccat gtgttgataa tcttcgggat cgtgacctcc gtaaaatgtc catgtgcctt
     1981 taccaaaatc accatgtatg tatttagctt cattagcagc tttgttttcg cccatgatga
     2041 taacatcagg tttaatataa gccttgttga atgctgttgt ctgacccata aagcctttaa
     2101 tcacctgcat atgatcctgg caaagcatgg taggaaccgg atcccacttt gctgaaaagt
     2161 cgaataaggt aaacacatct tcatcgcgag gtactcttct tgttgttgtg acatcaatat
     2221 ttgaaaagcg atattcaagt ggattgacac ttaaagtata atctctaaat gcaaatgttt
     2281 ttgaaaaatc cagatgatca ttgtaattag atgatgtgcc atcgccatca tacatgcttt
     2341 cgcaaatatc caagccttct gcagagagtg caatgtcata cgtgtctgta cccgaacaca
     2401 tggtaaataa aaatccgcca cctgcaacaa actcttttat tctttttgct acggcaagtt
     2461 tcatttgcga aactttagca aatccaagtt gcttagccat actttccata cttctttgct
     2521 gttcctgata ccaggttgca tttctataca tattatagaa tttgccatac tgtccggtaa
     2581 aatcttcgtg atgtaagtgc atccagtcat atagtgctaa tttaccctga ataatttcct
     2641 catcataaat aacatcataa ggtatctctg cataggtcag tgcaagtgtc acagcatcat
     2701 cccaagggcg tttatcttta ggagaatata cgcatatttt aggtactttc tctaatttaa
     2761 caacgtcctg attaacttgt gggtctaata tttcagaaac aatctgattg gcctgacctt
     2821 ctgaaatgac attgtaacta acaccacgta attttaactc gctttcaaac ttcggattct
     2881 gaataaacat aaaacttccg cctctgtaat tcaacatcca gttaatttct attccgcttt
     2941 gcagaaccca ataggcaata ccataagctt ttaaatgatt ggcttgcgta tcatccattg
     3001 gaataagaat ctgtgcagcg ttagctcgca gatttgctat tgcaagaagt gtgattaaga
     3061 ttgatttaat ccgcataacc tttaatgttt gctattgaat aatgaatgat tgacacagcc
     3121 atttgttttc agctgaagta tttaaaaagt aaatgcccgg ttttgaattc aactttatcc
     3181 tgttgttact ttgatgtaat tctccttttg aaattatctt tccatttgca tctgttatgg
     3241 tgtaattgca atttgttttg catactacat tgataaaatc atgcacaggg tttggataaa
     3301 tttgttgctt ttcgttacta atttcttttg tttcaatacc tgttgtaaca agattgttgg
     3361 ccgacaaaat ccataaatca gggtcaaaca caaccgaatc tacctgccat gggactgccg
     3421 cagtaaactg ttgccccgaa aatgtatgat taaataccaa tgtggtgtcg gtgccattac
     3481 catagaaccg aacaggtacc ggcatttcaa aaaatgcaac tgaagggtgt gatgttgtct
     3541 gatcaatttt tagccaaact gcagggttat tctgattcca ggtaatatga taggaagggt
     3601 agccctgatt gtaataccat tggttaaaaa aagtactcag attttgtcca cttgtgtttt
     3661 gtaaatgagc tattaagtca ggtgtcgttg caaaaccata tttcaacaaa gtgtcattaa
     3721 gataattagt taatgcatta aaaaaaacag agtcgccaag ttgccagcga agcatgtgca
     3781 ataaatacga tcctttatta tacgttaagc gaccattaaa tattctgctc acatcattgg
     3841 tgtcagtaca aaacacagag ccatccggct gagaggtgat attgttaatt ttttgtcttt
     3901 tccagttata ccacaaagat gggatataat gttccttggt taatccttca aaatatgttg
     3961 caaaaccttc gtttagccaa atatcttccc aactaccgca agttattaaa tcaccaaacc
     4021 actgatgtgc acattcatgt gccatcaggt cctgattaaa attaaccata aaactcattg
     4081 tttgatgttc catgccaccg ccccagttaa attgagcatg cccatacttt tcattcctga
     4141 atggatatgg aatagtgagg ctgtcataaa gttgaatgat aggaatgata tccggtgttt
     4201 gcaattgtgc tgacgataag ttttcaggat aaacataatt gagtacttgc aaagaatctc
     4261 caactgccat aggcacatag tcggaataag cagcatagtt agtaactgca atggcaataa
     4321 gataagatgc agtagggtag ttgcttttcc agtgtgtgat tttttgattg cctgaagaaa
     4381 tttcagaaat taattttcca ttggatgcaa ctttatatgc tgtgtcagta gttacaaaaa
     4441 catccatgct atctacttta tctgtaagcg agtttttgca tggccaccaa tcgctggcgc
     4501 caaaaggctc tgaaagcgtc cacaataccg ggattccatt atgcgtactc tgattaaatg
     4561 atccaaaacc tgaagtgggt ggtacaccat gataaaaaat ttctacgcta tctaattgcc
     4621 ccgttgagat tatgccggga agtaaaattg ttataatatc actactgctt gtaaagggga
     4681 gtaaagttga ctgatagaga atactatcta ttgtaagagc tgaagaaaaa tcaaactcca
     4741 atttgctaag cgatgatttt acttcaaata atgtaaatac atttccggaa atatatactg
     4801 agtcaggatt tattacccag ttaaaacgat tgtattttac atcaaaatcg ctacctgttg
     4861 tgctaacttt gtttaagcta tgtagttttg aattgtctga aatgatacgt ttgcaatgaa
     4921 catgcacatt atcctgactg aaacctgaaa atggagataa aataaaaaat aagagtaatt
     4981 ttctcatcat ttatcggcat acttgtattc acgaagtaac aagtcaattt cagttatcaa
     5041 tctgttgaca tcaatagaat cagtgccatc ataataacct ctgattcgtt tttctttatc
     5101 aattaacgca aagttttgcg tgtgcacaaa atcatccggg ccaccatcac cttttgttgc
     5161 agttactaaa taggaaatgc gtgcaaggtc ataaatcttt tgtttgtcac ctgtcacaaa
     5221 atgccatttc ttagcatctg catgatgttt atctgcatat tcttttaaaa ccgcaggtgt
     5281 atcatattcc ggatttactg aataggacaa aaacattacg tcaggatttg atttgtaatg
     5341 ctcaaaaaca cgttgcattt gggtggtcat aacagggcag atacttcggc acgtggtgaa
     5401 gaaaaaatca gtcacataaa ctttattgga ataatccttg tctgtaaccg tatccccatt
     5461 ctgatcaata aaagaaaaag gtcctatttt atgattggat ttgttttgtt catcctgcat
     5521 tccataaaca ggcaatgttc gtaatggtct gtcttcatta cacgatgtcc acaataatcc
     5581 taaagttata gttattaaaa taatgagcca ttccaaagca gggattgacc aaagtttttt
     5641 acaacttatg cagctcattt ttcttcaaga taggtgagag gttctttaag aaaatcatcc
     5701 ttacatgccg aagcgcaaaa accataaatt ttgccatcat acattgctgt atcggaaaac
     5761 tcttctttaa cagacatttt acaaaccgga tcaatatctg atgctaatgc aatatcaggt
     5821 tttgccggaa attctttttc ttcaagggcc ggtgctgtct tctgttcagg ctctgaagtc
     5881 ttaggttgac aggccgtaaa aagaactaat agtgctacaa taactttcct catttttttg
     5941 tttatttgaa ctttacaaag ttattaaatc gaacctttat cagatgaagg attcaggtat
     6001 tattttaaac gaatcaattc aaaaaatatt atgataaaac aagtttacac tgtgctgacg
     6061 atacttatga cttttggaat gattaatcag gtgaatgcgc aagaaaaagt tgccatttac
     6121 catcctgaaa agaatacatt tgaacagata aagcaagctg aaattactgc acagaaagaa
     6181 gggaaacatg tttttctgat gattggtggt aattggtgca gatggtgtcg tatgtttgat
     6241 gccttcagca agacagattt gtcggttgac tcagcattta aggcaaatta tgtaattgaa
     6301 catattaatt acagcaagga aaataaaaac gattcattga tgcaacaact ggaatttccg
     6361 caacgatttg gatttcctgt gtttgtaatt cttgatgctc agggaaaaag actacataca
     6421 caatctaccg gctatcttga gcaaggtgag gggtataata aagaaagtgt tattgaattt
     6481 ttaaatcaat ggtcacctgc tgcattgaat ccggcaaact acaagaaata gctagaaaga
     6541 ttaataagaa ggatttttta tagaagcacc tttttgattg taaaataagg ccatctgtct
     6601 attaccctga attttataaa tgcggctcaa agagtcgtat gcagttacgc agttaggata
     6661 gtgctgaatg tttaatttca gaatcttaat tgcatcatcc aaatggcgtt tacgaatgag
     6721 ttgttttcca atagctaagt actgtgtcat actcagatta gtttcttctt catttgtttt
     6781 tttaacctta tatgttgcaa gttctttctg aatacttgtc atccgggaaa taaagaaatc
     6841 agccgatatt gttccgtcca ttaaatcttc gctataaagt tcaagacttt gcttaagctt
     6901 gtttattcct gctatgcccg gagttttatt ttttttataa tcggccatca ttttttcccc
     6961 ttcaactaac cacaatgaat ctttatttga ctgaagaaaa cgctcaaaaa tcacaatgtt
     7021 ggcaagtgca atctgagagt agttttgtga ttcattttta aatccaccac attctgtttt
     7081 ccagatctgt gtaaacaatt ctatgccacc ttttttaggg ttaagctcat taatcacaca
     7141 ggctacattt cccggtcctg catggtaggc atgcattaca agcaggcgga accataaatc
     7201 atcttcgtta aacggaatgt ttttttcaat cagcaagcct tttaccttag ggatacatga
     7261 actttttaag agttgcgatg cagcatatgc agatttatta aggtcagttc tttcgtcaac
     7321 atgcttgtta acttttaaac caaattttat agcaacactt ttcatcagtt gaaaggggcc
     7381 attggctcca acataagatt tgttgtgatt ttttccggga ttctcaatca gtaaaattgt
     7441 ttgtgcatac caagggtcaa cattattatt tttaaaaatc tcaacggatt ttccgattac
     7501 aggaataacc cttttgtatt cataaaactc tttttttccg aatgtaacgt aaagtgtagt
     7561 gttggtatca agaccgtttg ctaaattgat aaatttctta taggctgttt tttctgattc
     7621 agactgacat ttccaatctt caaatggagt tttatgtaaa atacatcgtg tagatgcaac
     7681 attaatcagt gccgtatcag cagactgtct gattacttcc tgccaaaatt taacctgttg
     7741 aagcgtgtcc catctctcag caaaaacatc tgtgttggaa acaaaactca aacgtgttgt
     7801 atcatcgtaa ctgcaaacaa ctctttttaa taaaggtgta tcagcactta attgcagacc
     7861 gatgaacaga agtaaaagag aaagtacgat ttttctaaac attatttgca tttacagtga
     7921 tacgtaaaca gacataacct gttttgactt tttttattgt tttcttgcat cttgcaaagg
     7981 tatgctattt taaattgaaa ttttaagcca atgcaagtta agtaaaattg gttttcaaca
     8041 caagataatt aacttttaat agaagtgtaa ttacaatatt gcaattcatt tgtcacatta
     8101 aatgcaaagg gagattcatt ttcagaatcc cccttcgcta taaaccaaac ctatgaaaaa
     8161 ctatctataa tccaaatcca taataagctc tcatgccatt gggataaaca ccttctataa
     8221 gtacaccacc ttttttgttg gctaatgctg attcaatatc gtccggagag ttgattttct
     8281 tattgtcaat actggtaatt ataaatcctt cacgtatgcc tgcacttctc aatttaccgg
     8341 attgcaagcc agcaaccttt acaccattac ttatgcctaa ttgttttttc tctttatcgg
     8401 tcacttctcc aaattcagca cccatcatgt tgacattgtt gtttgcacgt tcaacacgtt
     8461 caccaccact gttatcacga agtacaatct gatagttttt ttcagagcca tcacgattca
     8521 atgtaagatt aactttgtca ccgggaccga aacgaccaat ctgttcctga agttcgcttg
     8581 cagaattaac tgtagtctca ccaacttttg ttacgacatc acctggtttt atacctgccg
     8641 cttgtccggc accaccactc acaacgccat ctatatatac accctgcata gacttcaaac
     8701 ctttggtctg agccaattgc tggtcaatat ctcttaatgt aacaccaata aatcctcgtt
     8761 gaaccttgcc atattccaat aagtctttag ttacttttcg tacaatattt gcaggaatgg
     8821 caaatgagta gccggtataa gagccggtgt tggaggcaat agcagcatta atacctacca
     8881 aatcaccatt caggtttacc aatgcaccac cgctgttgcc gggattcaca gctgcatcag
     8941 tttgtataaa tgactcaatc ggaaactgct gatcgtttaa gatatgaatg ttacgacctt
     9001 ttgctgaaac gatacctgca gttgcagttg aagtaagatt aaatgggtta ccaacagcca
     9061 acacccattg tcctacttta atatcgtctg aatttgcaaa ttttgaatag ggtaaacctg
     9121 tctggtcaac cttaagcaat gctaaatctg ttgaaggatc ctttccgata actttggcag
     9181 tgaaagttct tttatcatct aaagtaacat caactttgtc tgcattgtca acaacgtgat
     9241 tgttcgttac aatatatcca tcatcagaaa taattacacc tgaacctgct gattgttgtg
     9301 gttgttgtgg tgcgccatag ccgggtgctc cccaaaagtt aaatggatta aaaaaattac
     9361 cactaactgg ttgtgatgga taagttgttt taacgtgtac aacagcagga actgtttttt
     9421 ctgcagcttc tgtaaaatca aacatggcag tttctgtgtt gcctgttatg cttacccttt
     9481 taacgggcac tacgttttcc tgttggccaa acaaaacagg tttttcagtt tgtttataaa
     9541 tggccaatga agtaaagccg cctattgctg caattgcaag tattccggct gtttttttaa
     9601 tctgtctgat cattatctgt aatcttttat gttttatttt ctaatttgac aacgaagtta
     9661 tgttacattc agtgaaatga ctatgaagtg aacgttaaca atcattaaca atgtttcaaa
     9721 ataatcacgt agttttacaa cgtttaatat taagatttag ttttttaaag aataaaaatg
     9781 catctaaaat tcagcaagta tcagggcgca ggcaatgatt ttattttgat tgataatagg
     9841 tcaagtaagt ttagtacgct gaaaccaaaa cagattgcca tattatgcga caggcatttt
     9901 ggtattggtg cagacggact tattttaatt cagcatagca gagatgccga ttttgagatg
     9961 gtttattata acgcagatgg taacattgga agtatgtgcg gcaatggtgc acgatgtgca
    10021 gttttgtttg gaaaacaaat tggtgttgca ggtaaaaaga ctcaattcat ggcttatgat
    10081 ggtttgcaca ctgctaaaat caatgcggta aacaattttg ccagtgcaat tgtgagtgtt
    10141 tcaatgtccg atgtatcgga gataaaaaag cataatggaa attttgtgct gaataccggt
    10201 tctccacatt atgtaaaatt tgttgatgat gtagagaaaa ttgatattgt taaagaagga
    10261 agaagaataa gaaacaacaa tacttttaag gcaaaaggta ttaatgtgaa ttttgtagca
    10321 tataaaagca aagcatcaaa catcagaact tatgaacgtg gcgtagaaga tgaaacactt
    10381 gcctgtggta ccggtattac tgcatcagct attgctgcac attattctgg tttaactaaa
    10441 agcacttctg tgaatatgat tgcgcgtggc ggaagtctga atgttacatt cactgtcaag
    10501 gataatcagt acaataaaat tgtcttgaca ggctctgcta tgaaagtttt tgatggtgaa
    10561 attaaaatct gaatttagca gatatcatgg tatgtcttcc tgcctgtgga tagtagaagt
    10621 tttcgttaac cctcatgcca tcatatagat agccataagt atagccattg cttgaataca
    10681 tttcattgaa caggttaaat acagaaactc ctgcgtcagt ttcaatgttt ttaaaaatct
    10741 taaaagtata atgtgctgaa atgttggtgt aggcaaatga cttcagtgat ttttcttcgg
    10801 aagacgtgtt atctaaatac tgttttccaa catacttctc ggtgagttta atgctaagct
    10861 tttttaaggg ttttatttca acacctgctg cagcaattat cgaaggagat aaggcaatgt
    10921 gggttttctt atagatgatt tcctgctgcc ctatataatt aaaatctgca tcgtactgat
    10981 caacatattc cttaaagttc agaattttgt tttcacttaa tgttacattg cctgaaacaa
    11041 ctaaaaactt gctcgcatca taattgaatt ctgcctctaa gcctcttcta aaactatttt
    11101 ttacgttttg tcttgtataa ttgccaacgt cattaatttt acctgttaaa atcagttgat
    11161 ctttatattg catataaaag gcattaaaac ttaattttaa tttttttatt tcaattgaat
    11221 aacctgcttc aaagtctatg agattttcag gtttaggtcg tgattttgca gttgactcgg
    11281 taaaatcatc tcttgatggt tctttgtttc caacagcaat tgacagccat ccactttcgt
    11341 tatcatttct taaatatgta acaccggctt ttggatttaa aaaatgatac gctgcaatct
    11401 gattggcatt tgttgcaaag tcatcaaatc cggtaaaccg ataatgtacg tagcgatatt
    11461 gcatgtcggc ataagcaatg aaatgttcac ccaggtgtaa agttcctttt agccaatgat
    11521 taaaattatt ctttaagcca ttgttttgat agtaagtgta attttcagag gctaatggaa
    11581 taaaatcacc tttaattatt ttaccatagt gattaccatc atactgattg ataccaccac
    11641 ctgtttttat ttcaatgttt ttattctcat agttcagtga gtaaatcatt ccataaaaat
    11701 aattgtcgag ccactttctt ctaacaagat ttgtatttgt aattaatgta tcctggattt
    11761 gaataggagg tagcccatag tcaccaaact ttgcgttttt tttatattcc tcatagtagc
    11821 cttttccgcg tgtcatgtgc gctgcaacat ttaatgtaaa caagttgttt atctgatgtg
    11881 agaagtgcaa ctgataattg tcctgttggt agttgtctgt ttgccccgga tataaatagt
    11941 aattgtattt tcttcctgat tcaagaaggt tggcagcatc ttcgttatct aagccgttac
    12001 gtgaaatata gtccatcatt ccctgagtat cgtttttata tcttgcttcc ggcacaccat
    12061 accaggattg ataagtcttt tctatccctg aaaacattat tgccttcaac attgactttt
    12121 gaccaaagta tccgcctgaa aaataaaaag attttaaatc agaggtcgct ctgtcaacat
    12181 atccgtcaga ctgtatggaa gatagtctgg tttcaaatgc aaaatggtta ttaagtaatc
    12241 ctgtaccggc aataagattt accttttttg tattgaaggt cccgtaactc aagttggttt
    12301 ctgcaaatgg tttttctgac agatgatctg taagaatatt aatacttgcg ccaaatgcag
    12361 ctgcgccatt tactgaagta cctacacctc tctgaagttg tatctgattg gttgagctaa
    12421 gcaagtcagg catgtcaacc caatacatgc tttgcgattc ggcatcattt attggaatac
    12481 catttatagt aacattaatt cttgtggcat cactacctcg cacacgcata cctgtgtaac
    12541 caacaccggc acctgcatca ctgcttatta cgacacctgt aagttgatcc atgatttcag
    12601 gtacatcttt ccctgaatta atttcttcta tttcattttt attcagattg ttttgtgcaa
    12661 tgggtatttt atttttcatt tttaatgatt gtataattac ctcatcagtt acaaaagctt
    12721 ttttgaaaag tacaacttct acatgttggt tacttgtcag gttaatttca ctgctgtatt
    12781 cgtggtaacc aagctgatat acttgcaaag tgtatttgcc agccttaaga ttaaaaaagg
    12841 taaaccttcc tttttcatct gaaaaatgtc gagtcagtga ttctttaaga gtaataactg
    12901 cattatctac ttttttacca tcattagtac tcacaactcc tgacaggtca aactgtgcat
    12961 tagaataaag gctaaagcat aaagccaaaa gcaacagaga aaatttctta atcatctttt
    13021 tgtttattta ataagtaaca tcacaaaaaa agtagcagtg caataaaaga atgttaccta
    13081 ttcctacgca ggcattatcc tgatcaggtt atgggtataa tctcagcatt ccttttatta
    13141 caggaagcac cccttttgat gacacaaagt aatcaattat ttgccgttat tacaaaatat
    13201 cctgtaatga cattttttag cttgttgaaa tctttttgaa caaatagtgt attttggttg
    13261 aatgtttttt tggattaatg atttttttac tattaacttt gtaagataaa ccgaaacaat
    13321 ttatgaggca gctgcgcatt caaaagtcca taacaaacag agagagtcaa tcgttggaaa
    13381 aataccttca ggaaataagc cgcgaaggca tgattactgc agatgaggaa gctaatttgg
    13441 cgcgaagaat tcgtgagggc gatgaggagg ctttgagtaa actaacaaga gctaatttaa
    13501 gatttgttgt ttctgttgcc aaacaatacc agaatcaggg attaagtctt cctgatttga
    13561 taaacgaagg taatctcgga ctaattaaag ctgcatcacg ttttgatgaa accagaggat
    13621 ttaaatttat ttcttatgca gtatggtgga ttcgtcaaag tattttgcag gctattgcag
    13681 agcaggcacg catcgtcaga cttcctttaa atcaggtagg ttcgttaaga aaaataaata
    13741 aagttttttc aaaattagaa caagagtatg agcgtgagcc ttctgctgag gaagtagctg
    13801 aaataatgga agtgcctatc gaaaaaattt cagacacatt aaaatcaccg ggcaagcatg
    13861 tcagcatgga tgcgccattt gcaggcaatg aagaagccac cttgcttgat gttatgcaaa
    13921 acaatgatgc accggataca gattatggcc tcatgaatga gtcacttcga aaagaaatag
    13981 atcgttcttt aaaaacattg actgagcgtg aacgcgatgt agtcaaatta ttttatggta
    14041 ttggtgtttc gcatagtttt tcattagacg aaattggtgc aaagtttggt ttgacaagag
    14101 aacgtgtaag gcaaataaaa gagaaagcca taagaagatt gcgttcaggc tctcgaaata
    14161 aattgcttaa agattattta gcctgattat aatctaaaga ggcaatgttt taccttcgcc
    14221 acatcaacaa agaatggcgc aaaagaaggt aataatttca gtaattaatg acatagcaac
    14281 cgatcaacgg gttaggagaa cagcatcggt attttctgag ctcgattatc aggttcttat
    14341 tgttggacga aagttcccat atagtttacc tgtttatgag atgcccggtt tgtcggtaaa
    14401 gcgatttagg ttgcttataa acacaggccc tctgttttat gcattatttc agataagact
    14461 ttttcttttt ttatttttta agaaaacgga tatactatat tcaaatgact tagatacact
    14521 cttgccgaat tatttagtgt ctcgactgaa aggatgtgct ttgatatacg acagccatga
    14581 gtattttaca ggagttcctg aattgcagaa taatcatttt gcacgcaaag tatggacaaa
    14641 ggttgaggct tttattttcc caaaacttaa aaatgtaata acagttaatc aatcaattgc
    14701 aaatctatat catcaacagt atggtattga tgtgaaagtt gttcgtaatg ttcctgataa
    14761 aacagatagc attaattttg atgtaatgca atggaaaaaa tctgttgggc tttctaatga
    14821 taaaaaggtt tttattttgc aaggcgcagg aatcaatatt gacagaggag cagaagaggc
    14881 aattgaagca atgaaaaggg ttgatgctgt gttacttatt ataggtggtg gagatgtttt
    14941 tcatcggttg caagaattgg ctatcaaaca aaaagtagat caaaaagtaa ttattcaccc
    15001 taaagttgca cgtgaggaat tgattgccat aacgtcatca tgctttgcag ggcttacatt
    15061 ggataaagac accaacatca attataggtt tagtttgcca aacaaattat tcgactatat
    15121 acatgcaggg attcctgtta tagcttcaga cttgcctgag gttgcagcaa ttgtaaacga
    15181 atacaaaacg gggattgtgt taccgaagta cactacacaa gctatagctg aagctatgaa
    15241 tcgcttaata attgagaaag aactttacat taactataaa gaaaattgta gtattgcagc
    15301 tacggattta tgttggcaaa atgagaagaa gatattgatt gaggtaattt tatgttctga
    15361 gaaaaagaca aataaacttt aaaacagata gtgagaaaca gcggtaagca cttacacatt
    15421 attagttttg acataccata tcctgcaaat tatggtggag tcattgatgt gttttataag
    15481 ctccgcgctc tcgtatctgc cggagtaaaa gttcaccttc atgcatttca gtatggccgt
    15541 cagtcatcta aaattctgga agaactgtgc gagtcagttc attactatca gcgagatgtt
    15601 tccaagtcac agttgttttt aaaggagcct tatattgttg tgtcacgatc gtcagagcaa
    15661 ctgattaaaa acctgctgtc agatgatcat ccaatactct ttgaaggatt gcacagtact
    15721 cattatttga acgatgaacg actaaaaaag cgaaagaaaa ttgtcaggac gcacaatata
    15781 gaacatgaat attataaaaa cttagcaaaa gcagaatcaa acatctttaa gcgatactat
    15841 ttttataatg agtcaagtaa gttggaaatc tacgaaagca ttttattgaa tgcagatatt
    15901 attgcagcaa tttctcctaa cgacaccaaa tattttaata gtaagtatgg taatgcacat
    15961 tacataccgg catttcattc ctttacaaaa gtaaattcga gagttggcaa gggagcatat
    16021 gcattttatc atggtaatct ggctattggt gagaatgata tggctgcgct atatttagta
    16081 caggaagttt ttaaaaactc accggttacc ttactcattg caggcagcaa acccggtgtt
    16141 aatttaagag atgctgtgca acgaaagaat aatgttactt tattaagtga tttgaatact
    16201 aagcagatta acgatctggt tttagacgca catattaatg tgctacccac ttttcagcca
    16261 actgggataa agctaaaact tctttctgcg ctttttagtg gtcgttttgc actcgtaaat
    16321 acaccaatgg ttcaaaatac cggattggag caactttgca ctgtagcaga cacacctgaa
    16381 gcaatgcgtg aagcacttgc agccatgatt aagcaggaat ttactcagat agatatttcg
    16441 ctacgggaag aaattcttat ggaaaacttc aataataaag cgaatgctct gaaactaaaa
    16501 gatttgattt tttaaattta tttttacttt tgaaaaaaaa ttcaaaatgc ttcaggtaac
    16561 aggaatatta aaacagaaat ttgcagaaca ggtagtaaca gacaagtttc gcaaaagaga
    16621 atttacactt acaatagatg ctaaatcatc ataccctcaa attgtcattt ttcaactggc
    16681 aaatgacaaa tgcaggttga tagagagttt tcaacccgga gatgaactat cggtttcttt
    16741 taatattaag ggcagagagt ggaaatctcc ttccggagag attaagtatt ttacatcact
    16801 tgatgcatgg aaaattgaaa gaacgtctgc aggagaggca ccaatggagt caccaatgcc
    16861 atctatgtcc gatttgccac aagaagatta taaggacgat ttgccatttt aaccggttat
    16921 atttattgaa tcataaaatc acaattgaat tctaattgta aattttcaaa caatgaaaaa
    16981 tgattttaag aatgaaaaat ggtcgcttgt aaagccacca tttagattta ccaataattt
    17041 gaaaattgaa gtgtcaaatt ttggaagagt cagaacttca actaaaattg ctgaaaacag
    17101 agtgctgaaa ggtactctgg taaatggata tccgattgtg aggtttaagc ttttcaagaa
    17161 aagatctgat gaggcacaaa agcaattgga tcagaaacga agaaaaattg aacaactgac
    17221 aggtaaactt cgcgatgtaa aatcagaatt gaaatctcat aaaaaaaatt cacctgccta
    17281 taacagtcat aaaaagaaaa ttgatcttgt tactgcagaa ctgaatggaa taaaggaagt
    17341 gtatagaaag gcattcagaa aggatgaatt ggagcgaaca attaactatg cacctttggt
    17401 acatcgcctt gttgcagata acttcgtaaa acaatcatct aagaaacatg atattgttgc
    17461 ccatattgat tataacaaaa gcaacaatca ttattcaaat ctgaagtgga tgactcagga
    17521 agataatatt gaacatcaaa agaaaaatcc tgtggttata aaagagaaga aaaaacgcat
    17581 tggcaagcgt ccgaaaaatg catcgggcta taaactcaat gagaagaaag tagctgaaat
    17641 aaagaagcgg attcataaag gtgaggcatt atcaaaaata tctgaccgtt ttcacgtttc
    17701 ggaaacgcaa ctacttcgaa ttaagagagg gataaactgg ggcgatgtaa aaatatcccg
    17761 ctaagtttat tcatcagcat gatttttttg ttgtaacttg atttaatgca acatatattt
    17821 tttttactct ctaaagtttt agatttttta ttatcaccac ttagttggat tgtaattttg
    17881 ttgctgattt caataattac aaagaataaa aaacgaagcc gaaaatgttt gtattgggca
    17941 ataggggcat tgctgttttt tagtaatccg tttgtgttga atttagtgat gcatgcatgg
    18001 gaagttcagt cagttcctat ggattccatt cataagcagt acgatgctgt aatcatgatg
    18061 ggtggtgcaa ttcgttatgt aaacggacag atgcaacgac cggtttttgg ttcaggtgcc
    18121 gatagatatg ttcaaaccat ggaactattt tataggaata aagcaaggca cataattatt
    18181 tccagtggtt cgggaagttt agtttataca gatgttaaag aggcagattt actgcgtgat
    18241 cagatgatac gaattggtat tcctgcagat aaactgattg cagaaaatca gtcaagaaat
    18301 acttatgaga atgcaaagaa tacagcagag attattcaaa agtcagactt taagccacct
    18361 tttttattag tgacatctgc atttcatatg cgaaggtcaa tgttgtgttt tcaaaaacag
    18421 ggaattgatg taatcccctt tgcagttgac caacattccg gtcagataat gtttacacca
    18481 gacaaactcc ttttgccatc accggaagta ctgcttgatt gggacttgct atttcatgaa
    18541 tggtttggaa tagcagttta taaagtaatg gggtatttgt aagcagataa tttattctct
    18601 gtccagaata cggactgcta ctttagaacc aaatgtattg gaactgtaac ataaatattc
    18661 aaaactaatt ttattggctt ctgtaaattc tttaaatact ttgtattcat gttgctgcca
    18721 atgcgggtag ttaaagtatt catcaaaaag aataatagta tcttttataa tttgtttatt
    18781 taatgcttcg aagactgttt tagtagaaga atacaggtca caatcaatat gtagaaatgc
    18841 aagtggggct ttgtatttct ccgtaaagtc aggcagtgtt tcattaaacc aacctttgtg
    18901 aacaacaaca ttgggttcaa agtgtggtag tttttccaaa gcaaaatgcc cttctttatg
    18961 agtggtgagc catgtttcag gcaacccttc aaatgagtca aatgcatgaa cttcttgctt
    19021 ggttacttta gcaatgtggt taaccgaagc acctttataa actccgaact cacaatatag
    19081 tccactaatt tttacttgct ttaaagagaa atctaaggca tcttgtttat ttttaaacat
    19141 tggaagatga gacatgtttt tctcaataaa ctcacaagtt gattgtaatg ctcttattct
    19201 taattgagtt tcaatgtcaa aagcatagtg agatttgaaa tacttatttt gtgcattaac
    19261 ataattttga cttttaaaat atttatccaa aattttaaaa atcatacgta gcaacatctt
    19321 cttcatgaaa cgcttcgttg ttataaagta ttaatgtaag tgaagtctaa tgtataatca
    19381 agacccaatc agatgacgca aattatggat ttgtgtgtcc cataaacgct gtgtttcttc
    19441 aatatcatcc tctactgcaa agtctgatac catgagcgat acatctgaag tcaattcatc
    19501 ggtcgtaatt tcaaattcaa taaaagcatc atcgctgtca tcaatccatt taaagcgcac
    19561 catttgattt tctcttttgt gcgtcatctt ggcttgttgc tcatcaccat cccaatggaa
    19621 agtgtaaatt ccttcacgaa tagttacatc atctgcaaac cattctgata atcctgatgg
    19681 ggtagataaa aatccgtata atattttggg agaagaccgc attggaaact ccatttttac
    19741 tttttgcttt ttctgcactg ccttcattgt acgttattgt ttaattttta caataataat
    19801 aaaaataaat atacaatcct aacttatttt attatgccat ttctgctttc ttactcatgc
    19861 gcttacgctc attttcatcg agataaattt tacgcatacg gatattctgc ggagtaactt
    19921 caagatattc atccttctca atatactcca tagcttcctc taaagagaat tttattttcg
    19981 gggtaatgcg cacattgtca tcactacctg atgctctgat attggttaat ttttttcctt
    20041 cacatacgtt caccaccaaa tcgcccgggc ggatatgttc accaataatc tgaccaccat
    20101 aaactgtttc gcctggatca ataaaaaatg ctccacgctc ttgtaattta tcaatcgagt
    20161 atgcagttga actacctgct tctttagcaa gtaatacacc catgttcctt cccggtattg
    20221 caccttttac aggctcataa gaattaaaac gatgtgccat tatagcttca ccggcagtgg
    20281 cagtaagtac tgcattacga agtccgatta atcctcttgc aggaatatta aactcaagtc
    20341 tttgtaaatc tccttttggc aacatactgg taagttcgcc tttacgctgc gtggcaagtt
    20401 caatcacttt gcctgctact tctgttggtg tatcaacaac taagtgttct acaggctcgc
    20461 aacgaatgcc atctatttct ttatataaaa cctgtggctg tcctaactga agttcataac
    20521 cttctctgcg catggtttca acaagaatag aaatgtgaag aattccacga ccataaacaa
    20581 gatatgcatc cggtgagtcg gtttcttcaa cacgcatggc cagattcttt tctgtttctt
    20641 tataaagtcg atcacgaagg tgacgagagg tgacaaactt tccttcttta ccaaacagcg
    20701 gactgttgtt gatggtaaaa agcatactca tggttggttc atcaactgca attgatttta
    20761 atgcttcagg actttcaaaa tctgcaatgg tatcaccgat ttcaaaatca tctacaccta
    20821 caattgctgc aatgtcgcca ctgcttacac tgtcaacttt ttttctgcct aatccttcaa
    20881 aaacaaacag ttcttttatt ctggattttt ttgcactgcc atcacgtttc atcaatgtaa
    20941 caggcatatt ttctttcaat gtccctctca caattcggcc aattgcaata cgaccaacat
    21001 agttgctgta atctaatgat gtgataagca tctgtggtgt accttcaagt gattttgcag
    21061 gaggggtctt ttcaattatg gcatcaagca aaggtgtaat gtcagttgtt tctgtgtgcc
    21121 agtcggtgct catccagcca tgcttactgc taccatataa tgttggaaag tcaagctgct
    21181 cttctgttgc ttcaaggtga aaaaacaaat caaaaaccga ttcatgtact tcgtcagggc
    21241 gacaattctc tttatcaact ttattgatta caacaattgg tttcaatcct aatgccaatg
    21301 ctttctgcaa tacaaaacgt gtctgtggca ttggaccttc aaaagcatct actaacaaaa
    21361 ttacaccatc agccatagta agcacgcgtt ctacttcacc accaaaatct gcgtgacccg
    21421 gtgtgtcaat aatatttatc ttaacgtctt tatactgaac agatacattt tttgcaacaa
    21481 tggtaatccc tctctcacgt tccagatcat tattatcgag aatcaattct cctgtttgct
    21541 gattttgcct gaaaacctgt gtctgatgaa gtattttatc aactaatgtt gttttaccat
    21601 ggtcaacgtg ggcaataatg gccacatttc tgatattttg catgaaattt ttatttgggg
    21661 tgcaaagata agacttttta acccccaaat attgattctc aataataaaa gcagaaaaaa
    21721 tcaactattt ttttgtggtg taagccctag ttcaataccc tttttaagca ttaactcaaa
    21781 tgcggcctgt ggttcattag ggcactgacc atctaaaata gcttcacgga caaaatcttt
    21841 tataatacca acattaggtc cgggagccaa accaaaggta tccataatca attgtcctgt
    21901 aacgggtggt tgccagtttc ttattctgtc tttctcttca acttctttta gcttttgaac
    21961 aaccagttca aagttgttac ggtatttggc tttcttatat tcatttttag tagtaacatc
    22021 agcctggcac aacaacatca agtcgtcaat atcatcaccg gcatcaaata ataatctgcg
    22081 tactgctgaa tcggttacaa tttcctgagc aattacaata ggcctcagat gtaataaaac
    22141 caacttctga acatatttca ttttttcatt gagcggtaat tttaactctc tgaaaatggg
    22201 aggaaccata cgtgagcctt tgtcttcatg cccatgaaaa gtccagccat gcccggcttc
    22261 aaaacgtttt gttgcgggtt tggcaatgtc gtgaagaata gcagcccatc gtagccataa
    22321 attatcagtg acctttgcaa tgttgtccaa aacttccaac gtgtgataaa agttgtcttt
    22381 atgtcctttg ccttcaaaat aatcaactcc tttcaggttg taaagttgtg gaaatattag
    22441 ctccagcaat cctgtattca tcaacaattt gaatccggtt gatggtagtg gtgaagcaat
    22501 aattttattc agttcatcgg tgatgcgctc tttggaaacg atattgattc tatttcgttc
    22561 actatgaatg gctgcaagag atttttcttc aatgctgaag ttgagttgtg ttgcaaatcg
    22621 aatagcacgc atcatacgaa gcggatcatc gctgtaagtt gttacagggt cggcaggtgt
    22681 gcgaattatt ttatcgttta gatcctgcat tccgttaaac gggtcaatca gatttccgta
    22741 atcatctctg ttaagactga aagcaagggt gttgatagta aaatcacgtc ttagttggtc
    22801 gtcttctaat gtgcctggtg taacctttgg ttttcgcgaa tgttgattgt aggactcttt
    22861 tcttgcgcct acaaactcta tttcaaatcc ttcagatttt accattgcag taccgaagtt
    22921 tttaaaaaag gcaaattctc cgtcaagcgt tacagcaagt tctttagcta cggcaatgcc
    22981 gtcaccgtta actacaatgt caatatcttt tgaagatctt tttaaaaaac agtcacgcac
    23041 aaatcctcca atgacaaaag cttttaaatt atgcttttca caaagtgaag caatctgccg
    23101 gaacaaacta tgatccagca tttctgtttg cagattattt tctgataact ttaacttcgc
    23161 cattgtttaa aatcctgatg atggatgatg gtgtatgttt ggcagtttct tgctgtcgga
    23221 actttactac atagtccact ccggtaagta tggaatcttc aatgtcatta aaaacagcag
    23281 gggagggcat gttgctcagg tttgcagagg tggaaatgat tggcttacca aatttatgaa
    23341 tcagcatttt gcaaaatgga tcttttacca atctgatggc tgcagtgcca tcatctgcaa
    23401 ttgctttttc tgagatgcga ctgatttttt cataaattat tgttactggc ttttcggagt
    23461 attcaatgat atcccatgca acagcaggaa catgctgcac acagcgattc aacatagcct
    23521 catcacttac cagagtaatc agattttttg actcactacg gtttttaagt gtaaaaattt
    23581 tttctactgc tgcatcattt gtagcatcgc atccaattcc ccaaattgtg tcagtaggat
    23641 aaacaataag tccaccttgt cttaatgttt caagacatga tttaatgtca tcactaaaaa
    23701 tttcggagtt tgaatttttc atcacactgc aaagaaaaca gattgaatcg aatttatact
    23761 atttaattat tgagctaaag atttcttaga aatcagttgt gattgcggat tccatcaagc
    23821 actttttcca ttttagaacc gcgactaccc ttaactaaaa ttgtactttc attaatagca
    23881 tgattttgta agtaattaca agcatcttct gatgttttaa atcgcaatgc atgatcatta
    23941 agtgttgtat gcaggaaatt ttcacctact aaaatgagta aatctttttc catactactc
    24001 aataagtcaa caatgtgttg gtgttctgca tttgcatcct gaccaagttc aagcatctct
    24061 ccaagaatga ttactttgtt gccatcataa ttttctgaaa gattttttaa tgctgcttcc
    24121 atacttgatg gattggcatt atatgcatca atgattattg tattacttcc ggaaatctct
    24181 atttgcgaac gttgattttc ggggatgtaa ccttcaattg ctgacttaat tttttcaggt
    24241 gtaacattaa aatatctgcc aatacatact gccgctacaa gattttcaag attgtagtca
    24301 ccggtaaggt gcgttgttat ttcatcaatc ctattgtgat tttcgaacca attaaacttg
    24361 acaacagatg ctggtgcaga aatttcgcca tagatgcata catccggttg cgtactgtag
    24421 aaaatcacct tttgataatg attgagattg ttcgttagca attcattgtt cctacgacaa
    24481 aaaattacgc catcattttc catcaggtgt ctgtacaatt ctgtttttcc tttaattact
    24541 ccatcaatgc ctccaaagcc ttcgagatgt gctttgccaa tgtttgtaat gagtccgaag
    24601 ttaggcaatg caatttttga cagctcctga atttcacctt gatgattggc tcccatttca
    24661 attactgcaa tttgatgttc actatttaat cgcaatagtg taagtggaac accaatatga
    24721 ttgttcagat tcccctcagt aaataaaacg ttatatgatg aaccaagtac acggcttatt
    24781 aattcttttg ttgttgtttt tccgttgctg cccgttaaac caataacagg tgttttcaat
    24841 gcttgcctgt gataggtagc taaatgctgc aaagtcgtca gtacgttctc taccaaataa
    24901 atattttcac cttttattga ggagtcatct actactgctg caagagctcc attttgcatt
    24961 gcttgtaaag cgaatttgtt accatcaaaa gaaggaccct ttaaagcaaa atagatattt
    25021 cctttgatta gtttccttgt atctgtacaa actcctgttg agctcagtaa taaactatgt
    25081 aaatgtgcta tcatttatat gcaataattt tcaaaaagaa agccccaaaa aaggggctta
    25141 tgtattttat ttaaacgttg aaggatatta tcctttctta gatgcttttt gattaaaacc
    25201 aacagggctg cctacacgta ccattgaaca acggaatcca atccaatcag ttgattgttg
    25261 ctgatctaaa aatcttcttg tgccaggtga catccaatat gctcggtctt tccaagagcc
    25321 acctttaatt acgtgtgcat tgtcatcaat caatgttgtg ccacccattt tatattcgat
    25381 attgtcaggt tcatctccat ctaaatcgtt aatgtaatct gctttagtat aatttctgcg
    25441 tgattcattt tctttttcat ctacttcgcg ccataccata cgaccaagac tatccttttc
    25501 tgcatattga ccttcttcat caagtaatgg tgttacaaac acgtttcctc tatacggatt
    25561 aaagtcatcc atgtcttcag gtgataatgg gcgataaaca tctttaaccc actcagaaac
    25621 gttacctgcc atgttgtata agccatagtc atttggcaaa taagaaaata caggagccgg
    25681 acggtctgca ttgtcattta atgcacctgc tgttcccatc atgtcaccac gacctctttt
    25741 gaagttggca agcatttcac ctttatattt ttcagaagag tttctgacta cgtgaccatt
    25801 ccaagggtat atttttctgt cggtaacacg ttcataaacg gtattgccaa tcaagcccaa
    25861 tgctgcatat tcccattcag cttctgttgg aagttcgtag cggggtaata agattccatc
    25921 ttccatacgc acattacgtt catcacgatt agggttcaaa tcctgtagtt tactttttac
    25981 taaaccttca tattgtccgg caagataagc atctgtatta aagttatctt cgtttacctg
    26041 agcaggatta acacgcaaaa ttccttcacg aactaaaatc tgttcattta cacggtcggt
    26101 acgccaactt gcaaaatcat tggcttgcaa ccaatttacg ccaacaacag gatagcgctg
    26161 ataggcaggg tgacgtaaat ataactcaac catcggttcg ttataaccta atttacttct
    26221 ccaaactaat gtgtcaggaa gagctttttt atacacttcg ggatagtcgg ttccataaac
    26281 acgatccaac caatagagat actctaaata atgtacattg gcgatttctg tttcgtccat
    26341 gtaaaaagat gaaacggtaa ctctgcgagg aatgttatcc cagtcataag gaatctcttg
    26401 ctctgtgcgg cccatggcaa atgttccgcc ttcaacaagg attaatcccg gacctgtttc
    26461 ctgctcggta tatggagtaa cttcaaatcc accattcttt tcgtcattgt acttccatcc
    26521 tgtggttgaa gatttctctt tttgcttgca agcatttgtt gccatcactg ctaatgcaac
    26581 gaaggcaagg gtttttgcat ttttacggag attcattttt acgtatttgg aaactttata
    26641 tggtttttgt cattaaacta aaatgtgggg caacttattg ccttgaaccg ttttttcttt
    26701 ggacggcatt caaactgcaa acctaaagaa atttcatgag atccggcagt aacattgtta
    26761 tttaatttag aaacggtaac atcataggaa taaccgaact taaacatgtc tttttgtaac
    26821 ccgatgaggg caatgaaact gtcgctcgac aggaattttt tacctgtata gcctctatac
    26881 cacaaaccac caactattgg cgacttgatg acataaagac caaagttaag ttggttaaat
    26941 ccttgttgtt gctgatacag aaagtttgga gagatgaaag taccttccaa tttgcctcta
    27001 taagttgaag cgccttttaa tggtaataac gcaccaacgt gtgctgtata ttttcttggt
    27061 agcggacttg aaccctcaac aataaatgcc tgatcgggct ctgtaatatg atgaacagca
    27121 aagccgccat aaactttaga gctgtagcct ataacaccgg cagaaaaatc agcaaaacct
    27181 cgtttttcgg caggacgtac ttcttgtgtt tcgaacacat aaccatagcg tggatcaatc
    27241 atatcgccaa aggtcagttt gtcccagtct aatttttcct gaacataggt agcttgaaga
    27301 cctgcacgaa tagagaaatt tcttgaaatg tttaagttat aggaatagat accggagaaa
    27361 ttggtagtat ttagtgttcc gttacctgct ttgtcattca taatgattaa acctaatcca
    27421 ccggagagtg cttcaacatg ctgatcgtat gaggcactta acgtgatgaa tgttttattt
    27481 aaccccggcc attggttacg atagttcaat gccagtcgtg gacaacgaac cgagccggca
    27541 aaagctggat tcagataaat cggattggca taaaactgcg aaaactgcgg gtcctggcca
    27601 aaacaaatag ctaactccag gctgataatc atggtaagta caatacgctt aatcatagtt
    27661 catttacttt tcaaacttaa ttttatacgt atagtactaa aatagttaca attctgcacg
    27721 aatatattgg tggttaaaaa tctaatcgga acaaagttag gcattaatta ttttaatatc
    27781 aaacaaaata aaatccaata ttgcatgttt catacatgtt gtattgttcg agaaacatat
    27841 ttttgaaccc aattaccaaa aaatgaaaag ttttttcaca ctattttttt taataggatt
    27901 gacaggtgtt gctgttgcgc aacaggatga atcaataaaa cgcacttatt ccatagattg
    27961 gcgcagtaat gttgtattga atggcgaaga caggctgaat tttaccaatt gtttttataa
    28021 acaggacagc aagatgccct atttctctac cggtctgcca ttagcggata ttactaaagt
    28081 cgcagaagta agtattgaaa accctgttta tgagccatta attagtaata atacgattga
    28141 tgcttcatcg gaaatacttc cggaaacaaa gctttcttac tttaaaggta aaccttcttt
    28201 gtatattgag tttgtacctg taagaaaaaa tgctgttaca gggcaaacag aaaaattagt
    28261 tgcatttaat cttaaggtga catcgggcca acagagtcaa aaaattggag ctgtctcaac
    28321 acaagtttat gcggcaaatt ctgtgctggc ttctggcgaa tggtataaaa tttcagttac
    28381 agaggatgga gtttataaaa tcacttacag ttttttaaaa gaccatttaa aaattgattt
    28441 gtctgcttcc tcacctcagt cattcagatt atttggcaat ggcggtggta ttttaccttt
    28501 attaaactct cagtttagat atgatgactt gcaggaaaat gctgtatttg taaatgatgg
    28561 aggtacagca ggaaaatggg atgatcagga ctatgtttta ttctatggac agtcaccggt
    28621 acagtggtac tataattcat cgcagaacag atattaccat caggcaaatt attattccga
    28681 ttccacattt tattttatta cgtttaatgg aggaaccgga aatcctaaaa gaattcagca
    28741 atcggcatca ttatcagtga cacctgactt tgttgtaaac agttttgatg attaccaata
    28801 tcatgaacta gataaaacaa attttgtcaa atcgggacgc gaattttatg gtgagtcatt
    28861 cgacattaac ctgaatcaga cattttcatt taactttcca aacattctgc aacaaacggt
    28921 gactataaaa acagtgtttg caggtcattc tcccggtgtt accagttcgg cttcagcacg
    28981 ctatcaaaat ataaccttgt tttcgggtac ttacacaacc ggttcacaat atacagatga
    29041 ttatgcaatg gagcgaacca caacttcaac attcttgcct tctgccggtg atgtcgtgaa
    29101 tattgatttt acatttactc cggcaaacac agagtgtatt ggctttttaa actatataga
    29161 attattggca aagcgtgcac ttacatttgc aggtaatggt actactgatc agatgttttt
    29221 ccgcagtatg gaatcttcag gtctcggaaa aactgcacag tttaatattg catcagcgcc
    29281 tgcatcaatg acggtatgga atatcacgga cagaataaat gtcagacaac aattgcacaa
    29341 taatggttcg tatattacac aggttgacag tacacagcaa tttatagctt ttggcggaaa
    29401 taatttctta acacccggag ccattggcgc agtggcgaat caaaatttac attcattgcc
    29461 tcaagccgat ttaattattg taacacatcc attatttgaa gatcaggcaa accggattgc
    29521 agatatgcac agaaccttgg acaatatgtc tgtgaatgtt gtgagaacag atttgatata
    29581 taatgaatat tcttcaggag cacaagatat tgcaggcatt cgcaattttg taaaaatgtt
    29641 atatgacaga ggaataaata atggaacgga gccaaaatac ctgcttttgt ttggtgatgg
    29701 ttcttatgac aacaaatacc gcagacctaa taacactaat tttattccaa cttttgaatc
    29761 tgaaaactct tattcgtttt taagttcatt tgttgctgac gatttttatg gtttaatgga
    29821 tgacaatgaa gggttatgtt caagtaacga aaaaattgat attggtgtag gtcggatcat
    29881 tgcacaaaac atcagcgaag ccaaaaccgt tacagataaa acaatccaat atgcatcatt
    29941 accacagaac agtggttgtc ccggttgtga tggcagtgga ggaactttag gcgactggcg
    30001 caatgtagta acatttatag cagatgatga agacgggcct atacatgtgc caccagccga
    30061 atcgtttgcc aattatttaa ataccaatca taaaacatat aacgttgata aaatttatct
    30121 ggatgcttac aaacaaatat caaccccggc aggtcagcgt tatcctgatg tgaatgatgc
    30181 tataaataag cgtgtcaata aaggttcgtt gattattaat tatgtaggtc atggaggcga
    30241 aaccggatgg gcagaagagc gcgtacttac taatgatgat attaactcat ggacaaatat
    30301 taataaacta ccggtattta ttacagccac ttgtgagttt agccgatggg acgatcctgc
    30361 aagaacatca tcaggcgaaa aaatattatt gagcgctacc ggtggtggtg tggctttatt
    30421 ttctaccaca cgcctggtat ttgccagcag caacgaagtc ctgaacagag ccttgataaa
    30481 gtttatgttt gatgatccaa cttatcgttt aggtgatata atggtagcca gcaaaaccga
    30541 tccgggaaat ctaaatctga atacacgtaa ttttagcttg cttggtgatc ctgcgttaag
    30601 attacattat cctaaattca acattgaggc tacacaaata aacacgcaac caattattgc
    30661 actaaacgac actttaagct cgttagaaaa agtgaccata gccggaagaa ttgttaaagg
    30721 tgggcaactt cagtctgatt ttaatgggtt tatgtatcca acagtttatg acaaagcttc
    30781 aagcattacc atgttgcgca atgatgcatc aagtcctaac ataacattta agcttcagaa
    30841 aaatattttg tataaaggga aagctacagt aaaaaatggt gaatggtcgt ttactttcat
    30901 tgtgcccaaa gatatttcct atcagtttgg tttaggcaag ctaagttttt atgctgaaaa
    30961 tggagtagaa gatggtgcag gatatttcga tagtatagtg gttggtggtt cgtctgcaaa
    31021 tattatttca gaaacagatg gacccggtat aaagctgtac cttaatgatg aacgctttgc
    31081 atttggtggt acaaccaatg aaaagccaac aattgtatgt ttgctctcag attcaacagg
    31141 tatcaatact gttggtaatg gtgttgggca tgacatcact gcaataattg atggtaaggt
    31201 tgaccccgtt tacgtactca atgattatta tgaagcagat ttagacagct attccagtgg
    31261 aagagtggcc tatccgctat caaaactttc tgaaggaaga cacacactta aagtaaaagc
    31321 atgggatatc ctcaacaatt caagcgaagc ttatacagaa tttgttgtgg cttcggatgc
    31381 atcattagca ctgaaacatg ttttaaatta ccccaatcct tttactacac atacacagtt
    31441 tacatttgaa tacaacaagc cttgtgaaga cttagaagtt caggttcaga tttttaccat
    31501 ttcgggtaaa ttggttaaaa caatttctaa aaagataatt actccgggta caagaattga
    31561 tgatttaact tgggatggtc gtgatgactt tggcgacaga attggccgtg gagtatatat
    31621 ttacagagta aaactcaata cagcagatga gtcggcttca gagacgcaaa aactggtgat
    31681 tcttaaatag aaaccttttt tattttttat cgtatatttg ccagccgctt cttaaaagta
    31741 acagcgtaaa ataaaaacca cttattgtcg ttattcaaac ttagctttta aaacttatta
    31801 ctgaattatt ttatacatga gatataaatt gttatacggt gctttatcat tagcagtact
    31861 tgcaatacaa aatcaggcat ctgcacagaa tgataaggcc aacctattag gtcagattaa
    31921 tactattaca acagcagttc catttctgat gatttctcct gatgcccgtg ccggtggtat
    31981 gggtgatgcc ggggttgctt cctcagcaga cgttaacagt attcattgga atcctgccaa
    32041 gctggtgttt gcagaaaaaa agatgggtgt cggaatttct tacacaccct ggttacgaaa
    32101 attagtaccg gatataaatc tggcttatgt aagttttttc accaagccta atgatgtttc
    32161 tgctattgga gcttcgttac gttatttttc tcttggaaat attgacttta cttctgaaac
    32221 tggcgatcca atgggatcat acaagcccaa tgaatttgct gttgatgtag cttactctcg
    32281 aaaattggga gatgaattta gtggaggtat agcacttcgt tatatccatt ccagccttac
    32341 aggaggtatc acagtaggcg gtaatgcaac aaaagcagga aactctgttg cagccgatgt
    32401 ttcattcttc tataaaacaa atctggaaat ttcaggaaaa gatgcagagt tcggtgcagg
    32461 tttagccatt acaaatattg gttcaaagat gtcttataca gataaaggaa ctaaagattt
    32521 tattcctatt aatctgcgtt tagggccttc tttgaagata aagctcaacg actataatga
    32581 gttggcattt ttggttgatt tcaataaact tttggtacca acgccaccgg tttattctga
    32641 ttccacaatt gccggtgaag agaacccgat tatttttgca ggaaaagatc ctaacgtagg
    32701 tgtggcacaa ggcatatttc aatcttttgg tgatgcgcca ggaggaggaa aagaagagtt
    32761 gcgagaaatt aatattgccg gagggttgga atattggtat gataaacaat ttgcatttcg
    32821 tgcaggttat ttctacgaga ataaaacaaa aggcaacaga aagtatttta ctttaggtat
    32881 aggtttacgc tacaatattt tcggacttga ttttgcttat cttattccaa cagagcagcg
    32941 caatccatta gaaaatactt tacgttttag cttgtcattc aactttgatt caatcaaagg
    33001 aagtgacagt aacgaataaa ttgtgaattt cagagttggt tttggttttg atgttcatca
    33061 gttagagccg ggcataccat tttttttagg tggtgtacgc atagaacatc atcagggggc
    33121 taaaggacat agcgatgctg atgtaattat tcatgctatt tgcgatgccc tacttggagc
    33181 agcagcactc ggtgatatag gcaaacattt tcctgatacc tcaaatgaat ttaaaggaat
    33241 agattcttca attttattgg caaaagttgt ttcaatatta aaagaaaagt caatagcaat
    33301 tggtaatgtt gatgtgagcg tagtattgga gaaacctaaa attgctcctt atattcaggc
    33361 aatgagagag aaattagctt cagtaatgca aattgaccta gattgtgttt caattaaggc
    33421 aactaccaat gagaaaatgg gttttgttgg tcgtgaagaa ggtgtagcag cctatgcagt
    33481 tgcgctgatt aaccggcagt aagactaaaa ttctgaggat cattaccgcg ttctgattct
    33541 attttgtaat aaaagacttc tgcaattttt cccgctcttg tttttgctgt atctgcattt
    33601 ctgccttcaa acaaactatc tactgtaact tcaaataatt ttaaccagtg tttgaaatgc
    33661 ttcatttcta taggtagctg tctgtgtgct tcaaatgcac taccattgta taacggttcg
    33721 ttgaaaagaa tactacgcca gaaaccatac atctttttca gatgttcggg ccagcggtct
    33781 tgtatgttgt tgttgaaaat cggacccaat aaatcatctt cacgaatagc actgtaaaat
    33841 gtatcaacca ttaatactac atcttcaggt gtaagtatat catgtttttt atcagtattc
    33901 atttttttga attttcttct gctgtaactt ctcagctttc gtctgaaaca atttttattc
    33961 ttctggctga tggttttttt aagaatgaat taaaaatacg atgtgattct aaatgattct
    34021 ttaaaaaagt tacgctaata atttgatttg tattgaatgt tgttgctttc ttcccaaaat
    34081 aatatcctat tgaaccattt tgtctgaata cgtaaaccca ttcatcgtta tcagccatcg
    34141 gataggcaag tgcagcatca tgcaagccat catcaacaac ctcaaagtag gcatcattaa
    34201 agcggttgtt atagttgtct ttttcatggc tatcaaggtc ggttccgttg cattgtttaa
    34261 tttgtttata aaagcagttt tcactgtagt tctcaagatt attgatgcaa cgacagaggt
    34321 tatattttgc agtgatgtgt gatagaaatt catcagcagc ttttcttgaa ccaaaatatc
    34381 ttaacggttt taaacttgcc tttgcaatgt cattaattgt aaatcctaac aggccattta
    34441 aattagcatt tatataaacc ccgaaatttt tttctgattt gccggcacgg ttaaagaaag
    34501 gttgatattc tttaatgtat tgaatttcga gcagacatgc cagcaattca catcctgtat
    34561 tcatgtagca tatatcatgc aagagtgctt ttattttttt tgatttggaa ttgttgttgc
    34621 gaaaatgtgt ggtaactctt tttttaatat tttttgcttt gccgatatac agaatttcat
    34681 gattgctgtc tgtaaagaaa tatataccgg gtacttcagg caacttgtta atgagttcgt
    34741 atggaatttc agtattttta ataggcacac tgcgatgaag caacgtatgt tcaagacctc
    34801 tttccgaaac caatttctct aatatttcac ttgctgcaat agcatcgttc atggcacgat
    34861 gatgaccttc taaaactatt ccaagatttt ttgtgagatt gcctaatgag taggagccaa
    34921 aaccggggaa gaattttcta ctcaaagaac aagtgcagag tacttctgca ttaaaatcta
    34981 ttccacatcg tttaaattca gctttgatga aactaaaatc aaaagaagca ttgtgtgcca
    35041 caaaaatttt tccatcaagc aattgaagca gctgaccggc aatttcctta aagcatggtg
    35101 cattttctac caattcattg gttatgcctg ttagcccaat gataaaagac ggaatgtctg
    35161 tttcaggatt aatcaacgta gaaaaacggt gttcaatagt ggtgccatta tgaattacaa
    35221 ctgcaacttc agtaatccgg ctgccttgtt ttaaacttcc ccctgtagtt tctatgtcaa
    35281 caactgcgaa catgccgtta ccgttagtta tttttaaatt tcaattttat atgcgcagtc
    35341 aaaatgacct aaagggcaag acttgtaacc atgcaatccg caaggccggc aactcatttc
    35401 accaacttgc ttgatgacac ttttgtcaga caacggccca aagccaaact caggtactgt
    35461 agagcagaaa aatgcagtca cgggagcatt aacagctgat gcaagatgga gtggggcaga
    35521 gtcgtttaca tagttcattt cagcatgttg cattaaggct gccgactcgg tcagtgttaa
    35581 tttaccacat aatatttcaa ttttgggatg tgatgaaagc aaccttattt cattacaaag
    35641 tgcagcatca acaggtgcac caattaaata tatttttata ttgtcagaaa tagtcagaat
    35701 taattctgcc cacttggcta tgggtaattg tttggtaaac cagacagagc ccggtgccat
    35761 acagacaaat ggttgtgatt ttaatggttg tactttttga atttcttccg ctgtaaaata
    35821 caaatgtggc ttttgatagt gaatattttt caggtcatca atcaatgaaa agtttcgata
    35881 tgtttcatgg tttttgccaa tgaaatgttc tgtttttttg tgataacaaa atgataatgg
    35941 atttttatca tagcctcttt tattttttga ttttgcacat agtgctataa gacctgtgct
    36001 gaaaaaccgt tgcaggttga ccaccaaatc gaatctttct tttctgattt gaccaataag
    36061 ttttatgaga ttagagaact tatttttctt tttatcccag atatataatt tattgacaaa
    36121 ggggtggttt ttcaataaag attcattgcc ttttcttagc acaaaatcaa tctgagcttc
    36181 cggaaatgcc tgatgaatgg attcaatcaa gccggtagcc aatattacat ctccaatata
    36241 tgctgtctgt acaattaata ttttatgcat acttatatgt ctcagactgc aacatcatat
    36301 tcacgcaatg catcgtttag tgaagtttta ttgtcggtag attgctttct tttgccaata
    36361 atgagtgcac atggcacctg aaaggttcct gccggaaaag ttttggaaat tgaaccgggt
    36421 atgaccactg aacgtgcagg tacaactcct cgatattcaa ccggtgcacc tcctgataca
    36481 tcaattattt ttgttgacaa agtcagtaca acattagccc caagcactgc ctctttttca
    36541 acacggacgc cttcaacaac aatgcatcgt gagccgataa aacaattgtc ttcaataatt
    36601 accggtgctg cctgaacagg ctctaaaaca cctccaatac caaccccccc gctaaggtgt
    36661 acgttttttc ctatctgtgc acaactgcca acagttgccc aagtgtcaac cattgttccg
    36721 ctgtcaacat aggcaccaat atttacaaat gatggcatca tgatgacacc ttttgcaaca
    36781 tagcttccat agcgtgctat ggcatgaggc actacacgaa cacctaaatc ttcatatcct
    36841 gttttaagat ctattttatc ataaaactcc attggtcctg catgatgtac agccattttt
    36901 tgaatcggga aatataaaat gacagccttc tttatccatt catttacatt ccaactatta
    36961 ttcaatggct ctgctacccg aattttacct ttatcaaggt cttcaataac tgatcgtatt
    37021 acatttttgt tggattcatc gttaagtaac tctctgtttt cccaaatatc attgacaatt
    37081 ttttctctga ccggcataaa tacttttttt cagctacaaa tataactacc ataatgcatt
    37141 cattgcccgt tgttttttat ttttgcatct ttcaatacag acatgccgcg catacttgca
    37201 gttgattatg gaaataaaag agtaggtctt gcagttacag acccaatgca gattattgct
    37261 acggccttaa ctacagttca ttcctccaaa gcggttgaat ttatcagtga ttattgcaat
    37321 gctgaagtag tagaagaagt tgttgttggc aaacctgtac aaatggataa tacgccctct
    37381 gaaattgaga aaaatatttt gctgttcatt aagaaactta gtgaaagatg tccaaatgca
    37441 aaggtcgtta gatatgatga aaggtttacc tcaaaaatag cacaacgaac attgttggaa
    37501 gccggactta aaaaacaaca gagacaaaat aaagcattga ttgatcagac cagcgctgtt
    37561 atactgttac aatcttactt agaacataaa agaaactttt tgaaaatatg attttaccaa
    37621 ttattgccta tggcgacccg gttttgaaaa aagtgggatt accaattgac aaagattata
    37681 aaggtcttgg acaacttata gaaaatatgt ttgagacgat gtatgctgca tccggtgttg
    37741 gattggctgc accacaggtt ggcatttcca tcagactatt tgttgttgac accaaacctt
    37801 ttgcagaaga tgaaccggag ttagaaaatt ttaagaaagt attcattaat ccacaaatta
    37861 ttgaagaggt gggtgatgag tggaagttta acgaaggttg tttaagtatt cccggaatca
    37921 gagaagatgt gatgcggaaa aaaattatcc atctgcgtta tcaggacgaa gaatttaaaa
    37981 cacatgaaac agtttttgaa ggattggcag caagggtaat tcagcatgaa tacgatcata
    38041 ttgatggtaa attatttgtt gagaaattgt cgccttttaa aaagcaactg ataaaaggaa
    38101 aactgcgtga cattactatt ggcaaggttg atgttgatta caaaatgcgt ttcccaataa
    38161 agaaatagta acaatgttaa tatttctgat tggttttatg ggagccggaa aatcttctca
    38221 ggcaaaaaag ctagctgcaa gattaagttt tgaagctgtt gatctggatg aattaattga
    38281 aaaagcaaac aaacaatctg tttcagaaat ttttaattta aagggagaac aatttttccg
    38341 taaagttgag tgtgaaactt taagaacaat atctgaaaaa caaaatgcag ttgttgctac
    38401 aggtggtggc acaccttgct tttttgataa tatgcaatgg atgaatgaaa aaggcattac
    38461 tgtttatctg aagatacatc cggggacagt atatcaccgc attgcaccat caaaagctaa
    38521 acgaccattg attagccaca ttaatgatgc agatttaatg gagtacatta tggaaacaat
    38581 gactgaaagg gcgcctttct acaatcaggc aaagattcag atttcaggac tgaatcttaa
    38641 aacagaagac cttgtgaatg cgcttcagcc atttttagag cagtaatgca atagtattgc
    38701 aggttaacag tttttattta tgtttacagt tcaaaacaaa agattatgaa aatattatta
    38761 ttctttttac tactgccaac catgttagtt gcgcaaacat ttcaaaaaaa tcaaggtgag
    38821 agcaatgaag attttgttaa gcgaattgca ccgcaatcgg ctaatgaagt taaatatatg
    38881 ctcagtgata cttttaatac atctacacaa aaagttgttt atgcttatag catttttgaa
    38941 cacggcaatc tgataaccaa tagcgacagt actttgactt attttttatg tattcttgtt
    39001 cctacctcag taggtgcaat ggattacaat atgcaacagt ttcgtatggt agcaagttat
    39061 aaaaagaaag ccaggattga gagtgcagag attataaagt cgggaaggga aaagaaactt
    39121 caggtatata ttgcagaatt ggtacggggt cccggaggtg tgcccagaga tgtgaaacgt
    39181 acttttgttt ataagcagga aaaagaaagc agaatgttta ctgatacttt cagagaggta
    39241 ttgggcgatt attaaaaaac actttatgaa taaggcacac gaacttgatt ttaaaatttt
    39301 tggtgatgac attcaggctt tggaagtgga actcgatcca tcagaaggag taagggctga
    39361 agtaggtatg atgatgtata tggaagatga tatagaaatg cagaccaata ccggaggtgg
    39421 aatttttaag ggattgaaga gaatgataac cggtgaaagt tttttcatca ccaactactt
    39481 aaatcatgga agacaaaagc gtaaaataac ttttgctgca ccttatcccg gaaaaataat
    39541 tcctgttaat cctgctgaat atggaggacg ttttttatgc cagaaagacg gatttttatg
    39601 tgcagcaatg ggaatcgaga ttaatgttgc ttttacaaag aaactgggtg caggactttt
    39661 tggtggcgaa gggtttattt tacaggagtt agcgggcgat ggctatgcct ttattcattc
    39721 tggaggcaca attattaaga gagagttggc tgttggtgaa agacttcgtg ttgacaccgg
    39781 atgcttggtc gcctttgcac caaccataga atatgacatt aagtttatag gaggttttaa
    39841 aaatgttctt ttcggaggag agggattatt ttatgcacat ctaactggtc caggtacagt
    39901 ttatttacaa agtttaccct tttcaagaat ggctgataga attattgccg catcaaaatg
    39961 gggtggtgca agggaagaaa acaggggaat aggagggctt ttgggtggat ttatcagtgg
    40021 taatgaataa gctgtttttc tttgttttat aaaccgccca ctcaccacaa ttttaaaata
    40081 cttggtgtaa aatattcaat agcgaaacca atttaggtta ttttcgtata gtttttatta
    40141 aaccaccaat gacaaaggaa gatggcatcg caatttatcc cggttgattt taaccctttt
    40201 gaagaagtta ttccggacaa tatttttacc acatcagaag agcaacgtga aatctggaca
    40261 tccgtacaaa taggaggtga ttcagcatca ttagcctata atgaaagtgt tacactttat
    40321 ttgaaaggct tgtgtaatca tcagcaactt tcagaagcct gtaaaagtgt tgttgaaaga
    40381 catcaggcac tgcgttccac tttcagctca tcaggcgaaa catttcaggt gcatgaacat
    40441 gtaaatattg aaattccatt tattgattta actgacctga gtccgacaga taaagatgca
    40501 cgcatacttc atttttcgca gcgtgaagta tccattccat tcgacctgat gaatggtcct
    40561 gtcatccggt ttgcgatttt taaaatctca aacgctgata cagcattagt catcacagca
    40621 catcacattg tttgtgatgg atggagcatt ggtatcatca tgcaggacat cagtaaattt
    40681 tattctgcct ttgtaaataa tacacaacct gaccttgagc ctgttgtgcc gttcagtaat
    40741 tatgttaagc gacaggaaga gagaaaactg aacggagagt ataaacgtac agaagattat
    40801 tggctcaatt tatattcaaa agatgtacct acagtcgatc ttccggttaa caaagcccgc
    40861 ccggctgcac gtaccttcaa tgcaaagcga tttgatataa ccattgatgc cggcttagta
    40921 agtgatttaa gaaaacttgg tgcaaaaaat ggaagcagtt atgtcaatac cttgtttgct
    40981 gcatttgaag tttttctttt tcgtttaact tccaatcctt cattgatatt gggactacct
    41041 gctgccggac aatcggcttc gggaatgtat gccatggttg gtcattgtgt taatctgtta
    41101 ccaataaaaa gttttgttga tgagaatatc aacttcggtg aatatctgaa aaccagaaaa
    41161 cctttaatgt ttgatgcatt cgacaatcag gaatttacga tgggaagctt gctcggaaaa
    41221 ctgccactgc agcgcgaccc ttcaagaatt ccattagtac ctgctgtgtt taatgttgat
    41281 cttggcatga cgcaaggtgt ttcgtttcat ggtttgaact atgaatatac ctctaatgcc
    41341 agaaagtttg agaacttcga attgtttgtg aatgcaaccg gtatgggcga taagctcatt
    41401 attgaatgta cttacaatac cgaccttttt gaagatgaga tgatgcaatt gagaatgcag
    41461 gagtttgttg tgcttcttaa tggattggtt gaatatgcag atagtccgat aaaaaatttg
    41521 ccattactga cagacgatga gaaaagaaaa ttgtttatca aatggcctgt tgtaaaagag
    41581 aataatctta ggagtgaatg tattcatact ttgattgaaa agcaagcatc tgcacatcct
    41641 gatgcaaatg caatttggtt taatgaacaa acagtaactt acgctcaact taaccaacga
    41701 gccaatcagt tagcacatta tctaataaag gcaggtgtta aacaggaaac attagttgga
    41761 atttgtcacg acagaacaga cgatttaatt gtcgctatgc ttgcagtatt aaaagcaggt
    41821 ggtgcctacg tgcctttaga tccaaattat ccttccgata gaattgctta tatcatgagc
    41881 gatgcaaaag cgccactctt gattacctct tcaaaatacg caaaacagtt aggcgaaaca
    41941 ggaacaagat tgatttgctt agataaagaa gtaactgaaa tttctattga gaaaacggca
    42001 aagcctgatg ttaaaatttc gtgtgatcag ttagcatata ttatttacac atctggttca
    42061 acaggaaaac ctaaaggagt agctatcgaa caccgaaatg ctgttgcctt aattgattgg
    42121 gcacagacag tttattcttc agaggaactt gcaggtgttt tagcttctac atcaatctgt
    42181 tttgacttat caattttcga aatatttttt acattaagca gcggtggacg cattgtttta
    42241 gtaaaggacg ccatggcttt aaatgcctta tctgaaaagg caggtgtaac attggttaat
    42301 acagttcctt ctgcaattgc agaacttctt aaacttaaag gcggagttcc atcaagcgta
    42361 aaaacaataa atcttgcagg cgaacctttg tcggttgaac ttgttcagaa aatttactcg
    42421 caaacaacag tgcataaagt gtatgatttg tacggaccat cagaagatac tacctacagt
    42481 acatttacac tcagaaaatc tgatggccca tatacaattg gaaaagccat caccaattcg
    42541 caggtttata ttttagataa aaacagacga cctgttccta ttggtattgc cggtgaactt
    42601 tatatggcag gcgatggtct tgcacgtggt tatctctata aacctgaaat gactgaagag
    42661 cgttttgtgg ataatccttt tgttcccgga caaagaatgt acagaaccgg tgaccttgga
    42721 aagtttatgt ctgatggaaa tattgaattt ttaggacgca tagataatca ggtaaagatt
    42781 cgcggattca gaattgagct tggtgaaata gagtcaatgt tgcgaatacg aaaggagata
    42841 aaagaacttg ttgtaatggc acgtgaagat caacccggaa ataaaatttt ggttgcatac
    42901 ctttctgcta atgctgaaaa agagattgat gtcaatgaac tacgacagca tctgcgaaca
    42961 gagcttccgg aatacatgat accctcagta tttatgattc ttgaaaatat tccgttgaca
    43021 cctaacggaa aaattgacag aaaagcattg cctgttcccg aagtggaagt taaagttgaa
    43081 agtgcaaatt ttgaagagcc tcacaaccct ttagaaagtt tgcttgcagg tatttggagc
    43141 gaagtgctgg gaattgataa aattggtatt catgataact ttttcgaatt gggaggacat
    43201 tcgttgcttg gaattagaat gatgggagcc atagagcaga agacaggagt taaattagat
    43261 tatccaacat tattccgtgc ttcaaccatt tcgcaattag ccaaggtgat gaatgcagag
    43321 gagttgcata ttgactggcc tatagtggtt cccttacagc ccaatggaaa agaaagacca
    43381 ttgttctttg ttcacatgca caacggaaac attcagcgat ggagagtact tttaaagcat
    43441 ataaataaag accaacctgt ttatgctatt caaccacgcg gacttgatga aaaatacgac
    43501 tatcattatt ctgttgaaac gatggcatca ttctatattg atgagatcaa aaaaattcaa
    43561 cctgtaggac cttatcgtct tgccggatta tgttttggtg gaactactgc ttttgaaatg
    43621 gctcgtcagc taaaagcaaa aggcgaaaca gctgaacttg tttttatgat taataactat
    43681 gcaccaccat ccaatccaat gcaatataaa atccgtgaaa catgggatga gtttaaaggt
    43741 atgactttgg aagataaact tgaatttgca cttgaaaaaa ctaaaaatgc cggaaagaaa
    43801 gtattaagca agctgacaaa ggtttttgat tccggcaata ctgcaacctc tgaagttaaa
    43861 gtaaatacta agcctgatat tcgttgggta aattcagtag ccttactcaa ttatcagcct
    43921 gaaagcgaat attctgaaaa agtagttttg attaaaaccg gagaagaaat tgccaggcat
    43981 tatgatgaga cattaggttg gaaacgactg ataaatggtg agattgaaat gcatcagatt
    44041 cccggaagcg ataacgatac catcattacc cataaggaat attacactca gttagcacag
    44101 tatttaaatc aggctttaaa ccaaattaaa tgacaactga aatggattta cagataactc
    44161 cggaagtagt tttttgtaat gacttattaa ttcaggataa tgaatgtttg gtttttatat
    44221 tctcttctga ctcgtttgca gcagtcgagg atgatttgaa aaaattatta agcaaagaag
    44281 aaacagagcg atttcaaaaa tttaattttg aaaaggacag acaaacctat tgcattgcac
    44341 atggattgaa gagagtacta cttgctacat tactcaatac aaagacgcac cttgagattg
    44401 ctgtacacct tagtgggaag ccatttgtta aggagcaaaa aagcagtatg gtgttacatt
    44461 ttagtttgag tcattcagaa aaaatgatag ctctggcatt tcagaaaaat aacccattgg
    44521 gtgtagatat agaaaaaatt caatcggcag ataagtacga aaatgtgatt cagtattatt
    44581 ttacttctga tgaacaaaag caaattgccc ggtcagacag cgcaacattt tttaaaatct
    44641 ggacacagaa ggaagctata gggaaagcca caggtcaggg gattgctaat ttgcctgaaa
    44701 ttaccgctcc gggtgaatat gatttaagta cggtgtcttt agacggatat gttttaagta
    44761 catgtttttt cagaggtaat gagattagtt ttaagacaat tcatgcgcaa gatgttttaa
    44821 atcagattaa aatactatct ttgcgcccgt aattaacatt ataatcaacc ggataatttt
    44881 ttgaaaaaca ctgataattc cggttcttcc atcttcaaat gaacaagact tctttccatg
    44941 agctttcgtt aagtcatgaa atgctccagg ctattgacga aatgggcttc accgaggctt
    45001 cgcctataca agcacaagct atacctgtat taatgcaggg tcaggatgta attgggcagg
    45061 cacaaacagg taccggaaaa actgcagctt ttggtattcc tttgctcgaa atgctcgacc
    45121 ccaaagtaaa agctacttct gcattggtaa tgtgtccaac ccgtgaattg gccgttcagg
    45181 ttgcacaaga aattaaaaaa ttagccaagt ataaaaaagg cgttgttgtg cttgccgttt
    45241 atggtggtga gcaaattcaa aagcagatta atgcattaaa acgtccggta catgtcattg
    45301 ttggcacacc gggaagaatt atggatcatt tggacagaag aaccataaac ctgtctaaaa
    45361 ttcaaatggt agtgcttgat gaggccgatg agatgctgaa tatgggtttc agagaagata
    45421 tggaagcaat tctgcaacat gcacctgacg aaagacagac ggtgttgttt tctgctacca
    45481 tgcctaagcc tattctggaa atcacaaaac gttttcaaaa caaccctaaa ttagttaagg
    45541 tcactaaaca ggaattgact gtatcatcta ttgaacagat ttattacgat attccatcgt
    45601 cacaaaaagc tgatgtaata acacagttag ttgagttaca caatttgaaa ctgatgcttg
    45661 ttttctgtaa taccaagcgt aaaacggatg aggtaaccga gcagtttcag caattgggtt
    45721 acaaggcaga agccattcat ggcgacttgc gacaaaatca aaggaacaat gtgttggcac
    45781 gattcaaaaa aggtgatgtg aatatgctcg ttgcaacaga tgtagctgca cgaggaatag
    45841 atgttgagaa tgttgatgca gtattcaatt tcgatcttcc acttgatgaa gaaaattatg
    45901 tgcatcgtat cggaagaaca ggccgtgcgg ggcgttcggg caaagctttc agttttgttt
    45961 ccggaagaaa tgatatggct aaaatccgtg agatagaaga ttattcgaaa gtgagaattg
    46021 aaagacatca actgccatca ggaaaagagc gcattgagtt ggcaaaaatt aaattggcgg
    46081 caagactgaa agtgctcatt gaggaaggcg atatggagca gtacgacaat atgtttgcaa
    46141 ctttcaaaca ggaaggttta acattacatc aacttgcagc agcattactt aaaatgcatt
    46201 tcgcaccaat gttgcaacag agtcaaagta agtcaagcag taaatcttcg ggtagagaag
    46261 aacgatttgg cagagaggaa cgctcaggca gaggtaaacg cgatgatgga ggcggaagag
    46321 gtaaacgggg taagcacagt ggcgaagccg atatgatccg tttgtttttg agtgtaggta
    46381 aaaaagataa agtcagtaaa ggtgatatag taggtgctgt atcaggtttg agtaatatga
    46441 gcagcagcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtaaaggtga
    46561 tatagtaggt gctgtatcag gtttgagtaa tatgagcagc agcaacatcg gtgttattga
    46621 tatttacgat aagttttctt ttgttgaagt taatggtgag tttttaaatg atgtactttc
    46681 caacgttaac ggcaataaaa ttaaaggccg taaagtaaat gtcgaaatag ctaaagacta
    46741 agctactgtg taagccagca aaaagtcttt ttctcttcta tatttaatgc taattgtatc
    46801 tttttgactg tgttgtacta ttgcatgcac caaaccttca tttgaacatt caaatggttg
    46861 aatctgtatg cagtttttaa agtctattcc tgatatatta aagtgtttta taacagattc
    46921 tttaatgcac caacacatta gtgcatgcag attttttttc ttttgagaaa gaaatgcaat
    46981 ttccgtagca tttagaaaat agtcaattcc agcttcaata tttctatcgg tcatttcaat
    47041 atcaataccg gcactttttg attccgtaat tattaatgca gtataatttc cggagtgtga
    47101 aaaagaaatt gcatggttgc catcaatgaa tggtttacct gtttcttgat agtgtaattg
    47161 aagcgtactg ttttttgtta cttgtctaag caactttata ttaatttctt tttgaaacag
    47221 tttccgactt tctttatctg acggataatt ggcatgcacc acatacaatt gattattgat
    47281 gttgatttca ttcagaataa aagaaaactc catttgcatg gtgctaaaat agcagttgtt
    47341 tgttgatgat gtttaatttt gctgcatgac aactcctgac gtttttcaat ttggtattca
    47401 ggttacatca gaagtagcaa cggttcagat aggagaagca tcaatgacat actttaaagt
    47461 aactaatatt gatgccttgt atgcagcatt aagccagctt gatagtaatg atgataatgt
    47521 gatggatgag cgcatacctt attgggcaga gctttggccc tcggcaattg ctttggcaga
    47581 atatgtaagt gaacttgaga attctgaagt ttcgtttatt cacgaaatag gttgtggtat
    47641 ggcattgcct tcggtgctgt gtggcaaact gggtcataaa gttatgatga gcgattatgt
    47701 agaggcgcca ctacaatttg ctgaggcaaa ctggcatctg aataacacct ctcaaccgct
    47761 gaccatgatg cttgactgga gaaatatacc agacaatatt gaaaaatgtg atttactcct
    47821 ggctgccgat gtagcctatg aaggcaggat gtttcctgcc gtgataaatg ctttccaacg
    47881 cctgactaaa aatggaggaa aaattatttt caccgaaccc aacaggaagt tcacatcaga
    47941 attttataag ttattactga ataattttac taaaattaat tccttcgaca gattggtttt
    48001 cctcaacaac catcagcata aaatcaaact ttctgtcata aatgtttgat atttggatct
    48061 gagattttta aatggttttt aaaaaaccgg ccaatttttt gccgataaga atattgtcat
    48121 attgtgttct ggcaagtatt tgtgcttccc ttgcaatgtt gtttaacagg ttaggattgt
    48181 ttaataatga aagaatggtt tttgtaaaat cctccggggt atcggcaata agaatatttt
    48241 ttttatcagt ataattaatc ccttctgcac cggttgatgt tgatacgata acttttcctg
    48301 cggcaagacc ttcgagaatt tttattctaa taccaccgcc actcagtaat ggtacaacca
    48361 taatctgttt gttttccata aacttcaacg catcttttat ttcacctgca acaatcaggt
    48421 tttcagactt aatattgaaa tacttcggat tcatgttttt accggccaga aacaaatgca
    48481 ccttatttga tttttgttga atcagtggcc agatgtcatt caatagccag tcaacggctt
    48541 caatatttgg gagccagtcc attgatccaa gatgaaataa tgaaagactg tcagcatcca
    48601 tctgttcgat gttcttgtat ttggcagtat tcattcctgt tggtgccact tcaataggaa
    48661 tattacagcc atctttcaga aaaagatttt tatctagctc cgatatagca acgattgcat
    48721 caaagttgtt tatcaccttg ttctcatagc gtttaagtct gtcggataaa aaataaagat
    48781 aagttttttt taatggattt tttgttgctt tagcaagtct tttccagata agccattcca
    48841 cattatgcgc ccgcaatatg cacttggctt tgctgaatgt acgcactgtt tgtaggtaag
    48901 gactaagaaa taatccttca aacaatatga aatcaaatgt ttccgattgc agcagttctt
    48961 tcagcttttg gtgaaacgtt tccgtgtcaa aacggacaat gttatatgac tcgtttggtt
    49021 tgaacagatt ggtaaaagca tcccatgcgt ttacggaggc atcaagaaaa actgtttcta
    49081 atttgtattt ttcaagcaat gct
//
LOCUS       DAOQNP010000014        44292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_253_length_44292_cov_8.797658, whole genome shotgun sequence.
ACCESSION   DAOQNP010000014 DAOQNP010000000
VERSION     DAOQNP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 44292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44292
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_253_length_44292_cov_8.797658"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1580
                     /locus_tag="PKY09_03440"
     CDS             18..1580
                     /locus_tag="PKY09_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNB11939.1"
                     /translation="MINLQNTFPLFSKKLMNAMFIMPLVFLMLFVSNVWAGGNDTLAA
                     NVDENVHVAEKPKDFEIALQPALLSDNSTSQIRAGLIYDCTTNTIVWEKDLKYAYPIA
                     SLTKMMVALIAIEEIKCGTVDWQDQVSVTKTYRKSKRSKRTYTVNETYSLESLLKMAM
                     IPSNNEACGLIGKHISGSVETFVTRMNQKAAALGMTQTFYSNPSGLPAGYGSLDNSSS
                     PYDLLKLSTELIKFPELLSITNIAFAEVENGHGSNVYRNHNHLVMDYPGEVDGLKTGY
                     TKNAKFCLVATAKKNDHRLIAIALGASNPWLRNQIVGEMLNNYYTQLGFGPMGNQKVD
                     PLFAKADRKTVNDIASSNVPVMNQEIVYKTVTSIQKRAHIVKSGQTLSDIADKYNCSV
                     NELKKWNGLKSSRILKNQRLYVRVHVKKQIPVSTKVIDNYDECEDNAEDCAPQNVADK
                     QETPKKEQLPTSQSKPALKVIYHEVQPGDTLWKIAQQYPGTSVDQLKKINKISNSKDL
                     KAGSKIKVSVKG"
     gene            1603..2151
                     /locus_tag="PKY09_03445"
     CDS             1603..2151
                     /locus_tag="PKY09_03445"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HNB11940.1"
                     /translation="MKIKLLVTSLIVVFSVGRINSQTVSYPVDNDSKLICYTKVKELA
                     GVSKNDIFDRALGWINSYYKNPADVLRENDKANGKLLCKARFKLMGEPDKSGFQKDQG
                     NVQYSLIIEAKEGKYRYKLTEINWKQPSYFPVERWMDTKNQYYTKDWDWNLKYTDEQS
                     HKIIAELEKYMSNPPKVKKDDW"
     gene            complement(2153..3358)
                     /locus_tag="PKY09_03450"
     CDS             complement(2153..3358)
                     /locus_tag="PKY09_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB11941.1"
                     /translation="MKFYSADFVFPVCSTPLKDGIVVMDNSGVVIDVFENIASLNNAL
                     QIQIERSAIQKFHGMLTPGFINAHCHLELSYLKGEINKHNKLTGFIKDLVSVRMKAEH
                     DKIINTIKSAEESMLEVGIVAVGDISNTSITFQLKNKSDLQYHTFIEAYDLFELNTDK
                     VWNMAKELKETLQLNNRNSKCSISPHAPYSVTPHLFQKINANKPSIITIHNQETASEN
                     TLFETGTGDLKNFFELSGNKLPHIKPNGKSSIQNYVPWLNLCDEIIFVHNTFSSDDDI
                     RYAIANTKKCWWCLCPNANLYIENTLPEIERITKATENILIGTDSLASNNQLSILSEL
                     KIITKKFPQISLHQLLTWSTINGAKALKMDSELGTLEKGKKPGLVLIENVDLQNQKIT
                     ESSQSKRVA"
     gene            3416..4456
                     /gene="rlmN"
                     /locus_tag="PKY09_03455"
     CDS             3416..4456
                     /gene="rlmN"
                     /locus_tag="PKY09_03455"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNB11942.1"
                     /translation="MKSTPNIRELTFDQLSEAIAEFGEKSFRAKQIYDWLWNKSARSF
                     DQMNNVPLSLRNKLAEKFAFTPICVAHEQRSNDRTIKYGFKLHDGHLIEGVLIPTASR
                     MTACVSSQVGCSLSCKFCATGKLDRVRNLNADEIYDQVAIIREQSMAVYNIPLSNIVY
                     MGMGEPLLNYANVLSSIERITAANGLGMSPQRITVSTAGIAKMITKLGDDEVKFNLAL
                     SLHAANDSKRDKIMPINEHNNLEVLSDALKYFYDKTGTRVTYEFIVFNNFNDSLKDAQ
                     ELAEFAKHVPCKINLIEYNAIGDGVFEKARLDRLEVFKNYLESKNLVVNIRRSRGKDI
                     DAACGQLANKNQ"
     gene            4507..5484
                     /locus_tag="PKY09_03460"
     CDS             4507..5484
                     /locus_tag="PKY09_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB11943.1"
                     /translation="MTLSLDQIKKPISLEMDSFENKFRDAMRSKVSLVDKIMHYIVKR
                     KGKQMRPMFVFLSAGVCGKINESTYTAASLIELLHTATLVHDDVVDDALQRRGFFSIN
                     AVWKNKIAVLVGDYLLSRGLLIAVQHKEFKLLEIVSNAVRDMSEGELLQMEKARRLDI
                     DEDVYYKIIRQKTASLIASCCSCGAASVQADDDRIELMRELGETIGLAFQIKDDLFDF
                     EKSISTGKPSGIDIRERKLTLPVIYALSQASSVEKRTIINLIKNDHENESKVAEVLAF
                     VNKQKGIDYAREKMFELRNHAFEIINSFPDSDYRKSLHDLITFTTDRNY"
     gene            5488..6480
                     /locus_tag="PKY09_03465"
     CDS             5488..6480
                     /locus_tag="PKY09_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB11944.1"
                     /translation="MLKKGIIASLICIGSLLLLLYFTSLFSISELIYPNRVTVKSLTS
                     EFHNLKKNELVIDKDITVSPSQLNLTYRNFDVLIHDQFTLHGWYITGEQPAKTTVLLL
                     HDWNESKLSMIKAAEALYQLGFNICIIDLPAHGESDGEKFIIDQSMNHCLEVVADSLF
                     CLYETDNLAVVANGLSGIVAASWTKVDGRNQCLILVDPVPGMTAKINELVKEKWNGLS
                     MLISQFAQIMYHQKTGIDADSLNLQEYLGQLRTPLLVAISDSATDIEIIESVRIFESA
                     KSPNKKIWTNQTRSFIRIDTDAEKNMFRAMAAFINSNISSDKPKIKVRKRIAAL"
     gene            6477..8423
                     /gene="dnaG"
                     /locus_tag="PKY09_03470"
     CDS             6477..8423
                     /gene="dnaG"
                     /locus_tag="PKY09_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNB11945.1"
                     /translation="MIPKETVELIIETSRIEEVVGDFVSLKKRGANLLGLCPFHNEKT
                     PSFTVSPAKGIYKCFGCGKAGDSVRFMMEHEAFTYPEALRYLAEKYKITIEETIPTVE
                     EIQKQDERESLFVLYSFAQKFFSEKLFNTEEGRAIGLSYFNERGFNEAVIEKFQLGYA
                     PDQWDALSQAVTENKFNRQLYEKSGLAVVKEQEQDSQKVYDRFRGRVIFPIHNLSGRV
                     IAFGARQLANDPKSPKYINSPETDIYHKSKSLYGISFARKSIIQHDECFLVEGYTDVV
                     SLFQAGVENVVASSGTSLTPEQIRLIARFTGNITILYDGDPAGIKASLRGIDLILEEG
                     LNVKVVLFPDGDDPDSFARKNSTIQLIDYISKNAVDFIGFKTSLLLEEVANDPVRKAA
                     LIRDIVESISRIPDGIKRSLYTSQCATQMDVPEQVLITELNKIRRNLYKKDNEHQQAE
                     LVEKLIEMEYLPQQTILTETNAWYQEQEIIRLLLNYGDKNFNYQGGEQQKTVREFILE
                     QILPDGFGFDDEIFAGVFSYLMSMSTADFLSMLKTENSSDLEKLRSKTHELLMNKYEL
                     SDWESKGIYVKTEENNFNEAIMNPINHIKLKRVQILKRKNSLLLKEESNISDERMDEI
                     LSKQKVYETYINALSKLLGIVTLE"
     gene            8433..8957
                     /locus_tag="PKY09_03475"
     CDS             8433..8957
                     /locus_tag="PKY09_03475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNB11946.1"
                     /translation="MITLIAGTNNKNSHTEKLARIYYQALLDAKMEVNYLSLTSLNLY
                     VINEDMYDNNDKEISRIGDSYFLNSDKLLFVVPEYNGSFPGILKLLIDAMDVKKYLYF
                     KKAAIVGVATGRAGNLRGIDHLTSILQHTKITVMPHILPVSSVHLEIDEALEFKNQRT
                     KMALQHHLKQFLTF"
     gene            complement(9623..10726)
                     /locus_tag="PKY09_03480"
     CDS             complement(9623..10726)
                     /locus_tag="PKY09_03480"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11947.1"
                     /translation="MKIGNYIFELLHQHDCVIVPQFGGFVARYKNATIHPVQHLFSPP
                     SKIVAFNSDLTTNDGLLANYISQKLLLSYSDSCKLINDFVTLCKAQLEANQRIQIEKI
                     GVLFFDPEKNLQFIPADDENFLTDSFGLSTVQSPAIQRDRPFEIFSNRDAAVQPAPKR
                     TNHAKLILKIAAYTGVAAFIAFAYFNPAINSKITKGLATIFPVTTEIDSQANHALNNN
                     KQEQKSKEDLIYSESPVTEKNIYETEKPIADSSASQITGSTPEKEIATVEEEPMPQPV
                     IETKKLPESEPAVSSYTRQYHVVGGCFSVYDNALNLNTELKSKGFSSSLIGKNKNGLE
                     IVAIASVATKSEAEEALLQAQNSGYKDAWIMRK"
     gene            complement(10759..11805)
                     /locus_tag="PKY09_03485"
     CDS             complement(10759..11805)
                     /locus_tag="PKY09_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11948.1"
                     /translation="MKPTKQVLITFDYELFLGNRSGSVADCLINPTNQVLDVIEQHGC
                     RAIFFVDTTYLLTLKKNFKIAACKQDFETVMDHITEIASRGHEIFPHLHPHWLDANYL
                     SDTNEWQLINTDKYRFYHISEAERSEIFDQSIELLQQCVTKVNPSYLVDSYRAGGWCI
                     QPFNAFLPYFIKHNIKFDFSVLGGFYLFSNAQYFDFSKAPQKTIYQFENDITTEQNNG
                     RFTEFNISSIYIPQSIKLLEKLFLKLYYKITNDHSFSRGEGQIAVKIDKNLVTPQQQG
                     HDILDSAWERIAIELMSIIKQGEYKKYLNTNEYMHFISHPKMLTRHNIKMFDKFLKFA
                     SEKYNLETDFRKMV"
     gene            complement(11828..13000)
                     /locus_tag="PKY09_03490"
     CDS             complement(11828..13000)
                     /locus_tag="PKY09_03490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11949.1"
                     /translation="MHILIIPSWYKSITEPVLGTFFEEQARALMSAGHKVGIIYPQFS
                     SVSSMFNKKDEIIDFYDDNGLPTYSVIHQAIIPKMRKLAYKMFSENVEKIYYNYVKKY
                     GKPDLIHAHSVFHGGMAGYYIAKDNNLPFIITEHLTAYITGGITHQEDIETAKQIFNN
                     ADASIIVSENFKHDIEKVLNLPETTFTVVHNMVADLFFKDLIIKKFNQQEEFVFFTNS
                     FLLPRKNHKLLLDSIKYITAKGINNIRLRIGGDGPLYNELKDYVNELALTDKVDFLGA
                     LSRAEVKRELDHCHSFVLSSNYETFGVVLIESLACGRPIVTTDSGGPSDFIHSKNGIT
                     SLASIDNLANAMIKMMEEYNTFDQHKLSEECRSRFAEKKIEAEIEALYLKAIDKHK"
     gene            complement(13007..13960)
                     /locus_tag="PKY09_03495"
     CDS             complement(13007..13960)
                     /locus_tag="PKY09_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNB11950.1"
                     /translation="MNNDAPFQFLPGFTQLYHEYMEENIFIAYSEKLMAFMPLRFFSS
                     RFFKLAQILHAPIKNNIELNPQEQLDFFNELISYLNQNNSCERLVQPHPYGILASVPA
                     NSRFCEFGTYIIDLQTQTKEEIFQKFHPKYQKAIHHSEKNGAVVKFGQDVLNDFYLCY
                     TDTMKRISMPSEELQFFKSYYNYLGSDNVTAGVVYDNDNPIGGIFMIHSNYAALCTHA
                     GSRGETKLYGSMKYLHYKMIERMKESGVNKYDLVGVRIGNNDPALEGVFRFKKGFGGE
                     LKKGFLWKTDINPLKAKMYDLLIKIKHPNNKYKDIIDQITN"
     gene            complement(14015..15142)
                     /gene="wecB"
                     /locus_tag="PKY09_03500"
     CDS             complement(14015..15142)
                     /gene="wecB"
                     /locus_tag="PKY09_03500"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB11951.1"
                     /translation="MKKILIVVGTRPNFIKVTQFKKVNADIGMPFDIKIVHTGQHYDS
                     KMADIFFEQFDLTPDFWLNIPAGSPNSQMAEIMLRLEKVIQENKPDLLMVVGDVNSTF
                     AAALTANKMNLKIAHVESGLRSFDRTMPEEINRLLTDEITDYFFITEDSGYKNLIQEG
                     KQEKQMFFVGNTMIDTLVAFNEKIQQSNILETLNIKSKEYVLMTMHRPATVDHKDGLK
                     QLFSIIELITRNHQLVFPIHPRTLHRIEEFGMKSFITENKKLILIEPLDYLAFQKLIS
                     DCAYIVTDSGGIQEESTFRQIPCLTLRANTERPCTVDIGTNELVPFSNEKISERIDAI
                     VHNKYKKGVIPPLWDGQSTKRILEACMKFLSSERHASTLLK"
     gene            complement(15193..16335)
                     /locus_tag="PKY09_03505"
     CDS             complement(15193..16335)
                     /locus_tag="PKY09_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNB11952.1"
                     /translation="MIQRLISHIRLRYLNLLSQSTAENFSDEQRLLMALEWFKQSLLP
                     TGGSAAKYSMMFNKWLPAYPGTTAFWINTLIYLKNNKPELLHEVFNERPIVEELIQWL
                     LTTQRKDGTFPGSYGDFKNQPPKVFNNGQIIMALTDYYQQSQKKEVIQAAIQSADWLL
                     KVQDSDGAWREFTLHQLSSNTRTAWALIKLGQHLNESKYISAGINNINFAIKQTNKHD
                     YFCDNGFDENEIPTTHTIGYALRGVLGSAIDLQKQEWIEIVERSYKHIVALTKQNGFL
                     AGELDEEWTSDNAFCCLTGNCQLSVIGYILYEQTGETQYIETADRLLEYVKSKQLISH
                     SPMVNGGISGSWPVNGGYSAYDVPNWASKFFADAMLLQMKHKTKKQ"
     gene            complement(16345..18138)
                     /locus_tag="PKY09_03510"
     CDS             complement(16345..18138)
                     /locus_tag="PKY09_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB11953.1"
                     /translation="MSLSKNNYSTAQAFRQTYKSLQDHLSPLQKRRIKWIAFLIFLSA
                     ILDVFGLASVLPLVKMATEPEIIHTNTYLSTAYSYLNFETDKSFMVFSIVIILLFQFF
                     KTGFGIFVNYIEGRFMADVANNISKNQFSKYFSLSYFNFNSVKSSRIINHVQQNPASY
                     TAWVMLPILMLFSESIILLCIVTLIAVYNLKLFLFILMIIGPASYILYKSVNTKNEQI
                     GRGLDKMFPQALAAINNSIMGFVDIKLANKIEYYRNRFLKYQKAYHDLSMNSIVINMI
                     PFRGYELVAILGIVVIFIYAIFIEGGQQDVIMMVGAFAAAAYRLMPSLNRIMNAVMQV
                     TKNQVAIENLNEYNELYVKQKEMDRNIPIVFENTIEFQNISFTFPKSHEPVLKSISFK
                     VKKGEKIGFVGSSGSGKTTLMNILLRFFDENSGQILVDGKPLKEENLEYWRGLIGYVK
                     QDIFMIDSNIKENVAFGEETINSDLLNTAIKQASLNEFVGNLPKGVESEVGERGSRLS
                     GGQKQRIGIARSLYRNAQILVFDEATSALDTQTEREVSEAIDSLSDTNKTIFIIAHRV
                     TTLKNCDRIYELKNGEIAGVYSYSELIAKVI"
     gene            complement(18153..20462)
                     /locus_tag="PKY09_03515"
     CDS             complement(18153..20462)
                     /locus_tag="PKY09_03515"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNB11954.1"
                     /translation="MNNQKVIKNQSIIDAKDIKNALNLFVKNWYWFILFLAIGVAGGI
                     FIYYKTTRIYGAEAKVLITPTKNAFKDALSTSLPLGPNKEEVSNEIQILSSTKLVSEA
                     IKKLNLDISYYIKGRLKTGEVYKGTPFSVDGKILDDNFYGIPFNLRIIDKDSYSLDIK
                     TNEYSYNKVHKFGDPVVTDKFSIIINGKQESILKNPKFSEFQYVFVINNHGYLVKKYK
                     NALSLEKDEDASVININIADEVEEKAVDFLDTLTNLYINYSISVARGINENSLKFIEA
                     QLKDYEDQLNGVETSLVEYQQQSGAIDVANQQSTFLKEKIDVQGEKAKLTARLNSVDY
                     VYNQLTSGSSDFTTLSPALMTEQANPGLSNAFNELSALMQRKVNMSFSMTPNSPQIKE
                     VDAQINKAKEKVIGIIMNIRKDIVMQINLLTEREGNFAASLNRMPSTIKGLSDITRKK
                     EINEKMYLFLLESRAQNVIASAAIVPDKSILEPASSTGLLRPIRNKMLFMGAGVGLAL
                     ALAIVFLKSIFLNYIFTKEELADITNQPIIGVIGKSAEAKKDYLVVHSHPQSLAAEAF
                     RVIRTNLSYFAPKTSSKVILFTSTISGEGKTFCAVNTATMLARAKKKVVVVDLDLHKP
                     KQAVAWNMTNDVGVTSYIVGKASLKDTIKDTPVENLKVILSGPKSPNASELILDSMME
                     QLIIELKEHFDYIILDMPPVGLLSDALVMMKHSDLNLYVMKAGYSKKDFIEVAHQLME
                     KNQIKSLSFILNGVNPKNMPVGYGGAYYK"
     gene            20661..21776
                     /gene="gmd"
                     /locus_tag="PKY09_03520"
     CDS             20661..21776
                     /gene="gmd"
                     /locus_tag="PKY09_03520"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB11955.1"
                     /translation="MKRALITGVTGQDGAYLSELLLQKGYEVHGIKRRSSLFNTERID
                     HLYEDMHQKNVKFKLHYGDLTDSANIIRIIQEVQPDEIYNLGAMSHVRVSFDMPEYTA
                     NADGIGTLRILEAVRMLNMTEKCKIYQASTSELYGLVQEVPQRESTPFYPRSPYAVAK
                     LYAYWIIVNYREAYNMFASNGILFNHESPLRGETFVTRKITRAVSRIALGLQDTVYMG
                     NIDAKRDWGHAKDYVEGMWLMLQQDKADDFVLATGVTTTVRDFIVLAFNEVGIEIDFS
                     GKTENEIAVVKKCNNPAYQVPVGNVVMKIDPKYYRPSEVELLIGDPAKAKKVLGWEPK
                     HNLASIVKEMMNCDLELFKRDQYLKQGGHKIINYHEA"
     gene            21781..22722
                     /locus_tag="PKY09_03525"
     CDS             21781..22722
                     /locus_tag="PKY09_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNB11956.1"
                     /translation="MQKNSKIFVAGHRGMVGSAIVRKLQSEGFNNNLFRTSKELDLRN
                     QRDVADFFNQEKPEYVFLAAAKVGGIVANNTYRADFIYENLIIECNVIKSAFDSGVKK
                     LLFLGSSCIYPKMAPQPLKEEYLLTGPLEPTNEPYALAKIAGLKMCEAFRDQYGVDYI
                     SAMPTNLYGPNDNYDLKNSHVLPALVRKFYDAMENNLPSVEIWGTGSPRREFLHVDDL
                     ADACFFLMQNYSDRSTVNIGTGEDISIKELAMLIMETFGYKGQLNFDTTKPDGTPRKL
                     LSVDKLHQLGWKHSISLEQGVASVCNEVKSMESFLER"
     gene            22847..23713
                     /locus_tag="PKY09_03530"
     CDS             22847..23713
                     /locus_tag="PKY09_03530"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11957.1"
                     /translation="MKKFYLKFILFLAVSLCSKSNLIAQTQTVVIGTSSLISAFSPIV
                     KSYECVAYESIYLSTEIGYSGAISTFGFNRVDGTDTLPFDSVSIFMKQTALTSLTAGV
                     IDTTGYTRVYDGSFPNSAGAGWREVSLNLPFAYSNSSNLSVLVFKHTQAATAGTPVTP
                     RWYYTSVSPNRARRYYGTSPMAWGPNLSVTNVLSDIRMTIGTVGIVDISAGLCSVYPN
                     PTDGLVNIVVHNEKVASMLVSDISGKIVADVTTLKQLTVDLRNLSNGIYTIQFFDNTK
                     QLMAAKKIAIKK"
     gene            complement(23753..25489)
                     /locus_tag="PKY09_03535"
     CDS             complement(23753..25489)
                     /locus_tag="PKY09_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNB11958.1"
                     /translation="MQNTFKLFMAILFLCSTVSAQENLPNYMTQKEVLQMPSYLQQFS
                     NNLKNNQVAAPPSSRVRTMAEWEELQGILIGWTSYPAILREIVRAAKTQCRVYIVTNN
                     QQSVINYLNSGNVDTINVSFVNTPFNSVWSRDYGPWSAYTNDVDTLVTIDWIYNRPRP
                     DDDNVPVAIAGLLNTPLYQTTMSPYSLVHTGGNFMCDGFGTGFSSNLIVNENPSLTTS
                     QIDTIMKQFMGIERYIKMPTLPYDGIHHIDMHMKLLNEETILMGQYPQGVADGPQIEA
                     NLLYVVNNFLSVFGTPYKVIRIPMPADNGAYPNTTGDYFTYTNSSFINNTIIVPTYNV
                     SQDSTALRIYRDALPGYNVIGINSLQSIGALGALHCITKDLGTNDPLLISHQQLADTY
                     DTINPYSVVAFIKHRSGIQSATLYYRTDTLQPYNAVPMTALTGMSDYFETYIPAQSGG
                     TRVYYYIEAYSNTSKHQTRPMPAPLGYFYFDILQTTKINALDNPITISKIFPNPASAI
                     TCLPYSSHIGTMFQIKINDVTGKLVYESQNTSKSNGQNFAFFDVSNFKPGVYFVKFET
                     NSSINIQKIIVK"
     gene            25595..26446
                     /locus_tag="PKY09_03540"
     CDS             25595..26446
                     /locus_tag="PKY09_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNB11959.1"
                     /translation="MKWMGRRESDNVEDRRRFSGGKIALGGGLVGIIVIVINLLMGGD
                     PNQLLQQMQQQATTQTTSQETSAADDEMASFIKVALADNEDIWKKIFQENGIEYREPK
                     LVLFRDATQSGCGNASSAVGPFYCPADEKVYIDLSFFDELKSRFGAQGGDFAIAYVLA
                     HEVGHHVQHLLGTSDKVHEMQNQLSETEANKLSVALELQADFYAGVWANQNQKINNVL
                     EDGDIEEALSAASAVGDDKIQKKAQGYVVPDAFTHGTSEQRMFWFKKGYQTGKLQEGN
                     TFKELNI"
     gene            26551..27399
                     /locus_tag="PKY09_03545"
     CDS             26551..27399
                     /locus_tag="PKY09_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNB11960.1"
                     /translation="MQKFTTSLMALIIATTTTSMAQLKTPQPSPTQSLTQAFGLGEIK
                     IEYSRPSVKGRTIFGDLVPFGKIWRTGANQSTKVTFGADTKVNGNDIAAGTYALYTIP
                     NTADWEIIFYKDLTLGGNVGDYKKEDEMMRFKVKPMATADKMETFTINVNNIAPTSCS
                     IDLLWDKTKVSIPVTTNIDENVMKQIDKAMGDNKPYFQAANYYYENDKDLNKAREWIN
                     KAVENNPKAYWVLHTKAKIEYKLKNYPEATKAAEQSLALAKESSDNSYIKMNEKLIAD
                     VKKMGK"
     gene            27651..27947
                     /locus_tag="PKY09_03550"
     CDS             27651..27947
                     /locus_tag="PKY09_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11961.1"
                     /translation="MRKLVKRLSALSAMLIISTVAFSQSTTTQETEQTTTPPTETTES
                     TEDSTGAVEALPDSTAVAPEGDPAAGAANEKPATRHKLYSGKQRGNNVIYDPKK"
     gene            28003..28479
                     /locus_tag="PKY09_03555"
     CDS             28003..28479
                     /locus_tag="PKY09_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11962.1"
                     /translation="MRKLLMSSAICSVLLLSTMLSFGQQAEKKSTESGFFVRNGLVYY
                     IKGNTVNFLESDQTLDNGATINQRGMIKYLDGKTAQLQQGDIFTFNNVLVPKAVEEHL
                     ELKDGKLQIVTNGIFTPVEKSMKLETGVTIDAEGHTTSGITLMPKQKMNMMGELIK"
     gene            28546..29352
                     /locus_tag="PKY09_03560"
     CDS             28546..29352
                     /locus_tag="PKY09_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNB11963.1"
                     /translation="MFKFIDQTGFEIKLEAPPQRIVSLVPSQTELLADLGLQDSIVGI
                     TKYCIYPPELFLSVPRIGGTKSVNHELVSQLKPDLIIANKEENNRDSIEQLREQFPVW
                     LSDVSNLHEALDMIYNIGVMTNSQERASEINNQIYDKFNSLTLKNKKHVAKRALYFIW
                     RNPYMCAGGDTFINDMMNKAGFDNLMYYHKRYPELTEKRISELNPEVILLSSEPYPFK
                     AKHIQEFKSICPKAEILLVDGEFFAWYGSRMIKAADYFYQLCDSNSNVSE"
     gene            29365..31653
                     /locus_tag="PKY09_03565"
     CDS             29365..31653
                     /locus_tag="PKY09_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB11964.1"
                     /translation="MMQKWIKLLSIISLLNVNFLFAQKVGLVLSGGGARGFAHIGVIK
                     ALEENGIPIDYITGTSAGALVGGMYVSGTSPQQMQNIVLSEDFKNWATGSLSDELIYY
                     FKERERDASWATVKLSYDSTLRMNFNGSLVNSGQIDFALLENHSAASAGADYDFNNLF
                     VPFRCVASDIKNKRPVIFNCGDLSQAIRASIAYPLYISPMTADNQLYYDGGIYNNFPS
                     DVMLNEFDPDIMIGVSVGGGPDIPKEDNMFSLLRTMIIQSQHVVLPGADDILIQPDVT
                     NFGVFDFYNFQSLIDSGYKATMARMDDIKQAIARRISPEELSRKRDDFIKKLHPLVVG
                     DIIVTGVSPEKAKYIKSILNPRGSNMLLRQLKPRYFRLLADENIRYAFPKLVYDPKTE
                     VYNLEVYIKPETDLRVDIGGNFSSRPISEGFIGFRYNVLGPYSLGIHANTYFGKLYNS
                     GQLKLRLDLPGRPDFFIEPFATINRMDYYKSSSFFKDDIKPAYLLVTDQSFGLNIGMP
                     ARNKGALIVGGNYFNIKDRYYQTRNFSVNDTADFTRFEGVSCVVGFERNTLNRKMYAS
                     KGSYLSIRTRFVSGNEITNPGSKQDNAPTISEWHQWWQVKMIYDNYFFRAGKYKLGFY
                     AETNFSGQPFFNNYTATILQMPGFYPTQESRTLFLDNFHAPNYLGIGVRNVFSLRSNI
                     DFRLEGYGFQPFQEVVKNENEKAVFGDALSARYFIASFSGIFHSPVGPVAMTLNYYDR
                     KSKSFTVLFHIGYILFNKHALD"
     gene            31680..32750
                     /locus_tag="PKY09_03570"
     CDS             31680..32750
                     /locus_tag="PKY09_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB11965.1"
                     /translation="MSKTVIVAPLNWGLGHATRSKTVIDNLLTAGCKVIIASDGNSLK
                     ILKRQFPFLHTEELPSYSPVYPSDGNMAWAMLKQAPKMLINIGNEQSVIKKIATKYNA
                     EIIISDNRYGCFTNFTKNIFLSHQLMIQSPESMLFLQPSINRLHAKFINRFHECWIPD
                     VVQGFKLAGTLSENKYIKIPKFYCGLLSSYLSSNMSNDNKFDIVAILSGPEPQRTIFE
                     GRIIEQLEKIRYRSLLIRGKISDPNEIPDRGVMQFKNYVSAEGISNYISPNTFVISRS
                     GYSTIMDIAQLGVSALLVPTPGQTEQEYLASYFDENKIFMMQTQNNIDIQKAFERKSD
                     YLAIKTEKNILLQQNLNRILLT"
     gene            complement(32747..33001)
                     /locus_tag="PKY09_03575"
     CDS             complement(32747..33001)
                     /locus_tag="PKY09_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11966.1"
                     /translation="MNNPQLKASEKFPATINELYDEEGELVMILYAHELVDLPEGLWL
                     STEGYYEAVIKKDEIQIEDALFCISELNSITGMQYELALS"
     gene            complement(33010..33783)
                     /locus_tag="PKY09_03580"
     CDS             complement(33010..33783)
                     /locus_tag="PKY09_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB11967.1"
                     /translation="MSFGICEMSLVPMRKEPSHRSELVSQILFGEHYEVVESQKEWFK
                     IRTSHDGYEGFIALNQHTEISYNDFQFLQKNGAFVCFDLVQLLLHDNKIQTVILGSAL
                     PFFENQHCRINTRTYRFDGNAKFPQKAVTSALLIENAYMYLNAPYLWGGRSPFGIDCS
                     GFTQLVYKLSGYSLLRDASLQSEQGNIVNLVDEAMPGDLAFFDNEEGNIIHVGILTSR
                     DTIIHASGKVRIDAFDQVGIYNQETKRYTHRLRLIKRIF"
     gene            complement(33803..35164)
                     /locus_tag="PKY09_03585"
     CDS             complement(33803..35164)
                     /locus_tag="PKY09_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNB11968.1"
                     /translation="MYFNNILETIGNTPLVKLNAVTQGVQATVLAKIETVNPGNSIKD
                     RMALKMIEDAEKSGKLKPGGTIIEGTSGNTGMGLAIAAIVKGYKCIFTTTDKQSKEKV
                     DALRAFGAEVIVCPTNVEPEDPRSYYSVAARLSKEIPNAFYPNQYDNLSNRQAHYEQT
                     GPEIWEQTEGKITHLVVGAGTGGTITGTGRYLKEKNPKIKVWAIDTYGSALKKYHETG
                     ILDKNEIYPYVTEGIGEDFIPQNYDFTVIDHFEKVTDKDAALMTRDLVRKEGIWVGNS
                     AGSAVAGLLQMKDQLKPTDVVVIIFHDHGSRYIAKIFNDDWMRSKGYFDKKGMTASDI
                     IGTKKDVNLTTVETTLPIGDALKIIKTNNFSQLPVTREGRIVGNITENHIYSSLLKSP
                     ELKNKPVSDIMQDAMPFIDINTSIDTLSDLLSKNNQAVIVKDFKADRNYIVTRQDVVD
                     ALA"
     gene            complement(35199..35747)
                     /locus_tag="PKY09_03590"
     CDS             complement(35199..35747)
                     /locus_tag="PKY09_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11969.1"
                     /translation="MNNILKDNKTKFITISILIVAISRLLPHPYNFTPIGAMALFGGT
                     FLKDKKIAFGLPLAALLISDLLLQLIKPGSAFYEGWFWIYLSFILITPLGFLLRNKLK
                     VQNVVAASLMGSILFFIMTNFGVWTTGLMYPLNIAGLMSCYVSAVPFFGGTIMGDLFF
                     NMLLFGVFAIAKWRMPALVKEA"
     gene            complement(35861..36586)
                     /locus_tag="PKY09_03595"
     CDS             complement(35861..36586)
                     /locus_tag="PKY09_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNB11970.1"
                     /translation="MRNFGLIGFPLSHSFSQIFFTDKFKKLNISCNYNNYEIGDLSNL
                     KALISNNQLSGLNVTSPFKTSIIPYLDQLEETGKTVGSVNTILIKDEKLIGYNTDIIG
                     FKISLERIIGDQKPPTLILGTGGSAKAVAFVLKQKSIAFKFVSRNANESAISYLQSDD
                     IISQNLLIINCTPMGRFPQIAQKPPINYSKLTDKHILFDLNYNPEITAFMNEGIRRRC
                     KVCNGYDMLIEQAEASWTIWNYN"
     gene            complement(36583..37227)
                     /locus_tag="PKY09_03600"
     CDS             complement(36583..37227)
                     /locus_tag="PKY09_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNB11971.1"
                     /translation="MEHLKFLIDFILHIDKHLFELVQRYDTWIYLILFLIVFAETGLV
                     VTPLLPGDSLLFAAGALAAPPDNPLNVFYLMIIFFFAAFGGDNTNYFIGNYIGPKVFE
                     KNYRFLKKDYLNKTQEFYEKHGGKAVIFARFVPIVRTFAPFVAGVGSMTYNRFIGFSV
                     LASVLWINICVWAGYFFGRLEIVKNNFSLVVIAIIGISLIPILIGIIKQQFSSK"
     gene            complement(37237..37554)
                     /locus_tag="PKY09_03605"
     CDS             complement(37237..37554)
                     /locus_tag="PKY09_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB11972.1"
                     /translation="MQEITVHELKQWQDEGENFQLIDIREEYEYDACNLNGMLIPMGE
                     IIDRIEEIAKDKPVVIHCRSGSRSAAIYQVLTTQYGFTNIFNLKGGIKAYSQEIDPTL
                     NVI"
     gene            complement(37600..39315)
                     /locus_tag="PKY09_03610"
     CDS             complement(37600..39315)
                     /locus_tag="PKY09_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB11973.1"
                     /translation="MSIIDWLVLAITLLAIVIYGIYRSRHKKTLDSYLIANRELPWYN
                     VCLSVMATQASAITFLSAPGQAYSDGMRFVQFYFGLPLAMIFLSATFIPAFRKFNIYT
                     AYEFLERRFDHKTRKLTALLFLLQRGVSTGISIYAPSIVLSTVLNIPIFYTTMIIGVL
                     VIIYTFYGGTLAVSHSQVLQMTIIFSSIIIAGVIVLSLIGTDATLYESLKIAGIHNRM
                     NIIDFKFDWNNRYNVWSGIIGGFFLQLSYFGTDQSQVGRYLTGKDSSASKLGLIVNGM
                     VKIPMQFVILFIGILVFSFYQFQAPPIFFNENIEKIAKESPMADYYNSLEFRFNNINE
                     SNSNLSKDYLKYLRSNDLANAEFTKTKILQTSAEANEVKQEAVKIIKTINPEADINDT
                     NFVFLHFVMHHFPKGLIGLIIAIIFLASMGSLAAGINSLTSTSVVDFYFRSSKKEYSE
                     TKKLYYSKIATLLWGIFCIICAFFAGNTGNLIEAVNILGSLFYGTILGIFISAIFVKQ
                     LDGNSVFIAALITEAIIITFWYFDVMAFLWLNLAGCLLVVFIAFIIQFSKQNLNRMFN
                     KPISK"
     gene            complement(39416..40555)
                     /locus_tag="PKY09_03615"
     CDS             complement(39416..40555)
                     /locus_tag="PKY09_03615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB11974.1"
                     /translation="MQLSFWEKSQWFDNVDIVVLGSGIVGLHAAYYIKKKNPRLNILI
                     IERGFLPYGASTRNAGFACIGSASEILNDLDKDSEYKVFQLVEQRYKGLLGLRELLGD
                     ENIEYEQNSGFEIFTETDSQSFLECESKLEYLNTRLKSITKDNTYKIVNERIAHFGLS
                     KVKNLIQNKFEGQIDTGKMMFVLSEKVRQSGVKILNGLEIKQIHTESSGVTLMSSEGF
                     QIKAGKVVICTNGFAKEFLSDISITPGRAQVLITNEIKDLKLCGSFHYQSGYYYFRNV
                     GKRVLIGGGRNLNFQGETTTNFGVTEQIQASIEKLLEDIILSGVEYKIEQRWSGIMGL
                     GESKSPIIQEIKPNIYCAVRLGGMGVALGYLVGKEVAELVSLNYQ"
     gene            complement(40582..41151)
                     /locus_tag="PKY09_03620"
     CDS             complement(40582..41151)
                     /locus_tag="PKY09_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11975.1"
                     /translation="MIKKSILYFLLICFPKILSAQTDTIPPIRENKSKIKSDKIPFKD
                     RIYFGGNLGLQFGTQTYIDISPLAGYKFTETVSAGAGITYIYYKYKDSYYTYSTSIYG
                     GRIFGRYLFTNNLFAHAEVELLNMELYDLNINDYYRKNIISPFVGGGYIQRFSNNAGL
                     YLLLLFNLNDSAESPYTNPIIRIGFNLGI"
     gene            41208..41897
                     /locus_tag="PKY09_03625"
     CDS             41208..41897
                     /locus_tag="PKY09_03625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB11976.1"
                     /translation="MNIVVTGASSGIGLELVKSFTLQNHNVLAVSRSKEKLTELKRFS
                     ESIHILSIDINRLNSEVITETLIKSKMKHIDILINNAGVLVKKPFVELTESDWLETYN
                     TNVFGVANMIQCCMPYILKSQIRHIVNISSMGGVGGTLKFPGLSAYSSSKGAVTILTE
                     CLAEEFKSDAIKVNGLALGAVQTPMLEKAFPELKTFQTPENIVPFISWFALEAHKWCN
                     GKIMQVSDSTP"
     gene            complement(41914..43701)
                     /locus_tag="PKY09_03630"
     CDS             complement(41914..43701)
                     /locus_tag="PKY09_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11977.1"
                     /translation="MKMTFSLIFCVISLLFFNNLFGQINSFDNKYYGSTPLTIENNFI
                     KSLKKYNSPVIDFVEFNQSPIETTCFIQKLGTNEKISEFKSTKKALSHLEFETASKKY
                     NSLFVQHNQKLSPSLVGFVNFNTLKTDGYYLRTAYKKIFVEVGAFNFDTLNRVNYSFH
                     VLNNKIKSNESGGLSSDSLFIFKEQNSKFFTTNLATSSSTRKLSEFGADLNIRLFNTG
                     LQKNIDSLNKTQDWNLRWLNSFVYSELKYWFESDNKEDFYQNHYIDTTSTDDLFVYKP
                     LTYSSKLELNKNYSALKININGHLGYNGLLNKVSQNGIDSTYYVSNLFWGGGIQNTNK
                     YGLFYIGEFAFLRNQDAYNHSNHSILFYYLPISKWRIDGKIESKKQMPSYIENRFYSN
                     HYKWKNQFGLVNYKSFDLTSSIIDSILVIKYSLTNLKNHIYFDYSASPKQFSAAININ
                     RLTLYTNIQVKKFHITNYFIYSSITNNDIYRFPKIILGSRVCYTQYFKNNKIAVSPQI
                     SLNYLSSFQSYSYEPASGSFYLKDGYKKSGNYPFFDFSFEVKMSNAVIEIGCNHINKG
                     LSGRNYFLTTSYPTPERSIYLKVIWSLVN"
     gene            complement(43814..43925)
                     /gene="rrf"
                     /locus_tag="PKY09_03635"
     rRNA            complement(43814..43925)
                     /gene="rrf"
                     /locus_tag="PKY09_03635"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(43980..>44292)
                     /locus_tag="PKY09_03640"
     rRNA            complement(43980..>44292)
                     /locus_tag="PKY09_03640"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT        14430 a         7429 c         7875 g        14558 t
ORIGIN      
        1 aaaattcaaa gcattaaatg ataaacttgc aaaacacttt tccattgttt tcaaaaaaac
       61 tcatgaatgc tatgtttata atgccattgg tatttctgat gttgtttgtg tccaatgttt
      121 gggctggagg caacgataca cttgctgcta atgttgatga aaatgttcac gttgcagaga
      181 agcctaaaga ttttgaaata gcgttgcaac ctgctttact cagtgataac agcacttcac
      241 aaattcgtgc agggttgatt tatgattgca caacaaatac aattgtctgg gaaaaagatt
      301 taaagtatgc ttatccaata gcgtcactta ctaaaatgat ggtggcacta attgccattg
      361 aagaaattaa gtgtggtact gttgattggc aagatcaggt ttctgtgaca aaaacttaca
      421 gaaaaagtaa aagaagtaag cgtacttata cagttaacga aacatatagt cttgagtcat
      481 tattaaaaat ggctatgatc ccttcaaata atgaagcttg cggacttata ggaaaacata
      541 tcagtggctc tgttgaaacc ttcgtgacaa gaatgaatca gaaagcagca gcattaggaa
      601 tgacacaaac tttctattca aatccatccg gtttaccggc aggctatgga agcttagaca
      661 acagttcttc accttacgat ttgttaaagc tgtcaacgga gttaatcaaa tttcctgaac
      721 ttctttccat taccaatatt gcttttgcag aggtagaaaa cggccatgga tccaatgttt
      781 acagaaacca taaccatttg gttatggact atcccggaga agtggacggg ctaaaaaccg
      841 gctatacaaa aaatgcaaag ttctgcttgg ttgctacagc aaagaaaaac gatcatcgtt
      901 taattgccat tgccttaggt gcatcaaacc cttggttgcg caatcagatt gttggtgaaa
      961 tgctcaataa ttattacacg cagttgggtt ttggacctat ggggaatcaa aaagttgatc
     1021 cattgtttgc taaggcagat agaaaaacag taaatgatat tgcaagcagc aatgtacctg
     1081 taatgaatca ggaaatagtt tataaaacag ttacatcaat tcagaaaaga gcacacattg
     1141 taaaatcagg acaaacctta tcagacattg ctgataaata taattgttct gttaatgaac
     1201 ttaaaaaatg gaatgggcta aaatcaagtc ggattcttaa aaatcaaagg ctttatgtac
     1261 gtgtacacgt gaagaaacaa attcctgtca gtacaaaagt tattgataat tacgatgaat
     1321 gtgaagacaa tgcagaagat tgcgcaccac aaaatgttgc cgataagcag gaaactccaa
     1381 agaaagaaca actgccaact tcgcaaagta aacctgcatt gaaagtgata tatcatgaag
     1441 tgcagcccgg tgatacttta tggaaaattg cacagcaata tccgggcact tctgttgatc
     1501 aactgaaaaa aataaataaa atttcaaatt caaaagacct taaagcaggc agtaaaataa
     1561 aagtgtcagt aaaaggctga tatttaaaca aaccaaatca gaatgaaaat caaactttta
     1621 gttacaagtt taattgtggt atttagtgtt ggccggataa attctcagac agtaagctat
     1681 cccgttgaca atgattcaaa attaatttgc tacaccaagg taaaagagtt agccggagtt
     1741 tctaagaatg atatatttga cagagcatta ggatggatta attcctatta caagaaccct
     1801 gctgatgtgt tgcgtgaaaa tgataaagcc aatggaaaac tactttgtaa agcacgattt
     1861 aaattaatgg gcgagcctga taagagtgga tttcaaaaag atcagggcaa tgtgcagtat
     1921 tcattgatta ttgaagcaaa ggaaggcaag tacagatata aactaacaga aattaactgg
     1981 aagcaacctt cttatttccc tgtagaacgt tggatggata caaagaatca atactacact
     2041 aaagactggg attggaattt gaaatatacc gatgagcaat cgcataaaat cattgctgaa
     2101 ttagagaaat atatgtcaaa tccacctaag gtcaagaagg acgattggta acctaagcta
     2161 ctctttttga ctgactgctt tccgtaattt tctgattttg caaatcaaca ttttcaatta
     2221 aaaccaaacc gggttttttg cctttttcta aagtgccaag ttcgctgtcc attttaagag
     2281 cctttgcacc attaattgtt gaccaggtta aaagttgatg aagtgatatt tgagggaatt
     2341 tttttgtaat gatttttaat tctgataaaa tactcaactg attgtttgaa gcaagactat
     2401 cagtgccaat caagatgttt tcagttgctt tagttattcg ttcaatttcg ggaagggtat
     2461 tttcaatata taaattggca ttcggacaaa gacaccacca gcatttttta gtatttgcta
     2521 ttgcatatcg tatgtcatca tcactgctga aagtattatg gacaaaaatg atttcatcac
     2581 aaaggttcaa ccatggcaca tagttttgaa ttgaactttt tccgtttggt ttaatatgcg
     2641 gcaatttatt tcctgacaat tcaaagaaat tttttaaatc acctgtgccg gtttcgaaaa
     2701 gcgtgttttc agatgccgtc tcctgattgt gtatggttat tattgaaggt ttattggcgt
     2761 ttattttctg aaacaaatgt ggtgttacag agtagggtgc atgtgggctt atgctgcatt
     2821 ttgaatttct gttgtttaac tgtagcgttt ctttaagttc tttagccatg ttccacactt
     2881 tatctgtatt caattcaaac aagtcataag cttcaataaa agtgtggtat tgtaaatcag
     2941 atttattctt taattgaaat gtaatggagg tattgctgat atcgccaacg gcaacaatac
     3001 ccacttctaa catactttct tcagccgatt taatagtgtt tattatttta tcgtgctcgg
     3061 ccttcattct aacagagacc aaatctttaa taaaaccggt gagtttgttg tgtttattta
     3121 tttccccttt aaggtacgag agttcaagat gacaatgtgc gttaatgaat cccggtgtca
     3181 acataccatg aaatttttga atggcagacc tttctatttg aatttgcaaa gcattattca
     3241 gtgatgcaat attttcaaaa acatcaataa ctactcctga gttgtccatt acaacaatgc
     3301 cgtctttaag gggagtactg caaacaggga aaacaaaatc tgccgaataa aatttcataa
     3361 ttacaaaact aaatgatttg tgtatcggct gatattttca tcgtaccttt gtgaagtgaa
     3421 atcaacacct aacatcaggg aactcacttt tgatcaactg tctgaagcca ttgctgagtt
     3481 tggcgaaaag tcattcaggg ctaaacagat atacgactgg ctctggaaca aaagtgcacg
     3541 ttcatttgac caaatgaaca atgtgccatt gtcactccga aataaactgg ccgaaaagtt
     3601 tgcatttact cctatttgtg tagcacatga acaacgcagt aatgacagaa ccattaagta
     3661 tggatttaaa ctacatgatg gtcatttaat cgagggtgta ttaatcccca cagcttcacg
     3721 aatgactgct tgtgtttcct ctcaggttgg atgcagtctg tcatgtaaat tttgtgcaac
     3781 aggcaaattg gatagggtgc gtaatctgaa tgccgatgag atttacgatc aggtggctat
     3841 tatcagagaa caaagtatgg cggtttataa tattccctta agcaacatcg tatatatggg
     3901 aatgggtgag ccactattaa attatgccaa tgtgctgtca tccattgaga gaattaccgc
     3961 tgccaatgga cttggtatgt cacctcagag aattacagtc tcaacagccg gaatagcaaa
     4021 gatgataact aaacttggtg atgatgaagt gaagtttaat ctggcgcttt cacttcatgc
     4081 tgctaatgac agtaagcgcg ataaaattat gcctatcaat gaacataata atctggaggt
     4141 attaagcgat gcactcaagt atttttatga caaaacaggc actcgggtaa cctatgagtt
     4201 tattgtcttt aacaacttta acgactcgct taaagatgca caagaactgg ctgaatttgc
     4261 caaacatgtt ccttgtaaga ttaatcttat agaatacaat gctataggcg atggtgtttt
     4321 tgaaaaagcc cgattggaca ggttagaagt atttaaaaat tatttggaaa gtaaaaattt
     4381 ggtagtcaat atcagacgca gcagaggcaa agatattgat gctgcatgtg gtcaattagc
     4441 taataagaat cagtaaactc ataaaggctg ctgctactta aaaacctacc tttgcagccg
     4501 ttaattatga cgctcagttt agatcagatt aaaaaaccca tttcgcttga aatggacagt
     4561 tttgaaaaca agtttcgcga tgccatgcga agcaaggtgt cattggttga caaaattatg
     4621 cactacattg taaagcgcaa aggcaagcaa atgcgaccca tgtttgtttt tctttcagcc
     4681 ggtgtgtgtg gcaaaataaa tgaatctaca tatacagcag catcccttat tgaattgttg
     4741 cacactgcaa cattagttca tgacgatgtt gtggacgatg ccttacaacg aagaggattt
     4801 ttttctatca atgcagtctg gaaaaataaa attgccgtgc tggtgggcga ttatttattg
     4861 tcacgtgggt tgcttattgc tgttcagcat aaggaattca aacttttgga aattgtgagc
     4921 aatgcagtgc gtgatatgag tgaaggtgaa ttgctgcaaa tggagaaggc tagaagactc
     4981 gatattgatg aggatgttta ctataagatt attcgtcaga agacagcatc acttattgca
     5041 tcttgctgta gttgtggtgc agcatctgta caggcagatg atgacaggat tgaacttatg
     5101 cgcgaattgg gagaaacaat aggattagca tttcagatta aagacgacct ttttgatttt
     5161 gaaaaaagta tctctacggg taagccttca ggaattgata ttcgtgaaag aaaacttaca
     5221 ctgcctgtta tttatgcctt atcacaggca tcatcagtag aaaaaagaac aatcatcaac
     5281 ctgattaaaa atgatcatga aaatgaaagt aaggttgctg aagttttagc gttcgtcaat
     5341 aagcaaaaag gtattgatta tgccagagaa aaaatgtttg aactcaggaa tcatgctttt
     5401 gaaattatca atagttttcc tgatagtgat taccgaaagt cattgcatga tctgattact
     5461 tttacaacag atcgcaatta ctaagtgatg ttaaagaaag gaattattgc aagtttaatt
     5521 tgtattggca gtttactttt actcctgtac tttacatcgt tatttagtat cagtgaatta
     5581 atttacccga atagggtaac agttaaatct ttgacttcgg aatttcataa cctgaaaaag
     5641 aacgaattgg tcatagataa agatataaca gtatctcctt cacaactaaa tcttacttat
     5701 aggaattttg atgtgttgat tcatgatcaa tttacattac atggttggta tattacaggt
     5761 gagcagcctg caaaaacaac agtactgttg ttgcatgatt ggaatgaaag taagttgagt
     5821 atgataaaag ctgcagaggc tttgtatcag cttggattta atatttgtat aattgatttg
     5881 ccggcacatg gcgagagcga tggtgaaaaa tttattattg accagtcaat gaaccattgt
     5941 ttggaagtgg ttgctgacag tttattttgc ttatatgaaa cggataacct tgcagtagtt
     6001 gcaaacgggc tttcgggaat tgtagctgct tcctggacca aggtggatgg ccgcaatcaa
     6061 tgcctgattc ttgttgatcc tgtgccgggt atgactgcta aaatcaatga attggttaag
     6121 gaaaaatgga atggattatc tatgttaata agtcaatttg cacaaataat gtaccatcaa
     6181 aagacgggta tagatgcaga ttcattaaat ttgcaagagt atttaggaca gttaagaaca
     6241 ccgcttttag tggctatttc tgatagtgca acagatatag aaataattga gtcagttcgt
     6301 atttttgaga gtgcaaaatc acctaataag aaaatttgga ccaaccaaac aagaagtttc
     6361 atcaggatag atacagatgc agaaaagaat atgttcaggg caatggcagc atttattaac
     6421 tcaaatattt cttcagataa gccaaaaata aaagtgagaa aaagaattgc cgcattatga
     6481 taccaaagga aaccgttgag ttgattatag aaacttcccg aatagaggag gtggttggtg
     6541 attttgtttc actaaaaaaa cgtggtgcca acttacttgg tttgtgtcca tttcataatg
     6601 agaaaactcc ttcgttcaca gtatcgcctg caaagggaat atacaaatgt tttggttgtg
     6661 gaaaagctgg cgactcggtg cggtttatga tggagcatga ggcttttact tatcctgaag
     6721 cattgcgtta tcttgcagaa aagtataaga ttactattga ggaaacaatc cccactgttg
     6781 aggaaattca aaaacaagat gagagagaaa gtttgtttgt cttgtattca tttgcacaga
     6841 aatttttttc ggaaaaactc ttcaatacag aagaggggag agccatcggg ctttcatatt
     6901 ttaatgagcg tggttttaat gaagcagtaa ttgaaaaatt tcaattgggc tatgctcccg
     6961 atcaatggga tgctttgtct caggctgtaa cagagaataa gtttaacagg caattatatg
     7021 agaaaagcgg acttgctgtt gtcaaagagc aagaacagga tagtcagaaa gtttacgatc
     7081 gctttcgggg aagggtaatt tttccgattc ataatctttc aggacgagtt attgcatttg
     7141 gtgcaagaca gttggcgaat gatcccaaaa gcccaaagta tattaattct cccgagaccg
     7201 atatttatca caagagcaaa tcactttacg gaatttcctt tgcacgcaaa tccattattc
     7261 aacatgatga gtgttttctt gtggaaggct atactgatgt tgtttctcta tttcaggcag
     7321 gagtagaaaa tgttgttgct tcttcaggta cttctttaac tcctgaacaa ataagactta
     7381 ttgcacggtt tacaggcaac ataacaattt tgtatgatgg cgaccctgca ggaattaaag
     7441 catcattgcg tggcattgat ctcattcttg aggaaggcct taatgtaaaa gttgttttat
     7501 tccccgatgg tgatgatccg gattcatttg cccgtaaaaa cagtaccata cagttgatag
     7561 actatatcag taaaaatgct gttgatttta ttggttttaa aacaagcctg cttttagaag
     7621 aagtagctaa tgaccctgtg cgcaaggcag cactgattcg tgatattgtt gaaagtattt
     7681 ctcgaattcc cgatggaata aaaagaagcc tatacacgag tcagtgtgca acacaaatgg
     7741 atgttcctga gcaggtttta attactgagc tgaataaaat cagaagaaac ttatacaaaa
     7801 aagataatga acatcagcag gcagaattag ttgaaaagtt gattgaaatg gagtatttgc
     7861 ctcagcaaac cattttgact gaaacaaatg catggtatca ggagcaggaa attattcgtt
     7921 tactattgaa ttatggggat aagaatttta attatcaggg tggagaacag caaaagacag
     7981 ttcgtgagtt tattctggag cagattttac ctgatggctt tggttttgat gatgaaattt
     8041 ttgcaggagt tttcagttac ttaatgtcta tgagcacagc agatttctta tctatgctta
     8101 aaactgagaa tagcagtgat ttggaaaaac tccgttcaaa gacccacgaa ttgctgatga
     8161 ataaatatga attaagtgat tgggaatcca aggggatata tgtaaagacg gaagaaaata
     8221 attttaatga ggccattatg aatccgataa accatatcaa gttaaaacgt gtgcagatac
     8281 taaaaaggaa aaacagtttg ctcttgaaag aagaatcgaa tattagtgat gaaaggatgg
     8341 atgagatttt atccaagcaa aaagtttacg aaacatatat taatgcactt tctaaattac
     8401 tcggaatagt tacacttgaa taattttgca gaatgataac cctaattgca ggaacaaata
     8461 ataagaacag tcacacagag aaattagcac gtatttatta tcaggcttta ttggatgcaa
     8521 aaatggaggt taattatctt tcgcttactt cgctcaatct atacgtcatc aatgaagata
     8581 tgtatgataa taatgacaaa gaaattagtc gaataggaga tagttatttt ttaaattcag
     8641 ataaattact ttttgttgta cctgagtata atggaagctt tcctggtatt ctcaaactgc
     8701 taattgatgc aatggacgtt aaaaaatatc tatacttcaa aaaagcagcc atagttggcg
     8761 tagctacagg gcgtgcaggt aacttaaggg ggattgatca tttaacttct attttacaac
     8821 atacaaaaat caccgtaatg cctcatattt tgcctgtttc atctgtgcat cttgaaatag
     8881 atgaagcgct agagttcaag aatcaaagaa caaaaatggc actgcaacat catttaaagc
     8941 agtttttgac attttaggac atatcagtaa ggaggtttag tgtctgtttt cattttatcc
     9001 taaaaaaaat caaaaattga tgcattattt gcatttcatc ctaaaagcct tttagatatt
     9061 attcgatgaa tataatattg tattaacctt ttgaaagtag agaattgaat aagtctccag
     9121 ataatatatt ccatggtttt atcagccggg taactaaaac tgaattatga aaagccgacc
     9181 taaaaataag tcaatataaa aaggctatca gccccgcttt gccggggctg atggtatttg
     9241 cgaatgaaaa tgttaattgt ttagtataat tatctgcagt ctttatttta ttatatttaa
     9301 aattgttccc catgagatat ttattttact tagttcgagc atttgccttt ctgatgatta
     9361 tggtatttat gctgtttttg gctcttttca agaagatgtt cagtattttt cctgtgtcga
     9421 ataaaaagca tttgctgaat aaaagaatag atggcaacct taatttgtta atgtattata
     9481 aaaaccgcaa ttcctggttg gtaaattaat ggtttttatt gttgaagtta acacataact
     9541 attgacttct aaagttgaag taacaaaatt tgaacacaca acaagtttgt gtatttgtaa
     9601 aaagagatta agttccgata atttattttc gcataatcca ggcatctttg tagcctgaat
     9661 tttgtgcttg tagcaaagct tcctcagctt cacttttggt tgcaacagat gcaatagcaa
     9721 ctatctccaa tccattttta ttctttccta ttaatgaaga tgaaaaccct tttgacttaa
     9781 gttcagtatt taagtttaat gcattatcat aaactgaaaa gcatccgcca acaacatgat
     9841 attgtcttgt ataacttgaa acagcaggtt cagattcagg taatttcttt gtttctataa
     9901 ccggttgcgg cataggttct tcttcaacag tagcaatttc tttttctggt gtagagccag
     9961 ttatttgact tgcacttgaa tctgctattg gtttttcggt ttcgtatatg tttttctcag
    10021 ttacaggtga ttcagaataa atcaaatcct ctttggattt ttgctcttgc ttgttgttat
    10081 ttaaagcatg gtttgcttgt gagtcaattt ctgttgtaac tgggaaaatg gtagcaagac
    10141 ccttagttat ttttgagttg atagccggat taaaatatgc aaatgcaatg aatgcagcca
    10201 ctcctgtgta tgcagcaatt tttaaaatca acttagcgtg atttgttcgc tttggtgccg
    10261 gttgaacagc cgcatctctg ttgctgaaaa tttcaaaagg tctgtctcgt tgaatcgcag
    10321 gtgattgaac cgtacttaat ccgaacgagt ctgtcaaaaa gttttcatcg tcagccggaa
    10381 taaactgtag gtttttttca ggatcaaaaa ataatacccc tattttttca atttgaatac
    10441 gttggttggc ttcaagttgc gctttacata aagtcacaaa atcattaatc agtttgcagg
    10501 aatcgctata tgaaagcaat aatttctgac tgatataatt tgctaaaagc ccatcgtttg
    10561 ttgttaaatc gctgttgaat gcaacaattt ttgatggagg cgaaaacaaa tgttgaaccg
    10621 gatgaatagt tgcattttta tatcgggcaa caaagcctcc gaattgtgga actatcacac
    10681 aatcatgctg atgcagaagt tcaaaaatat aattgccgat tttcatttat caggcaaata
    10741 tataaaaaac tttatgcctt agaccatttt tctgaaatct gtctctaaat tatatttctc
    10801 tgatgcaaac tttaaaaact tgtcaaacat tttgatattg tgacgagtaa gcatttttgg
    10861 atgtgagata aaatgcatgt attcgttagt gttcaaatac tttttatatt caccttgttt
    10921 gattattgac atcagttcta tagcaatgcg ctcccatgca gaatccagaa tatcatgacc
    10981 ttgttgctgc ggggtgacta aatttttatc aatttttact gctatttgcc cttcaccgcg
    11041 cgaaaaacta tggtcattag taatcttgta atataatttc agaaagagtt tctcaagtaa
    11101 ttttattgat tgtggaatat aaattgatga aatattaaat tcagtaaaac gaccattgtt
    11161 ttgttctgtt gtaatatcat tttcaaattg atagatagtt ttttgtggtg cttttgagaa
    11221 gtcgaaatat tgtgcattgc taaacagata aaaaccacct aacacactaa agtcaaattt
    11281 gatattatgc ttgatgaaat agggtaagaa agcattgaaa ggttgaatgc accatccgcc
    11341 tgcacggtag ctatccacta aataagaagg attaaccttt gtaacacact gttgaagtag
    11401 ttctattgat tgatcaaaaa tttcagaacg ttcagcttca cttatatgat aaaaacggta
    11461 tttgtcagtg tttattaatt gccattcatt ggtatctgaa agatagtttg catcaagcca
    11521 gtgtgggtga aggtgaggga atatttcatg accacgactt gcaatttccg taatgtgatc
    11581 catcacagtt tcaaaatcct gtttacatgc tgcaatttta aaatttttct ttaaagtcag
    11641 aagataggtt gtatcaacaa aaaaaatagc tctgcatcca tgctgctcta taacatccag
    11701 cacttgattg gttggattaa taaggcaatc tgcaaccgac ccgcttctgt tgcctaagaa
    11761 taattcataa tcaaaagtaa ttaatacctg cttggtgggt ttcatatttt ttcagacaag
    11821 aactacttta cttatgttta tctattgctt tcaaataaag cgcttctatt tcagcttcaa
    11881 tctttttttc ggcaaacctg cttcgacatt cttccgagag tttatgttgg tcgaatgtgt
    11941 tgtattcttc catcattttt atcattgcat ttgcaagatt atctattgat gccaaagagg
    12001 tgattccatt tttactatgt ataaaatcac ttggcccgcc cgagtctgtt gtgactattg
    12061 gcctgccaca tgctaaactt tctattagca cgacaccaaa tgtttcataa ttacttgaaa
    12121 gtacaaatga gtggcaatgg tctaattctc tttttacttc agcccgtgat agggcaccta
    12181 aaaaatctac tttatcggtt aatgctaact cgtttacata atcttttagt tcattataca
    12241 atggtccatc accacctata cgtagtcgaa tgttatttat tcctttcgct gtaatgtatt
    12301 taattgaatc aagtaacagt ttgtgattct ttcttggtaa tagaaatgag ttggtgaaga
    12361 aaacgaattc ttcctgttga ttaaattttt ttatgattaa gtctttaaaa aataaatcag
    12421 ctaccatatt gtgtacaacg gtaaaggtag tttcagggag atttaaaact ttttctatat
    12481 catgcttaaa gttttcgctt actataattg atgcatcagc attattaaat atttgtttag
    12541 cagtttctat atcttcctga tgtgtaatgc cgccagtaat ataagctgtt agatgttctg
    12601 taattataaa cggaagattg ttgtcctttg caatataata gccggccatt ccgccatgaa
    12661 aaacactgtg agcgtgtatc aaatccggtt tgccatattt cttaacataa ttataataga
    12721 ttttttcaac attttcgcta aacattttat aagccagctt tctcatctta ggtatgattg
    12781 cctgatgaat tacagaatat gttggaaggc cattgtcgtc ataaaaatca atgatttcat
    12841 ctttcttatt aaacattgag cttactgatg aaaattgcgg atatatgatt ccaactttgt
    12901 gtccggcaga catgagtgca cgcgcctgtt cttcgaaaaa tgttcccaga acaggttctg
    12961 tgatagactt ataccaagaa ggtataatta aaatgtgcat aaaaaattaa tttgtaattt
    13021 gatcaataat gtctttatac ttgttattcg gatgtttgat tttaataagc aaatcgtaca
    13081 tttttgcttt cagcggatta atatctgttt tccataaaaa acctttcttt agttcaccac
    13141 caaatccttt tttaaatcga aaaacccctt caagagcagg gtcgttattc ccaatacgta
    13201 cacctacaag atcatattta ttcactcccg attctttcat acgttcaatc atcttatagt
    13261 gtaaatattt cattgaacca tatagttttg tttcacctct gcttcctgca tgtgtgcata
    13321 atgctgcata gttagaatga atcataaaaa tgcctccgat aggattgtca ttatcatata
    13381 caacacctgc tgtaacatta tcgctgccaa gataattata ataagattta aaaaactgta
    13441 gctcttctga aggcatggaa attcttttca tagtatcagt atagcataaa taaaaatcgt
    13501 taagtacatc ttgtccgaat tttacaacag ctccattttt ttctgaatga tgaatagctt
    13561 tttgatattt gggatgaaac ttctgaaaga tttcctcttt tgtttgtgtc tgaaggtcta
    13621 tgatataggt gccaaactcg cagaagcgcg aattagcagg cacagaggca agtatgccat
    13681 acggatgtgg ctgaaccaat cgctcgcagg aattgttttg atttaaatat gaaattaatt
    13741 cgttaaaaaa atcaagttgt tcttgcggat taagttcaat attattttta atgggtgcat
    13801 gtaatatttg tgcaagtttg aaaaatcgtg aagagaaaaa tcgcaatggc ataaatgcca
    13861 ttaatttttc tgaatatgca ataaaaatat tttcttccat gtattcatga tacaattgag
    13921 taaacccggg aagaaactga aacggtgcgt cattattcat tgcggataac aatgactgat
    13981 cttttttctg taggtggttg agtaatagca tagtttattt caatagggta gaggcatgcc
    14041 tttcgctgga aagaaatttc atgcaagcct ctaatattcg tttggtagat tgtccatccc
    14101 atagtggagg aataactcct tttttgtatt tattatggac tattgcatca atgcgttccg
    14161 aaattttttc attactgaat ggtaccaatt cattagttcc gatatcaact gtacatggtc
    14221 tttctgtatt ggctcttaat gtaagacatg gtatttgtct gaatgtactt tcttcctgaa
    14281 tgcctccact gtctgtcaca atataagcac agtctgagat taatttctga aatgccaaat
    14341 aatctaaagg ctctattaat atcagcttct tgttttctgt aataaatgat ttcattccaa
    14401 actcttcaat tctgtgcaat gttcgtgggt gaattggaaa tacaagttga tgatttctag
    14461 ttataagttc aataattgaa aacaattgtt tcaggccatc tttatgatca actgttgccg
    14521 gacgatgcat agtcatcagg acgtactctt tgcttttaat atttaaagtc tcaagtatat
    14581 tactctgttg aattttttca ttaaaagcta caagtgtatc tatcatcgta tttccaacaa
    14641 agaacatttg tttttcttgt ttgccttctt gaattagatt tttataacca ctgtcttcgg
    14701 taataaaaaa gtaatcggtt atctcatcag ttaataaacg attgatttct tctggcattg
    14761 ttctgtcaaa acttcgcaga ccactttcaa catgcgcaat tttcaaattc attttattgg
    14821 ctgtcaatgc tgcagcaaac gttgaattca catcgcccac aaccatgagt aagtcaggtt
    14881 tgttttcctg aataactttc tctaatcgaa gcattatttc agccatttga gaattggggc
    14941 tgccggccgg tatgttaagc cagaaatcgg gtgttaaatc aaattgctca aagaaaatgt
    15001 cagccatctt gctatcatag tgctgaccag tgtgaactat ttttatatcg aaaggcatac
    15061 ctatgtcggc attcaccttt ttaaattgtg ttaccttaat aaaatttggt cttgtcccta
    15121 ctacaattaa aatctttttc attctcaata tctattcctt tttatacgta aaggtaagtt
    15181 taaagtttaa aattattgtt tcttagtttt atgtttcatc tgcaaaagca ttgcatcggc
    15241 aaaaaatttt gatgcccaat taggaacatc ataagcagaa taaccaccat ttacaggcca
    15301 tgagccggaa atgccgccat ttaccattgg cgaatgtgaa attaactgtt tgcttttaac
    15361 atattcaagc aaacgatcag cagtttctat gtattgagtt tcacctgttt gttcatataa
    15421 tatataaccg ataacactta actgacagtt gcctgtcagg cagcaaaacg cattgtcact
    15481 tgtccattct tcgtctaatt cgccagctag aaaaccgttt tgtttagtga gtgctacgat
    15541 gtgtttatat gagcgttcaa caatttcaat ccattcctgc ttttgaagat caatagctga
    15601 gcctagtaca ccccgcaatg cataaccgat agtgtgcgta gtgggtattt cattttcatc
    15661 aaaaccatta tcacaaaaat agtcatgctt attagtctgt tttatggcaa agttgatatt
    15721 atttattcct gctgaaatat attttgactc attcaaatgt tgacctagtt tgattaatgc
    15781 ccatgcagtt ctggtgttgg agctaagctg atgtaacgta aattcacgcc atgcaccatc
    15841 agagtcctgc acttttaaaa gccaatcagc actttgtata gcagcttgaa taacttcttt
    15901 tttctgactt tgctgatagt agtcagttag tgccataatt atttgaccat tattaaatac
    15961 tttgggaggt tggtttttaa aatctccata agatccggga aatgtaccat ctttacgttg
    16021 tgtagttaaa agccattgaa ttaactcttc aactatcggt ctttcattga atacttcatg
    16081 taataattct ggtttgttat ttttcagata gatgagtgtg tttatccaaa aagcagtggt
    16141 tcccggatat gcgggtagcc atttgttaaa catcattgag tacttggcag cacttcctcc
    16201 agttggtaaa agtgattgct tgaaccattc aagcgccata agtaatcttt gttcatcaga
    16261 aaagttttcg gctgttgatt gcgataacaa gttaaggtac cgcaacctaa tgtgtgaaat
    16321 cagccgttga atcatcgtta aaaattaaat tacttttgca attagttcac tatatgaata
    16381 tactcctgca atttcaccat ttttaagttc gtaaatgcgg tcgcagtttt ttagtgtagt
    16441 tacccggtga gcgatgataa agattgtttt attcgtatca gaaagtgagt caatggcctc
    16501 actaacctca cgttctgtct gagtgtctaa ggcgctggtt gcttcatcaa atacaagtat
    16561 ctgtgcattt ctataaagcg acctggcaat accaatacgt tgcttttgcc cacctgatag
    16621 ccgtgatcct ctttctccaa cttctgattc tactcctttg ggtaagtttc caacaaactc
    16681 atttaatgat gcttgtttaa ttgcagtatt aagtaaatcg gagttgattg tttcttctcc
    16741 aaaggcaacg ttttctttta tgtttgaatc aatcataaaa atatcttgct tcacatagcc
    16801 aattaatccg cgccagtatt caagattttc ttcttttaat ggtttgccat caactaaaat
    16861 ctgtcctgaa ttttcatcaa aaaaacgcag taagatattc atgagtgttg tttttcctga
    16921 gccggaacta ccaacaaaac caattttttc gcccttctta actttaaatg aaatactttt
    16981 caaaacaggc tcatgcgatt ttggaaatgt gaaactgata ttctgaaatt caattgtgtt
    17041 ttcaaataca attggaatat ttctgtccat ttctttttgc ttaacataaa gctcattata
    17101 ttcatttaaa ttttcaatgg caacctgatt tttagttacc tgcattactg cattcattat
    17161 cctgtttaaa gaaggcatca aacgatatgc tgctgcagca aatgcaccta ccatcataat
    17221 tacatcctgt tgcccgcctt caataaaaat tgcataaata aatatgacaa caattccaag
    17281 aatcgcaact aactcataac ctctgaaagg tatcatgttt atcacaattg aattcataga
    17341 taagtcgtga taagcctttt gatatttcaa aaaacgattc ctgtaatatt caattttgtt
    17401 tgccagttta atatctacaa agcccataat tgaattgttt attgcagcaa gagcctgagg
    17461 aaacatttta tccaaccctc tacctatctg ctcatttttt gtgttaacag acttatagag
    17521 tatgtatgat gcaggcccga taatcattaa tataaaaaga aagagtttaa ggttgtaaac
    17581 agcaatcagt gtcacaatgc agagtaagat tattgactct gaaaaaagca tcaaaatggg
    17641 taacataacc catgctgtat atgaggcagg gttttgctgt acgtgattaa taatacgtga
    17701 tgattttaca ctgttaaaat taaagtagct taaagagaaa tatttagaaa actgattttt
    17761 agaaatattg tttgcaacat cagccataaa ccgaccttca atatagttta cgaatattcc
    17821 aaaacctgtt ttgaaaaatt gaaataacag gattatcaca atagaaaata ccatgaatga
    17881 tttatctgtt tcaaaattca gataactata tgctgtactc aaataggtgt tggtatgaat
    17941 aatttcaggt tctgtagcca tttttactaa tgggagaaca gaagctaaac caaacacatc
    18001 taaaattgca gataaaaaaa tcagaaatgc aatccattta atccttcttt tttgcagtgg
    18061 ggataaatgg tcttgaagac ttttataggt ttgcctgaaa gcttgtgcag tagagtagtt
    18121 gtttttactg agcgacatct gttttgttcg tgttatttat aatatgctcc gccatagcca
    18181 acaggcatgt ttttaggatt tactccgttt aagataaagc tcagactctt gatttgattc
    18241 ttttccatca actgatgtgc aacttcaata aagtcttttt tgctatagcc ggctttcatc
    18301 acatacagat ttaggtctga atgtttcatc atcactaaag catcagacaa gagacctacc
    18361 ggtggcatat ctaaaatgat ataatcgaaa tgttctttca attcaataat taattgttcc
    18421 atcatcgaat ctaaaattaa ctcagaggca ttaggggatt ttggtccgct taagatgact
    18481 tttaaattct caacaggcgt atcttttatt gtatctttta aagaagcttt tccaacgata
    18541 tatgatgtta cacctacatc gttagtcatg ttccaagcaa cagcctgttt tggtttatgt
    18601 aaatccaaat caacaaccac gactttcttt tttgctctgg ctaacatggt agctgtattc
    18661 actgcacaaa aagtctttcc ttctccggaa atggtagagg tgaacagaat aacttttgaa
    18721 gatgttttag gagcaaaata ggataaattt gtcctgatga ctctgaatgc ttcagctgca
    18781 agcgactgtg gatgtgaatg tacaacaaga taatctttct tggcttctgc gcttttcccg
    18841 ataacaccaa tgatcggttg attagtaata tcggcaagtt cctctttagt aaaaatgtaa
    18901 ttaaggaaaa tacttttaag aaagactatg gctaacgcaa gtgcaagacc tacaccagct
    18961 cccataaata acattttgtt tcgaatggga cgtaacaatc ctgtacttga tgcaggttca
    19021 agaattgatt tgtccggcac aatggctgct gatgcaatca cattttgggc cctgctttcc
    19081 aacaagaata aatacatctt ttcattgatt tccttttttc ttgtaatatc actaagacct
    19141 ttaatagttg agggcattct gtttaatgat gcagcaaaat taccttcacg ttctgtcagt
    19201 aaattaattt gcattacaat atcctttcgg atattcatga ttattccaat gactttttct
    19261 tttgctttat taatttgtgc atcaacttcc ttaatctgcg gactatttgg tgtcatgctg
    19321 aaagacatat tcactttcct ttgcattaaa gcactcaatt cattaaaagc gttagaaagt
    19381 cctggattag cctgttcagt cattaatgct ggcgaaaggg tagtgaaatc actactgcca
    19441 cttgttaatt gattataaac ataatctaca gaattcagtc ttgctgttag tttagccttt
    19501 tcaccttgca catcaatttt ctcctttaaa aacgtacttt gctgattggc tacatcaata
    19561 gcaccgcttt gttgctgata ttcaaccaaa cttgtttcaa caccattaag ctgatcttcg
    19621 tagtctttta attgtgcttc aataaatttt aatgaattct cgtttatacc tcttgcaact
    19681 gaaatagagt aattgatata aagattcgta agcgtatcaa gaaagtcaac agctttttct
    19741 tctacctcgt cagcaatatt gatatttatt actgatgcat cttcgtcttt ttctaaacta
    19801 agcgcgtttt tgtatttctt aactaaataa ccatgattat taattacaaa cacatactga
    19861 aactcgctga attttggatt tttaagaata gactcctgct ttccgtttat aataattgaa
    19921 aatttatctg taactacagg gtcgccaaac ttgtgtactt tattgtatga atattcgttg
    19981 gtttttatgt caaggctgta agagtcctta tcaataattc ggaggttaaa aggaatacca
    20041 taaaaattgt catctaatat tttgccatca acactgaaag gtgtcccctt atacacctca
    20101 cctgttttta aacgtccttt tatgtaataa gaaatatcga ggtttagttt tttaattgct
    20161 tctgaaacca acttagttga tgataatatc tgtatttcat tagaaacctc ttctttattt
    20221 ggtcctaaag gaaggcttgt tgataaggca tctttgaaag cgtttttggt tggggtaatc
    20281 agcaccttag cctctgcacc ataaatgcgg gtagttttat aatatatgaa aatgcctccg
    20341 gcaacgccta tggccaaaaa caaaataaac cagtaccaat tctttacaaa caggtttagg
    20401 gcgtttttaa tgtccttagc atcaataatt gattggttct taattacttt ttgattattc
    20461 ataaaatcaa taatttaccg aacacactta tactcaaaac cggtagaaat ggttcgctaa
    20521 ttggtggttt aaaattgttt aatttgcaca aaattaaaaa attaaaacgg gttataattc
    20581 atgtttttta attttattaa ctaattgcag taactaagta gaaccacatt acgtttacat
    20641 atttttaatt ttaatcatca atgaaaagag cattaattac cggtgtcacc ggacaggatg
    20701 gcgcctacct gagcgagtta ctgctgcaaa agggctatga ggtacatggc attaaacgca
    20761 gaagttcact ttttaatacg gaaaggattg accacctcta tgaggatatg catcaaaaga
    20821 atgtgaaatt caagctgcat tatggcgatt taacagattc agccaatatc atcagaatta
    20881 ttcaggaagt gcaacccgat gaaatttata accttggtgc tatgagccat gtgcgtgtga
    20941 gttttgatat gcctgaatat actgccaatg ctgatggtat tggaactcta cgtattttag
    21001 aagctgttcg gatgttgaat atgactgaaa aatgtaaaat ctatcaggca tcaacaagtg
    21061 agttatatgg tttagttcaa gaagttcccc aacgcgaatc cacaccgttt taccctcgtt
    21121 caccctatgc agtagcaaaa ctttatgcat attggataat cgttaattat cgtgaagcgt
    21181 ataatatgtt tgccagcaat gggatattgt ttaaccatga atctccacta agaggagaaa
    21241 catttgtgac ccgtaaaatc acccgtgcag tatcgcgcat tgccttaggt cttcaggata
    21301 ccgtttatat gggaaatatt gatgccaaac gtgattgggg tcatgcaaaa gattatgtag
    21361 aaggtatgtg gttgatgttg caacaagata aagctgatga ctttgtgttg gcaacaggtg
    21421 taacaacaac agttcgtgat tttattgttc ttgcatttaa tgaagtagga atagaaatag
    21481 acttcagtgg taaaactgaa aatgaaattg cagtagtaaa aaaatgtaat aatccagcat
    21541 atcaggttcc tgttgggaat gttgttatga aaatagaccc gaaatattac agaccatcag
    21601 aagttgagct gctcattggc gaccctgcaa aagcaaaaaa agttttggga tgggaaccta
    21661 aacacaatct ggcatcaata gtaaaagaaa tgatgaattg tgaccttgag ctattcaagc
    21721 gcgaccaata tttgaaacaa ggcggacata aaattattaa ttatcacgaa gcataagata
    21781 atgcagaaaa acagtaagat ttttgtagca ggtcaccgtg gcatggtggg ctctgcaatc
    21841 gttcgtaaac ttcaatcgga aggatttaat aataacctgt tcagaacatc aaaggagctt
    21901 gacctgcgca accaacgtga cgttgctgat ttttttaatc aggaaaaacc tgagtatgtt
    21961 tttcttgccg ctgcaaaagt tggaggcata gttgcaaaca acacttatag agcagatttt
    22021 atttatgaga atctgattat tgaatgtaat gtcattaaaa gtgcatttga ttccggagta
    22081 aaaaagttgt tgtttctagg ctcatcttgt atttatccta aaatggcacc acaaccattg
    22141 aaagaagaat acttgctcac tggcccgtta gaacctacaa atgaacctta tgcattggcc
    22201 aaaatagcag ggcttaagat gtgcgaagct tttcgcgacc aatacggggt agattatata
    22261 tcggcaatgc cgacaaatct gtatgggcct aatgataatt acgacctaaa aaactcacat
    22321 gttttacctg cattggtacg aaagttttat gatgcaatgg aaaataatct gccatctgta
    22381 gaaatatggg gaacaggatc tccaagacgc gaatttttac atgttgatga tttggcggat
    22441 gcatgctttt ttctgatgca aaattatagt gaccgttcta cagtaaatat tggtacaggt
    22501 gaagacatca gcattaaaga attagcaatg cttattatgg aaaccttcgg ttataaaggc
    22561 caattgaatt ttgatacaac taaacctgat ggtacaccac gcaaactttt gtctgtcgat
    22621 aaattgcacc aattaggttg gaagcacagc atttcattgg aacaaggtgt tgcaagtgta
    22681 tgcaatgagg ttaaaagcat ggaatctttc ttggagcgtt aaaaaaaata ccctgtactg
    22741 agcgaaatct gcaggtgact gttaaaacgc gatagtaata atcataaaaa tagttatcac
    22801 atcaataaat atgatgtata ttcgtgaaat attaaaatat tttacgatga aaaaattcta
    22861 cttaaaattt attttatttc ttgcagtttc attgtgttct aagagcaatt tgattgcaca
    22921 aacacagaca gttgttattg gtacatctag ccttatatca gcatttagtc ctattgtcaa
    22981 atcttatgaa tgtgttgcct acgaatcaat atatctttca actgagatag gctattcagg
    23041 cgctatttct acttttggtt tcaatcgtgt tgatggtact gacaccttac cttttgatag
    23101 tgtctcaatt tttatgaaac agacagcttt gacttcacta acagccggag ttattgatac
    23161 aacaggatat acaagagtat atgatggtag tttcccaaat agtgcaggtg ccggatggcg
    23221 ggaagtctct cttaacctgc catttgctta tagtaattct tccaatctta gtgtgcttgt
    23281 gtttaagcac actcaggcag caacagcagg cactcccgtt accccaaggt ggtattatac
    23341 atctgtttct cctaacagag caagaagata ttatggaaca agtccaatgg catggggacc
    23401 aaacctgagt gtaaccaatg ttttaagtga tataagaatg accattggca ctgttggtat
    23461 tgttgatatt tcggcaggtt tgtgttctgt atatccaaat ccaacagatg gattggtaaa
    23521 tattgttgtt cataatgaaa aggtagcatc aatgttagta tctgatatat ctggaaaaat
    23581 tgttgctgat gttacaacgt tgaaacaatt gactgtggac ttaagaaacc tcagtaatgg
    23641 aatttatacc atacaatttt ttgacaatac aaaacaattg atggctgcta aaaaaatagc
    23701 tataaaaaag taagtgtttt tagatctgaa agaagcttga gtttcagata aattatttca
    23761 ctataatctt ctgaatattt attgatgaat ttgtttcaaa tttcacaaaa tatacacccg
    23821 gtttaaaatt gctgacatca aaaaaagcga agttttgacc atttgatttt gatgtatttt
    23881 gtgattcata gaccaacttt cctgtaacat cgttaatttt aatttgaaac attgttccaa
    23941 tatgactgga gtaggggaga caagtaattg ctgatgccgg atttggaaaa attttactaa
    24001 ttgtaattgg attatctaaa gcattgattt ttgttgtttg taatatatca aaataaaaat
    24061 atcctaacgg tgcaggcata ggtcgagtct gatgttttga tgtgtttgaa taggcttcaa
    24121 tataatagta aactcttgtt cctccggatt gggcaggtat gtatgtttca aagtaatcac
    24181 tcatccctgt taaagctgtc atggggacag cattgtaagg ctgaagcgta tctgttctgt
    24241 aatataaagt tgcagattga attccactac gatgcttaat aaatgcaact acagaataag
    24301 gatttattgt atcataagta tctgcaagtt gttgatgcga aatcagcagt gggtcattag
    24361 tgcctaaatc tttggtgata caatgcaatg ctcctaaggc accaatactt tgaagagagt
    24421 taatcccaat aacattatat ccgggtaatg catctctgta aatacgtaaa gcagtgctgt
    24481 cttgcgaaac attataggta ggaactataa tagtgttatt gatgaacgat gaattggtgt
    24541 atgtaaaata gtcgcctgtt gtatttggat atgctccatt atcagcaggc atcggaatgc
    24601 gaattacctt ataaggtgtg ccaaatacag aaaggaaatt attaacaaca taaagcaaat
    24661 tagcttcaat ctgtggtcca tcggcaacac cctgcggata ttgtcccatt aaaatagttt
    24721 cttcgtttaa aagcttcatg tgcatatcaa tatgatgaat gccatcgtac ggtaaagtcg
    24781 gcattttaat gtatctttca atgcccataa attgtttcat aattgtatct atctgacttg
    24841 ttgttaagga ggggttttcg ttcacaataa gattgcttga aaatcctgtt ccaaatccat
    24901 cacacataaa attgccaccc gtgtgaacca aactgtatgg cgacatagtc gtttgatata
    24961 atggtgtatt taatagccct gcaattgcca ccggaacatt atcatcatca gggcgcggac
    25021 gattatatat ccagtcaata gttactaaag tatcaacgtc atttgtgtat gcactccatg
    25081 gaccgtaatc tcgcgaccac actgaattga aaggtgtatt cacgaaactc acatttatcg
    25141 tatcaacatt tccactgttt aaatagttga ttacactttg ctgattattg gttacaatat
    25201 atacacgaca ttgtgtttta gcagcacgaa caatttcgcg caaaatagca ggataacttg
    25261 tccaaccaat caatatacct tgtaattctt cccactctgc catagtacga acccgtgatg
    25321 atggtggggc agcaacttga ttatttttta aattattaga aaattgctgc agataggatg
    25381 gcatctgcaa cacttctttt tgagtcatat agtttggaag attttcctga gcagaaacag
    25441 tactgcatag aaaaagaatt gccataaata atttaaaagt attttgcatg ttgaggctat
    25501 aatgaaacat caaatatagt cactttcaat aaattactat cactgaataa tatttgccaa
    25561 ataattgctt tatttgcttt gtaaaacaga aagtatgaaa tggatgggta ggcgcgaaag
    25621 cgacaatgtt gaagatcgta gaagattttc aggaggtaaa attgccttag gcggtgggtt
    25681 ggtcggcata attgttattg taatcaactt attaatgggt ggagatccga atcagctgtt
    25741 acaacaaatg cagcagcagg caacaacaca aacaacatca caagaaactt ctgctgccga
    25801 tgatgaaatg gcaagtttta taaaagtagc attggcagat aacgaagata tctggaagaa
    25861 aatttttcag gaaaatggga ttgaatatcg tgagccaaaa ttggtgcttt tcagagatgc
    25921 aacacagtca ggttgtggaa atgcaagttc tgctgttgga cctttttatt gcccagccga
    25981 tgaaaaagta tatattgatt tgtctttttt tgatgagttg aagagtaggt ttggtgcaca
    26041 gggtggcgat tttgctatcg cttatgtttt agcccatgaa gtaggtcatc atgttcagca
    26101 tttattaggt acatctgaca aagttcacga aatgcaaaac cagttatcag aaacagaagc
    26161 aaataaacta tcagttgcac ttgaacttca ggctgatttt tatgcaggag tttgggcaaa
    26221 tcagaatcag aagataaaca atgtgcttga agacggtgat attgaagaag cattgagtgc
    26281 agcaagtgca gtgggtgatg ataaaattca gaaaaaagca cagggttatg tcgtacctga
    26341 tgcatttaca catggcactt cggaacaaag aatgttttgg tttaaaaaag gttatcagac
    26401 cgggaaatta caagagggta atacctttaa ggagcttaat atttaattta atcactctaa
    26461 aatgaaaatt tttaaaaccc gttgaaacta attgttgttt atcgggtttt tatgtttatg
    26521 tataaaattc aaaatcaaat ttaaattatc atgcagaaat tcacaacatc gttaatggca
    26581 ctaatcattg ccacaacaac aacttcaatg gcgcaattaa aaacgccaca accaagtcca
    26641 actcaaagtc tgacacaagc atttggtcta ggtgaaatta aaatcgaata cagcagacca
    26701 tcagtaaagg gaagaacaat ttttggcgat cttgttcctt ttggtaaaat atggcgtacg
    26761 ggagctaatc aatctaccaa agtaactttt ggtgctgata ccaaggtgaa tggtaatgat
    26821 attgcggcag gcacttatgc actctacaca ataccaaaca cagcagattg ggaaataata
    26881 ttttataaag acttaactct cggaggcaat gttggcgact acaagaaaga agatgaaatg
    26941 atgcgtttta aggtgaagcc gatggcgact gccgataaga tggaaacttt cactatcaat
    27001 gtaaataaca ttgcaccaac ttcatgtagt attgatttat tatgggacaa aacaaaggtg
    27061 agtatcccgg taactaccaa tattgacgag aatgtcatga agcaaattga caaggcaatg
    27121 ggtgacaaca aaccctattt tcaggcagca aattattatt acgaaaatga taaagattta
    27181 aataaggcgc gcgaatggat aaataaagct gtggaaaaca atccaaaggc atactgggtt
    27241 ttacacacaa aggctaaaat tgaatacaaa ttgaaaaatt acccagaagc aacaaaggca
    27301 gcagagcaaa gtctggcttt agcaaaagaa agcagtgata acagctacat taaaatgaat
    27361 gaaaaattga ttgcagatgt taagaaaatg ggcaaataaa aagttattaa caatctgatc
    27421 ctcaaaacgg tattctccta catttgtgga gggtttattg tttttaggtt agaggggaac
    27481 aacataatag ttgtttccct tttttatttg tccttatgta aaatataaac tccaatctga
    27541 ttgtgttttt aattgtgaag taatggttta ttaacatcat tattcaataa aaataaaata
    27601 tgattgttga atcatcgttt tgattattaa ttttggaaaa aaattataca atgagaaagc
    27661 tagttaaacg tttgtctgca cttagtgcaa tgttgattat ctcaactgta gcattttcgc
    27721 agtcaactac tacacaggaa acagaacaaa ccacaacacc tccaacagaa actacagaaa
    27781 gtacggaaga ctctactgga gccgttgagg ctttacccga ttcaactgca gttgctccgg
    27841 aaggtgatcc tgctgccggt gcagccaacg aaaaacctgc cacaagacac aaattgtatt
    27901 caggcaagca acgtggcaac aatgtaattt atgatcctaa aaaataaagg gttgttttat
    27961 acttcatttc atcgtaattt tactttcata aaatataaat caatgagaaa attattaatg
    28021 agttcagcta tttgctctgt gttattatta agcaccatgt tgtcttttgg tcaacaggca
    28081 gaaaaaaaat caacagaatc aggcttcttt gttcgcaatg gccttgtcta ttacattaaa
    28141 ggcaacacag taaattttct ggaaagtgat caaacacttg ataacggtgc aacaataaat
    28201 cagcgtggaa tgataaaata tctcgatgga aagacagcac aacttcagca gggagatatt
    28261 tttacattca acaacgtatt agttccaaag gccgttgaag aacatcttga gttaaaagac
    28321 ggtaagcttc aaattgtaac taatggtatt tttactcctg tagagaaatc catgaaattg
    28381 gaaaccggtg tcacaattga tgcagaagga catacaacat caggaataac ccttatgcca
    28441 aaacaaaaaa tgaacatgat gggggaatta ataaaatagt attggtttaa aaaatatgac
    28501 taagccgggt aattccggct ttttttatgc ttaattttac ctgtcatgtt taagtttatt
    28561 gatcaaacag gatttgaaat aaaattggaa gcaccacctc agagaattgt ttccttagtt
    28621 ccatcacaaa cagaattgct ggcagattta ggattacaag atagcatagt tggtattact
    28681 aaatattgca tttatccacc cgaacttttt ttgtctgtac cccgaatagg aggaacaaaa
    28741 tctgtgaatc atgaattggt cagccaactg aaacctgatc ttattattgc aaataaagag
    28801 gaaaacaatc gcgattctat tgagcaactg agagagcaat ttcctgtttg gttaagtgat
    28861 gtgagtaatc tgcacgaagc attggatatg atttacaaca ttggtgtaat gactaatagt
    28921 caagaacgtg cttctgaaat aaataatcag atttatgata aatttaattc actaacttta
    28981 aagaataaaa aacatgttgc taagagagca ttgtatttta tctggcgaaa tccttatatg
    29041 tgtgccggtg gcgatacatt tataaatgac atgatgaata aagccggttt tgataatctg
    29101 atgtattatc ataaacgata tcctgaactg actgagaaaa gaatatcaga gttaaatccc
    29161 gaagttatac ttctttcatc agaaccatat ccgtttaaag caaagcatat acaggaattc
    29221 aaaagcattt gtcctaaagc tgaaatcttg ttggttgacg gagaattttt tgcctggtat
    29281 ggcagccgta tgattaaggc tgcagattac ttttatcagc tatgcgacag caattccaat
    29341 gtgtcagaat aatacctttg caaaatgatg cagaaatgga taaaattgct atctataatt
    29401 agtttgttaa atgtaaattt tttatttgca caaaaagtcg gtctggtatt aagtggagga
    29461 ggggcaagag gatttgccca tattggtgta ataaaagcgc ttgaagaaaa tggaattcct
    29521 atagattata ttacaggaac ttcagccgga gcacttgttg gaggcatgta tgtttcgggc
    29581 accagtccac aacaaatgca aaatatagtt ctaagtgaag attttaaaaa ttgggcaaca
    29641 ggttcgttga gcgatgaact catctactat tttaaggaac gtgaaagaga tgcatcgtgg
    29701 gcaactgtta aacttagcta tgattcgact ttaagaatga attttaatgg atcattagtc
    29761 aatagtggcc aaatagactt tgctctgctt gaaaatcatt cagcagctag tgccggtgcc
    29821 gattacgatt ttaataatct gtttgttcct tttcgttgtg ttgcctcaga tattaaaaat
    29881 aaacgcccgg tgatttttaa ttgtggcgat ctgagtcagg caataagagc ctcaattgcc
    29941 tatccactgt atatctcacc tatgacagcc gataatcagc tctactatga tggtggcata
    30001 tataataact ttccctcaga tgttatgctc aatgagtttg atcctgatat tatgattggc
    30061 gtttcagtgg gtggtggtcc tgatattccg aaagaagata atatgttttc actgctcaga
    30121 acaatgataa ttcaatcgca acatgttgtc ttgccgggag ctgatgatat attaattcaa
    30181 cctgatgtta ccaatttcgg tgtgtttgat ttttataatt ttcaatcgtt gatcgatagt
    30241 ggttataagg caacaatggc tcgcatggat gatatcaaac aagctattgc cagaagaatt
    30301 tcacccgaag agttgagccg gaaaagagat gactttatta agaaattaca ccctttagtt
    30361 gttggcgaca ttattgttac aggtgtaagt cctgaaaaag caaaatatat taagtctatt
    30421 ctgaatcctc gtggaagcaa tatgttactt cgacaactca aaccccgtta ttttagactt
    30481 cttgcagatg aaaatatcag atatgctttt ccaaaattgg tttatgatcc aaaaacagag
    30541 gtgtacaatt tagaagtcta tattaaacct gaaacagatt tacgggttga tataggagga
    30601 aatttttctt ccaggccaat tagcgaaggc tttattggat tcagatataa tgttcttggt
    30661 ccatatagtt taggcattca tgccaacacc tatttcggaa agctttataa ttccggacag
    30721 ttaaaattaa gactagattt accaggcaga cctgattttt ttatcgaacc gtttgccaca
    30781 atcaatagga tggactatta taaaagcagt agttttttta aagatgatat taagcctgct
    30841 tatttgttgg taacagatca atcctttggc ttaaatattg gtatgcctgc acgaaataaa
    30901 ggagcattaa ttgttggtgg aaattatttt aatattaagg acaggtatta tcagacaaga
    30961 aatttctctg tcaatgatac agcagacttt accagatttg aaggagtaag ttgtgttgtt
    31021 ggttttgaaa ggaatacttt aaatcggaag atgtatgcat caaagggttc ttacctatct
    31081 attcgcacac gttttgtttc cggcaatgaa ataaccaatc ccggatctaa acaagacaat
    31141 gcaccaacta tcagtgaatg gcaccaatgg tggcaggtta aaatgattta cgataattat
    31201 ttcttcagag caggaaagta taagctcgga ttttatgctg aaacaaattt ctcaggtcaa
    31261 ccattcttta ataattatac agctactatt ttacaaatgc cggggtttta tccgacccaa
    31321 gaaagtagaa cattgtttct cgataatttt catgcaccaa actatttagg tattggagtg
    31381 cgtaatgtgt ttagtttacg gtcaaatatt gatttccgtt tagagggtta tggatttcaa
    31441 ccttttcagg aagttgttaa gaatgaaaat gagaaggctg ttttcggtga tgcattatct
    31501 gcacgatatt ttattgcatc attttctgga attttccaca gtccggtagg acccgtagca
    31561 atgacactta attattacga cagaaaatca aagtcattta ctgttctttt tcatatcggc
    31621 tatattttat ttaataaaca tgctttggat tagccaatta gtctttttgg aaagatgcta
    31681 tgagtaaaac agtcattgta gctccattaa actgggggct tggtcatgca acaagaagta
    31741 aaactgttat tgataatctt ttaactgccg gttgtaaagt aattattgct tcagatggaa
    31801 atagtttaaa aatattgaag aggcaattcc cttttctgca cacagaagaa ttaccttcat
    31861 atagtcctgt ttacccttct gatggtaata tggcatgggc aatgctcaaa caagcaccca
    31921 aaatgctcat aaatattggc aatgaacaat cggttataaa gaagattgct acaaaataca
    31981 atgcagaaat tattatttct gataacaggt atgggtgttt tacaaatttt acaaagaata
    32041 tttttttatc acatcaactg atgattcaat cacctgaatc aatgttgttt ttgcaaccat
    32101 caattaacag gttacatgct aaatttataa atcgttttca cgaatgttgg attccggatg
    32161 ttgtgcaagg atttaagttg gccggaactt tatcagaaaa taaatatata aaaattccaa
    32221 aattttattg cggattactt tcgtcatatc tatcgtccaa tatgtcaaat gataataaat
    32281 ttgatatagt tgcaattcta tcggggccgg agcctcagcg tacaatattt gaaggtagaa
    32341 ttattgaaca gttggaaaag atccggtatc gttcgttatt aatacgcggg aaaatttctg
    32401 acccaaatga aatacctgat agaggagtaa tgcagtttaa gaattatgta tcagcagaag
    32461 gaatttctaa ttacattagc ccgaatacat ttgtgatttc aagatctggt tattctacaa
    32521 ttatggatat tgcacaatta ggtgtaagcg cattattggt gccaacaccg ggtcagacag
    32581 aacaggaata tcttgcatca tattttgatg agaacaagat tttcatgatg caaactcaaa
    32641 ataatattga tatacaaaaa gcattcgaga ggaagtcgga ttacttagct ataaaaaccg
    32701 agaagaatat tttattacaa caaaatttaa accggatatt actaacctag cttaaagcaa
    32761 gttcatattg cattcctgtt atggagttaa gttcactaat gcaaaacaat gcatcttcaa
    32821 tttgaatctc atcttttttg ataactgctt cataataccc ttctgtagac agccataagc
    32881 cttctggcaa atcaaccagt tcatgagcat ataatatcat taccaattca ccctcttcat
    32941 catatagttc atttattgta gcaggaaatt tttctgaagc tttaagttga ggattattca
    33001 tgcgtgaatt tagaatattc tttttatcaa tctgagtcta tgtgtgtatc gtttggtttc
    33061 ctgattgtaa ataccaacct gatcaaatgc atctattcga acttttcctg atgcatgaat
    33121 aatagtgtca cgtgaagtta atataccaac atgaataata ttgccttcct cattatcaaa
    33181 aaaagctaaa tcgcctggca tggcttcatc aacaaggttc actatatttc cttgttcaga
    33241 ttgtaaagaa gcatcacgca aaagtgagta tcctgataat ttataaacca actgcgtaaa
    33301 tccggaacaa tctatgccaa atggtgacct tcctccccat aaatagggtg cattgagata
    33361 catatatgca ttttcaatta ataatgctga tgtaactgct ttttgaggaa actttgcatt
    33421 accatcaaac cgatatgttc ttgtattaat tctgcaatgc tgattttcaa aaaagggtaa
    33481 agcacttcct aatattactg tctgtatttt attatcgtgt aataagagtt gcaccaagtc
    33541 aaagcagaca aatgctccgt ttttttgcag aaattgaaaa tcgttatagc taatttcagt
    33601 gtgttggttt aaagcaataa atccttcata accatcatgt gaagttcgaa tcttaaacca
    33661 ctctttttgt gattcaacaa cttcataatg ctctccaaaa agtatttgtg aaaccaactc
    33721 acttctgtgt gaaggctcct tcctcatagg aacaagactc atttcacaaa tgccgaatga
    33781 catattctgc taagtattga atttaagcca atgcatcaac tacatcttgc cttgttacaa
    33841 tgtagtttct gtcagcttta aaatctttta caataactgc ctgattgttt ttactaagca
    33901 agtcacttaa tgtatcaatt gaagtattga tatcaataaa aggcatagca tcttgcataa
    33961 tatcactcac cggtttattt tttagttcag gactttttaa gagtgatgaa taaatatgat
    34021 tctctgtaat atttccaact attcttcctt cacgcgttac cggaagttga gaaaagttat
    34081 ttgttttgat aattttcaat gcgtcaccta tcggtaatgt tgtctcaaca gtagttaagt
    34141 taacatcttt ttttgttccg ataatatctg atgctgtcat gcctttctta tcgaaataac
    34201 cttttgaacg catccagtca tcattaaata tttttgcaat gtacctcgat ccatgatcat
    34261 gaaaaataat tacaaccaca tctgttggtt tcaattgatc tttcatctga agcagtcctg
    34321 caacagcaga gccggcactg ttacctaccc aaataccttc tttgcgaacc aaatcacgtg
    34381 tcatcaatgc agcatcttta tcggtaactt tttcaaaatg atctattact gtaaagtcgt
    34441 aattctgtgg aataaaatct tctcctatac cttctgttac ataagggtaa atttcattct
    34501 tatctaaaat acctgtttca tgatattttt taagtgcaga accataagtg tcaattgccc
    34561 atactttgat tttcggattt ttttctttca aataacgacc agtaccggta attgttccgc
    34621 ctgttccggc accaactact aagtgggtga ttttaccttc tgtctgctcc caaatctccg
    34681 gaccggtttg ttcataatgt gcctgtcgat tacttaaatt gtcgtactga tttggataaa
    34741 aagcatttgg aatttctttg cttagtctgg cagcaacaga ataataggag cgagggtctt
    34801 ctggttcaac attcgttgga caaacaatta cttctgcacc aaatgcgcgc aatgcatcta
    34861 ctttttcttt gctttgttta tcagtggtag taaaaataca tttgtatcct ttaacgattg
    34921 cagcaattgc taaccccatt cctgtgttac cgctagtgcc ttcaattatt gttcctccag
    34981 gctttaactt tccgctcttt tctgcatctt ctatcatctt aagtgccatt ctgtctttaa
    35041 tagaattgcc gggatttaca gtttcaattt tcgctaatac tgtagcttga acaccttgag
    35101 tcactgcatt caatttaacc aatggtgtgt tgccaatggt ttcaagtata ttgttgaaat
    35161 acattaaatg ttatttgaat tgattaaata attttatttc aagcttcttt tacaagtgca
    35221 ggcattctcc atttcgcaat tgcaaataca ccaaaaagta gcatgttgaa aaataaatca
    35281 cccataatag tgcctccgaa aaatggaact gcacttacgt agcaactcat taatccagca
    35341 atattaagtg gatacattaa ccctgtagtc catactccga agttggtcat gataaagaat
    35401 agtattgatc ccattaaaga tgctgcaact acattctgta cttttaattt atttctgagt
    35461 aaaaaaccta atggtgtaat taaaataaag cttaaataaa tccaaaacca tccttcataa
    35521 aatgcactgc ctggtttgat aagctgtaac aaaagatcgg aaattaataa agctgccaaa
    35581 ggaaggccaa aggctatttt cttatcttta agaaaagtgc caccaaaaag cgccatagct
    35641 ccaattggag tgaaattata aggatgtggt agcaaacgtg aaatggcaac tatcaaaatt
    35701 gatattgtta taaatttcgt cttgttgtct tttaaaatgt tattcataat tcaaaaatat
    35761 tttaatggca aagataggcg tatgaaagta aagattcaaa agtttattca atgcttttta
    35821 aacgaggttt aatggtgcta tacaggccat taggagtcta ttaattgtaa ttccagattg
    35881 tccaggaagc ttcagcttgc tcaattagca tgtcataccc attacatacc ttacaccttc
    35941 ttcttatacc ctcattcata aatgctgtaa tttcaggatt gtaatttagg tcaaaaagaa
    36001 tgtgcttgtc ggttaacttg gagtaattta ttggaggctt ttgggcaatt tgtgggaacc
    36061 tccccattgg tgtacaattt ataattagta aattttggct tatgatatca tctgattgaa
    36121 ggtaagaaat tgcactttca tttgcgtttc ttgaaacaaa cttaaatgca atactttttt
    36181 gtttcagaac aaacgctact gccttggccg accctcccgt gcctaatatc aatgttggag
    36241 gtttttgatc tccgataatt cgttcaagac ttattttaaa accaataatg tcagtattgt
    36301 atccaattaa tttctcatct tttatcagta ttgtattgac tgagccaaca gtttttccgg
    36361 tttcctctaa ttgatctaaa tagggtatta tacttgtttt gaatggagaa gtaacattta
    36421 accctgaaag ttgattgttg ctgattaatg cctttaaatt acttaagtct cctatttcgt
    36481 aattgttgta attacagctt atgtttagtt tcttgaattt atcagtaaaa aaaatttgtg
    36541 aaaaggaatg acttagtgga aaaccaatta aaccaaaatt tctcatttag aagaaaattg
    36601 ctgcttaata atccctatta atatgggtat taatgaaatt ccaataatag caataacaac
    36661 taatgaaaag ttatttttaa caatctctaa tctgccgaaa aaatacccgg cccaaacaca
    36721 aatgttaatc cataatacac ttgctaaaac actgaatcct ataaagcgat tataggtcat
    36781 actaccaact ccggcaacaa atggagcaaa agttcgaaca attggaacaa aacgtgcaaa
    36841 tattactgct ttgccaccat gtttttcata aaattcttgt gttttgttca gataatcttt
    36901 tttgagaaaa cgataatttt tttcaaaaac ttttggacct atataattac caataaaata
    36961 gttggtgtta tcgccaccaa aagcagcaaa aaagaaaatt atcatcagat aaaaaacatt
    37021 taaaggatta tcaggagggg cagctaatgc tcccgcagca aacaacaatg aatcgcccgg
    37081 caaaagtgga gttacaacca atcctgtctc agcaaatact ataagaaata gaataagata
    37141 aatccatgta tcataccgtt gtacaagttc gaataaatgt ttatcaatat gtaaaataaa
    37201 atcaattaaa aattttaagt gttccacagg tttaatttat atgacgttaa gtgttggatc
    37261 aatttcttgt gaataggctt ttataccacc ttttaagtta aaaatgtttg taaacccata
    37321 ttgtgtagta agtacttgat aaattgctgc actccttgac ccagacctgc aatgtataac
    37381 aacgggcttg tcttttgcaa tttcttcaat tctgtcaatg atttctccca taggaataag
    37441 cattccatta agattacaag catcatattc gtattcttca cgaatatcaa tcaactgaaa
    37501 gttttcacct tcatcttgcc attgttttaa ttcatgaact gttatttctt gcatgatttt
    37561 aattcgaaaa aattagagcg ccaaagatac gaattgactt tactttgata tcggtttgtt
    37621 gaacattctg ttcagattct gtttgctgaa ctgtattata aaagcaataa aaactaccaa
    37681 aaggcatccg gctaaattta accataaaaa tgccatcaca tcaaaatacc agaatgttat
    37741 tataattgct tcagtaatta aagctgcaat aaataccgag tttccatcaa gttgttttac
    37801 gaaaatggca gagatgaaaa tacccaatat agtgccgtag aataaagatc caagtatgtt
    37861 tacagcctca atcagatttc ctgtattgcc ggcaaagaat gcacagatta tacaaaagat
    37921 accccaaagt aaagttgcaa ttttagaata gtaaagtttc ttggtctcgc tatactcttt
    37981 ttttgatgac ctgaaataga aatctacaac agaagttgaa gtaagagaat taatacctgc
    38041 tgctaaagaa cccatggatg caagaaaaat tattgctata atcaatccga taagcccttt
    38101 aggaaaatga tgcatcacaa aatgcagaaa aacaaaattg gtatcattta tatcagcttc
    38161 cggattaatg gtcttgatta ttttaacagc ttcctgtttt acttcattgg cttcagccga
    38221 agtttgaagt atcttggttt tagtgaattc tgcatttgca agatcattac tgcgtaaata
    38281 ttttaaatag tcttttgata gattagagtt actttcgtta atattattga acctaaattc
    38341 taaactattg tagtagtcag ccatcggact ttccttagca attttttcta tgttctcatt
    38401 aaagaaaatt ggaggagctt gaaactgata gaatgaaaaa acaagaatac caataaataa
    38461 aatcacgaac tgcatcggaa ttttaaccat cccattgacg attaaaccta atttacttgc
    38521 tgatgaatcc ttgcccgtta aataacgacc aacttgcgat tggtctgtac caaaatacga
    38581 tagttgcagg aaaaagccac ctatgatgcc actccaaaca ttatatcggt tgttccaatc
    38641 aaatttaaaa tcaataatat tcattctgtt atgtatgcct gcaattttca gactttcgta
    38701 caaggtagca tcagttccta ttaaagacag tacaatgacc cctgctataa taatgctcga
    38761 aaaaataatt gtcatctgca atacctgaga atgcgataca gcaagagtcc cgccatagaa
    38821 agtgtaaatt attaccagaa cgccaattat cattgttgtg taaaaaattg gaatgttaag
    38881 tactgtagat agtacaattg aaggcgcata aatggaaatg ccggtagaaa ctcctctttg
    38941 caataaaaaa agtagagcgg ttaacttcct tgtcttatgg tcaaaacgcc tctctaaaaa
    39001 ttcatatgcc gtataaatgt taaattttct gaacgcagga ataaatgtgg cactaaggaa
    39061 aatcattgct aggggaagtc caaaataaaa ctgaacaaaa cgcatcccat cactgtatgc
    39121 ctgaccgggt gcactcaaaa atgttatagc actggcttgt gttgccatca cacttaaaca
    39181 tacattgtac caaggcaatt ccctgttggc aattaaataa ctatctaatg ttttcttatg
    39241 acgacttcta taaataccat agataacaat tgccagaaga gttatcgcta aaaccagcca
    39301 atctattata ctcatgaaaa agtcttagta attatatagt ataaagtaat ctgaataatc
    39361 aatagagcaa caacaattaa atagaggcgc atccatggaa tatctttctt cataactact
    39421 gataatttaa acttactaat tctgctactt ctttcccgac taaatatcct aatgccacac
    39481 ccattccacc taatcttaca gcacaataaa tgttaggctt tatttcttgg ataataggtg
    39541 atttactttc acccaaaccc attatgccac tccaacgctg ttcaatttta tattcaacac
    39601 ctgataaaat tatatcttct aatagtttct ctattgatgc ttgaatttgt tctgtcacac
    39661 caaaatttgt tgttgtttca ccttgaaaat ttaaatttct gcccccacct ataagtactc
    39721 tttttcccac atttctgaag taataataac cgctttgata atgaaagcta ccgcataatt
    39781 ttaaatcttt aatttcattt gttatcagca cctgtgctct accgggagta atggatatat
    39841 ctgacaaaaa ttcttttgca aatccattgg tacaaataac aactttcccg gcttttattt
    39901 gaaacccttc tgaactcatc aaggttacac ctgagctttc agtatgaatc tgtttaattt
    39961 ctagtccatt taaaattttt actcctgatt gcctgacttt ttcagataaa acaaacatca
    40021 tcttaccggt atctatttga ccttcaaatt tgttctgaat taagtttttt actttgctta
    40081 aaccaaaatg tgcaatacgt tcatttacta ttttataggt attgtctttt gttatagatt
    40141 taagtcgtgt atttaagtac tctaattttg attcgcattc caagaatgat tggctatcgg
    40201 tctctgtaaa aatttcaaaa ccggaatttt gttcatattc aatattttca tcacctaata
    40261 gttctcttaa tccaagcaaa cctttgtatc tttgttcaac aagttgaaaa accttgtatt
    40321 cagaatcctt atctaaatca tttaaaattt ctgaagcact gcctatgcaa gcaaatccgg
    40381 catttcttgt actggcacca tagggcaaga aaccacgttc aattattaat atgtttaatc
    40441 ttggattttt cttcttaata taatatgctg catgcaatcc aacaattcca ctgccaagaa
    40501 caacgatatc aacattgtca aaccattgcg acttttccca aaagcttaat tgcatattta
    40561 taggcacgaa tatgataact tctaaattcc taaattaaat ccaattctga taatagggtt
    40621 tgtatatggt gattcagcag agtcattcag attgaacaga agtagaagat atagtccggc
    40681 attgttactg aaccgttgaa tataaccacc gccaacaaat ggtgatataa tattttttct
    40741 gtaataatca ttaatattta aatcataaag ttccatgttt aataattcga cttcagcatg
    40801 agcaaataag ttgtttgtaa ataaataacg accaaatatt cgacctccat aaatgcttgt
    40861 gctatatgta tagtaactat ctttatattt ataatatata taggttatac ctgcacctgc
    40921 tgatacggtt tcagtaaatt tatatcctgc taatggagaa atgtcaatat aggtttgagt
    40981 cccaaattgt aatccaaggt taccgccaaa atatattctg tccttaaatg gtattttgtc
    41041 tgacttaatt tttgatttat tttccctgat aggaggtatt gtgtcagttt gcgctgatag
    41101 tatttttgga aagcaaatca gtaaaaaata taatattgat ttttttatca tctttattct
    41161 caattttgca tgtaaaagta atttaattct caaacattgc acataatatg aatatagtag
    41221 tcactggcgc ttcatcagga ataggattag aattggtaaa atcatttact ttacaaaatc
    41281 ataatgtact tgcagtttca agaagtaagg agaagctgac cgaattaaag cgattttcag
    41341 aaagtattca tattttatca attgacatca acagattgaa tagtgaagtt attactgaga
    41401 cactaatcaa gtcaaaaatg aagcacattg atattttaat caacaatgca ggagtactcg
    41461 tgaaaaaacc ttttgtagaa ttaaccgaat ctgattggct tgaaacttac aatactaatg
    41521 tttttggtgt tgcaaatatg attcaatgtt gtatgccata catattgaaa tctcagatca
    41581 ggcatattgt caatatcagc agtatgggtg gagtaggagg gacactaaaa tttcctggac
    41641 tatctgcata tagttcatct aaaggtgcgg taacaatttt aacagagtgc ctggccgagg
    41701 agtttaaaag cgatgcaata aaagtaaatg ggttggcatt aggagctgtg caaacaccca
    41761 tgttggaaaa agcatttccc gaattaaaga catttcaaac tcccgaaaat attgttccat
    41821 ttatttcctg gtttgcttta gaggctcata aatggtgtaa tggaaaaatt atgcaggttt
    41881 cagattcaac gccttaaaat actttaacct ttcttaattc accaaagacc atattacttt
    41941 aaggtaaata cttctttcag gagtaggata acttgtggtt aaaaagtagt tccttcctga
    42001 taacccttta tttatatgat tacatcctat ttcaataact gcatttgaca tttttacctc
    42061 aaacgaaaaa tcaaaaaatg gatagttacc ggactttttg taaccatcct ttaagtaaaa
    42121 actaccagaa gctggttcat aagaataact ttgaaatgaa ctcaagtaat taagacttat
    42181 ctgtggactt actgcgattt tattattctt aaaatattga gtatagcaaa ctcggctacc
    42241 caaaattatt tttggaaatc ggtagatatc gttgtttgta attgatgaat agatgaaata
    42301 attagtaata tgaaattttt tcacctgaat atttgtataa agtgtcaatc tgttgatatt
    42361 aattgctgca ctaaattgtt ttggactagc tgaataatca aaatagatat gattcttaag
    42421 atttgttaaa gaatacttaa taactaaaat agaatctatt atagaagaag ttaaatcaaa
    42481 tgatttgtaa ttgactaaac cgaattgatt tttccattta tagtggtttg aataaaatct
    42541 gttttcaata tatgagggca tttgtttttt tgattcaatc ttgccatcta ttctccattt
    42601 tgatattggc aaataataaa acaatataga atggttggag tgattataag catcttgatt
    42661 tcttagaaaa gcaaactcac caatataaaa taatccatat ttgtttgtat tttgtatccc
    42721 acctccccaa aagagattgg aaacatagta tgttgaatca ataccatttt gagatacttt
    42781 gttaagcaaa ccgttatagc ctaaatgtcc attaatattg atttttaagg ctgaataatt
    42841 tttatttaat tcaagctttg atgaataagt gagtggttta taaacaaata aatcatctgt
    42901 tgaagttgta tcaatatagt ggttttgata aaagtcttct ttgttgtctg attcaaacca
    42961 gtattttaat tccgaatata caaatgaatt gagccatctc aagttccagt cttgcgtttt
    43021 gtttaatgaa tcaatatttt tttgtaaacc tgtattgaat aatctaatat tcaaatctgc
    43081 tccaaactca gacagttttc tggtagaact tgaagtagct agattagtag taaaaaattt
    43141 tgaattttgt tctttaaata tgaatagtga atcagaactc agacctccac tttcatttga
    43201 ttttatttta ttatttaaga catgaaatga ataattaact ctgtttaaag tgtcaaaatt
    43261 aaatgccccc acttcaacaa aaatcttttt ataggcagtg cgtaaataat aaccatctgt
    43321 cttaagcgta ttgaaattta cgaacccgac aagtgatggt gataattttt gattgtgttg
    43381 aacaaacaaa gaattgtact tctttgacgc agtttcaaat tccaaatgac ttaacgcctt
    43441 ctttgtgctt ttaaattcag atattttttc attagtcccc aatttttgaa tgaagcatgt
    43501 agtttcaata gggctttgat tgaattcaac aaaatcaatg actggagaat tatacttttt
    43561 taaagatttt ataaaattgt tttcaatggt tagtggagta gagccataat atttgttgtc
    43621 aaaactattt atttgaccaa acagattgtt gaaaaatagc agtgaaatga cacaaaagat
    43681 tagtgaaaaa gtcattttca tctgtttcaa actacaattt aaattttccc ggataaaata
    43741 aagaaataaa attgaatttc ttggtcttgt atggataaaa aaaaggctac caaattcttg
    43801 gtagccttaa tatttacggc aacgacatac tatcccacat gttactgcag taccatctgc
    43861 gctaaggggc ttaacttctc tgttcggtat gggaagaggt ggacaccctt gcaatagtca
    43921 ccataatttc ttaaagaaat taatgacaat acgtggcaaa aaataataaa ataaaattta
    43981 tgattaaagt ttcgggtaat tagtattgct cagctttgac attgctgtct ttacacttgc
    44041 aacctatcaa cgttatagtc tttaacgacc cttataaaga agtcttatct tgaggcaagt
    44101 ttcgcgctta gatgctttca gcgcttatct taaccgaacg tagctaccct gcggtgcagc
    44161 tggcgctaca acaggtacac tagaggttcg tccgactcgg tcctctcgta ctagagtcag
    44221 gccctctcaa acttctaacg cccacaacag atagggaccg aactgtctca cgacgttctg
    44281 aacccagctc gc
//
LOCUS       DAOQNP010000015        41974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_278_length_41974_cov_9.000048, whole genome shotgun sequence.
ACCESSION   DAOQNP010000015 DAOQNP010000000
VERSION     DAOQNP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 41974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41974
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_41974_cov_9.000048"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="PKY09_03645"
     CDS             <1..145
                     /locus_tag="PKY09_03645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809104.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNB11978.1"
                     /translation="RTPYRLHIRRPCFIYYQAYPEMIKGAYLSDAILTMSSMNVIAGE
                     LDA"
     gene            160..702
                     /gene="nuoE"
                     /locus_tag="PKY09_03650"
     CDS             160..702
                     /gene="nuoE"
                     /locus_tag="PKY09_03650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNB11979.1"
                     /translation="MSANKNIQFSDDALALVQKIINRYPEGKQKSALLPILHIAQAEF
                     DGWLSPEVMDYVASLLKIQSIEVYEVATFYSMFNMKPVGKCLIEVCRTGPCWLMGAED
                     VVRYIEKKLGIKSGETTADGMFTLKTVECLASCGTAPMMQIGETYHENLTLEKVDDIL
                     NDHKNQGNRRTHWEKDKIKP"
     gene            713..2050
                     /gene="nuoF"
                     /locus_tag="PKY09_03655"
     CDS             713..2050
                     /gene="nuoF"
                     /locus_tag="PKY09_03655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNB11980.1"
                     /translation="MSRKLLLDNLDKPGIQSLTGYSDNGGYEALRKALQMKPEEIVSE
                     VQKSGLRGRGGAGFPTGMKWSFIAKPEGVPRYLVCNADESEPGTFKDRYLMEHKPHIL
                     IEGMIISSFALGANTSYIYIRGEMMYVFHILQKAIDEAYAAGYLGKNILGSGFNLDLH
                     VHCGAGAYICGEETALIESLEGKRGNPRIKPPFPAVKGLWGCPTVVNNVETIAAVVPI
                     IKITGEEYAKIGIGKSTGTKLISASGHINKPGVYEIELGVPVEEFIYSDAYCGGIKNG
                     KQLKAVVAGGSSVPVLPAHLILKTAAGEPRLMTYESLADGGFATGTMLGSGGFIVMDE
                     DTSVVKNLWTFAHFYHHESCGQCSPCREGTGWMYRILNKILNGKGEMKDIDLLWNIQG
                     NIEGKTICPLGDAAAWPVASSIRHFRSEFEAYINNHGMKDVKHYYRLNNLQEA"
     gene            2050..3024
                     /locus_tag="PKY09_03660"
     CDS             2050..3024
                     /locus_tag="PKY09_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNB11981.1"
                     /translation="MPKVTIDGHTIEVPEGTTILEAARKIGGDIVPPTMCYYSKLKTS
                     GGYCRTCLVKVSKGSEKDPRPMPKPVASCRQTVMDGMEVQNITSPEILDARAGVVEFL
                     LINHPLDCPVCDQAGECNLQDLSYEHGHEVSRLDFRKRTFPMIDIGDKIKLHMNRCIM
                     CYRCVKVAEQLCPERVHGVLNRGWDSEISTYIEQAVDNEWSGNMIDVCPVGALTDKTF
                     RFKSRVWFTKPVDAHRNCPTCSGKVVLWYQGEEVLRVTGRKDQWGEVDEFICNECRFD
                     KKKTSDWVIEGPRHIDRHSVISQNHYEELKKLKQDLSRQKKLGEKAHN"
     gene            3034..4074
                     /gene="nuoH"
                     /locus_tag="PKY09_03665"
     CDS             3034..4074
                     /gene="nuoH"
                     /locus_tag="PKY09_03665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNB11982.1"
                     /translation="MEVSVLIFKTVFVIAVFLVSLLIAMYSTYAERKVAAFLQDRVGP
                     NRAGPFGILQPLADGVKMFMKEEIIPSHADKALFILGPSLMMLTACMTGVVIPWGDTL
                     IINGKEYFMHITDVNIGVLYIFGVVSIGVYGIMIGGWASNNKYSLLGSVRASSQMISY
                     ELAMGMSIIALIMVTGTLSVREIVVQQQGFHWNVFYQPLGFLIFLICAFAECNRTPFD
                     LPECETELVGGYHTEYSSMKLGFYLFAEYINMFISSAVISALYFGGFNMPFISELGLS
                     HNVLTLLGVLFYFIKIFFFIFFFMWVRWTIPRFRYDQLMNLGWKMLIPLSILNVLLTG
                     VFLLWKNGLFHF"
     gene            4091..4660
                     /locus_tag="PKY09_03670"
     CDS             4091..4660
                     /locus_tag="PKY09_03670"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB11983.1"
                     /translation="MTKELLIVGGPNGSGKTTFAKSFLDEYNYEFLNADEIANELSED
                     GYQAGNISAGKEYFNRIEKLKTKNKNIILESTLSGMFLIKLIKEFKKNEYNVKIIFIV
                     LNSPEQCIERIKHRVVTGGHFVPDDDVRRRFIRGKNNFWNIYKSIVEDWKLFNNTETG
                     FELIAAGEKNKFDIVNQSLFEFFIKDIKK"
     gene            4657..4875
                     /locus_tag="PKY09_03675"
     CDS             4657..4875
                     /locus_tag="PKY09_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11984.1"
                     /translation="MKSIKFKKYKNNNLFKEALEFAAIGNRGVQLAMIDSQKRNIPTV
                     FGIMDMVFYKLPDGTITTKSPFKKRKKI"
     gene            4901..5446
                     /locus_tag="PKY09_03680"
     CDS             4901..5446
                     /locus_tag="PKY09_03680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNB11985.1"
                     /translation="MMLTNRSKVVVNKEMTFAEKIYIPAIVAGLMITIKHFFKKKATI
                     NFPEQKKPISEVWRGLHVLKRDEQGRENCTACGLCAVACPAEAITMTAAERKKGEEHL
                     YREEKYAAVYEINMLRCIFCGLCEEACPKEAIFLTNRMTPSDTLRDNFIYGKDKLVEG
                     IAPEERVDISYRARAHRKEVE"
     gene            5446..5766
                     /locus_tag="PKY09_03685"
     CDS             5446..5766
                     /locus_tag="PKY09_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11986.1"
                     /translation="MGSLKKLFSGVIVTLSLALMSCDKDVCRDCYLVRSMLCTYTDTA
                     LAPYIIPGDTVKTEQQCDKEKDFKDDKFKEEEYEYDSPSMFYPNYTVHVVVTDIYHCP
                     EKTN"
     gene            5872..6327
                     /locus_tag="PKY09_03690"
     CDS             5872..6327
                     /locus_tag="PKY09_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNB11987.1"
                     /translation="MSALFTVFSKKPVYSVLYLIFFFFTLFGHYILLNAQFLAAVHLI
                     VYAGAIMVLFLFVIMMLNLNKEIEPNKNIVSKFAAVISSGLLLIVIVSVLKHADVEVS
                     KLNQHTDIGLVESIGHVLYNEYMLPFEMVSILLLTALIGAVMLGKKEIK"
     gene            6328..6636
                     /gene="nuoK"
                     /locus_tag="PKY09_03695"
     CDS             6328..6636
                     /gene="nuoK"
                     /locus_tag="PKY09_03695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNB11988.1"
                     /translation="MEPININHYLLLSAILFCIGVAGVLFRRNAIIIFMCIELMLNSV
                     NLLLTAFSNQHNDASGQVFVIFIMAVAAAEVAVGLAILVMIYRNVKSTDIDVLNKLKW
                     "
     gene            6640..8541
                     /gene="nuoL"
                     /locus_tag="PKY09_03700"
     CDS             6640..8541
                     /gene="nuoL"
                     /locus_tag="PKY09_03700"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNB11989.1"
                     /translation="MDFLVSAIPLVPLIGFLLNGIINKKMPQSLVGIIASGSVLISFL
                     FSCYLFLQISNGTIKESYYSVFSWINAGSLNLGLSFLVDRLSVIMLLVVTGVGFLIHV
                     YSIGYMKHDEDYSRFMSYLNLFIFFMLLLVMGSNYVVMFAGWEGVGLCSYLLIGFWFK
                     NNDYNKAANKAFIMNRIGDLGFLLGLFLMYQTFSSLEFKDVFAAAGNYPSGDATLTAI
                     ALFLFIGAVGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIIRSNVIYTLA
                     PFAGEIVAVIGLVTALMAASIGLKQNDIKKVLAYSTVSQLGYMFVALGVGAYTTALFH
                     VVTHAFFKALLFLCAGSVIHAMSGEQDIRRMGGLKKYLPITHFTFLMGVLAISGVPLF
                     SGFFSKDEILAVTWTYSPVMFTVLAITSVLTATYMFRLYWLTFYGDFRGTHHQKEHLH
                     ESPSSMTIPLIILAVFSVIGGYIGMPEYINEKLGTHHVFTQFLNPIIYSPKEFILSHT
                     FEFTLLIGTIIGLAIIYVMTKNMYVRDKVVPEDDSQTKSFQKVLANKYYIDELYELII
                     RKPLEAFGELFYKIFDISIIDGIVNFIPRATNLTSSGLRLLQNGSVGFYLVSMVIGII
                     LIFVFKMLL"
     gene            8542..9999
                     /locus_tag="PKY09_03705"
     CDS             8542..9999
                     /locus_tag="PKY09_03705"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017256772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNB11990.1"
                     /translation="MNNLLLIILFLPLAGMALSLLPSREATGKTALVFSLISLLLSVC
                     LFIQFDPNGGMQQVLNYAWVPDLGINFKIGVDGISLMMILLTNVLSPLIILSSFGRRI
                     ENPKGLYALIQLMQFALIGVFISLDAFLFYIFWELALLPIYFICLLWGGKDRIRITLK
                     FFIYTITGSLLMLVGLIYLYLQTPGSHTFDIQAFYDLTLSSQQQSLVFWLIFVAFAIK
                     IPVFPLHSWQPDTYTDAPTQGTMLLSGIMLKMGLYGILRWLLPVVPAASADNAFIVML
                     LGIIGVVYASCIAIVQKDFKRMIAYASIAHVGLITAGLFTLKVEALEGAVFQMLSHGI
                     NAVGLFFIADMLMSRLKTHNMSEMGGIANNNSLFAVLFMIVLLGTIALPLTNGFVGEF
                     LLLTGIYQFKAWMALFGGLTIILGAVYMFRSYQAIMLGSRHLNEGEFQPMTLSEKTVL
                     GTIAFLVILLGVYPSFLMNAAAPSIKSLLMIINAG"
     gene            10025..11401
                     /locus_tag="PKY09_03710"
     CDS             10025..11401
                     /locus_tag="PKY09_03710"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNB11991.1"
                     /translation="MNTIVTLSIVGIAAMLSEVFKLKRIMYSLVIAGLIVALGFSIND
                     WTNFGSYFNQMVMVDSFSVVFSSLMIVTTLLWMLMSRNYFNSESSMSEHFALIVFALT
                     GGVMMTTFSNMSILFLGLEILSFSLYIMAGSDKTNIKSNEAAMKYFIMGSFATGFLLF
                     GITLIYGATSSFNIQEIANYTQQNKDALPAFFKVGVLMILIGLSFKIAAAPFHFWTPD
                     VYDGAPTLVTAFMASVVKIAAFAMIYRLFVSCFITIYDFWTPVFAVLSAITMLAGNIT
                     AVYQTSLKRMLAYSSVAHAGYMLMALAAPGIASKNALLFYLAAYSLSALGAFNLLYSV
                     HKVTGNEGIKAVKGLFYKHPVSAIMLTICMLSMAGIPPVAGFFGKYYLFTAALKENLL
                     WLVLIAVLSSLIGIYYYFKVIIAAFQQPETENTGYCEKPSDLAILGICAVLSLLLGIA
                     PQLISGLL"
     gene            complement(11658..13226)
                     /locus_tag="PKY09_03715"
     CDS             complement(11658..13226)
                     /locus_tag="PKY09_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11992.1"
                     /translation="MNKKLSTLLFIFGTFISYGQFVFQKTYGGNNSDYGLTVINSGDG
                     GFIIGGSQVSSASGGGFLAKLNSAGDTLWTKTLLSNSVQAIAKTDDHGFIIGGMSGTG
                     GASNQGFGIFKTDSSGTILWNTNIIRSGYINSSTSVLQAYDGNYIAAGTSQEFVFNYY
                     SQFYVTKVDTSGNLLWAKVYQASGLNYCTQIIQTHDSCFVLIGSSDYPSNSTNIIKIN
                     SAGNVIWAKNLSAPGNGFIGHSIQETNDQCFVITGWNFQNGLFVFDVSLIKIDSIGNL
                     LWRKSFGGNFNDYGFRIKQTPDNGFLIAGYTESFNMNNWDSYLIKTDSLGFLLWSKTY
                     GGSGSEQCYDMDLSSDSGCIMIGFTSSFGLGYNDCYIIKTDFIGNSGGCFEINPQTLN
                     DTISIMSNNLIIQVINEGYQFPVYAFTSSFGDVSVACMGNVGVYDFFTETKNIIVYPN
                     PSTGIFTISSSDNFANAEIEIYNVLGDRIYSDNINNSNLKKICIDKMVDGIYFVKVSD
                     REKMYIGKIIIETN"
     gene            complement(13477..>13884)
                     /locus_tag="PKY09_03720"
     CDS             complement(13477..>13884)
                     /locus_tag="PKY09_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB11993.1"
                     /translation="PVIDYLVSDDYYYWFHDTIKQPLLTYGITTIDGTAQTPVLRYQP
                     ISGTTGISQIAAPSEITITPNPSSGKFLLSNISSTNALLEVVNTLGQKVNFQQTNKEQ
                     SVEVDLSNLPKGVYFIKVTDGRTSSTHKVIVEF"
     assembly_gap    13885..13984
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13985..14367)
                     /locus_tag="PKY09_03725"
     CDS             complement(<13985..14367)
                     /locus_tag="PKY09_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11994.1"
                     /translation="MNKTFTLIATLFCAAAIKTSAQLVTISATHQLPAIGDTIRYVDA
                     NTFGFNQNGTGPVTNTLWDNALLMNAGTNYLFFYDDPATITGNGVDSFPGATLARGES
                     GATGYFYYQNTANDINRIGWYSSAS"
     gene            14619..15845
                     /locus_tag="PKY09_03730"
     CDS             14619..15845
                     /locus_tag="PKY09_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNB11995.1"
                     /translation="MTEIMSPAGSFESLQSAIQGGADAVYFGIEQLNMRARAANNFTL
                     SDLAEIKKICTAKNVKTYLTLNTVMYDHDIMLMKKIIDAAKKNDIDAVIASDHAALMH
                     ARKIGIPVHISTQANVSNIESVRFFADYADTIVLARELTLNQVADVVKQIKRQKIKGP
                     SGELIKIEVFAHGALCMAVSGKCYLSLHSDNASANRGACIQNCRREYIVQDKENEIEL
                     EIDNEYIMSSKDLCTIDFIDKLIETGISILKIEGRGRSADYVYTTTKCYKEAVESYHN
                     GSYNATKAEHWKKELATVFNRGFWGGYFLGKTMGEWSNTGGSQATTKKIYVGKGVKYF
                     PKSKVGEFKLESGTLSVDNDIMITGSTTGIILTKVQELRMDDLPVTTVKKGDVFSMPL
                     AEKIKSSDKLYKIVND"
     gene            15838..16065
                     /locus_tag="PKY09_03735"
     CDS             15838..16065
                     /locus_tag="PKY09_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNB11996.1"
                     /translation="MIEIVQYRSKCIGCNACVEASATRWRMSRKDGKCTLVGGKNNKG
                     IYITVVNDDEYEQALQAAKNCPVNIIKVKKK"
     gene            16066..>16255
                     /locus_tag="PKY09_03740"
     CDS             16066..>16255
                     /locus_tag="PKY09_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11997.1"
                     /translation="MDAQIENFIGSQKNMTLCTSINNTPECANCFYTFMPDGDYLIFK
                     SKKTTGHVSHALVNNKVAG"
     assembly_gap    16256..16355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16356..16643
                     /locus_tag="PKY09_03745"
     CDS             <16356..16643
                     /locus_tag="PKY09_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB11998.1"
                     /translation="KKTTGHVSHALVNNKVAGTILPDISKIGSIKGIQFSGTFLTPQD
                     DMLEQAKKIYYSKFPLALGISGDLWLIKLTYIKMTDNTLGFGKKLLWENKN"
     gene            complement(16657..19521)
                     /gene="gcvP"
                     /locus_tag="PKY09_03750"
     CDS             complement(16657..19521)
                     /gene="gcvP"
                     /locus_tag="PKY09_03750"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNB11999.1"
                     /translation="MYYSFADRHIGPDDKQVKHMLATIGVKSVNDLIAKIIPELIRLQ
                     KPLNLPEAMSEFEYLNELKAVASENQLFKNFIGMGYYNTIVPGVIKRNIFENPGWYTA
                     YTPYQAEIAQGRMEALLNFQTMVCDLTGMQIANASLLDEATAAAEAMVMFYYTRSKEA
                     EKKNAVKFFVSENCFPQTIDVLRTRSKPLGIELVVGDEQLFSPDDSFFGALLQYPDAI
                     GNVLDYKNIVNMCHRHDVRVCVAADILSLVLLTPPGEWGADCVVGSTQRFGVPMGYGG
                     PHAAYFATHDVYKRNLPGRIIGMSVDAEGNPALRMALQTREQHIRRDKATSNICTAQV
                     LLAVMASMYAVYHGAEGLKAIATTVHKRTTALAESLKKAGFNVVNNSWFDTLTVEVED
                     AESVRDMAEANLINFRYKGNSVGVSIDETTSDEDLKDILRVFAASIGKKSIAAVSLNG
                     NAIPATLLRSSEFLTNAVFNTHHSETEMLRYIKRLENKDLALNHSMISLGSCTMKLNA
                     TSEMEPLSWPEFANMHPFAPVEQAQGYAHIITELENYLKEITGFAAVSLQPNSGAQGE
                     YAGLVVIQKYHNDRGQNHRNVVLIPQSAHGTNPASAVLAGLKVVVVKCDDDGNIDLSD
                     LRLKAEQHQENLSCLMVTYPSTHGVYEENIKQITAAVHANGGQVYMDGANMNAQVGLT
                     CPALIGADVCHLNLHKTFAIPHGGGGPGMGPIGVAEHLAPFLPSHTVVPTGGRKGVNA
                     VAAAPWGSALILLISYGYIKMLGKSGVQRATEIAMLNANYIKARLEKHYPVLYKGKNG
                     FCAHEMILDCRAFKAVANIDVTDIAKRLMDYGFHAPTVAFPVAGTLMVEPTESEPLEE
                     IDRFCDAMIAIRHEIMEVINGEYSKEDNMLVNAPHTALEATADEWKHSYSRQKAVFPA
                     PWVAGNKFWPSVKRVDNAYGDRNLVCTCPDVLSYETAEAI"
     gene            19876..20538
                     /locus_tag="PKY09_03755"
     CDS             19876..20538
                     /locus_tag="PKY09_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12000.1"
                     /translation="MNQITDMTLRHFITSLIFLFSVTTLSAQDTLLLLNGKTKPVKLF
                     NLNDPDWVRYVKLQGSSRVKKVDIYKVFSIKKADGTEQVLYKPDSTSDDPDIPWVRDF
                     IAGQQYGILHKRERFNKHDNQWHRRLNFTEVGGIAAGGAGSLLTFYGIPVPAIYAIAV
                     GRHSPALPNAPDISPEMKNSEGFISGYQKQRRNQRIRQGFISGMIGFAVGIVTFTLIE
                     RN"
     gene            20539..21348
                     /locus_tag="PKY09_03760"
     CDS             20539..21348
                     /locus_tag="PKY09_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNB12001.1"
                     /translation="MFRKIAHKAVSYNILIALAASAASHLTILRINEQNNVVLIATIF
                     FATLAIYNFPRLPLQMPLTSRTWVCLFSILATAILIFNYNYIAINLFLTAILACLIYM
                     MPFNYKGKRIKGIRNIFLLKNIFLALSWALITVLLPMAELGHESPIIKEVFLLRFIYV
                     FGISLLFDIRDTAKDKTRNHLTLPVVLGTTVTRYIVIASAALFILIVSVLYRHEHFSA
                     PMATAFIISTLLLVIAAITAPPFSNFKFYQVGVDSLLIVQWLLVVVAVKFY"
     gene            21454..22926
                     /locus_tag="PKY09_03765"
     CDS             21454..22926
                     /locus_tag="PKY09_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB12002.1"
                     /translation="MSKGIHVKKPLDSLLAEAEDSEKGLKRTLTANSLIALGIGAIIG
                     AGLFVRTAAAAANNAGPAVTIAFVIAGLGCAFAGLCYAEFASMIPIAGSAYTYSYTTM
                     GEFIAWIIGWDLVLEYALGAATVSIAWSEYLNKLLNYLGMHIPYEWSHSPFEHSLDGV
                     YGIMNVPALLILVLLSLLLIRGTQESAFVNNVIVITKVAIVVLFIVFGWQFINPANHT
                     PYIPAASTFTDHMGVPHHYGGILGILGAAGVVFFAFIGFDAVSTAAQEAKNPGRDMPK
                     GILGSLVICTILYILFAHVLTGVASVDDFRTQGREASVTYAIQTYMHGYDWLAKLVTV
                     AILAGFSSVILVMLLGQSRVFYSMSRDGLLPKVFSELHPVYRTPYKSNLILFVFVGLF
                     AAFVPGDIVGDMTSIGTLFAFMLVAAGVIIMRKTNPDAKRTFRTPLVPLVPILGIIVC
                     GAMIFGLGWPNWSRLLMWLALGFVIYFGYSAKHSKLRNPK"
     gene            22947..24401
                     /locus_tag="PKY09_03770"
     CDS             22947..24401
                     /locus_tag="PKY09_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB12003.1"
                     /translation="MHNDKPHLHRSLGLWDATMLVAGSMIGSGIFIVSADMSRHTGSA
                     GWLLFLWLLSGVITLIAALCYGELAGMMPQAGGQFVYIRRAWGNCLAFLYGWTVFMVI
                     QTGVIAAVAVAFAKFTAVFFPYFNTGNILFQAGGISISAAQMLAIFLIAVLTWFNSYG
                     IEGGKTIQNIFTSAKLLALFILIVAGLWVGLKTNTLSANFDNAWSVNSMIKTDSSWIE
                     QHISGFAIILMLGTAIIGSLFSSDAWNNVTFIAGEIKDPSRNIPRSLMLGTAMVTLLY
                     CLANIAYLALLPLKGSIDAATVAGQGIQFAGGDTDRVGTAAASMIFGDSSVYIMAALI
                     MISTFGCNNGIILSGARVYYAMALENLFFKQAKKLNSKGVPAIALWVQAIWASLLCLS
                     GTYGDLLDYTVFASLLFYVITISGIFILRKREPEAERPYKVFGYPFLPALYIILALSI
                     CSILLYTKTAVAGKGLLIVILGLPIYYLFKRRNS"
     gene            24465..26327
                     /locus_tag="PKY09_03775"
     CDS             24465..26327
                     /locus_tag="PKY09_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12004.1"
                     /translation="MRKISTLFAAMCITATVSAQTQRLVLFEEFTGETCPPCAQINPT
                     LNEMLNDEADKIVSIKYQNNIPSAGPRFWPYAQTDIVARQTYYSNSYSPQGIMDGNQF
                     NDNAGALDPVIINPRYATPSPFEVRVAHTFSAANDTIYASVIIKAAQNYNGSNLVAQI
                     AVTERDIFGYTSPNGESHYEGVLRKMLPSASGTPLQQSWNAGDSVVLNYSWYITPAAL
                     SSPVYYQLSVIAFVQENSNKEVLQAGFSRAQVPMDPKAKAITGMQDVTCTGNITPTFT
                     IANNGITDISTLDIQYKVDNNPAQTYSWMGLLPSFTNTTISLPPIPVSGNGSHSITLT
                     TTNPNGGVTDNNMLNNSKTAYFGVPVAAVAGNVLEDFALSTFPPANWIRVDQNGNNTG
                     WLRSTAGNGSAKIDFYSSFTGHIDNLYVYPVDMSGASVATMTFDVSHRQYSAAYIDAI
                     NVQVSTDCGLTWNTEWAKAGAALATVTGYQTGAFTPTTAAQWSAETVNLNQYAGNNNV
                     LIRFNAVSGYGNNAYVDNVNVSLSTGINEVANTVFELYPNPTTGIVTIRTKTQNSDYK
                     IHFSDISGKEVFVNTLIEGNIIKADFNTCSNGTYFAEIQTPNGTERSKFVLIKD"
     gene            26493..27467
                     /locus_tag="PKY09_03780"
     CDS             26493..27467
                     /locus_tag="PKY09_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNB12005.1"
                     /translation="MSKGPISQFMQHHYRHFNAAAMMDASKAYVTHLSEGGKMMVTLA
                     GAMSTAELGISLAEMIRQDKVHIISCTGANLEEDIMNLVAHNSYKRVPNYRDLTPEQE
                     RELLDNHFNRVTDTCIPEEEAFRRLQKHIHQIWKEAENKGERYFPHEYMYKMLLSGVL
                     EQYYEIDPKNSWMLAAAQKNIPIVVPGWEDSTMGNIFASYVIKKEMKATTMKSGIEYM
                     VWLSEWYRQNSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIDTPKFIVESDASIVAPLMFAWILDW"
     gene            27477..28019
                     /locus_tag="PKY09_03785"
     CDS             27477..28019
                     /locus_tag="PKY09_03785"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB12006.1"
                     /translation="MNTAVKYTLAGIAAVILGFISLGIIHPEIEYESRVQINAPIAKA
                     FDIFTDTAMMKHWMPGFVSFKPLEGEIHTKGSKQLLVLNLNNEKIEITETLEDYSYPK
                     LYQFHAQNTLLNSTNTIIFEGDSLQCNMTIKTKASGNNFMLRSMFVFTEPIFKQQQDS
                     TYTLLKRTIESHLDNHKINQ"
     gene            28066..29733
                     /locus_tag="PKY09_03790"
     CDS             28066..29733
                     /locus_tag="PKY09_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNB12007.1"
                     /translation="MNSRIINFLLLLLISTTVNAQSDAELKSNLKQHISILASDKFEG
                     REPGTKGERLSYEYITQQFKNIGLQPLGSNDYLQSFEFSAGTKNGAKNKLNIAGKSLK
                     SDTEYYVLPYSALQSTASGKSIKIGSGLLSKELGRNDYEGFTDLKGKIAVMDYATPEA
                     DNPHSKWAEASDLRKRIEIAVSKGISGLIIYNSNDSIKNPEKNSEIKITPFVIPIVFV
                     KSIDAARLKNDQQITIETDIEKIMHSGHNVIGYIDNHAGNTVLIGAHYDHLGYGGHES
                     LYRGTPAIHNGADDNASGTSTLIELARKLKSSSFKKYNYLFIAFSGEEKGLLGSNYFV
                     KHPTIDLKKVNYMLNMDMVGRLKPEEHVLLINGAGTSPSWDSLLNNIKVDSLRIKTTA
                     SGVGPSDHTSFYLEGIPVLHFFSGTHSDYHKPSDDEPLINYNGMVSIFHYMYDLIEQT
                     NKLPKLAFTKTNDSSNEDAPRFKVTLGVVPDYAFEGSGMRIDGVTEGRPAAIAGFLKG
                     DVVIQMGEEKITDMMSYMKALSKFKKGDKVKVKVQRGNEVVEKDVTF"
     gene            29746..30231
                     /locus_tag="PKY09_03795"
     CDS             29746..30231
                     /locus_tag="PKY09_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12008.1"
                     /translation="MTQRTIHFEEKQYLGLNHYSLLRSMVLALFCFVAYFVTENRENN
                     AELLFFGGVAFMVISLLSMFIKHLHTRVYDTCIELDGLWTMRKVKIDLSTIVAIQKIP
                     YSNYIMNTPVYNLHRNGSIYFYTSGRSAIKVTDKDGMNYIIGTQKQEEFLRILNSLIK
                     K"
     gene            complement(30288..30572)
                     /locus_tag="PKY09_03800"
     CDS             complement(30288..30572)
                     /locus_tag="PKY09_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12009.1"
                     /translation="MKKSVLTLAVVAAAYCANAQTEKKAQEQPVKSEQATTPQKEVKQ
                     DAPATIQAAPAKHEAEPATHPTDKAATKESPQKDAHKATEMEKPKDTPKQ"
     gene            complement(30631..31821)
                     /locus_tag="PKY09_03805"
     CDS             complement(30631..31821)
                     /locus_tag="PKY09_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB12010.1"
                     /translation="MLRTILSLLGISIGIFAIISVFTVVDAMEKNVRTSVESLGDKTI
                     YVQKWPWAFGGDYPWWKYWQRPLPSVKEMHQLAARSQSIEVFTFEAYLSGKTIKYLNN
                     SAENVTVALVSHDFDKVKSFELSAGRYLTEIESNSGRAVGIIGSNVANGLFGNASTAL
                     GKVIEVMGRKVEVIGVFKEEGKSLLETEMDNTVVLPLNFGRNFVNIRSDRIDPFIKAK
                     VKEGVPIQFANDELKSIMRSIRRLRPVEDDNFALNQSSLLSNNITNIFSSLNMAGAII
                     GGFSILVGGFGIANIMFVSVKERTSQIGIQKSLGAKNYFILMQFLFEAIVLCLIGGLI
                     GLSIIYFLAMFVSHAFDYPLSLTGHNIMLGLFISALIGLVSGFIPAYSASQLDPVEAI
                     RANG"
     gene            32016..33545
                     /gene="purH"
                     /locus_tag="PKY09_03810"
     CDS             32016..33545
                     /gene="purH"
                     /locus_tag="PKY09_03810"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNB12011.1"
                     /translation="MKIKSALISVYNKNGFEPLIKTLHQQQVTLFATGGTFDFIQSLN
                     IPVTAVEDVTDYPSIFGGRVKTLHPKIFGGILNRRNDAHDVMEVEKYNIPCIDLVVVD
                     LYPFEATVASGAEASDIIEKIDIGGVSLIRAAAKNFNDVAIIASANDVTEFLQTYNQG
                     NSEVDLSFRKKLAQKAFAITSHYDSAIFNYFYSKNEPSEPVLNLQFNNAVTLRYGENP
                     HQRGFYFGNLKETFEQLGGKELSYNNMLDCDAAINLIAEFSNEKGVTAAIIKHNNACG
                     IATREKATDAWQAALAGDPVSAFGGIIVFNQHIDESLAKEINAIFFEVIIAPSFAEES
                     IKVFSEKKNRIILQLKNQRVAKQSIKSALGGYLMQETDSEILNTEGWNNITLRKPTTA
                     ETEDMLFAVKVIRNLKSNAITLVKNKQLIGSGAGQTSRVDALKQAIAKAVNFGFNLKN
                     SVMASDAFFPFPDCVEIAKTAGIEAVVQPGGSIKDKDSIEYCNKNNMTMVLTGKRHFK
                     H"
     gene            33669..34691
                     /locus_tag="PKY09_03815"
     CDS             33669..34691
                     /locus_tag="PKY09_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNB12012.1"
                     /translation="MGLFDFLTQEIAIDLGTANTLIIHNDKVVVDEPSIVAIDRMTGK
                     VLAVGKQAMMMHGKTHENIKTIRPLKDGVIADFDAAENMIRGMIKMINPGRRFFQPSL
                     KMVICIPSGITEVEKRAVRDSAEHAGAKEVYLIHEPMAAAIGIGIDVEEPMGNMIIDI
                     GGGTSEIAVIALGGIVCDQSIRVAGDGFTMDIWDYMRRQHNILIGERSAEMIKIEVGS
                     ALPELDNPPHDYAVHGRDLMTGIPKEITVTYSEIAHALDKSISKIEEAILKALEMTPP
                     ELSADIYRTGIYLTGGGALLRGLDKRIGMKTKLPVHVAEDPLRAVVRGTGIALKNIDR
                     YKFLIP"
     gene            35068..35247
                     /locus_tag="PKY09_03820"
     CDS             35068..35247
                     /locus_tag="PKY09_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12013.1"
                     /translation="MEEQENKKKASGLQVVMDDVMLLLFLGVTIYAIFYIIWGIMEIA
                     TVPNIPESVKQSILK"
     gene            35269..36198
                     /locus_tag="PKY09_03825"
     CDS             35269..36198
                     /locus_tag="PKY09_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNB12014.1"
                     /translation="MTLIAPTEGWFKKKVSKDERMWIIISFVVCIFLFAWMVLWHVFG
                     RQNPSNTTYKTNPIEFAQLHDEYVKRNMVGMDNGIPIVKPEPGSDVILMAQMWRWSPV
                     LILKKDEWYNIHISSKDLVHGFSLQPSNMNFQVYPDYDYVLKFKPTVAGEAKVLCNEF
                     CGIGHHTMIGKILVIENDADLAKFGYNNLSAPPARTGKGTAEEDISGWSEEQLIKGGE
                     MVYNLKGCMACHKTDGTSEIGPAFNNIYGSKVNVKEDGNAKEMVVDDDYITMSIREPQ
                     KQIVVGFEKTPMAQMNLTDNEIKQVISYIKSLK"
     gene            36211..37878
                     /locus_tag="PKY09_03830"
     CDS             36211..37878
                     /locus_tag="PKY09_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB12015.1"
                     /translation="MFRICDITGFKVDLQSEKFIRLNAVMAVVSLLLAITAAILLVFT
                     RYQPVHLLDAEWYYRLTTFHGLNALIYWIVFFEIAGLYFGSTVVLNQKFCSPRLGWLT
                     FILMVGGMVLSNVMVLMGKADVMLTSYVPLKAHPLYYLGIILFAVGALMGVGLFFANI
                     VNAKRDGNVGETLPLFTYGLMTAAILAVITLASGAIIYIPTFLWSLGLIDSVDPAVYK
                     LIWWGLGHSSQQINVAAMVSIWYLVTFLALGGTSINERVSRTAFFLYILFISLASAHH
                     LLTDPATSSAWKVWNTSYAMYLAVLASMIHAFAVPSSIESAQRRWGFNKGLFEWLSKA
                     PWSNPAFAACVLACIGFGFIGGTTGVIFGMEQTNIIVHNTIAIPGHFKGTVVIGTTLT
                     FMGITYYLIPLIFRRKIVAFKLAQLQPWMFFFGVAILSVSMIALGQLGVPRRHWDSTF
                     SGGPFTHEFNPSVDFFWTLMMLGGTLAFLAALIWVLQVVVSVFFGPKVNGPEDMQLPL
                     SPLAPAAKEHKGYEAPGTLILTLLFLATFAVLFFVNWKWLAATWWVK"
     gene            37904..38680
                     /locus_tag="PKY09_03835"
     CDS             37904..38680
                     /locus_tag="PKY09_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB12016.1"
                     /translation="MIKIKIQLTGLVILLSLFFSLTSFDIPDEKKFIGREVKNITILD
                     SKGNNVQLFDNPTGKPVVLSPVYTRCPMACSLISNGLKTSIEKLGTLGKDFTVISFSF
                     DSTDTQEDLQGFEARWKMDGVNWKTVTSNAAGIDALLKSIDYQYEYDEKSKEYLHPNL
                     VVVITPSGRISRYIYGVEPKTIDLELAIMEAQVEKTSFNNFFKTLYIRCFTFNPATKS
                     YHLDWKFISQTSAGMMFIIIVGYFLIKSLYSKNTEDSAEL"
     gene            38677..40599
                     /locus_tag="PKY09_03840"
     CDS             38677..40599
                     /locus_tag="PKY09_03840"
                     /inference="COORDINATES: protein motif:HMM:NF025016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNB12017.1"
                     /translation="MINNKSSHWLFQRFAGIFSRIYSSKLNPLYHLGDIAILMFIVAC
                     ISGVYLFLFYNIDPAHAYESVEKISANPFNAVIRSIHRYSSDLLVIFIVLHFLQTLLT
                     GKFKRILSWVSGIISLMVVLIIGVTGFILVWDEKGKLVGYLTAKFFSAIPLFDPSIAG
                     SFISNNLDVIGGFFKVSVFGHIFFSIFLVIVLWLHVMRIAKPKLFSPRIIWIYSMIAL
                     VLVCLVFPVKSDPSAQERFIPYNTTFDWYYFFGYSFMKVVSVPMNWVIMLVSGTGLAL
                     VPLLFKKKNKPATIDLDKCDACNICSEDCPYGAIDMLIYKGERKAILDPDKCISCGIC
                     LASCKEHAITMPGIPEHNVLPLNLAQKSQLNVVSCSQFDDVKIPDGITHKKKSLPCLG
                     DLHMHEVEQMLNEESEGVLLLGCEDCYYRYGRNLAIARINRKRPPGLAKKLSRDRIQL
                     ITTNHCSEKAIRAFTDSLASAKPNDGKATIKVLDYFKTNHIAATLILCAFFLIMPFFS
                     NTKISFFDRDEKLLVLNFKYISSPTEFQGVNTNQQRQMQSLKPIVKRRSPIVVEVEGN
                     KGNVLLKKEFSPRGLRKDISIFVYSEIPTTDSLVNIKVTETAFPDKVVSLHNVALSTI
                     DGTIISFADGKLKQLK"
     gene            40712..41803
                     /locus_tag="PKY09_03845"
     CDS             40712..41803
                     /locus_tag="PKY09_03845"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNB12018.1"
                     /translation="MWRILTTIFFVSLLANSLSASKLQPGYNPKEYMELLSFQLSQFH
                     KQDSLYPDSMHYHNFKEVYKSPEMGLLNQWSLWIGADSVAVISIRGTIANSTSWLANF
                     YAGMIPAKGQLILAPGDTFNYHFADDSKAAVHTGWTLATAYLMRDMLPHIDSCYKKGI
                     TNFIVSGHSQGGAIAFLVTACLYDYQKNNKIASDLRFKTYCSAAPKPGNLYFAYEYEN
                     MTRNGWAFNVVNSADWVPETPITIQTTKDINKTNPLVNAKAGIRKQKFPANLFVMHIF
                     NRLQKTPYKAMRQYRKYLGGFAGKLVRKNLVTFQSPDYLNSSDYVRTGTTIVLLADKK
                     YYDLFPDSKTKIFVHHFYEPYLYLAAKRL"
BASE COUNT        13076 a         7690 c         7911 g        13097 t
ORIGIN      
        1 tcgcactcct tatcgtttac atatcagacg gccttgtttt atttattatc aggcctatcc
       61 tgaaatgatt aaaggagcat atctaagtga tgcaatatta accatgagtt caatgaatgt
      121 aatagccggt gaactggatg cttaaattta taatgtaaca tgagcgcaaa taaaaacata
      181 caattttcgg acgatgcttt agcattagtt cagaaaataa ttaacagata tcctgaagga
      241 aaacaaaaat ctgcactgct gcccatactg catattgcac aggcagaatt tgacggttgg
      301 ttaagtcctg aagtaatgga ttatgtagca tcattactga aaatacagtc tattgaagtt
      361 tatgaagtag ccacattcta ctccatgttc aacatgaaac ctgtaggtaa gtgtctgata
      421 gaagtttgcc gcacgggacc atgctggctg atgggtgcag aagatgttgt tcgctacata
      481 gaaaagaaat taggaattaa atcaggagaa acaactgccg atggtatgtt taccttaaag
      541 accgttgaat gtttggcaag ttgtggcaca gcgccaatga tgcaaatcgg tgaaacctat
      601 cacgaaaatc tgaccttaga aaaagttgat gatattttaa acgaccataa aaaccaaggc
      661 aacagaagaa cgcactggga aaaagataaa ataaaaccct gaaagataaa acatgtcacg
      721 taaattactt ttagataact tagacaagcc gggcattcag tcattaaccg gctacagcga
      781 caatggcgga tacgaagctt tgcgcaaagc attacaaatg aagcctgaag aaattgtaag
      841 tgaagtacaa aaatcgggat tacgtggacg gggtggtgca ggttttccaa ctggaatgaa
      901 atggagtttt attgctaagc ccgaaggtgt gccgcgctat ttagtttgta atgccgatga
      961 aagcgaaccc ggaactttta aagatcggta tttgatggaa cacaaacctc atattttaat
     1021 tgagggaatg attatttcaa gctttgcctt aggtgcaaac acatcctaca tctatatcag
     1081 aggtgaaatg atgtatgtat ttcacattct tcaaaaagca attgacgaag cgtatgcagc
     1141 aggatatctc ggaaaaaata ttttaggaag cggctttaat cttgatctgc atgtacactg
     1201 cggtgcaggt gcatatatct gtggtgaaga aacagcattg atagaatcgt tagaaggtaa
     1261 aagaggtaat ccaagaatta aacctccatt tccagcagtg aaaggtttgt ggggatgccc
     1321 gacagtagtg aataacgtag agaccattgc cgcagttgta ccaataataa aaataaccgg
     1381 tgaagagtat gcaaaaatcg gtattggaaa aagcacaggt acaaaattaa tatcagcatc
     1441 agggcacatc aataaacccg gtgtttatga aattgaattg ggagttcctg ttgaagaatt
     1501 tatttatagc gatgcctact gcggaggaat aaaaaacgga aaacaattaa aggccgttgt
     1561 ggccggaggt tcttctgttc cggttttacc tgcacacctc attttaaaaa ctgctgccgg
     1621 tgaacccaga ctcatgactt atgaaagtct ggccgatggt ggatttgcta caggtaccat
     1681 gcttggttct ggtggtttta ttgtgatgga tgaagatacc agcgtagtaa aaaatctgtg
     1741 gacgtttgca cacttctatc atcacgaaag ttgcggacaa tgttctccat gtcgtgaagg
     1801 aacaggatgg atgtacagga tattaaataa aattctcaat ggcaaaggtg aaatgaaaga
     1861 tattgatttg ttgtggaaca ttcaaggcaa cattgaagga aaaacaatct gtcctttagg
     1921 cgatgcagca gcatggccgg ttgcttcatc tatcagacat ttcagaagtg agtttgaagc
     1981 atatattaac aatcacggaa tgaaagacgt gaaacattat tacagattaa ataatttaca
     2041 agaagcataa tgccaaaagt tacgatagat ggtcacacca tcgaagttcc tgaaggaaca
     2101 accatactgg aagcagcccg aaaaattggc ggtgatattg tgccaccaac tatgtgctat
     2161 tattccaaat tgaaaacatc aggtggctat tgccgtactt gcttggttaa agtaagcaaa
     2221 ggctcggaaa aagatccgcg gccaatgcca aaaccggttg catcatgcag acaaaccgta
     2281 atggatggca tggaagtaca aaatattaca tcgcctgaaa ttttagacgc acgcgcagga
     2341 gttgttgagt ttttattaat taaccaccca cttgattgtc ctgtttgtga tcaggcagga
     2401 gaatgtaatc ttcaggatct tagctatgag catggacatg aagtgtcgag acttgatttc
     2461 agaaaaagaa ctttcccgat gattgatatt ggtgataaaa taaaactgca catgaacaga
     2521 tgtatcatgt gttatcgctg cgtgaaagtt gctgaacaac tatgccctga aagagtgcat
     2581 ggtgttttaa acagaggatg ggattctgaa atatcaacct atattgaaca agctgttgac
     2641 aatgaatgga gcggaaacat gattgatgtt tgccctgttg gtgcattgac ggataaaacc
     2701 tttcgtttta aaagccgtgt gtggtttaca aaacctgtag atgcacatcg caactgccca
     2761 acctgttcag gaaaagttgt attatggtat cagggagaag aagtgcttcg cgttacaggc
     2821 agaaaagatc aatggggaga agttgatgaa tttatttgta atgaatgtcg ttttgacaag
     2881 aaaaaaacaa gtgactgggt tattgaaggg ccacgacata ttgaccgcca ttctgtaatc
     2941 tcacaaaatc actacgaaga gttgaaaaaa ctgaagcagg atttatcacg tcagaaaaaa
     3001 ttaggagaaa aagcacataa ttaaatttaa gaaatggaag tttcggtatt aatatttaaa
     3061 acagtttttg taattgctgt ctttctggtt tcattgctta tagctatgta ttcgacctat
     3121 gcagaaagaa aagtggcagc atttttacaa gacagagttg gtcctaaccg tgcagggcca
     3181 tttggtattc ttcagccatt agccgatggt gtaaaaatgt ttatgaaaga agaaattatt
     3241 ccatcacatg ccgataaagc attgtttatt ttaggtccaa gcctgatgat gcttaccgct
     3301 tgtatgacag gtgtggtaat tccatggggc gatacattaa tcatcaatgg aaaagaatac
     3361 tttatgcata ttactgatgt aaacattggg gtgctttata ttttcggggt ggtttctatc
     3421 ggggtttacg gaataatgat tggtggatgg gcttcaaaca acaaatactc tctgcttggt
     3481 tccgttcgtg cttcttcgca aatgattagt tatgaacttg caatgggaat gtcaatcatt
     3541 gcattgataa tggttaccgg cactttgagt gttcgcgaaa ttgttgtcca gcaacagggc
     3601 tttcactgga atgtatttta tcagccttta ggttttttga tttttctgat ttgtgctttt
     3661 gccgaatgta acagaacacc atttgattta ccggagtgcg aaactgagtt ggttggtggt
     3721 tatcacactg aatactcttc gatgaaactt ggattctatt tgtttgccga gtacatcaat
     3781 atgtttattt catctgctgt aatttctgca ctttattttg gcggattcaa catgccattc
     3841 atcagtgaac ttggattgtc gcacaatgta ttgacattgt taggcgtgtt attttatttt
     3901 attaagatat tcttctttat ctttttcttt atgtgggttc ggtggacaat accccgcttc
     3961 cgatacgatc agttgatgaa ccttggatgg aaaatgctca taccattatc tatacttaat
     4021 gttctgttaa ctggcgtatt cttgttatgg aaaaacggat tgtttcattt ttaaaaaaac
     4081 aaaatgaaag gtgacaaaag aattattaat agtaggtgga ccaaacggaa gcggaaagac
     4141 aacctttgcg aaatcgtttc tggatgaata taattatgag tttttaaatg cagatgaaat
     4201 tgctaatgaa ttatcagaag atggttatca ggcaggaaat atttctgcag gaaaagaata
     4261 ctttaatcga attgaaaaat taaaaacaaa aaacaaaaac ataattctgg aatcaacttt
     4321 atcaggaatg tttctaatta agctgattaa agaatttaag aagaatgagt ataatgtaaa
     4381 aattattttt atcgttttaa attcaccgga acaatgtata gaaagaataa aacacagagt
     4441 agtaactgga gggcactttg ttcctgatga tgatgtgaga cgaagattta taaggggtaa
     4501 aaataatttc tggaatattt ataaaagtat agttgaagat tggaagttgt ttaataatac
     4561 ggaaacaggg tttgagctaa tagcagcagg agagaagaat aaatttgata ttgtaaatca
     4621 gtcattgttt gaatttttca taaaagatat taaaaaatga aaagcattaa gtttaaaaaa
     4681 tacaagaaca ataacctatt taaagaagca ttggagtttg ccgccattgg taaccgaggg
     4741 gtacaactgg caatgattga ttctcaaaaa agaaacattc caaccgtgtt cgggattatg
     4801 gatatggtct tttacaaact acctgacggt acaattacta ccaaatcacc attcaaaaag
     4861 agaaaaaaaa tataagtaca tttgtaatta ttctaatttt atcatgttaa ccaacaggag
     4921 taaagtagta gtcaataaag aaatgacctt tgcagagaag atatacattc ctgcaattgt
     4981 tgcaggtttg atgattacta taaaacattt ttttaagaaa aaagcaacca ttaattttcc
     5041 ggaacaaaaa aagccaatta gtgaagtttg gcgcggtctg catgttttga aacgcgatga
     5101 gcaaggtaga gaaaactgta ctgcctgcgg attgtgtgca gtagcctgcc cagccgaagc
     5161 catcactatg acagctgctg agcgcaaaaa aggagaagag catttatata gagaagaaaa
     5221 atacgctgct gtttatgaaa taaatatgct gcgttgtatt ttttgtggct tatgcgaaga
     5281 agcatgtcct aaagaagcaa tatttttaac taaccgtatg acaccatcgg acactttgcg
     5341 cgataacttt atttatggta aagacaaatt ggttgaaggt attgcaccgg aagaacgagt
     5401 agatataagc tacagagcaa gagcacatag aaaagaagta gaatgatggg ttctttgaaa
     5461 aaactttttt caggagttat tgtcacacta tcacttgcat taatgtcttg tgataaagat
     5521 gtatgccggg attgctattt ggttcgctca atgttgtgta cctataccga cactgctctg
     5581 gcgccataca ttattccggg tgataccgta aaaactgaac agcaatgcga taaggaaaaa
     5641 gatttcaaag acgataaatt caaggaagaa gaatacgaat atgattcgcc aagtatgttt
     5701 taccccaatt acactgtaca tgtagttgtg actgacatat atcattgccc ggaaaaaaca
     5761 aattaatcaa tagcaaataa tcaattaaaa caataatttc gcaccagttt ttcattctat
     5821 aatactaacg tgagccaata cgcattttac atactttcag cactttcggt aatgagtgcc
     5881 ttgtttaccg tattttccaa aaaaccggtt tacagtgtat tgtatctgat attcttcttc
     5941 tttacattat tcggccatta tattctgctt aatgcgcagt ttctggctgc agtacattta
     6001 atagtttatg ctggtgccat catggttttg tttttgtttg tgattatgat gttaaacctc
     6061 aataaagaaa ttgagcccaa taaaaatatt gtttcaaaat ttgcagccgt catttccagt
     6121 ggcctgttgc tgattgtaat tgttagtgta ctcaaacatg cagacgtgga agtaagcaaa
     6181 ctgaatcaac atacagatat tggtttagta gaatccattg gacatgtgtt atacaatgaa
     6241 tatatgctgc catttgaaat ggtttcaata ttactgctca cagcattaat tggtgcggtg
     6301 atgcttggaa aaaaagaaat taaataaatg gaacccatta atataaatca ctacctgctg
     6361 ctctctgcca tacttttttg tattggcgtg gccggagtat tgttcagacg taatgccata
     6421 attattttta tgtgtattga acttatgctt aactctgtga atctgctatt gacagctttc
     6481 agcaatcagc ataatgatgc atcaggtcag gtgtttgtga tttttattat ggccgtagcc
     6541 gctgccgagg ttgcagtagg attagctatt ctggttatga tttatcgtaa cgttaaatca
     6601 acagatatag atgttttaaa taaacttaaa tggtaggtaa tggatttttt ggtctcagca
     6661 attcctcttg ttccgctcat cggttttctg cttaacggca taatcaataa aaaaatgccg
     6721 caaagtcttg tgggcattat agcaagcggc tctgttctga tttctttttt attttcctgc
     6781 tatctctttt tacaaatcag caatggcact attaaggaat cgtactattc tgtattttcc
     6841 tggatcaatg caggaagtct gaatttaggt ctttcatttt tagtagatcg tttatcagta
     6901 atcatgcttt tggttgttac cggtgtagga tttttgattc acgtttattc tattggctat
     6961 atgaaacatg atgaggatta ctccaggttc atgtcatatc tgaatttgtt tatctttttc
     7021 atgctgttgc ttgtaatggg aagcaactat gtagtaatgt ttgccggttg ggaaggtgtt
     7081 ggactttgct cttatttgct gataggcttc tggtttaaaa acaacgacta caataaagct
     7141 gcaaacaaag cattcatcat gaatcgtatt ggtgatttag gatttttact tggtttgttt
     7201 ttaatgtatc aaacattttc atcattagaa tttaaagatg tttttgctgc tgctggaaat
     7261 tatccttcag gtgacgctac gctgactgca attgcattgt ttctgtttat tggtgcagta
     7321 ggaaaaagcg cgcaaatacc actctatacc tggttgcctg atgccatggc aggacctaca
     7381 cctgtatcgg cattgataca cgctgcaacc atggtgactg ccggtattta tatgattatt
     7441 cgaagcaatg tgatttatac gcttgcacct tttgcaggag agattgtggc tgttatagga
     7501 ttagtaactg cattgatggc agcatcaata ggtctgaaac aaaatgacat taaaaaggta
     7561 ctagcctact ccaccgtaag tcagttaggt tatatgtttg ttgcattagg agtaggtgct
     7621 tataccactg ctttgtttca cgtagtaaca catgcatttt ttaaagcatt actcttcctt
     7681 tgcgcaggca gtgtaattca cgccatgagt ggtgaacaag atatcagacg tatgggtggt
     7741 ttgaaaaaat atttaccgat aacacatttt acttttctga tgggagtatt agcgattagc
     7801 ggtgttccgt tgttcagcgg attcttctct aaagatgaga ttcttgcagt aacctggact
     7861 tattcaccgg tgatgtttac cgtattagct atcacatcag tactaactgc aacctatatg
     7921 ttccgtttat actggttaac tttttacggt gattttcgtg gcacacatca tcagaaagaa
     7981 catcttcatg aatcgccttc gagtatgact ataccattaa ttatcctcgc tgttttctcc
     8041 gtaattggtg gttatattgg catgccggaa tatattaatg aaaaattagg aacgcaccac
     8101 gtgtttacgc agtttcttaa cccaataatt tattctccaa aggaatttat tttatcacat
     8161 acatttgaat tcactttatt gataggcact ataatcggat tagccataat ttatgttatg
     8221 acaaaaaaca tgtatgtcag agataaagtg gtgcccgaag atgacagtca aacaaaatca
     8281 ttccaaaaag tacttgccaa caaatattat attgatgaac tgtatgaatt gataatcaga
     8341 aaaccgttag aagcatttgg cgaattgttc tataaaattt ttgacattag tatcattgat
     8401 ggaattgtaa attttatccc acgagccaca aacctgactt cttccgggtt gagattatta
     8461 caaaacggaa gtgttggatt ttatttggtg agtatggtca taggaatcat tttgattttt
     8521 gtatttaaaa tgctgctgta gatgaataat ttattactca taattttatt tttaccactt
     8581 gccggtatgg cattgtcgct gctgccttca cgcgaagcga caggaaaaac agcattagta
     8641 ttttctctca tcagtctgtt gctttctgtt tgcttgttta ttcaatttga cccgaatggc
     8701 ggcatgcaac aagttttgaa ttatgcctgg gtgcctgatt tgggaattaa ttttaaaata
     8761 ggtgttgatg gtatttcgtt gatgatgata ttactgacca acgtactatc tccattaatt
     8821 attctatcat cttttggtcg gagaatagaa aatcccaagg gtctttatgc actcattcag
     8881 ctgatgcaat ttgcattgat tggtgtattt atttccttag atgcattttt gttttacatt
     8941 ttctgggagc ttgcactatt gccaatttat ttcatctgcc tgttgtgggg tggcaaagac
     9001 agaatacgta tcacattaaa atttttcatc tacaccatca ccggaagctt attaatgctt
     9061 gtaggtttaa tctatctcta tctgcaaaca ccgggaagcc acacattcga tattcaggcg
     9121 ttttatgatt taacgctttc atcacaacaa cagtcgttag ttttctggtt gatttttgtt
     9181 gcctttgcaa taaagattcc tgtatttcca ttacacagtt ggcaaccgga cacttatact
     9241 gatgcaccaa cacaaggcac aatgttactt tccggtatca tgctaaaaat gggcctttat
     9301 ggtatattaa gatggctgct gcctgttgtt cctgctgcat cagcagataa cgctttcatt
     9361 gtaatgttac tgggaatcat tggtgttgtt tacgcatcgt gtattgccat cgttcaaaaa
     9421 gattttaaaa ggatgattgc ttatgcatcc attgctcacg taggtttaat tactgccgga
     9481 ttattcacat taaaagtaga agctcttgaa ggtgcagtat tccaaatgct cagtcacggt
     9541 attaatgctg ttggtttatt ttttattgcc gacatgctga tgagcagatt aaaaactcat
     9601 aacatgagtg aaatgggtgg cattgcaaat aacaattcgc tgtttgcagt tctgtttatg
     9661 atagtattat tgggaacaat agcgcttccg ttaaccaatg gttttgttgg agagtttctg
     9721 ttgcttacag gaatttacca gtttaaagct tggatggcat tatttggcgg attaactatc
     9781 atactcggtg ctgtatatat gttcagaagc tatcaggcta tcatgttggg tagccgtcat
     9841 ctgaatgaag gagagtttca acccatgaca ttgagcgaaa aaacagtttt aggaacaata
     9901 gcattcttgg ttattttatt aggtgtgtat ccatcttttc tgatgaatgc agcagcacca
     9961 agtataaaat cgctattaat gattattaat gcaggataat gtttaaaaat aaattaatct
    10021 cattgtgaat accatcgtta cactatctat agtcggcatt gcagccatgc tttcagaagt
    10081 ttttaaactg aagcgcataa tgtattcgct ggtaattgcc ggtttaattg ttgcattagg
    10141 tttctcaata aacgactgga ctaatttcgg ttcatacttc aatcaaatgg taatggttga
    10201 cagtttctct gttgtctttt catcactcat gattgtaaca acattgctgt ggatgctcat
    10261 gtcgcgtaat tacttcaaca gtgagagtag tatgagtgaa cattttgcac tcatcgtttt
    10321 tgcacttacc ggtggtgtaa tgatgactac tttttctaat atgtcaatcc tgtttcttgg
    10381 actggaaata ctttcttttt cactttacat tatggcagga agtgacaaaa ccaatatcaa
    10441 gagtaacgaa gctgctatga aatattttat catgggttcg tttgcaacag gatttttact
    10501 ttttggtatt acattgattt atggtgcaac atcttcgttc aacattcagg aaattgcaaa
    10561 ctatacacaa caaaacaaag atgcactacc tgcttttttt aaagttggtg tgttgatgat
    10621 attgattggt ttatcattta aaattgctgc agcacctttt catttctgga cacctgatgt
    10681 gtatgatggt gcaccaacat tagtgacagc gtttatggca tcggtagtaa agattgcagc
    10741 ttttgcaatg atctacagat tatttgtttc atgcttcata actatctatg atttctggac
    10801 tccggtattc gctgttctat cagccataac catgctggca ggaaacatca cagcggttta
    10861 tcaaacaagt ctgaaacgca tgcttgctta ttccagtgtt gcacatgcag gctacatgct
    10921 catggcatta gcagcaccgg gtattgcttc taaaaacgca ttgttgtttt atctggcagc
    10981 ctattcactt tctgcattag gggcatttaa cctactttat tctgtacata aagttacagg
    11041 aaacgaagga atcaaagctg tgaaaggttt gttttataaa caccctgtat cagcaattat
    11101 gctgactatt tgtatgctaa gtatggcagg tattccaccg gtggcaggat tttttggaaa
    11161 atattacctg tttaccgctg cattaaaaga gaatcttctg tggttagtgc tcattgctgt
    11221 tttaagttcg cttattggaa tatattatta cttcaaagtt ataattgcag cctttcaaca
    11281 gcctgagaca gaaaatacag gctactgcga aaaaccatct gaccttgcca tattaggcat
    11341 ttgcgcagtg ctatccttgt tgcttggcat tgctccacaa ctaatttcag gattgctctg
    11401 attatttatt ttacttacag agcaacagca atcaaataaa ttttattttt tcttcccatt
    11461 atacttgatg ccattaagta atactataaa gtatgctcaa atgtgctgcc atcaatacta
    11521 aagaatttta cttgccggtg gcgcaaaggc gcgcgacctt accggtgagg ttataaaaac
    11581 gtattaaccc atcaatgctt tttattattt caaagtcccc tgaaatgcgg gagactttga
    11641 aagtacagaa aattaattta atttgtctct attataattt tcccaatata cattttttct
    11701 ctatcgctaa cttttacaaa ataaattcca tcaaccattt tatctatgca gatttttttt
    11761 aggttagaat tatttatatt atctgaataa atcctatctc caagcacatt ataaatttct
    11821 atttccgcat tagcaaaatt atctgagctt gaaatagtaa atattcctgt ggaggggtta
    11881 gggtaaacaa ttatattttt tgtttctgta aagaaatcat agacgccaac attacccata
    11941 catgctacgc taacatcacc aaagcttgat gtaaatgcgt aaactggaaa ttgataaccc
    12001 tcattaataa cctgtataat cagattattt gacattatgc taattgtatc gtttagcgtt
    12061 tgtggattaa tctcaaagca gccaccacta ttccctataa aatcagtttt aatgatataa
    12121 caatcattat accctaatcc aaaacttgac gtgaacccaa tcatgataca accactgtcg
    12181 gatgataaat ccatatcgta gcattgttca ctacctgaac cgccataagt ttttgaccac
    12241 agcaaaaatc ccaaactatc agtttttatt aaataggaat cccaattatt catgttaaaa
    12301 ctttctgtat agcctgcaat taaaaatcca ttgtcaggag tttgttttat cctaaatcca
    12361 taatcattaa aattgcctcc aaacgatttt ctccaaagca aattgcctat gctatcaatt
    12421 tttatcagac ttacgtcaaa gacaaatagt ccattttgaa aattccaccc tgtaataaca
    12481 aaacattgat cattggtttc ttgaattgaa tgtccaataa agccattccc tggtgctgaa
    12541 agattttttg cccaaataac attgcctgca gaatttatct ttattatatt ggtagagtta
    12601 gaaggataat ctgaactacc aatcaaaaca aagcaactat cgtgggtttg tattatttga
    12661 gtacaataat taagaccact agcttgataa acttttgccc acagtagatt tcccgatgta
    12721 tcaacctttg taacataaaa ctgactataa taattaaata caaattcctg tgaggttccg
    12781 gcagctatgt aatttccatc ataagcctgt aaaacagatg tactagaatt tatatatcct
    12841 gaccgaataa tgtttgtgtt ccataaaatt gttcctgaag aatctgtttt aaaaataccg
    12901 aacccttggt tagatgcccc tcctgttccg gacataccac ctataataaa cccgtgatcg
    12961 tcagttttag caattgcctg aacactattt gacaaaagtg tctttgtcca taatgtatca
    13021 ccagctgaat ttaatttagc aagaaagcct ccacccgaag cagagcttac ctggcttcct
    13081 ccaatgataa atccaccatc acctgaatta ataactgtca atccataatc actgttatta
    13141 cctccatagg ttttttgaaa aacaaactgg ccataagaaa taaatgttcc gaaaataaaa
    13201 agcagtgtag aaagtttctt attcataagt atggcattat tataaataca attctatttc
    13261 cttccagcaa aattaatgtt aaaagtaaag tatttccctt actcaagtgt atcactttta
    13321 cgacccattt tctccaggag aatctcttcg catttaaaaa gaactagggt aaccaaaaac
    13381 agaactactt gaaaatatga atttcacctg tcatgggcgc gcctttgcgc ccatgacagg
    13441 tgaaggtaaa agcttatagc tctctaattt tcgaaactaa aattcaacga taactttatg
    13501 tgtagaagat gtacgtccgt cagttacttt aataaaatat acacctttag gtaaattgct
    13561 taaatcaact tccacagatt gttctttgtt tgtttgttga aaattgactt tctgaccaag
    13621 tgtattaaca acttctaaca aagcattggt actgctaata ttgctaagta aaaatttacc
    13681 tgaagaagga tttggcgtga tggttatttc tgatggcgct gctatctggc ttatacctgt
    13741 tgttcctgaa attggttgat agcgcaatac cggtgtttgt gcagttccgt caatagttgt
    13801 tatgccataa gtaagcaaag gttgtttgat ggtatcatga aaccaataat aataatcatc
    13861 agaaaccaaa taatctataa caggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnttagat gcgctgctat accagccaat acggttgatg tcatttgcag tattctgata
    14041 ataaaaataa ccggttgctc cactctcgcc acgtgccagg gtagcgccag gaaaagaatc
    14101 aacaccattt cctgttatag ttgccgggtc gtcatagaaa aaaaggtagt tggtgcctgc
    14161 attcataagc agtgcattat cccaaagtgt attggtaaca ggtcctgtac cgttttgatt
    14221 gaacccaaat gtgttggcat caacatagcg tatggtatca ccaatagcag gtaactgatg
    14281 tgttgcagag atggtaacga gttgtgcact tgttttaatt gcagcagcac aaaatagtgt
    14341 ggcaatgagc gtaaatgttt tattcatgtt atcatttttt tgtgaagcaa tattaaaaaa
    14401 atatttcaat gttgcatgaa ttgctatcaa tagtctttac tacattttta aacatccggc
    14461 aatatgctaa atgctatcca taaaaattcc atacttcaaa ttctgatttc cctgacgaga
    14521 gtaatgagat acaaactcca caaaggtttg ctgatgcaaa tcattttata tttcatctgt
    14581 ttccgccatc ttgcacacct ttattttaat cacaaataat gacagaaata atgtcgcctg
    14641 caggctcgtt cgagtcgctg caatctgcta tacaaggtgg tgccgatgca gtttattttg
    14701 gaatagaaca actgaacatg cgcgcacgtg ctgcaaacaa ttttacactg agcgatcttg
    14761 ctgaaataaa aaaaatatgc actgcaaaaa atgtcaaaac ctatcttaca ttaaacaccg
    14821 taatgtatga tcacgacatt atgctgatga aaaaaattat tgatgcggca aagaaaaatg
    14881 atattgatgc tgtcatagca tctgaccatg ccgcactgat gcatgccaga aaaattggta
    14941 ttccggtaca catttcaact caggccaatg tcagcaatat tgaaagtgta cgcttttttg
    15001 cagattatgc agacactatt gtgttggcac gtgaattaac attgaatcag gttgctgatg
    15061 ttgtgaaaca aataaaacgt caaaaaatta aaggaccttc gggcgaattg ataaagatag
    15121 aagtatttgc acatggtgcc ttatgtatgg ccgtttcggg caaatgttat ctgagtcttc
    15181 actctgacaa tgcctctgca aaccgtggtg catgtattca gaattgcaga cgtgaatata
    15241 ttgtacaaga taaagaaaac gaaattgaac ttgaaattga caatgaatat atcatgtcgt
    15301 caaaagattt atgcactatt gattttattg acaaactcat tgaaacgggc atcagtattt
    15361 tgaaaattga aggccgaggt cgttctgccg attatgttta tactacaaca aaatgttata
    15421 aagaagctgt tgaaagttat cacaatggca gctataatgc aacaaaagct gaacattgga
    15481 aaaaagaact ggcaacagtt tttaaccgtg gattctgggg cggttacttt cttggaaaaa
    15541 ctatgggtga atggagtaat acaggaggct cgcaggcaac taccaaaaaa atttatgtcg
    15601 gcaaaggggt taaatatttt ccaaaaagta aggtgggaga gtttaaactg gaatcaggaa
    15661 cgctttctgt tgacaatgat ataatgatta ccggttcaac aacgggaatt attttaacca
    15721 aggttcagga attgcgaatg gatgatttgc cggttacaac agtaaaaaaa ggcgatgttt
    15781 tttcaatgcc attagctgaa aaaataaaat cgtccgataa attatataaa atcgttaatg
    15841 attgaaatag tacaatacag aagcaaatgt attggatgca atgcctgtgt tgaagcttcg
    15901 gcaacacgct ggcgtatgtc gcgcaaagat ggaaaatgta cattggttgg tggaaaaaat
    15961 aataaaggta tttacatcac tgtagttaat gatgatgaat atgaacaagc attgcaggca
    16021 gccaaaaact gcccggtaaa tattattaag gtaaaaaaga aataaatgga tgcacaaata
    16081 gaaaacttta ttggttcaca gaagaatatg acactatgca cctcaattaa caacacacct
    16141 gaatgtgcaa attgtttcta tacatttatg ccggacggtg attatctgat ttttaaatcg
    16201 aaaaaaacta ccggtcatgt ttctcatgca cttgtaaata ataaagtggc cggcannnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaaa aactaccggt catgtttctc
    16381 atgcacttgt aaataataaa gtggccggca caatattgcc tgacataagt aagattggaa
    16441 gcatcaaggg catacagttt tcaggaactt tccttacgcc acaagatgac atgctggagc
    16501 aggcgaagaa aatttattac agtaaatttc ctttggcatt aggaatttcg ggcgacctgt
    16561 ggctaataaa attaacctac attaaaatga ccgataacac acttggcttt ggaaagaagc
    16621 tgctatggga gaacaagaac tgatttaaaa aatcatttat attgcttctg cagtttcgta
    16681 agacaaaaca tcaggacaag tacaaactaa attcctatcg ccataagcat tatcaacccg
    16741 cttaaccgat ggccagaatt tatttcctgc aacccaaggt gccggaaaaa ctgctttctg
    16801 acgtgagtag ctatgcttcc attcatctgc tgttgcttcg agtgcagtat gtggtgcatt
    16861 cacaagcata ttatcttcct tgctgtattc gccattgatt acttccataa tttcatgtct
    16921 gatagcaatc attgcatcac aaaaacgatc tatctcttct aatggctcgc tctctgttgg
    16981 ttcaaccata agtgttcctg caacaggaaa tgctactgta ggagcatgga aaccataatc
    17041 catcagtcgc ttggcaatat cagttacatc aatattggca acagctttga atgcacgaca
    17101 atccaaaatc atttcatgag cacaaaaacc atttttccct ttatataaaa caggataatg
    17161 tttttctaat cgtgctttaa tatagttggc attgagcatt gctatttctg ttgcacgttg
    17221 tacacctgac ttacctagca tttttatata tccataacta atcaacaaaa ttaatgcact
    17281 gccccaagga gctgcggcaa cagcatttac gcccttacgc ccacctgtag gcacaacagt
    17341 atgcgaaggc aaaaatggtg caagatgttc tgctactcca ataggtccca taccggggcc
    17401 acctccacca tgtggaatgg caaatgtttt atgtaagttc aaatggcaaa catcagcacc
    17461 tatcaatgcc gggcatgtta aacctacttg tgcattcatg tttgcaccat ccatataaac
    17521 ctgaccaccg ttggcatgta ctgctgctgt tatctgttta atattctcct catacacacc
    17581 atgggttgat ggataagtaa ccatcaaaca ggataaattt tcctgatgtt gttctgcttt
    17641 cagccttaaa tcactaaggt caatatttcc atcatcatca catttaacaa caacaacctt
    17701 taatccggca agtacagcac tggcaggatt ggttccatgc gcagactgcg gaatcaatac
    17761 tacattgcga tgattctgtc ctctgtcatt atgatatttc tgaataacaa ccaaaccggc
    17821 atattctcct tgtgcgccac tgttaggttg taacgacact gctgcaaatc ctgtaatctc
    17881 tttcaggtaa ttttccagtt ctgttataat atgcgcataa ccttgtgcct gttcaaccgg
    17941 agcaaatgga tgcatgttgg caaactcagg ccagcttagt ggctccatct cgctggtggc
    18001 attcagtttc atagtgcagg aacctaatga aatcattgaa tgattcaagg caagatcttt
    18061 attctcgaga cgcttgatgt agcgtaacat ttctgtttcg ctatggtgtg tgttaaatac
    18121 tgcattggtc agaaattctg aactacgtaa tagcgtagca ggtatagcat taccattaag
    18181 acttactgca gcaattgact ttttaccaat acttgccgca aacacacgca aaatgtcttt
    18241 taaatcttcg tcagaggttg tttcatctat actaacacca actgaattgc ccttgtaacg
    18301 gaaatttatc agattggctt cagccatatc gcgaacactc tctgcatcct ctacctcaac
    18361 agtcaatgta tcgaaccagg agttattcac cacgttaaat cctgctttct ttaaactctc
    18421 tgcaagtgca gtagtacgtt tatgtacagt agtagcaatt gccttcaatc cttctgcacc
    18481 atgatatact gcatacattg aagccatcac cgctaacagc acctgcgcag tacaaatatt
    18541 gctggttgcc ttatcacgtc ttatatgttg ctcgcgtgtc tgcaatgcca tacgcaaagc
    18601 cggattccct tctgcatcca cactcatacc aataattctt cccggtagat ttcttttata
    18661 aacatcatgt gttgcaaaat aggcagcatg cggaccacca tatcccattg gtacaccaaa
    18721 acgttgtgta gatccaacta cacaatcagc accccactca ccgggaggtg ttaacaatac
    18781 aagacttaaa atatctgccg ccacacaaac acgtacatca tgcctgtgac acatgttaac
    18841 aatattttta taatccaaca cattaccaat tgcatcagga tactgaagta atgcgccaaa
    18901 aaaactgtca tcaggcgaaa acaattgttc atcgccaaca accaattcaa tgcccaatgg
    18961 tttactgcgt gtacgcaaca catctatggt ttgaggaaag cagttttcag atacaaaaaa
    19021 cttaactgca ttttttttct ctgcctcttt agagcgggtg taataaaaca tcaccattgc
    19081 ttcagcagca gcagtagcct catccagcaa cgatgcattt gcaatctgca tgcctgtcaa
    19141 atcgcacacc atagtctgaa aattcaacaa agcttccatc ctgccctgtg caatctctgc
    19201 ctgataagga gtataagctg tgtaccatcc aggattctcg aaaatgtttc tttttataac
    19261 accaggcaca atggtattgt aataccccat gccaataaaa tttttaaaaa gttgattttc
    19321 cgatgctact gctttcaact catttaaata ttcaaattcg ctcatcgcct cgggaaggtt
    19381 taatggtttt tgcaagcgaa tcagttcagg aataatttta gcaataagat cgtttacgga
    19441 ttttacacca atggtggcaa gcatgtgttt aacctgctta tcatcagggc caatgtgcct
    19501 gtcggcaaaa gaatagtaca tgtactttgg attttggcgc aaaagtaaat aattagaacg
    19561 tcaaaaaatg aattcctgta tgccttcatc atgatttaca tcaagcaacg tactcttttt
    19621 agcacaaaaa ttactaacac atgttgatat tccaatattt ctaaaaatct gggcttgtca
    19681 ccgcctgtgg cgctgtcgga ctatacactg caagtccgct ccttcctgcg gaagtatgcg
    19741 ggctttacgt tgctatccct caagcaaaaa acatctccta aacaaaaact tttctcctcc
    19801 gattcgatta tccatattac aacacattat caaattttta acaaatgctg catattacat
    19861 acatttttta tagttttgaa tcaaataact gatatgacct tacgtcactt tataacatcc
    19921 cttatttttc ttttcagcgt tacaacatta agcgcacaag acaccttatt gctccttaat
    19981 ggtaagacca aacccgtaaa actttttaac ctcaacgacc ccgactgggt gagatatgta
    20041 aaactgcaag gcagttcgcg tgttaaaaaa gttgatattt ataaagtatt ctccattaaa
    20101 aaagctgatg gcacagagca agttctttat aaacccgaca gcacatcaga cgacccggat
    20161 attccttggg tgcgcgattt tattgcagga cagcaatatg gaattctgca taaaagagaa
    20221 cgttttaaca aacacgataa tcaatggcac cgcagactaa actttactga agtaggcgga
    20281 atagctgcag gcggtgcagg aagcctttta actttttatg gtattccggt acctgcaatt
    20341 tatgccattg ctgtagggcg gcatagccct gcattaccca acgctccaga tatttcacct
    20401 gaaatgaaaa acagcgaagg atttatttca ggctatcaaa agcaaagacg taatcagcgg
    20461 atcagacaag gatttatttc tggtatgata ggttttgctg ttggcattgt tacattcaca
    20521 cttatagaac ggaactgatt gttcaggaag attgctcata aagctgtttc ctataatatt
    20581 ctcattgctc tggcagcaag tgcagcaagt catttaacca ttctcagaat aaacgaacaa
    20641 aacaatgtag tgctcattgc aactatattc tttgctacac tggctatcta taattttcca
    20701 cgtctgccat tgcagatgcc acttacttca cggacatggg tatgtctttt ttcgattctg
    20761 gctacagcaa tattaatttt caactataat tatattgcca taaatctctt tttgacagct
    20821 attctggctt gtctcattta tatgatgcct tttaattata agggcaaacg catcaaagga
    20881 attcgaaata tctttctgct taaaaatatt tttcttgctt taagttgggc tttaattact
    20941 gtgctcttac cgatggctga attagggcat gaatcaccaa taattaaaga ggttttttta
    21001 ctccgcttta tttatgtttt cggcatttct ttattgtttg atattcgcga cactgcaaaa
    21061 gacaaaaccc gcaaccatct gacacttcct gtggttttag gtacaacagt aacccggtat
    21121 attgttattg catctgcagc attatttatt ttaattgttt ctgttcttta tcgtcatgag
    21181 cattttagtg cacctatggc aacagcattt ataataagca cgttactttt agtcattgct
    21241 gctatcacag cgccaccgtt ttcaaacttt aaattctatc aggttggggt tgacagtttg
    21301 ctaatcgtac aatggttatt ggttgttgtt gctgtaaaat tttattaatt tctctctaca
    21361 ccatcttcgg tttcaaaatc ctgttttaat ttttttggta atttaatctt aatcatgtag
    21421 gtttgaactc cactaaaatt caagattaaa gttatgtcca aaggaataca tgtaaagaaa
    21481 cctctcgata gtttattagc tgaagccgaa gacagtgaaa aaggtttaaa aagaacactt
    21541 actgcaaaca gtcttatcgc actcggtatt ggtgcaatta taggtgcagg actatttgtg
    21601 cgcactgctg ctgctgctgc aaacaatgca ggaccggctg ttacaatagc cttcgttatt
    21661 gcaggtttgg gttgtgcctt tgcaggattg tgttatgcag aatttgcaag catgattcct
    21721 attgccggta gtgcctatac ttatagttac accaccatgg gtgaatttat tgcatggatt
    21781 ataggatggg atttggtgct tgaatatgca ctcggtgcag caacagtaag tattgcctgg
    21841 agcgaatacc tgaataaact attaaattat ctgggcatgc acatacctta tgaatggagc
    21901 cattcccctt ttgaacatag tttagatggt gtatatggta ttatgaatgt tcctgcactt
    21961 ttaatattag tccttctttc tttgctatta atcagaggaa ctcaagaaag tgcttttgta
    22021 aataatgtta ttgttattac caaggtagct attgttgttc tctttattgt ttttggctgg
    22081 cagtttatta accctgctaa tcacacacct tatattcctg ctgcaagtac ctttaccgac
    22141 cacatgggcg tgccacatca ttatggagga attttaggaa ttctcggtgc tgccggagta
    22201 gtgttctttg cttttattgg ctttgatgca gtgagtacag ctgcacagga agcaaaaaat
    22261 ccgggacgtg atatgccaaa agggatttta ggttctttgg ttatttgtac tattctttat
    22321 attctgtttg cccatgtgct tacaggagtt gcctcagtag atgatttcag aacgcagggt
    22381 agagaagcct ctgtaactta tgccattcaa acctatatgc atggatatga ttggttagct
    22441 aaacttgtaa ctgttgcaat tcttgccgga ttctcctctg taattttagt aatgctttta
    22501 ggacaatcac gtgtatttta ttctatgagt cgtgatggat tgttgcctaa agttttttct
    22561 gaactacatc ctgtttaccg cacaccttat aaatcgaatt taattctatt tgtttttgtc
    22621 ggactttttg ctgcatttgt gcccggagat attgttggtg atatgacaag cattggaaca
    22681 ttgtttgcat ttatgcttgt tgcagccggt gttatcatta tgcgcaagac gaatcctgat
    22741 gccaaacgta ccttcagaac accgttagtt ccattggttc ccatcttagg aattattgtt
    22801 tgcggagcca tgatttttgg tctgggttgg ccaaattggt cacgcttatt aatgtggctg
    22861 gctctcggtt tcgttattta ttttggttat agtgctaagc acagtaaatt acgtaatccg
    22921 aaataaaata ctttcttaaa aattatatgc acaacgacaa accacacctg caccgttcat
    22981 taggactgtg ggatgccacc atgctggttg ccggttcaat gattggctcc ggaattttta
    23041 ttgtaagtgc cgacatgtca aggcatacag gttctgcagg atggttactg tttttatggt
    23101 tgcttagtgg tgttatcaca ctaatagcag cactttgtta tggcgaactg gcaggtatga
    23161 tgccgcaagc cggaggtcag tttgtttaca tcagacgggc atggggaaac tgcttggcat
    23221 ttttatacgg atggacagta tttatggtca tccaaacagg agttatagca gcagtggctg
    23281 tagcatttgc aaaattcact gctgtattct ttccatattt taacacgggt aatattttat
    23341 ttcaggcagg aggaataagt atatctgctg cacaaatgct ggctattttt ctcattgctg
    23401 tactcacatg gttcaacagt tatggtattg aaggaggtaa aaccattcaa aacattttta
    23461 catcagcaaa gctacttgcc ctctttatcc tgattgtagc cggactttgg gttggactaa
    23521 aaaccaacac cttatcagcc aacttcgaca atgcgtggag cgttaattca atgattaaaa
    23581 ccgattccag ttggattgag caacatatca gtggctttgc aataattcta atgcttggca
    23641 ctgccatcat aggctcatta ttcagcagcg atgcatggaa caacgttaca tttattgccg
    23701 gtgaaataaa agacccaagc cgcaatattc cacgaagcct catgttgggt acagcgatgg
    23761 tgactttact ttactgttta gccaacattg catatcttgc attattacct ttaaaaggca
    23821 gtattgacgc tgcaacagtt gcggggcaag gtattcagtt tgccggtggt gataccgatc
    23881 gtgtaggtac agccgcagcc tccatgattt ttggtgactc aagtgtttat atcatggccg
    23941 cactcatcat gatttcaacc ttcggatgca acaacggtat tatcttaagc ggtgccagag
    24001 tttattatgc aatggcattg gagaatctat tttttaagca ggcaaaaaaa ctcaattcaa
    24061 aaggtgttcc tgcaatagct ttgtgggtgc aagcaatatg ggcaagttta ttatgcctta
    24121 gcggcaccta tggtgattta cttgactaca ctgtttttgc ctctctcctg ttttatgtta
    24181 taactatttc cggtattttc attttacgta aacgtgaacc tgaagcagaa aggccataca
    24241 aagtgttcgg ttatccgttt ttaccagcac tctatataat tttggcacta tcaatttgct
    24301 ccattttact atataccaag acagcagtgg ccggtaaggg cttacttatt gtaatactcg
    24361 gattacccat ctattatctg tttaaaagac gcaatagtta aatagatttc tcagctattt
    24421 aaaagctacg tatattcgta gttcaaaata taattgtcga acaaatgaga aaaatctcta
    24481 cactatttgc tgcaatgtgc atcacagcca ctgtttcagc acaaacacaa cgccttgttc
    24541 tcttcgagga atttacaggt gaaacctgcc cgccatgtgc acaaattaat ccaacactca
    24601 atgaaatgtt gaatgatgaa gcagacaaaa ttgtttctat taagtatcaa aacaacatac
    24661 cgtctgcagg tccacgattc tggccttatg cacaaacgga tattgtagca cgtcagacat
    24721 attacagcaa ttcttattcc cctcagggaa ttatggatgg caatcagttc aacgataatg
    24781 caggcgcttt agatcctgtt atcatcaacc ctcgttatgc aacaccatca ccatttgaag
    24841 tcagagttgc acatactttc agtgcagcca atgacactat ttatgcatcc gtaataataa
    24901 aagcagcaca aaattataac ggctcaaact tggtagcaca gattgcagtt accgaacgtg
    24961 atatttttgg atacacatca cctaatggtg aatcgcacta cgagggagta cttcgtaaaa
    25021 tgttgccttc tgcatctgga acaccattgc aacaatcatg gaatgcaggt gattctgttg
    25081 ttttaaatta ttcatggtac attacacctg ctgcactttc atctcctgtt tattatcagt
    25141 tatcagttat tgcatttgtg caagagaata gcaataaaga agtattgcag gcaggtttca
    25201 gccgtgcaca agtccctatg gatccaaaag caaaagctat aacaggaatg caggatgtta
    25261 catgtaccgg taatattacc ccaactttta cgattgcaaa taatggcatt actgacattt
    25321 caactttaga catacaatat aaagtagaca ataaccctgc acagacatat tcctggatgg
    25381 gattgttacc tagttttaca aatactacca tttcattgcc accaattccg gtttcaggaa
    25441 acggaagtca cagcattact ttaactacaa caaatccgaa tggtggtgtt acagataata
    25501 atatgttaaa caatagcaaa actgcatact tcggagttcc tgtggctgct gttgcaggta
    25561 atgttttaga ggattttgcg ctatcaactt ttccaccggc taactggatt cgtgtagatc
    25621 agaatggaaa caataccgga tggttacgtt cgactgcagg aaatggctct gctaaaattg
    25681 atttttacag cagctttacc ggtcacattg acaaccttta tgtgtatcct gttgacatga
    25741 gtggtgcctc tgtagcaaca atgacctttg atgtatcaca ccgtcaatat agtgctgcct
    25801 atattgatgc aataaatgtt caggtatcta cagattgcgg actaacctgg aatacagaat
    25861 gggctaaggc aggtgctgca cttgcaacag ttacaggtta tcagaccggt gcatttacac
    25921 caacaacagc agcacagtgg agtgctgaaa ctgtgaacct gaatcaatat gcaggtaaca
    25981 ataatgtact tattcgtttc aatgctgtga gtggttatgg caacaatgct tatgttgaca
    26041 atgtgaatgt ttcgttatca acaggaatta acgaagttgc gaatactgta tttgaattgt
    26101 atccaaaccc aacaacaggc attgtaacta tcagaaccaa gacacaaaat tctgattata
    26161 aaatccattt ctctgacatt tcgggaaaag aagtttttgt aaataccctt attgaaggca
    26221 atattataaa agcagatttc aacacatgca gcaatggaac atactttgcc gaaatacaaa
    26281 caccaaacgg aacagaacgc agtaaatttg ttttgattaa agattaattt ttttttcgta
    26341 aaaaaattgt aataattacc actaagccat cactgaaata ctgatggctt tttgatttta
    26401 tagcaaagaa acaaacccgt gttttgagtt tctttagctt atctttttac ctttgcactc
    26461 tcaaaaaatt taatttaaaa tcaaacttag ttatgtcaaa aggacccata tcgcaattta
    26521 tgcagcatca ctatcgtcat tttaatgctg ctgctatgat ggatgcttca aaagcttatg
    26581 ttacacatct tagcgaaggt ggaaaaatga tggttacact tgccggtgct atgagtacag
    26641 ccgaacttgg aatttcactt gcagaaatga ttcgtcagga taaagtgcat attatttcat
    26701 gcaccggagc aaatcttgaa gaagatatca tgaaccttgt agcacataat agttataagc
    26761 gagttcccaa ttatcgtgac cttactcccg aacaagagcg tgaattatta gacaaccatt
    26821 ttaaccgtgt aacagacact tgtattccag aagaagaagc tttcagaaga ttgcaaaaac
    26881 atattcatca gatatggaaa gaagccgaaa ataaaggcga gcgttatttt cctcatgagt
    26941 acatgtataa aatgctattg agtggcgtat tggaacaata ctatgagatt gatccaaaaa
    27001 actcatggat gcttgcagca gcacaaaaaa atattccaat tgtagttccc ggctgggaag
    27061 acagcaccat gggaaatatt tttgcatcct acgtcatcaa aaaagaaatg aaagcaacaa
    27121 ccatgaaaag cggtattgaa tatatggtat ggctgagtga gtggtatcgt caaaattctg
    27181 gtggaaaagg tgttggattt tttcagattg gtggtggcat tgccggtgat tttccgattt
    27241 gtgttgtacc tatgatgtat caggatttgg aatggcatga tgtaccgttc tggagttatt
    27301 tctgtcagat tagcgacagc acaacaagtt atggttctta tagtggtgct gtgccaaacg
    27361 aaaaaatcac atggggcaaa ttagatattg acacaccaaa atttattgta gagagtgatg
    27421 cttctattgt tgcaccgctg atgtttgcgt ggatattaga ctggtagtat tgtactatga
    27481 atacagccgt taaatacacc ctggcaggca ttgccgcagt tattttagga tttataagct
    27541 taggtattat tcatcctgaa atagaatacg aatcaagggt acagattaac gcacccattg
    27601 caaaagcatt tgatattttt acagacactg caatgatgaa acactggatg cccgggtttg
    27661 tgtcattcaa accattagaa ggagaaatac acactaaagg ctctaaacaa ttattggttt
    27721 taaatctcaa caacgaaaaa atagagatta ccgaaacgct tgaagattat agctatccga
    27781 aattatatca atttcatgca caaaacacat tgctgaattc aaccaacacc ataatttttg
    27841 aaggtgattc tcttcagtgc aatatgacta taaaaacaaa agcaagtggc aacaacttta
    27901 tgctgcgttc tatgtttgta tttacagaac ccatctttaa acaacaacag gacagtacat
    27961 atacattgct taaaagaaca attgaatctc atttggataa tcacaaaatc aaccagtaat
    28021 tgtaactaat tatattttaa acaatcatta aattaataat cagacatgaa tagtagaatc
    28081 ataaactttc tcttgctatt attgatttca accactgtaa atgcacaatc agatgcagaa
    28141 ctgaaatcaa acctgaaaca acatatcagc atattagcca gcgataaatt tgaaggacgt
    28201 gaaccgggga ccaaaggcga acgtttatct tatgaataca ttacacaaca atttaagaat
    28261 attggtttac aaccattagg cagtaatgat tatttacagt catttgaatt ttctgcagga
    28321 acaaaaaatg gtgctaaaaa caaattgaat atagccggaa aaagtttgaa atcagatact
    28381 gaatattatg tgttacctta ttctgcactt caatcaactg catcgggtaa atcaataaaa
    28441 atcggaagcg gattgttatc aaaagaactt ggcagaaacg attatgaagg gtttactgat
    28501 ttgaaaggta aaattgcagt gatggattat gctactccgg aggcagataa tccacacagt
    28561 aaatgggctg aggcatcaga cttacgtaaa agaatagaaa ttgcagtatc aaaaggcatt
    28621 agtggattga ttatttataa ttcaaatgat tccataaaaa atcctgaaaa aaattcggaa
    28681 ataaaaatca cgccttttgt cattcctatt gtatttgtaa aatcaattga cgctgcaagg
    28741 ctaaaaaatg atcagcagat aactatagaa actgatattg aaaaaataat gcactccggt
    28801 cataatgtaa ttggttacat tgataaccat gcaggaaaca cagttttgat tggagcacat
    28861 tatgaccact tgggttatgg cggacatgaa tcactctata gaggtactcc tgctattcat
    28921 aatggtgctg acgacaatgc aagcggaaca tcaacattaa ttgaactggc aagaaaatta
    28981 aaatcatcat catttaaaaa atacaactat ctgtttatcg ctttttcagg agaagaaaaa
    29041 ggactccttg gttcaaacta ttttgtaaaa catcctacaa ttgatttgaa gaaagttaat
    29101 tatatgttga atatggatat ggttggccga ttgaaacctg aagaacatgt tttacttatt
    29161 aatggtgccg gcacttcacc ttcatgggat agtttactaa ataatatcaa agtagattct
    29221 ctcagaataa aaactactgc ctctggtgta ggcccaagtg atcacacttc attttatctg
    29281 gaaggaattc ctgtactgca ttttttcagt ggcacacaca gcgactatca taaacccagc
    29341 gatgacgagc ctctgattaa ttacaatggt atggtttcaa tttttcatta tatgtacgac
    29401 ctgattgagc aaacaaacaa attaccaaag cttgccttta caaaaaccaa tgattcaagt
    29461 aatgaagatg cgccacgttt taaggtaaca ttaggtgttg ttcccgatta tgcttttgaa
    29521 ggtagcggaa tgcgcattga cggagttaca gaaggaagac cagctgcaat tgccggattt
    29581 ttaaaaggtg atgtggtaat acagatgggc gaagaaaaaa ttactgatat gatgagttat
    29641 atgaaagcac tgagtaaatt caagaaaggt gataaggtga aagtaaaagt tcaaagaggc
    29701 aatgaagttg ttgaaaaaga tgttacattt tgatgaaaaa aaagtatgac acagcgaacc
    29761 atacactttg aagaaaaaca atatttggga ttaaaccact atagtctgct gcgaagcatg
    29821 gtattggcac ttttttgctt tgtagcttac tttgtgacag aaaaccgtga aaacaatgca
    29881 gagttactct tctttggtgg tgtggcgttt atggtaattt cattactttc tatgtttatc
    29941 aaacacctgc atacacgggt ctatgatact tgcattgaac tggatggact ttggacaatg
    30001 cgtaaagtaa aaattgatct cagtacaatt gttgctattc aaaaaatccc gtacagcaac
    30061 tacattatga ataccccagt ttataacctg cacagaaatg gtagtattta tttttatact
    30121 tcaggtcgtt ctgcaataaa agttactgat aaagatggca tgaattatat tatcggtaca
    30181 caaaaacaag aggagttttt aagaattctt aatagtctta tcaaaaaata aagcggttgc
    30241 ctcattttag agacaaccgc tttcatcaac attgttttgc tactgtttta ctgcttagga
    30301 gtatcctttg gtttttccat ttcagtagcc ttgtgagcgt ctttttgagg tgactcttta
    30361 gttgcagctt tatcggtagg atgtgtagcc ggttcagctt catgctttgc aggtgcagcc
    30421 tgaatcgttg ccggtgcatc ttgctttacc tccttttggg gtgttgtggc ttgctcactt
    30481 tttacaggtt gctcttgtgc ctttttttcg gtttgtgcat tggcgcaata tgctgctgcc
    30541 actaccgcta atgtaagaac tgattttttc attgttattt gattttgaag aatttaagac
    30601 aaatataaat ataatcctaa atgctgcaaa ttagccgttt gcacggatgg cttctaccgg
    30661 atcgagttgt gatgctgagt acgctgggat gaatcctgaa accaacccta ttaaagcgga
    30721 aataaacaat ccaagcataa tattatgccc ggtaagactt agtggataat caaaggcatg
    30781 agaaacaaac atggctaaaa aatatataat actcaaaccg atcaatcctc ctatcaggca
    30841 aagcacgata gcttcaaaca aaaactgcat cagaataaaa taattctttg caccaagaga
    30901 tttctgaata cctatctgac tggttcgttc cttcactgaa acaaacatta tgttagcaat
    30961 accaaatcca cccactaaaa ttgaaaagcc tccgattatt gcccctgcca tattcagtga
    31021 gctgaaaata ttggtaatgt tattacttaa caaacttgac tggttgagtg caaaattatc
    31081 atcctccacc ggtcgcagtc ttctgatact tcgcatgatg gatttcaatt catcattagc
    31141 aaactgaata ggaacccctt ccttgacctt tgcttttatg aatgggtcta ttctgtcact
    31201 ccgtatatta acgaaattac gaccaaaatt caatggcaaa accacagtat tatccatctc
    31261 tgtctcaagc agacttttac cttcttcctt gaaaacacct atcacttcca ccttgcggcc
    31321 cataacttca ataactttcc ctaaagcggt acttgcattt ccaaaaagtc cgttagcaac
    31381 attactgcct ataatgccaa cagccctacc ggagttagat tctatttcag taagataacg
    31441 accggctgat aattcgaaag attttacctt atcaaaatcg tgcgacacca gggccacagt
    31501 tacattttct gcactattgt tcaaatattt aattgtttta ccactcagat atgcttcgaa
    31561 agtaaaaact tctatactct gtgaacgtgc agccaactga tgcatttctt ttacagaagg
    31621 tagcggacgc tgccagtatt tccaccaagg gtaatcgcct ccaaaggccc agggccattt
    31681 ctgaacatag atagtcttgt cccccaatga ctctacactg gtgcgtacat ttttttccat
    31741 ggcatcaacc actgtaaaaa cactaataat ggcaaaaata ccaatggaga ttcccaataa
    31801 ggacaaaatc gttctgagca tatttgccct caaagcatgc aatgcaaatg ctatgctttc
    31861 acctgacaat ttcagaaaaa tccgtaatcg gttcattgca acaaaagtat aaagatgtgg
    31921 tgatttactc tgaatttatt ttcagtttaa caaattttaa ttgatacact tacctatttt
    31981 tgccaactct ccatcaattt aaagaattcc gggaagtgaa gattaaaagt gcgcttattt
    32041 ctgtctataa taaaaatggc tttgaaccac ttatcaagac cctgcatcag caacaagtaa
    32101 ctttatttgc aacaggagga acttttgatt ttatacaatc actgaatatc cctgttactg
    32161 ctgttgaaga tgtgactgat tatccatcca tcttcggtgg gagggtaaaa acattacacc
    32221 ctaaaatttt tggtggaata ttaaatcgca gaaacgatgc tcatgacgtg atggaagttg
    32281 aaaaatacaa catcccttgt attgatttgg ttgttgtgga tttgtatccc tttgaagcca
    32341 cagttgcttc aggtgcagaa gcatctgata ttattgagaa aattgacatt ggtggtgtgt
    32401 cattgattcg tgcagcagca aaaaatttca atgatgttgc tatcatagct tctgcaaatg
    32461 atgtaacaga atttttgcaa acatacaatc agggaaatag tgaagttgat ttgtcattca
    32521 gaaaaaaact tgctcaaaaa gcatttgcaa taaccagtca ttacgattct gccattttta
    32581 attatttcta cagcaagaat gaaccatcag agccggtttt aaatttacaa ttcaacaatg
    32641 cagttacctt acgctatggc gaaaatccac atcagcgtgg tttttatttc ggtaacctga
    32701 aggaaacatt tgaacaatta ggaggaaaag aactttccta taacaacatg cttgattgtg
    32761 atgcagcaat taatctcatt gctgaattca gcaatgaaaa aggtgttact gcagcaatca
    32821 tcaaacacaa caatgcctgc ggaattgcta caagagaaaa agcaaccgat gcatggcagg
    32881 cagcattagc cggtgatcct gtttctgcat ttggtggcat aattgttttt aatcagcata
    32941 ttgacgaatc acttgccaaa gaaatcaatg ctattttctt tgaagtaata attgcaccat
    33001 cttttgcaga agaatcgata aaagtttttt cagaaaagaa aaacagaatt atacttcaat
    33061 taaaaaatca gcgtgttgcc aagcaaagca tcaagtctgc tttgggcgga tatttaatgc
    33121 aggaaactga cagtgaaata ttaaacacag aagggtggaa taacattact ttgcgaaagc
    33181 caacaactgc cgagacagaa gatatgcttt ttgctgtgaa ggttatccgt aatttaaaaa
    33241 gcaatgctat taccttggtt aaaaacaagc agttgattgg aagcggtgca ggacaaacat
    33301 ctcgtgttga tgcattgaag caagccattg caaaagctgt aaattttgga tttaatctga
    33361 aaaacagtgt aatggcaagt gatgcattct ttccttttcc ggattgtgta gagatagcta
    33421 aaacagctgg tattgaggcc gttgtacagc cgggtggtag tattaaagat aaagattcta
    33481 ttgaatattg taacaaaaat aacatgacta tggtattaac aggtaaaagg catttcaagc
    33541 attgagtctg ttggcagaca aagggttaaa aatgtctgca acaacagaaa aaaatgaaaa
    33601 aaaagcctac gcctgaagtt ctcatagtcg aagaaactat aaaggaaaac actaacaata
    33661 aaagaaaaat gggactattt gattttctga cccaggagat agccatagac cttggcacgg
    33721 ctaacacact catcatccat aatgataaag ttgtggttga tgagccaagt atagttgcta
    33781 ttgaccgtat gacaggtaaa gtacttgctg ttggcaagca ggctatgatg atgcatggta
    33841 aaacgcatga gaatattaaa accatccgtc cattaaaaga tggtgtaatt gccgattttg
    33901 atgctgcaga aaatatgatt cgcggaatga ttaaaatgat caacccggga agaagatttt
    33961 ttcagccatc acttaaaatg gttatctgta ttccatcagg catcacagaa gtagagaaac
    34021 gtgcagttcg tgacagtgca gaacatgccg gggcaaaaga agtttatctc attcatgaac
    34081 caatggcagc agctataggt atcggcattg atgtagaaga gcccatggga aatatgatta
    34141 ttgacattgg tggtggcacc agtgaaattg cagttattgc tcttggtgga attgtttgcg
    34201 accaatctat tcgcgttgcc ggtgatggat ttacgatgga tatatgggat tatatgcgca
    34261 gacaacacaa catccttatt ggagagcgaa gtgctgaaat gataaaaatt gaagtaggtt
    34321 cagcactgcc tgaattagac aaccctccac acgactacgc agttcatggt cgtgacctga
    34381 tgaccggtat tccaaaagaa attaccgtta cttattcaga aattgcacat gcacttgata
    34441 aaagtatcag taaaatagaa gaagctattt taaaagcact tgaaatgaca cctccggaat
    34501 tatcagcaga tatttaccga accggtattt acctaaccgg tggtggtgct ttactacgag
    34561 gattggataa acgtattggc atgaaaacaa aacttcccgt tcacgttgcc gaagatccac
    34621 ttcgtgcagt tgttcgtggt acaggtattg cacttaaaaa tattgacaga tataagtttc
    34681 tgattccata aacacattgc atactcatct aaagaggtta gctttataaa aagctaacct
    34741 cttttttatt tgctcagaac tttacttttt tcctcaataa aacttttcgt ttgaaatttt
    34801 taaagccatt ttttacatac tgactttaaa aacaaaatct aaaaataact acgtattcag
    34861 tataaagaaa ctgaaaagta atcttcaaaa aaaacaattc aacaaatgaa atgaacaaga
    34921 aaggtttatc caacttctaa gttgcttaaa ctttcaataa tcattttctt ttatgacaca
    34981 agtcatattt aaaagagctt tcctgaaata atcttgtcat cgaaaagtaa aattccgatt
    35041 aataaataat taaaaaccaa aaattatatg gaagaacaag agaataaaaa aaaggcttcc
    35101 ggacttcagg tagtcatgga tgatgttatg ctgcttcttt ttcttggagt aactatttac
    35161 gcaatttttt acatcatctg gggaataatg gagattgcca cagtacctaa tattcctgaa
    35221 tcagttaaac aatctatact taagtaaaat ctctaaaaca atctatttat gacattaata
    35281 gcacctaccg aagggtggtt taaaaagaag gtttcaaaag acgagcgaat gtggataatc
    35341 atatcgtttg tagtttgtat ttttcttttt gcatggatgg tactatggca cgtattcggt
    35401 cgccagaacc catcaaacac cacgtataaa actaacccta ttgaatttgc ccaactacat
    35461 gatgagtatg ttaaaagaaa catggtgggc atggacaatg ggatcccaat tgtaaaaccg
    35521 gagcccggca gcgatgtcat tcttatggca caaatgtggc gctggtcacc tgtacttatt
    35581 ttaaagaaag atgaatggta taacatccac atttcttcaa aagatttggt acacggtttt
    35641 tcattacaac cttcaaacat gaactttcag gtatatcccg actatgatta tgttttgaaa
    35701 tttaaaccca ctgttgccgg tgaagccaaa gtactgtgta atgaattctg cggtattggc
    35761 caccacacca tgattggaaa aattcttgtc attgagaatg atgctgatct ggcaaaattc
    35821 ggttacaaca atctgagtgc gccacctgca cgcacaggca aaggaactgc cgaagaagac
    35881 atttccggtt ggagtgaaga acaacttatt aaaggtggtg aaatggttta taatctgaaa
    35941 ggctgtatgg cttgtcacaa aacagatggc acctccgaaa taggtcctgc cttcaacaac
    36001 atttatggca gtaaggttaa tgtaaaagaa gatggcaatg ccaaagaaat ggttgttgat
    36061 gatgactata tcaccatgtc tatcagagag ccgcaaaaac aaattgttgt aggatttgaa
    36121 aaaacaccga tggctcagat gaaccttacg gacaatgaaa ttaagcaagt aatctcctac
    36181 attaaatcac ttaaataaaa tctaaagctt atgttcagaa tatgcgatat aaccggattt
    36241 aaagtagatc tgcaatcgga aaaatttatc agactaaatg ctgtcatggc agtagtttct
    36301 ttgctcttag caattacagc tgctatcttg cttgtgttta cacgctacca acctgtgcac
    36361 cttttagatg ctgagtggta ctacagacta acaacttttc atggattgaa tgctcttatt
    36421 tattggattg tatttttcga aattgcaggt ttgtatttcg gatcaacagt agtattaaat
    36481 cagaaattct gctctccaag attaggatgg ttaacattca tactaatggt tggtggcatg
    36541 gtcctttcca acgtaatggt attgatggga aaagcagatg tcatgttgac ctcttatgtt
    36601 ccactaaaag ctcatccact atattacctt ggtataattc tttttgctgt aggtgcttta
    36661 atgggtgttg gtttgttttt tgccaatatt gttaatgcca aaagagatgg aaatgttggt
    36721 gaaactttac ccctatttac ttatggttta atgacagctg caattcttgc tgttattacc
    36781 ttagcatcgg gcgctatcat ttacattcct actttccttt ggtctttagg actcattgac
    36841 tccgtagatc ctgccgttta caagttaatt tggtggggac ttggtcactc ttcgcagcag
    36901 ataaatgtgg ctgccatggt cagcatctgg tatttggtta ccttccttgc acttggcgga
    36961 acatcaatta atgaaagagt ttcaagaaca gcattcttcc tctacattct ctttatcagt
    37021 cttgcctctg cgcatcactt gttaaccgat cctgcaacat catcagcatg gaaagtatgg
    37081 aacacttctt atgcaatgta ccttgctgta ttagcatcaa tgattcatgc ctttgccgtt
    37141 cccagttcca ttgaatctgc acaacgcaga tggggattca acaaaggatt atttgagtgg
    37201 ctctctaaag caccatggtc aaaccccgct tttgcagcct gtgttctggc ttgtattggt
    37261 tttggtttta ttggcggtac taccggtgta attttcggaa tggaacagac aaacattatt
    37321 gttcacaaca ccattgctat tcccggtcac tttaaaggaa ctgtagttat tggtactaca
    37381 ctcacattca tgggcatcac ctactacctg attccactca tcttcagaag gaaaattgta
    37441 gcattcaaac tcgcacagct acaaccatgg atgttcttct ttggtgtagc catcctctct
    37501 gtatcaatga ttgcacttgg tcaattaggt gttccacgca gacattggga ttcaacattc
    37561 agcggaggtc ctttcactca tgagtttaat ccatctgtag atttcttctg gacattgatg
    37621 atgcttggcg gtactttagc atttctggct gcactcatct gggttctgca ggttgttgtt
    37681 tctgttttct tcggacccaa agtaaacgga cctgaagaca tgcagctgcc actttcacca
    37741 ttggctcctg ctgctaaaga acataaaggt tatgaggctc ccgggacttt aattcttaca
    37801 ctgttattcc ttgccacttt tgcagtactc ttctttgtta attggaaatg gcttgctgca
    37861 acatggtggg tgaaataact ttcaatactt taattattgg ttcgtgatta aaattaaaat
    37921 tcaacttaca ggcttagtaa ttttactaag cctgtttttt tccttaacat cattcgatat
    37981 tcctgatgag aaaaaattca ttggacggga ggtaaagaat attactattc ttgattctaa
    38041 gggaaataac gtgcaacttt tcgataatcc cacgggtaag cctgttgtat tgtcaccggt
    38101 ttatacgcgt tgcccaatgg cctgttcact tatcagcaat ggattaaaaa cctccattga
    38161 aaaattaggt actttaggaa aagactttac agtcatttca ttttcgtttg actcaaccga
    38221 cacccaagaa gatttacaag gatttgaagc gcgctggaaa atggatggtg taaactggaa
    38281 aactgttacc tccaacgcag caggcattga tgcactatta aagtcaatag attatcaata
    38341 tgaatatgat gagaaaagta aggaatatct gcatccaaac ttagttgtgg tgattacacc
    38401 cagtgggcga atttcaagat acatctatgg cgttgaaccc aaaacaattg accttgaact
    38461 tgcaatcatg gaagcacaag ttgaaaaaac ctcattcaac aattttttta aaacacttta
    38521 cattcgctgt tttacattta accctgcaac aaaatcttat cacctcgact ggaagtttat
    38581 ttctcaaact tctgccggaa tgatgttcat cattattgta ggttattttc tcattaaaag
    38641 tttatattcc aaaaacactg aagacagtgc agaattatga ttaataataa atcaagccat
    38701 tggctttttc aacgatttgc cggcattttc agccgtattt attcctcaaa gttaaatccg
    38761 ctttaccacc ttggcgacat tgctatactt atgtttattg tagcatgtat cagtggggtt
    38821 tatctttttt tgttttataa tattgatcct gcacatgcct acgaatcagt agaaaaaatt
    38881 tcggccaatc cgtttaatgc tgttatcaga agtattcatc gctacagcag cgacttgttg
    38941 gttattttta tcgtactcca ctttcttcaa acattactga ccggcaaatt caaacgtatt
    39001 ctttcatggg taagtggaat tatttctttg atggtagtgc tcatcattgg tgtaacaggc
    39061 tttattcttg tctgggacga aaaaggaaaa ttagtcggtt accttactgc aaaatttttc
    39121 tcagcgattc cactattcga cccttcaata gccggttcat ttatttcaaa caaccttgac
    39181 gttattggtg gcttctttaa agtttctgtt ttcggacata ttttcttttc cattttctta
    39241 gtcattgtat tatggctgca tgtaatgcgc attgccaaac caaaattatt ttcaccacgc
    39301 atcatctgga tttacagcat gattgcattg gttttagttt gtttggtttt tccggttaag
    39361 agcgaccctt cagcacaaga gcgatttata ccttacaata caactttcga ttggtactat
    39421 ttcttcggtt actcttttat gaaagttgtt tctgttccta tgaactgggt aattatgctc
    39481 gtatccggaa ccggtttagc attggttcca cttttattta aaaagaaaaa taaacctgcc
    39541 acaattgatt tagataaatg tgatgcttgt aacatttgtt cagaagattg cccttatggt
    39601 gctattgata tgctcattta taaaggtgaa cgcaaggcaa ttttagatcc tgataaatgt
    39661 atcagttgcg gaatatgtct tgcttcctgt aaagagcatg ccattacaat gcccggcatt
    39721 cctgaacaca atgtgttacc gcttaatctt gcacagaaaa gccaactgaa tgttgtttcc
    39781 tgctcgcagt ttgacgatgt aaaaattcct gatggaatta cccataaaaa gaaatccttg
    39841 ccttgtcttg gagatttaca catgcatgaa gttgagcaaa tgcttaacga agaatcggaa
    39901 ggtgttttat tattaggctg cgaagactgc tactatcgtt atggaagaaa tctggctatt
    39961 gcaagaatca acagaaaacg tccaccgggt ttagctaaaa aattatcacg cgacagaatt
    40021 caactcatca ctacaaatca ctgttctgaa aaagctataa gagcatttac agactcactt
    40081 gcatctgcaa agcctaatga tggaaaagca acaataaagg tattggatta ttttaaaacc
    40141 aatcatattg cagcaacatt gattctttgt gctttctttc taataatgcc ctttttcagc
    40201 aataccaaaa tttcattctt tgacagagat gaaaaactgt tggttctgaa cttcaaatat
    40261 atttcatcgc caacagaatt tcagggagta aacacaaatc agcaaagaca aatgcagtcg
    40321 ctaaagccca tagtaaaaag gcgaagccct attgttgttg aagtagaagg caacaaggga
    40381 aatgttcttc tcaaaaaaga attttctcct cgtggtttaa gaaaagacat ttctattttt
    40441 gtttatagtg aaattccaac aacagactcg ttagtcaata taaaagttac tgaaactgca
    40501 tttcctgaca aagtagttag tctgcacaat gttgccttat caactattga tggaacaata
    40561 atttcttttg ctgacggcaa attgaagcaa cttaaataag cacatcatca aattgctttt
    40621 tcattggaag aagtatttaa attacttttt tactaattca gaaaattctt tcttctaaag
    40681 ttgtattact tttactccaa atattttaat tatgtggaga attttaacaa ctattttttt
    40741 tgttagtctg ttggccaaca gtctttcagc atcaaaactt cagccgggtt ataatcctaa
    40801 agaatacatg gagttgctga gttttcaact cagtcaattt cataagcagg attctcttta
    40861 tcctgactca atgcattatc acaactttaa agaggtgtat aaatcacctg aaatggggtt
    40921 actgaatcaa tggtctttgt ggattggggc tgattctgta gcagtaatca gcattagagg
    40981 aacaatagca aacagtacaa gctggcttgc caatttttat gcaggtatga tacctgcaaa
    41041 agggcagctg attttagctc ccggtgatac ttttaattat cattttgctg atgattcaaa
    41101 agcggcagta catacaggtt ggacattggc tacagcatac ctcatgcgtg atatgcttcc
    41161 tcacattgac tcatgttata aaaaaggcat tacaaatttt attgtcagtg gacacagtca
    41221 gggtggtgcc atagcatttt tagtcacagc ctgtctttat gattatcaaa agaacaataa
    41281 gattgcttca gacttaagat ttaaaaccta ttgcagcgca gcaccaaaac ccggaaattt
    41341 atattttgct tatgaatatg aaaacatgac ccgcaacgga tgggctttta atgttgttaa
    41401 ttctgccgat tgggttcctg aaacaccaat aaccattcaa actaccaagg atattaataa
    41461 gaccaatccc ttggtaaatg caaaagcagg tatcagaaaa caaaaatttc cggccaactt
    41521 atttgtcatg cacatattta accgactgca aaaaacaccc tataaagcca tgaggcaata
    41581 cagaaaatat ttaggtggct ttgcaggaaa acttgttcgg aaaaacttag taacttttca
    41641 atcaccggat tatcttaaca gcagcgatta tgttcgaaca ggaacaacta ttgtgctcct
    41701 tgccgacaaa aaatattatg atttatttcc cgatagcaaa acaaaaatat ttgttcatca
    41761 cttttatgaa ccttatttgt atctggcggc taaaaggttg taatgttttt gattaaataa
    41821 cattataaaa aaaagcgagg ggagttaacc tccccccgct ttttacttcc tacaacttct
    41881 gagaagttgt gagttttaaa ttattcaacc actactctaa cctgattgtt gctgccttca
    41941 gtttgtacat tcagcatgta cgctccttta gcta
//
LOCUS       DAOQNP010000016        41816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_280_length_41816_cov_9.294988, whole genome shotgun sequence.
ACCESSION   DAOQNP010000016 DAOQNP010000000
VERSION     DAOQNP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 41816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41816
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_280_length_41816_cov_9.294988"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="PKY09_03850"
     CDS             <1..1397
                     /locus_tag="PKY09_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNB12019.1"
                     /translation="KGDVHDIGKNIVGVVLACNNYEIVDLGVMVAADKILQAARDHKA
                     DVIGVSGLITPSLDEMVHVAKEMEREKFTMPLLIGGATTSKVHTAVKIEPNYSGVVVH
                     VNDASRSVPVVSSLLSAEQKDMFIEEVKAENERTRSYHKAERSKGKFLSLKEAQQNKY
                     ELNGAVVTEPKFKGVKVFSDYDLSLLTEYIDWTPFFIAWEMKGSYPKILSDPQRGQEA
                     QKLFDDAQTMLQRIVKEKWLTANAVFGIFPAANKGDDIEVFDELRENKIATLHTIRQQ
                     SERATGQKNIALSDFIHPSDYIGAFAVSTGFGIEKWLKKFADDHDDYSAIMLKVLADR
                     LAEAFAEHLHERVRKEFWCYTTDENLSRQDLIKEKYQGIRPAAGYPAQPDHTEKYTLW
                     KLLDVEKNTGITLTEHLAMFPTAAVSGLYFAHPESHYFAVGKIQRDQVEDYAKRKSMT
                     VEEAEQWLGSILGY"
     gene            1489..2667
                     /locus_tag="PKY09_03855"
     CDS             1489..2667
                     /locus_tag="PKY09_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB12020.1"
                     /translation="MSKDRFQAPDYYLMDELLTDEHKLIRDSVREWVKKEVSPIIEDA
                     CQKCEFPKHLIKGLAEVGAFGPYIPTEYGGGGLDQISYGLIMQELERGDSGIRSTASV
                     QSSLVMYPIYTYGSEAQRKKYLPKLASGEWMGCFGLTEPDHGSNPGGMVTNIKDKGDH
                     YLLNGAKMWISNAPFAQVAVVWAKDEEGVIRGLIVERGMKGFTTPETHGKWSLRASAT
                     GELVFDNVEVPKENLLPNVKGLKGPLGCLNSARYGIAWGALGAAMDCYDSALRYAKER
                     KQFGRPIGGFQLQQKKLAEMLTEITKGQLLVWRLGKLKNENRANPAQISMAKRNNVHT
                     ALWCAREARQIHGGMGITGEYPIMRHMMNLESVITYEGTHDIHLLITGMDITGENAFT
                     "
     gene            complement(2682..3410)
                     /locus_tag="PKY09_03860"
     CDS             complement(2682..3410)
                     /locus_tag="PKY09_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HNB12021.1"
                     /translation="MKRFLFILLTLLSSTALAQQTGSGFAVVELFTSQGCGSCPAAEK
                     VLKKEMDDAVKNNKPVYFLSFHVDYWNKYGWKDPYSSIKYSRRQNNYVSATGSNEVFT
                     PQVFVNGNTGFVGSDSKRLNSEIEKGLKAVSKQKLTITVEKTLNDTLHLNYTAGKADI
                     NANIIVAVVQKSVTTKITKGENIGRTLVQDNVVRLYEVYPVATKGTLLLKTNKLELNK
                     NFQLIAFIQNKQSKKISAATALTL"
     gene            complement(3499..4095)
                     /locus_tag="PKY09_03865"
     CDS             complement(3499..4095)
                     /locus_tag="PKY09_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNB12022.1"
                     /translation="MEYIKHLSKDKKLAPIIKQHGTVELKKRKDLFLSLCGSIISQQL
                     SVKVADVIKQRFYDLFDGKPTPEKVLEATTESLRSLGLSNSKASYIKNIAAFAQENSL
                     QLNKVNKMSNDEFIEYITQIKGVGRWTAEMIMMFTLGREDVFSVDDYGIQQSVIGLYK
                     IKIADKKKLRLRILSISEQWAPYRTYACMYLWRWRDNA"
     gene            4315..4950
                     /locus_tag="PKY09_03870"
     CDS             4315..4950
                     /locus_tag="PKY09_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB12023.1"
                     /translation="MQIKKFTFNPFEENTYIVYDETSQCAIIDPGCYMPEEKEELLGF
                     IAAQKLNPVLLLNTHCHVDHIFGNKLIYDTFGLKPQFHQLELRLLEAAGEYSKQWGIF
                     LEPSPAAEKYLNEGDVISFGNVSLEILFTPGHSPGSLCFYHKQQKIIIGGDVLFQMSI
                     GRYDLPGGDEQTLLNSIRAKLFTLPADVKVLPGHGPETTIGFEKMYNPFLV"
     gene            complement(5040..5678)
                     /locus_tag="PKY09_03875"
     CDS             complement(5040..5678)
                     /locus_tag="PKY09_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12024.1"
                     /translation="MKKITFLLLLIAGNMFAQSNSKFEKTMVKQIDLLDKAKAGTDFV
                     NAANAFERIANAETKEWLPLYYQSYALVLSANDEKDNVKKDAVLDKALEIANKADKLS
                     PDNSEIVTLQSWITSMKIGIDPATRGQKLGMEAGMLTAHAIELNPENPRPYFLQGTGL
                     LFTPEQFGGGKAKALPILEKAIEKFNAFKPANAMMPNWGKEFAQKMLDECKK"
     gene            complement(5706..7862)
                     /locus_tag="PKY09_03880"
     CDS             complement(5706..7862)
                     /locus_tag="PKY09_03880"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12025.1"
                     /translation="MYLSKHVISWLFFLIIIQSVTAQTKISGTVTDQKNNPVAGANIV
                     IRDSYDGASSDASGKFQFTSDETGDVIIVCSLIGFSPSDKKIKLQGDAIEINFKLKET
                     SNELNTVTITAGAFEASDEKKMVILRPLDIVTTAGSQGDIYGALQTLPGTQPTQDQEG
                     LFVRGGDAAEAKTLIDGIEVANPYFSSVPDVPQRSRFSPFMFKGTNFSTGGYSAQYGQ
                     AMSSVLALESEDLPPRSIGNIGIMSVGGGGGYMHRWKKTSAGVFANYFNLAPYFAIIK
                     QNRDWDSPPQTFSSSAIFRHKTSETGIIKAFINYQYSDLSLNFENLEDVTGKSKNHFG
                     LNNYNIFSTLSYKEVLNKWTVQAAGSYSKDINRIYARPQHLLFSNQLWQGRAVVSRSI
                     SELSSIRGGAEVKYNDFSNLYNNFRVDSTEMFTAVFLEGDAYITRKLVARAGIRVENS
                     YLLRASNLAPRISVAYKLGKFDQLNVAYGDYYQTPDKNYLIFTNLDHFEKATHYILNY
                     QYVNEKRTFRVEAYYKKYNHLVQTTNMASNVYATAFATGDDGKGFAQGIDFFWRDKQT
                     FKYVDYWVSYSFLDTKRKYLYYPFEVQPTFASKHVVSVVMKRWFPKLRSSIGFTYVYA
                     AGRPYYNPNKTNENNFLSDRTNDYNNLSLTWSYLTSIGRHFTVISAAVNNVLGIESAY
                     SYRYSTDGLRRSAVGATSKRSFFIGVFISFGEDRTE"
     gene            complement(7917..8501)
                     /locus_tag="PKY09_03885"
     CDS             complement(7917..8501)
                     /locus_tag="PKY09_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNB12026.1"
                     /translation="MLKVDDILVSDQVITEKFICDLSKCKGACCVEGESGAPLEEDEL
                     KALEKVYPKVKPYLTDEGIKAIEAQGFYLKDDDDEWVTPLMWPGGPCAYTVFENGIAL
                     CGIEKAYRDKKIKFYKPISCHLYPIRVLKTKSGDALNYHEWEVCSPACKLGKKEKVRV
                     YEFLKTALTRKYGEAWYNKLIKTAQEYLQTLTKK"
     gene            8577..9332
                     /locus_tag="PKY09_03890"
     CDS             8577..9332
                     /locus_tag="PKY09_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB12027.1"
                     /translation="MNPIITVKNLVKHYNSFQAVKGISFDVYEGEIFGLLGPNGAGKT
                     TTLEIMETLRTKTSGEIIIKGYSVGADDDEIKKIIGVQLQAAGYYPSLSLLELIDLFA
                     GLYSQDVNAMELLLKVGLTDKAKSKYKELSGGQKQRFSIATTLINKPAVIFLDEPTTG
                     LDPQARRNLWDLIRQIKNEGTTVVITTHYMDEAEELCSRVAIIENGKIIALDTPDQLI
                     DDLVAKGFERKKEVKKANLEDVFLNLTGQEWRE"
     gene            complement(9343..9960)
                     /locus_tag="PKY09_03895"
     CDS             complement(9343..9960)
                     /locus_tag="PKY09_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12028.1"
                     /translation="MLKSGLALCLLLLLSSGSISAKDNIFKEQRIGFGYYPSFHGSQN
                     FWLNYSVNKRSRKTIYAIRGMYNFRSEKAQEVSNYAIGPCVSVKLLSFFKERLEIRPT
                     LYIPYVNFKYEKSTYPGSLYATQYNGAALLPGLNLQTELTDRIGVRYGANAGIYYAKT
                     REPIFEQKNGVTTYDVYTDKNMYWIMLIDFSITIKITKNYLPHQR"
     gene            10059..10499
                     /gene="rnpA"
                     /locus_tag="PKY09_03900"
     CDS             10059..10499
                     /gene="rnpA"
                     /locus_tag="PKY09_03900"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNB12029.1"
                     /translation="MNEGTLRNTFSKAERLCKRNDFELIFSQGSAFNRFPFRVHWLKL
                     KENTPSFPVKIAISVPKRKIKSAVSRNRIKRLIRESYRLNKQILSRELKKDEHVIHIM
                     FVYNGNINATFTEIQSKIILILQRLKELNEQTAEQDSDSHSKVL"
     gene            10450..10671
                     /gene="yidD"
                     /locus_tag="PKY09_03905"
     CDS             10450..10671
                     /gene="yidD"
                     /locus_tag="PKY09_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNB12030.1"
                     /translation="MSKLLNRILIAIVKFYKMAISPLLPMSCRYYPTCSEFCMEALRK
                     YGPLKGGFLSLKRILSCNPFGGHGHHPVP"
     gene            10723..12399
                     /locus_tag="PKY09_03910"
     CDS             10723..12399
                     /locus_tag="PKY09_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB12031.1"
                     /translation="MRKRIRLYVAVILVGISAFSVYSFSDDYFEISRNLDIFATLFRE
                     LNIYYVDETNPGDLMKKGIDDMLASLDPYTNYIPESEIEDYRFMTTGQYGGIGSLIGQ
                     RNNEVIITDPYEGFPAQKADLRAGDVIVELDGKPIKGKKNEEVSKLLKGQPKTTVKIT
                     IRREGEPNLIDKNVVREEIKINSVSYSGIIDNDLGYIRLTGFTENAGQEVKNALQALE
                     AKTKLRGLIFDLRGNPGGLLNEAVNIVNIFVNKGVEVVSTKGKAKEWDKTYHALNNPV
                     DTEIPVAVLVNSSSASASEIVSGSLQDLDRGIIIGQRTFGKGLVQTTRPLSYGAQLKV
                     TTAKYYIPSGRCIQALDYAHRNDDGSVGKIPDSLITQFKTKAGRTVYDGGGVMPDFVT
                     DIRQLSPISQSLLLKYLLFDYATMYRNTHPSIAPAKEFHLSDAEYAEFVKWISAKDYD
                     YETKSEKILDEFKTTAEKEKYFSAAAAEFEVLKKKISHDKNADLQKFKDEITELLENE
                     IVSRYYYQSGQIEASFKNDLEIKKAAEALKNKDVYSSIMKSSMASVREKH"
     gene            12415..13983
                     /locus_tag="PKY09_03915"
     CDS             12415..13983
                     /locus_tag="PKY09_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB12032.1"
                     /translation="MTGRLQSISKPLFFAGVLLFAVGLPLSMFLMSVSQLFIIVGWLL
                     DGDLKEKLKTAFNNKVVLLLVAFFLWHVVGLLYTTDFHYGFKDVRVKLPLLIIPIIFS
                     SMPVFSDKQFKTVALVFVAAVLVSTLISMCIYVGVIPIKFQNVRDISIFISHIRLSLL
                     ICIAVYLSFWLFRRIKFGYGLLLLVVWFVFFLTLIESVTGLGIMAVVLLIYAINAFLK
                     NKSVLRKGVIAFSVVVAVSLLAFNIKNTWHDVTFFNDAELRNLAANTANGTPYTHDTL
                     NREAENGHPVWINICEPELREAWNQRSRIMYDDRNLNGDEIKRTIFRFLASKGLAKDA
                     LAIKQLSDAEINAIEHGVANANDLTDSNIHNRLRATLWELNRYRTGGNPSGHSLTMRF
                     EFWMTALQIIKQQPLTGVGTGDVPDAFAKKYDETNSMLDIKFRLRSHNQFLAVAVALG
                     IPALILFIIMLFYPVFQSQYSRSFLFMTFWITAFLSMLTEDTLETQAGVSFFIFFSCL
                     FLFLKNKELDKNII"
     gene            14038..15225
                     /locus_tag="PKY09_03920"
     CDS             14038..15225
                     /locus_tag="PKY09_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12033.1"
                     /translation="MKNYIIVILFLFPFLAQSQTGEDSLHTNAANEKQTYISGYGEAM
                     VSYDSRYRTAQANFTRAVVFVGHKFSKNISFLSETELADARVEGGKAGGEIALEQAYL
                     RFQLNRKMHLQAGLFIPRIGIINENHLPTTYYGNHRPFTETFLIPSTWRELGVALYGS
                     SDAVTGLNYSIAVVNGLDASGFSNGTGIRGGRFEGGNATASNIALTGSVQYVMQHFTF
                     QISAYYGGSAGLTKREADSLQLNSGMFGTPVGLGEVNVQYRKDRLTAKVLFAAISIPE
                     ADKINRAYANNTAKLSIGGYAEVAYRILKKLKTDAFIRYENFDLNSSIPKNGIKNEIL
                     KQQYIIAGFSCFPTPGVVVKLDYVYKQTGDLNPALNLNPFPNAPAYYTTNSFINLGLG
                     YSF"
     gene            15228..15650
                     /locus_tag="PKY09_03925"
     CDS             15228..15650
                     /locus_tag="PKY09_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNB12034.1"
                     /translation="MKRRNFIKNTCMSCGMMAAGGSLLSLLSSCKSTSIFKTEAVNHL
                     LTVPLSAMNDKKILIVRNASAEYDILLVKKSDNLFTALEMKCTHRANPLNASDGGLYC
                     TLHGSRFDLDGNVVAEPATQPLKKYPVIVNNSLIQISI"
     gene            15658..16707
                     /locus_tag="PKY09_03930"
     CDS             15658..16707
                     /locus_tag="PKY09_03930"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNB12035.1"
                     /translation="MQYKFILLSLASVLVLSSCKKDKDSSDNYSSTQILNDFSVNVAQ
                     ATYNDLASKTAQLHQAITDFTQNQTQANLDYCKLLWKESRQAWEQSEGFLFGPVATDD
                     IDPRIDTWPVDFNSLDSVLNNQSVYDETYVNSLEDALKGFHPVEYLLWGVDGNKTILQ
                     FTSRQLDYLEALAANLKTLTQQVATSWTTGNYHNEFANAGNGSQVFTTQHAAFMEMVN
                     AMAGICEEVADGKMEDPLVSQNAMLEESPFAKNSIIDFTNNIKSVENVYLGKYTSQGQ
                     SLESFVRANNLSLDGSIKTKISAAVTALGNITVPFGQAIFTQQTQVINAQNAIRELKE
                     VLENDLANLVSQKIQ"
     gene            16710..18089
                     /locus_tag="PKY09_03935"
     CDS             16710..18089
                     /locus_tag="PKY09_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNB12036.1"
                     /translation="MKRNIVVVIIFLILFGAQSCRKAEVLSESEWNEWLSGGKQTLFD
                     ESAGAFSSVFPDISAHNAMIHEMGDKAFEATFVSAPAPLNPGLGPIFNNVSCASCHIA
                     DGRGKPPLSSSEPLSAMLIRVSIPGVASNGGPLAVPGFGVQLQQRSINGVAKEADVII
                     NYSEQTFSFPDGETYSLRTPTYQLANSYIPLPAGVMLSPRMAPPVFGLGLLEAVDESE
                     VLKYADENDVNGDGISGKPNYVWNALKGERTLGRFGWKANNPTVLQQTAGAYNEDMGI
                     TSYVFPEESSKGQSQYDNRNDDPEIPDSVLRAVSFYVQTLAVPVRRSADDVIVLQGKK
                     LFNDAKCSSCHRPAMQTGVRVDFPEVSNQRIFPYTDLLLHDMGDGLADHRPDFDANGN
                     EWRTAPLWGIGLTQKVNGHNFYLHDGRARSLQEAILWHGGEAESAKNTFKNMSKNDRQ
                     ALIKFLQSL"
     gene            18474..20609
                     /locus_tag="PKY09_03940"
     CDS             18474..20609
                     /locus_tag="PKY09_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNB12037.1"
                     /translation="MRHLSHIPVLRILIPFVAGIVVGIFMAVPAWLAIALLLSGFIGV
                     LAFHFSKYFSQHFKLRYLFGIVIHLFLFSSGVLLTQLKTKLYDPYHYTQFEDAEYFLL
                     RITEPYHIKQKSLKAEAEIEAVFENNKWRPCRGKTLLYFASDAAAKALQYADRIVTNA
                     KPQPIAPPQNPGSFNYKRFLGFHQIHHQFYLKSGRWATAGKGFSNILLSTAFELRKKF
                     LNEIQGQILVPQEVAVCSALLIGFNDLLDDDLLSAYASSGALHVLSVSGLHVGLIFVI
                     LQRLIFIPENKKYLNLFKQLLILLLIWFYAMLTGLSPSVLRSATMISIIIIAKQLGRD
                     NYMLNTTLASALLLLLINPFSITEVGFQLSYLAVIGIVYLHQRLVTVLYPTSMLMNQA
                     WQLTSVSLCAQLVTFPLGILYFNQFPNLFLVSNLLVIPISTGIIWVSVGATIISALPY
                     MSSLVFYSWKVTFALTWLMNQLLLFLENLPFALISGLYIDIADTWIIYGIIAFVVWFL
                     IYNYRYALYGALTLTIIFLITQLWHEWNILQQKNITVYALKNSTAIDIAQARKRLVIT
                     PKQIMEDKSLLRFNINRYWYETGKREMLHIPFYQQPTESKLKEIFNSKITGNVATVQD
                     GFIQIDSRLYFIPGNWLPRKVPLSKIKVDGIIITSRFKNKLPTLFKYFDSDLLIIDLS
                     SRAKVDAGKLPVSLQIWDCRKQGAAQVKI"
     gene            complement(20675..20917)
                     /locus_tag="PKY09_03945"
     CDS             complement(20675..20917)
                     /locus_tag="PKY09_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12038.1"
                     /translation="MAYKQIIIPERFLNYLYHAFKNISNPENHRVKLITRDGQKYRSL
                     QVINGCVLLIERSAPVTSNDIEILQLDMDFVPVKVK"
     gene            21040..22323
                     /gene="tyrS"
                     /locus_tag="PKY09_03950"
     CDS             21040..22323
                     /gene="tyrS"
                     /locus_tag="PKY09_03950"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNB12039.1"
                     /translation="MGFIDELRWRGMLQDIMPGTEELLNKETVTGYIGFDPTSDSLGV
                     GNLVQVTTLMHFQRAGHKPIALVGGATGMVGDPSGKSAERNMLSEEILQHNLNCQKKQ
                     LEKFLDFDCGKNSAEIVNNYDWFRDVKFLDFIRDVGKHITVNYMLAKDSVKNRLENGM
                     TFMEMAYQLVQGYDFYHLYYAKNCKLQMGGSDQWGNIVTGTELLRRKGRVEDAFALTT
                     PLIKKADGTKFGKTESGNLWLDRNKTSPYAFYQFWLNASDEDAKNYIRIFSLKNQLEI
                     EALESEHFAAPHLRLLQKSIANEITTRVHSQQDCERAIEISEVFFNGTDKELAQLTEA
                     DLQDVFNGLGKNAFEVELQKIQAGMQALDLFAAETSIFTSKGEARKMITANGVSLNKL
                     KFSDPSQIVSKEQLIANKFILIQKGKKNFFLIKAV"
     gene            complement(22360..23271)
                     /gene="gldA"
                     /locus_tag="PKY09_03955"
     CDS             complement(22360..23271)
                     /gene="gldA"
                     /locus_tag="PKY09_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNB12040.1"
                     /translation="MSVSVKNVTKIYGKQRALDNVSFEINTGQVVGLLGPNGAGKSTL
                     MKIISCFIPQNEGKVSVCGFDTLSQSLEVRRNVGYLPEHNPLYPDMFVKEYLQFVGGI
                     YLSAKEAAKRADTMITLTGLTPEYKKKIGALSKGYRQRVGIAQALIHDPAVLILDEPT
                     SGLDPNQLVEIRALIKSIGKHKTVILSTHIMQEVEAICNRVIIINKGKIVADDDTGML
                     RQKAKPVHEITVEFDGKIDAKWLQDIPTVSKVIHHSGNTWKVNYSGDDDVRKAVFTMA
                     VSHNIAVLSMQKEESRLEDVFQLLTQG"
     gene            complement(23287..24165)
                     /locus_tag="PKY09_03960"
     CDS             complement(23287..24165)
                     /locus_tag="PKY09_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNB12041.1"
                     /translation="MNQIPESELILNPDGSVYHLNLLPEDISDVVINVGDPDRVSKVS
                     SFFDSIEVKKQKREFVTHTGHYKGRRISVISTGIGTDNIDIVYNELDALVNIDLKTRT
                     VKPSLTALNLIRIGTSGALQPEIPVDSFVFSTFGLGLDGLLNFYQYTNTPEEQAIIES
                     FRKHYPNNCKLPESYLTRSSLKLEHALSEGMHKGITASCSGFYAPQGRVLRYQLARTD
                     MIEKLNSFRHGEHKVTNFEMETGAMYGLAKILGHHCCAVNLIVANRIAQQFSKKYEER
                     MHQLIQTVLDRISAQL"
     gene            24332..25219
                     /locus_tag="PKY09_03965"
     CDS             24332..25219
                     /locus_tag="PKY09_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB12042.1"
                     /translation="MNQEKKVIVTPIDFSEQSLIALSQTFNLARLTGSHIRLLHVIDQ
                     DFVSHLTNSIFSKENYADQIRNDIERRLDELASKVHKEEKISTSTHIRTGKIYNEIVA
                     EANDVDAAFIVMGTQGASTLKKKFLGSNTSRVIKEALCPVITIKGHEHLKGCKDIALP
                     LDLTKETREKVNYAIDMAKLFGAVVHIFSVLETDDEFLVNKLERQLQQVKQFITESGV
                     ECQGELVPQSDSNVSLSIIEYARIKNCDLIIIMTQGETNIADLFIGTAAQEVINNSEI
                     PVLCIRPMVRKDTTQYVLS"
     gene            25231..26118
                     /locus_tag="PKY09_03970"
     CDS             25231..26118
                     /locus_tag="PKY09_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB12043.1"
                     /translation="MSSNQLFEIKRILIPYDFSETAALSLEHAVFMAKLLKADIYLLH
                     ILESVSFTSAFGAAFGNVDKKLESESNTKLDEIGKQIHMNNGITVKSITEVGRIYRKI
                     VDVAKRENIDIIIMGTHGVSGYKKFNVGTNTSKVVEEAGCPVISVQTHSKKIGFKKIV
                     LPIDNSPQSRQKVSYALAVARSYGSHICIAGLINFGNEDNKRMFKLKVDQVEEWLLQH
                     GVSVEKKFVEGDNLAKMTMQAAEEWDADLIVIMTEQEPSITGFFLGTYATQVVNHSKI
                     PVMAVHPSESDGSTITVGY"
     gene            26208..27890
                     /locus_tag="PKY09_03975"
     CDS             26208..27890
                     /locus_tag="PKY09_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNB12044.1"
                     /translation="MPSFKGSLRFIFKTLLWTAGILIILFLLFLWRIQIPDPAISSKL
                     KPGDLKRIQTGVDSYRIGNNWLRKNQQGVWEMYIEGNDYERGVVYGVLAKELIEKQEE
                     YFVDQINEIIPNKFFLQFIKMFVAWFNRDIYKYIPAENLREIYGISLSFSDKYDYIGP
                     KYYRILNYHAAHDIGHALTDFQLVGCTSFAANDSLTDDHKLLIGRNFDFYMGDDFARN
                     KLLLFVKPDSGYSFVSYSWAGLTGVVSGMNEKGLTVTINAAKSDIPTTAKDPISLLAR
                     EILQHATNIKQAIAIAEKRETFVSESLLIGSAQDSTAALIEKTPTRMDVYHSPHELLV
                     CSNHYQGAELKNDPHNISNIELSDSKFRFDRITELLHENYPLSPTKAAAVLRNKTGMG
                     NKEIGYGNPKAIDQLIAHHSILFKPQSSDFWVSTMPYQEGMYLHYNLADVFKGNIRNT
                     DSLNIPADDFVNSADLKKFEAFKEVKQRIYRHNTFGVSLKLSSAEIKEYIKNNPMSYV
                     TYMSLGDYFYHEKNYTMAEVYYQEALTKEIASKAEEEKIKQAITTCQQKAAK"
     gene            27887..29173
                     /locus_tag="PKY09_03980"
     CDS             27887..29173
                     /locus_tag="PKY09_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB12045.1"
                     /translation="MTLLGNIEFQTTEEIRSMQQQRLADLLKYVSAHSNYYQNLFQQY
                     HINPDKISLDNLHELPFTTKDNLACSNDDFLCVDKNQIADFVTTSGTMSDPVTFYLTS
                     ADLDRLAYNEAISLQCADGSSNDIFQLMTTIDKLFMAGIAYYLGIRKLGAGSIRVGPG
                     TPHAQWEYIHRFKPTTLIAIPSFIPQLLQFAKEKQINFQSTSVKKIVCLGEPVRNADF
                     SLNELGRRITENWDVKLYSTYASTEMGSAFTECGYGMGGHSHPELLILEVVDDNGERV
                     RDGELGEVVVTTLAVEGMPLIRYRTGDLCHVYYNKCLCGRATTRLGPVMGRKQQMIKF
                     KGTTLFPQTIFNVLHKMPLIELYQIEVARDEFNNDSVTVLLNSALKSDEFEHNLLSLF
                     KSILRVTPHIAFITEQELHSRVYKTDKRKPEKIVYV"
     gene            29309..29605
                     /locus_tag="PKY09_03985"
     CDS             29309..29605
                     /locus_tag="PKY09_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12046.1"
                     /translation="MICFLELIPFLKKGVIVHIHDIYLPYDYPQFMCDRFYNEQYMLA
                     AFILANPIKYKTILPCYFISEDIELAKTISPVWSHPNLTHVEKHGGSYWLMINE"
     gene            29648..30277
                     /locus_tag="PKY09_03990"
     CDS             29648..30277
                     /locus_tag="PKY09_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12047.1"
                     /translation="MKKSLLTLMLSALMLGAFAQGKMNELFKNNVKLIFMGYDLSQAR
                     FIGTDGFTDPAAIKDKFVAAWNNTFVAEYEKFSLQKAFKLTNDKYETNVDVVQDINGK
                     LNIHERVMNGSYSIGEDDVKKAVSHYNSKEKDAVGLVYVVESFNKTLEKAVIWVTFVD
                     LSNNKMLYTEKIEEKAGGFGLKNYWIAPATKLKKDIEGKYYKTWKNKFK"
     gene            30282..31007
                     /locus_tag="PKY09_03995"
     CDS             30282..31007
                     /locus_tag="PKY09_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNB12048.1"
                     /translation="MNKNSCFHLSRQIILFCLLSFLLSSCRGLFLSKEKAYSKAIATK
                     PFDAIIVPGFPYFEGQPWPQVVQMRMIWAKFLYQNGYTKNIIFSGNAVYSPFVEGKTM
                     KAFAIAMGIPEEHIYVEDKAEHSVENVYYSYRLAKKLGFKKIALATDPVQTRSMVSFI
                     HRFSLPISYLPIMYKTLRLLPHNEPDIEGKLQRVENFISLPEKETFKQRFAGTLGKNI
                     KWHIEDLKKKRLRKKYSHNIVQE"
     gene            31012..32100
                     /locus_tag="PKY09_04000"
     CDS             31012..32100
                     /locus_tag="PKY09_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017045151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNB12049.1"
                     /translation="MSSAENTMTEKRELSAFEAKNEAQKLAFAPVVFQVVVAMQRLEI
                     LDLICKNRKGISLESISDKTGVSLYGVKVLLEMAANADIVKYIDDNTVTVTMLGYFLN
                     SDEMTRVNINFTHDVCYDGFKFLTESIQNGKPEGLKTLGNWPTIYPGLSSLPERVKKS
                     WFEFDHFYSDEAFRDALNIVFQEKPSTIFDIGGNTGKWAFASCEYNPDVKVKILDLPQ
                     QIAVAKANAEKRNLTDRISYFEINLLDKSQKIPKGADVVWMSQFLDCFGEDEIVAILE
                     NVRQAAAPHTTVFILEPFIDNQKFDAAAYCLTATSLYFTALANGNSKMYSVKAMTGLV
                     ERAGLEVVETFPLIGESFHTILKCKVKA"
     gene            complement(32142..33443)
                     /locus_tag="PKY09_04005"
     CDS             complement(32142..33443)
                     /locus_tag="PKY09_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12050.1"
                     /translation="MKSIKIMGVLLLAIGTVALLTSMKKGSDRYKVKTKGEKGCVFRS
                     VFEAEGIDSNFIYSTPVKVKESMTSALEWISKAQLKDGGWGAGSHARQSVFDPHAVSS
                     DPATTAMVAMALLRTGNTPTTGKYSANLSLATEYLLKQIEAADAQTLNITNQTATQPQ
                     VKLGQNIDVVLATQYLTNLMDYITNNQTLKTRVSKSLDKCVVKIQHAQSENGSIKGAG
                     WAGVLQSSFATNALESAKAAGASVDDEKLERYKNYQKGNYDAKTNSVATGDGAGVMLY
                     SISGTARSTAREASIAKDKIAKAKKEGKLSPTSTVTSESLQAAGMNEADAYKYAAAYE
                     INQAASRQAQRNDVMNGFGSNGGEEFLSYLQTGEGLIISKDKEWHKWYDNVSGRLLNI
                     QNNDGSWNGHHCITSPVFCTATSLLILSVNNDIEKLVSLNK"
     gene            33541..34050
                     /locus_tag="PKY09_04010"
     CDS             33541..34050
                     /locus_tag="PKY09_04010"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HNB12051.1"
                     /translation="MKHVNLFSVPVAILFLSAIFSCNTSKKVSENNSADSLLISLSRY
                     PCFGRCPYYDATLYKSGYAVINRKGFMDSLGIYATQIEKQELNSIIEEAKQAGLIAMP
                     DSFYNPGIADYPATIVTVNLNGKAKRIFDGVPESPTSLKDFEEKVHQLFTASGRQWKL
                     MQKPAQQQD"
     gene            34251..37562
                     /gene="secA"
                     /locus_tag="PKY09_04015"
     CDS             34251..37562
                     /gene="secA"
                     /locus_tag="PKY09_04015"
                     /inference="COORDINATES: protein motif:HMM:NF009536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNB12052.1"
                     /translation="MIDFINKTISKIFGSKAESDYKALSPVIDAVNAVAENIATLSND
                     ELRNKTNEFRTRIQDYLSEIDAELTQLNEEAEADSEMDANRKEEIYNRIDALKKERNK
                     KTEEVLNEIQPEAFALVREVAKRFTENAELAVEANDNDKNLVTKRDGIRIENGKAIYS
                     NTWTAAGSKVLWNMIHYDVQLIGGSVLHQGKIAEMATGEGKTLVATLPIYLNALAGAG
                     VHVVTVNDYLAKRDAEWNGPLFEFLGLTVDCIDKHQPNTEERRKAYLADITYGTNNEF
                     GFDYLRDNMTRHAHEMVQRPHHFAIVDEVDSVLVDDARTPLIISGPTPKGDSQEYFAL
                     KPKVEILFNAQRNYINTVIADAKKKLGENKPDEAGMPLIRAYRGLPKNRALVKFLSEQ
                     GVRALLQKTENHYMQDQSKEMHKVDAELFFVIDEKNNTVELTEKGIELITANNEDPHF
                     FVMPDVGASIAEIEKSNGTNEEKLERKEALMRDFSVKSERIHTINQLLKAYTLFEKDD
                     EYIIADGKVKIVDEQTGRVLEGRRYSDGLHQAIEAKEGVKIEAATQTYATVTLQNYFR
                     MYHKLAGMTGTAETEAGEFWQIYKLDVVVIPTNKKIIRDDREDLVYKTKREKYNAVIE
                     EIAKLTAAGRPVLVGTTSVEISELLSRMLKQRNIKHNVLNAKLHKKEADIVAEAGQAG
                     TVTIATNMAGRGTDIKLGQGVKEAGGLAIIGTERHDSRRVDRQLRGRSGRQGDPGSSQ
                     FFVSLEDDLMRLFGSERIAKLMDRMGIEEGEVIQHSMITKSIERAQKKVEENNFGIRK
                     RLLEYDDVMNAQREVIYKKRRHALFGERLALDIMNMQYDVCESIVSSYNQERDFDALQ
                     LDLIRYLSIEAPIDAKAFLQIKLEDLTQMVFEKANSHYRQKSNLIAEKAFPVIENIFT
                     NQGQTIVDVVVPFSDGTKAMNVIAGLKKAYDSNCKELISALEKSSVLNFIDEAWTEHL
                     REMDDLRQSVQNAVYEQKDPLLIYKFESFELFKQMIDRVNKEIVSFLIKSYIPVQNQE
                     NVREAKVQQQRTDMSKMQTSHSESDSGGQGRPQGPPPPPKQQPAKAEIKVGRNDPCPC
                     GSGKKFKNCHGAGVD"
     gene            37664..38686
                     /gene="pheS"
                     /locus_tag="PKY09_04020"
     CDS             37664..38686
                     /gene="pheS"
                     /locus_tag="PKY09_04020"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNB12053.1"
                     /translation="MSQELIEQHIQEAETFSIQSPADLENFRLKYLSKKGVLADLFAL
                     MKNIEPAERKNYGLQVNLLKQKTEAIFESFKEKFENVTAEEKKIDITLPGEPMSEGAL
                     HPLSIIRNRIIEIFSAVGFTVSDGPEIEDDWHNFTALNFPEEHPARDMQDTFFIRKNP
                     DLALRTHTSSVQVRMMEKHKPPLRTISPGRVYRNEAISARAHCFFHQVEGLYIDKDVS
                     FADLKQTLLYFAKEMFGADTEIRLRPSYFPFTEPSAEMDISCNFCKGEGCNICKYTGW
                     VEILGCGMIDPAVLDNCKIDSNVYSGFAFGMGIERITMLVYNINDLRIFSENDVRFLK
                     QFSGIG"
     gene            complement(38698..>41816)
                     /locus_tag="PKY09_04025"
     CDS             complement(38698..>41816)
                     /locus_tag="PKY09_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB12054.1"
                     /translation="IYDYYYYTDQLTFTSVHGCTATVAKQKNDTVFSITARFMPNKSI
                     GCAPMTVIFSDSSRSRETIVNYTYLFGDGTQTTGSNGVVSHTYNTPGIYYVRLVVTNS
                     AGCKDTSFVIPIYVGSAVTTAFTVVPTTICAYTPVQFNVLNPDTNNIYHFSGDGGMLS
                     HCYNQTNPVWNFNTQTGQQTITLSTSNNGCVSTATQTVTVKGPMAKFYVAGNCATPFK
                     YSFPATINDATTWNWDFGDGTALINSGNINPTHTYAATGDYWVKLTSFNTTSGCAPDI
                     DSNIVRVRNLSASFTGDSAICKGYTANVDASSSTDAYGSCNEGFLWYWGGNAFPHNSA
                     SPIASHTYLNNGVYFIKLVTTDINGCVDSVKRKIKVYSSNAYFKTNKNYGCLPLTINF
                     TDSSFADTTIASYSWDFGDGNTSSVKNPSHTYTTAPAGGSWTVTLIVTDTIGCTSVFQ
                     KTITASLPDTLFSSTKTNICVGEAIVFNPHFTGHSSYSWNFGDNTTSTQVSPSHAYNA
                     AGAYTVTLSVTDSIGCPGSKKITNYINVQAFPKAGFTSSADSMLNKCYPLLVNFYDTS
                     IVSVFGSRKWNLGNSSNIFTTPVAGTIYQTPGIYYIQLIEETTFGCRDTAYDTLRVLG
                     PVGNFNIAPSTICKGQSITFSIKDTADLGAFSWDFGDGMTSPGVSPITHTYNINPVSG
                     QTLVGLVMWSADSACTYTQTNPVFIRKVIANFNINSGDTALCVGEPVTLVNTSLNASN
                     NYWNFGNGQTFNGATPPSYTINQTGVYTITLAIDNSTWGCKDTIRKKISINNLPIVSA
                     TGGDTCLGKPITLHSSGGVSYLWSPAAGLSSTTIANPVATPGQSTVYTVLITDVNGCT
                     GVATAPVTIYSPPPQVNVTHLLFVGDSIQLNTGLTNNLIINWNPVNGLSCVNCPDPWA
                     QPLVNTLYTATFTDSAGCFSGISRFNIEIDPRITIDVPTAFSPNGDGDNDVIYVNGLG
                     LKRLIEFKIYNRWGQLIFESNDLNKGWDGYFKGQLQNVETYVYTAKAEGWLNGKIVSK
                     KGTFNLLR"
BASE COUNT        13178 a         7340 c         8348 g        12950 t
ORIGIN      
        1 taaaaggtga tgtgcatgat attggaaaaa atattgttgg tgttgtactt gcctgcaaca
       61 attatgaaat tgttgattta ggtgtaatgg ttgcagctga taaaattctg caggctgcac
      121 gcgatcataa agcagatgtc attggtgtca gtggattgat tactccttca cttgatgaga
      181 tggtgcatgt tgcgaaagaa atggagcgcg aaaaatttac aatgccatta ctgattggtg
      241 gagctaccac aagtaaagta catacagccg taaagattga accaaactat tcaggtgttg
      301 tagttcatgt taacgatgct tcgcgcagtg tgcctgttgt gagcagttta ctttcagcag
      361 aacagaaaga tatgtttatt gaagaggtta aggcagaaaa cgaaagaaca cgctcctatc
      421 ataaggcaga acgatctaaa ggaaaatttt tgtcgttgaa ggaagcacag caaaataaat
      481 atgagctgaa cggtgcggtt gtcacagagc caaagtttaa aggtgtgaaa gttttcagtg
      541 actacgattt gtcgttgttg acagagtata ttgactggac gccatttttt attgcctggg
      601 agatgaaagg cagctacccg aaaatattga gtgatcctca aagaggacag gaagcgcaaa
      661 aactttttga tgatgcacaa acaatgctgc aacgtattgt taaagaaaaa tggctgactg
      721 caaatgctgt attcggaatt tttcctgcag caaacaaggg tgatgatatt gaagtgtttg
      781 atgagctaag agaaaataaa attgcaacac ttcataccat tcgtcagcag tcagaaagag
      841 caacagggca gaagaatatt gcattgtctg attttattca tccttcagat tatattggag
      901 cctttgctgt atcaacaggc tttggaatag aaaaatggct aaagaaattt gcagacgatc
      961 atgatgatta ttcagccatc atgctaaaag tattagccga ccgactggca gaggcatttg
     1021 cagagcatct gcatgaacgg gtacgcaaag aattctggtg ttatactaca gatgagaatc
     1081 tgagcagaca agatttaatc aaagaaaaat atcagggcat tcgtcctgct gccggctatc
     1141 cggcacagcc cgaccataca gagaaatata cactttggaa attgcttgac gtagagaaaa
     1201 atacaggtat tacgcttaca gaacatttgg ctatgtttcc aactgctgct gtcagcggat
     1261 tgtattttgc acatcccgag tcgcactatt ttgcagtagg aaaaattcag cgtgatcagg
     1321 tggaggatta tgcaaagcgt aaatcaatga ctgttgaaga agctgaacaa tggctgggaa
     1381 gtattttagg ttattagttt gcggtggtta ttcttacaaa tttaattcct tccaagactg
     1441 attttccttc aaccaaaaaa gctatttttg cagactaaaa tttaaaatat gtcaaaagac
     1501 aggtttcagg caccggatta ttatttaatg gatgaattat taacggatga acataagttg
     1561 attcgcgaca gcgtgcgcga gtgggtaaag aaagaagtat caccaataat agaagatgct
     1621 tgtcagaaat gtgagttccc aaaacatctg ataaaaggat tggcagaagt aggagctttt
     1681 ggtccttata ttccaacaga atatggtggt ggaggattgg atcagatcag ctatggtttg
     1741 attatgcagg aattggagcg tggcgacagt ggcattcgct ctacagcatc ggtacaaagt
     1801 tcattggtga tgtatcctat ttatacttac ggtagcgaag cacaacgtaa aaaatattta
     1861 cccaaacttg cttcaggtga atggatgggg tgttttggtc ttacagagcc tgatcatggg
     1921 tcaaatcccg gaggcatggt gaccaacata aaagataaag gtgatcatta tttactcaat
     1981 ggtgctaaga tgtggattag caatgcaccg tttgcgcagg ttgcagtagt gtgggccaaa
     2041 gacgaggaag gtgttatcag aggtttaatt gtagagcgtg gcatgaaagg atttacaaca
     2101 cctgaaacac atggaaaatg gagtttacgt gcaagtgcaa cgggtgagtt ggtttttgat
     2161 aatgtagaag tacctaaaga aaatttattg cctaatgtaa aaggcttaaa aggaccatta
     2221 ggttgtttga acagtgcacg ttatggtatt gcatgggggg ccttgggtgc agcaatggat
     2281 tgttatgaca gtgctttgcg ttatgcaaaa gagcgtaaac aattcggacg accaattggc
     2341 ggctttcagc ttcagcagaa aaaacttgct gaaatgctga cagaaattac caaaggtcag
     2401 ttgttggtat ggcggcttgg aaagttaaag aatgaaaaca gagccaatcc tgcacaaata
     2461 agtatggcta aacgtaacaa tgtacacact gcattgtggt gtgctcgcga agcgcgacag
     2521 atacatggcg gtatgggtat cacgggtgaa tatccgatta tgcgccacat gatgaatcta
     2581 gaatcggtaa ttacttacga aggtacacac gacatacatt tgctgataac aggaatggat
     2641 attactggcg agaatgcttt tacctgataa gggaaaagaa actaaagcgt tagtgcagta
     2701 gctgcagaaa tttttttgct ttgcttgttt tgaataaatg caatcagttg aaaattctta
     2761 ttcagttcaa gcttattggt ttttaatagc agtgttcctt ttgtggcaac aggatatact
     2821 tcgtaaagcc tcactacgtt gtcttgtacc aacgttcttc caatgttttc accttttgta
     2881 atttttgtgg taacactttt ttgaactacg gcaacaataa tattggcatt gatatctgct
     2941 ttgccggcag tgtagttaag gtgcaaggtg tcgttcaatg ttttctctac agtaattgtt
     3001 agtttttgtt tggaaacagc ttttaatcct ttttcaattt ctgaattcag acgcttgctg
     3061 tcggaaccta caaagcctgt gttgccgtta acaaacacct gtggtgtaaa cacttcattg
     3121 ctgcctgttg cactaacata attattctgt cgtcttgaat atttaatgct gctgtatgga
     3181 tctttccagc catatttatt ccagtagtca acatgaaagc ttaaaaaata aacaggtttg
     3241 ttattcttta cagcatcatc catttctttt ttcaaaactt tttctgctgc cggacaactt
     3301 ccacaaccct gtgaggtgaa tagttctacc acagcaaaac cacttcccgt ttgctgtgct
     3361 aaagcagttg atgacaacag tgttagaaga atgaataaaa atcttttcat gaatacttat
     3421 caataacgca aaaatgaaga aaattatatt agtttacatg tggtatccat gaagttataa
     3481 gcaagtagga tttgattttc aggcattatc tctccatcgc cacagataca tgcatgcata
     3541 agttctgtaa ggtgcccatt gctcagagat tgataatatt cgcaagcgca actttttctt
     3601 gtcggcaatt tttattttgt aaagtccgat tacagattgt tgaataccat aatcgtcaac
     3661 agaaaaaaca tcttcgcgac ctaaggtaaa catcattatc atttctgccg tccagcggcc
     3721 tacacctttt atctgtgtga tgtattcaat aaattcgtca ttactcattt tatttacttt
     3781 gttcaactgc aaactgtttt cctgtgcaaa tgcggcaata tttttaatgt aacttgcttt
     3841 tgaattactc aaaccaagac ttcttaaaga ttcagttgtt gcctcaagta ctttttctgg
     3901 agtaggcttt ccatcaaata aatcatagaa acgctgctta attacgtcag caacttttac
     3961 cgacaactgt tgtgatataa ttgaaccgca taaactcaaa aacaagtctt ttcttttttt
     4021 aagctccaca gttccatgtt gcttaattat aggtgctaat tttttatctt tagaaaggtg
     4081 tttgatatat tccatattca aaatttcggc taatgtatca cacttcaaaa aataatcttt
     4141 gtcctaacaa acttttaaca ttctattatt tagtactcaa caaatcacta aattaatttc
     4201 ggcttttctg ttctgtaatt tattctcccc ttccaacgct ccaaatattg taaacttcaa
     4261 atcttataaa catacactaa tattctttaa tttgcagttt cattagttaa tgacatgcag
     4321 ataaagaaat ttacatttaa tccttttgag gaaaacacgt atattgttta tgatgaaaca
     4381 tcacaatgtg ccattattga tccgggatgt tatatgcctg aagaaaaaga ggagttgctc
     4441 ggttttattg cagcacaaaa gctcaatcct gttttattat tgaatacaca ttgccatgta
     4501 gatcatattt tcggaaacaa acttatctat gatacttttg gattaaaacc acaatttcat
     4561 caattagagt taagattgct tgaagctgca ggtgaataca gtaagcagtg gggaatattt
     4621 ttggaaccat caccagctgc agaaaaatat ttgaatgaag gtgatgtcat ttcatttggt
     4681 aatgtttcat tggagatatt atttacaccg ggccacagcc ccggaagttt atgtttttat
     4741 cataaacaac agaaaataat tattggaggt gatgtgctgt ttcagatgag tataggtcgt
     4801 tatgatttgc ccggtggtga tgaacaaacg cttttaaata gcatcagagc aaaattattt
     4861 acattacctg ctgatgtaaa agtactgccc ggtcatggtc ccgaaacaac tataggattt
     4921 gaaaaaatgt acaatccatt tttagtttaa ggtgtaaaaa acaaagaagg cacgagcgaa
     4981 acgcccgcgc cttctttgtt tataaatatt acaactttct aaaccttcaa aactaaagtc
     5041 tatttcttgc actcatcaag cattttctgt gcaaactctt ttccccagtt tggcatcatg
     5101 gcattagccg gtttaaatgc attaaatttc tctattgcct tttcaagaat gggtaatgcc
     5161 ttggctttgc caccaccaaa ttgttcaggt gtaaacaaca gtcctgtacc ttgtagaaaa
     5221 tagggacgtg gattttcggg attcagttca atagcatgtg ctgtaagcat tcctgcttcc
     5281 atacccaact tctgtccgcg tgttgcaggg tcaataccaa ttttcataga ggttatccac
     5341 gattgcagtg taacaatctc tgaattatca ggcgaaagtt tatctgcttt gtttgcgatt
     5401 tcaagtgctt tgtctaaaac tgcatctttc tttacattgt ctttttcatc gtttgcagaa
     5461 agtactaaag cataagattg ataatataaa ggcaaccact ctttggtttc tgcattagca
     5521 atgcgctcaa atgcattggc ggcatttaca aagtcagtac ctgcttttgc tttatcaagc
     5581 aaatcaattt gtttaaccat tgttttttca aacttgctat tgctttgtgc aaacatgttg
     5641 cctgcaataa ggagtaaaag gaatgtgatt tttttcattg tatttataat ttaatttgaa
     5701 tttttttatt ctgttctgtc ttctccgaag ctgataaata ctcctataaa aaaggaacgt
     5761 ttagaagtcg cgccaacagc acttcttctc aacccatctg tgctgtagcg ataactgtat
     5821 gcactttcaa tacctaacac attgtttacg gcagcagata taactgtaaa gtgtctgcca
     5881 atggatgtta agtagctcca tgttaaactc aggttgttat agtcgttagt gcgatcggaa
     5941 agaaaattat tttcattggt tttatttggg ttataataag gtcttccagc tgcataaaca
     6001 taggtaaagc caatactgct tctcaatttt ggaaaccaac gtttcatcac tacactcacc
     6061 acatgtttcg aggcaaaagt gggttgcact tcaaacggat agtataaata tttccgctta
     6121 gtgtcgagga acgaatatga aacccaataa tctacatatt taaaggtttg cttatcgcgc
     6181 cagaaaaagt caattccttg tgcaaagcct tttccatcat caccggttgc aaatgctgtg
     6241 gcatacacat tcgatgccat attagttgtc tgcactaagt gattgtattt tttataatat
     6301 gcttcaacac gaaaggttct tttttcattt acatactgat agttcaagat gtaatgtgtt
     6361 gctttttcaa agtgatcaag gttggtaaag ataagataat ttttatcagg tgtctgatag
     6421 tagtcaccat aggcaacatt caactgatca aactttccta atttataagc aacactgatg
     6481 cgcggtgcaa gattacttgc acgaagcagg tatgagtttt ctactcttat tccggcacgc
     6541 gccactaatt ttcttgtgat gtaagcatcg ccctccagaa aaactgccgt aaacatttcg
     6601 gtgctgtcca ctctgaaatt attatagagg ttactgaagt cattatactt tacttctgcg
     6661 ccaccacgta tggagctcaa ctcactaata gaacgtgaca ctactgcacg accttgccac
     6721 agttgattac tgaaaagaag atgttgtggt ctggcataaa tacgattaat atccttgcta
     6781 taggatcctg ctgcctgcac agtccattta ttcaacactt ctttatatga taatgtggag
     6841 aagatgttgt agttattgag accaaaatga tttttacttt ttccggtaac gtcttccaga
     6901 ttttcgaagt tgagagagag gtcgctgtac tgataattga taaaagcttt gatgattcct
     6961 gtttcagatg tcttatgtct gaatatggca gagctgctga aagtttgagg aggagaatcc
     7021 cagtcgcggt tttgttttat tatggcaaaa tagggtgcaa ggttaaagta atttgcaaat
     7081 acacctgctg atgttttttt ccaccgatgc atgtagccac caccaccgcc aacagacatg
     7141 atgccaatgt ttccgataga acgtggtgga aggtcttcgc tttccaatgc cagcactgat
     7201 gacatggcct gaccgtattg tgcagaataa cctcctgtgc tgaagtttgt gcctttaaac
     7261 atgaatggtg aaaacctgct gcgttgcggt acatcaggca cacttgagaa atatgggttg
     7321 gcaacttcaa ttccatcaat gagtgtttta gcttctgctg catcgccacc acgaacaaaa
     7381 agtccttcct gatcctgcgt aggttgtgtg cccggtaatg tttgcaatgc tccataaata
     7441 tcaccttgcg aacctgctgt agtaacgata tctaatggtc gtaagatgac cattttcttt
     7501 tcatcacttg cttcaaaagc tccggctgta atagtaacag tattcagttc attacttgtt
     7561 tcttttagtt tgaaatttat ttcaatggca tcaccctgaa gttttatttt tttatctgat
     7621 ggactaaagc caatcaggct gcaaacgatt atcacatcgc ccgtctcatc agaggtgaat
     7681 tgaaattttc cggaggcatc gctactggca ccatcatagc tgtcgcggat aacaatgtta
     7741 gctcctgcaa caggattatt tttttgatcg gtgactgttc ctgaaatttt agtttgtgcg
     7801 gttactgatt gtatgataat taaaaaaaac aaccaactga ttacatgctt tgataaatac
     7861 atttgattcg gttatggtta aacaaaggta aaagatttaa ttgttttgtc aagctcttat
     7921 ttttttgtca gtgtctgtaa atattcctgt gcagtcttta ttaatttgtt gtaccatgct
     7981 tcgccatact ttctggtcag cgctgttttc agaaattcat acaccctcac tttttctttc
     8041 ttgcccaact tacatgcagg actgcatact tcccattcat gatagtttaa tgcatcaccc
     8101 gattttgttt ttaaaacacg tataggataa agatgacagc tgataggctt gtagaattta
     8161 atttttttat ctcggtatgc tttttcgatt ccacacagtg caataccatt ttcaaaaaca
     8221 gtgtaggcgc atggtccacc gggccacatc aatggtgtca cccattcatc atcatcatct
     8281 tttagataaa acccctgcgc ttcaattgct tttattccct cgtcagttaa ataaggttta
     8341 actttcggat aaactttttc caacgctttc aactcatctt cttccaatgg tgcacccgat
     8401 tcgccttcta cacaacatgc tcctttacac ttagaaagat cgcaaataaa tttctctgta
     8461 ataacctggt cagataccag tatgtcatca acttttaaca ccggtttatt ttttggcaaa
     8521 ggtaaaactt cgtggcattt tgaggaagga cgtcaaatat tataactttg cgcatcatga
     8581 atccaattat aacggtaaaa aatttagtca aacattacaa tagttttcag gctgttaaag
     8641 gaatatcgtt tgatgtgtat gaaggagaaa tttttggttt actcgggcca aacggtgcag
     8701 gtaaaactac aacacttgaa attatggaaa cccttcgcac caaaacatca ggtgagataa
     8761 ttattaaagg gtacagtgtt ggtgcagacg atgatgagat taaaaaaatt ataggagtac
     8821 agttgcaggc agcaggctat tacccaagcc tttcattact tgagttgatt gacttatttg
     8881 cagggttgta cagtcaggat gttaatgcaa tggaattact tctgaaagta ggtttaaccg
     8941 ataaggcaaa atcaaaatac aaagagcttt cgggaggaca aaagcaacgt ttttcaattg
     9001 caacaacatt aatcaacaaa ccggcagtta tttttcttga tgaacctacc acaggattag
     9061 acccgcaagc aaggagaaac ctttgggact tgattcgtca gataaaaaat gaaggcacaa
     9121 cagtagtcat tactacacat tatatggatg aagcagagga gttgtgcagc cgtgtagcca
     9181 ttatagaaaa cggaaaaatt attgccctcg atactcccga ccaactcatt gatgatttag
     9241 ttgcaaaagg atttgaaaga aaaaaagaag ttaaaaaagc aaatcttgaa gatgtatttc
     9301 tgaatcttac agggcaggag tggcgcgaat aatacacgct gcttaccgtt gatgcggtaa
     9361 ataatttttt gtgattttaa tggtaatact aaaatcaatc agcataatcc aatacatgtt
     9421 tttatcggtg taaacatcat aagttgtaac tccgtttttc tgctcaaaaa ttggttcacg
     9481 agttttagca taatagattc ctgcattggc accataacgc acaccaattc tgtcggtcaa
     9541 ctctgtttgc agatttaaac ccggcaaaag agcagctcca ttgtattgtg tagcatataa
     9601 acttccggga taggttgatt tttcgtactt aaagtttaca tatggaatat acagtgtagg
     9661 tcttatctct aatctttctt taaaaaacga cagtagcttt acagaaacac aagggccaat
     9721 ggcataattg cttacttcct gtgctttttc agagcgaaaa ttatacatgc cacgaatagc
     9781 gtaaatggtt ttgcgactac gcttgtttac actgtaatta agccagaagt tttgcgatcc
     9841 gtgaaatgat ggatagtaac caaaaccaat acgttgctct ttaaaaatat tatccttagc
     9901 acttatgctg cctgatgaaa gcagcaacaa taaacaaagt gcaaggccgg atttaagcat
     9961 atttaaattt tcgccaaatt ataaagttag aaccaaaaac aatataatcc tgaaaaataa
    10021 ttatctgtcc aaaacaatgt ttgtactttt gcaaaacaat gaatgagggc actttgcgga
    10081 atacattttc taaagcagaa cgactatgca aaaggaacga ttttgaactg atatttagtc
    10141 agggttcggc atttaaccgt tttccgttca gagtacattg gttgaaatta aaagaaaata
    10201 ctccatcatt tcctgttaaa attgccataa gtgttcccaa acgaaaaatc aaaagtgccg
    10261 tttcgcgcaa ccgtattaaa aggctaattc gtgaaagcta cagacttaat aaacagatat
    10321 tgagccgcga actgaaaaaa gatgagcacg tcatacacat catgtttgtt tataatggaa
    10381 atattaacgc taccttcaca gagatacaat ctaaaataat cctaatttta caacgtttaa
    10441 aagaattgaa tgagcaaact gctgaacagg attctgatag ccatagtaaa gttttataaa
    10501 atggccatat cgccattgtt gcctatgtca tgtcgttatt atccaacatg cagtgagttt
    10561 tgtatggagg cactgagaaa atacggaccg ttgaaaggtg gttttttatc actcaagaga
    10621 attttatcct gcaacccatt tggtggtcac ggtcatcatc cggtacctta atgattttta
    10681 aaaatattaa ttgattaaat agtttataag atgctacaaa aaatgcgtaa aagaatcaga
    10741 ttgtatgttg ccgttattct ggtcggaata tctgcttttt ctgtttatag tttcagtgac
    10801 gattattttg aaatatctcg caacctcgac attttcgcaa ccttgtttcg tgagttgaac
    10861 atttattatg ttgatgaaac aaatccgggc gacctgatga aaaagggcat tgatgatatg
    10921 cttgcctcgc ttgatccata caccaactat attcctgaaa gtgaaattga agactatcgt
    10981 ttcatgacta ccgggcaata tggtggcatt ggctctttaa tcggtcagcg caacaacgaa
    11041 gttataatta cagatcctta tgaaggattt cctgcacaaa aagcagacct gcgtgcaggt
    11101 gatgtaattg ttgaacttga tggtaaaccc attaaaggaa agaaaaatga ggaggtaagc
    11161 aagttactta aaggtcaacc gaaaactaca gttaaaataa ctatcagacg tgaaggtgaa
    11221 cccaatctga ttgataaaaa tgtagtgcgc gaagagatta aaataaacag tgtttcctac
    11281 agtggaatta ttgataatga tttaggatac atccgcctga caggatttac cgaaaatgca
    11341 gggcaggaag taaaaaatgc attacaagca ttggaggcaa agacaaaact cagaggatta
    11401 atttttgatt tacgtggcaa ccccggaggc ctactcaatg aagcagtaaa tattgtaaat
    11461 atttttgtga ataaaggagt tgaagttgta agtacaaagg gaaaggcaaa agagtgggat
    11521 aaaacctatc atgcattgaa caatcctgtt gatacagaaa ttcctgtggc agttttagtc
    11581 aacagcagca gtgcttctgc ttcagaaatt gtttcaggaa gtttgcagga tttagacaga
    11641 ggtatcatca ttggtcagcg tacttttggt aaaggacttg tgcaaactac cagaccgttg
    11701 agctatggcg cacaactaaa agttacaaca gcaaaatatt atatccccag tggacgttgc
    11761 attcaggcac ttgactatgc acatcgtaat gatgatggca gcgttggaaa aattcctgat
    11821 tcactcatca cacaatttaa aacaaaggca ggtcgtaccg tttatgatgg cggtggtgta
    11881 atgcctgatt ttgttacgga catcagacaa ttgagcccta tttcgcaaag tttattgctg
    11941 aaatatttat tgtttgatta tgcaactatg tatcgtaata cacatccgtc aattgctccg
    12001 gcaaaagaat ttcatctctc tgatgctgaa tatgcagagt ttgtgaaatg gatttctgca
    12061 aaggattatg attatgaaac caagagtgaa aaaattcttg atgagtttaa aacaacagca
    12121 gaaaaagaga aatattttag tgcagcagct gcagagtttg aagtgctgaa gaaaaaaata
    12181 tcacatgata aaaatgcaga tcttcaaaaa tttaaagatg agataacaga attgctcgag
    12241 aatgaaatcg tgtcacgcta ttattatcag tcaggacaaa ttgaggcatc atttaagaat
    12301 gatttggaaa ttaagaaggc tgcagaagca cttaagaaca aagatgtcta tagttccatc
    12361 atgaaaagct ctatggcttc ggttagagag aagcactaga atactgcatg tacaatgaca
    12421 ggccggctac aaagtataag taaacctcta ttttttgccg gtgttttact gtttgctgtt
    12481 ggattgccac tctccatgtt tttaatgagt gtgtcacaac ttttcatcat cgttggatgg
    12541 cttttggatg gagatttaaa ggaaaaatta aagacggcat ttaacaataa agttgtttta
    12601 ctgctggttg ctttcttttt atggcatgtg gtgggattac tttatactac agattttcat
    12661 tatggcttta aagatgtacg tgtaaaactt cctttgctta taattccaat tattttttcg
    12721 tcaatgcctg ttttttctga caagcaattt aaaacagtag cattggtttt tgtggctgcc
    12781 gtattggtat ctacattaat cagcatgtgt atttatgttg gtgttatacc aattaaattt
    12841 caaaatgtca gagatatttc aattttcatt tcacacatca gattatcact cctgatttgt
    12901 attgctgttt atttgtcatt ctggctattc agaagaatca agtttggtta tggattgtta
    12961 ttgttggttg tttggtttgt attctttctg actttgatag agtctgtcac cggtttgggt
    13021 attatggcag ttgtgttgtt aatttatgcc atcaatgctt ttctaaaaaa taaatctgtt
    13081 ttacgcaaag gagttattgc tttttcggtt gtagttgcag tttctttact tgctttcaat
    13141 atcaaaaaca cctggcatga tgtaacattt tttaatgatg cagagctgag aaatttagca
    13201 gcgaacacag caaatggtac tccatacacg catgacaccc tgaatcgtga agcagaaaac
    13261 ggacatccgg tgtggattaa tatttgtgaa ccggaattaa gagaagcatg gaaccaaaga
    13321 agccggatta tgtatgatga tagaaacctg aatggtgatg agattaagag aacaatattt
    13381 cggtttttag cttctaaagg attagcaaaa gatgcacttg ctattaaaca actaagtgat
    13441 gcagaaatta atgctataga gcatggtgta gctaatgcaa atgatttaac agattccaat
    13501 atccacaaca gactaagggc aacactttgg gagttgaaca gatataggac gggaggtaat
    13561 cccagtggac attcattaac catgcgtttt gaattctgga tgacagcgtt gcaaattatc
    13621 aaacaacagc cgttaacagg tgtgggcact ggtgatgtgc ctgatgcttt tgcaaaaaag
    13681 tatgatgaaa ccaattctat gttagatatt aaattccgat tacggtcgca caatcagttt
    13741 ctggctgttg ctgttgcact tggtattcct gctcttatac tttttatcat catgcttttt
    13801 tatccggttt ttcagtccca atactcaagg tcttttttat ttatgacctt ttggattaca
    13861 gcttttttat caatgctaac tgaagatacg cttgagacac aggccggtgt cagctttttc
    13921 atttttttta gttgtttgtt tttgtttctg aaaaacaaag agttagataa aaatattatt
    13981 tagaaacatt ctaaataata ttttctcctt actttcgcag ccgataattt tcattcaatg
    14041 aaaaactaca taatagttat actatttcta tttccattcc ttgctcagtc acagaccggt
    14101 gaggattcat tacataccaa tgcagctaat gaaaaacaga cctatatttc aggctatgga
    14161 gaagcaatgg tgagctatga ctcacgctac agaacagctc aggcaaattt taccagagct
    14221 gtagtttttg taggtcataa gttcagcaag aatatttctt ttctttcaga aacagaattg
    14281 gctgatgcac gcgttgaagg cggcaaggca ggtggagaaa tagcattgga acaagcttat
    14341 ctgcgttttc agttaaacag aaaaatgcat ttgcaggcgg ggttatttat tccacgaatt
    14401 ggaattatta atgagaatca tttaccaaca acttattatg gcaatcatcg tccctttacc
    14461 gaaacttttc tgattccatc aacttggcgg gagttgggtg ttgccttgta tggtagttct
    14521 gatgctgtta caggtttaaa ttattctata gctgttgtaa acggacttga tgcatcagga
    14581 ttcagtaatg gaacaggtat tcgcggaggc aggtttgagg gcggcaatgc aacagcttct
    14641 aatattgcgt taacaggctc agtacaatat gtaatgcagc attttacttt tcagatttca
    14701 gcatattatg gcggaagtgc cggactcact aaaagagaag ctgatagtct tcagttaaat
    14761 tcaggcatgt ttggcacccc tgtggggctt ggcgaagtga atgttcaata tcgcaaggac
    14821 aggcttacag ctaaagtact ttttgcagca atttcaatac ctgaggctga taaaattaat
    14881 cgtgcctatg ccaacaacac tgccaaatta tctattggcg gctatgcaga agttgcttat
    14941 cgcattctaa aaaaattgaa aacagatgct tttatccgtt atgaaaattt tgatcttaac
    15001 agcagcattc caaagaatgg aatcaagaac gaaatactaa aacaacaata tatcattgcc
    15061 ggattttctt gtttccccac accgggtgtt gtagttaaat tggattatgt atataagcaa
    15121 accggtgatc ttaatcctgc attaaacctg aatccatttc ccaatgcacc tgcatactat
    15181 acaacaaata gctttataaa tcttggctta ggctatagtt tttagaaatg aaacgcagaa
    15241 attttattaa aaacacatgc atgagttgtg gcatgatggc agcaggagga agcttactgt
    15301 cgttgctgtc atcttgtaaa agtacatcaa tattcaaaac agaagccgtg aatcatcttc
    15361 ttacagttcc actgtcagca atgaacgata aaaaaatatt aattgtgcgc aatgcttcag
    15421 cagagtatga tattttattg gtaaaaaaat ctgacaatct ttttactgcc ttagagatga
    15481 aatgcacgca ccgggcaaac ccactcaatg caagcgatgg cggactttac tgcacacttc
    15541 atggcagccg ctttgacctt gacgggaatg ttgtagccga acctgctacg cagccactca
    15601 aaaagtaccc ggtaatagta aacaattcac taattcaaat atcaatataa tttaatcatg
    15661 caatacaaat ttattttact ctcattagca tcagtgttgg ttttaagttc ttgtaaaaaa
    15721 gataaagaca gcagtgataa ttattcatca acacaaattc tgaatgactt ttcagttaat
    15781 gttgcacagg ctacttataa tgacttagcc tctaaaacag cacagttgca tcaagcaatt
    15841 acagacttta ctcaaaatca gactcaggcc aatcttgatt actgtaagtt attatggaaa
    15901 gagagccgcc aggcatggga gcagtccgaa ggatttttat tcggtcctgt tgcaacagac
    15961 gatattgacc cgcgtattga cacctggcct gttgatttca actctctcga ttcggtgctg
    16021 aacaatcaat ctgtatatga cgaaacttat gtcaattctc ttgaagatgc tttaaaagga
    16081 tttcatcctg ttgagtatct gctttggggc gttgatggaa acaaaacaat tttgcaattt
    16141 acttcacgcc agttagatta tttagaagcg ttggcagcaa acctaaagac actaacacag
    16201 caagtagcta caagttggac cacaggtaac tatcataatg aatttgcaaa tgcaggaaat
    16261 ggtagtcagg ttttcactac acagcatgct gcatttatgg aaatggttaa tgctatggca
    16321 ggtatttgcg aagaagtagc tgacggaaaa atggaagacc cattagtttc acaaaatgct
    16381 atgcttgaag aatcgccttt tgcaaaaaat tcaattattg attttacgaa caatattaaa
    16441 agtgtagaga atgtttatct tggaaaatat acttctcaag ggcaatcatt agagagtttt
    16501 gtaagagcaa acaatctttc tttagatggc tccattaaaa caaaaatcag tgcggctgta
    16561 actgcattag gaaatataac agttccgttt ggtcaagcca tatttacaca acagacacag
    16621 gtaatcaatg cgcagaatgc catacgtgaa ttgaaggaag ttttagaaaa tgatttagca
    16681 aatctggtaa gtcaaaaaat ccaataatct tgaaacggaa cattgttgtt gtaattattt
    16741 ttctgattct attcggagca cagtcttgca gaaaggccga agtgctttcg gaatcagaat
    16801 ggaatgagtg gctatcagga ggtaaacaaa cattgtttga cgagtctgcc ggagcattct
    16861 ccagcgtatt tcctgatatt tcagcacaca atgcaatgat tcatgaaatg ggcgataagg
    16921 catttgaggc aacatttgtc agtgcccctg cacctttgaa tcccggactt ggacctatct
    16981 tcaacaatgt ttcctgtgca tcatgtcata ttgctgatgg caggggtaag ccaccattaa
    17041 gcagtagcga accactctca gcaatgttaa tcagagtaag cataccaggt gtagcatcaa
    17101 acggtgggcc attggctgtg ccgggttttg gtgtgcagtt gcaacaacgt tccattaatg
    17161 gtgttgctaa agaagcagat gttatcatta attattctga acaaacattt tcatttcctg
    17221 atggtgaaac atattcattg cgcacaccaa cttatcagtt agcaaatagt tacataccat
    17281 tacccgcagg agtaatgctt tcaccacgaa tggcgcctcc tgtatttggt ttgggtttgc
    17341 ttgaagcagt tgacgaatct gaagtattaa aatatgctga tgaaaatgat gttaacggtg
    17401 atggtatttc aggcaaaccg aattatgttt ggaatgcact taaaggagaa cgcacattag
    17461 gtcgctttgg ttggaaagca aacaatccaa ctgtgttgca acaaacagca ggtgcctata
    17521 atgaagatat gggcattaca agttatgttt ttccggaaga gtcaagtaaa gggcaatctc
    17581 aatacgataa cagaaacgat gaccctgaaa ttcctgatag tgttttgcgc gcagtttcat
    17641 tttatgtgca gacattagct gtgcccgtaa gaagaagtgc agatgatgtc attgtgttgc
    17701 aaggtaaaaa actatttaac gatgcaaagt gtagttcttg tcacagacct gctatgcaaa
    17761 ccggtgttcg tgttgatttt ccggaagtaa gcaatcaaag aatttttcct tacacagatt
    17821 tgttgctgca tgatatgggc gatggtcttg cagaccacag acctgatttt gatgccaatg
    17881 gtaatgaatg gcgtacagca ccattatggg gtataggttt aacacagaag gtaaacggtc
    17941 ataactttta tttgcatgac ggcagggcac gctcactgca ggaagctatt ctgtggcatg
    18001 gcggtgaagc agagtcggca aaaaatactt ttaagaacat gagcaaaaac gacagacaag
    18061 cattaattaa atttttgcaa tcattataat ttcagaagtt taaaattttc tgacagtgat
    18121 gaattgattt tagatattct acaatcagta aaaaagcaaa gtgctttgtt tcgctttact
    18181 tttgatgctg aatctatagt attgagtgct gatatttttc aaataaaatc tttcatagat
    18241 ttcgggtttc gatttttaat ttttaggtat tctatcttag tatagagttt ttactgcagc
    18301 tagaacaaaa cattggcata agtcataata cttgcgccag taggaacgct gttgataaga
    18361 atttctcatt aattgatttt ttttcctgca cctgtaactt gcgttttggt tttttagatt
    18421 aaaatctaca atggtgtgtt ttatttttta cttttacacc aaaccgcaca gttatgcgtc
    18481 acctatcgca tattcctgtt ttaagaattt taataccttt tgttgccggt attgttgtag
    18541 gcatattcat ggctgtgcct gcttggttag ccattgcttt gttgctttca gggtttatcg
    18601 gagtgttagc ttttcatttc tcaaaatatt ttagccaaca ttttaaactt cgctaccttt
    18661 tcggaatagt aatacatttg tttttattct cctcaggcgt tttgctcaca caactaaaga
    18721 cgaaacttta cgacccttat cactacacac aattcgaaga tgctgagtat tttttacttc
    18781 gtattaccga gccctatcac atcaaacaaa aatctttaaa ggcagaagct gaaatagaag
    18841 cagtttttga aaacaataaa tggcggccat gcagaggaaa aacactgttg tattttgcat
    18901 ccgatgcagc agcaaaggca ttgcagtatg ctgataggat tgttaccaat gcaaagccac
    18961 agccaattgc tccaccacaa aatccagggt catttaacta caaaagattt cttggctttc
    19021 atcagataca tcatcagttc tatctgaagt cagggcgatg ggctactgca ggaaaaggat
    19081 tttctaatat tcttttgagt acggcttttg aattacggaa aaagtttttg aatgaaatac
    19141 aaggacaaat tttagtgccg caggaagttg cggtttgttc tgcattgtta attggtttta
    19201 atgatttgct tgatgatgat ttattatcgg cctatgcaag ctccggtgca ttgcatgtgt
    19261 tgagtgtttc aggactgcat gtagggttga tttttgttat acttcaaaga ctgattttta
    19321 ttcctgaaaa taagaaatat ctgaatctgt ttaaacaatt gcttattctg ttattgatat
    19381 ggttttatgc catgcttacc ggactttcac cttcagtatt aaggtcagca accatgattt
    19441 caattattat catagcaaag caactgggtc gtgataatta tatgcttaac actacgctgg
    19501 catctgcttt attgttgttg ttgattaatc cattttcaat tacagaagtt ggttttcagc
    19561 tttcatacct tgcagtcata ggaattgtat atctgcatca gcgattggtt acagttttgt
    19621 atccgacttc aatgctcatg aatcaggcat ggcaactgac ttcagtgtct ttatgcgcac
    19681 agttagtaac atttccgtta ggaattcttt actttaatca gttcccgaat ttgtttttgg
    19741 tttctaatct cttggtaatt cctatctcta ccggaattat ttgggtttct gttggtgcta
    19801 caattatatc ggctttgccg tatatgtcat cacttgtatt ttattcatgg aaagtaactt
    19861 ttgcactcac ctggttaatg aatcagttgt tactgttttt agagaacctg ccatttgcac
    19921 tgattagcgg attatatatt gatattgcag atacatggat tatctacggt ataatcgctt
    19981 ttgtagtatg gtttttaatt tacaactaca ggtatgcact ttatggagca ttaacattga
    20041 caattatttt tctaataacg cagttgtggc atgaatggaa tattctgcaa cagaaaaata
    20101 ttacagtata tgctttaaag aatagtacag caattgatat tgcgcaggcg agaaaaaggt
    20161 tagtcataac tccaaaacag ataatggagg ataaaagtct gttgcgtttt aatattaacc
    20221 gttattggta tgagacaggt aaaagagaaa tgcttcacat tccattttat caacaaccca
    20281 ctgaaagtaa gttaaaagaa atttttaaca gtaagataac cggaaatgtt gccactgttc
    20341 aagatggatt tattcagata gatagtagat tatattttat ccccggaaac tggctgccca
    20401 gaaaagtgcc attaagtaaa attaaagttg atggaataat tattacttca cgttttaaga
    20461 ataaactgcc aacacttttt aaatattttg attctgattt gttgattatt gatttaagca
    20521 gccgagctaa agttgatgcc ggtaaattgc ctgtcagttt gcaaatctgg gattgcagaa
    20581 agcaaggtgc agcacaagta aaaatttaag aaagcagtaa ataaaaaacc ggcaatccca
    20641 aaagcggaac taccggctgt gccaacatca aaaattattt aacttttacc ggaacaaaat
    20701 ccatatctag ttgaagaatt tcaatatcgt ttgaagtaac gggtgctgag cgttcaatga
    20761 gtagcacaca gccattaatc acctgtaaac tacgatactt ctgtccatcg cgtgtaatga
    20821 gttttactct gtgattttca ggattactta tgtttttaaa tgcatgatat aaataattga
    20881 gaaatctttc agggataatt atttgcttgt atgccatatt gataatgttt aagcgtgttg
    20941 tacggtaata cagataaaaa agttgcatga attttaccct tagtaactga taatagtaaa
    21001 gtgcggtatg attatctttg ccggcactaa aattcagata tgggatttat tgatgaactg
    21061 agatggcgcg gcatgttgca ggatattatg cccggtacag aggaattatt gaacaaagaa
    21121 acggttacag gttatattgg ttttgaccct accagcgatt cattaggtgt tggtaattta
    21181 gttcaggtta cgacattaat gcattttcaa agagcaggac acaaacccat tgccttggtt
    21241 ggcggtgcaa caggtatggt tggcgatcca agcggaaaaa gtgcagaaag aaatatgtta
    21301 agtgaagaaa tcctgcaaca caatttaaat tgtcaaaaaa agcaattaga aaaatttctg
    21361 gatttcgatt gtggaaaaaa ttcggcagaa atagttaata attatgactg gttcagagat
    21421 gtaaaatttc ttgattttat tcgtgatgtg gggaagcaca ttactgttaa ttatatgctg
    21481 gcaaaagatt ctgtaaaaaa cagattagag aatggaatga catttatgga aatggcttat
    21541 cagttggtgc agggatacga tttttatcat ttgtattatg ccaaaaactg taagctgcaa
    21601 atgggtggca gtgatcagtg ggggaatatt gttacgggaa ctgaactgct tcgcagaaaa
    21661 ggccgagttg aagatgcatt tgccttgaca acaccactga taaaaaaagc agatggcact
    21721 aaattcggta agacagaaag tggaaattta tggctcgaca gaaataaaac atctccttat
    21781 gcattttatc agttctggct gaatgcaagc gatgaagatg ccaaaaatta tatccgcata
    21841 ttttctttga agaatcagtt ggagatagaa gcattagagt ctgaacattt tgctgcacca
    21901 catttgaggt tgcttcaaaa atcaatagct aatgaaataa ccactcgtgt tcattcacaa
    21961 caggattgcg aaagagctat agaaatttca gaggtgtttt ttaatggaac agataaagaa
    22021 cttgcacaat taacagaggc tgatttgcaa gatgtcttta acggattagg aaaaaatgca
    22081 tttgaggttg aattgcaaaa aattcaggca ggcatgcagg cactggattt atttgctgca
    22141 gagacatcaa tttttacaag taaaggagaa gcaagaaaaa tgattacagc aaatggcgtt
    22201 tcattgaata aacttaaatt ttctgaccct tcacaaattg tcagtaaaga acaactgatt
    22261 gcgaataaat ttatactgat acaaaaaggc aagaagaatt ttttcctgat taaggctgtt
    22321 tgagtaatgt attataaacg ataattgtat ttgtaaaaat cacccttgcg ttagcagttg
    22381 aaaaacatct tcaagtcggc tttcttcttt ttgcattgac agtacagcta tattatggct
    22441 tactgccata gtgaaaacag ctttacgcac gtcatcatca ccactgtaat ttaccttcca
    22501 tgtatttccg gaatgatgaa taaccttact caccgtagga atatcttgta gccatttggc
    22561 atcaattttt ccatcaaact caactgttat ttcatgtacc ggtttggctt tttgacgtag
    22621 catgcctgta tcatcatcgg ccacaatttt tcctttgttg attatgataa cacgattaca
    22681 gatagcctca acttcctgca tgatgtgtgt agaaagtata acggttttgt gttttcctat
    22741 tgatttaatc agtgcacgaa tttcaaccag ctgattgggg tctaaacccg aagttggttc
    22801 atcaagaatg agtacagcag ggtcgtgaat taaagcttgt gcaattccaa ctctttgtct
    22861 gtagccttta gacagtgcgc caattttctt tttgtattca ggtgttagac ctgtaagggt
    22921 aatcatagtg tcggcacgtt ttgcagcttc tttagcagat aaataaattc caccaacaaa
    22981 ttgaagatac tctttaacaa acatgtcagg ataaagtgga ttgtgctcgg gaaggtagcc
    23041 aacattacgt ctaacctcta aggattgtga aagcgtatca aagccacaaa cagaaacttt
    23101 cccttcattc tgcggaataa agcatgagat aattttcata agcgttgatt tacccgcgcc
    23161 attgggtcca agtaaaccaa caacctgacc tgtattgatt tcaaaactta cattatctaa
    23221 tgcacgttgc ttgccataaa ttttggttac attttttact gaaacagaca tatacctcta
    23281 aaaaatttaa agttgtgcac taattctgtc caacacagtt tgaattaatt gatgcatgcg
    23341 ctcttcatat ttttttgaaa actgctgtgc aattctgttg gcaacaataa ggttaacagc
    23401 acagcaatga tgtcctaaaa tttttgccag accatacatg gcaccggttt ccatttcaaa
    23461 attagttacc ttatgctcac catgacggaa tgaatttaat ttttcaatca tatcggttct
    23521 tgctaattga taacgcaaca cccttccttg tggtgcataa aaacctgaac atgatgccgt
    23581 aataccttta tgcatacctt ctgaaagtgc atgttctaat tttaaagagc ttcgggtcag
    23641 atatgattcc ggcagtttgc aattgtttgg ataatgcttt ctgaaacttt caatgatagc
    23701 ttgttcttcg ggagtatttg tgtattgata aaagtttaat aaaccgtcta atcctaaacc
    23761 gaatgtcgaa aacacaaacg agtcaacagg aatttcgggt tgaagcgcac cggaagtgcc
    23821 tatacgaatt agattaagtg ctgttaaact gggttttacg gttcttgttt taagatcaat
    23881 atttactaat gcatcaagtt cattatatac aatgtcaata ttgtcggtac caatgcctgt
    23941 tgaaataaca gagattctac gccctttata atgacccgta tgtgttacaa actcgcgttt
    24001 ttgctttttt acctcaatgc tgtcaaaaaa ggaactgact tttgatacac ggtcggggtc
    24061 gccaacattt attactacat cagaaatatc ttccggtaga agattaagat ggtaaacgct
    24121 accatcaggg tttagaataa gctctgattc gggaatttga ttcataacaa ttgtaaataa
    24181 tttctgtgct aaggtaagat atattaaagc atcaggttaa aagtactgtc agttcatgta
    24241 atttacagtt aaataatcta ttggaagtca tattttatga aatttgatat ttcctttttt
    24301 tatatttgca tagttaatac acctgttttt tatgaaccaa gagaagaagg ttatagtcac
    24361 cccgatagac ttttcagaac aatcacttat tgcactttca caaacattta atcttgcaag
    24421 gttaaccggt agccatatca ggcttttgca cgttattgat caggactttg tttcacattt
    24481 aaccaacagc attttttcga aagaaaacta tgccgatcag attcgaaatg atattgaacg
    24541 cagacttgat gaactggcat caaaggtaca taaggaagaa aaaatttcca cttccaccca
    24601 tatcagaaca ggaaagattt ataatgaaat tgtagcggaa gccaatgacg ttgatgctgc
    24661 atttatagtt atgggaacac aaggtgccag cacattgaag aagaaatttc taggctccaa
    24721 tacttcaaga gtaattaagg aggctttatg tcctgtcatt actatcaaag gtcatgaaca
    24781 tcttaaaggg tgtaaagata ttgccctacc attggatcta acaaaagaaa cacgtgaaaa
    24841 ggtgaattat gccattgaca tggcaaagct ttttggtgct gttgtacaca ttttttcagt
    24901 gcttgaaaca gatgatgagt ttttagtaaa taaattggaa cgacaattgc agcaagttaa
    24961 gcagttcatt acggaatcgg gtgttgaatg tcaaggtgaa ttggttccac aaagtgattc
    25021 caatgtatca ttgagtataa tcgaatatgc aagaataaaa aattgtgatt taataatcat
    25081 catgacacaa ggcgaaacca atattgcaga tttatttatc gggacagcgg cacaggaagt
    25141 tattaataac tctgaaattc cagtcttatg tatccgacca atggttagaa aagacactac
    25201 acaatatgta ctatcataaa tccatttcct atgagcagca atcaactatt tgaaatcaag
    25261 agaattttaa ttccttatga tttttcagaa acagcagcgt taagtcttga acatgcagtt
    25321 ttcatggcca aattattaaa ggcagatatt tatctgctgc atattcttga atccgtttct
    25381 tttacctctg catttggtgc agcatttggg aatgtagata aaaagttaga gtcggaatcg
    25441 aataccaaac ttgatgaaat tggtaaacag attcacatga acaacggtat aacggttaag
    25501 tcaatcacag aggttggaag gatttatcgt aaaattgttg atgttgccaa acgcgaaaac
    25561 attgacatca taattatggg cacacatggt gtttcaggat ataaaaagtt taatgtagga
    25621 actaatacca gcaaagtggt tgaagaggca ggctgtccgg tgatttctgt acagacacat
    25681 agcaagaaaa tcggatttaa aaagattgtt ctaccaattg ataattctcc acagtcgcgt
    25741 caaaaggtaa gttatgcatt agctgttgca cgttcttatg gttcacacat ttgtattgca
    25801 ggtcttataa acttcggtaa tgaagacaat aagagaatgt ttaaactaaa agtggatcag
    25861 gtagaggaat ggcttctgca acatggagta agtgttgaga aaaaatttgt tgaaggcgac
    25921 aacctggcca aaatgaccat gcaagcagca gaagaatggg atgctgattt aattgtaata
    25981 atgacagaac aagaaccaag tattaccgga tttttccttg gcacctacgc aacacaggtt
    26041 gttaaccata gtaaaatacc ggttatggca gtgcatccgt cagaatcaga cggcagcaca
    26101 attactgttg gatattaaaa ataattttca tgatttacac atggggcgct taaggagcgc
    26161 ccccatgttt ttattacctt cgccaatcag aattttaaat actattaatg ccatcgttta
    26221 aaggtagtct gcgctttata tttaaaactt tactatggac tgcaggcata cttattatat
    26281 tatttctttt atttctatgg aggattcaga ttcccgatcc tgctataagc agtaaactaa
    26341 aaccaggtga ccttaaacgt attcaaacag gtgttgacag ctatcgtatc ggcaacaact
    26401 ggttgcgcaa aaatcaacaa ggtgtctggg aaatgtatat tgaaggaaat gactatgaaa
    26461 gaggtgttgt atatggcgta ttggcaaaag aattaattga gaaacaggaa gagtattttg
    26521 ttgatcagat caatgaaata attcccaaca aattttttct gcagtttatt aaaatgtttg
    26581 tagcatggtt taatcgtgat atttacaaat atattcccgc agaaaacctt cgcgaaattt
    26641 atggtatctc actttctttt tccgataagt acgattatat tggaccaaag tattatcgaa
    26701 ttctaaacta tcatgccgca cacgatattg gacatgcact tacagatttt caattagtag
    26761 gttgtacgtc atttgctgct aacgacagcc ttacggacga tcataaatta ctaatcggca
    26821 gaaattttga tttttatatg ggcgatgatt ttgcccgtaa caaactgtta ctttttgtaa
    26881 aacctgatag cggctactca tttgtttctt attcatgggc cggacttacc ggagttgttt
    26941 cgggcatgaa tgaaaaaggg ttaacagtta ctatcaatgc agcaaagtct gatatcccta
    27001 ccactgcaaa ggatcctatt tcattattag cacgcgaaat tttgcagcat gcaacaaaca
    27061 ttaagcaggc aattgcaatt gcagaaaaaa gagaaacatt tgtttcagaa tcgttgctta
    27121 ttggctcagc acaggatagc acggctgcat taatagagaa aacaccaaca agaatggatg
    27181 tgtatcattc accacacgaa ttgttggtat gctccaatca ctatcagggt gctgaattaa
    27241 aaaacgatcc acacaacatt tcaaatattg aacttagtga cagcaaattt cgatttgaca
    27301 gaataacaga attgctgcat gaaaattacc cgctcagccc aacaaaagca gcagctgtat
    27361 tgagaaataa aacaggaatg ggtaataaag agattggcta tggtaatccc aaagctatag
    27421 atcagttgat agcgcatcac agtattttat tcaaaccaca aagcagcgac ttttgggttt
    27481 ctacaatgcc ttatcaggaa ggcatgtatc ttcattacaa cctcgctgat gtatttaaag
    27541 gaaatatccg taacactgat tcactgaata ttcctgctga tgattttgta aattcagcag
    27601 acttgaaaaa atttgaagct tttaaagagg ttaaacagag aatttatcgg cacaatacct
    27661 ttggagtcag tcttaaactc agctcagcag aaatcaagga atatatcaaa aataatccga
    27721 tgagttatgt tacctatatg agcctgggtg attattttta ccatgaaaaa aattatacta
    27781 tggcagaggt atactaccaa gaagcattaa ctaaagagat agcttctaaa gcagaggaag
    27841 aaaaaataaa gcaggccatt accacctgtc agcagaaagc agcaaaatga cactactggg
    27901 aaatatagaa tttcagacga cagaagaaat tagaagtatg caacagcaaa ggcttgcaga
    27961 cttattaaaa tatgtatctg cacattctaa ttattatcag aatttatttc agcagtatca
    28021 tatcaacccc gataagattt ctttagataa tctgcatgag ctgcctttta caaccaagga
    28081 taatttggcc tgtagtaatg atgatttttt atgtgtggat aagaatcaga ttgcagactt
    28141 cgttactacc tcaggtacca tgagcgatcc tgttacattt tatctgactt cggcagactt
    28201 ggacagattg gcatataacg aagctatatc attgcaatgt gcagatggta gcagtaatga
    28261 tatttttcag ttaatgacta ccattgacaa gttgtttatg gcaggcattg cctattattt
    28321 aggcatcaga aagttgggag ccggcagcat cagagtaggt cccggtacac cgcatgcaca
    28381 gtgggagtac attcaccgtt tcaaacccac aacacttatt gccataccat cgtttatccc
    28441 acagctgctt cagtttgcaa aagaaaagca aataaacttt cagtcaactt cagtaaaaaa
    28501 aattgtttgt ttgggagagc ccgtgcgtaa tgccgatttt tcactgaacg agttgggcag
    28561 acgcattaca gagaactggg atgttaagtt gtactctact tatgcttcca cagaaatggg
    28621 ctcggcattt acagaatgtg gttatggcat gggaggacat tcacatccgg aattacttat
    28681 tctcgaagtg gttgacgaca atggggaacg ggtgcgtgat ggcgaattgg gagaagtagt
    28741 agttaccacc cttgctgttg aaggtatgcc attgattcgg tatcggacag gcgatttatg
    28801 tcatgtgtat tacaacaaat gtttatgtgg tagagcaact acacgtttag gtcctgtaat
    28861 gggcagaaaa cagcaaatga ttaaatttaa aggaactact ttgtttccac aaacaatttt
    28921 taatgtattg cataaaatgc cactgattga attatatcag atagaagtgg cacgtgacga
    28981 gtttaacaat gactctgtta ctgttttact gaatagtgct ttaaagagtg atgaatttga
    29041 acacaacctg ctgtcgttat tcaaatcaat tttgagggta acaccacata ttgcttttat
    29101 caccgaacag gaactccaca gcagggtata taaaacagat aaacgtaagc cggaaaaaat
    29161 tgtttatgta tgagaaatca gcgaaaggtt tttaatcata ctttttacat ggttatatct
    29221 taagatggaa gtgttcatta taaaactaac aaaatgatag aaaaagctaa agcgatatac
    29281 aggtttttat cgccaaaatc cctattcatt gatttgtttt ttggaactta ttccgttttt
    29341 aaaaaagggc gttatcgttc atattcatga catctatctt ccgtatgatt atcctcaatt
    29401 tatgtgtgat agattttaca acgaacagta tatgcttgct gcttttatat tagcaaatcc
    29461 tataaaatat aaaaccattt taccctgcta ttttattagc gaagacatag aattggcaaa
    29521 aactatttca cctgtatgga gtcaccctaa tttaacccat gttgaaaaac atggcggctc
    29581 ctattggttg atgataaatg agtaaatagt ttattatatt tgccgataac aattaataaa
    29641 aaatagtatg aaaaaatcgc tgcttacgtt aatgctttct gcattgatgc ttggtgcttt
    29701 tgcccaaggt aaaatgaatg aactttttaa gaacaatgta aaactcattt ttatgggtta
    29761 tgacctgtcg caggcaaggt ttatcggtac agatggtttt accgaccctg cagccatcaa
    29821 agataagttt gttgctgcat ggaacaatac ttttgtagct gagtacgaaa aattcagctt
    29881 acaaaaagca tttaaactca ccaacgacaa atacgaaact aatgtagatg tagtgcagga
    29941 tattaatggg aaattaaata ttcacgaaag agtgatgaac ggaagttatt caattggaga
    30001 agatgatgta aaaaaagcgg tttcgcatta taattccaaa gagaaagatg cagtaggttt
    30061 ggtttatgta gttgaaagtt ttaataaaac attagaaaaa gctgttatct gggtaacatt
    30121 tgttgatttg tcaaacaaca agatgcttta caccgaaaag atagaagaaa aagccggtgg
    30181 ctttggcttg aaaaactatt ggattgcgcc agcaactaaa ctgaaaaaag atattgaagg
    30241 aaaatactat aaaacctgga agaataaatt caagtaatta ggtgaataaa aattcatgct
    30301 ttcatttaag caggcagatt atcttgtttt gcctgctttc ttttttattg tcttcatgcc
    30361 gtggtttgtt tttatcaaag gagaaggcct atagtaaggc aattgcaacg aaaccatttg
    30421 atgctattat agtgccggga tttccttatt ttgaaggtca gccctggcca caggtagtgc
    30481 aaatgagaat gatttgggcg aaatttcttt atcagaacgg ctacactaaa aatattattt
    30541 tttctggcaa tgctgtttac tccccatttg tcgaaggaaa gacaatgaaa gcttttgcca
    30601 tagccatggg gattccggaa gaacatattt atgttgaaga taaagcagaa cacagtgttg
    30661 aaaatgttta ttattcatat cgtttggcaa aaaagttagg atttaaaaaa attgccttag
    30721 caactgaccc tgttcaaacc cgctcaatgg taagttttat tcaccgcttt agtctgccta
    30781 tctcatatct gccaataatg tacaaaacac tacgtttgtt accacataat gagcctgata
    30841 tagaaggtaa attgcagaga gtagaaaatt ttatttcttt gcctgagaaa gaaactttta
    30901 aacaacgttt tgcaggcact ttaggtaaaa atataaagtg gcatatcgaa gatttaaaga
    30961 agaaaagatt gcgtaaaaaa tattcacata atatagttca ggaatagaaa gatgagttca
    31021 gcagaaaata cgatgacaga aaaacgcgaa ttatcagcat ttgaagcaaa aaacgaagca
    31081 cagaaattag cgtttgcacc ggtagttttt caggtagtag tagctatgca acggttagaa
    31141 attcttgact taatctgcaa aaacagaaaa ggtatttcgc ttgaaagtat cagcgataaa
    31201 acaggagtga gtttatatgg cgtaaaggtt ttgttggaaa tggccgctaa tgctgatatt
    31261 gtaaaatata tagacgacaa tactgtaacc gttactatgc ttggttattt cctcaactct
    31321 gatgaaatga ctcgtgttaa tataaacttt actcacgatg tgtgttatga tggattcaaa
    31381 tttctaacag agtctattca gaatggtaaa cccgaaggat taaaaacact cggtaactgg
    31441 cctactatat atcctggtct ttcaagtctt ccggaacgcg ttaaaaaatc gtggtttgag
    31501 tttgatcatt tttatagtga tgaagcgttt agagatgcac ttaatatagt ttttcaagaa
    31561 aagccctcaa ctatttttga tattggtggc aacaccggca aatgggcgtt tgcctcttgt
    31621 gaatacaatc ctgatgttaa ggtaaaaatt cttgatttgc cacaacaaat tgctgttgca
    31681 aaagccaatg ctgaaaaaag aaatcttact gaccgcattt cgtactttga gattaatcta
    31741 ctcgataaaa gtcaaaaaat tcctaaaggt gccgatgtgg tttggatgag tcagtttctt
    31801 gattgttttg gtgaagatga aattgtagct attcttgaga atgtgagaca agctgctgct
    31861 ccacacacca cagtttttat tctcgaacca tttatagaca accaaaagtt tgatgcagct
    31921 gcctattgcc ttacggctac ttcgctttat tttactgcac ttgcaaacgg caatagtaaa
    31981 atgtacagtg taaaagcaat gaccggattg gttgaaagag cagggctaga agttgttgaa
    32041 acatttccat tgataggtga aagttttcat actatactca agtgtaaagt aaaagcttaa
    32101 aagtacggga gtgctgcatt cgcagcactc ccgtactttt tttatttatt caacgacact
    32161 aatttttcaa tatcattatt tacagaaagt ataagcaaag atgttgctgt acaaaaaaca
    32221 ggactggtaa tgcagtgatg tccattccaa ctgccatcat tgttctgaat atttaaaagc
    32281 cgtccactca cattgtcata ccatttatgc cattctttat ccttgctgat aattaaacct
    32341 tcaccggttt gcaggtagga tagaaactct tcaccaccat tgctgccaaa tccattcatc
    32401 acatcattac gctgtgcctg cctgctagca gcctgattaa tttcataagc agctgcatat
    32461 ttataagcat cagcttcatt cataccggca gcctgcaggc tttcactcgt tacagttgag
    32521 gtaggagata attttccttc ttttttagcc tttgctattt tatcttttgc aatactagcc
    32581 tcacgtgctg tgctgcgtgc tgtgcccgat atactgtaaa gcataactcc ggcaccatca
    32641 cctgttgcaa cagaatttgt ctttgcatca tagtttcctt tctgatagtt tttataacgt
    32701 tcaagctttt catcgtcaac agaggcacct gctgcttttg cactttctaa tgcattggtt
    32761 gcaaatgaag attgtagaac tcctgcccat cctgcacctt ttatacttcc gttttcactc
    32821 tgtgcatgct gtattttaac aacgcattta tcaagacttt tactcactct tgtttttaat
    32881 gtctgattgt tagtgatgta atccattaaa ttagtaagat actgtgtggc aagaactaca
    32941 tcaatatttt gaccaagttt gacttgtggt tgtgttgctg tttggttagt gatatttaat
    33001 gtttgtgcat cggctgcttc aatttgtttg agtaaatatt ctgttgctaa agaaagattg
    33061 gccgaatatt ttcctgtcgt tggcgtgttg cctgtccgca acaatgccat ggcaaccata
    33121 gcggttgttg cagggtctga actaactgca tgtgggtcaa ataccgattg cctggcatgt
    33181 gagcctgctc cccatccacc atcttttaat tgtgctttgc tgatccactc caatgccgat
    33241 gtcatagatt ctttaacctt taccggagtt gaataaataa agtttgagtc tattccttca
    33301 gcttcaaaaa cagaacggaa aacacatcct ttttctcctt tagtttttac tttgtaacgg
    33361 tcactgcctt ttttcattga tgtaagtaat gctactgttc cgatagcgag tagtaacacg
    33421 cccataattt ttatactttt catttcctta aatttttatt gatttcacta aggatgtaaa
    33481 agcccacatt atttcacaca tttatttgat aaaggcaaat gttattttca catttgcagt
    33541 atgaaacatg tcaatttatt ttctgttcct gttgcaatac tgtttttatc agctatcttt
    33601 tcatgcaaca cttcaaaaaa agtttctgaa aataattcag cagatagttt gcttataagc
    33661 ctgagtcgtt atccttgttt tggccgatgc ccttattatg atgcaacatt atataagagc
    33721 ggttatgcag tcatcaacag aaagggattt atggatagtc ttggaattta tgctacacag
    33781 attgaaaaac aggagttaaa tagtattatt gaggaagcaa agcaggctgg actgattgct
    33841 atgcccgata gtttttataa tcccggaatt gctgattatc ctgctactat cgtcaccgta
    33901 aatttgaatg gtaaagcaaa acgtattttt gatggtgtac ccgaatcacc tacttctcta
    33961 aaagattttg aagagaaggt tcatcagctt tttacagcat caggtcgtca atggaaacta
    34021 atgcaaaaac cagcacaaca gcaggattag aaagcattta tgccattatg tcgccaaata
    34081 ttttttggca tttcagttga catcagtttg ctgatgaggg ttctaaattc atgcaccaat
    34141 catataaagc atagttttta ggctaccatc ctttgcaaat cgctttgaat tgcagccact
    34201 ttacgttact tttgtcacct tttattttta gctcattata atcagcaaca atgattgatt
    34261 ttattaataa aactatttcc aaaatttttg gcagtaaggc cgaaagcgat tacaaagcac
    34321 tgtcacccgt aattgatgca gtaaatgccg tggccgaaaa tattgcaaca ctcagcaatg
    34381 atgaattacg aaataaaacc aatgaatttc gtacacgcat acaggactat ctttctgaga
    34441 ttgatgcaga acttacgcag ctaaacgaag aagctgaagc cgattctgaa atggatgcca
    34501 accgaaagga agaaatttat aaccgaatag atgctttaaa aaaagaaaga aataagaaga
    34561 cagaagaggt gcttaatgaa attcagcctg aagcatttgc ccttgtccgc gaagttgcca
    34621 aacgttttac agagaatgca gagttggcag tagaagctaa cgataatgat aaaaatctgg
    34681 taaccaaacg cgatggtata agaatagaaa atggtaaggc aatttacagc aatacctgga
    34741 cagctgccgg aagtaaggtt ctctggaaca tgattcatta tgatgtgcag cttataggtg
    34801 gttctgtttt acatcagggg aaaattgcag agatggcaac cggtgaggga aaaacccttg
    34861 ttgcaacatt gccaatttat ctaaacgcat tggcaggtgc aggggtgcac gttgttactg
    34921 ttaacgatta ccttgctaaa cgtgatgcgg agtggaatgg ccctttattc gaatttttag
    34981 gtcttacagt agattgtata gacaaacatc aacccaacac agaagaaaga cgtaaggctt
    35041 atttagctga tatcacctac ggaacaaaca atgaatttgg tttcgattat ctgcgtgata
    35101 acatgacccg tcatgcacat gaaatggtgc aacgaccaca tcactttgcc attgtggatg
    35161 aggtggacag tgttttagtt gatgatgcaa gaaccccatt gataatttca ggtcctacac
    35221 ctaaaggtga ctctcaggaa tattttgcat tgaagcctaa ggttgaaata ctgtttaatg
    35281 cacaacgtaa ttatatcaac actgtgattg ccgatgctaa aaagaaattg ggtgaaaaca
    35341 aacccgatga ggcaggcatg ccgttgataa gagcttacag aggtctgcca aaaaacagag
    35401 cgttggttaa gtttttaagt gagcaaggcg ttagagcatt gttgcagaaa acagaaaatc
    35461 actacatgca ggatcagtcg aaggaaatgc ataaagtaga tgcagaattg ttttttgtga
    35521 ttgacgaaaa aaataatacc gttgaactga cagagaaagg tattgaactg attactgcca
    35581 acaacgaaga tccacacttt tttgtgatgc ctgatgttgg agcttccatt gctgaaatag
    35641 aaaaatctaa tggaacaaac gaagaaaagt tagaacgcaa agaagcattg atgcgcgact
    35701 tttctgttaa atcagagcgt attcacacca tcaatcaatt gcttaaagct tatacacttt
    35761 tcgaaaaaga tgatgagtat attattgcag atggaaaagt aaaaattgtt gatgagcaaa
    35821 caggccgtgt acttgaagga cgaagatatt ccgatggttt gcatcaggct attgaagcta
    35881 aagaaggtgt aaagattgaa gcagccacac aaacctatgc cacagtgaca ttacagaatt
    35941 atttccgtat gtaccacaag ctggcaggta tgacaggaac tgcagaaaca gaagcaggcg
    36001 aattttggca gatatataag ttggatgtag tagtaattcc aaccaataaa aaaattattc
    36061 gtgacgacag agaagacttg gtttataaaa ccaaacgcga aaagtataat gccgttattg
    36121 aagaaattgc taaacttaca gctgcaggcc ggccggttct tgtaggtaca acatcagttg
    36181 aaatttctga attgcttagc cgtatgctca agcaaagaaa tataaagcac aacgttttaa
    36241 atgctaagtt gcataaaaaa gaagcagaca ttgttgcaga agcaggtcag gcagggacgg
    36301 taactattgc taccaacatg gcaggtcgcg gtacagatat taaattgggt cagggagtaa
    36361 aggaagccgg tggtcttgca attatcggta cagagcgaca tgactcaaga cgtgttgaca
    36421 gacagttgcg cggacgttcc ggtcgtcagg gtgaccccgg ttcttctcag ttttttgttt
    36481 cgttagaaga tgatttaatg cgcttgtttg gcagtgaaag aatagccaaa ctcatggata
    36541 gaatgggcat tgaagaaggt gaggttattc aacatagcat gatcactaaa tctattgagc
    36601 gtgcacagaa gaaagttgaa gagaataact tcggaatacg taagcgtttg cttgagtacg
    36661 atgatgtgat gaatgcacag cgcgaagtta tctataagaa aagacgtcat gcattatttg
    36721 gtgaaaggct tgcactcgac attatgaata tgcagtacga tgtttgcgaa agtattgtga
    36781 gcagctacaa tcaggagcgt gattttgatg cattgcagtt agaccttatt cgttaccttt
    36841 caatagaagc accaattgat gcaaaggctt ttttacaaat taagcttgaa gatttaacac
    36901 aaatggtttt tgaaaaagca aacagtcatt accggcagaa gtcaaatctt attgcagaaa
    36961 aggcattccc tgttattgaa aatattttca ccaatcaggg tcagacaatt gttgatgttg
    37021 ttgtgccgtt tagtgatggc accaaggcaa tgaatgtaat tgcaggactg aaaaaggcgt
    37081 atgattcaaa ttgcaaagag ttgatttctg ctctcgaaaa atcatcggtg ctcaatttta
    37141 ttgacgaagc atggacagag catttgcgcg aaatggacga cttgcgtcag agcgtacaaa
    37201 atgcagtgta cgaacaaaag gacccattgt tgatttataa gtttgaatcg tttgaacttt
    37261 tcaagcagat gattgacagg gtgaataaag aaattgtttc gtttttgatt aaaagctata
    37321 tccctgtaca aaatcaggaa aacgtacgcg aggctaaagt acaacagcaa cgtacagata
    37381 tgagcaaaat gcaaaccagc cacagcgaat cagattctgg aggacaaggc cgcccacagg
    37441 gaccaccgcc accgccaaaa cagcaacctg caaaagcaga aataaaggtc ggacgtaatg
    37501 acccttgccc ttgtggtagt ggaaagaaat ttaagaactg tcatggtgca ggtgtggatt
    37561 aactgctgca gtaagtaatt ttttttgact atgaaatcaa tttaaaattg cagccggcaa
    37621 cagcagtttt tattgttaat tttgcatttt cttattctat cttatgtcgc aagaattaat
    37681 tgagcagcat attcaggagg cagaaacatt ttctattcaa tcgcctgccg atcttgaaaa
    37741 cttccgcctt aaatatcttt ccaagaaagg tgtgctggca gatttgtttg cattaatgaa
    37801 aaacattgaa cctgccgaaa gaaaaaatta tggcttacag gttaatttgt taaaacaaaa
    37861 gacagaagcc atcttcgaaa gttttaaaga aaagtttgag aatgtaactg ctgaggagaa
    37921 gaagatagac attaccttac ccggtgagcc aatgagtgag ggagctttac atccgctatc
    37981 cattatcaga aacagaatta tcgaaatttt cagtgctgtt ggattcaccg tttctgatgg
    38041 tccggagata gaagatgact ggcataattt tacagcactt aattttcctg aagagcatcc
    38101 ggcacgcgac atgcaggata ctttttttat tcgtaaaaac cctgacctgg cattacgcac
    38161 acatacatca tctgtgcagg tgagaatgat ggaaaaacac aaaccaccgt tgcgtacaat
    38221 ttcgcccgga agagtttacc gcaatgaagc catttcagca agggcacatt gtttttttca
    38281 tcaggtagaa ggactctaca ttgataaaga tgtttctttt gccgacctga aacaaacatt
    38341 actctatttt gccaaagaga tgtttggcgc agacactgaa atacgtttac gcccttcgta
    38401 ttttccgttt acagagccat cggcagagat ggatatttcc tgtaatttct gtaaaggcga
    38461 aggctgcaac atctgtaaat acacaggctg ggtagaaatt ctgggttgtg gcatgattga
    38521 tcctgccgtt ctcgacaact gtaaaataga tagcaatgtt tacagtggct ttgccttcgg
    38581 tatgggaata gaaagaatta cgatgttggt ttataatatc aacgacctgc gaattttttc
    38641 tgaaaacgat gttcgttttt taaagcagtt tagtggaata gggtaaggag agttgcttta
    38701 tctcaacaaa ttaaaagttc ctttcttact cacaattttt ccattcagcc aaccttcggc
    38761 tttggctgta taaacatagg tttctacatt ctgtaactga cctttaaaat aaccatccca
    38821 acctttgttt aaatcgttgc tttcaaaaat caactgaccc caacgattgt aaatcttaaa
    38881 ttctatcagc cgttttagtc cgagtccatt tacataaata acatcgttat cgccatcacc
    38941 gttgggtgag aaagccgtag gtacatcaat ggttattctt ggatctattt ctatgttgaa
    39001 tcttgatata ccggaaaagc aacctgcaga atcagtaaat gttgctgtgt aaagtgtgtt
    39061 caccaaaggc tgcgcccatg ggtcaggaca attaacacaa ctcaatccgt taacagggtt
    39121 ccagttgatg attaaattat ttgtaagccc tgtatttaat tgtatagaat ctccaacaaa
    39181 aagcaaatgc gttacattga cttgaggagg cgggctgtaa attgttactg gtgcagtagc
    39241 aactcccgta catccgttta catcagttat caataccgta tatacggtgc tttgacctgg
    39301 tgtagcaacg ggattggcaa tagttgtact tgaaagtcct gctgctggcg accacagata
    39361 acttacacca cctgatgagt gtaatgttat tggtttgcct aagcaggtgt cgccaccggt
    39421 tgcagatact ataggaaggt tgttgatgct gatttttttt ctgatagtat ccttacatcc
    39481 ccatgttgaa ttatcaatgg ccagtgtaat ggtgtaaacc cctgtttgat tgatggtgta
    39541 agacggtggt gtagcaccat taaaagtttg tccgttgcca aagttccaat agttgttgct
    39601 tgcattcagt gaagtgttca ctaaggttac aggttcgcca acacataatg cagtgtcgcc
    39661 actgttaatg ttgaagttgg caataacttt tcggataaat accggatttg tttgtgtgta
    39721 agtacaagcc gaatcagcag accacatcac caaacccaca agtgtttgtc cgctgacagg
    39781 atttatgttg tatgtatgtg ttatgggtga tactcccgga gaggtcatac catcaccaaa
    39841 atcccatgaa aatgcaccaa ggtcagctgt atcttttata ctgaaagtaa tggattgccc
    39901 tttacagatt gttgaaggtg caatgttgaa gtttccaaca ggcccaagca cacgaagtgt
    39961 atcataagcg gtgtctctgc aaccaaatgt agtctcttca ataagttgaa tgtagtaaat
    40021 gcccggtgtc tgataaattg ttcctgcaac aggcgttgta aaaatgttac tgctgttgcc
    40081 aagattccac ttgcggctgc caaaaacact tacaatactt gtatcataaa agttaaccag
    40141 caatggataa catttattga gcatactatc tgcactactt gtaaatcctg cttttggaaa
    40201 tgcttgtaca ttgatgtagt tagtaatctt tttacttccc gggcaaccta tggaatcagt
    40261 aacagataat gttacagtgt aggcacctgc tgcattgtaa gcatgtgatg gtgatacctg
    40321 tgtagatgtg gtgttgtcgc caaagttcca cgagtatgat gaatgaccgg taaaatgtgg
    40381 attgaataca attgcttcac cgacacaaat atttgttttt gtggaagaga ataacgtatc
    40441 aggcaatgac gcagtaattg tcttttggaa aacactggtg cagccaatag tatctgtaac
    40501 aatcagtgtc acagtccagc ttccacctgc aggtgcagta gtgtaggtgt gacttgggtt
    40561 cttaacagaa gaagtgtttc catcaccaaa atcccatgag taagaagcta tggttgtatc
    40621 agcaaaacta ctgtcggtaa aattaattgt aagcggaagg catccgtagt ttttattggt
    40681 tttaaaataa gcattggaag aatacacttt tatttttctc ttcacagagt caacacatcc
    40741 gttgatgtcg gtggtaacta atttaataaa ataaacacca ttgttcaggt aggtatgtga
    40801 agctatgggc gatgctgaat tatgcggaaa agcatttcct ccccaatacc ataaaaaccc
    40861 ttcattacaa ctgccatatg catcagtgga tgaagaagca tctacattgg cagtatatcc
    40921 tttgcagatt gcagaatctc ctgtaaagct tgctgataaa tttctgacac gaacaatatt
    40981 ggaatcaata tcaggtgcac aaccggatgt tgtattgaat gaagttaatt ttacccaata
    41041 gtcaccagta gcagcatagg tgtgtgtcgg atttatgttt cctgaattaa ttaatgccgt
    41101 gccatcacca aaatcccaat tccatgtagt tgcatcgtta atggttgcag ggaaactata
    41161 cttaaatggt gtggcacaat ttcctgctac atagaatttc gccataggtc ctttaacagt
    41221 gacagtttgt gtggctgtgc tgacacaacc attgtttgat gtagaaagtg taatggtctg
    41281 ttgaccggtt tgtgtgttga aattccaaac aggattggtc tgattatagc agtgagacaa
    41341 catgcctcca tcacctgaaa aatgataaat attattggtg tcgggattta gaacattaaa
    41401 ttgcacaggt gtgtaggcac atattgttgt aggtacaacg gtaaaagctg ttgtgacggc
    41461 agaacctaca taaataggaa taacaaaaga agtgtctttg catcctgctg aatttgtaac
    41521 tactaatctc acatagtaaa tacccggtgt attgtaggta tgcgaaacca caccattgct
    41581 gcctgttgtt tgtgtaccat caccaaacag ataggtgtaa tttacaatag tctcccgcga
    41641 acggcttgag tcagaaaaaa taactgtcat cggtgcgcag ccaatggatt tattgggcat
    41701 gaaacgcgca gtgatgctaa aaacagtgtc gtttttttgt tttgcaactg tggcagtaca
    41761 accatgtaca ctcgtaaaag tcagttgatc ggtgtagtag taatagtcat aaatgg
//
LOCUS       DAOQNP010000017        39790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_307_length_39790_cov_10.186460, whole genome shotgun sequence.
ACCESSION   DAOQNP010000017 DAOQNP010000000
VERSION     DAOQNP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 39790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39790
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_307_length_39790_cov_10.186460"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2848)
                     /locus_tag="PKY09_04030"
     CDS             complement(<1..2848)
                     /locus_tag="PKY09_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNB12055.1"
                     /translation="MNKRFYFLLLIFTPMMLWAQNLKPVAQKIFTAKQQQAVFATENL
                     FTISPDNEQLNRQYAPTATKATFVELNKSAIATLMSQQPDYLSLRIPINNNVVQVDLY
                     KANIFTPDFSVTSAGSNGGAVNYTPGLHYQGIINGDNNSIAAISIFDDEVMALISTPA
                     DGNFVIGRLENNRSNLHVIYNDADLTSRPAMECATLADNGTYTPQELQLPANRGMMVN
                     CIRLYWEVNYNIFQGKGNITAAANYVTGIFNQSAIIYTNDNIPVELSEVYVWDVVSPY
                     TGTTSSNYLSQFQTYRTSFNGDLGHLLGYGGGGGIAAGFSGICASNINSSKCYSGINS
                     SYSNFPTYSWSVMVVTHEQGHLMGSRHTHACVWNGNNTAIDGCGPAAGYGYEGSCSGA
                     PIPSGGGTIMSYCHLNSVGINLSLGFGSQPQAVIINKYNSGACLTACIGMACMPPPGM
                     NTSSITSTSAIFNWTAAASASSYVVRYRVVGTSTWTTATASASPYTATGLTPGSNYEW
                     QVQTVCSGGTSIYTISTTFVTPPTTCNVPSGTVTTNINATSATYTWTAVGGAVSYNVR
                     YRLVGASTWTTANTTANSYSVYSLTASSNYEWQVETVCAGGTTSGFSASTPFSTTAQP
                     CNTPQNNYTTSISSTSAVLYSSGSAGSALNLQYKKASNSTWTILNNVSTPYTLTGLQP
                     NTNYQWQVQLVCGLAVSPWSSTVPFTTLCDTPTAVISPSGTINVCAGPVVLSANTGTG
                     LTYQWLNNGNVISGADNPTYSATATGNYAVVVTNSNLCSSTSAIVSVSIGAMTGQITP
                     SGTVSFCAGSNATLNATSGPGFTYQWYRNNVLLSSTNSSINIGSSGTYYCVITSGSCT
                     VTTNSVIGNQINNPTPSISHTTPTSFCSPGSVVLTANSFTGVQYQWQKNSVDILGATS
                     QNYTATSNGSYRVIETANGCSKSKTIAVNAASSVS"
     gene            2929..4239
                     /locus_tag="PKY09_04035"
     CDS             2929..4239
                     /locus_tag="PKY09_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNB12056.1"
                     /translation="MNTRIKRGILIICLLLSKSLFAQNLYFPPLTGNNWDTLSPSALN
                     WCQPRIDSLYQFLEDRHTKSFIILKDGKIILEKYFGTYTADSIHYWASAGKSLTAFLT
                     GIAQEKGFVNIQSPVSQYLGTGWTSAPPAKENLITLRHLLTMTSGLDDSPTPPCDVND
                     SSKTCLNYLADAGTRWAYHSGAYKHLQDVISVTSGMSYNAFTTNYLSSKIGMGGLWYD
                     AVFYSKTRGMARFGLLTLSKGIWNNDTILKDTSYIAAMSNTSQNLNNSYGYLWWLNGK
                     ASFMIPQLQVVFPGAMLPAAPPDLFAALGKNDQKIYVVPSENLVVVRMGDSAYSSQYA
                     VTVFDNELWTYIDSLSFCPTALNEINTNQHTWIYPNPANNFLQLDSETSTYFIYNIQG
                     RCCLQGKPDAQRKIDISMLQEGVYYLKIQTKNNQQWYFKFIRKE"
     gene            complement(4243..4422)
                     /locus_tag="PKY09_04040"
     CDS             complement(4243..4422)
                     /locus_tag="PKY09_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12057.1"
                     /translation="MKHFINKKTINPFNKKELIALSKMWKLKLSQLKDLMGVTGSARL
                     DVLENHLRKTGLWQL"
     gene            complement(4873..5649)
                     /locus_tag="PKY09_04045"
     CDS             complement(4873..5649)
                     /locus_tag="PKY09_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12058.1"
                     /translation="MKKTIGTLLLMTLTTCLLAGDKVIYSVNFKKSETFKVSGGVFNS
                     EYWEVRNDSAIFTSNNRLCNEKGNYRYQVEVTLTADGKLNSDDQCIIYIMQNEAIAKS
                     FVVKGSDMTESKKFKYEVEGEKSPVLKVNIVLKSLGVDRAWRLTEGAVAITAISKDDD
                     PELKVIQHANTSSVVWKCKTNDSNNYFIVERSANGTDFSEAGLVKANRNNQINYYSFV
                     DRTDAGTTNYYRIKLKSFDGDETQYGEIVKAGKEDVANSK"
     gene            5731..6858
                     /gene="hemW"
                     /locus_tag="PKY09_04050"
     CDS             5731..6858
                     /gene="hemW"
                     /locus_tag="PKY09_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNB12059.1"
                     /translation="MAGVYIHIPFCRKACTYCDFHFSTQLQNKNAFVEALLHEIELQT
                     HYINEPVTTIYFGGGTPSLLTAAEINTIIEKLNTVFKLDVKEITLEANPDNLTTVYLK
                     ELQTTAVNRLSIGVQSFEEQRLQWMNRSHTTQQSVVAIESALQIGFNVSADLIFALPE
                     MSNDEWQKQLQKMIGLKPHHVSAYSLTLEERTVYHHQVAKKKIKELNDVQSEAQFMLA
                     HQLLSEAGYHHYEISNYASDGHVAVHNSSYWKAEIYLGLGPSAHSFNGKTRQWNIRNN
                     TAYIKSLHEGKLPFESEMLTEVDSYNEYVMTRLRTDKGIDEKQWHKLLPENHKVNLLA
                     EMNELTGQKFLVRDASRIFIPLQHWYKADQIISRLFATEIS"
     gene            6864..>7582
                     /locus_tag="PKY09_04055"
     CDS             6864..>7582
                     /locus_tag="PKY09_04055"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNB12060.1"
                     /translation="MIVHLKIGNQEYSAKLNEPIDISIPLHSGNDNVNAWYAPPPEIV
                     PVRMDSWVGEVAQGAAVNFRNVFFNPHAHGTHTECVGHITKEWVSLNKVLKQFFFSAK
                     LITVLPKIADGDRIITKEDIVQAWENCGAEALVIRTLPNDNSKQCRHYSNTNPPYLHH
                     EAAAWMAEQNIKHLLLDLPSVDREQDGGKLLAHNAFWNTSKNVRYGCTISEMIFVPDH
                     IADGLYLLNIQITALENDASPS"
     assembly_gap    7583..7682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7683..8021
                     /locus_tag="PKY09_04060"
     CDS             <7683..8021
                     /locus_tag="PKY09_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNB12061.1"
                     /translation="LERMLERTPLLKIIYTSVKDLIEAFVGEKKRFNQPVLVTVDKVS
                     GVQRIGFITQKDLSVLGISSNKMAVYLPFSYGFNGQLVIVPAENVEKIDASGTEMMKF
                     VISGGVTEVD"
     gene            8065..9630
                     /locus_tag="PKY09_04065"
     CDS             8065..9630
                     /locus_tag="PKY09_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12062.1"
                     /translation="MLILIGSGVCAQDYFIPLNREWNLRYEPFLQTTKSNKLHTSIRP
                     YRNDEIIAVANIDSIDTANFPITRFSKTLVGRKIFKEHLVQIKDDDFLIHADFIFEGR
                     IGKDLPETETLFTNSRGFWLGGTAGKRFSFSTTFFENQARFPAYIDTFVTHYSIIPGE
                     GRIKKQYGSYDYSNVTGTISYTINRHFNVQFGHDKNFIGDGYRSLLLSDNANSYPFLK
                     ISTTAWKVKYVNLFTVLTDMQFPAPNDIAFKKKYASFHYLDLNIGKHASFGILESVVW
                     SGDSARGQGFDINYMNPFVFIRPVEFSIGSPDNMMLGFNLKVKLFSKLVFYGQLMLDE
                     FKLSEIRDGNGWFANKQAFQLGLKSYDLPIKNLDVLTEFNYVRPFTYSHRTTFTNYAH
                     FNQSLAHPLGSNFIESVSLINYKYKNFIFRFQFNYAETASDTGNANLGNDIFKSYETY
                     TFAYGNRMLQGQRHNLIYSDIRAGYLINPRTNLCFEIGYSFRRMTMAKEAATTNWVYA
                     GIRTALFNRYHDF"
     gene            9739..10809
                     /locus_tag="PKY09_04070"
     CDS             9739..10809
                     /locus_tag="PKY09_04070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNB12063.1"
                     /translation="MINIAFSCITAFIITFLAIPSIIKVAVIKNLFDEPGGRKAHKAS
                     VPTLGGLAIFAGVVFAFTFWSAGFNLQDTQYIIAALIVMFFIGIKDDIVELPAMKKLY
                     GQIVAAVIIVLFANIRITGLYGIFGIDQVPYWFSLLFSMFTILLIINAFNLIDGVDGL
                     AAGIGAIAAFTYGIYFYNYNEIALCILSFSLFSSLLAFLVYNFSPANIFMGDTGSLII
                     GLILSILTIKFIEMSFAPRPENFPFQSSPAMAVAILVLPLFDLLRVFIVRILNKRSPF
                     EADRQHIHHLLLDMGLNHREVSITLYLVNIGFILVALALRSFGALALLFIISTLALIL
                     SLVPYYLKYQAGRSGAVSRSHK"
     gene            10912..12516
                     /locus_tag="PKY09_04075"
     CDS             10912..12516
                     /locus_tag="PKY09_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNB12064.1"
                     /translation="MTGIFPDSLPAKIIIMRLKKLVYSIALLLPFIGNAQVVENTQPQ
                     SGDPIAAMLDSLVSIRNVVRLSETNLQQPPVFSENDAVLSDAFIQERISKISTPIPLT
                     YNYQVKNYISLYASQRAQLTSRVLGLSRLYFPYFEQMLDKYNLPVELKYLAIVESALN
                     PTAVSSAGATGIWQFMYNTGKLYGLNINSYIDERRDVRLATDAACRYFQDMYAIYGDW
                     LLVIASYNCGPGNVNRAIARAGGSKNFWEISRFLPAETRGYVPAFIGATYVMYYAKDY
                     NITPTTPTFTYFDVDTVGVKNNISFRQLSQTLNIPYDVIAFLNPVYKRNMIPGEGNRL
                     YLPTEKISLYLANEESLYASSGTELQPSSFAIADDVGYVQVKQHVKVRKGENLYSFAK
                     RNYCSAKQVKKWNNLRSNYLKAGQKLVVYTTIKQKATPVETTTETAANDKTDSAKVNT
                     AVKADSSANTETGAVTSESKTTQVNQTQSAASKCIIHLVQPGDTLWNIAKRYEGVTVE
                     LLKEVNNFTPQSTLKVGSKIKIPTVG"
     gene            complement(12535..13056)
                     /locus_tag="PKY09_04080"
     CDS             complement(12535..13056)
                     /locus_tag="PKY09_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNB12065.1"
                     /translation="MNILDLFLAVPLLWGAYKGFTRGIIYEIAMILGLIAGLYVAFKF
                     SGVVQGWLFQLVNKDSALPAWVGFFIISGIIMAVFILYARLLEGILKAGDLNLFNKII
                     GGVFGLLKYALVLSVFLWLLKSLEPHLNFINSQTKKESKLYDPVLKTSTFLTPALQDI
                     KNEFRQHIEDDKH"
     gene            complement(13153..14076)
                     /locus_tag="PKY09_04085"
     CDS             complement(13153..14076)
                     /locus_tag="PKY09_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNB12066.1"
                     /translation="MSLLVVGTVAFDAIETPFGKTDKIIGGAATYASLAASYFAPKIN
                     LVGVVGDDFPQSTIQDFNNHNIRTEGLQIIEGEKSFFWSGRYHTDMNSRDTLDTQLNV
                     LANFDPIIPDSYQDCEFLMLGNLMPKIQLQVIERLRKRPKLIVMDTMNFWMDMAMDDL
                     LKNISMIDVLTINDAEARQLTNEYSLVKAAKKILNMGPKYLIIKKGEHGALLFHNNEV
                     FFAPALPLEDVFDPTGAGDTFAGGFIGYLAKTRDISFSSMKRAIIYGSAMASFCVEKF
                     GTERIMNLDSDELNERIQEFIDLVQFDITLV"
     gene            14247..14792
                     /locus_tag="PKY09_04090"
     CDS             14247..14792
                     /locus_tag="PKY09_04090"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNB12067.1"
                     /translation="MFEQLLNLVKENAGEAIINNQAIPNEQNDAAINQATEGIIGGLK
                     SQLASGNISEVAQLLGGQNNAATSGVTNAISSQVSQGLMEKFGLSGNSAGSIVSSLIP
                     VVLSKLVSKTNDPNDKSFDLNSIFNHLSGGKTQGMDLSSILGSVTGGNTSGGGIGNVL
                     GSVMGGDKNSALGGLMDIFKK"
     gene            complement(14948..15382)
                     /locus_tag="PKY09_04095"
     CDS             complement(14948..15382)
                     /locus_tag="PKY09_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNB12068.1"
                     /translation="MNSIFGNYTLQDVKKLERNTMLSHLGIEITSIDNNAVKGKMSVD
                     HRTCQPMGILHGGASCALAESLASIGAFLTLKDDTYVAVGMEINANHVRPVKEGFVYG
                     TAKPFHLGKKTQVWTIEILNEKNQPVCISRMTIAIGRLQLKV"
     gene            complement(15382..16002)
                     /locus_tag="PKY09_04100"
     CDS             complement(15382..16002)
                     /locus_tag="PKY09_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12069.1"
                     /translation="MFVLLSCSTNVHSQLRDSLAYLLKQRPKPFFQLDGYNSFVRSRG
                     ANAIGFKTGLDFGKRVKFSMGYMKLFTDVVDSVQIRPNKYYRGEVRSNYFTTGIEYVL
                     YRNGPWQVNVPMHFGFGTSYYEYPDGKKANNKALQGNIILFEPAITGHYKLIRWVGIG
                     FGVGYRVMLKNNKQLKDNLSSPIYILRLKIFVDEIYRTVFPSKKSK"
     gene            16238..>16692
                     /locus_tag="PKY09_04105"
     CDS             16238..>16692
                     /locus_tag="PKY09_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNB12070.1"
                     /translation="MKVEIKVPSPGESITEVQIARWIKKDGDYVERDEEIAEIDSDKA
                     TLSLVAEADGVLKTLVAEGENITVGSVVCTIDTDAKPVAKAEPIKEEKPKEEKAKEVK
                     PAATAIPSKAEGYAAGTPSVAAAKMMKENNVKGESVQATGKGGRITKGDV"
     assembly_gap    16693..16792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16793..17323
                     /locus_tag="PKY09_04110"
     CDS             <16793..17323
                     /locus_tag="PKY09_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNB12071.1"
                     /translation="KAVCEALKSFPAVNAQIDGEEILYHNYCDVGIAVSAPKGLMVPI
                     IRSAEKLSLAQIEQQVIDLATKARDSKLTIDEMTGGTFTITNGGVFGSMLSTPIINPP
                     QSAILGMHNIVERPVALNGEVVIRPVMYVALSYDHRIIDGRESVGFLVKVKQMLEEPA
                     KMLFEGNDPIKVLLHL"
     gene            complement(17400..19559)
                     /locus_tag="PKY09_04115"
     CDS             complement(17400..19559)
                     /locus_tag="PKY09_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNB12072.1"
                     /translation="MNYKLRLFFTACALFFTTMLHADEGMWLLSTLKQLNEADMQKMG
                     FKLTADDIYNINKSSMKDGIIHFGGGCTGEIVSPDGLILTNHHCGLDYIQSLSSIDHD
                     YLTNGFWATARDKELAATGLSVKFLIRMEDVTQKVKAELSDSLSEADLQAKLPGIFSK
                     LTKEATAGNDFYTADVRAFYGGNEYYLFVYERYEDVRLVGTPPMSIGNFGGDTDNWMW
                     PRQTGDFSMFRIYTSPDGKPAKYAKENVPLKSKYYFPVSIKGVKEGDFAMIMGYPGRT
                     DRYATSYSIENGLDITSPSLVKLRTKRLDVINNAMADNDAIRIKYQAKRNQVSNYWKY
                     FIGQQKQLKRMKVVEQKRNLENQFTTWVNADKKRRLHYGFVMGNLQQSYDNARKYAKL
                     RVYTSEALMGTEILRYAFSFSALEKALSEKEPNADDIKAKAAMLKSRVEDYFEEYDAG
                     VDQKVFAVLVKSFADDVAKDQQASIFAEMAKKYKGDYNKMAAAVFAKSIFSDKNKVLA
                     FLEDPSAKKLKNDPAFKLISTQLNFYDDNFRAPIRTIDLALAANNRLYIEGLREMMPE
                     KKLYPDANSTMRLSYGSVRAYDPADAVKYSYFTTIEGVMQKMDNSSTEFKVPEKLVEL
                     YNKKDYGRYGSNGTLNTCFLTNNDITGGNSGSPVINGNGEIIGLAFDGNWEAMSGDIK
                     YDEEYKRTICVDIRYVLFCIDKFGGAKHIVDEMKVVE"
     gene            19857..20327
                     /locus_tag="PKY09_04120"
     CDS             19857..20327
                     /locus_tag="PKY09_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12073.1"
                     /translation="MNKLKMFFIAAAITFVLSGCGTKQSNNSSVEKVNKAVNDEPVQY
                     DPDETIELDNGQKWKVNEEMKPFLLKGKEILNEYIQNEDNDYTGLAAALSEQDNKLIE
                     SCTMEGKSHEELHKWLHPHLDIISDLESTKDTAVAKKLVEQLKASYVDFEKYFQ"
     gene            20489..22645
                     /locus_tag="PKY09_04125"
     CDS             20489..22645
                     /locus_tag="PKY09_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNB12074.1"
                     /translation="MTEFFNQLLHEIERPLTNPVLIFSLILFIILLSPILLRRLNIPG
                     IIGLIIAGVIIGPHGLYILEKNSAINLFSTIGLLYIMFIAGLELDLNEFKSHRNKSLV
                     FGFFTFIIPLAIGFPVCHYLLHYDFNASFLTASMFATHTLVAYPIVSKLGVSKNQAVA
                     ITVGGTILTDTAVLIMLAVIMGNSQGNLNQEFWIRLGASLIIFSAIMFIVIPRIAKWF
                     FRKLESEKHSHYIFVLSVVFFAAFLAEVAGVEPIIGAFVAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVSVIMNGPTALIIAGTLTIVAITGKWLAAFLTQVIFK
                     YSSSQRQLIFGLSSAHAAATLAVILVGFKAGILDENILNGTIILILITCVIASFATER
                     AAKKIIVETEDDSSEILNNSSASNEHILLAVANVNKMEKLLDFAILIKDKKSVNPVSV
                     LSVVSDNSEAEKNIIKAKNKLEDFVKQASGTETKVNVITTIDHNASSGITRCSREIMA
                     DIIVLGWPQRTGFLDKLIGEKIESILNNTDKTTLICFLEKPLELHKRIVVAIPPLAEH
                     ENGFGLWLTKLAKLAQELSIPIHGYCNEVTEKSIRRYFNKEKISASLHISLFSDWDDF
                     LVLARNIHADDFVVLVSARRGATSYMSILDGLPLKIEKHFSVNSRLIIYPQQYDGNTN
                     LENYSEVSAETLNRGIEVVQKIGKEIGSMLKKKNQE"
     gene            complement(22662..23168)
                     /locus_tag="PKY09_04130"
     CDS             complement(22662..23168)
                     /locus_tag="PKY09_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HNB12075.1"
                     /translation="MEQEIKNRVADSGIIQIDPKDFIPATTIIKLDIKGWLFEEMILK
                     EKDFREHIKQHNWQQYNGAAVAVYCSVDAIIPTWAYMLISEALQQHASTIFFGNTTQL
                     KEKLAIDAIEKINIENYSNKRIVIKGCGDENFSPAVYMLLTQKLKPVVKSIMYGEPCS
                     TVPIYKSK"
     gene            complement(23171..23485)
                     /locus_tag="PKY09_04135"
     CDS             complement(23171..23485)
                     /locus_tag="PKY09_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNB12076.1"
                     /translation="MEEPKELYDRVIETIKTCYDPEIPVDIYELGLIYEVKIIEGNDV
                     HVKMTLTSPACPVAESLPLEVEQKIKSIPDVNDAKVELTFDPPWDKDMMSEEARMELG
                     MW"
     gene            23653..24057
                     /locus_tag="PKY09_04140"
     CDS             23653..24057
                     /locus_tag="PKY09_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNB12077.1"
                     /translation="MKQFLVIILTVGLSLSSSAQQKTVLEANEKAAIISFEKTEHNFG
                     KIKEGDKAEHVFLFKNTGNTPLIVTDVSVTCGCTAAEKPDAPIMPGKDGKIKIVFNSA
                     GKVGSQNRTITVSTNAKNKITYLKMTGEVSEK"
     gene            complement(24073..25162)
                     /gene="prfB"
                     /locus_tag="PKY09_04145"
     CDS             complement(join(24073..25101,25103..25162))
                     /gene="prfB"
                     /locus_tag="PKY09_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790626.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNB12078.1"
                     /translation="MTQEQLTECKERVDALRGHLDIDVKLLKIKEEEQLTHAPDFWNN
                     PTRAEQILKSIKLKKNWTDTFAAVQQAVDDLGVLFDFSKTGDASEQEVEEQYTSVQKS
                     LEELEFKNMLSGEEDALSAVLTINAGAGGTESCDWASMLMRMYIRWAERMNYRINEID
                     RQDGDVAGIKSVTLEIEGDFAYGYLKSENGVHRLVRISPFDSNAKRHTSFASVFAYPL
                     IDDTIEIEIRDADIEMQTSRSGGAGGQNVNKVETKVQLKHLPTGIVVVCQVERSQSGN
                     RERAMQMLRSQLYELELRKRNEAKAATEAGKKKIEWGSQIRSYVFHPYKMVKDLRTDC
                     ETSNVQAVMDGELHEFMKAFLMEFGERV"
     gene            complement(25207..25581)
                     /locus_tag="PKY09_04150"
     CDS             complement(25207..25581)
                     /locus_tag="PKY09_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12079.1"
                     /translation="MNKGFITYILQLAGITLLYILLYYLMKDFLAARFYPSWWKCGIF
                     FFILFSVIHYLRLAKSKGNESIRFFMASTAFKLFLMLTVIVSYMFLNPDTAIPFAVSF
                     LMAYFLFMIFDVAKAYKLMKTT"
     gene            complement(25578..25811)
                     /locus_tag="PKY09_04155"
     CDS             complement(25578..25811)
                     /locus_tag="PKY09_04155"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNB12080.1"
                     /translation="MSKDQKPNSRKNQLNDYVRYASMGFQMGAIMFIGTYGGYRLDKY
                     CQNKIPLFTVTFALIAVAAALYLTLKDFLHKKK"
     gene            complement(25768..26199)
                     /locus_tag="PKY09_04160"
     CDS             complement(25768..26199)
                     /locus_tag="PKY09_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNB12081.1"
                     /translation="MFENLKNKTTTVKSGETTSGSINLIAAGTSIEGEIKSNGDLRID
                     GHIVGSVFSKAKVVVGTTGVVDGDIVCQNADVSGTVKGTSTVTEMLFLKATAKINGDI
                     NTGKLVVEVGASFTGNCNMGPMVKDIKNVERSEAKLQEKSA"
     gene            complement(26265..28940)
                     /locus_tag="PKY09_04165"
     CDS             complement(26265..28940)
                     /locus_tag="PKY09_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12082.1"
                     /translation="MKSSNHLKYFITLTLFLSACSTQKNTVVNRAYHNLTAHYNGYFN
                     ARERVKEGTKTLASLHVDHYDRVLDVFKYATDDKAKTIYPDMDEAIKKSSIVIQRHSM
                     FINGKERCRWIPDNYMVIGKAQFLKHDFWTALETFQYVASTYKDNPKRFDGLIWQMQT
                     QMQLGKMADAEYMLDYLKNEKQLSSKYKPLFAAVAADFYLKKENYPKAIEQLNKAAEL
                     ATKRADKIRYNYILAQIYQKVDSNEKAFAIYRKVIKMNPEYEMAFNAKINRARSFDVN
                     SADAQQVKDELNKMLKDEKNKDYLDQIYYALSGISMREKDTTEAFQFLKKSVASSTNN
                     ANQKGISYLEMGEISFRKLDFRAAQLYYDSAATNITSDYPDYALIQNKKNNLSRLIKN
                     LNIISHEDSLLTLGALSETEQYSRIDDIIAAEEAESLRKKQEEEAAKAKEQEEQSNMQ
                     FSQFQNQPGKDGMPGNSTGSSWYFYNQSAISFGFSEFAKKWGNRKLEDNWRRSSKESI
                     ATFGSEEEAEAQARADSLEQAQKALNDSIRQLNSEARRKAYFNNIPKTTQQKEESNAK
                     ILEAYYNAGIIYKEALGQPKPAADDMEEMMKRYPNNKYELPAYYNLYRCYLALFDEPK
                     AEYYKKIILNDYGDSEYARIITNPNYFKDAQRKTAIMQVFYENTYKAFLNRQYTDVIE
                     RKSAADSLFPGGTLAPKFAYLQALSIGYTQPRPQFEASLKKIVAAYPRDSVSILAKAI
                     LDKMNNVAPIDTTISESATSQTAEGNKMLNSIYEFMPDKPHNVLIIFSAELPYGTTLK
                     EKINKFNQRNYKEKSLNVTELRVDDKNYVMVRSFPDHTQSKEYIQGLDSDQTVFEGMD
                     KSAFTLFPITEKNFNKFITDKDLSKYLDFYRVNYP"
     gene            29064..29942
                     /locus_tag="PKY09_04170"
     CDS             29064..29942
                     /locus_tag="PKY09_04170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB12083.1"
                     /translation="MAKASGKEKVKLSTKLRNKFRLVIMNDETLEEKASLLLSPINVV
                     VVVGIAIITLITATTYIIAFTPLREYIPGYTDVKVKRAAIESLIRTDSLQKQVSAQDL
                     YIQNLQNLITGQSQKTVSLDTVSVPQLYDTIKQLLHSEEDSLLRSQIESPQDYNLTLN
                     AGTTKGSIANFYFFAPLKGAITTRFNPTEKHFGVDVVAGPDAVIKSAMDGTVVIAEWT
                     SQAGYVIAIQHSNNMFSVYKHNSALLKKVGNVVKAGEVIAIVGNSGELSNGPHLHFEL
                     WYNGAPVDPTDYIAFE"
     gene            complement(29943..30995)
                     /gene="mutY"
                     /locus_tag="PKY09_04175"
     CDS             complement(29943..30995)
                     /gene="mutY"
                     /locus_tag="PKY09_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNB12084.1"
                     /translation="MNISNKLILWYERNKRELPFRSTKDAYRIWLSEVILQQTRVEQG
                     LPYYYRFLESFPTVKHLAKASETEVLKLWQGLGYYSRGRNLLATAQHITKILHNKFPS
                     NYLELKKLKGVGDYTAAAIASMAFNEPVFTVDGNVTRVLSRLFAEKTTPNSSVGKKVF
                     QQLAENIGNKKFPGLHNQAMMELGALVCTPRNPKCEICPLQSHCLAYQQKQQLNFPVK
                     SKKIKIKVRHFCFIILLYRNKFYIRQRNGNDIWKNLYEFPLLETQTKISSASISKFAK
                     NYGVTKYESGRTHIHKLTHQHIHASYIVAHLSKPISPKKFPELKAVDAEDFKNNFPHP
                     RLIEKILSENNYFDLL"
     gene            31099..31941
                     /gene="panC"
                     /locus_tag="PKY09_04180"
     CDS             31099..31941
                     /gene="panC"
                     /locus_tag="PKY09_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNB12085.1"
                     /translation="MKLIKDIHSLSQIVSSKKHEHINIGFVPTMGALHEGHISLIKQS
                     LLENDFTIASIFVNPLQFNNPDDLKKYPRALDSDMQMLESAGCNALFAPETSEVYSGD
                     EQPLQFDLGNLANIMEGKFRPGHFAGVITIVHKLFSWVKPDVAYFGEKDFQQLAVIRK
                     MAKALHPNVIITGCPTLREPNGLAMSSRNVRLNKTVRHNASIIYKAMQHARQLKNVLP
                     VYEVCSKVRSMIEEVAPFKVEYIEIADAVNLQPLQQWSDTQSARIFVAVFANDVRLID
                     NAAL"
     gene            complement(31947..32822)
                     /locus_tag="PKY09_04185"
     CDS             complement(31947..32822)
                     /locus_tag="PKY09_04185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010603248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB12086.1"
                     /translation="MELLTPQNFKDYELLDSGNFEKLERFGQYITIRPEPQAVWNRQK
                     KEADWLKEAHVKFVAKNSSSGTWKKLKEIPDRWHISYALPDAKQIRLRLALTSFKHVG
                     VFPEQAVNWDFIYHSVKKLDVVQPKVLNLFAYTGAATLAAKVAGADIIHLDSIKQVIT
                     WAKENMTLSGLDNIRWLVEDAMTFVKREVKRGKKYQGIILDPPAYGHGPNGENWKLED
                     EINEMLANVKLLLEENHFLVLNTYSLGFSSIILDNLLDEHFSKNIKRQTGEIFLQSTA
                     GARLPLGVFARHCTV"
     gene            32911..34050
                     /locus_tag="PKY09_04190"
     CDS             32911..34050
                     /locus_tag="PKY09_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB12087.1"
                     /translation="MIINTSELSGRSIQATDKAPLKILLVDDIPANLLTLEGILEDES
                     RILIKAFSGNEALKLLMEEPIDLILLDIQMPDMDGVEVAQLLRANPKTKHIPIIFVSA
                     IAKSERPSLEEFETGSFDFIPKPLDIEETQKIVSIFEFGCRYKLKYREQEEKLKRLND
                     HLDKFLYILSHDLNAPLRAIENLVQWLEEDLQSQISDESKENIGLLRNRVAKMKLLLD
                     GLLRYARSGNTKHHQDMISPEKMIRQIVAELQGDRKISLNIDSSVLEMQTDRTALQLV
                     LKELIQNAIFFNPSDKSTIDISISEKNDSYEFVVADNGPGIKPQHQKIIFELFQTLPA
                     SVVPSSGVGLSIVKKVVEEYGGTVWFSPTVKCGTEVHFNWKKSKI"
     gene            complement(34047..36440)
                     /locus_tag="PKY09_04195"
     CDS             complement(34047..36440)
                     /locus_tag="PKY09_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNB12088.1"
                     /translation="MSNTDTVNKVKCYHCGDDCDEQVITSDEHTFCCTGCKLVYEILR
                     ENNLCNYYNLNNNPGIKQSDILQKRLAFLDDPAIENQLISFRDKNETIITFYIPAMHC
                     SSCIWLLENFQKVDEGVLSSRVDFVRKELRLVYNHHKTSLRKIAEKLSQTGYEPALQL
                     DAIEKKKTKKANRSRIIRIGVAGFCSGNIMMLSFPEYFSSGNNIDASLQTLFSYLILI
                     LALPVFFYSANEFFIKSWQALKQKSSSIDLPIAIGITVIFLRSAYEIITQTGSGFFDS
                     GSGLVFFMLIGRWFQDFTFDSLAFDRDFKSYFPIAVTSIKNGHEKEVQINDLKVNDRI
                     LIRNNEIIPADCILLKGNASVDYHFVTGESLPVSHAAGEYIYAGGKQAGAMIELQVMK
                     EVSQSYLVQLWNSNWNKQDISRFEKLVNAISRWFIVVTFSISTAAAIYWWNTDVHKAI
                     NAFTAVLVIACPCALALSAPFTYGNILRILGRNKIYMRNYRVLEKIADADTIVFDKTG
                     TLTKNNVSEISYHGHPLDDVDKTMLLSLFRQSSHPLSKLLQQHLQANKFYPCNNFVEV
                     AGKGVSAVINNKNIKAGTVDWAEVNNENEENATAVFINIDNFKKGKFLFSNIYRDGLN
                     TISHKLNHNHSLVVLTGDNESERNNLTSLLGNAELRFNQKPEDKRNYIIDLINSKKNV
                     LMIGDGLNDAGALLAANTGISVTDNTNNFTPASDTIMDATALRQLPELINLSKSAVTI
                     IIISFIISLLYNFVGLAFAVSGTLSPLFAAILMPISTISLVLFTVGASTIKARLSGF"
     gene            36576..37952
                     /locus_tag="PKY09_04200"
     CDS             36576..37952
                     /locus_tag="PKY09_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB12089.1"
                     /translation="MQIKQFYNQGHSQFSYIISSGKEAVIIDPVRDIEHYLSYLKDKE
                     LTLKYIFETHIHADFVSGHLELADKTGATIVYGPQVRVAYPSYSARHNEGFNLEGCSI
                     KVLHTPGHTMESVCYLLNNSIGQPIAIFTGDTLFVGDVGRPDLMSGNKNAVELSAYLY
                     HSINDIIKSLPDELVVYPGHGAGSFCGKNPGKEKFTTIGEQKKRNYALKDISLEKFTN
                     EITSGLSVPPEYFFKVMSINIMGCRPMKEVFRNNLNFLSPVNFKAETQRALIVDTRNE
                     LEFSKAFIKGSVNIGLDGSFATWAGILLDPDHPVLLVCDNGREKEAIGALAQIGIENV
                     RGILQGGINAWKDIGEPVDSVENIDAEDFYFLFENEGYILVDLRSHQNFKEQHIKRSL
                     SIPLDELPHRLNEFNSDTKYLLVCKGGYHSMMAASILKRNGINQVVNISGGFDAVSAF
                     QPQYVESV"
     gene            38243..39064
                     /locus_tag="PKY09_04205"
     CDS             38243..39064
                     /locus_tag="PKY09_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB12090.1"
                     /translation="MEKIVVTTDFSECSENALIYSLSLCKELKASLYLLHVFHIPVPV
                     TDMPYISVSSEEEEKNIKLRLEELITRVRHKTGYEGMIHTITDFNFTVDGILNVVHDT
                     NADLLVMGQRGKTTLADRLFGSTATAIINLVSCPLLLIPEGCSFRKLKKIAIACDRKT
                     GDNKNKVFHVAEMIATLKSEILLFNVVQDETEKDKLRRDDFSEMAGNDVKLFFPVHHD
                     IHEGILQFVSDNDADWLCMFPHRHNIFEQWFTRLNSGNISQQVKIPLLLINELVH"
     gene            39143..39556
                     /locus_tag="PKY09_04210"
     CDS             39143..39556
                     /locus_tag="PKY09_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12091.1"
                     /translation="MKHRLTIQAEDTILDFQKKFNRIYPFLKIEFFEKAHIAKQGSHK
                     REMITSNRLFHGLIGRGKTHIINFNDNFSISDFEMLLKNQCRLYAQVFRKSGKIWLET
                     TATDYWTLEQQNEEGKSLAKHFKIESENINDIEPY"
BASE COUNT        12195 a         7177 c         7638 g        12580 t
ORIGIN      
        1 cagaaacaga agatgcagca tttacagcaa tagtttttga tttagagcaa ccattggcag
       61 tttcaataac tctgtacgac ccattagatg ttgctgtata attctgagaa gttgctccaa
      121 gaatatctac actgtttttc tgccactgat attgtacacc ggtaaaacta tttgctgtta
      181 gtacaacaga acccggacta caaaaggaag taggtgtagt atgagaaata gaaggtgtag
      241 ggttgttaat ctgattacct atcacgctgt ttgttgttac agtacaagac cccgatgtaa
      301 tgacacaata ataggttcct gatgaaccaa tattaataga agaatttgta cttgaaagca
      361 gcacattgtt tctgtaccat tgataagtaa atcccggacc ggaagtagca tttaatgtgg
      421 cattagagcc tgcacaaaaa cttactgttc ctgatggggt aatttgccct gtcatagcac
      481 caatgcttac gctaactatt gcagaagtac tgctgcaaag gtttgaattt gtaactacta
      541 cagcgtagtt gcctgttgca gtagcactat aagttggatt gtcagcacct gaaatcacat
      601 taccattatt gagccattga tatgttaatc ctgtacctgt atttgcagaa agcactactg
      661 gtcctgcaca aacattaatg gtaccggaag gtgaaatcac tgctgttggc gtatcacata
      721 aagtggtaaa aggaacagta ctactccatg gagaaacggc taatccacaa accagttgta
      781 cctgccattg ataattagtg ttgggttgta aaccggttaa cgtatatgga gtagatacat
      841 tatttaaaat tgtccatgtt gaattgcttg ctttcttata ctgaaggttt aatgcactac
      901 ctgcagaacc tgaagaatat aaaactgccg aagtactgct tatagaagtg gtataattgt
      961 tttgtggtgt attacatggt tgtgctgttg tagagaaagg tgttgatgca gaaaaacctg
     1021 atgttgttcc acctgcacac actgtctcca cctgccattc gtaattgctg ctggctgtta
     1081 atgaataaac agaataactg tttgctgtag tattggccgt agtccatgta gaagctccaa
     1141 ctaaacggta acgaacatta taactaaccg caccacctac tgcagtccat gtataggttg
     1201 cactggtagc atttatgttg gtagttacag tgcctgatgg tacattgcaa gtagttggtg
     1261 gtgtaacaaa tgtggttgag atagtataga ttgatgttcc tccggaacaa actgtctgca
     1321 cctgccactc gtagttgctg cccggtgtca gtcctgtagc agtgtaaggt gatgcagatg
     1381 ctgttgcagt agtccacgtt gaagtaccaa caacacgata acgaacaaca tagcttgaag
     1441 cacttgctgc tgctgtccag ttaaaaattg cagatgtgct ggtgatggat gaggtattca
     1501 ttcccggagg aggcatacat gccattccaa tacaggcagt caaacatgca ccactgttat
     1561 atttattaat gattacagct tgtggctgcg aaccaaatcc taaagaaaga ttgataccta
     1621 cggaattcaa atgacaatag ctcataatag ttccaccacc ggaaggaatg ggtgcgccac
     1681 tacaacttcc ttcataacca taacctgcag caggaccaca gccatcaata gcagtgttgt
     1741 taccattcca cacacaggca tgtgtatgtc ttgaccccat taagtgacct tgttcgtgag
     1801 taacaaccat aacactccat gaatatgtcg ggaaattgct gtaagaggag ttgatgccac
     1861 tataacattt actggaattg atgtttgaag cacaaatgcc actgaaaccg gctgctatgc
     1921 caccaccacc accataacca agcaggtgac ctaaatcacc attaaagctt gtacggtatg
     1981 tctggaattg actcagatag tttgaagagg ttgttcctgt atatggactc acaacatccc
     2041 aaacataaac ttcactcaat tcaacaggaa tattgtcatt ggtataaata attgcactct
     2101 gattaaaaat tcctgtcaca tagtttgcag cagcagtgat gtttccttta ccttgaaaaa
     2161 tattataatt tacttcccaa tacaatctga tacagtttac catcatacct ctgttggcag
     2221 gcaactgtaa ttcctgtggt gtataagttc cattgtcggc aagtgttgca cattccattg
     2281 caggtcgtga ggtcaggtct gcatcgttat aaattacatg taagttagaa cgattgtttt
     2341 ctaaacggcc aatcacaaaa tttccgtcag ccggtgtaga aatcaaagcc atcacttcat
     2401 catcaaaaat gctgatggct gctatggaat tgttatcacc attaataata ccctgataat
     2461 gtaaaccggg agtatagttc acagccccgc cattgctgcc tgccgaagta acactaaaat
     2521 caggagtaaa aatgtttgct ttatacaaat caacctgcac cacattgttg ttaataggaa
     2581 tacgcagcga taaataatca ggctgctgac tcattaaagt tgcaattgcc gacttattca
     2641 actcaacaaa agttgcttta gtggcagtag gtgcatattg tctgttcagt tgttcattgt
     2701 ccggactaat agtaaaaaga ttctctgttg caaacactgc ctgctgttgc ttggcagtaa
     2761 atattttttg tgctacaggt ttaagattct gtgcccacaa catcatgggc gtaaaaatca
     2821 ataataaaaa gtaaaatcgt ttgttcataa atttgaggat tataagattc caaatatatt
     2881 gtgtttttct taatttcaaa atatatttgt aataaatgtt gctgtcagat gaacactcgt
     2941 attaaaagag gcatactgat aatctgtctg ctgttatcaa aatcactttt tgcacagaat
     3001 ctatatttcc caccattaac aggaaataat tgggatacac tttcaccatc agctttaaac
     3061 tggtgccagc cacgcattga ctcattgtat cagtttcttg aggacaggca tacaaaaagt
     3121 tttatcattc ttaaagacgg aaaaattatt ctcgaaaaat atttcggcac ctacactgca
     3181 gactccattc actattgggc atcggcagga aagagcctca ctgcttttct tacaggcatt
     3241 gcacaggaaa aaggtttcgt aaacattcag tcacccgttt cacaatatct cggcacagga
     3301 tggacaagtg caccacctgc caaagaaaat cttatcacac tacggcattt actaactatg
     3361 acaagcgggt tagatgattc gccaacacca ccatgcgatg taaatgattc atcaaagaca
     3421 tgccttaact accttgccga tgcaggtaca cgttgggcct atcattccgg tgcatataaa
     3481 catctgcaag acgtaatcag cgtaacaagt ggtatgtctt ataatgcttt taccacaaat
     3541 tatttgagct ctaaaattgg catgggcgga ctttggtacg atgcagtttt ttatagtaag
     3601 accagaggca tggcacgttt tggtttactt acattgagta aaggaatctg gaataacgat
     3661 accatcttaa aggacacatc atatattgct gccatgagca acacttcgca gaatttaaac
     3721 aactcctatg gttatttgtg gtggctcaac ggcaaggcat cgtttatgat accgcagttg
     3781 caggtagtct ttcccggagc gatgttgcct gctgctccac cagatttgtt tgctgctctg
     3841 ggaaagaatg atcagaaaat ttatgtcgta ccttcagaga atttagttgt tgttcgcatg
     3901 ggtgactctg cctacagcag tcagtatgca gttactgttt ttgataatga actgtggacc
     3961 tatattgaca gtttatcatt ctgcccgaca gcactcaacg aaatcaatac aaaccagcat
     4021 acatggattt atcccaatcc ggcaaacaat tttttacaat tagattcaga aacatccacc
     4081 tattttattt acaacattca aggccggtgt tgtttgcaag gcaagcctga tgcacaaaga
     4141 aaaattgata tttcaatgct tcaagaagga gtttattatt taaaaataca gactaagaat
     4201 aatcagcagt ggtattttaa atttatcaga aaagaataaa aactacaatt gccataggcc
     4261 tgttttgcga agatgatttt ccaaaacatc taagcgtgcg cttcccgtaa cacccatcag
     4321 gtctttaagc tgcgaaagtt ttagcttcca catttttgat aaagcaatca actctttttt
     4381 gttgaaggga ttgatcgttt tcttattaat gaaatgtttc atattacaaa tacattatgc
     4441 agtttgtact gtaaaaatta tgccaaggtt acaacagcac actgttatgg ggttattcat
     4501 tttggtttca tcaaggctgc atcaaagcta aacgcataag atgaatagtt gaaggctgcc
     4561 atcctacaat ctttaactgt gaggaagttt aaaaattgat aacatataag ttaaatctgt
     4621 aaaatttata tccaaaagca gattaatttt cacaagcatt attttattga cttttatatt
     4681 gaacagccgg ggaaagtgca gaaaaaaaaa tgaaaattaa ttagccatat atttattttg
     4741 aaaaaggaat gtattcaaac aatttttatg attaaagaca acactttatt aataccggat
     4801 tacttcaatt tttaagatta aaaatattag tccaagaaat ttttaagcaa tggtttttta
     4861 taagcaatcc cactacttac tattcgcaac atcttctttt ccggctttta ctatttcgcc
     4921 atattgcgtt tcgtcaccat caaaagattt tagttttatg cggtagtagt ttgttgtacc
     4981 tgcatcagtt ctgtcaacaa aagaatagta gtttatctga ttgttacggt tggcttttac
     5041 caatccggct tcactaaagt cggttccgtt agcagagcgc tctacaataa aatagttgtt
     5101 gctatcatta gttttgcatt tccacaccac actcgatgtg tttgcatgtt gaataacttt
     5161 taattcaggg tcatcatctt ttgatattgc agtaatagca acggcaccct ctgtcaacct
     5221 ccatgcacgg tcaaccccca acgattttag aacaatattt actttcagta cgggtgattt
     5281 ttcaccttca acttcgtatt taaatttttt gctttctgtc atatcgctgc ctttaacaac
     5341 aaacgatttt gcaatagctt cattttgcat aatataaata atacactgat catcggaatt
     5401 gagcttacca tctgcagtta aagttacctc tacctgatag cgatagtttc ctttttcatt
     5461 acacaatctg ttatttgaag tgaaaatggc agagtcgttg cgcacttccc aatattcaga
     5521 gttaaaaaca ccaccggaaa ctttaaaggt ttcacttttt ttaaagttta ccgaataaat
     5581 aactttatca cccgccagca gacaggttgt tagtgtcatt aacagcagtg ttccaatagt
     5641 ctttttcatt ttcatgagtc cgattttttc tttatacaaa tattgaagaa taagggtgta
     5701 tgtttatgct acttttgcca tacttttcaa atggcaggtg tttatatcca tattccgttt
     5761 tgcaggaagg cttgtaccta ctgcgacttt catttttcga ctcagctaca aaacaagaac
     5821 gcatttgttg aagccttgtt gcatgagata gaactgcaga cacattatat taatgaacct
     5881 gttacaacaa tatatttcgg tggaggcaca ccatcgctac tcacagcagc agagataaac
     5941 accattatcg aaaagttaaa cactgtgttt aaacttgatg ttaaagaaat tactttagaa
     6001 gccaatcctg ataatctgac aactgtatat ttgaaagagt tgcagacaac agcagtaaac
     6061 agattgagta ttggtgttca gtcgttcgaa gagcagcgtc tgcaatggat gaacagaagt
     6121 catactacac agcagtcagt tgtagctatt gagtctgctc tgcaaattgg atttaatgta
     6181 agtgccgatt tgatttttgc cttgcctgaa atgtcaaatg atgaatggca aaagcagcta
     6241 cagaagatga ttggtttaaa accgcatcat gtttctgcct atagtttaac actggaagaa
     6301 aggacggttt atcatcatca ggttgcgaag aagaaaatta aggagctaaa cgatgttcaa
     6361 tcagaagcgc aatttatgct ggcacatcag ttgttgagcg aggcaggata tcatcactac
     6421 gaaatatcga actacgcaag tgatggacac gttgccgtgc acaacagcag ttactggaag
     6481 gcagaaatat atttaggtct tggcccatcg gcacatagtt ttaacggaaa aacgcgccaa
     6541 tggaatatca gaaataacac agcttatatt aaatcattgc atgagggaaa acttcctttt
     6601 gaaagcgaaa tgctgacaga agttgacagt tataatgaat atgtaatgac acgcctgaga
     6661 accgacaaag gaattgacga aaaacagtgg cataaattgt tgcctgaaaa tcataaggtg
     6721 aatttactgg cggaaatgaa tgagttgaca ggtcagaaat ttttagtacg tgacgcttct
     6781 cgtatattta tccccttgca acattggtac aaagccgatc agataatcag tagattgttt
     6841 gcaacagaaa ttagctaaaa gaaatgattg tacatttaaa aataggaaac caagagtatt
     6901 cagcaaagct caatgagcca atagatattt ctattccatt gcactccggc aatgataatg
     6961 ttaatgcatg gtatgctcct ccaccggaaa ttgttcctgt gcgcatggat agttgggtag
     7021 gagaagtagc acagggtgca gcagtaaatt tcagaaatgt gttttttaat ccgcatgctc
     7081 atggcacgca tacagagtgt gtaggacata tcaccaaaga gtgggtttca ttaaataaag
     7141 tattgaaaca gtttttcttt tcagcaaaac tgattacggt actacctaag attgccgatg
     7201 gcgataggat tatcaccaag gaagatatag tgcaggcatg ggaaaactgt ggtgcagaag
     7261 cattagtcat ccgtacccta cctaatgaca acagcaaaca atgcagacat tattcaaata
     7321 ccaatccgcc ttatctacat catgaagcag cagcgtggat ggcagaacaa aatatcaaac
     7381 atctgttact tgaccttcct tcggttgacc gtgagcagga tggagggaaa ctactagccc
     7441 acaatgcatt ctggaatacg tcaaaaaatg ttcgttatgg ttgtactatt tcagaaatga
     7501 tttttgtgcc cgaccatatt gccgatggac tatatttgct caatattcag attactgcat
     7561 tggagaatga tgcaagccct tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nncttgaacg tatgctggag cgaacaccgt tgttaaaaat tatttacact tcagtaaaag
     7741 atctgataga agcctttgtg ggagagaaaa aacgatttaa ccagcctgta ttggttacgg
     7801 ttgataaggt gtcgggagta cagcgcattg gtttcatcac acaaaaagat ttatcggtgc
     7861 ttggcatcag cagtaataaa atggcagttt atcttccttt ctcctatggc tttaacggac
     7921 agctggtgat agtacctgca gagaatgttg agaagataga tgcatcaggc acagagatga
     7981 tgaagtttgt aatttcagga ggtgttacag aagtagatta aacaaggagc tatgaatatt
     8041 tttcggtttg cattatttgc gacaatgtta attttaatag ggtcaggtgt ttgtgcacag
     8101 gattatttta ttccgctaaa cagagaatgg aacctgcgtt atgaaccctt tcttcagact
     8161 acaaagagca ataaactaca tacatccatc aggccttatc gtaatgatga aattattgct
     8221 gttgcaaaca ttgattctat tgacacggct aattttccga taactcgttt ttcaaaaacc
     8281 ttggtcggtc gaaaaatttt taaagaacat ttagtacaaa tcaaggatga tgattttctg
     8341 attcatgccg attttatttt tgaaggcaga ataggtaaag accttcccga aacagaaaca
     8401 ttgttcacca attcaagagg attttggctt ggcggaacgg cagggaaaag attttctttc
     8461 tctacaacat ttttcgaaaa tcaggcaagg tttcctgctt acattgatac gtttgttaca
     8521 cactacagta tcattcccgg tgaggggcgc ataaaaaaac agtatggcag ttatgattat
     8581 tccaatgtta caggaaccat atcatacacc atcaacagac attttaatgt acaatttggt
     8641 cacgataaaa attttattgg tgatggttat cgctctttgc ttttgtctga caatgccaac
     8701 agctatccat ttcttaaaat ttcaactacg gcatggaagg ttaagtatgt aaatctgttt
     8761 acagtactca ccgacatgca gtttcccgca cctaacgata ttgctttcaa aaagaaatat
     8821 gcatcattcc attatctaga ccttaacatc ggcaaacatg catctttcgg aattttagaa
     8881 agtgttgtct ggtcgggaga ctcggcacgc gggcaaggtt ttgatattaa ctatatgaat
     8941 ccttttgtat ttatccgacc tgttgaattt tcaataggtt cgccagataa tatgatgctg
     9001 ggttttaacc tgaaagtgaa actgttttcg aaattggtat tttatggaca gttgatgttg
     9061 gatgaattta aactttcaga aataagagat ggtaatggct ggtttgctaa caaacaagca
     9121 tttcagttag gattaaagag ttacgatttg ccaattaaaa atcttgatgt gctgacagag
     9181 tttaactatg taagaccttt tacctattcg caccgaacaa cctttacaaa ctatgcacat
     9241 ttcaatcagt cgctggcaca tcccttagga tcaaacttta ttgagtcggt atccttgatc
     9301 aactacaagt ataaaaattt tattttccgc tttcagttta actatgctga aacagcaagc
     9361 gatacaggta atgcaaattt gggtaatgac attttcaaga gctatgaaac ctacactttt
     9421 gcctatggca acagaatgct tcaggggcaa cgtcacaacc tgatttatag cgacatcaga
     9481 gcagggtatt tgattaatcc acgaacaaat ttatgttttg aaattggtta tagctttagg
     9541 agaatgacaa tggctaaaga agcagccact acaaactggg tttatgcagg cataaggaca
     9601 gcacttttta accgctatca tgatttttaa ttacgcctat atttaaagtt gttttacctg
     9661 aaaaaaacac gtgttggcaa aataatacct ttgtgctgat ttaacaaaga tacatttgag
     9721 ccaaacagat tacgcaccat gattaatatt gcttttagtt gtattaccgc ttttattatt
     9781 acttttcttg ccataccatc aattattaag gttgccgtca taaaaaacct ttttgatgag
     9841 cctggaggac gcaaggcaca taaagctagt gtaccaacat tgggtggctt agccattttt
     9901 gccggtgttg tttttgcatt tacattttgg tcagcagggt ttaatcttca ggacacacag
     9961 tacatcattg cggctttgat tgtgatgttt tttatcggaa ttaaagacga catagttgaa
    10021 cttcctgcaa tgaaaaaact ttatggacag atagtagcgg ctgttattat tgtcttgttt
    10081 gcaaacattc gcataacagg tctttatggt attttcggta ttgatcaagt gccatactgg
    10141 ttcagtcttt tgttttcaat gtttactatt ctgcttatca tcaatgcttt taacctgatt
    10201 gacggtgttg acggattggc agcaggtatt ggcgctattg ctgcatttac ctatggaatt
    10261 tacttctaca actacaatga gatagctctt tgtattcttt cgttttcact tttcagttcg
    10321 ctgttggctt tcctggttta taatttttct cctgccaata tttttatggg cgataccgga
    10381 tcgttaatta ttgggttgat tttatctatt ctcacaatca agtttattga aatgagtttt
    10441 gcaccacgtc ctgagaactt cccatttcag agtagtccgg ctatggctgt tgctattttg
    10501 gtgctgccat tgtttgattt gctcagagtt tttattgtca gaattttaaa caaacgttca
    10561 ccttttgagg ctgacagaca acatatccac caccttttgc tggatatggg attaaatcac
    10621 cgcgaggttt ccattacact ttacttggta aacataggct ttatacttgt ggcattggca
    10681 ttacgtagct ttggtgcatt ggcattatta ttcattatct ctactttagc cttgattttg
    10741 agcttggtgc cctactattt aaagtatcag gcaggccgct caggagccgt ttcgaggtcg
    10801 cataagtagg gcgttgatac cctaattgcc gcacttacat gaaaaattga tataattatg
    10861 taagcatttc ccaaaaaagt aaacattttt tttattttcg tccgaacttg aatgacaggc
    10921 atttttccgg actctctgcc tgcgaaaatt attatcatgc gactaaaaaa attggtttac
    10981 tccattgctc ttttattgcc gttcataggt aatgctcagg tagttgaaaa tacacaaccc
    11041 caatccggtg accctattgc tgccatgctc gacagtttag tgtctattcg taacgttgtg
    11101 cgactgagcg aaacaaacct tcaacagcct cctgttttta gtgaaaacga tgctgtactt
    11161 tctgatgctt ttattcagga gaggatttca aaaatttcta cacctatacc gttaacctat
    11221 aattatcagg taaaaaatta catctccctt tatgcttcac aacgcgcaca gttgacttcg
    11281 cgtgtacttg gtttgtcacg attatacttt ccttattttg aacagatgct ggacaagtac
    11341 aacctacctg ttgaattaaa atatctggct attgttgaat cggcacttaa cccaacagct
    11401 gtatcatcag caggtgcaac aggtatctgg cagtttatgt acaatacggg aaagctttat
    11461 ggactgaaca ttaactccta tatagatgaa agaagagacg tacgtctggc aacagatgca
    11521 gcatgccgct attttcagga tatgtatgct atctatggcg attggttgct tgtgattgca
    11581 tcttacaact gtgggccggg aaatgttaac cgtgcaatcg cccgtgctgg cggcagcaaa
    11641 aacttttggg agataagtcg ttttcttcct gcagaaacaa gaggatatgt tcctgctttt
    11701 attggtgcta cctatgtaat gtattatgct aaagattaca acatcacacc tacaacgcct
    11761 acattcacct attttgatgt tgacactgtt ggcgtgaaaa ataatatttc gtttcgtcag
    11821 ttgagtcaga cattaaacat accttatgat gttattgcct ttttaaatcc tgtttataaa
    11881 cgcaacatga ttccgggtga gggtaacaga ctttatctac ctacagaaaa aataagtttg
    11941 tatctggcaa acgaagaaag tctttatgca tcaagcggca ctgagttgca accatcatca
    12001 tttgctattg ctgatgatgt tggctatgtg caggtgaaac aacatgtaaa agttcgtaag
    12061 ggagaaaacc tttattcatt tgcaaaaaga aattactgct ctgcaaagca ggtgaaaaaa
    12121 tggaacaatt taagatctaa ttatctgaaa gccggacaga agttggttgt atatacaact
    12181 ataaaacaaa aagcgacacc ggttgagact acaactgaaa ccgcagcaaa tgataaaaca
    12241 gattcagcta aggtgaatac agcagtcaag gcagactcat cagcaaacac agaaaccggt
    12301 gctgttactt ctgagagcaa aacaacacaa gttaaccaga ctcaaagtgc tgcatcaaaa
    12361 tgtattattc atttagtgca accgggtgac acactttgga atattgcaaa acgatatgag
    12421 ggtgttactg ttgaactttt gaaagaggta aataatttta caccccaatc aacacttaaa
    12481 gtaggaagca aaataaaaat acctactgta ggctaatttt attttcgtgt tttattaatg
    12541 tttgtcgtct tcaatatgct gacggaattc gtttttaata tcctgcagtg caggagtgag
    12601 aaatgttgaa gtttttagaa cagggtcata gagtttggac tcttttttag tctgtgaatt
    12661 gataaagttg agatgtggtt caagtgattt gagtaaccac aggaacacac tcaacaccaa
    12721 agcatatttc agtaaaccaa aaacaccgcc aataatttta ttgaaaagat tgaggtcacc
    12781 ggctttgaga ataccttcga gcaggcgtgc ataaagaata aacactgcca taattatacc
    12841 gctaataata aaaaagccta cccatgcagg cagggcactg tctttgttta ctaattgaaa
    12901 cagccagcct tgcacaactc cggaaaattt gaatgcaaca tagagtcctg caatgagacc
    12961 gagtatcatg gcaatctcat aaataatacc gcgggtgaaa cctttataag caccccacaa
    13021 taagggcacg gctaaaaaca aatcgagaat attcatactg tggaatagta cgctcaaagt
    13081 aagcgatgcc gttgtaatct aataacagca tcagcagaaa ataccaaaca ataaattgtt
    13141 gaaaggtgtt gattaaacca atgtaatatc aaactgaact aagtcaataa actcctgaat
    13201 gcgttcgttg agttcatcac tatcaaggtt catgattctt tctgtgccga atttttctac
    13261 gcagaaggaa gccattgcag aaccgtagat gatggcacgt ttcatactgc tgaaagagat
    13321 gtcgcgggtt tttgcaagat agccaatgaa gccaccggca aaagtgtcgc cagcacctgt
    13381 ggggtcaaac acatcttcta gtggcagtgc aggagcaaaa aatacttcgt tgttgtggaa
    13441 gaggagtgcg ccatgttcgc cttttttgat gatgagatat tttggtccca tatttagaat
    13501 tttcttggca gcttttacca aagaatattc gttggtgagt tgtcgtgctt cagcatcatt
    13561 gatggtaagc acatcaatca ttgaaatgtt tttgagcaaa tcgtccattg ccatatccat
    13621 ccagaagttc atggtgtcca tgacgatgag ttttgggcgt ttgcgcaaac gctcaattac
    13681 ctgcaactgg atttttggca tcaggtttcc aagcatgagg aactcgcagt cctgatagct
    13741 gtcgggaata atggggtcga agttggcaag aacgttgagt tgtgtgtcta aggtgtcgcg
    13801 gctgttcatg tctgtatgat aacgaccact ccagaagaaa gatttttcgc cctcaattat
    13861 ttgcagccct tctgtgcgga tgttgtggtt gttaaaatcc tgaatggtgc tttgcggaaa
    13921 gtcatcgcca accactccaa caagatttat tttaggagca aagtaggatg ctgcaagcga
    13981 ggcataggtg gcagcaccgc caattatttt atcggttttt ccgaagggtg tttctattgc
    14041 atcaaaggca acggttccaa caacaagtaa tgacatgatg tgagtttgaa aattttgcgc
    14101 aaagctaata ctattatcgg tttttgtgat agtgttttac aaatactgct ttgtggcaat
    14161 tgcgataaca gcaacaacaa tttcttcatt aaaattataa acagattcaa ttatctttaa
    14221 accttaatct tttaatacat aatatcatgt tcgaacaatt attaaatctt gtaaaagaaa
    14281 atgccggaga ggctatcatt aacaatcagg caataccgaa tgaacagaac gatgcagcta
    14341 ttaatcaggc tacggaaggg attataggcg gattaaaatc gcaattggca tcaggcaaca
    14401 tcagtgaggt ggcacaactg ttaggcggac agaataatgc agcaacatca ggtgttacaa
    14461 atgccatttc atcgcaggta tcgcaggggc taatggagaa gtttggttta tcaggaaaca
    14521 gtgcaggttc tatagtgtcg tcactgatac ctgttgtgtt gagtaagctg gtttcgaaga
    14581 ctaatgatcc taatgataag agttttgatt tgaatagtat ttttaatcat ttgagtggag
    14641 gtaaaacgca gggcatggat ttgtcgagta tattgggttc tgtgacgggt ggtaatacaa
    14701 gcggaggcgg aataggaaat gtgcttggct ctgtgatggg cggagataaa aattctgcac
    14761 ttgggggatt gatggatatt tttaagaagt aggataaaaa atacctcagt atcaaaaatt
    14821 ataaatccct ttggttctat caaattgatt ttactgaagg gattttctat tactaaactt
    14881 ttaaagcttc aaggcaacaa ttcttaaatc aaactttcca actttaaaat attttatttc
    14941 gccacactca aacctttaac tgcaaacgtc caatggcaat ggtcattcta ctgatgcaaa
    15001 cgggttgatt tttttcgttt agaatttcta ttgtccagac ctgtgtcttt tttccaagat
    15061 gaaagggttt ggcagtgccg taaacgaatc cttcttttac gggtcgcacg tggttggcat
    15121 tgatttccat gccaacagca acatatgtgt cgtcttttaa tgttagaaat gcaccaatgc
    15181 ttgctaacga ttctgcaagt gcacaagagg caccgccatg taatataccc atgggttgac
    15241 aggtacggtg gtcaacagac atttttcctt taacagcatt gttgtcaatg gaagttattt
    15301 cgatgcccag gtgactaagc atggtattgc gttctaattt ttttacatct tgcaatgtat
    15361 agttgccaaa aatagaattc attatttgct ttttttagat ggaaaaactg tgcggtaaat
    15421 ttcatccaca aaaattttaa gtcttagaat atatattggt gatgataaat tgtcttttag
    15481 ctgtttgttg tttttaagca tgacgcggta gcccacgcca aagcctatgc caacccaacg
    15541 gatgagttta tagtgtcctg tgatggcggg ttcaaagagt atgatgttgc cttgcaaagc
    15601 tttgttgttt gctttttttc cgtcaggata ttcgtagtag cttgtgccga agccaaagtg
    15661 cataggtacg tttacttgcc atggaccgtt gcggtagagc acatattcaa tgcctgttgt
    15721 aaaataattg cttcgcactt cgccacgata atatttgttt ggtcttatct ggacactgtc
    15781 cacaacatcg gtaaacagtt tcatgtagcc catgctgaat tttacacgtt tgccaaaatc
    15841 caaacctgtt ttgaaaccaa tggcattggc accgcgactg cgaacgaagg agttgtagcc
    15901 atctaactga aaaaaaggtt ttggtctttg tttaagtaaa taggccaacg agtcgcgcag
    15961 ttgtgaatga acattggtgc tgcatgaaag cagtacaaac attgtaagca gcgtgtattt
    16021 tatttttttg gaagacattg aaccgacaaa acaaaaatat agatgtgtac tgttttattt
    16081 ctgaataatt aatttgctta ctaacaattg catttgaatt acgaaactga tgcaggtcag
    16141 tgtgtttaga tgtaaaagtg tgtttatctt ttataacttt gcacaaagcg taattctcgg
    16201 taatcaaaag aaagctgagt tttaaaaaaa gaagataatg aaagtagaga ttaaagttcc
    16261 aagtccgggt gaaagcatta cggaagtaca gattgcacgt tggataaaga aagatggtga
    16321 ttatgttgag cgcgatgaag agattgcaga aatagattcg gacaaggcaa cactatcgtt
    16381 ggttgcagaa gctgatggtg ttttgaaaac acttgttgcc gaaggtgaga atattacagt
    16441 tggctctgta gtttgtacga tagatacaga tgcaaagcct gttgcaaagg cagaacctat
    16501 aaaagaagaa aagcctaaag aagaaaaagc aaaagaagtt aagcctgctg ctactgctat
    16561 tccttcaaag gcagaaggct atgctgcagg cacaccgtct gttgctgcag ctaagatgat
    16621 gaaagaaaat aatgtaaagg gtgaaagtgt gcaggcaacg ggcaagggtg gacgtattac
    16681 aaaaggtgat gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaagctgt
    16801 ttgtgaggca ttaaaatctt tccctgcagt gaatgcacaa attgacggtg aagaaatttt
    16861 gtatcacaac tattgcgatg tgggtattgc agtgagtgca ccaaaaggat tgatggttcc
    16921 gattatccgc agtgctgaaa aattatcgct tgcacagata gaacaacaag taatagacct
    16981 tgccaccaaa gcccgcgaca gcaaacttac tattgatgaa atgacaggtg gaacttttac
    17041 cattactaat ggtggtgtgt ttggctctat gttgtcaaca ccaattatta atccgccaca
    17101 gagtgcaatt ttaggaatgc ataatattgt tgagcgtcct gttgcattga atggagaagt
    17161 agttattcgt cctgtaatgt atgttgcctt gtcatacgat catcgaataa ttgatggcag
    17221 agagtctgtt ggatttttag ttaaagtaaa gcagatgctt gaagagccgg caaaaatgtt
    17281 gtttgaaggc aacgacccaa ttaaagtatt actacatttg taaccacttt tcttttacca
    17341 ataaacaaaa agaggctgcc tgattcaggc agcctctgct attttattta gtgttgtagt
    17401 tactcaacaa ctttcatctc gtcaacaatg tgtttggctc caccaaattt gtcaatacaa
    17461 aacaatacgt atctgatgtc aacacagatg gtgcgtttgt attcttcgtc atatttaata
    17521 tcaccactca tagcttccca gttgccatcg aaagcaagtc cgatgatttc gccattgcca
    17581 ttgataaccg ggcttccgct gttgccacct gtaatgtcgt tgtttgtgag gaagcaggtg
    17641 tttaatgttc cattgctgcc ataacgacca taatcttttt tgttgtacag ctcaactaac
    17701 ttttcaggaa ctttaaactc tgtagaactg ttatccattt tctgcatgac accttcaatg
    17761 gtagtaaagt agctatactt aacagcatcg gcaggatcgt aggcacgcac agagccataa
    17821 cttaaacgca tggtgctgtt ggcatcggga taaagttttt tctcaggcat catttcgcgc
    17881 agaccttcaa tatacaatct gttgttggca gccaatgcta aatcaatggt gcgaattgga
    17941 gcacggaagt tatcatcata aaagttcaac tgtgtgctta tgagtttaaa tgcaggatcg
    18001 tttttaagtt tttttgcaga agggtcttct aagaacgcaa gtactttgtt tttgtcggag
    18061 aaaatactct ttgcaaaaac tgctgcagcc attttgttgt agtcgccttt atatttctta
    18121 gccatttctg caaagatgga tgcctgctga tctttggcaa cgtcatcggc aaacgattta
    18181 acaagaacag caaaaacttt ttggtcaaca ccagcatcat actcttcaaa ataatcttcc
    18241 actcttgatt taagcattgc tgcttttgct tttatatcat cggcattagg ttctttctca
    18301 ctaagagcct tttctaaagc agaaaacgaa aatgcatatc gtaaaatttc tgttcccatt
    18361 aatgcttctg aagtgtaaac acgcagtttg gcatattttc ttgcattgtc ataagattgc
    18421 tgaaggttgc ccatcacaaa tccataatgc agtcttcttt ttttatctgc attaacccaa
    18481 gtggtgaatt gattttcgag gttgcgtttt tgttcaacaa ctttcattct ttttaattgc
    18541 ttctgctgac cgataaaata cttccaatag ttgcttacct gattgcgctt tgcctgatat
    18601 tttattctta tagcatcatt atctgccatt gcattgttga tgacatctaa tcttttagtg
    18661 cgtagtttca ccaatgaagg acttgtaata tcaagaccat tttcaatact gtatgatgtt
    18721 gcataacgat ctgttcttcc gggataaccc ataatcatgg caaaatctcc ttctttaacg
    18781 cctttaatac ttacagggaa atagtatttc gattttaaag gaacattttc tttagcatac
    18841 ttggcaggct ttccatcggg tgaagtgtaa atacggaaca tagaaaaatc gcctgtctga
    18901 cgtggccaca tccagttgtc ggtgtcgcca ccaaagtttc caatactcat tggaggtgtc
    18961 ccaaccaaac gcacatcttc atatctttcg taaacaaata aataatattc attaccacca
    19021 taaaaagcac gcacatcggc tgtataaaaa tcatttcctg cagttgcttc tttagtaagc
    19081 ttagaaaata ttccgggtaa tttagcttgt aaatctgctt cggaaagtga gtcgctgagt
    19141 tctgctttta ccttttgtgt tacatcttcc atgcggataa ggaattttac ggaaagaccg
    19201 gttgcagcaa gttctttatc gcgagcagtt gcccaaaatc catttgtaag ataatcatga
    19261 tctatactgc ttagtgattg aatgtaatca agtccgcaat gatgattggt tagaattaaa
    19321 ccatcaggag aaacaatttc gcctgtacat ccgccaccaa agtgaataat gccatctttc
    19381 attgaagatt tgttgatgtt ataaatatca tcagccgtga gcttaaagcc cattttttgc
    19441 atatctgcct cattaagttg ttttaatgtt gacagcagcc acataccttc atcggcatgc
    19501 aacatggttg taaaaaacag cgcacaggct gtaaaaaaca gtcgtaattt ataattcata
    19561 atttaaaaga ttttgagtgg cgaatatcag tatttttatt ttcatattag ttaaaaactt
    19621 gttaatgaca gaggaatata atccgttcat tagtttattc tgttatatgc cgataattta
    19681 acaatcaaat cttgacagaa gcaaataatt tttttcaaaa gccacgaatc gttttttcgc
    19741 aattctatcg tggtgaaaaa ggcagactta aaacttaata aagtaacatc tgagttattt
    19801 atttggattt gattgattaa atttgacaac aaatgtaaaa gtaaaattcc ggttatatga
    19861 ataagctaaa aatgtttttt attgcagctg caatcacgtt tgttttatca gggtgtggaa
    19921 caaaacaaag taataactca agtgtagaga aagtgaataa ggctgtaaat gatgagcctg
    19981 tacagtacga ccctgatgaa accatagaac tggataatgg acagaagtgg aaggtgaatg
    20041 aagagatgaa accttttcta ctaaaaggaa aagagatttt gaatgaatat attcagaacg
    20101 aagataacga ctatacagga ttagcagcag cactcagcga gcaagataat aagttgattg
    20161 aaagctgtac aatggagggt aaaagccatg aggaattgca taagtggttg catccacatt
    20221 tggacataat atcagatctt gagagtacaa aagatacagc agtagcaaaa aaacttgtgg
    20281 aacaattaaa agcttcctat gttgattttg aaaaatactt tcagtaagta ccgaaagtat
    20341 ctttcatttt tttatttacc cggtaaactg aaatcaaaag gtgattttac tgagtgtata
    20401 aacaagaagt tagtcgctgt tttgtacaac agatgttact gagttatacc acacaacttt
    20461 actcttaaat aataggacga taatataaat gacagaattt tttaatcaat tacttcacga
    20521 aattgaaagg ccacttacca atccggtact gatattttct ttaatactat ttattatttt
    20581 actttctccc attttactga gacgattaaa tattcccgga atcataggac tcattattgc
    20641 gggagtaatt atagggccac atggtttata tatcctcgaa aaaaattcag ctatcaatct
    20701 gttttcaaca ataggtttgc tgtacattat gtttattgcc ggtcttgaac ttgacctgaa
    20761 tgagtttaaa tcacatcgca ataaaagcct tgtgtttgga ttttttacct tcattattcc
    20821 tttggctatt ggatttcctg tctgtcatta tttgttacat tatgatttta atgcaagctt
    20881 tcttacagca agtatgtttg caacacatac gctggtagct tatccgattg tcagcaagtt
    20941 aggtgtttca aaaaatcagg ctgttgccat tactgttgga ggaacaattt taacagacac
    21001 tgctgtactg attatgctgg ccgttatcat gggcaacagt cagggaaatc ttaatcagga
    21061 attctggatt cgtttaggtg cttcattgat tattttttct gctatcatgt ttattgttat
    21121 accacgtatt gccaaatggt ttttccgaaa actggaaagc gaaaaacatt cgcattatat
    21181 ttttgtactt tcagtggtgt tttttgcagc ctttcttgcc gaggttgccg gagttgagcc
    21241 aatcatagga gccttcgttg caggattagc attaaacaaa ctgattccac actcatcagc
    21301 attgatgaac aggattgagt ttattggcaa ctctttgttt attccgtttt ttctgattag
    21361 tgttggtatg ttggttgatg taagcgtaat catgaatgga ccaactgcac tcatcattgc
    21421 gggcacattg acaattgtag cgataacagg caaatggctt gccgcttttc tgacacaagt
    21481 tatttttaaa tattcatcat cgcagcggca gctaattttt ggtctcagca gtgcacatgc
    21541 agctgcaaca cttgcagtta ttttagtcgg ctttaaagca ggaattcttg atgaaaatat
    21601 tcttaatggt accattattc ttattttgat tacatgtgtc attgcttctt ttgcaacaga
    21661 gcgagcggca aaaaaaatta tagtagaaac agaagatgac agcagtgaaa ttttaaataa
    21721 cagcagtgct tcaaacgaac atattttact tgctgtggct aatgttaaca aaatggaaaa
    21781 gttgttagac tttgccattt taataaagga caagaaatct gttaatccgg tgtctgttct
    21841 ttcagtagtt tctgacaata gcgaggcaga gaaaaatatt ataaaagcaa aaaataaatt
    21901 agaagatttt gtaaaacaag catcgggaac agaaacgaag gttaatgtaa ttactacaat
    21961 tgatcacaat gcttcaagtg gaattacacg ttgttcaaga gaaattatgg ctgatattat
    22021 tgttttggga tggccacagc gtacaggatt tcttgataaa cttattggcg agaaaattga
    22081 aagcatatta aacaacacgg ataagacaac attaatttgc tttcttgaaa aacctcttga
    22141 acttcataag cgaatagtgg ttgccatacc gcctcttgcc gaacatgaaa atggatttgg
    22201 tttatggctt actaaattag caaaactggc acaggaactt agcattccta ttcatggcta
    22261 ttgtaatgaa gtgactgaaa aatcaataag aagatatttt aataaagaaa aaatatcagc
    22321 ttcactccat ataagtttgt ttagtgactg ggatgatttt ctggtgctgg caagaaatat
    22381 ccatgcagat gattttgttg tactggtctc tgccagacgt ggtgctactt cttatatgag
    22441 tatattggat ggactcccat tgaagataga aaaacatttt agtgtcaata gtcgcttgat
    22501 tatttaccct caacaatatg acggaaatac taatctggaa aactacagcg aagtttcagc
    22561 agaaacttta aacagaggta ttgaagtggt acagaaaatt ggtaaagaaa taggctctat
    22621 gttgaaaaag aaaaatcagg agtagatttt tttctgatat ttcatttcga tttataaatc
    22681 ggaacggtag aacaaggttc gccatacata atgcttttaa caacaggttt tagtttttgt
    22741 gtcagcagca tataaacagc aggcgaaaag ttttcatcac cacaaccttt aataactata
    22801 cgtttgttgc tatagttttc aatattgatt ttttcaattg catcaatggc taacttttct
    22861 ttgagttgag tagtgttacc aaaaaaaatg gtggaagcat gctgttgcaa tgcctcgcta
    22921 atgagcatat atgcccaggt tggaataatg gcatcaacag aacaataaac agcaactgca
    22981 gcaccattat actgctgcca gttatgttgt ttgatatgct ctctgaaatc tttttcttta
    23041 agaatcattt cctcaaataa ccatccttta atgtcaagtt tgattattgt agtggcaggt
    23101 ataaaatctt ttggatctat ctgtataata ccactgtcgg caacgcggtt tttaatttcc
    23161 tgttccatga ttaccacatg ccaagttcca tgcgcgcctc ttcgctcatc atatctttat
    23221 cccagggtgg atcaaaagtg agttcaactt ttgcatcgtt tacatcagga atacttttta
    23281 ttttttgttc aacttccaaa ggcaatgatt cggcaaccgg acaagcaggt gaagtcagag
    23341 tcatctttac atgcacatca tttccttcaa taatttttac ttcgtaaatc aatcccagct
    23401 cataaatgtc aaccggaatt tccggatcat aacaggtctt tattgtttca ataaccctgt
    23461 catataattc ttttggctct tccataagtg caaatttaat ggatttattg ttgtcttgat
    23521 tttattgctg ctttattctg attttacatt ctatagagta ttcaaagtaa attcatgcag
    23581 tacagcaata ataatcccat atttgaaaac tttaaagtca gataaaagtt taagaaaatt
    23641 aaaataaata ttatgaagca gtttttggtc attattctta cagttggtct ttcattatca
    23701 tcatcagcgc agcaaaaaac agttttggaa gcaaatgaaa aagctgctat aatcagtttt
    23761 gaaaaaactg aacataattt cggaaagatt aaagaaggtg acaaagcaga acatgttttt
    23821 ctttttaaaa ataccggcaa cacaccttta attgttactg acgtgagtgt tacctgcgga
    23881 tgtactgctg cagaaaaacc tgatgcacca attatgccgg gtaaagacgg gaaaattaag
    23941 atagtgttta actctgcggg taaagttggt tcacaaaacc gaactataac ggttagcact
    24001 aatgcaaaga ataaaattac ctatttgaaa atgacgggtg aagtgagtga aaaataagta
    24061 ctgctaaatc agttaaactc tttctccaaa ctccatcaga aaagctttca taaactcatg
    24121 cagttcgcca tccattactg cttgtacgtt tgacgtttcg caatctgtac gcaggtcttt
    24181 caccatttta tatggatgaa aaacatagct tcttatttgt gagccccatt caattttttt
    24241 cttaccggct tctgttgctg ctttggcttc attgcgtttg cgaagttcaa gttcataaag
    24301 ttgcgaacgc aacatctgca tagctcgctc gcggttgcca ctttgcgatc gttccacctg
    24361 acacactaca acaatgcctg tgggtaagtg tttgagttga acctttgttt ctaccttatt
    24421 tacattttgt ccacctgcac ctcctgaacg agaagtctgc atttctatat cagcatctct
    24481 aatttctatc tctatagtat cgtcaatcaa aggataggca aacacacttg caaatgatgt
    24541 atgacgtttt gcattagaat caaaaggaga aatgcgtact aaacgatgta caccattttc
    24601 gctttttaaa tatccgtatg caaaatcacc ctctatttct aaggtaacag attttattcc
    24661 tgcaacatca ccatcttgcc tgtcaatttc atttatgcga tagttcattc tttcggccca
    24721 gcgtatatac attcgcataa gcatagatgc ccagtcgcaa ctttctgttc ctcctgcacc
    24781 ggcatttata gtgagcactg cgcttaatgc atcttcttca ccgctgagca tgtttttaaa
    24841 ttcaagctct tctaatgatt tttgaacaga cgtatattgc tcttctactt cttgttctga
    24901 agcatcgcct gtttttgaaa aatcgaataa tacgcctaaa tcatctacag cttgctgtac
    24961 ggcagcaaat gtgtcggtcc agtttttttt aagcttgatg gattttagaa tctgctctgc
    25021 cctagtcggg ttgttccaga aatccggtgc atgggtaagc tgttcttctt cttttatttt
    25081 caggagtttg acatcaatgt caaaggtgcc ccctcaaagc gtcaacgcgc tccttacatt
    25141 ctgttaattg ttcttgtgtc ataccggctg cgaatttacg aattaatgct taacagcttt
    25201 ttctgactat gtagttttca tcagcttata agctttggct acatcaaaaa tcataaacag
    25261 aaaataggcc atcaaaaaac taaccgcaaa gggtatagct gtatcagggt ttaaaaacat
    25321 atagctaaca ataactgtaa gcatcagaaa taatttaaac gctgttgatg ccataaaaaa
    25381 tcgaatggac tcattacctt tacttttggc aagtcgcaaa taatgtatga cagaaaaaag
    25441 tatgaagaaa aatatcccgc acttccacca cgaagggtag aaacgtgcag ccagaaaatc
    25501 tttcatcaga taataaagta aaatataaag cagggttatg ccggcaagtt gcaaaatgta
    25561 ggtgatgaaa cctttgttca ttttttctta tgtaaaaaat ctttcaatgt taagtaaaga
    25621 gcagcagcaa cagcaattaa agcaaaagta acagtgaata gaggaatttt gttttggcaa
    25681 tatttgtcta agcggtaacc tccataggta ccaataaaca taatagcacc catttgaaat
    25741 cccatggatg cataccggac ataatcgtta agctgatttt tcctggagtt tggcttctga
    25801 tctttcgaca ttttttatgt ctttaaccat aggccccata ttacagttgc ctgtaaaact
    25861 tgccccaact tcaactacta atttaccggt gttaatgtcg ccattgattt ttgcagttgc
    25921 ctttagaaat aacatttcag taacggtaga agttcctttt actgttcctg aaacatctgc
    25981 attctggcaa acgatgtcgc catccactac tcctgttgtg ccaacaacca ctttagcttt
    26041 tgaaaatact gagcctacaa tgtgtccgtc aatacgcaag tcaccattcg acttaatttc
    26101 accttctatt gaagttccgg cagcaattaa gttaattgag cctgatgtag tttcaccgga
    26161 tttaacggtg gtggttttgt tcttcaaatt ttcaaacata gtaataacct ttaattaagt
    26221 attgcagcaa agataaaaat aaaagttatt aacaaatgca aaaattatgg ataattaaca
    26281 cggtagaaat caaggtattt gctcaagtct ttatctgtaa tgaacttgtt gaaatttttt
    26341 tctgtaatag ggaaaagtgt aaaagcactt ttatccatac cctcaaaaac cgtttggtca
    26401 gagtctaacc cttgtatata ttctttgctt tgcgtgtggt cggggaatga acgcaccatc
    26461 acataatttt tatcatcaac tcttaactca gtcacattca ggcttttctc tttgtaattg
    26521 cgctgattaa acttatttat tttttctttt aaggttgtgc cataaggtaa ctcagcagaa
    26581 aaaataatca gcacattgtg cggtttgtca ggcataaact catagataga attcagcatt
    26641 ttattacctt cagcagtttg tgatgttgcc gattcgctga tggtagtatc tattggagct
    26701 acgttattca ttttatcgag aatagctttt gccaatatgc tgacagaatc tcttgggtat
    26761 gcagcaacta tttttttaag cgaagcttca aactgcgggc gaggttgtgt atatccgata
    26821 gaaagtgcct gtaggtaggc aaactttggt gctaatgtac ctccgggaaa caatgagtct
    26881 gctgcagatt tacgctctat aacatctgta tattgacgat tgagaaaagc cttgtatgta
    26941 ttttcgtaaa acacctgcat tatggctgtc ttgcgctggg catctttaaa atagttgggg
    27001 ttggttatga tgcgtgcata ttccgaatcg ccatagtcat tcagaataat ttttttataa
    27061 tactcggctt tgggttcatc aaataatgcc agataacaac gatacagatt gtaataagca
    27121 ggtagctcat atttattatt cggatatctt ttcatcatct cttccatatc gtcagcagca
    27181 ggctttggct gacctaaagc ctctttataa atgattcctg cattgtagta tgcttctaat
    27241 attttagcat tgctttcttc tttttgttgc gtagtttttg ggatgttgtt aaagtaagct
    27301 tttctgcgag cctcgctgtt caactgtctt atgctgtcgt ttaatgcttt ctgtgcctgc
    27361 tctaaactgt cggcacgagc ttgcgcttcg gcttcttctt cactgccaaa agttgcaatg
    27421 gattctttgc tgcttctgcg ccagttgtct tcgagttttc tgttacccca ttttttagca
    27481 aactccgaaa acccaaaact aatggcactc tgattataaa aataccatga gctaccggtt
    27541 gagttaccgg gcataccatc ttttcccggt tgattttgaa actggctgaa ctgcatgttg
    27601 gattgctctt cctgctcttt agctttggct gcttcctctt cctgtttttt acgtagtgac
    27661 tcagcttctt cagcagcaat gatatcatct atgcgagaat attgctctgt ttctgaaagc
    27721 gctcccaagg tgagcagact gtcttcatgt gaaattatat tcaggttttt tatcaatcgt
    27781 gaaagattgt ttttcttatt ctgaatcaat gcataatccg gatagtcaga agtaatgttg
    27841 gtagcagcac tgtcgtaata taactgtgcg gcacggaagt caagtttgcg gaaacttatt
    27901 tcgcccattt ccaagtatga aatacctttc tgattagcgt tgtttgtgct cgatgcaacg
    27961 gacttcttta aaaattgaaa tgcctctgta gtatctttct cgcgcataga aattccgctc
    28021 agcgcatagt aaatctgatc aagataatct ttgttttttt catccttcag catcttgttc
    28081 aactcatctt ttacttgttg cgcatctgca ctgtttacat caaaagaacg cgctctgttg
    28141 atttttgcat taaatgccat ttcatactca ggattcatct tgattacttt gcgataaatt
    28201 gcaaaagctt tctcgttcga atcaactttc tgatatatct gtgcaagtat ataattgtaa
    28261 cggattttgt cagctcgctt agtagccaat tctgcagcct tgtttaattg ctcaatagct
    28321 ttaggataat tttctttttt cagataaaaa tctgcagcaa cagcagcaaa gaggggtttg
    28381 tactttgaag ataattgctt ttcatttttt aaatagtcga gcatatattc agcatcggcc
    28441 attttgccca attgcatttg cgtttgcatc tgccaaatta aaccatcaaa tctttttgga
    28501 ttatctttat aggtagaggc aacatattga aaggtttcta atgcagtcca gaaatcatgt
    28561 ttaagaaatt gtgctttgcc tattaccatg tagttgtcag gtatccaacg gcaacgttcc
    28621 ttgccattga taaacatcga gtgtcgttga atgactatgg agcttttttt tatggcttca
    28681 tccatgtcag gataaatggt tttggctttg tcgtcagtag catatttaaa aacatcaagc
    28741 acccggtcgt aatgatctac atgcaatgaa gctaaagttt ttgtgccttc tttcactctt
    28801 tcgcgggcat taaaatagcc gttgtagtgt gccgtaagat tatgatatgc cctgttaacg
    28861 acagtgttct tttgtgtaga acatgctgat agaaacagcg taagggtgat aaaatatttt
    28921 aagtgattgg atgatttcaa aacaatatag ttattgcgat aaatattaac tttttccagc
    28981 gaaaattatt gctcgtttaa ttattacgat atttgcccga ttaagaaggc aaacataaca
    29041 atactttttt gtttaaacaa cgcatggcca aggcatcagg caaagaaaaa gtcaaactga
    29101 gcactaaact ccgcaacaag ttccggttag ttattatgaa tgacgaaact ttggaagaaa
    29161 aagcatcttt gctgctctct cccatcaatg tggtggtggt ggtgggcatt gccatcatta
    29221 ccctcattac agccacaacc tatattattg cttttacacc gctgcgtgaa tatattcccg
    29281 gatatacaga cgtaaaagta aagcgggcag ccattgaaag tcttatccgt accgattctt
    29341 tacaaaagca ggtgtcagcg caggatttat acattcagaa tctgcaaaat cttattaccg
    29401 gtcaatcaca gaaaaccgtt tcgcttgaca cggtttctgt accacaactt tatgatacca
    29461 taaagcaact acttcattcc gaagaggatt ctttgctgcg aagtcagata gagtcgccac
    29521 aggattacaa tttaacgttg aacgctggaa ccaccaaagg ctctattgcc aacttttact
    29581 tttttgctcc attaaaagga gccataacta cacggtttaa tccaacagaa aaacactttg
    29641 gggttgacgt agttgcaggc cctgatgctg ttataaaaag tgctatggac ggaacagtgg
    29701 taattgcaga atggacttcg caggcaggtt atgtcatagc catacaacac agcaacaaca
    29761 tgttttctgt ttataaacat aattctgcac tgcttaaaaa agtgggtaat gtggtgaaag
    29821 ccggagaagt tatagctatt gtgggtaact caggcgaatt gagtaacgga ccgcatttgc
    29881 attttgaact ttggtacaat ggtgcgcctg ttgacccaac agattacata gcttttgaat
    29941 aattatagca gatcaaagta attattttct gaaagtattt tttcgatcag ccttggatgt
    30001 ggaaaattgt ttttaaaatc ttcggcatca actgctttta actcaggaaa tttttttggt
    30061 gatataggtt tgcttaaatg tgcaacaatg tatgatgcat gaatatgttg atgtgtcagt
    30121 ttatgaatat gtgttcttcc tgattcatat tttgtaacac catagttttt tgcaaatttg
    30181 ctgatagagg ctgatgatat ttttgtttgg gtttcgagca atggaaattc atagaggttt
    30241 ttccagatgt cgtttccatt acgttgcctg atataaaatt tgtttctgta aagaagtatg
    30301 ataaaacaaa agtgtcgcac tttgatttta atctttttac tttttaccgg aaaattgagt
    30361 tgttgctttt gttgatatgc caaacaatga ctttgcaaag gacaaatttc acacttagga
    30421 tttcgtggtg tacaaaccaa agcaccaagt tccatcattg cctgattgtg taagcccgga
    30481 aattttttat tgcctatgtt ctctgctaat tgctgaaaaa cttttttccc cacggaactg
    30541 ttaggggtgg ttttttctgc aaacaatcgt gacaatacac gagtcacatt accatcaaca
    30601 gtaaagacag gctcattaaa tgccattgaa gcaattgctg ctgcagtgta atcgcctaca
    30661 ccttttagtt ttttaagctc aagataattt gaaggaaatt tattgtgaag tattttggta
    30721 atatgttgtg ctgtggccaa aagatttcgt cctcttgaat aatatcctaa tccctgccac
    30781 aacttgagta cttcggtttc tgatgctttg gcaagatgtt tcactgtagg aaatgattcc
    30841 aaaaaccgat agtaataagg caatccctgt tctactcttg tctgttgaag aataacttcg
    30901 ctcaaccata ttctataagc atcctttgtg ctgcgaaaag gtaactcgcg cttgttgcgt
    30961 tcataccata aaataagttt gtttgaaata ttcatttgat ttggcaaaga aagtcattat
    31021 aaaaaatagt tacagatata atttacccga caattcagct acagttaaat ttacttatca
    31081 ttgccttcgc tttttgttat gaaattaatt aaggacatac actcactatc tcaaattgtt
    31141 agcagcaaga aacacgagca tatcaacatt ggttttgtac caacaatggg ggcattgcac
    31201 gaagggcata tttcattaat taaacaatcg ctgcttgaaa acgattttac tattgccagc
    31261 atttttgtaa atccattgca gtttaataat cctgatgatt taaaaaaata tccacgtgcg
    31321 ttagacagcg atatgcagat gcttgaatcg gcaggctgta atgctttgtt tgcaccggaa
    31381 acatcagaag tttattcagg agatgaacaa cccttgcagt ttgatttggg caatcttgca
    31441 aacattatgg aaggtaaatt ccgtcctgga cattttgccg gagtaataac tattgttcat
    31501 aagctatttt catgggttaa acctgatgtt gcttattttg gagagaaaga ttttcagcaa
    31561 cttgccgtta tcagaaaaat ggctaaagca ttacatccta atgttattat aacaggttgc
    31621 cctacacttc gtgagcccaa tggtcttgcc atgagctcgc gcaatgtcag attaaacaaa
    31681 accgtacgtc ataatgcatc tataatttac aaggctatgc aacatgcccg acaactgaaa
    31741 aacgtactgc ccgtttacga agtttgttca aaggtgagaa gtatgattga agaagtagct
    31801 ccttttaaag ttgaatatat tgaaattgct gatgcagtaa atctacagcc tttacaacaa
    31861 tggtcagaca cacagtcggc acgaattttt gttgctgttt ttgcgaatga tgtaagactg
    31921 attgataacg cagcgctgta aaaaaattat accgtgcaat gccttgcaaa gacacctaat
    31981 ggaagtcttg ctcctgcagt gctttgcaga aaaatttctc ccgtctgtcg tttgatgttt
    32041 ttactaaaat gttcgtcaag aaggttatct aaaattattg atgaaaaacc aagtgaatag
    32101 gtgttgagga caagaaagtg attttcttca agcagcaact taacgttggc aagcatttca
    32161 tttatctcat cttctaattt ccagttttcg ccatttggcc catgaccgta ggcaggagga
    32221 tctaaaataa taccctgata ttttttcccg cgttttactt ctcgttttac aaatgtcatg
    32281 gcatcttcaa ccaaccagcg tatgttatcc aacccactca gtgtcatatt ttcttttgcc
    32341 caagtaataa cctgttttat tgagtcaaga tgaatgatgt cggcaccggc aactttagca
    32401 gcaagagtag ctgcaccggt ataggcaaag aggttgagta cttttggttg cactacatct
    32461 aattttttga cggaatgata aataaaatcc cagtttactg cctgttcagg aaaaacgcct
    32521 acatgtttaa atgatgttaa tgccagccta agtcgaattt gttttgcatc aggcaatgca
    32581 taggaaatat gccatcggtc gggaatctct ttaagttttt tccatgtacc tgacgagctg
    32641 tttttagcta caaactttac atgtgcttct ttaagccaat cagcctcttt tttttgtctg
    32701 ttccacacgg cctgtggctc gggtctgatg gtaatgtact gaccaaaacg ctctaacttt
    32761 tcgaagtttc cgctatcgag cagttcataa tctttaaaat tttgcggtgt aagtaattcc
    32821 atacagaagt tcatttaaat gcctaaaagt aatttaatag caaccttttt attcagccag
    32881 cgtaaaacaa ggcaatatta agtctaaaaa atgataatca acacatcaga gctgtcgggt
    32941 cgtagcatac aagccacaga caaagcacca ttaaaaattc tattagttga tgatattccg
    33001 gccaacttgt taaccctcga aggcattcta gaagatgaaa gcagaatact tataaaagct
    33061 ttttcaggaa atgaggcttt aaagttactg atggaagaac ctattgattt gatattgtta
    33121 gacattcaga tgcctgacat ggatggcgtt gaggtggcac aattgcttcg cgctaatccc
    33181 aagacaaagc atatacccat catttttgtt tctgccattg ccaagagcga acgcccctcg
    33241 ctcgaggagt ttgaaaccgg gtcgtttgat tttattccaa agccattaga tattgaagag
    33301 acacagaaaa tagtttcaat ttttgaattc ggttgccgct ataaactgaa gtacagagag
    33361 caggaggaaa aactgaaaag gctgaacgat catcttgata aatttcttta catcctctcg
    33421 catgacttaa atgcaccatt gcgtgccatt gagaatcttg tgcagtggct tgaagaagat
    33481 ttgcaatcac agatttctga tgagagtaaa gaaaacattg ggctgcttag aaacagagta
    33541 gcgaagatga aattacttct tgatggcttg ttacgttatg cacgttctgg caacacaaaa
    33601 catcatcagg atatgatttc acctgaaaaa atgatcagac aaattgtagc tgagctgcag
    33661 ggtgatagaa aaatatcact gaatattgat tcttcagttt tagagatgca aacagacagg
    33721 acagcattgc aacttgtgct gaaagagctg attcaaaatg ccatattttt taatccttcc
    33781 gataaaagca ctattgacat ttctatttca gagaaaaatg attcttatga atttgttgtt
    33841 gctgataacg gccccggaat aaaaccacaa catcaaaaaa ttattttcga actttttcaa
    33901 acacttccag catcggtagt accctcatca ggtgtaggtc ttagcattgt gaagaaggtg
    33961 gttgaagaat atggtggaac tgtgtggttt tctccaactg taaagtgcgg tacagaggtt
    34021 cactttaact ggaaaaaatc taagatttaa aatcctgaca gccgtgcttt tattgtactt
    34081 gcaccaacag taaagagtac aagactgata gtgctaatag gcattaatat agctgcaaac
    34141 aatggcgaaa gtgtaccgct gacagcaaag gctaatccaa caaaattata gagtaaagaa
    34201 ataataaagc tgatgataat tatcgttact gctgatttcg ataaattaat cagttccggt
    34261 agttgacgta atgcagtagc atccataata gtgtcgcttg caggagtaaa gttattggtg
    34321 ttgtcggtaa cggaaattcc ggtatttgct gcaagcagag cacctgcatc attcaaacca
    34381 tcaccaatca ttagcacatt tttcttactg tttattaaat caataatgta gtttctttta
    34441 tcttcaggtt tttgattaaa tcttaattcg gcattaccca ataagctggt caggttattt
    34501 ctttcacttt cgttatcacc tgtaagtacc actaaactat ggttgtgatt cagtttatga
    34561 gatatggtgt tcaacccatc acgataaata ttactgaaaa gaaatttacc ctttttaaag
    34621 ttgtcaatat tgataaaaac tgctgttgca ttttcctcat tttcattatt tacttctgcc
    34681 cagtcgactg ttccggcttt aatgttttta ttatttatta ctgcactaac gcctttaccg
    34741 gcaacctcta caaagttgtt gcatggataa aatttattgg cttgcaaatg ttgttgcagt
    34801 aatttgctta acggatgtga tgattgtctg aatagcgata atagcattgt tttgtccaca
    34861 tcatccagcg gatgtccatg ataacttatt tcagaaacat tgttcttggt taatgttcct
    34921 gtcttatcaa aaactatagt gtcagcatca gcaatttttt cgagtacacg atagttgcgc
    34981 atgtagattt tatttctacc caaaatgcgt aggatgtttc cataagtaaa aggtgcagat
    35041 aaagccaatg cacaggggca ggcaattaca agcactgctg taaatgcatt aatggcttta
    35101 tgaacatctg tattccacca atagatagca gcagctgtag aaatagaaaa agttactaca
    35161 ataaaccaac ggctgatggc attcactaac ttttcaaacc tgcttatatc ttgtttattc
    35221 cagttgctat tccacaattg tactaagtag ctttgcgaaa cttctttcat cacctgaagt
    35281 tcaatcatgg caccagcctg tttaccgcca gcataaatat attcaccggc tgcatgtgaa
    35341 acgggtagcg actcaccggt tacaaaatga taatctaccg aagcattgcc tttaagtaaa
    35401 atacaatctg ccggaataat ttcattgttt ctgataagga ttctgtcatt aactttcaaa
    35461 tcattaatct gtacttcttt ttcgtggcca ttttttatag aagtaactgc tatgggaaag
    35521 tacgatttaa aatctctgtc gaaagctaaa ctatcaaaag taaaatcctg aaaccagcga
    35581 ccaataagca taaagaaaac aagtccactg ccgctgtcaa aaaatcctga acctgtttgt
    35641 gtaattattt cataagcact ccgtaaaaaa attactgtta tacctatggc aataggtaaa
    35701 tctattgaag aggatttttg tttaagtgct tgccacgatt taataaagaa ctcgtttgca
    35761 ctgtaaaaaa acacaggcaa tgcaagtatt aaaatcagat aactgaatag agtttgtaat
    35821 gaagcatcaa tattatttcc ggaagaaaag tattcaggaa aactgagcat cataatattt
    35881 cctgagcaaa aaccggcaac accaattctg ataatacgac ttctgttggc tttttttgtt
    35941 tttttctttt caatagcatc aagttgcagt gccggttcat agcctgtttg tgagagtttt
    36001 tctgctattt ttcggagtga agttttatga tgattgtaaa ccaaacgcaa ctcttttcga
    36061 acaaaatcaa ctcttgaaga aagtactcct tcatccacct tctgaaaatt ttcgagcagc
    36121 cagatgcaac tgctgcaatg catagccgga atgtaaaagg tgatgatggt ttcattttta
    36181 tctctgaaac ttatcaattg attttcaata gcagggtcat ctaaaaaggc taatcgcttt
    36241 tgaagaatat cagactgttt aatgcccgga ttgttgttca ggttgtaata gttacacaaa
    36301 ttattttcac gcagaatttc ataaaccaac ttacagccgg tacaacaaaa agtatgctca
    36361 tcgcttgtga taacctgttc atcacagtca tcaccacaat gatagcattt cactttattt
    36421 acagtatctg tattgctcat agcctccaac ctaatttgcc tgacaatatt acaattgata
    36481 aagaatgtag gtgttgatat taattcttgg taagaatgac taaagtcatt tcagttgttg
    36541 tctcgaaata aaacatttgc cacattcaag tcactatgca aattaagcaa ttttataatc
    36601 agggtcattc acaattcagc tatattattt caagcggaaa ggaagcggtt attattgacc
    36661 cggtcagaga catagaacat tatttgtcat atttaaaaga taaagaactt actttaaaat
    36721 atatttttga aacgcacata catgccgatt ttgtttcagg tcatttagaa cttgccgata
    36781 aaacgggagc aacaatagtt tatggacctc aggtgcgagt ggcttatccc tcatacagtg
    36841 caagacataa tgaaggattt aatcttgaag gatgcagtat taaagttttg catacaccgg
    36901 gacataccat ggagtcggtt tgttatttat tgaataatag cattggacag ccaattgcaa
    36961 tttttacagg tgatacattg tttgttggtg atgttggtcg tcctgattta atgagcggta
    37021 ataaaaatgc agttgaattg tcagcgtatt tatatcactc tattaacgat attattaaat
    37081 cattgcctga tgagcttgta gtttatcccg gacatggtgc cggttcgttc tgtggaaaaa
    37141 atccgggtaa agaaaagttc actaccattg gcgaacaaaa gaagagaaac tatgctttaa
    37201 aagatatttc actcgaaaaa tttaccaatg aaataacttc aggtctgagt gtgccaccgg
    37261 aatatttttt taaggtcatg agtattaata ttatgggttg ccgacctatg aaagaggtgt
    37321 ttcgtaacaa tctgaatttt ctatcaccgg taaatttcaa agcagaaaca caacgtgcgt
    37381 tgatagtgga cacaagaaat gaattagagt tttcgaaagc ctttattaaa ggttctgtaa
    37441 acattggtct tgatggctcg tttgcaacat gggcaggtat tttactcgat cctgaccatc
    37501 cggttttatt agtctgtgac aatggtagag aaaaagaagc cataggtgct ttagcacaga
    37561 taggaataga aaatgtgaga ggtattttac aaggaggaat taatgcatgg aaagatatag
    37621 gagaaccggt ggacagtgtt gagaatattg atgcagaaga tttttatttt ttgtttgaaa
    37681 atgaagggta cattttggtt gatttaagaa gccatcagaa ctttaaggaa cagcatataa
    37741 aaagaagtct gagtattcca ttagatgaat tacctcaccg tctgaatgaa tttaactcag
    37801 acactaaata tttattagtg tgcaaaggtg ggtatcatag tatgatggca gcatcaattc
    37861 ttaaacgtaa tgggataaat caggtggtaa atatttcagg agggtttgat gcagtaagtg
    37921 catttcagcc acagtatgtg gagtcggtat agtctgaaca gaagtgtatt ggtaatataa
    37981 taaggggtag ataaaaacag attttgttca catgacttta aatttatgac aaaagtcgct
    38041 ttatgggttg cttaaagtca tttttagatt ataatgaagg aaatactttt acatcgttaa
    38101 ttaaaaggaa agcaataaaa ttccggtttg aaaatacgct tccgggttaa aataaaaata
    38161 tttgatttag tgttagggtt gcaaaaccgg tttgcttcgg caaccggttt ttgcttaata
    38221 ggtatatcga agaaaaataa gtatggaaaa gattgttgtt actactgact tctcggaatg
    38281 ttctgaaaat gctttgatat attctttgtc gctatgtaaa gagttgaagg catctttgta
    38341 tttgcttcac gtttttcata taccggttcc tgtaaccgac atgccctata tttcagtttc
    38401 gtctgaggaa gaagaaaaga atattaaatt aagattggaa gaattaatta cccgtgtaag
    38461 gcataagaca ggttatgagg gtatgattca cactataacc gattttaatt ttactgttga
    38521 tggtattctg aatgttgttc atgatacaaa tgcagatttg cttgtaatgg gacaacgtgg
    38581 taaaacaacg ttagccgaca ggctgtttgg aagtactgca acagcaatta ttaatcttgt
    38641 atcttgcccg ttattgctta ttcccgaagg atgtagtttc agaaaactta aaaaaatagc
    38701 aattgcctgc gatagaaaaa caggggacaa taaaaataaa gtgtttcatg tcgcagaaat
    38761 gattgctaca ttaaaatcag aaatacttct gtttaatgtt gtgcaggatg aaacggagaa
    38821 agataaactt agaagagatg atttttctga aatggccggt aatgatgtaa aattattttt
    38881 ccctgtacat catgacatac atgagggtat tttgcagttt gtaagtgata atgatgctga
    38941 ttggttgtgt atgtttccac atcgccataa cattttcgag cagtggttta ccagacttaa
    39001 ctcgggcaac atttcgcagc aagtaaaaat tccattattg ttaattaacg agttggtaca
    39061 ctaaacatat tgctgcaaaa tacttttagt aaaacaatat ttttaaatat aacagagaag
    39121 ttgttacgat tcaatttaag atatgaagca taggttgact attcaggcag aggatactat
    39181 tttagatttt caaaaaaaat tcaacaggat ttatccgttt cttaaaattg aattttttga
    39241 aaaagcacat atagcaaaac agggtagtca taaaagggaa atgattacat caaataggtt
    39301 gtttcatgga ttgataggac gaggaaaaac acatatcata aactttaatg acaattttag
    39361 tattagcgat tttgaaatgt tactgaagaa tcagtgtcgg ttatatgcac aggtttttcg
    39421 taaatcaggc aagatttggc ttgaaacaac agcaacagat tactggacat tggagcaaca
    39481 aaatgaagag ggcaaaagtt tagccaagca ctttaaaata gagtctgaaa atattaatga
    39541 tattgaacct tactagcaaa ggtatttact gtatctgtgg tgtttttcat gggtcattgc
    39601 taaataacag aaactacagc aatctcatat tatatgatga cttagaaaaa atttaatagc
    39661 aagcacatac acccagacat attttcacat tgtggtagca aactacctta ccgattaaga
    39721 tgccgctcct acggagctat taagatatcg ctcctacgga gctattaaga tatcgctcct
    39781 acggagctat
//
LOCUS       DAOQNP010000018        39423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_316_length_39423_cov_10.280761, whole genome shotgun sequence.
ACCESSION   DAOQNP010000018 DAOQNP010000000
VERSION     DAOQNP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 39423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39423
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_39423_cov_10.280761"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PKY09_04215"
     CDS             complement(<1..1307)
                     /locus_tag="PKY09_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12092.1"
                     /translation="MQKQIKIILIIVLLSSLKAKAQNTFEKIIDTLGSAGAYSIQETF
                     DGGYVFCGGSIYNGNDVCIVKLDSAGTIEWAKIYSGPSMEGANYIEQTPDSGYMVNAV
                     YDIGVPFARSWLLRLDANGDTLWTKVLSGGTGATIPSMVNSMASVNNAIYGLTGYFKQ
                     DTMPIVPRVFFIAVLSNGLVAANKTYNYSSTLSSDARAIDKTYDNGFIMAGAGATSSQ
                     YSDIYVIRTNGYGDTLWTRTYDKSTSDAAMDVKQTTDSNFIIAAITYDTTVYKNNIYL
                     IKTDAAGDTLWTKKYFYPLHQVVYSIDQTTDGGYIMTGNTDNSNGRSLYLIKTNATGD
                     TLWTRTYNPNIDNYGYFVRQTKDGGFIICGATSTGSYIIKTDSLGMVGNGTGIAEVNN
                     PFDFSVYPNPSSGNFTILFKGIPRKNAELKIYNIMNQGVYTGT"
     gene            complement(1608..4694)
                     /locus_tag="PKY09_04220"
     CDS             complement(1608..4694)
                     /locus_tag="PKY09_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNB12093.1"
                     /translation="MKQYTKFNNISGWAVFAIAAWVFLSTIEMTGSFWDCGEFIAAAY
                     KLQIGHPPGAPLFLMLSRIMTLFAGSDLQSIPIMVNAFSALMSAFAVLFTFWTITAIA
                     RKIVFVKNQEPSLAQILTVVASGVVGAMAFTFTDSFWFSAVEGEVYATSQFFTCIVVW
                     AVFKWENVADEKHSIRWIILIAYLMGLSIGVHLLNLLCIPALALVYYFRKYPFSWMGV
                     AKTMGISLVILMVVQYGIIAGFVNIGAKIDLWFNQSGSSLWTGFVVYSLIILAALTYG
                     IYYSQKKQKALLNTVLLSFAFLLLGYTSYAQIVVRSLANPPMDENNPENAFALLGYLN
                     REQYGDRPLGYGQYYDAKVVGQTEGAMSYTAIDGKYIPTGAKVIPEYDPARCTVFPRM
                     YSREPNHVSAYKEWSGIKGEQKPEFSNNLKYFFSYQMNHMFWRYFMWNFVGRQNDIQG
                     HGSILKGNWISGIPFIDSMFLGPQDKLPDSMKNNKARNKFYFLPLLLGLAGLVYHYKK
                     DMNNANVVMLLFIMTGIAIVIYLNQTPYQPRERDYAYAGAFYAFCIWIGLGVAAIADA
                     LKKRMPETTAAMLATLLCLVVPTVMAKEGWDDHNRSYRYTSRDFAYNYLNSCAPNAVI
                     FTNGDNDTFPLWYIQDVEGVRTDVRVINLSLLNTDWYIDQIKRKAYESDPVPFTLKTR
                     QYVQGTRDYIPFYDRSVQGYSDLKDIIEFVTSDNPEAKVRSEGGDEFNYLPTKKFRIK
                     VDKEAVIKNHVVEAKDTSKIVDYIEFEISKNYIMKADLMILDLIAHNDWTRPIYFAVT
                     VGNDSYLNLENYFQTEGLAYRFVPIKAAADPSGQTGSVAAERMYDNMMNKFMWGNMKD
                     ERVYLDQNNLNMTMNFRNNFARLSETLLAENETDSAVKVLDKCLVEMPDKTVPYNIMM
                     LRIAELYYRSTMNNVLPDSLQMLSNDVELKKTDSKESIEKGNTIVKRLADIYQNDLQY
                     YLSLKGTPYAKSVERETNQALAVFQELIRLSKLKDQKAITDDLQKRFEQLEAQYSR"
     gene            4898..5620
                     /locus_tag="PKY09_04225"
     CDS             4898..5620
                     /locus_tag="PKY09_04225"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNB12094.1"
                     /translation="MNLVIDIGNTRIKAAVFDGKKLIACHIIKSFKESSFTALINRYA
                     IRAVIFSSVVKEEKILGASEILQVFYLNHNTALPIVNDYATPATLGTDRLANACGAAL
                     LFKNKNCLTLDAGTCLKFDLITADAHYKGGAISPGLNMRYKALHDYTGRLPLVKQTDN
                     VALTGNTTQTSIASGVQNGIIAEAEYLIAEYKKLYKGLKVVVTGGDMAFFADRLKTHI
                     FAAPDLTLVGLNSILNYNLSRQ"
     gene            5633..7018
                     /locus_tag="PKY09_04230"
     CDS             5633..7018
                     /locus_tag="PKY09_04230"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB12095.1"
                     /translation="MQKQKYLFTVLFLALGLTLHAQTGTETPYSRYGIGELLNSGFAR
                     QSAMGGLGAAFQSSSAVNFSNPASYSSIRISTFETALRGELAQTSTSDLKQTKKGILL
                     NYLSLGFPVMKDNWGASFGLIPVSSTGYNLKESGQVINPNDTITTDFTKYYEGNGGLS
                     RFYIGNGFAPYSKTLDKYYASQHYKDLKEKGDTVTISKEVRWRKIVKGFSAGFNASYL
                     FGSLNNVRRVKFSNSAFINTKVTESTAYADFYFDYGIQYRYDFSEDNKLIIGISGAGG
                     TNIKGRRDVLWTLVSSSDFEANIDTVSLSENEKGKAFIPSFINAGFMFQQGEKWLMGA
                     EYATQQWSRYKAFNSNQKLKDSWRISAGAQYMPTNKLAYRAGIKVEQSYINLNNSRVN
                     DYSISLGVGVPVRLTSTERRLFDNRTMLHFSIEGGQLGTTSNNLVRQQYVRLYVGITA
                     NELWFIKRKYD"
     gene            7121..8836
                     /gene="recJ"
                     /locus_tag="PKY09_04235"
     CDS             7121..8836
                     /gene="recJ"
                     /locus_tag="PKY09_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNB12096.1"
                     /translation="MNKRWTLLKQADNDKSIKFGKELNINPVLANLLIQRSVNTFDEA
                     KEFFRPSLTLLHDPFLMTDMDKAVARIDKAIAKGEKVLIYGDYDVDGTTAVALVYRFF
                     KNRFAECNFYIPDRYTEGYGISLQGIDWAKENGFSLIIALDCGIKAMDKTKYASELGI
                     DFIICDHHRPGAEIPQAAAVLDPKRVDCAYPCKDLSGCGVGFKLLQAFAKKNNIDEQQ
                     VLDLIDLVAISIAADIVPILGENRILAYYGLELINKNPRTGIKAILELNAIRRELNIT
                     DVVFIIAPRINAAGRMDHGRSAVQLLISDSVGDAITESEKINKNNIDRRDADKLITEH
                     ALQQISRDNTFESKRSTVVYSAEWNKGVIGIVASRLTEKFYRPTVVLTDSNGHIAGSA
                     RSVKDFDVYNAIEACSEYLDQFGGHMYAAGLTLKHENFEAFKQKFEEVVCATIEEHML
                     TPEIEIDAELKLESITKPFYNVLKQFAPFGPGNMSPVFVSRNCIADKNQTRPVGQENK
                     HLKLAFTQDGFGKNFSAIGFQLGEYADLLKSGKRFDVCYHIEENHFNGNTELQLNIKD
                     IKISE"
     gene            complement(8828..9136)
                     /locus_tag="PKY09_04240"
     CDS             complement(8828..9136)
                     /locus_tag="PKY09_04240"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNB12097.1"
                     /translation="MAKKFSVKTNLHRRDIRGVSTYVVLREASDHFKETGIIEVVGFI
                     DNHPIEGALQPKTDGSHWLTIKKEWREAIGKTIGDSVEISIEIKKKIPPPQKVKKKLF
                     "
     gene            complement(9242..11506)
                     /locus_tag="PKY09_04245"
     CDS             complement(9242..11506)
                     /locus_tag="PKY09_04245"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNB12098.1"
                     /translation="MAFDIEMIKKVYVEMPARIEQARKKLNRPLTLTEKILYSHLSPQ
                     FEVKNYERGKAYVDFNPDRVAMQDATAQMALLQFMQAGRPQVAVPSTVHCDHLIQAKD
                     GAAKDLASALDKSKEVFDFLASVSNKYGIGFWKPGAGIIHQVILENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMAGLPWELKMPKLIGVKLTGKLSGWTSAKDVILK
                     VAGILTVKGGTDKIVEYFGEGAESMSCTGKGTICNMGAEIGATTSIFNYDRKMADYLR
                     GTGRADVADLADGIAAHLRGDSEVYQQPEKYFDEVIEINLSELEPHINGPFTPDLAWP
                     LSKFAAAVKENGWPAKLEVGLIGSCTNSSYEDITRAASLAKQAAEKGIKAKSEFTITP
                     GSEQVRYTVERDGYLGIFEKIGGVVLANACGPCIGQWARHTNDPNKKNSIITSFNRNF
                     AKRNDGNPNTHAFVASPEIVTALAIAGDLTFNPVTDFITNDNGEKVKLAEPQGIELPI
                     KGFAVEDAGFQAPAKDGSKVDVIVSPTSDRLQLLAPFAAWEKSDLKGLKLLIKAKGKC
                     TTDHISMAGPWLKYRGHLDNISNNMLIGAVNAFNDNTNSVKNQLDGSYDEVPKVQRAY
                     KAKGIGSIVVGDENYGEGSSREHAAMEPRFLGVRAVLVKSFARIHETNLKKQGMLALT
                     FADKADYEKIKEDDTFDIVGLTTFTPGVPLTLVLHHSDGSKEEIKVNHSYNEHQIAWF
                     KAGGALNIIRSEQA"
     gene            11699..12778
                     /locus_tag="PKY09_04250"
     CDS             11699..12778
                     /locus_tag="PKY09_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB12099.1"
                     /translation="MKTLQKQIITICILLSLTGIVFVSCKKDKDNNTAAPATEYGVIT
                     GKVHSPKNVAIASAKIISGEYSTISNKQGEFSLSLPVGFHNLIIQTGSGHVFKTVTTV
                     QVNTNETIHLDDTQTELQQIKQIAFIPGVYDRIETIIIDTLGYTATAITVPSLDNLNF
                     MQSFGGIFLNCGVSGNLDSLKYANLNNYVIGGGNIYASDWSVEFLTGDGFFRPSASSF
                     FTNSASKNQSHEAAYSTMSTCTSPLIGGFLPDSALCTAKYGPVTTVQNAHILDPDIIN
                     VLGKDSMDIYYNLNAWEVINLVDAPFTTIIEDTHFGLGSLAVACDLNGAMPAGHITYT
                     TFHNHPQGNISQDVKLVLQHFILNM"
     gene            12894..14117
                     /gene="lhgO"
                     /locus_tag="PKY09_04255"
     CDS             12894..14117
                     /gene="lhgO"
                     /locus_tag="PKY09_04255"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HNB12100.1"
                     /translation="MSNTSEYDVVVIGGGCVGMASAYKLNLRFPHLKIAVLEKEDRLA
                     AHQTGRNSGVIHSGLYYKPGSYKAKNCVEGRRELVAFCKEHGVAHDVCGKIIVATDEK
                     ELPHMNRVFNNGLQNGVEGIEKINAQQIKDIEPFCAGIEGIWVPCTGIVDYVGLTNKL
                     AELIEQQFTGSKVFTGHEVTAFVKQDGKTTVVTNKQKFTTKHLISCTGLFSDRMAKAD
                     GVNPNMQIVGFRGDYYDLTEKGMNKVRNLIYPVPNPEFPFLGVHFTRMIQHGVECGPN
                     AVFTFKREGYGKTDFDLKDTLQALAYIGTWKLFFKHWRFGLDEYKRAFSKKLFLERLQ
                     RLIPSLEMDDITPGRAGVRAMALDANGNMIDDFKIEYSGNAIHVLNAPSPAATASLAI
                     GTAVMEMAEKHFQIH"
     gene            14216..14716
                     /locus_tag="PKY09_04260"
     CDS             14216..14716
                     /locus_tag="PKY09_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12101.1"
                     /translation="MTTIKKPQDQPNKFYIISVSVLTFVFPAIGFWAEHFAIDTTLSF
                     DLFSKWFIFSAVGLRLFLAGIKQVINPEFTAKQIFQIDSPDSFPILRELGFANICFGL
                     TAIVSLFKPEWRIVSAFASGLYYGFASMQHGLKKALGMNEKFALWTDLLIFVFLLLYF
                     IKALSA"
     gene            14924..15295
                     /locus_tag="PKY09_04265"
     CDS             14924..15295
                     /locus_tag="PKY09_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNB12102.1"
                     /translation="MQSTIYKFTFLLISIILFNTGSHAQVSTTDTMMVYGNCNMCKKT
                     IEGSLKEKDGIIAKNWNKDTKMLTVTYDASKINKEQIGQKIADAGYDNQYATATEKAY
                     KKLHSCCQYDRPKQPSGGENK"
     gene            15317..16093
                     /locus_tag="PKY09_04270"
     CDS             15317..16093
                     /locus_tag="PKY09_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12103.1"
                     /translation="MKKQLLTLILLAITSLSMAQTATDFTANDCNGNSHNLFTELNSG
                     KVIVLCWVMPCASCTGPTLTTYNVVQSFQSSNPNKVFMYTCDDFANTNCTSLNSWANG
                     IGVMQQSWSLRFSNAAINMTHYGSPGMPKIVVLGPNHAVFYNSNNTVNATNLQNAINN
                     ALTTTGLNENNLPASSLTVLPNPAQNETKIKLNLLNNADVILECYNLEGKKVQEIFSG
                     NLNKGEHTISVNLEPFTNGMYLIKFSDGKQSQFINLIKSN"
     gene            16140..17282
                     /locus_tag="PKY09_04275"
     CDS             16140..17282
                     /locus_tag="PKY09_04275"
                     /inference="COORDINATES: protein motif:HMM:NF039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNB12104.1"
                     /translation="MHNKVQLKFSLVLLLICFSVSDAFSQCCAGGSGSPIAGGTSQGV
                     LQEKQVEISSNFQFISTNKFYRKNIVAPDSVRTFDGFKSTYEYFRVAYGITKNLTFSV
                     ESGYYYQKKEIGLNKDATTTYNAKGFGDLILFPRYDIINWTEEKHRTEVTVGLGFKIP
                     LGSYNDSTGNVEPFSGNTYYVTNPTSVQLSSGAHDIIFYTFLYRGFTEKNFRVFANAL
                     YIKKGYNPNGEKLGDFASVGIFAGKTFFNNLGVTLQARYEWMDRMKVNESIYLFGKPS
                     NYFPEATGYKKVFISPQLSFSKGKFTVYGSYDIPVYQYLNTSEHYTQMGSQHQATIGV
                     TFRFMALNSFLKEKSGVTYVCPMHPNVISDKPGKCPDCGMDLVKKK"
     gene            complement(17565..20513)
                     /locus_tag="PKY09_04280"
     CDS             complement(17565..20513)
                     /locus_tag="PKY09_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNB12105.1"
                     /translation="MKKLVAVLVFVMLCKASLAQTLTSVSPDSTYRGATLSALISGVN
                     TLFQSSSTTGFYLEQGTNSIIGSLSSMNALSETQTEIDFVIPYNAPYGWYDLRYVYED
                     TASQGTYLTLTLPNAVEILSANVTISGVVFEDLNGNGIQDAGEQGVSGQQILLTPDNT
                     LFTTNSNGAYMVNTYPGIKTITWQQSSGFVITAGSQASYTANFTAPVTGYNFGLSTTG
                     PVIISISADSMLQGTTLNAVISCAGVVLQNGSPLGAIISTYLTTPGGTPVVGGSNITV
                     INPTQFSVTFNSSGNLLAPGVYSLVLYYSDSLGVIHTLTLPNAVTVYAASMYLSGTAF
                     VDVNPDGLLGFQEPGMGGQTITLQPDNQTVQTDNLGNYTIPTSPGNKTITITPSTNTV
                     VNPPGAATISGNYSVTTTGLNFPLNTTDQYLTSMSPFSALAGTGFSAYISGANTVFQS
                     SSPPTGAIYDAYLFRTDTAVTYYANANTFNIIDDLHFYADFSIPANAPIGWYNLGVVY
                     DLTGFGNYITLNLQNALYIDQGSLFLQGNVFYDANSNGIKDVGEPGFSGQKVIVQPDN
                     VFAFTDNSGNYSVGSYAGTHTVQWQNTIPGFSLSSGSQSSYNVNASATTTGLDFGLNS
                     TLPVYDCKVVSRVGLRRCLMNNATIVNYKNTGYVTYDARITMKFSSNQTFIAGSITPT
                     SVNGQTVVYDFPGLTAQTTGSIIFQLGMPAAGSVVSDTIIMESLINGTVMETDTLISS
                     KIVSCSFDPNDKSVTPAGILSNHYTLMGSQLDYLIRFQNTGNDTAYTVVVRDVIDTGL
                     DLNTFALIDASHNVQTQVNLSNREVVFTFANIMLVDSNTNELLSHGFVRYSIMANQWL
                     PNNTTVFNDAAIYFDQNDPIITNETFNTMVIGLPTSVAEIPASSQSMVRVIPNPFNSK
                     ADIVFDNENNQTYHLVVYNTEGKEILRSSTKMNRIGIERKNLAEGLYYFRLLPEGNAK
                     SYSGKFIISN"
     gene            complement(20526..22571)
                     /locus_tag="PKY09_04285"
     CDS             complement(20526..22571)
                     /locus_tag="PKY09_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB12106.1"
                     /translation="MSIQKAASFLFALFMIVLTLSFSSSLPDPDHTLRLLTAAVFVFP
                     FSALILYGIYTKKISFSLFNASFAPVFILLGIVVITGIAWNKSLSSNDGLFEFIKWIL
                     LLFYLFVFSIINKLVADPMKMISRTVVVLLFIIQIYGVLQLISIWNYAHEHKEVFEIS
                     YRLTSLLSNKNSFAEMIALCLPLQVMCMLTDSNRWKKAAIVSLALSLVYLIILKSFSV
                     FVALVLVMIVLTFLKYSKIKSVITEKLSIKPERINLVTIAIIVSSMSGVILVLNKIDG
                     FRKAEIAVSYIKGNTTDVEFNANSVFERMMLWRNSIKLIAENFWTGCGLANWKLMAPK
                     FGFTGADYLTTDNIKFTRAHNDYLQIFSETGIAGFVTYILLLGWGITMAIKQYLTHQS
                     KEFLIILSGMIIFTTVSLFGFPMEKLLLMISVIAYFSLMVFYQMKDDRKKVMSKLVPA
                     VIVCFMVFALYSTKVTAARWYAESQYMQLLAAKEKNKWPIAYRISKSMDETYFPIDYN
                     GTPIAWYKGAAAFMNNQPKEALSYYEEAIAMAPYHVQILNDIGAVYMNNGDNDNANKY
                     FNKALAINPRFPDAKFNQAIAVFNKGNKLGAYNLFREVRKLHPPAKYADYMMVVIQHV
                     GDSIFRSVGFNPNVNPVWKIKYTGTNLWYYESIAMKQNKSFVQVIRHEIDSIKNINY"
     gene            22926..23861
                     /locus_tag="PKY09_04290"
     CDS             22926..23861
                     /locus_tag="PKY09_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12107.1"
                     /translation="MNTESLILSIRKNILWILIIALVAFVVSYKISGRLNNYYKADIM
                     FLLNDLNLTDDSPKENSISSVIKSKSLETERILAIIYSDALIQKVDVKLKLGKHYHLD
                     DKDPKYMTKLTASFVKNVTVEITSSKIMLLKVRDAEGPEFVANLAESIMNELLAFNRD
                     QTIELLNEKLAFNRALFNTLDSRYISKLKNLEQSVEQLRHNRPKEMTFNDFLIIKYNI
                     METTKQMGMLTSEIEDNIRSNLLINKTLEGKKLERIIVLNKKYLMPQSQNLMLNLSVA
                     TIVSILTILLLIVAIHSYGSNKDLIRLFIFGKKKE"
     gene            23931..24500
                     /locus_tag="PKY09_04295"
     CDS             23931..24500
                     /locus_tag="PKY09_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB12108.1"
                     /translation="MPTRQPEVYLTFDDGPEPEATPFVLSQLKKWNALATFFCIGNNI
                     EKHQELFNQIVSAGHQIGNHTQNHLNGWNNNLNTYMADVEQCSTHISSGLFRPPYGKV
                     TLKQIRSLRSQWQIIMWDVLSYDFETNVSPAKCIQNVMQHVRPGSIIVFHDSKKALKN
                     LSETLPVVLEKLSAKGYSFNAMPPYRPLQ"
     gene            complement(24472..25143)
                     /gene="rsmI"
                     /locus_tag="PKY09_04300"
     CDS             complement(24472..25143)
                     /gene="rsmI"
                     /locus_tag="PKY09_04300"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNB12109.1"
                     /translation="MLYIVPTPVGNLEDITLRALNVLKSCDIILAEDTRNASVLLNHF
                     GISKKVFTHHQHNEHAAVNELIRLLKEEQKTIALISDAGTPGISDPGFLLTRACIEHE
                     IELTCLPGATAFVPALVMSGIPCNSFVFEGFLPVKKGRQTRLKLLQEEIRTMVFYESP
                     HKLIKTLQDFITNFGADRKVCVVREISKMFEEKKRGAVAEVAEYYTNHPPKGEIVIVV
                     AGKEA"
     gene            complement(25173..25871)
                     /locus_tag="PKY09_04305"
     CDS             complement(25173..25871)
                     /locus_tag="PKY09_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12110.1"
                     /translation="MKRVQIIVLFISLLFCGIASAQIDSLGVIAISPQYPQCPDSAFM
                     QQPYINIQVTIKNYNANSTFSGGLMLKLQSDSLPEDSIILNSGTVYTIQPNSSLNILI
                     QSPYFFNSTNYKLGSNVVVVWPVSTQGGTVVKYNPYLACVLFLPFVGMNEYDRGYLDV
                     IPNPSNKYIDLNLPTDIGLKKVRILKPTGEEVRNYSVDLLKRITVEQLSAGIYFLEVV
                     TSDDHVYNTKFIKN"
     gene            25971..27440
                     /locus_tag="PKY09_04310"
     CDS             25971..27440
                     /locus_tag="PKY09_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12111.1"
                     /translation="MDILNQLINGLNKEEVRFFKMYINRVEKTDNRKDVQLFDYIRKS
                     GDRFSDEIIFKKLYQPQEKNSYYRLKNRLIREVNKSLTLQHFDDDMVVYIFRLLALVK
                     FYLNKNALKPAYFFLRKAEQKANETESLELLDIIYGEYIRLAREMTSINPEFYIHRRK
                     ENSEKLNQLRVIDDILAVVTYRLKITQNLSSKDETVISLLQKTTDDFIGDPKIVKSPL
                     FRFRIYNAISQLLLQSHQYTLLEDYLLKTLKIFNQEKLFTKNNHETKLQLLVYLVNSL
                     YKNNKLKKSLEYTNMLHQEMKAYNQLFYDKFLFYYYNALVINYSKTDKQKEIVTLQEM
                     EQNEKINANLFYRQFILLNFALAYFDQKEFNLSAKSFTRLQMMPDYKETDESLRMKVA
                     VAELMTRHELNDYSTFDYKLKQFKKDFRGVLNKPENEPEKLMSEILKLMMSVNNRKNR
                     AIELRIAQLKTFSKEEDQSGLINYLDWLQEKKLFKNKIR"
     gene            complement(27433..29823)
                     /locus_tag="PKY09_04315"
     CDS             complement(27433..29823)
                     /locus_tag="PKY09_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB12112.1"
                     /translation="MQKRLYIVFLMLLALAVAFFLAWFNYQNTTALSKTITLLQKPDT
                     RLSDLKELILNVNLAESHVRSFAITRNEKNLEDYYKQVEQTDSQLVKLKNLFPSNSQA
                     FDSLTASIHYKFDIYDKLIELRYRELINTAMSKISDKVEDVEIYQNIDSTETSKPGFF
                     KSLFSSKKKLRELERKALLLDSLNDLQAQKLSTIKQTVSEAKENEIRKVTQLSDGELL
                     LLEQDRLLKERIQSQIVLLEGDIRHNNENKSLEVISNSQQQLKTVVIATITASVLILI
                     LSIIILADISRANRYKIQLEEARARSEQLARFKEEFLATMSHEIRTPLSALTGFINRL
                     QKTNTTTEQQQYVNTINMAGQHLLNIVNDVLDLSRIETGKLNFKQDPFVIYKEVNDVV
                     TLLSQKADEKNIALVLEASNIRNLTVSGDALRLRQVLINVIGNAIKFTEKGTVLVKVE
                     KQEGELFKFEVTDTGIGIAEEKIAKLFDAYQQADNIERNYGGTGLGLAITKRLVELQK
                     GTISMKSVPEKGTTVTVMLRFPLTPMNETKITEKEFVSLHGFRLLVAEDDLLNRQLIT
                     AMLKDGGAEVITAESGKQAIEKILLYNPDGVLMDIQMPELDGYNAASFIREHISAMLP
                     LIGITANMMNDAKNECIAAGMNAVILKPFKLHELTEVLIPLIEKNREVQHKPLLSYSL
                     DSLFRTSNGKSDFVIRMLKVFISSNQSLIEKSIHFAKESDFQQSAAAVHRMIPGFRQL
                     DLDTFASILKNIEWLMLDGTEKNAATELLQQCAEQFEKLVAAIQSDIDRLEKEATT"
     gene            30049..30915
                     /locus_tag="PKY09_04320"
     CDS             30049..30915
                     /locus_tag="PKY09_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12113.1"
                     /translation="MKKLILIALPIVGMTLFGCENYKEQAAQLTRERDSLVSLNVAGT
                     QTIDEFITTLTDVENSLGKITEKQNSIAMTADRNPEAARNSRERLNAQIEEINRMMQE
                     NKDKVEQLTKKLKGANGSAAKFQKMVKSLQEQLTQKEQELALLNEKLAGLNVELATLK
                     TNFDSLSTTNQNQATVIADQTTKIHTAYYTVGNYKSLRDKQVLSKKGGVLGLGKKATV
                     VPAIKNDNFTKIDYTQISTIPVNSKKVELVTTHPDGSYKIEKNNDLVSDILITDPEKF
                     WSASKYLVVLTK"
     gene            31074..32108
                     /locus_tag="PKY09_04325"
     CDS             31074..32108
                     /locus_tag="PKY09_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNB12114.1"
                     /translation="MLKKIFFLILFSFGFIELFAQTEPAKATEKESLVNWISLNEAEK
                     RNKEIPKPIIIDFYTSWCGWCKVMMSSTYSDPSIASYINTNFYPVKFDAETKDTIAYD
                     GVTYKPTGTQKRDPNEFAVKMLNGTMVYPSTVFMNKSANFTMSAQGYLETKKIEPMLV
                     FTLENGFRNSSYEDFNTQFQKAFYDSLQTKVYESVKWQTPSHFFIKDQKPDEKKKIVF
                     INTEWCNTCRVMYRTTFSDTAVSSLLSKHFELINFNPETNDKLYFQDKEFENIHSKEM
                     PFHQLVYALSRNGLIFPQVIFMDEKNTVVDAIPFYLNPKVFKSIVRFYGEDIYKTKNW
                     ETFIKEQETK"
     gene            32166..33386
                     /locus_tag="PKY09_04330"
     CDS             32166..33386
                     /locus_tag="PKY09_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNB12115.1"
                     /translation="MKRLKNPVTFIFITVLVDCIGIGIIFPVIASLITEVSHVPVNKA
                     TTYSGWMMTLYALMQFIFSPVLGGISDRYGRRPVLLLSLLGLGIDYLFLSVAHTLTLL
                     FIGRIIAGICGASFTTSFAYIADVSPTEKRAQYFGMMGAAFGVGFIIGPVIGALASHW
                     GVRAPFVVAAFLSLLNFLYGYFILPESLKPENRRAFQWKRANPLGAFIQIKKSKNIRT
                     LIFTMFLLYLAGQVMPSIWAFYTKFTYNWSDAEIGYSLAFVGVMVAIVQGGLIKWSQT
                     KLGSHTSIYTGLSFYLVGLTLFAIANQSWMLYAFTFIYALGGIGPPSIQGIISGLVPP
                     NEQGELQGMMTALMSISTILSPLLMTNLFYNFTKPDTIYYFPGAPFAAAAILVLISFF
                     VVKRGINNLTRHRA"
     gene            complement(33460..34470)
                     /locus_tag="PKY09_04335"
     CDS             complement(33460..34470)
                     /locus_tag="PKY09_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNB12116.1"
                     /translation="MAVLGKKSKILNLIAVAAFLIISSCKQPVKESTETKQETPAKTN
                     VPVPAFNADSALVYLQAQVAFGPRVPGTDAHAKCADYLYQKLKSFGLQTQIQSETITT
                     FDSKKFRLKNIIAEYKPEAKQRVLLLAHWDTRPWADHDSVRKDQPFDGANDGASGVAV
                     LLEIARLLPASGLETGIDILFVDLEDYGQEEDDTRFPRQENTWCLGSQFWAKNMHRPD
                     YFAQYGILLDMVGGVNPVFPKEGTSLYYAPDVLSKVWNIAQKMGYGQYFINEPAPQTI
                     DDHLYINQLANIKTIDIVHYDNVKKEYPPYHHTHGDNLSIIDKNTLNMVGRVVLQTVF
                     NK"
     gene            complement(34445..35917)
                     /gene="cysS"
                     /locus_tag="PKY09_04340"
     CDS             complement(34445..35917)
                     /gene="cysS"
                     /locus_tag="PKY09_04340"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNB12117.1"
                     /translation="MEFQPLNIYNTLSRQKERFEPLNPPFVGLYVCGPTVYSDVHLGN
                     CRTFISFDLIYRYLLHLGYKVRYVRNITDAGHLEGDRDEGDDKFAKKARLEQLEPMEI
                     VQRYTLGFHDVMRAFNTLPPSIEPTATGHIIEQIEMIKQIISNGYAYEVNGTVYFDVE
                     KYSKEFNYGQLTNRLLEDMIANTRELGGQDEKKGRLDFALWINAKPEHIMRWPSPWGV
                     GFPGWHIECSAMSTKYLGATFDIHGGGMDLAATHHTNEIAQSQACSNVQPCRFWMHTN
                     MLTVNGTRMSKSAGNGFLPGELFTGNHPLLEKGYSPMAVRFFMMQTHYRSTLDFSNEA
                     LQAAEKGYQRLMSAIKTLNSVKASDVTSVNLSSIRKNAYDAMNDDFNSPVMVAQLFEL
                     VRIINSANDGKEKMTKEDIAEAKSIIQHFVFDIGGLMDDSVASGDQSMFDNVMQLVLE
                     MRNQAKAKKEFSVSDHIRNSLNAMGINVKDSKDGSTWEKK"
     gene            complement(35920..36879)
                     /locus_tag="PKY09_04345"
     CDS             complement(35920..36879)
                     /locus_tag="PKY09_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB12118.1"
                     /translation="MSQTERTLVLGSAGQIGTDLVEELRGIYGSDNVIATDIKDQPAD
                     FKAKGPFYMADVLDAQQVYDLIKKNNVKQVYLLAALLSAVAEQKPKAAWKLNMDGLFV
                     MLDYCKENTGIKLFWPSSIAVFGPTTPRKLTPQQTIMEPSTVYGISKQAGERWCEYYN
                     QKHQVDVRSIRYPGLISYKADPGGGTTDYAVNIFHDAVLKNTYDCFLKENTALPMMYM
                     PDAIRATLELMNAPAEKIKTRSGYNLAGISFSPVELVEEIKKHIPSFTCTYTPDSRQQ
                     IADSWPSNIDDSVAQKEWGWKHEYDLKEMTSEMLRHIRIKYNK"
     gene            37166..38830
                     /locus_tag="PKY09_04350"
     CDS             37166..38830
                     /locus_tag="PKY09_04350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB12119.1"
                     /translation="MKQLVIYLCTLFPIAFNCQAQLFNSAKPEFTHADSLRGFLSPIR
                     SCYDVKYYDLSVALNIPDQTINGSNSIWFEVVSPTKIIQVDLTEKLTVASITDEHNNK
                     LNFSRDGNAIMISFPTMLNKDMHYKIKIDYFGKPVVGKMLPWDGGFKWTKDKDGLPWV
                     AVACQGTGASVWWPCKDHQSDEPDSMMINITVPKTLMDISNGRLRKKTPVGDDMMQYS
                     WFVSYPINTYNVTINVGSFVHFNDWHVNNNDTLTLDYYVKSYNLEKAKKQFEQVKPML
                     NCFEKYFGKYPFYRDGYKLVETPYLGMEHQSCIAYGNKYKTGYMGMDFSGIGLDFDYI
                     IIHETAHEWWGNNITTKDIADMWVHEGFGSYAEVLYTECLYGKAKALDYVNAQRKKVG
                     NKTPIIGPYDVNTEGSGDMYPKGSLLLNTIRQYIDNDSLFFSILMNIQKTFAYQTITS
                     AAIESLMTKQSGKNLTPIFDQYLRHAAIPVFEYEVVKDKPFQLRYRWKADVADFDLPV
                     RIDCNKENKRLNVTTQWQETTLAGFDSNCLIIRDDLGYFKVTKLNK"
     gene            complement(38881..>39423)
                     /locus_tag="PKY09_04355"
     CDS             complement(38881..>39423)
                     /locus_tag="PKY09_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12120.1"
                     /translation="GVLTNDSVQSVKAYPVNDVTTATDAGYGYSFDNNLIGYDWKSFN
                     GSTFVIEDSVAYFVIDKNNHKWKMVFTGFGGSANGNFYFDKYDMGPLSVLENNSIEAI
                     ALYPNPATGNSQLVINSDNATTVTVDVTDLNGRLVSSFDKKVNGLNNITIPSESFVKG
                     IYIVKINSGKQLQQLKLVVQ"
BASE COUNT        12515 a         7500 c         7105 g        12303 t
ORIGIN      
        1 aatgtgccgg tataaacacc ttggttcatg atattataaa tttttagttc agcatttttt
       61 ctcggaattc ctttgaacag aatggtaaaa ttgccggatg aagggtttgg ataaacactg
      121 aaatcgaaag gattatttac ttcggcaatg cctgtgccgt tgcctaccat gccaaggctg
      181 tctgtcttga tgatgtaact tcctgtggat gttgccccac aaataataaa tcctccatct
      241 ttggtttgcc gaacaaaata gccgtaatta tcaatgtttg ggttatacgt tcttgtccac
      301 agtgtgtcgc ctgtggcatt tgtctttatt aaatataaac ttctgccatt agaattgtcc
      361 gtattgcctg tcattatata cccaccatcg gtggtttggt ctatggagta aacaacctga
      421 tgaagaggat aaaaatactt ttttgtccaa agggtatcac ctgcagcatc tgttttgatt
      481 aaataaatat tattcttata cacagtagta tcataggtaa ttgccgcaat aataaaatta
      541 ctgtcagtag tttgtttcac atccatggca gcatcgcttg ttgatttatc ataggttctt
      601 gtccataagg tatcgccata gccattggtg cggattacat aaatatcact atactgggat
      661 gatgtggcac ctgcacctgc cataataaat ccgttatcat aggttttatc tatggctcta
      721 gcatcgctgc ttaatgtaga agaataatta tatgttttat ttgccgccac caatccgttg
      781 ctcagtacgg caataaaaaa aactctggga acaattggca tcgtatcttg tttaaagtaa
      841 cccgttagtc catatatagc attattaacc gaagccatgc tgttaaccat tgagggtatg
      901 gttgctccag taccaccact taaaactttt gtccaaagcg tatcaccatt tgcgtcaagt
      961 cttagaagcc agcttcttgc aaaaggtacg cctatatcat aaactgcatt taccatataa
     1021 ccactatccg gagtttgctc aatataatta gccccttcca tactgggccc actgtaaatc
     1081 tttgcccatt caatggtgcc ggcagaatcg agttttacta tacaaacatc gttgccattg
     1141 tagatgctgc ctccgcaaaa aacatatccc ccatcaaagg tttcctgaat gctatatgcc
     1201 ccggcactcc ccaacgtatc aataattttt tcaaaagtgt tttgggcttt agctttcagg
     1261 ctgctcagca acactattat taatataatc tttatctgtt tctgcatagc gtctttatgg
     1321 tgtttggttt ttttggtgat atatacatta ataggcatcc tgccttttat cttctatagg
     1381 tatcacaaat gtaatcagta aatggtgttc ataaaaatat cttttggctc attttatttg
     1441 agcctttcgg ctcacattgc tcaaactaca ttatgaaaaa tagcaattat taaaaaaacc
     1501 taaccgaaat cataaatttt ctttatgaaa actatgtgct gaacccctga aaaaaaagag
     1561 gttgctgtat ttaaacaaca acctctttat tatcgttact gaaaaattta tctcgaatac
     1621 tgtgcttcca actgttcgaa tcgtttttgc aaatcgtcag ttatggcttt ctggtcttta
     1681 agtttcgaaa gcctgattaa ctcctgaaac actgccagtg cctgattggt ttcacgctcc
     1741 acgcttttgg cataaggtgt tcctttcaac gaaagataat attgcaaatc gttctgataa
     1801 atatcggcca aacgtttaac aattgtattc cctttttcaa tactctcttt tgaatctgtt
     1861 ttctttaatt ccacatcatt gctaagcatc tgcaagctgt caggcaatac attattcatt
     1921 gtgctgcgat agtacaactc ggcaatacgc aacatcataa tgttataagg aactgtttta
     1981 tcgggcattt ctaccagaca tttatcaaga actttcactg cagaatcagt ctcgttttct
     2041 gcaagcaatg tttcactaag acgtgcaaaa ttattacgga agttcatggt catgttcagg
     2101 ttgttctgat cgagataaac tctttcgtct ttcatattac cccacataaa tttattcatc
     2161 atgttgtcgt acatgcgttc cgcagcaaca ctaccggttt gccctgatgg gtcggcagca
     2221 gctttaatag gaacaaaacg ataagccaat ccttctgtct gaaaataatt ttcgagattc
     2281 agataggagt cgttacccac cgtcactgca aaatatattg ggcgtgtcca gtcgttgtga
     2341 gcaattaaat ccaaaatcat caaatctgct ttcatgatat agtttttgct gatttcaaat
     2401 tcaatataat caacaatctt gctggtatct tttgcctcca caacatgatt ttttatcact
     2461 gcctctttat ccacttttat tctgaacttt tttgtaggca gataattaaa ttcatcgcca
     2521 ccttcgctgc gcaccttagc ctcgggatta tcgctggtaa caaactcaat aatatctttc
     2581 aggtcgctat agccttgtac acttctgtcg taaaacggaa tataatcacg cgttccctga
     2641 acatactgtc ttgtttttag tgtaaatgga accggatctg attcataagc cttacgtttt
     2701 atctgatcta tgtaccagtc ggtgttgagc agactcaggt tgataacacg cacatctgta
     2761 cgcacacctt caacatcctg tatataccac aacgggaatg tatcgttatc accattggta
     2821 aaaatcactg cattgggtgc acaggaattg aggtagttgt atgcaaaatc gcgtgatgta
     2881 taacggtaag agcgattgtg atcatcccat ccttctttag ccataaccgt aggaacaaca
     2941 agacaaagca acgtagccag cattgccgca gtagtttccg gcattcgctt ctttaacgca
     3001 tcggcaattg ctgccacacc caaacctatc cagatacaaa atgcatagaa cgctcctgca
     3061 taagcataat cacgttcgcg gggctgatag ggtgtttgat tcaggtaaat cacaattgca
     3121 atacctgtca tgatgaaaag caacatcaca acattggcat tgttcatgtc ttttttatag
     3181 tgataaacca atcctgcaag accaagcaac aatggcaaaa agtaaaattt atttcttgct
     3241 ttattatttt tcatgctgtc gggtagtttg tcctgcggac ctaaaaacat actatcaata
     3301 aatggtatgc cacttatcca gtttcctttt agtatgctgc catgcccctg aatatcgttc
     3361 tgacgaccta caaagttcca cataaaataa cgccagaaca tgtggttcat ctgatagctg
     3421 aaaaaatatt taagattatt actgaactca ggtttctgtt caccttttat tcccgaccat
     3481 tctttgtatg ccgaaacatg gttaggctca cgactgtaca ttcttggaaa aactgtacat
     3541 cgtgcaggat catattcggg aataaccttg gctcctgtcg gaatatattt tccatcaata
     3601 gcagtatagc tcattgcacc ttctgtctgg cctacaactt tagcatcgta gtattgccca
     3661 taacctaacg gtctgtcgcc atactgctcg cggttgaggt agcctaatag cgcaaatgca
     3721 ttctccggat tgttttcatc cattggcgga ttggctaaag agcgtactac aatctgcgca
     3781 taggaagtat atcccaacaa cagaaatgca aaactcagca aaacagtgtt gagcagtgct
     3841 ttttgttttt tctgagaata gtaaatgcca taagtcaatg ccgctaaaat gatgagtgag
     3901 taaacaacaa atcctgtcca caaggatgaa cctgactgat tgaaccataa atcaattttt
     3961 gcacctatgt ttacaaaccc tgcaataatt ccatactgta caaccattaa aattaccaat
     4021 gatataccca ttgttttggc aacacccatc cacgaaaaag gatatttgcg gaagtaatac
     4081 accaatgcca atgcaggaat acaaagtaaa ttcagcaagt gaacaccaat agacaggccc
     4141 atgaggtagg ctatgagaat aatccagcgg atactgtgtt tttcgtctgc aacattttcc
     4201 catttaaaaa cagcccaaac aacaatacag gtaaagaact gtgatgttgc ataaacctcg
     4261 ccttcaacag cggaaaacca gaatgaatct gtgaaagtaa atgccatagc acccacaaca
     4321 ccacttgcaa ctacggttaa aatctgtgct aatgaaggct cctgattttt tacaaaaaca
     4381 atttttcttg caatggcagt aattgtccag aaggtaaaca aaacagcaaa ggcactcata
     4441 agggcgctaa aggcattaac catgatagga atggattgca aatcgctacc tgcaaaaaga
     4501 gtcataatgc gtgagagcat cagaaacaaa ggtgctccgg gtgggtgacc tatctgcaat
     4561 ttgtaggctg cggcaataaa ctcgccacag tcccaaaagc tgcctgtcat ttcaatggtt
     4621 gacagaaata cccacgctgc aatggcaaaa actgcccagc ctgaaatgtt gttgaatttt
     4681 gtatattgtt tcatgtaaaa caaagaaggt tacaagcgtg cgaaaatagc aaattatgct
     4741 tctgaatggc ttttaatttt aaataagctt tgaatacctt taacaacatt taagttatga
     4801 catgatttac tgatgaatat tcaaataact ataacgcttc ttctctattt aaaactaggc
     4861 gtttgtaaaa tgccatgtaa tacggtaaca ttgcaaaatg aacttagtaa ttgatatagg
     4921 caacacaaga ataaaagcag cagtgtttga tggtaaaaaa ttaattgcct gtcatatcat
     4981 taagtctttc aaagaaagtt cttttactgc attgataaac cgctatgcaa ttcgtgctgt
     5041 aattttttca tcagtagtaa aagaagaaaa aatattagga gcatctgaaa ttttacaggt
     5101 attttattta aaccataata cagcattacc catagttaat gattatgcca cacctgcaac
     5161 attaggcaca gaccgtttag ccaatgcctg tggggcagca ttgctcttta agaataaaaa
     5221 ttgcctgact ttagatgcag gtacttgttt gaagtttgat ttaatcactg ctgatgcaca
     5281 ctataaagga ggagcaattt ctcccggctt aaatatgcgc tacaaagcat tgcacgacta
     5341 cacaggcagg ctacctttag taaagcaaac cgataatgtt gcactgacag gaaacactac
     5401 ccaaacctct attgccagtg gcgttcaaaa cggtattatt gccgaagccg aatacctgat
     5461 tgctgaatat aaaaagcttt acaaaggcct gaaagtggtt gttaccggtg gcgatatggc
     5521 gttttttgca gataggctga aaacacacat ctttgcagcc ccggatttaa cccttgttgg
     5581 gttaaacagt attttgaact ataatctgag ccggcagtaa aacaagttaa taatgcagaa
     5641 acaaaaatat ttatttacag tacttttttt ggctttaggt cttacattgc atgcacaaac
     5701 gggaacagaa acaccctatt cgcgatatgg cattggcgaa ttactgaata gtggctttgc
     5761 ccgacagtcg gcaatgggcg gactgggagc agcctttcag agttcatctg ccgttaactt
     5821 cagtaatccg gcttcgtatt catctattcg cataagcact tttgaaactg cactgcgtgg
     5881 tgagcttgct caaacatcaa catcagattt aaaacagaca aaaaaaggca ttttactcaa
     5941 ctatctttct ttaggttttc cggtgatgaa agacaactgg ggtgccagtt ttgggttgat
     6001 acctgtaagt tccacaggct acaatctcaa agaatcaggt caggtaatta accctaacga
     6061 taccattaca acagatttca ctaaatatta tgaaggtaat ggcggattga gtcgttttta
     6121 cattggcaac ggatttgcac cttactcaaa aacgctcgat aaatattatg cttcgcaaca
     6181 ttataaagac ttaaaggaaa aaggcgacac agtcaccatt agtaaagaag tgcgatggag
     6241 aaaaattgtc aaaggatttt cagcaggttt taatgcatct tatctttttg gaagtcttaa
     6301 taacgtgcgt agggtaaagt ttagcaattc agcatttatc aatacaaaag ttactgaaag
     6361 cactgcctat gcagattttt attttgacta cggtattcaa tatcgctatg atttcagtga
     6421 agacaataaa cttatcatag gcatttcagg tgcaggagga acaaacatta aaggaagacg
     6481 tgatgtgctg tggacattgg tttcttcatc agattttgaa gcaaatattg acactgtttc
     6541 tttatctgaa aacgaaaaag ggaaagcatt tattccctca tttatcaatg cagggtttat
     6601 gtttcagcaa ggtgaaaagt ggctgatggg tgctgaatat gcaacgcagc aatggagcag
     6661 atacaaagct ttcaactcca atcaaaaact taaagattcg tggaggattt ctgcaggtgc
     6721 acaatacatg cctacaaata aattagccta ccgtgcagga ataaaagttg agcaatcgta
     6781 catcaacctc aataacagcc gagttaatga ttactcaatt tctttgggtg taggtgtgcc
     6841 tgtgcgtctt acctcaacag aaagaagatt gtttgataac cgcaccatgc ttcactttag
     6901 cattgaaggc ggacagttgg gtactacaag caataacctt gttaggcaac aatatgtgag
     6961 actttatgtt ggaattactg ccaacgagct ttggtttatc aagcggaagt acgattaacg
     7021 gaacgttaaa tcagcatatt aatcagattt ttttagtttt acttcagccg ccacccttac
     7081 actaaggtga gggtacttat atttgctgac agaaaacaaa atgaacaagc gctggacact
     7141 gctgaaacag gcagataatg ataagtcaat aaagtttggt aaagagttaa atattaaccc
     7201 tgtgcttgcg aacttattga ttcagcgtag tgtaaacacc tttgacgagg caaaagagtt
     7261 cttcagaccc tcattgacac tacttcacga tccatttctg atgacagata tggataaagc
     7321 ggtagcccgg atagataaag caattgctaa aggtgaaaaa gtattgattt atggtgatta
     7381 tgatgttgat ggcaccacag cagtagcatt ggtatatcgt ttttttaaaa atcgctttgc
     7441 cgaatgcaac ttttatatcc ctgacagata tactgaagga tatggaattt ctttgcaggg
     7501 aatagattgg gcaaaagaaa acggcttttc gttaatcata gcgcttgact gcggcataaa
     7561 agctatggat aaaacaaagt atgcatcaga attgggtata gattttatta tctgcgatca
     7621 tcacagaccg ggagcagaaa tcccacaagc tgctgctgtt ctcgacccca aacgtgtaga
     7681 ttgtgcatat ccatgcaaag acctttcagg atgcggagtt ggttttaagt tgcttcaggc
     7741 atttgctaaa aaaaataata ttgacgagca acaagtgctg gaccttattg accttgttgc
     7801 cataagtatt gctgctgata tagttcctat tcttggtgag aacagaatct tagcgtatta
     7861 tggtcttgag cttatcaata aaaatccacg tacaggaata aaagctattc ttgagttaaa
     7921 tgctatcaga cgcgaactca acattacaga tgtggtgttt atcattgcgc cacgcattaa
     7981 tgcagcagga agaatggatc atggcaggtc ggccgtacaa ctgctcatca gcgattctgt
     8041 tggtgatgcc ataacggaga gtgaaaaaat caataaaaac aatattgaca gacgcgatgc
     8101 cgacaagcta attacagaac atgccttgca gcagattagc cgcgataata cttttgaaag
     8161 caaacgaagt actgttgttt acagtgctga gtggaataaa ggtgtgatag gcattgtagc
     8221 ttcacgactt acagaaaaat tttaccgccc tacagttgtg cttaccgaca gcaacggaca
     8281 tattgcaggc tctgcacgct ctgtaaaaga ttttgatgtg tataatgcta ttgaagcatg
     8341 cagtgaatat ttagatcagt ttggcggaca catgtatgca gccggactaa cattaaagca
     8401 tgaaaatttt gaagccttta aacaaaagtt tgaagaagtg gtatgtgcaa ctattgaaga
     8461 gcacatgctt acaccggaga ttgaaattga tgcagagtta aaattagaaa gtattaccaa
     8521 accattctat aatgtgctga aacaatttgc accttttggt ccaggtaata tgtcgcccgt
     8581 atttgtcagc cgcaattgca ttgccgacaa aaatcaaaca cgaccagttg gtcaggaaaa
     8641 taaacatctg aaacttgcct tcacgcaaga tggatttgga aagaattttt ctgccatagg
     8701 ttttcagtta ggcgaatatg ccgacttact caagtcagga aaacgctttg atgtttgtta
     8761 ccatattgaa gagaatcatt ttaatggtaa cacagagttg cagcttaata ttaaggatat
     8821 aaaaatttca gaatagtttt ttctttacct tctgtggcgg tgggattttc ttttttatct
     8881 caatagaaat ttcaacacta tcaccaatgg ttttaccaat agcttcgcgc cattcttttt
     8941 ttattgtcag ccaatgtgag ccatctgttt tcggttgtaa ggcaccttca ataggatgat
     9001 tgtctataaa tcctactacc tcaataattc ccgtttcctt aaaatgatca cttgcttctc
     9061 tcaacaccac ataggtagaa acacctctta tgtctctgcg atggagattt gtttttacac
     9121 taaacttttt cgccatagca tttctgttta aataatttct ctataaagat aagaaaataa
     9181 aaatgaaaaa ctccggtaca aaaaattata ccggagtttt aacgttgaat aaattattaa
     9241 atcaggcttg ttcactgcgg ataatgttta atgcacctcc tgctttgaac caggcaatct
     9301 gatgttcgtt gtaagaatga tttactttga tttcttcttt tgatccatca gaatgatgca
     9361 ataccaatgt taatggaaca ccgggtgtga atgttgtcaa accaacaata tcaaaagtat
     9421 catcttcttt aattttctca taatctgctt tatcggcaaa tgtcaatgca agcatacctt
     9481 gcttcttaag attggtttcg tgaatgcggg caaaagattt taccagtaca gcccgtacac
     9541 ctaagaaacg aggttccata gctgcatgtt cgcgcgatga gccttctcca taattttcat
     9601 cacccacaac aattgaacct attccttttg ccttataagc acgttgtact ttaggcactt
     9661 catcatagct gccgtcaagt tgattcttta cactgttggt attatcatta aatgcattta
     9721 ctgcgccaat gagcatgttg ttggaaatgt tatccagatg tcctctgtat ttcaaccacg
     9781 gacctgccat tgaaatatga tcggtagtac atttaccttt tgctttgata agaagtttta
     9841 agccttttag gtctgacttt tcccatgcag caaatggtgc aagcaactgt aagcggtcgc
     9901 ttgtaggcga cacgattaca tcaactttac ttccatcttt tgccggtgcc tgaaagcctg
     9961 catcttcaac agcaaatcct ttaattggaa gttcaatacc ttgtggttcg gccaacttta
    10021 ctttttcccc attatcgttt gtgataaaat cagtaaccgg attaaaagta aggtcacctg
    10081 caatggctaa tgcagttaca atctctggtg aagctacaaa agcatgtgta ttggggttac
    10141 catcatttct ttttgcaaag ttgcggttga aagatgtgat gatagaattt tttttattgg
    10201 gatcgttggt atggcgagcc cactgaccaa tgcaagggcc acaggcattg gctaaaacca
    10261 cacctccaat tttttcgaaa ataccaaggt agccatcgcg ttcaacagta tatcgcacct
    10321 gctcgcttcc cggtgtaatg gtaaattcac tttttgcttt aattcctttt tcggctgctt
    10381 gtttagctaa tgaagcagca cgcgtaatat cttcgtaaga tgagttggta caagaaccga
    10441 taagtcctac ctctaattta gcaggccatc cgttttcttt cactgctgca gcaaacttgc
    10501 taagcggcca tgctaaatcc ggagtaaaag gaccattgat gtgaggctct aattcagaga
    10561 ggttaatctc tatcacttcg tcaaaatatt tttcgggttg ctgatatact tctgaatcac
    10621 cacgcaggtg agctgcaata ccatcagcaa ggtctgcaac atcagcacgt ccggttccac
    10681 gcagatagtc agccattttt ctatcgtaat taaaaataga tgttgtagct cctatctctg
    10741 cgcccatgtt acagatagtt cctttgcctg tacatgacat actctcagca ccttcaccaa
    10801 aatattcaac tatcttatct gttccgcctt taacagttag tattcctgct acttttaata
    10861 tcacatcttt agcagatgtc catccactta acttacctgt aagttttaca ccaataagtt
    10921 ttggcatttt cagttcccat ggtaatcctg ccataacatc acaggcatcg gcaccaccaa
    10981 caccaatggc aatcattcct aacccaccgg catttactgt atggctgtcg gttccaatca
    11041 tcattcctcc ggggaaagca tagttttcta aaattacctg atggataatt cctgcacccg
    11101 gtttccaaaa acctatccca tatttatttg agaccgatgc caagaaatca aaaacctcct
    11161 tacttttatc taaggcagag gctaaatctt ttgctgcccc gtctttcgcc tgaatcaagt
    11221 ggtcgcaatg cactgttgac ggcactgcaa cttggggtct gccggcttgc ataaactgca
    11281 acaatgccat ttgtgcagtg gcatcctgca tagctactct gtcggggttg aaatctacat
    11341 aggccttgcc acgttcatag ttttttacct caaactgtgg cgaaagatga ctatacaata
    11401 ttttctctgt taatgttagg ggacggttca gttttttacg ggcctgctca atgcgtgcag
    11461 gcatttcaac ataaaccttt ttaatcattt ctatatcaaa tgccatgatc aatgtttttt
    11521 attttatcgg aacgcaaaaa tacagataaa gatgattcat aattgttgac tgagtctaaa
    11581 taaccacctt agctgttttc aaaaaacagc tctcatcaaa aagagtttca atttttttga
    11641 aaaaaatttt ctttttaaaa agaatccatt acatttgggt accaaaaaaa taaatatcat
    11701 gaaaacacta caaaaacaaa tcatcactat ttgcatttta ctatcgctta caggcatcgt
    11761 atttgtatct tgcaagaaag acaaagataa caacactgct gctccggcaa cagaatatgg
    11821 tgttatcacc ggtaaagtac attcgcccaa aaatgttgcg atagccagtg ctaaaatcat
    11881 ttcaggcgaa tactccacca taagtaataa acagggggag ttttcactta gtttacctgt
    11941 tggatttcac aatcttataa ttcaaaccgg aagcgggcat gtattcaaaa ccgttacaac
    12001 agtgcaggtt aataccaatg aaaccattca tctggatgat acacaaacag aactgcaaca
    12061 aataaaacaa attgcattta ttccaggagt ttatgataga attgaaacta tcattataga
    12121 cacacttggc tataccgcaa ccgcaattac agttccctct cttgacaact tgaattttat
    12181 gcaaagtttt ggaggtatat ttttaaattg tggcgttagc ggaaatttag atagtttaaa
    12241 atatgccaat cttaacaatt atgtgattgg tggtggtaat atttatgctt ctgactggtc
    12301 agttgagttt ttaaccggtg atggtttttt cagaccttcc gcttcttcct tttttaccaa
    12361 cagtgcatcc aaaaatcaat cacatgaagc agcttatagt acaatgtcaa cctgtacctc
    12421 tcccttaatt ggcggatttt tacctgacag tgctttatgt actgccaaat atggacctgt
    12481 caccacggtg caaaatgcac atattcttga tccggatatt attaatgtat tgggtaaaga
    12541 ttctatggat atttattaca atttaaatgc atgggaagtc atcaacttag ttgacgcccc
    12601 atttaccacc atcattgaag atacgcattt cggattggga tcattagccg ttgcatgtga
    12661 tttaaacgga gccatgccgg caggacacat tacctacact acctttcaca atcatccaca
    12721 gggcaacatc agtcaggacg taaaattagt cttgcagcat tttattctga acatgtaatc
    12781 agcagcattt ttactgctta attaaaacaa ccaaaaaagt atttgggctg aatttgctgt
    12841 aagcaaactt attaagaacg cttcttttta cctttgccaa aatttagtgg caaatgagca
    12901 atacttcaga atatgatgtg gtagttattg gtggaggatg tgtaggcatg gcatcagcct
    12961 ataaacttaa cctgcgtttt ccacatctga aaattgcagt gcttgaaaaa gaagaccgtc
    13021 ttgctgcaca tcagacagga agaaactccg gtgttataca ttcaggactt tactataaac
    13081 ccggatctta taaagcaaaa aactgtgtag aaggcaggcg tgagctagtt gcattttgta
    13141 aagagcatgg tgtggcacat gatgtatgcg gaaaaattat tgttgccact gatgaaaaag
    13201 aattgccaca catgaaccgt gtttttaata atggtttgca aaatggcgtt gaaggaattg
    13261 aaaaaattaa tgcacaacaa ataaaagata ttgagccatt ttgtgcagga attgaaggaa
    13321 tctgggtgcc ctgcacaggc attgttgatt atgttgggtt aacaaataaa cttgccgaac
    13381 taattgaaca acaatttaca ggaagcaagg tttttaccgg tcatgaagta actgcattcg
    13441 taaagcaaga tggaaagaca acagtagtaa ccaataaaca aaaattcacc accaagcacc
    13501 ttatcagttg tacaggatta ttcagtgata gaatggccaa ggctgatggt gtaaatccta
    13561 atatgcagat tgtaggcttc agaggtgatt attatgattt gacagaaaaa ggtatgaata
    13621 aagtacgcaa ccttatttat cctgttccta atcctgagtt tcccttttta ggtgtgcatt
    13681 ttacaagaat gattcagcat ggtgtagagt gcggacccaa tgctgtgttt acttttaaac
    13741 gcgaaggcta tggaaagaca gattttgatt taaaagatac ccttcaggca ttagcatata
    13801 tcggcacatg gaaactcttt ttcaaacact ggcgttttgg tctcgatgaa tacaaaagag
    13861 ctttctctaa aaaattattt ttagaaagac ttcaacgact tattccttca ttagagatgg
    13921 atgatatcac tcccggacgt gctggtgttc gagcaatggc acttgatgcc aatggaaata
    13981 tgattgatga ttttaaaatt gaatacagcg gcaatgccat acacgttctt aatgcccctt
    14041 caccggcagc aacagcctca ttagctattg gaacagccgt gatggaaatg gcagaaaaac
    14101 attttcagat tcattaatgc aacataggtc atgccattaa aaaacattgg ctgccgctcg
    14161 ttggcaacat cgtacacttt aaaaataatg gcaataaatg tgactgcata attgtatgac
    14221 aacaataaaa aaaccacaag accaaccaaa caagttttat atcattagtg tgagtgtcct
    14281 tacttttgtt ttcccggcaa ttggtttttg ggcagaacat tttgccattg acactaccct
    14341 gtcgtttgac ttatttagca agtggtttat tttctctgct gttgggctac gtttgtttct
    14401 tgcaggtatc aaacaagtta tcaatcctga atttacagca aaacaaattt tccaaattga
    14461 cagtcccgat agctttccta ttcttcgcga attaggtttt gccaacattt gtttcggact
    14521 gactgctatt gtatcattgt ttaagcctga gtggcgcatt gtgagcgctt ttgccagcgg
    14581 actctattat ggttttgcaa gtatgcaaca cggattaaaa aaagctttgg gcatgaatga
    14641 aaaatttgcc ctgtggactg acttactaat ttttgttttt ctcctacttt actttataaa
    14701 agccctgtca gcataagacc aaactacaaa ataataattt aaaatctgct atggtatgag
    14761 gaaacatttt caggaaagaa aaatcgtagc ctcaaacatc ataaaatcat cattggtgca
    14821 gcaaattact catatcaaca aatgactatt atcatcacat ggcactacct gaaacttggt
    14881 caatttccta aatttgcagc agaataatca acaacgtaac atcatgcaat ccacaatcta
    14941 caaatttact tttttactga taagcattat attgtttaac acaggcagtc atgcacaagt
    15001 aagcactacc gacaccatga tggtttatgg caattgcaat atgtgtaaaa aaactattga
    15061 aggctcctta aaagagaagg atggcatcat tgcaaaaaac tggaataaag acactaaaat
    15121 gctgacagta acttatgatg cttctaaaat taataaagaa caaattgggc aaaaaattgc
    15181 agatgcaggc tatgacaatc agtatgcaac agctactgag aaagcgtata aaaaactgca
    15241 ttcatgctgt cagtacgacc ggcctaaaca accttccggt ggtgaaaaca aatagtcaca
    15301 caacaaacat aatacaatga aaaagcaact ccttacactt attcttttag ccatcacatc
    15361 tttaagtatg gcacaaacag caacagactt tacagcaaac gactgtaatg gcaactcaca
    15421 caatcttttt acagaattaa attccggaaa agtaattgtc ttatgttggg ttatgccatg
    15481 tgcatcatgt acagggccta cattaacaac ctataatgtt gttcagagtt ttcagtcttc
    15541 caatcctaat aaagtgttta tgtacacttg tgatgatttt gcaaacacca attgtacctc
    15601 tttaaacagt tgggcaaacg gaataggtgt tatgcaacaa agctggtcgt tgcggttttc
    15661 gaatgcagca attaacatga cacattatgg ttcaccggga atgccaaaga ttgtagtttt
    15721 aggacctaat cacgctgtat tctataattc aaacaatact gttaatgcaa ctaacctgca
    15781 aaacgctatt aacaatgctt tgacaacaac aggtttaaat gaaaataatt taccggcctc
    15841 atcattaact gttctgccaa accctgctca aaatgaaaca aaaattaaac ttaatcttct
    15901 gaataacgct gatgtaattc tggagtgcta taatcttgaa gggaaaaaag tacaggaaat
    15961 tttttccgga aacctgaata aaggagagca tactatttct gtaaatcttg aaccatttac
    16021 taatggcatg tatcttatca aattttctga cggaaaacaa tcacagttta ttaatctgat
    16081 aaaatccaac taatagaata actgtttttc agttggagta aattttaaat taaatatcta
    16141 tgcacaataa agttcaactg aagttttctt tagttctgtt attaatttgt ttctctgttt
    16201 ccgatgcctt ttcacaatgc tgtgctggtg gtagcggtag cccgattgcc ggtggaacat
    16261 cacaaggtgt tcttcaggaa aaacaagttg aaatcagcag taattttcaa tttatcagta
    16321 ccaataaatt ttatagaaaa aatatagttg cacccgactc tgttagaaca tttgatggct
    16381 ttaaaagtac ttatgaatat ttcagagtgg cttatggtat tacaaaaaat ttaacttttt
    16441 ctgttgagag tggctattat tatcaaaaga aagaaatagg tctgaataaa gatgcaacta
    16501 caacatacaa tgcaaaaggc tttggtgact taatactttt tccacgttat gatatcatta
    16561 actggacgga agaaaaacac cgtactgaag tgacagttgg tttaggtttt aaaattcctc
    16621 tcggaagtta taatgattca acaggcaatg tagagccatt ttcaggtaat acatattatg
    16681 ttaccaatcc tacttcggta cagctttcgt caggagcaca tgatattatt ttttacactt
    16741 tcctttatag agggtttact gaaaaaaatt tcagggtttt tgctaatgca ttatacatta
    16801 aaaaaggata taaccccaat ggagaaaaac ttggtgactt tgccagtgta ggcatttttg
    16861 caggtaaaac atttttcaat aacttaggtg taaccttgca ggcgcgatat gaatggatgg
    16921 acagaatgaa agttaatgaa tctatttatc tgttcggaaa accatcaaac tattttcctg
    16981 aagctacagg ctataaaaaa gtttttattt ccccacaact cagctttagc aaaggtaaat
    17041 ttactgttta tggctcatac gatattcctg tttaccagta tttaaataca tcggaacact
    17101 acacacaaat gggttcacaa catcaggcta ctattggcgt tactttccgc tttatggctt
    17161 tgaatagttt tctaaaagaa aaatcgggag ttacttatgt atgcccaatg catccgaatg
    17221 ttatttcaga taagcccggt aagtgtcctg attgtggtat ggacttggtt aagaagaaat
    17281 aattttaaaa ttacaacgaa aaagttccaa catacaatta cttggtctgt tttttttagt
    17341 aagaaagtgt ggaggttata acccatgctt tttgtgcaat ccgattctgt tatcagcatg
    17401 tattgctccc gtttaatata ctgcatgcgc ttaaagtaac agatgctttt tgggggcttt
    17461 gatgcatcat aaacaaaaaa gcctattgca gttatcagtt aacaaaaaat aatggctgct
    17521 ctcgattaga acagccatta tttcaattag tccttttatt tattttaatt gcttataata
    17581 aattttccgg agtaagattt tgcattaccc tccggtagca atctgaaata atataaacct
    17641 tctgcaagat ttttacgctc aataccaatt ctgttcattt tggtgctgga gcgtaaaatc
    17701 tcttttcctt ctgtgttata aacaacaaga tgatacgttt gattattttc attatcaaaa
    17761 acaatatcgg ccttactgtt aaatggattc ggtattactc taaccattga ctgcgatgaa
    17821 gcaggtattt ccgcaacaga agttggcaag ccaattacca tagtattgaa agtttcattg
    17881 gtaattatcg gatcattctg atcaaaatat attgctgcat cattaaatac agttgtgtta
    17941 tttggcaacc actgattggc cataatactg tatcgcacaa atccatgact tagcaactcg
    18001 ttggtgttac tgtcaaccaa cataatgtta gcaaatgtaa acaccacttc acggttgctt
    18061 agattaactt gtgtttgaac attatgactg gcatcaatca aggcaaatgt attcaggtca
    18121 agtccggtat caataacatc tctgacaacc actgtatagg ctgtatcatt tccggtgttc
    18181 tggaaacgta tcaaataatc caattgacta cccatgagtg tgtaatggtt gcttaggatt
    18241 ccggcaggtg tcactgattt atcattagga tcaaaactac aactaacaat tttacttgaa
    18301 attaatgtgt ccgtttccat cacagttccg tttatcaatg attccataat gatggtatct
    18361 gaaactacac tacccgctgc gggcattcca agttggaata tgatggagcc tgttgtctgt
    18421 gccgtcaatc ccggaaaatc ataaactact gtttgtccat tgacagacgt tggtgtaata
    18481 cttcctgcaa tgaaagtttg attggacgag aatttcatgg taattcgagc atcgtaagtt
    18541 acatatcctg tgtttttata attgactata gttgcattgt tcataagaca cctacgtaag
    18601 ccaacccgtg aaactacttt gcaatcataa accggtaagg tagagttaag accaaaatcg
    18661 agacctgtag tggtggctga agcatttaca ttgtagcttg attgactacc tgaagataaa
    18721 ctaaagccgg gaatagtatt ttgccactgt actgtatgag taccggcata acttcccaca
    18781 ctataatttc cggagttatc cgtaaacgca aatacattat caggctgcac gattactttc
    18841 tgacctgaaa agcccggttc tccgacatct ttaatgccat tactgtttgc atcataaaac
    18901 acattgcctt gcaaaaacag tgaaccttgg tctatataaa gcgcattttg taaatttaat
    18961 gttatgtaat taccaaaccc ggtaagatca taaactacac ccaggttgta ccaaccaata
    19021 ggtgcattgg caggaattga aaaatctgca taaaagtgta aatcgtctat aatattaaaa
    19081 gtatttgcat tggcatagta tgtaacagca gtatctgtac ggaataagta ggcatcatat
    19141 attgctcctg tgggtggtga tgaactttga aagacagtat ttgcacctga aatataggca
    19201 ctaaaaccag tacctgccag cgcagagaaa ggagacatgg aggtcaaata ctgatcggta
    19261 gtgttcaatg gaaaattgag ccctgttgta gtaacggaat agttaccact gatggtagcg
    19321 gcacccggtg gattgaccac tgtgttggtt gatggtgtga tggtaattgt cttattaccc
    19381 ggtgatgtcg gtattgtata gttacctaaa ttatcggttt gaacggtttg attgtcgggt
    19441 tgaagtgtaa tagtctgtcc tcccatgccg ggttcttgaa atcctaataa gccatccggg
    19501 ttgacatcaa caaatgcagt gcctgaaagg tacatgctgg ctgcataaac ggttacagca
    19561 ttggggaggg ttaaagtatg aataactcca agcgagtcac tatagtataa aactaaacta
    19621 taaacaccag gtgctaataa attaccactg ctattaaatg taactgaaaa ctgtgttgga
    19681 ttaattacag ttatattcga gccaccaact accggtgttc ctcctggagt tgtaagataa
    19741 gtgcttatta ttgcccctaa tggtgatcca ttttgcaaaa ccacacctgc acaagaaata
    19801 actgcattta aagtagtacc ttgaagcatt gaatctgccg agatgctgat aataacaggc
    19861 cctgttgttg ataatccaaa attatagcct gttaccggag ctgtaaaatt agccgtgtaa
    19921 gaagcctgac ttcctgctgt tataacgaaa cccgaagact gttgccacgt aatggttttt
    19981 attcccggat aagtgttaac catataagca ccgtttgaat tggtagtaaa tagagtattg
    20041 tcaggtgtta gaagtatttg ctgaccactc acaccttgct cacctgcatc ctgaatacca
    20101 ttaccattca gatcttcaaa gactacacct gaaatggtaa cgtttgccga aagaatttct
    20161 acggcattag gcaatgtcaa cgtaagatat gttccctgac tggctgtgtc ttcatagaca
    20221 taacgcaaat cataccaacc ataaggtgca ttgtagggaa tcacaaaatc aatctctgtc
    20281 tgtgtttcgg acaaagcatt catggaactc aatgacccaa taatactgtt tgttccctgc
    20341 tccaaataaa aacctgtcgt agaagaagac tgaaacaagg tgttgacccc gctgataaga
    20401 gccgataagg ttgccccccg ataagttgaa tccggtgata ccgatgttaa tgtttgcgct
    20461 aatgaggctt tgcacaacat tacaaaaacg agtactgcta ctaacttttt catgatttgc
    20521 gatttttagt aattaatatt tttgatagaa tctatttcat gtctgattac ctgaacaaaa
    20581 cttttgtttt gcttcatggc tatactttca taataccaaa gattggtccc tgtatatttt
    20641 attttccaaa ccggatttac atttggatta aacccaacag aacggaatat gctatcgcct
    20701 acatgttgta taacaaccat catgtagtct gcatacttag cgggaggatg tagctttctt
    20761 acttctctga ataaattata ggctcccaat ttgtttcctt tattaaacac cgctatggcc
    20821 tgattgaact tggcatccgg aaaacgtgga ttgatggcaa gtgctttatt aaaatattta
    20881 ttggcattgt cgttatcacc gttattcata taaacagcgc caatatcgtt cagaatttgt
    20941 acatgatagg gtgccatagc aatggcttct tcgtagtagc ttaaggcctc cttgggttgg
    21001 ttattcataa acgctgcagc tcctttatac catgcaatgg gcgtgccgtt ataatctatc
    21061 gggaaatagg tttcatccat tgacttggaa attctatagg ctatgggcca tttatttttt
    21121 tctttggcag caagcaattg catgtactgc gactctgcat accagcgtgc tgcagtaact
    21181 ttggttgagt acaatgcaaa aaccataaaa caaactatta ctgccggaac aagtttggac
    21241 atgactttct ttctgtcatc tttcatctgg taaaatacca ttaatgaaaa atacgcgata
    21301 acgcttatca tcaggagtag cttttccatt ggaaatccaa ataaagatac tgttgtaaaa
    21361 ataatcatcc cagacaatat aatcaaaaat tcttttgatt gatgggttaa atattgtttt
    21421 atagccatag taatccccca acccagcaat aaaatatagg taacaaatcc ggctatgccg
    21481 gtttcggaaa atatttgaag atagtcgtta tgtgccctgg tgaatttaat attatcagta
    21541 gtaagataat ctgcacctgt aaatccaaac ttaggggcca tcaatttcca gttagccaaa
    21601 ccacagcccg tccagaaatt ttctgcaatt agtttaattg aatttcgcca gagcatcatt
    21661 ctttcaaaaa cactgttggc attaaactca acatccgtag tgttgccttt gatataggat
    21721 actgcaatct ctgcttttct gaaaccatca attttattca gtactaaaat tactccactc
    21781 attgaagata ctatgatggc tatggtaacc aaattgatcc tttccggttt aatactcaac
    21841 ttttcagtaa ttacactttt aatttttgaa tactttagaa aggttagcac tatcataacc
    21901 aagacaagtg ctacaaaaac cgaaaaggat ttaagaatta tcaaatatac caatgataag
    21961 gcaagcgaaa ctattgctgc ttttttccac ctgtttgagt ctgtcagcat acacattact
    22021 tgcaatggca aacaaagtgc aatcatttca gcaaatgaat ttttattgga aagtaatgag
    22081 gtaagcctat aacttatttc aaatacttcc ttatgctcgt gtgcataatt ccatatacta
    22141 atcaactgca aaaccccata aatctgaatt ataaacagca gtactaccac ggttctggaa
    22201 atcattttca ttgggtctgc taccaatttg tttataatcg aaaacacaaa caaatagaac
    22261 aacaataata tccacttgat aaactcaaat aagccatcgt tgctacttaa actcttattc
    22321 caggctattc cggtgatgac tacaataccc aacaaaataa ataccggtgc aaaggaagca
    22381 ttgaatagag aaaaggaaat tttctttgta taaattccgt aaagaataag tgcagagaat
    22441 ggaaacacaa atactgctgc agttaacaat cttaaggtgt ggtcaggatc tggcaatgag
    22501 gatgaaaatg ataatgtcaa cactatcata aaaagtgcga ataaaaacga agcagctttc
    22561 tggattgaca tggccatcaa ttttaattat tttacctctc tacttaacac aaccctcaag
    22621 caaagataat tcttgacttt gaatattagt ataaatattc taattaaatg acagactttg
    22681 gaagcagcat gaattttcac tggctcaata aactaaatgc ctaaaactat gccttcttct
    22741 aaaaatccga cttttaatta aaaaaattac tcgtcaagtt gttaagtttg gatttaattg
    22801 tttgtatggt atttattgct tcactgtaag agctgttttt aaataaaaag ttgcaagtaa
    22861 cttaaaatat tctttttgta ttttaaaatc ctgaaatagt atctttacac cttcgagcac
    22921 atgaaatgaa cactgaatcc ctcatccttt ccatcagaaa aaatatcctt tggatactaa
    22981 tcattgcatt ggtagctttt gttgtcagtt ataaaatatc aggaagattg aacaactatt
    23041 acaaagctga tattatgttc ttgttaaacg atttaaatct aactgatgat tcgcccaagg
    23101 aaaacagcat tagtagtgtt atcaaaagca aatctttaga aactgaaagg attcttgcaa
    23161 tcatttactc tgatgcactg atacagaaag tagatgttaa actgaaactt ggaaagcatt
    23221 atcatttaga tgacaaagac ccaaaataca tgaccaaatt aaccgcttct tttgttaaaa
    23281 atgttactgt agaaataacc tccagtaaaa ttatgttact gaaagtacgt gatgcagaag
    23341 gacctgaatt tgttgccaac ctggctgaat ccattatgaa tgagcttttg gcttttaacc
    23401 gcgatcagac aatagaattg ttaaatgaaa aattagcttt taacagagcc ttgtttaaca
    23461 ccttggattc gcgctatatc agtaaattaa aaaatctgga acaatcagta gaacagctta
    23521 gacacaaccg tccaaaagaa atgactttta atgatttcct tattattaag tacaatatta
    23581 tggaaaccac taaacaaatg ggaatgttaa cttcagaaat tgaggataac atcaggagca
    23641 atcttttaat caataaaaca ttggaaggta aaaaactgga gagaataatt gtgttaaaca
    23701 aaaaatacct gatgccgcaa tcacaaaatt tgatgctgaa cctgtccgtt gcaaccattg
    23761 tatctatttt aaccatactt ttattaatag tggcgattca cagttacgga tccaacaaag
    23821 atttaatccg tctgtttatt tttggcaaaa agaaagaata gttttcatta atgttatttg
    23881 ttcaaccttc atttctaatc aggctttttt attcaggatt gatttggcga atgccaacac
    23941 ggcaacctga agtttacctg acatttgatg atggcccaga gcctgaagct acacctttcg
    24001 tactgtcgca gttaaagaaa tggaatgccc ttgcaacatt cttttgtatt ggaaataaca
    24061 ttgaaaaaca tcaggagttg tttaatcaaa tcgtttcagc aggacatcag attggaaatc
    24121 acacgcagaa tcatttgaat ggatggaata ataatcttaa tacttatatg gcagatgttg
    24181 aacaatgcag cacccatatt tcatccggtt tattcagacc accctatggt aaagttacgt
    24241 tgaaacaaat tcgctcacta cgttcacaat ggcagattat aatgtgggat gttttgtctt
    24301 acgactttga aacaaatgta tcaccagcaa agtgtattca gaatgtgatg caacatgtgc
    24361 gccccgggtc tatcattgtt tttcacgata gcaaaaaggc attaaaaaat ttaagtgaaa
    24421 cgctgcctgt tgttttggaa aagctttcag caaaaggata ctcttttaac gctatgcctc
    24481 cttaccggcc actacaataa caatttcgcc tttgggtgga tggttagtat aatactcagc
    24541 aacttctgct actgcacctc gctttttttc ttcaaacatt tttgaaattt cacgtaccac
    24601 acaaactttt ctgtcagcac caaaattggt gataaaatcc tgaagtgttt taataagctt
    24661 atgaggtgat tcgtaaaaaa ccatagttcg tatttcttcc tgaaggagtt taagacgtgt
    24721 ctgccttccc ttctttacag gtaaaaatcc ttcaaaaaca aaactgttac agggtattcc
    24781 agacattacc aaagcgggga caaatgctgt tgccccgggc aaacaagtga gttctatttc
    24841 atgttcaata caggcacgtg taagtaaaaa gccgggatct gaaatacccg gtgttccggc
    24901 atcactgatt aaagcaattg ttttttgttc ttcttttaaa agtcgaatca gttcattcac
    24961 cgctgcatgc tcattgtgct gatggtgtgt aaatactttc tttgaaattc cgaaatgatt
    25021 tagcaacacc gaagcatttc ttgtgtcttc agcaagaatg atgtcgcatg acttgagaac
    25081 atttaaagcc ctaagcgtaa tatcttccag gttaccaaca ggagtgggaa caatgtataa
    25141 cattcaccaa aagtaaccaa tagatgttaa ctttaatttt taataaactt tgtgttataa
    25201 acatgatcat ctgaagtcac gacttctaaa aaataaattc ctgctgacag ttgttcaacc
    25261 gttatccgtt ttaataaatc cactgaataa tttcttactt cttcaccggt aggttttaat
    25321 attcttacct ttttaaggcc aatatcggtt ggtaaattca ggtcaatata tttgtttgaa
    25381 gggttaggaa taacatcaag ataacctctg tcatattcat tcatccccac aaatggaaga
    25441 aataatacac aagctaaata aggattgtat ttaacaactg tgcctccctg tgtactgaca
    25501 ggccaaacca caactacatt actacctaat ttgtaattgg tactattgaa aaaatagggc
    25561 gactgaatta aaatgtttaa actgctatta ggttgtatgg tatatacagt tcctgaattg
    25621 agaattatag aatcttcagg gagtgaatcg ctctgaagtt tcaacatcag tccacctgaa
    25681 aaagttgaat tggcattata attcttaatg gtaacctgaa tattgatata aggttgttgc
    25741 ataaaagcag agtcagggca ttgtggatac tgtggtgaaa ttgcaataac ccctaatgaa
    25801 tctatttgtg ccgaagcaat gccacaaaac aacaaactaa taaacaacac tattatctga
    25861 acccttttca tgatgaagta ttttaaactt aagacttaat aaaagtataa attgtttttt
    25921 aatgctgaat atgtattttc gcattatctt acctgcccaa aagggactaa atggatattt
    25981 taaatcaact gattaacgga cttaacaaag aagaagtgcg tttttttaag atgtacatca
    26041 atcgtgttga gaagactgac aaccgtaaag atgtgcaatt gtttgattat atccgtaaat
    26101 cgggagatag attttcagat gaaattatct ttaaaaagct ctatcaacct caagaaaaaa
    26161 attcgtacta ccgtttaaaa aacagactca tcagagaggt aaataaaagt ctgacactac
    26221 agcattttga tgatgatatg gttgtatata tcttcaggct gctggcactg gtgaaatttt
    26281 atctgaataa aaatgcactg aaacctgcct atttttttct tcgcaaagct gagcaaaaag
    26341 ccaatgaaac agaaagctta gaattattag acattatata tggtgagtac atccgcctcg
    26401 cacgcgaaat gacaagtata aatccggaat tttacattca tcgcagaaaa gaaaattctg
    26461 agaaactcaa tcaactccgg gtcattgatg atattcttgc tgtagttacc tatcgcttaa
    26521 aaattactca aaatttatct tcaaaagacg aaacggtaat ttcactgctt cagaaaacta
    26581 ctgatgactt tatcggtgac ccaaagattg taaaaagtcc tctctttcgt ttcaggattt
    26641 acaatgcaat tagtcagttg ttgctgcaat cgcatcaata tacattatta gaagattacc
    26701 tgttaaaaac actaaaaata tttaatcagg aaaagctttt taccaaaaac aatcatgaaa
    26761 ccaaacttca actgttagtt tatttggtta atagtcttta taaaaataat aagctgaaaa
    26821 agtcgcttga atacacaaat atgttacatc aggagatgaa ggcttacaat caacttttct
    26881 acgataaatt tttattttat tactacaatg ctcttgtgat taactattcc aaaactgata
    26941 aacaaaaaga aatagtgaca ttgcaggaga tggagcaaaa tgaaaaaatt aatgctaact
    27001 tattctaccg tcagtttata ctattgaatt ttgcattggc ttattttgat cagaaagaat
    27061 ttaacctgtc ggcaaaaagt tttacccgat tacaaatgat gcctgattac aaagaaactg
    27121 atgagtcgct gcgaatgaaa gttgcagttg ctgaacttat gactcgtcat gagttaaacg
    27181 actacagcac ttttgattat aagttaaaac aatttaaaaa agatttcaga ggcgtcttga
    27241 ataaacctga aaacgaacca gaaaagctga tgagtgagat attaaaacta atgatgtctg
    27301 taaacaacag aaaaaacaga gccattgaat tgcggatagc acaactcaaa actttttcta
    27361 aagaagaaga tcaaagtgga ctgataaact atttagattg gcttcaggaa aaaaaattat
    27421 tcaagaataa aatcaggtag ttgcttcctt ttcaaggcgg tcaatatctg actgtattgc
    27481 agcaacaagt ttttcaaact gctctgcaca ttgctgcaat aattcagtag cagcattttt
    27541 ttcagttcca tcaagcatca gccattctat attttttaag attgatgcaa aggtgtctaa
    27601 gtcaagttgc ctgaatcccg gaatcattct gtgaacagct gctgccgatt gctgaaaatc
    27661 tgattcctta gcgaaatgaa tactcttttc aatcaagctt tgattactcg aaataaaaac
    27721 tttcaacatt ctgataacaa aatcgctttt cccatttgat gttctgaata aactatcgag
    27781 agaataactt agcaaaggtt tatgttgcac ctcacgattc ttctctatca gaggtataag
    27841 aacttcagtc agttcatgaa gcttaaatgg tttcaaaatg actgcattca tccctgcggc
    27901 tatacattcg ttttttgcat cattcatcat attggcagta atgccaatca aaggcaacat
    27961 agcacttata tgctcacgaa taaaagaagc agcattgtat ccatcaagtt caggcatctg
    28021 aatatccatt aacactccgt caggattata caacagaatt ttttcaattg cctgcttacc
    28081 actttcagca gttattactt ctgcaccgcc atctttcagc atggcagtaa ttaattgtct
    28141 gttaagcaaa tcatcttctg caacaagcaa acgaaatcca tgtaatgaaa caaactcttt
    28201 ttcagtaatt ttagtttcat tcattggggt caaaggaaaa cgtaacataa cggttactgt
    28261 agttcctttt tcgggaacac ttttcatgga tattgttccc ttctgtaact caaccaatct
    28321 ctttgtgatg gctaatccca gacccgttcc accatagttt ctttcaatat tatcggcttg
    28381 ttgataggca tcaaataatt ttgcaatctt ttcttcggca atgccaatac cggtgtctgt
    28441 tacctcaaac ttaaacaatt ccccttcctg tttttcaact tttactaata cggtgccttt
    28501 ctccgtaaac ttaattgcat taccaatgac attgatgagc acttgccgca aacgcaaggc
    28561 atcaccggaa accgtcaaat tacgaatatt actagcttct aaaactaatg caatattttt
    28621 ttcgtcagct ttttgactta ataaagttac tacatcattc acctccttat aaataacaaa
    28681 aggatcctgc ttaaaattta acttacctgt ttcaattctt gaaagatcta acacatcatt
    28741 tacaatattt aacaggtgtt gccctgccat gttgatagtg ttcacatact gctgttgctc
    28801 tgtagttgtg ttagtctttt gcagcctatt gataaaaccg gttaatgcac ttaatggtgt
    28861 tctgatttca tgactcattg ttgccagaaa ctcctcctta aatcgtgcaa gttgctctga
    28921 acgtgcccgc gcttcctcaa gctgaatttt atatctgttg gcacgactga tatctgcaag
    28981 aattattatt gaaagtatta atatgagaac tgaagctgta atagttgcta tcacaacagt
    29041 ttttaattgc tgttgcgaat tactgattac ttccaatgat ttgttttcat tgttatgcct
    29101 tatgtctcct tcaagtaaaa caatttgcga ctgaattctt tctttcaaca atctgtcttg
    29161 ttcaagtaat aacaactcac catcactcag ctgggtcacc tttctaatct cattctcctt
    29221 agcttcagaa acagtttgtt tgatagtact caacttctgt gcttgcaggt catttaaaga
    29281 atcaagcaac aaagcttttc tttcaagttc ccttaatttc ttttttgaag aaaataagct
    29341 tttaaaaaat ccgggctttg aagtttctgt actatctata ttctgataaa tttctacatc
    29401 ctcaacttta tcagaaattt tactcattgc agtattgata agttctctgt aacgcaattc
    29461 aatcaactta tcgtaaatgt caaacttata atgtatagat gctgtgagtg agtcaaaagc
    29521 ctgactgttt gacggaaaca gatttttaag ttttaccaat tgtgaatcgg tttgctcaac
    29581 ttgcttataa taatcttcta agtttttttc attgcgtgta atagcaaatg aacgcacatg
    29641 gctttctgca aggttaacat ttagaatcaa ttctttcagg tcagaaagtc ttgtatcagg
    29701 tttctgcaac agtgtaattg ttttgctaag tgctgttgta ttctgataat taaaccatgc
    29761 cagaaaaaat gctactgcca gtgcaagtaa catgaggaaa actatataca accgtttctg
    29821 cataatgagt caaaaataca acgctaaatc tcccaatgga ggaataattt tcccaaaatg
    29881 aaactattta tcagaaaata gactttcatt acttgcacgt taataacttt ataacttatt
    29941 gattggcata accttaaaat gtttacaaac caactgaatc aataagcgct aaggcagtgg
    30001 cacattattt aatagtctgt tgatgttaat caataaaaat aaaatacaat gaaaaagcta
    30061 attttaattg cattaccaat agtaggaatg accttatttg gttgcgaaaa ctataaagaa
    30121 caagctgcac agttaacacg tgagcgtgat tctttagtct cattgaatgt tgccggaaca
    30181 caaactattg atgagtttat cacaacattg actgacgtag aaaacagcct tggcaagatt
    30241 acagaaaaac aaaattctat tgcaatgact gctgaccgca atcctgaagc agcgcgcaac
    30301 tcacgtgaga gactcaacgc acagattgaa gaaatcaatc gtatgatgca ggagaacaaa
    30361 gataaagttg agcaattgac aaagaaatta aaaggtgcca acggatcagc agcaaaattt
    30421 cagaaaatgg ttaagtcgct tcaggagcaa cttactcaaa aagaacaaga gcttgctttg
    30481 ttaaatgaaa aattagcagg attgaatgtt gaactggcta cattaaaaac caacttcgat
    30541 tcgctgtcaa caacaaatca aaatcaggct actgttatag ctgaccaaac aactaaaatt
    30601 cacactgcat attacactgt aggaaattac aaaagcctgc gtgacaaaca agtgctttct
    30661 aaaaaaggtg gcgtgcttgg tttaggtaaa aaagcaacag ttgtgcctgc aattaaaaac
    30721 gataatttca ccaaaatcga ttatacccaa atcagcacta ttcctgtgaa tagtaaaaaa
    30781 gttgagttgg taactacaca ccctgatgga tcatacaaaa ttgaaaagaa caatgacctt
    30841 gttagcgaca ttttgattac agatcctgaa aaattctgga gtgcatcaaa atatcttgta
    30901 gtactgacaa aataataaaa acaggaaata aaacattctt tggttttagg ttgggtaacg
    30961 gagcagcaat gctccgtttt tttttaataa atgaatgttt aataaaattt attacctctg
    31021 tttgctttgt agcaaagtca ttatacattt gcccatcctg acattacgaa gttatgctaa
    31081 agaaaatttt cttccttata ttattttcat tcggctttat agaactcttt gcacaaacag
    31141 agcctgcaaa agcaacagaa aaagaatcgt tagttaactg gatcagctta aacgaagccg
    31201 aaaaacgcaa caaagagatt ccaaagccca taataattga tttttatacc agttggtgtg
    31261 gctggtgtaa ggtgatgatg agctcaactt attcagaccc gtcaatagca tcgtatatca
    31321 ataccaattt ttatcctgtt aaatttgatg ctgaaactaa agacaccata gcgtatgatg
    31381 gtgttactta caaaccaaca ggtacacaaa agcgcgaccc gaatgaattt gcagtaaaaa
    31441 tgctgaatgg cactatggtg tatccttcaa ctgtttttat gaataagtct gctaacttca
    31501 ccatgtctgc acagggatat ttggaaacaa agaagattga acctatgttg gtgtttacat
    31561 tagaaaatgg gtttcgtaac agcagctatg aagactttaa tacacaattt caaaaagctt
    31621 tttatgattc attacaaacc aaggtttacg aatctgttaa atggcaaaca ccatcacatt
    31681 tttttataaa agaccaaaaa cctgatgaga aaaagaaaat tgtttttatc aatacagaat
    31741 ggtgcaatac atgtcgtgtt atgtacagaa caacttttag tgatactgcc gtaagtagtc
    31801 tgttatctaa acattttgaa ttgataaact tcaatccgga aaccaacgac aaactttatt
    31861 ttcaggataa agaattcgaa aacatacatt caaaagaaat gccttttcat cagttggtgt
    31921 atgcacttag ccgcaacgga ttaatatttc cacaagtgat atttatggat gaaaagaata
    31981 ctgttgttga cgcaattccg ttttatctta atccaaaagt ttttaaaagt attgtgcgtt
    32041 tttatggaga agatatttat aaaacaaaaa actgggagac ttttatcaaa gaacaggaga
    32101 caaaataatc ccgtataatt atccaacaca aaatttaaaa acctaataac acaaatcgtt
    32161 aatgtatgaa acgtttaaaa aatcctgtta cttttatttt cattactgtt ttagttgact
    32221 gtattggtat tggaattatt tttccggtaa ttgcatcact gataacagaa gtcagtcatg
    32281 ttcccgtaaa caaagcaaca acctacagcg gctggatgat gacactttat gctttgatgc
    32341 agtttatttt ttcgccagtc cttggaggta tcagtgatcg ctatggtcgc aggccggtgc
    32401 tgcttttgtc attgttagga ttaggtattg attacttgtt tttatcagta gcacatacgc
    32461 tcacacttct atttattgga agaattattg ctggaatatg tggtgcaagt tttacaacga
    32521 gttttgccta cattgccgat gttagtccaa ctgaaaagag agcacagtat tttggtatga
    32581 tgggtgcagc ctttggtgta ggttttatta ttggtcctgt tattggtgca ttagcaagcc
    32641 actggggtgt gcgtgcaccg tttgttgttg cagcattttt gtcgttgcta aattttctat
    32701 atggatattt tatcttgcct gaatcgttaa aacccgaaaa caggagagca tttcagtgga
    32761 aaagagccaa tccattaggt gcatttattc agattaagaa aagcaaaaat atcagaacgc
    32821 tcatctttac catgttttta ctttatcttg caggtcaggt aatgccatct atctgggcat
    32881 tctataccaa gtttacctac aactggtctg atgcagaaat aggttattca ttagcttttg
    32941 tcggtgtgat ggttgctatt gtacaaggcg gattaattaa atggtcacaa acaaaattgg
    33001 gtagtcacac ttcaatttat acaggacttt ctttttatct ggtaggatta acactgtttg
    33061 ccattgccaa tcaatcgtgg atgctttatg catttacttt tatttatgca ttaggaggca
    33121 ttggtccgcc atcaattcaa ggaattatat caggcttagt accacccaat gagcagggcg
    33181 aacttcaggg aatgatgact gcattaatga gtatttcaac aattttatca ccactcctta
    33241 tgacaaactt attttataat tttacgaaac cggatacgat atattatttt ccgggagcac
    33301 catttgcagc agctgcaatt ttggtgttga tttcattctt tgttgttaag aggggaataa
    33361 ataacttgac gagacatcgg gcataattaa ttgatgcttt tttctatcaa ttaatttaac
    33421 agtaatcaaa gttgaaacga tagctataag caaaaacatt tacttattga aaactgtttg
    33481 caacactacc cttccaacca tattaagtgt atttttatca atgatagaaa ggttgtcgcc
    33541 atgtgtatga tgatagggcg gatattcttt ctttacattg tcgtaatgaa caatgtcaat
    33601 ggttttgata ttggcaagtt gattaatgta taaatggtca tctattgttt gtggtgcagg
    33661 ctcattaatg aaatattgtc catagcccat tttctgtgct atgttccaaa ctttactcaa
    33721 cacatcaggt gcataataca atgaggttcc ttccttagga aaaaccggat taacaccacc
    33781 aaccatatcg agaagtattc catactgcgc aaaataatcc gggcggtgca tattctttgc
    33841 ccagaattgt gaacctaagc accatgtatt ttcctgtcgg ggaaaacgtg tatcatcttc
    33901 ttcctgccca taatcttcaa ggtcaacgaa caaaatatct attcctgttt caagacctga
    33961 tgcaggcaaa agtcttgcaa tttccagcaa tactgccaca ccacttgcac catcgtttgc
    34021 accatcaaaa ggttgatctt ttctaacaga gtcatgatcg gcccaggggc gtgtgtccca
    34081 atgtgcaagc aacaacactc tttgttttgc ctccggttta tattcagcaa taatattctt
    34141 cagtctgaat tttttactgt cgaatgtagt tattgtttca gattgaatct gtgtctgcaa
    34201 accaaacgat tttagtttct gataaagata gtcagcgcac tttgcatgcg catcggttcc
    34261 aggcacgcgc ggaccaaatg caacctgtgc ctgtaaataa accaaagcag aatcagcatt
    34321 aaatgcaggc acaggaacat ttgtttttgc aggtgtttcc tgtttcgttt ctgtgctttc
    34381 cttaacaggt tgtttacacg aacttatgat aagaaaagcc gcaacagcaa ttaagtttaa
    34441 aattttactt tttttcccaa gtactgccat ctttcgagtc tttaacattt attcccattg
    34501 catttaaact attacgaata tgatccgaaa cagaaaattc tttttttgct ttagcctgat
    34561 tacgcatttc aaggaccaat tgcattacat tatcaaacat agactgatcg cctgaagcaa
    34621 ctgaatcgtc catcagccct ccaatatcaa aaacaaaatg ctgaataatt gattttgctt
    34681 cggcaatatc ttctttagtc atcttctctt ttccatcatt ggcagagttg attatccgaa
    34741 ctaactcaaa caactgtgca accattaccg gactgttgaa gtcatcgttc atggcatcat
    34801 aggcgttctt tcttattgaa gataaattta cagaggtaac gtctgaggcc tttacgctgt
    34861 taagagtttt aatagcagac atcaatcgct gatagccttt ttctgctgcc tgcaatgctt
    34921 cattgctgaa gtcgagtgta ctcctgtaat gtgtctgcat cataaaaaag cggactgcca
    34981 taggagagta gcctttttca agtaaaggat gattgcctgt aaaaagttca ccgggaagaa
    35041 aaccattacc tgcagatttt gacatgcgtg tgccgttaac cgtaagcata ttggtgtgca
    35101 tccaaaagcg acatggttgc acgttgctgc aagcctgaga ctgtgcaatc tcattggtgt
    35161 gatgtgtagc tgctagatcc attccaccac catgtatatc aaatgtggct cccagatatt
    35221 ttgtgctcat tgcagaacat tcaatatgcc atcccggaaa gccaactccc cacggagaag
    35281 gccatcgcat aatatgttca ggcttggcat taatccaaag tgcaaaatcc aatcgtcctt
    35341 tcttctcatc ctgcccgcct aattcacgtg tgttggcaat catatcttcc agcaatcggt
    35401 tggtgagctg gccataatta aattctttgc tgtatttctc tacatcaaaa taaactgtgc
    35461 cattcacttc ataagcataa ccattgctga taatttgctt aatcatttca atctgttcaa
    35521 tgatatgtcc tgttgcagta ggctcaatgc ttggcggcaa ggtgttgaat gcgcgcatca
    35581 catcatgaaa acctaaagtg taacgctgca caatttccat tggctctaat tgttccaagc
    35641 gtgctttctt agcaaactta tcatcacctt catctctgtc gccctcgagg tgacctgcat
    35701 cggtaatgtt tcttacataa cgcactttat atccaagatg cagcagataa cgataaatga
    35761 gatcaaaaga aataaatgtg cggcagttgc ccaaatgcac gtcactgtaa acagtagggc
    35821 cgcaaacata cagtcctaca aatggcgggt ttagcggttc aaagcgttct ttctgtcgcg
    35881 aaagtgtatt gtaaatattc agcggttgaa attccatgtt tatttattgt attttattct
    35941 gatgtgcctg agcatttctg aggtcatttc tttgaggtcg tactcatgtt tccatcccca
    36001 ttctttttgt gcaacactgt catcaatatt tgaaggccat gagtcagcaa tctgctggcg
    36061 tgaatccggt gtgtatgtgc aggtaaaaga agggatatgc ttttttattt cttcaaccaa
    36121 ttcaacagga gaaaaagaaa tacctgcaag attatagcca ctgcgtgttt taattttttc
    36181 tgcaggagca ttcatcagtt ccaatgtggc acgaatagca tcaggcatat acatcatagg
    36241 cagtgcagta ttttctttca gaaaacagtc gtaagtattt tttaaaactg catcatgaaa
    36301 aatatttact gcatagtctg ttgttcctcc tccgggatca gctttgtaac ttatcagacc
    36361 gggatagcga atgcttctga catcaacctg atgtttttga ttgtaatatt cgcaccatcg
    36421 ctcaccggct tgcttactga taccatacac tgttgatggc tccatgatag tttgctgtgg
    36481 tgttaattta cgaggagttg tcggaccaaa aacagcaata gagcttggcc agaataattt
    36541 gatgcctgta ttttctttgc aatagtcgag cataacaaaa agtccgtcca tgtttagttt
    36601 ccatgctgct ttgggctttt gttctgccac tgccgacagc aatgctgcaa gcaaataaac
    36661 ctgttttaca ttgtttttct ttatcaggtc ataaacctgt tgtgcatcca acacatcggc
    36721 catataaaat ggaccctttg ctttaaaatc ggcaggctga tcttttatgt ctgttgcaat
    36781 aacattgtca gacccatata ttcctctgag ttcttctacc aggtctgttc caatctgacc
    36841 tgcagaaccc agcacaagcg ttcgctctgt ttgtgacata ttttacaaat atctgtgaaa
    36901 ggacaaaagt aaaatttctg ggcgtttgtt tatgcgttta ataaattaat aaaccacagt
    36961 ttaaaccatg gactttgtaa ctgcggttcc atctcgtatt gtcaacaata aattatcaac
    37021 tgtcactttg ccggaaacct cctaacgttt tagaacataa gtttagtggg ttttattctt
    37081 tgttctcaag aattaaaaaa tgcttttgca atttcttaac tatttattgg aatatgcttt
    37141 ctacttttga aaaaattcct gaacaatgaa acagttggta atttacttat gtacactttt
    37201 ccccattgcc tttaattgcc aggcacaact ttttaatagt gctaaacctg aatttactca
    37261 tgccgattct ttacgtggtt ttctgtcgcc tataagaagt tgttatgatg ttaaatatta
    37321 tgacttatct gttgctttaa atatccctga tcaaactatt aatggcagta acagcatttg
    37381 gtttgaagta gtaagcccga caaaaattat tcaggttgat ctcacagaaa agctaacagt
    37441 tgcttctatt actgatgaac ataataataa attaaacttt agtcgtgatg gtaatgccat
    37501 aatgatttct ttcccaacaa tgttaaataa agatatgcat tacaaaataa aaattgacta
    37561 tttcgggaag cctgttgttg gcaagatgct gccatgggat gggggcttta aatggactaa
    37621 agacaaagat ggtttacctt gggtggctgt tgcctgccag ggaacaggtg ctagtgtgtg
    37681 gtggccgtgt aaagatcatc aaagtgatga gcccgatagt atgatgataa atatcaccgt
    37741 tccaaaaacg ctgatggata tttcaaacgg aaggcttcgt aaaaaaactc ctgtgggtga
    37801 tgatatgatg cagtatagtt ggtttgtgag ctatccgata aacacttaca atgtaacaat
    37861 aaatgtaggt tcttttgttc attttaatga ttggcatgta aacaataacg acactttaac
    37921 gcttgactac tacgtaaaat catacaactt agagaaagct aagaaacaat ttgaacaagt
    37981 gaagcctatg ttgaattgtt ttgaaaaata ttttggtaaa tatccttttt accgcgatgg
    38041 ttataagttg gtagaaacac cttacttggg catggagcat caaagttgta ttgcctatgg
    38101 gaacaaatac aaaaccggat atatgggaat ggatttttcg ggtattggat tagattttga
    38161 ctacatcatc attcatgaga cagcgcatga gtggtgggga aataatataa cgacaaaaga
    38221 tattgctgat atgtgggtgc atgaaggctt tggcagttat gcagaagtgc tttatacaga
    38281 atgtttgtat ggaaaagcaa aagctcttga ttatgtgaat gcacaacgta aaaaagtagg
    38341 aaataaaact cccatcattg gcccctatga tgtaaataca gaaggcagtg gagacatgta
    38401 cccaaaagga agtttgttat taaacactat cagacaatat attgataatg actctttgtt
    38461 tttttctatt ctgatgaaca tacagaaaac ttttgcctat caaaccatca cttctgcagc
    38521 tattgaatcg ttgatgacta aacaatcagg taaaaatctt actcccatat ttgatcaata
    38581 cctgcgccat gcagcaattc ctgtttttga atatgaagta gtgaaagaca aaccatttca
    38641 actgcgctac cgctggaagg cagatgttgc ggattttgat ttacctgtaa gaatagattg
    38701 taataaagaa aacaaacgat tgaatgtaac tactcaatgg caggaaacaa cacttgccgg
    38761 gtttgacagt aattgtttaa tcattagaga tgatcttggc tattttaagg tcacaaagct
    38821 gaataaataa aacaggctgt ccgttaaaac agacagccat gtattaaaat caactcaaaa
    38881 ttattgtaca accaatttaa gttgttgcag ttgcttgccg ctgttaattt tcacaatata
    38941 gattcctttt acaaaacttt ctgaaggaat agttatattg ttcaatccgt ttactttctt
    39001 atcgaatgat gaaaccaaac ggccattcaa atctgtaacg tcaacagtaa ctgttgtggc
    39061 attgtcgctg ttaataacaa gttgcgagtt tcctgttgca ggattaggat aaagcgcaat
    39121 ggcttcaata ctgttgtttt ctaaaaccga taaaggaccc atatcatatt tatcgaaata
    39181 gaaattacca ttagcagagc cgccaaatcc ggtgaatacc attttccatt tatgattgtt
    39241 tttgtcaatg acaaagtagg ctacagagtc ttcaattacg aatgttgagc cgttgaatga
    39301 tttccagtca tagccaataa ggttgttgtc gaaagaataa ccataacctg catcggtagc
    39361 agtagtaaca tcatttacag gataagcttt aacagattgc acactatcgt tggtgagtac
    39421 acc
//
LOCUS       DAOQNP010000019        38051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_346_length_38051_cov_8.625118, whole genome shotgun sequence.
ACCESSION   DAOQNP010000019 DAOQNP010000000
VERSION     DAOQNP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 38051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38051
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_346_length_38051_cov_8.625118"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PKY09_04360"
     CDS             <1..1130
                     /locus_tag="PKY09_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12121.1"
                     /translation="RIWFETNGYGAVFTGSNYQGGNDLTPQSGMNEYGLSFGTLATPT
                     PDKVKSFVNKKPIQSRSKYLKDILHSCKNVEEVQAFIEEYDHSTFSNDVFIYTDKSGK
                     YLIVEPYELIPGNDNKYVLANFCPSTITDFGSIKQQRYVDGVAFLKDKIDTSLAFCTA
                     LSDTMHVCRKKIGDGTLLTSILDLNNGIVHLYFYHDYKHMVQFNLKEELLKGNHSYEI
                     ASLFPANIEYQKLLDFQTPLNNNTIDWFLRLCLILFVFSFFYFLISYIKNRKTELANY
                     KLLLASLSLCLAYYMFALATVMAIFYFPSPYKSYKFGLIDIASYLPYLLILLIIPLLI
                     INRKVFKVNTWKTTSKFLFTANNIAYLILISLFCYWGFYNI"
     gene            complement(1418..2368)
                     /locus_tag="PKY09_04365"
     CDS             complement(1418..2368)
                     /locus_tag="PKY09_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12122.1"
                     /translation="MKKGILMLSLAAGISLMAACKKDKSEDLLDEQTTQQVTADDDAR
                     IAASDNEELDDANRIIAMHPRLRGINFMGIYNGLVVPCNTTIDTSLISQGKVTVTFNG
                     LNCNGTRNRTGTMIIQLPYDANTSTVTPWSTAGSQLTLTLNNVEITNVSSGKSITINS
                     TRTITNVNGGLIDNSPNFTTPILHHATGNTMVTFDDGTTRSWNFDRNRLVERTNNVTT
                     ITITGNASQGGYSNVSVWGVNRAGNTFFVSTDTPIILSSDCNYNPMSGVRVHHGVVRE
                     LTVTFGVDQQGNAQTSGCPYGYKLNWTSANGQTHQIVRSY"
     gene            complement(2484..2984)
                     /locus_tag="PKY09_04370"
     CDS             complement(2484..2984)
                     /locus_tag="PKY09_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB12123.1"
                     /translation="MFIDKETQRLHLRPLNTNDTLFILELLNTDEWKEFIGERNIHNH
                     DAALKYIENILAHSVYYYHIIELKSDKKPVGIITLLNRDKQQFPDIGFAFLPQYTGYG
                     YAFEASSFYLNLLIKAKSYSKIIGVTKPDNFKSIKLLTKLGLKYEYDQLENDTLLSVY
                     SLKIPD"
     gene            complement(3095..3847)
                     /locus_tag="PKY09_04375"
     CDS             complement(3095..3847)
                     /locus_tag="PKY09_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017257772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB12124.1"
                     /translation="MIINKTTEEMDMRVFVPIERHKKLIQLFKELPVGESFIFINDHD
                     PIPLYYEFRSIYGDVVGWDYLNKGGREWKVKVTRTEASQGREFSDISTLLDLRKAAVA
                     DRKQIVFHRYGMMEQGSTMEIIAAEDPTEIHGIFIQKFEGKHRWIYKKQEDGEVVVHI
                     TKTESSGLGDDGFSVVEEFDLRPFPPATRHEMFYDAFAAINPGEAFEFINDHDPLPLY
                     YQMEAESEVPFKWEYVLKGPEEWKVRVRKVKE"
     gene            4048..6108
                     /locus_tag="PKY09_04380"
     CDS             4048..6108
                     /locus_tag="PKY09_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNB12125.1"
                     /translation="MVISQLIQPKSIAVIGASDNITKPGGMVLQNLIHGKFEGKLFAV
                     NPKPVSFKEVTYVSNLNNLEAVDLAIIAIPASQCLETVRALLKKGTKAFIVFSAGFSE
                     AGDSGIKLEKELTDIVNKAGGTLIGPNCVGIICETYKGVFTTPVPQYDPQGCELISSS
                     GATAVFIMESALLTGLRFSNVYSIGNAAQTGVEEILEYLDVNFDEHKSPKVQLLYLEQ
                     VKKPFKFLKHATSLIHKGCRIAAIKSGYSEAGGRAASSHTGALATADNVIRALFRKAG
                     IVYCSSREELIAVGSVLQSKELKGENIAIITHAGGSAVMLTDALTSNGLSVPQFDAEK
                     TAVLLSKLNPGSSVNNPIDFLATGTAKHLGEIIDFCEGYDVVDAMVVVFGSPGLFNVK
                     DVYELLDKKMSTCSKPIYPVLPSLINAEKEIKQFLSKRRVNFPDEVNLGKALAHVYTS
                     TKYRFEKTTLPEMDILSIRSIINTAQNGYLDAQANQELLAAAGIELAEQFIVSDKYQF
                     DKVNSKIKFPVAMKVIGPLHKTEVNGVMLNINNIDQAVNAFERLMTIPDASAVLLQEM
                     VKGEELYCGAVRQGDFGHVVLCGLGGVFLELIQDTASALAPMSYGEVKSMIESLKAYP
                     LIKGYRNKKGLNEEKFIEVVMRIGALVHIAPEISELDLNPIKADENNMIVVDSRICID
                     KNGK"
     gene            complement(6110..6802)
                     /locus_tag="PKY09_04385"
     CDS             complement(6110..6802)
                     /locus_tag="PKY09_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMUG2 DNA glycosylase family protein"
                     /protein_id="HNB12126.1"
                     /translation="MRTTFADRVIAFNKNLNYSGKLPEGFDILNPFSDNPETAIVMQQ
                     FYKMYYNDNKKRKFIIGINPSRHGAGVTGVPFTDTKRLESVCKIKMQSAHTHEVSSVF
                     MYDMIQSYGGVKLFYSDFYINSPFPLAIVRRKNNTWLNANYYDDATLFLTLKDYMIET
                     LKNHISLGLITSEVFILGKKNALFISEINKVTKLFKKMTVLDHPRFIQQYRSKEKLIY
                     INKYLSAFRNAE"
     gene            complement(6840..7262)
                     /locus_tag="PKY09_04390"
     CDS             complement(6840..7262)
                     /locus_tag="PKY09_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB12127.1"
                     /translation="MRQKLNLITLGVKNFKKSLDFYEKGLGWKKSDKSMDDLALFQLG
                     GIVLALHPLKDLAADATLNYHASEFYGLTISHNTHSEQEVDEIMKQVASTGGTIVKPA
                     QKVYWGGYSGYFKDLDGYLFEVAYNPFWDMDANDNLLL"
     gene            7420..7758
                     /locus_tag="PKY09_04395"
     CDS             7420..7758
                     /locus_tag="PKY09_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12128.1"
                     /translation="MRKFRILYSILPLICFYSFSFAGGQAEIKPHMSTTQLKSENHKS
                     DVHCDIHHVKVSFIVAEDGLVKVSSIGTRNSMLREYILKKLARLNFKGMNVEGNYSVT
                     LSFDLGDKCT"
     gene            7846..8985
                     /locus_tag="PKY09_04400"
     CDS             7846..8985
                     /locus_tag="PKY09_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNB12129.1"
                     /translation="MDAIYLDYNATTPVDDRVFEKMLPYFSQKFGNAASNTHAFGWAA
                     KEAVEIAREKAAALIRCEPRELYFTSGATEGINLIIKGVYESYSKKGKHIVTYATEHK
                     AVLDTCKYLQTIGADVEVLPVERDGLPDLDLLKKSLRSDTILVMAMFANNETGVLFPI
                     AAMGEICRNHDVIFFSDTTQAAGKVLFDVKECKIDCCVISAHKMYGPKGVGAVYISRK
                     NPRVSVQVQMHGGGHEGGIRSGTLNVPGIVGLGEACHIAEKEMWDNGILLSSLRTRLE
                     QQLETVESVMINGSIKNRLNNTTNLTFKTIRSEDLIKEIRPIAIAAGSACTAALPQPS
                     HVLLAMGLTEKEAYNSVRFSLGKYTTAQEVELTIEKIRKIISSKI"
     gene            8982..11771
                     /gene="polA"
                     /locus_tag="PKY09_04405"
     CDS             8982..11771
                     /gene="polA"
                     /locus_tag="PKY09_04405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNB12130.1"
                     /translation="MNEDKRLFLLDAYALIYRAYFAFSKTPLVNSKGQNVSAVSGFTS
                     TLFDLMRREKPTHIAVVFDAAAETTRAAEHSFYKANRQEMPEDIQTAVPYIKEIIKAF
                     HIPILEYDGYEADDIIGTVAKQKEEEGYTVFMVTPDKDYAQLVSPNVFIYKPGRLGND
                     IEILGVKEIQEKWEVENPKQVIDILGMWGDAVDNIPGIPGVGEKTAKKLIKEYGSMEN
                     VIANAENIKGKLGENIRNNVEQARISKQLATIILDVPVEVNDDDLLMSLPDKEKLSQL
                     FTELEFRSLGKRILGEDFSVNRDLKSATSTSVQTSLFEDESIGSESVVVKGKNIQNTA
                     HTYTLVEHDEAIDDLVKELLNQNEICFDTETSSLDYFSLNIVGLSFSFNSGTGYYIPC
                     PEDFEKAKSICHKFKPVLEHASILKIGQNIKFDMLVLKRYGVEVKAPLYDTMIAHYLV
                     EPDMKHGMDYLSETYLGYTPVHIEELIGKKGKNQGSMRDVPIDKIKEYAAEDADITFQ
                     LKQVLSPMVEKHEVEGVLKDIELPLIPVLADMEYEGVKIDVDYLNVYSKELEQDMLKY
                     RSAIFDIAGLEFNLDSPRQLGDVLFGHLKLPVDKKTATGQHSTNEEVLSKLAQDYPIA
                     EKILEYRELTKLKSTYVDALPQMVNVKTGRVHTTFNQTIASTGRLSSIGPNLQNIPIR
                     TERGQRVRKAFIARDNDHILLSADYSQIELRLAAEISGDENMLEAFRQGLDIHQATAA
                     RVYNVALGDVTKEMRSKAKMVNFGIIYAISAFGLSQRLGIPRKEAAELIENYFVQYPK
                     LRNYMSETLEFARHNGYVKTLMGRRRYLKDINSRNFTVRGFAEREAINSPLQGSAADL
                     VKLAMIHIHEEFKRQNLKSKMTLQVHDELVFDAHKDEVDLIKPIIREKMIHAIKTRVP
                     LEVEIGIGTNWLDAH"
     gene            11828..17035
                     /locus_tag="PKY09_04410"
     CDS             11828..17035
                     /locus_tag="PKY09_04410"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNB12131.1"
                     /translation="MKILNYLCTAFILLLGLSTQAQLTVTSGNPAAAIVDTLVGTGVS
                     VANITYTGNASSKGTFRCTGGCNLGITRGVFLSTGNAGQTPTSPPSDFHSSDMSASGD
                     AQLNLIVAPNLTQDAAVLEFDFTPAADSIGFKFVFASEEYNDYVNTAYNDVFAFYISG
                     PGIPGSPKNLALIPGTSIPVSINNVNNGYSAGVSSGPCNHCTYFVDNVPNGGTANFFD
                     GFTTVISAGIKVMPCQTYHMKFAIADVNDGVFDSGVFLEAKSFSSIGNVGITANGVPI
                     TQNLDTVYACDGDSVVLTASPAGSYNWNNGQTTQTIVVTQANITPSGVYQYAVLNSSP
                     ACFASSKQVKVIFVTPTATITPLGSTALCPGGSVVLQANPGNSYLWSNGATTDTIVVS
                     SAGTYTVTVTQFGNCSAVSTPVVVTQSAPVAIINGVLTICSGSNTTLTATAGTSYQWS
                     TGATTQSVSVGTSNVYTVTVTYSGGCTATASVNVTANTNPVPSITGNNSICQGQSSTI
                     SAGSYSGYSWSTGATTSSININTAGSYSVTVTDANGCTGTATFNLAVNANPTPTITGV
                     TSFCAGGNSTLNAGGGYTNYLWSGGATTQTINVTTGGNYAVTVTNAAGCTATTNKVIT
                     VNALPVPSVTGTLAFCAGKNTTLTVPGSYASYQWSNGATTSNITVNTASTFTVTVTDA
                     NGCTGSKSATTTINANPVPSITGNNSICQGQSSTINAGSYSGYSWSTGATTSSININT
                     AGNYSVTVTDANGCTGTATFNLAVNANPTPTITGVTSFCAGGNSTLNAGGGYTNYLWS
                     GGATTQTINVTTGGNYAVTVTNAAGCTATTNKVITVNALPVPSVTGTLAFCAGKNTTL
                     TVPGSYASYQWSNGATTSNITVNTASTFTVTVTDANGCTGSKSATTTINTNPVPSITG
                     NNSICQGQSSTISAGSYSGYSWSTGATTSSININTAGNYSVTVTDANGCTGTATFNLA
                     VNANPTPAITGITSFCAGGNSTLNAGSGYTNYLWSGGATTQTINVTTSGNYIVTVTNA
                     AGCTATTNKVITVNALPVPTISGPAGICPGASANLVAGSYASYLWSNGNTTSSITTNV
                     SGNYTVTVTDVNGCVGSSNYNLLSFQNPIPVINGMAAICQNQNSILDAGSFASWQWST
                     GAVSQTISVNQSGTYSVTVTNNDGCVASTSFNVVVNPLPVPVISGTSEFCSGNSSVLN
                     IGGGSFASYLWNTSATTSTISVSSGGNYVVTVTDANGCTASTNQVVTVWNLPTPIISG
                     NNAICNGTQTTLTAGSYSNYQWSTGASTDSITVSNGANYIVTVTDVNGCTGTSAPFTV
                     TINQLPTAIISGTTSICIGQSTTLQVQFTGTAPYSYRYSDGTNVSAILNSNITAAQII
                     VTPVSTTDYTLTQISDANCSGNFSGVARVVVNPLPQPVISGSDGECDGISATLTTGNF
                     ASYLWSTGATTQSIAVNSSGSYVCTVTSTAGCKASASHNFETYPLPVVSFTNDSSLTC
                     ELPIINFTNTSQYQLGSVFEWNIDNTVYNSLNATHQFSGSGSYPVSLIITSPNNCVGE
                     SIANVNITVFPLPQASFTIAPDQAPLFNSEVTFGDVSANAVSWLWDFGDGDTSTLQNV
                     SHFYADPGKYNAKLYVTNVAGCIDSLAREIFITPFYIPNAFTPNGDGRNETFFDPGYA
                     TNVQSLTMNIYNRWGQLVYTTDNLSKTWDGVDSKGNLSPQGVYVYALKVTTYSGKHYD
                     YTGSVSLIR"
     gene            complement(17042..17749)
                     /locus_tag="PKY09_04415"
     CDS             complement(17042..17749)
                     /locus_tag="PKY09_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNB12132.1"
                     /translation="MKRFLLIALVFIGQQTYAQKKIFKATNNTPSNQGEGLANNDIIS
                     GLREALKVGTNNSTASASKLDGFYKNPLIKIPFPREIRDAENTLRSMGMDKEVDKFVK
                     TLNRAAEDAAKSAAPIFINSIQHITINDGLAILRGGNDAATQFLKTTSNAALIAAFKP
                     IVQTSLNKVKITQYWNPLMSTYNKVPFVKKVNPDLNQYVTDKAIEGLFKLIAAEELKI
                     RKDPAARINDILKSVFG"
     gene            17880..19124
                     /locus_tag="PKY09_04420"
     CDS             17880..19124
                     /locus_tag="PKY09_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNB12133.1"
                     /translation="MITNVELINIGDELLIGQIVNTNATWIGQQLSLSGYWLKQITTI
                     GDNEQEIIDALNAAKSRAQIILITGGLGPTKDDLTKDVLCKYFNTHLVFDKQAYSDIE
                     NIFHARGKVVTETNKRQAELPSACVPIYNKKGTAPGMWFNYGEHVFVSMPGVPFEMQA
                     MMQNDVLPKLKQQYKAMPIVHKTILTQGIGESDLSDRIASWENDLPPHFKLAYLPAAG
                     MVRLRLTGKGVEDMERDMNQRISKLKELIAPYIFGYDDDTIQSVVGSLLLEKQKTIAV
                     AESCTGGFLSHLITSIPGSSAYFMGGTVSYSNASKMSQLSVSEQSLKDYGAVSEAVAI
                     EMAIGARKIYNTDFALSITGIAGPGGGSVEKPIGTVWMGIATHDSCYARKFLLGTNRL
                     RIIQVAADTALNMLRKEIIEHY"
     gene            19181..19252
                     /locus_tag="PKY09_04425"
     tRNA            19181..19252
                     /locus_tag="PKY09_04425"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19213..19215,aa:Met,seq:cat)
     gene            19439..19864
                     /locus_tag="PKY09_04430"
     CDS             19439..19864
                     /locus_tag="PKY09_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB12134.1"
                     /translation="MTHLINIKSRMAIKEYTLLLFRIGVSLLMLYGHGYPKLQKIFSG
                     AEIQFADPFGLGMKMSFYLVVFAEVICSIFIILGLKTRMASSVLVFNMLVATFVMASK
                     GEVPELALMYFFCYVLILIFGAGKYSVDYNLNSKKKGKF"
     gene            complement(19936..20760)
                     /locus_tag="PKY09_04435"
     CDS             complement(19936..20760)
                     /locus_tag="PKY09_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNB12135.1"
                     /translation="MNNFWTRAFTGAVFVAVMIAAIEFSPYTFMILFMVINLLSIIEF
                     YKLFIRNDLTPRIFSGIVLSAILYATSALVASGTASAQLLFINIPMLFLIFIFELYLK
                     SEKPFENIAYTLLGVAYLTLPLALFNSMAFLPGENTGYHSGIIIGYFLILWASDTGAY
                     LSGMKFGKHKLFERHSPKKTWEGSIGGCLAALLLGYINSLFFTQLALSEWLIVSLLIV
                     ITGTFGDLIESMLKRSLKIKDSGNILPGHGGMLDRFDGLFISIPFVFTYLALIGKV"
     gene            complement(20757..20972)
                     /locus_tag="PKY09_04440"
     CDS             complement(20757..20972)
                     /locus_tag="PKY09_04440"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNB12136.1"
                     /translation="MDKPAEWVKVYTTTQPHKVEIVRAVLEEHDIESMVINKNDSSYP
                     GLIGNIEIYVLDKLEVLARFIIKDNEL"
     gene            complement(20979..23597)
                     /locus_tag="PKY09_04445"
     CDS             complement(20979..23597)
                     /locus_tag="PKY09_04445"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNB12137.1"
                     /translation="MSELSKIYNPQETESKWYAHWMNEKFFKSVPDERPPYTIVIPPP
                     NVTGVLHMGHMLNNTIQDVLIRRARMLGKNACWVPGTDHASIATEAKVVQMLRDRGIK
                     KSDLTREEFLKYAWEWKEKYGGIILEQLKKLGCSCDWDRTRFTMEDDLSEAVIDVFID
                     LYNKGYIYRGLRMVNWDPAGLTAVSDEEVIHKDVNSKLYYVRYKIDGTADEWITIATV
                     RPETILGDTAVCVHPEDERYTHLKGKFCIIPIVNRRVPIIFDDYIDKEFGTGALKVTP
                     AHDINDYNLGLKHKLEVIDTLTPDGKMSEAAGHYIGEDRFIVRKKIAKDLEASGHLIR
                     TEDYKNKVGYSERTDAVIEPRLSLQWFVNMPEISKPALEAVVNGNIKLIPDKFISTYR
                     YWMENVKDWCISRQLWWGQRIPAWYDESGNFVVAKTPQEALAKFEEKNIKINADKIKQ
                     DDDVLDTWFSSWLWPISVFDGFKDPTNKEIKYYYPTNDLVTAPEILFFWVARMIIAGL
                     EYRNDIPFERVYLTGIVRDKLGRKMSKSLGNSPDPLELIDKYGADGVRVGMLLSSPAG
                     NDLLFDESYCEQGRNFANKMWNAFRLIKNWQPDASLRQSETGRIATAWMKSKFNEQLT
                     LINDSYDKMRISEVLMIKYKLFWDDFCAWYLEMIKPPYGSVAIDSETYNTTIDIFENL
                     LQILHPFMPFITEELWHELKNRNYRDCIIVSMWPKQASTDNAILEGMTHAQNIISTLR
                     TFRNEKGISPKTPLQLLTKEKHLSTFDNIILRLANVSSIEKANSFDKAFAFMAGTQEY
                     AIPFDQNIDTAAERLRIEKEIAYNKGFLDTVMKKLGNANFVSKAKPEVIASEEKKKSD
                     AEQKIKSLEQQLNMMS"
     gene            complement(23718..26141)
                     /locus_tag="PKY09_04450"
     CDS             complement(23718..26141)
                     /locus_tag="PKY09_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12138.1"
                     /translation="MKSILNILAGITLLTQPVMSQTIQAKITDNNNEPLTGAIAELRQ
                     IKDSTLSKVAVADVSGKVIFEQIKPGSYFLKTTFLGFNNYLSPAFDFKEGDNKDFHTI
                     KLSSSSTNLKQATITAFKPLVEVKADKTVFNVENSINSTGSTALELLQKAPGVVVDNN
                     DNIILKGRGGVMVQIDGREIRMTESELGDYLRSIQSTDVESIEIISNPSSKYDAQGTA
                     GIINIKLKKNKNYGTNGSITAGYAVGTYSKYNTSLSVNNRTKKFNIYSTYSNNWGDRK
                     NEFYLYREQNPFTFNQSSKSKRGGLSHNYKVGVDYQMNANNSFGIMINGNYSDITGRQ
                     KNKNFISNFYSGVTDSILNSNQRYNSINNNFYANLNHHYKDTTGKELTTDFDYGYYNG
                     TRNTYLPNEYTLPDGTTPLSATYYKSNTPTTINIYTLKTDYTQNLFKGKLGAGYKVSL
                     VNTVNTFNFYNIEGGIASLDSSRSNKFDYAENVNALYLNYQRTFNKIDVQIGVRMENT
                     NSNGKLKTSATANDKDVKRSYTDFFPSAGITYNINKDNSAGLIYSSRIDRPNYQELNP
                     FEYKLDELSFRKGNPFLDPQYSNKIELTHTYKYIITTSAGYSHTKDFFAQITDTLGEG
                     KSYLMPRNLATEDVLSVSLSASLQPLKWYSIYLNASVDNQKYKADYGGNKTINAQFTS
                     FNLYGQNTFKLPYQFTFELSGWYNSGGVWGGSFKTKAQGSLDVGLQKKLLKDQATLKI
                     SYTDLLHTTPWDSYNTYAGIVSRAWGNWESQQLRLSFTWRFGNKQMKNIRQRSSGSEQ
                     EQKRIGGGE"
     gene            complement(26194..28680)
                     /locus_tag="PKY09_04455"
     CDS             complement(26194..28680)
                     /locus_tag="PKY09_04455"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNB12139.1"
                     /translation="MLHKVIHVLFLSVLFSGVVSASDSLKHYKATRIFEPIKIDGQLT
                     ESVWASTNALSDFVMNRPIEGGVPTQKTEVRIVYDNTAIYIGAMLYDSAPDSILKELG
                     LRDGTDPSSNSGFTDINADYFRFVFDPYNTRQDAYDFGVYASGIQADSRYSDYLFDAV
                     WESSVKITDKGWCVEIKIPYSAIRFPSKPIQEWAFQITRNIRRNREFQQWSLTPSTAA
                     NAQNYWGVLDGIENIKPPLRLSLTPYISVAYEHEPVSGSKANQTYSYRGGADIKYGLD
                     ERFTLDMTLLPDFGQVQSDNKRKTLSYEEINYDENRQFFKEGTDIFSRDNLFYSRRIG
                     QTPSRYYDLMNKYDESDIKSNPTESKLINATKLSGRTNEGIGLGFFNAITNNTYAIIK
                     DKNTGLTEKILTEPLANYNVIVADKQWKSNSSVYLINTNVTRTKKFSDSNVTGGGFKL
                     VNKKNTYSLSGGLDYSIKFITDPDGINSDKKTDGLRHYLAIKKTSGTIRWGLSQVGVN
                     PTYDPTDFGYYVTPNRINSNANITFFQFKPYGPVQEGNWRFDLNYSQHYKTGYMRETS
                     LQTNLFILFKSYTAIFGGGGFTPAAGREYDPRLNGRYVNALKYWFAFIGVSTDYRKAL
                     ALDMEINISNFIKTYLSEGGNFDATLRYRINDKLTLNASSEYNFDPYNFGFTSETDPN
                     IYLFGLRRTNTYINSLAARYIFKNDLSLSIVARHYWFNYAYRKFFFLQDDGGLSPTTD
                     IYNNEFDGSYNFFNADLLFSWRFAPGSTLTFSYKNIFDNGTDGKLETASFTKNLDYVF
                     KNPHVQTFSIKVLYYLDYLQLRKNQTSTEN"
     gene            28838..30055
                     /locus_tag="PKY09_04460"
     CDS             28838..30055
                     /locus_tag="PKY09_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNB12140.1"
                     /translation="MKVSLTARIFIGMLLAILLGHWYNISHTAAELDVFASRISILSD
                     IFLRLIKMIIAPLVFATLVVGVAKVGDFKSVGRIGLKTILYFQFATILALLLGLLLVN
                     FFEPGKIMNLQIPAQSASSGVSAKGLNAKDFITHVIPKSIVEAMANNDILPIVVFALF
                     FGLAAASVGEKAKMVVNAMDAVSHIMFKVTNFVMKFAPYGVFGAVAAVVAKQGLSVIS
                     GYLYLIATFYGGLLFFGLVILGLICYFLKIPFVKLLQHIREPILLAFSTASSESAFPK
                     TIEALEKYGCSNRIISFVLPLGYSFNLDGSIMYMAFATQFIAQAYGMDLNIQQQIMML
                     LMLLVTSKGMAGVPRASMVVIAGMLESFNIPEAGLLLILGVDQILDMGRSATNVVGNA
                     VATAVVSKWEKEL"
     gene            30158..30664
                     /locus_tag="PKY09_04465"
     CDS             30158..30664
                     /locus_tag="PKY09_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNB12141.1"
                     /translation="MKKLFLAVVIFCSATMVKAQDDSKAAPKPTATPAISPNAADISF
                     EKETHDFGTIPYNGNGTYEFKFTNTGKEPLVISNAKGSCGCTVPSWPKEPILKGHSGV
                     INVHYDTKRPGPFTKTVTVSSNGKTAEKVLTIKGNVETKEQTESGTPVKKDNTMAPKE
                     TTTNPFGN"
     gene            30718..31164
                     /locus_tag="PKY09_04470"
     CDS             30718..31164
                     /locus_tag="PKY09_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNB12142.1"
                     /translation="MKKTILLSALAIGMATATFAQDKAAAVQVAPAENKNQADFKFET
                     LEYNYGTIKQGDKVNYTFNFVNTGKEPLIITNAQGSCGCTVPEWPKEPIKKGEAGKIH
                     VTFNSAGKMGLQDKTITITSNAKTSPVVLHIKGNIEAAATQEAPKN"
     gene            31258..32487
                     /locus_tag="PKY09_04475"
     CDS             31258..32487
                     /locus_tag="PKY09_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB12143.1"
                     /translation="MPSISEKGKHMPPSPIRKLVPYADKAKAEGKKIYHLNIGQPDIE
                     SPKVMLDAIRNIDLKVIEYSHSAGIESYRRKLISYYKKYNININFEDILITTGGSEAI
                     EIAMMTCLNEGDEIIIPEPFYANYNGFSTQAGVTVVPVFSSIRNGFALPPHDVFEKVI
                     TPRTKAIMICNPSNPTGHLYSKEELQSLRDLALKHDLYLFADEVYREFCYDNNSYFSI
                     MHLEGLDENIILLDSISKRYSACGARIGAMISKNKKVMSTAMKFAQARLSPPTFGQIA
                     AEAAIDTPQEYFDNVKAEYVARRNFVIEQLNSMPGVFCAKPSGAFYCMAELPIDDSDT
                     FCQWLLEKFSYKGQTVMLAPATGFYSHAELGKKEVRIAYVLNLDDLKNAMICLNEALK
                     VYPGRVENISNNTIVMQ"
     gene            32531..33451
                     /locus_tag="PKY09_04480"
     CDS             32531..33451
                     /locus_tag="PKY09_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNB12144.1"
                     /translation="MIPDYQSLMLPLMKLVSDGQEHKYRDLIEKLAVEFQITNEERKE
                     LLTSGNQAIFDNRVGWAKTYLKKAGLLDSPKRATFIITDLGRETLSKNPDRIDAKYLK
                     QFPAFMEFIKASRNNNESEEDETSIIENNEQTPEESLDKAYQRIRKSLASELLNKVVD
                     LSPAFFERLVVELLVKMGYGGSIKDAGKAMGKSGDEGIDGTIKEDKLGLDIIYIQAKR
                     WKPGNVIGRPELQKFVGALAGQGAKKGIFITTSNFTKEALDYTPRNETKIVLIDGEQL
                     AQLMIDYNLGCTPQQTYEIKKIDSDYFGEE"
     gene            complement(33688..34278)
                     /locus_tag="PKY09_04485"
     CDS             complement(33688..34278)
                     /locus_tag="PKY09_04485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNB12145.1"
                     /translation="MIKIFAGNKTFYFTNNADSFSDDVIKFENSIDKDKLKQILSDSK
                     SMEIVCLCDNAESGVRDFIKLFTYIAAAGGVVYNHEKKLLLIHRLGKWDLPKGKMENG
                     ESPETSAMREVCEETGVCNLKIIKPLTDTYHIYHQNGEYILKRTYWFEMTTSSTTNLL
                     PQTEESITEVKWMTKAEANAAMQFSYPSIRSLLSQD"
     gene            34323..34958
                     /gene="pyrE"
                     /locus_tag="PKY09_04490"
     CDS             34323..34958
                     /gene="pyrE"
                     /locus_tag="PKY09_04490"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNB12146.1"
                     /translation="MKDDTASNIADFLLQIKAIKLQPDEPFTWASGWKSPIYCDNRVA
                     LSNPRIRTYIRQELVKAIERNYGRPDAIAAVATGAIAQGALVSESMGLPFIYVRPEPK
                     QHGAKNQIEGDISQIHSVVVVEDLISTGKSSLNAVAALRNSGIIVKGMVSIFNYGFDI
                     ARESFKNAQCPLYSLSNYDVMITKALQSKYINEGQMESLKDWREHPDSWNK"
     gene            34966..35367
                     /locus_tag="PKY09_04495"
     CDS             34966..35367
                     /locus_tag="PKY09_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNB12147.1"
                     /translation="MTRIESDECVIDKPANEVFAFLSNCNNLQQLMPEQVTNWKSTND
                     DCSFTIAGMASLGMEIKERNPDQNIRVERKGKAPFDFVMNYEMQAVEDNKTKLKIAFD
                     ADLNPMLKMMAEKPLRNFLNTLAGNYKNLNQ"
     gene            complement(35379..35849)
                     /locus_tag="PKY09_04500"
     CDS             complement(35379..35849)
                     /locus_tag="PKY09_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12148.1"
                     /translation="MVKQLCCNFGRLTSKVMRLKNIILISTLIFLFSGCRKKENGIVP
                     YAYVNVIFYANDPQLVQLNAVGNYIYYPAGYKGIIIYRRSINEFVAYERACTYDAESS
                     CDGVTVMSDNFTLRDSCCGSKFMIYDGSISQGPATHALVQYATFYDGTILRITN"
     gene            35916..36134
                     /locus_tag="PKY09_04505"
     CDS             35916..36134
                     /locus_tag="PKY09_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12149.1"
                     /translation="MISTTVVFAQSKASKAQRKAEKKKEMEKKKALKAEDKARKHHMK
                     IQTKEVRKRMKKNRKKSARETFRRSESY"
     gene            complement(36142..>38051)
                     /locus_tag="PKY09_04510"
     CDS             complement(36142..>38051)
                     /locus_tag="PKY09_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNB12150.1"
                     /translation="SSEQAWNGLNKLNSEGKGRAHFFILDSFTSYQQTSTHQIEGCIA
                     ASTVIENDGRFQSLMNYLLANVYIAQNNDVATHANQNEIDGKIILSPDGKFYRQRYAI
                     SGGSVGSYDGKRTGRLKHLEKLLAEIQQHQQQTEVLKIELQQTEELLSAGKTQATENR
                     KIISLTDSELSKAVLNINTLLSKKDFITSNCESNEKNLQKLQEELQLTQEQKNNAPGF
                     SSMSLDEMKINLQKLTDQQREKQNQSGQLQKISQDASNAFNQKNITFLQQQNKVQNII
                     REIGYKNNQVVSLRSSVEKIKTEIEQLHEQINDTAQAGNSSETDLKTQVEEKVLLESR
                     LGESEKNYFEAKGKIDQFDKQIAETRRKREMADELMHALHNEVSDVNLKMTSLKERLS
                     VEFSLEVEDIVSQEANPEWNEEDLKVRNEKLGGQLKNFGPINPMALDSFKEIKERYDF
                     IIKEQDDLKKAKDALLQTIAEIDNSAKEKFSEVFNQVRNNFINVFRSLFTADDNCDMI
                     LADTGNPLESDIEIIAQPKGKKPLSIQQLSGGERTLTATALLFGLYLVKPAPFCIFDE
                     VDAPLDDNNIDKFNNIIKKFSKDSQFIIITHNKKTMAATDIIYGITMNEPGVTAVVPV
                     DLREYADTIEA"
BASE COUNT        11780 a         6709 c         7265 g        12297 t
ORIGIN      
        1 cacgtatatg gtttgagaca aacggttacg gagcagtttt tacaggttca aattatcagg
       61 gaggaaatga ccttaccccg caatcaggaa tgaatgagta tgggctttcg tttggcactt
      121 tagcaacccc cacacccgac aaggtaaaat cattcgtgaa caagaaacca attcaaagca
      181 ggtcaaaata cctaaaagac attttacatt cctgtaaaaa cgttgaagaa gtacaagcat
      241 ttattgaaga atatgaccat agtacatttt ccaatgatgt atttatctat acagacaaat
      301 cagggaagta tctaattgtt gagccctatg aactaattcc tggcaacgac aacaagtatg
      361 tacttgctaa cttttgccct tcaacaatta ctgactttgg cagtattaag caacagcgtt
      421 atgttgacgg agttgcattt ttgaaagaca agattgacac ttcacttgca ttttgcactg
      481 cactgtcaga tacaatgcat gtgtgcagaa aaaaaattgg tgacggtact ttgctaacct
      541 caattttgga cttgaacaat ggaatagttc atttatactt ttaccatgac tataaacaca
      601 tggttcaatt caacctaaaa gaggagttgt taaaaggaaa tcactcctat gaaatagcct
      661 cattatttcc agcgaacatt gaatatcaaa aacttcttga ctttcagaca ccattaaata
      721 acaatacaat tgactggttt cttcgcctct gcctgatact ttttgtattt tcatttttct
      781 actttctaat cagttatatt aaaaacagaa aaacagagct tgcaaactat aaattgctgc
      841 tggcgtcatt gagtttgtgc ttggcctatt acatgtttgc tttagccaca gtaatggcaa
      901 ttttctattt cccttctcca tataaaagct acaaattcgg attgattgat attgcttctt
      961 acctaccgta cttactaata ttacttataa ttccattatt gataattaac cgaaaggttt
     1021 ttaaggtgaa tacttggaag acaacttcaa agtttctttt tactgctaac aacattgcat
     1081 acttaatttt aatttcgctg ttttgctatt ggggtttcta caatatttaa tgcaactatg
     1141 acaggattaa acaagtgcaa cagagaggca aataagcaag ccccctaata aaatattgtc
     1201 aattgttggg atgaaaacct tgattgaaat tttgggctgc aatattttgt tagaaaaaat
     1261 tgagatgtaa tgctatcgaa ggattacaat atacaaaact gtgcgaatta agatttgttt
     1321 tttgagagac ctgagtttat ttaataaaaa agctgccttt aactggcagc tttttggcaa
     1381 ttgttttatg cctttattat ttaatcaacc attaaactta gtagcttctg actatctgat
     1441 gtgtctgacc atttgcactt gtccagttga gtttgtatcc gtaagggcaa ccacttgttt
     1501 gtgcatttcc ttgttgatct acaccaaaag ttactgtcaa ctctcgtacc acaccatgat
     1561 gcactcttac tccactcata ggattataat tacaatctga actgagaata ataggcgtat
     1621 cagtagatac aaagaaggtg tttcctgcac ggtttacacc ccaaacagat acatttgaat
     1681 agccaccttg tgatgcatta cctgtgatgg ttattgtagt aacattattt gttctttcaa
     1741 caagtctgtt acgatcaaaa ttccatgatc ttgttgtgcc gtcatcaaat gttaccatgg
     1801 tattaccggt tgcatgatgc agaatcggag tggtaaagtt tggtgagttg tcaatcaaac
     1861 cgccatttac gtttgtaatg gttcgtgttg agtttatggt aattgatttg ccactgctca
     1921 cattggtaat ttcaacattg tttagtgtta atgtcaactg gctaccagca gtactccatg
     1981 gtgtaacggt gcttgtattt gcatcataag gaagctggat aatcattgtt cctgtgcggt
     2041 tgcgtgtgcc attgcaattg agtccgttaa aagtaactgt aacttttccc tgactgatta
     2101 aagaagtatc aattgtagtg ttacaaggta cgacaaggcc attgtaaatt cccataaaat
     2161 taatgcctct taggcgtgga tgcatagcaa taatacgatt agcatcatca agttcttcat
     2221 tgtctgatgc tgcaattctg gcatcatcat ctgcagttac ttgctgtgta gtttgttcgt
     2281 ctaataaatc ttctgattta tcttttttac atgctgccat taaactgatc ccggcagcta
     2341 agcttaacat taaaataccc tttttcattt ttctgaattt tattgatttg tacttatgac
     2401 ttcagaaaat attattggtt taatctgcct gaaatatttt ttttaattga ttgtgtttcg
     2461 ttatttatta ttggttgact ttttcaatca ggtattttta atgaatatac cgaaagtaat
     2521 gtgtcatttt ctaactggtc gtattcatat tttagaccta atttagtcaa taattttatt
     2581 gatttaaaat tgtcaggctt tgtaacgcca attattttag aataagattt ggccttaatg
     2641 agtaagttta aataaaagct acttgcttca aaagcatatc cataaccggt atattgaggt
     2701 aaaaaagcaa aaccgatgtc aggaaattgc tgcttgtccc gattcaataa agttataatg
     2761 ccaacaggct ttttgtcaga ttttagttct attatatgat agtagtaaac tgagtgtgcc
     2821 aatatgtttt caatatattt taatgcagca tcatgattat ggatatttct ttcaccaata
     2881 aattctttcc attcgtctgt gtttaacaac tccagaatga ataaagtatc atttgtattt
     2941 aatggccgta aatgtagtcg ttgtgtttct ttgtctataa acatgaaaat attattgcct
     3001 gaaattgttt tatgtaaaaa ttgaacagca attacaaaaa tataaaacaa tttcaggcaa
     3061 cttattcctg atgaataaca aaagactaaa aatattattc tttaactttt ctgactctta
     3121 ccttccattc ttcggggcct tttaaaacgt attcccattt gaagggtact tcgctttctg
     3181 cttccatctg atagtagagt ggaagaggat catgatcatt aataaattca aatgcttcac
     3241 cgggatttat agcagcaaag gcatcataaa acatttcatg tcttgttgcc ggaggaaatg
     3301 gtctcaagtc aaattcttca actacggaaa atccatcatc acctaagcca ctgctttcag
     3361 tttttgtgat atgcaccacc acttcaccat cttcttgctt cttataaatc caacggtgct
     3421 taccttcaaa cttttgaata aagatgccat gtatttctgt tggatcttcg gcagcaataa
     3481 tctccattgt actgccttgc tccatcatac cataacgatg aaacacgatt tgtttccggt
     3541 cagcaacagc agcttttctt aaatccaata atgtagagat gtcagaaaat tctctgcctt
     3601 gcgaagcttc cgtacgtgta actttcactt tccattctct accgcctttg tttaaataat
     3661 cccaacctac tacatcgcca tagattgaac gaaactcata ataaagcggt atgggatcat
     3721 gatcgttaat aaaaataaaa ctttcaccta cgggaagttc tttaaataac tgaatcagtt
     3781 ttttatgtct ttcaatcgga acaaacacgc gcatgtccat ttcttctgtt gttttgttga
     3841 ttatcataat aaaagtattt aaatacttgc aaaagtaaaa tttaattctg ttattgaatg
     3901 taaaagtcct gtcacaattc ggtttatttg ttttgatgaa tcaatttcac tgcacttcag
     3961 tcagaaaagc tttaaaatat gattcgggtc atattacaac aggtttaatg aatgtagttt
     4021 tgcaactttt gaaagagaaa ttagataatg gttatttcgc agcttattca gcccaaaagc
     4081 attgctgtaa ttggtgcttc tgacaatatt accaaacccg gtggtatggt attgcagaat
     4141 cttattcatg gaaaatttga aggaaaatta tttgcagtaa atcccaagcc ggtaagtttt
     4201 aaagaagtta cttatgtttc caatctaaat aatttggaag ctgttgattt ggcaattatt
     4261 gctattcctg caagtcagtg tttggaaact gtcagggcat tgctaaagaa aggtactaaa
     4321 gcgtttattg ttttttcagc aggtttttca gaggcaggcg atagcggaat aaaactcgaa
     4381 aaagagctta ctgatatagt gaataaagca ggaggcactt taattggtcc caattgtgtt
     4441 ggcatcattt gcgaaactta caaaggtgtt tttactactc ctgtccctca gtatgatcct
     4501 cagggatgcg aattaatttc aagctcaggt gcaacagccg tttttattat ggagtctgct
     4561 ttactaactg gattaaggtt ttctaatgtt tattcaatag ggaatgcagc gcagacaggt
     4621 gttgaagaaa ttcttgaata tcttgatgta aattttgatg aacataagag tccaaaggtt
     4681 cagttgcttt atcttgagca ggtaaaaaag cctttcaagt ttctgaaaca tgcaacatca
     4741 cttattcaca aaggctgtcg tattgctgcc atcaaatcag gttattcaga agcagggggt
     4801 agagctgcat catctcatac cggtgcatta gccacagcag ataatgtaat ccgtgccttg
     4861 tttcgcaagg caggtattgt atattgcagt agtagagaag agttaatcgc agtaggaagt
     4921 gttttgcagt caaaagaatt gaaaggagaa aatattgcaa taatcactca tgcaggaggt
     4981 tctgcagtca tgcttactga tgcactaact tcaaacggat tgagtgtgcc tcaatttgat
     5041 gccgaaaaga cagcagtatt gctgtcaaaa cttaacccgg gatcgtctgt gaataatcca
     5101 attgattttt tagcaaccgg aacagcaaaa catcttggtg agattattga tttttgtgaa
     5161 ggctatgatg ttgttgatgc aatggtcgtg gttttcggaa gcccgggatt gtttaatgta
     5221 aaagatgtgt atgagttgct tgataaaaaa atgagtacgt gttcaaaacc aatttatccg
     5281 gtactaccct cgttgattaa tgcggagaag gagattaaac aatttttgtc aaagcgaaga
     5341 gttaattttc ctgatgaggt aaatcttgga aaagcattgg ctcatgtata tacatcaaca
     5401 aagtatcgtt ttgagaaaac caccttacct gagatggata ttttgtccat acgctcaatc
     5461 attaataccg ctcaaaatgg ttatcttgat gcacaagcaa atcaggagtt acttgcggca
     5521 gccggtatag aactggctga gcagtttatt gtttcagaca agtatcagtt tgataaagtc
     5581 aatagcaaga taaaatttcc tgttgccatg aaagtgatag ggcctttgca taaaacagaa
     5641 gtcaatggcg tgatgttaaa tattaataac atagatcagg cagtaaatgc attcgaaaga
     5701 ctcatgacaa ttcctgatgc atctgcagtt ttacttcagg aaatggttaa aggtgaggag
     5761 ttatactgtg gtgctgtaag acaaggagat ttcggtcatg tagtattatg tggccttggt
     5821 ggtgtgtttc ttgaattgat acaggatacg gcttctgcac tggcaccaat gtcttatggc
     5881 gaagtaaaat caatgattga atcgctaaaa gcctatccgc ttatcaaagg atatagaaat
     5941 aaaaaagggt tgaatgagga aaagtttatt gaagttgtta tgcgcattgg tgcacttgtg
     6001 catattgcac cggaaatcag cgaactggat ttgaacccga ttaaagctga tgaaaacaat
     6061 atgatagttg ttgattcaag aatttgtatt gacaaaaacg gcaaatagtt tattcagcat
     6121 ttctgaatgc actcaggtat ttattgatgt aaattaattt ttctttcgat ctgtattgtt
     6181 gtataaatct tggatggtct aagactgtca tttttttaaa cagtttagtt accttgttta
     6241 tctcacttat gaataaagcg ttttttttac ctaaaataaa aacctctgaa gtaattaatc
     6301 ccagactgat atgatttttt aatgtttcaa tcatataatc ttttagtgtt aaaaacaatg
     6361 tggcatcatc atagtaattt gcgttgagcc atgtattgtt tttcctgcgc acaatagcta
     6421 aaggaaaagg agagttaatg taaaaatcac tatagaacaa tttaacacca ccgtatgatt
     6481 gaatcatatc gtacataaat acagaagaaa cttcatgagt gtgcgcagat tgcattttta
     6541 ttttgcaaac actttctaaa cgtttggtat ctgtgaaagg tactccggta acacctgcac
     6601 catgccggct tggatttatg ccaataatga atttcctttt tttattgtcg ttataataca
     6661 ttttataaaa ctgttgcatg acaatagcag tttcgggatt gtcgctaaaa ggattaagaa
     6721 tatcaaaacc ttctggtaat ttgccggaat aatttagatt tttattgaat gctattaccc
     6781 tgtctgcaaa ggtggttctc actaattgta atttttttaa attatctttg cttagaagat
     6841 tataaaagaa gattatcatt ggcatccatg tcccaaaaag gattgtaggc aacttcaaaa
     6901 agataaccat ccaaatcttt aaagtaacca ctatagccac cccaataaac tttttgtgcc
     6961 ggttttacta ttgtcccacc agttgaagca acctgcttca ttatttcgtc aacttcttgt
     7021 tcagaatgtg tattgtgtga tattgtaagg ccataaaatt ctgatgcgtg ataattaagt
     7081 gttgcatcag cagcaaggtc tttcagagga tgtagtgcca gaacaatgcc acctagttga
     7141 aacaaggcaa gatcgtccat gcttttatcc gactttttcc agcctagacc tttctcgtaa
     7201 aagtcaagag attttttaaa atttttaact cctaaagtaa tcagatttaa tttctgtcgc
     7261 atcacattaa cttttaaact tattgtgcac ttatgggtca aacttagtga tacttttaaa
     7321 aagttctaaa aaattgtttg ttatattgaa ttgtttttta catttgggta actttttgtt
     7381 cgttatgggc gtcatatatt aaacttaaaa gttacaatca tgagaaagtt ccgcatactt
     7441 tattctatcc tgccactaat ctgtttttat agtttttcat ttgcaggtgg gcaggctgaa
     7501 atcaagccac atatgtcaac cacacagtta aagtctgaga atcataagtc tgatgtgcat
     7561 tgcgatattc atcatgttaa agtttcattt atcgttgctg aagatggttt ggtaaaagtt
     7621 tcaagtattg gtacccgtaa ctccatgttg agagaataca ttttgaagaa acttgccagg
     7681 ttgaatttta aaggaatgaa tgtggaaggc aattacagtg ttacacttag ttttgatctg
     7741 ggagataagt gtacgtaagc aatttagttt tttaggcata ctttatttag tgtagcttat
     7801 ttttatactg agataggcaa aatattttct ttctttgacc tgtagatgga tgctatatat
     7861 ttagactata atgctactac acctgtagat gatcgtgtgt ttgaaaaaat gttgccttat
     7921 ttttctcaaa aatttggcaa tgccgcaagt aatacacatg cttttggatg ggcagcaaaa
     7981 gaagccgttg aaatagctcg tgaaaaagcc gctgcactta taagatgtga gcctcgtgaa
     8041 ctgtacttta cttcaggtgc cacagaagga ataaatctga ttataaaagg tgtttacgaa
     8101 agttattcaa aaaaaggaaa acacatagta acctatgcta ctgaacataa agctgttctc
     8161 gatacctgta agtatttgca gactattggt gctgatgttg aagtcttgcc tgttgaaaga
     8221 gatggcttgc ctgatcttga tttgttgaaa aagtctttac gctcagatac cattttggta
     8281 atggccatgt ttgcaaacaa cgaaacaggt gttttgtttc ccattgcagc aatgggtgag
     8341 atttgcagaa accatgatgt gatttttttt tctgatacaa ctcaggctgc aggtaaagtt
     8401 ttgtttgatg tcaaagaatg taaaatagac tgttgtgtga tttctgcaca taaaatgtac
     8461 ggacccaagg gagtaggagc ggtgtatatc agccgaaaaa atccgagggt ttctgttcag
     8521 gtgcaaatgc atggtggtgg tcatgaaggt ggtattcgct ccggaacatt aaatgttccg
     8581 ggtattgttg gacttggtga agcttgtcat attgcagaga aagaaatgtg ggacaatgga
     8641 attttattat catcacttcg cacaagactt gagcagcaat tggagaccgt tgagagtgtg
     8701 atgataaatg gaagcataaa gaatcgtttg aataatacta ccaatctcac ttttaaaaca
     8761 ataagaagcg aagatctcat taaagaaatc aggcctattg ctattgcagc cggatctgca
     8821 tgtactgcgg cattgccaca accgtcacat gtattgcttg caatggggtt gacagaaaaa
     8881 gaagcttata attctgtgcg attcagtctt ggaaaatata ctaccgcaca agaagttgaa
     8941 ttaacaattg aaaaaatcag gaaaataata tcttcaaaaa tatgaatgaa gacaaacgcc
     9001 ttttcttact cgatgcttat gcattaatat atcgggcata ttttgcattc agcaaaacgc
     9061 cattagttaa cagcaagggg cagaatgtgt ctgccgtaag tggatttact tcaacacttt
     9121 ttgatttgat gcgaagagaa aaacctacac atattgccgt agtgtttgat gccgctgcag
     9181 aaaccacacg tgccgctgaa catagttttt ataaggcaaa ccgacaagag atgcctgaag
     9241 atatacagac agcagttccg tatatcaaag aaattatcaa agcatttcat attccaattt
     9301 tggaatacga tggttatgaa gctgacgata tcataggaac tgtggccaaa caaaaggaag
     9361 aagaaggtta cactgtattt atggtaacac cggataagga ctatgcacag ttggtatcac
     9421 ccaatgtttt tatttataag cccggacgtt taggcaacga tattgagatt ttgggagtaa
     9481 aggaaattca ggaaaagtgg gaagtagaaa acccaaaaca ggtaattgat attttgggta
     9541 tgtggggtga tgctgttgac aacatacctg gcatacccgg tgtgggtgaa aaaactgcaa
     9601 aaaaattaat taaagaatat ggcagcatgg agaatgtgat tgccaatgca gaaaatatta
     9661 aaggtaaact tggtgagaat atccgcaata atgttgagca ggcacgaatt tcaaaacagc
     9721 ttgctacaat aattcttgat gttcctgttg aagttaatga tgatgatttg ttgatgtcgt
     9781 tacctgacaa agaaaaatta tctcaacttt ttacagaact agaattcaga tctctgggca
     9841 agagaattct tggcgaagat ttttctgtca atcgtgactt aaaatcagcc acatccactt
     9901 cagttcagac aagtttattt gaagatgaga gtataggaag tgaaagtgta gttgtcaaag
     9961 gaaagaatat tcaaaataca gcacatacct atactttggt agagcatgat gaagctattg
    10021 atgatttggt taaggagtta ctgaatcaga atgaaatatg ttttgataca gagacgtctt
    10081 cattagatta tttttcatta aacattgtag gcttatcatt ttcgttcaac agcggaacag
    10141 gttattatat tccttgtcct gaagactttg agaaagcaaa atccatttgt cataaattta
    10201 aacctgtgct tgaacatgct tcaattttaa aaatcggaca aaatattaag tttgatatgc
    10261 ttgtgctgaa acgctatgga gttgaggtga aagctccatt gtatgatacg atgattgccc
    10321 attatttagt tgagcctgat atgaaacatg gtatggatta tctgagtgag acatatcttg
    10381 gctacacacc agtgcatatt gaagaactga ttggtaaaaa ggggaaaaat cagggcagta
    10441 tgcgagatgt tccgattgat aaaataaaag agtatgctgc agaagatgct gacataacat
    10501 ttcagttaaa gcaagtgtta tcacctatgg tcgaaaagca tgaggttgaa ggtgttttga
    10561 aggatattga acttccactg attcctgtat tggccgatat ggaatatgaa ggcgttaaaa
    10621 tagatgtaga ttatctgaat gtatactcta aagaattaga acaagatatg ttgaaatatc
    10681 gttcagctat ttttgacatt gcaggattgg aatttaatct tgattcacca cgacaacttg
    10741 gtgatgtatt attcggacac ctgaaacttc ctgtagataa aaaaactgca acaggtcaac
    10801 attcaaccaa tgaagaagtg ttgagtaaat tggcgcaaga ttatccaatt gccgagaaga
    10861 ttcttgaata tcgtgaactg acaaaattaa aatcaactta cgttgatgcg ttgcctcaaa
    10921 tggttaatgt caaaaccggc agggtgcaca ctacattcaa tcaaactatt gcttctacag
    10981 gaagattgag ttctattggg cctaatcttc aaaacattcc aataagaact gaacgtggtc
    11041 agcgggttcg taaagcattt attgcacgtg acaatgatca catattgttg tcagcagatt
    11101 attctcagat agagttacga ttagcggcag aaattagtgg tgatgagaat atgcttgaag
    11161 cattcaggca ggggcttgac atacatcagg caacagcagc acgggtatat aatgttgctc
    11221 ttggtgatgt aacgaaggaa atgcgaagca aagcaaagat ggttaacttc ggaattattt
    11281 atgctatttc agcatttggt ttatcgcaac gtttgggaat tcccagaaaa gaggctgctg
    11341 aattaatcga gaattatttt gttcagtatc caaagcttcg taactatatg agtgaaacgt
    11401 tggagtttgc ccgccataat ggttatgtta aaacgctcat gggcagaagg cgatatctaa
    11461 aggatattaa ttcaagaaat tttacagtga gaggttttgc cgaacgcgag gcaattaatt
    11521 cgccattaca gggtagtgct gccgatttag ttaagctggc aatgattcat attcatgagg
    11581 aatttaaaag acaaaatctt aaatctaaaa tgacacttca ggtgcatgat gaacttgttt
    11641 tcgatgctca taaagatgag gttgatttaa taaaaccaat tatcagagaa aagatgattc
    11701 acgccataaa aaccagggtt ccattagaag tagaaattgg aattggcaca aattggctgg
    11761 atgcgcatta aatactttca cgtttaaata aaatatcgta cttttggttt gccaaaaagc
    11821 caaaaaaatg aaaattttaa actatctgtg cactgccttt atattattac tagggctaag
    11881 tacacaagca caattaacgg taacgtcagg aaatcctgca gcagctattg ttgatactct
    11941 tgtaggaaca ggagtttcag tagctaacat aacttataca ggcaatgcaa gttctaaggg
    12001 cactttcagg tgtacaggtg gatgtaattt agggattaca agaggtgtat ttctttcaac
    12061 aggtaatgca ggtcaaacac caacttctcc gccatcagat ttccatagta gtgatatgag
    12121 cgcttctggt gatgcccaat taaatttaat tgtagcacct aatctgaccc aagatgctgc
    12181 tgtgcttgaa tttgatttta ccccagcggc agacagtatt ggttttaagt ttgtttttgc
    12241 ttctgaagaa tataatgatt atgtaaacac tgcatataat gatgtttttg ctttttatat
    12301 ttcaggacct ggtattcccg gtagtccaaa gaatttagct cttattccgg gaacttcaat
    12361 acctgtttca ataaataatg taaataatgg atatagtgcc ggagtatctt ccgggccatg
    12421 caatcattgt acatattttg ttgataatgt gcccaacggt ggtactgcta acttttttga
    12481 tggtttcacg actgttatat cagcagggat aaaagttatg ccatgtcaga cctatcacat
    12541 gaaatttgca attgcagatg tgaatgatgg tgtttttgac tctggtgttt ttttagaagc
    12601 taaaagtttt tcaagcattg gcaatgtagg tattacagct aatggtgtgc caattacaca
    12661 aaacctggat acagtttatg cttgcgatgg tgattctgtt gtattgactg cgtcacctgc
    12721 gggaagttat aattggaata atggtcaaac tacgcaaact attgttgtta cgcaggctaa
    12781 catcactcca tcaggtgttt atcaatatgc agtattgaat agctctccgg catgttttgc
    12841 atcaagtaaa caagtaaaag taatttttgt aacaccaaca gccaccatca caccattggg
    12901 ctctactgca ttatgtcccg gaggctcagt agttttacaa gctaaccccg gaaatagtta
    12961 cttgtggagt aatggggcaa ctacagatac tatagtagta tcatcagccg gaacatatac
    13021 tgtcacagtt actcagtttg gcaattgctc tgctgtatct acacctgttg tagttactca
    13081 atcagcacct gttgccatta ttaatggagt attgaccatt tgctcaggaa gtaatacaac
    13141 acttacagca acagcgggta cttcttatca atggtcaacc ggagcaacta cacaatcagt
    13201 ttctgttggt acaagtaatg tatatactgt tacagttacc tattcgggag gatgtactgc
    13261 aactgcatct gttaatgtta ctgccaacac aaacccagtt ccatccatca cgggaaataa
    13321 cagtatttgt caggggcaaa gcagtaccat cagtgcaggt agttattccg gatattcatg
    13381 gtcaacagga gcaacaactt caagtattaa tataaatact gcaggaagtt attccgtaac
    13441 agttacagat gccaatggat gcacaggaac agcaacattt aatcttgcgg ttaatgccaa
    13501 tccaacacca accataaccg gtgtaacttc attttgtgca ggaggtaact caacactcaa
    13561 tgctggagga ggatacacaa attatttgtg gagtggtgga gcaactacgc agactatcaa
    13621 tgtcaccaca ggtggcaact atgcagtaac cgttactaat gcagcaggat gtacagcaac
    13681 aacaaataaa gtaattacag ttaatgcatt gccagtgcca tctgtaacag gcacattagc
    13741 attctgtgca ggtaaaaaca caacgttgac ggtaccggga agttatgctt cttatcagtg
    13801 gtcaaatggt gcaacgacat caaatataac agttaacact gcttcaactt ttactgtaac
    13861 agttacagat gccaatggtt gtacaggaag taaatctgca acaacaacta tcaatgcaaa
    13921 cccagttcca tccatcacgg gaaataacag catttgtcag gggcaaagta gtaccattaa
    13981 tgccggcagc tattccggat attcatggtc aaccggagca acaacttcaa gtattaatat
    14041 aaatactgca ggaaattatt ctgtaacagt tacagatgcc aatggatgca caggtacagc
    14101 aacatttaat cttgcagtta atgccaatcc aacaccaact ataaccggtg taacttcatt
    14161 ttgtgcagga ggtaactcaa cacttaatgc tggtggagga tataccaact atttgtggag
    14221 tggtggagca actacgcaga ctatcaatgt caccacaggt ggcaactatg cagtaaccgt
    14281 tactaatgca gcaggatgta cagcaacaac aaataaagta attacagtta atgcattgcc
    14341 agtgccatct gtaacaggca cattagcatt ctgtgcaggt aaaaacacaa cgttgacggt
    14401 accgggaagt tatgcttctt atcagtggtc aaatggtgca acgacatcaa atataacagt
    14461 taacactgct tcaactttta ctgtaacagt tacagatgcc aatggttgca caggaagtaa
    14521 atctgcaaca acaactatca acacaaatcc tgttccatcc atcacgggaa acaacagcat
    14581 ttgccagggg cagagcagta ccatcagtgc tggtagctat tccggatatt catggtcaac
    14641 aggagcaaca acttcaagta ttaatataaa tactgcagga aattattctg taacagttac
    14701 tgatgctaat ggatgcacag ggacagcaac atttaatctt gcagttaatg ccaacccaac
    14761 accagccata accggtataa cttcattttg tgctggaggt aactcaacac tcaatgctgg
    14821 aagtggatac acaaattatt tgtggagtgg tggagcaact acacagacta tcaatgtcac
    14881 tacatcaggt aattatatag taacagtaac taatgcagca ggatgtactg caacaacaaa
    14941 taaagtaatt acagttaatg cattgccagt accaaccatt agtggtccgg caggtatatg
    15001 tcctggtgcc agtgccaatc ttgttgcagg aagttatgca tcttaccttt ggtcaaatgg
    15061 aaataccaca tcttcaatta ctactaatgt ttctggaaac tatactgtaa cagttacaga
    15121 tgtcaacgga tgtgttggct catcaaatta taatttactt tcttttcaaa accctattcc
    15181 tgttatcaat ggaatggcag caatatgtca aaatcaaaat tcaattttag atgcaggtag
    15241 ctttgcttca tggcaatggt caacaggtgc tgtatcacaa actatatcag tgaatcagtc
    15301 aggtacatat tctgttacgg taaccaataa tgatggttgt gttgcatcaa cttcgtttaa
    15361 tgttgtggtg aatcctcttc ctgttcctgt aatttctgga acttcagaat tttgttcagg
    15421 aaactcttct gtacttaaca ttggtggagg tagttttgca tcatatctat ggaatacttc
    15481 tgcaactacc agtacaatct cagtatcttc agggggaaat tatgtagtaa cagttactga
    15541 tgcaaatgga tgtactgctt ccaccaatca ggtagttaca gtgtggaatc ttccaactcc
    15601 aataatttca ggcaacaatg caatatgcaa tggtacacaa actacattga ctgcaggaag
    15661 ttattcaaat tatcaatggt ctaccggagc ttctacagat tctataactg tttcaaatgg
    15721 tgctaactat attgtaacag taacagatgt taacggatgt acaggaactt cagcaccatt
    15781 tactgtaaca ataaaccaac tgcctactgc aataatttca ggtacaacat ctatctgtat
    15841 tggacagtca actacacttc aggttcagtt taccggaact gcaccttata gctatcgcta
    15901 tagtgatggc acaaatgtgt cagcaatttt aaatagtaac attacagcag cacaaattat
    15961 agttactccg gttagtacaa cagattatac gcttactcaa attagtgatg caaactgctc
    16021 aggcaatttt tcaggagtag caagagtagt tgttaatcca ttgcctcaac ctgttatttc
    16081 aggttctgac ggagagtgcg atggcatttc agccacactt actactggaa actttgcatc
    16141 atatttatgg tcaacaggag caactacaca atcaattgct gttaattctt ccggaagcta
    16201 tgtgtgtaca gtaacttcca cagcaggatg caaagcttct gccagccata attttgaaac
    16261 ttatccactg cctgtagttt cttttaccaa cgacagtagt ctgacctgtg aacttccaat
    16321 aataaatttc actaacactt cgcaatatca gttaggatca gtgtttgaat ggaatattga
    16381 taatacagtt tacaattctc taaatgctac acatcagttt tccggttcag gaagttatcc
    16441 cgtatcatta atcataacgt ctccaaacaa ttgtgttggt gaatcaatag cgaatgtaaa
    16501 cattacagta ttccctttac cacaagcttc atttacaata gcacccgatc aggcaccgtt
    16561 atttaattct gaagtaacgt ttggtgatgt ctctgcaaat gctgtaagct ggttgtggga
    16621 ttttggtgat ggtgatactt caaccttaca aaatgtaagt cacttttatg ctgatccggg
    16681 aaagtacaat gcaaagcttt atgttacaaa cgttgcaggt tgtatagact cattagcacg
    16741 tgaaattttc atcactccat tttatattcc aaatgcgttt acacccaatg gtgatggtcg
    16801 caatgaaaca ttttttgatc cgggttatgc tactaatgtg caaagtctta caatgaatat
    16861 atataaccgt tgggggcagt tggtttatac aacagataat ttgagtaaga cctgggatgg
    16921 agttgattca aaaggaaatc tatctccgca aggtgtatat gtatatgctc ttaaagtaac
    16981 aacttacagt ggtaaacact acgattatac tggtagtgtt tcgttaatac gttaagtttc
    17041 tttacccaaa tacactttta agaatatcat taatacgtgc agcagggtct tttctgatct
    17101 tcagttcttc tgcagctatt agtttaaaaa ggccttcaat cgctttgtcg gtaacgtatt
    17161 gattcaagtc aggatttact tttttcacaa atggcacttt gttataagtt gacattagcg
    17221 gattccagta ttgcgttatc tttactttat taagtgaagt ttgtacaatt ggcttgaatg
    17281 cagcaatgag tgcagcattt gaagtggttt ttaaaaattg tgttgctgca tcattaccac
    17341 ctcttaaaat ggcaagaccg tcattaatag ttatgtgctg aatagagttg atgaaaattg
    17401 gtgctgccga ttttgctgca tcctcggctg cacgattcaa tgtttttaca aatttgtcaa
    17461 cttctttatc catccccata ctacgtaaag tattttccgc atcacgtatt tcacggggga
    17521 aaggtatttt tatcaaaggg tttttataga agccatcaag ttttgatgct gatgctgtgg
    17581 aattattggt gcctactttt agcgcttctc taaggccact aatgatatca ttatttgcca
    17641 aaccttcacc ttgatttgaa ggggtattat ttgttgcctt aaaaatcttt ttctgtgcat
    17701 aagtttgttg ccctataaac acaagtgcaa ttaaaagaaa ccttttcata atctattttt
    17761 ttagaatttt gtttttcaat tcaaacatat tgccattatg tgtctgcaat caatattgtt
    17821 tgtttactta atctgaaatt aatgcagcta aattacttaa ttaatttaaa gttcatttta
    17881 tgataacaaa tgtagagcta attaatattg gtgatgaatt gcttattgga caaattgtaa
    17941 ataccaatgc aacatggatt ggacaacaac taagtctttc cggttattgg ctgaaacaaa
    18001 ttacaaccat tggtgataat gaacaggaaa ttattgatgc tttaaatgct gcaaagagca
    18061 gggcgcaaat tattctcatt accggtggat tagggcctac aaaagatgat ttaaccaagg
    18121 atgtgttgtg taaatatttt aatacacatc ttgtgtttga caagcaggca tatagtgata
    18181 ttgaaaatat ttttcatgca cgcggtaagg tagttacaga aacaaacaag cgtcaggctg
    18241 agttgccttc tgcatgtgtt ccaatttata ataagaaagg aactgctccg ggaatgtggt
    18301 tcaattatgg cgaacatgtt ttcgtcagta tgccgggtgt gccattcgaa atgcaggcaa
    18361 tgatgcagaa tgatgtacta cctaaattaa agcaacagta taaagcaatg cctattgtgc
    18421 ataaaactat tcttacgcaa ggtattggtg agagtgactt gtcagatagg atagcatcat
    18481 gggaaaatga cttacctcca cattttaaat tagcctatct gcctgcagcg ggaatggttc
    18541 gtttacgact tactggtaaa ggtgttgaag acatggagcg agacatgaat cagagaattt
    18601 ctaaactcaa agagctaatt gcaccctaca tattcggtta tgatgatgac actattcaat
    18661 ctgttgtagg gagtcttttg ttggaaaaac aaaaaaccat tgcagttgca gaaagttgta
    18721 cgggaggatt tttatcacat ctcattactt caatcccagg aagttcagca tactttatgg
    18781 gaggtacggt gagttatagc aatgcttcaa agatgagtca gttaagtgta agtgaacaat
    18841 cattgaaaga ttatggtgcc gtcagcgaag ctgttgcaat tgaaatggca ataggagcaa
    18901 gaaaaattta taatacagat tttgcattga gtataaccgg aattgccggg ccgggtggcg
    18961 gaagtgtaga aaaaccgata ggcacagttt ggatgggtat tgctacacac gattcttgtt
    19021 atgccagaaa atttttattg ggtacaaacc gtctgcgaat cattcaggta gctgcagata
    19081 ctgcattaaa tatgcttaga aaggaaataa ttgagcatta ttgaggaata aatttgcaga
    19141 taaggacaaa gtgattactt ttgcaccctc aaaataacaa tgcggggtgg agcaggggta
    19201 gctcgttggg ctcataaccc aaaggtcgtt ggttcgaatc cagcccccgc tactaaaaga
    19261 ttaagctgca caatgtgcag cttttttatt tccttttatt cttattgata gaaaaatttt
    19321 caaaatttat tcccattctt aaaacagagt tgaaatatta ttaatcatgt ctgattttgt
    19381 gggctagatt taaatccgca gaaaatttta tttttggaga aacaaaatca gtattggtat
    19441 gacacattta attaatatca aaagtcgaat ggccatcaag gaatatactt tgctgctttt
    19501 cagaataggt gtttcattgc tgatgcttta cggccatggt tatcctaaat tgcaaaaaat
    19561 attcagtgga gcagaaatac aatttgcaga tccgtttggg cttggcatga agatgtcttt
    19621 ttatttggta gtttttgctg aagtaatttg ttctatattt atcatacttg gattaaaaac
    19681 gcgcatggct tcatctgtat tggtgtttaa tatgcttgta gcaaccttcg tcatggcttc
    19741 taaaggagag gttccggaat tggcgctcat gtattttttc tgttacgtgc tgattttaat
    19801 tttcggagca ggtaaatatt ctgttgacta taaccttaat tcaaagaaga aaggtaagtt
    19861 ttgattttgt gaaaagtttt ttcaagaagg attgaaatcg tgagtattta tttccttatt
    19921 cttctggaat agtttttaaa ctttcccaat caaagccagg taagtaaaaa caaaagggat
    19981 tgaaataaaa agtccatcaa agcgatcgag cataccgcca tgacccggaa gaatgtttcc
    20041 tgagtctttg attttaagac tacgtttaag catactttca atcaaatcgc caaaagttcc
    20101 ggtaattact atcagtaaac taacaatcag ccattctgat aaggcaagct gtgtaaaaaa
    20161 caaagaatta atataaccta aaagcaatgc tgcaagacaa ccgccaatac tgccttccca
    20221 ggtctttttt ggtgaatgtc gttcgaaaag tttatgcttt ccgaatttca tacccgaaag
    20281 atatgcaccg gtgtcacttg cccatagaat taaaaaataa ccgatgatga ttccactatg
    20341 gtagccagta ttttcacccg gcaaaaatgc catagaatta aagagtgcaa gaggtaatgt
    20401 taaataagct actcccagca atgtataagc tatgttttca aatggttttt cagatttgag
    20461 atatagctca aaaatgaaaa taagaaaaag cataggaata tttataaaca ataactgcgc
    20521 agatgccgtc cccgaagcta ctaatgcact ggtagcataa agaatcgcac ttaatacaat
    20581 acctgaaaaa attcttggtg tcaggtcgtt gcggataaac agtttataaa actcaataat
    20641 gctcaacagg ttgatgacca tgaaaagtat catgaatgta taggggctga attcaattgc
    20701 agcaatcata actgctacaa atacagcgcc cgtaaatgct cttgtccaaa agttattcaa
    20761 agttcgttgt cttttataat aaaccgtgca agcacttcaa gtttatcgag tacatatatt
    20821 tcaatattgc ctattaatcc cggataactt gaatcatttt tgttgattac catgctttca
    20881 atatcatgtt cttccaatac agcacgcaca atttcaactt tgtgtggttg tgttgtagta
    20941 tataccttga cccattctgc cggtttgtcc atattcagtt agctcatcat attaagttgt
    21001 tgctctaatg attttatttt ttgttcggca tctgatttct ttttctcttc actggcaata
    21061 acctcaggct tggctttgct gacaaagttt gcattaccta atttcttcat aacagtgtca
    21121 agaaaacctt tgttgtaagc aatttctttt tcaatgcgga gacgttctgc agcagtatca
    21181 atattctgat cgaaaggtat agcatattct tgtgttcctg ccataaaagc aaatgcttta
    21241 tcaaagctat tggctttttc aatgctgctg acattagcta atctaagtat gatattatca
    21301 aatgtagata gatgtttctc ttttgttagc aattgtaatg gagttttagg ggatatacct
    21361 ttctcgtttc gaaaagttct taaagtagaa ataatgtttt gtgcatgtgt cataccttct
    21421 aatatagcat tatctgttga tgcttgtttg ggccacatgc ttacgataat acaatctctg
    21481 taatttctgt ttttaagttc atgccacaac tcttctgtaa taaatggcat gaatggatgt
    21541 agtatctgca gcaggttttc aaaaatatct atagttgtat tataagtttc tgaatctatt
    21601 gcaacactcc cataaggtgg ttttatcatt tctaaatacc atgcacagaa atcatcccaa
    21661 aacaacttgt atttaatcat gagcacttca ctgatgcgca ttttatcata actgtcgttg
    21721 atgagtgtca attgttcatt gaattttgat ttcatccatg ctgttgcaat cctacctgtt
    21781 tcggattggc gcagtgatgc atcaggctgc cagtttttta tcagacgaaa tgcattccac
    21841 attttgttgg caaaattccg gccttgctca caataagatt catcaaacaa taagtcgttg
    21901 ccggcaggtg aagaaagcaa catgcctaca cgaacaccat cagcaccata tttatcaatc
    21961 aactcaagtg ggtcaggcga attgcctaat gacttactca tttttctgcc taacttatca
    22021 cgaacaatgc ctgtaagata gacacgctca aatggaatgt catttcgata ttctaatcct
    22081 gcaataatca ttcgtgccac ccaaaagaaa agtatttcag gtgctgtcac caaatcgttg
    22141 gtagggtagt agtatttaat ctctttgttc gttgggtctt taaatccgtc aaaaacactt
    22201 ataggccaca accatgatga aaaccaggta tctaatacgt catcatcctg ttttatctta
    22261 tctgcattta tttttatgtt tttttcttca aacttcgcca atgcctcctg cggagttttg
    22321 gctaccacaa aatttccgga ttcatcatac catgccggaa tgcgttgtcc ccaccatagt
    22381 tgtcgcgata tacaccagtc tttaacattc tccatccagt agcgataggt actgataaat
    22441 ttatcaggaa tcagtttgat gttaccatta accactgctt ctaatgcagg tttactgatt
    22501 tccggcatat tcacaaacca ttgtaaactc aggcgaggtt caataactgc atctgtacgt
    22561 tcgctatatc ctactttgtt tttatagtct tccgtcctaa ttaagtggcc acttgcttca
    22621 aggtcttttg cgattttctt tcttacaata aatctatcct caccaatgta gtgtcctgct
    22681 gcctcactca ttttaccatc gggtgtcaac gtgtcaatga cttcgagttt atgttttaat
    22741 ccaaggttat agtcgttgat atcatgtgca ggcgttacct taagtgcacc ggttccgaat
    22801 tccttgtcaa tgtagtcgtc aaaaattatg ggaactcttc tgttcacaat aggaatgata
    22861 cagaattttc cttttaagtg tgtgtaacgt tcatcttcag gatgcacaca aacggcagtg
    22921 tcgcctaaaa tagtttcagg acgaactgtt gcaatagtaa tccattcatc agcagtaccg
    22981 tcaattttat atcttacata atatagtttt gagttaacgt ctttatgtat tacttcctca
    23041 tcacttacag ctgtcaaccc tgcaggatcc cagtttacca tacgtagtcc acgatagatg
    23101 tagcccttat tatacaagtc aataaataca tcaattacag cttcacttaa atcgtcttcc
    23161 attgtaaatc gtgtacgatc ccaatcgcaa ctgcaaccta atttttttaa ttgttcaagg
    23221 attattcctc catatttctc tttccactcc caggcatatt ttaaaaactc ttctcttgta
    23281 agatcggatt ttttaattcc tctgtcgcgc aacatctgca ctaccttagc ttctgtggca
    23341 atagaggcat gatcggttcc cggaacccag catgcatttt tacctaacat acgtgctcta
    23401 cggataagca catcttgaat agtgttgttg agcatatgcc ccatgtgtaa tactcctgtt
    23461 acattgggcg gaggaataac aatagtgtag ggtggacgct catcaggaac tgacttaaaa
    23521 aatttttcat tcatccaatg tgcataccat ttggattccg tttcctgtgg gttatatatt
    23581 tttgataatt ctgacatgtg caattttaat gtgaatgcaa aaataagaaa ttagccatgt
    23641 gccaaacgaa caaagcctgc tatccgggca ggctttgaga aataagtaaa tattcttatt
    23701 attaccggaa gattaaacta ttctccacct ccgattcttt tttgttcctg ctcactgcca
    23761 ctactgcgct gtctgatatt tttcatctgt ttattcccaa aacgccaggt aaaagacagc
    23821 ctgagttgct gactttccca attgccccat gcacgactca caatacctgc ataagtgttg
    23881 tagctgtccc atggtgttgt atgcaataag tcggtatatg aaatttttaa tgttgcttgg
    23941 tctttcaata attttttctg aagacccaca tctaatgatc cctgtgcctt ggttttaaat
    24001 gatccacccc aaacacctcc ggagttgtac catccagaga gttcaaatgt aaactgataa
    24061 ggcagcttaa atgtattttg tccatataga ttaaacgaag taaactgtgc gtttatggtt
    24121 ttattaccac catagtctgc tttgtatttt tgattgtcaa cacttgcatt taaataaatg
    24181 ctgtaccatt taagcggctg tagtgatgca cttaaactaa cgctcaatac atcttctgta
    24241 gccagattac gaggcatcaa ataactcttt ccttctccaa gtgtgtcagt aatctgtgca
    24301 aaaaagtctt ttgtgtgact gtatcctgct gatgttgtaa tgatatattt ataagtgtgt
    24361 gtaagttcta ttttgttaga atactgtgga tctaaaaatg gatttccctt tctaaaactt
    24421 aattcatcaa gtttgtattc aaatggattt aactcctgat aattcggtct gtcaattcgc
    24481 gaactgtaaa ttaatcctgc agaattgtcc ttgttgatgt tgtaagtgat tcctgcgctg
    24541 ggaaaaaaat ctgtatagct gcgttttaca tccttatcat ttgctgtagc agaggttttt
    24601 aatttaccat tactgtttgt gttctccatt cgtacaccta tttgcacatc aattttattg
    24661 aatgtgcgtt ggtaatttaa atagagggca ttgacatttt ctgcgtaatc gaatttgttg
    24721 ctacgagaag aatccaatga tgcaataccg ccttcaatgt tgtaaaaatt aaacgtgttg
    24781 acagtgttta ccaaagaaac cttatacccc gcacctaatt tgcctttgaa aagattttga
    24841 gtataatcag tttttaaagt atagatattt attgttgttg gtgtgtttga cttgtagtat
    24901 gttgcagaca gcggtgttgt tccatcgggt aatgtgtatt cattaggtag ataggtgttt
    24961 cttgttccat tgtagtagcc atagtcaaaa tctgtagtca attctttgcc tgttgtgtct
    25021 ttgtaatgat gatttaaatt ggcataaaaa ttattgttta ttgaattgta gcgttgatta
    25081 gagttaagaa tgctgtcggt tactcctgaa taaaaattgc tgatgaagtt tttatttttc
    25141 tgacgacctg taatgtcact ataattacca ttaatcatga ttccaaaact attattagcg
    25201 ttcatttgat agtcaacacc gactttgtaa ttatgactca aaccaccgcg cttggattta
    25261 gacgattgat tgaaggtaaa agggttttgt tcgcgatata gatagaattc gttttttctg
    25321 tcaccccagt tattgctgta agtagagtag atattaaact tttttgttct gttgttgact
    25381 gaaagcgagg tgttatattt ggaatatgtt ccaacggcat aaccggctgt tatgcttcca
    25441 ttggtgccat agtttttgtt tttctttagc ttgatattga tgattcctgc tgtgccctga
    25501 gcatcatatt tggatgatgg gtttgaaatg atttcaatac tctctacatc tgtactttga
    25561 atagaacgaa ggtaatctcc aagttcgctt tcagtcatcc ttatttcacg tccgtcaatc
    25621 tgaaccatca cgccaccacg accttttagt ataatgttat cgttgttatc cacaacaaca
    25681 ccgggtgctt tttgaagtaa ctcaagtgca gtacttcctg ttgagttgat actgttttct
    25741 acattaaata cagttttatc ggctttaact tcaaccaaag gtttaaatgc tgtgatggtt
    25801 gcttgtttaa gattggttga tgatgaagaa agtttaattg tgtgaaaatc tttattatca
    25861 ccttctttaa agtcaaatgc cggacttaaa taattgttga atccgagaaa tgttgttttt
    25921 agaaaatagc ttccgggttt tatctgttcg aagattacct ttcctgaaac atcagccaca
    25981 gcaactttac tcagcgttga atcttttatt tgccggagtt ctgcaatggc acccgttagt
    26041 ggttcgttat tgttgtctgt gattttcgcc tgaatagttt gactcattac aggttgtgta
    26101 agcaaggtta ttcccgctaa aatgtttaga atagatttca ttgtttgtgt gttatggttg
    26161 cggtaaaaat ttattgttac tgtttggtgt tatttaattt tctgttgaag tttgattttt
    26221 tcttaattgt aaataatcga gataatagag taccttaatt gaaaatgttt gaacatgtgg
    26281 atttttaaag acgtaatcaa gatttttagt gaacgatgca gtttctaatt tgccatcagt
    26341 gccattatca aagatgtttt tgtatgaaaa agtgagggtg ctaccgggag caaaccgcca
    26401 agaaaataga agatcggcat taaaaaaatt atagctgcca tcaaattcat tgttgtaaat
    26461 atctgttgta ggtgaaagtc cgccatcgtc ctgaaggaaa aagaatttcc tgtatgcgta
    26521 attaaaccaa taatgtcttg caacaataga aagtgacaga tcatttttaa atatatagcg
    26581 ggcagcaaga ctgttgatat aggtattggt tcttcttaac ccaaaaagat aaatattcgg
    26641 atctgtttca ctggtaaatc caaaattata tggatcaaaa ttgtattctg atgaagcgtt
    26701 taaagtgagc ttgtcattta ttcgatagcg taaagttgca tcaaaatttc caccttcaga
    26761 tagataggtt ttaataaagt tacttatatt aatttccata tctagtgcta atgcctttcg
    26821 gtaatctgta cttacaccaa taaaagcaaa ccagtatttt aaagcattaa catatcttcc
    26881 atttaagcga gggtcatatt cgcgtccggc tgctggtgta aatccaccac cgccaaaaat
    26941 tgcagtgtat gatttaaaga gtataaaaag attggtctgt aaagaagttt ctctcatgta
    27001 gccggttttg taatgttgag agtagtttaa atcaaagcgc caattacctt cctgcacagg
    27061 accataaggt ttgaattgga aaaatgtaat attagcattt gaattaattc tgtttggtgt
    27121 cacatagtaa ccaaaatctg ttgggtcata agtaggattg acgccaacct gcgaaagccc
    27181 ccaacgaatt gttccgctgg ttttttttat tgcaagataa tgtcgtaaac catcagtctt
    27241 tttatcagag ttgattccat caggatctgt aataaattta atactgtaat caagtccgcc
    27301 actgagacta taggtatttt ttttattcac taatttaaat ccacctcctg taacatttga
    27361 atcactgaac tttttagttc tggttacatt ggtgtttatc agataaaccg aagagttgct
    27421 tttccattgt ttatctgcaa ctattacatt atagtttgca agtggttctg taaggatttt
    27481 ttccgttagc cctgtgtttt tgtctttgat tattgcatag gtgttgtttg ttatagcatt
    27541 aaaaaaacca aggccaatgc cctcattggt tcgtccggaa agtttggttg cattaatcag
    27601 ttttgattct gttggatttg atttaatatc actttcatca tatttattca tcaagtcata
    27661 atatcgtgat ggtgtctgac ctattcttct gctataaaaa agattatctc tggaaaaaat
    27721 atctgtacct tctttaaaga actgacggtt ttcatcatag tttatttctt cgtagctaag
    27781 tgtttttctt ttattgtcag actgtacttg tccgaaatcc ggaagcaagg tcatgtctaa
    27841 ggtaaagcgt tcatcaaggc catatttaat atcagcacca ccacgataac tgtaagtttg
    27901 atttgctttg gagccggaaa cgggttcgtg ttcgtatgct acactaatgt atggtgtcag
    27961 tgatagcctg agtggaggtt taatgttttc aataccatcg agcacacccc aatagttttg
    28021 agcattagct gctgttgaag gtgtcagact ccattgctga aattctctgt tgcgccttat
    28081 gtttcttgtt atctgaaaag cccattcctg aattggtttc gatggaaatc gaattgcaga
    28141 ataggggatt ttaatttcaa cacaccagcc tttatctgtg atttttacag aactttccca
    28201 aacagcatca aacaaataat cgctataacg actgtcggct tgaattcctg aagcatacac
    28261 tccaaaatcg tatgcatctt gccgcgtatt atacggatca aaaacaaaac ggaagtaatc
    28321 tgcattgatg tctgtaaaac cactgttgct tgatggatca gtaccatcac gcagtcctaa
    28381 ttcttttaaa atactgtcag gtgcagagtc ataaagcata gcacctatat aaattgctgt
    28441 gttgtcataa acaatacgaa cttctgtttt ttgtgtcggt acaccacctt caatcgggcg
    28501 gttcatcaca aaatcgctta atgcattggt gcttgcccaa acagattctg ttaactgacc
    28561 atcaattttt attggttcaa aaattcgtgt tgctttataa tgcttaagac tatccgaggc
    28621 gctaacaaca cctgaaaaaa gtacagaaag aaaaaggaca tgaatgacct tgtgcaacat
    28681 acgggagtaa ttagcggcaa aactataact acatcgaggg cttgaagtta agtaaaagtt
    28741 aatcagcgtt aaagatgtta atattaattt attctctgaa aattactgac ttgttttttc
    28801 aattcagagc agtagatttg cctcataagt tttaatcgtg aaggtaagtt taacagcccg
    28861 gatatttata ggaatgttac tggcaatttt gctgggacat tggtacaata tcagccacac
    28921 agcagccgaa ctggatgttt ttgcttccag aataagcata ttgagtgata tctttcttcg
    28981 attgattaag atgattatcg caccattggt ttttgcaaca ctggtagttg gcgttgccaa
    29041 agttggcgat tttaaaagtg taggccgcat aggccttaag acaatcttgt attttcagtt
    29101 tgcaactatt cttgcacttc ttttgggatt actcttagtt aacttttttg aacccggcaa
    29161 gattatgaat ctgcagatac ctgcacaatc ggcatcctct ggagtctcag caaagggcct
    29221 taatgctaaa gattttataa cccatgtcat tccgaaaagt attgtggagg caatggccaa
    29281 taatgatata ttacccattg tggtttttgc cttgtttttt ggtttagctg ccgcatctgt
    29341 tggcgaaaaa gccaaaatgg ttgttaatgc aatggatgca gtttctcata tcatgtttaa
    29401 agttacaaat tttgtaatga agtttgcacc ttatggtgtg tttggggcag tggctgccgt
    29461 tgttgccaag cagggactca gtgttatttc cggttatctc tatttaatag caacttttta
    29521 tggaggtctg cttttctttg ggttagtgat attgggattg atttgttatt ttttaaaaat
    29581 cccatttgta aaactattac aacatatccg tgagcccatc ttactggcat tctcaactgc
    29641 gagctctgaa tcggcatttc ctaaaactat tgaggcattg gaaaaatatg gctgcagtaa
    29701 tcgcataatt agttttgtat tacccttagg ttattcgttc aaccttgatg gaagtattat
    29761 gtatatggca tttgccactc agtttattgc tcaggcttat ggtatggatt tgaatattca
    29821 gcagcagata atgatgttgt tgatgttgct tgtaacaagc aaaggcatgg caggagtgcc
    29881 aagagcaagc atggttgtta ttgccggaat gcttgagtca tttaatattc ctgaagcagg
    29941 attgttgttg attcttggag tagatcagat attagatatg ggtaggtcag ctaccaatgt
    30001 agtaggcaat gctgtggcta cagcagtagt gtctaaatgg gagaaagagt tataacgttc
    30061 atactttttt cacagcctaa gtctaacttt ggcgcattgt ttgtcgttaa agagtaaaat
    30121 aatttaactt tacagcacaa ttaaattaaa tcaaaatatg aagaaattat ttttagcagt
    30181 agtgattttt tgctcagcca ctatggtaaa ggcacaagac gattctaaag ctgcaccaaa
    30241 acctacagct acacctgcta tcagccccaa tgctgccgac attagttttg aaaaagaaac
    30301 gcatgatttc ggtacaatcc cttataatgg caatggtacc tatgaattta aattcacaaa
    30361 cacaggtaaa gagccattag ttatttcaaa cgcaaaagga agttgcggtt gtaccgtacc
    30421 atcctggcct aaagagccaa ttttgaaagg tcattcaggt gttatcaacg tgcattatga
    30481 cacaaaacgt ccaggcccgt ttacaaaaac agtaaccgta tcatctaatg gtaaaactgc
    30541 cgaaaaagtg ctgacgatta aaggcaatgt tgagactaaa gaacagacag aaagcggaac
    30601 tccggttaaa aaagataata ccatggctcc aaaggagacc actacaaatc catttggtaa
    30661 ttaatcacaa tttatttaaa atataataca cacaaatcaa caatcaaatt tcaaaaaatg
    30721 aaaaaaacaa ttcttctttc agcattagct atcggtatgg caactgctac attcgcgcaa
    30781 gataaagcag cagctgttca ggttgctcct gcggaaaaca aaaatcaagc tgactttaag
    30841 tttgaaactc ttgagtataa ttatggaacc attaaacaag gcgacaaagt taattacacc
    30901 ttcaactttg taaacacagg taaagagcct ttgattatta ccaatgctca aggttcatgt
    30961 ggatgtactg ttcctgagtg gccaaaagag ccgattaaaa aaggtgaagc aggcaaaatc
    31021 catgttactt tcaattcagc aggtaaaatg ggtcttcaag acaaaacaat cactatcact
    31081 tctaatgcaa aaaccagtcc tgtagtttta catataaaag gaaatattga ggctgctgct
    31141 acgcaggaag caccaaagaa ttaatcgagg ttaaaggatt aattataatt ttgcagggac
    31201 gattaaattt aatcgtccct tatttttttg tatgtaatta actaatacaa taactgcatg
    31261 ccatctattt cagaaaaagg caaacacatg ccgccatcac ctatcagaaa attggttcct
    31321 tatgccgata aagccaaagc agaaggtaaa aaaatatatc acctcaacat tggtcagcct
    31381 gatatagaat cgccaaaggt gatgctcgat gcaatccgga acattgacct taaagttatt
    31441 gaatacagtc attcggcagg gatagaatca tatcggagaa agttgatttc ctattacaag
    31501 aagtataata tcaatattaa ctttgaagat attttaatta ctaccggtgg ttcagaagcc
    31561 atagaaattg caatgatgac ttgtctgaat gaaggtgatg agattattat tccggaaccg
    31621 ttttatgcaa attataatgg tttttcaaca caggcaggag ttactgtagt acctgttttt
    31681 tcatccatta gaaacggatt tgcacttccg ccacatgatg tgttcgaaaa ggtcattaca
    31741 cctcgtacta aagccatcat gatttgcaac ccaagtaacc caacagggca tctttattca
    31801 aaagaggaat tgcaaagttt gcgtgacctt gctttaaaac atgatttata tctttttgct
    31861 gacgaagtgt atagggaatt ttgctacgat aacaatagct atttttctat tatgcatctg
    31921 gaaggattgg atgagaatat tattttgttg gattccatca gtaaacgata tagtgcctgc
    31981 ggtgcacgaa ttggcgccat gatttctaag aataaaaaag tgatgagcac agctatgaaa
    32041 tttgcgcaag ccagattaag tccgccaaca tttggacaaa tagctgcgga ggccgcaatt
    32101 gatacgcctc aggaatattt tgataatgta aaagcagagt atgtggcaag aagaaatttt
    32161 gttatagagc agttaaatag catgccgggt gtattttgtg ccaaaccaag tggagcattt
    32221 tattgtatgg ctgaattacc tattgacgat tcagatactt tttgtcaatg gttgctcgaa
    32281 aagttttctt ataagggtca gacagtaatg ttagcacctg ctaccggttt ttacagtcat
    32341 gccgagcttg gaaagaagga agtgcgcatt gcttatgtgc ttaatcttga tgatttgaaa
    32401 aatgcgatga tttgcctgaa cgaagcactt aaggtttacc caggcagagt ggaaaacatt
    32461 tcgaataata ctatcgttat gcaatagcat caggatgtgc gtgcataata aaagtgaata
    32521 gacaaaaaat atgattccag actaccaatc gctaatgctt ccattaatga agcttgtttc
    32581 ggacggacag gaacataagt atcgagactt gattgaaaag ttagctgtcg aatttcaaat
    32641 aactaacgaa gaaagaaagg aacttttgac aagtggaaat caagcaattt ttgacaatcg
    32701 ggttggttgg gctaagacat acttgaaaaa ggctggactt cttgactcgc caaaacgtgc
    32761 gacctttata atcacagacc tcggtagaga gactttatca aaaaatcctg accgaataga
    32821 tgcaaagtat ctaaaacagt ttcctgcctt tatggaattt ataaaagcat caagaaacaa
    32881 caatgaaagc gaagaagatg aaacgtcaat aattgagaat aacgaacaga cacctgaaga
    32941 gagtttagac aaagcatatc agcgaatcag aaaatcgtta gcctctgaac ttcttaacaa
    33001 ggttgttgac ctctctccgg ctttttttga acgacttgta gttgagcttt tagtaaaaat
    33061 gggttacggt ggttcaataa aagatgctgg aaaggcaatg ggcaaaagcg gggatgaagg
    33121 aattgacgga acgattaaag aagataaact cggacttgac atcatttaca tacaagccaa
    33181 gcgttggaaa cctggtaatg tgatcggacg acctgaactt cagaaatttg ttggtgcact
    33241 tgccggacaa ggagctaaaa aaggaatttt cattacgaca tccaatttca caaaggaagc
    33301 attagactac actccaagaa acgaaactaa aattgtgctt atagatggtg agcagttggc
    33361 tcagcttatg attgattaca atcttggttg tacaccacaa cagacttatg aaattaaaaa
    33421 aattgacagc gactattttg gcgaggagta ataaagaccg aaaagaaagc tgaccgcata
    33481 acatggtatt gtcaaaagtg gggctgaatg gatgcgattg gacgttctca caaaattcaa
    33541 cattctaatt cctaccgaac atttatacta aatatcccat ctttcggtac aacttaaatc
    33601 gttaatcagg gctaacaaac acgatgcatt agtgatttag taaaccacaa atgcattgaa
    33661 tcaaaataga agatttattt cctgatgtta atcttgactt aataagcttc ttatagaagg
    33721 atatgaaaat tgcattgctg catttgcttc cgctttagtc atccatttaa cttctgtaat
    33781 actttcctcg gtttgtggta aaagatttgt tgttgacgat gtagtcattt cgaaccaata
    33841 agttcttttt aaaatatatt ctccattttg atgatagata tgataggtgt ccgtcaaggg
    33901 tttaataatt ttcagattgc aaactcctgt ctcctcacac acttcgcgca tggctgaagt
    33961 ttcaggtgat tctccatttt ccatttttcc ttttggcaaa tcccatttac ccaatctgtg
    34021 aattagaagt aacttttttt catgattata taccactcca ccagcagcag caatgtatgt
    34081 aaacaatttt ataaaatccc ttacacctga ttcagcattg tcgcacaaac aaacaatttc
    34141 catgcttttt gaatctgaaa gaatttgctt aagtttatct ttatcaatgc tattttcaaa
    34201 ctttatcaca tcatcagaaa aactatcagc attgtttgtg aagtaaaaag ttttattgcc
    34261 tgcaaatatt ttaatcatgt ttgatttttt attttaaaaa ataaccatta ggtttgtgct
    34321 gaatgaaaga tgacactgct tcaaatatag cagatttttt attgcagatt aaagcaataa
    34381 agcttcagcc tgatgagcct tttacatggg catcaggatg gaagtcgcca atctattgcg
    34441 acaacagagt tgctttgtct aatccaagaa tcagaaccta catccgccag gagttggtta
    34501 aagctattga acgaaattat ggacgtcctg atgctattgc tgccgtagct acaggtgcaa
    34561 ttgcacaggg tgctttggtg tcagaaagca tggggctgcc atttatttat gtcaggcctg
    34621 aacctaagca acatggtgcc aagaatcaaa tcgagggaga tatttctcag atacattccg
    34681 ttgttgttgt tgaagatctt atttcaaccg gcaagagtag tcttaatgct gttgcagcat
    34741 tgcgcaacag cggcattatt gtaaaaggaa tggtttccat ttttaattat ggctttgata
    34801 ttgccaggga gagttttaaa aatgcgcaat gtccattgta ttcattaagc aactacgatg
    34861 tgatgattac aaaagcattg cagtcgaaat atattaacga aggccagatg gaatcattaa
    34921 aggattggcg cgagcatccg gatagctgga ataaatagta aaagaatgac aagaatagaa
    34981 agtgatgagt gcgtaataga caaacctgcc aatgaggtgt ttgcgttttt aagtaattgt
    35041 aacaacctgc agcaattaat gcctgagcaa gtaacaaact ggaaaagtac aaatgatgat
    35101 tgtagtttta caattgcagg catggcaagc cttggcatgg aaattaaaga aagaaacccc
    35161 gatcagaata ttcgtgtgga acgtaaagga aaagcacctt tcgattttgt gatgaattat
    35221 gagatgcaag cagtggagga taataaaaca aaattaaaaa ttgcttttga tgcagatttg
    35281 aatcctatgt taaagatgat ggcagaaaag cctttacgta acttcctgaa tacattagcc
    35341 ggaaattata aaaacttaaa tcagtaattt ttttcagatt aattcgtgat gcgcaatatt
    35401 gtgccatcat aaaatgtagc atactgaacc aatgcatgtg tagcaggccc ttgcgaaata
    35461 ctaccatcgt aaatcataaa ctttgaacca caacaactgt cacgcaaagt aaaattatca
    35521 ctcattacag tcacaccgtc acaactactc tctgcatcgt aagtgcaggc acgttcatag
    35581 gctacaaact cgttaatgct gcgtctgtaa atgataattc ccttgtatcc tgccggataa
    35641 taaatgtaat tacctacggc attcagttgg actaattgtg ggtcgttggc ataaaagata
    35701 acattgacat atgcataagg cacaatgccg ttttcttttt tgcgacaacc ggaaaataaa
    35761 aaaatcaatg tagaaatcag aattatattc tttaaacgca ttacttttga agttaatctt
    35821 ccaaaattac aacataattg ttttaccatc gttaaaattg aagtttagtt aatatgggaa
    35881 agtacctgaa aagatttcta atgttattga tgcttgtgat aagcacgact gttgtatttg
    35941 cacaaagcaa ggcaagcaag gcacaacgca aagccgagaa gaaaaaggag atggagaaaa
    36001 agaaggcatt gaaggcagag gataaagccc gtaaacacca catgaaaata cagactaagg
    36061 aagtgcgtaa gcgaatgaaa aagaatagaa agaaatcagc acgcgaaact ttcagaaggt
    36121 ctgaaagcta ctaatacaat atcaggcttc tatggtgtct gcatattcgc gcaggtcaac
    36181 aggaacaaca gctgtcacac cgggctcatt catggttatg ccataaataa tatccgtagc
    36241 agccattgtc tttttgttgt gtgtgatgat aataaactgc gaatctttag aaaatttctt
    36301 gatgatatta ttgaatttat caatgttgtt atcatcaagt ggtgcatcaa cctcatcgaa
    36361 gatacaaaaa ggtgcaggct taactaaata caatccaaag agaagagcag ttgccgtaag
    36421 tgttctttct cctccagaaa gctgctgaat ggaaagtggc tttttgcctt taggctgtgc
    36481 aataatttca atatcggact ctaaaggatt gccggtgtca gctaaaatca tatcacaatt
    36541 gtcatcagca gtaaacaacg atcggaaaac attgataaag ttattgcgaa cctgattaaa
    36601 tacttcactg aatttttctt tggcagaatt gtcaatctcg gcaatggttt gaagtaaggc
    36661 atcttttgct tttttcaaat catcctgctc ttttatgatg aagtcataac gttcctttat
    36721 ttctttaaac gaatcgagag ccatggggtt aataggtccg aagtttttca actgtccacc
    36781 taatttttca ttccttacct ttaaatcttc ttcattccat tccggattag cttcttgcga
    36841 aacaatatct tcgacttcaa gtgaaaattc tacactcaat ctttctttca atgaagtcat
    36901 tttcagattt acatcgctca cttcattatg taaagcatgc atcaactcat ctgccatctc
    36961 acgtttgcgt ctggtttcag caatttgttt gtcaaactga tcaatctttc ccttagcttc
    37021 aaaataattt ttttcacttt ctcccaaacg actttcaagc aacacttttt cttcaacctg
    37081 tgttttcagg tcagtttcag aagagttgcc tgcctgtgct gtatcattta tctgctcatg
    37141 aagttgttca atttcggttt tgattttttc tactgatgat cttaaagaaa ctacctgatt
    37201 gtttttatag ccaatctcac gaatgatgtt ttgtactttg ttttgttgct gaagaaatgt
    37261 aatatttttt tgattgaatg cattggaggc atcctgtgaa attttttgta gctgccccga
    37321 ttgattttgt ttttcccttt gctgatctgt gagcttttgc agatttattt tcatctcatc
    37381 aagtgacatg ctgctaaacc ccggagcatt atttttctgc tcttgtgtta actgaagttc
    37441 ttcttgtaat ttctgcagat ttttttcatt actctcgcaa ttagaagtga taaaatcttt
    37501 cttgcttaac agtgtattta tgtttaatac tgccttgctt agttcactgt ctgtaagtga
    37561 aattattttt cggttttcgg tagcttgtgt ttttccggct gacaacagct cttcggtttg
    37621 ctgtaactca atctttaata cttctgtctg ttgttgatgt tgttgtattt ctgccaatag
    37681 cttctcaaga tgtttcagtc gtccggtgcg tttgccgtca taacttccca cagagccacc
    37741 actgatagca taacgttgcc tgtaaaattt accgtcaggc gaaagaatta ttttgccatc
    37801 aatttcattt tgatttgcat gtgtagcaac atcattgttt tgtgcaatat atacgttcgc
    37861 caaaagataa ttcatcagcg actgaaagcg gccatcattt tcaattacag tcgaagcagc
    37921 aatgcaacct tcaatttgat gagttgatgt ttgctgataa cttgtaaaag aatctaaaat
    37981 aaaaaagtgc gcacgacctt tgccttcact atttaattta tttagaccgt tccatgcctg
    38041 ctcgcttgac t
//
LOCUS       DAOQNP010000020        36169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_376_length_36169_cov_8.091682, whole genome shotgun sequence.
ACCESSION   DAOQNP010000020 DAOQNP010000000
VERSION     DAOQNP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 36169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36169
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_376_length_36169_cov_8.091682"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..2504)
                     /locus_tag="PKY09_04515"
     CDS             complement(48..2504)
                     /locus_tag="PKY09_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNB12151.1"
                     /translation="MKEFKIEEILPLEGVKSKIWIMFDVLRSENISSDDYHVVLFLLS
                     AYKDDLISIDLVNENHDLKERLIERLRNSNSELDNQYAPIFQGFKPSIQKLSNNGIRN
                     FFRVLAEINKQVLTENFPDVFDSVLYRISQSQGRYAGEFIQPVELTRFMCGLADLKRN
                     AKVFNPFAGLASFGVNLDQGQDYFGQELNQKTWALGALRLMAYERPGNSKYVCDDSIL
                     NWPDESEKFDLILSNPPFGMRLGRHYRDIEPNIRTIEQFLIEKGVQSLKQNGKLIALL
                     PQGFLFIRGMYEQRLRKHLVEEDLIDTIISLPGGLLLNTGIPLIILVLDRNKKLPGRV
                     KFVDAKNFVIAKGPREKVLNDYSLNSYIHNNKEDNNVVKIVYNEQIRDNDYNLSVARY
                     FQKQIDGVKLGDILELVRGQRGNLPETGKLIRIRDLKDDKVDFTLDVSSVEETELRRP
                     DINLVSESCLLLAMRWRTLMPTLFEFKGEPIFRSQDILSFKVNEAIADKAYLIYELHA
                     DYVQEQLESYRLGATIPIIRKEDLMEIVIKLPSIEEQRAKIQGIYELSDKIKRLQEER
                     NALAHGKNRKQFNEFASLKHTLGRPRQNILDWSDNLLEFLTKKKEGFEALNKAFSEFY
                     DIDIISALKEIKRDVNFITDVLEKGENGLVLSKYEKKVVPLSDINSIINDLSNNGFKF
                     KIKKLLLKGEKLKERGIYANKTLFKTLMDNILTNANKYAFDKKATGNDVVIELTEVDD
                     SLAMEIRNNGKPFPKNFDREKFISKYSTADSSNGSGLGGYDIHRIASDFNNPDWILSL
                     NEDPFFLVKFKFQFPIKLVN"
     gene            complement(2569..3579)
                     /locus_tag="PKY09_04520"
     CDS             complement(2569..3579)
                     /locus_tag="PKY09_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNB12152.1"
                     /translation="MATNKHATIRYHALDRCFSNYGRKFFIEDLIIACNEAIYEFAGV
                     EDGVKRRQVFDDITFMESEQGWSIPLQRFKEGKRVYYRYSDKSFSIKSQGINQTEAEQ
                     LKETLTILTRFKGLPQFEWIEEMQIRLEDTFKLEGNNQSSVGFEQNPFLKGLSHFTGI
                     FNAIQYEKALKISYQGYRQETPTQIILHPWYLKQYNNRWFLFGLNEDYKSITNFALDR
                     IIEFEESKTKFQKNEKVNFDEYFDDVVGVTVKRDAPIEKLLIKLSKEIWPYIESKPLH
                     GSQKIKSRTETEVLIELVVQINHELISLLFSYMDAIEVIEPDSLRERFKRLTETVYKK
                     YI"
     gene            complement(3581..3751)
                     /locus_tag="PKY09_04525"
     CDS             complement(3581..3751)
                     /locus_tag="PKY09_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12153.1"
                     /translation="MTDNGNDIQTILTKRTDLQTDRVLLFGQVVENKEKADPSAKPKE
                     PFFANPLSTSIF"
     gene            complement(4083..6218)
                     /locus_tag="PKY09_04530"
     CDS             complement(4083..6218)
                     /locus_tag="PKY09_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB12154.1"
                     /translation="MIKPLLSVLTITSIMISCKPASEPATSEKLTYPITAKSDQVDDY
                     FGVKVSDPYRWLENDTAEDVKKWVTEENKVTFGYLEKIPFRNKIKERLTDIFNYPKYS
                     NPFRAGEYYFFSKNDGLQNQSVIYYQKGLDGKPEVFLDPNTMSSDGTAAVSLLGFSKD
                     KKYVAYAINQSGSDWQTIYVMEVATKKLMSDKLEWVKFSGASWKNNGFYYARYDAPPK
                     GKEFSQKNEYHKIYYHRLGDTQDKDVLVYENKDKPLRYYGASVTEDERFLIIYVSEGT
                     DGTEMYYKDLQSNQKDFGLLFAGFQDNYSVINNIGDKLLVQTDAGAPNQRVILVDPKN
                     PKKENWKDVIPEKPELLESAATGGGKLFVSYLKDVTTHVYQYSLEGNLEHEIALPALG
                     TAFGFSGDKEDALIFYTFTSFTYPPTIYKYDIASGKSDIWQKSDVKFNPEEYETKQVF
                     YNSKDGTKVPMFLVYKKGIKLDGNNPTLLYGYGGFNISLTPSFSTSRLILLENGGIFV
                     MANIRGGGEYGEKWHNEGKLLKKQNVFDDFIAAAEYLIKEKYTSSSKLAIQGGSNGGL
                     LVGACMLQRPDLYRVAFPAVGVMDMLRYHKFTVGWGWAVEYGSSDSLENFKNLYAYSP
                     LHNIKEGVEYPATMVTTADHDDRVVPAHSFKFIATLQERYKGNRPQVIRIDVKAGHGA
                     GKPTSKIIEEQADIWSFMFYNMGITPIYK"
     gene            6307..6612
                     /locus_tag="PKY09_04535"
     CDS             6307..6612
                     /locus_tag="PKY09_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNB12155.1"
                     /translation="MFVYNVTVNIDNDVAAEWLQWMQEIHIPDVLNTGCFIENRIFRV
                     MADEDSGGKTYSVQYYFRTMEDMERYQNNYAAELQQEHTARYKEKFVAFRTLLEVVC"
     gene            7062..7739
                     /gene="ung"
                     /locus_tag="PKY09_04540"
     CDS             7062..7739
                     /gene="ung"
                     /locus_tag="PKY09_04540"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNB12156.1"
                     /translation="MESSQIKIEESWRKMLQEQFNMPYFEELKKFVKQEAALYKVFPP
                     GPQIFSAFDYTPFEKARVVIIGQDPYHGAGQANGLCFSVSEGIKPPPSLVNIFKELKS
                     DLNIPIPVHGNLEKWARQGVFLLNATLTVRQSMAGSHQGKGWEQFTDAVIRTLSEHKS
                     GLVFLLWGRFAQAKEILIDNKKHHILKSAHPSPFSATKFFGCKHFSATNRILKSEGLQ
                     PIDWNIN"
     gene            7796..9469
                     /locus_tag="PKY09_04545"
     CDS             7796..9469
                     /locus_tag="PKY09_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNB12157.1"
                     /translation="MQLSFAQYRLELKGDVVSIKYKKEFADSVLLKKEITKIINEAQK
                     EGFIAASADSLAKDGNIYSVYLHKGNRYKWARITGGNIDERVLNETGNSEKKWRGSKV
                     SPPRMAQMFKSLLHWYENNGYPFAEVKLDSLNFNEQQISGVLRVNKSELVRVDSIIIR
                     GDSKLSRTFLFNYLRVSQGDVYNESIIRNISIRLKELPMVSEYAPLVVAFEGGGAVLT
                     LFLNNKKASQADGIIGVLPDATKSGKVNISGELRLKLLSAFGRGEQFEINWKQPLPKT
                     QDLKTSLSYPFLFSLPFGLEGGLNIYKRDTSYVDVLLKGGIQYLMKGNDRITFVVSNK
                     KSSLLSTSSYKNATVLPAFADASITTYGVVLKKEKVDYRLNPQRGFMAEISGGAGVKK
                     IIKNSSINSELYKGIKLNSDIYKFEGAADFYQTLLPRIVLNTGIAFAWMHADDIFQND
                     QFRFGGLKSLRGFDEDVLYATSYYLGKIELRYILDRNAYLQLFYNQAYYASETRAGNM
                     YDSPFGMGAGITFETKLGIFALNYAIGSQQNSAIQFRQAKVHFGIVNYF"
     gene            complement(9623..9844)
                     /locus_tag="PKY09_04550"
     CDS             complement(9623..9844)
                     /locus_tag="PKY09_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12158.1"
                     /translation="MYESSTTHDLILYYFNEVELSDTVLIQHEIDCNPETEESYQEIV
                     QVMDHIDQCMVNPSSSVIKNILNYSHSLK"
     gene            complement(9911..10495)
                     /locus_tag="PKY09_04555"
     CDS             complement(9911..10495)
                     /locus_tag="PKY09_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB12159.1"
                     /translation="MQQPVLNDRELVEMYQQGNEDCLTVLIERHQKRIFSAILFLVNN
                     RELAEDIFQDTFVKVINTLKKGSYNEEGKFLPWVLRISHNLVIDHFRRNQRMPMVHDK
                     EDYSIIESLQLMDDNVQDKIVKEQIYKEVRLLVEELPYEQKEVIILRHFADMSFKEIA
                     DATGVSINTSLGRMRYALINLRKVIEKKKLVLAA"
     gene            complement(10745..11164)
                     /locus_tag="PKY09_04560"
     CDS             complement(10745..11164)
                     /locus_tag="PKY09_04560"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNB12160.1"
                     /translation="MMSNITFTMLKPDAVSNGYIGAILDHIIKAGFKIKAMKYTRLTA
                     ERAGEFYAIHRERPFYNDLVKYMSSGPIVAAILEKDNAVEDFRKTIGATDPAKADAGT
                     IRKLYAKSIEANAVHGSDSNENAAIEGSFFFSMTERF"
     gene            11266..12309
                     /locus_tag="PKY09_04565"
     CDS             11266..12309
                     /locus_tag="PKY09_04565"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNB12161.1"
                     /translation="MIPKFEQHSIEQLKNLLSEPKRIVITTHYKPDGDAIGSLLGLYN
                     FLVSKKHKVKAVVPSDYPASISFMKGNQKVLDYEAKPRNVAKAFAEAEIIFCLDFNES
                     KRVEAMEDVMMSNKAFKVVIDHHLYPKIQCDILFSFPKSCATSELIFHFIYALDNKYK
                     MKPEVAECIYTGIMTDTGSFRFSSMSADTHRVLAALLDTGMNHVKIHETISDNFSEER
                     TRFLGYCLKDKMQVIHAYRAAYIALTKNELDQYNHQTGDTEGIVNYPLSISDMMMSAL
                     FTEKDDMIKISFRSKGDFSVRELASKFFSGGGHQNAAGGKFFGSLDEAVEHFIAALAY
                     YKDQLQIQDTVKD"
     gene            12350..14014
                     /locus_tag="PKY09_04570"
     CDS             12350..14014
                     /locus_tag="PKY09_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNB12162.1"
                     /translation="MSQSVQQGHPKGLYVLFVTEMWERFSYYGMRAIFILFMVKALML
                     DKAFASNLYGSYTGLVYLTPLLGGYISDRYWGNRRSIIVGGLLMAIGQFTLFFSGSMI
                     GSTPTGTDATASMLMYLGLGFLIFGNGFFKPNISTMVGQLYPKADKRVDGAFTIFYMG
                     INLGAFFAPLVCGYFGDTNDPADFKYGFLAAGIGMVIGVLTFVLLKNKYIVSPDGSPI
                     GMPKVVTTQAVNTEGEADIPSFNMSKILMWLAIGIGLFFVFKSIVEFDLIGSIIYAGA
                     IAVPGLIITDKSLTHVEKQRVWVIFILCFFVIFFWSAFEQAGASLTFFADEQTDRNLF
                     GWEMPASFFQSFNAIFIVIFAPIFAWLWVRLGNAGKEPGSPLKMAIGLFLLSIGYLVI
                     AFGVKGVEPGVKVSMFWLTTLYLLHTFGELCLSPIGLSMVAKLAPLRFASLLMGTWFL
                     ANSASNKFAGTLSSLYPPGQGEFKAAADKGIDLPSILNGSVTATADQLKVLADAGIPH
                     HFPMFMGMQISSLYDFFMIFVIMSAAASVLLFLIYKWLIKMMHGVQ"
     gene            14118..14348
                     /locus_tag="PKY09_04575"
     CDS             14118..14348
                     /locus_tag="PKY09_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12163.1"
                     /translation="MKKLFTLMAVAAMFAMVSCGPSAEEKAAAEKATQDSIANAEAEM
                     KAAEEAAMAASAAAATPDSAAAAPADSAAAAH"
     gene            complement(14378..14557)
                     /locus_tag="PKY09_04580"
     CDS             complement(14378..14557)
                     /locus_tag="PKY09_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12164.1"
                     /translation="MAKLTLIGVALFYIIMQEIIVGIIAIAATVYLIQKFRSKSNGCD
                     KCSSGPQKSGQKKTT"
     gene            14574..16550
                     /locus_tag="PKY09_04585"
     CDS             14574..16550
                     /locus_tag="PKY09_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HNB12165.1"
                     /translation="MNFDRKHLSDDQLIRLYHELVRPRIIEEKMLVLLRQGKLAKWFS
                     GIGQEAVSVGAAMALHQDEYVLPMHRNLGIFSVRNIPLHRLFSQWMGKPGGFTNGRDR
                     SFHFGTQEYKIIGMISHLGPQCGIADGIALGNILKQNNKATIVFSGDGGASEGDFHEA
                     VNVAAVWDLPVIFIVENNGYGLSTPSSEQYRCKSFADKGIGYGIQGVSVDGNNILEVY
                     NTLNELLSDIRKNPRPIIFEARTFRMRGHEEASGTKYVPQQLFDEWGKKDPIANYENY
                     LLESKIISTAQVESIRTKIKTEIEEHLKISYAESDPEVNTVIELRDMYAPSYDTNITA
                     SVAVTEKRFLDAITDGLRQGMQRHKNLVIMGQDIAEYGGVFKATQGFVEEFGKSRVRN
                     TPITESSILGASLGLSINGYKSIVEMQFADFVTCGFNQIVNNLAKTHYRWGQNADVVV
                     RMPTGAAVGAGPFHSQSTEAWFFHVPGLKILYPAFPSDAKGLLAAAIEDPNPVMFFEH
                     KALYRSVSGLVADDYFTIPIGKASKIREGTDLTIVTYGLGVHWAMEALDENKNIQADL
                     IDLRSLLPWDKETVLESVKRTGKVIIFHEDTLTGGIGGEIAAEISSACFQWLDAPVMR
                     CGSLDTAVPMSNHLEWNFLPRDRFKQMLKQLAAY"
     gene            complement(16559..17182)
                     /locus_tag="PKY09_04590"
     CDS             complement(16559..17182)
                     /locus_tag="PKY09_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12166.1"
                     /translation="MHLRRILILFLCVGVTQLHAQTIINSGFEEDAHSSSQKGWITDC
                     KGTGQIKFSNISPHSGKQCLEIAHQNQATCTFSQSISIKDSEIKNYKIIYSCRAEALN
                     GNTGLNIIIRDASGKKLTESSGFIKNAEAKWKTYSIATSVPEGAQTMSITGILNGTGN
                     VWFDEIKIEELKQHKSSSGLWSTASEFMTIAGKYSIGEAIKWAVEKK"
     gene            17300..19453
                     /locus_tag="PKY09_04595"
     CDS             17300..19453
                     /locus_tag="PKY09_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNB12167.1"
                     /translation="MFRYLLPLIFLISTTSWAQHKKLSMEDAILNARTKLAPANLSQL
                     GWIKNSISYYYVDNRLPDATLFVGDDGIVHDEKIISLSEINDKLKRIKTDTLASFPVV
                     EWKNDKVFGFFIGNKEFEYDKTQYAIRNSRILLLPDSATQKDTDPVTGRIAYTIKNNL
                     FVYDAGKNFTVAEDAYPHIVYGSSVHRNEFGITKGTFWSPKGNKLAFYRMDETMVKDY
                     PLIDWNKKPAEVNLIKYPMSGGTSHQVTVGVFDMINKTVVYLKTGEPKDQYLTNVSWS
                     PDEKYVYIAVLNRDQNHMKLNCYSASTGHFIKTLFEEKDEKYVEPQTPMAFVPGHDDQ
                     FIWQSQRDGYNHLYLYNTDGKLIKQLTKGEFVVTEFKGFDKKSERAYYMCTALSPIDR
                     DLYYVTLSDGKMKRVSGDSGIHTCYYNPENNEVLDVFSNVNTPRKIKLILPNGSDAKT
                     MLTAENPLSDFNLGEMKISTLKSVSGELLYTRTYYPPSMDSSKKYPVIIYVYGGPHLQ
                     LITNSWQGGQGDLWFYYLAQEGFIVYSVDGRGSGNRGKNFEQAIFRNCGVAEMEDQLT
                     GLQYLKSLPYVDSSRIGVDGWSYGGFMTISLMTRFPGLFKVAVAGGPVIDWSMYEIMY
                     TERYMDTPVTNPDGYEKSNLLNYVKNINGKLMLIHDTNDDTVVWQHSLMYLKKAVDLG
                     KQVDYFVYPGHPHNVRGKDRVHLMYKITDYFKQNL"
     gene            19470..21380
                     /locus_tag="PKY09_04600"
     CDS             19470..21380
                     /locus_tag="PKY09_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB12168.1"
                     /translation="MGKKTTYCLLLCFCVVSLHAQNLKRCPQTTSKEAAKKTEAAEKL
                     LKSVKNYEEGVELCNEAIEADSTFAKPYLLMGDAAYRKKDFKLMAKAYKGLMNVCPEA
                     SEKAIYRLATYYFDTKKYEEAIPVYTTFLELSPSDTNAADDAEVKLFRAKAFLHPVDF
                     KPVLVKGLSTPDPEYLPIISADNELCFFTRRFEYQSKNMLTSTSVEKFMLSHNLNGKW
                     DTGKPMDLPFNKQNAGNEGGPAISLDNLHLFFTVNVKGNFDIYTSDLTPKGWSEPRSI
                     GKANDSKFWDSQPSLSPDNRTLYFASFRDSVLQTSDLYVTKKTNGEWNSANPITGPIN
                     TSRNEKAPFIHPDGKTLYFSSDGHPGMGGYDIYYSKKDANGNWGKPVNLGYPINTDAD
                     EVGFFVSTDGTKGYFASNKFGTGGYDIYSFDMPVDARPEKMVLFKGQLKNDDNEVPDS
                     ARIEIRNISSKQVEQIDYDAYSGKYAGVLQANSEYLLTIQKDDYAFTSKLINAAKVDS
                     PAVTVELDLRKIKEGEAYNLNDILFETDSYQITDISKAVIQDFAGFLKGNPKLKVAIY
                     GHTDNSGKAEDNLLLSKNRAKAVYDYLITLGIEATRLSHNGFGQTKPVAPNTTEKGKS
                     LNRRTEFVVLKK"
     gene            21385..21879
                     /gene="idi"
                     /locus_tag="PKY09_04605"
     CDS             21385..21879
                     /gene="idi"
                     /locus_tag="PKY09_04605"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNB12169.1"
                     /translation="MEFVILVDEQDREIGEAEKMQTHKLGLLHRAFSVLIYNTKGEML
                     LQQRAETKYHSGGLWSNACCGHPRPGEKIEDAARRRLKEELGFNCSLKFSFKFIYKAS
                     FENSLKEHELDHVFTGIYDSEVLPDKNEVQAYRWVTMENLKNEIIENHSSFSVWFQKI
                     IAVL"
     gene            22021..22623
                     /locus_tag="PKY09_04610"
     CDS             22021..22623
                     /locus_tag="PKY09_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB12170.1"
                     /translation="MPVLAFAQSNQERLVQLSGVIVTGDSLSPVPYTGIMIKSSSRGT
                     VSDYYGFFSIVAHVGDEIEFSSVGFAKTNFKVPDTLTENRYSLIQILNRDTVLLNQAV
                     IYPWPTREQFKQAFLDLKVPDTDMDHARRNLALETMREESIRIDMDGSMNSKFVYQKQ
                     QSRLYNTGQLPVNNLLNPIAWAKFIQAWKSGEFKSKSKDD"
     gene            22623..25148
                     /locus_tag="PKY09_04615"
     CDS             22623..25148
                     /locus_tag="PKY09_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12171.1"
                     /translation="MRQLVIFLSLFFYCYSLNGQDATARIHGKISGTDRKPLPGVSIG
                     VAGTSLGVISDEHGEYSITVPSNRELSIVFSSIGYEQATVKLILAEGEKKQLNRTMLP
                     SIVPLPNITITDPRERSGTLIKIDPKAVEEIPNPSGNFETILKTIGSGVVSNNELSSE
                     YSVRGGNYDENLVYVNDVEIYRPQLVRSGLQEGLSFINPDLVSGIKFSAGGFDAIYGD
                     KMSSALDIKYKRPTEFAGKITYGLLGGGLSLEGTSRNNRFTYLIGGRQKSTKYLLGTL
                     DTKGNYKPVFYDVQALLTYDVSEKTTISLLGNFSKSRYNIVPQTRTTDFGTVSQALRF
                     TVYFDGKEVDDFTTTTGAIIFSHSPKKNINLKVIGSVFNTREEETFDVLGQYFLDELE
                     KDPASQNFGEVKLNLGVGSFLNHARNFLDGKVYSAEHKGTYNYRHNELLWGIRFQHEQ
                     FSDQIKEWNYIDSSGYSLPHPQDSVGGSLPSQQQLLLQDVISRDNRLSSNRLSGYLQH
                     NFYFSSTSNFILTPGIRFTWWSLNHVLNISPRIALAYKPRWKRDFNFRLSGGFYYQPP
                     FYKELHNPDGSLNTDLKSQQSIHVVGGTDYYFKAWGRDFKFVTEAYYKWLNDLIPYKV
                     DNVRIRYLGKNNSKGYATGVDFRINGQFVNEAESWASLSLLKTEEDIQDDVYYIRLNA
                     RGDTIRGYTIDKQAVDSIQVIPGYLPRLTDQRLTFSIFFSDYLPKFPTFKMHMTLVFG
                     TGFPVGPPGNNRYTDINRYPFYRRVDIGFSKIFIDENRRAKSRFTALNKLKALSLSLE
                     VFNLFQVNNVVSYTWVSDVYGTKYGVPNYLTGRQLSLRLSAKF"
     gene            25291..26010
                     /locus_tag="PKY09_04620"
     CDS             25291..26010
                     /locus_tag="PKY09_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNB12172.1"
                     /translation="MRNHSESKFLTERQSRIGEFLFTLKVVKEFIKGFRTLHFVGPCV
                     AVFGSARFKEDHHYYQLGREVGANVSRLGFTVMTGGGPGVMEAANRGAKEVGGFSVGC
                     NIILPREQQPNKYLDKMVEFHHFFVRKVLMFKYSYAFVVLPGGVGTIDEMFEALTLIQ
                     TKKVGDFPVVLMGKHYWQPVIDQLKVFDEHKTALRSELDSLLITDSLDEMTEYLRVKA
                     IERFKLTKAMKFNRSKWFLED"
     gene            26107..27111
                     /locus_tag="PKY09_04625"
     CDS             26107..27111
                     /locus_tag="PKY09_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNB12173.1"
                     /translation="MKVFRLCFFIFISSMLQAQDIHFSQFYNSPLTLNAANTGLYEGD
                     YRFANTYRSQWSKLDPGYRTNAFSFDKTLYTGADQISGGFLFIYDRSGINRLQSIRLG
                     LSAAYHRTFGVHSLSAGFQWNYIFKSIDRSKITMPEQFNMNTGYFDPSMPTGEVLLDE
                     KRSFGDINAGLVYQVELNKHFIQAGASFFHLNKPGDTFFNSNNKLPVRTCLTVSDKIG
                     IGNDYLLHARLLMMQQVKANEYVAGVTLAKQLSNGESKFTEVNGGLYVRTSPGNGTDA
                     VIPVIGIKYDLFDVALSYDINISSLHTATASRGGFEISLIYTALSSRLFKIKIPCERY
                     "
     gene            27098..29185
                     /locus_tag="PKY09_04630"
     CDS             27098..29185
                     /locus_tag="PKY09_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB12174.1"
                     /translation="MNAIKTKVIVLLALVFSTVHLFAQQNDELKKYGTPQLKRMGSYA
                     AKINDFSSSAMYYEEYCKRKPTNEKVWFLYAESLRKSKDYRKAEDAYMKAYNLNPKKN
                     TLALYHYANVQKSLGNYKKAEEYFTKFKKEYTGTDKSVYTKLIKNSGDAAKFADSLMH
                     SPSRVAIEHLPDDINTSHVESSPFYINDSTIIYGSLKTDQVTFDFNPRDSSADEPFRK
                     IYVAVRDSEIWKDDGELPGFYNSNSAHTLNGVYSQDKSRFYFTRCKRNRQNKVICAIY
                     VSFLNNGTWTEPVSLGTEVNDPKYTSTQPAIGLNTQKKGEEIIYFVSDREGGKGGYDI
                     WYTSFDNRNKSYRTPINLGTKINTSGDEVTPYVDAQTQTFYFSSDGWQGLGGLDIFKS
                     IGGMKKWTHPVNLGYPVNSNYDDLYYVLSPSNKDIGMIVSNRNVTDNGVNKGPCCDDI
                     FEFNILDAVRLNVKGFLYETDSTQKGKKLAMMPVRRARITLEMKNAEDSTFIPVSTSN
                     TDETGHFNLIVSPRQEYRITARKEGYLYESVALSTMNKTRSGDISIDIHIRSIPETPL
                     LLTNIYYEFASPLLTAKAMAALDTTLVIFMQRNVDLGVEIRSHTDAIGSDDANMTLSQ
                     KRAESVVNYLVAKGINANRLRAKGFGETMPVAPNTKPDGRDNPKGRQLNRRTEFKILG
                     KMRDGVIREETDF"
     gene            complement(29188..29772)
                     /locus_tag="PKY09_04635"
     CDS             complement(29188..29772)
                     /locus_tag="PKY09_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HNB12175.1"
                     /translation="MIYEFNGFRPVVDSSSFVHEQATVTGDVIIGKQVYIAPGAAIRG
                     DWGTIIIEDGCNIQENCTIHMFPGVTVNLKQGAHIGHGAIIHGATIGKNSLIGMNAVI
                     MDNAVIGDGCIVGALCFVPDGMIVPNRKIVVGNPAKIIKDVSDEMLHWKTKGTELYQQ
                     LPHELRATLKPCAPLRSIEPNRKKHDAVYTTWKK"
     gene            29906..34339
                     /locus_tag="PKY09_04640"
     CDS             29906..34339
                     /locus_tag="PKY09_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12176.1"
                     /translation="MSKYFVAILLTFTCLASSVRSQTLKSFNTDPSSYLLDLKSFFEE
                     TNKKEAEKIMEEFKPVFLSKFDVQQQQSIITTSNLMLKKRMKAFPDFVTYTSALTAFA
                     SSGQEATTFTSWHATFAKAIAKLSVRKLGDFLEISQLLFRNNTLYESSAVTWSASNNK
                     FAFGFDSLPKVTFSGMTLRCFGKGDSSVIEGTKGVYYPNNLLFFGDGGTVNFTRAGIS
                     VSEANAIVKRYAINLKGSEYSMDSVAFTYKKYFDQELKGRYIDKLLANVNDSNATYPR
                     FYSYAANLDIKNMVKDADYKGGFSLQGSKMVGSGNRRQDASITFNLNGKPQLKLLSQG
                     FIFRPDRIVSVNAAAVIYWEKDSIYHPGVEFKYIYGDKTVTLTKNGQTAINSPYFDSY
                     HKMDLDFDQLVWKITDPLMDLKMISGGGESKLKFESVNFFSRQRFDKIQGLSEVHPLF
                     KIKQFAEEHNAKVISVPEFAEYMKLTENTVRNQILQLSSLGFISYDADADKFIVKDKV
                     MYYLNARTGKVDYDELKIESMISGLSNATINLLNFDINMRGVSHVLISDSQEVYIQPL
                     DQEMTLKKNRDIVFNGRVHAGRLDFYGKEFTFNYANFNIDLKNVDSLRFKVPSDTVDE
                     RGRKRLIPIKSILQQLNGKLSIDNPTNKSGFVPNEEYPVFVCTNKSYIYYDYPEIFGG
                     VYKRDKFYYHLDPFSIDSLNTLTKEGLVFEGSMVSADIFPEWRQQLTVQRDYSLGFVT
                     NTPDAGFAAYSGKGKYRQQISLSHEGFLGKGEVDYLSSTSKSNNIIFFPDSMHAVTQS
                     FAIRKEQVGATRFPDVKNEDVFVNWRPKSDKMFVFKRSKDFALFNGEAVLDGNLVLMP
                     SGIKANGIIRYQQSQLISSNHFIREHSYGSDSAEFKLNTDAGDLLALHTQNVKANIDL
                     DKRIGDFSSQGEKAKVEFPVNEYISYIRDFRWFIDKSELEFGGSDAIADKANEFVSIN
                     RQQDSLRWDAPLATYNLKSYLLKGFKVKEILVADASIVPDLGNVVVERNAVMRPLVNS
                     KVIANTTSRYHTMLNTNITIQSRMNYSGDGNYEYMDQTKVKHKIRLTQIGVDTSKQTF
                     AIGEIPDSSRFFISPTIQYKGKLRINAPKENLYFNGFAIVNHHCNTFERNWFGFAGDI
                     DPKGVNIPIQSPANESLQKLYSGIYFGSDSTMMYPAFLSMKKHSSDAEVISAEGYLTF
                     DNNLQQYKISGTIPGTDSTKSASVITNTVILDDKRCQLIGEGRINLTQDFGQLSITNF
                     GMVNYSTVNDSIGFDAVMAADFMFNDDALKQMAEMFNSIPALQPFMDDRPVYRNALTN
                     MIGKNKAEKLLNETGLYGSARRIPSELQHTLVFSELKFYYNKERASYISVGNIGLGVI
                     GKTYVGRMVKGKVEIMRRRSGDVLNIYLEALPDIWYYFNYQRGVLQAISSDEKFNKPI
                     EEMKPEKRVASEKGGLEPYQFMLATERKKNEFLKRIKDADL"
     gene            34386..34862
                     /locus_tag="PKY09_04645"
     CDS             34386..34862
                     /locus_tag="PKY09_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013668352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB12177.1"
                     /translation="MQREHHYKANIVWTGNTGYGTKDYLSYDRSYLVRIENKPDFHCS
                     SDPAFRGDKTKYNPEELLLASLSSCHMLWYLHLCSDAGVVVKSYSDNATGIMVETSSR
                     AGKFESVTLNPFVLIADDSKKDFAISLHNRANEMCFIANSMNFKVYHRPTITIETG"
     gene            35011..35979
                     /locus_tag="PKY09_04650"
     CDS             35011..35979
                     /locus_tag="PKY09_04650"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNB12178.1"
                     /translation="MKNTFFTMAFILCAFFVKAQSSNVILFTENGEKFYAILNGVRQN
                     TSPETNVKVTGLNANFYKLKVIFEDTKLGEKDFNLAFNPGMETTFSIKFNSKKNSYSL
                     RPLSEVPLAQALPPAPNQTIVVYSEVPAPPPAATTVTHTTTTTTTDGTPMPGDNVNVN
                     IGLNMNETGGGINMNVSGGTTQTTTHTTTTTVTHTTTSEPPAAPPVAPTPAPVVYLPG
                     YNGPYGCPVPMAPQDFSDLKQSISSKSFEDSKMTIAKQVLRNNCLFTSQVKELMGLFT
                     FEESKLDFAKYAYGFTYDIGNYFKVNDMFTFESSIDDLNNYINSRH"
BASE COUNT        11277 a         6156 c         7018 g        11718 t
ORIGIN      
        1 atgctcatca tctatccaca aaatattata aaatatatcc tccataatta gttaaccagt
       61 ttgattggaa attggaattt gaacttcacc aaaaagaaag ggtcttcgtt caatgatagt
      121 atccaatccg ggttattaaa atcacttgca attctgtgta tgtcataacc gcctaaacca
      181 ctgccattgc ttgaatcggc tgtcgagtat ttggatataa acttctctct gtcgaagttc
      241 ttgggaaatg gtttaccgtt atttctgatt tccattgcaa gggaatcatc aacttcagtc
      301 agttcaataa ctacatcgtt acctgtcgct tttttatcaa atgcatactt gttagcattt
      361 gtcagaatgt tatccatcaa tgttttgaaa agcgttttgt tggcataaat gcctctttct
      421 ttcagctttt caccttttag caacaacttc tttattttga atttaaatcc gttattagac
      481 aggtcattaa taatactgtt aatgtcagaa aggggaacta ctttcttttc gtacttagat
      541 aggacaagtc cgttttcacc cttttcaagt acatctgtaa tgaagtttac atcacgcttg
      601 atttctttta gagccgatat aatatcaata tcatagaatt cggaaaaggc tttattcaat
      661 gcctcaaaac cttccttttt cttggttaaa aattccaaca agttgtcaga ccaatcaaga
      721 atattttgtc ggggtcttcc taaagtatgc ttcaatgaag caaattcatt gaattgcttt
      781 ctattcttac catgcgccaa agcgttgcgt tcttcctgaa gccttttaat cttgtcagat
      841 aactcgtaaa tcccttgaat ctttgcccgt tgctcttcta ttgaaggaag ttttataaca
      901 atctccatca aatcttcttt tcgaatgatt ggtattgttg ctcctaagcg gtatgattca
      961 agttgctctt gtacataatc agcatgtagt tcgtagatca aataagcttt atcagcaatt
     1021 gcttcattta ccttaaagga aagaatgtcc tgacttctaa aaataggctc acctttgaac
     1081 tcaaaaagtg ttggcatcaa tgtacgccaa cgcatagcaa gcagtaggca ggattccgag
     1141 acaagattaa tatcaggtcg cctcagttct gtttcttcaa cgctggaaac atcaagggta
     1201 aaatcaactt tatcgtcttt taaatcacga atacgaatta gtttacctgt ttcaggcagg
     1261 ttgcctcttt ggcctctaac taactctaaa atatcgccta actttacacc gtcaatttgc
     1321 ttttggaaat aacgtgcaac actcaggttg taatcgttgt cacgaatttg ttcattgtaa
     1381 actatcttaa caacattatt atcttcttta ttgttatgaa tgtaactatt taggctgtaa
     1441 tcattcagta ccttttctct aggtcctttg gcaatgacaa aattcttagc atcaacaaat
     1501 tttaccctac cgggtaactt cttgtttcta tcaagaacta aaatgattaa tggtattcct
     1561 gtatttaata acaaaccacc gggcaatgaa ataatggtgt caattaaatc ttcttcaacc
     1621 agatgtttcc gcaaacgctg ctcatacatt cctctgatga aaaggaagcc ttgtggtaaa
     1681 agggcgatta acttgccgtt ttgctttaag gactgaacac ccttttcaat caaaaactgt
     1741 tcaatggttc tgatattagg ttcaatatcc ctgtaatgac gcccaagtct catcccgaat
     1801 gggggattgg aaagaattag gtcaaatttt tctgattcgt caggccaatt taaaatcgaa
     1861 tcatcacaaa catattttga attgcctggt ctctcgtatg ccatgagtct taaagcaccc
     1921 aaagcccaag ttttttgatt tagttcttgt ccaaaatagt cctgaccttg gtcaagatta
     1981 acgccaaagg atgctaagcc cgcaaaagga ttaaaaactt tggcatttct tttcaaatca
     2041 gccaagccac acataaaacg ggttaattca acgggttgaa taaattcacc tgcatatctt
     2101 ccttgagatt gcgaaattcg atacaatacg ctgtcaaaaa catcagggaa attttcagtt
     2161 agaacttgct tgttaatctc agcaagaact ctgaaaaagt ttctaatacc attattactt
     2221 aatttttgaa ttgatggctt gaagccttga aagatgggag catattgatt gtctaactca
     2281 ctattagaat tacgtagtcg ttcaattaac ctttctttta agtcatgatt ttcattaact
     2341 aaatcaattg atataagatc gtctttatat gcagaaagta gaaacaatac cacatgataa
     2401 tcatccgaag aaatgttttc actcctcaga acatcaaaca ttatccaaat tttggatttc
     2461 actccttcaa gtggcaatat ctcttctatc ttaaattcct tcatcatacg ctattttctg
     2521 attaacgaaa caaaggtgag atttatctgt gcagtacaag tgcatagttt aaatatattt
     2581 tttataaacc gtttcagtaa gtcttttaaa tctctctcta agactatctg gttcaataac
     2641 ttctatagca tccatgtagg aaaataagag tgaaattaac tcgtgattta tctggacaac
     2701 cagttcaatc aggacttctg tttcagttct gctttttatt ttttgtgagc cgtgcagtgg
     2761 tttactctca atatatggcc aaatttcttt actaagcttg ataagtaact tttctattgg
     2821 agcatctctc ttaacagtta cgccaacaac atcatcaaaa tattcatcga agtttacttt
     2881 ttcatttttt tgaaactttg tttttgattc ttcaaattcg attattctat caagagcaaa
     2941 attggtaatt gacttataat cttcatttag cccgaaaaga aaccaacgat tgttatattg
     3001 tttcaaatac catgggtgca aaattatttg tgttggagtt tcttgcctat aaccttgata
     3061 tgatattttt aatgcctttt cgtattgtat agcattgaaa attccagtga aatgacttaa
     3121 ccctttcaga aacgggtttt gttcaaatcc gactgatgac tgattgttgc cttctaattt
     3181 aaatgtatct tcaagtctga tttgcatttc ttcaatccat tcaaattgtg gcaatccctt
     3241 aaatcgtgtt aaaattgtca aggtctcttt tagttgttcc gcctcagttt gattaatccc
     3301 ttggcttttg atggagaaag atttgtctga atatctatag taaactcttt ttccctcttt
     3361 aaatcgttga agtggaattg accaaccttg ttcactctcc ataaaggtta tgtcgtcaaa
     3421 aacctgtctt ctttttactc catcctcaac tcccgcaaat tcatagatgg cttcgttaca
     3481 ggcaataatt aagtcttcta taaaaaattt cctcccatag ttactaaaac acctgtcgag
     3541 cgcatggtat cgaatggtag catgtttatt tgtcgccata ctaaaagatt gaagttgaaa
     3601 gtgggttggc aaaaaacggc tcttttggtt ttgctgaagg gtcggctttt tctttatttt
     3661 ctaccacttg tccaaatagc aaaacacggt cagtttgaag gtctgttcgc ttagtcaata
     3721 tggtttgtat atcgtttcca ttatcagtca ttatcttgct gtgtcgtctg tctttttagc
     3781 attggtgtta acgtctgtct atgaaaaaaa tatttaaaag catttacaaa tataatttaa
     3841 ttactttagt cttaggaata aaaacagcaa ctaaaaatgc ctataagaaa aggaaacaac
     3901 tgcgaacaaa ttgctttttt aaactaaaag agtcagatat tcaaagataa agaaatccgt
     3961 tttatagatg cttttgttga tgggttagat ttaaagtagt gcggtattca atcgctgata
     4021 taaaacgaaa aaaaagctgc cttcagatat gacgacagct tttaaattca caaattttaa
     4081 tgctatttat atattggggt tattcccatg ttgtagaaca tgaatgacca gatgtcagcc
     4141 tgctcttcaa taatttttga agtgggtttg cctgcaccat gtcctgcttt tacatcaatg
     4201 cgtattactt gtggtctgtt gcctttgtaa cgctcttgca gggtggcaat aaatttaaat
     4261 gagtgtgcag gcacaactct gtcgtcatgg tcggcagttg ttaccatagt tgccggatat
     4321 tccacacctt ctttgatgtt gtgtaatggt gagtaggcat acaggttttt gaaattttct
     4381 aaactgtcgc tgctgccgta ttcaacagcc catccccagc caacagtaaa tttatgatag
     4441 cgcaacatgt ccattacacc aacagcagga aatgctacac gatataagtc ggggcgttgc
     4501 agcatgcatg cacccaccaa taggcctccg tttgaacctc cctgtatggc taatttagat
     4561 gatgaggtgt atttttcttt gatcaggtat tctgctgctg caataaaatc gtcaaataca
     4621 ttttgctttt tgagaagttt gccttcattg tgccattttt ctccatactc tccaccacca
     4681 cggatgtttg ccattacgaa gatgccgcca ttttcaagaa gtattaatct tgatgtgctg
     4741 aaagatgggg taagactgat gttgaatcct ccataaccat agagtagggt aggattgttg
     4801 ccatcgagtt tgattccttt tttataaact aaaaacatgg gaacttttgt tccgtccttg
     4861 ctgttgtaaa atacttgttt tgtttcgtac tcttcaggat tgaatttaac atcggatttt
     4921 tgccagatgt ctgacttgcc tgatgcaata tcgtacttat atattgtagg aggataggtg
     4981 aatgatgtaa aggtgtaaaa aattaatgca tcttctttgt cgccactaaa tccgaaagct
     5041 gtacctaatg caggtaatgc aatttcatgt tcgagattac cctcaaggct atattgataa
     5101 acatgtgttg ttacatcttt aaggtaggaa acaaaaagtt taccacctcc ggtagctgca
     5161 ctttcaagta gctcgggttt ttccggaatc acatctttcc agttttcctt tttaggattt
     5221 tttggatcaa ctaaaattac tctttgatta ggagcacctg catctgtttg tacaagtaac
     5281 ttgtcgccaa tattgtttat tacgctgtag ttgtcttgaa atccggcaaa caaaagacca
     5341 aaatcctttt gattgctttg caaatctttg taatacattt ctgttccgtc agtaccttca
     5401 gaaacgtaaa tgataagaaa gcgctcgtct tctgttacag aggcaccata gtagcgcaag
     5461 ggtttgtctt tgttttcata caccaaaaca tctttatcct gagtgtcgcc aaggcggtgg
     5521 taatagattt tatggtattc gtttttttgt gaaaattctt taccttttgg aggggcatca
     5581 taacgtgcat agtagaagcc attgtttttc catgatgcac cactgaattt tacccattcc
     5641 aatttgtcgc tcattagttt ttttgtggca acttccatta cataaatagt ttgccagtcg
     5701 ctgcctgatt gattaatagc ataggcaaca tatttcttgt ctttagaaaa ccctaacagt
     5761 gaaactgctg cagtaccatc gctgctcatg gtgttggggt cgaggaatac ttccggtttt
     5821 ccatcaagac ctttctgata ataaatgaca ctttgatttt gcagaccatc atttttgctg
     5881 aaaaaataat attcaccggc acgaaaagga ttggaatatt ttgggtaatt aaaaatatcg
     5941 gtaagtcttt cttttatttt atttctgaat ggaatttttt caagatagcc aaaggttacc
     6001 ttattctctt ctgtaaccca tttttttaca tcttcggcag tgtcgttttc taaccaacga
     6061 tacgggtcag aaacttttac accaaaataa tcatcaacct gatctgattt tgcagttatg
     6121 ggataggtta atttttctga tgtggccggt tcagatgccg gtttgcatga aatcataatt
     6181 gaagtgattg ttaatacact cagtaatggt ttaatcattt taatttaata ttgtgtttga
     6241 atgtgcaaaa ttaaagctat acattctttt tagtctaaga taaaacttaa ttattggtta
     6301 aagtatatgt ttgtttataa tgtaactgta aatattgata atgatgttgc ggcagaatgg
     6361 ctgcagtgga tgcaggaaat tcatattcct gatgtgttaa ataccggatg ttttattgaa
     6421 aatcgtattt tcagagtgat ggctgatgaa gatagcggtg gaaaaactta ttctgttcaa
     6481 tattatttca ggacgatgga agacatggaa cgctatcaga ataattatgc agcagaactt
     6541 cagcaggaac atactgcacg atataaagaa aagtttgtgg cttttcgcac gctgcttgaa
     6601 gtggtgtgtt gaagtaagtt gtgaaaggtt aatgttgcag gtgtaatatt ttgacaactt
     6661 tcagttgaag tattgagtat gagcttttta ctttgctcta aaaacaaaaa ctgccatgaa
     6721 aattatacat ggcagtttaa tttctttgaa tcaaaatatt atgaatggct gtgctcagta
     6781 tgaactgatt tgcttccgta tgcggggcat ttttcacggc ttttgcatga attcaatgaa
     6841 acaattagag ataaaaaggc taatgcaaca agcaattttt tcattgtaat gagatttata
     6901 gtcggcaaag aaagaaaaat caagccaaag tcagtatatg attcaccaat gatgtgaaaa
     6961 tacttattaa caatcggcta ccataaaacg caagtactta aaatttattt caagagtaaa
     7021 tgtatggttc tgtgtagtta ttttagcaga caattaacag tatggagtca agtcagataa
     7081 aaattgaaga aagctggaga aagatgcttc aggagcagtt taacatgcct tattttgaag
     7141 aattgaagaa attcgtcaaa caggaagctg ctctttataa agtttttcct cccggacctc
     7201 aaattttcag tgcttttgat tataccccct ttgaaaaagc aagagtggtg ataattggtc
     7261 aggatccgta tcatggagca ggtcaggcca atgggctttg cttttctgtt tcagaaggaa
     7321 taaagccgcc accttcattg gtaaatattt ttaaagaatt gaaaagtgat ttaaatattc
     7381 ctattcctgt tcacggcaac cttgagaagt gggcgcgaca gggtgtgttt ttattgaatg
     7441 ccacattaac agttcggcag agtatggcag gttcgcatca gggaaaaggt tgggagcagt
     7501 ttacagatgc tgtgattaga actttgtctg aacataagtc aggattggtt tttttacttt
     7561 gggggcgttt tgcacaagca aaggaaattt tgattgataa taagaaacat catattctaa
     7621 aatctgcgca tccttctcct ttttctgcca caaaattttt tggctgtaag catttttcag
     7681 caacaaacag aattttaaag tcagaaggat tgcaacccat tgattggaat attaattaat
     7741 gctcatgcat ctgctacgac accttttact ttttttctca atctgctttt tttatgtgca
     7801 actgtcattt gcacaatata gacttgaact taaaggtgat gttgtttcaa taaaatataa
     7861 aaaggaattt gcagattctg tgttgttgaa aaaagaaata accaaaatta ttaacgaagc
     7921 tcagaaggaa ggctttattg cagcgtctgc agattctttg gctaaggacg gcaatatcta
     7981 tagtgtttat ctgcataaag gcaatcgcta taagtgggcg cgaataacag gtggcaatat
     8041 tgatgaacgc gtattgaatg aaactggcaa tagtgaaaag aaatggcgcg gcagcaaggt
     8101 cagtccgcca aggatggcac aaatgtttaa gtcactttta cattggtacg agaataacgg
     8161 ctaccccttt gccgaagtga aattagatag tctgaatttt aatgagcagc aaatttcagg
     8221 agttttaaga gtcaacaaaa gcgaacttgt tcgtgttgac agcatcatca ttcgtggtga
     8281 ttctaaatta agcagaacat ttttatttaa ttatctgcgc gtgagtcaag gggatgttta
     8341 taatgaaagc atcattcgta atatttcaat ccgattgaag gagttgccaa tggtttcaga
     8401 atatgcaccg ttggttgttg catttgaagg tggcggtgca gtgcttactt tgtttctgaa
     8461 caataaaaaa gcttcgcagg ccgatggtat tattggtgtg ttgcctgatg caacaaagag
     8521 tgggaaagtg aatatcagtg gagaactgcg actgaaattg ctgagtgcct ttggtcgtgg
     8581 cgagcagttt gagattaact ggaaacagcc attgcctaaa acgcaggatt taaaaacatc
     8641 attaagttat ccatttctat tctcacttcc atttggcctt gaaggcggat tgaatattta
     8701 caaacgtgat acttcttatg ttgatgtatt gttaaaagga ggtattcaat acctgatgaa
     8761 agggaatgac agaataacgt ttgttgtttc caataaaaaa tcttcactgc tttcaacatc
     8821 ttcttacaaa aatgcaaccg ttcttccagc ctttgcagat gcaagtatta caacttacgg
     8881 agtagtgtta aagaaagaaa aagttgacta cagactaaac ccgcagcgag ggttcatggc
     8941 tgaaatttcg ggcggtgcag gagttaagaa gataataaaa aacagcagca tcaatagcga
     9001 attgtataag ggtataaagc tgaatagcga tatttataaa tttgaaggtg cagcagattt
     9061 ttatcagaca ttgttgcctc gtatagtttt gaatactgga attgcttttg catggatgca
     9121 tgccgatgac atttttcaga atgatcaatt ccgttttggc ggactaaaat ctttaagagg
     9181 atttgatgaa gatgtactct atgcaacatc atattattta ggaaagatag agttgcgtta
     9241 tatcctcgac agaaatgcct atttgcagtt gttttataat caggcctatt atgcctcaga
     9301 aaccagagca gggaatatgt atgatagtcc atttggtatg ggagcaggaa ttacttttga
     9361 aaccaaactt ggaattttcg cacttaatta tgcaattggc agccagcaaa acagtgccat
     9421 tcagttccgg caggcaaaag tccacttcgg aattgtaaat tacttttagt cagacaactc
     9481 agatatatta tcacagaaga aattattatt ctgaagatgg tattgccatt tgtttacatt
     9541 aacttaatat tgggttaata gtgcttaagc gctttgtaat agcaacctca aaatcagaat
     9601 aacaaaaatc ttgatgtcag aattacttta atgaatgtga gtagttcaga atgtttttaa
     9661 taactgatga tgatggattc accatgcact ggtcaatatg atccattacc tgaacaattt
     9721 cctgatagct ttcttcagtt tcaggattgc aatcaatttc atgctgaatc aacacagtgt
     9781 cggataactc gacttcgtta aagtagtaaa gaattaaatc gtgtgtagta gaactttcgt
     9841 acataggcgt gttacttatt tacacctata acgcagcgtt gtcaggaata ttttatatgt
     9901 cctgaaaatt tcaggctgcc agcactaact ttttcttctc aatcactttt ctgaggttga
     9961 taagggcata gcgcatcctg cctaatgaag tgttgatact cacaccggta gcatcggcaa
    10021 tttctttaaa actcatatct gcaaagtgtc tcagaatgat aacttctttt tgttcgtatg
    10081 gaagttcttc aaccagcagg cgcacttctt tataaatctg ctcctttaca attttgtcct
    10141 gcacattgtc atccatcagt tgcagtgatt cgatgataga ataatcttcc ttgtcatgaa
    10201 ccattggcat acgctgattg cgtctgaaat ggtcaataac taagttgtgg ctgatgcgaa
    10261 gaacccaagg taaaaattta ccttcttcgt tgtagcttcc tttttttaag gtgttgatta
    10321 ccttaacaaa agtgtcctgg aaaatatctt cggctaattc tctattattg acaagaaaga
    10381 gaatggcaga aaatattctt ttctgatgtc gttcaatcag aacggttaag cagtcttcgt
    10441 taccttgttg gtacatttcg accagttcgc ggtcgtttaa aacaggctgt tgcatagtga
    10501 actctttttt ggaaataagt gataaagtag agttttatgc tatagttttc ctcctgtttg
    10561 aaatgtgatt atttgagatg ttttgagata tagaacgtgt tatttttaat aatgttgtat
    10621 agctcaaaaa atatttttat taattattca gtaaacataa agaaaaaatc ctgccatttc
    10681 aactttcaag taataagtga caggattttt cagtaaaatt tcactttcga aggaatatcg
    10741 gtgcttaaaa tctttcagtc atgctaaaga aaaatgagcc ttcaatagca gcattttcat
    10801 tactgtcgga accatgaacg gcattggctt caatactttt agcataaagc ttacgaatag
    10861 tgccggcatc agcctttgca ggatcggttg caccaatagt ttttctgaaa tcttcaacgg
    10921 cattgtcttt ttcaagaatt gccgcaacta taggtccgga tgacatatac tttaccaaat
    10981 cattataaaa tggtctttca cggtgaatgg catagaattc acctgcacgt tctgcggtta
    11041 aacgtgtgta cttcattgct ttaatcttaa atccggcctt aataatatga tctaaaatgg
    11101 ccccaatgta tccattgctt actgcatcag gctttagcat tgtaaaagtg atgttactca
    11161 taattaatat ttgtattttt ttgtggccgc aaaagtagtt gatttcagct ttggtttgac
    11221 tgatgcaaat agcttacatt tgtaaaaatc gcttttagaa agtttatgat accaaaattt
    11281 gagcaacaca gcatagagca attaaagaat ctgttatcgg aaccaaaaag aatcgttatc
    11341 accactcact ataaaccgga tggcgatgct ataggctcac ttcttggatt gtataatttc
    11401 ttagtctcca aaaaacacaa agttaaagct gttgtgccaa gtgattatcc tgcatcaatc
    11461 agttttatga aaggcaatca gaaagtgttg gactatgaag ccaagccacg taatgttgct
    11521 aaagcttttg ccgaagcaga aataattttt tgtcttgatt ttaatgagtc aaaaagagtt
    11581 gaggctatgg aagatgtaat gatgtcaaac aaagctttta aagtagttat tgaccatcat
    11641 ttatatccaa aaatacaatg cgatatttta ttttcttttc ctaaatcgtg tgcaacaagt
    11701 gagttgattt tccactttat ttatgcatta gacaataagt acaaaatgaa gccggaggtg
    11761 gccgagtgta tctatacagg aattatgact gacaccggtt ctttcaggtt ttcgtcaatg
    11821 agtgctgata cacatcgcgt tttagcagcc ttactcgata caggaatgaa tcatgtgaaa
    11881 atacatgaaa ccatttctga caatttctca gaagagcgaa cacgttttct cggttactgc
    11941 ctgaaagata aaatgcaggt aatacatgct tacagggctg cctatattgc gctaaccaag
    12001 aacgaactcg atcagtacaa tcatcaaacc ggagatacag aaggaattgt caattatcca
    12061 cttagtatca gtgacatgat gatgtcagca ctatttacag agaaagatga tatgattaaa
    12121 atatctttcc gctcgaaagg tgatttttct gtacgtgaac tggcatcaaa gtttttctcc
    12181 ggtggtggac atcagaatgc tgcaggaggc aagttttttg gaagtttaga cgaagcagtt
    12241 gagcatttta tagctgcatt agcctattat aaagaccagt tgcaaattca ggataccgta
    12301 aaagattaat ttttttacat tagccgcgct ttaaatttaa atcaaacgta tgagccaaag
    12361 tgtacaacaa ggacatccca agggactgta tgtgttattt gtaaccgaaa tgtgggagcg
    12421 tttcagctat tatggaatgc gcgccatctt tattcttttc atggttaaag cattgatgct
    12481 tgacaaagca tttgcttcaa acctttatgg tagttatacc ggattagttt atctgacacc
    12541 gttgttaggc ggatacattt cagatcgtta ttggggtaac aggcgttcga ttatcgtagg
    12601 cggcttgttg atggctattg gtcagtttac tttgtttttt agtggctcta tgattggttc
    12661 tacacctaca ggcaccgatg ccacagcaag tatgctgatg tatttaggtc ttgggttttt
    12721 gatttttgga aacggatttt ttaaaccgaa tatttctaca atggttggtc aactttatcc
    12781 aaaagctgat aaaagagtag atggtgcatt tactattttt tatatgggaa ttaatctcgg
    12841 tgcatttttt gctccattgg tttgtggata ttttggcgat accaacgatc ctgctgactt
    12901 taaatatgga tttcttgctg ccggcatagg aatggttatt ggtgtactca cctttgtgtt
    12961 gctcaagaat aaatatatag tgagcccaga cggttcaccg ataggaatgc ctaaagttgt
    13021 taccacacaa gccgttaata cagaaggaga agcagatatc ccatcgttca atatgtctaa
    13081 gattctcatg tggctggcga ttggaatcgg attgtttttt gtttttaaat ctatagttga
    13141 attcgattta ataggctcaa ttatttatgc aggtgctatt gccgttccag gtcttattat
    13201 tacagataag tctttgactc atgttgagaa acaacgcgtc tgggtaattt ttatactttg
    13261 cttctttgta attttcttct ggagtgcatt cgagcaagcc ggagcctcgc taactttctt
    13321 tgctgacgaa caaacagata gaaatttgtt tggttgggaa atgccggcaa gcttttttca
    13381 atcattcaac gcaatattta ttgtgatttt tgcaccgatt tttgcttggt tatgggtgcg
    13441 attaggcaat gcaggaaaag aacccggttc accattaaaa atggcaattg ggcttttctt
    13501 actctcaatt ggctatttag taattgcttt tggtgtaaaa ggagtagagc ccggagtaaa
    13561 agtttctatg ttttggctaa caactttata cctgctgcac acatttggtg agctttgttt
    13621 gtctccaatt ggactttcaa tggttgcaaa attagcacct ctgcgttttg cctccttgtt
    13681 aatgggaaca tggttcttag caaactcggc ttctaataaa tttgcaggaa cattaagttc
    13741 tttatatcct ccgggacaag gtgaatttaa agctgctgcc gacaaaggaa tagatcttcc
    13801 atctatcctt aatggttctg ttactgctac agccgatcag ttgaaagtgc ttgccgatgc
    13861 aggtattcca catcattttc caatgtttat gggtatgcag atttcaagtc tctacgattt
    13921 tttcatgata ttcgtaatta tgagtgccgc tgcatccgta ttgttattct tgatttacaa
    13981 atggcttatt aaaatgatgc atggtgtgca ataacaatta attgttaaca acttagttga
    14041 atagttaata aaaaacacct tcatcattta ttaatcagca tacttttgtc ccgtcaaatc
    14101 aaacaataaa aagaaaaatg aaaaaattat tcacattaat ggctgttgca gcaatgtttg
    14161 caatggtatc atgcggtcca agcgctgaag aaaaagctgc tgcggaaaaa gcaactcagg
    14221 attcaattgc taatgcagaa gccgaaatga aggctgctga agaagctgca atggctgcat
    14281 ctgctgctgc tgctacacct gattcagctg ctgctgctcc tgctgattca gctgctgctg
    14341 ctcattaatt ttaaaaaatt aagaaaaaga accaccgtca ggtggttttt ttttgccctg
    14401 acttttgtgg cccggaactg catttatcac aaccatttga tttacttctg aatttttgaa
    14461 tcaaataaac tgttgcagca atggctatga tgccgacaat aatttcttgc ataataatat
    14521 aaaacaaagc tactccaatt aaagttaact ttgccacact tttttattta acaatgaact
    14581 tcgacagaaa acatttgagt gacgaccaat taatcagact ttatcatgag ttggtgcgtc
    14641 cgcgtataat tgaagagaag atgcttgtct tactgcgtca gggaaaactg gcaaagtggt
    14701 tttccggaat cggtcaggaa gcagtttctg tgggtgcggc catggcactt catcaggatg
    14761 aatatgtatt acctatgcac aggaaccttg gtatttttag tgtgcgcaat attcccctac
    14821 accgtttgtt ttcacagtgg atgggcaagc caggtggatt taccaatgga cgcgatcgtt
    14881 cctttcactt cggaacacag gagtataaaa taataggaat gatatcacat ctcggcccgc
    14941 aatgtggtat tgcagatgga attgctttag gaaatattct taaacaaaat aataaagcaa
    15001 ccattgtttt ttcaggtgat ggcggtgcaa gcgaaggcga ttttcacgag gcagtaaatg
    15061 tggcagctgt atgggattta cctgtaattt ttatagttga aaacaatggt tacggattgt
    15121 caacaccttc atcagaacaa tacagatgta aaagttttgc tgataaagga attggttatg
    15181 gcattcaggg tgtaagtgtt gatggtaaca atattctgga agtttataat accttgaatg
    15241 aattattgag tgacatcaga aaaaatcctc gcccaataat ttttgaagct cgcaccttta
    15301 gaatgcgcgg acatgaagaa gcttcaggaa caaaatatgt tcctcagcaa ttgtttgatg
    15361 aatggggaaa gaaagatccg atagcgaatt acgaaaacta tcttttagaa agtaaaatta
    15421 tttcaactgc tcaagttgaa agtattcgta caaaaatcaa aacagaaatt gaagaacatc
    15481 ttaagataag ttatgctgag tcagaccctg aagttaatac tgtaattgag ttgcgcgata
    15541 tgtatgcgcc ttcttatgac acaaacataa ctgcgtcagt agcagtaact gaaaaaagat
    15601 ttttagatgc tatcactgac ggacttcgtc aaggcatgca gcgacataag aacttggtta
    15661 taatgggcca ggacattgct gagtatggcg gagtttttaa agcaactcaa ggctttgttg
    15721 aagaatttgg gaaatcacgt gttagaaata cgcccattac agaatcatca attcttggtg
    15781 caagtttggg tttatccata aacggctata aatctattgt tgaaatgcag tttgccgact
    15841 tcgttacgtg cggcttcaat cagattgtaa ataatctggc aaaaacacat tatcgctggg
    15901 gacaaaatgc agatgttgtg gtgcgcatgc ccacaggtgc tgccgttggc gcaggaccat
    15961 ttcactcaca gtcaacagaa gcatggttct ttcatgtacc gggcttaaaa attctttatc
    16021 cggcatttcc aagtgatgca aaagggttat tagccgctgc tattgaagat cctaatcctg
    16081 taatgttttt tgaacataaa gcactctata gaagtgtcag tggtcttgtg gctgatgatt
    16141 actttactat tcctattggt aaggcaagta aaatcagaga aggtactgat ctaactattg
    16201 tgacatacgg cttgggtgtt cattgggcaa tggaagcgtt ggatgaaaat aaaaatattc
    16261 aggctgattt aattgatctg agaagtcttt taccttggga taaagaaaca gttttagaat
    16321 ctgtaaaaag aacaggtaag gttatcatat ttcatgaaga caccttgacc ggtggaatag
    16381 gaggagaaat tgctgctgaa atttcttctg cttgttttca gtggctcgat gctcctgtga
    16441 tgcgatgcgg tagtcttgat actgccgttc ctatgtcaaa tcatttggag tggaattttc
    16501 tgccacgcga tagatttaag caaatgctga aacaattagc agcgtattaa gataagcgtt
    16561 attttttttc tacagcccat ttaattgctt caccaataga atatttccct gcaatagtca
    16621 taaattcact tgcagttgac cataatccag atgaggattt atgctgtttt aattcttcta
    16681 tcttaatttc atcaaaccaa acattacctg ttccattcag aattcctgta atgctcatcg
    16741 tttgtgctcc ctcaggaaca gaagttgcaa ttgaataagt tttccatttt gcttctgcat
    16801 ttttaataaa accactactc tcagttaatt ttttccctga tgcatctctg attattatat
    16861 taagacctgt attcccattt aatgcttcgg cccggcatga ataaattatt ttgtaatttt
    16921 ttatctctga gtctttaatt gatatactct gactaaatgt acaggtagcc tgattttgat
    16981 gcgctatctc caggcattgt ttgcctgaat gtggagaaat attactgaat tttatctgac
    17041 ctgttccttt acaatcagtt atccatccct tctgtgaaga agaatgagca tcttcttcaa
    17101 aaccactgtt aattattgtc tgagcatgaa gttgtgttac gccaacacat aaaaataata
    17161 tcagaattcg ccttaaatgc atggttgcaa ataaagaaaa ttcatggcaa acattcacaa
    17221 tttgtcgtta atattaatac atctgtaaca agaaaagttt atttataaat tttacatttg
    17281 cttgcaagaa gaatttatta tgtttcgtta tttattacca ctcatttttt tgatttctac
    17341 aacatcttgg gctcagcata aaaaattgag catggaagat gcaatattaa atgcgagaac
    17401 caaacttgca ccggcaaatc tttcacaatt aggctggatt aaaaattcaa tctcatacta
    17461 ttatgttgac aaccgcttgc ctgatgcaac actttttgtt ggtgatgacg gtattgtaca
    17521 tgatgagaaa attatttcac tttctgagat taatgataaa ctaaaacgta ttaaaacgga
    17581 tacacttgca agttttcctg ttgttgaatg gaagaatgat aaagtctttg gattttttat
    17641 tgggaacaaa gagtttgaat atgataaaac acaatatgca attcgtaatt caagaatttt
    17701 attgttgccc gatagcgcta cacaaaaaga caccgaccct gtaacagggc gtatagccta
    17761 tacaattaaa aataaccttt ttgtttatga tgcaggaaag aattttacag tagcagaaga
    17821 tgcttatcca catattgttt acggcagctc tgtacatcgt aatgagtttg gaattacaaa
    17881 gggaaccttt tggtcaccga aagggaacaa actcgctttt tacagaatgg acgaaaccat
    17941 ggttaaagat tacccattaa tagattggaa taaaaagcct gcagaggtaa atctgataaa
    18001 atatccaatg tccggtggca caagtcatca ggtaacagta ggtgtctttg acatgattaa
    18061 taaaacagtt gtttatctaa agacaggtga gccaaaagat caatacctga ctaatgtttc
    18121 atggagtcct gatgagaaat atgtttatat cgctgtttta aatcgcgatc agaaccacat
    18181 gaaattgaat tgttattctg catcaacagg acattttatt aaaacacttt tcgaagaaaa
    18241 agatgaaaag tatgtggagc cacaaacacc tatggcattt gtccccggcc atgatgatca
    18301 gttcatctgg caaagtcagc gtgatggcta caatcattta tatctttaca ataccgatgg
    18361 aaaattaatc aagcaactga caaaaggaga atttgttgtc actgagttta agggatttga
    18421 taaaaaatcg gaacgtgcat attatatgtg cactgcatta agtcccatag acagagattt
    18481 atattatgtt acactttcag atggaaaaat gaaaagagtt tccggtgata gtggtattca
    18541 tacttgctat tataatcctg agaacaatga agtgcttgat gtattcagta atgtaaacac
    18601 acctcgtaaa ataaaactga tactgcctaa tggttctgat gcaaaaacaa tgctgacagc
    18661 agaaaatccg ttaagtgatt ttaatttagg cgaaatgaaa attagtacac taaagagtgt
    18721 ttccggtgaa ttgctttata cacgcaccta ttatccacca tcaatggatt catcaaagaa
    18781 gtatccggtg attatttatg tttatggtgg accacattta caactaatta caaactcatg
    18841 gcagggtgga caaggcgact tgtggtttta ttatctggcg caggaaggat ttattgttta
    18901 ctctgttgat ggcagaggta gtggcaacag agggaaaaat tttgaacaag ccattttccg
    18961 gaattgtggt gtagcagaga tggaagatca gcttacggga ttacaatatt taaagtcttt
    19021 accctatgtt gattcatcaa gaattggtgt tgatggctgg agttatggtg gttttatgac
    19081 gatttcattg atgacaaggt ttccgggatt gtttaaagtg gctgttgcag gtggacctgt
    19141 tattgattgg agtatgtatg aaattatgta cacagaacgt tatatggata ctccggttac
    19201 aaaccctgat ggctatgaaa aaagtaattt attgaactat gtaaagaata ttaatggtaa
    19261 gttgatgctc atacacgaca ccaatgatga tactgttgtt tggcaacata gtttaatgta
    19321 tttgaaaaaa gctgttgatc ttggaaagca agtggattac tttgtttatc ccgggcatcc
    19381 acataatgtt cgtggtaaag atcgtgtaca tctgatgtat aaaattacag attactttaa
    19441 acaaaatctt tgaaaacaaa atttaattaa tgggtaagaa aacaacttat tgtcttttac
    19501 tatgtttctg tgttgtttca ttacatgcac aaaatctaaa acgctgtcca cagacaacat
    19561 caaaagaagc agctaagaaa acagaagcag ctgagaagct attaaagtct gtaaaaaatt
    19621 atgaggaagg agttgaattg tgtaatgaag caatcgaggc cgacagcaca tttgcaaaac
    19681 cttatttgct tatgggtgat gctgcataca gaaaaaaaga ttttaagtta atggcaaagg
    19741 cttataaagg tcttatgaat gtttgccccg aagcatctga aaaagcaatt tacaggctgg
    19801 caacctatta ttttgataca aaaaaatatg aagaagctat tcctgtttac accacatttc
    19861 ttgaactctc tccgtcagat accaacgctg ctgacgatgc cgaagtaaaa ttattcagag
    19921 caaaagcgtt cttacatcct gtagatttta aacctgtgct ggtaaaagga ttaagcacac
    19981 ctgacccgga gtacctccct attatctctg ccgataatga attatgtttt tttacacgga
    20041 ggtttgaata ccaaagtaaa aacatgctta cctcaaccag tgtagaaaag tttatgttga
    20101 gtcataatct aaatggcaaa tgggatacag gaaaaccaat ggatttacct tttaacaaac
    20161 agaatgccgg aaatgaaggt ggtccggcaa tttctcttga caaccttcat ttgtttttta
    20221 ctgttaatgt taaaggtaat ttcgacattt atacttctga ccttacacca aaaggatggt
    20281 ctgaaccaag aagtataggt aaagcaaatg actcaaagtt ttgggattca caaccatcac
    20341 tttctcctga taacaggact ttatattttg ccagtttcag agactctgtt cttcaaacaa
    20401 gtgatcttta tgtgactaaa aaaactaatg gagaatggaa cagcgcaaat cccattaccg
    20461 gacctatcaa tacttcaaga aatgagaagg cacctttcat acatcctgat ggaaaaacac
    20521 tttatttcag cagtgatggt catccgggta tgggaggata tgatatttac tattccaaga
    20581 aagatgcaaa cggtaactgg ggaaaacctg ttaatctcgg atatccgata aacaccgatg
    20641 cagatgaggt tggatttttt gtaagtactg atggtactaa aggttatttt gctagtaata
    20701 aattcggaac cggaggttat gatatttatt cattcgacat gcctgtggat gcaaggccgg
    20761 agaagatggt tctgttcaaa ggccaactga agaatgatga caatgaagtg cctgacagtg
    20821 ctagaataga aattcggaat atttcatcca aacaggttga gcagatagat tacgatgcct
    20881 acagtggtaa atatgccggt gttttgcaag ctaattctga gtatttgcta actattcaaa
    20941 aggatgatta tgcatttact tcaaaactta ttaatgctgc aaaagttgat tcgcctgctg
    21001 tgactgttga gttagacctg aggaaaataa aagaaggaga ggcctacaat ctgaatgata
    21061 tcctttttga aacagattct tatcaaataa cagatatatc taaagcagtt atacaggatt
    21121 ttgccggctt tctgaaaggt aacccaaagc taaaagttgc tatttatggc cataccgata
    21181 attcaggtaa ggctgaggat aatctattat tatctaaaaa cagggcaaag gctgtatatg
    21241 actatttgat aaccttaggt attgaagcaa cacgactttc gcataatgga tttggacaaa
    21301 caaaacctgt tgcacccaac acaacagaaa aaggcaaatc attaaacaga cgtacagagt
    21361 ttgtagtgct aaaaaaatag ttttatggag tttgtgattt tggttgatga gcaggatagg
    21421 gaaataggtg aggcagaaaa aatgcagacg cacaaacttg gattattgca tagagctttt
    21481 tcagttttaa tatataatac aaagggtgaa atgcttttac aacaaagagc agaaactaaa
    21541 tatcatagtg gagggctatg gtcaaatgct tgttgtggtc acccgcgtcc tggtgaaaaa
    21601 attgaggatg ctgcaagaag aagacttaaa gaagagctgg gctttaattg tagtctgaag
    21661 ttcagtttta aatttattta taaagcttct tttgaaaaca gtctgaaaga gcatgaactg
    21721 gatcatgttt ttacagggat ttatgatagc gaagtgcttc ctgataaaaa tgaagtacaa
    21781 gcctaccgat gggttacaat ggaaaattta aaaaatgaaa ttattgaaaa ccatagtagt
    21841 ttttctgtat ggtttcaaaa aattattgca gtgttgtgat tccctataat ttagccatta
    21901 attttgaaat aaattaccct ttcatacgtt ttgcttgaca gcaattactt atttttggtt
    21961 cccatgaata tattcaatat cagctgcaga aattttatcc tgacttgctt aattttaagc
    22021 atgcctgttc tggcatttgc gcagtcgaat caggaaagac ttgttcagtt aagcggtgta
    22081 atagttactg gcgacagttt gagtccggta ccttatacag gcattatgat taagtcaagt
    22141 agcagaggta cagtatctga ttactatggg ttcttttcaa ttgtggcaca tgttggtgat
    22201 gagattgagt tttcttctgt tgggtttgca aaaactaatt ttaaagtacc cgatacgctc
    22261 acagaaaacc gttattctct gattcagatt cttaaccgtg ataccgtatt gcttaatcag
    22321 gctgtgattt atccatggcc tacacgtgaa cagtttaaac aagcatttct cgatctaaaa
    22381 gttcctgata cagacatgga tcatgcccgt aggaatctcg ctcttgaaac tatgcgcgaa
    22441 gagagcatca gaattgatat ggacggaagc atgaacagta aatttgttta tcagaaacaa
    22501 cagtcacggt tatataatac aggtcagtta cctgtaaaca atttgctaaa cccaattgcc
    22561 tgggctaaat ttattcaagc atggaaaagt ggtgagttta agtcaaagtc taaagacgat
    22621 taatgaggca acttgtcatt tttctttcgt tattttttta ctgttattca ttgaacggac
    22681 aagatgcaac tgcacgtatt catggtaaaa tatcaggcac agaccgcaag cctttgcccg
    22741 gtgttagtat tggtgttgcc ggtacttcac tcggtgtaat ttctgatgag catggcgagt
    22801 attccattac cgttccatct aatcgtgagt taagtattgt tttttcgtca attggttatg
    22861 aacaagcaac agtaaaacta attcttgcag aaggtgaaaa gaaacaattg aacagaacca
    22921 tgttgccttc aattgtacca ttgcccaata tcaccattac tgatcctcgc gaaagaagtg
    22981 gtacattaat aaaaatagat ccaaaagcag tagaagaaat tccaaatcca tcaggaaatt
    23041 ttgagaccat attaaagact attggcagtg gtgttgtttc taacaacgag cttagttctg
    23101 aatattctgt gcgtggtgga aactacgatg agaatcttgt ttatgtgaac gatgttgaaa
    23161 tttacagacc acaattggtt cgttccggat tgcaggaagg attaagtttt atcaatcccg
    23221 atcttgtttc cggaattaaa ttttctgccg gtggttttga tgctatttat ggagataaaa
    23281 tgtcatcagc acttgatata aaatacaaac gaccaacaga atttgcagga aaaattactt
    23341 acgggttgct tggtggtggc ctgagccttg aaggtacaag tcgtaataat cgttttactt
    23401 atttgatagg aggaagacaa aagtcaacaa aatatttatt aggtacactc gacactaaag
    23461 gaaactataa gccggtattt tatgatgtgc aggcattgtt gacttatgat gtgagtgaaa
    23521 aaacaacaat ttcattatta ggaaattttt caaagagcag atataatatt gtgccgcaaa
    23581 cacgaacaac agattttggc actgtgagtc aggcattacg ttttactgtc tattttgatg
    23641 gcaaagaagt tgatgacttt actaccacta caggagcaat aatattcagt catagcccaa
    23701 agaaaaatat taacctcaaa gtcattggct cagtatttaa cacaagagaa gaagaaacat
    23761 ttgatgttct tggtcagtat tttttggatg aacttgaaaa agatcctgcc tcgcaaaatt
    23821 ttggtgaagt aaaactcaat cttggtgttg gttctttttt aaaccatgca cgtaattttc
    23881 ttgatgggaa agtttattct gcagaacata aaggcaccta caattatcgt cataatgagt
    23941 tgttgtgggg cattaggttt cagcatgaac agtttagtga tcagattaaa gaatggaatt
    24001 atattgactc atcaggctat tcattaccac atcctcagga tagcgtggga gggagccttc
    24061 catcacagca gcaattatta ctgcaagatg tcatcagtcg tgataacaga ctttcaagta
    24121 acaggttgag tggctatctg caacataatt tttatttcag cagcacatca aattttattc
    24181 tcacacccgg tattcgtttt acctggtgga gtttaaatca tgttttaaat atcagtccgc
    24241 gtattgcact tgcttataaa ccaagatgga aacgcgattt taatttcaga ctttcaggag
    24301 ggttttatta tcaaccgcca ttctataaag aactgcataa tcctgacgga agtttaaata
    24361 cagacttgaa atcacaacaa tctattcatg ttgtaggtgg tacagattat tatttcaaag
    24421 catggggacg cgattttaaa tttgtcacag aagcttatta caaatggttg aacgatctta
    24481 ttccttacaa ggttgacaat gttcgcattc ggtatttagg taaaaacaat tcaaagggct
    24541 atgcaacagg tgttgatttt cgtattaatg gacagtttgt taatgaagca gaatcatggg
    24601 caagtctttc acttttaaaa acagaagaag atattcagga tgatgtctat tacatcagat
    24661 taaatgcacg tggcgatacc ataagaggat atacaattga taagcaagca gtggacagca
    24721 ttcaggttat tccaggatat ttgccaaggt tgaccgatca gcgacttacg ttcagcatat
    24781 ttttttctga ttatcttcct aagtttccaa catttaaaat gcacatgaca cttgttttcg
    24841 gaacaggatt tcctgttggc cctccaggga ataatcgcta tacagacatt aatcgttatc
    24901 ctttttacag acgtgttgat attggattca gtaaaatttt tattgacgaa aacagaagag
    24961 caaaaagtcg ttttactgcg ttaaataagc taaaagcact ttcactttcg cttgaagtgt
    25021 ttaatctttt tcaggttaac aatgttgttt cctacacttg ggtaagtgat gtttatggaa
    25081 ctaagtatgg tgtgcctaat tatcttaccg ggaggcaatt gagtttgcga ctaagtgcta
    25141 agttctgatt gtagttggca taagtttgtt tcctatctac agttaaaatt agtttcctga
    25201 ttcctcatct ttacatcatc agttgatttg ttctttcgtt gagtgagtta ttagaaatct
    25261 tttaaatttg aatttttaat ttttctgctt atgcgtaatc attccgaaag caagttccta
    25321 accgaacgac agtcgcgaat tggtgaattt ctctttactc ttaaagttgt aaaagaattt
    25381 ataaaaggat tcagaacatt gcattttgtt ggaccatgtg ttgcagtctt tggttctgct
    25441 cgttttaagg aagaccatca ttattatcag ttaggccgcg aagtaggggc taatgtcagc
    25501 aggttaggat ttacggtaat gacaggtggt gggccaggtg tcatggaagc agcaaacaga
    25561 ggagcaaaag aagtaggtgg tttttctgtt ggctgcaaca tcattctgcc tcgtgaacaa
    25621 caacccaata agtatcttga taaaatggtt gagtttcatc atttctttgt cagaaaagta
    25681 ttgatgttta agtattctta tgcttttgtg gttttaccag gcggtgtagg aacaattgac
    25741 gaaatgtttg aggcattaac cttgattcaa acaaaaaaag ttggcgattt tccggtagtt
    25801 cttatgggga agcattactg gcaacctgta attgatcagc tgaaagtatt cgatgagcat
    25861 aaaacagcct tacgctctga gttagattcc ttgctgatta cagacagttt agatgaaatg
    25921 acagagtatt tacgtgtaaa ggcaattgaa agatttaaat tgactaaagc catgaaattc
    25981 aaccgctcga aatggtttct tgaagactga gcccgactgt tggcaattat tcaataaaaa
    26041 cttttgcaat tcatttctaa cttatatttt tatcctgtaa tttttctgag tattcacagg
    26101 acttaaatga aagtattccg cctttgtttt tttattttta tttcttcaat gctgcaagcg
    26161 caggatattc atttttcaca gttctataac tctccactta cacttaatgc tgcaaacaca
    26221 ggattgtatg aaggagatta tcgttttgcc aacacttata ggagtcaatg gtcgaaactt
    26281 gatcctggtt acagaacaaa tgcattttca tttgacaaaa cactttatac cggtgctgat
    26341 cagattagtg gtggtttttt gtttatttac gaccgctcgg gaatcaatcg gttacaaagt
    26401 attcgtttag gattatctgc tgcttaccat cgtacgtttg gcgtacacag tttaagtgca
    26461 ggttttcagt ggaattatat ttttaaatct attgacagaa gtaaaattac aatgcctgag
    26521 cagtttaata tgaacaccgg ttattttgat ccatcaatgc ctacaggcga agtattgctt
    26581 gatgaaaaac gttctttcgg tgatattaat gcagggttgg tctatcaggt tgaattaaat
    26641 aaacatttta tccaggccgg tgcttcgttt tttcatctga ataagcccgg tgatactttc
    26701 ttcaacagca ataataaatt gccagttcga acatgcttaa ccgtatcaga taaaatagga
    26761 attggcaatg actatctgct acatgcacgt ttattgatga tgcaacaagt aaaagctaat
    26821 gagtatgttg ccggagttac acttgcaaag caacttagta atggagaatc taagtttaca
    26881 gaggttaatg gtggtttata tgtgcgtacg tcacccggca acggcactga tgcagtaata
    26941 cctgttattg gcattaaata tgatttgttt gatgttgcat tgagttatga cataaatatt
    27001 tcatcgcttc atactgctac tgcaagccgg ggtggatttg aaatttctct catctacact
    27061 gcattaagtt cacgtttgtt taaaataaaa atcccctgtg aacgctatta aaacaaaagt
    27121 tattgtgttg ctggcattag tgttcagtac tgttcattta tttgcgcagc aaaatgatga
    27181 actaaaaaaa tacggtacac cacaattaaa acgaatgggt agctatgctg caaagattaa
    27241 tgacttcagc agttctgcca tgtactatga agagtattgc aagcgcaagc caacaaatga
    27301 gaaagtgtgg tttctgtatg ctgaaagtct tcgtaaatcc aaagattacc gcaaggctga
    27361 agatgcttat atgaaagcat acaacctgaa tccaaagaaa aatactttag cattgtatca
    27421 ttatgccaat gttcaaaaat ctcttggaaa ttacaaaaag gcagaagagt attttacaaa
    27481 gtttaaaaag gaatatacag gcactgataa aagtgtttat actaaattaa ttaaaaatag
    27541 tggtgatgct gccaagtttg cagatagcct aatgcatagc ccttcgagag ttgccattga
    27601 acatttacct gatgatatca acacatcaca tgttgagtca tcaccatttt atataaatga
    27661 ttcaacaatt atctatggct ctctgaagac cgatcaggtt acttttgatt ttaatccaag
    27721 agattcaagt gcagatgagc ctttccgaaa aatttatgtt gccgttcgtg acagtgagat
    27781 atggaaggat gatggtgagc tccccggatt ttataattca aacagtgccc atacactcaa
    27841 cggtgtttac tcgcaggata agagtagatt ttattttacc cgatgcaaaa gaaatcgtca
    27901 gaataaagtt atatgtgcca tctatgtaag tttcctgaac aatggaacat ggacagagcc
    27961 tgtatcttta ggaacagaag taaatgatcc gaaatataca tcaacgcaac ctgccattgg
    28021 tctgaatact cagaaaaaag gcgaagaaat aatttatttt gtcagcgacc gcgaaggggg
    28081 taagggtggt tatgacattt ggtacacctc ttttgataac agaaataaat cttacagaac
    28141 acctatcaac cttggaacaa aaattaatac ttccggtgat gaagtaacgc cttatgttga
    28201 tgcacaaaca caaacatttt atttcagttc cgatggatgg cagggccttg gtggtttaga
    28261 tatctttaag tcaataggcg gaatgaaaaa gtggacacat cctgtcaatt tgggttatcc
    28321 tgttaattcc aactatgatg atttgtatta tgtgctttca ccaagtaaca aagacattgg
    28381 catgatagtg tctaacagaa atgttaccga taatggcgtg aataaaggtc cttgctgtga
    28441 tgatattttt gagtttaata ttttagacgc agtgagatta aatgtgaaag gctttttata
    28501 tgaaaccgat tcaactcaaa aaggaaagaa gttagcaatg atgcctgtac gaagagcacg
    28561 cattactctg gaaatgaaaa atgctgaaga ttctactttt attcctgtca gtaccagtaa
    28621 tacagatgaa acaggtcact tcaatcttat tgtttctcca cgccaggaat atcggattac
    28681 agcccgtaag gaaggttatc tttatgagtc agtggcgttg agtactatga ataagacccg
    28741 ttccggtgat atctcaattg acatacacat acgttcaatt cccgaaacac cactgctgct
    28801 tacaaacatt tattatgagt ttgcaagtcc tttgctgact gcaaaagcta tggctgcatt
    28861 agatactacc ttggtaattt ttatgcaaag aaatgttgac ttaggtgttg aaataaggtc
    28921 gcatacagat gccatcgggt ctgatgatgc caacatgacg ctatcgcaaa aacgggcaga
    28981 aagtgtagtg aattatttag tggcaaaagg aattaatgcc aacagattgc gtgcaaaagg
    29041 atttggcgaa accatgcccg tagctccaaa tacaaaacct gatggaagag ataatcccaa
    29101 agggcggcag ctgaacagga gaacagaatt taaaattttg ggtaaaatgc gtgatggtgt
    29161 catccgcgaa gaaaccgatt tctgatttta tttcttccag gtagtataaa cggcatcgtg
    29221 ttttttacga ttgggttcta tacttcgcaa aggagcacac ggttttaatg tggctcttaa
    29281 ttcatgaggc agttgttgat agagttcagt tccttttgtt ttccaatgta gcatttcatc
    29341 acttacatcc ttaatgattt ttgccggatt gcctacaaca attttcctgt taggaacaat
    29401 cataccgtca ggaacaaaac ataaagcacc aacaatacaa ccatcgccaa ttacggcatt
    29461 atccataatc actgcattca ttcctatcaa agaattttta ccgatggtgg caccatgaat
    29521 tatagcgcca tgtccaatgt gtgctccttg ttttaaatta acagtaacac ctggaaacat
    29581 gtgaattgtg cagttctcct gtatattaca accatcttca ataataattg ttccccagtc
    29641 gccacgtatg gctgcacccg gagcaatata tacctgcttg ccaataatta catcacctgt
    29701 aactgttgcc tgctcatgaa caaaactact gctgtccacc acaggtctga atccgttaaa
    29761 ttcgtaaatc atttgaataa ctgatgtttc ttgtaacaat gcaaatgtaa agttaattga
    29821 taagccaaaa cattaacaat tgttggtaac tatccgcaag catcaatctt ttagaagtat
    29881 atttttgtgc taaaaccatc attacatgtc aaaatatttt gttgcaatat tgctcacttt
    29941 tacttgttta gcttcatcgg tcagatcaca gacattaaag agtttcaata cagacccttc
    30001 aagctatctt cttgatttaa aatctttttt tgaagaaaca aataagaagg aagcagagaa
    30061 gataatggag gaattcaaac ctgtttttct tagcaagttt gatgtgcagc aacagcagtc
    30121 tattattaca acaagtaatc tgatgttgaa aaaacgcatg aaggcatttc ctgattttgt
    30181 tacctacaca tctgcgctta ctgcctttgc ttccagcggg caagaagcaa caacatttac
    30241 ctcgtggcat gcaacttttg caaaagctat agcaaaactt agtgtaagaa aattgggtga
    30301 ctttcttgaa atatcacaac tgctttttcg taacaatacc ttgtatgaat caagtgctgt
    30361 tacatggagt gcaagtaata acaaatttgc atttggattt gactccttgc caaaagttac
    30421 tttttccggc atgaccttgc gatgcttcgg caagggtgat agctctgtaa ttgaaggcac
    30481 aaaaggagtt tattacccca acaacctttt gttttttggt gatggtggta cagtaaattt
    30541 tacacgtgct ggaataagtg tttcagaagc aaatgccatt gtgaaacgct atgcaatcaa
    30601 tttaaaggga tcagaatatt cgatggactc tgttgcattt acgtataaaa agtattttga
    30661 tcaggaactg aaaggtcgtt atattgataa gttattggct aacgtaaatg attccaatgc
    30721 tacttaccca aggttttatt cttatgctgc gaatctcgat attaagaata tggttaaaga
    30781 tgctgattac aaaggtggat tctcattgca gggaagtaag atggtaggtt caggcaacag
    30841 acgtcaggat gcatcaatta cttttaattt aaatggcaaa cctcagttga aattactttc
    30901 acaaggtttt atttttcgtc ctgacaggat tgtttctgta aatgctgctg ctgttatcta
    30961 ctgggagaaa gattcaattt atcatcccgg agttgaattt aagtatatct atggtgataa
    31021 aaccgtgacg cttacaaaga atggacaaac tgctatcaat agcccgtatt ttgatagcta
    31081 tcataaaatg gatcttgatt ttgatcagct ggtatggaag attacagacc cattgatgga
    31141 ccttaaaatg atttctggtg gtggtgaaag taagttgaaa tttgaatcag taaacttctt
    31201 ttcacgtcaa cgttttgata aaatccaagg actgtctgaa gtgcatccgt tgtttaaaat
    31261 taagcaattt gcagaggagc ataatgcaaa agtcatcagt gtacctgaat ttgctgaata
    31321 tatgaaactc actgaaaaca cagtgcgcaa tcaaattcta cagttatcat cattaggatt
    31381 tatttcttat gatgccgatg ccgataaatt tattgttaaa gataaggtaa tgtattacct
    31441 gaatgcgcgt accggtaagg ttgattatga tgagttgaag atagaatcca tgatttccgg
    31501 tttatctaat gcaaccatca acttattaaa ttttgatatc aacatgcgtg gtgtaagtca
    31561 tgttttaatc agcgattctc aagaggtata tattcaacct ttggatcagg aaatgacatt
    31621 aaaaaaaaat cgcgacatcg tttttaatgg acgtgttcat gcaggaagac ttgactttta
    31681 tggtaaggag tttactttta actatgccaa ttttaatatt gacttaaaga atgttgactc
    31741 attacgtttt aaagttccat ctgacactgt tgatgaaaga ggtagaaaaa gactgatacc
    31801 aattaaaagt attttacagc aattaaatgg aaaactttct attgataatc caaccaataa
    31861 gtccgggttt gtacccaatg aagaatatcc ggtttttgtt tgtaccaaca aatcatacat
    31921 ttattatgat tatcctgaaa tatttggcgg tgtatataaa cgcgataagt tttattatca
    31981 tctcgatcca ttcagcattg acagtctgaa cacacttact aaggaaggat tagtatttga
    32041 aggtagcatg gtgtcggcag atatttttcc ggaatggcgg cagcaactca ctgtgcaacg
    32101 tgattactct ttaggatttg ttaccaacac acccgatgcc ggatttgcag catacagtgg
    32161 caaaggaaaa tatcgtcagc aaatcagttt gagtcatgag ggttttctcg gaaaaggaga
    32221 ggttgattat ctgagcagta cttcaaaatc taacaatatt attttctttc cagacagtat
    32281 gcatgcagtt acacaaagtt ttgctattag aaaggagcaa gttggtgcta ctcgttttcc
    32341 tgatgtgaag aatgaagatg tgtttgtaaa ctggcgaccc aagagtgata aaatgtttgt
    32401 gtttaagaga agtaaggatt ttgcattgtt taatggagaa gcagtgctcg atggaaattt
    32461 agtgttgatg ccttcaggaa tcaaggccaa tggtataata cgttatcagc aatcacagtt
    32521 aatcagcagc aatcatttta tcagagaaca cagttacggt tccgattctg ctgagtttaa
    32581 acttaataca gatgccggag atttgctggc attacatacg caaaatgtta aggctaatat
    32641 tgaccttgat aaacgtattg gggatttcag tagtcagggt gaaaaagcca aggttgaatt
    32701 tcctgtcaac gaatatatct cctacatcag agatttccga tggtttatag ataagtcgga
    32761 gcttgaattt ggtggttctg atgctattgc tgacaaagca aatgagtttg tttcaataaa
    32821 ccgacaacag gattctttgc gttgggatgc acctttagct acatataatc ttaaatcata
    32881 cttgctaaaa ggatttaagg tgaaggaaat tttagtggca gatgcttcaa tagttccgga
    32941 cttaggaaat gttgttgtag aacgtaatgc agtgatgcga ccattggtta acagtaaggt
    33001 tattgccaat actacatctc gttatcacac tatgcttaat acaaatatta ctattcaaag
    33061 cagaatgaat tattccggag atggtaatta cgaatatatg gatcagacaa aggttaagca
    33121 taaaatcagg cttactcaga ttggagtgga tacatcaaaa cagacatttg ccatcgggga
    33181 aatccctgac tcgtctcgct tttttattag cccgacaatt cagtacaaag ggaagttgag
    33241 aattaatgca ccaaaagaaa atctatattt caatggattt gcaatcgtta accatcactg
    33301 caatactttt gaacgcaatt ggtttggctt tgcaggtgat attgatccta aaggtgtaaa
    33361 tattcctatt caaagtccgg ctaacgagag tttgcaaaaa ctttattcag gaatttattt
    33421 tggcagcgac agtaccatga tgtatcctgc atttttatca atgaaaaagc atagctctga
    33481 tgcagaagtt atttctgctg agggctatct gacttttgac aataatttac aacaatataa
    33541 aatctctggt actatacccg gaaccgattc aaccaagtcg gcaagtgtta ttacaaacac
    33601 tgttattctt gatgataagc gttgtcagtt aataggcgag ggtcgaatta atctcacaca
    33661 ggattttggc cagttgtcaa ttacaaattt tggtatggtt aattattcaa cagttaatga
    33721 ctctataggc tttgatgcag taatggcagc tgactttatg tttaatgatg atgcacttaa
    33781 acaaatggcg gagatgttta attctattcc tgcattgcaa ccttttatgg atgatagacc
    33841 tgtttataga aatgcactta cgaatatgat cggtaagaat aaagcagaaa agctattgaa
    33901 tgaaaccggg ttatatggct ccgcacgaag aatcccttct gaattacaac atacccttgt
    33961 atttagtgaa cttaagtttt attataataa agaacgtgca tcatatattt ctgtaggaaa
    34021 tattggtctt ggagttatag gaaagactta tgtaggaagg atggttaaag gaaaagttga
    34081 gattatgcgc agacgcagtg gtgatgtatt gaatatttat ctggaagcat tgcctgatat
    34141 ttggtactat tttaattatc agcgtggcgt tttgcaagca atttcttcag atgaaaagtt
    34201 caataagcca attgaagaaa tgaaacccga aaagcgtgta gcatcagaaa agggtgggct
    34261 tgagccatac cagtttatgc ttgctacgga gaggaaaaag aatgagtttt taaagcgaat
    34321 taaagatgct gatttgtaaa aatcagaatt agtaaatttt aaatctgatt atttattatt
    34381 atttgatgca acgagaacat cattataagg caaatattgt ttggacaggt aacacaggat
    34441 acggtaccaa agattattta agctatgata gaagttatct tgtaaggatt gaaaataagc
    34501 ctgacttcca ttgttcttct gatccggctt ttcggggtga taaaacaaaa tacaatccgg
    34561 aagaattgtt gttggcttca ttgtcatcgt gccacatgtt gtggtacctg catctatgct
    34621 ccgatgcagg tgttgttgta aaaagctatt cagataatgc taccggcatc atggttgaaa
    34681 cttcaagccg tgccggaaag tttgaatctg ttacacttaa tccatttgta ttaatagcag
    34741 atgattctaa aaaagatttt gcaatttcat tacataatag ggccaatgag atgtgtttta
    34801 ttgcaaactc tatgaatttt aaagtttatc atagacctac tataactatt gaaaccgggt
    34861 gaatgaacgt aggttttcac tttattgaaa atgctgtttt gacattcttt caagactgaa
    34921 acacatatcg ctttatgatt ttttcatatt ttagctgcgt tttggtacta ttatagttaa
    34981 tgtcaaaata aaatataaaa tcttattcaa atgaaaaaca cattctttac aatggcattt
    35041 attctttgtg ctttttttgt gaaagctcaa tcttcaaatg ttattctatt caccgaaaac
    35101 ggtgagaaat tttatgctat tcttaatgga gttcggcaga atacaagtcc tgaaaccaat
    35161 gttaaagtaa cagggttaaa tgctaacttt tacaaactaa aagtgatttt tgaagatacg
    35221 aagttgggtg aaaaggattt taaccttgca tttaatcccg gtatggaaac aacattcagc
    35281 attaagttta attcaaaaaa gaatagctat agcctacgtc ctttaagtga agttccttta
    35341 gcacaggcat tgcctcctgc acctaatcaa actattgttg tttatagtga agttcctgcg
    35401 ccaccacctg ctgctacaac tgtaactcat actactacaa ccacaacaac cgatgggaca
    35461 ccaatgcccg gtgataatgt gaatgtaaac attggtttaa atatgaatga aactggtggt
    35521 ggaataaata tgaatgtttc aggaggaaca acgcaaacta ctacacatac tacaacgact
    35581 acagttacac atactacaac atcagagcca cctgctgcac cacctgtagc acctacacct
    35641 gcacctgttg tttatctgcc tggatataac ggtccttatg gctgccctgt gcctatggca
    35701 ccacaagatt tttctgatct taaacagtca atttcctcaa agtcttttga agacagtaaa
    35761 atgacaattg caaaacaagt tttgagaaat aattgcctgt ttacttcaca ggtaaaagaa
    35821 cttatgggtc tttttacttt tgaagaatcc aaattggatt ttgcaaagta tgcttatggt
    35881 tttacttacg atatcggaaa ttattttaaa gtgaacgata tgtttacatt cgagtcgtcc
    35941 attgatgatt tgaataatta catcaatagt aggcattaag aaatgtgtta tcttgaaaag
    36001 ccgggaacat ttgttgcccg gtttttttat tgcaaattat ttaaagatta ctaaatgttt
    36061 tgtacaactg ttgcaaataa ctttttgcag atattgtgtc tgcggttgca taataaggtc
    36121 ttttgtaaat aaatttttta ttactcaaat cataaacaaa agtacaact
//
LOCUS       DAOQNP010000021        35698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_383_length_35698_cov_9.482058, whole genome shotgun sequence.
ACCESSION   DAOQNP010000021 DAOQNP010000000
VERSION     DAOQNP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 35698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35698
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_383_length_35698_cov_9.482058"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..524)
                     /locus_tag="PKY09_04655"
     CDS             complement(42..524)
                     /locus_tag="PKY09_04655"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNB12179.1"
                     /translation="MNIQSDTSKGPGVYIPPPLFYILIFVIAVFVQKKIPIGDSTLHT
                     LPIKTIGIILFIIALFFLFRSLRQFFLTKNTVIVFKPATSLQTTGIYAITRNPMYVGL
                     AIVYLGATIFIGNWWNIILFPILILIIQEYIIKREESYLAIEFGHEYENYKKKVRRWL
                     "
     gene            complement(681..1397)
                     /locus_tag="PKY09_04660"
     CDS             complement(681..1397)
                     /locus_tag="PKY09_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNB12180.1"
                     /translation="MSLSYISKKAIPKGEIVKGHEIREGIFLLIKSNFPNFDADSTIS
                     IDELNKYRRLYLTKLVEQEKGELATIDLDVMQAIRDNSILSENIQDEIEAKLTTGQKL
                     ADKIASFGGSWTFILSFFFFLLLWIIINIWLLKSKPFDPYPFILLNLILSCLAAIQAP
                     IIMMSQNRQEQKDRQRGEHDYKINLKAELEIKLLSEKIDHLLVHQNKKLLEIQEVQID
                     YLEDLMKQIKINYVNIKKHE"
     gene            complement(1496..>1939)
                     /locus_tag="PKY09_04665"
     CDS             complement(1496..>1939)
                     /locus_tag="PKY09_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12181.1"
                     /translation="SQASISQMWTVQFPLLQPNMGLNWYKQILYYNGGSSWLWGHNGG
                     ESGVSTNMYLDPVNNIGLCVLSNGEGTGINICDELYNYALSLNSATGFSPACLTTGVD
                     DLQPAKQKKLIKIIDLLGRETPLKTNTPLIKIFSDGSAEKFFILD"
     assembly_gap    1940..2039
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2040..2683)
                     /locus_tag="PKY09_04670"
     CDS             complement(<2040..2683)
                     /locus_tag="PKY09_04670"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNB12182.1"
                     /translation="MKHTIVKLFLFIIALSTFSTSVLSQINPNPNATVDAFIQNEMNI
                     QHFPGVSAVIVKDNKIVWINSYGYADVANAIPVQDTTVFLLASVSKVFTGTATMQLYE
                     NNIIGLDDDVNQYLPWQLQIPGFPNDSITFRQLMTHTSSIQDNWTAMVGYYGYPDPTI
                     TLADCMQRYFSTNGSDYNAAANFLAQAPGSVFAYSNMATALNGYLTELVSGMSF"
     gene            2869..3207
                     /gene="ytxJ"
                     /locus_tag="PKY09_04675"
     CDS             2869..3207
                     /gene="ytxJ"
                     /locus_tag="PKY09_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HNB12183.1"
                     /translation="MDFKVLNNINQLAEIDELSKRKKQIIFKHSTRCSVSAFAKKVLL
                     SEFSDDMEKVFDVYYLDLLNYRELSNKIASRYSVHHESPQILIISDGNCIYDASHDDV
                     SLTSAFASVN"
     gene            complement(3215..4549)
                     /locus_tag="PKY09_04680"
     CDS             complement(3215..4549)
                     /locus_tag="PKY09_04680"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB12184.1"
                     /translation="MPSKILLVDDEPYIEDLVRQKFRKQIKENQLEFEYVSNGKIALE
                     KLKNDDSYDLMLTDINMPVMDGLTLLEKLRENNILTKAVVVSAYADLSNIRTAMNRGA
                     FDFITKPIDLADLEATIKKTILEMEMLRKGIEASRNLDDALLQKAEAQQKTLETLLEK
                     EKLILQQNELLETQVQQRTAELQQQKLLVEEKNKEILDSIHYASYLQRAILPSEEALS
                     SFFKESFVLYQPKDIVAGDFYWIEPVENGHIIVVADCTGHGVAGALLSMLGISLLNHL
                     VRDKNITQPAELLNQLRIEVSRSLNQAETHAAEGMDIAVCHINCSAKQITFAGAYRPL
                     VYISGNELKTLSGNKIVIGGNNISPQQEFINQNISFDDATTLYLFTDGYADQFGGEKN
                     KKLMSGKLKELLFEKHNLPMKEQLTFFSDYFKKWRGKNDQVDDVLLLGFRCQ"
     gene            complement(4553..6790)
                     /locus_tag="PKY09_04685"
     CDS             complement(4553..6790)
                     /locus_tag="PKY09_04685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB12185.1"
                     /translation="MYHLRWLLFLLFFLALFYSAQGQNSNIKSSQDTTKNIRINLNHL
                     LSEDEKEQAEADSVIQQYINIDNLLEFTNNWKVKSGDDTLWKEPKFDDSAWDEIADSA
                     RDNKNYLSIVWYRMHFDIDSSLLNRPLAFILHLYGSAAEVYLDGKLLKKFGTVASDLA
                     GEEAVFNANPQPIAIVFSKLKDHVLAIRYSNFHRTSAKISGFNIGQNFIIKFKDLNDE
                     INDALLTTKKSALTVFVAAIFLTLAIVHFIMFLYYRQKITNLYYSLYCIGIFIFAFYT
                     YHLVTSTNYASIAFISKSLQFLAPLMVVPIVAMLHTIFYQRFRKILWIIIGAYGLMII
                     LYFFTTRNAANLVLVILMMISFVEILRVILVSVRKKRDGAWIFAMTLLLPVLLGVVTS
                     ILPDQMSFSGMNFEIKPSQFVFASMILGLPFSVALYLARDFARMGKTLTKQIGEITEL
                     STKTIRQEQEKKQLLENQKTILEFEVKERTREVLDQKNVIEIKNREVTESLHYAKRIQ
                     AAILPDIKKINQSLQDLFILYLPKDIVSGDFYSFAQRDEKIIVAAADCTGHGVAGAFM
                     SMIGTALLNQIVNEKGITEPSEILTQLNDGIVTSLKQRESEMNEGMDIALCTINNKSK
                     TLQYSGANRPLWIIRNNEFQYYKPNKIPIGGQQYNKTDGFMQHNITLEQGDSIYLFSD
                     GYVDQFGGDLGKKFMTKKFRELLLSIQHLSMNEQKEYLLNTLIKWQGKHDQVDDILVI
                     GIKIP"
     gene            complement(6861..8198)
                     /locus_tag="PKY09_04690"
     CDS             complement(6861..8198)
                     /locus_tag="PKY09_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB12186.1"
                     /translation="MKKISIIGAGLVGSLLALYLNKRGYKVTLHERRPDMRKVKIPAG
                     RSINLALSDRGWRGLQGVAIDDAIRQIGIPMTHRTMHSVDGKITTQPYGKDGQGIYAV
                     SRGILNCTLLDLAEKGGVEIHFNQRCTGLMLTSGEMQFEHSENHQKSSHHADLIFGAD
                     GAFAASRLQLQLSTDQFNYSQRYLQHGYKELMFVAKADGSHQLEKNALHIWPRGGYML
                     IALPNLDGTFTVTLFFPLKGNPSFESLHNKQEVKAFFQKVFPDAVPLLHDLEEDFFHN
                     PTGSLVTIKCAPWSFDNKLVLIGDAAHAIVPFYGQGMNCGFEDCSVLNDLLNQFADDW
                     EKVIPEYERMRKPAADAIGELAVDNFIEMRDLVGKPEFLLRKKIEGWFSEKHPDKWIP
                     LYTMVTFSHLPYHEAQARGKKQDAIMNEIMKMSDIENIWNSSMVENRIIDLLK"
     gene            complement(8204..9490)
                     /gene="kynU"
                     /locus_tag="PKY09_04695"
     CDS             complement(8204..9490)
                     /gene="kynU"
                     /locus_tag="PKY09_04695"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNB12187.1"
                     /translation="MQFINSADFAAQLDEQDNLKSFRQQFLFPQHQHKDVIYFCGNSL
                     GLQPKQTRDYVLQELNDWKNFGVEGHFMAKNPWYPYHEFLRDNMALIAGAMPSETVVM
                     NSLTANIHFMLVSFYKPTSSRFKIICEGSAFPSDQYALQTQVKFHGFNPDETIIELHP
                     RSGEYTIRHEDILSTITKHKDELALIFLGNPNYYTGQVFDMAGITQAGHAAGALVGFD
                     LAHGIGNLELHLHDWNVDFAVWCSYKYLNAGPGGVAGAFVHQKHCNNTELIRFAGWWG
                     NDPQTRFTMPHDFIPVKSADAWQLSNAPVLPMAALRASLEIFAEAGVQNLIKKRKLMN
                     DFLRFIIGDIISKTNSEKNIIMITPENESERAAQVSMLCKHNGKKIHGYLTEQGIIAD
                     WREPEVLRIAPVPLYNSFNDIYQFGKCLHEALLLNK"
     gene            complement(9587..10150)
                     /gene="gmk"
                     /locus_tag="PKY09_04700"
     CDS             complement(9587..10150)
                     /gene="gmk"
                     /locus_tag="PKY09_04700"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNB12188.1"
                     /translation="MTKHFLFCGPSGSGKTSIVKYLLAKYHFMRFSISATTRPKRAHE
                     TEGQDYYFMTIENFRNHIEQNHFVEWEQVYEGRYYGTLKSEIERIEAINCSVVFDVDV
                     EGGLKIKQHFGSNLLAVFIRPPSAEDLRERLIARNTETAESLEIRLNKSIKELEYENR
                     FDNIIINDDLEQSCLKAEVLVNTFLSH"
     gene            complement(10147..11019)
                     /locus_tag="PKY09_04705"
     CDS             complement(10147..11019)
                     /locus_tag="PKY09_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNB12189.1"
                     /translation="MIRSMTGYGKASGDYKSKTISVEIKSLNSKFFDLNLRLPHPYKD
                     KEMDLRSLLLKEAERGKMDVMITMESDLSQQENLLNREIIKSLLTDLKAVKKEFSLPD
                     DNLLQIVMQMPQVINTDADEADEDEWKYIASMISQAIEKFNLFRQREGQNLQQDISQR
                     MNLILQLLEQITPFEEGRINAVRSKLINSLKSLGANIETDKNRFEQELIYYLEKLDIT
                     EEKVRLAEHCRYFLSMLNDENNNGKKLGFIMQEIGREINTIGSKANDADIQRKVVEMK
                     DEAEKIKEQLSNIL"
     gene            complement(11066..11974)
                     /locus_tag="PKY09_04710"
     CDS             complement(11066..11974)
                     /locus_tag="PKY09_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB12190.1"
                     /translation="MKATTRAHLSVLFVNLLYAANFSIAKQVMPEFIKPFGFIVIRVI
                     PAAVLFFLAAFLIPPEKIKKKDRNTILLCALFGVAINQLLFFKGLSLTSPINGALIMT
                     TNPVLVLLMAAIILKEPVGWIKYIGISLGIAGAMLLILLGKNFNLQGGNTEGDLYIFL
                     NSVSFAIFIILVKPLMLHYHPITVMKWTFFFGALMVLPFGYNEVTAVAWSSLTFNLWL
                     CIVFVVVAVTFVAYLMNVFALRHLSPSVVSIYIYFQPVFATLISMALGEASPGWLHLA
                     SAALIFTGVYLVSKQNIAGINNTENS"
     gene            complement(12001..12357)
                     /locus_tag="PKY09_04715"
     CDS             complement(12001..12357)
                     /locus_tag="PKY09_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNB12191.1"
                     /translation="MKKVYHLANCTTCQRILKELKPGAEFVLQDIKSEPITEQQLDTM
                     QKLAGSYEALFSRIAMKYRSLGLNQIKLTEKDYKKYILEEYTFLKRPVIILGKEIFIG
                     NAPKTIAAAKAAMQKK"
     gene            complement(12404..13879)
                     /locus_tag="PKY09_04720"
     CDS             complement(12404..13879)
                     /locus_tag="PKY09_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB12192.1"
                     /translation="MRILLTGANGYIGMRLLPVLVEAGHDVTCVVRDTLRFKPSPDLL
                     DKINIIEYDFLEPENAIQHFNNKQFDVAYYLIHSLGDTATTLKEYELRCAGCFVLVAT
                     LTQVKQIIYLSGISNEENLSKHLTARKGVTDVFINSGIAYTIFEAGIIVGSGSISFEI
                     IRGLTEKIPVMIVPRWLKSKCQPIAIRNVMNYLHLSLMNEKAFNRVFEIGGPDVLTYR
                     KMLLEYAAERGYKRLIITLPFGFMGLSATWIYFSTPANYTIAKLLVQSMKNDVVCKET
                     SVREVIPQELYTYRQAIQMAFARIEQNMVISSWTDSASSSLSRLDVNQHIEVPSNGCY
                     KDRKWIEIDKDKAEEVANRFFNIGGQNGWYYADTLWRMRGIIDKLIGGVGMSRGRRSD
                     VDIEPGDTLDFWRVLLVDRKNYRLLLYAEMKLPGEAWLEFSIIHNNNRCILKQTATFR
                     PIGIMGRNYWYSMLPFHFFIFRNMIKQIAGDGQHKIRNTKI"
     gene            14043..15518
                     /locus_tag="PKY09_04725"
     CDS             14043..15518
                     /locus_tag="PKY09_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB12193.1"
                     /translation="MAKRTVKQNTKLKFDTAWQYSAAPETPDHIVLADKYDLFINGKF
                     VAPQSKKYFDTINPAKETRIASVAEASQADVDAAVKAARTAYDKVWSKMPARERGKYI
                     YRIARIMQERARELAIIESLDGGKPIRESRDIDVPLAAAHFFYYAGWADKLDYALPGM
                     AARPLGVAGQIIPWNFPILMAAWKIAPALACGNTVVLKPAETTPLTALKLAEIFRDAD
                     LPPGVVNIVTGAGATGASIVNHPGIDKVAFTGSTDVGKIIQKALAGTTKKYTLELGGK
                     AANIIFEDAAIDQAIEGIVNGIFFNQGHVCCAGSRLFVQESVANEVVRKLKDRMETLI
                     IGDPLDKNTDIGAINSREQLNKIKALVKVGVNEGGDCYQSSCTLPSKGFWFKPTIFTG
                     VAQSSRIVQEEIFGPVLSIQTFRTVEEAVEKANNTPYGLSGGVWSDKGSKVFKVTSKI
                     KAGVLWANTYNKFDPTSPFGGFKESGMGREGGMHGLSAYLK"
     gene            15537..16457
                     /locus_tag="PKY09_04730"
     CDS             15537..16457
                     /locus_tag="PKY09_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB12194.1"
                     /translation="METKQKEKIKKVAVGLNGHSHHAETEVRLAVQKTYKLYIDGKFP
                     RTESGRYYKLNDKNGKVIANICRASRKDFRDAVVVARKAQGEWQKKSAFNRSQILYRI
                     AETLETRRQQFVSTLGLQGVSDAAAQKEVSAAVDMLVYYAGWCDKYQQVFSCVNPVES
                     SHFNFSFPEPTGVVSAIASQQLGLLNLVSLMAPALAGGNSIIILAAEKFPLTAIDFAE
                     VLNASDVPGGVVNILTGFSNELHTHFSSHMDVNAIVYADNNSAALKTIQTNAASNVKR
                     VVVVQHVAEESPYRIMELQEVKTTWHPVGI"
     gene            16694..18070
                     /locus_tag="PKY09_04735"
     CDS             16694..18070
                     /locus_tag="PKY09_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB12195.1"
                     /translation="MKVIAADDLSATSLSDSSRLRNIIFILLYFSQGIPEGLTLFAIP
                     AWLAFNGKSAVEIAAYSATVMIPFSLKILLAPIIEKYTYLPMGRRRPWLLFGQFGILS
                     GLFALAFVPEPLDNIFVIVCVVLCLHIFVMFQDIATDSLVIDIVPVEQQGRANSLMWG
                     SKTIGSSVSLFLGSWLINGYGFTSAVLILSAPILVVMFVPLLLREHKGEKLLPWTQGQ
                     TSDETKQFVVESWKKMFKSFKQVVLLRNSILLLLAVFIALGAIYFINTLLPIFTIQEL
                     GWTNVYFSKIFSSCRLIGGVAGMLVGALVIQRMGVIRFIQSSLILMIVIAVLFVMGAV
                     FWHHLNIIVGFIVTFCILATLINIGVLALAMQLCWKRVSAIQFTFCMTIFNAGLATGA
                     ALLGWLRPYMEWQQLFLVFAGLIAFAMVLFKFMKTTNHVKQVERLEEQYMDVLKVEGS
                     LLVKSETI"
     gene            complement(18105..19457)
                     /locus_tag="PKY09_04740"
     CDS             complement(18105..19457)
                     /locus_tag="PKY09_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNB12196.1"
                     /translation="MSNNHLLKSLLLAVILIAGCKPELKELKFDNNSIDFSKFVAIGS
                     SYTAGYQNNALFDMGQQNSFAAILAGQLKKVGGGEFLQPLVSGNDGAGFNFFLQTYIP
                     QTKLNYGTTCNATPGFFTFPSNVTDIVAVETYIGNNAPYNNMGVPGMKSSDLDNANFG
                     STDLLLSKNPFYSRFATNPETSTVLTDAVRQLPTFFSFWVGFNDIYDCAMAGSNQTLA
                     TITPLANFQNNINNALTSLIAGNRKGVVANIPDLLNLPYFTSIAYDALVLTEQQAQDL
                     NTLYASNNPPITFQAGNNPLVISDPSAASGRRQMKPGELVLLPVTLDSINCAGWGSNT
                     QKPLGNRFVLTTSEINTLRNTITSYNNYLKTKAEENNLAYVDMYAFFKKISSGYPHNG
                     VTYSSEFLKGQFFSLDGITPTGRGSALIANEFIKAINEKYQTRIPQTDANSFPGILLP
                     "
     gene            complement(19482..20705)
                     /locus_tag="PKY09_04745"
     CDS             complement(19482..20705)
                     /locus_tag="PKY09_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNB12197.1"
                     /translation="MKKFISGFLILFLFCNLHVSGGGFQMLSQSHSSAGMAGAVIALQ
                     TDGSVIFYNPGGMVFLEKSNVFGGISYSQSRIAFRSNNSGLVDRTESKNMLPFYLGGT
                     IKLSEKMAAGIVINTPFGYNTTWKSDWEGRYIVLSAKLNTLFIQPTFSYKISEKLGIG
                     AGFVFASANYKWNRALIVADGADAQFDGKATGFGFNAGINYAINKKLTAALTYRSKVK
                     LNFKNTDVSFSNIPASLANQYPASANATAEMPLPSVLSIGFSYEVIRGLKLNFETNLN
                     GWSSFDSLNYEFDAPNTTLNDEFRQARKYRDALSLRLGAMYKISDKVTGRAGIAFDQT
                     PVKENYMTPDMPDGDRYVFGIGASFNVSKTFVIEGSYSFETKAEREGINKDTGLNGAF
                     NTLSHCFGVGVNYSF"
     gene            20908..22833
                     /locus_tag="PKY09_04750"
     CDS             20908..22833
                     /locus_tag="PKY09_04750"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNB12198.1"
                     /translation="MLVKKLFLILFSVATTLTCAASVGDTTFVSSHTNDTVVTNPNTG
                     SNGYANWAVFPSTSTNYRKIILTLTHRCPSPMACGEWDYLDYIYIRRTGSQNNPSQDI
                     ELARYITPYGNTFNSTFKSSWTLDVTDFASFLHDSVEIEYIHTGYETNVGRGWLVSVD
                     FALIEGTPVMNPIAFKRLWHGSFPYGNATNPIENYLGADTVTLNFNTANLRLKVTQSG
                     HGADANYCAEFCSKTRTIYFDNSLVDTKQVWRLCGTNPIYPQGGTWVYDRANWCPGNW
                     VYPSVNNFAVAGGSTHIVDMNMQNYNVTSPSANYVIEGQLFEFGPLNNSIDAAIDEIL
                     APTDKFEFSRSNPTCNNPKIRIKNNGSTPITSATIKYGLVGEPESTFNWTGTINTFAT
                     TDVDLPGLISYTSGNRIFKAYIADVNNTADQYVYDDTAYSNAYVPEVMDTVFILLVKT
                     NNYPTENGYTLYDENNNIIVQRLAGTLAANTIYRDTIHATPGCYRYEITDSGDDGLYW
                     WANSAQGTGYHRILRLNNSIARNFERDFGSRYSYRFVSSPGAIVGINNQERYADIVVY
                     PNPTKGIINADITLTSTSPLEVSVYNPLGQLVAEQSRSNFNSGIISFDFKNQPKGIYL
                     VKVVANDQVKVQRVILE"
     gene            22933..24267
                     /locus_tag="PKY09_04755"
     CDS             22933..24267
                     /locus_tag="PKY09_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HNB12199.1"
                     /translation="MKRSYQFSLTLFLMLFALFANAQKHITLEQFTAIDLRGSLNAVY
                     KLDSTWAIEVPEDNVTTQWNLENKTLVLNGAGSNIVVRSPDLEKINISGSGSLSAEDT
                     MELHDLNIDISGSGKVKMNYVKAKNIEANMRGSGKIILGGSAQSLNANLAGSGKIITH
                     KLNVDRAEVNVSGSGTASVWVSDSLTANIAGSGNLYYVSSPRTINQAIAGSGKIEPIT
                     PAIESELNSNSISITTSNKSGTYWSGFELGFNGLYNSKSKFDTPDGYDFLEMIPEKSL
                     AVNINFYEYEVKLIKRYVLASTGIGLSYNNYRFRKNMSLIPDTNALLYTLDTMNLRKN
                     KLTVSYATVPVLLTFNSSSNLKKAFHITTGLLFSYKLGSHTKKVYNKDDKKEKDKTFD
                     DFNIDPFRYDATIRIGYSDFTVFANYALSELFKSGKGPELHPWTIGVSLLPW"
     gene            complement(24338..25498)
                     /locus_tag="PKY09_04760"
     CDS             complement(24338..25498)
                     /locus_tag="PKY09_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB12200.1"
                     /translation="MSRILIIDDEKSIRHSLKEILEYENYKVDEAPDGIEGIKMAEKE
                     KYDIIFCDIKMPRMDGIEVLENLQSKFPEVPVVMISGHGNIDTAVEAIRKGAFDFIAK
                     PLDLNRILITVRNALDRTVLVKETKTLKRKMSRTNEMIGESEGIRKIKDMIDRVAPTD
                     ARVMITGSNGTGKELVARWIHEKSSRNSMPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKTRSGKFEQANGGTIFLDEIGDMSLSAQAKVLRALQENRITRVGGEKDFPVDVRII
                     AATNKDLKSEIEKNNFREDLYHRLSVILIHVPSLDDRKEDIPVLTNYFLKEIADDYKS
                     PAKEIAPDAIEQLQKKNWTGNIRELRNVVERLVILGGKTITKDDVINYVGVK"
     gene            complement(25520..26452)
                     /locus_tag="PKY09_04765"
     CDS             complement(25520..26452)
                     /locus_tag="PKY09_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNB12201.1"
                     /translation="MAIAVIDCGTNTFHLLIADISDDSFKILFRKNLAVKLGEGGISS
                     NKIAREAFNRGIDALKEFAADMKQFVIDKQLCYATEAIRRATNGNDFVEDAKKQSGIT
                     LEIIDGLREAELIYSGVSHAVPFGKEKVLIMDIGGGSTEFVIAGNGNILWRQSFPLGA
                     ALLLDMFKPSDPIHDVQVKLITEHINSVLLPLAEALKQHGNVNTLVGSAGSFDTFLDL
                     CLHRFHRAKTNTTWWSMELEEFFDVHQILLTSTVAQRLEMPGMIAMRADMIVVASLLL
                     HYVIHKHNMNKLLVSAYALKEGMLYFAMNKQSQQ"
     gene            complement(26458..27774)
                     /locus_tag="PKY09_04770"
     CDS             complement(26458..27774)
                     /locus_tag="PKY09_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB12202.1"
                     /translation="MNIILLIIQREYLTRVKKKSFIIMTILGPLLMGGLIAGFAYLSM
                     NQGDSISMIRVVDETGVLAGKLTNSKTVIFTKDTMPIQTARHLFNPNTDYGILYLPAN
                     TLQKPDATVLYTEKQPNLTVVGYLESELSKNIENMKLTDAGIQKSTLDSIKTEISLQQ
                     KPVDPSGKDKSFSAGVTAGIGFGAGLLIYMFIFMYGIQVMRGVIEEKTSRIVEVIISS
                     VKPFQLMMGKIIGIALVGLTQFLLWIILTMAISGTATSLIVGKDQSKLMEMQQKQMTQ
                     SSNIPKETMQPASMDFMKIFEGFNITKILLCFVFYFLGGYLLYSALFAAIGAAVDAET
                     DTQQFMLPITIPLIFAYVVSTIVMTNPESELGYWFSIIPFTSPIVMMVRMPFDPPLID
                     IAISMVLLVAGFIFTTWIAAKIYRTGILMYGKKPTYKELFRWLFYK"
     gene            complement(27771..28700)
                     /locus_tag="PKY09_04775"
     CDS             complement(27771..28700)
                     /locus_tag="PKY09_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB12203.1"
                     /translation="MNLLSIREVSKIYANHKALDSVSIEIPESSVFGLLGPNGAGKTS
                     LIRIINQITGPDGGSILFGGQTLNPSHISAIGYLPEERGLYKKMEVGEQAIYLARLKG
                     LSRADALKKLRYWFEKFEMQSWWRKKVEELSKGMQQKVQFIVTILHEPRLLILDEPFT
                     GFDPINANLIKDELLGLRQKGTTIILSTHRMESVEELCTHIALINKSKKILDGQVKDV
                     RKQFRSNLFEIEYKGTSVGFANALWTGFELVETRTQDDHAFAIVKNLNNSSANDLLRQ
                     LLNDVEVISFRERIPTMNDIFIESVQNNKPLEA"
     gene            28834..30915
                     /locus_tag="PKY09_04780"
     CDS             28834..30915
                     /locus_tag="PKY09_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12204.1"
                     /translation="MRLLSFVLCFMFLSVSVWAQQEELDSLQQLLHKQADDTSKVNTL
                     NELSYVYYQTAVYDSELYYAQQALNLSQKLNWKRGMAVSLKNIGSACDDMGNNSDALY
                     YYGRALNIFSSIDDKSGVSACYNNMGLVYRTIGNFSEALKNHYASLKIKEAIGDRNGI
                     ALSYNNIGVVLNAQKNYAEAIKNHLAALEIRKALGNKRGIASSYGNMGNVYQAQGNYA
                     EALKNYEQTLKIEEEIGNKVGMTTALNNMGAAYYELGDYEQALQHNTDALRIAEEAGD
                     LSSIAASSINTGNVLVKLKQPQAASEWLQKALEIGKEIGEVEVIKNSYEGLSQADSAL
                     GKYNDALQHYKMFISYRDSLINEENTLKTVQLQMQYEYDKKDAIARAVQEKKDEVTRQ
                     EVQKQKLLRNSFIAGFALMLALAAVSYRSYVRKREDNLLIEKQKLLVEEKNKEITQSL
                     NYARHIQAAVLPNHSNINALLKNYFILYLPKDIVSGDFYSVLQNNGRVIVAAADCTGH
                     GVAGAFMSMIGTSLINQLVNEKNITTPAVILDELNEGIINALKQRQGFSNDGMDIALI
                     CLDHQKKQLQFAGANRPLWLFRNHELLVVKPDKYPIGGMQVAHTTQFTNHVIELQTGD
                     SCYLFSDGFADQFGGEQGKKMMSKKFKQYLQVMQHLPMDKQKEQLEQLFNSWKGNYAQ
                     VDDVLVVGIKI"
     gene            31027..35418
                     /locus_tag="PKY09_04785"
     CDS             31027..35418
                     /locus_tag="PKY09_04785"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12205.1"
                     /translation="MFKNLFFSRRLNIAALFLAAFLFNLQSSVGQTSTQNFGTTTGSH
                     TSGTGSSSFIPNPTSGTTYARGGATAPNAPVNLENTSNPLGTTGSFVRAVASSSTSVT
                     KVSPAIAYTAGKEFYTSMKVLFGDASASNTANSGEWAFFQGDGAMYNNNSGFTGNQVF
                     TGLRFTYASGVVNLDYRSGGSWSTSGLLTTSFNQGTTYIIEIVGNNKSSGTINYTYNG
                     NPKSVAIDKFDLYINGTEVGDDLSKAQLTNNTDIKSTTFTGQSSASNVANLFLDDVVI
                     YNSVPAVIGSGSSPILSVGSLSNQFGNVCTGTTTAYETFTVSGANLDGSSVDVGTLSG
                     FSYCLTPNGTYTSTLNLPYTAPTLNSTTVYVKFSPTLVQSYTGTIPVTGGSAAASGCS
                     VTSAAGINTMPSLTTGAATGVTQNTATCEGTITDNGCTAVSAYGIEYSTTNNFTPGTG
                     TQVSSSNISSNTYTSALSGLASSTTYYYRAYATNTAGTGYGAQASFTTDAINAPVAVA
                     ATNIGIHSFRANWNTVSGATGYFLDVSTASTFMAPITTDLVYWDFPNSPDDATADGGI
                     LANVSATISTQGGTSAITYSAGATTEAASATGWDGGNGTKYWEINFDATNYYSLAFSS
                     KQRSSGTGPRDFKVQYKIGSGGTWTDVTGGAVTVGNDFTTGVLTDIALPTACDNQSSV
                     YLRWIMTSNTSVGNGTVASTGTSRMDDLFVTGYEGDFVVNNQSEAGLFYYVTLLSPST
                     TYYYRVRATDGVLTSDNSNVINLTTLGVCDLFTVNASSDAPSPICSTTNINLTATASG
                     SDGTITGYEWSGPGGYSNMTQNPTVVNPVSGTYTVTVTDDNGCTVTDNVSATVETAPT
                     ANAGSDDIICSNGSASLNGSIGGDATGGNWTTTGDGTFSPNATTLNASYIPGTTDISN
                     GSVVLTLTSTGSVLCSADNDQVTITIQTGVPSQPAVVTGAPTSVCPPAGPYTLTTSAA
                     NANTYNWYVVGANTGVTFLSANGSNSMDVQYGVTANSSYSVRVEASNACGTSAYRSAF
                     TRRSVSTPASVSGNKVACASTSDTYSCTAVAGAESYQWSITGDATVSGTTNSVTVNFG
                     PTWTSGTLCVSSKVGCFTSPAKCFVITSSTPSVGTITGNTTACPNGVVSFSVAPLAGI
                     QTYNWTTPANSSVTLGAGTNNVQVTFNGSYNGVGNICVSATSICGVTTAPKCKTVAPG
                     LPSVPASITGGTNGLCNQAGSFSCPLQAGNSYNWTAPGTINNNGSNSVSIQFSNITTG
                     SVCVSASNSCGSSAYRCVTVKGAPNSPTGLTSNPAVICANTSGVEFNANTSNTTGAYT
                     LTWTYTPTAATYNSGGGNTNQLILDWGNTNGVVVVTAHNACGSGAKSYAVTLDCRMEG
                     EASNAIKMYPNPASSSVNVEFNSDKNETVKLIITDLSGKVVMQQSLNVTVGNNTLQAD
                     ISQLAKGIYMAQLSGNNLMSPQKLIVE"
BASE COUNT        10857 a         6769 c         6787 g        11185 t
ORIGIN      
        1 aaagagcaaa ttgttgcaga atacttgaca ggcaacgaca cttataacca ccttcttact
       61 ttctttttat aattttcata ctcatgacca aattcaattg ccaaatatga ttcttcacgt
      121 ttgataatgt attcctgaat aattaaaata agaattggaa ataaaataat attccaccag
      181 ttgcctatga aaatagtcgc gcctaaataa acaattgcta aacctacata catgggattt
      241 cttgtaatgg cgtaaatgcc tgttgtttgt aatgatgttg caggtttaaa aactattacg
      301 gtattcttgg taaggaaaaa ttgtcttaaa cttctgaaca ggaaaaacag cgcaatgata
      361 aaaaggatta tccctattgt ttttattggt aatgtatgca atgtcgaatc accaatagga
      421 atttttttct gaacaaatac ggcaattaca aagattaaaa tatagaatag tggtggcggt
      481 atgtaaactc ccggaccttt tgatgtatca gattgtatat tcattgcgat agaaaatttt
      541 tcaatgtaat attattctgt cattcttgct gtgtgtttta aaatgatttt taaaatcaga
      601 ggttaaagta ttttttatgt ttaataatag aaggaggctt aaataaattt aaagaagact
      661 aattaaccat agcgaaacga ctattcatgt tttttaatgt taacataatt gatttttatt
      721 tgtttcatta aatcttccaa atagtcaatt tgtacttcct gtatttccaa taacttttta
      781 ttttgatgta ccaataagtg atctattttc tcacttaata gttttatctc cagttctgcc
      841 ttaagattaa ttttatagtc atgttctcct ctttgtctat ctttttgctc ctgcctgttt
      901 tggctcatca ttattatagg tgcttgaatg gctgccaggc aagaaagtat taagtttaaa
      961 agtataaatg gataagggtc gaacggttta cttttaagta gccaaatatt tatgataatc
     1021 cataaaagca gaaaaaagaa aaacgaaaga ataaaagtcc aactaccacc aaaggaggca
     1081 attttatcag cgagtttttg acccgttgtt agtttggctt caatttcatc ctgaatattt
     1141 tcggaaagaa ttgaattgtc acgaatggct tgcatcacat ccaaatcaat tgtagctaat
     1201 tcgccttttt cttgttcaac aagtttggtc agataaagtc ttctgtactt attcaattca
     1261 tcaatggaaa tagtactatc agcatcgaag tttggaaaat tacttttaat caaaagaaaa
     1321 atcccttctc tgatttcatg accttttaca atttcccctt tgggaattgc tttttttgat
     1381 atataactta atgacatttt acatttcttt gaatgaagct atgatatttt tttattgtaa
     1441 aagtattggt tatatattat caaactttta attcaattat attttaacat acgcattaat
     1501 ctaggataaa gaacttttca gcacttccat cactgaatat ttttatcaat ggtgtgtttg
     1561 tttttaatgg tgtttcgcgc cccaataagt ctattatctt aatcaacttt ttttgttttg
     1621 caggttgtaa gtcatcaact cctgttgtca gacatgcggg tgagaagccg gtagcagaat
     1681 ttaatgaaag agcatagttg taaagttcat cgcaaatatt tattcctgta ccttcaccat
     1741 ttgacaatac gcaaagtcca atgttgttta ccggatcgag atacatattt gttgaaactc
     1801 cgctttcgcc tccattgtgc ccccatagcc atgaagagcc tccattataa taaagaattt
     1861 gcttatacca gtttagcccc atattgggtt ggagtaaagg aaactgcact gtccacatct
     1921 gactgataga tgcttgcgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2041 caaatgacat accactgaca agttctgtta agtaaccatt taaagctgtt gccatattgg
     2101 agtatgcaaa cacacttccg ggagcctgag caagaaaatt tgctgcagca ttgtaatcgc
     2161 ttccattggt tgaaaaataa cgttgcatac aatctgccaa agttatggtg ggatcagggt
     2221 aaccataata accaaccata gccgtccagt tatcctgaat ggaagaagtg tgtgtcatca
     2281 attgccggaa tgtgatggag tcgtttggaa atccaggtat ttgtaactgc caaggtaaat
     2341 attgattaac gtcatcatca agacctataa tattgttctc atacaattgc attgttgccg
     2401 ttcctgtaaa aactttagaa acagatgcca gtaaaaaaac tgtagtatcc tgaacaggga
     2461 ttgcattggc tacatcagca tatccgtaag aattaatcca gacaatttta ttatctttta
     2521 caataacagc agaaacacca ggaaaatgtt gtatattcat ttcattctga ataaatgcat
     2581 caacagtagc attggggtta gggttaatct gacttagtac agaagtcgaa aatgttgaca
     2641 gtgcaatgat gaaaagaaac aacttaacga ttgtatgctt catgatcttt atattcaggc
     2701 aactgactaa agcttatgtt tgaacaaata tacgaatatt acatttcatg aaaaattgat
     2761 tgtacacttt aattgaaaag caatttgtag caacagttat gattttattt ggtcttccgg
     2821 aataaataaa gtatctttat acctttatta ttaaagagaa tttcatatat ggattttaaa
     2881 gtgttgaaca acattaatca gttggcagag attgatgaat tgtcgaagcg taaaaagcaa
     2941 ataattttta aacacagcac acgctgttca gtgagtgcat tcgccaaaaa agttttgttg
     3001 tcagaatttt ctgatgacat ggaaaaggtt tttgatgttt attatctcga tttacttaat
     3061 tacagagagt tgagtaataa aattgcaagt cgctatagtg tgcatcatga gtcaccacaa
     3121 attctgatta tttctgacgg aaattgtatt tatgatgctt cacatgatga tgtttctttg
     3181 acatcagcat ttgcatcggt caattagaac cgatttactg acagcggaaa cccagcagaa
     3241 gcacatcatc aacctgatcg tttttgcctc gccatttttt aaaatagtcc gaaaagaaag
     3301 ttaattgctc tttcatgggt aagttatgtt tttcaaaaag cagttctttg agtttgcctg
     3361 acataagttt cttatttttc tcacctccaa actgatcggc ataaccatca gtaaaaagat
     3421 acaaagtagt agcatcatca aatgaaatgt tttgattgat aaattcctgt tgtggcgaaa
     3481 tgttgttgcc cccaataacg attttatttc ccgaaagtgt tttaagttcg ttacccgaaa
     3541 tatacacaag tggacggtag gctcctgcaa acgtaatttg ctttgcactg caattgatgt
     3601 ggcatacagc aatgtccatt ccttcggcag catgagtttc tgcctgattg agcgaacgtg
     3661 aaacttcaat tcgtagttga tttaataatt ctgcaggttg ggtaatattt ttatctctta
     3721 caaggtgatt gagcagtgat attcccaaca tacttaacaa ggcacctgca acaccatgac
     3781 ctgtacaatc tgccactact ataatatgac cattttcaac aggttcaatc caataaaaat
     3841 caccggcaac aatatcttta ggttgataca atacaaaact ttctttgaag aaagaggata
     3901 aagcttcctc agaaggtagg atggcgcgtt gcagataact tgcatagtga atggagtcaa
     3961 gtatttcttt gtttttttct tctaccagaa gcttttgttg ttgcagttcg gcagtgcgtt
     4021 gctgaacctg tgtttcgaga agttcatttt gctgaagtat taatttttcc ttttctaaaa
     4081 gtgtttctaa tgtcttttgt tgtgcttctg ctttttgaag taacgcatca tcaagattgc
     4141 gtgatgcttc aataccttta cgaagcattt ccatttctaa aatagttttc ttgatggtag
     4201 cttcaaggtc ggcaaggtca ataggtttgg taatgaaatc aaatgcaccc cggttcatgg
     4261 cggtacggat gttgctcagg tcggcataag cagaaacaac aacagcttta gttagaatat
     4321 tattttctct gagtttttca agtaatgtca acccatccat cacaggcatg ttgatgtctg
     4381 ttagcatcag gtcatagcta tcatcgttct ttagtttttc taatgcaatt ttaccattag
     4441 acacatattc aaattcaagt tgattttctt taatctgttt tctgaatttt tgtctgacta
     4501 aatcctctat ataaggctcg tcatctacta ataatatttt acttggcata agttagggta
     4561 tttttattcc aattactaat atgtcgtcaa cctgatcgtg ctttccttgc catttaatca
     4621 atgtattcaa taaatattct ttctgttcat tcatactcag atgttgaata gacagtaaca
     4681 gttcacgaaa cttttttgtc ataaactttt tacccaaatc accaccaaac tgatctacat
     4741 agccatcaga aaaaagatag atggagtcac cttgctcaag tgtgatgtta tgttgcataa
     4801 aaccgtcagt tttattatac tgctgtccgc caatgggtat tttatttggt ttataatact
     4861 gaaattcgtt attacgaata atccagagag gtctgttagc acctgaatat tgtaaggttt
     4921 tacttttatt atttatagtg caaagtgcaa tatccatacc ttcattcatc tcactttctc
     4981 gttgtttgag tgatgttaca atgccatcat tcaattgtgt tagaatttcg gatggttctg
     5041 taatgccctt ttcatttaca atctgattga gcaatgcagt tccaatcata ctcataaaag
     5101 cccctgctac tccatggcct gtgcaatctg ctgctgctac aattattttt tcatctcgtt
     5161 gagcaaaact gtaaaaatca ccactgacaa tatcctttgg aagataaagt ataaacaaat
     5221 cctgaagtga ttggtttatt ttttttatgt caggtaatat tgcagcctga atgcgtttgg
     5281 catagtggag gctttctgtt acttcgcggt tttttatttc aattacattt ttttgatcca
     5341 atacttcacg tgtgcgttcc ttgacttcaa attcaagtat agttttctga ttctcgagca
     5401 attgcttttt ctcttgttcc tgcctgatgg ttttagtaga aagttcagtt atttcaccaa
     5461 tctgcttggt taatgttttc cccattcgtg caaagtcgcg cgcaagatac aatgctaccg
     5521 aaaatggcaa gccaagtatc atacttgcaa aaacaaactg tgatggttta atttcaaaat
     5581 tcattcctga aaaactcatt tgatcgggta atatgctggt cacaactcct aataatacag
     5641 gtaacaataa tgtcattgca aaaatccaag caccatctct ttttttcctg acagaaacta
     5701 atataacacg aagaatttct acaaacgaaa tcatcatcag aataactaaa accaaattgg
     5761 cagcatttcg agtagtgaaa aaataaagta ttatcattag accataagca ccgattatta
     5821 tccataaaat tttacgaaat cgttgataaa atatggtgtg tagcatggct actatcggca
     5881 ctaccattaa tggtgctaaa aactgaagtg acttagatat aaaagcgata gacgcataat
     5941 tggtggatgt taccaagtga taggtgtaga aagcgaaaat aaaaatacct atgcaataaa
     6001 gactgtaata caggttggtg attttttgcc tgtaataaag aaacatgatg aaatgtacta
     6061 tagccaaagt tagaaagatg gcagcaacaa aaacggtaag tgcacttttt ttagttgtta
     6121 gtaaggcatc attaatttca tcattcaagt ctttaaattt aatgataaaa ttctgaccaa
     6181 tattaaatcc acttatttta gcactagtcc ggtggaagtt tgaatatcga atagccagaa
     6241 catgatcctt taatttcgaa aatacaatag ctatgggttg aggatttgcg ttgaaaacag
     6301 cttcttcacc agctaaatct gaagcaacag tgccaaattt ttttaaaagt ttaccatcta
     6361 aatagacttc ggctgcagag ccatataaat gtagtatgaa tgctaaagga cggtttaata
     6421 aactactgtc aatatcgaaa tgcatacgat accatacaat agaaagatag tttttattgt
     6481 ctcttgctga atcagcaatt tcgtcccatg cagaatcgtc aaactttggt tctttccata
     6541 aagtatcatc gcctgattta actttccaat tgttagtgaa ctcaagaagg ttgtcaatat
     6601 ttatatactg ttgtatgaca gagtcagctt ctgcctgttc cttctcatct tcagaaagta
     6661 agtgattaag gtttattcta atgttttttg tagtatcctg cgaggatttt atattgctat
     6721 tttgaccttg ggctgaataa aagagtgcta aaaaaaataa taagaaaagt aaccatcgca
     6781 ggtgatacat ctgttaattg aatttgcagt aaaggtaaat aaataactac ttatggaatg
     6841 ttcgctaacc ttttgcctga ctattttaac aagtcaataa tcctattttc taccatgctg
     6901 ctattccaga tattttcaat atcagacatt ttcataattt cattcattat ggcatcttgt
     6961 ttttttcctc ttgcctgtgc ctcatgataa ggaagatggc tgaatgtaac catagtataa
     7021 agtggaatcc atttgtcagg atgtttttca ctgaaccatc cttcaatttt tttacgcaat
     7081 aagaactcag gcttaccaac taagtcgcgc atttcaataa aattgtcaac ggcaagttcg
     7141 ccaatggcat cggcagcagg ttttctcatt cgttcatatt ctggtattac tttctcccag
     7201 tcgtcagcaa actgattgag caggtcgttc aacacactgc aatcttcaaa accacagttc
     7261 ataccctgac catagaatgg cacaatggca tgtgctgcat caccgataag aaccaactta
     7321 ttgtcaaaac tccatggggc acacttaata gttaccaatg aacctgttgg gttatgaaaa
     7381 aaatcttctt caagatcatg cagcaaagga acagcatcag gaaagacttt ttgaaagaaa
     7441 gctttaactt cttgtttgtt gtgaagtgat tcaaaagaag gatttccttt taatggaaag
     7501 aaaagtgtaa cggtaaatgt tccatcaaga ttaggaagtg caatgagcat atatccgcca
     7561 cgaggccaga tatgaagtgc atttttttca agttgatgac tgccatcagc tttggcaaca
     7621 aacatcagtt ctttatagcc atgttgcaga taacgctgcg aatagttgaa ttgatctgtt
     7681 gatagctgaa gctgaagtcg tgaagctgca aaggcgccat cagcaccaaa aatcaaatca
     7741 gcatgatgag aagacttctg atgattttct gaatgttcga attgcatttc tcctgaagta
     7801 agcatcaggc cggtacatct ctgattaaaa tgaatttcaa caccaccttt ttcagccaga
     7861 tcaagaaggg tacagtttag aattcctctg gaaactgcgt aaatgccctg accgtctttt
     7921 ccatagggct gtgtggtgat ttttccatca acactatgca tggtacggtg tgtcatcggt
     7981 atcccgattt gacggattgc atcatcaata gctactccct gcaaacctct ccagccacgg
     8041 tcgctcaatg caaggttaat acttcttccg gcagggattt ttactttacg catgtcaggt
     8101 ctgcgctcat gaagtgtaac tttatatcct cgtttgttta gatacagagc cagcagtgaa
     8161 cctacaagac cggcaccaat gatggaaatt tttttcatta ttgttatttg tttagaagta
     8221 aagcttcatg caggcatttg ccaaattgat aaatgtcgtt aaacgaattg taaagaggaa
     8281 caggagcaat gcgtaacact tcaggttcgc gccagtcggc aatgatgcct tgctcggtca
     8341 gatagccatg aattttttta ccgttgtgtt tacataacat actcacttgt gcagcacgtt
     8401 cgctttcatt ttcaggcgtt atcatgataa tatttttttc tgagtttgtc ttgctgataa
     8461 tgtcaccgat gataaatcgc aagaagtcat tcatcagctt gcgttttttt atcaggtttt
     8521 gtacgcctgc ttcagcaaaa atttctagtg aagcacgcag tgctgccatt ggaagcactg
     8581 gtgcattgct caattgccat gcatcggcac ttttaacagg aataaaatca tgcggcatgg
     8641 tgaagcgtgt ttgcggatcg ttgccccacc aacctgcaaa acgaatcaac tcagtgttgt
     8701 tacaatgttt ttgatgtaca aaagcacctg ccacaccacc gggtccggca ttgaggtact
     8761 tataactgca ccacactgca aagtcaacat tccagtcgtg aagatgcagc tcaagattac
     8821 caatgccatg tgctaaatca aagccaacta atgcacctgc agcatgccct gcctgagtta
     8881 ttcctgccat atcaaacacc tgacctgtgt agtagttagg gttacctaaa aatatgagcg
     8941 ccaattcgtc cttatgtttt gttatcgttg aaaggatgtc ttcatgtctg attgtgtatt
     9001 ctccgctgcg aggatgcaac tcaataatgg tctcatcagg attgaatcca tgaaatttaa
     9061 cctgtgtttg taatgcatac tgatcagatg ggaatgccga tccttcacaa attattttaa
     9121 aacgacttga ggtaggtttg taaaacgaca ccaacatgaa atgaatattg gcagtgagtg
     9181 aattcataac cactgtttct gacggcattg cacctgcaat aagtgccatg ttatctctca
     9241 gaaattcgtg atagggatac cacggatttt ttgccataaa atgtccttca acaccaaaat
     9301 ttttccagtc gttcaactcc tgcaaaacat agtcgcgggt ttgtttgggt tgaagtccaa
     9361 gggagttgcc acagaaataa ataacatctt tatgctgatg ttgtgggaaa agaaattgct
     9421 gtctgaagga ctttaaatta tcctgctcat caagttgggc agcaaaatca gcggaattga
     9481 taaattgcat gtttgtgatt taaatgcaaa taaacaaaaa acattttcga ctgacaggct
     9541 atctgttcag acaattctaa aaaaattagc tcttgtggtt taaagcttag tgacttaaaa
     9601 atgtattaac caatacttca gcttttaagc atgattgttc taaatcatca ttgataataa
     9661 tattatcgaa gcgattttca tactcaagct ctttaattga tttattgagg cgaatttcaa
     9721 gtgattctgc tgtttctgta ttgcgtgcta tcagccgctc gcgaagatct tctgctgaag
     9781 gtgggcgaat aaacacagca agcaggttgc tgccgaaatg ttgttttatt tttaatcctc
     9841 cttcaacatc aacatcaaaa actactgaac agtttatggc ttcaatgcgc tcaatttctg
     9901 attttaaagt accataatac cggccttcat atacctgctc ccattcaaca aaatgattct
     9961 gttcaatatg attacggaag ttttcaatgg tcatgaaata ataatcctgt ccctctgttt
    10021 catgcgcacg ttttggacgt gtagtagctg atatgctgaa ccgcatgaaa tggtatttcg
    10081 ctaacagata cttaacaatg ctggttttgc cactaccact agggccacag aaaagaaaat
    10141 gctttgtcat aaaatattag agagctgttc ttttattttt tcagcttcat ctttcatctc
    10201 aacaactttg cgttgtatgt ctgcatcatt ggctttactg ccaatagtat ttatttcacg
    10261 acctatttcc tgcatgatga agcctaattt tttaccatta ttattttcat cattcagcat
    10321 agataaaaaa taacggcaat gttctgcaag tctcactttt tcttcagtga tgtctaattt
    10381 ttcgagataa taaatcaact cttgttcaaa acggtttttg tctgtttcga tattagcacc
    10441 tagtgatttt aatgagttga tcagtttact gcgcacggca tttattctac cttcttcaaa
    10501 aggagttatt tgttcaagca attgtaaaat tagattcatt cgttgcgaaa tatcctgttg
    10561 caaattctgt ccttcgcgct gacgaaaaag attaaatttt tctattgcct gactaatcat
    10621 tgaagcaata tatttccatt catcttcatc tgcttcatca gcatcggtat ttatcacttg
    10681 cggcatttgc atcactattt gcagcaggtt gtcatcaggt aatgagaact cttttttaac
    10741 tgcttttaaa tcggtcagca atgattttat gatttcacgg ttgagcaggt tttcctgttg
    10801 tgaaaggtct gactccatag taatcatcac atccatcttt cctctttcag cttcttttaa
    10861 caacaaactg cgcaggtcca tttctttatc cttgtacgga tgtggcaagc gtaaattaag
    10921 gtcaaaaaat ttactgttga gcgattttat ctcaacggaa atggtcttac tcttatagtc
    10981 accggaggct ttgccatagc cggtcatgga acgaatcata cttcacaaaa ttttggctaa
    11041 agtaatcaga atgacaacag cagcatcagc tattctcagt attatttata ccggctatgt
    11101 tttgcttgct taccaaatat acacctgtaa aaattaaagc agcagaggcc aagtgcagcc
    11161 atcccgggct ggcttcgcca agagccatag aaattagtgt agcaaataca ggctgaaaat
    11221 aaatgtagat actcaccacc gatggactaa gatgacgcag ggcaaataca ttcatcagat
    11281 aggcaacaaa agttactgct acgacaacaa aaacgatgca taaccacaaa ttaaaagtca
    11341 ggctgctcca tgctaccgct gttacttcat tatatccaaa aggtaaaacc attagtgcgc
    11401 caaaaaagaa tgtccatttc atgactgtaa taggatggta gtgtagcatg agtggtttta
    11461 caagaattat gaaaatggca aatgaaacag agtttaagaa aatataaaga tcgccctctg
    11521 tgtttcctcc ctgcaggttg aagttcttac ctaaaagtat tagcagcata gcaccggcaa
    11581 taccaaggga aattccaatg tacttaatcc aacccacagg ttctttaaga attatggccg
    11641 ccatcagtaa aaccaacaca gggttggttg tcattattaa tgcaccattg atgggtgatg
    11701 tgagtgaaag acctttgaaa aacaacagtt gatttatggc aacgccaaag agtgcacaga
    11761 gcaaaatagt gtttctgtct ttctttttga ttttttccgg tggtattaga aatgctgcaa
    11821 gaaaaaacaa aactgcagcc ggaattactc tgattacaat aaatccaaaa ggtttaatga
    11881 attcaggcat cacctgttta gcaatgctga agttggccgc ataaagcagg ttaacaaaaa
    11941 gaacagaaag gtgagcacgt gttgtggctt tcaagcctta ggggtttttc tgtttgactt
    12001 ttattttttc tgcatagctg ctttggctgc cgctattgtt tttggtgcgt ttccaataaa
    12061 aatttctttg cctaaaatga taaccggtcg tttaagaaat gtatattctt caagaatgta
    12121 ctttttataa tctttctctg ttagttttat ttgattgagt cctaatgaac ggtatttcat
    12181 agctattctg ctaaacaaag cctcataact accggcaagc ttttgcatcg tatcaagctg
    12241 ttgttcggtg atgggttcac tttttatgtc ctgtaaaaca aattcagctc ctggtttcag
    12301 ttcttttaat attcgctgac aggtggtgca gttggcaaga tgatatacct ttttcattca
    12361 aatttcttat gtaaaatggt tttacaaaga acatgaaaaa acatcagatt tttgtgttcc
    12421 taatcttatg ttgaccatca ccggcaattt gttttatcat attccggaaa ataaaaaaat
    12481 gaaaaggtag catagaatac cagtaattcc gacccataat acctattggt ctgaatgtag
    12541 cagtttgttt cagaatacag cggttgttgt tgtgaatgat tgagaactct aaccatgctt
    12601 ctccgggaag tttcatttct gcatacaata gtaatcggta attttttctg tcaaccaata
    12661 gtacacgcca aaagtcaagc gtgtcacccg gttcaatatc cacatcactc cttcgtccac
    12721 gactcatacc aacgccacct attagtttgt caattattcc acgcattctc cataaagtat
    12781 cggcataata ccatccattt tgtccaccaa tattaaaaaa acggttggct acttcttcgg
    12841 ctttgtcttt gtcaatttca atccacttac ggtctttata acatccgttt gatggaactt
    12901 caatatgctg attaacatct aagcgactta aactgcttga tgccgaatct gtccaacttg
    12961 aaataaccat gttttgttct attcgtgcaa aggccatttg gatagcttgg cgataggtgt
    13021 atagctcctg aggaataact tctcgaacag aagtttcttt acacaccaca tcatttttca
    13081 tactttgaac taaaagcttt gcaatggtgt agttggcagg tgtagaaaaa taaatccaag
    13141 ttgcagacag acccatgaaa ccaaaaggaa gtgttattat taatctttta tatcctcttt
    13201 ctgctgcata ttcaagcaac attttacgat atgtgagtac atctggtcct ccaatttcaa
    13261 aaactctgtt aaaagctttt tcattcatca atgaaaggtg caggtagttc atcacattgc
    13321 ggatggctat gggctggcat ttagatttta accatctggg cacaatcata accggaattt
    13381 tttctgttaa acctctgatg atttcaaaag aaatactacc agagccaaca ataattccgg
    13441 cttcaaaaat agtgtaggca ataccggagt ttataaaaac gtcagtcaca cctttgcgtg
    13501 ccgtcaggtg tttagaaagg ttttcttcat tgctgatgcc actcagataa atgatttgtt
    13561 ttacctgtgt gagtgtggct accaatacaa agcatcctgc acaccgcagt tcatattctt
    13621 taagtgttgt tgctgtatct cctaatgagt gaatcaggta atatgcaaca tcaaattgtt
    13681 tattgttaaa atgctgaatg gcgttttcag gttccagaaa atcatattca ataatgttta
    13741 tcttatcaag taaatccgga gatggtttaa agcgaagtgt gtctctcacc acgcacgtaa
    13801 catcatgtcc tgcttctacc aaaacaggca gcagtcgcat accaatatat ccgtttgctc
    13861 ctgtaagtaa tatgcgcatg ttttacctga ggttttataa ttattcagtc cttgcaaaca
    13921 tagcaggata tttgatttaa agaaatgata ttgattagta atttttaaat tgcataaatc
    13981 attttcagaa aaaagattga atagaaatga attttctatc tttgaatctt aaattacctt
    14041 aaatggcaaa gcgaacagta aaacaaaata caaagttgaa attcgatacg gcatggcagt
    14101 attcagcagc acctgaaacg cccgatcata ttgtgttggc tgataaatac gaccttttta
    14161 tcaatggtaa gtttgttgca ccacagagta aaaaatattt tgacaccatt aaccctgcaa
    14221 aagaaacgcg aatagcctct gttgcagaag catcacaggc cgatgtggat gcggctgtaa
    14281 aagcagcgcg tacagcttat gataaggtgt ggagtaaaat gccggcacgc gagagaggga
    14341 aatatattta ccgcatagca cgcatcatgc aggagcgtgc ccgcgaactt gccatcattg
    14401 aaagtttaga tggcggaaag cctattcgcg aaagcagaga tattgatgtt cctttagcag
    14461 cagcccactt tttttattat gccggatggg ctgataaact tgattatgca ttgccgggaa
    14521 tggcagcacg acctttaggt gttgccggac aaattattcc atggaatttt ccgatattaa
    14581 tggcagcatg gaagattgca cctgcacttg cctgtggcaa tactgttgtc ctgaaacctg
    14641 ctgaaaccac tccgcttact gcattaaaac tagcagagat tttcagagat gcagacctgc
    14701 ctccgggtgt agtgaatatt gttacgggag ccggtgcaac aggtgcatcc attgttaatc
    14761 atcccggaat tgataaggtt gcctttacag gttctactga tgttggaaaa attattcaga
    14821 aagctttagc gggaacaaca aaaaagtaca cacttgagtt gggaggcaaa gcagctaaca
    14881 tcatttttga agatgcagcc attgatcagg ccattgaagg aattgttaac ggcatatttt
    14941 ttaatcaggg acatgtttgt tgtgcaggct cacgattgtt tgttcaggaa agtgttgcca
    15001 acgaagttgt acgcaagctc aaagaccgta tggaaacgct catcattggt gacccattag
    15061 ataaaaatac cgatatagga gccatcaact cacgcgagca attaaataag ataaaagcat
    15121 tggttaaagt tggggttaat gaaggtggcg attgctatca gtcatcatgc acattacctt
    15181 caaaaggttt ttggtttaag cctacaatat ttacaggtgt agcacaatca agtagaattg
    15241 ttcaggaaga aatttttggt cctgtccttt caattcaaac tttccgtaca gtagaagaag
    15301 ctgttgaaaa agctaataat acaccttatg gattaagtgg aggtgtgtgg tctgataaag
    15361 gtagcaaggt atttaaagtt acttcaaaaa ttaaagccgg agtgttatgg gccaatactt
    15421 ataataagtt tgatcctact tcaccatttg gaggatttaa agaaagtggc atgggccgcg
    15481 aaggcggtat gcatggacta agtgcttatt taaaataatt ttagcgtaat tacattatgg
    15541 agacgaagca aaaagaaaaa ataaaaaaag tagctgttgg acttaatggt cacagccatc
    15601 atgcagaaac cgaagtaagg ttagctgtac aaaaaacata taagctttac attgatggta
    15661 aatttccacg aactgagagt ggaagatatt ataagcttaa tgacaagaac ggaaaggtaa
    15721 ttgctaatat ttgtcgtgca tcaagaaaag atttcagaga tgcagttgtt gttgcacgaa
    15781 aagcacaagg cgaatggcag aaaaaatcgg catttaacag aagtcagata ttatatcgca
    15841 ttgctgaaac attagaaaca cgcagacaac aatttgtgtc cactttaggt cttcaaggtg
    15901 tgagtgatgc agcagcgcaa aaagaagtga gtgctgcagt agatatgctt gtttattatg
    15961 caggctggtg tgataaatac caacaggttt tttcatgtgt gaatcctgtg gaatcatcac
    16021 attttaattt ttcttttcct gagccaacag gtgttgtttc agctattgca tcacaacagc
    16081 tgggtttact gaatctggtt tctttaatgg caccggcact tgcaggaggc aattcaataa
    16141 ttattctggc agcagaaaag tttccactca ctgcaataga ttttgcagag gtacttaatg
    16201 caagtgatgt gcccggtggt gtcgttaata ttcttacagg ttttagtaat gagttgcata
    16261 cacacttttc atcacacatg gatgttaatg ccattgttta tgcagacaat aacagcgcag
    16321 cattaaaaac aattcagacc aatgcagcaa gtaatgttaa acgagtagtg gtagttcagc
    16381 atgttgccga agaaagccca tatcgtatca tggaacttca ggaagtaaaa actacctggc
    16441 atcctgtggg aatttaagat taagtctatc aatgtttttt gttttcatga atgtaatttc
    16501 tctaacctga gcgtaagtca aataaattgt tcatgaatga aaggattgaa taatatttgg
    16561 aaagattgaa atcatttgct gtcttcttac agaaatgatt tgtttgtatt tattttgatt
    16621 ggtagttatt attttaaaag acatattatc tttgtttttg ctttacaaca cctaacaatc
    16681 taaattaaat tttatgaaag tcattgctgc agatgacctg agtgcaacat cgctttccga
    16741 tagttcgcga cttcgaaata ttatttttat tttattgtat ttctcacaag gcattcctga
    16801 agggctaaca ctttttgcta tccctgcatg gcttgctttc aatgggaaaa gtgctgttga
    16861 aattgcagca tacagtgcaa ctgttatgat tccgtttagt ctgaaaatat tgcttgcacc
    16921 aataatagaa aaatacacct atctgcctat ggggcgaaga agaccttggt tgttgttcgg
    16981 tcagtttgga atcttatctg gattattcgc acttgctttt gtaccggagc cgctggataa
    17041 tattttcgtt atagtttgtg tagttctgtg tttgcacata tttgttatgt ttcaggatat
    17101 tgctacagat agtttagtga tagacattgt tcctgttgaa cagcagggaa gagccaacag
    17161 tttgatgtgg ggctccaaaa caattggttc atctgtgtca ctgtttttag gaagttggct
    17221 catcaatggg tatggtttta caagtgctgt tttaatttta tcagctccaa ttttagttgt
    17281 aatgtttgtg ccgcttttat taagggagca caaaggcgag aaacttctcc catggactca
    17341 aggtcaaact tctgatgaga ccaaacaatt tgtagttgaa agctggaaga aaatgtttaa
    17401 atcattcaag caagtagttt tactacgtaa ttccatctta ctgttattag cagtttttat
    17461 agcacttggt gcaatatatt ttataaatac tttactgccg atttttacaa tacaggaact
    17521 tggctggacc aatgtgtatt tctcaaaaat attttcttcg tgcagattaa taggaggtgt
    17581 tgccggaatg ttggttggcg cgcttgttat tcaacgaatg ggtgtgattc gatttattca
    17641 aagcagttta atattaatga ttgtaatagc tgttcttttt gttatgggtg ctgtattttg
    17701 gcatcacttg aacatcatag ttggttttat tgttactttc tgcatattgg caacactgat
    17761 taatattggt gtgcttgcct tagccatgca attatgctgg aaaagagttt ctgcaattca
    17821 atttacattt tgtatgacaa tatttaatgc cgggttagca acaggtgcag cattactggg
    17881 atggcttcgg ccttatatgg agtggcagca gctatttttg gtttttgccg gtttgatagc
    17941 atttgcaatg gtgctgttta aatttatgaa aacaacaaac catgtaaaac aagttgaaag
    18001 gttagaagag cagtatatgg atgttttaaa agtagaagga agtttactgg taaaatcaga
    18061 gacaatttaa ttcgaaaaat aaatttcgta atttaaaaaa taccttaagg caacaaaatc
    18121 cccggaaacg aatttgcatc tgtttgagga atgcgtgttt gatatttttc gttaatagct
    18181 tttataaatt cattggcaat aagtgcactg cctcgccctg taggagtaat gccgtcaaga
    18241 gaaaaaaatt gccctttaag aaactcactg ctgtaggtaa caccattgtg cggataacca
    18301 ctgcttatct ttttaaagaa agcatacatg tccacatagg ctaaattgtt ttcttcagct
    18361 tttgttttta gataattgtt gtagcttgta attgtatttc tcaatgtatt gatttctgaa
    18421 gtagtgagta caaatctgtt gcctaatggc ttttgtgtat tgctacccca gcctgcgcag
    18481 tttatactat caagtgtaac cggcaacagc actaactcac ccggcttcat ttgtcttctt
    18541 ccggatgctg ccgaagggtc agaaattacc aatggattgt tacccgcttg aaaagttatc
    18601 ggtggattgt ttgaagcata cagtgtatta agatcctgtg cctgttgctc tgtaagtacc
    18661 agagcatcgt aggcaataga agtaaaataa ggaagattga gtaggtccgg aatatttgcc
    18721 actacacctt tgcgatttcc tgcaatgaga gaggtaagtg cattgttgat attattttga
    18781 aaattagcta atggtgtaat ggtagccaat gtttgattgc tgcctgccat tgcacaatca
    18841 taaatatcgt taaagccaac ccaaaaagaa aagaaagtag gcaattgtct gactgcatcg
    18901 gttagtacgg ttgaagtttc ggggtttgta gcaaatcgcg aatagaaagg attcttggaa
    18961 agcagtaagt ctgttgaacc aaaattggca ttgtccaaat cagatgattt catgcccggc
    19021 acacccatgt tgttgtatgg agcattgttg ccaatatatg tttcaactgc gacaatgtca
    19081 gttacgttgg aaggaaatgt aaagaagccc ggagtggcat tgcatgttgt gccatagtta
    19141 agttttgttt gtggaatata ggtctgaaga aaaaagttaa agcctgcacc atcatttccg
    19201 ctaaccaatg gttgcaaaaa ctcacctcca ccaactttct tcaactgtcc tgcaagaata
    19261 gctgcaaaac tattttgttg ccccatgtca aacagtgcat tattctgata gcctgcggtg
    19321 taagaactgc ctatagcaac aaacttcgaa aagtcaatgc tgttgttgtc gaactttagt
    19381 tctttcaact caggtttgca tcctgcaatc aatattactg caagaagtaa acttttcaga
    19441 aggtgattat tactcatgtt tttttgttgt ggtttattta attaaaatga atagttgaca
    19501 ccaacaccaa aacaatgcga caatgtattg aatgcaccat ttaaaccggt atctttattt
    19561 attccttcac gctcagcctt agtttcaaaa ctgtatgatc cttcaataac aaaagttttg
    19621 ctcacgttaa acgatgcacc aataccaaac acatatctgt cgccatcggg catgtcagga
    19681 gtcatgtagt tttctttaac cggagtctga tcgaacgcaa tacctgcgcg gcctgtaact
    19741 ttatcactga ttttatacat cgcacctaat cgtagtgata atgcatctct gtattttcgt
    19801 gcctgacgaa attcatcatt caaagttgtg tttggtgcat caaattcata gttcaaagaa
    19861 tcgaatgatg accagccgtt gaggttggtt tcaaaattta gttttaaccc tctaatgact
    19921 tcatagctga acccgataga caaaacagaa ggtaacggca tttctgcagt ggcatttgct
    19981 gaggccggat attggttggc taatgaagca ggaatattgc taaaggaaac atcagtgttt
    20041 ttaaaattga gtttaacttt tgatcggtag gtaagtgcag cggttaattt tttattaatg
    20101 gcataattga tacctgcatt aaaaccgaag cctgttgcct taccgtcaaa ttgtgcatct
    20161 gcaccatcag caactatcaa tgcacgattc catttatagt tggcactggc aaaaacaaag
    20221 cctgcaccaa ttcctaattt ttcagaaatt ttatatgaga aagtgggttg aataaataag
    20281 gtgttcaact ttgctgacaa aacaatatat cgcccttccc aatcgctttt ccatgttgta
    20341 ttatatccaa aaggagtgtt gatcacaata cctgcagcca ttttttcaga taactttatg
    20401 gtgccaccaa gataaaaagg caacatgttt tttgattctg tacgatctac caatccactg
    20461 ttgttgcttc gaaaagcaat tcttgactgc gaataagaaa taccgccaaa gacattactt
    20521 ttctcaagaa aaaccattcc acccggattg taaaatatta ctgaaccatc agtttgcaat
    20581 gcaatcactg caccggccat accggctgaa ctatggcttt gtgatagcat ctgaaatcca
    20641 ccgcctgata catgaagatt acagaataga aacagaatta aaaaacctga aatgaatttt
    20701 ttcatattaa tcttaacgca aacaaaaata agaacagcaa gcttaataag caaagttata
    20761 catgacgatg acaatttgtt gttaacattg ctttgttggt catggttttt aaaaatgttt
    20821 aagatgtaac aaccaatatt caaaattttt gtattaaaat tgcactgtaa ttgtaacaca
    20881 ttttttcacc tgccaaaatc atttcacatg ttagtcaaaa agttatttct gatattattt
    20941 tctgttgcaa caactttaac ctgtgcggca tctgttggcg acactacatt tgtaagttca
    21001 cataccaatg acaccgtagt aaccaatcct aacactggaa gtaatggtta tgcaaattgg
    21061 gctgtatttc cttcaacgtc caccaactat cgtaagataa tcttgacttt aacgcaccgt
    21121 tgtccaagcc caatggcatg tggtgaatgg gattatctcg attacattta catcagacgc
    21181 acaggaagtc agaacaatcc ttctcaggat attgaattgg cgcgttatat tacgccttac
    21241 ggaaacactt tcaactccac atttaaatca tcgtggactc ttgatgtaac agattttgca
    21301 tccttccttc acgactctgt agagatagaa tatattcaca caggctacga aaccaatgtg
    21361 ggtcgcggat ggcttgttag tgttgacttt gctttgatag aaggtactcc ggtaatgaat
    21421 cctattgcat ttaaaagatt gtggcatggc agtttccctt atggtaatgc tactaatcct
    21481 atcgaaaact atctcggtgc cgacacggtg acacttaatt tcaacacagc aaaccttaga
    21541 ttgaaggtta cacaatccgg tcatggtgca gatgctaatt actgtgcaga gttttgctca
    21601 aaaacaagaa ccatttattt tgataattca ttggttgata caaagcaggt ttggcgtttg
    21661 tgtggaacaa atcctattta tccgcaagga ggtacatggg tttacgacag agccaactgg
    21721 tgtccgggta attgggtcta tccttctgtc aataattttg ccgttgcagg aggaagtaca
    21781 cacattgttg acatgaatat gcagaactac aacgtaacaa gtccttcggc taattacgtc
    21841 attgaaggac agctattcga atttggtcca ttgaacaata gtatagatgc agcaatagat
    21901 gaaatacttg cacctacaga caagtttgaa ttttcacgaa gcaaccctac ttgcaacaat
    21961 ccaaaaatca gaataaaaaa taatggctca acaccaataa catcagcaac aataaaatat
    22021 ggattagtag gcgaacccga aagtacattc aactggacag gcacaataaa tacttttgca
    22081 acaacagatg ttgacttacc tggattaatc agctatacaa gcggcaacag aatttttaaa
    22141 gcatatattg ctgatgttaa taatacagca gatcagtatg tttatgatga tacagcttat
    22201 tccaatgcct atgttccgga agtgatggac acagttttta tcctactggt aaaaacaaat
    22261 aattacccta ctgaaaatgg ctatacactt tatgatgaaa ataataacat catcgttcaa
    22321 cgacttgccg gaacattagc agcaaataca atctatcgcg acaccattca tgcaactccc
    22381 ggttgctatc gttatgagat tactgacagc ggtgatgatg gtttatactg gtgggcaaac
    22441 tcagcacaag gaacaggtta ccatcgtatt ctgcgattga acaatagcat tgcaagaaat
    22501 ttcgaaagag attttggttc acgttacagt taccgatttg tatcttctcc gggtgccatt
    22561 gtgggtatca acaatcagga gcgttatgct gatattgtag tttatccaaa tccaaccaag
    22621 ggtatcatca atgccgatat tacattaact tcaacaagtc cgttagaagt gtcggtatat
    22681 aatcctttgg ggcagcttgt ggctgaacag agccgaagta attttaattc cggtataatt
    22741 tcttttgatt tcaaaaatca acctaaggga atatatttag taaaagttgt tgctaacgat
    22801 caggttaaag tgcaacgagt aatactggag taaatagtaa tgaaatttaa ttttacagca
    22861 ggtagtttag ttaaataagt acctgctgta acttttagta acaggcctgc gtcatacaat
    22921 aaaatgaagg atatgaaaag aagttatcaa tttagtctga ccttgttttt gatgttgttt
    22981 gcactttttg caaatgcaca aaaacatata actttagagc agtttaccgc tattgactta
    23041 cgcggatcgc ttaatgcagt ttataaatta gacagtacat gggctattga agtaccggaa
    23101 gataatgtca ccacacagtg gaatcttgaa aataaaacac ttgtgttaaa cggtgcagga
    23161 agtaatattg ttgtcagaag tcctgatttg gagaagataa acatttccgg ttcgggaagt
    23221 ctttctgcag aagatactat ggaattgcac gatttgaata ttgatatatc aggaagtgga
    23281 aaggttaaga tgaactacgt aaaggcaaag aacattgaag ctaacatgcg tggttcagga
    23341 aaaataattc ttggaggttc tgcacaatca ttaaatgcaa atcttgcagg ctcaggaaaa
    23401 attattactc ataagttaaa tgttgatcgc gcagaagtaa atgttagcgg tagcggcaca
    23461 gcttcggttt gggttagcga ttctttaact gctaacattg caggctcagg taatctgtat
    23521 tatgtttctt ctccaagaac aattaatcag gctattgctg gctcaggaaa aattgaaccc
    23581 ataacaccgg caattgaatc ggagttgaat tcaaacagta tttcaattac cacaagtaat
    23641 aaatcaggta cctactggtc tggatttgag ttgggattta acggtcttta taacagcaaa
    23701 tcaaaatttg acactcctga tggatatgat tttttagaaa tgattcccga aaaatcattg
    23761 gctgtaaata tcaactttta cgaatacgag gtaaaactga taaaacgata tgtgctggct
    23821 tctaccggaa taggcctttc atacaacaat taccgattca gaaaaaatat gagcctgatt
    23881 cctgatacca atgcattgct ttatactctg gacaccatga atctgcgtaa gaataaactt
    23941 actgtaagtt atgctactgt tcctgttctg ctgactttta attccagttc aaatcttaaa
    24001 aaagcttttc acattaccac cggtttgttg tttagttaca aacttggttc acacaccaaa
    24061 aaggtttaca ataaagatga taaaaaggag aaggataaaa cttttgacga ttttaatatt
    24121 gatcccttcc gctatgatgc tactatcaga atcggttata gcgattttac agtatttgca
    24181 aactatgcac tgtctgaact ttttaagagt ggtaagggtc cggagttgca tccctggaca
    24241 attggagttt cattgctgcc atggtaatag aaactacctt aacggcatta gccaattgtt
    24301 attaagaaaa tcaaattaat ttctgtgtag gtgtgaatta tttcacacct acatagttta
    24361 tgacatcatc cttggtgatg gttttgcctc cgagaattac caaacgttct accacattac
    24421 gcagttcacg aatatttcct gtccagtttt ttttctgtaa ctgctctatt gcatcaggag
    24481 caatttcttt tgcagggctt ttataatcat ctgcaatttc tttcaaaaaa taatttgtca
    24541 acacaggaat atcttcctta cggtcatcga gtgatggcac atgaatcaga ataacactca
    24601 ggcggtggta taaatcttct ctgaaattat ttttttctat ttccgatttt aaatctttgt
    24661 tggtagcagc aataatacgt acatcaacag gaaaatcttt ttcgccacct actcttgtaa
    24721 ttctgttttc ctgcaaagct ctcaatactt tggcttgtgc tgacagactc atatcaccaa
    24781 tttcatcaag aaaaatggtg ccaccgttgg cttgttcaaa tttaccactg cgtgttttta
    24841 tggcagaagt aaatgcccct ttttcatgac caaacaattc gctttctata agttctgatg
    24901 gtattgctgc acaattaact tctatcaatg gcatgctgtt tctgctgctc ttttcatgaa
    24961 tccatcgtgc caccaattct tttcctgttc cattgcttcc tgtaatcatt acacgagcat
    25021 cagtaggtgc cacacggtca atcatgtctt ttatcttacg aatgccttca ctttcaccaa
    25081 tcatttcatt ggtgcgtgac atttttcttt tcagcgtctt ggtttctttt accaaaactg
    25141 tacggtcgag tgcattacga acggtaataa gaatacgatt gaggtcaagc ggttttgcta
    25201 taaaatcaaa tgcacctttg cgaatggctt caacagcagt atcaatgtta ccatgtcccg
    25261 atatcatcac cacaggcact tcagggaatt tactctgtaa gttctccagt acttcaatgc
    25321 cgtccatgcg cggcattttt atatcacaaa aaataatatc gtatttttct ttctcagcca
    25381 tcttaatacc ctcaatacca tcgggcgctt catccacttt gtaattctca tattccagaa
    25441 tctctttcag cgaatggcgg atgcttttct catcatcaat aattaatatg cgtgacatac
    25501 tcttttgttt acggtcagtt tactgttgtg attgtttatt cattgcaaaa taaagcattc
    25561 cttcctttaa tgcataagca ctcactaaaa gtttattcat attgtgtttg tgaataacat
    25621 agtgtagcaa taaggatgca acaacaatca tatcggcacg catggcaatc atccccggca
    25681 tttcaagtcg ctgtgctacg gttgaggtta acaaaatttg atgtacatca aaaaattctt
    25741 caagttccat tgaccaccag gttgtgtttg tcttagctct gtgaaaacga tgaagacata
    25801 aatcaagaaa ggtatcgaaa gagcctgctg agcctaccaa tgtatttaca ttgccatgtt
    25861 gttttaatgc ttcggcaagt ggtaataaaa cggagttgat atgttccgtt attaatttca
    25921 cctgcacatc atgaatcggg tctgatggtt taaacatatc taacaacaag gcagctccca
    25981 atggaaaact ttgccgccac aatatgtttc catttccggc aatgacgaat tctgtgctgc
    26041 cgccaccaat atccataatc agcacttttt cttttccaaa tggtacagca tgactaacac
    26101 ctgagtaaat caattctgct tcgcgcaagc catctattat ttcaagtgta atgcctgatt
    26161 gtttttttgc atcttcaaca aagtcgtttc catttgttgc tctgcgtatg gcttctgttg
    26221 cataacacaa ctgtttgtct atgacaaatt gtttcatgtc ggcagcaaat tctttaagtg
    26281 catcaatgcc tcgattgaag gcttcacgcg ctattttatt actgctgatg ccgccttctc
    26341 caagttttac agctaaattt ttgcggaaaa gtattttaaa actatcatct gaaatgtcgg
    26401 caataagcag atgaaatgtg ttggtgccac aatcaattac agcaatggcc attgatatta
    26461 tttataaaac aaccaacgaa acaattcttt gtaggtaggc tttttgccat acatcaatat
    26521 tcctgtacgg taaatttttg cagctatcca cgtagtgaaa ataaaccctg ccactaacaa
    26581 taccatcgaa atagcaatgt caattaaagg tggatcaaag ggcatgcgta ccatcatcac
    26641 tatgggtgat gtaaacggaa ttatcgaaaa ccaatagccc agctcacttt ccggattggt
    26701 catcacaatt gtagaaacta cataggcaaa aatgagagga atggtaatgg gcaacataaa
    26761 ctgttgtgtg tcggtttctg catcaacagc agcaccaatt gcagcaaaca atgcactgta
    26821 aagcaaatac ccgcctaaga aatagaatac aaaacacaat aaaatttttg tgatgttaaa
    26881 tccttcaaat attttcataa aatccatgct ggcgggttgc atcgtttctt ttggaatatt
    26941 gctgctctga gtcatttgtt tttgttgcat ctccatgagt ttactctgat ctttacctac
    27001 tatcaatgat gtggcagtgc ctgaaattgc cattgtaagt attatccaaa gcaggaactg
    27061 cgtcaatcca accaatgcaa tgcctataat ttttcccatc atcaactgaa atggttttac
    27121 tgatgatatg ataacttcaa caatacggct ggttttttct tcaataacac cgcgcataac
    27181 ctgtatgccg tacatgaaaa taaacatata aattagcagt cctgcaccaa agccaatgcc
    27241 tgctgttact cctgcagaaa atgatttgtc ttttccactc gggtcaacag gtttttgttg
    27301 aagactgatt tctgttttta tggaatcgag tgtagatttt tgaatgcctg catctgtcag
    27361 cttcatattt tcaatgttct tactcagctc actttccaaa taacccacta ctgtaagatt
    27421 aggttgcttc tctgtataaa gcactgtggc atcaggcttc tgcaaggtgt tggcaggtaa
    27481 ataaagtatg ccataatctg tgtttggatt aaataaatgc cttgctgttt gaataggcat
    27541 ggtgtcttta gtaaagatta cagttttaga attggtgagt ttaccagcaa gaactcctgt
    27601 ttcgtccact acacgaatca tcgaaataga atcaccctga ttcatggaaa ggtatgcaaa
    27661 tcctgcaatg agtccaccca taagcaatgg acctaatatg gtcattatga taaaagactt
    27721 tttctttacg cgtgtcagat attcgcgttg tataatcagc agtatgatgt tcatgcttct
    27781 aaaggtttgt tattttgtac cgattcaata aaaatatcat tcatagtggg tatgcgctca
    27841 cggaatgaaa ttacttccac atcgttgagc agttgtcgca acaaatcgtt tgcactgctg
    27901 ttgttcagat tttttactat tgcaaatgca tggtcgtctt gtgttcttgt ttcaaccaat
    27961 tcaaaacctg tccatagtgc attggcaaaa cctacagacg tacctttgta ttcaatctca
    28021 aaaagattag aacggaattg tttccgaaca tcctttacct gcccatccag aatttttttc
    28081 gatttattaa tcagtgcaat gtgtgtgcat aattcttcta cactctccat acgatgtgtt
    28141 gacaggataa tggtagtacc tttctgccgc agtcccagca attcgtcttt aatcaggtta
    28201 gcattgattg gatcaaaacc ggtaaatggt tcatcaagaa tcaacaggcg cggttcatgt
    28261 agaatagtta ctataaactg tactttttgc tgcatacctt tgctgagttc ttcaactttt
    28321 ttgcgccacc aactttgcat ttcaaatttt tcaaaccaat aacgcaactt ttttaaagca
    28381 tctgcacggc tcaaaccttt tagtcgggca aggtaaatag cctgctcgcc cacttccatt
    28441 tttttataca aacctctttc ttcgggtaaa taaccaattg cagaaatgtg agaaggattc
    28501 agcgtttgtc cgccaaataa aatactaccg ccatcaggcc ctgttatctg attgatgatg
    28561 cgaatgagtg aggtttttcc tgcaccgttc ggacctaaaa gtccaaacac actcgactcg
    28621 ggaatttcta tactcaccga atctaatgct ttgtggttgg cataaatttt tgaaacctct
    28681 cgtatggaaa gcagattcat agttcaatat tatgcgacaa atgtaatgtt taagtaatgt
    28741 gataaaaatg aattccatac ttcgttttaa acagcgctgc atattgtctc ataaaaatta
    28801 tttctatctt tccacctttc ataaatttca acaatgcggt tgctttcttt tgttttatgt
    28861 ttcatgtttt tgtctgtttc cgtttgggca cagcaagaag agttggattc attacaacag
    28921 ttgcttcaca aacaagctga cgatacatct aaagtaaata cccttaacga actcagttat
    28981 gtatattatc agactgctgt ttatgacagc gaattgtatt atgcacaaca ggcattaaac
    29041 ctatcgcaaa agttaaattg gaaaagaggg atggctgttt cattaaaaaa tattggcagt
    29101 gcctgcgatg atatgggtaa taacagcgat gccttatatt attacggacg tgctttaaat
    29161 attttcagtt ctattgatga caaatccggt gtttctgcat gttataacaa tatgggcttg
    29221 gtatatcgca caattggtaa tttttctgaa gctttaaaaa atcattatgc atcgttaaag
    29281 attaaagaag caataggtga tagaaatggt attgctcttt cttataacaa cattggagtg
    29341 gttcttaatg ctcaaaaaaa ttatgccgag gcaattaaaa accatcttgc tgcattggag
    29401 attagaaaag cattaggcaa caagagaggt atagcttcat cttacggaaa catgggaaat
    29461 gtttatcagg ctcagggaaa ctatgcagaa gcattaaaga attatgagca gactttaaaa
    29521 attgaggagg agattggcaa taaagtagga atgacaacag ccctcaacaa tatgggtgca
    29581 gcctattatg aattgggcga ctacgagcag gcattacaac acaatactga cgcattgcgc
    29641 attgccgaag aagcaggcga cctttcatca attgcagcat caagcatcaa caccggcaat
    29701 gttctggtaa agctaaaaca accacaggca gcatcggaat ggcttcaaaa ggctctggaa
    29761 ataggaaaag aaattggtga agttgaagtt ataaaaaaca gttacgaagg actcagtcag
    29821 gcagacagtg ctttgggaaa atacaacgat gcattgcagc attacaaaat gtttatcagc
    29881 tatcgcgaca gcctcatcaa cgaagagaat acgctaaaaa ccgttcagtt gcagatgcaa
    29941 tatgagtacg ataaaaaaga tgccatagcc cgtgctgttc aggagaaaaa agatgaagtt
    30001 accagacagg aagtacaaaa acaaaaactg cttcgcaaca gttttattgc cggatttgca
    30061 ttaatgcttg ctcttgctgc cgttagctac agaagctatg tgcgcaaaag agaagataac
    30121 ctgctcatcg aaaaacaaaa attattggta gaagaaaaaa acaaggagat tacacaaagc
    30181 ctcaattatg ctcgccacat tcaggctgct gttttaccca atcacagcaa catcaatgcg
    30241 ctgcttaaaa actattttat tctgtacctg cctaaagata ttgtaagcgg agatttttac
    30301 agtgtgttgc aaaacaacgg cagggtaata gttgctgctg ccgattgtac cggacatggt
    30361 gtggcaggag cttttatgag tatgattggc acctcgctaa tcaatcagct ggtaaatgaa
    30421 aaaaatataa ctacacctgc tgtaatctta gatgagctga acgaaggcat tatcaatgca
    30481 ctgaaacaga gacaaggttt ttctaatgat ggcatggaca tagcactgat ttgtttagac
    30541 catcaaaaaa agcaattaca gtttgccggt gccaaccgcc cgttgtggtt gtttcgcaac
    30601 catgaactgc tggtggtgaa acccgataaa tatcctattg gtggtatgca ggtagcacac
    30661 accacccaat ttaccaatca tgtgatagaa ctgcaaaccg gtgacagttg ctaccttttt
    30721 tctgacggtt ttgccgatca gtttggaggc gaacagggta agaaaatgat gagtaaaaag
    30781 tttaagcagt acctgcaggt tatgcagcac ctgcctatgg acaagcagaa agaacaactt
    30841 gagcaacttt ttaattcatg gaaaggaaac tacgcccagg ttgacgatgt tttggttgtg
    30901 ggcataaaga tataatagcc ggcaaatgtt aaaaactaaa tttttcaatt agcgttgttg
    30961 ttttattttt gcatcgttgc caatggcaac aaagaacctt tttgtaaatc attaaaacca
    31021 aatattatgt ttaaaaattt attcttttcg agaagactaa acattgccgc tttatttctg
    31081 gcagcgtttc tctttaatct gcaaagtagt gttgggcaga cttctactca aaactttgga
    31141 acgacaactg gaagtcatac atctggtacc ggttcatcga gttttattcc taacccaact
    31201 tcaggtacga cctatgctag gggcggagca actgcgccta atgcacctgt taatttggaa
    31261 aacacatcca accctttagg aaccacaggt tcctttgtca gagcagttgc ttcatcttct
    31321 acttcagtta ctaaagtttc acctgcaatt gcttatacag caggaaaaga gttttatact
    31381 tcgatgaagg ttctttttgg agatgcctct gcaagtaata ctgcaaatag tggagagtgg
    31441 gctttttttc aaggtgatgg tgcaatgtat aacaataaca gtggatttac aggcaatcaa
    31501 gtttttactg gtttacgatt tacttatgct tcaggtgtag tcaacttgga ttatagaagt
    31561 ggaggcagtt ggtcaacatc tggacttctg actacttctt ttaatcaggg tactacttac
    31621 attattgaaa ttgttggaaa taacaagtca agtggaacta ttaattatac ttacaatgga
    31681 aacccaaagt cagttgctat agataaattt gatttatata ttaatggtac agaagtaggt
    31741 gatgatttgt ctaaagcaca attaactaat aatacagaca ttaagagtac aacctttact
    31801 gggcaatcaa gtgcatcgaa tgttgctaat ttatttctag atgatgttgt tatttataac
    31861 tcagtaccag ctgtgattgg ttccggaagt tcacctattt tatccgtagg ctcactttcc
    31921 aatcaattcg gtaatgtttg taccggcaca acaacagcct atgaaacatt taccgtttcg
    31981 ggagcaaacc ttgatggcag cagtgtggat gtagggacat taagtggttt ttcttattgc
    32041 cttactccta atggtaccta tacttcaacc ttaaaccttc cttatacagc acctacttta
    32101 aacagcacta ctgtgtatgt aaaattcagc cccacgttag tacaaagcta cactggcacc
    32161 attccggtta ccggaggtag tgctgcggca tcaggttgtt ctgtaacttc tgctgcgggt
    32221 attaacacca tgccatcgct tactacaggc gcagcaacgg gtgtaacaca aaatacagcc
    32281 acctgcgaag gaaccattac cgacaatggc tgtactgctg tttcggccta tggtatagaa
    32341 tacagcacta ccaacaactt tacaccaggc accggtacac aggtgagcag cagtaacatc
    32401 agcagcaaca cctatacttc tgcccttagc ggattggcat caagcacaac ttactactac
    32461 cgtgcctatg ccaccaacac tgccggaaca ggctatggcg ctcaggcctc ttttaccacc
    32521 gatgccatca atgcaccggt tgcagttgct gctaccaaca ttggcataca tagtttccgt
    32581 gccaactgga atactgtgag cggagcaaca ggatactttt tagatgtttc tacagcatca
    32641 acatttatgg ctcccataac tactgatttg gtttattggg atttccccaa tagccctgat
    32701 gatgctactg ccgatggtgg aatcttggcc aatgtctcgg ctaccatctc aacacaaggt
    32761 ggaaccagtg caatcacata ttcagctgga gcaactactg aagctgcatc agctacagga
    32821 tgggatggag gtaatggcac aaaatattgg gaaataaatt ttgatgctac caactactac
    32881 agtctggcat tttcaagcaa acaacgctct tcaggtacag gtcctcgtga ttttaaagta
    32941 cagtataaaa ttggcagcgg tggcacctgg acagatgtaa caggcggtgc tgttacagta
    33001 ggtaatgatt ttacaacagg ggtattaaca gatattgcat tgccaacagc ttgtgataac
    33061 caaagcagtg tttacctgcg ttggataatg accagcaaca cttcagtagg caatggcact
    33121 gttgctagca cgggtaccag ccgtatggac gatttgtttg taaccggcta tgaaggtgat
    33181 tttgttgtaa acaaccaaag cgaagctgga ttattttatt atgttacatt gctcagtcct
    33241 tctaccacat attattatcg tgttcgtgcc acagatggcg tgctcacctc tgacaacagc
    33301 aatgttatta acctaaccac attaggcgtt tgtgatttgt ttacagtaaa tgcatcgagt
    33361 gatgcaccat cgccaatatg ctccactacc aacatcaact taacggcaac tgccagcggc
    33421 tctgatggaa ctattacagg ctacgaatgg tcaggcccgg gtggttatag caacatgaca
    33481 caaaacccaa cagtagtaaa tccggttagt ggaacttata cagttaccgt aacagatgac
    33541 aatggctgta ccgttaccga taatgtatcg gcaacagtag aaactgcacc aacagccaat
    33601 gccggcagcg atgatattat ttgctcaaac ggaagtgctt cactaaacgg ttctatcggt
    33661 ggcgatgcta ccggtggcaa ttggacaaca accggtgacg gaactttttc gcccaatgct
    33721 acaacattaa atgcatctta tattccgggt acaacagata tcagcaatgg tagcgtagtg
    33781 cttacactta cctctacagg tagtgtattg tgcagtgcag ataacgacca ggtgacaatt
    33841 accattcaaa caggtgtgcc ttcgcaacct gctgttgtaa caggagcgcc aaccagcgtt
    33901 tgccctcctg caggccctta tacattaaca acctcggctg caaacgccaa cacctacaat
    33961 tggtatgtag ttggtgcaaa taccggagta acatttttat ctgccaatgg cagcaactca
    34021 atggatgtac aatacggtgt tactgctaac agcagttatt ctgtacgtgt agaagccagc
    34081 aatgcctgcg gtaccagcgc ctatcgttcg gcctttacac gcagatctgt ttcaacacct
    34141 gcaagtgtta gtggaaacaa agtggcttgt gccagcacca gcgacacata ctcttgcact
    34201 gcagtagccg gagcagaatc ctatcagtgg tctatcacag gtgatgctac cgttagcgga
    34261 actaccaaca gcgttactgt aaactttggt cctacctgga caagcggtac gctttgtgtt
    34321 tcatctaagg taggttgttt cactagtccg gcaaaatgtt ttgtcatcac ttcttcaact
    34381 ccttcagtag gtaccattac aggaaacact actgcttgtc ctaatggtgt agtgtcattc
    34441 agtgttgctc cgcttgcagg cattcaaacc tacaactgga caactcctgc aaactcatca
    34501 gtaactttag gagcaggtac aaacaatgtt caggtaactt tcaatggaag ctacaatggt
    34561 gttggaaata tttgtgtttc tgctaccagc atttgtggtg taacaacagc acctaagtgt
    34621 aaaactgttg ctccgggtct tccttcagtg cctgcttcaa tcacaggtgg aaccaatggt
    34681 ttgtgtaatc aggcaggaag cttcagttgt ccattgcaag ccggaaattc ttataactgg
    34741 actgcaccag gtacaatcaa taataatggt tcaaacagtg ttagtattca gttcagcaat
    34801 ataactacag gaagcgtttg cgtttctgca tccaacagtt gcggtagcag tgcctatcgt
    34861 tgtgtaactg ttaaaggtgc tcccaactct ccaacaggac ttacaagcaa tcctgctgtt
    34921 atctgtgcca acacttccgg tgttgagttc aatgccaaca cttctaacac tacaggtgcc
    34981 tacacattga catggaccta tactccgaca gcggcaacct acaatagtgg tggtggaaac
    35041 acaaaccaac tgattctcga ctggggaaat accaacggtg ttgttgtggt tacagcacac
    35101 aatgcctgcg gcagcggtgc taaatcgtat gctgtgacgt tagactgtcg tatggaggga
    35161 gaagcatcaa atgctataaa gatgtaccct aatccggcca gcagtagtgt aaatgttgaa
    35221 tttaattcag ataaaaacga aacagtaaaa ctcatcatca ctgacctgag cggaaaagta
    35281 gtgatgcagc aatcgttaaa tgtcactgta ggcaacaata cattacaggc agatatttct
    35341 caactggcta aaggtattta catggcgcaa ctttcaggca acaatttgat gtcgccacag
    35401 aagctgattg tagaataatt ttctcgtaat ttaattgtga gagaaaacga gccgttgtgc
    35461 gaaagcgcag cggctcattt tcatttaggc cggcagtatt gtgttacatt atattccaaa
    35521 aagcaaacac taaaagtaca ccatctacac aaaaataccg gtgtcgctga agtccgttgg
    35581 attgtatcgg gatgtttaca taacactaaa cccatctatc tgtgaaccgc taagtacgcg
    35641 acccgtacgc ttagtggtgt gagataccga tagctatcgg tagcactccg tcagtctc
//
LOCUS       DAOQNP010000022        35652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_384_length_35652_cov_9.007276, whole genome shotgun sequence.
ACCESSION   DAOQNP010000022 DAOQNP010000000
VERSION     DAOQNP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 35652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35652
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_35652_cov_9.007276"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKY09_04790"
     CDS             <1..222
                     /locus_tag="PKY09_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNB12206.1"
                     /translation="ESYFFKKPCVILRNETEWVELVDAGTAIIAGADSEKIIAAYQSL
                     KTASLNFPPIFGDGKAAEFICNEILAAFN"
     gene            249..953
                     /locus_tag="PKY09_04795"
     CDS             249..953
                     /locus_tag="PKY09_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12207.1"
                     /translation="MKHILSKSSYIRSLQCLKSLYFYKFHYKDRDPISAEQQAKFDRG
                     HQYGKITHQLFPGGIDVSPPTPFEYATSAKQTQALLLKQQPVIYEAAFIANEVVVALD
                     ILVRKGGLLHAFEVKSTTAIHPAILNDAALQYFVITNAGYGLEDFSIIHLRPDYKDLP
                     ADDIGALFVTESVKEYCVSQQQFVEDNILSAKEIIRNKKMPVKEQGEHCNSPYPCDFK
                     NICNKSNSYASGSLFG"
     gene            complement(955..3549)
                     /locus_tag="PKY09_04800"
     CDS             complement(955..3549)
                     /locus_tag="PKY09_04800"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNB12208.1"
                     /translation="MKKSLLFFAWVSLCLLSNQLDAQQWIVQPSNINPNNYVQFIDAV
                     DTNVVWGLASDRTSQQNPVQEFTRTTNGGQTWTAGTITNAANHGPSCIMALNADTAWV
                     AMFNVSGGGSILRTNDGGVSWTKQTTATFTAPNGFPNVVHFFNANEGFCMGDPNGGYF
                     EIYTTADGGNNWIRVPQANIAANLTGEFGITDVYTNFGDSTVYFGTNKGRIYKSADRG
                     ANWTVSSTPYTGYIGAITFRDANFGIACEADNATTSTDLIITNDGGATWNLLPTSSAG
                     FTMKQSIRFVPGTDSTFVITSPYTIFGSAFSVDNGSNWRLMDNLIHSDCDFVNATTGW
                     SGGGELNSPIYKWTGPMTIAANDATVKSIDVRSAVGTGTQTPMATVLNNGLTTQTFNV
                     TMDISGGYTSTKTVSNLAFNQTQQISFDPWTPGATGSYNITVYSQLATDSDLSNDTLN
                     KSTTAFNELPNYGWTSKTSAAIATFGGASAFIPSVNAPLDSGYLFSDGGSDLSIIQPK
                     NERFDEMTNNWTSATDMPIGVYQFSMHGIQNMMYAIGGYSSGFVPSPTNYIYNPQTNS
                     WTSGAVMPQPVGDYASGIYNDSLIYFIGGYDGTNDLNTVQIYNPATDSWSMGTPKPGT
                     AVSGFRGGIYNNFIVVTCGYSQTLQDAIDETWIGQIDPANPTSIVWQAAAPYPGGTMT
                     RFAGGGTFKNSLPYVVFAAGDPTGVGTEVKDDVWAYDLNNNEWLVGPPKPTACSNVSN
                     FTGVVSNDSLYMVVVGGYNGVGTTGANEWLNLGASPFVGVKELSSQLVVAVSPNPVSD
                     ILTVQIPSPLEMTTLAVFDLNERVVKEIQANSNKVQIVVRDLDNGVYILKMSDKKNSS
                     TARFTVMH"
     gene            complement(3686..5884)
                     /gene="pnp"
                     /locus_tag="PKY09_04805"
     CDS             complement(3686..5884)
                     /gene="pnp"
                     /locus_tag="PKY09_04805"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNB12209.1"
                     /translation="MNLGVKHTFTLPDGREISIETGKLAKQADGSVVVRMGDCMLLAT
                     ACAKQDAMPGVDFMPLTVDYQEKYAGVGRFPGGFFKREARPSEYEILISRLIDRALRP
                     MFPENFHADTQVLVTLHSGDSNIYPDALAALAASAALTISDIPFNGPISEVRVAQIDG
                     AMVINPYISDIARATLDLIVAGSMENIVMVEGEMSEVSEATILEAIKEAHEAIKLQCK
                     AQLDLLEKIGGKTKREYNHEEHDEDLRKLVRDETYQQVYQVALQGNPNKNERKKSFEA
                     IVEAFKTRFTEEELEEKSAFINHYYHEVEYDAVREALLAEKKRLDGRGLTDVRPIWSE
                     TNYLPTAHGSAVFTRGETQSLTTVTLGGKMEEQIIDGAMIEGTKKFLLHYNFPPFSTG
                     EAKPMRGTGRREVGHGNLALRALTKALPPESENPYTIRVVSDILESNGSSSMATVCAG
                     SMALMDAGIKMKSTVAGIAMGLISDGKGRYAILSDILGDEDHLGDMDFKVAGTRNGIT
                     ATQMDLKVTGLPYEVMAQALEQARQGRLHIISEMEKTISEARPDYKPNAPRIVEFTID
                     TDQIGAVIGPGGKIIQEIQRESGATITIEEKNNKGYVQIAAVGKESMDKALERVKAIV
                     AVPEVGSVYKGKVKNITTFGAFVEIMPGKDGLLHISEITHERLATMDGVFKEGDLVEV
                     KLIGVEEKSGKLKLSRKVLLPKPERQQGDAGKPNDGKPRERRMPKENQNS"
     gene            complement(5963..6232)
                     /gene="rpsO"
                     /locus_tag="PKY09_04810"
     CDS             complement(5963..6232)
                     /gene="rpsO"
                     /locus_tag="PKY09_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009414989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNB12210.1"
                     /translation="MIVTSERKKELFKQFGGNDKNTGNSEAQIAMFSERINHLTDHLK
                     IQPKDYATQQSLIKLVGKRRSLLNYLHKTDITRYRKIVADLGLRK"
     gene            complement(6379..7287)
                     /locus_tag="PKY09_04815"
     CDS             complement(6379..7287)
                     /locus_tag="PKY09_04815"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNB12211.1"
                     /translation="MKKIITTVITVAFAGSTMAQTSTPVDTSWKKGGMGSINFNQVAL
                     SNWAAGGENSISGAAFLTLFANYAKDRLAWDNQLDLAYGLLKSGDAKVRKNEDKIDLN
                     SKLGYKMSHDSKFYYTFLFNFKSQFANGYDYKNDTLLKYPISKFLAPGYLLYSIGIDY
                     KPNDAFSLYLSPLTGRTIIVNDDMLSQAGAFGVDPGKKSLTQLGAYLRAMYKKDVFTN
                     VNFQTKLELFSNYLDNPQNIAVNHEILIAMKVNKYITANIGTQMIYDDIIPVTVIKEK
                     SGVKEAKTGPRLQFKEVFGIGLSYKF"
     gene            complement(7376..7804)
                     /gene="mscL"
                     /locus_tag="PKY09_04820"
     CDS             complement(7376..7804)
                     /gene="mscL"
                     /locus_tag="PKY09_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNB12212.1"
                     /translation="MGFIKEFKDFAMRGNLVDFAIGVVVGGAFGKVTTSFVDGIVMPV
                     VGKLVGGADFSELKYKIQDGSKEVLDAAGNVTTKAVPEVYIKYGEFITVCLNFLIIAF
                     VMFLVIKVMNNLKKEEPAPAPAGPTKDQELLGEIRDLLKK"
     gene            complement(7925..8302)
                     /locus_tag="PKY09_04825"
     CDS             complement(7925..8302)
                     /locus_tag="PKY09_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB12213.1"
                     /translation="MKIINTTMAPAPIGPYSQAVQCGNILFVSGQIALDPANGQLITT
                     SISAQTTRIMQNIEAILHAAGLNFSNIVKTTIFLTSMSDFDEVNKVYGSYFKDNFPAR
                     ETVAVAGLPKGVNVEISVTAEVS"
     gene            8410..10179
                     /locus_tag="PKY09_04830"
     CDS             8410..10179
                     /locus_tag="PKY09_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB12214.1"
                     /translation="MHSFLKSLTTILLFYFPVLTFASDCKPPVNITITDVTSSSAQVS
                     WNIVKPGSSYQLQIITTSGKSNTLLASNNSLQLTALYPMTTYMVSIRSICGDDTSSES
                     DVISFTTKHVSVKDSIVRGAYLQMLTENSVVIRWRTFVSENSSVRFGKTMQYGKIKED
                     TVNVNEHTVLLTGLQPGTKYFYTVGSTSKDKQGDEENYFITAPKTGTIQPVRIWSIGD
                     FGTGKPPQLQVRDSYLRYTKNHPADIWIWLGDDAYSYGYDAQFTYKVFNPYKTILKHL
                     PVFPSIGNHDYAKKGYLDSASLGTDFPYFKILDCPTQGECGGVPSKTEKYYSYNYANI
                     HFIVLDSYGARSDKESEMYKWLNADLKANNARWTICYFHHPPYSRGTHNSDKPGESTD
                     IRQNIIPLLEKYKVDLVMSGHAHDYERTFLLHGHYGKQATLTQDMIVDKSDGNSPYYL
                     KSAPAYKGTVYVVNGVGGQGGNVKTQGDWPHKAMCSYSKQHYGSMIIDVNADTLSAVF
                     LTSTDTIFDRFKIVKSDNQKFKKNQKTGYLNLQNQNINKFNEAKRGKTGKQFSDAGRI
                     SEVEQKSFTTTYRPSVNYKMQQY"
     gene            complement(10130..>10390)
                     /locus_tag="PKY09_04835"
     CDS             complement(10130..>10390)
                     /locus_tag="PKY09_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12215.1"
                     /translation="PFKNLSSNFIVMQDYVNDSFSEYLKTIYGRQIRFIDLQMNQKED
                     RVSLSWHLTQKEKLYVVQEGNNELNKFNIAAFCNLLTDDKSW"
     assembly_gap    10391..10490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10491..11913)
                     /locus_tag="PKY09_04840"
     CDS             complement(<10491..11913)
                     /locus_tag="PKY09_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12216.1"
                     /translation="MNIKDRLKIFWLSFPVQLVIMHVKKSQLLLVYWLILFACVTQNF
                     GNRFGIPYLFLDPEYMGKVSWLAFFIIGICLGVFIMAYNISSYMLNSFRFPFLACLYK
                     TFEKYCYNNAVLPVMFILTYIGGIYYFQLKNQLLPISIVTIQVLALLMGVGFVIFSTL
                     KYFQHTNKDIYKLFGVATHDGDHDNVKFVSPIRDNKLKRQRRRGWKVETYLTFPFKLR
                     LVRSTSHYKSFMLASVFRQNHINAAVLEMIIFLLFILLGLFRDYSVFRIPAGGSILLL
                     FTMIIMIGGVFRYWLRGWAYTALVGLFILINFLSGLEVFNFKNKAYGLDYDGAQPEYS
                     IPKMEEILSHYRLQKDYETGLVSLNNWKKKWNDAGVEKPKLVVLSVSGGGVRSALFTF
                     NAMLELDSALNGQLLRYTQFISGSSGGLIGASYYRELYLRNKGSQKILENKETYLKNI
                     SKDVLNATAFSFIISDLFLNFQQF"
     gene            complement(11959..13641)
                     /gene="ftcD"
                     /locus_tag="PKY09_04845"
     CDS             complement(11959..13641)
                     /gene="ftcD"
                     /locus_tag="PKY09_04845"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNB12217.1"
                     /translation="MKQLIECVPNFSEGNDLTIIKQITDSIESVEGIKLLNVDPGKAT
                     NRTVVTFVGEPAAVVEAAFKGIAMAATLIDMSKHKGEHPRMGATDVCPFIPVANISMQ
                     ETVEWSKKLAARVGNELHIPVYLYEEAQENKERSNLSIIRAGEYEGFFRKIKQKEWKP
                     DFGPAELPANSGATVIGARDFLIAYNVNLNTKSVKRANSVAFDIREAGRVKTDSKGKK
                     ILDDAGNEIRIPGKLKGVKAIGWYIEEYGIAQVSINITKFRETPLHIVFEESARSAYE
                     RGYRATGSELVGLVPLQAMLDAGKYFLNKQRLSSGVSDAELIHIAVKSMGLDELSPFT
                     PEKRIIEYVMESESKAVLANMRLNAFVDETASESPAPGGGSIAAYCGSLGVALATMVA
                     NLSASKKGWEERWNEFSDWADKGQKIKKQLLHLVDEDTVAFNKIMDAFKLPKTTDQEK
                     EDRKQAVAEATRYAIEVPLSVMKISLQSMELIEAMATNGNPNSVSDAGVGAMCARTAV
                     LGAGLNVRINAAAYSDKTFVETVLKQAAEMENKAIANEKNILDVVNRKISGL"
     gene            complement(13811..14884)
                     /locus_tag="PKY09_04850"
     CDS             complement(13811..14884)
                     /locus_tag="PKY09_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNB12218.1"
                     /translation="MSHLVVYSREDINQETRTRKNETKVGENVQVLNKSDATLIERLN
                     QCDAEYVLLGLPEDIGIRANGGRGGAYSAWKPALFSLLNVQSNCFGDGSNILVLGHIN
                     FDDLMLAADGINFKTEKGISKARELVGQIDERVFEVVKAIIQSGKEAIIIGGGHNNAY
                     PNIKAAAIGLQTSGVIKSESINCINCDAHSDFRIMEGRHSGNGFRYAYEHKFLNKYAI
                     VGLHESYNAGNVLQFMMDRPERFSLSFFEDIFIRENISFTEAVDKAIEHVKDNFCGIE
                     IDMDTIQNIPSSAKTSSGISTIQARQYINRAASKLNACYLHIAEAAPVLSHIKTDNKT
                     GKLIGYLIADYIKARSTFKNNKV"
     gene            complement(14887..16134)
                     /gene="hutI"
                     /locus_tag="PKY09_04855"
     CDS             complement(14887..16134)
                     /gene="hutI"
                     /locus_tag="PKY09_04855"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNB12219.1"
                     /translation="MIVILNIKGLCGILDKDILIKRGQDLRSYMFLPDAFLVIKDGLI
                     KSYGTMTEWESVKEEYSAYEPVQANGKYLMPGWCDSHTHLIYAASREEEFVDRINGLS
                     YEDIAQRGGGILNSSKKLAAASEEDLFLSAMQRIDSMIAKGTTAIEIKSGYGLSLESE
                     LKMLRIIKRIKESSSLIIKSTFLGAHAVPEKFKNNKAGYVRHIIDDMIPAVAAEKLAD
                     YCDVFCERNYFSQQETEDILNAGAKYGLIPKVHANQMSRSGGVQAGVNCNAISVDHLE
                     FVEDEEIAALKNSTTMPVVLPGAAFFLNLPLPPARKMIDAGLPLAVASDFNPGSSPSG
                     DMNFMISLLCVQYKLNPEEAFNAATINAAYAMNVSHLTGSISVGKRADFFITKAISSP
                     YIIPYSFNENVIESVYVKGKKVV"
     gene            complement(16180..18342)
                     /locus_tag="PKY09_04860"
     CDS             complement(16180..18342)
                     /locus_tag="PKY09_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB12220.1"
                     /translation="MEKKIFSLFFFALATLNLYAQKNFTYADIWGSSQFAARQVASLK
                     SMNSGDTYSNTDRAGNLIRYSFKTGNVIDTLIKIDELQASIKDFRYSDYSFSNDEKKV
                     LLTTASEAIYRHSTKANFYVFDFKSRKLTAVSEKGKQMYAQFNPTGSMVAFVRDNNLY
                     LKNLYDLSEKMVTNDGKKNFIINGALDWVYEEEFSFSQGYQWSNDGKYLAYYRFDESN
                     VKEFTLTYYDSLYPKEEKYKYPKAGEENSVVDIYVYDLSSGRSVRMQTGDEKDQYIPR
                     IKWTEKVGQLCVLRMNRHQNNLDYLLCNAVSGKTTLLMNENSNTFIEITDNLVFLNNG
                     TQFIYSSDKSGYNQIYLRSLSDGSEKMLTNGGDVITFYGYDEKTKNCFYQVADPTPME
                     RVVYSVDLNQKKKLLSPANGTASASFSSNFKYSVITHSTIDKPYICYVQDSRGKKIRV
                     LENNERVVKNLESYTISKPEFFKFTNSENISLNAWMITPPGFKANENKKYPVIVYLYG
                     GPGRQTVLNQWGGTNFLWHQMLAQNGFIVVSVDNRGTPGRGKEFANSIYKNMGHYETI
                     DQIEFAKYLRTLSYVDSTRIGVHGWSYGGYLTSLLMTKGADYFKTGVAVAPVINWRYY
                     DSIYTERYLSLPAENPKGYDDNSPVFFADKLKGKYLLIHGLTDDNVHFQNSAEMVIAL
                     VKNKKQFDTFYYPNQAHGISQSRGHLYEMMTEYFKKNL"
     gene            complement(18503..18709)
                     /locus_tag="PKY09_04865"
     CDS             complement(18503..18709)
                     /locus_tag="PKY09_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12221.1"
                     /translation="MAVIALSKAEMQSIQKIGSMLSGLSKIIGEDQLNEFKLYDEIEA
                     EKAAAQSASRKQAYSSKATIEKLI"
     gene            complement(18817..19167)
                     /locus_tag="PKY09_04870"
     CDS             complement(18817..19167)
                     /locus_tag="PKY09_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNB12222.1"
                     /translation="MHDSENNGNSREVIFSKAVKAGKRTYFFDVKSTRANDYFVTITE
                     SKKKFNMGDDKPFYEKHKIFLYKEDFEKFIEGLNEAVSHIKNGNAGKSDHDNNSSGGS
                     SNPYTDISFDEETK"
     gene            complement(19323..20216)
                     /gene="meaB"
                     /locus_tag="PKY09_04875"
     CDS             complement(19323..20216)
                     /gene="meaB"
                     /locus_tag="PKY09_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNB12223.1"
                     /translation="MSFNLNQAEISQGNYRSLAKVISQVENAADGAMDLLRHLKINYD
                     IPVIGFTGPPGAGKSSLLNALLAKMADKWRIAILAVDPTSPFTSGSILGDRYRMNDYF
                     LHQNVFIRSLATRGNLGGLSARTIEITDVLRSCAFDFIFIETVGVGQSEVEIAALADT
                     TVLVLNPGAGDDIQALKAGIMEIADVFVINKADKEQADVLVKHIQNMLRLRNQTRYSP
                     IFKTVATEGTGIDLLTECLVDKTRKHSDKLNLFYEKSIRLIISNKMRDFDYQSLKIKL
                     QTATDSKDFNLYNFLHENYFK"
     gene            20300..21067
                     /locus_tag="PKY09_04880"
     CDS             20300..21067
                     /locus_tag="PKY09_04880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014243099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB12224.1"
                     /translation="MVKISGVIITFNEEKKIEQCLKSLLPVCDEIVVLDSLSTDKTEE
                     ICSRYEVRFIKQKFLGYIEQKNHALTFASNNLVLSLDADECLSESLQKSIEQIKNNQT
                     TTAYSMNRLTRYCDKWIYHSGWYPDVKVRLFDKTKAQWGGTNPHDKIILSERTSVQHL
                     KGDLLHYSIDSIEQHIQQATKFGIIAAKAKFAKGQSSNGFNTLLHTAGRFLKTYILKA
                     GFLDGYYGWLIAKYSAKSVYLRYSLLEPRNHEKLNSL"
     gene            21067..22170
                     /locus_tag="PKY09_04885"
     CDS             21067..22170
                     /locus_tag="PKY09_04885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB12225.1"
                     /translation="MKVLHISTATGWRGGEQQAAYLINQLGNLQTACFLLCPKDAAIM
                     QRLNNSNCKIEVFQKGKALNLSLARKIKTLCKENQIDLIHTHDSHAHTAAFLSSWLFG
                     NKCPVVVSRRVDFAVSSSLLSHLKYNHSSIKKIICVSVAIRQITAPAIKRKKILEVVH
                     SGVDTDKFIFGKTKLHQEYRLPKETFLVGNVSALADHKDLFTFIDTAEIILQQAQHIH
                     FFLIGDGSEMNALKTRVTEKKLTANIHFTGFRNDVNELLPELNLFLMTSKTEGLGTTI
                     LDAFAAKVPVVATNAGGIPEIVRHEQTGLIAPIGNAQQLAKLVLEVMNDSNLANTLAT
                     NAQQLVADFTFRRTAEKTKAVYTQVLQEENQKV"
     gene            22170..23999
                     /gene="uvrC"
                     /locus_tag="PKY09_04890"
     CDS             22170..23999
                     /gene="uvrC"
                     /locus_tag="PKY09_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNB12226.1"
                     /translation="MAVKNEHIKAILEGLPNEPGVYQFFDKNEKIIYVGKAKNLKKRV
                     YSYFSKDIFESGKTAIMVRQISDLRFIITETELDALLLENNLIKKHQPRYNILLKDDK
                     TYPWICIKNEPFPRIFYTRRKIRDGSQYFGPYASVKMINILLDMIHRLYKLRNCNLNL
                     TPENIQNKKFRVCLEFHVGNCLGPCEGRQSAEDYQQGIDNIRHILKGNINQVIRILQD
                     EMQTASQNLEFEKAQQIKSKLDTLEQFRSKSVIVSPVINNVDVVTIVSDENYGFANYM
                     KVINGAILQSQTIELKKKLSETDTDLITFALTELRQRFESDATEILVNVMPDTYPADV
                     EVSVPKIGEKKHLLSLSEKNARYYQLEKLAQYDKLNPEHKTDRLMEKMKADLRMMEQP
                     RRIECFDNSNFQGTNAVAAMSVFINGKAAKKEYRHFNIKTVEGPDDFASMQEVIYRRY
                     KRVLEEKQPLPQLIIIDGGKGQLNAALTGLEKLGLNKKIQTIGIAKKLEEIYYPGDSV
                     PMYLDKKSETLKIIQQLRDEVHRFGITHHRNKRSRNFLQSEILQIPGIGNATFEKLII
                     QFKSVKKIKEATAAQIADVIGKQKAAIVVNYFNSEKQQADEVN"
     gene            23986..24954
                     /locus_tag="PKY09_04895"
     CDS             23986..24954
                     /locus_tag="PKY09_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB12227.1"
                     /translation="MKSTKIFYLLVVYVLLQLGWWSYLLVKLNSELGNEKLRYLETLP
                     QTEATILQENAIRKKLKEKYLMVAGEGLVFLSLLGIGIFYARRSFKKEFQMALQQKNF
                     MMSITHEFKSPIAGIRLSLETLLRRDVEKQQRDKILGRALLETDRINFLVENILTATR
                     IEAANMDFQFADINLSETLSDLMTILQQSANSHNFKLKIENDIHVTADKNAIHSLILN
                     LIENAQKYTPAGSDIEVKLFQIDKDVFMQICDNGPGIADIEKRKIFEKFYRIGNEETR
                     TTKGTGLGLYICRFIVLKHKGRIAVQDNYPQGCIFEVVIPSLNNKI"
     gene            24957..26480
                     /gene="gpmI"
                     /locus_tag="PKY09_04900"
     CDS             24957..26480
                     /gene="gpmI"
                     /locus_tag="PKY09_04900"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNB12228.1"
                     /translation="MNKKKVVLIIMDGWGEGRKDKSNAVYEANTPFIDSCYDKYPHSE
                     LLTSGLNVGLPDGQMGNSEVGHLNIGAGRIVYQELALINKAVEEKTLHSNTVILTCLD
                     YVKRTGKKLHLIGLVSNGGVHSHINHLKALCDIAKDNDVKDVFIHAFTDGRDCDPKSG
                     LGFIKELENHLKISSGKIASVVGRYYAMDRDKRWERIKEAYDLMVNGIGVHFGNAVSG
                     IEASYKNNVTDEFIKPICITENNKPVATIQDGDAVICFNFRTDRCREITTALTQQDFH
                     EQNMHKLNLEYVTMTVYDDKFTGVKAIFSKDNLENTLGEVLQNANKSQIRTAETEKYP
                     HVTFFFSGGRENEFKGEKRILIPSPKVATYDLQPEMSALLLKDALVNELKKGETDFVC
                     INFANADMVGHTGVFTAAVKAVETVDACVNEIVQCGLEQGYSFLITADHGNADYMIND
                     DGSPNTAHTTNPVPLFLIQPEHEFNLKNGILADLAPTILQLMQVQQPAEMTAKSLLS"
     gene            26587..27492
                     /locus_tag="PKY09_04905"
     CDS             26587..27492
                     /locus_tag="PKY09_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12229.1"
                     /translation="MKKITLLLLMLSVNQLYGQSRTFFSLSGGILYYNGDLNDKSPVP
                     STKIFRNYGNAAIGIHLTRHVNLQLNYYHGSIEGADSLTKERDNKQRNLSFETRIDEI
                     SFQLSASLFSNRTKKLAIPYVFAGGGLFFFNPRARYYGSNPEYYGKLVSLQPLGTEGQ
                     YITEGDYPKPYKLYQAVFPIGIGTYIRINPKWRLKVEFSEHFTLTDYLDDASGKYPNM
                     EALSLTPKGELASYFADRRLNRTQDPTGRNRSNATKNDNYIHFGIGLVYNPQAQHPTR
                     YRGPGFWKKLFSGRKGWWGNAGSGL"
     gene            27568..28389
                     /locus_tag="PKY09_04910"
     CDS             27568..28389
                     /locus_tag="PKY09_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB12230.1"
                     /translation="MKILVPVDFTDITEKGFKYALSLPFANEIILFHAVDDIKKSKEA
                     QQQLSNITNKYSAPAHLTVQSKVKQGAIFEIIGNVSKEVNADLVVMATHGIKGLQHFL
                     GSHAMKLITHSKTPFIVVQQKDFSPLKKMLVPVDFTKEVKQELPAVIQYAQLFNASVL
                     LLKQNNHDPYIQKRSDLNTGYFEDELDNAKIKYELIEQTFSLDDKYEVLTKQAAAHNA
                     DIIVTTIEPDLDVADYVIGVEEQKIVANDLQLPVLCVNIRHFTVAGDQFSVSVQQ"
     gene            28532..28807
                     /locus_tag="PKY09_04915"
     CDS             28532..28807
                     /locus_tag="PKY09_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12231.1"
                     /translation="MSLVTCKEFNKLSHDEQTWYIWNGAIHLHIREVYGYRVNLFYFN
                     EFYIELWYSINEKSIKKIKSFDNSALLAPYLETIKIDSLLNIDSGFE"
     gene            complement(28810..29487)
                     /locus_tag="PKY09_04920"
     CDS             complement(28810..29487)
                     /locus_tag="PKY09_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNB12232.1"
                     /translation="MKHFRKLCGLKIMAVMVLLLSSFKAPDFTLKLAKLKYAGGGNWY
                     VSPTALHNLAVFCNQNINTNISPEEAVVEPGSPDLFNYPFVHMTGNGRFVFSNQEAEN
                     LRKYLIAGGFLNINDSYDMDKFVRPEMKKVFPELDFVELPFTHPIYHQRFNFPNGLPK
                     IHEHNGKAPQGFGLIYQGRLVCFYEYESDIGDGWEDPEVHNDPPEMRQKALQMGANIL
                     QYVFSGQ"
     gene            29587..32505
                     /locus_tag="PKY09_04925"
     CDS             29587..32505
                     /locus_tag="PKY09_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12233.1"
                     /translation="MTLRILLVAIFAPLMLNAAHDHNGFDRNEDPNLKVFVKNRKRLP
                     DVNRQESLAQQKSWQQFSEQNKGWSVVFSEETGLPHNAFGKPISVGLPLDAQSTAWSF
                     ISTKLNAFGLPLNDLYFRNTAQNKKYFYVNYIQRYNGLEVLWSKVQIKITKDGKVNQF
                     ICDAYKDIAVNIAPALSSQTATAAATNGLNLTINGTSVNNDLKILPVPDEGKNHYHLV
                     YEVTVKATDAESFPREYYTLVDAHNGEVLYRHNKINYFAANTDVNVTATVYPTQPYLP
                     TAVEPLANMNVTISGVPFLTDANGYLGLPNTSPVTATFRLEGSWANVKTGATTPQFTA
                     TLNPGANNITFSAANIKELSAYNSVNEIHDYYVTKLLGSGAEAIMDFQMQTIVDVAGN
                     CNAFYDGNLNFYAAGGQCNATSIINDVVYHEYGHGINYDLYSYYGGIFSNGALGEGYA
                     DTWANGLTEDPILGIGFYSNSTTNFVRRYDIGRKVYPDDIMGEVHADGEIIAGAWWDV
                     GVNFNNVQQRQDLYVQTFAATIDYPDGQEGQLYTDILVEALTIDDNDGDLTNGTPNYC
                     AITSAFGLHGINMFVVGKLITHNSVLSAAGQQSINLNVSGSSTFANTTGTSINGYYSL
                     NHSATWNPISFTYNGSIFQGNIPAQPNGTLISYYLDATDQCGTHFSTTPSNVTDTNPN
                     VPYYILVGYNLVDNDYFDAGNSQGWTLGMPGDNATTGQWIIASPVGSLLNPGGSPPVY
                     VQPPVDHTSTANNLCAVTGNASPNDGAGTNDIDDGKTTLLSPNFDLSAYSNPAISYWR
                     WYTNDQGADPGKDYWQVSVSNDGGTTFVPLENCIVADHSWRRFAFRVADYLTPTANMR
                     LKFIAEDANDGSLVEALLDDIEIWSAEPVGIAETPILSWYAYPNPANEQLNFGWKGNG
                     ATVDVKLFNTVGQIVYQHQFSGNTVSETVSLKGMAEGIYTLQMSGENIFKSQKISVIH
                     "
     gene            complement(32573..33025)
                     /locus_tag="PKY09_04930"
     CDS             complement(32573..33025)
                     /locus_tag="PKY09_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12234.1"
                     /translation="MNYMLNRMNCCALVLVVAMAGSCKTNKNTTAETPKQEPAATNPR
                     VDLPDSMYRVIVSFISIGAGTDASAKEKLDQVVQQYQTDAGMPIAREEIAWGREGEVD
                     FCFKLLGQDKKSKDSFVQQLRDAFKGNNLVQITENTPALHQARPSFNK"
     gene            complement(33009..34502)
                     /locus_tag="PKY09_04935"
     CDS             complement(33009..34502)
                     /locus_tag="PKY09_04935"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNB12235.1"
                     /translation="MSELYQQYVTHLQKIADINYSSAVLQWDQEVYMPPKGAHYRAQQ
                     LSTLAGYAHELITDKRYEEIIEKLHDDTTKLSPVQCANVLESYRVFRQQKKYTTEFVV
                     ALNQCISKSFNAWQQARQENNFKLFEPHLQELIKFKRQESELLGYEQHPYDAHLNLYE
                     TGLKTSFLETIFNQVRQKLVPYIQQLVQKPQPDDSFLFKQYNKDAQWHFGLDLLKQMH
                     YDFDSGRQDISTHPFTTSFSANDVRVTTRISENDLFEMIGSCIHEGGHALYEQGLPAD
                     DYGLPSGEYLSLSIHESQSRLWENHVGKSQYFWNYNFKTLKNIFPQQLHDVSERSFYK
                     AINRIKPSLIRTNADELTYHLHVLIRFEIEKDILSGTLQAAQLPEVWNAKYKSYLNID
                     VPSDSKGVLQDVHWSHGSFGYFPTYSLGSFYASQFFHQANKEIPALMHHILYGDTMPL
                     LMWLRSNIHRHGRTILADELCKNVTGEVLNFNYFFNYAKTKYDELYA"
     gene            complement(34505..34882)
                     /locus_tag="PKY09_04940"
     CDS             complement(34505..34882)
                     /locus_tag="PKY09_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNB12236.1"
                     /translation="MNRKILIFATISGLCAVALGAFGAHKLKELLSPQMLSAFETGVR
                     YQFYHTLALLFVALAANDNKMLNRAATFFMTGIILFSGSIYGMALSSIGGNTWAWLGP
                     VTPVGGLCFMTGWLFLIIHAFKK"
     gene            complement(34882..35418)
                     /locus_tag="PKY09_04945"
     CDS             complement(34882..35418)
                     /locus_tag="PKY09_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12237.1"
                     /translation="MEELGIGSRIRHPEFGVGVVINVKPRTYITVFTERGKIEVAKTY
                     QNLEVIDAQPPAGDPVSFADMERVFTNIIKKYSDVQETVPLHGRWIGGKVVLHPNNPS
                     QKPKEIPMDVFFNKIIMIRDRLRVMEQRINAHDKLTEEDKINLQQYLTRIYGSLTTFN
                     VLFKDEEDVFVGEKGNKE"
     gene            complement(35437..>35652)
                     /locus_tag="PKY09_04950"
     CDS             complement(35437..>35652)
                     /locus_tag="PKY09_04950"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HNB12238.1"
                     /translation="NDSTLGGFGYNILVDGKIMVHQPNIPAVMGNRGFDTDEDASKTA
                     ALVSFKLKNNIMPPSVTIQELDSIGIK"
BASE COUNT        11176 a         7081 c         6271 g        11024 t
ORIGIN      
        1 gaatcttact tttttaaaaa accttgtgtc attttacgta acgaaacaga atgggtagag
       61 ttggttgatg caggcacagc tattattgca ggtgccgatt ccgaaaaaat tattgctgcc
      121 tatcaatcac taaaaactgc atccttaaat tttccaccca ttttcggtga tggtaaagct
      181 gctgaattta tttgtaatga aattttagct gcgtttaatt agttattttt acagaaatat
      241 attttgcagt gaaacacata ttgtccaagt cgagttatat tcgcagcctg caatgtctaa
      301 agtctttata tttctataag tttcactaca aagacaggga tccaattagt gcagagcagc
      361 aagctaagtt tgatcgcgga catcaatacg gaaaaattac acatcagcta tttccgggag
      421 gcattgatgt ttcaccgcct actccatttg agtatgcaac atctgcaaaa caaacacaag
      481 ctttactgct caaacagcaa cctgtaattt atgaagctgc ctttattgca aatgaagtgg
      541 ttgtggcatt ggatattctg gtgcgcaaag gtgggttact ccatgcgttt gaagtaaaaa
      601 gtactactgc catccatcct gccatactca atgatgcagc actgcaatac tttgtcataa
      661 ccaatgcagg ctatggattg gaagatttca gcatcataca ccttaggcct gattataaag
      721 atttgcctgc tgatgatata ggtgctttgt ttgtgaccga aagtgtaaaa gagtattgtg
      781 tgtcacaaca acaatttgtt gaagacaaca ttttatctgc taaggaaatc attcgtaaca
      841 aaaaaatgcc tgtaaaagaa cagggcgaac attgtaattc gccctacccg tgtgatttta
      901 agaatatttg taataaaagt aactcctacg cttcaggaag tttgtttgga taaattaatg
      961 catcaccgta aaccgtgcag tgcttgaatt ctttttatct gacatcttta atatgtaaac
     1021 gccattatcc aaatctctga caacaatttg aaccttatta ctattagcct gaatctcttt
     1081 cactactctt tcatttaaat caaacacagc cagtgttgtc atttccaacg gtgacggaat
     1141 ttgcactgtt aatatatcag aaaccggatt tggcgaaaca gcaactacca actgactact
     1201 caattctttc actcctacaa aaggtgaagc tcccagatta agccattcat ttgctcctgt
     1261 tgttccaact ccattgtagc caccaacaac aaccatatat aaactgtcat tgctgactac
     1321 acctgtaaaa ttacttacgt tactacatgc agttggtttt ggcggaccca caagccattc
     1381 attgttattc aaatcataag cccacacatc atcttttact tctgtgccga caccggtagg
     1441 atcacctgca gcaaatacta catagggcaa gctgttttta aatgttccac cacctgcaaa
     1501 acgtgtcatt gtgccaccgg gatatggtgc tgctgcctgc catacaatac ttgttggatt
     1561 tgcaggatct atctgcccta tccatgtttc atcaattgca tcttgtaaag tctgactgta
     1621 accgcaagtt actacaataa aattattata aattcctcct ctgaagcctg acacagccgt
     1681 tccgggctta ggtgtaccca tactccaact gtctgttgcc gggttgtata tctgaaccgt
     1741 attcaaatca ttagttccat cataaccgcc aataaaataa attaatgaat cgttatatat
     1801 gccacttgca taatcaccca ccggctgtgg cattaccgca cctgatgtcc aactgttggt
     1861 ctgtggatta taaatatagt tggtaggaga aggaacaaag ccgctactat aaccaccaat
     1921 tgcatacatc atattttgta tgccatgcat tgaaaactga taaactccaa taggcatatc
     1981 cgtggcagaa gtccaattgt ttgtcatctc atcaaatcgt tcattcttag gttgaataat
     2041 acttagatca ctaccgccat cagaaaataa atatcctgaa tctaaaggtg catttacaga
     2101 aggaataaat gccgatgctc ctccgaaagt tgctattgca gctgatgttt tacttgtcca
     2161 tccatagttt ggaagttcat taaaagcagt tgtagattta ttcaatgtat catttgataa
     2221 gtcgctgtcg gttgctaatt gagaataaac agtaatgtta taacttcctg ttgctccggg
     2281 tgtccatgga tcgaaactaa tttgttgtgt ttgattgaaa gccaaattac ttactgtttt
     2341 agtagatgta tatccgccac ttatatccat tgtgacatta aatgtctgtg tagtaagtcc
     2401 attattcaaa accgttgcca taggagtttg agtgcctgta ccaacagcac tgcggacatc
     2461 aatactctta acggttgcat cattggcagc aatagtcatg ggacctgtcc atttgtaaat
     2521 aggcgaattc agttcaccac ctccactcca accggtagtg gcatttacga agtcgcaatc
     2581 agaatgtatg aggttatcca tgagacgcca attactacca ttatcaacac tgaatgctga
     2641 gccgaagatt gtataaggtg aagtaatcac aaaggtggaa tcggtaccgg ggacaaaacg
     2701 aatggattgt ttcattgtaa atccggcaga tgaggtaggt aggagattcc atgttgcacc
     2761 accatcatta gtgataatta aatctgttga tgtggttgca ttatcagcct cacaggcaat
     2821 accaaagttt gcatctctga aggttattgc accaatatat ccggtataag gtgtactgct
     2881 tactgtccaa ttagcgcctc tgtcagcaga cttataaatg cgccctttat tggttccgaa
     2941 ataaacggta ctgtcgccaa aattggtata aacatccgta ataccaaatt caccggtcag
     3001 gtttgctgca atgtttgcct gcggcacacg aatccagttg ttgccaccat ctgctgttgt
     3061 gtaaatttca aaatagccac cattaggatc acccatgcaa aagccttcgt tcgcattaaa
     3121 gaaatgtacg acattgggaa agccattagg tgctgtaaaa gttgctgttg tttgttttgt
     3181 ccaggaaaca ccaccatcat tggttcgtaa aatacttcct ccaccgctga cattaaacat
     3241 agctacccat gcggtatcgg cattgagtgc cataatacat gaaggaccat gattggctgc
     3301 attggtaatg gtacctgctg tccatgtttg accgccattg gttgtccgtg taaattcctg
     3361 aacgggattt tgctgtgaag tgcgatcaga agctaaaccc cataccacat tggtatcaac
     3421 tgcatcaata aactgtacgt agttgtttgg gttgatgttt gagggttgca ctatccattg
     3481 ctgtgcatct aactgattac ttaaaagaca aagtgaaacc catgcaaaaa agagtaaaga
     3541 ctttttcata attttttgat tttagtggtt caaactgttt tgatttatca aacaaatata
     3601 aaaaaatcat tcaacggaca ataaaaagtt tttacatcct caaaaacaag tcaaaaaaaa
     3661 accggcctta cgaccggttt agatattatg agttttgatt ctctttgggc attcttcgtt
     3721 cacgaggttt gccgtcatta ggcttgccgg catcaccttg ttgacgttca ggttttggca
     3781 acaacacttt gcgggataat ttcaacttac cacttttctc ttctacacca atcaatttca
     3841 cttctactaa atcaccttct ttaaaaacac catccatagt tgctaaacgc tcatgtgtaa
     3901 tttcggatat atgtaacagg ccatctttac cgggcataat ttcgacaaat gcaccgaagg
     3961 ttgtaatgtt ctttactttt cctttatata ctgagcccac ttcgggtaca gcaacaatgg
     4021 cttttacacg ctctaatgct ttatccatac tctctttgcc tacagcagca atctgcacat
     4081 agcctttgtt gtttttttct tcaattgtga tggtagcacc actttcacgt tgaatctcct
     4141 gaataatttt tcctccgggt cctatcactg cacctatctg atctgtatca attgtaaact
     4201 caacaatacg tggtgcattc ggtttatagt caggacgcgc ctctgaaata gttttttcca
     4261 tttcgctgat aatatgtagt cgtccctgac gtgcctgctc cagtgcctgc gccattactt
     4321 catacggcaa ccctgttact ttcaaatcca tttgtgttgc agtgatacca ttacgtgttc
     4381 ctgcaacttt aaaatccatg tcgcctaagt gatcttcatc accaagtata tcgcttaata
     4441 tagcataacg tccttttcca tcgctaatca atcccatagc aatacctgcc acagttgatt
     4501 tcattttaat acctgcatcc atcaatgcca tacttcctgc acatacagtt gccatagaac
     4561 ttgatccgtt cgattcaaga atatctgata ctactctgat ggtatatgga ttttctgatt
     4621 ccggtggaag tgcttttgtt aatgctctta atgcaaggtt accatgtcca acttctctgc
     4681 gacctgttcc acgcattggc tttgcctcgc ctgttgagaa tggaggaaag ttataatgca
     4741 acaaaaactt tttcgttcct tcaatcatgg caccatcaat aatctgctct tccattttac
     4801 caccaagagt aactgtagtg agcgactgcg tttcaccacg tgtaaataca gctgaaccat
     4861 gtgccgttgg caagtagttt gtttcactcc aaattggacg aacatcagtt aatccacggc
     4921 catcaagacg ctttttctct gccaataagg cttcacgaac agcatcatac tccacctcat
     4981 gatagtagtg gtttatgaaa gctgattttt cttccagttc ttcctctgta aaacgggttt
     5041 taaacgcttc tacaattgct tcaaatgatt tttttctttc gtttttattt ggattacctt
     5101 gtaaggccac ctgataaact tgctgataag tttcatcacg aaccaatttt cttaaatctt
     5161 cgtcatgctc ttcgtggttg tattcacgct tggttttacc accaattttt tcgagtaaat
     5221 ccaactgtgc tttgcactga agtttaattg cttcatgcgc ttcttttatt gcttcgagta
     5281 tggtggcttc gcttacttca ctcatctcac cttctaccat cacaatattt tccattgagc
     5341 cggctacaat taaatcaagt gtagcacgcg caatgtctga aatatacgga ttgataacca
     5401 ttgcaccatc aatttgtgcg acacgaactt cagaaatagg tccgttaaat ggaatatcac
     5461 ttatggttaa tgctgcagaa gctgcaagtg ctgcaagcgc atcaggataa atattgctat
     5521 ctccggaatg taatgtaacc aatacctgtg tatcggcatg aaaattttca ggaaacattg
     5581 gacgcagtgc gcggtcaata agtctgctga taagaatttc atattctgat ggtcgtgcct
     5641 cacgcttaaa aaatcctccc ggaaagcggc caactccggc atacttttcc tgatagtcaa
     5701 ctgtcagggg cataaagtcc actccgggca tagcatcttg cttggcacat gctgttgcta
     5761 aaagcataca atcgcccatt cttacaacaa ctgaaccgtc agcttgcttg gctaatttgc
     5821 ctgtttcaat tgaaatctct ctaccatcag gtaaagtaaa tgtgtgttta actcctaaat
     5881 tcattttatc gtattattta aaaacaaaaa ggcaattgtt taccattgcc tttttgaaat
     5941 attattgtat tcaacttaat ttttacttcc ttaaaccgag gtcagctaca attttacggt
     6001 aacgggtaat atcggtttta tgaagatagt tcagcaaact tcttcttttt ccaacaagct
     6061 taatcagcga ctgttgtgta gcataatctt ttggctgaat tttaagatga tcagtaaggt
     6121 gattgatgcg ctcagaaaac atggcaattt gtgcttcaga attaccggta tttttatcgt
     6181 ttccgccaaa ctgtttaaac agttcctttt ttctttcaga tgtaactatc attggtatag
     6241 actatgattt tgtaaaataa gtgcgcaaag ataggttttt atttgattat aatatttaat
     6301 ttcctaaaaa aaataaagcc ccatactttg acagggcatt attttagtaa ttatattgtg
     6361 attcaattag ttatgtaatt aaaatttata ggaaagacca ataccgaaaa cttctttgaa
     6421 ctgcagacgt ggacctgttt tagcttcctt tactccgctt ttttctttga ttacagtcac
     6481 aggaatgata tcatcataaa tcatttgcgt tcctatgttg gcagtgatgt atttattgac
     6541 cttcatggca atcagaattt catgatttac agcaatattt tgtggattat caagatagtt
     6601 actgaacaac tctagcttgg tttggaagtt cacatttgta aatacatctt tcttatacat
     6661 agcacgtaag taagctccta attgcgtcaa tgatttttta cccgggtcaa caccaaaagc
     6721 acctgcttga gaaagcatat catcatttac aatgattgtg cgacctgtta atggcgacag
     6781 atacaaacta aaagcatcat tgggtttgta atcaataccg attgaataaa gcaaataacc
     6841 gggagctaaa aatttagaga ttggatactt tagcaaagta tcgtttttat aatcatatcc
     6901 atttgcaaac tgacttttga agttgaaaag aaaagtgtaa tagaacttac tgtcatgcga
     6961 catcttatat cccagctttg agttaaggtc aattttatct tcattttttc tgactttagc
     7021 atcacccgat tttagcaatc cataagccaa atcaagttga ttatcccaag ccagacggtc
     7081 ttttgcatag ttggcaaaca gtgttaaaaa tgcagctccg gatattgagt tttcgccacc
     7141 tgctgcccag ttacttaatg ctacctgatt gaagttaatt gaccccatac cacctttttt
     7201 ccatgaagta tcaaccggtg tagaagtttg cgccatggtg ctgcctgcaa atgcaacagt
     7261 aataacagtt gtaataattt ttttcattgt aatgatttta atttttaatt ttttaaagct
     7321 attatataaa gcaaaagagg gattgttgtc aacccctccc ccctattcat aaattttatt
     7381 tttttagtaa atcgcgaatc tcgccaagca actcctgatc ttttgttggt ccggcaggtg
     7441 ctggtgcagg ctcttctttt ttcaaattgt tcattacttt aataactaaa aacattacaa
     7501 aagcaatgat taaaaagttt aaacagacag taataaactc accgtattta atatacactt
     7561 cgggcacagc cttagtagtt acattacctg cagcatctaa aacttccttg ctgccatcct
     7621 gaattttgta tttcagctcg ctgaagtctg caccacctac taatttaccc acaacaggca
     7681 tcacaatgcc atcaacaaac gatgtagtaa ctttgccgaa agcaccgcct actacaacac
     7741 caatagcaaa atcaacaaga ttgccgcgca tggcaaaatc tttaaactct tttataaatc
     7801 ccataatatt tagtttttaa atgttagtca gaaaaatttc actgcaaatg tatcattcca
     7861 atcattgaac atgacacatt gcactacaaa aattcaaaac ccttcacaaa atttttagat
     7921 tctattatga aacttctgct gtaacagata tttccacatt tactcctttg ggcaggccgg
     7981 caacagcaac tgtttcacga gccggaaaat tgtctttaaa ataactgcca taaaccttat
     8041 taacttcatc aaaatctgac attgaagtaa ggaagatggt tgtttttact atgttggaaa
     8101 aatttaatcc tgcagcatga agtattgctt caatattttg catgatgcgt gtggtctgag
     8161 cagaaataga ggtagttatc agttgtccgt ttgctggatc aagagcaatt tgccctgaaa
     8221 caaagagtat attaccgcat tgcactgcct gactgtatgg cccaattggt gcgggcgcca
     8281 ttgttgtatt tataattttc atgttgcgaa agtatgaaac aatgataatt taattaagcc
     8341 aactgataaa agtcagattc ataataatat tcttatctaa ctttatagcc tgttttatca
     8401 aatcaagtta tgcatagttt tctgaaatca ttgaccacaa ttcttctttt ttattttcct
     8461 gttctaactt ttgcatcaga ctgtaagcct ccggtaaata ttacaataac cgatgttacc
     8521 tcttcatcag cacaagtttc atggaatata gttaagccag gttcatcata tcaactacaa
     8581 attattacta catcaggaaa gtcaaacact ttacttgctt caaacaacag cttacagcta
     8641 actgcacttt acccaatgac aacctacatg gtttcaatca ggtcaatttg tggagatgac
     8701 acaagtagtg aaagtgatgt aatttcattt acaaccaaac acgtcagcgt taaagactca
     8761 atcgtgagag gtgcctatct gcaaatgtta acagaaaaca gtgttgtcat aagatggaga
     8821 acttttgttt ctgaaaacag cagcgtacgt tttggcaaga caatgcaata tggaaaaatt
     8881 aaagaggata ctgtaaatgt taatgagcac actgttttgt taacagggct tcaaccggga
     8941 acaaaatatt tctacactgt tggctcaacg agtaaagata aacaaggcga tgaggagaat
     9001 tattttatta cagcaccaaa aaccggaacc attcaaccgg taagaatatg gtctattgga
     9061 gactttggta ccggtaaacc accacagctg caggtacgcg attcatattt acgttacact
     9121 aaaaaccatc ctgcagatat ttggatctgg cttggtgatg atgcctattc ttatggctat
     9181 gatgcacaat tcacctacaa agtttttaat ccttacaaaa ccatactcaa gcatctgcct
     9241 gtctttccat ctattggcaa tcacgactat gccaagaaag gttaccttga cagtgcttcg
     9301 ttaggaaccg actttcctta ctttaagatt cttgactgcc ctactcaagg cgaatgtggt
     9361 ggtgtgccaa gtaaaacaga aaaatattat tcctacaatt atgccaacat acatttcatt
     9421 gtattagaca gttatggagc acgttcagat aaagaaagtg aaatgtataa atggcttaat
     9481 gctgatttga aagctaataa tgcacgctgg actatttgtt attttcatca tccgccttac
     9541 agtcgcggaa cacacaattc tgataagccc ggagagtcaa ccgatatacg tcaaaacatt
     9601 attccattac ttgaaaagta taaagttgat ttggttatga gtggtcatgc acatgattat
     9661 gaacgcacat ttttgttaca tggacattat ggcaaacaag ccacattaac acaagatatg
     9721 atagttgata agagtgatgg aaattcacca tactatctta aatcggcacc cgcttataaa
     9781 gggaccgttt atgtagtgaa tggtgttggt gggcagggag gcaatgtaaa aactcaaggc
     9841 gactggccgc ataaagcaat gtgttcttat tccaaacagc attacggatc catgataatt
     9901 gatgtcaatg ccgatacctt gtcagcagta ttcttaacct ctacagatac tatatttgac
     9961 cggtttaaaa tagtaaaatc cgataatcag aaatttaaga agaatcaaaa aacaggatac
    10021 ctcaatttac aaaatcaaaa tataaataaa ttcaacgaag caaagagagg aaaaacggga
    10081 aaacagtttt cagacgcagg acggattagc gaagtagaac aaaaatcttt taccacgact
    10141 tatcgtccgt cagtaaatta caaaatgcag caatattgaa tttgtttaat tcattgtttc
    10201 cttcctgaac aacatatagt ttttctttct gggtaagatg ccagcttaat gaaacacgat
    10261 cttctttttg attcatctgc aaatcaataa agcgaatttg tctgccataa attgttttca
    10321 ggtattcaga aaaactatcg tttacataat cctgcattac aataaaatta ctgctcagat
    10381 ttttaaaagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgaactgctg
    10501 aaaattgaga aacaaatcac taatgataaa actaaatgcc gtagcattta atacatcttt
    10561 ggaaatgttc ttcagatagg tttccttatt ttccagtatt ttctgtgagc ctttatttcg
    10621 caaatacaat tcacgataat aagaagctcc tatcaatcct cctgatgatc cacttataaa
    10681 ttgagtatat ctaagcaact gtccattcag tgcactatcc aactcaagca ttgcattaaa
    10741 ggtgaataaa gcagaacgaa caccgccacc actaacgctc agaacaacaa gttttggttt
    10801 ttcaactccg gcatcattcc atttcttctt ccagttatta aggctcacca aacctgtttc
    10861 gtaatctttc tgcaaacggt agtgacttag aatttcctcc attttcggaa tggagtactc
    10921 cggttgtgcg ccatcataat caagaccgta tgctttgttt ttgaaattaa aaacttcaag
    10981 accggataaa aagtttatta aaataaacaa accgactaat gcagtatatg cccagccacg
    11041 aagccagtaa cgaaaaacac cgccaatcat gataatcatg gtaaaaagca acaatatgct
    11101 accccctgcc ggaattctga aaacactgta gtcacgaaat aatcctagta aaataaacag
    11161 aaggaatata atcatttcaa gcacggctgc attaatgtgg ttttgcctga acaccgatgc
    11221 aagcataaaa gatttgtagt gcgatgtact tcggactaaa cggagtttaa acgggaatgt
    11281 taagtaggtt tcaactttcc atccgcgtct gcgctgacgt ttaagtttat tatctcgaat
    11341 tggtgaaacg aattttacat tatcatggtc gccatcgtgt gtagcaacac caaaaagttt
    11401 ataaatatcc ttattggtat gctgaaaata ttttaatgtt gagaagataa caaagccaac
    11461 tcccattaaa agagctaata cctgaatggt gacaatactt attgggagca actgattttt
    11521 taactgaaaa tagtagatac ctccgatata agttagaata aacataacag gaagcacagc
    11581 attgttgtag cagtatttct caaacgtctt atacaaacat gcaagaaatg ggaatctgaa
    11641 gctattgagc atataacttg aaatattata tgccataata aaaacaccca gacaaattcc
    11701 aataataaaa aaagcaagcc aacttacttt acccatgtat tccgggtcaa gaaaaagata
    11761 gggaatacca aagcggttac caaaattttg cgttacacag gcaaacaaaa tcaaccaata
    11821 gactaacagc agttggctct tctttacatg cattattacc aactgaacag gaaaagaaag
    11881 ccagaaaatt tttagtctgt ctttaatatt cattactgca tcttcaacta tattatacgc
    11941 ttcacaatta atttggttct aaagtcctga aattttacga tttactacat ccagaatatt
    12001 tttttcattg gcaattgctt tgttttccat ctcagcagct tgttttaaga cagtttcaac
    12061 aaaagtttta tccgaatatg cagcagcatt gatccgtaca ttaagacctg cccctaaaac
    12121 agcagttctt gcacacattg ctcctacacc tgcatctgaa acagaatttg gattaccatt
    12181 agttgccatt gcctcaatga gctccattga ctgcagcgaa attttcatga ctgataaggg
    12241 tacttcaatt gcataacgtg tggcttcagc aactgcttgt ttcctgtctt ccttctcctg
    12301 atcagtagtt tttggtaact taaatgcatc cattatttta ttaaatgcca ctgtatcttc
    12361 atcaaccaaa tgaagcaatt gctttttaat tttctgccct ttatcagccc agtcagaaaa
    12421 ttcattccat cgttcttccc atcctttttt ggacgcagaa agattggcta ccatggttgc
    12481 aagtgcaaca cccaatgaac cacaatatgc agcaatagat cctccacccg gagcaggaga
    12541 ttcgctggct gtttcatcaa caaaagcatt caaacgcata tttgccagaa cagctttgct
    12601 ttcactctcc atcacatatt caataattct tttttccgga gtgaatggcg acaactcatc
    12661 aagccccatt gactttactg caatatgaat cagttcagca tcgctaacac ctgatgacaa
    12721 tcgctgcttg ttcagaaaat attttcctgc atcgagcatt gcctgtagtg gcaccaatcc
    12781 cactaactca cttcctgtgg cacggtagcc acgctcatag gcactgcggg cgctttcttc
    12841 aaaaacaata tgcagcggag tttctctgaa ctttgtaata ttaatcgaga cctgtgcaat
    12901 accatattct tcaatatacc atcctattgc tttaacgcct ttaagcttac ccggtattct
    12961 gatttcatta cctgcatcat ctaatatttt ttttccttta ctatcagttt tcacgcggcc
    13021 tgcttcacga atatcgaaag caacagagtt tgcacgttta accgatttgg tattcagatt
    13081 aacattatag gcaattaaaa aatcacgagc accaattact gtagcacctg aatttgcagg
    13141 caattctgca ggaccaaaat caggtttcca ttctttttgt tttatctttc taaagaagcc
    13201 ttcatattca cctgctctga tgattgaaag attacttctc tccttatttt cttgtgcttc
    13261 ttcatataaa taaaccggaa tgtgaagttc attgccaaca cgagcagcca gcttctttga
    13321 ccactctacc gtttcttgca ttgaaatatt ggctacagga ataaaaggac aaacatcggt
    13381 ggctcccatt cgtggatgtt cgcctttatg cttactcata tcaatgagtg tggctgccat
    13441 tgcaatacct ttaaatgctg cttcaactac tgctgcaggt tcacctacaa aagttacaac
    13501 tgtgcggttt gttgctttac ccggatcaac attcaataat tttattcctt ctaccgattc
    13561 aatactatcg gtaatttgtt tgataatagt caaatcattc ccttcgctga aattgggaac
    13621 acattcaatc aattgtttca tttaaatttg ttttaatctt tttgaaaaat acgtttgcta
    13681 agatcatcaa aattaccctg aaaaagattc cgcagatgaa caataaaaaa agttttaaag
    13741 taactgcgtg caaaagattg ctttaacagc ttaaaagaac ctcctcaggt ttaagtcaat
    13801 aaaaaagtta tcagactttg ttatttttaa aagtactgcg ggctttaata tagtcggcaa
    13861 ttaaatagcc aatgagttta ccggttttat tatccgtttt aatgtgtgaa agcaccggtg
    13921 ctgcttcggc aatatgcaaa taacaggcat ttagctttga ggctgcgcga ttaatatact
    13981 gccttgcctg aatggtgctg ataccgcttg atgttttagc acttgaagga atattctgaa
    14041 tggtatccat atcaatttca ataccacaga agttgtcttt aacatgttca atagctttat
    14101 caactgcttc tgtaaatgaa atattttctc tgataaaaat atcttcaaaa aaagacagcg
    14161 aaaacctttc aggtctgtcc atcataaact gcaacacatt acctgcatta tagctctcat
    14221 gcaatcctac aattgcatac ttgttgagaa atttatgctc atatgcatat cgaaatccat
    14281 ttccactgtg acgaccttcc ataattctga aatcggaatg tgcatcacaa tttatacagt
    14341 ttatactctc acttttaatg actccacttg tttgcagacc aatagcagca gccttaatat
    14401 taggatatgc gttattatgt cctccaccaa ttataatggc ttccttgccg gattgaatta
    14461 tcgcctttac aacctcgaaa actctttcat caatctgtcc aacaagttct cttgccttgc
    14521 ttattccttt ctcagttttg aaattaatgc catcggcagc aagcatcaga tcatcaaaat
    14581 taatatgacc gagaactaaa atattactgc catcaccaaa acaattactc tgcacattca
    14641 acaaactaaa caatgcaggc ttccaggcac tgtaagcacc accccgacct ccattagctc
    14701 tgattccaat atcttcaggc agacccaaca acacatattc tgcatcacac tgatttaaac
    14761 gttcaattag tgttgcatcg cttttattga gcacctgcac attctcgccc accttggttt
    14821 cattcttgcg ggttcgtgtt tcctgattaa tatcttcccg actgtaaaca acaagatgac
    14881 tcatacttac acaacttttt tgccttttac ataaacagat tcaatcacat tttcattgaa
    14941 tgagtagggt atgatataag gtgatgatat cgcttttgta ataaaaaaat ccgctctttt
    15001 accaacagaa atactacctg taagatgact tacattcatg gcataagcag cattgatggt
    15061 ggctgcatta aatgcttctt caggatttaa tttatactgt acacaaagaa gacttatcat
    15121 aaaattcata tcgcctgagg gcgaacttcc gggattaaaa tccgatgcaa ctgctaaagg
    15181 taagccggca tcaatcatct ttctggcagg aggaagtggt aagttcaaaa agaatgcagc
    15241 accgggtaat acaacaggca ttgtagtgct attttttaga gcagcaattt cttcgtcttc
    15301 tacaaattct aaatgatcaa cactaatagc attgcagttt acgcctgcct gaacaccacc
    15361 tgagcgactc atctgattgg catgtacctt gggtattaaa ccatattttg ctcctgcatt
    15421 tagaatatct tctgtttcct gctgtgaaaa ataattccgc tcacaaaaca catcacaata
    15481 atcagctagc ttttctgcag caactgccgg tatcatgtca tcaataatgt gcctgacata
    15541 gcctgcttta ttgtttttga acttttccgg gacggcatgt gcacctaaaa atgtagattt
    15601 tataatcagc gatgagcttt cttttattct ctttattatc ctaagcattt tcaattcgct
    15661 ttcaaggctt aagccatatc cgctctttat ttctattgct gtggttcctt tggctatcat
    15721 tgaatcaata cgttgcattg ccgaaagaaa caaatcttcc tctgatgccg cagcaagttt
    15781 ttttgatgaa ttaagaatgc ctccaccacg ttgtgcaata tcttcataag acaatccatt
    15841 gatacggtca acaaattctt cttcgcgcga agcagcataa ataagatgtg tatgcgaatc
    15901 gcaccagcca ggcattaaat atttcccatt tgcctgaacc ggttcataag cactatattc
    15961 ttctttcacc gactcccatt cagtcatagt accatagctc ttaataagac catcttttat
    16021 tactaaaaag gcatcaggca aaaacatgta gctgcgcaaa tcctgtccac gtttgattaa
    16081 tatatccttg tcaagaattc cacacagacc tttaatatta agtattacaa tcacaatttt
    16141 agaattagat tttgtttgag ataaaactat gatgaatatt tataggtttt tcttaaaata
    16201 ctctgtcatc atttcataca aatgtcccct cgactgagag atgccatgtg cctgattggg
    16261 ataataaaaa gtgtcgaatt gttttttatt cttaactaat gcaataacca tttctgcact
    16321 attctgaaaa tgtacattgt catcagttaa tccgtgaatg agtaaatatt tacctttcag
    16381 tttatcggca aaaaaaacag gtgagttatc atcataacct tttggatttt ctgccggcag
    16441 agacaaatat ctttctgtat aaattgaatc gtaatagcgc cagttgataa caggtgctac
    16501 ggcaactccg gttttaaaat aatcggcccc ttttgtcata agcaaagatg tcaggtaacc
    16561 accataactc cagccatgaa caccaatacg ggttgagtca acataagata gggtacgaag
    16621 atattttgca aattctatct gatcaatcgt ttcataatga cccatgtttt tataaatact
    16681 attggcaaat tcctttccac gtcccggtgt acctctgttg tcaaccgata caacaataaa
    16741 accgttttgc gcaagcatct gatgccagag aaaatttgtt ccaccccatt ggttaaggac
    16801 tgtctgtctg cccggtcctc catataaata aacaataaca gggtactttt tattctcatt
    16861 ggctttaaaa cccggtggcg ttatcatcca tgcattcagg ctgatatttt cagagttagt
    16921 gaatttaaaa aattcaggtt tagaaattgt ataagattca agattcttta ccactctttc
    16981 attattttcc aacaccctta tttttttacc tctgctgtcc tgaacataac agatataggg
    17041 tttgtcaatt gtgctatgtg taattactga atacttaaaa ttgctgctga aagatgctga
    17101 tgcagtaccg ttggcaggcg acaacaattt cttcttctga ttcaggtcaa ctgaataaac
    17161 aactctttcc atgggtgttg ggtctgcaac ctgataaaaa cagttttttg ttttctcatc
    17221 atagccataa aaagttatta catctccacc attggtcagc atcttctcag agccatcact
    17281 caggcttcta agataaatct gattgtaacc gcttttatca ctgctgtaaa taaattgtgt
    17341 tccattattc aagaaaacta agttatcggt aatctcaata aaagtgttac tattttcatt
    17401 cattaataaa gtggtcttgc ccgaaacggc attacacaat aaataatcca gattattctg
    17461 atgtcggttc attctcaata cacataactg tcctactttt tctgtccatt ttattctcgg
    17521 aatatactga tctttttcat ctcctgtttg cattctaaca cttctgcctg aagacaaatc
    17581 gtaaacataa atatcaacaa cagaattctc ttcaccggct ttaggatact tatatttctc
    17641 ttctttcgga taaagtgagt cgtaataagt aagtgtaaat tctttaacat tgctttcatc
    17701 aaacctgtag tatgctaaat actttccatc gtttgaccat tgatatccct gactgaatga
    17761 aaactcttct tcgtaaaccc agtctaatgc accattgatg ataaaatttt tctttccatc
    17821 gttagtaacc atcttttcac ttaaatcgta aagatttttc agatatagat tattatcacg
    17881 aacaaaggct accatgcttc ctgttggatt aaattgagca tacatttgtt ttcctttttc
    17941 tgaaacagct gttaactttc tgcttttgaa atcaaaaaca tagaagtttg ctttggttga
    18001 atgtctgtaa attgcctctg atgctgtggt cagcaaaact tttttttcgt cattactaaa
    18061 cgaataatca gaatacctga agtctttaat tgatgcttgt aattcatcaa ttttaataag
    18121 cgtgtctatt acatttcctg ttttaaaact gtaacgaatc aggtttcctg ccctgtcagt
    18181 gtttgaatag gtgtcgccac tgttcatgct ttttaaggat gcaacctgtc tggccgcaaa
    18241 ctgtgatgaa ccccagatgt cggcataagt aaaattcttt tgtgcataca gatttaaagt
    18301 agctaaagca aagaagaaaa gagaaaatat ttttttttcc attacataaa atttaaagtt
    18361 caaacctaca aagaatacag aaattagaag ttacaatgaa taaagaagag aataaacttt
    18421 attagttatt aaggtttggg tataaaaaat gcggtcacag cctgattttc aggctgtgac
    18481 cgcaaactta aaatatggac tcttaaataa gtttttctat agttgccttg cttgagtaag
    18541 cttgcttacg agatgctgat tgtgcggctg ctttttcagc ttcaatctca tcataaagct
    18601 tgaactcatt caactgatct tctccaataa ttttagagag acctgaaagc attgagccta
    18661 tcttctgaat gctctgcatt tctgcttttg ataatgctat tactgccata gtattaaaat
    18721 tttgatgacc aaatgtagat attaatttat taaaaccaaa tatttttagc taaaagtttt
    18781 aaacagaggg gttaaattac cctaatacgt ggcaaatcac tttgtttctt catcaaaact
    18841 aatgtcagta taggggtttg aactaccacc ggaactgtta ttgtcatgat ctgatttccc
    18901 tgcattgcca tttttgatgt gcgacactgc ctcattcagg ccttcgataa atttctcaaa
    18961 atcttcttta taaaggaaga ttttatgttt ttcataaaaa ggcttgtcat cgcccatgtt
    19021 aaacttcttt ttgctttctg taatagtaac aaaatagtcg ttagcccggg ttgacttaac
    19081 atcgaaaaaa taggttcttt ttcctgcctt aactgcttta gagaagatta cctctcttga
    19141 gtttccatta ttttctgaat cgtgcatata atgctctttc ggtttatacc tgcacaaaac
    19201 aatacaaaaa aacttaatcc tcaaattttt tgtgaaaatt cttgtaatta actcaaacag
    19261 agggcatcca atataggatt ttatctgtgt aattcttcta agtatgactg gttattccct
    19321 tcctacttaa aatagttttc atgaagaaaa ttatagaggt taaagtcctt tgaatcggta
    19381 gccgtctgaa gtttgatttt taaagactga tagtcaaaat ctcgcatttt attgctgatg
    19441 ataagacgaa tagatttttc ataaaacaaa ttcaatttat ctgaatgctt tcttgtttta
    19501 tcaaccaaac attctgtcaa taaatctatt cctgtacctt ctgttgccac ggttttaaaa
    19561 atcggagaat agcgtgtctg atttcgcaat cgtaacatat tctgaatgtg ttttacaagc
    19621 acatcagcct gctctttgtc tgccttatta ataacaaaaa catcagcaat ttccataata
    19681 ccggctttca atgcctgaat gtcatcaccg gcacctggat ttaaaaccaa tactgtagtg
    19741 tcggctaatg cagcaatttc aacttcactc tgccctactc caacggtttc tataaaaata
    19801 aaatcgaatg cacatgaacg caacacatca gtaatctcaa tagttctggc agacagccct
    19861 ccaagatttc ctctcgtagc caacgacctg ataaacacat tttgatgcaa aaagtaatca
    19921 ttcattctgt aacgatcacc taaaatactt ccacttgtaa aaggtgatgt tgggtcaact
    19981 gcgagaattg cgatgcgcca cttgtcagcc attttagcaa gcaatgcatt gagcagtgaa
    20041 cttttccctg caccgggcgg accggtaaat ccaatcacag gtatgtcata atttatcttc
    20101 agatgacgta gtaaatccat tgcaccatca gcagcattct caacctgaga aataactttt
    20161 gccagcgaac gataattgcc ctgacttatt tcggcttgat ttaaattaaa tgacatgctg
    20221 caaagataga attgcattta aatgtgagta gccattttgc ggtgagataa tgttattttt
    20281 gcatcatatt tatgcttaca tggtaaaaat ttcaggagtt ataatcactt ttaacgaaga
    20341 gaaaaagatt gaacaatgtc tgaaaagtct tttacctgtt tgtgatgaga ttgtagtatt
    20401 ggattcgctg tcaactgata aaacagaaga aatatgcagt cgctatgaag tccggtttat
    20461 caaacaaaag tttttagggt acattgaaca aaaaaaccat gctttgactt ttgcatcaaa
    20521 taatttagtg ctttctttag atgcagatga atgtctttcc gaatctttac agaagtcaat
    20581 tgagcaaatt aaaaataatc aaactacaac agcatacagc atgaacaggc ttacccgata
    20641 ttgtgataag tggatctatc atagtggttg gtatcctgat gtcaaggtgc gcttgtttga
    20701 taaaacaaaa gcacaatggg gaggaacaaa tccgcatgac aaaattattc tgtcagaaag
    20761 aacttccgtt caacatttaa aaggcgactt acttcattac agtattgaca gtatagagca
    20821 acacatacaa caggcaacaa agtttggcat tattgctgcc aaggcaaaat ttgctaaagg
    20881 tcaatcttca aatggattca acacgctgtt acatacggca ggaagatttt tgaaaaccta
    20941 tattttaaaa gccggatttt tagatgggta ctacggctgg ctcattgcta aatattctgc
    21001 caaaagtgtt tatctgcgct attccttatt ggaacctcgc aaccacgaaa aactaaatag
    21061 tctttgatga aggtattaca catatctaca gcaacaggtt ggcgtggtgg cgagcagcaa
    21121 gcagcatatt taataaatca acttggtaat ctgcaaacag catgtttctt attatgtcca
    21181 aaagatgctg caatcatgca acgattaaat aattctaact gtaagataga agtctttcaa
    21241 aaaggtaaag cacttaatct atctttagca agaaagataa aaacactttg taaagaaaat
    21301 caaattgacc ttatacatac acatgactca catgcacata cagcagcatt tttatcatca
    21361 tggctatttg gcaataaatg cccggttgta gtaagccgca gagtagattt tgccgtttct
    21421 tcctccctgt tgtctcattt aaaatacaat cactcaagta ttaaaaaaat tatttgtgtg
    21481 tctgttgcca tcaggcaaat tacagcacct gctattaaaa gaaaaaaaat tttagaagtt
    21541 gttcattcag gagttgacac agataaattt attttcggta aaacaaaact acatcaggaa
    21601 taccggttgc caaaggaaac atttcttgtt ggcaatgtgt cggccttagc tgatcataaa
    21661 gacttattta catttattga tacggcagaa attattttac aacaagctca acacattcat
    21721 ttctttctga ttggtgatgg ttctgaaatg aatgcactta aaacaagagt tacagaaaaa
    21781 aaacttacag ctaacataca ttttacaggt ttcagaaatg atgttaatga attacttcct
    21841 gaactcaatc tttttctgat gacatcgaaa acagaaggtt taggaacaac aattttagat
    21901 gcctttgcag ctaaagtgcc tgtggtggca actaacgcag gtggcattcc ggaaattgta
    21961 cggcatgaac aaaccggatt aatcgcacca attggcaatg cacaacaact tgccaaactt
    22021 gtattggaag taatgaatga cagtaatctt gcaaatacac ttgcaaccaa tgcacagcag
    22081 ctggtagctg actttacttt caggcgaact gcagagaaaa caaaagctgt ctatactcag
    22141 gttttacaag aggaaaatca gaaggtataa tggccgtaaa aaacgaacat ataaaagcca
    22201 ttctagaagg acttccaaat gaacctgggg tttatcagtt tttcgataaa aatgaaaaga
    22261 taatttacgt tgggaaagca aaaaatctga agaaaagggt ttacagttat ttttctaaag
    22321 acatttttga aagtgggaaa acagctatta tggtaaggca gatttctgac ttgcgtttta
    22381 tcattacaga aactgaatta gatgcgctgt tactcgaaaa caacctgatc aaaaaacatc
    22441 aaccacgcta caacattctc cttaaagacg ataaaacata cccatggatt tgtattaaaa
    22501 atgagccttt tccgagaata ttctatacaa gaagaaaaat ccgtgatggt tcacagtact
    22561 ttggtccgta tgcttctgtg aaaatgatta acattttatt agacatgatt cacagacttt
    22621 ataaactcag aaattgcaat ctaaatctta ctcctgaaaa tattcagaat aaaaaattca
    22681 gagtttgttt agagtttcat gtaggcaatt gtttgggtcc atgcgaaggc cgtcaatctg
    22741 cagaagacta tcagcaaggc attgataaca tcagacatat attaaaaggc aacatcaatc
    22801 aggtgattcg catattgcag gatgaaatgc agacagcttc gcaaaatctt gaatttgaaa
    22861 aagcacagca gataaaaagt aaactcgaca ctttagaaca gttccgaagt aaatctgtca
    22921 ttgttagtcc tgttattaat aatgttgatg tagtgacaat tgtgtctgat gaaaattatg
    22981 ggtttgccaa ttatatgaaa gtgattaacg gagcaatttt gcaatcgcaa acaattgagc
    23041 tgaagaaaaa actttcagaa acagataccg acctgataac ttttgcattg acagaattac
    23101 gtcagcgatt tgaaagcgat gcaacagaaa tattggtaaa tgtaatgccc gacacctatc
    23161 ctgccgatgt agaggtgagt gttcccaaaa taggagaaaa aaaacatctg ctcagtctct
    23221 ccgaaaaaaa tgcacgttac tatcagttgg aaaaattagc gcagtatgac aaactaaatc
    23281 ctgaacataa aacagacaga ttgatggaga aaatgaaagc agatttaagg atgatggagc
    23341 aaccgcgaag gattgaatgt tttgataatt caaatttcca gggtacaaat gccgttgcag
    23401 ccatgagtgt atttatcaat ggtaaggcag caaaaaaaga atacagacac tttaatatta
    23461 aaacagttga agggcctgat gattttgcat ctatgcagga agtcatctac aggcgctaca
    23521 aacgtgtttt ggaagaaaaa caacctttac cccaactaat catcattgat ggtggcaagg
    23581 gacaacttaa tgctgcactg accggacttg aaaaattagg gttaaataaa aagattcaaa
    23641 ccattggtat tgctaaaaaa ttagaggaga tatattatcc cggagattct gtacctatgt
    23701 atcttgacaa aaaaagtgag accttaaaaa tcattcagca actaagagat gaagtacacc
    23761 gtttcggaat aacccatcac agaaataaaa gaagtcgcaa ttttctgcaa tcagaaatat
    23821 tgcaaattcc cggtataggt aatgcaactt ttgagaaact gattatccaa tttaaaagcg
    23881 tcaaaaaaat caaagaagcc actgcagctc aaattgcaga tgtcatagga aaacaaaaag
    23941 ctgcaatagt tgtaaattat tttaactcag aaaaacaaca agctgatgaa gtcaactaaa
    24001 atattttatc tgttagtcgt ttatgtgctg ctgcaattgg gttggtggtc gtatctctta
    24061 gtaaaattaa actctgagtt aggcaacgaa aaattgcgtt acttagaaac gctgcctcaa
    24121 actgaagcaa ccatacttca ggaaaatgcc attcgcaaaa agctaaaaga aaaatatctg
    24181 atggtggctg gtgaaggttt ggtgttttta tcgcttctcg gtattggaat tttctatgca
    24241 cgcaggtcgt ttaagaaaga atttcagatg gcattgcagc aaaaaaactt tatgatgagt
    24301 attacgcatg agtttaaatc gcccattgca ggtatccgac taagtttaga aacattgtta
    24361 agacgtgatg tagaaaaaca acaacgcgat aaaattcttg gccgcgcctt acttgaaaca
    24421 gacagaataa attttttagt tgaaaatatt cttacagcta cgcgcattga ggctgcaaat
    24481 atggactttc agtttgctga tattaattta tcagaaacac tttcagacct gatgactata
    24541 cttcaacaat ctgcaaacag tcataatttt aaattgaaaa ttgaaaacga tattcatgtt
    24601 acagctgata aaaatgctat acattcactg atacttaacc ttatagaaaa tgcacagaaa
    24661 tatactcctg caggctccga cattgaagta aagttatttc agattgacaa agatgtattt
    24721 atgcagattt gtgataacgg cccaggtatt gctgatattg agaaaagaaa aatttttgaa
    24781 aaattctatc ggataggtaa cgaagaaaca cgcacaacaa aaggtacagg attagggctt
    24841 tacatctgtc gctttattgt tttaaaacat aaaggccgga tagctgttca ggataattat
    24901 ccgcaaggct gtatttttga agtcgtaatt ccatcattaa acaataaaat ataaatatga
    24961 acaagaaaaa agttgtgctg attattatgg atggctgggg tgaaggccgt aaagacaaaa
    25021 gcaatgcagt ttatgaggca aatactccgt ttatagattc ttgttatgat aaatatccgc
    25081 atagtgagtt acttacttcg ggacttaatg ttggtcttcc tgacgggcag atgggaaaca
    25141 gcgaagttgg gcaccttaac attggagcag gcagaatagt ttatcaggaa ttagcactca
    25201 tcaacaaagc tgttgaagaa aaaacgttgc atagcaatac tgtaattctc acctgtcttg
    25261 actatgtaaa acgtaccgga aaaaaattac accttatcgg actggtgagt aatggtggtg
    25321 tacattcaca catcaaccat cttaaagcgc tttgtgacat tgctaaagac aatgatgtaa
    25381 aagatgtttt tattcatgca tttactgatg gtcgcgattg tgatccaaaa agcggtttag
    25441 gttttataaa ggaactagaa aatcatttaa aaatatcttc gggaaaaatt gcctctgttg
    25501 tagggcgata ttatgcaatg gatagagata aacgctggga acgtataaaa gaagcatacg
    25561 atttaatggt taacggaatc ggtgtgcact tcggcaatgc cgttagcggc atagaggcat
    25621 cttacaaaaa caatgtcacc gatgaattta taaagcccat ttgcattaca gaaaacaaca
    25681 aacccgttgc aaccatacaa gatggtgatg cagttatatg ttttaatttc cgtactgacc
    25741 gctgccgcga gataactaca gcattgacac agcaagactt ccatgagcag aatatgcata
    25801 agctaaatct ggagtatgtt accatgactg tgtatgatga taaattcaca ggtgttaaag
    25861 caattttttc caaagacaac ttagaaaaca cattgggcga agttttgcag aatgctaata
    25921 agtcacagat aagaactgct gaaactgaaa aatatccgca tgtaactttc tttttttccg
    25981 gaggacgcga gaatgaattc aaaggagaaa aacgtatctt gattccgtca cccaaggtgg
    26041 ccacttacga ccttcaaccg gagatgagtg cattgctatt aaaagatgca ttggtgaatg
    26101 agctaaaaaa aggcgaaaca gattttgtct gcatcaactt tgccaatgca gatatggttg
    26161 gtcatacggg tgtatttacg gctgccgtaa aagcagtaga aacagtagat gcctgtgtaa
    26221 acgaaattgt gcaatgtggt ctggaacagg gttattcatt tttaatcaca gcagatcatg
    26281 gtaatgccga ttatatgatt aacgatgatg gctcaccaaa tactgctcat acaaccaatc
    26341 ctgttccact gtttcttatc cagccagagc atgagttcaa tttaaaaaac ggtatcctgg
    26401 ccgaccttgc tcctactatt ctgcaactga tgcaagtaca acaacccgct gaaatgacag
    26461 caaaatcgtt gctttcatga acaacattac ttaacatatt gctgtgtaaa acataaatat
    26521 aacagcgggc gttttatagt ggtaggttca ggcaagaaac tacttttgca ccaatcggga
    26581 gtgagcatga aaaaaattac cctgttactt ttaatgttgt ctgtaaatca actttacggt
    26641 caaagccgca ccttcttttc tctgtcagga ggtattcttt attacaatgg cgatcttaac
    26701 gataaaagcc ctgttccatc aacaaaaatt ttcagaaact atggtaatgc agccatcggt
    26761 attcatctta caaggcatgt caatttacag ctaaactact atcatggtag tattgaaggt
    26821 gcagactctt taacaaagga acgcgacaac aaacagcgca atctaagttt tgaaacccga
    26881 attgacgaaa tatcttttca actcagtgct agtttattct ccaaccgcac aaaaaaactt
    26941 gccatacctt atgtatttgc aggaggtggt ttgtttttct tcaatcccag agcacgttat
    27001 tatggtagca atcctgagta ttatggaaaa ttagtttcat tacaaccgtt aggcacagag
    27061 ggacaataca tcactgaagg tgattatcct aaaccttaca aactatatca ggctgtattt
    27121 cctatcggca ttggcacgta tatcagaatc aatcctaaat ggcgactgaa agtagagttc
    27181 tctgagcatt ttactttgac agattactta gatgatgcca gtggaaaata tccaaacatg
    27241 gaagcattat cactgacacc taaaggtgaa ttggcttctt actttgctga cagacggcta
    27301 aacagaacac aggatcctac gggacgaaac cgctccaatg caacaaaaaa cgacaactat
    27361 attcactttg gtattggttt agtttacaac ccacaggctc aacatcctac ccgatacaga
    27421 ggtcccggat tctggaaaaa acttttcagc ggacgcaaag gctggtgggg caatgccggc
    27481 agtggccttt aaaaacaatc atcatatcgt catcaactga tttaactttt catgttgtgg
    27541 aatgttatca ttctaaataa catgtatatg aaaattttag ttccggttga ctttaccgat
    27601 attactgaaa aaggattcaa atatgcattg tccctacctt ttgcaaatga aataatcctt
    27661 tttcacgctg ttgatgacat aaaaaagtct aaagaagcac aacagcaact cagcaacata
    27721 accaacaaat attccgcgcc tgcacatctg actgttcaga gcaaagtaaa acaaggtgcc
    27781 atttttgaaa taataggtaa tgtttcaaaa gaagttaatg ccgatttagt tgtgatggct
    27841 acacatggta ttaaaggact tcagcatttt ttaggcagcc atgccatgaa gctgataaca
    27901 cattcaaaaa ctccatttat tgttgttcaa cagaaagatt tcagtccact gaaaaaaatg
    27961 cttgtgccgg ttgatttcac taaagaagta aaacaagagt tacctgctgt aattcaatat
    28021 gcacagctat ttaatgcaag cgtgctttta cttaaacaaa ataatcacga cccttacatt
    28081 caaaaaagaa gtgacctcaa caccggatac tttgaagacg aactcgataa tgcaaaaatt
    28141 aaatatgaat tgattgagca gacattttct ttagatgata aatatgaagt gctaaccaaa
    28201 caagctgctg ctcataatgc cgacattatt gttactacta ttgagcctga cctggatgtt
    28261 gccgactatg taattggtgt tgaagagcaa aaaattgtag ccaacgatct tcaactgcct
    28321 gttttatgtg taaacattag gcattttact gttgccggtg atcagttcag cgtttctgta
    28381 cagcaataaa gtttaagtac aattctttca acttcgttac tttgacattt gccaaataaa
    28441 aaacaatttt gaccaaaagg ttgccccttc tgtcagtata aaactgcaaa ttggtttaac
    28501 aattaagtca acaatcgtta aacaaagaag tatgtcattg gtaacctgca aagaattcaa
    28561 taaactctca catgatgaac aaacctggta tatatggaat ggagcaatcc atcttcatat
    28621 aagagaagtg tatggctaca gagtaaatct gttttatttc aatgaatttt acattgaact
    28681 ttggtacagc atcaatgaga aaagtattaa aaagattaag tcctttgaca attctgcact
    28741 gttggcacct tatttagaaa caattaaaat tgattccttg ttgaatattg attcaggttt
    28801 tgaataatat tactgtccgc taaaaacgta ttgcagaata ttagcgccca tctgcaaagc
    28861 tttttgtcgc atttctggcg ggtcgttatg tacttctggg tcttcccatc catcgccaat
    28921 atcactttca tattcataaa aacagactaa cctaccctga taaatcagtc caaagccttg
    28981 tggcgccttc ccattgtgtt catgaatttt aggcagtcca ttcggaaaat taaaacgctg
    29041 atgatagata ggatgagtga atggtaattc tacgaagtca agttccggaa agactttttt
    29101 catttcagga cggacaaact tatccatgtc ataactgtcg ttgatattta aaaatccacc
    29161 ggcaatcaga tattttctaa gattttcggc ttcctgattg ctgaacacaa aacgaccatt
    29221 tcctgtcatg tgcacaaacg gataattaaa caagtcaggg ctgccgggtt caacaactgc
    29281 ctcttcggga ctgatgttgg tattaatatt ctgattgcag aaaactgcaa gattatgtaa
    29341 tgctgtaggg cttacgtacc aattaccgcc acctgcatat ttcagtttgg caagcttaag
    29401 tgtaaaatca ggtgctttaa atgaagaaag cagcaatacc atcacggcca tgattttcaa
    29461 cccacaaagt ttacggaaat gcttcatttg accccaaaaa tatgtttaat caatgacaca
    29521 atcttatttg caccttaatt ttatatgaat aattttgtca gtccaaaaaa tcaaatctat
    29581 ttaattatga ctttgcgaat tttactcgtt gcaatttttg caccgttgat gcttaatgca
    29641 gcccatgatc acaacggttt cgaccgtaat gaagatccca atcttaaagt gtttgttaaa
    29701 aacagaaaac gtttacctga tgtaaatcgt caagaaagtc ttgcacaaca aaaatcatgg
    29761 caacagtttt cagaacaaaa caaaggatgg tctgtagttt ttagcgaaga gacaggtctt
    29821 ccacacaatg cattcggaaa accaatttca gtaggattac ctttagatgc gcaatctaca
    29881 gcatggtcgt ttatcagcac taagctcaat gcttttggtc tgccgctgaa cgatttatac
    29941 ttccgcaata cagcacaaaa caaaaaatac ttttatgtaa actacattca gcgttataac
    30001 ggacttgaag tgttgtggtc taaggtgcaa attaaaatca ccaaagacgg caaggtgaat
    30061 cagtttattt gtgatgctta taaagatatt gctgtaaata ttgctcctgc acttagttcg
    30121 cagacagcca ctgcagcggc aacaaatggt ttaaatctta ccatcaacgg cacttcagta
    30181 aataatgacc ttaaaatttt acccgttccg gatgagggaa aaaaccacta tcatttggta
    30241 tatgaagtaa cagtcaaagc taccgatgca gaaagttttc cgagagaata ctatacgctt
    30301 gttgatgcac ataatggtga agtactttat cgtcataata aaataaatta ttttgctgca
    30361 aatacagatg tcaatgtaac tgcaactgtt tatcctacac aaccctattt gcccacagca
    30421 gtagagccat tagccaacat gaatgttacc atctccggtg taccttttct gactgatgca
    30481 aatggttatt taggacttcc aaacacatca cctgtaactg ctactttcag attagaaggt
    30541 agctgggcta atgtaaaaac aggtgctaca acgccacaat ttacagcaac tttaaatccc
    30601 ggtgccaata atattacttt cagcgctgcc aacatcaaag aactttctgc atacaattcg
    30661 gtaaatgaaa ttcatgatta ttatgtaaca aaactacttg gatcaggtgc tgaagctatt
    30721 atggattttc agatgcagac tatagttgat gttgccggaa attgtaatgc attttatgat
    30781 ggcaatttaa atttttatgc agcaggtggg caatgtaatg ctacttcgat tataaacgat
    30841 gtagtttatc acgaatatgg tcatggtatt aattatgatt tatactccta ctatggcggc
    30901 atattcagca atggtgctct tggtgaaggt tatgccgaca cctgggcaaa cggtttaacc
    30961 gaagatccta ttttgggaat aggattttat tccaacagta ccacaaactt tgtaaggcgc
    31021 tatgatatag gcagaaaagt ttatcctgat gacattatgg gcgaagtaca tgccgatggt
    31081 gaaattattg ccggtgcatg gtgggatgtt ggtgtaaact ttaacaatgt gcaacaacgt
    31141 caggatttat atgtacagac ttttgcagca actattgatt atcccgatgg acaagaagga
    31201 caactctaca ccgatatttt agttgaagcc ttaaccattg acgataatga cggtgacctt
    31261 accaatggta cacccaacta ttgtgcaatc acttctgcct ttggcttaca tggcatcaac
    31321 atgtttgttg ttggtaaact tattactcat aatagtgtac tcagcgctgc cggtcaacag
    31381 agtataaacc tgaatgtttc cggttcatca acttttgcca atactacggg aacaagcatc
    31441 aacggatatt acagtctgaa ccattctgcc acctggaacc ctataagctt tacttataat
    31501 ggctctatct ttcagggcaa tattcctgct caacctaatg gcaccctgat ttcttattat
    31561 cttgatgcta ccgatcaatg cggcacacac tttagtacta ccccatctaa tgttacagac
    31621 accaacccta atgttcctta ttatattttg gtagggtaca acttagtgga taatgactat
    31681 ttcgatgccg gcaacagtca gggttggaca ttgggtatgc ccggtgacaa tgccacaaca
    31741 ggacagtgga taattgcatc tccggtaggg tctctgctta atcccggtgg atcgcctcct
    31801 gtgtatgtac agcctcctgt agatcataca agcaccgcta acaacctttg cgcagtgaca
    31861 ggaaatgcat cgcctaacga tggcgcagga acaaatgaca ttgatgacgg aaaaacaaca
    31921 ttactctcac ccaactttga tctttcggct tattccaatc ctgccatttc gtactggcgc
    31981 tggtacacca acgatcaggg ggcagatccg ggcaaagatt actggcaggt ttctgttagt
    32041 aatgatggcg gaacaacttt tgttcctctt gaaaattgta tagttgccga ccatagctgg
    32101 agacgttttg cttttcgtgt agccgattat ctgacaccta ctgcaaacat gcgactaaaa
    32161 tttattgctg aagatgccaa cgatggcagc cttgttgaag cattgcttga cgatattgaa
    32221 atatggagtg ccgagcctgt aggcattgct gaaacaccaa tattatcatg gtatgcatat
    32281 cctaatcctg ccaatgagca acttaatttc ggatggaaag gtaatggtgc aaccgttgat
    32341 gttaaattat tcaacacagt tggacaaata gtatatcagc atcaattttc aggtaacact
    32401 gtttctgaaa ctgttagtct taaaggaatg gcggaaggta tttacacctt acagatgtca
    32461 ggagaaaata tatttaagtc gcagaaaata tctgtgatac attaaacctg taaccaaatc
    32521 aaaagccgct gtgttgtaaa acatggcggc ttttttcatt gctaagcaat tactatttgt
    32581 tgaatgatgg ccttgcctga tgcagtgcag gagtgttttc agttatctga accaagttgt
    32641 tgcctttaaa tgcatctctt aattgctgca caaaagagtc ttttgatttt ttatcctgac
    32701 caagaagttt aaaacaaaaa tctacttctc cttctctacc ccaggcaatt tcttctcttg
    32761 caataggcat cccggcatca gtctgatatt gttgcacaac ctgatctaat ttttcttttg
    32821 ctgatgcatc tgtacctgca ccaatactga tgaatgaaac aattacgcga tacatactat
    32881 caggcaaatc tacacgcggg tttgttgctg caggctcctg tttgggcgtt tccgcagttg
    32941 tgtttttatt tgttttacac gatccggcca tggcaactac cagcaccaag gcacaacaat
    33001 tcatcctgtt aagcatatag ttcatcgtat tttgtttttg cataattaaa aaaataattg
    33061 aagttaagca cttctccggt cacattttta catagctcat ctgccaatat cgttcttccg
    33121 tgacgatgga tattacttct taaccacatc agcagcggca tggtgtcacc gtacagaata
    33181 tgatgcatca atgcaggaat ttctttattt gcctgatgaa aaaactgtga tgcatagaaa
    33241 cttcccaagg agtaagtcgg gaaataacca aagctgccat gactccagtg aacatcctgc
    33301 aacacaccct ttgagtctga tggcacatca atgttcagat aagatttata cttagcattc
    33361 cacacctctg gaagttgtgc agcctgcagt gttcctgata aaatatcctt ctcaatctca
    33421 aaacgaatta atacatgcag atgataagtt agctcatcgg cattggttct gatgagtgat
    33481 ggtttgatac ggtttattgc cttgtaaaag ctcctttcgc tcacatcatg caactgctga
    33541 ggaaaaatat tttttagtgt tttgaaatta taattccaga aatattgcga tttgccaaca
    33601 tgattttccc acaaacgcga ttgcgactca tgaatactca ggctgagata ctcacccgat
    33661 ggcaatccat aatcatctgc aggcaatcct tgttcataca gcgcatgtcc gccttcgtgg
    33721 atacagctgc caatcatttc aaacaaatca ttctcactga tgcgtgtggt tacccgcaca
    33781 tcattggcag aaaacgaagt ggtgaaagga tgtgtagata tgtcctgcct tccgctgtca
    33841 aaatcgtaat gcatttgttt caacaggtct aatccaaaat gccactgagc atctttattg
    33901 tattgtttaa acaagaaact gtcatcaggt tgtggcttct gcactaattg ttgaatgtaa
    33961 ggcacaagct tctgccttac ctgattaaaa attgtttcca gaaaacttgt cttcagccct
    34021 gtttcataga ggttgagatg tgcatcatag ggatgttgct cataacccaa taactccgat
    34081 tcctgtcttt taaactttat cagttcctga aggtgcggtt caaacaactt aaaattattt
    34141 tcttgccttg cctgttgcca tgcattaaag ctttttgaaa tacattgatt gagtgcaact
    34201 acaaattctg tggtatattt tttctgttgt ctgaaaacac gatacgactc cagcacattg
    34261 gcacattgca ccgggctgag tttggtagta tcatcatgca gtttttctat aatctcttca
    34321 tagcgtttat cagttatcaa ctcatgtgca tagcctgcca gagttgacag ttgctgagcc
    34381 cgataatgtg cgcctttggg aggcatataa acttcctgat cccattgcag tacagcactg
    34441 ctgtaattga tgtctgcaat cttctgaagg tgggttacat attgttgata tagttcactc
    34501 attattattt tttaaatgca tgaattatta aaaaaagcca tcctgtcata aaacatagtc
    34561 cgccaacagg agtaacagga ccaagccatg cccaggtgtt gccaccaatt gatgacaatg
    34621 ccatgccata aatcgagcca ctgaaaagta tgatacctgt cataaaaaaa gttgcagcac
    34681 ggttcagcat tttgttgtcg tttgcagcaa gtgcaacaaa caataaggca agcgtatgat
    34741 aaaactgata acgcacaccg gtttcaaatg ctgacaacat ctgtggtgat aacaattctt
    34801 ttagcttatg tgccccaaag gcacctaatg ccactgcgca aaggccggat atggtggcaa
    34861 atattaaaat cttcctgttc attattcttt atttcctttc tcacctacaa aaacatcttc
    34921 ttcgtcttta aacaaaacat taaaagtggt gaggctgcca taaatacgtg taaggtactg
    34981 ctgcaggtta attttatctt cttctgttaa tttatcatgt gcattaatgc gttgttccat
    35041 cacacgcagg cggtcgcgga tcatgatgat tttattaaag aaaacatcca taggaatttc
    35101 tttgggcttt tgtgacgggt tgttgggatg caacacaacc ttacctccta tccagcgacc
    35161 atgaagcggt actgtttcct gcacgtctga atatttttta atgatattgg taaacacccg
    35221 ttccatgtcg gcaaaactca cagggtcgcc cgcaggtggt tgagcatcta tgacttcaag
    35281 attttgataa gtcttggcaa cttcaatctt accgcgctct gtaaaaacag taatgtaggt
    35341 tcgtggcttt acatttatca caacaccaac gccaaattcg ggatgtctga tgcgtgatcc
    35401 tattcctaat tcttccatat aaaagtttta taaatattat ttaatgccta tgctgtctaa
    35461 ctcttgaatg gtaacagagg gtggcatgat attgtttttt agtttaaatg atacaagtgc
    35521 tgctgttttt gaggcatctt cgtctgtgtc aaatccacgg ttacccataa ctgccggtat
    35581 gttgggctga tgcaccataa tttttccatc aactaaaata ttgtagccaa agccacctaa
    35641 ggtactgtcg tt
//
LOCUS       DAOQNP010000023        34651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_402_length_34651_cov_8.399757, whole genome shotgun sequence.
ACCESSION   DAOQNP010000023 DAOQNP010000000
VERSION     DAOQNP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 34651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_34651_cov_8.399757"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>684)
                     /locus_tag="PKY09_04955"
     CDS             complement(<1..>684)
                     /locus_tag="PKY09_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12239.1"
                     /translation="NPPLITDNALIPGSSPNRPVIKEGTFATAKKLRIQGTDTVTVNV
                     YKTGRLTVADSLIINDPASTLKVNSQFTDTARISVGNVYPTIPPYYPLNAAALKQRCQ
                     IVYTGPELSSYGMITGDEIDSIFLLVSNFTAPNNPYTNFSIRWYYTTAIFSYGGTAAN
                     NALAPFAVGTTPNLVYTTPSLGIPSAGQYKFGLSTPIPVNLAYSLVIDICYDHAIGGG
                     QQTWYTNTTG"
     assembly_gap    685..708
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<709..2520)
                     /locus_tag="PKY09_04960"
     CDS             complement(<709..2520)
                     /locus_tag="PKY09_04960"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNB12240.1"
                     /translation="MQKFYSFIVCFALAFLCTVSNSSGQLVINESFNGTTFPPLGWST
                     NYSGTSSDAENYCERVTVSSNPAGTPPGTHSGAGMARYRCGYMTQAGEIAYIASRALD
                     FTNMPVGGTTATFWMYRDAVNTGFLDSVSVYLNDSNLIAFDAGNANVGDVQRLAIQQV
                     APFGFGNTVPRWWNANPTCGAAGWYQYQVTIPNNAFTVASQRYLIFVFTNRHTLANGG
                     NIYIDDISINTYPKAQSFVSTRMSFQGTSSVPPGTTDNLIYGVEVVIDGGDDVTANNY
                     YTIDSLHFSYIGSTNACGDAQNAKLWWTGGTDVFNGIANAIQVGATVPTLCATNYSFI
                     PIATFRLNNGKNYFWVTYDIKPAGVSTPGNCVDGEYNFIRLRKGASAVNYTPNPATLG
                     GCRPIDVAYCGGATPSYSTGTSWLGGSYTNNDYVAQVSLNGESGYPVINNALNCCGPN
                     IAPWWGGPAPFSAHPPDYEKFAAVPGKTTVLMANGMTSDTINLKVGTYCCLNYIAAFI
                     DFNHDGDFYDAGEKIAQSPAMSANGTYSVAFTVPVSAYIGTTTLRVREVYGSTQGNNI
                     DPCASAFFGETEDYSITIIPSCNSWPGLGYGKLWLGGV"
     gene            2828..4096
                     /gene="serS"
                     /locus_tag="PKY09_04965"
     CDS             2828..4096
                     /gene="serS"
                     /locus_tag="PKY09_04965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNB12241.1"
                     /translation="MLELSKIRENPQEIIKKLAIKNFNASENVALILQLDEKRRQLQK
                     ENDTCLAAQNKMAAEIGTAMKTGKKEEAEQLKAKNAALKETTKKHQSELEIIENLLQD
                     ELVKLPNLPSEKVSPGKSAADNDIIHTVTALPKLTTKIPHWELCAKYNLIDFETGVKL
                     TGAGFPVYRGKGARLQRALINFFLDKAVAAGYLEIQPPILVNQDSGFGTGQLPDKEGQ
                     MYFVTEDQFYLIPTAEVPVTNIYRNTILKESDFPQKLTAYTPCFRREAGSYGKDVRGL
                     NRLHQFDKVEIVRIEHPENSYKVLEEMKDYVCSLLEQLELPYRVLKLCGGDMSFASAL
                     TYDLEVFSAAQEKWLEVSSVSNFESFQSNRLKLRFKGNDGKTQLAHTLNGSALALPRI
                     VAALLENNYNGHYIHIPKAIQHYTGFDRID"
     gene            4099..5937
                     /locus_tag="PKY09_04970"
     CDS             4099..5937
                     /locus_tag="PKY09_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12242.1"
                     /translation="MFITRQFQQLKKTVFIIAPLILLSIISSAQGLNNLDLARQYEST
                     GEFDKAAVYYEKQYNIDPFGTYEPYLKCLKQIKDYKEAEKLIKKQAKRGAGAGKYNVD
                     LGLLYELEGDNEKAKKQFETAIKNLHPDAGDIYAVANSFIMSGKPDLALETFLKGRSM
                     VPDNNFGFDIAELYGRKGETQKMIDEYLDIMTENTMFQANVQSVLQYKLSQDSDSNLA
                     NLLRQSLLRKIQREPSQLIYSEFLYWLFMQEKDFESALIQAKALDKKTGDTGNRIYSL
                     GEICISNQAWTTAEKCFDYIVAKGSSHPLYIRAKIAMLSAAFEKTTESHYTQAELLTL
                     EKQFTETLQELGRNSITAPLMVKQAQLEAFYLDKVAEGRKILEEVIAMPALSPNYIAE
                     VKLALGDIMILDGDLWEASLTYSQVDKDFKHDAIGREAKFRNARLSYYLGEFEWAAAQ
                     LNVLKQATSQLISNDALDLALLISDNIGDDSITEPLQIYSRAELHAFQHKNDLALQTL
                     DSILVLYPGRKITANVWFKQAGIYFTKGDFEKAIALYSDIVKNYADGVLADDALFRIG
                     SIYENNLKDTEKAKSTYEQFIDKYPSSVFIAEARRHYRLLRGDKIN"
     gene            6030..8090
                     /locus_tag="PKY09_04975"
     CDS             6030..8090
                     /locus_tag="PKY09_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNB12243.1"
                     /translation="MQQSLRIILTAIILFFSTGSKAQIFEGAKWSFRVEQTDSCEATL
                     ILKAVTVDKFHIYSQTINGADGPIPTSFTFNHSPEYTTVGKVKEGHAEEVFDQQFQMK
                     LKFFHGETEFKQKIKINTRKKFSITGFVTFMACDDEKCLPPTDIDFKFDLNDGKKKSC
                     ATTELAVPVNNATPAVTDTAQAVMPPERIDSATAAAQNDSVFTEVAQLSELEPGCGDG
                     TTTTATDRSMLGIFIAGFLGGFLALLTPCVFPMIPMTVSFFTKRSGSRQKGLMNALIY
                     SISIIVIYVGLGFLVTVTLGSDALNDMASNVFFNMTFFIVFLIFAFSFFGAFEIVLPS
                     WLVNKADSASDRGGLIGIFFMAFTLTLVSFSCTGPIIGTLLVEAAHNGSYLGPIIGMT
                     GFALALALPFGLFSAFPGWLNSMPKSGGWLNTVKVTLGFIELALAFKFLSNVDLAYHW
                     GFLKREIFIAIWVIIFGMLGFYLLGKLKFSHDSDVKHISVPRLMFAIISLTFTLYLIP
                     GIWGAPLKMISGFPPPEFYKEWVTEKSSNHCPHNLNCYHDYDAGMAYAKTVGKPVMVD
                     FTGWSCVNCRKMEDNVWSDPEVLKRLSDKYVLISLYVDDKEQLPENKQYVSPTTGKKI
                     KTIGNKWSDLQTNVYKTNSQPYYVLLDHQGKILAEPRGYTPDIKTYTKYLDEGLCRFE
                     KRKK"
     gene            complement(8140..8523)
                     /locus_tag="PKY09_04980"
     CDS             complement(8140..8523)
                     /locus_tag="PKY09_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNB12244.1"
                     /translation="MKKSYIIAMVLIAVAFAAIISTVSDSGTYANFNEASSHEGKVFH
                     VVGKLNRDKPYEYHPEKNANLFTFYLYDNNGNERKVLLNKAKPQDFDKSEQIVIIGKM
                     KGEEFIASDILLKCPSKYADEQQKS"
     gene            complement(8532..8759)
                     /locus_tag="PKY09_04985"
     CDS             complement(8532..8759)
                     /locus_tag="PKY09_04985"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNB12245.1"
                     /translation="MEFSRKLKILFSVLMLVATNVFAQTESALDETFRSNGKIYVVVA
                     VLAVVLAVLFIYLISIDRKLKRTEDKLKNKY"
     gene            complement(8738..9394)
                     /gene="ccsA"
                     /locus_tag="PKY09_04990"
     CDS             complement(8738..9394)
                     /gene="ccsA"
                     /locus_tag="PKY09_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNB12246.1"
                     /translation="MPNVWKIACVVLLYFTLFAGFLMPVPELPILNESIRNLYFHVTM
                     WFAMIIHMIVTIIYSIRYLAGGKIQYDLKAEQYAITGMVFGIAGLITGMIWARFTWGA
                     FWVNDTKLNGAAAAMLMYAAYFILRQSTEDDTKRAKLSAVYLVFAFPLMMAFIYILPR
                     MTDSLHPGNGGNPAFGQYDLDNQMRLVFYPAILGWTLLGVWITSIRIRIKKLWNSHEN
                     "
     gene            complement(9432..10088)
                     /locus_tag="PKY09_04995"
     CDS             complement(9432..10088)
                     /locus_tag="PKY09_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNB12247.1"
                     /translation="MFNQIRLIIFKEWKLEWRNRAAMAGLMVFILSGVYISYLSFKKI
                     VDVSAWNALFWLLILFMAVNALAKSFMAESRGLQLFYYSFLNPYAVILGKIIYNGLML
                     FLFGIITFFIYGILLGNPVQDMGMFILTVLLGCFGISSVLTMISAIASRAGHQSSLMA
                     ILGFPVLLPLLIVLIHLSKNAIDGIDFSVNLKYLTMLTALNLLVPSLAYILFPYLWRE
                     "
     gene            10303..12471
                     /locus_tag="PKY09_05000"
     CDS             10303..12471
                     /locus_tag="PKY09_05000"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNB12248.1"
                     /translation="MKKVLLTLLAATTIISANAQRKAGINIKEQPTITQRQCGTGELP
                     LEYETWLAAKMKEPNANKMQVVYTIPVVVHVIHNGTNVGVNQNISDAQINSQIAVLNE
                     DYRRLNPDTTSTPSVFGAVAADCEINFCLAQRDPSGNPTNGIDRINRNTQGWTAPPYT
                     KTYIDGTIKPASIWDPTQYLNIWVVPDYSSSGSQLLGHATFPLNSTLTGLTGNYGTAT
                     NDGVVVWYKAFGRVGTLDALYNKGRTATHEIGHWLGLRHIWGDANCGNDYVSDTPTQQ
                     TANYGGQNPPYPCPSFPLVTCANGPNGDMWMNYMDYCYDKCLNMFTTGQKSRMQIAMA
                     NGTYRAPLATSQGCNAPSAGPTAQFTANTTTIAPGGSVNFTDQSTNSPTSWSWSFPGG
                     TPSSSTAQNPTNIVYANAGTYNVSLTATNANGSNTLTKNNYIVVTAGGTSSCDTISNF
                     ALATDTVTIYLAGTAPGSGYLSGHNSYLDQSKADKYANATPNTTVDGAIIFFGVGTSS
                     GTGQTASAKVWNASGTGGMPGTAIGSVNITYDQIAADATGGLPTIVDFNPNVSIAVGN
                     YYVGMNFAYNTGDTLAIITNRDGNTVPGTGYEQFDTGTWYAYSNTTSSWGINVAHAIF
                     PIVCTSTGIREVMTPGNNLMVFPNPSNGEFTLVVPQSDLKENVVVRVIDVKGAVVLNK
                     ELISSGNGTYRLKLDAPAKGIYMLEVQTVNGLKKQRISVN"
     gene            12616..14538
                     /locus_tag="PKY09_05005"
     CDS             12616..14538
                     /locus_tag="PKY09_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB12249.1"
                     /translation="MFKRILIILFAFATATAFAQKQTYTSTSSRAVKNLEQALKYYDS
                     RQTAKAISETENAIKADPAFIEAYMLLGDLQADNNNIPAAIEAYKKAIAINPRFFPNN
                     FYALGRYELESGRYDDALTHFNEFVKMETAKADKKEEAKNLIASCEFAKKAIQNPVPF
                     SPVNAGAGINSDMDEYFPSITVDSKRMLFTRKIKSDLYEWQEDFYISTLQQDSTWGKA
                     KNVGAPVNTALNEGAPSYSADGQLIFFTACDRPESKGSCDIYYSRKFGDAWGKPINLG
                     SPVNTGAWESQPSFSSDGKTLYFVRGSNSRTNDQDIYMSEVSEKGWSTPVKLNNNINT
                     AGKEEAVFIHPDNQTLYFVSDGWPGMGGSDIFMSKRQADGQWGKPINLGYPINTFANE
                     AGLIVSPTGDKAYFSSNREGGLGGLDIYSFDLYDKIKPDPVSYAIGYVYDDETKKPTD
                     AKFEIIDIESGKTVVESYSDKNTGEFMVTLPYGKNYMLNVSKRKYLFYSDHFEMDKLA
                     DIKNAKQLIVRLKPVKDGSTMSLNNIFFDTNSAALKPESKTELNKLVSFIKSNAIVKL
                     EIGGHTDNVGDDKANMILSDKRAKSVCDYLITSGIEASRLVSKGYGETKPVAENTTEE
                     GKAKNRRTEITILSVH"
     gene            14637..16196
                     /gene="dnaB"
                     /locus_tag="PKY09_05010"
     CDS             14637..16196
                     /gene="dnaB"
                     /locus_tag="PKY09_05010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNB12250.1"
                     /translation="MEENVEAGRSAKKKVAPKSLTNQQIALGLGKIPPQAVDMEEAVL
                     GALMLEKNALTAVIDVLHTEAFYKEAHQKIFKVIHELFHKAEPIDILTVTHALKKAGD
                     LELVGGAFYITQLTNRIASSANIEYHARIILQKFIQRELIRISSETIKSAYEDTSDVL
                     KMLDEAEKNLFAIAEGNIRRNYENMNSLISKAIKEIEIASKQTQGITGVESGFSELDR
                     ITAGWQKSDLIIVAARPGMGKTAFTLSLAANAAIQFNRPVAFFSLEMSSVQLVNRLIS
                     AQAEISSESIRKGQLEPYEWQQLNSKIVPLSDAPIFIDDTPALSVFELRAKCRRLVAE
                     KKVQLIIIDYMQLMVAGTDNRNGNREQEISTISRSLKAIAKELEVPIICLSQLSREVE
                     KRGTASKRPQLSDLRESGAIEQDADMVMFIYRPEYYGMDVDENGDSTSNTAEIIIAKN
                     RNGSLNNVKLTFIGQFTKFKDLEVKRQEDPSYHYTDESSKVRMPNPNVEAENRYTRGS
                     RMNDMPDEDLV"
     gene            complement(16203..17105)
                     /gene="rfbD"
                     /locus_tag="PKY09_05015"
     CDS             complement(16203..17105)
                     /gene="rfbD"
                     /locus_tag="PKY09_05015"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNB12251.1"
                     /translation="MLRILLTGSNGLLGQKIVYAALKRNDIQLLATSKGENRLHAKAG
                     YQYASMDITNADEVTAIVENFKPHCIINTAAMTNVDACEKDQENCWKMNVEAVKTMTE
                     LCQRHNIHFIHLSTDFVFDGEKGNYSEEDVPNPLSHYARSKYESEKVVQQAKIDAAVL
                     RTIIIYGVVDDKQRSNVVIWTINSLRQQKDINVISDQIRSPTLAEDLADACVQAALKR
                     ATGIYHVSGSETISILDMVNRTADFFQLDKKYIHPITTAQLNQPAKRPLLTGFNIDKA
                     KRELNFRPHTLQQGLEIVKKQLEN"
     gene            complement(17118..19421)
                     /locus_tag="PKY09_05020"
     CDS             complement(17118..19421)
                     /locus_tag="PKY09_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12252.1"
                     /translation="MTIKRIFLAAFCMLAVSLANAQTATIKGFVYAAENGEPVPFASV
                     FLKGTTFASFTDINGYYSMVKIPPGKYRVMATTLGYDTAFADVELVADEMENRKLVMK
                     KKNVELKQVEVSAERQTKKTEVKIAVESVTPQEIKSLPSLGAEGDLVQYMQVVPGVVS
                     SGDQGGQLYIRGGTPVQNKVLLDGMVIYNPFHSIGLFSVFDTDILKNTDVYTAGFGAQ
                     YGGRISSVMDITTRDGNKKRIGGKVSTGTFLSKVLLEGPIKKAKDENDGTASFILSVK
                     NSYLDQTSKSIYKYASDDGLPYSFNDIYGKVVINSASGSKVSFFGFNFLDKVNYSKVA
                     KYDWSSSGGGMNFVLVPGASKVLLRGNLAFSSYKIKLTEAEEKPRSSLVNGFNMGFNW
                     LYFLNNDEINYGIEVQGFKTDFEYFNYAKRQINQTENTTEIGIFFRYKKIINKLIIDP
                     SVRLQYYASISEFSPEPRLGLKYNLSDNIRLKAAGGLYAQNLIAANSDRDVVNFFYGF
                     LSAPDDLPSEFDGKSVKKKLQLARHAVVGVEYDINKYFDINIEGYIKDFNQLTNINRD
                     KVYDDTANNFDKPDSVKKSFIIEKGFTQGVDVVLKYEYRRMYAWLVYSLGFSTRNTGN
                     YEYAPHFDRRHNINIIGSYKLGKKLDWEVDARWNFGSGFPVTLNQGFYPYLNFQDGLA
                     TDYTQNNGEMGVLYGPLNESRLPTYHRLDISIKKIFYLVRNSELEVVASVVNVYNRKN
                     VFYVDRIRHNVVNQLPIMPSLGASLTF"
     gene            complement(19427..19831)
                     /locus_tag="PKY09_05025"
     CDS             complement(19427..19831)
                     /locus_tag="PKY09_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNB12253.1"
                     /translation="MKYWLVKSEPFKYSWEQFVADGKTFWDGVRNNAARLHLLAMKKG
                     DQVLFYHSNEGLAVMGIAKVEKTAYSDPTADDARWVGVELTPFKALKKPVTLAQVKED
                     ARLKDIALVRIGRLSVMPLKKEEFNVILELSK"
     gene            complement(19882..20469)
                     /gene="purN"
                     /locus_tag="PKY09_05030"
     CDS             complement(19882..20469)
                     /gene="purN"
                     /locus_tag="PKY09_05030"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNB12254.1"
                     /translation="MNKKRIAILASGSGTNAENIAAYFKNNEYAEVAVILTNVATAGV
                     IARAQKLNIPYFFMDKKTISDGRLMLDLIKQHNIDLVVLAGYLKLMPAEVVSAFQHRM
                     INIHPALLPLFGGKGMYGHNVHEAVLQAGVKETGITIHYVNSRFDEGEIIFQKSMPVL
                     PDDTAESIASRIHRLEYEHYPPVIEQLAKGLFKDN"
     gene            20608..21429
                     /locus_tag="PKY09_05035"
     CDS             20608..21429
                     /locus_tag="PKY09_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNB12255.1"
                     /translation="MYFCTNIKLLRKRKKRTQEVASLALGFSRSTLNSYENGAIANPT
                     VEALINFSNYFKISIDTLLRVDMSKLSDLQLRELELGYDSYVRGTKLRVLATTVDSRN
                     RENIELVPVKAKAGYTAGYNDPEFIRNLPTFQLPFLSHDRKYRTFQISGDSMLPIPDK
                     SFVTAEFVENWMEIKDGNAYIILTHDDGIVFKVVYNHISSNKKLLLKSLNPAYKPYEV
                     KINEVKEVWRFVNYISNEMPDTGLQNPKLSNTVARIQNEMNKIKDLLGVQDVSKE"
     gene            complement(21600..23309)
                     /locus_tag="PKY09_05040"
     CDS             complement(21600..23309)
                     /locus_tag="PKY09_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12256.1"
                     /translation="MTVLRIKLLLFSGIIILLSLTLQKSWAQQTQWAVQLGAQDVNAM
                     CGDSHQNIYATGSFIGNVDFDPGPGYVYLPFNPGGNNTYITKMDSLGNLIWAKSIVGS
                     DVISWGVATDSTQSVYIAGLFYSSADFNPDTLINYTLTATNASSGNPDIFVLKLDSLG
                     NFIWAFKIGTGGFESAGNLVVDQSGNIYITGRYDNTCDFDPGSGVANLSTSTPESVYI
                     AKYDSAGQYVWVQAAQLSYYGEYERMAAGYDHNGNIYIANYGEIAKVDTAGNFVFKKS
                     FNNNPVSTQGYCNVTSLSCDYLGNVFVCGYFNDTIDFDTDSTTAFYVNAVGAYNTFVC
                     HLNSSVELVWVRQFTQNSTNLSVAANHAHAILVKDTLVYVGGVFTDTMDVNMPAGVNM
                     LYEVAATPQDDYYDAYVVALTNTGNYAWGESFGGNLLDRSTTLCASDDAVYVAGQFSD
                     TADFNNSPLVQTLMFNGVGNFNVFVLKFNAPAITTIKEIKSEELQYFVYPVPVKKVLY
                     FSENILKNFYTFSVKDIAGREIIHSKLMSGKLNVDALSNGVYIIEFFKSNNENFKAKF
                     IKE"
     gene            complement(23498..23923)
                     /locus_tag="PKY09_05045"
                     /pseudo
     CDS             complement(23498..23923)
                     /locus_tag="PKY09_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(23984..24475)
                     /locus_tag="PKY09_05050"
     CDS             complement(23984..24475)
                     /locus_tag="PKY09_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNB12257.1"
                     /translation="MPSFDIVNEIDIQKLDNAINVAKKEIVNRYDFHGSKTEIDLDKK
                     EMQLKIITEDQMRMDAIRDIIISRSMKQGVDPKCFDFGKEQYASGNMVRKDVKIKKGI
                     DKETAKTIVKFIKETGMKVQASIMDEQVRVTGKKIDDLQQVIAEVKNKPVELPLQYVN
                     FRS"
     gene            24592..26853
                     /locus_tag="PKY09_05055"
     CDS             24592..26853
                     /locus_tag="PKY09_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12258.1"
                     /translation="MKINIISHVLRHAMLCLTVFITVNSVFAQNYSKYTSPKVFVNNQ
                     ALKNPWSGGINSPLYSTIDLNNDGIKDLFMFDKQFGAAYPGSYKIRTFINQGTAGQVD
                     YIYTPFYERFFPQNLHDWVILVDYDCDGNEDIFTYSYSGGMEVYHNDITPGNNLSFSL
                     TYPLIHSLFLGSAYANLYVSNVNLPVLHDVNGDGDLDVLTFTLLGGTIEYHENYAKEL
                     YNRCDTLVYRQVRQCYAQVGLSAVSNTAILDFNNCFFLAPPPLPDSMIKTPRHNGSCM
                     IGYDQDGDGDLDLINGDILGNNLLYLENSGIPGVSDSIHLQDTLFPSYNVPVIYKTFP
                     APFYFDADNDGAKDLIVSSCTEYLTEDYQNSWFYKNVGSNTNNQFQFIKKRFLTDEMI
                     DVGSGAYVRFFDVDNDGKKDLIIGNYGYFTPDSASGHYESALSYYHNITTGPLPEFEF
                     VTDDFNNLRSLGRTGLNATFGDLNGDGHDDMILGDKDGILHYYLNNGTTPATFTLAPN
                     GFNFQNIDVGTNSFPFLADINNDGLLDLIIGERYGYIYYYQNTGSATNPVFTYVTNKL
                     GNVFVGNAQSFYGFSYPHFYKENGVSKLLVSNENGYIQKYDNIDGNLGGTFNQVSTGM
                     FSVFEPYQTSFDMADLNGDGMDEVITGCYAGGLSIYSKNLPVGIAENIHPEKEITVYP
                     NPAKNYLVFEMNKNNAHNGKYQLSDISGKVVLEGSFQSYINNISLQGLTKGIYILQVT
                     ESNHFSSHKVVKF"
     gene            26855..29116
                     /locus_tag="PKY09_05060"
     CDS             26855..29116
                     /locus_tag="PKY09_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12259.1"
                     /translation="MKFFSFFLFSLLIVHQTSTSQTYNKYTSPVVIKNGQPLRNAWAG
                     GFNSAMFQAIDMNNDGKKDLFAFEKQSESTYPGVFKIRTFINHGSAGNTDYRYDPFYE
                     KLFPQNMTNFCLLVDYDGDGREDIFTYNRSVSGFDVYHNDSNVTGVLTFSLEYQGLQT
                     CYLNAGGNCFTTSLYVAPVNQPVLFDINNDGDLDVLTFQISANFIEYHENMAKELFNR
                     TDTLVLSEVNSKWGHMSLSSNANSAILGMPRIPLTNGNADVLSNRHSGTCMIAFDDDG
                     DGDADLLNGDLLGDNLLYLHNGATNNGTDSIDWQDTSFPTNGGPVNYVTFPSAYLLDA
                     DNDGITDMIVSSCTESQAENLNNNWYYHNAGSNTAPDFQYVKNKFLTDEMIDVGTASM
                     PVFFDVDADGKTDIVIGNKGYFITQSGPAHYEAALSWYKNTSTGPCPEFTLVSSNFGS
                     FQQYNYKNIYPAFGDLDGDGDADLLLGNEDGTFHYYTNTAGVGNIPQYTLTSGGFNFQ
                     NLDVGSNSTPQLYDINNDGLLDLISGELFGKISYFQNTGTVTAPIFSLITPTFGNINV
                     TNTAQTFYGYSIPHLFSDNGSLKLLVGSESGDLFLYDSISTTAGSTFHLISNNAYLIH
                     EPPRAAPAMADLNNDGSPEIIVGNQAGGLSFYSNSLPGCPLSINNILSDNQVNIYPNP
                     ANSYLIIDTDDADGMKKLITLTDLSGRIIHSQNTFSRKTLLNTEGLANGIYLIILTAQ
                     SNNITKKIIIRHD"
     gene            29109..30596
                     /locus_tag="PKY09_05065"
     CDS             29109..30596
                     /locus_tag="PKY09_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12260.1"
                     /translation="MTKRYFLFTTLWAFTLFLTSCTWDKMDEVAPPTDSCSQYPPEIS
                     AIISNKCATAGCHNTLSYSASSGLNLTNWASMIKGNDAGAVVIPGNADYSTLFSFCNI
                     YSDLGFTGNEPKMPYNETALSHSEVSTIKNWIINGAKNACGQDIFPQDDNRKKVYISN
                     QACDQVAVLDEASGLLMKWFDVGNIAGFTEAPHMIRLTPDEKYFLIAFFSSGIIQRYR
                     TSDDSFVDQVDFSNGMPGQWGTLVISPDSKRAYSVDWSASGRIAVLNLENFPMTSFTS
                     LPIINLAYPHGMAFNSTGDKLYITNQNGSTYYVVDTIGSPSDFPQWLSNIQTFSYGGG
                     LNGHEIIFSPDYSKYFISCDATNEVRVFDANTHALLANIPVGTLPQEFSLSLKKPYLF
                     VTCTEDIINGKRGSVAVIDYNNLTLIKKIHTGTQPHGVAVNDDKGVVYVANRNVDANG
                     EAPHHSGACAGRNGNLTQIDMNTLELVPGYKCELGVNPYSVAYKK"
     gene            complement(30597..31823)
                     /locus_tag="PKY09_05070"
     CDS             complement(30597..31823)
                     /locus_tag="PKY09_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12261.1"
                     /translation="MAYRITKPDRSVEGVVAVPVSKSEAGRIAIINALRGVAQNNDCL
                     ATDTVKLQELLFSEEHTLDAQDGGTTARFIMAYCVVRNRAAVITGSPRLRQRPMSCSV
                     DLLRVLGAEISYLEEEGFLPVSVQAAKLKVPDVVQTSAEKTSQHISALMMIAPLFGKD
                     FTIELTGILSSLPYIELTADLMQQVGVNVQMTGNRIQISGSYNNNILSAGGDWSAASY
                     FFETAALADMADITIENLNSLSKQGDKVIAQMVKSFGVDTIVEKDKIILKKQKNIIPD
                     YFTHSFLHYPDIALSMAAVCGGCDCKADLLHLHNLIIKESDRVSAFQREAYKLNIKTD
                     FCGGSKLKILEESNLKPSKRILKTYNDHRLIMAFAPLALKTGELFLDDISNVSKSFPD
                     YFEQMKSLGFSIEEVI"
     gene            complement(31813..32883)
                     /gene="aroB"
                     /locus_tag="PKY09_05075"
     CDS             complement(31813..32883)
                     /gene="aroB"
                     /locus_tag="PKY09_05075"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNB12262.1"
                     /translation="MIGFADSSYILSGIRKQELIDFLHDCNNQKRNIFVLCDTNTIEY
                     CLPLFQTLFPQTKSASVYCIQSGEAYKTIETATAFWKFLLQQQADKHTVIVNLGGGVI
                     TDLGGFAASLFKRGVDFIHVPTTLLAMVDAAIGGKNGVNIDGFKNQSGSIVIPRYVYI
                     DTEFLKSLPHVEKVNGYAEMIKHALVADKLFYKSLSEQRNFEALQIIEKAIAIKQSIV
                     ASDPYEINERKLLNFGHTIAHALESMYHLQRQNISHGKAVAVGIIVESYLSCKMGFIS
                     EAALNEITGFVQSLFKFDDKDFNVEKLLPFMMADKKNKQGQLRFTLLESIGKGVVDIP
                     VPIAMIEDAFHWFSKQNPGYGI"
     gene            complement(32888..33994)
                     /locus_tag="PKY09_05080"
     CDS             complement(32888..33994)
                     /locus_tag="PKY09_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HNB12263.1"
                     /translation="MFNNFHHSLKSLTEPLWIAGPCSAEDEQQLTELAIRLKIGGAHI
                     LRAGAWKPRTRPGAFEGFGAPALKWIRAAGDASGLPVITEVASAKHVEQALASGIDML
                     WIGARTTVNPFLIQEIADELKGVDIPVFVKNPVNPDVKLWMGAIERFMQNGISRIASV
                     HRGFSSFENSTYRNKPNWELAIELRRLLPNVPIICDPSHISGDAKMVPTLTQIAFDLQ
                     YDGVMVEVHLNPAKALSDAMQQITPEDFSHLRKQTIVRQPTAKDPVELSKLHDLRDLI
                     DELDNEIIEKIAGRMEVVRKIGQYKDQNNMAILQLDRFKEILRTRTETGLHNQLTPDF
                     ILRLYSLIHEESILQQDKQMTKPELRASSKTEKH"
     gene            34140..34221
                     /locus_tag="PKY09_05085"
     tRNA            34140..34221
                     /locus_tag="PKY09_05085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34174..34176,aa:Leu,seq:gag)
     gene            34253..34336
                     /locus_tag="PKY09_05090"
     tRNA            34253..34336
                     /locus_tag="PKY09_05090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34287..34289,aa:Leu,seq:cag)
BASE COUNT        11001 a         6702 c         6548 g        10376 t
ORIGIN      
        1 cctgttgtgt tagtgtacca ggtttgctga ccaccaccaa tggcatgatc ataacaaata
       61 tcaataacca atgaataagc aaggttaaca ggtatgggtg ttgataatcc aaacttatac
      121 tgacctgctg aagggatacc aagtgaaggg gtggtgtaaa ccaaattagg tgttgtacca
      181 actgcaaacg gggccaaagc attgtttgcg gcagtcccac cataagagaa aattgcagtg
      241 gtataatacc atcggatact gaaattggta taggggttgt taggtgctgt aaaatttgat
      301 accaacagaa agatagaatc aatttcatca ccggtaatca ttccataaga gctcaattcg
      361 ggacctgtat aaactatctg gcagcgttgt tttaatgcag cagcatttaa aggataataa
      421 ggcggaatgg taggatatac gttacccaca gaaatacgtg ctgtgtctgt aaattgcgaa
      481 ttaactttta aagttgatgc cgggtcattg ataattaatg aatctgcaac agtaagacgg
      541 ccggttttat aaacattaac agttactgta tccgttccct gaattcttag tttttttgct
      601 gttgcaaagg tgccttcttt aattaccgga cggttcgggc tgctaccggg aataagtgca
      661 ttatcagtta ttaatggtgg atttnnnnnn nnnnnnnnnn nnnnnnnnaa ccccgccaag
      721 ccacaattta ccatagccca aacccggcca tgaattacat gaaggaatta tagtaattga
      781 ataatcttct gtttcaccaa agaaagcaga ggcgcagggg tctatattat tgccttgcgt
      841 tgaaccatac acttctctca ccctgagggt tgtggtaccg atataggcac ttacaggcac
      901 cgtgaaagct actgagtagg tgccattggc agacattgca ggcgactgcg caattttttc
      961 gccagcgtca taaaagtctc catcgtgatt aaagtcaata aatgctgcaa tatagtttaa
     1021 acaacaatag gtacctactt ttaaattaat ggtatctgaa gtcatgccat tggccatcaa
     1081 cacggtagtt ttaccgggca cagcagcaaa tttttcataa tcgggtgggt gggcactaaa
     1141 aggtgcaggg ccaccccacc aaggggcaat attcgggccg cagcagttca atgcattatt
     1201 aatgacagga taccctgact caccgtttaa gcttacctgt gccacatagt cattgttggt
     1261 ataggagcct ccaagccacg atgtacctgt ggaataagat ggtgttgcgc caccacaata
     1321 agctacgtct atgggtctgc agccgcctag tgttgccgga tttggcgtat agtttacagc
     1381 actggcacct tttctgagtc ggataaaatt gtattcaccg tcaacacaat tgcctggagt
     1441 agaaacgcca gcgggtttaa tatcgtaggt tacccaaaaa tagtttttac cattgttgag
     1501 cctgaaggtt gctatgggaa taaatgaata gttggtagca cataatgtgg gcactgtggc
     1561 acccacctga atggcattgg caatgccgtt aaagacatct gttccacctg tccaccacag
     1621 tttggcattt tgcgcatcgc cacaggcatt ggtagagcca atgtaactga agtgcagcga
     1681 gtcaatagtg taataattat ttgcagtaac gtcatcacca ccatcaataa caacttctac
     1741 tccataaatt aaattatcag ttgtgcccgg aggtacagaa gaagtaccct gaaaagacat
     1801 tcttgtagat acaaaagact gtgcttttgg ataggtgttg atagaaatat cgtcaatata
     1861 aatattgcca ccgttggcta atgtatgcct gttggtaaaa acaaaaatca aatatcgctg
     1921 tgatgctaca gtaaatgcat tgttggggat agtaacctga tattgatacc aaccggcagc
     1981 accacaagtt ggattagcat tccaccaccg tggcactgta tttccaaaac caaatggagc
     2041 aacctgttgg atggctaaac gttgtacatc accaacattg gcgtttccgg catcaaacgc
     2101 aattaagttg gagtcattca gataaacgga aacgctatct aaaaaaccgg tatttacagc
     2161 atcacgatac atccagaaag ttgctgttgt tccacccacg ggcatattgg taaaatccaa
     2221 tgcacgtgaa gcaatatagg ctatctcacc tgcctgcgtc atatatccgc aacggtagcg
     2281 tgccatacct gcaccactgt gtgtacccgg aggtgttcct gcagggttgg atgatacggt
     2341 tacacgctcg caatagtttt ctgcatcgga acttgttcca gagtagttcg tactccatcc
     2401 caacggagga aaagtggtac cattaaaaga ctcattaatg acaagctgac ctgatgaatt
     2461 gcttacagtg cataaaaatg caagggcaaa acaaactata aatgagtaaa acttttgcat
     2521 gatttgaata ttaatttaat gattggtttt tatacaaata tttaagaaga caaatatact
     2581 catcaacaaa ttattaacca atgattgcag aagaaatttc accataaaga ctaaaattgt
     2641 tctttacgat aaaaacatgc ctaaatttaa tagatatgtt tctacaaatc gcattcctac
     2701 ttccacacaa agactaccac taaacaatta tggtttcttg tcttttctat ctgcacatca
     2761 aaagcctcaa taattttggt ttaatgttaa ttggttatac tgtacttttg cggacttaat
     2821 tctgtttatg ttagaacttt caaaaataag agaaaatcct caagaaataa tcaaaaagct
     2881 tgccataaag aactttaatg cctctgaaaa tgtagcatta attctacaat tggacgaaaa
     2941 aagacgacaa ttgcaaaaag aaaatgacac ctgtctggca gcacaaaaca agatggctgc
     3001 tgaaattggt actgccatga aaacaggaaa aaaagaagag gcagaacagc tgaaagcaaa
     3061 aaatgctgca ttaaaagaga ccactaaaaa acatcaatcg gaattagaaa taatagaaaa
     3121 tctgcttcag gacgaattgg taaaactacc caatttaccc tccgaaaaag tatctccggg
     3181 caaatctgct gccgacaacg acattattca cacggtgacg gcattgccga agttaaccac
     3241 taaaattccg cattgggaac tttgtgcaaa atacaacctg atagactttg aaacaggagt
     3301 aaaactaacc ggtgcaggct ttccggttta tcgtggcaaa ggtgccagac tgcaacgtgc
     3361 attaatcaat ttttttctcg acaaagcagt ggcagcaggc tatctggaaa tacagccacc
     3421 catattggtc aatcaggatt caggattcgg aacaggacaa ctgccagaca aagaaggaca
     3481 gatgtatttt gttacggaag atcagtttta tctgataccc acagcagaag ttcctgttac
     3541 caatatttac agaaacacca ttttaaaaga gagtgatttt ccgcagaaat taactgccta
     3601 cacaccatgt ttcagacgtg aagcaggctc ttatggcaag gatgtccgtg gtttaaaccg
     3661 cttgcatcag ttcgataaag tagaaattgt gcgtatagaa catccagaaa acagctacaa
     3721 agtgttggaa gaaatgaaag actatgtttg ttcgctttta gaacaattag aattgcctta
     3781 tcgcgtttta aaactttgtg gtggtgatat gagttttgct tcggcactta cttatgacct
     3841 ggaagtgttt tctgctgctc aggaaaaatg gttagaagtg agttcggtgt ctaattttga
     3901 aagttttcag agcaacagat taaaattacg atttaaaggt aacgatggta aaacacaatt
     3961 ggcacataca ttaaacggaa gtgcactggc attgccaaga attgtagcag cacttttgga
     4021 gaataattat aacggacatt acattcacat acccaaagcc atacaacatt acacaggatt
     4081 tgaccgcata gattaaacat gtttataaca agacaatttc agcaattaaa aaagacggta
     4141 tttatcatag cgcctttaat attgctgagt ataatttcat cagcacaggg gctcaacaat
     4201 cttgaccttg cacgtcaata tgaatcaacc ggtgagtttg acaaagcagc agtgtattat
     4261 gaaaagcaat acaacattga tccctttggc acatacgagc cttatctgaa atgtctgaaa
     4321 caaattaaag actataaaga agcagaaaag ctgattaaaa aacaggctaa acgtggtgca
     4381 ggtgcaggaa aatacaatgt tgatttaggt ctgctgtatg aattggaagg cgacaatgaa
     4441 aaagcaaaaa aacaatttga aacagccata aaaaatcttc atcctgacgc aggtgacatt
     4501 tacgcagttg ccaatagttt tattatgagc ggcaaacctg acttagcctt ggaaactttt
     4561 ctgaaaggaa gaagtatggt gcctgacaac aattttggtt ttgatattgc agaattgtat
     4621 ggccgaaaag gcgaaacaca aaaaatgatt gatgaatatt tagacatcat gactgaaaac
     4681 accatgtttc aggccaatgt tcaatcggtg ctgcaataca aactaagtca ggacagcgat
     4741 tcgaatcttg caaatttgtt gcgccaatcc ttattacgta aaattcagcg tgagccatca
     4801 caactcattt acagtgagtt tttgtattgg ctttttatgc aggaaaaaga ttttgaatcg
     4861 gcattgattc aggcaaaagc attagacaaa aaaacaggtg acaccggtaa cagaatttat
     4921 tccttaggcg aaatttgtat ttcgaatcag gcgtggacaa ctgcagaaaa atgttttgat
     4981 tacattgttg caaaaggaag tagtcatcca ctttacatca gagcaaaaat tgccatgcta
     5041 agtgcagctt ttgaaaaaac aacagaaagc cattacacac aggccgaatt gctgacatta
     5101 gaaaaacaat ttacggaaac actgcaggaa ttgggtagaa attcaatcac ggcaccgttg
     5161 atggtaaaac aagcgcagtt agaagcattt tatcttgata aagttgctga aggacgtaaa
     5221 atacttgaag aagtaattgc catgcctgcc ttatcaccaa actatattgc agaagtaaaa
     5281 ttagcgttag gcgacattat gattttagat ggcgacttgt gggaagcctc actcacctac
     5341 tcgcaggttg acaaagattt taaacacgat gccattggtc gcgaagccaa attccgcaat
     5401 gcacgtctat cctattactt aggagaattt gaatgggcag cagcacaatt gaatgtgttg
     5461 aagcaagcta cttcacaact gatttctaac gatgcacttg accttgcctt acttatttca
     5521 gacaacattg gtgacgattc cataacagag cccttgcaga tttattcgcg tgcagaatta
     5581 catgcctttc agcataaaaa cgaccttgca ttgcaaacat tagacagcat acttgtactt
     5641 tatccgggca gaaaaattac agccaatgta tggtttaaac aagcaggaat ttattttaca
     5701 aaaggagatt ttgaaaaagc cattgcactt tacagcgaca ttgtaaaaaa ctatgctgac
     5761 ggtgtacttg cagatgatgc cttgttcaga attggcagca tttatgaaaa caatctgaag
     5821 gacacagaaa aagccaaaag cacttatgag cagtttatag acaaatatcc ttcaagtgta
     5881 ttcattgctg aagcgcgcag gcattacaga ttactcagag gtgacaaaat aaactgactt
     5941 tgttacaaaa cttacattcc ataatgcttt gattttgtta ttttgtgcca catttaaaac
     6001 cggacaatcc ggtgaagtta aatttgacta tgcaacaaag tttgagaatt attttaactg
     6061 ccatcatatt atttttcagt actggcagca aggcacagat ttttgaaggt gccaagtgga
     6121 gtttcagagt agaacagaca gacagttgtg aagccacact gatattgaaa gctgtaacgg
     6181 tagataaatt tcatatctat tctcaaacca tcaatggcgc tgacgggcct atacccacaa
     6241 gttttacttt taatcattcg cccgaataca ctacagtagg taaagtaaaa gaaggacatg
     6301 cagaggaagt ttttgatcag cagtttcaga tgaagcttaa attttttcat ggtgaaacag
     6361 agtttaaaca gaaaatcaaa atcaacacca gaaaaaaatt cagcattaca ggctttgtga
     6421 cgtttatggc ctgtgatgat gaaaaatgcc ttccgccaac agatatagac tttaaatttg
     6481 atttgaatga tggcaagaaa aaatcgtgtg caacaacaga acttgcagtt cctgtaaaca
     6541 atgcaacacc ggcagttaca gacactgcac aggcagtgat gccaccggaa cgtattgatt
     6601 ctgcaacagc tgcggcacaa aacgacagtg tttttacaga agttgcacag ctatcagagc
     6661 ttgagccggg ctgtggcgat ggcacaacaa caactgcaac tgacagaagt atgctcggta
     6721 tttttattgc aggattctta ggtggctttt tagctttact tacgccctgt gtttttccga
     6781 tgatacctat gacggtaagc ttttttacga agcgcagcgg ttcgcgtcag aaaggattga
     6841 tgaatgcatt aatctactct atttctatca ttgttattta tgttggactc ggatttttgg
     6901 tgacagtaac tttaggcagt gatgcactta atgatatggc aagcaatgtc ttttttaata
     6961 tgacattttt tattgtcttc ctgatttttg cattttcatt ttttggtgcc tttgaaattg
     7021 tgctgccaag ctggttggtt aacaaagccg atagtgcttc tgaccgagga ggattgatag
     7081 gaattttctt tatggctttt accttgacct tggtttcttt ttcttgtaca ggacccatca
     7141 tcggcacctt gttggttgaa gctgcacaca atggaagcta tttagggcca attataggaa
     7201 tgacaggatt tgccttagca cttgcattgc catttggttt gttttctgca tttccggggt
     7261 ggttaaactc aatgcctaaa tcaggagggt ggctcaatac cgtaaaagtt acactcggtt
     7321 ttatagaatt ggcattggca tttaaattct tatcaaatgt tgaccttgcc tatcattggg
     7381 gatttttaaa gcgtgaaatt tttatcgcca tctgggtaat cattttcggt atgcttggtt
     7441 tttatctatt aggcaaatta aaattttctc atgacagcga tgtaaaacat atttctgtac
     7501 cccgactgat gtttgccatc atatcactga catttacatt gtatctgatt cccggaattt
     7561 ggggcgcacc attaaaaatg atcagtggtt ttcctccacc ggaattttat aaggaatggg
     7621 tgacagaaaa atcaagtaat cattgtccgc ataacttaaa ctgctaccac gattatgatg
     7681 caggtatggc ctatgccaaa actgtaggca agccggtgat ggttgatttt acaggatgga
     7741 gttgtgtaaa ctgccgtaaa atggaagaca atgtgtggag cgatccggaa gtgctgaaaa
     7801 ggctttctga taaatatgtt ctcatttcac tttatgttga tgacaaggaa caacttccgg
     7861 aaaacaaaca atatgtttct ccaactacgg gtaaaaaaat aaaaactatt ggtaataaat
     7921 ggagtgattt gcaaacgaat gtttacaaaa caaattcgca accttattat gttttattag
     7981 atcatcaggg aaaaattctt gctgagccac gtggttatac tcctgacata aaaacctaca
     8041 ccaaatatct tgatgagggg ttatgtcgtt ttgaaaagcg aaaaaaataa tcctgattgt
     8101 ttgtttaaca atcgttgcgt tttaataaaa aaataaagat taactttttt gttgttcgtc
     8161 agcgtacttg cttgggcact tcaacaaaat atctgaagca ataaactctt cgcctttcat
     8221 tttgccaatg attacaatct gttctgattt atcaaagtct tgtggcttgg ctttgttgag
     8281 caacactttt ctttcattgc cattgttatc gtacagataa aaagtgaaaa ggttggcatt
     8341 cttttcagga tggtattcat aaggcttgtc gcggttgagt ttacctacaa catgaaacac
     8401 cttaccttca tgcgatgatg cttcattgaa attggcatag gtaccggagt cgctcaccgt
     8461 gctgatgata gcagcaaatg caactgctat aagtaccatg gctatgatat aagatttttt
     8521 catgtctgtt tttaatattt attcttcaat ttatcttcag ttctttttaa ttttctgtca
     8581 atacttatta agtagataaa caaaactgct aacacaactg caagcactgc aaccaccaca
     8641 taaatctttc cattactgcg gaaggtttcg tccaatgcac tttctgtttg cgcaaacaca
     8701 tttgttgcaa ctaacattaa aacagaaaag agtatcttta gttttcgtga gaattccata
     8761 attttttaat tcttattcta attgatgtta tccaaacacc aagcaatgtc catcctaata
     8821 ttgcaggata aaaaacaagg cgcatctgat tgtcaagatc atattgtccg aaagcaggat
     8881 taccaccatt gcccggatga agtgagtcgg tcatacgtgg cagaatgtaa ataaatgcca
     8941 tcatcagcgg aaaggcaaaa actaaataca ctgcactgag tttggcacgt ttggtatcat
     9001 cttctgttga ttgtctgaga ataaaataag ctgcatacat gagcatggca gcagcggcac
     9061 cattaagttt ggtatcgtta acccaaaaag caccccaggt aaatcgtgcc caaatcatac
     9121 ctgtaattaa acctgcaatg ccaaatacca ttcctgttat ggcatactgt tcagctttta
     9181 aatcatactg aatttttccg cctgccaaat aacgaatgct gtaaataata gtcactatca
     9241 tgtggatgat catggcaaac cacattgtta catggaagta taaatttcta atggattcat
     9301 taaggatagg taactcaggc acaggcatga ggaatcctgc aaaaagtgta aaatagagca
     9361 atactacaca agcaattttc cagacattag gcatgatgaa aaaagaactt aaatgtcaac
     9421 atgaagaatt attattcgcg ccaaaggtag ggaaataaaa tgtaggctaa agaaggcaca
     9481 agcaaattca aagctgtgag cattgtcagg tattttaaat tcacagaaaa atcaattccg
     9541 tcaatagcat tttttgaaag gtgaattaaa acaatgagca aaggcaacag cacaggaaag
     9601 cctaaaattg ccataaggct tgactgatgt ccggcacgtg aagcaatagc tgaaatcata
     9661 gtcagcacag atgaaattcc gaaacaacct aataaaacgg taaggataaa catgcccatg
     9721 tcctgtacgg gattacctaa taaaattcca tagataaaaa aggtgatgat gccgaaaaga
     9781 aacaacatca acccattata aattatcttt cctaaaatta cggcataagg attcagaaat
     9841 gaataataga aaagctgaag accacgcgac tctgccatga agctttttgc aagtgcattt
     9901 acagccataa acaaaataag cagccagaat aaagcattcc aggccgaaac atctacaatt
     9961 ttcttaaagc tgagatagct gatataaaca cctgaaagaa taaaaaccat aagaccggcc
    10021 atagcagcac ggttgcgcca ttctaatttc cattctttaa aaattattaa ccttatctga
    10081 ttaaacacgg tacaaagttc agcaaaacaa gcattaaaat gaagtaaatg gtctaaaagt
    10141 ttaatttaaa gcctggatta aacaacaaaa tgatgaaatg ctttctattg aaaaaacaaa
    10201 taaaaataaa tgcaaattaa atttcagaat ctgcttcgcg aatttgattt ttttctacat
    10261 ttaccatccg aatttttatt tgaaaattat aaaatcaaat taatgaaaaa agttttacta
    10321 acattattag ccgcaacaac tattatttcg gctaatgcac aaagaaaagc tggcatcaac
    10381 atcaaagaac agccaactat tacacaacgc caatgcggaa ccggtgaact tccattggaa
    10441 tatgaaacgt ggcttgccgc gaaaatgaaa gaacctaatg caaataagat gcaagtggtt
    10501 tataccattc ctgttgttgt tcacgtaatt cataatggaa ccaatgtggg tgttaatcaa
    10561 aacatcagcg atgcacaaat caactcacag attgctgtct taaacgaaga ttaccgcaga
    10621 ctgaatccgg acactacctc tacaccttct gtatttggtg ccgttgccgc agattgtgaa
    10681 ataaatttct gtctggcaca acgcgaccca agcggtaacc caacaaacgg cattgatcgt
    10741 attaacagaa acacacaagg ttggacagca cctccatata caaaaacata cattgatggc
    10801 acaataaaac cggcatcaat ctgggatccg acacagtatt taaatatttg ggttgttcct
    10861 gattattcat catcaggaag tcaattacta ggtcatgcaa cattcccact aaatagtaca
    10921 ctaacaggtt tgacaggaaa ttacggtact gccacaaatg atggtgttgt tgtttggtat
    10981 aaagcttttg gtcgtgttgg aacattggat gcattatata ataaaggacg tacggcaacc
    11041 catgaaattg gtcattggtt aggtctgcgt catatttggg gtgatgccaa ttgtggaaat
    11101 gactatgtaa gtgacacgcc aacacaacaa actgcaaatt atggtggaca aaatcctcct
    11161 tatccatgtc cttcttttcc tctggtaacc tgtgcaaatg gtccaaatgg agatatgtgg
    11221 atgaattata tggactattg ttatgacaaa tgtcttaaca tgtttactac cggtcaaaag
    11281 tcaagaatgc aaattgcaat ggctaacgga acttatcgtg caccattagc aacttctcaa
    11341 ggttgcaatg caccttctgc cggcccaact gcacaattta ctgccaatac tacaacaatt
    11401 gctcccggtg gaagcgtaaa ctttactgat caaagcacca attcaccaac ctcttggtca
    11461 tggtcatttc caggtggaac gccatcatca tcaacagcac aaaaccctac caatatagtt
    11521 tatgcaaatg caggtactta caatgtttct ttgactgcta ccaatgctaa cggcagtaat
    11581 acgcttacaa aaaacaacta tattgtagtt acagcaggtg gaacttcttc ttgcgatact
    11641 atttcaaact ttgctttggc aaccgatact gttacaatct atttggcagg tactgcacct
    11701 ggaagtggat atctttcagg tcacaattca tatttagatc aatccaaagc tgataaatat
    11761 gcaaacgcaa caccaaacac aacggtagat ggtgcaatca tattttttgg ggttggtacc
    11821 tcctcaggta caggtcaaac tgcaagtgcc aaagtttgga atgcaagcgg tactggtggc
    11881 atgcccggaa ctgcaattgg cagtgtaaat atcacttatg atcaaattgc agctgatgca
    11941 acaggcggat tacctacaat agttgatttt aacccaaatg tgagtattgc agtgggtaat
    12001 tattatgtag gtatgaattt tgcctataat acaggcgata cattagctat tattaccaac
    12061 agagacggaa acactgtacc cggtactggg tatgagcaat ttgataccgg aacatggtat
    12121 gcttattcaa acaccactag ctcatggggt ataaatgttg cacatgctat tttcccaatt
    12181 gtttgtacat caacaggaat aagagaagtg atgacaccgg gcaataactt aatggtgttc
    12241 ccaaatccat caaacggaga gtttactttg gttgtaccac aaagcgacct gaaagaaaat
    12301 gttgtggttc gtgtaattga tgtaaaaggc gcagtagtac taaacaaaga attaatatca
    12361 tcaggtaatg gaacctatcg tttaaaactt gatgctcctg ccaaaggcat ttacatgctt
    12421 gaggtgcaga cagtaaatgg tttgaaaaaa caacgtatat ctgtgaatta aaaaacacaa
    12481 tcattaataa aaaaagctgc ttcaaaaggg cagctttttt tattgctgtt ttatctagtc
    12541 ctgaaataac cctaacaata ctttcatatt aaagaaacgt tctgttttta tatttgtatt
    12601 caaattttca gatgaatgtt taaacgaatt ttaatcatcc tgtttgcatt cgcaacggct
    12661 acggcatttg cgcagaaaca aacttataca tcaacttctt caagggctgt taaaaatctt
    12721 gaacaagctt taaaatatta tgattcgagg cagacagcca aagcaatatc tgaaacagaa
    12781 aacgccatta aagccgaccc tgcttttata gaagcctata tgctgcttgg cgatttgcaa
    12841 gcagacaaca ataacatacc tgcagcaatt gaagcttata aaaaagcaat tgccataaat
    12901 ccccggtttt ttccaaacaa tttttatgca ttaggcagat acgagctgga atcaggaagg
    12961 tatgatgatg cattgactca tttcaatgaa tttgtaaaaa tggaaacagc gaaagctgac
    13021 aagaaagaag aagcaaaaaa cctgattgcc tcctgtgagt ttgctaaaaa agcaatacaa
    13081 aatcctgttc ctttttcacc ggtaaatgca ggtgcaggca taaattcaga catggatgaa
    13141 tattttcctt caattactgt ggatagtaaa cgtatgttat ttactcgcaa gattaaaagt
    13201 gatctttatg aatggcagga agatttttat atcagcacat tacaacaaga cagcacttgg
    13261 ggcaaggcaa aaaatgtggg agcacctgta aatacagcgt taaatgaagg cgcaccatca
    13321 tacagtgccg atggccaact gatatttttt actgcatgtg acagacctga aagtaaaggc
    13381 tcatgtgaca tttattactc gagaaaattc ggtgatgcat ggggaaagcc aatcaattta
    13441 ggctctccgg tgaacacggg cgcatgggaa tcgcaacctt cgttttcgag cgatggcaag
    13501 acattgtatt ttgttcgtgg cagtaacagc cgcaccaacg atcaggatat ttatatgagt
    13561 gaagtgagtg aaaaaggatg gagtactccg gtgaaactaa acaacaacat caacacagca
    13621 ggaaaagaag aagcagtgtt tatacacccg gataaccaaa cgctatattt tgtttctgac
    13681 ggatggccgg gcatgggagg ttcagatatt tttatgagca agcgtcaggc tgatggtcag
    13741 tggggcaaac ccataaacct tggatatcca ataaacacat ttgccaatga agcaggatta
    13801 attgtcagcc ctacgggaga taaagcctat ttcagcagta acagagaagg tggattgggt
    13861 ggcttggata tttacagttt cgacttatat gataaaatta aacctgaccc tgtgagttat
    13921 gcaataggat atgtgtatga tgatgaaaca aaaaaaccta ccgatgcaaa atttgaaatc
    13981 attgacattg aatcaggtaa aaccgttgta gaatcatatt cagacaaaaa cacaggagaa
    14041 tttatggtga ccttgcctta tggaaaaaat tatatgctga atgtttcaaa aaggaaatat
    14101 cttttctact ccgatcattt tgaaatggat aaacttgctg acattaaaaa tgcaaagcag
    14161 ttgattgtaa gacttaaacc tgtgaaagac ggcagtacca tgtcattgaa taatattttc
    14221 tttgacacca actcggcagc actaaaaccc gaatcaaaaa cagaactcaa taagttagtg
    14281 agttttataa aaagcaatgc aattgtaaaa cttgaaattg gaggacacac tgataatgta
    14341 ggtgatgaca aagctaatat gatactgtct gataaacgtg caaaatcggt ttgtgattat
    14401 ctaattactt caggtattga agcttctcga ttggtttcca aaggttatgg agaaacaaaa
    14461 cccgtggcag aaaacaccac tgaagaagga aaagcaaaaa acagaagaac agaaatcacc
    14521 atattatcag tgcattaatt ttttttgcat acaaaatttt tctgttaaat acgtgttgaa
    14581 aattatttaa aaatattttc gaagcaaggc gccttgcgaa tgcctaaatt tgcttcatgg
    14641 aagaaaatgt ggaagcaggc aggagtgcaa aaaagaaagt agctcccaag tcgttaacaa
    14701 atcaacaaat tgcattagga ttaggcaaaa tcccaccaca ggctgtggat atggaagaag
    14761 cagtattagg cgcattaatg cttgagaaaa atgcccttac agctgttata gatgttttac
    14821 atacagaagc tttttataaa gaagcacatc agaaaatatt taaagtaata catgaacttt
    14881 ttcataaagc tgagcctatt gatattttaa cagtgacaca tgctttaaaa aaagcaggtg
    14941 atttagaatt agtgggtggt gctttttata ttacacagtt gaccaacaga attgcttctt
    15001 ctgccaacat tgaatatcat gcacgcatca tacttcaaaa atttattcag cgtgagttaa
    15061 ttcgtatttc gagcgaaact ataaaaagtg catacgaaga cacgtcagat gtattgaaga
    15121 tgcttgatga agcagagaag aatttatttg caattgctga aggtaacatt cgcaggaatt
    15181 acgaaaacat gaattctctt atttccaaag ccattaagga aatagaaatt gcatcaaaac
    15241 aaacacaagg tattacaggg gttgaaagcg gcttcagcga attagacagg ataacagcag
    15301 gatggcaaaa atctgatttg attattgttg ctgcaagacc cggtatggga aaaacagctt
    15361 ttaccctatc gttagcagcc aatgccgcaa tacaattcaa caggccggtt gcgtttttct
    15421 ctctggagat gtccagcgtt cagttggtaa accgtttgat ttctgcacag gcagaaatct
    15481 cttcggagtc cataagaaaa ggacaattag aaccctatga atggcaacag ctaaacagca
    15541 aaattgtacc actttctgat gcacctattt ttattgatga tacgcctgca ttatcggttt
    15601 ttgaacttcg tgccaagtgc agaagacttg ttgctgaaaa gaaagtacaa ctcatcatca
    15661 ttgattatat gcaattgatg gtggcaggaa cagacaatcg caatggaaac cgtgagcagg
    15721 aaatcagtac catttcgcgc tctttgaaag cgatagctaa agaattggaa gttccgataa
    15781 tttgcttgtc gcagttgagt cgtgaagtag aaaaacgcgg aacagcttct aagcgtccgc
    15841 aattgagcga ccttcgagag tccggagcaa ttgaacagga tgctgatatg gtaatgttta
    15901 tttaccgacc ggaatattat ggtatggatg tagatgaaaa tggtgattca acatcaaata
    15961 ctgctgaaat aattattgcc aaaaacagaa atggctcttt aaacaatgtt aaactcacct
    16021 ttattggtca attcaccaag tttaaagatc ttgaagtaaa gcgacaagaa gatccttctt
    16081 atcattatac tgatgaaagc agtaaggtta gaatgcctaa tccgaatgtt gaagcagaaa
    16141 atcgttacac gcgtggttca agaatgaacg acatgcctga tgaagatctt gtatagtctg
    16201 tcttaatttt ctaattgctt ctttacaatt tccaaacctt gctgcaatgt gtgaggccta
    16261 aaatttagtt cacgttttgc cttatcaata ttaaaacctg ttagtaaagg acgttttgca
    16321 ggttgattga gttgagccgt agtgatggga tgaatatatt ttttatccaa ctgaaaaaaa
    16381 tctgctgtac ggtttaccat atcgagaata ctaattgttt cacttccgga tacatgatag
    16441 attcctgttg cacgttttaa tgcagcctgt acacatgcat cggcaaggtc ttcggcaagt
    16501 gttggtgagc gaatctgatc gctgataaca ttaatatcct tttgctgacg cagtgaattt
    16561 attgtccaga taacaacatt gcttcgctgt ttgtcgtcta ctactccata aatgatgatt
    16621 gtacgcaaaa cagcagcatc tattttagcc tgttgaacaa ctttttcaga ttcatatttg
    16681 ctgcgtgcat aatgactcaa cggattggga acgtcttctt cgctgtagtt gcctttttcg
    16741 ccatcaaaaa caaaatcagt actcagatga ataaaatgta tgttgtgccg ctgacataac
    16801 tcagtcattg tttttacagc ttcaacattc atcttccagc agttctcctg atctttttcg
    16861 caggcatcaa cattggtcat tgcagcggtg ttgatgatac aatgcggctt aaaattctca
    16921 acgatggcgg ttacttcatc agcgttggta atatccattg aagcatattg atagcctgct
    16981 ttagcatgta atcggttttc gcctttagaa gttgcgagca gttgaatgtc atttcgtttt
    17041 aaagctgcat aaacaatttt ctgccccagc aatccattac ttcctgtcaa caaaatacgc
    17101 agcatgactt tgcaaaatta aaacgttaac gatgcaccca atgatggcat tattggcaat
    17161 tgatttacta cattgtgtct gatacggtca acataaaaca cgtttttacg attgtaaaca
    17221 ttgacaacgc ttgccaccac ttccaactcg ctgtttcgta caagatagaa aattttcttg
    17281 attgaaatat caagacggtg gtaggttggc aatcgcgatt catttaatgg tccgtaaagt
    17341 acacccattt cgccattgtt ctgtgtatag tcagtagcaa gtccatcctg gaaattaaga
    17401 taaggataaa agccctgatt taaagtaaca ggaaaacctg atccgaagtt ccagcgtgca
    17461 tcaacttccc agtcaagttt tttacctaac ttatatgagc cgatgatatt gatattgtgt
    17521 cgtctgtcaa aatgcggagc gtattcatag ttgcctgtat tgcgtgtgct gaatcctaaa
    17581 gaataaacaa gccatgcata cattcttctg tattcatact tcagtactac gtcaacaccc
    17641 tgtgtaaatc ctttttcgat gataaaactt ttcttaacgg agtcgggctt gtcgaagttg
    17701 tttgcggtat catcataaac cttgtcacgg ttgatgttgg taagttgatt aaaatcttta
    17761 atataacctt cgatgttaat atcaaaatac ttattgatat catattcaac accaacaaca
    17821 gcatgacggg caagctgcag ttttttctta acagactttc catcaaactc agaaggtaaa
    17881 tcgtcaggag cagaaaggaa accatagaaa aaatttacca catcgcggtc gctgttggca
    17941 gcaatcaggt tttgtgcata cagcccaccg gcagccttaa gtctgatgtt gtcgctaagg
    18001 ttgtatttaa gtccaaggcg tggctcaggt gaaaactcag agatggatgc ataatactgc
    18061 aagcgaacag atggatcaat gatgagttta ttgatgattt ttttgtagcg aaaaaagatt
    18121 cctatttcag tagtgttttc tgtctgattg atttgcctct tggcataatt aaaatattcg
    18181 aaatcggttt tgaagccttg cacttctata ccataattaa tttcgtcatt atttaaaaag
    18241 taaagccagt tgaagcccat gttaaatcca ttcactaagc tggaacgggg tttttcctca
    18301 gcttctgtaa gtttaatctt atagctggag aaagcgaggt taccgcgcaa caatactttt
    18361 gatgcaccgg gcaccaacac aaagttcatt ccaccaccgg aagaactcca gtcgtatttt
    18421 gctaccttgc tgtaatttac tttatcaaga aaattaaatc caaaaaagct aaccttactt
    18481 ccgctggcac tgttgattac cacttttcca taaatatcat tgaaagaata tggcagtccg
    18541 tcatcggaag catatttgta aatacttttt gaagtctggt cgaggtagga gttttttact
    18601 gaaagaataa aggaagctgt tccgtcattt tcgtcttttg ctttctttat cggtccttcc
    18661 aacaaaactt tagaaagaaa tgtgcctgta gaaacttttc cgccaatgcg ttttttattt
    18721 ccatcgcgtg tggtgatgtc catcactgat gaaatacgac caccatattg tgcaccaaaa
    18781 ccagctgtat aaacatcggt attttttaaa atgtctgtat cgaacacact aaacaaacca
    18841 atgctatgaa acggattgta gatgaccatg ccatcgagca gcactttgtt ctgcacaggt
    18901 gttcctccac ggatatacaa ttgtccaccc tgatcgccac tgcttacaac accgggcact
    18961 acctgcatgt actgcaccag atcgccttcg gcaccaagac ttggaagaga tttaatttcc
    19021 tgaggtgtta cagactctac tgcaatttta acttcagtct tttttgtttg tctttctgct
    19081 gacacttcaa cctgcttcaa ctcgacattt tttttcttca tcaccaattt gcggttttcc
    19141 atttcatcag caacaagttc aacatctgca aaagctgtgt cgtagcctaa tgttgttgcc
    19201 ataacgcggt actttccggg aggaattttc accatggaat aataaccgtt aatgtctgta
    19261 aatgatgcaa aggttgttcc cttcagaaaa acacttgcaa agggaacagg ctcaccgttt
    19321 tcggcagcat aaacaaaccc tttgatggtg gctgtttgtg catttgccag agaaacggca
    19381 agcatacaga aagcagctaa aaaaattctt tttatcgtca taaaaactat ttacttaatt
    19441 ctaaaattac attaaactct tctttcttta gtggcattac cgacaggcgg cctatgcgca
    19501 ccaatgcaat atctttcagt cgtgcatctt cttttacctg tgctaatgtt acaggcttct
    19561 tcaatgcttt aaatggtgtc aactccacac ccacccaacg tgcatcgtca gcagtggggt
    19621 cggaatatgc agttttttcg actttagcaa tacccataac ggcaagacct tcgttgctgt
    19681 gataaaacaa aacctgatcg ccttttttca tggcaagcaa atgcaggcgt gcagcgttat
    19741 tgcgcacacc atcccaaaat gtttttccat cggcaacaaa ttgctcccac gaatatttaa
    19801 acggctctga ttttaccagc caatacttca tactttttct aaagtcggac aaagaaataa
    19861 aaataaatga atccggattt gttaattatc cttaaataaa ccctttgcta attgttcaat
    19921 tacaggtggg taatgctcat attcaaggcg atgaatacgt gatgctatgc tctctgctgt
    19981 atcatcaggt agcacaggca tacttttctg aaaaataatt tctccctcgt caaagcggct
    20041 gtttacgtag tgaatggtga tgcctgtttc tttaacacct gcctgcaaca ctgcttcatg
    20101 aacgttgtgg ccatacatgc ctttgcctcc gaaaagtgga agtagtgcag gatgaatatt
    20161 tatcatgcga tgctgaaaag ctgaaacaac ttcggcaggc atcaatttga ggtagccggc
    20221 cagcacaacc aaatcaatgt tatgttgctt tatcaaatca agcatcagac gaccatcact
    20281 gatagttttt ttatccataa aaaaataagg aatatttaac ttctgtgcgc gtgctatcac
    20341 acctgccgtt gccacattgg taagaattac tgccacttct gcatattcat tattcttaaa
    20401 ataagcagca atattttcgg cattggtgcc cgaccctgat gctaatatgg ctattcgctt
    20461 tttgttcatt gcacaattat gagcgtgtaa aaatagcatt ctgtaaatga tgggcttaga
    20521 tatttttttg accttattac aatcattcat ttggttatta tatagcctgt atattaaatt
    20581 tgtaggaaat tcattaaacc acataacatg tatttctgca ccaacataaa gctacttcgc
    20641 aaacgcaaaa agcgtacaca ggaagtagca tcacttgcct taggattttc gcgctctaca
    20701 ttaaacagtt atgagaatgg cgctattgcc aatccaacag ttgaggcatt gataaacttt
    20761 tctaattatt ttaaaatttc tatagacacc ttgctgcgtg ttgacatgag taaactcagc
    20821 gacttacaac tgcgcgaact ggagttgggc tatgactcct atgtgcgtgg cacaaagctt
    20881 agggttcttg ccacaacggt tgacagcaga aaccgcgaaa atattgagtt ggtgcctgtg
    20941 aaagcaaaag ccggatacac tgcaggctac aacgacccgg agtttatacg caatcttcct
    21001 acatttcaac tgcccttcct ttcgcacgac agaaaatacc gcacctttca gatcagtggc
    21061 gacagcatgc tgcccatacc ggacaaatcg tttgttacgg cagagtttgt agaaaactgg
    21121 atggagataa aagatggcaa tgcctacatc atactcacac acgatgatgg cattgtgttt
    21181 aaagtagttt ataatcacat cagcagcaat aaaaaattat tgcttaaatc actgaatcct
    21241 gcctataaac cttatgaggt aaaaattaat gaggtaaaag aagtgtggcg atttgtaaac
    21301 tatatcagca atgaaatgcc tgatacggga ttacaaaatc caaaactatc taacactgtt
    21361 gcacgcattc aaaatgaaat gaataaaatc aaagacctgc tgggtgtaca ggatgttagt
    21421 aaagaatgat ttttttgagt gagagcgtat tttgtacttg caaagttgtt cttccggatt
    21481 tgaatacaaa cagattaata atctaactac aaaaggcttt gggatttaat atacctgatt
    21541 ataaaatcag tattttcaca taaccacgaa ttgcaactca gcagggatta aagaatattc
    21601 tattctttaa taaatttcgc tttgaagttt tcattattac ttttaaaaaa ctcaatgata
    21661 taaaccccat tagaaagcgc atctacattt agtttgccgg acattaattt gctatgtata
    21721 atttcacgtc cggcaatatc ttttacacta aaggtataaa agttttttaa aatattttca
    21781 ctgaaataca gcactttttt tacaggtaca ggatatacaa aatattgtaa ctcctcactt
    21841 ttaatttctt tgatcgttgt aatagcagga gcattaaact tcaaaacaaa cacattaaaa
    21901 ttcccaaccc cgttaaacat taacgtctga actaaaggag aattattaaa atcagctgta
    21961 tcactaaatt gccctgctac ataaacagca tcgtctgaag cacataatgt cgttgacctg
    22021 tcaagtaaat ttccaccaaa actttcaccc catgcgtaat ttcctgtatt ggtgagcgct
    22081 acaacatagg catcataata atcatcttgc ggagtagcag ccacttcata taacatgttc
    22141 actcctgctg gcatattaac atccattgta tcagtaaaca ctcctccaac atataccaaa
    22201 gtatctttta ccaatatagc atgagcatga tttgctgcca ccgataaatt agtgctgttt
    22261 tgagtaaatt gtctaaccca aaccaactcc acgctactat tcaaatggca aacaaatgta
    22321 ttgtaagcac caactgcatt tacataaaaa gccgttgtac tatcggtgtc aaaatctatt
    22381 gtgtcattga agtaaccaca tacaaacaca ttacctaaat aatcacatga taatgatgta
    22441 acattacaat agccctgagt acttaccgga ttattgttga atgatttctt aaaaacaaaa
    22501 tttcctgctg tgtcaacctt tgcaatttca ccgtagttag caatatagat attaccatta
    22561 tgatcataac cggctgccat acgttcatac tcaccataat aactcagttg agcggcttgc
    22621 acccaaacat actgccctgc actatcatac tttgcaatat acacactttc cggggtgctt
    22681 gttgaaagat ttgccacacc cgaaccagga tcaaaatcac atgtattatc ataacgtccg
    22741 gtgatataaa tatttccaga ctgatcaacg accagatttc ctgctgactc gaaccctcct
    22801 gttccaattt tgaacgccca tataaagtta cctaaagaat ctaacttaag gacaaatata
    22861 tcagggttac ccgaagaagc atttgttgca gtgagtgtat aatttataag tgtgtccgga
    22921 ttaaaatctg ctgaggaata aaacagccct gcgatataaa cactctgtgt actatctgtt
    22981 gccacacccc aagatattac atcactgcct acaatacttt tagcccaaat aagattaccc
    23041 agactatcca ttttagtaat gtaagtatta tttccacctg ggttgaaagg caaataaaca
    23101 tacccggggc cgggatcaaa atcaacatta ccaataaatg aacctgttgc ataaatattt
    23161 tggtgagaat cgccacacat ggcatttaca tcttgagcac caagttgaac agcccactgc
    23221 gtttgctgtg cccatgattt ttgcaatgtg agggataata aaattattat gccagaaaac
    23281 agcagcaatt taattcttaa aacagtcatt tgattctgga taattatatt ttcaaagtta
    23341 gtcaacaatc tccgcttttg aaaaatattt taggtgactt ttctcataca gtataaagat
    23401 taacattgcc tatctttatc tttttggcag aaaacataaa aacaacttac ccactaaatt
    23461 tatatttatt aaatcagtac agagtttaat tgcaccatca cttggtagtt tcacttttca
    23521 tctgctcacc caacttttca tactctgtat cattcggctc ccatagttca attttatttc
    23581 cttccggatc gagtatatga acaaatttac cataatcgta ggtttctatt tcatcaacaa
    23641 tagttacacc aacgctgcgc aattctgcaa ctaatgcttc tatgttttcc actctgtagt
    23701 taatcataaa ttcctttgat gaaggttcaa aatatttagt tttatcatca aacgggctcc
    23761 atgttgtaaa acctttctta gagttatctg ctccctgata tcattcgaaa actgctccgt
    23821 aaggattggt ttttaatcca agattctgtt ggtaccattc ccgcattttt ttggggtctt
    23881 tacatttaaa aaaaatacct ccaatacctg ttaccttttt cataattatt tgatttgaat
    23941 tttaagaata agctttttca acctattaca ttcaacaact gcatcacgac ctgaagttga
    24001 catattgtaa cggcaactct acaggcttgt tttttacttc ggcaataacc tgttgcaagt
    24061 catcaatttt tttacctgta acgcgcacct gttcatccat gattgacgcc tgcaccttca
    24121 ttcctgtttc tttaataaac ttcacaatgg ttttggcagt ttccttgtca attccttttt
    24181 taattttcac atccttgcga accatattac cggaggcata ctgttctttg ccaaaatcga
    24241 aacacttggg gtcaacaccc tgcttcatac ttcggctgat gatgatgtcg cgaatggcat
    24301 ccatgcgcat ctgatcttcg gtaatgattt ttaactgcat ttctttcttg tctaaatcaa
    24361 tttctgtttt agaaccgtga aaatcgtagc ggttaacaat ttccttcttc gccacattaa
    24421 tggcattgtc taatttctga atgtctattt cattgacgat atcaaatgat ggcatgttta
    24481 attctttttg ttcatgcaaa agttgtaata attcatgaat attctgatta tgacggcaat
    24541 ttttttctac ttttgaatgt ccttatcagg gtcatgactc aatatcagaa aatgaaaatc
    24601 aacatcatca gccatgtttt acgacatgcc atgctttgcc ttaccgtgtt tattactgtc
    24661 aatagcgtct ttgcacagaa ctacagcaag tacacttcgc caaaggtatt tgtaaataat
    24721 caggcattaa aaaacccctg gtcaggtgga attaactctc ctctttacag cactattgac
    24781 ttaaacaatg atggcataaa ggatttgttt atgttcgata aacaatttgg tgcagcttat
    24841 ccgggctcct ataaaatcag aacttttatc aatcagggaa ctgcaggaca agttgattat
    24901 atttatactc cattttacga gcgttttttc ccacaaaacc tacatgattg ggtaatattg
    24961 gttgactatg attgtgacgg caatgaggat atttttactt actcctactc aggaggtatg
    25021 gaagtttacc ataacgatat cacaccgggc aacaatcttt ctttcagctt aacttatcct
    25081 cttattcata gtttattttt aggaagtgcc tatgcaaacc tttatgtgag caacgtaaac
    25141 cttcctgttt tgcatgatgt aaacggtgat ggcgatttgg atgttttaac attcacctta
    25201 cttggcggca ctatagagta tcatgaaaat tatgccaagg aactttataa ccgttgtgac
    25261 actttagtgt atcgtcaggt gcgacagtgt tatgcacaag taggcttaag tgctgtttcc
    25321 aacactgcaa ttctcgattt taataattgt ttttttctgg caccaccacc actacccgat
    25381 tcgatgataa aaacacctcg tcacaatggt tcctgcatga taggatatga tcaggatggt
    25441 gatggtgatc ttgatttgat taatggcgat attcttggca acaacttgct ttatcttgag
    25501 aacagtggta ttccgggagt gagtgattcc attcacttac aggacacact ttttccatcg
    25561 tataatgttc ctgtaattta taaaacattt cctgcgccat tttattttga tgccgataat
    25621 gatggtgcga aagatttaat tgtttcaagt tgtacagagt atctgacaga agattatcaa
    25681 aattcctggt tctataaaaa tgttggctca aacaccaaca atcagtttca atttataaaa
    25741 aaacgatttc ttactgatga aatgattgat gtaggttcag gggcatacgt tcgttttttt
    25801 gatgtggaca atgacggaaa aaaagatttg ataattggca actacggtta ctttactcca
    25861 gattctgcaa gtggacatta cgaatctgca ttatcatatt atcataatat cacaacaggt
    25921 ccactacctg aatttgaatt tgtgacggat gattttaata acctgcgttc attaggcaga
    25981 accgggttga atgccacttt tggtgacctt aacggtgacg gtcatgatga tatgatttta
    26041 ggagataaag atggcatttt acattactat cttaataacg gtacaacacc tgcaactttc
    26101 acacttgcac ctaatggttt taactttcaa aatattgatg taggcacaaa cagctttcct
    26161 tttttagctg atataaataa tgatggactt ttagatctta tcattggtga acgttacggt
    26221 tacatttatt attatcagaa tacagggtct gcaaccaatc cggtgtttac ttatgtaacc
    26281 aataaattag gcaatgtgtt tgttggaaat gcgcaatcgt tttacggatt cagctatccg
    26341 catttttata aagaaaatgg tgtgtcgaaa cttttggttt caaatgaaaa cgggtacatt
    26401 cagaaatacg ataacattga cgggaatctt ggcggcacat ttaatcaggt ttcaaccggc
    26461 atgttttcag tatttgaacc ttatcagact tcattcgaca tggccgacct aaacggtgat
    26521 ggcatggatg aagttataac aggatgctat gccggtggcc tatccattta ttcaaaaaac
    26581 cttcccgtag gtatagcaga aaatatacat cctgaaaaag aaattacagt ctatccgaat
    26641 cctgcaaaaa attatcttgt ttttgagatg aataaaaaca atgcacataa cggcaaatat
    26701 caattatccg acatttcagg caaggtagta ctagaaggct cttttcaaag ttacattaat
    26761 aacataagtc ttcaaggatt aacaaaaggt atttatattc ttcaggtaac tgagagcaat
    26821 catttcagtt cacataaggt tgttaagttt taatatgaaa tttttttctt tttttctgtt
    26881 ttcattactg attgttcatc aaacatcaac atcgcagaca tataataaat acacatcacc
    26941 tgtggttatt aaaaatggtc agccactgcg aaatgcatgg gccggaggtt ttaactctgc
    27001 catgtttcag gctattgaca tgaacaatga tggtaaaaaa gatttgtttg cttttgagaa
    27061 acaatcggag tcaacctatc ccggagtttt taaaataaga acttttatta atcatggcag
    27121 tgcaggaaat actgactatc gttatgatcc tttttatgaa aaactttttc cacaaaacat
    27181 gacaaacttt tgtttgcttg ttgattatga tggtgatggc cgcgaagata tttttacata
    27241 caaccgttcg gtttccggat ttgatgtgta ccacaacgac tctaatgtta caggagtatt
    27301 gacatttagc ttagaatatc aaggattaca aacttgttat ctgaatgccg gaggcaattg
    27361 ctttacaaca agtctttacg tagcaccggt aaatcaacct gtattgttcg acatcaataa
    27421 cgatggcgac cttgatgttt tgacatttca gatttctgca aactttattg agtatcatga
    27481 aaacatggca aaagaattat tcaacagaac agatacacta gttttgtctg aagttaattc
    27541 aaagtggggg cacatgtcat tgagtagtaa tgccaacagt gctatccttg gtatgccaag
    27601 aattccttta acaaatggca atgcagatgt tttatccaat cgccattccg gaacatgtat
    27661 gattgcattt gatgatgatg gcgatggcga tgctgattta ttgaatggag atttattagg
    27721 tgacaattta ctttatctgc ataatggcgc aaccaataac ggcacagaca gtattgactg
    27781 gcaggacaca tcatttccaa caaacggtgg tccggtaaat tatgtaacat ttccgagtgc
    27841 ctatttgttg gatgcagata atgatggtat aacggatatg attgtttcgt catgtacaga
    27901 gtcgcaagca gagaacctga ataacaactg gtactatcac aatgccggca gcaataccgc
    27961 tccggatttt caatatgtaa aaaacaagtt tcttaccgat gaaatgattg atgttggcac
    28021 tgcttcaatg cctgtatttt ttgatgttga tgctgatggt aaaacagata ttgttattgg
    28081 taacaagggt tattttatta cacaaagtgg acctgcacac tatgaagctg cattgtcctg
    28141 gtataaaaat accagtaccg ggccatgtcc tgaatttaca ctggtatcaa gcaactttgg
    28201 ctcctttcaa caatacaatt acaaaaacat ctatcctgcc tttggcgacc ttgatggcga
    28261 tggtgatgct gatttattac ttggcaatga agatggcact tttcattact acaccaacac
    28321 tgctggcgtg ggaaacattc ctcaatacac actgacatcg ggtggattta attttcaaaa
    28381 cttagatgtg ggcagtaata gcacacctca gctttatgac atcaataatg atggactttt
    28441 ggatttaatt tccggtgaat tattcggaaa aatatcatac ttccagaata ctggaaccgt
    28501 tacagcacct atattttcgc tcataacgcc tacattcggc aacatcaatg tgacaaacac
    28561 agcacaaaca ttttatggct acagcatacc ccacttattt tctgacaatg gctctttaaa
    28621 gttactggta ggatcagaaa gtggagattt gtttttatac gatagcataa gcacaacagc
    28681 agggagtact tttcatctga taagtaataa tgcatactta attcatgagc caccacgtgc
    28741 tgcacctgca atggccgacc tgaataatga tggctctcct gaaattattg taggcaatca
    28801 ggcaggtgga ctatcatttt attcaaattc attaccggga tgtccacttt ccatcaataa
    28861 tatattgtca gataatcagg ttaatattta tcctaaccct gccaactcat atttgataat
    28921 tgacacagat gatgccgatg gcatgaaaaa actaattaca ctgaccgatt tatcaggaag
    28981 aattattcat tcacagaaca ctttttcgcg taaaacatta ttaaatacag aaggtttagc
    29041 caatggtatt tacctgatta tattaactgc gcaatctaat aatattacaa aaaaaataat
    29101 catccgtcat gactaagaga tattttttat ttacaacgct ttgggcattt acgctgtttt
    29161 tgacatcatg cacatgggat aaaatggatg aagtggctcc gccaacagac agttgttcgc
    29221 aatatccacc tgaaatttct gctatcatca gcaacaaatg cgcaacagca ggatgccata
    29281 atacactttc ctattctgct tcaagtggac ttaatctgac caactgggct tcaatgataa
    29341 aaggtaatga tgcaggtgca gtggtgattc cgggcaatgc cgattacagt acattatttt
    29401 cattctgcaa tatttattcc gacttaggat ttacaggaaa cgaaccaaag atgccataca
    29461 atgaaactgc tctctcacac agtgaagtct ctactatcaa aaactggatt atcaatggtg
    29521 caaagaatgc ctgtggtcag gatatttttc cgcaagacga caacagaaaa aaagtttaca
    29581 tctccaatca ggcctgtgat caggttgcag ttcttgatga agcatcagga ttattgatga
    29641 aatggtttga tgtaggaaat attgcaggct tcactgaagc accacacatg attagactta
    29701 caccagatga aaaatatttt ctgattgctt tttttagctc aggcatcata caacgctaca
    29761 gaacttctga cgacagtttt gttgatcagg ttgatttcag taatggcatg ccgggacaat
    29821 ggggaacctt ggttatttca cctgacagca aacgtgctta tagtgttgac tggagtgcat
    29881 caggcagaat tgctgtgttg aatcttgaaa actttccaat gacatcattc acttccttac
    29941 ccatcataaa tttagcatac ccacacggta tggcatttaa cagtactggc gataagcttt
    30001 atatcaccaa tcaaaatgga agcacatatt acgttgtgga tacgattgga agcccatcag
    30061 attttccgca atggctcagt aatattcaga cattttcata cggtggtgga cttaacgggc
    30121 atgaaataat tttctctcct gattattcaa aatattttat cagttgcgat gctactaatg
    30181 aagtgcgcgt ttttgatgcc aacactcatg cattgcttgc aaatattcca gtaggcacat
    30241 tgcctcagga gttttcttta tcactcaaga aaccctattt atttgttaca tgtactgaag
    30301 atattatcaa tggaaaacgt ggttctgttg ctgtgataga ttacaataat ttaaccttaa
    30361 tcaaaaaaat acatacaggt acacaaccac atggtgttgc tgttaatgat gataaaggtg
    30421 ttgtttatgt tgccaacaga aatgttgatg ctaatggtga agcacctcat cacagtggtg
    30481 catgcgcagg gcgaaacgga aatctaacgc aaatagatat gaacacactt gaattggttc
    30541 cgggatataa atgtgaattg ggtgtaaatc catattctgt tgcttacaag aaataatcag
    30601 ataacttctt ctatactaaa tcctaaagat ttcatctgct caaaataatc gggaaacgat
    30661 tttgagacat ttgaaatatc gtcaagaaat agctctcctg tctttaatgc taaaggtgca
    30721 aatgccatta tcaaacgatg atcgttgtag gtttttaaaa tgcgtttgga aggttttaag
    30781 tttgactctt caagaatttt taattttgat cctccacaaa aatcagtctt gatgttgagc
    30841 ttatatgctt cacgctgaaa ggcagaaacg cgatcagatt ctttaattat taaattatgt
    30901 agatgaagca aatctgcttt acaatcgcat ccaccacaaa cagctgccat acttaaagct
    30961 atgtcgggat agtgaagaaa actatgtgta aaataatcag gaataatatt cttttgtttt
    31021 ttcagaataa tcttatcctt ctcaacaatg gtatccacac caaatgactt aaccatttgg
    31081 gcaattactt tatcaccttg cttactcaga ctatttagat tttcaatggt tatatcagcc
    31141 atatcagcca atgcagctgt ttcaaaaaaa tatgaggcag cactccagtc gccaccggca
    31201 gaaagtatgt tgttgttgta acttccactt atctgaatac gatttccggt catctgcaca
    31261 tttacgccta cctgctgcat caaatcagca gtaagttcaa tatatggtaa tgaacttaaa
    31321 atgccggtca gttcaattgt aaaatcttta ccaaacaaag gagcaatcat catcaaagca
    31381 ctaatatgct gtgaagtttt ctctgctgat gtttgaacta catcaggcac tttcagtttt
    31441 gcagcctgaa cgctgacagg cagaaatcct tcttcctcta aataagaaat ctctgctccc
    31501 aacactctga gcaaatcaac agaacaactc attgggcgtt gacgaagtcg tgggcttccg
    31561 gtaattacag ccgctctgtt cctgacaaca cagtaagcca taataaatcg tgccgtggtt
    31621 ccaccatctt gcgcatctaa cgtatgctct tctgaaaaaa gcaattcctg cagcttgact
    31681 gtatcagttg caaggcaatc attattctgc gcaactcctc taagcgcatt gatgatggca
    31741 atccgacccg cctcgctttt ggaaacagga acagcaacta ctccttcaac acttctatca
    31801 ggctttgtaa ttctatatgc cataaccagg attttgctta gaaaaccaat gaaaagcatc
    31861 ttcaatcatt gctatcggaa caggaatgtc aaccacacct ttaccaattg attcaagtaa
    31921 tgtgaaacgt aattggccct gcttgttttt tttatctgcc atcataaacg gcaacagttt
    31981 ttctacatta aaatccttat catcaaactt aaacaatgat tgaacaaaac cggtgatttc
    32041 atttaacgct gcttctgaaa taaaacccat tttgcaggag agataggatt caacaataat
    32101 tccaacagca actgccttac catgcgaaat attttgccgt tgcaaatgat acatagattc
    32161 caatgcatga gcaatagtgt gaccgaagtt gagcaacttg cgttcattta tttcataagg
    32221 atctgatgca acaatacttt gttttattgc aatagctttt tcaataattt gcaacgcttc
    32281 aaaattgcgc tgttcagaaa gtgatttata aaatagttta tcagcaacaa gagcatgttt
    32341 aatcatctct gcatagccgt taactttttc aacatgcgga agtgacttta aaaattctgt
    32401 gtctatataa acatatcttg gtataacaat actcccgctt tgatttttaa agccatcaat
    32461 atttacccca ttcttacctc ctatagctgc atcaaccata gccagcaagg ttgtggggac
    32521 atgaataaag tcaactcctc ttttaaataa ggaggctgca aaccctccta aatctgtaat
    32581 tacacctccg cccagattaa caatcacagt gtgcttatct gcctgttgct gcaggaggaa
    32641 cttccagaat gccgttgccg tttcaattgt tttatacgct tcgcctgact gtatgcaata
    32701 tacagaggct gattttgttt gtgggaacaa tgtctgaaac aaaggcaaac aatactctat
    32761 tgtattggtg tcgcatagca caaatatatt tcgcttttga ttattacaat cgtgtaaaaa
    32821 atcaattagc tcctgttttc taatacctga caggatgtaa ctactatcgg caaaaccaat
    32881 cacgacatta atgtttttca gttttcgatg aggctctaag ttcaggtttt gtcatctgtt
    32941 tatcctgttg caaaattgat tcttcatgta taaggctata caatctcaaa ataaaatccg
    33001 gtgtcagttg attgtgtaaa cctgtttcgg ttcttgttct tagaatttct ttaaaacgat
    33061 ctaactgcaa aatagccata ttattctgat ctttatattg gccgatttta cgtacaactt
    33121 ccattctacc tgctatcttt tcaatgattt cattatccaa ttcatcaatc agatcgcgca
    33181 aatcgtgcag cttacttagt tcaacagggt ctttagctgt tggttgcctt acaatagttt
    33241 gttttctaag atgactaaaa tcttcgggtg ttatttgctg catagcatca ctcaatgctt
    33301 ttgccggatt taaatgtact tcaaccataa ctccatcata ttgcaaatcg aatgcaattt
    33361 gtgtgagtgt tggcaccatt ttggcatcgc cactaatatg acttggatcg caaatgatgg
    33421 gcacattggg cagtaagcgt ctgagttcaa tggctaattc ccaattgggt ttatttctat
    33481 atgtactgtt ttcgaaactt gaaaaaccac gatgaacaga agcaattcta ctgattccat
    33541 tttgcataaa acgttctata gcgcccatcc ataatttgac atcggggttg acgggatttt
    33601 ttacaaatac cggaatatct acacctttta attcatcagc aatttcctga atgaggaatg
    33661 gatttactgt tgtacgtgcg ccaatccaca acatatcaat accggaagca agagcctgct
    33721 ctacatgttt tgcgcttgcc acttcggtaa taacaggaag tccggaagca tctccagctg
    33781 ccctgatcca ttttagagca ggagcgccaa agccttcgaa ggcaccgggt cgggtgcgag
    33841 gtttccatgc accggcacgt aaaatatgtg caccgccaat cttaagcctg atggccaatt
    33901 cagtcagctg ctgctcgtct tcggcactgc atggaccggc aatccacaat ggctctgtaa
    33961 gcgacttgag tgaatgatga aagttattaa acattagtgc aaagataaag aacacatttt
    34021 atgtgcaggt gaaggcattg cagtgctaaa gagaagacat cacagagcta aagaggatta
    34081 gattaataga tggaaacaaa attagagaag ttaaatttac tacttttgca gcccacaatg
    34141 cccgggtggc gaaattggta gacgcaccat cttgaggggg tggcgccaac aggtgtacga
    34201 gttcgaatct cgtcccgggc actaaaaaaa agttcgttgg tatttgatta ttgcccgggt
    34261 ggtgaaattg gtagacacgc aggtctcaga agcctgtggg gtaaaacctg taggagttcg
    34321 agtctcctct cgggcacttt ttgaggtgcc ggtttagctt tgcttgtttt tttctttgta
    34381 ctatatatat ataaacgcag gcatgaaagc gggcaacaaa gccatcatag cgtttttctt
    34441 ttttttacta agccatagtt gcaaaagcct gattgtttat gattagcccc attgtggtat
    34501 agcgactgca ccgggtttgt gaaatttata tggcgttttg tgaagtggtc gccacgtttt
    34561 gtgaagtggt cgtggcgttt tgtgaagtgg tcgccacgtt ttgtgaagtg gtcgtggcgt
    34621 tttgtgaagt ggtcgtggcg ttttgtgaag t
//
LOCUS       DAOQNP010000024        34614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_403_length_34614_cov_7.662201, whole genome shotgun sequence.
ACCESSION   DAOQNP010000024 DAOQNP010000000
VERSION     DAOQNP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 34614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34614
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_34614_cov_7.662201"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PKY09_05095"
     CDS             <1..1372
                     /locus_tag="PKY09_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB12264.1"
                     /translation="DDLKQRLPMLLGGTDEAAAFYRFMFFSLFSYVSHRIPEIADEIY
                     KIDDAMCAGFGWEIGPFETWDILGVQSVVTQMQKEGYSVAPWVTEMISNGINSFYNID
                     NGKKSYFDLQTKKLNPLPGEGGLIILKNTSSEKIVWKNSGCLLHDIGDGVLNLEFKTK
                     MNTIGSEVLQGINTAIDFAEKDFKGLVIGNEAVNFSAGANLGLVFMLAVEQEYDELDY
                     AVRAFQNTNMRLRYCNVPVVVAPAGLTLGGGCEMSLHADKVQAAAETYIGLVELGVGL
                     IPGGGGTKELTLRFSDSIKPGDIETNALRDFFMTIATAKVSTSAFEAYDLHILRKGFD
                     TITLNRKRLLSDAKNAVLSLANAGYVSPQPRHDIRVLGRQGLGVIQAGASAMLAGNYI
                     SQYDQMLSLKLGYIMCGGNLSSPSLVSEKYLLDLEREAFLSLLGEKKTLERIQAILTT
                     GKALRN"
     gene            1577..2215
                     /locus_tag="PKY09_05100"
     CDS             1577..2215
                     /locus_tag="PKY09_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNB12265.1"
                     /translation="MKILKQPLLLLILLPAFAFAQREEKPRYAPGREKHRNKTDELGQ
                     RQGVWKYYNEAGELNQEIEYLDDVRNGVAKKYYPYGKILEEIEYANGIKDGAYTRYYY
                     NGQAKIEGEYLNGKKSGQWTTYFSDGEIRNSGPYKLNLKNGLWKFYDRKGKLLRQTEY
                     KMGEDVKALAAAKEAEEKAKKADAAKKAAEAKKKLPPGAKLKTPPPATPEKK"
     gene            2269..3444
                     /locus_tag="PKY09_05105"
     CDS             2269..3444
                     /locus_tag="PKY09_05105"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNB12266.1"
                     /translation="MDAYIVAGYRTAVGKAPRGKFKNTRPDDLAVTVIKHLIQSVPNL
                     DTELIDDVIAGNATPEAEQGLNIGRMISLMGLNTVKVPGMTINRYCSSGLESIAIASA
                     KIHSGLADCIIAGGVECMSPIPFGGWRIVPNYEVATNHPEWYWNMGLTAEAVAKEFKI
                     NREDQDQFSFQSHQRAINAIKNNLFKEQIVPVEINETYLDANEKRQTHKSIVDTDEGP
                     RQDTTIEKLNSLKPVFDAKGTVTAGNSSQTSDGASFVLVVSEKMLKTLNVKPMARFVS
                     YAVCAIEPRIMGVGPVYAIPSVLKRAGLKQDDIELIELNEAFASQSLAVIRTLGLNPE
                     ITNVNGGAIALGHPLGCSGAKLSVQLLNEMKRRKMKYGMVTMCVGTGQGAAGIYEVL"
     gene            complement(3451..5061)
                     /gene="bshC"
                     /locus_tag="PKY09_05110"
     CDS             complement(3451..5061)
                     /gene="bshC"
                     /locus_tag="PKY09_05110"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNB12267.1"
                     /translation="MLAQSTYISFTDAGYSTLIGDYLSKSSFFKEYAAFQYDESGLKE
                     ALQKRLQYRTNRDSLCEVLEHQYSETVFQTDWKTSKSYSAIKSLKEEHTFTITTGHQL
                     NIFTGPLYFIYKILTVIKQAEFLSQKNPDYKFVPVYWMASEDHDLAEVNHVSIDESKI
                     EWQTKQKGAVGRMTTEGMSSVVETLKNHLHKNEYFQDIAEMFDTAYLKNATQANAIRS
                     LVHSLFSKYGLVIIDADDKRLKQQAIEMFEDDLFKNTAFNCFKNLSEFEKKYGLQIHA
                     REINLFYLADGERNRIVRNGENFEVADSGLSFASDDLKKQLNAYPERFSPNVVLRPLY
                     QEVILPNIAYTGGPAEVHYWLQLKPIFDKLNIFYPMVVLRNGMLVVNADQANFLNESA
                     LVWKDLFHPTEQVVNIALKRKYVEELSLKVERDAIQRSYAEMEQRFKAIDVTLDKHVK
                     AEWRRAEKRLMTSEKKLLRAFKKKQVDETRKIRNVVEKLFPNGKLQERIENVFPYLNL
                     YGLQFIDDLYSVCNPIPMDFKIALTAKK"
     gene            complement(5154..5636)
                     /gene="rplQ"
                     /locus_tag="PKY09_05115"
     CDS             complement(5154..5636)
                     /gene="rplQ"
                     /locus_tag="PKY09_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNB12268.1"
                     /translation="MRHGKKINHLGRTHSHREALLSNLASSLILHKRINTTVAKAKAL
                     RKYVEPLITKSKSDSTHSRRTVFSYLQNKEAVSELFREVAPKVNERPGGYTRILKTGF
                     RPGDAAETCMMELVDFNELMPSSKEKGTAKKAKSTRRGRKPKAKADAAGSETTGTTEE
                     "
     gene            complement(5662..6654)
                     /locus_tag="PKY09_05120"
     CDS             complement(5662..6654)
                     /locus_tag="PKY09_05120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNB12269.1"
                     /translation="MAILAFQRPDKVIMNHSTETFGQFEFRPLEPGYGITVGNSLRRI
                     LLSSLEGHAITNIKISGVDHEFSTIKGVVEDVTEIILNLKQVRFKKQIEGTDSEKVQV
                     VVNGKTEFTAGDIAKHTAAFQVLNPELVICQMDPSVKLVIDMTIDKGRGYVPAEENKS
                     NSHPLGTVAIDSIYTPIKNVKYAVENYRVEQKTDYEKLVLEITTDGSIHPKEALREAA
                     EILIHHFMLFSDEKITLDTKSKTPVEEFDENTLHMRQLLKSKLVDMDLSVRALNCLKA
                     ADVETLGDLVSFSKTDLLKFRNFGKKSLTELEELVRSKNLTFGMNIQKYMLDKE"
     gene            complement(6719..7324)
                     /gene="rpsD"
                     /locus_tag="PKY09_05125"
     CDS             complement(6719..7324)
                     /gene="rpsD"
                     /locus_tag="PKY09_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018279609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNB12270.1"
                     /translation="MARYIGPKTKIARKFREPIFGPDRYFEKRSYPPGMHGAAKKRAK
                     QSEYAIQLAEKQKVKYTYGMLERQFARFYSIALRKEGITGENLLKLIESRLDNVVFRL
                     GIAPTRAAARQLVGHCHITVNGKVNNISSYILRPGDVVGVRERSKSFEAITDSLASRS
                     NKYGWLEWDTTAMQGKFVTTPAREEIPENIKEQLIVELYSK"
     gene            complement(7371..7775)
                     /gene="rpsK"
                     /locus_tag="PKY09_05130"
     CDS             complement(7371..7775)
                     /gene="rpsK"
                     /locus_tag="PKY09_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNB12271.1"
                     /translation="MAKQQQGAAAKTAKKKVVKVEATGEAHINATFNNIIISLTNLQG
                     QVISWASAGKMGFKGSKKNTPYAAQLAAADCAKTAYDLGLRKVKVFVRGPGAGRESAI
                     RTLNASGVEVSEILDITPIPHNGCRPPKRRRI"
     gene            complement(7826..8203)
                     /gene="rpsM"
                     /locus_tag="PKY09_05135"
     CDS             complement(7826..8203)
                     /gene="rpsM"
                     /locus_tag="PKY09_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNB12272.1"
                     /translation="MARIAGIDLPRNKRGEIGLTYIFGIGRSTAQQILDECGISYDKK
                     VDQWNDDELNKIRSFINDNLKIEGALRSETQLNIKRLMDIGSYRGVRHRLGLPVRGQR
                     TKNNCRTRKGKRKTVANKKKVTK"
     gene            complement(8250..8366)
                     /gene="ykgO"
                     /locus_tag="PKY09_05140"
     CDS             complement(8250..8366)
                     /gene="ykgO"
                     /locus_tag="PKY09_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNB12273.1"
                     /translation="MKVKPSLKKRSVDCKIVRRNGKLYVINKKNPRFKQRQG"
     gene            complement(8398..8616)
                     /gene="infA"
                     /locus_tag="PKY09_05145"
     CDS             complement(8398..8616)
                     /gene="infA"
                     /locus_tag="PKY09_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNB12274.1"
                     /translation="MAKQTSIEQDGTITEALSNAMFRVELENGHEIIAHISGKMRMNY
                     IKILPGDKVKIEMSPYDLTKGRITYRYK"
     gene            complement(8630..9454)
                     /gene="map"
                     /locus_tag="PKY09_05150"
     CDS             complement(8630..9454)
                     /gene="map"
                     /locus_tag="PKY09_05150"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNB12275.1"
                     /translation="MVQIEYRSKEEIDLIRVSSLLVGKTIAEVAKHLRDGITTGELDK
                     IAEDFIRSHEAVPAFKGYHGFSGSLCISVNAEVVHGIPGARVLHLGDIVSLDCGVVKN
                     GYYGDSAYTFAIGEVDQQAIKLLQVTRECLYLGIEKAVAGNRMGDVSSNIQKHAEANG
                     FGVVRELVGHGIGKKLHEKPEVPNYGKAGQGLLLKPGLVIAIEPMINMGTKNVKQLND
                     GWTIVTADQKPAAHFEHTIAVDNVKAEILSSFEEIEKSEKENLNLYKIDYKQHIIV"
     gene            complement(9463..10824)
                     /gene="secY"
                     /locus_tag="PKY09_05155"
     CDS             complement(9463..10824)
                     /gene="secY"
                     /locus_tag="PKY09_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNB12276.1"
                     /translation="MKELIQTLRNIFKIEELRTKLLNTFFYIAIYRLASHIVLPGVDP
                     QQLGNLKNQASQGIFGLLDMFAGGAFSQASIVALGIMPYISASIVVQILGIAVPYFQK
                     MQKEGESGRNRMNQITRWLTVLVVAGQAPGYLVNLRAQAQSAIVAYANPDAFSPTMWW
                     ITSIIILIAGTLFVMWLGERIQEKGIGNGISLLIMVGIIARLPFALRDEFLARLNQTG
                     GGLIMFLVELVALFAVVVISILLVQGTRKIPVQFAKKIIGNRQYGGVRQYIPLKVNAA
                     GVMPIIFAQAIMFIPATVAQFFPESSSMQGIAHSLTDFHSVWYNVTFAILIILFTYFY
                     TAITVNPNQMADDMKKNGGFIPGIKPGRQTASFIDDVMSKITLPGSIFLAIVAILPAF
                     AKILGTSTNFAQFYGGTSLLILVGVILDTLQQVESHLLMRHYDGLMKSGRIKGRTSIN
                     APV"
     gene            complement(10824..11276)
                     /gene="rplO"
                     /locus_tag="PKY09_05160"
     CDS             complement(10824..11276)
                     /gene="rplO"
                     /locus_tag="PKY09_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNB12277.1"
                     /translation="MNLSNLKPAKGSTKKSKRIGRGEGSGHGGTSTRGHKGAQSRSGY
                     SRKFGFEGGQMPLQRRVPKSGFKNFFRVEYTGINLDVLQKLNEKFGLTEIDHSILCEK
                     GLISKRELVKILGRGELKVKMNVKAHAFSDTAKNAIEAAGGTTTIQEK"
     gene            complement(11288..11470)
                     /gene="rpmD"
                     /locus_tag="PKY09_05165"
     CDS             complement(11288..11470)
                     /gene="rpmD"
                     /locus_tag="PKY09_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNB12278.1"
                     /translation="MGKVIIKQVKSGIDRNKNQKLTLKALGLRKVYHSVEKENTDQIK
                     GMIEKVRHLVEVTEIK"
     gene            complement(11481..11996)
                     /gene="rpsE"
                     /locus_tag="PKY09_05170"
     CDS             complement(11481..11996)
                     /gene="rpsE"
                     /locus_tag="PKY09_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNB12279.1"
                     /translation="MATANKRVKPGEIELKDRLVAVNRVTKVTKGGRTFSFAAIVVVG
                     DEKGVVGYGLGKAKEVTDAITKAIDDAKKNLVKVTVHKTTVPHEQDAAFGGAKVFLKP
                     AAPGTGVIAGGAMRAVLESVGISDVLAKSKGSSNPHNVVKATITALAMMRDAVTVANQ
                     RGVDLNKVFNG"
     gene            complement(12009..12359)
                     /gene="rplR"
                     /locus_tag="PKY09_05175"
     CDS             complement(12009..12359)
                     /gene="rplR"
                     /locus_tag="PKY09_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNB12280.1"
                     /translation="MKNSKDSRRLKIRKRIRGKIKGTNEQPRLSVFRSNKQIYAQIID
                     DVAGQTLAAASSAENGAAKGTKVEISTSVGKMIAERAAAKGITKVTFDRGGYLFHGRV
                     KALADGAREGGLKF"
     gene            complement(12373..12927)
                     /gene="rplF"
                     /locus_tag="PKY09_05180"
     CDS             complement(12373..12927)
                     /gene="rplF"
                     /locus_tag="PKY09_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNB12281.1"
                     /translation="MSRIGKLPVSVPSGVQVNVQGNTVSVKGPKGTLSQTIADGITLE
                     MEENKVVVKRSTEQKRHKSLHGLYRSLLANMIKGVSQGYKIEQELVGVGYRASNQGQM
                     LDLVLGHSHHVVFELPTEVKVSTKTERGSNPTIILESHDKQLIGQVAAKIRSLRKPEP
                     YKGKGIKFVGEVLRRKAGKSAAKK"
     gene            complement(12939..13337)
                     /gene="rpsH"
                     /locus_tag="PKY09_05185"
     CDS             complement(12939..13337)
                     /gene="rpsH"
                     /locus_tag="PKY09_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNB12282.1"
                     /translation="MTDPISDYLTRLRNAVKANHRIVEVPASNTKKEITKILFDKGYI
                     ANYKFEETDNKQGNIKVALKYNPVTKMPAITRLKRVSRPGLRKYVSHDRLPRIINGLG
                     VAIISTSKGIMTDKEARQLHVGGEVLCYIY"
     gene            complement(13368..13637)
                     /gene="rpsN"
                     /locus_tag="PKY09_05190"
     CDS             complement(13368..13637)
                     /gene="rpsN"
                     /locus_tag="PKY09_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNB12283.1"
                     /translation="MAKESIKARSRKREQLVEKYAAKRAALKEAGDYQALALLPRNSS
                     PVRLHNRCMITGRPRGYMRQFGISRVLFRKMALEGKIAGVTKASW"
     gene            complement(13654..14211)
                     /gene="rplE"
                     /locus_tag="PKY09_05195"
     CDS             complement(13654..14211)
                     /gene="rplE"
                     /locus_tag="PKY09_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNB12284.1"
                     /translation="MSYTPRLKGKYKKEIIPALKNHFQYKSIMQVPKLSKICLSQGIG
                     DAVSDKKIVDACVAEMTAIAGQKAVATYAKKDISNFKVRKGVPIGVRVTLRDEQMYEF
                     LDRLVSVSLPRIRDFRGINNKGFDGKGNYTLGITEHIIFPEINIDKVSKIRGMDITFV
                     TTAKTDNEAMQLLKEFGLPFRTGSN"
     gene            complement(14211..14528)
                     /gene="rplX"
                     /locus_tag="PKY09_05200"
     CDS             complement(14211..14528)
                     /gene="rplX"
                     /locus_tag="PKY09_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNB12285.1"
                     /translation="MSKLKIKKGDIVKVIAGESKGKQGRVLEVFPETNRASVENVNIV
                     SKHTRPNAQNTQGGIVKKEAPVNVSNLMLIDPKSGKPTRVGRKTDEGKLVRYAKKSGE
                     VIK"
     gene            complement(14546..14914)
                     /gene="rplN"
                     /locus_tag="PKY09_05205"
     CDS             complement(14546..14914)
                     /gene="rplN"
                     /locus_tag="PKY09_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNB12286.1"
                     /translation="MIQSESRLTVADNSGAKEVLCIRVLGGTRKRYASLGDKIVVTVK
                     NALPAGNIKKGAVSKAVVVRVSKEVRRNDGSYIRFDDNAVVLLNQADELRGTRIFGPV
                     ARELREKQFMKIVSLAPEVI"
     gene            complement(14966..15226)
                     /gene="rpsQ"
                     /locus_tag="PKY09_05210"
     CDS             complement(14966..15226)
                     /gene="rpsQ"
                     /locus_tag="PKY09_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNB12287.1"
                     /translation="MSTERALRKTKIGVVTSIKMQKTVVVAEEKKFKHPMYGKFINKT
                     KKFHVHDEANACGSGDRVLIMETRPISKTKRWRLVEILEKAK"
     gene            complement(15235..15435)
                     /gene="rpmC"
                     /locus_tag="PKY09_05215"
     CDS             complement(15235..15435)
                     /gene="rpmC"
                     /locus_tag="PKY09_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNB12288.1"
                     /translation="MKQSIVRDLTTEEVKARIKEEADNLQKHKMQHAVSPMDNPMKIR
                     STRKLIARLKTELNKRMSQTSK"
     gene            complement(15455..15877)
                     /gene="rplP"
                     /locus_tag="PKY09_05220"
     CDS             complement(15455..15877)
                     /gene="rplP"
                     /locus_tag="PKY09_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNB12289.1"
                     /translation="MLQPKKTKFRKQQKMRNRGNATRGSQIAFGSFGIKSLENAQLTS
                     RQIEAARVALTRFMKREGQTWIRIFPDKPVTRKPAEVRMGKGKGAPEYWIAQVKPGTM
                     LFEADGVSVETAKEAMRLAAQKLPVITKFVIRRDYSAA"
     gene            complement(15909..16673)
                     /gene="rpsC"
                     /locus_tag="PKY09_05225"
     CDS             complement(15909..16673)
                     /gene="rpsC"
                     /locus_tag="PKY09_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNB12290.1"
                     /translation="MGQKAHPIGNRLGIIRGWDSNWYGGKNYSDRLVEDEKIRNYLHA
                     RLAKGGVARIIIERTLKLITATIHTARPGIVIGKGGSEVDKLKEEIKKITKKEVQINI
                     FEIKRPELDAQLVADGVAKQIEARISYRRAIKMAIASSMRMGAEGIKIMCAGRIGGAE
                     MARTEQYKDGRIPLHTFRADIDFAIADAQTSYGKIGVKVWICKGEVFGKRDLSPNVGM
                     NSTPKQNAGGERRQERERGGNERGGRRGGRPRGEKK"
     gene            complement(16674..17114)
                     /gene="rplV"
                     /locus_tag="PKY09_05230"
     CDS             complement(16674..17114)
                     /gene="rplV"
                     /locus_tag="PKY09_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNB12291.1"
                     /translation="MGARKKIAADARKEEMKTTYIARLNNCPTSPRKMRLVADMIRGK
                     KVDLALGLLKFSKKEAAGNMYKLLLSAINNWQAKNEGVRIEDQPLVVSKVYVDSARQL
                     KRLRPAPQGRGYRIRKRSNHVTLFVDTVNKKVENTQQVTENTEA"
     gene            complement(17114..17386)
                     /gene="rpsS"
                     /locus_tag="PKY09_05235"
     CDS             complement(17114..17386)
                     /gene="rpsS"
                     /locus_tag="PKY09_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNB12292.1"
                     /translation="MSRSLKKGPFIDPKLEGKVASMSEGKKKTVIKTWSRRSLISPDF
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFAPTRTFRGHAGNKDKK"
     gene            complement(17433..18257)
                     /gene="rplB"
                     /locus_tag="PKY09_05240"
     CDS             complement(17433..18257)
                     /gene="rplB"
                     /locus_tag="PKY09_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNB12293.1"
                     /translation="MAIKKFNPITPGQRFLIAPDYSNITTNEPEKALTERINRKGGRN
                     NSGKMTMRYIGGGHRKLYRIIDFKRNKDGVPGKVATIEYDPNRTARIALVHYADGEKR
                     YILAPAGLTVGQAILSGDKATPEVGNAMMLKDMPLGTIVHNIELRPGQGGKMARSAGS
                     YAQLTARDGKYAILKMPSGETRMVLSTCVATIGSVSNSDHNKESAGKAGRNRWKGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGKKTRKLKNQSNKHIIERRKK"
     gene            complement(18268..18558)
                     /gene="rplW"
                     /locus_tag="PKY09_05245"
     CDS             complement(18268..18558)
                     /gene="rplW"
                     /locus_tag="PKY09_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNB12294.1"
                     /translation="MSILKKPVITEKMTQKSEKLGQYAFIVDKNANKIEIKAAIEKLY
                     NVNVVSVNTVNNAVKSKTRFTKAGILQGKKGGQKKAYVTVAKGETIDFYASI"
     gene            complement(18555..19184)
                     /gene="rplD"
                     /locus_tag="PKY09_05250"
     CDS             complement(18555..19184)
                     /gene="rplD"
                     /locus_tag="PKY09_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNB12295.1"
                     /translation="MELEIKQRNGQATGRKVTLNSEIFAATPNDHAIWLDVKSIMANA
                     RQGTHKSKQRNEVSGTTKKLKRQKGTGGARAGSMKSPLFVGGGRVFGPQPRDYSFKLN
                     KKVKRLARISALSYKAKDNNITIVENVTMEAPKTSEVVNILKQLELANKKVLVVTGDT
                     NKNLFLSSRNLQRSKVLSASEINTYDILNAQNLVLEEGALPVIEKILNK"
     gene            complement(19190..19807)
                     /gene="rplC"
                     /locus_tag="PKY09_05255"
     CDS             complement(19190..19807)
                     /gene="rplC"
                     /locus_tag="PKY09_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNB12296.1"
                     /translation="MSGIIGKKIGMTSMFNAEGKNIACTVIEAGPCVVTQVKSVATDG
                     YDAVQLAFDDKKEKHTTKALQGHFKKSNSSFKRKVVEFKGFTTEVKLGDVIKVDICPE
                     GEFCDVVGTSKGKGFQGVIKRHNFAGVGGATHGQHNRLRAPGSMGASSFPSRVLPGKR
                     LTGHMGDEQVKIQNLQILKVMPEHNILIIKGAVPGAKGSYIKVEF"
     gene            complement(19831..20136)
                     /gene="rpsJ"
                     /locus_tag="PKY09_05260"
     CDS             complement(19831..20136)
                     /gene="rpsJ"
                     /locus_tag="PKY09_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNB12297.1"
                     /translation="MNQRIRIKLKSYDYNLVDKSAEKIVKTVKTTGAVISGPIPLPTE
                     KKIYTVLRSPHVNKKAREQFQLCSYKRLLDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            complement(20172..22295)
                     /gene="fusA"
                     /locus_tag="PKY09_05265"
     CDS             complement(20172..22295)
                     /gene="fusA"
                     /locus_tag="PKY09_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB12298.1"
                     /translation="MANDLRFTRNIGIAAHIDAGKTTTTERILYYTGVNHKLGEVHDG
                     AATMDWMVQEQERGITITSAATTCFWNYRNNKYKVNIIDTPGHVDFTVEVNRSLRILD
                     GLVFLFSAVDGVEPQSETNWRLANNYNVTRLCFVNKMDRAGADFLNVVKQVREMLGGN
                     AVPLQLPIGAEENFKGVVDLINFKGIVWNEHDQGMTFTEVPIPAEILEEAKSLREKLL
                     ESIAEFDDKLMEKYFDDPNSITEREILDALRKSCIAGKVVPMVCGSSFKNKGVQTMLD
                     LVMELLPSPIDKDNITGTNPDNGEPVSRKPDVKEPFTALAFKIATDPFVGRLAYFRAY
                     SGRLESGSYVLNSRSGNKERISRIFQMHANKQNQIPFIEAGDIGAAVGFKDIKTGDTL
                     CAENAPIVLEAMQFPEAVIGLAIEPKTQVDTEKLGTALNKLAEEDPTFRVKTDEETGQ
                     TIISGMGELHLEIIVDRLKREFKVECNQGAPEVSYKEAITGSVSHREVYKKQTGGRGK
                     FADIQFTLSPVDEDFDPVANKSNLQFVNQITGGNIPKEFIPSIEKGFKAAMVNGVLAG
                     YPLSSLKVVLTDGSFHAVDSDALSFEIAAKLAYKEACAKAKPILLEPIMKVEVVTPDE
                     YMGDVMGDLNRRRGQMEGMDTRAGAQVIKAKVPLSEMFGYVTTLRTITSGRASSSMEF
                     SHYTECPRAIAEEVIAKVKGKLEKA"
     gene            complement(22317..22784)
                     /gene="rpsG"
                     /locus_tag="PKY09_05270"
     CDS             complement(22317..22784)
                     /gene="rpsG"
                     /locus_tag="PKY09_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNB12299.1"
                     /translation="MRKSKPKKRILLPDPRYNDQQVTRFVNGMMESGKKGTAYQIFYD
                     ALEKVTEKTQEDGLEQWRKALNNVMPLVEVRSRRVGGATFQIPSEVRPTRRTALGIKW
                     LVGYARSRNEKSMADRLAGEIIAAAKGEGAAVKKKDDTHRMAEANKAFSHFKV"
     gene            complement(22815..23210)
                     /gene="rpsL"
                     /locus_tag="PKY09_05275"
     CDS             complement(22815..23210)
                     /gene="rpsL"
                     /locus_tag="PKY09_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNB12300.1"
                     /translation="MPTIQQLIRIGRSKIEDKSKSPALTSCPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNKFEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGAL
                     DTSGVEGRNQRRSKYGTKKPKAGAPAAKK"
     gene            complement(23940..24395)
                     /locus_tag="PKY09_05280"
     CDS             complement(23940..24395)
                     /locus_tag="PKY09_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12301.1"
                     /translation="MAKIENKDFNVEMQIRAIELLNGSLQLPAAPNTAITTFNFNISI
                     ESKADAPNKLVFVIVHVEIMNDDHSIVLGALSVSCIFEIVNFDEVIKIEDNGKLDIPQ
                     QLIETLNIISISTTRGVMFSTFKGTFLHGAVLPIIDPKQFQIGNQKVEK"
     gene            complement(24397..24855)
                     /locus_tag="PKY09_05285"
     CDS             complement(24397..24855)
                     /locus_tag="PKY09_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB12302.1"
                     /translation="MNKTTKARKYNSSKLQKLLDEVTPLEMEQTKVKMQLAARIEDFM
                     RAKGWNKSQFAEKVVKNPSEITKWFSGTQNFTTDVLTEIAFALGVDLTALFGKKQVQV
                     IYRKEIVVKTVVTQPTIVMTTPMSQGIDVYEYCLLASQGNNSQFTKYYQA"
     gene            complement(24848..25342)
                     /locus_tag="PKY09_05290"
     CDS             complement(24848..25342)
                     /locus_tag="PKY09_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12303.1"
                     /translation="MNYEIVELQPYSGSEAKVYSLIPMGEDETLFEKFVDEYKVEFKN
                     EVKDIIKTIYQIGHTTGARSSFFKQHEGKYGDFVCALFDVPEKNLRVYCIRFGMVAVI
                     LGGGGEKAEGVRAWQDDEKLSKEANQMIEYAKDILQRMDDGDLYWSKDRTELQGNLKN
                     YDNE"
     gene            25830..26051
                     /locus_tag="PKY09_05295"
     CDS             25830..26051
                     /locus_tag="PKY09_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HNB12304.1"
                     /translation="MYNTIEIDRDNLTIMGIKFSDIKTLESTANALGSNMFEGFKPTP
                     KGIEIIRDYVTGKISLAELVAFAKQKAYV"
     gene            26044..26184
                     /locus_tag="PKY09_05300"
     CDS             26044..26184
                     /locus_tag="PKY09_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12305.1"
                     /translation="MSNSYKYIDPEFSYTDPKTGLLKNLQDITDPDILLFVQSGVVTK
                     RL"
     gene            26218..26652
                     /locus_tag="PKY09_05305"
     CDS             26218..26652
                     /locus_tag="PKY09_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNB12306.1"
                     /translation="MDSLFEIHRHLFQDIYAWAGKRRIVEISKDGKQFFPTSQFDNAF
                     RHIDQLIADFKKIPGENKNQLADKLAEILDNLNYLHPFREGNGRTQREFLRLLALEKG
                     LTLNLNPPDNKSVYERYMKGTIESEVKTLAELIFELIDENEK"
     gene            26933..27781
                     /locus_tag="PKY09_05310"
     CDS             26933..27781
                     /locus_tag="PKY09_05310"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HNB12307.1"
                     /translation="MTTFSKKTDMNFVDWGEIDMPKAVYKYRTWSDKYHKTILTNLQV
                     FMSPPLGFEDPHDCKNLIRYDLLTSNEIFWKYHQESLERNPRFTPEQHQGFAQYWFER
                     SPLHDKDFIKQQQEKDFIEEDKRLGILSLTTNPINNSMWEKYSENHCGFCVGFHSEIM
                     FKFLGGGGAVNYCDELPIIKPTPWDDYNMQHLKQVYYKLRKWSFEEEYRTQKFSYEPL
                     TVQNRTITLPSEAYKEIIFGAKMNEKDMQEIISICKYNLSHIEFYQAVLNNSNDNVSI
                     EKYNSR"
     gene            28112..30601
                     /locus_tag="PKY09_05315"
     CDS             28112..30601
                     /locus_tag="PKY09_05315"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB12308.1"
                     /translation="MVRKIHFIFLLTLWCSVLSAQSFNPSEIRLNLEKLNTVGTVLYI
                     AAHPDDENTRLLTYLSKEKHLRTCYLSLNRGEGGQNLIGKEQGAALGIIRTNELLQAR
                     KIDGAEQYFTRAIDFGYSKSPEETFTFWNHDSVLADVVYIIRLLQPDVIITRFPTTGE
                     GGHGHHTASAILAGEAFKLAADTKYLPEQLKSLKPWQAKTLYWNTFNFGNNNTQRDDQ
                     LKTDVGFFNTWWGKGCGEIAALSRSQHKSQGFGVEIIRGSMLEYLKNIDGDTSCTTLF
                     CKNNFSWTRVKGAETIKSLTETALVKFDMKNPSAILPDLLKLYDELKKLQDNHWKKQK
                     IKETEQLILACAGLWFDATSNQPYVTHGDSINIKLSSIVYSQTPTTIESVSYLPFSDT
                     SMSTKLDYNKIWSLEKSFVVPKNIPYSFHYWLKNIPESGRYVLDSQNIIEKPVNDAVF
                     SVSVGVKISDHTLTFNVPVRYKWIDPVEGEKYRTTSVVPPIAVNFNENVCLLINNKPG
                     KLKLTVRSLSKSFTGKIKLNVPSGYIANPSEFAVTELLKNERLIFNTTIQQDVKADVD
                     QINANQHLQVSILPTNSEAYSLQEINYEHIPYQYYFKPSEVKIVPLELQTSSNRIGYI
                     EGAGDEVMQCLQNAGYNISVLNEENLRNDNLSNYSVIIAGIRAYNTNDWLIGYKNKLM
                     DFVSSGGTYIVQYNTNNNLGKLGNNIGPYPFKISRKRVTDEFAEMRFTEPANKTLNTP
                     NKITANDFRGWVQERGIYFAEEIDPKYQTPLSCNDKGESPLNGSLIIAQSGKGHFVYT
                     GLAFFRQLPAGVPGAYRLFSNLIALPSGQEK"
     gene            30650..31324
                     /gene="trmB"
                     /locus_tag="PKY09_05320"
     CDS             30650..31324
                     /gene="trmB"
                     /locus_tag="PKY09_05320"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002677913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNB12309.1"
                     /translation="MGKDKLKRFAEMQTFERVIQPEINYHSPDFELKGKWNETIFQKP
                     QPIVLEIGCGKGEYTVALAQRYQQKNFIGIDIKGARIWRGAKTINEEKIMNAAFLRIR
                     METIEKFFAANEVSEVWITFPDPQPKESKENKRLTSPLFLKRYSTFLIPNGIVHLKTD
                     NKDLYEFTLQTVNNAGHIILNKTTDLYKESKMPVDMDIQTTYEKKYLAAKQPIFYLSF
                     QLNEKR"
     gene            complement(31334..31669)
                     /locus_tag="PKY09_05325"
     CDS             complement(31334..31669)
                     /locus_tag="PKY09_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB12310.1"
                     /translation="MSKSIDEFNSYRAKMNKRILESDNLVLKRLYNLDANTYMDGALP
                     VQTKEMLGLVASMVLRCDDCIKYHLIQCKQNGVTTEQIYEVFAVANIVGGTIVIPHTR
                     RAAEFWDEI"
     gene            complement(31681..32466)
                     /gene="tatC"
                     /locus_tag="PKY09_05330"
     CDS             complement(31681..32466)
                     /gene="tatC"
                     /locus_tag="PKY09_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNB12311.1"
                     /translation="MNFLDHLEALRWHLVRATLAIFVLAVVAFIYKDILFDKIIFGPK
                     NPHFITYRLLCMLSEKWSIDLCITDIPFRLISTTMAGQFTTHMVAAGIAGLILGFPYL
                     LWEIWRFVKPALSAKEKKYTRGIVFSASFLFFCGVAFGYFILAPLSINFLGSYKVSEE
                     VQNAITLDSYISIVSIMTLACGAVFELPILVFFLTKIGIISPQFMRRYRKHAFIVNLV
                     VAALITPSPDVTSQMLVAIPLFLLYEFSIFVSVVVARQERKNE"
     gene            complement(32518..33435)
                     /locus_tag="PKY09_05335"
     CDS             complement(32518..33435)
                     /locus_tag="PKY09_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNB12312.1"
                     /translation="MESAAVSGLQQYIDHDFFETVKLIYLSKGRVVCTGIGKSAIIAQ
                     KITATLNSTGTPAVFMHAADAVHGDLGVIQKDDVVICISKSGNTPEIKVLAPLLRLAG
                     NKLIAMVGDTSSYLAQQAHYILNTTVSREACPHNLAPTTSTTAQLAMGDALAVCLLEN
                     RGFSSKDFARYHPGGALGKRLYLKVGDVYKNNASPQVKPTDDLKKVIVEISGSRLGAT
                     AVVEKGKLKGIITDGDLRRMLHRSGDTLATKAKDIMTSKPKTVSPDTMAADAFVLMKS
                     NNITQLVVADKGKYLGFVHLHDLLKEGIF"
     gene            33592..>34614
                     /locus_tag="PKY09_05340"
     CDS             33592..>34614
                     /locus_tag="PKY09_05340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNB12313.1"
                     /translation="MLADTKKKKSVKDKNEKTDTKTKVKVKEKVVEKKTKGTEKAKPV
                     VKTIVAASNKQKPTDARLKDALFKYFGFDTFKGEQEEIIQTVLRGENCFVIMPTGGGK
                     SMCYQLPALMMGGTAIVVSPLIALMKNQVDALRGFNTDEAVAHFLNSSLNKTEIAQVK
                     KDIKSGKTKLLYVAPESLTKEENVAFFKEIKVSFLAIDEAHCISEWGHDFRPEYRRLK
                     PIIEALGNIPVIALTATATEKVSLDIQKNLGMMESRLFKASFNRPNLYYEVRPKVEVL
                     KEIIKFVKNHSGKSGIIYCLSRKKVEEVASTLNVNGIKALPYHAGLDAPVRAETQDKF
                     LMEKIDV"
BASE COUNT        10185 a         7060 c         6073 g        11296 t
ORIGIN      
        1 tgatgaccta aagcaaaggt tgcctatgtt acttggtgga actgatgagg cagcggcatt
       61 ttatcgtttc atgtttttct cattattctc ctatgtttct catcgcatcc ctgaaattgc
      121 agatgaaatt tacaagattg atgatgccat gtgcgctggc ttcggctggg aaattggccc
      181 cttcgaaaca tgggatattc ttggcgtgca atccgtagta acacaaatgc agaaagaagg
      241 ttacagcgtt gccccttggg tgacagaaat gattagtaat ggaataaatt ctttctataa
      301 tattgataat ggcaaaaaat catatttcga tttacagact aaaaaattaa atccactccc
      361 tggcgaagga ggtttgatta ttcttaaaaa tacttcaagc gaaaaaattg tctggaaaaa
      421 ttccggatgc ctgttacatg atattggtga tggtgttttg aacctggaat tcaaaactaa
      481 aatgaatact attggcagtg aagttttaca gggcattaat actgccattg attttgcaga
      541 aaaagatttt aaaggattag taattggcaa cgaagcagtc aatttttctg caggtgctaa
      601 tctgggtttg gtttttatgc ttgctgttga acaggaatat gatgaacttg attatgctgt
      661 aagggcattt caaaatacaa atatgcgcct gcgttattgt aatgtgcctg ttgtagtagc
      721 tcctgcggga cttacattag gcggtggctg cgaaatgagt ttacatgccg acaaagtgca
      781 agcggccgca gaaacttata ttggtcttgt tgagttaggt gttggactaa tacccggtgg
      841 aggtggcacc aaagaattaa cgcttcgttt ttcagactcc attaaacccg gagatattga
      901 aacaaatgca ctgcgagatt tttttatgac tattgccaca gcaaaagttt caacttcggc
      961 atttgaagct tacgatttac atattcttag aaaaggattt gacaccataa cacttaacag
     1021 aaaaagactt ttgtctgatg ctaaaaatgc agtgctatca ttagctaatg ccggatatgt
     1081 gagtccacaa ccacgacatg atattcgtgt tttaggaaga caaggtcttg gtgtgattca
     1141 ggccggtgcc agcgctatgc tggcaggcaa ctacatcagc caatatgatc agatgctttc
     1201 gttaaaactt ggatacatta tgtgtggcgg caatttaagt tcgccttctt tagtatctga
     1261 aaaatattta cttgatctgg aaagagaagc ctttctttct ttattaggag aaaagaaaac
     1321 acttgagcgc attcaggcaa ttttaaccac tggcaaagcg ttaagaaatt aatatttaat
     1381 catttgaagt agtatttaga aatttaatgg ttttcaacaa tttattggaa gtcagcattt
     1441 gtttttcctc tgaaaaacag aaattaaatg agttgtttat aaatgaaaaa catgtagaaa
     1501 aactctgaat ttcattcatg ttttgattaa aaatatttta ttttagccat ttgaattacc
     1561 ataccaatca taaaagatga aaatattaaa acaaccgttg ttgttgttaa tcctcttacc
     1621 ggcttttgct tttgctcaac gcgaagaaaa accacgctat gcacccggaa gagaaaaaca
     1681 tcgcaacaag acagatgagc taggacagcg gcaaggtgtt tggaaatatt ataatgaagc
     1741 cggtgaatta aatcaggaaa ttgaatattt agatgatgta agaaatgggg tagcaaaaaa
     1801 atattatcca tatggaaaga tattagagga gattgaatat gctaatggta ttaaggatgg
     1861 agcttatacc cgatattatt ataacggaca ggctaagata gaaggtgaat atctgaatgg
     1921 taaaaaaagt ggtcagtgga ctacctattt cagcgatgga gaaattagaa attcaggtcc
     1981 gtataaatta aatttgaaaa atggtctttg gaaattttac gacaggaaag gtaaacttct
     2041 ccgacaaaca gaatataaga tgggtgaaga cgtaaaagca ctggcagctg ccaaggaagc
     2101 tgaagaaaaa gctaaaaagg cagatgcagc taaaaaagcc gctgaggcta aaaagaaatt
     2161 acctcccgga gctaaattaa aaacgcctcc acctgcaaca cctgaaaaga aataaatttc
     2221 ttttatataa cctgtgacca gcaaaaatat atttaatagc aagtacaaat ggatgcatac
     2281 atcgtagccg gatacagaac tgctgttggc aaagcaccaa gaggtaaatt taaaaataca
     2341 agaccggatg atcttgctgt tactgttata aaacatctca ttcaatctgt tccgaattta
     2401 gatacagagc taattgatga tgttattgcc ggaaatgcta caccggaagc agaacaaggc
     2461 ctaaatatcg gaagaatgat ttctttgatg ggacttaata ctgttaaagt tccggggatg
     2521 actattaacc gttattgttc atcaggatta gaatccattg ctattgcatc agcaaaaatt
     2581 cacagcggcc ttgctgattg tattattgcc ggtggtgttg aatgtatgtc acctattcct
     2641 tttggcggat ggaggattgt accaaactat gaggttgcta ccaatcatcc tgaatggtac
     2701 tggaacatgg ggttaactgc agaagcagtt gcaaaagaat ttaaaattaa ccgtgaagac
     2761 caggatcaat tttcttttca atcacatcaa agagctatta atgcaataaa aaacaatttg
     2821 tttaaagagc agattgttcc cgttgaaatt aatgaaactt atcttgatgc caacgaaaaa
     2881 agacagacac ataaaagcat tgttgatact gacgaagggc cacgccagga tactacaata
     2941 gaaaagttga attcattaaa accggtcttt gatgctaaag gaactgttac cgcaggtaac
     3001 tcatcacaaa caagtgatgg tgcttcattt gtacttgtag tgtctgagaa aatgctcaaa
     3061 acacttaatg taaaacctat ggcacgtttt gtttcttatg cagtttgtgc aatagagcca
     3121 cgtattatgg gtgtaggacc tgtttatgct attccatctg ttctgaaacg cgcaggactc
     3181 aagcaagatg atattgaact tattgagctt aatgaggctt ttgcatcaca atctttggct
     3241 gtaatccgaa ctttagggtt aaatccggaa atcaccaatg taaatggtgg tgcaattgca
     3301 ttaggacatc cattaggttg ctccggtgct aaacttagtg ttcaacttct gaatgaaatg
     3361 aaaagaagaa aaatgaaata tggcatggtc accatgtgcg taggtacagg acaaggcgct
     3421 gcgggcattt acgaagtact gtaagtctta ttattttttt gctgttaaag caattttaaa
     3481 atccattgga atgggattac aaacactgta caaatcatca ataaattgta atccataaag
     3541 attcaaataa ggaaatacat tctctattct ttcctgtaat ttaccgtttg gaaataattt
     3601 ttcaaccaca tttctgatct tacgagtctc gtcaacctgc tttttcttaa atgcgcgcag
     3661 aagttttttt tctgaggtca tcaaacgttt ctctgctcgc ctccactctg ccttcacatg
     3721 tttatctaat gtgacatcaa ttgctttaaa gcgttgctcc atctctgcat agcttcgttg
     3781 tattgcatct ctttcgactt taaggcttag ctcttcaacg tactttcttt tcaatgcaat
     3841 attgacaact tgttcggtgg gatgaaataa atctttccat accaaagctg attcattcaa
     3901 aaaattagct tgatctgcat ttacaaccaa catgccatta cgaagaacaa ccatcggata
     3961 aaaaatattc agcttatcga agataggttt taattgcaac cagtaatgca cttctgccgg
     4021 gccaccggta tatgcaatgt ttggcaaaat tacttcctga tataacgggc gtagcacaac
     4081 atttggacta aatcgttccg gatatgcatt caactgcttc ttcaaatcat cacttgcgaa
     4141 agacaagccg gaatctgcaa cttcaaaatt ttcaccattt ctaactatcc tgttacgctc
     4201 tccatcggct aaataaaaca aattaatttc tcttgcatga atttgtaagc catacttctt
     4261 ttcaaactca gaaaggtttt taaaacaatt aaaagcagtg tttttgaaca aatcatcttc
     4321 aaacatttca attgcttgct gcttgagtct tttatcatct gcatctataa ttactaaacc
     4381 atattttgaa aataaagaat gtacaagact ccttatagca ttagcttgcg ttgcattttt
     4441 taaatatgca gtgtcaaaca tttctgctat atcctgaaaa tattcatttt tatgtaaatg
     4501 atttttcaat gtttcaacaa cagaactcat tccttccgtt gtcattctac ctacagcccc
     4561 tttttgcttt gtctgccatt caattttgga ttcatcaatg gaaacatgat tcacttctgc
     4621 aaggtcatgg tcttcgctgg ccatccaata aacaggcaca aacttataat cgggattttt
     4681 ttgtgacaga aattctgcct gcttaattac cgtgagtatt ttataaataa aatataacgg
     4741 ccctgtgaaa atattcagtt gatgtcctgt tgtgatagta aaagtatgct cctccttcaa
     4801 tgatttaatc gcagaatatg atttacttgt tttccaatct gtttgaaaaa ctgtttctga
     4861 atactgatgt tctaatactt cgcaaaggct gtcacggttg gtcctatatt gtaatcgctt
     4921 ctgtaatgct tcctttaaac ctgattcatc atattgaaat gctgcatatt ctttaaaaaa
     4981 actactctta cttaaataat caccaattaa tgtagaatat ccggcatcag taaaagatat
     5041 ataagtactt tgagctaaca taaatgcttc atcagaaaaa tcactgtaaa cataaaaata
     5101 aaaaaaggga taccgaaacc ggcatcccca ttaaattaat ttttctgttg aatttactct
     5161 tctgttgtac ctgttgtttc gcttcctgca gcatcagctt tagcctttgg cttacggcct
     5221 ctacgagtag atttagcttt cttagcagta cctttttctt ttgatgaagg catcaactca
     5281 ttgaaatcaa ctaattccat catgcaagtt tcagcggcat caccgggtct gaatccggtt
     5341 ttaaggatac gggtatatcc acccggacgc tcattaacct taggagcaac ctctctgaaa
     5401 agctcactaa cagcttcttt attctgcaga taactaaaaa ctgttctgcg tgaatgtgtg
     5461 ctgtcagatt ttgactttgt aattaatggt tcaacatatt ttctcagtgc cttagctttg
     5521 gcaacagttg tgtttattct cttatgcaga atcaatgatg aagccaggtt agagagtaat
     5581 gcttctctgt ggctatgtgt tctgcctaaa tgatttatct tttttccgtg acgcattttt
     5641 cgtaatcttt aaactattca attattcttt atcaagcata tacttctgta tgttcatacc
     5701 gaaggtaagg tttttactgc gaaccaattc ctccaactca gtcagcgatt tttttccgaa
     5761 gttacggaac ttcaacaggt ctgtttttga aaaagaaacc aaatcaccca aagtctcaac
     5821 atctgctgcc tttaggcagt tcaatgctct tactgaaaga tccatatcta ccaattttga
     5881 tttaagcaat tgacgcatat gaagtgtgtt ctcatcaaac tcttcaaccg gagttttaga
     5941 ctttgtatcg agcgtaattt tctcatcgct aaacagcatg aagtgatgaa tcagaatttc
     6001 tgcagcttcg cgtaatgctt ctttaggatg aatacttcca tcggttgtga tttcaagtac
     6061 caatttttcg taatcggttt tctgctccac acgatagttc tccactgcat attttacatt
     6121 ctttattgga gtgtagatag aatctattgc aacagttcct aatggatggc tgtttgattt
     6181 gttttcttct gcaggaacat atccacgtcc tttgtcaatg gtcatatcaa taaccaattt
     6241 tacagaagga tccatctggc aaataaccaa ctcaggattg agtacctgaa aagcagcagt
     6301 atgtttagca atatcaccgg cagtaaattc tgtcttacca ttaacaacaa cctgaacttt
     6361 ttcagagtct gtgccttcaa tttgcttttt aaaacgaacc tgcttgaggt taaggataat
     6421 ttcagttaca tcttcaacaa ctccttttat tgttgaaaat tcatgatcaa ctccactgat
     6481 tttaatattg gtgattgcat gtccttcaag cgaagaaagc aatattctac gtagagaatt
     6541 accaacagta ataccataac ccggctccaa agggcggaac tcaaactgac caaaagtttc
     6601 ggtagaatga ttcataatca ctttgtcggg acgttgaaat gcaagtatgg ccattttata
     6661 ttttgtgttt tatttgttta attatttact gagttttatt gttcagggtt agcaagaatt
     6721 atttagagta caattccacg ataagttgtt ccttaatatt ctcaggaatt tcttcgcgtg
     6781 caggtgtagt cacaaattta ccttgcatag ctgtggtatc ccactctaac caaccatatt
     6841 tattagaacg agatgcaagt gaatctgtaa ttgcttcgaa agactttgag cgctcacgaa
     6901 cgccaacaac atcacccgga cgtaagatgt atgatgaaat attgtttacc ttaccattta
     6961 ctgtaatatg gcaatgtcca actaactgac gtgctgctgc acgtgttggt gcaattccca
     7021 aacgaaaaac aacattatca agtctggatt caataagctt caacaagttt tcacctgtaa
     7081 taccttcttt acgaagtgca atactgtaga aacgagcaaa ctgacgctct aacattccgt
     7141 aagtatattt caccttttgc ttttcagcta actgaattgc atattcactt tgcttagcgc
     7201 gctttttagc agcaccatgc attcccggag gataacttct tttttcgaaa tatctgtcag
     7261 gtccgaatat gggctcgcga aacttacgtg ctatttttgt ttttggtcca atgtaacgtg
     7321 ccattgtatt attctaatat tttaatttta aactaacttg tgataataaa ttatatcctt
     7381 cttcttttag gagggcgaca tccgttatgt ggtattgggg tgatgtccag aatttcgctt
     7441 acctcaactc ctgaagcgtt aagcgttcgg attgcagatt cgcgacctgc tccgggtcct
     7501 ctaacaaata cttttacctt tctaagacct aagtcgtacg ctgtttttgc gcaatcagct
     7561 gctgccagct gtgctgcata aggtgtgttt ttcttagatc ctttgaagcc cattttgcct
     7621 gcacttgccc aagaaatcac ttggccttgc aaattcgtca ggctgataat aatgttattg
     7681 aaagttgcat tgatatgcgc ctcaccggtc gcctcaactt ttaccacctt cttttttgct
     7741 gtttttgcag ctgctccttg ttgttgtttt gccattattg tttatttatt gttattcccc
     7801 ttaaaaaggg aacattttat tccaattact tagttacttt tttcttgtta gcaactgttt
     7861 tacgcttacc cttacgtgtg cggcagttgt ttttggttct ttgtccgcgc acaggcaatc
     7921 ccaaacgatg acgaacacca cggtagctac cgatatccat cagacgttta atattcaact
     7981 gtgtttctga acgtaaagca ccttctattt tcagattatc gttaatgaat gaacggattt
     8041 tattcagttc atcatcattc cactgatcta cttttttatc gtacgaaatt ccgcactcat
     8101 caagaatctg ttgagctgtt gaacgtccta taccaaaaat ataagtaagg cctatttcac
     8161 ctcttttgtt tcttggtaaa tctatacctg ctattcgagc cattttatat taactatttt
     8221 ctttgttaca attaattttt aagatgtaat tatccctgac gctgcttaaa gcgtggattc
     8281 tttttattaa ttacatacaa tttaccgttg cgtctaacga ttttacaatc aacgcttctt
     8341 ttctttaaac ttggtttaac tttcatcttc ttaatttatt ctaatgcgtt atctcaatta
     8401 tttataacga taagttattc ttccttttgt cagatcataa ggtgacattt caatctttac
     8461 tttatcaccg ggtagaattt taatgtagtt cattctcatc tttcctgaaa tgtgggcaat
     8521 tatttcatgt ccgttttcca gttccaccct gaacatagca ttgctcaacg cttctgtaat
     8581 cgttccgtcc tgctctattg atgtctgctt tgccattaat ctaatcttac tatactataa
     8641 tatgttgctt ataatctatt ttatataaat ttaaattctc tttttcactc ttttctatct
     8701 cttcaaatga agacagaatc tctgccttta cattatccac tgcaattgta tgttcaaaat
     8761 gtgcggcagg cttttgatct gctgttacaa ttgtccatcc atcattgagt tgctttacat
     8821 tctttgttcc catgtttatc ataggttcaa ttgctataac caacccgggt ttcaacaaca
     8881 atccttgccc tgccttacca taatttggta cttcgggctt ctcatgaagc ttcttcccta
     8941 tgccatggcc taccaattca cgaacaacac caaatccatt tgcctctgca tgtttttgga
     9001 tattgctgct tacatcaccc atcctgttac cggcaactgc cttctctatt ccaagataaa
     9061 ggcattcgcg tgtgacctgc agtagtttta ttgcctgctg atctacttca ccgatagcga
     9121 aggtgtaggc cgaatcacca taatatccgt ttttaactac cccacaatca agtgacacaa
     9181 tatctccaag atgcaacact cttgctcccg ggattccgtg aaccacttcg gcattaaccg
     9241 atatgcataa cgaaccgctg aatccatgat agcctttaaa agcaggcaca gcttcatgtg
     9301 agcggataaa atcttctgca atcttatcca actctccggt cgttattcca tctcgcaggt
     9361 gtttggcaac ttctgcaatg gtttttccaa caagtaaaga actaactcta attaaatcaa
     9421 tctcctcttt ggagcgatat tcaatctgca caattttata ttttaaacag gtgcattaat
     9481 ggatgtgcgt cctttaatac gtcctgattt cattaatcca tcgtaatgtc tcatcaacaa
     9541 atgactttct acctgttgta aggtatccag aattacacca acaagaatca acaatgatgt
     9601 gccaccataa aattgagcaa agttggtact ggtacctaat atttttgcaa atgcaggcag
     9661 gatggcaacg attgctaaaa atatagaacc cggcaaagtt attttagaca tcacatcatc
     9721 aataaaggat gctgtttgcc tgcctggctt aatgccggga ataaagccac catttttctt
     9781 catatcatca gccatttgat taggattaac cgtgatggca gtatagaagt atgtgaataa
     9841 tataatcaat attgcgaagg tgacattgta ccaaacagaa tggaaatctg ttaaagaatg
     9901 tgcaataccc tgcattgaag atgactcagg aaaaaattgt gctactgtag ccggaataaa
     9961 cataattgcc tgggcaaaga tgattggcat tacacctgcc gcatttacct ttaaaggaat
    10021 atactgacgt acgccaccgt actgtctgtt accaattatt ttttttgcaa actgtaccgg
    10081 tattttacga gtaccttgca ccaataaaat actgattaca acgacagcaa acaaggcaac
    10141 caattcaaca aggaacatga ttagtccgcc acctgtttga ttgagtcgtg ctaaaaactc
    10201 atctcgtaag gcaaatggta atcgggcaat aatacccacc atgataagta atgaaatacc
    10261 atttccgatt cctttttcct gaattcgttc acccagccac atcacaaata aagtacctgc
    10321 aataagaatg atgatagatg taatccacca cattgtcggt gaaaatgcat cagggttggc
    10381 gtatgctaca attgcagact gtgcctgagc ccttaagtta actaaataac ctggtgcctg
    10441 accggcaact accaacaccg taagccaacg tgtaatctga ttcattcggt tacgaccact
    10501 ctccccttct ttttgcattt tctgaaagta gggaacggca attcctaata tttgcacaac
    10561 aattgaggcc gagatatatg gcataattcc cagtgcaacg attgatgcct gtgaaaaggc
    10621 tccacctgca aacatatcca ataaaccaaa aatcccctga gatgcctgat tctttaaatt
    10681 cccaagctgc tgagggtcca cacccggcaa tacaatatgc gaggcaagac gataaatggc
    10741 aatataaaaa aaggtgttga ggagtttagt tctaagctct tcaatcttga atatgttcct
    10801 taatgtttga atcagttctt tcattatttt tcttgaatgg tggttgtgcc tccagcggct
    10861 tcaattgcat tttttgcagt atctgaaaat gcatgtgctt ttacattcat tttaactttc
    10921 aattcgccac gtccgagtat ttttaccaac tcacgctttg aaatcaatcc tttttcgcaa
    10981 agaatgctat gatcaatttc tgttaaacca aatttctcat ttagtttctg aagtacatct
    11041 aagttgatac ctgtatattc aactctgaag aaatttttga atcctgattt aggtaccctt
    11101 ctctgaaggg gcatctgacc gccttcaaaa ccaaatttgc gcgaatatcc tgagcgtgat
    11161 tgtgctcctt tatgaccacg tgtagatgtt ccaccatgac cagagccttc accgcgacct
    11221 attcttttgc ttttctttgt tgaacctttg gcaggtttta aattacttaa attcataacg
    11281 ctttttctta tttgatttct gttacctcaa ccagatgtct aactttttcg atcattcctt
    11341 ttatctgatc agtgttttct ttttctacag aatgatatac ttttcttaaa cctaaagctt
    11401 ttagtgttag cttctgattt ttattgcgat caattccgct ttttacttgt ttgataatta
    11461 cttttcccat gagtataata ttacccgtta aatactttgt ttaaatctac accacgctga
    11521 ttggctacgg ttacagcatc acgcatcata gctaaagcag taatggtagc ttttaccacg
    11581 ttatgtggat ttgaagatcc ttttgactta gctaacacat cacttatgcc cacactttca
    11641 agaactgcac gcatggcacc gcctgcaatt acacccgttc ctggagctgc cggctttaaa
    11701 aacacttttg caccaccgaa agcagcatct tgctcatggg gaacagtagt tttatgaact
    11761 gtaaccttca ctaagttctt ctttgcatca tcaatagctt tggtaattgc atcagtaacc
    11821 tcttttgctt ttcctaagcc ataacctaca acacctttct catcaccaac cacaacaatg
    11881 gctgcaaaag aaaatgttct tccacctttg gtaaccttag ttactctgtt tactgctacg
    11941 agcctgtcct ttaattcgat ttcgcccggt ttaactcttt tatttgctgt tgctatcatt
    12001 gtttcgtttt agaattttaa tccgccttct cttgcaccat cagccaatgc ttttacacgg
    12061 ccatgaaaca aatatccacc tctgtcgaat gttactttgg ttataccttt agctgctgct
    12121 ctttcagcaa tcattttgcc aactgaagtt gaaatttcaa ccttagtacc tttagctgca
    12181 ccattttcag cagatgaagc tgctgccaat gtctggccgg caacatcatc aattatctgt
    12241 gcataaatct gcttattact tctgaaaaca gacaatcttg gctgctcatt agtacctttg
    12301 atttttccgc gaatacgttt gcggatttta agcctgcgtg aatctttaga attcttcatt
    12361 ttcttacttt acttattttt tagcagctga ttttccagct tttcttctca atacttcacc
    12421 cacaaacttg ataccttttc ctttgtatgg ttcaggctta cgaagtgatc ttatttttgc
    12481 agcaacctgt ccgataagtt gtttatcgtg actttcaaga atgatggtag gattgctacc
    12541 acgctctgtc tttgtgctta ctttaacttc tgttggcagt tcaaatacca catggtgaga
    12601 atgaccaagt actaaatcaa gcatctgacc ttgattactg gcacggtatc ctacacccac
    12661 cagttcctgc tcaatcttat atccttgtga tactccttta atcatgttag caagcaatga
    12721 acgatacaag ccatgcaatg acttatgacg tttttgctct gtgctgcgtt taacaacaac
    12781 tttattctct tccatttcta aggtaatccc atctgcaatt gtttgagata atgtaccctt
    12841 tggacctttt acagaaactg tatttccttg tacattaacc tgaacacctg atggtactga
    12901 aaccggtaat ttccctattc gtgacatgct ttcttaaatt aataaatata acataaaact
    12961 tcaccaccaa catgcagctg acgtgcttct ttgtcagtca taattccttt gcttgtagag
    13021 atgattgcca caccaagacc attaataatt cttggtaaac gatcatgact aacatacttt
    13081 cttaaacccg gacggctaac acgcttaagg cgcgtaattg ccggcatctt ggttacagga
    13141 ttgtatttta aagcaacttt aatatttccc tgcttattgt cagtttcttc aaacttatag
    13201 tttgcaatat accctttgtc aaaaagtatt ttggtaattt cttttttagt atttgatgca
    13261 ggtacttcta cgatgcggtg attggcctta acggcattac gcaaacgtgt caaataatct
    13321 gatatcggat cggtcatttt aattattttt ctaattattg tttaatatta ccaactggct
    13381 tttgttactc cggcaatttt tccttctaaa gccattttgc gaaacagtac gcgtgaaatt
    13441 ccaaactgac gcatataacc tctcggacga cctgttatca tacaacggtt atgtaaacga
    13501 acaggtgatg aattacgagg caataatgcc aatgcctgat aatcgccagc ttctttcaat
    13561 gctgcacgtt tagctgcata tttctcaact aactgttctc tttttctgct tcttgctttt
    13621 attgattctt ttgccatttt ttcgaattat tttttagtta gaaccggttc tgaatggtag
    13681 tccgaattct ttaagtaact gcattgcttc attatctgtt ttagctgtag ttacaaatgt
    13741 gatatccatt ccacgaatct tacttacttt gtcaatgttg atttcaggga aaatgatatg
    13801 ctcggtaatg ccaagtgtat aatttccttt accatcaaaa cctttgttgt tgattcctct
    13861 gaagtcacgg atacgtggca gcgatacaga aaccaaacga tcaaggaatt catacatctg
    13921 ctcatcacgc aaagtcactc tcactccaat aggaacacct tttcttactt taaagttaga
    13981 aatgtctttc ttagcataag ttgcaacggc tttctgtcct gctatagctg tcatttctgc
    14041 tacacaagca tcaacaattt tcttatcgct aacagcatcg ccaataccct gactaaggca
    14101 tatttttgat aacttaggca cttgcatgat actcttatac tgaaaatgat ttttcagtgc
    14161 tgggatgatt tcctttttat acttgccttt taggcgcggt gtgtaactca ttattttatt
    14221 acctcccctg atttttttgc gtaacgaact aatttacctt catctgtttt acgacctaca
    14281 cgtgttggct ttcctgattt aggatctata agcatgaggt ttgaaacatt aaccggagct
    14341 tcttttttta cgatgccacc ttgcgtattt tgtgcatttg ggcgtgtatg tttagaaacg
    14401 atatttacat tctctacact tgccctgttg gtttcaggga aaacctcaag tacacggcct
    14461 tgctttcctt ttgactcacc ggcaataacc ttaacaatat cgcctttttt tatctttagt
    14521 tttgacatga ttctgatttt aaaaattaaa ttacttcagg tgccaatgaa acgattttca
    14581 taaactgttt ttcgcgcagt tcacgggcta caggtccgaa aatacgtgta cctctgagtt
    14641 catctgcctg attgagtaac actactgcat tatcatcgaa acggatataa gatccgtcat
    14701 ttcgtcttac ttccttgctt actcttacaa ccacagcttt gcttacagca ccttttttaa
    14761 tatttcctgc aggtaaagca ttctttacgg ttactacaat tttatctccc aatgaagcat
    14821 atcttttgcg tgtaccgccc aacacgcgga tacacaaaac ttcttttgct ccgctgttat
    14881 ctgctactgt taatcttgat tctgactgta tcatctttta tttcttttca atattctgcc
    14941 ttttagtatt ggcgagaaat tttaattatt ttgctttttc aagaatttca actaatctcc
    15001 aacgttttgt tttgctgatg ggtctggttt ccataatcaa aacacggtcg ccactaccac
    15061 aggcattagc ttcatcatgt acatgaaact ttttagtctt attgataaac tttccgtaca
    15121 tcggatgttt aaacttcttt tcttcagcca caacaacagt cttttgcatt ttaatgctgg
    15181 taaccacacc tatttttgtt ttgcgtaatg ctctttcagt actcattgaa taatttattt
    15241 tgatgtttgt gacattcttt tgtttaactc tgttttcagc cttgcaataa gtttgcgagt
    15301 gctgcggatt ttcatcggat tatccattgg agaaacggca tgctgcattt tatgtttctg
    15361 taaattatcc gcttcttcct taatgcgagc ttttacttct tctgttgtta aatctctaac
    15421 tattgactgt ttcatcgttt ttcaaatttt aatattaagc agctgaataa tctctgcgaa
    15481 taacaaactt ggttataacc ggtaattttt gtgctgccaa acgcattgct tcttttgctg
    15541 tttctacaga cacaccatct gcttcaaaaa gcatggttcc aggttttact tgggctatcc
    15601 aatattccgg tgctccttta cctttaccca tacgaacctc agcaggctta cgtgtaacag
    15661 gcttgtccgg gaaaatacgt atccaggttt gtccttcacg cttcataaaa cgggtaaggg
    15721 caacacgggc tgcttctatc tgacgtgaag taagttgtgc attttccagt gatttaattc
    15781 caaatgatcc gaaggcaatc tgcgatccgc gggtagcatt accacggttg cgcatctttt
    15841 gctgctttct gaattttgtc tttttcggtt gtaacattgc taattaacct taattctttt
    15901 tttaataatt atttcttctc tcctcttgga cgtccgcctc ttctaccgcc acgttcatta
    15961 ccgccacgtt ctctttcctg tcttctttcg ccacctgcat tttgtttagg tgttgagttc
    16021 attccaacat taggcgaaag gtctctttta ccaaaaactt cacctttaca aatccaaact
    16081 tttaccccta tttttccata agatgtctgt gcatctgcaa tggcaaaatc aatatcagca
    16141 cggaatgtgt gtaatggaat acgtccatct ttgtactgtt ctgtacgagc catctcagca
    16201 ccaccgatac ggcctgcaca cataatctta ataccttctg cacccatacg catgcttgaa
    16261 gcgatagcca ttttaatagc tcttctgtat gaaatacggg cttctatctg tttggcaacg
    16321 ccatctgcaa ctaactgtgc atcaagttca ggacgcttga tttcgaaaat atttatctgt
    16381 acttctttct tggtaatctt cttaatttct tctttaagct tatctacctc tgatccacct
    16441 ttaccgataa cgatacccgg acgtgcagta tgaatagttg cagtaataag ttttagtgtg
    16501 cgctcaataa taatacgggc aactccacct tttgccaaac gtgcatgcag atagttacgg
    16561 attttttcgt cctcaactaa acggtcagag tagttctttc caccatacca gtttgagtcc
    16621 catccacgga tgattcccag tctatttcct atcgggtgtg ctttttgtcc catttatgct
    16681 tctgtatttt cagttacttg ttgtgtattt tctacttttt tattgacagt gtcaacgaat
    16741 aaggtaacgt ggtttgaacg cttacgaatt ctgtaacctc taccttgtgg tgcaggtcgc
    16801 aatcttttaa gctgacgagc gctgtccaca taaacttttg atacaactaa tggctgatct
    16861 tcgatgcgaa caccttcgtt ttttgcctgc cagttattga ttgctgataa caacaattta
    16921 tacatgttgc cggcagcttc tttcttactg aatttaagca atcccaaagc aagatcaact
    16981 ttcttacctc tgatcatgtc agctaccagt ctcatttttc taggagaggt aggacaatta
    17041 ttcaacctgg caatgtaggt tgttttcatt tcttctttgc gggcatctgc cgctattttc
    17101 tttcttgctc ccattatttc ttatctttat tacctgcatg gcctctgaaa gtacgggttg
    17161 gtgcaaattc gcctaatttg tgtccaacca tattttctgt tacatataca gggataaact
    17221 tattaccatt gtgaactgca aatgtatgtc caacaaaatc gggtgatatc aaagagcgtc
    17281 tgctccatgt tttgattacc gtcttctttt taccctcact cattgacgca acttttcctt
    17341 ccagtttagg atcaatgaag gggccttttt ttagtgaacg actcatttat attttattat
    17401 tagcttgaag cgtattattt aattatggag aattatttct ttcttctttc tatgatatgt
    17461 ttattgcttt gatttttcag cttacgggtt ttctttcctt tagctaacaa acctttgcgt
    17521 gaacgtggat gacctcctga agcacgacct tcgccaccac ccattgggtg atcaacaggg
    17581 ttcattgcta caccacgcac tcttggtctt ctgcctttcc agcggttgcg gcctgccttt
    17641 cctgcacttt ctttattatg gtctgaattt gaaacagaac caatagttgc aacacaggtt
    17701 gaaagcacca ttcttgtttc accggaaggc atcttcagga ttgcatactt tccatcacga
    17761 gctgttaact gtgcataaga tccggcactt cttgccattt ttcctccctg acccggacgc
    17821 aattctatat tatgaactat tgtgcctaat ggcatgtctt tcaacatcat agcattacct
    17881 acttcaggtg ttgctttatc tcctgaaaga atagcctgac caacggtcaa tcctgcagga
    17941 gcaagaatat atcttttttc accatcagca taatgaacta atgcaatacg tgcagtgcgg
    18001 ttaggatcgt actcaatagt tgctacttta ccgggaacac cgtctttatt acgcttgaag
    18061 tcaattattc tatataactt acggtgacct cctccaatat agcgcatggt cattttacct
    18121 gaattatttc ttccaccttt gcgattgata cgctctgtca aagctttttc aggttcgtta
    18181 gttgttatat tagaataatc tggcgcaatt aaaaagcgct gacctggagt aatcggatta
    18241 aatttcttga ttgccatttt tcaattttta tatgcttgcg taaaaatcaa ttgtttcgcc
    18301 cttagctaca gttacgtaag ctttcttttg cccacctttt ttaccttgca gaatacctgc
    18361 tttggtgaaa cgtgtttttg attttacagc attattaact gtatttacac tcacaacatt
    18421 gacattataa agcttctcga tagcagcttt gatttcaatt ttgtttgcgt ttttatctac
    18481 gataaacgca tattgcccga gtttctcgga cttctgcgtc attttttcag taataactgg
    18541 cttctttaag atgctcattt gtttaatatt ttttcaatta ccggcaaagc gccttcttcc
    18601 aacaccaaat tttgcgcatt tagaatatcg taagtgttta tttctgaagc acttaatact
    18661 ttgctgcgtt gcaaatttcg ggaggacaaa aataaatttt tattggtatc tccggtaacc
    18721 actaaaactt ttttattagc aagttccaat tgcttcaaaa tgtttaccac ttcactggtt
    18781 tttggagctt ccattgtcac attctcaacg atggtaatat tgttgtcttt tgccttatag
    18841 ctaagagcag atatacgagc aagtcgttta actttcttat tcagcttgaa tgagtaatcg
    18901 cgtggctgag gaccgaaaac acgacctcca ccaacgaaaa gtggcgattt catacttcca
    18961 gcacgtgcac caccggttcc tttctgacgt ttcaacttct tagttgttcc tgaaacttcg
    19021 tttcgttgct ttgacttatg tgtaccctga cgtgcgttag ccataatgct ttttacatca
    19081 agccatattg catggtcatt tggtgtggca gcaaagattt cgctgttaag cgttactttt
    19141 cttccagtag cctgaccgtt tctttgttta atttctaatt ccatttcgct taaaattcta
    19201 ctttaatata tgaaccttta gcacctggta cagcgccttt tatgattaag atgttgtgct
    19261 caggcataac ttttaagatt tgcagattct gaatctttac ctgctcatcg cccatgtgac
    19321 ctgtcaatct ttttcccgga agtactcgtg atggaaaaga tgatgcaccc atagaaccgg
    19381 gggctctcaa tctgttgtgc tgaccgtgag ttgcacctcc aacaccggca aagttgtgtc
    19441 gcttaataac accctgaaag cctttaccct ttgaggttcc aacaacatca caaaattcgc
    19501 cttcgggaca gatatcaact ttgataacat cgccaagttt tacctcagta gtaaagcctt
    19561 taaactctac taccttacgc ttaaatgagc tgtttgattt tttaaaatgg ccttgcaacg
    19621 cttttgttgt atgcttttct tttttgtcat caaaagctaa ctgaacggca tcatagccat
    19681 cggttgctac tgatttaacc tgggttacaa cacatggtcc tgcttcaatt actgtgcaag
    19741 caatgttttt accctcagca ttaaacatgc tggtcattcc tattttcttt cctattattc
    19801 cggacattgt tctttacttt aagggtttaa ctacactttt atttcaacct caacaccact
    19861 tggtaactcc agtttcatga gcgcgtcaac agttttggag gttgagctat atatatccaa
    19921 caaacgttta taagagcata actgaaactg ttctcttgcc tttttattta cgtgtggcga
    19981 gcgaagtact gtatatatct tcttctcagt tggaagagga atgggtccgc tgatcactgc
    20041 tcccgtagtc ttaacggttt ttacgatttt ctctgctgac ttgtcaacga ggttgtaatc
    20101 gtaagatttg agctttattc ggattcgctg attcatttat ttattattta atttcttttt
    20161 aaaatctaca attaagcttt ttctaattta ccttttactt tggcaattac ttcttcggca
    20221 atagcgcgag gacactctgt atagtgcgag aactccatgc ttgacgaggc ccgacctgaa
    20281 gtaatggtac gcagtgttgt tacgtatcca aacatttcag agagtggaac ttttgctttt
    20341 attacctgtg ctcctgcacg tgtgtccatt ccttccatct gtccacgtct tctatttaga
    20401 tcacccataa catcacccat atattcatca ggagtaacaa cttctacctt catgattggc
    20461 tcaagcaata ttggttttgc ttttgcacaa gcttctttat aagcaagttt ggcagcaatt
    20521 tcaaacgaca aggcatcaga gtcaactgcg tggaatgaac catcagtcag aactacttta
    20581 aggctgctta atggataacc ggcaagtaca ccattgacca ttgctgcttt aaatcctttt
    20641 tcgattgaag gaataaattc ttttggtata ttacctcctg taatctgatt tacaaattgc
    20701 agatttgact tatttgcaac cggatcaaaa tcttcgtcaa cggggctaag tgtaaattgt
    20761 atatcggcaa acttaccacg accaccggtt tgtttcttgt aaacctcgcg atgtgataca
    20821 ctacctgtta ttgcttcttt ataagaaacc tcaggtgcac cctgattaca ttctacttta
    20881 aattctctct tcagacggtc aacaataatt tcaaggtgaa gttcacccat tccgctaatg
    20941 attgtctgtc ctgtttcttc gtcagttttt acacggaaag taggatcttc ttcagctaat
    21001 ttattcaatg cagttcccaa cttttctgtg tcaacctgag tttttggctc aatagccaaa
    21061 ccgataactg cttcaggaaa ttgcattgct tcaagtacta taggtgcatt ttctgcacag
    21121 agagtatcac cggtcttgat atctttaaat cctacagcag caccaatatc accggcctcg
    21181 ataaaaggaa tctggttttg cttattggca tgcatctgga agatacgaga aatacgctct
    21241 ttattacctg aacgagagtt tagcacataa gaacctgact ccaaacgtcc ggaataagca
    21301 cggaaataag ctaagcgacc tacgaaagga tctgttgcaa tcttaaatgc caaggctgta
    21361 aatggctctt ttacatcagg cttacggctt acaggctcac cattgtcagg gtttgttcct
    21421 gtaatattat ctttatcaat tggacttggc aatagttcca ttaccaggtc aagcattgtt
    21481 tgaacacctt tattcttaaa tgatgatcca caaaccatag gaaccacttt tcctgcaata
    21541 catgattttc ttaatgcatc cagaatttcg cgttctgtaa tgctatttgg gtcatcgaaa
    21601 tacttctcca tcaatttatc gtcaaattca gcgatagatt ccagaagttt ctctcttaag
    21661 ctttttgctt cttctaatat ttctgcagga ataggcactt ctgtaaatgt cattccctga
    21721 tcgtgttcat tccaaacgat acctttaaag ttaattaaat caactacacc tttaaagttc
    21781 tcttctgctc caataggtaa ttgtaatggc actgcattac cgccaagcat ttcacgaacc
    21841 tgcttaacca catttaaaaa gtcggcaccg gcacggtcca ttttgttcac aaaacaaagt
    21901 cttgtgacgt tatagttatt cgctaagcgc cagttggttt ctgattgagg ttcaacacca
    21961 tcaacagcag aaaacaagaa taccaaacca tccagaatac gtagtgaacg gtttacctca
    22021 acggtaaagt caacgtgtcc gggagtatca ataatgttta ctttatattt attgttgcgg
    22081 taattccaaa agcaagtagt tgcagctgat gtaatggtaa taccacgctc ctgctcctga
    22141 accatccagt ccattgttgc agcaccatcg tgaacctcgc ctaacttatg gtttacacct
    22201 gtatagtaaa ggatacgctc tgttgtagta gtcttacctg catcaatgtg tgcagcaata
    22261 ccaatatttc tagtaaatct taaatcgttt gccatttgaa tagtgtctct ctgcttttat
    22321 actttaaaat gtgagaatgc cttgttagct tcagccatac ggtgtgtatc atctttcttc
    22381 tttactgctg cgccttcgcc tttagctgct gctattattt ctccggccaa gcggtcagcc
    22441 atagacttct cattacgtga gcgtgcatat cctaccaacc acttaatgcc caatgccgtt
    22501 ctgcgtgtag gacgaacttc tgatggtatc tgaaatgtag ctccaccaac tctgcggctg
    22561 cgaacctcta ccaatggcat aacattattt aatgcttttc tccattgctc caaaccgtcc
    22621 tcctgtgttt tttcagtaac cttttctaaa gcatcgtaaa aaatctgata ggcagttcct
    22681 tttttaccgg actccatcat accatttacg aaacgtgtta cctgttgatc attatatctt
    22741 ggatcaggca ataaaatcct tttcttgggt ttcgactttc tcatctatga aattttaaac
    22801 tgaaactttt ctgattattt tttagctgca ggggcaccgg ctttaggctt cttggttccg
    22861 tatttacttc tgcgctgatt acgtccttct acacctgagg tatctaatgc accacgcaca
    22921 atatggtatc taactccggg aaggtctttt accctgccgc cacggattaa tacaatagag
    22981 tgttcctgga gattgtgacc ttctccggga atatatgctg taacttcaaa tttgttggtt
    23041 aaacgtacac gggccacctt gcgtaatgca gagtttggct tctttggtgt tgttgtgtaa
    23101 acacgtgtac aaacccctct tctctggggg caggatgtta aggccgggga cttgcttttg
    23161 tcctcaattt tagagcggcc tatgcgtatt aattgttgta ttgtaggcat cttaattatc
    23221 caatatgtta atcccaactt tttttgggac tgcaaatgta gagattcttt ttagaattac
    23281 aactaattga aaaagatttt tttaagaatt tcttcaaatg gctatcgtgc tggtaatcaa
    23341 ttatatttgt gaaataatcg aaattgactt tttcatatcc tgacaaatta ccgctacttt
    23401 caggctaaaa atattcttca ttttgttaaa acagtcatca aaactattgt caattaaaca
    23461 tacgagcatt acgaccaacc cttgaagagt cattcactcc aaacccttga agagtcattc
    23521 actacaaaat cggggttagc tgttatgcga catggcttta gtaaaacata ttttttaaag
    23581 gcaagcatgt attttctatt tataaaacaa ccgtgtggct tattgctttg gttttagtaa
    23641 atagtttgag cgtattggat tgaagtatgc catcgcacct gattaatttt cactaacctt
    23701 ttggccatta aaaagctatc aggtgataaa agcctgctta ccgccatgat atttaccaaa
    23761 gtcagtaaaa aaatatctaa ataaaacaca aagtccgtca gacaaaatag gatataaaaa
    23821 aaaattgtac aattgcttta tactgctata tttgtacaat attcagcaag ttttaggtta
    23881 aaacatgctg atatttaaat gttagtggtc atgctatgga gactgcgtac acattaagtt
    23941 tacttctcga ccttttgatt gccgatttga aattgcttag ggtcaattat tggtaggact
    24001 gccccatgta agaaagttcc tttgaaagtg gagaacatta cacctcttgt agttgaaatc
    24061 gaaataatgt tgagcgtttc aatcaactgt tgaggaatat caagttttcc gttatcttca
    24121 atttttatta cttcatcaaa attgacaatc tcaaaaatgc aacttactga caatgcaccc
    24181 aagacaattg agtgatcatc gttcattatt tcaacatgga ctataacaaa tacaagtttg
    24241 ttaggggcat ctgctttgct ttcaatacta atattgaaat tgaaagttgt aatggctgtg
    24301 tttggggctg ccggtaactg caacgaccca tttaacagct caattgccct aatctgcatt
    24361 tcgacattga agtctttatt ttcgatttta gccattttat gcttgatagt attttgtgaa
    24421 ttgtgagtta tttccttgac ttgctaaaag gcaatattca taaacatcaa tgccttgaga
    24481 cattggtgtt gtcataacaa tagttggttg cgttacgaca gttttgacaa caatttcctt
    24541 acgataaata acctgcacct gcttttttcc aaacaatgca gttaggtcaa caccaagagc
    24601 aaaagcaatt tcagttaaca catcagttgt gaaattttga gtgccgctaa accacttagt
    24661 tatttcagat ggatttttta cgactttttc tgcaaactga gacttgttcc aaccttttgc
    24721 tctcatgaaa tcttcaatac gagccgcaag ttgcattttc acttttgtct gctccatttc
    24781 caatggagta acttcgtcta atagtttttg cagttttgaa ctattatact ttcttgcttt
    24841 agttgtctta ttcattgtcg taatttttta aatttccttg taactccgtt ctgtcttttg
    24901 accaatacaa atcaccatca tccattcttt gtaaaatatc ttttgcgtat tcaatcattt
    24961 gatttgcctc ttttgataat ttttcatcat cctgccatgc tctgacacct tctgcctttt
    25021 ctccaccgcc accgagaata actgccacca ttccaaaccg aatacaatac actctcaaat
    25081 tcttttccgg gacatcaaac aatgcacaaa caaagtctcc gtatttccct tcatgttgct
    25141 taaagaacga acttcttgca cctgttgtat gaccaatttg ataaatggtt ttgattatgt
    25201 cttttacttc atttttaaac tcgaccttat actcatccac gaacttctcg aacaaggttt
    25261 catcctcacc cataggaata agtgagtaaa cctttgcttc gctcccgctg tatggctgta
    25321 gttcgacaat ttcgtagttc acttggcgta gaggaggttt taataataac gcatatttta
    25381 aaaatatgtt tcacctataa gtgaaattag actgcaaatg tatgaacata tttttgtcaa
    25441 tagcaagtaa ttctacaaat aatttcactt ttaagtaaaa ttccatattt atgctgacaa
    25501 gttagagtaa ctcaatgaca aaagaagcac gaacgcacaa tagagtaacc tgccccgcca
    25561 gcgggtgctg ccggagtgct accggtagct atcggcatct cacaccacta atcgtaaggg
    25621 tcgcatactt agcggctggc acctcatatt tgtggcattt ttagttgctg tttttctcct
    25681 gaaacctttt gaaattaatt tatatttgta aatgcttttg aattattttt ttcactgact
    25741 gccgttatac cccattctaa aactacagaa aacaagcgat ttgaagtggg gattttgtaa
    25801 atttgaatgt tgatttaagg ctaaaaaata tgtacaacac aattgaaata gacagagata
    25861 acctgacaat tatgggaata aaattctcag acataaaaac tcttgaaagc actgcaaacg
    25921 ctttgggcag taatatgttt gaaggcttta aaccgacacc aaaaggaatt gaaattataa
    25981 gggactatgt gacaggaaaa atatcattgg cagaacttgt ggcatttgcc aaacaaaaag
    26041 cctatgtcta attcctacaa atacatagac cctgaattca gctacacaga ccctaagaca
    26101 ggacttttaa agaatttaca agatattacc gaccctgaca ttttactctt tgttcagagt
    26161 ggtgtggtaa caaaacgact ttaagaactt tatgaaaaac caataaaaat aaagagtatt
    26221 gacagccttt ttgaaattca tagacacttg tttcaagata tttacgcttg ggcaggtaag
    26281 agacgaattg ttgaaataag caaagatggg aaacaatttt tcccgacttc acaatttgac
    26341 aatgctttca gacacattga ccaattaatt gccgatttca agaaaattcc gggagagaat
    26401 aaaaatcagt tagccgataa attagcagaa attttagata acttaaacta cttacatcca
    26461 tttagagaag gaaacggacg aacgcaaaga gaatttttaa gactgttggc attggaaaaa
    26521 ggcttaacac taaatctcaa cccaccagat aacaaaagtg tttacgaacg atatatgaaa
    26581 ggaacaattg aaagtgaggt aaaaactttg gcagaattaa tttttgaatt gattgatgag
    26641 aatgaaaaat agaagaacgg tgcataatcg aataggcagc tccaccagaa actgacggag
    26701 atctgccgat agctatcggc atctcacacc actaagcgta cgggtcgcat gcttagcggc
    26761 tgacaccttg tatttgtggc atttttattt gctgtttttc tccttaaaac tattggaatt
    26821 aatttatatt tgtaaatgct tttgaaatat ttttttcata aactgacgtt gccagctatt
    26881 atttggcggc agtgctaacg ccaacctttg tggttctatt gaacagtaac acatgacaac
    26941 tttttcgaaa aaaacagata tgaatttcgt tgattggggt gaaattgaca tgcccaaagc
    27001 ggtttacaaa taccgaacat ggagtgacaa ataccataag acaatcttga ccaacttgca
    27061 agtattcatg tcgccacctt tgggatttga agacccacac gactgtaaga atttaattcg
    27121 ttacgattta cttacttcaa atgaaatctt ttggaagtat catcaagaat cacttgagcg
    27181 aaatccaagg ttcactccag agcagcatca aggatttgct caatattggt ttgaaagaag
    27241 tccgcttcac gacaaagatt ttattaagca gcaacaggaa aaagatttta ttgaagaaga
    27301 taagcgactt ggtattttaa gcttaaccac aaatccaatc aataattcga tgtgggaaaa
    27361 gtatagcgag aaccattgcg gtttttgcgt tggttttcat tccgaaataa tgtttaagtt
    27421 tttgggtgga ggtggtgcag taaattattg cgatgagttg ccaatcatta agccaactcc
    27481 ttgggatgat tataatatgc aacacttgaa acaagtttat tacaaactta gaaagtggag
    27541 ctttgaagaa gaatacagaa cacagaagtt ttcatatgag ccgttaactg ttcagaatag
    27601 aactattact ttaccatcag aagcatataa agaaataatt tttggtgcaa agatgaatga
    27661 gaaggatatg caagaaatta tttccatttg caaatacaat ctttcacata ttgaattcta
    27721 tcaagctgtg ttgaataact caaacgacaa tgtttcaatt gagaagtata acagccgcta
    27781 acataaagtc aacattacac aacccaataa aagggtaaaa agggatgtaa aacgacctcg
    27841 ttagctgcct tttaacaaag cggctgcttt ttattgcctg tttgtaatgc taaaatcttg
    27901 tggccgtaaa accgaggtaa tacaaactcc tatcactaga tgctgaagta tccaaacact
    27961 atttgtgatt tttccgctat agaaaccaat cacttttgaa cattaacatc ttccaaaaaa
    28021 caaatacagc cccccctatt ctaatccggt ttttacatta taaaacaaaa tgaactgata
    28081 aaaattgtag tttcggtttt ttttaaaatc tatggttcgg aaaattcact ttattttttt
    28141 gctgactcta tggtgcagtg tgttgtctgc acagtctttt aatccatcag agatcagact
    28201 aaacctcgaa aaactgaata ctgttggaac ggtcttatat attgcagccc acccggatga
    28261 cgaaaacact agattgctga cctacctttc aaaagaaaaa catttacgca cgtgctatct
    28321 gagcctgaac agaggagaag gaggacaaaa tctgattgga aaagaacagg gcgctgcact
    28381 tggaattata agaacgaatg aattgttgca ggcaagaaaa attgatggtg cagaacaata
    28441 ctttacacgc gccattgact ttggctacag caaatcgcct gaagaaactt ttaccttctg
    28501 gaatcatgac agtgtgcttg ctgatgtagt atatatcata aggctgttgc agcccgatgt
    28561 tatcataacc cgttttccga ccactggcga aggcggacat gggcatcata cagcaagtgc
    28621 aattttagca ggtgaggctt ttaaattggc tgctgatact aaatatcttc ctgagcaact
    28681 taaatcttta aagccctggc aagcaaaaac actctattgg aacactttta attttggaaa
    28741 caataacaca cagcgtgatg accaattgaa aacagatgtc ggatttttca atacctggtg
    28801 gggaaaagga tgtggcgaaa ttgcagcatt aagccggagt cagcataaga gtcaagggtt
    28861 tggtgtagaa attatcagag ggtcaatgct tgagtattta aaaaacattg atggcgacac
    28921 ttcatgcaca acattatttt gcaaaaacaa tttcagttgg acaagagtta aaggagcaga
    28981 aacaataaag tcccttactg aaacagcctt ggttaaattc gatatgaaaa acccatctgc
    29041 tattttaccc gacttgctaa agctatatga tgagttaaaa aaactacaag ataatcactg
    29101 gaaaaaacaa aaaattaaag agacagaaca gcttattttg gcatgtgcag gtttgtggtt
    29161 tgatgctacc tcgaatcaac cgtatgtaac tcatggtgac agcataaata ttaaactcag
    29221 ttctattgta tattcacaaa ctccaacaac aattgaatca gtttcgtact taccattttc
    29281 ggatacaagc atgagtacaa aacttgatta caataaaatt tggtcgcttg aaaaatcatt
    29341 tgttgtgcca aaaaatattc catacagttt tcattactgg ttgaaaaaca tacctgaatc
    29401 gggtcgttat gttttagaca gtcaaaacat aattgagaag cctgttaacg atgccgtttt
    29461 ttctgtttct gtaggcgtaa aaatttcaga ccatacatta actttcaatg ttcctgttcg
    29521 atacaaatgg attgatccgg tagaaggaga aaagtacagg acaaccagtg tagtccctcc
    29581 tattgctgta aactttaatg aaaatgtttg ccttttaata aacaacaaac ccggtaaact
    29641 gaaactaaca gtacgaagtc tgagtaaatc ctttactggt aaaattaaat taaacgtgcc
    29701 ttcaggttat atagctaatc cctctgaatt tgcagttaca gaattattaa aaaacgaacg
    29761 acttattttt aatactacta tacaacaaga cgtaaaagct gatgttgatc aaatcaatgc
    29821 caatcaacat ttacaagtca gtattttacc aactaattca gaagcatatt ctttgcagga
    29881 aatcaattac gagcatatcc cctaccaata ttatttcaaa ccttcggaag ttaaaattgt
    29941 gccactggaa ttgcagacct cttccaatag aataggatac attgaaggtg caggcgatga
    30001 agtgatgcag tgtcttcaga atgcaggtta taatatttct gttcttaatg aagaaaatct
    30061 tcgcaatgat aatctgtcaa attacagtgt aattattgca ggcataagag cctataacac
    30121 caacgactgg ctgataggtt ataaaaacaa actcatggac tttgtttcgt ccggaggcac
    30181 ctacattgtt caatacaata ccaacaacaa cttaggtaaa cttggcaata acatcggacc
    30241 ttatcctttt aaaatttcaa gaaaaagagt tactgatgaa tttgcagaaa tgcgttttac
    30301 agaacctgct aacaaaacat taaatactcc aaataaaata actgcaaatg attttcgagg
    30361 atgggttcag gagcgtggaa tatattttgc agaagagatt gacccaaagt atcagacacc
    30421 tttaagctgc aatgacaaag gcgaaagtcc attgaatgga agtctgataa ttgcacaatc
    30481 aggcaaaggt cattttgttt atacgggact tgcttttttc aggcaattgc cggcaggtgt
    30541 gcccggtgcg taccgtttat tttctaattt aattgcatta ccctccggtc aggaaaaata
    30601 aattttataa catcgccaat accattaccg ttatcctgac ataaatatca tgggaaaaga
    30661 taagctgaaa cgatttgcag aaatgcagac ttttgagaga gtaattcaac cggaaataaa
    30721 ctaccactca ccggattttg agctgaaagg aaagtggaac gaaacaattt ttcaaaaacc
    30781 acaacctata gtcttggaaa ttggttgcgg caaaggtgaa tacactgttg ctttggcaca
    30841 acgctatcag caaaaaaact ttattggcat tgacataaaa ggtgcaagaa tatggcgtgg
    30901 tgcaaaaacc attaacgaag aaaaaataat gaatgctgct tttctgagaa taagaatgga
    30961 aaccatagaa aagttttttg ccgcgaatga agtaagtgaa gtttggataa cctttcccga
    31021 cccgcagcca aaggaatcga aagaaaacaa aagattaacc tccccccttt ttctaaaaag
    31081 atacagcact ttcttaatac ccaatggcat tgtacattta aagactgaca acaaagattt
    31141 gtatgaattt acactgcaaa ccgtaaataa tgccggtcat attatattaa ataaaacaac
    31201 agatttatat aaagaatcga agatgcccgt tgacatggac atacaaacca cctacgaaaa
    31261 aaaatattta gcagctaagc agcctatctt ctatctttca ttccaattga acgaaaaaag
    31321 ataagcgaca ccattaaatc tcatcccaaa attcggcagc tcttctggta tgtggaatca
    31381 caatggttcc acccacaata ttggctacag caaacacttc ataaatctgc tctgtagtaa
    31441 caccattttg tttacattga ataagatgat actttatgca atcatcacag cgcagcacca
    31501 tgctggctac aagtccaagc atttctttgg tctgaaccgg caatgctcca tccatatagg
    31561 tattggcatc aagattatag aggcgtttca gaaccagatt atcagattcg agaatgcgct
    31621 tattcatttt agcacgatag ctgttaaact catctatact tttgctcatg tgatttcttt
    31681 ttattcgttt ttacgctcct gccttgctac tacaacagaa acaaatatgc taaattcgta
    31741 aagcagaaac aaaggaatag ccaccagcat ctgactggta acatctggcg atggtgttat
    31801 taatgcagca acaacaaggt ttacaataaa agcatgtttg cggtagcgcc tcataaactg
    31861 cggactaatg atacctattt tggtaaggaa aaatacaagt atcggcagct cgaaaactgc
    31921 tccgcaagcc aatgtcataa tgcttacaat actgatgtac gaatcgagtg tgatggcatt
    31981 ctgcacctct tcgctcacct tataacttcc caaaaagtta atagacaacg gtgccagaat
    32041 aaaatatcca aaagccactc cgcagaaaaa caaaaacgaa gcagaaaaga ctatacctct
    32101 ggtatatttc ttctctttgg cagacaatgc aggttttaca aaacgccata tctcccaaag
    32161 caaataaggg aatcctaaaa tcaggcctgc aattccggca gctaccatgt gtgtagtaaa
    32221 ctgcccggcc atggtggtac ttatcaggcg aaatggtata tcggtaatac ataaatctat
    32281 tgaccatttt tccgaaagca tacaaagtaa cctgtacgta ataaaatgag ggttcttggg
    32341 tccgaaaatt atcttatcga acaagatgtc cttatatata aatgctacaa cggctaaaac
    32401 aaaaatggcc aatgttgcac gcaccaaatg ccaacgcagg gcttcaaggt gatccagaaa
    32461 attcatttct gagttagggt cgggtttcct tttaaaaaac atttctttga ttaaagatta
    32521 gaaaatgcct tctttaagca aatcgtgaag gtgaacaaaa ccaaggtact tccctttatc
    32581 agccacaacc aactgagtaa tattatttga cttcatcaga acaaaagcat cggcagccat
    32641 ggtgtcgggg ctaacggttt taggctttga ggtcataata tcttttgctt ttgttgccag
    32701 cgtgtcacca ctcctgtgaa gcatccttct aaggtcgcca tcggtaatta tacctttaag
    32761 cttacctttt tctacaacgg cagttgcccc aaggcggctt cccgatattt cgacaatcac
    32821 cttttttagg tcatcggtgg gctttacctg cggactggca ttgtttttat aaacatcacc
    32881 aactttcagg tacaatcttt ttcccaatgc cccaccggga tgatagcgtg caaaatcctt
    32941 acttgaaaac cccctgtttt cgagcagaca cacagccagg gcatcgccca tagccaattg
    33001 tgccgtagtg cttgttgtgg gagctaagtt atgtggacat gcttcgcggc ttacggtagt
    33061 gttaagaatg taatgcgcct gttgtgccag gtaagaagaa gtgtcgccaa ccatagcgat
    33121 aagtttattt ccggccaacc ttagcaaggg tgccagcacc tttatttccg gtgtgttgcc
    33181 acttttggaa atgcaaatca ctacatcgtc cttttggatt accccaaggt cgccatgaac
    33241 ggcatcggca gcatgcataa acacggctgg tgtgcccgta ctgtttagcg tggctgttat
    33301 tttctgagct ataatggcac ttttaccaat tcctgtacaa actaccctac ctttcgataa
    33361 atatattaat tttacagtct caaaaaaatc atggtcaata tattgttgca agccactaac
    33421 agccgcagac tccatctgaa gcgtcttttt ggcaatttta attatttgtt gagctgattt
    33481 caatctgatg tatttctgat gtaattttga aaattattat ttgttgcaat attattgaac
    33541 aaaaatatct attttagtta actcaaattt ttaattggta tattaaataa aatgttagca
    33601 gacacaaaga aaaaaaaatc tgtaaaagac aagaatgaaa aaaccgacac aaagactaaa
    33661 gtgaaggtga aagaaaaagt agttgaaaag aaaactaagg gcaccgaaaa agccaagccg
    33721 gttgtaaaaa ccatagttgc tgcctctaat aagcagaagc ctaccgatgc gcgactaaag
    33781 gatgccttat tcaaatattt tggattcgac acttttaagg gtgagcaaga agaaattatt
    33841 caaaccgtgt tgcgtggtga aaactgtttc gttattatgc ctaccggtgg cggcaaatct
    33901 atgtgctatc agttgccggc attgatgatg ggtggtactg ccattgtagt ttcgccatta
    33961 attgcactga tgaaaaatca ggttgatgca ttgaggggat ttaataccga tgaagctgtg
    34021 gcgcatttcc tcaactcatc actcaacaaa acagagattg ctcaggtaaa aaaagatatc
    34081 aagtcaggca aaacaaaatt gctttatgtt gcgcccgaat ctttaactaa agaagagaac
    34141 gttgctttct ttaaagaaat taaagtttct ttcttagcca tagacgaagc tcactgtatt
    34201 tcagaatggg gacatgattt ccgccctgaa taccgtaggt taaaaccaat catagaagca
    34261 cttggaaaca ttcctgtcat tgcgcttact gcaacagcca cagaaaaagt ttcgctcgac
    34321 attcagaaga atttaggtat gatggagtca aggttgttca aagcatcctt caacagacct
    34381 aatctttatt atgaagtacg accaaaagtt gaagtactaa aagagattat caaatttgtt
    34441 aaaaaccact caggcaaatc aggaatcatc tactgcctca gcagaaaaaa agttgaagaa
    34501 gttgccagca cactcaacgt aaatggtatc aaagccctac cctaccatgc aggtctggat
    34561 gcccccgttc gtgccgaaac acaggacaag ttcctgatgg aaaaaattga tgtc
//
LOCUS       DAOQNP010000025        32866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_432_length_32866_cov_9.197129, whole genome shotgun sequence.
ACCESSION   DAOQNP010000025 DAOQNP010000000
VERSION     DAOQNP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 32866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32866
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_432_length_32866_cov_9.197129"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKY09_05345"
     CDS             complement(<1..253)
                     /locus_tag="PKY09_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12314.1"
                     /translation="MHTIEQLEEMLAEHKEWQGKINFYRAEIDRMKTDLASAVMTDRN
                     HYNMPNVEHYQNQFILQRDILDIMRHDFKQHENKIEAHSA"
     gene            788..1234
                     /locus_tag="PKY09_05350"
     CDS             788..1234
                     /locus_tag="PKY09_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12315.1"
                     /translation="MKRIAEIALMSMCVLSVCKCTFVNAQSNIAERKYRVIAYKQGAN
                     EITSMSNETTVTPNMAIYVPNSFTPNGDGINETFGAYSEGVKDFTMQVYDRWGAMIFE
                     SSDINKRWDGMYKGQIAQQGSYVYKIQAKAINGRILTKKGTVNLIM"
     gene            1429..2166
                     /locus_tag="PKY09_05355"
     CDS             1429..2166
                     /locus_tag="PKY09_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12316.1"
                     /translation="MKHIAKLLWLTFLIPLLSSTASAQDSEEETSRWQIGLTAGINIP
                     LSDFADKLDENFKSGYAILGATSRLRASYHPNSKLYIAADYVWFYNPVNATELLNSLV
                     ETNPPGPRYKTTTYPWKVNSLLLGLGTTLNFNEQTDATLRGMLGFANGKYSKRIYQSF
                     DGVKTLQVTENASSVLDIALNLGFEVKYCFHKNIGVMFAGDYFRTEFNYDDVYLIYSE
                     TGEKRSTGAYSQPVDVFQFSAGIFTKF"
     gene            2434..7662
                     /locus_tag="PKY09_05360"
     CDS             2434..7662
                     /locus_tag="PKY09_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12317.1"
                     /translation="MIKSTLISITRSIVAAFLLAVPTCLVAQNYLPKSYYTFEGSSPM
                     KDSMGTFNLDPNYYQSNYQILTNTSGGVGKYMKLDSSSTIIRGGVLAIDSAVTFEFIF
                     KPGMNFGTTVFAKRMDGALELRMTYPAINFMTEVKAANGSPIYDDFEIDLNGIGRASY
                     SYYVDDNWHHIVFKYNAKAGIKEVWVDGQLPAGFSKTIAGGSFAQSGNRDYMLNSQAD
                     YYKYQGAIDEIAVYYNSLGGNSIYAHYLNFMAGKHYSFANPTVPAPTPSPVTAGININ
                     DFAPGHPSPSLTPIEQLKNFPTPRYKPGHTLFPNNPFVGYYYLSGYQTYETVYSKAVA
                     NSVTLQTDFAKNFNYTIMAAENTGAYSDYSNPTTFSYAWINLANSYPQYKVSALSYWP
                     QLRPTDAGYKSNTAYATCGCLPDKYYLKNSSNQYLNPNGGTGSTKYLSPAGAPDSLVK
                     DGLTQKFYMQQLLTKLSRPIDYILDNGEVIPFLYYTNVMQSDPSVVADKNSSGLDWFT
                     YEGRRAATKIKAYRDQFMGLSGLSNTKFAYYQLDGQPTWNLKWSEVRTVNSAMNGSNY
                     ATGDIYMRWPSNWRYWSGAWKGWQWVVESRREQLLAGDKYFSPAVSPGWDNDETQNVR
                     PAQWLGFLKAVSMTGAEFFTTGYFVTSQPYQNPKNYIWQLVIPPYAQAITSRYEDLFR
                     NGFLMSGDVPNSTTSPTYMAYSFNTGDPGKLVVVRKHNSQNKYAITGSSQPMSNMAGA
                     APDEAVAQINLDGSRIKFKIRRQGSTYIYDNTNTSAPIFYQLDGWHENTYPYYWSKDF
                     KFEAELYDNSNNNVYLKTTRPSGAAAGDFTDYTTAVGFNSVSDLTFNFQPRSTTNYTY
                     YVWVRARSKNSQTTGMTISLDGSNSKQIGCITDANWQWYKYDVNSGAAISFSSVTSLA
                     DHILKITPTNTNLEIDQIHLSTSSNAIYTSPAPCSGVAVPTITASGPVAFCAGGNVNL
                     IASTGLSYLWSNGSTDPYINVTSAGSYVVTVTTAAGTATSSPVTVTVYSLPNATITAN
                     GSTSFCVGGSVTLTASSGSTYLWSSGATTQSITAIGGGNYNVIVTNSNGCSAMSASTA
                     VTVKPVPTATISPSGPTTFASGGSVTLTSSSAGTGGSYKWSPGGMTTQAVTVSTTGNY
                     VVTVTNNSGCSATSAPLSVTVTSGAPTPKITANGPTTFCQGGSVVLTCSAANSYLWSN
                     GATTQSITVTTAGSYTCTATTAGGSATAAAVNVIVTTPTQATITASGSTSFCSGGSVT
                     LNASSGTSYLWSTGATTNSITTSSPGTYTVTITNNGCQSTTNKVVTVYALPTATITAN
                     GSTNLQQGGSVQLTSSSAGSGGSYLWSTGATTQSITVSTPGNYTVKVTNSNGCSNTSS
                     AMTVTVNSSFQPTITVSGSLSFCSGGSVKLTATKGSSYLWSTGATTRKITVTSAGNYA
                     VTVTQFTTGYVGTSSTVNVNVLPAPAATITANGSTTICQGSSVTLTSSNGANYLWSNG
                     SISNSITATTAGNYIVTVTGSNGCTKTSAATTVVVNNCGSSCPTPGNLHVTNLRPSSA
                     EMNWNLTSANNYYFKVENLNTGYVYYSNALSSGVNSITIGTSPSTPYRWAVRALCGST
                     YSTWSAYNQFTTPAARMGEGNSNEGLNITPLTTQMPSEFEENPLRLESDDASVTFFPN
                     PATSFVVFNVTASEKNTATLTLLDLSGRTLNKENYQFNEGLNNGKIDVSNYARGVYMI
                     QLVFDGKTVMKKVVLE"
     gene            7777..8316
                     /locus_tag="PKY09_05365"
     CDS             7777..8316
                     /locus_tag="PKY09_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNB12318.1"
                     /translation="MKFRIFSIMLFTFLCFGQKAKAQESENIIEDNLRNYYSQILGYQ
                     VKEVFNTKVFDLIDTWLNTPYKYAGKNENGIDCSGFVNVIIKAVYGSSFGCSSVDMYN
                     KLPHVSLKNLNPGDLVFFKTRGRRISHVGMYLGQDKFVHSSSSSGVIVSDLNEPYYKK
                     RFAKGGRITLDESTTVVSK"
     gene            complement(8321..9955)
                     /gene="pruA"
                     /locus_tag="PKY09_05370"
     CDS             complement(8321..9955)
                     /gene="pruA"
                     /locus_tag="PKY09_05370"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNB12319.1"
                     /translation="MTNAVYNIPPAYNEPVLNYAQGSPEKTALKAALAEARSKQMDIP
                     MYIDGQEVRTGNTSALRPPHDHTHVLGMYHKGEKKHVQLAIDAALKAKKQWAEMPWEQ
                     RAAIFLKAADLLAGPYRYKMNAATMLGQSKNAYQAEIDSACELIDFLRFNVQYMREIY
                     TQQPPVVGKGVWNRTEQRPLEGFVFALTPFNFTAISGNLPTAPAMMGNTVVWKPANTQ
                     IYAANVVMQLLIEAGLPAGVINLVYVSGPDAGDVIFKHPDFGGIHFTGSTEVFKGIWK
                     TIGENMPLYKSYPRIVGETGGKDFIIAHKSADIDVLVTALARGAFEYQGQKCSAASRC
                     YIPKSIFEEVKSKLVKEVKSMQLGPVEDFRNFVNAVIDEKSFDKISAYIDNAKKSKNA
                     EIIVGGSFDKSKGYFIEPTIILTTDPKYTTMCEEIFGPVLTMYVYEDEKFEETLHLVD
                     STSEYALTGAIISQDRYAVDVATRALVNSAGNFYINDKCTGAVVGQQPFGGARGSGTN
                     DKAGAMVNMLRWVSPRTIKETFVPAKDYRYPFMESE"
     gene            complement(10044..13838)
                     /locus_tag="PKY09_05375"
     CDS             complement(10044..13838)
                     /locus_tag="PKY09_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB12320.1"
                     /translation="MASTGNKQNTDNLTTESEVLYNRILFAVIALCYLLFGIFFSEKV
                     IDVLLPLTWMALVPLALLAVSFFLPVAKKYSGEQLSGMFLLLTIHLMFFLFRNKFSGH
                     PEFLLVTVILFSNLHIRNVIYLLLYNVIVLGSLEYILISGTSGSSDPVRLFFIIVIAM
                     VLSFLYQWFNLRRQGMNKDGEKSLENNLEKLPFAAMIFDRDNLGLLYCNREAQNLLGI
                     DSQAEMDIRTVFKLDDFSDTELLAKSKAGNFNNIVVETFQQLKVLLTIKQSEEELLVF
                     AEREKIKEKESVSLPGQYFVTTTDGKIIDLSKEAEDFITVDLGVLQHLFQNFIIENKP
                     LHNFSVLKINEQDPFALQMQTGTLDNIQVYYWTPIRNLRPVESINAEVVLPDEGWSQL
                     VIDSGNELISFSPSFINTIGYDEMELGRIPVIQLIHPADTLRWQQLIAECRKKERVNA
                     TVRFIHKDGKVHFLKSFFSSNDKDEVKIYAEDITESILLQQELTNARANVSAVIENTN
                     DLILSTDMNHLITVINHSCQHYLNTLTGKMLNVGDDFRAAMKEEDQRLWSQRHTEVMR
                     GKRVTYNDEVSKNGTERYFEFSLQPVVDEHNIVAGVSLFARDVTQRVHFENELVKAKE
                     TAETATAAKSQFLATMSHEIRTPLNGIVGMLELLRMTTLNDKQREYVSTLQLSSENML
                     SIINDVLDFSKIESDKMELEYEPFELKKVIEETFDLLYYRALGKRLELFYNIESTIPA
                     YVMGDSMRLKQVLVNLVGNAIKFTEKGHILITAQLIPSVSEKLNIKFSVKDTGAGISE
                     EQRQRLFQSFQQADTSTYRKFGGTGLGLTISAKLVALMGGTIELMSEPGAGSEFYFTV
                     ITQPAPIAAARNVRSNLRILRGKKILIFSTSTDFDLHLSDLFNDWHILHQSVTQLEQA
                     KKELTPEANYDALILDAQMQDYLLFAEELKEQIRSSNIPVFAFNARFSGGDIIYGNKL
                     FEAVLPAGVDAIKISSVLTKSFIDSATSFARGDDAVPVFDSQVALKYPLQILIAEDNP
                     INQTLAVTVLEKLGYKPDVVENGKLVLEKVTTRKYDLIFMDVQMPEMDGMEATAALRK
                     LNNSSIIIAMTAFALEDDKKQCLDAGMNDYTTKPIRIEVVQSLLEKWGEVILKSKASR
                     NDEPLIDKQTIERLKAMAVGDDTSFVKNIFELFLKQMDNLHADMSNFFSEDNSDGLYK
                     ASHKLKGSALNIGAKRLSEVCRIIEEKGKLADLTGMNELLQKLAEVISLTKNEIRTLS
                     LA"
     gene            13954..15027
                     /locus_tag="PKY09_05380"
     CDS             13954..15027
                     /locus_tag="PKY09_05380"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNB12321.1"
                     /translation="MAEIKKVLGLMSGTSLDGLDLALVNITEANDGMHYEIAAAETIE
                     YDQKQRDELTNAKMLSGERLFELHHKFGVFLGESSLKFLQKHQLSCDLIASHGHTVFH
                     QPANGFTTQIGHGAAIAAVTGITTVCDFRSIDVALGGQGAPLVPIGDELFFGMYDYCL
                     NLGGIANISFRKNLNRIAFDICPVNMALNYLANACGQNYDAEGKIAASGKLNTALLDK
                     LNALEFYRTPYPRSLGVEWFNEYFLPVLNASTASLTDKTTTVTHHIAKQIGDSLTISD
                     NKLKSTLLVTGGGSKNKFLVQKIQENTSVQIIIPDEKIIDFKEALIFALLGYLRINNH
                     KNTLATVTGAKKDSVGGAVYLMA"
     gene            complement(15064..15246)
                     /locus_tag="PKY09_05385"
     CDS             complement(15064..15246)
                     /locus_tag="PKY09_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12322.1"
                     /translation="MRNQRVEQKKSIKDNWETDNYHAKKHKMSNHREEKNWKNKILKE
                     EEEVPDEDFFRFDDEE"
     gene            15381..16607
                     /locus_tag="PKY09_05390"
     CDS             15381..16607
                     /locus_tag="PKY09_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNB12323.1"
                     /translation="MLDLLKKFEEKRPEIVFEWSDAETGAEGWLVINSLRNGSAGGGT
                     RMRKGCDKREVESLAKTMEVKFTVSGPPIGGAKSGINFDPADPRKNQVLERWFKAIKP
                     LLKSYYGTGGDLNIDEIHDVIPICNKLGILHPQEGTAVGHFNDGEENIKKRISNLQKG
                     VSLELKDSNLSPDLDNKYRVADMITGYGVAYAVKYYYELWGGKISEKRAIIQGWGNVA
                     AAAGFYLAQMGVKIVGIIDRDGGLINEKGYSFEEIKTLFNNRKGNKLHADNMLSPQQM
                     AEKVWNLDAEIFIPGAASRLVTQEQMQSLISKKLEVISCGANVPFADKEIFFGPIGMY
                     CDKHVSVIPDFIANCGMARVFAYLHNANAVIDDRLIFDDAANTIKNALQEVIATHKDK
                     KGIASAAFEIALKKLL"
     gene            16607..17710
                     /locus_tag="PKY09_05395"
     CDS             16607..17710
                     /locus_tag="PKY09_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNB12324.1"
                     /translation="MFIDVLQQRILGNTVEDYLWFAGLILAGLIFKNLISRILTFLLF
                     NVLRKYFKTIGVKKFVELTQKPFSTTLFLIFIYIACRYVHFPDEWKLASESEFGVRMT
                     IKLIFQIATVFFITWTLLRIIDYIGLVMRARANKTESKMDDLFVPFFVSSIKALTVII
                     SLFFILGVVFKLNVASIIAGLGIGGLAIALASKDTLENLLGSFLIFMDKPFVVGDLVK
                     TDNLRGHIEEVGFRSTKVRTIEKTLVIVPNKKMMDAELENQTERTFYRHRFVIGLVYS
                     TTADQIQTITQQILDILNEHPLVNENPNVFFINFGASALEIEVIYFVKTQELSDFENT
                     RQEVNFAIMKIVAANKSDFAFPSTSVYIEKEVK"
     gene            complement(17893..19536)
                     /locus_tag="PKY09_05400"
     CDS             complement(17893..19536)
                     /locus_tag="PKY09_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12325.1"
                     /translation="MKTNLFEWKGMLPFKVSIILFLGTCIQNIAQGQPTIAWQHCFGS
                     ALVEMAWDVGQTSDGGYVLLGETTVGNGGDVSGNHGNDIWVVKTDTVGILQWQKCLGG
                     SGFDRARRIKQTLDGGYIIAGTTDTPNDGDVSGYHDSLGYSDAWIVKLDASGTIQWQH
                     CYGGADVDNSWDIVQNSDGSYCFVGTTGSVDGDVSGLHGGLSSDVWLVKISNTGSLLW
                     QKCLGGTRGERGTSVIQTTDGGYILTGSTYSVDGDVSGWHVDTIAGNWWDDVWVVKTD
                     AFGNIQWQGCYGGSWQDGGNQIIQTADGGYLVAGATNSDDGDVVGFHSNFYRYYDGWA
                     FKLNATGTLQWQRCYGGTDTEGFYSAEQTADGGYILAGLVKSIDGDVVGNHYEDLWAV
                     KTDTVSNIVWQRCLGGTGSEYFGVIRVTNDKGYIGAARTYSNDGDVSGNHITTQPSSD
                     CWLVKLGTDTTVSIAAIAHTINFTVNPNPSKDYFTITGLQAQKQYQLNIVNITGVTVY
                     KETVKNQVTWMVHHRLPPGVYLITLSNEETKTIRKLIVQ"
     gene            complement(19533..20843)
                     /locus_tag="PKY09_05405"
     CDS             complement(19533..20843)
                     /locus_tag="PKY09_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12326.1"
                     /translation="MSTGEWAIAVYQDTYNDYIMAELFEWRSSTNSFNSYSTIKIYQG
                     GINVKIDGFWDSSTGDRWMLVWNKDDQLAVFATAGYYDFNLSKPVIDISKNVILQGNG
                     TSIYSAPDVTILRNNVYFTYNEFDGTNNILTVEKQNWLVIKLGQITTPTNYVLNYSNT
                     DYAYDLPRISSFFDTYYNYDNWAVVAQTINATTGGTEIHGFATSWYHPLNSTPYVYND
                     GSLASPFNNNDITIYQNSYPVISYVGDYIFILWQVDPIGYAVSVPDQQDIIGIRMNMD
                     GQTFSYQSEYYFVVNRSTKKNQLAPSISGRRALQFSNFLAAFYNDDTGAPGGQDVYFK
                     QNIYSASFNTLFRVAATENQSPSEVFPNPFNDKVVLNLEGSELDIYSISIIDGKGKVI
                     EKIENVQKKEYIWSPSKEMASGLYLLQITNTTTGKTQTQKINKL"
     gene            complement(20830..21150)
                     /locus_tag="PKY09_05410"
     CDS             complement(20830..21150)
                     /locus_tag="PKY09_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12327.1"
                     /translation="MKKSNLIASLMATITFVLLVQWHQSYGQSFYPANNADVSSRCYV
                     SGTVNTNNDYIESPLGSTKRSVIVYDGAHPGLFWDDGNGNTGCTLLGAGIPNCIVVNP
                     DVNR"
     gene            complement(21219..21443)
                     /locus_tag="PKY09_05415"
     CDS             complement(21219..21443)
                     /locus_tag="PKY09_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12328.1"
                     /translation="MKTIQYKVALIEVMHESIQLIQPSSQDKFWQAELKHFIFLSSSH
                     FLGGVVLLFIPETLFLKRAVKPYPLQIRYF"
     gene            complement(21615..22127)
                     /locus_tag="PKY09_05420"
     CDS             complement(21615..22127)
                     /locus_tag="PKY09_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12329.1"
                     /translation="MAGLKGETKLNVVYDYSNMGVGSFAKEEEYVAKKTREYNANEPG
                     RGDSWAKSWVADRKNRFEPSFVELFNKKSPILIGEFPDAKYTMKVSTTFTEPGFNVYV
                     TRKNASINLEITVYETANPDKPICKMQGLRFPGRTFGGYDYDTGTRLSESYAKAGKDL
                     AAFIGKNLKK"
     gene            complement(22356..23303)
                     /locus_tag="PKY09_05425"
     CDS             complement(22356..23303)
                     /locus_tag="PKY09_05425"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNB12330.1"
                     /translation="MQALSSTNFIFQGQTAFYKGKVRDVYSIDNKWLVMVVTDRVSAF
                     DVVLPRPIPYKGAVLNQIAAHFMQATADLVPNWIVNTPDVQVSIGIPCQPLKVEMVIR
                     GYLSGHAWREYKQGKRMLCGVKLPDGLNESDVLPEPIITPTTKAAHGHDQDISKEEII
                     KQGIVDAAIYEQAETYTKQLFRRGSEMAAQRGLILVDTKYEFGVHQGKVYLIDEIHTP
                     DSSRYFYAEGYAERQKSKQPQKQLSKEFLRQWLIENNFQGLAGQTVPEMSDEIVQMVS
                     DRYIELFENITGKSFVKPADENIEQRIYTNIVKALEKLF"
     gene            23368..23931
                     /locus_tag="PKY09_05430"
     CDS             23368..23931
                     /locus_tag="PKY09_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB12331.1"
                     /translation="MRLFEEKTHLPNIKWMSGQEKISGKQKAFTDAYQQLHEKLVRFV
                     QSMVYNREDVKDIVAETMLVAYEKFETIRHKDALLSYLFTVSSRLVYKLQDKKKQIYT
                     AIDPEQAEQVSDEHTGADSKVQLKELYNALDQLPLKQKEAIVLFEISGLSLAEIQQIQ
                     GDSLSAVKSRIARARESLKKILEEEIV"
     gene            23953..24774
                     /locus_tag="PKY09_05435"
     CDS             23953..24774
                     /locus_tag="PKY09_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12332.1"
                     /translation="MENNKLNKLFDAARNADPVVSLDESLEQFNKEVEGKETTSVYLK
                     WLKPVIAVATIAVVVGGIYFLVQNQDQQNAQQSPENTAQTAEPTLQATETQPDADIEQ
                     PQSKDENIEAGTSSTKTEITNPAPIKNKVTHAVGANDAYIVETQTVNINNENGAFKIS
                     ISGDNVERIVLNGKEVEKSNWSNYQDEIAEAMAAKSTLGINKENAANKKFVDYMFTTL
                     KENGLIKNNLAVIKLSNDMLLIEGQEADSAIHQQLLKEYKKITGKDIGLRTLYFN"
     gene            complement(24767..25897)
                     /locus_tag="PKY09_05440"
     CDS             complement(24767..25897)
                     /locus_tag="PKY09_05440"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNB12333.1"
                     /translation="MNTFNPNIVIGVLGGGQLGKMLLQKAADYNLSIHVLDPDESAPC
                     RYMCKTFEHGDFRDYQAVMAFGNDCDIITIEIEHVNIEALYELEKLGKRVYPQPHILE
                     MVQDKGLQKEFYQKNNLPTAPFYLVDKPEQIKPEFPFVQKLRKGGYDGKGVLVVKDAD
                     GMSKMFQAPSVIESKVAIAAEIAVITARNNSGEIKSYPVVEMQFNAEANLVELLFSPS
                     KTSASIQHKAISIAEDLINKLNMVGLLAVEMFVTKEGQVLINEIAPRPHNSGHHTIEA
                     NHTSQFEQHLRAILNLPLGNTASVLPAAMINLLGDKDSNGKAIYSGVQEALNAGDVHI
                     HLYGKKITKPFRKMGHVTVTAATVDEAIVKAQKIQSQLKVIS"
     gene            26024..26560
                     /gene="hpt"
                     /locus_tag="PKY09_05445"
     CDS             26024..26560
                     /gene="hpt"
                     /locus_tag="PKY09_05445"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNB12334.1"
                     /translation="MNEVTILDKTFTIMIPRKKIETRILEIARDLNSEYKDKNPVFIG
                     VLNGAFLFMADLFRNIETPCEISFIRVSSYSGTESTGQMKNVVGLSGNINNRHVIIVE
                     DIVDTGETVSYLMQEISKHQPASVKLATLLFKPEALKKPVTVDYAGFEIPPAFVVGYG
                     LDYDGYGRNLQDIYVLKS"
     gene            26617..27186
                     /locus_tag="PKY09_05450"
     CDS             26617..27186
                     /locus_tag="PKY09_05450"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNB12335.1"
                     /translation="MLNIVLFGPPGAGKGTQSEKLIQHYGLIHLSTGDLLRNEIAGQT
                     KLGLEAKKLMDQGVLVPDEVVIGMIESKLDANPTAKGFIFDGFPRTTAQAAALDNLLL
                     QKNTTIKMMLALEVTEVELSKRLLLRGKDSGRPDDQNPEVIKKRINEYNNKTAPVADY
                     YRKQNKYYGINGIGSIDEIFNLLCKAIDK"
     gene            27212..27868
                     /gene="fsa"
                     /locus_tag="PKY09_05455"
     CDS             27212..27868
                     /gene="fsa"
                     /locus_tag="PKY09_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNB12336.1"
                     /translation="MKFFIDTANLAQIKEANDLGVLDGVTTNPSLMAKEGIKGKAAVM
                     KHYVDICNIVKGDISAEVIATDYDGIVAEGEELAKLHEQIVVKVPMIKEGIKAIHYFA
                     SKGIKTNCTLVFSAGQALLAAKAGATYVSPFIGRLDDVSTDGLQLISEIRQIYDNYGF
                     QTQILAASIRHAMHIINCAKLGADVATCPLNAITDLLKHPLTDIGLEKFLADAKKFAN
                     "
     gene            27880..29547
                     /locus_tag="PKY09_05460"
     CDS             27880..29547
                     /locus_tag="PKY09_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12337.1"
                     /translation="MKKNKEVVAKKSNTKTANKSSSETTLVKLENFLLKNETIIFFSI
                     LLVSLIFSVLLFDIKMSEGNDDSDYIEGAFKLTKDFSSYYSTKAPLYPIFLSLPVSIW
                     GINLPILKSLSILFSLLNLTAMYYAFRKLIPYSILFAVLIFTGINSYFLYFASQTYSE
                     AFFCFLQALLFITIRPILIASKEDNIPIAKYLVFGITLTLLSLTKNITVGAIAAVALY
                     FVFNRDFKKLAYAFGSFVVVRGIFEVIKSFIWGSQNQFSNQYQILIQKDPYNASKGVD
                     DFGGFVDRLFSNTSIYIGKRLYQILGFVSPENTKTNSGLVLITFLFVGIGLYFIFKNK
                     ARIMQFTFLYVLLMAGLTFFVLQTSWDQPRMVLVYVPFIMMIIFYGLYYAFQNTPALR
                     SFVFLIVSIIIIMSSLIKTADKSVANLPILKKNMGGDIYYGYTEDWTNFLKLSRYCGD
                     SLPETAYVVSRKAPMSFIYSNGKSFYPIYTVPSTDPDSVLALLKENKVTHVLIASLRR
                     NPKKNDGYVINTLHRMMGPVGQKYPDKLKLVKQVGTSEPSYLYEIKY"
     gene            29547..30278
                     /locus_tag="PKY09_05465"
     CDS             29547..30278
                     /locus_tag="PKY09_05465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB12338.1"
                     /translation="MSDRISNELEHGKKLLEGGAEAIWNWDSPAGKVRADRRADLIAQ
                     YSLADKSSTVLEIGCGTGLFSGKFYQRTGAKIIATDISPELLNVAKEKYPEVEFVLAD
                     AMQIQFDANTFDVVFGSSVLHHLEFERSLNEIFRVLKPGGRMVFAEPNMLNPQIFIQK
                     NIPFIKKWLGDSPDETAIVRWSFTKLLRQKGFTNVNIFPYDFLHPVVPGFLITTVNSI
                     GKIVEKTPLLKEIAGSVIIVAEKQR"
     gene            complement(30275..31087)
                     /locus_tag="PKY09_05470"
     CDS             complement(30275..31087)
                     /locus_tag="PKY09_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12339.1"
                     /translation="MKKVVLNLICAMLFSNFKSFSQNCELLCNGDFENPLVISTPHFV
                     NNNAMRCWQTTATDSLMELWESGYNGVTAYSGNQFMEINANQVAAVYQDVSLIAGDSL
                     TISFAHRARFGIDTMSVSLGTQNNLPTVLGYFADSTSSWGHYVINYTIPVSGYYRISF
                     NSVYSGGNLASLGNFIDAVSVCGSSVGFDDYKQTGTFFIYPNLSTKGVTIKLNDDGKG
                     VLTICDILGKTLYSATLNLTENQIETGPLERGVYVVSLLKNNKIVSKRFLVN"
     gene            31261..32073
                     /locus_tag="PKY09_05475"
     CDS             31261..32073
                     /locus_tag="PKY09_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12340.1"
                     /translation="MKPTILAIFLFCFSNISAQTYLTSGGARASALSDAAVTINDVFA
                     AFNNQACLSMIEKPEAGLALQNRFGMKELNTLSAAFALPLASNKGVFALSVNRYGYKL
                     FNQNKIGLAFAKKLSPVFSAGVQVDYLNTHIDEGYGNKSSFTIEGGVLAEFNKLKAGV
                     HIFNPLHVKITDYDDERIPVIIKAGISYSFSEKVLAAAEIFKSIDSKENFKIALEYHP
                     VKALYVRGGVSTEPVQLTFGFGLKFSQFYADFTSGYFEPLGYSPGLALRYAF"
     gene            32076..>32866
                     /locus_tag="PKY09_05480"
     CDS             32076..>32866
                     /locus_tag="PKY09_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB12341.1"
                     /translation="MKTIQLFFISFLVCNLTFAQVVPPQDDSKIEEKIENIANTADES
                     ADLTELTEQLQQLAANPINLNKATREDLLQTGLFSEVQADAIIEHREISGKFISLYEL
                     QSIDYLSLEDIEEILPYVTVGNTEDIKATLKKIFSDGNSRLIVRGQRYLEKQKGFRDS
                     TATDKVHPAYPGSPWKLYTQYQYRYKQRFSFNLTAEKDQGEEFFKGSQKNGFDYYSAH
                     LAIRDFGIIKRAVIGDYDLFYGQGLTLCTGLSFGKSPDVAAVRKFS"
BASE COUNT        10400 a         6315 c         5848 g        10303 t
ORIGIN      
        1 tggctgaatg agcttctatt ttattttcat gctgtttaaa atcatgtcgc atgatatcca
       61 gaatatctct ttgcaggata aactgattct ggtaatgctc cacattgggc atgttgtaat
      121 gatttctatc agtcataact gcagaggcaa gatcggtttt catccggtca atctctgcac
      181 gatagaagtt aattttacct tgccactctt tgtgctcggc aagcatttct tcaagttgtt
      241 cgatggtgtg catgttatta agatttagag tgaacgaaat aattgcggtt tatctataca
      301 acacgcttct ttggaatgag aagccacaag aaagaaaaat atcttgcctg aaaagaataa
      361 tgatgaaaaa taaatactgt aactataatt tttcattctc aaacagacta aatttttcct
      421 aaaagtaata ctttttatca ttaaacaagc atattgttca aaaactcctg aaaacgttca
      481 cacattcatg tgttttttag ggttatcaac aaccttcaac cctaccttat cagaaagaca
      541 tgttttttat atgcttttca atgcttattc gctctgataa tggctgctaa agccatattt
      601 ctttcacctg aaaaactcag gtctttctgt ttcgggtatt ttctttcaga ggagaattca
      661 caaaacaatg caaaatcctt tactcaggat aataaaatct tacagatgtg atatcgctgc
      721 gcttttcaaa caattaaacc acattcggga aagcaatttg caattcactg tacaaataat
      781 aaataatatg aaaagaattg cagaaattgc cttaatgtct atgtgtgtcc ttagtgtgtg
      841 taaatgcaca tttgtaaatg cacaaagtaa cattgcagag cgcaagtaca gggtcattgc
      901 ttacaagcag ggagccaatg aaatcaccag tatgtcgaat gaaactacgg tgacacctaa
      961 tatggctatt tatgtgccca actcctttac tcctaatggc gatggtataa acgaaacatt
     1021 tggagcctac agcgaaggtg taaaagactt taccatgcag gtttatgatc gctggggagc
     1081 tatgattttt gaatccagtg atatcaataa gcgctgggac ggtatgtata aaggtcagat
     1141 agcacagcag ggaagttatg tatataaaat acaggcaaaa gctatcaacg gcagaatatt
     1201 gactaaaaaa ggaaccgtaa accttataat gtaatcgcga aaaaaagata ataatgtttc
     1261 acactaaaaa ctaagaacat tgtcaatcat actactaaaa tatctcatca ggcacttcga
     1321 caaaaaggga atagtagtta cgcaaataca taagctgaaa actaaaattg ccatgcttgg
     1381 tttgtgattc ctgtaaagtt gctactttag ggcaaacttt cgggctacat gaagcacatc
     1441 gcaaaattat tatggcttac ttttcttatt cctcttttat cgtcaactgc ttcggcacag
     1501 gattctgaag aagaaacatc acgctggcaa ataggattaa cagcaggcat taacattcca
     1561 ctgagtgatt ttgctgataa gctggacgaa aactttaaat ctggctatgc catacttggt
     1621 gctacctctc gactgagagc ttcttaccac ccaaacagca aactctatat tgctgccgat
     1681 tatgtgtggt tctataaccc ggttaatgca actgaattac taaacagtct tgttgaaaca
     1741 aaccctccgg gaccacgtta caaaaccact acttatccgt ggaaagtaaa cagcctactt
     1801 ttaggtcttg gtacaacctt aaattttaac gaacaaacag atgctacatt aagaggaatg
     1861 cttggttttg caaatggaaa atattcaaaa cggatttatc agtcttttga tggcgtaaaa
     1921 acattgcagg ttacagaaaa tgcttcatct gttttagata tagcactcaa cttaggcttt
     1981 gaagtgaaat attgttttca caaaaacata ggagtaatgt ttgcaggtga ctatttccgc
     2041 actgaattta actatgatga tgtgtattta atttattccg aaaccggaga aaaaagatcc
     2101 accggtgcat actcacaacc tgttgatgtt tttcagtttt cagccggtat ttttaccaaa
     2161 ttttaatggg cgaaaggttg atttttttgg gtgaaagtcc tttttttaat ggcgaatata
     2221 acgttattaa cacctgttaa taacgttata tattgattat cattgactta tagtataatt
     2281 gccaatcacc tcttttttgg gtgaaaattc aatttatttt tggacgcgtt aaccaattat
     2341 tctattccgt ttaacaacaa aatttattaa tgcgaaaaaa cttcggttaa tcgcagcggg
     2401 cttattatgt ttaatttcta attaccattt cacatgatta aatcaacttt aatctcaatt
     2461 acaagaagca ttgttgcagc gttcttgtta gcagttccta cttgtttggt tgctcaaaat
     2521 tatctgccaa aatcgtatta tactttcgaa ggctcatcgc caatgaaaga ttcgatggga
     2581 acttttaatc ttgatccaaa ctattaccag tcaaactatc aaatactaac caacacatcc
     2641 ggtggtgttg gcaagtacat gaagttagac tctagcagta ccatcatcag aggtggtgtg
     2701 ttggctattg actctgccgt aacattcgaa tttattttta aacccggtat gaactttggc
     2761 acaacagtat ttgcaaaaag aatggatggt gctcttgaat tgcgcatgac ctatccggca
     2821 attaacttca tgacagaagt taaagccgct aacggctctc ctatttatga tgattttgaa
     2881 atagacctta atggtattgg tcgtgcatct tatagttatt atgtggatga caattggcat
     2941 cacattgtat ttaaatataa tgcaaaagcc ggcatcaaag aagtctgggt tgacgggcaa
     3001 ctgcctgctg gtttttcaaa aactatagcc ggtggtagtt tcgcccaaag tggtaataga
     3061 gactatatgc tcaactctca ggcagattac tataaatatc agggtgcaat agatgaaatt
     3121 gctgtttact acaactcact tggaggaaat tcaatctatg cacactattt aaattttatg
     3181 gcaggcaagc attattcatt cgcaaaccct acggttcctg caccaacacc aagtcccgta
     3241 acagcaggta tcaatattaa cgactttgct ccgggtcatc cctcaccatc acttacaccc
     3301 atagagcaat taaaaaactt tcctacacca agatataagc ccgggcatac attgtttcct
     3361 aacaatccat ttgttggcta ctattatctt tcaggatatc agacttatga gactgtttat
     3421 tcaaaagctg tagcaaacag tgttacacta caaacagatt ttgcaaaaaa ctttaactac
     3481 accattatgg ctgccgaaaa tacaggtgcc tatagtgatt actcgaaccc aactacattt
     3541 tcatatgcat ggattaatct ggcaaacagc tatccacaat acaaagtatc tgctttatca
     3601 tattggccac aattgcgtcc gacagatgca ggctataaaa gcaatactgc ttatgcaaca
     3661 tgtggatgtt tgcccgataa gtattatctt aaaaacagtt ctaatcaata tctgaaccct
     3721 aacggaggaa ccggttcaac aaaatatctg agtcctgcag gagcacctga ttctttagtt
     3781 aaagatggac ttacacagaa attttacatg caacaactac ttacaaaatt gagcagacct
     3841 attgattata tattagataa tggtgaagtt attccattcc tatattatac taacgttatg
     3901 caatctgacc cgagtgtggt ggcagataaa aattcatcag gtcttgattg gttcacatat
     3961 gaaggtcgca gagctgcaac aaaaataaaa gcttaccgtg atcagttcat gggattaagc
     4021 ggtttatcaa atacaaaatt tgcgtattat cagttggatg gtcaacctac atggaattta
     4081 aaatggtctg aagttcgtac tgtaaacagt gccatgaatg gctcaaatta tgcaaccggt
     4141 gatatttata tgcgttggcc atcaaactgg cgttattgga gtggcgcatg gaaaggatgg
     4201 caatgggtag ttgagtccag aagagaacaa cttttggctg gcgacaaata tttttcacct
     4261 gcggtttcac cgggatggga caatgatgaa acacaaaacg ttcgtcctgc acaatggtta
     4321 ggtttcctca aggccgtttc tatgacaggt gctgaatttt tcactacagg ctattttgta
     4381 acatcgcaac cctatcagaa tccaaaaaat tatatctggc aattggttat tccaccttat
     4441 gcacaggcaa tcacaagccg ctatgaagac ttgtttagaa atggatttct tatgtcaggt
     4501 gatgtgccta acagtacaac cagtccaacc tatatggctt actcatttaa tacaggtgac
     4561 cctggaaaat tagttgttgt acgtaaacac aattcacaga ataaatatgc cataacaggt
     4621 tcatcacaac caatgtctaa tatggctggc gctgcacctg atgaagctgt tgcacaaatt
     4681 aatttagatg gcagtagaat taagtttaaa atcagaagac aaggtagtac atatatttat
     4741 gataacacca acacttctgc accaatattc tatcagttag atggatggca tgaaaacacc
     4801 tatccatatt actggtcaaa agattttaaa tttgaagcag agctttatga caacagcaac
     4861 aataatgtgt atctgaaaac cacccgtcct tcaggagcag cggctggcga ctttacagat
     4921 tacactactg ctgtaggttt taattctgtt agtgatttaa catttaattt ccagccaaga
     4981 tccacaacca attacactta ctacgtttgg gtgagagcac gttctaaaaa ttcacaaact
     5041 acaggaatga ccatttcttt agatggaagc aacagcaaac aaataggttg tattactgat
     5101 gcaaactggc agtggtataa atatgatgta aactcaggcg ctgccatttc attttcatct
     5161 gtaacatcat tagctgatca cattttgaaa atcactccaa caaataccaa tcttgagata
     5221 gatcagattc atttatctac cagcagcaat gctatttata caagccctgc cccatgttca
     5281 ggtgttgcag tgccaacaat tactgcaagt ggacctgttg cattttgtgc aggaggaaat
     5341 gtaaatctta ttgcaagcac tggtctctcc tatctgtgga gcaatggttc aacagatcct
     5401 tatatcaatg taactagtgc tggttcttat gttgttactg ttaccacagc agcaggaact
     5461 gcaacatcat caccggtaac agtaacggtt tattcgctgc cgaatgcaac aataactgca
     5521 aatggctcaa cttctttttg tgttggtggc agtgttacac tcactgcaag cagtggctca
     5581 acttatcttt ggagcagtgg tgcaacaaca cagtctatta ctgctattgg aggaggaaat
     5641 tataatgtta tcgtaacaaa ttctaatgga tgttctgcaa tgtctgcttc aacagcggta
     5701 acagttaaac ccgttcctac tgctaccatt tcgcctagtg gtccaaccac ttttgcttcc
     5761 ggaggtagtg ttacattaac atcttcttca gcaggtacag gtggaagtta caaatggtca
     5821 ccaggtggta tgacaactca agcagttaca gtcagcacaa caggaaatta tgttgtgacc
     5881 gttaccaaca acagtggatg ttcagcaaca tcagctccac tatctgttac ggttacctca
     5941 ggagcaccaa cacccaaaat aacagcaaat ggccctacaa cattttgtca gggcggaagc
     6001 gtggttttaa cttgtagtgc agccaattca tacttatgga gtaatggtgc aactacacaa
     6061 tcaattacag ttaccactgc aggcagttat acttgtactg caaccacagc aggtggttca
     6121 gccactgcag cagcagtgaa tgtgattgta acaacaccta cacaggcaac tattacagca
     6181 tcaggtagta catcattctg ttcgggaggt agtgtgacac ttaatgccag ttcaggaact
     6241 tcttacctgt ggagtacagg tgcaactact aattcaatta ctacatcatc tccaggtaca
     6301 tatactgtaa ctataactaa caatggctgt cagtcaacaa ccaataaagt agttactgtt
     6361 tatgcattac caactgcaac aataacagca aatggttcaa caaacctaca gcaaggtgga
     6421 agcgttcagc taacctcttc aagtgccgga agtggtggaa gctatttatg gagtaccggt
     6481 gcaactacac aatcaatcac tgtttcaaca ccgggaaatt atacggttaa agtaacaaac
     6541 agcaatggtt gtagtaatac ctcatcagca atgacagtaa ctgtaaattc atctttccag
     6601 cctaccatta ctgtcagtgg atcattaagc ttttgctctg gtggcagtgt taaactaaca
     6661 gcaacaaaag gttcatcata tttatggagt actggcgcga caacacgtaa aattactgta
     6721 acatcagcag gcaattatgc cgtaacagta acacagttta ctacaggcta tgtgggaacc
     6781 tcttcaactg taaatgtaaa tgtattacca gcaccagcag ctacaatcac agctaatgga
     6841 agtacaacta tttgtcaggg ttcaagtgtt acattaacat caagcaatgg tgctaattat
     6901 ctttggagca acggttctat atcaaattca attacagcaa caactgccgg taattatatt
     6961 gttacagtaa ccggaagtaa tggctgtact aagacatcag cagcaactac agttgtagtt
     7021 aacaattgtg gctcatcttg cccaacacct ggaaatttac atgtgacaaa cctcagacca
     7081 tcatctgcag agatgaattg gaatctgact tcagctaata actattattt taaagttgaa
     7141 aatttaaata caggatatgt ttattattct aatgcattat caagtggtgt aaattcaatt
     7201 acaattggaa cctcaccttc aactccttat cgttgggcag tacgtgcatt gtgtggcagc
     7261 acatattcaa catggagtgc atacaatcag tttacaactc ctgctgcaag aatgggtgaa
     7321 ggcaacagta atgaaggttt aaatattact ccattaacta cgcaaatgcc atcagagttt
     7381 gaagaaaatc cacttcgcct tgaatcggat gacgcaagcg ttactttctt cccaaatcct
     7441 gcaacttcat ttgtagtgtt taatgtaact gcatctgaaa agaacacagc aacattaacg
     7501 cttttagact tgtcgggtcg tacactaaac aaagaaaact atcaatttaa cgaaggactt
     7561 aacaacggca aaattgatgt cagcaattat gcacgaggcg tttatatgat tcaattagtt
     7621 tttgatggaa aaactgtgat gaaaaaagtt gttcttgaat aattggtttt tattatcata
     7681 agtattaaga gctgtcatca gattgatgac ggctcttttt cattttgtac aatacctgat
     7741 aaatgaatac gtttattaaa atgttatctt cgcccaatga aatttcgcat attcagcata
     7801 atgcttttta catttttatg ttttggacaa aaagccaaag cacaagaatc tgaaaatatt
     7861 attgaagaca acctgcgtaa ttattacagt cagattttgg gttatcaggt taaggaagta
     7921 tttaacacta aagtttttga cctgatagat acttggctga atacacctta taaatatgcc
     7981 ggtaaaaacg aaaatggaat tgattgttcc ggatttgtta atgttatcat caaggctgtg
     8041 tatggctctt catttggttg cagttctgtt gacatgtata acaaactacc acatgtgtca
     8101 cttaaaaatt taaatccggg tgacttggta ttttttaaaa cccgtgggcg aagaatttcg
     8161 catgttggta tgtatttagg tcaggataaa tttgttcact ccagctcatc aagtggtgta
     8221 attgtaagtg accttaatga gccttactat aaaaaaagat tcgccaaagg aggaagaata
     8281 acccttgacg aatctactac tgtcgtttct aaataaattt ttattccgat tccataaaag
     8341 gataacggta atcttttgcc ggaacaaaag tttcctttat tgttcgcggt gatacccaac
     8401 gtaacatatt taccatagca cctgccttgt cattagttcc cgaacctctg gcaccaccaa
     8461 aaggctgctg cccaacaact gcaccggtac atttatcatt tatatagaag ttacctgccg
     8521 aatttaccaa tgctcgtgtt gcaacgtcaa ctgcatatcg gtcttgagaa ataattgctc
     8581 ctgtaagtgc atactcactg gtgctgtcaa ctaaatgcaa tgtctcttca aacttctcat
     8641 cctcgtaaac atacattgtc aataccgggc cgaaaatttc ttcacacatg gtagtatatt
     8701 ttggatcagt tgtaagaata atggttggct caataaaata tcctttactt ttatcaaagc
     8761 ttccacctac aataatctct gcattcttac tcttctttgc attatcaatg taagcagaaa
     8821 ttttatcaaa agatttttcg tctataacgg catttacgaa gttgcggaaa tcttcaaccg
     8881 gtcccaattg catggacttt acctctttaa ccagcttact ttttacttct tcaaaaattg
     8941 attttggaat ataacaacgg gatgcggcac tgcatttctg accttgatat tcaaaagccc
     9001 cacgtgccaa agcggtcaca agcacatcaa tatctgcact cttgtgagct attataaaat
     9061 cttttcctcc tgtttcgcca acaatacgag gataagactt atacaaaggc atattttctc
     9121 cgatagtttt ccagatcccc ttaaacactt ctgttgaccc ggtaaagtga ataccaccaa
     9181 aatcaggatg tttaaaaata acatctcctg catcagggcc ggaaacataa acaagattga
     9241 ttacacctgc aggtaaacct gcttcaatca gcaactgcat taccacatta gctgcataga
     9301 tttgtgtatt ggcaggtttc caaacaacag tattacccat catggcaggt gctgtaggca
     9361 aatttcctga aattgctgta aaattgaatg gtgttaaagc aaaaacaaat ccttcaagcg
     9421 gacgttgttc tgtgcgattc catacaccct tacctacaac cggtggttgt tgtgtatata
     9481 tctcacgcat atactgtacg ttgaaacgta agaagtcaat caactcacat gctgaatcaa
     9541 tttctgcctg ataagcattt tttgactgtc caagcatagt cgcagcattc atcttatagc
     9601 gataaggacc tgcaagcaag tcagctgctt tcagaaaaat ggctgcacgc tgctcccaag
     9661 gcatttctgc ccattgcttt tttgctttta atgctgcatc tattgctaac tgtacatgct
     9721 tcttttctcc tttatgatac atgcccaaaa catgcgtatg gtcgtgtgga gggcgaaggg
     9781 ctgaagtgtt tcctgtgcgc acctcttgtc catcaatata catggggata tccatttgtt
     9841 tggagcgtgc ttctgcaagt gctgctttta atgctgtttt ctccggactt ccttgtgcat
     9901 aattcaatac cggctcatta taggccggag gaatattata aactgcgttt gtcatacctt
     9961 aattaattta ataatgtaca aaggtacgaa aatgaaataa gggcttttgt atccttggtt
    10021 acatcaacaa aaagatttaa aaatcaagct aaacttaaag tcctgatttc attttttgtt
    10081 aatgaaataa cttctgccaa cttctgaagt aattcattca ttccggttag gtctgcaagc
    10141 tttccttttt cctcaataat acgacaaact tcagagagtc ttttagcacc aatattgagg
    10201 gcgcttccct ttaatttatg cgaagcttta tacagtccat cggaattatc ttcactgaaa
    10261 aaattactca tatcagcatg taggttatcc atctgtttaa gaaaaagttc aaatatattt
    10321 tttacaaaag aggtatcatc tcctacggcc attgccttca gtctttcaat ggtttgcttg
    10381 tctattaaag gctcatcatt tcgcgatgcc ttacttttta aaatcacctc gccccatttt
    10441 tccagcaatg actgcaccac ttcaattctt attggcttgg tagtgtagtc gttcatacct
    10501 gcatcaagac attgtttttt atcatcttcc aatgcaaaag ctgtcatggc aataataata
    10561 ctgctgttgt ttaacttacg taaagccgct gttgcttcca tgccatccat ttcaggcatt
    10621 tgaacatcca taaaaataag atcatatttc ctggtggtaa ctttctcaag cactaattta
    10681 ccgttctcta caacatctgg tttgtagccc aatttttcca gcactgtaac tgcaagcgtt
    10741 tgattgattg gattgtcttc agcaatcaga atttgtagtg gatactttaa cgctacctgg
    10801 ctatcaaaca ccggaacagc atcatcacct cgggcgaatg atgttgcact gtcaataaat
    10861 gacttggtta atactgaaga aatttttatt gcatcaactc ctgcaggcaa cactgcttca
    10921 aaaagtttat ttccataaat gatatcgcca ccggaaaatc ttgcattaaa agcaaataca
    10981 ggaatatttg acgaccgtat ctgctctttc agttcttctg caaaaaggag ataatcctgc
    11041 atctgtgcat caagtatcaa agcatcgtaa tttgcttcag gtgtaagttc tttttttgcc
    11101 tgttcaagct gtgtcaccga ctgatgtaat atatgccagt cattaaacaa atcactcaga
    11161 tgtaaatcaa aatcagtgct ggttgaaaaa attaaaatct ttttacctct taaaatcctg
    11221 aggttagagc gaacattacg tgcagcagca attggtgcag gttgcgttat aactgtaaaa
    11281 taaaattctg aacctgcacc cggttcactc atcaattcaa tggtgccacc catcaaagca
    11341 actaacttgg cactgatggt gagaccaagt ccggtaccgc caaactttct gtaggttgaa
    11401 gtatctgcct gttggaatga ttgaaacaat ctctgtcttt gttcttcgct aatgccagca
    11461 ccggtgtctt ttacagaaaa cttgatgttt aacttttctg acaccgatgg aattaattgc
    11521 gcagtaatga gaatgtgtcc cttctctgta aacttaatgg cattaccaac aagattgaca
    11581 agtacttgtt tcaatcgcat gctgtcaccc atcacatagg caggtatagt gctttcaata
    11641 ttataaaaca actcaagtct tttaccaagt gcgcgataat agagcaagtc aaacgtctct
    11701 tcaataactt tcttcagttc aaatggctca tactcgagct ccatcttatc tgactcaatc
    11761 ttagaaaagt cgagaacatc attaataata ctaagcatat tctcactgct cagttgtaag
    11821 gtactcacat actctctttg tttatcatta agtgttgtca tgcgtaacaa ctccagcatt
    11881 ccaacaatac cattaagagg tgtgcggatt tcatgactca tggtggcaag aaactgagat
    11941 ttggcagcag tggcagtctc agcagtttct tttgctttca ccagttcatt ttcaaaatga
    12001 actctttgtg ttacatcacg agcaaacaaa gacactcctg caacaatatt atgttcgtca
    12061 acaacaggtt gaagcgaaaa ttcaaaataa cgctctgtgc cgtttttgct aacttcgtca
    12121 ttataggtca ctctctttcc cctcataacc tcagtatgtc gttgtgacca caacctttga
    12181 tcttcttcct tcattgcagc acgaaaatca tcaccaacat tcagcatctt tcctgttaaa
    12241 gtatttaaat aatgctgaca agaatgattg attactgtaa tgagatgatt catgtctgtt
    12301 gacagaatca aatcatttgt attttcaata actgcactga cattggcacg ggcatttgtt
    12361 aactcttgct gcaaaagaat tgattcagta atatcttctg catagatttt tacttcatct
    12421 ttatcattac tgctaaagaa agatttaaga aaatgcactt taccatcttt atgaataaac
    12481 cttactgttg catttactct ttcttttttg cgacattcag caatcagttg ctgccaacgc
    12541 aaagtatcgg ccgggtggat gagttgaata acaggaatac ggcctagttc catttcatca
    12601 taaccaatag tattgataaa tgatggacta aaagaaatta actcatttcc tgaatctatt
    12661 accaactgac tccatccttc atcgggcaat acaacttcag cgttaataga ttcaacaggt
    12721 ctcaaatttc taattggcgt ccaataataa acctggatat tatccaaagt tcctgtctgc
    12781 atttgcaatg caaaaggatc ctgctcatta attttcagaa cagaaaaatt atgcaatggt
    12841 ttgttctcaa taataaaatt ctgaaacaaa tgctgaagta cgccaaggtc aacggtaata
    12901 aaatcttctg cttctttgct caggtcaatg attttaccat cagttgtggt aacaaaatat
    12961 tgtccgggta gtgaaacaga ttccttttct tttatctttt cgcgttcagc aaacaccaac
    13021 aactcttctt cactttgttt gatggttaac aaaaccttca attgctgaaa tgtttcaacc
    13081 actatgttat taaagtttcc tgcttttgac tttgcaagaa gttctgtatc ggaaaagtca
    13141 tctaatttaa aaacagtacg aatatccatt tcggcctgtg aatcaatacc gagtagattc
    13201 tgtgcttcgc gattgcaata caatagtccc aaattatctc tatcgaaaat cattgcagca
    13261 aaaggaagtt tctcaagatt attttccaac gatttttctc catctttatt cataccctgc
    13321 ctgcgaaggt taaaccattg ataaaggaaa cttaacacca ttgcaataac aatgataaaa
    13381 aacagtctta ccggatcgga acttcctgaa gtgcccgaaa tgagaatata ttctaatgaa
    13441 ccaagtacaa ttacattgta caataaaagg taaatgacat tacgtatatg caggttggaa
    13501 aacaaaatga ccgttactaa taaaaactcc ggatgtcctg aaaacttatt cctgaaaaga
    13561 aaaaacatca ggtgaattgt tagtagtaaa aacattccgg acagttgttc tcctgaatat
    13621 ttttttgcaa caggaagaaa aaaactgact gctaatagcg ctaaaggaac aagtgccatc
    13681 catgtcagag gcagcagcac atcaataact ttttcgctaa aaaaaatacc gaaaagcaaa
    13741 taacataatg ctataactgc aaatagtatg cggttataca acacttccga ttcagtggta
    13801 aggttgtctg tattctgctt atttccggta gaagccatca tttcttatac gcaaaaatag
    13861 gtatttggtt aaccttgaaa ttgatttttt taaagtggaa tgaattgttg caaataccca
    13921 ataatatttt gtcataatct tacatttgcc gtcatggcag aaatcaaaaa agtgctgggt
    13981 ttaatgtcgg gcacttcctt agacggatta gaccttgcat tggtcaatat caccgaagca
    14041 aatgatggca tgcattatga aatagcagct gcagagacaa ttgaatatga ccaaaagcaa
    14101 agagatgaat taacaaatgc aaaaatgctt tcgggcgaaa gactttttga gttacatcac
    14161 aaatttggtg tttttttagg agaaagctct ttgaaatttt tacaaaaaca tcaactttcc
    14221 tgcgacttga ttgcatcgca tggtcacact gtttttcatc aacctgccaa tggctttact
    14281 acacaaattg gtcatggtgc agctattgca gcagtgacag gcattactac tgtttgcgat
    14341 ttcagaagta tagatgttgc tcttggaggt cagggtgcac cattggtccc tattggtgat
    14401 gaactgtttt ttgggatgta tgattattgt ctaaacctcg ggggcattgc aaacatttcc
    14461 ttcagaaaaa acctcaaccg catagccttt gatatctgtc ctgttaatat ggcattaaat
    14521 tatttggcaa atgcatgcgg gcaaaattat gacgctgaag gaaaaattgc tgcttcagga
    14581 aaattgaata cagcactatt ggataagcta aatgcattag aattttatcg cacaccttat
    14641 ccgcgttctt taggtgtaga gtggtttaat gaatattttc tacctgtgtt aaatgcatct
    14701 actgcttcat tgactgataa aacaacaaca gtaacacatc atatagcaaa acaaattggt
    14761 gattcattaa ccatttcgga caataagtta aaatctactt tactagttac cggtggtgga
    14821 tcaaagaata aatttcttgt acaaaaaatt caggagaata catcagtaca aattattatt
    14881 cctgacgaaa aaataattga ttttaaagaa gcattgattt ttgcactttt aggatatttg
    14941 agaataaaca accataaaaa cacacttgcc actgtaacag gtgcaaaaaa agattctgtt
    15001 ggtggtgccg tttacttaat ggcttaaacc acaaactata attaagaatc ttatccgttg
    15061 ggattattcc tcatcatcaa acctgaaaaa atcttcatca ggaacctctt cctcttcctt
    15121 caagattttg ttcttccagt ttttttcttc acgatggtta gacatcttat gcttttttgc
    15181 atgatagttg tccgtttccc aattgtcttt tattgacttt ttttgttcaa ctctttgatt
    15241 tctcatgtca ttgaaaataa aaattagaat aataaactat tttactgcaa aactgttgtt
    15301 gaggtttcat gaaacagaag tttttttcaa gggttttaag cggaaaattt taatattttc
    15361 gtgcaaattt tttaaacata atgctggatt tactaaagaa atttgaagaa aagcgtcctg
    15421 aaatcgtttt tgaatggagt gatgctgaaa caggcgctga agggtggctg gttataaaca
    15481 gtctgcgtaa tggctctgcc ggaggcggca ctcgtatgcg taaaggatgt gataagagag
    15541 aagtcgagtc gttagccaaa acaatggaag taaaatttac agtttcaggt ccaccgattg
    15601 gcggtgctaa gtcgggtatt aacttcgacc ctgctgaccc cagaaaaaat caggttcttg
    15661 aacgctggtt taaagctatc aagccattgc tgaaatcata ttatggaacc ggtggtgatt
    15721 taaacattga cgaaattcat gacgtgattc caatctgcaa taaattagga atattacatc
    15781 cgcaggaagg cactgctgta ggtcacttta acgatggaga agaaaatatt aaaaaacgta
    15841 tcagcaatct gcaaaaaggt gtatctcttg aattgaaaga ttcgaacttg tcacctgatt
    15901 tagacaacaa atacagagta gccgatatga ttacaggcta tggtgtagct tatgctgtaa
    15961 aatattacta tgaattgtgg ggtggaaaaa tttctgagaa aagagctatc atacagggat
    16021 ggggtaatgt tgcagctgct gccggttttt atcttgcaca aatgggtgta aaaattgttg
    16081 gaatcattga cagagatggc ggtttaatca acgaaaaagg atattccttt gaagaaataa
    16141 aaactttatt caacaatcgt aagggaaaca aattgcatgc tgataatatg ctatcaccac
    16201 aacagatggc agaaaaagtg tggaaccttg atgcagaaat ttttattccc ggtgccgctt
    16261 cgcgattggt aacgcaagaa caaatgcaat cgctcatcag caaaaaactt gaagtcattt
    16321 catgtggtgc caacgtacca tttgccgata aagagatttt cttcggtccc attggaatgt
    16381 actgcgacaa gcatgtaagc gttattcccg actttattgc taattgtggt atggctagag
    16441 tttttgctta cttgcacaat gcaaatgccg ttattgatga cagattgatt tttgatgatg
    16501 cagcaaacac tatcaaaaat gcattgcagg aagtgattgc aacacataaa gataaaaaag
    16561 gtattgcttc tgctgctttt gaaatagctc ttaaaaaact gctgtaatgt ttatagatgt
    16621 tctgcaacaa aggatattag gaaacaccgt tgaggattat ctgtggtttg caggacttat
    16681 tctggcagga cttattttca agaatcttat ttccagaatt ctgacttttt tactcttcaa
    16741 tgttttaaga aaatatttta aaaccattgg tgtaaaaaaa tttgtagaac tgacacaaaa
    16801 acctttctct acaacactgt ttcttatttt catttacatt gcctgtcgtt atgtgcattt
    16861 tcctgatgaa tggaaacttg cttcagagag tgagtttggt gtgagaatga caattaaatt
    16921 gatttttcag attgctacag ttttttttat cacctggaca ttgcttcgga taattgatta
    16981 catcggtttg gtaatgcgtg cacgtgccaa caaaacagaa tcgaaaatgg acgatttgtt
    17041 tgttcctttt tttgtatcct ccattaaagc tttaacagtt atcatcagtc tgttttttat
    17101 tttgggcgtt gtgtttaaac tcaatgtagc cagcataatt gccggtttag gtattggtgg
    17161 tttggccatt gctttggctt ctaaagatac attagaaaat ctgttaggtt ctttcttaat
    17221 ttttatggac aagccttttg tggtaggcga tttagttaaa accgataatc ttcgtggcca
    17281 tattgaagag gttggatttc gatcaacaaa agtacgtacc attgaaaaaa cattggtgat
    17341 agttccgaat aaaaaaatga tggatgctga attagaaaat caaactgaaa gaacttttta
    17401 ccgtcatagg tttgttattg gtttggtata ttctacaaca gccgatcaga ttcaaaccat
    17461 tacgcaacaa attctggata ttcttaacga gcacccattg gttaatgaaa atccaaatgt
    17521 tttctttatc aattttgggg caagtgcttt agagattgag gttatttatt tcgtaaaaac
    17581 gcaagagttg agcgatttcg aaaacacaag acaagaagtc aattttgcca ttatgaaaat
    17641 tgtggcagcc aataaaagtg actttgcatt tcccagcact tctgtttata ttgaaaagga
    17701 agtgaaataa ccaaccattt tttggaattg agtaacagtt tttaaaattc tcccaagaaa
    17761 gaaaataccc acgctatcta tttccaatag aaaaatcagg ctgctttaaa agtttctttt
    17821 aaccataaaa gcacaatttg cgtctgattt tatgctactt ttaaaacagc ctttgattaa
    17881 acattactga ttttactgaa ctatcaattt tctgatggtt tttgtttctt cattgctaag
    17941 cgttattaaa tacacaccgg gaggtaatct atgatgcacc atccatgtta cttggttttt
    18001 gacagtttct ttatagactg taacacctgt tatattgact atgtttaact gatattgctt
    18061 ttgtgcttgc agccctgtta ttgtaaaata atcttttgaa ggattgggat tgacagtgaa
    18121 atttatggtg tgagctattg ctgcaataga aaccgttgta tccgttccta actttaccaa
    18181 ccaacaatca ctggaaggtt gtgtggtaat atgattaccg cttacatcgc catcgttgga
    18241 ataggttctc gctgctccaa tataaccctt gtcgttggtt actctaatga caccaaaata
    18301 ttccgaacct gtaccgccca aacacctttg ccatactata ttgctaactg tatctgtctt
    18361 tactgcccat aaatcttcgt aatgattgcc cactacatca ccatctatgg atttaaccaa
    18421 gccggctaaa atataacccc catcagcagt ttgctctgca ctgtaaaagc cttctgtatc
    18481 agtaccacca tagcaacgct gccattgcaa agtacctgta gcattgagct tgaatgccca
    18541 cccatcataa tacctataaa aattagaatg aaagcccact acatcgccat catcagaatt
    18601 ggttgcaccg gcaaccaaat agcccccatc agcggtttga atgatttggt tccctccatc
    18661 ttgccaactg cctccataac aaccttgcca ctgtatgttg ccgaaagcat ctgttttaac
    18721 tacccagaca tcatcccacc aattaccggc tatggtatca acatgccagc cacttacatc
    18781 gccatcaaca ctgtatgtac tgcctgtcaa aatgtagcct ccatctgttg tttgtatcac
    18841 cgatgttcct cgctcaccgc gggttcctcc caaacacttt tgccataaca gcgagcctgt
    18901 attactgatt ttaaccaacc aaacatctga tgacaggcca ccatgtagtc cgctgacatc
    18961 tccatcaaca gagcctgttg tgcctacaaa acagtagctg ccatcactgt tttgaactat
    19021 atcccatgag ttgtccacat ccgcaccacc atagcagtgc tgccattgta tcgtgcctga
    19081 tgcatctaat ttaactatcc aggcatcact ataacctaat gaatcatgat agcctgaaac
    19141 atcgccatca ttgggcgtat ctgtcgtacc ggctattata tagcctccat ctaaggtttg
    19201 ttttatcctt cgagctctat caaatcccga accgcccaga catttctgcc attgcaggat
    19261 tcctacagta tctgtcttta ccacccagat atcattacca tggttgcctg aaacatcacc
    19321 cccattgcca acggtggttt cgcccaatag cacataaccc ccatcggaag tctgacctac
    19381 atcccaggcc atttctacta atgcacttcc aaaacaatgc tgccaggcta tggtaggctg
    19441 cccttgtgct atattttgaa tgcatgtacc taaaaacaaa atgatactaa ctttaaaggg
    19501 caacatgcct ttccactcaa atagatttgt tttcataact tattgatttt ttgagtttgt
    19561 gtttttcctg tagtagtatt agtaatttgt agtaaataca gacctgaggc catctctttt
    19621 gacggactcc aaatatactc tttcttttgc acattttcaa ttttttcaat caccttaccc
    19681 tttccatcta taattgaaat ggaataaatg tcaagttctg atccttctaa gttaagaaca
    19741 accttatcgt taaagggatt gggaaacacc tctgatgggc tctgattttc agttgctgca
    19801 accctgaaca acgtgttgaa tgaggcactg taaatatttt gtttgaaata cacatcttgc
    19861 cctccgggag caccggtgtc atcattataa aaagcagcca gaaagttgct aaactgtagt
    19921 gcgcgcctac ctgatatgga aggtgctaat tgattctttt tggtggaacg gttaaccaca
    19981 aaataatact ctgactggta tgaaaaggtt tgcccatcca tattcatgcg aatacctatg
    20041 atgtcttgtt ggtcgggcac tgatacagcg tagcctatgg ggtcaacctg ccataaaata
    20101 aaaatataat cgcctacata gcttataaca ggatagctgt tttgataaat agtaatatca
    20161 ttattattaa atggtgatgc caaggagcca tcattgtaca catagggtgt gctgttgagt
    20221 ggatgatacc aacttgtagc aaatccatgt atttctgttc cacctgttgt agcattgatg
    20281 gtttgtgcca caacagccca gttatcatag ttgtaatagg tgtcaaaaaa agatgaaatg
    20341 cggggaaggt cgtatgcata atcagtgttt gaataattta acacataatt agtcggtgtg
    20401 gtaatctgtc ccaacttaat tacaagccaa ttctgtttct ccaccgtaag aatattattg
    20461 gtgccgtcaa attcattgta agtaaaataa acattatttc tcagtattgt tacatccgga
    20521 gctgagtaga tacttgttcc attgccctga agaatcacat ttttactaat atcaattaca
    20581 ggcttcgata aattaaaatc atagtatcct gcagtagcaa acactgccaa ctgatcatct
    20641 ttattccaca ccagcatcca acgatcgcca gtgctgctat cccaaaaacc atcaatttta
    20701 acgttgatgc ctccttggta gattttaatc gtagaatacg aattaaatga gttagttgaa
    20761 ctcctccatt caaataattc tgccataata taatcgttgt atgtatcttg atatacagca
    20821 attgcccatt cacctgttga catcagggtt cacgacaatg caattaggta tgccggcacc
    20881 aagtagggta caaccggtat ttccgttacc atcgtcccaa aaaagtccgg gatgagcacc
    20941 atcatataca atgactgagc gtttggtgct gcctaatggc gattcaatgt agtcgttgtt
    21001 ggtatttacc gttccgctta cataacaacg tgagctaaca tcagcattat tggcaggata
    21061 aaacgattgc ccataacttt ggtgccattg aactaataat acaaatgtga tggttgccat
    21121 cagacttgca atgagattac tttttttcat attaattgga tttttaaaat agcctactct
    21181 tttcaggatt ttcagcttcc tcctatttta tcaatatttt agaagtatcg aatctgaagt
    21241 ggataaggtt ttactgctct ttttaagaaa agagtttcag gtataaataa taaaaccaca
    21301 cctcctaaaa agtgtgaact tgacaaaaag ataaaatgct ttagttcagc ttgccaaaat
    21361 ttatcttggc tgcttggctg tattaattga atactttcat gcataacttc aattaatgct
    21421 actttatatt gtattgtttt cattaccaag ctgtgaatag tatggcaagt ataagaaaaa
    21481 aatttaaata ttacgttggc aataagaaag attactcaag aaaattttct ttttatcact
    21541 tattccctct ataaaataag agcggggctg gtcatgctga ccagccccgc tcttataaac
    21601 tttaatttat ctttttactt ctttaaattt tttccaataa atgcagctaa atcttttcct
    21661 gctttagcat aagactcaga tagacgtgtg ccggtatcat aatcatagcc accgaaggta
    21721 cgacctggaa atcttaaccc ttgcattttg caaattggct tatcgggatt ggctgtttca
    21781 tacaccgtta tttcaagatt gattgatgca ttctttctgg tgacataaac attaaaacca
    21841 ggctctgtaa aagttgtact cactttcatt gtatattttg catcaggaaa ttcaccaatt
    21901 aaaatcggag attttttatt gaataactct acaaaacttg gttcaaatct gtttttacga
    21961 tctgctaccc agctttttgc ccaactgtct ccacgacccg gttcatttgc attatactct
    22021 cttgtttttt tagctacata ttcttcttcc ttcgcaaaag agcctacacc catattacta
    22081 taatcataga caacattcag tttagtttct ccttttagtc cggccaaact accgtcatga
    22141 tattaatttt ctgagcagaa gctgtgccca caactgctgc aataaatagt aataacacaa
    22201 tttgtttttt cataacatat tatttttaac aaaggtaaaa cgttattgat gaaatttata
    22261 tgttaaatat tatataataa tgttaaagtt ttattactta gtcattgtca ttattcttca
    22321 atttgataac tctttccgct taagtcaaat aaaattcaga aaagtttttc taacgctttt
    22381 acaatgttag tataaatacg ctgttctata ttttcgtctg ccggttttac aaaagatttc
    22441 cctgtaatat tttcaaacaa ttcaatataa cgatccgaca ccatctgaac aatttcatcg
    22501 gacatttcag gaaccgtttg tcctgccaat ccctgaaaat tattttcaat caaccattgt
    22561 ctcaaaaatt cttttgacaa ttgcttttga ggttgttttg atttttgtct ttctgcataa
    22621 ccctctgcat agaaatacct tgacgaatca ggcgtgtgaa tttcatcaat aagataaacc
    22681 ttgccctgat gcacaccaaa ctcatattta gtatctacta aaatcaaccc acgctgtgcg
    22741 gccatctccg agcctcttct aaacagttgt tttgtatatg tttctgcctg ctcataaatt
    22801 gctgcatcta ctattccctg cttaattatt tcttccttgc ttatgtcctg gtcgtgacca
    22861 tgtgcagctt tagtagtagg cgtaattatt ggctccggca agacatcact ttcattcaac
    22921 ccgtcaggca atttaacacc acataacatt ctttttccct gtttgtactc gcgccaggca
    22981 tgaccggaaa gatatcccct gataaccatt tctactttca acggctgaca tggaatgcct
    23041 atagacacct gcacatccgg tgtatttaca atccaattag gtactaaatc ggcagttgcc
    23101 tgcataaaat gtgctgctat ctgatttaaa acggctcctt tataaggaat cggtcttggc
    23161 aaaaccacat caaatgccga aacacggtcg gtaacgacca tcacaagcca tttgttatca
    23221 atagaataga catccctgac cttgccttta taaaaagccg tctgtccctg aaatataaaa
    23281 tttgttgatg ataaagcttg catacaccac gatttgtggc cgtaaaatta ttcttatttt
    23341 tagaccatta aaattatttt atcaggagtg cgactttttg aagaaaaaac gcacttacct
    23401 aacattaaat ggatgagcgg tcaggagaaa atttcgggaa agcagaaagc cttcacagat
    23461 gcctatcagc aattacatga aaagctggtt cgctttgtgc agtctatggt ttataaccgg
    23521 gaggatgtta aagatattgt tgctgaaaca atgttggtag cttatgaaaa gtttgaaacc
    23581 attcgccata aagatgcatt attgagttat ttgtttaccg tttcaagcag gttggtgtat
    23641 aagcttcagg ataaaaaaaa gcaaatctat acagccattg acccggaaca agctgaacaa
    23701 gtgagtgatg aacataccgg tgccgatagt aaagttcagt taaaagagtt gtataatgca
    23761 ttagatcaat tgccgctaaa acagaaagaa gccatagtgt tgtttgaaat ctcagggcta
    23821 tcgttagcag aaatacagca gattcaggga gatagtcttt ctgctgtaaa aagcaggatt
    23881 gcccgggcac gtgagtcact taagaaaata ttagaagaag aaattgtata attgtaaatt
    23941 acttttttca ccatggaaaa taataagttg aataaattgt ttgatgcagc aagaaatgca
    24001 gatcctgtag tctcgctgga tgaaagctta gaacaattta acaaagaagt tgaagggaaa
    24061 gaaaccactt cggtttactt aaaatggcta aagcctgtca ttgccgttgc aacaattgcc
    24121 gttgtcgtag gaggaattta ttttttagtt cagaatcaag accagcaaaa cgcgcaacaa
    24181 tcaccggaga atacagcaca gacagcagaa ccaactttac aggcaacaga aacacaacct
    24241 gatgctgata ttgaacaacc tcagtcaaaa gacgaaaaca tagaagcagg aacatcttca
    24301 acaaaaacag aaatcacaaa tcctgctcct ataaaaaata aagttacaca tgctgttggc
    24361 gcaaatgatg catacattgt tgaaactcaa accgtgaata ttaataatga aaatggtgct
    24421 tttaaaattt caatcagtgg tgataatgtt gaacgaattg ttttaaatgg caaagaggtg
    24481 gagaaatcca actggtcaaa ctatcaggat gaaatagccg aagctatggc agcaaaaagc
    24541 actttgggaa taaacaaaga gaatgctgca aacaagaaat ttgttgatta tatgtttacg
    24601 accttaaaag aaaatggtct cataaaaaat aatcttgcag taatcaaact cagcaacgac
    24661 atgttgctga tagaaggaca ggaagcagac tctgctattc accagcaatt attaaaggag
    24721 tataaaaaaa ttactggtaa ggatattggc ttacggacac tttattttaa ctgataactt
    24781 ttaactgcga ctgaattttt tgggctttta caattgcctc atcaacggta gctgccgtta
    24841 cagttacatg tcccatttta cgaaaaggct tagtaatttt cttaccatat aaatggatgt
    24901 ggacatcacc agcgtttaac gcttcttgca cgccagaata aattgccttg ccattagaat
    24961 ctttatcacc taacaggtta atcattgcag caggcaaaac agatgctgta ttacctaacg
    25021 gaagattaag tatagctctt aaatgttgct cgaactgaga ggtatgattt gcttcaatgg
    25081 tatgatgtcc gctgttgtgt ggtcgtggtg caatttcatt aatcagcact tgtccttctt
    25141 ttgtaacaaa catttctaca gcaagaagcc caaccatgtt cagcttattg attaaatctt
    25201 ctgctattga aattgcctta tgttgtatgc ttgccgaagt ttttgatgga gaaaaaagta
    25261 attcaactaa attagcttct gcattaaact gcatttcaac aacaggatat gattttattt
    25321 ctcccgaatt atttcttgct gtaataactg caatttctgc tgcaatagct actttacttt
    25381 ctattacact tggtgcctga aacatcttac tcatgccatc agcatcttta acaaccagca
    25441 caccttttcc gtcataacct cctttgcgaa gtttttgaac aaatggaaac tccggcttta
    25501 tctgttcagg cttatctact aaataaaatg gggctgtggg taaattattc ttctgataaa
    25561 attctttctg aagtccttta tcctgaacca tttcaagaat atgtggttga ggatacaccc
    25621 ttttacctaa cttttccagt tcgtacaaag cttcaatatt gacatgttct atttcaatgg
    25681 taataatatc acaatcatta ccaaaggcca tgacagcctg ataatcgcga aaatcgccat
    25741 gttcaaatgt tttgcacata tagcgacaag gtgcagattc atcaggatca agtacatgaa
    25801 ttgaaagatt gtaatcggca gctttttgca acaacatttt gcctagctga ccaccaccta
    25861 aaactccgat aactatattg ggattgaatg tattcataat taacgctatt tattaaacaa
    25921 aaataaagaa cctgtggcaa tgaaaacaaa ataagctaag aatagaaact ccgtcagcca
    25981 tagggaattt atttttactt tagccgcacc caaaaaagtg gcaatgaacg aagttaccat
    26041 acttgataaa acattcacta tcatgattcc gcgaaagaaa atcgaaacca gaattctgga
    26101 gattgctcgc gacttgaatt ctgaatacaa agacaaaaat cctgtgttta ttggtgtgct
    26161 taacggtgct tttcttttta tggccgattt attccgcaac attgagactc cctgtgaaat
    26221 ttcttttatc agagtatcat cttatagcgg tactgaatct accgggcaaa tgaaaaatgt
    26281 agtcggtctt tcaggaaata taaacaatag acatgtcatc attgttgaag atattgttga
    26341 taccggtgag acagtttcat atctgatgca ggaaatatcc aagcatcaac cggcatcagt
    26401 caaattagct acattgcttt ttaaaccaga ggcattgaag aaaccggtta ctgttgatta
    26461 tgcagggttt gaaattccac ctgcttttgt tgtaggttat ggcttagact atgatggcta
    26521 cggacgaaat cttcaggaca tttatgtact taaatcataa tatcggataa aaacgaaatg
    26581 agaaatttat taccagaacc tttttaattt attattatgc tcaacattgt actatttggt
    26641 cctccgggtg caggcaaagg aacacagtcc gaaaaactta ttcagcatta tggcctcatc
    26701 catttatcaa ccggtgattt gttgcgaaac gaaattgcag ggcaaactaa attaggtctt
    26761 gaagctaaaa aactgatgga tcagggtgta ttagttcccg atgaagttgt tataggaatg
    26821 attgaatcaa aacttgatgc caacccaaca gccaaaggat ttatttttga tggcttccca
    26881 cgcactactg cacaagcagc agcactcgat aatttgttgc tacaaaaaaa tactacaatc
    26941 aaaatgatgc ttgctttaga agtaacagaa gttgaattat cgaagcgact attgctacgt
    27001 ggcaaagatt ccggtcgccc tgatgatcag aatcctgaag ttattaaaaa gcgcattaac
    27061 gaatacaaca ataagactgc tccggtagct gattattatc gtaaacagaa taaatattac
    27121 ggcatcaatg gcattggaag cattgatgaa atttttaatc tgttgtgcaa agcaatagat
    27181 aagtaacatt attattaaac aaaaaaacaa aatgaaattt tttattgaca cagccaatct
    27241 ggcacaaata aaagaagcaa atgacttagg tgtactcgat ggtgtaacaa ccaatccatc
    27301 attgatggct aaggaaggaa taaaaggcaa agctgcagtg atgaaacatt acgttgatat
    27361 ctgtaacatt gtaaaaggcg acatcagtgc tgaagttatt gccaccgact atgacggcat
    27421 tgttgcagaa ggcgaagaat tagctaagtt gcacgaacag atagttgtta aagtacctat
    27481 gataaaagaa ggaataaaag ccattcacta ttttgcttca aaaggaataa agaccaattg
    27541 cactttagtt ttttcagcag gacaggcatt gcttgctgcc aaagcaggag ccacctatgt
    27601 ttcgccattt attggccgct tagatgatgt gagtacagat ggtttgcaac tgataagtga
    27661 aatccgtcag atttatgaca actatggttt tcagacacaa attctcgcag ccagcattcg
    27721 ccatgcaatg catattataa actgtgctaa gttaggtgct gatgtagcca cttgcccatt
    27781 gaatgccatt accgatttac tgaaacatcc actgacagac atcggacttg aaaagtttct
    27841 tgctgacgca aagaagtttg cgaattaact tttattgtaa tgaaaaaaaa caaagaagtt
    27901 gttgctaaaa aaagtaacac aaaaactgcc aataaatctt ccagcgagac aactttagta
    27961 aaacttgaga actttttatt gaagaatgag acaataatat ttttttcaat tctgcttgta
    28021 tcattgatat tttccgtttt gctttttgac ataaagatga gtgaaggaaa tgatgactct
    28081 gattatattg aaggtgcatt taaacttacc aaagatttta gtagttatta cagtaccaaa
    28141 gcaccactct atcctatatt tttaagtttg ccggttagca tttggggcat taatcttcca
    28201 attctaaaat cactttctat tttatttagt ctattgaact taactgcaat gtattatgca
    28261 tttcgcaaat taatacctta ctcaatcttg tttgctgtcc taattttcac cggaataaac
    28321 agttactttt tatactttgc aagccaaacc tattctgagg cattcttttg ttttttacaa
    28381 gcattgttat ttattactat acggcctatt cttattgcct ctaaagaaga caacatacct
    28441 attgctaagt atttagtttt tggaattact cttaccttgt tatcattaac aaaaaatatc
    28501 actgtgggag caattgctgc agtagcattg tattttgtct ttaatcgtga ctttaaaaaa
    28561 ttagcttatg cttttggttc atttgttgta gtgcgtggta tttttgaagt tattaaatct
    28621 tttatctggg gttcacaaaa tcagttcagt aaccagtatc agattctgat tcagaaagac
    28681 ccatacaatg catcaaaagg tgtagatgat tttggaggat ttgttgacag acttttttca
    28741 aatacaagta tttatattgg taaaaggctt tatcagattt tgggatttgt ttcaccggaa
    28801 aatacaaaaa ccaattcagg gctagtgttg attacatttc tttttgttgg cattggtttg
    28861 tatttcattt ttaaaaataa agccagaatc atgcaattta cttttctgta tgtactattg
    28921 atggcaggat taaccttttt tgttttacaa acttcatggg atcaaccgcg catggtttta
    28981 gtttacgtac catttattat gatgattatt ttttatgggc tatattatgc ttttcaaaac
    29041 actcctgcac tgcgcagctt tgtttttctt attgttagca tcattataat aatgagtagc
    29101 ctgataaaaa cagctgataa gtctgttgcc aatttaccga tattaaaaaa gaatatgggt
    29161 ggcgatattt attatggtta tacagaagac tggacaaact ttttaaaatt gagtcgttat
    29221 tgtggtgaca gtcttcctga aacggcctat gtagtttcac gaaaagcacc tatgtcgttt
    29281 atctattcca atggaaaatc attttatcct atttatacag taccgagcac tgacccggat
    29341 tctgtacttg cattgttaaa agaaaataaa gtaactcatg tgttgattgc aagtttaagg
    29401 cgaaacccga agaagaatga tggctatgtt atcaacacat tgcatagaat gatggggcct
    29461 gtaggacaaa aatatcctga taaattaaaa ttagtgaagc aggttggaac aagtgagcct
    29521 tcttacttat atgaaataaa atattgatga gcgatagaat ttcaaacgaa ttagaacatg
    29581 gtaaaaaact tctggaaggt ggcgcagaag caatatggaa ctgggacagt cctgcgggga
    29641 aagtaagagc tgacagacgt gcagatttaa tagcccaata ctcacttgca gataaaagca
    29701 gcactgtatt agaaataggt tgtggaacag gacttttttc aggtaaattt tatcagcgca
    29761 caggtgcaaa aattattgct actgacattt caccggaatt actaaatgtt gcaaaagaaa
    29821 aatatccgga agtagagttt gtgttggcgg atgccatgca aatacaattt gatgcaaata
    29881 cttttgatgt tgttttcgga agttccgttt tacatcattt agagtttgaa cgctcgctga
    29941 atgaaatttt cagagtacta aaaccgggag gtcgtatggt ttttgcagag ccaaacatgt
    30001 taaatccaca aatatttatt caaaaaaata ttccatttat aaaaaaatgg cttggtgatt
    30061 caccggatga aacagccatt gtacgttggt ctttcaccaa attgttaaga caaaaagggt
    30121 ttaccaacgt caatattttt ccttatgatt tcttacatcc tgttgtgcct ggcttcctga
    30181 ttacaaccgt taactcaatt ggaaaaattg tagagaaaac acctttatta aaagaaattg
    30241 ccggttcagt tatcatagtt gctgaaaaac aacgttaatt gaccaaaaat cttttgctga
    30301 ctattttatt attttttaac aaactaacta cataaacacc tctttctaaa ggacctgttt
    30361 ctatttgatt ttctgtaaga tttaaagtag cactatacaa agtcttgccc aaaatatcac
    30421 aaatggttaa aacgcctttt ccatcatcat ttagtttaat tgttacgccc ttagtactta
    30481 aatttgggta aataaaaaat gttcccgttt gtttatagtc atcaaatcca actgatgatc
    30541 cacaaacgga aacggcatca ataaaattcc ccaaacttgc caaatttcct cctgaataaa
    30601 cagaattaaa actaatccta taataacctg atactggtat tgtgtagttg attacataat
    30661 gcccccatga ggaagtgcta tcggcaaaat atcctaaaac tgtaggaaga ttattttgag
    30721 ttcctaaaga aacgctcatg gtatcaatac caaatcttgc tctgtgagca aaactaattg
    30781 tcagagaatc tccggctatt aaagaaacat cctgataaac tgctgctact tgatttgcat
    30841 taatttccat aaactgatta ccgctatagg ctgttacacc attataaccc gactcccaca
    30901 actccatcaa actatctgtg gcagttgttt gccaacaacg cattgcatta ttattgacaa
    30961 aatgaggtgt tgaaatcact aatgggtttt caaaatcacc attacaaagt aattcgcaat
    31021 tctgtgaaaa tgatttgaag ttgctaaaaa gcattgcaca aatcaagttc aaaacaacct
    31081 ttttcatagt ttacaaattc tattttcaaa agcacaattt attacccaga aacaaatatt
    31141 atgccctact aaaaatctaa catatttaaa atgattcaat aaattgattc ttttgaataa
    31201 aaacaatttg ttgcataatc attttatttg ccctatgttt gagttcgtca aatcaagctt
    31261 atgaagccaa caatactggc catttttctt ttctgtttca gcaatatatc tgcccagact
    31321 tatcttactt ccggaggagc acgtgcatcg gcattgagcg atgctgctgt caccattaac
    31381 gatgtatttg cagcatttaa caatcaggcc tgcctttcaa tgatagaaaa accggaagca
    31441 ggtcttgcct tgcagaaccg ttttggcatg aaagaactca acacattatc tgccgctttt
    31501 gctttgccgc tagcatccaa caaaggtgtg tttgcgctca gcgtcaaccg ttatggctac
    31561 aagcttttta atcagaataa aataggtttg gcttttgcaa aaaaactaag tcccgttttc
    31621 agtgcaggtg tgcaggttga ttatctgaac acacacattg acgaaggcta tggaaacaaa
    31681 tcatctttca ccattgaagg tggtgtgctg gcagagttta ataaacttaa agcaggagta
    31741 catattttta atccactaca tgtaaaaatt accgattatg atgatgagcg aattcctgta
    31801 atcatcaaag caggtatcag ctactccttt tctgaaaaag ttctggcagc agcagaaatt
    31861 tttaaatcaa ttgacagcaa agaaaatttt aaaattgcgc tggaatatca tccagttaag
    31921 gcattatatg ttcgtggtgg tgtgagtaca gaacctgttc agcttacatt tggtttcgga
    31981 ttaaagtttt cacaatttta tgctgacttt acttcaggtt attttgaacc attaggctat
    32041 agtccgggtt tggctttgcg ttatgcgttt taatcatgaa aacaattcaa ctgtttttta
    32101 tttcattttt agtctgcaac ctaacatttg cacaggtggt gccaccacaa gatgacagta
    32161 aaattgaaga gaaaattgaa aatattgcca atacagcaga tgagtctgct gacctgactg
    32221 aactgacaga gcaacttcag caattagctg ccaacccaat aaatttaaat aaagcaacac
    32281 gtgaagattt gttgcagaca ggattgttta gcgaagtgca agctgatgct attatcgaac
    32341 accgtgaaat ttcaggaaaa tttatttctc tttatgagct tcagtccata gactatcttt
    32401 cattagagga cattgaagaa attttacctt atgtaactgt tggaaatacg gaagatatta
    32461 aagcaacatt aaaaaaaata ttttctgatg gtaacagcag actcattgtt cgtggtcaac
    32521 gttatcttga aaagcaaaaa ggctttcgcg acagcacagc aacagacaaa gttcatcctg
    32581 cgtatcccgg cagcccatgg aaactttata cacaatatca atatcgctac aaacaaagat
    32641 tcagctttaa tttaacagca gaaaaagatc agggcgaaga gttttttaaa ggttcgcaaa
    32701 aaaatggctt cgactattac tctgcgcatt tagctattcg tgattttgga attattaaac
    32761 gtgccgttat tggtgactac gatttatttt atgggcaagg attaacacta tgtacgggtt
    32821 tgtcattcgg caagtcgcct gacgttgctg cagtacgcaa attttc
//
LOCUS       DAOQNP010000026        31295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_456_length_31295_cov_8.173816, whole genome shotgun sequence.
ACCESSION   DAOQNP010000026 DAOQNP010000000
VERSION     DAOQNP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 31295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31295
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_456_length_31295_cov_8.173816"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6127)
                     /locus_tag="PKY09_05485"
     CDS             complement(<1..6127)
                     /locus_tag="PKY09_05485"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12342.1"
                     /translation="MVLSLTGNSVGSGQTYQWQESTTIGGPYTNIGASSNSSVLNILA
                     STTKYYQCEVTCSGNSQLSTPVLVTVNPSFPGGNYTINSALPTSGSNFQTFNDFKNAL
                     NCGIAGAIVVNVVAGSGPYNEQVEFGEITGVSSVNTITINGNGNVLTYGATVSTAPGT
                     LVLNGTDYMTVNNLTVEGTGTTYALVCHLWNNADNNTFSNCTFNAPVNGTSTSMVPFS
                     ISGSATSGTASGNSGINNVITGCTMFSGYYNTAIVGNSSLPSTGNQVINCNILDQYNY
                     ATYFLYQNGLVFRGNTLSRPTRTNLSTYYGIYLSTGNTNALVERNKIRNTFATNPTST
                     SIQYSIYCTIDATLGNENKFSNNVISDINSNGTVYGFYMSGSDYWKAYHNTISLDDAS
                     STATGTTYGFYCTGSLAYDVRNNIVSISRGGTGTKYDVYYSNPSAATSDYNDLYMNAS
                     AGTNNIGYNGTAYTTLAAWQSGSGKDANSVSVDPLYVAPGTYDYTPSNGLVNDAGTPV
                     GVTDDINGAPRSLTSPDMGAYEFSLGGLDAGITWFTPTSPSSPGLKTITVNIDNTQAQ
                     TINSIQLSYSDGGAPVVQTFTGLGLTSGNNTNLSFTTQYNLVSSVTMTVNILAVNGGA
                     DATSGNNTDSYYLCLAPSGTYTINSALPTSGSNFQTFDDAIQALSCGITGPVVFNVVA
                     GSGPYNEQVVIPEISGASSVNTITFNGNGNTLSFNGVVSNPNTLALNGADYITFDSLN
                     IEATNGTYGIACHLYGGADSNTFSKCTFTTSITGTTTVLAALVVNGSATTSTTTGLSG
                     SGNVFSDCEMIGGYYGTVITGLTTSPYNLDNQIINSTIRDFYFYGIYHSYCRNTIVSN
                     NIIERPNRASTSTTYGIYMSTSSYNILVEKNRIRKLFDAIPSSTSTAYCMYNTASGAA
                     GFENKWINNLVYDIESSGALYGLYCPSYNYVHIYHNTISFDDAGATGTTSTYGIYAYG
                     TSTEVKNNIISITRGGTGTKYCLYFGTNTAVASNNNDLYINAPSGSNNIGYSSGAHAT
                     FANWQAANGGAWDQNSQSVNPNFANIALENYKPTNVALDNIGAALGVTDDITGALRNM
                     TTPDPGAYEFAAANKDIGVSAFIGPNPDGCFTNAETIVVEIKNFGVQNLDFSIDPVTV
                     NCVITGPVNTTVSATLSSGTLASGATMNVSLSPTVDGTANGTYTLNASTSMVSDGDNT
                     NDALPAISITVGPIAGTASISVSSLCVSGDPVLSLTGHYGGDIQWQQSTVSATGPWTN
                     VGTNDSTYTPGTVTQSTWYQALTVCNSNTATSNVVAVTVNNPQINSLVPGTRCGYGPV
                     TLGINANMGTTGNWYTNSSGGTPIATGTTYTTPNLNTTTTYYVSPSSGGANGLTIPGD
                     GDWDHVTSSGSYQTTLITGSFMILTVTQPFTLESFDIYPSAALGTAFGIEARTTSASG
                     PMFESYSGNTTVVNSGTPTVAQTVNVNWVLPIGTYYIGFVGTNPNSWRSGSATHSLPW
                     VLPGYASLDYDLTPNYQYYFYNLKISTGCEGVRVPITATVTAPPALSVTTPDPTLCPG
                     GTTTVNVSSINDPSYTYSWTSNPSGFTASGTGPHNVSPTITTQYIVTATDNTVGPNAG
                     CVAVDSVEIVTGATLTAGTVTSSDTAFCVDGIPTLSVTGADGGTIQWQQSTVSASGPW
                     TNVGTDDVVYAPGSVTQTTWYQVMVHCQSSQVYSNVVQVTVNNPQISNSNGDTRCGPG
                     TVTLSATPSSGTVNWFTQPTGGTAIATGNTYSPNISSTTTYYASASTGGGTESAARPT
                     YLTTANTRGNGWGLVFDVVNSPIVLQSVTVYSVTTGGTMSVDLTDNNGVVLQTAGPFT
                     YPAGSVSSPVPVVLPVNFTVPVGTGYRILSASTSGGNLIRETTGISGTWPYTSASSNV
                     VVTSGYISGVTTSTYYWFYDWQVSAGCEGTRVPVVATVNPSPVVNISVQYPTICTGQS
                     ATLTATSSNPDYTYNWTPGGSGASIVVAPGTTTTYTLNAIDNTAGPYSQCTAIATQLV
                     SVVSANPPIPIANSGGDVCLGNDIFL"
     gene            complement(6290..6838)
                     /locus_tag="PKY09_05490"
     CDS             complement(6290..6838)
                     /locus_tag="PKY09_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12343.1"
                     /translation="MSLLIALLFAINVQAQTTVQIGTGNTLPSNTLYSPVYRFSATST
                     TTHCRGNILYTAAEMSAAGIPAGATITKIAFKKGNTANFVTPASNYNMLMANTSNTSL
                     ASGLAWTSILNTHNQVFQSSSFNIPNTIGWVDWVLTTPFNYTGGALEIAYEMQMTGNG
                     GATDKFLWEYSGTSGSSASLIV"
     gene            7300..7947
                     /locus_tag="PKY09_05495"
     CDS             7300..7947
                     /locus_tag="PKY09_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB12344.1"
                     /translation="MNAFADLSILIADDHVIIRRGLKFLLETHFGKISIVETDSSKGI
                     LSSLAKHQFTHLILDMQLADGNLLEIFQEIKQQNPHLPILIYSMSPEDIFAKRLNNLG
                     ASGYLNKQSSEEEVVKAMELFFTGRKYVSQKYFDMQHSATKGKSDNPFSNLSERETEV
                     LLLLLKGKSVKEIASYFDLKSNTVATFKARIFDKIGVNNLMDLQNSARAYNIPTV"
     gene            7953..10997
                     /locus_tag="PKY09_05500"
     CDS             7953..10997
                     /locus_tag="PKY09_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB12345.1"
                     /translation="MQKLRVLLIILLFSFSKLPAQNSYSIQNYTIKKGLPDNNILGLF
                     SEKNGYVWVVYGSGLARFDGINFKKYFTSETPYLNLFISKSANGDKFMIEPSGNIFQV
                     KDLSIDTIRKGKINSLNYINIKGTLPDLKTYLELSTPHVNITVDKNWILTPLIMFPLK
                     DSSYIVRTKKGIAHIKGKKNIKELNLEHLHNRKYISANGNIYVFVNDSEIYRVDVASW
                     SLVRCKLNGDLNSYKNFSSKITNTFWNFNNSDPCVQVDFNLYSFLPDSLNVNEINTIQ
                     LTNQLPNNCLITSIIYNADQNLILTGTDTRGLFVYKKRNFKSLAFSNPEPGTNNTYFC
                     QIALNDSTVYTDWNREFTINGGKPSKLHLTRNYSENIYIDKKGDYWYAENNTLVRYNP
                     KTKAKKYIYYTGDELPLSFYEEGDSLWVGLIKSFGCVIDDTLKHLKILDNEESNSNIF
                     QMLRYEGKLWICNYTGVYAYNTQNGKLDTLHDLALKYTYHFKVINDYLLIGTYGRGYY
                     MYKNGKTVKVPNGRFEALKQVHNFVLDSLGYLWMATNNGVFKTKFEELVHYFNDATYQ
                     ISYFHYSEEDGIINPEFNGGCDPNYLRLKNGYVSLPNVEGLVWFYPEKIEDPRSDYPI
                     SIDQFIADDSIYNSLNSILVPESVQSIRVEFSNPYWGQPENIFSEYKLEGYNKLWVNL
                     LPDQSAIEFSNLKTGKYKLHIRKQTGLSKDYTEATIAFQIEKKYYETIWFWLAMTGLA
                     LLIIIIVARLYSYNIRRKNILLEKNVVLRTSELQIAYNELSESIKIKDKLISIISHDL
                     ITPLRFITMVAKKGAGEDVEMDTKKHKELLGEIEFTTDKLRANAENILNWIKQQNKRI
                     ERQITSVAIGALAEDITDQFMHITYNKGIRLVSTISHDDIIQSDSNLLSIILHNIISN
                     ATKFTENGFIKISSRQNSHYEITIEDNGKGMSEKQLTRLSKLINKQPLNEEDEQSDNK
                     EGFGLGYVIISELIQILNGKLEVQSKLGAGTKVTISLPLIYH"
     gene            complement(10991..14146)
                     /locus_tag="PKY09_05505"
     CDS             complement(10991..14146)
                     /locus_tag="PKY09_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB12346.1"
                     /translation="MTTKQFLLMFLFYMPFFAKADTTLVSFSDSWKYFDSGYLPASNW
                     SQIGYNDSAWAIGNGEFGYGDGDETTIINYGGDPNNKFITTYFRKTIALNNPYDFASY
                     HLDLKRDDGAIVYINGTEVFRSNMAAGNVGYSTSAYTDCVDDGNALQSIVLVPADFLS
                     GINVIAVEVHQINNISSDMSFDLQLNAYNNFVPQLLRQPYLQMVADSAITIRWRTNVL
                     CQTAVLYGNDPDSLNFTMTDSVNVIDHEIRLTGLSPDTRYYYAVSDGINILQGDSNNY
                     FKTAPVKGMSKHVRIWSMGDTGINTIDQNNVRDAFLNYNDTLPIDLLYMLGDNAYYSG
                     TDQEYQDAFFQNHYEDILKNIPVFSVAGNHENYTANAITQTGPYYDIFTFPKNGECGG
                     LPSGSEAYYSFDYGNIHFIALETNTLSLLQTGSVMLNWLQSDITATQQKWKIVVFHNS
                     PYTKGGHNSDTETDLILIRQNIMPILDQHKVDLVLCGHNHVYERSYFMDGHYGLSSTF
                     DSTMVVDSSYGQLPQPYLKKSSKDYKGLVVVQAGCSGVLDPVGPEWPHPAMKRYYDQE
                     LGSLLIEIEADTLTVTFVDNNVGAPQVLDAFSVVKECDVVPQLTVPVTKVCVTDDPVQ
                     LSATPIGGFFSGLGVVDTVFNPTVAGKGIHTIAYTYNDMYGCHVIDTKTIEVVGGVPS
                     QPSGIVGSVLVCPPSSNEVLSIQSLPDADSYIWTASAGVDFTSSNTDTLVNFDINAVL
                     PQYNISVSALNICGSSNPSVVSLTSVKSFPVSLTGNSVVCPGDTFVCSVNAFSSLSSY
                     WWQATDNIFLNGQSGIVNTFNTLQVNASVDSNFVYGRICVASKEACLKNNAVSCIELS
                     TKPPVPGVISGPAAVLGGSSGVSYSIVPVSGATDYFWTVTGDATVASGQFGNAITVDF
                     LPAFTGGYVGVKSRSNCDESVEVAKLIKSYVIQVAQNSVAQNKVANEYSHDRLISVYP
                     NPIGNEMHLRMLLKESSLTVLIILKDVAGKEVGKWVKKVFPDEDDITLDVSFLSNGIY
                     YLCVTSDNLHWNGVVLKQKNQ"
     gene            complement(14253..>16921)
                     /locus_tag="PKY09_05510"
     CDS             complement(14253..>16921)
                     /locus_tag="PKY09_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12347.1"
                     /translation="SCGTVVSSATIVGAAISVSISPSGSVNLCSGPVTLAANTGVGYT
                     YQWKNNGVDINGATNSSLEILSAGSYSVLATDSTGCASLSNVVTATGSSHVISALGAT
                     DICAGSKVMLSAQTGTGFTFQWYRNNGAISGATGSSYNASSHGNYYCRIVAPGCVGNS
                     NTIVVTVINNPTPTISANGSLNVCSGSSVILSTNTFSGVSYQWERNGSAVNGATSQLH
                     TTTLAGTYRVTQTANGCSKKSPAIAIKVVTGPTATITANGSVNLCNGQTVTLNANIVS
                     GATYQWLADGVDIAGATNQSFIASTSGNYQCRITTTCAALSNIVAVTASSMQISISPS
                     TTQTVCQGNNVLFSTSNEPGNNYQWNVDGNAIPGAISDSYSANVSGVYSVTITNGCGT
                     QTSQAVTVNVVPVTAAISPDAAQIICSGGSVNFTANTGFGNNYQWNLNGNPISGAISQ
                     TFNANVAGNYSVTISNSCGVYTSASVQVSVGSIIATISPDTTQTICSGDSVLLTANPA
                     SGISYQWYLDGNTIVGANASTYYAKSQGNYTVSIVSSCGNSTSPSVALTVISLSSSIT
                     PAATQTICPGSFILLSANTGNGLSYQWKKDGIMISGATQSTFLAEETGIYTVDVSNSC
                     GSLESNQVTVNAMQINASVNPAGTTTVCAGTPQTFSVLPTTGATYQWYRNNVAISNAT
                     NNSFNASSSGQYYVKVHLSGCNVVTSNTAILSVQTNTTPTVTVTPGTTLCNGQQATLS
                     SNIYSGVAIQWQHEKVNIPGATSQTYQTSIAGQYRVVHTYNGCVRNSAEKSIKFVSCR
                     SEGYETDLEMQVSAEVFPNPFKEVTNIKLNGFEISSTTITVTNILGEMVHRFHPDAES
                     IGWKRDGNAPGIYLMQITDGYNHRITQKIVIE"
     assembly_gap    16922..17021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17022..19525)
                     /locus_tag="PKY09_05515"
     CDS             complement(<17022..19525)
                     /locus_tag="PKY09_05515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNB12348.1"
                     /translation="MKLRLTILFMAMVCLANADQTIFPLNSVWKYLDNGTNQGTAWRD
                     SNFSDASWSSGNAKFGYGEGDESTIVSFGPNAANKFVTTYFRKSFNITNVAAFTAFEL
                     GVRRDDGVVVYVNGVEAWRDNINGTISYLTLAASDAADDGNSVLTANIPASLFVTGNN
                     VIAVEIHQFVVNSSDLSFDLRLTGLETPPPTNVTIFPLNSVWKYLDNGTNQGTAWRDV
                     AFSDATWASGPGEFGYGDGDESTVVSYGSNASNKFITTYFRKTISIADISAFTSMQLG
                     IKRDDGVVVYVNGVEVWRDNINGVVTYTTLAANDAADDGSSLLTANIPTSYFVNGTNV
                     IAVEIHQFVANSSDLSFDMSLTGMGNTPPAIPSVVIGSYLNVATPTSIIVRYRTDISA
                     MSKIVYGTTSGIFTDSAVSASPVTEHIVQLSGLQPNTKYYYAVKSGNNFLQNDPAENY
                     FITPPVAGTVKPTRIWVLGDMGFGSVEQNQVRDAYYNYTGNTYTDLWLWLGDNAYNSG
                     TDAEYQNNVFNNRYEKMLRQTVVWPAAGNHEMYASNAVTETGPYYELFNFPTNGEAGG
                     VPSGKKAYYSYNYANIHFIVLESTTASFRTIGGAMMNWLQADLAANTQKWTVVYFHHP
                     PYSMGSHNSDTETELIEMRQNFVPILEQYKVDLVLTGHSHNYERSFFIKNHFGSESTF
                     NNSNLVDGGSGAGATPYIKSASSNFQGTVYAVAGTGGILEGTSPGWPHNAMYSYSSST
                     YGSMVLDINGDTLYAKFIDNTLPNPTIYDQFTIIKQCDQQVTLAPYNNVCSGQSAFAL
                     SGGSPSGGTYSGIGVNNGVFDPSVAGIGTHTITYTYT"
     gene            complement(19611..20897)
                     /locus_tag="PKY09_05520"
     CDS             complement(19611..20897)
                     /locus_tag="PKY09_05520"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNB12349.1"
                     /translation="MNYTETLNYLFSKLPMFHRIGAAAYKNNLDNSIALDNILNHPHK
                     SFKSIHIAGTNGKGSTSNMLAAVLQQQGFKTGLFTSPHLKDFRERIRVNGKMIPKKYV
                     SGFVQQYMNEFDVVKPSFFEWTVALAFDYFRHCKVDVAVIETGLGGRLDSTNIIQPQL
                     SVITNIGWDHVNLLGDTLEKIAVEKAGIIKKNIPVLIGETTLHTKKVFLKKAIENAAP
                     IYFASSQCKVRLKKKHLEYLLLDAQLKHGEIYNNLKLSLAGDYQLKNVATVLSVIELL
                     KDSGFAIEKKNVKKALAKVQHLTGFRGRWQVLGTKPLLIADTGHNVDGIKTVIRQICK
                     ISFKKLHLVIGMVNDKDVMPVLKLLPKHAICYFCNAKIPRAMPAKELRSKAIEVGLQG
                     EYYTSVKHALLSAKKAATAQDLIFVGGSTFTVAEVL"
     gene            complement(20899..21762)
                     /locus_tag="PKY09_05525"
     CDS             complement(20899..21762)
                     /locus_tag="PKY09_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12350.1"
                     /translation="MEAPAIKRNSLLLTLVLHGILLFLLWWFTMHTKIPPFGGGGGAE
                     SANLGFVELSTGDIQPESENVSENPHPATATPPAVVQEQNYTTQETEESAVVEAKKEK
                     TPEKKVIKTVETPVKTEVKKVEPVVPKVNAEALYPGKKNKSASQGTSNTGSGDQGTRD
                     GDPNALYQGKGTGQGGSGSGGGEGDGSGPGKGSGSGPGFSYDLAGRSVRAKPTIYDNS
                     QETGKVVVSIKVDKYGNVIDAVPGARGSTTTSSNLFRKAKDAAFKAKFNQSPQGVEEQ
                     RGTITFVFLVQ"
     gene            complement(21764..22168)
                     /locus_tag="PKY09_05530"
     CDS             complement(21764..22168)
                     /locus_tag="PKY09_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNB12351.1"
                     /translation="MALNLRRRNKMTGEVFVGSLNDIMFFLLLFFLITSTMATPNVLK
                     LLLPNAKTSKPAVKHPVTISVTADMQYAVNNQVVVFDQLENAILAQLQGQEDPTAILK
                     VDKSVQVQGLVDLLDIGARNKIKMVLATNTVK"
     gene            complement(22199..22897)
                     /locus_tag="PKY09_05535"
     CDS             complement(22199..22897)
                     /locus_tag="PKY09_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNB12352.1"
                     /translation="MSILLQITQAVTDSATALQPAVKAEQSISFLDLVIKGGPIMIPL
                     GLLSVAAVYIFFERYFTIRKSSNIDKNFINQIKDFVHTGNVDAAKALCRNTNHPVARM
                     IEKGLLRLGKPIRDIESSIENVGKLEIYKLEKNLSFLGTIAGIAPMFGFLGTIFGVIK
                     IFYQISLENSLQIDTISGGLYVKMITSAAGLLIGVAAYAGYHYLISLVDRAVNKMEIN
                     AVEFIDLLEEPVKK"
     gene            complement(23067..25286)
                     /locus_tag="PKY09_05540"
     CDS             complement(23067..25286)
                     /locus_tag="PKY09_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNB12353.1"
                     /translation="MKRLISIVFFLLLLQNGFAQLVSIATARTSPAGAQVTVTGVCVN
                     GQELGNIRYINDGTGSIAVFSTQLNATVRGDAILVTGVLSDYFGLLEITTLTAFQNLG
                     GGNNLPAPIVTTPLLLDETTESHLVRIDGVVFNNGGGTFMGNANYAFTSNGENGQVRI
                     AANTNLVGQPIPSGAVSLIAVCSQYQTVYQLLPRDQNDIFISSSFYMTSPPVQQNISA
                     SGFDVNWTTNLPASSFIEYGLTPALELGILNGTSGSTNHTVTLAGASPSQLYYVKAFS
                     VNGNDTAKATVKIYMTASLSSGDMKVYFNKSVNNNYAWTPANNAIQLPGTFQDTIAAY
                     INRSQQSVDIAIYNFENSGTSQIVQAINDADNRGVAVRIIYDGGNVNSGLAQLNPGIN
                     MLPSPTTPPGYYSIMHNKFVVIDAFASNANYPIVISGSTNFTNAQLNNDANNLLIIQD
                     KSLAIGYTMEFEEMWGSSTLQPNPSNSKFGPDKKDNTPHEYNIGGNRVESYFSPSDNV
                     NNQIMTTIETANQQMQFALLVFTRFDLAYAAEDRIVNHGVDAYGMVDDTGSGGGQAYS
                     ILNAVMGSKLMLYNHATQTGLLHHKYVIVDQNDPSSDPLVLTGSHNWSTTANQKNDEN
                     TLIIHSRNIANQYYQEFVKRFTDNGGVLGLNETEQGMTVQLYPNPASEHVFICLNHTL
                     ENNYQFELFDVAGKQVMQLNLQQGNSGMISLGKLNKGIYFYQIRGVKLINSGKLIIN"
     gene            complement(25354..26775)
                     /locus_tag="PKY09_05545"
     CDS             complement(25354..26775)
                     /locus_tag="PKY09_05545"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNB12354.1"
                     /translation="MIKNIKITTAFAILAITLSTVSCVKKDDFDNAPSSNVDPDIVTN
                     ATVSSVVTLANATGPVQITSDLTFSAIIVADDKSGNYYKEMIVQDTTAGISILLDQPS
                     YFTSLFIGRRVFVKAKGLYIQAVNGTPKIGVLNAGAVAEIPAAEIGNYIVGGKWGLTV
                     VPAKRKLNALTDADLNTLVQFDSVAFASTEVGLPFGDAANKSTVNRNIQDCFGHSLIV
                     RTSGYANFATTLIPCKGGSITAIYQKYNATPQVFLRDMNDVPANIERCDGVVCTPVYV
                     SIDSIRTLFSSGTTTVPGGFHIKATVISDYTTSALDTRNVIVQDATGGICLRYSTAPN
                     FAMNSQYDFNVGGLTLAEFNGWLQINNMAGGLSITGLAPITPTVIDLATATANFEQYE
                     SMLIKVNNVTINNGTATTYGANGGSLTINDGSTPNTVVLYCRSAASFASSATPTTPVS
                     ITGILTQFSTTKQFQIRNLSDIQ"
     gene            complement(26802..29558)
                     /locus_tag="PKY09_05550"
     CDS             complement(26802..29558)
                     /locus_tag="PKY09_05550"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12355.1"
                     /translation="MTLLILPFLSSAQSGVIKGVILNENSVPMAGEVVVLNGTNYQSI
                     TDKDGAFTMSGVTYGTYSLSVQDVAYNGFKQEVVVNAESVDVGSVKLVYSPGTTTTEA
                     DIPVVTLDDDELQSNSAQSSVSSVLGASRDAFTSAANFNFSVARFKTRGYDTENFPTL
                     MNGIAEEDLTNGRTAFSSWSGLNDVLRSRESTSGLNPSTYAFGGVGGSYSIDSRAGRQ
                     RKQIQATYSLSNRTYDNRFMLTYGSGFNKKGWAYSASISKRWAKEGFIKGTYYDGFAY
                     YLGVSKVINKHEFDFTVLGSQYESARSSSNTKEVYDIASDHYYNSNWGYQNGEIRNAN
                     IGRSHQPVFTLNHEYKISNNTTWFSALSYTTGKRESTALNWFAADPRPTYYRNLPSWQ
                     TDSAAAAQVYNTLKANPDLLQINWDNMLLANDQNFQTVNNADGVAGSTVSGKQALYLI
                     ENRVMQTNRLSFNTTFNTILNDNITTSTGLSFQKQTSRFYKEAEDLLGAEFFVDIDKF
                     ADQDFQDEQLSQSDLNHPNRIVREGDRFGYDYEAHITNAKAWTQAVAKYNNIDIFGAI
                     QAAYTSVYREGNYRNGLFPDDSYGKSKTYVFPDVGVKAGATYKVNGRNYVFANAGYMT
                     RAPFFNNMYLSPRSRNTAVADLTSEKITSLEGGYLHIAPKIKGKLKLYYTNFRDGIES
                     RSYYLQGFGTNSYVNYTLSGIEKVHAGTEISVDANLGMGLSAMAVASIGQYYYSSRPT
                     ATITVDNTNTVQSTGEVVYMKNLHVDNTPQNAFTVGLNYRSKKFWYVGVNANCFSNSY
                     VEASPARRTGDIIDGVDENSQAWEKILGQERYDAQMTLDLSAGWSIKLNNHIKSLKRN
                     TFFLINLNINNLLDNKDIITNGYEQGRLDKSAVLSGRPDVFASKYSYAFGRTYMISFI
                     LRMN"
     gene            29854..31077
                     /locus_tag="PKY09_05555"
     CDS             29854..31077
                     /locus_tag="PKY09_05555"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNB12356.1"
                     /translation="MEKIKVLNPVVELDGDEMTRVIWKFIKDKLILPYLDIDIKYYDL
                     GMEHRDATDDKVTVEAAEAIKKYNVGIKCATITPDEARVKEFNLKQMWKSPNGTIRNI
                     LDGTVFREPIVCKNIPRLVPNWTAPICIGRHAFGDQYRATDFLTKGKGKLTIKFEGED
                     GTIEHEVFNFKGNGVALAMYNTDESIRGFAQSCFNVALQKGWPLYLSTKNTILKKYDG
                     RFKDIFQEVYEKEFKSKFEAKNITYEHRLIDDMVASALKWNGNFVWACKNYDGDVQSD
                     TVAQGFGSLGLMTSTLITPDGKTMEAEAAHGTVTRHFREHQKGKPTSTNPIASIFAWT
                     RGLDFRGKLDNNAALINFAKALEEVCVETVESGKMTKDLAVCIYGNETKPEHYLHTED
                     FLSVIDTNLKKKLQK"
BASE COUNT         9883 a         6064 c         5647 g         9601 t
ORIGIN      
        1 aaaggaagat atcattgcca aggcaaacat caccacctga atttgcaatt ggaataggtg
       61 gattggcgct tacgactgaa acaagctgag tggcaattgc agtacactgg ctataaggtc
      121 ctgcggtatt atcaatggca ttcaatgtgt aagtcgtggt tgtaccgggg gctactacta
      181 tacttgctcc actaccaccc ggtgtccaat tataagtgta atcaggatta ctacttgtag
      241 cagttaatgt tgcactttga cctgtacaaa ttgttgggta ctgaacagat atatttacaa
      301 caggtgacgg attcacagtt gctacaaccg gcacacgtgt tccttcgcat ccggcagata
      361 cctgccagtc gtagaaccaa taatatgttg aagtggtaac tccgctaata taaccgcttg
      421 ttacgacaac attactactg gctgaagtat agggccatgt acctgatata cctgttgttt
      481 ctctaatcag gttaccaccc gatgtgcttg ctgaaagaat acggtagccg gttccaacag
      541 gtacagtaaa gtttactggt aaaactaccg gaactggtga agaaacactt cctgcaggat
      601 atgtaaaagg ccctgcagtt tgcaatacaa caccattatt atcggttaaa tctacggaca
      661 ttgtaccacc tgttgtcact gaataaacgg ttacagattg aagcacaatt gggctattaa
      721 ctacatcaaa taccaatccc caaccattac cccgagtatt agccgttgtt aaataggttg
      781 gtcgggcagc cgattcagta ccacctccgg tagatgctga agcataataa gtagttgtac
      841 tgctgatatt aggagaataa gtattaccgg ttgctattgc tgttccacct gtaggttgtg
      901 tgaaccaatt tacagtacca cttgatggtg tagctgaaag agtcaccgta cctggtccac
      961 aacgagtatc accattgctg tttgaaattt gaggattgtt tactgtaacc tgaactacgt
     1021 tggaataaac ttgtgatgac tggcaatgta ccattacctg ataccaggtg gtttgcgtaa
     1081 ctgaacctgg agcataaacc acatcatcgg tacctacatt ggtccatgga ccggatgcac
     1141 ttactgttga ttgctgccac tgaatggtgc caccatcggc accggtaaca gataatgttg
     1201 gaataccatc tacacaaaat gcagtatctg atgaagtgac tgtgcctgct gttaaagtag
     1261 caccggtaac tatctcaaca ctgtcaactg caacacaacc ggcattaggc cctactgtat
     1321 tatcagttgc tgtaacaata tattgtgtag ttattgttgg acttacatta tgtggaccgg
     1381 tacctgaagc tgtaaatcca cttggattgc ttgtccatga ataagtataa ctcggatcat
     1441 taatactact tacattaact gtagttgttc caccagggca caatgtaggg tctggagtag
     1501 taacactaag tgcaggtgga gcagttactg tggcagtaat aggtacacga actccttcac
     1561 aacctgtaga aatttttaaa ttataaaaat aatattgata atttggtgta agatcataat
     1621 ctagtgatgc ataacccggc aatacccaag gtaaagaatg tgtagcagaa cctgagcgcc
     1681 atgaatttgg gttagtacca acaaacccaa tataataggt acctattggt aatacccaat
     1741 ttacattaac cgtttgagcc actgtgggtg taccggagtt tacaacggtt gtatttcccg
     1801 aatagctttc aaacattggg cctgaagcac tagttgttct tgcttctatg ccgaatgctg
     1861 tacctaaagc agcagaagga taaatgtcaa aagattctaa agtgaatggt tgggtaaccg
     1921 ttaaaatcat aaaggaacca gtgattaagg tcgtctgata gctacctgat gaagtgacat
     1981 gatcccagtc accatcaccg gggatggtta gtccatttgc tccaccagaa cttggtgaaa
     2041 catagtaagt tgtagtagta ttaagattgg gagtagtata tgtggttcct gttgcaatag
     2101 gtgtacctcc agatgagttg gtgtaccaat ttcctgttgt acccatatta gcattaatac
     2161 ctaaagtaac aggtccataa ccacaacgtg ttccgggaac taaactatta atctgtggat
     2221 tatttaccgt tacagcaacc acattggatg ttgcggtgtt actattacaa accgtcaatg
     2281 cttgatacca tgtactttga gttacagtgc cgggagtata agtagaatca ttagttccta
     2341 cgtttgtcca gggaccagta gcgctgacag ttgactgttg ccattgaata tcgccaccat
     2401 aatgtccggt aagtgataaa acaggatcac cactaacaca tagacttgaa accgatatac
     2461 tggcagttcc agctattgga cctactgtta tactgatggc aggtaaagcg tcattggtat
     2521 tatcgccatc gctaaccatt gaagttgatg cattcaacgt ataggtaccg tttgctgttc
     2581 catctactgt tggtgataat gaaacgttca tggttgcacc ggaagctaat gttcctgaag
     2641 acaatgttgc actaactgtt gtattgaccg gaccggtaat cacacaatta acagtaaccg
     2701 ggtctataga gaaatctaag ttctgaacac caaaattctt gatttctacc acaatggttt
     2761 cagcattggt aaaacatcca tcagggttag gtccaatgaa agcagaaacg ccaatgtctt
     2821 tatttgctgc tgcaaactcg taagcaccgg ggtcaggagt tgtcatgtta cgcaatgcac
     2881 cggtgatatc atctgttaca cctaaagctg caccaatatt atccaatgcc acattagtag
     2941 gtttgtaatt ctctaacgca atattggcaa aattaggatt cacgctttga ctgttctgat
     3001 cccaggcacc accgttggca gcttgccagt tggcaaatgt tgcatgtgca ccggaagaat
     3061 agccaatatt attgctaccg gatggtgcat taatatacaa atcattatta ttactagcta
     3121 ctgcagtatt tgtgccaaaa tacagacaat acttggttcc tgtaccacca cgtgttatag
     3181 atatgatgtt gttcttaact tctgttgatg ttccgtaggc ataaatacca taggttgagg
     3241 ttgtgcctgt tgcaccggca tcatcaaatg aaatagtatt gtgatagata tgtacatagt
     3301 tatatgatgg gcaatacaga ccatacaaag ctccgcttga ctcaatgtca tatactaaat
     3361 tattaatcca tttattttca aaaccggcag caccactggc tgtattatac atgcaatatg
     3421 cagttgaagt acttgaaggt atagcatcaa acagttttct gattctgttc ttttccacta
     3481 aaatattata ggaacttgtg ctcatataaa taccataagt tgtgctggtg ctggctctgt
     3541 ttgggcgttc aatgatattg ttacttacaa tggtatttct acaataggag tgatagatac
     3601 cataaaaata aaaatcgcga atggtgctgt ttataatttg attatctaaa ttataagggg
     3661 atgtggttaa accggtaatc actgtgccat aataccctcc aatcatttca caatcactaa
     3721 acacgttacc actaccggac aatccggtgg ttgtacttgt agttgcactg ccatttacaa
     3781 ctaatgctgc aagaacagta gttgttcctg taatagatgt agtgaatgta catttagaaa
     3841 atgtgttgct gtctgctcct ccataaaggt gacatgcaat gccataagta ccattggtgg
     3901 cttcaatatt caaactatcg aaagtaatat aatctgcacc atttaatgcc aaagtatttg
     3961 gattactcac aacaccgtta aaagataaag tattaccgtt tccattaaac gtaatggtgt
     4021 ttactgatga agcaccactg atttccggaa taactacctg ttcgttgtat ggacctgaac
     4081 ctgcaacaac attaaataca acaggacctg taattccaca actcaaagcc tgtatagcat
     4141 catcaaaagt ctgaaagtta cttccacttg ttggcaaggc actattgatg gtataagttc
     4201 cagaaggtgc cagacataaa taatatgagt cagtgttatt tccggatgta gcatcagctc
     4261 caccatttac ggccaatata tttaccgtca tggttacact gctgactaaa ttgtattgtg
     4321 ttgtaaacga caggttggtg ttattgcctg aggtaagtcc taatccggta aatgtttgca
     4381 ctaccggagc accgccatca ctataggaaa gttgaatgct attgatggtt tgagcctgcg
     4441 tgttatcaat atttacagtt atggttttta atcctggact tgatggactt gttggagtaa
     4501 accaggttat tcctgcatcc aatccaccca atgaaaattc ataagcaccc atatcaggtg
     4561 aagtgagaga acgtggtgca ccattgatat catcagtaac acctactgga gttccggcat
     4621 cgtttaccaa gccattgctt ggcgtataat cgtaagtgcc gggggcaaca tagagtggat
     4681 ctacacttac actattagcg tctttgccac tgcccgactg ccatgctgcc aatgttgtat
     4741 atgccgtacc attataccca atattattag tacctgctga agcattcata tacaagtcat
     4801 tataatcgct tgtagctgct gaaggattgg aatagtaaac atcatacttt gttccggttc
     4861 cacctcggct aatgctcaca atattatttc taacatcata agctaatgac cccgtacagt
     4921 agaatccgta agttgtaccg gtagctgttg aagatgcatc gtccaatgaa atagtattat
     4981 gataagcttt ccagtagtca ctgccactca tataaaatcc ataaactgtt ccattagaat
     5041 taatatcaga aatcacgttg ttagaaaatt tattttcatt tccaagcgtt gcatcaatgg
     5101 tacaatatat agaatattga atactggtac ttgtcgggtt tgttgcaaat gtatttctga
     5161 ttttgtttct ttcaactaat gcattggtat ttccggtgga aagataaatt ccataatagg
     5221 tacttaggtt agttctggta ggtcgtgata aggtgttgcc tctgaacaca agtccgtttt
     5281 gatataaaaa gtatgtagca taattatatt gatcaagtat attacaattg attacctgat
     5341 tgccggtact aggcaaacta ctattaccaa ctattgcagt attataataa ccactaaaca
     5401 tggtacaacc agtaattaca ttgtttattc cactattacc tgaggcagta ccagatgtgg
     5461 ccgaaccact gattgagaat ggcaccattg aagtacttgt accatttaca ggtgcattaa
     5521 aggtacaatt gctaaaggta ttgttgtcgg cattgttcca taagtgacac accaaggcat
     5581 aggttgtgcc tgtgccttct actgtcagat tatttaccgt catataatcc gtaccattta
     5641 ataccaaagt gccgggggct gtgcttacag tagctccata agttaaaacg ttgccattac
     5701 cattaatagt tattgtatta actgatgaaa cacctgtaat ttcaccgaac tcaacttgtt
     5761 cgttataggg accactgccg gcaacaacat tcacaacaat agctcctgca atgccgcaat
     5821 tgagtgcatt tttaaaatcg ttgaaagtct gaaagtttga cccactggta ggtagtgcag
     5881 aattgatggt atagtttcct cccggaaaac tggggtttac agtaactaat acaggagtac
     5941 ttagttgact attgccactg caagttactt cgcactgata gtatttggta gttgaagcca
     6001 ggatattgag aacagaagaa ttagacgatg ctccaatatt tgtgtagggc cctccaattg
     6061 tggttgattc ctgccattga taagtttgtc ctgagcctac gctgttgccg gttaatgaaa
     6121 gaaccacatt atttcctaaa caaagtccgg aagaaggtgc tgctgaggca gaacccgggg
     6181 ttggcggaac tgtacagggg ccactagtaa aggttatttg aatattgggt ctgtttttat
     6241 aatcagctac agctccagta agtgtggctg gatatgaact gcctgttgct caaacaatta
     6301 agcttgcact gctgccggaa gtaccagaat attcccacaa aaatttatca gtagcaccac
     6361 cattacctgt catctgcatt tcgtatgcaa tttccagtgc acctccagta taattaaacg
     6421 gtgtggttaa aacccaatca acccagccta tagtatttgg aatattgaat gatgaacttt
     6481 gaaatacttg attatgagta tttaaaatac tagtccatgc taaacctgaa gcaagagatg
     6541 tgttggatgt atttgccatc aacatattat aattacttgc aggggtcaca aaatttgctg
     6601 tgttgccttt cttaaaagca atctttgtta ttgttgcacc tgcaggtatt cctgcggcac
     6661 tcatttctgc agctgtgtat aaaatattcc ctctgcaatg ggtggttgtg cttgtggcac
     6721 taaacctata aacagggctg taaagcgtgt ttgaagggag tgtgtttcca gttcctatct
     6781 gaaccgttgt ttgagcctgc acattaattg caaacagtaa tgcaattaat aaactcattg
     6841 ctacccttat taaatttcct aaaggatgtt tgttttggta atttgaagaa atcatttgtt
     6901 tggattttta gttatataaa tttatttttt tgaaattgaa ttccatctgc gacaatcaaa
     6961 tataaaatct aaagtattta aagagttata ttcaatagct aaaacacact actacttttt
     7021 agcatgtaca aatttcattt gtaaattatt tattctttcg gttttaatca ttatacactc
     7081 agacagtcat agactgtact actatcgcaa aacaaattaa ataaaaaaga aataatttac
     7141 atgtagggat agaactacaa gttgagtttt ccttctaaac tactcataat taactgcatt
     7201 attttaaatt tttttattta aaattcagaa cttgtgacaa tcgctctaca caaataaatt
     7261 atttatgtat ttttaacgtg ctaaaatcct acttgtaaaa tgaatgcatt tgctgactta
     7321 tcaatcctta tagctgacga tcacgttatt attagacgtg gattaaaatt tttactggaa
     7381 acacatttcg gaaaaatcag tattgttgaa accgattcat caaaaggcat tttatcatct
     7441 ttggccaaac atcagtttac acatctcatt ttagacatgc aattagcaga tgggaattta
     7501 cttgaaattt ttcaggaaat aaaacagcaa aatccacatc tgcccatatt gatatactcc
     7561 atgagtccgg aagatatctt tgccaaacga ctgaacaatc ttggtgcatc gggatatctg
     7621 aacaaacaaa gcagcgaaga agaagtagtg aaagcaatgg aactcttctt taccggcaga
     7681 aaatatgtca gtcaaaaata ttttgacatg cagcatagtg ccacaaaagg aaaaagcgac
     7741 aatccgtttt caaatctgtc ggaaagagaa acagaagttc tgctactcct gttgaaagga
     7801 aaaagtgtaa aggagatagc ttcttacttt gatttaaaat caaacacggt tgctaccttt
     7861 aaagccagaa tatttgataa aataggtgta aataacttaa tggatttaca aaactcagca
     7921 cgggcatata acattccaac agtttgagtt atatgcaaaa attgcgagtt cttttaatta
     7981 tcttattatt ttctttttcc aaattaccag cacagaacag ctatagtatc caaaattaca
     8041 ccattaaaaa aggacttccg gacaataata tacttggatt gttttctgag aaaaatggat
     8101 atgtttgggt tgtttatgga agtggcttag cccgttttga cggtattaac tttaaaaaat
     8161 attttacttc agaaacacct tatctcaact tattcatttc taaatctgcc aatggtgata
     8221 aatttatgat tgagccaagt ggaaatattt ttcaggttaa agatttatca attgacacga
     8281 taagaaaagg aaaaatcaat tcgcttaatt atattaatat caaaggaaca cttcctgact
     8341 taaaaacata tctggaacta tctacacctc atgtaaacat aacagttgac aaaaattgga
     8401 tattaacccc tttaattatg ttccctttaa aagacagctc ttacattgtc cgaacaaaaa
     8461 aaggaattgc acatattaaa ggcaaaaaaa atatcaagga acttaattta gagcatttgc
     8521 ataaccgaaa gtatatttca gcaaatggga atatttatgt ttttgtgaat gactccgaaa
     8581 tatacagagt tgatgtagca tcgtggtcat tggttagatg caaattaaat ggtgacctga
     8641 attcatataa aaatttttcc agcaaaatca ccaacacatt ctggaatttt aacaatagcg
     8701 acccatgtgt tcaggttgat tttaacttgt attcattttt accggactca ttaaatgtaa
     8761 atgagataaa caccattcaa ttgactaacc aacttcctaa taactgtctg attacctcca
     8821 ttatttataa tgcagatcag aatttgatat taacaggaac agatacacgc ggattgtttg
     8881 tttataagaa acgcaatttt aaatccttag ccttctcgaa ccccgaaccc ggcaccaaca
     8941 acacttattt ctgtcaaata gcattaaatg acagcactgt atataccgat tggaacagag
     9001 aatttaccat taatggaggc aaaccaagca agctccacct tacaaggaat tattccgaaa
     9061 atatttacat tgacaaaaaa ggtgactact ggtatgctga aaacaacaca ttggttcgtt
     9121 ataatccaaa aaccaaagca aaaaagtaca tttattatac cggagacgaa ttgccacttt
     9181 cgttttatga agaaggtgat tctttgtggg ttgggcttat taaaagtttt ggctgtgtta
     9241 ttgatgacac attaaagcat cttaaaatat tagacaatga agaatcaaac tctaatattt
     9301 ttcagatgct gcgttatgaa ggtaagcttt ggatatgtaa ctataccggg gtttacgctt
     9361 acaatactca gaatggcaag ttagatactt tacatgatct tgctcttaaa tatacgtatc
     9421 actttaaggt gataaacgat tatcttctga ttggcacgta tggtcgtggc tattatatgt
     9481 ataaaaatgg caagactgta aaagtgccaa acggcagatt tgaagcatta aaacaggtac
     9541 ataattttgt acttgattca ttagggtatc tttggatggc caccaacaat ggcgtattca
     9601 aaacaaaatt cgaggaatta gttcactact ttaatgatgc cacctatcag atttcttatt
     9661 ttcactattc agaagaagat ggtatcatca accctgaatt taatggtggc tgcgacccaa
     9721 attatttaag acttaaaaac ggatatgttt cattgcctaa tgtagaagga ttggtttggt
     9781 tttatcctga aaaaattgaa gacccacggt ctgattaccc aataagcatt gatcaattta
     9841 ttgcagatga ttcaatttac aattctttaa attctatcct tgttccggag tctgttcaat
     9901 caatcagagt agaattttca aacccttatt gggggcaacc tgagaatatt ttcagtgaat
     9961 ataaattaga aggctataac aaactttggg tcaatttatt acctgatcaa tctgccatcg
    10021 agttttcaaa tcttaaaaca ggaaaataca agctgcatat acgcaagcaa acaggacttt
    10081 ctaaagatta tactgaagcg acaattgcat ttcagattga aaaaaaatat tatgaaacaa
    10141 tatggttttg gttggctatg accggacttg cacttttaat catcataatt gttgcaagac
    10201 tttattcata taatatcaga cgaaaaaata tattacttga aaaaaacgta gtcttgcgca
    10261 catcagaact gcaaatagcc tataatgaat tatcagaatc aattaaaatc aaagacaaac
    10321 taatctcgat aatatcacat gatctgataa caccattaag atttatcact atggttgcaa
    10381 aaaaaggtgc cggagaagat gttgaaatgg ataccaaaaa acataaagaa ttattaggag
    10441 aaatagaatt cactacagat aaacttcgcg caaatgctga aaatatacta aactggatta
    10501 agcaacagaa taaacgtata gaaagacaaa ttaccagtgt ggcaatcggt gcattagcag
    10561 aagatattac tgatcaattt atgcacatta cttataataa aggcatcaga cttgtcagca
    10621 ctatttctca tgatgatatt attcaatccg attcaaattt actgtcaata attttacaca
    10681 acataatttc caatgcaact aaatttactg aaaatggttt tataaaaatt tcttcaaggc
    10741 aaaacagtca ctatgaaatt acaatagagg acaacggcaa aggtatgtct gaaaagcagt
    10801 taacacgact atctaaactg ataaacaagc aaccattaaa tgaggaagat gaacaatctg
    10861 ataacaaaga aggatttggg ctcggttatg ttattatttc tgaacttatc caaatcctca
    10921 acggaaaact tgaagtgcaa agcaaattag gtgcaggcac aaaagttaca atcagccttc
    10981 ccttaattta tcattgattt ttttgtttaa gcaccactcc attccaatgc aaattgtcag
    11041 atgtaacaca taaataataa attccgttac tcaaaaatga aacatctaaa gtaatatcat
    11101 cttcatcagg aaaaactttt tttacccatt ttccgacttc tttacctgct acatctttta
    11161 aaataattaa aaccgttaaa gatgattctt tcagcaacat tctaagatgc atttcattgc
    11221 cgattggatt cggataaaca gaaataagtc tatcgtgact gtactcattt gccactttat
    11281 tctgtgcaac cgaattttgt gcaacctgaa taacatagga tttaataagt ttagcaacct
    11341 caacactttc atcacaattg ctccggcttt taacacccac atatccacct gtaaaagcag
    11401 gaaggaaatc aacggtaatg gcattgccga actggccgga tgcaactgta gcatcaccag
    11461 taaccgtcca gaaataatct gttgctccac tgaccggaac aatgctataa gaaacaccag
    11521 agcttccacc cagaacagca gcaggacctg aaataacacc gggaacagga ggctttgtgc
    11581 ttagctcaat acaactaaca gcattattct tcagacaagc ttctttgctt gcaacacaaa
    11641 ttcttccata gacaaaatta ctgtcaacag aggcatttac ctgcaaagta ttaaaagtat
    11701 ttacaatacc agattggcca ttcaaaaaaa tattatctgt agcttgccac caatacgatg
    11761 aaaggctact aaaagcattt acagaacaaa caaaagtatc gcccgggcaa acaacactat
    11821 ttcctgttaa actaaccgga aaagatttaa ctgaagtaag actgaccaca gaaggattac
    11881 tgcttccgca aatatttaat gcactgacag aaatattgta ctgaggaaga acagcattta
    11941 tatcaaagtt aactaaggtg tctgtatttg aagaagtaaa atctaccccg gcagaagcag
    12001 tccaaatgta actatctgca tcaggtaatg attgaataga caacacttca ttacttgacg
    12061 gagggcaaac caaaactgaa ccaacaatac cggaaggttg tgaaggtact cctcctacta
    12121 cttcaatagt ttttgtatct ataacatgac aaccatacat atcattgtat gtatatgcaa
    12181 ttgtatgtat ccctttcccg gcaacagttg gattgaaaac agtatccacc acgccaaggc
    12241 ctgaaaaaaa tccgccaata ggtgttgcac ttaattgtac gggatcatca gtaacacaaa
    12301 cttttgtaac aggaactgta agttgtggca cgacatcaca ttctttaaca acactaaatg
    12361 cgtcaagcac ctgtggtgca ccaacattgt tgtcaacaaa tgttacagtt agtgtgtctg
    12421 cctctatttc aatgagtaat gaaccaagtt cctgatcata atatcttttc attgcaggat
    12481 gaggccattc cggaccgaca ggatctaaaa cacctgagca tccggcttgc accacaacta
    12541 atcctttata atccttcgaa gactttttca gatagggttg tggcaattga ccataagaag
    12601 agtccacaac catggttgaa tcaaatgttg acgataatcc atagtgtcca tccataaaat
    12661 aagaacgctc atacacatgg ttatgcccac acaataccaa atctacttta tgttgatcca
    12721 gaataggcat tatattttgt cttatcaaaa tcaagtctgt ttcagtatct gaattatgac
    12781 cacctttggt gtatggtgag ttatgaaaca ctacaatttt ccatttctgt tgtgttgctg
    12841 taatatcact ctgtaaccag ttaagcataa cacttcccgt ttgcaataac gaaagtgtat
    12901 ttgtctctaa tgctataaaa tgaatgttgc cataatcgaa ggagtaatat gcttctgaac
    12961 cggaaggcaa accaccacat tctccatttt taggaaaagt gaaaatatca taataaggtc
    13021 cggtttgtgt gattgcatta gctgtataat tttcatgatt acctgcaaca ctaaagacgg
    13081 gtatattttt taaaatatcc tcatagtgat tttgaaaaaa tgcatcctga tactcctgat
    13141 ctgtacctga ataataggcg ttatcaccca acatatacaa caaatcaatt ggcaaggtgt
    13201 cattataatt cagaaatgcg tctcttacat tgttctgatc aatggtgttg atgcctgtat
    13261 cacccataga ccaaatcctg acatgctttg acattccttt tacaggtgct gtcttaaaat
    13321 aattatttga atcgccctga agtatattta ttccatcact gacggcataa taatatcgtg
    13381 tatcaggact aagccccgtt aatcttatct catgatcaat tacgtttaca gaatccgtca
    13441 ttgtaaagtt taatgaatcc ggatcattac cataaagaac tgctgtttga cataaaacat
    13501 tagttctcca acgaattgtt attgctgaat cggcaaccat ctgaagataa ggctgacgta
    13561 acaattgagg cacaaaatta ttgtatgcat ttaattgaag atcgaaagac atatccgaac
    13621 taatattatt gatctgatgc acttcaacgg caataacatt tataccagaa agaaaatctg
    13681 caggcactaa tactattgac tgtaacgcat tgccatcatc aacacaatca gtatatgcag
    13741 atgtactata tcctacattg cctgctgcca tgtttgatct gaaaacttct gtgccattaa
    13801 tataaacaat agcaccatcg tctcttttca aatccagatg atatgaagca aaatcatacg
    13861 gattgtttaa agcaattgtc tttctgaaat aagttgtaat aaatttatta ttcggatcgc
    13921 caccgtaatt gataattgta gtttcatctc catcaccata cccaaactct ccatttccaa
    13981 ttgcccatgc tgaatcatta tacccaatct gactccagtt tgatgcaggc aaataaccac
    14041 tatcaaaata tttccatgaa tcactaaaac taaccaaggt agtatctgcc tttgcaaaaa
    14101 aaggcatgta aaataaaaac atcaataaaa actgctttgt agtcatgtca aaaaaaattg
    14161 cgttaaagac ttaactttca acatctacta agaaacaaaa aaccgactta taaaaagtcg
    14221 gttttcaatt aaagttttac acaattcatc aattactcaa tgacgatttt ctgagtgatg
    14281 cggtgattat atccatctgt aatttgcatc agataaatac ctggcgcatt tccatctcgc
    14341 ttccatccaa tactttcagc atcaggatga aacctatgaa ccatttcacc aagaatattg
    14401 gtcaccgtaa tggttgtaga ggatatttca aatccgttta attttatatt ggttacttct
    14461 ttaaatggat tagggaatac ctctgcagac acctgcattt ccaaatctgt ttcataacct
    14521 tcggaacggc agctaacgaa tttaattgat ttctcggcag aatttctaac gcaaccgtta
    14581 taggtgtgta ccactctgta ttgtccggca atgcttgtct gatacgtttg tgatgtagct
    14641 ccgggaatat ttactttttc atgttgccat tgaattgcaa caccggaata aatattactg
    14701 cttaatgttg cctgttgccc attacataat gttgttcccg gtgttacagt tactgttggc
    14761 gtggtgtttg tctgcacaga aagtattgct gtatttgatg ttacaacatt acaaccggat
    14821 aaatgcactt ttacataata ttgtcctgaa ctagatgcat taaatgaatt attagttgca
    14881 ttgctgattg caacgttgtt tctataccat tgataagttg ctcctgttgt tggtaacaca
    14941 gaaaatgttt gtggtgtacc cgcacaaaca gttgtagttc ctgcaggatt tacagaagca
    15001 ttgatctgca tagcatttac tgttacctga tttgactcta gcgatccaca gctgtttgat
    15061 acatcaacag tataaattcc ggtttcttca gctaagaatg ttgactgtgt agcaccgcta
    15121 atcattatac catctttttt ccattgatat gataaaccat tacctgtatt ggctgataat
    15181 agtataaaag aacccggaca gatagtttgc gttgctgcag gtgtaatact actggaaaga
    15241 gaaattactg taagtgccac tgaaggtgaa gtagaatttc cacatgaact cacaatacta
    15301 accgtataat ttccctgaga ttttgcataa tatgttgatg catttgctcc aactattgta
    15361 ttaccatcta aataccattg ataagaaatg ccactggcag gattggctgt tagcagaaca
    15421 ctatcgccag agcaaatagt ctgtgtagta tcaggactaa ttgttgcaat aatagagcca
    15481 acagaaactt gtaccgatgc agaagtatag acaccacaac tgtttgagat tgtaacagaa
    15541 taatttccgg caacattggc attaaaagtc tggcttattg caccactaat tggatttcca
    15601 ttaaggttcc actgataatt atttccaaag cctgtattag cagtaaagtt tacacttcca
    15661 ccggagcaaa ttatctgagc tgcatcaggt gatattgctg ctgtaacagg cactacatta
    15721 acagtaactg cttgtgatgt ttgtgtacca catccattag ttattgtaac tgagtaaacg
    15781 ccagatacgt tagcggaata agaatcagat atagctccgg gtattgcatt accatcaaca
    15841 ttccattgat aattgttacc gggttcatta gaagttgaaa atagcacatt attcccctga
    15901 catactgttt gtgtagttga aggcgaaata gaaatctgca tagaacttgc tgtaacagca
    15961 acaatattag aaagagctgc acatgtagtt gttattctac attgataatt ccctgatgta
    16021 cttgcaatga aactttgatt tgttgcaccg gcaatatcca ctccatctgc aagccattga
    16081 taggttgcac cagaaacaat atttgcattt aaagtaactg tttgtccatt acataaatta
    16141 acgcttccgt tagcagtaat tgttgctgta ggccctgtaa caaccttaat agcaatggca
    16201 ggtgattttt ttgagcatcc atttgcagtt tgtgtgacac ggtaagttcc ggcaagtgta
    16261 gttgtatgca attgcgaggt tgcaccattc acagcagagc catttctctc ccattgatag
    16321 gatactccgg aaaaagtatt ggttgaaagt attactgaac taccactaca aacatttaat
    16381 gaaccgttag cagaaatagt cggtgtaggg ttattaatta ctgtaacaac aattgtgtta
    16441 gaatttccta cacatcccgg tgcaacaatc cgacaatagt agttgccatg gctagatgca
    16501 ttatatgaag agcctgtagc tccggaaatt gcaccattat ttctatacca ctggaatgta
    16561 aaaccggttc cggtctgagc agaaagcata actttacttc ctgcacatat atccgttgct
    16621 cctaatgcag atataacatg cgaactacct gtagccgtta caacattgga taatgaagca
    16681 catccggttg agtcggtggc taatacagaa taacttcctg cacttaatat ctctaggcta
    16741 ctatttgtag ctccgttaat atcaacacca ttatttttcc attgataagt atagcctaca
    16801 cctgtgtttg cagcaagtgt aaccggacct gagcaaaggt ttacagatcc tgaaggtgaa
    16861 atgctaacag aaatagctgc tccaactatt gttgctgatg aaacaactgt tccacaagat
    16921 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtataggtg taagtaatag
    17041 tatgtgtgcc aataccggca acagatggat cgaaaacacc attatttaca ccaatgccgg
    17101 aatatgttcc accggaagga ctgcccccgg acaaagcaaa tgcactttgt ccggaacaaa
    17161 cattattata tggtgcaagt gtcacttgct gatcacattg tttgatgatg gtaaattgat
    17221 catagattgt tggattaggc aaggtgttgt caataaactt tgcataaagt gtatcgccat
    17281 tgatgtctaa aaccattgaa ccatacgttg aagaagaata agaatacatg gcattgtgtg
    17341 gccagccggg tgaagtacct tcaagtattc ctcctgttcc ggcaactgca taaacagtac
    17401 cctgaaaatt ggaagatgct gatttaatat aaggtgttgc tcctgccccg cttccaccat
    17461 caacaagatt ggaattatta aaagtgcttt cagagccaaa gtggtttttg ataaagaaag
    17521 aacgttcata gttatggcta tgaccggtta gaacaaggtc aactttatat tgctccagaa
    17581 taggaacaaa attttgtcgc atttctatca attcagtttc tgtgtcggag ttatggctac
    17641 ccattgaata tggtgggtga tgaaaataaa caacagtcca tttttgtgta ttagcagcaa
    17701 ggtctgcctg aagccaattc atcattgcac caccaattgt tctgaaagat gctgttgttg
    17761 attcaagtac aatgaagtga atatttgcat agttgtatga ataataggct ttcttacctg
    17821 atggtacacc accggcttct ccgttagtag ggaagttaaa caactcatag taggggcctg
    17881 tttctgtgac tgcattggat gcatacattt catgattacc agctgctggc caaactacag
    17941 tttgacgaag catcttctca tatctgttgt taaacacatt gttctgatac tctgcatcgg
    18001 tgcctgaatt gtaagcattg tctccaagcc acaaccaaag atctgtatat gtattgccgg
    18061 tatagttata ataagcatcg cgaacctgat tctgttccac actgccaaag cccatgtcgc
    18121 ccagcaccca aatacgggta ggttttactg ttcctgcaac tggcggtgtt ataaagtaat
    18181 tttctgcagg gtcattctgt aaaaaattat ttcctgattt aacggcataa taatattttg
    18241 tgtttggttg caagccacta agctgaacaa tatgttctgt aaccggtgat gcactcacgg
    18301 cactatctgt aaaaataccg gaagttgttc catacacaat ttttgacata gcagaaatat
    18361 ctgttctgta tctaacaatg atacttgttg gcgtagcaac attcagatat gagccaatta
    18421 caacagatgg tatagcaggt ggagtgtttc ccataccggt taatgacata tcgaaactca
    18481 aatcagaact gttagcgaca aactgatgaa tctcaactgc aataacgttg gttccattta
    18541 cgaagtaaga cgtagggatg tttgcagtca gtaaagaaga gccatcatcg gctgcatcgt
    18601 tggcggcaag tgtagtgtat gtaacaacac cattaatatt atcgcgccaa acttcaacac
    18661 cgtttacata taccactaca ccatcatcac gcttgatacc taactgcatg gaagtaaagg
    18721 cagaaatgtc agcaatagaa atggtttttc ggaaataggt tgtaataaac ttgttcgaag
    18781 cgttagaacc ataagaaacc actgtgcttt catcaccatc accataacca aattctccgg
    18841 gacctgatgc ccacgttgca tcactaaatg ccacatcacg ccacgctgtt ccctgattgg
    18901 ttccattgtc cagatatttc caaactgaat ttaatggaaa aatagtaaca tttgttggtg
    18961 gtggagtttc taagcctgtg agtcgtaagt caaaacttaa atcagaactg ttaaccacaa
    19021 actgatgtat ttcaacggca ataacattat taccagtgac aaacagagaa gcaggaatat
    19081 tagctgttaa aacagaattt ccatcgtctg cagcatcact ggcagccaaa gtaagatatg
    19141 atatagttcc attgatattg tcgcgccaag cttctacccc gttaacatac acaaccactc
    19201 catcatcacg tcttactcct agctcaaacg ctgtgaaagc tgcaacattg gtaatgttaa
    19261 atgatttacg gaagtaagtt gtaacaaact tgtttgcagc gttaggccca aatgaaacaa
    19321 tagttgattc atctccttcg ccatagccaa acttagcatt accgctactc catgacgcat
    19381 cgctaaaatt agaatcgcgc caggctgtac cctgattcgt tccattgtct aaatatttcc
    19441 aaacagaatt aagaggaaaa attgtttgat cggcattggc taaacatacc attgccatga
    19501 acaaaattgt caatctaagt ttcatcgtaa tcttaaaatt tttaagcgtc acaaaattac
    19561 cctaactaca ctgaaaaagt gtgcgttttt gggtgaatag ttacacacaa tcagagtact
    19621 tctgctactg taaatgtact acctccgaca aaaatcaaat cttgtgctgt tgctgctttt
    19681 tttgcactta gcaacgcatg tttaaccgat gtataatatt caccttgcaa tcccacttct
    19741 atagccttgc ttctcaattc tttagcaggc attgcacgag ggattttagc attgcaaaaa
    19801 tagcagattg catgctttgg caatagcttc agcacaggca taacgtcctt atcattaacc
    19861 ataccaataa ccaaatgaag ctttttaaaa ctaatcttac aaatttggcg aattacagtt
    19921 ttaatgccgt caacgttgtg tccggtgtct gctataagga gcggtttagt tcccaatacc
    19981 tgccatctgc ctctgaatcc ggtcagatgt tgaacttttg caagtgcctt ttttacattt
    20041 ttcttttcaa ttgcaaaacc tgaatctttc aataactcaa ttactgacaa aacagttgcc
    20101 acatttttta attgataatc accggcaaga cttaatttca aattattata aatttcaccg
    20161 tgctttaatt gagcatcaag caacaaatat tccagatgtt tttttttaag ccggacttta
    20221 cactgactgc ttgcaaaata aattggtgct gcattttcaa tagctttttt cagaaaaact
    20281 ttttttgtat gaagcgttgt ttcaccaatc agaacaggaa tattcttttt tattattccc
    20341 gctttttcaa ctgcaatttt ttcaagcgta tcgcctaaaa gattcacatg atcccaaccg
    20401 atgtttgtaa ttacagaaag ttgtggctga ataatatttg ttgaatccaa tcgtccgcct
    20461 aaaccggttt caattactgc aacatcaact ttacaatgcc ggaaataatc gaatgccaac
    20521 gcaactgtcc attcaaaaaa agatggtttt acaacatcaa attcattcat atattgttgt
    20581 acaaaaccgc ttacatactt tttaggaatc atctttccat taactcttat ccgctctctg
    20641 aaatctttca gatgaggtga ggtaaacaat cctgtcttaa aaccttgttg ttgcaatacg
    20701 gcagcaagca tatttgaggt tgatccttta ccattggtgc cggcaatatg aatagattta
    20761 aaagatttat gtggatgatt gagtatgttg tctaatgcaa tactgttatc taaattattt
    20821 ttataagctg ctgcaccaat gcgatgaaac attggcaact tagaaaacaa ataattcagt
    20881 gtttcggtat agttcatttt actgaacaag aaaaacaaag gtaatggttc ccctttgctc
    20941 ttcaacccct tgtggacttt gattaaattt cgccttaaat gcagcgtctt ttgcttttct
    21001 aaaaaggttg gagcttgtgg tagttgaacc tctggcaccg ggaactgcat caattacgtt
    21061 gccatactta tcaactttta ttgacaccac aaccttacct gtttcctggc tattatcata
    21121 aattgttggc ttggcacgaa cagaccttcc tgctaaatca taagaaaatc cggggcctga
    21181 gccactaccc tttccgggtc ctgaaccatc accttctcca cctccactgc cacttccacc
    21241 ttgtccggtg cctttaccct gatataatgc attaggatcg ccatcacgag tgccttgatc
    21301 accactgcct gtgttagatg ttccctgcga agcagattta tttttcttgc caggatataa
    21361 agcctctgca tttactttag gaacaacagg ctctactttt ttaacttcag tttttacagg
    21421 tgtttcaacc gttttaataa cttttttttc cggagtcttt tcttttttag cttcaacaac
    21481 agcactctct tctgtttcct gtgtggtata attttgttcc tgaactacag cagggggtgt
    21541 tgctgttgca ggatgtggat tctcggaaac attttctgat tccggttgaa tatctcctgt
    21601 tgacaattct acaaaaccaa gattagcaga ttctgcacca ccaccaccac caaagggtgg
    21661 aatttttgtg tgcatggtga accaccataa aagaaacaac aagatgccat gcagcaccaa
    21721 ggttaaaagt aaagagtttc tttttatagc cggagcttcc atgttatttc actgtgttgg
    21781 ttgcaagcac catttttatt ttattgcggg caccaatatc aagcaagtca accaagcctt
    21841 gcacctgaac acttttatca actttcaaaa ttgctgttgg atcttcctgg ccttgcaatt
    21901 gggcaagaat agcattttcc aattgatcaa acactaccac ctgattgttc actgcatact
    21961 gcatatcggc agtaactgaa attgtcaccg ggtgcttcac agcaggcttg ctggtttttg
    22021 cattcggcaa caacagtttt aaaacgtttg gtgtagccat tgttgatgta atcaggaaaa
    22081 acaataaaag gaaaaacata atatcattga gggagccaac aaaaacttcc cctgtcattt
    22141 tatttcgcct tctcagattc aatgccataa tgatgctttg tttaacagga tttcacaatt
    22201 atttttttac aggctcttcg agcaggtcaa taaactcaac agcattaatt tccattttgt
    22261 ttactgcacg gtcaactaac gatatcagat aatgataacc tgcataagct gcaacaccaa
    22321 ttaataaacc tgcggcactg gttatcattt ttacatacag accacctgaa atggtgtcaa
    22381 tctgcaaact attttccaat gatatctgat agaaaatttt tattactccg aagatggttc
    22441 cgaggaaacc aaacatgggc gcaattcctg caattgttcc caagaaactc aagttctttt
    22501 caagtttata aatttcgagt ttacccacat tctctattga tgattctata tcacggatag
    22561 gtttacccaa gcgcagcaac cctttttcaa tcatacgtgc cactggatgg tttgtgtttc
    22621 tacacaaggc ctttgctgca tcaacattac ctgtatggac aaagtctttt atctgattga
    22681 taaaattctt atcaatattg gacgactttc tgatggtaaa gtatctctcg aaaaatatat
    22741 aaacagcagc aacagacaac aatcctaacg gaatcattat cggtcctccc ttgataacca
    22801 aatcaagaaa cgaaattgat tgttcggcct tcaccgcagg ctgtaatgca gtggcgctat
    22861 ccgtaacagc ttgcgtaatt tgcaataaaa tgctcatgtt gaattcgaaa ttttagaaca
    22921 gtaaaagtac acccatgccc ctataaaata atcaagtgtc actgctattt taaacacagt
    22981 tttgttgata aggcaaacgg aaataaaagc aaaaaagtat aagttgttta tcctaaaatt
    23041 gagatgaaag gaagattatt gtccgtttaa ttgataatca gtttacctga atttatcaac
    23101 ttaactcctc tgatttgata gaaatagatg cctttattca actttcctaa agaaatcata
    23161 ccggaattgc cctgttgcag atttaattgc attacctgct tacctgcaac atcaaaaagc
    23221 tcgaattgat aattattctc aagggtatga ttaaggcaga taaacacatg ttcagatgcg
    23281 ggattgggat ataactgcac tgtcataccc tgttctgttt cattcaaacc aagtacaccg
    23341 ccattatcgg taaatcgttt tacaaattcc tgataatatt gattggcaat gtttctgctg
    23401 tgtatgatta aagtgttttc atcgttcttc tgattggcag ttgtactcca gttatgcgag
    23461 cctgtcagta ccaaagggtc acttgaagga tcattctgat caacaatcac gtatttatgg
    23521 tgcaacaatc cggtttgtgt tgcatgattg taaagcatca gttttgaacc cataacagca
    23581 tttaaaatac tataggcctg cccaccacca ctaccggtgt catcaaccat accgtatgca
    23641 tctacaccat gatttacaat acggtcttcc gcagcatagg ctaagtcaaa acgtgtaaac
    23701 accaatagtg caaattgcat ttgctgattt gcagtttcaa ttgttgtcat tatttgattg
    23761 tttacattat cagaagggct gaaataagat tccacacgat tgcctccaat attgtattca
    23821 tgcggagtat tgtctttttt atcaggtcca aattttgaat tggaaggatt tggttgtagt
    23881 gtgctgctgc cccacatttc ttcaaactcc attgtgtagc caatggcaag agatttatcc
    23941 tgtataatca ataaattgtt tgcatcatta ttgagttgag cattggtgaa attggttgag
    24001 ccggaaatca caattggata gtttgcattg gaagcaaaag catcaataac cacaaactta
    24061 ttatgcataa tggaataata acccggagga gtagttggag aaggcaacat atttattccg
    24121 ggatttaact gtgctaatcc gctgtttaca ttacctccat cataaataat ccttaccgca
    24181 acacctctgt tgtcggcatc attaatagcc tgaacaattt gtgatgtacc actattctca
    24241 aaattataaa ttgcaatatc aacactctgt tgcgagcggt tgatataggc agcaatggta
    24301 tcctgaaaag ttccgggcag ttgaattgca ttgtttgccg gagtccatgc ataattattg
    24361 tttaccgact tattgaaata aactttcata tcaccgctag aaagtgaagc agtcatgtag
    24421 attttcacag tagcttttgc tgtatcgttt ccatttactg aaaatgcttt tacataatat
    24481 agctgcgaag gagatgcgcc tgcaagtgtt actgtgtgat tagtactacc tgatgtacca
    24541 ttcagaattc ctaattcaag agcaggtgta agtccatatt caataaatga agatgcaggt
    24601 aagttggtag tccagtttac atcaaaacct gacgcgctga tattttgctg aacaggtggt
    24661 gaggtcatgt aaaaagagga tgaaataaaa atatcattct gatcacgagg caataactga
    24721 tatactgttt ggtactgact gcaaactgca atcagactta cagcaccgga aggaattggc
    24781 tgacctacga gattggtatt tgctgcaatg cgcacctgcc cgttttcacc atttgaagta
    24841 aatgcatagt tagcattacc cataaaagtt ccgccaccgt tgttgaacac aactccgtca
    24901 atacgtacta aatgactttc tgtagtctca tcgagcagca gcggtgttgt aacaattggt
    24961 gcaggcaggt tattaccccc tcccagattc tgaaatgcgg tcagcgtagt aatttcaaga
    25021 aggccaaaat aatctgaaag tacacctgtc accaatatag catcaccacg aactgttgca
    25081 tttaattgcg tgctgaacac agcaatacta ccagtaccgt cattgatgta acggatattt
    25141 cctaattcct gtccattgac acaaacacct gtaactgtta cttgcgctcc tgcaggtgag
    25201 gttctggctg tggcaataga tactaactgt gcgaagccat tttgcaacag gagcagaaaa
    25261 aaaacaatac taattaaacg tttcattgga gtacgattga aataaaaaac gcagtgcagg
    25321 aatatcctgc actgcgctaa aaatatttaa tttctattgt atatcactta aatttctaat
    25381 ttgaaattgt tttgtggtgc tgaattgtgt caaaatgcct gtaatgctta ccggagttgt
    25441 tggtgtagct gaacttgcaa aagatgcagc gctacgacaa tagagaacta ctgtatttgg
    25501 tgtgctgcca tcattaatag tcaggctgcc accattggca ccataagtag ttgctgttcc
    25561 attattaatg gtaacattat ttactttaat aagcattgat tcatactgtt caaaatttgc
    25621 tgttgcagtt gccaaatcaa taactgtcgg tgtgattggt gctaatcctg tgattgaaag
    25681 accacctgcc atattattta tttgcagcca tccattaaac tctgctaatg ttaaaccacc
    25741 aacattaaaa tcatattgag agttcattgc aaagtttggt gcagtgctat aacgcaagca
    25801 aatgccaccg gttgcatctt gtacgataac atttctggta tcaagtgcag aggtagtgta
    25861 atcagaaatt acagttgctt taatatgaaa acctccggga actgttgttg taccacttga
    25921 aaataatgtt cttatactgt ctattgaaac ataaacaggt gtacaaacaa caccatcaca
    25981 acgttcgata tttgcaggca catcattcat atcgcgaaga aatacctgag gcgtagcatt
    26041 atacttctgg taaatggcag tgattgatcc ccctttgcat gggataagtg tagtcgcaaa
    26101 attggcatag ccgcttgttc tcacaattaa tgaatgtcca aaacaatcct gaatattgcg
    26161 gttaacagtt gatttatttg cagcatcgcc aaatggcaag ccaacttctg tagaagcaaa
    26221 tgcaacacta tcgaattgaa caagtgtatt taagtcggca tctgtcaatg cattcaactt
    26281 tctctttgca ggaacaacag tcaatcccca tttacctcct acaatataat ttccgatttc
    26341 tgcagcagga atttcagcta ctgctccggc atttaataca ccaattttag gtgtaccatt
    26401 tacagcctga atatataagc cttttgcctt aacaaaaact cttcttccga taaataaaga
    26461 ggtgaagtaa gacggttgat cgagtaatat actaatacct gcagttgtat cctgaacaat
    26521 catctcctta taataattac cggatttgtc atcggccaca ataatggcag agaaagttaa
    26581 atcagaagtt atctgaaccg gaccggttgc attagctaac gttacaacac tcgatactgt
    26641 tgcattggta acaatatccg gatcaacatt agaagaaggt gcattgtcaa aatcatcctt
    26701 tttaacgcag gaaacagtac ttaaagtaat tgcaagaatt gcaaatgctg ttgtgatttt
    26761 aatattcttt atcattgttt tattatattt ctaattagtt attaattcat tcttaaaata
    26821 aagctgatca tataagttct gccaaaagca taactatatt ttgaagcaaa cacatcaggt
    26881 cttcctgaaa gcacagcaga tttatccaat ctgccttgct cgtacccgtt ggtaataatg
    26941 tctttattat cgagcaggtt attgatgtta agattaataa ggaagaaagt gtttctcttt
    27001 aaagatttga tatgattgtt cagtttaatt gaccatcctg cactcaagtc aagtgtcatt
    27061 tgtgcatcgt agcgctcctg tccaagaatt ttttcccatg cctgactgtt ttcatccaca
    27121 ccatcaatga tgtcaccggt gcgtcttgca ggacttgctt caacataaga gtttgagaaa
    27181 caattggcat taacaccaac ataccagaat tttttagaac gataattcaa tccaactgta
    27241 aatgcatttt gaggtgtgtt gtcaacatgc aggtttttca tatacaccac ttcgcctgtt
    27301 gattgaacag tgtttgtatt atcaacagtt atagttgctg tagggcgtga actataataa
    27361 tattgaccaa ttgaagctac tgccattgca gagagtccca tacccagatt agcatcaaca
    27421 gaaatctctg ttcctgcatg tactttttct atgcctgaca gagtgtagtt tacatagctg
    27481 tttgtgccga atccctgcag ataatacgaa cgactttcaa ttccatctct gaagtttgta
    27541 tagtacaatt taagtttacc tttaatctta ggcgcaatat gtaaatagcc tccttctaaa
    27601 gacgtaattt tttcacttgt aagatcagct actgccgtgt ttcttgagcg tggtgataaa
    27661 tacatgttat taaaaaatgg cgctcttgtc atatagcctg cattagcaaa aacatagttg
    27721 cggccattta ctttataagt agcacctgct ttcacaccaa catcaggaaa tacgtaagtt
    27781 tttgatttgc cataagagtc atccggaaac aagccatttc tataattacc ttctctgtaa
    27841 acagaggtat aagcagcctg tatagcaccg aatatgtcaa tgttattata tttggcaaca
    27901 gcctgtgtcc atgcttttgc atttgtaatg tgtgcctcgt agtcgtagcc aaagcggtca
    27961 ccttcgcgaa ctattctgtt tggatgattt aaatcactct gacttaattg ctcatcctga
    28021 aaatcctgat cagcaaattt atctatgtca acaaaaaatt ctgcacctaa caaatcttct
    28081 gcttctttat aaaagcgtga agtttgtttc tgaaaagaca aacctgtgct tgtagtaata
    28141 ttatcattta aaatagtatt aaatgtggta ttaaaactaa ggcgatttgt ttgcattaca
    28201 cgattttcaa tcaaatacaa tgcttgctta ccgctaacag ttgatcctgc aacaccatcg
    28261 gcattgttaa ccgtctgaaa attctgatcg ttggcaagca acatattatc ccaattgatt
    28321 tgcaataaat caggatttgc tttcagtgta ttgtacactt gtgctgctgc agcagaatct
    28381 gtttgccatg aaggcaaatt tctgtagtat gttgggcgtg ggtcagcagc aaaccaattt
    28441 agtgctgttg attcgcgctt acctgttgta tatgataaag cagaaaacca ggttgtgtta
    28501 ttactaattt tatactcatg atttaatgta aacaccggct gatggcttct tccaatatta
    28561 gcattacgga tttctccgtt ctgatatccc cagtttgaat tataataatg atcgcttgct
    28621 atatcataaa cttctttagt atttgatgat gaacgggcac tttcgtactg actgcctaag
    28681 acggtaaaat caaactcatg cttattgatg actttagaaa cacctaaata ataagcaaaa
    28741 ccgtcataat aggttccttt aataaaacct tctttagccc atctttttga aatagaagct
    28801 gaataagccc atcctttttt gttaaaacca cttccataag ttaacataaa acgattgtca
    28861 tacgttctgt tgcttaatga ataggtagcc tgaatttgtt tacgctgtct tccggcacgt
    28921 gagtcaatac tgtatgaacc gccaacacca ccaaaggcat aagttgatgg attcaaacct
    28981 gaagttgact ctcttgatct tagcacatca ttcaaacctg accagcttga aaatgcagta
    29041 cgaccgttgg ttaaatcttc ttcagcaatt ccgttcatta atgtcgggaa gttttctgtg
    29101 tcatatccac gggttttaaa tcgtgcaaca ctgaaattaa aattagctgc tgaagtaaaa
    29161 gcatcacgag atgcacctaa aacactggat acacttgact gtgcagagtt ggattgcaat
    29221 tcatcatcat caagtgttac aacaggaatg tcggcttctg ttgtagttgt tcccgggctg
    29281 taaaccaatt taaccgaacc aacatcaact gattcagcgt tcacaactac ttcttgttta
    29341 aagccgttgt aagcaacatc ctgtacagaa agtgaataag tgccataagt aacaccactc
    29401 atagtaaatg ctccatcttt gtctgtaatt gattgatagt tggtaccatt cagcacaacc
    29461 acttcacctg ccatcggaac actgttctca tttaaaataa ccccctttat aactccggac
    29521 tgtgcggagg aaagaaaagg aagaatcaat aaagtcatga aaaaactgac tgtagaaaat
    29581 agcttcatat tttataatta gggtggcaaa gctaaataaa aaacaaactt ctgaaaattc
    29641 ataaaacatc tgttaataac ctcctaaaat gtttgttttc aagtgcctaa ccaacatggg
    29701 caataattag gtaaaatctg acattcatgt tttaacatat aaattacaaa ttagtcattt
    29761 taaaatgatt tgaatcacaa tttctgaagt tggtcaataa agagattctt aaaacattat
    29821 ttttgtcgct catttttaaa aatagttaat cgaatggaaa aaattaaagt tttgaatcct
    29881 gttgttgaat tggatggtga tgagatgaca agagttatct ggaagtttat aaaagataaa
    29941 ctcattctac cctacttaga cattgacata aaatattatg accttggcat ggagcatcgc
    30001 gatgctactg atgataaagt aacagtagaa gctgccgaag caataaaaaa atataatgta
    30061 ggaataaaat gtgccacaat tactcctgat gaggcacgtg ttaaagaatt taatctgaaa
    30121 caaatgtgga agtcgccaaa tggaactatc aggaacattc ttgatggaac tgtttttcgt
    30181 gagccaattg tttgtaaaaa tattccacgt ttggtaccaa actggactgc tccaatctgt
    30241 attggtcgtc atgcatttgg tgatcagtat cgtgctacag actttctgac caaaggcaaa
    30301 ggcaaactca ccataaaatt tgaaggcgaa gatggcacta ttgaacatga ggtatttaat
    30361 tttaaaggca atggtgttgc acttgctatg tataacaccg atgaatccat tagaggtttt
    30421 gcacaatcat gttttaatgt tgccctacaa aaaggatggc cattatatct gtcaacaaaa
    30481 aataccattc ttaaaaaata tgatggaaga tttaaagata tttttcagga agtatatgag
    30541 aaggaattta aatcgaagtt tgaagcaaaa aatataacct acgaacatcg tttgattgat
    30601 gacatggttg caagtgcact aaaatggaat ggaaattttg tttgggcatg taaaaattat
    30661 gatggagatg tacagagtga taccgttgcg caaggttttg gctcgcttgg tttgatgact
    30721 tcaacattaa ttacacctga tggaaaaaca atggaagcag aggctgcaca tggaactgta
    30781 acacgtcact tccgcgaaca tcagaaagga aaaccaacat ctacaaatcc tattgcttca
    30841 atatttgcct ggacaagagg actcgatttc agaggtaaat tagacaacaa tgctgcatta
    30901 attaattttg caaaagcgtt agaagaagtt tgtgtagaaa cagtagaaag cggtaaaatg
    30961 actaaagacc tcgctgtatg tatttatggc aatgaaacaa agcctgaaca ttatctgcat
    31021 acagaagatt ttctttcagt gattgacacc aatctgaaga aaaaacttca aaaataaaat
    31081 ggattaaaaa tgcaaaaggg agcttacggg ctcccttttt tatgatatca aattacaatt
    31141 ttactgcatt gaagtattaa tttttgcaaa tgctctacct gtagtagttt cgactttaga
    31201 agcgggtgtg atgattaagt tgttttcatc atcttgttta cactccactc ttattccacg
    31261 agctactttt cccattgcat ccttttcata ggcgg
//
LOCUS       DAOQNP010000027        30872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_462_length_30872_cov_8.345264, whole genome shotgun sequence.
ACCESSION   DAOQNP010000027 DAOQNP010000000
VERSION     DAOQNP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 30872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30872
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_30872_cov_8.345264"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(556..1944)
                     /gene="rlmD"
                     /locus_tag="PKY09_05560"
     CDS             complement(556..1944)
                     /gene="rlmD"
                     /locus_tag="PKY09_05560"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNB12357.1"
                     /translation="MTKRIENLEITDIADEGRGLGRMDGRVVFVNHVVPGDIINADIK
                     RKKNKFIEAAVTEYLKYSDHRTNPSCKHFGVCGGCKRQNMQYEQQLFYKTKQVRDALV
                     RIAGIANPVMEEILPSAQVYQYRNRLDFAFSEKEWLTADKLQNKEYQIQPALGFHITG
                     RFDKVLNIDECLLMQEPTNRIRNFVKSFALKENYTFFNRISQQGDLRNMLVRNTTSGQ
                     WMVVIMFADADEEKRSRMLNAIKEEFPEITSLYYFINQKKNDTFFDLQPVLWSGTETI
                     TESMGGLKFTIGPKTFFQTNPVQANELYKTALSFAALQPQESVYDLYTGTGTIANFLA
                     KQCKRVIGIDNVEDSIVQARLNSESNAIYNTVFFAGDMRETLDASFFEANGFPDVIIT
                     DPPRAGMHPDVVNRICNSGAGRIVYVSCNPSTMARDIALMKDHYTLVKVKPVDMFPQT
                     DHIECVALLEKK"
     gene            2067..4427
                     /locus_tag="PKY09_05565"
     CDS             2067..4427
                     /locus_tag="PKY09_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12358.1"
                     /translation="MPLKHRLLLFLLLMFGFGYEVNAKHIAGGDFTYRRLNGNNFEIT
                     LKIFRDCSDFVAFDNAILIGVFNKGTNTLVNSYTVALTDSGTLTLTGTSCLTPPTGVC
                     MDKCTYVDTISLPNNPSGYYVSWERCCRNNAIVNLTRPGDTGIAYYMEIPDPAIIDNS
                     PNFNNDPFPFMCLGQDFDFDYSAYDVDGDLLTYSLETPLAGELGYPLSSTISPVWSNP
                     TPGPYQQVQWLPGYSVANITGSFPALSLNSSTGLMKVKANKVGFYAMAVVIKEFRNGV
                     QIGQIRREIEFTVINCAANTAPQISYQNSTAPQSGLTFTIYEKDTLCFTVRVTDSDSI
                     KLSYSGDIFAGGSINPPYAVTSAASGNGVATSSFCWYTSCDQGRTQPYSVTFKATDNG
                     CPQPFSSTDSLKIYVLPVPQITAPLIICMNTATEGTIKLTFTDTFAMPRFIDSYVVFR
                     STNGGPYINIGTINNPANHFYNDTTAADLNLNNYCYYIVAVNTCNETGAHSDTICSDT
                     QKNIDAPFIFSSQVQNNNSIVLDFNNPPDNLFTLFKIEKRENSFSAIYADYFQQQGFA
                     IPQWTDSLQNVTEKSYCYRITKKNQCDIESPVSNESCSILLTGQSLPLQHILNWTAYT
                     EWPGGVSYYAIYRRSNNSNTFTEIAQLASNILTFTDTQIPQDNGIFVYYIKAVHDTLN
                     VFSTSNEVTLIQEPSVFTPNSFTPNGDGLNETWSPVSSFIKNYNLKIFNRWGNLIFES
                     NEANRQWDGRYQGQPAPLGVYFYVVTYTGYPDNSKFYKRGSVTLLR"
     gene            4497..7400
                     /gene="uvrA"
                     /locus_tag="PKY09_05570"
     CDS             4497..7400
                     /gene="uvrA"
                     /locus_tag="PKY09_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB12359.1"
                     /translation="MQEIESLNPKKYIIIKGASVHNLKKVDVAISRNSFTVITGLSGS
                     GKSSLAFDTLYAEGQRRYVESLSSYARQFMGRLDKPEVEYIKGIAPAIAIEQKVNTRN
                     PRSTVGTSTEIYDYLKLLFARIGKTYSPVSGRLVKKNTVADVVDFLKTQNQNKRFIIA
                     AQLQYHPGRSLHEELNALMQKGFSRIVVDDKMLRIDEYAEQIGKTDVPPAVKTRGRKK
                     KIEPVEPITNSNIFIVVSRILIPENINEEFESSTADSVQTAYNEGHGRCSLIDITDET
                     LHFTNFSNYFEEDNIRFEEPSTHLFSFNNPYGACKNCEGFGSVIGIDENLVIPNKTLS
                     IFDGAVACWRGEKMNEWQQEFVLKAYKFNFPVHRPYYELNETDIKLLWEGNKTIKGLN
                     DFFKFVESQSFKIQYRVLLSRYRGKTTCPECKGTRLRADANYVKVNNKSITDIVLMPV
                     NVSVEFFNNIVLDNYEQKVAGRLLVEIRNRLQYLNDVGLGYLTLNRLSKTLSGGESQR
                     INLATSLGSSLVGSMYILDEPSIGLHPRDTQRLIYVLKSLQKAGNTLIVVEHDEEIMR
                     NADELIDIGPFAGTHGGKIVFQGNFEQLEHSAESVTAKYLTGKEFIHVPSKRRNWTNS
                     IEITGASENNLAHIDAKFPLGILTAVTGVSGSGKSTLVKKVLYPALKKVLGGYDDESG
                     QFEKLTGDFTTLNGVEIIDQDPIGKSSRSNPVTYVKAYDEIRALMADTPFARQKMMKP
                     SLFSFNVDGGRCEMCQGEGVVTIEMQFMADIQLKCETCQGKRFKNEILAITYREKNIF
                     DILSMTVDDAISFFSKETDERYVLFTNKIIARLQPLSDVGLGYISLGQSSSTLSGGEA
                     QRIKLASYLGIGHATTKTLFIFDEPTTGLHFHDIRKLLDAFTALIRNGNSILVIEHNM
                     DVIKCADWIIDMGPGGGNEGGKIVFAGTPEALSKCNESFTGKFLFEKLVAQQV"
     gene            complement(7406..8287)
                     /locus_tag="PKY09_05575"
     CDS             complement(7406..8287)
                     /locus_tag="PKY09_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB12360.1"
                     /translation="MIINIVKSVASVVGLMVTRIPKTDYKDYLYKSLYSNDSLANRRF
                     YNVGAGPFKHKFWTNIDYINDWYADNTSNTLEGINYDLFSLEPLPIETDSAEMVYTSH
                     TIEHVNNAAVQNLFNESFRILKPGGRLRVVTQDIKLSYRAYKENDRHFFFWIDWFSKP
                     ENYKRVNLRQPLSQESIAQIFLEDFAAQASEIPLHGAKHRISDSELERLFEEKSFEEV
                     LDYCTSLCDIEVQKKYTGNHINWFTVEKLNSMLKVAGFKNIYRSAYGQSYSPVMRDLH
                     FFDETLPGVSLYVEAQK"
     gene            complement(8356..11064)
                     /locus_tag="PKY09_05580"
     CDS             complement(8356..11064)
                     /locus_tag="PKY09_05580"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12361.1"
                     /translation="MKKIFLILITIAAMSINTSSAQCPPLGSFSGMTGDLRACPGMSL
                     TYTLTPVTNAISYTWNLPPGATISGQNPYTTTATVVTVDYGPLFSAPGNLCVTAYNGC
                     VNSAPYCKALTPAGAPSGASLISGSNFACPGNTETYSVTNQVNRVFDWTIPNNATLLT
                     GQGTNSITVQYNAGFNGGNICVRVGNGCAWSGFRCMTVNAGVPGTPTAISGPTEICTG
                     TATYSTSPSNGVPVTSYTWTVAPGVTLVGQGNDTVQITFPLNYNYTDIYVKANNGCGS
                     SSNRSIRVRATPETPQPISGLAAGICNGTTSYTVTSSPTAISYNWTLTGPGTILNGQG
                     TNNIDIDYPNTFVSGKVCVTITNACATGSPRCLLTSKNIAISTQPQDFETCNSTQAMF
                     SVSALGMNLQYQWRRNGTPLSNGGKISGANSDTLYITGADSTDADQYDVIVSNNCGSS
                     VTSNAATLDIKQVPAKPGAISGVQATTCPGTSGIIYSVPLQMDATSYLWTHSDGVQIL
                     NGQGSDAVTLAFDSTNNSGYSVYVYAVNECGNSLDSARSWTRYKLSTPTITGLARVCE
                     NMLGVNYTAAAVVGASSYTWTVPAGATLVIGQTTNAIVVDYGPTYTGGDVTVTASNIC
                     FTTPVKKLATVIDMPSVPTSLSGQVYGTCKTQLAYTAGISNHATSYLWTLPPNASIVL
                     GNFTNNVTVDFQNAGTGTYSLCVAGKNSCRTGTARCIQVRAIPERPAVIVANPSVFCA
                     NQTGVAFSTAGGVGADSYTWALPSGSTITAGQNTSSILADIGTNNGTITVKGVNLCGN
                     SSTRAYSMTLNCREGFEESDMLPSVQVAPNPASNWVKLSFNSAVAEQYTVTIFDILGK
                     RVHSEGGISSLGLNEHSILLNGLVKGIYIVSLQSNSVSSRQKLEIR"
     gene            11367..13913
                     /locus_tag="PKY09_05585"
     CDS             11367..13913
                     /locus_tag="PKY09_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNB12362.1"
                     /translation="MAKRKNLQAEVNTIDSVESKPDIKRKTVKPKTEKFNFKWLANEQ
                     LHQIIGLFCILISIFLTIAFISYLFTWKVDFDKVKNGWWALLTDRDLKVENWLGKTGA
                     LSANSFFYNAFGLSSFLFVFMLFLSGFKLVFRHALLPFGRTIRIAIFSLLFISIACGY
                     IFKNTSLFLFTGGTFGFETSQWLQSTIGNAGTGLLLGFTLLSFSIITFKFKLKLPALN
                     KNNIQPEEKDVLITEVQSENNINTIADVIPNEEEEIRNTSPVNFEIEPDMDKAISDLM
                     KPEDQQNQKSDSNNLELELNIETPTIEKVEPVKESNIPLAIENLNSTEETTGDSEEND
                     SSDLPDQVTLGEYDPTLDLSSYQYPVLDLLENYQSKSKLTHEELKAELETNKNKIVET
                     LGHYNIGIEKIKATVGPTVTLFEIVPQAGVRISKIKNLEDDIALSLSALGIRIIAPIP
                     GKGTIGIEVPNQSPEVVPMKAVLGSEKFINSDMDLPIGLGKTISNEVYVTDLAKMPHL
                     LMAGATGQGKSVGLNAILVSLLYKKHPSQIKFVLVDPKKVELTLFKTIERHFLAKLPG
                     EEEAIITDTRKVVNTLNSLCVEMDQRYELLKDAQVRNIREYNTKFVARKLNPNKGHRF
                     LPFIVLVIDEFADLIMTAGKEVETPIARLAQLARAIGIHLIIATQRPSVNIITGTIKA
                     NFPARLAFRVSSKIDSRTILDCGGADQLIGKGDMLLSLGADVIRLQCAYVETFEVEKI
                     TDFIGSQRGYPDAFLLPEFVGDNESNTGEVDLADRDPLFEDAARMVVQHQQGSASLLQ
                     RRLKLGYNRAGRLIDQLEAAGIIGPFEGSKAREVKFKDPMELETLLSNLK"
     gene            14022..14660
                     /locus_tag="PKY09_05590"
     CDS             14022..14660
                     /locus_tag="PKY09_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNB12363.1"
                     /translation="MTKKIIIFLLLLTQISFAQKDNRSQEILKSVSAKYKTFKSVSAT
                     FRLQIDDKKSKTNQTQNGSILINGDKYNLSITGQQIISDGKTSWTYLKDANEVQVNDA
                     QNKVDGISPTNIFSIYEKGFDSRFAEEKTEGGKAYQYIDLKPLDKGKPYFKIQLKINK
                     SEKTIAGATVHNNNGSIITYTIVKFTPNPAVKDSDFSFDSKKYPGVEVVDLR"
     gene            14750..16036
                     /locus_tag="PKY09_05595"
     CDS             14750..16036
                     /locus_tag="PKY09_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB12364.1"
                     /translation="MIHHDTIAVGAGLAGLSFAYHFDKNLPVFEALDTVGGLVRTIEY
                     KNCKFDLAPHLLHLRNPYVRELVFEKLGLEVENHRRKAHIYYDKTIIPYPFELNLLNL
                     CEQTKKDCLEGLDEVKSYSKEEETEIRKGSYRDYALKCFGKGIANHYLLPYNRKIWDT
                     EPSDMTCEFMSHIITADKEQIRKNAFENNTDSFGYNVEFYYPVHKGIQDLADIFAKQL
                     TNIKFNTSAVFFNTRNKTIEFSDGSIVKYDKAVSTVPLQTIILNSDRDDLKSLAKELV
                     FTSVYTVNLVIRGTLPDTHWMYFPDKDLSFYRISFPKNYFKKSTPADEQIIAVEVGSR
                     NHNLSIEEIERKVVEEIKQMPIFKIDELVFVHSIKIPVAYCIYEPKRPAIINKLINEL
                     EEAGIYQSGRYGKWEYAGMQDAIMDGKVLSEKLKSL"
     gene            16033..16737
                     /locus_tag="PKY09_05600"
     CDS             16033..16737
                     /locus_tag="PKY09_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB12365.1"
                     /translation="MTENCSIVIPVFNEEEILEKRIHLLVEDLKKRFKDFEIVLTENG
                     SVDNTKSIIRTLAEKYDMVVAEIDDEKADYGQALINGINRARFEYITIFELDYLDMVF
                     LQKSNELLSTYDLVIGSKKLSGDLDRRPFKRKLFTWLYNTGLKVAFNYKLTETHGLKS
                     FKKSKLAPITNGCVTRHAVWPSEFCIRASRDKNLKVTEIGLSMPIEEIRTTRIKAIKR
                     LKKTIDDILLLRRSIN"
     gene            16749..17720
                     /locus_tag="PKY09_05605"
     CDS             16749..17720
                     /locus_tag="PKY09_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB12366.1"
                     /translation="MAENYHYHRYAPSDLDEILALFSLSFNGLKLSPEYYKWRVLDTP
                     VNKNLVDLFKNDNGKIISHYAVCPVKSFYNGETSLSELSLITMTHPEYKSLGLFPKLA
                     AKLYERSFNEENIQLVYGFPNTNSHYSFVKKLAWNDVYMIPTMKKAFTTTVQGDKSYT
                     EIFNADQRFDKLWEQHTQKTDRFYANVRNSTFINWRFFNRPDKKYRLFAFENNNTVQG
                     YFVIKEYVNASTKEIDVVDFLFTHDFDTTAFFNSIAQLATELDAKTANMWIPYNTPLY
                     NLAEKSGYLPKEHITYLGYKPFNPGKKLDELRIPTNWYLTMCDSDVY"
     gene            17754..18383
                     /locus_tag="PKY09_05610"
     CDS             17754..18383
                     /locus_tag="PKY09_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNB12367.1"
                     /translation="MKNVLVVGAHPDDLEWMAGGTVAKIVSDGGKVHLITLTNGTWKD
                     ASGNYYRDKDIAISEAKKAASVLGYTIEHLDEPCLDVNFRDALVVQVLNRIDKINADT
                     IICPWIDDLHHDHEMTARIAVSASRRVPRVLMGMCNWYVFRTPFNPNFFVDISDTYSK
                     KMAALECYESEMKRIGGIWRKYHDNITSNYGLMVNVERAEGFISYKYRL"
     gene            18508..19014
                     /locus_tag="PKY09_05615"
     CDS             18508..19014
                     /locus_tag="PKY09_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12368.1"
                     /translation="MLFAFNASAQRKVRKPIGYGASAIYNFQAEGIGADLRVRIPTPV
                     NNLYVVPEISYYPPFNRYHELYAGGALQYDILRIRSYNLYLLGAAYYNFWINADDYAP
                     EGKKKHNFAPEAGGGLVRNRGCWRPFIENRYDFKWKEDNLRIGIYYYPGQCRKSTRRE
                     KCPTVKGI"
     gene            19029..19940
                     /locus_tag="PKY09_05620"
     CDS             19029..19940
                     /locus_tag="PKY09_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12369.1"
                     /translation="MKSNKLFIYLTAIILVAVTACRKDRVPQDDYQNMDSFYSDHEEE
                     EQEYTIDTNGTCPLVCKKGTKICVNAEMFELNGGGQVTYPFQLKVIELYSIKDMILRR
                     IPSVASGNPLETSAEIRVRAFKNGNEIFLKPGKKYFLETATLPSINSAMQSYYGYSSG
                     NNANNWTNTISNFIPGFVDTLSSISPTSNSYVTTPATTGWFSPALLHQSSSTTSITCS
                     LTGTNTQNLQVYVSFKGFKGVMQIQNLSGTNFPVGENVTLVVFGKKQNNQYVLDLQNF
                     TIAANQQIIFNLQDVSENDILNTLATL"
     gene            complement(19953..20975)
                     /locus_tag="PKY09_05625"
     CDS             complement(19953..20975)
                     /locus_tag="PKY09_05625"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNB12370.1"
                     /translation="MIRKVFIPALILILLILGFYTRHWLVYGFNQLKGQLNIIYNAQP
                     ISEVIENPLTPDSVKAKLILIREIKKYAVDSLGLAETNNYETYYDQKGKPALWVLTAA
                     PEFKMEPYKWKFPFLGSLSYKGFFQKQKGVDELQLLKKKGFDADLSTVSAWSTLGWFG
                     DPVMSSMMRKNVGSLAELIIHEMTHTTLYVKGNVEFNENLATFIGEIGAEKFLTHKFK
                     DDSNVLTNYINHRKDNRIFSEYVVTSVAKLDSLYKDVDFISLTYPDKLRLKYQKIALI
                     VLHISKLPLKNHQRFEWNLQDKRLPDNTWFLSYSDYHALQPEIYQVYYDSCNSSIREL
                     INHYKK"
     gene            complement(20998..22791)
                     /locus_tag="PKY09_05630"
     CDS             complement(20998..22791)
                     /locus_tag="PKY09_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB12371.1"
                     /translation="MSAIQEKNQSVKGGEFIIKESNAENTFIPEEFSEEHLMIEQTAN
                     DFLKQEVHTVLDRIDAMEAGLMPSLLDKAGELGLLGVSVPEQYGGFGKDFITGTLLTE
                     TLGAGHSFSVAMAAHTGIGTLPIMYFGNTQQKEKYLPLLASGKWKASYCLTEPGSGSD
                     ALAAKTKAVLSADGKHYILNGQKMWITNAGFADVFVVFAQVDGDKFTGFIVEKTYPGL
                     SLGEEEHKMGIKGSSTRQVFFSDCKVPVENVLGEIGKGHLIAFNILNIGRLKLCAAAL
                     GGAKHVSTHAVSYANTREQFKLPIAKFGAIRYKLAQQAIRIYACESALYRTAFMIDEL
                     EKHKLADGIEYSQALLQAAEEYAVECAILKVDGSEALDYVADEGVQIYGGYGYSADFP
                     MDRAYRDSRINRIFEGTNEINRLLTVDMILKRAMKGKIDLMTPAMNIQKELMAIPDFS
                     EPDMSLFAQEKKFVANYKKALLAVAGSAVQKLMMTLAKEQEILMNIADMAIAIYISES
                     ILLRTEKLVKLKGEENCSVQLDMMRVYVNDAADRIALAGKEAINSFAEGDEQRMLLMG
                     LKRFTKVPAFNVKEARRRIAARLIEKNEYCF"
     gene            22937..28750
                     /locus_tag="PKY09_05635"
     CDS             22937..28750
                     /locus_tag="PKY09_05635"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB12372.1"
                     /translation="MHFSVKLAKINILVGGNIALNKVSGLWLTALVIITSLHQCLAQI
                     PVASFGISQSSGCTPLTVSFNNTSTNAASYHWDFGNGVVSVLHSPVITFTTPGDFFVK
                     LIATNASGSDSIISSQPIHIENKPVVQFSLNKSSACQGEVVNFTNQSAVYDSCQWDFG
                     DGVTSNQTNPTHIYATAGTYNVTLVLFRLNSSCTQTLTKNNIVVIKPVPVVTATVDTF
                     STCNINKIFLFHASANTTSTYQWNFGDGGTASGSNVQHQYSQPGNFQVAVTATASGCF
                     STVNLTSPVTVLNNPIPVITSNIASGCNPLTVVLSTNAIGTSSIQWSLGNGQTSTLST
                     VGVLYNNAGNYNPYLTATYPNGCSSQSAPLTIHALQSPVSTFTTSNTTGCKPLNVSFQ
                     TNACSGCSYQWNFGDGNSSSLFNGNNTYTQSGIFYPTLTVTAANGCTATSNLPYGITV
                     NGPEAGFLADKVTGCNPLTVNFANTSIGSTSWLWKFGTGDTSTANSPTYLYQNQGSFN
                     VTLIVFDGNGCKDSLRKQGYINPGPSVNNFSNPQPVSGCAPLTVSLSDSSGANSWLWN
                     FGDGSTGTAQNVSHTYNTPGTYTVSLQTQSNGSTCSQTISNFATYIVGGGIADFTYNQ
                     TLCPPFIGTFTDSSLNAVSWQWNFGDGQTSTQQNPVHTYNHTGSYNVSLTITSANGCT
                     STKVEQYAMNFLNLGAAPSAHTLDTIPPLDVQFSANSVGATSWLWTFGDGDSSTQENP
                     MHTYQSTGPFTISLTIANDSCSYTYSFPPTNFGSGGTFYDENHDTTQFTMHYDGCAPL
                     TISFNNPFLNVVGSHWDFGDGNTSSQQNPVHTYNVPGIYDVMLIANLSNASVDTIYHS
                     QAVKVAGTPVTFTVNSVNTCNGSNIQALPSNPNLQSYNWDFGDNQTSTQVSPVHSYAN
                     TSNYIISLQAQDTSGCTVIHSKSLYANADANAILSNKNRACASDTITFSVQNTNYVNY
                     TWDFGDGTSATGNPISHIYSDSGTYSVQVVITDINACTQTINAVQPINIVKPVADFSI
                     NMLSSSCTMVNVQLTNTSTGSTSYTWDLGDGTMSSSTDVNKYYYYGPNPPYTYDIKLT
                     AYNNGCLSTKTLAQAVTIPNFDVKFSYTRSQGCLPITINVTDSSLNAVSWKWSWGDGD
                     TTFTQASQHTFTTVPQDKIKLFAKDVNGCEKNYELDNIQLPAAVFMQSDTIGCNPKQI
                     QFTDSSKNAYQWLWKFGNGSTSTVQNPGVNYNSTGNFTPTLIITDSFGCKDTVDGKTI
                     AITEPQADFSYTKSAGCIPVIVQFQNASSGATNYLWNFGDGSTSTLQNPNHIYTTVGN
                     YQVTLTVTDASGCSNTKTLTNALHIPGPLASFSLSNNYGCQPLTISLNDSSQNALSHF
                     WSFGDGDTSVNASPTHTFTTAGSYLVTLIVKDANGCQSVFTHPDTIKVYNKPVAAFSV
                     SDSSICVGSSISLLNLSQSSSSYKWQLGDGSTSQHYNVNHPYQTPGQFSITLVASNGY
                     CSDTANSINAVTVIPFPIAEFGGSPLIGCIPLSSSMVNISTSLQNPVFMWDGGNGLTS
                     SAFNPQFNYQNAGYYSVTLKVTNEGLCSDSITKLNFIAAQNNQPITPSEISYITVLDS
                     NAIRISWQPSSATNLNRFELYRSTDNKNYNLIYTDSNSNNSTLNQQLAYIDNNVTTNQ
                     NRYYYKILTINNCNNSYPLDSSRAHASILLTTAPDTMSNTLSWLPYIGAPISQYSIYR
                     SIDGLNYDLIDAIAGTEDFYIDSTAYCPQEYSYKIEAQLDGSIYTSESNNDASVPTSL
                     VNQITTSVIRSTVIDNNSILTEWYEGTPFPELVYYYLIYKSSDKNPGNFILIDSVNNS
                     TKYYLDRDVDVNTFTYQYKIEVENICQQRTGLTDLSGNILLQGKLDDMNNSRLEWTPY
                     KGWKEGVERYEIQKQDENGNWKTIKSVDGNTHHMEEE"
     gene            28822..29337
                     /locus_tag="PKY09_05640"
     CDS             28822..29337
                     /locus_tag="PKY09_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HNB12373.1"
                     /translation="MQNKKILITGYSQCGKSSIVNRLVNNRFHHSYSEDNQLCIARKE
                     LAINKRKVNLVIWTAPGSVSNLKSFKSYYLGTDALIHVVDVNKPSTFSDLDAWISHYQ
                     KFLPGTPVYLTCNKIESNINLDCSDFFTQFDCYRTFFTSAKNNFMITEMFQTIAADLL
                     SEPFYQKIKIY"
     gene            complement(29745..>30872)
                     /locus_tag="PKY09_05645"
     CDS             complement(29745..>30872)
                     /locus_tag="PKY09_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNB12374.1"
                     /translation="KQPDAYLQCFEDLIFQKSNKGINTVAEFLDWWDVESNQNKISVQ
                     VPADENALTIITIHKSKGLQFPIVIIPYADWDLNSSKGLIWIRSDVSPFDKLGAFPVL
                     PSKKLLNTYFREAYILENRGKVLEEINTLYVACTRAEDKLFILISDSGKESEDGISKV
                     NTLIKSAIINADTVSSVLTKASDTVFTLGVDSKYIHKRKKAADVPDSFTHKELINYYA
                     RENRISTGFKIKKFAEQSKSRLIGIKVHELLADFRSEADLEKIENHLSNLQIPDEEKN
                     ELLNGVKATCKFLSEKKWVSPYYQIYNEHEMADAEGNILRPDRLMLEGDKAIVVDYKT
                     GAIEADHVHQIANYKEVLQDAGFKVVKTFLYYLEKKELIEA"
BASE COUNT        10123 a         5929 c         5283 g         9537 t
ORIGIN      
        1 taccaaaaaa attgcgcggc atatagttat ctgaatggag gctccgctaa gaacctgttg
       61 tcaaatgaac aatacccgcg ctaacgcgag cattagtttc atcttgacaa cttttgaaga
      121 tttagcggat ttccattcaa gaatttgcta acgcaatatt ttatgtatgc tttttttatg
      181 tcatttttta gtggtttaaa attgtggtta caagtatagg aataaatttt gaaatggaga
      241 aagatttatg gcttttctat aaataattat tgctataatt ctatttatac ttacagaaga
      301 aggagtaata tgtgaaaata tatcagcaac tatatatcta tttcaaatat aaaaaaatag
      361 atacaaccca ttatcaattc aaacaaatta aaaatgactt gaacaacaga acaggaaaaa
      421 aatcaacttc aaatctccta aagaggtact tttgtatgaa acttcaaatt agaattcagc
      481 aatatacaaa tcatctaatt tattgcatta atgtcactca aatttctcca tatgaaggag
      541 ttgaatacta ataaactact ttttttcaag caatgccaca cattctatat gatctgtctg
      601 tggaaacata tctaccggct ttactttcac caatgtgtaa tgatccttca tcagcgcaat
      661 atcacgagcc attgttgatg ggttgcaact aacataaaca attcttccgg caccgctatt
      721 acagattcta ttcactacat caggatgcat tcctgcgcga ggcggatcgg ttattatcac
      781 atccggaaaa ccatttgctt caaaaaaaga tgcatctaaa gtttcacgca tatcccctgc
      841 aaaaaatact gtattataaa ttgcattgct ttctgaattt aaccttgcct gcacaataga
      901 gtcttcaaca ttatcaatac ctattaccct cttacattgc ttagccaaaa aattcgcaat
      961 ggtacccgtt ccggtgtaca aatcataaac agactcttgt ggttgcaatg ctgcaaatga
     1021 caatgccgtt ttgtacaact catttgcctg aacaggattt gtttgaaaaa aagtttttgg
     1081 gcctatagta aattttaaac ctcccatact ctccgttatt gtctctgtgc cactccataa
     1141 aaccggctgc aaatcaaaaa atgtatcgtt cttcttttgg ttaataaagt aataaagtga
     1201 agttatttcg ggaaattctt ctttaattgc attaagcatt ctacttcgct tttcttcatc
     1261 ggcatctgca aacattataa ctaccatcca ctgtcctgat gtagtgtttc ttaccagcat
     1321 atttcgcaaa tcaccttgtt gactgatacg attgaaaaaa gtataatttt cttttaaagc
     1381 aaaacttttt acaaaattgc ggatacgatt tgtgggctcc tgcattaaca aacattcgtc
     1441 aatatttaaa accttatcaa agcgcccggt tatatgaaat cctaatgcag gttgaatctg
     1501 atattcctta ttctgaagtt tatctgctgt taaccattcc ttttcagaga aagcaaaatc
     1561 aagtctgttt ctatattgat aaacttgagc tgaaggcaat atttcttcca tcacaggatt
     1621 cgcaattcct gcaatccgaa ccaaagcatc tctcacttgc tttgttttat aaaacagttg
     1681 ctgctcgtac tgcatgtttt gacgtttaca tcctccacaa acaccaaaat gcttgcatga
     1741 cgggtttgta cgatggtccg aatatttcaa atattctgta acggcagctt caataaattt
     1801 attcttctta cgttttatat cagcatttat tatatcaccc ggcacaacat gatttacaaa
     1861 gactaccctc ccatccatgc gaccaagtcc gcgaccctcg tctgcaatat ccgtaatttc
     1921 aagattttca attctttttg tcattgcggc aaaaatatta ttattagaac aacttcttag
     1981 tgttcgtttt ttgtcatttc acccaaatta atatcctgac aatgacgtat tttactattt
     2041 ttgctataat taagaaattg cgttagatgc cattaaaaca tcgtttactg ttattcttgc
     2101 tgcttatgtt tggttttggt tacgaagtca atgcaaaaca tattgccggt ggcgatttta
     2161 cctacagacg acttaatggg aataactttg aaattacact taaaattttt cgtgactgct
     2221 ctgattttgt agcttttgac aatgctattt taattggtgt atttaacaaa ggaaccaata
     2281 cgttagtcaa ctcatatacc gttgcactta cagactccgg tacactgaca ctaaccggaa
     2341 cttcgtgcct gactccacca acaggtgttt gtatggataa atgtacttat gtagatacaa
     2401 tttcactgcc caacaaccca agtggatatt atgtgtcgtg ggaacgctgt tgccgtaata
     2461 atgccatcgt aaaccttact cgacccggtg ataccggcat tgcctattac atggaaattc
     2521 ctgaccctgc aattattgac aactcaccca actttaataa cgacccgttt ccttttatgt
     2581 gtctcggtca agattttgat ttcgattata gtgcctatga tgttgatggt gatttactga
     2641 cttatagtct tgaaacgcct ttagccggtg aactcggtta tccattaagt tcaactattt
     2701 cccctgtttg gtctaatccc actcccggac cttatcagca agtccaatgg cttcccggtt
     2761 attctgtagc taatattaca gggagctttc ctgccttgtc tttgaacagc agtacaggtc
     2821 ttatgaaagt aaaagcaaat aaagtaggct tttatgcaat ggctgttgta attaaagaat
     2881 tcagaaatgg tgttcagatt ggccagataa gacgtgaaat agaatttact gtaataaact
     2941 gtgcagccaa tacagcacca caaatcagct atcagaactc aactgctcct caaagtggat
     3001 taacgtttac aatttacgaa aaagacacgt tgtgttttac cgttcgtgtt actgattccg
     3061 attcaattaa gctttcatac agtggtgata tttttgctgg cggcagtatt aacccacctt
     3121 atgcggtaac atctgcggct tcaggcaatg gtgttgctac aagttctttt tgctggtaca
     3181 catcatgcga tcagggtcga acacaacctt acagtgttac atttaaagca acagataatg
     3241 gttgcccgca accattttca tctaccgact cattaaaaat atatgtactt ccggttcctc
     3301 aaattaccgc accacttatc atttgcatga acactgctac tgaaggaact ataaaactaa
     3361 ctttcacaga cacttttgca atgccaagat ttattgatag ctatgttgtt ttcagaagta
     3421 ccaatggtgg tccatatata aatatcggaa ctattaataa tcctgcaaat catttttaca
     3481 atgacactac tgctgcagat cttaacctga acaactactg ttattacatt gttgccgtaa
     3541 atacttgcaa tgaaacagga gcacattcag acaccatttg ctcagatact caaaaaaata
     3601 ttgatgcccc gtttattttt agttctcagg ttcaaaacaa caatagcatc gtgcttgact
     3661 ttaacaaccc gcctgataat ttattcactt tgtttaaaat tgaaaagcgc gaaaactcat
     3721 tttcggcaat atatgcagac tactttcaac aacaaggatt tgcaatacca caatggacag
     3781 actcgcttca aaatgtaact gaaaaatcat attgctaccg tatcacaaag aaaaatcaat
     3841 gtgatattga aagcccggta agtaatgagt catgctctat tttactcaca gggcaaagct
     3901 tgcctttgca acatattcta aactggacag cttatacaga atggcccggt ggtgtaagtt
     3961 attatgctat ttatcgcaga tcaaataact caaatacatt cacagaaatt gcacaattag
     4021 cttccaatat attaactttt acagacacac aaattccgca agataatggc atctttgttt
     4081 attacataaa agctgttcac gacactttaa atgttttttc taccagtaat gaagttacat
     4141 taattcagga accatcagtt tttacaccca actcatttac acccaatggc gatggtttga
     4201 atgaaacctg gtcaccggta tcttcattca taaaaaatta taatcttaaa attttcaatc
     4261 gttggggtaa tcttattttt gagtccaacg aggcaaatcg tcaatgggat ggcagatatc
     4321 agggtcaacc tgcacctctt ggcgtttatt tttacgttgt cacctatact gggtatcctg
     4381 acaacagcaa gttttacaag cgtggctcag tgaccttatt acgttaaagg agttttcaac
     4441 aataacatta caatggcatt ctgtgtgaca atagccatta attttgtagc atcattatgc
     4501 aggaaattga aagtttaaat cccaaaaaat atataatcat taaaggtgca tctgttcaca
     4561 acctgaaaaa ggtagatgtc gccataagca gaaacagttt tacagtaata accggattat
     4621 ccggttcggg taaatcatcc ttagctttcg atacattgta tgcagagggg caaagacgtt
     4681 atgtagaaag tttgtcaagt tatgcccgtc agtttatggg acgcttagac aaacccgaag
     4741 tagaatacat taaaggaatt gctccggcta ttgcaataga acagaaagta aataccagaa
     4801 atccaagatc aactgttggc acttcgacag aaatttacga ttatcttaaa ttactatttg
     4861 cacgaatagg aaaaacttat tcgcctgtaa gcggtaggtt agtcaaaaag aataccgttg
     4921 ctgatgttgt agatttctta aaaacacaaa atcaaaacaa gcgttttatc attgcagcac
     4981 aattgcaata tcatcccgga agatctttac atgaagaatt aaatgcattg atgcaaaaag
     5041 gatttagcag gattgttgtt gatgacaaaa tgttgcgaat agatgaatat gctgaacaaa
     5101 taggaaaaac agatgtacca cctgcagtta aaacaagagg aagaaaaaag aaaattgaac
     5161 ccgttgaacc aataactaat tcaaacatct ttatagttgt ttcaagaatt ttaatacctg
     5221 aaaatatcaa cgaagaattt gaatcatcta cagcagattc agttcagact gcatataatg
     5281 aaggccacgg tcgttgctct ttaattgata taacagatga aactttgcac tttactaact
     5341 tcagtaatta cttcgaagaa gataatatac gttttgaaga accttcaact catcttttca
     5401 gttttaataa tccttatggg gcatgtaaaa actgcgaggg ttttggaagt gtaattggta
     5461 ttgatgaaaa cttagttata ccgaacaaaa cactttcgat ttttgatgga gctgtggctt
     5521 gttggcgtgg tgaaaaaatg aatgaatggc aacaggaatt tgttttaaag gcatataaat
     5581 ttaatttccc tgtacatcgt ccctattatg aattgaatga aacggacatc aaattgttat
     5641 gggaaggcaa taaaactatt aaaggcctca atgatttttt taaatttgta gaatcgcaat
     5701 ctttcaaaat acaatatagg gttttgctta gccgctatcg cggaaaaaca acttgccctg
     5761 aatgtaaagg aactcgttta cgtgccgatg ctaattatgt aaaagtgaat aataaatcta
     5821 ttaccgatat tgtattaatg ccggtaaatg tcagtgttga attttttaat aacattgttt
     5881 tagacaacta tgaacaaaaa gttgcagggc gattattagt tgaaataaga aacagattgc
     5941 aatatttaaa tgatgtgggc ttggggtatc tgacattgaa cagactttca aaaacacttt
     6001 caggaggcga gtctcaacgt attaatctgg caacatcctt aggcagcagt ttggtaggaa
     6061 gcatgtatat attggatgag ccaagcatag ggttgcatcc acgtgataca caacgtttga
     6121 tttatgtttt aaaatcactt caaaaagcgg gaaatacact cattgtagtt gagcatgatg
     6181 aagagattat gcgtaatgct gatgagctaa ttgatatagg cccgtttgca ggtactcatg
     6241 gaggcaaaat tgtttttcag ggcaactttg aacaattaga gcatagtgca gaaagtgtga
     6301 cagccaaata tcttaccggc aaagaattta ttcatgtgcc aagtaaaagg cgaaactgga
     6361 ctaacagcat tgaaataacc ggtgcaagcg aaaataattt agcacacatt gatgctaaat
     6421 ttccacttgg tattctgacg gctgttacag gagtcagtgg gtcgggtaag tcaaccttgg
     6481 taaaaaaagt tctttatccc gcacttaaaa aagttttagg cggctatgat gatgagtcag
     6541 gacagttcga aaaactaact ggtgatttca caacattgaa tggggttgag attattgatc
     6601 aggaccctat aggtaaatca tcaaggagca accctgtaac ctatgtaaaa gcttatgatg
     6661 agattagagc attgatggcc gacacgcctt ttgccagaca aaaaatgatg aaaccttcat
     6721 tgttttcttt taatgttgat gggggaagat gtgagatgtg tcagggcgaa ggagttgtaa
     6781 ctattgagat gcagtttatg gcagacatac agttaaagtg cgaaacatgc caaggtaaac
     6841 gcttcaaaaa tgaaatactg gcaattactt accgtgaaaa aaatatcttc gatattcttt
     6901 caatgactgt tgatgatgcc attagctttt tttctaaaga aactgatgag cgctatgttc
     6961 tttttacgaa taaaattatt gccagactac aaccactttc agatgttggc ttaggctata
     7021 tttctttagg acaatcttcc agcacgctca gtggtggtga agcacaaaga ataaaattag
     7081 catcgtatct tggcataggt catgcaacaa caaaaacact ttttatcttt gatgaaccta
     7141 caacgggctt acattttcac gacatcagaa aactacttga tgctttcacg gcattaatac
     7201 gcaatggaaa ttcaatattg gttatcgaac acaatatgga cgtcataaaa tgtgctgact
     7261 ggattattga tatgggaccc ggtggcggca atgaaggtgg taaaattgtt tttgccggaa
     7321 ctccggaagc tttgagcaag tgcaatgaat cttttacagg aaaatttctt ttcgaaaaat
     7381 tagtagcgca acaagtttaa caactttact tctgtgcctc aacatagagt gaaactcctg
     7441 gcaatgtttc atcaaaaaaa tgaagatctc gcataacagg agagtaactt tgtccataag
     7501 ctgaacggta tatgttttta aatccggcta cttttaacat gctatttagc ttttccacag
     7561 taaaccagtt gatgtgatta cctgtgtatt ttttctgcac ttcaatatcg cataatgaag
     7621 tacaataatc aagcacttct tcaaaagact tctcttcaaa taatctttct aattcactgt
     7681 cagaaatacg atgtttggct ccatgtaaag gaatttctga tgcctgtgct gcaaaatctt
     7741 caagaaaaat ttgtgctatg ctttcctggc ttaaaggttg tctgaggttt actcttttat
     7801 aattttccgg cttactgaac caatctatcc aaaagaaaaa atgcctatcg ttttctttat
     7861 acgctctgta cgacaactta atatcctgcg ttacaacccg caatcttcct ccgggcttca
     7921 atattctgaa agattcgtta aacagattct gaacagctgc attatttaca tgctcaatgg
     7981 tatggctggt ataaaccatc tcagctgaat ctgtttcaat tggcaatggt tctaatgaaa
     8041 ataaatcata gttaataccc tctaatgtat tgcttgtatt atcggcatac caatcgttta
     8101 tataatcaat attcgtccaa aatttatgct taaacggacc tgcaccaacg ttgtaaaacc
     8161 ttctatttgc aaggctatca ttactatata aacttttata aaggtaatct ttatagtctg
     8221 tcttaggtat tcttgttacc atcaaaccaa ctacacttgc aacagatttt acaatattta
     8281 taatcatatg tatttattaa aattgcaaag tcacttaaat aagcgacttt gcaattacct
     8341 ccaataaata agagattatc ttatctcaag tttttgtctg ctcgagacag agttactctg
     8401 caatgaaacg atatatattc ctttaaccag tccattcaaa agtattgaat gttcattcag
     8461 tcctaaagaa gatataccac cttcgctatg tactctttta cctaaaatat caaaaatagt
     8521 tacagtatat tgttctgcta ccgctgaatt aaatgacaac ttaacccaat tgcttgcagg
     8581 atttggagca acttgtactg atggtagcat atctgattct tcaaagcctt cacgacagtt
     8641 aagtgtcatt gaataggcac gtgtactact gttgccacat aaattaacac cctttactgt
     8701 tatggttcca ttattggtac caatatcagc taaaatacta cttgtatttt gaccggctgt
     8761 gattgttgaa ccactgggta gtgcccatgt gtaagaatca gcaccaacac ctccggcagt
     8821 agagaaagca acaccagttt ggttagcaca gaaaacagaa ggatttgcaa caattacagc
     8881 tggtctttct gggatagcac gtacctgaat acaacgtgcg gtgccggttc tacaagaatt
     8941 ttttcctgca acacaaaggc tatatgtacc ggttccggca ttctggaagt caacagttac
     9001 attattggtg aaatttccaa gaactattga tgcgtttggt ggtaatgtcc ataaataact
     9061 agttgcatgg ttagaaattc ctgctgtata tgctaactgt gttttacagg taccataaac
     9121 ttgtccactt aaagaagtag gaacagaagg catatcaata accgttgcaa gtttttttac
     9181 tggggtggtg aagcaaatat ttgaagctgt tacagtaaca tctcctcctg tgtatgtagg
     9241 cccgtaatca actactattg catttgttgt ttgaccgata actaaagttg ctccggcagg
     9301 aacagtccaa gtataagaag aagctccaac tactgcagct gctgtataat ttacaccaag
     9361 catgttctcg caaactcttg caagacctgt aatagtaggt gttgataatt tatatcgtgt
     9421 ccatgatctg gctgagtcta aactattacc gcactcattt actgcataaa cataaactga
     9481 atatcctgaa ttatttgttg aatcaaaagc taaagtaaca gcatcactac cctgaccatt
     9541 taaaatttga acgccatcag aatgcgtcca taaataactt gttgcatcca tttgtaaagg
     9601 aactgaataa attatacctg aagttccagg gcaggtagtt gcctgtacgc cactaattgc
     9661 tcccggttta gccggcactt gtttaatatc aagtgtggct gcattcgaag taactgatga
     9721 cccacaattg tttgatacaa taacatcata ctgatcagca tctgtactat ctgcaccggt
     9781 aatatataat gtatcactat ttgcacctga aattttacct ccattactca aaggtgtacc
     9841 atttctgcgc cactgatatt ggagattcat tcctaaagca cttacagaaa acatggcctg
     9901 tgtactgtta caggtttcga aatcctgagg ctgagtactg atagcaatat tctttgaggt
     9961 aagcaaacaa cgtgggctgc cggtagcaca tgcatttgta atggtaacac aaactttacc
    10021 tgaaacaaat gtatttgggt aatcaatatc aatattattt gttccttgtc cgtttagaat
    10081 tgttccaggg cctgttaaag tccagttgta tgaaattgca gttgggcttg aagtaacagt
    10141 ataacttgtt gttccattac aaattcctgc tgcaagacca ctaataggtt gtggagtttc
    10201 cggagttgct cttacacgaa tacttctgtt agagcttgaa ccacagccat tatttgcttt
    10261 tacataaata tcggtataat tatagtttaa tgggaaagtt atctgaacag tatcattacc
    10321 ctgtcccaca agtgttactc caggagccac agtccaggta taagatgtaa ccggtacgcc
    10381 attagatgga ctagtactat aagttgctgt tcctgtacaa atttctgtag gaccggaaat
    10441 tgctgttggt gtacccggaa cacctgcatt cactgtcata catcgaaacc cactccatgc
    10501 acatccgttg ccgacacgca cacaaatatt tccaccatta aaacctgcat tgtattgaac
    10561 agtaatactg ttggtgcctt gccctgttag cagtgttgca ttattgggaa tggtccaatc
    10621 aaatactctg ttcacctgat tagttactga ataagtctca gtatttccgg gacatgcaaa
    10681 attagatcct gaaataagtg atgctcctga tggtgcacct gcaggtgtta atgctttaca
    10741 atatggtgct gaattaacgc aaccgttata agcagttaca cagaggtttc ccggagcaga
    10801 aaataatgga ccgtaatcta ctgttacaac agttgctgtt gtagtatatg ggttctgacc
    10861 actgatggtt gcaccgggag gcaaattcca agtgtaacta attgcatttg taacaggtgt
    10921 taacgtataa gttaatgaca tgccagggca tgcgcgaaga tcgccagtca ttcctgaaaa
    10981 cgatcctaaa ggagggcatt gtgctgatga agtattaata ctcattgcag caattgttat
    11041 taaaatcaga aagatttttt tcattttaat aagtgttgat tttgactttt tcattttgta
    11101 tgcggatatt tgatttaaat agaattaact tacttataca ctaaattggt taaagtttta
    11161 gggatgcatt ttatatacaa tttctctaat gcttttagta ttacaaaaat gcaatacaaa
    11221 acggggtgat ttaatgaaat ttacaaacac tgcaaccctt ttatccacaa taaaatgttt
    11281 attacatggt tttcttatag gaatagttga tttacattaa gcctaattta gctcattcaa
    11341 attacttctt tcacataaaa aataaaatgg ccaaacgcaa aaacttacag gctgaagtaa
    11401 ataccataga ctcggttgaa agcaaaccgg atattaagcg aaaaactgta aaaccaaaaa
    11461 cagaaaaatt taactttaaa tggttagcaa atgaacaatt acaccagatt atcggactgt
    11521 tttgtattct gattagtatt tttctgacca ttgctttcat ctcctatctt tttacctgga
    11581 aagttgattt tgacaaagtt aaaaatggat ggtgggcatt acttacagac cgggatttaa
    11641 aggttgaaaa ctggttaggc aaaacaggag cattatctgc aaattcattt ttttacaatg
    11701 cttttgggct aagcagtttc ttatttgttt ttatgttgtt cttatcagga tttaaattgg
    11761 ttttccgaca tgccctactt ccttttggac gtactataag aattgcgatt ttttcactgc
    11821 tctttatttc aattgcatgt ggatatattt ttaaaaacac cagtttgttt ttatttacag
    11881 gtggcacatt cggatttgaa acatcacaat ggcttcaatc tacaatagga aatgccggaa
    11941 caggtctatt acttggcttt acccttcttt ctttttcaat cataacattt aagtttaaat
    12001 taaaacttcc cgcactaaat aaaaataata ttcaacctga ggagaaagat gtattaataa
    12061 ctgaagtaca atcagaaaat aatataaata caattgcaga tgtaattcct aatgaagaag
    12121 aagaaataag aaacacttct cccgtaaatt ttgaaataga accggatatg gataaagcca
    12181 tttctgattt gatgaaacct gaagatcaac aaaatcaaaa atctgattct aataaccttg
    12241 aattggaatt aaatattgaa acacctacta tagaaaaagt tgaacctgta aaagaaagta
    12301 acattccact tgcaatagaa aatctgaata gcacagaaga aactaccggt gattcagaag
    12361 aaaacgatag cagcgatctt cccgatcagg ttacacttgg cgagtatgac cctacacttg
    12421 atttatcttc ttatcaatat cctgtgttgg atttactgga aaattatcag agcaaatcta
    12481 aacttacgca cgaagagtta aaggcagagt tagaaactaa taaaaacaaa attgttgaaa
    12541 cactcggcca ttataatatc ggtattgaaa aaattaaagc aactgttggg cctacggtga
    12601 cactgtttga aattgtaccg caagccggtg ttcgtatttc aaaaatcaaa aatcttgaag
    12661 atgatattgc tttaagtctt tcagctttag gtattcgtat cattgctcct attccaggca
    12721 aaggaacaat tggaattgaa gttcctaatc aatcacctga agtggtgccg atgaaagctg
    12781 ttttaggttc agagaaattt attaactccg atatggacct acccatcgga cttggaaaga
    12841 ccatttccaa tgaggtgtat gtaaccgatt tagctaagat gccacacttg ctcatggcag
    12901 gtgccaccgg tcagggtaaa tctgttggcc taaatgcaat actggtttca ttactttaca
    12961 aaaaacatcc atcgcaaata aaattcgtat tggttgatcc taaaaaagta gaacttactt
    13021 tatttaaaac tattgaacga cattttttag ccaaactgcc cggtgaagaa gaggcaatca
    13081 ttaccgatac acgcaaagtg gtgaacacgc ttaactcttt atgtgttgaa atggatcagc
    13141 gctacgagtt acttaaagat gctcaggttc gtaatataag agaatacaat acaaaatttg
    13201 ttgcaagaaa actcaacccc aataaaggtc accgctttct accttttatt gttttagtta
    13261 ttgatgagtt tgccgactta attatgacag caggaaaaga ggttgaaaca cccattgccc
    13321 gactagcaca gttagcccgt gccattggta ttcatctcat catagcaact caacgtccat
    13381 cagtaaatat catcacagga acaataaaag ccaacttccc ggcacgtctg gcattcagag
    13441 tttcatcaaa aattgatagt cgcacaattt tagattgtgg cggtgccgac caactcattg
    13501 gcaagggaga catgctattg tcactgggtg ctgatgttat tcgtttgcag tgtgcctatg
    13561 ttgaaacttt tgaagttgaa aaaattacag attttattgg ctcacaaaga ggataccctg
    13621 atgcttttct gttaccggaa tttgttggtg acaacgaaag caacacaggc gaagttgatt
    13681 tagctgaccg tgatccattg tttgaagatg ctgcacgtat ggttgtgcaa catcagcagg
    13741 gttctgcatc tttattgcaa agaagactta aacttggcta taaccgtgcc ggcagactga
    13801 tagatcaact tgaagcagca ggaattattg gcccctttga aggcagcaaa gccagagaag
    13861 taaagtttaa agatccaatg gaacttgaaa cgttgctttc taatctgaaa taattctgtt
    13921 tttaatcctc ctacattatt atatttgcaa ttgttcatgt gcatttcact tcgtttagcc
    13981 ttgtggcatt gtttttatta atcacaatta aaatttaaga aatgacaaaa aaaataatca
    14041 tcttcctttt gctgttgacc caaatttctt ttgctcaaaa agacaaccgc tctcaggaaa
    14101 ttcttaaatc agtatctgct aaatataaaa ctttcaagtc tgtgtctgca acattcaggc
    14161 tgcaaattga cgacaaaaaa tccaaaacca atcagacaca aaatggttcg attctgatta
    14221 atggtgacaa atacaatctg agtattacag gacaacagat tatttctgac ggcaagacat
    14281 catggaccta tttaaaagat gccaacgaag tacaggttaa cgatgcacaa aataaagttg
    14341 atggcatatc cccaaccaat attttctcaa tttatgagaa aggatttgat tcacgatttg
    14401 cagaagagaa aaccgaaggt ggtaaagcct atcagtatat tgacctgaaa cctcttgata
    14461 aaggaaaacc ttattttaag atacagttaa aaatcaacaa atccgaaaaa acaattgcag
    14521 gagccaccgt tcataacaac aatggctcta tcataacata cactattgta aaattcacac
    14581 caaaccctgc tgttaaggac agcgatttta gttttgattc aaaaaaatat ccgggtgtag
    14641 aggtggttga cctcagataa agtgtcctct tttaaggtaa ttgtagggct ttgaatgaag
    14701 gataaaaatc cttaatttcg caacctttta aatctcctta cttttctata tgattcatca
    14761 cgacacaatt gcagtgggtg ccggtttggc aggattaagt tttgcctatc attttgacaa
    14821 gaatttaccg gtttttgagg cccttgacac agttggtggt ttggtgcgaa ctattgaata
    14881 taaaaactgc aaatttgatt tagctccgca cctattgcat ttaaggaatc cttacgttag
    14941 agaacttgta tttgagaaac ttggtcttga agttgaaaat cacagaagaa aagcacacat
    15001 ctactacgac aaaaccatca tcccctaccc ttttgaactc aatcttttaa atctgtgtga
    15061 gcaaaccaaa aaagattgtc ttgaaggact tgacgaagta aaatcataca gtaaggaaga
    15121 agaaacagaa atcagaaaag gttcttacag agactatgca ttgaaatgct tcggaaaagg
    15181 tattgccaat cattacttac tgccctacaa cagaaaaatt tgggatactg aaccttctga
    15241 catgacatgt gagtttatgt cgcatattat tactgctgac aaagagcaaa tcagaaaaaa
    15301 tgcattcgaa aacaacacag atagttttgg atataatgtg gagttttatt atcctgttca
    15361 taaaggcatt caggatttag ccgacatttt tgcaaaacaa ctcactaata ttaagttcaa
    15421 tacttctgca gtatttttta atacaaggaa taaaactatt gaattttctg atggaagcat
    15481 tgtaaagtat gacaaagctg tttcaactgt tccgctacaa accatcatac taaacagcga
    15541 cagagatgat ttgaaatcac tggcaaaaga attggttttc acctctgttt atacagtaaa
    15601 tttagttatc cgaggaaccc tgcctgacac acattggatg tatttccctg ataaagatct
    15661 ttcattttac agaataagct tcccaaaaaa ctattttaaa aaatcaacac ctgctgacga
    15721 acaaattatt gctgttgaag taggaagccg caatcataat ctgagtattg aagaaataga
    15781 acgtaaagtt gttgaagaga ttaaacaaat gccgattttt aaaattgatg aactggtatt
    15841 tgttcactct attaaaattc cggtagccta ttgcatatac gaacctaagc gccccgcaat
    15901 tatcaacaaa ctgataaatg agctggaaga agcaggaatt tatcagtcag gtcgctatgg
    15961 aaagtgggaa tatgcaggca tgcaggatgc aattatggat ggcaaagtgt tgtcggaaaa
    16021 attaaaatca ttatgacaga aaattgttca atagtaatac ccgtttttaa cgaagaagaa
    16081 attcttgaaa aaagaataca cctgctcgtt gaagatttaa agaagcgttt taaagatttt
    16141 gaaatcgtat taactgaaaa tggaagcgtt gacaacacca aatctattat cagaacactt
    16201 gcagaaaagt atgatatggt tgttgcagaa attgatgatg aaaaagccga ttatggccag
    16261 gctttgatta atggaataaa tcgtgcacgt tttgagtata tcaccatatt tgaacttgat
    16321 tatttagata tggtcttttt acaaaaatcc aatgagttgc ttagtacata cgatcttgta
    16381 atcggatcaa aaaaactttc aggtgatctt gacagaagac catttaaaag aaaacttttt
    16441 acatggctct acaatacagg acttaaagtt gcattcaact ataaattaac tgaaacgcat
    16501 ggactgaaaa gttttaagaa aagtaaatta gcacctatca caaacggttg tgtaacgcgg
    16561 catgctgttt ggcctagtga attttgcata agagcttcac gtgacaagaa tttaaaagta
    16621 actgagattg gattatctat gccgattgaa gaaattagaa ccacacgtat taaagcaata
    16681 aaaagattaa agaaaaccat tgacgatatt ttactgctac gcagatctat taactaatca
    16741 catacgctat ggcagaaaat tatcattatc atcgctacgc tccatcagat cttgatgaaa
    16801 tacttgcttt attttctctt tcatttaacg ggctaaaact ctctccggaa tattacaaat
    16861 ggcgggtttt agacacacct gtaaataaaa acttagtaga tttatttaag aatgacaatg
    16921 gtaaaatcat aagccattat gctgtgtgtc cggtaaaatc tttttacaat ggcgaaactt
    16981 cacttagcga gctttcgctc atcaccatga cacatcctga atataaatca ttagggctgt
    17041 ttccaaaact tgcagcaaaa ctttacgagc gttctttcaa cgaagaaaat attcaattgg
    17101 tttatgggtt tccaaatacc aactcgcatt acagctttgt aaaaaaatta gcctggaatg
    17161 atgtgtatat gattccaaca atgaaaaaag cttttacaac tactgtgcag ggagataaaa
    17221 gttatactga gatttttaat gccgaccaac gatttgacaa gctttgggaa caacacacac
    17281 agaagacaga tcgtttttat gccaatgtca gaaacagcac atttattaac tggcgctttt
    17341 ttaatcgccc tgataaaaag tatcggttat ttgcatttga aaacaataat actgttcaag
    17401 gctattttgt aattaaggaa tatgttaatg catcaacaaa agaaattgat gttgttgact
    17461 ttctttttac tcacgatttt gatacaactg cattctttaa ttctattgca caattagcaa
    17521 cagaattaga tgctaaaaca gcaaacatgt ggattcccta caatacgcca ctgtataatc
    17581 ttgctgaaaa atcaggctat ctgcccaaag aacatataac ttacttaggt tacaaaccat
    17641 tcaaccccgg aaaaaaatta gatgagttaa ggataccaac aaactggtat ttgacaatgt
    17701 gtgattctga tgtttactga tagtttactt ttgaatttaa aatcaataac aacatgaaaa
    17761 atgttttagt ggtaggcgca catcctgacg atttggaatg gatggccggt ggcactgttg
    17821 ccaaaatcgt ttcagatgga ggtaaagtcc atttgataac tttaaccaat gggacatgga
    17881 aagatgcctc cggaaattac tatcgtgata aagatattgc tatcagcgaa gcaaaaaaag
    17941 ccgcttcagt tttaggctac acaatagaac atttagacga accttgtctt gatgtaaatt
    18001 tcagagatgc attagtagtt caggtactaa accgtattga caaaataaat gcagacacca
    18061 ttatatgtcc atggattgac gatttgcatc acgatcatga aatgactgca cgtattgctg
    18121 tcagcgcaag tagacgcgtg ccacgtgtac tcatgggcat gtgcaactgg tatgtgtttc
    18181 gtaccccttt taatcctaat tttttcgttg acatcagcga cacctattct aaaaaaatgg
    18241 cagcattaga atgctacgag tcagagatga aacggattgg cggtatatgg agaaaatatc
    18301 acgacaacat cacatcaaat tatggtttaa tggtgaatgt agagcgtgca gaaggcttta
    18361 ttagctataa atacagactg taattctgtt tataatcaaa taataatttt tcagtaatgc
    18421 cgttttggga atagattaag ttaacatctt taatcccaaa atatttccat gttaaaggtc
    18481 aggcatatat ccattatttt gtttgcaatg ctctttgcgt ttaatgcttc agcacaacgt
    18541 aaagtacgga aacctatagg ctacggtgcc agcgcaatct ataattttca agctgaaggc
    18601 attggagcag atttgcgtgt cagaattcca acaccggtaa ataatcttta tgttgttccc
    18661 gaaatttcat attatcctcc ttttaatcgc tatcatgagt tgtatgccgg aggagctttg
    18721 caatatgata ttttaagaat aagaagttat aatctttatt tacttggtgc tgcctattac
    18781 aatttctgga ttaacgcaga tgactatgcc cctgaaggaa aaaagaaaca caattttgct
    18841 cctgaagctg gtggtggctt ggtacgaaac agagggtgct ggcgtccatt tattgagaat
    18901 cgctatgact ttaaatggaa agaagacaat ttgcgaatag gcatatatta ttatcccgga
    18961 cagtgcagaa aaagtacaag aagagaaaag tgcccaaccg ttaaaggaat ttaatcaaac
    19021 tttgtaatat gaaatcgaac aagctattca tttaccttac tgcaataata ttagttgcag
    19081 taactgcatg cagaaaagat cgtgtgccac aagatgatta tcaaaacatg gattcattct
    19141 atagcgacca cgaagaagaa gaacaggaat atacaattga caccaatggc acttgtcctt
    19201 tagtttgcaa aaaaggaact aaaatttgtg tcaatgcaga aatgtttgag ttaaatggtg
    19261 gtggtcaggt cacttatcca tttcagctaa aagtaatcga gctgtattcg ataaaagata
    19321 tgattctaag aagaattccc tcggtggctt caggcaaccc acttgaaaca agtgcagaaa
    19381 taagagtaag agcatttaaa aacggaaatg aaatctttct taagcccggt aaaaaatatt
    19441 ttcttgaaac agctacattg ccatctataa attcagccat gcagtcatat tatggttata
    19501 gcagcggcaa caatgctaac aactggacca atactatttc taattttatt cctggttttg
    19561 ttgatacact atcaagtatt tctcctactt ctaacagcta tgttactaca cctgcaacaa
    19621 caggttggtt ttcgcctgca ttgttacatc agtcatcatc cactacatca attacctgta
    19681 gcctcacagg aacaaataca caaaacttac aggtctatgt ttcattcaag ggttttaaag
    19741 gtgttatgca aatacaaaac ctaagtggta caaactttcc tgttggcgaa aacgtaacgt
    19801 tggtagtatt tggtaaaaaa caaaacaatc aatacgtttt agatctgcag aattttacta
    19861 ttgctgccaa tcagcaaatt atctttaatc ttcaggatgt ttctgaaaat gatattctga
    19921 atacacttgc aacattataa gtaggttact tcctatttct tatagtgatt tatcaactca
    19981 cgaattgaac tattgcagga atcataatac acctgataaa tttcaggctg caaagcatga
    20041 taatcggaat aagaaagaaa ccaggtgttg tcaggcagtc ttttatcctg cagattccat
    20101 tcaaaccttt gatgattttt cagtggtaac ttcgatatat gtaaaacaat taatgcgatt
    20161 ttttggtatt taagacgtaa tttatccgga tatgtcaacg aaataaaatc tacatcttta
    20221 tacaatgagt ctaatttcgc aactgaagta actacatatt ctgaaaatat tctgttgtct
    20281 ttacgatgat tgatataatt tgtcagaaca ttgctgtcat ctttaaattt atgagtcaga
    20341 aatttttcag caccaatctc tccaataaaa gttgcaaggt tttcattgaa ctcaacattg
    20401 cctttgacat aaagtgttgt atgagtcatt tcatggataa tcaattctgc aagtgaccca
    20461 acattttttc tcatcattga cgacattaca ggatcaccaa accatccaag agtactccat
    20521 gctgacactg ttgacaaatc tgcatcaaag ccctttttct tcaacaattg taattcatca
    20581 acccccttct gtttttggaa aaaaccttta tacgataatg atccaagaaa tggaaacttc
    20641 catttatatg gctccatttt aaattcagga gcagctgtga gaacccataa tgcaggcttc
    20701 cctttttgat catagtaagt ttcgtagttg ttggtttctg ccaatcctaa agaatcaaca
    20761 gcatattttt ttatctcacg gattaaaatc agttttgcct ttactgaatc gggagttaat
    20821 ggattttcta ttacttcgct aataggctgt gcgttgtaaa taatatttaa ctgtcccttt
    20881 aattgattaa acccataaac cagccaatga cgcgtataaa atcctaaaat caataaaatt
    20941 aatatgagtg ccggaatgaa aaccttcctg atcatctgct tagaattgca ctaccaatca
    21001 gaagcagtat tcgttctttt caattaagcg ggctgcaatt cttcttcttg cttctttaac
    21061 attaaatgca ggaaccttag tgaaacgctt taatcccata agtaacattc tttgctcatc
    21121 tccttctgca aaggaattga ttgcttcttt ccctgctaag gcaattctgt ctgctgcatc
    21181 gtttacataa acccgcatca tgtcaagctg aacactgcaa ttttcttcac cttttaattt
    21241 tacaagtttc tccgtccgca ataaaattga ctctgaaata taaattgcaa tagccatatc
    21301 tgcaatgttc atcaggattt cctgttcctt tgcaagtgtc atcattaatt tttgcacagc
    21361 tgaacctgca actgccagta atgctttttt atagttagcc acaaactttt tctcctgagc
    21421 aaacaatgac atatcaggct cactaaaatc aggtatagcc ataagctcct tctgaatatt
    21481 catagccggt gtcattaaat caatcttacc tttcatggca cgcttaagaa tcatatcaac
    21541 agtaagtagt ctgttaatct catttgttcc ttcaaaaatt ctgttgatgc gtgaatctct
    21601 atatgcccta tccataggaa aatctgcact atagccgtag ccgccataaa tctgtacacc
    21661 ttcatcagca acataatcga gtgcctccga accatcaact ttaagaatgg cacattcaac
    21721 agcatattct tcggcagcct gaagtaatgc ttgagagtac tcaattccgt cagcaagttt
    21781 atgcttttcg agttcgtcaa tcataaaagc tgtacgatac aaagcgctct cacaagcata
    21841 aattcttatt gcctgctgtg caagcttata tctgatggcg ccaaattttg caatcggcaa
    21901 tttaaactgc tcacgtgtgt ttgcatagga aacagcatgt gtacttacat gctttgcgcc
    21961 acctaatgca gctgcacata acttcaatcg tccgatattt aaaatattga aagcaataag
    22021 atgtcctttg ccgatttcgc ccaagacatt ttctaccggc actttacaat cactgaaaaa
    22081 aacctgtctt gtacttgagc cttttattcc catcttatgt tcttcttcac caagcgaaag
    22141 tccgggatag gttttctcta caataaatcc tgtgaattta tctccgtcaa cctgtgcaaa
    22201 gactacaaac acgtctgcaa agcctgcatt ggtaatccac attttttgtc cgttaaggat
    22261 gtaatgcttt ccatcagcag aaagaacagc ttttgttttg gctgcaagtg catcagaacc
    22321 ggaaccgggc tcagttaaac agtaagatgc tttccatttg cctgatgcca ataacggcag
    22381 atacttttct ttttgttgtg tattaccaaa atacataata ggcaatgtcc caatgcctgt
    22441 atgtgctgcc attgcaacag aaaatgaatg acctgcgcct aaagtctctg tcagcaatgt
    22501 acctgtaata aaatcttttc caaatccgcc atactgttca ggaaccgata caccaagaag
    22561 tccaagttct cctgccttat ctaataatga tggcataagt cctgcttcca ttgcatcaat
    22621 acgatcaagc acagtatgca cttcttgttt taaaaaatcg ttggctgttt gctcaatcat
    22681 caaatgctct tcgctaaact cttccggtat aaaagtgttc tctgcatttg attctttaat
    22741 aataaattct ccgcctttaa ccgattggtt cttttcctga attgctgaca taataaaagt
    22801 ggttttttaa cgcaataaaa ataaaaatat taaacataaa taacagcctc agggtttcaa
    22861 tatttctaaa ataacgaaaa ttaaatctat tgaaaaacaa agaagtaaaa ttaaaatgta
    22921 agaaatataa cttttaatgc acttttccgt aaaacttgcc aaaatcaaca ttttagtggg
    22981 aggaaatata gctttaaata aagtgtcagg attgtggctt acggctctgg tgattattac
    23041 ttcactacac caatgtcttg cacaaatacc tgtagcatca tttggaatta gtcagtcatc
    23101 aggttgtaca cctctaactg tttcatttaa caatacaagt actaatgcag caagctatca
    23161 ttgggatttt ggcaatggcg tggtttctgt tttgcatagt cctgtaatta cttttaccac
    23221 tccgggcgat ttctttgtta aacttattgc aacaaatgca tcaggcagtg actctataat
    23281 ttcttcgcaa cctatacata tagaaaacaa acctgttgta cagttttcac taaacaaatc
    23341 ttcagcatgt cagggtgaag ttgtaaactt taccaatcaa agtgcggttt atgacagttg
    23401 tcaatgggat tttggtgatg gtgttactag taatcaaacg aatccaacac atatttatgc
    23461 aacagccgga acctataatg ttactttagt tctgttccga ctcaattcaa gctgcacgca
    23521 aacattgacg aaaaacaata tagttgttat aaagcctgtt cctgttgtaa cagctacagt
    23581 tgacacattc agcacctgca acatcaacaa gatatttctc tttcatgcat ccgctaatac
    23641 aacatcaact taccaatgga attttggtga tggaggaaca gcatcaggct caaatgtgca
    23701 acatcaatat tcacaaccag ggaactttca ggtagctgtc actgcaactg cttctggttg
    23761 cttttctact gtgaatctaa catcaccagt aacagtactt aacaatccta ttccagtaat
    23821 tacttcaaat atcgcctccg gttgcaatcc gctgacagtt gttctttcaa ctaatgctat
    23881 cgggacatct tccattcaat ggagtttagg caatggtcag acttctacac tatcaacagt
    23941 tggcgtctta tataataatg caggaaacta caatccatat ttaacagcaa cctatccaaa
    24001 cggctgttca agtcagtctg caccattaac tatacatgca ttacaatcac cggtttcaac
    24061 attcacaaca tcaaatacca caggatgtaa gccattgaat gtttcatttc agactaatgc
    24121 ttgttccggt tgcagttatc aatggaattt tggtgatggc aatagctcat ccctattcaa
    24181 tggtaataat acttacactc aatcagggat attctatcct acattaaccg ttacagctgc
    24241 aaacggatgt actgctacat caaatcttcc atacggaatt actgttaacg gacctgaggc
    24301 cggctttctt gcagataaag taacaggatg taatccatta acggtaaact ttgcaaatac
    24361 cagtataggc tcaacatcgt ggttatggaa atttggtaca ggtgacactt caacagcaaa
    24421 cagtccaacc tatctctatc agaatcaagg cagctttaat gttacactaa ttgtctttga
    24481 tggcaatggc tgtaaagact cattacgcaa acaaggttac attaacccag gaccaagcgt
    24541 aaacaacttc agtaatccgc aacccgtttc aggatgtgca ccacttacgg tttcattaag
    24601 cgactcatca ggtgcaaatt catggctttg gaattttggt gatggcagta ccggtacagc
    24661 acaaaatgtc tctcacactt ataacactcc ggggacatat acagtaagtc ttcagacaca
    24721 aagcaatggc tcaacatgct ctcaaacaat cagcaatttt gccacctaca ttgtcggtgg
    24781 tggtattgct gattttacat acaaccaaac actttgtccg ccttttattg gaacatttac
    24841 agacagttcc ttaaatgcag tttcatggca atggaatttt ggtgatggac aaacatcaac
    24901 acaacaaaat cctgttcaca cctataatca caccggaagt tataatgttt ctttaaccat
    24961 aacttctgct aatggttgta catcaacaaa agttgaacaa tatgcaatga actttttaaa
    25021 tttaggtgct gcgccatctg cccatacttt agacaccatt cctcctcttg atgttcagtt
    25081 ttcggccaac tcagtaggtg ctacatcatg gctatggact tttggtgatg gcgattcttc
    25141 aacacaggag aatccgatgc acacctatca atcaacaggc ccattcacaa taagcttaac
    25201 aattgccaat gacagttgca gttacacata cagttttccc cctaccaact ttggaagtgg
    25261 tggtacattt tatgatgaaa accatgatac aacacaattc accatgcatt atgatggctg
    25321 tgcaccattg accataagtt tcaataaccc ctttttaaat gttgttggtt cccattggga
    25381 ttttggtgat ggcaatacgt catctcaaca aaatccagta cacacctaca acgtaccagg
    25441 catttacgat gttatgctta ttgcaaactt atccaatgca agtgttgaca caatttatca
    25501 ttcacaggca gtaaaagtgg ccggaacccc tgttacattt acagtaaatt ccgtcaacac
    25561 ctgtaatggc tcaaacattc aggctttacc atcaaaccca aatttgcaaa gttataattg
    25621 ggattttggt gataatcaaa caagcacaca ggtttcaccg gtacatagtt atgccaatac
    25681 cagcaattac attatttctt tacaggctca ggatacaagt ggatgtacag tcattcattc
    25741 caagagctta tatgcaaatg ctgatgccaa tgctattctc tcaaataaaa acagagcttg
    25801 tgcatctgac accattacct tttcagttca aaacacaaat tacgtaaatt atacatggga
    25861 ctttggcgat ggcacgtcag caaccggtaa tccaatatca catatttact ctgactccgg
    25921 aacttattca gttcaagttg ttattacgga tataaatgct tgcacacaga caataaatgc
    25981 agtgcaacca attaacatag taaaacctgt ggctgatttt agcatcaata tgctatcttc
    26041 atcatgcact atggtaaatg tgcaactaac aaatacttct acaggaagca cttcttatac
    26101 ctgggattta ggagatggga ctatgtcgtc atcaactgac gtaaacaaat attactatta
    26161 cgggccaaat ccaccttaca cttatgatat taaactaaca gcatacaaca atggatgttt
    26221 aagcaccaaa accttagctc aggcagtaac cataccaaac tttgatgtta aattcagtta
    26281 tactagaagt caaggttgct taccaataac aattaatgta actgattcaa gcctaaatgc
    26341 tgtttcctgg aagtggtctt ggggtgatgg agacaccacg tttacacaag cctcacaaca
    26401 tacatttacc actgtaccac aagataaaat aaaactcttt gccaaagatg ttaatggctg
    26461 tgaaaaaaac tatgaactag ataacattca acttcctgca gcagtattta tgcagtcaga
    26521 tacaatagga tgcaatccaa aacaaataca atttactgat tcaagtaaaa atgcatatca
    26581 atggttatgg aaatttggta atggatcaac atctacagtt caaaatccag gagtcaatta
    26641 taatagtacc ggcaatttta cacctacttt aataattact gattcatttg gatgtaaaga
    26701 taccgttgat ggaaaaacca ttgcaatcac agagcctcag gctgatttta gttacacaaa
    26761 atctgcaggt tgtattcctg tgattgttca atttcaaaac gcatcttcag gtgcaacaaa
    26821 ctatttgtgg aattttggtg atggaagcac atcaacactc cagaatccaa accatattta
    26881 tacaactgtt ggaaactatc aggtaacact tacagtaact gatgcatcgg gttgcagtaa
    26941 cacaaaaact ttgaccaatg ctttacacat acctgggcca ttagcctctt tctcactttc
    27001 caacaattat ggctgccaac cattaactat cagccttaac gactcttcgc agaatgcact
    27061 aagtcatttt tggagttttg gagatggaga tacctcagtg aatgcttcac caactcacac
    27121 ttttacaaca gcaggctcct acttagtgac actcattgtt aaagatgcga atggatgcca
    27181 atcggtattt acacatccgg atacaattaa agtttacaat aaaccggttg cagcattttc
    27241 agtcagcgac tcttcaattt gtgtaggctc ttcaatttct ttgctcaatc tatcacaatc
    27301 atcaagtagc tataaatggc aattaggcga tggcagtacg tcacagcatt acaatgttaa
    27361 tcatccatat cagactccgg gacaatttag tattacatta gttgcatcta atggctactg
    27421 ttcggataca gcgaacagca tcaatgccgt taccgtaatt ccatttccaa tagctgagtt
    27481 tggaggttcg ccattgattg gctgcatacc actctcttca agcatggtta atataagcac
    27541 atcactacaa aatccggttt ttatgtggga tggtggcaat gggcttactt catcagcatt
    27601 caatccgcag tttaactatc aaaatgcagg atattactca gtgacattaa aagtaactaa
    27661 tgaaggactt tgcagtgata gtattactaa actcaacttc attgctgcac aaaacaatca
    27721 acctattacg ccttcagaaa taagctacat aactgtactc gatagtaatg caatcagaat
    27781 atcatggcaa ccctcatcag ctaccaatct gaatagattt gaattataca gatcaactga
    27841 caataaaaat tacaacctga tttacacaga ttcaaattca aacaacagca cattgaatca
    27901 gcaattagcc tatattgaca acaatgtaac aaccaatcag aatcgctatt attacaaaat
    27961 tctgactatc aacaactgta ataattcata tccattggat agttcaagag cccatgcttc
    28021 aatactcttg acaacagcac cagacaccat gtcaaataca ctttcctggt taccttatat
    28081 tggtgcgcca atttcacaat attctattta ccgcagtatt gatggattga attatgacct
    28141 gatagatgca attgcaggga cagaagattt ttatattgac tctacagcat attgcccgca
    28201 ggaatacagt tataaaatag aagcgcaatt agacggcagc atatacactt ccgaaagtaa
    28261 taatgatgca tctgtgccaa caagtcttgt aaatcaaata accacatcag tcatacgatc
    28321 aacagtgatt gacaacaaca gcattttaac agaatggtat gaaggcacac catttcctga
    28381 gttggtttac tattatttaa tttacaaatc gtcagataaa aatccaggca attttatttt
    28441 gattgactca gtaaacaaca gcacaaaata ctaccttgac agagatgttg atgtcaacac
    28501 tttcacctat caatacaaaa ttgaagttga aaatatctgc cagcaacgaa caggcctgac
    28561 agatttatca ggaaacattc ttcttcaagg taaattagac gacatgaaca acagtagact
    28621 tgaatggacg ccttataaag gctggaaaga aggtgttgaa cgctatgaaa tacaaaaaca
    28681 agacgaaaac ggaaattgga aaaccataaa atccgtagat ggtaataccc atcacatgga
    28741 agaagaataa ttttcagaag ttcaaacaaa attctgcaac ctttttctcg catttgcata
    28801 taaacactat aaacaatcgg catgcagaat aaaaaaatac tcataacagg atatagccaa
    28861 tgcggcaaaa gttctattgt gaaccgattg gtgaataaca gatttcatca ctcctattca
    28921 gaagacaatc aattatgtat tgcaagaaag gaattggcaa taaacaaaag aaaagtcaat
    28981 cttgtaatct ggacagcacc cggaagtgtg tcgaatctaa aatcattcaa gtcatattac
    29041 cttggaacag atgccttgat acatgtagtt gatgtaaaca aaccatcaac attttcagat
    29101 cttgatgcat ggattagtca ctatcaaaaa tttcttcccg gaacgcctgt ttatttaacc
    29161 tgtaacaaaa ttgaatcaaa tataaatctt gactgtagcg atttctttac tcagttcgac
    29221 tgttacagaa ccttttttac atcagcaaaa aacaatttca tgattactga aatgtttcaa
    29281 acgatagctg ctgatttact gagcgaaccc ttctatcaaa aaatcaaaat ctactgatat
    29341 gaaaaacgtt cacagcaaaa gccagattat aatttttgat aactatggca acattcttaa
    29401 aagctgtgac acaatttttc aggcaagtga tttgataggc aaaaacatta tgaaacaatt
    29461 tcctctgctg aattgctatg aacctgaaat cagaaatctt gcaaaaggca atgaaccgtt
    29521 ttttgttcct tgcatagcat tctcatttgc taacttcaaa agtatttgtg actttgtttt
    29581 tgttaggcat gaagaaatga atgaaataac catatcatgg atgatttcag acaattatgt
    29641 tcattttaaa gaaaggttga acaaaccgat caatcttcct caaaatagaa ctaaaccaat
    29701 ggtcacagaa tttgtcaacc gttggcatag aaaataggta gcatttaggc ctcaattaat
    29761 tctttctttt ccagataata taaaaaagtc tttacaactt tgaagcctgc atcttgcaat
    29821 acttctttgt aattagctat ctgatgaacg tgatcagctt caattgcgcc tgttttgtaa
    29881 tcaacaacta tagccttatc cccttcaagc ataagcctgt ccggtcttaa aatatttcct
    29941 tctgcatctg ccatttcatg ttcattataa atttgatagt atgggctaac ccatttcttt
    30001 tcagagagaa atttgcaggt agccttaact ccattcaaca actcattctt ttcttcatca
    30061 ggtatctgaa ggttacttaa atgattttct attttttcaa gatcagcttc actgcgaaaa
    30121 tcagccagca actcatgtac tttaattcca atgagtcggc ttttactttg ttcagcaaat
    30181 tttttaattt tgaatccggt gcttatcctg ttttctcttg cataataatt tatcagttcc
    30241 ttatgagtaa aactatcagg cacgtctgct gccttctttc gcttatgtat atatttagaa
    30301 tcaactccca aggtgaaaac agtatcactt gcttttgtca aaactgagct aacagtatca
    30361 gcattaatta ttgccgactt aattaatgta ttgactttac taataccgtc ctcactttct
    30421 tttccactat ccgaaatcag aataaataat ttatcttctg ccctggtaca tgcaacataa
    30481 agtgtattta tttcttctaa tacctttcct ctgttttcaa gaatataagc ctctctaaaa
    30541 taagtattta ataacttttt tgaaggcaag acaggaaatg cacctaattt gtcgaacggt
    30601 gaaacatcac ttcttatcca tatcaaacct ttactactat ttaaatccca atctgcatat
    30661 ggaatgatta ctatggggaa ctgtaatcct ttagacttat gaatagtgat aatggtaaga
    30721 gcattttcat cagcaggaac ctgcactgaa attttatttt gattggactc cacatcccac
    30781 caatcaagaa actcagcaac agtattaatc cctttatttg atttttgaaa aatcaaatct
    30841 tcaaaacatt gcaggtatgc atcaggttgc tt
//
LOCUS       DAOQNP010000028        28708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_519_length_28708_cov_9.050117, whole genome shotgun sequence.
ACCESSION   DAOQNP010000028 DAOQNP010000000
VERSION     DAOQNP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28708
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_28708_cov_9.050117"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..992)
                     /locus_tag="PKY09_05650"
     CDS             complement(36..992)
                     /locus_tag="PKY09_05650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNB12375.1"
                     /translation="MCRSFALLFLMVSISVVIPTYNRLNFVVKTIESLLNQQSDDFEL
                     LIIDDGSTDKTEEFFASYKHPKVTYHRIVNSERGFARNYGASIAKGKYVNFFDSDDLA
                     YNNHISTALHYIEKFDNPEIFHLNYVYQTLDGQELDVLKADSDNIGEMLIRKGNILSC
                     NSVIIRKDITEKFPFCESRLLSGTEDYALWLALSSRFKIHYVPEITSVVIDHEQRSMA
                     SHDIIKMINRNMFLLDHLQSDQHFIQYVGKRFNQIQSECFSFIALHLMLEGKNRDGVY
                     FLSKALMADWRFPFRRIRFMAIMKHMLKSFFRIPKNKVPETI"
     gene            1094..1663
                     /locus_tag="PKY09_05655"
     CDS             1094..1663
                     /locus_tag="PKY09_05655"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNB12376.1"
                     /translation="MNHRPTEFTERKKGAIEVICGSMFSGKTEELIRRLKRARIAKLK
                     VEIFKPSVDQRYHESNIVSHDSNYIPSSPVQSSSQILLLTNDVDVVGIDEAQFFDLEL
                     PNVCLKLAHQGIRVIIAGLDMDFKGEPFGPIPRLMAIADFVTKVHAICMDCGDLALFS
                     YRTVPDESLIVLGEKESYIPLCRTCYHKR"
     gene            1723..4224
                     /locus_tag="PKY09_05660"
     CDS             1723..4224
                     /locus_tag="PKY09_05660"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNB12377.1"
                     /translation="MYTITEAASILKGTLVLNENDNQTFRYLLTDSRQIGVAGESLFF
                     AIKGDRHDGHLFINDLIRKGVKNFVVSDISISQNKEANFIVVENTLDALQQLASFHRK
                     KFNLKTIGITGSNGKTIIKEWLYQLLQPDFNIIRSPKSFNSQTGVPLSVWQINNEHNL
                     GIFEAGISTSGEMQKLEKIIEPEIGVFSNVGEAHSENFTSLDVKIQEKLLLFQHSKTL
                     IYCKDYSGIHNAVMQSEYFKNGLKCFTWSRRIKSDLQIGRIEKQNNSSTIQGIFQQQF
                     IRIEIPFTDDASIENAIHCWAMLLFLEIEQEIIAERMMLLSPVAMRLEMKQGINNCSI
                     INDSYNSDLGSLQVALDFLNRFQQHNNTGVTNTVLRKTVILSDILQSGRNEDQLYGEV
                     AEMIHSKKIDRLIGIGPAISRHADLFKTDKAFFASTEKFIEQYSSGLFANEIILLKGA
                     RVYGFEHISRLLQQKGHETILEINLTSMIHNYNFYKAKLKPETKIMCMVKAFAYGSGS
                     YEVANTLQFHRVDYLAVAYADEGIELRKNGITVPIMVMNPQEQSFDKMIEFHLEPEIY
                     SFRIMSKFSESVKRNRTYNQNPFPIHLKMDTGMHRLGFEADDLNELAVRLKNSKHLKV
                     ESIFTHLAGTDEAALDFFTQEQFQRFRDMSDTLKRHFQYNVLEHVLNSSGISRFPEMQ
                     LSMVRLGIGLHGIGTPSEQRQLLNVASLKSTISQIRNVRKGESVGYNRKAILDSDKVI
                     ATIGIGYADGINRKMGNGKVSFLVNGRFAPTVGNICMDMCMLDITNCQAREGDEVIIF
                     GNGLPVAELAAKTETIPYEILSVISQRVKRIYFQE"
     gene            complement(4225..5496)
                     /locus_tag="PKY09_05665"
     CDS             complement(4225..5496)
                     /locus_tag="PKY09_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNB12378.1"
                     /translation="MLRRVLIFLLIVFLIDLYVFQGIKVLIKDLTPSMSRLVSGIYWF
                     FTVFTFSVFIAGLITDWHTWPKAVRVYLFAAVMIILITKLFVVLFLLIDDLTRLVRWG
                     FSGLYQRFYDSPQIVNTEDKIVVSRSQFLVRMAFVISSIPFISLLYGMGDGKYNYQVK
                     RRKIKFRKLPSAFEGFKIIQISDIHTGSFFDTKHIEKAVAMINSLKGDVVFFTGDLVN
                     DRSDEAVPFMDTLSKINAKHGVFSILGNHDYGDYVQWPSQEAKIKNLEQLKNIYKEMG
                     WKLMLDEHTFISEGNDKLGLIGVQNWSTHMRFPKYGNLPKAVQGMDFAPVNILLTHDP
                     SHWHGEVTEKYPQIDLTLSGHTHGFQFGVEIPALKIKWSPVQYVYKEWADLYDDANGQ
                     HLYVNRGLGFLGYPGRVGILPEITIIELTQG"
     gene            5572..7620
                     /locus_tag="PKY09_05670"
     CDS             5572..7620
                     /locus_tag="PKY09_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB12379.1"
                     /translation="MKDTARPTNKLIHEDSLYLQQHAHNPVHWLPWSAEAFEKAKSEN
                     KLLIISIGYSSCHWCHVMEKECFENVAIAEVMNENFVCIKVDREERPDIDAVYMTAVQ
                     LMKGQGGWPLNCFTLPDGRAIYGGTYFNPQQWIQVLEQLSTMYQEDKSKFLEYASSLQ
                     EGMLQLEKTEITNIKPDFDILKNSITKWSSQFDKIYGGLQRAPKFAMPVNLNFLMQYG
                     HVCNDTEVQKHIRLTLSKMYNGGIFDQLDGGFARYSTDNRWEIPHFEKMLYDNAQLIS
                     VYAQAYRAFNDITLKNAAEETLNFLNERMYDKRGFYYSAIDADSEGEEGKYYFWTKAE
                     IRATINQLQLEDISKEKAFDIACCYFSIPDSKKENDEIVLQCNNTIFNDSSLGIIDSN
                     QLKELIDKIKSALKMVRNKRIHPVCDKKLITSWNALMISALADAAINFNNKEYELQAI
                     TTFINLEQNLYSDKGQLLHSSGTKQNNNYHLEDYAFLIQACIKLFECTWDFKWIEKTI
                     PLTNYAIQYFSHDDNVLFYDYTAHDTILKFRKTELQDNVIPCANSVMANNLFLLSKYL
                     NKEDFLLRSKKMLEKVIEQIPNYGFGYANWVLLLQRINQPFYEVAFGGNSAFMNKNKL
                     NEHYLPMKITAPIILKDKIEFTADKNVIDDLIYVCRNHSCLLPTSDISKVVEIITNQQ
                     "
     gene            7617..8057
                     /locus_tag="PKY09_05675"
     CDS             7617..8057
                     /locus_tag="PKY09_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12380.1"
                     /translation="MKLQKLILGVIFSIAIYGCQIFKINKSDKHGNRQGYWEHYWNDE
                     GDIQRKGKYKHGLEVKKWKYYDLSGKLIKQEKYNKKRDVIDIKTYYENKKIKSNGQAF
                     LINENDSILHFYWQGPWKYYSPNGVLEKTEWYEKGKIKKAVTYP"
     gene            complement(8084..8578)
                     /locus_tag="PKY09_05680"
     CDS             complement(8084..8578)
                     /locus_tag="PKY09_05680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB12381.1"
                     /translation="MAEEILNMTATTFENPSQSNGTMDVFPINYSEKHIAELIHEIRR
                     KVFVLEQHIDPMIEFDTFENESQHYLAYVKNVPAGTARWRHTDEGVKLERFSVLKEYR
                     NSGLGRMLVNRVLCDVKLLGKKVYLNAQTTTVRFYEKAGFKVVGELFYEANIPHFKME
                     LVTS"
     gene            complement(8590..12861)
                     /locus_tag="PKY09_05685"
     CDS             complement(8590..12861)
                     /locus_tag="PKY09_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12382.1"
                     /translation="MLLFINISVNAAVTVSPATSGNCLNITPASFLPIGDIVITEGSN
                     GDFSIQSNKTLILSAPAGFMFQPGSGNVSFLASRNITAASISVTLTTITVTISVSGIP
                     KADELIISNIYARASTSNVSGNILRKSPGGGTATISGNAAGAGINHGTLSCAGTGQTV
                     TSVANGSWSNPATWQGGKIPLCTDNVIIYHQINVDVVTPALTSLIIKAGAKLIANLPV
                     IVNSNFTIEANAWYVHNNNSNAATTIFNGTESIDDNATIEVNNWSAAAVPFMTGISST
                     VGNLILNYNAVWQQDGLFAPSKIKGNLSVTNGQVVMDDGTGMSTLLNLNHVSIYKTGA
                     IIFAQGANRNLTLNTLSFTDSSKSNALTALMFRTYGTLEWNNAGSVYLSHDFSAVEGT
                     AGYHAFGATVNIGGDLSVVKGKVDFFRYVDGNGVIQVSGNTYCNLTAGNWFRLADYNT
                     VNLDFTTNNLSISGTESCYLQGSDGSTNFNINGSFSYTASSLFVFINNFTNSALQQIN
                     VSKDFFVTSGNVGLAFTSGKLNTHISGNLSVSGSSTQLVSQFYPYGTDSVIFVIDGNC
                     SLSGGMFNVSWNRGPVNFISNGIFNQTNANFEVVFHPFPGNYAACSVFIDSLNFSGGT
                     FKVFDSYITDGKTVSITTGSDVNINFQNSSDRFVLISNTLWWNNPALNLTINGDLKIS
                     GNNSAIFASNVGGGNESIVINGNFDILGGVNSIGGSTSLGSKQHDITVSINGSLTQTG
                     GQFYYSINEGNVDYNILNDLIQTGGMQNLTYQDAAAKLQIGGKYLQSGGTFNFHGSTG
                     NTSLADSVIVNGNFSQSNGVLRFDNAQGSLNMSQNVVVLNSDSVLFSGNGVITHANHL
                     TTNTLFGKILYNKTGTSSFQRATSTHDLQQVEMVVGPLTTLDVKSSIQPLLIASHASS
                     SPAVNNVFTINGVLDMGLQRIMARQQGNYYSQLSVGSTGKIKTMNPNGLYSISTASTI
                     NTLIAGNNRMNFWLDAQSTIEYNGNQNQNITGIPNGIANSSSNKYGKLQIDFAGVNNI
                     NFVKPEADSAVFVRSALVLTSGELNLNADHVSNTNGLSIVLENGCQIFRASGYIRSET
                     IDGSAAVHLSPQQNAGYEIPFGYNSTAYIPFKYQCTNGCDWVSLATYRSLPDNTPFPP
                     GVTHVRSISGADNSLQTIDRYWYVNAVGNPNANLKFTYAPIEKGSISTPRAQRWIGND
                     AWESFKGMQSNPTPYETEVNGITGLNSWWTLSSQSSPLPVEILSFNANCQSQRVVLNW
                     SVATQLNNDIFILERSRDGKKWENLTSIKGAGTTNTLMNYETKDEAPYAEVTYYRLAQ
                     IDYDGKRNELKTIAVKGCGSDPVRMLYYSFTDHKIVIKIQSSYNDKIDIFLFNENGQT
                     VARKVQNIDNNIQEIQMECPQLNSGIYMLSIRGSFNNIIKKLVKN"
     gene            complement(12975..13898)
                     /locus_tag="PKY09_05690"
     CDS             complement(12975..13898)
                     /locus_tag="PKY09_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB12383.1"
                     /translation="MPVNHQEVQSLSHLELLAKQVVEGFITGLHKSPFHGFSVEFAEH
                     RLYNTGESTRHIDWKLFGRTDKLFVKRFEEETNLRCHIVIDNSASMHYPEVKGKKDLQ
                     NKVTFSVYSAAAILYMMKQQRDAIGLTVFSEQIDVQTQAKSSTAHHKLVFAHLEKLLE
                     NKADLNRKTATADALHQLAESIHKRSLIVIFSDMFENMDSNEALFKSLQHLKHNKHEV
                     ILFHVTDKKHELDFQFENRPYRFVDVETGEEIKVHSNMVREKYIEAMKEYKSMLIHKC
                     AQYKIDFVQADIHEGYHQVLLAYLLKREKLH"
     gene            complement(13923..14243)
                     /gene="trxA"
                     /locus_tag="PKY09_05695"
     CDS             complement(13923..14243)
                     /gene="trxA"
                     /locus_tag="PKY09_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNB12384.1"
                     /translation="MAIEITDSNFEEVVMKSDKPVLVDFWAEWCGPCRMVGPIVEELA
                     KDYEGKAVIGKIDVDSNPNVTMQFGVRNIPTILFFKNGQVVDKQVGAAPKNILAKKID
                     TQLV"
     gene            complement(14402..17992)
                     /gene="dnaE"
                     /locus_tag="PKY09_05700"
     CDS             complement(14402..17992)
                     /gene="dnaE"
                     /locus_tag="PKY09_05700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNB12385.1"
                     /translation="MPRFSHLHNHTQFSLLDGAADIKSLYKKAVKDNMPALAITDHGN
                     MFGVFNFVAEAAKYNTKENPNVIKPIVGCEFYLVENRHKRSFTKDDKDVRYHQLFLAK
                     NNIGYKNLVKLTSLGYMEGLYGKYPRIDKELVLKYHEGLIATTCCLGASVPKTILRKG
                     EAEGEREFLWWLDIFGEDYYVELQRHDIPEQNTVNEVLIGFALKHNVKIIASNDSHYV
                     NREDYNAHDILLCINTGEKQSTEKFKDGDEDTSARDKRFAFYNDEFYFKDTSEMQQLF
                     SDRPDAIDNTNEIVDKIEPLKLKQDILLPHYQIPQGFENQDHYLKHLTWEGARKRYSE
                     ITPDIEERINFELFTVKTMGFAGYFLIVADFINHGKNIGVLVGPGRGSAAGSAVAYCI
                     GITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIDYVVDKYGKNQVAQIIT
                     YGTMAAKMSIKDVARVLDLPLPDSNALAKLVPDRPGISLGRLLTAPIDGEKSLKTKEN
                     LSADELEKVKSLRSILEEGTSLQSSVLKEAMVLEGSVRNTGIHAAGIIIAPTDLTDII
                     PVSTSKETDLLITQFEGKIIEDAGVIKMDFLGLKTLTILRDAIRLIEKNHGVKINLDE
                     IPLDDSRTLALYQRGETNGTFQFESVGMQKYLKELKPDMFEDLIAMNALYRPGPMEYI
                     PNYIARKHGKEKVTYDLPEMQEHLQETYGITVYQEQVMLLSQKLGNFTKGDADTLRKA
                     MGKKQIEVLNKMKNKFMDGAKGNGHDEKVLEKIWSDWEAFASYAFNKSHSTCYAFVAF
                     QTAYLKANYPSEYMAAVLTNNLNNLDKITFFMDECRRMNIPVLGPDVNESELQFSVNK
                     KGEIRFGLAAIKGVGEAAAESLLTERKKGGVFTDVFDLVSRVNLRAANKKCFENLAMA
                     GALDFDTSFHRAQYFAIDPKDNQTQLEKIIRFGSANQEAKNSTQHTLFGDLGGVDASK
                     PKIIPAEHFSNLEQLKKEKEIIGIYLSGHPLDDYKEEIKTFCNISVEQLADLKQLNNR
                     DVNFAGIITNANHRVSKTGKPFGSFIIEDYNASFEFAVFSEEYAKFKHLLNNDAFVFI
                     KARVQTKFYNENEFELKIISVHFLDEVANKMAKGIMISLRSESINEMKIKKLKEISKN
                     SKGKLTVKFSIRDNDQNLSIDVESNQLKLEYNKELFKELNLISERVSLIK"
     gene            complement(18295..19170)
                     /locus_tag="PKY09_05705"
     CDS             complement(18295..19170)
                     /locus_tag="PKY09_05705"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNB12386.1"
                     /translation="MNKPKGKLIAIGGNEDKGTESEPNFEQKNNLNFFALQILSRICH
                     EMGGKNAKIEVITSASSIPVEVGGNYIDAFGKLGCTNVNVMDIRNREDATKPEYIERI
                     KKCDGIMMTGGNQLRLSTIFGGTEILEILQHRYQHENFVIAGTSAGAMAMSNTMIYQG
                     SSSGALIKGEVKITTGLALMGDVIIDSHFDKRGRFGRLAQAVASNPSCIGIGLGEDTG
                     VLLSEGYLLEIIGSGLVILIDGHHIRHNNIADLQEGVPISIENLIVHVLTKGNCYDLK
                     SRKFYSELIKESKPA"
     gene            complement(19336..20637)
                     /locus_tag="PKY09_05710"
     CDS             complement(19336..20637)
                     /locus_tag="PKY09_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB12387.1"
                     /translation="MLSKKIILIVLAGWMPTIVTAQQNINSDLKTLIQKSIAYFPKLK
                     ETELGLQAGKEKLEIAKSAYYPNIMLNAGYNYIDPVSSVSFPVAPGVIKAMQFQPYNN
                     YTATAGFTYPVIDFGRAWFFVRKSNEELEQSKLNIELNKAQLAAQVASVYYSMAYFSK
                     AVAIEDTVLLAMHSYQNVIISRLRNGDALILDSITTGSAISEEENRKLSLQNLLSKQI
                     ALLQYTTNETSIVMPEKFDFPQWYLESDTTNQVQSSFEYKIISSKLKAQQFDIKMNRS
                     NLFPVVSVNAGLGYRNGYQPDIEKLVFGTQAGLSISAPIFYGNKARNSIKLSKLYLKQ
                     LQQSQENVSHSFRKDISNVRSDISTLEAQIVNSNRQVLQSRSAVELAQSRYRNGLVTY
                     TDVLNALANRQRAELSKLNLEYQLCLARIELARITGVAYYE"
     gene            complement(20646..22235)
                     /locus_tag="PKY09_05715"
     CDS             complement(20646..22235)
                     /locus_tag="PKY09_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNB12388.1"
                     /translation="MTEPASYPSGFKRIVIVLTTISAAIMELIDTSIVNVGLTDISGS
                     LGATIEDTSWLITAYAIANVIIIPLTGFLAKYFGRKNYYLASTVIFTAASYLCGESDS
                     LIELIAWRFVQGIGGGALLSTSQSILFDAYQPEKRAMASAMFGMGIVLGPTIGPTLGG
                     YIMEHSSWPLIFFINIPFGIVAVLLTWLYIDRKPEEININRKEIHIDYTGILLLAIGV
                     SSLQYVLERGQTEDWFNSRDIIYLSILSVFGIGFFIWHELRHPHPAIDLRIIKNRNLF
                     ASNVLTFVCGFGLFGSVFIFPVLVQRVLGYTPMEAGLGIVPGAIAAICMMPFIGKGLS
                     SGIRPIYFVMTGFIVFILHGYVSSLANLDASREWFIVPQIMRGVGTSFLMVPLINQAV
                     IGISPQKMPYAISLTNMLRQLGGSFGIAIMNTYVAQRVAIHRTDLVANLTANDPETTE
                     RLKQVAAFTMSKGFSPQQATQAGYQILEGTLTKQSYLHAYLDGFLLISVFLICAMPFI
                     FLLDTKKQSAATLAKVAEEAH"
     gene            complement(22232..23272)
                     /locus_tag="PKY09_05720"
     CDS             complement(22232..23272)
                     /locus_tag="PKY09_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNB12389.1"
                     /translation="MESPEQDKKKFNPLFVIAIVVIAIAAFLGIKNLIHNLRYEITDN
                     AQIEAAGIPVISRISGYVDSLSITDFSQVKEGMPLVSIDSREFILAEQQAEADVQSAE
                     AELLNANAGKKNAEAALQVAQTNKELQKIKTDKAIADYNRDKALFNDGSITQKQIDDS
                     RINMEAMLKSLDVTSDQIKVAMAQQETAVSMVKRAEALKETRKAMLDQAKLRLSYCSI
                     SAPATGKIGKCNINIGQFLQAGQTLFTIVNSEKFWVVANFKETQLKSLKEGGEANLIV
                     DGFPDQKITGRIASLSEATGSRFALLPPDNATGNFVKVTQRVPVKIAIENFSEYKNML
                     RAGMSVTVEVKK"
     gene            23352..23825
                     /locus_tag="PKY09_05725"
     CDS             23352..23825
                     /locus_tag="PKY09_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNB12390.1"
                     /translation="MAGKKKDITQYARFNIGRLISIYKRAFDEWAMAGLAERGYPQFK
                     ISYFAVLMNINENGKTNKEIAAAAKVSKQAMSRIVKELVSMKLIQVTTHPDDNRSALL
                     KLTTKGRTMVNETKEVMLQLESHYEKAIGKKEFLNYKETTAKLTNLHWPEFYPHH"
     gene            23914..24456
                     /locus_tag="PKY09_05730"
     CDS             23914..24456
                     /locus_tag="PKY09_05730"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HNB12391.1"
                     /translation="MIRQAICLIALITYATSTSAQELIDWQEKRLTWDDFEKRNLPHS
                     RTGAITSSGIYFSYIEHNDGLDIIVKAQMDKSRSWVNTHSLRDEVLSHEQVHFDISEI
                     HARLLRQYFALNNRKSAGRNPESIYKKYINRLRRMQDDYDRQTNHGNDLYMQMMWEEK
                     IKAMLIDLEQYSGQEMAWRQ"
     gene            complement(24532..26904)
                     /locus_tag="PKY09_05735"
     CDS             complement(24532..26904)
                     /locus_tag="PKY09_05735"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNB12392.1"
                     /translation="MYVIKRDGKKESVKFDKITARIQKLCYGLDPHHVSSLNVAQRVI
                     EGVYDGVTTSELDNLAAEVAATMTVKHPDYALLASRIAISNLHKNTKKSFSETMKDLY
                     TYIDPKTGKLAPLLADDVWAVIEKNAELLDSTIIYDRDFGYDFFGFKTLEKSYLLKLN
                     GKVVERPQHMLMRVSVGIHKEDIEGVIETYNLMSERWFTHATPTLFNAGTPKPQLSSC
                     FLLTMKGDSIDGIYDTLKQTALISQSAGGIGLSIHNVRATGSYIRGTNGTSNGIVPML
                     RVFNDTARYVDQGGGKRKGSFAIYLEPWHADIFEFLDLKKNHGKEELRARDLFYAMWI
                     PDLFMKRVEAEGEWSLFCPNECPGLADTYGAEFEMLYTKYENEGRQRKTIKARELWSA
                     ILESQVETGTPYMLYKDACNEKSNQKNLGTIKSSNLCTEIIEYTAPDEVAVCNLASIA
                     LPRFVNNGAFDHQKLFDITYVATKNLNKIIDINYYPVPEARNSNLRHRPIGLGVQGLA
                     DAFILLGYPFESEEARLLNREIFETIYYAALTASKDLAKVNGPYSSYEGSPISKGEFQ
                     FDMWNVKPTDRWEWDVLREEILKHGVYNSLLLAPMPTASTSQILGNNECFEPYTSNIY
                     TRRVLSGEFIVVNKHLLRDLVKLNLWNSEIKNKIVAANGSVQDIAEIPEQIKEVYKTV
                     WEIKQRAVIDMAADRGAFIDQSQSLNLFIQDPNFAKLSSMHFYAWKKGLKTGMYYLRT
                     KAATDAIKFTVEGHSNHNIVATTQSVSKEEAMAEISCSIDNKDDCLACGS"
     gene            complement(27032..28003)
                     /locus_tag="PKY09_05740"
     CDS             complement(27032..28003)
                     /locus_tag="PKY09_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase small
                     subunit"
                     /protein_id="HNB12393.1"
                     /translation="MIQDELLLRENKERFVLLPINYPKVWEMYKKHEQSFWTAEEIDL
                     HPDLVDWENKLNNDERHFIKHVLAFFAASDGIVNENLAVNFMSEVQIPEARCFYGFQI
                     MMENIHSETYSLLIDAYIKDPKEKQYLFNAIETVPCVKKKADWALRWIQNGSFAERLV
                     AFAAVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSMLQNKM
                     TEEQVHTIIKDAVNCEHEFVTDALPVSLIGMNARLMCQYIEFVADRLLVSLGYSKIYN
                     ATNPFDFMEMISLQGKTNFFEKRVGEYKKAGVAQSQESQKIRFDEDF"
     gene            complement(28400..>28708)
                     /locus_tag="PKY09_05745"
     CDS             complement(28400..>28708)
                     /locus_tag="PKY09_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12394.1"
                     /translation="PVPVANIENSYAMVMKNTDLQTERKQDIIKDIALQKLNSITEDI
                     AGLPVEKGKLKTLAVFGKIVNRLTFKRVNIETTYSDDGRLMAYSVTAGNFNFEHEVVK
                     "
BASE COUNT         9140 a         5502 c         4827 g         9239 t
ORIGIN      
        1 ttaaggatgc taatgtaaaa ctttaagttc ttgttttaaa ttgtttccgg aaccttgttc
       61 tttggtatcc ggaaaaatga ctttaacatg tgtttcataa tagccataaa acgaattcta
      121 cgaaatggaa agcgccagtc tgccattaaa gcttttgaaa gaaaataaac accatctctg
      181 tttttacctt caagcatgag gtgcagggca ataaatgaaa aacattcact ctgaatctga
      241 ttaaaacgtt taccaacata ttgtataaag tgctgatcgg actgcagatg atctaacaga
      301 aacatgtttc gattaatcat cttaataata tcgtgcgaag ccatactacg ctgttcatgg
      361 tcaataacta cactggtaat ttccggcaca taatgtattt tgaaccttga tgacaatgca
      421 agccataaag cataatcttc agtacctgag agaagacggc tttcacaaaa tgggaatttt
      481 tcagtaatat cttttctgat tatgacagaa ttacaactta aaatatttcc cttcctaata
      541 agcatttcac ctatattatc agaatcagct ttcagtacat ctaactcctg tccatcaaga
      601 gtttgataaa catagttgag atgaaatatt tcagggttat caaatttttc gatataatgt
      661 agtgcagtag aaatatgatt attatatgcc aaatcatctg aatcaaaaaa attgacatac
      721 ttgcctttgg caattgaagc accataatta cgtgcaaacc cacgctcgct gttgactatt
      781 ctgtgataag ttacttttgg atgtttatat gaagcaaaaa actcctccgt tttatcagta
      841 ctgccatcat caataatcag aagttcaaaa tcgtctgact gctgattcag caatgattca
      901 atcgttttta caacaaaatt caacctgtta taggttggga ttactacaga aatggaaacc
      961 atgagaaaaa gtaatgcaaa actacggcac attaactgaa aacacacatt tttcaaaaaa
     1021 aaatataata taaatgttaa ctgactgaaa attgcagcaa acagatttta cctatttttg
     1081 aaaccactaa gttatgaacc atagaccaac agaatttaca gagagaaaaa agggcgctat
     1141 tgaagtcatt tgtggtagca tgttcagtgg caagacagag gagttgattc gcaggctaaa
     1201 acgtgcacgt attgccaaac ttaaggttga aatatttaaa ccttctgttg atcagcgtta
     1261 tcatgaaagt aacattgttt cgcacgattc aaactatatt ccatcttctc ccgttcagtc
     1321 atcctctcaa atacttttac tcacaaacga tgttgatgta gtaggcattg atgaagctca
     1381 attttttgat ttggaactgc ctaatgtttg tcttaaactg gcacatcagg gaataagggt
     1441 aattattgcc ggtttagata tggactttaa aggtgaacca ttcggtccaa ttccgaggct
     1501 tatggcaata gcagattttg ttacaaaagt acatgccatc tgtatggatt gtggcgacct
     1561 tgctttattt tcttatcgta ccgtacccga tgaatcactt attgttctgg gtgaaaaaga
     1621 aagctacatt ccattgtgca gaacttgtta tcataaaagg taattgttgt caatttgatg
     1681 cactatcaaa gcttaaattt gtaagcaatt caattttaaa atatgtacac cattacagag
     1741 gcagcgtcaa tactgaaagg aacgctggtt ttgaatgaaa acgataatca gacttttcgt
     1801 tatctgctaa cagacagccg gcaaatcggt gtggcaggag aatcactttt ttttgcaatt
     1861 aaaggcgacc gtcatgatgg tcatttattt attaatgacc ttatccgaaa aggtgtaaaa
     1921 aactttgttg ttagcgatat atctatttca caaaataaag aagctaattt tattgtggtg
     1981 gaaaacacgc ttgatgcact acaacaactt gcctcttttc atcggaaaaa atttaatctg
     2041 aaaacgatag gtattacggg cagtaatggc aaaactatta tcaaggaatg gctttaccaa
     2101 ttattgcaac ctgattttaa tattattcgc agcccgaaaa gttttaattc tcaaactggc
     2161 gtaccattat ctgtttggca aataaacaat gagcacaacc tcggaatttt tgaagcagga
     2221 atttcgacat caggtgaaat gcagaaattg gaaaaaatta tcgagcctga aattggtgtt
     2281 ttcagcaatg ttggtgaagc acatagtgaa aattttactt cacttgatgt taaaatacag
     2341 gaaaaattat tgttattcca acattccaaa accctgatat attgcaaaga ctattcagga
     2401 attcacaatg cagtaatgca atcggagtat tttaaaaatg ggttaaaatg ttttacctgg
     2461 tcgcgaagaa taaaatctga tctgcagatc gggagaatag aaaaacaaaa taacagcagt
     2521 accattcagg gtatttttca gcagcagttt atcagaattg aaataccttt tactgatgat
     2581 gcatctattg aaaatgccat tcattgctgg gcaatgttgc tttttttgga aattgaacaa
     2641 gaaataattg ctgaaagaat gatgttgctg agtcctgtgg caatgcgatt ggagatgaaa
     2701 cagggtatca ataattgttc aatcattaat gacagctata attcagattt aggctcactg
     2761 caggttgctt tagatttttt gaatcgtttt cagcaacata ataatacagg agtgactaat
     2821 acagtactac gtaaaacagt tatcctttca gacattcttc aaagtgggcg aaacgaagat
     2881 caactttatg gagaagtagc tgaaatgatt cattcaaaaa aaattgacag gcttattgga
     2941 ataggaccgg caatatcccg gcatgctgac ttatttaaaa cagataaagc tttttttgct
     3001 tcaacagaga aatttattga acagtattct tccggattat ttgcaaatga aattatttta
     3061 ttaaaaggcg caagggttta tgggtttgaa catatctcaa gattgttaca gcaaaaaggt
     3121 catgaaacta ttttggaaat aaacctgact tccatgattc acaactataa cttttataaa
     3181 gcaaaactca aaccggaaac aaagattatg tgtatggtaa aagcttttgc ctacggtagt
     3241 ggcagttacg aagttgctaa tacactgcaa tttcatagag tagattatct ggcagttgcc
     3301 tatgcagatg aaggtattga actcagaaag aatggcatta ctgttccgat tatggtaatg
     3361 aatcctcagg aacagtcttt cgataagatg attgaatttc atctggagcc tgagatttat
     3421 agtttcagaa ttatgagtaa gttcagtgaa tctgttaaac gtaaccggac gtacaatcaa
     3481 aacccatttc ctatccattt aaaaatggat acaggcatgc acagacttgg ttttgaagct
     3541 gacgatctga atgaacttgc agtgcgttta aaaaacagca agcatctgaa agtagaatct
     3601 atttttactc atcttgcagg aactgatgaa gccgcactcg acttttttac acaagaacag
     3661 tttcagcgat tcagagatat gtctgatact ttaaaaaggc actttcagta caatgtgttg
     3721 gaacatgttt tgaattcatc aggtataagt cgtttccctg aaatgcaact gagcatggta
     3781 cgtctgggta taggtcttca tggcataggc acaccatcgg agcaacggca attattaaat
     3841 gttgcttcat tgaagtcaac catttcgcaa ataagaaatg ttcgaaaagg agaaagtgtt
     3901 ggttataaca gaaaggctat attggatagt gataaagtaa tagcaacaat cggaattggt
     3961 tatgcagatg gcataaaccg gaaaatggga aatggaaaag ttagcttttt agtgaatggg
     4021 agatttgctc caacagttgg caacatctgc atggacatgt gtatgcttga cattactaat
     4081 tgtcaggcgc gcgaaggcga tgaagtgatt atttttggca acggcttgcc cgttgccgaa
     4141 cttgcagcaa aaacagaaac aattccttat gaaattctat cggtaatttc acaacgtgtc
     4201 aaacgtattt attttcagga ataattatcc ttgcgtcaat tcaattatcg taatttcagg
     4261 aaggatacct accctgccgg gataaccaag aaaaccaagt cctcgattta catacaaatg
     4321 ctgaccgttt gcatcatcat acaaatctgc ccactcttta taaacatact gaacaggact
     4381 ccatttaatt tttaatgcag gtatctcaac accaaactga aacccatgtg tatgacctga
     4441 taaagtcaaa tctatttgtg gatatttttc cgtaacctct ccatgccaat gtgaaggatc
     4501 gtgagtaagc aaaatattaa caggtgcaaa atccattccc tgcactgctt taggcagatt
     4561 gccatatttt ggaaatcgca tgtgtgtact ccagttctgt actccgatta atcctagctt
     4621 atcattaccc tcggaaataa acgtatgctc atcaagcatc agcttccaac ccatttcctt
     4681 atagatattt ttcagttgtt ctaagttttt aatttttgct tcctggcttg gccactgaac
     4741 atagtcgcca taatcatgat ttccaagaat agaaaaaaca ccatgctttg cattaatctt
     4801 tgatagtgta tccataaacg gtactgcctc atcagagcgg tcattcacca gatcacctgt
     4861 aaaaaaaacc acatcgcctt ttaaagagtt aatcattgca acggcctttt caatatgctt
     4921 tgtatcgaaa aagctgccgg tatgaatatc agaaatctgt ataatcttaa aaccttcaaa
     4981 tgctgaaggc aatttccgga acttgatttt tctacgttta acctggtaat tatacttgcc
     5041 atctcccatt ccatataaga gagaaataaa tggaattgat gaaataacaa acgccatccg
     5101 cactaaaaac tgcgaccttg aaacaacaat cttatcttca gtatttacaa tttgaggaga
     5161 atcataaaat cgctgataca atcctgagaa accccatctc actaatctgg taagatcatc
     5221 aatcagcaga aacaaaacaa caaagagttt tgttattaaa attatcatga cggcagcaaa
     5281 aagataaact cttactgcct ttggccaagt atgccaatca gttatcagtc cggcaataaa
     5341 aactgaaaat gtaaacacag taaaaaacca gtaaatgcca gaaaccaatc ttgacattga
     5401 tggagtcaaa tccttaatca atacctttat gccctgaaaa acataaaggt ctatcagaaa
     5461 aacaattaat aaaaaaatca atactcttcg caacataaaa ccgcgcaaat gtaagtgaaa
     5521 caattcatca atacctttct gtcatttaaa cttttctata tataattaac aatgaaagat
     5581 acagcacgcc caacaaacaa actcattcat gaagacagtc tgtatctgca gcagcacgcc
     5641 cacaatcctg tacattggct accctggtca gctgaagcat ttgaaaaagc aaagtctgaa
     5701 aacaagttac tcatcattag tattggttat tcaagttgtc attggtgtca tgtgatggag
     5761 aaagaatgtt ttgaaaatgt ggcgatagcc gaggtgatga atgaaaactt tgtttgtatt
     5821 aaagttgatc gtgaagaaag accggatata gatgcggttt atatgactgc tgttcaactg
     5881 atgaaaggac aaggtggctg gcctttaaat tgttttacct tacccgatgg gagggcaatt
     5941 tatggtggca cctatttcaa tccacaacaa tggattcagg tgcttgaaca attgtcaacg
     6001 atgtatcagg aagacaaatc aaaatttctg gaatatgctt cttcattaca ggaaggcatg
     6061 ctgcaattag aaaaaacaga aatcacaaac atcaaacctg actttgatat attaaaaaat
     6121 tcaataacaa agtggtcttc ccaatttgat aaaatatatg gtggccttca acgtgctcca
     6181 aaatttgcaa tgcctgttaa cctgaatttc ttaatgcaat atgggcatgt ttgcaatgat
     6241 acagaagtac aaaaacacat cagactaact ttgagcaaaa tgtataacgg aggcattttc
     6301 gaccaacttg atggtggatt tgcacgctat tctactgata atcgttggga aattccacac
     6361 tttgaaaaaa tgctctatga caatgcccag cttattagtg tttatgcaca agcttacaga
     6421 gcatttaatg atattacttt aaaaaatgct gctgaagaaa ctttaaactt tctgaatgaa
     6481 agaatgtatg acaaaagagg cttctattat tcagcaatag atgctgacag tgagggcgaa
     6541 gagggcaaat attatttctg gactaaggca gaaatcagag caacaataaa tcaactgcag
     6601 ttagaagata tatcaaaaga aaaagctttt gatattgcat gctgctattt ttcaattccc
     6661 gattcaaaaa aagaaaatga tgaaatagtt ctacaatgta ataataccat atttaatgat
     6721 agcagtcttg gaataattga ctccaatcag ctgaaagagc tcattgataa aataaaatca
     6781 gctctcaaaa tggtaagaaa taaacgcatt catcctgttt gcgacaaaaa acttatcact
     6841 tcatggaatg ccctaatgat ttcggcatta gctgatgcag caataaattt taataataaa
     6901 gaatatgagc tgcaagcaat aaccacattc attaatttgg agcaaaatct ttattcggac
     6961 aaaggacaac tacttcatag ctcaggaacg aaacaaaaca acaattatca tttggaggat
     7021 tatgcttttc tcattcaagc ctgtattaaa ctatttgagt gcacatggga ttttaagtgg
     7081 attgagaaaa caattccctt aaccaactat gctattcaat atttcagtca cgatgataat
     7141 gttttattct atgactatac agcacatgat accattctta aattcagaaa aaccgagttg
     7201 caggacaatg ttattccttg tgcaaattca gtaatggcaa acaatctatt cctgctttca
     7261 aaatatctaa acaaggaaga ctttttgttg cgatcaaaaa aaatgctcga aaaggtcatt
     7321 gaacaaattc caaattatgg ttttggttat gcaaattggg tattactttt acaacgaata
     7381 aatcaaccat tctatgaagt tgcctttggt gggaacagtg cattcatgaa taaaaataaa
     7441 cttaatgagc attatcttcc aatgaaaatc actgcaccga taattctaaa agataaaatt
     7501 gaatttactg ccgataaaaa tgtaattgat gacttgattt atgtatgcag aaaccactca
     7561 tgcctacttc ccacatcaga tattagtaag gttgtagaga taatcaccaa tcagcaatga
     7621 agctacaaaa actcatactt ggtgtaatct ttagtattgc aatctacggt tgtcagatct
     7681 ttaaaatcaa caaatcagac aaacatggaa accggcaagg gtattgggaa cattattgga
     7741 atgatgaagg cgacattcaa cggaagggga aatataaaca tggcttagaa gtaaaaaaat
     7801 ggaaatatta cgatttaagt ggcaaactaa taaaacaaga aaaatacaat aagaaaagag
     7861 atgttattga tataaaaaca tattatgaaa acaaaaaaat aaagtctaat ggtcaagcat
     7921 ttttaatcaa tgaaaacgat agcatattac atttttactg gcaggggcca tggaaatact
     7981 actcacctaa tggagtgtta gaaaaaaccg agtggtatga aaaagggaaa ataaaaaagg
     8041 ctgttacgta cccataacag cctttaaaaa ctctaaatat atgtcatgaa gttacaagtt
     8101 ccattttgaa atgaggaata tttgcttcat agaatagctc gcctacaact ttaaatcctg
     8161 ctttttcata aaatctaact gttgtagttt gggcattcaa ataaactttt ttaccgagta
     8221 gttttacatc acataatacc ctgtttacca gcattcggcc taagcccgaa tttctatatt
     8281 cttttagaac actgaatctt tctagcttaa ctccttcatc agtatgacgc catctggcag
     8341 tacctgcagg cacattttta acataagcca aataatgttg gctttcattc tcaaatgtat
     8401 cgaactcaat cattgggtca atatgttgtt caagaacaaa cacctttcgc ctgatctcat
     8461 gaatgagttc tgctatatgc ttctctgaat aattaatcgg aaacacatcc atggtaccat
     8521 tggattgtga aggattttca aaagttgttg cggtcatatt taaaatttct tcggccataa
     8581 aaatagaaat taatttttaa ctaatttttt tattatatta ttaaaggaac ctctgatgct
     8641 taacatataa attcctgaat taagttgtgg acactccatt tgaatttctt gtatgttgtt
     8701 atctatgttt tgcacttttc gtgccacggt ttgtccgttt tcattaaaca gaaaaatatc
     8761 tattttatca ttgtaagaag attggatttt tataacaatt ttatggtcag tgaaagaata
     8821 atataacatt ctgacagggt cggaaccgca gccttttacc gcaatcgtct tcaactcatt
     8881 tctctttccg tcatagtcta tttgcgccaa tctgtaatag gtgacctcag cataaggcgc
     8941 ctcatctttg gtttcataat tcatgagtgt attggttgtt cctgcaccct ttatggatgt
     9001 aaggttttcc catttttttc catctcggct tcgctctaaa ataaagatat cgttattgag
     9061 ttgtgtcgct acagaccagt ttaatactac acgctgactc tgacaatttg cattaaaact
     9121 taatatttct acgggcaaag gacttgactg actgctaagt gtccaccatg aatttaagcc
     9181 tgtaattcca ttaacttctg tttcgtacgg tgttgggttg gattgcatac ctttaaagga
     9241 ctcccatgca tcattaccta tccatcgttg cgcacgaggt gttgatattg accctttttc
     9301 aataggtgca taagtaaatt tcaaattggc attaggattt ccaacagcat taacatacca
     9361 ataacggtca attgtttgta aactattgtc agcacctgaa attgaacgaa catgagtgac
     9421 gcccggaggg aaaggtgtat tatcaggcaa actcctgtat gttgctaatg aaacccaatc
     9481 acatccattg gtacattgat atttgaaggg tatataagca gtactgttat aaccaaatgg
     9541 aatttcataa ccagcatttt gttgaggtga aagatgtact gctgcactgc catcaatggt
     9601 ttcacttctg atataccccg aagcacgaaa aatctgacag ccgttttcca acacaattga
     9661 caatccattt gtattcgaaa catgatctgc attaagattc aattcaccgg aagtaagtac
     9721 taatgcagaa cgaacaaaaa ctgcactgtc ggcttcaggt tttacgaagt taatattatt
     9781 tacacctgca aagtcaattt gcagcttgcc gtatttattt gaactcgaat tggcaatacc
     9841 attaggaata cctgtgatat tttgattttg attcccatta tactcaatag tactttgagc
     9901 atcaagccaa aaattcattc tgttatttcc tgcaatgagt gtattgattg ttgatgccgt
     9961 tgaaattgaa tataaaccat ttgggttcat tgttttgatc ttaccggttg aaccaactga
    10021 aagctgcgaa tagtaattac cttgctgcct tgccattatt ctctgcaatc ccatgtcaag
    10081 cacaccatta atggtaaaca cattatttac agccggtgat gatgaagcat gagaagctat
    10141 caacaatggt tgaatggatg attttacatc tagtgttgtt aacggcccta caaccatttc
    10201 tacttgctgc aaatcgtgtg ttgaagttgc tctttgaaat gatgaagttc ctgttttatt
    10261 ataaagaatt tttccaaaca aagtgtttgt tgtaagatga ttggcatgtg taattactcc
    10321 atttcctgaa aacaaaacac tatcagaatt taagaccact acattttgac tcatattcaa
    10381 tgacccttgt gcattatcga atcgtaaaac tccgttgctc tgactaaaat ttccatttac
    10441 aattacacta tcagcaaggg atgtattacc ggtgctacca tgaaaattaa aagtaccacc
    10501 tgactgtaaa tacttaccgc ctatttgaag ttttgcagct gcatcctgat aagtcagatt
    10561 ctgcatgcca cctgtttgaa ttaaatcatt cagaatatta taatcaacat taccttcatt
    10621 aattgagtaa taaaactgac caccggtctg tgtcaaactt ccatttatcg atacagtaat
    10681 atcatgctgc ttcgacccta aagaagttga accgccaata gaattaacac ctcccagaat
    10741 atcaaaattg ccattaatta caatactttc atttccacct ccaacattac ttgcaaaaat
    10801 tgcactgtta tttccactga tttttaaatc accattaata gttaaattta gtgcaggatt
    10861 attccaccac aaagtattac ttatcaaaac aaatctgtcg cttgaatttt gaaaattaat
    10921 atttacgtct gatcctgttg ttattgaaac tgttttgcca tcggtaatat aactatcaaa
    10981 aactttaaat gttcctccgg aaaaatttag gctgtcaata aaaactgaac atgctgcata
    11041 attaccgggg aatggatgaa acacaacttc aaaatttgca ttcgtttgat taaatattcc
    11101 atttgaaata aaattaaccg gcccacgatt ccatgaaaca ttaaacatgc ctccacttaa
    11161 actacaatta ccatctatga caaatattac tgaatctgtt ccatagggat aaaactgcga
    11221 caccaattgt gttgacgatc cactaacact taagtttcct gaaatatgag tatttaactt
    11281 cccacttgta aaagccaatc cgacatttcc gctggtaaca aaaaaatctt ttgaaacatt
    11341 gatttgttgc aatgcactgt ttgtaaaatt attaataaat acaaataagc tgcttgctgt
    11401 ataactaaaa ctaccattaa tattaaaatt tgttgagcca tcagaaccct gtaaataaca
    11461 actctctgtt ccactgattg aaaggttatt ggttgtaaaa tcaagattta ctgtgttata
    11521 atctgccagt cggaaccagt tgccggctgt cagattacaa taagtattac ctgaaacctg
    11581 aataacacca ttaccatcaa cgtatctgaa gaaatcaact ttacctttta caacactcaa
    11641 atcgcctcca atatttactg tagccccaaa agcatgatag cctgctgtac cttcaactgc
    11701 actaaaatca tgactgaggt aaacactacc tgcattgttc cattctaatg tgccatacgt
    11761 acgaaacatc aatgctgtca acgcattact tttactgcta tcggtaaacg atagtgtatt
    11821 taaggtcaaa tttctattag caccttgtgc aaaaatgatt gctcctgttt tataaatgct
    11881 tacatgatta agatttaaaa gtgttgacat acctgtacca tcatccatca caacctgacc
    11941 attagttact gataaattcc cttttatttt tgaaggtgca aacaatccat cctgttgcca
    12001 aactgcatta tagtttaaaa tgagattacc aactgttgaa ctgataccgg tcataaaagg
    12061 cactgctgct gcactccagt tattaacttc aatggtagca ttgtcatcaa tagattctgt
    12121 accattaaaa attgttgttg ctgcatttga attattgttg tgcacatacc atgcatttgc
    12181 ctcaatggtg aagttactat taactatcac aggtaagtta gcaatgagtt ttgctcctgc
    12241 tttaataatt aatgaagtta acgcaggcgt cactacatct acattaatct gatgatagat
    12301 tattacatta tctgtacata atggtatctt gccaccttgc catgttgccg gattgctcca
    12361 tgagccattg gctacagatg ttacggtctg accggtgccg gcacatgaca atgttccgtg
    12421 attaataccc gcaccggctg cattgccgga aatggtggct gtgccaccgc ctggggattt
    12481 gcgaagtata tttccagata cattacttgt tgatgcacgt gcatagatat ttgaaattat
    12541 cagttcgtca gctttgggga ttccactgac agaaatagtt acagtaattg ttgttaaagt
    12601 cacagaaatg gatgctgcag taatatttct tgatgctaaa aaagaaacat ttcctgatcc
    12661 tggttgaaac ataaatccgg caggtgcact aaggattaaa gttttgttgg attgtattga
    12721 aaaatcacca tttgagcctt cagtaataac aatatcgcca atgggaagaa atgacgctgg
    12781 tgtgatattt aagcaattac cacttgttgc tggcgaaacg gttactgcgg cattaacact
    12841 gatgtttatg aaaagcaaca tcaggattaa ataactaatt cgggacataa ctatagtttt
    12901 atcccgtttt ctacgctaaa atttaaaaaa agttccgaaa aattcttgta aaaactactc
    12961 tgaaaattta ttaattaatg tagtttttct cttttcagca gataagccaa caaaacctga
    13021 tgataacctt catggatatc ggcctgaaca aaatcaattt tatactgcgc acatttatga
    13081 ataagcatag atttatattc tttcatagct tcaatatatt tttctctcac catgttactg
    13141 tgcactttta tctcttcacc ggtctcaaca tcaacaaagc ggtaaggtct gttttcaaac
    13201 tgaaaatcaa gctcatgttt tttatcagta acatgaaaca gaatcacttc atgtttgttg
    13261 tgtttcaggt gctgtagcga tttaaacaaa gcttcattgc tatccatgtt ttcaaacata
    13321 tcactgaaaa taacaatcaa tgagcgtttg tgaatactct cggccaactg atgtaatgca
    13381 tctgcagttg cagttttacg gtttaaatca gctttgtttt ccaacaattt ctcgagatgt
    13441 gcaaatacaa gtttatgatg agctgtactg gattttgcct gagtttgtac gtctatctgc
    13501 tctgaaaaaa ctgtaagtcc aattgcatca cgctgttgtt tcatcatgta caaaattgca
    13561 gcagcactat aaacagaaaa tgtaacttta ttttgtaagt cctttttgcc tttcacttcc
    13621 ggatagtgca tagaggcaga gttatcaata acaatatggc agcgcagatt ggtctcttcc
    13681 tcaaatcgtt ttacaaaaag cttatctgtt cttccaaaaa gtttccagtc aatatgtctt
    13741 gttgactcac cggtgttata gagcctgtgt tcagcaaact ctacgctaaa accatgaaaa
    13801 ggacttttat gtaaaccggt aataaatccc tctaccacct gcttggcaag caattccaga
    13861 tgagaaaggg attgaacttc ctgatgattg accggcaaaa tattatttta taaaattaaa
    13921 aactacacta actgtgtatc aattttcttg gcaagaatat tttttggtgc agcacccacc
    13981 tgcttatcca caacctgccc atttttgaaa aataatattg ttggaatgtt tctaacgcca
    14041 aactgcattg taacatttgg gttgctatcc acatcaattt ttccgattac tgctttccct
    14101 tcgtaatctt ttgccaattc ctcaacaatt ggccctacca tgcggcatgg tccacaccac
    14161 tcagcccaaa agtcaaccaa tacaggtttg tctgatttca ttacaacttc ctcaaagttg
    14221 ctgtctgtaa tttctattgc cataattatg ctatgttttt ttatgtgata tttcagaaca
    14281 ataatagtgg tttaaagttt tatgattttt aaagatattg atattttttc attttttata
    14341 gtctcgagcc aattttttgt caaacaatgg ctaaaactta ttgcttttgt aactaaagtt
    14401 actatttaat gaggcttaca cgctcagaaa ttaagttcaa ttccttaaac aattctttat
    14461 tgtattctaa tttcaactga tttgattcta catcaatact tagattttga tcattgtccc
    14521 gaatactgaa tttgactgtt aatttgccct tactattttt agaaatctct ttcaactttt
    14581 ttattttcat ttcattgata gactcagaac gaagcgaaat cattattccc tttgccattt
    14641 tatttgccac ctcatccaga aaatgaactg aaataatttt taattcaaat tcattctcat
    14701 tataaaactt tgtctgtacc cttgccttga taaaaacaaa agcatcgtta tttagcagat
    14761 gtttaaattt ggcatactct tccgaaaaga ctgcaaattc aaaggaagca ttgtaatctt
    14821 caataataaa actaccaaaa ggcttacctg ttttgctgac tctatgattt gcatttgtga
    14881 taataccggc aaaatttaca tcccggttat ttagttgttt aagatcggca agttgttcaa
    14941 cggaaatatt acaaaatgtt ttaatttcct ctttataatc atcaagcgga tgacctgaaa
    15001 gatagattcc gattatttct ttctccttct ttaattgctc cagattacta aagtgctctg
    15061 ctggaataat tttaggctta gaagcatcaa caccacccaa atcaccaaac aatgtgtgtt
    15121 gtgtactgtt ttttgcttcc tgattagcac ttccaaaacg aataattttt tctagttgtg
    15181 tttgattatc ttttgggtca attgcaaaat actgtgctct gtgaaatgat gtatcaaaat
    15241 ccaatgcgcc tgccattgct aaattttcaa aacacttttt atttgcagcc cgcaaattca
    15301 ctctacttac taaatcaaaa acatctgtaa atactccacc ttttttacgc tctgttaaca
    15361 aactttctgc agcggcttcg ccaactcctt ttattgcagc taagccaaat cgaatttcac
    15421 cttttttgtt tacactaaac tgcaattcac tctcattcac atcagggcct aatacaggaa
    15481 tattcattcg tctgcattcg tccatgaaga aagtgatttt atctaagttg ttcaggttgt
    15541 tggtgagaac agctgccata tattcacttg gataatttgc tttgaggtag gctgtctgaa
    15601 aagcaacaaa ggcataacaa gtagagtgtg atttattaaa tgcataactg gcaaaagctt
    15661 cccagtctga ccatattttt tcgagtactt tttcatcatg gccattgcct tttgcaccat
    15721 ccataaattt attcttcatt ttattgagca cttcaatctg ctttttaccc atggctttac
    15781 gaagtgtgtc tgcatcgcct tttgtaaaat tgcctaactt ttgtgaaagc aacatcacct
    15841 gctcctgata aacagtaata ccataagttt cctgcaggtg ttcttgcatt tccggaagat
    15901 cgtaggttac tttttctttt ccgtgcttac gtgcaatata attagggata tactccatag
    15961 gtcccggacg atatagtgca ttcatggcaa tgagatcttc aaacatatcg ggcttgagct
    16021 ctttcagata tttctgcatg cctacactct caaactggaa agtaccattg gtttcacctc
    16081 gctgataaag tgcaagagta cgactgtcat ctaaaggtat ttcatcaaga ttaattttta
    16141 caccatgatt tttttcaatc aatctgatgg catcacgcaa aatggttaag gttttaaggc
    16201 ctaagaagtc catttttatt actccggcat cttcaatgat ttttccttcg aactgcgtaa
    16261 tcaataggtc tgtttctttt gaagtagaga caggaataat atccgtgagg tctgttggcg
    16321 caatgataat tcctgctgca tgaatacctg tattgcgtac actgccttcg agcaccattg
    16381 cttctttcaa tacagatgat tgtaatgaag ttccttcttc aagaattgag cgcaatgatt
    16441 tcactttctc taattcatca gcacttaaat tttcttttgt cttcaaactt ttttcgccat
    16501 caattggtgc tgtcagtaaa cgacccaatg aaatacccgg acgatcaggc actagcttgg
    16561 ctaaggcatt agaatcaggc aatggcaagt caagcacacg tgccacatct ttaatactca
    16621 ttttagcagc cattgtaccg taagtaataa tttgtgctac ctgatttttt ccatacttat
    16681 ccactacata atcaatcacc ttctgacggc cttcatcatc aaaatcagta tcaatatcag
    16741 gcattgactt tctgtcagga ttcaaaaaac gctcaaacag taagttgtat tttattgggt
    16801 ctatgttagt gatgccaata caataggcaa ctgcagagcc tgctgcactt cctctgccgg
    16861 ggccaaccaa gacaccaata ttttttccgt gattaataaa atctgcaaca attaaaaagt
    16921 aaccggcaaa tcccattgtt tttactgtaa acaactcgaa gtttatgcgc tcttcaatat
    16981 caggagttat ttcactataa cgttttcgtg ctccttccca tgtaagatgt ttcagataat
    17041 gatcctgatt ttcgaaacct tgtggaatct gatagtgtgg taaaagaata tcctgcttca
    17101 gttttaatgg ctcaatttta tctactattt cattggtgtt atcaatagca tcaggtctgt
    17161 cactgaagag ttgctgcatt tctgatgtgt ctttaaaata aaactcatcg ttataaaaag
    17221 caaatctttt atcacgtgca gatgtatctt catcgccatc tttaaacttc tctgtgcttt
    17281 gtttctcacc ggtattgatg caaagcaaaa tgtcgtgtgc attatagtct tcacgattta
    17341 cgtaatgtga atcgtttgat gcaatgattt ttacattgtg ttttaatgca aatccaatga
    17401 ggacttcatt tactgtattc tgctccggaa tatcatgacg ttgcagctct acataataat
    17461 cttctccaaa aatatcgagc caccataaaa attctctttc tccttcggct tctcctttgc
    17521 gcaaaattgt tttgggtacg cttgcaccaa gacaacatgt tgtggcaata agaccttcgt
    17581 gatatttgag tacaagttct ttatcaattc gcggatactt gccatacaaa ccttccatat
    17641 atcccaacga ggttaacttg acaagatttt tataacctat attatttttt gcaagaaata
    17701 actgatggta acgtacatct ttatcgtctt tggtaaaact tcgcttgtga cgattttcaa
    17761 ccaaataaaa ttcacaacct acaataggtt taattacatt ggggttttcc ttggtattgt
    17821 actttgctgc ttcggcaaca aaattaaata cgccaaacat gttaccatga tcggtaattg
    17881 ccaaagcagg catgttgtct tttaccgctt ttttataaag tgacttaata tctgctgcac
    17941 catcgagcaa agaaaactgt gtatgattat gtaaatgaga gaaacgaggc atgaaaaaat
    18001 aagtatgagt ttttgaatcc aaaaaccgat atggtttttg ttgcttgtaa aattaataaa
    18061 acgattcaaa atttctttac ttgttaataa caaaaaccca tgtgactttc agagatgtgc
    18121 atgacctaaa atgaaaaagc aaccgatgtt ggcacatacc ggttgctttt atttgtggtt
    18181 cacttttgtt ttagaccata agcaaatgaa gattattgtt ataccctaaa catttcaatt
    18241 gctatgccat taatattgca ctgtaagaaa gatgtaagaa gatgtgaaat tcaattatgc
    18301 aggtttgctt tctttgatca attcagaata aaattttctg cttttcaggt cgtaacaatt
    18361 accttttgtg agtacatgaa ctatcagatt ttcaatgctg ataggaacgc cttcctgtaa
    18421 atcggcaata ttgttgtggc gaatgtggtg tccgtcaatt aatataacca atcccgaacc
    18481 gataatctcg agtaaataac cttcggacaa caaaacacct gtatcttctc caagtccgat
    18541 accaatacaa cttggattgc ttgcaactgc ctgtgcaagt cggccaaagc gaccgcgctt
    18601 gtcgaagtgc gaatcaatta tgacatcgcc catcagtgca agtcctgtag taattttaac
    18661 ctctccttta attaaagcac cggaactgct accctgataa atcatcgtat tgctcattgc
    18721 catagcaccg gcactggtac ctgcaattac aaaattttca tgttgatatc tgtgctgtaa
    18781 aatttccaga atttctgtac ctccaaaaat agtactcagg cgtaattgat ttccaccggt
    18841 catcattatc ccatcacact tttttatcct ttcaatgtat tcgggctttg ttgcatcctc
    18901 tctgttacgg atatccatca catttacatt ggtacatcct aacttaccaa atgcatcaat
    18961 ataatttcca ccaacctcaa caggaatact gcttgcactg gtgatgactt cgatttttgc
    19021 atttttaccg cccatctcat gacagattcg gctaagaatt tgtaatgcaa aaaagtttaa
    19081 gttatttttc tgttcaaaat taggttcgga ctctgtgccc ttatcttcat tgccaccaat
    19141 agcaataagc tttccttttg gtttattcat ttattgattt gagttatccc ccgccccaaa
    19201 aaatggtatt taaaaattaa aagtcaaaag tacatattta tcactaaaaa gtaaagcggt
    19261 gttgacactt aaaaattatc tttttaacaa ttttttaccc aaaaaaccta tttgtatttt
    19321 cttggaaagt actttttatt cataatatgc taccccggta attcttgcca attctatacg
    19381 tgccagacaa agttgatatt caagattcaa cttagacaat tcggcacgtt gtctgtttgc
    19441 caatgcattt aatacatcgg tataagtcac caatccatta cgatagcgcg attgtgccaa
    19501 ttctacagct gaacgcgact gtaatacttg tctgttagag tttacaatct gtgcttcaag
    19561 cgttgaaata tcactcctga cattacttat atcttttctg aagctgtggc taacattctc
    19621 ttgtgattgc tgcagttgtt tcaaataaag ttttgaaagt tttatactat ttcgagcttt
    19681 attaccgtaa aaaatggggg ctgaaatact taatcctgcc tgtgtaccaa aaactaattt
    19741 ttcaatatca ggttgatagc catttctata acccaatcct gcattaacac ttacaacagg
    19801 aaaaagattt gaccggttca tcttaatatc aaactgctgt gctttaagct tactgctaat
    19861 tattttatat tcaaaacttg attgaacctg attggtggta tcactttcaa ggtaccattg
    19921 cgggaaatca aatttttcag gcataacaat ggacgtttca ttggttgtgt attgcaaaag
    19981 tgcaatctgt tttgacagta aattctgaag tgacaatttt ctgttttctt cttcgctaat
    20041 ggcactaccg gtggttattg aatcaaggat taaagcatcg ccatttctca acctacttat
    20101 aataacattc tgataagaat gcatggcaag taacaccgta tcttcaatag ctacagcttt
    20161 tgaaaaataa gccatactgt aatagactga agccacttgt gcagcaagtt gtgccttgtt
    20221 aagttctata ttaagtttac tttgttccaa ctcctcatta gattttctta caaaaaacca
    20281 ggcgcgtccg aaatctatta ccggataagt aaatcctgct gttgctgtgt aattattgta
    20341 tggttgaaac tgcattgcct taataacacc gggcgcaaca ggaaaggaaa cactacttac
    20401 tggatcaata taattatatc ctgcattgag cataatattt ggataataag cagactttgc
    20461 aatttccaat ttttctttac ctgcctgtag tccaagctct gtttctttca gcttaggaaa
    20521 atatgcaatt gatttttgaa tcaatgtttt caaatcgctg ttgatgtttt gttgagctgt
    20581 tacaattgtt ggcatccaac ctgccagaac tattaaaatt attttctttg acaacatatc
    20641 attttttaat gagcttcttc tgcaactttt gcaagtgtag ctgccgattg ttttttagta
    20701 tctaaaagga atataaatgg catggcacaa atcaggaaaa cactaataag caaaaagcca
    20761 tctaagtatg catgaagata actttgcttt gttaatgtgc cttcaagaat ttgatagccc
    20821 gcctgtgttg cctgttgcgg agagaagcct ttactcattg taaaggctgc aacctgcttt
    20881 agtctttctg tagtttccgg atcattagct gttaaattag ctactaaatc agttctgtgt
    20941 atagctacac gttgtgcaac ataggtattc attatagcaa tcccaaacga tccacccaat
    21001 tgccgcaaca tgttagttag tgaaattgca taaggcattt tctgaggaga aattccaata
    21061 acagcctgat tgattaaagg caccatgaga aatgacgtac caactccacg cataatttga
    21121 ggaactataa accattctcg tgatgcatca aggttggcta atgacgaaac atatccatgc
    21181 aaaataaaaa ctataaatcc tgtcatcaca aaataaatgg gtctgatgcc gctgcttaac
    21241 cctttaccga taaatggcat catacaaatt gctgctattg ctcccggaac aataccaagt
    21301 ccggcttcca tgggtgtata tcctaacaca cgttgcacta gtacaggaaa aataaacact
    21361 gaaccaaaca aaccaaagcc gcagacaaaa gtgaggacgt tactggcaaa caaattcctg
    21421 tttttaatta ttcttaaatc tattgcagga tgaggatgac gtagttcatg ccaaataaaa
    21481 aatccaattc caaaaacaga aagtatgctc aggtaaataa tgtctctgct gttaaaccag
    21541 tcttctgttt gtccgcgctc aagaacatat tgcagagaac taacaccaat agctaacaac
    21601 aaaattcctg tatagtctat atgaatttct ttcctgttga tgttgatttc ttcaggtttg
    21661 cggtcaatat atagccaggt gagcagcaca gcgacaatgc caaaaggaat atttataaaa
    21721 aatattaatg gccaacttga atgttccata atatagcctc caagtgttgg accaattgtt
    21781 ggaccaagta caatacccat tccaaacata gcagatgcca tagcacgctt ttccggttgg
    21841 taagcatcaa acagaatact ttgtgatgtt gataacaatg caccaccacc aataccctga
    21901 acaaaacgcc atgcaattaa ttcaataaga gaatcagact cgccacagag gtaggaggca
    21961 gcagtaaaaa ttacagtgct cgctaaataa taattctttc ttccgaaata ctttgccaaa
    22021 aaaccggtta gtggaataat gatgacgttt gcaattgcat aagcagtaat caaccaggaa
    22081 gtatcttcaa ttgtagcacc caggcttcca cttatgtctg tcaagcctac attaacaata
    22141 gaggtgtcaa tcaactccat tatggcagct gaaatagtag tcagaacaat gactattctt
    22201 ttaaaacctg atgggtatga agcaggctct gtcatttttt cacttcaaca gtaacactca
    22261 ttccggcacg aagcatgttt ttatactcac taaagttctc aatggcaatc tttacaggta
    22321 ctcgttgtgt tactttcaca aaatttcctg ttgcattgtc aggaggtaac aaggcaaagc
    22381 gagatccggt ggcttcactc aatgatgcta tccgacccgt tattttttga tcaggaaatc
    22441 catcaacaat aaggttggct tcaccacctt ctttcaaact tttaagttgt gtctctttaa
    22501 aattggcgac cacccaaaat ttttcactat taactatcgt aaatagcgtt tgccctgctt
    22561 gtaaaaactg acctatattg atattgcatt ttccaatttt tccggttgca ggtgctgaaa
    22621 tactacagta agaaagtctg agttttgcct gatccaacat cgcctttctt gtttctttaa
    22681 gtgcttcggc acgcttcacc attgatactg cggtttcttg ttgtgccatt gccactttaa
    22741 tctgatccga agtcacatct aatgacttta gcatggcctc catattaatt ctgctgtcat
    22801 ctatttgttt ttgtgtgatg cttccatcat taaacaatgc tttgtcacga ttataatcag
    22861 ctatggcctt atcggtttta attttttgca attctttgtt agtttgtgct acctgcaagg
    22921 cagcttctgc atttttcttg cctgcatttg cattcaataa ttcagcttct gcactttgta
    22981 catctgcttc agcttgttgc tctgccaaaa taaattctct tgaatcaata cttactaatg
    23041 gcattccttc tttaacctga ctaaagtctg ttatactcag actgtcaaca taaccactga
    23101 tgcgactgat aacaggaata ccggcagctt caatttgtgc attgtccgta atctcatacc
    23161 tgaggttatg aatcagattt ttaatgccta aaaaagctgc tattgcaatg actacaatgg
    23221 caatgacaaa tagcgggttg aatttctttt tatcctgttc cggagattcc atattttctt
    23281 gaattgggcg tcaaaagtaa acattgttga ctatttagtc aagtatgttg actttttttt
    23341 atttttgcac catggcagga aagaaaaaag atattaccca atatgcaagg ttcaatatcg
    23401 gacgcttaat ttctatatat aagcgtgcat ttgatgagtg ggctatggca ggtcttgcag
    23461 aacgaggtta tcctcagttt aaaatttcat attttgcagt gttgatgaat atcaatgaaa
    23521 atggtaagac caacaaagaa attgctgctg cagccaaagt cagtaagcag gccatgagta
    23581 gaattgtaaa agaattggta agcatgaaac taattcaggt tacaacacat cctgacgata
    23641 accgtagtgc cctacttaaa cttactacta aaggtcgtac tatggttaat gaaactaaag
    23701 aggtaatgtt acaattagag tcacattatg agaaggcaat cggaaaaaaa gaatttttaa
    23761 attacaaaga aacaacagca aaactcacca accttcattg gcccgaattt tacccacacc
    23821 attaatctga attaccgata aagtgcaatt tgtaggttat ctacttacgt aataagtcat
    23881 ataattcatc gttctcggta aaacggtatt tatatgatca ggcaagctat ttgtcttatt
    23941 gcattgataa cttatgcaac aagtacttcg gcacaggagt taattgactg gcaggagaaa
    24001 cgtttaacat gggatgattt tgaaaaaaga aatctacctc atagcagaac tggtgcaatt
    24061 acatcaagtg ggatctattt cagttatata gagcataacg atggattaga catcatagta
    24121 aaagctcaaa tggataaaag cagatcgtgg gtcaacacac actcattgcg cgatgaagtg
    24181 ttgtcgcatg aacaagtgca tttcgatatt tctgaaattc atgcgcgctt attacgtcag
    24241 tattttgcat taaacaatag gaaatctgcc ggtcgcaatc ctgaaagtat ttataaaaaa
    24301 tacatcaaca gacttaggcg tatgcaggat gattatgaca gacaaaccaa tcatggcaat
    24361 gacctatata tgcaaatgat gtgggaggaa aaaataaaag ctatgctgat agatttagaa
    24421 caatattccg gtcaggaaat ggcatggaga caataacaga ataagttctg ataaaagaaa
    24481 accccattca ttactgaaca gagtttcctt aattcttgct ttgttactta tctaagatcc
    24541 acaagccaaa cagtcatcct tattatcaat gctacaactg atttcggcca tagcttcttc
    24601 cttactaaca ctttgtgttg tagccacgat attatgattg ctgtgccctt caaccgtaaa
    24661 tttaattgca tcggtagctg cttttgttcg cagatagtac atgccggttt ttaatccctt
    24721 tttccaggca taaaaatgca tactgcttag tttggcaaaa tttgggtctt gaataaaaag
    24781 atttaacgac tggctttgat caataaatgc accacggtca gcagccatgt caataactgc
    24841 gcgttgtttt atttcccaaa ctgttttata cacttctttt atctgctcgg gaatttcagc
    24901 aatgtcttgt actgaaccat tggctgcaac aattttattt tttatttcgc tattccacaa
    24961 attcaacttt accaaatcac gcaacaaatg tttgttcact acaataaact ctccgctcaa
    25021 caccctacgc gtgtaaatgt tgctggtgta gggttcaaaa cattcgttgt tacccaaaat
    25081 ttgtgatgta cttgccgttg gcataggtgc taaaagtaaa ctattataaa caccatgctt
    25141 cagaatttct tcgcgcagca catcccattc ccaacgatcg gttggtttta cattccacat
    25201 atcaaattga aattcacctt tagaaattgg tgaaccttca taactgctgt aaggaccatt
    25261 tacctttgca agatcttttg atgctgttaa tgctgcatag taaattgttt caaaaatttc
    25321 ccgattcagc aaacgtgctt cttcgctctc aaaaggataa cccaataaaa taaatgcatc
    25381 tgcaagtccc tgcacaccta aaccaatagg tctatgacga agattgctgt tgcgcgcttc
    25441 cggaacagga taataattta tgtcaataat cttattcaga ttttttgtag caacataggt
    25501 tatgtcaaac aacttctgat ggtcaaatgc accattgttt acaaatcgtg gcaatgcaat
    25561 tgaggccaga ttacaaacgg ctacttcatc aggagcagta tattcaataa tttctgtgca
    25621 taggttggag ctctttattg tacccagatt tttctgattg cttttctcgt tgcatgcatc
    25681 tttatacaac atgtatggcg taccggtctc aacttgcgac tcaagaatag cactccacag
    25741 ttcgcgtgcc tttattgtct tacgctgacg gccttcattt tcatacttcg tgtaaagcat
    25801 ttcaaactct gccccatagg tgtcggcaag acccggacat tcatttggac aaaacagact
    25861 ccactcgcct tctgcctcca ctcgtttcat aaacaaatca ggaatccaca tggcgtaaaa
    25921 caaatcacgt gcacgtagtt cttccttacc atgatttttt ttcaggtcaa gaaattcaaa
    25981 aatatctgca tgccatggtt cgagataaat tgcaaacgag ccttttcttt ttccaccacc
    26041 ctgatctaca tagcgggcag tgtcattaaa aacacgcagc attggcacaa taccattgga
    26101 tgttccgtta gtacctctga tgtaagagcc tgtagcacga acattgtgta tgctcaggcc
    26161 tataccaccg gcactttgcg aaatgagtgc agtttgttta agcgtatcat aaatgccatc
    26221 aatactgtcg cctttcattg taagtaaaaa gcaacttgaa agctgtggct ttggtgtgcc
    26281 agcattaaat aaagttggtg ttgcatgtgt aaaccaacgc tcactcatca ggttataggt
    26341 ttcaattacc ccttctatat cttctttatg aataccgaca ctcacacgca taagcatgtg
    26401 ttgcggccgt tcaacaacct ttccatttag ttttagcaga taagatttct ccaatgtttt
    26461 gaaaccaaaa aaatcatacc caaaatcacg atcataaata attgtactgt caagaagttc
    26521 tgcattcttt tcaatcactg cccaaacatc atcagcaagc aatggtgcaa gttttcctgt
    26581 cttggggtca atgtaggtgt ataaatcttt catagtttcg ctgaaagatt tttttgtgtt
    26641 tttatgcagg ttagaaatgg caatacgcga tgccagcaaa gcatagtcag gatgtttcac
    26701 tgtcatagtt gcagcaactt ctgcagcaag attgtcaagt tcagaagttg taacaccatc
    26761 ataaacacct tcaataacac gttgtgctac attgagtgaa ctaacatgat gtggatctag
    26821 tccataacac agtttttgaa ttcttgctgt gattttgtca aactttacgg actctttttt
    26881 gccgtctctt ttaattacgt acatgtgatt tttcttgtta ggtggttact ttaaattgtt
    26941 ggttgattat tgtaatttca ggtagcataa attaacagat aaaaaagccg gcagaaaaaa
    27001 atattttcta ccggctttca acagttggtt gttaaaagtc ttcgtcaaac ctgattttct
    27061 gagattcctg tgactgagcc actccggctt ttttatattc gcctacacgt ttctcaaaaa
    27121 aattcgtttt accctgtaag gaaatcattt ccataaaatc gaaaggattg gtggcattat
    27181 aaatttttga atagcctaaa gaaaccaata acctgtcagc cacaaattct atatactgac
    27241 acataagtct ggcgttcata ccaatcagag aaacaggtaa tgcatcagta acaaactcat
    27301 gttcgcagtt aacagcatct tttataattg tatgcacttg ctcttctgtc attttgtttt
    27361 gaagcatact atataataag catgcaaaat cgcaatgcag accttcatca cgcgaaatca
    27421 actcattgga gaatgtaagt ccgggcatta atcctctttt ctttaaccag aaaatagaac
    27481 aaaaactacc agagaagaaa atgccttcta ctgctgcaaa ggccactaac ctctcagcaa
    27541 agcttccatt ctgaatccaa cgtaatgccc agtcggcttt tttctttacg caaggaactg
    27601 tttctatggc attaaataaa tattgcttct ctttagggtc ttttatataa gcgtcaatta
    27661 ataaggaata tgtttcggag tgaatatttt ccatcattat ctgaaaacca tagaaacagc
    27721 gtgcttcagg aatctgaact tcactcataa aatttacagc aaggttttcg ttcactatac
    27781 catcacttgc tgcaaaaaat gcaagtacat gttttataaa atgacgttca tcgttattga
    27841 gcttattctc ccagtcaacc aaatcaggat gcaggtcaat ttcttctgct gtccagaaac
    27901 tttgttcatg ctttttgtac atctcccaaa ccttaggata gttgattggc aacagcacaa
    27961 agcgttcttt gttttctctt aaaagtaatt cgtcttgtat catatttttt tattggtaaa
    28021 aaagttcctc cacagattat cttttcataa tctgttattt gtttattata gtaaatgaat
    28081 aatttttccg aaatctaagg gtggtaatac cgcttgcaat tccgtttaca aatcaagtta
    28141 aaaataacat tgaactatgg ttgttttttt aaacaatcaa caacactttt gaacaaaaat
    28201 cacgttgaaa atttttttgt cttattttat ttgcagaatt aacttttttg atttttactc
    28261 taaaagccat ttgataatat tatatctgag ctaaccaaat attttttgaa gtaaaagata
    28321 aaagcgacaa atcaaggtat aatgcaatca atatcagtaa agttgaacta atcaacagtt
    28381 gttcatttct gcaacgtagt tattttacta cttcgtgctc gaaattaaaa ttgcctgcag
    28441 ttaccgaata agccatcaat ctgccatcat cagaataagt tgtctcaata tttactcgtt
    28501 taaaagtaag tcggttgact atcttaccaa aaactgcaag tgtttttaac ttgcctttct
    28561 caacgggtag tcctgcaata tcttctgtaa ttgagttaag cttttgaagt gcaatgtcct
    28621 ttataatatc ttgctttctt tcggtttgga ggtctgtgtt tttcatcacc attgcataag
    28681 agttttcaat attagcaaca ggcactgg
//
LOCUS       DAOQNP010000029        28582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_520_length_28582_cov_9.515897, whole genome shotgun sequence.
ACCESSION   DAOQNP010000029 DAOQNP010000000
VERSION     DAOQNP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28582
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_520_length_28582_cov_9.515897"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKY09_05750"
     CDS             complement(<1..330)
                     /locus_tag="PKY09_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12395.1"
                     /translation="MKKYLLIILLSAYAARVLSQGSVTPSELLTIAFEDATGARDTVY
                     IGHYMDSCYLYINPPPSCPKIVSPNIDTAFGEVDLGSLVQPGVFTARIENKMSDGFAY
                     DWYTKYDI"
     gene            complement(596..2236)
                     /locus_tag="PKY09_05755"
     CDS             complement(596..2236)
                     /locus_tag="PKY09_05755"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNB12396.1"
                     /translation="MRNFFLFLIVLVLQFSIATSQTLQWAKQFGGIQVDIATSNAIDQ
                     AGNIYVTGFTEASSPDCFIYKIDSNGVILWNKLIGGPGVDGSYEIVCDNNSNIYISGY
                     FGDSVDFDPGPGVTLLKSQSTSSAFLLKLDSTGNFIWVKVVSGSEFSYDRGTSCVVSD
                     GNVFWAGIFSDTLHFIDITNDSLMGHSKGISDVFIAKLDGGGNLNWIKTFGGGDEDYV
                     NSIKLNSSGDILLVGEFKGLCDFNPDTVINYDLLSYGYSDIYICELDSGGHFAWAKQL
                     GGANYSIGSNLTIDNLDNIYGIGYFIDTLDADPSANVFNLISNGLTDVCLFKLDNGGN
                     LIWAKSWGSTGSDEGMGIVIDSVNNIYTTGAFNDTVDFDPGTGIYNLSSIGGINTFIS
                     KLESTGSLIWANQIKGSNNIGLSIKLDAHEKIYVAGEFGGTCDFDPSNNLYNLTSTGT
                     DDAYLFKWSQPLTGLHDTYTNSSVKVFPNPFTETVQINVIEKATVTISSITGQVILRT
                     ILPAGINTITLANQQSGTYLLNVLTDKSSSNAIIVKSD"
     gene            complement(2254..2664)
                     /locus_tag="PKY09_05760"
     CDS             complement(2254..2664)
                     /locus_tag="PKY09_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12397.1"
                     /translation="MKRLSKPISAIATVLLLNQNAMAQECEFVDPSPPSALQGATYES
                     KNGFSISPYGNYRVLNLMINIIYDATPNDPLAGAPSNYWDPGTSNTVNSNPPTNLTSF
                     MDADFATVPPQGIFTRKYYELSFGALTVKKILYL"
     gene            complement(2639..3460)
                     /locus_tag="PKY09_05765"
     CDS             complement(2639..3460)
                     /locus_tag="PKY09_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12398.1"
                     /translation="MKRLSKPISAIATVLFCVFFSIQAFAQPQWKFHIAFEDATGAKD
                     TIWCIWDTTATFGVDIQFNEQAVSLNYNQFNVFIGNTNGDTTKTQALPHPHSSNDVIV
                     YAINYQYPIMVSWDSSLFHVPGLPLPVGYINRAFINNDYFFAVNNDPPSHVFNMLWDN
                     QVLAPSFSWGAASQFPMGFYIMRDETIGIGENFIENEDISIYSNSTTGVLIKSEKSIT
                     LIEVFDLYGNIIKKYSNIYNKQYSFQINDLTNGIYIIQITDNSKNQYHEKIIKTN"
     gene            complement(3435..4253)
                     /locus_tag="PKY09_05770"
     CDS             complement(3435..4253)
                     /locus_tag="PKY09_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12399.1"
                     /translation="MKKLLLIKFAFGLFTASMLNAQPQWKFHIAFEDVTGAKDTIWLV
                     YDTSATGGFPIQVDSALGEGAYPFDYSKFNVWLYNDIGDSTKTYALPYLYYPEHSANI
                     NAFNYQYPITVSWDTSLFHASYLPVQQGIFNVSRMDNDYFFAINNCIGCHYFDMMIDD
                     HAFAPAFAWGSASQFPLDINLLYDPTIGLNTISKEQYKIFPNPVTDNLRISIKNHVDN
                     IQLSDLTGTVFKEYRQINSDDFIISMKDIPNGMYIIQITDNSKNQYHEKIIKTN"
     gene            complement(4258..4476)
                     /locus_tag="PKY09_05775"
     CDS             complement(4258..4476)
                     /locus_tag="PKY09_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12400.1"
                     /translation="MRKIKIIIILMLTGKLLFAQCEYIEDSTALRVNGTVSAPACSGN
                     FTYCSKNGYSISPYGNYRVLNFIFIPKL"
     gene            complement(4516..6150)
                     /locus_tag="PKY09_05780"
     CDS             complement(4516..6150)
                     /locus_tag="PKY09_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNB12401.1"
                     /translation="MRNFFIFLIVLFLKFSIASSQTLQWAKQFGGAQPDAALSNAIDF
                     TGNIYITGYTDTEIFINKVDSNGNTLWSKIIGGPGVDESFHITTDTICNVYICGSFSD
                     SVDFDPGPDTCMLISHGYDAFLLKLDSAGNFIWIRQLGGDKSIDDDHSRSCIVKDNIV
                     YWCGIFTDTLLHLAPNISLPIAVSHGYEDVFVLKMDSSGNILWSKTIGGIQREEIAAI
                     AIDNTNNLLLTGMFNGLCDFNPDTTVNFNLTSFGQFDVYVCKFDSAGNFIWAKQFGGA
                     QHGNGTDILSDQLGNVIISGYFSGIMDVDPGSTVFNLSSHGNRDVFLVKLDANGQLIW
                     AKNLGGTGTDMGMKLSLDSSANIYTTGRFADTVDFDPGVGVYNLFGNSSGDTFVSKID
                     SAGNFVWANQLTGTTDNVGTSIALDKNQNIYVAGGFHGYCDFDPSNNSFNLISSGIDD
                     AYIFKWSQPITGLHDNYTNTLIKVFPNPFAENVQINVIEKATVTISTITGQVILRTIL
                     AAGINTITLASQQSGTYLLNVLTDKSSSNAVIVKSD"
     gene            6602..6778
                     /locus_tag="PKY09_05785"
     CDS             6602..6778
                     /locus_tag="PKY09_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12402.1"
                     /translation="MDLLISILLYLGVYATPADLQNKEFVAKNSEKINQAETLSKDPN
                     FKCDGIVIVGSGNL"
     gene            complement(6821..8323)
                     /locus_tag="PKY09_05790"
     CDS             complement(6821..8323)
                     /locus_tag="PKY09_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12403.1"
                     /translation="MEFSEIKLLINFFAKNRHDFLGVPDVYFADKNYPELLWFYKEIS
                     KGSINNDQEAAEKLLQSTTTNPAYQQLKAELEDRLVNLVFGLDPEKLMNSMLGRSSFR
                     AYIYFGAAMILRQQNASAFFSDHFFKKAADYATFTNDGMIGCLTDLMLADRMAIKNKP
                     DDFYHYRNRFKKSIKQFELEAELILMQRELDIFQVASNISQYKNTAKAKRYYLRAQKI
                     HQQLKTFNSNFYYGNIAFIYGYFSSNFELVEEALNEREAFYLNHPEYYSDFQKSFIAL
                     SRMLAYIHLRQYDRGKEEARIFLGKLSANSSDWLHALEHYFILCMHSGNFEQALNIYY
                     EATRSEYFINSNQEVKERWHYFEPYLNFILPGHFPKESINLLGFLDEISYYTTHKEDN
                     NIIIMIGQIIVMIEMGDYDKLYDRTSFLESYIKKYVDRRIYPRTFIFLNMLLTLFKNN
                     FQKTKTQKQTAISLEKLKPVTGNKFFNTEGLEVIPYEKLWAAILEKLKRH"
     gene            complement(8434..9153)
                     /gene="rnc"
                     /locus_tag="PKY09_05795"
     CDS             complement(8434..9153)
                     /gene="rnc"
                     /locus_tag="PKY09_05795"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNB12404.1"
                     /translation="MRFYSFFPINILFSADRKTYFSLHNILGFYPIKLSLYKLAFSHR
                     SQTKEITNGIKHSNERLEYLGDAVLGAVVAEMLFKKFPFREEGFLTEMRSKIVSRENL
                     KHLAFKLGINHFIKKTHGPDSFRNIYGDALEALIGAIYIDRGYDKARQFILERMINHH
                     VDLESLEATEINFKSRILNWSQRTKNTVAFETIEDEKSNDKLIRVRLMINGEEVATGM
                     DFVKKKAEQIAAEKACSKLEI"
     gene            complement(9160..10413)
                     /gene="fabF"
                     /locus_tag="PKY09_05800"
     CDS             complement(9160..10413)
                     /gene="fabF"
                     /locus_tag="PKY09_05800"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNB12405.1"
                     /translation="MELKRVVVTGLGALTPLGNSVSEYWDGLLNGVSGACQITKFDAS
                     KFKTHFACEVKNFDPLVYLEKKEARKLDPFSQYAIAVSDQGVKDAGLDTAKINHDRVG
                     VIWGSGIGGLRTFLDEVIAYAKGDGTPRFNPFFIPKMIADIASGHISMRFGYRGPNYT
                     TVSACASSTNALIDAFTYLRLGKADIIISGGSEASVNEAGIGGFNAMHALSERNDSPQ
                     TASRPFDLDRDGFVLGEGAGCIVLEELGHAQARGAKIYAEMVGGGMSADAYHLTATHP
                     EGLGAFNVMQSSLDDAGMTSADIDYINVHGTSTPVGDPSEIKAILKLFGDDAFRLNIS
                     STKSMTGHLLGAAGAIEAIASILAVQNNIVPPTINHFTDDPAFDPRLNFTFHKPQQRN
                     IRAALSNTFGFGGHNASVIFKKFTP"
     gene            complement(10425..10661)
                     /locus_tag="PKY09_05805"
     CDS             complement(10425..10661)
                     /locus_tag="PKY09_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNB12406.1"
                     /translation="MSDIASRVKAIIIDKLGVDEGEVTNEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKISTVGQAVDYITANVK"
     gene            complement(10819..11814)
                     /gene="trpS"
                     /locus_tag="PKY09_05810"
     CDS             complement(10819..11814)
                     /gene="trpS"
                     /locus_tag="PKY09_05810"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNB12407.1"
                     /translation="MDTVVSGIRSTGNLHLGNYFGAIKNFTAMQHEYRCFFFIADYHS
                     LTTHPTPENLQQNVKAALVEYLACGLDPEVCTMYIQSDLPETAELYLLLNMNAYVGEL
                     ERVTSFKEKIRKHPDNINAGLLTYPVLMAADILIHKATKVPVGKDQEQHLEMSRTFGN
                     RFNRLYKTEYFGEPMAFNFGNTLVKVPGLDGAGKMGKSESEGNALFLKDDAATIRKKI
                     MRAVTDSGPTEPNSAKPEAIENLFSLMKLVSSSDTISHFETSYNNCNIRYGDLKKQLA
                     EDMVTFMAPITQKILDIEKNHDYVKKVVLLGREKAKASAQKTIEEVRQIIGIRRF"
     gene            11909..13180
                     /locus_tag="PKY09_05815"
     CDS             11909..13180
                     /locus_tag="PKY09_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNB12408.1"
                     /translation="MIPLAERMRPKSLNEYIGQEHLTQTGAVLAKAIEKNTIPSMILW
                     GPPGVGKTTLAFILANSLSRQFYHLSAINSGVKEVRAVIETARQHFESVQQPSILFID
                     EIHRFSKSQQDSLLGAVEKGIITLIGATTENPSFEVISPLLSRCQVYILKALTAEHLR
                     TILHAALTNDSVLKEQKVTIKEDDALLRFSGGDARKLLNLLELVIQVNQQDEICIDNK
                     MVEQTIHQKMALYDKTGEQHYDLISAFIKSIRGSDPNAAVYWLARMIHGGEDPLFIAR
                     RMLILASEDIGNANPNAMLIANQCFDAAHKIGFPECRIILSQAAIYLACSPKSNSSYM
                     AIEEALELVAKTGDLPVPLHLRNAPTNLMKEIGYGKDYAYAHNYENNFVKLNYLPNQL
                     KGKKIYFPGKNVREAEFQQFLKTRWENEYDY"
     gene            13274..14608
                     /gene="creD"
                     /locus_tag="PKY09_05820"
     CDS             13274..14608
                     /gene="creD"
                     /locus_tag="PKY09_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNB12409.1"
                     /translation="MENTSHSFFDRLNQWIKNSVMVKLISMGILILLLLIPSEMVQSL
                     INERQQTHDSALDEISSKWGFEQHLSGPVINVPYQSFFVDADGKRKSQIQYAHFLPEE
                     LKIAGTLEPEMRHRGIYEAVVYKSDVSLKGYFRFPDFAEWNIAPSDILWKETTLSLGI
                     SDLRGIKQRLTINLNGNPIEFNPGVENNDVHESGVSIRLPIADSLNKPDFKFDMSFKL
                     NGSKEMYFIPLGKETTVSIKSDWTNPSFDGAFLPDTHHITDKGFDAEWKIFHLNRNYG
                     QKFRGAPTGLQESSFGVKLLIPVDNYQKTYRSAKYAILLITLSFTLFFFIEVLNRKRI
                     HPFQYILVGVALCLFYTLLLSVSEYISFNLSYLLSATAIILLISLYAKTIFNNLRLAL
                     LFSLVLIILYAFVFSIIQLQDYALLLGSLGLFIVLALVMYLSRKINWYGTEK"
     gene            14592..14969
                     /locus_tag="PKY09_05825"
     CDS             14592..14969
                     /locus_tag="PKY09_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNB12410.1"
                     /translation="MEQKNKGDHYQLGREGENMAVEYLVANGYQIVERNWRFGKLEID
                     IIASKGNLLCIVEVKTRTGDYFGEPEEGVTKAKERFLATAADHYIISKNLDVEVRYDI
                     VSIIIYNGRHELKFIEDAFYPYT"
     gene            complement(14971..15597)
                     /locus_tag="PKY09_05830"
     CDS             complement(14971..15597)
                     /locus_tag="PKY09_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNB12411.1"
                     /translation="MDNIAETLEFKSSPALREKLISYGTTKKFNAGDVILNENAYIRS
                     IPIVLSGSIRVMRNDDEGREILLYYIKSGESCIMSFLGGIHQDTSKVRAVAEDETEIL
                     FVPVDKVITLIREHDDWLDFIFRLYHKRFEELLEVVNAVAFKKIDERLILLLQKKAEL
                     SKSKTITVTHEQLANELGTARVVVSRLLKQLEVDGRVELGRNKITLLQ"
     gene            complement(15630..15962)
                     /locus_tag="PKY09_05835"
     CDS             complement(15630..15962)
                     /locus_tag="PKY09_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12412.1"
                     /translation="MTSSQPLYTDNDFLRLITHCTSADEVKQVLSSHNVADAESKSSE
                     LFKKLCCIKRQTKGIYLMAAGALMGFIGCLLSIVNPIPFLFDIALYGLTPGAVVVVLI
                     GLYLFIEG"
     gene            16094..17035
                     /locus_tag="PKY09_05840"
     CDS             16094..17035
                     /locus_tag="PKY09_05840"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNB12413.1"
                     /translation="MKRTITPLVVLCLMAATSFAQTTIVLQPGPADGKDAEIFSCVSC
                     GYAGNNYGTKRDLNAIAWTKNGNNSNVRSLIQFDLSAIPANAFINSATLSLYFNPTSE
                     EGKHYCFPHSNSSYLERITSNWSETTVTWNNQPTTTTQNRVSLGGTTSSTQSFTNINV
                     KQLIIDSRNNPSTSFGFMLRLQQEHKFKKLIFASSDNATAAIRPKLTITYTLPAPVND
                     GHPMARLDGGQTFASISIFPNPVSGTATIQLPDNTENGAVKVEVFNMLGKLCNRMVTY
                     VNTDRIVNFDATTLQQGVYVVKVFCNEKVYTQRIVVK"
     gene            17206..17985
                     /locus_tag="PKY09_05845"
     CDS             17206..17985
                     /locus_tag="PKY09_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNB12414.1"
                     /translation="MSSENFEVKLPAFEGPFDLLLFFIERDELDIHNIPISKITEDFL
                     NYIHNLNALNIEVAAEFILVASTLMRIKAKLLLPRPQVDEQGNEIDPRHDLVQQLLEY
                     KKYKAAAEEFRFMEEERAMKFVRGNLAKEAAKIAVVADSSGFSSELHQLTMYKLIAAY
                     QRVLQRFELSQLDLRHTVVQYPFTLESEKEGIIRKISTKASVSFEELFESCTSKIHAV
                     FIFLALLDLLQIHEVSINVGEGFNNFILQKPQDLFTHADRE"
     gene            17969..19030
                     /locus_tag="PKY09_05850"
     CDS             17969..19030
                     /locus_tag="PKY09_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB12415.1"
                     /translation="MQIESKDVLRRLNPTKIVWPMLIGLSVAGWLFYDNYDSKVFSQI
                     DWTWHSTFWMILAGFSVFVRDLAYMIRIRVLTDNELTWYRSFIVIMLWEFSSALAPGI
                     IGGGFLFAIFILNREGINLGKSITTITFTSFLDGVFLAVMAPVVYLLVGKEALFSGVN
                     VGQTSLGTSIFYSFWVVYFIILAYKLFIAYALFVNPYLIKKILTGICSFPVLKRFRKG
                     AVETGDQLIIASNGLKDKNLKYWMLSLLATFASWTARYSIVNCLIHAFHGQTALSDLI
                     VYGKQVIMGIIILLSPTPGGSGIAELMFTDFLGEFIHKGLAPTLGILWRLISYYPYLI
                     VGALILPRWIRERIVKKIF"
     gene            19035..19247
                     /locus_tag="PKY09_05855"
     CDS             19035..19247
                     /locus_tag="PKY09_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12416.1"
                     /translation="MSNKRRIFLIVISITIGMGLSVIIFLMKNKGKALSEMDYLWLGT
                     NLFFSLLIVGGIGLYFMKKKDNDLRK"
     gene            19283..20074
                     /locus_tag="PKY09_05860"
     CDS             19283..20074
                     /locus_tag="PKY09_05860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNB12417.1"
                     /translation="MKKQTLVVAATLIFSLMTFAVYAHNEGKQITYQSGNTNCKGYVA
                     YNAHIPKMPVVLVIPEWWGCNEYAKMRADMLASMGYFAFAVDMYGDGYIAPTPKEAGE
                     RASKFYTDNALAMQRIDAAIQKLKDFPMADVENMAIIGYCFGGSMALNAARANLPVKA
                     VVSFHGGLKGIANPAEIKPKILICHGGNDNFVPADEVTAFKSEMDKVKVSYRFIDYPG
                     ATHAFTNPEATAVGKKFDMPISYNAEADKKSWMDMKAFLAEYLKK"
     gene            complement(20076..20864)
                     /locus_tag="PKY09_05865"
     CDS             complement(20076..20864)
                     /locus_tag="PKY09_05865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB12418.1"
                     /translation="MDYLDLNKKLWNERTVVHVDSAFYDNETFLKGKNSLNEIELNLL
                     CDVKGKTILHLQCHFGQDSISLARLGAQVTAVDFSEKAIETAIELNKKSGTDCKFICS
                     DVYSLNNVLDQKFDIVFTSYGVIGWLPDMQKWASLISSFLKPEGVFVIAEFHPAVWMF
                     DNDFKYVQYSYFKSEPIIETDEGTYADKDANITTISVTWNHSLSEVINSLINSRLRID
                     SFNEFDYSPYNCLNNMVEVSENRFMIKGMEKKLPLVYAIRALKQ"
     gene            20950..22008
                     /locus_tag="PKY09_05870"
     CDS             20950..22008
                     /locus_tag="PKY09_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB12419.1"
                     /translation="MSHILLIEDNKEVRENTAEILELAGYTVTTANDGKKGIEAVHKN
                     RPALIICDIMMPVLDGYGVLHLLGKNPETSTIPFIFLTAKTERGDFRKGMEMGADDYI
                     TKPFDKLELLNAIESRLKKVEALKKEYSKDLEGLTNFIGEVGGKDHMQAFIENRKIRT
                     YKKKENIYKEGDYPRGMYYINHGKVKLYKSHAYGKELITNLLKEGDFFGYTSLIEEGD
                     YVESAQTLEDTEVVFIPKEDFLNLLFSNREIAKRFLLILNKKFNEQQDYLIELAYSSV
                     RKRVAEALILLSDKYKANRDERFSMSISREDLANIVGTATESLIRTLSDFKEEKLIEV
                     KDGKIVLINENKLKELKN"
     gene            complement(22044..22748)
                     /locus_tag="PKY09_05875"
     CDS             complement(22044..22748)
                     /locus_tag="PKY09_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNB12420.1"
                     /translation="MFKTLLTIALGISFSFAGAQTAKQDTVMTPEPDKAGLYDCRVNF
                     PDGRARAVGKMLNGKKEGLWRFYDTKGKIELLEEFEADVRNGLHLHFNENGYIEDEES
                     YRKGILHGLRNVYKYGVTLITSENYNNGKLDGVRFDYYENGKLKEKATYKNGIRDGIT
                     IWFKQDDKPTIQYTYKNGDLNGPAIFYANGEIQSEGNYVNNNEEGEWRVYEDGKLLKT
                     VLYKNGKVVKETAAKK"
     gene            22840..23928
                     /gene="prfA"
                     /locus_tag="PKY09_05880"
     CDS             22840..23928
                     /gene="prfA"
                     /locus_tag="PKY09_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNB12421.1"
                     /translation="MTTQEILEKLEALKHRFEEIGQQLSDPSATADMRRFQQLSRQYK
                     ELQEVVETYERYKKVIDNYDQAYHVLNTEKDADLKQMAREEMETLEAEKNMLEEQIRL
                     LLIPKDPIDERNAVVEIRAGTGGDEASIFAGDLYRMYSRYFEKKGWKVDVIDENEGTA
                     GGFKEVIMEVSGDDVYGIMKYESGVHRVQRVPDTETMGRVHTSAATVVVLPEADDVDV
                     VINPADLEYQTARSSGAGGQNVNKVETKVQLTHKPTGIMVTCQIERSQHGNRERALHM
                     LKTKLYEIELQKHESEIAARRKTMVSTGDRSAKIRTYNYPQSRVTDHRIGLSSYNLSS
                     FMNGDIQEFIDALQLAENAEKMKEASLS"
     gene            23932..24756
                     /gene="pyrF"
                     /locus_tag="PKY09_05885"
     CDS             23932..24756
                     /gene="pyrF"
                     /locus_tag="PKY09_05885"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNB12422.1"
                     /translation="MNYQQLVGEIKRKQSFLCVGLDTDLKKIPPHIREKADDPVFEFN
                     KAIIDATLDYAIAYKPNLAFYEALGVEGLKSLEKTIQYINSRAFTIADAKRGDIGNTA
                     EMYAHAFYNQYGFDSITLSPYMGADTVEPFLKHKDKWSIILALTSNPGADDFEMLKID
                     EDYLFEKILKTFSTAATSQQVMFVVGATRPQYFEIVRKHAPDNFLLVPGIGAQGGSLE
                     EVCRYGLNSNTGLIVNSSRQIIYAGSGTDFDEKAASQAKQIQQQMKQILVEFKITG"
     gene            24756..25490
                     /locus_tag="PKY09_05890"
     CDS             24756..25490
                     /locus_tag="PKY09_05890"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HNB12423.1"
                     /translation="MPFTLAHPAIVIPFYRLLKERLSLTGLIIGSMVPDVEYCVNTVT
                     RSVISHTERGIWMFDLPVAIFLTFCYHGFVKQTLTNNLPAWIRYRLAPYANQNWFHYF
                     RKNFLNYFLCLLAGIILHLLWDSVSHGTGYFVRRSAFLQENVIGTLTVTRLIWHISTV
                     VGLYVVIRFFLNFPVSKVEAENNKPDKNFWPLVFLVASVLTIIHWLPFVRPQEPRFIL
                     VAFSGAMMVGVIVVSSIFRFYKKQLS"
     gene            25487..26761
                     /locus_tag="PKY09_05895"
     CDS             25487..26761
                     /locus_tag="PKY09_05895"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNB12424.1"
                     /translation="MNEALLYHLWKYRLFKSMPLTTIDGKTIEVIKPGELNPDSGPDF
                     FNARLIIDGTEWAGNVEIDPTSADWYNHKHHINKDYSKLILHIVYELNKPLDHGFPVL
                     EFKNFMDEGAVRTFHHLQESPLPIPCSKQIKQISDITLNTWLERMIIERLEQKTAAIH
                     DMLQVNKFDWEETFYFFLARNFGFKTNALPFEMLARSIPMTALARHKNNLLQIEALLF
                     GQAGFLSDKHTDEYVESLRREYRFLAGKFQMKPMNNSLWKFARMHPRNFPSVRIAQFA
                     SLIHNSTHLFSKILEEKDVKSLIQLFSFTPSSYWDKHFHFGKESAENKKTFGRDSADN
                     IIINTIAPFLFLYGKIKDNEKLTERALSLLEHIKPENNVIIRQWKSAGITAKHAYHTQ
                     ALIQLKNLYCSKKKCLSCAIGVKILNNKSNDH"
     gene            26757..26975
                     /locus_tag="PKY09_05900"
     CDS             26757..26975
                     /locus_tag="PKY09_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNB12425.1"
                     /translation="MDSIKNFFERQAFGICEYWGERLKIRSSVIRLFFIYLSFLTIGS
                     PVVVYMVLGFWMNLRKLIHRQRGGVWDL"
     gene            27013..27387
                     /locus_tag="PKY09_05905"
     CDS             27013..27387
                     /locus_tag="PKY09_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNB12426.1"
                     /translation="MIKMYIKPTCSTCRTAVQLAKACDVSVEMVEYLIDTPSEKELLE
                     LTQQLGVKPFDLVRTKESLYKDKYEGKKISDKQWLKILHKNPILIERPIITDGTHAVI
                     GRPPTLIVDFLNQKQKTKKKSK"
     gene            27421..>28582
                     /locus_tag="PKY09_05910"
     CDS             27421..>28582
                     /locus_tag="PKY09_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNB12427.1"
                     /translation="MQSVIRKLFIAVLLLSAVQVNAQQDELFIPRNIQKAYQNKTRNL
                     QGEPGSKFWKNYAEYYIRVSFDPKTLKIKGEQVITYYNNSPDTLKKLVFRLAPNFYKK
                     GNERNRIINPEDVTDGVDISRCVIDAKEINIHDKHVVKQKGTILEIKIDPLSARGGKT
                     TVEMNWSYTLNNGSHERTGVIDKGSYFVAYFFPRITVYDDIDGWDDSPYIGQQESYFD
                     NANFNVFISVPRNYMVWATGDLQNPSEVLHESVLKKWTVALSSERPVFVIDSLDLRDK
                     TVTLQKKVNTFQFKATTPDFVFALSDHYLWQARNMVVDSLTMQRAFITTAFNPVHKDF
                     FEVNEFAAKTIETMSFDFPGVTYPYSHLTIIDGLDQMEYPMMINDNPTTTRFD"
BASE COUNT         9218 a         5171 c         5231 g         8962 t
ORIGIN      
        1 tatatcatac tttgtgtacc agtcataagc aaagccatca ctcattttgt tctcaatgcg
       61 agcggtaaat acgcccggtt gcacaaggga gcctaagtcc acttctccaa aggcagtgtc
      121 aatattcggt gaaactattt tagggcaact tggaggaggg ttaatgtata aataacagga
      181 atccatgtaa tgccctatgt aaaccgtatc tcttgcgcct gtagcatctt caaaagcaat
      241 ggttagcagt tcgcttggcg ttacgcttcc ctgtgataat actcttgctg cataagcaga
      301 cagtaggata ataagtaaat attttttcat tgttttaaaa ttttggtata aaaattaaat
      361 ttaaaaccct gtagttacca taaggtgaaa taccggatcc atttttacta cagtaagtaa
      421 aattgcccga acacgtgggt gctgatacag tcccgtttac acgcagtgca gtagaatctt
      481 caatatactc gcattgggca aatagtaatt tgcctgttag cattagaaca ataataattt
      541 taatttctct cataagattt gtttatttaa aagttgaaat aaaatttaat tatatttaat
      601 cgcttttaac aataatagca ttgctgctgc ttttatcggt taacacattt aataaatacg
      661 taccactttg ttgatttgct agggtgatag tatttatgcc cgctggtaaa atggttctta
      721 aaattacctg tccggtaatg gagctaattg ttactgttgc tttttcaatc acatttattt
      781 gtacagtttc agtaaaagga tttgggaata ctttaacgga tgagtttgta taagtatcat
      841 gtaagccagt aagaggttgg ctccatttaa aaagataagc atcgtccgtg cctgttgatg
      901 ttaagttgta caaattattt gaaggatcaa aatcgcatgt cccgccaaac tctccagcta
      961 cgtagatttt ttcatgtgca tccaatttta ttgacaatcc aatattgttt gaacctttta
     1021 tttgattagc ccaaattaaa ctaccggtac tttctaattt actaatgaag gtatttattc
     1081 ctccgatact acttaaatta taaataccag ttccaggatc aaaatcaaca gtatcgttaa
     1141 atgcaccagt ggtataaatg ttatttactg agtctataac tatccccata ccctcatcac
     1201 ttcctgtact cccccaactt tttgcccaaa tcaaattccc tccattatct aacttaaata
     1261 aacaaacatc agtcaaacca ttgcttatta aattaaaaac atttgcactc ggatcagcat
     1321 ccaaggtatc tataaaataa cctataccat aaatattatc taaattgtct atggttaaat
     1381 tacttccaat tgaatagtta gcccccccca attgttttgc ccaggcaaaa tgccctccac
     1441 tgtctaattc gcagatgtaa atatctgagt agccatagga taataaatca taattgatga
     1501 ctgtatccgg attgaagtcg cataaacctt taaactcacc tactaaaaga atatctccgc
     1561 tactattcaa tttaatagaa tttacatagt cttcatcacc accaccaaaa gtttttatcc
     1621 aattcaaatt accaccacca tccagtttag ctataaaaac atcactaatt ccttttgaat
     1681 ggcccataag gctatcattt gtgatatcta taaagtgtag ggtatctgaa aaaataccag
     1741 cccaaaacac attgccatcg ctaacaacac aagaagttcc tctatcatat gaaaattcac
     1801 tgccactcac tacttttacc caaataaaat tacccgtaga atctaatttt agcaaaaacg
     1861 cacttgaagt actttgggat tttaataaag taactcccgg gcccggatca aaatcaacag
     1921 agtccccaaa ataaccagaa atgtaaatat tactgttatt atcgcatacg atctcatagc
     1981 taccatctac acctgggcct ccaattaatt tattccaaag aataacgcca ttagaatcta
     2041 ttttataaat aaaacaatcc ggactggaag cttcagtaaa tccagtgaca taaatatttc
     2101 ctgcttggtc aatagcatta ctagtggcta tgtcaacttg aatgccccca aactgcttag
     2161 cccattgcag ggtttgtgaa gtagcaatag aaaattgcaa aaccaaaact attaaaaaaa
     2221 gaaaaaagtt tctcatggtg tttacttgag aatttaaagg tataaaattt ttttaacagt
     2281 caaagctcca aaacttaatt cataatattt tcttgtaaaa ataccctgtg gaggtacggt
     2341 tgcaaaatca gcatccatga aagaggttaa attagtaggt ggattggagt ttactgtatt
     2401 cgatgtgccg ggatcccaat aattactagg ggcaccagca agtggatcat ttggtgtagc
     2461 atcataaata atattgatca ttaaatttaa aactctgtag ttgccataag gcgaaatact
     2521 gaatccgttt ttactttcat aagtagcccc ttgtaatgca gaaggcggag acgggtcaac
     2581 aaattcacat tcctgtgcca ttgcattttg gttcagcaac aatactgttg ctattgcgct
     2641 aattggtttt gataatcttt tcatgatatt gattttttga gttatctgta atttgtatta
     2701 tataaattcc atttgttaag tcatttatct gaaatgaata ttgtttatta taaatattag
     2761 aatatttttt aataatattt ccgtataaat caaacacctc tattaacgta atcgacttct
     2821 ctgatttgat aagtacacct gtggttgaat tagaataaat gcttatatct tcattttcta
     2881 taaaattttc acctatgcca attgtttcat ctctcataat ataaaaaccc attggaaact
     2941 gagatgctgc accccacgag aacgatggag ctaatacctg attgtcccaa agcatattaa
     3001 atacgtgaga tggcgggtca ttattaactg caaaaaaata atcattatta ataaaagccc
     3061 tgtttatata cccaacaggc aagggaagtc ccggaacatg aaataatgag ctgtcccatg
     3121 aaaccattat ggggtattgg taattaattg cgtaaacaat cacatcgttt gaagagtgtg
     3181 ggtggggtaa tgcctgtgtt tttgtagtat cgccattggt atttcctata aaaacattaa
     3241 actgattata attcaaacta acagcctgtt cgttaaattg gatatcaact ccaaaagttg
     3301 ctgttgtatc ccaaatacac caaatggtgt ccttagctcc ggtagcatct tcaaatgcaa
     3361 tgtgaaattt ccattggggt tgggcaaaag cctgtatgga aaagaaaaca caaaacaata
     3421 ctgttgctat tgcgctaatt ggttttgata atcttttcat gatattgatt ttttgagtta
     3481 tctgtaattt gtattatgta cattccgttg ggaatatctt tcattgaaat aataaagtca
     3541 tcagaattta tttgcctgta ttctttaaac actgttcctg ttaaatctga tagctgaatg
     3601 ttatcaacat gatttttaat actgattctt aaattatctg ttacaggatt agggaatatt
     3661 ttatattgct cttttgatat ggtattaagt cctattgttg ggtcataaag caaattaatg
     3721 tctaatggaa attggcttgc gcttccccac gcgaaagcag gggcaaacgc atgatcatca
     3781 atcatcatat caaaataatg gcaaccaatg caattattga tcgcaaaaaa gtaatcgtta
     3841 tccatcctgg atacattaaa tattccttgc tgaacaggaa gatatgatgc atgaaagagt
     3901 gatgtatccc aacttacagt tatcggatat tgataattaa aagcatttat atttgcagaa
     3961 tgctcaggat agtataaata aggtaaagcg tatgtttttg ttgagtcgcc aatatcattg
     4021 tacagccaaa cattaaattt agagtaatca aaaggatagg ctccctcacc aagcgcacta
     4081 tcaacctgaa tagggaaacc tcctgtagca gaagtatcat aaaccagcca aatagtatct
     4141 ttagcccccg taacgtcttc aaatgcaata tgaaatttcc attggggttg ggcattaagc
     4201 atggaggctg taaataaccc aaaagcaaat tttatgagta acagtttttt cattattcta
     4261 taattttgga ataaaaataa aatttaaaac cctgtagttg ccataaggcg aaatactgta
     4321 tccattttta ctacagtaag taaaattgcc tgagcatgcg ggtgctgata cagtaccgtt
     4381 tacacgcagt gcagtagaat cttcaatata ctcgcattgg gcaaatagta atttgcctgt
     4441 tagcattaga ataataataa ttttaatttt tctcataatg tttgttgatt taaagttaaa
     4501 taaaatttaa ttttattaat cgcttttaac aatgacagca ttgctgctgc ttttatcggt
     4561 taacacattt aataagtacg taccactttg ttgacttgcc aaggtgatag tatttatgcc
     4621 ggcagccaaa atggttctta aaattacctg cccggtaatg gtactaattg ttaccgttgc
     4681 tttttcaatc acatttattt gtacattttc agcaaaagga tttggaaata cttttatgag
     4741 tgtgtttgta taattatcat gtaaacccgt aataggttgg ctccatttaa aaatataagc
     4801 atcatcaata cctgatgaaa tcagattgaa tgaattattt gaagggtcaa aatcgcaata
     4861 accatgaaaa ccaccagcca catatatatt ttgattctta tctaaagcta ttgatgttcc
     4921 aacattatca gttgtcccag ttaattgatt tgcccacacg aaattaccgg cactgtctat
     4981 tttactaaca aacgtgtccc ctgaactatt tccaaataaa ttgtaaacac ctacacctgg
     5041 atcaaaatca acggtatcag caaaccgtcc agtagtatat atgttggcag atgaatcaag
     5101 agaaagtttc attcccatat cagttcctgt accccccaag ttttttgccc aaatcagttg
     5161 cccattagca tctaatttta caagaaaaac atcacgattc ccatggcttg acaaattaaa
     5221 taccgtgcta cctgggtcga catccataat tccagagaaa tagccagaaa ttattacatt
     5281 accaagttgg tcagataaaa tatctgttcc attaccgtgt tgcgctcctc caaactgttt
     5341 agcccaaata aaattgccag cactatcaaa tttacaaaca taaacatcaa actgcccaaa
     5401 tgaagtcaaa ttaaaattta ctgtagtatc aggattaaaa tcacacagtc cattaaacat
     5461 acctgttaat aaaagattgt tagtgttatc tatagctatt gctgcaattt cttctctttg
     5521 aatacccccg atggttttcg accatagaat attaccacta gaatccattt tgagtacaaa
     5581 aacatcttca tatccatgag atactgctat gggtaaagaa atatttggtg ccagatgtaa
     5641 aagggtgtct gtaaaaattc cacaccaata aacaatgtta tctttaacta tacacgaccg
     5701 agagtgatca tcatcaatag atttgtcacc tccaagctgc cttatccata taaaattacc
     5761 tgcagaatct aactttagta aaaaagcatc ataaccgtgt gaaataagca tgcaggtgtc
     5821 aggcccagga tcaaaatcaa cagaatcact aaatgaaccg catatataca cattacaaat
     5881 agtatcagta gttatatgaa aactttcatc tacacctggg ccaccaatta ttttactcca
     5941 aagagtatta ccattggaat ctactttatt gataaaaatt tcagtatcag tataacctgt
     6001 aatgtaaata ttaccagtaa agtctatggc attactcaaa gctgcatcag gctgcgcacc
     6061 cccaaattgc ttagcccatt gcagggtttg tgaagaagca atagaaaatt tcaaaaacaa
     6121 aactatcaaa aaaataaaaa agtttctcat ggtgttttat ttactgcatc taaagtataa
     6181 tattttttca ttgttctata attttggaat aaaaataaaa tttaaaaccc tgtagttgcc
     6241 ataaggtgaa attggagatc cagtatccaa ctattttatt tcggaagaaa ttctgccaga
     6301 agttaagact cttccatatc cttggattca gcattcttct attgatgtga acaaacaaca
     6361 gtattaagtt ataaagcact catcattatt attttttttc catcatctct tattttttct
     6421 tacaagcaat tttcaagctt gacggtagtc tataacatgt ctctcctctg aaagaagaaa
     6481 taagtcctta cggttagctc aattttctta caaaagaaag aatgcccatt tcgatgtcag
     6541 aaaatgttga gtagcggcaa ttggtttgca attatccata caaattcaaa taaaaaacaa
     6601 tatggactta ttaatctcaa ttttacttta cttaggtgtt tatgctacac cggctgatct
     6661 tcagaataaa gagtttgttg ctaaaaattc tgaaaaaata aatcaagcag aaacgctttc
     6721 aaaagatcca aattttaaat gcgatggaat tgtaattgtc ggcagtggca atttgtaatt
     6781 aacatctcca aaaaatatca aaggcactga cttcaaaaga tcagtgcctt tttaattttt
     6841 ctaaaatggc tgcccaaagt ttttcataag gaataacttc aagcccttct gtattaaaaa
     6901 atttattgcc ggttacaggt tttagttttt ccaaggatat ggcagtttgt ttctgtgtct
     6961 tagttttttg aaagttgttc ttgaataatg tgagtaacat attcagaaaa ataaatgtac
     7021 gcggataaat tctcctgtca acatattttt tgatatatga ttccagaaaa cttgttctgt
     7081 catataactt gtcataatct cccatttcaa tcatcactat tatctgacct atcatgataa
     7141 taatgttgtt gtcttcttta tgagttgtgt agtagctgat ttcatctaaa aaacccaaca
     7201 ggtttatact ttcttttgga aaatgacccg gtaaaataaa attaagataa ggttcaaaat
     7261 aatgccaacg ctcttttact tcctgattgg agttgataaa atattcactt cgtgttgctt
     7321 cgtaataaat gtttaatgcc tgttcaaaat ttccggagtg catacaaaga ataaaataat
     7381 gctcgagtgc atgtagccag tcagatgagt tggcacttaa cttgcctaaa aaaatccgtg
     7441 cttcctcttt acctctatca tactgcctga ggtggatgta ggccaacatt cttgaaagcg
     7501 caataaaact tttttgaaag tcggaatagt attcgggatg attcagataa aaggcttcac
     7561 gttcattcaa agcttcttct accaattcaa aattactgct gaaatagcca taaataaaag
     7621 caatgttgcc gtaatagaaa tttgaattaa atgttttcag ttgctgatgt attttttggg
     7681 ctctcagata atagcgtttt gctttagcag tatttttata ttgtgaaata tttgaggcta
     7741 cctgaaaaat atccagttct ctctgcatca atatcaactc agcctccaat tcaaactgtt
     7801 tgatagattt tttaaagcgg tttcgataat gataaaaatc gtcaggtttg tttttaatag
     7861 ccatcctgtc ggcaagcatt aggtcggtaa ggcaaccaat catgccatcg tttgtaaacg
     7921 ttgcatagtc tgctgctttt ttaaagaaat ggtcagaaaa aaaagcggag gcattttgct
     7981 gacgtaatat catggcagca ccaaaataaa tataagcacg aaaagaactt cgccccaaca
     8041 tggagttcat cagtttttcg gggtctaaac caaaaaccaa attcaccaaa cgatcttcga
     8101 gttcggcttt cagctgttga taggcaggat ttgttgttgt actttgtaaa agtttttcgg
     8161 cagcttcctg atcgttgttg atactacctt ttgaaatttc cttataaaac cataacagtt
     8221 caggataatt tttatctgca aaataaacat caggcacacc aagaaaatca tgacggtttt
     8281 tcgcaaaaaa attaattaat aatttgattt cagaaaattc catcttttat ccgataaatc
     8341 aatgctttat tttctttcaa atataggtca tcattagatg atgtactgca acgttgagat
     8401 tttatttttc agatggattg ttaacagcct gcattatatt tcgagctttg aacaagcttt
     8461 ttctgctgca atctgctctg cttttttctt tacaaaatcc attcctgttg caacttcttc
     8521 tccgttaatc atcaaccgca ctcttatgag tttatcgttg cttttttcgt cttcaatagt
     8581 ttcaaaggca actgtgtttt ttgttcgctg cgaccagttg agtatgcggc ttttaaaatt
     8641 tatttctgtt gcttccaacg attccaaatc aacatggtgg ttaatcattc tttcgagaat
     8701 aaactgacgg gctttgtcgt agcctctgtc aatataaata gcaccaatga gtgcttctaa
     8761 tgcatcacca taaatgttgc gaaacgaatc ggggccatgt gtttttttaa taaaatgatt
     8821 tatgccaagt ttgaaggcaa gatgttttag attttcgcgg cttacaatct tgcttcgcat
     8881 ctcggttaaa aaaccttctt ctctgaatgg aaatttttta aaaagcattt cggctacaac
     8941 ggcaccaagc acagcatcac caagatattc taatcgctcg tttgaatgtt ttataccatt
     9001 ggtaatttct tttgtttgcg aacgatggct aaaagctaac ttgtaaagcg ataactttat
     9061 tggataaaag ccaagaatat tatgcagcga aaagtatgtt ttgcgatcag ctgaaaataa
     9121 aatattgata ggaaagaagc tgtaaaatct cactgattat cagggagtga attttttaaa
     9181 aattacagag gcattgtgtc cgccaaagcc gaaagtgttg ctcagcgctg ctctgatgtt
     9241 gcgttgttgg ggtttatgaa atgtaaaatt caaccgtgga tcaaaggcag gatcgtctgt
     9301 aaaatggttg atggttggtg gtacaatatt attttgaacg gcaagtatag aagcaatagc
     9361 ttcaatagca ccggcagcac ccaacaggtg tcctgtcatg gatttggtag agctgatgtt
     9421 taacctaaaa gcatcatcac caaaaagttt tagaatggct ttaatctcac ttgggtcgcc
     9481 cacaggtgta cttgtaccgt gcacattaat atagtcaatg tcggcagaag tcattcctgc
     9541 atcgtcaagc gaagattgca ttacgttaaa agcacccaat ccttcaggat gtgttgcagt
     9601 gaggtgatag gcatcggcac tcataccacc gccaaccatt tcggcataaa tttttgctcc
     9661 gcgtgcttgt gcatgaccaa gttcttcaag aacaatacaa ccggcacctt caccaagaac
     9721 aaatccatcc cggtctaaat caaacgggcg tgatgctgtt tgaggtgagt cgtttctctc
     9781 actaagtgca tgcatggcat taaaaccgcc aataccagct tcgttaacag aagcttcaga
     9841 gccgcctgaa ataataatat cggccttgcc caaacgcaga taggtaaatg catcaatgag
     9901 agcattggtt gatgatgcac atgcagatac tgtagtatag tttgggccac gataaccaaa
     9961 acgcatagag atatgtcccg atgcaatgtc ggcaatcatt ttaggaatga aaaaaggatt
    10021 gaaacgtggt gttccatcac ctttagcata agcaataacc tcatcaagaa aagtacgaag
    10081 tccacctatg cccgagcccc aaataacacc tactctgtca tgattaattt tggcagtatc
    10141 taagcctgca tcttttactc cctgatcgga aactgcaata gcatattgcg aaaagggatc
    10201 tagcttacgt gcttcttttt tctctaaata aaccagcggg tcaaaattct taacctcgca
    10261 ggcaaagtgt gttttaaact ttgaagcgtc aaactttgta atctgacaag caccacttac
    10321 accgttgagt aagccgtccc aatattctga aacagagtta cctaatggtg taagggcgcc
    10381 taatcccgtt actacaactc tcttgagctc catgtaaggg acgcttattt tacgttagca
    10441 gtaatataat ctacagcttg tcctacggtt gagattttct cggcctgatc atccggaata
    10501 gcaatattaa actctttttc aaattccata atcagttcaa ctgtgtcgag cgaatcagct
    10561 cctaaatcgt ttgtaaatga cgcttcatta gttacttcac cttcgtcaac acctaatttg
    10621 tcaataataa tggcttttac tctggatgca atgtctgaca ttttatataa agttttgaat
    10681 tagagcgcaa attaaatggt tttgtaactg attttcaaaa atattttgcc caacataatt
    10741 aattgttctt aattgaataa gaggcacata cacacttatc gggcagacaa caatttttta
    10801 acttttcaaa acagggtact aaaaccttct tattccgatg atttgcctta cttcttctat
    10861 tgttttttgt gcacttgcct tagctttttc gcgcccaaga agaaccactt tttttacata
    10921 atcgtggttt ttttcaatgt caaggatttt ttgggtaatg ggagccataa aagtcaccat
    10981 gtcttcggca agctgctttt tcagatctcc gtaacggatg ttgcaattat tatatgaagt
    11041 ttcaaagtgg cttattgtat ctgatgaaga aaccaatttc atcaaactga aaagattttc
    11101 tattgcctcg ggcttggccg agttaggctc tgttggcccg ctgtcagtta ctgcacgcat
    11161 tatttttttt ctgatggtgg ctgcatcatc tttcagaaac aaggcattgc cttcactttc
    11221 cgacttaccc atttttcctg caccatcgag tccgggaact tttactaatg tattgccaaa
    11281 gttaaatgcc ataggctcac caaaatattc tgtcttatat aagcggttaa aacgatttcc
    11341 gaatgtgcgg ctcatttcaa gatgctgctc ctggtctttg ccaacaggaa cttttgttgc
    11401 cttatgaatt aaaatatcgg cagccattaa aacaggatac gtcaatagtc cggcattaat
    11461 attatcagga tgttttctga ttttttcttt gaatgatgtt actctttcca actcaccaac
    11521 ataagcattc atattcaaca gcagatatag ctctgccgtt tcaggcaaat cgctttgtat
    11581 gtacattgta catacttccg ggtctaatcc acaagctaaa tactcaacca aagctgcttt
    11641 tacattttgc tgaagatttt caggtgtagg gtgtgtggtg agtgagtgat agtcggcaat
    11701 aaagaaaaag cagcggtatt catgctgcat ggctgtaaaa ttttttatcg cgccaaaata
    11761 attacctaag tgcaggtttc ctgttgatct tattccgctt actactgtat ccatagctgc
    11821 aaaaatagat taattgtatt aacaggtttt tattttagta aaacagcaaa gaattattat
    11881 ttaatttggg ttataaatat tgattgtaat gataccatta gcagagcgca tgcggccaaa
    11941 atcgctgaat gaatacattg gccaggaaca cctcactcag acaggagcag ttctggcaaa
    12001 agccattgaa aaaaatacca tccccagcat gattttatgg gggccaccgg gtgtgggcaa
    12061 aacaactctg gcttttatat tagctaactc attaagcaga cagttctatc atctgagtgc
    12121 catcaacagt ggtgttaaag aagtgcgtgc agtaattgaa acggcacggc aacattttga
    12181 atctgtacaa caaccgtcaa ttttatttat tgatgaaata caccgtttca gcaaatcgca
    12241 gcaggattct ttacttggcg ctgtagaaaa aggaataatt acactcattg gagcaacaac
    12301 agaaaatcct tcgtttgaag tgatttcacc attgttgtcg cgctgtcagg tttatatttt
    12361 aaaagcactt actgctgaac atctcagaac tattttacat gcggcactaa caaacgattc
    12421 agtacttaaa gaacagaaag ttactattaa agaagatgat gcattgctac gcttcagtgg
    12481 tggcgatgcc agaaaactac tcaaccttct tgaattagtc attcaggtaa atcaacaaga
    12541 cgaaatttgt attgacaata aaatggtaga gcaaaccatt catcaaaaga tggcgcttta
    12601 tgataaaaca ggcgaacaac attatgattt gatttctgca tttattaaaa gcatcagagg
    12661 aagcgacccc aatgcagcag tttactggct tgcaaggatg attcatggtg gcgaagatcc
    12721 gttgtttatt gcaagacgaa tgctcattct tgcttcggaa gatattggta atgcaaaccc
    12781 taatgccatg ctcattgcta atcagtgttt tgatgcagca cataaaattg gttttcctga
    12841 atgcaggatt attttatcgc aggcagcaat ttatcttgcc tgttcgccaa aaagtaattc
    12901 aagttatatg gccattgaag aagctttgga gttagttgcc aaaacaggcg accttcctgt
    12961 gccactgcat ctgcgaaatg cgcctactaa cctgatgaaa gaaattggat atgggaagga
    13021 ttatgcttat gcgcataatt atgaaaataa ttttgtgaaa ctaaactacc ttcccaatca
    13081 attaaaaggc aagaaaattt attttcccgg aaaaaatgtt cgagaagcag agtttcaaca
    13141 atttttaaaa acaaggtggg agaatgaata tgattactga caaaaaataa attgtttaac
    13201 aaaagttaaa atgaaagaca tttaaaatat ctttgtttat atccgtttta aagaaaataa
    13261 ccaacatcat tttatggaaa acacctcgca ttcctttttc gaccgactca atcaatggat
    13321 aaaaaattct gtcatggtga aactcatcag catgggaatt ttaattttat tattacttat
    13381 accatcagaa atggtgcaaa gtttgataaa tgaaagacaa caaacacatg acagtgcatt
    13441 agacgaaatt tcatccaagt ggggcttcga gcaacacctc agcggtcctg tgattaatgt
    13501 gccttatcaa agtttttttg ttgatgcaga tggaaaaaga aaatcgcaaa ttcaatatgc
    13561 tcattttttg cctgaagaat taaaaattgc aggaacactg gagcctgaaa tgcggcatcg
    13621 cggcatttac gaggctgtag tgtataagtc agatgtttcg ctaaagggat attttcgttt
    13681 tcctgatttt gctgaatgga atattgctcc atcggatatt ctgtggaaag agactacgct
    13741 atcattaggt atttctgatt tgcgcggaat caaacaacgt cttaccatca atctgaatgg
    13801 aaatcctatt gagtttaatc ccggagtaga aaataatgat gtgcatgaat cgggtgtaag
    13861 catacgttta ccaatagccg attcgctcaa taaacctgat tttaaatttg acatgtcttt
    13921 taaactcaat ggcagtaagg aaatgtattt tataccattg ggaaaggaaa ctacagttag
    13981 cataaaatca gactggacaa atccgagttt tgatggcgca ttcctgccag acacacatca
    14041 catcaccgat aaaggctttg atgcagaatg gaaaatattt catctcaaca gaaactacgg
    14101 acaaaaattc agaggtgcgc caaccggatt gcaagaatct tcgtttggtg ttaagctgct
    14161 tattcctgtt gacaattatc agaaaacata ccgctctgcc aagtatgcta ttttattaat
    14221 caccctatcg tttacacttt tcttctttat tgaagtatta aacagaaaac gcatacatcc
    14281 gtttcaatac attttagttg gtgttgcttt atgtttgttt tatactttgc tattgtcagt
    14341 ctcagaatat atcagtttta atttatcgta tctgttatct gcaactgcaa ttattttact
    14401 tataagcctg tatgcaaaaa caatatttaa taacctgcgc ttagcactgt tgtttagtct
    14461 ggtgctgata attctgtatg catttgtgtt ttcaatcata cagttacagg actatgcatt
    14521 gttgctcgga agcttgggtt tgtttattgt gcttgcattg gtaatgtact tgtcgcggaa
    14581 gatcaactgg tatggaacag aaaaataaag gagatcatta tcagcttggc cgcgaaggcg
    14641 aaaacatggc agttgaatat ctggttgcca atggctatca gatagtagaa cgtaactggc
    14701 gtttcggaaa attggagatt gatataatag catctaaagg caacctgttg tgcattgtcg
    14761 aagtaaagac cagaacggga gattattttg gagagcctga agaaggggtt accaaagcca
    14821 aagaacgttt tctggcaaca gctgccgacc actacattat ctcaaaaaat ttagatgtgg
    14881 aggtgcgcta tgatattgtt tccattataa tttacaatgg ccgacatgaa ctgaaattta
    14941 ttgaagatgc tttttatcct tacacctgaa ttattggaga agtgtaattt tatttcgtcc
    15001 aagctctaca cggccatcaa cctctaattg tttcagcaag cgcgaaacaa ctacgcgtgc
    15061 agtacccaac tcattggcta attgctcatg agtgacggta atggttttag actttgaaag
    15121 ttcggctttt ttctgaagta ataaaatcaa tcgctcatca atttttttaa atgctactgc
    15181 attcactacc tcaagaagtt cttcaaaacg tttgtgatac aatctgaaaa taaagtcaag
    15241 ccagtcgtca tgctcgcgaa taagtgtaat tactttgtca acaggtacga ataaaatttc
    15301 tgtttcatct tcggcaacag cccttacctt gcttgtatcc tgatgaatgc cgcccaaaaa
    15361 agacatgata caactttcgc cacttttaat atagtaaagt aaaatttctc ttccttcgtc
    15421 atcattacgc atcactctga tggagccgct aagtacaata ggaatagaac gtatgtaggc
    15481 attctcgttt aatattacat cgccagcatt aaattttttt gttgttccat acgaaatcaa
    15541 tttttcgcga agtgcaggtg atgatttgaa ttcaagcgtt tcagcaatat tatccataca
    15601 gcaaaagtaa gcaatgttgt ttcagcaatt cagccttcaa taaacaagta taaccctata
    15661 agtactacaa ctacggcccc cggagtaagt ccgtatagtg caatatcgaa caaaaaaggt
    15721 attggattaa ctattgaaag caaacagcct ataaagccca ttaaagcacc tgcggccatc
    15781 agataaatac ctttagtttg tcgctttata caacacaact ttttaaataa ctctgacgat
    15841 tttgattcgg catcagcaac gttgtgtgat gaaagcacct gttttacctc atcggcagaa
    15901 gtgcaatggg taatgagtcg gagaaaatca ttatctgtgt aaagcggttg gctgctcgtc
    15961 atatttgtgt tagtgtttaa tgaggataaa tgctatggta agggaaaatc aaaaataatt
    16021 aaaataagtc aaaaaagtgc agttagtttc aaaaaaagtc tttactttgt taccaattac
    16081 aaaaaccatt attatgaaaa gaacaattac cccacttgtt gtactttgcc ttatggcagc
    16141 aacatcattt gcacaaacaa ccattgtatt acaacccgga cctgccgatg gcaaggatgc
    16201 agaaatattt agttgtgtta gttgcggata tgccggcaat aactatggta caaaacgtga
    16261 ccttaatgct attgcctgga caaaaaatgg caacaactcc aatgtgcgtt cacttattca
    16321 gtttgatttg agtgccattc ctgccaatgc atttatcaat agtgcaacac tatcgctcta
    16381 ttttaatcca acctcagaag aaggcaaaca ctattgtttc ccacatagca attcaagtta
    16441 tttagaacgc atcacttcca actggagcga aacaactgtg acatggaaca atcagccaac
    16501 aacaacaaca caaaacagag tttcgttagg aggtaccaca tcttcaacac aaagttttac
    16561 aaatattaat gtaaagcaac ttattattga ttcaagaaac aatccttcaa caagttttgg
    16621 atttatgctt agactgcagc aagaacataa atttaaaaaa ttaatttttg catcgagtga
    16681 taatgctacg gcagccatca ggccaaaact aactattact tacactttgc ctgcaccggt
    16741 aaatgacggt catcctatgg ctcgcttgga tggaggacaa acttttgcat caataagtat
    16801 atttccaaat cctgtttcag gaacagctac cattcaactt cctgacaata ccgaaaacgg
    16861 tgctgttaaa gttgaggtct tcaacatgct tggtaaactt tgtaacagaa tggtaactta
    16921 tgttaataca gacagaattg taaattttga tgcaacgaca ctgcaacaag gcgtttatgt
    16981 tgtgaaagtt ttttgcaatg aaaaagttta tacgcaacgc atagtagtaa aataattctc
    17041 tgatttttcc cggcagatct aattttatta aaatatcagt agttttaact gcagagggtt
    17101 atacaatttg ttggagatga aatctaaata aatcattttc atccttccaa acataggctg
    17161 ttttttcgtt tctttgacaa gagaaaaaac cagacatttc tatctatgtc atcagaaaat
    17221 tttgaagtta agttaccagc ctttgaaggg ccatttgatt tgttgttgtt ttttattgaa
    17281 cgcgatgagt tggatataca caacataccc atttctaaaa ttaccgaaga ttttctgaac
    17341 tatattcata acctgaatgc attaaacatt gaggtagctg cagagtttat tttagttgcc
    17401 agcacgttaa tgcgcataaa ggcaaaactt ttattgcctc gtccacaggt tgatgaacaa
    17461 ggtaatgaaa ttgaccctcg acatgatttg gtgcaacagt tgctcgagta taaaaagtac
    17521 aaagccgcag cagaagaatt ccgttttatg gaagaagagc gtgccatgaa gtttgtgcgt
    17581 ggtaatctgg ctaaggaggc cgcaaagatt gcagtagttg cagacagtag tggctttagc
    17641 agtgaattgc atcagctaac aatgtataaa cttattgcag cctatcagcg tgtgttgcaa
    17701 agattcgaac tctcacaact cgacctgagg catactgttg tgcaatatcc ttttacatta
    17761 gaatcggaaa aagaaggcat cataaggaaa atcagcacta aagctagtgt aagttttgaa
    17821 gaactgtttg agagttgtac cagcaagata catgcagttt ttatttttct tgccttactc
    17881 gatttgctgc agatacatga agtaagtatc aacgttggtg aaggattcaa caattttata
    17941 ctgcagaaac cacaggattt gtttacgcat gcagatagag agtaaagatg tcctgcgcag
    18001 gctgaatccg acaaaaatag tttggccaat gctcattggt ctgtcagtgg caggttggtt
    18061 gttttatgat aattacgaca gcaaagtttt ttcgcagata gactggacat ggcatagcac
    18121 cttctggatg attcttgcag gtttctctgt atttgtgcgc gatttggcgt atatgattcg
    18181 catccgagtg cttacagata atgaactcac ctggtacaga agttttatag taatcatgtt
    18241 atgggaattt tcttctgcat tagcaccggg gattatcggt ggcggatttc tatttgccat
    18301 ttttattctt aaccgtgaag ggataaacct cggcaagagt attaccacca taacctttac
    18361 ctcttttctt gatggtgttt tcttagctgt aatggcaccg gtagtttatt tgttggtagg
    18421 caaggaagct ttgttttcgg gagtaaatgt gggtcaaaca tcattgggca caagcatttt
    18481 ttattcattc tgggtagtat attttatcat tcttgcctat aaacttttta tcgcttatgc
    18541 actttttgta aacccctatc tgatcaaaaa aatacttacg ggcatctgtt cttttccagt
    18601 cttgaaaaga tttagaaagg gtgccgttga aacaggcgat cagcttatca ttgcatcaaa
    18661 cggattgaaa gataaaaacc tgaaatactg gatgctttca ttactggcaa catttgcttc
    18721 gtggactgca agatactcta ttgtcaattg tctgattcat gcatttcatg ggcagacagc
    18781 attgtctgat ttgattgttt atggtaagca ggttattatg ggaatcatta ttttactaag
    18841 ccctactcct ggcggtagtg gcattgccga actgatgttt accgattttc tgggtgaatt
    18901 tattcacaaa ggattggccc ctacattagg aattttatgg cggttgataa gttattatcc
    18961 ttaccttatt gtaggtgcat taattttgcc ccgttggata agagagcgga ttgtaaaaaa
    19021 aatattttaa ggttatgagt aataaacgaa gaatttttct gattgtcatt tcaattacta
    19081 ttggcatggg tctatctgtc attatttttt taatgaaaaa caaagggaaa gccttaagcg
    19141 aaatggatta tctttggtta ggaaccaatc tgtttttctc attgctgatt gttggcggca
    19201 taggtcttta ttttatgaaa aagaaagaca atgatctgag gaagtaaaaa tattttatac
    19261 aagcaattta aaaatacaaa caatgaaaaa acaaacttta gtagtagcag caaccctaat
    19321 ttttagcttg atgacatttg cggtgtatgc acacaatgaa ggcaaacaga taacttacca
    19381 atcgggcaac accaattgca aaggctatgt tgcctataat gcacatattc caaaaatgcc
    19441 tgttgtgttg gttatacctg aatggtgggg ttgtaatgaa tatgctaaaa tgcgtgccga
    19501 catgcttgcc tctatgggat attttgcttt tgctgttgat atgtatggtg atggttatat
    19561 tgcaccaacc ccaaaagaag caggcgaaag agcctcaaaa ttttatactg ataatgcttt
    19621 ggcaatgcag cgcattgacg ctgctattca aaagctaaaa gattttccta tggccgatgt
    19681 tgagaatatg gccatcatag gatattgttt tggaggcagt atggcactaa atgcagcacg
    19741 tgctaattta ccggtaaagg ctgttgtgag ttttcatggc ggattaaagg gcattgccaa
    19801 tccggcagag ataaaaccaa aaatattaat ttgtcatggt ggcaacgaca attttgtacc
    19861 agcagatgaa gtgactgctt ttaaatcaga gatggataaa gtaaaagtaa gttacagatt
    19921 tattgactac ccgggtgcaa cacatgcatt caccaatcct gaagccactg ccgttggaaa
    19981 aaagtttgat atgccaatta gttataatgc cgaagctgat aaaaaatcat ggatggacat
    20041 gaaggctttt cttgctgagt atctgaaaaa ataaattatt gttttaaggc acgtattgca
    20101 tatacaagag gcaatttctt ttccatgcct tttatcataa aacgattttc actcacctca
    20161 accatattgt tcaggcagtt gtagggagaa taatcaaact cattaaaaga atctattctt
    20221 aaccggctgt ttattaaact atttattact tcactcaggc tgtgattcca ggtaactgat
    20281 atggttgtga tatttgcatc tttatctgca taggttcctt catcagtttc tattatgggt
    20341 tcacttttaa aataactgta ctgcacatat ttaaaatcat tgtcaaacat ccaaacagca
    20401 ggatgaaatt ctgctatgac aaaaacacct tctggtttaa gaaacgatga aataaggcta
    20461 gcccattttt gcatatcggg caaccatcca attactccat aagaggtaaa aacaatgtca
    20521 aatttttgat ctaaaacatt atttaaacta tacacatcgc tgcagataaa cttacaatct
    20581 gttcctgatt ttttatttag ctctattgct gtttcaatag ctttttcaga aaaatcaaca
    20641 gcagtaacct gtgcacccaa acgtgctaaa gaaatgctat cctgtccgaa atgacattgc
    20701 agatgcagaa tggttttgcc tttaacatca caaagcagat tgagctcaat ttcattcaaa
    20761 gaatttttgc ctttgagaaa tgtctcattg tcataaaatg cagaatctac atgtacaact
    20821 gtgcgttcat tccagagttt tttgtttaaa tctaaataat ccataatgta aaaatagtag
    20881 ttcggcacaa ataccggaat attgtccaat acatcttatc tttgttcagt aaatttatca
    20941 gaagggatta tgtcgcacat tctactcatt gaggataata aagaagtaag agaaaacaca
    21001 gcagagattc ttgaacttgc aggctacaca gttactactg ccaacgatgg caaaaaaggt
    21061 attgaagcag ttcataaaaa cagacctgca ttaattattt gtgacattat gatgcctgta
    21121 ttagatggat acggtgtgct gcatttgcta ggtaaaaatc ctgagacatc aactattcca
    21181 tttatttttc tgacagcaaa aacagagcgt ggcgatttca gaaaagggat ggaaatgggt
    21241 gctgacgatt acattacaaa gccattcgat aaactcgaac tgcttaatgc aattgaaagt
    21301 cgtctgaaaa aagtagaagc attaaagaaa gaatattcaa aagatcttga agggctgact
    21361 aattttattg gtgaagttgg tggtaaagac cacatgcagg catttattga aaacagaaaa
    21421 atcagaacct acaagaagaa agaaaatatt tataaagagg gcgattatcc gcgtggcatg
    21481 tattacatca accatggaaa agtaaaactt tataaaagcc atgcttatgg aaaagagtta
    21541 atcactaacc tgcttaaaga aggtgatttt tttggttaca cttctctcat cgaagaaggc
    21601 gattatgtgg aaagcgcaca aacacttgaa gatacagaag tggtatttat cccaaaggaa
    21661 gatttcctga atcttctttt ttcaaatcgt gaaatagcaa aacgattcct gctgatatta
    21721 aacaaaaagt tcaacgagca acaggattac ctgatagaat tggcatatag ttctgtgaga
    21781 aagcgtgttg cagaagcatt gattttactt tccgataaat acaaagccaa tcgcgatgaa
    21841 cgtttcagca tgtctatttc cagagaagat ttagccaaca ttgttggtac tgcaacagaa
    21901 tcactcatca gaacactaag tgattttaaa gaagaaaaac tcattgaggt gaaagacggt
    21961 aagattgtct taattaatga gaataaatta aaggagttaa agaattaagc attgaccatt
    22021 cagtcaacac ctgattctgt tttctatttc ttagcggctg tttcctttac tacttttcca
    22081 tttttgtata aaacggtttt aagaagttta ccatcttcat aaaccctcca ttcgccttct
    22141 tcattgttgt ttacgtagtt gccttcactt tgtatttcac cattggcata aaaaatagca
    22201 ggaccgttta aatcaccatt cttgtaggta tattgtatag taggtttatc atcttgttta
    22261 aaccagatgg taataccatc acgaattccg tttttataag ttgctttttc tttcagttta
    22321 ccattttcat aatagtcaaa tcgcactccg tctaattttc cgttattata attttctgag
    22381 gtaattagag taacaccata tttgtaaaca ttccgcaggc catgtaatat tcctttgcga
    22441 tatgattcct catcttcaat ataaccattt tcattaaaat gcagatgcaa cccgttgcgt
    22501 acatcagctt caaattcttc aagcagctct attttacctt tagtatcata aaatcgccat
    22561 aggccttctt ttttaccatt taacatttta cctactgcac gtgcccttcc gtcaggaaaa
    22621 tttaccctac agtcatatag accggctttg tcgggttcgg gagtcatcac agtatcttgc
    22681 ttggcagttt gtgctcccgc aaatgaaaat gatattccaa gagcaatggt caaaagtgtt
    22741 ttaaacataa tgcttaatgt tatggcgcaa atctctaaaa aaatgatgaa aagcagatta
    22801 tagtggcatc agcggctaat gcttattttt gcagacacaa tgacaacaca ggaaatatta
    22861 gaaaagctag aagcgctgaa acatcgtttt gaagaaattg gtcagcagct gagcgaccct
    22921 tctgccacag cagatatgag acgctttcag cagcttagcc ggcagtacaa agaattgcag
    22981 gaggtagtgg aaacctatga gcgatataaa aaagttattg ataattacga tcaggcatat
    23041 catgtattaa atacagaaaa agatgcagac ctgaagcaga tggcaaggga agaaatggaa
    23101 acattggaag cagaaaaaaa tatgcttgaa gaacagataa gacttttgct gattcctaaa
    23161 gatcctattg atgaacgtaa tgcagtggtt gaaattcgtg caggcaccgg tggtgatgag
    23221 gccagtattt ttgcaggtga tctttacaga atgtacagtc gttattttga gaaaaaaggc
    23281 tggaaagttg atgttataga tgagaacgaa ggaacggcag gtggcttcaa agaagttatt
    23341 atggaagtca gtggtgatga tgtttatggc attatgaaat atgagagcgg tgttcatcgc
    23401 gtacagcgtg tgcccgatac tgaaactatg ggtagagtac atacttctgc tgctacagtg
    23461 gtagtgttgc ccgaagcaga tgatgtagat gtggtgataa atcctgcaga tttggagtat
    23521 cagacagcac gttcaagtgg tgcaggtgga cagaatgtaa ataaagttga aacaaaagtt
    23581 cagcttacac ataaacctac aggcataatg gttacctgtc agattgaacg ttcgcagcat
    23641 ggcaaccgtg agagagcatt acacatgctc aaaaccaaat tgtatgaaat agagttacaa
    23701 aaacatgagt cggagattgc tgccagacgc aaaacaatgg tgtcaacagg tgatcgctct
    23761 gcaaaaatca gaacatacaa ctatccgcaa agcagagtaa cagaccatcg cattggtttg
    23821 tcatcttaca atttatcttc ttttatgaat ggcgatattc aggaatttat tgatgcattg
    23881 caactggccg agaatgcaga gaaaatgaaa gaggcttcat taagttaaac tatgaactat
    23941 cagcagctag ttggtgaaat aaaaagaaaa caatcttttc tctgtgtggg tttagataca
    24001 gacctgaaaa aaattcctcc acacatcaga gaaaaggcag acgatcctgt atttgaattt
    24061 aacaaagcaa ttattgatgc aacattagat tatgcaatag cctacaaacc caatcttgca
    24121 ttttacgaag cattgggtgt tgaaggattg aagagtctgg agaaaacaat acagtatatc
    24181 aatagccggg catttacaat tgccgatgca aagcgtggtg atattggcaa cacagcagaa
    24241 atgtatgctc atgcttttta caatcaatat ggttttgact ccatcacact ttctccatat
    24301 atgggtgccg acaccgttga gccattctta aagcataaag ataagtggag cattattctt
    24361 gcattgacat caaacccggg tgcagatgat tttgaaatgc ttaaaataga tgaggattat
    24421 ctgtttgaaa aaattttaaa aacattcagt actgctgcaa catcacagca ggtaatgttt
    24481 gttgttggag ccacacgccc gcaatatttt gaaattgtaa gaaaacatgc tcctgataat
    24541 tttctgctgg tacccggcat aggtgcacaa ggtggcagtc ttgaagaagt ttgccgctac
    24601 ggactcaatt ccaatacagg tttaatagtt aattcttcac gtcaaataat ttatgcaggt
    24661 agcggaactg attttgatga gaaagcagcc tcacaggcaa aacaaattca acaacagatg
    24721 aaacaaatac ttgttgaatt taaaattaca ggttgatgcc atttacatta gcgcatcctg
    24781 caatagtaat tcctttttac agactcttaa aagagcgtct gtcactgacg ggtttaatta
    24841 ttgggagtat ggtgcccgat gttgagtact gtgtcaacac agtaacgcga agtgtcatca
    24901 gtcacactga aaggggtatc tggatgtttg atttgccggt agccattttt ttgacgttct
    24961 gttatcacgg atttgttaaa caaacgctca ccaacaattt acctgcatgg attcgttaca
    25021 gacttgctcc ttatgccaat caaaactggt ttcactattt cagaaaaaat tttctgaact
    25081 attttctctg tcttcttgca ggaattattt tacacttgct ttgggactct gtttcgcatg
    25141 gtacaggata tttcgtcaga cgttcggcat ttttacaaga gaatgttatt gggacattaa
    25201 cggttacacg attgatatgg cacattagca ccgttgttgg cctttacgta gttatcagat
    25261 tttttctgaa ttttcctgtc agcaaagttg aggcagaaaa caacaagcct gataagaatt
    25321 tctggccatt ggttttttta gttgcctctg ttctcactat cattcactgg ctgccttttg
    25381 tccgaccgca ggagccacgt tttattcttg ttgcattttc cggtgctatg atggttggag
    25441 ttattgtagt aagttctatc tttaggtttt ataagaaaca actgtcatga atgaagcgtt
    25501 gctttatcat ctgtggaagt acaggctttt taaatcaatg ccgctaacaa ctattgatgg
    25561 taagacaatt gaagttatta aacccggaga gctcaatccg gactcaggcc ccgatttttt
    25621 taatgcccgt ttaattattg atggaacaga atgggccggc aatgttgaaa ttgatcctac
    25681 aagtgccgat tggtataatc acaaacatca tatcaataaa gattattcta aactaatact
    25741 gcacatcgtt tatgaattaa ataaaccatt agatcatggt tttcctgttt tggaatttaa
    25801 aaattttatg gatgaaggcg ctgtcagaac attccatcat ttacaagagt cgccattgcc
    25861 cataccgtgc agtaaacaaa tcaagcaaat cagtgatata acgttaaaca catggcttga
    25921 aagaatgata atagaacggt tagaacaaaa aactgctgct attcatgaca tgttgcaagt
    25981 aaataagttt gactgggaag agacatttta tttttttctt gctcgtaatt ttggattcaa
    26041 gaccaatgca ctgccgtttg aaatgcttgc acgttctatt cccatgactg cacttgcacg
    26101 gcataaaaat aacctattgc agattgaagc attgctgttt gggcaggccg gattcctcag
    26161 tgataaacat actgacgaat atgttgaatc tttaagacga gaatatcgtt ttcttgcagg
    26221 caagtttcag atgaagccaa tgaacaatag cttatggaag tttgcacgaa tgcatccgcg
    26281 caattttcct tctgtaagaa ttgcacagtt tgcctcactc attcataaca gcacacatct
    26341 tttttcaaaa attttggaag agaaagatgt taaatcgctt attcaacttt tcagtttcac
    26401 accttcctcc tattgggaca aacattttca ttttggaaaa gaatctgccg agaataaaaa
    26461 aacttttgga cgcgattctg ccgataatat tatcatcaac accattgctc catttttatt
    26521 tctttacggt aaaatcaagg ataatgaaaa attaaccgaa cgagcattat ctttgctcga
    26581 acatatcaaa ccggagaata atgtaatcat aagacaatgg aagagtgcag gcattacagc
    26641 taaacatgct tatcatacac aggcattaat tcagttgaag aatctctact gcagcaagaa
    26701 aaaatgtttg agttgtgcta ttggagtaaa aatacttaat aataagagta atgatcattg
    26761 acagtattaa aaattttttt gagcgacaag ctttcggtat ttgtgaatat tggggcgaac
    26821 gtctaaaaat cagatcatca gtcatcaggt tgtttttcat ttatctttct tttctgacaa
    26881 tcggttcacc ggtagttgtg tatatggtac ttggtttctg gatgaatctc cgaaaactca
    26941 ttcaccgtca gcgtggaggt gtatgggatt tgtagcaaaa cattacggaa ataaaaatta
    27001 gttttttgca gtatgataaa aatgtatatt aagccaacct gcagcacttg ccgtactgca
    27061 gtgcaactgg ctaaagcatg tgatgtatcg gtagaaatgg tagaatatct gattgataca
    27121 ccatcagaaa aagaactgtt ggagttaaca cagcagttag gagttaaacc ttttgactta
    27181 gtcagaacta aagagtcact ctataaagat aagtatgaag gtaaaaaaat ttctgataag
    27241 caatggctga agattttgca taagaatcca atattaattg agcgacctat aattactgac
    27301 ggcacgcatg cagtcatagg aaggccacca acattaattg tggactttct gaatcaaaaa
    27361 caaaaaacaa aaaagaaatc aaaatagctt gtgacagaaa atttagtttg tagaaaaaga
    27421 atgcaatctg ttatccgaaa attatttatt gcagtgctgc tgttatccgc tgttcaggtg
    27481 aatgcacagc aggacgaatt atttattccc cgtaatattc aaaaagctta tcaaaataaa
    27541 accagaaact tgcagggaga accaggaagt aagttttgga agaattatgc tgaatattat
    27601 ataagagtaa gttttgaccc aaaaacatta aaaataaaag gagagcaggt aattacttat
    27661 tacaacaact caccagacac gctaaagaag ttagtgtttc ggttagctcc aaatttttat
    27721 aaaaaaggaa acgaaagaaa taggattatc aatcctgaag atgttaccga tggtgttgac
    27781 attagccgtt gtgttattga tgctaaagaa attaatatcc atgacaaaca tgtagtgaaa
    27841 caaaagggca ctatccttga gataaaaata gatccgcttt ctgccagagg cggcaagaca
    27901 accgtagaaa tgaactggag ctatacgctg aataatggat cgcatgaacg cacaggcgtg
    27961 attgataaag gaagctattt tgttgcctac ttttttccgc gaataacagt ttatgatgac
    28021 attgatgggt gggatgactc cccttatatt ggacaacaag aatcttattt cgacaatgca
    28081 aactttaatg tatttatttc tgttccgcgc aattatatgg tttgggcaac gggcgacctt
    28141 cagaatccgt cagaggtgtt gcatgagtcg gtattaaaaa aatggacggt tgcattaagt
    28201 agtgaacgac ctgtttttgt cattgacagt ttagaccttc gtgataaaac agttacacta
    28261 caaaaaaagg taaatacatt tcagtttaag gccacaactc ccgattttgt ttttgctttg
    28321 agtgatcatt atttatggca agcacgcaat atggtggttg atagcttaac catgcaaagg
    28381 gcttttataa ctacagcatt taaccccgta cataaagatt tttttgaggt aaatgaattt
    28441 gcagctaaga caattgaaac tatgagtttt gattttccgg gtgtaactta tccctacagt
    28501 catttaacaa ttatagatgg attagaccag atggaatatc caatgatgat taatgacaat
    28561 ccaacaacaa cacgatttga tg
//
LOCUS       DAOQNP010000030        28459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_526_length_28459_cov_8.162442, whole genome shotgun sequence.
ACCESSION   DAOQNP010000030 DAOQNP010000000
VERSION     DAOQNP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28459
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_526_length_28459_cov_8.162442"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKY09_05915"
     CDS             <1..509
                     /locus_tag="PKY09_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12428.1"
                     /translation="IREIYAGSNVDPDQDIPKQDYIAQSGSNITLQADEVIYLKEGFH
                     AMSGSNVHITPTSPGGSAGPCNLPNAARMAAHIQQHQQKKNETLPGLGIYPNPTSNVV
                     KLHNRFMPLKAASVIILDVYGRIVYDQTNFNSTLHTPDFSHEPDGMYMAKVIHHGKTE
                     TLKFMVQH"
     gene            complement(514..1818)
                     /locus_tag="PKY09_05920"
     CDS             complement(514..1818)
                     /locus_tag="PKY09_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB12429.1"
                     /translation="MKKVLIITYYWPPSGGAGVQRWLKFSKYLSEFGWEPVVFTVANG
                     EFPEQDNSLLKDIPKNLEVIKVPIKEPYVIYKLLTGRKKNEKIHAGFLTEKKKKSFLQ
                     DFAVWVRGNFFIPDARMLWIKPSVKALQQWLNTNTVDAIASTGPPHTCHLIAMQLKDK
                     TGLPWLADFRDPWTNIDFYKDLKLTRWADKKHHRLEKAVVTTADAVVTVGKTMQEEFA
                     TQFSRKIECITNGYDDDDVVAAALSTDKQFSIAHVGTMVKTRNPESLWKALQQLLIEE
                     PSLKDHLQIKLVGKVDTLVNSAIENAGLLQFVYRIDYIPHDEVVRIQQQSQVLLLVVN
                     NTPNAKGVVTGKLFEYIAAKRPILCIGPTDGDAAKIIVETQSGHCAAYDDVASIKTIL
                     KDYFNKFLKSELKSDAKNIEAFCRRNLTEKLSVVLNNITQNK"
     gene            complement(1831..4248)
                     /locus_tag="PKY09_05925"
     CDS             complement(1831..4248)
                     /locus_tag="PKY09_05925"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNB12430.1"
                     /translation="MQINFKKLIPHGIAIVIFLLLTAVYFLPLFEGKGLSQHDIAQWT
                     GMSKELTDFRAKYHVEPLWTNAMFSGMPAYQISTLYPANLIQYVNDIMFFFLPSPASY
                     VFLALLSFYILMLVLKFDYRMGIAGAIAFAFSSYNFVIIAAGHNSKAHAIALMPLVFA
                     GIILTFRGRLWFGGALTAMALALQLYANHLQITYYLMIAILVMTIAYFIDAIMKKELG
                     GFIKSASVLAIAAVIGILPNYTSLAATYEYGKYSTRGPSELTSKKESSGLDKDYAFSY
                     SYGISETFTLIIPRFHGGASVSPLDESSETYKALTSNGVPVQQAKSFIQSVPLYFGNM
                     LSTSGPPYAGAIVCFLFVAGLFLIKGKEKWWLLVATILSFMLSWGENFLSFNELFFYH
                     FPGYNKFRAVSMILTIAQFTMPLLALLAMKEIFEGKTNKNDILKSLKMAFYITGGFCL
                     LFTLLPGMFNDFSSQADEQLKQYDWLITAIKQDRESAVRMDALRSLFFIAATFGLLWF
                     MLKDKIKASVAVITIAVLFIVDLWPVGKRYLNDNNFVAKSKTQKPFEPTAADTQILQD
                     TTPGFRVLNTTLNAFSDASTSYFHRSIGGYHGAKLKRYQELIENCISKNNMSVLDMLN
                     TKYLIVSDKGQQPVAQLNPGAIGAAWFVDTVKTVENADAEIKALENFNPRTIAFVDKR
                     FENTLKSFAPQHDSTATIKLNSIVCNHLVYETQTALPQFCVFSEIYYDKGWNAYIDGQ
                     KADYVRANYVLRAMAVPAGKHKIEFKFEPEVYFTGEKISLAGSIVLLLLFAAGAWIEV
                     KSGTDKK"
     gene            complement(4365..7625)
                     /locus_tag="PKY09_05930"
     CDS             complement(4365..7625)
                     /locus_tag="PKY09_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HNB12431.1"
                     /translation="MQNFIKSKLAMLIIAVFLYGQSHAGNNCWKPVEEVSLSGLGKMN
                     RNTIPSTARFYELNMQQLQSLLQQAPNRGQQSNLIIDFPDAYGKMNAFRVYASPIMPA
                     VLSSKYPMIKTYCAQGIDDPTATMRFSVTQFGLHCMTLSGTNPTNYIDTYTKDLNYYI
                     VYDKSSLANNVRNFECLTDEDAELPSMRNSVAGINNNTDDQLLRTYRLALTCTAEYGN
                     IFATTPGNEKADIMAQMTITMNRVNGVYERDLAITMEFVPNNDTLIFYGNTSQDPWSN
                     EWNTKTAQVCDQYIGVNNYDIGHNFNTTGGGNAGCIACVCLSSSQSGTHKGRGMTGSA
                     NPTGDPFDIDYVAHEMGHQYGGYHIMNTCSRSGSGNTEVEPCSGSSIMGYAGICSVNI
                     QAHSDDDFNYVNVRDISANIQNGSSTCAVTTALTNSPPTADAGADFTIPKGTAFVLEG
                     TATDADGMASLTYNWCQNDAAQAPSSSSPQPTWAVGPLSRAIKPGTSPNRYMPNLRSV
                     VLKNLYPTWEKTPDVARTLNYSFIVRDNDVNGGQTASDLMQVTVASSGPFVVTSQNTP
                     TNWNAGATETVTWNVAGTDVAPVNSPFVNIFLSLDSGFTYPYTLATNVPNNGSANVVV
                     PPGAASPWGRVMVRGAGNIFYALNGSYINIQASEFVMNMSNSTQSVCPPNDITYNFMY
                     NTFLAFNETTVFSVSGLPAGATGTFTPATAVNDSTPVQLLITGLTPSMTGTYNLTITG
                     TSPSVTKSTSVTLDILDVNLPAATLTLPADGSSGIASVATLTWQAALSPGTLFDIEVA
                     TDSLFTNVIASATGISTNTFTTNPLLSSTTYYWHVKSYNSCFANSFSASWSFTTNNCS
                     NLASANVPVVIPSSGAPTVTSTLNIASAGTITDVNVVGLTGTHTWISDLTISIKSPQN
                     TTVTLFDQICGNENDFDCNFDDAAAPGALPCPPVGGGTYKAVTPLSVFNGQQAAGNWT
                     LTITDHFNSDGGSLNGWGLEICTTTPTGIETLVVSTSMILYPNPVKELLNMNVAGQKG
                     EHIVVEIKNIAGQVIKAINVLANTKVSVDVQDLSAGMYFVTATGSHVETQRFIKAK"
     gene            complement(7777..8622)
                     /locus_tag="PKY09_05935"
     CDS             complement(7777..8622)
                     /locus_tag="PKY09_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNB12432.1"
                     /translation="MKFILCPTDFSSNATHAVAYASEMALKTNASLILLHTFETPILY
                     SDVAFVTVQYDYKVLHDAAAKKLKKFKEKFFVNKYAKLKVELILQQGLASARVMETAS
                     EKKVDMIVMGTTGTGAGERFLIGSNAGRVVKNAPCPVLLIPPRVKFNGLKKLVYATDL
                     SDDNLSKASELIPFAKEFNSELLFLYIDTTTLSKDEIDVKKITDLVRKKVKYPKQSGY
                     VCTDVSIQNGVSHFLKKFKADALVMYTRHRNLFKGLYKPSIAKSVSYNTKVPLLVVHE
                     NDTLF"
     gene            complement(8644..9063)
                     /locus_tag="PKY09_05940"
     CDS             complement(8644..9063)
                     /locus_tag="PKY09_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNB12433.1"
                     /translation="MHSIGSPEVFAVFLCRVLLGILFLFQGYEKLFTIGITKVTEIYR
                     SELVKKGIPLSVVKPLLTLSSFIEFAGGLLLIIGLFKYYSLYLLGLNMIGASLAFSLM
                     RPMWDLQHVFPRLALLVTLLLLPDAWDLISIDHFFIK"
     gene            9206..10177
                     /locus_tag="PKY09_05945"
     CDS             9206..10177
                     /locus_tag="PKY09_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNB12434.1"
                     /translation="MLSFLTERFKQHKENEEYYKLARSRYVIAHEYYRFRISVRQALV
                     SLIFVSLGIISAGFGLKGFLLPNQFIDGGVTGISLLVSLKTAISVSILIVVINIPFLI
                     LAYKQIDRRFSVKSIIAIVFLALAVYYIPYPTVTNDRLLVSIFGGFFLGMGIGFAIRG
                     GAVLDGTEVLAIYISRRSGLSIGEIIFAFNIVIFSFAAVLISIEVAMYSILIYLAASK
                     TIDFILEGVEEYTGVTIISSHSDEIRKLITEKLNMGVTIYNGKRGYGKNGHNLAEIEI
                     IYTVVTRLEISKLKTEIEHIDPNAFIIMNTVRDTKGGMIKKRTLKDF"
     gene            complement(10229..10738)
                     /locus_tag="PKY09_05950"
     CDS             complement(10229..10738)
                     /locus_tag="PKY09_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB12435.1"
                     /translation="MTKPIKFFIAGAFVCSSLFASAQIKLGHINSTQLLAMMPETKTA
                     DSTLQKFGETLQAQMKTMTAEYDSKVAEYQEKANAMAEPIKAAKEKEIRDLGDRIQDF
                     QESAQTSVQKKKEEVYSPIIKKADEAIKAVAKEKSYTYIFDSSVGALLYTTDSDDIMS
                     MVKEKLKLK"
     gene            complement(10766..11416)
                     /locus_tag="PKY09_05955"
     CDS             complement(10766..11416)
                     /locus_tag="PKY09_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNB12436.1"
                     /translation="MKKIITALALTVTSSISVMAQKYAYVNSQYILDNIPEYKSAMQQ
                     LDQISVNWQKDIEAKYAVIDKLYKDYQAEQILLTEDMKRKREAEITAKEKEVKELQKS
                     KFGYEGDLFKKKQELVKPIQDKIYNAVKKMAVDQSYAVIFDKSSDLTMLYTNPKYDKS
                     DEILNAMGYKAKVTEGGGKSGGSSSSSGSGSNTGTKDSGKSMDSPSRSDEAQPIKK"
     gene            complement(11518..14016)
                     /gene="bamA"
                     /locus_tag="PKY09_05960"
     CDS             complement(11518..14016)
                     /gene="bamA"
                     /locus_tag="PKY09_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNB12437.1"
                     /translation="MHKHILGFLTFFILLSTFSIAQVTVGDDQATLDYNNPKEYEIAA
                     ITVSGIKYLDQNALKILSGLSVGEKVKVPGEKFTKAIENLWKQGLLTDVKIVATRIEG
                     KKIFIDLQLQEKPRLSKFSFTGVRKGHADKIREKIQISKGRVLSDNALNTVNTQVLNF
                     YKEKGFLNAKVNLTTIKDTTETNSEILVINVDKGKRVKIHRINIIGNEEVSSVKLKRA
                     MKGTREQRWRYFFNSKKFDEDKYTDDKSNLISKYNEKGYRDAQILSDSVYPVSRRRIN
                     IDIKVSEGNRYYFRNISWIGNTKYTSAELNKVLGIKPGDVYNAKRLESSLYMSESSRD
                     ITSLYMDDGYLFFNIDPVEISAENDSIDLEMRIYEGKQARINHVTVKGNTKTNDRVIL
                     REIRTKPGQLFSRSDIIRTQRELSQLGYFNPEKMGVNPKPNPVDGTVDIEYEVEERPN
                     DQVELSGGWGANQVVGTLGVTFNNFSARNILNTKAWRPLPAGDGQRLSIRATTNGIYY
                     QSYNASFTEPWLGGKKPNALTVSIYHSIQTNGLKKSNDNRQAIFISGATVGLQKRLKW
                     PDDYFTLYSGINYGLYTLKNYRSTFLFTDGTSNNISIEENLTRSSVDQPIYPRKGSEI
                     SFSVQFTPPYSAFTNKDYSKLDTKEKYKWIEYHKWKFSVAWYTKLLGNLVMYNKMQFG
                     FLGFYNKGIGQSPFERFYLGGDGLSGYALDGREIIALRGYENSALTPTDNIGNDVGGT
                     IYDKFVTELRYPVSLNPSATIYVLGFFEGGNSWSTFREFAPFSLQRSSGVGLRVFLPM
                     FGLLGLDYGWRLDPQRDDPRTGVGKFSFVIGQQF"
     gene            complement(14009..14761)
                     /locus_tag="PKY09_05965"
     CDS             complement(14009..14761)
                     /locus_tag="PKY09_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNB12438.1"
                     /translation="MSIKNQIITDRLPRHVAIIMDGNGRWAKKQGKLRMFGHQHGVAA
                     VRDTVEGAAELGIEYLTLYAFSTENWNRPAIEINALMELLVNTISKETKTLMDNNIRL
                     NAIGDIESLPGRCNKNLKAAIDKTAGNSRMTLTLALSYSSRWEITRAIKNIAQDVKDG
                     KLNATEINDSLVEKYLTTAGIPDPELLIRTSGEYRVSNFLLWQIAYSEFYFTNKLWPD
                     FRREDLFESIVSYQNRDRRFGGVNESTNAKHA"
     gene            15043..17073
                     /gene="uvrB"
                     /locus_tag="PKY09_05970"
     CDS             15043..17073
                     /gene="uvrB"
                     /locus_tag="PKY09_05970"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNB12439.1"
                     /translation="MDFRINSPFNPTGDQPQAINELVKGLNRGDRHQTLLGVTGSGKT
                     FTIANVIEKVQRPTLILSHNKTLAAQLYGEFKQFFPDNAVEYFVSYYDYYQPEAFIPT
                     TNTYIEKDLAINDEIEKLRLSTTSTLLSGRKDIVVVSSVSCIYGLGNPADFLKGIIRI
                     SKGERISRNTFLHALVTALYSRTEAEFKRGTFRVKGDTVDLFLAYAERALRFTFFGDE
                     IEEIESIEPATGKRIELLDHVAIFPATIFVTNAKQQLKAIWQIQEDMVKQVDYFKENG
                     RNLEAKRLDDRVTHDLEMIRELGYCSGIENYSRYFDGREPGMRPFCLLDYFPEDYIMV
                     IDESHVTIPQIRAMYGGDRSRKVNLVEYGFRLPAAMDNRPLKFEEFETLQQQVVYVSA
                     TPADYEMTKSEGVIVEQLIRPTGLLDPLIEVKPCGNQVDDLLEEIDVTVKKNERVLVT
                     TLTKRMAEELAKYLTKLSIRCRYIHSEVETLDRVELLRDFRLGLYDVLIGINLLREGL
                     DLPEVSLVAIMDADKEGFLRSNRSLTQTAGRAARNINGRVIMYADKITESMRKTIDET
                     NRRREKQTKYNIENNIVPTPLNKSREMILQQTVVSATKNVNAYIESENVTIAADPVVQ
                     FMTEEQLANVITKTKKQMEVAAKEMDFMEAARLRDEMFELQKLMKDRFGKSV"
     gene            17213..18889
                     /gene="hutU"
                     /locus_tag="PKY09_05975"
     CDS             17213..18889
                     /gene="hutU"
                     /locus_tag="PKY09_05975"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNB12440.1"
                     /translation="MTIISDKLDISKIKTPTGNTISCKGWVQEAALRMLHNNLDPDVA
                     ERPEDLIVYGGTGKAARNVESFHLIVKALQDLNEDETLLVQSGKPVGILPTHKDAPRV
                     IIANSNLVGHWANWDYFRELEAKGLIMYGQMTAGSWIYIGSQGIVQGTYETFGEVARQ
                     HFGGSLKNTLNVTAGLGGMGGAQPLAITMNEGTCLAAEMEEWRADKRIQTRYLDEKYS
                     DLDKAIDRAVEAKQKGEAISIAYIGNAIDMLARLLERNIIPDTLTDQTSAHDALVGYF
                     PEGLNVADAKVLRESNPEEYIKQSKQSMRKHVELMIALQKKGAVTFDYGNNIRQQALE
                     MGLKNAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPNDIYETDKKILELFPENKGLH
                     RWITMAQKRIAFQGLPARICWLGQGEREKAGLAFNELVKSGKVKAPIVIGRDHLDCGS
                     VASPNRETEAMKDGSDAIADWPILNALINTAGGASWVSLHHGGGVGIGYSIHAGMVIV
                     ADGTADAATRLKRVLHNDPAMGVLRHLDAGYDIAADTARKHRLNLRDRLG"
     gene            18928..19293
                     /locus_tag="PKY09_05980"
     CDS             18928..19293
                     /locus_tag="PKY09_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12441.1"
                     /translation="MLAFTACTPDDDSTGDDRDKFTGSWTCRDSNITTGVTDPLYSVN
                     ISKVGIDDTIMISNFYNLGGSTFVKAVVSGTSVVIPNQYDDSYLINGSGIYSNSEVKL
                     NYSASLGSSMNNISAKYSR"
     gene            complement(19298..20476)
                     /locus_tag="PKY09_05985"
     CDS             complement(19298..20476)
                     /locus_tag="PKY09_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12442.1"
                     /translation="MKFRLTLLILLIFAQLNAQTGLDKSEMRDMIAICNSFTFIDLYK
                     SDKNIIPKDYHKVYTSGVFGMDNKFQIYKKNHTAVIHFRGSTDKQVSWLENIYAAMIP
                     AQGVINLEGEDFPYHFADDTSAAVHSGYSLGLAFMHAELIDRIKILNDDGIYDIIITG
                     HSQGGSLANLATAWLNNIKGKEISAKNNFRTYAFAAPMIGNQAFVNEYNRKFCDTKKS
                     FNIINIADQISSFPISYNDSASLKNSLWGMLFDNQSFNAKKAATEQGIIMLEDKLGQI
                     LRSVSESAHKRIVKNVGYAELPDYKKEFNYKPLGNRVELEAAPYPLMLRDSSILQNDS
                     LMKVYKRDVNGNFTDENLYKKAGWGWQHKPYNYYATILRMYFPADYSRMKRKYLPENL
                     "
     gene            20692..22140
                     /locus_tag="PKY09_05990"
     CDS             20692..22140
                     /locus_tag="PKY09_05990"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HNB12443.1"
                     /translation="MSKIKFIPVLLTAFILSAFQSNASSDGYQVKVKITPVFKDSLYY
                     LANYFGDKQYIQDSAKADAAGIVIFKGKEKLPGGIYLFVVPGKKYFELIIDKEQNFTM
                     ETDTADMVKNMVVKGSDDNQKFYNYLRFVMDKQKEAETLQASMHNAKTPKDSTDALDK
                     LKDLGKVVTDFKLKYITDYPDFLLSKVFKTSQEPEVPEAPLLPNGMKDSTFAYRYYKA
                     HYLDNVDFTDARILRTPLFHTKLDTYIKKLVMQVPDSINKEADMLVTKARPNPEVFKY
                     VVWYLTNTYETSNIMGMDAVFVHMAKTYYTKDQATWVDSTTLYKIQERVKVLEPILIG
                     QKVKNLIMEDTTGTYKALYNIKTPYTILLFWDPDCGHCQKVVPEVKKLYDIVKGKGAE
                     VYAICTETEMDKWRKFIREKHLDWINVADPKYQNNFRYEFDITSTPQIYLLDSNKEIV
                     AKKIDVEILADILSKKLNMKIEGIKNPEKPAH"
     gene            22144..23544
                     /locus_tag="PKY09_05995"
     CDS             22144..23544
                     /locus_tag="PKY09_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12444.1"
                     /translation="MFLQYLYARGDRYQFTGMDIFYSLFLLVFIFYFAKSIRDKHIEE
                     NPSYRYFLGGLMAKIVGGIMVVLVYTLYYSGGDTIYYFFDSMTMTNLLFKNFIHFGDV
                     VINGWENYKFSYLDSTTGTFVYYRSPESFFIARILWPFTLVGLQQMVPTVMLLALVSF
                     GGVWRMYQIFIMEFPKLERPLAYTFFFLPSVFFWGSGILKDSITLSALGYFFYSFYYV
                     FIVPKKVFKNLVIVIISAWIIISIKAYIFIGVLPGALIWIVSKLTSKIRGGFIRKVTF
                     PLIMIIVGLSGFLLLNTLSEELGKFKIDRLLDEAVVSNTDLQSDYYGGNSFSIGEIEP
                     TVQSMLIHMPLAINAALFRPYITEARNVMMMVSGLENLFILLFTLYVLYKVKIIGIFK
                     YFNKNSLLTFSLIFSLFFAFAVGISTSNFGSLVRYRIPLLPFYVSSLIIIRYLYEKEK
                     TEKRELSYSYYKAITE"
     gene            24169..>28459
                     /locus_tag="PKY09_06000"
     CDS             24169..>28459
                     /locus_tag="PKY09_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12445.1"
                     /translation="MIRHLLTTGIFFVISFSSIAQNYLMSNTNVSTCAGNFYDSGGGG
                     ANYGLNQNTTMTLCSNTAGQCIRVSFTAFNLENSFDFLRIYNGPNTASPLIGSYTGTT
                     SPGVVTGTSGCLTFVFTSDGSINHAGWTASISCVTCAAGGSCLQTCNGGPAPANDACS
                     GAQNLGMLPAPAACPNGIGSWVNVSTTNLCATAEMPYTTLTGCNPSGNMASPAADVWY
                     RLNLTGPTLNVQISGGIQTPNLALYAGTTCNNLVGRGCAIGAGGILNTSFGGLAAGTY
                     YLQVSGGNLNDQCDFNLALQNNFDCQGCVIVNNLVVNPPPVNGTYQAGQLVNFCYTIS
                     DYNQTSVNWLHAVVPTFGAGWDMSTLTTVMPANCSGAGAWGWYNTNITSSATGVIHGP
                     GFYYESGLGSPLGFMDGNPGNNFGDNNATNSCNWTFCFSVRTMPASQCTQGASLNVSI
                     DTYGDGESGSWTSLACTGDPVTDFFATLACCQPPIVNPNNPLCDGYVGSANGIAQGVS
                     PWTYIWQNSSGGTMMQVNNINSAHAIPNLAAGNYSLTTIDNNGCSSTTYFSITTPPAI
                     VIAANAIGTKCNSNDGAINIFANGGVAPLQYSIDNGTTFSAASVFNNLAAGSYTVVVK
                     DANGCTSSQVVSVAPSSTPVINSSPVTQVSCFGGNNGNIVVNAAGGNPPLQYSIDNGT
                     TYQASNSFTTLTAGTYSVVVSDVDGCTSSVTLNVTQPAQINISNVLTTDATCGNSNGS
                     LVVTASGGTGALQYSITGGVPYQASNTFNNLAAANYNVVVIDANGCTQTINAQVNNSS
                     APVINATNATSLLCNGDNNASITINASGGTGALQFSINNGTTFQSGNVFNGLVAGTYN
                     VVVSDAVGCLVSTAIVIADPPVLSISANNVGTTCTQSNGSITASGGGGSGVLQYAING
                     GSYQPSTNFNNLAAGNYTVTVQDANGCTASTTTNITDAPSAVIVNITTTPVGCNGQSD
                     GTVDIVANSGTAPLNYSIDNGLTFQAGSFFSGLAAGSYSIVVSDANGCTTTSSINVVE
                     PTTLILNLNSVNTTCSQTNGSVSAIANGGTPAYQFSIDAGVTFQASASFGGLATGVYS
                     IVVQDANGCTASGSINISDTPGPHIDNVASVDINCNGANSGSVTITASNGTTPLQFSI
                     DNGTTYQSGNVFNSLSPGSYNIVVQDANGCTTTAAANITEPTAVVISGTPVTDANCNG
                     ANNGNITINANGGTGALQYSIDNGTTYQSGNVFNSLSPGSYNIVVQDANGCTTTGTAN
                     ITEPTAVVISGTPVTDANCNGANNGSITINANGGTGTLQYSIDNGVTYQSGNVFSNLS
                     PGSYNIVVQDANGCTTTTTANITEPTAVVISGTPVTDANCNGANNGSITINANGGTGA
                     LQYSIDNGATYQTTNTFSSLSPGSYNIVVQDANGCTTTTTANITEPTAVVIS"
BASE COUNT         8567 a         5145 c         5691 g         9056 t
ORIGIN      
        1 ccattcgcga aatatatgca ggcagcaatg ttgaccccga tcaggatatt ccaaaacaag
       61 attacatagc acaaagtggc agtaacatta ccttgcaggc agatgaggta atttatctca
      121 aagagggttt tcatgccatg agtggcagca atgttcacat tacgcccaca tcgccaggag
      181 gttcggcagg gccatgcaat ttgccaaatg ctgcacgtat ggctgctcac atccaacagc
      241 atcaacaaaa gaaaaatgag accctacccg gtcttggtat ttatcccaac cctacttcaa
      301 atgttgttaa gctccataac cgttttatgc cgctaaaggc agcaagtgta ataatactgg
      361 atgtttatgg gcgaattgtt tatgatcaga caaactttaa cagcacatta catacaccgg
      421 atttcagtca tgagcctgat ggcatgtata tggcaaaggt aatacaccac ggaaaaacag
      481 aaacactgaa atttatggtg cagcattgaa atattatttg ttttgagtaa tgttgtttaa
      541 caccacagac agtttctctg ttaaatttct acggcaaaaa gcttcaatgt tttttgcatc
      601 ggatttaagt tcgcttttca aaaatttatt gaaataatct ttgagtatag tttttatact
      661 ggcgacatca tcataagctg cacaatgtcc actttgtgtt tcaacaataa tttttgctgc
      721 atcgccatct gtggggccaa tgcacaatat aggtcgttta gcagctatat attcaaaaag
      781 ttttccggtc accacacctt tggcattggg agtgttgttg accacaagta gtagtacttg
      841 tgattgttgt tgtatgcgca ctacctcatc atgaggaatg tagtctatgc gataaacaaa
      901 ttgcagtaag ccggcatttt ctattgcgct atttactaaa gtatctactt taccgactaa
      961 ctttatctga aggtgatcct ttaaagaagg ctcttctatt aaaagctgtt gtaaggcttt
     1021 ccataagctt tcagggtttc gtgtttttac catagtgcct acatgggcaa tgctgaattg
     1081 tttatctgtt gacagtgcag cagctacaac atcatcatca tcgtagccgt tggtaataca
     1141 ttcaatcttt ctgctaaact gagttgcaaa ctcctcttgc atggtctttc ctacggtgac
     1201 aacggcatcg gctgtagtta ctacagcttt ttccaggcgg tgatgttttt tgtctgccca
     1261 gcgtgttagt tttaaatctt tgtaaaaatc aatatttgtc catgggtcac ggaagtctgc
     1321 taaccaaggc aatcccgttt tgtcttttag ctgcatggct atcagatggc aggtgtgggg
     1381 agggcctgtg ctggcaatgg catcaacggt attggtattt aaccactgct gcaatgcctt
     1441 caccgaaggt ttgatccata acatccttgc atctggaata aaaaagttgc ctcgcaccca
     1501 cacggcaaaa tcctgaagaa agcttttctt tttcttttct gtcagaaatc ctgcatgaat
     1561 cttctcgttt tttttacgac ctgtcagcag tttataaatt acataaggct ctttgatggg
     1621 tactttaata acttccagat tttttggaat gtcttttaac aaggaattgt cttgttccgg
     1681 aaattcgcca ttagcaacgg taaacactac aggctcccaa ccaaattcac taaggtattt
     1741 tgagaatttt aaccatcgct gcacacctgc accaccacta ggtggccagt aataagtgat
     1801 gattaatact tttttcaagg caatgaactg ttatttctta tcggttcctg attttacttc
     1861 aatccatgca cctgctgcaa agagcaacag aagaacaatt gaccctgcaa gtgaaatttt
     1921 ttctcctgta aagtaaactt caggttcaaa cttaaattct attttatgct ttcctgccgg
     1981 tactgccatg gcgcgcaaca catagtttgc ccgcacatag tcagcttttt gcccgtcaat
     2041 gtaagcattc catcctttat cgtaataaat ttctgaaaac acacagaact gcggcaatgc
     2101 cgtctgcgtt tcataaacca agtgattgca tacaatagag tttagtttaa tggtagccgt
     2161 agaatcatgt tgaggtgcaa aagattttaa agtattttca aatcgcttgt caacgaaggc
     2221 tatagttcgc ggattgaaat tttctagggc ttttatttct gcatcggcat tttcaacagt
     2281 ttttaccgta tcaacaaacc aggcagcacc aattgcaccc ggattaagtt gtgccacagg
     2341 ctgctgtcct ttatcactta caataagata ttttgtgttg agcatgtcaa gcacactcat
     2401 gttattttta gaaatacagt tttcaatcag ttcctgatag cgctttaatt tggcaccatg
     2461 atatccgcca atacttcggt gaaaatagga ggttgaagca tcgctgaaag catttaatgt
     2521 ggtgttgagt acacggaatc cgggtgtagt atcctgcaaa atttgtgtat ctgcagcagt
     2581 gggttcaaaa ggtttttgtg ttttactttt tgccacaaaa ttgttgtcat tgaggtaacg
     2641 ttttccaaca ggccataagt caacaataaa aagaactgca atagttatta cggcaacact
     2701 tgcctttatt ttgtctttta acataaacca tagtaatccg aaagttgcag caataaaaaa
     2761 taacgagcgt aatgcatcca ttctcactgc actttcgcgg tcttgtttta tggcagtaat
     2821 cagccagtca tactgtttaa gttgttcatc ggcttgcgag ctaaaatcat taaacattcc
     2881 tggtagtaat gtaaacaaca aacaaaatcc acctgtaata taaaatgcca tcttaagtga
     2941 tttcaatata tcattcttgt tggttttacc ttcaaatatt tctttcattg caagaagagc
     3001 gagtagtggc atcgtgaatt gcgcaatggt gagtatcata ctcacagccc tgaatttatt
     3061 atatcccgga aaatgataaa agaagagctc attgaatgag agaaaatttt caccccatga
     3121 aagcataaat gaaagaattg ttgctaccag cagccaccat ttctctttgc ctttaattaa
     3181 aaacagacct gcaacaaata gaaaacaaac tattgctccg gcatagggag gacctgaggt
     3241 ggagagcata ttaccaaaat aaagtggaac agactgtata aagctttttg cttgttgtac
     3301 aggcactcca ttacttgtta atgccttata agtttctgaa ctttcatcca acggactaac
     3361 agaagcgcca ccatgaaagc gtggaatgat gagtgtaaat gtttcgctga tgccataact
     3421 gtagctgaat gcataatctt tgtcaagacc tgaagattct tttttagagg taagttcgct
     3481 tggtccccgg gtggaatatt ttccatactc atacgtggct gccagactgg tgtaattggg
     3541 tagtattcct ataactgcgg caatggccag tacggaggca ctttttataa agccgcctaa
     3601 ctcttttttc atgatggcat caatgaagta agcaatggtc attactaaaa ttgcaatcat
     3661 cagatagtaa gttatctgaa ggtggttggc atagagttgt aaggcaagtg ccatagctgt
     3721 taaagcacca ccaaaccaaa gccttccccg aaaagtgagt ataatacctg caaataccaa
     3781 tggcataaga gcaatggcat gtgcttttga attgtgtcct gctgcaatga ttacaaaatt
     3841 ataactagaa aaagcaaaag ctatggctcc ggcaattccc attctataat caaatttaag
     3901 taccagcata agaatgtaaa aacttaataa agcgagaaac acataggatg ccggagaagg
     3961 taaaaagaaa aacataatgt cattgacata ttgtatcaga ttagcaggat acaaggttga
     4021 aatctgatag gcaggcattc cactgaacat ggcatttgtc cataatggtt caacatgata
     4081 ttttgcacgg aagtcagtaa gttctttact catgcctgtc cattgagcga tatcatgttg
     4141 actcagtccc ttaccttcaa atagaggtaa aaaatatacg gctgttaaga gtaaaaaaat
     4201 tacaatggct atgccgtgag ggattaactt cttgaagttt atctgcatac gatttcttta
     4261 ttgggcaaat gtaatagttt ggaaaggaat aatcctgttg tttttcaggc aatagaaaag
     4321 ccttaacatc ggggatatta aggcttttag cttatatggg aaaattactt tgctttaatg
     4381 aagcgctgtg tttctacatg acttcctgtg gctgtaacaa aatacatgcc tgctgaaagg
     4441 tcttgaacat caacagaaac ttttgtattt gcaagaacat tgatagcttt tatcacctgt
     4501 cccgcaatat ttttaatctc taccacaata tgctctcctt tttgacctgc aacattcatg
     4561 tttaataatt cttttacagg attaggatat aaaatcatag aagtagaaac aacaagggtt
     4621 tcaatacctg ttggtgtggt agtgcagatt tcaagccccc agccattcaa tgagccacca
     4681 tcagaattaa aatggtcagt tattgttaat gtccagttgc ctgctgcttg ttgcccattg
     4741 aatacactca gcggtgtaac ggctttataa gtgccaccac ctacgggagg acaaggaagc
     4801 gcaccgggtg ctgcggcatc atcaaaatta caatcaaagt cattttcatt accacaaatc
     4861 tgatcaaaca atgttaccgt tgtgttttgt ggacttttaa tacttattgt aaggtcacta
     4921 atccatgtgt gcgtaccggt aagaccaact acattgacat ccgtaatagt tcctgctgaa
     4981 gcaatgttta atgttgaagt aactgtagga gcaccacttg acggaataac aacaggaaca
     5041 tttgcacttg ctaaattcga gcaattgttg gttgtaaatg accatgaagc gctgaagcta
     5101 tttgcaaagc atgaattata cgacttcaca tgccaatagt aggttgtact tgatagcaac
     5161 gggttagtag taaaagtgtt tgtgctgatg ccggttgcag aagcaattac attcgtaaat
     5221 aatgaatctg ttgcaacttc aatatcaaat agtgttcccg ggcttaaagc agcttgccag
     5281 gttaaagttg caacggaagc gatgccactg ctgccatcag caggtagtgt taatgttgcg
     5341 gcaggtaagt ttacgtctaa aatatcaagt gtaacagaag ttgactttgt tacagatgga
     5401 gaagttccgg tgatagtaag attatatgtt ccggtcatag aaggtgttag tcctgtaatc
     5461 agcaattgca caggagtgct gtcatttact gctgtggcag gagtaaatgt tcctgttgca
     5521 ccggcaggaa gtccggatac agaaaataca gttgtttcat tgaatgccag aaaagtgttg
     5581 tacataaaat tataagtaat gtcatttgga ggacaaacac tttgtgttga attactcata
     5641 ttcattacaa attccgatgc ctgaatgttg atataacttc cgtttagcgc ataaaaaata
     5701 ttgcctgcac ctcgaaccat cacacgtccc catggtgaag cagcacccgg tggtactaca
     5761 acattggcac tgccgttatt aggtacgttg gttgcaagtg tatatgggta agtgaatccg
     5821 ctgtcgagag aaagaaaaat atttacgaat ggtgaattaa ccggagcaac atctgtgcct
     5881 gctacattcc atgttacggt ttcagtagca cctgcattcc agtttgtagg tgtgttttgt
     5941 gaagtaacaa caaatggacc tgaagaagct acggttactt gcataaggtc tgatgctgtt
     6001 tgaccaccat tcacatcgtt atctctaaca ataaaagagt agtttaaagt tctggctaca
     6061 tcaggcgttt tttcccatgt aggatataag ttttttagta caacacttct caggttgggc
     6121 atataacggt taggagatgt tccgggtttt attgctcgtg acaagggtcc aacagcccat
     6181 gttggctgcg gactgctgct tgaaggggct tgtgctgcat cattctgaca ccagttatac
     6241 gttaacgaag ccatgccatc agcatcggtt gcagtacctt ctaatacaaa tgctgttcct
     6301 tttggtatgg taaaatcagc gcctgcatca gcagtgggag gactgtttgt taatgctgtt
     6361 gtaactgcgc aagtgctgct accgttttga atgtttgcag aaatatctct tacgttaaca
     6421 tagttaaaat catcatcgct gtgtgcctgt atgtttacag aacaaatacc tgcatagccc
     6481 ataatggaac tgccactgca aggttctacc tctgtgtttc cactaccact acggctacaa
     6541 gtattcataa tgtgatagcc accatactga tgtcccattt catgcgcaac atagtcaatg
     6601 tcaaaaggat caccggtggg gttggcactt cctgtcatac cacgaccttt atgtgtgccg
     6661 ctttgcgagg agcttaaaca aacacaggca atacatcctg catttcctcc acctgtagta
     6721 ttaaagttat gtcctatatc gtaattgttc acaccaatat attgatcaca aacttgtgct
     6781 gtctttgtat tccactcatt tgaccaagga tcctgagagg tgtttccata aaaaattaac
     6841 gtgtcattgt tgggaacaaa ttccatagta attgcaaggt cgcgttcgta aacaccattt
     6901 acacgattca tggttattgt catttgtgcc atgatgtcgg ccttttcatt accgggagtt
     6961 gtggcaaaaa tatttccata ttcagcagtg caggtaagtg caagtctgta tgttctcaat
     7021 aattgatcat cagtattgtt gttgatacct gcaacgctat ttctcatgct tggcagttca
     7081 gcatcttcat cagtgagaca ttcaaaattg cggacgttgt tggctaaaga agatttatca
     7141 taaacaatat agtaattcaa atccttggta taagtatcaa tataatttgt aggatttgtt
     7201 cctgacaaag tcatgcaatg cagaccaaac tgtgtaacgc tgaaacgcat tgttgcagtt
     7261 gggtcatcaa tgccttgtgc gcaatatgtt ttaatcatcg gatacttgga tgaaagcact
     7321 gccggcatta taggtgatgc ataaactctg aaagcattca ttttgccgta agcatcagga
     7381 aaatcaatta tcagatttga ttgctgccca cgattaggtg cctgctgtaa taaactttgc
     7441 aattgctgca tgttcaattc ataaaaacgc gcagttgacg gaatggtgtt tcgattcatt
     7501 tttccgagtc ctgataaaga aacctcttca acaggtttcc agcagttatt tcctgcatgg
     7561 gattgcccat agagaaatac tgcaatgatt agcatggcta atttgctctt aataaagttt
     7621 tgcatgtgta atttaatttg tttttgtttg atttggaaat gatgtatgag ttatacctca
     7681 catagcaatt atttcgctaa tatacatgga acaaaaacaa gtttaaccac agtttgcagt
     7741 cagttaataa atgagtaatc tttgcgcgtt ttaaagttaa aaaagggtgt cgttctcatg
     7801 tactaccaat aatggaacct ttgtgttgta agacacactt tttgctatac ttggcttata
     7861 caaccctttg aacaagttac gatgacgggt gtacatcacc aaagcgtcag ctttgaactt
     7921 tttaaggaaa tgactcacgc cattctgaat agaaacatcg gtacagacgt atcctgactg
     7981 ttttggatat tttacttttt tacgaacaag atctgttatt tttttaacat ctatctcatc
     8041 tttcgataat gttgttgtgt caatataaag aaataaaagt tcgctgttaa attctttcgc
     8101 aaatggaatc agttctgatg ctttgctcag gttgtcatct gaaaggtcag ttgcataaac
     8161 aagttttttt agtccgttga actttacacg tggtggaata agaagtacag gacaaggtgc
     8221 atttttcaca acacgacctg cattgctgcc aattaaaaat cgttcgcctg caccggtgcc
     8281 tgttgtaccc atgacaatca tgtcaacttt cttttcagag gcagtctcca ttacccgtgc
     8341 agaagctaaa ccttgttgca gtatcaactc tactttgagt ttagcatatt tatttacaaa
     8401 aaatttctct ttgaatttct ttagtttttt tgctgcagca tcatgcagta ctttgtaatc
     8461 atattgtact gtcacaaaag ctacatcact gtataaaatc ggtgtttcga aagtatgcag
     8521 aagtattaaa ctagcatttg ttttcagagc catttcgctg gcataggcaa cagcatgagt
     8581 tgcattcgaa gaaaagtcag tcggacaaag aataaatttc atattaatat tatttaagtt
     8641 atgttatttt ataaagaagt gatctataga gattaaatcc catgcatcag gtaataacag
     8701 taaggtaact aataaagcca atcgggggaa aacatgttgt aaatcccaca tgggacgcat
     8761 gaggctgaat gcaagagatg caccaatcat attcaaacca agaaggtata aagaataata
     8821 tttaaagagt ccgataatta ataataaacc tcctgcaaat tctataaatg atgataatgt
     8881 caatagcggt tttacaacag atagaggaat gccttttttt actaattccg aacggtaaat
     8941 ttcagttact ttagttatgc caatagtaaa aagcttttca taaccctgga acaaaaacaa
     9001 gatgccaagc aaaactctgc aaagaaatac agcaaaaacc tcaggtgacc caatactgtg
     9061 catgactaat ttttctacaa aattagaaat taaaaattat taacgaatga tttatctcac
     9121 aggatattgt gatattagaa attctttgaa gaaggatttt gtaaaaggaa gcagaaaaat
     9181 tttaaatttg caactataaa catctatgct ttcattctta acagagcgtt ttaaacagca
     9241 taaagagaac gaagaatatt acaaattggc tcgttcgcgc tatgttattg cacatgagta
     9301 ttaccgattc agaattagtg tacggcaggc acttgtttct ttaatctttg tttcgttagg
     9361 tattatttca gcgggatttg gattgaaggg gtttttattg cctaatcagt ttattgatgg
     9421 gggagtgaca ggcatttcgc tcttagtgag tttaaagaca gcaattagtg tttcaatatt
     9481 gattgtagtc attaatattc catttctgat tcttgcttat aaacaaattg accgcagatt
     9541 ttctgttaag agtattattg ctattgtttt tctggcatta gctgtatatt atatccctta
     9601 tccgactgta acgaatgata gattgctggt atctattttt ggcggttttt ttcttggcat
     9661 gggtattggc tttgccattc gtggtggtgc agtgcttgat ggtacagaag tgcttgccat
     9721 ttatatcagc cgaagatcag gactgtctat tggtgaaatt atttttgcat ttaatattgt
     9781 aattttcagt tttgccgcag tgctgatttc tattgaagtt gccatgtatt cgattttgat
     9841 ttatctggca gcatcaaaga ccattgattt tattcttgaa ggcgtagagg aatataccgg
     9901 tgtgacaatc atttcatcac acagtgatga aataagaaag ttgataactg aaaagttaaa
     9961 catgggtgta acgatatata acggtaagcg cggctacgga aagaacggtc acaatctggc
    10021 agaaattgaa attatttaca ctgttgtaac acgcttggaa atttcaaaac ttaaaaccga
    10081 aattgaacat attgacccca atgcgtttat tataatgaat acagtgcgcg atactaaagg
    10141 cggtatgatc aagaagagaa cactcaaaga cttctaaata aaaaaagccg tccatagaga
    10201 cagctttttt tggttttaaa tcaatctgtt atttcaactt caatttctct tttaccatgc
    10261 tcataatatc gtcagagtcg gttgtgtaaa gtaatgcacc aacacttgag tcgaatatat
    10321 aggtgtagct tttttcttta gctacggctt tgatagcttc gtcagctttt ttgatgattg
    10381 gagaatagac ttcttctttt ttcttttgaa cagacgtttg tgccgactcc tggaagtcct
    10441 gaatacggtc gcctaaatcg cgtatttctt tttctttggc tgccttaata ggctctgcca
    10501 ttgcgttggc tttctcctga tattcggcaa cttttgaatc gtattcggca gtcattgttt
    10561 tcatctgagc ctgtaatgtc tcaccaaact tttgcaaagt agaatctgca gtttttgttt
    10621 cgggcatcat tgccagcaat tgtgtggagt tgatgtgtcc taatttaatt tgagctgatg
    10681 cgaataaact gctgcataca aatgctccgg caataaaaaa tttaattggt tttgtcatga
    10741 ttaatttatt ggatgatata atttgttatt tttttatggg ttgtgcctcg tcagaacgtg
    10801 atggagagtc catgcttttg ccactgtctt tggtgcctgt gttgctgcca ctgcctgatg
    10861 agctggatga tcctcctgat tttccaccac cttcagttac tttggctttg tagcccatag
    10921 catttaaaat ttcatcactt ttatcgtact ttggattggt gtaaagcatg gtaaggtcac
    10981 tcgatttgtc gaagattacg gcatagcttt gatctactgc cattttcttt accgcattat
    11041 aaattttatc ctgtatgggc tttactaact cttgtttttt cttaaataaa tcgccttcat
    11101 atccgaactt cgatttctga agttctttta cttctttttc tttagcagtg atttctgctt
    11161 cgcgcttgcg cttcatgtct tctgtaagta atatctgctc tgcctgatag tctttataca
    11221 gtttgtcaat gacagcatat tttgcttcaa tgtctttttg ccagtttaca gatatctgat
    11281 ccaattgttg catggctgat ttgtactcag gaatattgtc taaaatatac tgagagttaa
    11341 cgtatgcata cttctgagcc attactgaaa tggaagaggt aactgtcagt gctaatgcgg
    11401 tgatgatctt tttcatttta cgtttaatta atgtgtgcaa agatatgatt ttagcttaac
    11461 catatactaa atatcgtacc actaacacca attgcggtga acaactgatg aataggatta
    11521 aaactgctgt ccgataacaa atgagaactt gcccacacct gtgcgtgggt cgtcacgttg
    11581 agggtcaaga cgccatccgt agtcaaggcc gagtaatcca aacattggaa gaaatactcg
    11641 aaggcctaca cctgatgaac gttgtaaact aaaaggggca aactctctga atgttgacca
    11701 tgaattgccg ccttcaaaga atcccaatac atatattgtt gcacttgggt taagcgatac
    11761 aggatagcgc aattcagtta cgaatttatc gtaaatagta ccacctacat cgttgccgat
    11821 gttgtctgtt ggtgtcagtg cgctgttttc ataaccacgc aatgcaataa tttcacgacc
    11881 atcaagagca tagcctgaaa gtccatcacc accaagataa aaacgttcaa atggcgactg
    11941 cccgattcct ttgttataaa aaccaaggaa gccaaactgc attttattat acatgaccag
    12001 attgccaaga agtttggtgt accaggctac actgaatttc cacttgtgat attcaatcca
    12061 cttgtatttt tcttttgtat ctaattttga ataatccttg tttgtaaatg ccgaataggg
    12121 tggtgtaaac tgtacagaaa aagaaatttc tgaacctttg cgtggataga taggctgatc
    12181 tacactgctg cgtgtaagat tttcttcaat agaaatattg ttcgaagttc catcggtaaa
    12241 taaaaacgtg gagcgataat ttttaagcgt ataaagtcca taatttattc ctgagtagag
    12301 tgtaaaataa tcatcaggcc acttaagtcg cttttgtaag ccaacagttg cacctgaaat
    12361 aaatattgcc tgtctgttgt cgttagattt ttttagacca ttggtttgaa tggagtgata
    12421 tattgaaact gtgagtgcat ttggtttttt gccaccaagc cagggttctg taaacgaagc
    12481 attataagac tgatagtaaa ttccgtttgt tgtagcacga atacttaatc tttgtccgtc
    12541 accggcagga agtggccgcc atgccttagt atttaaaata tttctcgcag aaaaattatt
    12601 gaacgttaca cctaatgttc caaccacctg attggctccc catcctccgg aaagttctac
    12661 ctgatcgtta ggcctttctt ctacttcata ttcaatatca acagtgccat caacaggatt
    12721 gggctttggg tttactccca ttttttccgg attaaaataa cctaactgcg acaattcgcg
    12781 ctgcgttctg atgatgtcac ttctgctgaa aagctgaccg ggttttgtac gaatctcacg
    12841 taaaattact ctgtcgttgg tctttgtatt tcctttaacg gtaacatgat ttatccttgc
    12901 ctgcttacct tcgtaaatac gcatttccaa atctattgaa tcgttttctg cagatatttc
    12961 aacgggatca atattgaaaa acaggtatcc gtcatccata tagagcgaag taatgtcgcg
    13021 gcttgattca ctcatataga gtgaactttc aagtcgtttt gcgttatata catcaccggg
    13081 ttttatgcct aaaactttat tcagttcggc agaggtgtat ttagtgtttc caatccaact
    13141 gatatttctg aagtaataac gattgccttc actaacttta atgtcaatgt tgattctcct
    13201 gcgactgaca ggataaacac tgtcgcttaa aatctgtgca tcacgatatc ctttctcatt
    13261 gtattttgaa atcaggttgc ttttgtcatc agtatattta tcctcatcaa attttttgga
    13321 gttgaaaaag tatcgccagc gttgctcgcg tgtgcctttc atggctcgtt ttaatttaac
    13381 gctgcttact tcttcattgc ctatgatgtt tattctgtga atttttacac gcttgccttt
    13441 gtcaacattg ataactaaaa tttcgctgtt ggtttctgtg gtgtctttga ttgtagtcag
    13501 attgacttta gcatttaaaa atcctttttc tttataaaaa ttcagcactt gtgtatttac
    13561 agtgttaaga gcattatctg atagtactct tccttttgat atctggattt tttcgcgaat
    13621 cttatcagca tgacctttac gcacaccggt gaatgaaaat ttactcagtc gcggcttttc
    13681 ctgaagttga aggtcaataa atattttttt gccttcaatt cttgtagcaa caatttttac
    13741 atcggttagc aagccttgtt tccaaaggtt ttctatggcc ttggtaaatt tttctcccgg
    13801 cacttttact ttttctccaa cgcttagacc ggataaaatt ttcaatgcat tctgatcaag
    13861 atattttatt ccactgacag taatagcagc tatttcatac tctttaggat tgttatagtc
    13921 taatgttgcc tgatcgtcac caacagttac ttgcgcaatg gaaaaagttg aaagcagaat
    13981 aaaaaaggtt agaaaaccta aaatgtgttt atgcatgttt ggcatttgtt gattcgttta
    14041 cgccaccaaa ccttctgtcg cggttttgat aactaacgat ggattcaaaa agatcttcgc
    14101 gcctgaagtc aggccaaagt ttgttggtga aataaaattc gctgtaggca atctgccaca
    14161 ataaaaaatt tgaaactctg tattcgccac ttgttctgat gagcagttcc ggatcgggaa
    14221 ttccggccgt ggttaaatac ttttctacta atgagtcgtt gatttctgtt gcattaagtt
    14281 tgccatcttt aacgtcctga gcaatgtttt ttatggctcg tgtaatttcc caacgggaac
    14341 tgtagcttaa tgccaaggta agtgtcattc tgctgttacc tgcagttttg tcaatagcag
    14401 ctttaagatt tttgttgcat cgcccgggca ggctttcaat atctccaatg gcattcagtc
    14461 tgatgttatt atccatcagc gttttggttt cttttgaaat ggtgtttacc aacaattcca
    14521 tcagcgcatt gatttcaatg gcaggcctat tccagttttc ggtgctgaaa gcataaagag
    14581 taaggtattc aatgcccagt tctgcagcac cttccacagt gtcgcgaacg gcagccacac
    14641 catgctgatg cccaaacatt ctgagcttgc cctgcttttt tgcccaacgt ccgttaccat
    14701 ccataatgat ggctacatgt cggggcaatc tgtctgtgat gatctgattt tttatactca
    14761 ttcgtcaaaa cgaaggcgca aatttagaat tttaaccgaa tgttgaagtt ttccccttta
    14821 acgcattttg aacgatttta tgtcgtttca caaaacatta atactaacga cagtttttta
    14881 acatttattt acatttgaaa agggcagtct tttagtcgaa atttcttgcc gggaatgaag
    14941 cggactgtgt tgtttatgaa agcaggttaa agatttttcc ttctctaaaa aatgtacgct
    15001 ccgtttttgg tttactagcc ttaaattctg catctttgta gaatggattt tcgcatcaac
    15061 tcacctttta accctaccgg tgatcagcct caggctataa acgagttggt gaagggcttg
    15121 aacagaggcg acagacacca aacactgttg ggtgtaacag gaagcggaaa aactttcact
    15181 attgccaacg taattgagaa ggtgcaacgc cctactttaa ttttaagtca caataagaca
    15241 ctagcagcac agctatatgg cgagtttaaa cagttttttc ctgacaatgc cgttgaatat
    15301 tttgtttcat actacgatta ttatcaaccc gaagcattta ttccaactac caatacatac
    15361 attgaaaagg accttgccat taatgatgaa attgaaaagc ttcgtttaag tacaacgtca
    15421 accttacttt ccggacgaaa agatattgtc gtagtaagca gtgtgagttg tatttacgga
    15481 ttaggaaatc ctgctgattt tttaaagggc attatccgaa tttcaaaggg tgaacgcatc
    15541 agtcgcaata cttttttaca tgcattggta actgcattgt attcccgcac cgaggcagaa
    15601 tttaagcgtg gtactttccg ggtaaaaggt gatacggttg atttattttt agcttatgct
    15661 gaacgtgcac tacggtttac tttttttggt gatgaaatag aagaaattga aagtattgag
    15721 cctgcaacag gaaagcggat tgaattgttg gatcatgttg caatttttcc ggcaaccatt
    15781 tttgttacca atgcaaagca gcaactgaaa gcaatctggc agattcagga agatatggta
    15841 aaacaggtgg actattttaa agaaaatggc cgtaatctgg aagccaaacg cctcgatgac
    15901 agagtaacac atgaccttga aatgattcgt gaattgggtt attgttctgg aattgaaaat
    15961 tattcgcgct attttgatgg tcgagaaccg ggcatgcgcc ctttctgttt gcttgattat
    16021 tttcctgaag attatatcat ggttattgat gaaagccacg taaccatacc tcagataaga
    16081 gccatgtatg gtggcgaccg ctcaaggaaa gttaatctgg ttgaatacgg tttcagactt
    16141 cctgctgcaa tggacaacag gccactgaag tttgaagagt ttgaaacact gcaacaacag
    16201 gttgtttatg ttagtgcaac accggcagac tatgagatga caaaatccga aggtgtaatt
    16261 gtagaacaat tgattcgacc aacaggattg cttgaccctt tgattgaagt aaaaccttgc
    16321 ggcaatcagg tagatgattt attagaagaa atagatgtaa cagtaaagaa gaatgagcgc
    16381 gtgttagtga caacattaac caaacggatg gcagaagagt tagctaaata tttaaccaag
    16441 ctttcaatcc gatgccgcta cattcacagt gaagttgaaa cgcttgacag ggtagagttg
    16501 ctacgcgatt ttcgtttggg attgtatgat gttttaattg gtataaatct actgcgcgaa
    16561 ggtctcgacc tacctgaggt ttctttagtg gctattatgg atgctgacaa agaaggtttt
    16621 ttgcgaagca accgttcatt gacacagaca gcaggacgtg ctgcccgtaa tattaacgga
    16681 cgtgtgatta tgtatgccga taagattaca gaaagcatga gaaagaccat tgatgaaacc
    16741 aaccgcaggc gcgaaaaaca gactaagtac aatattgaaa ataatatcgt tcctacacca
    16801 ttgaataaat cgcgcgaaat gattttgcaa cagacagttg tttccgcaac aaaaaatgtt
    16861 aatgcttata ttgaaagtga aaatgtaact atagcagccg accctgttgt acagtttatg
    16921 acagaagagc agttggcgaa tgtcatcacc aaaacaaaga aacaaatgga agttgcggca
    16981 aaagagatgg attttatgga agctgcacgt ctgcgtgatg aaatgtttga attacaaaag
    17041 ttgatgaagg atcgttttgg caagtcggtg taattgtttt ttggtttcta aactcattga
    17101 tgcatctaca gagaaaaaat taaagcactt cttcatgaca gaatcataga taaaagtgtt
    17161 ttaaaaatgc cttttaaccg ttatctttgt gcatctttaa gtatataaaa tcatgacaat
    17221 aatttcagat aaattagata tcagtaaaat taaaacgcct acaggaaaca ctattagttg
    17281 taaaggctgg gttcaggaag cagcattgcg catgttgcac aacaatcttg atcccgatgt
    17341 ggctgaacgt cctgaagatt taattgttta tggcggtacc ggcaaggctg cacgaaatgt
    17401 agagtcattt catctcattg taaaagcatt gcaagacttg aatgaagacg aaactttatt
    17461 ggtgcaatca ggcaaaccgg tgggaatttt accaacacat aaagatgcac cacgggttat
    17521 tattgccaac tctaaccttg ttggtcattg ggctaactgg gattattttc gtgagttgga
    17581 agcaaaaggt ctaattatgt acgggcagat gacagcaggt agttggattt acatcggctc
    17641 tcagggaatt gttcagggta cttatgaaac gtttggcgaa gtggcacgtc agcattttgg
    17701 tggttcactt aaaaatacat taaatgttac tgccggactt ggtggaatgg gaggtgcaca
    17761 gccattggca attacaatga atgaaggtac atgtcttgca gcagaaatgg aagaatggcg
    17821 tgctgacaaa agaatacaaa cacgctatct tgatgaaaaa tattctgatt tggataaagc
    17881 tattgaccgt gctgttgaag caaaacaaaa aggtgaagca atttctattg cctacatagg
    17941 taatgccatt gatatgcttg cgcgattgtt ggaaaggaat atcatcccgg ataccttaac
    18001 agatcagaca tcggcacatg atgctttggt tggatatttc cctgaaggat taaatgttgc
    18061 tgatgcaaaa gttttgcgtg aatctaatcc tgaagaatat attaaacagt ctaaacaaag
    18121 catgcgaaag catgtagaac tgatgattgc cttacagaaa aaaggtgcag taacgtttga
    18181 ttatggaaac aacattcgtc agcaggcttt ggagatgggt ttaaaaaatg catttgattt
    18241 tcctggattt gtccctgcat acatacgacc gttattttgc gaaggcaagg gtccgttccg
    18301 ttgggctgca ctcagtggtg accccaatga tatttatgaa accgataaaa aaattctgga
    18361 attgtttcct gaaaataagg gtctgcaccg ctggataacc atggcacaaa agcgtattgc
    18421 ttttcaggga ttgcctgcac gtatctgttg gcttggacaa ggtgagcgcg aaaaagcagg
    18481 tcttgcattt aacgaattgg ttaaatctgg aaaagtaaaa gcgcccatag ttatcggtcg
    18541 cgaccatttg gactgtggaa gtgttgcttc tccaaaccgg gaaacagaag caatgaaaga
    18601 tggtagtgat gccattgccg attggccgat actaaatgca ttgataaata ctgcaggagg
    18661 tgcaagctgg gttagtctgc atcatggtgg tggagtaggt ataggttata gtattcatgc
    18721 aggaatggta attgttgctg atggtactgc tgatgcagca acaagattaa aacgtgtttt
    18781 acacaacgac cctgctatgg gagttctgcg tcatcttgat gcaggttatg atattgcagc
    18841 agatactgca agaaagcatc gtttgaactt gcgtgataga ttgggatgag aaaaaataag
    18901 caagtattat ttttaataac gttggtgatg ttagcattca cagcctgcac acccgatgat
    18961 gatagcacag gtgatgacag agataaattt accggaagtt ggacctgtcg tgattctaat
    19021 ataacaaccg gtgtaacgga tcctctttat tcagtcaaca tttcaaaagt tggtattgat
    19081 gatacgataa tgatttcaaa cttctacaat cttggcggat ctacttttgt aaaagcagtt
    19141 gtcagcggaa caagtgttgt aattcctaat cagtatgatg acagttattt aataaatggt
    19201 tcaggaattt acagcaacag tgaagtgaag ctcaattact cagcttcttt aggcagtagt
    19261 atgaataata tttctgcaaa gtattcgaga taatttatta aaggttttca ggtaaatatt
    19321 ttcttttcat tcgtgaataa tctgcaggga aatacatcct taaaatggtt gcataataat
    19381 tatatggttt gtgttgccat ccccatccgg cttttttgta aagattttcg tcagtaaaat
    19441 taccatttac atcacgcttg taaactttca tgagcgaatc gttttgcagt attgaagagt
    19501 cacgaagcat aagcggataa ggtgctgctt ctaattctac tctgtttcca agaggtttgt
    19561 agttaaactc ctttttgtaa tcaggcaatt cggcatagcc aacatttttt acaattcttt
    19621 tgtgtgcaga ctcagaaaca cttcttaata tctgacctaa tttatcttca agcataataa
    19681 ttccttgctc agttgcagct ttttttgcat tgaaagattg attatcgaac agcatgcccc
    19741 atagactgtt ttttaaagag gcactatcat tataagaaat aggaaatgaa gatatttggt
    19801 cagcaatgtt aataatattg aagcttttct ttgtgtcaca aaactttctg ttgtattcat
    19861 tgacaaatgc ctgattgcct atcattggtg cggcaaatgc ataagtcctg aaattatttt
    19921 ttgctgaaat ttccttgcct tttatattat tgagccatgc agtggcaagg ttggccagcg
    19981 aaccaccctg actatgtcct gttataatta tgtcgtaaat accatcatcg ttaagtattt
    20041 taatcctgtc aatcaattca gcatgcataa atgccaatcc taaagagtag ccactatgaa
    20101 cggcagcgct ggtatcatca gcaaaatgat aaggaaaatc ttctccttct aaatttataa
    20161 ctccctgtgc ggggatcatt gctgcataaa tattttcgag ccagcttacc tgtttgtctg
    20221 tagaacctct gaaatgaatt acagcagtgt gatttttctt ataaatctga aatttattat
    20281 ccatgccaaa aactcctgaa gtgtaaactt tatgatagtc ttttggaatg atgtttttat
    20341 cacttttata caaatcaata aaggtgaaac tattgcagat ggcaatcata tcacgcattt
    20401 cggatttgtc aagacctgtt tgtgcattta gttgtgcaaa aatcagaagt attagcaaag
    20461 tgagccggaa tttcattgga tacaaattaa attttcgatt tgcattgcaa tgataacatt
    20521 aaggatatag ataaattgtg ttaatgatga aaagtttttc agaattatct gtagaaaaag
    20581 cgccttttgg ataagtgtgt tcattttcat ttcacaaaat gactgatagt tttacaaaca
    20641 ctattttgtc tttagcgtaa aagctatact tttacaaacc tttaaattta tatgagtaaa
    20701 atcaaattca ttccggtttt gttaaccgca ttcattctta gtgcatttca aagtaatgca
    20761 tcttccgatg gctatcaggt aaaagtaaag atcacacctg tttttaaaga ttcactttat
    20821 taccttgcta attactttgg tgacaaacaa tatattcagg attctgccaa ggcagatgca
    20881 gcagggatag tcatatttaa aggcaaagaa aaattgccgg gtggaattta tttatttgtg
    20941 gttccaggta aaaaatattt tgaactgata atagataaag agcaaaattt taccatggaa
    21001 actgacactg cagatatggt aaaaaacatg gtcgtaaaag gctctgatga caatcagaaa
    21061 ttttacaact atctgagatt cgttatggat aagcaaaagg aggcagaaac acttcaggct
    21121 tccatgcata atgctaaaac tccaaaggac agtactgatg cccttgataa attaaaggat
    21181 cttggtaaag tagtaacaga tttcaaattg aaatatatta ctgattaccc ggactttttg
    21241 ttaagtaagg tatttaaaac ctctcaagag cccgaagtgc ctgaagcacc tttgttacct
    21301 aatgggatga aagattcaac ttttgcttac cgttattaca aagcccatta tttagacaat
    21361 gttgatttta cagatgcacg tatattgaga acgcctttgt ttcacactaa gctggatact
    21421 tatatcaaga aattagtaat gcaggtaccc gacagcataa acaaagaggc agatatgttg
    21481 gtaacaaaag cccgacccaa ccccgaagta tttaaatatg ttgtttggta tcttaccaat
    21541 acctatgaaa catcaaatat tatgggtatg gatgcagttt ttgtgcacat ggccaaaacc
    21601 tactatacca aagatcaggc aacctgggtt gactcgacta cactgtataa aatacaagag
    21661 cgggtaaaag ttttagaacc tattttgatt ggtcagaaag taaagaattt gattatggag
    21721 gatacaactg gtacctataa agcattgtat aatattaaaa cgccatatac aattttattg
    21781 ttttgggacc cggattgcgg acattgccag aaagtagttc ctgaggttaa aaaactttac
    21841 gacatagtaa aaggaaaagg agcagaggtt tatgcgatat gtacagagac agaaatggat
    21901 aaatggcgaa agtttattcg cgaaaaacat ttagattgga taaatgtagc tgacccaaaa
    21961 tatcagaata atttcaggta tgaatttgat attacaagta caccacagat ttatttgctc
    22021 gatagcaata aagaaattgt tgcaaaaaaa attgatgtgg aaatcctggc agatatttta
    22081 tcaaagaaac ttaatatgaa aatagagggt ataaaaaacc cggaaaagcc ggctcattaa
    22141 aaaatgtttc tccaatattt atatgcacga ggagataggt atcagtttac gggaatggat
    22201 attttttatt ccctcttttt gctcgttttt atattttatt ttgctaaatc tataagagat
    22261 aaacacattg aagaaaaccc ttcctatcgc tattttttag gcggattgat ggcaaaaatt
    22321 gtcggaggta tcatggtggt attggtatat acgctttact attcaggtgg cgataccatt
    22381 tattatttct ttgattcaat gaccatgaca aacctgttgt ttaaaaattt catccatttt
    22441 ggtgatgtgg tgataaatgg ttgggagaat tacaaattca gttatttgga tagtaccacc
    22501 ggaacatttg tttattatag gagccctgaa tcgtttttta tagcaagaat actttggcca
    22561 tttacgcttg ttggattaca acagatggtg ccaacggtaa tgctactggc gttggtttca
    22621 tttggaggag tttggcgaat gtatcagatt tttattatgg aatttcctaa attggaacgt
    22681 ccgcttgcct atacattttt ctttttaccc tcagtttttt tctggggatc aggtatttta
    22741 aaagattcaa ttactttgag tgcattaggt tatttttttt attctttcta ctacgttttt
    22801 attgtaccta agaaggtttt taaaaatctt gtaatagtaa ttatttctgc ctggataatc
    22861 atttcgatta aagcctatat ttttattgga gtacttcccg gtgcactcat ttggattgta
    22921 agtaaattaa cgtccaagat cagaggagga tttatccgaa aagtaacttt tcctttaatt
    22981 atgataattg ttgggctttc tggattttta cttcttaata ctttgagtga ggagctaggg
    23041 aaatttaaaa ttgacagact tctggacgaa gcagttgttt cgaatacaga tttacagtcg
    23101 gattattatg gaggcaattc ttttagcatt ggtgaaattg agccaacagt tcaaagtatg
    23161 ttaattcaca tgccgttggc aattaatgct gcattattca gaccctatat tacagaagca
    23221 aggaatgtta tgatgatggt atcgggttta gaaaatctct ttattttatt atttacattg
    23281 tatgttcttt ataaggtgaa aattattggc atattcaaat atttcaataa gaatagtttg
    23341 cttacttttt cattaatatt tagtttgttt tttgcatttg cagttggtat ctcaacaagt
    23401 aattttggaa gtcttgttcg ctataggatt ccattgcttc cattttatgt atcttcatta
    23461 atcataatca gatatttgta tgaaaaagag aagacggaaa aacgtgagtt aagttatagc
    23521 tactataaag ccattacaga gtaagtatct gtaatttaca ttaataaaaa tttctattag
    23581 tatctttttc tatctgaggt ttcaggacta agtaaggatt tattttctga aaagaaggaa
    23641 gcctgtctaa aagaaaatga ctaaccaaaa ccgaattcta aatcagacag gcgctccttg
    23701 tgtgtgtttt tcatagctgc tatcatggca aaaattccat catggcttcg gaactatgca
    23761 gtaagagcat taaatagcct gaaagttgca gaggtcttga aatttatcct ttctaatcct
    23821 taattcattg aattgcagca gataatttat tggttaatag atcgcatgaa taaaggagat
    23881 gctactttac tcataaatgt ggaaaattaa ttctgttggg ctgattaaaa ttatttgttt
    23941 ttatgaaaat tacattaaat ttaccccgac taagtaagcc accactaaag caccaaagac
    24001 tacagatatc agcccatagc gaataacttt ttgaattgct tcgaatggtt ttaaaaaata
    24061 agctgagtag tattttcaat attgttcaat ccggtttatt gccggagcag aagttaagtg
    24121 ccctctttat aaaccaccaa tctaagtttc aattaatcag tctggcccat gattagacat
    24181 ttactcacca ccggaatttt tttcgtaatt tctttttctt caattgctca gaattattta
    24241 atgagcaata ccaatgtttc aacctgtgca gggaactttt atgatagtgg aggtggaggc
    24301 gcaaattatg gcctcaacca aaacactaca atgacgttgt gtagcaacac tgccggacaa
    24361 tgcatcaggg tttcttttac tgcttttaat ctggaaaata gttttgattt tttaagaatt
    24421 tataacggcc caaatacagc atcgccatta atcggatcat ataccggaac gacaagtcct
    24481 ggtgtggtta caggtacttc cggctgtttg acttttgttt ttacatcaga tggttcaata
    24541 aatcatgcag gatggacagc atcaattagc tgtgttactt gtgctgccgg tggtagttgt
    24601 cttcaaacat gtaatggtgg tccggcacct gcaaatgatg cctgcagtgg agcacaaaac
    24661 cttggtatgc ttcctgcacc tgcagcgtgt cctaatggaa taggctcgtg ggtaaatgtc
    24721 agtactacca acttatgtgc aacagctgaa atgccttata caactttgac aggatgtaat
    24781 ccttccggca atatggcaag cccagctgcg gatgtttggt atcgtttaaa tctgaccggc
    24841 cccactctca atgttcaaat atccggagga atccaaacgc ctaatctggc attatatgcc
    24901 ggtactacct gtaataattt ggttgggcga ggatgtgcaa taggggcagg gggtatttta
    24961 aatacttcat ttggtggatt ggctgcaggt acatattatc ttcaggtaag tggaggtaat
    25021 ttaaatgacc aatgtgattt taatttggca ttgcaaaata attttgattg ccagggttgt
    25081 gttattgtaa ataatctggt cgtgaatcct ccaccggtta atggaaccta tcaggcgggg
    25141 cagttggtta atttttgtta taccatttct gattataatc aaacatcagt taactggctt
    25201 catgctgtag ttccaacgtt tggtgccggt tgggatatga gtaccttaac cacggttatg
    25261 cctgctaact gttcgggtgc cggtgcttgg ggttggtata acactaacat tacaagttct
    25321 gcaaccggtg taattcatgg gccggggttc tattatgaat ctggattagg aagtccttta
    25381 ggttttatgg atggtaatcc cggtaataat ttcggtgata ataacgctac taatagttgt
    25441 aactggacat tttgctttag tgtcagaaca atgccggcct cacagtgtac tcaaggtgca
    25501 agtttaaatg tcagcattga cacatacggt gatggcgaat ccggatcatg gacatcatta
    25561 gcttgtaccg gagatcctgt gactgatttt tttgcaacac tagcttgttg tcaacctccg
    25621 attgtaaacc caaacaatcc gctttgtgat ggctatgttg gaagtgcaaa cggcatagct
    25681 caaggagtat ctccatggac ttatatctgg caaaacagtt ccggaggaac aatgatgcag
    25741 gtaaataata taaatagtgc tcatgctatt cccaatcttg cagcaggaaa ttattcactc
    25801 accactattg ataataacgg atgttcttca actacctatt tttctattac aacaccacct
    25861 gcaatagtta tagcagccaa tgcaatcgga acaaagtgta atagtaatga tggagcaata
    25921 aatatttttg caaatggagg tgtcgctcct ttacagtaca gtatagataa tggtacaaca
    25981 tttagtgctg catctgtatt taataatctt gccgcaggca gttatacggt tgttgtaaaa
    26041 gatgctaatg gctgcacatc atcgcaggtt gttagtgtag ctccatcatc aactccagtt
    26101 ataaatagta gtccggtaac gcaagtaagt tgctttggcg gtaataatgg aaatatagtt
    26161 gttaatgcag caggggggaa tcctccttta caatatagta ttgataacgg tacaacttat
    26221 caggcttcaa atagttttac aacattgact gcaggaactt attcggttgt tgttagcgat
    26281 gttgatggct gtacctccag tgtaacactc aatgtgacac aacctgcaca gataaatata
    26341 tccaatgttc taacaacaga tgcaacttgt ggcaacagca atggctcatt agttgttact
    26401 gcaagtggag gaacaggagc attacaatat agcataactg gtggggtacc ttatcaggca
    26461 tcaaatacct ttaataacct tgctgcagca aactataatg ttgtagttat tgatgcaaat
    26521 ggttgtacac aaacaatcaa tgcacaagta aataattcat ctgcacctgt tataaatgca
    26581 actaatgcaa catcactatt atgtaacggt gataataatg cttcaattac aatcaatgca
    26641 agtggtggaa ccggtgcact tcaattcagt attaataatg gtacaacatt tcaatcaggt
    26701 aatgttttta atggattagt tgcaggtact tacaatgttg ttgtcagtga tgcagtaggt
    26761 tgtcttgttt caactgctat tgttatagct gatcctccgg tattgagcat atcggcaaac
    26821 aatgttggta caacatgtac acaaagtaat ggttcaataa ctgcaagtgg tggcggtggg
    26881 tcaggcgttt tacagtatgc aataaatggt ggctcctatc agccttcgac aaactttaac
    26941 aaccttgcag caggtaacta tactgtcact gttcaggatg ccaatggttg tactgcaagt
    27001 acaacaacaa atattacaga tgccccttca gcagttattg ttaatattac aacaacaccg
    27061 gttggttgta atggtcagtc tgatggcaca gtagatattg ttgcaaattc aggaacagca
    27121 cctttgaatt attcaattga taatgggtta acgtttcagg caggaagttt tttctccgga
    27181 cttgctgccg gcagctatag tattgtagtt agtgatgcaa atggatgtac aacaacaagc
    27241 agtattaatg ttgttgaacc aacaacatta atactgaatc taaattcagt caacacaaca
    27301 tgcagccaaa ctaatggaag tgtaagtgca attgccaatg gtggtacacc tgcttatcag
    27361 tttagtattg atgcaggtgt tacttttcag gcaagtgcaa gttttggtgg attggcaaca
    27421 ggtgtttatt caatagttgt tcaggatgcg aatggctgta cagcgtctgg tagtattaat
    27481 atatcagata caccgggtcc acacattgac aatgtggctt ctgttgacat taattgcaat
    27541 ggtgcaaata gcggatcagt tactattaca gcttcaaatg gtactacacc tttgcaattt
    27601 tctattgata atggaacaac ttatcagtct ggaaatgtat ttaatagtct gtcgccagga
    27661 agttacaata tagttgtgca agatgcgaat ggctgtacaa ctacagccgc agcaaatatt
    27721 acagagccaa cagcagttgt gataagtgga acacctgtta cagatgctaa ttgcaacgga
    27781 gctaacaatg gaaatatcac catcaatgcc aatggcggaa caggagcatt gcaatattca
    27841 attgataacg gaacaactta tcagtccgga aatgtattta atagtctgtc acctggaagt
    27901 tacaatatag ttgtgcaaga tgctaatggc tgcacaacaa caggcacagc aaatattaca
    27961 gaaccaacag cagttgtgat aagtggaaca cctgttacag atgctaattg caacggagct
    28021 aacaatggaa gcatcaccat caatgccaat ggcggaacag gaacattgca atactcaatt
    28081 gataacggtg ttacatatca atccggaaat gtatttagta atttatcacc cggaagttac
    28141 aatatagttg tgcaagatgc gaatggttgc acaacaacaa ccacagcaaa tattacagaa
    28201 ccaacagcag ttgtgataag tggaacacct gttacagatg ctaattgcaa tggtgcaaac
    28261 aatggaagta ttaccatcaa tgctaatggc ggaacaggag cattgcaata ttctattgat
    28321 aacggagcaa catatcaaac cacaaataca ttcagcagtt tatcacccgg aagttacaat
    28381 atagttgtgc aagatgctaa tggctgcaca acaacaacca cagcaaatat tacagaacca
    28441 acagcagttg tgataagtg
//
LOCUS       DAOQNP010000031        28259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_536_length_28259_cov_9.906219, whole genome shotgun sequence.
ACCESSION   DAOQNP010000031 DAOQNP010000000
VERSION     DAOQNP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_28259_cov_9.906219"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PKY09_06005"
     CDS             complement(<1..782)
                     /locus_tag="PKY09_06005"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12446.1"
                     /translation="MLKKILITVWIISMTTSLQAQQKKSYQVGIIAFYNLENLFDTIN
                     QPEVNDEEFTPTGSNNYTGKVYLDKLGKLSDVLSLIGKDESPDGFSVLGCAEVENESV
                     LKDLVNEPKLKSRNLKIVHYDSPDKRGVDVALLYNPKYFTPKFSEPLFIDLRTPENPE
                     YYTRDILYVFGMYLGEPIHILVGHWPSRRGGEEASAPSRAKAAAVCKNKIDSITKINA
                     DAKIVLMGDLNDDPLSPSVTKVIKAVGSKDKVERGGMYNPWV"
     gene            complement(853..3147)
                     /gene="rnr"
                     /locus_tag="PKY09_06010"
     CDS             complement(853..3147)
                     /gene="rnr"
                     /locus_tag="PKY09_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNB12447.1"
                     /translation="MSKKNIKFTKTQLEKLKQELLQVFLQHPRATFNPKQVWRKAEIN
                     LPEEYISVVLLADKHYLDETIQSLILELYEEGELLEVQQFRYKLLPAERFIEGNIQVT
                     SKGEGYVMNDGFEEDIQIPSHLMSNALNGDRVKISLLAHREGKRQQGEVVEVISRSRT
                     QFAGTIQHSGSYAFVVCDDSKMHVDIFIPGKQLNGARNGDKVIAEITLWSADASNPEG
                     KVVRILGAPGEHTAEMNAIIVEYGLPESFPDEVEKETLRISDAISNEEIKKRRDFRKV
                     TTFTIDPVDAKDFDDALSIQQLPNGNWEMGVHIADVSHFLKPGSAMDEEAFNRATSIY
                     LVDRVIPMLPEKLSNMVCSLRPNEEKLCYSAVFEMNEKAEVMNEWFGRTVIFSDHRFT
                     YEEAQQVIETNKGPFVKEISVFHRLAQLLRKERHRKGAISFEKTEVKFRLDEKGNPIG
                     IFLKESKDSNKLIEEFMLLANRRVAEFIGKGVAKGKKEKTQKTFVYRIHDVPVEEKLS
                     DFKTFAAQFGYRISTASGQETAHSLNKMLKDVEGKKEQSMIEQLAIRSMAKAVYSTEN
                     IGHYGLAFDFYTHFTSPIRRYPDVMVHRLLDYYMSGGKSVSQQEYEEKCRHCTEREIN
                     AADAERSSIKYKQVQYLAARKDEIFKGVISGVTEWGLYVELEENKCEGMIRLRDLNDD
                     YYEYDQKNFCIVGHRSRKKYSLGDELMVVIKSTDIIKKQVDFMLYDKDRLHEVSPFKR
                     SDKKRFPSSGKIKGDKRKSGKRKR"
     gene            complement(3245..3544)
                     /locus_tag="PKY09_06015"
     CDS             complement(3245..3544)
                     /locus_tag="PKY09_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB12448.1"
                     /translation="MNTVHEITASNTNVLIDFYADWCEPCKWLNPVLEELKQQLPDLY
                     IHKINIDQLTEEAAAFEIKSVPVLILFVDGNQVWRMNGFKYSKELAKELKEFLKA"
     gene            complement(3541..4932)
                     /locus_tag="PKY09_06020"
     CDS             complement(3541..4932)
                     /locus_tag="PKY09_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB12449.1"
                     /translation="MFIQQLYTSCLAEAAYYIESDGQAAIIDPIRETTPYLSLCEQRH
                     SKITHIFETHFHADFISGHIDLAKQTGAKIVYGPEANTNYPVYNAKDGEEFKIGKLTI
                     KAIHTPGHTLESTCYLLLNENNQPHCIFSGDTLFVGDVGRPDLLDGSGISKEFLAGKL
                     FDSLNTKIKVLPDNVIVYPAHGPGSACGKNIGKETWSTIGQQKQSNYYLKENNREAFI
                     KNLTTGIMPPPMYFFEDARINKQGYQPIDEVVLRNAKPLSIEKFKEHIQQGALLLDSR
                     NADDFEKGFIPDAVNIGLNGQFANWLGTLIPIKQKVVLLTEKGKEEETVIRAARVGYE
                     NIAGYLEGGIESWKNNGQEFHTISSVDAATFTNLLKTGQYIALDVRKPGEYESSHVKD
                     SVFVSLSDIPQHYNKLDKSKTYLIYCGGGYRSMIAASLLKKNGFENIVNVYGGFGKIK
                     DCSISLETSQPVM"
     gene            complement(4996..5883)
                     /locus_tag="PKY09_06025"
     CDS             complement(4996..5883)
                     /locus_tag="PKY09_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNB12450.1"
                     /translation="MNRFSIKDIEILTGIKPHTIRIWEQRYGIIKPQRTATNIRYYTE
                     NDLKLMLNVSLLNKMGFKISKITCLKPNEIEHKVLSLVEQANDADINLSALLNCMVRL
                     DEDTFEHLLNKYLETNGLETTMTRILFPFLYRIGLLWQTGSITPAYEHFFTNLVRQKI
                     IAAIDKQVLTPLPDAKRFLLFLPPNEPHELGLLYAHYVIRQRGHRSVYMGLNLPIEHI
                     ICAHECCQPDFIVTTLTSCIKINAIEEFIVQISKTFSKSKILLSGAQAVNKSLHLPQN
                     VQIISDFDDFIQYIENRVN"
     gene            complement(5956..6909)
                     /gene="metF"
                     /locus_tag="PKY09_06030"
     CDS             complement(5956..6909)
                     /gene="metF"
                     /locus_tag="PKY09_06030"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNB12451.1"
                     /translation="MKVTDHFKKSDKTLFSIEILPPLKGKSIQSIFNAIDPLMEFKPP
                     FIDVTYHREEFVYKKREGGYLEKISIRKRPGTVGICAAIMNHYKVDAVPHIICGGFSR
                     EETENALIDLNFLGIDNVLLLRGDSEKHESHFTPEPGGHAYALDLVEQVVNMNSGTFL
                     DDDLKGVEPTKFCIGVAGYPEKHFEAPNLNTDISFLKKKVDAGAQYIVTQMFYDNQKY
                     FDFVNKCRQVGINVPIIPGLKPISTKKQTAILPKTFFIDIPDDLIQEIEKCKSDDAVK
                     QAGIDWAVTQAKELIKFGVPCLHFYTMGSPENIRKIASQLF"
     gene            complement(6989..10276)
                     /locus_tag="PKY09_06035"
     CDS             complement(6989..10276)
                     /locus_tag="PKY09_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12452.1"
                     /translation="MSKLLARLTALMLVATMFVPVISKASHLAAGDIYYTYTGTPNTF
                     LITLRLYRDCAGITMSSSETVCYTSASCNISQSITVNLVPGSGQQIPPSPCVPSAGPT
                     TCQGGTAYGIEEYLYQAVLVMPAQCIDWKFQYETCCRNGNITTLNNAAGMGFYLETTM
                     NNLDYPTNSSPHFNTIPVTQFCVNNQFYFDQGATDPDNDSITYSLINAQDASGGCPWT
                     PFDLQYNAPYSGVYPISSANGVTMDLLTGVIAFLPNLLQNGVIAVRCFEYDRITGLLK
                     TIGKREIQINIVSTCTVVTPSFDSAQVASGVNIVIDGINNVTCDDTTFYIQVNPPVQC
                     GSIVPSDIRFLDPSGLPNPVTSAVGINCVNGLTDSIMVTVLNPIPTGTSYIFTKIGND
                     GNTFLSECGSPMNEFDTLTINLYDSVPYAFDVTDTLDCTFDSLFIVSNQLIDCFTISA
                     DGSDFTLVDANGTPITITGATCLGTNGGHFGTTFLITTVPTTNAAGPLTLIVNTGSDL
                     NTIANGCGTMVLQDDTLGTIQVRNNITLSLGPDITVCASDPAPVLNAGNHPGATFDWL
                     LNGNSTGQTSQTITPTVSGTYIVNVQSSVSCFGSDTVVVNLIPSPSVNIGNDITLCAN
                     DPFPTLDAGNPGATYQWYLNDTLIAGATSQQFTPTTAGTYYVEVSTGATCLGRDTINI
                     SQVLQLSVSVNDLSYCSGNAVTLQSTSANSGVNYAWTLNGNPIAGQTGASVPVTGSGV
                     YAVTISTGSCSAIDSAIVTEVEQPTASLSNISQCDNVTLPVLNPGYVPSAGTNSVFSW
                     TLNNNPVGGNDQALNTATTGAGNYNVTITNTSNGFTCTATASMTLTINATPAVEIGNN
                     VTQCAGATPATLATANGTGNTYQWSLNNNIIVGANDTSITATSAGTYSVLVTSAGGCT
                     NTDNVVVSINALPTPEIHDELQKLDSVLFCATQNPAPVLSLTSNVFIQSYNWTLDGNN
                     LSTNANVTVAAIGEYQVTVIDSNGCSNTDVIRVIEAPCDLEIFNVITPNNDGKNDVFE
                     VKNLMDFKTRKLTVFNRWGKKVYSADPYNNDWDGGNQAAGVYYFILEYDNGIKSGDKK
                     GSFNIIR"
     gene            complement(10314..11228)
                     /locus_tag="PKY09_06040"
     CDS             complement(10314..11228)
                     /locus_tag="PKY09_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12453.1"
                     /translation="MKEDKNIEQIFKDAFSNFEVPVDSSLWQGIENSLPANPTPLTKV
                     SFLAKQAAYLKITGAVIAVATAVTAVLYFSNKNEKGTVQNAVHINNETANAVEVATQK
                     SVKQTTETTSKSEKINTPEEPKTKKPAIVAPDKTEQSDKVVENKSTPINTVPATEVEA
                     IIQQPQTDKQMISGPKTNVAAEPTKETEVKKTEMKKPEAKTSVSTESKTLPLPGLGNM
                     VFTPNGDGIGDWFEFDDSNLSEVSVKIYSLKGYKLVSEWHTHGGRWDGNLQNGNPAEE
                     GIYLVMIQAKGIDQTNYKNTIKLQLRRN"
     gene            complement(11249..11776)
                     /locus_tag="PKY09_06045"
     CDS             complement(11249..11776)
                     /locus_tag="PKY09_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB12454.1"
                     /translation="MLNEQIIQGCKDKQGSAQRQLYELVAGRMMGVCLRYAKDDEEAK
                     DLLQEGFIKVFQQIDAFRGEGSFEGWIKKIFINTALESIRKKKLLTQPLEDEEDASDN
                     FLIQENKLEAKELLQMIRKLPDGYRVVFNLYAIEGFNHREIAQQLNITEGTSKSQYAR
                     ARQFLQKMLTEEKVK"
     gene            11960..13117
                     /locus_tag="PKY09_06050"
     CDS             11960..13117
                     /locus_tag="PKY09_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB12455.1"
                     /translation="MNIYTQKQQWKLLLLASAILIGLLSLWYTNNLVKKLSEEERKKV
                     QLWAEASKQLANISVEAGDISFSLMVLQDNNTVPAILTDESGNILSHINLDTAKVNDT
                     QYVQEMLLQMKERHEPIEIAFANNQKNFIYYEDSGLLYQLRYYPYFQLAIIALFLLVS
                     YLAFSTSRKAEQNQVWVGMAKETAHQLGTPLSSLMAWIEYFKSTAQDQNHVEELEKDV
                     ARLNTITERFSKIGSAPVLRKEDVYMVIENTISYIRSRTSSKVIFSLNKAQPHQVLAP
                     LNIPLFEWVLENLFKNAVDAMDGEGKIDVMITDQLQFVYIDIADSGKGIPKSKFKTVF
                     KPGYTSKTRGWGLGLSLSKRIVEEYHNGHIFVKNSEPNNGTTFRIVLKKQV"
     gene            complement(13125..13643)
                     /locus_tag="PKY09_06055"
     CDS             complement(13125..13643)
                     /locus_tag="PKY09_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB12456.1"
                     /translation="MIIKPPSPQQLSEYYQGYLKYLPEEDMLLTLKTATDEMESMLEQ
                     IPVNKETFSYAPGKWMLKEVIGHLCDTERILTYRALCFARNDKTELPGFDENEYVANG
                     NFKTRAMHSIGIEYLIVREASLALFNSLDAADFDKTGTANQTKYSVRHMLYFCIVHQR
                     HHFNIIRERYYQ"
     gene            13720..14208
                     /locus_tag="PKY09_06060"
     CDS             13720..14208
                     /locus_tag="PKY09_06060"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNB12457.1"
                     /translation="MSGNLLLRYANYNKWANQRITNAIVSAGETVADTEMKSSFNTVR
                     KTIYHIYDSQHTWIMRIQNKPYSWPPSKDFKGDLNEFSKLLIESSQEWINYISNLNDE
                     DFKKIISYKNTKGIDHQTPLEEIIMHCFNHSTYHRGQLVTMLRQAGVNSFEPMDFIAF
                     VR"
     gene            complement(14189..15184)
                     /locus_tag="PKY09_06065"
     CDS             complement(14189..15184)
                     /locus_tag="PKY09_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB12458.1"
                     /translation="MYLITGASGLVGSQLVFDLISMGRSVRILLRKSSSTVLIKKKFK
                     SSPELFDKIQIAYGDVLDIFSIEDALQDCTHVFHCAAMVSFNPQKRKEMYKVNADGTA
                     NVVNACLEKRNIKLGFVSSVATLGRGTQQMINENDWFNQNEKNSYYAISKYAAEREVW
                     RGITEGLNAVIINPSVILGDGNWRSDSSSLFNTLYSGLKFYTQGVSGFVDVKDVSRSL
                     ISLTESEISGERFIISSENKSYEWLFKTMAKGLNIKPPTIKAGVFLSQLAWRLEWLKS
                     VFTGKPSLITNETAYTSQQIYNYDNSKITEQLNYRFIPVEETVDRVCKIFIAQKQ"
     gene            complement(15187..15606)
                     /locus_tag="PKY09_06070"
     CDS             complement(15187..15606)
                     /locus_tag="PKY09_06070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNB12459.1"
                     /translation="MTEQQRIDSGKAPEPVGLYPHARRVGNLLFLSGVGPREKGSKKI
                     PGVEQDASGNIVQYDIAEQCHAVFKNVRYILEDSGSSWDKIIDVTVFLTNMKDDFQIY
                     NKLWAEYFKENPPCRTTVEINCLPTPIAIELKVIATI"
     gene            complement(15603..17222)
                     /locus_tag="PKY09_06075"
     CDS             complement(15603..17222)
                     /locus_tag="PKY09_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNB12460.1"
                     /translation="MPLNKIISFKSIFLWTAVLLMLGACASVVAPTGGKKDETPPKLK
                     VVVPPEQTVNFNGERVVFTFDEYIQLLDKDKQLVISPIIEPAPTFEVKGKKLTITFKK
                     KLKENTTYTINFGNAVADLHESNPLAGFRYVFSTGTYVDTLFVKGNVVFAENLKSEKG
                     ILVMLYTLDDDSLPYNNPPSYFTRTDESGNFSIKNIGQGPYKIFALKDADNNYKFNQS
                     DEWIAFDKTPVSGGDSTPVNLRMFKEADKKVYVKESKTINAFAFSVLMNRPVGVLNIE
                     PLQTADNIKLTNRSKTNDTLVVWLKDSISDTLSYKLSSENNWTDTLTISKPPKALRGH
                     LKQTISLLSSTNIYPGNDVLLKTTIPVASVNKEKVLLKRDSTTAQPFEIAFFDSASSF
                     ITIKAGTIEKSNWNLTLQPGAVTDIYGNKNDTTKLNFHTNEFTDYGSVKIKVDGLSGT
                     NYILQVVDEKDMVISEKYIGGNGSYLFERMLPSKCKIRIINDADGNKTFTTGNYLLKQ
                     LPELVYYYKETLTLRANWDIEINWNIKEIKQ"
     gene            complement(17209..17715)
                     /locus_tag="PKY09_06080"
     CDS             complement(17209..17715)
                     /locus_tag="PKY09_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNB12461.1"
                     /translation="MSEAIVIDTTLSKQEKYAQLLPQAEALIKGEVDAVANAANICAM
                     LKQVFGFFWVGFYFVKKHEGKQQLVLGPFQGPVACTRIELGKGVCGAAWQQKKTIVVP
                     DVNNFDGHIACSSLSRSEIVVPVLKHDVVAVIDIDSEHLNHFDETDKYYLEKISSLYS
                     ETFSYAAE"
     gene            complement(17746..18429)
                     /locus_tag="PKY09_06085"
     CDS             complement(17746..18429)
                     /locus_tag="PKY09_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNB12462.1"
                     /translation="MIKDFFKLFFPDNCPGCGQSFLRGEKHVCLQCLYGLHKTQYHND
                     PENPIAKNFWGKTDFVAVTAVYFFQKGQVLQRLLHQLKYNGCKEIGIMLGRQMAIELF
                     QASAFSNADIIIPVPLHPGKLLQRGYNQSEMIALGIAENWQKEVHTATLKRKKYTQSQ
                     THKARYERFENTESVFELDDVKPYENKHVLLVDDVISTGSTIISCAEAFSGINGCKIS
                     MASVAAPHR"
     gene            18635..19570
                     /locus_tag="PKY09_06090"
     CDS             18635..19570
                     /locus_tag="PKY09_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12463.1"
                     /translation="MEESVNEHTKADNREQNSRRALVFIVITILLGVNGLLLYQFFDK
                     KSHLEEVTRTLDNTSAERDALSAELQQVKAEFEKINQENSSLQAQLLSKDEEIKSKMA
                     QIRRMIESGDATQLKVAREELERLRSMNQVYVAQLDSMRNANVALASQNESLNDMITV
                     EKNKSTALALENSTLTKRVAAASVLRTTSIKASGVKYKSSGKELETNKASSTQKIKTC
                     FTILENTVTTSGSKDIYIKIMSPDGVVMTNNTETFTIAGQTSLYSLKESFDYENREMN
                     MCVYWEKGTAFSPGKYTVEAYCEGNLIASTPVVLK"
     gene            complement(19578..23045)
                     /locus_tag="PKY09_06095"
     CDS             complement(19578..23045)
                     /locus_tag="PKY09_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12464.1"
                     /translation="MIRLSLVFFLFLSVTLGYGQSLSNLRIATIGVNSDTLQLDSLSI
                     VPGSFHLIDSVAWDISCIKVLYEKGILIINKSCKQKNSDSLRISYRVFPVNFSKPHSN
                     RNLSVIKQKYSGQYNPFAYDNAENNSQSIFNFDGLNKSGSISRGISFGNNQDVIVNSG
                     LNLQLSGTLSDRVEILAAITDNNIPIQPEGNTQQLQEFDKVFVQLSRDSTRLIAGDFE
                     ISKPYGYFMNMYKKAQGAMVSSSFFIDQKSMWVMKTTDAAAISKGKFARNVFNGMEAN
                     QGPYRLTGAENETYIIVLSGTEKIYLNGILLTRGKENDYVIDYNTAQITFSARRLINK
                     DSRIVAEFQYSDKNYVRTLLYSSNIFESKWAKFHVNYFTEQDNKNQPLFQDLNEDKRQ
                     VLANAGDNLQNAYFLNADSVGFNRNEVLYQKDSITIDSIQYIYFTYSIDSTRAFYRLG
                     FSNVGQGRGNYRLLNSNVNGKVYEWIAPVNGQPQGHYEPVRLLIAPRRQQLLTAGGDV
                     ALSKNTNVSVEGAFSKNDVNLFSRKDKSNDVGYALHAAVNHSMPVSKQDSSWKWVSVV
                     SAELTDRRFRPLENYRDVEFVRDWNLTSAFDTLNETFLSINTGLEKNVAQNIFYQLKY
                     FTKGNQYKGLMNMISSNYNWNKYFIQYNGSLLNTTGAQSNTVFLRSYVDGGKSINKLK
                     VGYRQENERNKMNVAGIDSLMSNSFGHDKAGIYFTSADTGKWSYKAEASRRWDYRPSD
                     NNLKLATTADQADADVAFIAGRGKRITLGSTYRTILVNRKEITTQKKDESFLGKLEIA
                     LQFLNGGITSNTYYEVGAGQEQRQAYSFIKVAAGAGVFEWNDYNGNGIAELNEFEVAA
                     FQDRAEYIKIFTPTNDYVRIQTNQLSEVIGVNPSAFIKNKQGKTKLINRFALQSALRF
                     DNKLLSGKWADGLNPFGYNINDTSLLITNSTLRNTLFFNRSSSVFAADFNYDNISNKS
                     LLANGTEGRNLTSWLLNTRTYFKRVYGVTTALKYETKQNSSVFTNRNYEIAGYAIEPR
                     FSVQPGTVFRTTLIYKYQDKQNVIELANEHATITTIGAECKYSSFKNGVASLGVNIIS
                     IGYNADVNSPLAFEMLEGLKKGRNYTWEISLQKNLTGNLQINIGYNGRKPQGDKIIHT
                     ANVQARAIF"
     gene            complement(23286..27596)
                     /locus_tag="PKY09_06100"
     CDS             complement(23286..27596)
                     /locus_tag="PKY09_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12465.1"
                     /translation="MKRKLFISTLLLLSSLNSFSQCEYIEDSTALRTIGNISATLCNS
                     NFTYCSKNGFSLFPHTNYRVLNIMINIIYDVNPSWDPYYQATPGANDWNPGTPNTINS
                     NPPAYLSSFMDQDFNTLPPQGIMTRKYYESSFGALHIVGDFIVVDVPHSYIINNSGIQ
                     PPLYLFSSYTLLQNAINIINASGGLSAIFGHNSINDYDYCALGFPGISKNLTSNNMID
                     FVQILFRNSTSNYGGLKLGQGYTGGGLSSHILIGSNFYTGEVISVQAVGKSPLTNPNG
                     IIIHELAHNLFGGNNFHTSGGHHYGSQPSTTFLGIQGGYGLMGSAGAGLTSVNGYERY
                     RMGWYSSTYNPNQIPIQANGTASDIDQSMGAQTFVLRDFMTTGDVVRIKLPYKDVNAL
                     DQYIWLENHKIGSNGMLDYLQFSDSDPCRPQGTPGIYAYMQIGKNILESNNQLDVYPS
                     GEADNLKVISAEGNFDHEQIANQSISCVAWPTSESYHLLESNTLTGDNDQMHIFYNTL
                     TNTINNSNGHAPFIQQRLNNSFDSNIPYLGDNKDAFTNGTVMEIGSNPSPVNLPTFYY
                     ALSGSTFYPQTNAPNNRHTYLTGLKISFTQLTGGDFQVDVVWNEYDVKNDVRWCGDII
                     LKEQVNLKANKIITLDQSRTANQINRDAVSGEFTPITTFTCEGGSYFHIESNSQLILE
                     NNSVLHLETGSQLSMESGSIVRVKNGCKLILDSKSTVINNGGRIIIEDGGTLTYQEAD
                     ITLATTNSTLEIAGLLDLKSPVTAAYNIFTFSGLGYLLFNSPLSPSANVACTPGDCIF
                     QLVGGTGQLLLKVTQESLRLPVGLAKFIVEGGVCQFTGYSAARVLGATTKTTLWGTVF
                     TSTTGNYNTHKGLMLYGGAAAVSYSTFLHGYQGIRNLNGDFTVEYSTFQNCGVGVLTD
                     GGSFDINNSVFNQNRGFGISADNMTLPCLLTSNEIKYTTLTNGIGVFFDGSLTSSLYA
                     ENNYIHDNIVGMQIKNTQLKGICNSVKNNQTNGIEINQGATLDFSGSPTLKPSNAIQN
                     NDISLKLSVAKNLFLADGYNSLTPIQSGVQKAVRGTIKKTCPNPSAINAIYNEWNTNG
                     TFSTNDYALTTVAGQSGCATAIPVTINDPNPIPFVSCSNTQPLVAAPENPLYDCNNCE
                     TIYTSNFNGDKLNEAGVAALQQTTETGGSSDAQAVTLFNEMLMYNLPTPDDKEKYILN
                     YSYSKMGTALGAAFKNNELSITDNTPALSSEVQQMMEVLNKHLAEAIGNGDTLDRFYY
                     SLDKANVYRLAGRRDLAFPILDDMFNWTTASDELDYLNYKNCITHLEQDLIDSIITAE
                     EYMDASLICGQQSSGLRVLSSTIVKQDKSLMAISSTYAAQNITVSPNPATDRLNIYYT
                     QEQPLTIVIKEVTGREIHRSVLNSHSSLDCSQFAKGIYTLQLFDKENIRVYKSKVLLK
                     "
     gene            complement(27593..>28259)
                     /locus_tag="PKY09_06105"
     CDS             complement(27593..>28259)
                     /locus_tag="PKY09_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12466.1"
                     /translation="ENKMSDGFAYDWYTKYDIRYCDSTSLYGVFYNFFNDPLAYTIKF
                     YYYTTPIIAKISIFRDSIFLPDSADKGCFCSYNPIFNPNSHNRNPNGWVRTWRDSVQT
                     HIGYYPYDAYINPSLVDTLDAPDIFYYKFIPQHIIVGLEEFNNQNSDYKIYPLPFFNI
                     INIEFKDNKDFYYRIINKEGIIIKGGKLQNKQIPALDIKDGIYTLILFNKTETVSFKI
                     IKI"
BASE COUNT         8381 a         5489 c         5069 g         9320 t
ORIGIN      
        1 tcaacccatg ggttgtacat tcctccacgt tctactttgt ctttacttcc aactgcctta
       61 attacttttg taacacttgg gcttaaagga tcgtcattta aatcgcccat gagaacaatt
      121 tttgcatcgg catttatttt tgttatagaa tcaattttgt ttttacatac agcagcagcc
      181 ttagcacgtg atggtgcaga agcctcttcg cctcctctgc gtgatggcca gtgaccaacg
      241 aggatatgga taggctcgcc cagatacatg ccaaaaacat agagaatgtc acgggtgtag
      301 tattcaggat tttccggagt acgtaagtca ataaacaaag gctcactaaa cttgggagta
      361 aaatattttg gattataaag taatgccaca tcaacacccc gtttgtcagg actgtcgtaa
      421 tgcacaattt taaggttgcg tgattttagt ttgggttcat taaccaaatc ttttaaaaca
      481 ctttcattct ctacttctgc gcatcctaaa acagaaaatc cgtcaggtga ctcgtctttt
      541 ccaatcagtg ataatacatc agaaagttta cctaacttat caagatatac tttacctgta
      601 taattgttag atccggtagg agtaaactct tcatcattca cttcgggttg attaatggtg
      661 tcgaacaggt tttctaaatt gtagaaagca ataataccaa cctgatagct tttcttttgc
      721 tgagcctgaa ggcttgtagt catggaaatt atccagactg taataagtat cttttttaac
      781 atggcacaaa tctaaaaaac gtcacaatgc tgtggcttga aattatgatg attaaatttt
      841 tttttgaaaa aattatcttt ttcttttgcc gctcttcctt ttatcacctt tgatcttgcc
      901 tgatgaaggg aatctttttt tatcactacg tttaaatggg gaaacttcat gtaaacgatc
      961 cttgtcatag agcatgaaat caacttgttt ttttatgatg tcggtgcttt taatgactac
     1021 catcagctca tcgcccaatg aatatttttt gcgggagcga tgtcctacaa tgcaaaagtt
     1081 tttctgatcg tattcatagt agtcatcatt caggtcgcgc agcctgatca tgccttcaca
     1141 tttattctct tctaactcca cataaagacc ccattccgta acaccggaaa ttacaccttt
     1201 aaatatttca tctttacgtg ctgcaaggta ttgaacctgt ttgtatttaa ttgatgaacg
     1261 ttctgcatcg gcagcattaa tttcgcgctc cgtacagtga cggcattttt cttcatactc
     1321 ctgttgtgaa acactcttgc cgccactcat ataataatcc agcaagcggt gcaccatcac
     1381 atccggatac cgccttatgg gcgaagtaaa gtgggtgtaa aagtcgaatg ccaaaccata
     1441 gtgaccaata ttttcagttg aataaactgc cttagccata ctgcgtattg ccagttgctc
     1501 aatcatactc tgttcctttt tgccttccac atccttcagc attttgttga gcgaatgagc
     1561 agtttcttgc cctgatgcag tgcttattct atagccaaac tgtgctgcaa aagttttaaa
     1621 gtcgctcagt ttctcttcaa caggcacatc gtgaatacgg taaacaaagg tcttttgtgt
     1681 tttctctttc tttccctttg caacaccttt accaataaac tctgcaacac gtctgttggc
     1741 aagtaacata aactcttcaa tcagtttatt ggagtcttta ctctctttaa gaaatattcc
     1801 aatagggttt cctttttcgt caagcctgaa ctttacttct gttttttcaa aactgattgc
     1861 tcccttacgg tgacgctctt tacgaagtag ctgtgctaac cgatgaaaga cacttatttc
     1921 tttaacaaaa gggcctttgt tggtttctat aacctgctgt gcttcttcat aagtaaagcg
     1981 gtgatcagaa aaaattactg ttcttccaaa ccactcattc atgacttctg ctttttcatt
     2041 catctcaaaa actgcagagt aacaaagttt ttcctcatta ggtcttagag agcaaaccat
     2101 atttgacaat ttttcgggca acataggaat aactctgtca accaaataaa ttgaagttgc
     2161 tctgttgaag gcttcttcat ccattgcact gcccggtttt aaaaaatgtg aaacatctgc
     2221 aatgtgcaca cccatttccc agttaccatt aggcagctgc tgaatactta atgcatcatc
     2281 aaaatctttt gcatcaacag ggtcaatagt aaaagtagtt acttttctga agtctctgcg
     2341 ttttttaatt tcttcatttg aaatagcatc agaaattctt aatgtttctt tttctacttc
     2401 atcaggaaaa gattccggaa ggccatactc aacaataata gcattcattt ctgcagtatg
     2461 ttcaccggga gcacctaata tacgaacaac ctttccttcg ggattgctgg catcggcaga
     2521 ccataatgtt atctctgcaa ttactttatc gccattgcgg gcaccgttga gttgttttcc
     2581 gggaataaaa atatctacat gcattttgga atcatcgcat actacaaatg cataactgcc
     2641 cgagtgttgt atagtaccgg caaattgcgt tctgcttcga ctgataactt caacaacttc
     2701 tccttgttgt cttttacctt cgcgatgagc taataaacta attttaactc tgtcgccatt
     2761 caatgcattt gacatgagat gtgatggaat ctgtatgtct tcttcaaagc catcgttcat
     2821 tacataacct tcgcctttgg aagttacctg tatgtttcct tcaataaacc tttctgccgg
     2881 taataactta taacgaaact gttgaacctc cagcaattct ccttcttcat acaattctaa
     2941 aattaaagat tgtattgttt catctaaata atgtttatca gcaagcaaca cgactgaaat
     3001 atattcttcg ggcaaattta tttctgcttt gcgccaaacc tgttttggat taaaggtagc
     3061 acgtgggtgt tgcagaaaga cctgaagaag ttcttgtttt aacttttcaa gttgtgtttt
     3121 tgtaaatttt atgttcttct ttgacatgtt ttcgtttgat tgtaaaaata catgtagtga
     3181 aacagacgtc agacaatcta cactttaaaa aataaatgtt ttcaacagct attaatgtaa
     3241 gatttcaggc ttttaaaaac tcctttaatt ctttagctaa ttctttagaa tatttaaatc
     3301 cattcatacg ccatacctga tttccgtcaa caaaaagtat cagcacaggt acactcttta
     3361 tttcaaatgc agcagcctct tcagtaagtt ggtcaatatt gattttatgg atatataagt
     3421 cgggaagttg ttgctttaac tcttccaaaa caggattaag ccacttacat ggttcgcacc
     3481 aatcggcata aaaatctatc agcacatttg tattggatgc tgtaatttca tgaactgtat
     3541 tcacatcaca ggttgtgaag tttctaatga tatgctgcaa tctttaatct ttccaaagcc
     3601 tccatataca ttaactatat tttcaaaacc attcttttta agcaacgatg ccgctatcat
     3661 actgcgataa cctccaccac aataaatcag atacgttttt gatttatcca gcttattgta
     3721 gtgttgtgga atgtcactta acgacacaaa gactgaatct tttacatgtg atgactcata
     3781 ctcaccgggc tttcgtacat ctaaagcaat atattgtcct gtctttagca aattggtaaa
     3841 tgtagctgca tcaacagaac tgatggtatg aaattcctgt ccgttatttt tccaactttc
     3901 tattccccct tcaagataac ccgcaatgtt ttcataacct acacgtgcag cacgaatcac
     3961 tgtttcttct tcttttcctt tttctgtgag taacactacc ttttgcttta tgggaattaa
     4021 tgtcccaagc cagttggcaa actgaccatt gagcccaata tttactgcat caggaataaa
     4081 ccctttctca aaatcatcgg catttcttga atcgagtagt aatgctcctt gctgaatgtg
     4141 ttctttaaat ttttctattg acaatggttt ggcatttcgc aacaccactt cgtctatagg
     4201 ttgataacct tgtttgttga ttcgggcatc ttcaaaaaaa tacatgggtg gtggcattat
     4261 gcccgttgtc aggtttttaa taaaggcctc tctgttattt tcttttaaat aatagttgga
     4321 ttgtttttgc tgacctatgg tactccatgt ttcttttcca atattttttc cgcaagcaga
     4381 tccgggtcca tgtgccggat aaacaattac gttatcaggc agcactttaa tttttgtatt
     4441 caaagaatca aacaatttac ctgctaaaaa ttcttttgaa ataccacttc cgtctaataa
     4501 atcaggtcgg cccacatcgc ctacaaacaa agtatctcca ctgaaaatac aatgtggttg
     4561 attattttca ttgagtaata aatagcatgt tgattctaaa gtatgtccgg gtgtgtgaat
     4621 agctttgatg gttagttttc caattttgaa ctcttcacca tcttttgcat tataaacagg
     4681 atagttggtg tttgcttcag ggccataaac aatctttgct cctgtttgct ttgccagatc
     4741 tatatggcca ctgataaaat cggcatggaa gtgtgtttca aaaatatgtg tgattttaga
     4801 atgccgctgc tcgcatagtg aaagataagg tgttgtttca cgaatgggat ctataattgc
     4861 agcctgccca tctgattcaa tataataggc tgcttcggca agacaacttg tatataattg
     4921 ttgaataaac ataattctat taggatttaa aacaaagcta cactttttac agcaatgaat
     4981 aaaataaagt attgattaat taaccctgtt ttcaatgtat tgaataaaat catcaaagtc
     5041 agagattatc tgaacatttt gtggcaggtg taaactttta tttactgcct gtgcaccgga
     5101 aagtaatatc ttgctttttg aaaatgtctt tgaaatctgt acaataaatt cttcaatggc
     5161 gttaatctta atgcaagatg ttaaagtagt cacaataaaa tcgggttgac aacattcgtg
     5221 agcacaaatg atatgttcaa tcggtaaatt gagccccata taaactgatc tgtgaccacg
     5281 ttgtctgatt acatagtgtg catacaaaag tcctaattca tgaggttcgt ttggaggcag
     5341 aaagagtaaa aatctttttg catcaggcag tggtgtcaat acttgcttgt ctattgcagc
     5401 aataattttc tggcgcacaa gattggtaaa aaaatgctca taagcaggtg tgatggagcc
     5461 tgtctgccaa agcaatccta ttctgtataa aaaaggaaac agaattcttg tcattgtagt
     5521 ttccagtccg tttgtctcca gatatttatt taacaggtgt tcaaatgtgt cctcatcaag
     5581 cctgaccata caattcaaca atgcactgag gttaatatca gcatcattgg cttgttctac
     5641 taacgacaaa actttatgct cgatttcatt cggtttaaga caggttattt tcgaaatctt
     5701 aaatcccatc ttattcaaca aagaaacatt gagcatcagt ttcaaatcgt tctctgtgta
     5761 atagcgaata ttggttgccg ttcgttgtgg cttgataata ccataacgct gctcccatat
     5821 acgaatggta tgcggcttta ttcccgtaag aatttctatg tctttaatcg aaaacctatt
     5881 caacggagtg gggtttgtgg tataaacact tgtcgtgttt aaaagtttca gccttataca
     5941 tggatacctg aaacattaaa acaattgtga tgcaattttt ctgatattct ccggactgcc
     6001 cattgtataa aaatgcagac agggtacacc aaattttata agctctttag cttgtgtcac
     6061 tgcccaatca atacctgctt gctttacggc atcatcactc ttgcattttt ctatttcctg
     6121 aatcaggtca tcaggtatat caataaaaaa agttttaggt aagatagcag tttgcttttt
     6181 cgttgaaata ggtttaagcc ccggaattat tggcacatta atgcctactt ggcgacattt
     6241 gtttacaaaa tcaaagtact tctgattgtc ataaaacatt tgcgtaacta tatactgagc
     6301 tcctgcatca acttttttct tcagaaaaga gatgtctgta ttcaggtttg gcgcttcaaa
     6361 atgtttttcg ggataaccgg ccacgccaat acaaaatttt gttggttcta ctcctttaag
     6421 atcatcatct aaaaaggttc ctgaattcat atttactact tgctcaacca aatcaagagc
     6481 ataggcatgt ccaccgggct caggtgtaaa atgcgattca tgtttttccg aatcacccct
     6541 tagcaaaaga acattatcta ttcccaaaaa gttaaggtca attaatgcgt tctctgtttc
     6601 ttcgcgtgaa aatcctccgc agataatatg ggggacagca tcaactttat aatgattcat
     6661 gatggctgca caaatgccaa cggtgccagg acgttttctt attgaaattt tttcgagata
     6721 accaccttca cgttttttgt aaacaaactc ttcgcggtga tatgtaacat caataaaagg
     6781 aggtttaaat tccatcaaag ggtctattgc attaaaaata gactgaatgc ttttcccttt
     6841 taaaggtggt agaatttcaa tactaaataa ggtcttgtct gacttcttaa aatgatcggt
     6901 tactttcata aaatatttgc aacagtatta aaaaaacagg agcgttcttt tcaagaacgc
     6961 tcctgcaaaa ttataaacta tcggcaactt aacgaataat attaaacgat ccttttttgt
     7021 caccggattt gataccattg tcatactcca gaataaagta ataaactccg gcagcctgat
     7081 tgccgccatc ccaatcgttg ttatatgggt cggcactata aactttctta ccccaacggt
     7141 tgaatactgt aagtttacgt gtcttgaaat ccattaaatt cttaacctca aacacatcgt
     7201 ttttaccatc gttatttggt gtaataacgt taaatatttc aaggtcacat ggtgcttcaa
     7261 ttacacggat aacatctgtg ttgctacagc cattagagtc aattactgtt acctgatatt
     7321 caccgatagc agccactgtt acgtttgcat tggtagataa attgttgcca tccaatgtcc
     7381 agttgtagct ttgaatgaat acattgcttg ttaagcttaa aaccggagca gggttttgtg
     7441 ttgcacagaa aagtacgcta tctaacttct gcaattcgtc atgaatttcg ggagttggta
     7501 atgcattgat agaaaccact acgttatctg tattggtaca tccgcctgca ctggtaacta
     7561 aaacactata ggtaccggca gaagttgctg ttatgcttgt atcattagca ccaactatga
     7621 tgttgttgtt tagtgaccat tgataagtgt tacccgtacc atttgctgta gcaagtgttg
     7681 ccggagtagc acctgcacac tgtgtaacat tatttccaat ctcaacagct ggtgttgcat
     7741 tgatagttaa tgtcattgaa gcagtagctg tacaagtaaa tccgtttgat gtgtttgtga
     7801 tggtaacatt ataattacct gcacccgttg tggcagtatt taaagcctga tcatttccac
     7861 caacaggatt gttgtttaat gtccagctga atactgagtt agtacctgca gaaggcacat
     7921 atcccggatt cagaacaggg agtgttacat tatcgcactg tgaaatatta gataatgatg
     7981 cagtaggctg ttctacttca gttactatag ctgaatctat tgcagaacaa gatcctgttg
     8041 aaatggttac tgcgtaaacg cctgagccgg taacagggac agatgctcct gtttgacctg
     8101 caataggatt accgttcaaa gtccatgcat agtttacacc gctgttggct gatgttgact
     8161 gaagcgtaac agcattaccg ctacaataag aaagatcgtt aacagaaacg ctcaattgaa
     8221 gtacttgtga aatattaatt gtatcacgtc ctaagcaagt agcacctgtg ctcacttcaa
     8281 catagtaagt acccgcagtt gtaggtgtaa attgttgtga agttgcacct gcaattaatg
     8341 tatcgttaag ataccactgg taagtagcac cgggattgcc tgcatctaat gttgggaaag
     8401 gatcatttgc gcaaagtgta atatcattac ctatgtttac ggaaggtgaa ggaatcagat
     8461 tcaccactac tgtatcgcta ccaaaacatg aaactgagga ctgtacattt acaatatatg
     8521 ttccgcttac tgttggtgtt atggtttgtg atgtttgtcc tgtagaattt ccattgagca
     8581 accagtcaaa agttgcaccg gggtgattac ctgcatttaa aacaggtgca gggtcgctgg
     8641 cacaaactgt aatatctgga cctaaactca atgtaatatt attacgaacc tgaatcgttc
     8701 caagagtatc atcctgtaat accatggttc cgcaaccatt ggctatagtg tttaagtcac
     8761 taccggtatt gactattaag gttaatggtc ctgcggcatt ggttgttgga actgtagtaa
     8821 tcaggaaggt agtaccaaag tgaccaccgt tagtacccaa gcaagttgct ccggtaatcg
     8881 taattggagt accgttagca tcaaccaaag taaagtccga accatctgca cttatggtga
     8941 agcagtcaat caattgatta ctcacaataa ataatgaatc gaaagtacaa tccaatgtgt
     9001 ctgttacatc aaaggcgtag ggaactgaat cataaagatt aatagttaaa gtgtcgaact
     9061 cattcatagg cgagccacac tcactcagga aagtgtttcc atcattacct attttggtaa
     9121 atatataaga tgtacccgtt ggaatcgggt taagtacggt taccattatt gaatctgtaa
     9181 gaccgttaac acagttaata cctactgctg aagttacagg atttggaagt ccgctggggt
     9241 caaggaatcg gatatcagaa ggaacgattg aaccgcattg tacaggtgga tttacctgaa
     9301 tataaaaagt tgtatcgtca caagtaacgt tattaatacc atcaataaca atatttacac
     9361 cgctggcaac ctgtgcactg tcgaaactgg gggttactac tgtacaagtt gaaacgatat
     9421 tgatttgtat ttcgcgcttg ccgattgttt ttaataaccc tgttatgcgg tcgtactcaa
     9481 aacaacgaac tgcaattaca ccattctgta ataaattcgg taaaaaagca attacaccgg
     9541 tcaacaaatc cattgtaaca ccattagctg atgaaatagg atatactcct gaatagggtg
     9601 cattgtattg aagatcgaat ggtgtccaag gacaaccacc ggatgcatcc tgtgcattga
     9661 taagagaata agttattgaa tcgttatcag gatcagtagc accctgatca aaatagaact
     9721 gattgtttac acaaaactgt gttacaggaa tagtattaaa gtgtggtgag ctgtttgtcg
     9781 gataatcaag attattcatt gtggtttcca gatagaaacc cataccagca gcattattaa
     9841 gcgttgtgat attaccatta cgacaacatg tttcatactg gaatttccag tcaatacatt
     9901 gtgcaggcat taccaacaca gcctgataca gatattcttc aataccataa gctgtaccgc
     9961 cctgacaggt tgttggacct gctgaaggca cacaaggaga tggtggaatt tgttgtcctg
    10021 atccgggaac taagtttacg gtaatagact gactgatatt acaggatgct gaagtgtagc
    10081 aaactgtttc agacgaagac atggtgatac ccgcacaatc tctgtataga cgaagcgtta
    10141 tcaaaaacgt atttggggta cctgtgtagg tataataaat atcccctgca gcaaggtgtg
    10201 atgccttact gatgacaggt acaaacattg ttgctaccag catcaatgct gttaatctgg
    10261 ctaaaagttt actcatgatt atttgatgtt tagtttttat actttagttt attttaattt
    10321 cttcttaatt gcagttttat tgtgttttta taattagtct gatcaattcc tttggcctga
    10381 atcatcacca aataaattcc ttcttcggca ggatttccgt tttgaagatt gccatcccat
    10441 ctgccaccgt gtgtgtgcca ctcactcacc aatttatatc cttttagtga ataaatctta
    10501 acagacactt cactcagatt gctgtcatca aactcaaacc aatctcctat gccatcgcca
    10561 ttgggtgtaa aaaccatgtt gccaagaccg ggcaaaggta atgttttact ttctgtgctg
    10621 actgatgttt tggcttcggg cttcttcatt tctgttttct taacctcagt ttcttttgtt
    10681 ggctcagcag ctacattggt tttggggccg gatatcattt gtttatctgt ctgtggttgc
    10741 tgtataattg cctccacttc agtagccgga acagtattaa tcggagtaga tttattttca
    10801 acaactttgt ctgattgctc agtcttatca ggtgcaacaa ttgcaggctt tttagttttt
    10861 ggctcttcag gcgtatttat tttttctgac tttgaagttg tttcagtggt ttgtttcaca
    10921 cttttctgtg ttgctacttc aactgcattg gcggtttcgt tatttatatg aactgcgttt
    10981 tgtacagtac ctttttcatt cttatttgaa aaatataaca ccgctgtaac ggcagtggca
    11041 acagcaatga ctgcacctgt aattttcaga tatgcagctt gttttgctaa aaatgacacc
    11101 tttgttaaag gagtaggatt tgcaggtaat gaattttcta ttccttgcca taaagaagaa
    11161 tcaacaggca cttcaaagtt gctgaacgca tctttaaaaa tctgttctat gtttttatct
    11221 tctttcattt tcaatccttt gcggataatt attttacttt ttcttctgtc agcattttct
    11281 gcaaaaattg tcttgcccga gcatactgtg actttgaggt tccttctgta atattcaact
    11341 gctgtgcaat ttctctgtga ttaaaccctt caatagcata caggttgaaa accactctgt
    11401 aaccatcggg caactttctg atcatctgaa gcaactcttt ggcttccagt ttattttcct
    11461 gaatcagaaa attgtcactt gcatcttctt catcttccag cggttgcgta agcaatttct
    11521 tttttcttat gctctctaac gctgtgttaa taaagatttt tttaatccaa ccttcaaaag
    11581 aaccctcgcc tctaaaggcg tcaatctgct gaaacacttt aatgaaccct tcctgcaaca
    11641 agtctttagc ctcttcatca tctttggcat aacggaggca aactcccatc atgcgtcctg
    11701 ccacaagttc ataaagctga cgttgtgcgc tgccttgttt gtctttacaa ccttgtatga
    11761 tttgttcatt gagcatttct gctattatct ttaaagatgc aaaagtaggg gtaagggttg
    11821 catcactttt aattttttgt aaaaagaatt gctgttattt tgaaagaaaa gaagtattat
    11881 tgcacctgta atccaattaa ttcatccgaa tatagataga cttttacctg aactgtaagt
    11941 acgatttatt ttaaaagggg tgaatattta cacacaaaaa caacaatgga agcttttgct
    12001 tttggcctca gccatactca tagggctatt gtcattgtgg tacaccaata atttagtaaa
    12061 aaagttaagc gaagaagagc gtaaaaaagt tcagctctgg gcagaagcat ccaagcaact
    12121 tgccaatatt tctgtcgaag caggcgacat cagtttttcg ctcatggttt tgcaggataa
    12181 caatactgta ccggctattt taaccgatga gagtggaaat attttatcgc acatcaatct
    12241 tgatacggca aaagttaatg acacgcaata tgttcaggaa atgctcctgc aaatgaaaga
    12301 aagacatgag cctattgaaa tcgcttttgc caacaatcag aaaaacttta tttattatga
    12361 agattccggt ttattgtatc aacttcgcta ttacccgtat tttcaattgg caattattgc
    12421 tttgttttta ttagtaagtt atctggcatt cagcacttcg cgcaaagcag aacaaaatca
    12481 ggtttgggtg ggaatggcaa aagaaactgc acatcagctg ggaacacccc taagcagcct
    12541 catggcatgg atagaatatt ttaagtcaac ggcacaagat cagaatcatg tggaagaact
    12601 tgaaaaagat gttgcaagat taaataccat aaccgaacgt ttttcaaaaa taggatcagc
    12661 accggtactc cgaaaggaag atgtttatat ggttatagaa aacactattt cctatatacg
    12721 ttcacgcaca tcgagtaagg taatttttag tctcaacaaa gcacagccac atcaggttct
    12781 tgcgccatta aacattccac tatttgaatg ggtacttgaa aatcttttca aaaatgctgt
    12841 tgatgctatg gatggcgaag gaaaaattga tgttatgatt accgatcagt tgcagtttgt
    12901 atatatagat attgccgata gtggaaaagg tattcccaaa tcaaaattta aaacagtttt
    12961 taagcccggt tacacttcta aaacccgtgg ttggggatta gggctttcat tgagtaaaag
    13021 aattgttgaa gagtatcata acggacacat ttttgtaaaa aattctgaac ctaataatgg
    13081 aaccactttc cgtattgtcc taaaaaaaca ggtttagccc aaggctattg ataatacctt
    13141 tcgcggataa tattaaaatg atgacgttga tgtacaatgc agaaatagag catgtgcctt
    13201 acagaatatt ttgtttgatt agcagttcct gttttatcaa aatcggcagc gtcaagcgaa
    13261 ttaaataatg ctaacgaagc ttcgcgcaca ataagatatt caatgccaat gctgtgcatt
    13321 gctcttgtct taaaatttcc gtttgccaca tattcgtttt catcaaaacc cggcaactct
    13381 gtcttgtcat tacgtgcaaa acataatgca cgataggtta aaattctttc tgtatcacac
    13441 agatgaccaa taacttcttt cagcatccat tttccgggtg cgtaactaaa tgtttcttta
    13501 ttgacaggaa tttgctccag catactttcc atttcatcag tagctgtttt tagtgtgagc
    13561 agcatgtctt cttccggaag atattttaag tagccttgat aatattccga aagttgttgc
    13621 ggtgatggtg gtttgataat catatctgat aatctttggg aataaaatta atactatttc
    13681 tgttaatatt atatttgatt caaatttatt ttaaaaatca tgagtggaaa tctattgctc
    13741 cgatatgcca attataataa atgggctaac caaagaatta ccaatgccat agtgagtgca
    13801 ggagaaactg ttgcagatac agaaatgaaa agcagtttca ataccgtgcg taaaacaatt
    13861 tatcatatct atgactcgca gcatacctgg attatgcgca ttcaaaacaa accatattcc
    13921 tggcctccga gcaaagattt taaaggcgac ttaaatgagt tttcaaaact gcttattgaa
    13981 agttctcagg aatggattaa ctacatttct aatctgaatg atgaggattt taaaaaaatc
    14041 atatcctata agaatactaa ggggatagat catcaaacac ctttggaaga aataatcatg
    14101 cactgtttta atcacagcac ctaccaccgt ggtcagttgg ttaccatgct tcggcaggcc
    14161 ggagtaaact cttttgagcc tatggacttt attgcttttg tgcgataaat attttgcaaa
    14221 cccgatctac tgtttcctca acgggaataa aacgataatt gagttgttca gtgattttac
    14281 tgttatcgta attgtaaatc tgctgtgatg tatatgcagt ttcattggtg attaaagacg
    14341 gttttcctgt aaaaacagat ttaagccact ctaacctcca ggccaattgg gaaagaaaaa
    14401 ctcctgcttt tatggttggt ggttttatgt ttaaaccctt tgccattgtc ttaaacaacc
    14461 attcgtaaga tttattctct gatgatatga taaaacgctc acctgatatt tcactttctg
    14521 tcaaacttat caggctacgg ctaacatctt tcacatcaac aaaacctgaa acgccttgag
    14581 tatagaactt cagtccgctg tacaaggtgt taaacaatga tgatgaatcg gaccgccagt
    14641 taccatcacc taatattaca gacggattaa taatgacagc atttaatcct tcagttatgc
    14701 ctcgccacac ttctctttct gcagcatatt tacttatagc gtaatagctg tttttttcat
    14761 tctgattaaa ccaatcgttt tcattgatca tctgttgtgt gccgcgacct aatgttgcaa
    14821 cagaacttac aaagcccaat ttaatatttc ttttttccaa acaggcattt accacattgg
    14881 cagttccgtc agcattgact ttatacattt ctttccgctt ttgcggatta aaagacacca
    14941 ttgctgcaca gtgaaacaca tgcgtgcagt cctgcagcgc atcttctata ctgaaaatat
    15001 ccaacacatc accataagca atttgtattt tatcaaaaag ctcaggcgat gacttaaact
    15061 tttttttgat taacactgtg gacgatgact tacgcaacaa aatcctaaca gatctgccca
    15121 tggaaatcag atcaaagaca agttgtgaac caaccaagcc ggaagcacct gtaatgagat
    15181 acataatcag atggtggcta taactttcaa ttcaatagct attggtgttg gcagacaatt
    15241 tatctcaact gttgtacgac acggaggatt ctctttaaaa tattctgccc atagtttatt
    15301 gtaaatctga aaatcatctt tcatgttggt aagaaaaaca gttacatcaa taattttatc
    15361 ccaactgctg cctgagtctt ccagaatgta tcttacattt ttaaatactg catgacattg
    15421 ttcagctata tcatattgta caatgttacc tgaggcatcc tgctccacac cgggaatttt
    15481 tttgctgccc ttttctcgcg gacctacacc tgataaaaac aataaatttc ctacacgtct
    15541 tgcatgtgga taaagcccta caggctccgg tgctttccct gaatcaattc gttgttgttc
    15601 tgtcattgtt ttatttcttt aatgttccaa tttatttcaa tatcccagtt agctcgtaat
    15661 gttagtgttt ctttatagta atatacaagc tcaggcaatt gttttaaaag ataatttcct
    15721 gttgtaaaag ttttatttcc atcggcatca ttaataatac gaatcttgca ttttgaagga
    15781 agcattcttt caaataaata acttccatta ccacctatat atttttctga aatgaccatg
    15841 tctttttcgt caaccacctg cagtatatag tttgttcccg ataaaccatc tactttaatt
    15901 tttacactgc catagtcggt aaattcattg gtatgaaaat ttagtttggt tgtgtcattt
    15961 ttgttgccgt aaatatctgt taccgctccc ggctgtaaag ttaaattcca attgctcttt
    16021 tcaatagttc ctgctttaat tgtaatgaat gatgatgcag aatcaaaaaa agcaatttca
    16081 aaaggttgtg ctgtagtgct atcacgcttc aacaatactt tttctttgtt aactgaagca
    16141 acaggtatag ttgttttaag taacacatca ttaccgggat aaatatttgt acttgaaagc
    16201 aagctgatgg tttgttttaa gtgccctctg agtgcttttg gcggttttga tattgtcaat
    16261 gtatcagtcc aattattttc gcttgatagt ttataactta acgtatcgct gatggaatct
    16321 ttcaaccaca caaccaaggt gtcattagtt ttactgcggt ttgttaattt gatattgtct
    16381 gctgtctgaa gtggttcaat atttaatacg ccaacaggtc tgttcatcaa aacactaaat
    16441 gcaaaagcgt taattgtctt tgattcttta acgtaaactt tcttatcggc ttctttaaac
    16501 attcttaaat ttacaggtgt agagtcgcct ccgcttacag gagttttatc aaaggcaatc
    16561 cattcatcag actgattgaa tttatagttg ttgtcagcat cctttaatgc aaagatttta
    16621 tacggacctt gtccaatatt ttttattgaa aaatttccgc tttcatcagt cctggtaaaa
    16681 taagaaggtg gattgttata gggcagcgaa tcatcgtcca atgtataaag cattacgaga
    16741 ataccttttt cactttttag attctccgca aacactacat ttcctttaac aaaaagtgta
    16801 tcaacatagg tgcctgtaga aaaaacgtat ctgaatcctg ccaatggatt cgactcatgc
    16861 aaatcggcaa cggcattgcc gaagttaatg gtataagtag tattttcttt taactttttc
    16921 ttaaaagtaa ttgtcagctt tttaccttta acttcaaatg ttggtgcagg ctcgataata
    16981 ggcgaaatga ctaactgctt gtctttgtca agtaactgaa tgtactcatc aaaagtaaac
    17041 actactcttt caccattaaa atttactgtt tgctcaggag ggactaccac tttgagcttt
    17101 ggaggtgttt catccttttt tcctcccgta ggtgctacaa cagaagcaca tgcacctaac
    17161 attaggagta cggcagtcca aagaaaaata cttttaaaag aaattatttt attcagcggc
    17221 ataggaaaat gtttctgaat aaagtgagct tattttttcc aggtaatact tgtctgtttc
    17281 atcaaaatga ttcaggtgtt cgctgtcaat gtcaataaca gcaacaacat catgcttcaa
    17341 aacaggaact actatttcgg agcggctcag cgagctgcat gcaatgtgtc catcaaaatt
    17401 atttacatca ggcactacaa tagttttttt ctgttgccat gctgcaccac acactccttt
    17461 tcccaattca atgcgtgtac atgccacagg cccctgaaaa ggacctaaca ccaactgttg
    17521 ttttccttca tgttttttca caaaataaaa tcctacccaa aaaaaaccaa aaacctgctt
    17581 gagcatggca catatattgg cagcattggc aacagcatct acctctccct taatcaatgc
    17641 ttctgcctgt ggtaaaagct gtgcatactt ttcttgtttg cttagtgtag tatctataac
    17701 tatggcctct gacattccta aaaattccgt gcaaatatca gaaattcaac gatgaggagc
    17761 agcaacagaa gccatggata ttttacaacc attaatacct gaaaaagctt ctgcacatga
    17821 aatgatggtt gagcctgttg agattacatc gtcaaccaat aaaacatgtt tgttttcata
    17881 aggctttaca tcgtcaagtt caaatacact ttctgtattt tcaaatcttt catagcgtgc
    17941 tttatgtgtt tgcgactgcg tgtatttttt tcgtttcaag gttgctgtat gaacttcttt
    18001 ctgccaattt tcagctattc ctaaggcaat catctcactt tgattgtagc ctctctgcaa
    18061 aagttttccg gggtgaaggg ggacaggaat aataatatct gcattgctga atgctgaagc
    18121 ctgaaacaac tcaatagcca tttgtctgcc taacatgatg ccaatttctt tacatccatt
    18181 gtattttagc tgatgtaaaa gtctttgcag cacctgacct ttctgaaaaa aataaactgc
    18241 tgtgacggct acaaaatcag tcttgcccca gaaatttttt gcaataggat tttccgggtc
    18301 attatgatat tgcgttttat gaagcccgta taaacattgc aggcaaacat gcttttcgcc
    18361 tctcaaaaag ctttgtccac agcccggaca gttgtcgggg aagaacaatt taaagaaatc
    18421 ttttatcact tgtggcggtt ttaaatcaag aagtaaatat atgcttttgt ttaaaaaatg
    18481 taaggtctgc cgaataaaaa agtgaaaatg agcggtggcc aaagaaactc caaaatgcca
    18541 ttttacgtag aaacacacaa tatttcatcc tttttacttg cttataaaat ctaattacat
    18601 tattttgcac cgtttaacaa cccatttcag atagatggaa gaatcagtga atgaacatac
    18661 caaagcagat aaccgtgagc aaaactcacg cagagcactc gtatttattg taatcaccat
    18721 attgttgggg gttaacggat tgttgttgta tcagtttttt gataaaaaga gtcatttgga
    18781 agaggtgacc cgtactcttg acaatacttc tgccgagcgt gatgccttgt cagcagagtt
    18841 gcaacaggta aaagcagagt ttgaaaaaat aaatcaggag aactcaagtc tgcaggctca
    18901 gttgcttagc aaggacgaag agattaaaag caaaatggcg caaatcagaa ggatgattga
    18961 gtcgggtgat gctacacagt tgaaggttgc acgagaagaa ttagaacgac tgagatcaat
    19021 gaatcaggtg tatgttgcac agttagactc catgcgaaat gccaatgttg cattagcttc
    19081 acaaaatgag tcgctgaatg acatgattac agtagaaaaa aataaatcta ctgcattagc
    19141 tcttgaaaat tcaacgctga caaaacgagt tgctgccgca tcagtactca ggactacttc
    19201 cattaaagca tcaggagtaa aatataagag cagtggtaag gagttagaaa ccaacaaagc
    19261 ctcttccaca caaaagataa aaacctgttt tacgatactt gaaaacaccg taaccacatc
    19321 aggatcaaaa gatatttata ttaagattat gagccctgat ggtgtggtga tgaccaataa
    19381 cactgaaaca ttcactattg ccggtcagac ttcgttgtat tctttaaaag agtcgtttga
    19441 ctatgagaac cgcgaaatga atatgtgtgt ttactgggaa aaaggaactg ccttcagccc
    19501 cggaaaatat actgttgagg cctattgcga aggaaacctg attgcttcaa ctccggtggt
    19561 tctgaaataa cttttcatca gaaaattgct cttgcctgca cattggcagt atgaataatt
    19621 ttatcgccct gcggcttgcg tccgttataa ccaatattga tttgcaggtt gcctgtaaga
    19681 tttttctgaa ggcttatttc ccatgtataa tttcttcctt tcttaagacc ttcaagcatt
    19741 tcaaaagcta atggcgaatt gacatcagca ttgtatccaa tactgattat attcactcct
    19801 aaagaggcaa caccattttt aaagctgctg tatttgcact ctgcacctat ggtagtgatt
    19861 gttgcatgtt cattagccaa ttcaattaca ttttgtttgt cctgatactt gtagatgagt
    19921 gtagttctga aaactgttcc cggttgcaca ctaaagcgtg gctcgatggc atatcctgca
    19981 atttcataat ttctatttgt aaaaaccgaa ctgttttgct tggtttcata tttcaacgct
    20041 gttgtgacac catacactct tttaaaatat gtacgtgtgt ttaacaacca tgaagtcagg
    20101 tttctgcctt ctgtgccgtt ggccagtaaa cttttattgc tgatattatc gtaattaaaa
    20161 tcggcagcaa acaccgatga actgcgatta aaaaataatg tgtttcgcaa agtagagttg
    20221 gtgatgagta aagatgtgtc gttaatgttg taaccgaaag gattcagtcc gtctgcccat
    20281 tttccggaaa gcagtttgtt gtcaaatcgt aatgccgact gcaaggcaaa ccggtttatc
    20341 agtttggttt taccctgttt gttttttatg aatgccgaag gattgacacc aatcacttca
    20401 cttagctgat tggtttgaat tctgacatag tcgtttgttg gtgtaaatat ttttatatac
    20461 tctgctctat cctgaaatgc agcaacttca aattcattca gttcggcaat accattaccg
    20521 ttatagtcgt tccattcaaa aacaccggca cctgctgcaa ctttaataaa ggaataagcc
    20581 tgtctttgtt cctgtccggc acctacctca taataggtat tgcttgttat tccaccattt
    20641 aaaaactgca atgctatttc taatttacca agaaagcttt cgtccttttt ttgtgtcgtt
    20701 atttcttttc tgtttaccag aatggttctg tacgtactgc ctaaagtaat tctctttcct
    20761 ctgcctgcaa taaatgcaac atcagcatcg gcctggtcgg cagtagtggc aagtttaaga
    20821 ttattatcac taggccgata gtcccatctg cgtgatgctt ctgctttata cgaccattta
    20881 cccgtatcag ccgatgtgaa ataaattcct gctttgtcat gaccaaaact gtttgacatc
    20941 aaactgtcaa ttcccgccac attcatttta ttacgctcat tctcttgcct gtaccctact
    21001 ttaagtttat taatactttt tccaccatca acatagcttc ttaaaaaaac agtgtttgac
    21061 tgtgcgcctg ttgtattcag taaactgcca ttatattgaa taaaatattt attccagttg
    21121 taattggagc tgatcatatt cattaaacct ttgtactgat ttccttttgt aaaatatttc
    21181 agttgataaa aaatattctg tgcaacattc ttttctaatc cggtattaat ggataagaaa
    21241 gtttcattca atgtgtcaaa agcagaggtc aggttccagt cacgaacaaa ctcaacatct
    21301 ctgtaatttt ccagtggtct gaatcttcta tccgttaatt cagcagaaac tacagagacc
    21361 catttccatg aactatcttg tttagaaaca ggcattgaat gatttacagc ggcatgtaat
    21421 gcatatccca catcgttaga tttatctttt cgggaaaaca gattaacatc atttttactg
    21481 aaggcgcctt caacagaaac gttcgtgttt ttactgagag caacgtctcc tcctgctgtc
    21541 aataattgtt gtcgtcttgg tgctatcaac aatctcaccg gttcgtagtg gccctgtggc
    21601 tgaccattta ccggagcaat ccattcatat acttttccat ttacattgct atttagcaaa
    21661 cggtaattac ctctgccttg ccctacattg ctgaatccta accgatagaa cgcccttgtt
    21721 gaatctatgg aataggtaaa ataaatgtat tgaatactat caattgttat agaatctttt
    21781 tgatataaaa cttcattgcg gttaaaccca acgctgtcgg cattgaggaa gtaagcattc
    21841 tgcaaattgt caccggcatt ggctaacact tgtcttttat cctcattcaa atcctgaaat
    21901 aatggttggt ttttattatc ctgctccgta aaatagttga catgaaactt tgcccacttt
    21961 gactcaaaga tgttagagga atacagaagt gtgcgcacat agtttttatc tgagtattga
    22021 aactctgcta ctattcttga gtctttgttg ataagtcgtc ttgcagaaaa tgttatttgt
    22081 gcagtgttgt aatcaatcac atagtcgttt tccttgcctc ttgtcagcaa aataccattg
    22141 agataaattt tttctgtgcc acttaaaaca ataatgtagg tttcattctc agcccccgta
    22201 agtctgtatg ggccttgatt ggcttccata ccattaaaca cattacgagc aaattttcct
    22261 tttgaaatag ctgcggcatc agtagttttc atcacccaca tacttttttg atcaataaaa
    22321 aatgacgagg acaccattgc accctgtgct tttttataca tattcataaa atatccgtaa
    22381 ggctttgaaa tctcaaaatc tccggcaatc aatcgtgtac tatcgcgcga aagctgaaca
    22441 aaaactttat cgaattcctg tagttgttgt gtgttcccct caggttgaat agggatattg
    22501 ttatcggtaa ttgcagcaag aatttctacc ctgtcgctta atgttcccga caattgaaga
    22561 tttaacccgg aattgacaat aacgtcctga ttgttgccaa aactgatacc tcttgaaata
    22621 cttccgcttt tgtttaaccc atcaaaattg aaaatacttt gtgagttgtt ttcagcgttg
    22681 tcataagcaa agggattgta ttgtcctgaa tatttctgtt taataacact caagtttcta
    22741 ttgctatgtg gcttactgaa attaaccgga aaaacacgat aagatattct taatgaatct
    22801 gaatttttct gcttgcacga tttattaata atcaaaatcc ccttttcgta tagtactttt
    22861 atgcacgata tatcccatgc aacggaatca attaaatgaa atgagccggg cacaatggaa
    22921 agtgaatcaa gttgcagtgt atccgagtta acacctatgg ttgcaattcg cagattactc
    22981 agcgactgtc catagccaag tgtaacactc agaaaaagaa aaaaaacaag cgacagccga
    23041 atcatacttt tgaagtgcta aaaatagcaa atgcaactgc ttgttaacga caagttgtgt
    23101 cactatattg aacagacagg cttaaatctt atcttgtatc tgtattcagt gcaactgcat
    23161 tctttttata gccaactatg cgttcagatt aatatccatt cacaaacttc atcagcgttc
    23221 gttttgccaa aggcagtttg gatttttgcc ggaattgaga gtgtatgccg ctgctaattt
    23281 gaattttact tcaacaacac tttgctttta tataccctga tattttcttt gtcgaataac
    23341 tgtagcgtgt aaattccttt agcaaattgt gagcaatcca gacttgaatg tgaattgagc
    23401 actgagcggt gaatttcacg tcctgttacc tcttttataa caattgtgag tggttgctcc
    23461 tgagtatagt aaatattaag tctgtcagtt gccggattgg gcgaaacggt aatgttttga
    23521 gctgcataag tgctgcttat tgccataagc gatttatctt gtttgactat tgttgatgac
    23581 aacactctca atcccgaact ttgctgtccg caaattaatg aagcatccat atattcttca
    23641 gcagttataa tactgtcaat caaatcttgc tccagatgtg ttatacaatt tttataattc
    23701 aggtaatcca attcatctga tgcagttgtc cagttaaaca tatcatccaa tatgggaaat
    23761 gccaaatctc tccttccggc caaacgataa acatttgcct tatccaagct gtaataaaac
    23821 cggtctaaag tatcgccatt gccgatggct tctgctaaat gtttattcaa cacctccatc
    23881 atttgctgca cttcactact tagtgcagga gtgttatcgg ttatggaaag ttcgttgttt
    23941 ttaaatgccg cgcccaatgc agttcccatt ttagaataac tgtaattcaa aatgtatttt
    24001 tctttatcat caggagttgg caaattatac atcagcattt cgttaaagag ggttactgct
    24061 tgtgcatcac tgctgcctcc ggtttctgtg gtttgttgca atgcagctac tcctgcctcg
    24121 ttaagtttat cgccattaaa gttagatgta taaatggttt cgcagttatt gcaatcatac
    24181 aatggatttt caggagcggc taccaagggt tgtgtgttac tgcaagatac aaatggtatt
    24241 ggattagggt cgttgatggt gacaggtatg gctgttgcac agcctgactg acctgctact
    24301 gtagttaatg cataatcgtt ggtagaaaag gttccgttgg tattccattc attataaatt
    24361 gcattaatgg cagatggatt tggacaagtc tttttaatag ttcctctcac ggctttctgt
    24421 acaccgcttt gaataggagt gaggctatta taaccatcgg ctaaaaataa attttttgct
    24481 acggaaagtt ttaatgagat atcattgttt tgtatggcat tggaaggttt aagagtaggg
    24541 ctgcctgaaa aatcaagcgt tgccccctga tttatctcaa tgccgttggt ttgattattt
    24601 tttacgctgt tacaaattcc tttcaactgt gtgtttttaa tttgcattcc cacaatgtta
    24661 tcgtggatat aattgttttc ggcatacagt gatgatgtga ggctgccgtc aaaaaatacg
    24721 ccaatgccat tggtaagcgt tgtatatttt atttcattag aggtcaacaa gcatggcagt
    24781 gtcatattat cggcacttat tccaaagcct ctgttctgat taaatacaga gttgttaata
    24841 tcaaaacttc cgccatcggt aagtactcct actccgcaat tctgaaaagt agaatattca
    24901 actgtaaaat cgccattcaa attgcggata ccctgatagc cgtgcaaaaa agtagaatag
    24961 ctaacagcag cagcaccacc ataaagcata agacctttat gggtattgta gtttcctgtt
    25021 gtggaggtaa acactgtgcc ccacagcgtg gttttggtgg ttgcacccaa aacccttgca
    25081 gcactgtagc ctgtaaactg gcatacgcct ccttctacaa taaacttagc caagcccaca
    25141 ggcaaccgca ggctttcctg agtaactttt aaaagaagtt gacctgtgcc tccgactaat
    25201 tggaaaatgc aatcgcccgg tgtgcaggct acattagccg agggcgatag cggactgtta
    25261 aagagaagat accccaaacc ggagaaagta aaaatattat atgcagcagt aacaggtgat
    25321 tttaaatcga gcaagcccgc tatttcaagc gtgctgttag ttgttgccag tgtaatatca
    25381 gcctcctgat acgttaacgt gccgccatct tctataataa tcctgccacc gttgtttata
    25441 acggtgcttt ttgaatcaag aattaatttg cagccgtttt ttactctcac gattgagcct
    25501 gactccatgc ttaactggct gccggtttca agatgcaaaa cactattgtt ttcaagaatt
    25561 aattgtgagt tggattctat gtggaaatat gatccgcctt cacacgtaaa agttgttatg
    25621 ggggtaaact cacctgaaac agcatcgcga tttatctgat ttgctgtacg gctttggtca
    25681 agggtgataa ttttatttgc ttttaaattt acttgctctt tcaaaataat atcaccacac
    25741 cagcgaacat catttttaac atcatactca ttccatacaa catcaacctg aaaatctcct
    25801 cctgttagtt gcgtaaaaga aatttttaaa cctgtaaggt atgtatgtct gttattagga
    25861 gcatttgttt gtgggtaaaa agtactgccg gataaggcat aataaaaagt aggcaagtta
    25921 actggtgaag gattactacc aatttccata acagttccat ttgtaaatgc atctttatta
    25981 tcgccaaggt atggtatgtt actatcaaaa ctattgttta gcctttgctg aataaacggt
    26041 gcatgaccat tggaattatt tatagtattt gttaaggtat tataaaagat atgcatttga
    26101 tcattatctc cggttaaagt atttgattct aatagatgat agctttctga agttggccag
    26161 gcaacgcagg atatggattg gtttgcaatt tgctcatggt caaaatttcc ttctgcgctt
    26221 attaccttta aattatctgc ctcaccggaa ggatatacat ctaattgatt gttggactcc
    26281 agtatatttt ttccaatttg catgtaagca tatatgccgg gtgtgccttg tggccggcat
    26341 gggtcgctat cgctaaattg taaataatcc agcattccat tgctacctat tttatgattt
    26401 tcgagccata tatattggtc aagtgcattc acatctttat agggcagttt tattctaacc
    26461 acatctccgg ttgtcataaa atcacgaagt acaaaagttt gtgctcccat ggactggtca
    26521 atatccgaag ctgttccgtt tgcctgaatc ggaatttgat tagggttata agttgaactg
    26581 taccaaccca tgcgatagcg ttcataaccg ttaacagaag ttaaacccgc tcctgcactg
    26641 cccattaaac catacccacc ttgtatgcca agaaatgttg tacttggttg acttccgtaa
    26701 tgatgcccgc ctgaggtatg aaaattattg cccccaaaaa gattatgagc taactcatgt
    26761 ataataatac cattggggtt ggtaagtgga ctttttccca cagcctgaac agaaataact
    26821 tctcctgtat agaaattact tccgattaat atatgtgatg aaagcccgcc tccagtatag
    26881 ccttgcccta attttaagcc accataattt gacgtactat tacggaataa gatctgaaca
    26941 aaatcaatca tattattaga ggttaaattt ttagaaatac ccggaaatcc taaagcacaa
    27001 taatcataat cattaattga attatgccca aaaattgcgc ttaaacctcc tgatgcatta
    27061 attatattaa ttgcattttg taataaagta tatgaagaaa ataaatacaa tgggggttgt
    27121 attccactat tatttattat ataagaatga ggcacatcta caactataaa atctccaact
    27181 atatgcaaag ccccaaaact ggactcgtag tattttcttg tcattattcc ctgtggcggc
    27241 agggtattaa aatcttgatc catgaaagaa gataagtagg caggaggatt actgtttatt
    27301 gtattagggg tacccggatt ccagtcatta gctcccggag ttgcttggta ataaggatcc
    27361 catgaagggt taacatcgta aataatgtta atcataatgt ttaaaaccct gtaattcgtg
    27421 tgtggaaaaa gcgagaatcc gtttttacta caataagtaa aattgctatt gcataatgtt
    27481 gctgatatat ttccaatagt gcgtagtgca gtagaatctt caatatattc acattgcgaa
    27541 aaggaattaa gactacttaa aagcaataaa gttgagataa ataattttct tttcatattt
    27601 ttatgatttt aaaacttaca gtttctgttt tattaaataa gataagagtg taaatcccat
    27661 ccttaatatc caatgcaggt atttgcttat tttgtaactt acccccttta attattatac
    27721 cctctttgtt tattattcta taataaaaat ccttgttatc tttaaattca atatttataa
    27781 tattaaaaaa cggcaacgga taaattttat aatccgaatt ttgattatta aactcttcta
    27841 aaccaacaat tatatgttga ggtataaact tgtaataaaa tatatcaggg gcatctaatg
    27901 tatcaacgag cgaaggatta atatatgcat cataggggta ataaccaata tgcgtttgga
    27961 cagaatcccg ccaagttcgt acccagccat tgggattacg gttgtgagaa ttaggattga
    28021 atattggatt atatgaacaa aagcaccctt tgtcagcaga atccggcaaa aaaatactgt
    28081 ctctgaatat gctgatttta gctattatgg gtgttgtgta ataataaaac ttaatggtat
    28141 aggcaagagg gtcattaaaa aaattataaa atacaccata taaagaagtg gaatcacaat
    28201 accttatatc atactttgtg taccagtcat aagcaaagcc atcactcatt ttgttctca
//
LOCUS       DAOQNP010000032        28074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_547_length_28074_cov_8.951497, whole genome shotgun sequence.
ACCESSION   DAOQNP010000032 DAOQNP010000000
VERSION     DAOQNP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 28074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_28074_cov_8.951497"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..272)
                     /gene="atpC"
                     /locus_tag="PKY09_06110"
     CDS             complement(33..272)
                     /gene="atpC"
                     /locus_tag="PKY09_06110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNB12467.1"
                     /translation="MFLEIITPDKKVFEGEVTSVSVPGADGKFQMLNNHAPIISTLGK
                     GKVKVKTAKETLEFNVKGGVVEMLKNKVVVLAESV"
     gene            complement(350..1852)
                     /gene="atpD"
                     /locus_tag="PKY09_06115"
     CDS             complement(350..1852)
                     /gene="atpD"
                     /locus_tag="PKY09_06115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNB12468.1"
                     /translation="MNKGKITQVIGPVVDVSFEGENTKLPNIMEALEIKRPDGTSVIL
                     ECQQHIGEDTVRAISMDATDGLVRGMEVVATGDAIKMPTGDAIKGRLFNVVGDAVDGM
                     KAVSKDKGLPIHREAPKFEDLSTASEVLFTGIKVIDLLEPYSKGGKIGLFGGAGVGKT
                     VLIMELVNNIAKAYSGLSVFAGVGERTREGNDLLREFIESGVINYGDDFKHSMEQGGW
                     DLSKVDYNKLTDSKATLVFGQMNEPPGARARVALSGLTVAEHFRDGDGTGSGRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKRGSITSVQAVYV
                     PADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILTEEVVGKEHYET
                     AQKVKQLLQRYKELQDIIAILGMDELSDEDKMAVHRARRVQRFLSQPFHVAEQFTGLK
                     GVLVPIEETIKGFNMIMNGEVDEYPEAAFNLVGTIDDAIEKGKKLLAEAK"
     gene            complement(2058..2924)
                     /locus_tag="PKY09_06120"
     CDS             complement(2058..2924)
                     /locus_tag="PKY09_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNB12469.1"
                     /translation="MRQLKITNMITIRENECLKRYLNEISQENLLTPNEEDQLARRIK
                     TGDQQAMNKLIKSNLRFVVSVAKQYQNQGLTLEDLINEGNIGLIKAAQRFDSTKGFKF
                     ISYAVWWIRQTIMIALVEKVRLVRLPFNQVSQFSRIKKLQSEFEQTHGREMSLLELAE
                     AMKTSPQAVKEAMTVNMFPVSLDSPVSNDDESSSMHELYSDPSNQPVDFNLSNESVKH
                     DLMITLKQLKPRERQVVKMYFGIECDDQYSVEQIADRLDITRERVRQVKDKAIKKLKN
                     KKTADILKAHLC"
     gene            3252..3333
                     /locus_tag="PKY09_06125"
     tRNA            3252..3333
                     /locus_tag="PKY09_06125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3286..3288,aa:Leu,seq:tag)
     gene            3393..4742
                     /gene="tig"
                     /locus_tag="PKY09_06130"
     CDS             3393..4742
                     /gene="tig"
                     /locus_tag="PKY09_06130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNB12470.1"
                     /translation="MNIQKEDVAPLNSLIRIKLTPEDYKTDVDSAIKKFKKSASMPGF
                     RPGHVPEGMIKKMYGKSFLADEINKIVSKSLYQFFRDNNIAVLGEPLPKEVENQNNFE
                     NPSDFEFLFEIGQAPVINLDLPSLPAVHYYQIEVDEKRVDTYIEDLQRKHGNFSNPET
                     SEESSILYGEFIELNENEEEKENGIRSTTTLAINLVKDETSKAKLIGLKKEDEVTLDL
                     MKAMQDVTEVAYMLRVEKETAQQITSLFKYKIASINKVEKIEVGQELFDKVFGENAVT
                     SVDDFRSKVKEDIAAMFARESMHKFNHDVEDALLHHLNISLPDDFLKRWIMEVNEKPL
                     TPEQIEQDYPAYSRQMKWKMIEGKLINDLQLTADEHEMAEYATAVIMQQFGQYQLGPD
                     MIADFAKRYLQTEENRNKADEAVKSRKVFDYLNQNVSKDVKVVSYDEFVTIVKEHKH"
     gene            4765..5463
                     /gene="clpP"
                     /locus_tag="PKY09_06135"
     CDS             4765..5463
                     /gene="clpP"
                     /locus_tag="PKY09_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNB12471.1"
                     /translation="MNFKNEFFKYATRHHGINSTAVGHYIESAVRAEGYISPTIIEER
                     QMNVASMDVFSRLMMDRIIFLGVPINDYVANIVQAQLLFLESTDAKKDIQIYMNSPGG
                     SVYAGLGIYDTMQYINPDVATICTGMAASMAAVLLCAGAKGKRTALKHARVMIHQPMG
                     GAEGQASDIEITAREIQKLKKELYEIIASHSGKDYDTVWKDSDRDYWMTAGEAKDYGM
                     IDEVLVRNKATTDK"
     gene            5484..6722
                     /gene="clpX"
                     /locus_tag="PKY09_06140"
     CDS             5484..6722
                     /gene="clpX"
                     /locus_tag="PKY09_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNB12472.1"
                     /translation="MKKQNEVKCSFCGRDKSATQVLIAGLNGHICDQCVTQAVEIISQ
                     ESADQQKSKISSSFTLLKPAEIKKHLDDYVIGQDYAKKVMSVAVYNHFKRISGVGKKD
                     NDVEIEKSNIIMVGETGTGKTLLARTIAKILKVPFCIADATVLTEAGYVGEDVESVLT
                     RLLQAADYNVAAAEHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKMLENTIIN
                     VPPQGGRKHPDQKMIPVNTQNILFICGGAFDGIEKIIERRLQSQSIGYSAHRNQDIFD
                     KGNLYQYVSPADFKKYGLIPELIGRLPAISYLQPLDRAALRSILTEPKNALIKQYQKL
                     FEPEGIQLKFDDNVLDFIVDKAIEFKLGARGLRSICETIMLDLMYELPQVKNKKEHTV
                     TLEYAREKIDKSNLGNLKVA"
     gene            complement(6821..7072)
                     /gene="purS"
                     /locus_tag="PKY09_06145"
     CDS             complement(6821..7072)
                     /gene="purS"
                     /locus_tag="PKY09_06145"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNB12473.1"
                     /translation="MKFLAHIKVMPHKALLDPQGKTVSASMHNINLPMITNVRIGKHI
                     TCEVEATDKKDAQAKVDEACKKLLSNPIMEFYEFELTEA"
     gene            complement(7082..7843)
                     /locus_tag="PKY09_06150"
     CDS             complement(7082..7843)
                     /locus_tag="PKY09_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNB12474.1"
                     /translation="MSLKQHIPNFITCLNLLSGCVGIVFCFTGHITGAGWMILLAAVF
                     DFLDGFAARSLKAYSAIGKDLDSLADVVTFGVLPAILMMQLFIVVNPQQHTNAAMPFP
                     ELILPWLPLLLAVFSAVRLAKFNNDTRQTNSFIGLPTPANALAIAAFPTLISNYLVNS
                     TTQNNNWMEMAVYMLFQHPVAYIIFILTMSWLLIAEIPLFSLKFKNFSFRENFFPYLL
                     LASAAILLLVFKIGALPLIVILYVLLSVIKNSTTT"
     gene            8021..9094
                     /locus_tag="PKY09_06155"
     CDS             8021..9094
                     /locus_tag="PKY09_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNB12475.1"
                     /translation="MRKITLSWFFIIACFASLAQTAPKYSNEFLNLGVGGRALGMGGA
                     QVSQVNDVTAGYWNPAGLVRSRGDIQLAFMHNEYFAGIAKYDYLALTAPIDTMRHIGF
                     SVLRFGIDDIANTIDLKDNDGNIDYNRVTGFSAADYAFLVSYASKSAINGLDYGANFK
                     IIHRKVGDFAKAWGFGLDAGIQYQAGNWRTGAMLKDVTSTFNAWSYTLNDRTKEVFIQ
                     TGNEIPENGLELTLPRLILGVSRQLNIRKFTITPALDLETTFDGKRNTLVKTNFASID
                     PRVGVEGGYNNLVYVRAGVSNFQQVTNIDGKKTTTVQPNIGVGIKLRNLAIDYALTNI
                     GNNNYLYSNVFSLRLTVNKLKKT"
     gene            9091..14154
                     /locus_tag="PKY09_06160"
     CDS             9091..14154
                     /locus_tag="PKY09_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNB12476.1"
                     /translation="MRKLYAIIILFLLPVLSFAQFSNSWINFNGASPYSAQQYFKIKV
                     WKQGIYRLDYNLLQQAGFPVGMNPNQFQIFNNGHEQYIYVEGAQDNSFDPSDFIEFYG
                     KPNNGSADQELYDNPADQLNPDYSLFCDTAVYFLTAAILPGTPVKRMIMETDVNFSLY
                     LPENYITKTFKRNYTSKYLKGDSQGDIDPSYNGPEGFSGNTFATATPADNFSLPNVST
                     FATAPIPSADVRMVNANYDGHFHTFNVEFNGNTIFTDNTIFGYGRHDYNFNIPIASLP
                     LSNSVVFSGLANSGGTNLMSVTYFKLQYPHANSFNGELEPFQFFSVSNGGSKARVDFT
                     DFLNSDNSTRFIYVISGDTVSKVTTVRTGNLLQALIPVNGGEKNCLLTPVSAHYSSVA
                     DFYLSPENSDPDPQKFARFINYSYNQPQYDYLIVSHKKLWSEAENYRNFRTQTGYNPL
                     LVDIDELYDQFAFGIYKHPLAIKNFCRFAITAFNNKPDYLLLLGKAVYPEEVRVNPTA
                     DQLCLVPTYGNPPSDFMFSNKIVDTTYVPRLATGRIAAQNVTDVTNYLEKAQAHEAAR
                     QNCPDDWLKHVLHFIGGNNGFEQQSIKAFMDQNEAAIRDTSMGCTVTNYYKISTDPIE
                     YIQAIELQARIDSGVSIMTFFGHAAGSTFDIATDIPQNWHNTGRYPVVFAQSCNVGDI
                     YSPSRLLNEDFVLLPQKGSVGFLAKPRLGNIQELGEYSVQLYRNVAYRDYDKPIGMAI
                     KHALDSMIQTYYANNGMVYKSTALGMLWHGDPGLRLAPSLQPDYAVSQPSITFQPTEV
                     TSELTDFKMNIAISNFGKATKDSVSVHVVRKFPDNSTVVIDTLIPYIPYKDTLQLTMN
                     VEVNKGTGLNYFDVFVDYAGTITECNEFNNRVLNVPLQIISTDITPVYPFKYAIVPDP
                     SVILKATTDNIYAAQKTYRFELDTTDAFNSPFLRFKTFTQTGGIVKWPLPFTCDSGRV
                     YYWRVSLDPSLYPTTFKWKESSFIYIPQKTGWSQAHFFQFKNDSYTNIVYDKPQRKWE
                     YENTQAELRVSAWDVPGQGITSDNVQLNNQLIATGGCNYRAIYAVVLDSISLQNWNTL
                     DLAGKFGNINQPPYYDSCQATVNGSQGFFAFATDSAYSVGGNTIYRNLDSLANFINRI
                     PTGNYVVLYSVTEHFMSKSWSGQFRFALSQQGINSVDTIPDTKHFIFFFKKGFPGTAQ
                     TVVAASDSTKNITFTTMLGGNWYKGYMMSEIIGPAIQWNSLHWANNSVEAINTDTLAL
                     DIVGVDTLNSEHVLYSAIQPSQIDFNLNINPFIYPRIYLRAYMEDSVYRTPPQLNRWQ
                     IYYQEVPEAVINPVGSSFQSSNVSQGDTIHWKMPVENVSLSDMSDLLVDYYLFDKDNV
                     RRNITSTRFHALLHGDTLNASVRFSTAAYPGLNSLWMEVNPRNDQPEQYHFNNYARID
                     FNVNRDVTNPILDVTFDGQHILNGDIVSAKPQILIKLKDENRYLALNDTSKFKVYVKN
                     PDGVESQIYFETTANSSLSENLLRWTPAQLPDNTFKIEYNPVFNVDGIYELRAQARDE
                     SGNLSGSNDFRISFEVINKSSITNVFNYPNPFTTRTQFVFTLTGSEVPTYFKIQIYNI
                     SGKVVKEITQQQLGNIHIGRNITDYAWDGKDEFGDQLAKGVYLYRVITQLDGDDIEHR
                     NTTADKYFRRGWGKMYLLK"
     gene            14174..15520
                     /locus_tag="PKY09_06165"
     CDS             14174..15520
                     /locus_tag="PKY09_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12477.1"
                     /translation="MKALAGYFSLWLKKNTFLLWALTVIHLIFFILALRNGNIAFSVD
                     STEYLNQAENIREHGTWYAGNSSAPHDVFLESRRPPLYGFFIFLVHIVSSNNFFLLAL
                     QNLMSIAVLILTAFMFWLLTGKRIANQWLVVLPLLLFPTQFIYANMIMADVLFQFLII
                     SGFLFFIQSVRKPNSFLYFCIFMSLAILTKPVFYAFCLIVPIIALLFYTKGIYKLQNV
                     WISLIPLLTVASISTINYSKTGYFHYSSVNEKFISEYGAYLAVGDKGNVNAQQKIDSL
                     LSEAKRQPDFKSYCKYINKESLYLIKENKARFVMMQLKGMANFFIDHGRFDLMAFFVH
                     PDYVQTQGWKQKYEEKGWQGVFSYLKTFNPFLLLFLILASIFNIIMVVILVRFLFSHK
                     IAILFRLLLLMFVIYMVVFTGVVGCSRYRMAIFPIIWMAFMWFLAERKSVHHKTLV"
     gene            15640..16131
                     /gene="cdd"
                     /locus_tag="PKY09_06170"
     CDS             15640..16131
                     /gene="cdd"
                     /locus_tag="PKY09_06170"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNB12478.1"
                     /translation="MQKIQLTSSYEEYNSLNELPAEYKSLMLEAHDAAKTAYAPYSAF
                     HVGAAVLLANGKIIKGSNQENAAYPSGLCAERVAVFASSVQYPGVKIKAIAVTAMPGN
                     KEVPVMASPCGACRQVMVEYEHLYHQDIKFIMYGSVNKIIVLDSIKALLPFSFNSDQL
                     SGK"
     gene            16172..17170
                     /gene="pdhA"
                     /locus_tag="PKY09_06175"
     CDS             16172..17170
                     /gene="pdhA"
                     /locus_tag="PKY09_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNB12479.1"
                     /translation="MAKQKFTKETYLFWYEQMLLMRRFEERAGQLYGMQKIKGFCHLY
                     IGQEAVVAGAMSVIKPSDNMITAYRDHAHALGKGTSARAVMAELFAKATGCSKGKGGS
                     MHLFDAANRFFGGHGIVGGQIPLGAGIAFAEQYKGTDSVTLCYMGDGAVRQGAFHEAL
                     NMAMTWKLPVVFIIENNNYAMGTSVERTSNVTDLYKLGTAFNMPSEPVDGMTVETVHE
                     AMEKAVNHCRKGNGPYLLEMNTYRYKGHSMSDPAKYRSKEELEKYKAQDPIEEVLKVI
                     QKNKLASQDEIDAIDKKVHDIVEDSVTFAEESPFPDADELYKDVYEQSDYPYIIED"
     gene            17199..18485
                     /locus_tag="PKY09_06180"
     CDS             17199..18485
                     /locus_tag="PKY09_06180"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HNB12480.1"
                     /translation="MAEIVRMPKMSDTMTEGVVAKWHKKIGDQIKSGELVAEIETDKA
                     TMEFESYQNGTLLYIGVEEGKAAPVDSILAILGKPGEDFKPLLDAESKAKPVTAAAPE
                     KTEAKPATKVTAEVPAKSVAAPKAESIPVAQNVVVSASDSRVKASPLARSLAGQKGLD
                     LSMIKGSGDNGRIVKRDVERFKQGNVVAGSYTSAIHTQESFEETPVSQMRKTIARRLS
                     ESKFTAPHFYLTLDIEMSRCMDAREAINAATGSKVSFNDFVIKACAAALQQHPKVNSS
                     WLGETIRTNHHIHIGVAVAVDEGLLVPVVRFADSKTLSQINAEVKGYAQKAKDKKLQP
                     ADWEGNTFTISNLGMFDIEEFTAIINPPDACILAVGAIKAVPVVKDGNVVAGNVMRVT
                     LSCDHRVVDGVTGAKFLQTVKGFLENPVLLLGQASI"
     gene            complement(18478..27201)
                     /locus_tag="PKY09_06185"
     CDS             complement(18478..27201)
                     /locus_tag="PKY09_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12481.1"
                     /translation="MPRIIAFCVLYFVANVTLAQDIFMSNGVMSGCSGTFFDSGGDST
                     NYSNNENYVYTICSDQPGKCVQLSFQSFAIEKDFDFLFIYNGPSTSSPLLGTFTGTNS
                     PGTITATSGCLTIRFSSDYTVNRGGWKAYINCINCPVNGCPTCNGGAPPVNDACSGAQ
                     NLGLLPLPAACPNGIGAVSLTNTSNLCATAEIPYQSLQGCKPIGSMSNPATDVWYKFS
                     LTAPVLDITINGLITPQVGLYSGTGCGNLVPRGCAIGGGGYLHTTFGGLAAGTYYLQV
                     SGGSINDQCNFSLSLQNNFDCSGCVMQSGLTVSPPPSNGFYLSGQQVTFCLNVTDFNP
                     TSANWLHGIVPKFGPGWDTTTIIALPPASCSNNGFWSWYSNPIISSANNKPYGPGFFY
                     ETSAGNSSNTQDNNPGNNFGDNLGQNCNLNFCWKINTLPQSLCNFGTNLNVFIETYTD
                     GESGSWTSAACANDPVHDLYATLACCIPPVVAITQPVCFGTQGSAIGTAQGSGPWTYI
                     WKNSSGVIIQQNGPVNGIDLISNLNPGTYQLITEDVNGCQSTVSFAVTQPQPLTATLT
                     VSATKCGLVNGSINVTASGGTAPYNYSINNGVSFQGSPQFSNLSSGNYNITIKDAHNC
                     TVSVPATINASTVPVINSFTWQNITCYNGADGEITVLASNGISPYVYEIKKSGLTLSQ
                     SSNIIQNVPAGNYQLTVTDVNGCKSDTTVILTQPPQINMVSVITPSGCNLNDGTIELT
                     ITSSSSGTLLYSIDNGTNYQTGNLFSNLSPGVYYVLVEDNNGCMIKDTAIVNTINAPN
                     IDSLKTQSLTCYRSQNGAINIFGSGGVGTLSYSINNGSTFYATNSFNSLTAKTYTVVI
                     KDNNNCRIYQLASVKEPPPIIIRGDIVATSCGFNNGSANLHPDNGVLPLEYSTDGGLT
                     WSTTNYYGQLPPGTITFTVRDANGCTSTRPYQIAASTSPSFDNVSVQSLKCFNTNTGS
                     ITLNAIDGIPPITYSIDSGATFQATNQFQNLISGNYYLTLKDSKGCRADTMLQLSQPF
                     PLSFTSAVTNTICSYPNGSIVITGNGGTTPYTYSVDGGATFTANNNNTNLIPGSYKIV
                     IRDTNNCEEASTVSIIDEPGPRITGHSFTPQICDNAANATVTINAISGTGTLFYSADS
                     GLTYSIANNINNLNGGSCTLIVRDANLCTDTLALHIPILHSPQIDNNTITNPICFGSN
                     DGAIQITASGGNGNLSYSIDNGSTFFANSTFNQLIAGTYYLTVEDSNYCQVKDTVILT
                     QPPLLTVQTLVNNETCSNQNGMVSILAGGGTPAYYFELSNNTTATDTFFTQLGAGNYS
                     IITTDFNGCTTQTAFQINNLDKPTINQAITSNLSCYQSNDGVIQLAAAGNGTLQYSLD
                     GIIFQNSNIFNQLSAGGYTITIRDTNGCTSDTTILLTEPNAIIANIQTTSSNCTLSNG
                     SLTVNASGGTGSLTYSLNGGAFSSQTIFNNLSSGNQNIIIQDANNCQTAFVASINTIN
                     GAVINNIASVDIKCHGQQTGEITIAASGGTGPLSYSIDNGQSSVSSNHFTMLAAGNYN
                     IVLTDSVGCITSSNITLNEPDSIIIQQAVTAATCNQNNGSFQIVVNGGTGTLQLSLDS
                     INFSPGTIFNNLTAGIYHLHVTDANQCYATKLISVPNQAAPVLTNLSKSDITCFGDST
                     GFISLSASGGTGLLNYSINNGVTYQALPNFTNLPAGSYHIKISDQNFCTADTIIQLTS
                     PDEITATLNITNANCQQSNGSITVNAQGGNGTLLFSLNGGPFSQQTYFGSLPAGTQQL
                     QVKDALMCNNNFSAQINTINGPTLDTVITQNLNCFGINSGTISILASGGSGVLSYSLN
                     NQAFINTANYNHLAAGTYQVSVTDSAGCLVSGLVQLTTPSAIQVTPTVSNPACGQNNG
                     SINLQLTGGTGMLLTSLNGSSYNNITNYSNLYAGSYSIKIKDANNCEVQRAVSLSNIL
                     APKLIGSVLKQVKCFGTNDGEIKILASGGTGSLTYSIDNGITTQISNTFSNLTAGTYS
                     ASVIDQNNCRSDTIITLLQPDSLQLLLNITPETCSAGNGSIAVSSTGGTSPYLFSSDS
                     GRTFFNVSGFTQLANSNYYIVVKDSKNCRTLDQVNIVNLTGPQIISLTTTNVSCYGLQ
                     DGEVQITTTGGNGVLSFSTNNFITQQADTFFNALLPGIYTVYVRDTNNCKSSHSFSIS
                     QPTSVQSNITSVQPTCFGYNNGSLNVVANGGTPPYNILWSSGSTGFGLSGIASGQYSF
                     TITDAHNCNKSDSIYLSSPTALAANHTTQNGTCSGSTNGSAMVAVTGGTPPYQYNWSP
                     IAGNTNYANALPAGLYTVDITDKKGCTLTEQIQISTPAPIANLFTKNDVSCYGGSNGM
                     LTATALGGNAPYSYLWSYQQQTNDTIQNLPVGSYTVTITDANGCDNSFTEQITSPQQI
                     KPNSTINNVSCNGFNDASVILTVQGGVAPYSYQWNNGVTQSLNTNLTAGIYVVTVTDA
                     NNCSRVKAFTITEPQPILLSINPVDTICIGQQATLNAIATGGTGAYNWLWNNQTAAST
                     ILVTPSVTTNYFVSVTDSNNCPSKTDSILVNVFPPLGIAATGNDTICAGQSAVLSSIA
                     NGGNGGPYFYLWSNGSDSSIVNVSPSVTTTYTVSITDNCTQIPATAMIPVVVNETPLL
                     DISPLTEKGCMPLEVQFLNNSTYPLGTIFTWNFGDGQTNDSITPTHLYNTDGIYTVSI
                     QAITPDNCVSNMIMPNLIEVYPLPVASFNISPESISILHPEINISDSSLLGSIWFYDF
                     GDGQTSTLQNPAYSYQDTGRFRVIQIVESEFGCCDTAKKEAIVEGAFTVYIPNAFTPN
                     NDGSNDYFFANGYGITELTMQIFNRWGNKVFETPSVAAKWNGIDTSGRICPQGVYVYT
                     AKIKDQFGIYHTYNGQVTLIR"
     gene            complement(27323..>28074)
                     /locus_tag="PKY09_06190"
     CDS             complement(27323..>28074)
                     /locus_tag="PKY09_06190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNB12482.1"
                     /translation="DTTYINAMKFIQIFSQLGLMLVPAWLCGWLFSDNISLFFQFKKI
                     NFLQIAVIVVVTICLSPLINLLLDWNSHLQLPASLAAMEKMMRNMETEAEKLTESFLV
                     THTNFDLIINLSMIALLPAVGEELLFRGVLQNLIQQATKNQHWAVIITGFIFSAIHMQ
                     FFGFLPRFMLGVFLGYLLVWTGSIWAPILAHFINNGSAVLLSYYEQKNAININEEKIG
                     IREGEFWMVIASVIITVSACYILYRSKQKST"
BASE COUNT         8672 a         4991 c         5707 g         8704 t
ORIGIN      
        1 gggcagcttt ttaccaatga ggaataattt tattaaacag actcagccaa aacaacaact
       61 ttgtttttaa gcatttctac cactccgcct tttacattaa attcaagagt ttctttagca
      121 gttttgactt ttaccttgcc tttgccaaga gtggaaataa ttggagcgtg gttgttaagc
      181 atttgaaatt taccgtcagc accgggaacg gatacagacg ttacttcgcc ttcaaacact
      241 tttttatctg gagtaatgat ttctaaaaac atttttaatt tgataattag ttaatctgaa
      301 aatttgaaaa tgaaaaacaa atttattctc atcttttcaa actgataatt tatttcgctt
      361 ctgccaataa tttctttcct ttttcaatag cgtcatctat tgttcccaca aggttgaatg
      421 cagcttcagg atattcgtcc acttcgccat tcataatcat attaaatcct ttgatggtct
      481 cttcaattgg cacaagtaca ccttttaatc cggtaaactg ctcagccacg tggaaaggtt
      541 gtgataagaa acgctgcacc cttctggcac ggtgaacagc cattttatct tcatcactca
      601 actcgtccat acctaagatg gcaataatat cttgcaattc tttataacgt tgcaatagtt
      661 gttttacttt ttgtgcagtt tcataatgtt ctttacccac aacttcttca gtaagaattc
      721 ttgaagtaga atccaatggg tcaacagcag ggtaaatacc aagctctgca attttacggc
      781 tcaataccgt tgtagcatca aggtgggcaa atgttgtagc cggagcaggg tcggtcaagt
      841 catcagcagg tacatatact gcctgtacgg aggtgataga tccacgcttg gtagaggtaa
      901 tacgctcttg catcagaccc atttctgtag ccaatgtagg ctgataacct acggccgaag
      961 gcatacgtcc taaaagtgca gatacctcag agcctgcctg tgtgaaacgg aaaatgttgt
     1021 caataaagaa aagaatatcg cggccactgc ctgtaccatc accatcacgg aagtgctctg
     1081 ctaccgttaa tcctgataat gcaacacgtg cacgtgctcc tggaggctca ttcatctgac
     1141 caaaaactaa ggttgccttt gagtcggtaa gtttgttata atctaccttg ctcaaatccc
     1201 agccgccttg ttccatgctg tgtttgaaat catcaccata gttaataacg cccgattcaa
     1261 tgaactcgcg cagcaagtca tttccttcac gtgtacgttc accaacaccg gcaaatacag
     1321 aaagtccgct ataggctttg gcaatgttgt ttaccaattc cataatcaat acggtttttc
     1381 ctacaccggc accaccaaac aaaccaattt ttccaccttt gctgtaaggc tccaaaaggt
     1441 caattacttt aataccggta aacaaaacct cagaggcagt agataggtct tcaaactttg
     1501 gtgcttcgcg gtgaataggt aagcctttat cttttgaaac ggctttcata ccgtcaacag
     1561 catcgccaac aacgttaaac aaacggcctt tgatggcatc gcccgtaggc attttaatag
     1621 catcgccagt ggcaactact tccattcccc ttactaaacc atcggtggca tccatagaga
     1681 tggcacgcac agtgtcttcg ccaatatgct gctggcactc taaaattaca cttgttccgt
     1741 ctgggcgttt aatttccaaa gcctccataa tgtttggaag tttggtgttt tccccttcaa
     1801 atgatacgtc aactacaggt ccgattacct gagttatttt acctttattc atgaattata
     1861 ctttttattc gttctgaaaa ctgatttttc aaggcgcgaa agtagcatta attttaattg
     1921 ctttgaaaaa tataaacgaa tgttgtcaac aactgcctaa catgttgatg atgaatgggt
     1981 acgataaaaa agcgcaaccc tctcagcagg gttgcgctgc aacttgaatg ttactaaatt
     2041 ggactctttt tttataatca gcacagatgt gctttcagaa tatcagcagt ttttttgttt
     2101 tttagttttt ttatggcttt gtccttgacc tgacgtaccc tttctctggt aatgtcaagc
     2161 ctgtctgcaa tttgttctac agaatattga tcatcgcact caataccaaa atacattttt
     2221 acaacctgac gttcacgggg tttcagttgt ttcaaagtta tcattaagtc gtgctttacc
     2281 gactcattgc tcaggttaaa atctacgggt tggttagagg ggtcactgta tagttcgtgc
     2341 atggaagagg attcatcgtc attactaacc ggtgaatcta atgaaaccgg aaacatattt
     2401 accgtcatgg cttcttttac tgcctgcggt gatgttttca tggcttcggc caactccagc
     2461 aaactcattt cacgaccatg ggtctgttca aattcactct gtaatttttt tattctcgaa
     2521 aactgtgaaa cctgattgaa aggaaggcga accaagcgca ctttctctac caaagcaatc
     2581 atgatagttt ggcgaatcca ccatacagca tacgatatga atttaaatcc tttggtagaa
     2641 tcaaaacgtt gtgctgcttt aatcaatccg atgttacctt cattaataag gtcttctaaa
     2701 gtcagcccct gattttgata ttgtttggca acactaacca caaaacgaag gttggattta
     2761 atgagtttgt tcatggcctg ctgatcgcct gtctttatgc gtcttgcaag ttggtcctct
     2821 tcgttgggag ttaataggtt ttcctgtgaa atttcgttca ggtaacgttt cagacattcg
     2881 ttttcgcgaa tggtaatcat gtttgtaatc ttcagctggc gcattgcgaa ttggttttta
     2941 aatgttatac ttgattgggt gattttattg taggcaacaa ccttgccaaa taattcatgc
     3001 aatccacatc tctgcttatt ttaagaaggt ggttttattc acaattggca acctgaatcg
     3061 gagcttgatt ttttattttc tcaatgattc aggagtgtag gtgagtattt aaaaacggat
     3121 tcagccaatt tggatattgg aaaaaaataa tggaattttt tatttcagaa aagttcaaaa
     3181 aaaaatgtag ttttgcacgc gttttataaa cggcattatt gaaaaataaa agctgtagat
     3241 gaaatttccc agcgcatgtg gcgtaattgg tagccgcacc agacttagga tctggcgggg
     3301 aaacccgtgg gggttcgagt ccctccatgc gcacgcaact gataaagctg aagctgtaca
     3361 gcttcagctt attttatgaa ataatatgta atatgaatat ccaaaaggaa gacgtagccc
     3421 cgttaaactc actcatcaga ataaaactga cacccgaaga ttataaaacg gatgtagaca
     3481 gtgctattaa aaaatttaaa aaatctgctt caatgccggg tttcagaccc ggacatgttc
     3541 ccgaaggcat gattaagaaa atgtatggca aatcttttct tgccgatgag ataaataaaa
     3601 ttgtaagtaa atcactttat cagttttttc gtgataataa tattgccgta ctgggcgaac
     3661 cacttcctaa agaagtagag aaccaaaaca attttgaaaa cccaagcgat tttgaatttt
     3721 tgtttgaaat aggtcaggct cctgttatta acctcgattt gccttcgctg cctgctgtac
     3781 attattatca gattgaagtt gatgaaaaac gcgttgatac ctatatagaa gatctgcaac
     3841 gcaagcatgg aaatttttca aatcccgaaa catctgaaga aagctccatt ctatatggtg
     3901 agtttattga gctgaatgaa aatgaagagg aaaaggaaaa tggcattcgc agtacaacca
     3961 cattggctat taatcttgtt aaagacgaaa cgtcaaaggc aaaacttatt ggtctgaaga
     4021 aagaggatga agtgacactt gacctgatga aagccatgca ggatgtaacg gaagttgctt
     4081 atatgttacg cgttgaaaaa gaaacagcac aacaaattac atctctgttt aaatacaaga
     4141 ttgcatcaat aaataaagtt gaaaaaatag aagtaggaca ggagcttttt gataaagttt
     4201 ttggagaaaa tgcagtcacc tcagttgatg atttccgcag taaagtaaaa gaagatattg
     4261 cagcaatgtt tgcacgcgag tcaatgcata aatttaacca tgatgttgaa gatgcactgc
     4321 tgcaccattt gaatatttca ttgcctgacg actttttgaa gcgctggata atggaagtta
     4381 acgaaaagcc tctcacacct gagcaaatag agcaggatta tcctgcatat tcgcgacaga
     4441 tgaaatggaa gatgatagaa ggtaagctga ttaacgacct tcagttgact gccgatgagc
     4501 atgaaatggc tgagtatgca actgctgtta ttatgcaaca gttcggacag tatcagctag
     4561 gcccggatat gattgcagat tttgcaaagc gctatcttca aacagaagaa aacagaaata
     4621 aagctgatga agcagtaaaa agcagaaaag tttttgatta tctgaaccaa aatgtcagca
     4681 aagacgttaa agttgtaagt tacgatgagt ttgttactat cgttaaagaa cataaacatt
     4741 aataaataat caaccggagt caatatgaat tttaaaaacg aattttttaa atatgccacc
     4801 aggcatcatg gcatcaacag tacggccgta ggtcattaca ttgaatctgc tgttcgtgca
     4861 gaaggatata tttcaccaac aatcattgaa gagcgtcaga tgaatgttgc cagcatggat
     4921 gttttttcaa gactgatgat ggacagaatc atttttttgg gagtgcctat taatgattat
     4981 gttgccaaca ttgtacaggc acagttgttg tttcttgaat caacagatgc taaaaaagat
     5041 attcagattt atatgaatag tcccggaggc tctgtttatg caggtttagg aatttacgat
     5101 acgatgcaat atattaatcc cgatgtggca actatttgta ccggaatggc agcttctatg
     5161 gcagccgtgt tgttgtgtgc cggtgccaaa ggtaaacgta ctgcgctcaa acatgcacgc
     5221 gtgatgattc atcagccaat gggtggtgca gaaggacagg catcagatat tgagataacc
     5281 gctcgcgaaa ttcagaaatt gaagaaagaa ctttatgaga ttatagcttc tcacagcggt
     5341 aaagattacg atacagtatg gaaagactct gatcgtgatt actggatgac agccggagaa
     5401 gcaaaagatt atgggatgat tgatgaggtg ttggtaagaa ataaggcaac aacagataag
     5461 taataaagaa agagtaaagc aagttgaaaa agcaaaacga agtaaagtgt tctttttgtg
     5521 gcagagataa aagtgctaca caggttctca ttgcaggact taacggtcac atctgtgatc
     5581 agtgtgtgac acaggctgta gaaataattt ctcaggaatc tgcagatcag cagaaatcta
     5641 aaatcagttc ttcatttacc ttgcttaagc ctgcagagat caagaagcac cttgatgatt
     5701 atgttattgg tcaggattat gcaaagaagg tgatgagtgt tgctgtttac aatcacttta
     5761 agagaatttc cggtgtaggc aaaaaagata atgatgtgga gattgaaaaa tcgaatatca
     5821 ttatggtggg agaaaccgga acaggaaaaa ctttgttggc acgcaccatt gcaaaaatat
     5881 taaaagtgcc tttttgtatt gccgatgcta cagtcttaac agaggcaggt tacgtaggtg
     5941 aagatgtaga aagcgtattg acacgattgc tgcaggcagc cgattataat gttgcagctg
     6001 cagagcatgg cattgtgtat attgatgaaa ttgataaaat atcacgcaag agcgacaacc
     6061 cttccattac ccgtgatgtg agtggtgaag gcgtacagca ggctttgctc aaaatgctgg
     6121 agaacaccat cattaatgtt ccaccacaag gcggccgcaa gcatcctgac cagaaaatga
     6181 ttccggttaa cacgcagaat attctgttta tctgcggagg tgcttttgat ggtattgaaa
     6241 agattattga acgcagactg caatcacagt ctatcggata tagtgcacat cgcaatcagg
     6301 atatatttga taaaggtaat ctttatcagt atgtttctcc tgccgatttc aaaaagtatg
     6361 ggcttattcc ggaactgatt ggccgacttc ctgcaatttc atatctgcaa ccattagaca
     6421 gggcagcatt aagaagtatt ctcactgagc ctaaaaatgc actgataaaa caatatcaga
     6481 aactgtttga gcccgaaggt attcaattaa aatttgatga caacgtgttg gattttattg
     6541 tggacaaagc catagagttt aagctgggtg cgcgtgggct acgctccatc tgcgaaacca
     6601 tcatgctgga tttgatgtat gaattgccgc aggtaaagaa caaaaaagaa cataccgtaa
     6661 cattggaata tgcccgagaa aaaatagaca aatcaaactt aggtaatctg aaagttgcct
     6721 gagtaatagt tgaaaacaga attttgtttg aatccggatt gaaaaagaat tcggattttt
     6781 tgttttaaag aaaaggcgat ttgctttatt tgaaaaaaac tcaggcttct gtcaattcaa
     6841 actcataaaa ctccataata ggattggaga ggagtttttt acaggcttca tcaacctttg
     6901 cttgtgcatc ttttttatct gtagcctcaa cttcgcaggt aatgtgcttg ccaattctca
     6961 cgttggtaat catgggtaag ttaatgttgt gcatactggc agaaacggtt tttccttgtg
     7021 ggtctaataa tgctttatgt ggcattactt tgatgtgagc taagaatttc attgcaatga
     7081 attatgtagt ggtactgttt tttattaccg acaaaagtac gtacaaaata acaatcagtg
     7141 gcaaggctcc gattttaaaa accaacaata aaatggcagc tgatgcaagg agcaggtaag
     7201 ggaaaaaatt ttctctgaaa ctaaaattct tgaattttaa agaaaacaag ggtatttcgg
     7261 caatgagcag ccatgacatg gttaaaataa aaataatata agccaccgga tgttgaaata
     7321 gcatgtaaac cgccatttcc atccaattgt tgttttgcgt agtactattg actaaataat
     7381 ttgaaatcaa ggtaggaaaa gctgcaatgg caagggcgtt ggccggtgta ggcaatccga
     7441 taaagctgtt ggtttggcgc gtatcattat tgaacttggc taaacgcact gctgaaaaaa
     7501 cagccagcaa cagaggaagc cagggtaaaa tcaactccgg aaacggcatt gctgcattgg
     7561 tatgctgctg cgggttgact acaataaaaa gttgcatcat cagtatagca ggtaataccc
     7621 caaaagtgac tacatcagcc agcgaatcga ggtctttgcc aatggcagag tatgctttga
     7681 ggctacgggc agcaaaacca tctaagaaat cgaatactgc tgccagcagt atcatccacc
     7741 ctgcaccggt aatatggccg gtaaaacaaa atacaatgcc cacacatcca cttagcaaat
     7801 tcagacaagt gatgaagttg ggaatatgtt gtttaagaga cattattttt gttgatgttg
     7861 atatgttgga aaattaccat ttgatgtgct tttatttaat tggattgctg taaatttggg
     7921 tgtaaatatt aaaaggattt ttatattcag tcatgcttga caaagaaaga aataaatttc
     7981 gtcatttgtc gttggaaata aatatccaaa tcgttaattt atgagaaaaa taaccctgtc
     8041 atggtttttt ataatagctt gttttgcatc tctggcacag acagctccaa aatacagtaa
     8101 tgagttttta aaccttggcg ttggtggcag ggcattgggc atgggcgggg cacaggtttc
     8161 gcaggtcaat gatgtaacag caggttattg gaatcctgcc gggttggtac gttcccgtgg
     8221 cgacattcag ttggcattta tgcataatga atattttgca ggtattgcca agtacgacta
     8281 cttagcgttg acagcaccta ttgacactat gcgacacatt ggattttctg tccttcgttt
     8341 tggtattgac gatattgcca acaccattga tctgaaagat aacgatggaa atattgatta
     8401 caatcgtgtt acaggatttt ctgctgccga ctatgcattt ttagtttctt atgccagtaa
     8461 aagcgcaatc aacgggttag attacggagc taattttaaa atcatacatc gcaaagtagg
     8521 cgacttcgca aaggcctggg gttttggcct cgatgcaggc attcaatatc aggcaggcaa
     8581 ctggcggaca ggtgcaatgt tgaaagatgt tacttcaacc tttaatgcat ggagctatac
     8641 gctgaacgat cgtaccaaag aagtttttat tcagacaggt aatgaaattc ctgaaaatgg
     8701 attggagcta acgctgccac gattaattct tggtgtttca cgtcagctaa atatcaggaa
     8761 gtttactatc acacctgcat tagatctgga gacaaccttc gatggtaaaa gaaacacctt
     8821 ggttaaaaca aattttgcaa gcatagaccc gagagttggt gttgaaggcg gctataataa
     8881 tcttgtttat gtgcgtgcag gcgtgagcaa ctttcaacag gtaactaata tagacggaaa
     8941 aaagacaact actgttcaac cgaatattgg tgttggaatt aaattgcgta atctggccat
     9001 tgactatgca ctaacaaata ttggaaacaa taattatctt tattcaaatg tattttcatt
     9061 gcgattgacg gtcaataaac tgaaaaagac atgagaaaac tttacgcaat aattatttta
     9121 tttcttcttc ctgttttaag tttcgctcaa ttttcgaaca gttggattaa ttttaacgga
     9181 gcatcacctt attcagcaca gcaatatttc aaaattaaag tctggaaaca gggcatttac
     9241 cgtcttgatt acaatttgct gcaacaggca ggttttcctg ttggaatgaa ccctaatcag
     9301 tttcagattt ttaataatgg tcatgagcag tatatttatg ttgaaggtgc tcaggataat
     9361 tcatttgatc catcggattt tattgagttt tatggaaagc caaataatgg ttcggcagat
     9421 caggaactgt atgataatcc ggctgaccaa ttgaatcccg actatagctt gttttgcgac
     9481 acagcagttt attttcttac tgcagctatt ttacccggca caccggtgaa aagaatgatt
     9541 atggagaccg atgtgaattt ttcactttac ctacctgaga attatattac aaaaactttc
     9601 aaaagaaatt atacatcaaa atatctgaaa ggcgattcgc agggagatat agacccttcg
     9661 tataatggcc ctgaaggatt ttcaggtaac acctttgcca ctgccacacc tgcagataat
     9721 ttttcactac ccaatgtttc tacatttgct actgcgccaa tcccaagtgc ggatgttaga
     9781 atggttaatg ccaactatga tggccatttt catacattta atgttgaatt caacggcaac
     9841 actattttta ctgacaacac catttttggc tacggacgac atgattataa ttttaatatt
     9901 cctattgcat ctttgccttt atcaaacagt gtggtgttta gtggcctagc aaattccggt
     9961 ggaacaaacc ttatgtctgt tacctatttt aaacttcagt atccacatgc aaattctttt
    10021 aacggtgagt tagagccttt tcagtttttt tctgtcagca acggtggcag caaagcaaga
    10081 gtagatttca cggactttct gaacagcgac aattctactc gtttcattta tgtgatttca
    10141 ggtgatacag tttctaaagt aacaacagta aggacaggaa atttgcttca ggctttaatt
    10201 cctgttaatg gtggagaaaa gaattgcttg cttacaccgg tatctgcaca ttattcttct
    10261 gtagctgatt tttatttgtc accggaaaac agcgaccccg atccgcagaa gtttgcacgt
    10321 tttataaact acagttacaa tcagccacag tacgattatt taattgtctc acataaaaaa
    10381 ttatggagcg aagcagagaa ttatcgcaac ttcagaacgc agacaggata taatccattg
    10441 ctcgttgata ttgatgaatt gtatgatcag tttgcttttg gaatttacaa acatcctttg
    10501 gcaataaaaa acttctgtcg ttttgctatc actgcattta ataataaacc tgattacctg
    10561 ttgttgttgg gtaaggcagt atatcctgaa gaggttagag taaatccaac agccgatcag
    10621 ctatgtttgg tccctactta tggcaacccg ccatcagact ttatgttcag caataaaatt
    10681 gttgacacca cctatgtgcc acgacttgcc actggaagaa ttgcagcaca gaatgttact
    10741 gatgttacta actaccttga aaaggcacag gcacatgagg ctgcaagaca aaactgtcct
    10801 gacgattggc taaaacatgt gttgcatttt attggaggaa ataatggttt tgagcaacaa
    10861 agtatcaaag cttttatgga tcagaatgaa gcagccatta gagatacttc aatgggatgt
    10921 acagttacaa actattataa aatatctact gacccgatag agtatattca ggcaatagaa
    10981 ttacaagcac gaatagatag tggtgtaagc attatgacat tttttggcca tgcagcaggg
    11041 tcaacttttg atattgcaac agatattcca cagaactggc acaacaccgg acgttatcct
    11101 gtagtgtttg ctcaatcatg taatgtgggt gatatttatt cgccttcaag attgctgaac
    11161 gaagattttg ttctgcttcc gcaaaaagga tcagtaggat ttttagcaaa gccccgactg
    11221 ggaaatattc aggagttggg agaatattct gtacaactgt atcgcaatgt ggcatacaga
    11281 gattatgata aacctatagg tatggcaata aaacatgcat tggattccat gatacaaact
    11341 tattatgcga ataatggaat ggtttataaa tctactgcat taggtatgtt gtggcatggt
    11401 gatcccggat tgcgattagc accttcattg caacccgact atgcagtttc gcagccatca
    11461 ataacatttc aacctacaga agttacttca gaacttaccg atttcaaaat gaatattgct
    11521 atcagcaatt ttggtaaagc cactaaagat tctgtttcag tacatgtagt gcgaaaattt
    11581 cctgacaaca gcacagtagt tattgataca ttgattcctt acatacccta taaagacacc
    11641 ttgcaactca caatgaatgt tgaagtaaat aaaggaacag gactgaatta ttttgatgtg
    11701 tttgttgatt atgctggcac tattaccgag tgtaatgaat ttaataacag agtactcaat
    11761 gtgcctttgc aaataatttc tactgatatc actcccgtgt atccatttaa atatgctatt
    11821 gtccctgacc cttcagtaat cttaaaagca acaacagaca atatttatgc agcacagaaa
    11881 acttatcgtt ttgagttaga tacaaccgat gcttttaact ctccattttt aagattcaaa
    11941 acgtttacac aaaccggagg catcgttaaa tggccattac catttacatg cgacagtgga
    12001 agagtttatt attggcgtgt aagtcttgac ccatcacttt atccaacaac atttaaatgg
    12061 aaggagagtt catttattta tattccgcag aaaacaggtt ggagtcaggc acactttttt
    12121 cagtttaaga atgacagtta caccaatatt gtttatgata aaccgcaacg taagtgggaa
    12181 tatgaaaata cacaggcaga gttgcgtgtt tctgcatggg atgtacccgg acaggggatt
    12241 acttctgata atgttcaact taacaatcag ctgattgcaa ccggtggttg taactatcgt
    12301 gctatttacg cagtggtact tgattctatt tctttacaaa actggaacac acttgattta
    12361 gcagggaaat ttggtaacat caatcaacca ccttattacg attcttgtca ggcaacagta
    12421 aatggtagtc agggcttttt tgcatttgcc acagactctg cttatagtgt tggtggcaat
    12481 accatatatc ggaatctcga cagccttgcg aattttatca atcgtatccc aacaggaaac
    12541 tatgttgtgt tatatagcgt gactgaacat tttatgagta agtcatggtc cggacagttt
    12601 cgctttgcct tatcacaaca ggggataaat agcgttgata ccattcctga taccaagcat
    12661 tttattttct ttttcaaaaa aggatttccc ggaacagcac aaacagttgt tgcagcttcg
    12721 gacagtacaa aaaatattac attcactacc atgctcggtg gaaattggta taaaggctat
    12781 atgatgtcgg agattatcgg ccctgctatt caatggaaca gtttgcattg ggctaacaat
    12841 agtgtggagg ccatcaatac agatacgctt gcactcgata ttgttggtgt agatacattg
    12901 aattcggaac atgttttgta cagtgctatc caaccttcac agatagattt taacttgaac
    12961 attaacccat ttatatatcc gcgaatttat ttgcgtgcat atatggaaga ttctgtttat
    13021 cgtacacctc cgcaactcaa tcgctggcaa atatattatc aggaagttcc tgaagctgtg
    13081 ataaatcctg tgggttcttc atttcaatcg agcaacgttt ctcaaggaga taccattcat
    13141 tggaaaatgc cggtagaaaa tgtgagtctt tctgatatga gtgatttgct cgtagattac
    13201 tatctttttg ataaagataa tgtaagacga aatattactt ctacacgctt tcatgcttta
    13261 ttacatggtg acacattgaa tgcttctgta cggttcagta ctgctgctta tccgggactc
    13321 aactcattat ggatggaggt taatccgaga aatgatcaac cggagcaata tcatttcaac
    13381 aattatgcaa gaatagattt taatgttaac cgtgatgtta ccaaccccat cctcgatgtt
    13441 acttttgatg gacaacatat tcttaatggc gacattgttt ctgcaaagcc gcaaatcctc
    13501 attaagctca aggatgaaaa caggtattta gctttgaatg atacatcaaa gtttaaggtg
    13561 tatgtaaaga atcctgatgg tgtagaaagt cagatttatt ttgagacaac agccaattca
    13621 tcattgtcag aaaatttatt gcgctggaca cccgcacaac ttcctgataa tacatttaag
    13681 attgagtata atcctgtttt caacgttgac ggcatttacg aacttcgtgc tcaggcaaga
    13741 gatgaaagtg gtaaccttag cggtagtaac gatttccgaa tttcttttga ggtaattaat
    13801 aaaagcagca ttaccaatgt tttcaattat cccaatccat ttacaacgcg aacacaattt
    13861 gtgtttacac ttactgggtc ggaagtgcct acatatttta aaattcagat ttataatatc
    13921 agtggtaaag tagtgaaaga aataacacaa cagcaattgg gtaacattca tattggacga
    13981 aacattacag actatgcctg ggatggaaaa gatgagtttg gtgatcagtt ggcaaaaggt
    14041 gtttatcttt accgggtaat aactcaactt gatggtgatg atattgagca tcggaatact
    14101 actgctgaca aatactttag aaggggatgg ggaaaaatgt atcttttgaa gtaaggcaaa
    14161 tctttaatgg cctatgaaag cccttgccgg ttatttttca ctatggttaa agaagaatac
    14221 gtttcttctt tgggctttaa ctgttattca cctgatattt tttatactgg ctttacgaaa
    14281 tggcaacatt gctttttctg ttgacagtac cgaatattta aatcaggcag aaaatattcg
    14341 agaacatggt acatggtatg caggaaattc atcagcacca cacgatgtct ttttggaaag
    14401 cagaaggcca cctttgtatg gcttctttat ttttttagtg catattgttt catcaaataa
    14461 tttcttttta ctcgcattgc agaatttgat gagtattgca gtgctcatcc ttacagcctt
    14521 tatgttttgg ttgctgaccg gaaaacggat tgccaaccaa tggttagtgg ttttaccctt
    14581 attgctattt ccgacacaat ttatttatgc caacatgata atggctgatg tcttgtttca
    14641 gtttttaatt attagtggat ttttgttttt tattcagtcg gtaagaaaac ccaattcgtt
    14701 tttgtacttc tgtattttta tgtcgttggc tattttaacc aagcctgttt tttatgcttt
    14761 ctgtctgata gttccaatta tagccttgtt gttttatact aaaggcattt ataagttgca
    14821 gaatgtctgg atatcactga ttccattatt gactgtagct tcaatatcaa caattaacta
    14881 ttccaaaaca ggttattttc attacagcag tgtaaatgaa aagttcatct ctgaatatgg
    14941 tgcctatctt gcagtggggg ataaaggaaa tgtaaatgca cagcaaaaga ttgactcatt
    15001 gcttagtgaa gccaaaaggc aacctgattt taagagctat tgtaagtata ttaataaaga
    15061 aagtttgtat ctgattaaag agaataaagc acgttttgtg atgatgcaat taaagggtat
    15121 ggctaacttt tttattgatc atggccggtt cgaccttatg gctttttttg tgcatcctga
    15181 ttatgttcag acgcaagggt ggaagcagaa atatgaggag aagggatggc aaggtgtgtt
    15241 tagctatcta aaaacattta atccattttt attactgttt ctgattttgg ccagtatctt
    15301 taacataatt atggtggtaa tattagtcag gtttttgttt tcacataaaa tagcaattct
    15361 gtttcgttta cttttattga tgtttgtgat ttatatggta gtgttcacag gagttgttgg
    15421 ctgcagtcgt tatcgaatgg ctatatttcc cataatctgg atggcattta tgtggtttct
    15481 tgccgaaagg aaatctgtac atcataaaac attagtttaa aaccatgacg atgatatttt
    15541 ttagacctga ttttttgggc ttactttcaa gcctataaat tgatatatat taatttcagc
    15601 gattgcttaa tttagccatc ggattagaaa atatttttga tgcagaaaat tcagttaaca
    15661 agcagctacg aggaatataa ttcattaaac gaattgccgg cagagtataa aagtttgatg
    15721 ttggaagcac atgatgcagc aaagacagct tatgcaccct actctgcttt tcatgtagga
    15781 gcagcagtgt tgttagcaaa tggaaaaatc ataaaaggct caaatcagga aaatgctgca
    15841 tatccttccg gtttgtgtgc agagagagtg gctgtttttg catcaagtgt tcagtatccc
    15901 ggagttaaaa ttaaagcaat tgcagtaaca gctatgccgg gaaataagga ggtgcctgtg
    15961 atggcaagtc cttgcggagc atgtcgtcag gtaatggtag agtatgaaca cctttatcat
    16021 caggatatta agtttatcat gtatggttca gtaaataaga ttattgtact cgacagtata
    16081 aaagccttat tacctttttc gtttaattca gatcagttat caggtaagta agcaaacagt
    16141 atttaactat ttttgtagca ttataaagaa tatggctaaa cagaaattta caaaggagac
    16201 ctatcttttt tggtatgagc aaatgttgct catgcgaagg ttcgaagaac gcgcaggaca
    16261 attgtatgga atgcaaaaga taaagggatt ttgtcatttg tatatcggac aggaagctgt
    16321 tgttgcagga gcaatgtcag ttatcaaacc tagcgacaat atgattactg catatcgtga
    16381 tcatgcccat gcattgggta aaggaacttc ggccagagca gtcatggctg agctgtttgc
    16441 taaggctacc ggatgcagta aaggcaaagg tggaagtatg cacttgtttg atgctgctaa
    16501 tcgttttttt ggcgggcatg gtattgtagg cggacagatt ccattaggag cagggattgc
    16561 atttgctgag caatataaag gtacagacag tgttacttta tgttatatgg gtgatggtgc
    16621 agttcgtcaa ggtgcctttc atgaagcatt gaatatggca atgacatgga aactaccggt
    16681 agtttttatt atcgagaata ataattatgc catgggcact tctgtagagc gaacaagtaa
    16741 tgttaccgac ttgtataaat taggtactgc atttaatatg cccagtgaac ctgttgatgg
    16801 tatgacggta gaaactgttc atgaagctat ggaaaaagca gtaaatcatt gcagaaaagg
    16861 gaacggaccg tatcttttag agatgaatac gtatcgttat aaaggacact ctatgagcga
    16921 tcctgcaaag tacagaagca aggaagagtt ggaaaaatac aaagcacaag atccaattga
    16981 agaagtcctg aaggtaattc agaaaaataa acttgcctca caagacgaga ttgatgcaat
    17041 tgataaaaaa gtacatgata ttgttgaaga cagtgtaaca tttgcagaag aatcaccatt
    17101 tcctgatgct gatgaactgt ataaagatgt gtatgagcaa tccgattatc cttacattat
    17161 tgaagattaa ttaacaagca ttacaagtaa aatcaaacat ggcagaaata gtaagaatgc
    17221 ccaaaatgag cgacaccatg acagaaggtg ttgttgctaa atggcataaa aaaattggtg
    17281 accagataaa gtcaggcgaa ttggttgcag agattgaaac cgataaggcc actatggagt
    17341 ttgaaagtta tcagaatggt acattacttt atataggtgt tgaagaagga aaggctgcac
    17401 ctgttgatag tatattagca atattaggta agcctggcga agattttaaa ccattgcttg
    17461 atgcagaatc aaaggcaaaa cctgtaacag ctgctgcacc tgaaaagact gaggcaaaac
    17521 ctgcaacgaa agttactgct gaagttcctg caaaatctgt tgctgcacca aaagcagaat
    17581 caatacctgt tgcgcaaaat gtagtagtgt cagcatctga cagcagagta aaagcatcac
    17641 cattagcacg ctcacttgca ggacagaagg gacttgattt atctatgata aaaggtagtg
    17701 gagataatgg caggattgta aaacgtgacg tggaaaggtt taaacaaggc aacgttgttg
    17761 caggaagtta tacttcggca atacacacac aggagtcgtt tgaagaaaca cctgtttctc
    17821 aaatgcgcaa aacaattgca cgaaggttaa gtgaaagtaa atttactgca ccacattttt
    17881 atcttacatt agatattgaa atgtcaagat gtatggatgc ccgcgaagct atcaatgctg
    17941 ctacaggaag taaagtttca ttcaatgatt ttgtgattaa agcatgtgct gcagcattac
    18001 aacagcaccc taaagtaaac tcatcctggt taggtgagac tatcagaaca aatcatcaca
    18061 tccatattgg tgttgcagta gcagttgatg aaggtttgct tgtacctgtt gtacgttttg
    18121 cagattcaaa aacattatca caaattaatg ctgaagtgaa gggatatgct cagaaggcaa
    18181 aggataagaa actacagcct gctgactggg aaggaaatac ttttacaatt tctaatctgg
    18241 gtatgtttga tattgaagag ttcacagcaa tcatcaatcc acctgatgcc tgcatacttg
    18301 cagtcggtgc aattaaagct gttcctgttg tgaaagatgg taatgttgtt gcaggaaatg
    18361 tgatgcgtgt tacgcttagc tgcgatcatc gtgttgttga tggtgttaca ggagctaagt
    18421 tcttgcagac tgtaaaagga ttccttgaaa accctgtatt gcttttaggg caagcgtcta
    18481 tctgattaac gtaacctgac cattgtaggt gtgatatatg ccaaactgat ctttgatttt
    18541 ggcagtgtaa acataaacac cttgcggaca aattctacct gaagtgtcaa tgccattcca
    18601 tttggcggca acagatggtg tttcaaaaac tttatttccc cagcgattaa atatctgcat
    18661 tgtcagttcg gtaatgccgt agccgtttgc aaagaaataa tcattgctac catcattgtt
    18721 tggtgtaaat gcattgggga tataaacggt aaatgcacct tcgacaattg cttccttttt
    18781 tgcagtgtca caacaaccaa attcactttc aacaatttga ataactctaa acctgccggt
    18841 atcctgatag ctatatgcag gattttgcaa cgtagaagtt tgcccatcac caaaatcata
    18901 aaaccagatg gagccaagca aactactatc agaaatattt atttcaggat gtaaaattga
    18961 aatactttca gggctaatgt tgaatgaggc aaccggcaac ggataaactt caattagatt
    19021 tggcataatc atgttgctta cacaattatc aggagttatt gcctgaatgc ttactgtgta
    19081 aatgccgtca gtgttgtata ggtgtgttgg tgtaatggag tcatttgttt gcccatcacc
    19141 aaaattccat gtaaatattg ttcccagagg ataggtggag ttgttgagga attgtacttc
    19201 taagggcata catccttttt cagtcagcgg tgaaatatca agcaatggtg tttcatttac
    19261 cacaaccggt atcattgctg ttgccggaat ttgagtacag ttgtccgtaa tactgacagt
    19321 atatgttgtg gtaaccgatg gcgatacatt tacaatggaa ctatcactac cgtttgacca
    19381 aagataaaaa tatgggccgc cattaccgcc attagcaata gatgacagaa cagcagactg
    19441 tcctgcgcaa attgtgtcat tgccagttgc agcaattcct aacggaggaa aaacatttac
    19501 taaaatacta tcagtttttg atgggcaatt gttgctgtct gtaacggaaa caaaatagtt
    19561 tgttgttact gatggtgtga ctaaaatggt tgatgcagca gtttgattat tccaaagcca
    19621 gttataggcg cctgttccac ctgttgctat tgcattgaga gttgcctgct gacctataca
    19681 gatggtatcc acaggattta ttgaaagtaa aatgggttgt ggttctgtaa tggtaaatgc
    19741 tttaactctg ctgcagttgt ttgcatcagt aactgtaaca acataaattc ctgcagtaag
    19801 atttgtgttt aatgattgtg taactccatt attccattga taactatacg gagcaacacc
    19861 tccctgaacc gttagtatga ctgatgcatc gttaaatcca ttacatgaaa cattgttgat
    19921 agttgaattg ggtttaatct gttgtggtga agtaatttgt tctgtaaaag aattatcaca
    19981 accattggca tcagtaatgg tcaccgtata gctgccaaca ggcaggtttt gtatggtgtc
    20041 atttgtttgt tgctgataac tccacaaata gctgtaaggt gcatttcctc ctaaagcagt
    20101 tgctgttagc attccattac ttccaccata acatgaaaca tcattttttg taaatagatt
    20161 tgcgataggc gcaggtgtag aaatttgaat ttgttcagtc agtgtgcagc cttttttatc
    20221 agtaatatcc acagtatata aacctgcggg tagcgcattg gcgtagttag tgtttcctgc
    20281 aattggtgac cagttgtatt gatagggtgg agtgccacct gttacagcca ccattgcaga
    20341 accatttgtg cttcctgaac atgttccgtt ttgagtagtg tgatttgctg ctaaagctgt
    20401 aggacttgat aaataaatgc tgtcagattt gttgcaatta tgtgcatcgg ttattgtaaa
    20461 cgaatactga cctgatgcaa tgcctgaaag accgaagcct gtacttcctg acgaccacaa
    20521 aatattatat ggaggtgttc cgccatttgc taccacattg agtgagccat tattgtatcc
    20581 gaaacaggta ggttgtactg aagtaatatt gctttgtact gatgtaggct gacttataga
    20641 aaaggaatga ctgcttttac aattgttagt gtcacgtaca taaacagtat agatacccgg
    20701 aagtaaagca ttaaagaaag tatctgcctg ttgtgtaata aagttatttg ttgagaaact
    20761 taaaacacca ttaccgcctg ttgttgtaat ttgaacttca ccatcttgca aaccatagca
    20821 agaaacatta gtcgttgtca gggaaataat ttgaggacct gttagattaa caatattaac
    20881 ctgatctaaa gtacggcagt ttttggaatc tttcactacg atgtaatagt tgctatttgc
    20941 taactgtgta aatcctgata cattaaaaaa tgttcgtcca ctatcagatg aaaataaata
    21001 aggtgaagtg cctcctgttg acgaaactgc aatacttcca ttaccggctg aacatgtttc
    21061 aggagtaata tttaataaca gttgtaatga gtcaggttgt aataaagtta taatggtgtc
    21121 gcttctgcaa ttgttctgat ctattaccga tgcagaatat gttccagcag ttaagttgct
    21181 aaacgtgttt gatatttgtg tggttattcc attgtcaata ctgtaagtta aagaacctgt
    21241 tccaccactt gccagaattt ttatttcacc atcattggtg ccaaaacatt taacttgctt
    21301 taaaacagaa ccaataagtt taggagcaag aatattactt agagaaactg cccgctgtac
    21361 ttcacaatta ttagcatctt taattttaat gctataactt ccggcataaa gattggaata
    21421 atttgtaatg ttgttataag aagatccgtt taaagaagta agtaacatac cggtaccacc
    21481 tgttaattgt aaattaatag aaccattgtt ttgtccacaa gcaggattag aaactgttgg
    21541 tgtgacctga attgctgatg gtgttgttaa ttgtacaagt cctgacacta aacatcctgc
    21601 actgtctgtc accgatactt gataggtgcc tgcagccagg tgattatagt tggccgtatt
    21661 tataaaagct tgattgttta agctgtaact caatacaccg gagccacctg atgccaggat
    21721 agaaattgtg ccgctgttta ttccaaaaca atttaaattt tgtgttatca ctgtatctaa
    21781 tgtagggccg ttaatggtat ttatttgtgc agaaaaatta ttgttgcaca tcaaggcatc
    21841 cttcacttgc aattgttgtg ttcctgcagg tagcgagcca aaatatgtct gttgactgaa
    21901 cggaccaccg tttagactga ataataaagt tccgtttcca ccttgtgcat taactgttat
    21961 actgccattg ctttgttggc agttagcatt ggtgatgttt aatgttgctg taatttcatc
    22021 aggcgatgtc agttgtatta tggtatcagc cgtacaaaaa ttctgatccg aaatttttat
    22081 atgataagaa ccggcaggaa ggttggtaaa gttcggcaat gcctgatagg ttacaccatt
    22141 gttaatacta taattcaata atcctgtacc accactagcc gataaagaaa taaaaccggt
    22201 gctgtcacca aaacatgtaa tatcactttt actaagattg gtaagtacgg gtgccgcctg
    22261 attaggaaca gaaattaatt tagttgcata acattggttg gcatcagtaa catgtagatg
    22321 ataaattcct gccgtaagat tattaaaaat ggtgccggga ctaaagttga tactatctaa
    22381 tgaaagttga agagttcctg tgcctccatt cacaacaatt tgaaaagaac cattgttctg
    22441 attgcaagta gctgctgtaa ctgcttgttg aattattatt gaatcaggtt catttaatgt
    22501 aatattagac gaagtaatac agccaacact atcagtaaga acgatgttgt agtttcctgc
    22561 tgcaagcatc gtaaagtgat tactcgaaac agatgattgt ccattgtcaa tactatacga
    22621 taatggaccg gttcctcctg aagcagcaat tgtaatttca cctgtttgct gaccgtggca
    22681 ttttatatca acagaagcga tattattaat cacagcacca ttaatagtat taattgaagc
    22741 tacaaatgca gtttgacaat tgttagcatc ctgaataatt atattttgat ttccggatga
    22801 caaattgttg aatattgttt gtgagctaaa cgcgccacca ttcagtgaat aagttaaact
    22861 acctgtgcca ccggaagcat taacagttaa agagccattg cttagtgtac aatttgatga
    22921 ggtagtttgt atgttggcaa taattgcatt gggttcggtc aataatatgg tggtgtcgga
    22981 ggtgcatcca ttagtatcac gaatagtaat tgtatagcct cctgctgata attgattaaa
    23041 tatgtttgag ttttggaaaa ttattccatc aagactatat tgtaacgttc cattaccggc
    23101 tgctgcaagt tgaattacac catcgtttga ctgatagcaa ctaagattgg aagtaatggc
    23161 ttgattgatt gttggcttat caagattatt tatctgaaag gcagtttgtg tagtgcagcc
    23221 attaaaatca gttgtgatga ttgaatagtt gcctgcacct aactgagtaa aaaaagtgtc
    23281 agttgcagtt gtgttattac ttagttcaaa ataataggca ggtgtgccac cgcctgcaag
    23341 aattgaaacc ataccatttt ggttactgca agtttcatta tttactaggg tttgtactgt
    23401 cagtaatggt ggttgtgtaa gtatgacagt atcttttacc tggcaatagt tagagtcttc
    23461 aactgtcaaa taatatgttc ctgcaattaa ttgattaaaa gttgaattag caaaaaaggt
    23521 agatccgtta tctatactat aggatagatt tccatttcca cctgatgcag ttatctgaat
    23581 tgctccatca ttacttccaa agcatattgg attggtaata gtattattgt caatctgagg
    23641 cgaatgtaaa atgggaatat gtaaggcaag agtatcagta caaaggttag catcacgaac
    23701 aatcaaagtg caacttccgc cattgagatt gtttatgttg tttgcaatgc tgtaagttaa
    23761 tccgctgtca gcactataaa ataaagtgcc tgttccactt atcgcattaa ttgtaactgt
    23821 agcatttgct gcattatcac aaatttgtgg tgtaaaacta tgcccggtta ttcttggtcc
    23881 cggttcgtca attatgctaa cggtgctggc ctcttcgcag ttgtttgtat cacgtattac
    23941 aattttataa cttcccggta taagattggt attattgtta ttagcagtaa aggtagcgcc
    24001 tccatcaacg ctataagtat aaggtgttgt tcctccattg cctgtgatta caattgaacc
    24061 atttggataa ctgcatatag tattggttac tgcagaagta aatgacaaag gaaaaggctg
    24121 tgataattgt aacattgtgt cagctctaca tcctttactg tcttttaatg ttaagtaata
    24181 atttcctgaa atcagatttt gaaattgatt tgttgcctga aaagttgctc cactgtctat
    24241 gctataagtg ataggaggaa taccatcaat agcgtttagt gttatacttc ctgtattggt
    24301 gttgaaacat tttaatgatt gaacagaaac attatcaaaa gaaggtgagg ttgaagctgc
    24361 aatctgataa ggtcttgttg atgtacatcc gtttgcatca cgtactgtaa aagtgatagt
    24421 tccgggaggt agttgcccat aatagttagt tgttgaccat gtcagaccac catcagttga
    24481 atattctaaa ggtaaaacac cattatcagg atgcaggttg gccgagccat tattaaaacc
    24541 gcagctagtc gcaactatat cgcctctgat gattataggc ggaggttctt ttaccgatgc
    24601 taattgatat attctgcaat tgttgttgtc tttaattaca actgtatatg ttttcgcggt
    24661 tagggaattg aaagagttag ttgcataaaa agttgatcca ttattgatgc tatatgataa
    24721 agtgccaaca ccacctgaac caaaaatatt tattgctcca ttttgtgaac gataacaagt
    24781 taaggattga gtttttaaac tatcaatatt aggtgcattt atggtattta caatggcagt
    24841 gtctttaatc atgcaaccat tattatcttc cactaataca taataaactc ctgggctaag
    24901 gttgctgaat aagtttcctg tctgatagtt tgttccattg tcaatgctat aaagtaatgt
    24961 tccagaagaa ctactcgtaa tcgttaattc aattgtccca tcattgaggt tacatcctga
    25021 aggagtaatc acacttacca tattaatctg tggtggttgt gttaaaataa ctgtggtgtc
    25081 tgatttacat ccgtttacat ctgttacagt taactgataa tttcctgcag gaacattttg
    25141 aatgatgttt gaactttgtg aaagtgttag accactcttt tttatttcat aaacataagg
    25201 gctgatacca tttgatgcca gtactgtaat ttctccgtca gcgccattgt aacatgtgat
    25261 gttttgccag gtaaagctgt ttattacagg cactgtactt gcatttatag tagctggaac
    25321 agatacagta cagttatgtg catctttaat tgtaatatta taatttcctg aacttaaatt
    25381 agaaaattgc ggacttcctt gaaaagaaac accattatta atactatagt tatatggtgc
    25441 tgtccctcct gatgcagtta catttatcga gccgttaact aatccgcatt ttgttgctga
    25501 aacagttaag gtggcagtaa gtggttgtgg ttgtgtcact gcaaagctta ctgtactttg
    25561 acaaccatta acatcttctg ttatgagttg ataagttcca ggattcaggt tgctgattaa
    25621 gtcaattcca tttacaggac cattttgctg aatgattaca ccggaagaat ttttccaaat
    25681 ataggtccat ggccccgaac cttgtgcagt tccaattgca gagccttgtg tgccaaaaca
    25741 aacaggctgc gtaattgcca ccaccggtgg aatgcagcaa gcaagggttg catataaatc
    25801 gtgaaccgga tcattagcac aggcagcaga tgtccaagag ccactttccc catcggtgta
    25861 ggtttcaata aatacattca ggttggtgcc aaagttgcac aatgattgtg gtaatgtatt
    25921 tatcttccag caaaaattga gattacaatt ttgtccaagg ttatcaccga aattgtttcc
    25981 cggattatta tcctgagtgt tgctgctgtt tcctgcagaa gtttcataaa aaaatcccgg
    26041 accatatggc ttgttgttgg ctgatgaaat aattggattt gaataccatg accagaatcc
    26101 attgttggag cacgatgccg gtggcaatgc aattattgtt gtggtgtccc atcccggacc
    26161 aaatttaggt acaatgccat gcagccagtt tgcagaagta ggattgaaat ctgtaacatt
    26221 caaacaaaat gttacttgtt gccctgaaag ataaaatcca tttgaaggag gaggagacac
    26281 cgtcaatcca ctttgcatca cacaaccact acagtcaaaa ttgttttgaa gtgataatga
    26341 aaaattacac tgatcattga tgctgccacc actgacctga agataatagg ttcctgctgc
    26401 aagaccacca aaagttgtat gcaaatatcc gccaccgcca atagcacaac cacgtggaac
    26461 taaatttccg catcctgttc cggaataaag tccaacctgt ggtgttatta aaccattgat
    26521 tgtaatatca agtacaggag ctgtaaggct gaatttgtac catacatcgg tggcaggatt
    26581 agacatactt ccgataggct tacaaccttg aagtgactgg tatggaattt ctgcagttgc
    26641 acaaaggttt gatgtatttg tcagactaac tgcgccaatg ccattggggc atgcagcagg
    26701 tagtggtaat aagccaagat tctgtgcacc tgaacatgca tcgtttacag gaggagcacc
    26761 accgttgcat gtagggcaac catttaccgg acaatttata cagttgatat atgctttcca
    26821 tccacctctg ttaacggtgt aatctgaaga aaaccgaatt gttaaacaac ctgatgttgc
    26881 tgttatcgtt ccgggggagt tggttcctgt aaaagttcct aaaagcggag atgaagtaga
    26941 gggtccatta tagataaaaa gaaaatcaaa atctttttca attgcgaatg actgaaaact
    27001 tagctgcaca catttgccgg gctgatcact gcaaatggta taaacatagt tttcattgtt
    27061 gctgtaattt gtcgaatcac caccgctgtc aaaaaaagtt ccgctgcaac cgctcattac
    27121 accgttggac ataaaaatat cctgtgccag cgtaacgttt gcaacaaaat acaatacgca
    27181 aaaagcgata attcttggca atttggaaat accttttaaa tgaagcgtaa agctaataaa
    27241 aaattgttaa taacttagac ctgtgggtac ttcaacagca ctcatgtaat aagtgacaat
    27301 atagagtatt tactacaatg tttcaggtgg atttttgctt acttcgataa agtatgtaac
    27361 acgctgatac tgttatgata acagaggcaa ttaccatcca aaattcaccc tctctgattc
    27421 ctattttttc ctcgttaata ttaattgcat ttttttgttc ataataactg agtagcactg
    27481 ctgaaccatt gttaataaag tgagccagta tgggtgccca gatagagcct gtccaaacca
    27541 atagatagcc taaaaataca cccaacataa acctgggtaa gaacccaaaa aactgcatgt
    27601 gaatagcgct gaaaataaat cctgttatta ttacagccca atgttggttt tttgttgctt
    27661 gttggatgag gttttgaagt acgccacgaa ataataattc ttcgccaaca gcaggcagca
    27721 atgcaatcat cgaaaggttt atgattaaat caaaatttgt atgcgttact aaaaaagatt
    27781 cagtcagttt ttctgcttct gtttccatgt tgcgcatcat tttttccatt gccgctaatg
    27841 aagccggtaa ttgaagatga ctattccagt caagtagtaa attaattaat ggtgatagac
    27901 atattgtaac aacaacgata acagcaattt gcaagaaatt aatttttttg aattgaaaaa
    27961 acagactaat attatctgaa aaaagccagc cacatagcca ggctggaacg agcatcagtc
    28021 ccagttgcga aaatatctga ataaacttca tggcattgat gtaagttgta tcac
//
LOCUS       DAOQNP010000033        27982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_549_length_27982_cov_8.154009, whole genome shotgun sequence.
ACCESSION   DAOQNP010000033 DAOQNP010000000
VERSION     DAOQNP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27982
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_549_length_27982_cov_8.154009"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    157..256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(638..955)
                     /locus_tag="PKY09_06195"
     CDS             complement(638..955)
                     /locus_tag="PKY09_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12483.1"
                     /translation="MRKLFLKAVLILSTIAFIISYYDDYKKQKQMEYEQSLPPKPAWD
                     TATSPTYQHLKEVERKLEERMQNNKRQQKSKQMADSLTEEQRQQLQWIEDQVESEIEN
                     KMD"
     gene            1140..1724
                     /locus_tag="PKY09_06200"
     CDS             1140..1724
                     /locus_tag="PKY09_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB12484.1"
                     /translation="MKKLFLSAIAVIMAFSLNAQTKWTADKSHSKIGFTVTHMLIAEV
                     EGHFKDFTASMTASKDNFEDSKVEFKAPVSSINTNDDKRDEHLRGSDFFDMANHPEIS
                     FTSVTFKPSGDKKFKVVGNLTMRGVTRSVTLEATHMGTVEDPWGNTKAGFKLTGKVNR
                     KDFGIAWNKTLTSGGAVVSDEVAMNCMIELIKQK"
     gene            complement(1797..3101)
                     /locus_tag="PKY09_06205"
     CDS             complement(1797..3101)
                     /locus_tag="PKY09_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12485.1"
                     /translation="MQDEKFDDLIRQKLQTEEESVSAGLWSRVEADLPVAKQDLFNKK
                     LFWLILLLLIPAGAGWYYFTSNQTEESSIEGNDNNKPEMISNIASEEKNTEDKKMLNN
                     AVPPSDNHAINNSIKNNSGQINYSTLTTNSNTVPKTLVTTNVIAATTDFSNQVEISEW
                     DLLNPIQASLPEAVISQALIADKPNSEIRKTKKGVHVFADVYYAPEFAQHTRDLGSGS
                     PAYLKLRRNTETNIYSHSQGIRAGVWLNSKFFFKTGIDMFEMNERLSYTFIPGMGNLP
                     VGMRIDSINGYYINPLTNDLVADTIVGSVNATGEVIISNHFSFVNVPLMIGWRYNKGL
                     WNIQSSAGIYMNIYSGYKGKIIHDSMQSLIDISSTSSPYKQNIGLGVSAEVIATYSIF
                     QNIDVLIGPKYNYHLSDISRKGAPIREKLSSIGVFSGIRLNF"
     gene            complement(3091..3636)
                     /locus_tag="PKY09_06210"
     CDS             complement(3091..3636)
                     /locus_tag="PKY09_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB12486.1"
                     /translation="MTDRELIEGCIRGERKCQKALFEKFAGKMKVVCMRYVNNHEDAE
                     DVLQEGFIKVFKYISKFGFEGSFEGWIRKIMVNTALTKITRYSYNEEVALVHSNLPVY
                     DYGDVIAEMSAKELKAHINALPDGYRLVFNMYVIEGFSHKEIADSLNISEATSRSQLL
                     KARLALQQKLKKNKIAYSHAG"
     gene            complement(3820..4461)
                     /locus_tag="PKY09_06215"
     CDS             complement(3820..4461)
                     /locus_tag="PKY09_06215"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNB12487.1"
                     /translation="MTDTFKHQGLRRQLITELKSKGINDAAIIAAMTKIPRHAFMDNA
                     FMESAYIDKAFPIGSGQTISQPYTVAFQTMLLGIQKGDKVLEIGTGSGYQTAILCELG
                     AKVFSIERHKPLSDKAKTLLEKLGYMPKIHFGDGFKGLPAFAPFDKIIITCGAPDIPQ
                     LLFEQLKTGGVMVVPVGEDVQVMKTVVKKPDQTMEVSDFNSFRFVPMLGNKVR"
     gene            complement(4549..5493)
                     /gene="trxB"
                     /locus_tag="PKY09_06220"
     CDS             complement(4549..5493)
                     /gene="trxB"
                     /locus_tag="PKY09_06220"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNB12488.1"
                     /translation="MSEKSEHVKCLIIGSGPAGYTAAIYAARADLKPVMYQGLQPGGQ
                     LTTTTEVENFPGYPQGTMGPQMMEEFRQQAERFGTDIRWGYASSVDFKGPVHKVIIDE
                     EKEITADTVIIATGASAKWLGLESEQRLNGFGVSACAVCDGYFFRNQHVAIVGAGDTA
                     AEEATYLAKLCTKVYMIVRRDEMRASKAMQHRVMNTPNIEVLFNTETKEVVGEKTVSA
                     VKVVNNLSGEERVLDVTGFFVAIGHQPNTAIFKGWLDMDKTGYIITKPGSTKTNIEGV
                     FACGDAQDKIYRQAVTAAGTGCMSALDAERYLADRGIH"
     gene            complement(5502..6224)
                     /locus_tag="PKY09_06225"
     CDS             complement(5502..6224)
                     /locus_tag="PKY09_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12489.1"
                     /translation="MKIFSAFLFSILIYSNINGQQLAIENKPALNEESKSVDSWVVHL
                     DQDMSYCISTYSEFMRKNFDYRVSRRSKNVHSVDKKMFSEISSLRIDQRAVFTQESKG
                     TAVAFQFSPGYDIHFGNTMYKEEFDRARALVVNYVHFHYKMFYDDQIKSLRSKIKGLE
                     KNISNNQSKTEKNNKAISLNAEDISNNAVGADKLREKNEKLEKENVSLAAEVDKFRTQ
                     ITQLETEIDNANQSLKLVEAFN"
     gene            complement(6226..7494)
                     /locus_tag="PKY09_06230"
     CDS             complement(6226..7494)
                     /locus_tag="PKY09_06230"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNB12490.1"
                     /translation="MRIDVLLGLQWGDEGKGKLIDVLAPDYDVIARFQGGPNAGHTLE
                     FDGIKHVLHTIPSGIFRENKLNIIGNGVVIDPLIFKKENDSLLKLGIDIKKNLWVSRR
                     AHLILPTHRLLDAASETMKGKTKIGSTLKGIGPTYMDKTGRNGLRVGDIERHDFKDAL
                     QALLNKHAELLSFYNHPKVSKDDLEMWLESISTLREMTLVDSEYEINSLINSGKRILA
                     EGAQGSLLDIDFGSYPFVTSSNTITAGACIGLGVPPAAIGKVFGVFKAYCTRVGSGPF
                     PSELNDATGEELRKKGNEFGSTTGRPRRCGWLDLPALKYTAMINGVTSLMMMKSDVLS
                     GFENLQVCNSYKVNGQPSMRMPFNVVNEIPEPEYISFKGWQRDISKTNSFESLPSELH
                     TYIQAIEKYTGIPLDVVSVGPDRTQTIIRK"
     gene            complement(7509..7850)
                     /locus_tag="PKY09_06235"
     CDS             complement(7509..7850)
                     /locus_tag="PKY09_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB12491.1"
                     /translation="MNFSFTTSEKGNFCIIKLSGNLIEKGQANELMDEVSTCIIRDMK
                     SFVLDLTDLRFMNSSGLTVLLNILTKSRKAGGDTIICNINSQLKSVFTITRLTEVFTI
                     TDNQEAAIAAI"
     gene            complement(7847..8329)
                     /locus_tag="PKY09_06240"
     CDS             complement(7847..8329)
                     /locus_tag="PKY09_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNB12492.1"
                     /translation="MAEAEFLKTEKEIFTEYMVSNGLRKTPERYAVLEEAYRTNGHFN
                     VDMLFELIQKRKLRVSRATVYNTIEHLLKCGLITKHQFGETKFIYEKSFAYKQHDHLI
                     CGDCEKVFEFCDPRLYQIQSTVEKYLDFKISQHALNFYGQCNKLKQTGACEHFKIKKQ
                     "
     gene            8628..11411
                     /locus_tag="PKY09_06245"
     CDS             8628..11411
                     /locus_tag="PKY09_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12493.1"
                     /translation="MTLKSFFRLFIATLFAGLVSADHVMALYYQSVTSGNWNATSTWQ
                     SSATPVGPWAPAGATPTSADQTVTIMAGTVVRITTGATIDEVVVSPGGTLIARTNVSL
                     VINNGAAADLIINGTFIDSLTGSANITFNPGATWQISAGATIVKCTGSSCNNWQSAYQ
                     GGIISIPATANWIFRKVGTENPSVSTTNGGSQAYYPNFIYENYTSTPFITGSFANSHF
                     NGSVSYPIIKGSMDIGGSGTNYVNFTSNDVNATIGVQVRGDVIIRPGNTYNNIGTGTE
                     IWGDVYMDGTWKYYNGNGRRLVFSGNANSTIWGSGLFQIHELTMAKILPASITLNRSL
                     MVDSLVNFNAGKIYSSATNPLIFNVGAKANTVSNNNSFVEGPVRKLGTTAFVFPVGMA
                     NDVQPAGLSASGAPADSGVFWTEDFNNGCTSLCDANSYTGFNGSWSVINSGTNGNRAN
                     MFYVSCAEANRPVNTCAGGCATGGDATLHVGTSPCATCLICPTGDCGAIYDTLTSGGF
                     DPTTDKRAVSPPINTVGKSNMSISFKYINVGDLRSPASSTDKVILEYSIDNGLTWTAL
                     LNIPRSSLSGGCALYKRWTAFGPFSLPAACNDLPNLMIGFRWINNNNSIGATVNTNGS
                     FAVDDVSISGKSAESFTTEYFHINPTVIYNNIVNPPLDHVSRMEYWYMTKESGDKRKI
                     TLYWDGNSGGVTSLPDLRVARFNSASWDDLGNTGTTGTPAAGTITSDSTKDYGPVTLA
                     SVIALPGNPLPVELLSFNGNRHNDEIKLNWLTSSELNNHYFTLKKSGNNLHYTELGKV
                     NGAGTTNVAQRYDYTDSRPLRGINYYKLFQTDFDGTTVEKATLAVRFDAYDGDVLVLP
                     DYNHEQVLLQFSEANQSPVSIMLMDASGRLILQTVCSESNTCRLLTSQLSRGVYVLRM
                     QTADGVVVKRFFY"
     gene            11416..11889
                     /locus_tag="PKY09_06250"
     CDS             11416..11889
                     /locus_tag="PKY09_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB12494.1"
                     /translation="MIVLSEFIHQELGQKIIIAHPESQEELNQYYQLRHEILRAPWNQ
                     PPGSEKVNDDNSAIHLCLMIENLMAGVCRLHYNNADEAQIRFMGVKAAYRNQKLGDYL
                     MVAAENISKSNHCKSIFLQARENAVPFYERNGYHIEARTFILFDTIQHYAMRKNI"
     gene            11950..12936
                     /locus_tag="PKY09_06255"
     CDS             11950..12936
                     /locus_tag="PKY09_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB12495.1"
                     /translation="MLSIGVIGAGHLGKIHLKLLKELTSYRIVGFYDNDVNTRDQVSE
                     TLGITAYDNAERLIEASDVVDIVTPTLSHFKYAMLALNAGKHIFIEKPITHTLQEAEE
                     MIALSEQKGLKIQIGHVERFNPAFIAAQSKIHQPMFIEAHRLAEFNPRGTDVPVVLDL
                     MIHDIDVILSIVKSEVKLINTSGVSIISDTPDIANVRIEFENGCVANLTASRISMKNM
                     RKARIFQGDSYIAVDFLKKKTEIISMRNTSENESPFAIIIDPGNGKPNKTIEFNNPVV
                     EESNAIKEELRTFALAVENNTTPIVNAMDGYKALKVANQIMDKLSQQKFTER"
     gene            12933..13793
                     /locus_tag="PKY09_06260"
     CDS             12933..13793
                     /locus_tag="PKY09_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12496.1"
                     /translation="MMRLKQLFTVIAFASLMQACNLINPAEPTPAYIQIDTINLTTNL
                     TTQGSASHKIVDAWVYVDNQALGAYEMPCKFPVLATGEHTIYIQGGILLNGINATHLQ
                     YPYYKFVDKTVTLKPGEVVKIDNLSTSYIDNLTFPLMEDFSGLFSFEKSGNSPVGMLG
                     EFDASQGMEGGYGVVRLDSGIYNFEVKSKEMILPNNGTNVFLEMNYKNSAPFLVILQA
                     TDPFGTVINNAIITLNTKTEWNKIYIELKPTMQLTSTSKRFRVLFGMQRTDASTNESF
                     YFDNVKVVHN"
     gene            13817..15232
                     /locus_tag="PKY09_06265"
     CDS             13817..15232
                     /locus_tag="PKY09_06265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNB12497.1"
                     /translation="MNKRSQVIKYIIADFLSALLAWTMFFIYRKTCIEPGESPFTKEF
                     LSDPKFYSGIFILPVCWVLFYAFLGTYNNIYRKSRLRDLAQTALASLIGVTIIFFILL
                     LDDVIVTYKNYYNTFLTLLIIHYSITVFFRLIITTITGNKIKKRKIGFNTLLVGSSQN
                     ALHIFEELETQKISQGNKFVGFIHVDEKNGEYLGGKLPHLGGIADIPEAINKHLVEEV
                     IIAIESSEHENIGRIINELEDTNAIIKIIPDMYDILSGSVKMNSIFGAPLIEIYPQLM
                     PAWQQYAKRLMDIVVSFIFLVLLTPTFIITALIVKLTSKGPVIFKQERIGLHGNPFNI
                     YKFRSMYLDAESGGPKLSSKDDSRITPFGKIMRKFRIDETPQFFNVLIGEMSLVGPRP
                     ERQYFIDQIMLHSPHYRHLHKVRPGITSWGQVKFGYAENVGQMVERLKYDIIYIENMS
                     LALDFKILFYTVKIIFQGRGK"
     gene            15297..16187
                     /locus_tag="PKY09_06270"
     CDS             15297..16187
                     /locus_tag="PKY09_06270"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNB12498.1"
                     /translation="MKQIAVIGSGTMGNGIAHVCAQYGNNVSLIDISEAPLEKAIKTI
                     TGNFDRMVTKGIVTAADKDAALQRITTYTNLSDGVKNADLIIEAASENESIKLSIFKE
                     LDSVCKADAILATNTSSISITKIASVTKRPDKVIGMHFMNPVPVMKLIEVIRGYKTSD
                     EVTKIIFDLSVKLNKIPVEVNDYPGFIANRILMPMINEAIYSLYEGVAGVHEIDEVMK
                     LGMAHPMGPLQLADFIGLDVCLAILRVLHDGFGNPKYAPCPLLVNMVTAGNLGKKSGV
                     GFYDYASGGKDLIVAATFQK"
     gene            16188..17360
                     /locus_tag="PKY09_06275"
     CDS             16188..17360
                     /locus_tag="PKY09_06275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNB12499.1"
                     /translation="MALPYPEIRLKKGKEKSVLNFHPWIFSGALEKMPAQLNNGDTVT
                     LLSHDGEILGTGLFHHSSIAVRLLAFSKVELNVTFWIQKLSNALQLRKNIHLFNNKET
                     TAYRLVHGEGDGLSGLIIDIYGDCAVIQCHIKGMFSHRYIIAEALNNVFNQSINTIYD
                     KSEDSFFENNESRFLKGEKQSEIIKENGHRFYVNWFEGQKTGFFIDQRENRRLLSNYC
                     DSKNVINLFAYSGGFSIYALKSGAALVHSVDSSSRAENWANQNVQLNDAVNHQFFCED
                     VFTFLKETKNNYQVWVVDPPAFAKRLDAVRNAMIGYRNLNTAVFRKASPGSIIFTFSC
                     SQAVDALTFRKVIFQSAKEAQRQIKVLHILSQPADHPVSVFHPEGEYLKGLVLYVE"
     gene            17404..18204
                     /gene="kdsA"
                     /locus_tag="PKY09_06280"
     CDS             17404..18204
                     /gene="kdsA"
                     /locus_tag="PKY09_06280"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNB12500.1"
                     /translation="MPQLKHTATGNFFLMAGPCIVESEQMCMDIAGTVKEITDRLQIP
                     YIFKASFRKANRSRLDSFTGIGDMPSLEILRKVRQRYQLPIVTDIHESHEAALAADYV
                     DVLQIPAFLCRQTELLVAAARTGKTVNVKKGQFLSPESMKFAIDKIRQSGNHQVMVTE
                     RGTMLGYGDLVVDMRGIPEMKKFDVPVVMDVTHSLQQPNQASGVTGGKPELISTIARA
                     AMAAGADGIFIETHPNPAKALSDGANMLQLDLLEKLLVSLLKVQQAVR"
     gene            18363..19034
                     /locus_tag="PKY09_06285"
     CDS             18363..19034
                     /locus_tag="PKY09_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNB12501.1"
                     /translation="MNSKLIIFIVISAIHLIAHLMAFQWLVFITKPLLMPILLWYFSE
                     GLTFGKLTLIQKRMITALTLAWIGDIINLYSHINHWIFTAGIIIFLAMQVMYIIICFY
                     LIRFKQIKQLYLIFCTLVFIAYGSVLLWLLLPHLDEMTIPVLIYASALTLTGIASALT
                     KDQLPRKVFYCLLTGTLLYIITDSLDAINNFRASFPYAAFWIALTYLSAQMMITIAVK
                     KIKTN"
     gene            complement(19056..19955)
                     /gene="nusB"
                     /locus_tag="PKY09_06290"
     CDS             complement(19056..19955)
                     /gene="nusB"
                     /locus_tag="PKY09_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNB12502.1"
                     /translation="MQVLYTYYQSEGYNVTKAEKELIEGTSRLYELYLTLLQLLIELA
                     EQEQLYRIDVASKFIVNKREFIRSFEKTTFISWLKNDEQFIDNCKKHKISWQADMEAV
                     GKCFYKLRQHPSYRDFTMTNTEVVEMDWLLKLYKEEVQQSESINGVIEEKNIWWAESL
                     ELAHVMVIKTIRQFYTTDQPAILPLFKDEADDRDFMIRLLKETIKNDKEWTSIIAERT
                     KNWEVDRIALSDMIMLKMAVTEVLKFDQIPLKVTLNEYIDISKDYSTPQSKVFINGVL
                     DKIVEELRKEDKVKKTGRGLIES"
     gene            complement(20037..21125)
                     /locus_tag="PKY09_06295"
     CDS             complement(20037..21125)
                     /locus_tag="PKY09_06295"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNB12503.1"
                     /translation="MIEVKEMKETIATANSVFAQVTDMKHEQVVFCYDHETGLKAIIA
                     IHNTNLGPALGGTRMWMYQNENDALTDVLRLSRGMTYKAAISGLNLGGGKAVIIGDSK
                     KDKTEALFRKFGQYVNSLSGRYITAEDVGTSTKDMEYISKETKHVTGLPESMGGSGDP
                     SPVTAYGVYMGMKASAKEVWGNDSLSGKKVCVQGAGHVGSNLVKHLTEEGAKVYVTDI
                     NKDALQQVATTYKAEVVAPDSIFSLDVDIYAPCALGATLNTENIGKLKCAIVSGAANN
                     QLADEVVHSKMLLDKGILWAPDFLINAGGLINVYSELKKFSRAEALKQTEHIYNVTLE
                     IFKKSKAEKIPTLVAAMQMAEKRIYGGK"
     gene            21235..22989
                     /locus_tag="PKY09_06300"
     CDS             21235..22989
                     /locus_tag="PKY09_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB12504.1"
                     /translation="MSDLLTLNRFFVKYRGRLALGLIFVLFANYFGLLPAQITRKAID
                     FISTGTHHNYSDSAKTLTLYALGIFGVVFLRGVLLFLMRQTIIVMSRHIEFDMKNEIY
                     DQYQKLDMHFYARANTGDLMNRISEDVSRVRMYTGPAIMYLVNITVMIIVVFIAMMNI
                     SPGLTLVVLFPLPLLVILIYYVQKIINTKSEKVQEALSMLSTNVQESFAGIRIVKSFS
                     KEKDFAALFHTQAKNYFNQTMQLARINALFIPAIMLLVGISSVTVIYYGGILVDEKQI
                     TYGNIAEFVIYLNMLMWPVGMLGWIITLIQRADASQRRINSFLHTESEIKYNNTGIVP
                     ENVKSIELKNVSFSFGKNSDHALENINLQLSTGKVIAIVGTTGSGKTTIANLVSRLYD
                     PTQGEVLVNDIPLKNMNLEWWRRQIGFITQDVLLFSDSVANNIAFGTKEYDTESLTAA
                     AKTAMVYDEIVHFKDGFSTMLGERGVNLSGGQKQRISIARAIMKQPQFLILDDCLSAL
                     DTGTEEQLLQNLLPYFQHRITLILGHRISSVKHADEIIVLHQGKILERGTHLSLLQQN
                     GFYAKMVTEQLKKQAVKS"
     gene            23064..23912
                     /gene="prmA"
                     /locus_tag="PKY09_06305"
     CDS             23064..23912
                     /gene="prmA"
                     /locus_tag="PKY09_06305"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNB12505.1"
                     /translation="MNAYIAIHCKVEPVQPFSEILIAQLAEIGFEMFEEKEDGFDGFI
                     RKENFNESLLETIEFLQPNEFCTAHWQLEEVESQNWNEEWEKNYPPIRVKNIYVRAPF
                     HPDASDNDLEIIIQPKMAFGTGHHATTAMMMELMLETNIADKNVLDMGCGSGILAILA
                     SKLGCTSATGIDNDPNCVLNSIENLENNKIRNVEVLQGDASSLKDLKFEIILANINRN
                     ILLDDIASYNNALSDGGQLLVSGFLTEDVDRIKQSFGKYGLKLSRMKQNQNWVAMHFF
                     KSHSGK"
     gene            23909..25171
                     /locus_tag="PKY09_06310"
     CDS             23909..25171
                     /locus_tag="PKY09_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB12506.1"
                     /translation="MTHHKETKNPLNIIVIVAALGYFVDIYDLILFGIVRTPSLNGLG
                     ITEPDLVTEYGLHLLSYQMIGMLTGGLLWGIMGDKKGRVSVLFGSIIMYSIANIANAF
                     VQEISDYTSSSIINTYTVLRFIAGVGLAGELGAGITLVNETMTKEHRGYGTMIVVSFG
                     VLGAVFANILGHFVTWQSSYIIGGVMGLVLLFLRIGVYESGMFKNLQVHSLERGNLMM
                     LFNNRKRFLTYLSSIAVGVPIWYMIGILVVFSPEIAKMNGITGVNGGDAIMYFYLATC
                     VGDFLSAYWSQQWKSRKKVLKLYLIITMVITPVYLYLMGNASVSIFYAVCGLLGFASG
                     YWAVFVSTASEQFGTNIRATVTTTVPNMVRGSLAILTLLLAYFRDSLGLNMIDAMFAV
                     GVITAAGAFLSLLGIKESYAKDLNYIET"
     gene            25168..25530
                     /locus_tag="PKY09_06315"
     CDS             25168..25530
                     /locus_tag="PKY09_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNB12507.1"
                     /translation="MKKKTLVMGASPNPARYSNKAIHKLLYYNHPVVAIGARPDKVEE
                     VVIETGQIAFKGIDTVTLYLNEERQHPLEDYILSLHPKRIIFNPGAENESLKIKAEKQ
                     GIECVEACTLVMLTIGDY"
     gene            complement(25524..25775)
                     /locus_tag="PKY09_06320"
     CDS             complement(25524..25775)
                     /locus_tag="PKY09_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12508.1"
                     /translation="MTQSTFGQDQARPIKTGGRPTEVREINNTPVIMIHKSNDAHDKG
                     NSNMQQRKSPRCIKKVGACTEGGDRKKELKEPVRINENQ"
     gene            complement(25864..27195)
                     /locus_tag="PKY09_06325"
     CDS             complement(25864..27195)
                     /locus_tag="PKY09_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB12509.1"
                     /translation="MKLQLKLAIYNALSKALVVLAFGALMPFIVEKVVYNHIDQRLKA
                     RTSKVLRIIEKGGLNDIARESDCTFESYNILKEEFILIEPLSGMKDVKPTVITNEEWE
                     IDGEKLNHRIIRQSFIYDNQIYSLQIGEGIDSLNKLKNTIGRVALWATILTIIASIFV
                     DVGYARLLLRPLNEIVERKLKGTKDPVHFNDVPVQTTTLEFQQLDISLTELMQRIKSA
                     FLTEKEFISNVSHELQTPISILKSRFENMLMDEKIDDDVADKLDDSIRKLNRMSKIIK
                     SLLMISKIDNEQFLKNETVNIKALLQEIVEDFQEHLSTKNIRLYMQHLDDFEFPNANR
                     TLLFTLFSNIITNAIKYNVENGQIDISASKQNDVFVVEIRDTGVGIVKENLSHIFDRF
                     KKFNKSTEPSFGLGLAIVKTISDFHRLTLSVSSEANKGTTFRVEFPVSSFT"
     gene            complement(27218..27895)
                     /locus_tag="PKY09_06330"
     CDS             complement(27218..27895)
                     /locus_tag="PKY09_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB12510.1"
                     /translation="MNVLIVEDERSLSHEMEIYLTKQGLHCDVAFTGAKALEMMWVNS
                     YDFVLLDIGLPDTNGFEILKEAKKNAIDSAFIVLTARSETDDKILGLDLGADDYLAKP
                     FSLPELHARMQAILRRKHGLKVQIIDINGFMMDYQNRTLTYEETPVVLTKKEFDLLHY
                     LALHKGRVLSRSQLTEHIWGDVMDDDYDSNYIDVHVKNLRKKLAAHAPIEWFETVRGI
                     GYRLNAQ"
BASE COUNT         8537 a         5366 c         5023 g         8956 t
ORIGIN      
        1 agaactaaga caaagataaa atttgaaagc ccgtccgacc ggtgtcatcc gggcgagcaa
       61 atcactttat ggcgaggcat gctcgcactc tttgcggaga taaaatggca aggtaaactc
      121 cgccacttta tttgaaagtt tacccaaaca gtataannnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnngggc gggcctgtcc gaacagtata actgggcgga caaatcacaa
      301 ctcagcgtaa ttaaaaatga taatcgttat aatgaaagaa cttcgacact aaaaaatggc
      361 aaggtaaact ccgccacttt atttgaaagt ttacccaaac agtataactg ggcgggcaca
      421 tcctaccggt gtcatccaag cgggcctgtc cgaacggtgc aatccgggcg ggcctgtccg
      481 aacagtataa ctgggcggac aaatcacaac tcagcgtaat aaaaaacgtt aatcgcttta
      541 ttgaaagaac tccaccattc taaaataacg aggtaaactc cgcaacttta tttgaaagtt
      601 tacttgaagg aaatacctgt aaataaaaaa taaatggtta gtccatcttg ttttctattt
      661 ctgattccac ctgatcttct atccattgca attgctggcg ttgctcctca gttaaactat
      721 cagccatctg cttggatttt tgctgacgtt tgttgttttg cattctttct tctaattttc
      781 tttctacctc cttgaggtgt tgataggtgg gtgatgttgc agtatcccat gccggttttg
      841 gtggaagtga ctgctcatat tccatctgct tttgtttttt ataatcatca taatagctga
      901 tgataaatgc aatggttgat aaaatgagca ctgcttttaa aaataatttt ctcatcaatt
      961 ttataaatta agactaaaat tatttcttat tctttcttta tctctgatta atacttaatt
     1021 aagtatgtat ggcaaaaata caactgaaat tttaattaat ttttacagag ttaatctagg
     1081 ttaattttta atgcaaagta ttggtctatc attgcaccgt aaattaaaaa aaataaaaga
     1141 tgaaaaaact atttctaagt gcaattgcag taattatggc ttttagctta aatgcgcaaa
     1201 ccaaatggac tgcagacaag agtcattcta aaatcggctt taccgttaca cacatgctca
     1261 ttgctgaagt tgaaggtcat tttaaagatt tcacagcttc aatgacagct tcaaaagaca
     1321 attttgaaga tagtaaagtt gagtttaaag ctcctgtttc ttctataaac accaatgacg
     1381 ataaacgcga tgaacatctt cgtggatcag atttcttcga catggccaat catcctgaaa
     1441 tttcttttac cagcgtaacc tttaaacctt caggagataa aaaatttaaa gttgttggca
     1501 accttaccat gcgtggagtt accagaagtg taacacttga agctacacac atgggtactg
     1561 ttgaagatcc atggggaaat actaaagctg gatttaagtt aaccggaaaa gtaaaccgca
     1621 aagatttcgg tattgcctgg aataaaacat taacatcagg tggtgcagtg gtgagtgacg
     1681 aagtggctat gaattgtatg atagaactga ttaaacaaaa ataagtgtga attaatattt
     1741 ttcgaggcag tcttttttaa ggctgccttt ttttattcac tcaaaacgaa aattaatcag
     1801 aaatttaatc tgataccaga aaacacacct atgctactca gcttttcgcg aatgggtgcg
     1861 cccttccggc tgatgtcact taaatgataa ttatatttag gacctatgag tacatcaata
     1921 ttttggaaaa tactatatgt agctatcact tcggccgaaa cacctaaacc gatgttttgt
     1981 ttataaggtg atgaagtaga tgaaatatct atcagcgact gcatgctatc atgaattatt
     2041 ttccctttat agcctgagta aatattcata taaatacctg cgctgctttg aatgttccat
     2101 agacctttgt tatatcgcca tccaatcatt aagggtacat taacaaaact aaagtgattt
     2161 gaaataataa cttcgcctgt tgcattaaca gagccaacaa tggtgtcagc taccaaatcg
     2221 tttgtgagtg gattgatata ataaccattg atagaatcaa tccgcattcc taccggaaga
     2281 tttcccatac ccggtataaa tgtatagctg agtcgttcat tcatttcaaa catgtctatt
     2341 cccgtcttaa aaaagaattt gctgtttaac catactcctg ctctgattcc ctgactatga
     2401 gaataaatgt ttgtctctgt gttacgcctc agtttcaggt aggcagggct accacttcct
     2461 aaatcgcggg tatgttgggc aaactccggt gcataataaa catctgcaaa tacgtgaaca
     2521 cctttcttgg tttttctgat ttctgaattc ggtttgtctg caataagtgc ctgactaatt
     2581 acagcttccg gtaatgatgc ttgtatcgga ttaagtaaat cccattcact aatttcaacc
     2641 tgattactga aatctgttgt agctgcaatt acattggttg ttactaaagt ttttgggaca
     2701 gtgttactat ttgttgtgag cgtgctataa ttaatttgac ctgaattgtt ttttattgaa
     2761 ttgtttatag catgattgtc tgatggagga acagcattgt ttagcatctt tttgtcttct
     2821 gtattctttt cttcagaggc tatgttacta atcatctccg gcttgttatt atcatttcct
     2881 tctatagaag attcttcggt ttgattagaa gtaaaataat accatcctgc acctgcagga
     2941 attaaaagca atagtatcaa ccaaaacaat tttttattaa aaaggtcttg cttggcaaca
     3001 ggtaaatctg cttctaccct actccacagt ccggcactta ccgactcttc ttcggtttgc
     3061 aacttttgcc tgatcagatc atcaaatttt tcatcctgca tgactgtatg ctattttatt
     3121 tttttttaat ttttgctgta aggccaaacg tgctttcaat aactgactgc gtgatgttgc
     3181 ttctgaaatg ttaaggctat cagcaatttc tttatgcgaa aaaccttcta tcacatacat
     3241 gttaaatact aacctgtagc catccggcaa tgcatttata tgtgctttca gctctttggc
     3301 agacatttct gcaattacat caccatagtc ataaacaggc aaattgctat gaactaatgc
     3361 cacttcttcg ttataactgt agcgtgtaat tttagtaagt gctgtattaa ccattatttt
     3421 tcttatccaa ccttcaaacg atccttcaaa accaaacttc gatatatact taaacacttt
     3481 tataaatcct tcctgcaata catcttctgc gtcctcatgg ttattgacat agcgcataca
     3541 aacgactttc attttgccgg caaacttttc gaacaaagct ttctggcatt ttctttcacc
     3601 gcgaatacaa ccttcaatta gttcacggtc tgtcataagt gtattgaatg cattccattt
     3661 gtaaagactc gctacatcag acaaatgttg cctgactttt gcactttggt aaaattaatt
     3721 aataaatagt ataatattac ctcagcttgc caaaaagata agtgggagaa aaagacagcc
     3781 caaaaaaata tctatgctgt ttattaagac agaatgtgct taccgtactt tattgccaag
     3841 cataggaaca aagcgaaatg aattgaagtc tgagacttcc atggtttgat caggtttttt
     3901 aacaacggtt ttcataactt gcacatcttc accaacggga acaaccataa cgcctcctgt
     3961 cttcaattgc tcaaacaata gctgtggaat atcgggtgca ccacaggtaa taattatctt
     4021 gtcaaatggc gcaaatgccg gaagcccttt aaatccatcg ccaaaatgta ttttaggcat
     4081 gtagcctaat ttttcaagta atgtttttgc tttatcagaa agcggtttat gcctttcaat
     4141 agaaaacact ttagcaccta actcgcagag tattgccgtc tgatagccgg agcctgttcc
     4201 gatttccaac actttatcac ctttttgaat gcccagtaac atagtctgaa aagcaactgt
     4261 atagggttgt gaaatagttt gacccgaacc gattggaaag gctttgtcaa tataggctga
     4321 ttccataaat gcattatcca taaatgcatg acgtggaatt tttgtcatgg cagcaataat
     4381 ggctgcatca tttattcctt ttgactttaa ttctgtaatt agctggcgtc ttaatccctg
     4441 atgtttaaaa gtgtctgtaa tattcattgc aaacaaattt accactccca gaacaaggaa
     4501 ttattctgat gcaataaaat ttttaacaaa aaaatgctga tgattttttt aatgaatgcc
     4561 tctatctgcc agataacgct cagcatctaa tgcagacata caacctgttc ctgcagcagt
     4621 aacagcctgt ctgtaaattt tatcctgtgc atctccgcag gcaaaaaccc cttcaatatt
     4681 tgtttttgtt gaccccggtt tggttatgat atagccggtt ttatccatat caagccaacc
     4741 tttaaaaata gctgtattag gttgatgccc aattgccaca aaaaagccgg taacatcaag
     4801 cacacgttct tcaccgctta aattattaac aactttaaca gcagacacgg ttttctcgcc
     4861 tactacctcc ttggtttctg tattaaataa gacttcaata ttgggtgtat tcattactct
     4921 gtgttgcatt gcttttgatg cacgcatttc atctcttcgc acaatcatat aaacttttgt
     4981 acacaacttg gccaaataag tagcctcctc agcagctgta tctcctgccc ctacaattgc
     5041 cacatgctga ttacggaaaa aatatccgtc acaaacagca catgcagaaa caccaaaacc
     5101 attaagtcgt tgttcgcttt ctaatccaag ccactttgct gatgcacctg ttgcaatgat
     5161 tactgtatcg gctgtaattt ctttttcttc gtcaatgatt actttatgta ccggtccttt
     5221 aaaatcaact gatgatgcat atccccatct gatgtcggtg ccgaaccgtt cagcttgctg
     5281 acgaaattct tccatcattt gcgggcccat agttccttgc ggatatcccg gaaaattttc
     5341 tacttcagtg gttgttgtca actggcctcc gggttgtaat ccctgataca ttacaggttt
     5401 taaatctgct cttgctgcat agattgctgc tgtatatcct gcaggacctg acccaataat
     5461 caggcatttt acgtgttcac tcttttcact cattgcatat tttaattaaa cgcctcaacc
     5521 aatttaaggc tttggtttgc attgtctatt tctgtttcta attgtgttat ctgtgttctg
     5581 aatttatcta cctctgctgc caatgaaaca ttttctttct ctaatttctc attcttctcg
     5641 cgcaacttat ctgctcctac tgcattattt gaaatgtctt ctgcattcag gctaattgct
     5701 ttgttgtttt tttcagtttt tgactgattg ttgctgatat ttttttccaa gcctttaatt
     5761 ttcgatctca ggctttttat ctgatcatca taaaacattt tgtaatgaaa atgcacatag
     5821 tttacaacca atgctcttgc acggtcaaac tcttccttat acatggtgtt gccaaaatgt
     5881 atatcataac ccggactgaa ttgaaatgct acggcagtac cttttgactc ttgtgtaaat
     5941 acagcacgct ggtcaatacg aagacttgaa atttcagaaa acattttttt atccacagaa
     6001 tgaacatttt tacttctgcg gctaacacga taatcgaagt tctttcgcat aaattcacta
     6061 taagtagaaa tgcaatagct catatcctga tctaagtgta ctacccatga atcaacactc
     6121 tttgactctt cgtttaaagc aggcttattt tcaatagcaa gttgttgtcc gtttatattg
     6181 gagtaaataa gtatgctgaa taagaatgct gaaaatattt tcattttact ttctgattat
     6241 tgtttgcgtc ctatcggggc ctactgacac tacatctaat ggaatacctg tgtatttttc
     6301 aattgcctga atatatgtgt gcaattcaga tggtaaactt tcaaaactgt ttgtcttgct
     6361 gatatcacgc tgccaacctt taaaagaaat gtattcaggt tccggaattt cattcactac
     6421 gttaaacggc attcgcatag aaggctgtcc gttcacttta taagaattac aaacctgcaa
     6481 gttttcaaag ccgctaagca catcagattt catcatcatc aggcttgtaa caccatttat
     6541 cattgcagta tatttcaaag caggcaaatc gagccatcca caacgccttg gccgtccggt
     6601 ggtgctgcca aattcattac cttttttacg aagttcttct cctgttgcat catttagctc
     6661 agatggaaaa gggccactgc ctacacgtgt gcaatatgcc ttgaacacac caaatacttt
     6721 acctattgcc gcaggtggta caccaagacc aatacatgcg cctgctgtta ttgtattgct
     6781 ggaagtaaca aatggatagg agccaaaatc tatatcgagc agagagcctt gtgcaccctc
     6841 tgccaaaatc cgctttccgg aattaatcag actgtttatt tcatattcac tatccaccaa
     6901 tgtcatttcg cgaagtgttg aaatactttc gagccacatt tcaaggtcat ctttgctgac
     6961 tttaggatgg ttgtagaatg aaagcagttc ggcatgtttg tttaacaatg cctgtaatgc
     7021 atctttgaaa tcatgacgct caatgtcgcc aacacgcagt ccgttacgtc cggttttatc
     7081 catgtaggta ggaccaattc cctttagtgt tgaaccaatt ttagttttgc ctttcatggt
     7141 ttctgatgcc gcatcaagca aacgatgtgt aggtaatatg agatgtgctc tgcgagatac
     7201 ccaaagattt ttctttatat ctattccgag ctttagaagt gaatcatttt cctttttaaa
     7261 aatcagtgga tctattacca caccattacc aatgatgttt agtttattct cacgaaaaat
     7321 tcctgatgga atggtatgca acacatgttt aataccatca aactccaggg tatgtcctgc
     7381 atttggaccg ccctgaaaac gtgcaatcac gtcatagtcg ggagctaaaa catctatcag
     7441 ttttcctttg ccctcatcgc cccactgaag tccaagtaat acgtctattc tcataataat
     7501 tctattattc agatggcagc aatggctgct tcctgattat cggttatggt aaaaacttct
     7561 gtcaatcttg taattgtaaa gacagatttt agctgactgt tgatattgca aataatggtg
     7621 tcgccacctg ccttgcgtga tttggtaaga atgtttaata gtacggtaag tccgctgctg
     7681 ttcataaaac gcaaatctgt taaatcgagc acaaacgatt tcatatctct gataatacag
     7741 gtactaactt cgtccatcaa ctcattagcc tgtcccttct ctataagatt acccgaaagt
     7801 ttaataatgc agaaattccc tttttctgaa gtggtaaagc taaaattcat tgttttttta
     7861 ttttaaaatg ttcgcatgcc cctgtttgtt tgagtttatt acactgtccg taaaaattca
     7921 aggcatgttg tgatattttg aaatctaaat atttttctac tgttgactga atctgataaa
     7981 gtctcggatc gcaaaattca aatacttttt cgcaatcacc acaaatcagg tggtcatgtt
     8041 gtttataggc aaacgacttt tcgtaaataa atttggtctc gccaaactga tgtttggtta
     8101 ttaaaccaca tttcaataaa tgttcaatag tattataaac tgtagcacgg cttactctta
     8161 acttgcgttt ttgaatcagt tcaaaaagca tatccacatt aaaatgacca tttgtgcgat
     8221 aggcttcttc aagtactgca taacgttccg gtgttttgcg tagcccattg cttaccatat
     8281 attccgtaaa aatttctttc tctgtcttta aaaattccgc ttctgccacg ttaaacaaat
     8341 cctattttag ttgccacaaa agtaataaat caaactgtta taggatttcc aatgagagtc
     8401 aagattttat gagatgtatt ttgcatgctc tctattctgt aatgacgata aattactttc
     8461 tctacatctc tgatttttaa atgaaaaaat gaggtttgat accaacagcc aacaataaca
     8521 aaattcattt tctttcgtaa aaatttattt actgacaaca gtagaaaaaa tgcattgttt
     8581 tagaattata tcaagtactt ttgccaattg aaccatacaa cactgttttg acattgaaat
     8641 cgtttttcag gctatttatc gccacacttt ttgcaggttt agtatcagca gaccatgtta
     8701 tggcactgta ttaccagtca gttacatccg gtaattggaa tgccacaagc acctggcaaa
     8761 gtagcgcaac tcctgtagga ccttgggctc ctgccggtgc aacaccaact tcagcagatc
     8821 agacagtaac cattatggcc ggaactgttg tacgcattac cacaggtgcg acaattgatg
     8881 aagtcgtagt tagcccaggc ggaactttaa tagcaagaac caatgttagt ctcgttataa
     8941 ataatggtgc tgctgcagat ttaataatca acggaacttt tatagattca ttaaccggat
     9001 cagcaaatat tacctttaac ccaggagcta cgtggcaaat aagtgcaggt gcaacaattg
     9061 ttaaatgtac aggctcctca tgtaataact ggcaatcagc ttatcagggt ggaataatct
     9121 ctataccggc aacagccaat tggattttca gaaaggttgg cactgaaaat ccatctgtaa
     9181 gtacaactaa tggtggttcg caggcatatt atccaaattt tatttacgaa aattatacat
     9241 ccacgccatt tattaccggt tcctttgcta attctcattt taatggttct gtaagctatc
     9301 ctattatcaa aggcagtatg gatataggtg gatctggaac aaattatgtc aactttacaa
     9361 gtaatgatgt caatgcaact ataggtgttc aggttagagg cgatgttatt atccgacccg
     9421 gaaataccta caacaatatt ggaacaggta ctgaaatttg gggtgatgtt tatatggatg
     9481 gcacctggaa atattataat ggcaatgggc gcagattggt tttttccggc aatgccaaca
     9541 gcacaatttg gggtagcgga ctttttcaga ttcatgaact gacaatggct aaaattttac
     9601 ctgcaagtat tacattaaac cgttctttaa tggttgattc attggtaaac tttaatgcag
     9661 gcaaaattta ttcaagtgca accaacccat taatttttaa tgtgggtgca aaagcaaata
     9721 ctgtatcaaa caacaatagt tttgtagaag gacctgtaag aaagctagga acaactgcat
     9781 ttgtttttcc tgtaggtatg gcaaatgatg ttcaacctgc aggattatca gcctcaggcg
     9841 caccggcaga ttccggtgta ttttggacag aagattttaa taatggatgt acttcacttt
     9901 gtgatgccaa cagctacacc ggatttaatg gttcatggtc ggttatcaac tccggaacta
     9961 atggcaacag agctaacatg ttttatgtta gttgtgcaga agccaacaga cctgtaaaca
    10021 catgtgccgg aggttgtgct accggtggtg atgcaacatt gcatgttgga acttcgcctt
    10081 gtgcaacctg cctgatttgt ccgacaggag attgcggtgc catttatgat actttaacaa
    10141 gcggtggctt cgacccgaca acagacaaaa gagctgtttc accaccaata aacactgtgg
    10201 gaaagtcaaa tatgtcaatt agttttaaat atataaatgt tggtgattta agaagtcctg
    10261 catccagtac agataaagtc atacttgaat acagtataga taatggattg acttggacag
    10321 cattactaaa catccctcgt tctagtttat ctggaggctg cgcgctttat aagcgatgga
    10381 cagcctttgg tccttttagt cttcctgcag catgtaatga cttaccaaac ttaatgattg
    10441 gttttcgatg gataaataat aacaacagca ttggtgcaac tgtaaacacc aatggttcat
    10501 ttgccgttga cgatgtgagt atttccggta aaagtgccga atcgtttaca acagaatatt
    10561 tccacataaa cccaaccgtt atctataaca atattgtgaa tccaccactt gaccatgtaa
    10621 gcaggatgga atattggtat atgactaaag agagtggtga taaaagaaaa attacacttt
    10681 actgggatgg taacagtggt ggcgttacca gcctgcccga ccttagagta gcacgtttta
    10741 atagcgcatc atgggatgat ttaggaaata caggtaccac aggcacacct gctgcaggta
    10801 cgataacttc cgattcaaca aaagattatg gaccagttac tttagcatct gtaatagcat
    10861 taccgggcaa tcctttacct gttgaattgt tatcttttaa cggcaatcgc cataatgatg
    10921 aaataaaact caactggctg acttcttcag aattaaataa ccattatttc accttgaaaa
    10981 aatcaggaaa taatttgcat tatacagaac tcggtaaagt aaatggtgca ggcacaacca
    11041 atgttgctca acgctacgat tatacagaca gcagaccttt gcgtggtata aattattaca
    11101 aactttttca aacagatttt gatggcacca cagttgaaaa agctactctg gccgtacgtt
    11161 ttgatgccta tgatggtgat gttttagttt taccggatta taatcatgaa caggtattgc
    11221 ttcaattttc agaagcaaat caatcacctg ttagcataat gctaatggat gcttccggac
    11281 gactcatact gcaaaccgtt tgctcagaaa gcaatacttg ccgtttacta acgtctcaac
    11341 tttcaagagg tgtttatgtg ttgcgcatgc aaacagcaga tggcgttgtt gtaaaaagat
    11401 ttttctacta aagcagtgat agttctttct gaatttattc atcaggaact tggacaaaaa
    11461 ataattattg ctcatcctga aagccaagaa gaactgaatc agtactatca actcaggcat
    11521 gaaattctaa gagcaccatg gaaccaacct cccggcagtg aaaaggtcaa tgacgacaat
    11581 tccgccattc atctttgcct gatgattgaa aatttaatgg caggcgtatg ccgattgcat
    11641 tataacaatg ctgacgaagc acagattcga tttatgggcg taaaagcagc atatagaaat
    11701 caaaaattag gcgattattt aatggttgct gccgaaaata tctctaaatc aaatcattgc
    11761 aaatcaatat ttctgcaagc ccgggaaaat gccgtaccct tttacgaacg caatggctat
    11821 catatcgaag ccaggacatt catattgttc gataccattc aacattatgc aatgagaaaa
    11881 aacatctaat tgctcctgtt aattcactta aggtagctat tttacccgta ttttcgcgca
    11941 aattttttca tgctttcaat aggagttata ggcgcaggcc atcttggtaa aatacatctt
    12001 aaacttttaa aagagttaac atcatatcgc atagtaggct tttatgataa tgatgtaaac
    12061 acacgtgatc aggtttccga aactttagga ataactgctt acgataatgc agagcgtttg
    12121 attgaagcga gtgatgttgt ggatattgta acacctacac tatcacattt caaatatgct
    12181 atgttagctt taaatgcagg aaagcatatt tttattgaaa aaccaattac tcatacactt
    12241 caggaagctg aagagatgat tgccttgtca gaacaaaaag gtttgaaaat tcagattgga
    12301 catgtggagc gatttaatcc tgcatttata gctgcacaaa gtaaaattca tcaaccaatg
    12361 tttattgaag cgcaccgttt ggctgaattt aacccacgcg gcactgatgt acctgttgtg
    12421 cttgatctga tgattcatga cattgatgtt attttgagta tcgtgaagtc tgaagtaaaa
    12481 ttgataaaca ccagtggtgt ctccatcatc agcgatactc ccgatattgc caatgtcaga
    12541 attgaatttg agaatggatg tgttgcaaac cttactgcca gccgcatttc catgaaaaac
    12601 atgcgtaaag caagaatctt tcagggcgat tcctatattg ccgttgattt tctgaaaaag
    12661 aaaacagaaa taatctccat gcgcaataca tcagagaatg aaagcccttt tgccataatt
    12721 attgatcccg gaaatggtaa gccaaacaaa acaattgagt ttaacaatcc tgttgtagaa
    12781 gaaagtaatg ccataaaaga agagctaaga acctttgctc ttgcagtaga aaacaacacc
    12841 actcctatcg tgaacgcaat ggatggctac aaagcattaa aggttgccaa tcagattatg
    12901 gataaattgt cgcagcaaaa attcacagag agatgatgag gcttaaacaa cttttcaccg
    12961 tgattgcatt tgcatcatta atgcaagcgt gtaatctcat taatcctgca gaaccaacac
    13021 ctgcctatat tcagattgac acaatcaatc taacaacaaa tttaactaca caaggaagtg
    13081 catctcataa aatagttgat gcctgggtat atgtggacaa tcaggcttta ggtgcttatg
    13141 aaatgccttg taagtttccg gtcttagcaa caggtgaaca tactatatat atacaaggtg
    13201 gcattttact gaatggcatc aatgccacac acctgcaata tccttactat aaatttgttg
    13261 acaagacagt aaccttaaag cccggagaag ttgtcaaaat tgataacctg tcaacaagtt
    13321 atattgacaa tcttactttt ccattaatgg aagatttttc aggattgttt tcattcgaaa
    13381 aaagtggtaa tagtcccgtt ggtatgcttg gcgagtttga tgcatcgcaa ggtatggaag
    13441 gtgggtatgg tgttgtacgg ttggattcag gcatttataa ttttgaagta aaatctaaag
    13501 aaatgattct gcccaacaat ggcacaaatg tttttttaga gatgaattat aaaaactctg
    13561 ctccttttct tgtaattctt caggctactg atccgtttgg cactgtaatc aacaacgcaa
    13621 ttatcacatt aaatacaaaa acagagtgga ataaaattta tattgaacta aaaccaacaa
    13681 tgcagttaac cagcacatcc aagcgtttca gagtgctttt tggaatgcaa cgtaccgatg
    13741 caagtacaaa tgagagtttt tacttcgaca atgttaaggt agttcataat taagaattgt
    13801 tgttgaaggt atagcaatga ataaacggtc acaagtcatc aaatatatta ttgccgattt
    13861 tctgtctgca ttgctggcct ggactatgtt tttcatttat cgcaaaacat gtattgagcc
    13921 cggtgaatca ccttttacta aggaatttct gtcggatcca aaattttatt ccgggatttt
    13981 tattttacct gtatgctggg ttctatttta tgcatttctg ggcacctata ataatatcta
    14041 tcgcaaatca cgcttacgcg atctggcaca aacagcactt gcatctttaa ttggtgttac
    14101 aatcatattt tttattttac tgttggatga tgtcattgtt acctataaaa attattacaa
    14161 cacttttctc acattactca tcattcatta tagcattacc gtatttttca ggttaataat
    14221 cacaacgatt acaggaaaca aaatcaaaaa gaggaaaatt ggattcaaca ccttattggt
    14281 tggcagcagt cagaatgcct tacatatttt tgaagaatta gaaacacaaa aaatcagtca
    14341 gggcaataaa tttgttgggt ttattcatgt tgatgaaaag aatggtgaat atttgggagg
    14401 aaaactccca caccttggtg gcattgctga tattcctgaa gctatcaata aacatctggt
    14461 agaggaagtt atcattgcaa ttgaatcatc tgaacatgaa aatattggta gaatcattaa
    14521 tgaactggaa gacactaatg ccatcattaa aattatcccc gacatgtatg atattctttc
    14581 aggctcagta aaaatgaatt ctatttttgg agcaccgctg attgaaattt atccgcaact
    14641 gatgccagca tggcagcagt atgctaaacg actgatggat atagttgtgt cttttatttt
    14701 tttagtcttg ctaacaccaa catttattat tactgccctc attgtaaaac ttacaagcaa
    14761 aggacctgta attttcaaac aagagcggat tggacttcat ggtaatcctt tcaacatcta
    14821 taaattccgc agcatgtatc ttgatgcaga atccggtggc cctaaacttt ccagtaaaga
    14881 cgatagcaga attacacctt ttgggaaaat tatgcgtaag ttcaggattg atgaaacacc
    14941 acaatttttc aatgttctca ttggtgaaat gtcgcttgtt ggtccacgtc ccgaaaggca
    15001 atactttatt gaccagatca tgcttcattc accgcattac agacacttac ataaagttcg
    15061 tcccggaatt acatcatggg gtcaggtaaa attcggctat gcagaaaatg ttggtcagat
    15121 ggttgaacga ctaaagtatg atatcattta tattgaaaac atgtcgctgg cacttgactt
    15181 taaaatactt ttttacactg taaaaattat ttttcaggga cgcggcaaat aaggctatca
    15241 aattaatatc aaaaaaaata ttttcaaatt acaaacctta aagaatacat ttcatgataa
    15301 aacaaattgc agtaatagga tcgggtacaa tgggaaatgg catagcacat gtttgtgcac
    15361 aatatggaaa caacgtttcg ctgatagaca tttctgaagc acctttagaa aaagccatca
    15421 agaccattac aggaaacttc gaccgaatgg taactaaggg gatagtgact gctgcagata
    15481 aagatgcggc attacaacgc ataactactt acacgaattt atctgatggc gtaaaaaatg
    15541 ctgaccttat tattgaagca gcttcagaaa acgagagtat aaaactcagc atttttaaag
    15601 aattagacag cgtttgtaaa gccgatgcta ttttagcaac aaacacttct tcaatctcta
    15661 ttacaaaaat tgcatctgta accaaacgtc cagataaagt aattggaatg cattttatga
    15721 atcctgtgcc tgtgatgaaa cttattgaag ttattcgcgg atacaaaaca agtgatgaag
    15781 taacaaaaat aattttcgac ttgtctgtta aactcaataa gattcctgtt gaggtaaatg
    15841 actatcccgg ttttattgcc aacagaattt taatgcctat gattaatgaa gccatctatt
    15901 cactttacga aggtgttgcc ggagtgcatg aaattgatga ggtaatgaaa ttaggtatgg
    15961 cacatcctat ggggccattg cagttggccg atttcatagg ccttgatgtt tgccttgcta
    16021 ttttacgcgt attacatgat ggttttggaa atcctaaata tgcaccatgc cccctattag
    16081 taaatatggt tacagccggt aacttaggta aaaaatcagg agttggtttt tatgattacg
    16141 catcaggtgg aaaagacctt atcgtagctg caacatttca aaaataaatg gcactaccct
    16201 atcctgaaat cagactaaaa aaaggaaaag aaaaaagtgt tttaaatttc catccctgga
    16261 ttttttcagg tgcattggag aaaatgcctg cacaactgaa taatggtgat acagtgacac
    16321 tactttcaca tgatggtgag atacttggca caggcctgtt tcatcattca agtattgcag
    16381 taagacttct tgctttttct aaggttgagt tgaatgtaac attctggatt caaaaattat
    16441 ccaatgcctt acagcttagg aaaaacatac acttgtttaa caataaagaa accactgcat
    16501 atcgtttggt gcatggtgaa ggtgatgggc tttcgggatt aatcattgac atttatggtg
    16561 actgcgcagt gatacaatgt cacataaaag gaatgttcag ccatcgttat ataatagctg
    16621 aagcattaaa taatgttttc aatcaatcaa taaacacaat ttatgataag agcgaagatt
    16681 ctttttttga aaataatgaa agcagatttc tcaaaggaga aaagcaatct gaaataataa
    16741 aagaaaacgg acatcggttt tatgtaaact ggtttgaagg tcagaaaaca ggatttttta
    16801 ttgatcagcg cgagaacaga agattgttga gtaattattg tgacagtaaa aatgtaatca
    16861 acctatttgc ttattcaggc ggtttctcca tatatgcatt aaagtcaggt gctgctttgg
    16921 ttcattctgt tgacagttca agtcgggcag aaaattgggc aaaccagaat gttcaactaa
    16981 acgatgcagt taatcatcaa tttttttgtg aagatgtttt tacctttttg aaagaaacaa
    17041 aaaacaatta tcaggtgtgg gtagttgacc caccagcatt tgccaagcgt cttgatgccg
    17101 tgcggaatgc catgataggc tatcggaatc tgaacactgc tgtatttcgc aaagcatctc
    17161 ccggaagtat catattcaca ttctcctgct ctcaggctgt tgatgcgtta accttcagga
    17221 aagtaatttt tcagagtgcc aaagaagcac agaggcagat aaaagttttg catatacttt
    17281 ctcaacctgc cgaccatcct gtttcagttt ttcatcccga aggtgaatac ctaaaaggat
    17341 tagttttgta tgttgaataa cactcattta atttaaacag attatttcaa tatgcttcag
    17401 aatataccac agttaaaaca tacagcaaca ggaaattttt ttctgatggc cggaccttgc
    17461 attgttgaat cggagcagat gtgtatggac attgcaggaa cagtaaaaga aattacagac
    17521 agacttcaaa ttccatatat ttttaaagca agtttcagaa aagctaaccg ttccagactt
    17581 gattcattta ccggcattgg cgatatgcct tcgctcgaaa tactgcgcaa agtccgtcag
    17641 cgttatcaat tacctattgt taccgatatt cacgaaagtc atgaggctgc acttgcagca
    17701 gattatgttg atgtactaca gattccagca tttctttgtc gccagacaga attgttggta
    17761 gcagcagcac gaaccggaaa aactgttaac gtaaaaaaag gacaattcct ttctcccgaa
    17821 tctatgaaat ttgccataga taaaatacga caatccggta accatcaggt aatggttact
    17881 gaacgcggca ctatgcttgg ttatggcgat ttggtggttg acatgcgcgg cattcctgag
    17941 atgaaaaaat ttgatgtacc ggttgtcatg gatgtcacgc attcattgca acagcctaat
    18001 caggcatccg gagtaacagg aggtaaacct gaacttatca gcactattgc ccgtgctgca
    18061 atggctgccg gtgccgatgg tatttttatt gaaacacatc caaatcctgc caaagcattg
    18121 agtgatggtg ccaatatgtt acagcttgac ctccttgaaa agcttttagt ttcattgcta
    18181 aaagtgcaac aagcagttcg ttgatatttg atgattgatt aatactttgt cttgaagttt
    18241 ttttcagaac tttgatttgg acaattgtat tattatttca taccttacat cgccatttac
    18301 acaatacatt taaaaaaatt gacaagtgag tgtaaagcaa tgaacatatc aaaacagaaa
    18361 caatgaacag caagctgatt atatttattg tcatatcagc aattcatctt attgcacacc
    18421 taatggcctt tcagtggttg gtatttatca ccaaaccatt actgatgccc atattgcttt
    18481 ggtatttttc agaagggtta actttcggca aactgacact tatccaaaag cgtatgatta
    18541 cagcactcac actggcatgg attggcgata ttataaatct ttacagtcat atcaatcact
    18601 ggatatttac agccggtata attatttttc ttgcaatgca ggttatgtat atcattattt
    18661 gtttttatct cattcgcttt aaacaaatca aacagctata cctcatcttc tgcaccttag
    18721 tatttattgc ttatggcagt gtgctgctct ggttattact accacatctg gatgaaatga
    18781 ccatcccggt tttaatttat gcatctgcgc ttaccttaac cggaattgct tcagccttaa
    18841 caaaagatca actgccgcga aaggtttttt attgcttatt aactggcaca ttgctttata
    18901 taattactga cagtttggat gccatcaata attttagagc atctttccct tatgctgcat
    18961 tctggattgc actcacctat ctttcagcac aaatgatgat aaccattgca gtaaagaaaa
    19021 ttaaaactaa ttagttaatc tctaattctt aattatcaac tttctataag acctcgtccg
    19081 gttttcttta ctttgtcttc tttgcgcagt tcttcaacaa tcttatccaa tacaccatta
    19141 ataaaaacct tgctctgtgg tgtactataa tctttggaaa tatcaatata ttcgtttagg
    19201 gtaactttta aaggtatctg atcaaatttc aatacttcgg taacagccat tttaagcata
    19261 atcatatcgc ttaaggcaat acggtcaact tcccagtttt tagtacgttc tgcaattatt
    19321 gatgtccact ctttatcatt cttaatggtt tccttcagaa gtctgatcat aaaatcacgg
    19381 tcatcggcct cgtctttaaa cagcggtaaa attgcaggtt gatctgtagt ataaaattgt
    19441 ctgattgttt taatcaccat aacatgagcc aactccaggc tctctgccca ccagatgttt
    19501 ttttcttcaa ttacaccatt gatagattcg gattgttgta cttcttcttt atacaatttc
    19561 aaaagccaat ccatttctac aacttcagta ttggtcatag taaagtcacg gtatgacgga
    19621 tgctgcctta atttataaaa acattttcca accgcttcca tatcggcctg ccaactgatt
    19681 ttatgtttct tgcaattatc tataaactgt tcatcgtttt tcaaccatga tataaatgta
    19741 gtcttttcaa aagagcgtat aaactctctt ttattgacaa taaactttga tgctacatca
    19801 atgcgataca actgctcctg ctccgcaagt tcaatcaaca attgcaaaag ggtaaggtaa
    19861 agttcataaa ggcggctcgt tccttcaata agttcttttt cagctttggt cacattatat
    19921 ccttcacttt gataataagt atataatacc tgcaataccc gaatccttaa atgacgtctg
    19981 gctaacataa attattaaat ctaataagaa cgaaaaaagt ttttgtttac tataaattat
    20041 ttacctccat aaatacgttt ctctgccatt tgcatagcag ccaccaatgt agggattttc
    20101 tcagcttttg actttttgaa aatctctaag gtaacgttgt atatatgttc ggtctgtttt
    20161 aaagcttctg cacgactgaa tttcttcaac tcgctgtaaa cattaataag tccgcctgca
    20221 ttgattaaaa agtcaggcgc ccataatatg cctttgtcca aaagcatttt gctatgaaca
    20281 acttcgtcag ctaactgatt atttgctgca cctgatacaa tagcacattt aagtttacca
    20341 atattttcag tattcaacgt tgcacctaat gcacatggtg cataaatatc aacatctaaa
    20401 gaaaaaatac tgtctggagc aactacttct gctttataag ttgttgctac ttgttgcaat
    20461 gcatctttgt taatgtcggt aacatacacc ttggcacctt cttctgtaag gtgttttact
    20521 aagttactgc caacatgacc tgcaccctgc acacaaactt ttttaccgct caggctgtca
    20581 tttccccaca cctctttcgc tgaagctttc atgcccatat aaacaccata ggcagtcaca
    20641 ggtgatgggt cgccactgcc gcccattgac tcaggcaaac cggttacatg cttggtttct
    20701 ttggaaatgt attccatatc ttttgtagat gtacccacat cttcggcagt gatgtacctg
    20761 ccacttaaag agttcacata ctgtccaaac tttctgaaca aggcttctgt tttatctttc
    20821 ttgctatcac caataataac agcttttcct ccacctaagt taagtcctga aattgcagct
    20881 ttataagtca ttccgcgcga aagtcgcaga acatctgtca aggcatcgtt ttcattctga
    20941 tacatccaca tacgagttcc gccaagtgca ggacccaaat tggtattgtg tatggcaata
    21001 attgccttta aaccggtttc atgatcataa caaaacacca cctgttcgtg tttcatatca
    21061 gtaacttgtg caaaaacact attggcagtt gctattgttt ctttcatttc ttttacttca
    21121 atcatttaca atgtttttat ctgattgtta attcagttta atttgcaacc ccaacgacct
    21181 gcaaacaggt cgcaaaagta gttttttttt gctctgaaca aaaatttcat ttacatgtcc
    21241 gacttattaa cattaaatcg ctttttcgta aaataccggg gtcggcttgc tcttgggctc
    21301 atttttgtac tctttgccaa ctatttcggc cttttacctg cacaaataac ccgaaaagca
    21361 atagatttta tctccaccgg aacacaccat aattattccg attcggccaa aactttaaca
    21421 ctttacgcat tgggcatttt tggcgtggtt ttcctgcgtg gcgtattgct atttctgatg
    21481 cgtcagacca tcatagtcat gtcgcgccac attgagtttg atatgaaaaa tgagatttat
    21541 gatcagtatc agaaacttga tatgcacttt tatgcacgtg ccaatacagg cgacctgatg
    21601 aacagaatca gtgaagatgt gagcagggta cgtatgtaca ccggcccagc aattatgtat
    21661 ttggtcaata ttaccgtgat gattattgta gtttttatag ctatgatgaa catcagtccg
    21721 ggtttaacct tagtggtatt attcccattg ccactgcttg ttatcctgat ttattatgtg
    21781 cagaaaatta tcaacaccaa gagtgaaaaa gttcaggaag cattaagtat gctttccacc
    21841 aatgtacagg aatcttttgc aggaatacgg atagttaaat ctttttctaa agaaaaagat
    21901 tttgcagctt tatttcatac tcaggcaaaa aactatttca atcagactat gcaactggca
    21961 cgtatcaatg cattgtttat tccggccatc atgttgcttg tgggcattag ctctgttact
    22021 gtaatttact atggcggaat tttggttgat gaaaagcaaa tcacctatgg aaatattgcg
    22081 gagtttgtta tttatctcaa tatgctcatg tggcctgtag gtatgttggg gtggataatt
    22141 acactcatcc aaagagcaga tgccagtcag cgaagaatta atagtttctt acatacagag
    22201 tctgaaatca aatacaacaa cacaggaata gttccagaaa atgtaaaatc catagaattg
    22261 aaaaatgttt cattttcatt cggaaaaaac agtgatcatg cattggaaaa tattaatctg
    22321 caactctcaa caggaaaagt tatagctata gttggcacta caggctccgg taaaactaca
    22381 attgccaatc tggtatcaag actctatgat ccaacacaag gagaagtatt ggttaacgat
    22441 attccactta aaaacatgaa ccttgaatgg tggcgcaggc aaattggatt tatcactcag
    22501 gatgtgcttt tattttctga ttccgttgcc aataatattg catttggaac aaaagaatat
    22561 gatacagaat cattgacagc tgctgccaaa acagctatgg tttacgatga aatagttcat
    22621 tttaaagatg gcttcagtac catgttaggt gaacgaggtg ttaatcttag tggaggacag
    22681 aaacaacgta tttccattgc acgcgccatt atgaaacagc cacagttttt gattcttgac
    22741 gattgccttt ctgcactcga caccggcaca gaagaacagc tgctgcaaaa cttgttacct
    22801 tattttcaac atcgcataac attaatttta gggcatcgca tttcatcagt aaaacatgcc
    22861 gatgaaataa ttgtcttgca tcagggtaaa atactggaac gtggcacaca tctgtcacta
    22921 ctgcaacaaa atggcttcta tgcaaaaatg gttactgagc aacttaaaaa gcaggccgtc
    22981 aaatcctgat ttccggtaaa catccattct gcttttaaac attgtttgca taaataaagc
    23041 tgattgattt tatttgccat cggatgaatg catatatagc catacattgc aaggtagaac
    23101 ctgttcagcc attcagtgaa atactaatag cccaacttgc tgaaattggt tttgaaatgt
    23161 ttgaagaaaa ggaagatgga tttgatggat ttatcaggaa agaaaatttt aatgagtctt
    23221 tattagaaac aatagagttt ttgcagccaa acgagttttg cactgcacat tggcagttag
    23281 aagaagtaga aagtcaaaac tggaatgaag aatgggaaaa aaattacccg ccaataagag
    23341 ttaaaaatat ttatgtacgt gctccatttc atcctgatgc atcagacaat gatcttgaaa
    23401 ttatcattca gccaaaaatg gcatttggca caggacatca tgcaacaact gctatgatga
    23461 tggaattgat gctggaaaca aacattgctg ataaaaatgt tttagatatg ggatgcggtt
    23521 ccggcattct tgcaatactt gcctcaaaac ttggttgcac ttctgcaacc ggcatagata
    23581 acgacccgaa ttgtgtactg aacagcattg aaaatcttga aaacaataaa ataagaaatg
    23641 ttgaagtact tcagggagat gcctcgtcac tcaaagattt aaaattcgaa attattcttg
    23701 ccaatataaa ccgtaatatt ttattagatg atattgcatc gtataataat gcattgtctg
    23761 atggtggaca gttgttggtg agcggttttc tgacagaaga tgttgatcgc atcaaacaat
    23821 cgtttggaaa atatggtttg aaactttcac gtatgaaaca aaatcagaac tgggtagcca
    23881 tgcatttttt caaatcccat tccggtaaat gacgcatcat aaagaaacta aaaacccgct
    23941 aaatattatt gtcatagttg ctgcattagg ctattttgtt gacatctatg atttgatttt
    24001 gtttggcatt gtgcgtactc caagtctaaa tggtctgggt attaccgagc ctgatttagt
    24061 tactgagtat ggcttgcacc ttttaagtta tcagatgatt ggtatgctta caggtggact
    24121 tctctgggga attatgggtg acaaaaaagg acgtgtttcc gttttgtttg gctctattat
    24181 catgtactct attgcaaata ttgctaatgc ttttgtacaa gagatatcag attatacttc
    24241 gtcatcaata ataaatacat atactgtttt gcggtttatt gccggagtgg gcttggcagg
    24301 agaactgggt gccggaataa ctttggtaaa cgaaaccatg actaaagaac atcgtgggta
    24361 tggaaccatg attgtagttt cctttggtgt gttgggggca gtctttgcaa atattcttgg
    24421 gcactttgta acgtggcaat cgtcatacat tattggtggt gtaatgggtt tggttttact
    24481 gtttctgaga attggtgttt atgaaagtgg catgtttaaa aatcttcagg tgcatagcct
    24541 tgaacgcggt aatctgatga tgttattcaa taacagaaaa agattcttaa cctatttgag
    24601 tagtattgca gttggtgttc ctatctggta tatgattgga attttagtag tcttttcgcc
    24661 agaaattgca aaaatgaatg gtatcaccgg tgttaatggc ggtgatgcca tcatgtattt
    24721 ttatttagca acctgcgtgg gcgattttct gagtgcctat tggagtcagc aatggaaaag
    24781 ccgtaaaaag gttttgaaac tttatctgat aatcacgatg gtcattacac ctgtttacct
    24841 ttatctaatg ggcaatgcct ccgtgtctat tttttatgct gtatgcgggt tattgggttt
    24901 tgcatccggc tattgggcag tgtttgtaag cacagcttca gaacagtttg gtaccaacat
    24961 cagggctact gttactacaa cagttcccaa tatggtcaga ggatcactag ccattttaac
    25021 cttgcttctg gcctattttc gtgattcatt aggattgaac atgattgatg ccatgtttgc
    25081 tgtaggcgtt attactgctg ccggagcatt tttatctttg cttggtatca aagaaagtta
    25141 tgccaaagat ttaaattata ttgaaacatg aaaaagaaaa cattggtgat gggcgcttca
    25201 cccaacccgg cccgctattc taataaggcc attcacaaac tcctgtacta caaccaccct
    25261 gttgtggcca ttggtgccag gcctgataaa gttgaagagg tagtaattga aaccggacaa
    25321 attgccttta aaggcattga taccgtaaca ttatatctta acgaggaaag gcaacatccc
    25381 ttagaagatt acattttgtc cctgcatccc aaacgaatca tttttaatcc gggagctgag
    25441 aatgaatcat taaaaatcaa ggcagaaaaa caaggtattg aatgtgtgga agcatgcacc
    25501 ttagtgatgc tcactattgg cgattactga ttctcgttaa ttctaacagg ctctttcagc
    25561 tctttctttc tgtcgccacc ttcggtgcat gcacctactt ttttaataca acgtggactt
    25621 tttctttgtt gcatgtttga attaccttta tcatgcgcat cattcgactt atgaatcatg
    25681 attacaggag tattgttaat ctctctaacc tctgtaggac gtccaccggt ttttattggg
    25741 cgagcctgat cttgaccaaa tgtgctctga gtcaaaaata aacaggatga aaataaaacc
    25801 agattgtaaa tattcatttc ttttgttgta atggaagcaa atttacgaca ttaccggtga
    25861 aatttatgtg aatgaagata ccggaaattc tactctgaaa gttgtgcctt tgttggcttc
    25921 cgaacttacg ctcaatgtta aacgatgaaa atcagaaatg gttttaacaa tagccaatcc
    25981 taaaccgaaa cttggttctg ttgatttatt aaatttctta aatcggtcga aaatatgtga
    26041 gagattttct tttacaatac caacaccggt atctctgatt tcaaccacaa aaacatcatt
    26101 ttgtttagaa gcacttatat ctatctgccc attctccaca ttatacttta tagcattagt
    26161 gataatgtta ctaaagagtg taaaaagtaa agttctgttg gcattcggaa attcaaaatc
    26221 atcaagatgc tgcatgtaga gcctgatgtt cttagtgctt aaatgttcct gaaaatcttc
    26281 aacaatttcc tgaagcagtg ctttaatatt tacggtttca tttttcaaaa actgttcatt
    26341 atcaattttt gaaatcatca gtaatgactt gatgatttta ctcattctgt tcaactttct
    26401 tattgaatca tccagcttat cagctacatc atcatcaatc ttttcatcca taagcatatt
    26461 ctcaaaccgt gatttgagaa tagaaatagg tgtctgcaac tcatgcgaaa cattcgaaat
    26521 aaattccttt tcggtgagga aagcactctt tatacgctgc atcagctctg ttaaactgat
    26581 gtctaactgc tgaaattcaa gcgtagtagt ttgaaccggc acatcattga aatgtacagg
    26641 gtcttttgtt ccttttaact ttcgctccac aatttcattc aatgggcgca gcaacagtcg
    26701 ggcataacca acatctacaa aaatggaggc tataattgtt aaaatagttg cccacaaagc
    26761 tactctgcca atagtatttt ttagcttgtt taaagaatct attccctctc ctatctgcaa
    26821 actataaatc tgattgtcgt aaataaatga ctgacggatt atgcgatgat ttaacttttc
    26881 gccatcaatt tcccattctt cattggtaat tactgtgggc tttacatctt tcattcccga
    26941 cagtggctct atgaggataa actcttcttt aagaatattg taactttcaa aagtacagtc
    27001 cgattctctg gcaatgtcat tcagcccacc tttttcaatt atgcgcaata cttttgatgt
    27061 acgcgctttt aacctctggt caatgtgatt ataaacaacc ttctctacaa taaaaggcat
    27121 taatgccccg aatgccagca ccacaagtgc tttactcagg gcattataga tagcaagttt
    27181 taactggagt ttcatctcca gccaaagtta gtatttatca ttgggcattt aacctatagc
    27241 caataccacg aactgtttca aaccactcaa taggtgcatg agcagctaat tttttacgga
    27301 ggtttttaac gtgaacatca atatagtttg aatcgtagtc atcatccata acatcgcccc
    27361 agatgtgttc tgttaattga gatcgtgata aaacacgtcc cttatgtaat gccagataat
    27421 gcaataagtc aaactctttc ttggtcaata caacaggcgt ttcttcgtat gtcagtgtgc
    27481 ggttctgata gtccatcatg aaaccattga tgtcaattat ttgtacttta agtccatgtt
    27541 ttcttcttaa aatagcctgc atgcgcgcat gtaactcagg cagtgaaaat ggtttagcta
    27601 agtagtcatc agcacctaaa tccagaccca gaattttatc atctgtttca cttcgtgctg
    27661 tcagtacaat gaatgcactg tctatagcgt ttttcttggc ttctttcaaa atttcaaagc
    27721 cattggtatc gggtaaacca atatccaata aaacaaaatc gtaggagttt acccacatca
    27781 tttctaatgc cttggcacca gtaaatgcta catcgcaatg cagaccttgt tttgtaaggt
    27841 aaatttccat ttcatgagac aaactacgtt cgtcttcaac aattaataca ttcataattt
    27901 ctgtgttttg aaaggttaaa aataaaaaac ttcatgtaca tgaagacaaa aatactgccg
    27961 aaatatgaag aaaccgatta at
//
LOCUS       DAOQNP010000034        27871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_554_length_27871_cov_11.510965, whole genome shotgun sequence.
ACCESSION   DAOQNP010000034 DAOQNP010000000
VERSION     DAOQNP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27871
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_27871_cov_11.510965"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4459
                     /locus_tag="PKY09_06335"
     CDS             <1..4459
                     /locus_tag="PKY09_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12511.1"
                     /translation="TISGPVVTVDSVKNARCFSQANGAVYISVTGNNGPVTYLWSNAA
                     TTQDIVNIAAGTYTVTVTDSTGCVGNKTQIITQPSVLTLIDTIVPARCGNANGSAGVN
                     VTGGTSPYTYLWSNGASTSSISGLNGGTYTVTVTDSKLCTANRTLVVNNTPSLVISID
                     TIIHVKCAGQNTGVIKVNVTGGSGALTYTWTPSQIGTDSIFNLASGTYKLVVRDTANC
                     RDSISVTINSPTAINATINSLPETCGINNGQAWVTVSGGTPGYTFLWNNSSTNDTITN
                     LTKGNYSVTIKDANNCSVIKNILVDSLGGPAITDSIIHVKCFGENTGAIYTTVHGANG
                     PVSYLWSPGGQITQNITNLTAGTYTVTVTDTNSCSSTKSFVVNQPTGLNISFTSVTEK
                     CNQANGSAIVAVTGGTSPYSYQWNNGNTTNNIINLTSGYYTITITDGNLCTKRDSVFI
                     GHTNGPSISNFSHNLIKCHNDSSGSLTVNVTGGTPFYNYQWSGFPLVNNATLQNIPAG
                     VYTVTVTDANGCTVNATDSLQNPPLMSISHTLTNASCNLNNGIAQINVSGGASPYIYN
                     WSNGVTGATLLDTLANSFYTITITDALGCIKTDTFTIQRTPATFIQLTSLNYVTCFGL
                     SNGSITITPSGGDGNYTYQWSYNNIQTQNLNNIPAGSYDITVTDGTGCSYTMPDIQVS
                     QPDTLIAQVTTSPTGCTSPTGSATANQTGGIQPYTYLWSTNATSQTINGLSSQIITVT
                     ITDNNGCTATNSGIISALSAPVVNISGTQNINCFGGTNGFINTTVTGGNQPYNYLWNN
                     GSTTSNINNLSAGTYTLTVTDSAQCSTIITQTLTENPKIQFGLSLIAGPCNGGTPAIA
                     ISAPSGGVAPLNLLWSNGSTSTSAQATAGTVMTLTITDNAGCSRDTTFSMLSTNGPQI
                     SSIQTTDIVCFGDSSGSATVNISGGTPPINIQWIGTLPVQTTATAINLKAGSYTCIVT
                     DVNGCSAQSVANINHLNSLITPTLTITNASCGNSNGQIVVNATGGGGTYQYKLNNGVY
                     SSTNTFANLSAGSDSVFVKDNNGCEVFVISTISNIQGPVISVIDSANVSCFGSNDGFI
                     ILNVTGAAQPITYSWSDGPTTLTRNNLGPGNYTFTAQDANNCISVRNFIITEPTTITI
                     SEIIPNKNGVYNITCNTLADGEIQVIANGGVPFTQTSAYNYQWSISGASGASVTNLTA
                     GPYTVTVTDSMGCSATKSFLLTEPPALNAGADFTQSICGTDTIVLIGNIPSYGFGKWT
                     VRSGSGIIADPDSITTQITAIANGVNVFAWVVYDNFCSDTALYVINKKEKIVADGGID
                     QNVCDNFVFLSAVPPPSGTGVWQSINGNSTIENPSSPATKVENLSPGKNIFVWTITNG
                     TCTASDTVKIAILPPEECFTDVVMPTGFTPNEDGYNDLYVIRGLDNTNNTLTIFNRWG
                     NLVYEKKNYNNDWDGKSNNKQILPDGTYYAILRIPARNLVLKTYIDLRR"
     gene            4464..5384
                     /locus_tag="PKY09_06340"
     CDS             4464..5384
                     /locus_tag="PKY09_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNB12512.1"
                     /translation="MKKLCSIIIMFILSDYLFAQQAPMTTQYMFNTLLINPAYAGSKP
                     FTSATMMVRKQWAGFKGSPTTETASIHGALEDKNVGLGLYISNDHAGIINRTELFGSY
                     SYTIEYGESKLAFGLQAGLSYLKSTLSDLVYNDNNDPVYEKNTINNVLPNFGAGLYYH
                     TDLFYAGISAPLLLSYDPLKSLSLEMTDVHHIRRHYFLTTGYVFSLSEQVKLKPSVLL
                     KYVDGAPLQFDINANLLLNNIIWLGASYRSNDAIAVIGEYQLSKKLRIGYSFDYSLTL
                     LRNHSAGSHEFMLGYDFGYNVPKIKTPRYF"
     gene            5387..7267
                     /locus_tag="PKY09_06345"
     CDS             5387..7267
                     /locus_tag="PKY09_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB12513.1"
                     /translation="MNRKRNIFLLFVILFFTSCLSVKQRTADTYYEQNLYDKAIPYYQ
                     EVTNKKFNLDILIKLADCYRNVKNYALSEQIYSKIVTSNTDPIYQFYYAEALMQNGKY
                     VEARKWLNKFLVLNRADSRALNLLASCDSIEMLYKDSLLFDVRRLPFNGIDESNFSPE
                     LFHSGIVYVSNRNTDPKNYKTNPVIDNYNLFYAQQTELGHWMDPVPLRGTINSIFNEG
                     PCTFNNEFTKAYFTRNANEGKKIVTNQQSENVLEIFEATTKDGEWNVSGPVSFNNNNY
                     SVGHPSISPSGNTMVFVSDMPWGYGGTDIYRVQWINNTWSKPASIGKVINTSGNEMFP
                     FLLNDSILYFSSNGMIGVGGLDIYRTELTADGWTVPENLGYPINSPRDDFGLICDNEE
                     LSGYFSSNRQQGKDNIFSFKRNAPVLTLKGKLIDKSTNKPLKNINLKLTANSIDTILT
                     TDGEGNFTAQLKNNKDYTLRAEDKSYFTTSENISTFGYRKSATLNKDLTLEKVIFNKP
                     VIWRGIAFEKGKSDLTPSTKTELNKLFNILLENKNIKIELSCHTDSRGVERDNLILSQ
                     ARADKAAAYLINKGISKDRIISVGFGSQRLLNNCVRGILCLEEDHQINIRTEIRYSPY
                     QR"
     gene            7301..9100
                     /locus_tag="PKY09_06350"
     CDS             7301..9100
                     /locus_tag="PKY09_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNB12514.1"
                     /translation="MANVLGISAFYHDSAATIAIDGKIVAAVQEERFSRIKNDAKFPV
                     NAIKYCLQYSGLKLNQIDHIVYYEKPFLKLERLLETYLETAPNGFQQFTQAVPLWIKE
                     KVLIKNKIRENLNLIDENFSAKEILFCEHHLSHAASALYTSPFSNAAIITADGVGEWC
                     TTSIMNGTPNTITSLKELNFPHSIGLLYSAFTQFLGFKVNSGEYKVMGLAPYGNKNSS
                     QFSFFKNKISELFDLKSDGSVFLNIRYFNFQKGLSMINENLWAELFEIKPRIAESEIN
                     LNHCHLALAIQTITEDVIFNLAKQAKQITNADNLCLAGGVALNCVAVGKLNKQNIFNK
                     IYIQPASGDAGGAIGAALAIEHIFLGNKRKTPEKDHLQGCRLGPAYDNQTVAQSLTKY
                     NLHFEQFKEEELLKLTATHLSNNKTVAWFQGRMEFGPRALGSRSILANPSTSNIQQTL
                     NQKIKKRESFRPFAPAMLIEDYLEFYEGGNENGFMSMTDFLKKEKRMGNDSTPEEAGI
                     TEQLQKSKSAIQGVTHVDYSSRIQVVTETDDSLFFKLLTEYKKITGRGILINTSFNLR
                     GQPIVCNPDDACKCFLETEIDLLAINNFIVRKK"
     gene            complement(9123..10445)
                     /locus_tag="PKY09_06355"
     CDS             complement(9123..10445)
                     /locus_tag="PKY09_06355"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HNB12515.1"
                     /translation="MDNIKEKFKEKALPMAGTIKDIVKTHGNFVLGQYTVEQVYQGMK
                     GMIGMVTETSKLDANEGIRFRGYSIPELREKLPVSKEGPEPLPEGIFYLLLTGEMPLL
                     KDVIEVNNEWMKRATVPKHVFDMLDMLPEKTHPMTQFSMAIMALQTESVFARKYREGM
                     NKKDYWDPMYEDVMNLIARLPRIAAYIYRSTYKEGKHIEPDPKLDWAGNFAHMLGFEE
                     LDFRKLMRLYLTIHADHEGGNVSAHATHLVGSALSDPYLSLAAGMNGLAGPLHGLAAQ
                     EVARWIIELRDKYNGVPSREQLGEFIKTTLTEGKVVPGYGHAVLRKTDPRYTAQQDFA
                     KKYMADDELFKIVQLLYDVTPEILKATGKIKNPWPNVDAHSGILLMHYGLVEYDFYTV
                     LFGVSRAIGVLTSLLWDRALGMPIERPSSVTTQWIQEKIKSGEAVKVE"
     gene            complement(10477..10746)
                     /locus_tag="PKY09_06360"
     CDS             complement(10477..10746)
                     /locus_tag="PKY09_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12516.1"
                     /translation="MELEESDGEIKKERIRIRKRIRIKKKPDPKKKIKKYLRIFAWII
                     VVAIFITTLIVLFSESDLRYENTKKPKSRVEIIPSTKDFSVNFNL"
     gene            complement(10792..12081)
                     /gene="eno"
                     /locus_tag="PKY09_06365"
     CDS             complement(10792..12081)
                     /gene="eno"
                     /locus_tag="PKY09_06365"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNB12517.1"
                     /translation="MGNIAEIRARQILDSRGNPTIEVDVYTDNGVVGRAAVPSGASTG
                     AHEAVELRDNDKKIYLGKSVMRAVNNVNAILNKELHGYPVFDQRSIDAAMIHIDGSPN
                     KATIGANAILGVSLACARAAASESGYPLYRYIGGVNARILPVPMMNILNGGSHADNSI
                     DFQEFMIMPVGAESFSESLRMGVEIFHNLKSVLKSKKYSTNVGDEGGFAPDIKSNEEA
                     ILTVIEAIEKAGYKPGDDVMIAMDAAASEFYDAENKVYHFKKSSGEKLTSSEMVSYWK
                     QWCEKYPIVSIEDGLAEDDWAGWKELTNVLGGKVQLVGDDLFVTNTERLARGIKESIA
                     NSILVKVNQIGTLTETINAVEMAKNNSYTSVMSHRSGETEDTTIADLAVALNTGQIKT
                     GSASRSDRIAKYNQLLRIEEMLGGAAEFPGKNFRNLI"
     gene            12190..12939
                     /locus_tag="PKY09_06370"
     CDS             12190..12939
                     /locus_tag="PKY09_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB12518.1"
                     /translation="MKVLKIAFGLYAAIVFFITLLCVFPLYFIVFLFFSERKSPVIAH
                     NISRFWALVLFRSFFIRYKIINEDFISDKGTYVFVANHRSMLDIPLYARACSNTFRFL
                     SKAELTKIPLLGYVIRKLYVTVKRGDKKDRQKSIEAMRKTLELDISIFICPEGTRNFT
                     NQLLLPFKDGAFRLAIATQTPLAVLTVINTDVLLSPESPLALRPGKLIAEWSKPIDTK
                     GMTENDVDSLKQQTANLMKETLTRHLMSAQK"
     gene            complement(12962..15094)
                     /locus_tag="PKY09_06375"
     CDS             complement(12962..15094)
                     /locus_tag="PKY09_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNB12519.1"
                     /translation="MRTYDEKHIKNIALVGSAKSGKTTLAETMLFEAGIITKRGSVEE
                     KNTVSDYHEIEHERQSSVYEATMHTEWRDYKINIIDTPGIDDFIGEVISAVRVCDTAV
                     MLLNAQHGVEASSEVIWDYVDKYRKPTIFAVNQLDLEHANFNATVEKAKQKFGNAITV
                     MQYPLNQGTGFNAIIDLLKMTMYRFPATGGKPEKLEIPPEEMENAKKLHNELVEKAAE
                     NDEKLMELYFERGSLDEDEMKQGLKAGMMAHQIFPVFCLSAKKDMGSGRLMGFIDNVA
                     PSAIDMPAEIAEDKTLVPCIPTGPTVLFFYKTIIEPHLGKLAFFKVLSGEVSAGTDLM
                     NHKTNTSERLTQLFIMDGKSRNTVDKLKAGDLGCTLKLKNTFTNHTLCGKGIAINIEP
                     IQFPEPKTRVALIAKNKSDEEKLGEVLAELHLEDPTLVVEYSKELKQVILHAQGDLHL
                     AVTKWRIENVYKMQVEFIKPRISFRETIQKPAIATYRHKKQSGGAGQFGEVHIKIEPY
                     YDGMPDMKEYPLRDKEEHALPWGGKLVFNNCITGGAIDTRFLPSVLKGVMEKMQEGPL
                     TGSFVRDIRVSVFDGKMHPVDSNDISFKIAGMMAFREAFHNAQPQLLEPIYDVEVEMP
                     DDMMGDVMSDLQSRRSIIMGMDSRNGRQVIRAKTPLLELDGYSTALKSITQGKAKLKT
                     SFAEFAPVPGDLQKKLHDEYVKVEALSS"
     gene            complement(15280..19353)
                     /locus_tag="PKY09_06380"
     CDS             complement(15280..19353)
                     /locus_tag="PKY09_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12520.1"
                     /translation="MRITAPCNEPTAQTDLQINNVRARIFSAGDMWWNLVDGANSKAE
                     YEIPKGTGKTSLFAGSVWIGGYDATGNLKVAGQTYRQSDANDFFTGPIGVQGGNMTTD
                     VSICNQYDRIWSVTKQQVRDFVSTGVATRDIREWPGNGDQSRGELQLLAPFEDVNGDG
                     LYIPDSGDYPRYLLSGEYPAPSDPNKLPCDDYIFGDQTLWWVFNDIGNIKKETNSAAI
                     GLEFRAQAFAFSTDDEINNMTFYKYQIINRSNQSFDSTYFGWWVDPDLGDAFDDYVGC
                     DVNLGLGFCYNGDPDDGSGTIDGYGINPPAVGADFFQGPLADANDGVDNDRDGVVDEP
                     GEQIIMSQFGYYENDFSVYGNPQVFDDYYQYLTGSWKNAIRWTRDGANGRTPPSPSTP
                     ITEYMFPGTTDPAFPGNNWTEATANNTPFDRRFIQSAGKFTLQPGAVNYITTGMVWAR
                     ANNGGPLASVDLLKLSDNKAQGLFDVCFKTLDGPDAPDVQITELNNKVILSLKNFNNE
                     RIEKYSQLDPSIPTLVQPDEEQRKFKFQGYLIFQLKNASINTSDIFDPSKTTSSSVMS
                     GDNYRLISQVDIKDTVTNLVNYFWAPELPGYRPLTVAFNPDQLTGTNSGLKHSFVIDK
                     DAFTNKPLVNHKAYYYVVVSYAYNNFKPFEYIVPNSSNFTQQKPFKVGRNNIKVMSAI
                     PHAPEVEHSGLVFNSEVGDGLKITRLEGSGNGGLLIDMTDASAAEALNAPYVVSNPVY
                     ELGKGPLNVTVYDPLYTTTGYFDMKFTGVSGTDKWSIYETVTQSVDTATFELQQPNSQ
                     IIQTVDPNDLSKVYEWGLTADISNAVIEAGNTDAINNGLLDQSIAFANPDAAWLGGVA
                     DRDSSVADARDWIRSGTAPNDIGGADPNQVYEGLIGGTWAPYKLANKTDAQYGPRSSA
                     HQDNQLTMSGPTGITSVDVVFTSDKSKWTRAVVLEMGQPTSATIGNAPRFQKRRSASI
                     NKDGGNNPGGAVSTDPNDPNYIDNEGMGWFPGYAINIETGERLNIAYGENSVFTTENS
                     QDMQWNPTSTMLDGNGNPVFGGMHYIWVFGSRVTQGARDIGKYDACEKFNTLIKATSA
                     AERRDALANIMYVSIPLLNPGRSLLETDAKVRIRVARQYRTFNTNFDVASYINHTGTG
                     SPLTRGQTYAVVSGTVTAAFNNNSITYSPGQTITIPTDANTFPDASITSAGGVVLAPT
                     INGTNPYYRLDEEGMSPTFDNTEVAKGALKLVNVVPNPYYAYSSYDKSTNMMWVKITN
                     LPAKCTVSIYTMNGILVKRFNRNSGSTIDGTAIGLSQGTEAGSGNSDTSVDWDLTNEK
                     NVPISSGVYLIHVKAPGLGERTLKWFGVMRPISLDSY"
     gene            complement(19856..20647)
                     /locus_tag="PKY09_06385"
     CDS             complement(19856..20647)
                     /locus_tag="PKY09_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNB12521.1"
                     /translation="MNKIKSLSLFSAIALAALSGCKKDDLTSDFKTSDPIQNGTAKIR
                     FIHVAPELGGVSVKYNDNSVFGDSLRYSQNSGYTEVALGSVDNGFNLDMIPVEDSTGI
                     FTPNETGVIATLSQSNYILTKDQRYTCFIVDTVKDKPVLNDLSYDGFTKIITENYPAT
                     GTDSVWVRLFNASYSSAIVDVEFTNNTTGAVTTISNVSFMQMPVYAGAGSGDFNVAVK
                     KFGVDLATMSNVNLQSGKAYTFYFRGVIGASGDSEPRLDYLTNGE"
     gene            complement(20713..21108)
                     /locus_tag="PKY09_06390"
     CDS             complement(20713..21108)
                     /locus_tag="PKY09_06390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNB12522.1"
                     /translation="MATIFTKIINGEIPAYKIDENDKYLAFLDINPIAMGHVLVIPKR
                     EVDYIFNLEDEELAGLMAFSKQISKALIQAVPCKRIGVTVIGLEVPHTHIHLVPINNV
                     DDMNFGKPKLKLTNEQLELIRLKIKSFLK"
     gene            complement(21111..21581)
                     /gene="greA"
                     /locus_tag="PKY09_06395"
     CDS             complement(21111..21581)
                     /gene="greA"
                     /locus_tag="PKY09_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNB12523.1"
                     /translation="MSITYLTEEGLKKLQDELTHLKTVQRPLISKQIAEARDKGDLSE
                     NAEYDAAKDAQGLLELKISKMEELLSSARIVDESKIDTSKVSILSKVKIKNLANKQEV
                     TYTLVAENEADLKAGKISVTSPIGKGLLGKKKGEIAEITVPSGKLKFEIVSIDR"
     gene            complement(21658..22710)
                     /locus_tag="PKY09_06400"
     CDS             complement(21658..22710)
                     /locus_tag="PKY09_06400"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNB12524.1"
                     /translation="MTYKFKISAIAITSLLLSASAFAGNEDRVGQSGANELIINPWVR
                     SAGWMNCNTSSVRGLEAQYLNVAGTAFAKKTEIGFTHTNWLKGTGISINSFGITQHVG
                     ESGALTLGIMSMDFGNIEITTPESPEGGIGTYKPQFLNVGLSYAKAFSNSIYGGLTIK
                     IISESVADVRASGIAFDAGIQYVTGFNKEKDNLRFGIALKNVGAPLNFKGDGLASKGF
                     TSDQTSLTISQRAERFELPSLVNIGAAYDFKFLEMHRITLSSTFTSNSFTRDQTAVGL
                     EYGFKKLFAIRAAYSFERKGTSEDESLNVYSGFGAGITVEAPIGKSGKSLGLDYSYRP
                     TYTFDGTHAFGVIFKL"
     gene            complement(22740..26936)
                     /locus_tag="PKY09_06405"
     CDS             complement(22740..26936)
                     /locus_tag="PKY09_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12525.1"
                     /translation="MINKSCLTVFLVSAITVGSVTAKENIGQKPVHKGNVVKNTSLRI
                     TAPCNEPTAQTDLQINNVRARIFSAGDMWWNLVDGANSKAEYEIPKGTGKTSLFAGSV
                     WIGGYDATGNLKVAGQTYRQSDANDFFTGPIGVQGGNMTTDVSICNQYDRIWSVTKQQ
                     VRDFVSTGVATRDIREWPGNGDQSRGELQLLAPFEDVNGDGLYIPDSGDYPRYLLSGE
                     YPAPSDPNKLPCDDYIFGDQTLWWVFNDIGNIKKETNSAAIGLEFRAQAFAFSTDDEI
                     NNMTFYKYQIINRSNQSFDSTYFGWWVDPDLGDAFDDYVGCDVNLGLGFCYNGDPDDG
                     SGTIDGYGINPPAVGADFFQGPLADANDGVDNDRDGVVDEPGEQIIMSQFGYYENDFS
                     VYGNPQVFDDYYQYLTGSWKNAIRWTRDGANGRTPPSPSTPITEYMFPGTTDPAFPGN
                     NWTEATANNTPFDRRFIQSAGKFTLQPGAVNYITTGMVWARANNGGPLASVDLLKLSD
                     NKAQGLFDVCFKTLDGPDAPDVQITELNNKVILSLKNFNNERIEKYSQLDPSIPTLVQ
                     PDEEQRKFKFQGYLIFQLKNASINTSDIFDPSKTTSSSVMSGDNYRLISQVDIKDTVT
                     NLVNYFWAPELPGYRPLTVAFNPDQLTGTNSGLKHSFVIDKDAFTNKPLVNHKAYYYV
                     VVSYAYNNFKPFEYIVPNSSNFTQQKPFKVGRNNIKVMSAIPHAPEVEHSGLVFNSEV
                     GDGLKITRLEGSGNGGLLIDMTDASAAEALNAPYVVSNPVYELGKGPLNVTVYDPLYT
                     TTGYFDMKFTGVSGTDKWSIYETVTQSVDTATFELQQPNSQIIQTVDPNDLSKVYEWG
                     LTADISNAVIEAGNTDAINNGLLDQSIAFANPDAAWLGGVADRDSSVADARDWIRSGT
                     APNDIGGADPNQVYEGLIGGTWAPYKLANKTDAQYGPRSSAHQDNQLTMSGPTGITSV
                     DVVFTSDKSKWTRAVVLEMGQPTSATIGNAPRFQKRRSASINKDGGNNPGGAVSTDPN
                     DPNYIDNEGMGWFPGYAINIETGERLNIAYGENSVFTTENSQDMQWNPTSTMLDGNGN
                     PVFGGMHYIWVFGSRVTQGARDIGKYDACEKFNTLIKATSAAERRDALANIMYVSIPL
                     LNPGRSLLETDAKVRIRVARQYRTFNTNFDVASYINHTGTGSPLTRGQTYAVVSGTVT
                     AAFNNNSITYSPGQTITIPTDANTFPDASITSAGGVVLAPTINGTNPYYRLDEEGMSP
                     TFDNTEVAKGALKLVNVVPNPYYAYSSYDKSTNMMWVKITNLPAKCTVSIYTMNGILV
                     KRFNRNSGSTIDGTAIGLSQGTEAGSGNSDTSVDWDLTNEKNVPISSGVYLIHVKAPG
                     LGERTLKWFGVMRPISLDSY"
     gene            complement(26975..>27871)
                     /locus_tag="PKY09_06410"
     CDS             complement(26975..>27871)
                     /locus_tag="PKY09_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12526.1"
                     /translation="NYELGFRQILSRSSVITISAFYRELKQMLQLRNIFFSYPGSGYT
                     TFDNIDFGTVKGLTLGYDLRRTGNVRLGASYTLQFADGTGSNDVSGADLLSTGQAGLR
                     VILPLDFDQRHNITVSFDYRYQEGKNYNGPVLFGKQIFANTGARLEFKAGSGTPYSRQ
                     YTPTFEGNNIGVQTQGNSKIKGDINSARLPWNSRFDLKVDKDFALNFDKDKKTKYYLN
                     VYVQVQNLLNQKNIISVYRYTGNADDDGYMGSSLGQDFLSNLEASGGDPQAFSDLYAV
                     KINNPDNYARPRTVRVGLSFNF"
BASE COUNT         8346 a         5814 c         4871 g         8840 t
ORIGIN      
        1 taccatcagt ggccctgtag taactgttga ctctgtaaaa aatgcgcgtt gctttagtca
       61 ggctaatgga gcagtttata tttctgttac aggaaataat ggccctgtta cctatttgtg
      121 gagtaatgct gctaccactc aggacattgt taacattgct gcaggaactt ataccgttac
      181 tgttactgat tcaacaggtt gtgttgggaa taaaactcaa ataatcacac aaccttcagt
      241 attaacatta attgacacaa tagtaccagc gcgatgtgga aatgcaaatg gtagtgcagg
      301 agttaatgtc acaggtggaa cttcgccata cacctatttg tggagtaatg gcgcttctac
      361 ttcatcaata tcgggattaa acggaggcac ctacacagtt actgtaaccg atagcaaact
      421 atgtactgca aatcgtacac ttgttgttaa caatacaccg tccttagtta tttctattga
      481 cactattata catgtaaaat gtgcaggaca aaacacagga gttattaaag tcaatgtaac
      541 cggtggatca ggagcattaa cctatacatg gacaccatca caaataggca ccgattcaat
      601 tttcaatctg gcttccggaa cctataaatt agttgtcaga gatactgcca actgcaggga
      661 ctcaataagc gtaacaatta acagcccgac agcaattaat gctacaataa actcattgcc
      721 tgaaacatgc ggaattaata atggtcaggc ctgggtgact gtttcaggcg gaacacctgg
      781 atatactttc ctgtggaata acagttcaac gaatgacacc attacgaatt taaccaaggg
      841 aaactattct gtcacaatca aggatgcaaa caactgtagt gtcattaaaa acatattggt
      901 tgattcactt ggtgggcctg caattacaga ctctataatt catgtaaaat gttttgggga
      961 aaacaccggt gctatttata caacagttca tggcgctaat ggtcctgtta gttatttatg
     1021 gtctccgggt ggtcaaatta cacaaaatat aaccaatctg actgcaggta catatactgt
     1081 tacagttaca gacacgaaca gctgttcttc aacaaaaagt tttgtagtta atcaaccaac
     1141 aggtttaaat atttctttta cttcagttac tgaaaaatgt aatcaggcga acggttctgc
     1201 cattgtcgca gtaactggcg gaacttctcc atactcctat caatggaaca atggcaatac
     1261 tacaaataac atcatcaatt taacatcagg atattacacc attacgatta ctgacgggaa
     1321 tctgtgtaca aaaagagatt ctgtttttat cgggcataca aatggtccat caatatcaaa
     1381 cttcagtcat aacttaatca aatgtcataa tgatagtagt ggctcactga ctgttaatgt
     1441 tacaggtggt acaccatttt acaactatca atggagtgga ttccccttag ttaacaatgc
     1501 aacacttcag aatattcctg caggagtata tacagtaact gtgactgatg ctaatggctg
     1561 tacagtaaat gcaacagaca gtcttcagaa tcctccctta atgtcaattt cgcatacact
     1621 taccaatgca tcttgtaatt taaataatgg tattgctcag ataaacgttt ctggtggtgc
     1681 ttctccctac atctacaact ggagtaatgg tgttacaggt gcaactcttt tggatacttt
     1741 ggcaaatagt ttttacacta ttaccataac ggatgcttta ggctgtataa agacagatac
     1801 atttactatt cagcgtactc ctgctacctt tatccaactt acatcattaa attatgttac
     1861 ttgctttgga ttaagtaatg gaagtattac catcacacca tctggtggtg atggtaatta
     1921 tacttaccaa tggtcttaca acaatattca aactcagaac ctgaataaca ttcctgcagg
     1981 ctcctatgat ataaccgtaa ctgatggaac aggttgtagt tacacaatgc cggatattca
     2041 ggtttcccag ccggatactt taattgcaca agttacaaca tcaccaacag ggtgcacgtc
     2101 acctacaggc agtgcaacgg caaatcaaac cggaggtata cagccttaca cctatctatg
     2161 gtcaaccaat gcaacatctc aaacaatcaa tggcctgtca agtcaaatta ttacagtaac
     2221 cataacagac aataatggat gtacagcaac caattccgga ataatcagtg cattatcagc
     2281 tcctgttgtc aatatttcag gcacacaaaa catcaattgt tttggaggta caaatggatt
     2341 tattaataca actgtaacgg gaggaaatca gccatacaat tacctctgga ataacggttc
     2401 aaccacatca aatatcaata acctttcagc aggcacttac acactaacgg taacggactc
     2461 tgcgcagtgc agtacaatta ttacacaaac tctgacagaa aacccaaaaa ttcaatttgg
     2521 tttatcttta attgcaggcc catgtaacgg aggaactccg gcaattgcca tttcagcacc
     2581 ttcaggcgga gttgcgccat taaatctttt gtggtcaaat ggcagcactt ccacatctgc
     2641 acaagctact gctggaactg taatgacttt aactattact gacaatgcag gatgcagcag
     2701 agatacaaca ttcagcatgc tttcaaccaa cggcccacaa atttcaagta ttcaaacaac
     2761 agacatcgtt tgttttggtg atagcagcgg cagtgcaaca gtaaacattt caggaggaac
     2821 acccccaata aatattcaat ggattggcac tttaccggtt caaaccacag caactgcaat
     2881 aaatttaaaa gccggaagct atacatgcat tgttacagac gtaaatggct gttcagcaca
     2941 aagtgttgca aatattaatc acctgaattc attaatcaca ccaacactca caataactaa
     3001 tgctagttgt ggaaacagca acggtcaaat tgttgtaaat gcaactggtg gtggcggtac
     3061 ctatcagtac aaacttaata atggagttta ttcttcaaca aacacatttg ctaacctctc
     3121 cgcaggatct gactcggttt ttgttaaaga caacaacgga tgtgaagttt ttgttatttc
     3181 aaccatcagt aatatacagg gacctgtaat atcagttata gactcggcta acgtgagttg
     3241 ctttggcagt aatgatggtt tcattatttt aaatgtaacc ggtgcagcac agcccatcac
     3301 ctattcatgg tctgatggac caacaacctt aacaagaaat aatcttgggc ccggtaacta
     3361 tacatttact gcacaggatg ccaacaattg tatatctgta agaaatttta tcattacaga
     3421 accgacaaca attacaatca gtgaaataat tccaaacaaa aatggtgttt acaatattac
     3481 ttgtaacaca ctagctgacg gagaaattca ggtaatagca aatggtggtg ttccgtttac
     3541 tcaaacatct gcttacaact atcaatggtc aataagtggc gcttccggag catctgtaac
     3601 aaatcttact gcaggcccat acactgtaac agtcaccgac tctatggggt gctctgccac
     3661 aaaatcattc ctcctgactg agccacctgc cttaaatgca ggtgctgatt tcacacaatc
     3721 tatttgtgga actgacacca ttgtacttat aggcaatatc ccatcgtatg gatttggaaa
     3781 atggactgtt cgcagtggaa gtggaattat tgctgacccg gattctatca caacgcagat
     3841 tactgcaatt gcaaatggag taaatgtttt tgcttgggtg gtttatgaca atttctgttc
     3901 agacactgca ttatatgtca tcaacaaaaa agaaaaaatt gtagctgatg gcggtattga
     3961 tcaaaatgtg tgtgacaatt ttgttttcct ttcagcagta ccaccacctt caggtaccgg
     4021 ggtatggcaa agcataaatg gaaatagcac aatagaaaac ccatcatcac cagcaacgaa
     4081 ggttgaaaat ttaagtccgg gtaaaaatat ttttgtctgg acaattacca atggcacatg
     4141 tactgcatct gacacagtaa agattgctat tcttcctccg gaagaatgtt ttactgatgt
     4201 tgttatgcca acaggtttta ctcccaatga agatgggtat aacgacttat atgttatcag
     4261 aggattagat aatacaaaca atactttgac aattttcaac agatggggca acttagttta
     4321 tgaaaagaaa aactataata atgactggga tggaaagtct aataataaac aaatattacc
     4381 tgacggaact tactatgcca ttttacggat acctgccagg aatttagtct taaaaacata
     4441 tattgattta agaagataag cttatgaaaa aattatgttc cataataatt atgttcattt
     4501 tgtctgacta tttgtttgca cagcaggctc caatgacaac acaatacatg tttaatacac
     4561 tgctgataaa tccggcttat gcgggaagta aaccattcac aagtgcaaca atgatggtaa
     4621 gaaaacaatg ggcaggattt aaaggatcac ctacaacaga aactgcaagt atccatggtg
     4681 cattggaaga taaaaatgtt ggcttaggat tatacataag taatgatcat gcaggtatta
     4741 taaatcgcac agaactcttc ggaagttatt cttatactat tgaatatgga gagtcaaaac
     4801 ttgcattcgg tcttcaggca ggtttaagtt atttgaaatc tacgctatca gatctggttt
     4861 ataacgacaa caatgacccg gtttacgaaa agaacaccat aaacaacgta cttcctaatt
     4921 ttggagctgg tttatattat catactgatt tattttatgc aggtatttct gctccgctgc
     4981 ttttaagtta cgacccgctt aaatcactgt ctcttgaaat gaccgatgta catcatatca
     5041 gaagacacta ttttctgacc acaggttatg ttttttcatt aagtgagcag gttaaattaa
     5101 aaccatcagt tcttctaaaa tatgtagatg gtgcaccatt acaattcgac attaatgcaa
     5161 atctgctttt gaacaatatc atttggcttg gagcttcata ccgctccaat gatgcaattg
     5221 cagtcattgg tgaatatcag ctatctaaaa aattgcgaat tggttattca tttgactatt
     5281 cgcttaccct actcagaaac cattcagcag gatcgcatga atttatgctt ggatacgatt
     5341 ttggttataa tgtacctaaa ataaaaactc cgcgttattt ttaattatga atagaaaacg
     5401 caacattttt ttattgtttg ttattttatt tttcacatcc tgtttatctg tcaaacaaag
     5461 aacagctgac acctattatg agcagaactt atatgacaaa gctattccgt attatcagga
     5521 agttactaac aaaaaattta atttagatat ccttattaaa cttgcagatt gttatcgtaa
     5581 tgtaaagaac tatgcactat ctgaacagat ttattctaag atagtaacat ctaataccga
     5641 cccgatttat cagttttatt acgctgaagc actaatgcaa aatggcaaat atgttgaggc
     5701 aagaaaatgg ttaaacaaat ttctagttct aaatcgtgcc gatagcaggg cattaaacct
     5761 attagcatct tgcgattcaa tagaaatgct ttataaagac tctttactct ttgatgtcag
     5821 acgactacca tttaacggaa ttgatgagag taatttctcc cctgaattat tccattccgg
     5881 aattgtttat gttagcaaca gaaacactga tccaaaaaat tataaaacga atcctgttat
     5941 tgataattac aacttatttt atgcacaaca aactgaatta gggcattgga tggacccggt
     6001 tccattacga ggcacaatta attctatttt taatgaaggt ccatgcacat tcaataatga
     6061 attcacaaaa gcttacttta cacgcaatgc taatgaagga aaaaaaattg tcacgaatca
     6121 gcaaagtgaa aatgttttag aaatttttga agctacaacc aaggatggtg aatggaatgt
     6181 ttccggacct gtaagtttca acaacaacaa ttacagtgta ggccatccat ctatttcacc
     6241 aagcggtaat actatggttt ttgtttcaga tatgccatgg ggctatggcg gtactgatat
     6301 ttatagagta caatggataa ataatacatg gagtaaaccg gctagtatcg ggaaggttat
     6361 taatacaagt ggaaatgaaa tgtttccgtt cttactcaac gactcaatac tttacttttc
     6421 aagtaatggt atgattggtg ttggaggtct tgatatttat cgtacagagt taactgctga
     6481 tggatggaca gtacctgaaa acttaggcta ccccattaat agtcccagag acgattttgg
     6541 tttgatttgt gataatgaag aattaagcgg ctatttcagc agcaacaggc aacaaggcaa
     6601 agacaatatt ttttcattta aaaggaatgc tccggtttta acgctaaaag gcaaattaat
     6661 tgacaagtca acaaacaagc ctttgaaaaa tattaatctt aagttgaccg caaattcaat
     6721 tgatactatt ttaacaacag atggcgaagg taactttaca gcacaattaa aaaacaataa
     6781 agactacaca cttcgcgctg aagacaaaag ttattttaca acttcagaga atatttctac
     6841 gtttggttac agaaaatcag caacactgaa taaggattta actttagaaa aagtgatttt
     6901 caacaagcct gttatttgga gaggcattgc atttgaaaaa ggaaaatcag atttaacacc
     6961 gtcaactaaa acagagttaa ataaactgtt caatatatta cttgagaata aaaacataaa
     7021 aatagaatta agctgtcata cagactcacg aggtgtagaa cgtgataacc tgatattgtc
     7081 acaagccaga gcagataaag cggcagccta tcttatcaat aaagggattt caaaagacag
     7141 aattatttca gtgggatttg gttcacaacg cttattaaat aattgtgtaa ggggtatatt
     7201 atgtttagaa gaagaccatc aaattaatat aagaactgaa ataagataca gcccttatca
     7261 aagataagac ttaacagtca gaatacattt ttctgtaaag atggccaacg tattaggcat
     7321 ttccgcattt tatcatgact cagcagcgac tattgccatt gatggtaaaa tagtagccgc
     7381 agttcaggaa gaacgttttt caagaattaa aaatgatgca aaatttcctg taaacgcaat
     7441 aaaatattgt ttgcaatatt ccggtttaaa gttaaatcaa attgatcata ttgtatatta
     7501 tgaaaaaccg tttctcaagt tagagcggtt attggaaacc tatctagaaa ccgctcctaa
     7561 tggttttcag caatttactc aggctgtgcc actttggatt aaagaaaaag ttttgataaa
     7621 aaataaaatc agagaaaact taaatttgat tgatgagaat ttttcggcaa aagaaatact
     7681 attttgcgag catcatcttt cgcatgcagc aagtgcatta tatacctctc ccttttccaa
     7741 tgcagcaata ataactgctg atggtgttgg cgaatggtgt actacctcta tcatgaatgg
     7801 tacaccaaat actattacct ctttaaaaga actcaacttt ccacactcaa ttggtttgtt
     7861 atatagtgca tttacacagt tcctggggtt taaagtaaat tcaggcgagt acaaagtaat
     7921 gggcttggct ccttatggaa ataagaatag ttcgcaattt agttttttta aaaataaaat
     7981 cagtgaacta tttgacctga agtcagacgg gtctgttttt ctgaatatac gatactttaa
     8041 ttttcaaaaa gggttaagca tgataaatga aaatctttgg gctgagcttt ttgaaataaa
     8101 accacgtatt gctgaatctg aaataaattt aaatcattgc catttagcat tagccattca
     8161 aacgattaca gaagatgtca tttttaattt agctaaacag gccaaacaaa ttacaaatgc
     8221 agacaattta tgtttggcag gaggtgtagc attaaattgt gttgccgttg gtaaactcaa
     8281 caagcaaaat attttcaata aaatttatat tcaacctgcc tccggtgatg caggtggtgc
     8341 tattggcgca gcacttgcaa ttgagcatat atttttaggt aacaaacgta aaactcctga
     8401 aaaagatcat ttgcaaggat gtcgcttagg tcctgcttat gacaaccaaa ctgttgcaca
     8461 gtctttaaca aaatacaatt tacattttga acagtttaaa gaagaagagt tactaaaact
     8521 cactgccaca catctatcaa ataataaaac tgtagcgtgg tttcagggaa gaatggaatt
     8581 tgggccacgc gctcttggat caaggagtat tcttgctaac ccctcaacat cgaacattca
     8641 acaaacatta aatcaaaaaa taaaaaaacg tgagtcattc agaccatttg cgcccgccat
     8701 gctaattgaa gattatttag aattttatga aggaggcaat gaaaatggtt tcatgtctat
     8761 gacagatttt cttaaaaaag aaaaaagaat gggtaatgac tcaactcctg aagaagctgg
     8821 tataacagaa caattacaaa aatctaaaag tgctattcaa ggagtaacac acgttgacta
     8881 tagctcacga attcaggtgg tgactgaaac tgatgacagc ttatttttta aactactaac
     8941 agaatataaa aagataaccg ggagaggaat actcattaat accagtttca atctaagagg
     9001 acaaccaata gtttgcaatc ctgatgatgc ctgtaagtgt tttctggaaa cagaaataga
     9061 cttattggca ataaataatt ttattgtcag aaaaaaataa ctaaagcaaa ttcacacagt
     9121 gattactcta cttttacagc ttcgcctgat ttaattttct cctgaatcca ttgtgttgtt
     9181 acagaacttg gtctttcaat aggcattcct aatgcacgat cccataaaag tgaagtcaac
     9241 acgccaatag ctcttgaaac accaaataaa actgtataaa aatcatattc tactaagcca
     9301 taatgcatca acaatatacc tgagtgagca tcaacgtttg gccatggatt tttaatctta
     9361 cctgtagctt ttaatatttc cggtgtaaca tcatacagca attgaactat tttaaacagt
     9421 tcatcatctg ccatatattt ttttgcaaaa tcctgttgcg cagtataacg gggatcggtt
     9481 ttacgcagta cggcatgtcc gtatccggga acaactttac cttctgttaa tgttgtttta
     9541 ataaactcac ctaattgttc gcgggatgga acgccattat atttatcgcg caactcaatt
     9601 atccatcggg ctacttcctg tgccgctaat ccatgtagcg ggcctgcaag tccgttcata
     9661 cctgccgcca atgaaagata aggatcactc aatgctgacc caacaagatg tgtggcatgt
     9721 gctgaaacgt ttcctccctc atgatcggca tgaatggtaa gatataatcg catcaacttt
     9781 ctgaaatcaa gttcttcaaa tcctagcatg tgtgcaaaat tccctgccca gtcaagcttt
     9841 ggatccggct caatgtgttt gccttcctta taagtagaac ggtaaatgta tgctgcaata
     9901 cgaggaagac gtgcaatgag gttcatgaca tcttcgtaca taggatccca ataatccttc
     9961 ttattcattc cttcacgata cttacgtgca aaaacagact cagtctgcaa agccattatt
    10021 gccattgaaa attgtgtcat cggatgtgtc ttttcaggca acatatccaa catatcaaaa
    10081 acatgtttag gaacggttgc tcttttcatc cattcattat taacttcaat aacgtctttc
    10141 agtaacggca tttcaccggt gagcaagagg taaaaaatcc cttcaggcaa tggctccggg
    10201 ccttcttttg atacaggaag tttttcacga agttcaggaa tagaatatcc tctgaaacga
    10261 attccttcat tggcatcaag cttactggtt tctgttacca tgcctatcat tcctttcatt
    10321 ccctgataga cctgttctac tgtatattga cccagtacaa aattaccgtg tgtttttaca
    10381 atatctttaa ttgtacctgc catcggcaag gctttctctt tgaatttttc ttttatgttg
    10441 tccatacttg ttgtataact gcttcttaaa cgtttgttac aaattaaagt ttacagagaa
    10501 gtcctttgtt gaaggaatta tctctactct tgatttaggt ttcttggtat tttcatagcg
    10561 aaggtcagac tctgaaaaca gcacaattaa agttgtaata aaaatggcaa caacgataat
    10621 ccatgcaaaa atgcgcaaat actttttaat cttcttcttt ggatcaggtt tctttttaat
    10681 tcttatacgt ttacgaatcc tgattctttc tttctttatt tcaccatctg attcttccaa
    10741 ttccattttc aagttgttaa ttcagattct aaagatacag cacctccggc attaaatcaa
    10801 attacggaag ttttttccgg gaaattcagc agcaccaccc agcatttctt caatacgcag
    10861 gagttggttg tacttagcaa tcctgtcaga acgagatgca gaaccggttt tgatctgtcc
    10921 tgtatttagt gccactgcca aatcagcaat agttgtgtct tccgtttcac ctgaacgatg
    10981 actcatcact gatgtgtagc tgttgttctt agccatttca acagcattga tagtttccgt
    11041 caaagttcca atctgattta cttttaccaa aattgagttg gcaatacttt ctttaattcc
    11101 tcttgcaagc ctttctgtat ttgtcacaaa caaatcatca ccaactaact gcactttacc
    11161 tcctaacaca ttggtcaatt ctttccatcc tgcccaatcg tcttctgcaa gtccatcttc
    11221 aatactaaca atcggatact tctcacacca ctgcttccaa tagctaacca tttctgatga
    11281 tgtcagtttt tctccacttg attttttaaa atgatatacc ttattctctg catcataaaa
    11341 ttcagaagca gcagcatcca ttgcaatcat gacatcatca cctggtttgt aacctgcttt
    11401 ttctattgct tctatcactg tcaatatagc ttcttcgtta gatttgatgt ccggtgcaaa
    11461 accaccttca tcaccaacgt ttgtagaata cttcttgctt tttaaaacac tcttcagatt
    11521 gtgaaatatt tcaacaccca ttcttagtga ctcagaaaaa ctttctgcac caaccggcat
    11581 tatcataaat tcctgaaagt ctatactatt atctgcatga gatcctccat taaggatatt
    11641 catcataggt acaggcaata tgcgagcatt cacaccaccg atataacggt aaagcgggta
    11701 gcccgattcg cttgcagctg ctctggcaca tgccaatgaa acacctaata ttgcattggc
    11761 acctattgta gctttattag gagaaccatc aatatgaatc attgctgcat caatactgcg
    11821 ctgatcaaaa acaggataac catgaagttc tttatttaat atggcattta cattattcac
    11881 agctctcatc acactcttgc caaggtaaat tttcttatcg ttatcacgta actcaacggc
    11941 ttcatgtgct ccggttgatg cacctgatgg cacggcagct cttcctacaa ctccattatc
    12001 tgtatataca tcaacttcaa tagtaggatt tcctcttgag tcgagtattt gcctggctct
    12061 gatttcagca atatttccca taatatttta ctctttataa aacggctgca aagttaacgt
    12121 aatgtgccgt aaatttattt aatttagcga aataaatttc aaccatacac ataacactaa
    12181 tagcgctata tgaaggtgct taaaattgca tttggattat atgcagcaat agtgtttttt
    12241 atcactttgc tttgtgtttt ccctctatat tttatagtat ttttattttt cagtgaaagg
    12301 aaatctcctg ttatagcaca taacatttca cgattttggg cattggtact gttcagaagt
    12361 ttttttatcc gatacaaaat tatcaatgaa gactttattt cagataaagg aacctatgta
    12421 tttgttgcca accatcgttc tatgttggat attccactct atgctcgagc atgcagcaat
    12481 actttcagat tcttatctaa ggcagagttg actaaaattc cgttgctagg ctatgtcatc
    12541 cgaaaactct acgtcactgt aaaacgtggt gacaaaaaag acagacaaaa aagtatagag
    12601 gcgatgcgca aaacactgga gttggacatt tcaatattta tttgccctga aggcactcga
    12661 aattttacaa atcagctatt gctgccattt aaagatggtg catttcgttt agctattgca
    12721 acacaaacac cattggcagt acttacagtg ataaatacag atgtactttt atcacctgaa
    12781 tcaccattag cattgcgtcc gggcaaactg attgcagaat ggagtaaacc tattgacacc
    12841 aaaggaatga ctgaaaatga tgttgattca ctaaaacaac agacggcaaa cctgatgaag
    12901 gaaacgctga cacgtcattt aatgtcagct caaaaataat tttcgttttt gttgaattac
    12961 ttcaactgct tagagcttct actttcacgt actcatcatg caatttcttt tgcaagtctc
    13021 cgggcacagg agcaaattca gcaaaggaag tttttagctt agcctttcct tgtgtgatag
    13081 attttaatgc tgtactataa ccatcaagtt caagcaatgg agttttagca cgaatcactt
    13141 gtcttccatt tcgtgagtcc attcccatta ttatacttct tcttgactga agatcactca
    13201 ttacatcacc catcatatca tcaggcattt cgacttcaac atcataaata ggctctaaaa
    13261 gttgtggctg tgcattgtga aatgcttcac gaaatgccat catacctgct atcttaaatg
    13321 atatatcatt tgaatcaaca ggatgcattt taccatcaaa cacacttact ctaatatcgc
    13381 gtacaaacga gcctgttaat ggaccttcct gcattttttc cattacacct ttaagtacag
    13441 aaggtaaaaa gcgtgtatca atagcacctc cggtgataca gttattaaaa accaattttc
    13501 caccccaagg cagtgcatgc tcttctttat cacgaagtgg atactctttc atatctggca
    13561 tgccatcata gtaaggttca attttaatat gtacttcacc aaattgtccg gccccacccg
    13621 attgcttttt atgtctgtat gttgcaattg caggtttttg aatagtttca cggaatgata
    13681 tgcgtggttt aataaattca acctgcattt tatatacatt ctcaatccgc cattttgtta
    13741 cagcaagatg caaatcgcct tgtgcatgta aaataacctg cttgagttct ttcgaatact
    13801 caactactaa ggtaggatct tcgagatgca attcagcaag tacctcaccc aatttctctt
    13861 catcactttt atttttagca atcaatgcca ctctggtttt tggctcagga aattgtattg
    13921 gctctatatt gattgcaatg cctttaccac aaagcgtatg atttgtaaat gtattcttca
    13981 acttaagagt gcagcctaaa tcacctgctt taagtttatc aacggtattg cggcttttgc
    14041 catccatgat aaacaattgt gttaatcttt cgcttgtatt tgttttgtga ttcattaagt
    14101 cagttcctgc actgacctca ccggaaagaa ctttaaaaaa cgccagctta cccaagtgcg
    14161 gctcaatgat agttttataa aagaacaaaa ctgttggtcc tgtcggaatg caaggaacta
    14221 aagttttatc ttcagcaatt tctgccggca tatcaattgc cgaaggtgcc acattatcaa
    14281 taaaacccat tagtcgcccg ctacccatat ctttctttgc agataaacaa aacacaggga
    14341 aaatctgatg tgccatcatg cctgctttta atccttgctt catttcatct tcgtcaagtg
    14401 atccgcgctc aaaatataat tccatcagtt tttcatcatt ctcggcagct ttttctacca
    14461 attcattatg caactttttg gcattttcca tttcctccgg tggaatttca agtttttcag
    14521 gcttgccacc agttgcagga aaacgataca tcgtcatttt caataaatca ataatcgcat
    14581 taaaacctgt tccctgattt aacggatact gcataacggt aatggcattg ccgaacttct
    14641 gttttgcctt ttctaccgtt gcattaaaat tggcatgctc aaggtcaagt tggttgacag
    14701 caaatattgt tggcttccgg tatttgtcca cataatccca tatgacttca ctgcttgctt
    14761 caacaccatg ttgtgcattt agcaacataa cagccgtatc acatactctc actgcagata
    14821 ttacttctcc tataaaatca tctattcccg gtgtatcaat aatattaatt ttataatcac
    14881 gccattcggt gtgcatagtc gcttcataaa cagaactctg tcgttcatgt tcaatttcat
    14941 gataatctga aaccgtattt ttttcttcaa cagaacctcg cttggtgata atacctgctt
    15001 caaagagcat ggtctctgcc aatgttgttt taccactttt tgctgatccg acaagcgcaa
    15061 tattctttat gtgtttttcg tcataagttc tcatacaacc tttgtttaaa atgaggtatg
    15121 aaaattatgg aacttaaagt aaacttgcaa ataaatcaac cacctgtcat caaaatttaa
    15181 tgagaagtcg tcactttaga gataaatcgc caataacaag ttgctgacac agttgtcatg
    15241 gttagatttt attaaaacat taaaggccag caagtgtaat taataactat ctaaactaat
    15301 cggacgcatt acaccaaacc acttaagtgt tcgctcacct aagcccggtg cttttacatg
    15361 tatcagatat acaccactgc tgataggtac attcttctca ttcgtcaagt cccagtcaac
    15421 agaggtgtcg gaattaccgc tacctgcctc tgtgccctga ctcaaaccta tcgctgtgcc
    15481 atctatcgtt gatcctgaat tgcggttgaa gcgctttacc aggattccat tcattgtgta
    15541 tattgacact gtacattttg caggcaggtt tgtaatcttt acccacatca tgttcgttga
    15601 cttatcatac gatgaatatg cataatatgg attgggtacc acattcacca gcttcagtgc
    15661 acccttggct acttctgtat tatcaaatgt aggtgacatg ccttcttcat ccaatctgta
    15721 atatgggttc gtaccattta ttgttggtgc cagaactact cctcctgctg aggtgatcga
    15781 ggcatcaggg aaggtattag catcggtcgg tatggttatc gtctgtcccg gtgaatacgt
    15841 gatgctgttg ttgttaaacg ctgccgttac tgttccactc actactgcat aggtctgacc
    15901 tcgtgtcaat ggagatcctg tgcctgtatg attgatataa gatgctacat cgaaattggt
    15961 attaaacgtc ctgtactgac gtgctactcg gattcttacc ttggcatctg tctccaataa
    16021 acttctgccc ggattcaaca agggtatgct aacatacatg atgttcgcta atgcatctct
    16081 tctctctgct gctgaagttg cctttatcaa cgtgttgaac ttttcacatg catcgtactt
    16141 gcctatatct cttgcaccct gtgttactct tgatccaaat acccagatat aatgcatacc
    16201 gccaaacacc ggattgccat tgccatccaa cattgttgat gttggattcc actgcatatc
    16261 ctgactgttt tctgtggtaa acacagagtt ctcaccatag gctatgttca agcgttcgcc
    16321 tgtctctata tttattgcat agcccgggaa ccaacccatg ccttcattgt ctatatagtt
    16381 cggatcatta ggatctgttg atactgctcc cccgggattg ttaccgccat ctttgtttat
    16441 tgacgctgat ctgcgtttct ggaaacgtgg tgcattacct atcgttgcac tggtaggctg
    16501 tcccatttcc aacaccacag ctcttgtcca ttttgactta tcactcgtaa acactacgtc
    16561 aacactggtg atgcctgttg ggccactcat ggttaactga ttgtcctgat gtgctgatga
    16621 gcgtgggccg tattgtgcat ctgtcttgtt ggctaattta tatggtgccc aggtgccgcc
    16681 tatcaaccct tcataaacct gattcggatc cgctccgcct atatcattag gcgctgtgcc
    16741 actgcgtatc caatctctgg catctgcaac acttgaatct ctgtccgcta caccgccaag
    16801 ccaggctgca tcagggttgg caaacgctat actctgatcc aacaatccat tattgattgc
    16861 atccgtattg cctgcttcta ttactgcatt gctgatatct gccgtcaagc cccactcata
    16921 taccttactt aaatcgttgg gatctaccgt ttgtattatc tggctgttgg gttgctgcaa
    16981 ttcaaatgtt gctgtatcta ccgattgggt tactgtttca tatatactcc acttatcggt
    17041 tccgctaact cccgtaaact tcatgtcaaa atatcccgtt gttgtatata atggatcata
    17101 caccgttaca ttcaacggac ctttgcccaa ttcatatact ggattggata ctacataagg
    17161 ggcattcaac gcttctgctg ctgaggcatc tgtcatgtct atgagcaaac ctccattgcc
    17221 tgaaccttcc aatcgtgtta tctttaatcc atctcctact tccgagttaa acactaatcc
    17281 actatgctct acttccggtg catgaggtat ggccgacatt accttaatgt tgtttctgcc
    17341 taccttaaag ggtttctgct gggtgaagtt ggaggagttg ggtactatgt attcaaatgg
    17401 tttgaagttg ttgtatgcat agcttaccac tacataatag tatgccttat gatttactaa
    17461 tggcttgttg gtaaaggcat ctttatctat tacaaaactg tgtttcaatc cactgtttgt
    17521 tcctgttaac tgatccggat taaatgccac tgtcaatggg cgatagcccg gcagttcagg
    17581 tgcccagaaa tagttgacta agtttgttac tgtatctttt atgtcaacct gacttatcag
    17641 acggtaatta tcaccactca ttacacttga tgatgtggtc ttggaaggat caaatatatc
    17701 tgaagtattt atactggcat tctttaactg gaatatcaga tagccctgaa acttaaactt
    17761 acgctgctct tcatcaggtt gtaccaatgt tgggattgat ggatctaact gtgagtattt
    17821 ttctatcctc tcattattga agttctttaa tgaaagaata actttattat ttaactctgt
    17881 tatctgtaca tcaggtgcat cagggccgtc caatgttttg aaacatacat caaacaagcc
    17941 ttgtgctttg ttgtcgctaa gctttaacaa atctactgat gccaatggac ctccgttgtt
    18001 agcacgtgcc cataccatac ctgtagtgat gtagtttacg gctccgggtt gtaaggtgaa
    18061 ctttccagct gactggataa atcttctgtc gaatggagtg ttgttcgctg ttgcctctgt
    18121 ccaattattg cctgggaatg caggatcggt tgtgcccggg aacatatact cagtgatcgg
    18181 ggttgaaggt gaaggagggg tgcgaccatt agcaccatca cgagtccatc tgattgcatt
    18241 tttccatgaa ccagtcaagt attgatagta atcgtcaaaa acctgagggt ttccataaac
    18301 actaaaatca ttttcgtaat aaccaaactg cgacatgata atttgttctc caggttcatc
    18361 cactactcca tctctgtcgt tgtcaacacc gtcattagca tcagctaacg gtccttgaaa
    18421 aaagtctgca cccactgctg gtggatttat tccatatccg tctatggttc cactgccatc
    18481 atcagggtcg ccattataac aaaagcctaa tccaagattt acatcacagc ctacataatc
    18541 atcaaaggca tcacccaaat cagggtcaac ccaccagcca aaataagtac tgtcgaatga
    18601 ctgattgctg cggttaatga tctgatactt ataaaatgtc atgttgttga tctcatcgtc
    18661 cgtgctgaag gcaaaggcct gtgctctgaa ctcaagacct atggctgcac tgttggtctc
    18721 ttttttaatg tttcctatat cattaaatac ccaccataat gtctgatctc caaaaatata
    18781 atcatcacat ggcaatttat tcggatcgct tggtgcagga tattcaccac tcaataaata
    18841 tcgtggataa tcgccactgt ctggtatata caaaccatct ccgtttacat cttcaaacgg
    18901 tgccagtagc tgcaactctc ctcttgactg atcgccattt ccaggccact ctctgatgtc
    18961 gcgtgtagcc actcctgtag atacaaagtc gcgcacctgt tgtttggtta ccgaccatat
    19021 tctgtcgtac tgattacaga tggaaacatc tgttgtcata tttcctccct gcacacctat
    19081 tgggccggta aaaaaatcat tcgcatcact ttgtctgtac gtctgacctg ccacttttaa
    19141 gttcccggta gcatcgtaac cacctatcca tacagagcct gcaaacaacg atgtttttcc
    19201 tgtaccctta ggtatttcat actctgcttt tgagttagcg ccatctacaa gattccacca
    19261 catatcgcct gcagaaaata ttcttgcacg aacattgtta atctgcaaat ctgtttgtgc
    19321 tgtaggctca ttacaggggg ctgtaattct caaagacgta ttctttacaa cattgccttt
    19381 atgtaccggc ttctgaccta tattctcctt tgctgtgact gaaccaactg ttattactga
    19441 taccaggaaa accgttaaac aactcttatc aataattagt gttttgtttt agttatggtt
    19501 aataatcact ttaataattc aaagtaatac ttgttaaata agcctttata ttctaacaca
    19561 tcatgattgc cgatttcgtt tttaattttg ataaaaaaat atttttaata atttaaaatt
    19621 attgtattgg gtgattttgc aataataatt tcatgtgttc ttaaactatt atatcatcat
    19681 ttatttgcgg tattaaaagt atgcaatttt ataataatta caaatagcta aaagtaaaaa
    19741 tctgtggaaa ttaaattcag gcttattcaa tattgtaatt gaataaaggc agaatcaagg
    19801 gcaaaaaaaa ggctgccttg ttctcaaagc agccttttaa gtataatgaa taaatttatt
    19861 ctccgttagt taaataatca agacgtggtt cagaatcacc tgaagcgcca atcacaccac
    19921 ggaagtaaaa tgtataagct ttgcctgatt gcaggtttac attactcata gttgccaaat
    19981 caacaccgaa tttctttact gcaacattaa aatcaccgct accggcacct gcataaacag
    20041 gcatctgcat aaagctaaca tttgaaatgg ttgtaactgc accggttgtg ttatttgtaa
    20101 attcaacatc aactattgct gaagaatatg atgcattaaa caaacgcacc caaacagaat
    20161 cagttccggt tgcaggatag ttttctgtga tgatttttgt aaagccatca taagaaagat
    20221 cattaagtac aggtttatct ttaactgtat caacaataaa acaagtgtat cgttgatctt
    20281 tagttaatat ataattgctt tgtgacagag ttgcaattac acctgtttca ttaggtgtga
    20341 aaattcctgt tgaatcttct acaggaatca tatccaaatt aaatccattg tcaactgagc
    20401 ctaaggcaac ttcagtataa ccggagtttt gtgaatagcg taatgagtcg ccaaacacac
    20461 tattatcatt atatttgaca gaaacaccac ctaattcagg tgctacgtga atgaaacgaa
    20521 tttttgccgt accattctga attgggtcag aggttttaaa atctgatgta agatcatctt
    20581 tcttacaccc tgaaagtgct gccaaggcaa ttgctgaaaa caatgaaagg ctttttattt
    20641 tattcataat ttaaaaatat acaggttaaa atgcagatac gaaataacgt aatttaaatt
    20701 aaaactgaaa atttatttta aaaacgattt aattttcagc cggattaact ccaactgctc
    20761 attagtcaac tttaatttgg gcttgccaaa gttcatatcg tcaacattat tgatgggcac
    20821 cagatgtatg tgtgtatgtg gcacttcaag accaataact gtaacaccaa ttcttttgca
    20881 tggcacagcc tgtattaatg ccttgctgat ttgcttggaa aatgccataa gacctgccaa
    20941 ctcctcatct tctaaattaa aaatataatc aacttcacgc tttggaatta ccaaaacatg
    21001 ccccattgca ataggattaa tatccaaaaa agctaaatat ttatcgtttt catcaatttt
    21061 ataggctgga atttcgccat taataatttt tgtgaaaatg gttgccatga ttatctatct
    21121 attgaaacaa tttcaaactt aagctttccg gaaggaactg taatttcagc aatctcgcct
    21181 ttctttttac ccaaaaggcc ttttccaatc ggagatgtta cagaaatctt tcctgctttc
    21241 aaatcagcct cattttccgc aaccaaagta taggtcacct cctgcttatt agctaaattt
    21301 ttaatcttga ctttcgacaa aatagacact tttgatgtat ctattttcga ctcatcaaca
    21361 atacgtgcac tgctcaacaa ttcttccatt ttactgattt tcaattccag taatccttgc
    21421 gcgtctttag ccgcatcgta ctctgcattt tctgataaat cacctttatc tcttgcctca
    21481 gctatttgtt ttgaaataag gggtcgctga actgtcttca agtgtgtaag ttcatcctga
    21541 agcttcttta atccttcttc tgttaaatac gtaatagaca tcttcttgtg ggtattaaaa
    21601 aaatgaaaga aggtctgtta agacctcctt taaaataatt taaaaattaa atagttatta
    21661 caatttgaaa atcacaccaa aggcatgcgt accatcaaat gtataagttg ggcgataaga
    21721 ataatccaat ccaagtgatt ttccactttt acctattggg gcttcaactg ttatacctgc
    21781 accaaatcca ctataaacat tcaatgattc atcttcactt gttccttttc tttcgaatga
    21841 ataagcagca cgaatagcaa acagtttttt aaatccatac tccaaaccaa ctgctgtctg
    21901 atcgcgtgtg aaagaattag atgtaaatgt tgatgacagc gttatacgat gcatttctaa
    21961 aaacttaaag tcataagcgg caccgatatt tactaatgaa ggaagttcaa accgctctgc
    22021 acgttgtgaa atggttaacg atgtctggtc agaagtgaat ccttttgatg ctaatccatc
    22081 acctttaaag tttaatggtg ctccaacatt tttcaaagca ataccaaagc gaaggttatc
    22141 cttttctttg ttaaaccctg ttacatattg gattcctgca tcaaaagcaa ttcctgaagc
    22201 acgtacatca gcaacacttt ctgaaataat ttttatagtt aaaccaccat aaatactatt
    22261 cgaaaatgct tttgcataag ataatcctac attaagaaat tgaggtttat aagttccaat
    22321 tccaccttct ggtgattctg gagtggtgat ttcaatatta ccgaaatcca tagacatgat
    22381 acctaaagtt aatgctccgg attctcccac atgttgtgta ataccaaatg aattaatgct
    22441 gatacctgtt ccttttagcc aattggtatg tgtaaaacca atttctgtct tttttgcaaa
    22501 agcagttccg gccacattca ggtattgagc ttccaatcca cgaactgaag aagtgttaca
    22561 gttcatccag cctgcactgc gaacccatgg gttaataatt aactcattgg cacccgattg
    22621 tccaacacga tcttcattac cggcaaatgc tgatgcagat agcaataacg atgtgattgc
    22681 tatagctgat attttgaatt tatatgtcat atttcaatat attttggtta ggaggtttat
    22741 taataactat ctaaactaat cggacgcatt acaccaaacc acttaagtgt tcgctcacct
    22801 aagcccggtg cttttacatg tatcagatat acaccactgc tgataggtac attcttctca
    22861 ttcgtcaagt cccagtcaac agaggtgtcg gaattaccgc tacctgcctc tgtgccctga
    22921 ctcaaaccta tcgctgtgcc atctatcgtt gatcctgaat tgcggttgaa gcgctttacc
    22981 aggattccat tcattgtgta tattgacact gtacattttg caggcaggtt tgtaatcttt
    23041 acccacatca tgttcgttga cttatcatac gatgaatatg cataatatgg attgggtacc
    23101 acattcacca gcttcagtgc acccttggct acttctgtat tatcaaatgt aggtgacatg
    23161 ccttcttcat ccaatctgta atatgggttc gtaccattta ttgttggtgc cagaactact
    23221 cctcctgctg aggtgatcga ggcatcaggg aaggtattag catcggtcgg tatggttatc
    23281 gtctgtcccg gtgaatacgt gatgctgttg ttgttaaacg ctgccgttac tgttccactc
    23341 actactgcat aggtctgacc tcgtgtcaat ggagatcctg tgcctgtatg attgatataa
    23401 gatgctacat cgaaattggt attaaacgtc ctgtactgac gtgctactcg gattcttacc
    23461 ttggcatctg tctccaataa acttctgccc ggattcaaca agggtatgct aacatacatg
    23521 atgttcgcta atgcatctct tctctctgct gctgaagttg cctttatcaa cgtgttgaac
    23581 ttttcacatg catcgtactt gcctatatct cttgcaccct gtgttactct tgatccaaat
    23641 acccagatat aatgcatacc gccaaacacc ggattgccat tgccatccaa cattgttgat
    23701 gttggattcc actgcatatc ctgactgttt tctgtggtaa acacagagtt ctcaccatag
    23761 gctatgttca agcgttcgcc tgtctctata tttattgcat agcccgggaa ccaacccatg
    23821 ccttcattgt ctatatagtt cggatcatta ggatctgttg atactgctcc cccgggattg
    23881 ttaccgccat ctttgtttat tgacgctgat ctgcgtttct ggaaacgtgg tgcattacct
    23941 atcgttgcac tggtaggctg tcccatttcc aacaccacag ctcttgtcca ttttgactta
    24001 tcactcgtaa acactacgtc aacactggtg atgcctgttg ggccactcat ggttaactga
    24061 ttgtcctgat gtgctgatga gcgtgggccg tattgtgcat ctgtcttgtt ggctaattta
    24121 tatggtgccc aggtgccgcc tatcaaccct tcataaacct gattcggatc cgctccgcct
    24181 atatcattag gcgctgtgcc actgcgtatc caatctctgg catctgcaac acttgaatct
    24241 ctgtccgcta caccgccaag ccaggctgca tcagggttgg caaacgctat actctgatcc
    24301 aacaatccat tattgattgc atccgtattg cctgcttcta ttactgcatt gctgatatct
    24361 gccgtcaagc cccactcata taccttactt aaatcgttgg gatctaccgt ttgtattatc
    24421 tggctgttgg gttgctgcaa ttcaaatgtt gctgtatcta ccgattgggt tactgtttca
    24481 tatatactcc acttatcggt tccgctaact cccgtaaact tcatgtcaaa atatcccgtt
    24541 gttgtatata atggatcata caccgttaca ttcaacggac ctttgcccaa ttcatatact
    24601 ggattggata ctacataagg ggcattcaac gcttctgctg ctgaggcatc tgtcatgtct
    24661 atgagcaaac ctccattgcc tgaaccttcc aatcgtgtta tctttaatcc atctcctact
    24721 tccgagttaa acactaatcc actatgctct acttccggtg catgaggtat ggccgacatt
    24781 accttaatgt tgtttctgcc taccttaaag ggtttctgct gggtgaagtt ggaggagttg
    24841 ggtactatgt attcaaatgg tttgaagttg ttgtatgcat agcttaccac tacataatag
    24901 tatgccttat gatttactaa tggcttgttg gtaaaggcat ctttatctat tacaaaactg
    24961 tgtttcaatc cactgtttgt tcctgttaac tgatccggat taaatgccac tgtcaatggg
    25021 cgatagcccg gcagttcagg tgcccagaaa tagttgacta agtttgttac tgtatctttt
    25081 atgtcaacct gacttatcag acggtaatta tcaccactca ttacacttga tgatgtggtc
    25141 ttggaaggat caaatatatc tgaagtattt atactggcat tctttaactg gaatatcaga
    25201 tagccctgaa acttaaactt acgctgctct tcatcaggtt gtaccaatgt tgggattgat
    25261 ggatctaact gtgagtattt ttctatcctc tcattattga agttctttaa tgaaagaata
    25321 actttattat ttaactctgt tatctgtaca tcaggtgcat cagggccgtc caatgttttg
    25381 aaacatacat caaacaagcc ttgtgctttg ttgtcgctaa gctttaacaa atctactgat
    25441 gccaatggac ctccgttgtt agcacgtgcc cataccatac ctgtagtgat gtagtttacg
    25501 gctccgggtt gtaaggtgaa ctttccagct gactggataa atcttctgtc gaatggagtg
    25561 ttgttcgctg ttgcctctgt ccaattattg cctgggaatg caggatcggt tgtgcccggg
    25621 aacatatact cagtgatcgg ggttgaaggt gaaggagggg tgcgaccatt agcaccatca
    25681 cgagtccatc tgattgcatt tttccatgaa ccagtcaagt attgatagta atcgtcaaaa
    25741 acctgagggt ttccataaac actaaaatca ttttcgtaat aaccaaactg cgacatgata
    25801 atttgttctc caggttcatc cactactcca tctctgtcgt tgtcaacacc gtcattagca
    25861 tcagctaacg gtccttgaaa aaagtctgca cccactgctg gtggatttat tccatatccg
    25921 tctatggttc cactgccatc atcagggtcg ccattataac aaaagcctaa tccaagattt
    25981 acatcacagc ctacataatc atcaaaggca tcacccaaat cagggtcaac ccaccagcca
    26041 aaataagtac tgtcgaatga ctgattgctg cggttaatga tctgatactt ataaaatgtc
    26101 atgttgttga tctcatcgtc cgtgctgaag gcaaaggcct gtgctctgaa ctcaagacct
    26161 atggctgcac tgttggtctc ttttttaatg tttcctatat cattaaatac ccaccataat
    26221 gtctgatctc caaaaatata atcatcacat ggcaatttat tcggatcgct tggtgcagga
    26281 tattcaccac tcaataaata tcgtggataa tcgccactgt ctggtatata caaaccatct
    26341 ccgtttacat cttcaaacgg tgccagtagc tgcaactctc ctcttgactg atcgccattt
    26401 ccaggccact ctctgatgtc gcgtgtagcc actcctgtag atacaaagtc gcgcacctgt
    26461 tgtttggtta ccgaccatat tctgtcgtac tgattacaga tggaaacatc tgttgtcata
    26521 tttcctccct gcacacctat tgggccggta aaaaaatcat tcgcatcact ttgtctgtac
    26581 gtctgacctg ccacttttaa gttcccggta gcatcgtaac cacctatcca tacagagcct
    26641 gcaaacaacg atgtttttcc tgtaccctta ggtatttcat actctgcttt tgagttagcg
    26701 ccatctacaa gattccacca catatcgcct gcagaaaata ttcttgcacg aacattgtta
    26761 atctgcaaat ctgtttgtgc tgtaggctca ttacaggggg ctgtaattct caaagacgta
    26821 ttctttacaa cattgccttt atgtaccggc ttctgaccta tattctcctt tgctgtgact
    26881 gaaccaactg ttattgctga taccagaaaa accgttaaac aactcttatt aatcatttgt
    26941 gctttgtttt taaatatttt aattaagtct gagattaaaa gttaaatgat aaacctacac
    27001 gaactgttct tggacgtgca taattatccg gattgttgat ttttaccgca tataaatcag
    27061 agaatgcctg tggatcacca ccggaagctt caagattact tagaaaatcc tgacctaagc
    27121 tggagcccat ataaccatca tcatcagcat ttccggtata acgataaaca ctaattatat
    27181 ttttctgatt taaaaggttt tgcacctgaa catatacatt taagtagtat ttagtctttt
    27241 tatctttatc aaaattcaaa gcaaaatcct tatcaacctt caagtcaaaa cggctattcc
    27301 atggaaggcg tgcagagttg atatctccct tgattttaga attaccttgt gtttgaacac
    27361 caatattatt tccttcaaaa gttggtgtat attgacgtga ataaggagta cctgatccgg
    27421 ctttaaactc taagcgtgct cctgtgtttg caaaaatttg tttaccaaac aatacaggac
    27481 cattataatt tttgccttcc tgataacgat agtcaaaaga tactgtgatg ttatgtctct
    27541 gatcaaaatc aagtggtaga attactctca aacctgcttg tccggtactt aacaaatcag
    27601 ctcctgaaac atcatttgag cctgtaccgt cagcaaactg aagtgtatag cttgcaccaa
    27661 gacgaacatt acctgttctt ctaaggtcgt aacctaaagt taagccttta acagtaccaa
    27721 agtcaatatt atcgaaagtt gtgtaaccgg aaccgggata agaaaagaaa atatttctca
    27781 attgaagcat ttgctttagt tcacgataga atgcagaaat tgtaattaca gaactacggc
    27841 tcaatatctg acggaaaccc aattcataat t
//
LOCUS       DAOQNP010000035        27269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_573_length_27269_cov_7.490703, whole genome shotgun sequence.
ACCESSION   DAOQNP010000035 DAOQNP010000000
VERSION     DAOQNP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 27269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27269
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_573_length_27269_cov_7.490703"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(432..776)
                     /gene="rplT"
                     /locus_tag="PKY09_06415"
     CDS             complement(432..776)
                     /gene="rplT"
                     /locus_tag="PKY09_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNB12527.1"
                     /translation="MPRSVNSVASRARRKKLLNRAKGNWGARGNVLTVAKHTVEKGLQ
                     YAYRDRKTKKRNFRGLWIQRINAEVRNYDMSYSEFMGKLHKAGNQLNRKVLADLALNN
                     PDAFKAIVESVK"
     gene            complement(860..1057)
                     /gene="rpmI"
                     /locus_tag="PKY09_06420"
     CDS             complement(860..1057)
                     /gene="rpmI"
                     /locus_tag="PKY09_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNB12528.1"
                     /translation="MPKMKRNSSAKKRFSVTGTGKIKRKHAFKSHILTKKSTKRKRAL
                     TNAAIVHKSDEFNIKRMLSIA"
     gene            complement(1092..1589)
                     /gene="infC"
                     /locus_tag="PKY09_06425"
     CDS             complement(1092..1589)
                     /gene="infC"
                     /locus_tag="PKY09_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNB12529.1"
                     /translation="MNGRIHSPQVRLVGENVEPRIYDTQTALNMAQDMGLDLVEISNT
                     SNPPVCKIVDYQKFLYERKKKDKEQKAKTAKSVVKEIRFGPNTDEHDFNFKLKHAIKF
                     LEEGAKVKAYVHFKGRSIAYKEKGEIILLKFAQELEQYAKVEMLPKLEGNRMFLHLAP
                     LAKKK"
     gene            complement(1689..3626)
                     /gene="thrS"
                     /locus_tag="PKY09_06430"
     CDS             complement(1689..3626)
                     /gene="thrS"
                     /locus_tag="PKY09_06430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNB12530.1"
                     /translation="MKITFPDKNVREYPDGTTAMDIAKSISEGLARNVLAAKINGQVS
                     DLSRPINNDASLELLTWDSQDGKNTMWHSSAHLMAEALEFYFPGIKFWVGPPVENGFY
                     YDVDLGGKQISSDDFKKIEDKMIELARKNNVYIRKEVPKQEAIAYFTEKNDEYKLDLL
                     QNLEDGNITFYTQGNFTDLCRGPHIPHTGYIKAVKLMNLAGAYWKGDEKNKQLTRIYG
                     ITFPKQKELDEYLAMLEEAKKRDHRKLGKELDIFMFDDEVGPGLPLWLPNGGVLIEQL
                     ENLAKRTENEAGYKRVRTPHLAKESMYLTTGHLPYYAESMFPPMEMDGEKYYLKPMNC
                     PHHHKIFGNLNASYRDLPIRLAEYGTCYRYEQSGELFGLMRVRCLQMNDAHIYCTEEQ
                     FASEFKAVNDMYLKYFKIFGIEKYIMRFSTHSPEKLGKKYVNEPELWLKTEDMVRRVL
                     IESNIPYIEVPDESAFYGPKIDVQVWSAIGREFTLATNQVDFAVPARFGLTYRDADNT
                     MKTPLCIHRAPLGTHERFIGFLIEHYAGNFPLWLAPMQVAILPISEKFNDFAKSVSQL
                     LNNYDIRALVDERGEKIGRKIRDAEVKKVPFMLIVGEKEMNEKQVSVRKHGEGDKGSM
                     TPEAFCQLVKKEIEDQLLINK"
     gene            3717..5243
                     /gene="guaA"
                     /locus_tag="PKY09_06435"
     CDS             3717..5243
                     /gene="guaA"
                     /locus_tag="PKY09_06435"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNB12531.1"
                     /translation="MHQTILIIDFGSQYTQLIARRLRELNVYCEIHPYNHLPEMSDSI
                     KGVIFSGSPFSVRDSLAPFIDTKSFFGKTPVLGLCYGAQLLAHQHQGEVVASKIREYG
                     RAILKINFDDEIFNDVSDNSQVWMSHADTIRSAGNDFEVIAQTDDVAVAAFRHKHLAV
                     YGFQYHPEVYHSTDGKKMLANFVYNICGCRGDWTPSSFVDETVAQLQEKLKNDKVILG
                     LSGGVDSSVAALLLHKAIGKNLYCIFIDNGLLRKNEAHHVMEVFKPLGLNIKCVDAAS
                     VFYEALKNISDPEQKRKTIGRVFIEVFDKEAHTISDVKWLAQGTIYPDVIESVSVKGP
                     SATIKSHHNVGGLPEKMNLKIVEPLRSLFKDEVRKVGKEMGLKEELLNRHPFPGPGLG
                     IRILGDISVEKVRMLQEADHVFISLLHKHNLYQKVWQAGAIFLPVNSVGVMGDERTYE
                     NAICIRAVTSTDGMTADWAHLPYEFLSIVSNEIINKVKGINRVVYDISSKPPATIEWE
                     "
     gene            5200..6459
                     /locus_tag="PKY09_06440"
     CDS             5200..6459
                     /locus_tag="PKY09_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB12532.1"
                     /translation="MTSVQSHLQQLNGNRTMDLRSNRDYLIWVLVFALLSGCSSSRKT
                     SQMPPPAPAPKPAEQPVKKPEVVHEKPEKKEFRIGLMLPLNLEQQIQMFDDTSGNAIP
                     DYNNLSNLQFVEGAQLAADSLKQLGINVNLNIAETPADSFALWRLWRQNNFVKCDAVI
                     SSVPSSFIPAVTRLSANSKQTLILTQPSNTNFKSTNEHVFMLSPSTYTQCMVAAKEIA
                     TRFPDFKYVLLARDVKPDKDIAQIFKDHLPAKHLKQFISSSFIPDSVCKILKSEKAVL
                     IITSSAEAYVNTILNQVNACIEKPALAIGLPTWENFESIDFSALKNLRIAFFTSTYLD
                     YHAASTKSFRNSFINHYKTEPFISAFQGYEAVMEYVKSNTGHGSLKSGYLPTLNIKYA
                     TDTANGIKENQGINLIEIKDYTLIPLE"
     gene            6608..6841
                     /gene="rpmB"
                     /locus_tag="PKY09_06445"
     CDS             6608..6841
                     /gene="rpmB"
                     /locus_tag="PKY09_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNB12533.1"
                     /translation="MSRICDLTGKTSIVGNNISFSNRKTKRRFNPNLQTKKFFIPETG
                     EWVTLKVSASAIRTINKKGISACINSLIKKGHI"
     gene            6854..7036
                     /gene="rpmG"
                     /locus_tag="PKY09_06450"
     CDS             6854..7036
                     /gene="rpmG"
                     /locus_tag="PKY09_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNB12534.1"
                     /translation="MAKKGNRVQVIMECTEHKNSGMPGTSRYITTKNKKNTTERLELK
                     KYNPILKKVTLHKEIK"
     gene            7054..7206
                     /locus_tag="PKY09_06455"
     CDS             7054..7206
                     /locus_tag="PKY09_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HNB12535.1"
                     /translation="MAKKVVATLKTGKGKEFSKVIKMVKGANGAYSFKEEIVHNDHVK
                     DFLKEK"
     gene            7290..8240
                     /gene="ftsY"
                     /locus_tag="PKY09_06460"
     CDS             7290..8240
                     /gene="ftsY"
                     /locus_tag="PKY09_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNB12536.1"
                     /translation="MGFFGLFSKKDKESLDSGLNKTRENFFTKITKAVIGKSTVDDEV
                     LDNVEEILVSSDVGVDTTLKIVKRIQDRVARDKYVGTDQLNTILKEEIAALLAENKSA
                     TVANLIDESKKPYIIMVVGVNGVGKTTTIGKLAYQFKQSGKNVLLGAADTFRAAAVDQ
                     LNIWAERVQVPIVSQGMNADPASVAFDTVKSGVAQNSDVVIIDTAGRLHNKVNLMNEL
                     TKIKRVMQKVVPDAPHEILLVLDASTGQNAIEQARQFTAATEVNALALTKLDGTAKGG
                     VVIGISDEFKIPVKYIGVGEKITDLQAFDRVSFVDSLFAK"
     gene            complement(8249..9379)
                     /locus_tag="PKY09_06465"
     CDS             complement(8249..9379)
                     /locus_tag="PKY09_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12537.1"
                     /translation="MRTLRLIGCGLLISIIASCSNGNSSGQETDEETETSTTSQATDK
                     NSSSKNSNWEKLVAVENRDANGNLVSTIPLPSNWKIHNIRQKGDPTFTGPDGIVVKDY
                     PIQMFNYPFDQNTQYLYSQSGQQVRDFPGVEQVIQQDLIPVCRNQGLSFIKYYELPDV
                     SRIEKWYSDQLYQAVPNDKKTFAIGTDWQTADGNPYFLIVHFSISTTEQLRMWSYFVS
                     SLEADKDVFEKAKKQLLFSISNVRYELEPIMAYNRSEAEKAGRSWAQFNARMAQNQAN
                     FEASQRAHINKTNAINDAIMSGWRERNAASDRMQEQRIDGIYERTNVVDESGQKYKVE
                     SGYNQYWMNNDGEYISTDKVDYNPNLDDNMNDQKWQELKEIK"
     gene            complement(9521..10489)
                     /locus_tag="PKY09_06470"
     CDS             complement(9521..10489)
                     /locus_tag="PKY09_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNB12538.1"
                     /translation="MNQHYTSDVEAIKDLVNSYQKLRTEIGKVIVGQDAVVRDVLISI
                     FSKGHCLLVGVPGLAKTLLVNTISQALGLSFNRIQFTPDLMPSDITGIEILDENRHFK
                     FIKGPVFANIILADEINRTPPKTQAALLEAMQERSVTVSGKRYDLPIPFFVLATQNPI
                     EQEGTYPLPEAQLDRFMFNVFLDYPSFEEEVQVVKNTTGDKIGKVEKVLEENEITFFQ
                     ELIRKIPVADNVLNYAVGLTAKTRPGAANKSVMINDYVAWGAGPRASQFLVIGAKCHA
                     AINGKYSPDIEDVKAVAVPILRHRLVRNYKAEADGVSVEKIIDSIL"
     gene            complement(10507..11826)
                     /locus_tag="PKY09_06475"
     CDS             complement(10507..11826)
                     /locus_tag="PKY09_06475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB12539.1"
                     /translation="MVFIMPSLLYAQKSDTVIDQIIGIVGNKIILQSDLEAQYQQYLA
                     QGHYKDESAKCDVLDQLLLTKLLIHYANIDSVTVSDAQVEGEIEKRIAYFTEQFNGST
                     EKLEEYYQKSVPEIKDEFRPLVKEQLLAQTMQQKIIGKASASPADVKAYFNSIPKDSL
                     PYINSELEYGEIAFKIAVSDEEKNKVKTRLEEIRTRLLKGEDFATLAILYSQDAGSAK
                     NGGELGFVPRGQFVPEFEAIAYKLKKGEISPIIETKFGYHVVQLIDRRGETINVRHIL
                     LKPEVSNKDLLATKAKADSIAFDIRSGKIKFEDAAIQFSDAEDSKNNGGNVVNPATGN
                     TRFEANQVDAMVFFQLDKLKEGEVSNPALITSRDGSQYYKIFLLKKRTQPHVANLTDD
                     YQRIQQAALSHKQAKLMDDWIVRKRKSTYVRIDPEYSHCEEIKKWSN"
     gene            complement(11843..12730)
                     /locus_tag="PKY09_06480"
     CDS             complement(11843..12730)
                     /locus_tag="PKY09_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12540.1"
                     /translation="MKKQHITFNGSGLLLAFAGTVFLMSCNNLPVDFKKDKNKNAIAR
                     VYDKYLYSYEIKDLVPKDATPQDSAMIVKSFVDDWIKQNIVLHQAENNLLDEQKNVEK
                     QLQEYRNSLITFAYEQELVRQRLDTTVTDQEIEQYYNDNSKNFLLKSNIIKATFIKAA
                     KKTPKIDKLKSWFNSSKEKDRQLLEEFCFQYAIDYSLNDNDWMQFDDLLKRVPIKTYD
                     KEEFLRNNRNIETADSANIYLVKITDFKTNENVSPLAFVKDNIKNLIINKRKLALIEQ
                     MQKSAFDQALKNNDFEIYK"
     gene            complement(12742..14751)
                     /locus_tag="PKY09_06485"
     CDS             complement(12742..14751)
                     /locus_tag="PKY09_06485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB12541.1"
                     /translation="MKNFNALGIILISCFFTNAYAQKKTNSATVKPTKAVTAPVAISD
                     PVLVNVAGQNITRSEFERVYYKNNNKGTANDEKSIRDYMELFINYKLKVKEAESLKMD
                     TSAAFKDELTGYRKQLAQPYLTDKDVNDNLVKEAYNRLQNDVRASHILIKLSSDALPK
                     DTLAAYNRIMKIREKIVKGADFEKMARDSSEDPSAKDNGGDLGYFTGLQMVYPFETAA
                     YNTKPGQVSMPVRTRFGYHIIKVSDIRPAVGEIHTAHIMIKVNKSDNDSVQKEAKRKI
                     DEIYTKFKAGEKFEDLAKQYSDDKGSAVNGGVLPWFGTGKMVPEYEKASFALLKDNDV
                     SAPIKSAYGWHIIKRLEKRGIASFDEKKNELKNQIMRDSRNEMGKASLVAKIKKENNF
                     KEFAANKDEYIKSLDTTLADGTYNDSTAIKKTKPLFNLAGKDYTQFDFAAYLAKHQTK
                     RKDITSQAIAHSQYENFVTDACIALEESQLDKKYPDFKALMDEYRDGILLFDLTDKMV
                     WSKAVKDTSGLEQFYEKNKNNYMWGERLDAVIYTCKNADVAKEARKMIKKKKSDSEIA
                     ATLNKDSSLNINIKDGKYSKGDNEIIDKVEWKPGLSADMKDNNQVVFADVKSKLAVQP
                     KKLDEARGLITADYQNFLEKSWITELRKKYSYSVNEDVLKSMFAK"
     gene            complement(14861..15577)
                     /locus_tag="PKY09_06490"
     CDS             complement(14861..15577)
                     /locus_tag="PKY09_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB12542.1"
                     /translation="MAYEFYDATIVDIIDENDVVKRYFIKVPDDRPFSFKAGQFVMLD
                     LPIDSKYTNRSYSIASAPSTDNIFELCIVLNPKGMGTPYMWENYKVGTTVKLSRVLGK
                     FQIPEPIDTDLCFICTGTGIAPLRSQVIDILNRKIPHKNLYMVFGNRWTKDILYRQEM
                     EQLEKDYPQFKFIPVLSRDNEGWTGRKGYVHQVYEEIFADKRPAKFFICGWADMLKEA
                     RQRLEAMGYDKKAIKFESYD"
     gene            complement(15703..16566)
                     /locus_tag="PKY09_06495"
     CDS             complement(15703..16566)
                     /locus_tag="PKY09_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNB12543.1"
                     /translation="MRQLKITKQVTNRETYSLDKYLSEIGKLPLLTSEEEVKYARKIK
                     EGDQHALEMLTKSNLRFVVSVAKQYQNQGLTLGDLINEGNLGLIKAAYRFDETRGFKF
                     ISYAVWWIRQSILQAISEQARIVRLPVNKLGFINKINKTYAQLEQDFEREPSNEEIAN
                     VLHANLDEINDTLRSAGKHISMDAPLLQGEDGNMLDLIPTEDGQGPEVSLMSESLKRE
                     IERALTTLTPREADVIRLYFGLTPEPAMTLEEIGERFELTRERVRQIKEKAIRRLKHT
                     SRSKILKVYLG"
     gene            16859..17926
                     /locus_tag="PKY09_06500"
     CDS             16859..17926
                     /locus_tag="PKY09_06500"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB12544.1"
                     /translation="MKPELEQEGSAQEHLEQDELFEHHRIVVDNGQVQMRIDKFLTAR
                     MANVTRNRLQVAIDSGSILVNDLPTKANYKVKPGDMIRVVLPYPVREIELLCEDIPLN
                     IVYEDKYLAVVNKPAGMVVHPAYGNYTGTLSNAMAGHFYPEFKGQALSKDVFKPGMAH
                     RIDKNTSGLLVVAKDEDTLNYLALQFYERTINRNYIALAWGDIKQQKGEIEGHVGRNK
                     INRKVMDVFPDGSYGKYAKTHFEVIERFGYVTLIKLKLETGRTHQIRVHMQHIGHPLF
                     GDFEYGGDKILKGTTENKYKSFIHNCFELMPYQALHAQSLGFMHPVNGEIKFFEADLP
                     ENFKQLLEKWRNYTSSTHRDG"
     gene            18002..18637
                     /gene="plsY"
                     /locus_tag="PKY09_06505"
     CDS             18002..18637
                     /gene="plsY"
                     /locus_tag="PKY09_06505"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNB12545.1"
                     /translation="MINAENITLLLAAYLLGSIPSAVWIGKVFFNTDVREYGSGNAGA
                     TNTFRVLGPKAGVPVLLMDIAKGWMAVQLATYFGEYMPATQQFINFKLTLGVAALLGH
                     IFPVFAGFRGGKGVATLLGILWGVNPPAAVICMGVFLLFFLTTGYVSLGSMAAAVSFP
                     FIVMWWLNETVTSMNVFAIAVPILVLVTHQKNIERLLKKTESRVYLFRRKQ"
     gene            complement(18643..19848)
                     /gene="pcaF"
                     /locus_tag="PKY09_06510"
     CDS             complement(18643..19848)
                     /gene="pcaF"
                     /locus_tag="PKY09_06510"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HNB12546.1"
                     /translation="MKQAYIVDGIRTPVGNYGGILSSVRPDDMAAHVIKELMARNSGL
                     APEKIEDVILGCANQAGEDNRDVARMALLLAGLPVTVPGETINRLCASGMSAVINASR
                     AIRTGDTSCMIAGGVESMSRAPYVMSKSSSAFARDVQLFDTSIGWRFVNVRMKEMYGT
                     DSMGETAENVAAQYKINREDQDLFALNAQKKAAKSQREGRFECEISPVIIPRKKGDPI
                     VASIDEFIKPDTSIEILSKLKPAFKKEGSVTAGNASGVNDGACAILLTDEDTLTQNKL
                     KPMARIVSSAVAGVEPRIMGIGPVPAANAALKKAGLNWNNIDIIELNEAFAAQALACI
                     RQWNLDDRDERINPNGGAIAIGHPLGMSGARIILTAAHQLTLTKKQYALCTMCIGVGQ
                     GYAVIIERC"
     gene            complement(19853..20275)
                     /locus_tag="PKY09_06515"
     CDS             complement(19853..20275)
                     /locus_tag="PKY09_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNB12547.1"
                     /translation="MDQTTHAQKVVNHMMEHDRFSQWLGIKCLVMEPGKSVLQMQIRE
                     EMLNGFGLIHGGIAFSLADSALAFASNAYGRLSVALECSISYPVPVHNGDIITATTEE
                     LSLTNRIGVYQITISNQNNIKVAFFKGTVYRTSKEYFK"
     gene            complement(20343..21752)
                     /locus_tag="PKY09_06520"
     CDS             complement(20343..21752)
                     /locus_tag="PKY09_06520"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNB12548.1"
                     /translation="MLTDSSAKLNFSEDTVLFDTVFASIGSTTQYLLVHNDHNRPIKI
                     SSIKLAGGSSSPFRMNVDGVAANSLNDIEIRAKDSLWIFIEVTIDPTSDLMPFIVTDS
                     IIFETNGNIQDVDLVAYGRNAHFITPKHAITFADGSGLPYSIIGESSPCGTVIEWDSL
                     LPYVVYGYAVVDSCLTLKINAGTKVYFYNNSGLWVYRYGNLQVEGNLEHPVLFTGSRL
                     ESSYSEVPNQWDRIWINEGGTNKINYAIIKNGLFGVQAEDVQYDGVIGDAPTQLTLSN
                     SIIRNMSIGLLARSFNITAYNNIISDCKSYCTVFSQGGTYNLKQNTIANYWRYSQRST
                     PAFFMNDFVNDGSSNVHIPLNITVDNCIIYGGNDNEIEKDLLDSLADTYSFKNNLIKV
                     DEKITPISVIPSFINTLKNQDPLFEDQSKYNFILKDNSPAIDAGDGSLLTFPELNTDI
                     DGNTRPQGAAPDIGALEKK"
     gene            complement(21904..22527)
                     /locus_tag="PKY09_06525"
     CDS             complement(21904..22527)
                     /locus_tag="PKY09_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB12549.1"
                     /translation="MTLKRIFLLVVTLLSSMTSFAQFAFNPQIGLSVAKLRNLESEES
                     TKARVGWHAGADFRIGRRFYVQPGIYYWQTSTAFINDSLRIQTDLQRSDIKLNARLGL
                     NLLDDENAKIRFSIGPSYNVLISKSAKSDDLDKVTLGELEDNFNNGVFNFDAGLGVDF
                     WKISFDLGYSIGLSDALDNTAYYKSEARYGTFYFNVGLVIGNATSSK"
     gene            complement(22592..23260)
                     /locus_tag="PKY09_06530"
     CDS             complement(22592..23260)
                     /locus_tag="PKY09_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB12550.1"
                     /translation="MTDTIVSLQEANIYVKKNLILTGVNLHVQKNEFVYLIGKTGSGK
                     SSLLRVLFGDIKLNQGEGNVAGYSLNKLKNKEIPFLRRKLGIVFQDFQLLGDRTAKDN
                     LLFVLKATGWNNKQKTDDRINEVLQKVGLGTKGFKMPHELSGGEQQRLAIARALLNEP
                     ELILADEPSGNLDPETSNEIMNLFFELSKTRCSVIMATHNYSLIERFPARIIKLETGK
                     VLES"
     gene            complement(23257..23460)
                     /locus_tag="PKY09_06535"
     CDS             complement(23257..23460)
                     /locus_tag="PKY09_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNB12551.1"
                     /translation="MYLLKIKGTAKIPDYVQMRDDNFTLIAYFRADRPEKAIAKAGLS
                     DKEEYLLKIIAEIPFGKVQKIEL"
     gene            23896..>27269
                     /locus_tag="PKY09_06540"
     CDS             23896..>27269
                     /locus_tag="PKY09_06540"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB12552.1"
                     /translation="MRKNLLNIFLTLLIAAGMHYKSMATHSMGADLTYECVGGNNYKI
                     RVAFYRDCIGINAPNSVNVTISSASCGQRFTRTLFPVPGTGQEITPLCPNALSTCKGG
                     VFTGIQEWVYERVVTLPAQCTDWTFSYELCCRNAAINTITNPLTNTFYIYATLNNTIS
                     PCNTSPVFSNKPVPFACLGQELCFNHGASDADGDSLVYELITPKQNDSTDVSYIPPFS
                     ASNPLTSSPSVYFNTSTGDICMTPTQLDVTVMAVLVKEYRNGVLIGTVERDIQVTVIN
                     CNNTLPTLSGINGTDQFSDTICANQPYCFNVYSNDPDAGQNLTVVYDSSVTGATFTTA
                     GTPHPTGTFCWTPTNSDISGAPRCFTIKVTDDNCPWLGSQIYSYCLTVVGVNVNAGPD
                     QSITCSDLATVTATASGGTGGPYTYQWSNGFTNPTQTVGPGTYIVTVSDGMCSATDTV
                     VIQYISFPTAAFTASSACLNQAITFTDQSTVSGSSIIDWFWDFGDGNTATVQNPIHLY
                     DSVGTYNVMLVVTTNLGCIDTIIQPVTINPLPVPAFTLLPGCAGTAVTLTNNTLDSVI
                     NWNWNFGNGQVSTQQNPTIVYSDSGTYNVSVTITDVFGCTGTANVPVTIEPAPVAAFT
                     FTGPTCIGDSFLLSNTSTGNIIAYLWNFGGTDTSNLQNPIVSFTTPGVHPVTLTVTSA
                     NGCISTYVQNITISSPPVANAGADAAICLGQSVTLTASGGLSYQWSNGQYTNPVNVSP
                     SQSTTYTVTVTDVNGCVAVDSVLVNINQLPVAVVSPPQSICFGDTATLTASGGVSYSW
                     SPVGLTGSVINVNPNSSSVYTVLVTDNNGCTANAQVNVTVNSNPVVNIPTTFVCTGTF
                     TTLNAGNAGSSYLWSTGDTTQTIDVTAPGNYAVTVINSFGCSSTDTVLVTPGGSITNN
                     QANISMCQGLTATLDAGNPGNSFLWSTGAVTQTITVSSSGQYAVTITNLNGCTATLIS
                     TVQVNPLPSAAITINDACINQAIQFFDSSSISAGNISNWQWSFGDSALSNVQNPTHNF
                     ANAGTYNISLVVTSAAGCMDSISQSITIFPKPVVAFSGVNACPGSAIQFQNSSSVSTG
                     SINAYHWNFGDGTTSSVLTPTHTYTSSGNYQVALTV"
BASE COUNT         8125 a         5151 c         5086 g         8907 t
ORIGIN      
        1 ataaaatagt ttacaatttt atttgttttt ctacggtata actgtcaggt cggccattcg
       61 ccccctgaca gttaaattct catagaccaa aaggttgatt acatacatcg taatccgttt
      121 tattgatgaa ccgcatttat ggaggttaag cagcgcaaac gaaaacagcc cgattaaggt
      181 gttaagtatg taagtgtttt ttatcggaag cgcagcttcc ctgcacaaac cccacgaagc
      241 acagcttcgc aggtacgggg aaggatgtca taatcattaa aaacatctcc taatacacca
      301 taacgtccct cagaaataaa aataaaactc gatttctttt taatcatctg cctctataaa
      361 gcagcgtata aaaaaaggct gcctcaattt attgttttga aacagcctct tttaattatt
      421 ttaaagtagg tttactttac cgactcaaca attgctttaa aagcgtccgg attgttaagt
      481 gcaaggtcag caagaacttt gcggttaagt tggtttcctg ctttatgcag tttacccata
      541 aactcgctgt aagacatatc atagtttctt acctcagcat taatacgctg aatccataag
      601 ccgcgaaagt tgcgtttctt tgttttacgg tcgcggtagg cgtattgcaa acctttttct
      661 acggtgtgtt tggcaacggt taatacgtta cctcttgcgc cccaatttcc tttggcgcgg
      721 ttgagcagtt ttttacgtct tgctcttgat gctactgaat ttaccgaacg tggcatgtgt
      781 tttaattttt ggtgttagcg ttccgccttg cggaatcttg atgctaatta cctggttaaa
      841 cttaattgaa taatattttt tatgctattg aaagcattct ttttatgtta aactcgtcag
      901 atttgtgaac gatggctgca ttggttaatg cacgcttgcg cttagttgac tttttggtaa
      961 ggatatggct tttgaaagca tgttttcttt ttattttacc cgttccagta acactgaatc
     1021 ttttctttgc actggagttt cttttcattt tcggcataac gcttctaatt tttatttatt
     1081 tattatttta tttacttctt ctttgcaagt ggtgccaggt gcagaaacat gcggttgcct
     1141 tctaatttcg gaagcatttc aacctttgca tattgctcca actcctgtgc aaacttcagg
     1201 aggataattt ctcctttttc tttgtaagct atgctacgtc ctttaaagtg aacataagct
     1261 ttaacttttg ctccttcttc gaggaatttg atggcatgtt ttaatttgaa attaaaatca
     1321 tgctcatcgg tattcggacc gaagcgtatt tcctttacaa cagatttagc tgtctttgct
     1381 ttttgttctt tgtccttctt ttttctttcg taaaggaatt tttgataatc aactatcttg
     1441 caaaccggag gattactggt atttgatatt tcaaccaaat caagacccat atcctgcgcc
     1501 atattcagcg cagtttgtgt atcataaatt ctgggttcaa cgttttcgcc aaccaatctt
     1561 acctgtggtg aatgaatacg accgttaatg cggtgctctt ctactttagg gcgtggtaat
     1621 cttctgtcag gaaaacgacc acctgtgggc gtatttggtt tgttaaatgt tgctatatag
     1681 cctccttttt acttgtttat taataattga tcttcaatct ctttttttac taattgacaa
     1741 aatgcctcag gtgtcatgct tcccttatct ccttcgccat gcttacgcac agatacctgt
     1801 ttttcattca tttctttctc ccccacaatc agcatgaacg gtactttttt aacttccgca
     1861 tctctgattt ttctacctat tttttcaccc ctttcatcaa cgagggcgcg aatatcgtaa
     1921 ttatttagta actgtgaaac agattttgca aaatcattaa acttttcgct tatgggcaga
     1981 atggctacct gcataggtgc cagccacaaa gggaaattac ctgcatagtg ctcaatcaaa
     2041 aatccgataa aacgctcatg tgttcccagc ggtgcacggt gtatgcaaag cggtgttttc
     2101 attgtattgt cagcatcacg ataggtaagt ccaaaacgtg caggaacagc aaagtcaacc
     2161 tgattggtgg ccaaagtaaa ttcacgacca atggcactcc aaacctgtac atctatctta
     2221 ggcccgtaaa aagcactttc atcaggtact tcaatatatg gaatattaga ttctatcaat
     2281 acgcgtctta ccatatcttc agtttttagc caaagttcgg gctcattaac atattttttt
     2341 cctaattttt caggcgaatg ggtcgaaaag cgcatgatgt atttctcaat tccgaagatt
     2401 ttaaaatact ttaagtacat gtcgttaact gccttaaatt cagaagcaaa ctgttcttcg
     2461 gtacaataaa tatgggcatc gttcatctga aggcaacgta cacgcatcaa tccaaacaat
     2521 tctccagatt gttcataacg ataacaggtg ccgtactcgg ccaatcgaat gggtaaatca
     2581 cggtagctgg cattcagatt tccaaaaatt ttatgatggt gcgggcagtt cattggcttc
     2641 agatagtact tttctccatc catttccatt ggcggaaaca tgctttctgc atagtatgga
     2701 agatgccctg tagttaaata catactttcc ttggctaaat gaggtgtacg aacacgcttg
     2761 tagcctgctt cattttcagt acgtttggcc agattttcca actgctcaat taagacaccg
     2821 ccattaggca gccataaagg caagccgggt ccaacttcat catcaaacat aaagatgtca
     2881 agttctttgc ctaactttct gtggtcgcgc tttttggcct cttccagcat tgccaggtat
     2941 tcatccaact ctttctgctt tggaaaagta atgccgtaaa tacgtgttag ctgtttgttt
     3001 ttttcatcac ctttccagta agccccggca aggttcatca acttaaccgc tttaatataa
     3061 cctgtatgtg gaatatgagg tccacgacat aagtcagtga aattaccctg tgtgtagaaa
     3121 gtgatattac catcttcaag gttctgaagt aagtctaatt tatactcatc atttttttcg
     3181 gtgaaataag cgatagcttc ctgcttggga acttcttttc tgatatagac attgtttttt
     3241 ctggcaagct caatcatttt gtcttcaatc ttcttaaagt catcgcttga aatttgcttg
     3301 ccgcctaagt ccacatcata ataaaagccg ttttcaacag gaggtccaac ccagaatttg
     3361 attcccggaa aataaaactc caacgcttct gccattaaat gtgccgatga atgccacatc
     3421 gtatttttcc catcctgact atcccatgtc aataattcta aagatgcatc attgtttata
     3481 ggtcttgaaa ggtctgacac ctgaccatta atttttgcag caagaacgtt gcgtgcaagc
     3541 ccttcgctga tgctcttggc tatatccatt gcggtggttc catcaggata ttctctgaca
     3601 tttttatccg gaaaagttat tttcattctt aaaaaatttg cgcaaagata attaactatc
     3661 catgtcgcta aaaaaaacag atatttctgt ttctgctttt ctgcttacat ttgcccatgc
     3721 accaaaccat actgatcatt gactttggct cacaatacac acaactaatt gccagaaggc
     3781 tgcgtgagct taatgtatat tgtgagattc atccttataa ccacctaccg gaaatgagtg
     3841 attctattaa aggagttatt ttttccggaa gcccattttc tgttcgggac agtctggctc
     3901 cttttattga tactaaaagt ttttttggta aaacacctgt gttgggcctg tgttacggag
     3961 cacaactact tgcacatcag catcagggtg aagtggtggc ttcaaaaatc agagaatatg
     4021 ggcgtgcaat tcttaaaatt aatttcgatg atgaaatttt taatgatgtc agcgacaaca
     4081 gtcaggtatg gatgtcgcat gcagacacca tacgcagtgc aggtaatgac tttgaagtca
     4141 tagcacagac tgatgatgtt gcggttgcag ctttcagaca taagcatttg gctgtttatg
     4201 gctttcaata tcaccccgaa gtttatcaca gcacggatgg taaaaaaatg ttggccaatt
     4261 ttgtctataa tatttgtggt tgccgtggcg attggacacc ttcgtcattc gtagatgaaa
     4321 cagttgcaca gttgcaagag aagttaaaga acgataaagt tattctcggg ctatcaggcg
     4381 gtgttgattc aagtgtggca gcattactgc ttcataaagc cataggaaaa aatctgtatt
     4441 gtatttttat tgataatggg ctgctacgaa aaaatgaagc acatcatgtt atggaagttt
     4501 tcaaaccatt agggttgaat ataaagtgtg tagatgcagc ctcagtattt tatgaagcat
     4561 taaaaaacat ttcggatccg gagcaaaaaa ggaaaaccat tggcagggta tttattgaag
     4621 tttttgataa agaagcgcat acaatttctg atgtaaaatg gttggctcag ggcactattt
     4681 atcctgatgt tattgagtct gtatctgtaa aaggaccttc ggcaacaatt aaatcacatc
     4741 ataatgtagg cggacttcct gaaaaaatga atcttaaaat tgttgagcca ttgcgatcac
     4801 tttttaaaga tgaagtacga aaggtgggca aggagatggg gttaaaagaa gagttgttga
     4861 accgtcaccc attcccgggt cccggattag gaattagaat attaggtgat ataagtgttg
     4921 aaaaggtgcg catgttacag gaagccgatc atgtttttat ttccttatta cacaaacata
     4981 acctctatca gaaagtgtgg caggcaggag ctattttctt acctgttaac tcagttggtg
     5041 ttatgggcga tgagcgtact tatgaaaatg ctatctgcat cagagcggtt accagtaccg
     5101 atggtatgac tgccgactgg gcacatctgc cttatgaatt tttaagtatt gtttctaacg
     5161 aaattatcaa taaggtaaag ggaataaaca gggttgttta tgacatcagt tcaaagccac
     5221 ctgcaacaat tgaatgggaa taggactatg gatttaagaa gcaacagaga ttatctgatt
     5281 tgggtattgg tctttgcgct gttgtcaggt tgcagcagca gcaggaaaac ttcgcaaatg
     5341 ccgccaccgg caccagcacc caagcctgct gaacaaccgg taaagaaacc tgaagttgtg
     5401 catgaaaaac ctgaaaagaa agagtttaga ataggtctga tgctaccatt aaatctggaa
     5461 caacagattc aaatgtttga tgatacttca gggaatgcca tacctgatta taataatttg
     5521 agcaaccttc aatttgtaga aggagcacag ttggctgctg acagtttaaa gcaattagga
     5581 atcaatgtta atttaaacat tgcagagacc cccgcagata gctttgcgct atggcgtttg
     5641 tggcggcaga acaattttgt taagtgcgat gctgtaatat cctctgtgcc gtcatctttt
     5701 attcctgctg ttacacgcct tagtgccaac agtaaacaaa ccctgatact tacacaacct
     5761 tcaaacacaa attttaaatc aactaacgag catgttttta tgctctcacc ttcgacatat
     5821 acacaatgta tggttgcagc taaagaaatt gcaacacgat ttcctgattt caagtatgtt
     5881 ttgcttgcac gcgatgtgaa gccggataaa gacattgcac agatttttaa ggatcatttg
     5941 cctgcaaagc atctgaaaca gtttatcagc agttctttta ttcccgattc agtttgtaaa
     6001 atactgaaat cggaaaaagc tgttctgata ataacatcat ctgccgaagc ttatgtcaat
     6061 accatactca atcaggtaaa tgcctgtata gaaaaacctg cattggcaat aggactgcct
     6121 acatgggaaa attttgaaag tatagacttc agcgctctta agaatttacg cattgccttt
     6181 tttacatcaa catatttaga ttaccatgct gcttctacca aaagtttcag aaatagtttt
     6241 attaaccatt acaagacaga accattcata tcagcatttc agggttacga agctgtaatg
     6301 gaatatgtga aatcaaatac cgggcatggc tctttaaagt cgggctattt accaactcta
     6361 aatataaaat acgcgaccga tacagctaat ggtataaaag aaaatcaggg gatcaatctt
     6421 atagaaatta aagattatac attaatacca ttagaatagt acaatgtatt tcaatttaaa
     6481 ttgctgattt gtagttattt atattgtgaa gtagagaaag ggtcgctgta gtaaaaaatt
     6541 atgaaaagca tttggcatta aacaaaaaat aattactttt gcactccctt aataaaattg
     6601 agtagttatg tcccgtatat gtgatttgac cggaaagaca agtattgttg gaaacaatat
     6661 ttcattttcc aatcgtaaaa caaagcgcag attcaatccg aatttgcaaa ctaaaaagtt
     6721 ttttattccg gaaaccggag agtgggtaac tttaaaagta tcagcttctg ctatccgtac
     6781 catcaataaa aaaggaattt cagcctgcat aaacagtctg ataaaaaaag gacacattta
     6841 atcttttagc atcatggcaa aaaaaggaaa cagagttcag gttatcatgg agtgcacaga
     6901 gcataagaac agcggtatgc ccggaacatc acgttatatc acaacaaaga acaagaagaa
     6961 cacaacagag cgtctggagt tgaaaaaata caaccccatt ctgaaaaaag ttacgcttca
     7021 taaagaaatt aaataattta aagcttaaat aaaatggcta agaaagtagt tgcaacactt
     7081 aaaaccggta aaggaaaaga gttttccaag gtgattaaaa tggtgaaagg tgccaatgga
     7141 gcctattcat tcaaagaaga gattgtgcat aacgatcacg taaaagattt tttgaaagag
     7201 aaataatatt tatgaaatcg aaaaacagct ttcttccttc aggaagaagg ctgtttttgt
     7261 tttaatacta caacaaaaag aatcagacta tgggattttt cggactgttt tcgaaaaaag
     7321 ataaagaatc acttgacagc ggattaaata aaactcgtga gaactttttt acaaaaatta
     7381 ccaaagcagt aattggtaaa tcaactgttg acgatgaagt gctcgataat gttgaggaga
     7441 ttttagtatc ttctgatgtt ggagtagata caacattaaa aattgtaaaa cgaatacagg
     7501 atagggttgc acgtgataaa tatgtgggaa ccgatcagtt gaacacgatt ttaaaagaag
     7561 aaatagcagc attacttgcc gaaaacaagt cggctactgt agcaaatctt attgatgaaa
     7621 gcaaaaagcc ttatatcatt atggttgtag gtgtgaatgg agtaggcaag acaacaacca
     7681 ttggaaaact ggcatatcag tttaaacaat caggtaaaaa tgtgttgttg ggtgctgctg
     7741 atacttttcg tgctgctgca gtagatcagt taaacatttg ggcagagcgt gttcaggtgc
     7801 caattgtttc gcagggaatg aatgctgatc ctgcttcagt tgcatttgat actgtaaagt
     7861 caggtgttgc tcaaaacagt gatgtggtaa ttattgatac agcaggacgc ctgcataata
     7921 aagttaacct catgaatgag ttaacaaaaa taaaacgtgt aatgcagaaa gttgtgcccg
     7981 atgcaccaca tgaaatattg ttggtgcttg atgcttcaac aggtcagaat gccattgagc
     8041 aggcaagaca gtttactgca gcaacagaag tcaatgcact tgcactcaca aaattagatg
     8101 gtactgcaaa aggtggtgta gttattggta tttctgatga gtttaaaata cctgtaaaat
     8161 atattggagt aggagagaag attaccgacc ttcaggcttt tgacagagtt agttttgttg
     8221 attctctttt tgcaaaatag tacggccgct atttaatttc ttttaactcc tgccattttt
     8281 ggtcgttcat attatcatca aggtttggat tgtaatcaac tttatcggta ctgatatatt
     8341 cgccatcgtt attcatccag tattgattgt aaccactttc aactttatac ttctgcccac
     8401 tttcatccac aacattggtt ctttcataaa ttccatctat acgttgttct tgcattctgt
     8461 ctgatgcagc atttctttcg cgccagccac tcataatggc atcgtttatt gcatttgttt
     8521 tattgatgtg cgcacgttgt gatgcttcaa aatttgcctg attttgtgcc atccttgcat
     8581 taaactgcgc ccaacttcta ccggcttttt cagcttcaga tctgttatat gccattatag
     8641 gctcaagttc ataacgtaca tttgaaatcg aaaacaagag ttgcttcttt gctttttcaa
     8701 aaacatcctt gtctgcttca aggctggata caaaataact ccacatacgc agttgctcag
     8761 ttgtgctgat tgaaaaatgt actattaaaa agtatggatt gccatcagca gtttgccaat
     8821 ctgttccaat agcaaatgtt tttttgtcat tgggtacagc ctgatataat tgatcactat
     8881 accatttctc gatacgcgat acgtccggca actcataata ttttatgaat gaaagaccct
     8941 gattgcggca aacaggaatt aaatcttgtt gaataacttg ttctacaccc ggaaaatctc
     9001 taacctgttg cccgctttga ctatagagat actgtgtgtt ttggtcaaaa ggataattga
     9061 acatctgaat cggataatct ttaacaacaa ttccatcagg tcctgtaaag gtaggatcgc
     9121 ctttttgcct gatgttatgt attttccagt ttgagggtaa gggaattgta gatactaaat
     9181 tgccattagc atcacgattt tcaactgcaa ctaatttttc ccaattgctg tttttacttg
     9241 aggaattttt atcagtcgcc tgtgatgttg tacttgtttc tgtttcttca tcagtttcct
     9301 gtccggaaga gttaccattg ctgcaagatg caataattga aatgagtaat ccgcatccaa
     9361 tcaatcttaa agttctcatc ttttattagt ttaataaaat gtaaaactaa tcatatcaga
     9421 taaaatcacc aatcaccaat aatggtgata tgaaacaaaa attactgatt tgatgttatc
     9481 tgaaaaataa gattgataaa gaccaaagaa aattaaaaga ctacaatatg ctgtctataa
     9541 ttttttcgac tgaaacacca tcagcttcgg ctttataatt acgtactaaa cgatggcgta
     9601 gaattggaac ggcaactgct tttacatctt caatatcagg agaatactta ccattaattg
     9661 ctgcatgaca tttagcacct ataactaaaa actgtgatgc acgcggacct gcaccccatg
     9721 caacataatc gtttatcata acagatttgt ttgctgcccc cggacgcgtt ttggctgtta
     9781 gtccaacggc ataattcaat acattatcag ctacaggaat tttacgaatc agttcctgaa
     9841 aaaaagtaat ttcattttcc tcaagtactt tctccacttt accaatttta tctcctgtgg
     9901 tgttttttac tacctgaact tcttcttcaa acgaaggata gtcgaggaaa acgttaaaca
     9961 taaatcggtc gagttgtgct tcaggaaggg gataagtacc ttcttgttcg attgggtttt
    10021 gtgttgccaa aacaaaaaac ggaataggca aatcatacct ttttccggaa acggttacag
    10081 acctttcctg catggcttca agaagtgctg cctgcgtttt aggaggtgta cggttaatct
    10141 catcggccag aataatattg gcaaagacgg gtcctttgat aaatttaaag tgtctgtttt
    10201 cgtctaatat ttcaatccct gtaatatcac ttggcattaa atcgggagta aactgaatgc
    10261 ggttgaagga aagacctaaa gcttgtgaaa tagtgtttac taaaagcgtt tttgctaaac
    10321 cgggtacgcc aacaagaagg cagtgtcctt tgctgaaaat agaaataagt acatcgcgta
    10381 caactgcatc ctgcccaaca attacttttc caatctctgt tctcagtttc tgatagctgt
    10441 tgactaagtc ttttattgct tcaacatctg aagtatagtg ttgattcatt ttgaatattt
    10501 tttatgttag tttgaccatt tttttatctc ttcgcaatga ctgtattcag ggtcaattct
    10561 cacataggtt gacttcctct ttctcacaat ccagtcatcc atcaatttag cttgtttatg
    10621 acttaatgca gcctgctgaa tacgttgata atcgtcagtc agatttgcaa cgtgtggttg
    10681 tgtgcgtttt ttaagcagaa aaattttata atactgtgag ccatcgcgtg aagtgattaa
    10741 tgcaggatta ctcacttcgc cttctttaag tttgtcaagc tgaaaaaaca ccatagcatc
    10801 tacctgattg gcttcaaaac gtgtattgcc tgtagcgggg ttcaccacat tgccgccatt
    10861 gttttttgaa tcttctgcat ctgaaaattg tattgctgca tcttcaaatt ttatttttcc
    10921 gcttctgata tcaaatgcaa tactatctgc tttagctttt gtggctaaca aatctttatt
    10981 gctgacctca ggtttcagta atatgtgacg tacattgatt gtttctcctc tgcgatcaat
    11041 taattgcaca acatgatagc cgaattttgt ttctatgatt ggtgaaattt ctcctttttt
    11101 caatttataa gcaatggctt caaactccgg aacaaactgt ccacgtggca caaaacctaa
    11161 ttctccacca ttctttgcag agcctgcatc ctgcgaataa agaattgcaa gtgttgcaaa
    11221 atcttcacct tttaacaagc gggttctgat ttcttcaagt ctggttttaa ctttattttt
    11281 ttcttcatcg gagacagcaa ttttaaatgc aatctcacca tattccaatt cggaattaat
    11341 gtatggaagg ctgtctttgg gtatggaatt aaaatatgct ttgacatcgg caggtgatgc
    11401 agatgcctta ccaattattt tttgttgcat ggtttgagca agtaactgct ctttaactaa
    11461 aggacgaaac tcatctttaa tttccggaac agatttctga taatattctt ccaacttttc
    11521 tgttgagccg ttaaactgtt cagtaaaata ggcaatgcgc ttttcaattt caccttctac
    11581 ctgtgcatcg ctcactgtaa cgctgtcaat attggcgtaa tgaataagta atttggtcaa
    11641 caacaactga tccagtacat cgcattttgc agactcatct ttataatgac cttgtgcaag
    11701 atactgttgg tattgtgcct caaggtcaga ttgtaaaatg attttatttc caactatacc
    11761 aatgatctga tctatgacag tgtcggattt ttgtgcatac aacagtgatg gcataatgaa
    11821 taccatcaat aggagaaatg ttctatttat atatttcaaa atcattattt ttaagtgctt
    11881 gatcaaatgc agatttctgc atttgctcta tgagtgcaag ttttcttttg ttaataatca
    11941 ggttttttat attgtctttt acaaaggcaa gaggcgaaac gttttcatta gttttgaagt
    12001 cagtaatttt tacaaggtaa atgtttgcag aatcagcagt ttcaatattt ctgttgttac
    12061 gcaaaaactc ttctttgtca tatgttttaa tcggaactct tttcaataaa tcatcaaact
    12121 gcatccagtc gttgtcgttt agcgaatagt caatggcata ttgaaaacaa aattcttcaa
    12181 gaagttgtct gtctttttct tttgaagaat taaaccagga tttaagttta tcaatcttcg
    12241 gtgttttctt agctgcttta atgaaagttg ctttgataat attactcttc aacaaaaagt
    12301 ttttactgtt atcgttgtag tattgttcaa tttcctgatc cgtaactgtt gtgtcgagtc
    12361 gctggcggac caactcctgt tcgtaggcaa aagtgattaa cgagttacga tattcctgca
    12421 attgcttctc tacatttttt tgttcatcaa ggagattgtt ttctgcctga tgcaatacaa
    12481 tattttgttt tatccaatcg tccacaaaac tttttacaat cattgcgctg tcttgcggtg
    12541 tggcatcttt aggcacaagg tctttaattt cgtaagaata taaatacttg tcataaacgc
    12601 gggcaatagc atttttattc ttatcctttt tgaagtcaac aggcagattg ttacaactca
    12661 ttaaaaaaac agttccggca aatgctaaaa gcaagccgga accattaaat gttatgtgtt
    12721 gttttttcaa tatgcagttt cttacttggc aaacatagat tttaaaacat cctcgtttac
    12781 tgaatagctg tattttttac gcagttcagt tatccatgat ttttcaagaa aattctgata
    12841 gtcggcagtt attaatccac gtgcttcgtc taatttttta ggctgtactg caagtttaga
    12901 ttttacatct gcaaaaacca cctgattgtt gtctttcatg tcagccgata aacccggttt
    12961 ccattcaact ttatcaatga tttcattatc acctttagaa tattttccgt ctttaatgtt
    13021 tatgttcaat gatgaatctt tatttaaagt tgcagctatt tcactgtcag attttttctt
    13081 tttaatcatc tttcttgcct cttttgcaac atcagcattt ttacaagtgt atattacagc
    13141 atctaagcgc tcaccccaca tgtagttgtt tttgtttttc tcataaaact gttctaatcc
    13201 tgaggtgtct ttcacagctt tgctccaaac cattttatca gtaaggtcga atagcaaaat
    13261 accatctctg tattcatcca tcaatgcttt gaaatcagga tattttttat ctaactgcga
    13321 ttcttcaagt gcaatacatg catctgttac aaaattttcg tattgtgaat gtgctattgc
    13381 ctgcgaggtt atgtcttttc tttttgtctg atgttttgca agataagccg caaaatcaaa
    13441 ttgcgtataa tctttacctg ccaaattaaa caaaggcttt gtttttttaa ttgcagttga
    13501 gtcgttatag gtaccatcgg caagcgttgt gtcaagcgat ttaatatatt cgtctttgtt
    13561 agcagcaaat tctttaaagt tgttttcttt cttgatttta gcaactaaag atgctttgcc
    13621 catttcattg cggctgtcac gcataatctg atttttcagt tcatttttct tttcatcgaa
    13681 tgatgcaata cctcgttttt ctaaacgctt tatgatatgc catccataag cagatttaat
    13741 cggtgcagaa acatcgttgt cttttaacaa ggcaaaagat gctttttcat attcaggaac
    13801 cattttacct gtgccgaacc atggaagtac accgccattt acagcagagc ctttatcatc
    13861 agaatattgt ttggctaaat cttcaaactt ttcacctgcc ttgaacttag tatagatttc
    13921 atcaatctta cgcttagctt ctttttgtac ggagtcatta tcacttttat taaccttaat
    13981 catgatgtgt gcagtgtgta tttctccaac agcaggacga atatcactaa cttttataat
    14041 atgatatcca aagcgtgttc tcacaggcat tgacacctga ccgggtttag tattgtaggc
    14101 tgcagtttca aaagggtaaa ccatttgtaa gcctgtaaaa taaccaaggt cgccaccatt
    14161 atcttttgca gaaggatctt ctgaactatc gcgggccatc ttttcaaaat ctgcaccttt
    14221 tacaatcttt tctctgattt tcatgattct gttatatgca gccaaagtgt cttttggtaa
    14281 agcatcactg cttaatttaa tcagaatatg gcttgctctg acatcgtttt gcagtcggtt
    14341 atatgcttcc ttaactaagt tgtcattcac atctttgtcg gtcaggtagg gttgagccaa
    14401 ttgcttacgg tagccggtta gctcatcctt aaaagctgca gaagtatcca ttttaagaga
    14461 ttcggcttct ttaactttta atttgtagtt gataaaaagc tccatataat ctctgatgga
    14521 tttttcatcg ttagcggtgc ctttattatt gtttttgtaa taaacccttt cgaattctga
    14581 gcgggtgatg ttttgcccgg caacatttac taaaacaggg tcgctgattg caacaggtgc
    14641 agtaacagct tttgtgggtt ttaccgttgc actgttagtt ttcttttgtg cataggcatt
    14701 tgtaaaaaag caggaaatta aaataattcc caatgcgtta aagtttttca ttgtcttgaa
    14761 atttaaggag gtgcaaattt aaaaaaatag attaaactgc taagccgggc taaaatagac
    14821 ggttacttat gaatcaaaaa tgaaagtcag gaaaatgcgc ttagtcgtac gactcaaact
    14881 taatggcttt tttatcatat cccatagctt ctaagcgctg acgtgcttct ttcagcatat
    14941 cagcccagcc acagataaaa aactttgctg gacgtttatc ggcaaaaatc tcctcatata
    15001 cctgatgcac ataaccttta cgaccagtcc agccttcatt atcacgagat agaacaggga
    15061 taaatttaaa ttgaggataa tctttttcta actgctccat ttcttgtctg taaaggatgt
    15121 ctttagtcca gcggttgcca aaaaccatgt ataagttttt atgcggaatt ttacgattaa
    15181 gtatgtcaat aacctgtgaa cgtaatggag caattccagt gcctgtacag ataaaacaaa
    15241 ggtcggtatc aataggttca ggaatctgaa acttacctaa aactcttgaa agctttactg
    15301 tagttccaac tttataattc tcccacatat agggagtacc cattcctttg ggatttagaa
    15361 ctatacaaag ttcaaaaata ttgtctgttg aaggtgctga tgcaatagaa tagctcctgt
    15421 tagtatattt agaatcaata ggcaaatcaa gcatcacaaa ctgtccggct ttaaaactga
    15481 atggacgatc atccggtact tttataaaat atcgttttac tacatcgttc tcatcaatta
    15541 tgtcaacaat tgttgcatcg taaaattcgt aagccataaa ttctttgtgt tagaatgatt
    15601 aaattttcgc gaccaaaata aataaaatca caaaacaaaa agcgataaaa aaacggtacc
    15661 ctaattaaag aataccgtca ttaaccatat acaatgaaaa aatcatccta aataaacctt
    15721 cagaatttta cttcttgagg tatgtttcaa tctgcgaata gccttctctt ttatctgtct
    15781 tactctttcg cgtgtaagtt caaatctttc tccaatttct tcaagtgtca ttgcaggttc
    15841 gggagtcagt ccgaaatata gcctgatgac atcagcttcg cggggtgtta aagtggttag
    15901 tgcgcgttca atttctcttt taagtgattc gctcatcagc gaaacttcag ggccttgtcc
    15961 gtcttctgta gggattaaat caagcatatt gccatcttct ccctgtaaga gtggtgcatc
    16021 cattgaaata tgttttccgg ccgaacgaag tgtgtcgttt atttcatcaa gattggcatg
    16081 tagcacattt gcaatttctt cgtttgatgg ttctctttca aaatcctgct caagttgtgc
    16141 atatgtcttg ttaatcttat tgataaaacc aagtttgttt acaggcagtc tgacaatgcg
    16201 agcctgttct gaaatagctt gtagaatact ttggcgaatc caccaaacag catacgaaat
    16261 gaatttaaaa ccacgggttt catcaaaacg ataagctgct tttataagac caaggttgcc
    16321 ttcgttaatt aagtcgccaa gagtcagacc ttgattctga tattgtttag caacagaaac
    16381 tacaaatcga aggttggatt ttgtcagcat ttctaatgca tgttgatcgc cttcttttat
    16441 ttttcgtgca tatttgactt cttcttccga tgtcagtaat ggcaatttac caatctcgct
    16501 gaggtacttg tctaatgagt aggtttcgcg gttggtaact tgtttcgtga tttttaactg
    16561 acgcatagtt taatagttct cctgaaaata tttatgaata gtgaaacgca actacacaat
    16621 tacggttaca cgacagagaa aaggtatttg tatgaaaagc ggaatatttt caattattct
    16681 accacaatca ttgagaagta aaaagaataa gtgcgattgt tttatgacaa aaaccttaac
    16741 aacacctctg aaaaacagga atttgcagag aaatgtaaaa ggcgaggttt gatgaaaaaa
    16801 ttatgtaatg aaatcagaaa aaaaatcttt ttgcacatgt tttttaaatt tgcggtgaat
    16861 gaaacctgaa ttagaacaag aaggcagtgc acaggagcat ctggagcagg acgaactctt
    16921 tgaacatcac cgcatagtag ttgataatgg gcaagtgcag atgagaatag ataagtttct
    16981 cacagcaagg atggccaacg ttacccgcaa cagattgcag gtagccatag atagtggaag
    17041 tattctggta aatgatctgc cgactaaggc aaattataag gttaagcccg gagatatgat
    17101 cagggttgtt ttgccgtatc cggttcgcga aatagaattg ttgtgtgaag atattccatt
    17161 gaatattgtc tatgaggata agtatcttgc agtagttaat aagcctgccg gcatggtggt
    17221 gcaccctgcc tatggtaact acacaggtac tttatcaaat gcaatggcag ggcattttta
    17281 tcctgagttt aaaggccagg ctttgtcaaa agatgttttt aagcccggca tggcgcatcg
    17341 cattgataaa aacacatcag gattattggt tgttgccaaa gatgaggata cacttaacta
    17401 tctggccttg caattttatg aacgcaccat taatagaaat tatattgcat tggcatgggg
    17461 agatataaaa caacaaaaag gtgaaattga gggtcatgta gggagaaaca aaattaacag
    17521 aaaagttatg gatgtttttc ctgatggcag ctacggcaag tatgccaaga cacatttcga
    17581 ggttattgag cgttttggtt atgtaacact aattaagcta aaattagaaa caggaagaac
    17641 acatcagatt cgggtacaca tgcagcatat cggccatcct ttgtttggcg attttgaata
    17701 tggaggagat aagattctga agggaacaac agaaaataag tataagtctt ttatacataa
    17761 ttgttttgaa ctcatgccat atcaggcttt acatgcacag tcgcttggtt ttatgcatcc
    17821 ggtcaacggt gagataaaat tttttgaagc agatttgccg gagaatttta agcaattgtt
    17881 agaaaaatgg cgtaattata catcatcaac acatcgcgat ggatgaatga ccggactaca
    17941 aaggctcatt attttcagaa agaatatatt tgcctgaaat ctttatgcgt gtttttgttg
    18001 aatgattaat gccgagaaca tcactctatt attagcagcc tacttattgg gctctattcc
    18061 atcagcagtg tggattggta aagtgttttt caatactgat gttcgcgaat atggaagcgg
    18121 taatgcaggt gcaacaaata cttttcgagt attagggcca aaagctggtg tgcctgtatt
    18181 gttgatggat attgcaaaag gttggatggc agtacaatta gcaacttatt ttggagaata
    18241 tatgccggca acacaacagt tcattaattt taagctaacg ttgggtgtgg ccgctttatt
    18301 aggacatatt tttcctgtat tcgcaggttt cagaggaggt aaaggggttg caacattatt
    18361 aggtatccta tggggcgtga acccacctgc tgctgtaatt tgtatgggtg tatttttact
    18421 tttcttttta acaacaggct atgtttcatt aggctcaatg gctgctgctg tttcttttcc
    18481 atttattgta atgtggtggc taaatgaaac agtaacgagt atgaatgttt ttgcaatagc
    18541 agtgcctatt ttggtgttag ttacacatca gaaaaatatt gaacgccttc ttaaaaaaac
    18601 agagagcaga gtttatcttt tcaggcgtaa acaatagaaa ctttagcacc tttcaataat
    18661 tacagcataa ccttgtccaa caccgataca catggtgcag agggcatatt gctttttagt
    18721 aagcgttaac tgatgtgctg ctgtaagaat aattcgtgct cctgacatgc ctaatgggtg
    18781 acctattgca atggcaccac cattaggatt gatacgttca tcacgatcat ctaaattcca
    18841 ttgtctgata catgctaatg cttgtgctgc aaatgcctca ttaagttcaa tgatatcaat
    18901 attattccaa ttgagtcctg ccttttttaa tgcagcattt gctgcgggta caggcccaat
    18961 acccattatt ctgggttcaa cacctgctac tgccgaagac actatacgtg ccataggctt
    19021 taatttattc tgagtaagtg tatcttcatc agttaaaaga attgcacatg caccgtcatt
    19081 aacaccggaa gcatttcctg ccgtaacact tccttctttt ttaaatgcag gttttaattt
    19141 ggataaaatt tcaatgcttg tatcgggttt gatgaattca tcaatagagg caacaatagg
    19201 gtcacctttt ttgcgaggaa tgattacagg agagatttca cattcaaacc tgccttcacg
    19261 ttgtgatttg gcagctttct tttgtgcatt taatgcaaaa agatcctgat cttctctgtt
    19321 gattttatat tgtgctgcaa cattctctgc ggtttcgccc attgagtcgg tgccatacat
    19381 ttccttcatg cgtacattaa caaaacgcca gccaatactt gtgtcaaaaa gttgaacatc
    19441 acgtgcaaaa gctgatgatg attttgacat aacataagga gctctgctca tgctttcaac
    19501 gccacctgca atcatacatg aggtatctcc tgttctaatt gctcttgaag catttattac
    19561 tgctgacatt cctgaagcac ataggcgatt aatagtttca ccgggcacag taacaggtaa
    19621 tcctgcaagt aataaagcca ttcttgccac atcacggttg tcttcaccgg cttgattagc
    19681 gcaacctaaa attacatctt caattttttc aggcgcaagt ccggaatttc ttgccatcaa
    19741 ctccttaata acatgtgctg ccatatcatc aggtcgtact gaagatagta tgcctccata
    19801 atttcctaca ggcgtgcgaa tgccatcaac tatatatgct tgtttcattc agttatttaa
    19861 aatattcttt acttgttctg taaacagtgc ctttgaaaaa tgcaacctta atattatttt
    19921 gatttgaaat agtaatctga taaacaccaa ttctgtttgt cagcgaaagt tcttcggtgg
    19981 tagcggtaat tatatcgcca ttgtgtacag gcacaggata agatattgaa cattccaatg
    20041 caacagacaa tcttccatag gcattggaag caaaagcaag cgcactgtcg gctagcgaaa
    20101 atgcaatgcc tccatgaatg agtccgaagc cattcagcat ttcttcgcgt atctgcattt
    20161 gaagtacact ttttccgggc tccattacta agcattttat tcccaaccat tgactgaaac
    20221 gatcgtgttc catcatgtgg ttgactactt tttgggcatg tgttgtctga tccataaatg
    20281 caaaggtaat gattgatgag taggaatatt ttccggaata agatctgttg tcaacaacac
    20341 acttatttct tttccaatgc accaatatct ggggcagcac cttgtggtct ggtatttccg
    20401 tcaatatcgg tgttgagttc aggaaatgtc agcaatgaac catcgcctgc atcaatagca
    20461 ggagagttgt cctttaaaat aaaattatac tttgactgat cttcaaataa tggatcttga
    20521 tttttaaggg tattgataaa agagggaatc acacttatgg gagtaatttt ttcatcaacc
    20581 tttatgaggt tatttttaaa agagtatgtg tccgcaagac tatcaagtaa atccttttca
    20641 atttcattgt cgttaccacc ataaataata caattgtcaa cggtgatgtt tagtggaata
    20701 tgcacattac tgcttccatc attcacaaaa tcattcataa aaaatgcagg tgtactacgt
    20761 tgcgaatagc gccaatagtt tgcaatggtg ttttgtttca ggttataagt tccaccttgc
    20821 gagaatacag tacaatagct cttgcagtcg cttataatat tgttataggc agtgatatta
    20881 aaacttctcg ccagtaaccc tatactcata tttcggatga tgctgttact taatgttagc
    20941 tgcgtaggtg cgtcaccaat gactccatca tactgaacat cttctgcctg aacaccaaat
    21001 aaaccattct ttataatagc gtaattgatt ttatttgttc caccttcatt aatccagatt
    21061 ctgtcccact gattgggtac ttcgctatat gacgattcta atcttgagcc agtaaacaat
    21121 accggatgtt ctaagtttcc ttctacttgc aaattaccat aacgataaac ccacaaaccg
    21181 gagttgttat aaaaataaac ttttgtgccg gcattaattt ttagagtaag acaactatca
    21241 accacagcat agccatacac cacatagggt aaaagactgt cccattctat aactgttcca
    21301 caaggtgagc tttctccaat aatagaatag gggagtccgc tgccatcagc aaatgtaatg
    21361 gcatgtttgg gtgtgatgaa gtgcgcattt ctgccataag caaccagatc tacatcctga
    21421 atatttccat tggtttcaaa gattatagaa tctgtgacaa taaagggcat caggtcgctt
    21481 gtaggatcaa tagttacttc aataaaaatc caaagactgt ccttagcacg aatttctata
    21541 tcgttaagac tgttggctgc tacaccatca acattcattc tgaacggaga ggaactacct
    21601 cctgctaatt ttatagaaga aatttttatc ggacgattat gatcgttatg gacaagaagg
    21661 tattgtgtgg tgctgcctat agatgcaaaa acagtatcga ataaaacagt atcttcagaa
    21721 aagttgagct ttgctgatga atcggtcaac aactgatctt tcctacaggc aataagtaca
    21781 acaaataata gcaataagaa tattttgaaa aaattgtttt tatacatgtt tttttggcta
    21841 atttaaaagg atgaagtttg atacccctca attaaatatt ttctcattaa taaaacagac
    21901 gagttattta cttgaagtag catttccgat aactaatcca acattaaaat aaaatgtacc
    21961 gtatcgggct tcggatttgt aatatgccgt attatcgagt gcatcagaaa ggccgatact
    22021 ataacctaaa tcaaaagata ttttccagaa atcaacacca agtccagcat caaaattaaa
    22081 aacaccattg ttgaaattat cttcaagttc accaagagtt actttgtcca aatcatcact
    22141 cttggcactt ttagaaataa gaacattgta agacgggcca atgctgaatc tgatttttgc
    22201 attttcatca tcaagaagat tcagtccaag acgagcatta agtttaatat cgctgcgttg
    22261 aagatctgtt tgaattctta aagaatcatt gatgaaagct gtagaagttt gccaataata
    22321 aattccgggt tgaacataaa atcgtctgcc tattctgaaa tcggcaccgg catgccatcc
    22381 cacccttgct ttggtagatt cttcactttc cagattccta agctttgcaa ccgacaaacc
    22441 aatctgagga ttgaatgcaa actgcgcaaa tgatgtcata ctactcaaaa gtgtgaccac
    22501 caataaaaaa attcttttta aagtcataag attgaaacga taaaagtaaa aaaatcttat
    22561 caatcctaag gaatttcaga aaaataagtt gttaactttc taaaactttt cctgtttcca
    22621 attttataat tcttgcagga aaacgctcaa tcaacgagta attatgtgta gccattatca
    22681 ctgaacatcg tgttttgctg agttcaaaaa ataagttcat gatttcatta cttgtttcag
    22741 ggtcgagatt acccgatggt tcatcagcaa gtataagttc aggctcgtta agtaatgcac
    22801 gtgcaatggc taagcgttgt tgttcgcctc ctgaaagttc atgtggcatt ttaaatccct
    22861 tggtacccag tccaactttt tgtaaaactt cgtttattct atcatctgtt ttttgtttgt
    22921 tgttccagcc tgtggctttt aaaacaaaaa gcagattgtc ttttgcagtg cggtcaccaa
    22981 gcaattggaa atcctgaaat acgataccta actttcttct taaaaacgga atttctttat
    23041 tcttgagttt gttcaggcta tatcctgcca cattaccttc gccctgattt aatttaatat
    23101 caccaaacag tacacgcaat aatgaactct ttccgctccc tgttttaccg ataagataaa
    23161 cgaattcgtt tttctgcaca tgcaggttta cgcctgtgag aatcagattc tttttaacgt
    23221 aaatgttggc ttcctgaagt gaaacaatag tatctgtcat aattctattt tttgaacttt
    23281 accaaatgga atttcagcaa ttatttttaa aagatattct tctttatccg atagtccggc
    23341 tttagcaata gctttttcgg gcctgtctgc tctgaaatag gcaatgagtg taaaattatc
    23401 atcccgcatt tgaacataat cagggatttt ggccgtgcct ttaattttaa gaagatacat
    23461 tgtggcaaat gtaaatagtc gttttgctta aagatttaag taacccaagt aattatacac
    23521 tttcgtttgt tcaagaaagc atattgcttg gccgctgttg ataatagaat aatccttcgt
    23581 gataagttag agaaatcaaa ggtgaagtgt aatttttatg aagaattatg ggcagtgcaa
    23641 tcttttttat tggctttaaa agtttaataa tattttctta catcaaattc tgattggtat
    23701 taaaggtcag gaacaatttt tactttgtat gagatgtttt ttagaggtga ttttttgaca
    23761 aaaaaattaa aaattcttac aaaactacca aacatacaat tttcatttgc ggtcactttt
    23821 aaagggaaat aattttgatg gtatcaatag aaaagtgcca gaagtctatt gacataacat
    23881 caaaatctgt tgcctatgcg caagaattta ctcaacattt ttcttacttt gctgatagct
    23941 gccggtatgc actacaaaag tatggctaca cacagcatgg gtgccgatct gacctacgaa
    24001 tgtgtaggcg gaaacaatta taaaatcagg gtagcttttt atcgggattg cattggaatt
    24061 aatgcaccta acagtgtgaa tgttacaatt agttctgctt catgcggaca acgctttaca
    24121 cgcacacttt ttcctgttcc gggaacaggg caggaaatta caccactttg tcctaatgca
    24181 ctatcaacat gtaaaggcgg ggtatttacc ggtattcagg aatgggttta tgaaagagta
    24241 gtgacactgc cggcacaatg caccgactgg acttttagtt atgaactttg ttgtagaaat
    24301 gcagcaatca ataccattac taatccattg acaaatactt tttacatcta tgcaacttta
    24361 aacaatacaa tttcaccttg taatacatca ccggtgttta gtaacaagcc ggttcctttt
    24421 gcatgcttgg gtcaggaatt atgttttaat catggtgcgt ctgatgcaga tggcgattca
    24481 ttggtttatg agctaattac accaaaacaa aatgattcta ctgatgtaag ttatattcca
    24541 ccattcagtg catcaaatcc tctgacatca agtccttctg tttatttcaa cacgtcaacc
    24601 ggtgatattt gtatgacacc aacacaactt gacgtgacag ttatggctgt gctagttaag
    24661 gagtatagaa atggtgtcct gatcggaact gttgaacgtg atattcaagt aacagtaatc
    24721 aattgtaata atactttacc tacattatca ggaataaacg gcaccgacca gttctctgat
    24781 actatctgtg ccaatcagcc atattgtttt aatgtttatt ctaatgaccc tgatgcaggg
    24841 cagaatctaa ctgttgttta tgacagttca gtgaccggtg ctacttttac aacagcagga
    24901 actccacatc caacaggaac tttctgctgg acgccaacaa attcagacat cagtggagcg
    24961 ccaagatgct ttacaattaa agttacggat gataactgcc cctggcttgg tagtcagatt
    25021 tattcctatt gtttaaccgt agttggagtt aatgtaaatg ccggtccgga tcagtctatt
    25081 acctgtagtg atcttgcaac agttacagca acagcttcag gaggaacagg agggccatat
    25141 acctatcaat ggagtaatgg atttacgaat ccaacacaaa ctgtcgggcc gggaacttat
    25201 atagtaactg tcagcgatgg tatgtgttct gcaaccgaca ccgtagttat tcagtatatc
    25261 tctttcccta cagcagcctt cactgcttca tctgcttgtt tgaatcaggc aattactttc
    25321 actgatcagt caacagtttc aggtagtagc attatagatt ggttttggga ttttggtgat
    25381 ggaaatacag ctactgttca aaacccaata catttgtatg atagtgttgg cacttataac
    25441 gtaatgctgg tcgtaactac caaccttgga tgtatagaca caataattca accggtaaca
    25501 ataaacccac tgcctgttcc ggcatttacg ctgttgcccg gttgtgcagg taccgcagtt
    25561 acattaacca ataatacttt ggatagtgtt ataaactgga attggaattt tggaaatgga
    25621 caagtttcaa ctcagcaaaa tcctacaatt gtttattccg attcaggaac ctataatgta
    25681 agtgttacca tcacagatgt atttggatgt acgggtacag ccaacgttcc tgtgactata
    25741 gaacctgcac ctgttgcagc atttacattt acaggaccaa catgtattgg cgatagcttc
    25801 ttgcttagca atacttcaac cggaaatatt attgcttact tatggaattt tggtggaaca
    25861 gatacgtcaa acctgcaaaa tccaattgtc agctttacaa ctcctggtgt tcatcctgta
    25921 acattaactg tgacgtcagc aaatggatgt atcagtactt atgttcagaa tattaccata
    25981 tcatcacctc cggttgcaaa tgccggtgct gatgcagcaa tttgtcttgg tcagtctgtg
    26041 acacttactg caagcggagg attaagctat caatggagta atgggcaata taccaatccg
    26101 gttaacgtta gtccgtcaca atccacgacc tatacagtta cagttacaga tgttaatgga
    26161 tgtgttgctg ttgattctgt gcttgtaaac atcaatcagt tgcccgtggc agttgtttcg
    26221 ccaccacagt caatttgttt tggtgataca gctacgctaa ctgcatcagg aggggtaagt
    26281 tattcatgga gtccggtcgg attgacaggt tcagttataa atgtgaaccc taattcatca
    26341 tcagtctata ctgttttggt tactgataat aatggatgta ctgccaatgc acaggtaaat
    26401 gtaacagtaa attcaaaccc tgttgtaaat atacccacaa catttgtttg tacaggaacc
    26461 ttcacaactt taaatgcagg aaatgcaggt tcatcttatt tgtggtcaac cggtgataca
    26521 actcaaacaa ttgatgtcac cgcacccggc aactacgcag ttactgttat aaatagcttt
    26581 ggttgctctt caacagatac tgtattggta acgccaggtg gttcaattac caacaatcaa
    26641 gcaaatattt cgatgtgtca gggattgacg gcaacattgg atgcaggaaa ccctggtaac
    26701 agttttctgt ggtcaactgg tgcagtaaca caaactatta ctgtttcgag cagtggtcaa
    26761 tatgcagtta ccataaccaa tcttaatgga tgtactgcca ctctgattag tacggttcag
    26821 gttaatcctt tgccttcagc tgccataacg attaatgatg catgtataaa tcaagcgatt
    26881 cagttttttg acagcagcag catcagtgca ggaaatattt caaactggca gtggagcttt
    26941 ggtgatagtg cattgtcaaa tgttcaaaac ccaacgcata attttgctaa tgccggaact
    27001 tacaacatca gccttgtagt tacttctgct gcaggttgta tggattctat atcgcaatcc
    27061 atcacaatat ttccaaaacc tgttgtagca tttagcggtg tcaatgcctg tcccggtagt
    27121 gctattcagt tccagaacag ctcttcagtg tcaacaggaa gtattaatgc ttatcattgg
    27181 aactttggcg atggtacaac atcttccgtc ttaacaccaa ctcatacata cacttcttcg
    27241 ggaaattatc aggttgcgct gacagtaaa
//
LOCUS       DAOQNP010000036        26320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_615_length_26320_cov_9.094460, whole genome shotgun sequence.
ACCESSION   DAOQNP010000036 DAOQNP010000000
VERSION     DAOQNP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26320
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_615_length_26320_cov_9.094460"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..2466
                     /locus_tag="PKY09_06545"
     CDS             529..2466
                     /locus_tag="PKY09_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNB12553.1"
                     /translation="MVARHDPLNKITFGGLLVTLGIIYGDIGTSPLYVMNAIIGDRVI
                     SPELVLGGISCIFWTLTLQTTFKYVFLTLRADNKGEGGIFSLYALVRKTKVKWLVFAA
                     ILGGSALLADGLITPAISVSSAVEGIQILHPDLPTIPIIISIIFFLFFIQQFGSNFVG
                     KSFGPIMLLWFSMLFILGFHQLMQNPSVLKAVNPYYAYQLLVNYPEGFWILGAVFLCT
                     TGAEALYSDLGHVGRKNIYITWAMVKICLLINYFGQGANLLKFEGKTIDVNPFYQLMP
                     EWFLLIGIIISTTAAVVASQALISGAFTVVNEAMRLNFGPKLKVVYPTDLRGQVYIPT
                     VNWVLCVGCIGVILFFQHSSNMEGAYGLAIIINMMMTTILLSFYLYLKRYPLPLVMLT
                     FLLFIVIEIAFLVANSIKFEHGGYVTMILAMFLFVTMYVWYAARKIRNQFVDFVKVRD
                     FLEILTNLSKDESIPKYATNLVYLTSANRRDEIESKVMYSLLQKFPKRADIYWFVHVD
                     VLDEPYTMEYKVEEMVNDKVIHIDFRLGFRIEPRINQMFRQVVEELVKNNEVDITSRY
                     TSLKEKNVIGDFRFVVIEKTLSYDNKLTFYEKFVLNAYDFLKMLSLPESKAFGLDTSS
                     VTVENVPLVVKPSGKFKLKRI"
     gene            complement(2463..3668)
                     /gene="hflX"
                     /locus_tag="PKY09_06550"
     CDS             complement(2463..3668)
                     /gene="hflX"
                     /locus_tag="PKY09_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNB12554.1"
                     /translation="MKEKTVFKPLTDVKERAVLVGIILKDQPVEQELEYLDELAFLAE
                     TAGAQTLKSFTQKLDHTDPRTFVGSGKLAEINEFVKANEINLAVFDDELSPSQQRNIE
                     KILQIKVLDRTQLILDIFARRARTAQAQVQVELAQYQYLMPRLTRMWTHLEKQQGGIG
                     NRGPGEKEIETDRRIVRDRLSKLRERLKEIEKQGFNQRKARGNMVRVALVGYTNVGKS
                     TIMNLLSKSDVFAENKLFATLDTTVRKVVLDNIPFLLSDTVGFIRKLPHHLVESFKST
                     LDEVREADILIHVVDISHTNFEAQINVVNQMLQELQSANKPVILVFNKIDAFTYIEKE
                     ADDLTPLTRENMSLDDLRKTWMNKGNVETIFISATEKENITELRSLLLEKVKRIHLVR
                     YPNNKIKND"
     gene            complement(3691..4311)
                     /locus_tag="PKY09_06555"
     CDS             complement(3691..4311)
                     /locus_tag="PKY09_06555"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNB12555.1"
                     /translation="MYTGILHTHTLVVSLFIVLYLIKATLLVLNRHAALDNFVKKTKV
                     AEMIISTLFLLTGIYLAVNTGNKGTWLWVKLFVIACTIPLAVIAFKKKNSSLALISLM
                     ALIYAYGISETKSISMKKADTALNTEGLEGVAAGKVIFESKCQSCHGADGKLGLSGAK
                     DLTQSALSPQEKIAIITNGKKTMAAYKDQLTPEQITAVAAYVQTLK"
     gene            complement(4329..4416)
                     /locus_tag="PKY09_06560"
     tRNA            complement(4329..4416)
                     /locus_tag="PKY09_06560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4380..4382),aa:Ser,seq:gga)
     gene            complement(4609..5631)
                     /locus_tag="PKY09_06565"
     CDS             complement(4609..5631)
                     /locus_tag="PKY09_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNB12556.1"
                     /translation="MKTDKKNLLVIYTGGTIGMIHDEETGSLKPFNFGRLSTVVPELK
                     KLPCKIDFHSFDPLIDSSDMQPERWIQIAEVIEENYGKYDGFIVLHGTDTMAYTASAL
                     SYMFQNLNKPVILTGSQLPIGEIRTDAKSNLITAAEIASGNVVVPEVCICFNNQLFRG
                     NRAEKFTSSKFDAFQSINYPSLAEAGTEINYNHDAIREVNNNALKVKAHFNNSVMIFK
                     LFPGVNEKVLSAILKSGALKGLIIETYGSGNAPKDEKLLKQLSDAVKNQMVIVNVSQC
                     SGGVVQMEKYENGLALKRAGVVSGYDMTTEAAITKLMFLLGSEKDFQEIKVNMQLDLA
                     GELSRE"
     gene            complement(5631..6395)
                     /locus_tag="PKY09_06570"
     CDS             complement(5631..6395)
                     /locus_tag="PKY09_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNB12557.1"
                     /translation="MIFTDTHTHLYASEFDADRNELMQQAIDAGVSRFFLPNVDKSSI
                     NAMLELHNQFPGNCFPMMGLHPCSVKENYLDELALVESELSKGNYFGVGEIGLDFYWD
                     KGFIKKQEEAFLKQIAWACELNLPVSIHSRSATARAIELIQLSGLNVKGVFHCFSGSI
                     EEAKAITDMGMFLGIGGVITYKNSGLDKIVSQVALKHIVLETDSPYLPPVPHRGKRNI
                     PEYLLLTAQKLAVIKNCSLAEVAEQTTNNSKVIFKQ"
     gene            6482..7477
                     /locus_tag="PKY09_06575"
     CDS             6482..7477
                     /locus_tag="PKY09_06575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNB12558.1"
                     /translation="MSRVLVIRFSSIGDIVLTTPVLRCIKKQLPNTEVHFLTKRDFSD
                     VLANNPYIDNTFYVDNNLPEIIRQLKMVGYDYIVDLHHNMRSWKVKRALRAKKFSFNK
                     LNIEKWIYVNFKINKLPHKHIVDRYFDAINPMGVVNDGQGLDYFINPLDEVDINTFLP
                     ASFSRGYVAFVVGAKHATKCLPLEKMKEIISGLKYQVVLLGGFEDKQKGATLQQLFPE
                     KVFNACGTTSIGQSASLIKQSKAVITHDTGLMHIAAALRKKIVSVWGNTVPEFGMYPY
                     LPLNSVPFSINEVKNLKCRPCSKIGYNTCPKKHFRCMNDMDVNEIIKNTDYYFQS"
     gene            7582..8454
                     /locus_tag="PKY09_06580"
     CDS             7582..8454
                     /locus_tag="PKY09_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNB12559.1"
                     /translation="MSEDNKLARKALRSSNISTIISISLVLFLIGSLGLLLINAKQLS
                     DYLKENVILTVMMKQEASDADIQNLKKQLEGNAFIKEVSYISSEQAAEQLKQELGEDF
                     NTFLGFNPLLPLIEIKLKPDYTEENNISQLKDFISKNSIVKEVYFQESLLDAINKNIR
                     NISLIILGFSAVLFFIAGALINNTIRLALYAKRMLIKSMKLVGATRNFIRKPFIVRGI
                     LQGFIGAVIANIMILMVFYYAKKQIPEIIAFENPVNLSIVLGIVVFLGIFISGFSTYF
                     AVNKYLRQRTEDIF"
     gene            8533..8745
                     /locus_tag="PKY09_06585"
     CDS             8533..8745
                     /locus_tag="PKY09_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNB12560.1"
                     /translation="MAFGKINYMLMIGGIVFILLGYMLMSGGGSADPKVFNPAIFDTQ
                     RLTVAPIMVVLGYALEVLAIVVNAKD"
     gene            8766..9566
                     /locus_tag="PKY09_06590"
     CDS             8766..9566
                     /locus_tag="PKY09_06590"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNB12561.1"
                     /translation="MNTLQAIILAIIEGITEFLPVSSTGHMIIGSSVMGIAELPITKT
                     FTVAIQFGAILSVVFLYWRRFLVIDFNFYKKLLIAFIPAAVLGKLLDDYIDALLENVL
                     VVAAMLLVGGIIFILLDKWFQKTEERGSEELSKDKSAFYIGLFQCIAMIPGVSRSAAT
                     IIGGLAQGLNKKAAAEFSFFLAVPTMFAATLYKMYGFYKLNGGFTTEEIKLFAVGNVV
                     AFIVAAIAIKSFINYLTKHGFKIFGWYRIIIGAAILLLHFAGIELKML"
     gene            9572..10282
                     /gene="truB"
                     /locus_tag="PKY09_06595"
     CDS             9572..10282
                     /gene="truB"
                     /locus_tag="PKY09_06595"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNB12562.1"
                     /translation="MPDFLLGETLLVDKPLEWTSFDVVNKLRGTLQHLKLSDGSRPYK
                     KIKVGHAGTLDPLATGLLIVCTGKATKSIETLMGLEKEYTGTFTLGATTPSYDAETPV
                     NKTFDIAHITTEQIHQAAQTFIGETEQLPPAHSAVIIQGERAYEKARRGEEVKVTPRK
                     INIHDFEITEIAMPVVSFRVVCSKGTYIRSLANDFGKALNSGSYLSSLIRTRIGDFKL
                     SAAHTVDHYVDAIRNLHK"
     gene            complement(10536..12398)
                     /locus_tag="PKY09_06600"
     CDS             complement(10536..12398)
                     /locus_tag="PKY09_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12563.1"
                     /translation="MKKLSLLILILLIAQLANAQIPFQKSFGGTNTSWGNSIQQTNDN
                     GYISVGVTDAFGVGAFDVFAIKTDSSGNPIWKKAIGETSSDFANDVQQTSDSGFIVCG
                     YSISSFSGGFAGENIYLIKLNSNGSILWSKIIGGVGNERANSVRQTSDGGFIIAGTTN
                     SWGAGGNDFYLVKTDGNGNISWTKTFGGALDDVANSVKQTSDGGYIVAGETNSFGAGD
                     IDYYLIKTDALGTLTWSKTFGGGGSDWLYSVVQASDDGYVMGGYSISFLGAGEADAVV
                     IKTDITGSILWSKTYGEQYGDVASSIVRTNDNGYALAGGQSSFPFSMVDKGLLFKIDS
                     VGNFIWANAFINSNEVQRGICKTTDGGYALTGEWLPVSGIDLILIKTNANGTVGCNEL
                     STTLISTNIFPQISNPATLSSNGGAALTVTSLSSNGGIVTNPCSCSSYFTLYADIIPH
                     NWIAVNQATGQQPITYSWNWGDGSANSSGSTPSHTYNTAGYYNICLSITDFAGCTSIY
                     CDSSTYLYKSNSNGTMVNVNVILPTGINIPEPNENTISFFPNPTSGVFIIFFPENFRK
                     GKIEIYNVMGEKIYIDVVDGESKKEINFKNISAGIYFVKVFDVEKSFCKKLIVE"
     gene            12566..12877
                     /locus_tag="PKY09_06605"
     CDS             12566..12877
                     /locus_tag="PKY09_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB12564.1"
                     /translation="MQYIQPQNRAQVTFTSLEDTVAPNNAVRLIDAFVEHLDLAQLKF
                     NVSVLNKEGRPAFESKVFLKLYFYGYLNGIRSSRRLEKETQRNVELHLHSHSSSLKSS
                     S"
     gene            complement(13001..17431)
                     /locus_tag="PKY09_06610"
     CDS             complement(13001..17431)
                     /locus_tag="PKY09_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12565.1"
                     /translation="MKQLLLFLIVGYALLANECSAQCTGGTLASNLTPGVTWSTTGTT
                     AVAAGKYYTFTATAGFVYYFSFCAADGGNATWDTQITILNNAGTYVGAGGFSDDNCGL
                     QSYLAWTCVTTGTYRVLITKYSCTTGATGATMSYKYSAPLSCPSNLGTGNVAIASLPY
                     SSGAGTTCGAGNDLTSVNTNPCGSTFYLDGEDKVFIFTPASTGTVTITLNAPSASYTA
                     LNLYNGCPLTGQPGTCVANSQSSTGSKTLTVCLQAGTTYYLILDTWPAPSCVPFNDLT
                     ISAPVPAGACATGNGTVNVASLPYTSNSRTTCGKTDDFTTTNTIACGSTFYNGAEDEV
                     FIFTPSTSGQTTISLTSASSWVGITLFDGCPLVTSCSGTAGTCVAFAQSSAGSQSMCV
                     NVIAGHTYYLVVDQFPSPYCIPSYNISISAPVGMLPGAICSNAVNISGLPFSAANETT
                     ACMGNDYTNASIGSCGTLYESGEDKVYRYVASGPECISITLSQASDNLIGYQVYSGCP
                     GNAGTNCIGNNSGALSGTLNGSVVLPAAGTYFIIVDTWAPPTSVTYDISIQSFGSGAA
                     NDLPCNATPLPIGVYLNGSNNCSGLAGEPSNPSCWSAGSRNTVWHSFVAPPSGQVTIR
                     TVPGSLRNSQIALYQGTCGASMTLLACNDDAPSCGTTINYMSQINYSGLTAGTTYYVV
                     VDGYNNETGSYGIIIVSGPPANIPPVFGQECSVPNPVCNQIISIGDPGYQAFGSSCDF
                     PGSGSNCLLSAERGSAWYEINIQNAGVLAFNIIPNDYVAGLPGNETDYDFAIWKMKDG
                     PTNNTVNTCATLASGAAPIRCNYSYLGVTGLNSTVTNQAPAPYTPDYNAAYDAQINVA
                     AGDVYQLVVSNFSNSTSGFTMNFLSSPVNFTPNPGFVVWSGGVDNDWFKAPNWGGCQI
                     PNCTVDAIIPPSSANMPIINAAGASCKSITINPSASLSINAGFSLQVCQDYNNNGTLN
                     AANGSNVYFNNASVNQSLNGNMVGASAFANVVVSKTGGIVTLGQNADMKGNFIITSST
                     SNFNANAKTHRVGGDFLNYGTYTPGIGGTLELYGTAAQNYLNNGVLNNLSMNHSGTGV
                     TLLSNATLGNNGMLSLTNGKIITNLFEVTVNNRNSGAVTPGNMNSFIQGNLRRYLNTQ
                     GSYDFPVGHATAGYQRMNINFNVPGSPTTIDNLLSFFNTYSVIPAGIGATECSVVYSQ
                     PALDNGYWSFTASNSPLSGTYDATAYNLNYSNPFMGWTIMRNHGAGWNLFNGICINSP
                     VTAVNRVAMNGFGTGTELFGITQANNPLPVELIAFNASPLVNSIMVAWSTASEINSDY
                     FELQRSTNGIEFTTIANITSQGNSNVVSDYKFEDKDVVKNTIYFYRLKQVDKNGSYVY
                     TEIRNAKIDDDSNIFVITPNPYKNETNFVIFLNSNADVSIEVINVIGEVVSTQINEKL
                     EKGMHQYTFSAKKLGLPAGIYNVKLNINGVITSKNMIELE"
     gene            complement(17471..17827)
                     /locus_tag="PKY09_06615"
     CDS             complement(17471..17827)
                     /locus_tag="PKY09_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12566.1"
                     /translation="MIKKLLITFLLLFNGLLFAGDDGNQELILRINELSNQKRHAQID
                     NILSTTEGIVSKEYCYSLNCVMLIINSTEYNSRAILLNKLKNEGFTFTERNGVTISRV
                     KQACKDSNNSDMIKNQ"
     gene            complement(18083..19702)
                     /locus_tag="PKY09_06620"
     CDS             complement(18083..19702)
                     /locus_tag="PKY09_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12567.1"
                     /translation="MKKFIKYLFALTAQLLIQEESNVVYAQNLVKNPSFENYVQCPVG
                     AGLFNGYVIDWFGFNPGSPSFFHSCATVPINSYGFQYANTGGGYACILAYNWNYDPDK
                     RNYIEGDFIYALKKDSIYCINYFVNLCNNSYGGIKNVDAAISDTLINWNNGAGNFLIN
                     IYPQIKSSTIITDSVNWTLISGLYKAKGNENHITIGNFELYANTSYQSFYPVGTPISI
                     SYYIDDVSVTPTNLTAPNLGNDTIICKNTLPYSLYAPVGYDSYQWSNGGTSSIIAVND
                     SGMYWVKCILTGCGEISDTVHISFKNIHKLNLGNDTIVCVGSSVNLTAQPNFISYLWN
                     TSDTSQNILVNDSGLYIVQATDVCGMQTDSVHVTLDSLPSITIDIGIDTTICNRGTNV
                     PFLLSSNVTLPNYNWSNGAITSQISVTKKGWYWLQSKYNCGTIESDSIFIDECPPDTL
                     FGLYLPNSFTPNDDGLNDFFTPQFYNVTITNFKIFNRWGNVVYEDSNNFSWDGKFESK
                     DCPLGVYVYKISYRDLKNNYIEKLGRITLLR"
     gene            complement(19751..22033)
                     /locus_tag="PKY09_06625"
     CDS             complement(19751..22033)
                     /locus_tag="PKY09_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12568.1"
                     /translation="MKKLIINVTIVALLMAVTNSFAQMIYWTTPPYRINTSTSIPTSS
                     ALPGGGGAYSVANGAYDPSGNLLFFVRDYGIYGPTGSNVGTLAPYNSQVCAEEYSILC
                     SEVAIVPIPGTCNQFYVIYSMDNPVGYSPVLYVKVDCSGATPVVTYNGSVYVNCPPIF
                     VGYKNQAFWVSGHGGADHTAIAVSKVYTGSGSTAKRFLFSVSNNGIVRSDITNTGISA
                     GTTVATYATLGLPAVDAFECEVSWGTNLLAWSNINGTVHVIGIVSTTGAYQPSTLQNY
                     NVTAARGIEFTNATTNPKLYVSGSGGLTQILTATQVQSSILTPGYNFTNTYLEYGKNN
                     RIYGISPTFSGPTLTATTFAGINTSSNTISAYNPGFVDSRFTYGYLGQYTCFTLPNQI
                     DGESYAVFSGLPNVTIANFTLNGSSASGNCETTSMPVYYNCNPLVFNATYNNGTPTQY
                     KFDIQAIDGGCQLTTGGVNYHGLWTAGQPTANLNLFTLTGTFTGTVQVKFSIQDACGN
                     VSTQIKNINILGPPPAANSVFKLYNGNGGSAFANGTTCTAPYALCQTSDGGIYANFST
                     GTVCYYNVKLEKLTGACPNGFTNLGTTPNYNVSACNPVNITPKTMNAIGSDMGLGLGY
                     FDIAGNLNTTYRITLTVGNACSSNSAIGYFNPSNASCRMANPNSNTESQNNVSGISVY
                     PNPASDNVTVEFSASADDYYEIEITDMLGKQVMLLMPLTKISKGVFKKSFDISKLNNG
                     IYTYRIKSSTVNQSGRISKN"
     gene            22284..22925
                     /locus_tag="PKY09_06630"
     CDS             22284..22925
                     /locus_tag="PKY09_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12569.1"
                     /translation="MFHRGKTDFDVLILSKDTLFVEVMVEKLKPLFSNISAKHIQSAD
                     LIGVFQFRSLFIHQSSVLDFYQNNNKCKNASNAFSIIFFLWEKPECFQYYPLKCNFKI
                     IDLKIPAAKILKEVSQILLPVKSNEDAIFEQLGVLNKQLSNILQTALFTDDELDIIYK
                     LKKFSDNKTLAQKCNISIATLKRRLKIIAEKCDNAKSKSEIINFLYENYVIKP"
     gene            complement(23176..23886)
                     /gene="pyrH"
                     /locus_tag="PKY09_06635"
     CDS             complement(23176..23886)
                     /gene="pyrH"
                     /locus_tag="PKY09_06635"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015806019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNB12570.1"
                     /translation="MKYKRILLKLSGEALMGEKNFGIDNNRLTQYAEEIKTIVDKGVQ
                     VAIVIGGGNIFRGLQAAEGGMDRVQGDYMGMLATVINSMALQGALEHKGINTRLQSAI
                     TMDAICEPFIRRRAVRHLEKNRVVIFGAGTGNPYFTTDTAASLRAIEVEADVILKGTR
                     VDGIYTSDPEKNSDAVRFEHLTFDDVYEKDLKVMDMTAFTLCHENKLPIIVFDMNTPG
                     NLLRLVEGNHVGTLVDVE"
     gene            complement(23920..24825)
                     /gene="deoC"
                     /locus_tag="PKY09_06640"
     CDS             complement(23920..24825)
                     /gene="deoC"
                     /locus_tag="PKY09_06640"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNB12571.1"
                     /translation="MAKADLRFLNHLPPVDQVGVEERVARLNKRSIKNESKMEALKLA
                     ISMMDLTTLDAKDTPGKVRQLCAKAIRPHEMEGIPSAAAVCVYANLVSVAKQALEGTK
                     VKVAAVSTAFPSGMSNRKFKLDETRYAVAEGADEIDMVISRGEFLRGEYNFVFDEIAA
                     VKEACGKAHLKVILETGELSTLDNVRKASELSIAAGADFIKTSTGKVQPAATLPVTLV
                     MLECIRDYYYRTGIKIGMKPAGGIATAKLALQYLVMVRETLGQEWLNADMFRFGASSL
                     ANDLLMQILKQITGAYQSADYFSKD"
     gene            24965..26311
                     /gene="purB"
                     /locus_tag="PKY09_06645"
     CDS             24965..26311
                     /gene="purB"
                     /locus_tag="PKY09_06645"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNB12572.1"
                     /translation="MSDALFAVSPVDGRYAAVCSPLREYFSEYALIRERVKVEIKYLI
                     ALAQLQLPQLQIDSGKFDALRSIYRQFTPADAEQIKQTEKTTNHDVKAVEYFVKEKLE
                     ALGLHKAKEFVHFGLTSQDINNTATPMLVRHGIAEVMLPALTDVLNKIESQAQDWNNI
                     SLLAHTHGQPASPTRLGKEMKVFSERLRAQLEMLKSIPHSAKFGGATGNFNAHAVAYP
                     AVDWISFADDFVNSLGLTRTTHTTQIEPYDNLAALFDNLKRINNILLDLCRDVWTYIS
                     MNYFSQTVKTGEIGSSAMPHKVNPIDFENAEGNLGIANALFEHLSAKLPVSRLQRDLT
                     DSTVLRNTGVPFAHTLIALKSLLKGLNKILPNTQVISADLNSHAVVIAEAIQTILRRE
                     GYPNPYEALKDLTRGKENITLQDIHQFINTLQVSDAVKAELLKITPENYTGVNLSD"
BASE COUNT         8086 a         5241 c         4698 g         8295 t
ORIGIN      
        1 ataccatgtt tacaaagttt ccttcatggc tcaaataccg accttctgaa aaaacacgtt
       61 aaacaaaagc cacttgtatg agcaaaaaga aaacaaactg tctgaagttg tcggtatgtc
      121 cgcctgtggc ggattgcgac aacaagtttt tgttttcgtg cgtagctggc ttttgtagtg
      181 gtttttcaga cagtcttgat ttttaggttc ttttgtatca agacaaagaa tgaaggaaga
      241 ttttccaagt tacctattcg tggagtacac tgcaatacat aacctcgcca aaaattataa
      301 atgttaatta tcaatgtata ataacaaaca attaccaaag cgtagttaaa aaattgctgg
      361 tatataagca ctaaaaaaga aatgcccaac tattccatta tcatcatagc attaatcact
      421 ctgctgtcaa atatgaatta ttaaaattca attacaattg aggttttttt ttaaaaactt
      481 tcttcaacaa acctttcgaa atcatacctt tgccatccta ttaaaaatat ggttgcacgc
      541 cacgatccgt taaacaaaat aacctttgga ggtcttttag tcaccttagg aattatttat
      601 ggtgacatag gtacatcacc tctttatgtg atgaatgcta ttattggcga cagagtaata
      661 agtccggagt tagttcttgg tggaatcagt tgtatattct ggactttaac gcttcaaact
      721 acttttaaat atgttttcct cacattacgt gccgataaca aaggtgaggg tggaattttt
      781 tcgctctatg ctttggttag aaaaaccaag gtaaaatggt tagtgttcgc tgcaattctt
      841 ggtggcagtg ccttgcttgc cgatggttta attaccccag ccatttctgt ttcttctgct
      901 gtggaaggta ttcagattct tcaccccgac ctgcctacta ttcctattat catctctatt
      961 attttctttt tgttttttat tcaacagttt ggaagcaact ttgttggaaa atcatttggt
     1021 ccgattatgc tgttatggtt ttcgatgttg tttattttag gctttcatca actaatgcaa
     1081 aatccatctg ttcttaaagc tgttaatcct tattatgcct atcaactttt agtcaattat
     1141 cctgaaggtt tttggatttt aggtgctgtt tttctttgta caacaggagc cgaggcattg
     1201 tattccgatt tgggacacgt aggccgaaaa aatatttata tcacttgggc aatggtaaag
     1261 atttgtctct tgataaacta cttcggtcaa ggggccaact tgttaaagtt tgaaggaaaa
     1321 actattgatg tgaacccatt ttatcagtta atgccggaat ggtttctgtt gattggtatc
     1381 attatttcca ccacggcagc agttgttgca agtcaggcgc tcatcagtgg ggcatttaca
     1441 gtagtgaacg aagccatgcg attgaatttt ggtccaaaac taaaagttgt ttatccaacc
     1501 gacctcagag gacaggtgta tattccaacc gtaaattggg ttttatgtgt cggttgtatc
     1561 ggtgtgattc tgttttttca acactcttcc aatatggaag gtgcttatgg attagcaatt
     1621 atcattaaca tgatgatgac tactatttta ttatccttct acctctacct caaacgttat
     1681 cctttaccgt tggtaatgct cactttttta ttatttattg taatagaaat agccttttta
     1741 gtagccaact caataaaatt tgaacatggt ggatatgtta ccatgattct tgccatgttt
     1801 ttattcgtaa ccatgtatgt ttggtatgca gcaagaaaaa tcagaaacca gtttgtggat
     1861 tttgtcaagg ttagagactt tttggaaata ttaaccaacc tgagtaaaga cgaatctatt
     1921 ccaaaatatg ccaccaacct ggtatatctg accagtgcca accgcagaga tgaaatagaa
     1981 tcgaaagtga tgtattctct cttacaaaag ttcccaaaac gtgccgatat ttattggttt
     2041 gtacacgttg atgtgcttga tgaaccctac accatggaat ataaggtaga agaaatggtg
     2101 aatgataaag taatacacat tgacttccgt cttggattcc ggattgagcc ccgtatcaat
     2161 cagatgtttc ggcaggttgt tgaggagctt gtgaaaaata atgaagttga catcacgagt
     2221 cgttacactt cacttaaaga aaagaatgta atcggtgatt tccgttttgt tgttattgag
     2281 aaaacgcttt cttacgataa caaactcacc ttttacgaga aatttgtgct taacgcttat
     2341 gactttctaa aaatgctgag cttaccggaa tcaaaagcat ttggacttga caccagttct
     2401 gttacagtag aaaatgttcc tttggtggtt aagccatcag ggaaatttaa actgaaacga
     2461 atttaatcgt ttttgatttt gttattggga taacgaacca agtgaatacg ctttactttt
     2521 tctagcaaca agctacgcag ttcggtaata ttttcttttt ctgtagcaga aataaaaatt
     2581 gtttctacat ttcctttatt catccaggtc tttcgtaaat cgtctaacga catattttcg
     2641 cgagtcaggg gtgtaaggtc atcagcttct ttctcaatat aagtaaatgc atcaatttta
     2701 ttaaaaacaa gaattacagg tttgtttgca gattgaagct cctgtaacat ctgattcacc
     2761 acatttatct gtgcttcaaa attggtatga gaaatatcta ctacatgtat taaaatatcg
     2821 gcttcgcgta cttcgtcaag tgttgactta aatgattcaa ctaaatgatg gggcagcttt
     2881 ctgataaatc ctacggtgtc gctcagtaaa aatggaatat tatcaagcac tacttttctg
     2941 actgtagtat ctaatgttgc aaagagtttg ttttctgcaa acacatcaga cttgctaagc
     3001 agattcatga ttgttgactt gcccacattg gtataaccta ccaacgccac acgcaccata
     3061 ttgcctcttg ctttacgctg attaaaacct tgcttttcta tttctttcaa acgttcacgc
     3121 agtttactca gcctgtcgcg tacaatacgt ctgtctgttt caatttcttt ttcaccgggg
     3181 ccgcggttac ctattcctcc ctgctgcttt tccaggtgtg tccacatgcg tgtgaggcgt
     3241 ggcatcaggt attggtattg tgctaattct acctgcacct gtgcctgtgc tgtacgtgct
     3301 cttcgtgcaa aaatatcaag gatgagttgt gttctgtcta acacttttat ctgtaatatt
     3361 ttttcaatgt tgcgttgctg cgaaggcgac aactcatcat cgaaaactgc aaggtttatt
     3421 tcattcgctt tgacaaactc attgatctcg gccagttttc ctgaacccac aaaggtgcgc
     3481 gggtcggtat gatctaactt ctgtgtaaac gactttaatg tttgtgctcc ggcagtttct
     3541 gcaagaaaag ctaactcatc aagatactcc aactcctgtt ctacaggctg atcttttaat
     3601 attataccaa ctaaaacggc acgctcctta acatcagtaa gaggcttaaa aacggttttt
     3661 tctttcatca ataaattaaa ctgaaataaa ttatttcagt gtctgaacat acgctgcaac
     3721 ggctgtaatt tgttccggtg ttaattgatc tttataggca gccattgttt ttttaccatt
     3781 ggtaatgatg gcaatttttt cttgaggaga taaagcagat tgggtaagat cctttgcacc
     3841 actcaaacct aattttccat cagcaccatg acaagactgg catttgcttt caaagataac
     3901 cttacctgca gcaacacctt caagaccttc tgtatttaaa gcagtatctg cttttttcat
     3961 ggatatggat ttggtttcag aaatgccata agcataaatc aatgccatga gtgaaatcaa
     4021 tgccaatgag ctgtttttct ttttaaaggc aattactgca agtggaatgg tgcaggcaat
     4081 aacaaacaat ttaacccaca accatgttcc cttgttgcct gtgttgacag ccagataaat
     4141 tccggttaac aaaaacaatg tgctgataat catttctgca accttggttt tcttaacaaa
     4201 gttgtctaat gcagcatgcc tgttcaacac taataaggtt gctttaatga ggtataaaac
     4261 aataaaaagt gaaactacaa gtgtgtgtgt gtgcaaaatt cctgtgtaca taatgataaa
     4321 tgtttttgcg gagagagagg gattcgaacc ctcgttacgc ttttggcgta aacacacttt
     4381 ccaggcgtgc tccttcaacc actcggacac ctctccttta tgagcctgca aagctaacaa
     4441 attaacatga tggctaaaga aaaagcctgc cgaaaaaaaa tacgacaggc tgagaaccta
     4501 aaaccaaacc tatcttttga ccgttgtcaa cagattgatt aaaaccgaat caaaagtagg
     4561 ttgacaaaca gaataactgc gccaatgcgt agtttgttat caacacattt attcacgact
     4621 aagttctcca gccaaatcaa gttgcatgtt cacttttatc tcttgaaaat ccttttcgct
     4681 acccaataaa aacatcagct tggtaatggc agcttctgta gtcatatcat agccactgac
     4741 cacacctgca cgctttaatg caagtccatt ctcatacttt tccatctgca caacgcctcc
     4801 gctacactgc gaaacattta caattaccat ttgatttttt acagcatcac ttagttgctt
     4861 aagcaacttt tcatccttag gtgcattgcc cgagccatac gtttctataa tcaatccctt
     4921 taatgcacct gactttaata ttgctgacaa aactttctca tttactcccg gaaacaattt
     4981 aaaaatcatc accgaattat tgaagtgtgc ttttactttc aatgcattgt tgttaacctc
     5041 acgaatagca tcgtggttgt aattaatttc agtgcctgcc tctgccaaag acggatagtt
     5101 tatagattga aaggcatcaa attttgaaga agtaaatttc tctgctctgt tacctctgaa
     5161 caactgattg ttaaaacaaa tacaaacttc aggaaccaca acatttccag aggcaatttc
     5221 agcagcggta ataagattgg attttgcatc ggttctgatt tcaccgattg gcaattgcga
     5281 tccggtgagt attacaggtt tgttcagatt ctgaaacata tagctcaatg ccgaagcagt
     5341 atatgccatg gtatcggttc catgcaacac aataaatcca tcatacttac cataattctc
     5401 ttcaatgact tctgcaattt gtatccatcg ttcaggttgc atatcggagc tgtcaatcag
     5461 cgggtcgaat gaatgaaagt ctattttaca gggaagcttc ttcaactcag gtacaacggt
     5521 actgagcctg ccgaagttaa agggctttaa tgatcctgtc tcttcatcat gtatcatacc
     5581 tatggttcca ccggtataaa ttaccagaag gttcttcttg tctgtcttta tcattgttta
     5641 aaaataactt tagaattatt tgtagtctgc tcagcaactt ctgccaatga acaatttttt
     5701 atgaccgcta atttctgtgc tgtcagcaat aaatattccg gaatatttct tttacctcga
     5761 tgtggcaccg gtggcaaata gggtgaatca gtctctaata caatatgctt taatgctacc
     5821 tgactaacaa ttttatcaag ccctgaattc ttgtaagtaa ttacacctcc gattcccaaa
     5881 aacataccca tgtcagttat tgcctttgcc tcctctatgc ttccactgaa acaatggaaa
     5941 acacccttta cattaagccc tgataactgt ataagttcaa tggcgcgagc agttgctgag
     6001 cgcgaatgaa tgcttacggg aagattcaat tcacatgccc atgcaatctg ttttaaaaaa
     6061 gcttcttcct gttttttgat aaaaccttta tcccagtaaa agtcaagacc aatttctcca
     6121 acaccaaaat aatttccttt tgacaattca gactcaacta aagccaattc atccaaataa
     6181 ttttctttga ctgaacaggg atgcaacccc atcataggga aacaatttcc gggaaactga
     6241 ttatgtagtt caagcatggc gttaatgctt gacttatcaa cattgggaag aaaaaaacga
     6301 cttacaccgg catcaatggc ctgttgcata agttcattcc tgtctgcatc aaactcactg
     6361 gcatagagat gtgtgtgtgt atctgtaaaa atcatgatgc aaagtaaatc gaatttaagt
     6421 atttaaacca atgcttacct ttgcaaacaa tggcattaga catcttttcg aaaaccacag
     6481 tatgtcccga gttttagtca tccgttttag ctctattggt gatattgttt taacaacacc
     6541 tgttttgcgt tgtataaaaa aacaattgcc caacacagaa gtgcattttc ttaccaagcg
     6601 ggattttagt gatgtactgg caaacaaccc ttacattgat aatacttttt atgttgataa
     6661 caaccttccg gaaataatca gacagctaaa aatggttggc tatgattata ttgttgactt
     6721 acatcacaac atgcgttcat ggaaagtaaa aagggcatta agagcaaaaa aattcagctt
     6781 taacaaactc aatattgaaa agtggattta tgtcaatttc aaaataaaca agttgccaca
     6841 taaacatatt gttgaccgct attttgatgc catcaatcca atgggtgtgg taaacgatgg
     6901 gcaggggctt gactatttca tcaacccact tgacgaagtt gatatcaaca cttttttacc
     6961 tgcatcattt agtcggggtt atgtagcttt tgttgttggt gccaagcatg ctaccaaatg
     7021 cctgccgctt gaaaaaatga aagaaatcat cagcggactg aaatatcagg ttgttttact
     7081 tggtggattt gaagacaaac aaaaaggagc aacacttcag caattatttc cggaaaaagt
     7141 ttttaatgcc tgcggaacaa caagtattgg tcagtcggca tcactcatca aacaaagtaa
     7201 ggcagtcatc acacatgaca ccggactgat gcatattgct gcagccttgc gtaaaaaaat
     7261 tgtttccgtt tggggcaata ccgtccctga attcggtatg tatccctatc tgcctttaaa
     7321 cagtgtacct ttcagcatca atgaagtgaa gaatttaaaa tgcagaccat gttctaaaat
     7381 aggatataat acctgtccta aaaagcattt ccgttgcatg aatgacatgg atgttaatga
     7441 aataataaaa aacaccgact attattttca aagttaatac gttgccacag gctttcacca
     7501 ctatcaaaag tttaataacc ttgttgaata gtttctgttt aaggtcttta caacagtgct
     7561 atacctatta aatttgctgc catgagtgaa gataataaac tggcacgtaa ggcacttcgc
     7621 agttcaaaca tttctaccat aattagcatt tcacttgtac tgtttctgat aggttcgctg
     7681 ggattattgc tcatcaatgc caaacaactt tctgattact taaaagaaaa tgtaatctta
     7741 acagtaatga tgaagcagga agcctctgat gccgacattc agaatcttaa aaaacaatta
     7801 gaaggtaatg cttttattaa agaagtatcg tacatcagca gcgaacaggc agcagaacag
     7861 ttaaaacaag aactgggcga agattttaat actttcttag gatttaatcc attgctaccc
     7921 ctgatagaaa ttaaacttaa acctgactat actgaagaaa ataatatttc acagctaaaa
     7981 gattttattt ctaaaaacag catagttaaa gaagtttatt ttcaggaatc gttgctcgat
     8041 gccatcaata aaaatatcag aaatatcagt ctcatcatac ttggattcag tgctgtgctg
     8101 ttttttattg caggtgcatt aatcaataac actatcaggc ttgcactcta tgcaaaaaga
     8161 atgctcatta aaagtatgaa gttggtaggg gctacaagaa attttatcag aaaaccattt
     8221 attgtaagag gaattttaca gggtttcatt ggtgctgtca ttgctaatat catgatttta
     8281 atggtgtttt attatgccaa aaagcagatt cctgaaatca tagcatttga aaatccggtg
     8341 aatttgagca tcgttttagg tattgtggtg tttttaggta tttttatttc aggattcagc
     8401 acctattttg cagtaaataa atatcttcgc caacgaactg aagacatctt ttaataattc
     8461 aaattcatgg caaaggaaaa aaccataacc aaaaatacca cagcaaaacc tgcatcgaaa
     8521 ccggaaatac aattggcttt cgggaaaata aattacatgc ttatgattgg cggcattgtt
     8581 tttatactgc ttggctacat gcttatgagt ggtggcggtt ctgctgatcc gaaagttttt
     8641 aatcctgcca tttttgatac acaacgatta acagtagctc caataatggt agtgcttggc
     8701 tatgcccttg aagtattagc cattgttgtg aacgcaaaag attaaacaca cttccaaata
     8761 ttttaatgaa tacactgcag gctatcatct tggccattat tgaaggtatc acagagtttc
     8821 tgccggtatc ttctaccgga cacatgatta tcggctcgtc cgtaatgggc attgccgaac
     8881 tacctattac caaaactttt accgtggcca ttcagtttgg tgctatttta tctgtggtat
     8941 ttttatactg gcgcaggttt ttagtaattg attttaattt ttacaaaaaa ttactgattg
     9001 catttattcc tgcagctgtt ttgggtaagc tgcttgacga ttacattgat gccttgctcg
     9061 aaaatgtact tgttgttgca gccatgctgc ttgttggcgg aattattttt atacttctgg
     9121 acaaatggtt tcaaaaaacg gaagaacgcg gatccgaaga actctcaaaa gataaatctg
     9181 ccttttacat aggattgttt caatgtattg ccatgattcc gggagtatcg cgttcggcag
     9241 ccaccatcat tggtggattg gctcagggac tcaacaaaaa agcggctgca gaattttcgt
     9301 tctttctggc tgtgcccacc atgtttgcag caacactata caaaatgtat ggcttttata
     9361 aattgaacgg aggtttcact acagaagaaa taaaattatt tgctgtaggc aatgtagtag
     9421 cttttattgt tgctgccatt gctatcaaat catttattaa ttacctcacc aaacatggtt
     9481 ttaaaatttt tggctggtac agaataatta ttggtgctgc aattttactg ttgcactttg
     9541 caggcattga acttaaaatg ctttagtcgc aatgcctgat tttttactgg gcgaaacact
     9601 tttggttgac aaaccattgg aatggacttc gtttgatgtg gtgaacaaac taagaggcac
     9661 cttgcaacat ttgaaactta gtgacggaag ccgaccatac aaaaaaataa aagttggaca
     9721 tgctggaact ttagacccac ttgcaacggg tttacttatt gtatgcaccg gtaaggctac
     9781 caaaagcatt gaaacattaa tggggctcga aaaagaatat acaggaactt ttaccttggg
     9841 cgctaccaca ccttcttatg atgcagaaac accggtaaac aaaacctttg atatagcaca
     9901 catcaccact gaacaaattc atcaggcagc gcaaactttt attggagaaa cagagcaact
     9961 gccaccggca cattctgcag taatcattca gggcgagcgt gcctatgaaa aagcacgaag
    10021 aggcgaagaa gtaaaagtaa cccctcgaaa aataaatata catgactttg agattacaga
    10081 aatagcaatg cctgttgttt catttcgtgt tgtttgcagc aaaggaactt acattcgctc
    10141 tttagccaac gattttggca aagcacttaa ttcgggttct tatttaagtt cactcatccg
    10201 tacacgaata ggagatttta aacttagcgc agcacacaca gttgatcatt atgttgatgc
    10261 cataagaaac ttgcataaat aattgtggaa ttccaagaaa aaattaaaat tagtttcttc
    10321 atttcagttt caaattagca ctatagttta ttcgaagcgg aaagcatatc cgctaacaga
    10381 tgcgggatta aagaagtaaa aaaagtaggt atctcaagtg gcgaggcgaa gctcgccatc
    10441 gcgaggtttt actcgtgacc cttctttttt ttatttcttt taatctttat gttatcggat
    10501 gtgctactgc ctgtttgaaa ttctaccatt caaatttact caacaattaa tttcttgcag
    10561 aaactctttt ctacatcaaa caccttcaca aaataaattc ccgcagaaat gtttttaaaa
    10621 tttatttctt ttttgctttc tccatccacc acatcaatat aaattttttc ccccatgaca
    10681 ttataaattt caattttacc ttttcggaaa ttctcaggaa agaagattat aaatactcct
    10741 gatgtcggat ttggaaaaaa agaaattgta ttttcattcg gttcaggaat atttatacct
    10801 gttggtaata ttacattgac attaaccata gttccgttgc tgttgctttt gtacaaatat
    10861 gttgatgaat cgcagtaaat gcttgtgcat cctgcaaaat ccgtaatgga aagacaaatg
    10921 ttatagtatc ctgctgtgtt gtatgtgtga gaaggtgttg aaccagaaga attggctgaa
    10981 ccatcacccc agttccaact ataagtgata ggttgttgac ctgttgcctg atttactgca
    11041 atccaattat gagggattat atcagcatat agcgtaaaat aagaagagca actgcaaggg
    11101 tttgtaacaa ttcctccatt gcttgataaa gaagtcacag ttaatgctgc tcctccattt
    11161 gaacttaaag ttgctggatt tgaaatttgc ggaaaaatat ttgttgatat caaagttgta
    11221 gatagttcat tgcatccgac cgttccattt gcatttgttt tgatgaggat taaatcaatg
    11281 cctgaaacag gtaaccactc accagtcaaa gcatagcctc catcagttgt tttacaaata
    11341 cctctttgaa cttcgttact atttataaaa gcatttgccc atatgaaatt acccacgctg
    11401 tcaatcttga aaagaagccc tttgtcaacc attgaaaaag gaaaagaaga ttgaccaccg
    11461 gctaaagcat aaccattatc atttgttcgt actattgatg aagcaacatc tccatattgc
    11521 tcgccataag ttttgctcca aagaatactt cctgtaatat ctgttttaat tacaactgca
    11581 tctgcttcac cggcacctaa aaaactaatt gaataacctc ccataacata accatcatcc
    11641 gatgcctgaa ccacagaata caaccaatca ctgcctcctc ctccaaatgt ttttgaccag
    11701 gtaagagtac ccaatgcatc agttttaata agataataat ctatatctcc agcaccaaaa
    11761 ctgtttgttt cacccgcaac aatataacca ccatcggaag tttgcttaac tgagtttgca
    11821 acatcatcta atgcccctcc aaatgttttt gtccaactaa tattaccatt tccatctgtt
    11881 ttaacaagat agaagtcatt acctcccgct ccccaactat tagttgttcc tgcaattata
    11941 aaaccgccat ccgatgtttg cctaaccgag ttggctcttt cgtttcctac tccaccaata
    12001 attttagacc ataaaatgct tccgttagaa tttaacttta tcagataaat attctcacct
    12061 gcaaaacctc cgctgaaaga agaaatggaa tatccgcata caataaatcc actatcgctt
    12121 gtctgttgaa catcatttgc aaaatcgctc gatgtttcac caatcgcttt tttccaaatt
    12181 ggattgccgg atgaatctgt tttaattgca aaaacatcaa aagcacccac gccaaatgca
    12241 tctgtgactc ctacagaaat gtatccattg tcatttgttt gttgaattga gttaccccaa
    12301 cttgtattag ttcccccaaa acttttttgg aaaggaattt gggcgtttgc taattgagct
    12361 atcagcaaaa ttaaaattaa taacgagagt tttttcatat cagtttgaat ttagcattac
    12421 agtttgttcg gagcgaaagc atatccgata atgacagtct gtgaaaaaac cattttcttt
    12481 tctcctatca acattgttat tatttaagtg ataaaaataa cgaagaaaaa gcaaagtaag
    12541 caacaacatt aatgattttt gttgcatgca gtatatacaa ccacaaaaca gagcacaggt
    12601 aacttttacc agcctggaag acacagtagc tcccaataat gcagttcggc ttattgatgc
    12661 atttgtggag catttagatt tagctcagct aaaatttaat gtttccgttt taaataaaga
    12721 aggccggccg gcttttgagt ctaaggtgtt tcttaaactt tatttttatg gttacctcaa
    12781 tggcatacgt tccagccgca gattagagaa agaaactcaa aggaatgtcg aattgcactt
    12841 gcactcccac tcatcctcgt tgaagtcaag ttcgtgagta agggatgcag atgtgtggta
    12901 ctctataaca ggtgttaatc gcaccctcgt ctctcgcaca caacactact cgtcctcgtc
    12961 tgacgagtgg gggtacacta acatcatgtt taaagaatgc ttactctaac tctatcatat
    13021 tcttggaagt aattacacca ttgatattca gtttgacatt ataaatacct gccggcagac
    13081 caagtttctt cgcactgaag gtatattgat gcattccttt ttcaagtttt tcatttatct
    13141 gtgttgaaac cacctcgcca ataacattaa taacctcaat acttacatca gcattactat
    13201 ttaagaagat gacaaaattg gtttcattct tatatggatt cggggtaata acaaaaatat
    13261 ttgaatcgtc atcaatcttt gcgttgcgaa tttctgtata aacatagctg ccatttttat
    13321 ctacctgttt cagtcggtaa aaataaattg tgtttttgac aacatcttta tcttcgaatt
    13381 tatagtccga tactacatta ctgtttcctt gcgaagtaat gttggcaata gttgtaaact
    13441 caattccgtt agttgaacgc tgtaattcaa aataatcact gtttatttca cttgctgtac
    13501 tccacgctac cataatagaa ttaaccagag gtgaggcatt gaatgcaatt aattctacag
    13561 gtaatggatt gttagcttgt gttattccaa acagttctgt gcctgttcca aagccattca
    13621 tcgctactct atttacggca gtaaccggac tgtttataca aatcccatta aacaaattcc
    13681 atccagcacc gtggtttctc ataatagtcc atcccataaa tggattgctg tagttgagat
    13741 tataggcagt ggcatcatag gttcctgaca aaggactatt gcttgccgta aaactccagt
    13801 aaccattgtc aagcgcaggt tgtgaataaa ccacactaca ttcagtagcc cctattccgg
    13861 cagggataac agaataagta ttaaagaaag aaagaagatt atcaatagtt gtagggcttc
    13921 ccggaacatt aaagttaata ttcattcgct gatatcctgc agttgcatga ccaacaggaa
    13981 aatcgtatga tccttgcgta ttcaggtatc tgcgtaagtt accctgaata aaactattca
    14041 tatttccggg agtaacagct ccgctattcc ggttattaac cgtcacttca aataggttgg
    14101 tgattatttt tccgtttgtc agcgaaagca ttccattatt gccaagcgtt gcattggaaa
    14161 gtaatgttac gcctgtacct gaatgattca tggaaagatt attgagaact ccattgttta
    14221 agtaattctg agcagctgtt ccatacagct caagtgtgcc accaatacct ggtgtgtagg
    14281 taccataatt cagaaaatct cctccaacac gatgtgtttt tgcattggca ttaaaattac
    14341 ttgtacttga agtgataata aaattacctt tcatgtcggc attctgcccc aaagttacaa
    14401 tgccacctgt tttacttaca acaacattag caaatgcaga tgctccaacc atgtttccat
    14461 ttaaactctg atttaccgaa gcgttattaa aatatacatt tgatccgttt gctgcattga
    14521 gcgtaccgtt attattatag tcctgacata cttgtaatga gaacccagca ttgatagaaa
    14581 gcgaggccga aggatttatc gtaatggatt tacaagaagc acctgcagcg ttgattattg
    14641 gcatgtttgc cgatgatggt ggaataatag catctactgt acaatttgga atctggcatc
    14701 caccccagtt aggagcttta aaccaatcgt tgtcaacacc accagaccac actacaaatc
    14761 caggattggg agtgaaatta accggtgagc ttaaaaagtt cattgtgaac cccgatgtgc
    14821 tattggagaa attactcaca acaagctggt aaacatcacc tgcagcaaca ttaatctgtg
    14881 catcataggc tgcattataa tcaggtgtat atggagctgg tgcctgattg gtaacagtgc
    14941 tatttaaccc tgttactcca agatagctgt aattgcaacg tatgggtgct gctccactcg
    15001 ctaaggttgc acatgtatta acagtattat tggtaggtcc gtctttcatc ttccaaattg
    15061 caaaatcata atctgtttca ttacctggta aaccggcaac atagtcatta gggataatgt
    15121 tgaatgctaa tacacctgca ttctgaatat ttatttcata ccaggcagag ccacgctcag
    15181 cacttaaaag acaattacta ccgctgcccg gaaagtcaca tgatgaaccg aaagcctgat
    15241 aacccggatc accgatggat ataatctggt tacaaacagg atttggaaca ctacattcct
    15301 gcccaaatac aggaggaata ttggcgggtg gaccactaac gataataatg ccgtaactac
    15361 ccgtctcatt gttgtaacca tcaactacaa cataataagt tgtacctgct gtcaaaccac
    15421 tataatttat ctgcgacata taattaatgg tagtaccgca tgaaggagca tcatcattgc
    15481 aagccaataa agtcattgat gcaccacagg ttccctgata taaagctatt tgtgaattac
    15541 gtaatgatcc gggcacggtt cttatagtta cctgacctga aggcggtgct acaaaagaat
    15601 gccaaactgt gtttcgtgag cctgctgacc aacatgatgg attagaaggc tcacctgcca
    15661 agcctgaaca gttattactt ccgttcagat aaacaccaat aggaagcgga gttgcattac
    15721 atggtaaatc atttgctgct cctgaaccaa aagattgaat actgatatca taagtaacac
    15781 ttgttggagg tgcccatgta tctacaataa taaaatatgt gcctgctgcc ggcaatacaa
    15841 cactgccgtt taatgttccg gaaagtgctc cgctattatt acctatacag tttgtacctg
    15901 catttcccgg gcagccacta tatacctgat atcctattaa attatcactt gcctgactta
    15961 aagttataga gatacactcg gggccgcttg ctacataacg ataaactttg tcttctcccg
    16021 actcatataa tgtaccgcat gatcctatgg atgcattagt atagtcatta cccatacaag
    16081 ctgttgtttc atttgcagcc gaaaaaggaa gtccacttat atttacagca ttgctgcaaa
    16141 tagccccggg aagcatacct acaggagcac tgatgctgat attatatgaa ggtatacaat
    16201 aaggagatgg gaactggtca actacaagat aataggtatg acctgctata acattaacac
    16261 acatgctctg acttcctgca gaactttgtg caaaagcaac acaggtaccg gcagtaccgc
    16321 tgcaactggt aaccaaagga catccgtcaa acagcgttat tcctacccat gaacttgcgc
    16381 ttgtcagtga aatagttgtc tgtcctgaag ttgagggtgt aaaaataaat acttcgtctt
    16441 cggcaccgtt ataaaaagtg cttccgcagg caatcgtatt ggtagtcgta aaatcatcag
    16501 tcttaccaca ggtagttcga ctgttactgg tataaggcaa cgatgctaca tttactgtac
    16561 cattacctgt tgcgcaggct ccggcaggca caggtgcaga aatggtcaga tcattaaagg
    16621 gaacacaact tggtgcaggc catgtatcaa gaatcagata ataggttgtt cccgcctgaa
    16681 gacagacagt gagcgttttg cttcctgtgg aactctgact gttggcaaca catgttccgg
    16741 gttgaccggt taaagggcag ccattgtata agtttaatgc tgtataactt gctgaaggtg
    16801 cattgagtgt tatggtaaca gttccggtgc tggcaggagt aaaaataaaa actttatcct
    16861 ctccatcaag ataaaatgta ctgccacaag gattggtatt gactgacgtt aaatcattgc
    16921 ctgcaccgca ggtagttcct gcaccactgc tataaggcaa tgaagctatg gctacgttgc
    16981 ctgttccaag atttgaagga caacttaaag gggcactgta cttgtatgac attgttgcac
    17041 ctgtagcgcc tgtagtacaa ctgtattttg taatcaacac cctgtatgtt cctgtggtta
    17101 cacaagtcca tgcgagatag gactgcaatc cgcaattatc atcactaaaa ccacctgctc
    17161 caacataagt acctgcatta ttcaaaatag ttatctgtgt atcccatgta gcatttccac
    17221 catcagcagc acagaatgaa aaataatata caaatcctgc cgtagccgta aatgtataat
    17281 attttcctgc tgccacggca gtggttccgg tggtactcca cgtcactccc ggtgttaaat
    17341 tgctggcaag agtaccacct gtgcattgtg cactgcattc attagctaac agggcataac
    17401 cgacaatcag aaatagtaag agttgtttca tatttgatag gttaataaaa ttattctacg
    17461 tcataatcaa ttattgattc tttatcatat cgctgttgtt tgaatccttg caagcttgtt
    17521 tcactctcga tatagtgaca ccattgcgct ctgtaaatgt gaaaccttca ttcttcagtt
    17581 tattcaataa aattgctctg ctgttatatt cagtagaatt gattattagc ataacacaat
    17641 tcagcgaata acaatattcc tttgaaacaa tgccttctgt tgtacttaag atattgtcta
    17701 tctgcgcatg tcttttctga tttgaaagtt catttattcg gagaattaat tcctgattac
    17761 cgtcatctcc cgcaaatagt aatccattaa ataataaaag gaaggttata agtagtttct
    17821 ttatcatact tttttagttt agttttcgtc cattgtgttt tataggaaaa tcaaggacta
    17881 aaagtatgat aattttaaat taaatcaata tttttctaca tttatttccc atcaatcttt
    17941 ttccaagtgt cctcgtctga agtcaagaac gtgcgtatgg tacgaggacg agtgggggct
    18001 ctatatcagg tgttactcgc atcctcgtct ctcgcacaca acgctacccg tcagacgagg
    18061 acgagggggg gggcatttca tactatcgca gcaacgtaat ccttcctaac ttctcaatat
    18121 agttgttttt taaatcacga tatgaaattt tataaacgta aacaccgaga gggcaatcct
    18181 tactttcaaa cttcccatcc catgaaaaat tattgctatc ttcataaacc acattacccc
    18241 aacgattaaa tattttaaaa ttcgtaatag ttacattgta aaattgtgga gtaaaaaaat
    18301 catttaaccc atcatcgtta ggggtaaaag aattgggcag atacaagcca aacaaagtat
    18361 caggcgggca ttcgtcaata aaaatgctat cgctttcaat agtgccgcag ttgtatttac
    18421 tctgcaacca ataccatcct ttttttgtta ctgatatctg agatgtaatg gctccgttgc
    18481 tccaattata atttggaagt gttacattac ttgaaagcaa aaaaggaaca tttgttccgc
    18541 ggttgcaaat agttgtatct atcccgatgt caattgtaat gcttggaagt gaatctaagg
    18601 taacatgaac actgtctgtt tgcataccgc aaacatctgt tgcctgcaca atgtacaagc
    18661 ccgaatcgtt aactaaaata ttttgtgaag tatctgatgt attccataaa taagaaataa
    18721 aattgggctg cgcagtgagg ttaacagaac taccaacgca aacaatagta tcattaccta
    18781 aatttaattt gtggatattt ttaaaactga tgtgtactgt atcacttatt tctccacagc
    18841 ctgtaagaat acattttacc caatacattc ctgaatcatt aaccgcaatg atgctacttg
    18901 tgccgccatt actccattga tagctgtcat atccaactgg cgcgtataaa ctatatggca
    18961 atgtgttttt gcaaataatg gtgtcgttgc cgagatttgg agcagttaga tttgttggcg
    19021 ttacgctgac atcgtctatg taatagctta ttgaaatagg agtgcctact ggatagaatg
    19081 attgatagga agtgttagcg tataactcaa agttaccaat agtaatgtga ttttcatttc
    19141 cttttgcttt atataatcca gaaatcaaag tccagtttac tgaatcagtt attatagttg
    19201 atgatttaat ctgaggataa atatttatta aaaaattacc tgcaccatta ttccaattaa
    19261 ttaatgtatc agaaattgca gcatcaacat tttttattcc accatatgaa ttattacaaa
    19321 ggtttacaaa ataattaatg caataaatag aatctttttt taaagcgtat ataaaatcac
    19381 cttcaatata atttctttta tcagggtcat agttccaatt gtaagctaaa atacaagcat
    19441 atccaccgcc tgtattagca tattgaaacc cgtatgaatt aataggaacc gtagcacaag
    19501 aatgaaaaaa acttggactt cctggattaa acccaaacca atctatcaca tatccattaa
    19561 ataaacctgc tcctacgggg cattgaacat aattttcaaa cgaagggttt ttaactaaat
    19621 tttgagcata gactacgtta ctctcctctt gtattaataa ttgagcagtt aatgcaaaca
    19681 aatatttgat aaattttttc atggaataaa acacgtatgg caaaacatgc catacgtgtt
    19741 tttattaaaa ttaatttttg gaaatacgac ctgattgatt tactgtactg gatttgatac
    19801 gataggtata aattccgtta ttcagttttg aaatatcaaa cgattttttg aaaacacctt
    19861 tactgatttt tgttaaaggc ataagtagca ttacctgctt gccaagcata tctgttattt
    19921 caatttcata ataatcatct gcgcttgcag aaaattcaac tgttacatta tcacttgccg
    19981 gattgggata aacagaaata ccggaaacat tattttgact ttccgtattt gaatttgggt
    20041 ttgccattct gcatgatgca ttgctcggat taaaataacc aatggctgaa ttgctgctac
    20101 aggcattgcc cacggtaaga gtaatacgat aggttgtgtt taaatttccg gctatatcaa
    20161 aatagccaag ccctaatccc atgtcactgc ctatggcatt catggtttta ggagtaatgt
    20221 ttaccggatt gcaagcagaa acgttataat taggcgtagt gcctaaattg gtaaacccgt
    20281 ttgggcaagc acctgtcagt ttttctaatt ttacattata atagcaaact gttccagtac
    20341 taaagtttgc ataaatgcca ccatctgatg tttgacaaag agcataaggt gcagtgcagg
    20401 tagtgccatt tgcaaaagcc gaaccgccat taccattata caatttaaaa acgctgtttg
    20461 cagccggtgg cggaccaagt atatttatat tttttatctg cgtactcaca ttgccgcagg
    20521 catcttgaat agaaaactta acttgtacag ttccggtaaa tgtacctgtt aatgtaaaga
    20581 gattaagatt tgctgtgggt tgcccggctg tccatagtcc gtgatagtta acacctccgg
    20641 ttgtaagctg gcacccacca tctattgcct gtatatcaaa cttgtattgc gtaggagtgc
    20701 cgttattgta agtggcatta aaaacaagcg gattgcaatt gtaataaaca ggcattgatg
    20761 tggtttcaca gttgcctgat gcactggaac cgtttaatgt aaaatttgca atggtaacgt
    20821 taggcagtcc tgaaaaaaca gcgtagcttt caccgtcaat ctgattaggc aatgtaaaac
    20881 atgtatattg cccaagataa ccgtaggtaa atcgcgaatc aacgaatccg ggattataag
    20941 cactaattgt attagacgat gtattgattc ctgcaaatgt ggttgctgtg agtgtggggc
    21001 cggaaaatgt tggagaaata ccataaatcc tgttattctt accatactcc aaataggtgt
    21061 tggtaaaatt atatccaggt gttaaaattg atgattgcac ctgagtagcc gttagaattt
    21121 gtgttaagcc acctgagccg ctaacataaa gtttggggtt ggtagttgca ttggtaaatt
    21181 caatgcctct tgctgccgtt acattgtaat tttggagtgt gcttggttgg tatgcccctg
    21241 ttgtagaaac aattccaatt acatgcactg ttccatttat atttgaccag gcaagcaggt
    21301 tggtgcccca tgatacttca cactcaaagg catctacagc aggcaatccc aatgtggcat
    21361 aagttgcaac ggttgtacct gcagaaattc cggtattggt aatatcactt cttacaattc
    21421 cgttattgct tacagagaat aaaaaccgct ttgctgttga accgctaccc gtgtaaactt
    21481 tagaaacggc aattgcagta tggtcagctc caccatgccc agaaacccag aaagcctgat
    21541 tcttataacc tacaaagata ggtgggcaat ttacataaac acttccgttg taagttacaa
    21601 caggtgttgc acccgaacaa tctactttta catacagcac aggcgaataa ccaacaggat
    21661 tatccatact gtaaataaca taaaattgat tgcaggtgcc gggaatggga acgattgcca
    21721 cttcagaaca taaaatagaa tattcttcag cacatacctg tgaattgtat ggagctaatg
    21781 ttccaacatt ggaaccggta ggcccgtaaa ttccataatc ccttacaaag aatagtaagt
    21841 tgccgcttgg gtcataagca ccattggcaa ctgagtaagc accgcctcca cccggcaatg
    21901 ccgatgatgt tggaatggat gtggatgtat taatcctgta aggcggagta gtccaataaa
    21961 tcatttgagc gaatgaattt gttactgcca tgagtaacgc aacaattgtt acatttatta
    22021 ttagcttttt cattttttta ttttttaaag aattatatga tgaattaatt tttaggccga
    22081 aaaaagatgc ttagaagact atggtatttt aattgctttt ctcatatctc tttgttttat
    22141 tttccccaca aaaataaatg ccaatacctg ttcataaaaa tatcttttgg ctcatttttt
    22201 ttgagccgtt tggctcattt tattcacaaa gcttgttttc tccgttttga tatattaaac
    22261 ttgccttact ttaaaattac tttatgtttc atcgtggaaa aacagatttt gatgtgctca
    22321 tccttagtaa ggacacattg tttgtagaag tgatggtaga aaaacttaag cctctgtttt
    22381 ctaatatttc tgcaaagcat attcagtccg cagatttaat cggtgtgttt cagttcagaa
    22441 gtttatttat acaccaatca agtgttttag atttttatca aaacaacaat aaatgtaaaa
    22501 atgcatcaaa tgctttttcc atcatctttt ttctttggga aaaaccggag tgttttcaat
    22561 attacccctt aaaatgcaat ttcaaaatta ttgatttaaa aattccggct gccaaaatat
    22621 taaaagaggt aagtcaaata ttactgcccg taaaaagtaa tgaggatgct atatttgaac
    22681 aacttggtgt actcaataaa cagttgtcaa acatacttca aactgctctt ttcactgatg
    22741 atgaattgga tattatttat aagctgaaaa aatttagtga taataaaaca cttgctcaaa
    22801 aatgcaacat ctctattgcc actttaaaaa ggcgcctaaa aattattgct gaaaaatgtg
    22861 ataatgcaaa atccaaatcc gaaatcataa attttcttta tgaaaactat gtaataaaac
    22921 cttaactaaa gtaataatct aaaacctgtt tgtattaaag tcaacaacag caatctttaa
    22981 taaaaataca actacgactt aatcctttac taaaactacc cctctcgtcc tcgtctgaag
    23041 tcaagtacgc gcataaggga cgaggatgtg tgggaattaa aaaaaggtgg aattttagaa
    23101 ttaacgctac aaatgacttg agaactatca atatggttta acctgcattt caatgaaata
    23161 aaaactactt taagcctact ctacatccac caacgtacct acatggttgc cttctaccaa
    23221 acgcaacagg ttgccgggag tattcatatc aaaaactata atcggcaatt tgttttcatg
    23281 acacaaggta aaggctgtca tatccattac cttcaaatct ttttcgtaaa catcatcaaa
    23341 ggtcagatgt tcaaagcgta cagcatcgct gtttttttca gggtcggaag tatagatgcc
    23401 atccactctg gttcctttta aaatcacatc agcctccact tctatggcgc gtaatgaagc
    23461 tgctgtgtcg gtagtaaaat acggattacc tgttccggca ccaaaaatca ctactctgtt
    23521 tttttcaaga tgcctaaccg ctcttcttct gataaaaggc tcgcagatag catccatagt
    23581 aatggctgat tgcaaacgtg tgtttatgcc tttatgttct aatgcgccct gcaaagccat
    23641 actgttaatg acagtagcaa gcatgcccat gtaatcgccc tgcaccctat ccatgccacc
    23701 ttctgctgcc tgcaaccctc tgaaaatatt acctccccct attactatgg caacctgaac
    23761 acctttgtca acaatagttt ttatttcctc cgcatattgg gtaagtctgt tgttgtctat
    23821 cccaaaattt ttctcgccca tcagtgcttc accactaagt ttcaataata ttcttttata
    23881 tttcatatca gatgtttgtt attatctata aacttttttt taatctttcg aaaaataatc
    23941 ggcactttga tacgctcccg taatttgttt taatatctgc atcagcaaat cattggctaa
    24001 tgagcttgca ccaaaccgga acatgtcggc attcaaccac tcctgcccta atgtttctct
    24061 aaccatcacc aaatattgca atgctaactt tgctgttgca atgccaccgg caggcttcat
    24121 gcctattttt attcctgttc tgtaatagta atctcgaata cattcaagca tcaccagggt
    24181 tacaggcaat gttgctgcag gctgcacctt acctgtagat gttttaataa aatctgcacc
    24241 cgctgcaatt gataattcac ttgccttcct tacattatct aatgtggata gctctccggt
    24301 ctccaaaatc actttcagat gtgccttgcc acaggcctct ttcacagcag ctatttcatc
    24361 aaaaacaaaa ttatactcgc ctcttaaaaa ttctcctctc gaaataacca tgtcaatctc
    24421 atcggcacct tctgcaacag cataacgtgt ttcgtcaagc ttaaattttc tgttgctcat
    24481 gccactagga aaagccgttg acacggcagc tactttaacc tttgttccct ctaatgcttg
    24541 tttggcaaca gacactaaat tggcataaac acatacagcg gcagccgaag ggataccttc
    24601 catttcatga ggtctaatgg ctttggcaca aagctgtctt acttttccgg gagtgtcttt
    24661 tgcatcaagt gtggtaagat ccatcattga aatggctaac ttcaatgctt ccatcttaga
    24721 ctcatttttt atactacgct tgtttaatcg tgcaacgcgc tcttcaaccc caacctgatc
    24781 taccggtggc aggtggttga gaaaacgtaa atctgctttt gccatgctga taagataaat
    24841 ttcagagagc taaaataaac attccatatc aattgcagta atatttggtg tcataggata
    24901 aaatacaaat cacaatcagc atttaatttt taaaatctac ctttgcagca ctaaatatta
    24961 tgctatgtct gatgcccttt ttgccgtttc gcctgttgac ggacgctatg ccgctgtttg
    25021 tagtccgctg agagaatatt tttctgaata tgcacttatt cgtgagcgag taaaagtgga
    25081 aatcaaatat cttatagcgc ttgctcaact gcaattgcca cagcttcaga ttgactcagg
    25141 gaaatttgat gcactgcgca gcatctaccg acagtttaca cctgccgatg cagaacagat
    25201 taagcagaca gaaaaaacta ccaatcatga tgtgaaagct gttgaatatt ttgtaaaaga
    25261 aaaattagaa gcattaggct tacataaagc aaaagagttt gttcactttg ggcttacttc
    25321 acaagacatc aacaacactg caacaccaat gcttgtccgt catggcattg ccgaagtaat
    25381 gttgcctgca ttaacagatg tgttaaacaa aattgaaagt caggcacagg actggaacaa
    25441 catcagcctg cttgcgcata cacatgggca acctgcaagc ccaacacgat taggaaaaga
    25501 aatgaaagtt ttcagtgaaa gacttcgtgc acagctagaa atgttgaagt caattcctca
    25561 cagtgcaaaa tttggcggag ctacaggaaa cttcaatgct catgcagtgg catatcctgc
    25621 tgttgactgg atttcttttg ctgatgattt tgttaactcc cttggactta cgcgtacaac
    25681 acatacaaca cagatagaac cttatgacaa ccttgcagca ttattcgata acctgaaacg
    25741 aatcaacaat attttattgg atttatgccg tgatgtatgg acctacatca gcatgaatta
    25801 tttttctcaa actgttaaaa ccggtgagat tggttcttct gcaatgcctc ataaagttaa
    25861 tcctatagac tttgaaaatg ctgaaggtaa tctcggcatt gccaatgcat tgtttgaaca
    25921 tctttcagcc aaacttcctg tatcacgtct tcaacgtgat ttgacggaca gcactgtgtt
    25981 acgcaacaca ggtgttccgt ttgcacacac actcattgca ctgaaatctt tactgaaagg
    26041 tttgaataaa attttgccaa acactcaggt aatttctgcc gaccttaata gtcatgctgt
    26101 tgttatagca gaagcaatac agacaattct tagacgcgaa ggttacccta atccttatga
    26161 agcattaaag gatttaacac gtggaaaaga aaacattact ttacaggata ttcatcagtt
    26221 cataaataca cttcaggtta gcgatgccgt aaaagctgaa ttactgaaaa ttacaccgga
    26281 aaattatacc ggagtaaatt tatcagactg atttttccat
//
LOCUS       DAOQNP010000037        26180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_624_length_26180_cov_7.820708, whole genome shotgun sequence.
ACCESSION   DAOQNP010000037 DAOQNP010000000
VERSION     DAOQNP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26180
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_624_length_26180_cov_7.820708"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKY09_06650"
     CDS             <1..666
                     /locus_tag="PKY09_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12573.1"
                     /translation="VSSCKKKDDDENKLNLNLSFAVDGSKLEFNSIKYVNAAANNYSV
                     SKLHFYISAFEFENADGNKFTFDSIFYVDAATQTPSFGLEGLPVGNYKSLRFLIGLDS
                     LHNISHALPNTIDNENMSWPDMMGGGYHFMKLEGNFMDGGNPTGYAMHLGKNVHVVQV
                     NIQKDFKISGGAAALNLEMNINEWFSNPHNYDFNVDGNYSMSDSVAMSKLALNGKDVF
                     NIQ"
     gene            668..1690
                     /locus_tag="PKY09_06655"
     CDS             668..1690
                     /locus_tag="PKY09_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNB12574.1"
                     /translation="MKLKVIFTVLVVGLLTVLACKKDKDDDPINNLPPYNPTPYTINT
                     PSNLPAMNVPSFNPTTVEGVYLGRKLYYDKLLHPTGTSSCSSCHIQSQGFAVTGTNII
                     PHVNLGYQNKFLWNGEIQGTLEDAMMFEVETFFGTDVSRLQKDSLYPILFYKAFGSTT
                     IDTKKCAYALAQFLRTVVSGNSKLDRFIRHEALFTPDEIQGFNIFFSEKGDCFHCHSL
                     SLTSDGDLHNIGLDSVFTGVNAGYYNVSHNAADYGKFRTPSLRNTALKTTFMHDARFT
                     TLDQVIEHYNTGVKHSPSLDPVMTKPGKELGLQLTPLEKQKLKAFLLTLTDSTFLTNP
                     DFSNPN"
     gene            1719..3095
                     /locus_tag="PKY09_06660"
     CDS             1719..3095
                     /locus_tag="PKY09_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB12575.1"
                     /translation="MNHSQSNNSNTHFLFIAVALSVLIVGVLFGVVGGLQYIIPGWLK
                     DELSFERVRPLHVTMVISWIFAGAMGGIYYYLPQTLKGKLYSPALAKVHLVLFIVTGL
                     SIIACYFAGVFGGREYFEFPPVIAIPMLVTWLLFVANMLLTLKRYYNCNSHIFSKVYI
                     WMWLTGMFFFLLTFLESYLWLFSFFSNNLVRDITVQWKASGAMVGSWNMLVYGTAFYV
                     MEKMSGDEKVASSKLTFFFYFLGLTNLMFNWGHHTYIVPASPWIRDIAYIISMTELLI
                     LGNIIWNWRKTLSSAAKTFHLLPFRFFTASDVWIFINLVLAIAISVPFINRFTHGTHI
                     TVAHAMGTTIGINTFILFASVFYLIGLQGGTTSLKYINAGFWILNISLLIFWISLIAA
                     GVTKTVVLSNGGTFALAIQKMLPYFYSFFIAGIGILAGILLIAIPALVSCIQKVRQSR
                     LVSVQSIA"
     gene            3218..5266
                     /locus_tag="PKY09_06665"
     CDS             3218..5266
                     /locus_tag="PKY09_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12576.1"
                     /translation="MYIFPSQSLLADEFIVVKANIAQSCLTPAAAQEILNLTPSTDKN
                     CIELQLGRTEEMRNILADAEPFPVSGYCDVTKELQLLKIENSVLLPSQALQLAALTHT
                     VGLIIKFFNQVGRYPLLTQILNDCIFEPAIAGEIERVLDSFGVVRSSASTELMHIRRA
                     LQRQRAESDRAYQQVMARYKKNGWLTDSEESSRNGRRVLSIVAEQKRTINGVIHDISA
                     TGKTAFVEPDEVLHINNHIQQLEQDERLEIIRIMRQLTVFLRNYYSTLQQYAFILTQM
                     DMHHAKALFALNYRAIKPEITSKPEINLVNASHPILLIQQKDVQKSVVPFSLHLNHDR
                     RILIISGPNAGGKTVCMKAVGLLLIMAQSGIHITAKAGTSVGVFNKLLIDIGDSQSIE
                     YELSTYSSRLKNMKVFFEQVDSKSLFLIDELGSGTDPQLGGALAEAMLEALNLNRSFG
                     IVTTHYMNLKVLADKVQGFINGSMLFDKRNLKPLFQLQVGKPGSSYTFIVAERSGLPQ
                     QIINSARSKVPKTHMLLEKLLSKVEKDKIVIEEKLKTVLQKEKTLNTLIKQNDELIYK
                     NENLKNSLQQHLRSHQENLDRKAEARIKKFALDLKQAKNKKTVIEKFLSEAGLKNKKK
                     EKVVQRTIDPRIINGAVVKLYNGKVSGIVQSIDQENATVLFGNFTTKCKLTDLILDSK
                     "
     gene            5402..5953
                     /locus_tag="PKY09_06670"
     CDS             5402..5953
                     /locus_tag="PKY09_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNB12577.1"
                     /translation="MKLSSVQTKQLLTTLENRFAENMQRHKGLQWGNVETVLKKSSST
                     LSILWKMEETGGQPDVVSFDKKTGQFTFFDCSPESPTGRRSLCYDNKALDDRKENKPK
                     GSALGLAAEMGVEILSEEQYKFLQTLGKFDLKTSSWLLTPHPIRILGGAIFGDCRYQH
                     VFTYHNGAESYYAARGFRSFITV"
     gene            complement(6191..6588)
                     /gene="ssrA"
                     /locus_tag="PKY09_06675"
     tmRNA           complement(6191..6588)
                     /gene="ssrA"
                     /locus_tag="PKY09_06675"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6700..7407
                     /locus_tag="PKY09_06680"
     CDS             6700..7407
                     /locus_tag="PKY09_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNB12578.1"
                     /translation="MGRIFEKRKHKMFARYAKMAKAFTRIGKEIAIAVKQGGPDPHHN
                     AKLRQVMQNAKSLNMPKDRVDAAIKRASSKDMKDYEEITYEGYAPFGVAILVECATDN
                     PTRTVANIRMHFNREEGNLGTSGSVAFMFERRGLFKFSAEGKNIEDLELELIDFGLIE
                     IWEEDGFIYLYCNFEDFGAMTKVLEDHNIEIINSELTRVPLTLTEVTEEQEQKIQVLI
                     DRLEDDEDVQSVFHNMK"
     gene            7431..8561
                     /locus_tag="PKY09_06685"
     CDS             7431..8561
                     /locus_tag="PKY09_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNB12579.1"
                     /translation="MIIGAGPAGSACVMALKDAGLNIVWIDKATFPRDKVCGDAIPSN
                     VQRVLKSIDGSLNEKFLQHFTEKVQVEGCRFIAPDKTSFDLSFVTKGHAAKRLHFDHF
                     LFNMAKECNSHVSFIGDASVESIVNSATDVTVRLADGRTIEGQVIVGCDGANSIVRKN
                     LAQPFINKKENIAAVRAYYSNVADLEHKMLEIHIIKGYMPGYFWIFPLPENQSNVGFG
                     MVNRFITNRRIDLKEALKEIIHKDKAISPRFSQATIDGVISGFGLPCGGRKNPISGQR
                     FLLCGDAASLINPATGEGIGNAMISGRMAALHIINSFQANNFTQSFNVQYDNMVYSKL
                     LGDLRIQHWLQRITADREWLINFALKHVSNNNWIRQKVRKFF"
     gene            complement(8566..9801)
                     /locus_tag="PKY09_06690"
     CDS             complement(8566..9801)
                     /locus_tag="PKY09_06690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB12580.1"
                     /translation="MENVDVLIIGAGPSGAVASAYLNKQNIKVLVLEKQKFPRFVIGE
                     SLLPHCMDHLSEVGFIDNIKKHGFQEKTGAAFYKGKHKTEFYFNDQFSKGWSWTWQVK
                     RADFDNILISTAIEQGVEVKFECEVNSVDASSASEQTTTYTDKSGKSHTVKSKYIIDA
                     SGYGRVLPRLFKLEEPVSTPPRGAVFCHIHDTKRTKAAGTNIFIHSFNENSAWIWAIP
                     FSDQTTSVGVVSNVELVDEFAANNGAKFLNHIRTFGDLAGRFENEQLVFEPRTIHNYA
                     VRSKQLFGPGYVLCGNSTEFLDPVFSSGVTLATGSGLLAAKLTEKVLNGEQVNWAETY
                     EEKLRRGIDVFRTFVLGWYNGDLQTIIYSDDIDSNIKKQICSVLAGYVWDTENPVVKK
                     HKTLIPTLAEVIRIKEKQA"
     gene            complement(9861..11408)
                     /locus_tag="PKY09_06695"
     CDS             complement(9861..11408)
                     /locus_tag="PKY09_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB12581.1"
                     /translation="MKINENINYDFAIIGGGFAGMVCAYILAKEGFKVALLEKNIQLG
                     GSLQIFSRDKKILDTGLHYIGGYDEGQNLNKLFNYFGLKDTLQTVRMNEDGYDYISFN
                     DGRKYAHAMGYENFISSLCKDFPDEEHAINLFCCKIQEYCHRFPLYDLNNNIADGYFS
                     EDLRNENAWEYISSITTNKTLQNVLAGNNMLYAGVKEKTPLFVHALIFNSYICGSYKI
                     VTGGAQLIKQFSKNIRKAGVDIYTHKQVTGATFGTDNKISQLLFDNGDKISAKNYISA
                     IHPNETISLFGAKRFSNAWVKRVSRLEQTISAFLLQITFKPKSFPYLNYNLYHFFNDD
                     VWETINYTEDNWPLGFFVFTPVPLDNSEFAESISIMCYMKSDDVAQWKDSFNTTTHPS
                     DRCESYQAFKRQKEEKVLAKLETLFPEIRNAMLNVYSSTPLTFKDYTASTDGSIYGIL
                     KNSHSPLASMLQPKTKIPNLWLSGQNIVFHGILGTAISALLTCFEFTDKKELLNKINP
                     EKNHIQT"
     gene            complement(11405..15220)
                     /locus_tag="PKY09_06700"
     CDS             complement(11405..15220)
                     /locus_tag="PKY09_06700"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNB12582.1"
                     /translation="MGSIFIGLYRLFVRKTILFSGFVLLVAAVSIAGIKRLNITENIF
                     AILPKGQEFQKFNTLLESKNISDQVVFSLSLAKDSNPDEYAEVAEHIKSTLDSVSNNL
                     LTDIVAIRPDVQQEVYDYYFSHFPLLIDSNYYSLIAKRTENDSITVSIKSDYEKLTNP
                     SGLFIKQFILNDPLGITAEYFKQLGIIGNSSNYIVEDGFIFTPGKKAILITGRLNYDS
                     RNTEKNIQLSNLLTAAEKKWNTENSEYKLSHFGTFEIAAQNAVQIKKDTNLTMILSLS
                     LIMLILILYYRKLSIPVLFILPAVFGGMIALGMMGFLRPEISAISLATGAIVIGIILD
                     YSFHFFTHLQHTKSIEETLKEITVPLLTGSVTTVLALLALNYTNSVVLGDFGLFASMA
                     LAGSAFFTLAGLPVIITLTRFDYTQHKPIKWFKIPTINPSIVKFTIVAISLITVYFYF
                     EASNIRFNSDVEALSFHPAELKNKEAELTGINPSIEKKIYLIASSENYESAVAANYKA
                     GTLIKNLTTHGIIKNTLSTADYFIPDTLLQQRHDRWKHYWQENKVRVNNQISHAASEA
                     GFSENAFAPFQELIENNTYQPFSKDLLKTMGLDLMVDSNSRESVFISSFTVSTAQKDS
                     VKALFKDIPQLTVFDRSEMASLLLESVRSDFNYLLYLTAGMVFFTLLIIYGRIELTLL
                     AFMPMVVSWIWILGMSSMLNIEFNFVNIVITTFIFGLGDDFSIFVTDGLLHRYKFRKD
                     NLKSYQAAILLSALTVIVGTGVLYFAKHPAIHSIAALSVIGITCILLISFLVQPFIFN
                     LFVERRIRNGHNPISFLQLFMSIFCFSYFISGCLLFYPVLLLITIFPAPVKVKRKVVN
                     FLTSKFAASVLYSGIHVKKKFYNLDQLDFKKPSIIIANHSSFLDIMIMLMLNHKVIIM
                     AKDWVYRSPLFGMVVRYVGYIFSEDGTEENITNIKAKMNEGYSIMIFPEGSRSSDGNL
                     NRFHKGAFYLAEKLNADIQPVMIHGAGDVSPKNEMMVKDGTINVKALSRIYANDLTWG
                     NDYRSRQKSITLYFKEQYKKFKDEREDADYLWRKIYYNYIFKGPVLEWYGRIKWNLEK
                     KNYDFYNSCIGNRLLITDLGCGYGFMDLFLHYKNVERKIVAVDYDEDKIAIASNVWNK
                     NNNLQFKSEDITKTDIQSSDVIFLNDVLHYLKTESQELVLQKCAQALLPEGIIFIRDG
                     ISDDIKHENTKFTELLSTKLFSFNKKTNDLNFFNADFIYQFAKKHNLKVEEHKHSNKT
                     SNRLFVLSK"
     gene            15355..16449
                     /gene="hppD"
                     /locus_tag="PKY09_06705"
     CDS             15355..16449
                     /gene="hppD"
                     /locus_tag="PKY09_06705"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNB12583.1"
                     /translation="MAEDFLPLNGTDHVELYVGNAKQSAYYYQMAWGYELIAYSGPET
                     GVRDRASYVLQQGKIRLVLTSAMHPDSEITRHVAQHGDGVKFLAIWVDDAEKSFYETM
                     SRGAKAHTQPTVMSDQFGEVKISAIHTYGDTVHKFVERKNYKGAFLPGYSPAKSSVKT
                     TSVGLKHVDHCVGNVELGRMNEWVKFYEDVMGFKLLITFDDSDISTEYSALMSKVVSN
                     GNGYIKFPINEPADGKRKSQIEEYIEFYHGAGVQHVAIATDDIITTVTELRNRGVEFL
                     HVPEVYYEEVWDRVGKINEDVATIKKLNILVDRDDEGYLLQLFTKPVQDRPTVFFEII
                     QRCGAKSFGKGNFKALFEAIEREQALRGNL"
     gene            complement(16478..17098)
                     /gene="recR"
                     /locus_tag="PKY09_06710"
     CDS             complement(16478..17098)
                     /gene="recR"
                     /locus_tag="PKY09_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNB12584.1"
                     /translation="MEQYPSRILEEAVNELSRLPGIGRKGALRLALHLLKQNPADVQR
                     LGNAVIQLRSSVVYCQQCHNISENSLCAICTNPRRDNSVICVVQDIRDVMAIENTAQF
                     NGMYHVLGGIISPMDGIGPSQLNIDSLVEKTTNGIINEVIMALSATMEGDTTVFYISK
                     RLKDADVKITILARGVAIGGELEFADEVTLGRSLINRVPYENTLVK"
     gene            17210..18673
                     /locus_tag="PKY09_06715"
     CDS             17210..18673
                     /locus_tag="PKY09_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNB12585.1"
                     /translation="MSPALILSVVLVYTVLLFAITFYTSRNADNQSYFVGNRSSRWYV
                     VAYGMIGASLSGVTFMSVPGDVGTGGFSYFQMVLGYLLGYAVIAFVLLPVYYRMNLTS
                     IYVYLKSRLGEKTYKTGAFFFILSRVVGASFRMYIVVYVLQLFIFQAWGVPFWVTVLV
                     FVLLILAYTFKGGVKTIVWTDSLQTTFMLLSLLISVYLIAKELNFSFADVLSSIKSSP
                     YSDIFVWDWKSKVFFPKHFFGGMFIAIAMTGLDQEMMQKNISCRNVGESQKNMMTFSI
                     ILVFVNFIFLCLGALLYIFIQHQGIVIPERTTDDLFPTIAMHYLGTFSAICFIIGLIS
                     AAYPSADGALTALTASVCIDFLGFEEGKYNEIQKTRIRKSVHIIFGFVLLGVIVLFHA
                     INDQAVIRQIFRAANYTYGPLLGLFAFGILTRRNVIDKAVPVIGILSPVICYIIDINS
                     VQWLGGYKFGNELIILNGGITFMGLLLLTKHKTATQV"
     gene            18673..20103
                     /locus_tag="PKY09_06720"
     CDS             18673..20103
                     /locus_tag="PKY09_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNB12586.1"
                     /translation="MENLQQRSSGTSYNMVTKEILEKLRFICGEKFVVNDDESCTIYG
                     SDETEDLLFKPEVIVKPSDANEISAILKLANTHFIPVTPRGAGTGLSGGALPVHGGII
                     LSTERLNKIIDIDDKNLQCTVETGVINQHLRDAVEAKGLFYPPDPASRGSCFIGGNLA
                     ECSGGPKAVKYGVTKDYVLNCEVVLPTGEIIWTGANVLKNSTGYNLTQLMIGSEGTLG
                     IITKVVLKLIPLPTETLLMLIPFRSAEKACEAVSAVFRAGITPSAMEFMERDAIEFVM
                     KFTDVKVPLKEDHIAHLLIEVDGNNKDVLFKDCEKISEVMYQHDCDEVMFAESAQQKA
                     DLWRARRAVGEAVKSHSVYKEEDTVVPRASLPLLLAGVKKIGDKYGFRSVCYGHAGDG
                     NLHVNIIKDGMTDEEWNRKLPLGIQEIFELCVSLNGTISGEHGIGWVQKNYMSIRFSE
                     GEIALQKGIKKLFDPNNILNPSKIFA"
     gene            complement(20107..22422)
                     /locus_tag="PKY09_06725"
     CDS             complement(20107..22422)
                     /locus_tag="PKY09_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB12587.1"
                     /translation="MSNYLDELNPSQRDAVECMKGPSMIIAGAGSGKTRVLTYRIIHM
                     INNQVDPFNILSLTFTNKAAREMKKRIVEKVGHDAQNLWMGTFHSVFARILRIEAEKI
                     GFPANFTIYDSDDSKSLIKKIINEQNLDDKTYKPSLVLNRISSAKNNLFSVTDYLNHS
                     ELTNEDKMAGKPKIGMLYEIYQKRLKQSDAMDFDDLLFNTYLLLKNHPDALSKYQNKF
                     KYVMVDEFQDTNYAQYIIVRKLAAAHENICVVGDDAQSIYAFRGANIQNILNFEKDYP
                     DLKTFKLEQNYRSTKTIVSAANNVIANNRNQLKKEVWTENTGGEKIRLLRALSDNEEG
                     AQVAQAIFEDKMNLHFKNKDIAILYRTNAQSRSLEEALRKLNIPYRIFGGLSFYKRKE
                     IKDLLAYFRLTINPYDEEALDRIYNYPTRGIGKTTYEKLLVLADARTCTLWDVMQDIN
                     DREIDIASGTKTKINEFVTMMQSFSVLAKTQNAYDVASHIAQSSGLLKELFNDKTAEG
                     ISQYEHIQELLAGIKEFSDGAQTPLEAPSSELEQSEIKLLNEYMQDIALLTDAETDDG
                     NDDKVSLMTIHQAKGLEFKEVFVSGLEENLFPSQLAVNSRDELEEERRLFYVAITRAE
                     KRLTLSYAMTRYRWGTLTGCEPSRFLEEINPEFLDALPVIDNKMSPTAIRQGNGVPSF
                     SSNSFTKNNLKPIVKKPVAKQTDPKLFENFIPDDPKRIEIGMSVLHQRFGKGKVVLLE
                     GNLPDMKATVNFEGEGQKQLLLKYARLKIVE"
     gene            complement(22538..22921)
                     /locus_tag="PKY09_06730"
     CDS             complement(22538..22921)
                     /locus_tag="PKY09_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12588.1"
                     /translation="MITKIEMNHVELLEWLKALDFYKNELRFWRKHLSHVVVSNTNKD
                     ILREAEHFQNQVIIQVDQIDLLHHDVKQFENKLDQEFESVSKGGKFSQQIVDVEASLR
                     ERMKTFEHIYVDLKHDYYSFLRKYM"
     gene            23050..23559
                     /locus_tag="PKY09_06735"
     CDS             23050..23559
                     /locus_tag="PKY09_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNB12589.1"
                     /translation="MKSFIKYGILGALLGFAWTLVGYILGNETQEKLKWLSMLIMIVI
                     VVFCFRAVINEEKKANEGFISFGRAFGMAFKTGLVMAVCSAVFTYLYFNFINPEYMDF
                     AIQKAQEQMQTQGLSDEQIDMSMKMQAKFMTPAVMTIFAFVSSVIIYVIVALIMGAIL
                     KKENPNEMN"
     gene            complement(23556..24788)
                     /gene="rocD"
                     /locus_tag="PKY09_06740"
     CDS             complement(23556..24788)
                     /gene="rocD"
                     /locus_tag="PKY09_06740"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNB12590.1"
                     /translation="METSKETAVAANYFIELEERYGAHNYHPLPVVLSRGEGVFVWDT
                     EGKQYFDFLSSYSAVNQGHCHPRIVTSLVEQAGKLALTSRAFYNDKLGEYEKYVTELF
                     GYDKLLPMNSGAEAVETAIKLCRKWAYKNKDIARNQAKIIFCEGNFHGRTISIISAST
                     DPTASTNYGPFTPGYVIIPYNNLAALEDALSDPNVAGFIVEPIQGEAGVVVPDNGYLS
                     KAAALCRENNVLFIADEIQTGLARTGKMLACDYENVKPDILILGKALSGGMLPVSAVL
                     ANDNVMLTIQPGEHGSTYGGNPLACVVATTALQVILDEKLSENATKLGEILRSELNKF
                     KHEMLTLVRGKGLMNAIVIKPSKGKDAWDVCLKLAANGILAKPTHGDIIRFTPPLVMT
                     EEQLYECIAIIKKTLNEF"
     gene            24948..25931
                     /locus_tag="PKY09_06745"
     CDS             24948..25931
                     /locus_tag="PKY09_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNB12591.1"
                     /translation="MKRKTFSIVLLLCIISLLVYGWGFFAHKRINKIAVFTLPSKLMR
                     FYKVNIDFVEEHAVDPDKRRYAVKDEAARHYIDLDRYGESPLDSLPRNWKAAVEKYTK
                     DTLMAHGIVPWHINVMLGRLTEAFRNKDYNKILRYSADLGHYIADAHVPLHCTQNYNG
                     QLTNQVGIHGFWESRVPELFADDYDYFVGKAQVIEKPMDYIWEIIRQSFAAKDSVLLF
                     EKELNARFDADRKYAFEQRGATMMRVYSVDYSKQYEEMLNGMVERRMRQAIIAVGSFW
                     LTAWTNAGQPNLDGIEKIVISDEMKKEIEAEDKMWRTGKVLSPGGHTDDDH"
BASE COUNT         8070 a         4790 c         4876 g         8444 t
ORIGIN      
        1 gtttcatctt gtaagaaaaa agatgacgat gaaaacaaac tcaaccttaa tttatctttt
       61 gcagttgacg gcagtaagct tgaatttaat agcataaagt atgtaaatgc tgcagctaac
      121 aactacagtg tttcgaaatt acatttctat atttctgctt ttgagtttga gaatgctgac
      181 ggaaacaagt ttacatttga tagtatcttt tacgttgatg cagcaactca aacaccttct
      241 tttggtcttg aagggctgcc tgttggaaat tataaatcat tacgtttttt aattggtctt
      301 gacagtctgc ataacatcag tcatgctttg ccaaatacaa ttgataatga aaatatgagt
      361 tggccggata tgatgggagg agggtaccat tttatgaaac tcgaaggtaa ttttatggat
      421 ggtggaaatc caaccggata tgcaatgcat cttgggaaaa atgtacacgt ggtgcaagta
      481 aacattcaaa aggattttaa aatttcagga ggtgcagcag cacttaatct ggaaatgaat
      541 attaatgaat ggttttctaa tccacataac tacgacttta atgttgatgg aaattattca
      601 atgagtgaca gtgttgccat gtcaaaactg gcattaaacg gaaaagatgt ttttaatatt
      661 caataatatg aagctaaaag taatttttac tgtattggtt gttgggttac tcactgtact
      721 tgcctgtaaa aaggataagg atgatgatcc aataaataat cttcctccat ataacccaac
      781 tccatatact attaatacac catcaaattt accggcaatg aatgttccat cattcaaccc
      841 tacaactgtt gaaggtgttt atcttggccg aaaactatat tacgacaaac ttttgcatcc
      901 tacaggtact tcatcctgtt catcatgtca tattcagtcg caaggctttg ccgttacagg
      961 tactaatatt attccacacg ttaacttagg ctatcagaat aagtttttat ggaatggtga
     1021 aattcaggga acattggagg atgccatgat gtttgaggta gaaacttttt ttggcacaga
     1081 tgtttcaaga cttcagaaag atagtttata tcctattctt ttttataaag catttggcag
     1141 tacaactatt gatactaaaa agtgtgctta tgccttggca cagtttctga gaacagttgt
     1201 ttcaggaaac tcaaagcttg atcgttttat ccgtcacgaa gctttattta cacctgatga
     1261 aatacaggga ttcaatattt tcttttctga aaaaggtgac tgctttcatt gccactcatt
     1321 gtcattgact tccgatggtg atttgcacaa tatcggactt gattcagttt ttacaggtgt
     1381 caacgctggt tattataatg tcagtcataa tgcagcagat tatggaaagt ttcgtacacc
     1441 atcactaaga aatactgcat taaaaacaac cttcatgcac gatgcacggt ttactacact
     1501 tgaccaggta attgaacatt acaatacagg tgtgaaacat tcaccatcac tcgatcctgt
     1561 tatgacaaaa cccggcaaag aattggggtt acaacttact cctcttgaaa aacaaaaact
     1621 caaagcgttt cttctaacac taaccgattc aactttctta acaaacccgg atttctctaa
     1681 tccaaattga gttttattct gattcagtac tacatacaat gaaccatagt cagtcaaaca
     1741 attcaaatac ccacttttta tttattgcag ttgcactttc tgtacttatt gtaggtgtgc
     1801 tgtttggcgt tgttggcggt ttgcagtata tcattcccgg ttggcttaaa gatgaattgt
     1861 catttgagag agtccggcca cttcacgtaa caatggttat atcatggatt tttgccggag
     1921 ccatgggcgg tatctattat tatctgccac agactttaaa aggaaaacta tattcacctg
     1981 ctttagcaaa agtacatctt gtgttgttta ttgtgacagg actttccatc attgcctgct
     2041 actttgcagg agtgtttgga ggacgagagt attttgagtt tccaccggta attgctattc
     2101 caatgcttgt aacctggctg ttgtttgtag ccaatatgtt actgacactt aagcgatatt
     2161 acaactgcaa ttcacatatc ttctctaagg tttatatctg gatgtggcta acgggaatgt
     2221 ttttctttct gttaacattt ttggaatcat atctgtggtt gttttctttt tttagcaata
     2281 acttggtgcg tgatataact gttcaatgga aagcatcagg agctatggtt ggctcatgga
     2341 atatgctggt atatggaact gcattttatg tgatggaaaa aatgagtggt gatgaaaagg
     2401 ttgcctcatc aaagctgaca ttcttttttt atttcttagg tcttaccaat cttatgttca
     2461 actgggggca tcatacctac attgtaccag catcaccctg gattcgggat attgcctata
     2521 ttatcagcat gacagagctg ttaattcttg gtaatattat ttggaattgg cgcaaaacac
     2581 tgtcgtcagc cgctaaaact tttcatcttt taccttttcg tttttttacg gcttcagatg
     2641 tttggatatt tatcaacctt gttttagcta ttgctatttc tgttccattt attaaccgtt
     2701 tcactcatgg tacgcacatc actgttgcac atgctatggg taccactatc ggaataaata
     2761 ctttcatact ttttgcttcg gtgttttatt taattggtct gcaaggtgga acaacttctc
     2821 ttaaatacat aaacgcaggc ttctggattc taaatatttc tttactcata ttttggattt
     2881 ctctgattgc agctggcgtt actaaaacag ttgtactaag taatggtgga acttttgctt
     2941 tggccataca aaaaatgctg ccctactttt atagcttttt tattgcagga ataggtattc
     3001 ttgcaggcat tttacttatt gctattccgg ctttggtttc atgtattcaa aaggtcagac
     3061 agagcagact tgtgtctgtt cagtcaattg cctgagcaga ggctttaaaa atttaataat
     3121 ttgtttggtt tcatagccat gtattaattt cgcaattcgg tattgtgcat accataagtt
     3181 tgagtataac tctgaaaatt tattttgaat aaattacatg tatatttttc catctcagtc
     3241 attacttgct gatgagttca ttgtcgtaaa agcaaatatt gctcagagtt gccttacacc
     3301 ggctgccgca caagagattc tgaacctgac tccaagtact gataaaaatt gtattgaatt
     3361 gcaactcggc cgtacggaag agatgcgtaa tatacttgct gatgcagaac ctttcccggt
     3421 atcaggttat tgtgatgtta caaaagaact tcaactgctt aagattgaaa atagtgtttt
     3481 actgccttca caagcattac aacttgctgc actcacacat actgtaggcc taatcatcaa
     3541 atttttcaat caagtagggc gatatccttt actaacacaa atactcaatg actgcatttt
     3601 tgaacctgcc attgcaggtg aaatagagag ggtgcttgac tcttttggtg tggtgcgttc
     3661 ttctgcatca acggaactca tgcacatcag acgcgcttta caaagacaac gtgcagagtc
     3721 agacagagcc tatcagcaag ttatggcacg ttataagaaa aatggttggt taacagatag
     3781 tgaagagtcg tcacgcaacg gaaggcgggt tctttcaatc gttgcagagc aaaaacggac
     3841 catcaatggt gtcatccatg atatttcagc aacaggaaaa actgcatttg ttgagccaga
     3901 tgaagtgttg cacataaaca atcacattca acagttggag caggacgaga gacttgaaat
     3961 cattcgaatc atgaggcaac ttaccgtatt cctacggaat tattattcca cacttcagca
     4021 atatgcattt atcctcactc aaatggatat gcatcatgcc aaagcacttt ttgcattgaa
     4081 ctatcgtgcc atcaaacctg aaatcacgag taagcctgag ataaatcttg taaatgcctc
     4141 gcatccgata ttattaattc agcaaaagga tgttcagaaa agtgttgtac cgttttcact
     4201 tcatctaaat cacgacagga gaatattaat catcagcgga cctaatgccg gaggtaaaac
     4261 agtctgtatg aaagccgttg gacttttgct tatcatggct cagtccggca ttcatatcac
     4321 tgcaaaagca ggaacatcag ttggtgtttt caataaactt ctcattgata ttggcgactc
     4381 tcaatctatc gaatacgaat taagtacata tagttcacgg ttaaaaaaca tgaaagtttt
     4441 ttttgagcag gttgatagta aatcactctt tctgattgat gagcttggta gtggtaccga
     4501 tcctcaactt ggaggcgcat tggctgaagc tatgcttgaa gcattaaacc taaaccgctc
     4561 gtttggaatt gtaactacac actatatgaa tctgaaagta cttgcagata aagtgcaagg
     4621 ctttattaat ggttccatgt tgtttgacaa gcgtaatctg aaaccactct tccaactgca
     4681 agttggaaag cctggcagtt cttatacctt tattgtggcg gaacgtagtg ggttaccaca
     4741 acaaatcatc aacagcgcac ggtcaaaagt gccgaagaca cacatgctac ttgaaaaatt
     4801 actttctaaa gttgaaaagg acaaaatagt gattgaagaa aagctaaaga ctgttcttca
     4861 aaaagaaaaa acactaaaca cgctcataaa gcagaatgat gagctgattt ataaaaacga
     4921 aaatctcaag aacagccttc agcaacattt acgcagtcat caggaaaatc ttgatagaaa
     4981 ggccgaggca agaattaaga aatttgcatt agacctaaaa caggcaaaga ataaaaagac
     5041 agtaattgaa aagtttctct ctgaagcagg gcttaaaaac aagaaaaaag aaaaggtagt
     5101 tcaaagaaca attgatccgc gtattataaa tggtgctgtt gtgaaacttt ataatgggaa
     5161 agtgtcaggc atagttcagt ctattgatca ggaaaatgca actgttctat ttggaaactt
     5221 tacaacaaag tgcaaattaa ccgacctgat acttgatagt aaataattat tttgctgtat
     5281 cagagttttt atcaatctat attgattgta tttgtacgcc agcaaaattc aaaagatatt
     5341 tttttaattt cacaacccat aaacagtttt catcaaacat aaaatcaagt ttttaatacg
     5401 tatgaaatta tcatcagtac aaacaaaaca actcctgact actttagaaa acagatttgc
     5461 ggaaaacatg caacgtcata aaggattgca atggggtaat gtagaaacag tactaaaaaa
     5521 atcctcttct acacttagca tactttggaa aatggaagaa acaggtgggc aacccgatgt
     5581 tgtttcgttc gataaaaaaa caggtcaatt taccttcttt gactgttccc cggagagtcc
     5641 aacaggcaga agaagtcttt gctatgacaa taaagcatta gacgaccgta aagaaaataa
     5701 accaaaagga agtgcacttg gattagctgc tgaaatgggt gttgaaatat tgtcggaaga
     5761 acaatataaa tttctgcaaa cacttggcaa atttgacctt aaaacatctt cgtggttact
     5821 tacaccgcat cctattcgca ttttaggagg cgccatcttt ggagattgtc gctaccaaca
     5881 tgtttttact tatcataatg gtgcagaatc ctattatgca gcaaggggat tcagaagttt
     5941 cattactgtt tgagcctaat aacctctatg cgcttgacac aattgcaatg cagattgtag
     6001 tattcagaat tatgtattaa agtggatttt aattttttta aaagagtaac ggctctattc
     6061 tcgagatttt atatcataag caacagaaat caaatcatgc ttagttatca gctaaagcag
     6121 gagttttttt atttttcatc tcatcactcc tcacttcatc acatttaact cctcatttgt
     6181 tatacaaaaa aagtggagcc ggtgagattt gaactcacgt ccagatatgg aatccataca
     6241 ctttctacat gcttagccgg tatttaattg tagggataaa aaaagactac ctgcaggtcg
     6301 aatttatcct tagctcttgt tttcttactg ttgcaccaga gcacaacaac agccagttca
     6361 tatctatgat gcccaattcg caaacgctat gaacttaagt ctgcgtagga cagcttgtgc
     6421 taccaccttg tggcagcatt aggcaattct tcttggaaga attaagccgc aagcgcgtag
     6481 tttgtttcgc catttaagcg agttgtgaat cgggatttac gggtcgttct cacagcaccc
     6541 ggcatgctta cgtatttatt acgcacactg tcgaaacctt tcggccccat atttatcaaa
     6601 gaacagaatg caaatttaca gctattctat gacacattta tgaatatttt actgttagtt
     6661 ttttcagaag tgtatttttg cagcaaacca tttaacatta tgggacgaat ttttgaaaag
     6721 agaaaacata aaatgtttgc acgctatgct aaaatggcta aggcctttac acgtatagga
     6781 aaagaaattg caattgctgt taaacaaggt ggccccgacc cacatcataa cgccaagctc
     6841 aggcaagtga tgcaaaatgc caagagttta aatatgccca aagacagggt agatgctgcc
     6901 attaaacgtg cttcgagcaa agacatgaaa gattatgagg agataactta cgaagggtat
     6961 gcaccttttg gcgtggctat tttagtagaa tgtgctacgg ataatccaac acgtaccgtt
     7021 gccaatatca gaatgcattt caacagagaa gaaggcaacc tcggtacatc aggctctgtt
     7081 gcatttatgt ttgaaagacg tggtttgttt aagttttctg ctgaaggaaa aaatattgaa
     7141 gaccttgaac tggaactgat agacttcggt ctgattgaga tttgggaaga ggacggattt
     7201 atatatctgt attgcaactt tgaagatttt ggtgcgatga caaaagttct tgaagaccac
     7261 aacattgaaa ttatcaacag tgaacttaca cgtgttccat taacgcttac ggaagtaaca
     7321 gaagagcagg aacaaaaaat tcaagtgctc attgacagac tcgaggatga cgaagacgtg
     7381 caaagtgttt ttcacaacat gaaataaggt aagacatcaa aacagatgta attatcattg
     7441 gcgctgggcc tgcaggttct gcatgtgtaa tggcactaaa agatgccggc cttaacattg
     7501 tttggattga taaagccact tttcctcgtg acaaagtttg tggtgatgcc attccttcca
     7561 atgtgcaacg tgtgcttaag agcattgatg gttcgctcaa cgaaaaattc ttacaacatt
     7621 ttacagaaaa agttcaggta gaaggttgcc gctttattgc acctgataaa acgtcattcg
     7681 acctgtcatt tgtcacaaaa ggtcatgctg ccaagcgttt acatttcgat cattttcttt
     7741 ttaacatggc aaaagaatgc aacagtcatg ttagttttat tggtgatgca tcagttgaat
     7801 ctattgtaaa cagcgcaact gacgtcaccg ttaggttagc tgatggaagg acgattgaag
     7861 gtcaggtaat tgtcggttgc gatggtgcaa actccattgt cagaaagaat cttgctcaac
     7921 catttatcaa taaaaaagaa aatattgcag ctgtccgtgc atactacagt aatgttgctg
     7981 accttgaaca taaaatgctc gagattcata ttatcaaggg ctatatgccc ggatatttct
     8041 ggatttttcc ccttccggaa aatcaatcta atgttggctt tggcatggtt aatcgtttta
     8101 tcacaaatcg tagaatagat ttaaaggaag ctttaaaaga gattattcat aaagataaag
     8161 ccattagccc ccgctttagt caggcaacta ttgatggtgt tatttcagga ttcgggctac
     8221 cttgcggtgg acgcaagaat cccatttcag gacaaagatt tttactttgt ggtgatgcag
     8281 cttcgcttat caatcccgca acaggagagg gtataggtaa tgcaatgata agcgggcgaa
     8341 tggctgcctt acatatcatc aatagttttc aggcgaacaa ctttacgcaa tctttcaatg
     8401 tacaatacga taatatggta tatagtaaac tcttaggtga tttgcgtatt cagcattggt
     8461 tgcaacgaat tacagcagac agagagtggc tgataaattt tgcattgaag catgtaagca
     8521 acaacaactg gataaggcaa aaagtcagga aattcttctg aaaaattaag cttgcttttc
     8581 ttttattctg ataacttctg caagagtagg tatcagtgtt ttatgctttt taactacagg
     8641 attttctgta tcccaaacat atccggccag aactgaacaa atttgttttt ttatgtttga
     8701 gtcaatgtca tcagaataaa tgatagtttg cagatcacca ttataccatc ctaacacaaa
     8761 ggttctgaaa acatctattc ctctacgtaa tttttcttca tacgtttctg cccaattaac
     8821 ttgctcaccg ttcaatacct tttctgtcag cttggctgcc agcaatcctg atcctgttgc
     8881 aagtgtcacg cctgatgaaa atacaggatc taaaaattca gtgctgttac cgcaaagaac
     8941 atatccgggg ccaaataact gttttgatcg tacagcataa ttatggattg tgcgaggctc
     9001 aaaaaccaac tgttcatttt caaaccttcc tgctaaatca ccaaatgttc tgatatgatt
     9061 caaaaacttc gcaccgttat ttgcagcaaa ctcatcaacc aactcaacat tacttaccac
     9121 cccaacagaa gtagtttgat cactgaaggg aatagcccaa atccaggcac tgttttcatt
     9181 aaatgaatgg ataaaaatat tggtacctgc tgctttggtt cgtttggtgt catgaatatg
     9241 gcaaaagaca gcaccgcgtg gcggagtgct cacaggttct tccaacttaa ataaacgagg
     9301 tagcacacga ccatatccgc ttgcatcaat gatgtatttc gatttaacag tatgtgactt
     9361 tccggattta tccgtatagg ttgttgtttg ttcacttgca gaagaagcat caacggaatt
     9421 tacttcacat tcaaacttta cttctacacc ttgttcaata gctgttgaaa taagtatgtt
     9481 gtcaaaatca gcacgtttta cctgccatgt ccacgaccag cctttgctaa actgatcgtt
     9541 aaagtaaaac tctgtcttat gtttcccttt ataaaatgca gcacctgtct tttcctgaaa
     9601 tccatgtttc ttgatattgt ctataaatcc aacctctgag agatggtcca tgcagtgcgg
     9661 taataaactt tcgccaatta caaaacgagg aaatttttgc ttttccagca caagcacttt
     9721 tatgttttgc ttgttcagat aggcagaggc aacagcaccg gaagggcccg caccaataat
     9781 cagtacatct acattctcca tttataggtt gttaaaaatt ttattgcgca gcaaatttaa
     9841 tagtaaacct tatttgcctt ttaagtttgt atatgatttt tttcaggatt aattttattt
     9901 aacagttctt ttttgtctgt aaattcaaag catgtcagaa gcgcacttat tgctgtaccc
     9961 aaaatgccat gaaagactat gttttgccct gatagccaaa ggttcgggat ttttgttttt
    10021 ggttgtaaca tacttgctaa tggcgagtga ctgtttttta atattccata aatggaacca
    10081 tcagtactgg cagtgtaatc cttaaaagtc agtggcgttg aactgtaaac attcaacatg
    10141 gcatttctaa tttcaggaaa taatgtttct aacttagcca aaaccttttc ttccttttgt
    10201 cttttaaaag cctgataact ttcacagcgg tcagatgggt gtgttgttgt gttgaaacta
    10261 tctttccatt gggcaacatc atcagatttc atatagcaca ttatagaaat tgattcagca
    10321 aattctgaat tgtctaacgg aacaggagtg aaaacaaaga agcctaatgg ccagttgtct
    10381 tctgtataat taattgtttc ccaaacgtca tcattaaaaa aatgatagag attgtaattg
    10441 agatagggaa atgattttgg tttaaaagta atttgtagaa ggaatgcaga tatagtttgc
    10501 tccaatcggc ttaccctctt gacccatgca tttgaaaagc gcttcgcacc aaaaagtgaa
    10561 atagtttcgt tagggtgaat tgcagaaata taattttttg cagatatttt atccccatta
    10621 tcaaatagca gttgagaaat tttattgtca gtaccgaatg ttgctcctgt aacttgttta
    10681 tgcgtgtaga tgtcaacacc ggctttacgt atgttttttg aaaattgttt gattaattgt
    10741 gcaccacctg taacaatctt atagcttcca cagatataac tattgaagat taatgcatga
    10801 acaaacaaag gtgtcttttc cttgacacct gcatacagca tattgtttcc tgccaataca
    10861 ttttgcaatg ttttattggt tgttattgat gaaatatact cccatgcatt ttcatttctc
    10921 aggtcttcgc taaagtaacc atcagcaata ttattattca ggtcgtataa tgggaaccta
    10981 tggcagtact cctgtatttt gcagcaaaaa aggtttatgg catgttcctc gtccggaaaa
    11041 tctttacata gtgaactgat gaaattttca taacccatag catgtgcata ttttctgcca
    11101 tcattaaaac tgatgtagtc atagccatcc tcattcattc taactgtttg taaagtgtct
    11161 tttaggccaa aataattaaa taacttatta agattttgtc cttcatcata accgccaata
    11221 taatggagac cggtatcaag aatttttttg tcacgactga aaatctgaag gcttccacct
    11281 aactgaatgt ttttttcaag caaagcaacc ttgaagcctt ctttagcaag aatatatgca
    11341 caaaccatgc cggcaaaccc tcctccgata attgcaaagt cgtaatttat attctcatta
    11401 attttcattt gctaagtaca aaaagccggt ttgatgtttt gttggaatgt ttatgttctt
    11461 caactttcag attgtgtttt tttgcaaatt gataaataaa gtcagcgttg aaaaaattga
    11521 gatcatttgt ctttttatta aatgaaaaaa gttttgttga caataactct gtaaatttag
    11581 tattttcatg tttaatgtca tctgatatgc catcacgaat aaaaattatt ccttcgggta
    11641 gcagggcttg tgcacatttc tgtaatacca attcctggct ttctgttttt aaataatgca
    11701 aaacatcgtt aagaaaaatt acatcagacg attgtatatc tgtttttgta atatcttccg
    11761 atttaaattg taagttgttg ttcttattcc aaacatttga tgcaatggca atcttgtctt
    11821 catcataatc taccgcgaca atttttcttt caacattttt ataatgaaga aacaagtcca
    11881 taaatccata accacaacca aggtctgtaa tcaataatct gtttccaata caactgttgt
    11941 agaagtcata atttttcttt tccagattcc atttaatgcg gccataccac tctaagaccg
    12001 gacctttgaa aatatagttg taataaattt tacgccagag atagtctgca tcttcccgct
    12061 cgtctttaaa ctttttatat tgttctttaa aatataatgt aattgatttt tgtctgcttc
    12121 tgtaatcgtt accccaagtc aaatcatttg cgtatattct tgataatgct tttacattta
    12181 tggtgccatc tttaaccatc atctcattct ttggcgagac atcacctgca ccatgaatca
    12241 ttaccggttg aatatcggca ttaagttttt ctgcaagata aaatgcacct ttatgaaaac
    12301 gattaagatt gccatcagat gaacgagagc cttcagggaa aatcataatg gaataacctt
    12361 cattcatttt agcttttatg ttggttatgt tctcctctgt tccatcttca gaaaaaatat
    12421 agccaacata gcgcaccacc attccaaaca acggtgaacg ataaacccaa tctttagcca
    12481 tgataattac tttatgattc agcataagca ttatcataat gtcaaggaaa gaggagtggt
    12541 tggcaataat aattgaaggc tttttaaaat caagttgatc aagattgtaa aatttctttt
    12601 ttacatgaat acctgaatac agaactgatg cagcaaactt tgaagtaaga aaatttacaa
    12661 cctttctttt gactttaacg ggtgccggaa aaatagtgat gagcaacaga acaggataaa
    12721 ataaaaggca gccggaaata aaataactga aacaaaaaat ggacatgaat agttgcagaa
    12781 atgaaatagg attgtgtcca tttctgattc tgcgttctac aaagagatta aaaataaatg
    12841 gttgcacaag aaatgaaatc agtaaaatgc aggtaatgcc tataacactt aatgctgcga
    12901 tagagtgaat agcaggatgc tttgcaaaat acaatacacc ggttcctaca attactgtca
    12961 aggctgataa taaaattgct gcctgataag attttaaatt atcttttctg aatttgtaac
    13021 gatgcagtaa accatccgta acaaaaatgc tgaaatcgtc acccaatccg aaaataaatg
    13081 tagtaataac aatatttaca aagttaaatt caatattaag cattgatgac attcctaaaa
    13141 tccaaatcca acttactacc attggcataa acgcaagtag agtaagttca atgcggccat
    13201 aaataatcag taaggtaaaa aacaccatac ctgcagtaag atacagaagg tagttgaagt
    13261 cgctacgaac agactcaagc agcaatgatg ccatttcgct tcggtcaaaa actgtcagtt
    13321 gtgggatatc tttaaataat gctttaacag agtctttctg tgctgttgac actgtaaatg
    13381 atgaaataaa gactgattct ctggaatttg aatcaaccat taagtctaag cccattgttt
    13441 tcaataaatc ttttgaaaat ggttgatagg tgttattttc aatcaattct tgaaatgggg
    13501 caaacgcatt ttcactgaaa ccggcttcac ttgcagcatg actgatttga ttgtttacac
    13561 gcaccttatt ttcttgccaa taatgtttcc atctatcatg acgttgctgt aatagtgtat
    13621 caggaataaa ataatctgct gtggacaaag tgtttttgat gatgccgtgt gtagtcagat
    13681 tctttattaa tgttcctgct ttgtagtttg cagcaactgc agattcataa ttttcagagg
    13741 aggcaatcag atatatcttt ttttcaattg atggattaat acccgttaac tcagcttctt
    13801 tatttttcaa ttcagcagga tggaaactta aagcttccac atcgctgttg aaacgaatgt
    13861 ttgatgcttc aaaataaaaa taaacggtaa tcagtgagat ggcgacaatg gtaaatttta
    13921 ctattgatgg attgatggtt ggaatcttaa accacttaat gggtttatgt tgtgtgtaat
    13981 caaagcgggt taaagttatt ataacaggta gtcctgcaag agtaaaaaat gctgaacctg
    14041 ctaatgccat tgatgcaaaa agtccaaaat ctcccaagac aacagagttc gtatagttta
    14101 acgcaagaag tgctaataca gttgtaacac ttcctgtaag cagcggcaca gtaatttctt
    14161 ttaaagtctc ctcaatggac ttggtatgct gaagatgtgt aaaaaagtga aatgaataat
    14221 caagtatgat tccaataaca atagctccag ttgccaatga aatggctgaa atttcaggac
    14281 gaagaaatcc catcattcca agtgctatca tgccaccaaa tactgcaggc agaataaata
    14341 gaactggaat gctaagcttt cggtaatata aaatcaggat gagcatgatt agtgataagg
    14401 aaagaatcat ggtcaggttg gtgtctttct ttatctgcac agcattttgt gctgcaattt
    14461 caaaagtacc gaagtgtgat aatttgtatt ctgagttttc agtattccat tttttttctg
    14521 cagcagtaag taggttgctt agttggatgt ttttttcagt attgcgactg tcataattca
    14581 ggcggccggt aatgaggata gcttttttgc ccggagtaaa aatgaaacca tcttcaacaa
    14641 tataattact gctgttgcct atgatgccta attgtttaaa atattcagcc gtaataccta
    14701 aagggtcatt cagaatgaat tgtttgataa ataaaccgga tggattggta agcttttcgt
    14761 aatcgctttt tattgaaaca gtgattgaat cattctctgt cctttttgca atcaatgaat
    14821 aataattgct gtcaatcagc agtggaaaat gcgaaaaata gtaatcataa acttcctgct
    14881 gtacatcggg tcttattgca acaatgtctg tcaataaatt attggaaacc gaatctaaag
    14941 tgcttttaat atgctctgcc acctcagcat actcatcagg gtttgagtct tttgctaaag
    15001 ataaagaaaa tactacctga tcagaaatat tttttgattc aagaagagtg ttgaatttct
    15061 gaaactcctg accttttgga agaatggcaa atatgttttc agtaatgttg agccttttaa
    15121 ttcctgctat gctaacagca gcaactaaca gaacaaaacc tgaaaacagg atagtctttc
    15181 ttacaaacaa acgatataat ccaataaaaa tacttcccaa gaagaactga ttttaaaaca
    15241 aaactttaag agtgcgaatt tatatgaatt attgtgtcaa actttaataa aacctttata
    15301 tactttcatt ttaaaaatga ttacttttgt ctctcattct aatattaaat caaaatggca
    15361 gaagattttc ttcctttaaa cggaaccgac cacgtagaac tatatgtagg caatgccaag
    15421 cagtctgcat attattatca gatggcctgg ggctatgaat taatagccta ttcagggcct
    15481 gaaaccggtg ttcgtgacag agcatcgtat gtattacagc aaggtaaaat acgcttggtg
    15541 ttgacaagtg ccatgcatcc tgatagtgag attacaagac atgtagcaca acatggcgat
    15601 ggtgttaagt ttttagctat ttgggttgat gatgccgaaa aatcattcta cgaaactatg
    15661 agccggggtg caaaagcaca cacacaacct actgttatga gtgaccagtt tggcgaagta
    15721 aaaatttctg ccattcatac ctatggtgat actgtacata aatttgtaga aagaaaaaat
    15781 tacaaagggg catttttgcc gggatattct ccggctaaga gcagtgtaaa aacaacttct
    15841 gttgggctaa aacatgttga tcattgcgtg ggcaatgttg aactggggcg catgaatgag
    15901 tgggttaaat tttatgaaga tgttatggga tttaagttgc taattacctt tgatgatagc
    15961 gatatttcaa cagaatattc tgctttaatg agtaaggtgg ttagtaatgg taacggttat
    16021 attaaattcc cgataaatga gcctgcagat ggcaaacgca aatcacagat tgaagaatac
    16081 attgaatttt atcatggagc aggagtgcag catgttgcta ttgctactga tgatattatt
    16141 acaacggtaa cagaattacg aaatagggga gtggagttct tacatgtacc tgaggtttat
    16201 tatgaagaag tttgggatcg tgttgggaaa attaacgaag atgttgctac cattaagaaa
    16261 ctgaatattt tagttgatcg tgacgatgag ggttatctgc ttcagttatt caccaagcct
    16321 gttcaggaca gaccaacagt attttttgaa atcattcagc gttgcggtgc taaatctttc
    16381 ggaaaaggaa acttcaaagc tctttttgaa gcaattgagc gcgaacaggc attgagagga
    16441 aatttgtaaa gattaatggc caatgaataa ttggcagtta ttttactaat gtattttcgt
    16501 aaggtacacg gttaattagc gagcggccca atgtcacctc atcggcaaat tccaattcgc
    16561 ccccaatggc aacgcctcgt gcaagtattg taatcttaac atcagcgtct tttaatctct
    16621 tggagatata aaacacagtt gtgtcacctt ccatggtagc acttagcgcc attattactt
    16681 cgttaataat tccgttagta gttttttcaa ccaacgaatc aatattcagt tgcgaagggc
    16741 caataccatc cattggagaa ataatgccgc caagaacatg atacatacca ttaaattgtg
    16801 ctgtgttttc aattgccata acatctctaa tatcctgcac aacacaaatc acactgttat
    16861 cacgtcttgg atttgtacag atggcacata aagaattttc agaaatatta tgacattgct
    16921 ggcagtaaac taccgatgaa cgtaattgta ttacagcatt acccaaacgc tgtacatcgg
    16981 cagggttctg cttaagcaaa tgcaatgcaa gacgcaaagc acctttacga ccaatgccgg
    17041 gcagacgcga aagttcgtta actgcctctt ctaatatacg tgacggatac tgttccacct
    17101 gaggtaaatt taaatgatgt tgtcaaatat acgttgttta aaaataccat cttgcttgcg
    17161 ttgtcaaagg ttttcaaaca gttattcttg tattctaaat ttaaaacatt tgagtccagc
    17221 attaatttta tcagtggttt tggtttatac cgtcctgctg tttgcaatta ctttttacac
    17281 ttctcgtaat gccgacaatc agagttattt tgttggtaac agaagttcac gatggtatgt
    17341 ggttgcatat ggaatgattg gtgcatcgtt atcaggtgta acatttatgt cagtgcccgg
    17401 tgatgttggc acaggaggtt tttcatattt ccaaatggtg cttggttatt tgctaggata
    17461 tgctgtcatt gcttttgtat tgttaccagt ctattaccgc atgaatctga cttcaattta
    17521 cgtttatctg aagtcaaggc ttggagaaaa aacttacaag acgggtgcct tcttctttat
    17581 tctttcgcgt gttgttggtg cttcgttcag aatgtacatt gtagtatatg tgttgcagtt
    17641 atttattttt caggcctggg gtgtcccttt ctgggtgact gtattggtgt ttgttttact
    17701 gatacttgca tatacattta aaggtggtgt aaaaacaatt gtgtggacag attcattgca
    17761 aacaactttt atgttgcttt cgcttttgat ttctgtttat ctgattgcca aagagcttaa
    17821 tttcagtttt gctgatgtgc tttcttcaat caagtcaagt ccatattcag atatttttgt
    17881 ttgggactgg aagtcgaaag tgttttttcc gaaacatttt tttggaggta tgtttattgc
    17941 cattgctatg acaggacttg accaggaaat gatgcagaaa aatattagtt gcaggaatgt
    18001 tggtgagtca caaaaaaata tgatgacatt tagtatcatt ttagtgtttg tgaattttat
    18061 tttcctctgt ctgggtgcat tgttgtatat tttcattcag catcagggca tagttattcc
    18121 tgaacgtacc actgatgatt tgttcccgac aatagccatg cattatctcg gcactttttc
    18181 agctatatgc tttatcatcg gtctgatttc cgctgcttat ccaagtgccg atggtgcact
    18241 gacagcactt acagcctctg tctgtattga ttttctcggt tttgaggaag gtaaatacaa
    18301 tgaaatacaa aagacacgaa tcagaaaaag tgttcacatt atttttggtt ttgtattgct
    18361 gggtgttatc gttttgtttc atgccataaa tgatcaggct gtaatacgtc agattttcag
    18421 agcagcaaat tatacctatg gcccgttgct tggtcttttt gcttttggaa ttcttaccag
    18481 gagaaatgta attgataaag cagtacctgt cattggaata ttatctcctg ttatctgcta
    18541 tattattgat ataaactctg tgcagtggct tggtggttat aaattcggga atgagttaat
    18601 aattcttaat ggcggcatca cctttatggg cttacttttg ttaaccaaac ataaaaccgc
    18661 aacacaagtg taatggaaaa tttgcagcag cgtagtagtg gcacatcata taatatggtc
    18721 actaaagaaa ttcttgaaaa actaaggttt atttgtggtg aaaagtttgt tgtcaatgat
    18781 gatgaatcat gtacaatcta tggtagtgac gaaacagaag atttgttatt taaacctgaa
    18841 gtaatcgtaa aaccttctga tgccaatgag atttcagcaa tattgaaact tgccaatacc
    18901 cattttattc ctgtcacgcc tcgaggtgca ggtaccggtt taagcggtgg agctttgcct
    18961 gtgcatggtg gaattatttt atctacagag aggttaaata aaattattga cattgatgat
    19021 aaaaatcttc aatgcactgt cgaaacaggg gtaattaatc agcatttgcg tgatgccgtt
    19081 gaagccaaag gattgtttta tcctcctgac ccggcaagca gaggaagttg ttttattggt
    19141 ggtaatcttg cagagtgtag tggtgggcct aaggctgtga aatatggtgt tactaaagat
    19201 tatgtgttga attgcgaagt agtgctccca acaggcgaga taatatggac aggtgccaat
    19261 gtgcttaaaa attcaacagg atataatcta actcaattga tgataggtag tgaaggtaca
    19321 ttaggtatca ttacaaaagt ggtgttgaag ctaattccat taccaacaga aactttgttg
    19381 atgctgattc cttttcgttc tgccgaaaaa gcatgcgaag ctgtttcagc agtgttcaga
    19441 gcaggaatta caccttctgc tatggaattt atggaacgtg atgccattga atttgtgatg
    19501 aaatttactg atgtaaaagt tccactgaag gaagatcata ttgcacattt acttattgaa
    19561 gttgatggca acaacaaaga tgttttattt aaggactgcg aaaaaatttc agaagtgatg
    19621 tatcagcacg attgtgatga agtgatgttt gctgaaagtg cacagcaaaa agcagatctc
    19681 tggcgtgcaa gacgcgcagt aggcgaagct gtaaagtcac attctgtgta taaagaagaa
    19741 gatacagttg tgcctcgtgc aagtttgcct ttattgcttg caggagtaaa aaaaatcggg
    19801 gacaaatatg gtttccgaag tgtttgttat ggacatgcag gtgatggaaa tctgcatgtc
    19861 aacattatta aagatggcat gactgatgaa gagtggaatc gaaaattgcc tttaggtatt
    19921 caggaaatat ttgaactttg tgtgtcgctt aatggaacca tttcaggaga acatggaata
    19981 ggatgggtgc aaaaaaatta tatgtctata cgtttttcag aaggtgaaat tgcacttcaa
    20041 aaaggaataa aaaagctttt tgaccccaat aatattttaa atccttcaaa aatttttgcc
    20101 tgaatcttat tcaacaatct tcaatctcgc atacttcaac aaaagttgtt tttgtccttc
    20161 accttcaaag ttcaccgtag ctttcatgtc aggcaggttg ccttccaata gtactacttt
    20221 tcctttgcca aaccgctgat gtaatacact cattccaatt tcaattcttt tcgggtcgtc
    20281 aggaataaag ttctcaaaca attttgggtc ggtttgtttg gcaacaggtt ttttgactat
    20341 tggcttaaga ttgttttttg taaaactgtt ggaactgaag ctggggacac cattaccttg
    20401 tctaatagct gttggagaca tcttattgtc aataaccggc agtgcatcca aaaattccgg
    20461 attaatttct tcaagaaagc gactgggttc gcagcctgtt aatgttcccc aacgataacg
    20521 tgtcatggca tagcttagtg taagacgctt ttctgctctg gtaatggcaa catagaataa
    20581 acgcctttct tcttcaagtt cgtcacgcga gttaacagcc aactgcgatg gaaataaatt
    20641 ctcttccagc ccggaaacaa aaacttcctt aaactctagc cccttagcct gatgaatggt
    20701 catcaatgaa actttatcat catttccatc atcagtttct gcatcagtta gcaaggcaat
    20761 gtcctgcata tattcattca gcaattttat ttctgattgc tccagttctg atgatggtgc
    20821 ttcaagaggt gtttgtgcac catcgctaaa ctctttaatc cctgcaagca actcctgtat
    20881 atgctcgtat tgactgatac cttctgctgt tttatcatta aataactctt tcaacaatcc
    20941 acttgactgg gcaatgtgac ttgccacatc ataagcattt tgagtctttg ctaaaacaga
    21001 aaaactttgc atcatagtaa caaactcatt gattttagtt tttgttcctg atgcaatatc
    21061 aatttcacga tcgttaatat cctgcatcac atcccacagc gtacaagtgc gtgcgtcagc
    21121 aagtactaat aacttttcat aagtggtttt tccgatgcct cgggtagggt agttatatat
    21181 tctgtcaagc gcttcttcgt cataaggatt tatagtaagt cgaaaatagg ccaacagatc
    21241 ttttatttct tttcgcttat aaaaagataa tcctccgaaa attctgtatg gaatatttaa
    21301 tttacgtaat gcttcttcta aacttctcga ctgtgcattg gtgcgataaa gtatagctat
    21361 atccttattt ttaaaatgca gattcatttt atcttcgaag atagcctgtg ccacttgtgc
    21421 accttcctca ttgtcgctca atgctctaag tagtctgatt ttttcgccac ctgtattttc
    21481 tgtccatact tcttttttaa gttgatttct gttgttggca attacattat tggcagcact
    21541 aactatggtc ttggtgcttc tataattttg ctccaactta aaagtcttta aatccggata
    21601 gtctttttcg aagttgagaa tattttgaat gttagcgcct ctgaatgcat agatactttg
    21661 tgcatcatca cccacaacac aaatattttc atgtgctgct gccaatttcc tgactataat
    21721 gtactgtgca tagttagtat cctgaaactc atcaaccata acatatttaa acttattctg
    21781 atacttcgac aatgcatcag gatgattttt cagaagcaaa taggtgttga ataacaaatc
    21841 atcaaaatcc atggcatcag actgcttcag tcgcttttga taaatttcgt aaagcatacc
    21901 tatctttggt ttgcccgcca ttttatcttc attggtcagt tcggaatgat tcaggtagtc
    21961 ggttacagaa aatagattat tctttgctga cgagattctg ttcagcacta atgagggttt
    22021 gtaggttttg tcatcaagat tctgttcatt gataattttt ttaatcagac tttttgagtc
    22081 gtcagaatca taaatggtaa aatttgcagg gaacccaatt ttctcggcct caattcttaa
    22141 aatacgagca aacacagagt gaaaagtacc catccataaa ttttgagcat catgaccaac
    22201 tttttcaacg atgcgttttt tcatttcacg ggctgcttta ttcgtaaagg ttaaagacag
    22261 aatattaaaa gggtcaacct gattgtttat catgtgtatg attctgtagg taagcactct
    22321 ggtttttcct gaacccgcac cggcaataat catacttggg cctttcatgc actcaactgc
    22381 atcacgctgc gatggattta attcatctaa ataattactc ataatggaat ttactacttt
    22441 tcaaagataa ggtatgcttt ggttattaaa atgaaaacag ggtatgtttt ttcataccct
    22501 gctattgaaa actttttaaa gaaacttaaa ctacagatta catatatttt ctcagaaaag
    22561 aatagtaatc atgtttcagg tcaacataaa tatgttcaaa tgttttcatc cgctcgcgaa
    22621 gcgatgcttc tacatcaaca atttgttgtg aaaattttcc acccttactc actgattcaa
    22681 actcttggtc taatttgttt tcgaattgtt ttacatcgtg atgtaacaag tctatctggt
    22741 caacctgaat gataacctga ttctgaaaat gttcagcttc gcgaagtata tctttgtttg
    22801 tattgcttac aactacatgc gatagatgtt tgcgccaaaa tctcaattca ttcttgtaaa
    22861 aatccagtgc cttcaaccat tcgagtaatt caacatggtt catttcaatt tttgttatca
    22921 tacattatgt tcttttgtag ctgctaaaat agcacatttc cgatttactt tggttacagt
    22981 catgacatta aatttctttg tatgcattaa aaaactaatt ttggcttgta aatattaaat
    23041 caaaacaaaa tgaaatcatt tatcaaatat ggaatattag gtgccttgct tggttttgct
    23101 tggacattag taggttatat tttaggtaac gaaacgcagg aaaaactaaa atggcttagt
    23161 atgttgataa tgattgtcat tgttgtattt tgtttcaggg cagttatcaa cgaagagaag
    23221 aaagcaaacg aaggttttat ttcatttgga agggcttttg gcatggcatt taaaaccggc
    23281 ttggttatgg ctgtatgcag tgctgttttc acttacttgt atttcaattt cattaatccg
    23341 gaatacatgg attttgccat tcaaaaagca caggagcaaa tgcagacaca aggcttatct
    23401 gatgagcaga ttgacatgtc aatgaaaatg caggctaaat ttatgacacc ggcagtgatg
    23461 acaatatttg cctttgtcag ctcagtaatt atttatgtaa ttgttgcatt gataatgggt
    23521 gctatactga aaaaagagaa ccccaatgaa atgaactaaa attcatttaa tgttttcttg
    23581 attatcgcaa tacattcata aagctgttct tctgtcatca ccaatggtgg tgtaaatctg
    23641 ataatatcac catgagtggg cttggctaaa atgccatttg ctgccagttt caaacaaaca
    23701 tcccaagcat cttttccttt acttggttta atcacaatag cattcatcaa tcctttacct
    23761 ctaaccagag taagcatttc atgcttaaat ttatttaact cactacgaag tatttcacca
    23821 agtttagttg cattctccga taacttttca tcaagtatta cctgcaatgc tgttgttgca
    23881 actacacagg ccaatgggtt tcctccatag gtagaaccat gttctccggg ctgaatcgtc
    23941 agcataacat tatcatttgc taaaacagca ctcaccggaa gcatgccacc actcaatgct
    24001 tttcccagaa tcaaaatatc cggttttacg ttttcataat cgcaggcaag catttttccg
    24061 gtacgtgcca atcctgtttg aatttcatca gcaataaaca aaacattatt ctctctgcac
    24121 aacgctgctg ctttcgataa atagccatta tcaggaacaa caaccccggc ttcaccctgt
    24181 ataggctcaa caataaagcc tgccacgtta gggtcggaga gggcatcttc caatgctgcc
    24241 agattattat atgggattat tacatacccc ggagtaaacg gaccatagtt tgttgatgca
    24301 gtagggtctg tcgaagcaga gataatcgaa atagttcgcc catgaaagtt cccttcacaa
    24361 aaaataattt ttgcctgatt gcgtgctatg tctttattct tatatgccca ttttctgcat
    24421 agtttaatag cagtttctac agcttctgca cccgaattca tcggcagcag tttatcatag
    24481 ccaaacaatt cggttacata tttttcatat tcaccaagtt tgtcattata aaaagctctt
    24541 gacgtaagtg ccaactttcc tgcctgctca actaacgaag tcacaatgcg tggatgacaa
    24601 tgaccttgat tcactgcact gtatgacgat aaaaaatcaa aatattgttt gccttctgtg
    24661 tcccagacaa aaacaccttc gccacgactt aacaccaccg gcaacggatg atagttatgt
    24721 gcgccataac gctcttccag ttcaataaaa taatttgctg ctacagcagt ttccttactc
    24781 gtttccataa taaaaaattg tctgttgcaa atttaatcat ttgacatcac tttaatggta
    24841 agcatctgaa ttatggcaca atctaagcac gcaaataatc aatagcactt ctgcttaaaa
    24901 tgtcaaatca attaaaaaca actattttta acgcttcaac aatttacatg aaaagaaaaa
    24961 ctttttcaat tgtcttgctt ttatgcatca tcagcttgtt ggtttatggc tggggatttt
    25021 ttgcccataa acgaattaac aaaatagctg tctttacctt gccttctaaa ttaatgcgct
    25081 tctacaaagt caacattgat tttgttgaag aacatgccgt tgaccccgac aaaagacgtt
    25141 atgctgttaa agacgaagcc gcacgacact acattgacct tgaccgctat ggcgaatctc
    25201 cactcgacag cctgcccaga aactggaagg cagcggtcga aaaatatacc aaagacaccc
    25261 ttatggcaca tggcattgtg ccttggcata ttaatgtaat gttgggcaga ctcaccgagg
    25321 cattccgaaa caaagactat aacaaaattc ttcgctactc tgccgaccta ggacactaca
    25381 ttgccgatgc acatgtgccg ctacattgca cacaaaacta taacggacaa cttaccaatc
    25441 aggtaggcat tcacggcttc tgggaatcgc gagtcccaga actctttgcc gatgattacg
    25501 actacttcgt tggcaaagca caggtcatcg aaaaacctat ggactatatt tgggaaatca
    25561 tccgccaaag ttttgctgca aaagattccg tattgctatt cgaaaaagaa cttaacgccc
    25621 ggtttgatgc cgaccgcaaa tatgccttcg agcaacgtgg tgccaccatg atgcgtgtct
    25681 attctgttga ttactcaaaa caatatgagg aaatgctcaa cggtatggtc gaacgcagaa
    25741 tgcgtcaggc aatcattgcc gtaggcagct tctggctcac agcatggacc aatgcaggcc
    25801 agcctaatct cgatggcatt gaaaaaattg ttatcagcga tgagatgaaa aaagaaatag
    25861 aagccgaaga caaaatgtgg cgcaccggca aagtgctttc accgggcggc catacagacg
    25921 atgatcattg atttttttta tagtaacaac caagcactga cactatcaca atgaaccttg
    25981 tactacaata tcaacttata agttatacaa cttttaaatt ttgtaaaagt taaaaacgca
    26041 aagttaaaaa tttatccttt ttaactttgc gtttttaatt gttgctttca tttattgttg
    26101 ttttttattt gaaccaaact tttctttcac tctatctact acataataca ccattggcac
    26161 taagaaaatg gttaatacca
//
LOCUS       DAOQNP010000038        26050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_627_length_26050_cov_9.139219, whole genome shotgun sequence.
ACCESSION   DAOQNP010000038 DAOQNP010000000
VERSION     DAOQNP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 26050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26050
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_26050_cov_9.139219"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKY09_06750"
     CDS             complement(<1..193)
                     /locus_tag="PKY09_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12592.1"
                     /translation="MKGIANLMKLAFVSALMLTVGDINAQTTITYTANGTFTVPAGVT
                     TVKVEAWGGGGAGGGAAPAL"
     gene            complement(486..1385)
                     /locus_tag="PKY09_06755"
     CDS             complement(486..1385)
                     /locus_tag="PKY09_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNB12593.1"
                     /translation="MNSCKRILIAGGSGFIGKQLTQLFIQNGMEVHILSRTKKAVSGA
                     QTFYWSTESNLIDDACLEGVDAIINLAGDGIVDRPWSDKRKTEIYNSRINATKLLFDL
                     VYKHKNQVTAYIGASAIGVYPEGINLTENTLPADTFLAKLCHQWELEHLMFEQRGIRT
                     CIVRIGLVMSPQGGMLKEILKSFRFFIAPVFGNGNQWQSWISINDLCGLFFYLLSNNQ
                     LNGIYNGVAPCPLMAKDMIQQIRNSQKMPGVLVHIPSGVLKLMMGERAEMLLANQQVM
                     SDKVQAIGYNYIHRDFSSIFKQK"
     gene            1621..2895
                     /locus_tag="PKY09_06760"
     CDS             1621..2895
                     /locus_tag="PKY09_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNB12594.1"
                     /translation="MDRFFGIFGFLFILGIAYLMSNNKKAINLRLVLSGLGLQLLLAF
                     FILKTSIGQMLFHKIADFITKILDYALSGGEFVFGVLIKKETLAEVFGPASSFIFMFQ
                     IMPTIIFVAVLVSIAYHIGLMQRIVSVFAKGMKAIMNVSGAEALSNVSSAFVGQVEAQ
                     ILIKPYISKMTMSELLASMTGSMACIAGGVMAVYISMGVPAEYLLAASIMAAPAALVI
                     SKIVYPETEESETKGTVKLEIKKTHSNLMDAISHGASDGLKVSLNVIAMLIGVIALIA
                     LIDATLGFCGRHLADWGLSLSAINIDLAKLSLKEILGAVFSIFAYLMGVPSQDVHIAG
                     QLLGTKLAVNEFVAYVDFTAAMKTMSPKAITILSIALCGFANFSSVAIQVGGIGELAP
                     SRRADLAKLGLKALVCGTLASYLSATLAGILM"
     gene            2970..3641
                     /locus_tag="PKY09_06765"
     CDS             2970..3641
                     /locus_tag="PKY09_06765"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB12595.1"
                     /translation="MFRLTFILLLLSITCKAQQDAQITGVISDNDNKEPLPGVMISYG
                     TNELTQAVFTDDKGSFKIRPLAPGSYNIRFSILGYETLSTIGVRVSDGETVVLNKTLN
                     FSNTLPVVRKEEYRIPLMPRNPNTPLVISYEDIKYAVDKNINALAASTAKVFQADAGE
                     PISMSGSRYGTVKYIVDGVITENEPKLPGCAIEQISIYNSSIPACYGDATGGIIVITS
                     KSYRF"
     gene            complement(3646..4734)
                     /locus_tag="PKY09_06770"
     CDS             complement(3646..4734)
                     /locus_tag="PKY09_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNB12596.1"
                     /translation="MSTEIYNLKYRKAIIIWLLTGCLLIAAMVVIGGITRLTGSGLSI
                     TEWKLIHGTIPPLNEKEWMEEFDSYKAIPQYQLLNYNFSLEDFKQIYFWEYFHRLLGR
                     VIGMVFFFPFLFFWIKKQFNNERLRKMLLLFFLGGLQGFLGWFMVKSGLTERTSVSHY
                     RLAIHLISAFITFGATFYVALDYIFPQRREYDLHLKKLYKLSWITMIVLCVQIIYGAF
                     VAGLKAGRIFNSFPKMDDHWISDAVGMSLQKLGAAALFDAMPVVQFIHRYVAYAVVIL
                     FLVLYFTAKNLNTARLNKLLYPEVLSTLWLLPALVVLQFILGVFTLLYGVPVWLGVVH
                     QATAFLLFGMMVLLLHRFKTTCKPEITT"
     gene            complement(4731..6005)
                     /locus_tag="PKY09_06775"
     CDS             complement(4731..6005)
                     /locus_tag="PKY09_06775"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNB12597.1"
                     /translation="MKVFKFGGASVKDATSVKNAVEILKGFKGEKLLVVISAMGKTTN
                     ALEKVVNDYFFSKGNAHTSLKEVRDYHQQIMEELFSEKTNPVFDKVNNFFVEADWMLD
                     EKPQHTYSFVYDQIVSLGELISTCIVSAYLEQQGCSNIWLDVRDVLMTDNTYREAKVD
                     WQKSEMLVQKQVVPVFNRVDVIITQGFIGGTSENYTTTLGREGSDYTAAIFAYLLDAE
                     AMYIWKDVPGVLNADPKFYKDAQKLDQISYHDAIELAYYGATVIHPKTIKPLENKNIP
                     LYVKSFLKPEANGTAIRTNVQTKPLIPSYIFKNNQVLISIAAKDFSFIAEDNLSRIFA
                     LFAGHGVKINLMQNSAISFSVCVDNDSFKIPALIEALQKDYKIRYNEDLMLYTIRHYY
                     QNTIDAITSGKEILLEQRSRNTAQFVLRESVK"
     gene            6151..6636
                     /locus_tag="PKY09_06780"
     CDS             6151..6636
                     /locus_tag="PKY09_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNB12598.1"
                     /translation="MDILIIIISALLLLAGIAGCILPVLPGLPIAFGGLLLADWHFNF
                     YTGRFLWIMFAMVIIISILDYYLPIWTAKRFGATRYGVIGSFIGMLLGIFLTPVGMIM
                     GTIIGAIVGDFLAGKNTRDALRSGLATVGGTFISTGIKLVGAGIMTWHFVKSTISYYA
                     D"
     gene            6675..7175
                     /locus_tag="PKY09_06785"
     CDS             6675..7175
                     /locus_tag="PKY09_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNB12599.1"
                     /translation="MKAIIKGQEINLNFDSKKKVLTQNNQQVDIDIKKISESSLSILL
                     QNKSYKAELLEANYTEKQFKIRVNGNVYQVALKDKYDELLQQLGMDKMFAQKVNDLKA
                     PMPGLVLDILVSEGQTIQKGDSLLVLEAMKMENNLKAINDAVVKKINVSKGDKVEKNT
                     VLIELS"
     gene            7187..7897
                     /locus_tag="PKY09_06790"
     CDS             7187..7897
                     /locus_tag="PKY09_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNB12600.1"
                     /translation="MKNRRDFIKTSALAAGTLALYGIGSKVAFASENNEDNLFKLPDL
                     EYGFEALEPYIDTLTMEIHYTKHHQGYVNNLNKAVVAEKLQGVSLNELLGKVSKHSAA
                     IRNNAGGHYNHSFFWRLMRPGGSKTPTNALAEAITKDFGNIDNLQKVFSDAAKNRFGS
                     GWAWLIKSTDGKLSVTSTPNQDNTLMDIAEVKGTPVLGIDVWEHAYYLHYQNKRADYV
                     NAWWNLINWDVVLENYNA"
     gene            complement(7902..9035)
                     /locus_tag="PKY09_06795"
     CDS             complement(7902..9035)
                     /locus_tag="PKY09_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB12601.1"
                     /translation="MNLLRLKLAIIALFLAWLLPQAEVSAKNQPVDIVFTLDLSGSTN
                     GLIDDVRDNIWGMNNELTRLYPGTDIRFAVVGYSRPSFGGKNQFVKVISPFTSNIDFI
                     ATELYKLKPNIEKGDQYVGAAIRASLDLLSWSHEKDAVKQIFLTGNGSVFLGAFDVVE
                     SCNLAKEKGIAVNSLYCYSSLRSKEISGWYKISEITGGKSIDVKVHKRLPDYATVTDF
                     NRLQMLAAELNKTYIYYGKAGRDKFKAMVSNEKNALNARHSTFEDLLYHKISDRFQGK
                     QSDWDLVDFLKSRNGNLKNVDAHFLPDSLKNINPEQLLTKLMILKERRSYLLSQIRQL
                     LPFERQDKLTSYFNTKQSDSDMIFDRQVMIVLKDAIKSDLAAN"
     gene            9133..9507
                     /locus_tag="PKY09_06800"
     CDS             9133..9507
                     /locus_tag="PKY09_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12602.1"
                     /translation="MNETNIQKIKHKWGELLRQLEETFGEKPDLQGMIFLIGVQELGK
                     GYQNFTKEEKQDLMHIATCKLLSYEGFYKLIGVDADGWPQYEKAKTMPQMPLKEQDLL
                     LKINIIRYFEEDGYELEKSEVI"
     gene            complement(9500..9865)
                     /locus_tag="PKY09_06805"
     CDS             complement(9500..9865)
                     /locus_tag="PKY09_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12603.1"
                     /translation="MKSSLRKTHLVLTSLIVMTVLFTFSCKKEGPCEAIITITDSAGV
                     AVKGATVILRQDAVVNPTTGVQANVYQEGVTDGYGKASFEFKLEAVLNIEASKGPKLG
                     KDYIRLEQSKTVYRTVIIK"
     gene            9953..10645
                     /locus_tag="PKY09_06810"
     CDS             9953..10645
                     /locus_tag="PKY09_06810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNB12604.1"
                     /translation="MKKIICIIATILPLIALSQTDKSQAKIQSEKSQNKETMVEITTN
                     LGTMRFKLYSETPLHRDNFIKLANEGFFDSTLFHRVIKNFMVQGGDPQSKNAAAGAML
                     GNGGPGYTIPAEFNTALYHKKGALAAARLGDQVNPQKASSGSQFYIVQGKPAADNELT
                     MMENAKHMKYTPEQRKEYTTVGGTPFLDMDYTVFGELVSGMDVLDKIAAEATNPQNRP
                     LQDIRMTVKVIK"
     gene            10759..11565
                     /locus_tag="PKY09_06815"
     CDS             10759..11565
                     /locus_tag="PKY09_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNB12605.1"
                     /translation="MAKKQSLGKRIIIVILLFFGIAVLAFFYRNQIIRYGYKAWRLEK
                     RLFNKENHAATKISFPKGYAVHGIDVSRWQTDINWSQLKAADLNGDTINFSFAFIKAT
                     EGILLEDPSFIDNWAGAQKNNIIRGAYHYFLPNADVSLQAKNFISSVKLKSGDLPPVI
                     DVEEKGNLTKKALVKKLKQFITIIEKQYKVKPIIYSNISFIEDYLADDFPDYNFWIAH
                     YYEDELKIEKSVKWLFWQHSDRVSIPGSSSSYDANVFNGSKNDLKKLLIK"
     gene            11673..12245
                     /locus_tag="PKY09_06820"
     CDS             11673..12245
                     /locus_tag="PKY09_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNB12606.1"
                     /translation="MNQIKQKIILASRSPRRQQLLQMMGIAFEVKTKETDEDYPEHLK
                     NEQIVLHLASKKAIAFENEIQENELLISADTIVVHQNKVLNKPADYNEAIEMLTTLNG
                     SMHEVYTGVCIASIHKKELLYDKSEVYFRNYTVEDMHYYITNFKPYDKAGSYGIQDWF
                     GLTCIEKINGCFYNVMGLPTRKVYQQLINF"
     gene            12249..13139
                     /locus_tag="PKY09_06825"
     CDS             12249..13139
                     /locus_tag="PKY09_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNB12607.1"
                     /translation="MRKAYLQMHISILLWGFTGIFGKAIGMNAIMIVWYRMIISAVAL
                     IPFMIKHKQFILPDRKRLASIIITGFVVCLHWLTFYASIKASNVSIALSCFASVSLFS
                     ALLEPLFYRKKIDKQNIILALFVLVGIYIIFAFQQLYAMGIILALISALLGALFTILN
                     KIFVSNDEPAPVTFIELISGFVFLSVLLPLILPAFNLSFQIPSKIDWVWLLLLSVVCT
                     SVAFTLSLEALKKVSAFTMNLSVNLEPLYSIVLAIILFKENELLNNGFYIGAVIVISS
                     VFIHSLMQYRVRDKSITEQG"
     gene            complement(13119..14405)
                     /locus_tag="PKY09_06830"
     CDS             complement(13119..14405)
                     /locus_tag="PKY09_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB12608.1"
                     /translation="MMQATLEKGAYTASDMAETLIGSEIIKLAGEVNEQIKKGIKIFN
                     LTIGDYDPAIFPIPDSLKNEIIKAYQDGYTNYPVANGQPELRSAVAGYINKLQKLQYS
                     SNEILISGGARPLIYALYKTVVNPGEKVVFPIPSWNNNHYCHLAKAEAVAVETQPNNN
                     FMPLASELKSHLSSAALVALCSPLNPTGTVFSSAQLMEICELILQENKRREGKKPLYL
                     MYDQIYMALTFGKTKHVDPVSLLPEMRPYTIFIDGLSKAFSATGLRVGWAFGPAGIID
                     KMKSILSHVGAWSPKAEQVATAHFLTQENEVDVFLSHYKAEVEKRLSAFYDGFLKMKK
                     DGLPVNAIDPQAAIYLTVQFDILNKVTPDGKRINNSNDITSFLINYATVALVPFSAFG
                     AETTSNWFRLSIGTARMEDIEQMFISLRKALALLGD"
     gene            14567..15706
                     /locus_tag="PKY09_06835"
     CDS             14567..15706
                     /locus_tag="PKY09_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNB12609.1"
                     /translation="MEFAATENQQMIAQTVKDFTEKHIRPNVMTWDEAQEFPLDVFKK
                     MGDIGLMGILVPEQYQGAGLGYFEYVNAIVEVARVCGSVGLSMAAHNSLCTGHILAFG
                     NEEQKKKWLPKLATGQWIGAWGLTEANTGSDAMRMQCVAKQDGDSWIINGAKNWITHG
                     KSGDVAVVLARTGELLDSNGITAFVVERGTPGFKAGKKENKLGMRCSETAEMIFEDCR
                     VPASNILGKVGDGFKQAMKILDGGRISIAALSLGIAKGAFDASVKYAKERQQFGQPIA
                     NFQAIGFKLADMATKIEASELLILQAADLKNRGEKVTRQSAYAKYYASEVAVYVSTEA
                     VQIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVIARELLK"
     gene            complement(15775..16653)
                     /locus_tag="PKY09_06840"
     CDS             complement(15775..16653)
                     /locus_tag="PKY09_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12610.1"
                     /translation="MKRLMLIATGMFLCLTAVEAQPYKQAGGENNLQLLFAPFGSTPL
                     SLNEGITYRRFLKDGKLAARLSISISSSKSTDVIMQADDTLAFPTTTTVFHAEGGGGY
                     IQLTTGMNPQADLVSKTSGFSFRPGIEKHFEGTDRLSPYIGAEISFSSSKTKFTRDTI
                     LPTDYTSPTFYDTTSTTVIVSAPWTTKSISGSSGSKTFGVNLIAGMDYYFSKNLSLGA
                     EFGFGFYSTKYDDLKSDYLDVKNTWTESVPDANGVSTITAAQTTTTKSNPSMKQGKDS
                     GFGPNVTAKIKLGWLF"
     gene            complement(16769..18157)
                     /locus_tag="PKY09_06845"
     CDS             complement(16769..18157)
                     /locus_tag="PKY09_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12611.1"
                     /translation="MKNRLIIFWNFLIVCSLFLSCASAPEKKEAVQQVDSSTVVQTHF
                     TKIKLEPGKILSNIKSESNSNVSFSVYVPSEYNDTTLPPVVYFFDPHADGNLPLEKYK
                     SVADAFKFIMVGCNSVENGMPFEQSQQIANVLFSDCQNRLPINMQQQIIAGFSGGSKV
                     ACNLAMNQHFFSGLIACSGALFENGKFDKNMSVVSVAGINDFNYHEMVALEKSVDVTH
                     HAFIETDSTHQWPDKSDMYDAMLFTLMQMVKQNKLQIQKTVIENYYQTWLKKINSLQN
                     KSSVDKQFKQLQFGVVCFDGLTDVASLKSKLKEIENSESLRKWRMRSQNLQYQEKEMQ
                     KMLRDAFSNQNISWWQKEIADLKIAAKNSNDKMLAAMSNRLLGFIGIACYSFAKQLVN
                     ENAPESDKVLQIYQTVEPNNSEAWFLSAVYHANNKQPEKAMAVFNKAVQSGFNQTSRI
                     NEYPALQQIVAP"
     gene            complement(18147..18677)
                     /locus_tag="PKY09_06850"
     CDS             complement(18147..18677)
                     /locus_tag="PKY09_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNB12612.1"
                     /translation="MALIKPVNGVMPKFGKNFYAAENATIVGQVVMGDDCSVWFNAVV
                     RGDVHSITVGNKVNIQDGAIIHCTYQKAPTVIGNNVSIGHNAIVHGCTLEDNVLIGMG
                     AIVMDHAIIKSNAIIAAGSVVLENTVVESGCIYAGVPAKKVKEVSPEHFKDLNERIAN
                     NYVMYSGWFNNDGDEK"
     gene            complement(18749..19870)
                     /locus_tag="PKY09_06855"
     CDS             complement(18749..19870)
                     /locus_tag="PKY09_06855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB12613.1"
                     /translation="MLFLLIIPALYVFLSFKWMLGFNTIANKKQPVQPHQINNFFSIV
                     IAVRNEQANMERLLHSLTDLNYKKADFEIIIVDDHSTDGTATLATQLLNIYNLNGTVI
                     SLQNSKGKKSAIAEGIKISKAEVIITTDADCVFNPDWLKGINNEFNLSKPDMLILPVV
                     IQGKGVLSKMQQTESLGLAALTFGSLAIGKPLMCNGANLVFKKETYLQINNAEMRSDI
                     ASGDDTFFMLSLFAKNNQSIHALASDKVTVMSDAQSGLSELINQRVRWASKLKYYKQQ
                     CIIITGLFLTMFSILQYAAIPGVIFLPLNQLWLVSAITIAVKWICDFIFIQNAKSKLN
                     QKFYPGAFVLMQLLFPIYTFVVGVLSLSDHYQWKGRSYQ"
     gene            complement(19967..20845)
                     /gene="atpG"
                     /locus_tag="PKY09_06860"
     CDS             complement(19967..20845)
                     /gene="atpG"
                     /locus_tag="PKY09_06860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNB12614.1"
                     /translation="MANLKEVRLRIASVNSTQQITKAMKMVSAAKLRRAQDAIMQMRP
                     YAANLREILENLSQSLSSDGSPFTKVRPANNILIVSISSNRGLAGAFNSSVIKATSKL
                     INETYQKQAKEGKVKVLAIGKKSADFFAKNQNVYFGNENSLYNALNFANTAVIAERIM
                     DEFAKEKWDRVILVYNQFKNAAVQILQTEQLLPIIPPASQEKSKTVSDYIFEPNKESI
                     VSDLLPTAVKTQLFKAVLDSHASEHGARMTAMNKATDNAGELIKDLRLQYNKARQAAI
                     TNEILEIVAGAEALNG"
     gene            complement(20908..22485)
                     /gene="atpA"
                     /locus_tag="PKY09_06865"
     CDS             complement(20908..22485)
                     /gene="atpA"
                     /locus_tag="PKY09_06865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNB12615.1"
                     /translation="MVEIRPDEVSAILRQQLSGFKTSSDLQEVGTVLQVGDGIARIYG
                     LTNVQSGELIEFENGLKGVVLNLEEDNVGAVLLGTATDIKEGDTAKRTGLIASINVGE
                     GMLGRVVDTLGNPIDGKGPIKGETFEMPLERKAPGVIFRQPVNEPLQTGIKAIDAMIP
                     IGRGQRELVIGDRQTGKTAVCIDTIINQKEFYDAGKPVYCIYVAIGQKGSTVANIVKT
                     LEERGAMPYTVIVSASASDPAPMQFYAPFAGAAIGEYFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKINASDSIAQQMNDLPDSLKGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEANLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFAKFGSDLDAATKNVLDKGARNVEILKQGQY
                     SPLRVEQQVAIIYLGTKGLLRNVDVKKVREFETNYLSLLEATQKPILDRLKKGEFDDS
                     ITSVLEKVAADLAPKYS"
     gene            complement(22518..23072)
                     /gene="atpH"
                     /locus_tag="PKY09_06870"
     CDS             complement(22518..23072)
                     /gene="atpH"
                     /locus_tag="PKY09_06870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNB12616.1"
                     /translation="MDTKVAGRYAKSLLGLAIEQKTEQKIYADMELMVSTISSSRELA
                     LLLRNPIINSDKKQNILSSIFKGKVSDVTYAFINIIVAKRREGYLEDITRQFINLYKA
                     HMGIATAVVTTPFKLDDSLRAEIMKIVSAEAKGKVELVEVIDKDLIGGFVLKMNNKQV
                     DTSIETKLREIRRELKINLYEKNY"
     gene            complement(23083..23577)
                     /locus_tag="PKY09_06875"
     CDS             complement(23083..23577)
                     /locus_tag="PKY09_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNB12617.1"
                     /translation="MELLKPAFGLTFWMVLGFLIVLFLLTKFAWKPILNALNERDKSI
                     EDALNAAIKAKDEMANLKSDNEKLLAEARQERDKILKEARVTKDNIINEARDKATAEA
                     DRLLTLAREGIHNEKMAAITDLKNQVALLSVEIAEKIMKQQLADDGKQKALVADLLKD
                     VKMN"
     gene            complement(23662..23916)
                     /gene="atpE"
                     /locus_tag="PKY09_06880"
     CDS             complement(23662..23916)
                     /gene="atpE"
                     /locus_tag="PKY09_06880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNB12618.1"
                     /translation="MLSTILLEVAGNLGIGYGVAALGAGLAALGAGVGIGRIGGSAVE
                     AIARQPEASGDIRANMILTAALVEGAAFFAMVVGLLVVFK"
     gene            complement(23947..25089)
                     /gene="atpB"
                     /locus_tag="PKY09_06885"
     CDS             complement(23947..25089)
                     /gene="atpB"
                     /locus_tag="PKY09_06885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNB12619.1"
                     /translation="MKNLNIRILISAILFWLVPFISMAQESHDSLSVATTESIESAEF
                     DAGRMIMDHISDAHGWHLWGESEIPLPIIVYSPERGLSVFSSGHFHHGHSTYNGYMLK
                     EQKIVAVDEMEETDAHHATINESLTANLYDFSITKNVFALFVSIILMIWIFTSVAKSY
                     KTRPNQAPKGLQSLIEPLIVFVRDDVAKSAIGPKYQKYMPYLLTAFFFIWINNLLGLV
                     PLIPGGANLTGNIAITMSLAVMTLIITVFSANKNYWLHIFAMPGVPMWVLFLLTPIEI
                     LGLFLRPFVLMIRLFANMAAGHIIALSFISLIFIFGEMSAGAGMGVGVISLAFMVFMT
                     FLELLVAFLQAYVFTLLSAIYIGAAVEEHHHDAEHHDAKVEEALII"
     gene            25222..25938
                     /gene="bshB1"
                     /locus_tag="PKY09_06890"
     CDS             25222..25938
                     /gene="bshB1"
                     /locus_tag="PKY09_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNB12620.1"
                     /translation="MKLDILAFGAHPDDVELACSGTLAKHHKMGHKTGVADLTDGELG
                     TRGSPELRRKEALNAAQEMGLDVRHFLGFRDGFFQNDEAHRIKVIEVLRHFQPDIVIT
                     NAPSDRHPDHGRGCNLVSDSCFLSGLRKIETSFKNHKQQPWRPKLVLHYIQDRYFKPD
                     IVVDISKFFEVRMNAILAYKSQFHDPESAEPKTYISSPEFIDSLKSRPAEFGRLIGVD
                     YAEGFLFSRITGINDLKSLI"
BASE COUNT         8267 a         4931 c         4662 g         8190 t
ORIGIN      
        1 caagtgcagg tgcagcacca ccacctgcac caccaccgcc ccaggcttct acctttactg
       61 tggttactcc ggcaggaacg gtaaaggttc cattagctgt ataagtaatt gttgtttgag
      121 cattgatgtc acctactgtt agcatcaatg ccgagacgaa ggctaacttc atcagatttg
      181 cgattccttt catttgtaac tgtttttaat tgttattgat tgtttttcga aatttagttc
      241 atgcaattta aatagcgtgt atgacctgag tggtaatatt tgggcgaata gcaaaatatt
      301 atgggtaaaa tttaaccaat ccaatgtctg ttattacaac caaaactaat tgattattaa
      361 catcgtagaa caatttcgac atggtatcac gagattggtc taactgaaat ttttattaaa
      421 tgctgccata acaattcaac cccttgtggc attgccgaag taagttacat caaaaaatta
      481 aacagttact tctgcttaaa aatactacta aaatcccgat ggatataatt atatcctatt
      541 gcctgaactt tgtctgacat tacctgctga ttagctaata acatctctgc acgctcaccc
      601 atcatcaatt ttaaaacacc tgaaggaata tgcaccagaa cacctggcat tttctgtgag
      661 tttcttattt gttgtatcat atctttggcc atcaatggac atggtgctac gccattatag
      721 atgccattta gctgattatt tgacagtaaa taaaaaaaca aaccacacaa atcattaatt
      781 gaaatccaac tctgccactg attgccgttt ccaaaaacag gtgctataaa aaacctaaaa
      841 gatttcaata tttctttcag cataccaccc tgcggactca tcaccaaacc aattcttaca
      901 atacaggttc ttatgcctct ctgttcaaac atcaagtgtt caagttccca ttgatgacat
      961 agctttgcta agaaggtatc tgcaggcaag gtgttttctg tcagattaat gccttcggga
     1021 taaacaccaa tggcagatgc accaatatat gcagtaacct gatttttgtg tttgtaaact
     1081 aaatcaaaca atagtttggt tgcatttatc ctgctgttgt aaatttctgt tttacgttta
     1141 tctgaccaag gcctatctac aataccatca cctgccagat taataattgc atctacgcct
     1201 tccagacaag catcatcaat caaattcgac tctgtactcc aataaaatgt ttgtgcccct
     1261 gaaactgctt ttttggtcct gcttaatatg tgcacttcca ttccattctg aatgaacaac
     1321 tgtgtgagct gtttgccaat gaaaccactg cctccggcaa tgagtatgcg cttgcaagaa
     1381 ttcatattgc tttcaacaaa taaaataata aatttgtttg gacacaataa gaaggatatc
     1441 tacattgttt tcatcgtaat aaaaacactt ttggccctta agtaagctgt gtagaatagt
     1501 cgtgaatagg tttagtgcac attgccaaca tcacattcac cttatagaaa taaaatttat
     1561 ctatttttga aagcccttta aaattcaaca aaggcagttt acatttttaa cctcaatatt
     1621 atggatcgtt ttttcggaat ttttggtttc ctctttattc tgggtattgc ctacctgatg
     1681 tctaacaata aaaaagcaat taatttacga ttggtactaa gtggattggg gttacaattg
     1741 cttctggcat tttttattct gaagaccagt atcggacaaa tgttgtttca caaaatagcc
     1801 gatttcatta ctaaaatatt agactatgca ttgagtggtg gtgaatttgt atttggtgta
     1861 ttaatcaaga aagaaactct tgcggaagtg tttggaccgg ccagcagttt tatctttatg
     1921 tttcagataa tgccaaccat aatttttgta gccgttttag taagcattgc ctatcatatt
     1981 ggtttgatgc aacgcattgt ttctgttttt gccaaaggca tgaaggcaat aatgaatgtg
     2041 agtggtgccg aggcattgag taatgtatcg tcagcctttg tgggtcaggt tgaagcacag
     2101 attttaataa agccctacat cagtaaaatg accatgagcg aattgcttgc ctcaatgaca
     2161 ggcagtatgg cctgcattgc cggtggtgta atggctgttt acatttctat gggtgttccg
     2221 gcagaatatc ttcttgctgc aagtattatg gctgcaccgg cagcattggt aatttctaaa
     2281 attgtttatc ccgaaacaga agaatcagaa acaaaaggca ccgttaagct tgaaataaaa
     2341 aagacgcaca gcaatctgat ggatgcaatc tctcatggtg ccagcgatgg actaaaagtt
     2401 tcattgaatg taattgccat gctcattggt gtcattgcat taattgcgct gattgatgca
     2461 acacttggtt tctgcgggcg acatcttgct gattggggtt taagtctgag tgcaatcaac
     2521 attgatttag ccaaacttag tctcaaagaa attctgggtg ctgtattttc aatttttgct
     2581 tatctgatgg gtgtgccttc tcaggatgtg catatagcgg gtcagctttt gggaacaaag
     2641 cttgccgtga atgaatttgt tgcctatgta gattttacag cagcaatgaa aacaatgtcg
     2701 ccaaaggcaa taacaatttt aagtattgca ctttgcggat ttgcaaactt cagctctgtg
     2761 gctattcagg taggtggcat tggtgaactt gctccatcgc gcagagccga cctggcaaaa
     2821 ctaggtttaa aagcattggt atgcggaaca ttagcatctt atctttctgc aacattggca
     2881 ggtattctga tgtaacaaca aaatcatatt tcagttgttg taattatgga atagcaattt
     2941 agcttgcatc ttttttaaaa acaacaatta tgttcagact tacttttatt cttctgcttt
     3001 tatccataac atgcaaggca caacaggatg cacaaataac aggtgtaatt tctgataatg
     3061 acaacaagga gccattaccc ggtgtaatga tcagctatgg aaccaatgaa cttacacaag
     3121 cagtttttac agacgataaa ggcagcttta aaataagacc acttgcaccc ggcagttaca
     3181 acatccgttt ttctatatta ggatatgaaa cattaagtac tataggtgtc agagtttctg
     3241 acggagaaac tgttgtactt aacaaaactt taaacttcag caatacgctg cccgttgttc
     3301 gaaaagaaga atacagaatt ccactgatgc cacgtaatcc aaacacacca ttagtcatca
     3361 gctatgaaga cataaagtat gccgttgaca aaaacattaa tgcattggct gcttctacag
     3421 caaaagtttt tcaggccgat gccggagagc caataagtat gtccggctcg cgatatggta
     3481 ctgtaaaata tattgttgac ggagtaatta ctgaaaatga acctaagttg ccgggatgtg
     3541 ctattgagca gattagcatt tacaacagca gtattcctgc ttgctatggc gatgctacag
     3601 gcggtataat tgttattact tcaaaaagtt atcgtttctg aaaaactatg ttgtaatttc
     3661 aggcttgcat gttgttttaa agcgatgtaa aagtaaaacc atcataccaa acaatagaaa
     3721 tgctgttgcc tgatgaacaa cacctaacca aaccggcacg ccataaagca aagtaaaaac
     3781 accaagtatg aattgaagca ccactaaagc aggtaataac caaagtgtac tcagtacttc
     3841 aggatatagt aacttattca atcttgcagt gtttaaattc tttgctgtga aatacagaac
     3901 cagaaacaaa atcaccactg cataagcaac ataacgatga ataaactgta ctacaggcat
     3961 ggcatcaaac aatgctgctg cacccaattt ttgcaaactc attccaactg catcagaaat
     4021 ccagtgatca tccatttttg gaaatgaatt aaaaattctt ccggctttaa gtccggcaac
     4081 aaaagcacca taaattatct gtacgcataa aacaatcatg gttatccatg aaagtttata
     4141 aagttttttc agatgcaaat catattctct gcgttgcgga aaaatataat cgagtgccac
     4201 ataaaaagta gcaccgaagg tgatgaatgc agaaatgaga tgaattgcca ggcgataatg
     4261 actcacagaa gtacgttccg taagtccgct tttaaccata aaccaaccca aaaaaccttg
     4321 cagtccaccc agaaaaaaca acaacagcat tttgcgcagg cgctcattgt taaactgttt
     4381 ttttatccag aaaaatagga aagggaaaaa gaaaaccatt ccaattacac gacctaacaa
     4441 acggtgaaaa tattcccaga aataaatttg tttgaaatct tctaagctaa aattgtaatt
     4501 aagcaattga tactgtggaa tagctttata actgtcaaac tcttccatcc attctttctc
     4561 attgagtggt ggaatggttc catgaatgag tttccattct gtaatagaca atccgctacc
     4621 ggttagtctg gttatgccac cgataacaac cattgcagca ataagtaaac atcctgtcag
     4681 caaccaaatg ataattgcct ttctgtattt taaattataa atttcagtac tcattttaca
     4741 ctttctctta aaacaaactg agctgtgttg cgactacgtt gctccaacaa tatttctttg
     4801 cctgatgtaa tggcatcaat agtgttttga taataatgcc ttatggtata aagcatcagg
     4861 tcttcattat atcttatttt ataatccttt tgcaatgctt ctatcaatgc agggatttta
     4921 aaactatcgt tatcaacgca aacagaaaag ctgatggcgg aattctgcat aaggttaatt
     4981 tttactccat gtccggcaaa caaagcaaaa atacgcgaca gattatcttc tgcaataaat
     5041 gaaaaatctt tagctgcaat actgataagc acctgattgt ttttaaaaat ataagaaggt
     5101 atcagtggct ttgtctgaac attggtgcgg attgccgtac cattagcttc aggtttgaga
     5161 aaagatttta cataaagcgg tatgttttta ttttcaagcg gttttattgt cttggggtga
     5221 atgactgtag ctccataata agcaagctca atggcatcat gataactaat ctgatcaagt
     5281 ttttgtgcat ccttataaaa tttcggatcg gcattcaaca caccagggac atctttccag
     5341 atatacatgg cttctgcatc gagaagataa gcaaaaattg ctgctgtata atcagagcct
     5401 tcgcgaccta atgttgttgt ataattttct gatgtgccgc caataaatcc ctgagtaatg
     5461 ataacatcaa cccggttaaa aacaggtact acttgtttct gcaccagcat ttcacttttt
     5521 tgccagtcaa cttttgcttc gcgataggtg ttatcggtca tcagtacatc gcgcacatcg
     5581 agccagatgt ttgaacagcc ttgctgttcc agataagcac tgacaatgca agtagaaatc
     5641 aactcaccaa gagatactat ctgatcgtaa acaaaactat aagtatgctg tggtttttca
     5701 tcaagcatcc agtcggcttc aacaaaaaag ttgtttacct tatcaaaaac aggatttgtt
     5761 ttttctgaaa acaattcctc cataatttgc tgatgatagt ctcttacttc ctttaatgat
     5821 gtgtgtgcat taccttttga aaaaaaataa tcgtttacca ctttttctaa tgcattggtc
     5881 gtttttccca ttgcagaaat tacaacaagc aacttctcgc ctttgaatcc tttcagaatt
     5941 tcaactgcat tttttacaga agtagcatct ttaaccgatg ctccgccaaa cttaaaaacc
     6001 ttcattacaa tacctgaaaa ataaaacaac aaaaatgcaa gttatcatct tatattcctg
     6061 atgtcagaat aaaaaaatca aaaagttata ccgaagtttt tgtacttaac ctgttattca
     6121 ctatgattgt tctaatttaa cacactcaaa atggacatac tgattattat tatttctgca
     6181 ttattgcttt tagccggcat tgcaggatgc atacttcctg tgctgcccgg attacccatt
     6241 gcttttggcg gactattatt agctgactgg cattttaatt tctatacagg acgttttctg
     6301 tggattatgt ttgccatggt catcataatt tctattcttg attattatct ccctatttgg
     6361 acagctaaac gatttggtgc tacacgttat ggagtgattg gaagtttcat tggtatgtta
     6421 ctaggaattt ttctcactcc tgttggaatg ataatgggaa caattattgg cgctatcgtt
     6481 ggcgattttc ttgcaggaaa aaatacaaga gatgccttac ggtcgggatt ggcaacagta
     6541 ggcggaacct ttatttcaac aggaataaaa ttagttggtg ccggaattat gacctggcat
     6601 ttcgtcaagt caaccatatc atattatgcc gattagcagt agagttttat atttgcataa
     6661 ataaaacatt aattttgaaa gcaataatta agggtcagga aattaatctc aacttcgatt
     6721 cgaagaaaaa agttttaaca caaaacaatc agcaagttga tattgacatt aaaaaaatat
     6781 ctgagtcgtc attaagtatt ctgctacaaa acaaatcgta taaggctgaa ttacttgaag
     6841 caaattacac tgaaaagcaa tttaaaataa gagttaacgg caatgtttat caggttgcac
     6901 tgaaagataa atacgatgag ttgctacagc aattgggcat ggataaaatg tttgctcaaa
     6961 aagtaaacga cctaaaggct ccaatgccag gtttggtcct cgacatttta gtttctgaag
     7021 gacaaacaat tcaaaaaggt gatagcttgt tggtgcttga agctatgaag atggaaaaca
     7081 acctgaaagc cattaatgat gcagttgtaa aaaaaataaa tgtatctaag ggtgataaag
     7141 ttgaaaagaa tacggtatta attgaattat cttaatcata aagaaaatga aaaacaggag
     7201 agattttata aaaacttctg cattggcagc aggaacgctg gcattgtacg gaatcggcag
     7261 caaggttgcc tttgcatcag aaaacaacga agacaactta tttaaattac ctgatttgga
     7321 atatggcttc gaagcattag agccatatat tgatacgcta acgatggaaa tccattacac
     7381 caagcatcat cagggatatg taaacaatct taacaaagca gtcgttgcag aaaaattaca
     7441 aggcgtgtca ttaaatgaat tgttaggcaa agtatcaaaa cattctgctg caattcgcaa
     7501 caatgccggt ggacattaca atcattcttt tttctggagg ttgatgcgtc cgggaggatc
     7561 taaaacacca accaatgctt tagccgaagc catcacaaaa gattttggca acattgacaa
     7621 tctgcaaaaa gtattttctg atgctgcaaa aaacagattt ggttctggat gggcttggct
     7681 tattaaatca accgatggca aactaagtgt tacatctact ccaaatcagg acaacacgct
     7741 gatggatatt gctgaagtta aaggcactcc tgttttggga attgatgttt gggagcatgc
     7801 atattatctg cactatcaga ataaaagagc agattatgta aatgcctggt ggaatctgat
     7861 caactgggat gtagtgttgg aaaactataa tgcctgagca tttagtttgc ggccaaatca
     7921 gactttatag catcttttaa aacaatcatc acctgacggt caaaaatcat atcactgtca
     7981 gactgctttg tattaaaata cgatgttaat ttatcctgac gttcaaaagg aagcaactga
     8041 cgtatttggc ttaacaaata acttcttctc tctttcaaaa tcattaactt ggtaagcaac
     8101 tgctccggat taatattttt taatgagtca ggaagaaaat gtgcatctac atttttaaga
     8161 ttaccgtttc ttgattttaa gaaatcaact aaatcccaat cgctctgttt tccctgaaag
     8221 cgatcggaaa ttttatgata aagtaaatct tcaaaagttg aatgacgtgc attaagtgca
     8281 ttcttctcat tacttaccat tgccttaaac ttatcacgac ctgctttacc ataatagata
     8341 taagttttat tgagttcggc tgccagcatc tgaagacggt taaaatctgt aaccgttgca
     8401 tagtcaggaa ggcgtttatg cactttaaca tcaatagact ttccaccggt aatttcagaa
     8461 attttatacc agccactaat ttctttagaa cgtaatgaac tgtagcaata caatgaattt
     8521 actgctattc ctttttcttt tgccagatta catgattcta caacatcaaa tgcacctaaa
     8581 aacacagaac catttccggt aagaaaaatt tgcttcacag catctttttc atgagaccaa
     8641 ctcagcagat ctaatgaagc acgaatagcc gcacctacat actgatcgcc tttttcaata
     8701 ttgggcttca gtttgtaaag ctctgtagct ataaagtcaa tgtttgatgt aaaaggagaa
     8761 ataactttaa cgaactgatt ttttcctcca aaacttgggc gcgagtagcc aacaacagca
     8821 aaacgaatat cagtacccgg ataaagtcgg gtaagttcat tattcattcc ccatatgtta
     8881 tcacgaacat catcaatcag cccattggtg cttccactga ggtcaagtgt aaagactatg
     8941 tcaacaggtt gattcttagc tgaaacctca gcttgaggta gcaaccatgc caaaaatagg
     9001 gctataattg ctagttttaa tcttaacagg ttcattgttt attgtggatt tattgtggtt
     9061 tttcaaaatt agatatttta ttgttttgtg gtttagtctc taatattggt acttttgaac
     9121 actttattgt ttatgaacga aacaaatatt cagaaaatca agcataagtg gggtgaactt
     9181 ttacggcaat tggaagaaac ctttggtgaa aagcccgatt tacagggtat gattttcctt
     9241 attggagtac aggaactggg aaaaggatat cagaacttta ctaaggaaga aaagcaggat
     9301 ttaatgcaca ttgccacatg caaactcctt agctatgaag gattttacaa gcttataggc
     9361 gttgatgccg atgggtggcc acagtatgaa aaggctaaaa caatgcctca aatgccatta
     9421 aaagagcagg atttattgct aaaaatcaat attattcgct attttgagga agatggatat
     9481 gaacttgaaa aaagtgaagt tatttgataa ttactgtacg gtaaacagtt tttgattgct
     9541 ccaaacgtat ataatcttta ccaagtttag gtcccttgct ggcttcaata tttaaaactg
     9601 cctccaactt aaactcaaaa gaggctttac catagccatc agtaacgcct tcctgataca
     9661 cattagcctg taccccggta gttgggttaa caactgcatc ttgtcttaga atcacggttg
     9721 cacctttaac agctactcca gcactatcgg taatggttat gatagcttcg caaggtccct
     9781 cctttttaca ggaaaaagta aaaagaactg tcattactat caatgaagtt aaaactaaat
     9841 gagttttgcg taaactactt ttcatgcgaa tggaaattta ttattcttgt acctgttttt
     9901 acgaacggaa tcgtaaaatg ttttatcagc tcaaaagtaa ttaaaaatag aaatgaaaaa
     9961 aatcatctgt attattgcaa caatccttcc cctgattgca ctgtcacaaa ccgataaaag
    10021 tcaggctaaa attcagtcag aaaaaagtca aaacaaagaa accatggtgg aaataacaac
    10081 caatttaggt acaatgcgtt ttaaactcta tagtgaaacg cccttgcatc gcgacaattt
    10141 cattaaactt gccaatgaag gttttttcga cagcacgttg tttcatcgtg ttatcaaaaa
    10201 ctttatggtt cagggtggcg atccgcagtc taaaaatgca gctgccggtg ctatgttggg
    10261 taatggtggc ccgggataca ctatacctgc tgaatttaat actgcattat atcacaaaaa
    10321 aggtgcattg gcagctgcac gtcttggcga tcaggtaaat cctcaaaaag catcttcagg
    10381 aagtcagttt tatattgtgc agggaaaacc tgcagccgat aacgagctga ctatgatgga
    10441 aaatgccaaa cacatgaaat atacgcctga acaacgcaaa gaatacacaa ccgttggtgg
    10501 tacaccgttt ttagatatgg actacaccgt ttttggtgaa ctcgtttccg gcatggacgt
    10561 ccttgataag attgcagcag aggcaaccaa tcctcaaaac cgacctttgc aagatatcag
    10621 aatgactgtt aaggtgataa aataattttt gtatcatttc attaaataaa aaatcaggtt
    10681 cagtgaacct gattttttat ttttaagtat aaatcatcaa ctcaatttaa aattatttac
    10741 gttattcgtt aaggttaaat ggcgaagaaa caatcattag gaaaaagaat aattatagtt
    10801 atactattat tttttggtat tgcagtgttg gcattttttt accgcaatca gataatacgt
    10861 tatggctata aagcatggcg cctggaaaaa agattgttta ataaagaaaa ccatgcagca
    10921 acaaaaataa gttttccaaa aggctatgct gttcatggta ttgatgtttc acgctggcag
    10981 acagacatta actggagcca attaaaagct gccgacctta atggagatac tattaatttc
    11041 agttttgcat ttattaaagc taccgaagga attttacttg aagacccttc ttttattgat
    11101 aactgggcag gtgcacaaaa gaataatatt attcgcggag cttatcatta ttttcttccg
    11161 aatgctgatg tatctttaca agcaaaaaat tttatttcaa gtgtaaaact aaagagtggt
    11221 gatttgccac ccgtaattga tgtagaagag aaaggtaatc ttacaaaaaa agccttggta
    11281 aaaaaattaa aacaatttat aactattatc gaaaaacaat ataaagttaa gcctattatt
    11341 tattccaata tcagttttat tgaagattat ttggcagatg attttcctga ctacaacttt
    11401 tggattgcac actattacga agatgaatta aaaattgaga agtcggtaaa atggcttttc
    11461 tggcaacact ccgaccgtgt ttccattccg ggttcaagct catcttatga tgccaatgta
    11521 tttaatggaa gtaaaaatga tttaaaaaaa ctgttgatta agtaaatact cacaagaact
    11581 taggttcaat ttttctctaa aaattatgaa gcaataacct gaattccgat gcaagcacaa
    11641 aaccattttc tttgcacatc aatcctgatg gaatgaatca aatcaaacaa aaaataattc
    11701 tggcttcacg ttcaccacgc aggcaacagc ttttgcaaat gatgggcatt gcatttgaag
    11761 taaaaacaaa agaaactgat gaagattatc ctgaacattt aaagaacgaa cagatagtct
    11821 tacatctcgc ttcaaaaaaa gctattgcct tcgaaaatga gattcaagaa aatgaattgc
    11881 tcatcagcgc tgacaccatt gttgttcatc agaataaagt actgaataaa ccggcagatt
    11941 acaatgaggc cattgaaatg ctcaccactc ttaacggcag catgcatgaa gtttatacag
    12001 gagtttgtat tgcttccatt cacaaaaaag aattactcta tgataagagc gaagtttatt
    12061 ttcgcaacta cactgttgaa gacatgcatt attacattac caattttaaa ccttatgata
    12121 aggcaggttc ttacggaatt caggattggt ttggtctgac gtgtatagaa aaaattaacg
    12181 gatgctttta taatgttatg ggcttgccaa caagaaaggt ttatcagcaa ttaataaatt
    12241 tttagttcat gcgaaaggct tatctgcaaa tgcacatctc cattttattg tggggattta
    12301 ccggaatatt tggtaaggca attgggatga atgccatcat gattgtatgg tacagaatga
    12361 tcatcagtgc tgttgcactg ataccattta tgataaaaca taaacagttt atcttacctg
    12421 atagaaaaag actggcatca atcatcataa caggttttgt agtttgtctg cattggctta
    12481 ctttctatgc atcaataaaa gcatccaatg tttcaattgc actcagttgt tttgcttcgg
    12541 tgtcactatt ttcagcatta ttagaaccac tattttaccg caagaaaata gataaacaaa
    12601 acatcatttt agcgttgttt gtacttgttg gcatctatat catttttgct tttcagcagt
    12661 tatatgctat gggaattatt ctggcactaa tcagtgcgtt gctcggtgca ttatttacta
    12721 ttcttaataa aatatttgta agtaatgatg agcctgcacc ggtaacattt atagaattaa
    12781 tcagcggatt tgtattttta tctgttcttc ttccactcat cctacctgct tttaatctta
    12841 gttttcaaat tccatcaaag attgattggg tgtggctgct attgctgagt gtagtttgta
    12901 cttcagttgc ttttacgctt tcattggagg cattaaaaaa ggtgagcgca ttcaccatga
    12961 acctctctgt aaatcttgaa ccgctttaca gcattgttct ggcaataatc ttatttaaag
    13021 aaaatgaatt gctcaataac ggtttctaca ttggcgcagt cattgtcatc agttctgttt
    13081 ttattcacag tctgatgcaa taccgggtca gagataaatc aatcaccgag caaggctaaa
    13141 gcctttcgta aagaaataaa catctgttca atatcttcca tacgagctgt accaatagaa
    13201 agacgaaacc aatttgaagt tgtttctgca ccaaatgcag aaaatggcac taatgctacc
    13261 gtggcatagt taatcagaaa cgatgtgatg tcgtttgaat tgtttatccg tttaccatca
    13321 ggcgtcactt tatttaaaat atcaaactga acggtgaggt atatggcagc ttgcggatca
    13381 atggcattga caggcaaacc atcttttttc attttcaaaa agccatcata aaatgcactc
    13441 agtctttttt ctacttcagc tttgtaatga cttaaaaaaa catcaacctc attttcctga
    13501 gttagaaaat gtgctgttgc tacctgttct gcctttggcg accatgcgcc tacatgcgaa
    13561 agaatggatt tcattttatc aataatgcct gccggaccaa aagcccaacc tacacgtaaa
    13621 cctgtggcag aaaaagcttt ggaaagacca tcaataaaaa ttgtgtaagg tcgcatttca
    13681 ggaagtaaag aaacaggatc aacatgtttt gttttcccaa aagttaatgc catataaatc
    13741 tgatcgtaca tcagatacaa tggtttctta ccttctcttc ttttgttttc ctgtaatatc
    13801 aattcacaaa tttccatcaa ttgcgcactg ctaaaaactg tacctgtagg attaagcggt
    13861 gagcaaaggg caaccaatgc tgcagatgac agatgcgact tgagttccga tgcaagcggc
    13921 ataaaattat tattgggctg tgtttctaca gcaacagctt ctgcctttgc taaatggcaa
    13981 tagtgattat tgttccatga tggtataggg aaaactactt tttcaccggg gtttacaact
    14041 gttttgtaca atgcataaat gagcggtcgt gcccctccgg aaatcaaaat ctcattacta
    14101 ctgtattgca acttttgcag tttattgata tagcctgcaa cagcactacg cagctccggc
    14161 tgaccgttgg caacaggata attggtgtat ccatcctgat aggctttaat aatttcattc
    14221 ttcagtgagt cgggaatagg aaatatagca gggtcataat cgccaatggt cagattaaaa
    14281 atcttaatac ctttttttat ttgctcgttt acttcgccag cgagttttat gatttccgaa
    14341 ccaattaaag tttcggccat atcagaggct gtatatgccc ctttttctaa tgtagcttgc
    14401 atcatttcat tcaaaaattt gctccgaaag tattcagaaa atatgagtta acaatgcaag
    14461 tttactcatt taccgggata ataaaatatc atccgaaaaa tgggtttgtt tttttatctt
    14521 tgcacacttc tgaaaaaaaa ttcaattaat aaaaatacca tagagaatgg aatttgcagc
    14581 tacagaaaat cagcagatga ttgcacaaac ggtaaaagat tttaccgaaa aacacatcag
    14641 acccaatgtg atgacatggg acgaagcaca ggaatttcct ttagatgttt tcaaaaaaat
    14701 gggcgatatc gggctgatgg gcatattggt accggagcaa tatcaaggtg caggattagg
    14761 ttattttgaa tacgttaatg ccattgtaga agtagcaaga gtttgcggat ctgtaggctt
    14821 atcaatggct gcgcataact cactatgcac aggacacatt ttagcttttg gaaacgaaga
    14881 acagaaaaag aaatggttac ctaaattagc taccgggcaa tggattggcg catggggact
    14941 aacagaagcc aatacaggta gtgatgccat gcgtatgcaa tgtgtggcca agcaagatgg
    15001 cgacagctgg attattaatg gtgcaaaaaa ctggattact catggcaaaa gcggtgatgt
    15061 ggcagtagtg cttgcacgca ccggtgaatt gctcgactcc aatggcatca cagcatttgt
    15121 ggttgagcgc ggaacaccgg gatttaaagc aggtaaaaaa gaaaataaat taggaatgcg
    15181 atgcagcgaa actgctgaaa tgatttttga ggactgcaga gttccggcct caaatatttt
    15241 aggcaaagtt ggcgatggct ttaaacaggc aatgaagata ttggatggcg gacgtatttc
    15301 tattgctgca ttatcattag gtattgcaaa gggtgccttt gatgcatcgg ttaaatatgc
    15361 aaaagaacga caacagttcg ggcagcccat tgctaacttt caggcaatag gttttaagtt
    15421 ggccgacatg gcaacaaaga ttgaagcatc agagctttta atattacaag cagctgattt
    15481 aaaaaaccga ggtgaaaaag ttacccgtca gtcggcttat gcaaaatatt acgcttctga
    15541 agtggccgtt tatgtaagca cagaagcggt gcagattttt ggcggttatg gttataccaa
    15601 agactttccg gtagaaaaat tttatcgcga cagtaaactt tgcaccattg gagaaggcac
    15661 ttccgaaatt caaaaattag ttatagcaag agaattactg aaataaaacc tgacttaaca
    15721 aaaaaggctg cctacttttg agcagccttt tttgttttga tgattcttct tctatcagaa
    15781 caaccaacct aattttattt tagccgtaac attggggcca aagcctgaat cttttccttg
    15841 tttcattgaa ggattactct ttgttgtagt agtttgtgct gcagtaatag tacttacccc
    15901 attagcatca ggcacagatt ctgtccatgt attttttaca tctaagtagt cagattttaa
    15961 atcatcatat ttagtactgt aaaaaccaaa accaaattca gcacctaatg aaagattttt
    16021 actgaaatag taatccatac ctgcaatcag attaacacca aaagttttgc tgcctgatga
    16081 accacttatt gattttgtag tccagggtgc agaaacaatt actgttgttg atgtagtatc
    16141 ataaaacgtt ggtgacgtat aatctgttgg taatatagta tcgcgtgtga attttgtttt
    16201 tgaactgctg aaactaatct ctgcaccaat atatggtgac agcctgtctg tgccttcaaa
    16261 atgcttttca atacccggac ggaaactaaa gccagatgtt ttgcttacca aatctgcttg
    16321 tggattcata cctgtagtta attgaatata accacctcct ccttcagcat gaaacacggt
    16381 tgttgttgtc gggaatgcaa gtgtatcatc tgcctgcata ataacatctg tactttttga
    16441 actgctgata gaaatactca atcttgctgc aagttttcca tcttttaaga atctgcgata
    16501 agtaattcct tcattcaatg aaagtggtgt acttccaaat ggtgcaaaca ataattggag
    16561 gttattttct ccacctgctt gtttgtaagg ttgagcctca acggctgtca gacacaggaa
    16621 catgcctgtt gcaattaaca ttagtctttt cattttattt ttcagttttt gtttcgtgta
    16681 aaaatatccc ctgaaacact gttttaaaat tcaaatcatc agaattataa acatttcaat
    16741 atgaataact caaaaatgtc tattcaaatc aaggtgctac aatttgttgt agtgcaggat
    16801 attcattaat ccttgatgtt tggttaaaac cactctgtac agctttatta aagactgcca
    16861 ttgccttttc gggttgcttg ttgttggcat gataaactgc cgaaagaaac catgcctctg
    16921 aattattggg ttcaactgtc tgataaattt gtagaacttt atctgactcc ggagcatttt
    16981 cattgactaa ttgtttggca aaagaataac aggcaatacc aataaatcct aacaggcggt
    17041 tactcatggc tgccaacatt ttgtcattac tgtttttagc tgcaattttt aaatcagcaa
    17101 tttctttttg ccaccatgat atgttctgat ttgaaaaagc atcgcgcaac atcttctgca
    17161 tttctttttc ctgatattgc aagttctgac ttcgcatacg ccacttacgt aggctctcgg
    17221 agttttcaat ttctttaagt ttacttttaa ggcttgctac atctgtcagt ccatcaaagc
    17281 acacaactcc aaactgcaac tgcttaaatt gtttatccac agaagattta ttttgtaaac
    17341 tattaatttt ctttagccag gtttgataat aattttctat aactgttttc tgtatctgca
    17401 atttattctg cttcaccatt tgcatcagag taaagagcat ggcatcatac atatcacttt
    17461 tatccggcca ttgatgtgta ctgtccgttt caataaatgc atggtgtgtt acgtcaacag
    17521 atttttctaa agcaaccatt tcatgataat taaaatcatt gatgcctgct actgaaacca
    17581 cactcatatt cttatcaaac ttaccattct cgaacaatgc tcccgaacat gctatcaatc
    17641 ctgaaaaaaa atgttgattc attgcaagat tacaagccac tttacttcct cctgaaaagc
    17701 ctgcaattat ctgctgctgc atgtttatcg gcaacctgtt ctgacaatca gaaaaaagaa
    17761 cattggcaat ttgctgcgac tgttcgaaag gcattccgtt ttctacactg ttgcaaccaa
    17821 ccataatgaa cttaaaagca tcagcaactg atttgtattt ttctaagggc aaatttccat
    17881 ctgcatgagg atcgaaaaaa tatactaccg gaggtaatgt agtatcatta tattctgatg
    17941 gtacatacac agaaaaagac acattactat tactctctga cttgatattg gaaagaattt
    18001 ttccgggttc taattttatt ttggtaaaat gtgtctgtac tacagttgaa gaatcaactt
    18061 gttgcacagc ttctttcttt tcaggcgctg atgcacatga taagaaaagg gaacaaacaa
    18121 ttaaaaaatt ccaaaaaatt atcaatctat ttttcatcac catcattatt aaaccaacct
    18181 gaatacatta cataattatt tgcaatacgc tcattcaaat ctttaaaatg ctcggggctt
    18241 acttctttaa ctttcttggc aggaactccg gcatagatac aaccagattc tacaactgta
    18301 ttttccagca caacagaacc tgctgcaatt attgcatttg atttaatgat tgcatgatcc
    18361 attacaattg cacccatacc aatgagcaca ttatcttcaa gtgtacagcc atgtacaatg
    18421 gcattatgac caatggaaac attatttcca attaccgttg gagctttctg ataggtacaa
    18481 tgaataatag caccgtcctg aatattaact ttattgccaa ctgtaattga atgtacatca
    18541 cctcttacca cagcattaaa ccatacacta caatcatcgc ccatcaccac ctgcccaact
    18601 attgtagcat tttcagcagc ataaaaattc tttccaaact taggcataac gccattaaca
    18661 ggttttataa gtgccatgaa ttatttttct tcaaaactac aactaccatt caataaaaag
    18721 aaattgtctg ttaccaaaaa actattcttt actgatatga ccttcccttc cattgataat
    18781 gatcagacaa acttaaaacg ccaacaacaa aagtatagat aggaaacaac aactgcatta
    18841 acacaaatgc accgggataa aatttctgat taagctttga ttttgcattc tgaataaaaa
    18901 taaaatcgca gatccatttt actgcaattg ttattgctga cactaaccaa agctgattta
    18961 acggcaaaaa tattactcca ggaatagcag catattgcag tatagaaaac atggtaagaa
    19021 acaaaccggt tatgataata cattgctgtt tataatattt caattttgat gcccatctta
    19081 cccgttggtt tatcaactct gataatccgg attgagcatc agacattaca gtaactttat
    19141 cggaagctaa agcatgaata ctttgattat tttttgcaaa caatgacaac ataaaaaaag
    19201 tatcgtcacc tgaggctata tcacttcgca tttcagcatt gtttatttgt aaataggttt
    19261 ccttcttaaa aacaagattg gcgccattgc acattaatgg ttttccaata gccaatgaac
    19321 caaaagttaa agctgccaag cctaaagact ctgtttgttg catttttgat aagacccctt
    19381 ttccctgaat gacaacaggc aatatcaaca tatcaggctt tgataaatta aattcgttgt
    19441 ttattccttt aagccaatct ggattgaaaa cacaatctgc atcagttgta attattactt
    19501 ctgctttact tatttttatt ccttctgcta tagctgactt tttgcctttt gaattctgaa
    19561 gtgaaattac agtaccattt aaattgtata tattaagtaa ctgtgttgcc aatgtcgcag
    19621 ttccatcagt agagtgatca tcaacaataa taatttcaaa atcagctttc ttatagttta
    19681 agtctgtcag cgaatgcagc aatcgttcca tgttggcttg ctcattacgt actgcaataa
    19741 ctattgaaaa aaagttgttg atttgatgcg gttgaaccgg ttgtttttta ttagctattg
    19801 tattaaagcc aagcatccat ttgaaagaca aaaaaacata caaggccgga atgattaaca
    19861 aaaaaagcat aggcaaatgt aaggatagga aaaattaaat cgtcacttta atatatggat
    19921 aaaaaaaaac cgcattgctg cggttttaaa atttataaaa ggaatgttac ccgttgagtg
    19981 cttctgcacc tgccacaatc tcaagaattt cgtttgtgat agctgcttgt ctggctttat
    20041 tgtattgcag acgaaggtct ttaattaatt cacctgcatt atcggtggcc ttattcatgg
    20101 cagtcattcg ggcaccgtgt tcacttgcat gcgaatcaag cacagcttta aacaattgtg
    20161 tttttacagc tgtgggcaac aggtcgctta caattgattc tttattaggc tcaaagatat
    20221 aatcactaac tgttttgctt ttttcttgtg acgcaggagg gataattggc aacaattgtt
    20281 ctgtctgcaa tatttgtact gcagcatttt taaactgatt gtaaactaaa attaccctat
    20341 cccacttttc ttttgcaaac tcatccataa tgcgttcagc gattactgca gtgtttgcaa
    20401 aattcaatgc attgtacaaa ctgttttcat taccgaaata aacattttga ttctttgcaa
    20461 aaaagtctgc tgacttttta ccgattgcaa gaactttcac tttgccttct ttagcttgtt
    20521 tctgatatgt ttcattaatc aattttgatg ttgccttaat taccgatgag ttaaatgcac
    20581 cggcaagacc acggtttgaa gaaattgaaa cgatgaggat attatttgca ggacgcacct
    20641 tggtaaatgg gcttccgtct gatgacaaac tttgtgaaag attttcaagt atctctctca
    20701 aattagcagc ataaggacgc atctgcataa ttgcatcctg tgctctgcgc agtttagcag
    20761 cagacaccat tttcattgcc ttggtaattt gctgagttga atttaccgaa gcaatgcgca
    20821 gtcgtacttc ttttaaattg gccatttatt aatttagaat tatgaattaa gaaatcagaa
    20881 tattattgag ttcttagttc tactttttta cgaatatttt ggtgccaagt ctgcagcaac
    20941 tttttcaagt acagaagtta tactatcatc aaattcacct tttttcaatc tatcaagaat
    21001 aggcttttgt gttgcttcaa gcagactaag ataattagtt tcaaactctc ttactttctt
    21061 tacgtcaaca ttacgtaaca aaccttttgt tccaagataa atgatggcaa cctgctgttc
    21121 aacacgcaat ggtgagtact gtccttgctt aagaatctca acgttacggg cacctttatc
    21181 caatacgttt ttagtagcag catcaaggtc agaaccaaat tttgcaaaag cttctaactc
    21241 gcggtactgt gcctgatcaa gctttaatgt tcctgcaact tttttcattg atttaatctg
    21301 tgcattacct ccaacacgag atactgagat acctacgttg atggcaggac gaacaccact
    21361 gttaaacaaa ttagcctcaa ggaatatctg accatcagta atagaaatta cgttggttgg
    21421 aatatatgct gaaacgtcac ctgcctgtgt ttcgatgatt ggcagtgctg ttaaagatcc
    21481 accacctttt acttttcctt taagtgaatc aggaaggtcg ttcatttgtt gagcaatgct
    21541 gtctgatgca ttgatttttg cagcacgctc aagcaggcgg ctatgcaaat aaaatacatc
    21601 accgggatat gcttcacgtc cgggaggtct tctcaacaac aaagacacct cacgatatgc
    21661 aacagcttgt tttgacaaat catcataaac aatcaatgca ggacggccgg tatcacggaa
    21721 atattcacca atagcagcac ctgcaaatgg tgcataaaac tgcattggtg caggatctga
    21781 cgcagatgcc gaaacaatta ccgtatatgg catggctcca cgctcttcta atgtttttac
    21841 aatgtttgca acagtagagc ctttttgtcc tatggcaaca tagatacaat atacaggttt
    21901 acctgcatca taaaattctt tttggttaat gatggtgtca atacacacag ctgttttacc
    21961 tgtctgacgg tcaccgatta ccaactcacg ctgaccacgt ccgattggaa tcatggcatc
    22021 aatagctttg atacctgttt gtagtggttc gtttaccggc tgacggaaaa ttacgccagg
    22081 tgctttacgc tctaatggca tttcaaaggt ttctccttta attggacctt ttccatcaat
    22141 aggattaccc aaggtatcaa caacacgacc aagcatacct tcaccaacat ttattgatgc
    22201 aatcaagcct gtacgttttg ctgtatcgcc ttctttgata tcagtagcag taccaagcaa
    22261 tacggcacct acgttatctt cttcaaggtt aagtacaaca cctttaagtc cgttttcaaa
    22321 ttcaatcaat tcacctgact gaacattggt taaaccatat atacgggcaa taccatcacc
    22381 aacctgcaat acggtaccaa cttcctgtag gtcggaagaa gttttaaatc cggacagttg
    22441 ttgtctcaaa attgccgaaa cctcatcggg tcttatctct accattgtat ttgaatttta
    22501 aattttataa aagtttttta ataatttttc tcgtaaaggt taatctttaa ctctctgcga
    22561 atttcgcgca gtttagtttc tatactggta tcaacctgct tattattcat cttcaacaca
    22621 aacccaccaa tcaaatcctt atctattact tcaaccaatt ccactttacc ttttgcttcg
    22681 gcagaaacta tcttcattat ttcagcacgc aaactgtcat ctaacttaaa tggggttgta
    22741 acaactgctg tagctatgcc catatgagct ttgtaaagat tgataaactg acgggttata
    22801 tcttcaagat atccttctct tctttttgct acaataatat tgataaaagc ataagtaaca
    22861 tccgatactt tacctttaaa aattgaagag agaatatttt gttttttgtc ggaattgata
    22921 attggattcc ggagtaataa agcaagttca cggcttgacg aaatagtcga aaccattagc
    22981 tccatatcag cataaatttt ctgctcggtt ttttgctcta ttgccaatcc aagtagcgat
    23041 ttggcataac gtccggcaac ttttgtgtcc attgcagtat gattaattca tttttacatc
    23101 cttgagcaaa tcggcaacga gggctttttg ttttccatcg tcagcgagtt gctgcttcat
    23161 tattttctct gcaatttcaa cagaaagcaa tgccacctga ttttttagat cagtgatagc
    23221 agccattttt tcattatgaa tgccttcacg cgcaagggtc agtaagcgat cggcctctgc
    23281 agttgcttta tcgcgcgctt cgttaatgat gttatcttta gttacacgtg cttctttaag
    23341 aattttgtct ctttcctgac gcgcttctgc caacaatttt tcattgtcgg acttcaggtt
    23401 tgccatttca tcttttgctt tgatggcagc atttaacgcg tcttcaatag acttatcacg
    23461 ttcattgagt gcatttagaa ttggcttcca tgcaaatttt gtaagcagaa acagaacgat
    23521 aaggaatccc aataccatcc aaaatgttaa accaaatgcg ggttttagca gttccatgaa
    23581 tatatttttt tgtttaattt ttaatttaaa ttttctgtac aggcaaccaa tcgttacctg
    23641 cacagaaaat gctgtaatga attacttgaa tactacaagt agtccaacta ccattgcgaa
    23701 gaaggcagca ccttcaacga gagcagctgt aagaatcatg tttgcgcgga tatcgcctga
    23761 tgcctcaggt tgacgtgcaa tagcttctac tgcgctgccg ccaatgcggc caatacctac
    23821 acctgcacct aatgctgcaa gtccggcacc taaagcagca acaccgtaac ctattccaag
    23881 gttgcctgct acttctaata agatggttga taacatatta tttattattt agaatttaga
    23941 aatcaattat ataattaatg cctcctcaac tttggcatcg tgatgttcgg catcgtgatg
    24001 atgttcttca acagcagcac caatataaat agccgaaagt aaggtgaaga cataagcctg
    24061 tagaaatgca actaacaatt caagaaacgt cataaaaacc ataaaggcaa gtgaaatcac
    24121 accaacaccc attcctgcac ctgcactcat ttcaccaaaa ataaaaatca aactaatgaa
    24181 tgataatgct ataatgtgac ctgcagccat gttagcaaac aatcgtatca ttaatacaaa
    24241 tggtcgaaga aacaaaccta atatctcgat gggtgtgagc aaaaacagca cccacatagg
    24301 tacaccgggc atggcaaaaa tatgtagcca ataattttta ttagcactaa atacagttat
    24361 aataagggtc ataacagcca gtgacatggt aatagcaata tttccggtaa gatttgcacc
    24421 accaggaatt aatggaacca gtccaagcaa gttatttatc cagatgaaga aaaatgccgt
    24481 aagcagataa ggcatatatt tctgatattt aggaccaatt gcagatttgg caacatcgtc
    24541 acgaacaaaa acaattaatg gttctataag tgactgtagt ccttttggtg cctgattcgg
    24601 acgtgtttta taagatttag ctacagaagt aaatatccaa atcattaaaa taatgctaac
    24661 gaaaagcgca aaaacatttt tagtaataga gaagtcatat aggttagctg tcaaagattc
    24721 attaattgta gcatgatgtg catctgtttc ttccatttca tcaacagcta caattttttg
    24781 ttctttcagc atatagccat tgtaagtgct atgaccatga tgaaagtgtc ctgatgaaaa
    24841 tacggacaat cctcgctcag gtgaataaac gataatcggc aatggaattt ctgattcgcc
    24901 ccacaagtgc cagccatggg catcagaaat atgatccata atcattcttc cggcatcgaa
    24961 ctcggcagat tctatagatt ctgttgttgc tacacttaaa gaatcatgcg attcctgagc
    25021 catagaaata aaaggaacca gccaaaacag gatagcggat atgaggatgc gtatattcag
    25081 atttttcatt tgtaacatta aggcttgttg ccccttttca aaacggctgc aaaagtagaa
    25141 aagaatccct aaaaagcaat tatagttttg aaaagttatt gaacacctaa tttaacttaa
    25201 tttcgggcaa attttttatc aatgaaactc gatattctgg cttttggagc ccatccggac
    25261 gatgttgaat tagcctgctc cggaaccttg gcaaaacatc ataaaatggg acataaaacc
    25321 ggtgtggcag accttacaga tggtgaattg ggtactcgag gaagtccgga attaagacgc
    25381 aaggaggctt taaatgctgc tcaggaaatg gggcttgatg taagacactt tttgggcttc
    25441 agagatggtt ttttccaaaa tgatgaagcg catagaatta aggttattga ggttttgcgc
    25501 cattttcagc ctgatattgt tattactaat gccccttcag accgtcaccc ggatcatggg
    25561 cgcggttgta atttggttag tgattcctgt ttcttgtcag gtcttaggaa aatcgaaact
    25621 tcttttaaaa accataaaca gcagccttgg cggccaaagt tggtgctcca ttacattcag
    25681 gatcgctatt ttaaaccgga tattgttgta gatatctcaa agttttttga ggtgcgaatg
    25741 aatgctatat tagcttataa atcacaattt catgatcctg aatcggcaga accaaaaact
    25801 tatatttcca gtccggagtt tattgactct ttgaaatcac gtcctgcaga atttggaaga
    25861 ttaataggtg ttgattatgc agaaggtttt ttatttagca gaattaccgg tattaatgat
    25921 ttaaaaagtc tgatttaaac tttatgtaaa caaaaaaggc ggccgaatgg ccgccttttt
    25981 taatgatttt actaagtgat tatttcacta cgaaaagttt agtaactctt gcaccttcag
    26041 ttgtgatggt
//
LOCUS       DAOQNP010000039        25850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_634_length_25850_cov_8.070634, whole genome shotgun sequence.
ACCESSION   DAOQNP010000039 DAOQNP010000000
VERSION     DAOQNP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25850
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_634_length_25850_cov_8.070634"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            613..1845
                     /locus_tag="PKY09_06895"
     CDS             613..1845
                     /locus_tag="PKY09_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017260284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB12621.1"
                     /translation="MPIFENILLALHAVKSQLLRTILTVLIIAIGIMALVGILTAIEG
                     LKASINENFTSMGANTFTIRNSGLGIRLGNSGKKAKRFKAITLQQAELFKTGNNYPSI
                     VSISTMCNGVSVCRVGSEKTNPNILVMAVDENYLQTGGYSISTGRNMTQTEAMRGDNV
                     VMIGSEIKSKLFPDADAVDKEVYIDNRKYRIIGVLAEKGNSMGFGGDKVCLISLINGK
                     QNYGSDNRSWTVSVKVNHVSGLEAAVDEATGLFRTIRKDRVGEESSFEIIKSDSIAAI
                     VIDQLKNVSIAATLIGLITLLGAAIALMNIMLVSVTERTREIGIRKSLGATRLVILKQ
                     FLIEAIVICLLGGVAGVILGVLVGNLMGMAMNAVFVVPWNWIIAGLLLCGFIGIASGF
                     YPAMKASRLDPIEALRYE"
     gene            2058..2426
                     /gene="rpsF"
                     /locus_tag="PKY09_06900"
     CDS             2058..2426
                     /gene="rpsF"
                     /locus_tag="PKY09_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNB12622.1"
                     /translation="MSNQYETVFILTPVLSNEQMNEAVDKFRKILTDNGAMIVHENNW
                     GLRKLAYPIQKKNTGFYYLIEFKSSGELINKLEVEYKRDERVMRFLTVALDKHAIAYN
                     EKKRKNAELKKKEQETATAN"
     gene            2435..2707
                     /gene="rpsR"
                     /locus_tag="PKY09_06905"
     CDS             2435..2707
                     /gene="rpsR"
                     /locus_tag="PKY09_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNB12623.1"
                     /translation="MMSSKQGEIRYLNPVAVDTTKKKYCRFKKVGIKYIDYKDATFLI
                     KFVNEQGKLLPRRITGTSLKYQRKVGQAVKRARHLALMPYVGDLLK"
     gene            2720..3169
                     /gene="rplI"
                     /locus_tag="PKY09_06910"
     CDS             2720..3169
                     /gene="rplI"
                     /locus_tag="PKY09_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNB12624.1"
                     /translation="MEIILKQDVKNLGYADEIVKVKNGYGLNYLIPKGMAVIASEANK
                     KVHTETMKQRAHKMAKIKGDAEKLADAISSVTLTIPVKVGENGKLYGSVTSQNIADSL
                     KKLGYSIDKKQIEMPEEHIRKTGTYSAAVALHRDIKAKINFDVVENA"
     gene            3255..3815
                     /gene="efp"
                     /locus_tag="PKY09_06915"
     CDS             3255..3815
                     /gene="efp"
                     /locus_tag="PKY09_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNB12625.1"
                     /translation="MADTGDIGIGSVIRFNGDLYQVVEYQHRTPGNLRAFYQAKLRNL
                     KNGKLAENRFRSGEQVEVARVEYKTMQYLYKDGENLVVMDNETYEQHYIPEVMIGDSA
                     SYLKEEMMLKIAFEGDNPILAEPPTFVELVITYSEPGVKGDTATNTLKAATLETGAVV
                     QVPLFVNEGEKIKIDTRTGTYVERVK"
     gene            4024..4458
                     /locus_tag="PKY09_06920"
     CDS             4024..4458
                     /locus_tag="PKY09_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB12626.1"
                     /translation="MTIDIPPGIILVEDDDIDRMVVTRAFAKNNIKNPLHIARDGEEA
                     LDMLKGLNGKEKLDPLPKIMLLDINMPRMNGLELLREIRHDEELQTISVFMLTTSKDD
                     QDRLEAYKNNVAGYIIKPVSFDKLVEAIAVLNTYWNLSVLPA"
     gene            4472..6823
                     /locus_tag="PKY09_06925"
     CDS             4472..6823
                     /locus_tag="PKY09_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB12627.1"
                     /translation="MSYKKINLLVVDDDEVDRIAIKRAIKSSGFNADLHFGDTYDEGM
                     EKALSMEFDVLIVDYYLTVMNAGDFISEYFQRGGTAPIIVVSSQGEEKIVVELMKAGA
                     CDYIPKAVLTPETIARSLKGALKSKASKNNHSNIETALVQTERTLNAVVSKSPLILFS
                     INESGIFTLFKGMAMDTIRLSEEDVIGKSVFEIWNRLPVRLKDVKKALLGEEVKQNME
                     FEGRFFEVHYMPSVNSEGVLTGIMGIATDITGHKEQEDELRQQIILSSEAQKMKEQFL
                     ANMSHEIRTPIHGIIGITKIVLQSQLDKDQSHYLNAIRKSADNLLVIINDILDFSKIE
                     AEKMTFESVCFNLFDLINTINELFKARVTDKSVKIIADFDQQLPKLLKGDPVRLSQVI
                     NNLMGNAVKFTHKGSVTLKTELININDSKASIRFKVIDSGIGIAEDKLATIFDSFSQA
                     GTDITRKYGGTGLGLSIAKKIVELQDGSIRVESKLNHGTTFIFEMPFDLPAPGEVETE
                     AKKVAEVHAGFNGKIKILVVEDNDINRLIINKHIKDWGFDHDEACNGLEAIEKLSAND
                     YDVILMDIEMPELNGYAATERIRTEMKGHKSQIAILAMTAHVSASEKEKCIAAGMNDY
                     VSKPFDPTEVKNKIIELSGKANESVTEQPIEETANSQSSSQATTKLTSLSFLNEMSGG
                     NADFIKEFLSLFLTNIPVSIDELEDGLKHKDFEKIRQSAHKMKPSLNYVGLKDTYETV
                     ATLEKYAREKSNLEDYPAMIKKISDECSVACIELESELKNVEV"
     gene            6835..8004
                     /locus_tag="PKY09_06930"
     CDS             6835..8004
                     /locus_tag="PKY09_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB12628.1"
                     /translation="MAKIFIVEDDAMVATLIRQSLSKNPDFEIQHFESAEECLNNLHL
                     NPDIITIDYHLPGMNGVGLMRRIKDYNASIMCILVSGQDSLEVVVDSYKSGAQDYIIK
                     NDNLFVNLENSVKNLSMNVVLKKENEMLKDQIIDRHKYSNIMGSSNAVLRILRLIQKV
                     EKSNMMVLVTGQSGTGKELVARALHYNSPRARKNFVTVNMSAIPEELIESELFGHEKG
                     AFTDARERRIGKFEEANEGTIFLDEIGEMPLSLQAKLLRVLQEKEIQRIGSNKTIKLD
                     FRMVAATNRNLAVEVKEGRFREDLFYRIQGFLIHLPPLKERGDDIILLAKHFLDLYAQ
                     ANKTPVAQLSNEAAKFMLDYEWPGNIRELKAVMERAAILADNATISVDDLMFVNL"
     gene            8096..8389
                     /locus_tag="PKY09_06935"
     CDS             8096..8389
                     /locus_tag="PKY09_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12629.1"
                     /translation="MRKKRKKHLPNRLRQSTKLRLSYRLIAVTATMTMAVVVGGIFYF
                     NISNSKKSKASTTTSDAEQRPVDFNIPDLKQNFREETINGIRVRKMRDEHVTN"
     gene            8376..11459
                     /locus_tag="PKY09_06940"
     CDS             8376..11459
                     /locus_tag="PKY09_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12630.1"
                     /translation="MSRIKIQFSVFLLICFFALSNSVFAVTKTTTASGSWSNPAIWSP
                     AGVPASSDDVVISSAHSITISQPSFCKSLIISGNAVLSLSPSKLLQINGSVNIAGTLD
                     MHGGDIKQLLVNAALQIGASGKLIWNPGDNTLAGASLLLNSVEFFNETSTLEIKKWYS
                     YATVPLAAVVTGNFGNVTISTQVGSTLFEWNQNNLFESHQIKGALTISNGWVVLDKSG
                     SITDTKIGKIILPNVNSIIEFHSGSHPSSFKVTTDSIANIGGEITGILNGEGNITLNV
                     NGGFLNMGNVELIYNKGTLNTANGNAKLNVGGKYMQTHGDIRGVFNLSSTAAGNAEFK
                     FNSLEITGGLFFAYYACNATGALNSVTVTSDMILNFSNASDKFRVNGLTTLAGSYSKS
                     RSLLKVDGNLILSGNAAAEFTSSGSVGAEENRIKGDVNISGLNSNFNMGSHAVVLSVN
                     GDVNINGGNTCFSRTPGILQVSLNKNFSLQSGNFILKGHEGKSDLTLNGAYSQTGGVF
                     YLHSNTLTASDDSIKVYFKNSFRQWNGIINFDDNPEGGKSVIYIQGNEFIAKGSSLIT
                     RAGAGTGSTFGAILYNTNGDLNYQRSTASVIDQIKQYIVANCRLKVTDGNLLISSHNQ
                     PAVDFLKVESGAALNLLSAQIKSNLKYQNSGLTVDAGGTIKTTNAKGFFNTLGTAAVC
                     SNGNMNYFLDKNSIVDYAGNNQLITGSGSGVINKEQFKYGILRVSGSGILASNIEIRN
                     RIELGNSTIKLNQKSLTIESENTNALSSSGGSLICVSESDKLIWKNMKGGSMYSIPFS
                     SDGKVVSKVLVQPIVTGDVSMYNYSANPASENLPDGIQPLIVEGKDVTDESFAKRIVG
                     FYSQVKANITFIFNKNEIPDFNKGDTLVLIKNMGQWEVTPFSTIGDDQNPISIHATNI
                     NANAVFAFCANAGLKIHDNTNGNNSTQGTFSVDQVSPVPFNVFLKVNINAPTVGVVYM
                     TIVGSDGRELRKQMFEVSAGKNILTLSDLSNLPTGVYFLQVTGFKQTITRKIMRQ"
     gene            11557..12696
                     /locus_tag="PKY09_06945"
     CDS             11557..12696
                     /locus_tag="PKY09_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12631.1"
                     /translation="MARKLKLRQRQKMSLMHKAIFVTAGTSIMGLAVYLTVVMNTSNV
                     TDSRAGALTNMIQGYDVNNGEVICAFDWNGDDPTKASKGPDAIKVSPFAVVMSEGVEG
                     TNGLSAGKGKDGIDLTIPTDNYFNTDGIDISFDYKRLDDNCDFISRGRYFNFGMKNGK
                     LVIAYRVNLEKGRSYSVNETTNYELVKDNDFRNYRFVYNPNTGKGEIMVDDVVVWNYQ
                     GPKERSMFWKNSDNIVIAKNMNGGGTDKTILDNFIIRSTTHVQQLPITLLSFQAEARD
                     HHVMVSWYTSRETDIDSFVVERSVDAIEYQKIGAVKANGNSEVLRAYAFVDKTPLENK
                     IAYYRLMPSNKPLKSIAVPIIGYRYRMNHPETAFNPADYPADTVK"
     gene            complement(12747..13829)
                     /locus_tag="PKY09_06950"
     CDS             complement(12747..13829)
                     /locus_tag="PKY09_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNB12632.1"
                     /translation="MKVKILDLYIIKKFLGTFLFAIGLIICIAVVFDISEKVDDFIER
                     NAPLKAIAFDYYLNFIPYFANLFSPLFVFISVIFFTSKMAGQTEIIAILNSGISFNRF
                     LRPYIISATVIAIASFYLNNWVIPHANLVRLQFENIYIKNPYVYRGRNIHRQIKPGEF
                     VYFESYDNKANTGFMFSIEKFEGGKLVTKLMADRITWDSLAQKWSIENYTIRNINGMT
                     EELKKGVRLDTTLSIHPSEFNRRMNFIEAMDFSELNNYIKEEKQRGSEVIPFYEVEKY
                     RRTSYPFATFILTLIGVAISSKKVRGGIGLQLGAGILLSFTYIMFMQVSTTFATNGNV
                     PAVVAVWIPNLVYAFIAIYLLRKAPK"
     gene            13967..15343
                     /locus_tag="PKY09_06955"
     CDS             13967..15343
                     /locus_tag="PKY09_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNB12633.1"
                     /translation="MRYHFIAIAGSAMHNLALALHHLGHDVSGSDDEIVEPAKTRLAK
                     VGILPEKTGWFPEKINADIDAVILGMHARADNPELAEAIRKKIKVFSYPEFLYEHSKN
                     KTRVVIGGSHGKTTITSMILHVLKTSGINFDFMVGAQLEGFDTMVKLSNDAKVIILEG
                     DEYLSSPIDRRPKFHLYHAHIGLISGIAWDHINVFPSLEIYEEQFRIFAREIPDDGKL
                     FYCLNDATVKTVCEAEDVKALKTGYTIPKHSIKNGKTVIYDDRNNEVPLQIFGEHNLM
                     NLEGARMVCNSIGISNDEFYTAIKSFKGAARRLELLSESNQFAVFRDFAHSPSKLKAT
                     LEAVRKQFPERKLIACMELHTFSSLNENFLEEYNGTMDEAEVAVVYFNPKTIEHKKLP
                     PITSEKIFKSFGRSDIKIMNDSIQLMNYLNSEDKQQVVYLMMSSGTFDGLDLKSFAER
                     LSTSHQGV"
     gene            complement(15344..16168)
                     /locus_tag="PKY09_06960"
     CDS             complement(15344..16168)
                     /locus_tag="PKY09_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNB12634.1"
                     /translation="MPLPKSEWKYDLISTIFPSKFLKREVRIDYYLPSQYAKNTGVLF
                     MLDGQDAELLQLKDTLQHLTQLKKIKPIIVIAIHANKNRLQEYGVAGKADYLKRGNKA
                     KQFSKFILDELIAQCSSFTGIEFTPSKTAIAGFSLSGLTAFDLAWNHNAVFETAGVFS
                     GSFWWRKKSYEDGYTDDDRIMHQVIRKSKSAPSIKCWIQTGTKDETADRNKNGVIDSI
                     DDARDLIVEMKQKGVPSKNIQYVEIPEGQHNQVTWSAALPLFLCWAFGNKRKVDIY"
     gene            complement(16187..17728)
                     /locus_tag="PKY09_06965"
     CDS             complement(16187..17728)
                     /locus_tag="PKY09_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNB12635.1"
                     /translation="MSLTEQFKEVVNKEYQFKGDSIVLGAPVLKGETLTGCFIKAPLK
                     TFNRHGLIAGATGTGKTKTMQGIAESLSAKGVSCLLMDVKGDLSGLASPGTTNPKIDE
                     RYQKLGLTWKPLQFPVEFLTLSDEKGVRLRATVSEFGPVLFSKILDLNDTQQGVVSLI
                     FKYCDDKQLPLLDLKDFKKVLQYLTNEGKDEIKADYGAISSASASTIIRKIIEIEQQG
                     AEKFFGEKSFEVEDLLGIDENGNGKLNIIRLTDIQDKPKLFSTFMLSLLAEVYSTFPE
                     VGDPEAPRLVIFIDEAHLIFNNASKVLLDQIETVIKLIRSKGIGVFFVTQSPEDIPAA
                     VLAQLGLKVQHAFRVFTANDRKALKLIAENYPLSDYYKVDELLTTLGIGEAAITVLNE
                     KGVPTPIAHTLLCTPASRMDILTPDELAKSISKSRLTTKYNETLDRESAFEVLSKKLE
                     TQVAENTKTTKGAEAKEEPSQLDQVLNSSTGKQVMRTVTSVLTRSLLGALGLGATRRK
                     KSGWF"
     gene            17838..20216
                     /locus_tag="PKY09_06970"
     CDS             17838..20216
                     /locus_tag="PKY09_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNB12636.1"
                     /translation="MPEGSSVFQRIYKQLFLILKSLITFSYLKIIKNKALRGVAILFM
                     LMVYYIVLLHINFLWLFGYMPKMSEVRNPQVAIATEIYTEDSVLIGKYYHENRTPVRF
                     DSISTYAVQALIATEDVRFYKHFGLDLYALISGAYSTAKGDERGASTITQQLAKNMYS
                     TRKKVNQGLLQHIPLVGTLVSKSKEWVTSVKLEMFYSKDEILEMYFNTVDFGNNWYGV
                     KVAAQNYFSKQPSELNLQESAMLIGLLKATSSYNPLNNKKRSLTRRNIVLSQMQKYGY
                     ISQATYDSISPLPLNLKRPTNNPATKDSYLRQLVAKIIKSYCDENSININEDGLKIYT
                     TINSHLQNYAELAVKDHMKKVQKQFDEHWGKQNPWRDDNGREIMNFTNTALAFTQSYD
                     RLKKQFPSSPDSITYYLNKKKVMKVFSYNGSIDTVMSTIDSLKYYARLLNAGTMSLNP
                     LTGEIKAYVGGVDFDFFKFDHVVQSKRQPGSTFKPFAYLAAIEDTFSPCSRLDDVPVS
                     IEYDGGQIWQPYNSTGAFTYSSKTLRRALAQSVNSITAQIAELVGWDKIVECAHRAGV
                     KSRLDTVPSICLGTSDVSVYEMTCAYAPFVNGGKSVEPILVKRICANDGTLLKEFVPE
                     FKQVITEENAWLMLYMLQGTIQESGGTSQALWGYSIFKNGNDVAGKTGTTSNYSDAWY
                     MGVTHDLVTGVWVGAPYRSVHFRNSSGQGSRLALPIFGKMLEKAYQDPKSGVVLGKFK
                     KPKKISKTIYCDNDDIELEAADSLLNDSLINTELMILENDSVKSDSLQGGRD"
     gene            20244..20999
                     /gene="tpiA"
                     /locus_tag="PKY09_06975"
     CDS             20244..20999
                     /gene="tpiA"
                     /locus_tag="PKY09_06975"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNB12637.1"
                     /translation="MRRKIVAGNWKMNGTLDDAIKLTSEIAGMYKDEINSDVQVVLSP
                     PFPFLNSVSKLTNNTAIKVAAQNCAAQASGAFTGEVSASMITSCGAGYVILGHSERRT
                     YFSDTDEVLLLKVRQALLNGLQVIFCIGETKAERESNSHFDCVEKQILNVLSQLTPDE
                     FAKCIVAYEPVWAIGTGLTASPAQVQEMHHFIREVLKKQFGEQASETSILYGGSCNEN
                     NAKELFALPDVDGGLIGGASLKSRSFISIIKSF"
     gene            21004..22170
                     /locus_tag="PKY09_06980"
     CDS             21004..22170
                     /locus_tag="PKY09_06980"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNB12638.1"
                     /translation="MSNSFKSVLLFAFLVCSLFSEAQSLSQPAFVEYSFSTGRVVKNY
                     PVFPERKLAGVNSFIIGSRLNGIKAWHPYYNFPNASFELAYGSLGSNKVLGNFSALLY
                     RLEFGYKLNDQFYTEITPAFGIAYFNKPFHADKNPENTLIGSAFTFCAAARLNLRYYI
                     NPFWSVSAGAGVYHCSNSHYQLPNLGINLPFASAGLRYHIRPLNLLLGSRSIEKDSGY
                     HFQVRFAVGYNEQGASTFPVNGPRYPIYLGAFYVTRYLTPVNKLHLGVEGWYNSGVYD
                     FITSQRYYNDKEHLRSTAWQAVVGNEFLFGHFSMLINGGIYFYNPVYHEKLRRENETN
                     FKKQLKGWITARLGFQYYLKDATIYHKSNFYAGVYIKTNLGQADFLETGIGYAF"
     gene            22257..23201
                     /locus_tag="PKY09_06985"
     CDS             22257..23201
                     /locus_tag="PKY09_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNB12639.1"
                     /translation="MKNRITELFGIEYPIVQAGMIWCSGWRLASAVSNAGGLGLIGAG
                     SMYPDVLKEHIEKCKVATNKPFGVNVPLLYPDIEKLMAIIVAGGVKIVFTSAGNPATW
                     TNYLKQAGITVVHVVSNVKFALKSQAAGVDAVVAEGFEAGGHNGREETTTMCLIPEVK
                     DALQIPVIAAGGIGSGRAMMAAFALGAEGVQVGSRFVASVESSAHTNFKDKVINTSEG
                     QTVLTLKQLTPVRLIKNKFSEQVEQAQQRCATREELTQLLGRARAKKGMFEGDLNEGE
                     LEIGQVSSQIKNIIPARDIVKNIWNEFLKEKNRIANLF"
     gene            23205..24452
                     /locus_tag="PKY09_06990"
     CDS             23205..24452
                     /locus_tag="PKY09_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB12640.1"
                     /translation="MMKFEKIVLPNGLPVIVNYDPSTPLVAMNILYKVGARNEDAGRT
                     GFAHLFEHLMFGGSLNIPSYDEPLQRAGGENNAFTNNDFTNYYLTIPSQNVETAFWLE
                     SDRMLSLAFSEKSLDVQRNVVIEEFKQRYLNQPYGDVWLNLRPLAYKVHPYQWATIGK
                     DIKHIEDATLDDVKAFFHKWYCPNNAILTLSGNIKPEYAFKLSEKWFGEINAHDLSKE
                     SLPKEPKQNEYRNIILERDIPANSIYKAFHTCSRFDDEFYATELLTDILCGSKSSLLY
                     HTLVKEQQLFNSINAYFTGDLDNGLMVVEGKVNDGVEVKNADHAVDVLLQQVADDGVQ
                     EKEMKIVKQKTESALIFSEVNIGNRALNLAYFEMIGGAEKINKQLQQYEAVNSNDVQQ
                     VAKTILKKENESSIYYCKKGVTL"
     gene            24449..25717
                     /locus_tag="PKY09_06995"
     CDS             24449..25717
                     /locus_tag="PKY09_06995"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB12641.1"
                     /translation="MNRVAEPALTLISKIDITLPEKIVLDNGIQVFVLNAGEQEVTKL
                     DIVFRAGDWYGEFSTLAANVNELLDSGTAKRTAYEISQSFDQYGAYLNTTCNFDTASV
                     KLFTLSRFIPETFSLLLEIISEAIFPDEEVKILRDQSIQQLKINSQKVNYISRNNFNK
                     MLFPGDHPYGRSSSVADYQKLNSADLLLWHNQYYQPQNCLIFLSGRFNEDAIKSLNQI
                     FGKWKDTSPGKIIEKNFSVENYKPEKKYIEKNDALQTAIRIGCHAPNKLHPDYIPLTV
                     LSTVLGGYFGSRLMSNLREERGYTYGVGCTLVSWLHDGYFLISTQVGKQHRERAIEQI
                     YLEIKRLLEEKISDNELNRVKNYMTGVYQRSLDGPMLVSDRIRSTVLYNQDFNVLNTY
                     LQIMNSTNSDQLIELANRYFSNEMTEVSVG"
BASE COUNT         8493 a         4160 c         5169 g         8028 t
ORIGIN      
        1 ttacagtata taggttttgg cggcaagcac caacctctgc cggaggtgtt agagaaacat
       61 ttaccgtttg tgattgcgct cttgtaaaga atgtaaacat gcagatcaaa atgaccaagg
      121 tatttgtttg gatttggttt ttcatattat ttggtttttg ggtgtgacta aattacattg
      181 attttttgga ttttatagcg attgcttaaa aattaaaacg caggctgatt cccttgctcg
      241 tcatagtggg agtcgaagcc cagcacacga agcaaaaagg aacgcccacg ttttaagtag
      301 gcgtttctac ttttactttc ctgtgtgctt gaaatcttcg acttttctat gacaggaaat
      361 tttaaagcga aacgcttttt attttttact tatttgtttt ctctatctta tttattttta
      421 atctatttct attttgtaaa agtgtacaat attattgaaa ctaacaaata tttatttaat
      481 agttcacatt gtctctggaa aattcttcac cttgtatgct tgaattttca aaacttaagc
      541 ctttttgaaa aaaattaact ttcattatga gttaaaacct ttcggggcaa agattaattg
      601 taatttagcg ccatgcccat ttttgaaaat atacttttgg ctctacatgc tgtaaagtca
      661 cagttgctca gaacaatact gacagtgtta atcattgcca taggcataat ggctttggta
      721 ggaatactta ctgccatcga aggccttaag gcatccatca atgaaaactt caccagcatg
      781 ggagcaaaca ccttcactat cagaaattca ggcttgggaa taaggttagg taattccggc
      841 aagaaggcta aacgttttaa agcaattacc ttacagcagg cagagttgtt taagaccggt
      901 aataactatc cttctattgt aagtatttca accatgtgta atggagtgtc agtttgtcgt
      961 gtaggttctg aaaaaaccaa tcccaatata ttggtcatgg ctgttgatga aaactatctg
     1021 caaaccggag gttattcaat ttccacaggt cgcaatatga cacaaacaga ggcaatgcgt
     1081 ggcgataatg tggtgatgat tggctctgaa ataaagagta aactttttcc tgatgctgat
     1141 gctgttgaca aagaagttta tatagacaac agaaaatata gaattattgg cgtacttgcc
     1201 gagaaaggta acagcatggg ttttggtggt gataaagtat gtttgatttc attaatcaac
     1261 ggtaagcaaa actatggcag tgataatcgt tcatggacag ttagtgttaa ggtgaatcat
     1321 gtgtcaggcc ttgaagcagc agttgatgaa gcaacaggac tattcagaac aataagaaaa
     1381 gatcgtgttg gcgaagaaag ctcatttgaa ataataaaaa gcgacagcat tgcagcaatt
     1441 gtaattgatc agttgaagaa tgtaagtatt gcagcaacac tgattggact tatcacattg
     1501 cttggtgcag caattgcatt gatgaatatt atgcttgtct ctgtcacaga gcgaacccgc
     1561 gaaattggaa tcagaaaatc gcttggtgca acaaggcttg taatcttaaa gcagtttttg
     1621 attgaagcaa ttgttatttg cctcttgggt ggcgtagcag gagttatttt aggagtgctg
     1681 gtcggcaacc tgatgggaat ggctatgaat gctgtttttg tggtgccttg gaactggatt
     1741 attgccggat tgttgctatg tggttttatt ggcattgcct caggttttta tcctgctatg
     1801 aaagcttcgc gcctcgaccc aattgaggct ttacgctacg aataatatat ttgatcattc
     1861 taattcatct ggattatttt attatatagt tgattttcaa atttttaata taatttactt
     1921 aaagtaattt attgtagcga ttcgcttagg atgagtatct taaaaaaaag taaaaattct
     1981 attttaagaa attaataata acactatctt tgccgtccgc ttaaaaaagg ctattaaact
     2041 gaaatatata taaacaaatg tcaaaccagt acgaaaccgt attcattctt acacccgtac
     2101 tcagcaatga gcagatgaat gaagcggttg acaaattcag gaaaatcctc accgacaacg
     2161 gtgccatgat tgttcatgag aacaattggg gattacgcaa gcttgcttat ccgattcaga
     2221 agaagaacac gggcttctat tatctgatcg aatttaagtc aagcggtgaa ctcatcaaca
     2281 aattggaagt cgaatacaaa cgcgatgagc gtgtgatgcg attccttact gttgcgctcg
     2341 ataagcatgc cattgcttac aatgaaaaga agagaaagaa tgcagagttg aagaagaaag
     2401 aacaagaaac agcaactgct aattaaacga aggaatcatg agctcaaaac aaggagaaat
     2461 taggtacctc aacccggtag cggttgatac cacaaagaag aaatactgtc gtttcaagaa
     2521 agttggaatc aagtatattg actataaaga tgccacattc ctgattaagt ttgttaatga
     2581 acaaggcaaa cttttaccac gcagaataac cggtacatca ttaaaatacc agcgtaaagt
     2641 tggtcaggct gttaaacgtg cgcgccatct tgccctgatg ccttatgttg gagatttgtt
     2701 aaaataggag gaggcaacaa tggaaattat attaaaacaa gatgtgaaaa atctcggcta
     2761 tgccgatgaa attgtgaagg taaaaaatgg ctatggcctg aattatctta tccctaaggg
     2821 aatggctgtt attgccagtg aagccaacaa aaaagtgcat acagaaacaa tgaaacaacg
     2881 tgcgcacaag atggcaaaaa tcaaaggtga tgcagagaaa ttagctgatg ctatttcaag
     2941 cgttaccctg acaatacctg taaaggtagg cgaaaacggc aagctttatg gttctgtaac
     3001 ttctcaaaat attgctgact cattgaagaa attgggttat agtattgata agaagcaaat
     3061 cgaaatgcct gaagaacaca ttcgtaaaac aggaacatac agtgcagctg ttgcattgca
     3121 tcgcgacatc aaagcgaaaa ttaattttga tgtagttgag aatgcttaat cagttaaagt
     3181 aaataaatta ataaacaggc agcgctttat cacgttgcct gttttgtttc attaacacat
     3241 aaataaaaaa gaatatggca gatacaggcg atataggaat tggttcagta atcagattca
     3301 acggagattt atatcaggtt gtagagtatc agcatcgtac accgggcaac cttcgtgctt
     3361 tttatcaggc aaaactgcgt aacttaaaaa acggaaaact tgcagaaaat cgcttccgtt
     3421 caggtgaaca agtagaggtg gcacgtgttg aatacaaaac catgcaatat ctctataaag
     3481 atggagagaa cttggtggta atggataacg aaacttatga gcagcattat attcctgagg
     3541 tgatgatagg cgacagtgcc agctatttaa aggaagaaat gatgctgaag attgcttttg
     3601 aaggtgataa tcctatactt gctgagccac caacatttgt tgaattagta attacttaca
     3661 gcgaacccgg tgttaaaggc gatactgcaa caaacacttt aaaagcagca actttagaaa
     3721 caggtgctgt cgttcaggtt ccgctttttg taaatgaagg cgaaaaaatt aaaattgata
     3781 cgcgcaccgg tacctatgta gaacgcgtaa aataaaatct aaattttttt taattaaaac
     3841 ctcgcttcag tcgaggtttt tttattgccc tttttgaact ataaattata gttgaacctg
     3901 ttttctccaa tcttttccat caaaattacc tgaaatctta cattttgctt tttgcaacat
     3961 acaggccatt cagtaaccaa tagtgctgat agcagtataa gttgtattca aaaatgatca
     4021 ggtatgacaa ttgatatacc accgggaata attttagttg aggatgacga cattgacaga
     4081 atggttgtta ctcgcgcatt tgccaagaat aatattaaaa acccattgca cattgctcgt
     4141 gatggcgaag aagccttaga tatgcttaag ggtttgaatg gtaaagaaaa acttgacccc
     4201 ttgcctaaaa ttatgttact cgatattaat atgccgcgta tgaatggtct tgagttgctt
     4261 cgtgaaatca gacatgacga agaacttcaa accattagtg tttttatgct tacaacatcc
     4321 aaagacgatc aggacagatt ggaagcatat aaaaacaacg tagcaggtta tattatcaaa
     4381 cccgtgtctt tcgacaaatt ggttgaagct atagctgtat tgaatactta ttggaacctt
     4441 agtgtgttac ccgcttaaat aattttttat tatgtcttac aaaaagataa acctgttagt
     4501 tgttgatgat gatgaagtag atagaattgc aattaagaga gcaataaagt catcaggatt
     4561 taatgccgat ttgcattttg gcgataccta cgatgaaggt atggagaaag cattaagtat
     4621 ggaatttgat gttttgatag ttgactacta tctgactgtg atgaatgcag gtgatttcat
     4681 ttcagaatat tttcaacgtg gtggcactgc accaatcatt gttgtttcat cacaaggtga
     4741 agaaaaaata gttgttgaac tgatgaaggc cggtgcctgc gattacattc ctaaagctgt
     4801 actaacacct gaaacaatag cacgttcctt aaagggtgca ttaaaatcga aagcatccaa
     4861 aaacaatcat agcaatattg aaactgcact tgtgcaaaca gagagaactc tcaatgcagt
     4921 ggtttcaaag tcgccattaa ttctgttcag catcaatgag tcaggcatat ttaccttgtt
     4981 taaaggtatg gcaatggaca caatccgttt gtcagaagaa gatgtaattg gaaagtctgt
     5041 ttttgaaatc tggaatagac tacctgtaag actgaaggac gttaagaaag cattgcttgg
     5101 agaagaggtt aagcagaaca tggagtttga aggccgattt tttgaagtgc attacatgcc
     5161 atcagttaat agcgaaggtg ttcttaccgg aatcatgggt attgctactg acattacagg
     5221 tcataaggaa caggaagatg aattgcgaca gcaaattatt ttatcgagcg aagctcagaa
     5281 aatgaaagag cagtttctgg caaatatgag ccatgaaatt cgtacgccta ttcacggaat
     5341 tattggtatc acaaaaattg ttctgcaatc acagcttgat aaagaccaat cgcattattt
     5401 aaatgcaata agaaaatcag cagataatct tttagtaatt atcaacgaca ttctcgactt
     5461 ctcgaaaatt gaagctgaga aaatgacatt cgaatcggtc tgtttcaatc tgtttgatct
     5521 tattaatacc atcaatgaat tgtttaaagc tcgtgtgaca gataaaagcg ttaagataat
     5581 agcagatttt gatcagcagt taccaaagtt gttaaagggc gatccagtta gactgagtca
     5641 ggttattaat aaccttatgg gcaatgccgt aaagtttaca cataaaggat ctgtaacact
     5701 taaaacggaa ctcattaata ttaacgactc aaaggctagc ataaggttta aagttattga
     5761 ctccggtatt ggcattgccg aagataaact tgctacaatc tttgattcat tctctcaggc
     5821 tggaacagac attacacgta agtatggcgg aacaggattg ggtttaagta ttgcaaaaaa
     5881 gattgtagaa ctgcaggatg gaagcatacg agttgaaagt aagttgaatc atggcacaac
     5941 attcattttc gaaatgccat ttgacttacc tgcacccgga gaggtggaaa cagaagcgaa
     6001 gaaggttgct gaagtacatg ccggttttaa tggaaagata aaaatcctgg ttgtagaaga
     6061 taatgacatc aatcgtttaa tcataaataa acacattaaa gattggggtt ttgatcatga
     6121 tgaagcttgt aatggtttgg aagcaataga gaaattgtct gccaatgact atgatgttat
     6181 tttgatggat atagagatgc ctgaattaaa tggttatgca gctacagaaa gaatcagaac
     6241 agaaatgaaa ggtcataagt cgcagattgc aattttagcc atgacagcac acgtttctgc
     6301 aagcgaaaaa gaaaaatgta ttgcagccgg aatgaatgat tatgtctcga aaccatttga
     6361 ccccactgaa gtaaaaaata aaattattga acttagtggc aaggctaatg agtctgttac
     6421 agagcagcct attgaagaaa ctgccaatag tcaaagtagt tctcaggcta caacaaaact
     6481 gacaagcctt agttttctga atgaaatgtc gggcggaaat gcagatttta ttaaagagtt
     6541 tctatcattg tttttgacta acataccggt gtccattgat gaactagaag atggtttgaa
     6601 acataaggac tttgaaaaaa tcagacagtc tgcacataaa atgaaacctt ccttgaatta
     6661 tgtaggttta aaggatacgt acgaaacagt agcaacattg gaaaagtacg ccagagagaa
     6721 gtcgaattta gaggattatc cggcaatgat taaaaagatt tctgatgaat gtagcgttgc
     6781 ctgtatagaa cttgagagtg aactaaaaaa tgtagaagta taaataataa aactatggct
     6841 aaaattttta ttgtggaaga tgatgcaatg gtggcaacac tgatacgcca gtcattgtca
     6901 aaaaatcctg atttcgagat tcagcatttt gaatcggcag aggaatgttt aaacaatctg
     6961 catcttaacc ctgacattat aactattgac tatcacttac cgggaatgaa tggtgtaggc
     7021 ttgatgcgaa gaattaaaga ttataatgca tccatcatgt gcattcttgt ttctggacaa
     7081 gacagccttg aagtggtcgt tgattcttat aaaagcggag cacaggacta cataatcaag
     7141 aatgataatt tgttcgtgaa tcttgaaaac tcagtaaaaa atctgagcat gaatgtggtg
     7201 ttgaagaagg agaatgaaat gctaaaagac cagattattg accgtcataa gtacagcaat
     7261 ataatgggta gcagcaatgc tgtgttaaga attttacgtc tgattcagaa agttgaaaaa
     7321 agcaatatga tggtgctggt tacaggtcaa agcggaacag gtaaagaact tgttgcacgc
     7381 gctttgcatt ataattcacc tcgtgcccgt aaaaattttg ttacggttaa tatgagcgcc
     7441 attcctgaag agcttattga aagtgaactt tttggtcatg agaagggagc atttactgat
     7501 gcccgcgaac gcagaattgg aaagtttgaa gaagctaatg aaggaactat ttttcttgat
     7561 gaaataggtg agatgccttt aagcttacaa gctaaattgc ttcgtgtgtt acaggaaaaa
     7621 gaaattcaac gcattggaag taataaaaca attaagctcg atttcagaat ggtggcagcc
     7681 actaacagaa atttggctgt tgaggttaaa gaaggacgat tcagagaaga tttattctac
     7741 aggattcagg gattcctgat tcatttgcca ccattgaaag aaagaggtga tgatattatt
     7801 ctgcttgcaa agcacttcct tgacctctat gctcaggcta ataaaactcc tgtggcacaa
     7861 ctctccaatg aagcagctaa atttatgctt gactatgagt ggcccggaaa tattcgtgaa
     7921 cttaaagctg tgatggaaag agcagctatt cttgcagata atgctactat ttctgtagat
     7981 gatttaatgt ttgttaattt ataaatcagt actgaatgat attattaagc cgcaccgtaa
     8041 tgcggctttt tttatgcaac ctttttagcg attttacata taaggattta cgcagatgag
     8101 gaagaaaaga aagaaacacc tgcccaacag gctaagacaa agtactaagc tacgactgtc
     8161 ttatcgttta attgccgtta ctgcaacaat gacaatggct gttgttgttg gcggaatatt
     8221 ctattttaat atctccaact ccaaaaaatc aaaggcaagt actacaacat cagatgcaga
     8281 gcaaagacct gtagatttta atattcctga tttgaaacaa aatttcagag aagaaactat
     8341 taatggtatc agagtaagaa agatgaggga tgagcatgtc acgaattaaa atacagtttt
     8401 ctgtcttttt attgatttgt ttttttgcat tgagcaattc tgtatttgct gttacaaaaa
     8461 ctacaacagc ttcgggtagt tggagtaatc ctgctatttg gtcacctgcc ggagtgcctg
     8521 cttcttctga tgatgttgtt atctcatctg cacatagtat aacaatatca caaccatcat
     8581 tttgcaaatc acttattatt tcaggaaatg ctgtacttag tttgtcgcca tcaaaattac
     8641 ttcagataaa tggaagtgtc aacatagcag gtacacttga tatgcatggt ggtgacatta
     8701 agcaacttct tgttaacgca gcattacaga ttggtgcttc aggcaaattg atttggaatc
     8761 ctggagataa cacgttggcc ggtgcatcac ttttactgaa ttctgttgag tttttcaatg
     8821 aaacttcaac tttagaaatc aaaaaatggt attcctatgc aactgtacca ttggcagctg
     8881 tagttacagg taattttggg aatgtcacta tcagcactca ggtggggagc actttgtttg
     8941 aatggaatca gaataatcta ttcgaaagtc atcagattaa aggtgcatta accataagca
     9001 atggatgggt tgtgttggat aagtcaggta gtattacgga tactaaaatt ggtaaaatta
     9061 tcttgcctaa tgtcaattcc ataattgagt ttcatagtgg tagtcatcca tcatcattca
     9121 aggttactac tgacagtatt gcaaacattg gtggtgaaat aacaggtatt cttaatgggg
     9181 aaggcaatat tacattgaat gtaaacggtg gctttctgaa tatgggaaat gtagagttaa
     9241 tctacaataa aggaacttta aatacggcta atggaaatgc aaagctcaat gttggaggca
     9301 agtacatgca aacacatgga gacattagag gagtctttaa tctttcttca acagctgccg
     9361 gaaatgcaga gttcaaattt aattcacttg aaataacagg tgggttgttc tttgcttatt
     9421 atgcttgtaa tgcaacaggt gcattaaatt ctgttactgt tacaagtgat atgatactta
     9481 acttttcaaa tgcatcagat aaattcagag ttaatggact tacaacatta gcaggctcgt
     9541 atagcaagtc gcgttcatta ctaaaggttg atggtaacct gattctttcc ggtaatgcag
     9601 cagcagaatt tacttcaagt ggaagtgtag gtgcagaaga aaacagaatc aaaggtgatg
     9661 tgaatatatc aggactaaac agtaacttca acatgggaag ccatgctgtt gttttatctg
     9721 tgaatggaga cgttaacata aatggtggaa acacctgttt ttctagaacc ccgggaattt
     9781 tacaagttag cttaaataag aatttctcat tgcaatcagg caattttatt ttaaaaggtc
     9841 atgaaggaaa gtctgacctt acgttgaatg gtgcatattc acagaccggt ggtgttttct
     9901 atttacattc gaatacatta actgcatcag atgattctat aaaagtttac tttaaaaatt
     9961 cattcagaca gtggaatggt atcatcaact ttgatgacaa tcctgaaggt ggtaaatctg
    10021 taatttatat tcagggaaat gaatttattg ccaaaggcag ctctttgatt acaagagcag
    10081 gtgccggaac cggaagtact tttggagcta ttttatataa tacaaatggt gatctgaatt
    10141 atcaacggag tacagcatct gttattgatc agattaaaca atatattgta gcaaattgca
    10201 ggcttaaagt aacggatgga aatttattaa tcagcagtca caaccagcct gctgttgact
    10261 ttttaaaagt agaatccgga gcagcattga atttactaag tgcacaaata aagtctaatc
    10321 taaagtatca gaattcaggt ctcaccgtag atgcaggagg aacaattaaa actacgaatg
    10381 ctaaaggttt ttttaacacg ttgggtactg ccgcagtttg tagtaatgga aatatgaatt
    10441 actttcttga taaaaacagt atcgttgatt atgcaggaaa taatcagttg ataacaggat
    10501 cgggttctgg cgttatcaat aaagagcagt ttaagtatgg aattttaaga gtgagcggaa
    10561 gtggcattct ggcatcaaac attgagattc gtaaccgtat cgaattaggc aattcaacaa
    10621 ttaagctgaa tcaaaaatct ttgaccatag aaagtgaaaa caccaatgca ttgtcatcat
    10681 cgggtggctc attgatttgt gtttcagaat cagataagct catctggaaa aatatgaaag
    10741 gtggaagtat gtattcaatt cccttctcaa gtgatggtaa ggttgtttca aaagttttag
    10801 tacaaccgat tgttaccggt gatgtttcca tgtataacta ttctgcaaat ccggcatccg
    10861 aaaacttgcc tgatggaata caaccgctta ttgttgaagg aaaagatgtt actgatgaat
    10921 catttgcaaa acgtattgtt ggtttctatt cacaagttaa ggcaaatatt acttttatat
    10981 tcaataagaa tgaaatacct gacttcaaca aaggtgatac cttagttctt attaaaaata
    11041 tggggcagtg ggaggtaaca ccgttcagta caataggtga tgatcaaaat ccaatttcta
    11101 ttcatgcaac aaatattaat gctaacgcag tttttgcttt ctgcgcaaat gcaggactta
    11161 aaatacacga taataccaac ggaaacaata gtacacaggg tacttttagt gtagatcagg
    11221 tttcacctgt accttttaac gtttttctta aagtgaacat taatgcacca actgttggtg
    11281 tggtttacat gacaattgta ggctctgatg gccgtgaact tagaaaacag atgtttgaag
    11341 tttctgctgg aaaaaacata ctaacgctta gtgacctgag taatttaccc acaggagttt
    11401 attttttaca ggtaaccggt ttcaagcaaa ccattacaag gaaaataatg cggcagtaat
    11461 gaaatgtaag aaatctgaaa tttcgattat agtttttaca acctttttta gtttctaaga
    11521 gtataatcgt aaggttcatt ctataaatta attattatgg caagaaaatt aaaactccgt
    11581 caacgacaaa aaatgtccct aatgcataag gcaatttttg ttactgccgg aacatcaata
    11641 atgggattgg cagtatatct tactgtcgtt atgaatactt ccaatgtgac cgactcacgt
    11701 gctggagcac ttaccaacat gattcagggt tatgatgtaa ataatggaga agtaatatgt
    11761 gcgtttgact ggaatggtga tgatcccaca aaggcaagta aaggacctga tgcaataaaa
    11821 gtaagcccat ttgctgtggt aatgtcagaa ggtgttgaag gtacaaacgg attgagtgcc
    11881 ggtaaaggta aagacggaat tgatttgact attccaactg ataattattt taacactgat
    11941 ggaattgata ttagttttga ttacaaaagg ttagatgata attgcgactt catcagtcgt
    12001 ggacgttatt ttaactttgg aatgaaaaac ggaaaattag tcattgccta ccgtgtaaat
    12061 ctcgaaaagg gtagaagtta ttcagtgaat gaaacaacta actatgagtt ggtaaaagat
    12121 aatgatttca gaaactaccg gtttgtttat aatcctaata caggaaaggg tgaaattatg
    12181 gttgatgatg ttgtggtttg gaattatcag ggacctaagg aaagatcaat gttctggaaa
    12241 aattctgaca atattgtaat cgcaaagaat atgaatggtg gcggtacaga taaaactatt
    12301 cttgataatt ttattattcg tagcaccaca catgtgcaac aactcccaat cactttgctt
    12361 tcttttcaag cagaggctcg tgatcatcat gtcatggtaa gttggtacac atcacgcgaa
    12421 acggatatag actcctttgt tgttgaacgc tctgttgatg ctattgaata tcaaaaaata
    12481 ggagctgtta aagctaatgg gaattcagaa gtgttgcgtg cttatgcatt tgttgataaa
    12541 acgcctttgg aaaacaaaat tgcctattac aggttgatgc cttcaaacaa accattaaaa
    12601 tcaatagcgg tgccgattat aggttatcgt tatagaatga accacccgga aacagcattt
    12661 aatccggctg attatccagc tgacacggtg aaataaaatt ttatccgtta ggtagaagag
    12721 gctgtcattt aaatgacagc ctcttattat ttgggtgctt ttcgtaataa atagatagca
    12781 ataaatgcat acactaaatt aggtatccat acagcaacaa cggcaggcac atttccgttt
    12841 gttgcaaatg ttgtgctaac ttgcataaac ataatgtatg taaagcttaa taaaattcct
    12901 gcccctaatt gtaatcctat accgccacga acttttttac tcgatatggc tacaccaatt
    12961 aatgtaagta tgaatgttgc aaaaggatag gatgtacgtc tgtatttttc aacttcgtaa
    13021 aaaggaatga cctctgatcc acgttgtttt tcttccttaa tatagttgtt taactccgaa
    13081 aaatccattg cttcaataaa gttcattctt ctgttaaatt cagatggatg tattgataat
    13141 gtagtatcta atcttactcc tttttttaat tcttctgtca tgccattaat attcctgatg
    13201 gtataattct caatactcca cttttgtgct agtgaatccc atgtaattct atcagccatg
    13261 agtttggtaa ccaactttcc tccttcaaat ttttcaatgc tgaacataaa acctgtgtta
    13321 gctttattgt cgtagctttc aaagtaaaca aattcacccg gtttaatttg tctatgaata
    13381 tttctaccgc gatagacata agggttttta atatagatat tttcaaattg aagccgtacc
    13441 aggtttgcat gtggaattac ccagttgttt aaataaaaag atgcaatagc aatgactgtt
    13501 gcagaaataa tataaggtcg gagaaagcgg ttgaaactga taccactgtt taaaatagca
    13561 ataatttctg tttgtccggc cattttggaa gtgaaaaaaa tgactgaaat aaaaacaaat
    13621 aatggactga ataaattggc aaaataaggt ataaagttga ggtaataatc aaatgcaata
    13681 gcctttaatg gggcatttct ctcaataaaa tcatctactt tctcagaaat gtcaaacaca
    13741 acggcaatac atataattag tcctatggca aacagaaatg tgcctaaaaa cttcttgatg
    13801 atgtagagat ctaagatttt tactttcaat gcaaactatt tggcgcaaaa ctaaattaaa
    13861 tcacttatct tttgtaacaa gaatagagat gttaatcgtt aataaaaagt ttgaaagatt
    13921 taaatattca ttttatgacc atgtatttta gcgataaaat tcagaaatga gatatcattt
    13981 tattgctatt gcaggaagcg ctatgcataa tctggcatta gctttacatc accttggtca
    14041 tgacgtgtcg ggaagtgacg atgaaatagt tgaaccagca aaaacaagat tagccaaggt
    14101 aggaatattg ccggagaaaa caggttggtt tcctgaaaaa atcaatgctg atattgatgc
    14161 cgttatttta ggtatgcacg cccgtgccga taaccctgaa cttgcagaag caattaggaa
    14221 aaagataaaa gtgttttcat atcctgaatt tttatatgag cattcaaaaa ataaaaccag
    14281 agttgtaatt ggtggcagcc atggaaaaac taccattact tcaatgattc tgcatgtttt
    14341 gaaaacatca ggaattaact ttgattttat ggtaggcgca cagctggaag gctttgacac
    14401 catggttaag ctatcaaacg atgcaaaggt tattatttta gagggagatg aatatctgtc
    14461 ttcgcctatt gacagacgac caaaatttca tttgtatcat gcccatatcg gactcatcag
    14521 tggtatagcc tgggatcata ttaacgtgtt tccttccctt gagatttatg aggaacaatt
    14581 cagaattttt gcaagagaaa ttccagatga cggaaaatta ttttattgtc ttaatgatgc
    14641 aactgttaaa actgtttgtg aagccgaaga tgtgaaagca ttaaaaactg gttacactat
    14701 tccaaaacac agcatcaaaa atggtaagac agtaatttac gatgacagga acaatgaggt
    14761 accacttcag atttttggtg agcataatct gatgaatctt gagggggcaa ggatggtatg
    14821 caactcaata ggtattagta acgatgaatt ttatactgca ataaaatctt ttaaaggcgc
    14881 agccagacga cttgagttgc tttcggagag taatcagttt gccgtcttta gagactttgc
    14941 acactcacct tcaaaactta aagccacatt agaagctgta cgcaagcaat ttccggagag
    15001 aaaacttatt gcctgcatgg aattacacac attcagcagt ttgaatgaga attttctgga
    15061 agaatataat ggtacaatgg atgaagcaga ggtggcagta gtttatttta atcccaaaac
    15121 aattgagcat aagaaattac ctccaattac ttctgaaaaa attttcaagt catttggcag
    15181 aagcgacata aagattatga atgactcaat acagttgatg aattatctga actcagaaga
    15241 taagcagcaa gtagtttatt taatgatgag ttcaggaacc tttgacggat tagatttaaa
    15301 atcttttgca gaaagattaa gcacctccca tcaaggtgtg taattaataa atatcaactt
    15361 ttcttttatt gccaaaggcc caacacaaaa acagaggcag ggctgccgac caggtcacct
    15421 gattatgttg accttcggga atttcaacat attgaatatt ttttgaagga actccttttt
    15481 gtttcatttc aacaatcaga tcgcgggcat catcaataga atctataaca ccatttttat
    15541 tcctgtctgc agtttcatct ttagtaccgg tctgaatcca acacttgatt gacggtgccg
    15601 atttactttt tcttataacc tgatgcataa ttctgtcatc atcagtataa ccatcttcat
    15661 acgatttttt tctccaccag aaactaccgc taaaaactcc tgctgtttca aaaacagcat
    15721 tatggttcca tgccaagtca aaagctgtta aaccactcaa tgaaaatcct gctattgcgg
    15781 tcttggatgg tgtaaattct atccctgtaa aactgctgca ctgtgcaatc agttcgtcaa
    15841 gtataaattt tgaaaattgt tttgctttat tacctctctt taaatagtca gccttacctg
    15901 caacaccgta ttcctgcaat ctgtttttat ttgcatgaat tgcaattact atgattggtt
    15961 taattttttt taattgcgtc aaatgctgaa gtgtatcttt tagttgcagc aattctgcat
    16021 cctgaccatc aagcataaac aatacccccg tgtttttagc gtattgtgaa ggcaaataat
    16081 agtcaattcg tacttcacgt tttaaaaact tacttggaaa tatggttgat attaaatcat
    16141 atttccactc tgacttaggt aatggcatag cttggtaaat gaaatatcag aaccaaccag
    16201 actttttacg tcttgttgca cctaaaccta atgctccaag taaacttctt gtaagtacac
    16261 tggtaacagt tctcatcact tgttttcctg tagagctgtt tagcacctga tctaactgac
    16321 taggctcttc ttttgcttcc gctcctttag ttgttttagt attttcggca acttgtgttt
    16381 ctaatttttt agaaagtact tcaaatgcac tttctctgtc aagtgtttca ttatattttg
    16441 ttgtcagacg cgatttagaa atactttttg ctaattcatc cggtgttaat atatccattc
    16501 gtgatgccgg tgtacaaagc aatgtatgcg caattggtgt tggcacacct ttttcattta
    16561 aaacagtgat tgccgcctca ccaataccca aagtagtaag caactcatca actttatagt
    16621 aatctgaaag cggataattt tctgcaataa gttttaaggc ttttctatcg tttgcagtaa
    16681 ataccctgaa ggcatgttgc acttttaatc ctaactgcgc caatacggca gccggtatat
    16741 cttccggtga ttgggtaaca aaaaatactc ctataccttt tgatcgaatc aactttatta
    16801 ctgtttcaat ctggtctaac aacactttag atgcattgtt aaatatcaaa tgcgcttcgt
    16861 caataaagat taccaatcgt ggtgcttccg ggtcaccaac ttcgggaaat gttgaatata
    16921 cctcagccaa taaactcaac atgaaggttg aaaataattt tggcttatcc tgaatgtcag
    16981 taagcctgat gatattcaat ttaccattgc cattttcgtc aattcctaac aagtcttcaa
    17041 cttcaaaaga tttttcacca aagaatttct cggctccctg ctgttctatt tcaataattt
    17101 ttcgaataat ggtagatgct gaagcgctac tgatggctcc ataatcggct ttaatttcat
    17161 cctttccttc attggttaaa tactgcaata cttttttgaa atctttcaag tcaagtaaag
    17221 gcaattgctt atcatcacaa tatttaaata tgagcgaaac aacgccttgc tgtgtatcat
    17281 ttaagtcaag aatttttgaa aaaagaacag ggccaaattc agaaactgtt gctctcaatc
    17341 gtacaccttt ttcatctgat aatgttaaaa actcaaccgg aaattgtaat ggcttccagg
    17401 taagtcctaa tttctgatag cgctcatcta tttttggatt tgttgttccg ggacttgcca
    17461 agccacttaa atctcctttt acatccatca ataaacatga aacaccttta gcagacagcg
    17521 attctgcaat tccctgcatg gttttggttt ttcctgtacc ggtagcacct gcaattaaac
    17581 catgtcggtt aaaagttttt aatggtgctt tgatgaaaca tcctgtcaac gtttcacctt
    17641 taagtaccgg tgcaccgagc acaatactat cgcctttaaa ctgatattct ttatttacga
    17701 cttctttaaa ctgttctgtt aagctcattt atgtatggtt aaaaactgct gacaaatgta
    17761 tgcctaaaag atgaaattaa acagaaaggt ttatttcttt gcaacttgtt aatgtatttg
    17821 acataatgcc acaatatatg cctgagggaa gttcagtttt tcaaagaatt tataaacagc
    17881 tatttctgat tttaaaatcg cttataacct tttcctatct gaaaatcata aagaacaagg
    17941 ctttaagagg tgttgccatt ttatttatgc tgatggttta ttatattgtt ctattgcata
    18001 taaactttct ttggctattt ggatatatgc ctaaaatgag tgaagtcaga aatccgcaag
    18061 tggctattgc tactgaaatt tataccgaag acagtgtgct gattggaaag tattatcatg
    18121 aaaaccgaac tcctgtgagg tttgattcaa tttcaactta tgcagtgcag gcacttattg
    18181 ccactgaaga tgtgcggttt tataaacatt tcggccttga tctttatgct ttaatcagtg
    18241 gcgcatattc cacagccaag ggcgatgaac gtggggccag taccataaca cagcagttag
    18301 ctaaaaatat gtacagcacc cgcaaaaaag tcaatcaagg tttgctgcaa catattcctc
    18361 ttgttggcac attggtaagt aaatcaaagg agtgggtaac atccgttaag ttagagatgt
    18421 tttattcaaa agatgagata ttggaaatgt actttaatac agttgatttt ggtaataatt
    18481 ggtatggtgt aaaggttgct gcacaaaact atttcagtaa acaaccatcg gaactcaatt
    18541 tgcaggaatc agctatgctt attggtttgc ttaaagctac aagtagttat aatcctttga
    18601 ataataagaa gcgctcatta accagaagaa atattgtttt gtcacagatg cagaagtatg
    18661 gatatatcag tcaggcaact tatgattcta tcagcccatt gcccttgaat cttaaacgcc
    18721 caaccaataa ccctgcaaca aaagattctt atttgagaca attagttgca aaaattatta
    18781 aaagttattg tgatgaaaat agcattaaca ttaatgaaga tggattgaaa atttatacaa
    18841 ccattaactc tcatctgcaa aactatgcag agttggctgt taaggatcat atgaaaaaag
    18901 tgcaaaaaca atttgatgag cattggggaa aacaaaaccc atggcgtgac gataatggaa
    18961 gagaaataat gaactttaca aatactgctt tggcatttac gcagtcatac gaccgtttga
    19021 aaaaacaatt tccttcatcg cctgattcta taacttatta tcttaataag aaaaaggtca
    19081 tgaaagtgtt ttcatacaat ggttcaatag atacagtgat gtcaaccata gattcgttga
    19141 aatactatgc tcgcttgtta aatgctggaa ccatgtcact gaatccactc accggtgaaa
    19201 ttaaagcata tgttggcgga gtggattttg atttttttaa atttgatcat gttgtgcaat
    19261 caaaacgtca gccgggttca acgtttaagc cgtttgcata tttagcagca attgaagata
    19321 cttttagtcc ttgcagcaga ttagacgatg taccggtatc tatagagtat gatggaggtc
    19381 aaatctggca gccttataat agtaccggtg catttactta ttcaagcaaa acattaagac
    19441 gggcattggc acagtcggtc aattcaatta ctgctcaaat tgccgaattg gttggatggg
    19501 ataaaattgt tgagtgcgca catagggcag gtgtaaagag ccgtttggat actgtgccgt
    19561 caatttgttt aggtacaagt gatgttagtg tttacgaaat gacctgtgct tatgcacctt
    19621 ttgtgaatgg gggtaagagt gttgaaccca ttttagtgaa gagaatttgt gcaaacgatg
    19681 gaactttgct caaggaattt gttccagaat tcaagcaagt aataacagaa gagaatgcat
    19741 ggttaatgtt atatatgttg cagggtacga ttcaggaatc tggaggaaca tctcaggcat
    19801 tatggggcta ttctattttt aaaaatggga atgatgttgc aggtaaaaca ggaactactt
    19861 caaattattc tgatgcatgg tatatgggcg ttactcatga tttagttacc ggtgtttggg
    19921 ttggtgctcc ttatcgaagt gtgcatttca gaaattcatc aggtcaggga tcgcgactgg
    19981 cacttcctat attcggaaaa atgttagaga aagcttatca ggatcccaag tcaggtgttg
    20041 ttcttggaaa gttcaaaaag ccaaagaaaa tttctaaaac aatatattgt gataatgatg
    20101 atattgaact tgaagcagca gattcattgt taaacgattc attaataaac accgaattga
    20161 tgattcttga aaatgactct gtaaagtcag attcattaca aggtggtaga gattaaaaag
    20221 aattttattt tagcagaaaa aatatgcgta gaaaaattgt agcaggaaac tggaaaatga
    20281 atggtacatt agatgatgcc atcaaactga catctgaaat tgcaggaatg tataaggatg
    20341 aaattaattc tgatgtacag gttgtattgt caccgccatt tccgtttttg aatagtgtta
    20401 gtaaactcac taataatact gcaattaaag ttgctgcaca aaattgtgcg gcacaggctt
    20461 ctggtgcttt tacaggtgaa gtttctgcct ctatgattac ttcatgtggt gccggatatg
    20521 ttattcttgg gcacagtgaa agaaggacgt atttttctga caccgatgaa gtactgttgt
    20581 taaaagtaag acaggcatta ctgaatggac ttcaggtaat tttctgtatt ggagaaacaa
    20641 aggcagaaag agaaagtaat agccattttg actgtgtaga aaagcaaata ttaaatgtac
    20701 tctcacaact aacgcctgac gaatttgcga agtgtattgt tgcttacgag cctgtttggg
    20761 caattgggac aggcctaaca gcatcacctg cgcaagttca ggaaatgcat cactttatca
    20821 gagaagtttt gaagaaacaa tttggtgaac aggcctctga aacttcaatt ttatatggag
    20881 gaagttgtaa tgaaaacaat gcaaaagaac tgtttgcctt acctgatgtt gatggtggat
    20941 taattggagg ggcatcttta aaatccagaa gttttatttc gattataaaa tcattttaac
    21001 tgaatgagta attcattcaa aagtgtttta ctttttgcat ttttggtttg cagcttattt
    21061 tctgaagcac aatcattaag tcaacctgca tttgttgaat atagtttcag tactggtagg
    21121 gtagttaaaa actatcctgt ttttcctgaa agaaaattag caggagtcaa cagttttata
    21181 ataggcagca gattaaatgg cattaaggca tggcatcctt attataattt tcctaatgca
    21241 tcattcgaac ttgcttatgg cagtttaggt agtaataaag ttctcggaaa tttctctgca
    21301 ttgctttaca ggttagaatt cggttacaag ttgaatgatc aattttatac agaaattacg
    21361 cctgcatttg gtattgcata ttttaataaa ccatttcatg cagataagaa tcctgagaat
    21421 acactcatag gttcagcatt tacattttgt gcggcagcgc ggctcaacct gcgatattat
    21481 atcaatccat tttggtctgt cagtgcaggt gcaggtgttt atcattgctc caattcgcat
    21541 tatcaattgc ctaacctggg tattaacttg ccatttgcat cagcaggact tcgttatcat
    21601 atcaggccgc ttaatttgct tttgggcagt cgcagtattg agaaggattc cggctatcat
    21661 tttcaagtac ggtttgctgt tggatataac gaacaagggg caagtacatt tcctgtgaat
    21721 ggtccaagat atccaatata tcttggagca ttttatgtga ctcgttatct gactccggta
    21781 aataaattac atttaggcgt agaaggctgg tataacagtg gcgtttatga ttttatcacc
    21841 agtcagcgat attacaatga taaagagcat ttacgttcaa cagcatggca ggcagtagtt
    21901 ggcaatgaat ttttatttgg tcattttagt atgctgataa atggtggtat atatttctat
    21961 aatcccgttt atcatgagaa gttaagaaga gaaaacgaaa caaattttaa aaagcaatta
    22021 aaaggttgga taacggcaag actcggtttt caatattatc ttaaagatgc taccatctat
    22081 cacaaaagta atttttatgc tggtgtttac ataaagacca accttggcca ggccgatttt
    22141 cttgaaaccg gtattggtta tgccttttag tttttaatat gcaacttgaa taactttaga
    22201 aatacagatg aatattctta agtaatattt tgtatagttg catcataaca gaaaaaatga
    22261 aaaacagaat tacagagttg tttggaattg aatatcctat tgtgcaggca ggtatgattt
    22321 ggtgcagtgg ttggagactg gcttcagcgg tcagcaatgc cggtgggctt ggtttaattg
    22381 gtgcaggaag tatgtatccg gatgtattaa aagagcatat tgaaaaatgc aaggtagcca
    22441 ccaacaaacc ttttggtgta aatgttccat tgttatatcc tgatatagag aaactgatgg
    22501 caattattgt tgcaggtgga gtgaaaatag tttttacttc tgcgggtaat cctgccactt
    22561 ggactaatta tttaaaacaa gcagggataa ctgtggtgca tgttgtgagt aatgtaaaat
    22621 ttgccttaaa gtcgcaagcc gcaggtgttg atgctgttgt tgcagaaggt tttgaggccg
    22681 gtggtcataa tggtcgtgaa gaaacgacta caatgtgttt gattccggag gtaaaagatg
    22741 ctttacagat tccggtgatt gcagcaggag gaattggtag tggtcgtgct atgatggctg
    22801 catttgcatt aggtgcagaa ggtgtgcagg ttgggtcacg ttttgtagcc agcgtggagt
    22861 catcggcaca tactaacttt aaagacaagg ttattaatac atcagagggg caaacagttt
    22921 taacattgaa acaactgaca ccggtgagac tgataaaaaa taaatttagt gaacaagttg
    22981 aacaggcgca acagcgctgt gcaacgcgcg aggaattgac gcagttgctc ggccgtgcaa
    23041 gagcaaagaa gggaatgttt gaaggagacc tcaatgaagg agaattggaa attggtcagg
    23101 tgtcgtcaca aataaaaaat attattcctg cccgcgacat tgtgaaaaat atttggaatg
    23161 aatttttaaa agaaaaaaac cgaattgcta atttgtttta aattatgatg aaatttgaaa
    23221 agatagtttt acccaatggt ttgcctgtga ttgtaaatta tgatccttca acacctttgg
    23281 tagcaatgaa catattgtac aaagttggtg cacgcaatga agatgcaggc cgaaccggtt
    23341 ttgcgcattt gtttgaacat ttaatgtttg gggggtcttt aaatatccct tcttatgatg
    23401 aacctttgca gcgtgccgga ggagagaata atgcatttac caacaatgat tttaccaact
    23461 attatctgac tatcccttcg cagaatgttg aaacagcttt ttggttagag agcgaccgca
    23521 tgttaagtct tgcattctca gaaaaaagtt tagatgtgca gcgcaatgtg gtaattgaag
    23581 aatttaaaca acgctatttg aatcaacctt atggtgatgt gtggctcaat ttgagaccac
    23641 ttgcatacaa agttcatccc tatcaatggg caacaatagg taaagacatc aaacatattg
    23701 aagatgctac actggatgat gtaaaagctt tttttcataa atggtattgt cctaacaatg
    23761 caatcctcac cttatcagga aatattaaac cggaatacgc ttttaagctt tcagaaaaat
    23821 ggtttggcga aataaatgca catgatttat ctaaggagag tttgccaaaa gaacctaagc
    23881 aaaatgagta cagaaatatt attttagaaa gagatattcc tgcaaattct atttacaaag
    23941 ctttccacac ttgttcgcga tttgatgatg agttttatgc tacagaatta ctcaccgata
    24001 tactgtgtgg aagcaaatcg tctttgttat atcatacttt agttaaagaa caacaactgt
    24061 ttaattcaat caatgcttat tttacaggtg acttggataa tggattgatg gtagttgaag
    24121 gaaaagtaaa tgatggcgtt gaagtaaaaa atgcagatca tgctgttgat gttttgttgc
    24181 aacaagtagc tgatgatggt gttcaggaga aagaaatgaa gattgtaaaa caaaaaacag
    24241 aatctgcgtt aattttttcg gaggtaaata ttggtaacag agcattaaat cttgcatatt
    24301 ttgagatgat aggtggtgca gaaaaaataa ataaacagtt acagcaatat gaggctgtta
    24361 attcaaatga tgtacagcaa gtggcaaaaa caattttaaa aaaagaaaat gaatcttcaa
    24421 tttattattg caagaaagga gtcactttat gaatagggta gcagagcctg cgttgacatt
    24481 aattagtaag attgatatta cattgccgga aaagattgtt ctggacaatg gcatacaagt
    24541 atttgttctt aatgctggag aacaggaagt aactaagctc gacatagttt tcagggcagg
    24601 ggattggtat ggtgaattta gtacattagc tgctaatgtg aatgagttac tggattccgg
    24661 aacagcaaaa cgtacagcct atgaaatttc gcagtcgttt gaccaatatg gagcctatct
    24721 gaataccact tgtaattttg atacagcatc agtaaaactt tttactttat cacgatttat
    24781 tcctgaaact ttttctttgt tacttgaaat tatttcagag gcaatattcc ctgatgaaga
    24841 agtgaaaata ttaagagatc aaagtattca gcaattaaaa atcaatagtc aaaaggttaa
    24901 ttatatttca agaaataatt ttaacaaaat gttatttccg ggtgatcatc cttatggcag
    24961 gtcatcttca gtagcagatt atcagaagtt gaattctgca gatttacttt tatggcataa
    25021 tcaatattat caacctcaaa actgtttgat ttttcttagt gggcgtttta atgaggatgc
    25081 tattaaatct ttaaatcaaa tatttggaaa gtggaaagat acttcaccgg gtaaaataat
    25141 tgagaaaaac ttttctgttg aaaattataa gcctgaaaaa aagtatattg aaaaaaatga
    25201 tgcattacaa actgctattc ggattggatg tcatgcccct aacaaattgc atcccgacta
    25261 tattccatta acagttttat caacagtttt aggcgggtat tttggctcaa ggctgatgag
    25321 taatttaagg gaggaacgtg gttataccta tggagttggt tgtacattag tatcttggct
    25381 tcatgacgga tacttcttaa tctcaaccca agtgggtaaa caacataggg agcgtgcaat
    25441 agaacagatt tatcttgaaa ttaaaagact tcttgaagaa aaaatctctg acaatgagct
    25501 taatcgcgta aaaaattata tgacaggtgt ttatcaaaga agtttagacg gtccaatgct
    25561 tgtgtctgat agaataagat ctacggtttt atacaatcag gatttcaatg ttttaaatac
    25621 atatttacaa atcatgaatt ctacaaattc tgaccagctt attgaattgg cgaatcgtta
    25681 tttttctaat gaaatgactg aagtttctgt gggttaagtt aaatgagttt ttacggtgac
    25741 aaccctgtta aaaaaatatc gtttaaatat taaatttatg atgtagaaat gaagaatagt
    25801 tttatatttg tgcaaacaaa tttaatgaag ctatgatacg aaaacacatt
//
LOCUS       DAOQNP010000040        25304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_654_length_25304_cov_9.432928, whole genome shotgun sequence.
ACCESSION   DAOQNP010000040 DAOQNP010000000
VERSION     DAOQNP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25304
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_654_length_25304_cov_9.432928"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKY09_07000"
     CDS             complement(<1..799)
                     /locus_tag="PKY09_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12642.1"
                     /translation="MINRSNYEVWFIDYADGQLSPEQVAELLLFLEENPDLKNEFTLF
                     EQVELPVDTVEFPFKKSLKKTKPSKEVFDEMAVRFLENEILPSESELYQQWINDFGDL
                     KAEHSLFALTKLHADANVVFRNKNRLKKKTGSVVLMPWIRYVAAACVVTMVLFFANRN
                     KPVNQQQAENKTNSSPVQHNTPPIKVDVIPDTKSTIALSSKKNNVETVRILEHSKQKS
                     EAKESWKYPSVEIALITAKSGVLTPSVKSEPVPVANIENSYAMVMKNT"
     gene            complement(802..1284)
                     /locus_tag="PKY09_07005"
     CDS             complement(802..1284)
                     /locus_tag="PKY09_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB12643.1"
                     /translation="MTALEYNTCVDQYADGIYRFILHNIKNEDDARDVVQDTFEKCWR
                     NHENVQYEKAKSYLFTTAHHTMIDRIRKSSHQELLSEYHEEPSHSKHYSDLKNVLKLA
                     LDKLPAIQKSVILLRDYEGYDYKEIGEITGLSESQVKVYIYRGRVALKEFIGSLSNVI
                     "
     gene            1441..2070
                     /locus_tag="PKY09_07010"
     CDS             1441..2070
                     /locus_tag="PKY09_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12644.1"
                     /translation="MNKLFTVFIFLLCNNAMNAQKTLNGIYRLHGIQDMAAAFQFLPD
                     STFEFFYSYGAVDRKAKGTYTYDGDTIKLNSDKIGGADFEITEQRISGKTYKVVVRDE
                     NQYLTQKVRAIVTFGNLTKEYEANEDGVINIDTKNCEKIFLVHELYPDVASTIKDELN
                     ANTYFEVRLKPSLEDVSFKGINLFKNGENFKCLPNYFLPFSNILFIKED"
     gene            2172..4241
                     /locus_tag="PKY09_07015"
     CDS             2172..4241
                     /locus_tag="PKY09_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNB12645.1"
                     /translation="MRILLVILTATAGVLALTSCKQTPKQENTTPIAKTTPEVEIVAD
                     RFADIQMLRYTIPGFEELSLQQKQLAYYLYMAGMSGRDIFYDQKNRYNLQIRKTIETI
                     LETYQGDKNEDNYKKFVVYAKRFFFSNGIHHHYSTDKMIPEFDPAFLDTLISKSDFNK
                     MPNEGKNIEEYIAFLKPILFDPKIDAKGVSLAADADNVKASSSNLYEGVTQKEVEQFY
                     KSKIQPGVKEQPSWGLNSKVVKINGKVEEQVWKSGGMYGAAIDKIIYWLEKAVTVAEN
                     DAQKATLEALVKFYKTGNLKDYDDYCVLWVKDTASRIDIANGFIEVYLDPMHKKGAYE
                     SVLSLKDLEATKRIAAIGKEAQWFEDHSPILPEHKKKSVKGISAKVITVINECGDAAP
                     STPIGINLPNQEWIREKVGSKSVSLGNIVASYNYARAKSPMIDEFGENPQVIERVKKY
                     GALAGDLHTDMHEVIGHASGKINDGVANTDVTLKNYASTLEEARADLVGLYYITDKKL
                     IDIGVMPTIEVGKAEYDSYILNGLMTQLNRINPGDNLEESHMRNRQLVASWVYEKGKE
                     ANVIERITRNGKTYFKINDYDKLRTLFGELLKEIQRIKSEGDYTSGKSLVENFGVKVD
                     QDLLKEVHERYKALNIAPYMGFIQPKLVPVMQGDSITDVKVEYNNDFIPQMLEYGKEF
                     SFLPIKN"
     gene            complement(4238..5293)
                     /locus_tag="PKY09_07020"
     CDS             complement(4238..5293)
                     /locus_tag="PKY09_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNB12646.1"
                     /translation="MSQTENIINTFIRVGQRIKEHCEQYPNGQLKDIVATAKAKNPWF
                     TAHNQINALNAIAYMLQPDSLQQWISCYSISEHLPKNIGVVMAGNIPAVGFHDLMTVL
                     VSGNKIQIKPSSSDSILMEWITGIIKSENAELGNRISFVDKLQKPDAVIATGSDNTAR
                     YFHYYFSKVPHVIRKNRNSVAVLNGNETKEQLFALGEDIFSYFGLGCRNVSKLMVPEN
                     YNFEKFFDAIEPYGNELLLHNKYINNYDYYRAIYLMENIPFLTNNFVHLLEQQNIASP
                     VSVLHYETYNNQSSLHQLLTAEADKIQCIISADVITGKEINFGMGQQPSASDYADGVD
                     TLRFLIEQCSEQQNSNT"
     gene            5409..5756
                     /locus_tag="PKY09_07025"
     CDS             5409..5756
                     /locus_tag="PKY09_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNB12647.1"
                     /translation="MAIIITDECINCGACEPECPNNAIYEGGAEWRFSDGTSVKGMFN
                     KMDGTAIDADAAQAPVNLDHYYIVPDKCTECVGFHDEPQCASVCPVDCCVDDENVRES
                     KDELLAKKSRMHQ"
     gene            5830..6705
                     /locus_tag="PKY09_07030"
     CDS             5830..6705
                     /locus_tag="PKY09_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12648.1"
                     /translation="MFRTAITFFILFFATGNTFAQLQLFADSVIKGSSDSVRQQYQLK
                     LTHAMNNFLATEHSFSTSFDTIPSISTVISSDGLIRFYQWVQPNLETKTYSIYGMMQV
                     KDKKTKTIKTYTLTDGAMDKYTASDKILNTDNYFAAIYYSIISLKENKKTIYTLLGWR
                     GVDNRTTVKTIDVLHFQKNKPVFGKKIFKAPANMLPVMSAEKCTRVIFQYNAQAVMSL
                     KYYSKGKKIVFDHLSPPKATLKGAEETYGPDFTYDAFIWKKGKWQGKSDVDIRSSHNT
                     DGQKITPVKDSELRK"
     gene            6775..7839
                     /gene="serC"
                     /locus_tag="PKY09_07035"
     CDS             6775..7839
                     /gene="serC"
                     /locus_tag="PKY09_07035"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010422638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNB12649.1"
                     /translation="MRKHNFGAGPGVLPEAAIKKTQESLFDFNNSGMGVMEISHRSKD
                     FDAVLQKAKSLVKELLHVPDTHEVLFLQGGASTQFAMVPMNFLKKKAAYIDQGAWSNK
                     AIKEAKLFGEVEVIASSKDKNYSYQPKGYTIPADCDYLHICSNETIHGTQVKNFPASS
                     IPVICDMSSDIFSRPVDVSKFDLIYAGAQKNMGPSGVTLAIIKKDIVGKSERVMPTML
                     NYKTHLENDSLYNTPPVIAILVCMHVLEWLKSIGGVEAMQKINQQKFDMFYNEVDSNP
                     LFTGTAAAEDRSPMNACFLLKDASMDEAFLAKAKEAGIVGIKGHRSVGGFRASMYNAL
                     PVESVKVLVQVMKDFAKANG"
     gene            7858..8829
                     /locus_tag="PKY09_07040"
     CDS             7858..8829
                     /locus_tag="PKY09_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB12650.1"
                     /translation="MIKILANDGIDAAGKKIFDAAGFTVSTDKVPQEKLAEALKNFDA
                     ILVRSATTVRTDVIDACPNLKVIGRAGVGMDNIDVKYARDKGLAVINTPAASSQSVAE
                     LVFAHLFTGVRMLYDANRNMPACSTKETFDKLKKSYSAGIELKGKTIGIIGAGQIGKC
                     VAAIAVGLGMNVLFHRLNNEDVVVDMDFHPALGQKKITLTFKCVSFTKLLIESDFITM
                     HVPFPKGSAPVIGKKELDLMKNGVGIVNTARGGVISEVDLIEALNSGKVAFAGIDVFE
                     GEPLINQTLCTHPKVTLSPHIGGSTKEAQERIGIELAEKVVEYFTKK"
     gene            8857..10101
                     /locus_tag="PKY09_07045"
     CDS             8857..10101
                     /locus_tag="PKY09_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNB12651.1"
                     /translation="MAVLKPFKGIRPKKELVKRIACRPYDVLDEKEARAETKGDKISF
                     YHVIKPEIDFPDDFDHYAPEIYKKGKENFEAMFKDGVFFQDSKDCYYIYAQSMDGRKQ
                     YGIVGCAAVDDYVNDVIKKHELTRPDKEEDRKNHVRVSGLNYEPVFFAYPQVRKLDEL
                     VEHITAAAPEYDFVTDDHITHQFWIVDNDSDINIITEEFAKLKNTYVADGHHRTAAAA
                     LVGKERREKNPSHTGKEEYNFFLAVHFPDNQLKIMDYNRVIKDLNGMNAVQFIDKLKE
                     NFIVEEKGSDAYRPAALHEFSMYLEGKWYKLNAKPGTFNDKDPIGVLDVTILSDKVLA
                     PMLNITDLRKDKRIDFVGGLRGLGELKRRVDNGEMKVAFALYPVTMKQLIDIADNNLI
                     MPPKVTWFEPKLRSGLVLHSLD"
     gene            10217..11629
                     /locus_tag="PKY09_07050"
     CDS             10217..11629
                     /locus_tag="PKY09_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12652.1"
                     /translation="MMHDDDYEGFDDQSENLGNSVQRFEEMLRNHETYFFDADSLIKI
                     TDFYLEKFDYDKAMEVIKYGQNLHPDNTAFKAKEAYISHLQGNSEEALEMIDQLLTVN
                     PKNEDLYLIKGLIYNALGMFQEGRKTFKDLLSFTEFKEDVYFHVAQTYLQTFDYHGAI
                     HYFKNCLFENKKNEVAADELSECFDRAGRASEGITYFQALIDQDPYNALFWFHLGQMY
                     QCCAEFEKAINAYDYCILIDENMDDAYIQSGQCHMLAGNYEKAIQAFKQSFEYNTPEA
                     FVYDSIAQCYFELGNHEESRSYFKKAVKSNPSFHSSWYGIGMTLSEEERWYEAIHYLK
                     KAIELNGDISDYWFLLGDCERELGNLEEADEAYRKVIEMDPDFLDIWYEYPEFLMEQN
                     RTDEALQYAYLAVKYFPNDIAVHLRYIILQYRNGNIKTAYSLLSAAANNEENIREIIE
                     EIYPELLNDSNIQKILEQNN"
     gene            11635..12420
                     /locus_tag="PKY09_07055"
     CDS             11635..12420
                     /locus_tag="PKY09_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HNB12653.1"
                     /translation="MNFELSQMPSRSTKPRENGLTMVMDKGLSLRETEDFISSSGEFT
                     DIVKLGFGSSLITPNLDKKIALYKEAGIPVYFGGTLLEAFLIRDEFDTYLKVLSKYKM
                     EYAEISDGSIVIPHKIKCDYIKKLAKHVTVISEVGSKEEGIIIRPNVWIQMMERELEA
                     GVWKVIAEARESGTVGIYRPNGKAHVMLINKIISRVPADKIIWETPQKSQQVYFIKQF
                     GTNVNLGNIAPNEVIALESLRLGLRSDTFFHFLDKETINKFTN"
     gene            12623..13810
                     /locus_tag="PKY09_07060"
     CDS             12623..13810
                     /locus_tag="PKY09_07060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNB12654.1"
                     /translation="MNRGNSKTNLNALACNLYINGEFSLVQGIPSVDYEPLYPALMSI
                     CYAITGYNWLGLTILQATLHLITSLLLFKIGSRLFHPIAGFFAACYHALYPYLFTYSF
                     SIYDTTLFVFLLVLTIDLLLTEKLSSKRLVLAGIIIGLTFLTRATIITFLPGLFLYVF
                     YRYFKSQNITAAFISCSLIVIGCFLAMSPWLVRNYSLTGKLLISTHGSFGMWQGNNPL
                     SFEYLKANKSLDEIYRLNPPVPIYAQYPLTARTPDEAVKVAAAYQQEAINWIKNNFPE
                     FVSMGWLKAEKLWSWNRNPDSKSVKFGSNEGRKSVYLISYLPLLLLFPFGLILLFKKH
                     PAEAFTLLLILLAFTGAHAIAIGFTRARIPIDFFLMLCSGITITWLLQRFFPSLYLKF
                     KSS"
     gene            13807..14916
                     /locus_tag="PKY09_07065"
     CDS             13807..14916
                     /locus_tag="PKY09_07065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB12655.1"
                     /translation="MSDKKYTVIFLGKLPPPYIGPSVACEIILNSKLKDEFNLIHLDL
                     SDKRDINTLAKLDFTNFYLAFKQYFQLFWYCIRYRPNLVYVPAGQTNVGYTRDAGYIL
                     IAKLFGIKVLTHLRGGYFLNWYNESGKTMKSFIRFVHKKVNGQIVLGNNLRKLFNWIV
                     PDEKIFVIPNGGNFPVNYNERNNNKVKVLFLGNYVRTKGVLEALYATPLVYEKNKNVE
                     FVFAGEWREEDVKTDFENFLQQHPELPVVNHGPVKGDKKFSILNEADIFVFPTYYRNE
                     GHPWVTVEAMAAGLPVISTDHAAISESVHDGKNGYLVEKKNIQQLAEKIIILANDKEL
                     RNKMGAESRRIYLENLTEEKMVERMGHAFRSVIEN"
     gene            14928..15887
                     /locus_tag="PKY09_07070"
     CDS             14928..15887
                     /locus_tag="PKY09_07070"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNB12656.1"
                     /translation="MKKVLVTGGAGFIGSNLCDALLANDQYEVHCLDVFDDFYNPALK
                     RNNILEALKSKNFFLHEGDIRNTTFLEKLFQQPFDIIVHLAARAGVRPSIEKPLLYED
                     VNIKGTINLLEKAKEKNIKQFVFAGSSSVYGINPNTPWSENDFVLKPISPYASTKVAC
                     ELTGHSYSHLYGIRFISLRFFTVFGPRQRPDLAIHKFFKLIRQEKSIPFFGNGETSRD
                     YTYVSDIVKGIIAAMHYDKSNYEIINLGNNRTINLNMLIAAIEKEAGKKAILEKLPDQ
                     PGDVPHTFADISKAKELLNYSPATTLEEGLTAFRKWFDQNYSA"
     gene            15907..18033
                     /locus_tag="PKY09_07075"
     CDS             15907..18033
                     /locus_tag="PKY09_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNB12657.1"
                     /translation="MEQLTQNLKDGKMQLLEVPFPVLSSGQVLVRNHYSLISAGTEGK
                     TVKDARLSYIGKARARKEEVKKVIDAAKTFGLLNTYKMVMNKLDAPSSLGYSSAGEII
                     AVAPDVTEFRVGDLVACGGNSAVHAEVIAVPVNLCVKLNDKIALQHACFTTLGSIALQ
                     GIRQADLRLGENCVVIGLGLVGQLTVQMLKAGGIKTIGIDIDQRMVDLALHTDIDAAF
                     TRNSEDLESSINNITNGYGTDAVIITAGTDSLDPVDLAGALCRRKGKVVIVGAVPTGF
                     KRPNYFKKELDLRMSCSYGPGRYDSEYEEGGIDYPYGYVRWTENRNMAAFVDLIATGK
                     INLTNLLTHTFDFKEAPAAYDLIMNKTEPFVGMVLKYDTHKPLQQKVNLSNPSYTKSD
                     VVAGMIGAGSFGQNFLLPALKNTPAKLSGIVTARPNNARNIADKNQFAFASGNAQDIF
                     SNGDINTVFIATRHETHAGYVMQALQSGKNVFVEKPLCLSEEELVQIQHEFTKHNCRV
                     MVGFNRRFAPMIQTIKKTFSSGNPIAINYRINAGVIPADHWVHDLKVGGGRIVGEACH
                     FIDLCMFLAGSKLTTISAVALNDAQKLNDTVCFNLQFENGSTASVSYFSNGNKNISKE
                     YLEVFGSGIVAVLDDFKTLTVFGKSEKKYTGNQDKGHQNEVKAFVESIIQGKPAPIPF
                     EDTVLSTLATFRALQSIASNGERIIL"
     gene            18038..19210
                     /locus_tag="PKY09_07080"
     CDS             18038..19210
                     /locus_tag="PKY09_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-aminolevulinic acid dehydratase"
                     /protein_id="HNB12658.1"
                     /translation="MDASRTIEAATSQLRKYIEAHRYKGYDPYDALKSPLFKLPLLNK
                     NKLIRFGAQQLVKRSPLNLRTLLFVPKGLNPVTLGLCIQAYAQLIHVNQQEKNVLNQN
                     ISNLINELEKLIPKGFHGACWGYDFDWEARYANIPAYQPTVVATGIITNALFIAWQQT
                     GNQKALDLCKSAANFVLQDLNRTTDSDGSFCFSYSPFDKQVVFNASMKGSRLLSQVYS
                     VTKDETLKKAAADSVAFVMKHQRADGAWIYSTSDAGTWIDNYHTGYVLDCLHEYILHC
                     DDKTYNENLEAGKKFYINNFFLEGHIPKFYNNNVFPVDCTAAAQSLLTLTRFDEQTLA
                     IKVAEYMLENMQHSKGYFYFRKYKNYTEKTSFMRWSNAWMFAGLTAVLQSQCAHRQ"
     gene            19391..20440
                     /gene="lpxD"
                     /locus_tag="PKY09_07085"
     CDS             19391..20440
                     /gene="lpxD"
                     /locus_tag="PKY09_07085"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013552858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNB12659.1"
                     /translation="MKFTAKQIADLLKGTVEGNHDAEVNSLSKIEEGKPGTLSFLSNP
                     KYTPYIYDTKASVVIVNKDFVAENTINATLIRVEDAYKSFASLLEIYNQIVNDKKGVE
                     SPSFIDPSATVGDQGYIGAFAYIGKNAIIGKNVKIYPHCYVGDHVIIGDNTTLFPGVR
                     VYSFCHIGSHCNIHAGVIIGADGFGFAPNENNNYKKVPQIGNVVIHDHVEIGAGTTID
                     RATLGSTVIHKGVKLDNLIQIAHNVEIGENTVIAAQTGVAGSTKIGKNCMIGGQVGII
                     GHLAIADGVKIAAQSGIGTSITTPNEIVQGSPAFNISDYKRTYVVFKKLPQLERKINE
                     LEKKTGSLNPAENKS"
     gene            20437..21831
                     /locus_tag="PKY09_07090"
     CDS             20437..21831
                     /locus_tag="PKY09_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNB12660.1"
                     /translation="MSVKQVTLKEEITVEGPGLHTGVNARLTLKPAPENHGYKFKRTD
                     VNGQPVIDADVDNVVDTSRGTTLSANGVKVSTTEHVLAALVGMEVDNVLIEIDGPEVP
                     ILDGSAFPFVNAIQKAGLREQEATRSYFVITENLTYEEPARNVEMLAVPSPDYRITVM
                     VDYNSPVLGTQHAQMHKITEFKDEISKCRTFVFLHELEMLLKHDLIKGGDLNNAIVIV
                     DREIKQDKLDELATLFKKPKVEIKHKGILNNTELHFQNEPARHKLLDIVGDFALLGVH
                     LKGHILAARPGHAANVAFAKQFKEIIKKDKNRLKAPIIDPNKPALFDINRIESFLPHR
                     FPFLMIDKITELTETGVVGVKNVTANEWFFQGHFPGNPVMPGVLLIEAMAQTGGILVL
                     NTVPDPGNYWTYFLKIESARFKNMVKPGDTVVFKLDLISPIRRGLCHMGGQAFVGDKV
                     VMEAEMMAQIVKKS"
     assembly_gap    21837..21936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21937..22188
                     /locus_tag="PKY09_07095"
     CDS             <21937..22188
                     /locus_tag="PKY09_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HNB12661.1"
                     /translation="EPLSYIGINNIGLRRRGFTNEDINHIQDIYRILFVQNSNIKKAL
                     TEIEKEIPESTLRSQIVEFIHNSPKGLMRGFTPSDNDDE"
     gene            22211..22837
                     /locus_tag="PKY09_07100"
     CDS             22211..22837
                     /locus_tag="PKY09_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB12662.1"
                     /translation="MFTVNLTEAGKKFKQHWIFRKCSASFSSGDVIGITGKNGSGKST
                     LLQVISGYVSPNEGKIEWNEHDKKIEADAVYRYLSIASPYLMLIEDFTLEEMIGYYFR
                     FKNLRPSFNITSVITESGLEAHRNKLLSEFSSGMKQRTKLLLAIASDTPLLLLDEPCT
                     NLDKQNITWYQQFLKTHSEGRLIFICSNHIDDELFLCNKWLSVEDFKF"
     gene            23058..23234
                     /locus_tag="PKY09_07105"
     CDS             23058..23234
                     /locus_tag="PKY09_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12663.1"
                     /translation="MMNNDPIITYDHTQEESNFRSRYFKKSLFGKKNTMLLFFAEEVL
                     ILGFLAYLVYLVFV"
     gene            complement(23276..24874)
                     /locus_tag="PKY09_07110"
     CDS             complement(23276..24874)
                     /locus_tag="PKY09_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12664.1"
                     /translation="MPLIGHTQNWQWAKHIGSAYQFYGEEANVITDGINIYLVGSYGG
                     TLYLPGDTLYSNGNNDIFISKFDASGNLVWAKTLGGNYMQPNNHEYARGVFDSICNCI
                     YVTGNFKNTVDFGNGITLTSLGTQTDNFLTRMDTSGNFLWARAMGGIGEERLPYINVN
                     ANGKIYLLTQTADSSHFGSFFIPPGGGIVQYDSSGNCLSAEIKFSAPVTGNTNGVFLD
                     FIGADLILYGLYRSTPFQLDTATLITQGNYDGFIARADSNGNVKWIKTYGNAGLDYIQ
                     DLTIDNSYNLYITGGFQDSINLGGNTIQANSSDIFIAKLDAVGNFIWNTQLSVTGNYK
                     SPNNIFSDADGNFYLAGLFSGNANFGTFNINTSNTYDMFISRFNNNGDCLGVRHFGQA
                     SGSSVAVDNSGSAYCAGAFDNTINIGSTSMTALLGQDIYLAKIDAITGIGGGEGRMAN
                     NQMLIYANPNAGKCNITVPDDFVNEKNLTLSIYDNTGKLIQQKTLEMNDGNIKLNLEQ
                     EAKGVYNIVLSNKQKSYNGKIVFE"
     gene            complement(24946..>25304)
                     /locus_tag="PKY09_07115"
     CDS             complement(24946..>25304)
                     /locus_tag="PKY09_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12665.1"
                     /translation="INIGSTSMTALLGQDIYLAKIDAITGIGGGEGRMANNQLLIYAN
                     PNAGKCNITVPDDFVNEKNLVLSIYDNTGKLIQQKTLEMNDGNIKLNLEQEAKGVYNV
                     TLANKKKSYSGKIVFE"
BASE COUNT         8258 a         4576 c         4665 g         7705 t
ORIGIN      
        1 ctgtgttttt catcaccatt gcataagagt tttcaatatt agcaacaggc actggttcag
       61 atttaactga tggcgtcaat actcctgatt ttgctgttat cagtgcaatt tctactgatg
      121 gatacttcca actttctttt gcttcagatt tttgtttact gtgttctaaa attctaaccg
      181 tctcaacatt attcttcttt gatgataaag ctatcgtact cttagtatca ggtataacat
      241 caacctttat tggtggtgta ttgtgttgta ccgggctact attagtttta ttctctgcct
      301 gttgttgatt aaccggctta tttctattgg caaaaaacaa caccattgta acaacacatg
      361 cagcagcaac ataacgaatc cacggcatta aaactacact accggttttc ttcttcaacc
      421 tattcttatt ccgaaaaaca acattcgcat cagcatgtaa ctttgttaga gcaaataaac
      481 tatgctcagc ttttaaatcg ccaaagtcat ttatccattg ctgatatagc tctgattcag
      541 atggcaaaat ttcattctcc aaaaaacgaa ccgccatttc atcaaaaacc tccttagaag
      601 gctttgtttt ttttaaactt tttttgaaag gaaactcaac cgtatcaaca ggtaattcta
      661 cttgctcaaa taaggtaaac tcatttttaa gatcaggatt ttcttcaaga aaaagtaaca
      721 gttcagcaac ctgctcgggg ctgagttgcc cgtcagcata atcaataaac caaacttcgt
      781 agttgcttct gttaatcatt tctaaatcac attactcaaa cttcctataa actctttcaa
      841 tgctactcta cctctgtaaa tataaacctt cacttgcgat tcactaagtc ctgttatctc
      901 gccaatttcc ttataatcat aaccctcgta gtcgcgcagt aaaatcactg acttttgtat
      961 ggccggcaat ttatccaaag ccagtttcaa cacatttttc aaatcagaat aatgtttgct
     1021 gtgcgaaggc tcctcatgat attcactcaa taattcctga tggctcgatt ttctgattct
     1081 gtcaatcata gtatggtgag cagtggtaaa taaataagat tttgcctttt catattgaac
     1141 attctcatga ttacgccagc atttttcaaa ggtatcctgt actacatcac gcgcatcatc
     1201 ctcatttttt atattatgca ggatgaaacg gtaaatgccg tcagcatatt gatccacaca
     1261 ggtattgtac tccaatgctg tcactaatag tttcgtgttt aaggataaga cgcactcctg
     1321 ccggtaaagt tacagggatt tttataatta tgagttaagg attataaatt gtgaattgat
     1381 ggcagttcaa aaaatcattt ccaagtaacc atctccaccc ttttttatta cttttgaatt
     1441 atgaataagc tatttactgt atttattttt cttttatgta acaatgctat gaatgcacaa
     1501 aaaacgttaa acggcattta ccgtttacat ggtattcagg atatggcagc agcctttcag
     1561 tttcttcccg acagcacatt tgagtttttt tattcctatg gtgctgttga ccgcaaagca
     1621 aagggaactt acacctatga tggtgacact ataaaattaa atagtgataa aattggtggt
     1681 gcagattttg aaattactga acaacgcatc agtggtaaga cctataaagt tgttgttcgt
     1741 gatgaaaacc aatatctgac acaaaaggta agggcaattg ttactttcgg caatctgaca
     1801 aaagaatatg aggccaatga agatggcgtg ataaacattg atacaaaaaa ttgtgaaaaa
     1861 atatttttag ttcatgagct atatcccgat gtagcaagta caataaaaga tgagttaaat
     1921 gccaacacct actttgaagt cagactcaaa ccctcattag aagatgttag ttttaaaggt
     1981 attaacctct ttaaaaacgg tgaaaacttt aaatgtctgc caaactattt tttgcctttt
     2041 tccaacatac tatttataaa ggaagattaa tttcttggtc aatttattca gcacttctta
     2101 actttttctt ttgattatgg tttaattttt taagcaactt tgtctgcttc aattttataa
     2161 atctataaga aatgagaatt ttacttgtaa tcctaacagc aactgcaggt gttttagcac
     2221 taacgtcatg caaacaaaca ccaaagcagg aaaacaccac acctatagcc aagacaacac
     2281 ctgaagtaga gatcgtggca gatcgctttg ccgatattca aatgctgcga tatactattc
     2341 cgggctttga agaactcagc ttacagcaaa aacaattagc ctactatctt tatatggcag
     2401 gaatgtcggg gcgtgatatt ttttatgatc agaaaaaccg ctacaatctt cagataagaa
     2461 agactataga aacaattctt gaaacttatc agggtgataa gaacgaagac aattacaaaa
     2521 agtttgttgt ttatgctaaa cgatttttct tcagcaatgg cattcatcat cattactcca
     2581 cagataaaat gatacctgaa tttgatccgg catttttaga tactttaatt tcaaaaagtg
     2641 atttcaataa aatgcccaac gaaggaaaaa atattgaaga gtatattgcc tttttaaaac
     2701 caattttgtt tgatccaaaa attgatgcta aaggagtttc gcttgctgct gatgctgata
     2761 atgttaaagc ctcatcaagt aacctttacg aaggggttac tcaaaaggaa gttgagcagt
     2821 tttataaaag taaaatacaa cccggtgtaa aagaacaacc ttcatggggg ttaaactcaa
     2881 aagttgttaa aataaatgga aaggtagaag agcaggtttg gaaatcgggt ggtatgtatg
     2941 gtgctgcaat tgacaaaatt atttactggc tcgaaaaagc agttaccgtt gcagaaaacg
     3001 atgcgcaaaa agctacgctt gaagcattag ttaagtttta caaaaccgga aacctgaaag
     3061 actacgatga ctactgcgtg ctgtgggtga aagatactgc aagtcgcatt gatatagcca
     3121 atggatttat tgaagtttat cttgatccaa tgcataagaa aggagcttac gaatcggtac
     3181 ttagtcttaa agatttagaa gctacaaaac gaattgctgc aattggaaaa gaggcgcaat
     3241 ggtttgaaga ccattcaccc attttaccgg aacataaaaa gaaaagtgta aaaggtattt
     3301 ccgcaaaagt tattaccgtt attaatgaat gtggtgatgc cgcaccatca actccaattg
     3361 gaataaatct tcctaatcag gaatggattc gtgaaaaagt tggctctaag tctgtttcat
     3421 taggaaatat tgttgcttct tacaattatg cccgagccaa aagcccgatg attgatgagt
     3481 ttggtgaaaa tccacaagtg atagaacgtg taaaaaaata tggtgcttta gccggtgacc
     3541 ttcatacaga tatgcatgaa gttatcggac atgcttcagg aaaaataaat gatggtgttg
     3601 ctaatactga tgtaacctta aaaaattatg caagcacatt ggaagaagca cgtgcagatt
     3661 tggttggact atattacatc acagataaaa aattgattga tattggtgta atgccaacta
     3721 ttgaagtagg aaaagccgaa tatgactcat acattttaaa cggactgatg acacagctta
     3781 acagaataaa tcccggtgat aatctggaag aatcgcatat gcgcaaccgt caacttgttg
     3841 catcatgggt ctatgagaaa ggtaaagagg ctaatgttat tgaacgtatc acacgcaatg
     3901 gcaaaactta tttcaaaata aatgattatg ataaactgag aaccttgttc ggtgagttat
     3961 taaaagaaat acaacggatc aaatctgaag gtgattatac ttcaggaaaa agtttagtag
     4021 aaaattttgg tgtaaaagta gatcaggatt tgttgaaaga agttcacgag agatataaag
     4081 cattaaacat tgcaccttat atgggtttta ttcagcctaa gttggttcct gttatgcaag
     4141 gcgacagtat tactgatgtg aaggttgagt acaacaacga ttttattcct caaatgcttg
     4201 agtatggaaa agagttttct tttctgccaa taaaaaatta agtattgctg ttttgttgct
     4261 cagaacattg ttcaattaaa aagcgaagtg tatctacacc atcggcataa tcacttgccg
     4321 atggttgttg tcccattcca aagtttattt cttttccggt aatcacatct gccgaaatga
     4381 tgcattgaat tttatcagct tcagctgtta gcaattgatg caatgaactt tgattgttat
     4441 aggtttcata atgcaatact gataccgggg aggcaatatt ttgctgttct aataagtgta
     4501 cgaagttatt agtaagaaaa ggtatgtttt ccattaagta tatagcccgg taataatcgt
     4561 agttattgat gtatttatta tgcaataaca attcattacc atatggctca attgcatcaa
     4621 agaatttttc aaaattgtaa ttctcaggca ccattagctt ggatacatta cggcaaccta
     4681 aaccaaaata tgaaaagata tcctcgccca aagcaaacaa ctgctccttg gtttcattac
     4741 cattcaagac tgctacactg tttctgtttt ttctgataac atgtggcacc ttactgaaat
     4801 aataatggaa ataacgagcc gtattgtctg aacctgttgc aataacggca tcgggttttt
     4861 gtaatttgtc cacaaaagaa atccgattac ccagctctgc attctcgctt ttaataatac
     4921 ctgttatcca ttccatcaat atgctgtcag atgatgaagg tttaatctga attttgtttc
     4981 ctgaaaccaa taccgtcatc aaatcatgaa aacccacagc aggaatattt ccggccatca
     5041 ccacaccaat atttttagga agatgctcag aaatgctgta acagctaatc cactgctgca
     5101 acgaatccgg ttgcaacata taggcaatgg catttaatgc gttaatctga ttgtgtgctg
     5161 taaaccatgg atttttggct tttgcagtag ccactatatc tttaagttgc ccattgggat
     5221 actgctcaca atgttcttta attctttgcc ctacacggat aaaggtattt atgatatttt
     5281 ctgtctgtga cataaaactt tttttaagaa tttcagtaaa acaagtgcaa actaactttt
     5341 tagtataaaa acttgtttat aaaataaatt tatatttgca ccaacacttc aaataaaaac
     5401 acaaaattat ggctattata attactgacg aatgtatcaa ctgcggagcc tgcgaacccg
     5461 agtgccccaa caacgctatt tacgaaggcg gagcagaatg gcgattctct gatggaacct
     5521 ctgtaaaagg tatgtttaac aaaatggatg gtactgccat tgatgccgat gcagcacagg
     5581 caccggttaa tttagatcat tactatattg ttcctgataa atgtacggaa tgtgttggtt
     5641 ttcatgacga accacagtgt gcttcagttt gcccggttga ctgctgtgta gatgatgaaa
     5701 atgtcagaga atcgaaagat gagttacttg caaaaaaatc acgcatgcac caataatatt
     5761 tgattgaaat ttgcctaaaa tctgctgcac gcttttaaat atgtagcaga ttttttcgtt
     5821 ttatacatca tgttcagaac agctattact ttttttatct tgttttttgc cacaggcaac
     5881 acttttgccc aactacagtt gtttgcagac agtgttatta aaggcagtag cgattcagta
     5941 aggcagcaat atcagctaaa gctgacccat gccatgaaca attttcttgc taccgagcat
     6001 tcattcagca catcgttcga taccatacct tcaatatcaa cagttatatc atccgatgga
     6061 ctcataagat tttatcagtg ggtacaaccg aatctggaaa ccaaaaccta cagcatttat
     6121 ggaatgatgc aggtaaaaga taaaaaaact aagacaataa aaacttatac acttacagat
     6181 ggtgctatgg acaaatacac agcatcagac aaaattttaa atactgataa ttattttgcc
     6241 gcaatttatt acagcatcat ttcattaaaa gaaaacaaga aaacaatcta caccctactc
     6301 ggctggcgtg gtgttgacaa cagaactact gtcaaaacaa ttgacgttct gcattttcaa
     6361 aaaaacaaac ctgtttttgg taaaaaaatt tttaaagctc ctgccaacat gcttcccgtt
     6421 atgagtgccg aaaaatgtac gcgtgtaata tttcaataca atgcacaggc agtcatgagt
     6481 ttaaagtatt attcaaaagg aaaaaaaatt gtttttgatc atttaagtcc gcctaaagct
     6541 actttaaaag gtgctgaaga aacctatgga cctgatttta cttatgatgc ctttatctgg
     6601 aaaaaaggca aatggcaagg caaaagtgat gtggacatca gaagtagcca taacacagac
     6661 ggccagaaaa ttacccctgt aaaagattct gagctgcgga agtagtttat tggctttaaa
     6721 gagctaattt tgccttccga aatatcagat aaaaaacaat ttaatatatc aactatgcgt
     6781 aaacataatt ttggtgccgg ccctggtgta ttacctgaag ctgcaataaa gaaaacacaa
     6841 gaatcacttt ttgattttaa caacagcgga atgggtgtga tggaaatttc gcaccgtagc
     6901 aaagattttg atgctgttct tcaaaaagca aagtcgctgg taaaagaatt acttcacgtg
     6961 cccgacacgc atgaagtact tttcttacaa ggaggtgcaa gtacacagtt tgccatggta
     7021 ccaatgaatt ttcttaaaaa gaaagccgca tatattgatc agggtgcatg gagtaacaaa
     7081 gctattaaag aagctaaact ctttggcgaa gttgaagtga ttgcatcaag caaagacaaa
     7141 aattactctt atcagccaaa aggatatact attcctgccg actgtgatta tctgcatatc
     7201 tgtagcaatg aaactattca cggcactcag gtaaaaaatt tccctgcaag ctctattcct
     7261 gtaatctgtg atatgagcag tgatattttt tcacgtcctg ttgatgtttc aaagtttgac
     7321 ttaatttatg caggtgcaca aaaaaatatg ggcccttcgg gagtaacact tgccatcatt
     7381 aaaaaagaca ttgttggtaa atcagaaaga gtgatgccaa ccatgctcaa ctataaaacg
     7441 catctcgaaa acgattcact ttataacaca cctcctgtaa ttgctattct tgtttgcatg
     7501 catgtattgg agtggctaaa atcaattggt ggtgtagaag ccatgcaaaa aataaatcag
     7561 cagaagtttg atatgtttta taatgaagtt gacagcaatc cattatttac aggaacagca
     7621 gcagccgaag accgctctcc aatgaatgct tgttttcttt taaaagatgc atctatggat
     7681 gaagctttct tagccaaagc aaaagaagcc ggcattgtag gtattaaagg tcatcgtagt
     7741 gtaggtggtt ttcgtgccag catgtataac gcacttcctg ttgaaagtgt aaaagtattg
     7801 gtacaagtga tgaaagattt tgcaaaagct aacggttaaa taaaaaaata tttagttatg
     7861 attaaaatat tagccaatga tggcattgat gctgcaggga aaaaaatatt tgatgctgca
     7921 ggatttacag tttcaacaga caaagttcct caggaaaaac ttgctgaagc attgaagaat
     7981 tttgatgcta tattagttag aagtgctaca acagttcgca ctgatgttat agatgcatgt
     8041 ccaaatctta aagtaatagg tcgtgccggt gtaggtatgg ataatattga tgtaaagtat
     8101 gcccgtgata aaggacttgc tgtaattaat actccggcag catcatcaca atcggttgcg
     8161 gaattggttt ttgcacattt gtttaccgga gtgagaatgc tgtatgatgc taaccgcaac
     8221 atgcctgcct gctctactaa agaaacattt gacaaactga aaaaatctta ctcagcagga
     8281 atagaattaa aaggaaaaac aattggcatt attggtgccg gacaaattgg caagtgcgta
     8341 gctgccatag ccgttggttt gggaatgaat gtcttgtttc acaggctgaa caatgaagat
     8401 gtggttgtag atatggactt tcatcctgca ttaggacaga aaaaaattac acttactttt
     8461 aaatgtgtgt cgtttacaaa gcttcttatt gagagtgatt ttataaccat gcatgtaccc
     8521 tttccgaaag gaagcgcacc ggttatcggc aaaaaagaac ttgacctgat gaaaaatggt
     8581 gtaggcattg ttaacactgc gcgtggtggt gtaatttcag aagtagattt aattgaagca
     8641 ctcaactcag gaaaagtagc atttgcaggg attgatgttt ttgaaggcga accattaatc
     8701 aaccaaacac tttgcacaca tcctaaagta actttgtcac ctcacattgg tggttcaaca
     8761 aaagaagcac aggagcgaat aggcattgaa cttgcagaaa aagtagttga atattttact
     8821 aaaaaataaa atcagaataa aaatttacca aaacgtatgg cagtattaaa accattcaaa
     8881 ggaataagac caaaaaaaga attagtaaag cggattgctt gccgccccta tgatgtactt
     8941 gacgaaaaag aagcacgtgc agaaaccaaa ggcgacaaaa tttctttcta tcatgtcata
     9001 aaacctgaaa ttgattttcc ggatgacttc gatcattatg cccctgaaat ttataaaaaa
     9061 ggtaaagaaa atttcgaagc catgtttaaa gacggtgtgt tttttcagga cagtaaagac
     9121 tgctactaca tatatgcaca aagcatggat ggtcgtaaac aatacggtat tgttggctgt
     9181 gctgcagttg atgattacgt taatgatgtt atcaaaaaac atgagcttac tcgtcctgat
     9241 aaagaagaag accgcaaaaa ccacgtgcgc gtttcaggat tgaattatga gcctgtattt
     9301 tttgcatatc cacaagtgag aaaattagat gaactggttg agcatattac cgctgctgct
     9361 cctgaatatg attttgtaac tgacgatcac atcacacatc agttttggat agttgacaac
     9421 gattcagaca tcaacatcat caccgaagaa tttgctaaac taaaaaacac ctacgttgct
     9481 gatggacatc atcgcacagc agcagctgct ttggttggca aagaaagacg cgaaaaaaat
     9541 ccttcacaca caggtaaaga agaatacaac ttttttctgg ctgtacattt ccccgacaat
     9601 cagttaaaaa taatggatta caacagggta atcaaagacc tgaacggaat gaatgctgta
     9661 cagtttattg acaaactgaa agaaaatttt attgttgaag aaaaaggaag tgatgcgtat
     9721 cgtcctgctg ctttgcatga gttcagcatg tatctggaag gaaaatggta taaattaaat
     9781 gccaagccgg gcactttcaa tgacaaagat cctattggtg ttcttgatgt aactattcta
     9841 tcagataaag ttctggcacc catgttaaac atcacagacc tgcgtaaaga caagagaatt
     9901 gattttgtag gcggacttcg tggtcttggt gaattaaagc gaagagttga taacggagaa
     9961 atgaaggtag cctttgctct ttatcctgtt acaatgaagc aacttattga tattgcagac
    10021 aataatctca tcatgccacc aaaagtgact tggtttgagc ctaaacttcg tagtggcctc
    10081 gtattgcatt cattagacta attttcttac agtcaagtat ttaacaaccg gctgttgact
    10141 gtctgcccaa cagacactgc cggttgattc atatttttta cctttgacct caatttgaga
    10201 gaaccaatag aagcttatga tgcatgacga tgactatgaa ggatttgatg atcaaagcga
    10261 aaatttaggt aacagcgttc agcgttttga agaaatgctg cgcaatcatg aaacctactt
    10321 ttttgatgcc gattcgctga taaaaattac tgacttttat ttagagaagt ttgactatga
    10381 caaggcaatg gaagtcataa aatatggtca gaatcttcat cccgacaata ctgcttttaa
    10441 agcaaaagaa gcctacatct cccaccttca gggtaattcc gaagaagcgt tggaaatgat
    10501 agatcagcta cttaccgtga atccaaaaaa cgaagacctt tatcttataa aaggtcttat
    10561 atataatgca ttgggcatgt ttcaggaagg acgtaaaact ttcaaagact tactgtcatt
    10621 cacagagttt aaagaagatg tttacttcca tgttgcacaa acctacttgc agacattcga
    10681 ttatcacgga gccatacatt atttcaaaaa ctgccttttc gaaaacaaaa aaaacgaagt
    10741 tgctgctgac gaactaagcg aatgttttga ccgtgcagga agagcatcag aaggcattac
    10801 ctattttcag gcattaattg atcaggatcc ttacaatgca ttgttttggt ttcaccttgg
    10861 acaaatgtat cagtgttgtg ctgagtttga aaaagctatc aacgcttacg actattgtat
    10921 tctgattgac gaaaacatgg acgatgctta catacaaagc ggacagtgtc acatgttggc
    10981 aggcaactac gaaaaagcca ttcaagcttt caaacaaagt tttgagtata acacacctga
    11041 ggcatttgtt tatgatagta ttgcacagtg ttattttgaa ttaggcaacc atgaagaatc
    11101 acgaagctac tttaagaagg ctgtaaaaag caatccatca tttcacagtt catggtatgg
    11161 cattggaatg accttgtcgg aagaagaacg ttggtacgaa gctattcatt atctaaaaaa
    11221 agcaattgaa cttaatggcg atattagtga ttattggttt ttgcttggag attgcgaacg
    11281 cgaattaggt aacttagaag aagctgatga agcttacaga aaagtgattg aaatggatcc
    11341 tgatttttta gatatctggt atgagtatcc cgaatttctg atggaacaaa atcgtaccga
    11401 tgaagcactt caatatgctt atcttgcagt aaaatatttt ccaaatgaca ttgcagtcca
    11461 tctgcgatac atcattttac aatatagaaa tggaaacata aaaacagctt acagtttgct
    11521 gtctgctgcc gccaacaacg aagaaaatat cagggaaatt attgaagaaa tttatcctga
    11581 acttctgaac gactctaaca ttcagaaaat tttagaacaa aacaattagt ggcaatgaat
    11641 tttgaattga gtcaaatgcc ttcacgaagt acaaaacctc gtgagaatgg cttaactatg
    11701 gtgatggata aaggtcttag ccttcgtgaa acagaagatt ttatttcttc ctcgggcgaa
    11761 tttaccgata tagtaaaatt aggcttcggc agtagcctca taactccgaa tctggataaa
    11821 aaaatcgcac tttataaaga agccggaata ccggtttatt ttggcgggac tttattagaa
    11881 gcttttctta tcagagatga gttcgacact taccttaaag tattatcaaa atacaaaatg
    11941 gaatatgctg aaatatcaga tggctccatt gtaattccac ataaaataaa atgcgattac
    12001 attaaaaaac ttgctaaaca cgtaaccgta atttcagaag taggctcaaa agaagaaggg
    12061 attattattc gtcctaatgt atggattcaa atgatggaac gcgaattaga agccggtgtt
    12121 tggaaagtaa ttgcagaagc tcgcgaaagt ggtacagtag gtatttatcg tcctaacgga
    12181 aaagcacacg taatgcttat caataaaata atttccagag tgcctgctga taaaattatt
    12241 tgggaaacac cacaaaaatc gcagcaggtc tattttatta agcaatttgg caccaatgtg
    12301 aatttaggca acattgcacc caatgaagtt attgctcttg aatcgctgcg tttaggatta
    12361 agaagtgata cttttttcca ttttcttgat aaagaaacta tcaataaatt taccaattaa
    12421 acttattgct tttcaggcct aatcgtgctt tgcaaagttg tacttttgca gccgcccaaa
    12481 agggtaagat tttatatttc catttccttt acattcattg actgcaagca ccaaaataaa
    12541 tttaaaaaaa ctgctgatat ttattctatt ggcagggttc gtagctaaga ttgctatcgg
    12601 atttcatctt aaagaatcct ttttgaatcg tggtaattca aaaaccaatc ttaatgcgct
    12661 tgcctgtaac ctgtacatta acggtgaatt cagtcttgtt cagggaattc cttccgttga
    12721 ctatgagcct ttgtacccgg cattaatgag tatctgttat gcaattacag gatataattg
    12781 gttaggcctg acaatccttc aggctacctt gcatctcatc acttcactcc ttttatttaa
    12841 aattggcagc cgactttttc atcctattgc aggatttttt gctgcttgtt atcatgcact
    12901 ttatccttat ctattcactt attcgttttc tatttacgac acaacgcttt ttgttttttt
    12961 attagtatta acaatagatt tattacttac agaaaaactt tcatcaaaaa gacttgtact
    13021 tgccggaata ataattggtc tgacattttt aacccgggca acaattataa cttttcttcc
    13081 ggggcttttc ctctatgtat tctatcgcta cttcaaatcg cagaatatta cagcagcatt
    13141 tatcagctgc tcattaattg tgataggatg ttttctggca atgtcgccct ggttggtcag
    13201 aaattattca ctaacaggaa aattattaat cagcacacat ggatcatttg gcatgtggca
    13261 aggcaacaat ccattatcat ttgaatatct taaagctaat aaatcattag acgaaatcta
    13321 tcgtttaaac ccaccggtac caatatatgc acagtatcca cttacagcac gcacacctga
    13381 cgaggcagta aaagttgctg cagcctatca acaagaagcc ataaattgga taaaaaataa
    13441 tttccctgaa tttgtttcta tgggttggct taaagcagaa aaactttgga gttggaaccg
    13501 taaccctgat agtaaaagtg tgaaattcgg aagtaatgaa ggacgaaaat ctgtttatct
    13561 gatttcatac ctaccattgt tattgctctt tccatttgga ttgatattgt tatttaaaaa
    13621 acatcctgct gaggcattta cgctgttact aattctactt gctttcactg gtgcacatgc
    13681 aatagccatt ggattcacca gagcacgcat acctattgat ttttttctga tgctatgcag
    13741 tggcataacc ataacctggt tgctgcaacg attttttcca tctctatatc ttaaatttaa
    13801 atcatcatga gtgacaaaaa atatacggtt atttttctgg gtaaattgcc tccaccctac
    13861 attggcccct ctgttgcctg cgaaattatt cttaactcaa aactcaaaga cgaatttaat
    13921 ctgattcatc ttgacctttc agataaacgt gacatcaata cattagcaaa actcgatttc
    13981 acaaattttt atctggcatt taaacaatat tttcagctat tttggtattg tattcgctat
    14041 cgcccaaatc ttgtttatgt tccggcaggg caaaccaacg ttggctacac acgcgatgca
    14101 ggatatattc tgatcgcaaa gctttttggt ataaaagtac ttacacatct tcgtggcgga
    14161 tattttctga actggtataa tgaaagtgga aaaacgatga aatcttttat tcgttttgta
    14221 cataaaaaag ttaatggaca gattgtgctt ggcaacaacc tgcgtaaact atttaactgg
    14281 attgtacctg atgaaaaaat atttgttatt ccaaatggtg gtaattttcc ggtaaactat
    14341 aatgagcgaa acaacaataa ggttaaagta ctttttttag gaaactatgt gcgtaccaaa
    14401 ggagtcttag aagcattgta tgcaacacca ttagtgtatg aaaaaaataa aaatgtagaa
    14461 tttgtgtttg ccggtgaatg gcgcgaagaa gatgtgaaaa cagattttga aaactttctt
    14521 caacaacatc ctgaacttcc tgtagtaaat cacggacctg ttaaaggtga taaaaagttc
    14581 agtatcctga acgaagccga tatttttgtt tttcctacat actatcgtaa tgaaggtcat
    14641 ccgtgggtaa ctgttgaagc tatggctgcc ggactgccag taatttctac agaccatgca
    14701 gcaatctccg aaagtgtaca tgacggcaaa aacggttatc ttgtagaaaa gaaaaatatt
    14761 cagcaattag ccgaaaaaat aattattttg gcaaacgata aagaactgag aaataaaatg
    14821 ggtgcagaaa gcagacgtat ctacttagaa aatctgacag aagaaaaaat ggttgaacga
    14881 atgggccatg cattcagaag tgtaatagag aattaaagtt tattgaattg aaaaaagtat
    14941 tagtaacagg cggagcaggt tttattggca gcaatttatg cgatgccctt ttagctaatg
    15001 accaatatga agtgcattgc cttgatgtgt ttgatgattt ttataatcct gccttaaaaa
    15061 gaaacaatat tttagaagca ctaaagagta aaaacttttt tttgcacgaa ggtgatattc
    15121 gtaacactac ctttctagaa aaattatttc aacaaccatt tgatattatt gttcatcttg
    15181 ctgcacgtgc cggagtgaga ccgagtattg aaaaaccctt actttatgag gacgtgaata
    15241 tcaaaggaac tataaacctg ctggagaagg caaaagaaaa aaatatcaaa cagtttgttt
    15301 ttgcaggcag cagcagtgtt tatggaatca acccaaacac accatggagc gaaaatgatt
    15361 ttgttttgaa acctatctcg ccttatgcat caacaaaagt tgcatgcgaa cttaccgggc
    15421 atagctactc gcatttatat ggcatcaggt ttatatctct acgtttcttt acagtgtttg
    15481 gtcctcgcca acgacctgac ttagctattc ataaattttt taaattgata cgtcaggaaa
    15541 agtcaattcc attttttggt aatggtgaaa caagccgtga ttatacctat gtcagtgaca
    15601 ttgtgaaagg cataattgca gctatgcatt acgataaaag caattacgaa atcattaatc
    15661 ttggtaataa cagaaccata aatctgaata tgctgattgc agctatagaa aaagaagcgg
    15721 gtaaaaaagc aatacttgaa aaactacccg atcagcccgg tgatgtaccg catacttttg
    15781 ccgacataag caaagcaaaa gaattactta actatagtcc tgctacaact ttagaagaag
    15841 gattaacagc attcaggaag tggttcgatc aaaattattc tgcataaaca atttaaactg
    15901 caaaaaatgg aacagttaac acaaaatctg aaagatggaa aaatgcagtt gcttgaagtg
    15961 ccttttcctg tgctttcatc aggacaggtt ttagtacgca atcattattc actgattagt
    16021 gcaggcacag aaggcaaaac cgtaaaagat gcacgtctga gttatatcgg taaggcaaga
    16081 gcaagaaaag aagaagtaaa aaaagtgatt gatgcagcaa aaacattcgg tttattaaac
    16141 acctataaaa tggtgatgaa taaattagat gcaccatcat cattaggcta tagttcagca
    16201 ggcgaaatta ttgccgttgc gcctgatgtt acagaattca gagttggtga tttggttgct
    16261 tgtggcggca acagtgctgt acatgcagaa gtgattgctg tgccggtaaa tctttgtgtt
    16321 aaactaaacg ataaaattgc ccttcaacat gcatgcttta caactttagg ctctattgcc
    16381 ttgcagggca tcagacaagc tgacctccgc ttaggtgaaa attgtgtggt tatcgggctt
    16441 ggtctggttg gacaacttac cgttcaaatg ctgaaagcag gtggtataaa aacaatagga
    16501 attgatattg accaacgtat ggttgacctt gctttgcata ctgatattga cgctgcattt
    16561 acccgaaaca gtgaagacct cgaaagttct atcaataaca ttactaacgg ctacggcact
    16621 gacgcagtaa taattactgc cggcaccgac tcactcgacc ccgttgatct tgcaggtgcc
    16681 ttatgtcgca gaaaaggaaa agtagttata gttggtgcag tacctacagg gtttaaacga
    16741 cccaactatt ttaaaaaaga attagacctg cgaatgagtt gttcttatgg tccggggcgt
    16801 tatgattcag aatatgaaga aggaggaata gactatcctt acggctacgt acgctggaca
    16861 gaaaacagaa acatggctgc ttttgttgac ttaattgcaa ccggaaaaat caatctgact
    16921 aatctgctga cacatacatt tgattttaaa gaagcacctg cagcatacga ccttatcatg
    16981 aacaaaaccg agccttttgt tggcatggtt ttgaaatatg acactcacaa acctttacaa
    17041 caaaaagtaa atctctccaa cccttcttat actaagagtg atgtggttgc cggtatgatt
    17101 ggcgcaggtt ctttcggaca aaatttttta ttgcctgcat taaagaatac gcctgcaaaa
    17161 ctcagtggca ttgttactgc acgaccgaat aatgcgcgaa acattgcaga taaaaatcaa
    17221 tttgcttttg catcgggtaa tgctcaggat attttcagca atggtgatat taatacagtt
    17281 ttcattgcta cacgccacga aacacatgca ggatatgtta tgcaggcatt acagtcaggg
    17341 aaaaatgtat ttgttgaaaa acctctttgc ttatcagaag aagaattggt gcagatacaa
    17401 catgaattta ccaagcataa ctgcagggta atggttggct tcaacagaag gtttgctcca
    17461 atgattcaaa ccattaaaaa aactttttct tccggaaatc caattgccat aaattacaga
    17521 atcaatgcag gcgtaatccc tgctgatcat tgggtgcatg acttaaaagt tggcggtgga
    17581 cgaattgttg gtgaagcctg tcactttatt gatttatgta tgtttcttgc cggctcaaaa
    17641 cttacaacta taagtgcagt tgcattgaat gatgcacaaa aactaaacga taccgtttgc
    17701 tttaacctac aatttgaaaa tggcagtacc gcttcggtaa gttatttcag caatggcaat
    17761 aaaaatatca gcaaagaata tcttgaagta tttggttctg gcattgtcgc agttcttgat
    17821 gattttaaaa cactgactgt attcggtaaa tctgaaaaga aatataccgg taatcaggat
    17881 aaagggcatc agaatgaggt taaagcattt gtagaaagta ttattcaggg caaacccgca
    17941 cctatacctt ttgaagacac tgttttgagc acgctggcaa cattccgtgc attacaaagc
    18001 attgcctcca atggcgaaag aataattctt taattcaatg gatgcatcac gaacaattga
    18061 agcggcaaca tcccaactgc gaaaatatat tgaggcgcat cgctacaagg gatacgaccc
    18121 ttacgatgca ttaaaatctc cgctttttaa attacccttg ctgaataaaa acaagttgat
    18181 tcgctttggt gcacaacaat tagttaaacg atcgccactc aatttacgaa cactgctttt
    18241 tgttcccaaa ggactgaatc ctgttactct tggcctttgt attcaggctt atgcgcaact
    18301 gattcatgtt aatcagcaag aaaaaaatgt acttaaccaa aatatttcta accttattaa
    18361 tgaacttgaa aaacttatcc ctaaaggatt tcatggtgca tgttggggat atgattttga
    18421 ctgggaagca cgttatgcaa acattcctgc atatcaacca acagttgttg caacaggaat
    18481 aattaccaat gctcttttta tagcatggca acaaacagga aatcaaaaag cattagacct
    18541 atgtaaaagt gctgccaatt ttgtgttaca agacttgaat cgtactaccg acagcgatgg
    18601 cagcttttgt ttttcctatt caccattcga taagcaggtg gtatttaatg caagcatgaa
    18661 aggctctcgt ttgttgtctc aggtttattc agtaacaaaa gatgaaacac taaaaaaggc
    18721 agctgcagat tctgttgcct ttgtcatgaa acatcaacgt gcagatggtg catggattta
    18781 ctcaacaagt gatgcaggaa catggattga caactatcac accggttatg ttttagattg
    18841 tttgcatgaa tatatcttac actgtgacga caagacgtac aatgaaaacc ttgaagcggg
    18901 aaaaaaattt tacattaata acttttttct tgaaggtcat attccaaaat tttataacaa
    18961 caatgtcttt ccggttgact gtacagctgc agcacaatct cttctgacat taacccgatt
    19021 tgatgaacag accctagcca ttaaagttgc agaatacatg cttgaaaata tgcagcattc
    19081 aaagggttat ttttatttca gaaaatataa aaattataca gaaaagacat catttatgcg
    19141 ttggagtaat gcctggatgt ttgccgggct gaccgctgtt ttacaaagcc aatgtgccca
    19201 taggcaatga ggccttaagt tacatcaatt tgcagcatta ttaaattcat agcttggcta
    19261 tacccttgct aatcactatt tttcacttta ttttttagtt gaatagtatt tattggttga
    19321 gtttgcatat ccccttctca tttgtacatt tgcacacatt gcactaagtc cggaatttat
    19381 tgagtaacaa atgaagttta ccgccaaaca aatagcagac ctcctcaaag gcactgtaga
    19441 gggaaatcac gatgccgaag tgaattcttt gtctaaaatc gaagaaggca aaccgggaac
    19501 tttatccttt ttatcaaacc caaaatacac accatatatt tacgatacca aggcatcggt
    19561 tgttattgta aataaagact ttgtggctga aaacactatt aatgcaactc tcatcagagt
    19621 tgaagatgcc tacaaaagtt ttgcctcact gcttgaaata tacaatcaga tagttaatga
    19681 taaaaaaggt gttgaatcgc cttcttttat tgacccttct gcaacagtcg gtgatcaagg
    19741 ttacattggt gcttttgcat atattggcaa gaatgcaatt atcggtaaaa acgtaaaaat
    19801 ttatccgcat tgttatgttg gtgatcatgt tatcattggt gacaatacga ctttgtttcc
    19861 cggtgttagg gtttattctt tttgtcatat aggttctcat tgcaatatcc atgcaggtgt
    19921 aattattggt gccgatggtt ttggatttgc tcccaacgaa aacaacaatt ataaaaaagt
    19981 tccacagata ggtaatgtcg tgattcacga ccatgtagaa attggtgcag gaacaacaat
    20041 tgatcgtgct acattaggat caacagtcat acacaaaggt gttaagttag ataacttgat
    20101 acagattgca cacaatgttg aaattggaga aaacacagtt attgctgcac aaacaggtgt
    20161 ggcaggttca accaaaatcg gaaaaaattg tatgattggc ggacaggtag gtatcatcgg
    20221 gcacttagct attgctgatg gtgtaaaaat tgctgcacaa tccggtatag gaactagtat
    20281 cacaacacct aacgaaattg tacaaggttc accggcattt aatatcagcg attacaaacg
    20341 cacatacgtt gtgtttaaaa agttgccaca attagaaaga aaaattaacg agttagaaaa
    20401 aaagacaggt agtctaaacc ctgctgaaaa taaatcatga gtgtaaaaca agttacttta
    20461 aaagaagaaa taactgtaga aggtccgggg ttgcacaccg gtgtaaatgc caggctgaca
    20521 ttgaaacctg caccggaaaa tcatggatat aagtttaaaa gaaccgatgt aaacgggcaa
    20581 cctgttattg atgccgatgt ggacaatgtt gtggacacat cacgtggaac aacactaagt
    20641 gcaaacggtg taaaggtaag tactaccgaa catgttttag cagcactggt aggcatggaa
    20701 gtagataatg tgcttattga aattgatggc cccgaagtac ctattcttga tggcagtgcc
    20761 tttccgtttg taaatgccat tcaaaaggcc ggcttacgtg agcaagaagc aacacgcagc
    20821 tattttgtta ttacagaaaa cctcacttat gaagagcctg cccgcaatgt tgaaatgctg
    20881 gctgtaccaa gtcccgacta tagaattacg gtaatggttg attacaactc gcctgtgttg
    20941 ggaacacaac atgcgcaaat gcataaaatt accgagttca aagatgaaat ttcaaaatgc
    21001 agaacgtttg ttttcttgca tgaacttgaa atgctgctta agcacgactt aattaaaggt
    21061 ggcgacctga ataatgcaat tgtaattgtt gaccgcgaaa taaaacaaga taagttagac
    21121 gaattggcta ccttattcaa gaaaccaaaa gttgagatta agcataaagg tattttaaat
    21181 aatacagaac tgcattttca gaatgagcct gcacgacata aactgttaga tattgttggt
    21241 gactttgcat tgcttggtgt tcatctgaaa ggacatattc ttgcagcgcg cccgggtcat
    21301 gcagccaacg ttgcttttgc aaaacagttt aaagaaatta tcaaaaaaga taaaaaccgt
    21361 ttaaaggcac caatcattga ccccaataaa cctgcactat tcgatatcaa cagaattgaa
    21421 tctttcctgc cacatcgttt tccgtttttg atgattgaca aaatcactga gttgaccgaa
    21481 acaggtgttg taggtgttaa aaatgtaacg gctaacgaat ggtttttcca gggtcatttt
    21541 ccgggtaatc ctgtaatgcc cggtgtattg cttattgaag ctatggcgca aacaggtgga
    21601 attcttgttc ttaatactgt tcctgatccg gggaattatt ggacttactt tttaaaaata
    21661 gaatcggcac gatttaagaa tatggttaaa ccgggtgata ctgttgtctt taagctcgat
    21721 ttgatttcgc ctatcagacg tggactttgc cacatgggtg ggcaagcatt tgtaggcgac
    21781 aaagttgtta tggaagcaga aatgatggct caaattgtaa aaaaatcatg atttcannnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagc cactttctta tataggcata
    21961 aacaatatcg gtctcagacg cagaggtttt actaacgaag atattaatca cattcaggat
    22021 atctatcgca tattgtttgt tcagaatagc aacattaaaa aagcacttac agaaattgaa
    22081 aaagaaattc ctgaaagtac actgcgaagt cagattgttg aattcattca taactcgcca
    22141 aaaggtttga tgcgtggttt tactccatca gacaacgatg atgaataatt gaacaattaa
    22201 taatcacatc gtgtttactg tcaacttaac tgaagccgga aaaaaattca aacagcactg
    22261 gattttcaga aaatgttctg cttctttttc ttcaggtgat gttatcggca ttacaggcaa
    22321 aaacggctca ggcaaatcaa cattacttca ggttatttca ggctatgttt caccaaatga
    22381 gggaaaaatt gagtggaatg agcatgataa aaaaatagaa gctgacgctg tttatcgtta
    22441 tctttcaatt gcctcacctt acttgatgct cattgaagat tttactttag aggaaatgat
    22501 aggttattat ttccggttta aaaaccttag accatcattt aatatcactt ctgtcattac
    22561 tgaatcagga cttgaagcac atagaaataa attgttgagt gaattttctt caggaatgaa
    22621 acagcgaacc aagttactgc ttgccattgc atcagatacg ccacttttgc ttttagatga
    22681 accctgcacc aacctcgaca agcaaaacat tacctggtat caacaatttt taaaaacaca
    22741 ttcagaaggc aggttgattt ttatctgttc aaaccatatt gatgacgaat tatttttgtg
    22801 caataagtgg ctttccgttg aagattttaa attctgaacc acaaaacagc cattgtagca
    22861 tttattcttt caaccgtttt taggtgtggt cttttatttg ataaattatt tttacctctg
    22921 aaaaattagt tcagcacaag aaaaggacaa tttaaaacta aatacttttc tgaatttctt
    22981 acatcttaaa cagaaattca ttttttatcc cattccggca aagtgcagat ataaattgcc
    23041 tgcaaattct tatgactatg atgaacaacg atcccattat tacctacgac cacacgcagg
    23101 aagaaagcaa ctttcgttcg cgctatttta aaaaatcttt atttggcaag aaaaacacca
    23161 tgctgttgtt ctttgctgaa gaggttttaa tactcgggtt tttggcgtac ttagtttatc
    23221 tggtgtttgt ataagatctg gttgttcttc ctaaaagaac tcctgttgtt tgtggttact
    23281 caaacactat cttcccatta taacttttct gcttattact cagcacaata ttataaactc
    23341 cttttgcctc ctgctccaaa ttcaatttta tattaccatc attcatctct aatgtttttt
    23401 gctgaatgag tttgccggtg ttgtcgtata tgcttagcgt taggtttttc tcatttacaa
    23461 aatcatcagg aacagttatg ttgcatttgc ctgcatttgg attagcataa ataagcattt
    23521 ggttatttgc cattctgccc tctccgccac ctatgcctgt tatggcatct atctttgcta
    23581 aataaatatc ctgccccaaa agcgctgtca tgcttgtgct gccaatgttt attgtattat
    23641 caaacgctcc tgcacaataa gcagagcctg aattatcaac tgcaacacta gaacctgatg
    23701 cttgtccaaa atgccttact cctaagcaat caccgttatt attaaatctt gaaataaaca
    23761 tatcataggt attgcttgta ttaatattga atgttccgaa atttgcattc ccagaaaata
    23821 atccagctaa ataaaaattt ccatctgcat cagaaaaaat attattagga ctcttgtaat
    23881 ttccggtaac tgataactgt gtattccaaa taaaatttcc tacggcatca agtttagcaa
    23941 taaaaatatc tgaactatta gcttgaatcg tgttgccgcc aagatttatt gagtcttgaa
    24001 accctccggt aatatataaa ttgtatgaat tatctattgt aaggtcttgt atgtaatcta
    24061 atcctgcatt tccataagtt tttatccact ttacattccc attactatct gctcgtgcta
    24121 taaaaccatc ataattacct tgtgtgatta atgtagcagt gtctaactga aatggagttg
    24181 agcgatataa accatataat attaaatctg ctcctataaa atccagaaag acaccattgg
    24241 tattacctgt aacaggtgca gaaaatttta tttctgcaga aaggcaatta ccggaagaat
    24301 catattgcac aatgccacca ccgggaggaa taaaaaaact gccaaaatgt gaagaatctg
    24361 ccgtttgagt cagcaaataa attttcccat tagcattaac atttatatac ggaagtcttt
    24421 cttctcctat acctcccatt gctcttgccc aaagaaaatt accactggta tccatacgag
    24481 ttaggaaatt gtctgtttga gtacctaatg atgttagcgt tattccatta ccaaaatcta
    24541 cagtgttttt aaaattgccc gttacataaa tgcaattgca tatcgaatca aaaactcctc
    24601 ttgcatactc atggttatta ggctgcatgt aatttcctcc taaggttttc gcccaaacaa
    24661 gattccctga agcatcaaac ttagatataa aaatatcatt gtttccattg ctgtaaaggg
    24721 tgtcacccgg taaatataaa gtgcctccat atgagccaac aagataaata tttatcccat
    24781 ctgttattac attggcttct tctccgtaaa attgatatgc acttccgata tgtttagccc
    24841 attgccagtt ttgagtatgc cctattagcg gcattattac cgttattaaa actataagat
    24901 ttattatcct gttcctcatt cgtagattag taactcgcag attacttact caaacacaat
    24961 tttaccactg taacttttct ttttgttggc aagcgttacg ttatacactc cttttgcttc
    25021 ctgctccaaa ttcaatttta tattaccgtc attcatctct aatgtttttt gctgaatgag
    25081 tttgccggtg ttatcataaa tagataaaac caaatttttc tcattcacaa aatcatcagg
    25141 cacggttatg ttgcatttgc ctgcattggg gttggcataa ataagcagtt ggttatttgc
    25201 cattctgcct tctccacctc caatgccggt tatggcatct atctttgcca aataaatatc
    25261 ctgccccaaa agcgctgtca tgcttgtgct gccaatgttt attg
//
LOCUS       DAOQNP010000041        25074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_663_length_25074_cov_8.946720, whole genome shotgun sequence.
ACCESSION   DAOQNP010000041 DAOQNP010000000
VERSION     DAOQNP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 25074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25074
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_663_length_25074_cov_8.946720"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PKY09_07120"
     CDS             <1..811
                     /locus_tag="PKY09_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNB12666.1"
                     /translation="HIHVNDNLLYDEIKSYLRSIAPEQEEIVKLFKNKQPIFEHFGID
                     KQIKSDFGKTVALQNSAYLVIEHTEAMHVIDVNSGGRNRGDSDSQEKNAFETNCMAAK
                     EIARQLRLRDMGGIIVVDFIDMINLEHKRQLHEVLKEEMKKDPARHTVLPPSKFGLIQ
                     MTRERVRPEMKISTTEKCPSCNGTGEIKASILLIDEIENNVRYFAHEQNEKKIILHVH
                     PYIEAYLKKGFISIKWKWYLKHKIKVSIEAVSSFAVTQFAFYNTKGEEIKI"
     gene            912..2657
                     /locus_tag="PKY09_07125"
     CDS             912..2657
                     /locus_tag="PKY09_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNB12667.1"
                     /translation="MRKYFILLVVIFCMSNTISFAGNESTGTDKRFDSFKERFIKDLW
                     KHNPMWATEIGYHAYDSILEVNNEQYRNGQVAFSKSYLDSLKTFDKATLSSLNRMDFG
                     LIENYLNSNIFYITEMKGWQWNPSSYNATGNVAFMLSENYEPLNKRLVNIGKKLSQFK
                     SYYEAAKKNIANPTNEHLKLAIEQNAGGIAALETDLSDSLKQATLKAGVKLSITQSLT
                     DAKKAVEDYIAFLKDFKNDNPRNFRLGKDLYAKKFEYEIQSQYTYQQIYDSAMQRKSY
                     LHHEMYKISDKLWPKYFGTKTKPADSLVMIKMMIDTISFNHVKPDEFQSAIEKQLPEL
                     VKFINEKNLIYIDPSKPLKVRREPAYMAGVAGASISSPGPYDKGGNTYYNVGSLSGWS
                     AERAESYLREYNHYILQILNIHEAIPGHYTQLVYSNNAPSIIKAVFGNGAMIEGWAVY
                     GELMMLENGYGNNEPEMWLMYYKWNLRTVCNTILDISVHTKEMTESQALNLLENEAFQ
                     QKAEAQGKWRRVSVTNVQLTSYYTGFKEIYDLREELKKKMGSSFNLKTFHEKFLSYGS
                     APVKMIRQEMLSEIK"
     gene            2659..4689
                     /locus_tag="PKY09_07130"
     CDS             2659..4689
                     /locus_tag="PKY09_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB12668.1"
                     /translation="MIRFERFLSLIKRQFYIYLGGLFLLLSIRLFFLFRLFSIADIRL
                     NIVEILHAILVGFRFDTMVLMYGMALPVLLSILSLIIGTAKSNGMVMRFNRIYAIIML
                     MLFVIVGIVDVYYYNYFQSHINVLAFGLFDDDTTAVLKSVWTDYPIISVLITWIIIFF
                     LIRLFVNYVFSDKVKSVVIKKAWQGVACSLFVLAMFFLGMRGSIGTFPLQIDDSTVCN
                     NSTVNLIPINGVFAIKEAFLMRSKQMSIEGNITFLKKLGYGSETKAVEDYYGQAPEKD
                     LGKSYFTITANKPSGFKYPNVVFFLMESMSNNNLFYHSTTLNLYGALEKHLTADYFLR
                     NFLPSGNGTINSLEGIMVNTPVTSLAQSEHNSVSYNSSVALPFSNAGYETTFITGGKF
                     GWRNINEFIPHQYFQHVESKDNVLKEIAGSTECEWGVYDQYLFDYVFKKLEKATKPQM
                     IFVLTTTNHTPFHLPETYKPYPVKLTDEIKSKLLTDENLAVKNLTNLQYSNDCLGKFM
                     DKVKQSNFSQNTIVAATGDHNNLMLFDFNESQMYYQRSVPLYMYVPETIQPHVTINKW
                     GSHKDIFPTLINLALPQSTYFNAGDNLFDTLQSRNNIFATDWMSMEAMNEFGAVKYGE
                     SVKYFDWDKNKMLKETSAPSADLQKLLQRARAHYAAMAYFIRNETLKTKQGK"
     gene            complement(4695..4937)
                     /locus_tag="PKY09_07135"
     CDS             complement(4695..4937)
                     /locus_tag="PKY09_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12669.1"
                     /translation="MEDFIGFIGVFILLVAYFLHLFRVIKPDKIFYLLLNFIGASLAC
                     LASVLIHYMPFIILEAAWALVSLISIFKLLLSKPLH"
     gene            5015..6154
                     /locus_tag="PKY09_07140"
     CDS             5015..6154
                     /locus_tag="PKY09_07140"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNB12670.1"
                     /translation="MKFATKALHAGVEPDPTTGAIMTPIYQTSTYVQASPGQHKGYAY
                     ARGKNPTRSQLEKNIAALENAKYGLCFSSGMGATDAVMKLLKPGDEVISGDDLYGGTY
                     RMFTKVFANYGIKFHFINLTDANNISKYINSNTKLIWVETPTNPTMQIVDIKACSAIA
                     KKHNCILAVDNTFASPYLQNPIDLGADIVMHSVTKYLGGHSDVVMGALCMSDDSLYER
                     LAFIHNSCGATPGPMDSFLVLRGIKTLHLRMERHCFNGKQIAAFLRAHPKIEKVYWPG
                     FEDHPNHNIAKAQMRDFGGMISFDLKSHSQEEAFKLASSVKVFSLAESLGGVESLVNH
                     PASMTHASVPAAERVRVGVTDSLLRLSVGVEDIDDLIADLKQAIG"
     gene            6239..8332
                     /locus_tag="PKY09_07145"
     CDS             6239..8332
                     /locus_tag="PKY09_07145"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNB12671.1"
                     /translation="MLKKSTNFLLLMMAMITTITVNAQNTHWCATDEVTNAALQNSAA
                     ARAAQNQLEAFTQQYVQNFQPSYFRSTGTAVYVIPVVFHIIHNWGPENISDAQVYDAI
                     RILNRDYNGQRTDTANIIPDFKALNADVQIEFRLAHKDPNGNCTNGIDRQQSLLTYDA
                     GDQSKLNPWPYNKYLNIWTISYFGSSHASAAAYAYKPGTAPPGKDGIIALHNYVGSIG
                     TGSATGQYTLTHEIGHWLNLNHTWGGTNQPGLACGDDNVTDTPVTKGFTSCPSPSNAD
                     VCNPGIIENYQNYMDYSYCYYMFTLGQKARMWAALNSGAGLRNQVHTATNLANTGTDG
                     GTYICAPTASFSPVQKVICEGGIVSFNDASLNTDTVGITYLWTFNGGTPSTSTDKNPD
                     SIVYNTAGIYDVTLTVTNSAGFDTYTGIESVVVRPQQVSNFVPYSEGFENVLFPSVDW
                     IVNNENGNAWELNSVAAHSGFNSVRINNFSGNVAGTKDVFITPGFNLTNTTQTQFTYW
                     LAFAMKSNGSSDVLKVYASNNCGQLWNLRTTKTGSQLATVANAVMSDFIPNASQWRQE
                     TVSLTSGQYSTKPNVSLKFEYTHDLGNNIYIDDINLTGTVGISDYAVSASDVTIVPNP
                     ASEKSNVNFELLQPEKVLITVTDMLGKEVHRIAENRLDAGEYQYEINTNWAKGVYTVR
                     IAAGSQIISKKLVVE"
     gene            8396..8809
                     /locus_tag="PKY09_07150"
     CDS             8396..8809
                     /locus_tag="PKY09_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNB12672.1"
                     /translation="MSENEFSIEMNYVVYAKPEAVFNALTNPDIMKEWIEGSFVFELK
                     KGGNVELFDGWVKGEVLSFINNESLSYSWKPNTWDKKTKASLVKIELKPDAAGTRVFI
                     HHSLFPSNDDTDKHRHGWINFVLDPLNDYFIGLMK"
     gene            complement(8974..9345)
                     /locus_tag="PKY09_07155"
     CDS             complement(8974..9345)
                     /locus_tag="PKY09_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HNB12673.1"
                     /translation="MATKKAKKAPAKKAAPKKKAAPKKKAAAPKKKAAAKAKTKRTPN
                     AAFMKAMTIGEALHPVVGSKPLPRTEVVKKLWEYIKKNKLQDPKEKRNINADDNLKKV
                     FGGKKTVSMFEMTKLVSKHLS"
     gene            9786..11216
                     /locus_tag="PKY09_07160"
     CDS             9786..11216
                     /locus_tag="PKY09_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12674.1"
                     /translation="MKKTIFLMFTCVVCLSTVRGQDMWGIANSNYAGNFAIDINPSSM
                     AVSPIKYELNILSGNMTFYNNYLYLPKGKMSVGKFFKADYFNYDWQDQYTGKDKNAFL
                     QTGIKGPSFFLRSGQTAFGLHTGLKAMGSVRNVDFEMAKLFYENSTYTPLQHTELEAG
                     NNKLAALLYGEIGASFAQQIEAGPGNRIAIGATFNYLLGFDGFYAHNDHMQYRFDDES
                     MLHVSDLKAEYGFAVPQDGESDYFKVRGKGFSTTWGFQYIHGLNPEAYNVYASRLKFK
                     KYKYKVGMSLVDAGSILFAKDTRTVKFDNNNIDWSGFDTTGVESISQLGELIRSKVFD
                     DSLSGYVATKYRAKLPTTISFQFDYCLTHFLYLNTSIIQPVTSKSTAIRRPAQLSFTP
                     RLETWRYEISFPLTWYEYDQLRLGTCLRLGQVVIGSDYLTSMLGLSKFNGFDFFVGIK
                     HSSYAMDKLNKRKKEKAKDTYTGGDI"
     gene            11296..12294
                     /locus_tag="PKY09_07165"
     CDS             11296..12294
                     /locus_tag="PKY09_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNB12675.1"
                     /translation="MNIKQTELFLKKVKGMHTLVIGDVMTDAYLWGRVDRISPEAPVP
                     VVAVQKRESRPGGAANVAVNLHSLGAVPFICSVVGKDNAGAEFKEMIAKSGMPVDGIL
                     SSSKRTTTIKTRVIGNNYQLMRVDAEVAEPLSQTDSDILIKKTVALIRKKKPAVIIFE
                     DYDKGVLNKTVIEAIVAEAQKHDIPTTVDPKKNNFFHYRNVTLFKPNLKELREGLKTD
                     LEKPTATALTKLAYDFAAKQKIKSLLITLSEHGVYYYSKNEKGIIPAHIRNISDVSGA
                     GDTVISVASLAIAAGFSCQEMAAISNLAGGLVCEKVGVVPIDGNQLLNEVKRLSGK"
     gene            12296..13015
                     /gene="ubiE"
                     /locus_tag="PKY09_07170"
     CDS             12296..13015
                     /gene="ubiE"
                     /locus_tag="PKY09_07170"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNB12676.1"
                     /translation="MEVTPYNTGESKKEQVRHMFDAVAHRYDFLNHFLSLGIDKIWRR
                     KTINEIGKLKPRKILDIATGTGDLAIAAASLSPEKIIGVDISEKMLEAGRVKVEARRL
                     NNILSLQQGDSEQLQFDNQTFDAVMVAFGVRNFQNLEKGLKEMHRVLNSNGKVFILEF
                     SKPTLFPVKQFYGIYSRYLLPLFGKLISGDASAYTYLPESVKHFPSGNDFALVLKQYG
                     FTNIEIKPMTFGIVTLYIAAA"
     gene            13012..13722
                     /locus_tag="PKY09_07175"
     CDS             13012..13722
                     /locus_tag="PKY09_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNB12677.1"
                     /translation="MKQKFIVFSFLFLLFSADSLFAQKQKILNLPKYDRQRFHFGFIL
                     GINKTDFVIDRVASFSLIDSLYTVESKGTSGFNLQIVTNMRMGEHFDLRFLPGLAFAS
                     RNLIYSFEKGRPLSNVVTKKVESTFVELPLDVKFKSARLNNFRAYVLGGVKYSIDMVS
                     QAKVKAKDKEYVKLQRNDYGYEIGVGCDFYLPMFKFSPEIKMYHGLRNLLVKDDLVYS
                     KSLDGLFSKMFTLSLTFE"
     gene            13738..15294
                     /locus_tag="PKY09_07180"
     CDS             13738..15294
                     /locus_tag="PKY09_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNB12678.1"
                     /translation="MQTFELVVLPEEAASESVVQHIVAKKFGNNRLFRITKRSIDARS
                     KIVKIRLQLQWIDEQNQKSLPENRMWQNVANSQPVIIAGCGPAGLFAAIRLIECGYKP
                     IIIERGKDLRNRRRDLAAINKQRQINPESNYCFGEGGAGTYSDGKLYTRSNKRGNIAS
                     VLNTFIEHGAAADIAVDAHPHIGTNKLPAIISAMRNRIVESGGEVLFNTKLIKIDFDL
                     SGVNGVEVQQGLSQFHIKSKALILATGHSARDVFYMLNNQKVMIEAKPIAIGVRAEHQ
                     QQVIDAVQYHCSKRSPFLPPASYALKHQHDGRGIYSFCMCPGGIIAPAATGIGEVVVN
                     GWSPSRRNNVFANSGIVVSITEDDVKPFVKAKELRFLELQKSIEEKAYICGGSNLAAP
                     AQRMVDFVNQKVSSNLPACSYIPGINSVNLKEIFPESVTHSLSAAFVAFGKKMKGYYT
                     NEAVLVGTESRTSSPVRIPRDVETAMHVHVHGLFPCGEGAGYAGGIVSAAMDGEFCAE
                     KCAAYLKNNS"
     gene            15327..16412
                     /locus_tag="PKY09_07185"
     CDS             15327..16412
                     /locus_tag="PKY09_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNB12679.1"
                     /translation="MEINQPPYYIRITIKLLLGFLIFYILHVAQDVLIPLTVAVLLTF
                     LLMPVSNKLIKWKFPPTIAILCSILLALVVVAGLFYFFYSQIVSFAEDLPLLQAKLLE
                     KVEFLQHFVNETFRITKEQQNVLVKSKLQENIADSGLLLLDLFSSTGTFIANFALVTV
                     YVFFLTLYKKKFKQFIEAIVTNDNHNSYFEIVKKVAGVSQNYLKGIFLDVLILSILNS
                     TGFMLLGIEHAILFGVLASILNIIPYIGVLIGSIFPVLMALLTKDEIGYAIGAAGVCV
                     TVQFIDNNFITPYVVGSSVSINPLTATIALIISASVWGLAGMVLCLPLTGMLKVVCDH
                     VEGLKPIGFLIGEEVDYRKNKFKLGRK"
     gene            16492..17145
                     /gene="scpB"
                     /locus_tag="PKY09_07190"
     CDS             16492..17145
                     /gene="scpB"
                     /locus_tag="PKY09_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNB12680.1"
                     /translation="MENLKQYIEALIFTSEQSITANEIAAALKASFGWELSNENIRQS
                     IAELINKYAADDYSFELVEIAGGFRFLSKKEYYGVVNTMLQINSKKRLTTAALETLAI
                     VAYKQPVSKSEIENIRGVNCDYSIQKLLEKDLITIQGKGDGPGKPILYGISRTFMDHF
                     GLKSAKDLPKLKDVILDENEIGTPADMQYAESDNAEDISITIGTQEDNTKKSDDVAE"
     gene            17176..18012
                     /locus_tag="PKY09_07195"
     CDS             17176..18012
                     /locus_tag="PKY09_07195"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNB12681.1"
                     /translation="MKISILGLQFDLAFKNVYSMLSIALICLMQPSISEGQEKLRKVE
                     NQAFTYGEVLEYRVHYGIIDAGEAKLAVMPDKKTIGPREVYHVVGTGQTKGAFDWFFK
                     VRDRYESFIDAQAIIPWLFVRRVYEGGYKINQDVSFNQVKNLATSKKATIATPQNVQD
                     LISAFYYARTIDFSNAQPGEMFAVDAYLDDAIIPIKFKFVGKENISTKFGTFRCLKFH
                     PQLLEGRVFKNKEDMTLWISDDENKIPIRAEAKILVGSVKMDIKGFSGLSNENLAIIK
                     NK"
     gene            complement(18015..19085)
                     /gene="pdxA"
                     /locus_tag="PKY09_07200"
     CDS             complement(18015..19085)
                     /gene="pdxA"
                     /locus_tag="PKY09_07200"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNB12682.1"
                     /translation="MSTTTENEIKPKIGITIGDLNGVGIEVIIKTFSDNRMLSSCTPV
                     IYGSSKVISYHRKSLHQQEFNFNTIRTAEEASNKKANVINCWEEDVKIELGKPSAIAG
                     TYAFKSLEAASKDVLGKKIDAIVTAPIDKSTIHSDAFPFNGHTEYFAKLCGNAEHVML
                     MVSDKLRVGLITGHVALKDVPAQLTKEKIISKTKLVHQSLRSDFGLQKPRIAILALNP
                     HAGDNGLMGKEEQDIITPAISELHNAGIGAFGPYPADGFFGSSRFKSFDAIMAIYHDQ
                     GLVPFKALAFSTGVNYTAGLPIIRTSPDHGTAYDLAGKNQASEESFREAVYLACDIAK
                     QRQITNEITANPLKFSRMSADR"
     gene            complement(19088..20512)
                     /locus_tag="PKY09_07205"
     CDS             complement(19088..20512)
                     /locus_tag="PKY09_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNB12683.1"
                     /translation="MIKNKLADNNSDYSLVILTTSASKFDASVFSADEIKYVKDELKN
                     KKSLVAVNQYNRMVYVCLLENKSKEQSLYMESCRKAGHQMTALLNKTNVKSVLIEARG
                     LRQEALCFTEGLALANYQFRKYKTSGNNNSLEVIALHSNKLIDKDLLWLNTMVDATCK
                     ARDLVNEPVNYLTATQLSNEMVKLGKQAGIKVTVLNKKEIEKTGMGGLLAVNKGSQDP
                     PTFTIMEYKPAGHKNKKPFVLVGKGVVYDTGGLSLKPTANSMDYMKCDMGGAAAVGCA
                     IYALAKAKIPLHIVALIPATDNRPGENAYVPGDVIKMYSGKTVEVLNTDAEGRMILAD
                     ALHHAKKYDPALVMEFSTLTGSASSALSKYACAFLTTADQNQNTALKKSAANVYERVV
                     EFPMWDEYGELIKSDVADIKNVGGPAAGMITAAKFLEHFIDYPYMHFDIAGTAFVHAE
                     DSYRGKGGTGFGVRLISEFLKNKI"
     gene            20668..21813
                     /gene="mqnC"
                     /locus_tag="PKY09_07210"
     CDS             20668..21813
                     /gene="mqnC"
                     /locus_tag="PKY09_07210"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNB12684.1"
                     /translation="MNVENLLSRALDFEFLNVEEGMHLFQHAPLTDLMFVANELRKKQ
                     KPENMTETGEELVTWMIDRNVNTTNVCIANCKFCNFFRVPGHKEAYITSIEEYKTKIE
                     EMFRYGGDQLLLQGGHHPELGLSFYTGIFSELKKLYPQVKLHALGPPEVAHITKLEGM
                     THTEVLKALKDSGLDTLPGAGAEILSDRVRRMISKGKCTGREWLDVMRAAHQLNITTS
                     ATMMFGHIETLQERFEHLVMLRQVQSEKPAHAKGFLAFIAWPFQDENTILKRVKGIRN
                     KVTSEEFIRMIALSRIMLPNIINIQASWLTVGKQTAQVALHAGANDFGSIMIEENVVS
                     VAGAPHRFTSQGIQDAIREAGFIPQLRSQQYQFREIPEMMEQQVINY"
     gene            21818..24412
                     /locus_tag="PKY09_07215"
     CDS             21818..24412
                     /locus_tag="PKY09_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12685.1"
                     /translation="MRKISIFLLLLLGLQIEAWATHNRAGEITFNQISLLTYRVQIVT
                     YTKTSSPADRPMLEMNWGDGTQDSLPRISKTTVGIDISRNYYEGVHTFPGPAVYTVFF
                     EDPNRNGGVVNIPGSVNIPFYVESQIVINPVLGYNNSPVLLQPPIDNGAIGKIFIHNA
                     NAYDPDGDSLSYELIKCKGINGLDIPGYFYPAASTSFTLDAVTGDLIWNTPTIQGEFN
                     VAFLIREWRNGVNIGYVERDMQIDIVVTNDNPPVINALNDICVTAGTLISQSVTATDP
                     DAANMITLSATGSPLDTNFTPISPAVFPVVTDTNSVTGIFSWQTECVHVRKAPYQVLF
                     KAQDNVNQINLVDLKRMNITVVAPAPQNLTATPVQNAIHLTWDQSVCFNAEGYRIYRR
                     IGFFGYVPSLCQTGVPAFTGYTYLATVQGLANNTFIDDNNGAGLIPGNDYCYMVIAYF
                     NDGAESYASNEACAKIVQSLPIMTNVSVTATDVAAGTIYVAWSKPKEIDSIQTPGPFE
                     YRLLRGQGFAPSSFTTVSTFSNLDDTTFVDMALNTADNAWTYKVEFHNLTPGNVFKIG
                     ESLAASSVYLTTTPTDNAIQLSWQATVPWTNNATVVYRKNAGGTYAVIDTVSGNTYND
                     TGLANDTLFCYKVETMGGYSSPGFVFPILNFSQEKCTTPKDNVNPCNISSAAVSGSCL
                     NSEVVLTWQKPDSACGDDVLTYELYYAPSKNATPYLIASLDGINQNTYTRNVPDSTSG
                     CYFFMTVDSAGNKSSLSEPYCAETCPVYTLPNVFTPDNDGKNDLFVPFPYRYVDHIEL
                     TIYNRWGNKVFETHDKDILWKGTKDNGSTRLSDGVYYYIGKVYEIFIDGIKPRTIKGA
                     VNLIGGAK"
     gene            24412..25008
                     /locus_tag="PKY09_07220"
     CDS             24412..25008
                     /locus_tag="PKY09_07220"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNB12686.1"
                     /translation="MFTGIIETLGIVKKIENEGSNRHITVASSFVDELKIDQSVAHNG
                     VCLTVVNINHEKGTYKVIAVDETLRKTNLGDFVEGDEVNLERGMLLNTRLDGHIVQGH
                     VDSTAVCTSVTDENGSWKFVFENDGSARNLLVEKGSVTVNGVSLTVVDAGDYSFSVAI
                     IPYTMQHTTFKNLKRGHTVNVEFDIIGKYVARLMEKSV"
BASE COUNT         7913 a         4239 c         5134 g         7788 t
ORIGIN      
        1 ccatattcat gttaatgaca atcttcttta tgatgagatt aagtcatacc tgagaagcat
       61 tgcaccggag caggaagaaa ttgttaagct atttaagaat aagcaaccca tctttgaaca
      121 tttcggaatt gataaacaga taaaatctga ctttggcaaa accgtagctt tgcagaatag
      181 tgcttactta gtgattgagc atactgaggc catgcatgtt attgatgtta acagtggagg
      241 tcgtaacaga ggagatagcg acagtcagga aaaaaatgct tttgaaacca actgcatggc
      301 agcaaaagaa attgccagac aattgaggtt gcgtgatatg gggggtatca ttgtcgttga
      361 ctttattgac atgatcaatc ttgaacataa gcgtcagtta catgaagtgc taaaggaaga
      421 gatgaaaaaa gatcctgcac gtcatactgt tttgccgcca agtaaatttg ggttaattca
      481 gatgacccgt gaacgtgtgc gaccggaaat gaaaatttca actactgaaa aatgtccgtc
      541 atgtaatggc acaggtgaaa ttaaagcaag tattttactt attgatgaaa tagagaacaa
      601 cgttcgctat tttgcgcatg aacaaaacga aaagaaaatt attttgcatg tacatccata
      661 tattgaagca tatttgaaaa aaggatttat ttcaattaaa tggaaatggt atttaaagca
      721 taaaataaaa gttagtatag aagcagtaag ttcttttgcg gttacacagt ttgcttttta
      781 taataccaaa ggcgaagaga taaagatata gtaagattag aatgtttgac attcataaaa
      841 tcttatgaag tatattcagg gatagatttt aaatacacta ccttagcatt ccttaaaaac
      901 cattcataat aatgagaaaa tatttcattt tgttagttgt tattttctgt atgagcaaca
      961 caatttcatt tgcaggtaat gagtcaaccg gcacagacaa acgttttgat tcttttaaag
     1021 aaagatttat aaaagacttg tggaagcata atccaatgtg ggcaactgaa ataggttatc
     1081 atgcatacga ctccattctt gaagttaata atgaacaata tcgtaatggg caagtagctt
     1141 tttcaaaatc atatctcgat tctttaaaaa catttgataa agcgacattg agcagtttaa
     1201 acagaatgga ttttggtctg attgaaaatt atctcaacag caatattttt tacatcacag
     1261 aaatgaaagg ctggcaatgg aatccttcat catacaatgc aacaggcaat gtagcattta
     1321 tgttatccga aaattatgag ccactcaata aacgacttgt aaatatcggc aaaaaacttt
     1381 cacagtttaa atcatattac gaggcagcaa aaaagaacat tgcaaaccca acaaatgaac
     1441 atcttaagtt ggcaatagag cagaatgcag gtggcatagc tgcacttgag actgacctat
     1501 cagactctct aaaacaagcc acactaaagg caggtgttaa attaagcatt acgcaatcac
     1561 tgactgatgc aaaaaaggca gttgaagatt atattgcatt tctgaaagac tttaagaatg
     1621 ataacccaag aaattttagg ttgggaaaag acttgtatgc aaaaaagttt gaatacgaaa
     1681 ttcaaagtca atatacctat caacagattt atgactctgc aatgcagcga aagtcttatc
     1741 tacatcatga aatgtataaa atatcagaca agctatggcc aaaatatttt ggaacaaaaa
     1801 ctaagccagc tgatagttta gtaatgataa aaatgatgat tgatactatt tcttttaatc
     1861 atgtaaagcc ggatgaattt caatcagcaa tagaaaaaca acttcctgaa ttggttaaat
     1921 ttatcaatga aaaaaatctg atttatattg atccttccaa acctctaaaa gtaagacgag
     1981 aaccagcata tatggcaggt gtagccggtg catctatcag ttcacccgga ccttatgata
     2041 aaggaggtaa tacatactat aatgtaggtt cactatcagg ctggtctgct gagcgtgcag
     2101 aaagctattt aagagagtat aatcattaca ttttgcagat tttaaacatc cacgaagcaa
     2161 taccgggaca ttatacacag ttggtctata gcaataatgc accaagtatc attaaagcag
     2221 tatttggcaa tggcgcaatg attgaaggct gggcagttta tggtgagtta atgatgcttg
     2281 aaaatggcta tggtaacaat gagcctgaaa tgtggttgat gtattacaaa tggaatctga
     2341 ggacagtttg taataccatt ttagatattt ccgttcacac caaagaaatg acagaatcac
     2401 aggctttaaa tttgttggag aatgaagcat ttcagcaaaa ggcagaagca caaggtaagt
     2461 ggaggagagt aagtgtaacc aatgttcagc ttacaagtta ttataccggc ttcaaagaaa
     2521 tttatgacct gcgcgaagag ttgaagaaga aaatgggaag tagttttaat ctgaaaactt
     2581 ttcatgaaaa gtttttgagt tatggaagtg caccggttaa aatgattcgt caggaaatgc
     2641 tttcggagat taaataacat gattcgtttc gaacgatttt tatctttaat taaacgacag
     2701 ttttatattt atttaggcgg actctttctg ctactatcaa ttcgcttatt ttttcttttt
     2761 cgtttattca gcattgctga tataaggtta aatattgttg aaatactaca tgcaattctg
     2821 gtaggtttta gattcgatac catggtgctg atgtatggaa tggcactacc tgttttatta
     2881 agtattttat ctctgattat tggtactgca aaaagcaatg gcatggtgat gcgttttaat
     2941 aggatttatg caataatcat gttaatgcta tttgtaatag ttggaattgt agatgtatat
     3001 tattacaatt attttcagtc gcatatcaat gtgttggcgt ttggtttgtt cgatgatgat
     3061 actactgcag tattaaaatc tgtttggaca gattacccga ttataagtgt tttaattaca
     3121 tggataatta ttttttttct gatcagatta tttgtaaatt atgtttttag tgataaggta
     3181 aaatctgttg taattaaaaa agcatggcaa ggtgttgctt gcagtttgtt tgtattggcc
     3241 atgttttttc ttggaatgcg tggttcaatc ggaacattcc cattgcagat tgatgactct
     3301 acagtttgta ataacagtac agtaaatctg attccgatta atggtgtgtt tgcaataaaa
     3361 gaagcatttc tgatgcgaag caagcagatg agtattgaag gcaatattac ttttttaaaa
     3421 aaactgggtt atggtagtga aacaaaagct gttgaagatt attatggtca ggcacctgaa
     3481 aaagatttgg gtaaatcata ctttacaata acagccaata agccttccgg gtttaagtat
     3541 ccgaatgtgg ttttttttct gatggaaagc atgagcaaca acaatttgtt ttatcattca
     3601 acaacactta atctttatgg tgcattggaa aagcatctta ctgccgacta ttttttaaga
     3661 aactttttac cctcaggcaa tggtaccatc aatagtttag aaggcataat ggtcaacaca
     3721 cctgtaactt cattggcaca gtcagaacat aattcggttt catacaacag ctctgtggca
     3781 cttcctttca gtaatgccgg atatgaaaca acttttatca caggtggtaa gtttggatgg
     3841 cgaaacatca atgagtttat tccacatcaa tattttcaac atgtagaaag taaagataat
     3901 gtactgaaag aaattgcagg atcaacagaa tgtgagtggg gtgtttatga tcagtacctt
     3961 tttgattatg tttttaagaa attggaaaag gctacaaagc cacaaatgat ttttgtactc
     4021 accacaacaa atcatacacc ttttcatctg ccggaaacgt ataaaccgta tccggtaaaa
     4081 ctgaccgatg agataaaatc aaaattgctt actgatgaaa atcttgcagt aaaaaatctt
     4141 actaatctgc aatattccaa tgattgctta ggtaaattta tggataaggt taagcaatcc
     4201 aatttcagtc agaacacaat tgttgcagcc accggagatc ataacaattt aatgttattt
     4261 gattttaatg aaagtcagat gtactatcag cggagtgttc cattgtatat gtacgtacct
     4321 gaaacaattc agccgcatgt gacaatcaac aaatggggtt cacataagga tattttcccg
     4381 actttaataa accttgcatt gccacaatca acctacttta atgccggtga taatttgttt
     4441 gacacattac aaagccgcaa taatattttt gccactgact ggatgagtat ggaagcaatg
     4501 aatgaatttg gtgctgtaaa gtatggtgag tctgtgaaat attttgattg ggataaaaat
     4561 aaaatgctga aagaaacttc agcaccatct gccgatttgc agaaattgct tcaacgtgca
     4621 agagcacact atgctgcaat ggcttatttt atcagaaatg aaactttaaa aaccaaacaa
     4681 ggaaaatagt tttattaatg caatggtttc gatagaagaa gtttaaagat tgatataagt
     4741 gaaaccaatg cccatgcagc ctcaagtata ataaatggca tatagtgaat taaaacagaa
     4801 gcaaggcatg ctagagatgc gccaataaaa ttcagaagaa gataaaatat cttatcaggc
     4861 ttgattactc tgaaaagatg aagaaaatat gccactaaaa ggatgaaaac accaataaat
     4921 cctataaaat cttccataaa tgtttaaatt aacgataacc aaaggtatgg caatggttgt
     4981 ataatttatc ttcgcacgtt taaaatttat aacaatgaaa ttcgcaacta aggcacttca
     5041 tgcaggtgtt gagcccgatc caacaaccgg tgccattatg acacctattt accagacatc
     5101 aacctatgta caggcatcac ctggacagca taaaggttat gcctatgcca gaggaaagaa
     5161 tcctacacgt tcgcagttag aaaaaaatat tgctgcattg gaaaatgcaa aatacggatt
     5221 atgttttagc agtggcatgg gagctactga tgcagtgatg aaattattaa aacctggtga
     5281 tgaagtaatt tccggtgatg atttgtatgg tggaacatat cgtatgttta ctaaggtttt
     5341 tgcaaactat ggaattaagt ttcatttcat aaacctgact gatgctaaca atatcagtaa
     5401 gtatatcaac tcaaatacaa aacttatttg ggttgaaaca ccaaccaatc caaccatgca
     5461 gattgtggac attaaagctt gtagtgcaat tgcaaaaaag cacaattgta tattggctgt
     5521 tgacaacaca tttgcatcac cgtatttgca gaacccaata gatttaggtg ctgatattgt
     5581 tatgcattca gttactaaat atttaggtgg gcatagtgat gtggtaatgg gtgcattgtg
     5641 tatgagtgat gattcgttgt atgaaagact tgcttttata cataactcat gtggtgccac
     5701 tccgggtcct atggatagtt ttcttgtatt aagaggtatt aagacattgc acctgcgtat
     5761 ggaacgtcat tgttttaatg gaaaacagat tgctgcattt cttcgtgctc atccaaaaat
     5821 tgaaaaagtt tactggccgg gctttgaaga tcatccaaac cataacattg caaaagcaca
     5881 gatgcgtgat tttggcggaa tgatttcctt tgaccttaaa agtcattcgc aggaagaggc
     5941 atttaaatta gcttcgtctg taaaagtgtt ttcattggcc gaatctttgg gtggagtaga
     6001 gtctttagtt aaccatcctg catcaatgac acatgccagt gttcctgctg ctgaaagagt
     6061 aagagttggc gtaaccgatt cattattgag attaagtgtt ggcgttgaag atatagatga
     6121 tttaattgca gatttgaaac aagcaattgg atagtgccat cacaaaaaaa ttacattgaa
     6181 aatacgttca aaatacatca ttttattact tttgattcat tataaataaa ttaaaacaat
     6241 gctaaaaaaa tctactaatt ttttattgct gatgatggcc atgattacaa ccattacagt
     6301 caatgcacaa aacacacatt ggtgtgctac tgatgaagtt accaatgctg cacttcagaa
     6361 tagtgctgct gcccgtgctg cacaaaatca gttagaggcg tttactcaac agtatgtaca
     6421 aaactttcaa ccaagttatt tcagaagtac cggaacagca gtgtacgtta ttcctgttgt
     6481 gtttcacatc atacataatt ggggtccgga aaatatttca gatgcacagg tatatgatgc
     6541 tatcagaata ttaaaccgtg attataatgg acaaagaaca gatacagcaa acatcatccc
     6601 tgattttaaa gcattgaatg ctgacgttca gattgaattt cgtcttgcac ataaagaccc
     6661 taatggtaat tgcaccaatg gaattgacag acaacaatcg ttgttgactt atgatgccgg
     6721 tgatcaatcg aagctgaatc catggcctta caataaatat ttaaatatct ggaccatttc
     6781 atactttggt tcttcgcatg caagtgcagc agcgtatgcc tacaaacccg gtacggcacc
     6841 tcccggcaaa gatggaatca ttgcattaca caattatgtg ggaagcatag gaacaggtag
     6901 tgcaacaggt caatatacac tcacacatga aattggtcac tggcttaact taaatcatac
     6961 atggggtgga accaatcaac ccggtttagc ctgtggtgac gataatgtaa cagatactcc
     7021 ggttacaaaa ggttttacta gttgtccatc accttcaaat gctgatgtat gtaatccggg
     7081 tataattgag aattatcaaa actacatgga ctacagctat tgctattaca tgtttacact
     7141 gggacagaaa gcacgtatgt gggcagcact taattcaggt gcaggtttac gtaatcaggt
     7201 gcatacagca actaacttag ctaatacagg taccgatggt ggcacgtata tctgtgcacc
     7261 aacagcaagt ttttcacctg tacaaaaagt aatttgcgaa ggtggtattg tgagctttaa
     7321 tgacgcaagt ttaaatacag atacagtggg tataacttat ctgtggactt ttaatggcgg
     7381 aacaccatct acctcaacag ataaaaaccc tgacagtata gtttataaca ctgcaggcat
     7441 ttacgatgta acattaactg ttacgaattc tgcaggtttt gatacttata ctggtattga
     7501 gagtgtagtg gtaagaccgc aacaggtttc taactttgtt ccttattcag aaggttttga
     7561 gaacgtattg ttcccttctg tggattggat tgttaacaat gaaaatggca atgcatggga
     7621 attgaattct gttgccgcac attcaggatt taattctgta cgaataaata atttcagtgg
     7681 aaatgttgcc ggaaccaagg atgtttttat tacaccgggg tttaatttaa caaacaccac
     7741 acaaacacaa tttacctatt ggcttgcctt tgccatgaaa agcaatggca gttctgatgt
     7801 gttgaaagtg tatgcttcca acaattgcgg tcagttgtgg aatttacgta ccacaaaaac
     7861 aggctcgcag ttggctacag tagctaatgc tgttatgtcg gattttattc ctaatgcctc
     7921 acaatggcgt caggaaaccg tgagcctcac ctccggacaa tattcaacaa aacctaatgt
     7981 atcattaaag tttgaataca cgcatgattt gggtaacaac atttatattg atgacatcaa
     8041 cctaacaggt acagtaggca tcagcgatta tgcagtctca gcaagtgatg taacgattgt
     8101 tccaaatcca gcttctgaaa agtcaaacgt taattttgaa ttgcttcaac cggagaaagt
     8161 attgataact gtaacagata tgcttggcaa ggaagttcat cgtattgctg aaaatcgttt
     8221 agatgcagga gaatatcaat atgaaataaa cacaaattgg gcaaagggtg tttatactgt
     8281 tcgtattgct gccggaagtc agattatcag taagaaatta gtagtagaat aaattaaata
     8341 aataataatt aagcctgaag ctgaatttag tttcaggctt ttctttttaa gggatatgag
     8401 tgaaaatgag ttttcaattg aaatgaatta tgtggtgtat gctaaaccgg aagcggtgtt
     8461 taatgcattg acaaatcctg atatcatgaa agaatggatt gagggaagtt ttgtttttga
     8521 attgaaaaag gggggtaatg ttgaattgtt tgatggttgg gtgaaaggcg aagtactttc
     8581 attcatcaac aatgagtcgt tgagctatag ctggaagccg aacacatggg ataaaaagac
     8641 aaaagcttct ttagtgaaaa ttgaactgaa gcctgatgct gccggaacca gagttttcat
     8701 tcatcacagc ttgtttccaa gtaatgatga tacagataaa caccgacatg gttggataaa
     8761 ctttgttctt gatccattga atgattattt tatcgggttg atgaagtgat tcggcatagt
     8821 ttgattatca aaaatcattt gttatttcac actccttaga attcctcctg ccaggggacg
     8881 aaatagtagg cctaacaagt aatgacaata cacagaaaca aaaaagggaa gaccttttga
     8941 gccttccctt tttgttttct ttcttacagg aaattaagaa aggtgtttgc taacgagttt
     9001 agtcatttca aacatagaaa cggttttttt gccaccgaaa acttttttca agttgtcatc
     9061 agcattgatg ttgcgtttct ctttaggatc ctgtaatttg tttttcttaa tgtattccca
     9121 aagtttttta acaacttcag tacgtggaag tggcttgctt cctacaacag gatgcaatgc
     9181 ttcgccaatt gtcatagctt tcatgaatgc agcatttgga gtacgcttgg ttttagcttt
     9241 tgctgctgct ttctttttag gagctgctgc ttttttcttt ggagcagctt tcttttttgg
     9301 ggctgctttt ttagcaggtg cctttttagc ttttttagtt gccattttga aatttgtttt
     9361 ttttaatttg ttgagcaaat gaaatacata attttcgctt tgaagcaacc atacacatga
     9421 atttattaac agctgttttc agagggtaaa caggtaaatt tgcatcatca gtctcacagt
     9481 aatatgagga taatgtttta tcataacaag tgttttctcc tctgaaaagt acatttttac
     9541 caatgagaaa tcaatgcatt ttattgtttt tacctctgaa aataccaagt aattataggt
     9601 tgttgcattg ttaataaccg aagtggttaa ctaccttata gaatttttat aaggtaaaag
     9661 ttgttgattt tctctgattc ttacagataa acttacatta taaatagtag taagattatt
     9721 atattataga ctcaaccatc tttaagtctt tagcgtttag caacacataa caattgtttt
     9781 aactaatgaa aaaaacaata tttcttatgt tcacatgtgt tgtttgcctt tcaacagtaa
     9841 gggggcagga catgtggggg atagccaata gtaattatgc gggaaacttt gccatagata
     9901 taaatccatc ttcaatggct gtttcaccaa taaagtatga gttaaatata ctttcgggga
     9961 atatgacttt ttataataac tacctctatt tgcctaaagg aaaaatgtct gtaggcaagt
    10021 tttttaaggc agactatttt aattacgact ggcaggatca atatacaggc aaggataaaa
    10081 atgcatttct tcaaacaggc attaaaggcc catcgttttt tcttcgttcc ggtcaaacag
    10141 cttttggctt gcatacagga ctaaaagcaa tgggaagtgt aaggaatgtt gactttgaaa
    10201 tggcaaagtt gttttatgag aattctacct acacaccttt gcagcataca gaacttgaag
    10261 ccggtaataa taagttggct gctttattat atggtgaaat aggtgcgagt ttcgcacagc
    10321 agattgaggc aggacccgga aatagaattg caataggtgc tacctttaat tatctgctgg
    10381 gctttgatgg attttatgca cataacgacc acatgcaata tcgttttgac gatgagtcaa
    10441 tgctgcatgt tagcgatctt aaagcagaat atggttttgc tgttccacaa gacggtgaga
    10501 gcgattattt taaagttcgc ggtaaaggat tcagtactac atggggtttt caatatatac
    10561 atggactgaa ccctgaggct tataatgttt atgcaagccg actgaagttt aagaagtata
    10621 agtataaagt aggaatgtcg ttggttgatg ccggcagtat attgtttgca aaagatactc
    10681 gtaccgttaa gttcgacaat aataatatag actggagtgg atttgataca accggagttg
    10741 aaagcatcag tcaattagga gagcttatta ggtctaaagt atttgacgac tccttgtcgg
    10801 gatatgttgc aacgaaatac cgtgcaaaac ttcctactac tatcagtttt cagtttgatt
    10861 actgcctgac ccattttctt tatctgaaca cgtctataat tcaacctgtc accagtaagt
    10921 caacagctat caggcgaccg gcacagttgt cttttacacc aagattagaa acatggcgtt
    10981 atgagatttc ttttcctcta acatggtatg aatatgatca gctaaggctt ggaacatgtc
    11041 ttcgtttagg tcaggtggtt attggttcag attatcttac ttccatgctt ggtttgtcta
    11101 agtttaatgg gtttgacttt tttgttggaa taaaacacag cagttatgca atggataagt
    11161 tgaataagcg taagaaggaa aaagctaagg acacttatac cggaggcgac atttaaaaac
    11221 tactcttgcc ggtcattttt gacgtttaag aaaagtaatt cacattactt acttttgccc
    11281 ttcaattcta ccggtgtgaa tattaagcag actgagctgt ttctgaaaaa ggttaaggga
    11341 atgcatacct tggttatagg tgatgtaatg accgatgctt atctgtgggg tcgtgttgac
    11401 cgtatctcac cggaagcacc tgttcctgta gtggcagttc aaaagcgcga aagccgcccc
    11461 ggtggcgctg ctaacgttgc agtaaatctt cattccctgg gtgcagtacc ttttatatgt
    11521 tctgttgtcg gaaaagataa tgccggagca gagtttaaag aaatgattgc caaaagcgga
    11581 atgcctgttg atggcatact ttcttcttcc aaaagaacca ctactatcaa aacaagagta
    11641 attggaaaca attatcagct catgcgtgta gatgcagaag ttgccgaacc actttcgcaa
    11701 actgacagcg acatactgat aaaaaaaaca gtagcgttaa tcaggaaaaa gaagccagca
    11761 gtaattattt ttgaagatta tgataaagga gttcttaata aaactgtaat cgaagctatt
    11821 gttgcagaag cacagaaaca tgatattcct acaacagttg accccaaaaa gaataatttt
    11881 ttccattatc gtaatgtcac actttttaaa cccaatctta aagagttgcg cgaagggctg
    11941 aagaccgatt tggaaaaacc aacggcaaca gcactaacga agttggcata tgattttgca
    12001 gccaaacaaa agattaagtc attgctcatt accttatcag aacacggagt ctattattat
    12061 tcaaagaatg agaaaggaat tatccctgca catattcgta atatcagtga tgttagtggt
    12121 gcaggtgata cagtaatcag tgttgcatct ttggcaatcg ctgccgggtt ttcatgtcag
    12181 gaaatggctg caatcagcaa tcttgcaggc ggcttggttt gtgaaaaagt aggtgttgtt
    12241 cctattgatg gcaatcagtt gttgaacgag gttaaaagac tttccggaaa ataaaatgga
    12301 agtaacgcct tacaatacag gagaatcaaa aaaagaacag gtgcgccaca tgtttgatgc
    12361 agtagcacac cgatatgatt ttttaaatca ctttctttct ttaggcatag ataaaatctg
    12421 gagacgtaaa acgattaatg agattggtaa actaaagcca aggaaaattc ttgacattgc
    12481 taccggtaca ggtgatttgg cgattgcagc agcaagttta agtccggaga aaataatagg
    12541 agtggatatt tcagaaaaaa tgcttgaagc cggaagagta aaagttgaag caaggagatt
    12601 gaacaatata ttatcattgc aacagggcga cagtgagcaa cttcaatttg acaatcaaac
    12661 ttttgatgca gtgatggtag cttttggtgt tcgtaatttt caaaatctgg aaaagggatt
    12721 aaaggagatg catagggtac taaacagtaa tggaaaggtt tttattctgg aattcagtaa
    12781 accaacactt ttccccgtta aacaatttta tggcatctat tcacgttatc ttttaccact
    12841 tttcggcaaa ttgatttccg gtgatgcatc tgcctatact tatttacctg aatcggtaaa
    12901 acattttcca tcaggaaatg attttgctct tgttttgaaa caatatggtt ttactaatat
    12961 tgaaattaaa ccgatgactt tcggaattgt aactttatac attgcagcag catgaaacag
    13021 aagtttatag ttttttcttt cttatttctg ctgttcagtg ctgatagtct gttcgcacag
    13081 aaacaaaaaa tccttaacct gcctaagtat gacaggcagc gatttcattt tggatttatt
    13141 cttggaatca acaagaccga ttttgtgatt gacagagttg cttcttttag tttaattgat
    13201 tcactttata ctgttgaatc aaagggtaca tcaggtttta atcttcagat agtaaccaac
    13261 atgcgaatgg gagagcattt tgatttacga tttttacccg ggttggcatt tgcttcgcgc
    13321 aatctgattt attcatttga aaagggcaga ccactcagca acgttgtgac caaaaaggta
    13381 gagtcaactt ttgtagagtt gcctttggat gtaaaattca agtcagcacg attgaataat
    13441 ttccgtgcct atgtattagg aggagttaag tacagcattg atatggtgtc gcaggcaaaa
    13501 gtaaaagcta aggataaaga atatgtaaag cttcaacgaa acgattatgg atatgaaatt
    13561 ggagtaggat gcgattttta tcttcccatg tttaagtttt ctccggagat aaagatgtac
    13621 cacggtttac gcaatctttt ggttaaagac gatttggttt attcaaaatc tcttgacgga
    13681 ttgttttcaa aaatgtttac cttatcattg acattcgaat aacagaagtt actttttatg
    13741 caaacatttg aacttgttgt tctgcccgaa gaagcagcca gtgagtcagt agttcaacac
    13801 atagttgcca agaaatttgg taacaacaga ttgttccgta ttacaaagcg tagtattgat
    13861 gcccgatcca agattgtaaa aatcagattg cagttacaat ggatagacga acagaatcaa
    13921 aaatcattac ctgaaaacag aatgtggcag aatgttgcca atagccaacc tgtaataatt
    13981 gcaggatgcg ggcctgcagg gttgtttgca gccatccgcc ttattgaatg tggatataaa
    14041 ccaataatta ttgaacgtgg caaagaccta cgcaatcgca gacgtgatct tgctgcaata
    14101 aataaacaaa gacagattaa tccggagtca aattattgtt ttggtgaagg tggtgcaggc
    14161 acctacagcg atggaaaact ttatacccgc tccaataaga gaggcaacat tgcttctgta
    14221 ttgaacacat ttattgaaca tggcgcagct gctgatattg ctgttgatgc acatcctcat
    14281 attggaacca ataaacttcc tgctataatt tctgcaatgc gaaacaggat tgttgaaagt
    14341 ggaggagaag tgttattcaa tacaaaactt ataaaaattg attttgacct gagtggtgtg
    14401 aatggcgtag aggttcagca aggattatct caatttcata ttaaatctaa agcactgatt
    14461 ttagcaaccg gacattctgc acgagatgtc ttttatatgt tgaacaatca aaaagtaatg
    14521 attgaagcaa agcccattgc tattggtgtt cgtgccgaac atcagcagca ggttattgat
    14581 gctgtgcagt atcattgcag caaacgcagt ccgtttttac caccggcatc gtatgcatta
    14641 aaacaccaac atgatggacg gggaatttat tctttctgca tgtgtcccgg aggaatcata
    14701 gcaccagcgg caacaggcat tggcgaagta gttgttaatg gttggagtcc ttcaagaaga
    14761 aataatgttt ttgccaactc cggaatcgta gtaagtatta cagaggacga tgttaaacct
    14821 tttgtaaaag ctaaagagct gcgcttttta gagttgcaga agagtataga agagaaagca
    14881 tatatctgcg gtggaagtaa tcttgctgca ccagcgcaac gtatggttga tttcgtgaat
    14941 caaaaagtat cttcaaatct acctgcctgc tcctatattc cgggaatcaa ttcagtaaat
    15001 ctgaaagaga tatttcctga atcggttaca cattcacttt ctgctgcatt tgttgcattt
    15061 ggaaaaaaaa tgaaaggcta ctatacaaac gaagcagtgt tggtgggcac agagtcgcgc
    15121 acctcctcac cggtacgtat tccacgtgat gttgaaacag caatgcatgt tcatgttcat
    15181 gggcttttcc cctgtggtga aggcgcaggc tatgccgggg gcattgtgtc tgcggctatg
    15241 gatggagaat tttgtgcaga aaaatgtgca gcatatttga aaaacaatag ttgaaatagt
    15301 tacttttaaa accacattct ttaacaatgg aaatcaatca gccgccttat tatatcagaa
    15361 taacaattaa acttctgttg gggtttttaa ttttttatat cctgcatgta gcacaggatg
    15421 tacttattcc actcacggtg gctgtattat taaccttttt gttgatgcct gtgtcaaaca
    15481 aattaataaa atggaaattt cctcccacta tagcaatctt atgtagtatc ttacttgcat
    15541 tagtagttgt agctgggctt ttttattttt tctattcaca gattgtttca tttgctgaag
    15601 acttgccatt gctgcaggca aaactccttg aaaaggtaga gttcttgcaa cactttgtaa
    15661 acgaaacatt cagaattacc aaagagcaac aaaatgtttt ggtaaagtca aagttgcagg
    15721 aaaatattgc tgacagcgga ttactgctac ttgatctttt ttcttcaacc ggaactttta
    15781 ttgccaactt tgccttagta actgtttatg ttttttttct gacactgtat aaaaagaaat
    15841 ttaagcaatt tattgaagct atagttacta atgataatca taattcatat tttgagattg
    15901 taaaaaaagt agcaggagtt tcacagaatt atttgaaagg tattttctta gatgtactca
    15961 ttttatccat cctcaattca acaggattta tgttattggg cattgaacat gctattttgt
    16021 ttggagtgct tgcatctatt ctcaacatca taccctacat tggtgtattg ataggaagta
    16081 tttttccggt gcttatggca ttactcacta aggatgaaat tggctatgca attggtgctg
    16141 ccggggtttg tgttactgtt cagtttattg acaataattt tattactcct tatgttgttg
    16201 gcagtagtgt cagtatcaat ccgctcacag caacaatagc cttaattatc agtgcctcgg
    16261 tgtggggctt ggcaggaatg gttttatgcc tgccactgac aggaatgtta aaggtagttt
    16321 gcgatcatgt tgagggctta aaacctatag gatttttaat aggcgaggag gtggactata
    16381 ggaaaaataa atttaaactt gggcgaaagt aggcctgttg aaatgaaatg ctaattttgc
    16441 attttatagt gaattgacaa aagccgattc agattaaaat caagagtaaa aatggagaat
    16501 cttaaacagt atattgaagc attaattttt acttcagagc aaagtataac tgcaaatgaa
    16561 attgctgctg cattgaaagc aagtttcgga tgggagcttt ccaatgagaa tatcagacag
    16621 agtattgctg aattaatcaa caaatatgct gctgatgatt attcttttga gttggtagaa
    16681 attgccggag gatttcgttt tctcagtaaa aaagaatatt atggtgtagt aaacaccatg
    16741 ttgcagatta attctaaaaa acgacttaca actgcagcat tggaaacgct tgcaatcgtt
    16801 gcatataaac aaccggtttc aaaatcagag attgaaaaca taagaggtgt aaactgtgat
    16861 tattctattc agaaattgtt ggagaaagat ttaattacca tacaaggaaa aggtgatggt
    16921 ccgggaaaac cgattttata tggcattagc cgcacattca tggatcattt cggtttaaaa
    16981 tctgctaagg atttaccaaa gcttaaagat gtcattcttg atgaaaatga gattggaacg
    17041 cctgccgata tgcaatatgc agagagtgat aatgctgaag atataagtat tactatagga
    17101 acacaggaag acaatacaaa gaagtctgat gacgttgctg aataatcagt ctttaattga
    17161 aattcatttt tagaggtgaa aatttctatc cttggcttgc aatttgattt ggcttttaaa
    17221 aatgtttata gcatgttgtc aattgcactc atttgtttaa tgcaaccaag tatcagtgaa
    17281 ggtcaggaga aactacgtaa ggtagagaat caggctttta catatggtga agtgttggag
    17341 tatcgtgttc attacggaat aatagatgca ggcgaagcaa aacttgctgt gatgcctgat
    17401 aaaaaaacta ttggccctcg tgaagtatat catgttgtag gaacaggtca gactaaaggt
    17461 gccttcgact ggttttttaa ggtgcgagac cgttatgaaa gttttattga tgcacaagca
    17521 attattccct ggttgtttgt cagaagagtt tatgagggtg gatataaaat caatcaggat
    17581 gttagtttca atcaggtaaa gaatttagca acaagcaaaa aggcaaccat agccacaccg
    17641 cagaatgttc aggatttgat ttctgcattt tattatgcac gcactattga cttcagcaat
    17701 gcccaacccg gtgaaatgtt tgctgttgat gcctatttgg atgatgccat tattcctata
    17761 aaattcaagt ttgtggggaa agagaatata agtactaagt ttggtacttt tcgttgcctg
    17821 aagtttcatc cgcaattgct ggaagggcgt gtttttaaaa acaaagaaga catgacactt
    17881 tggattagtg acgatgaaaa taaaatcccc atacgtgcag aagcaaagat attggtaggg
    17941 tctgtaaaaa tggacataaa aggtttttca ggactttcca acgaaaattt agctatcatc
    18001 aaaaacaaat agtcttatcg gtcagccgac atgcgcgaaa atttcagagg attagcagta
    18061 atctcgtttg tgatttgtcg ttgttttgca atgtcgcagg caagatagac agcctcgcgg
    18121 aatgattctt ctgatgcctg attttttccg gccagatcgt aagcagtgcc atggtcgggt
    18181 gaggtgcgga tgattggcag gcctgcagtg tagttgacac cggttgaaaa agccaatgct
    18241 ttaaagggta ccaatccctg atcgtgatag atggccatga tggcatcgaa actcttaaat
    18301 cgtgaagagc caaaaaaacc atcagcaggg tagggtccga atgcaccaat accggcatta
    18361 tgcaattcgc tgatggcagg tgtaatgata tcctgttctt ccttacccat taaaccattg
    18421 tcgcctgcat gtgggttgag tgcaagtatg gcaatgcgtg gcttttgcag tccgaagtcg
    18481 ctgcgaagtg actggtgtac caattttgtt tttgaaataa ttttttcttt ggtaagttgt
    18541 gcaggtacat cttttaatgc tacatgacct gtaatgagtc cgacacgcag tttatcgctg
    18601 accatgagca taacgtgttc ggcattgccg cagagctttg caaaatattc tgtatgtcca
    18661 ttgaaaggaa atgcatcact gtgaatggtg cttttgtcaa taggtgctgt aactatggca
    18721 tcaatttttt taccgagtac atctttactt gctgcttcga gtgatttgaa tgcataggtg
    18781 cctgcaatgg cagaaggttt gcccaattca atttttacat cttcttccca gcagttgatg
    18841 acattggctt ttttattact ggcttcttcg gctgtgcgga tggtattgaa attaaattcc
    18901 tgctgatgca aagattttct gtgatatgaa ataacttttg aagaaccata gatgacaggt
    18961 gtacagctag agagcattct gttgtcggaa aaagttttaa tgatgacttc tattccaaca
    19021 ccattcaggt cgccaatggt aattcctatt ttaggtttta tttcattttc agttgtagtt
    19081 gacataatca gattttgttt tttaaaaatt cagaaattaa ccgcacacca aatcctgttc
    19141 cgcctttacc acggtaagaa tcttcggcat gaacaaatgc agtgcctgca atatcaaagt
    19201 gcatataagg ataatcaata aaatgttcaa gaaattttgc tgctgttatc attcctgctg
    19261 cagggccgcc aacgttttta atatcggcaa catcagattt tatcagctcg ccatactcgt
    19321 cccacattgg aaattctacc acacgttcgt aaacattagc agcgcttttc tttaatgcag
    19381 tgttttgatt ctggtctgcc gttgttaaaa atgcacaggc atatttactc aatgcacttg
    19441 atgcagatcc tgttaaggta gaaaactcca tgaccaaagc aggatcgtat tttttagcat
    19501 gatgcaaggc atctgcaaga atcattctgc cttctgcatc agtatttaaa acttcaacag
    19561 tttttccgct gtacattttt atcacatcac cgggaacata agcattttca ccggggcggt
    19621 tgtccgtagc aggaatcaat gctactatat gtaaagggat ttttgctttt gctaatgcat
    19681 agatggcgca tcctacagct gcagcaccac ccatgtcgca tttcatatag tccatactgt
    19741 tggccgtagg tttcagactt aatccgcctg tatcatacac tacgcctttg ccgactaaga
    19801 caaatggttt tttgttttta tgtcctgccg gtttgtattc cataatggta aaagtgggag
    19861 gatcctgact gcctttgttt acggcaagta aaccacccat gcctgttttt tcaatttctt
    19921 ttttattcag aacggtaact tttataccgg cctgtttgcc cagcttaacc atttcgtttg
    19981 ataactgcgt tgccgtaaga taattgacag gctcatttac cagatcacgg gctttgcagg
    20041 tggcatcaac catagtgttt aaccacaata aatctttatc gattaatttg tttgaatgta
    20101 aggcaattac ttcaaggctg ttgttgttgc ctgaagtttt gtatttccta aactgatagt
    20161 tggctaaggc aagtccttct gtaaaacaca atgcttcctg acgcaatccg cgtgcttcaa
    20221 tcaacacact ttttacattt gttttattca gtaatgcagt catctgatgg cctgccttgc
    20281 ggcatgattc catgtatagc gattgctctt ttgacttatt ttccaacaaa cagacataaa
    20341 ccatgcggtt atattggttt acagcaacca agcttttttt atttttcaac tcatctttta
    20401 catactttat ctcatcggca gaaaatactg aagcatcaaa ttttgaagca gatgtggtga
    20461 ggattactaa tgaataatcg ctgttattgt cggcaagttt gtttttaatc atatctttaa
    20521 agtaatgtta attaaaatga cagaatcaac ttctcatctg ccataaaatt ctataaattt
    20581 acgcattgaa aagaaagacg tttgcggccg tgaaattata aaaacaaatc acaccgtttg
    20641 ctgttgagta aagtgaattc ttaaagtatg aatgtagaaa atttactgag tcgtgcactg
    20701 gattttgaat ttttgaatgt tgaagagggg atgcaccttt ttcagcatgc accattaacc
    20761 gacctgatgt ttgttgccaa tgagttgaga aaaaagcaga aacctgaaaa catgactgaa
    20821 acaggagaag agcttgttac ctggatgatt gaccgcaatg taaatactac caatgtatgt
    20881 attgccaatt gtaagttttg taattttttc agagtaccgg ggcataaaga agcctacatc
    20941 accagcattg aagagtataa gacaaagatt gaagagatgt ttcggtatgg tggcgatcag
    21001 ttgttgttgc aaggaggcca ccatccggag ttaggtttat cattttatac cggcattttc
    21061 agtgagttaa agaagcttta tccacaggtg aagttacatg cacttggccc accggaggtg
    21121 gcacacatca ctaagctgga agggatgacg catactgaag tacttaaagc cttgaaagat
    21181 tcagggcttg ataccttgcc cggtgcagga gcagaaattt taagtgacag agtgcgcaga
    21241 atgatttcaa aaggaaaatg tacagggcgt gaatggcttg acgtgatgcg tgcagcacat
    21301 caactaaaca ttaccacatc tgcaacaatg atgtttggtc atatagaaac attgcaggag
    21361 cgatttgaac atttagtcat gctcagacag gtgcagtctg aaaaacctgc acatgcaaaa
    21421 ggttttcttg cctttattgc atggccattt caggatgaga atacaatttt aaagagagta
    21481 aaaggcattc gtaataaagt tacttccgaa gagtttatta ggatgattgc gttgagcaga
    21541 atcatgttgc caaacattat aaacattcag gcatcgtggc ttactgtagg taagcaaaca
    21601 gcacaagtgg cattgcatgc aggagcaaac gattttggct ctatcatgat tgaagagaat
    21661 gtagtgagtg ttgccggtgc accgcatcgt tttacatcac aaggtattca ggatgctatt
    21721 cgcgaggcag gatttattcc acaattgagg tcacaacaat atcagttcag agaaatacct
    21781 gaaatgatgg aacaacaagt tatcaactac tgattgtatg agaaaaattt caatcttttt
    21841 gttgctgctc ttaggtttgc aaatagaagc atgggcaaca cacaatcgtg caggtgaaat
    21901 aactttcaat cagatatcat tgctgactta cagagtgcaa attgtaacct atactaaaac
    21961 aagcagccct gccgacagac ctatgctcga aatgaattgg ggtgatggca cacaggactc
    22021 tttgccgcgt ataagtaaaa ctactgtagg cattgacatc agcagaaatt attatgaagg
    22081 tgtccacact ttccccggac ctgctgttta tactgttttt tttgaagatc caaaccgcaa
    22141 tggtggtgtg gtaaatattc ccggctcagt aaatattcca ttctatgtag agtcgcagat
    22201 tgttatcaat ccggtgctgg gatataataa ttctcccgta ttgttacagc cgccaattga
    22261 taatggtgct ataggaaaaa tatttattca taatgccaat gcttatgacc ctgatggcga
    22321 tagcttatcg tatgaactga taaaatgtaa gggtattaat gggcttgata taccgggata
    22381 tttttatccg gctgcaagta catcatttac cttagatgct gttacgggag atttaatttg
    22441 gaatacgcca accattcagg gtgagtttaa cgtggctttc ttaattcgtg aatggcgtaa
    22501 tggtgtaaac attggctatg ttgaacgcga catgcagatt gatattgttg taacaaatga
    22561 taatcctcct gtgataaatg cattaaatga catttgtgtc actgcaggaa cgttaatttc
    22621 tcagagtgta acagcaaccg accctgatgc agcgaatatg atcactttat cggcaacggg
    22681 tagtccgtta gacaccaact ttacacccat aagccctgct gtttttcctg ttgtaacaga
    22741 taccaactca gtgacgggaa tatttagctg gcagacagag tgtgtacacg ttcgcaaagc
    22801 accctatcaa gttttgttta aagctcagga taatgtcaat cagataaatc ttgttgacct
    22861 gaagagaatg aacattaccg ttgttgcacc ggcaccacag aatcttactg caacacctgt
    22921 gcaaaatgct attcatctta catgggatca gtcggtgtgt tttaacgcag agggatatcg
    22981 tatttacagg cgaattggat tttttggata tgtgccgtca ctttgtcaga caggagttcc
    23041 ggcttttaca ggatatactt atctggcaac ggttcaggga ttggcgaata acacttttat
    23101 tgacgataat aatggcgcag gattaatacc cggaaacgat tattgctaca tggtgattgc
    23161 ttattttaat gatggagcag agagttatgc atctaatgaa gcctgtgcta aaattgttca
    23221 gtcgctaccc attatgacca atgttagtgt aactgctact gatgttgccg ctggaaccat
    23281 ctatgtggca tggtctaagc ctaaggaaat tgacagcata caaactccgg gaccttttga
    23341 atatcgattg ttacgcggac agggttttgc accatcatcg tttaccactg tttcaacctt
    23401 cagcaatctt gacgacacta cctttgttga tatggctctg aatactgctg acaatgcatg
    23461 gacttacaaa gtagaatttc ataacctgac accgggaaat gtatttaaaa taggagagtc
    23521 tcttgccgct tcaagtgttt atctgactac cacaccaaca gacaatgcca ttcagttgtc
    23581 atggcaggca acagtacctt ggacaaataa tgcaactgtt gtttatagga aaaacgcagg
    23641 tggcacttac gcagttattg atactgtttc aggaaacacc tataacgata ctggcttggc
    23701 aaacgatact ttgttttgct acaaagttga aacaatggga ggttactcat caccgggttt
    23761 tgtctttcca attctgaatt tttcacagga gaaatgtacc acaccaaagg ataacgttaa
    23821 cccatgtaat atcagcagtg cagcagtgtc aggttcatgt ttgaatagtg aagttgtttt
    23881 aacatggcaa aagcctgata gtgcatgtgg tgatgatgtg ctaacttatg aattgtatta
    23941 tgctccttca aagaatgcaa caccttattt gattgcttct ctggatggaa ttaatcaaaa
    24001 tacatacaca cgcaatgttc ctgacagtac aagtggttgt tattttttta tgactgttga
    24061 ttcggcaggt aataaaagca gcctcagcga gccttattgt gcagagacat gccctgttta
    24121 tacattaccc aatgtattta cccctgataa tgatggcaaa aacgatttgt ttgttccatt
    24181 tccttatcgt tatgttgatc acattgaact caccatttac aaccgttggg gaaataaagt
    24241 ttttgaaact catgataagg atattttgtg gaaaggaacc aaagataatg gctcaacaag
    24301 actgagtgac ggtgtgtatt actacattgg aaaagtgtat gaaattttta ttgacggcat
    24361 taaaccacgc accatcaaag gtgctgttaa cctgattgga ggtgctaaat aatgtttaca
    24421 ggaattattg aaactttagg cattgtaaag aaaatagaaa atgaaggcag taaccgtcat
    24481 attactgttg catcttcttt tgttgatgaa ttaaaaatag accagagtgt tgcacataat
    24541 ggcgtttgcc ttactgttgt aaatattaac cacgaaaagg gaacctataa ggttattgct
    24601 gttgatgaaa cattgcgtaa aactaatctt ggcgattttg ttgaaggcga tgaagtaaat
    24661 cttgaaagag gcatgttgtt gaatacacgt ttagatgggc atattgttca gggacatgtt
    24721 gacagcactg ctgtttgcac ttctgtaacc gatgaaaacg gcagttggaa atttgttttt
    24781 gagaatgatg gctcagcacg taatttatta gttgaaaaag gctctgttac tgtcaatggt
    24841 gtaagtctta ccgttgttga tgccggtgac tattcttttt ctgttgccat tattccttac
    24901 accatgcagc ataccacatt taaaaatctt aaacgtggac atacagtaaa tgttgagttt
    24961 gatattatcg gaaagtatgt ggcaaggctg atggaaaaat cagtctgata aatttactcc
    25021 cgtataattt tccggtgtaa ttttcagtaa ttcagctttt atggtatcgc taac
//
LOCUS       DAOQNP010000042        24399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_693_length_24399_cov_8.775592, whole genome shotgun sequence.
ACCESSION   DAOQNP010000042 DAOQNP010000000
VERSION     DAOQNP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24399
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_24399_cov_8.775592"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..73)
                     /locus_tag="PKY09_07225"
     rRNA            complement(<1..73)
                     /locus_tag="PKY09_07225"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            482..1222
                     /locus_tag="PKY09_07230"
     CDS             482..1222
                     /locus_tag="PKY09_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNB12687.1"
                     /translation="MSESSQKHFSFKEFKVHQEQCAMKVGTDGVLLGSWVECNYSVSN
                     VLDIGTGTGLLALMLAQKCSALIDAIDIDSDACNQAKENFYHSKWSDRLSVIHKPIQE
                     FAAISNKKYDLIVSNPPFFINAKKSQQLNRNVARHLDDSFTMDDLITSVAKLLSDSGK
                     FFLIQPVKEGSIFMEESEKRGLYVNNITWVRTKEDKEEKRLLLNLSRNKKKIITDYLS
                     IQTKDGKYTDEYIKLTEEYYTHLPERSF"
     gene            complement(1367..2692)
                     /locus_tag="PKY09_07235"
     CDS             complement(1367..2692)
                     /locus_tag="PKY09_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNB12688.1"
                     /translation="MRKILVLGAGKSSWHLINYLLFNAQEQNWHVTVADASLELAKSR
                     TRNHPSSNAIAFNINDESERKSEINKADIVISLLPPSLHAIAAVECLVAGKNLVTASY
                     VSEQMKSFDADVKNKNLIFLNECGLDPGIDHMSAMQIIHSLQSKGAEIISFKSYTGGL
                     ISPESNNNPWGYKFTWNPKNVILAGQGTALYRQNRSNKFTPYHRLFSQTEKINISGIG
                     QFEGYANRNSNAYIETYNLLNISTLLRGTLRYIGYCSSWNLFVHLGITDDTYKINTSS
                     LTYHDWISSYLPDDGKPLLQKLQTQFHYLNLSSDDWNRITWTGILEKTLIPISNGTPA
                     EVLLNLLESKWKLQHGDLDLVVMQHIFEYKLNSKTHTHISSLSVKGENETATAMSKTV
                     GLPMAIAAKLILNGEIKTKGVTIPVIPEIYEPVLNELSELNIIFKVEQG"
     gene            2882..3358
                     /locus_tag="PKY09_07240"
     CDS             2882..3358
                     /locus_tag="PKY09_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12689.1"
                     /translation="MRVILSCFLFIIIGLKSDEDMKLFARVVENLSTTDIVVLNKLID
                     EVRISNTSISRACHGTLLMKKADFVKRPSEKLSLFKEGRKLLETEIKKDSANVNFRIL
                     RLIIQENCPVFLKYNTNLDDDAVFVQRNFDKLSNKIRDLLITYSKNSKHLKLKESE"
     gene            3351..4259
                     /locus_tag="PKY09_07245"
     CDS             3351..4259
                     /locus_tag="PKY09_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12690.1"
                     /translation="MSKQILVLYYSQTGQLEQIVNNFCSPLISAGCEVELVEIIPIEE
                     FKFPWNGARFFDAMPECVLDIPISIKKVALTKDRYDLVIIAWQPWFLSPSLPIISALK
                     DPKIMSVISQTPVITICGARNMWISAHKRIRNWIKDNGGIHVGSLVLRDRNNNLISGI
                     TIQYWLFKGRKDKMFGVFPKPGVSDDDIENCKNFGIDAYECLQNDEWNYLQQKWIKQD
                     GISVSKRLMFVEYRAIKIFTLWAKLIRRKQNRKLWINIFRIYLLFALFLLSPIVILLF
                     TLIIQPFFNKSLNKQKDFHEGVNLQP"
     gene            4263..5408
                     /locus_tag="PKY09_07250"
     CDS             4263..5408
                     /locus_tag="PKY09_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNB12691.1"
                     /translation="MLQEVYITRLSRFLPNKPILNDEMEQYIGLINEHSAKSKRIVLR
                     NNGIKCRYYALDKNGKATHTNSEMTAIAIRELFINRKEEIKDVDLLSCGTSSPDQWMP
                     SHAVMVHGWLTEARDMEVVSPTGNCCSGMHALKYIWLAVKTGQSKKAVVAGSERTSRI
                     MHHQSFEEEAKKLSQIEENPYISFDKEFLRWMLSDGAAAALIENKKNENGLSLRIDAI
                     EGVSYAHLVEPCMYSAADKMDDGSFVSYMDMSPSEIVTNSVLNIKQDVKLLSKYIVDL
                     GSDKLISMLKAHNLAGDKIDWFLPHISSEFFRDKMDQGFRSKGLVIPMEKWFTNLSEV
                     GNVGAGSIYLMLEELMRTNRLKQGEKIVLAVPESARFSYVFAILTVV"
     gene            5408..5821
                     /locus_tag="PKY09_07255"
     CDS             5408..5821
                     /locus_tag="PKY09_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12692.1"
                     /translation="MNKKDIPQDHSALVSYIKELCYAVGEDGQYTTANSTGWDVKTNA
                     LNLAWQDIEQRIITAYRQVKNHEVSPILYYMELRLMDISILSAYTGFWKWTIKRHLKP
                     AVFARLDNKALSKYALVFEITIEDLKTEKFNADKF"
     gene            5805..6812
                     /locus_tag="PKY09_07260"
     CDS             5805..6812
                     /locus_tag="PKY09_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HNB12693.1"
                     /translation="MQINFSHQQSAHCENGVTVNLLNYSGLKLTEPLVFGMGSGLFFI
                     YIPFLKVNNAPAFSYRTMPGLIFKRTCSRLGIKVIRKKFRSESEAQRILDDCLIHNQP
                     VGCQVGVYYLTYFPKEYRFHFNAHNLVVYGKTDDRYLISDPVMEDTTSLTEFELNRVR
                     FAKGPLAPKGQLYYPQIDAIVSDEKIVYAIKRGIKQNIRDMLYIPGPIAGVSGIKYVA
                     KKILKWHDSLGVKTTGLYLAQLVRMQEEIGTGGGGFRYIYAAFLQQAHAYIKNDSIIK
                     ISEQFTKAGDMWRDSAIQSAGIFKGRLTDRADFVNMHDRLMQIAEVEKDAFTKLSKIK
                     L"
     gene            6826..7566
                     /locus_tag="PKY09_07265"
     CDS             6826..7566
                     /locus_tag="PKY09_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB12694.1"
                     /translation="MDALTVSSLTYFYKNTEKAVLSNINLHVSEGEVFGVFGPNGAGK
                     TTFLSLITGILTCQSGVISILGKDAATQRKSINQLVGYVPQDFAFYEELSSLENLKFF
                     GAMYGLKEQSLDKRANELLDVFGLMHVAGRQVKEFSGGMKRRLNIAIGLLHKPQLLIL
                     DEPTVGVDVQSRLAIMSYLKKINSEGTTIIYTSHLLKEAEEFCTRIALIDKGKVIAID
                     TLANLIKQNQCKSLEEVFIKLTGNNYRD"
     gene            7566..8837
                     /locus_tag="PKY09_07270"
     CDS             7566..8837
                     /locus_tag="PKY09_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB12695.1"
                     /translation="MSPLAASIIKEFKVLSRDKTGLFLMFLMPLALALLMTVLQNSAF
                     KIVNENKVDLMLVNNDSNSLFFQNFRRGLEQTGMFNLSIHAGNSADSKFSEDKASIVL
                     VTIPKGYAFVLNQEIESKAEQALQLFGMLDSVSEREKLALKNLELSFDPVLQESYRYS
                     IKSTVISVVMAIQNEMLLRQLFYNVNQFQPDSTALAKILPNPLLLSESLLKENSVVNP
                     NATQHNIPAWTVFAMFFTVISLGGNIVREKRSGSFLRIKTLPVSITHLLIAKQVVFLM
                     VTVLQTFVLFSIGAFLFPILQLPPLIISSGFLLLFLATFSCGLCAVSYALCIGVYSNT
                     VEQANGFGAASVVILAAVGGIFVPLFAMPEVFKLFANISPFSWAMKAYYKAILEISSV
                     HKLLLNVLPLLLLTAILQLIAFIGLKRKNLL"
     gene            8834..9103
                     /locus_tag="PKY09_07275"
     CDS             8834..9103
                     /locus_tag="PKY09_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNB12696.1"
                     /translation="MTKEEIKPELKKHIIKYLNLLDLTPETIKDDQPLFGEGGLGLDS
                     IDSIELIVLLEREYGIKITDPKEGRKILIDVNTIADYIIQHKSAK"
     gene            9115..10308
                     /locus_tag="PKY09_07280"
     CDS             9115..10308
                     /locus_tag="PKY09_07280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNB12697.1"
                     /translation="MEDVCITGVGLVTAVGSNANETIDAIQRGKTGIGIPQYVKSRYA
                     SVIPFGEVGLSDTQLIDELAIGEKVVSRTQLLAHKAFNEALANSGLHLDDFKNAETSI
                     VLSSTVGGMYLTDELYADSNNLSKGSVYLSFYDCASIGIYLQQLTGAKGLINTINTAC
                     SSGANALIYGMRLLKNKRAKRVIVGGVDCLSKFTINGFNALNILSTKSCRPFDNNRDG
                     LNLGEGAGFLILESSDLAKEENVIACISGFANSNDAFHPSSLSDEGFGPMAAMRGALE
                     YAGLLPYEIDSVIAHGTGTPNNDKVEGLSIKKIFGESLPPVCSLKSILGHTLGAASAV
                     ESVLAVIGVKNGYIYPSRNFETPDKDTGLVPNLNFKKEKIKHCLLNSFGFGGNCSSLI
                     FSSVN"
     gene            10339..11349
                     /gene="obgE"
                     /locus_tag="PKY09_07285"
     CDS             10339..11349
                     /gene="obgE"
                     /locus_tag="PKY09_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNB12698.1"
                     /translation="MEKNFVDYVKICCRSGAGGSGYVHFHRDKKTAKGGPDGGDGGRG
                     GHIIVRGNKQLWTLLHLKYRKHILAEAGQKGGNALCTGADGKDEILEVPIGTIARDFE
                     TNKVLFEIEEDGQEMILAKGGRGGKGNAFFKTATLQTPRHAQPGEEGVEEWKVLELKL
                     MADVGLVGFPNAGKSTLLSVVSAAKPVIADYPFTTLVPNLGMVNYRDDKSFVMADIPG
                     IIEGAHKGKGIGLRFLRHIERNSILLFMIAADSKNIIEEYKILKNELRQHNPELLDKR
                     RVIAITKCDMLDDIMLKQMKKEVPTDKNPGNTKVPIVFISSVSGFNIQKLKDVLWQEL
                     NE"
     gene            11346..11945
                     /locus_tag="PKY09_07290"
     CDS             11346..11945
                     /locus_tag="PKY09_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNB12699.1"
                     /translation="MIGLLKSLDTQLFLLLNSWNCPAMDTFFYYATNRFVWIPLYVLL
                     FVLVIKRCKANSWKMLLMIIVLIVCSDQVSVLIKDNVKRYRPCHNLDIQEKVHKVNNK
                     CGGKYGFVSSHATNTMALCVFLVLSVFRKERLIVGILTFYVLLVSYSRIYLGVHYPGD
                     IIGGWILGLLIAVILFYILKRSIHELTAIKLATDREDRF"
     gene            12267..12647
                     /gene="crcB"
                     /locus_tag="PKY09_07295"
     CDS             12267..12647
                     /gene="crcB"
                     /locus_tag="PKY09_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNB12700.1"
                     /translation="MNIKAIFSIGVGCSIGGVLRYLLSLCVQSKTVSEFPVGTLLVNI
                     IGCFLIGVVYSFAEQSKMTVAWRLFIATGILGGFTTFSAFSMESFLMLRHGYYGMMAV
                     YVFLSVVIGICATICGFLLVRAIQ"
     gene            complement(12737..13372)
                     /locus_tag="PKY09_07300"
     CDS             complement(12737..13372)
                     /locus_tag="PKY09_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNB12701.1"
                     /translation="MIEPVISGFLLGCFLAILAGPVFFLIINISLRKGFSYASRVAMG
                     VLISDTLFIFIAYFGSNFILLLNNHRFIAGIISGILLIGFGIALILKRNSIPAEAITV
                     NEKTDSKSLYILKGFMINSMNPSVLFFWIGVSGTVSVKEHYTIHHLLLFYGSILITLF
                     SFDLFKAYIAHKLKSILTIKLLTWLNRITGMALLGIGLYTLIVACWQAFAK"
     gene            complement(13392..14939)
                     /locus_tag="PKY09_07305"
     CDS             complement(13392..14939)
                     /locus_tag="PKY09_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNB12702.1"
                     /translation="MTLIGRIIKASIDLRGKIEIAPFSPFISQKRVLKKLIRKAQFTM
                     FGEAYNFQGMLGNKNFVTKFRAVVPVHDYNSIFSNWWNKSLHDQENVCWPGKVRYFAL
                     SSGTSGSSSKHIPVTKEMSKAIQKASLKQILSLSKYDLPFSTFEKGILMLGGSTHLHQ
                     QGSYFEGDLSGITASQIPFWFQHFYKPGRKISREKDWHAKLDEITHKAKDWDIGIIVG
                     VPAWVQLLIEKILEHYKLKNIHELWPNLEIYVHGGVSFEPYKKGFSKLLGKQIYYIET
                     YLASEGFIAYQDEPNTRGMRLIINNGIFYEFIPFNEDNFDADGELKHKPKTLLLNEVK
                     ANEPYAILLTTCSGAWRYLIGDVITFTNIEKCEIQIIGRTKHYLSLCGEHLSVDNMNR
                     AIELVSDELNIDIPEFAVAGIPYHSLFAHKWYVGCDTYNDSENLKKLIDEKLKILNDD
                     YRVERRSALKEIFVEIIPTSWFYKWMELQGKIGAQNKFPRVMKNKVFEDWEKFVASQH
                     KNINQEV"
     gene            complement(15037..>15975)
                     /locus_tag="PKY09_07310"
     CDS             complement(15037..>15975)
                     /locus_tag="PKY09_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12703.1"
                     /translation="LTVTSTGGNAGFVSTSTFETNPANLPFPPGVGHVNSAATGADNS
                     ADCVDRFWKINQSGSSTTFNAVFSAPVAEFPAIAGTYKAQHYNYAGGFWDNPFQGAQS
                     YNAVGAVRSVTVTGLSTANDWWVLVNENTPLPVELLSFDANCINNRHQIKWTTASELN
                     NDFFTIEKSSNGNEFTFFRKIQGSGTINDTKTYTVSAEDGIAEYYKLKQFDFNGTMHE
                     LKTIYAPSCQNDMFKLLSVNQVEEVVNSIISSPDNVKAEITLFDLKGILVFKEEVSLT
                     TGINSVRLPINLAKGLYYFQIINDNQVTQGRKFLVN"
     assembly_gap    15976..16075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16076..19119)
                     /locus_tag="PKY09_07315"
     CDS             complement(<16076..19119)
                     /locus_tag="PKY09_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12704.1"
                     /translation="MKDFQKIISFFMVTVLFVFSSYRSEALIFQSVASGNFNTLIWNQ
                     PGLYPTAGDTVVINTGTTVTIIGINTDILGSVTVNAGGTLTIANNAVAQLVFAGNLVV
                     SGTLINNGRIDMTVPGNFVLGNGAVYDHNPRINIAVDEKVFENGVESFSPSSTLIIRK
                     WASTALPLIGPTRITSDIGNLVTSVSGATWEQKGQFSPNRIKGSLLVTDGVLRMDDGS
                     GATTTLTLNDVTLRGNSAIIFQEGNNRNLTLTMGNFTDSSTLSTQPTIIMNMSLGALN
                     LTVNGHFITRHDFIAVYDSTFPIRSASVIANIMGDLFIQGSGNKFDFCKQVNAPLTLT
                     VGGNTKIAGNPNYVRFVDSGNGFLNYTTGDLNISAGLKNVFLGGNDALDASNAPLFPL
                     PTSYATINVLNDFNLSGVTNTTIMLSPGNVARTMLIVGHDLTSSASTVNFVVSNSIGI
                     VNVGINSNLMLNGGSMNVQANASSNAIDSISVTRSFVFNSTNPSNFFRGNSGAGNTYI
                     HIFGDFNLMNSGTATNQGVCGTYLNNGNLEFHVGGNFNLNAGAFYGIQDGTGRNTFSV
                     DGTLNQSGGFFKGTHAPNIFTAGKSIFTLGAFNFTGGNYIQYNAANDSVICTVNGDFT
                     VNYSAVSNLVSFIPYLHPVYSNTAPLKLTVSGVMSFDGANGTFYSSVTQGNEVITLDS
                     NLIFNNGNNSFNNYPSAVVTTTHNVDITIAGNLTCNNGINNFSAGRGDLTGTINGDLI
                     ISGGTLSLKSNSAYSPIVINVLGGFSMSNGNFFYTNNATVPPITTTVTINSDDDNTGD
                     FVHSGGTINFVNNTLNPTVQSLTIKSPNILLGPSGTIKRNGAGTIAARGALDFARTGT
                     TLFTRLNGHTIDHSVITILSGTTLEVISGDVQLASYVEEFAGQNLPMLYVSSGATFTL
                     RNNSSVYTTGNFLKSTVQGASGSRIRIQHTNGFYSGFSSAAINSSGNMGFILSASGTV
                     EYFGDDNQIVTGIGMGNAVGGNQKYGILEINFGGTPNTEYVYP"
     gene            complement(19503..20171)
                     /gene="tsaB"
                     /locus_tag="PKY09_07320"
     CDS             complement(19503..20171)
                     /gene="tsaB"
                     /locus_tag="PKY09_07320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNB12705.1"
                     /translation="MALLLHLETSTDTCSVCISDNETIIDCKETHNSKSHASLLAVFI
                     DELLKQNQLTYKNIDAVVISCGPGSYTGLRIGVATAKGLCFALKVPLISVPTLQSMAN
                     NALKKCHGQLLLCPMIDARRMEVFTALFTPQLDYVEKENAAIINDHFQHDYLLKNKIC
                     FFGNGAEKLPESIKTNSNCTILDDFKMSATGLVTLAFKKFTQQTFEDLAYFEPFYLKD
                     FVGK"
     gene            complement(20177..21550)
                     /locus_tag="PKY09_07325"
     CDS             complement(20177..21550)
                     /locus_tag="PKY09_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB12706.1"
                     /translation="MSKKNKTLRNIIIIAGVVIIALVIAKKAGWLGEENITNVSTEKA
                     SLQTITESVSASGKVQPEVEVKISSDVSGEIVEMLVKEGDVVKKGQLLCKINPEIYIS
                     SLDRAVAGLNTTKANYESSKSRLSQAESQLVKAEIAYTRNKKLFEEKVISASDFEAIK
                     SAYEVAKAEVDASKQGVSGAQYNINSGNATVREAKENLNKTEIFAPVNGTISKISKKK
                     GERIAGTSFTDGTEILRISNLQEMEVKADVGENDIIRIHLNDTTLVEIDAYSPRKFKG
                     IVTEIANSPNESLTSTEQVTNFAVKIRILRESYADLIPSDHPEYSPFRPGMSATVEIQ
                     TKKVSNVVSVPIKAVTTRDTTIRASDKAVIRKSDDTPDVKVVATNNTDETPVKEIVFV
                     NDNGIAKMHVVKTGIQDNNFIEILEGIKVGDEIITDPYQAVSKKLVDGDKIKVVPQNQ
                     LFIEEQK"
     gene            complement(21560..22978)
                     /locus_tag="PKY09_07330"
     CDS             complement(21560..22978)
                     /locus_tag="PKY09_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB12707.1"
                     /translation="MKHNIIIFLFIFFFSEVNAQKIWSLKDCIDYALNNNISVQQSII
                     ASKIAAATYQQSVGSLFPSINASSSLNYNYGRSLDPTSYQYVDQTFQSGGASLNGNLP
                     IFQGLELQHNLRKSSIDFDASKLDVRKVQNDIALSVATAYLQILYSNEQLKATNDRID
                     AVTKERNRAKILVDNDLLAEGSLLDADALLATEEFNKASAENLLTSSRLTLIQLMQLE
                     GASDIVIETPTLEIPSQESLSLSSESVYNEALKNLPEVKASELRLKSAKESLGIARAA
                     RYPKLSAFGSLSTNYSNQATSLDGSPVFTGYVPSGAVTSSGEQVLQPVFNYKYIDTPL
                     KDQLNNNFGKAIGFNLSISLFNGWAINSNIKRSKLSFENARLNALQTKNTVYKNVQQA
                     HADAIAALNKYAAANKSVAAQEKAFLYTEKKYNAGLLNSIDYVNARNNYTKSQSDLIQ
                     SKFDLILKIKVLDYYLGKPLSF"
     gene            23190..>24399
                     /gene="ffh"
                     /locus_tag="PKY09_07335"
     CDS             23190..>24399
                     /gene="ffh"
                     /locus_tag="PKY09_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNB12708.1"
                     /translation="MFENLSDKLEKAFKNLKGQGKITEINVAETLKEVRRALLDADVN
                     YKVAKQFTDTIKEKALGQNVLTAVSPGQLLVKIAHDELATLMGGQMTDIHLKGNPSVI
                     LMSGLQGSGKTTLSGKLANFLKTKKGKTPLLVACDVYRPAAIDQLKVLGEQIGVEVYS
                     EIENKNPVEIARHAIKQAKQNGNNVVVVDTAGRLAIDQQMMNEIAALKNAIEPDEILF
                     VVDSMTGQDAVNTAKAFNDRLNFDGVVLTKLDGDTRGGAALSIRSVVNKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVTLVEKAQEQFDEKQAAELQKKFAKNQFNFNDFLS
                     QLQQIKKMGNIKDLVSMIPGVGKAVKDMDIDNNAFKSIEAIIQSMTPAERANPDLING
                     NRKKRIAAGSG"
BASE COUNT         7766 a         3834 c         4724 g         7975 t
ORIGIN      
        1 aatcccgttc gacttgcatg tattaggcct gccgctagcg ttaatcctga gccaggatca
       61 aactcttcgt tgtagtttgt cttttaattc ttttcttggt cgaaaccaag aagtaagaat
      121 ataatgctct gggattttat tcatgtcctg attttgtgct attttaaaat caggtcaggc
      181 ttgttatttc cttttaatct tcaaagaacg tttaatatat tttgataaac aaaaatatcg
      241 ttttcaaaat gggagtgcaa aagtagaact tgttttacaa ttacacaaat ttattttaaa
      301 ctttttttat tttattttta actccgttga gttaaatccc tttttggtta ttttggggct
      361 gcaaaggtaa ctctgtttat ggatatgcca aataatattt caatcttttt tattaacata
      421 gctttatatt gtgttaatat attggtaaac aatataataa attagatatt tgcagtaatt
      481 tatgtcagaa agcagccaaa aacactttag ttttaaagaa tttaaagtgc atcaggagca
      541 atgtgcaatg aaggttggca ctgatggagt ccttttaggt tcttgggttg aatgtaatta
      601 tagtgtatcg aatgtattag atattggtac aggaaccgga ttgttggcat taatgttggc
      661 tcaaaagtgt agtgctttga ttgatgcaat agatatagat agtgatgcat gcaatcaggc
      721 aaaggaaaat ttttatcatt caaagtggag cgacaggctt agtgttattc ataaacctat
      781 acaagagttt gcagcaattt caaataagaa gtatgattta attgtcagta atccaccttt
      841 ttttatcaat gcgaaaaaat cacagcaatt aaatagaaat gtggcgaggc acttagatga
      901 ttcttttacc atggatgacc taataacatc agtagcaaag ttgttgagtg attcgggaaa
      961 gttttttctt atacaacctg taaaagaagg aagcattttt atggaagaaa gtgagaagcg
     1021 cggattgtat gtcaataata taacatgggt aagaaccaaa gaggataagg aagagaaacg
     1081 gctgctttta aatttaagcc gaaataagaa aaagattatt actgattatt tatctattca
     1141 aacaaaggat ggaaaatata ctgatgagta tattaaatta acggaggagt attataccca
     1201 tttgcctgaa cgttctttct gataaatata tactcaatat agaatattca gtgcttttct
     1261 ttaaattctt atggttaatt tctttggtgg ttgtcttact ataaagttta aatattaaac
     1321 atttttgaag aaaatgcgta gttgtcttct atttcttgaa tagaaattat ccttgctcca
     1381 ctttaaaaat tatgttaagc tcggataatt cattcaacac aggttcataa atttcaggaa
     1441 taacaggaat tgtcacacct ttggttttta tttcgccatt gagaataagt ttggctgcta
     1501 ttgccattgg caatcccact gtttttgaca tggcagttgc ggtttcattt tcgcctttta
     1561 cacttagtga tgaaatgtgt gtgtgggttt tactgttcag tttgtattca aaaatatgtt
     1621 gcattactac aaggtctaaa tcgccatgct gcaatttcca ttttgattcc aaaagattca
     1681 aaagtacttc tgccggtgta ccattactaa taggaataag cgttttttcc agaatgcctg
     1741 tccaagtaat tctgttccag tcatcggaag agaggttgag ataatgaaat tgtgtttgca
     1801 gcttttgcaa taaaggtttg ccatcatcgg gtaggtaaga tgaaatccag tcgtgataag
     1861 taagtgatga ggtatttatt ttataggtat cgtctgtaat gccgagatgc acaaataagt
     1921 tccaagatga acaatatcct atataacgaa gtgttccacg cagcagggtt gaaatattca
     1981 gtagattgta agtttcaata taagcatttg aatttctgtt agcataacct tcaaactgac
     2041 caattcctga aatatttatt ttttctgttt gtgaaaacaa gcgatggtat ggtgtgaatt
     2101 tattgcttcg attttgccta taaagcgctg taccttgtcc tgccagaata acattcttag
     2161 gattccatgt aaatttatat ccccatggat tattgtttga ttcaggtgat attaaacctc
     2221 cggtgtagga tttaaatgaa ataatttcag ctcctttaga ttgtaatgaa tgtataattt
     2281 gcattgccga catatgatct attcctgggt caaggccaca ttcatttaga aaaatcagat
     2341 ttttattttt cacatcggca tcgaacgact tcatttgttc ggaaacgtag gatgctgtaa
     2401 cgagattttt tccggcaaca agacattcaa cagcagcaat agcatgaagt gaaggaggga
     2461 gtagagaaat tacaatgtca gctttattga tttctgattt tcgttctgat tcgtcattta
     2521 tattaaaggc aattgcattt gacgaaggat ggtttcttgt tctggatttt gctaattcaa
     2581 gagaggcatc ggcaacagta acatgccaat tttgttcctg agcattaaaa agaagataat
     2641 taataagatg ccaggaagat tttcctgctc ctaaaacaag tattttgcgc atagtaaaaa
     2701 tttaaatgtg tgcaaaagta caaattgaat ttttgaacgc attttggcca tgctaatatc
     2761 atgttaaaag gtgtcttatt catgccctaa aaaaacatac ttttacagcg gtttatttgt
     2821 acaataatta aagtgaaaaa tatttgattg ttattagtag ataaattttt cataatctga
     2881 gatgagagta atattaagtt gttttttgtt tatcattatt ggtttaaagt ctgatgagga
     2941 catgaaattg tttgccagag ttgtggagaa tttgtcaact accgatattg ttgttctgaa
     3001 caaactaatt gacgaagtta gaatttctaa taccagtatt tcacgtgcat gccatgggac
     3061 attgctgatg aagaaagctg attttgtgaa aagaccctca gagaaattaa gtttgtttaa
     3121 agagggtaga aagttgctgg agacagaaat aaagaaagat agtgctaatg taaattttag
     3181 aatattacgg ttaattattc aggaaaactg ccctgtattt ttaaaataca atactaattt
     3241 agatgacgat gctgtcttcg ttcaaaggaa tttcgataaa ctttcaaata aaatacgtga
     3301 tttgctgatt acctattcta aaaactctaa acatttaaag ctaaaagaaa gtgagtaagc
     3361 aaattttggt tttatattat tcacaaaccg gtcagttgga gcagatagtg aataacttct
     3421 gctcaccact tattagcgct ggatgtgaag ttgagttggt tgaaattatt ccaatagaag
     3481 agtttaaatt tccatggaat ggggcacgat ttttcgatgc catgcctgaa tgtgttttag
     3541 atattcctat ttcaataaaa aaggtagcgt taacaaaaga ccgttatgac ttagttataa
     3601 ttgcctggca accttggttt ttatcgcctt cattacctat aatttctgcc ctgaaagacc
     3661 caaagataat gtctgtaatc agccaaactc ctgtaataac aatttgtggt gcacgtaata
     3721 tgtggatttc agcacataag agaataagga attggattaa agataatggg gggatacatg
     3781 ttggtagtct tgttttaaga gatagaaaca ataatttaat aagcggaatt actattcaat
     3841 actggctttt caaaggtaga aaagacaaga tgtttggtgt attcccaaag ccgggggtta
     3901 gtgatgatga tattgaaaat tgtaaaaatt ttggcattga tgcatatgaa tgtttacaaa
     3961 atgatgagtg gaattacttg caacaaaaat ggataaaaca ggatggaata tctgttagca
     4021 agagattgat gtttgtagaa tacagggcaa taaaaatatt taccttatgg gcaaaactga
     4081 taagaagaaa acaaaaccgt aaattatgga taaatatttt tagaatttat cttttatttg
     4141 ctttattttt actttcaccg attgtaattc tgttgtttac actaataata caaccatttt
     4201 ttaataaatc attaaataag caaaaagatt tccatgaagg tgtaaatttg cagccctaag
     4261 ttatgttaca agaagtttac attacccgac tatcaaggtt tttgcccaat aagccaatat
     4321 tgaatgatga aatggagcaa tatattggtt tgattaatga gcattcagcc aaatcaaagc
     4381 gaattgtttt gcgtaataat ggtataaagt gtcggtatta tgctttagat aaaaatggaa
     4441 aagcaaccca taccaattct gaaatgacgg caatagcaat cagagaacta tttattaata
     4501 gaaaggaaga aattaaggat gttgatttgt tgagctgtgg aacatcatca ccagaccaat
     4561 ggatgccatc gcatgctgtg atggttcatg gctggctaac tgaagcaaga gatatggagg
     4621 tggtgtcacc aacaggtaat tgttgttccg gaatgcatgc tttaaaatat atatggctgg
     4681 cagtaaaaac aggacagtca aaaaaggccg ttgttgccgg ttctgaacgt acgtccagaa
     4741 ttatgcatca tcagtcgttt gaggaagaag ctaaaaaact ttcacagata gaggaaaatc
     4801 cgtacatttc tttcgataag gaatttttaa gatggatgtt gagtgatgga gctgcagcgg
     4861 cattaataga aaataaaaag aatgagaatg gattgtcttt gcgaattgat gccatagaag
     4921 gagtgtctta tgctcatttg gtagagccat gtatgtattc tgcagccgat aaaatggatg
     4981 atgggtcatt tgtgagttat atggatatgt ccccttcaga gattgtgacc aattctgtat
     5041 taaacataaa acaagacgta aagttgttga gcaaatatat tgttgattta ggttccgata
     5101 agttgatttc gatgcttaaa gcacacaatt tagctggaga taaaatagat tggtttttgc
     5161 cacatatttc aagcgaattt ttcagagata aaatggacca gggttttcgg tctaaaggtt
     5221 tggttatacc aatggaaaaa tggtttacca acttgtctga agttggtaat gttggagcag
     5281 gatctattta tctgatgctt gaagaattga tgcgaacaaa tcgtctaaag caaggtgaaa
     5341 aaattgtatt agcagttccg gaaagcgcgc gtttttcgta tgtgtttgcc atattaacag
     5401 ttgtttaatg aataagaaag atattccgca agatcatagt gcattagtca gctatataaa
     5461 agagttgtgt tatgctgtag gtgaagatgg gcaatataca acggctaaca gtacaggatg
     5521 ggatgtaaaa acaaatgcat tgaatttagc ctggcaggat attgaacaaa gaattattac
     5581 cgcatacaga caagtgaaaa atcacgaagt aagtccgatt ttatattata tggaattgcg
     5641 cttaatggat attagtattt taagtgcata taccggattc tggaagtgga ctataaaaag
     5701 acatttaaag ccggcagttt ttgcaaggct cgataataaa gctttatcta aatatgcttt
     5761 agtttttgaa ataacaattg aagatttaaa aacagaaaaa tttaatgcag ataaatttta
     5821 gtcaccaaca atcggcacat tgcgagaatg gtgtaactgt aaatctttta aattattcag
     5881 gactgaagct taccgaacca ttagtgtttg gtatgggctc aggattattt tttatttata
     5941 ttccatttct taaagttaat aatgctccgg ctttttctta tagaacaatg cctggattaa
     6001 tctttaaaag aacgtgcagc cgtttaggaa taaaggtgat aagaaaaaaa tttcgttcgg
     6061 aatctgaagc gcagagaata cttgacgatt gtcttataca taaccaacct gttggctgcc
     6121 aggttggagt ttactatctg acatatttcc ctaaggaata tcgtttccac tttaatgctc
     6181 acaaccttgt tgtttatgga aagacagatg acaggtattt aatcagcgac cctgtaatgg
     6241 aagatacaac atctttaaca gagtttgagt tgaatagagt gcgatttgcc aaagggcctt
     6301 tggcacctaa ggggcaattg tattatccgc aaatagatgc aatagtttct gacgaaaaga
     6361 tcgtgtacgc aataaaacga ggcataaagc aaaacatcag agatatgctt tatatacccg
     6421 gacccattgc cggtgtcagt gggataaagt atgttgcaaa aaaaattctg aaatggcatg
     6481 atagtttagg tgtaaaaacc acgggtttgt atttggcgca attggtaagg atgcaagaag
     6541 aaataggtac cggtggtggt ggatttaggt atatctatgc agcatttttg caacaggctc
     6601 atgcatatat aaagaacgac agtatcatta aaatatcaga acagtttaca aaggcaggtg
     6661 atatgtggcg tgattcagca atacaatctg caggaatttt taaaggccga cttacagata
     6721 gagctgattt tgtgaatatg catgacagat tgatgcagat tgcagaagtg gaaaaagatg
     6781 cttttactaa attgtcaaaa ataaaactgt gaaagcataa accttatgga tgcactcact
     6841 gtatcatcat taacatattt ttataaaaac actgagaagg cagtattgtc taatattaac
     6901 ctacatgtca gtgagggaga agtttttggt gtgtttgggc caaatggtgc cggcaaaaca
     6961 acatttttat cattaatcac aggtattttg acatgtcagt cgggtgtgat ttctatttta
     7021 ggaaaagatg cagctacgca acgaaaaagt atcaatcagt tagttggata tgtaccacaa
     7081 gattttgcat tctacgaaga gttgagttcg ttagaaaatc tgaaattttt tggtgccatg
     7141 tacggtttaa aggagcaatc attagataaa agggcgaatg aattattgga tgtatttggt
     7201 ttgatgcatg ttgctggaag acaggttaag gaattttccg ggggtatgaa acgcagacta
     7261 aacattgcaa ttgggttgct gcataagcca caattattga tacttgatga accaactgta
     7321 ggagttgatg ttcaaagcag attggcaata atgagttatc tgaaaaaaat aaattctgaa
     7381 ggcactacaa ttatatatac ttcccattta ctaaaggagg ctgaagagtt ttgtactcgc
     7441 attgcattaa ttgacaaagg aaaggtgata gcaatagata cattggcaaa tttgataaaa
     7501 cagaatcaat gtaagtcact tgaagaagtg tttattaaat taaccggaaa taattacaga
     7561 gactaatgag tccattagca gcatcaatta ttaaggagtt taaagttctt tcgagagata
     7621 agaccggact gtttttgatg tttttaatgc ctcttgccct ggcattactg atgactgttt
     7681 tgcaaaacag tgcatttaag atagtcaacg aaaacaaggt tgatttaatg ttagttaata
     7741 atgattctaa tagtctgttt tttcagaatt ttcgcagagg acttgaacaa acgggtatgt
     7801 ttaatttaag cattcatgca ggaaatagtg cagacagtaa gttttcagaa gataaagcat
     7861 ctattgttct tgttacaata cctaaaggtt atgcctttgt tcttaatcag gaaatagaat
     7921 caaaggctga acaggcgttg caattgtttg gcatgttaga ttcagtttca gaaagagaaa
     7981 aattagcgtt gaaaaatctt gaattgagtt ttgatcctgt acttcaggag tcgtatcgtt
     8041 attcaattaa gtcaactgtt atatcggttg tcatggctat tcagaatgaa atgttgttaa
     8101 ggcaactttt ttataatgtc aatcaatttc agcctgattc aactgcattg gctaaaattt
     8161 taccgaatcc actgttgctt agtgagtcgt tgctaaagga aaatagtgtc gtaaacccaa
     8221 atgcaacgca gcataatata ccggcatgga cagtgttcgc catgtttttt acggtgatat
     8281 cattaggagg aaacattgtt agagagaaaa gaagcggaag ttttttacga attaaaacat
     8341 tgcctgtatc aattacccat ctattaattg ctaaacaagt ggtcttttta atggtgaccg
     8401 ttctacagac atttgttttg ttttcaattg gtgccttttt atttccgata ttacaattac
     8461 caccattaat aatttcatca gggtttttgc ttttgttttt ggctacattt agttgtgggt
     8521 tatgtgcagt tagttatgct ttgtgtattg gagtatattc caacaccgtt gaacaagcta
     8581 atggatttgg tgctgcctct gttgttattc ttgctgccgt aggtggtata tttgtgcctt
     8641 tgtttgctat gcctgaagta tttaaattat ttgctaatat ttcgccattt agttgggcaa
     8701 tgaaagctta ttataaagca atacttgaaa tatcatcagt ccataagcta ctgctaaatg
     8761 ttttaccttt gcttttgctg actgcaatcc ttcagcttat tgcctttatc ggattaaaaa
     8821 ggaaaaattt attatgacaa aagaagaaat caaacctgag ttaaagaagc atattattaa
     8881 atatttaaat ctgcttgact taactcccga aactattaaa gatgaccaac cactatttgg
     8941 agagggtgga ttagggttag actctataga ttctattgaa cttattgttc ttttagaacg
     9001 ggaatatggt ataaaaatta ctgaccctaa ggaaggaaga aaaattttga tagatgtcaa
     9061 tactattgct gattatatta ttcaacataa gtctgcaaaa taatttttag cattgtggaa
     9121 gatgtttgta taacaggcgt tggtttggtt acagccgttg gaagcaatgc aaacgaaacc
     9181 atagatgcta ttcaaagagg aaaaacagga ataggaattc ctcaatatgt aaaaagcaga
     9241 tacgcttcgg ttattccatt tggagaagta ggtctgtctg acacgcagtt aattgatgaa
     9301 ttagccattg gagagaaagt tgtcagccgt acgcaattac tggctcataa agcatttaat
     9361 gaggcgcttg ctaatagtgg gttgcatctt gatgatttta agaatgccga aacatcaata
     9421 gttctttcaa gtactgttgg tggcatgtat cttacagatg aactatatgc agattcaaat
     9481 aatctttcta aaggatctgt ttatctttct ttctatgatt gtgcttcgat tggaatttat
     9541 ttacagcaac ttaccggagc aaagggttta attaatacta ttaacacagc atgttcctcc
     9601 ggtgcaaatg ctttgattta tggaatgagg ctattaaaaa ataaacgtgc taaacgagtt
     9661 attgttggtg gagtagattg tctttcgaag tttacaataa atggttttaa tgcattgaat
     9721 atattgtcaa caaaaagttg cagaccgttt gataacaatc gtgatggatt gaatcttggt
     9781 gagggtgcag gttttcttat actcgaatct tcagacttag caaaagagga aaatgtaatt
     9841 gcctgcatca gtggttttgc taattcaaac gatgcctttc atccgtcatc cttgagtgat
     9901 gaaggttttg gtcctatggc ggcaatgcgt ggtgcgttgg aatatgccgg gttgttacct
     9961 tatgaaattg acagtgttat tgcgcatggt acaggaacac ctaataatga caaggtagag
    10021 ggattatcta ttaaaaaaat atttggagag tcactgccac cggtctgttc tttaaaatct
    10081 atcttaggac atacattagg tgctgcatcg gctgttgaaa gtgtactcgc tgtaattgga
    10141 gttaaaaatg gatatatcta tccaagcaga aatttcgaaa ctcctgataa ggataccgga
    10201 cttgtaccta acttgaattt caagaaagaa aagataaaac attgtcttct taattcattt
    10261 ggatttggag gcaattgtag ttcattgatt ttttcctctg taaattgaaa ataaatcaga
    10321 ttaaaccagt taaatcaaat ggagaaaaat tttgttgatt atgtaaaaat ttgctgtcgt
    10381 tcaggagcag gaggttcggg ctatgtacat tttcacagag ataagaaaac tgccaagggg
    10441 ggacctgacg gaggcgatgg cggacgtggt ggacatatta tcgtacgagg caacaaacaa
    10501 ctttggactt tattacattt gaaatatcgc aaacatattc ttgctgaggc cggacaaaaa
    10561 ggaggtaatg cactttgtac cggagcagat ggcaaggatg aaatactgga agtgccaata
    10621 ggaacaatag ccagagattt tgaaacgaac aaagttctgt ttgaaataga agaagatggt
    10681 caggagatga ttttagctaa aggtgggcga ggcggcaaag gcaatgcttt ttttaaaaca
    10741 gcaacactac aaacaccacg tcatgcgcaa ccaggagagg aaggtgttga agagtggaaa
    10801 gtattagaat taaaattaat ggccgatgta ggattggttg gatttccaaa tgccggaaag
    10861 tcaaccttgc tttctgtagt ttctgctgcc aaacctgtaa ttgcagatta tccatttacc
    10921 acattggttc ccaacttagg tatggtcaac tatcgtgatg acaaatcgtt tgtgatggca
    10981 gatatccccg gcatcattga aggagcacac aaaggaaaag gaattggtct gcggttttta
    11041 agacatattg agcgcaactc catcttattg tttatgattg ctgccgattc aaaaaatatt
    11101 attgaggaat ataaaatatt gaaaaacgaa ttacggcaac acaatccgga gttgttagat
    11161 aaaagaaggg taatagccat tactaaatgt gatatgttgg atgatataat gttgaaacaa
    11221 atgaagaaag aggtgccaac tgataagaat cccggaaaca ccaaagtgcc tattgttttt
    11281 atttcttctg tttcgggttt caatattcag aaacttaaag atgttttgtg gcaagagctt
    11341 aacgaatgat tggtctgctt aaatcattag atacgcagtt gtttttactg ctgaattcat
    11401 ggaactgtcc ggccatggat actttttttt attatgcaac aaacaggttt gtctggatac
    11461 cgctttatgt acttttgttt gtgttggtga taaaaagatg caaagccaac tcctggaaaa
    11521 tgttgctgat gattatagtt ctcattgtat gtagcgatca ggtttctgta ttaattaaag
    11581 acaatgtgaa gcgttacagg ccatgccata accttgacat tcaggaaaaa gtacataagg
    11641 taaataacaa gtgtggtggc aaatatggtt ttgtttcatc gcatgctacc aataccatgg
    11701 cactttgtgt ttttttggta ttgtctgttt tcaggaaaga gcgtttgatt gttggcatac
    11761 tgacatttta tgtattactt gtttcgtaca gcagaatata tttgggagtc cattaccccg
    11821 gagatattat tggaggatgg atattgggct tgctgattgc agtgatactt ttttacattt
    11881 tgaagagaag tattcatgaa ctgacagcaa taaaattagc tacagataga gaagatcgtt
    11941 tttagttatt aaggcattgt gtgtgaactg aaagattctt tttcattctt acttcaacaa
    12001 cactttgcta ttatataccc tgatatcttt tttgtccaca caaagatggg aactccattt
    12061 tagaattcca agaaaaaatc ggactcactt aaagcgcact tatcaaaaaa aatagcttga
    12121 acaactgatt acctaaaaaa ttaaacttca tatcttctaa agaagtattt ttgtgtgaaa
    12181 ctttatattg gaaggcagtt cttaaataaa ttaaattgtg atttattaaa tgcaattttc
    12241 aaaagccgaa agatggtatt tacgacatga acattaaagc aattttttca atcggagtgg
    12301 gatgttctat cggtggggtg ttgcgctatt tactttcact atgtgttcaa tctaagacag
    12361 tttcagagtt tcctgtaggg accttgcttg tcaatattat tggttgcttt ttaattggtg
    12421 ttgtctatag ctttgccgag caaagtaaaa tgacggtagc atggcgttta tttattgcaa
    12481 caggaattct tggcgggttt acaactttct cggctttttc aatggaatcg tttttgatgt
    12541 tacgtcatgg ctattatgga atgatggctg tatatgtttt tctcagtgtg gtgattggta
    12601 tttgtgctac gatatgcggt tttttgctgg taagagcaat ccaataataa tttatataat
    12661 aactgaattt caaaattggt tgcacataaa agaatgtaat caactatctg taaggttatt
    12721 ttattacggc tgatttctat tttgcaaaag cctgccaaca ggcaactatc agggtatata
    12781 agccaattcc aagtaatgcc atgcctgtaa tcctatttag ccaggtgagt aattttattg
    12841 tcaaaataga ttttaattta tgtgcaatgt atgctttaaa aagatcgaat gagaaaagcg
    12901 tgattaatat agagccataa aacaacaaaa ggtgatgaat agtatagtgc tctttaacag
    12961 aaactgttcc cgaaacacca atccagaaaa ataatactga tgggttcatg gagttaatca
    13021 taaacccctt taatatataa agactcttgc tatctgtttt ttcgtttact gttatagctt
    13081 ccgctggaat gctatttctt ttaagtatta aagctatgcc aaagccaatg agtaaaattc
    13141 ctgaaatgat tccagcaata aatctgtgat tgttgagaag taaaataaaa ttactgccaa
    13201 agtaggcaat gaaaataaag agtgtgtcac tgataagtac acccattgct actctgcttg
    13261 catagctaaa gcctttacgt aagctgatgt ttatgataag aaaaaaaacc ggaccggcaa
    13321 gaattgccaa aaagcaacct aacagaaatc cacttattac aggctcaatc aaaattgtag
    13381 tttataaaaa attaaacttc ttgatttata ttcttatgtt gtgatgcaac aaatttttcc
    13441 caatcctcaa aaactttatt tttcattact ctggggaatt tgttttgtgc acctattttg
    13501 ccttgtagtt ccatccattt ataaaaccat gatgttggaa taatttcaac aaaaatttct
    13561 ttcaatgcac ttcttctctc aaccctataa tcgtcattaa gtattttcaa tttttcgtca
    13621 ataagttttt tcagattttc tgagtcatta taggtgtcac agccaacgta ccatttgtgt
    13681 gcaaacaatg aatgataggg gatacctgca actgcaaatt ccgggatatc aatatttaat
    13741 tcatcagaaa caagttcaat agctctgttc atgttgtcaa cagagaggtg ctcaccacat
    13801 aaactcagat agtgttttgt tctgcctata atctgaattt cacatttttc aatattggta
    13861 aatgtaatta catcgccaat caaatatcgc catgcaccgg aacatgttgt caatagtatg
    13921 gcataaggct catttgcctt aacttcattt aataataaag ttttcggttt gtgttttaat
    13981 tcgccatctg catcaaaatt atcttcgtta aaagggataa attcataaaa tattccattg
    14041 tttatgataa gcctcatacc tcttgtgttt ggctcatcct gataggcaat aaatccttca
    14101 gaggctaaat aggtttcaat gtaataaatt tgtttgccta agagtttgct gaatcctttt
    14161 ttataaggct cgaatgacac gccaccgtga acataaattt caaggttcgg ccaaagctca
    14221 tgaatgtttt taagtttgta atgttcaagt attttttcaa tcaaaagttg aacccatgcc
    14281 ggaacaccta ctatgattcc aatgtcccaa tcttttgcct tatgcgttat ttcatccagc
    14341 tttgcatgcc agtctttttc tctggagatt tttcttccgg gtttataaaa gtgctgaaac
    14401 caaaatggaa tctgacttgc tgtaatgcca ctaagatcac cttcaaaata acttccttgc
    14461 tgatgtaaat gtgtactacc acctagcatt aatattcctt tttcaaaagt tgaaaagggc
    14521 aggtcgtatt tagataaaga aagtatctgt tttaagctgg ctttttgaat ggctttagac
    14581 atttctttgg ttacaggaat gtgcttgctt gatgaaccgg aagttccgga acttaaagca
    14641 aaataacgga ctttgccggg ccaacaaaca ttttcctgat catgtaaact tttattccac
    14701 caatttgaaa aaattgaatt ataatcatga acaggaacta ctgcccggaa tttggttaca
    14761 aaatttttat taccaagcat tccttgaaag ttgtatgctt caccaaacat ggtaaactgg
    14821 gctttcctga ttaatttttt cagtacacgc ttctgcgaaa taaaaggaga aaagggtgca
    14881 atttcgattt tccctcggag atcaatactt gccttaatta ttctgccaat caaagtcatg
    14941 ctaaaatgtt tgcaaggcta aaaataggaa atactttgaa atgcaataaa aaaacccatg
    15001 aattgacatg ggtttttata aaattataat tattctttag ttgactaaaa actttctgcc
    15061 ttgggtaacc tgattgtcat ttataatctg gaaataatat aaacctttag caagattaat
    15121 aggaaggcga actgaattga tgccagttgt aagagaaact tcttctttaa aaaccaaaat
    15181 tccttttaag tcgaatagtg ttatttctgc ttttacatta tcaggacttg aaataataga
    15241 gttaactact tcttcgacct gatttactga caataattta aacatatcat tttgacatga
    15301 tggtgcatag attgttttca gttcatgcat ggttccatta aaatcaaact gtttaagctt
    15361 ataatattca gcaatgccat cttctgctga aactgtataa gtcttagtgt cattgattgt
    15421 tccgctgcct tgtatttttc gaaaaaaagt aaactcattg ccgttagaac ttttctcaat
    15481 ggtgaaaaaa tcattgttta gttctgatgc agtagtccat ttaatttgat gacgattgtt
    15541 tatgcaatta gcatcaaatg aaagtaattc tacaggaagt ggcgtgtttt catttactaa
    15601 tacccaccag tcattggctg tggataaacc ggttacggta acactgcgaa cagcaccgac
    15661 agcgttataa gactgtgctc cctgaaatgg attgtcccaa aaacctccgg cataattata
    15721 atgttgtgct ttgtaagttc cggcaattgc aggaaattca gcaacaggag cagagaatac
    15781 agcattgaat gtggtgctgc ttccagattg gttaattttc caaaagcgat ctacacaatc
    15841 agcgctattg tcggcaccgg tagctgctga gtttacatgt ccaacgccag gaggaaaagg
    15901 aaggttggca ggatttgttt caaatgtaga tgttgacaca aaacctgcat ttccaccagt
    15961 tgatgtaaca gttaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggta
    16081 aacatattca gtatttggag taccaccaaa gttaatttca agaataccat atttctggtt
    16141 gccgccaaca gcattaccca ttccaattcc tgtaacaatc tgattgtcgt caccgaaata
    16201 ttctactgtg ccacttgcag agagtataaa tcccatgttg ccacttgaat tgattgcggc
    16261 agaactaaag ccagaataga acccattagt atgttgaatg cgtattctcg atccgctggc
    16321 accttgtact gtacttttta ggaaatttcc ggtagtataa acagagctat tatttcttaa
    16381 agtgaaagta gcaccagatg acacataaag cattggtaaa ttttgtccgg caaattcttc
    16441 aacatacgat gcaagctgaa catcacctga aatcacttcc aatgttgttc cactcaatat
    16501 cgttataaca gagtggtcaa ttgtatgacc atttagacgg gtaaataatg tagtgcctgt
    16561 acgtgcaaag tcaagggcac cgcgagcagc aattgtacct gcaccgtttc gtttaattgt
    16621 tccggacggg ccaagtaata tattagggct tttaatggtt aagctctgaa cagtaggatt
    16681 caaagtgttg ttaacaaaat tgattgtacc cccggagtgt acaaaatcac cggtgttatc
    16741 atcatcactg ttaatagtaa ctgttgttgt tataggtgga accgttgcat tgtttgtata
    16801 gaaaaaattg ccatttgaca tactaaatcc tcctaaaaca tttatgacga ttggagaata
    16861 agctgaatta cttttcaaac ttaaagtacc acctgaaata atcaagtcgc cattaattgt
    16921 tccagtcagg tcgcccctgc cggcagaaaa attgtttatt ccattattgc aagttaaatt
    16981 tcctgcaata gttatatcca cattgtgtgt agtagtgact actgcacttg gatagttatt
    17041 aaaactattg tttccattat taaaaataag attgctatct aatgtgataa cctcattacc
    17101 ttgtgtaaca cttgaataaa atgttccatt tgctccgtca aaagacatta ccccagaaac
    17161 agttagtttt aaaggtgcag tgttgctata aacaggatgt aaataaggaa tgaaactaac
    17221 taagttggaa acagctgaat aattaacagt aaaatctcca tttacggtgc agattacact
    17281 atcgttggct gcattatatt gtatgtaatt accaccggtg aaattaaatg cacctaaagt
    17341 aaaaatactt ttaccggcag taaaaatatt cggtgcatgg gtgcctttaa agaatcctcc
    17401 tgactgattt aaggttccat caacagaaaa tgtattgcgc cctgttccgt cctgaatacc
    17461 ataaaatgca ccggcattca gattaaagtt gccgccaaca tgaaactcaa gatttccatt
    17521 gttaaggtag gtcccacaaa ctccctgatt ggttgcagta cctgaattca tcagattgaa
    17581 atcaccaaat atatgaatat aggtattgcc ggctcccgaa ttgcctctaa aaaaattact
    17641 tggatttgta gaattaaaca caaaacttct ggtaacagaa attgaatcta ttgcattaga
    17701 acttgcattg gcctgtacat tcatggagcc gccatttagc atcaaatttg aattaatgcc
    17761 aacatttact attccaatgc tgtttgatac aacaaaattt actgtcgaag ctgaggaggt
    17821 taagtcatgg ccaacaatta acattgtcct tgcaacgttt ccgggtgaaa gcataatggt
    17881 tgtattagtc acacccgaaa gattaaaatc gtttaataca tttatagttg cataggatgt
    17941 tggtaaaggg aaaagtggag cgtttgaggc atctaatgca tcatttccac ccaaaaagac
    18001 atttttaagt ccggcagaaa tatttaaatc gcctgtggta taatttaaga atccgtttcc
    18061 ggaatcaaca aatcgaacat aattagggtt ccctgctatt tttgtgttgc ctccaacagt
    18121 taaagtcaat ggtgcattaa cttgcttgca aaaatcaaac ttattacccg aaccttgaat
    18181 aaataagtct cccataatgt tagctatgac actggcactt ctgattggga atgttgaatc
    18241 ataaacggca ataaaatcat gtctcgttat aaaatgacca tttactgtca gatttaatgc
    18301 ccctaaagac atgttcataa tgatggtagg ttgtgttgat aaagtactac tatcagtaaa
    18361 gttgcccatt gttaaggtta ggtttctgtt attaccttcc tgaaaaatta tagcagaatt
    18421 acctctgaga gtaacatcgt ttaaagttaa tgtggttgtg gcaccactcc catcatccat
    18481 ccttagaaca ccatctgtta ctaggagact accttttatt ctgttgggtg aaaactgtcc
    18541 tttttgctcc caggttgcac ctgaaacgga agtaactaaa tttcctatat cactagtaat
    18601 acgtgtaggg cctattaatg gcaatgcagt acttgcccat ttgcgaatta tcagggtact
    18661 tgaaggacta aaactttcaa ctccattttc aaacactttt tcgtcaactg caatattaat
    18721 tcttgggtta tgatcataaa ctgctccgtt acctaaaaca aaatttccgg gtacagtcat
    18781 atcaattctg ccattattaa ttaatgtgcc acttacaacc aaattacctg caaaaaccaa
    18841 ctgagcaaca gcattgttag caatagttaa agtaccaccg gcattaacag ttacagaacc
    18901 taaaatatcg gtattaatcc caataatcgt tactgtggtt cctgtattta taacaactgt
    18961 atcgcctgct gtcggatata atccaggttg attccatatc aatgtattaa aatttccgga
    19021 agccacagat tgaaagatca atgcttcaga acgataagaa ctgaaaacaa agagcacggt
    19081 gaccataaaa aaggaaatga ttttttggaa atctttcatg tttttattga tttaatagtt
    19141 ttggaagaca tttgttaaga tgtcctgtaa gtttaatcgg taaatttact aaaaggttaa
    19201 gagtaaaagc aattttctta ccaatttttt taataactta tgttatcatc aggaaattgg
    19261 taatttgagc acatgacata aatttaaacc ggtaagtata gaataaatat taattgattt
    19321 aaaactttaa tcatataaat ataagttatt gataattaga atattaatag taatattatt
    19381 aaattaaaca tgcaagttag cgttaaataa actttagtct ttttatgaaa tgttggcaat
    19441 cagagaaaga agaaaaatat taacagaaca tgaaatatgt ggtaattata atttaaaatc
    19501 acctatttac cgacaaaatc tttcagatag aaaggttcaa aataggcaag gtcttcaaaa
    19561 gtttgttgcg taaatttttt aaaagcaagc gttactaaac cagttgcaga cattttaaaa
    19621 tcatctaaaa tggtgcaatt tgaatttgtt tttattgatt ccggaagttt ttctgcacca
    19681 tttccaaaaa aacagatctt gttttttaat agatagtcat gttgaaaatg gtcattgatg
    19741 attgcggcat tttccttttc gacataatca agttgaggcg taaaaagtgc tgtaaagact
    19801 tccattctgc gtgcatctat cattgggcat aataacaact gaccgtgaca ttttttgagt
    19861 gcattgtttg ccattgactg taaggtaggg acagaaatta acggcacttt tagagcaaaa
    19921 caaagacctt tagcagttgc tacgccaatg cgcaatccgg tgtaagatcc ggggccgcaa
    19981 ctgataacaa cagcatcaat atttttatag gttaattggt tttgttttaa caattcatca
    20041 atgaaaacag caagtaaaga tgcatgtgat ttgctgttgt gtgtttcttt gcaatcaatg
    20101 attgtttcat tgtcagaaat acaaacggag caagtgtcag tagaagtttc taaatggagt
    20161 agaagagcca ctaaaattat ttttgttctt ctataaaaag ctgattttgt ggaacaactt
    20221 taatcttatc accatcaact agtttttttg atacagcctg ataagggtcg gtgattattt
    20281 cgtcaccaac ttttattcct tcaagaattt caataaaatt attgtcctga atacctgttt
    20341 taacaacatg catttttgca attccattat cattgacaaa gacaatttct ttaacaggtg
    20401 tttcatccgt gttgttagtt gctactacct taacatccgg tgtatcatct gatttcctga
    20461 taacagcttt atcagatgct ctgattgtag tatcacgtgt tgtaactgcc ttaatcggaa
    20521 cggaaacaac gttactaact tttttagttt gtatttcaac tgttgctgac attccaggtc
    20581 taaatggaga atattccgga tggtcagatg gaatcaagtc ggcataagat tcgcgtaaaa
    20641 ttctgatttt aactgcaaag tttgtgacct gctcagttga agtaagggac tcatttggtg
    20701 agttggcaat ttccgttaca atgcctttga attttcgtgg gctgtatgcg tcaatttcaa
    20761 caagtgttgt atcatttagg tgtattctta taatatcgtt ttctccaaca tcggctttta
    20821 cttccatttc ctgtaagtta gaaattctaa gaatttctgt accatctgta aaacttgttc
    20881 cggcaatacg ttcacctttt ttcttgctga ttttagaaat ggtaccatta acaggtgcaa
    20941 aaatttcagt cttatttaaa ttttctttgg cttcacgcac agttgcattt ccactgttga
    21001 tgttgtattg agctcctgac acaccttgtt tggatgcatc tacctcagct tttgcaactt
    21061 cataagcaga ctttatggct tcaaaatcag aagcgctgat taccttttct tcaaatagtt
    21121 ttttgtttct cgtataggcg atttcagctt ttaccaactg actttctgct tgtgaaagtc
    21181 gtgacttgct actttcataa tttgccttag tggtatttag tccggcaaca gctctgtcta
    21241 atgatgaaat gtagatttct ggattgattt tacaaagtaa ctgacctttc tttactacat
    21301 caccttcttt aacaagcatc tcaacaattt cacctgaaac atcagagctg atttttactt
    21361 ctacctctgg ttgtactttt ccactggcag atacagactc tgtgattgtt tgcagtgagg
    21421 ctttttcggt actaacattg gtgatatttt cttcacctaa ccaaccagct tttttagcta
    21481 ttaccaaagc tataataacg actccggcaa tgatgatgat atttcttaat gttttatttt
    21541 tcttactcat ataatttgct taaaatgata gtggttttcc aagataataa tcaagcactt
    21601 ttatttttag aatcaaatcg aattttgatt gaattaaatc tgattgtgat ttggtataat
    21661 tatttctggc atttacgtag tcaatcgaat ttaagagacc ggcattgtac tttttttctg
    21721 tatataaaaa tgccttttcc tgtgctgcta cacttttgtt tgctgcagca tatttgttga
    21781 gtgcagctat tgcatctgca tgagcctgct gaacgttttt atatacagtg ttttttgttt
    21841 gtaatgcatt gaggcgtgca ttttcaaaac ttaattttga tcttttaata ttgctgttaa
    21901 ttgcccatcc gttgaataaa gagatggata agttgaaacc aatggctttc ccgaaattat
    21961 tattcagctg atcttttaaa ggggtatcaa tatatttata attgaaaaca ggttggagta
    22021 cttgctctcc cgatgaggta acagcacctg aaggaacata gccagtgaaa accggactac
    22081 catccaatga tgttgcctga ttggaataat tggtagataa agagccaaat gcagatagct
    22141 ttggatagcg tgcagcacgg gcaatgccta aagattcctt ggcacttttc agcctaagtt
    22201 cagaggcttt tacctccggc agatttttta aagcttcatt gtaaaccgat tctgacgata
    22261 atgaaagaga ttcttgcgat gggatttcca atgtaggagt ttcaataaca atatcactcg
    22321 ctccttcaag ttgcattagt tgaatgagcg tgagtcttga tgaggtgagc agattttctg
    22381 ctgacgcttt attgaattct tctgtggcaa gaagtgcatc tgcatcaagc aaggagcctt
    22441 cggctaacaa atcattatca actaaaattt ttgccctgtt acgttctttt gtaaccgcat
    22501 ctattctgtc gttggttgct tttaattgct cattgctata aagtatctgc aagtaggcag
    22561 ttgcaactga caaggcaata tcattctgca ccttgcgtac atctaattta ctagcatcaa
    22621 aatctatgga gcttttacgc agattatgtt gcagttccag tccctgaaaa attggaagat
    22681 ttccatttaa cgatgcacca cctgactgaa atgtttggtc aacatattga tagcttgtag
    22741 ggtcaaggct tctgccgtag ttgtaattta aagatgaact ggcattgata ctcggaaaaa
    22801 gactgccaac actttgctga taagttgcag ccgcaatttt tgaggcaatg attgattgct
    22861 gaacactaat attgttgttc agtgcatagt caatacagtc tttaagcgac cagattttct
    22921 gggcattaac ttcactgaaa aaaaatatga ataaaaaaat aattatatta tgtttcatgt
    22981 tattccggta actgcgttgc gaaattaaga attgttttat tgagggagct tcaaagtgaa
    23041 attaattgat agaattttat cctcattaac aaactttaac agttaagaat tttgctggat
    23101 tgattaacgg aaggtttatt gttcagatgc gttttttata tcctgaaaac ggttaaactt
    23161 tattattttc gcacactatt ttataaatca tgttcgagaa tttaagtgat aagttagaaa
    23221 aggcgttcaa aaaccttaaa ggtcagggaa agataacaga gattaacgtt gccgaaactt
    23281 taaaagaggt tagaagggca ttgttagatg ctgacgtaaa ttataaagta gcaaaacagt
    23341 ttaccgatac cattaaagaa aaggctttag gacagaatgt attaacggct gtatctcccg
    23401 gtcagttgct ggttaaaatt gctcatgatg aactggcgac attaatgggt ggacagatga
    23461 cggatataca tctgaaagga aatccgtctg ttattctcat gtcggggttg caaggttcgg
    23521 gtaagactac cctgtccggg aaacttgcga attttttaaa gactaaaaaa gggaaaaccc
    23581 ctttgttggt ggcatgcgat gtttacaggc ctgcagccat tgatcagttg aaagttttag
    23641 gtgagcagat aggtgtagaa gtttattctg aaatagagaa taaaaaccct gtggaaattg
    23701 cacgtcatgc aataaagcaa gcaaaacaga atggaaacaa tgtagttgtt gtagatactg
    23761 caggtcgttt ggctatagac cagcagatga tgaatgagat tgctgcattg aaaaatgcaa
    23821 tcgaacctga tgagattttg tttgtagttg acagtatgac cggccaggat gcagtgaata
    23881 cggcaaaagc gtttaatgac agattgaatt ttgacggagt ggttttaaca aagcttgatg
    23941 gtgatactag aggtggtgcg gcattgtcta ttcgttctgt tgtaaataag ccaataaagt
    24001 ttgtgggtac cggtgagaag atggatgcat tggatgtttt ccatccggaa cgtatggccg
    24061 accgtatttt aggtatgggc gacatagtga cgttggtaga aaaagcacag gagcaatttg
    24121 atgaaaaaca agctgccgag ctacagaaga agtttgccaa gaatcagttt aattttaatg
    24181 atttcctttc gcaattgcag cagattaaaa aaatgggaaa cattaaagac cttgtttcga
    24241 tgatacccgg agttggtaaa gctgttaagg atatggacat tgataacaat gcttttaaaa
    24301 gtatagaagc aattattcag tctatgacac cggcagaacg ggccaatccg gatttgatta
    24361 atggaaatag aaaaaaaaga attgcagccg gtagcggaa
//
LOCUS       DAOQNP010000043        24146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_705_length_24146_cov_8.397534, whole genome shotgun sequence.
ACCESSION   DAOQNP010000043 DAOQNP010000000
VERSION     DAOQNP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 24146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24146
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_24146_cov_8.397534"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKY09_07340"
     CDS             <1..763
                     /locus_tag="PKY09_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12709.1"
                     /translation="NVVLHVAYLKKDFNVLESVIIELNQGSKTILHEVRKSPFIKVNE
                     LVGLLRDIEDGWICLLKPGDEWLPYHLSAFRDAMSHYHGFKTYYALNYLSGYSAIRST
                     NPQKNVIGVEHIDLSSQLSLSQLLIHSSVLKNNVFPDFKVDAHFDSLFELIFICSSGI
                     VRINRFTNICRIRKQVHNRSYFDQQTLIVCYLIRNMIKDHQQRFQLLTMNWRMGFIGL
                     LKTLRFFGAGIFFKEMLCYYIGLKRGIRKGQIYVD"
     gene            789..1646
                     /locus_tag="PKY09_07345"
     CDS             789..1646
                     /locus_tag="PKY09_07345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB12710.1"
                     /translation="MPVYNRAELVKDSIASVLSQRFDDFELICIDDGSTDNTLDVLKE
                     IANKDRRIKLISHQNKGRCAARNNGINAASGEWIAFLDSDDYYLENHLSVMYQLIQNF
                     PSFSGIATELVVESKIRDYANCNYFKDQKIFDLNDFAEANPISLNQFCYRKSHYPDLL
                     FPDINILTAEDLLFFRMFSYKHKILKSGIITNFVNSHVNRSVNLISTEQFVYWNKFAT
                     DYFIENYSLPDKINAKLRQNLQLFISNVYLSSNYLKKGLGELIKVMKYPSTYTDIHFY
                     KAIAKIFIN"
     gene            1646..3538
                     /gene="asnB"
                     /locus_tag="PKY09_07350"
     CDS             1646..3538
                     /gene="asnB"
                     /locus_tag="PKY09_07350"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB12711.1"
                     /translation="MCGIVGCFSVSNFFSDQDIRKASSAINHRGPDGDGFFSDSLVQL
                     AHRRLSIIDLDKRASQPMYSSCKRYVMVYNGEVYNYVELRKEIESKFKDNPLSVFKTT
                     SDTEVILRLFEIKGVDFVKDLNGMFAIAIYDTQDNKLFLFRDRFGIKPLYYYHDSSGN
                     FCFASELKAILAIDGIKRKVNNQSVAYFLHTGFVPAPLTIYENIFKLESGNCLIIDRS
                     GLEIHPFVISKQCSQREIIDDEKEIIQSVKNLLIESVKIQLRSDVPYGVFLSGGVDSS
                     LLTAIAAENVNEKLNTFSIGFTEQSHNESEHARVIAKHIGTNHHEFVVYVQDAIDLIE
                     TFFEVYDEPYTDTSGIPTMLVSKLASKHVKVVLSGEGGDELFFGYGTHNWAKRLNTFP
                     FNVFRSPLQKALSCGSSRSKRVAHLLDFDKNSEFLPEHIYSQEQYLFSNKEIAELVSD
                     ELKIEALMSIAQRKKELEKLFSISDSCLPEERQANYELKFPFQDDLLTKVDRATMFHS
                     IEARVPYLDNNLVEYVFSVASDIKIKNGVNKYPLKKILANYVPEELFMRPKKGFSIPL
                     AKWLKSDWKYLLDRYLDKKIIQQAGLVKFDVVQKLIQRFLSGEDYLYNRIWCLIVLHR
                     WYEREQ"
     gene            complement(3542..4303)
                     /locus_tag="PKY09_07355"
     CDS             complement(3542..4303)
                     /locus_tag="PKY09_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12712.1"
                     /translation="MKARYIFILLCLLLSHNIFADTKDDLLFTEANNLYLKQSYFQAI
                     EKYEQLISDNIISFEVYYNLGNAYYKTGNYTKAILNYERAKKIKPDDADVTLNLAIAN
                     QKTIDKIEPTPKVFYNQWWDNYLASSTADTSAVIMLVFLWLFIATIAIMIVIKNASLK
                     KLCFIISLILACCTIFFFITSQTRQSINDSSVEALIISQSAYIKSAPDDKGTNLFQLH
                     EGTKINIVDELGNWKKIKIPNGNVGWVKSKDLETI"
     gene            complement(4307..6178)
                     /locus_tag="PKY09_07360"
     CDS             complement(4307..6178)
                     /locus_tag="PKY09_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNB12713.1"
                     /translation="MVETVLKTKRKPFIAGLALLLMFLTTITTPVFAAEITFIASVNK
                     NPVSTNEQFSITFTLNTQGSNFQAPTFRDFNVLSGPNQSTSMQFINGNMSQSVSYTYY
                     LTANNEGTFRIEPATVNVNGGKVKSNTLTINVVKGQKAQSGSQQQKSNDADAGISSNS
                     VFLRVSVNKSSVYRGEALLATYKLYTKVNLVNYSIDKLPALNGFWSQELKMPQQLELT
                     TENYNGEMYRVGIVKKVLLFPQQSGTLTIEPMEGSCIARIQTQRKRSNNPFDIFNDPF
                     FNDPFFGSAQDVKVNVKSLPVKITVKDLPQENDNAFKGAVGTFNLQATTDNDKVKAND
                     AINLKIKISGKGNLKLIDAPEVEVPTDFEKYDPKVSDNTTISESGVSGSKTFEYLLIP
                     RHEGEYTIDPIKFTYFDLDKKQYVQLHSSEFRVTVKGISGDASAAQSVATSQNKTDIK
                     LLGKDISFIKTGKLITKPATTFYQSGFYYLLIVIPFLSLIILIFYIRKRNKLAGDAEY
                     MRSSRATKMAHKRLSVAKKMIDAKKDDSFYEEISKALYGYASDKLNINFSEMTKENLR
                     QKLIARNIPDALTERIITTIDLCELARYSPQTQNNNKQSIYTETVALITELENHLNK"
     gene            complement(6172..6888)
                     /locus_tag="PKY09_07365"
     CDS             complement(6172..6888)
                     /locus_tag="PKY09_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12714.1"
                     /translation="MKKSNILFLFFISMSFVALAQNEKPLVREGNKEYKNGKFDQAES
                     SYRKAIEKNGSSVEGRYNLGNALYKQNKYDEAAGVYQNLNNEKLSTDVKAKSLYNLGN
                     TLMKSEKYQEGANAYKEALKLSPNDKDALYNYSYALAKLKQQQQQQKQNQDNKKDQKN
                     QQKNQQDKQQQKQDQQKQDQKKEQDQQKQDQKKEQAQQPKISKEDAERMLEALKNDEM
                     NLQKKLAKKEGTRVNIEKQW"
     gene            complement(6875..7927)
                     /locus_tag="PKY09_07370"
     CDS             complement(6875..7927)
                     /locus_tag="PKY09_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB12715.1"
                     /translation="MFRFSHSLILYGLALIPLFIILYISLISWRKKSLKKFGDIHLVR
                     YLTPGKAYTKPLIKFILLMTAFLFLVIGIAGPQVGTKLETVKRKGVDVIIALDVSNSM
                     NAEDIKPSRLERAKQSISRLIDQMQGDRIGLIVFAGEAYTQLPITTDYGAAKLFLSTV
                     NTNIVPTQGTAIGKAIDLAVSSFGNEDSSKKNKAIIVITDGENHEDDAVAAAKEAYKN
                     NITIHTIGMGSINGAPIPVYTNGQRTGFMQDKNGQTVITKIDEHTLAEIAQAGNGKFI
                     RATNSDDGLSIIMKEINAMQKNEFGSKMFTDYADQFQYLLGVALLFLILEFLISEKRS
                     RWMEELNLFGDGDEKK"
     gene            complement(7950..8933)
                     /locus_tag="PKY09_07375"
     CDS             complement(7950..8933)
                     /locus_tag="PKY09_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB12716.1"
                     /translation="MQFAYPQFFWLLLLIPAMLLWQWKVASAKKVRLKLSSDDGFQNY
                     QPSFRQRLVFMPFLFRLLSVIYIIIALARPQSSSQAQQVSTEGISIVLAMDISGSMLA
                     EDFKPNRIEAAKKVAIDFIDHRPNDLIGLVIFSGESFTQCPLTSDHAVLKNLMSKIES
                     GMLTDGTAIGEGLATAVNRLKDAKTKSKVIILLTDGVNNAGAIAPLTAGEIAKTFGIR
                     VYTIGVGTVGMAPYPFKTVFGIQYQNVPVQIDEEICKQISDATDGKYFRATNNKSLKE
                     IYSEIDKLEKTKVEVTEFRRHSEEYFNYALAAGIFFALELLLKYTWLRKLT"
     gene            complement(8954..9919)
                     /locus_tag="PKY09_07380"
     CDS             complement(8954..9919)
                     /locus_tag="PKY09_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12717.1"
                     /translation="MKALSNGFAALLLSILMYTNCVYAQNATAVLQLDTNRIVAGQQI
                     TAKLRFTAPADYQSKWVAIPDTFNGIDVISRSPIDTLPSANKSLITREQKFTLTAFDS
                     GFFVITPFVFNYTKPGDTTNYTVETQAQLLTSNLMPVDTTKAIHDIKGPMNVGITWQE
                     IVMYASVILLLIAIIWYVVYRLRKKKPQIVIKAPEAPKRPAHEIAIEQLEHLQQEKLW
                     QQGDFKMYYTHLSDIMRAYISNRWNFDAMESTTDEIMRSNTALQLSADNFNRLKNTLT
                     LSDLAKFAKYTPLGNENEQAMTDAFIFVNETKVVPTVKPQEQVKS"
     gene            complement(9916..10809)
                     /locus_tag="PKY09_07385"
     CDS             complement(9916..10809)
                     /locus_tag="PKY09_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNB12718.1"
                     /translation="MDATELIKKVRKIEIKTKGLSNQIFSGEYHSAFKGRGMAFSEVR
                     EYIPGDDIRSIDWNVTARLNHPYVKVFEEERELNVMLIVDVSASGNFGTQKQFKRELI
                     AEICAVLSFSAIQNNDKIGLILFSDKIEKFIPPNKGRSHILRIIRELINFEAESKGTS
                     ISAGLQFLSNAIKKKSIAFLISDFYDSAVGQTKSMLEDSLKIANRKHDLVVLKINDLR
                     ESELPDMGLINIYDAETGREQLIDTSSAVVRKNYQHQRQMRNHQINGLLSKSGIDKIE
                     LFTGQNYIQPLMKLFKNRVKK"
     gene            complement(10869..11855)
                     /locus_tag="PKY09_07390"
     CDS             complement(10869..11855)
                     /locus_tag="PKY09_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB12719.1"
                     /translation="MFDIREINEKIQRESEFIDLLQLELSKVIVGQKYMVERLLIGLL
                     SNGHLLLEGVPGLAKTLAIKSLASAIDARFSRIQFTPDLLPADLVGTLMYNQKKEEFA
                     VRKGPIFANFILADEINRAPAKVQSALLEAMQEKQVTIGDETFKLEEPFLVLATQNPI
                     EQEGTYPLPEAQLDRFMLKVVIGYPTREDEKLIIRQNLSQEKTNITPIIRTADILKAR
                     QLVREVYMDEKIEKYIIDIVFATRFPNDNKLGKIAQLINYGGSPRASINLALAAKTYA
                     FIKRRGYVIPEDVRAICHDVMRHRIGLTYEAEAENYTTDMVINEVLNVVEVP"
     gene            11997..12782
                     /gene="truA"
                     /locus_tag="PKY09_07395"
     CDS             11997..12782
                     /gene="truA"
                     /locus_tag="PKY09_07395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNB12720.1"
                     /translation="MQRYFLELFFKGTHYSGWQIQKNAVAVQQKINEALGLLINGSAL
                     KPIETTGCGRTDTGVHATQFFAHFDTPEIEDHKNFLYQLNGILPGDITILDLHVVDKD
                     SHARYDAISRTYQYYIHTYKNGFLEEFSGCYYRRFDVSLMQKAAEILLKHDDFNSFCK
                     SRAQSKTSQCRIANAKWSESKGKLMFEITADRFLRGMVRALVGTMTDVGIGKISLNDF
                     ENIITARNRQKAGHAAPACGLYLTKIVYPFLNVKTNNHLPNHW"
     gene            12926..14266
                     /locus_tag="PKY09_07400"
     CDS             12926..14266
                     /locus_tag="PKY09_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB12721.1"
                     /translation="MAKKQDRKVFVLDTSCILFDHNAITNFKEHNVAIPITVLEEIDR
                     FKKGNDVINFEAREFIRSLDKLSSGASLGDWIPLNGSTKGKFKVLMDESGNGVDANKV
                     FGEKKADHNIINAALALKHETDERNIVLVSKDINLRLKAKSLGLQAEDYMTGKIKDIE
                     GLYTGKSELNNVSKKLIDALYQDGYCKWKDLVKSRPVANHYYILKSKAEGSSALAFYN
                     PITDRIERIHKDHAYGIKPRNAEQAFAMHAVMNPNVLLVTVQGVAGTGKTLIALASAM
                     QQKMNYRQIYLARPIVPLSNKDIGYLPGDIKSKINPYMEPLWDNLKYIQSQYRETDKE
                     YQKISDMVENEKLHITPLAYIRGRSLSNICFIIDEAQNLTPHEVKTIITRAGEGTKII
                     FTGDVFQIDTPYLDAHSNGLSNVIDKMKNSHLYSHITLEKGERSELANLAGELL"
     gene            14337..14927
                     /locus_tag="PKY09_07405"
     CDS             14337..14927
                     /locus_tag="PKY09_07405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNB12722.1"
                     /translation="MIKRCVDLIISFLAIVILLPLLLLIALWVKADSRGSIIYRQMRV
                     GKGGKDFGILKFRTMHPDSDSKGLLTVGGRDPRITNAGFFLRRYKLDELPQLFNVLVG
                     QMSLVGPRPEVRKYVSRYSDEQLKVLNVKPGITDYASIHYSNENEILAQSADPEMTYV
                     QEIMPHKLALNLKYIQEAGLITDLKIIAETVLKIIR"
     gene            15171..17789
                     /locus_tag="PKY09_07410"
     CDS             15171..17789
                     /locus_tag="PKY09_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12723.1"
                     /translation="MDKVLNYYRSVFVVLLSLISINKVHAQMQQMMQKGMNAGRLYGK
                     VMDSKTNKPVEFAAVQVSVFKKDSTGTLKENILNGQLTQGNGDFSLEQLPVMGELTFK
                     IAAMGYKPYEQKVKFEIKMPQGGAQGTNWQQALNAIDKDLGNIKIEPNVVALQEVQID
                     GTAPILELRPDKKVFNVEKNPIATGGTAEDVLKQVPSVNVDMDGNVTLRNAAPQIFVD
                     GRPTTLTVDQIPADAIQEVEIITNPSAKYDASGGMAGILNIVLKKNRRIGYNGMVRAG
                     ADSRGRINLGGDFNVREGKVNVFLSGNLNQRKSKGTGLTERQTLGSTPSNFISQSNEN
                     SSNGYFGFIRSGADIFLDNRNTITVSGSYMNGYFKPLEFLQTKTDTLFPGYTSSSLSD
                     RESSSKREFKNTGGTVAYKRLFPQEGKELTADVNFNSVKSDMGGNYSTQNYLGSGEAF
                     GNPILQKQSIEGENTFVTAQTDMDFPLDKKSKIETGLRGAMRDYSSVNQNFIFDDSQQ
                     QYILVPNQTANYQYIDQVYAAYATYSKSFEKLSLQAGLRAESSFYEGELTQTGQKFST
                     NYPVSLFPSASASYKLNNKTDLQINYSRRINRPNFFQIIPYSDYSDSLNISKGNPGLK
                     PEFTHSIELSAMQTLSRKNTLMASVYYKQTDGVITRYLSYEADTILGRNVLVSTFRNA
                     NTSYAYGIELTAQNNIKGWLDITSNINAYQSVINGKNIESNLKVEQFSWFAKLNLNIK
                     LPKNFSVSLSGDYQSKTALQINNGGGGRGFMGGTQNTAQGFIRPNYGVDAGLRYDFLK
                     DKAASLTLNVSDIFKTRVNDVHSESDFYIQDSWRKRDAQIFRLNFSYRFGKFDTSLFK
                     RKNQKINTEGMQDMSM"
     gene            17854..19071
                     /locus_tag="PKY09_07415"
     CDS             17854..19071
                     /locus_tag="PKY09_07415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB12724.1"
                     /translation="MNVLYISYDGMTDNLGQSQVIPYLKGLTGYGHHFDIISCEKPDK
                     FKAKEAEIRKLLDSLNIGWHPLPYTKRPPVLSSVLDVVRMYRKAYSLHRENKYDAVHC
                     RSYLSAMIGQDMKKKFGTQFIFDMRGFWPDERIDGDLWNIKNPVYRIIYNYFKKKELQ
                     FFNESDLNISLTHAGLNEIKKMSVSSEQINKFSIIPCCSDYDLFKPLPRDIALLRQLG
                     FEESNKILCYLGSIGTWYMFDEMMAMFKQLNIQDNSFRLLIMTPEPPSMVYDSAKKLG
                     INSDFIKVVSATRQQVPQYLSITHLGISFIKPCFSKLSSSPTKMGEMLAAGVPLICNS
                     GVGDVERIINETKTGVVIHGFTEDEMQSVAIKAIELIQIPRENIREQSRSVFALESGV
                     KLYAQAYDKMQKQ"
     gene            19137..20225
                     /locus_tag="PKY09_07420"
     CDS             19137..20225
                     /locus_tag="PKY09_07420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB12725.1"
                     /translation="MSQNKKKILLICPYPEDTAPGQRLRYEQYLNLFRKEGYDIQVSS
                     FMTYRFNNIVYKPGNIPEKIFWTLFGYIKRFLQLFTLWRYDAVYIFLWVTPFGLPLFE
                     KLFVLLNSNMIYDIDDMVFLGHSSKANKFIKILKGTRKITFLMQKAKHIVVSTPSNEA
                     FARKYNNAITDISVAINTDVYLPANKYSNDDVITIGWSGSHSTSRYLFLLEEVLRKFA
                     GQSNIRILVIGDTTFKFNGFDCVTIPWQSATEVKDLQKIDIGLYPLPDEQWVYGKSGG
                     KALQYMALGIPTIATGIGTNFRIIDDGESGFLVRTEEQWIEKIEYLINHPEERKRIGM
                     NGRKKVEKEYSLHVNKKYYLDIFEKVVG"
     gene            20270..21178
                     /locus_tag="PKY09_07425"
     CDS             20270..21178
                     /locus_tag="PKY09_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12726.1"
                     /translation="MKKTIFVLLIACGYFVAKAQFAPIGAQWHYTQCAVTFPYTCGPV
                     EVNAIGTTVINGITCTNLQIAPFTCVGFNYLPVYQSNDTVYRFLYNLNTFTMLYDFNA
                     QPGDIWNIVSNTINQPPPAPLTDTVRIRVDSVNMVNINNHPRKIFYISYADSSHIPFV
                     FEGPIIEGIGSMNFLLTQFNLCDPQITAFRCYNDSTLGLYQPNTLVDCDSVIVVGESE
                     VIKKDYVSLYPNPFTDQICIHSEDFLYRKYDIKLVNIVGKVVYHSKHRQALDQLTIFP
                     KNLAPGTYYLLLESDKKVLQKRLVKL"
     gene            complement(21235..21648)
                     /locus_tag="PKY09_07430"
     CDS             complement(21235..21648)
                     /locus_tag="PKY09_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNB12727.1"
                     /translation="MWWFYAILSALFASLTALFAKLGVANINSNLATGIRTIVVVLMI
                     WIIILTKGETKGIGSLSKQNVFFLIVSGLTTGLSWLFYFKALQLGNLSQVAPIDKLSI
                     ALTIILAVIFLGETMSLKTTIGAALIIAGTLVMII"
     gene            21875..23881
                     /gene="ispG"
                     /locus_tag="PKY09_07435"
     CDS             21875..23881
                     /gene="ispG"
                     /locus_tag="PKY09_07435"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HNB12728.1"
                     /translation="MSNTILKQDTFQLYCNSLTSYQRLKTLEVKIGDVAMGANHPIRV
                     QSMTTTDTMDTKATVAQSIRMIEAGCEYVRITAPSINEARNLQNIKDELRKQGYGTPL
                     IADIHFTPNAAETAARIVEKVRVNPGNYADKKKFDTIDYTDATYAEELERIRERFIPL
                     VKICKEYGTAMRIGTNHGSLSDRIMSRYGDTPLGMVESALEFLRICMDEGYYNIVLSM
                     KASNPQVMVQAYRLLIAKMRAEFMDLNNEQNSLPALFPLHLGVTEAGEAEDGRIKSAS
                     GIGTLLEDGIGDTIRVSLTEDPELEIPVAKKLVSRYNNRASHLSIKPINHQKLPYSPF
                     SYSRRQTKAVLNVGGNHVPVVFADFSGKQNISPASLFPVGYNYSQALDKWHISDQACD
                     FIFAGDQHISFALPGTLSVVYNYKMWLQHSGDERTYPLFKVSELDEIAKCNAAIKFVE
                     LCTEDFFKYQLKIDKLSDAVLVLKTSNEHAMPDLRSVFVGMMQEKSTLPVIIKRVYDN
                     TDLETFLLNSSTDAGALLLDGMGDGVWLCSEKVSPADINQTAFGILQAARTRISKTEY
                     ISCPSCGRTLFDLQETTAKIRAKTHHLKGIKIGIMGCIVNGPGEMADADYGYVGSGAG
                     KITLYKGKEVVRRNIDATLVVDALIELIRDNGDWIEPAVITPSA"
     gene            complement(23865..>24146)
                     /locus_tag="PKY09_07440"
     CDS             complement(23865..>24146)
                     /locus_tag="PKY09_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNB12729.1"
                     /translation="VLKGVYTSISTPDGKCFFNTTGNAGLAKGGSGDTLTGIIAAMLA
                     QGLNATDAAIAAVYIHGLAADCAIKKIHPYSLTASDVTEYLSEAFMQTV"
BASE COUNT         7543 a         4039 c         4589 g         7975 t
ORIGIN      
        1 aaatgttgta ttgcatgttg catatctgaa aaaggatttt aatgtacttg agagcgtgat
       61 tattgaactt aaccaaggta gtaaaacaat tttgcatgaa gtacgaaaat ctccttttat
      121 taaagtgaat gaattggttg gtttgttgag ggatattgaa gatggttgga tttgccttct
      181 gaagcctggt gatgaatggc tgccatatca tttgtcagca tttcgtgatg cgatgagtca
      241 ttatcatgga tttaaaactt actacgcttt aaattactta agtgggtata gtgcaattcg
      301 atcaacaaat ccacagaaaa atgttattgg tgtagaacat atagatttga gtagtcagtt
      361 gagtctttcg caattgttga ttcatagtag tgtgcttaaa aataatgtct ttcctgattt
      421 taaagtggat gcacattttg attcgttatt tgaactgata tttatatgca gttctggcat
      481 agtaagaatt aacaggttta caaatatttg tagaatcagg aagcaagttc ataacaggtc
      541 ttattttgat caacaaacat taatagtttg ttatctgatt agaaacatga taaaggatca
      601 tcagcaaaga tttcaattgt tgactatgaa ttggcgaatg ggtttcatag gattgttaaa
      661 gacattgcga ttttttggtg caggaatatt tttcaaagaa atgctttgtt attatattgg
      721 attgaaaaga ggcattagaa aggggcaaat ttatgttgac tgaaaagaag cctttctttt
      781 ctgtaattat gcctgtatat aatcgtgcag aacttgtgaa agattcaata gcgtctgtac
      841 taagtcaaag atttgatgat tttgaactga tatgtattga tgatggctca actgataata
      901 cattagatgt tttaaaagag attgccaata aagatagaag gatcaaattg atttctcatc
      961 aaaataaagg tcgttgcgca gccaggaata atgggataaa tgctgcttca ggtgaatgga
     1021 ttgcatttct tgattcagat gattattacc ttgaaaatca tctttcggta atgtatcaat
     1081 taattcaaaa tttcccttca ttttccggca tagcaacaga attggtagtg gaaagcaaaa
     1141 tcagagatta tgctaattgt aattatttta aagatcaaaa aatatttgat ttaaatgatt
     1201 ttgcagaagc taaccctatt tcactaaatc agttttgtta taggaaatca cattatcccg
     1261 atttactttt tcctgatata aatattctga ctgcagaaga tttattgttt ttccgtatgt
     1321 ttagttataa gcataaaatt ttaaaatcag gcattattac aaattttgta aactcacatg
     1381 tcaacagatc tgtaaatctg atttccacag aacagtttgt ttattggaat aaatttgcaa
     1441 ctgattattt tattgaaaat tactctttgc cagataaaat caatgctaag ttgcgacaaa
     1501 atttgcagct ctttattagt aatgtttatt tgtcttcaaa ttatttaaaa aaaggacttg
     1561 gtgaattaat taaggttatg aaatatcctt cgacttatac tgatatccat ttttataaag
     1621 caattgcaaa aatatttatt aactgatgtg cggaatagta ggttgttttt ctgtaagtaa
     1681 ttttttttct gatcaggata tcaggaaagc atcttctgct attaatcatc gtgggccaga
     1741 cggtgatgga tttttcagtg atagtttagt tcaattggca catcgtagat tatccatcat
     1801 agatcttgac aagagagcgt cacaacctat gtattcttca tgtaagcgtt atgtgatggt
     1861 ctataatgga gaagtctata attatgttga gctgcgaaaa gagatagaat ctaaatttaa
     1921 ggacaatcca ttgtctgttt ttaaaactac aagtgacacg gaagttattt taagactgtt
     1981 tgagataaaa ggtgttgatt ttgttaaaga tctgaatggc atgtttgcta ttgctattta
     2041 tgatactcaa gacaataaat tgtttttatt cagagataga tttgggataa aacctttgta
     2101 ttactatcat gattcatcag gtaatttctg ttttgcatcc gaacttaaag caatacttgc
     2161 aattgatggt ataaaaagaa aggtaaataa tcaatctgta gcttattttc ttcacacagg
     2221 atttgttcct gcgccattaa caatatatga gaatatattt aaactcgaaa gtggaaattg
     2281 cctgataatt gacagaagtg gtttggagat tcatccgttt gtgataagta agcagtgctc
     2341 acaacgcgaa attattgatg atgaaaagga aataattcaa agcgttaaaa atcttctgat
     2401 agaatcagta aagattcaat tacgcagcga tgttccatat ggcgtttttt taagcggagg
     2461 tgttgattca agtttgctta cagcaatagc agcagaaaat gtaaatgaaa agttaaacac
     2521 attttcaatt gggtttacgg aacaaagtca taacgagtct gaacatgcaa gagtcattgc
     2581 aaaacacatt gggacaaatc atcatgaatt tgtagtttat gtacaagatg ctattgatct
     2641 gattgaaaca ttttttgaag tttatgatga gccttatact gatacttcag gtattcccac
     2701 aatgttagtt tctaaacttg cctcaaagca tgtaaaggta gtgttgtcag gtgagggagg
     2761 tgatgagttg ttttttggtt atggtacgca taattgggca aagcgattaa atacttttcc
     2821 ctttaatgta ttcaggagcc ctctccaaaa agcactttct tgtggtagtt cacgcagtaa
     2881 gcgtgttgca catttgttgg attttgataa gaattctgaa tttttacctg aacatattta
     2941 ttcacaggaa caatatttat tcagtaataa agaaatagca gaattggttt ctgatgaatt
     3001 aaagatagag gctttgatga gtatcgcaca aagaaagaaa gaattagaaa agttgtttag
     3061 tattagtgat agctgcttgc ctgaagaaag acaggcaaat tatgagttaa aattcccttt
     3121 tcaggatgat ttgttaacca aggttgaccg tgctactatg ttccactcta ttgaagcacg
     3181 cgtaccttat ttggacaata atctggttga atatgttttc tcagttgcca gcgatattaa
     3241 aatcaagaat ggcgttaata aatatccatt aaaaaaaatt cttgccaact atgtgccaga
     3301 agaattattt atgcgaccta aaaaaggatt ttcaatacct ttagcaaaat ggctaaaatc
     3361 tgattggaaa tatttgcttg atcgctatct tgataaaaag ataattcaac aggcaggatt
     3421 agtaaaattt gatgtggtac agaaattaat tcagcgtttt ctttccggag aagattattt
     3481 atataatcgt atctggtgtt taattgtact tcatcgctgg tatgagcgtg aacaatagtg
     3541 attaaattgt ttccaaatct ttagacttaa cccatccaac atttccattg gggattttta
     3601 tttttttcca attacccaat tcatcgacaa tattaatttt agttccttca tgtaattgaa
     3661 acaggttggt gcctttatca tcaggagcgc ttttaatata ggcactttga ctgataatta
     3721 aagcctctac tgatgagtcg tttatacttt ggcgtgtttg tgatgtgata aagaaaaata
     3781 ttgtacagca tgccagaatt aaactgatga taaaacataa ttttttcagt gaagcatttt
     3841 taattactat catgatggct attgttgcta taaaaagcca aagaaaaact aacataatga
     3901 cagcagatgt gtctgcagtg gaggaggcaa ggtaattgtc ccaccactga ttgtagaata
     3961 cttttggagt tggctcaatt ttgtcaattg ttttttgatt ggcaatagct aagttcaggg
     4021 ttacatcagc atcgtcaggt ttaatttttt ttgctctttc gtaatttaaa attgcttttg
     4081 tgtaattgcc tgttttataa tatgcgtttc ccagattgta ataaacttca aatgaaatta
     4141 tgttgtctga aattaattgt tcatattttt caatagcctg gaagtagctt tgttttaaat
     4201 acagattgtt ggcttcagtg aataataaat catcttttgt gtcagcaaaa atattgtgcg
     4261 aaagcaaaag acaaagtagt ataaatatat atcttgcttt catagtttat ttgtttaaat
     4321 gattttcaag ttccgtaatg agcgcaacag tttcagtgta tatactttgt ttgttgttgt
     4381 tttgcgtttg tggcgaatat cgagcaagtt cacacaagtc aattgttgta atgattcttt
     4441 cagttaatgc atccggtata tttctggcga ttaatttctg tcttagattt tcttttgtca
     4501 tctcagaaaa attaatgtta agtttgtcag aagcatatcc atataaggct tttgatattt
     4561 cttcgtaaaa agagtcatct ttcttggcgt caatcatttt ttttgcaaca gacaatcgtt
     4621 tatgagccat tttcgtagct ctactgctgc gcatatattc tgcatcaccg gcaagtttat
     4681 tccttttcct gatataaaat atcagtataa tcaatgatag aaatggaatt actatcaata
     4741 aataataaaa accactttga taaaatgttg ttgcaggttt ggttattaat tttccggttt
     4801 taatgaatga aatatcttta ccgagtagct taatgtctgt tttattttgt gatgtcgcta
     4861 ctgattgtgc agcacttgca tctcccgaaa tacctttaac tgtaactcta aactccgatg
     4921 aatgtaactg tacatactgc tttttatcaa ggtcgaagta ggtgaatttt attgggtcaa
     4981 tagtatattc accttcatga cgtggtatca ataaatattc aaaagtttta cttcctgaaa
     5041 caccgctttc tgaaatggtg gtgttgtcac tgactttcgg atcgtatttt tcaaaatctg
     5101 ttggcacttc aacttccggt gcatcaatta gttttaaatt tcctttgccg gatattttta
     5161 ttttaagatt aatagcatca ttagctttaa ctttatcatt atctgttgta gcctgaagat
     5221 taaaagttcc aactgcacct ttaaatgcat tgtcattttc ctgaggaaga tctttgactg
     5281 ttatttttac cggtaagctt tttacattaa cttttacatc ttgtgctgat ccgaaaaacg
     5341 ggtcattaaa aaatggatcg ttaaaaatgt caaatgggtt attggagcgt ttacgttgtg
     5401 tttgaatacg tgcaatgcaa ctgccttcca taggctcaat agtcaatgtg cctgattgtt
     5461 gtggaaacaa caatactttt ttaactatac caactctgta catttcacca ttgtaatttt
     5521 cagtggttag ttctaattgc tgcggcattt ttaattcctg cgaccagaat ccatttagtg
     5581 caggcaattt atcaatgctg tagttaacca aattgacttt agtgtatagt ttataagttg
     5641 ctaacaaagc ttctcctcga taaacagatg atttgtttac actaactctt aaaaagacac
     5701 tgttactact aatacctgca tcagcatcat tacttttttg ctgttgcgaa cctgattgtg
     5761 ctttttgacc tttaactaca ttaattgtaa gcgtattgga ttttactttt cctccattga
     5821 catttaccgt agcaggttca attcgaaaag tcccctcatt gttggctgtg agataatagg
     5881 tgtatgaaac ggactgcgac atgttcccgt ttataaattg catgcttgtg ctttgattag
     5941 gaccgcttaa tacattgaaa tctctgaaag ttggtgcctg aaaattgctg ccctgtgtgt
     6001 ttaaagtaaa ggttatactg aattgctcat tggtgctaac agggttcttg tttacggaag
     6061 ctataaaagt tatttctgca gcaaaaacag gagttgttat tgttgttaaa aacatcaata
     6121 aaagtgctaa gcctgcaata aatggctttc gttttgtttt caaaactgtt tctaccattg
     6181 cttttcaata ttaactcttg tgccttcttt cttagctaat ttcttctgca aattcatttc
     6241 atcatttttt agtgcttcaa gcatacgttc tgcatcttct ttagaaattt tcggttgctg
     6301 tgcttgttcc tttttctgat cttgcttttg ttgatcttgt tcttttttct gatcctgctt
     6361 ttgttgatct tgtttttgtt gctgcttatc ctgttgattt ttctgttggt tcttttgatc
     6421 ttttttgtta tcctgatttt gtttttgctg ctgctgttgc tgctttagtt tggctaaagc
     6481 ataagaatag ttatataagg catctttgtc attaggacta agttttaaag cttctttata
     6541 agcatttgcc ccttcctgat atttttcaga tttcattaat gtattaccaa gattgtagag
     6601 cgattttgct tttacatcag tagaaagttt ttcgttgttc agattttgat atactccggc
     6661 agcttcatca tatttatttt gtttataaag tgcattgcct aaattgtaac gaccttctac
     6721 actactgccg tttttttcta ttgcttttcg atagcttgat tctgcctgat caaatttgcc
     6781 attcttatat tctttattac cctcacgcac caatggtttt tcattctgtg caagagctac
     6841 aaaactcatg gaaataaaaa ataagaaaag aatattactt tttttcatca ccatcgccaa
     6901 ataaatttaa ttcttccatc catctgcttc gtttttctga aatcagaaac tctaagataa
     6961 gaaataataa tgctacaccc aaaagatatt gaaattggtc tgcatagtct gtaaacatct
     7021 tactgccaaa ttcgtttttt tgcatagcat tgatttcttt cattataata ctaagcccat
     7081 catcactatt tgttgctctg ataaatttac cgtttccggc ttgtgcaatt tcagctaatg
     7141 tgtgctcatc aatttttgta attacagtct gcccattctt gtcctgcata aaacctgtgc
     7201 gctgcccatt agtgtaaacc ggaataggag caccattaat ggagcccatt ccaatggtat
     7261 gaatggtgat gttgttttta taagcttctt tggctgcagc aactgcatca tcctcatgat
     7321 tttctccatc agtaatcaca atgattgctt tgtttttttt cgaagaatct tcattgccga
     7381 aagatgaaac agctaagtca atagcctttc caatggcagt tccctgtgtt ggaacaatgt
     7441 tggtattaac tgttgaaaga aacaattttg cagcaccata gtctgtggtg ataggcagtt
     7501 gtgtataagc ttcacctgca aagacgatta acccgattct gtcaccttgc atttgatcaa
     7561 ttaaccgtga aattgattgc ttggcacgct ccaggcgact tggtttaatg tcctcggcat
     7621 tcatactgtt actcacatca agtgcaatga tgacatcaac acctttgcgt ttaactgttt
     7681 ctaatttggt tcctacctgc ggacctgcaa ttccaattac cagaaacaga aaagccgtca
     7741 ttagtaaaat aaattttata agtggtttgg tatatgcttt cccgggagtg aggtatctga
     7801 ctaaatgtat atcaccaaat ttcttcagtg atttttttcg ccagctgatt aagcttatat
     7861 atagaatgat aaatagcggt atcaaagcca gaccatataa aataagacta tgtgagaatc
     7921 ggaacatttt tacgcttttg ctttttcttt tatgtaagtt ttcttaacca ggtgtatttt
     7981 aacagtagtt ctaatgcaaa aaatattcct gccgcaagtg cataattaaa atactcttca
     8041 ctgtggcgtc tgaactcagt tacttcaact ttcgtttttt ccagtttatc aatttcagaa
     8101 tatatttctt ttaaagattt attgtttgtt gcacggaaat actttccatc cgttgcatca
     8161 gaaatttgtt tacagatttc ctcatcaatc tgcacaggta cattctgata ttgaattcca
     8221 aatactgtct taaatgggta gggtgccata ccaacagtgc cgacaccgat ggtataaact
     8281 ctaatgccaa aagttttggc tatttcaccg gctgttaatg gtgcaattgc tccggcatta
     8341 tttacaccgt ctgtaagaag aataattact ttactttttg tcttagcatc tttcagacga
     8401 ttcacagctg ttgccaatcc ttctccaata gcagtgccat cggtcaacat accactttca
     8461 attttcgaca tcaggttttt taatactgca tggtctgaag ttaacgggca ctgagtgaaa
     8521 ctttctccgc taaaaattac caaaccaatt aaatcattgg gacggtgatc aataaagtca
     8581 atggcaactt tttttgcagc ttctattcgg ttgggtttaa aatcttctgc aagcatactt
     8641 cctgatatgt ccattgccaa tacaattgat atgccttctg ttgaaacctg ctgtgcctgt
     8701 gatgatgatt gtgggcgtgc aagcgcaata ataatgtaga taacacttag tagtcggaat
     8761 aaaaacggca tgaacactaa gcgttgtctg aagcttggtt gataattctg aaaaccatca
     8821 tcagaagaaa gtttaagtct gactttttta gcagaagcaa ctttccattg ccacaacagc
     8881 attgccggaa tcagcaacag caaccagaaa aactgaggat aggcaaattg tatgttatca
     8941 aataaactca tgcttatgac ttaacctgtt cttgtggttt cacggttgga acaaccttgg
     9001 tttcgtttac aaatatgaaa gcatctgtca ttgcttgttc attttcattg cctaatggtg
     9061 tgtattttgc aaattttgcc agatcactta gcgttaatgt gtttttcaat ctgttaaaat
     9121 tatcagcact taattgtaag gcggtgttac tacgcattat ctcatcagtt gtactttcca
     9181 tagcatcaaa attccagcgg ttgctgatgt atgctctcat tatgtccgaa agatgcgtgt
     9241 aatacatctt aaaatcgcct tgttgccata atttttcttg ctgaagatgc tccaattgtt
     9301 cgatagcaat ttcatgagca ggccgttttg gagcctcagg tgcttttata actatctgtg
     9361 gttttttctt tctcaggcga taaacaacat accagattat tgcaattaat aaaagtatta
     9421 ctgaggcata cattactatt tcttgccatg ttattccaac attcattggc cctttaatat
     9481 catgaatggc tttggtggtg tcaacaggca tcagatttga tgtcagcaat tgtgcttgtg
     9541 tttctacggt atagtttgtg gtatcacccg gttttgtata attgaataca aaaggagtta
     9601 tgacaaaaaa acctgaatca aaagcagtaa gtgtgaattt ttgttcacgt gtaatcaggc
     9661 ttttgttggc ggaaggtaag gtgtcaattg gtgagcgtga aataacatca atgccattaa
     9721 atgtatccgg aatggccacc catttagatt gataatctgc aggtgcagta aaacggagct
     9781 tagctgttat ctgttgtcct gcaacaattc tgttggtgtc aagttgaaga acagcagttg
     9841 cattctgtgc atatacacaa tttgtgtaca ttagtatgct taaaagcagc gcagcaaatc
     9901 cgttacttaa tgctttcatt ttttaaccct gttcttaaaa agtttcatca atggctgaat
     9961 gtaattctgc cctgtaaata attctatttt gtcaatgcca ctcttgctta acaatccatt
    10021 gatttgatga ttacgcattt gacgttgatg ctgataattc tttctgacta cagcacttga
    10081 ggtgtcaata agttgttctc gaccggtttc tgcatcataa atatttatca aacccatgtc
    10141 aggtaattct gattcgcgca agtcgtttat ctttaatact actaaatcat gttttcgatt
    10201 tgcaattttt aatgaatctt caagcatgct tttggtttga ccaacagcac tatcataaaa
    10261 gtcagaaatt aaaaatgcaa tactcttttt ctttatagca ttacttagaa actgaagccc
    10321 ggcacttatt gatgtgcctt tactttcagc ttcaaagttg attaattcac ggatgatacg
    10381 taagatgtga gagcgacctt tgtttggcgg aataaatttt tcaattttat cgctaaataa
    10441 aatcaaacct attttatcgt tgttttgtat tgcagagaaa gaaagtacag cacagatttc
    10501 tgctatcaac tcacgcttaa attgtttttg agtaccaaaa tttcctgatg cgcttacatc
    10561 tacaatcaac atcacattta attcgcgttc ttcttcaaaa acttttacat aggggtgatt
    10621 tagacgtgca gtaacattcc agtcaataga gcggatatca tcacctggaa tatattccct
    10681 cacctcactg aaagccatac cacgaccttt aaaagccgaa tggtattcgc ccgaaaaaat
    10741 ctggtttgaa agtccttttg ttttaatctc aatcttccgg acttttttta tcagttctgt
    10801 tgcatccact gccttggcta ttgtttgttg attctattga aataaattta ttgaaaggtt
    10861 taaaattatt aaggcacttc aacaacgtta agtacttcat taatcaccat gtcagttgta
    10921 tagttttctg cttctgcctc ataagttaaa ccaatacggt ggcgcattac atcatgacat
    10981 atagcacgca catcttcagg aataacatag cctctgcgtt ttataaaggc ataagtttta
    11041 gctgcaagag caaggttgat acttgcacgt ggagatcctc cgtagttgat gagttgtgca
    11101 atctttccta gtttgttatc attcggaaaa cgtgtggcaa aaacaatatc aataatatat
    11161 ttttcaattt tttcatccat ataaacttcg cgaaccaatt gtcttgcttt aagaatatct
    11221 gcagtgcgga taataggtgt gatgtttgtt ttttcctgtg ataaattctg acggataatc
    11281 agtttctcat cctctcttgt tggatatccg ataacaactt ttaacatgaa gcgatctaac
    11341 tgtgcttccg gtaggggata agttccttcc tgctcaatag gattctgagt tgcaagtact
    11401 aaaaaaggct cctcaagttt aaatgtctca tcaccaatag tgacttgctt ttcctgcatt
    11461 gcttcaagca aggcactttg cacttttgca ggggcacggt ttatttcatc agctaatata
    11521 aagtttgcaa aaatgggtcc tttgcgcaca gcaaactctt cttttttctg attgtacatt
    11581 aatgttccta ctaaatcggc aggtaataaa tcaggagtaa actgaatgcg gctgaaacgt
    11641 gcatcaattg cagatgcaag tgatttgatg gctaatgtct ttgcaagacc gggaacccct
    11701 tcgagtaata agtgaccatt ggagagtaag ccgatgagta gtctttctac catgtatttc
    11761 tgcccaacaa ttaccttact cagttctaat tgcaacaggt ctatgaattc actttcgcgt
    11821 tggatttttt catttatttc tctgatgtca aacataaata ttgatgatta tttgcgatgc
    11881 aagtttaaaa gtttagctgt gaaaagaatg tgaaatggac tgttaatttg tgttaagtaa
    11941 tagaaacggc aaatatacta ttcagtacat aatcagataa cttatttttg tacgctatgc
    12001 aacgatactt tcttgaatta ttttttaaag gtacgcatta tagtggctgg caaattcaga
    12061 agaatgcagt tgctgtgcag cagaaaatta acgaagcatt gggtctgcta atcaatggtt
    12121 ctgcactgaa accaattgaa actacaggct gtggccgtac agatactggt gttcatgcta
    12181 cacagttttt tgcacatttt gatactcctg aaattgaaga tcataaaaat tttctttatc
    12241 agctgaatgg aattttgcct ggtgatatca ctatattaga cctgcatgtt gttgataaag
    12301 attctcatgc ccgatatgat gcaataagtc gtacttatca atactatatc cacacttata
    12361 aaaatggctt tttagaggag ttttctggat gttattaccg gaggtttgat gtttcactca
    12421 tgcagaaggc ggctgaaata cttttaaaac atgatgactt taatagtttt tgcaaatcac
    12481 gtgctcagag taagacaagt cagtgtagaa ttgctaatgc aaaatggtca gaaagtaaag
    12541 ggaaattaat gtttgaaatt actgccgaca gatttttaag aggtatggtg cgtgcattgg
    12601 ttggaacaat gactgatgta ggaattggaa agatcagcct caacgacttt gaaaatatta
    12661 ttactgcacg caacagacaa aaagcaggac atgcagcacc tgcatgcggg ttgtatctta
    12721 caaagattgt gtatccattt cttaatgtta aaaccaacaa tcatttgcca aatcattggt
    12781 aagtttcaat tcctatgact gagataaaat aagtcgtctg gagtcagtct gttattaaca
    12841 agtctttgtg taatttaagc aatgaattaa gttttcacct tatagtgccg gtcatatatt
    12901 tgtcatagac caaacaagtt ttagcatggc aaaaaaacaa gatcgcaaag tattcgtact
    12961 cgatacctct tgcatccttt tcgaccacaa cgcaataact aactttaaag aacacaatgt
    13021 ggccatcccc ataacggtac tcgaagagat agatcgtttt aaaaagggaa atgacgtaat
    13081 caatttcgaa gctcgcgagt ttatcagatc actcgataag ttaagttcgg gtgcatcact
    13141 tggcgactgg attccgctca atggctctac caaaggaaag tttaaagtac tgatggatga
    13201 gagtggaaat ggtgtagatg ccaataaagt tttcggtgag aaaaaagctg atcataacat
    13261 aattaacgca gctctggcat tgaagcatga aaccgatgag cgaaacattg ttcttgtcag
    13321 taaagatatc aatctgcgac tgaaagctaa atcacttggc ctgcaggcag aggattatat
    13381 gaccggaaaa attaaagata ttgaaggcct ttacaccggt aaatcagagt tgaacaatgt
    13441 ttcaaaaaag ttaattgatg cattgtatca agatggctat tgtaagtgga aggatttggt
    13501 aaaatcacgt cctgttgcca atcattatta tattctcaaa agtaaggcag aaggttcttc
    13561 tgcattggcg ttttacaatc ctataacaga tagaatagaa cgtattcata aagatcatgc
    13621 ttatggtata aaacctcgca atgccgagca ggcttttgct atgcatgcag tcatgaatcc
    13681 gaatgttttg ctggtgactg ttcagggtgt agccggtaca ggcaaaacat taattgcatt
    13741 agcatcggct atgcaacaaa aaatgaatta ccgtcagata tatcttgcac gtccaatagt
    13801 tccactcagc aataaagaca ttggttacct gccgggtgac ataaaatcaa aaattaatcc
    13861 ctacatggag ccattgtggg ataacctgaa atacatccaa agccagtatc gcgaaacaga
    13921 caaagaatat cagaaaattt ctgatatggt ggagaatgag aaattacaca tcacaccttt
    13981 ggcgtatatc cgcggacgta gcttaagtaa tatttgtttc attattgacg aagctcagaa
    14041 tctgacacct catgaggtaa aaactatcat tacccgtgct ggcgaaggaa ctaaaataat
    14101 tttcacaggt gatgtgtttc agatagatac accatatctt gatgcacata gtaatggcct
    14161 gagcaatgta attgataaga tgaaaaattc gcacctctat tctcatatca cattggaaaa
    14221 aggtgaacgc agtgagttgg caaaccttgc cggagaattg ctttaaaagg ttaaccggtt
    14281 ttttcgaata taatttaatt cccgtcaggt agcgttacag cggttatctt tgcatcatga
    14341 tcaaacggtg tgttgattta attatttcgt ttttagcaat tgtgatactc ttgccattgt
    14401 tactgctcat tgcactatgg gttaaggccg atagcagggg tagtattatt tacagacaaa
    14461 tgagagttgg caagggagga aaagattttg gtattcttaa attcaggacg atgcatcctg
    14521 attccgacag taagggatta ctcactgtag gtggtcgcga cccaagaata acaaacgcag
    14581 gattttttct gcgtaggtat aaattagatg agctacccca attgtttaat gtacttgtag
    14641 gccagatgag tcttgttggt ccaagaccgg aagtgcgtaa atacgtgtca cgttattcag
    14701 atgagcaact aaaggtgttg aatgttaaac ccggaattac cgactatgca agcattcatt
    14761 acagcaatga gaatgaaatt ttagctcagt cagcagaccc tgaaatgacc tatgtgcagg
    14821 aaattatgcc acataaatta gcactcaatc taaagtatat acaagaagca ggtttgataa
    14881 ccgacctgaa aattattgca gaaacagttc tgaaaattat caggtaggta gcatgtttga
    14941 ggacttttaa tgttttttta ttcttgtgtt aatggttgtt aaattttctc tagtttgagc
    15001 gaaaataaat gattttaaaa tgagttttca ttctgtaaaa taagttttaa atcccttcaa
    15061 actttaaaga gcaaaaaagt gttattgttg caattaaatt aagttttata gaattaaagt
    15121 tttttctaaa tacttttaat ttgccattga tttattttaa gaagaaaaag atggataaag
    15181 ttttgaatta ttatagaagc gtgtttgttg ttttgttgag tttgataagt ataaacaaag
    15241 tacatgcaca aatgcaacag atgatgcaga agggaatgaa tgcgggccgt ctgtatggta
    15301 aggttatgga ttcaaaaacc aataaacctg ttgaatttgc tgcagtgcag gtttctgttt
    15361 ttaaaaagga cagcacaggc acattaaaag aaaatattct taacggacaa ctaacacagg
    15421 gcaatggaga ttttagcttg gagcaattac ctgtaatggg tgaactgaca tttaaaattg
    15481 ctgcaatggg ttacaaacct tacgagcaaa aagtaaagtt tgaaataaaa atgcctcaag
    15541 gcggtgctca aggaactaac tggcagcagg ctctcaatgc tatagataaa gatttaggta
    15601 acataaagat tgaacctaat gtggttgcac ttcaggaagt tcagattgat ggcactgctc
    15661 cgattttaga attacgcccc gacaagaaag tttttaatgt tgaaaaaaat ccaatcgcaa
    15721 caggaggcac ggcagaagat gtgctgaaac aagtgcccag tgtgaatgtg gatatggatg
    15781 gaaatgtaac tttacgtaat gctgcaccac agatatttgt tgatggtcgc ccaacaactt
    15841 taaccgtaga tcagattcct gcagatgcta tacaggaagt agaaattatt acaaacccat
    15901 cagcaaagta cgatgccagc ggaggcatgg ctggtatatt aaacattgta ttgaaaaaaa
    15961 acagacgtat tggttataac ggaatggttc gtgccggtgc agattcaaga ggaagaataa
    16021 atttaggagg cgattttaat gtgcgcgagg gaaaagtgaa cgtgttttta agtggtaacc
    16081 taaaccaacg taaatcaaaa gggacaggac taaccgagag gcaaacactt ggttcaactc
    16141 ccagtaattt tataagccag agcaatgaaa actccagtaa tggctacttt ggatttattc
    16201 gttcgggagc tgatattttt ttagataaca gaaataccat aactgtttcg ggtagttata
    16261 tgaatggata ttttaaacca ttagagttcc tgcaaacgaa aacagatact ttatttccgg
    16321 gttacacatc atcttcgttg tcagacagag agtcttcaag caagcgcgag tttaaaaata
    16381 cagggggcac agttgcctac aaacgtcttt tcccacaaga aggtaaagaa cttactgcag
    16441 atgtaaattt caactctgta aaaagtgata tgggagggaa ctacagtaca caaaattatt
    16501 taggtagtgg cgaagctttc ggcaatccca ttctgcaaaa gcaaagtatt gaaggtgaaa
    16561 acacatttgt tacagcacaa accgatatgg atttcccact tgataaaaaa tcaaaaattg
    16621 aaacaggttt acgtggtgcc atgcgcgatt acagcagtgt taatcagaat tttatttttg
    16681 atgattcgca gcaacaatat attttagtac ctaaccaaac agcaaattat cagtatatag
    16741 atcaggttta tgcagcttat gcaacctaca gtaagtcatt cgaaaaatta tcgctgcagg
    16801 caggactaag agcagaaagc tcgttctatg aaggtgagct tacacaaacg ggtcagaagt
    16861 ttagtacaaa ttatcctgtt agtttgtttc ccagtgcatc ggctagctat aaactgaata
    16921 acaagacaga tttacagata aactattcac gtagaataaa caggcctaac ttttttcaga
    16981 tcattcctta ttctgattat tccgattctt taaacatcag caaaggaaac ccaggattga
    17041 aacctgagtt tacacattca atagaattgt cagccatgca aactttgagt cgtaagaata
    17101 cactcatggc atcagtttat tataaacaga cggatggtgt tattacacgt tatctgagct
    17161 atgaagctga tactatattg ggtcgaaatg tgttggtaag cactttccgt aatgccaaca
    17221 caagctatgc ttatggtata gaattgactg cacaaaataa tattaagggc tggctcgaca
    17281 ttacttcaaa tattaatgct tatcagagtg taattaatgg aaagaatatt gagtcaaatc
    17341 taaaagttga acagtttagc tggtttgcca aactcaatct aaacattaaa ttaccaaaga
    17401 atttttctgt tagtctttcc ggtgattatc aatctaaaac agctttgcaa ataaataatg
    17461 gtggcggagg acgagggttt atgggtggaa cacaaaatac agcacaaggc tttataaggc
    17521 caaattatgg tgttgatgca gggttgcgtt atgatttcct gaaagacaaa gctgcatcgc
    17581 ttacactaaa cgtttcagac attttcaaga caagagttaa tgatgtacat tcagaatctg
    17641 atttttatat acaggatagc tggcggaagc gtgatgcaca gattttcaga cttaacttca
    17701 gttatcgttt tggaaaattc gacacttcac ttttcaaaag aaaaaatcag aaaataaata
    17761 ctgaaggcat gcaagatatg agcatgtaat gtttattctt tcgtttgtca atacatctga
    17821 attcgcttca aatgcaatat ttttggacgg tttatgaatg tactttatat ttcctatgat
    17881 ggaatgacag ataaccttgg gcagtcacag gtaattcctt acctgaaagg actcaccgga
    17941 tatggtcatc attttgatat tatcagttgt gaaaagcccg ataagtttaa agctaaagaa
    18001 gcagaaataa gaaagttact tgattcttta aacataggat ggcacccatt gccctataca
    18061 aaaagaccac ctgtgttatc atctgtttta gatgttgtca gaatgtacag aaaagcatac
    18121 agtctgcaca gagaaaataa gtatgatgca gtgcattgtc gaagttatct ctctgccatg
    18181 ataggacagg atatgaaaaa gaagtttggc acccaattta tttttgacat gcgcggcttc
    18241 tggcctgatg aacgcattga cggtgattta tggaatatta aaaatcctgt ttatagaatc
    18301 atttataact atttcaagaa aaaagaactg cagtttttca atgagtcaga cttgaatatc
    18361 agtctcacac atgcagggtt gaatgaaatt aaaaaaatga gtgtcagtag cgaacaaata
    18421 aataagtttt ctataattcc atgctgttct gactatgatt tgttcaagcc cttaccaaga
    18481 gatattgcac tgctcaggca acttggtttt gaagaatcaa acaagatatt atgttatctc
    18541 ggttcaatag gaacatggta tatgtttgat gagatgatgg ctatgtttaa acagttgaac
    18601 attcaggata attcattccg cttgttaatt atgacacctg aaccaccatc aatggtttat
    18661 gattcagcaa aaaaattggg aataaattca gattttatca aggtggtgag cgcaacacga
    18721 cagcaggtgc cacaatatct ttcaattact catctgggaa tatcatttat aaaaccttgt
    18781 ttttcaaaat taagcagttc cccaaccaag atgggggaaa tgcttgctgc aggtgttcca
    18841 ttgatttgta atagtggtgt aggagatgtg gagcgcatca ttaatgaaac aaagacaggt
    18901 gtagttattc atggttttac agaagatgaa atgcaatctg ttgcaataaa ggcaattgaa
    18961 ctgattcaga ttccaagaga aaatatcaga gaacaatctc gctctgtatt tgcattggaa
    19021 agtggagtta aactatatgc gcaggcgtat gacaaaatgc aaaaacaata attacaaaat
    19081 catctttatt tttgcagaca atttgctgac gttgttttta cagacaacaa taatttatga
    19141 gccaaaataa aaagaaaatt ctgctgattt gcccttatcc tgaagataca gcaccgggac
    19201 aacgtttgcg ctatgaacaa tatctgaact tgttcagaaa ggaaggatat gatattcagg
    19261 tgtcatcttt catgacttat cgttttaaca acattgttta taagcccgga aatattcccg
    19321 aaaagatttt ttggactttg tttggctaca tcaaacgatt tttgcaatta tttacccttt
    19381 ggcgctatga tgccgtgtat atttttttat gggtaacgcc ctttggattg ccgctgttcg
    19441 aaaaactttt tgtactcctc aattcaaata tgatttacga tattgatgac atggtttttc
    19501 ttggacattc ttctaaagcc aataagttta ttaaaatatt gaaaggcaca agaaaaatta
    19561 cattcctgat gcagaaggct aaacacattg ttgtttcaac accttctaat gaggcgtttg
    19621 caaggaaata taacaatgca attactgata tttcagtagc catcaataca gatgtttatc
    19681 ttcctgcaaa taaatacagt aatgatgatg ttataacaat aggatggagc ggcagccata
    19741 gcacaagtcg ctatttgttt ctgttggaag aagtactgcg gaagtttgcc gggcaaagta
    19801 atatcagaat cctggtaatt ggagacacaa cgtttaaatt taatggcttt gattgtgtaa
    19861 ccattccctg gcaatcagca acagaagtta aagatcttca gaaaattgat atcggtctct
    19921 atccactacc tgatgaacaa tgggtttatg gtaagagtgg gggcaaggca ttacagtata
    19981 tggctctggg gattccaacc attgcaacag gaattggaac caacttcaga ataattgatg
    20041 atggtgagtc agggttttta gtaagaacag aagagcagtg gattgaaaaa attgaatacc
    20101 tgataaatca tcctgaagag agaaaacgca ttggaatgaa tggaagaaag aaggttgaaa
    20161 aagaatattc actacatgta aacaaaaaat actatctcga tatttttgag aaggtggtag
    20221 gttaaatatt tcttgtttgt catgatgttt taagtatctt agcactgcaa tgaaaaagac
    20281 aatttttgta ttgcttatag cttgtggtta ttttgtagct aaagcacagt ttgcacctat
    20341 aggtgcacag tggcactaca cacaatgtgc agttaccttt ccttatacat gtggccctgt
    20401 tgaagtaaat gcaataggaa ccactgtaat aaatggtatt acttgtacca atcttcagat
    20461 tgcccctttt acttgtgttg ggtttaatta tttgccagtt tatcagagta acgatactgt
    20521 ttatcgtttt ctctacaatc tcaacacatt taccatgctt tatgatttta atgcacaacc
    20581 cggtgatata tggaacatcg tttcaaacac cattaatcaa ccaccgcccg caccacttac
    20641 agacacagtg cgtattcgtg ttgactcagt aaatatggtt aacatcaaca atcatccccg
    20701 caaaatattt tacatttctt atgccgattc atcacacatt ccatttgtat ttgaagggcc
    20761 tattattgaa ggtattggca gtatgaattt tttgcttaca caattcaatt tatgcgatcc
    20821 tcagattact gctttcagat gttataacga ttcaacatta ggattatatc agcccaatac
    20881 cttggttgac tgcgactctg taattgttgt tggtgaaagt gaagtaatca aaaaggacta
    20941 tgtgagttta tatccaaatc cattcaccga tcagatatgc attcactcag aagatttttt
    21001 gtacaggaaa tacgatatta aactggtgaa tattgttggc aaagttgtct atcacagcaa
    21061 gcataggcag gcacttgatc agctaaccat ttttccaaaa aatcttgctc ctggcactta
    21121 ttatctgctg ctggaatcag acaaaaaagt gctgcaaaag cggttggtga agttgtaaat
    21181 tccccgttca atattatctt gaatgtttga aggttaaaag aagaggaaaa aatactaaat
    21241 aatcatcacc aaggttcctg caataattaa agctgcacct atggtagttt tcaacgacat
    21301 agtttcgcct aagaaaatta cagctaaaat gatggttagt gcaatgctta gtttgtcaat
    21361 tggtgccacc tgagataaat tgcccaactg caatgctttg aaataaaata accaagaaag
    21421 acctgttgtt aagcctgaaa cgattagaaa aaaaacattt tgcttagata acgaaccgat
    21481 tccttttgtt tcaccctttg tcagaataat aatccaaatc attaacacta ctactattgt
    21541 cctgattcct gtagcaaggt ttgaattgat atttgccaca cctaatttcg caaataatgc
    21601 cgttagtgag gcaaacaagg cagagagtat tgcataaaac caccacatag tttcaaaatt
    21661 tagacgattg aaatcattga ataaaagttt attgatttat tattgatttt gtgaaatgta
    21721 aaaaatgaaa ttggggttat tataaaactc gttcacacaa gtctgactat tttctgcaca
    21781 ctaaaatatt aaaaattgaa tttcaaaaat aaggtttact ttgtactgct gagcaagcaa
    21841 aataccgata tttgtatttt tattatttat aaacatgagt aacaccattc ttaagcaaga
    21901 cacttttcag ttgtattgca acagccttac ttcctatcaa agactaaaaa cgcttgaggt
    21961 aaagattggc gatgttgcca tgggagctaa tcaccccatt cgtgttcaaa gtatgactac
    22021 taccgatacc atggacacaa aggcaacagt tgcacaaagc atcagaatga tagaagccgg
    22081 ttgtgagtat gtacgaatca ctgcaccaag tatcaacgaa gcacgtaacc tgcaaaacat
    22141 aaaagacgaa ttgcgaaaac agggttatgg tactcccttg attgccgata ttcattttac
    22201 acccaatgca gcagaaacag cagctcggat tgttgagaag gtaagggtga atcccggtaa
    22261 ctatgccgac aaaaaaaagt ttgataccat tgattatacc gatgcaactt atgctgaaga
    22321 gttagagcgt attcgtgagc ggtttattcc attagtaaag atttgtaagg agtatggtac
    22381 tgctatgcgt attggtacca atcatggttc tttgagcgac cgtattatga gtcgctatgg
    22441 tgatacacca ttaggcatgg tggagtcagc acttgagttt ttgcggattt gtatggatga
    22501 aggttattat aacattgtgc tttctatgaa agcaagtaat cctcaggtca tggttcaggc
    22561 atatagactt ttgattgcta aaatgcgtgc agagtttatg gatttgaata atgaacaaaa
    22621 ctcactgcct gctctttttc ctttgcatct tggcgtgacg gaagcaggtg aggctgaaga
    22681 tggacgaatt aaatccgcat caggtatcgg cacattactc gaagatggta ttggtgatac
    22741 gataagagtt tccttaacag aagatccgga gcttgaaatt cctgttgcaa aaaaattggt
    22801 atcgcgctat aataatcgtg cttcgcacct atcaataaaa ccaattaatc atcagaaatt
    22861 gccttattct ccatttagtt acagcagacg acaaacaaaa gctgttttga atgttggagg
    22921 caatcatgtt ccggttgtgt ttgcagattt ttcaggcaaa caaaatatct ctcctgcatc
    22981 tttatttcct gtgggctata attattctca ggcattggat aaatggcata tcagcgatca
    23041 ggcatgtgat tttatttttg caggtgatca gcatatttca tttgcattgc ccggaacgct
    23101 tagtgtcgtt tacaactata aaatgtggct acaacattcc ggtgatgaaa gaacatatcc
    23161 attgtttaaa gtgagtgagt tggatgaaat tgcaaaatgc aatgcagcca taaagtttgt
    23221 tgaattatgc actgaagatt tttttaaata tcaattaaag attgataaac tctctgatgc
    23281 tgttctggtc ttaaaaacat caaacgaaca tgcaatgccg gatttgcgta gtgtgtttgt
    23341 tggtatgatg caggagaaaa gtactttgcc tgtcatcatt aagcgtgttt atgataatac
    23401 tgatttagaa acatttcttt taaatagctc caccgatgcc ggtgctttgc ttttggatgg
    23461 tatgggtgat ggtgtttggc tttgttcaga gaaagttagt cctgcagata ttaatcaaac
    23521 agcatttgga attttacagg ctgcacgaac tcgcatttca aaaacagaat atatcagttg
    23581 cccgtcatgt ggccgcacct tattcgactt acaggaaact acagctaaaa ttcgtgcaaa
    23641 aacacatcac ctcaaaggca ttaagattgg cataatgggg tgtattgtaa atggtcccgg
    23701 tgaaatggcc gatgcagact atggctatgt aggttccggt gctggaaaaa taacacttta
    23761 taaaggcaaa gaagttgttc gcagaaatat tgatgcaaca cttgttgttg atgcactcat
    23821 agaattgatt cgcgataatg gtgactggat tgagcctgct gttattacac cgtctgcata
    23881 aaagcttcag aaagatattc tgttacatca cttgctgtta aagagtaggg gtgaattttt
    23941 ttaatagcac aatctgctgc caggccatga atataaactg ctgcaattgc agcatcggtt
    24001 gcgtttaagc cctgagcaag cattgcagca attatgcctg ttagtgtatc gccactgcca
    24061 ccttttgcca gtcctgcatt tcctgttgtg ttgaagaaac attttccgtc aggagtagaa
    24121 atggaagtgt aaacaccttt taagac
//
LOCUS       DAOQNP010000044        23879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_719_length_23879_cov_8.048313, whole genome shotgun sequence.
ACCESSION   DAOQNP010000044 DAOQNP010000000
VERSION     DAOQNP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23879
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_719_length_23879_cov_8.048313"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3968
                     /locus_tag="PKY09_07445"
     CDS             <1..3968
                     /locus_tag="PKY09_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNB12730.1"
                     /translation="VILSNIHFTYRDFKWDDKTPCIDFEDIDVSDFNATLYNIKPTDS
                     SFSFYGSRISLKEKSGFKINSFSTYAAFYNDSMSFNKILIRTPYSSINAGQYSMSYNS
                     MTDFEDYINKVIMKGEFNESTISSNDIQYFAEELFGLDKFVEFSGTAKGTVSNLKTKK
                     LKIEYGKNTRFEGDVSLNGLPDVNTTFINLNIKHLSTNKADIETIKQYPFTEQQYIKL
                     PDNISELGKIVYRGVFTGFYNDFVSYGNITTAIGNLSTDVNLKFKNKADESTYSGKLT
                     ADNFDIGKFWSLGNAVGTISLNAQLNGTGFTSEKVNARLTGLVNHVNLNGYDYSNISL
                     DADVARKLFNGTINMNDRNAQVAFKGSIDFREKLPLFDFNAVVKNAALTKLNLIKRDT
                     SALLSTEANLNMRGFNFDEFEGKLQLKNISYSEEFKSAFVEKILVKSGMEDNNRNLNV
                     TSDLLDLNINGQYILSKIVYSFGHVLNCHLPPFMLKLSPDMEKTNTVFSFSGKIKDVN
                     PILNIFTPELAVSNQTVFSGRLNRQHCEIEMDATSPWINYKNFENKNIQLTANNKNSL
                     LNIDLSATQVKISDSLILKNILLDAETQKSKSSIVLTVANDSLSTSKVRLATEQTYTN
                     GATRFHLLESLIQIKGEQWQVDPSNEILFDSASIAFDKVLFTNKSQSLMLQGKVSHNS
                     DDVLSLNLFKFKIPLLNDLLAVYDIKAGGAASGRLNFSSLYQKAIITGEMRVDSFAFN
                     DEPLGNANVNFDWLTDQKKLHIEGAIEQNSRNSAVVKGDYIFKPKNDELSFNIKVDKL
                     SITPLERYLKGFASKIVGLATGELNLTGLAKAPNLTGKARLQRASILIDYLNTRYSVL
                     PEQDIVFDEKYIAFNNIGISDDQNHKGSVDGKIFHNHFSDFTFSLRVNANKLQCLNTN
                     AFQNELFYGKAFATGYAKIIGTLNLLKLDVVARSESGTQIFIPLSNPEEVTQSNYITF
                     INVNDTAAKKFIITNKVDLTGIQMDLQLEATPEAEIQLIFDSKVGDVMRGNGSGNLRM
                     LISQAGEFSMYGDYNILAGDYLFTLQNIVNKRFKIQSGQIQWAGSPYDATININAKYN
                     LRASTFDLIPDSSNRSRVPVEVLLQLKNNLMSPDVVFDVNVPNATPAVRTSLSSILGN
                     EREMNRQVFSLLVLNRFSPPEGKNTSEESGSGNAVGQNLSEFVSQQLSNWASQLSDKF
                     NIGVNYRPGDALNRDEFDVSIATELFRNRVAVESNVGVANNSNQTSSIIGDFQVEFKI
                     SKDGNLRAKAFNKTNTNNLINNLNSQYTQGIGVFYRKEFNTVSDLVESFRKRKKTTSP
                     N"
     gene            complement(3965..5161)
                     /locus_tag="PKY09_07450"
     CDS             complement(3965..5161)
                     /locus_tag="PKY09_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12731.1"
                     /translation="MIKEIRDKYLREFKEETYSDFLAYFDNLYNRIVEFRIAESPVFI
                     PKKLKDLLIEAGNDILTQSLAKDYLIKSEQAIPYGLKVPNEHNHPHFIAIDFAICKDD
                     SGSYLPQLIELQGFASLFFWQDLLPRTYEKYFQIPDGFSHFFNGWNTEKLRALMRKVV
                     LGNHKPENVVLLEIEPEKQKTWIDFWETKTHTGVEPVCISKVIKEGRKLFYELNGVKT
                     PIYRIYNRVIFDELLQRKDLIKDFNLTDNVDVEWAGHPNWFFRISKYTMPFIKSRFAS
                     ECYFLSDVDISKIDLTDFVLKPLFSFAGSGVLIEVTTSDIMNIPAEKRNEYLLQRKVN
                     YVPCMETPDGAAKVEVRLLYIWDDAETKPQLVLNLTRLSKGAMIGVSHNKNKTWVGGS
                     SSLMEI"
     gene            complement(5224..5628)
                     /locus_tag="PKY09_07455"
     CDS             complement(5224..5628)
                     /locus_tag="PKY09_07455"
                     /inference="COORDINATES: protein motif:HMM:NF023145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agenet domain-containing protein"
                     /protein_id="HNB12732.1"
                     /translation="MKKLIITCLAFIGFITFTYAQKVNEKVQIKSNGAWYPGKILKIN
                     ADDHTYYVSYDGWDEGWNEWVPIDRLKDFGKEAPAQPLTKFKVGDRVEVEYGMVPEPA
                     TVIEVGENKYHIKYDKTVFKDKWVTEREIKKL"
     gene            5776..7032
                     /locus_tag="PKY09_07460"
     CDS             5776..7032
                     /locus_tag="PKY09_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB12733.1"
                     /translation="MVQDKRALFFNYLFWLTGFLLPFDQPWINYALGLLLLFFILQWL
                     TGKYQIKIQNPLLWLPLSYFLLHCIGMIYTQSQYAGWIEISAKSSFLIFPLLFSSNAI
                     SREMIRTFKLFFVFGSGIICLYNLLLSFNQYLLHGNMNSFYYVAFSKGMHVQYLTIYL
                     NLAILFILEILFNTSSQTNKIKKIFWVLLVLFFLINITLLASRLATVVSYVTILFYTV
                     VRIISSPNYIRYSVLAALFAASVLIIDYFAFKKINRFEQVEIFLNEKTNFVDFSKTEY
                     NSTTLRIPLWINAYEVIQRNPLFGVGTGDVKSSLDSMYLKNKFTYAHQNHFNPHNQYL
                     QTGVAFGLTGISLLMLMMLIPLYYAIRNRHYIFIAFQCIFLLNMLTESILERQAGIIL
                     FCLFYAIFSSEYVAATIKTVPEQNNT"
     gene            7055..8269
                     /locus_tag="PKY09_07465"
     CDS             7055..8269
                     /locus_tag="PKY09_07465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNB12734.1"
                     /translation="MKVPFAPPRMDKRVVDEVADTLLSGWITTGPKTKKFEKLLAAYC
                     NVPNILALNSATAGLEIMLRWFGVKEGDEVILPAYTYSATANVVVHCGAKPVFVDVGA
                     DFNILPSEIEKAINKNTKVIMPVDFGGLPCDFQKIHSIVQSENIKNKFVSATDVQRQL
                     GRILILSDAAHSFGATVNNIKTGALTDVSVFSFHAVKNLTTAEGGAIALNLPNPFDNQ
                     SIYDYLCIYTLHGQNKDALAKMQKGNWRYDIIEAGFKCNMTDIMASIGLVELERYADT
                     LERRKQIVSNYNTAFEKYPWAELPEFISDWRESSYHLYPIRIKNCTEKQRDAIMQKMF
                     DADIAVNVHFIPVPMFSFYTKLGYSLQHYPKAKDNYEREISLPLFYNLTDEQQQFVID
                     NLIKAVNETLAG"
     gene            complement(8446..11820)
                     /locus_tag="PKY09_07470"
     CDS             complement(8446..11820)
                     /locus_tag="PKY09_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNB12735.1"
                     /translation="MQKKTIVATLFIALFAIQGNAEWVKITSDLPSKSAVVVSGTPGN
                     NVMEISISGFEKHRVKSLAGEYYSITSPEMTPRMEQGTPDLPKLALSLIIPDDGDMSL
                     NIEPIEELTFQNIKIAPSKGNLYRDIKPSDVPYSFGDIYSQNVYFPANNGFLRSPFIL
                     RDFRGQTVVLNPFSYNAVTQTLKVISKIKVTMQHKSGSGTNLLNRTSSLQNVDRDFAS
                     IYQNVFRNAGSYINYLPVSEDGEMLIISDASFMPAMQPFIDWKVKRGLQVSMVDAATA
                     GSTVQQIKTFIQNYYNTHNLKYVLLVGDAQQIPTPVLSGGASDPSYGYLLGNDSYAEV
                     FVGRFSAQTVTDVITQVQRTVEYEMLPTPGVNWYSKGICIGSDQGPGDDNEMDFQHQQ
                     VIRGKELAFTYTDVAELYDGSQGGNDMSGNPSASMLSSEINNGASIITYTGHGSSASL
                     GTTGFSNANATALTNTGMLPFIWSVACVNGEFANATCLAESFLRAQYNGQPSGAVATF
                     MSSINQSWNPPMDAQDEMVDLLVGTNSSNIKRTFGGLSMNGCMHMNDQYGAAGYEMTD
                     TWHCFGDPSLNVRTATPYTITASHSPVLFPGASQLAITCNTDSALACLSSNGQILATG
                     LVIGGNVILNFNTITSLDTLYLTITAFNGIPYMVAIPVVATTGPFVSLTSNVINDAGG
                     NANQLPDYNEIVDVDLMLENFGQGDAVGVGLTVTTTCPYVTINSSSLNKVNIQSGNAA
                     GIINAFNYTVAAFVPDNTPAVFDIILTDSVGTVWNTSITHLLKAPVLQVYSITVSDPT
                     GNNNGVLEPGETADVTIKTNNKGHSATTTSTATLATSSPYLTINNTSYNTGVVQALSD
                     VDATFNVSLASNIQIGSTFDMQFNIDANPYSDNKIFNMVAGEILENFEYGNFTTYPWL
                     NNGNHPWTITNSGTFQGAYAARSAIIADGDTSDLAITVNVYNNDSISFYMLISSEMGW
                     DFLNFYIDGTLIDSYSGVYSWAYKSYPITIGQHTLTWAYIKDEVCCIGGQDLAMLDNI
                     QFPPSTMVTSVKEIYSGNSIAVYPNPVQDELTIITKDLKNAEVKLFTTDGKLVYVASN
                     IISDVYKMPLNNISQGMYYLQVKADNKLYNTKIIKR"
     gene            12001..12633
                     /locus_tag="PKY09_07475"
     CDS             12001..12633
                     /locus_tag="PKY09_07475"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12736.1"
                     /translation="MAVYRFKVSFEDFEDIYREIEVTSEQTFADFYKAILSSIKFEDR
                     GTASFFMSNDSWKKGVEISNSDKTDRDGKPVAKTDKSFLKSYIADPHQKIYFLIDCES
                     VWAFHIELVKILAAADVKLSYPHVRKVSGDAPSQFKVKIAPVLEDDDFVDTDVSDESN
                     SVADEFAGENDYDEDDTATLEGEEGEERTDEDSEEFNDVEMGSDENYEES"
     gene            12694..13695
                     /locus_tag="PKY09_07480"
     CDS             12694..13695
                     /locus_tag="PKY09_07480"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HNB12737.1"
                     /translation="MKKGDKVSFKDEKLNGVILEILPAGSVLVELEDGFTMEALQTQL
                     VVTVPFIKQQIQTDVKEALSEQNTSTHNIPFDLNNNIIEFYTIPSKADRVLTGSVDYF
                     LTNGTPNLLLLIFGKRSNNNHTILHTGVLEPNSYVCLSTFKRDALINIESFYIQILIA
                     ADGEYKRPFTKDISVELPELNQTKNTAPGILSSSKKTSLINLNVADEIQFKDLLSKFD
                     NSQSARTSDNKKNRKQQPVSDSDISKEIDLHIEKLTADFQNMTNSEMLALQISTFRKE
                     MDNAIVKDCKQLIFIHGIGDGVLRNAIMNETKNYADIKVFMANHAKYGAGALEIRFL"
     gene            complement(13690..15435)
                     /locus_tag="PKY09_07485"
     CDS             complement(13690..15435)
                     /locus_tag="PKY09_07485"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HNB12738.1"
                     /translation="MMHYRFSISHAASHFVDIELTIQTNGDETLLKLPVWRPGRYEEG
                     NFAKNIRNFKVQDISGKEVKWKKVSKSSWLLQTNKRDTIKINYQYYCNQLDAGACFVN
                     DEQIYINPIHCCFYTDDNRGNACTVEIAYNFSKVATSLTPVEKNIFSAKNYDELVDSP
                     FIASDALKHYGYTVADCRFNVWIQGNYKPDIEKLIKDFKSFTEVQLNMMKSIPVKDYH
                     FLIQAVPHAFYHGVEHLASTVLVIGPSANLNDSVLYNELLGVASHELFHVWNIKTIRP
                     VEMQPYNYDSPNYAETGFVYEGATTYYGDLFLARCGYFSEEEYFNELNIRINKHLSNE
                     GRFNYSVAQSSFDTWLDGYVTGIPGRKTSIYDEGSLVSLILDFLIRKYSDGKNSLDDV
                     FVELYNAFGKKKIGYTSADYLRIASEKAGRSLETFFNDIVYNACSYQSLLEEVLLFAG
                     LALEEIPSKKYAEGYFGFKTNDSAAIPEVMNVATGSPAFENGLAVGDLIIGVNGWKAD
                     KNYNVHINQIEGHQTSFLISRGSRIKTIEMMPTVKNYFSTWKVKHISEKNSAQEQFFN
                     QWAKNLRTHYKQRSL"
     gene            complement(15437..17515)
                     /locus_tag="PKY09_07490"
     CDS             complement(15437..17515)
                     /locus_tag="PKY09_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB12739.1"
                     /translation="MIAEKKLESTPISTEIVLKAFRLMCTARAMSDLYEENATFTARY
                     VHACSRGHEAVQIALGLQLKNIDYLSAYYRDDSILLSIGMTPYELMLQLMAKRDDPFS
                     GGRTYYSHPSLRRDDMPKIPHQSSATGMQAIPTTGIALGMHYLENQKLVEYADGEYPV
                     TVCSLGDACVTEGEVSEAFQMAVLKNLPILYFVQDNKWDISAHASEIRTMNAAEYAKG
                     FKGLETRSIDGTDFFQSYNAIKEVLDIIRTERRPFLIHAKVPLLGHHTSGVRREWYRH
                     DLEEAKKKDPLPKFIQQLEEAGFTSDELDAIKKETRTLVEDDFAKAKAADDPLPEDLF
                     KHDYAPTTITEEKGIRQPAGKEPTVMVDCALFAVKELLQKHPECLFYGQDVGARLGGV
                     FREAATLAKTFGDDRVFNTPIQEAFIIGSTVGMSAVGCKPIVEVQFADYIWPGLNQLF
                     TEVSRSCYLSNGKWPVSCVIRVPIGAYGSGGPYHSSSVETVVLNIRGVKVAYPSTGAD
                     LKGLMKAAYYDPNPVVIFEHKGLYWSKIKGTEEARTIEPDEDYIIPFGKARIVQQAEA
                     LHDENTLTVITYGMGVYWAKAAAQEFKGRIEIIDLRTLNPLDETTIFESVRKHHRCLV
                     LTEEPIKNSFAEALSGRIQQQCFTALDAPVVAMGSENMPAIPLNSTLEFTMIPNAQKV
                     AEQMSYLLNY"
     gene            complement(17539..18303)
                     /locus_tag="PKY09_07495"
     CDS             complement(17539..18303)
                     /locus_tag="PKY09_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNB12740.1"
                     /translation="MTEGTVESIIKEQVAEITFFHPQSNSLPGVLLRKLATTIEEAGA
                     NESAKVIVLRSKGEKAFCAGASFDELISINSEAQGKEFFSGFASVINAIRKAPKFVIA
                     RVQGKAVGGGVGLACSADYTLAHVSASVKLSELAVGIGPFVVGPAVERKVGTGAFTQL
                     TVNATDWQSAQWAKEKGMFASLHDNIEELDNAVNKLAQQLAASNPEAMKMLKQIFWKG
                     TESWDTLLMERAGMSGKLVMSDFTRNAIEKFKTKTS"
     gene            18548..21052
                     /locus_tag="PKY09_07500"
     CDS             18548..21052
                     /locus_tag="PKY09_07500"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNB12741.1"
                     /translation="MQFNIPAHLTGLTDKEVELSRLKFGINQKKTEAGNSIFSWIVDL
                     FKEPMLILLLAVSVIYLIIGDHGEAIFMFLAIVAVSAISFYQDNRSKKALEALEKLNE
                     PLSTVIRNAQVIKVPTHELVVGDLCIVEEGKLINADGKIVHSNDFSVNESSLTGESLS
                     VYKNSESKDNLVYDGTVAVSGLAVFVVEQIGKETRLGKIGVSITEIKIESSPLQIQIK
                     KFVMTMAIFGSIIFLLVCIVNYLNTKNLIESLLSGLTLAMSILPEEIPVAFTTFMALG
                     AWKLMRDGVIIKRSNIVETLGSTTVICTDKTGTITENTMHLKHLFLLKTNKTYDESEF
                     DTTDLSTLIDYAMWSSEPLPFDPMEKTLHHVYMQTQKTDERTNYKMIHEYPLAGKPPM
                     MTHIFENANKQRIIAAKGAPEAIINVSVLSEGEKENILNVIHDFGNKGYRVLGVAQSN
                     FKGYNFPEKQQELKFTFLGLVVFYDPPKQGIQTVFQHIYDAGIKVKVITGDNTETTKT
                     IAKQAGILNVAEAIEGKDVVNLNEKQLRQEVEEKTLFTRMYPEAKLSVVHALKKKGEV
                     VAMLGDGVNDGPALKAAHIGVAMGNKGTEIAKAAASLVITNDDLDKLIVGIAAGRRIY
                     SNLKKAIQYIISIHIPIILTVTLPLFLDWIFPHIFTPVHVIFLELVMGPTCSIVYENE
                     PLEKNIMKQKPRIMTETFLSWKELGISIFQGLAITLGVLFIYQWSVWLGGNEEKTRAL
                     VFTTLIFANVFLSLVNRSFYYSILACFKHKNILFPIVIGATLIMLFLILYIPAFADFF
                     KLAGLNLREIIMAIIVAAISVMWMEIYKWTKRRKFS"
     gene            21189..22172
                     /locus_tag="PKY09_07505"
     CDS             21189..22172
                     /locus_tag="PKY09_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNB12742.1"
                     /translation="MNKFIKIIVYSLGILLLIVIGFLTYVKVALPDVGPPPDIKIVAT
                     AEKIERGSYLAHHVTLCMDCHSTRDWSYFSGPMVAGTEDKGGETFDQKFGFPGKYIAP
                     NITPFHLKDWTDGEIFRAVTSGVSKDGRALFPIMPHLLYGQLDKNDIESIIAFIRTLK
                     PIENNTEVSSSDFPMNFIINTIPKKPSFTTIPSQHDKLNYGKYIATASGCMECHTKQV
                     KGKIVGQPYAGGFEFMLPGNTVVRSANITPDKETGIGTWTNQQFIERFKMYSDSVYAN
                     VKVAPGTFQTTMPWTMYAGMHQEDLSALFVYLQSLPPINNAVEKFTAANGK"
     gene            22188..22730
                     /locus_tag="PKY09_07510"
     CDS             22188..22730
                     /locus_tag="PKY09_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNB12743.1"
                     /translation="MTTFISILRGINVSGHRLIKMDALKELCASLKMQQVKTYIQSGN
                     IIFQSTLHNCDAISKKLESAILKKFGFEVPVITLSQSELKSCLQDNPFIKEKKLDAAY
                     FHVTFLSEQPTNNDSIETEAIKLRNDRFVIKGRCIYLYCPDGYGNTKLTNSFFENKLK
                     VTATTRNWKTVNELLRLAEN"
     gene            complement(22797..>23879)
                     /locus_tag="PKY09_07515"
     CDS             complement(22797..>23879)
                     /locus_tag="PKY09_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12744.1"
                     /translation="EKERKDSLAKAKAEEERLAREQAKEQSRLAALEKARQDSIAKAD
                     AAAKAAEEARLAALEKERKDSLAKAKAEEERLAREQAKEQARLAALEKARQDSIAKVE
                     AAAKAAEDARLAALEKARQDSIAKADAAAKAAETARLAALEKARQDSIAKADAAAKVA
                     EAARLAALEKARKDSIAKAEADAREQEKLRLAALEKARQDSIAQAKVLADKKAKEEAA
                     ERARLAALEKIRQDSLTKAQALADAEAKRKAQEEADAQRLREIEAQKQALARMNEQND
                     KKQQNTATLPVFEDKDYAEGLTEETIDESNRKIYRTIIKKSNTTDVFSKVVYNWGGVY
                     FFKNTTSMSESNYNNELKKYKDQLGK"
BASE COUNT         7513 a         4527 c         4166 g         7673 t
ORIGIN      
        1 gagttatttt aagcaatata cattttacct atcgcgattt taaatgggat gataaaacac
       61 cttgcattga ttttgaagat attgatgtgt cggactttaa tgctacgctt tacaacatca
      121 agccaacgga cagctcattt tcattttatg gcagtagaat atctctgaaa gaaaaatcag
      181 gatttaaaat aaactcattt tctacttatg cagcatttta taacgacagc atgtcgttta
      241 ataaaattct gatacgcaca ccctactctt ctattaatgc cgggcaatac agtatgtcat
      301 acaatagcat gacagatttt gaagactaca tcaataaggt gataatgaaa ggtgagttta
      361 atgaaagtac gatttcttca aacgatatcc aatactttgc tgaggaactt ttcggcttag
      421 acaaatttgt tgagtttagc ggtactgcaa aaggaacagt cagtaatctt aaaacaaaaa
      481 aactaaagat tgaatatggg aagaacacac gatttgaagg tgatgtcagc cttaatggtt
      541 tgcctgatgt gaatactact tttatcaatc ttaatataaa acacctttca accaacaagg
      601 ccgatattga aaccatcaaa caatatccgt ttactgaaca acaatacata aaattgcccg
      661 ataatatttc tgaacttggc aagattgttt ataggggtgt ttttacagga ttttacaacg
      721 actttgtttc ttatggtaat atcacaacag caattggcaa tctgtcaacc gatgtaaatt
      781 taaaatttaa gaacaaagct gatgagtcaa cttattccgg taaactaaca gccgataatt
      841 ttgatatcgg taaattctgg agcttgggaa atgctgtagg aacaatcagc ttgaatgcac
      901 aactcaatgg cactggattt acatctgaga aagttaatgc acgactgacg ggtttggtaa
      961 atcatgtcaa cctaaatggc tatgattatt ctaacatcag cttagatgcc gatgttgccc
     1021 ggaaactttt taacggaaca ataaacatga atgacagaaa tgcccaggtt gcatttaaag
     1081 gaagtattga tttcagagag aaactacctt tgtttgactt taatgctgta gtgaaaaatg
     1141 cagcactgac aaagcttaat ctcattaaac gcgatacttc tgcactgctg tcaactgagg
     1201 ctaacttgaa catgcgcgga tttaattttg atgagtttga aggaaagtta caactgaaaa
     1261 acattagtta ttcagaagaa tttaaatctg cttttgtaga aaaaatattg gttaaatcag
     1321 gaatggaaga taacaacaga aacctaaatg tcacctccga tttactggac ttaaacatta
     1381 atgggcagta catattatca aaaattgttt attcattcgg tcatgtgtta aactgccatc
     1441 tgccaccatt catgcttaaa ctttcacctg atatggaaaa gacgaatact gttttttctt
     1501 tcagcggaaa aattaaagat gtgaatccta tattgaacat ctttactccg gaattggctg
     1561 taagcaatca aacagtgttt tcaggaagac tgaaccgtca gcattgcgaa attgaaatgg
     1621 atgcaacatc accttggatt aattacaaaa atttcgaaaa caaaaacatt caactcacag
     1681 caaacaataa gaacagttta ttgaatattg acttatctgc cactcaggta aaaatttctg
     1741 acagcttaat tctgaaaaat attttacttg atgcagaaac acaaaaaagc aaatcatcca
     1801 ttgtacttac tgttgctaac gattcgctct caacatcaaa agtgaggctt gcgacagagc
     1861 agacttacac aaatggtgca acacgctttc atttacttga atctttaata cagattaaag
     1921 gtgaacaatg gcaggttgat ccttcaaatg aaattttatt cgactcagca tctatagctt
     1981 ttgacaaggt tttatttacc aataaaagtc agtcgctcat gctacagggc aaggttagtc
     2041 ataatagtga tgatgtatta agtttaaatc tgttcaagtt taaaattccg cttctgaatg
     2101 acttacttgc cgtgtatgat ataaaagccg gtggtgcagc aagtggaaga ttaaacttca
     2161 gttcgcttta tcagaaagca ataattaccg gagaaatgcg ggttgacagc tttgcattca
     2221 atgacgaacc tttaggaaat gccaacgtaa attttgattg gctgaccgac caaaagaagt
     2281 tacatattga aggagccatt gaacaaaaca gcagaaacag tgctgttgta aaaggtgatt
     2341 atatctttaa acctaaaaat gacgaattaa gttttaatat taaagttgac aaacttagca
     2401 taacaccctt ggaaagatac cttaaagggt ttgcaagtaa aattgtagga cttgccacag
     2461 gagaattaaa tcttacagga ttggctaaag cacccaatct aaccggcaaa gcccgactgc
     2521 agcgcgcttc tattttaatt gactatctca acacacgtta ttctgtgttg ccggagcagg
     2581 atattgtttt tgatgaaaaa tatattgcat tcaacaatat tggaatttct gacgatcaga
     2641 atcacaaagg ctctgttgac ggaaaaatat ttcataatca ttttagtgat ttcactttca
     2701 gcctaagagt taatgcaaat aagcttcaat gcctgaacac caatgctttt caaaacgaat
     2761 tgttttatgg caaagccttt gcaaccggtt atgcaaaaat tatcggcact ttaaacctgt
     2821 tgaaattgga tgtagtggca cgttctgaat cgggtacaca gatattcatt cctctttcaa
     2881 accctgaaga agtaacacag agtaattata tcacatttat caatgtgaat gatacggctg
     2941 caaaaaagtt tattataaca aacaaagttg atttgacagg aatacaaatg gatttacaac
     3001 ttgaagcaac tccggaagca gagattcaat tgatatttga ttcaaaggtt ggtgatgtga
     3061 tgcggggcaa cggctcagga aatttaagga tgctgatttc gcaggcagga gaattttcca
     3121 tgtatggtga ctataatatt cttgcaggtg attatttgtt cacactacag aatattgtta
     3181 acaagagatt taaaattcaa agtggacaga ttcaatgggc aggttcgcct tatgatgcaa
     3241 caataaacat caatgccaaa tacaacctcc gtgcctctac atttgatttg ataccggact
     3301 cgtcaaaccg cagtagagta ccggtggaag tattgctgca gttaaagaac aatctgatga
     3361 gccctgacgt tgtttttgat gtcaacgtac cgaatgccac acctgcagtg cgaacgagtc
     3421 tgagtagtat tttaggtaat gagcgcgaaa tgaacaggca ggttttcagt ctgctcgtgt
     3481 taaaccgctt ttcaccacct gaagggaaaa acacctctga agaaagtggc tccggcaatg
     3541 cagtaggtca gaatctgagt gaatttgtat cgcagcagct atcaaactgg gcatcgcagt
     3601 taagcgataa atttaatatt ggtgttaact atcgtccggg tgatgcactc aacagagatg
     3661 agtttgatgt ttcaattgca actgaattgt tcagaaacag agttgccgta gaaagtaacg
     3721 ttggtgttgc caacaacagc aatcagactt caagtatcat tggcgacttt caggtagagt
     3781 ttaaaatcag caaggatgga aaccttcgtg caaaagcttt taataaaacc aacacaaaca
     3841 atctcatcaa caaccttaac tcacaataca ctcagggtat tggtgttttt tacagaaaag
     3901 aatttaatac ggtctcagat ttagttgagt cgttcagaaa aagaaagaaa actacatctc
     3961 caaattaaat ttccatcagt gagctgcttc ctcctaccca ggttttattc ttattgtgac
     4021 ttacgccaat cattgcaccc ttacttaatc gtgtaagatt cagcaccaac tgcggtttag
     4081 tttcagcatc atcccagata tagagtaaac gaacttcaac ttttgcagca ccatcaggtg
     4141 tttccatgca gggcacataa ttaaccttgc gctgaagcaa atattcattt cttttttcgg
     4201 caggaatatt cataatgtca gaagtagtta cttctatcag cacgccactg ccggcaaaag
     4261 aaaacaatgg cttcaacaca aaatcagtga gatctatttt tgatatgtca acatcactta
     4321 agaaataaca ttccgaagca aatctgctct ttataaaagg catggtgtac ttactgattc
     4381 tgaaaaacca gtttggatga cctgcccatt ccacatcaac attgtcggtc agattaaagt
     4441 ctttaatcag atcttttcgc tgcagcaact catcaaaaat aacccggtta taaattctgt
     4501 aaataggagt ttttacacca ttcaattcat aaaataattt tcttccctct tttattactt
     4561 tcgatataca aaccggttct actcccgtat gtgtttttgt ttcccaaaag tctatccatg
     4621 ttttttgctt ttcgggttct atttcaagca agactacatt ctccggttta tgattcccta
     4681 gtacaacttt tcgcatcagt gcgcgaagct tctctgtatt ccatccatta aaaaaatggc
     4741 tgaatccatc cggtatttga aaatatttct cataggttcg cggcaacaaa tcctgccaga
     4801 aaaaaagaga agcaaatcct tgcaattcta ttaattgtgg taaataactt ccactatcat
     4861 ctttgcagat ggcaaaatct attgcaataa aatgcgggtg attatgttca ttcggaactt
     4921 ttaatccata aggaattgcc tgttcacttt ttataagata atctttagcc aatgattgtg
     4981 ttaatatatc attaccggct tcaatgagta aatcctttaa tttttttgga ataaatacgg
     5041 gcgactctgc aattcggaat tctactattc tattatatag attatcaaaa tatgctaaaa
     5101 aatctgagta cgtttcttct ttaaattctc ttaaatattt atctctaatt tccttaatca
     5161 tgtgtcaaaa ataaaaaagg gcgacctatt taaaaatcgc cctgtcaaaa tattatatgt
     5221 tttctaaagt tttttgattt ctctttctgt cacccattta tccttaaaaa cagttttatc
     5281 atacttgata tgatatttat tttctccaac ctcaataact gttgcaggtt caggaaccat
     5341 tccgtattct acttcaactc tatcacccac tttgaattta gttaatggct gtgcaggtgc
     5401 ttcttttcca aaatccttta acctatcaat gggaacccat tcattccagc cttcatccca
     5461 accatcataa gaaacataat aggtatgatc atcggcatta atctttaata ttttgccggg
     5521 ataccaggct ccattagatt taatttgtac cttttcatta actttttgtg cataggtaaa
     5581 agttataaaa cctataaaag ctaaacaagt gattattagt tttttcattc tatttatttt
     5641 ttttcaaata tagtgttttc ttcaaattaa caatactttt ctaccggtaa tttacaaacc
     5701 tcataaaaaa tttatctgcg taaatagaat cctacctgtt taaacagaaa ttttgtttga
     5761 tttgcattct gtaaaatggt tcaggacaaa cgggctttat tctttaatta tttattttgg
     5821 ctaacagggt ttctgttacc atttgaccag ccttggataa attatgctct tggcctactg
     5881 ctattgtttt ttattttgca atggctgacc ggtaaatatc agattaaaat tcagaatcca
     5941 ttgttgtggc ttcctctctc ctacttcctg ctgcattgca taggtatgat ttatacacaa
     6001 tcgcaatatg cagggtggat tgaaatatct gcaaaaagtt cattccttat ttttccgctt
     6061 ctattctctt ctaatgcaat ttctcgcgag atgattagaa cttttaagtt attttttgtt
     6121 ttcggaagtg gaataatttg cttgtataat ctgctgcttt cattcaatca atatctgcta
     6181 catggcaaca tgaattcatt ttattatgtt gctttttcta aaggtatgca tgtgcagtat
     6241 ctgacaattt atttaaacct tgccattctg tttattttag aaatactttt taacacttct
     6301 tctcaaacaa acaaaatcaa aaaaatcttt tgggtattgt tggttttatt tttcttaatc
     6361 aacattaccc tgcttgcaag cagacttgcc accgtagtat cgtatgtaac aattttgttt
     6421 tacaccgttg taagaattat ttcatcgcca aattacatac gttattctgt tcttgcagca
     6481 ttgtttgcag cttctgtgtt gattattgat tattttgcat ttaagaaaat taaccgtttt
     6541 gagcaagtag aaatttttct aaatgaaaaa accaattttg ttgatttttc aaagactgaa
     6601 tacaatagta cgaccctaag aattcctctt tggataaatg cttatgaagt catacaacgc
     6661 aatcctctat ttggtgttgg tacgggagat gtaaaatcaa gtttagattc catgtatctg
     6721 aaaaacaaat tcacctatgc ccatcagaat catttcaacc cgcataacca gtatcttcaa
     6781 acgggagttg cttttggact gacaggaatt tctttattaa tgttaatgat gctgattccg
     6841 ctctattatg caataaggaa cagacattat attttcattg catttcaatg tattttttta
     6901 ttgaatatgc tcacagaatc aatcttagaa aggcaggccg gaattatatt attctgtctt
     6961 ttttatgcta ttttcagttc tgaatatgtt gccgcaacta taaagactgt gcctgaacaa
     7021 aacaatacgt aattttgtct tttaaattca atttatgaaa gtaccatttg caccacctcg
     7081 catggacaaa cgtgttgttg atgaagttgc agacacactt ctctcgggat ggataactac
     7141 ggggcctaag actaaaaagt ttgaaaaact gcttgcagct tactgcaatg tacctaatat
     7201 acttgcctta aattctgcca ctgcaggact tgaaataatg cttcgttggt ttggtgtaaa
     7261 agaaggtgat gaggtgattt tacctgccta tacttattct gcaacagcca atgtagttgt
     7321 gcactgtggt gcaaagcctg tctttgttga tgtaggtgct gacttcaata ttttgccttc
     7381 tgaaattgaa aaagccataa acaaaaatac caaggtgata atgcctgttg attttggtgg
     7441 tctgccatgc gattttcaaa aaatacacag catagttcaa tcagagaata tcaaaaataa
     7501 atttgtgtct gcaaccgatg tgcaaagaca attagggcgt attttaattt tgtcagatgc
     7561 ggcacattct tttggggcca ctgtcaacaa tattaagaca ggagcactta ccgatgtttc
     7621 tgtattttct tttcatgctg taaagaacct aacaactgct gaaggtggtg ccattgcatt
     7681 aaaccttcca aatcctttcg ataatcaaag tatttatgac tatttatgca tttatacgct
     7741 gcatggacag aataaagatg cactggcaaa aatgcagaag ggcaattggc gctacgatat
     7801 tattgaagcc ggttttaaat gcaatatgac cgatattatg gcttcaatag gtcttgtaga
     7861 acttgaacgt tatgcagata cattagaacg cagaaaacaa atcgtttcaa attacaatac
     7921 ggcatttgaa aagtatccat gggcagagtt gcccgaattt atttcagact ggcgcgaaag
     7981 ctcctatcac ctctatccaa tcagaataaa aaactgtacg gaaaaacaac gcgatgccat
     8041 tatgcaaaaa atgtttgatg ccgatattgc agtgaatgtt cattttattc ctgtcccaat
     8101 gttttctttt tataccaaat taggatatag tcttcaacat tatcccaaag caaaagacaa
     8161 ctacgaaaga gaaatttcgc taccgttgtt ttataatctc acagacgaac aacaacaatt
     8221 tgttattgat aatttgataa aggccgtgaa tgaaacatta gcaggctaac tacaagcaat
     8281 ttctcaaaaa caatactgca agaagaataa ggaattcatc atttaaaata ttccggctaa
     8341 ctttattttt catttattaa aacaacattc cttatcactc attattaaaa aaaacaccga
     8401 aatcaaaaaa gttgattccg gtgtttttca aacgggaata ttcttttacc gtttaataat
     8461 ttttgtgttg taaagcttat tgtcagcttt tacctgtaga taatacatgc cctgtgaaat
     8521 attattaaga ggcatcttat aaacgtcaga aatgatattt gatgctacat acaccaactt
     8581 accatctgtt gtaaataatt tgacttcagc atttttaaga tcttttgtaa ttattgttaa
     8641 ctcatcctga acagggttag gatacacagc tatactattg cctgaataaa tttctttaac
     8701 cgaagttacc attgttgatg gtggaaactg aatattatca agcatagcca aatcttgtcc
     8761 acctatacaa cacacttcat ctttaatata tgcccaagta agtgtgtgct gtcctatagt
     8821 tatcggatat gatttataag cccagctata cacaccggaa taggaatcaa tcaatgtacc
     8881 atcaatgtag aaattcagaa aatcccatcc catttcactt gatatgagca tataaaacga
     8941 aatagaatcg ttgttataaa catttactgt tattgccaga tctgaggtgt cgccatcagc
     9001 aattattgca gaacgtgcag cgtatgctcc ctgaaaagtt ccgctattgg taatagtcca
     9061 tggatgattt ccattattaa gccagggata tgtagtgaag tttccgtatt cgaaattttc
     9121 taaaatttca ccggcaacca tattaaatat cttgttgtcg gaatatggat tggcatcaat
     9181 attaaattgc atatcgaatg ttgaaccaat ctgaatgttt gaagccagtg aaacattaaa
     9241 agttgcatct acatcagata atgcctgtac aacacctgta ttgtatgatg tattgttaat
     9301 ggtaagataa gggctgctgg ttgccagtgt tgcagttgag gtagtagttg cactatgtcc
     9361 cttattgttt gtttttattg tgacatcagc agtttctcca ggttcaagaa caccattgtt
     9421 gtttcctgta gggtcagaca ctgtaatgga atatacttgc agcacaggag ctttaagtaa
     9481 atgtgttatg gaagtattcc ataccgtacc aaccgaatcg gttaatataa tatcaaaaac
     9541 tgcaggtgtg ttatcaggaa caaaagctgc aaccgtataa ttaaatgcat ttataattcc
     9601 ggcagcgttt ccactctgaa tattcacttt gtttaaacta ctgctgttta ttgttacata
     9661 aggacaagta gtagttactg taagtccaac tcctactgca tcgccctgcc caaagttttc
     9721 aagcattaag tcaacatcta caatctcgtt ataatccgga agttgatttg catttcctcc
     9781 tgcatcatta ataacattac ttgtcagcga aacgaaagga cctgttgttg ctacaacagg
     9841 aatggccacc atgtatggaa taccattaaa tgctgtgatg gtcaaataaa gcgtatcgag
     9901 agatgtaatg gtgttgaaat tcaatatcac attccctcct atcaccaatc cagttgcaag
     9961 aatttgtcca ttgctgctca aacatgcaag tgcactgtcg gtattacagg taattgctaa
    10021 ttgagaagca cctggaaaca atacaggact atgacttgcc gtaattgtat aaggcgttgc
    10081 tgtacgaaca tttaatgaag ggtcgccaaa acaatgccag gtatcagtca tttcgtaacc
    10141 tgctgcacca tactgatcat tcatgtgcat acaaccattc atactcagtc caccaaaagt
    10201 tcttttaatg tttgacgaat tggtgccaac caataagtca accatttcgt cctgagcatc
    10261 cataggtgga ttccatgatt ggttaatgct cgacataaat gtggctactg ctcctgaagg
    10321 ttgtccgtta tactgtgcac gcaaaaatga ttctgccaga caagtagcgt tggcaaattc
    10381 tccattaaca caagccacac tccagataaa tggcaacatg cctgtgtttg ttaatgctgt
    10441 tgcattggca tttgaaaaac cggttgtacc taatgatgca gaacttccat ggcctgtata
    10501 ggtaattatt gaagcaccat tatttatttc tgatgataac attgaagcag acggattgcc
    10561 tgacatatcg tttccgcctt gagagccatc atataactca gctacatcag tataagtaaa
    10621 tgccaattct tttccgcgta tcacctgttg gtgctgaaaa tccatttcat tatcatcacc
    10681 cggaccttgg tcagagccta tgcatattcc ttttgaatac caatttacac ctggcgttgg
    10741 caacatttca tactcaactg tacgttgaac ctgtgtaatt acatcagtaa cggtctgggc
    10801 gctgaatcgt ccaacaaaca cttccgcata agaatcgttc cctaaaagat atccatacga
    10861 tgggtcagat gcaccacctg ataaaacagg agtaggaatc tgctgtgcat cgcctaccaa
    10921 taaaacatat ttcagattat gcgtgttgta ataattctga ataaaagttt ttatctgttg
    10981 tactgtactt cctgctgtgg ctgcatcaac cattgaaacc tgtaaccctc tttttacttt
    11041 ccagtcaata aacggttgca tggccggcat aaaagatgca tcagaaataa tcagcatctc
    11101 gccatcttcg ctcaccggta aataattaat atatgaacct gcattgcgga aaacattctg
    11161 atatatactt gcaaaatcac gatctacatt ctgcagtgat gaagtgcgat tcaacaggtt
    11221 tgttcctgaa cctgacttgt gctgcatggt aactttaatt tttgaaataa cttttaaagt
    11281 ttgcgttaca gcgttataac tgaatggatt cagcactact gtttgtccgc gaaaatcacg
    11341 aagtataaac gggctacgta aaaagccatt gttagcagga aaataaacat tctgactgta
    11401 aatatctcca aagctgtatg gcacatctga tggcttaatg tctctgtata gatttccttt
    11461 agatggtgct atcttaatat tctgaaaagt aagttcctca atcggctcaa tattcaggct
    11521 catatctccg tcatcaggaa taatcaatga caaagctaac tttggcaagt ctggtgtgcc
    11581 ttgctccatt cttggagtca tttcggggct tgttatggaa tagtactctc cagctaatga
    11641 tttaactctg tgtttttcaa atcctgaaat agaaatttcc attacattat tacctggtgt
    11701 tccactgaca acaacagctg attttgaagg aaggtctgag gtgattttaa cccattctgc
    11761 atttccctgt atagcgaaca atgctataaa aagtgtagca acaattgtct ttttttgcat
    11821 gactaaattt gtttactcaa aattaagtct ttttttgaat acaatctttg attttaacaa
    11881 aaaaaattac aacaccccct tgttaacttt gttgaaatat ttttttatgt tagtgaataa
    11941 atgtttctaa atctgaagca aaaaactaat tttgcacgta taatttaatc gcatttattc
    12001 atggctgtat atcgttttaa agtatcgttc gaagatttcg aagatattta tcgtgaaata
    12061 gaagttacat cagagcaaac ctttgccgat ttttataagg ctattttgtc ttcaataaaa
    12121 tttgaagatc gtggtactgc ttcatttttt atgagtaatg acagttggaa aaaaggtgtt
    12181 gagatttcca atagcgataa aactgatcgt gatggaaaac cagttgcaaa aaccgataaa
    12241 tcatttctta aaagttatat tgccgatccg catcagaaaa tttatttttt aattgactgc
    12301 gaaagcgttt gggcattcca tattgagttg gtgaagattt tagctgctgc agatgtaaaa
    12361 ctatcttatc cacatgtcag aaaagtatcc ggtgatgcac ctagtcagtt taaagttaag
    12421 atagctccag tgcttgaaga tgatgatttt gttgatacag atgtcagcga tgaaagcaat
    12481 tcagttgctg atgaatttgc aggagaaaat gactatgacg aagatgacac agcaacactt
    12541 gagggcgaag aaggtgaaga aagaacagac gaagacagtg aggaatttaa tgatgttgaa
    12601 atgggtagtg atgaaaatta cgaagaaagt taaaagtatt ttccttatgc ttggaagcac
    12661 atcatcagat gtgctttttt aattttaata acaatgaaaa aaggagataa agtcagcttt
    12721 aaagatgaaa aattgaacgg tgtcattctt gaaattttac ctgctggaag cgtattggtt
    12781 gaactggaag acggttttac tatggaagct ttgcaaacac agttggtagt aactgttccc
    12841 tttataaaac aacaaataca gacagatgtc aaggaagcat tatctgaaca aaatacttca
    12901 acacataata ttcctttcga cttgaacaat aatattattg aattttatac aataccttca
    12961 aaagccgaca gagtacttac aggcagcgtt gattattttc tgacaaatgg cacacccaat
    13021 ttattattac tgattttcgg aaaacgaagc aacaacaacc acacaatatt gcataccgga
    13081 gtattggaac ccaatagtta tgtatgcctt tctacattta aacgtgatgc attaattaac
    13141 attgaatcgt tttacataca aatattaatt gctgcagatg gagaatataa aagacctttt
    13201 acaaaagata tttctgttga actacctgag ttgaatcaaa caaaaaatac agcaccggga
    13261 attctttctt cttcaaagaa aacaagtctt atcaatttga atgttgcaga tgaaattcaa
    13321 tttaaagact tattgtcaaa atttgataac agtcaatcag ctagaacttc agacaacaaa
    13381 aagaatagaa aacaacaacc tgtaagcgat tctgatatca gcaaagaaat tgacttgcac
    13441 atagaaaagc ttactgctga ctttcaaaat atgacaaaca gcgaaatgct cgcacttcaa
    13501 ataagcacat tcagaaaaga aatggacaat gctatagtaa aagactgcaa gcagttaatt
    13561 ttcattcatg gtattggtga tggtgtattg agaaatgcaa taatgaatga aacaaaaaat
    13621 tatgctgaca taaaagtttt tatggctaat catgcaaaat atggagcagg cgctttggaa
    13681 atacgctttt tataaactac gctgcttata atgtgtccta aggtttttcg cccactgatt
    13741 aaaaaactgt tcctgtgctg agtttttttc actgatgtgt tttaccttcc aggttgaaaa
    13801 ataatttttc acagtaggca tcatttcaat agtctttatt ctgctacctc ttgaaatcaa
    13861 aaaacttgtt tgatgacctt caatttgatt aatgtgcacg ttataattct tatctgcctt
    13921 ccatccatta acaccaataa tcaaatcacc aactgcaagg ccattttcaa atgccggtga
    13981 acctgttgcc acattcatca cttcgggtat tgctgcgcta tcgttggttt taaaaccaaa
    14041 atatccttct gcatacttct tagaaggaat ctcttccaat gctaaacctg caaacaacag
    14101 tacctcttca agcaaagatt gatagctgca agcattataa acaatatcat taaagaaagt
    14161 ctctaaactt ctacctgcct tttcagatgc gattcttaaa taatcagctg acgtataacc
    14221 aattttcttt ttgccaaatg cattatacaa ttccacaaaa acatcatcta atgaattttt
    14281 tccatcagaa tatttacgga tcaaaaaatc aagtattagc gacactaacg aaccttcatc
    14341 atagatagat gttttacgtc cgggaattcc ggttacatag ccatcaagcc aggtatcaaa
    14401 tgacgattgt gctactgaat aattaaaccg gccttcatta ctcagatgtt tgttgatacg
    14461 gatgtttaac tcattaaaat attcttcctc gctgaaataa ccacatcgcg ccaaaaacaa
    14521 atcgccataa tatgttgttg ctccttcata gacaaatccc gtttctgcat aatttgggct
    14581 atcataatta taaggttgca tttctaccgg tcttatggtt ttaatattcc aaacatgaaa
    14641 caattcatgt gaagcaacac ccaataactc attgtacaat acgctgtcat taagatttgc
    14701 cgaaggacca atcaccaaaa ctgtagatgc caggtgctct acaccatgat aaaaagcgtg
    14761 tggcacagcc tgaattaaaa aatgataatc ttttacagga attgatttca tcatattcaa
    14821 ttgaacctct gtaaaagatt taaaatcttt tatcagcttt tcaatatcag gtttgtaatt
    14881 accctgaatc caaacattaa atctacaatc agctaccgta tagccataat gcttcaacgc
    14941 atcagaagct ataaaaggac tgtcaaccag ttcatcataa ttcttagccg aaaaaatatt
    15001 tttttctacc ggtgtgagcg atgtagctac tttgctgaag ttgtacgcta tctcaacggt
    15061 gcaggcattg cccctattgt catcagtata aaagcaacaa tgtatgggat tgatataaat
    15121 ctgttcgtca ttgacaaagc aggcacctgc atccaactga ttgcaatagt actgatagtt
    15181 gatttttatt gtatcccttt tatttgtttg cagtagccag gagctctttg aaactttttt
    15241 ccatttcact tcctttccac taatatcctg aactttaaag ttgcgaatgt ttttcgcaaa
    15301 gtttccttct tcgtacctgc ccggtcgcca gacaggaagt tttaataatg tttcatcacc
    15361 gtttgtttgt atggttagtt caatatcaac aaagtgtgag gctgcatgag atattgaaaa
    15421 gcgataatgc atcatattaa taattcaaaa ggtaactcat ctgttcagct actttctgtg
    15481 cattgggtat catggtaaat tccaaagttg aatttaatgg tattgcaggc atattctccg
    15541 agcccatagc aacaactggt gcatctaatg ctgtgaaaca ttgttgctgt atgcgacctg
    15601 aaagtgcttc ggcaaatgag ttttttatag gctcttctgt cagcaccaaa caacgatgat
    15661 gttttcttac agactcaaat attgtagttt catctaatgg attgagtgta cgcagatcaa
    15721 taatttcaat tcttccttta aactcttgtg ctgctgcctt tgcccaataa acgcccatac
    15781 cgtaagtgat aactgtcagc gtgttttcat catgcaatgc ttctgcctgt tgcactattc
    15841 ttgcttttcc aaatggaata atataatctt catcaggttc gatggttcgt gcctcttctg
    15901 tgcctttaat cttactccag taaagtccct tgtgttcaaa tattacaaca gggtttggat
    15961 cgtaataagc agctttcatc aatcctttca ggtcggcacc tgttgacgga taagcaactt
    16021 ttacacctct gatgtttaac actactgttt caacacttga cgaatgataa ggcccaccac
    16081 ttccatacgc accaatagga acacggataa cacatgatac cggccatttg ccattactta
    16141 aataacagga acggcttacc tcagtaaaaa gttgatttaa acccggccat atataatctg
    16201 caaactgtac ttcaacaatt ggtttacatc caacagcact catgccaact gtagaaccta
    16261 taataaatgc ttcctgtatg ggtgtattaa aaacccggtc atcgccaaat gtcttagcta
    16321 acgttgctgc ctctctgaaa acaccaccaa gtctggcgcc aacatcctgt ccgtagaaaa
    16381 ggcattcagg atgtttttgc agtaattcct taacggcaaa caatgcacag tcaaccataa
    16441 ctgtaggctc cttacccgct ggttgacgta tccctttttc ttctgtaatt gttgtaggtg
    16501 cataatcatg cttaaacaaa tcttcgggca gtgggtcatc agcggctttg gcctttgcga
    16561 agtcgtcctc gacaagtgtc cttgtttctt tctttatagc atctaattca tcagaagtaa
    16621 atcctgcttc ttcaagttgt tgtataaact ttggcaacgg atcttttttc tttgcttctt
    16681 ccaaatcatg tctgtaccac tctctgcgga cacctgaagt atgatgtcca agtaatggta
    16741 ctttggcatg aattaaaaat ggtctgcgtt ctgtacggat aatatcaaga acctccttta
    16801 ttgcattata agattgaaaa aaatcggtgc cgtcaatact gcgtgtttcc aatcctttaa
    16861 atccttttgc atattcagca gcattcatgg tgcggatctc agaagcgtgt gcagaaatat
    16921 cccacttatt gtcctgaaca aaataaagta taggaagatt tttaagaact gccatttgaa
    16981 aggcttcaga aacctcacct tcagtaacac aagcatcgcc tagagaacaa actgttacag
    17041 gatattcgcc atcggcatat tccacaagtt tttggttttc gagataatgc atgccaagtg
    17101 caatccccgt agtaggtata gcctgcattc ctgtagcact actttggtgt ggaatttttg
    17161 gcatatcatc acgtctcaaa gatggatgtg aatagtaagt tcgtccacct gaaaaaggat
    17221 catcccgttt agccattagc tgtaacatca gttcataagg tgtcatccct attgaaagta
    17281 gaatgctatc atcacgataa tatgctgaga gataatcaat atttttaagc tgaagcccta
    17341 aagcaatctg tactgcctca tgtccacgcg agcaggcatg aacatagcgt gcagtaaatg
    17401 ttgcattttc ctcatacaag tcactcatgg cacgtgctgt gcacatcagc ctgaatgctt
    17461 tgagaacaat ttctgttgat ataggtgtag attccaattt tttttctgca atcatttttc
    17521 taatgctatg gaatggcttt aacttgtttt ggttttaaat ttctctatgg cgttgcgtgt
    17581 aaaatccgac ataactaatt tgccactcat ccctgctctt tccattagca atgtatccca
    17641 actttcagtt cccttccaga aaatttgttt taacattttc atagcctcag ggttactggc
    17701 agcaagttgt tgtgctagtt tatttacagc attatctaac tcttcaatgt tatcatgcag
    17761 agaagcaaac atgcctttct cttttgccca ctgcgcactt tgccagtctg tagcgtttac
    17821 tgtaagttgt gtgaatgctc cagtaccaac ttttctttct actgcagggc ccactacaaa
    17881 aggacctatt cccacagcaa gttcgctaag tttaaccgaa gccgaaacat gggctaaggt
    17941 atagtctgcc gaacatgcca aacctacacc accaccaaca gccttacctt gaactcttgc
    18001 aatgacaaac tttggtgctt tacgtattgc attgattaca cttgcaaagc ctgaaaagaa
    18061 ttctttgcct tgtgcttcac tatttatact tatcaactca tcgaatgatg ctcctgcaca
    18121 aaaggccttc tcgcccttgc tgcgaagcac tataacttta gctgattcgt tggcacctgc
    18181 ttcttcaatt gtggtagcaa gtttacgcaa caacactccg ggaagcgaat tactctgagg
    18241 gtgaaaaaaa gtaatttcag ctacctgttc ttttataatt gattcaaccg taccttcagt
    18301 catgtctgca atagttattt ctctaaaaag tttaaattat ttcattcgtg caaagataaa
    18361 aaagcccatg aagtttacca gcatattgca atttaatata gtagctttta acaatgttgt
    18421 tggttactcc tcaaaattta gatgaattct aaagttatgc ttctgatatt aattatatta
    18481 ttgcataaaa tagcaatacg aattgaccta aacacaacag cttaggtcat agaatttaat
    18541 ccaacttatg caatttaaca tccctgctca tttaaccgga ttaaccgata aggaggttga
    18601 gttgtcacgc ttaaaatttg gaataaacca aaaaaagaca gaggcaggaa actcaatttt
    18661 tagttggata gtggatttat tcaaggagcc tatgctcata ctgcttcttg cagtatctgt
    18721 aatttacctg attattggtg accatggtga agccatcttt atgtttcttg caattgttgc
    18781 agtttctgcc atttcgttct atcaggataa tagaagtaaa aaagcacttg aagccttgga
    18841 aaaattaaat gagccactga gtacagttat ccggaatgca caagttatta aagtacctac
    18901 acacgagctt gtagtaggtg atttatgtat tgtagaagaa ggaaaattga taaatgccga
    18961 tggtaaaatt gtacatagta atgatttttc cgtcaacgaa tcttcgctta ctggcgaaag
    19021 cctttcagtg tataaaaata gcgaaagcaa ggacaatctt gtttatgatg gaactgtggc
    19081 tgtctccggt cttgctgtat ttgtagttga acaaattgga aaggaaacaa gattaggaaa
    19141 aattggagtt tctattactg aaataaaaat agaatcatcg ccattacaaa ttcaaataaa
    19201 aaagtttgtc atgacaatgg ctattttcgg aagcatcatt tttcttttag tatgtattgt
    19261 caattacctg aacacaaaaa atcttattga aagtttattg agcggactaa cccttgcaat
    19321 gtccattctg ccggaagaaa ttcctgttgc atttaccacc tttatggctt taggtgcatg
    19381 gaaattaatg cgagatggcg taataataaa acgcagcaat attgtcgaaa cccttgggag
    19441 tactacagtg atatgtaccg ataagaccgg aactattact gaaaacacca tgcatcttaa
    19501 acatctgttt ctcttaaaaa ccaacaaaac atatgatgaa tcggaatttg atactactga
    19561 cttgtcaaca ctcattgatt atgccatgtg gagtagcgaa ccacttccat ttgacccaat
    19621 ggaaaaaaca cttcatcatg tttatatgca gacacaaaaa acagatgagc gcaccaacta
    19681 taaaatgata catgaatacc cattggccgg aaaacctcca atgatgacgc atatttttga
    19741 gaatgcaaac aagcaaagaa ttattgcagc taaaggtgca cctgaagcca tcatcaatgt
    19801 gtctgtactt tctgaaggtg aaaaggaaaa tattctaaat gtgattcacg attttggcaa
    19861 taaaggatat agagttttag gtgttgcgca atcaaatttt aaaggctata attttcccga
    19921 aaagcaacaa gaattaaaat tcacttttct tggtttagta gttttttatg atccaccgaa
    19981 gcaaggtatt caaaccgtat ttcaacatat ttacgatgca ggtataaaag taaaagtcat
    20041 cactggtgat aatacagaaa caaccaaaac tattgctaaa caagcgggca ttttgaatgt
    20101 tgcagaagcc attgaaggaa aagatgtggt caacctgaat gaaaaacaat tgaggcaaga
    20161 agttgaggag aagacacttt ttacccgcat gtaccctgag gccaagctct ctgtagttca
    20221 tgctctaaaa aagaaaggtg aagttgtagc catgcttggc gatggtgtaa atgatggccc
    20281 tgcattaaaa gcagcacata ttggtgttgc catgggaaac aaaggcacag aaatagcaaa
    20341 agctgcagca tcattagtta tcaccaatga cgatttagac aaattgattg taggtattgc
    20401 tgcaggcaga agaatttatt ccaatctgaa aaaagctatt cagtatatta tctccatcca
    20461 tattccaatt attctaacag ttactttacc attgttttta gactggatct tccctcacat
    20521 ttttactcct gtgcatgtaa tttttctgga attagttatg gggccaacct gttccatagt
    20581 ttacgaaaac gaaccccttg aaaagaatat catgaaacaa aaaccaagaa tcatgacaga
    20641 gacttttcta agttggaaag aacttggaat aagtattttt caaggattag cgattacact
    20701 tggtgttttg tttatttatc aatggagtgt ttggcttggc ggcaatgaag agaaaacaag
    20761 agcattagta tttacaacat taatatttgc taatgtgttt ttaagtttag taaaccgatc
    20821 tttctattat agtattttag cttgtttcaa acataaaaac atcttgtttc caattgttat
    20881 cggggcaaca ttaattatgc tttttctgat tttatatata cctgcatttg cagacttctt
    20941 taaactggca ggacttaatc ttagagaaat aataatggca attattgtgg ctgcaatatc
    21001 tgtaatgtgg atggaaatct ataaatggac taaaagacga aaatttagct aaaaattaat
    21061 ctcattttca aaagaaatat tccattttgt tcatccgtga aataagtttc ttgattatgt
    21121 ttgtagatga aacaatgcca gcaacccggc ctttgtttaa ttaaaagtta atcactaaat
    21181 caagtaagat gaataaattt attaaaatta ttgtctattc gttaggtata ttattactga
    21241 tagtaattgg atttttaacc tacgtaaaag tagctttgcc tgatgttggc ccaccacctg
    21301 atattaaaat tgtagctact gctgaaaaaa ttgaacgtgg aagttattta gcacatcatg
    21361 ttactttatg tatggactgt cattccacac gtgactggag ttatttttcc ggacctatgg
    21421 ttgcaggcac cgaggacaaa ggcggtgaaa cttttgatca gaaatttggt tttccgggta
    21481 aatatattgc acctaacatt acaccatttc atttgaaaga ctggactgat ggtgaaattt
    21541 ttcgtgccgt tacatcaggt gtttccaaag atggtcgtgc actctttcct attatgcccc
    21601 atcttcttta tggtcaatta gacaagaatg atattgaatc tattattgct tttatcagaa
    21661 ccttaaaacc tattgaaaat aatacagaag tatcatcgtc cgattttcca atgaatttta
    21721 ttatcaatac tatacctaag aaaccatctt ttacaactat tccttcgcag catgacaaat
    21781 tgaattatgg aaaatatatt gctactgctt caggatgtat ggaatgtcat accaaacagg
    21841 ttaaaggtaa aattgtgggt cagccttatg ccggtggatt tgaatttatg cttcccggaa
    21901 acactgttgt tcgttctgcc aacattactc ccgataaaga aacgggtatt ggaacctgga
    21961 ccaatcagca gtttattgaa cgatttaaaa tgtactccga cagtgtttat gccaatgtaa
    22021 aagttgcgcc aggcacattc caaacaacaa tgccatggac aatgtatgct ggtatgcacc
    22081 aagaagacct atcagctttg tttgtatatc tgcaaagcct gcccccgata aataatgcag
    22141 ttgagaaatt tactgctgca aacggaaaat aaaattttaa atcttatatg accactttta
    22201 tttctattct gagaggaata aatgtcagtg gacatcggtt gattaaaatg gatgcattaa
    22261 aagaactgtg tgcttcttta aaaatgcaac aagtaaaaac ttacatacaa agtggtaaca
    22321 ttatttttca atcaacacta cacaactgtg acgctatcag taaaaagtta gaaagtgcta
    22381 ttttaaaaaa gtttggcttt gaagtaccgg tcatcacatt gtcgcagtct gaattaaaaa
    22441 gttgtttaca agataatcct tttataaaag aaaaaaaatt agacgcagca tattttcatg
    22501 tcacatttct ttctgaacaa ccaaccaaca atgattcgat agaaacagaa gctatcaagc
    22561 taagaaacga ccgttttgtt attaaaggaa gatgtattta cctctactgt cccgatggat
    22621 acggcaacac caaactgaca aatagttttt ttgaaaacaa acttaaagtc acagccacaa
    22681 ccagaaactg gaagacagta aatgagttgt tacggttggc tgagaattag ttttgcttct
    22741 tatagtatca agctataata ctctttaaaa tctgtagcga ctccgctaac taacttctat
    22801 ttccccaact gatctttata ctttttcagt tcgttgttgt agttcgactc tgacatgctg
    22861 gtagtattct tgaagaaata aactccaccc cagttataaa ctacttttga aaacacatca
    22921 gttgtatttg acttcttaat gatggtacga tatattttgc ggttggattc atcaatagtt
    22981 tcctctgtaa gcccttctgc ataatcttta tcctcaaaca caggcaatgt agccgtattc
    23041 tgctgttttt tatcattctg ttcattcata cgtgcgagag cctgcttctg tgcttcaatc
    23101 tcacgcaaac gctgtgcgtc tgcttcttcc tgtgctttac gttttgcctc agcatcagcc
    23161 aatgcctgtg ctttagtcaa actatcctga cgtatttttt ccaatgccgc taaacgagca
    23221 cgttctgcag cttcctcttt tgctttttta tcagctaaaa cttttgcctg tgctatagaa
    23281 tcctgacggg ctttttctaa tgcagctaaa cgaagtttct cttgttctct ggcgtctgct
    23341 tctgctttgg ctatactgtc ttttcttgct ttttccaatg ctgccaatcg tgctgcttct
    23401 gctactttag ctgctgcatc tgctttggca atactatctt gtcgggcttt ttccaatgct
    23461 gccaatcttg cagtttctgc tgctttagct gctgcatctg ctttggcaat actatcttgt
    23521 cgggcttttt ccaatgctgc taaacgggca tcttctgctg cctttgctgc tgcctcaact
    23581 ttagcaatac tatcttgtcg ggctttttct aatgctgcta aacgagcttg ctcttttgct
    23641 tgctcgcgcg ccaaacgctc ctcttcggct ttagcttttg caagagaatc tttacgctct
    23701 ttttctaatg ctgcaaggcg tgcttcttct gctgcctttg ctgctgcatc tgctttggct
    23761 atgctatcct gtcttgcctt ttctaatgcc gctaaacgag attgctcttt tgcttgctca
    23821 cgtgccaaac gttcctcttc ggctttagct ttagctaatg aatctttacg ttctttttc
//
LOCUS       DAOQNP010000045        23740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_726_length_23740_cov_9.612413, whole genome shotgun sequence.
ACCESSION   DAOQNP010000045 DAOQNP010000000
VERSION     DAOQNP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23740
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_23740_cov_9.612413"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PKY09_07520"
     CDS             complement(<1..1213)
                     /locus_tag="PKY09_07520"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB12745.1"
                     /translation="MKRFFFFIFFIGIISNVSAQITASTTSGCAPLVNVNFTSPAGLT
                     NIDWDFGDMTSSNLPNPVHTFFTAGSFNVVCTGVLSGNAFIDSITINVFRKPFTSLTA
                     TPPLSGCVPLTVLFNDSSVGGGGTAIVSRQWAFGDGATNIGNNPSPTNIYSLAGSFTV
                     SLKVTDANGCDTSISVPNYINTSIQPNAVITTVPSPPIGCTPPFLVAFSASASTSNST
                     TGTGLTYFWDFGSGNTSTAVSPPSINYPTTGTFNISLTVTDNNNCPKTVTTQVFINQP
                     FASFYAVGAVNDTVCPTVMFKSQSSGTNPFYNYGDGTSGVDSIHTYAPGTYQVTLQVL
                     NGICSDDTTITIHVEDVVADFTSTPGYSCSWPFTVQYNSTTSTAGTYQWIFGDGTTSN
                     LANPSHTFYRPD"
     gene            1354..2205
                     /locus_tag="PKY09_07525"
     CDS             1354..2205
                     /locus_tag="PKY09_07525"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNB12746.1"
                     /translation="MKAATLIYVILFSCFFAVSDGNSQGICGTVVDTARIQADAAANL
                     SSSQTQRGLFFPVNCLNKTLSITAYIFRDSTAADGITQASILSALQILNNDFAPMCLS
                     FSICEFVYVNNYNYNDFYALKHENEVYNLYYTPNTINVYFSNTLYDNTNSSVCGYAHF
                     PGGRDMIFLNKSCVGDGKTFSHEMGHFFGLYHTFETANGVELINGTNCLVAGDLICDT
                     PADPNGLNGSDCQMLPYLPDPSGNWYVPQIGNIMSYYSAGCKCGFTTQQFNWMIQQFL
                     TNRNYLW"
     gene            2239..4986
                     /locus_tag="PKY09_07530"
     CDS             2239..4986
                     /locus_tag="PKY09_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB12747.1"
                     /translation="MKSRILNQFVLAFAATVLLSFNGFAQDKTKSIAAPVKVTSVEGI
                     TEYKLSNGLRVLLFPDPSKSTITVNITYLVGSRMEGYGESGMAHLLEHMVFKGSTKHP
                     NIPQELTAHGARPNGTTWYDRTNYFETFNATEENLRWALSLESDRMVNSFINKKDLES
                     EFSVVRNEFERGENSPSSVLEERVMSTAYLWHNYGKSTIGSKEDIERVPIENLKAFYH
                     KYYQPDNAVLTIAGKIDEQKTLQLVSEYFSPIPKPERKLQETFTVEPVQDGERNVVLR
                     RVGDVQVASCGYHIPAGAHADYAAIEVLIEALTNEPSGRLYKALVDTKKATSTSGYSF
                     QLKDPGYAYFYAEVLKDKSLDTAMNTMKGIFDNLSKNPITNEEAERGKNAILKQLTLM
                     FNNSDRVGLSLSEFIAQGDWRLYFIYRDAIEKVTTADVNRVAAEYFKPSNRTTGLFIP
                     DVTPDRAVIPATPDVNSLVKDYKGKKALAEAEAFDPSPSNISNRIKQATISGGAKYTL
                     LKKTTRGGNVCVNITLRMGNAAVMQNKGTAASFTASMLRRGTKNKSFQQINDEIDKLK
                     SNVGIYGDGQTVNVSVTTTKENLSGILNLIGEIIKQPAFAQAEFDKLKEERISENESS
                     RSEPQAIASRIFSKTLNPYPKDDFRYEMSFDEEKEALNKLTLADVKNFYERFYTTSNT
                     TAAVVGDFDEPSLIISLNNLLDKWGSPVLYERAKEPYFDVAAVNEKINTPDKANAMML
                     AGMNLPLKDDNVDYPALLVGNYMLGGGFLNSRLATRIRQKEGLSYGVGSQLQSSSLDE
                     SGGFLSYAIYNPDNSEKLIAAWKEEIQKMLNEGFTATEMADAVKGLIQGRSVSRSQDR
                     ELCSKLNSYSFLGRSIKWDEEFENKIKALKVEDVNAAMKKWINPNKVSFVQAGDFNRK
                     K"
     gene            5134..6639
                     /locus_tag="PKY09_07535"
     CDS             5134..6639
                     /locus_tag="PKY09_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12748.1"
                     /translation="MKAQFTQQWVNSFCCFDTLGPHTFSYPLGRYIQQDTLTLSYFED
                     NIQLFKKLDLDTGNDIQTSVLSNDTVQSPFYYGGGTTRIANHYITSLIKYDTNSGIIK
                     LYHNSVDENLSTDWTNEITVSNGSISAPQSNLAETDFFTSVVSDSVRVYKFGLNGMFQ
                     WMIAKPLLSTNSNDKPSIYRNSLDEIVVYIRNANATLTDFSTRLIKISGSSGAVLKDT
                     IYSHTTGTSHIAFQQGDTLNIAFISSAGHFISSVNIDMITMNEIKNFTSNIACFDELE
                     KFAVDSITDQYYIKSNSSFYGFSSQDSTLFKISLVPEAQGEYDFSDIAFNQQRVFLAY
                     SYFNTTNTPVNNDILVLAIDKFNGTIIGSASYNDSRNTDENYLQHYLDTNAYYVMYAN
                     NFDNASILQEETQLGIIKYNFQPQSILDIQSNEHVFFFPNPFQNKVSVHFTIASNPLS
                     YAVYNILGELIKQDYITHSKMLDFETLKHGVYSIIFRFQNSKTTVMRMVKI"
     gene            6753..11867
                     /locus_tag="PKY09_07540"
     CDS             6753..11867
                     /locus_tag="PKY09_07540"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB12749.1"
                     /translation="MKKIFGFIVFFVTLSLSNLLYGQQASSCTNLDFELGNFVGWTGK
                     TGSCCPISMFSNGIVTGQHTIMSGAGFDPHSNNQIPVVAPGGTYSARLGNDQAGSQAE
                     QLSYSFQVTPQTELFIYRYAVVLEDPVHDPIEQPRFEIRVFDQAGNPIGCGTYNVVSG
                     AGIPGFQTWFDPNIGSDIHFKTWTTVGIELSSYMGQTVTIEFSTGDCSLGGHFGYAYV
                     DAYCSSMTVTSDYCPGSTTALLMAPPGFVSYLWSNGATTQTITINNPVIGATYSVQMT
                     SVAGCVVTLNQVIAVTQLFANFNFNPGCESGTQFFDSSYVLSGSPINQWLWDFGDGTT
                     SAIQNPWHQFPSSGNYNVTLTATNAGGCTKSITKSITILPSPVSDFNFTPACPSQAIT
                     FNQNASFPSGTITSYEWLFGDGLPSSAMPDPTHVYSGMGPYDVSLIVTGNNGCLDTIV
                     KSVAPMPDPVAGYTFSVQCMNNTVTFNDVSQLTGGTITAWQWDFGDGSPLDNTANPIH
                     VYNGGGNYSVQLIVTGSNGCTDTITKGVAMASLPTAAFTNSGACESQFTAFNDASGLA
                     GGSIASWLWYFGDGTNSTLQNPFHQYSGTGSFNISLVVLGDNGCRDSIARSITVKPSP
                     VASFSSMPVCPGQQAQFNSTAVAPAGETISSWIWNFGDGSAQTAGQSVNHMFTGAGTY
                     AVMSIVGASNGCIDSVINNISTSITPVAKFSAPSGCALVPLTFSSTSTISSGTITGWD
                     WDFGDGTAHGNTATTTHAFATPGSYNVYLTVTSNNGCTHTVLHEVSVSGKPVPSFTNS
                     SSCVNDSINLNSNSTSANVSINYLKWDFGDGTFHTGTDSVFHVYSDTGFVQVGLIVGN
                     TLGCSDTVYRQIYIKPLPVAGFRWDEMCQLAAGSFYDTSALINGNISGWLWDFGSGTA
                     NTANPQFAFANAGIQPVILTVTGNNNCKATITQNITVRPKPLAQFVSSGACVNAVETF
                     QNTSSIPSGSIVSWHWDYGDGHTAITQHGQNTFTTDGNYLVHLIAISDFACKDTALDT
                     IHVFPLPIANFFAPHVCLGNATVFNDLSQPVSDSLISWSWNFGDGSTSIMQAPSHIFA
                     QDTIYPVSLTVQNEGFCSKTITKNVIIRPLPDVGFTFTNPCVGSPVYFQDTSALAGYN
                     INWWQWNFGDSTTAAIASPVHTYSLSGNVFVKLIVRSTNGCSDSVSVPVLVNHLPDAL
                     FSVGDICEQTALTPSNQSALLGGSITSTLWQWGDNSGDSIFSPTHFYQSDGNYNINLE
                     VTGSNGCKNDTTIAIMIHDKPIASFVHSNACQLAPVAFYDQSSTVNDSLQIWQWQFGD
                     GDQSSLQSPVHDFGAYGVYYTELIVTNSFGCSDTVADSVTVAPKPQAAFSSVNVCKDQ
                     PMIFNNSSSVVAGSIVANQWNFGDGSGASIANPAYTYLSTGDYEITLVVTTANQCVDS
                     VKHIYRVYQLPEAGFIADTIEGCQPLWVLFSDSSVANEGLITKWNWDFGNGATDTLTN
                     PAAIVYNQAGYFSPVLFVETDLGCRDTFLRQDYIHVFAKPEAAFVAEPLSNTILHPLI
                     EITDQSWNATSWFYDFGDTSSTTEQNPSHWYNQPEHYTIIQYVESADGCKDTALVSIE
                     IKNDFTFYIPNSFTPNQDGFNETWNAFGIGITEFHVQVFNRWGQLLFTSDDLSKGWDG
                     TYSGSKVQQGVYIYKATVRDLFNKQHFYVGNVNVIR"
     gene            11880..12170
                     /locus_tag="PKY09_07545"
     CDS             11880..12170
                     /locus_tag="PKY09_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNB12750.1"
                     /translation="MNFRIKENAPIAWIAARFLGYKTMAIVIGKTIYLHGASVKTFQS
                     DSKWMKHELKHIEQFKRYGFIRFIFFYLSESIRHGYINNRFEIECREAENQD"
     gene            complement(12195..13073)
                     /locus_tag="PKY09_07550"
     CDS             complement(12195..13073)
                     /locus_tag="PKY09_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNB12751.1"
                     /translation="MTKRSVEIVVSPREPHFVGDGFRVHNFIPSGYRLDMQRMNPFIM
                     LDYNSKFYFDPSDTPRGVGVHPHRGFETVTIAYKGKVEHHDSSGGGGVIGEGDVQWMT
                     AASGVLHKEFHEKEWSKTGGEFQMVQLWVNLPAKDKMSKPKYQALENSKINRYKSEDL
                     KTEIEIIAGEYKGIKGSASTFTPLHLYNVKMTKYAKTEMNLPKNFNTALLVIEGSITI
                     NGTEKVDTNHFALMANDGESFTIDANENAVYLVLSGEPINEPIAAHGPFVMNTRQELV
                     QAFEDFNQGRFGTLEE"
     gene            13269..13661
                     /locus_tag="PKY09_07555"
     CDS             13269..13661
                     /locus_tag="PKY09_07555"
                     /inference="COORDINATES: protein motif:HMM:NF022264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HNB12752.1"
                     /translation="MKQRISIVIGLLIMVAVVSLSFKPDNWRILGTKTVDYQLDKDVL
                     DVQLRDGAFQKLKFVVRGGALNMHKVIVHFENGGTQEIEMRHDFDKNSQSRVIDLKGN
                     KRIIEKIVFWYDTKNVSNRKAKVTVLGR"
     gene            complement(13893..14285)
                     /locus_tag="PKY09_07560"
     CDS             complement(13893..14285)
                     /locus_tag="PKY09_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB12753.1"
                     /translation="MEKSIQSYNNSQSPEEKKICDLLADSIDAVLNSSESKIWHAHPV
                     WFIDENPIVGYSKQKLGWRLMFWSGIDFEEEALTVHGKKFKDASIFYTSVEQINVKDL
                     KRWLKKSKAIQWDYKNLIKKKGKLVRIK"
     gene            complement(14377..14886)
                     /locus_tag="PKY09_07565"
     CDS             complement(14377..14886)
                     /locus_tag="PKY09_07565"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNB12754.1"
                     /translation="MKAKTAKESLTIQTEIVLPNDTNVLGNLMGGRLLHWMDVCSAVA
                     AHRHCGRVVVTASVNNVSFNQPIRLGDIVTIQAKVSRSFTSSMEVFMEVWVERNATGD
                     KIKCNEAIYTFVAVDQQGSPIAVPELIAESEEEKKRFDGALRRKQLALILAGKMKPAD
                     ATELKALFF"
     gene            14999..15724
                     /locus_tag="PKY09_07570"
     CDS             14999..15724
                     /locus_tag="PKY09_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNB12755.1"
                     /translation="MQTKLLVLTAIIGMILNGKMAHSQTPTNMNETRKIEVTGNAEMK
                     VIPDEIFVVITIQEYTENKKKTDINEVRKNFLAACTKAGIAKENISIQGMNGYNNSGW
                     YWQRKKKTPDFVASSSYIIKFNSGEQIERLMSQLDDKGTQNMYISSTSHSKMEEFKKQ
                     VKTEALQAAKAKATYLCEGIGEKVGKVLLIKEVEGTSYAEPMMMKSRMMEASMADSNF
                     EPSGLEFQKITIQMQILAQFAIQ"
     gene            15731..17416
                     /locus_tag="PKY09_07575"
     CDS             15731..17416
                     /locus_tag="PKY09_07575"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB12756.1"
                     /translation="MQNNKGRKRGYSDKPQAGKYKSEGRPSRGKKFGSNTDKPKEEFV
                     KSGNSYSKNKFSGKRRFDANDSGFERKRKNFGSDDNTGFEKKRSSRFEGKKSVSENEG
                     GERRERTRFKKEGGFEREKRSFKSSDNPRPYRKRNEGFEGDEKPRFRKEGGFEREKRS
                     FKSSDSPRPYRKKGEGFEGGESPRFKKEGGFEREKRGFKSSDSPRPYRKRDEGFEGDE
                     KPRFKKRSDFGGTGKFEKSKGFKRNVDGFESDEKPRRFNKSFDGDSREKRSYDRKERS
                     SGRFDKPYRKKDDNRKYNKKSKEASGQLSDGLVRLNRYIANSGICSRREADELITAGL
                     VSVNGEIVTTMGTKVSQDDVIKYNGETIRNEKPMYLLLNKPKDYITTMDDPQDRRTVM
                     HLIAGACKERVYPVGRLDRNTTGVLLFTNDGDLAKKLTHPSYEVQKIYHVELDRNLKS
                     SDKEKIKEGVMLDDGMAVVDDIQYDGGFNDKKQIGVEIHSGKNRIVRRIFESMDYNVI
                     KLDRVVFAGLTKKDIARGKWRFLSEMEVNALKMATSRVKPKDKEPRKRISRSK"
     gene            17433..18605
                     /locus_tag="PKY09_07580"
     CDS             17433..18605
                     /locus_tag="PKY09_07580"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB12757.1"
                     /translation="MSHSAFHGLIIASGIWRAVLMTPGGELPFNFETKEVNGRSEIFI
                     NNGDENIEVNEISYANDSLIIQLPVYDSEFRVSVAGDSMKGYWYNNSRKNYAPMPFKA
                     FLGENYRFIKNAKSEIKLPEKWEWKFSPDTKDSSEAVALFKQDGTDVRGTLLTESGDY
                     RFLHGSLNNDSLFLSYFDGAFAYLFKASVKNNTMNGVFYSGHHWQQPWQAVVNENAEL
                     PDPFKLTFLKSDTTPFSFSALRLDSTVFSFPNKELKGRVVIVEMMGTWCPNCMDETAF
                     LSDYYAKIKNQDLTIIGLAFEKTSDFRKSVSNVKRVKDRYHVQYETLIAGNREGAASK
                     MSMLSKINGYPTTIFIDRKGKVRKIYTGFNGPATGAFHERTKDDFIRIINTLLSEK"
     gene            18737..23164
                     /locus_tag="PKY09_07585"
     CDS             18737..23164
                     /locus_tag="PKY09_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HNB12758.1"
                     /translation="MKRIYLILVVLLFASQAIYAQCTLTNATTCSCLGGGTNCDLLPD
                     ITASKKPLTILGSNGVIEYSQSGNGANNGRLRITVSTPNIGHGPMETRSSNIFVCGTD
                     TFVGTAPSICPDNITYPKIIINQRIYHKNGNSMSYYDRPAGTMTYHPTHGHQHVDNWG
                     VYTLRTPTADPNPVNWPIVGNGAKLAFCLLDIGACASSNGSGLCIADNGDTLDTPAEF
                     YGNYGLGGGSYGCSATLQGISAGYFDTYNQSLDGMWINIPPGTCNGTYYIVCQQDPDN
                     YFLEENDNNNVMAVPFTLTKQGGVVPTITPSGPTTFCAGGSVTLTCSSASDYLWSNGA
                     TTQSITVSQGGTYTCTVNNTSTCATASTPITVTVNSFPVTASANSANVCAGDPVQLNG
                     SATSSGTVTVVQTFSNNTAYPIPDNNSTGVQSPITVSGINPTALSSGVVVSVNANITH
                     TYDGDIELRLVAPSGQYAVLSNRRGGSGDNYTNTTFAMNGPMAITSGSPPYNGTFIPE
                     GNFNSLTGNVNGVWKLHVIDRASVDVGTIQNWTLKINSQVPTSISYSWTSNPSGFNST
                     NQNPIANPAQTTTYTVSAVESTTGCAGTGSVTVNVANPNVSVSGSSTICAGNSTTLSA
                     SGASTYSWSPATGLSATTGSSVTASPTQTTTYSVIGMLNGCSDTTQITVTVNPVPTNV
                     SALASPGSLCVGDILLLNGFGTDVTSWSWTGPDGFSSTQSAPSIINIQPVQAGNYTVT
                     GSNACGSASAQVIVDVKPTPYALVNITYPNGHCAGSYALVCASDTSNGAYAPYTYHWD
                     DNQTTSCINQLILANPLPLHGPSVTIVNSFGCASTNATLWSLPFGPVADSIQNPQTIC
                     QGGSYAINGNIYTAAGTYYDNFINISGCDSVVITQLTVTPSLSINVSGNNTLCEGQST
                     TLTATGASTYSWSPSTGLNTTTGATVSASPTATTTYVVTGTANGCSNSTTFVLNVNPN
                     PVIQTSGDVDVCSGQFVTLSASGASNYSWSPSTALNTTTGTTVVANPTSTITYTVIGT
                     DNGCSASSSITVNVIAQPVVNVSASTTSICNGQSATLTASGADTYTWSPSTGLNTTTG
                     AIVIATPATTTTYIVTGFVAGGCFSTNTITINANTGGAAPAMPGLIVGNKKPCPTVNE
                     NYSVTLVANAIGYTWTVPAGVTIVSGQGTNNIQVSFGSNFSSGSVSVVANGACGVSSK
                     RSLTITKNMPATPSTISGPVTGLCNAAGNFTSATVSGATTYTWSVPTGVSIVSGQGTK
                     IASINVSSSFSSGNVCVTADNSCMSSKPRCVAIKKTPSVPTTLTGPTTVCAGQQNVTY
                     STNTVFGANNYQWIVPSGSIITSGQGTTSITVNFGTNPGSVGVNAYNDCGHAGRKAVH
                     VAFNCRLGEMSSIASDIEVSPNPTSTGIVNLKVNSEVNGIALIKMTDILGKSVLIKNI
                     ANSIGTNNHKLDLSKFNKGIYLLTVDTGSKKQTLKVVIQ"
     gene            23244..>23740
                     /locus_tag="PKY09_07590"
     CDS             23244..>23740
                     /locus_tag="PKY09_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB12759.1"
                     /translation="MILFSVQGYAQQVFTNYSTANSPLPENSVRCIEVDHIGRKWIGT
                     DYGLAVFDDVNWNVYLPFTSGLPAMSVRAIAFDSLNNAWIGTLGGGVAKFDGTNWVVY
                     DDTNSPITENSIRALAFDSAGALWIGNQDGLVKFDGTNWIVYSAASSIIQNTIASIHT
                     SKNRKV"
BASE COUNT         7552 a         4253 c         4738 g         7197 t
ORIGIN      
        1 tatcaggacg ataaaaagta tgacttggat tagcaaggtt tgaagttgtg ccatcaccaa
       61 aaatccattg gtaggtacct gctgtagaag ttgtactgtt atattgaacg gtaaacggcc
      121 aactacaact gtaaccgggt gtcgaggtaa agtcagcaac aacatcttct acatgaatgg
      181 taatggttgt gtcatcagaa caaattccat tcaaaacctg caaagttacc tgataggttc
      241 ccggtgcata ggtgtgaata ctgtcaacac cagaagtgcc atcgccataa ttatagaagg
      301 gattggtgcc gctagactga cttttgaaca tgactgttgg acaaacagta tcattaaccg
      361 caccaactgc ataaaacgaa gcaaaaggtt gattaataaa aacctgcgtg gtaactgttt
      421 taggacagtt gttattatcg gtaacagtaa gagaaatatt aaaagttccg gttgttggat
      481 agtttattga tggaggtgat acagctgttg atgtgtttcc actaccaaaa tcccaaaagt
      541 aggtaaggcc tgttcctgta gtagaatttg atgttgaagc agaagcagaa aaggcaacca
      601 aaaatggagg tgtgcaaccg ataggtgggc tgggcactgt tgtaattact gcattaggtt
      661 gaatgctggt attaatataa tttggtacgg aaatagaagt gtcgcaacca ttggcatcgg
      721 tcacttttaa tgagactgta aaacttccgg caagagagta aatgttcgtt ggtgaaggat
      781 tgttgccaat attggttgcg ccatcaccaa atgcccactg cctcgagaca attgcagtgc
      841 caccaccacc aacagatgag tcattaaaaa gcactgttaa cggaacacat cctgaaagcg
      901 ggggtgttgc tgtaagtgat gtaaatggtt ttcggaatac atttattgtt atcgagtcaa
      961 taaaggcatt tcctgataac acacctgtgc atacaacatt aaaacttcct gcagtaaaga
     1021 aagtatgtac gggattaggt agatttgatg atgtcatatc accaaaatcc cagtcaatat
     1081 ttgtcagacc tgctggcgag gtaaaattaa catttaccaa aggcgcacag ccacttgtgg
     1141 tgcttgcagt aatttgtgca ctgacattgg atataatgcc tataaaaaaa atgaaaaaaa
     1201 agaatctttt caagatgtgt gaagaaaaaa gtgaaatgtt aatttttgtt ttcaaattta
     1261 tggcttttcg ttaacgatgt aaaaaattta tatgtttgta gtgtctgtat gtggtaagat
     1321 gaattttcaa tcactcaaaa gtatagtctt tgcatgaaag cagcaacttt aatttatgtc
     1381 attttatttt cctgcttttt tgctgtttcg gacggcaatt cgcagggaat ttgcggaaca
     1441 gtggttgata cagccagaat tcaggccgat gcagcagcta atttaagttc ctcgcagact
     1501 cagcgcgggc ttttttttcc tgtaaactgt ttgaataaaa cactttctat cacagcctat
     1561 atttttcgtg acagtacagc tgccgatggc atcacacagg catcaatttt gtcggcactt
     1621 caaattctga ataatgactt tgcgcctatg tgtttgtcgt tcagcatctg tgaatttgtg
     1681 tatgtaaata attacaatta caatgatttt tatgcattga agcatgaaaa tgaagtttac
     1741 aatttatact atactcccaa tacaataaat gtttatttca gcaatacatt atacgacaac
     1801 acaaactctt cagtttgtgg ctatgcgcat tttcccggag gacgcgacat gatttttcta
     1861 aataaatcct gcgttggtga tggcaaaacc ttctcacatg aaatgggaca tttttttgga
     1921 ctttatcata ctttcgaaac tgctaatgga gtagaattaa ttaatggcac aaattgtctt
     1981 gtagccggtg atttgatttg cgataccccg gcagacccta atggattaaa cggatcagat
     2041 tgtcagatgc ttccttattt gcctgaccct tccggcaact ggtatgtacc gcaaatcggt
     2101 aacatcatgt catattattc tgccggttgc aagtgtggtt ttaccacaca gcagtttaac
     2161 tggatgatac aacaattcct gactaacaga aactatcttt ggtaacaata tttttatatt
     2221 agtttaactc acatcattat gaaatcacga atattaaatc agtttgtgct tgcatttgcc
     2281 gctacggtat tgctttcctt taatggattt gcacaggaca aaaccaaatc aatagctgca
     2341 cccgttaaag ttacttccgt agaaggaatt acggagtata aactcagcaa tggattgcgt
     2401 gtattgttgt ttcccgatcc atcaaaatca accatcactg taaacattac ttacttggtt
     2461 ggttcacgca tggagggtta tggagagtca ggaatggcac atttattaga acacatggtg
     2521 tttaaaggtt ctactaagca tcctaatatt ccgcaggaac ttacagcaca tggtgcaaga
     2581 ccaaatggaa ctacatggta tgacaggaca aattattttg aaacatttaa tgccacggaa
     2641 gaaaatcttc gctgggcttt aagtctggaa agcgacagaa tggttaattc attcatcaat
     2701 aaaaaagacc ttgaatcgga gttctctgta gtacgaaatg aatttgaaag aggagaaaac
     2761 agtccgtcaa gtgtattgga agaaagagta atgtcaacag cttatctatg gcataactat
     2821 ggaaagtcaa ctatcggatc aaaagaagat attgagcgtg ttcctattga aaacctgaaa
     2881 gcattttatc ataaatacta tcaacctgat aatgctgtgc ttactattgc cggaaagatt
     2941 gatgagcaaa aaacattaca attagtaagt gagtatttta gtcctattcc aaaacctgaa
     3001 cgtaaattgc aggagacatt tacagtagaa cctgttcagg atggcgaacg taatgttgtc
     3061 ttacgcagag ttggtgatgt gcaggttgcc tcttgcgggt atcatattcc tgctggtgct
     3121 catgccgact atgcagctat tgaagttttg attgaagcat taaccaatga accttcagga
     3181 agattatata aagcattggt ggatacaaaa aaagctacaa gtacatcagg ttattcattt
     3241 caacttaaag acccgggata tgcctatttc tatgcagaag tcttaaaaga caagtcactt
     3301 gacacagcca tgaataccat gaaaggtatt tttgataacc tgagtaaaaa tccaatcact
     3361 aacgaagagg cagagcgtgg aaagaatgct attctcaaac aactaacgct gatgtttaac
     3421 aacagtgata gggtaggact ttcacttagt gaatttattg cgcaaggcga ctggcgttta
     3481 tattttattt atcgtgatgc aatagaaaaa gttactactg ccgatgtaaa cagagtggca
     3541 gcagagtatt ttaagccaag caaccgcact acaggacttt ttattcctga tgtaacccct
     3601 gacagagctg tgattcctgc aacaccagat gttaattcat tagtaaaaga ttataaagga
     3661 aagaaagcct tagcagaagc cgaagcattt gatccctcgc catcaaatat cagcaacaga
     3721 ataaaacagg caactatctc cggtggtgca aaatatacgt tattgaaaaa aaccactcgt
     3781 ggaggtaatg tctgcgtgaa cataacattg agaatgggca atgctgctgt gatgcaaaac
     3841 aaaggtactg ctgcatcatt cacagcttcg atgttacgaa ggggaacaaa gaataaatca
     3901 tttcagcaaa ttaatgatga gattgacaag cttaaatcca atgttggtat ttatggtgac
     3961 gggcaaactg ttaatgtatc agtaacaact actaaagaaa acctcagtgg cattcttaac
     4021 ctgataggtg aaattattaa acaacctgca tttgcacagg ctgaatttga caagttaaaa
     4081 gaggaaagaa tttctgaaaa tgaaagctca cgcagcgagc ctcaggctat tgcatccaga
     4141 atattcagta aaacgcttaa tccttatcct aaggacgatt tccgttatga aatgagtttt
     4201 gacgaggaga aagaagcatt gaataaactt actttggctg atgtaaagaa tttttatgaa
     4261 aggttttata ctacttccaa tacaacagca gctgtagttg gtgattttga tgagccatca
     4321 ttaattattt cacttaacaa tttacttgat aaatggggtt ctccggtgct ttatgagcgt
     4381 gctaaagaac cttattttga tgtagcagca gtgaacgaaa aaataaacac accggataag
     4441 gcgaatgcaa tgatgctggc tgggatgaat ttgccactta aagatgacaa tgttgactat
     4501 cctgcattgc ttgttggcaa ttatatgctg ggaggaggat tccttaactc acgacttgct
     4561 acacgcatca gacaaaaaga aggcttaagt tatggagttg gctctcaatt gcaatcaagc
     4621 tcattagatg agtcaggtgg ttttttatcc tatgcaattt acaatcctga caactcagaa
     4681 aaactgatag cagcatggaa agaagagatt caaaaaatgt tgaacgaagg gtttactgca
     4741 actgaaatgg ctgatgcagt gaaaggatta attcaaggtc gctcggtaag tcgctcacaa
     4801 gacagagaat tgtgtagcaa acttaacagc tattcctttt tgggtcgttc tattaaatgg
     4861 gatgaagagt ttgagaataa gattaaagca ttgaaggttg aagatgtaaa tgcagcaatg
     4921 aaaaaatgga ttaatccgaa taaggtttct tttgtgcaag caggagattt taataggaaa
     4981 aagtaattgt taaatagaaa ttttatcaaa catctctttg cagaaggaga aatgatttgt
     5041 gcagtgttag catctgtatt ttaaaaattc cgagtaaaat tttgcagttt gaagaatatt
     5101 ataatagtat ttttcttgtt cacttgtttt acggtgaaag cacaattcac tcagcagtgg
     5161 gtgaacagtt tttgctgctt cgatactctt gggccacata cattttctta tccattaggc
     5221 aggtatatac aacaagacac actcaccctt tcttattttg aagataatat acagctgttt
     5281 aaaaaattgg atttggatac tggcaatgat attcaaacta gtgtactctc caatgatacg
     5341 gtacaatcac cattttatta tggtggtgga accacgagaa ttgccaatca ttatataacc
     5401 tcactgatta agtatgacac aaattccgga attataaagt tgtatcataa ttcagtagat
     5461 gaaaatctgt caacagactg gactaatgaa ataacagttt caaatggttc aatatcggca
     5521 cctcagagta atttagcaga gactgatttt ttcacatctg ttgtttctga ttctgtcaga
     5581 gtttacaaat ttggtttgaa tggaatgttt cagtggatga tagcaaagcc gttgttaagt
     5641 accaatagta acgataaacc ttctatttat cgtaattctt tagatgagat agtggtgtat
     5701 atacgcaatg caaatgccac cctgactgat tttagtactc gacttattaa aattagcggc
     5761 agcagtggtg ctgtgttgaa agatactatt tacagtcaca caacaggtac ttctcatatt
     5821 gcctttcagc agggagatac actcaatata gcttttatta gtagtgccgg tcacttcatt
     5881 tcctcggtaa atattgatat gatcacaatg aatgagataa aaaattttac aagtaatatt
     5941 gcctgttttg atgaacttga aaaatttgct gttgatagta taaccgatca gtattacata
     6001 aaatccaata gctcttttta tggtttctca tcacaagatt ccacactgtt caaaattagt
     6061 ctggtaccgg aagcccaggg tgagtatgat tttagtgaca ttgcttttaa tcaacaacgt
     6121 gtttttttgg cttacagtta ttttaacact acaaatactc ctgttaacaa tgatattctt
     6181 gttttggcca tagacaaatt taatggaaca ataatcggta gtgcaagcta taacgatagc
     6241 cgcaatactg acgagaatta tcttcaacat tatctcgaca caaatgctta ctatgtaatg
     6301 tatgctaata attttgataa tgcatccatc ttgcaggaag aaacacaatt aggaatcata
     6361 aagtataact ttcagcctca aagtattttg gacatacaat ccaatgaaca tgttttcttt
     6421 tttccaaacc cttttcaaaa taaagtatct gttcatttta cgatagcgag taatccatta
     6481 agttatgctg tttacaatat acttggagag ttgataaagc aagattacat tactcatagc
     6541 aaaatgttgg attttgaaac gctgaagcat ggagtttatt ctattatatt caggtttcag
     6601 aatagcaaaa caacagtcat gcgaatggta aaaatataaa tctgttattg catttacata
     6661 aagaatttag ggtgaaatat tcgctttttt aaaaaaatct gttactttta cccaattaaa
     6721 gaaccggcca actaagcaag atatatctgg tcatgaaaaa gatatttggt tttattgttt
     6781 tttttgtaac gttatcctta agtaatttgc tttatgggca acaggcaagc agttgtacca
     6841 atcttgattt tgaactcgga aactttgtag gctggacagg aaagacaggc tcatgttgtc
     6901 caatcagtat gttttcaaac ggtattgtca ctggtcagca tactattatg tccggtgccg
     6961 gatttgatcc tcactctaac aatcagatac ctgttgttgc acccggagga acttactctg
     7021 cacgtttagg aaatgatcag gcaggctcgc aagcagagca gctttcttat tcctttcagg
     7081 ttactccaca aacagaatta tttatttatc gttatgctgt tgttttagaa gaccctgtgc
     7141 acgaccctat agaacaacca cgttttgaaa tccgtgtatt tgatcaggca ggaaacccta
     7201 ttggctgcgg aacttacaat gtggtttcag gtgccggcat tcccggcttt caaacctggt
     7261 ttgatcccaa tattggcagc gatattcatt ttaagacttg gacaacagta ggcattgagc
     7321 tttcatctta tatgggtcaa acagttacta ttgaattttc tacaggtgat tgttccttgg
     7381 gtggccactt tggttatgct tatgtggatg cctattgcag cagcatgaca gttaccagcg
     7441 attactgccc aggtagcaca acagcactat tgatggcacc tcccggtttt gtatcttatc
     7501 tgtggagtaa tggcgcaaca actcaaacaa tcactatcaa taatcctgtc attggtgcta
     7561 catattctgt tcagatgaca tctgtagccg gatgtgtggt aacattgaat caggttattg
     7621 ccgtgacaca gttatttgct aatttcaact ttaatcccgg ctgcgaaagc ggcactcaat
     7681 ttttcgattc atcatatgtt ctttcaggtt ctcccattaa tcaatggttg tgggattttg
     7741 gtgatggcac cacttctgca atacaaaacc cgtggcatca gtttccgtct tcaggaaatt
     7801 ataacgtaac actgacagca accaatgcag gtggttgtac caagtcaata accaaatcta
     7861 taactatttt accatcgcct gtttcagact ttaactttac acctgcctgc ccaagtcagg
     7921 caataacttt taatcagaat gcaagttttc catccggaac aataacaagt tatgaatggt
     7981 tgtttggtga tggacttcct tcatcagcaa tgcctgatcc gacacatgtt tacagtggta
     8041 tgggacctta tgatgtttca ctgattgtta caggaaataa tggctgttta gatactattg
     8101 taaagtcagt ggcaccaatg cctgaccctg tggcaggata tactttcagt gttcagtgta
     8161 tgaataatac cgttacgttt aatgatgttt ctcaactgac aggtggcacc ataactgcat
     8221 ggcaatggga ttttggtgat ggctcaccat tggataatac tgcaaatcct attcatgttt
     8281 ataacggtgg tggcaattac tcagttcaac tgattgttac aggaagcaac ggatgtactg
     8341 atacaattac taaaggtgtg gctatggcaa gcttgccaac agcagctttt accaatagcg
     8401 gtgcatgcga atcacagttt acagcattta atgatgcatc tggtcttgca ggtggtagca
     8461 ttgcatcatg gctgtggtat tttggtgacg gaacaaattc aactttacaa aatccatttc
     8521 accaatatag tggcacagga agttttaata tttcattggt tgtgttaggt gataacggtt
     8581 gccgcgattc tatagcacgg agtattacag tcaagccttc tcctgtggca tcatttagct
     8641 ctatgcctgt gtgtccggga cagcaagcac agttcaatag tactgcagtt gcaccggcag
     8701 gtgaaacaat aagttcatgg atttggaatt ttggtgatgg aagtgcgcag actgcaggtc
     8761 agagcgttaa ccacatgttc accggtgccg gcacttacgc agtgatgagt attgttggtg
     8821 cttcaaatgg ttgcattgat agtgttataa ataatatttc cacttcaata acacctgtgg
     8881 ctaagttttc tgcaccatca ggttgtgcct tagttccatt aactttttcc agtacatcta
     8941 caatatcttc aggtaccata acagggtggg attgggattt tggtgatgga actgctcatg
     9001 gcaatactgc aacaacaaca catgcctttg caacacccgg aagttacaat gtatatttaa
     9061 ctgttacaag caacaatggt tgtacacata cagtattgca cgaagtgagt gtaagtggta
     9121 aacctgtacc ttcgtttacc aactcttcaa gttgtgttaa tgacagtatc aatctcaaca
     9181 gtaattcaac atctgcaaat gtcagtatca attacctgaa atgggatttt ggtgatggca
     9241 ccttccacac cggtacagat agtgtttttc atgtttattc agatacaggg tttgttcagg
     9301 tcggattaat agtagggaat accctcgggt gcagcgatac tgtttacaga cagatatata
     9361 ttaagccatt gccagttgcc ggattccgat gggatgaaat gtgtcagtta gcagccggaa
     9421 gtttttatga tacttcagca ttgataaatg gaaatatttc cggatggcta tgggattttg
     9481 gaagtggcac tgccaacact gccaaccctc agtttgcatt tgccaatgcc ggaatacaac
     9541 cggttatact cactgtaaca ggaaataaca attgtaaggc aacaataaca caaaatatta
     9601 ctgttcgacc aaaaccattg gcacagtttg tttcatcagg ggcctgtgtc aatgcagttg
     9661 aaacatttca gaatacatct tcaataccat caggaagtat tgtgagctgg cattgggatt
     9721 atggtgatgg acacactgcg ataacacaac atgggcaaaa cacttttaca accgatggaa
     9781 attatctggt gcacttaatt gcaatatcag actttgcatg taaagataca gcacttgaca
     9841 ccattcacgt gtttccatta ccaatagcca acttttttgc cccacatgta tgtctgggaa
     9901 atgcaacagt ttttaatgac ttgtcacaac ctgtttccga tagtttgatt tcatggtcgt
     9961 ggaactttgg tgatggctct acttcgataa tgcaagcccc gtcacatatt tttgcacagg
    10021 atacaattta ccctgtgtca ttgacagtac agaatgaagg cttttgcagc aagactatta
    10081 ccaagaatgt aatcatcaga cctctgccgg atgtgggctt tacatttacc aatccatgtg
    10141 tcggtagtcc ggtatatttc caggacacat cggctttagc gggatataat attaattggt
    10201 ggcaatggaa ttttggtgat agtactactg cggcaatagc atcaccggta catacctatt
    10261 cactgtcggg aaatgttttt gtcaagctga ttgttcgttc cacaaatggt tgttcagatt
    10321 ctgtttcagt acctgtttta gttaatcatt tacctgatgc acttttttct gtaggtgata
    10381 tttgcgagca aactgcactt acgccaagta atcaatcagc attattgggc ggaagcatta
    10441 caagcacttt atggcaatgg ggtgataatt caggtgattc aattttctca cctacacatt
    10501 tctatcaaag cgatggcaac tataatatta atcttgaagt tacaggaagt aatggttgta
    10561 aaaatgacac tactattgcc ataatgattc atgataaacc tattgcatca tttgtacaca
    10621 gcaatgcatg tcagttggct cctgttgcat tctatgatca gtcttctacc gttaatgact
    10681 cattacaaat ttggcaatgg caatttggtg atggggatca atcatcactt caaagccctg
    10741 tgcatgattt tggtgcttat ggcgtttatt acacagaact aattgtaaca aatagtttcg
    10801 gatgtagtga cacagttgcc gattccgtta ctgttgcacc aaagccgcag gcagcatttt
    10861 catctgtgaa tgtttgtaaa gatcagccta tgatttttaa taattcaagt agtgttgttg
    10921 ccggtagtat tgttgccaat cagtggaatt ttggtgatgg ctcaggtgct tctattgcca
    10981 atcctgccta tacatattta agtaccggtg actatgaaat taccttagta gttacaactg
    11041 ccaatcaatg tgttgatagt gtaaaacata tttatcgtgt ttatcagttg ccggaagcag
    11101 gatttattgc agatactatt gaaggctgcc agccactgtg ggtattattt tctgatagct
    11161 ctgttgcaaa cgaaggttta ataacaaaat ggaattggga ttttggtaac ggtgcaacag
    11221 atactttaac aaatcctgca gcaatagttt acaatcaggc cggatatttc agtcctgtgt
    11281 tgtttgttga aacagattta ggttgccgcg atacgttttt acgtcaggat tatattcatg
    11341 tttttgcaaa acctgaagcg gcttttgtag cagagccatt aagcaataca attttacatc
    11401 cattgattga gattacagat cagtcgtgga atgcaacatc ctggttctat gattttggag
    11461 atacatcaag tacaacagag caaaatcctt cgcattggta taatcaaccg gaacactata
    11521 ccattattca atatgtagag agtgcagatg gttgtaagga cacagcatta gtcagtatag
    11581 aaataaagaa tgattttact ttttacattc ccaattcttt cacaccaaac caagatggct
    11641 tcaacgaaac atggaatgct tttggtattg gtattacaga atttcatgtg caggttttta
    11701 atcgctgggg ccagttatta tttacctcag atgatttaag taaaggatgg gatggaacat
    11761 attcgggaag caaagtgcaa caaggtgttt acatatataa agctactgtt cgtgatttat
    11821 tcaataaaca acatttttat gttggaaatg taaatgttat cagatagtaa gtcctgtaaa
    11881 tgaatttccg aattaaagag aatgcaccaa tagcatggat tgcagcccgg tttttgggct
    11941 acaaaacaat ggcaatagtc attggaaaaa ctatttacct gcatggtgct tcagtcaaaa
    12001 cttttcagag tgattctaaa tggatgaagc acgaactcaa acacattgaa cagttcaaac
    12061 gctatggatt tatccgattt attttttttt atctttctga atctatccgt catggttata
    12121 tcaataaccg ttttgaaatt gaatgcagag aagcagaaaa tcaggattga tgattgtgca
    12181 acgatggaag tatgttattc ttccaaagtc ccgaatcgtc cctgattaaa atcttcaaat
    12241 gcctgcacca attcctgtct tgtattcatt acaaatggac cgtgtgctgc tataggctca
    12301 ttgataggtt caccactaag tacgagataa actgcatttt cgtttgcatc aattgtaaaa
    12361 ctttcaccgt cattagccat aagtgcaaaa tgattggtat ctactttttc agttccatta
    12421 attgtgatgc ttccttcaat aaccaataat gctgtattga aatttttagg cagattcatt
    12481 tcagttttag catattttgt cattttaaca ttataaagat gtagtggtgt aaaagtagag
    12541 gcacttcctt taataccttt atattctcct gcaataattt caatttctgt tttcaaatct
    12601 tctgatttgt aacgattgat ctttgagttt tctaaagcct gatatttggg tttactcatc
    12661 ttgtcttttg caggaaggtt gacccacaac tgtaccatct gaaattcacc acctgttttg
    12721 ctccattctt tctcgtgaaa ttctttatgt aacacaccac ttgctgcagt catccattga
    12781 acatcacctt caccaataac gccaccgcca ccactgctat catgatgttc tactttacct
    12841 ttataggcta tagtaacagt ttcaaatccc cgatgtggat gtactccaac tcctctggga
    12901 gtatcgcttg gatcaaagta aaatttagaa ttataatcta acatgatgaa tggattcatc
    12961 cgttgcatgt caagacgata accgcttggt ataaagttat gtactctaaa gccatcacct
    13021 acaaaatgtg gttcacgcgg actaacaact atttctacag atctttttgt catgatattt
    13081 aaattttata gtgcaaaatt attgtcaatt ctttctattg ccaataacat agattaagtt
    13141 tctaatttta tgcctgttgc aagtattaaa acttatttgg aacaggaaaa attcatttaa
    13201 cactttaaac cattgcctcc taaaaatgtc tatcaatttg aaatagcaac aattttaatt
    13261 ttaaaaatat gaaacaaaga atttcaatag taattggctt attgattatg gttgccgtag
    13321 tcagtttatc ttttaagcct gataattggc gaattttagg aacaaagaca gttgactatc
    13381 agttagacaa agatgtttta gacgtgcagt tgcgggacgg tgcttttcag aaattgaaat
    13441 ttgttgtcag aggtggagca ttaaatatgc ataaagttat tgttcatttc gagaatggag
    13501 gcacacaaga aattgaaatg cgtcatgatt ttgataagaa cagccaaagc agagttatag
    13561 acctaaaagg aaacaaacgc atcattgaaa agattgtttt ttggtacgac actaaaaatg
    13621 tttcaaaccg aaaagcaaaa gtgacagtgt taggtcgctg atgaaataat attttaagaa
    13681 cataaaacct tggttagtgg gcgatctttt acaagttcgt ccactaattt atcaaagcat
    13741 ttgatttttt cagaatgtga atttcgattt cctcaattaa ttccttcaca gtaagatttt
    13801 ttttggaatc tttgagatat ataatgtata tatagttgca agactcattt ttgctacacg
    13861 ctcaatggtt tattctgctc ttttcatata gattatttaa tacggactaa tttacctttt
    13921 tttttaataa ggtttttata atcccactga attgctttag atttttttaa ccagcgtttt
    13981 aagtccttaa catttatttg ttcaactgaa gtatagaata ttgaagcatc tttaaacttt
    14041 ttgccatgca cggttaaagc ttcttcctca aaatcaattc cactccaaaa cataagcctc
    14101 cagcccaatt tttgtttgct atagccgact attggatttt catctataaa ccaaaccggg
    14161 tgcgcatgcc aaattttact ttctgaagaa tttaatacgg catcaataga atccgccaat
    14221 aaatcacaaa ttttcttttc ttccggtgat tgactgttat tgtaactctg aatagatttt
    14281 tccataccaa acaaaataac agaatcttaa tcttagaaaa aatcagtaag tcatttattc
    14341 acgacttgta caaaatgatg taggaaattt atcgctttaa aagaacaatg ctttcagttc
    14401 tgttgcatca gcaggtttca tttttcctgc aaggattaag gcaagttgct tgcgtcttaa
    14461 tgcgccatca aaacgttttt tttcttcttc gctttcagca attaattcag gtacagcaat
    14521 aggactgcct tgttggtcaa ccgccacaaa ggtgtagatg gcttcattgc attttatttt
    14581 atctcctgtg gcattacgtt caacccacac ttccataaaa acttccatgc ttgaagtaaa
    14641 ggagcgcgat acttttgcct gaatggtaac aatatctcct aaacggattg gctgattaaa
    14701 tgatacattg tttactgaag ctgtaacaac cacacgccca caatgacggt gcgcagcaac
    14761 ggcagaacaa acatccatcc aatgtagcag gcgacctccc atcagattgc caagtacatt
    14821 ggtatcgtta ggcagtacta tctctgtctg tatggttaaa ctctctttag cagtttttgc
    14881 tttcatattc tgatttttgc gactgcaaat atctgaataa ttaaacttaa aatgaacgaa
    14941 tcaaagcctg actgtggaat taaacctatc tttgcatcca cattagaaaa caattaaaat
    15001 gcaaacaaaa ctattagtac tgacagccat aataggaatg atattaaacg gtaaaatggc
    15061 acactcacaa accccgacca atatgaacga aacaagaaaa attgaagtca caggaaatgc
    15121 tgaaatgaaa gtaataccgg atgaaatttt tgttgtcata accattcagg aatatacaga
    15181 gaataagaag aagacagata ttaatgaggt gcgcaaaaat tttctggcag catgcacaaa
    15241 ggctggtatt gctaaagaaa atatttccat acagggaatg aatggttaca ataacagtgg
    15301 ctggtactgg caacgcaaaa agaagactcc tgattttgtt gcttcgtcaa gctatataat
    15361 taagtttaat tcaggagagc agattgaaag attgatgagt cagcttgatg acaaaggcac
    15421 acagaatatg tacatcagtt ccacttcaca cagcaaaatg gaagagttta aaaaacaagt
    15481 aaaaacagaa gcattacagg cagccaaagc taaagccacc tatctttgtg aaggaattgg
    15541 agaaaaggta ggcaaagtgt tgttaataaa agaagttgaa ggaacaagtt atgcagaacc
    15601 tatgatgatg aaaagccgga tgatggaagc ttcaatggca gattctaatt ttgaaccttc
    15661 aggacttgaa ttccaaaaga taaccataca gatgcaaata ttggctcaat ttgccataca
    15721 ataagcacac atgcagaaca ataaaggccg taaacgaggc tacagcgaca agccgcaagc
    15781 cggcaaatac aagtctgaag ggcgcccttc aagaggaaag aaattcggaa gcaacactga
    15841 taaacccaaa gaggaatttg taaaatcagg caacagttat tcaaaaaata aattttcagg
    15901 caaacgcagg tttgatgcaa atgattccgg ctttgaaaga aaacggaaga attttggaag
    15961 tgatgataac acgggtttcg aaaaaaagag gtcaagtcgt tttgaaggta aaaaatctgt
    16021 ctcagaaaat gaaggcggag aaagaagaga aagaacccga tttaaaaagg aaggcggttt
    16081 tgaacgagag aaaagaagtt ttaaatcatc agataaccca cggccttaca gaaaaagaaa
    16141 cgaaggtttt gaaggtgatg agaagccccg ttttagaaaa gaaggtggtt ttgaacgaga
    16201 gaaaagaagt tttaaatcat cagatagccc gcgcccttac agaaaaaaag gtgaaggttt
    16261 tgaaggtgga gaaagccccc gttttaaaaa ggaaggtggt tttgaacgag agaaaagagg
    16321 ttttaaatca tcagatagcc cacgacctta tagaaaaaga gacgaaggtt ttgaaggtga
    16381 tgagaaacca agatttaaaa agcggagtga ctttggtggt acagggaaat ttgaaaaatc
    16441 aaaagggttc aaaagaaacg ttgatggttt tgagagcgat gaaaaaccaa gacgatttaa
    16501 caaatctttc gatggtgatt caagagagaa acgttcttat gatagaaaag aaagaagttc
    16561 ggggagattt gacaaacctt atcgcaagaa agatgacaat agaaagtata ataaaaaaag
    16621 caaagaagca tcaggacaat tatctgatgg ccttgttcgc ttgaaccgct atattgcaaa
    16681 cagtggtatt tgcagcagaa gagaagcaga tgaattaatt actgcaggtc ttgtttctgt
    16741 caatggtgaa attgttacca caatgggaac caaggtttcg caggatgatg tgattaagta
    16801 caatggtgaa accatcagaa atgaaaagcc catgtatttg ttgcttaaca aaccaaaaga
    16861 ttacatcacc accatggatg atcctcagga cagacgtact gttatgcatc ttattgccgg
    16921 ggcgtgtaag gaacgtgtgt accctgtagg tcgcctcgat agaaatacaa ccggtgtttt
    16981 gctttttact aatgatggtg atttagcaaa aaaacttact catccaagtt atgaagttca
    17041 gaaaatttat catgttgagt tagacagaaa tttaaaatct tccgataaag aaaaaattaa
    17101 agaaggtgtc atgctcgatg atggaatggc tgtggttgat gatatacagt acgatggtgg
    17161 atttaatgat aaaaagcaaa tcggtgtaga aatacactca gggaaaaacc gtattgtaag
    17221 acgtatattt gagtctatgg attacaatgt aattaagtta gatcgggtag tttttgccgg
    17281 acttaccaaa aaagatattg cacgaggtaa atggcgtttt ctgagtgaga tggaagtgaa
    17341 tgctttgaaa atggctactt ccagggtgaa accaaaagac aaagaaccgc gcaaaagaat
    17401 ttcgcgaagt aaataacttt atataagtac acttgagtca tagtgcattt catggtttga
    17461 taattgcttc cggtatttgg agagcagtat taatgacacc gggcggtgaa ctgcctttta
    17521 attttgaaac aaaagaagtt aatgggcgca gtgagatttt tataaataat ggcgatgaaa
    17581 atattgaagt aaatgaaatc tcttatgcga atgattcttt aattattcaa ttaccggttt
    17641 acgattcaga attcagagtt agcgttgcag gtgacagcat gaaaggctat tggtataata
    17701 attcaagaaa gaattatgca ccaatgccat tcaaagcttt tcttggtgaa aattatcgtt
    17761 ttataaaaaa cgcaaagtct gaaataaaac ttcctgaaaa atgggaatgg aaattcagtc
    17821 cggataccaa agattcatct gaagcagtag cattgtttaa acaagatggt acagatgtga
    17881 gaggaacgct tttaactgaa tcgggcgatt atcgttttct tcatggaagc ctcaataatg
    17941 acagtttgtt tttatcttat tttgatggtg catttgcata tctctttaaa gcatctgtaa
    18001 aaaacaatac aatgaatggt gttttttatt caggacatca ctggcagcaa ccctggcaag
    18061 cagtggttaa tgaaaatgca gaattaccgg atccatttaa gctcactttt ctgaagagtg
    18121 atactacacc tttttctttt agtgcattac gattggatag tacggttttt tcatttccca
    18181 acaaagaatt aaaaggaagg gtagtaattg tcgaaatgat gggaacctgg tgtccgaatt
    18241 gtatggacga aacagctttt ttatctgatt attatgccaa aatcaaaaac caagacttaa
    18301 ccattatcgg cttggcattt gaaaaaacat cagatttcag gaagtctgta agtaatgtta
    18361 aaagggttaa agacagatat catgttcagt acgaaacatt gattgccggt aacagagaag
    18421 gggcagcatc caaaatgagt atgctcagta aaatcaatgg ctatcctact accattttta
    18481 ttgatcgaaa gggtaaagtc agaaagatct atacaggctt taatggtccg gccacagggg
    18541 catttcatga aaggactaag gatgacttta tacgcatcat caatacctta ctttccgaaa
    18601 aatagccgtt cacctatatt tttctgtttt ttgactacag attttttctg ttaatcaaaa
    18661 aaaaccctta ttcatttcag aaaaatggtt aatttgggct acttttttat caccataata
    18721 tttaaatcaa actaatatga aacgtattta cctaatactg gtagtactac tgtttgccag
    18781 tcaagccatc tatgcacagt gtacactaac caatgcaaca acatgctcat gcttaggcgg
    18841 tggcacaaat tgcgatttat tgccagacat tactgcatca aaaaaaccat tgaccatact
    18901 tggctccaat ggtgttatag agtattcgca gtcaggcaac ggggctaaca atggacgcct
    18961 gagaattaca gtatctacac caaacatagg acatggccca atggaaacac gctcgtcaaa
    19021 tatttttgtt tgcggcaccg atacttttgt tggtactgca ccatcaattt gtcctgataa
    19081 tattacctat cctaaaatta ttatcaacca aagaatttat cataagaatg gcaactcaat
    19141 gtcatattat gatcgtcctg caggaactat gacttatcac ccaacacatg gtcatcagca
    19201 cgttgataat tggggcgtat atacactgag aacaccaacg gcagatccta atcctgtaaa
    19261 ttggcctata gtaggtaatg gtgcaaaact tgctttttgt ttgcttgata taggtgcttg
    19321 tgcatcatca aacggaagtg gattgtgtat tgcagacaat ggagatacac ttgatacacc
    19381 tgcagagttt tatggtaact atggtttagg tggtggaagt tatggttgtt cggctacttt
    19441 acaaggtatt tctgcaggtt attttgatac ttacaatcaa agtcttgatg gaatgtggat
    19501 taacatccct cccggtactt gcaatggtac ttattacatc gtatgccaac aagatccgga
    19561 caattatttt cttgaagaga atgataacaa caacgtaatg gcagtgccat ttacactgac
    19621 aaaacaaggt ggtgtagttc caaccattac tccaagtgga ccaacaacat tttgtgctgg
    19681 tggcagcgtg actttgacgt gcagcagtgc aagcgattat ttatggagca atggtgctac
    19741 cacacaaagt atcacagtgt cacaaggtgg aacatatacc tgtacggtaa ataatacatc
    19801 aacttgtgct acagcatcta ctcctattac agtaacggtt aatagttttc ctgtaactgc
    19861 atctgccaat tctgctaatg tttgtgccgg agatcctgta caactgaatg gctcagcaac
    19921 aagttcagga acggtgactg ttgttcaaac tttttctaac aatacagctt acccgattcc
    19981 agataataat tcaacaggcg ttcaatcacc cataactgtg agcggtatca atccaacagc
    20041 attaagttcg ggtgtagtag tatctgtaaa tgcaaatatt acacatactt atgatggtga
    20101 catcgaattg cgtttggttg ctccatcagg acaatatgca gtactgagta atcgtagagg
    20161 tggtagtggt gataattata ccaatacaac ttttgcaatg aacggaccaa tggctataac
    20221 cagcggtagt ccgccctata atggaacatt tattcctgaa ggaaacttca acagcctgac
    20281 aggtaatgtt aatggtgttt ggaaactgca tgtaattgac cgtgcatcgg ttgacgtagg
    20341 aacaattcaa aattggacat tgaaaatcaa ttcacaggtt cctacatcaa tatcttatag
    20401 ctggacatct aatccttcag gatttaactc aacaaatcag aatccgatag ctaatccagc
    20461 acaaacaaca acatatacag tttctgcagt tgaaagtact acgggttgtg ccggcacagg
    20521 ttcagtgaca gtgaatgttg caaatcctaa tgttagtgtt tcaggaagca gtacaatctg
    20581 tgcaggaaat tcaacaacac tttctgccag tggagcttca acctattcat ggagtcctgc
    20641 aacaggatta agtgctacta ccggttcaag tgttacagca tcaccgacac aaactactac
    20701 ttattctgtt atcggtatgt tgaatggatg ttcagatacc acacagatta cagtaacggt
    20761 taatcctgta ccaacaaatg taagtgcact tgcaagtcca ggctcattgt gtgttggtga
    20821 tattttgctt ttaaatggtt ttggtactga tgttacttct tggtcatgga caggtcctga
    20881 tggcttcagt tctactcaat cggctccatc aataattaat attcaaccag tacaggccgg
    20941 gaattataca gttaccggtt ctaatgcctg cggcagtgcc tcagcacagg ttattgtgga
    21001 tgttaaacct acaccttacg ctttagttaa tataacatac cctaatggac attgtgcagg
    21061 tagctatgcc ctggtttgtg cctcagatac atcaaacgga gcttatgcac catataccta
    21121 tcattgggat gacaatcaaa caacatcatg tatcaatcaa ctgatacttg caaatccgtt
    21181 gccattacat ggtccgagtg taactattgt taatagtttt gggtgtgcat caacgaatgc
    21241 aacattgtgg agtttgcctt ttggcccggt ggccgattca atacagaatc cacaaacaat
    21301 atgtcaggga ggctcttatg ctataaacgg aaatatttat actgctgctg gaacatacta
    21361 tgataacttt atcaatatat cgggttgtga tagtgtagta attacacagc ttacagttac
    21421 tccttcgctt agtattaatg ttagtggtaa caataccctc tgcgaaggcc agtcaacaac
    21481 attgactgca acaggagcat caacttattc atggagccct tcaacaggtt taaataccac
    21541 tactggtgca acagtgagtg cctcacctac agcaacaact acttatgttg ttacaggaac
    21601 agcaaacgga tgctctaaca gtacaacatt tgtattaaat gtaaatccaa atcctgttat
    21661 tcaaacaagt ggcgatgtag atgtttgcag cggacagttt gtaacgttat cagcgagtgg
    21721 tgcaagcaat tactcatggt ctccatcaac agcattaaat acaacaactg gtacaacggt
    21781 tgttgctaat ccaacttcaa cgattactta cacagttata ggaaccgata atggatgttc
    21841 tgcttcttct tcaattactg taaatgttat tgcacaacca gtcgtaaatg tttctgccag
    21901 cacaacttct atctgtaatg gtcaatcagc tacattaaca gcttcaggtg cagacaccta
    21961 tacttggagc ccatcaacag gattaaatac tacaacagga gcaatcgtaa tagcaacacc
    22021 tgctacaaca accacatata tagtaacagg ttttgttgcc ggtggatgtt ttagtacaaa
    22081 cacaataact atcaatgcta atacaggagg cgcagcacct gctatgcctg gattgatagt
    22141 gggtaataaa aaaccttgtc caacagttaa cgaaaactat tctgtaactc ttgttgccaa
    22201 tgccattgga tatacatgga cagttcctgc aggcgtaact attgtttcag gacaaggaac
    22261 caacaacatt caggtgagtt ttggatctaa cttttcaagc ggtagcgttt cagtagttgc
    22321 taatggcgcc tgtggtgtca gttccaaaag atctctaacc attactaaga atatgccagc
    22381 tacaccatca actattagtg gccctgtaac cggactttgt aatgcagcag gaaactttac
    22441 atctgctaca gtgagtggtg caactactta cacatggtcg gtacctacag gggttagcat
    22501 tgtgagtgga caaggtacca agattgcctc tattaatgtg agcagtagtt tcagtagtgg
    22561 taatgtttgt gttactgcag acaattcttg catgagcagt aaaccacgtt gtgttgcgat
    22621 taagaagact ccttctgttc caacaacttt aacaggacca acaacagttt gtgcaggaca
    22681 acaaaatgtg acttatagta caaatactgt atttggtgca aacaactatc aatggattgt
    22741 tccatcagga tcaataatta cctctggtca aggaaccaca agcatcacag ttaactttgg
    22801 aactaaccct ggcagtgttg gtgttaatgc gtacaatgat tgcggtcatg ccggcagaaa
    22861 agcagtgcat gttgcattta attgccgctt gggtgagatg agtagtattg cttctgatat
    22921 tgaagtatca cccaatccaa cttcaacagg aatagtgaac ctgaaagtta attcagaagt
    22981 taatggtata gcgttaatta aaatgactga tattttaggt aagtctgttt taataaagaa
    23041 cattgcaaat agtattggta caaacaatca taaattagac ttatcaaaat tcaataaggg
    23101 tatttatttg ttaactgttg ataccggtag taaaaaacaa acattaaaag tggtaataca
    23161 ataattttat ctaaaaccga aataaagccc tcctgcggga gggtttttat ttttaaagaa
    23221 tgagaagatt aatattttgt tttttgattt tattttctgt gcaagggtat gcgcaacagg
    23281 tgttcactaa ctattcaaca gcaaattcac cattgcctga aaattcggta agatgtattg
    23341 aagttgatca tataggtaga aagtggattg gaaccgatta tggtcttgct gtttttgatg
    23401 atgtaaactg gaatgtttat ttgcctttca cttctggatt gccagcaatg agtgtgcgtg
    23461 ctattgcatt cgattcactg aacaatgctt ggataggtac gcttggtgga ggagtggcaa
    23521 agttcgatgg gacaaactgg gttgtctatg atgacaccaa cagtccaatc actgaaaatt
    23581 caatcagagc acttgcattt gattcagcag gtgcattgtg gattggaaat caggacggac
    23641 ttgtaaagtt tgacggaacc aactggatag tttattctgc tgcaagcagt atcattcaaa
    23701 atactattgc atctattcat acctcaaaaa acagaaaagt
//
LOCUS       DAOQNP010000046        23382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_748_length_23382_cov_10.565654, whole genome shotgun sequence.
ACCESSION   DAOQNP010000046 DAOQNP010000000
VERSION     DAOQNP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23382
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_748_length_23382_cov_10.565654"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2710)
                     /locus_tag="PKY09_07595"
     CDS             complement(<1..2710)
                     /locus_tag="PKY09_07595"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNB12760.1"
                     /translation="MEKKRTYLGNADPVAIEALYNSYLKDPQSVDQTWQTFFDGFELA
                     RKSQSPDGGNVVSAADASHIEKEFMVLRLISDYRKRGHLFTDTNPVRERRKYQPTLDI
                     QHFGLSESDLPTLFQAGNQIGIGKASLADIITHLRDTYCRSVGVEYKYIRSPEVVKWF
                     EQKMESCKNSLEFTIDDKRKILNKLNEAVAFENFLHVKFAGQKRFSLEGAETTIPALD
                     AVIEFGAKMGIKEFVIGMAHRGRLNVLANILNKGYDEIFTEFEGKAFEDNAFGGDVKY
                     HLGYSGDVETYDGKKVHISLTPNPSHLEAVDALVQGIVRSKIDAQGGTTDDIAPILIH
                     GDAAIAAQGVVYEVIQMSLLKGYSTGGTIHLIINNQVGFTTNYIDARSSTYCTDVAKV
                     TLSPVFHVNGDDVEALIYTVKLAMEYRQKYHRDVFIDILCYRKYGHNEGDEPRFTQPL
                     LYKAIAAHPNPREIYIQKLLSQGEIETNLVKEMEKSFKALLEEKLSIAKKVEKTKVPN
                     FKDGAWSKLRFSTPEDFIQSPDTSVKQNVLLSIAEKITNLPADKKFFNKITRLFGERK
                     EMLKGDGKLDWAMGELLAYGTLLNEGFPVRLSGQDCERGTFSHRHAVVRVDDSEEQFT
                     PLNHISDKQARFYIYNSLLSEYGVLGFEYGYAFTSPNSLVLWEAQFGDFGNGAQIIID
                     QYLSSAEDKWNRMNGLVMLLPHGYEGQGAEHSSARMERFLTLSAEYNMVVTNCTTPAN
                     FFHALRRQLKWPFRKPLIVFTPKSLLRHPLCVSTISDFTDKKFSEVIDDTVKAADVSR
                     VILCTGKVYYDLLQHRTVEKISDVALIRLEQLYPFPHQQVEKILKKYSKADDIIWLQE
                     EPDNMGAWGFIKRMMPELNIRLIARVESASPATGSHSAHDEEQQQLL"
     gene            complement(2810..3529)
                     /gene="recO"
                     /locus_tag="PKY09_07600"
     CDS             complement(2810..3529)
                     /gene="recO"
                     /locus_tag="PKY09_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNB12761.1"
                     /translation="MSALHNTDGIMLHLSPYGDSSVIAKIYTEKFGMQTYLVNGVRSS
                     KAKNKSVFFQPLSLLQLVVYHRPDKGLQRISSLAFANRFVSIPFEITKSAQSIFIAEL
                     LYRTIREEEANHALFSFLFDAIVQLDNTISANPDFHLLFMIRLSAFLGFGPTDNYEEQ
                     LCFDMKEGCFSTKNSSHLYVIDASTSLLLHQCLSSSIKELQLNHKQRTDLLNVMLQYY
                     QLHLPDGFRLQSVDILTELFN"
     gene            3712..4146
                     /locus_tag="PKY09_07605"
     CDS             3712..4146
                     /locus_tag="PKY09_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB12762.1"
                     /translation="MFSKACEHGIKAIIYIATQSMDGKRVKIGDVVENSGSPEAFTAK
                     VLGSLTKYHIVNSYTGPNGGFFIDVKDMKKIKMSDIVNAIDGDSLYNGCGMGLSECSK
                     TQPCPMHDKFIKVRKEIKEMLTTTSVYDLALGLKSGKTVLIR"
     gene            4200..4433
                     /locus_tag="PKY09_07610"
     CDS             4200..4433
                     /locus_tag="PKY09_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNB12763.1"
                     /translation="MESKEIWLTSLITKTPQEGRELAIKMARKSIAAIQTDPEVRKKL
                     RDDYANDTKQLIASANVVALEFQTVAAANNYWK"
     gene            4513..4899
                     /locus_tag="PKY09_07615"
     CDS             4513..4899
                     /locus_tag="PKY09_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNB12764.1"
                     /translation="MRDIENIKDIQLLVDSFYSKIRNDEQLGHIFNNIIKDRWPEHLE
                     KMYRFWQTILLDDQTYFGSPFVPHAKMPVDKTHFEQWIKLFSETVDENFVGKKAEQAK
                     WQGQRMADMFHYKIEYIKNSSTTPLQ"
     gene            4896..5351
                     /locus_tag="PKY09_07620"
     CDS             4896..5351
                     /locus_tag="PKY09_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12765.1"
                     /translation="MIVVYLPIALIASTLWIGFVSAISFMEAWLKFRAPGITIPLGLG
                     IGRLVFNVLNKIEWVLATLIICNLILTKEQFINPIKGGHGMFYLIPLLILLFQTLWLL
                     PQLDARAQMQINGKQVSPSKLHIYYAGLEVVKVVFLATFILNLFKNITT"
     gene            5348..6280
                     /locus_tag="PKY09_07625"
     CDS             5348..6280
                     /locus_tag="PKY09_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNB12766.1"
                     /translation="MNNEKLISDIENKYQQTGENSETYLKGLLHAKPITYWDYVEVDT
                     LLSLQRPRTNFKDEEIFVMYHQVTELVLKMMVHEIKQMVFDPFAEHIWIDKINRLNRY
                     TSMLITSFDVMKYGMNYDDYNTFRSTLAPASGFQSAQFRFIEIYLTRLENLMNEEGRK
                     RLGNNPSIEDYFEHIYWKDAGMDRKTNKKSMTLKLFEEKYLESFIALAKKVQGNTIED
                     QVMRMGNCSDALKSKLKEFDHMYNVAWPIVHLETAQHYLDLKGENKAATGGSEWKKYL
                     HPKYQQRKFFPSLWTKEEIANWGSNEFQTTTNKK"
     gene            6451..7221
                     /locus_tag="PKY09_07630"
     CDS             6451..7221
                     /locus_tag="PKY09_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014781489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB12767.1"
                     /translation="MDTKDIHKAHGHWILAKMGKKVLRPGGKELTKKLIDALHFNNSD
                     NVVEFAPGMGFTASLTLAYKLHSYVGVDADEDIVRMLSKKFQSSQASFILGNASQTNL
                     ESESKDKVYGEAMLTMHADHRKSEIIREAHRILKKGGWYAIHELGLTDVDETLKSKIQ
                     RDLALSIKVNARPLTEVEWKHLLEQEGFAVKKVMTNGMHLLEMNRIIDDEGLLRATKI
                     GWNILTHPKARKRILEMRKVFKQYQNHMNAIAIVAEKL"
     gene            7232..7960
                     /gene="ric"
                     /locus_tag="PKY09_07635"
     CDS             7232..7960
                     /gene="ric"
                     /locus_tag="PKY09_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNB12768.1"
                     /translation="MHITKDTIVGDLVAKDYRTATVFKQNGIDFCCNGNRTLEEVCKQ
                     KEMSVSNIIGELTQASQQTSAAQSDFNSWPLDLLADYIEKKHHRYVEEKTQEIKPYLD
                     KICKVHGDHHPELHEINRLFNASTGELAMHMKKEELILFPAVRRMVKSQMQKTTPEKP
                     QFGSINNPIKMMMHEHNNEGERFRQIEELSSKYNPPQDACNTYRVTFAMLKEFEDDLH
                     LHIHLENNILFPKALELEKQIYHE"
     gene            7953..8417
                     /locus_tag="PKY09_07640"
     CDS             7953..8417
                     /locus_tag="PKY09_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB12769.1"
                     /translation="MSEPIKRNEALKPISRDHHHALLLGWKIRQGIKNKIPAERIKKY
                     VDWFWSSYLSNHFELEEKNLYPVLGAEHASIKKAMQHHEKLRNLFLSKEQTIQQLDEL
                     QNKLDRHIRFEERELFNEIQNAATPKQLQEMAAVHNESFHDNYNDEFWVTKK"
     gene            8495..9766
                     /locus_tag="PKY09_07645"
     CDS             8495..9766
                     /locus_tag="PKY09_07645"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB12770.1"
                     /translation="MNIPDLKIPRVVIVGCGFGGLQLAKSLRKARVQIVMFDTNNYHT
                     FQPLLYQVATSGLEPDSIAYPIRKIFKKQKNFLFRWGKVENIIPEKNIVQTTVGELTY
                     DYLVIATGTTTNYFGNADIAQHAMPMKSVIEAINLRSLILQNFEQALLTQSLKEQEAL
                     MTFTVVGGGPTGVELAGALCELKKHVLPNDYPELDFRKMRVLLIEGTNTVLQAMSKVN
                     QHRALKYLDELGCEVWLETQVSSYNSEEVVFKNGKVLPSKNLIWAAGIKGVTISGLQL
                     DSINNRGRYIVDEYCKIKGCENIYAIGDVASMPTTNFANGHPQVAPVAIQMAQLLAEN
                     IQQELRHKPLKKFNYFDKGTMATVGRNRAVTESFNIRFGGFAGWLVWMALHLMLLVGF
                     RNKVVVFINWLWNYINYDRNIRLIIPPYKRK"
     gene            complement(9846..11150)
                     /gene="sufD"
                     /locus_tag="PKY09_07650"
     CDS             complement(9846..11150)
                     /gene="sufD"
                     /locus_tag="PKY09_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNB12771.1"
                     /translation="MNSSVDTLSVTNETSIAEENIPVDGLQKLRLAALNKFLKNGFPD
                     SKHEEWKYLNLKPISETKYNHCMVASPLKASDLEQFYDAGSDTVNLTFINGRYCSEFS
                     GHQSHKHPFSIYSLESKEAELYLETLRNSEDEPFHALNTAFHQGGIMIHVPDNCRMPM
                     AIHLLYVNDSQHEAVAAHPKCIVVAGKNSAFQVIATYHSLNNFPSLTNTVTEFLIGEG
                     AVVEFDIKQNENSNANHINRVYARVQRKAVFDIFTVTMGGALVRNNLEIILSEPNACA
                     HLNGLTVADGYQVVDNHTVVDHASPNCESNQLYKNILDDHSQGVFNGKIFVRKDAQKT
                     NAFQSSKNVLLSNTAVMNAKPQLEIYADDVKCSHGATTGQLDEDALFYFRSRGIGERD
                     ARALLNYTFASDVIEKINNDALKSNLLKLLSQKLNTAVEFGE"
     gene            complement(11162..11914)
                     /gene="sufC"
                     /locus_tag="PKY09_07655"
     CDS             complement(11162..11914)
                     /gene="sufC"
                     /locus_tag="PKY09_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNB12772.1"
                     /translation="MLNIKNLHASVEGKEILNGLNLQVNPGEVHAIMGPNGSGKSTLA
                     SVLAGREAYEVNEGEVLYHGESLIELSPEDRARKGIFLAFQYPIEIPGVSNANFLKTA
                     VNEVRKSKGLEALDSKDFLKLMKDKMQLVKMDKSMTQRSVNEGFSGGEKKRNEIFQMA
                     MLEPELCVLDETDSGLDIDALRIVSDGINALRDGKRSFIVITHYQRLLDYIVPDFVHV
                     LYKGKIVKSGPKELALELEEKGYDWIKEEVEI"
     gene            complement(11962..13407)
                     /gene="sufB"
                     /locus_tag="PKY09_07660"
     CDS             complement(11962..13407)
                     /gene="sufB"
                     /locus_tag="PKY09_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNB12773.1"
                     /translation="MSEDLKIIDEVTSGEYKWGFVSNIESENAPKGLSEDIVRFISAK
                     KKEPEWMLEFRLKAFRHWQKMQEPQWANVNYPKVDFQDIIYYSAPKPKKQLNSLDELD
                     PEIKATFDKLGISLLEQKRLAGVAVDAVIDSVSVKTTFRQELSKLGIIFSSFSEAVQE
                     HPELVKKYMGSVVPYTDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINSAGTG
                     QFERTLIIADEGAYVSYLEGCTAPKRDENQLHAAVVEIYAHTDAQVKYSTVQNWYPGD
                     KDGRGGIFNFVTKRGLCAGERSKISWTQVETGSAVTWKYPSVILKGDYSTGEFYSVAL
                     TNNYQQADTGTKMIHIGKHTRSTIISKGISGGRSNNSYRGLVRIGKQAEKARNFSQCD
                     SLLLGDKCGAHTFPYLECANPTAKVEHEATTSKVGEDQIFYCNQRGISTEDAIGLIVN
                     GYCKEVFNQLPMEFAVEAQKLLAVSLEGSVG"
     gene            complement(13481..13807)
                     /locus_tag="PKY09_07665"
     CDS             complement(13481..13807)
                     /locus_tag="PKY09_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNB12774.1"
                     /translation="MITVTEKAKSKALSLMQAENKPADAFIRVGVEGGGCSGLTYMLV
                     FDTELKPEDKVFEDKGIKIVCDRKSFLYLVGTELDYTDGLNGKGFQFNNPNASRTCGC
                     GESFAV"
     gene            13946..14995
                     /gene="thiL"
                     /locus_tag="PKY09_07670"
     CDS             13946..14995
                     /gene="thiL"
                     /locus_tag="PKY09_07670"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNB12775.1"
                     /translation="MFENANNRTELSSLGEFGLIRHLQKAVEINNKETLKGIGDDAAV
                     ISLDSDENVQLITTDMLLEGVHFDLVYCPLKHLGYKSVVVNLSDIYAMNGMPKQIVVG
                     IGLSNRFSLEAIEELYSGMLLACKHYGIDLIGGDTTSSRSGLVISITAIGTAKKSDVV
                     YRNGAQENDLICVSGDLGAAYLGLQLLNREKKVFLDSPGVQPDFQGNDYLLERQLKPE
                     ARKEIVEILKKTGVKPTAMIDISDGLASEIMHICTQSDKGCTIYEAKVPIDEKTYNLA
                     REFHLDPTVCAMNGGEDYELLFTINQKDFEKINSAADISIIGHITAKEDGYYMLDKSD
                     RKVALKAQGWDALKS"
     gene            15007..15786
                     /locus_tag="PKY09_07675"
     CDS             15007..15786
                     /locus_tag="PKY09_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNB12776.1"
                     /translation="MSQKNYRILCWNVNGIRAIWKKGFPEWLTKEMPDVLCLQETKAQ
                     PEQLDDEIKNFSGYKSYFFSAQKKGYSGVAIYTKHEPLKVRYGLDDDTFDVEGRCIEM
                     EFEDFVLYTAYFPNGGRGPERVKYKLDFYDSLFQRAENMRKKNKNIVICGDYNTAHKE
                     IDLARPKENSKTTGFLPIEREWIDKIIDMKYVDIFREFNKEAGQYTYWDQITRARDRN
                     VGWRIDYYMISEELRKYVKSAPIHMNVMGSDHCPIELNLSF"
     gene            complement(15813..16796)
                     /locus_tag="PKY09_07680"
     CDS             complement(15813..16796)
                     /locus_tag="PKY09_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNB12777.1"
                     /translation="MQKDPYIILGVSYSATPEEIKRAYRKKAFELHPDRNSNDQAEEL
                     FRELNEAYALLTDTEKRRNFDKQYQSYSRSNADQFNHYFEMYVSSNNVKVCEEFELTF
                     TYTGEGRFMQKPDLRDFYIAGKPFVSFQDVYRSGKNARETTIRYVLAAKRTGNFTINR
                     AAIKLFGKTFFTTSQFIQVAPGKCFFSPDKFADGKPLQVSLWYNAEKGGTNRKYLVNT
                     KHNIYIPRSHYAQVYHTIGRVLKLFFALWGLILAIKIDRNVVIGFIIGSAYGAMMTYL
                     LYYFVKVKPVFMADRKYSLVNSYLQNGYQPYPPHARNLISRAIMFVNKLFL"
     gene            16888..18744
                     /locus_tag="PKY09_07685"
     CDS             16888..18744
                     /locus_tag="PKY09_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB12778.1"
                     /translation="MKNYFKILRFVKPYWGYALLNASCNILSVLFSFFSLTLIAPFLD
                     LLFLKDDNFYLEKMAAGKPEFHLSTKAIVDSFYYYLTEMITDPNRGKQYALMFICIAV
                     AVMFFLKNLFRYLGQFFISPIRNGVVKDIRTEVHKKIMRLPLSWFGNERKGDIITRVT
                     SDVHEVEWSVMSSLEVVFRDPFNIFLFLGMMIFISAKLTVFVFILLPIVGFIIGRIGK
                     SLKRTSAKSKEKLGLLISIIEETLSGLRIIKAFNAENFMSEKFSKVNEQYNTIAIRAL
                     RKTDLSSPLSEFLGTVTLMIVMYFGGMLVLEKDALLSASAFITYIALFSQIIPPAKSF
                     TQAFYNIQKGLASADRIFAVLEAEESIKDHQSAETIKSFNDKIEYQNISFAYRKGDEG
                     YVLKNVSFTIDKGKTIALVGQSGSGKTTLADLLPRYYDVDAGALLIDGTDIRKLQLHT
                     IRSLLGVVSQEPILFNDTIYNNIVFGMSNATEKMIEEAARVANAHEFIMQLPEGYQTN
                     IGDRGSKLSGGQRQRISIARAVLKNPPILILDEATSALDTESEKLVQDALTSLMKNRT
                     VLVIAHRLSTIQHADEIIVLQKGEIVQRGTHAQLANVEGVYKKLYELQGLSS"
     gene            complement(18753..19514)
                     /locus_tag="PKY09_07690"
     CDS             complement(18753..19514)
                     /locus_tag="PKY09_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB12779.1"
                     /translation="MKIVIFEDEEPAARRLIKLLNEVSTDVEIVSVIDSVRSGKEFMK
                     QNKSFDLIISDIRLADGLSFEIFKSHTVKLPVIFSTAYDEYAIEAFRNNGIDYLLKPV
                     KREELKTAIQKYENWFSDSTSGVNYDVLLQTLLQEKKGFQKRIVIRFADTIKTVEIEE
                     AAYFFTENKISNLCTFNGESFIIDYNLDELELILDPAVFFRINRQFIVNFKAIQKMNV
                     VSKSRVKLTLHPSTTQDTIVSTERSGVFKQWLAGK"
     gene            complement(19514..22387)
                     /locus_tag="PKY09_07695"
     CDS             complement(19514..22387)
                     /locus_tag="PKY09_07695"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB12780.1"
                     /translation="MKRIIVFLFLIVQTVNGQNSFYKQISLNRDSPNLTVNALLFCKN
                     GFLWAGTSDGLYRSDGNNHHLLAVNDTIKSNVTALFEDATGVIWVGTKDGKIGFVEDD
                     ILKIIKVFALKTAVAITSINDDGNGIKWFTTAGNGVYYFKDKKFYHIGTEDGLSDDFC
                     YTSALDKSGNIWIGTDQGLSLCTSNKEKKVLRKLQAADGLCDNIVKKIIIENSKLLLG
                     TQENGLCIFDIKQNKFLKQQEDSVWKYGAVNDIILNRLTHEIWMATESGGLLYCRRPE
                     ENIFSFKQQSDKFFSTVYSVAIDHQNNVWLATDRGLYRSHGQWLTFLDQYSGVDIKNV
                     HEVTFLDSNQMVMSIKNKVMLISFNTSTVKEWTILPSEKNIDVSGLYIDKEKYIWVGT
                     LGSGVFRINPSKNEIKKIEVPFDYSSILYINGNEKNVWLGTFGGAVLIETKNLNTDNL
                     QSKIRSFSHESLLGNYYVYCVYRDVKGRFWFGTDQNGIVLLDRDKFVTPFKGKFKGKT
                     VYSITGDKNDAIWFSVADEGIVCLNGDSYKLYNRSNGIRDLNITSIAVLDDSSIAMVN
                     KRGIDILNYKTGSVMYLGTENNLDNLNPDINSVAKDSLGRIWFGSAKGMVVLDPKLLK
                     QNTVPQLSKLNMIQPIFRNYSGMMPVFRHNENTISFEYAATWFTNPGRILYQYKLDGL
                     NSDWIETKDVKQVFPNLPSGKYVFRVRTSIDPQFTFNNERIFSFEIKKPFWSEEWFYI
                     TSALFTILLVYFFIRLRDARMKKLQQLKNDSIRFQFETLRNQVNPHFLFNSFNTLIDI
                     IEHDREHAVEYVENLSAFFRNIVTYKDKDTIKLSEEIKIVKAFYFIQQKRFGSNLQLL
                     VDEHSLYDDRYVVPMVLQLLLENAIKHNEISKEKNLKVKVSFSDSRIIISNNTNPKMN
                     KDESTGTGLRNIIARYRLLTDKTVQIESSQEYFVVSLPLIIRE"
     gene            complement(22384..22974)
                     /locus_tag="PKY09_07700"
     CDS             complement(22384..22974)
                     /locus_tag="PKY09_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNB12781.1"
                     /translation="MVISIEINIFVLMKQGGVLTILFFILIFSVKVNGQLVYYSTNGE
                     VFFRSEAKLELIDAKSQKLEAMIDFQKKIFEFLIPINSFKGFNAELQREHFLDRYMES
                     DKFPVATFTGKIIEDVDISKPNEKTIRAKGKLIIHGVEQERIIKSKISVQGNGITILS
                     VFSVPLKDHKIKVPRVVQDKIANEVLVQVKATLRRK"
     gene            23018..>23382
                     /locus_tag="PKY09_07705"
     CDS             23018..>23382
                     /locus_tag="PKY09_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNB12782.1"
                     /translation="MYSEQSHKFHGQLFKYATESVVVTNSKGIIVTVNPSALKLFGYT
                     ENEIIGQKIEKLMASRFEASHQLHREGFNKNPHNRAMGIGLDLHAMRKDGSEFPVEIS
                     LSPFSLNDESYVMAFIIDIT"
BASE COUNT         7626 a         4288 c         4083 g         7385 t
ORIGIN      
        1 taagcaactg ttgttgttcc tcatcatgag cactgtgaga tccggttgca gggctggcac
       61 tttcaacacg cgcaatgagt ctgatgttta actcaggcat cattcgtttt ataaaacccc
      121 atgcacccat attatcaggt tcttcctgta gccagatgat atcatctgct tttgaatatt
      181 tctttaaaat cttttcaacc tgctgatgcg ggaaaggata aagttgttca agtcttatta
      241 gtgctacatc agaaattttc tccactgtgc gatgttgtaa taagtcgtaa tacactttac
      301 ctgtacataa aattacacgc gatacgtcag cagccttaac agtatcatca atcacttcac
      361 tgaatttttt atctgtaaaa tcacttatag tagaaacaca gagaggatga cgaagtaaac
      421 tctttggtgt aaacacaatc aaaggctttc taaaaggcca tttaagctga cgtcttaaag
      481 cgtggaaaaa attagcaggg gtagtacagt tggtaacaac catattgtat tctgcactta
      541 gtgtaagaaa tcgctccatc ctggcactac tatgctcggc accttgtcct tcataaccat
      601 gaggcagcaa catgaccaat ccgttcatgc gattccattt atcttctgca ctgctcagat
      661 actgatcaat aattatctgt gcaccatttc cgaaatcgcc aaactgtgct tcccataaaa
      721 ccaaactgtt gggtgaagta aatgcataac catattcaaa tcccagaact ccatactccg
      781 acaacagcga attgtaaatg taaaaccgtg cttgtttgtc tgaaatgtga ttgagtggtg
      841 taaattgttc ttccgagtca tcaacacgaa caacagcatg gcggtgcgag aatgttccgc
      901 gttcacaatc ttgtccgcta agacgaacag gaaacccttc attaagtaaa gttccgtaag
      961 ctaaaagctc acccattgcc caatctaact taccatcacc ttttaacatt tcttttcttt
     1021 caccgaaaag tcttgttatc ttattaaaaa actttttgtc tgcaggaaga ttggtaatct
     1081 tttccgcgat tgacaacaac acattttgtt taacagaagt atcaggagat tgaataaagt
     1141 cttcaggtgt tgaaaaacga agtttactcc atgcaccatc tttaaagtta ggaacttttg
     1201 ttttttcaac tttcttcgca atgcttaatt tttcttcaag caatgctttg aatgatttct
     1261 ccatttcctt caccaggttc gtttctatct caccttgtga aagtagtttc tgaatataaa
     1321 tttctcttgg attgggatgt gcagctatgg ctttatataa aagtggttgt gtgaaacgtg
     1381 gttcatcgcc ttcgttgtgt ccatattttc tgtaacaaag aatatcaata aaaacatcac
     1441 gatgatactt ctgacgatat tccattgcta atttcactgt atagatgagt gcttcaacat
     1501 catcgccatt gacatgaaac accggtgata aagtaacttt tgcaacatcg gtacaatagg
     1561 tgcttgaccg tgcatcaata taattcgttg taaaacctac ctgattgttt atgataagat
     1621 gtatagttcc tccggtagaa tatcctttga gtaacgacat ctgaataact tcataaacta
     1681 caccttgtgc tgcaatggca gcatcaccat gaatcagtat aggtgcaata tcatctgttg
     1741 ttccaccctg tgcatcaatt tttgaacgca caataccttg taccaatgca tctactgctt
     1801 cgaggtgtga aggatttggt gttaaagaaa tatgtacttt cttgccatca taggtttcaa
     1861 catctcccga atagccaaga tgatatttta catcaccgcc aaaggcatta tcttcaaaag
     1921 cttttccttc aaactcagtg aaaatttcat cgtagccttt attcaaaata ttagcaagca
     1981 cattgagcct gcctctgtgc gccattccga taacaaattc tttgataccc atttttgcac
     2041 caaactctat tactgcatca agagcaggaa ttgttgtttc agcaccttca agagaaaaac
     2101 gtttttgccc tgcaaacttc acatgcagga aattttcaaa ggcaacagct tcgttcagct
     2161 tattcagaat ttttctttta tcgtcaatgg taaattcaag gctgtttttg caactttcca
     2221 ttttctgttc aaaccactta acaacttcag ggcttctgat atatttatat tccacaccaa
     2281 cacttcggca atacgtgtca cgaagatgtg taataatatc tgctaatgat gctttaccta
     2341 ttcctatttg attccctgcc tgaaaaagcg taggaagatc actctctgac aatccaaaat
     2401 gctgaatatc taatgtgggt tgatattttc tgcgttcgcg aacaggattg gtatctgtaa
     2461 aaagatgtcc acgcttacga tagtcgctga ttagtcgcaa aaccataaac tccttttcga
     2521 tatgtgatgc atctgctgcg gaaacaacat ttccgccatc aggcgactgg ctttttcttg
     2581 ccagttcaaa tccgtcaaaa aaagtctgcc aggtttgatc tactgactgc gggtctttga
     2641 gatatgagtt ataaagcgct tctattgcta ctgggtcagc attaccgagg taagtacgtt
     2701 tcttttccac tttaaatttt aactgaattc agcgcacaaa ggtagtcaat ttgccggtgt
     2761 aaaaactgat tttagttagc tgaataacca atctccatat aaaaactcat caattaaaca
     2821 gttcagttag tatatcaacg ctttgcaaac gaaaaccatc aggaagatgc agttgatagt
     2881 attgtaacat aacatttagt aaatctgttc gctgtttatg atttaattgc agttctttta
     2941 ttgaagaact aagacattga tgcaataaca aagaagtaga tgcgtcaata acataaaggt
     3001 gtgaagaatt ttttgtactg aaacagcctt ctttcatatc gaaacatagc tgttcctcat
     3061 aattatctgt aggtccgaat cccagaaaag ccgaaagtct gatcataaag agaagatgaa
     3121 aatcaggatt tgcagatata gtattatcga gctgaacgat ggcatcaaac aaaaaactga
     3181 aaagggcatg attggcttct tcttcacgaa tggttcggta aagcaattca gcaataaaaa
     3241 ttgactgtgc gcttttagta atttcgaagg gtatagaaac aaatctgttg gcaaaagcca
     3301 gactacttat gcgttgcaat cccttatcag ggcgatgata aacaacaagc tgaagcaaac
     3361 ttaatggctg aaaaaaaaca cttttgtttt ttgcttttga agaacgcacg ccattgacta
     3421 agtaggtttg catgccaaac ttttctgtat aaattttagc aataacggat gaatcgccat
     3481 agggcgacag gtgtaacata attccgtctg tgttatgcaa ggcagacatt ctacttttct
     3541 aatctgaata ttttattgca aatttatgac atgattttga cgattcaaca taaaaagaaa
     3601 acagttttaa cagttattga acacattgaa aactgcctct aaaattaatt tacgatattt
     3661 ttgtcgtaaa ttaaaaaact tcctactttt gtagcccgaa aagacgcaac aatgttttct
     3721 aaagcatgcg agcatggcat aaaagccata atatatatag caacacaatc catggatgga
     3781 aagcgtgtaa aaattggtga tgttgtagaa aactcaggct caccggaagc ttttactgca
     3841 aaagttctgg gatcattaac caaatatcat atcgttaatt catacaccgg accaaacgga
     3901 ggttttttta tagatgtgaa ggatatgaaa aaaataaaaa tgagcgacat tgttaatgct
     3961 attgatggcg actcattata taatggctgt ggtatggggt tgagtgaatg cagcaaaaca
     4021 cagccatgcc ctatgcacga taaatttatt aaagtcagaa aagaaataaa agaaatgtta
     4081 actacaactt ctgtttacga tctggcatta ggacttaagt cgggcaaaac agtattaata
     4141 cgataaataa ttccgcacga acaattattc agtacataaa caattaaaat aaaaacacaa
     4201 tggaaagtaa agaaatatgg ttaacatcat taattacaaa aacacctcag gaaggaagag
     4261 agttggcaat taaaatggca agaaaatcaa ttgcagcaat acaaactgat cctgaagtaa
     4321 gaaaaaaatt gcgtgatgat tatgccaacg acacaaaaca actgatagct tctgctaatg
     4381 tagtagcatt agaatttcag actgtggcag cagcaaataa ttattggaaa taattcatca
     4441 tagtttacta ataaaaaaca gaactacagg atttacatta aagttaaaac aaaatatact
     4501 tcagcaatag agatgagaga tattgaaaac ataaaagata tacaattatt ggtagatagc
     4561 ttctatagta aaatccgtaa tgatgaacaa ttaggtcata tctttaataa cataataaaa
     4621 gacagatggc ctgaacatct tgaaaagatg tatcgtttct ggcaaacaat tttgttagac
     4681 gatcaaactt attttggtag tccttttgtt ccacatgcta aaatgccggt tgataaaact
     4741 catttcgagc aatggataaa attattctcc gaaacagttg acgaaaactt tgttggcaaa
     4801 aaagctgagc aggcaaaatg gcaaggtcag cgtatggctg atatgtttca ttacaagatt
     4861 gaatatatta aaaacagttc cactactcca ctacaatgat agttgtttat ttacccatag
     4921 ctttgattgc atccacatta tggattggct ttgtaagtgc aataagtttt atggaagcct
     4981 ggcttaaatt cagagcaccg ggaattacca taccattagg tttaggcatt ggaagattgg
     5041 tatttaatgt gctaaataaa attgaatggg ttcttgcaac attaatcatc tgcaatctta
     5101 ttttaacaaa agaacaattt atcaatccga ttaaaggtgg acatgggatg ttttatctca
     5161 tccctctgct tattctatta tttcagacac tttggctttt accacaactt gatgcccgtg
     5221 cgcaaatgca gattaacggc aagcaggttt caccatcgaa gcttcacatt tattatgcag
     5281 gacttgaagt ggttaaagtg gtatttttag caacatttat tttaaatctc tttaaaaaca
     5341 taacaacatg aacaatgaaa aactaatcag tgatattgag aataaatatc agcaaacagg
     5401 agaaaattca gaaacctatc tgaaaggact tttacatgca aaacccatta cctattggga
     5461 ctatgtagaa gttgatactc ttttatcact acaaagaccg agaacgaatt tcaaagacga
     5521 agaaattttt gtgatgtatc atcaggttac tgagttggta cttaaaatga tggtacatga
     5581 aataaaacaa atggtttttg atccttttgc tgaacatatt tggattgata aaatcaaccg
     5641 tctgaacaga tacacatcta tgctcattac atcatttgat gttatgaaat atggaatgaa
     5701 ctatgatgat tacaatactt tcagaagcac actggctcct gcaagtggat ttcaatcggc
     5761 acaattccgt tttattgaaa tttacctgac acgattggaa aatttaatga atgaagaagg
     5821 aagaaagaga ttaggaaaca atccatcaat agaagattat tttgaacaca tctactggaa
     5881 agatgcaggc atggatcgta agacaaacaa aaaatcaatg accttgaaac tgtttgaaga
     5941 aaaatattta gaaagcttca ttgcacttgc aaaaaaagtt caaggcaaca ctattgaaga
     6001 tcaagtgatg cgaatgggta attgctctga tgcacttaaa tcaaagctaa aagaatttga
     6061 tcacatgtat aatgttgcat ggcctattgt tcaccttgaa acagcacaac actatcttga
     6121 tttaaaagga gaaaataaag ctgctacagg tggaagcgaa tggaaaaaat atttacatcc
     6181 gaaatatcag caaagaaaat tcttcccttc attatggaca aaagaagaaa ttgcaaattg
     6241 gggaagtaac gaatttcaaa ctacaacaaa taaaaaataa taatatcacc gttttgactc
     6301 tgaaagcaac ttgtttgctt taatatctga aaaagattta tccctttcag ataacctctc
     6361 actcaacacc tgaatagtca aatagattca gaacttgtgg ttataatttg tgaaacatta
     6421 aaaagagaaa tcacaataaa ataaataatc atggatacta aagatataca taaagctcat
     6481 ggccactgga tattggccaa aatgggcaaa aaggtgttaa gaccgggagg taaagaactt
     6541 actaaaaaat taattgatgc cttacatttt aacaattctg ataatgtagt ggagtttgca
     6601 cccggaatgg gttttacggc atcattgact cttgcatata aattacattc gtatgtaggt
     6661 gtagatgctg atgaagatat agtgagaatg ttaagtaaaa aatttcaaag ctcgcaagca
     6721 tcttttattc tcggaaatgc atcgcaaaca aatctcgaaa gcgaatcaaa ggataaagtt
     6781 tatggagaag caatgctaac catgcatgcc gatcatagaa aatcagagat tatcagagaa
     6841 gcacatcgca ttctcaaaaa aggaggttgg tatgccattc atgaattagg gttaaccgat
     6901 gttgatgaaa ctttaaaaag taaaattcaa agagatttgg ctttatcaat aaaggtcaat
     6961 gcacgtcctc tcactgaagt cgaatggaaa catttgctgg agcaagaagg ttttgcagta
     7021 aagaaagtca tgactaacgg catgcacctt cttgaaatga acaggattat agatgatgaa
     7081 ggtttgttga gagccacaaa aattggctgg aacatcctca ctcatccaaa agcaagaaaa
     7141 agaatacttg aaatgagaaa agtattcaag caatatcaaa atcacatgaa tgcaattgca
     7201 atagttgctg aaaaacttta aaaataaaat tatgcatatc acaaaagata caattgttgg
     7261 cgatttggtt gccaaagatt atagaacagc cacagtattt aaacaaaatg gtatagattt
     7321 ttgttgtaat ggtaaccgta cacttgaaga agtttgtaaa caaaaagaga tgtcagtatc
     7381 aaacataatt ggtgagttaa ctcaggcttc gcagcaaaca tcagcagcac aatcagattt
     7441 taactcatgg ccattagatt tactagcaga ctatattgag aaaaagcacc atcgttatgt
     7501 agaagaaaaa actcaggaga taaaacccta cttagataaa atatgcaaag ttcatggaga
     7561 tcatcatcct gagttgcatg aaatcaatcg gctttttaat gcttctaccg gtgagttagc
     7621 catgcacatg aaaaaagaag aattgatttt atttccggca gtaagaagaa tggtgaaatc
     7681 tcaaatgcaa aaaacaacac cggaaaagcc acagttcgga tcaataaata atccgattaa
     7741 aatgatgatg catgaacaca ataatgaagg tgaacgtttc agacaaattg aagaattgag
     7801 cagcaagtac aatccaccac aggatgcatg caacacctac agagtgacgt ttgccatgct
     7861 caaagaattt gaagatgatt tgcatttgca cattcatctt gaaaataaca tccttttccc
     7921 aaaggcatta gaacttgaaa aacaaattta tcatgagtga gccgattaaa agaaatgaag
     7981 cacttaagcc aataagtcgc gatcatcatc acgccctctt gctgggctgg aaaatcagac
     8041 agggaataaa aaataaaatt cctgcagaaa gaattaaaaa atatgttgat tggttttggt
     8101 caagctacct ttcaaatcat tttgagttgg aagaaaaaaa cttgtatcct gtattaggtg
     8161 ctgagcatgc aagcattaaa aaagctatgc agcatcatga aaagcttaga aatctttttt
     8221 taagtaagga gcagactatt caacaattgg atgaattaca aaacaaactt gacagacata
     8281 ttcgttttga agaaagagag ttgtttaatg aaattcagaa tgcagcaaca ccaaaacaat
     8341 tacaggaaat ggctgctgtg cacaatgaat cttttcatga caactacaat gatgagtttt
     8401 gggtaactaa aaaatagttt ttcgctattc agaccaaaga cactaaatca tggcgtgatg
     8461 tttaactcaa catctgtttt taaaataaat ttctatgaat attcctgatc tgaaaatccc
     8521 tcgtgtagtt attgtaggat gtggttttgg tggattacaa ttagcaaaat cgcttcgtaa
     8581 agcacgtgtg caaatagtga tgtttgatac taacaattat cacacttttc aaccgttact
     8641 ttatcaggtt gccacctcgg gtttagagcc tgattctatt gcttacccta tcagaaaaat
     8701 ttttaagaaa caaaagaatt ttctttttcg ctggggaaag gttgaaaata ttattcctga
     8761 aaaaaatatt gttcaaacca ctgttgggga actaacatac gattatctgg taattgctac
     8821 cggaacaaca acaaattatt ttggtaatgc tgacattgcg caacatgcca tgcccatgaa
     8881 atctgtaatt gaagctatca atctacgcag tttaattctt caaaattttg aacaggcatt
     8941 gctcacacaa agtttaaaag aacaagaagc gcttatgaca tttactgttg taggtggcgg
     9001 ccccacaggt gttgaacttg caggtgcatt atgtgaatta aaaaaacatg ttttgccaaa
     9061 cgattatcct gaacttgatt tcagaaaaat gcgtgtgttg ctaattgaag gcactaacac
     9121 tgttctgcag gcaatgagca aagtaaacca gcatcgtgca ttaaaatact tagatgaatt
     9181 aggttgtgaa gtctggttag aaacacaggt gtcgtcttat aatagtgaag aagttgtttt
     9241 taaaaacggc aaagtgttgc caagtaaaaa tttgatttgg gctgccggaa ttaaaggtgt
     9301 tactatttca ggattacagt tggattccat caataatcgg ggacggtata ttgttgacga
     9361 atattgcaag ataaaaggat gtgaaaatat ttatgcaatt ggtgatgttg ccagtatgcc
     9421 tacaacaaac tttgctaacg gacatccaca ggtggcgcct gttgccattc agatggcaca
     9481 actgcttgct gaaaacattc aacaggaact aagacacaag ccacttaaaa aattcaacta
     9541 ttttgacaaa ggcactatgg caactgtcgg aagaaatcgt gcagtaactg aatctttcaa
     9601 tatccgtttt ggtggttttg cagggtggtt ggtgtggatg gctttacatt taatgctcct
     9661 tgtcggcttt cgcaacaagg ttgtggtatt cattaactgg ttgtggaact atattaacta
     9721 cgacaggaat atcaggttga ttattccacc ctataaaagg aagtagtatc aatttatatc
     9781 tgtgacagct attttagaat aacaaaatat tcctttatca attcggtaat tgaattatgt
     9841 ttcaactact cgccaaactc aactgctgta tttagttttt gcgataacag ttttaacaag
     9901 tttgatttta gtgcatcgtt attgattttc tcaataacat cactggcaaa tgtataattg
     9961 agcaatgcac gtgcatcgcg ttctccgata ccacgcgaac ggaaataaaa caatgcatct
    10021 tcgtctaact gacctgtagt ggcaccatgt gagcatttta catcatctgc ataaatttca
    10081 agctgtggct ttgcattcat cacagcagtg ttactcagca acacattctt gctactttga
    10141 aaagcattgg tcttctgcgc atcttttcta acaaaaatct ttccattaaa aacgccctgc
    10201 gaatggtcgt ctaaaatatt tttatataac tgattgctct cacagtttgg acttgcatga
    10261 tctaccactg tgtgattatc cacaacctga tagccatcag caacagtgag tccgttaagg
    10321 tgggcacatg cattgggttc acttaaaata atctcgaggt tgttacgaac caaagcacct
    10381 cccatcgtta ctgtaaaaat atcgaacaca gctttacgct gtacacgggc ataaactctg
    10441 ttaatgtggt tggcattact attctcattc tgcttaatgt cgaattcaac aactgcacct
    10501 tcaccaatca gaaactctgt tactgtattg gtgagtgatg gaaaattatt cagtgaatga
    10561 taagttgcta ttacctgaaa tgctgaattt tttcctgcaa caacaataca tttaggatgt
    10621 gcagcaacgg cttcatgctg cgaatcgtta acataaagca gatgtattgc cattggcata
    10681 cgacaattat caggaacatg aatcataata ccaccctgat gaaaagctgt gtttaatgca
    10741 tgaaaaggtt cgtcttctga attacgtaaa gtctctaaat agagttctgc ttctttactt
    10801 tcgagcgaat aaatactgaa aggatgcttg tggctttgat gtccggaaaa ctcagaacaa
    10861 taacgtccat tgataaaagt caagtttact gtatcactgc cggcatcgta aaattgttcc
    10921 agatccgaag ctttcagtgg cgatgcaacc atacagtgat tatatttagt ttcactgata
    10981 ggtttaagat taagatattt ccactcttca tgttttgaat ccggaaatcc attctttaaa
    11041 aatttattta aagctgcgag acgcaatttt tgcagtccgt caacgggaat attctcttcg
    11101 gcaatgcttg tttcgttagt cacagaaagt gtatcaacag atgagttcac aataatcagg
    11161 tttaaatttc aacttcttct tttatccaat catatccttt ttcttccaat tcaagagcca
    11221 gttctttcgg tccgctctta acaatttttc ctttatacaa aacgtgtaca aaatcgggaa
    11281 ctatgtaatc gagcaaacgc tgatagtgtg taataactat aaaagatcgt tttccgtcac
    11341 gcaatgcatt aataccatca gacacaattc tgagtgcatc aatatcaaga cctgaatcgg
    11401 tttcatcaag tacacacaac tccggctcaa gcatggccat ctgaaaaatt tcattacgtt
    11461 ttttctctcc tccactgaat ccttcattaa cactgcgctg tgtcatgctt ttatccatct
    11521 tcaccaactg cattttatct ttcatcagct ttaagaagtc ttttgaatcg agtgcctcta
    11581 aacctttcga cttccgaact tcgttaacag cagttttcag aaagttggca ttactcactc
    11641 ccggtatttc tatcggatac tgaaacgcaa gaaatatccc tttacgggct ctgtcttcgg
    11701 gagaaagttc gataagactt tcaccatgat acaatacttc accctcatta acttcatacg
    11761 cttcgcgtcc agcaagtact gatgccaacg tactttttcc tgaaccatta ggtcccatta
    11821 tggcatgtac ttctcccggg tttacctgaa ggtttaatcc gtttaatatt tctttacctt
    11881 caactgaggc atgtagattt ttaatgttta acatactttt taaaaaaaac tttattcttc
    11941 tgaactaact ataattaaat tttatcctac tgatccttcc aaagaaacag ctaacagttt
    12001 ttgtgcttca acagcaaact ccatcggtaa ctgattaaac acttctttgc aataaccatt
    12061 tactataagt ccgattgcat cttcagtaga gatgcctcgt tgattgcaat aaaatatctg
    12121 atcctctcca acttttgatg ttgttgcctc atgctcaacc ttggcagtag gatttgcaca
    12181 ttccagatat ggaaaggtat gtgcaccaca tttatcacca aggagcaagg aatcgcattg
    12241 cgaaaaattt cgtgctttct ctgcctgttt accaatacgg actaatccac gataactatt
    12301 gttgctgcga cctccgctta ttcctttact gatgatggta ctgcgcgtat gtttgccgat
    12361 atgaatcatc tttgtaccgg tgtcggcctg ctggtagttg ttggttaatg caacagaata
    12421 aaattctcct gttgaataat cgccttttaa tattacactt ggatatttcc atgtcactgc
    12481 actacccgtt tcaacctgtg tccagctgat tttagaacgc tctcctgcac acagtccacg
    12541 cttggtaaca aaattaaaaa taccgcccct tccatctttg tcaccgggat accagttttg
    12601 tactgtacta tattttacct gtgcatcggt atgcgcataa atttctacta ctgctgcatg
    12661 caattgattc tcgtcacgct tgggtgctgt gcagccttcg agataactaa cataagcacc
    12721 ttcatcggca atgattagtg ttctttcaaa ctgacctgta ccagctgagt ttattcggaa
    12781 ataggtactc aactccattg ggcaacgaac gcctttgggt atataacaaa aagagccgtc
    12841 actgaaaacg gcactgttta atgcagcata aaaattgtca gtatagggta ctacactacc
    12901 catatatttt tttaccagct ccggatgttc ctgaacagct tcactgaatg atgaaaaaat
    12961 aattccaagt ttacttaatt cctgacggaa ggtagttttt acacttacac tgtctattac
    13021 tgcatcaaca gctacaccgg caaggcgttt ttgctctagt aatgatattc caagtttatc
    13081 aaatgtagct ttgatttccg ggtcaagttc atctaaactg ttgagttgtt tcttgggttt
    13141 gggcgctgag tagtaaatga tatcctgaaa atcaactttc ggatagttga cattagccca
    13201 ttgcggctcc tgcatttttt gccagtgacg aaatgctttt aatcgaaatt caagcatcca
    13261 ctccggttct tttttcttgg ctgaaataaa tcttacaata tcttcagaca atcctttggg
    13321 agcattctct gattcaatgt tacttacaaa tccccactta tactcgccac ttgttacctc
    13381 atctattatt tttaaatctt cgctcactgt tttacaaaat ttgtttattt agattatttc
    13441 taaaattaat tattggataa aaaaaattta cctacatagt ttatactgca aaactttctc
    13501 cgcaaccaca ggttcttgat gcatttgggt tattaaactg aaaccctttg ccatttaaac
    13561 catctgtgta atcgagttct gtacctacta aatacaaaaa cgattttctg tcgcacacta
    13621 tttttattcc tttatcttca aacactttat cttccggttt cagttctgta tcaaaaacca
    13681 acatataggt taaaccagaa catccgcccc cttctactcc aacacgaata aatgcatctg
    13741 caggcttgtt ttccgcctgc attaaactta aggcttttga tttggctttt tcagttactg
    13801 ttatcatttt caagtactta taaaagtggt gcaaagatag gtttaattgc tgttatagtc
    13861 aacaattaca tgtgataaaa gtttatcaaa gttgattcat aaccagattt ttacttttgt
    13921 cgcattcaat aaagaattat gaatcatgtt tgaaaacgca aacaaccgca cagagttaag
    13981 tagtttaggt gagttcgggc ttatcaggca cctgcaaaaa gccgttgaaa tcaacaataa
    14041 agaaacactg aaaggtattg gtgatgatgc tgctgtaatt agcttagata gtgatgaaaa
    14101 tgttcagctt attactactg atatgctgct tgaaggtgta cactttgacc ttgtttactg
    14161 tccgctaaaa catttgggtt ataaatcggt agtagtaaac ctttctgata tttacgccat
    14221 gaatggcatg cccaaacaaa ttgttgtggg aataggctta agtaaccgct tttcactcga
    14281 agccattgag gaactttata gtggaatgtt gcttgcatgc aaacattatg gtattgattt
    14341 gattggtggt gatactacca gtagccgcag tggtttagtg atttcaatta ctgccattgg
    14401 tacagcaaaa aaatctgatg ttgtttacag aaacggtgct caggaaaatg atttgatttg
    14461 tgttagtggt gatcttggtg ctgcatacct gggtctgcaa ctgctcaacc gcgaaaagaa
    14521 agttttttta gacagccccg gtgtgcaacc cgattttcaa ggtaatgatt atttgcttga
    14581 gcgacagtta aagcccgaag caaggaaaga aatagttgaa atattgaaaa aaacaggagt
    14641 aaaacctact gccatgattg acatcagcga tgggttggct tctgaaatta tgcacatctg
    14701 tacacagtca gacaaaggct gcacaatcta tgaagctaaa gttcctattg atgaaaaaac
    14761 ttataacctt gcacgtgagt ttcatttaga tccaacagta tgtgccatga acggtggtga
    14821 agattacgaa ctgttgttta ctatcaacca aaaagatttt gaaaaaatta acagtgcagc
    14881 tgatatatcc attatcggtc acatcactgc caaggaggat ggctattata tgcttgataa
    14941 atcagaccga aaagtagcat tgaaagcaca aggctgggat gcattaaaat cataaaaaat
    15001 attactatga gtcaaaaaaa ctaccgcata ctttgctgga atgtcaatgg cattagggcc
    15061 atttggaaaa aaggatttcc tgaatggtta acaaaagaaa tgcccgatgt attatgtctt
    15121 caggaaacca aggcacaacc ggaacaattg gatgatgaaa taaaaaattt cagtggctat
    15181 aaaagttatt ttttttctgc acagaaaaaa ggatacagtg gcgttgctat ctacaccaaa
    15241 catgagccat tgaaagtaag atatggatta gatgatgata cctttgatgt agaaggacgt
    15301 tgtattgaaa tggagtttga agattttgta ttatacactg catactttcc taatggtggt
    15361 cgtggtcccg aacgggttaa gtataaattg gatttctatg attcgctttt tcagagagca
    15421 gaaaacatga gaaagaaaaa taaaaacatt gtcatttgcg gagactataa tactgcacac
    15481 aaggaaatag atttggcacg accgaaagaa aattcaaaga caacagggtt tttgccaata
    15541 gaacgcgaat ggattgataa aataattgat atgaagtatg tagatatttt cagagagttc
    15601 aataaagaag cagggcaata cacctactgg gatcagatta cacgtgcccg cgaccgaaat
    15661 gtaggctggc gcatagatta ctatatgatt tctgaagaac ttcgtaaata cgtgaaaagc
    15721 gcaccaatac acatgaatgt tatgggcagc gatcattgtc caattgaatt gaatttgtct
    15781 ttctgacaat actttcaaaa aactatcaca ctttataaaa acaatttgtt gacaaacatt
    15841 attgcacggc ttatcaggtt acgtgcatga ggtggataag gctgatagcc attctgaaga
    15901 taagagttta ccaaagaata ttttctgtca gccataaaaa cgggtttcac ctttacgaag
    15961 tagtaaagca gataagtcat cattgctcca taagcagagc ctatgataaa tccgattact
    16021 acatttctgt ctatttttat agccagtatc aacccccaaa gcgcaaagaa taactttagt
    16081 actctgccaa tggtatgata tacctgtgca taatgacttc tgggaatata aatgttgtgt
    16141 ttggtgttga ctaaatattt tctgtttgtt cctcctttct cggcattata ccataatgaa
    16201 acctgtaaag gttttccgtc tgcaaactta tcaggagaaa aaaaacattt gccaggcgca
    16261 acctgaataa attgagatgt ggtgaaaaag gttttaccaa ataatttaat ggcagcacga
    16321 ttaattgtaa aattacctgt tcgctttgct gccagtacat atcgaattgt tgtttcccgt
    16381 gcattttttc ccgaccgata tacgtcttga aatgacacaa atggtttgcc ggctatataa
    16441 aaatcacgca aatcaggctt ttgcataaat cgcccttcac cggtataggt aaatgtcaac
    16501 tcaaactctt cgcacacttt tacattatta ctgctgacat acatttcgaa ataatggtta
    16561 aactgatcgg cattgctgcg cgaataactt tgatattgct tatcaaaatt tcttcttttc
    16621 tcggtgtctg tcagtaaggc ataagcctca ttcaattctc tgaaaagttc ctcagcttga
    16681 tcgttgctgt tgcggtcagg gtgcagttcg aaggcttttt tacgataagc ccgcttgatc
    16741 tcctccggtg tagccgaata agatacgccc aaaatgatat atggatcttt ctgcatttag
    16801 tgcctgtggg taacgattaa acaatattga acatttttgt gttagtttgc aactgttttt
    16861 cagcatttat ttataacaat actttatttg aaaaattatt ttaaaatact tcgattcgta
    16921 aaaccttatt ggggctatgc attattgaat gcatcatgca acattttatc ggttctgttt
    16981 tcattttttt cactaacgtt aattgctccc tttcttgatt tattgtttct gaaagatgat
    17041 aacttttatt tagagaaaat ggctgcgggg aaaccggagt ttcatttgtc aacaaaggct
    17101 attgttgata gtttctatta ttatcttact gagatgataa ctgaccccaa cagaggtaag
    17161 caatatgcgt taatgtttat ttgtattgca gtggctgtga tgttttttct taaaaacctg
    17221 tttcgctatt taggtcagtt ttttatatcc cctatcagaa acggtgttgt aaaagatatt
    17281 cgtaccgaag tacataaaaa gataatgcgg ttgcctttat catggtttgg caatgaaaga
    17341 aaaggtgata tcatcaccag agttacctct gatgtgcatg aagtagaatg gagtgtaatg
    17401 agttctttgg aagtggtttt ccgtgacccg ttcaacatat tcctgttttt gggaatgatg
    17461 atttttatta gtgccaaact cacagttttc gttttcattc tacttccaat agttggtttt
    17521 ataattggac gaataggtaa aagtctaaaa cgtacatctg caaagagcaa agaaaaactt
    17581 ggtctattaa tttcaattat tgaagaaact ctttccggtc tgcgtattat taaagccttc
    17641 aatgcagaaa attttatgtc agaaaaattt tcgaaagtaa atgagcaata caataccatt
    17701 gccatccgtg cattacgtaa aacagattta tcatcaccgc tgagcgagtt tttaggtacg
    17761 gtaactttaa tgattgtaat gtattttgga ggtatgcttg tgttggaaaa agatgcatta
    17821 ttatctgcct cagcatttat aacttacatt gcactgtttt cgcagattat accacctgca
    17881 aaatctttca ctcaggcatt ttacaatatt caaaaagggc ttgcttctgc cgacagaata
    17941 tttgctgttt tagaagcaga agaaagtatt aaagatcatc agtcggcaga aacaattaag
    18001 tcattcaatg ataaaattga ataccaaaat atttcttttg cttacagaaa aggtgatgag
    18061 ggttatgtct taaaaaatgt aagttttact attgataaag gaaaaactat tgcattggtg
    18121 ggccaatcag gatcaggcaa aacaacattg gcagatttat tgcctcgcta ttatgacgtt
    18181 gatgcaggtg cattacttat tgacggcaca gatatcagaa aattgcaatt gcataccatc
    18241 agaagtttgc tgggtgttgt atcgcaggaa cctattcttt ttaacgatac tatttataat
    18301 aacattgttt ttggcatgag caatgcaaca gaaaaaatga ttgaagaagc tgcaagagtt
    18361 gccaatgcac atgagtttat tatgcaatta ccggaagggt atcagacaaa tattggtgac
    18421 agaggcagta aactctccgg tggacagcgg caaagaatta gtatagcacg cgctgtacta
    18481 aaaaatccac ccattttgat tctagacgaa gctacatctg cattagatac agaaagcgaa
    18541 aagttggttc aggatgcgct gacaagcctg atgaaaaacc gaacagtatt ggtgattgca
    18601 cacagactga gcaccataca acatgccgat gaaattattg ttttgcaaaa aggagaaatt
    18661 gtacagcgag gcacacatgc ccaacttgca aatgttgaag gagtatataa aaagctttac
    18721 gaactacaag gcttaagttc ctgaacgagc ttttacttcc ctgccaacca ctgtttaaaa
    18781 actccactgc gctccgtact aactatcgta tcctgagtgg ttgatggatg aagtgtgagc
    18841 ttaactcttg atttagaaac gacattcatt ttctgaatag ctttaaagtt tacgataaac
    18901 tgtctgttaa ttctgaagaa tacagccgga tcaagaatca actcaagctc gtctaagttg
    18961 taatcaataa taaatgattc tccattaaaa gtacaaagat tacttatctt attctctgtg
    19021 aaaaaataag cagcttcttc aatttctact gtctttatag tatctgcaaa acgaattaca
    19081 atacgcttct gaaaaccttt tttttcttgt aacagcgtct gtaacaaaac atcataatta
    19141 actccggaag ttgagtctga aaaccaattc tcatatttct gtattgcagt ttttagctct
    19201 tctcttttta caggctttaa taaataatca attccgttgt ttcgaaatgc ttctattgca
    19261 tattcatcat aagccgtact gaatataaca ggaagcttta cagtgtgact tttaaaaatt
    19321 tcaaaactca atccatctgc aagcctgatg tctgaaataa taagatcgaa acttttattc
    19381 tgcttcataa attcctttcc ggaacgtacc gagtcaatta ctgaaacaat ctcaacatct
    19441 gtactaacct cattaaggag tttaatcagt ctgcgtgcgg ccggctcttc gtcttcaaaa
    19501 attactatct tcattactcg cgaataatca atggcaatga tacaacaaaa tattcttgtg
    19561 atgattcaat ctgtacagtt ttatctgtga gcaaacgata acgtgctata atattacgaa
    19621 gtccggttcc tgttgattca tctttattca ttttggggtt ggtattatta ctgatgatta
    19681 ttcttgaatc ggaaaacgaa acctttactt tcaggttttt ttctttggaa atctcattgt
    19741 gtttaattgc attctcgagt aataattgca acaccattgg aacaacatac ctgtcatcat
    19801 aaaggctgtg ttcgtcaact aaaagttgca aatttgaacc aaatcttttt tgttgaataa
    19861 aataaaatgc ttttacgatt ttaatttctt cagatagttt tatggtatct ttatccttgt
    19921 aggtaacaat gtttctgaaa aaggcagaaa ggttttctac atactcaact gcatgctctc
    19981 tgtcatgttc aatgatatct atgagtgtgt taaaactatt aaacagaaaa tgcgggttca
    20041 cttgattgcg taatgtctca aactgaaacc tgatggaatc gttttttagt tgctgcaact
    20101 ttttcattct tgcatcccta agtctgataa aaaagtaaac taataaaata gtaaacagag
    20161 cacttgtaat gtaaaaccat tcctcactcc aaaatggctt ttttatttca aacgaaaaaa
    20221 ttctctcatt gttgaatgta aactgtggat ctattgaagt cctgactctg aaaacatatt
    20281 tacctgatgg cagattagga aatacttgtt ttacgtcctt cgtttcaatc cagtcagagt
    20341 ttaaaccgtc taacttatac tgataaagaa ttcttccggg attggtgaac cacgttgcag
    20401 catattcaaa tgaaattgtg ttctcattgt gtctgaacac aggcatcatt cctgaataat
    20461 ttctgaaaat cggttgtatc atgtttaatt tactcaactg cggaacagta ttttgtttca
    20521 gcaactttgg atctaatact accatacctt tggctgagcc aaaccaaatt cttccgagac
    20581 tatctttagc tacagaatta atgtcagggt tcaggttatc gagattattt tctgttccaa
    20641 gatacataac tgaacctgtc ttataattca gtatatcaat gcctctttta ttgaccattg
    20701 caatactgct atcgtcaagc acagcaatac ttgttatatt caaatcgcgg attccattgg
    20761 agcggttata taatttataa ctgtcaccat taagacaaac aattccttca tcagcaacac
    20821 taaaccatat tgcatcattc ttatcacccg ttattgaata tactgtttta cctttaaact
    20881 tccccttaaa aggagtaaca aacttatctc tatctaacaa cactattccg ttctgatcgg
    20941 taccgaacca aaaacgtcct tttacatcac gataaacaca ataaacatag taatttccca
    21001 acagtgattc atgagaaaaa cttctgattt tactctgaag attgtcagta tttaaatttt
    21061 tcgtttcaat taagacagca cctccaaatg ttccaagcca aacatttttt tcattaccat
    21121 taatatataa aatactgcta taatcaaatg ggacttctat tttcttaatt tcattctttg
    21181 aaggatttat cctgaaaaca ccactaccca aggtgcccac ccatatatac ttttctttat
    21241 caatatataa accggaaaca tcaatatttt tttctgatgg gagaattgtc cattctttta
    21301 ctgttgatgt attaaatgat atgagcataa ctttgttttt aatactcatt accatttgat
    21361 tgctatcaag aaaagtgact tcatgaacat ttttaatatc aactccgcta tattgatcta
    21421 aaaatgtcag ccattgtcca tgtgaacgat ataaaccacg gtcagttgca agccacacat
    21481 tattttgatg atctattgct actgaataga cagtgctaaa aaatttatct gactgttgtt
    21541 tgaaagaaaa aatattttct tcaggacgtc tgcaatataa tagtcccccg gattccgttg
    21601 ccatccaaat ttcatgtgtt agtctgttta gaataatgtc atttactgca ccatatttcc
    21661 aaactgaatc ttcttgttgt tttagaaatt tattctgttt aatatcaaag atgcataatc
    21721 cgttttcctg tgtacctaat agtaattttg aattttcaat aattattttt tttactatat
    21781 tatcacacaa tccatcagca gcctgaagtt tacggagaac tttcttttct ttatttgatg
    21841 tacacaacga cagtccctga tccgttccaa tccaaatatt accggattta tcaagggctg
    21901 aagtgtagca gaagtcatca cttaacccat cttctgttcc tatatgataa aactttttat
    21961 ccttaaaata atacactcca ttacctgctg tcgtaaacca tttaatacca ttgccatcat
    22021 cattgattga tgtaatggca actgctgtct ttagtgcaaa tactttgatt atttttaata
    22081 tatcatcttc aacgaagcca attttaccat ccttggtacc tacccaaatt actcccgtag
    22141 catcttcaaa taatgccgtt acgttacttt ttatcgtatc attgactgca agcaaatgat
    22201 gattgtttcc atcactacgg taaaggccat ctgaagtacc agcccaaaga aatccgttct
    22261 tacaaaacaa taaagcattg acagttaagt ttggtgaatc tctgtttaac gaaatttgtt
    22321 tataaaaaga attctgcccg tttacagttt gtacaattaa gaacagaaaa acaataattc
    22381 ttttcattta cgtcttaatg tggcttttac ctgtaccaaa acttcgttgg ctattttatc
    22441 ctgaacaaca cgaggtactt ttatcttatg atcttttaag ggaacactaa aaacagataa
    22501 aatagttatg ccatttccct gaacagaaat ttttgactta ataattcttt cctgttcaac
    22561 accatgaatt attagtttgc ctttggctct aatggttttt tcattgggtt tagaaatatc
    22621 aacatcttca ataatcttgc ctgtgaaagt tgctacagga aatttatcac tttccatata
    22681 acgatcaaga aagtgctctc tttgtaattc ggcattgaat cctttaaaag aattgatggg
    22741 aattaaaaac tcaaatattt tcttttgaaa atcaatcatg gcttccaact tttgagattt
    22801 agcatcaatt aattcaagtt tggcctcgct gcgaaaaaac acttcgccat tagtactgta
    22861 atataccaac tgcccattaa ccttgacaga aaagatcaaa ataaaaaaca atattgttaa
    22921 aacaccacct tgtttcatta aaacaaaaat attaatctct atggatatta ccatagttta
    22981 ctttaatgcc ctaaatttgt gtttttgatg attgattatg tattctgaac aaagtcataa
    23041 attccacggt cagctattta agtatgcaac ggagagcgtt gttgtaacca attcaaaagg
    23101 aatcattgta acagtaaatc cttcggcatt aaaactcttt ggatacactg agaatgaaat
    23161 tataggtcag aaaattgaaa agttaatggc ttcacgtttt gaagcatcac atcaattgca
    23221 cagagaaggt tttaataaaa atccacataa tcgtgctatg ggaattggcc tcgatttgca
    23281 tgctatgcgt aaagatggca gtgaatttcc ggtagaaata agtctgagtc ctttttcttt
    23341 aaatgacgaa tcgtatgtaa tggcatttat tattgacata ac
//
LOCUS       DAOQNP010000047        23377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_749_length_23377_cov_8.321242, whole genome shotgun sequence.
ACCESSION   DAOQNP010000047 DAOQNP010000000
VERSION     DAOQNP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_23377_cov_8.321242"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="PKY09_07710"
     CDS             <1..824
                     /locus_tag="PKY09_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNB12783.1"
                     /translation="TINGKYPARQDAINFLLKTIKQIEVRSPVGKAALQNVLKDLSST
                     GTKIEIYSKGDKIKTYYVGGPTQDMLGTFMYLENSTVPFVMHIPGFNGYLTTRYFASA
                     DEWRSRIIFQYGEGQIASIMVQNNQHPDESFRVKRDEDHYVYYSSLTATTPSEIFQSQ
                     LVAYLAKYQLIGFERPTYDLDVDYRDSLLKTQPIRILEVEGVDGKKDKIEMFLKPVDT
                     GTMTSVDERTGLLREFDPDRMYASWNNDTNLIVVQYHVFDKLFSKPDRIKGFGGL"
     gene            892..2013
                     /gene="dnaN"
                     /locus_tag="PKY09_07715"
     CDS             892..2013
                     /gene="dnaN"
                     /locus_tag="PKY09_07715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNB12784.1"
                     /translation="MKLIVSSLVLQKHLQSISGVLTTNSPLPILDNFLFAVNGSELTL
                     SASDLETTITTRLKVESKDKGIIAIPGKILLDILKTFNEQPLTFTINEKNHTVEISSD
                     YGKYKLNGQNGEDFPRIPVLEKSNHIELPSSVLHEAINKTIFATGNDDLRPVMSGVFC
                     QLSQDSTIFVATDAHRLVRYRRTDIKSKEAGSFILPKKPLNLLKNILPQEETRVKVDY
                     NEANAFFSFDNYDLICRLIDGRYPNYEAVIPVDNPNVLTVDRVAFLNSIKRVSIFSNK
                     TTYQVKLAIKGSELAISAEDVDYANEANERLTCSYDGADMDIAFNARFLADIVSNLDT
                     EEIHLHMSLSNRAGLLTPGKDHTNAAEDILMLIMPVMLG"
     gene            2078..2797
                     /locus_tag="PKY09_07720"
     CDS             2078..2797
                     /locus_tag="PKY09_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNB12785.1"
                     /translation="METRQQKYAERYKDVIGAAFNFESSQLELPLYYFVSGFAHPFLP
                     IIKHDGLFLFEWGLIPSWIKDAKNASEIRAKTLNAVGETIFEKPSFRKSILTNRCLLG
                     ISGFYEWRDVNKKKYPYFIKTKHSEIFSLGCIYETWVDKNTGEMHNTFSIITTPANSM
                     MEKIHNIKKRMPLIISKEDERKWVDPVSDISEIKSLIKPYDASEMTANTISQAANSAR
                     ANRNVPEIVNHVSYPELTFLD"
     gene            complement(2812..3561)
                     /locus_tag="PKY09_07725"
     CDS             complement(2812..3561)
                     /locus_tag="PKY09_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB12786.1"
                     /translation="MKEIKAIVVDDEKFGREQIKSLLKRYFQQVDLVAEAADAVEALR
                     QIYFHKPDLVFLDIEMPDKGGFEMLENIQEHDFEIIFVTAYDQYALKAIKFSALDYLL
                     KPVNKEEFKSAINKFLDKRKNAYDRKSVINNFIRNYTSQSTDSFRLAVNSALKTFFLL
                     PEEIIRCEADGNYTKITCSNSDTILSSKTLKEFDDLLSDFDFIRVHRAHLVNKKFIRS
                     FNNENMLLLQDNTAIEVSRRKRGEVRNLFGQ"
     gene            complement(3601..6489)
                     /locus_tag="PKY09_07730"
     CDS             complement(3601..6489)
                     /locus_tag="PKY09_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12787.1"
                     /translation="MKRFVTYLFLFLLLCLTGRSQDASKIPLYLNDLKQAANDSARVE
                     AYRDLCFNYSTTNTDSGIYFGKKGLALALSIKFIKGIGDCYNSLGWCYTRKGEFVEAK
                     KCLLLALNYFNQTGNRCHTAVALSNLGGMYFNQNQLADALEYYLKCIQLSKGCPESWL
                     LPGSLYSIGIVYNSQKEFKIAIPYFGQAMEINILNNDSNKLADCINGLGNAYLGLKQL
                     DSAAFYLNQSSAMYHTLGNVSGEAYALESIGSLHLEHGRINEALKFFEMARTNFEKSG
                     SKADVCYELIMIGNVYQKQGKINSAITYMQMALQIAERNKFSSLESQVLNSLSDLYAS
                     KGDYKNAFDFFKRESAIKDSTRESQQQIRLDDLKTKYETEQKDKMIELLNRDKSIEKA
                     NAEWQRLLKNIFIAGAVMLFLILLVIYNRFRLKRETAKKLEQKNKIIAYEKDRAESSE
                     RFKQQFLANMSHEIRTPLNAIIGMTELLQETKQDELNEKYIQIIKNSSDGLLVIINDI
                     LDLSKIEAGKMELEKIPFRFQDILDHVYEVFSKKASTKGIDFKFDVSEDIPEFIIADP
                     FRITQVLINIVGNAIKFTDNGFVIVSVRKTQSLQSQNKDLCRLQFEVSDSGIGIPKNQ
                     QERIFDSFIQTNAYNTRKYGGTGLGLTISQSLVRLMGGNIDVSSVENQGTTFSFNIDV
                     EKSALIPEAKKTEHIPISADSVKKDFSILLVEDNEYNQILVKDALNKKIPEVKLHIVR
                     SGLEAIAALQTCRFNLILMDISMLEMDGYELTAIIRNDLNEATSKIPIVALTANVSQH
                     EYDKCLSAGMNDYLTKPFKIQELVDVVNRFRFNPKDEAGSEVTEKTYKSADIDLSYLI
                     DFTDGDITEMSRYVDIYLQRIPEVFREVEMALKNNEVKQIIVLLHSIKPMIASVGLRN
                     CEQKISEIESQRESIKKNKLMPVILQIRKQCLEMVDKLNQWKQKNKID"
     gene            6665..8293
                     /locus_tag="PKY09_07735"
     CDS             6665..8293
                     /locus_tag="PKY09_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12788.1"
                     /translation="MKNLYILLWILLTAYNTKAQFSNSPAQPLSVCNSTATKENLQIL
                     PDGSGGYFTFWIDNRTGTIKKLYGQHLDAAGNEQWTANGKTIIQHPTQSVNAFHCTLF
                     NNGILFTWVQSGASYQDSLLVKKVNFSGNDVWAQPTLIDKSSQAASILGIETYSMNVI
                     ANDSGAFVNYSVVYYGGSSGPKFNRIDFNGVKQWSTSKTTSLPGYTYLYYKGPQNTFF
                     SISRGNGLGSGMYIQKYNLQSIPQWANAVDISQGVNTNGFGGLQYLFSDNAGNSYIVW
                     ESMSNKTLLTKLDAAGSFVWTPQFKATVGLANTGQNRPHATFYNNKLYIVWMDNRVAN
                     QTTVYAQAYNSSGATQWLTDGVELGENNYYYSYPKIAVSDSNAVLVAYNNPSLQSIVG
                     HRIKSNGTLAWPLSGIDLATDHSWISYNSITAINDTNGCNAIFWNESSTSHIWGAKIC
                     SNGVLVNTIDNSKPDFEISPNPASDNLNIKLGSILNSTAISIIDMNGKIIMEMNIPEP
                     INELNLDLKGLAKGIYALSITSKDIRQTKKLIIK"
     gene            8354..8488
                     /locus_tag="PKY09_07740"
     CDS             8354..8488
                     /locus_tag="PKY09_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12789.1"
                     /translation="MSKQPPKIDHEIIREKTLDRILFILEIVLVITLIALCGAIITSL
                     "
     gene            9024..9500
                     /locus_tag="PKY09_07745"
     CDS             9024..9500
                     /locus_tag="PKY09_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HNB12790.1"
                     /translation="MSSLNADKDSTGVKNLLKKRFWFTLILIVVLGIFAAATLPKSPY
                     FTFANETPSTVIHVGTMQFSFFLSTKPIDPKSPSGETIEIPHDQLVEFRVTSFDVNHG
                     FAVYDPSMRLITQVQAMPGYVNNLRYKFTEPGKYHIFCLEFCGTGHPVMQATFKVI"
     gene            9515..10966
                     /locus_tag="PKY09_07750"
     CDS             9515..10966
                     /locus_tag="PKY09_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNB12791.1"
                     /translation="MNIESLKLKKISALWIIVILVLFIASIVLGILMRLNQGKVIEQS
                     PLTFYTNMTTHGLTMIGIWFAAGMVAVNYLLQRYVKTSYGVNLFAFILTVIGVVMLWI
                     TTYAGKFSTGWTFLYPLPFRDLGSESWSTPLFLISLTVLGVGWLVWSVSLMAQILKKY
                     TLSQAFAWQHFKSNASVETPPFILITTVTLIGVIASLIAAVIVIILYFAEYFSHGDFV
                     NDALLMKNLIYFFGHTIANEMLYLGLAVIYEIFGEVSDRPKWKMTWYISLAWNCAIIF
                     ILTAFFHHLYMDFVQPQALQIVGQLASYLASIPSAAVTVFSIVAAVYGVKVRWSIVNL
                     LFFLGVAGWVIGGLGAVIDATISNNIMLHNTLWVPAHFHTYNAMGNVLFSLGFFYWFS
                     TQYVDKLVLSKLTKYTLALLIIGGIGFLMAFYVAGANSIPRRYSTYPVELTSGPYLAK
                     VGALFASIYLIAILIFFYNIAKRCFVILSASSR"
     gene            10933..11685
                     /locus_tag="PKY09_07755"
     CDS             10933..11685
                     /locus_tag="PKY09_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB12792.1"
                     /translation="MFRYFICFFTLACFSFFVYANDNHSELREEQNIYGKIYNAPLHL
                     SGSSKVTSVESLYSRKPLIVALVFTRCYGICNPYLLQLSENIKQLQSNTDFNVLVVSF
                     DPRDNLKDMTNLAKQYRLENNRQWNFATTDSINEFINSIGFNPKWDSITQQFDHDALL
                     VGVNEKGLITKNLLGIRTVDDLQLMIRSIHNVFIPSYRLPTKSNIFSCFNYDPKTQTN
                     RPGLGLLFVALPAIITFLIVIIIVIKSKRAAA"
     gene            11816..12169
                     /locus_tag="PKY09_07760"
     CDS             11816..12169
                     /locus_tag="PKY09_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNB12793.1"
                     /translation="MSSIKIKRIYEAPDKSDGYRILVDRLWPRGIKKEYADIDEWSKD
                     VAPSSELREWFGHDPEKFKEFTARYSKELHSKKEVLKHIVEIAKKQNLTLLYGAKDTE
                     HNQAVVLQKVLNKML"
     gene            12256..12633
                     /locus_tag="PKY09_07765"
     CDS             12256..12633
                     /locus_tag="PKY09_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB12794.1"
                     /translation="MGLKKQIEPLRMAKYAKWFYAEHLIPHFEIEEKYLFPVLTPENE
                     MIQKAIKEHRMLTDLCKAEAELATNAKLVMLEKTLEAHIRFEERVLFKEIESIATSDQ
                     LELINKQHKEEKFVDNLADQFWK"
     gene            12661..13092
                     /locus_tag="PKY09_07770"
     CDS             12661..13092
                     /locus_tag="PKY09_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNB12795.1"
                     /translation="MLHSNIKTENILIARNTRPTCMRILVYNFLAKQHAALSITEIEK
                     HFQHADRITIYRTLKTFEEKGFIHSIQEDNITKYTLCGSACSEQSHYDQHLHFYCKAC
                     KQTTCKTDIAIPQNNIKGFKIDDITFFARGICENCNNSTMQ"
     gene            13144..15114
                     /locus_tag="PKY09_07775"
     CDS             13144..15114
                     /locus_tag="PKY09_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNB12796.1"
                     /translation="MQKHIYNSVGEQSCCSIEDNNNKHVHSNELKDSHHEAGLFSMYA
                     PGLFSLVLLLAGMHFDYWNTFFWFNGWIRLAWYIIAYIPVGFPVIKNAYLGITKGNIF
                     SEFFLMAIATLGAFAIKEYPEGVAVMLFYTVGELLQTLAVKRAKSNIKTLLDQRPEVA
                     TVLKNNNAVSMSASDVAIGEIILLKPGEKLALDGILLSDKGAFNTAALTGESMPDSKL
                     KGELVLAGMINLNTVVQIKVTAAYEDSKISRMLELIQNASAQKAQTELFIRKFAKVYT
                     PIVVGLAVAITLLPYFFTHEYIFREWFYRALIFLVISCPCALVISIPLGYFGGIGAAG
                     RNGILVKGSNFLDVLASVQHVILDKTGTITEGVFEVQDVKIIPAEDRDKILQMVNAIE
                     FHSTHPVATAIHNYVGNTTTIELNQVEEISGHGMKGFVNGKELLVGNFKLLSLHNVSF
                     DIDPVSIKNTVIAIAFDGKFSGYITIADNIKPFAAVAIQKLKQMNVDVTMLSGDKLTV
                     VDSVADQTAISHAYAELLPDDKVEKIKSIINKMQTVAFVGDGVNDAPAIAISHVGIAM
                     GGLGSDAAIEVADVVIQDDNPIKIPLAINIGKQTKKVVWQNITLAFAIKAMVLLLGAG
                     GIATMWEAVFADVGVALLAILNATRLLHFKFK"
     gene            complement(15289..16251)
                     /locus_tag="PKY09_07780"
     CDS             complement(15289..16251)
                     /locus_tag="PKY09_07780"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNB12797.1"
                     /translation="MSNYLDFEKPIEELYEQLEKTKQIGTKGKIDVNNLLSEIEEKIQ
                     VAKKQIYSNLTPWQRVQLSRHPDRPYTLDYINAITDNTFIELHGDRNVKDDKAMVGGF
                     GTVDGKTIMFIGQQKGNNTKSRQYRNFGMPNPEGYRKALRLMKLAEKFNKPIVTFIDT
                     PGAYPGLEAEERGQGEAIARNLYEMSILKVPVICIIIGEGASGGALGIGIGDKVLMLE
                     NTWYSVISPESCSSILWRNWDNKEKAANALKLTATDMLSLKLIDGIIKEPAGGAHSNP
                     EEMFATVKKEINIQLGALLAVSAADRVNNRIDKFCAMGVVNKDA"
     gene            16343..17167
                     /gene="ispE"
                     /locus_tag="PKY09_07785"
     CDS             16343..17167
                     /gene="ispE"
                     /locus_tag="PKY09_07785"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNB12798.1"
                     /translation="MLNFPNAKINLGLFITSRLQDGYHTLETLFYPTNWCDILEITEN
                     KNYSKSQPEDSFHISGLSVDGPTNQNLCCKAYHLLKDKFNLPPIHIFLHKQIPTGAGL
                     GGGSSDGAHTLLLLNNLFGLNISHEILSEYALKLGSDCPFFISNKPSFAFSKGEILKP
                     APAVLKGSFLVVIKPSISISTAMAYSQVKPEIPETSIAEILEMPKEQWRYFLKNDFEK
                     TILPQFPQIEKLKTNLYEAGAYYASMSGSGSAVFGLFYDEVKLSFLKDEQHYSTWL"
     gene            complement(17168..17905)
                     /locus_tag="PKY09_07790"
     CDS             complement(17168..17905)
                     /locus_tag="PKY09_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12799.1"
                     /translation="MQLRLTALFFLLSCSVLAQDVRYDYVEDMPLRYTNEFSIGATLH
                     SSGWGFMFRKGKNLTVSRKRMFEVEAVSMKHPKEYKSVSYLSERPKTFVFGKMNSLLV
                     LHGGFCYERVLFEKAERRHFEIRWNSTGGPSLGFTKPVYLIFATGNGQRVVEKYDPNN
                     QNHDRQSIYGKAEFTKGLDELKLYPGLFLKSGVMFEYNIMNDGITAVEIGVMLDAYTK
                     KIPIMAFAKNKQVFFNLYGTFMLGRKK"
     gene            18010..18303
                     /locus_tag="PKY09_07795"
     CDS             18010..18303
                     /locus_tag="PKY09_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNB12800.1"
                     /translation="MFRKTNEESLKEVIEQLLDTYKLRDKLNQVKLMQSWDNIMGEMI
                     SKRTEKLILKDHTLYIYLNSAPLKEELNYGRERIVQLLNTALESDFIKEVVIR"
     gene            complement(18511..18583)
                     /locus_tag="PKY09_07800"
     tRNA            complement(18511..18583)
                     /locus_tag="PKY09_07800"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18548..18550),aa:Lys,seq:ttt)
     gene            complement(18673..19836)
                     /locus_tag="PKY09_07805"
     CDS             complement(18673..19836)
                     /locus_tag="PKY09_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12801.1"
                     /translation="MQVIEVNTAGHKKDFLLLPKQIYSSDANWIQPLDNDIESVFDAA
                     KNKFFKHGECTRWILKNSRDKVVGRIAAFIDKKTATKNNKQPTGGMGFFECIDDKEVA
                     FKLFDTAKEWLASRGMEAMDGPINFGERDSWWGLLTDGFHEPPYKMNYNPVYYQDFFE
                     SYGFEVYFKQYCYGLKVKNKLDEKFYERHALINADKDFKAIHLKKSQLEKFAEDFRII
                     YNKAWVKHGSGKELESKQVQQFFKKMKPVIDEKIIWYVYYKNEPIAFWVNLPDINQIF
                     KYFNGKFGWFEKLRFILMLKRRVSKRMIGLVFGVIPEFQGKGVDSFMIIEGATLIRNA
                     TKYEDCELQWIGDFNPKMVSVAESLGSWRSRTLFTYRYLFDRNKPFERHPTFT"
     gene            19888..20694
                     /locus_tag="PKY09_07810"
     CDS             19888..20694
                     /locus_tag="PKY09_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB12802.1"
                     /translation="MKNKVVIITGATSGIGYACARYFAQQGCHIVIAARNEEKLKRIG
                     SELKSLYGNCLAVPTDVAIETECKNLIQKTIETFGCIDILINNAGISMRALLNDVSIN
                     VMHQVMDINFWGTVNCTKYALPYLIKSQGSVVGVSSIAGKKGLPARCAYSASKFAMEG
                     FLESVRIENLKNNLHVLVAAPGYTASNIRSTALNKDGQQQEESPLDESKLMKPETVAY
                     EIYNAVLHRKRDLVLTTNGKLTVLLNKFFPAWMDKIVYNFVAKEPDSPFK"
     gene            20705..21235
                     /locus_tag="PKY09_07815"
     CDS             20705..21235
                     /locus_tag="PKY09_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12803.1"
                     /translation="MSIKTIIFCTLSCILLAACTNTKQQLKDEIDSKEKLLYENKNGR
                     INTKLAREVIIHYRYYAEKFPDDSLSVEYLFKAGDVSVGIAAYDNALEYFKIISEKYP
                     KSNRAAYSLFLQGYISENNLEDTLSARKYYNEFLEKYPNNEMAESARFSILNLGKSAE
                     DVMKKIDGNKDSIPEK"
     gene            21238..23181
                     /locus_tag="PKY09_07820"
     CDS             21238..23181
                     /locus_tag="PKY09_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12804.1"
                     /translation="MAKSKKQKESIAEAHQKKQQQKAAEAKNHNIKILRRILALICGL
                     CGVVLYLNTLHHGFVLDDFSVILENRITKQGLKAIPQIFSSSYRSGYYLGTDELYRPI
                     PKSIFAISWNWFGENPLPLHLLNIVLYGITGILLFNFCYLLFPNLIFSFVASMLFMFH
                     PIHTEVVANIKSIDEILSFMLCISACIFLIRYVQTKNIKSLVLTSVCFFAALLSKESS
                     ITFLAIFPLLILLKSDKKATSFNWSEVLKPMLTLAVFALLFLMIRHSVLSKIASGDPS
                     IADNLLMAAKSYDQKFATAVLILGMYLKLLFIPHPLVFDYSYNQIPLVGIGNMWFILS
                     LLIHTGLLIWAILIWKKNKLLSFTVLFYFISMSIYSNIFYMIGSSFGERFLYTASFGF
                     CMAVAYALSLISRNQNAENVVAFFKNNLTVLLILMPVAAAFAYKTITRNTVWKSNYTL
                     YANDVNLSPNSTRTHYYYGNLLAKDDFQKGKTDQEKDSIQKLALSELEKSVGIFPFGD
                     AYNQMGIIYDKRKDFKKSFESYQQAMKAAPNDPMIQNNLGSLYFNMGQIPEAKACYEK
                     AVQLNPSYSEAFMNLGSACGMMKLYDDAVTNYLKAIKFDPNLSQAYYYLGLTYRFKGD
                     QQNSEYYYNLAAKMDPEKYSRKK"
     gene            complement(23279..>23377)
                     /locus_tag="PKY09_07825"
     CDS             complement(23279..>23377)
                     /locus_tag="PKY09_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB12805.1"
                     /translation="LRTHLKRLNRRTICFTRSAIMLFACLKIYFWG"
BASE COUNT         7475 a         4336 c         3984 g         7582 t
ORIGIN      
        1 ggaccattaa cggtaaatat ccggcccgtc aggatgctat taatttctta ctcaaaacaa
       61 taaaacaaat tgaagtgcgc agccccgttg gtaaagctgc ccttcagaac gttttgaaag
      121 atttgagcag cacaggaaca aaaattgaga tttacagcaa aggcgataaa attaaaacct
      181 attacgttgg cgggccaaca caggatatgc ttggaacctt tatgtatctt gaaaattcta
      241 ccgtaccatt tgtgatgcat attccgggtt ttaacggtta cctcacaacg cgttattttg
      301 caagtgccga tgaatggcgc agccgcatta tttttcaata tggtgaagga cagattgcct
      361 caataatggt tcagaataac caacatcccg atgaatcttt cagagtaaaa agagacgagg
      421 atcattatgt ttactattca agcctgactg caactacacc ttccgaaatt tttcaatctc
      481 aattagttgc atacttagcc aaatatcagc ttataggatt tgaaaggcca acctacgacc
      541 ttgatgttga ctatcgcgat tcgttattaa aaacgcaacc tatcagaata cttgaagttg
      601 aaggtgtaga tggaaaaaaa gataaaattg aaatgttcct gaagccggtt gacacaggaa
      661 ccatgaccag tgttgatgaa agaacagggc ttttgcgcga gtttgaccct gacagaatgt
      721 atgcctcatg gaacaatgac accaatttga ttgtggttca gtatcatgta tttgacaagt
      781 tgtttagtaa accagaccgc attaaaggat ttggcggtct gtaaattttc ataactttat
      841 cctttctcaa agttacatct gcttatattt gccacctaac ttttaatatt tatgaaacta
      901 attgtttcca gccttgtact tcaaaaacat ttacaatcta tcagcggggt tttaaccact
      961 aactctcccc tgccgatttt agacaatttt ctgtttgccg ttaatggcag cgagcttacc
     1021 ctgtcggctt ctgaccttga aactaccata acaacaagac ttaaagttga atctaaagac
     1081 aaaggcataa tagccatccc aggtaaaatt cttcttgaca tcttaaagac ttttaatgag
     1141 cagccgctta cctttaccat caatgaaaaa aatcatacag tagaaatcag ctcagattat
     1201 ggaaagtata aacttaatgg tcagaatggt gaagactttc cacgcatacc ggttctcgaa
     1261 aaaagtaacc acattgaatt gccctcatca gttttgcatg aagcaataaa taaaaccatt
     1321 tttgctacgg gcaatgatga tttgcgccct gttatgtcag gtgtattttg tcagctttcg
     1381 caggacagca ccatttttgt agctacagat gcacatcgtt tggtgcgcta tcgcagaaca
     1441 gatattaaat caaaagaagc aggttctttt attttgccta aaaagccgct taatctgcta
     1501 aaaaatattc tacctcagga agaaacacgc gttaaggttg actataacga agccaatgca
     1561 tttttcagtt ttgataacta tgatttgatt tgccgtttga ttgatggccg ttatccgaac
     1621 tacgaagcag taattccggt tgacaatcct aatgttttga ctgtggacag agttgcattt
     1681 ctaaattcta taaaacgtgt ttctattttc tcgaacaaaa caacttatca ggtaaaacta
     1741 gcaattaaag gaagtgaatt agcaatttct gctgaagatg ttgattatgc caacgaggct
     1801 aatgagcgtt tgacttgttc ttatgatggc gctgatatgg acattgcttt taatgcacgt
     1861 tttcttgcag atatagtttc aaatcttgac actgaagaga ttcatctgca catgtcattg
     1921 tcaaaccgtg ccggattgtt aactcccgga aaagatcata ccaatgcagc agaagatatt
     1981 cttatgctca ttatgccggt aatgcttggc tgatgatttt ttttgccggt ttgtattgtt
     2041 aagaatgtaa atttttatgt gttacaatat tgcaataatg gaaacccggc agcagaaata
     2101 tgccgaacgc tataaggatg ttattggagc agccttcaac tttgaatctt ctcagcttga
     2161 actgccactt tactattttg tcagtggatt tgcacatccg tttttgccaa taatcaaaca
     2221 tgatggactt ttcttatttg aatggggact cattccttca tggataaaag atgctaaaaa
     2281 cgcctcggaa ataagagcta aaacactgaa tgccgttggc gaaacaattt tcgaaaaacc
     2341 atcattcaga aagagtattc ttacaaaccg ttgccttctt ggaatatcag gcttttatga
     2401 atggcgggat gtaaacaaaa aaaaataccc ttattttatt aaaacaaaac actcagaaat
     2461 attttctctt ggctgtattt atgaaacatg ggttgacaaa aataccggtg agatgcataa
     2521 taccttcagc atcattacca caccggcaaa ttcaatgatg gaaaaaatac ataacattaa
     2581 aaaaagaatg cctttaatca tctcaaaaga agatgaaagg aaatgggttg atccggtttc
     2641 tgatataagc gaaattaaat cgttgattaa accctatgat gcctcagaaa tgacggccaa
     2701 taccatttcg caggctgcta acagtgcaag agcaaaccgt aatgttccgg agattgtaaa
     2761 ccacgtgtca tatccggaac tgacttttct tgattgaccc tattcattca attattgccc
     2821 gaataaattg cgtacttctc ccctttttct ccttgaaact tcaatagctg tattgtcctg
     2881 aagcaataac atgttttcat tgttaaatga tctgataaat tttttattca ccaagtgagc
     2941 acggtgtact ctgataaaat caaaatccga aagcaaatca tcaaattctt ttaaagtctt
     3001 tgatgacaaa atggtatcgc tattactgca agttatcttt gtgtagttgc catcagcttc
     3061 acatcttata atttcttccg gaagcaggaa aaacgttttt aaagctgaat taactgcaag
     3121 tctgaatgaa tctgttgact gtgacgtata atttctgata aaattattaa tgacagactt
     3181 tctgtcgtag gcattttttc gtttgtcgag aaacttgttt atggcagact taaattcttc
     3241 tttattaact ggtttgagta agtaatccaa tgccgaaaac tttattgctt tcaatgcata
     3301 ttgatcatag gcagttacaa agattatctc aaaatcgtgt tcctgaatat tctcaagcat
     3361 ttcaaagcca cctttgtcag gcatttcaat atctaagaaa accaagtcag gtttgtgaaa
     3421 gtaaatttgc cttagtgctt cgacagcatc tgcagcttcg gctactaaat caacctgctg
     3481 aaaatacctt ttaagcagag atttaatttg ctctctgcca aacttttcat cgtcaacaac
     3541 aattgctttt atttctttca caaaacaaga gttctaaaaa cacaaaaata ttttaaccat
     3601 tcaatctatc ttattttttt gtttccattg atttaactta tctaccatct ctaagcattg
     3661 ctttcttatt tgcaaaataa ccggcatcag tttgttcttt ttaatactct ctctctgact
     3721 ctcaatttct gatatcttct gttcacagtt ccttaatcca acagatgcaa tcatgggttt
     3781 aatactatgt aataaaacaa taatctgttt aacctcattg ttctttaatg ccatttcaac
     3841 ctctctaaaa acctctggaa ttctttgcag gtaaatatca acataacgtg acatttcagt
     3901 aatatcgcca tcagtaaaat ctataagata ggacaaatca atatctgctg acttgtatgt
     3961 tttttcagta acttcactgc ctgcttcatc tttagggtta aatctaaacc ggtttacaac
     4021 atccaccaac tcctgaattt taaatggctt tgtcagatag tcgttcatgc cggccgaaag
     4081 acatttatcg tattcatgtt gagaaacatt agccgtaaga gcaactattg gaattttgct
     4141 ggtagcttca ttcaaatcat ttctaataat agcagtaagt tcgtacccat ccatctcaag
     4201 cattgatata tccattaaaa taagattaaa tctgcatgtt tgcaatgctg caatagcctc
     4261 taacccactt ctgacaatat gtagttttac ttcaggtatt tttttattaa gtgcatcttt
     4321 aaccagaatt tggttgtatt cattatcttc taccaacaat attgaaaaat ctttctttac
     4381 tgagtctgca ctaataggaa tatgttctgt ctttttagct tctggaatca aagctgattt
     4441 ttcaacatct atattgaagc taaaagttgt tccttgattt tccacactgc tcacgtcaat
     4501 atttccaccc atcaaccgga caagactctg tgaaatggtt aagccaagac cagttccgcc
     4561 atactttctg gtattgtaag cattcgtctg aataaacgaa tcaaaaattc gttcttgttg
     4621 attttttgga attcctatgc cgctatcaga aacttcaaat tgaagtctgc ataagtcttt
     4681 gttttgcgac tgaagagatt gtgtttttct aactgaaacg ataacaaaac cattatctgt
     4741 aaacttaata gcattgccaa caatatttat gagtacctgt gtgattcgaa atgggtcagc
     4801 aataataaac tccggaatat cttcactaac atcaaattta aaatcaatac ctttagtcga
     4861 ggccttctta ctgaaaacct catagacatg atccagtata tcctgaaatc taaatggtat
     4921 tttctccaac tccattttcc cggcttcaat tttcgataaa tcaagaatat catttataat
     4981 aaccagtaaa ccgtctgaag aatttttaat gatttgtata tatttctcgt ttagctcatc
     5041 ttgttttgtt tcctgcaaca gctctgtcat ccctataatt gcattcaacg gagtacgaat
     5101 ttcgtgactc atattagcaa gaaattgctg cttaaacctc tcactgctct cggcacgatc
     5161 tttctcgtat gcaatgattt tatttttttg ctctaacttc ttagctgttt cacgtttcag
     5221 tctgaatctg ttgtatatta ccaacagaat aagaaataac atcacagcac ctgcaataaa
     5281 aatattcttc aaaagtcttt gccattcggc attggctttt tcaattgact tgtctctgtt
     5341 gagcagttca atcattttat ccttctgctc ggtttcatat ttagtcttta agtcatctaa
     5401 cctgatttgc tgctgacttt cacgcgtact gtctttaatt gcagactcac gcttaaaaaa
     5461 atcaaatgcg tttttataat caccctttga agcatacaaa tcacttaatg agttgagcac
     5521 ctgactctcc aaagaagaaa atttgtttct ttcagcaatt tgcaatgcca tttgcatata
     5581 agtaatagct gaatttattt ttccttgctt ctgatagacg tttcctatca tgatgagctc
     5641 ataacacaca tctgccttgc ttccggactt ttcgaagttg gttcttgcca tctcaaaaaa
     5701 tttcaatgcc tcattgatcc ttccatgttc aagatgtagc gaaccaatac tctctaaagc
     5761 ataagcttct ccgctaacat tgcctaatgt atgatacatt gctgatgatt gattaagata
     5821 aaatgcagca ctgtctaatt gttttaaacc aaggtaggca tttcccaatc cgttaataca
     5881 atcagcaagt ttgttactgt cattgttcag aatattaatt tccatagcct gaccaaaata
     5941 tggaatagct attttaaatt ccttctgact attgtaaaca atgcctattg aatacaaact
     6001 tccgggcaac aaccatgatt cagggcatcc cttagaaagc tgtatgcact ttaaataata
     6061 ttctaaagca tctgccagct gattctgatt aaaatacata cctcccaagt tggacaaggc
     6121 cactgctgta tgacaacgat taccggtttg attaaaataa tttagggcaa gtaaaagaca
     6181 tttcttggct tcaacaaact cgccctttct ggtataacac catccaagac tgttataaca
     6241 gtcgccaatt cccttaatga atttaatgga aagtgccagt gcaagacctt tctttccaaa
     6301 atatatacca ctgtcggtat tggtagtact ataattaaaa cacaaatcac gataagcctc
     6361 tactcttgcc gaatcattag ctgcttgttt taagtcattc agatataaag gaatcttaga
     6421 agcatcctga ctcctgccag tcaggcataa caataaaaaa agaaatagat aagtaacgaa
     6481 acgtttcacc tatgtgtgtc gattttggca gcaatttagt gcaagatagt aattgatata
     6541 atatcaattg aaccgtttaa taaatttttc ttaccgctta ataattggaa gatgaatcat
     6601 ccgatatcac ttgctattat tgaaatcaaa ttcagaaaat aattgaaact aaatttaaat
     6661 caaaatgaaa aacttataca ttttattgtg gattctgctt actgcatata atacaaaggc
     6721 acaattcagc aacagccctg cacagcctct ttctgtttgc aactcaacag caacaaaaga
     6781 gaacttgcag attttacccg atggtagtgg tggctatttc accttttgga ttgacaacag
     6841 aaccggtact attaaaaaac tttacggtca gcatttggat gctgcaggaa atgaacaatg
     6901 gacagctaat ggcaaaacaa ttattcagca ccctacacag tctgtcaatg cttttcactg
     6961 tacgctattt aacaatggta tattgtttac gtgggtacaa agtggcgcat cgtatcagga
     7021 ttcgttgttg gtaaaaaaag taaattttag tggtaacgat gtttgggcac agccaaccct
     7081 tatagacaaa agtagtcagg ctgcaagtat ccttggtatt gaaacttatt cgatgaatgt
     7141 gatagccaat gactccgggg cgttcgttaa ctattcagta gtttactacg gtggttcctc
     7201 agggccaaag ttcaacagaa ttgatttcaa tggtgtaaaa caatggagta cttccaagac
     7261 aacctcctta cccggctata catatcttta ttataaaggg ccacagaata ctttttttag
     7321 tatttccaga ggcaatggtc ttggttcagg tatgtatatt cagaaataca acctgcaaag
     7381 cattccacag tgggcaaatg ctgtggatat ttcacaaggt gttaacacca atggctttgg
     7441 tggattgcag tacctcttta gcgacaatgc aggtaattct tacattgtat gggagagtat
     7501 gagtaataaa actctgttaa caaagcttga tgcagccggc agttttgtgt ggacacctca
     7561 atttaaagct acagtgggat tagcgaacac cggtcaaaac agacctcatg caactttcta
     7621 taacaacaaa ttatacatag tatggatgga caatagggtg gccaatcaaa ctacagttta
     7681 tgctcaggca tacaattcaa gtggcgcaac acaatggctt actgatggag tagagttagg
     7741 agaaaacaat tactattatt cctatccaaa gattgctgtc agtgatagta atgctgtact
     7801 tgtagcatat aataatccca gtttgcaatc catcgtaggt catcgaatca aatcaaatgg
     7861 caccttagcc tggccattat ccggaattga cttggcaact gaccactcat ggatatctta
     7921 taatagcatt acagctataa atgacaccaa tggatgtaat gcaattttct ggaatgaaag
     7981 tagcacaagt catatttggg gtgctaaaat ctgcagtaat ggtgttttgg ttaatactat
     8041 tgataattcc aaacctgatt ttgaaatttc accaaatccg gcatcagata atctgaatat
     8101 aaaacttgga agcattttaa actcaaccgc catcagcata attgacatga atggaaaaat
     8161 tatcatggag atgaatatac ctgagcccat taacgaatta aacctagact tgaaagggct
     8221 tgcaaaaggt atatatgctt tgagtattac atcaaaagat atccgccaaa caaaaaaact
     8281 gattattaag tagtctttaa ccaaaacaga aaaactccgg tttaatgccg gagttttaaa
     8341 tttaatcacg gccatgtcaa agcaacctcc aaaaattgat catgaaataa ttcgcgaaaa
     8401 gacattagac cgaattttat ttatactcga aattgttctg gttattactt taatagcatt
     8461 atgtggtgca attattactt cactatgaaa aaaaaatcat aaacaataat ctacaaagaa
     8521 tcattacatg attttacatt gcatgtttca agaggttatt aaaataaaat tataccttat
     8581 ttgatttatt tagaagtatt tgtttacttc tatttgcatt caattaaaat agtttatacg
     8641 tttgcaccta tcctaatgta gtttaacgga tacatggtag cattttttac atcgtatgca
     8701 cctgtaaccc ataaaaaggt tatattacat caacgggaaa ttgtttttgg aggcaatcaa
     8761 aattccgctg gcttgatggc aatgctttgc ctttttttaa aaaacaatag aattcaaaaa
     8821 atcttcgtaa agtttctaca accgtacgat tgttttattt attctgagtt ttggattaac
     8881 agattgcttc atatcaatca caaaatcctt cctcattcca cacatattaa ttacttaaat
     8941 taaaagttat gcaacaaaat tccattatgt tattttttat gctgcttgtt ggcattggca
     9001 caatcttttt tgtatcgata tttatgtctt cacttaatgc agataaagat tcaaccggag
     9061 taaaaaacct attaaaaaaa cgtttttggt tcaccttaat tcttattgtg gtgcttggca
     9121 tttttgctgc tgcaacttta ccaaaatctc catacttcac atttgccaat gaaacacctt
     9181 caaccgtgat tcatgttggc acgatgcaat tttcattttt tctttccaca aaacctattg
     9241 acccaaaaag tccaagtgga gaaactattg aaattcctca cgaccaactc gtagaattca
     9301 gagtaacaag ttttgatgtc aatcacggct ttgctgttta cgacccttca atgcgattga
     9361 ttacacaggt gcaggcaatg ccgggctatg tcaataacct tcgctataaa tttactgaac
     9421 ctggcaagta tcatattttt tgccttgagt tttgtggcac agggcatccg gttatgcaag
     9481 caacatttaa agttatttaa taaaatttta catcatgaat atagaatcac ttaaacttaa
     9541 aaaaataagc gcactttgga taatagtcat acttgtgctt tttatagcaa gcattgtact
     9601 tggaattctt atgaggctca atcagggaaa agtaattgaa caatcaccac ttacttttta
     9661 taccaacatg acaacacatg gtttaaccat gattggaatt tggtttgctg ccggaatggt
     9721 tgcagtaaac tatcttttgc aacgctatgt aaaaacaagt tatggtgtta acctgtttgc
     9781 tttcattctt actgttattg gtgttgtaat gctttggata actacctatg caggcaagtt
     9841 tagtaccggc tggacttttc tatatccttt accattcaga gatcttggtt ctgagagttg
     9901 gtcaactccg ttgtttctta tttcattaac tgtcttgggt gttggttggt tagtgtggag
     9961 tgttagcctg atggctcaga tactaaaaaa atatacgctt tcacaagctt ttgcctggca
    10021 gcattttaaa agcaatgcat cagtagaaac accaccattt atacttataa caactgtaac
    10081 attaattggt gtcattgcat cattaattgc tgcagtaatt gttatcatac tttattttgc
    10141 agagtatttt tctcatggag attttgtcaa tgatgcttta ttgatgaaaa acctgattta
    10201 cttcttcggc catactattg caaatgaaat gctttactta ggcttggctg ttatctatga
    10261 aatttttgga gaagtaagtg acagacctaa atggaaaatg acctggtata tttcattggc
    10321 atggaactgt gccataattt ttattttaac tgcattcttc caccatctat acatggactt
    10381 tgtacagcca caggccttgc aaattgttgg tcagttggcg tcttatcttg caagtattcc
    10441 atcagcagca gtaacagtat tcagtatagt tgcagctgtt tatggcgtta aggtaagatg
    10501 gtcaattgta aacctgttgt tttttctggg tgttgccggc tgggtaattg gtggtcttgg
    10561 ggctgttatt gatgcaacta tttcaaataa tataatgctg cataatacgc tatgggttcc
    10621 tgcacacttt cacacctaca atgcaatggg gaatgtttta ttcagtctcg gattcttcta
    10681 ttggttctcc acacagtatg ttgacaaatt agttttaagc aaattgacca aatacacttt
    10741 agcacttcta attattggtg gcattggctt tttaatggca ttctatgtgg ctggtgccaa
    10801 ttcaattcca agaagataca gtacttatcc ggttgaactc acatcaggac cctatcttgc
    10861 taaggttggt gccttgtttg cttcaattta tctgattgcc attttgattt tcttttataa
    10921 tattgctaaa cgatgtttcg ttattttatc tgcttcttca cgttagcatg ttttagcttt
    10981 tttgtttatg ctaacgataa tcactctgaa ttaagagaag agcagaatat ttacgggaaa
    11041 atttataatg cacctctgca cctatcagga agtagtaaag tcacttctgt tgaatcacta
    11101 tacagccgga agccattaat agtagcatta gtttttaccc gatgttacgg catctgcaat
    11161 ccgtatttac ttcaactgag tgaaaatata aaacaactac aaagcaatac tgattttaat
    11221 gtacttgttg taagctttga tccgagagat aatcttaagg acatgactaa tttggcaaaa
    11281 caatatagac ttgagaataa tcgccaatgg aactttgcaa caactgatag cattaatgaa
    11341 tttattaatt ccataggatt taatccgaaa tgggatagca ttacccaaca gtttgaccac
    11401 gatgcgttgc tggttggtgt aaatgaaaag ggcctgataa caaaaaattt gttaggcata
    11461 agaactgtag atgatttgca attgatgatc agaagtattc ataatgtgtt tattccctcc
    11521 taccgcttac caacaaaaag caatatattt tcttgcttta actacgatcc gaaaacacaa
    11581 accaacagac cgggtttggg gctattattt gttgcattgc ctgctataat aacttttctt
    11641 attgttatca ttatcgttat taaatcaaag agagcagctg cttaagattc ttgtacagaa
    11701 ttttttgtta cattttaata ctcctttgtg ccgtgtaaaa caaattaact tccaatgaaa
    11761 accataatca caataaattt gttttctgat aaatgatttt aaaattacaa cagctatgtc
    11821 ttcaataaaa attaaacgta tttatgaagc accggataaa agtgatggtt atagaatatt
    11881 ggttgacaga ctatggccaa gaggcattaa aaaggaatat gctgacattg atgaatggag
    11941 taaagatgtt gcgccatcat cagagctaag agaatggttt ggtcacgacc cggaaaaatt
    12001 taaagaattt acagcgcgct actcaaaaga acttcacagt aaaaaggagg tattaaaaca
    12061 tattgttgaa attgcaaaga agcaaaatct tactttactt tacggagcaa aagacaccga
    12121 acacaatcaa gctgttgttt tgcagaaagt actgaataaa atgttatgaa ctccaaacca
    12181 ataaaacgac ataaggcaat acaatccata agtagagaac attaccaagg tctgcttttc
    12241 tgctcaaaaa ttagaatggg tcttaaaaaa caaattgagc ctttgcgtat ggcaaaatat
    12301 gctaaatggt tttatgctga acatctcata ccccactttg aaatagaaga aaaatatttg
    12361 ttccctgttt taactccgga gaatgaaatg attcaaaaag caattaagga acatcgcatg
    12421 cttactgatt tgtgcaaagc agaggcagaa ctggcgacaa atgccaaact tgtaatgctc
    12481 gaaaaaacat tagaagcgca cattcggttt gaagaaagag tactttttaa agaaattgaa
    12541 tccattgcaa catcagacca acttgagttg ataaacaaac agcataaaga ggaaaagttt
    12601 gttgacaatt tagcagatca attctggaaa taatttcaac tcctaaactc aatgtacaac
    12661 atgttacatt ccaacataaa aacagaaaat attttaattg ccagaaatac acgtcctaca
    12721 tgtatgcgta ttttagttta taatttcctt gcaaaacaac atgctgcatt atctataaca
    12781 gagattgaga aacactttca gcacgcagac agaatcacca tttatcgtac tttaaaaaca
    12841 tttgaggaga aagggtttat tcacagtatc caggaagata atattacaaa atatacatta
    12901 tgtggctctg cttgttcaga gcaatcgcac tacgatcaac atcttcactt ctattgtaaa
    12961 gcttgcaaac agacaacctg taaaacagat atagctattc cacagaataa tattaaagga
    13021 tttaaaattg acgatattac attttttgca agaggtattt gtgaaaattg taataattca
    13081 actatgcaat agcattgcat tggcatctgc catatttttg tttccggaaa aatatctggc
    13141 atcatgcaaa agcacattta taattctgta ggcgaacaat cttgttgttc aattgaagat
    13201 aataataata agcatgtaca ttccaatgaa ttgaaagatt cacatcatga ggcaggctta
    13261 ttttccatgt acgcgccagg tttattttca ttagtacttc ttcttgcagg aatgcatttt
    13321 gattattgga atacattctt ctggtttaac ggatggatac gtttagcttg gtatatcata
    13381 gcttatattc ctgttggatt tcctgtaatc aagaatgcat atttaggaat caccaaagga
    13441 aatattttct ctgagttttt tctaatggca attgctactc ttggtgcgtt tgccataaaa
    13501 gaatatcctg aaggagttgc agtaatgtta ttttatactg ttggcgaatt acttcagact
    13561 ttagctgtaa aaagggctaa atcaaacatt aaaactttac tcgatcaacg ccccgaggtt
    13621 gcaactgttt taaaaaacaa caatgccgtt tcaatgtctg caagcgatgt tgcaattggc
    13681 gaaattattc ttttaaagcc cggagagaaa ttagcacttg atggtatatt gctttctgac
    13741 aaaggagctt ttaacactgc tgcattaacc ggagagagta tgcccgattc aaaattgaaa
    13801 ggggaattgg ttcttgccgg aatgattaac ctgaatactg ttgtacaaat taaagtcaca
    13861 gctgcttatg aagacagtaa aataagcagg atgcttgagc ttattcaaaa tgcatctgct
    13921 cagaaagccc aaacagagct attcatcaga aagtttgcaa aagtttacac tcccatagta
    13981 gttgggcttg ctgttgcaat cactttactg ccttactttt ttactcacga gtatattttt
    14041 cgtgaatggt tttacagagc tttaatattt ttagtcatct catgtccatg tgcattagtc
    14101 attagtattc ctctaggata ttttggtggc attggtgctg ccggccggaa tggcattttg
    14161 gttaaaggca gtaatttttt agatgtactt gcatcagtgc aacatgttat acttgacaaa
    14221 acagggacaa ttacagaagg tgtttttgaa gtacaggatg taaaaattat tcctgcagag
    14281 gacagagata aaatattaca gatggtcaat gcaatagagt ttcatagtac gcatcctgta
    14341 gctaccgcta ttcacaacta cgtaggcaat actacaacaa ttgaactgaa tcaagtagaa
    14401 gaaatttcag ggcatggtat gaaaggattc gttaacggca aagaattgtt agttggaaat
    14461 tttaagttat tatcgttgca taatgtatca tttgacattg atcctgtttc tataaaaaat
    14521 actgtcattg ccattgcttt tgatggcaaa ttttcaggct acataaccat tgcagacaac
    14581 attaaaccat ttgcagcagt cgccatacaa aaattaaagc agatgaatgt tgatgtgact
    14641 atgttgagtg gtgacaagtt aaccgttgtt gattcagttg ctgatcaaac agcgatttct
    14701 catgcctatg ccgaattact gcctgatgat aaagttgaaa aaataaaatc tatcatcaat
    14761 aaaatgcaaa ccgttgcttt tgtcggtgat ggtgttaatg atgcccctgc cattgccatc
    14821 agtcatgttg gaatagcgat gggaggatta ggaagtgatg ctgccattga agttgccgat
    14881 gtagtgattc aggatgacaa cccgattaaa attccattag caataaatat tggtaaacaa
    14941 acgaagaaag ttgtttggca aaatattacg ttggcctttg ccattaaagc catggtgtta
    15001 ttgttaggtg ccggtggtat tgctaccatg tgggaagctg tttttgctga tgtaggtgtg
    15061 gccttattag ctatcttaaa tgcaacgcga cttttgcatt ttaaatttaa atgatttttt
    15121 gaatcatact ggctattgac tactactgat tctaattgaa tcttgaaaat gcagcacaca
    15181 aagatgggaa ctccatcact atgcttccac tacatcctgc ataaatagat ttgttcctcc
    15241 cggaagaatt acatttaaac caatgcccta ataattgggg tgaaaggctt atgcgtcttt
    15301 gttaaccact cccatagcac agaatttgtc aattctattg tttaccctgt cggcagcaga
    15361 tacagcaagt aaggctccta actgaatatt aatttctttt tttacagtcg caaacatttc
    15421 ttcaggattg ctatgcgcac cacctgcagg ctctttaatg ataccatcaa tcaactttaa
    15481 agaaagcatg tctgttgctg tcaatttcaa agcattggcc gctttttctt tattatccca
    15541 attgcgccat aaaatacttg aacacgactc aggagagatt acagaatacc aggtgttttc
    15601 aagcatcaat accttatcgc caataccaat tcccaatgca ccaccggatg caccttcacc
    15661 aataatgata caaataactg gcaccttcaa aattgacatc tcatataaat tacgtgcaat
    15721 agcttctccc tgtccacgtt cttctgcttc aaggccggga tatgcaccgg gagtatcaat
    15781 aaaagtaaca ataggtttgt taaacttttc agcaagcttc atcaggcgca atgctttgcg
    15841 ataaccttcg ggattgggca tgccgaagtt tctatattgt ctcgacttgg tattgtttcc
    15901 cttttgctgg ccaataaaca taattgtctt tccgtcaaca gtaccaaaac caccaaccat
    15961 agctttatcg tctttcacat ttctgtcgcc atgcagttca ataaatgtat tgtcagtgat
    16021 tgcgtttata taatctaaag tgtagggcct gtcgggatga cggctaagct gtacgcgctg
    16081 ccacggagta aggtttgaat agatttgttt cttggctacc tgaatctttt cctctatctc
    16141 agataaaaga ttgtttacat caattttgcc tttagttcct atttgttttg tcttttccaa
    16201 ctgttcgtat aactcttcaa ttggtttttc aaaatcaaga taattggaca tgcgaatttt
    16261 atttgcaaag gcaaaagtaa cattatttga ttatccctga cgattgttga aacttgtcta
    16321 aaatttatct ttgcgggtac ttatgctaaa ttttccaaat gctaaaatca atctggggtt
    16381 atttataacc tctcgtttac aagacggata tcatacattg gaaaccttgt tctatcctac
    16441 caactggtgc gacattttag aaataacgga gaataaaaat tatagcaaat cacagcctga
    16501 agacagtttt catatatccg gattatcggt tgatggtcca accaatcaga acttatgttg
    16561 caaagcctat catcttctta aagataaatt caatttgccg cctatacata tttttctgca
    16621 caagcaaata cctacaggtg caggccttgg tggtggctca tctgatggtg cacacaccct
    16681 tctgctttta aacaatttgt ttggtttaaa tattagtcat gaaatactat cggagtatgc
    16741 attaaaatta ggatctgatt gcccgttttt tatttccaat aaaccatctt ttgctttttc
    16801 aaaaggtgaa atattgaaac ctgcaccggc tgttttaaaa ggaagttttt tggttgtgat
    16861 aaagccatct atatccattt ccactgcaat ggcctatagt caggtgaagc cagaaatacc
    16921 tgaaacaagt attgcagaaa ttttagaaat gccaaaagaa caatggcgtt attttttgaa
    16981 aaacgatttc gaaaaaacaa ttttaccgca atttccgcaa atagaaaaat taaagaccaa
    17041 cctctatgag gcgggagcgt attatgcttc tatgagcggc agtggctctg ctgttttcgg
    17101 attgttttat gatgaagtta aattatcttt tctgaaagat gaacagcatt attcaacatg
    17161 gctttaatta ctttttacgt ccaagcataa aggttccgta aagattaaaa aacacctgtt
    17221 tgttttttgc aaatgccata attgggattt ttttggtata agcatcgagc atcaccccaa
    17281 tttcaactgc agtaatacca tcattcataa tgttatattc aaacatcaca cctgacttta
    17341 aaaacaatcc gggatataat ttcaactcat ccaaaccttt ggtaaactct gccttcccat
    17401 aaatactttg cctgtcatga ttttgattgt taggatcgta tttttcaacc acacgttgcc
    17461 cattaccggt tgcaaaaatt aaatacacag gttttgtaaa gcctaaagac ggacctcctg
    17521 ttgaattcca tctgatttca aaatgtctgc gctctgcttt ttcaaataaa acacgttcat
    17581 agcaaaagcc accatgcagt actaataatg aattcatttt tccgaacaca aatgtctttg
    17641 gtcgctctga aagatagctt acagacttat actccttagg atgcttcatg ctcaccgctt
    17701 ctacttcaaa catccgtttg cggctaacag tcaggttttt tccttttcgg aacataaaac
    17761 cccagccact gctgtgcaag gtagcaccga tagagaattc attggtataa cgcaagggca
    17821 tatcttctac ataatcgtaa cgcacatctt gtgctagtac gctacatgat aacagaaaga
    17881 ataatgctgt aagccttaat tgcattgagc aaaaataaat tatttttatt tcagccgatg
    17941 aatcagtgca ttatcatgct gattattttt tcaatatcta cattactgat gaaaaactaa
    18001 ctttgtatca tgttcagaaa aacaaatgag gaaagcctga aagaggtaat tgagcaactg
    18061 ttggacacgt ataaactgcg tgacaaactc aatcaggtaa agcttatgca aagctgggat
    18121 aacataatgg gtgaaatgat cagtaaaaga acagaaaaac ttatacttaa agatcataca
    18181 ttgtatatct acctcaactc tgcaccttta aaagaggaac tcaactacgg tcgtgaaaga
    18241 attgttcagc tacttaacac agccctcgaa agcgatttta ttaaagaagt tgttatcaga
    18301 taagtgtttt ctctattagg cataaccttt agatagaaat caaaattcat ttttttacaa
    18361 ttgtcgctta aagataaact taaggaatca aacggaaatc attaatcaat gaaagccgtt
    18421 gaattcaggt aaaaagtaaa cttttatatc aatccatggc acccataaaa aacaaaaaag
    18481 ctgcctcttt ttgaagcagc ctttgttttg tgatcccgct ggggctcgaa cccaggaccc
    18541 ctacattaaa agtgtaatgc tctaccagct gagctacgga atcgggattt ttaataacga
    18601 gggcgcaaaa gtattgttat ccgttttaat taacaaaaga tttttgcttt tttattaaca
    18661 aaaaatggct ttttatgtaa aagttggatg tctttcaaat ggtttattgc ggtcaaaaag
    18721 atatctgtat gtaaagagtg tcctgcttcg ccatgaacct aacgactctg ccacagatac
    18781 catcttggga ttgaaatcgc ctatccattg taattcacaa tcttcgtact ttgttgcatt
    18841 gcgaatcaag gtggcccctt ctattatcat aaaactatct acaccctttc cctgaaattc
    18901 aggaattaca ccaaacacaa gccctatcat tcttttagaa actcttcgtt ttaacatcag
    18961 tataaaacgc aatttttcaa accagccaaa cttcccatta aaatatttga atatctgatt
    19021 gatgtcaggc aggttaaccc aaaatgctat tggctcattt ttataataca cataccatat
    19081 tattttttca tcaataacag gtttcatctt tttaaaaaat tgctgcactt gctttgattc
    19141 cagttctttg ccgctaccat gttttaccca tgctttgttg tagataattc tgaaatcttc
    19201 ggcaaacttt tccaattgag actttttaag atgtattgcc ttaaagtctt tatcggcatt
    19261 tatcaaagca tgacgctcgt aaaacttctc gtccagcttg ttttttactt ttaatccata
    19321 gcaatattgc ttgaaataaa cttcaaagcc ataagactca aaaaaatcct gatagtaaac
    19381 tggattataa ttcattttgt atggtggctc atgaaaacca tctgtaagca atccccacca
    19441 actgtcgcgt tcgccaaaat ttattggccc atccattgct tccatacctc ttgatgcaag
    19501 ccattcttta gctgtatcaa aaagtttaaa tgcaacttct ttatcatcaa tacattcaaa
    19561 aaatcccata ccacccgttg gctgtttgtt attctttgtt gccgtttttt tatcaataaa
    19621 agctgcaata cggccaacaa ccttatccct actgtttttt aaaatccaac gcgtacactc
    19681 tccatgtttg aaaaatttat ttttagcagc atcaaaaaca gattcaatat cattgtctaa
    19741 tggttgaatc cagttagcat ctgaggaata aatctgcttt ggaagcaata aaaaatcttt
    19801 cttatgtccg gcagtattaa cttcaataac ttgcataggc tgcaaaacta cttatttttg
    19861 ccatgagcat tttattcacc tttttcattg aaaaataaag ttgtcattat tacaggagcc
    19921 acatcaggaa taggttatgc ctgtgcacga tactttgcac aacaaggatg tcatattgtt
    19981 attgcagcaa gaaatgaaga gaaacttaaa cgtattggca gtgagttgaa atctctttac
    20041 ggcaattgtc ttgcagtgcc aacagatgta gcaatagaaa cagaatgtaa aaaccttatt
    20101 cagaaaacta ttgaaacctt tggctgtatt gatattctga ttaacaatgc cggcatttct
    20161 atgcgtgctt tattgaatga tgtttcaata aacgtgatgc atcaggttat ggatattaat
    20221 ttttggggaa cagttaattg cacaaaatat gctttaccct atctaataaa atcgcaaggc
    20281 tctgtagtag gtgtttcatc aattgcaggt aagaaagggc ttcctgcacg ttgtgcctat
    20341 agtgccagca agtttgctat ggaaggcttt ttggaatcag tgcgcattga aaatcttaaa
    20401 aacaatttac atgttttagt ggcagcaccg ggctatacag caagtaacat ccgcagtact
    20461 gcattaaata aagatggtca gcagcaggag gaatcacctt tggacgaatc taaattaatg
    20521 aagcctgaaa ccgttgccta cgaaatttac aatgctgttt tacatcgcaa acgcgacctt
    20581 gttttaacca ccaatgggaa actcacagta ttgcttaaca aattttttcc ggcttggatg
    20641 gataaaatag tttataattt cgtagctaaa gagcctgact caccatttaa ataatcaaat
    20701 aacaatgtca ataaaaacaa taattttctg taccctgtca tgtatcttac ttgcagcatg
    20761 caccaatacc aaacaacaat taaaagacga aatagactcg aaggaaaaac ttttatacga
    20821 aaataaaaat ggacgtataa acaccaaact ggctcgcgaa gtaatcatac attaccgcta
    20881 ttatgcagaa aaattccctg acgattcttt gtctgttgag tatcttttta aagcaggcga
    20941 tgtttctgtg ggtattgctg cctatgataa tgcattagag tattttaaaa tcatttcaga
    21001 gaaatatcca aaaagtaaca gagccgccta ttcgcttttt ctgcaaggat atattagcga
    21061 gaataacctg gaagacactt taagcgcaag gaaatattac aatgagtttt tggaaaaata
    21121 tcctaacaat gaaatggctg aaagtgcacg tttctcaatt ttaaaccttg gtaaaagtgc
    21181 agaagatgtg atgaaaaaaa ttgacggaaa caaagactca attcctgaaa aataactatg
    21241 gcaaaaagca aaaaacagaa agagagtatt gctgaagcac atcagaaaaa acaacagcaa
    21301 aaagctgctg aggcaaaaaa ccacaatatc aaaattcttc gaagaattct tgccttaata
    21361 tgcggattgt gtggtgtggt attatattta aacaccttgc atcatggatt tgtgttagat
    21421 gatttttctg ttattcttga aaaccgtatt accaaacaag gcttaaaggc aatacctcag
    21481 attttttctt cttcctatag aagcggatat tatttaggca ctgatgagct gtatcgccct
    21541 attccaaaat caatcttcgc catttcatgg aattggtttg gcgaaaatcc gctgccactt
    21601 cacttgttga atattgtact atatggcatt acaggtattt tattgtttaa tttttgctac
    21661 ctgctttttc caaatctgat attttctttc gtggcatcaa tgttatttat gtttcaccca
    21721 atacacacag aggttgtagc caacataaaa agtattgatg aaatactgag ttttatgctg
    21781 tgtatttctg catgtatttt tttaatcaga tatgttcaga caaaaaatat taaatcatta
    21841 gtcttaactt cagtctgttt ttttgctgct ctattatcca aagaatcatc cattactttt
    21901 ttagcaattt ttccgttgtt gattttatta aagtctgata aaaaagctac ttcattcaat
    21961 tggagtgaag tattaaaacc catgctgaca ctggcagtgt ttgcattgtt atttttaatg
    22021 attcgtcatt ctgttctcag taagatagca agtggcgatc catctattgc agataacttg
    22081 ttgatggcag caaaatctta tgatcagaaa tttgctactg ccgttttgat tttgggaatg
    22141 tatttaaaac ttctttttat tcctcatcca ttagttttcg attactctta taatcagata
    22201 ccattggtag gtattggaaa catgtggttt attctttctc tgctaattca taccggactg
    22261 ttgatttggg ctattttaat atggaagaaa aacaaactat tgtcatttac cgtacttttt
    22321 tattttattt caatgagcat ttattcaaac attttctata tgataggctc atcttttgga
    22381 gaacgatttt tatacactgc aagtttcgga ttttgcatgg ctgttgctta tgcactttca
    22441 ttaataagca gaaatcagaa tgcagaaaat gttgttgctt ttttcaaaaa caacctgact
    22501 gtccttctga ttttaatgcc tgtggcagct gcatttgcat ataaaacaat tacacgaaat
    22561 actgtctgga agtcaaacta cactttgtat gccaatgatg taaacctttc tccaaacagt
    22621 acaagaaccc attactacta tggtaaccta ttggcaaaag acgattttca aaaaggaaaa
    22681 acagatcagg aaaaagattc aattcaaaaa cttgcattga gtgaactgga aaagtctgtt
    22741 ggcattttcc catttggtga tgcctataat cagatgggaa ttatttacga taaaagaaag
    22801 gattttaaga agtcattcga gagttaccaa caagctatga aagctgcacc taacgacccg
    22861 atgatccaaa acaacttagg ctcattatat ttcaatatgg ggcagattcc tgaggcaaaa
    22921 gcatgttatg aaaaagcagt acagttgaat ccttcctact cagaagcatt tatgaactta
    22981 ggcagtgcct gcggcatgat gaaactttac gatgatgctg ttaccaacta tttgaaagca
    23041 ataaaatttg atcctaatct gtcacaggca tattactatc tcggtttgac ctatcgtttc
    23101 aaaggagatc agcaaaattc tgaatattat tataacctgg cagcaaaaat ggatcctgaa
    23161 aaatacagta gaaagaaata atttcttaaa atacctataa aataaaaagc tgcaatgtac
    23221 cacggttaat tttatctctt tttaagagca attgtgactt aacacgaaaa tatatcactc
    23281 acccccaaaa gtaaattttc aaacatgcaa atagcattat tgcactgcgt gtaaaacaaa
    23341 ttgttctgcg gttgagtctt ttcaagtgtg tacgaag
//
LOCUS       DAOQNP010000048        22649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_769_length_22649_cov_8.563645, whole genome shotgun sequence.
ACCESSION   DAOQNP010000048 DAOQNP010000000
VERSION     DAOQNP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22649
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_769_length_22649_cov_8.563645"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PKY09_07830"
     CDS             <1..515
                     /locus_tag="PKY09_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12806.1"
                     /translation="SERLKRQRHAEKAREQFLQSHDAINAANQEMRLLIDKFKKLKPL
                     IEDPTSHLHLGHSERPNPPMYELGTDGYYLCFYNSSPFNIGIVDNTLVNTGTLKVTLY
                     EKHGHYQINYSENVLKQVVLRFDRDLLGNNGWSDYDTGKNFMTTETLLDKWVKLFLDG
                     IGKRNINGRR"
     gene            complement(750..3314)
                     /locus_tag="PKY09_07835"
     CDS             complement(750..3314)
                     /locus_tag="PKY09_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNB12807.1"
                     /translation="MEAKFSPRVKDVITYSREEALRLSHDYIGTEHLLLGLIREGEGM
                     AIKFLKSLNVNLVELRKSVENAVKGSVNNVSNLNNIPLTKQAEKALKITYLEAKIFKS
                     DIIGTEHLLLSILKDEDNIATQILNQYGINYDIMREELETFKSNFKSEAPQSPSDDPY
                     SKDDDEGPGLGSGSTAKKSGDAKSKTPVLDNFGRDLTKAAEEGKLDPIVGRSKEIERV
                     SQILSRRKKNNPILIGEPGVGKSAIAEGLALRIIQRKVSRVLFNKRIVTLDLASLVAG
                     TKYRGQFEERMKAVMNELEKSPDVILFIDEIHTIIGAGGASGSLDASNMFKPALARGE
                     IQCIGATTLDEYRQYIEKDGALDRRFQKVIIDPTSVDETVEILNNIKDRYEEHHHVNY
                     TPEALKACVTLTNRYITDRFLPDKAIDALDEAGSRVHLSNIHVPENIVDLEGKISEIK
                     SEKNRVVRSQKYEEAARLRDSEKQLIDQLEAAKKQWEEDTKNQRYAVTDEDVAEVVSM
                     MSGIPLQRVAQAETARLIRMPEELKGKVIGQDDAIKKVVRAIQRNRAGLKDPNKPIGS
                     FIFLGPTGVGKTELAKVLATYLFDTEDALIRIDMSEYMEKFAVSRLIGAPPGYVGYEE
                     GGQLTEKVRRKPYSVVLLDEIEKAHPDVFNLLLQTLDDGQLTDSLGRKVDFKNTIIIM
                     TSNIGSRQLKDFGTGVGFATGAKNAQFDEHARGVIENALKKAFAPEFLNRIDDVVIFN
                     TLSKEDIIKIIDIELEKLYSRIAGLGYKLKLNDEAKDFLADKGNDVNYGARPLKRAIQ
                     KYVEDMLAEEIIKAELQEGDNIEVVFDKEAHVLKANISKSSPPPPKSKKKKEENNS"
     gene            3619..6177
                     /gene="gyrA"
                     /locus_tag="PKY09_07840"
     CDS             3619..6177
                     /gene="gyrA"
                     /locus_tag="PKY09_07840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNB12808.1"
                     /translation="MSEGEKIVRINIEEEMKTAYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLYGMLDLGVLSNRPYKKSARIVGEVLGKYHPHGDASVYDTMVRMAQEWSLRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLKKIAEEMLADIDKDTVDFKLNFDDTLQEPTVLPA
                     KIPNLLVNGAAGIAVGMATNMPPHNLTEVCNAVIAYIDNRDITVEEMLQYVKAPDFPT
                     GGIIYGYSGVKEALLTGRGRVVMRAKANIETLDNGRERIIVTEIPYMINKAEMIKKTA
                     DLVNDKKIEGITDIRDESGRDGMRIVYEIKRDAMASVVLNNLYKHTPLQTSFGVNNIA
                     LVNGRPLLLNLKDLIHYYVEHRHDVLVRKTKFELAEAEKRAHILEGLLIALDHLDEVI
                     ALIRASQTPDEARTGLIEKFALSEIQAKAILDMRLQRLTGLERDKIKEEYAELMKLID
                     YLKKVLGSEELRMLLLKKEVAEVLEKYGDERRTEIVYAADDISMEDMIEDEAVVVTIS
                     HMGYIKRTPLTEYRTQNRGGRGSIGSSTRDEDFVEHLFVATNHNYILLFTQKGRCFWL
                     KTYEIPEGNKQFKGRAIQNLISIEPDDKVFAFINVKTLEDEEYINNNFIVMCTRQGVI
                     KKTPLEAYSRVRQNGINAIVINDGDELIEAKLTNGNNEILLAVKSGRAIRFNENAVRT
                     VGRNSIGVRGVSLDHAKDEVIGMVCIKNAKEETILVVSEKGYGKRSDLEDYRVTNRGG
                     KGVKTMNITEKTGDLIAIKSVKEDDDLMIINKSGITIRMEVKDLRVMGRNTQGVKLIR
                     LDDDDSIASVAKIDMEITGNGNHKENGNDTEENPVTDTDKQNNNQTEENNSQPE"
     gene            6202..7326
                     /locus_tag="PKY09_07845"
     CDS             6202..7326
                     /locus_tag="PKY09_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12809.1"
                     /translation="MKRILTAVFISLALGSYAQRAQVQSAYSYLKYEQLDKAKESIDI
                     AVTNENTMNYDKAWYYRGLIYQALYKNEKFGNLSSDPLNEALRSYNKALELNPKFEYA
                     DDITEKKKILAQQFADMGYVNYNLKNFAGALSAYEGVVAIMPNDTSSYFNAALCAERA
                     GNTTKAKQFYTKLDEMQYKDAKMYHSYAQLYLTEGDTTKALEILSRGLSRFPEEKSIL
                     ITQTNIYISSGKTAEALNAINMAIEKDSTNANLYFAKGTLVEKTEKSKDAAIAAYKKA
                     IELKADYFDAYYNLGALYFNDAAHLANEANSIKDNNQYAKAKAVFEAKFKEAKPYLEK
                     AWELNPKDQSTMISLKQLYATINDTVNYAKVKAALDAETK"
     gene            complement(7398..7898)
                     /locus_tag="PKY09_07850"
     CDS             complement(7398..7898)
                     /locus_tag="PKY09_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNB12810.1"
                     /translation="MKNVANSVDDILKNVQEDRKEVFQKLHQVILKNLPKGFEAAISY
                     GGLGYVVPHSVYPDGYHCKPAEPLPFAGIASQKQSINFYHMGMYSDPDLLKWFVSEFP
                     KHSKQKLDMGKSCVRFKKMDDIPYKLIGELMKKMTVKEWILKYETAYKPKAKVVKVDR
                     EPKRGK"
     gene            complement(7933..10767)
                     /locus_tag="PKY09_07855"
     CDS             complement(7933..10767)
                     /locus_tag="PKY09_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12811.1"
                     /translation="MRIFLTAFLFFISGYFAGAQNVISGVIKDGSSGETLIGATVVIK
                     GTTEGAATDIEGKFSFETPRGYPLVLVASFVGFLNQEITLNGAQPLTIKLKKNEVLLK
                     NVTVTGSRISEKQKESPITVEALDYIAIKETPAAGFYEGLGQLKGVDLTSASLGFKII
                     NTRGFNSSSPVRSLQLIDGVDNQSPGLNFSLGNFLGSSELDVQKVDLIVGASSAYYGP
                     NAFNGVIALTTRSPFESPGLQFMVKIGDRKLNEFALRFAQVFKNKKGDNKFAYKLNFY
                     RFTALDWKADNYSPTEQSRNTSYNPGGYDAVNVYGDEDINGTDYKNFPGAFPGLMSFN
                     RKGYREEDLVDYNSNNTKAGLALHYMITPMVEVIASSNYGTGTTIYQGDNRYSLKNIK
                     FLQNRVEVREKDKWFVRAYSTSEDAGQSYDAYFTALLLQRSAKSDGHWKNDYENFWNG
                     NYNLSYIRKLPGFPPPPGFGPAYVQWLQSVNPFLLNNYYDSLVYYHQQAQAYASGVGN
                     PQNQNLAFFEPGTARFDSAFNAITSRKSYSEGGSRFYDKSKLYHIQGEYHYSFKWVEG
                     VVGGNYRLYKPNSDGTIFSDTSYVHYNISANSDTLSIDTLRNKLSVYEFGVYAGLEKK
                     ILKDKLKLNATVRLDKNQNFNLLVSPAGSVVYTPNNNHVIRVSFSSAVRNPTLTDQYL
                     FYQVGRATLLGNINGYTNLLTPESVTEFYDSSKSSFKFLNFFNVDPVRPEKVKTIEAG
                     YRATLFNRLYVDLNGYYSWYHDFIGYQIGVDADTFSINTFGGTIYDLVFNRVVRVASN
                     AQSEVTTQGVSVGLNYYIGKYFAFTGNYSWNKLNKKGTDDPLIPAFNTPENKYNIGFN
                     GRDIKGVGFNINYKWVQGFKYEGSPQFTGEIPSYDMVDAQVNYHLKDAGTTFKLGAMN
                     LFNNKHYEIYGGPKVGRMVYFSILFEVEKL"
     gene            10899..12425
                     /locus_tag="PKY09_07860"
     CDS             10899..12425
                     /locus_tag="PKY09_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNB12812.1"
                     /translation="MANPLTLAELESIAFKRKPVKVNDDIIKSINASFDFLVRFSKDK
                     IIYGINTGFGPMVQYKVENHELNQLQYNLIRSHSSGTGAVLNEVYARAVVAARLNNFL
                     QAHSGVSPEVISLLTTMLNENIVPEIYEHGGVGASGDLVQLAHLGLNLIGEGYVYYDG
                     KRQKTADVFKKLNIKPLTLQLRDGLGIINGTSCMTGIAAINLVYAKRLTRWMITLSAV
                     INEIIESFDDSFSAELNHVKLHNGQREVAQQMRDFLKGSKLVRNRKELFSDHAELSRT
                     EFKNKIQEYYSIRCVPQIIGPIYDTCMAAIAVTENELNSTNDNPVVIPEADNVFHGGN
                     FHGDYISLEMDKLKLAISKLSVLAERQLNFLLNKKLNRKFPPFLNKKKLGFNFGLQGM
                     QFLATSTTAENQALSNSVYVHSISNNNDNQDVVSMGTNSAIMTRKVIDNSYEVVAVLT
                     LAVCQAIDLLNVEEFQKLCKNTLSLHQLIRKHVAVVEDDVPQFENIRKVIDILKENQI
                     "
     gene            12429..13160
                     /gene="fabG"
                     /locus_tag="PKY09_07865"
     CDS             12429..13160
                     /gene="fabG"
                     /locus_tag="PKY09_07865"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNB12813.1"
                     /translation="MNCALVTGASRGLGKAIALQLAADHKLHILINYASNKQAAEETL
                     TIIKNSGGSGELLPFNVQDHETVNAALDRWHKENEDKFISVLVNNAGITRDGLLMWMP
                     EKDWDDVLNISLKGFYNVTRYVVNPMLRKRAGRIVNIVSLSGLKGNAGQTNYAAAKGA
                     IVAATKSLAFEVAKRKITVNAVAPGFIRSDMTEGLNEKELSKLVPMERFGTPEEVAHV
                     VSFLTSDKATYITGEIINVNGGIYS"
     gene            13164..14384
                     /locus_tag="PKY09_07870"
     CDS             13164..14384
                     /locus_tag="PKY09_07870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNB12814.1"
                     /translation="MQRVVITGIGIYSCIGKNIAEVTESLRQGKSGIIFDQQRKDLGF
                     RSALTGFVETPDLKDFLHRRDRLGMHQPAMYAFMSTREALAMAMLDADYMDKNETGII
                     FGNDSTAGSVVETIDKARKKKDNTLIGSGDIFQNMNCTVNMNLATIYKLKGINFTLSA
                     ACASGSHAIGMGYLLIRQGLQERVICGGAQEINPPAMTSFDGLGTFSSRENEPEKASR
                     PFDKSRDGLIPSGGAATVILESLESAQRRGANILAEVTGYGFSSNGDHISNPDFNGQE
                     RSLNMAMKLAGINASNIDYVNAHATSTPAGDGIEAKVISHVLGNNVPVSSTKSMTGHE
                     CWMAGASEVVYSMLMMQNDFVAPNINFEEPDDDTKTINIITTAKEHRINCFLSNSFGF
                     GGTNSALIVQRFRN"
     gene            14476..16392
                     /locus_tag="PKY09_07875"
     CDS             14476..16392
                     /locus_tag="PKY09_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNB12815.1"
                     /translation="MSVGDKLHEILIKYWGHSRFRPLQQEIIESVVGGHDTLALMPTG
                     GGKSICFQVPGIYLDGICLVISPLISLMKDQVDNLRSRGIKASAVVSGMSKTEIEIAF
                     DNCIFGKSKFLYLSPERLTSSLALTKLREIKINLIAVDEAHCISQWGYDFRPSYLRIV
                     ELREAFPEVPVIALTASATKSVRNDIAEKLNFKNHKFFQKSFERKNLNYIVEYNKDKL
                     TRTAEILKSVKGSAVIYVNSRGKTKDITTYLNSQGIKADFYHAGLSAADRNKKQSDWM
                     QNKIQAMVATNAFGMGIDKADVRTVIHADLPDSPEAYYQEAGRAGRDEKNAFAVLIYN
                     SADKADLELRFQNAFPEIKLIRLIYHALGNYLKVALGSGENISFNFDISDFASTYNFN
                     PLVAINALKIIAEAGYIHLSDAVYIPSRLKFTVGQAELYNFQVQHPESDKVIKTILRS
                     YGGSFDNFIRISEAELAKRLNIAQDVLIRHLFFLQKNELLEYVQQTDKPQLTYLISRV
                     KQETVVPDLKLLEFRKQRFKERVNAMLHYAESLTSCRSMMLLNYFGETTNTRCGTCDY
                     CRKRNKIELNDLTFEKQNAEIKEIIQQQTLRYDELLEKFPASKKEQYVQSLQWLIDSG
                     KIIISESDILLWHE"
     gene            16385..16975
                     /locus_tag="PKY09_07880"
     CDS             16385..16975
                     /locus_tag="PKY09_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNB12816.1"
                     /translation="MSKLHQSFYLQESAVDVAKQLLGKELCTKINGKLTSGIITETEA
                     YMGVNDKASHSWNNRRTHRTEIMYHEGGVAYVYLCYGIHSLFNVVTNLKDVPHAVLIR
                     SIIPKRGIEIIKKRRPVNKLMKQIASGPGMVSQALGIDCSMTGLSLFGDKIWIENTDL
                     QLSATQITATTRIGVDYAGADALLPYRFFINPEISN"
     gene            16975..17670
                     /gene="mtgA"
                     /locus_tag="PKY09_07885"
     CDS             16975..17670
                     /gene="mtgA"
                     /locus_tag="PKY09_07885"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNB12817.1"
                     /translation="MKNLFIRIRKILWKIIKWFFILSITGVILFSFVPVPFTPLMIIR
                     FVEQVFDSEREIQFRKDWEPIANISPLLVKAVMTSEDQRFNEHFGLDMEAIQKAVQYN
                     EKHKNKKRGASTISQQTAKNVFLFPSRSWLRKGFEVYFTFLIEIFWSKQRIMEVYLNV
                     IELGNGIYGAEAASQYYFHKPANQLSVHQAASLAAILPLPLKWSPTKPNARVVRRTEW
                     IKRNMKYAEGLEE"
     gene            complement(17699..17953)
                     /locus_tag="PKY09_07890"
     CDS             complement(17699..17953)
                     /locus_tag="PKY09_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12818.1"
                     /translation="MQILFVSFFKPKWRIISAFASGIYYGIAGVQYGLKKTSGSSEKF
                     TLWTDLFIFVILFIYSIRSIEIKHSTAILPSYNEVAITHF"
     gene            complement(18394..18480)
                     /locus_tag="PKY09_07895"
     tRNA            complement(18394..18480)
                     /locus_tag="PKY09_07895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18444..18446),aa:Leu,seq:taa)
     gene            complement(18578..18650)
                     /locus_tag="PKY09_07900"
     tRNA            complement(18578..18650)
                     /locus_tag="PKY09_07900"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18615..18617),aa:Gly,seq:gcc)
     gene            complement(18738..21323)
                     /gene="mutS"
                     /locus_tag="PKY09_07905"
     CDS             complement(18738..21323)
                     /gene="mutS"
                     /locus_tag="PKY09_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNB12819.1"
                     /translation="MQQYNAIKAKYPDALLLFRVGDFYETFSEDAVKTSKILGIVLTK
                     RSNGAASEMELAGFPYHALDTYLPKLVRAGQRVAICDQLEDPKTTKTIVKRGVTELVS
                     PGVTYNEKILENTHNNFLAVVHLTEDVSGVAFADVSTGTFFTARGKDAYIEKLLQNFK
                     PSEVVLQKSMQKDFVARFGDGFYTYTLDQWAFQKEYAEEVLLKHFQTNSLKGFGIHQM
                     DEMIVACGVALHYFGQMQQQTLSHITGIARIDEEQYVWLDKFTIRNLELVASPHENAR
                     TLVDVIDRTVTPMGARLLKRWILLPLKDIQTIQERHDAVAAFTLDESLRVQVATSLRH
                     IGDIERMLSKIALRRISPREMVQLKRALSQIQPIKDNLAAVGNSTFNVLSEQLNPCNL
                     LIQKIQDALVEDAPPLASKGGFIAKGFNQQLDDLRQVAFSGKDYLLKIQQREVEQTGI
                     SSLKIAFNNVFGYYIEVTNAHKNKVPESWIRKQTLVNAERYITPELKEYEDKILGAEE
                     KIATIENEIFQEIISFTQNYIQPLQVNASVLSQLDCLLSFANVALDNNYTRPVVNDSL
                     VLSITEGRHPVLEKQMPPGEAYIPNSIFLDQQTQQIIMITGPNMSGKSALLRQTALIV
                     LMAQTGSFVPAKSAEIGWVDKLFTRVGASDNISSGESTFMVEMNETASILNNITQRSL
                     VLLDEIGRGTSTYDGISIAWSIADFIHESKHARAKTLFATHYHELNEMTNTYSRIKNY
                     NVAIKEAGNKILFLRKLVPGGSEHSFGIHVARMAGMPPHVINKAEKMLQLLEKAHSNT
                     ELLRNAGHQADKDAMQLSFFQLDDPLLEQIRDEILKTDVNTLTPVEALFKLNEIKTLL
                     SKNKK"
     gene            21372..21908
                     /locus_tag="PKY09_07910"
     CDS             21372..21908
                     /locus_tag="PKY09_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNB12820.1"
                     /translation="MKKLTGSEMGRASKEGFKQIQKIPVVIILENIRSALNTGSVFRT
                     CDAFPVEKIILTGITAQPPHKDILKTALGATESVNWQYYSDIKDCFTELKAKGYKIFA
                     AEQTSQSIAMPDFKPNSDEKLALVFGNEVDGVSNDTLAMCDGAIELPQHGTKHSLNIA
                     VCAGIMIYDLFQKMKAAF"
     gene            complement(21905..22045)
                     /locus_tag="PKY09_07915"
                     /pseudo
     CDS             complement(21905..22045)
                     /locus_tag="PKY09_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011145223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            22085..>22649
                     /locus_tag="PKY09_07920"
     CDS             22085..>22649
                     /locus_tag="PKY09_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12821.1"
                     /translation="MGLTDVAAQEGYYDSLDADMQYWAQRQVYELLLSDDSLMLQGTA
                     ADTLLALWKDSVATGNIGAFRTVADSAIANAAYAAELNYNITPLNHAEENEQAVYALY
                     FALAGEQPDSAQHETLSAIAAENAISGGGGVYMARAMIDTSADDVTGIASLRMIAPST
                     NKNINKTGKLYPNPANTVVYYENELAAD"
BASE COUNT         7091 a         4411 c         4440 g         6707 t
ORIGIN      
        1 gatcggagag attaaaacga caacgacatg ctgaaaaagc tagagaacag tttctccaaa
       61 gccatgacgc tattaacgct gcaaatcagg aaatgagatt attgattgat aaattcaaga
      121 agcttaaacc tttaattgaa gacccaacat ctcacttaca tttaggacac tctgaaagac
      181 ctaatccgcc aatgtatgag ttgggcactg acggttacta tttgtgcttc tacaatagtt
      241 caccattcaa tattggtatt gttgataata ctcttgtcaa tacaggaaca ttgaaagtaa
      301 ctctttatga aaaacacggg cattatcaaa taaactattc agaaaatgtt ctaaagcaag
      361 ttgtgcttcg ttttgacaga gaccttttag gtaataatgg atggtcagat tacgatacag
      421 gaaagaactt tatgactacg gaaactctgc ttgacaaatg ggtaaaactc tttcttgatg
      481 gaataggtaa acgaaatata aatggcagac gatgaaattg atactgcaat tcttcgaaca
      541 gaaagcacca catgtagcta acacaaagtc aaccttgcac aacccaacaa aagagtaaaa
      601 agggatgtaa aacgacatca ttagctgcct tgtaacaaaa cggctgtctt tattgccggt
      661 ttgtattgag ttccgaacca attgcaccgt aattgcaaca aacaaatatt aaaaacaaag
      721 agccgctgaa aattacgcgg ctctttgttt taagaattat tttcttcttt ctttttttta
      781 gattttggtg gcggtggcga agacttggaa atattagctt ttagcacatg tgcttcttta
      841 tcaaaaacca cttctatgtt atcaccttcc tgcaattctg ctttgataat ttcttctgca
      901 agcatgtctt cgacatattt ctgaattgca cgcttaagcg gacgagcacc atagtttaca
      961 tcattgcctt tatctgcaag aaaatcttta gcctcatcat taagttttag tttataacct
     1021 aagcctgcaa tgcgcgaata cagtttctcc aattctatat caataatttt gataatatct
     1081 tctttgctga gcgtattaaa tattaccaca tcatcaatac gattgaggaa ttcaggtgca
     1141 aaggctttct ttaaagcatt ttcaataaca cctctggcat gctcgtcaaa ttgtgcattc
     1201 ttggcacctg tagcaaaccc tactcccgtt ccaaaatctt tcaactgacg cgaaccaatg
     1261 ttacttgtca tgatgatgat tgtattttta aaatcaacct tgcgacctaa tgaatcggta
     1321 agttgtccgt catctaatgt ttgtaataac aggttaaaca catccggatg tgctttttca
     1381 atttcatcaa gcaaaacaac tgaatatggc ttgcgtctta ctttttctgt aagctgtccg
     1441 ccctcttcgt atccaacata gccgggaggt gctccaatca aacgcgatac agcaaatttt
     1501 tccatatatt cactcatgtc aatacggatt aaagcatcct ctgtgtcgaa caagtatgtg
     1561 gcaagtactt tggcaagttc tgttttaccc actcctgtag gacctaagaa aataaatgag
     1621 cctattggct tgttagggtc ttttaatcct gcacgattac gctgtattgc tcgcacaact
     1681 tttttaattg catcatcctg tccaattact ttacctttca attcttcagg catacgaatc
     1741 agtcgtgctg tttctgcctg tgctacccgt tgcaatggaa taccgctcat catcgaaact
     1801 acttctgcaa catcttcgtc tgttacagca taacgttgat tttttgtatc ctcttcccac
     1861 tgttttttag ccgcttcaag ctgatcaatc agttgctttt cggaatcgcg aaggcgtgca
     1921 gcttcctcat atttctgact gcgtaccacc ctgttcttct cggattttat ctccgaaatc
     1981 ttcccttcca gatcaacaat attctcaggc acgtgaatgt ttgaaagatg cactcgcgaa
     2041 cctgcttcgt ccaatgcatc tattgctttg tccggcagaa aacgatcggt aatatatctg
     2101 ttggtaagcg ttacacaggc tttcaatgct tcaggagtat aatttacatg gtgatgctcc
     2161 tcataacggt ctttaatgtt attgagaatc tctacagtct catcaacaga agtaggatca
     2221 ataattactt tctgaaaacg tctgtccaag gcaccgtctt tttcaatgta ctgacggtat
     2281 tcatccaatg ttgtagcacc aatacattgt atttcgccac gagccaatgc aggcttaaac
     2341 atattggaag catcaagcga gccgctggcg ccaccggcac caatgatagt atgtatttca
     2401 tcaataaaca atattacatc gggcgatttt tccaattcat tcatcaccgc tttcattcgc
     2461 tcttcaaact gaccacgata ttttgttcct gcaactaatg atgctaaatc aagtgttaca
     2521 attctcttgt taaaaagcac tctggaaact tttcgctgaa tgatgcgcaa ggcaagacct
     2581 tcggcaatag cggattttcc aacaccgggt tcaccaataa gaatagggtt atttttcttt
     2641 ctgcgagata gtatttgcga aactctttct atctccttgc tgcgtcctac aattgggtct
     2701 aactttcctt cttcggctgc cttagtcaga tcgcgaccaa agttatcgag caccggagtt
     2761 tttgacttgg catcaccgga ttttttagct gtagatccgc tacccagtcc cggaccttca
     2821 tcatcatctt tgctatatgg atcgtcagat ggcgactgtg gtgcctctga tttgaaatta
     2881 gatttgaagg tctctaactc ttcgcgcata atatcatagt tgataccata ctgatttaat
     2941 atttgtgtgg caatattgtc ttcgtctttt agaattgaaa gcagcaaatg ttcagtaccg
     3001 ataatatcgc ttttaaatat tttagcttca agataggtga tttttagggc tttttctgcc
     3061 tgcttggtta gtgggatatt gttaaggttg ctaacgttat taacacttcc ctttacggca
     3121 ttctcaaccg acttgcgcaa ttccactaag tttacattga gcgattttaa aaacttaatt
     3181 gccataccct caccttcacg gataagtccc agcaacaaat gctctgttcc gatataatca
     3241 tgacttaatc gcagggcttc ttctctacta taggttatca catcctttac tctggggcta
     3301 aattttgctt ccatattctg tgtatttatt caattttaac ggtaaaaatc cgaaatagtt
     3361 ttcttttact tctgatgcaa tttttttaat tgtgaacaac aaacttaaca atattttttg
     3421 tttttgcatt gttagtaaat cttcataaca ttttatgagg attttatcat aaaatatgcc
     3481 aataaaacac taccttcgca gacatgacaa aacaagcaaa aaaaggcatt ttaacatgac
     3541 atgtttaccc ttgcccgttt aagctttttc actcgtaatt tcaaataaat ttcagaacaa
     3601 atattgtatt aacacactat gtcagaagga gaaaaaatag ttcgcattaa cattgaagaa
     3661 gaaatgaaaa cagcctacat tgattattca atgtcggtaa ttgtgtcacg tgccttaccc
     3721 gatgtgcgtg atggctttaa acccgttcac cgcagggtac tttacggcat gctcgacctg
     3781 ggcgtgttat ccaaccgtcc ttataaaaaa tcagccagaa ttgtcggtga ggttttaggt
     3841 aaataccacc cgcatggcga tgcttctgtt tacgacacca tggtgcgtat ggcgcaggaa
     3901 tggagcttgc gctacccatt ggtagatgga caaggtaact tcggaagtat tgatggcgac
     3961 agccctgctg ccatgcgtta cactgaagca cgtttgaaaa aaattgcaga agaaatgttg
     4021 gcagacattg ataaagacac tgttgatttt aaactgaact ttgacgatac actacaggag
     4081 ccaacggtat tacctgcaaa aattccaaat cttttggtta atggcgcagc cggtattgca
     4141 gtgggtatgg ccaccaacat gcctccacac aatttaacag aggtatgtaa tgcagttatt
     4201 gcttatattg acaacagaga tattacggtt gaagaaatgc ttcaatatgt aaaagcacct
     4261 gattttccta ctggaggaat tatttacgga tactccggtg tgaaagaagc tttgcttaca
     4321 ggaagaggac gtgtggtgat gcgcgcaaaa gccaacattg aaacccttga caatggacgt
     4381 gaacgcatta tcgttacgga aataccatac atgattaaca aggctgaaat gattaagaaa
     4441 acagctgacc ttgttaacga taaaaaaatt gaaggcatta ctgatataag agatgaaagc
     4501 ggacgtgatg gcatgcgcat agtatatgag ataaaacgtg atgcaatggc cagcgtagtg
     4561 ctgaacaatc tatacaaaca tacaccattg caaacttctt ttggtgtaaa caatattgca
     4621 ttggtaaatg gtcgtccgtt gttgctcaac ctcaaagacc ttatccatta ctatgttgaa
     4681 caccgccatg atgtgctggt tcgtaaaaca aaatttgaat tagccgaagc cgaaaaacgt
     4741 gctcacatct tagaaggctt actcattgct ctcgatcatt tagacgaagt tattgcattg
     4801 atacgtgctt cgcaaacacc tgatgaagcc cgcaccggat tgatagaaaa atttgcactc
     4861 tccgaaattc aggcaaaagc tattttggat atgcgcctgc aacgtttgac aggtcttgag
     4921 cgcgataaga ttaaagaaga gtatgcagag ttgatgaaac ttattgacta cctgaaaaaa
     4981 gtactgggta gtgaagagtt gcgtatgctt ttattgaaaa aagaagtagc agaagtttta
     5041 gaaaaatatg gtgatgaacg caggacagaa atagtttatg ctgcagacga catcagcatg
     5101 gaagatatga ttgaagatga agctgtggta gtcactatct ctcacatggg ttatatcaaa
     5161 cgtacaccgc tgacagaata cagaacacag aaccgtggtg gaagaggttc tattggcagc
     5221 agcacacgtg atgaagattt tgtagaacac ctctttgtag ctaccaatca caactacatt
     5281 cttctgttta cacaaaaagg acgttgtttc tggctgaaaa cgtatgaaat tcctgaaggc
     5341 aacaaacagt ttaaagggcg tgcaattcaa aatctgatta gtatagaacc tgatgataaa
     5401 gtatttgctt tcattaacgt taagacattg gaagacgaag agtatatcaa caacaacttt
     5461 attgtgatgt gcacccgtca gggagtgata aagaaaacac cgcttgaagc ttattcgcgt
     5521 gtacgtcaga atggtattaa tgccatagtg attaatgatg gtgatgaatt gattgaagca
     5581 aaacttacca atggtaacaa tgaaatttta cttgctgtaa agtccggtcg tgctattcga
     5641 tttaatgaga acgctgtccg cactgtgggt cgtaactcta ttggtgtaag aggtgtttct
     5701 cttgatcatg ctaaagatga agtgataggc atggtttgca taaaaaacgc aaaagaagaa
     5761 actatcttag tagttagtga aaaaggatat ggaaaacgca gtgacctgga agactatcgt
     5821 gtaacaaacc gcggtggcaa aggtgttaag accatgaaca ttaccgaaaa aacaggtgac
     5881 cttatagcaa ttaagagcgt taaggaagat gatgatctga tgatcatcaa caaatccggc
     5941 ataacaattc gtatggaggt aaaagacctt cgcgttatgg gacgtaacac acagggtgta
     6001 aagcttattc gtttagatga tgatgactcc attgcatcgg tcgcaaaaat tgatatggaa
     6061 attacaggca acggtaatca taaagagaac ggaaacgata cagaagaaaa tcctgttaca
     6121 gacactgaca aacaaaacaa caatcaaaca gaggaaaaca acagtcaacc tgaataatta
     6181 atatttaaac aacaagataa aatgaaaaga attctcacag ctgtgtttat aagtttagcc
     6241 cttggtagtt atgcacagcg cgcacaggta caatcggctt acagctacct taagtacgag
     6301 cagttagaca aagcaaaaga aagcattgat attgcagtaa ccaacgaaaa taccatgaac
     6361 tatgacaaag catggtacta tcgcggactg atttatcagg cactttacaa gaatgaaaaa
     6421 ttcggaaacc tgtcaagcga tccgcttaac gaagccttac gttcatacaa taaagcttta
     6481 gaattaaatc ctaaatttga atatgctgac gatattacag aaaagaaaaa aatattggca
     6541 cagcagtttg ccgacatggg ttatgtaaat tataacttga aaaattttgc aggtgcgctg
     6601 tcagcttatg aaggtgtagt tgctatcatg cccaatgaca cttcatcata cttcaatgct
     6661 gcattgtgcg ctgagcgtgc aggtaataca actaaagcaa aacagttcta caccaaatta
     6721 gatgagatgc aatataaaga tgcaaaaatg tatcacagct atgcgcaact ttatcttacc
     6781 gaaggcgaca caacaaaagc acttgaaata ctatcacgtg gactatcacg cttccctgaa
     6841 gagaaatcaa ttcttattac acagacaaac atttatatca gctctggaaa aacagcagaa
     6901 gcactcaatg ccataaacat ggcaatagaa aaagattcga ccaatgccaa cctttatttt
     6961 gcaaaaggca cattagtaga gaaaactgaa aagagtaaag acgcagctat tgcagcctat
     7021 aaaaaagcta ttgaacttaa agcagattat tttgatgctt attacaatct gggtgcactc
     7081 tattttaacg atgctgcgca tttagccaat gaagccaaca gcatcaagga caataatcaa
     7141 tatgcaaaag ctaaggcagt atttgaagca aagtttaaag aagccaaacc ttatcttgaa
     7201 aaagcctggg agttgaatcc aaaagatcaa agcacaatga tttcattgaa acaactttat
     7261 gctaccatca acgatactgt aaattatgca aaagtgaaag cagcattaga tgcagaaacc
     7321 aaataaaatt attaaatgca taaaaaaagc cctgcaaaac ggggcttttt tgtttattgt
     7381 atcttacttg cattttccta tttcccccgc ttcggttctc tgtcaacttt cactacctta
     7441 gccttaggtt tgtatgccgt ttcatatttc agaatccatt ctttcaccgt catttttttc
     7501 atcaactcgc caatgagttt gtatggaata tcatccatct tcttaaaacg aacacagctt
     7561 tttcccatat ccaatttttg tttgctgtgt tttggaaatt cgcttacaaa ccatttcaac
     7621 agatcaggat cggaatacat acccatgtga taaaaattta ttgattgctt ctgagaagct
     7681 attcctgcaa aaggcaaagg ctcagcaggt ttgcaatggt agccatcagg ataaacagaa
     7741 tgtggtacaa cataacctaa cccaccataa ctgattgcag cctcaaaacc ttttggcagg
     7801 ttttttaata ttacctgatg cagtttttga aatacctcct tcctatcttc ctgtacgttt
     7861 ttcaaaatat catcaacact atttgcaaca tttttcattc taaaagtatt ttttttaata
     7921 tgatttcatc aattacaact tctccacttc aaacaaaatg ctaaagtaaa ccatccttcc
     7981 tactttgggt ccgccataaa tctcataatg cttgttgttg aacagattca ttgcacctaa
     8041 cttaaaagtt gtacctgcat ctttcagatg ataatttact tgtgcatcaa ccatatcgta
     8101 agaaggaatt tctccggtaa attggggtga gccttcatac ttgaaacctt gcacccattt
     8161 atagtttata ttaaaaccaa cacctttaat gtcgcgtccg ttaaaaccta tattgtattt
     8221 attttccggt gtgttgaagg caggaattaa aggatcgtca gtaccttttt tgttcagttt
     8281 attccatgag taatttcctg taaacgcaaa atattttccg atataataat tcaaccccac
     8341 agaaacgccc tgtgtagtta cttcactctg tgcattagaa gccactctca ccacacgatt
     8401 aaaaaccaaa tcataaatag tacccccaaa agtattgata gaaaaagtgt cggcatcaac
     8461 accaatctga tatccgataa aatcatgata ccaggaataa tacccattca aatcaacata
     8521 caaacggtta aacaaagttg cccgataacc ggcttcaatg gttttaacct tttccggtct
     8581 tacaggatca acattgaaga aattcaaaaa tttgaatgac gacttgctac tgtcgtaaaa
     8641 ctcagttacc gattctggag ttaaaagatt ggtgtagcca ttgatgttgc caagcagcgt
     8701 agctctgcct acctgataaa acaaatattg gtcagtaagt gtaggatttc ttacagctga
     8761 tgaaaacgac accctgatga catgattgtt atttggtgta taaacaacag aacctgcagg
     8821 agaaacaagc agattaaaat tctgattttt atccaaacgt accgtagcat tgagttttaa
     8881 tttgtctttc agaattttct tctccaatcc ggcataaaca ccaaattcat aaactgaaag
     8941 tttgttgcgc aaggtgtcta ttgacaatgt atcgctgtta gctgaaatat tataatgcac
     9001 ataactggta tcagaaaaaa ttgttccgtc agaattgggt ttgtaaagac gataatttcc
     9061 tcctactaca ccttcaaccc atttaaatga atagtgatat tcgccttgta tgtgatatag
     9121 tttggactta tcataaaacc gtgaaccccc ttctgaatat gacttgcgtg atgtaattgc
     9181 attaaaagct gaatcgaaac gtgcagttcc gggctcgaag aatgctaagt tttgattttg
     9241 aggattacca acaccactgg cataggcttg tgcctgctga tgataatata ccaatgaatc
     9301 atagtagttg ttcagcaaga aaggattaac agactgaagc cactgcacat aagccggacc
     9361 aaaaccggga ggtggtggaa agcccggaag cttcctgatg tagctcaggt tataattacc
     9421 attccagaaa ttttcatagt catttttcca atggccatca ctcttggcac tacgctgtaa
     9481 caaaagcgct gtaaaataag catcatagga ctgacctgca tcttcggagg tgctgtatgc
     9541 tcttacaaac catttatctt tctctcttac ttcaactctg ttttgtaaaa atttaatgtt
     9601 tttcagactg tagcgattat caccctgata aatggtagtt cctgtaccat aattagaaga
     9661 agcaatgact tctaccattg gtgtaatcat ataatgcaag gcaagacctg ctttggtatt
     9721 gttactgttg taatccacca aatcttcttc tctatatcct tttcggttaa acgacatcag
     9781 tcccggaaat gcacccggaa aatttttata gtctgttcca ttaatatctt catcgccata
     9841 aacatttact gcgtcatagc cacccgggtt atagcttgta ttgcgtgatt gttctgttgg
     9901 cgaatagtta tccgctttcc agtctaatgc agtgaaacga taaaagttga gtttataggc
     9961 aaacttatta tcacctttct tattcttaaa aacctgagca aagcgcaatg caaattcgtt
    10021 gagtttcctg tctcctattt ttaccataaa ctgtaagccc ggtgattcaa aagggctacg
    10081 tgttgtcagg gcgataacac cattaaaagc attaggacca tagtaggcag aacttgctcc
    10141 tacgattaga tcaacttttt gcacatcaag ttcactgctg ccaagaaaat ttcccaacga
    10201 aaaattcagt ccgggtgatt gattatctac accatctatc aactgtaatg aacgcacagg
    10261 cgagctgctg ttaaatcccc ttgtattgat gattttaaat ccaagacttg cactggttaa
    10321 atcaacacct tttaactgac caagaccctc ataaaaaccg gctgcaggtg tttctttaat
    10381 ggcaatataa tcgagtgcct caacagtaat aggtgattcc ttttgttttt ctgaaatacg
    10441 acttccagtt acagtaacat tcttgagcag tacttcattc ttctttagtt ttatggttaa
    10501 tggttgtgcc ccatttaacg ttatctcctg attcaaaaaa cccacaaacg aggctactaa
    10561 aactaaagga taaccacggg gggtttcaaa cgaaaatttc ccttcaatat ctgttgctgc
    10621 gccctctgta gttcctttga ttacgactgt tgcaccaatc aatgtctcac cactgcttcc
    10681 atctttaata acacctgata tcacattttg tgctccggca aaataacctg aaataaaaaa
    10741 caaaaaggct gttaaaaaaa tcctcataaa cttggtttta tttggggtaa aagtaaaata
    10801 cctaaaccga ataaacaata gtacaacatt gcctgactgg ggtattatat atttttgaaa
    10861 atattacatt tgccatccta ttaatgagga taagaagtat ggctaatccg ctgacactgg
    10921 cagagcttga atcaatagca tttaaaagaa aacctgtaaa ggttaatgat gacatcatca
    10981 aatccattaa tgcatcattt gattttttag ttaggttttc aaaagacaaa attatttatg
    11041 gcatcaacac gggttttggc cccatggtgc agtacaaggt tgaaaatcac gaattaaatc
    11101 agttacaata caacctgatt cgtagtcaca gcagtggcac aggtgcagta cttaatgaag
    11161 tatatgcaag ggctgttgtg gcagcacgac ttaataattt tcttcaggca cattcaggag
    11221 tgagcccgga ggtaatttca ctactcacca ccatgctgaa cgaaaatatt gttcctgaaa
    11281 tttatgagca tggtggcgtt ggtgccagtg gtgatttggt tcagttggct catcttggac
    11341 ttaacctgat aggtgaaggt tatgtttatt atgatggtaa gcgccaaaaa acagcagatg
    11401 tttttaagaa attaaacata aaaccattaa cactgcaact gcgtgatggc ttaggcatta
    11461 tcaatggcac atcatgtatg acaggtattg ctgccatcaa ccttgtttat gcaaaacgtc
    11521 ttacgcgttg gatgataact ttgtcggcag tgataaatga aattattgaa tcgtttgatg
    11581 attctttttc tgctgaactc aaccacgtta aactacacaa cggccagcgc gaggtggcac
    11641 aacaaatgcg cgactttttg aaaggaagca aattagtacg taatagaaaa gaactcttct
    11701 ctgatcatgc cgaactgagt cgaacagaat tcaagaataa aattcaggag tattattcta
    11761 ttcgctgtgt accacaaatc ataggcccaa tttatgacac ttgcatggct gctattgctg
    11821 ttactgaaaa tgaattaaat tcaaccaacg acaatccggt ggtcatacct gaagcagaca
    11881 atgtgtttca tggtggcaac tttcatggcg actatatctc tcttgaaatg gataaactta
    11941 aacttgccat ttcaaaactt tcagttcttg ccgagcgaca gttaaacttt ttactgaata
    12001 aaaaactgaa cagaaaattc cctccctttc taaacaagaa aaaattggga ttcaattttg
    12061 gattgcaggg tatgcagttt cttgcaacaa gcactactgc agaaaatcag gcactcagca
    12121 actctgttta tgttcacagc atttcaaaca acaacgacaa tcaggatgtg gtgagcatgg
    12181 gaaccaacag cgccatcatg accagaaaag ttattgataa cagctatgaa gttgttgctg
    12241 tgctcacact agcagtatgt caggcaatag acttattaaa tgttgaagaa tttcaaaaac
    12301 tttgtaaaaa cacactgtca cttcatcaac ttatccgtaa acatgttgca gttgttgaag
    12361 atgatgtgcc acagtttgaa aacatcagaa aagttattga tattttaaaa gaaaatcaga
    12421 tttaaaagat gaattgcgct ttagtaaccg gagcatctag aggacttggc aaagccattg
    12481 cacttcagct tgcagcagat cataagctgc atattctcat caactatgca tcaaacaaac
    12541 aagctgctga agaaacgttg acaataatta aaaatagcgg aggcagtggc gagttattgc
    12601 cgtttaatgt tcaggatcat gaaacagtaa atgcagcgct tgaccgttgg cataaagaga
    12661 atgaagataa atttatcagt gtgctggtta acaatgcagg catcacccgt gatggacttt
    12721 taatgtggat gcccgaaaaa gattgggacg atgttttaaa tatttcactc aaaggatttt
    12781 ataatgttac ccgctacgtt gtgaacccta tgcttcgcaa acgcgcaggc cgaatagtaa
    12841 atattgtttc actttcagga ttgaaaggta atgcaggaca aacaaattat gctgctgcca
    12901 aaggtgctat tgttgccgca acaaaatcgc ttgcttttga agttgctaaa agaaaaatca
    12961 cagtcaacgc tgttgctccc ggttttatca ggagtgatat gactgaagga ctgaacgaaa
    13021 aagaattgag taaattagtt cctatggaac gttttggcac acctgaagaa gttgcgcatg
    13081 ttgtcagctt tctgacatca gacaaagcaa cctatattac cggtgaaatc atcaatgtta
    13141 acggaggtat ttattcctga tttatgcaga gagtcgtcat cacaggtatt ggaatttatt
    13201 catgcattgg aaaaaacatt gcagaagtta cagagtcgtt acgtcaggga aaaagcggaa
    13261 tcatctttga tcagcaaaga aaggatttag gattccgttc tgcacttaca ggctttgtag
    13321 aaacacccga tcttaaagat tttcttcaca gacgtgatcg cttaggtatg catcaaccgg
    13381 caatgtatgc attcatgtca acacgcgaag cacttgctat ggcaatgctc gatgccgatt
    13441 acatggacaa aaatgaaacc ggaataatct tcggcaatga cagcacagca ggatctgttg
    13501 ttgaaactat tgacaaggca agaaaaaaga aagacaacac tctaataggc agtggtgata
    13561 ttttccagaa tatgaactgc acggtgaata tgaatcttgc caccatctac aaacttaaag
    13621 gaataaattt tacactaagt gcagcttgcg caagcgggtc acatgccatt ggaatgggct
    13681 acttactcat aagacaagga ttacaggagc gtgttatctg cggtggagca caagaaataa
    13741 atccacctgc catgaccagc tttgatgggc ttggcacatt cagtagtcgt gaaaacgaac
    13801 cggaaaaagc atcacgtcct ttcgataaaa gtcgtgacgg acttatacct tccggtggtg
    13861 cagcaacggt gatattagaa tcattggagt cggcacaacg cagaggtgcc aacatacttg
    13921 cagaggtaac cggctatgga ttttcatcca atggtgatca tatttcaaat cctgatttta
    13981 acggtcagga acgttcatta aatatggcca tgaaattggc aggaattaat gcatcaaata
    14041 ttgactatgt aaatgcccat gctacctcaa caccagcagg tgatggtatt gaagcaaaag
    14101 ttatttcaca tgttttagga aacaatgttc ctgtaagttc taccaaatca atgacagggc
    14161 atgaatgctg gatggcaggt gccagtgaag ttgtttactc tatgctgatg atgcagaatg
    14221 attttgttgc accaaacatc aattttgaag aacctgatga cgatactaaa accatcaaca
    14281 tcattaccac tgcaaaagaa catcgtatca actgtttttt atccaattca ttcgggtttg
    14341 gcggcacaaa cagtgctttg attgttcagc gtttcaggaa ttgaaattct gtttttaaaa
    14401 gtcatagctg aatacattac aacaattatt tttcacagac tgcataaaac acatttctaa
    14461 gtttacattt gtaacatgag tgttggtgac aaactgcatg agatattaat aaaatactgg
    14521 gggcatagtc gtttcagacc cttgcagcag gaaattattg agtctgtagt tggtggtcac
    14581 gacaccttgg cgcttatgcc aaccggaggc ggcaaatcta tctgttttca ggtgccggga
    14641 atttatctcg atggaatatg ccttgtaatt tctccattga tttcgcttat gaaagatcag
    14701 gtggacaacc tgcgttccag aggaataaaa gcgtctgccg tagtttcggg aatgagcaaa
    14761 acagaaatcg aaattgcctt tgacaattgc atattcggaa agtcgaaatt tctatatctg
    14821 tcacctgaac gtttaacaag cagtcttgca ttaacaaaat tacgagagat aaaaataaat
    14881 cttattgccg ttgatgaagc gcattgtatt tcacaatggg gttacgattt ccgtccctcc
    14941 tatttgcgca ttgttgaact aagagaagca tttccggaag ttcccgtcat tgcactcact
    15001 gcctctgcaa caaaatctgt aagaaatgat attgcagaaa aactcaattt taaaaatcat
    15061 aaattctttc agaaaagttt tgaaagaaaa aacctgaact acatcgttga atacaacaaa
    15121 gacaaactaa cacgcactgc tgagattcta aaatcggtta aaggaagtgc agttatttat
    15181 gttaatagca gaggtaaaac taaagacatt acaacgtatc tgaacagcca aggcattaag
    15241 gcagattttt atcatgcagg actatcagct gcagatagaa acaaaaaaca aagtgactgg
    15301 atgcagaata agatacaagc tatggtggca accaatgcct tcggcatggg gattgacaaa
    15361 gctgatgtgc gaactgttat acatgccgac ttgcctgata gccctgaagc atattatcag
    15421 gaagcaggac gtgcaggacg cgatgaaaaa aatgcttttg cagttttgat ttacaacagt
    15481 gccgacaaag cagacttaga gttgcgtttt caaaatgcat ttcccgaaat aaaacttatc
    15541 agacttatat atcatgccct gggaaattat cttaaagttg ccttaggcag tggagaaaac
    15601 attagtttta attttgatat ttctgatttt gcatcaacct ataacttcaa tcctttagtc
    15661 gctattaatg cattaaaaat aatagcagaa gccggttata ttcatctctc tgatgcagtt
    15721 tatattcctt cacgattaaa atttacagtt gggcaagctg aactttataa ttttcaggtg
    15781 caacatccgg agtctgataa agttattaaa acaatacttc gttcttatgg tggcagcttc
    15841 gataatttta taagaatcag cgaggcagag ttagcaaaaa gattaaacat tgcacaggat
    15901 gtactcatca ggcatttgtt ctttttacaa aagaatgagt tgttagaata cgtgcaacag
    15961 accgacaagc cacagttgac ttatctgatt tcaagggtga aacaggaaac cgtagttcct
    16021 gatttaaagc tattggagtt tcgcaaacaa cgttttaaag aaagagtaaa tgctatgctt
    16081 cactatgcag aaagccttac ctcatgtcgc agtatgatgc tgctgaatta ttttggagaa
    16141 acaacaaaca cacgctgtgg cacctgcgac tattgcagaa aaagaaataa aattgaactc
    16201 aatgacctga cctttgaaaa acaaaatgca gaaataaaag aaattattca gcaacaaact
    16261 ttaagatacg atgagctgct tgaaaaattt cctgcaagta aaaaagaaca atatgttcaa
    16321 tccctgcaat ggctgattga ctcaggtaaa ataattattt cggaaagcga tatacttttg
    16381 tggcatgagt aagttgcatc aatcttttta cttacaggaa agtgctgttg acgttgccaa
    16441 acaattgtta ggcaaagaac tctgtaccaa gataaatggc aaactaacgt cagggattat
    16501 tacagagaca gaagcatata tgggtgttaa cgacaaagca tcgcattcat ggaataacag
    16561 acgcacacat cggacagaaa tcatgtatca tgagggtggt gttgcctatg tttatctttg
    16621 ttatggtatt cattcactat ttaatgttgt cacaaacctt aaagatgttc cacatgctgt
    16681 actgatcaga agcattattc caaaaagagg tattgaaatc atcaaaaaaa gaagacctgt
    16741 caacaagctg atgaaacaaa tcgcatcagg accgggtatg gtttctcagg cattgggcat
    16801 tgactgcagc atgacaggcc tgtctctttt tggagataaa atatggattg aaaatactga
    16861 tctgcaactt tcggcaacac aaattaccgc tactacacgc attggtgttg attatgcagg
    16921 cgctgatgcc ttgctgccct atcgtttttt tataaatcct gaaatatcaa attgatgaaa
    16981 aacctgttta tcagaatcag aaaaatattg tggaagatca ttaaatggtt ttttatttta
    17041 agtattacag gtgtaattct gtttagcttt gttcctgtac catttacccc tttgatgatt
    17101 atccgctttg tggaacaggt ttttgactct gagcgcgaaa tacaattcag gaaagactgg
    17161 gagcctattg caaacatttc gccacttttg gtaaaagcag tgatgaccag cgaagaccag
    17221 cgttttaacg aacactttgg cttagatatg gaggcgattc aaaaagctgt gcagtacaat
    17281 gaaaaacata aaaacaaaaa acggggagcc agcaccattt cgcagcaaac tgcaaaaaat
    17341 gtttttcttt tcccctcacg ctcatggctg cgcaaagggt ttgaagttta ttttactttt
    17401 ctgatagaaa ttttctggag caagcagcgc atcatggaag tgtatttaaa tgtcattgaa
    17461 ttgggcaatg gtatttatgg tgcagaagct gcttcgcaat actactttca taaaccggca
    17521 aaccagctaa gtgtgcatca ggcagcatcg ctggcagcaa ttcttccgct accattaaaa
    17581 tggtcgccca caaaacccaa tgctcgtgtt gtacgaagga ctgaatggat taaacgtaat
    17641 atgaaatatg cagaggggtt agaagagtaa aatttataat atttggacgt ttaacatttt
    17701 agaaatgggt aattgcaact tcgttataag agggtaagat tgctgtgctg tgctttattt
    17761 caatgcttct gatagagtaa ataaaaagaa ttacaaaaat aaacaggtcg gtccataagg
    17821 taaacttttc gctgctacct gaagtctttt ttagtccata ttgaactcct gcaataccat
    17881 aataaattcc actggcaaaa gcacttatta ttctccactt tggtttaaag aaagaaacaa
    17941 acaatatttg cataagtaaa tataagtata tttcctaaat cttatcaaaa aataaaactt
    18001 ttaggaaatc tatcaatgcc aaattttact ccttttttgt cgcattttct tctagttctg
    18061 tttgttgact cgtcctaaaa aagtttacag ttcattttta gacctgtaca ggtttacaat
    18121 agcagagctt tttcggtaat aattcttcaa gaatatttct atgattcttt tttcttttaa
    18181 atgacttttt tctccatgtt ttacacttat tctatgtaaa tttatttcag gacaagtcat
    18241 attataaaat ttctgagaaa aaaagtaccc ggagtggtcc accacaggcg gagaacccac
    18301 acggacgttt ttatattata aaattagtga gaaaaaaagt acccggagtg gtccaccgca
    18361 ggcggagaac ccactttttg ttttcacttg ttgtacccgg agtgggaatc gaacccacac
    18421 ggacgtttaa ggtccacagg attttaagtc ctgcgtgtct accagttcca ccatccggat
    18481 aaaaaggaac ttcaaaaaaa aagttaccga aaaataacag ctaaggttta acacaggcca
    18541 accgttagct acttgtgtgt cggtaacttt tgtcgagagc ggaagacggg gttcgaaccc
    18601 gcgacctcga ccttggcaag gtcgcgctct accaactgag ctacttccgc ataattgctt
    18661 gatatttcaa tacttaaata cccgacttcg ttagaaggga ttgcaaaatt ataaaaaata
    18721 aaataagaac aaatttttta ctttttattt ttcgaaagca gcgttttaat ctcgttaagt
    18781 ttaaagagtg cctctacggg cgtgagggtg ttgacatcgg ttttaagaat ttcgtctctt
    18841 atctgttcta acagcgggtc gtcaagctga aagaagctta gctgcatggc gtctttgtcg
    18901 gcctgatgtc cggcattgcg caacaactct gtgttgctgt gtgccttttc gagcagttga
    18961 agcatttttt cggccttatt aatgacatgt ggcggcatgc ctgccattcg tgccacgtga
    19021 ataccaaaac tgtgttcgct gcctccgggg actaacttgc gcaggaaaag aattttattg
    19081 cccgcttctt ttattgccac attgtagttt tttattctgc tgtaggtatt ggtcatttca
    19141 ttgagctcgt gatagtgtgt ggcaaaaagt gttttagcac gtgcatgttt agattcgtga
    19201 ataaagtcgg caattgacca tgcaatggaa atgccatcat aagtgctagt gccgcgacca
    19261 atttcgtcta acaacaccaa actgcgctgc gtaatgttgt ttagtatgct tgctgtttca
    19321 ttcatctcca ccataaacgt tgattcgcca gaagagatgt tgtcgcttgc ccctactctg
    19381 gtaaagagtt tatctaccca gcctatttct gcactcttgg caggaacaaa actgcctgtt
    19441 tgcgccatga gtactatgag tgccgtttgc ctgagcaagg cagactttcc cgacatgttg
    19501 gggccggtaa tcatgataat ttgctgcgtt tgctgatcta aaaaaatgga gttaggaata
    19561 taggcttcac ctggaggcat ttgtttttcg agtacaggat gtcgtccttc tgtgatggaa
    19621 agaacaaggc tgtcgtttac aacaggacgt gtgtagttgt tgtctaatgc aacattggca
    19681 aacgacaaca ggcagtcgag ctgcgataaa acagatgcat tgacctgtaa tggctgaatg
    19741 tagttttgtg taaagctgat aatctcctga aaaatttcat tctcgatagt tgcaattttt
    19801 tcttcggcac ccagaatttt atcttcatat tctttaagtt ccggagtgat gtagcgctcg
    19861 gcatttacga gcgtttgttt tcttatccaa ctttcgggga ctttattttt atgtgcattg
    19921 gttacttcaa tgtagtaacc aaacacattg ttgaaggcaa ttttgagaga ggaaattccg
    19981 gtttgttcaa cctctcgctg ttgaattttt agcaaataat ctttaccgga aaaagctact
    20041 tgtcttaaat cgtcaagttg ctgattgaat cctttagcaa tgaatccgcc ttttgatgca
    20101 agtggtggag catcttcaac aagtgcatcc tgaatttttt ggatgagcag gttgcaagga
    20161 ttgagctgtt ctgataaaac attaaatgta ctgttgccaa cagcagcaag gttgtctttt
    20221 ataggttgaa tctgagataa agcacgtttg agttgcacca tttcgcgcgg tgaaattctg
    20281 cgcaatgcaa ttttcgaaag catacgttca atatcgccaa tatgacgcag tgatgttgct
    20341 acctgcactc ttaatgattc atctaaggtg aaagccgcaa cggcatcatg ccgctcctga
    20401 atggtttgaa tatcttttaa cggaaggagt atccaccgct tgagtagccg cgcacccatg
    20461 ggtgtgacgg ttctgtctat aacatcaact aaggtgcgtg cattttcatg tggcgaagca
    20521 acaagttcaa gattgcggat ggtaaattta tcgagccaaa catattgttc ttcgtcaata
    20581 cgtgcaatgc ctgtaatatg cgacaatgtt tgttgttgca tttgtccgaa ataatgcaag
    20641 gctacaccac atgccacaat catttcgtcc atttggtgaa taccgaagcc tttaagtgaa
    20701 ttggtctgaa aatgtttgag taaaacttct tcagcatatt ctttctgaaa agcccactga
    20761 tcgagcgtgt aggtgtagaa gccatcgcca aagcgcgcaa caaaatcttt ttgcatggat
    20821 ttctgcaata ccacttcgct gggtttaaaa ttctgaagca gcttttctat gtaggcatct
    20881 ttacctcgtg cagtaaaaaa tgtacctgtt gacacatcag caaaagccac acctgaaaca
    20941 tcttctgtaa gatgaactac tgcaagaaaa ttattgtgtg tgttttcaag aattttttca
    21001 ttgtaggtga caccgggaga tacaagttct gttacccctc ttttgacaat ggtctttgtt
    21061 gtctttgggt cttctaactg atcgcagatg gccacacgct gtccggcacg aaccaattta
    21121 ggcagatagg tgtcgagtgc atgataagga aagcctgcaa gctccatttc tgatgccgca
    21181 ccattggagc gttttgtcaa tactatgccc aaaatttttg aagtctttac ggcatcctca
    21241 ctaaaagttt cgtaaaaatc gcctacacga aagagcagta atgcatcagg atactttgct
    21301 ttgatggcat tgtattgctg catgagcgga gtttcgcttt tatttttttc ttttgtcttc
    21361 aattgtaaaa aatgaaaaag ttaaccggca gcgaaatggg tcgcgcttcg aaagaggggt
    21421 ttaagcaaat acaaaaaatt cctgttgtca ttattcttga aaatatcaga agtgcattaa
    21481 atacaggttc tgttttcagg acctgcgatg cctttccggt agaaaaaatt attctaacgg
    21541 gcatcacggc acaaccacct cacaaagata ttttaaaaac tgcattgggc gctacagagt
    21601 ctgtaaactg gcaatattat tctgatatta aagactgttt tactgaactt aaagcaaagg
    21661 gttataagat ttttgctgca gagcagacaa gccaaagtat tgctatgcct gattttaaac
    21721 ctaatagtga tgaaaagtta gctttagtat ttggtaatga agttgatggt gtaagcaatg
    21781 atacattagc aatgtgtgat ggggcaatag aattgccgca acatggcact aaacattcct
    21841 taaacattgc agtgtgtgca ggcataatga tatatgacct gtttcagaaa atgaaagctg
    21901 cattctaaaa tgaagtttca cacaaaaata gttctttagg agatttgaag tttaatgttt
    21961 tacgtggtct gttgtttaag tcattttcaa cttgtttaat ttgataatgg tttatagtat
    22021 taaagttggt tctcttaggt agatacaccc actggcgcac cccgatagtt atcggggtgc
    22081 gccagtgggg cttaccgatg tggctgccca agagggatat tatgacagcc tcgatgccga
    22141 catgcagtac tgggcgcaaa ggcaggtgta tgagctgctg ctgagcgatg acagccttat
    22201 gctgcaagga accgctgccg acaccctgct tgcgctgtgg aaagacagcg tggctacggg
    22261 caacattggc gcattcagaa ccgttgccga ttcggctata gccaatgccg cttatgctgc
    22321 tgagctgaac tacaacataa ctcctcttaa ccatgccgaa gaaaacgagc aggctgtgta
    22381 tgcgctgtat tttgcattag ccggtgagca gcctgattcg gcacagcatg aaacactgag
    22441 cgccatagct gccgaaaatg ccataagcgg tggtggcgga gtttacatgg cgcgcgccat
    22501 gattgatacc agtgccgatg atgttaccgg aatagcaagc ctgcgaatga ttgcaccttc
    22561 aacaaataag aacataaaca aaacaggcaa attgtatcct aatcctgcta atactgtggt
    22621 gtattatgaa aatgagttag cagcggacg
//
LOCUS       DAOQNP010000049        22571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_777_length_22571_cov_8.728993, whole genome shotgun sequence.
ACCESSION   DAOQNP010000049 DAOQNP010000000
VERSION     DAOQNP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22571
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_777_length_22571_cov_8.728993"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKY09_07925"
     CDS             complement(<1..226)
                     /locus_tag="PKY09_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNB12822.1"
                     /translation="MQATLNASNFNGYNIACFGQQNGTLSVSVTGGTPPYRYEWSNGD
                     SVATLNNLAAGYYFVRVSDSSNQVVETFITI"
     gene            complement(595..1014)
                     /locus_tag="PKY09_07930"
     CDS             complement(595..1014)
                     /locus_tag="PKY09_07930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB12823.1"
                     /translation="MTTKNIQTIQIESKPESIHIIERLVDDLKGEHNIHEDAYGNILV
                     AVTEAVNNAIQHGNKYNPNLKVKISYEVENDTISFAITDQGKGFDYYNLPDPTAPENL
                     EKPTGRGVFLMKHLADQIIFSNNGSSVELFFKTSHVQ"
     gene            complement(1020..4358)
                     /locus_tag="PKY09_07935"
     CDS             complement(1020..4358)
                     /locus_tag="PKY09_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12824.1"
                     /translation="MTGNYSLLIEKLDEFIRKYYRNQVIRGSIYATGITIGFFLFISL
                     AEYFAWMGSSLRTALFFIYVFTVLAVIVRLIIIPLFKTWKLGKTLSYEQAATIIGNHF
                     TEVKDKLLNTLQLNELSENNNNELVQAGIDQKIQNLKPIPFTGAIDFKKNRKYLPLAL
                     IPAGTLIILLFAAPNVIKESTSRLVNYNVAYKKPAPFNFVLLNKDFNVIEQNDFTLNF
                     KVEGQELPDQVYVTYEGRQIKADKEDKQHFTFTFHRITKNTPFQLMADGFYSENYELK
                     SVPNPLISAFKIDLNYPAYLKKKNETLNNTGDLLIPAGTIVKWTFNTVNTSIFKLWFN
                     DSLTPLVSDNNQYICSKKMLASANYSIATANKIITNKDTLKYSINVIPDQYPIIQVEQ
                     QNDSSSFQRIFFKGTVKDDYGFSKLAFQYRFIKSKNDSSSHSDKLNTEILTINKNNNQ
                     TDFYHFWDGALHGVVPGDEVEYFFEVWDNDGVSGPKSAKSQSLIFKAPTLRELAENTE
                     KSNDEIKKDLQESLKQASKIQKQITDAARKLTEKKKTDFEDRKRIEDLLKQQKELDEK
                     LDKIKNEQQQNLAKQNEFKNNESQLAEKQQQIQELFDRLQNPELKELMKQLEQMLSQL
                     DKQSVQEALDKMKLNNKDLEKQMDRTLELFKQLEVEQKLKTTLENLDKLAEDQKKLSE
                     QSMDKNSDAEKLKEQQKDLNKQFEDIKKDIATLEKKNQELEFPQELANTEQEQKDIEQ
                     EQQNSEQQLNENKKSNAGKSQKNAAEKMQNMSQKMKNQMAQSEMEQSEEDMASLRYLL
                     ENLLRFSFEQEALMQSLKTTDVNNPQYVKMGQRQKKLQDDLKIIEDSLFALSKRVIQI
                     QQTVNTEISNIQSNIQKSIAFLQDRQVPVARSSQQYIMTSANNLALLLSEALQQMQMQ
                     QQQQKQGNGSCKKPGKGKPGPSAEKLKMMQQQLSEQMKKMKGKLEGMQKNGGKGDKGM
                     SEELARMAAQQEAIRNELNQLNQQENKDGKNSMGNLGNLANQMEQNERDIVNKKITQE
                     TLKRQQEILTRLLEAENAQREREQDQTRKAEQAKEQFLRNPAAFEEYKKLKEKQTELL
                     NTVPPGLNTFYKNLVNNYFQGLQF"
     gene            complement(4529..5866)
                     /gene="accC"
                     /locus_tag="PKY09_07940"
     CDS             complement(4529..5866)
                     /gene="accC"
                     /locus_tag="PKY09_07940"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNB12825.1"
                     /translation="MFKKILIANRGEIALRIIRTCKEMDIRTVAVYSTADRESLHVRF
                     ADEAVCIGPPPSKDSYLNIPNIIAAAEITNSDAIHPGYGFLSENSKFSAICAKNKIKF
                     IGATPEQIDGMGDKSNAKDTMKKAGVPTIPGSEGLLTSVEEGLKIAAEIKYPVILKAT
                     AGGGGRGMRIVWKDEEFEAAWNSARQEAGAAFGNDGMYLEKYIEEPRHIEIQIAGDQY
                     GKACHLSERDCSVQRRHQKLVEETPSPFMTKELREKMGEAAIKAALAVKYEGVGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPVTEEVINYDLIKEQIKIASGEPISGKNYYPTMHAIE
                     CRINAEDPYNNFRPSPGRITNLHVPGGHGVRVDSHVYAGYTIPANYDSMIAKLITIAQ
                     TREEAINTMERALSEYVIEGVKTTIPFHIQLMKNEDFRKGNYTTKFLESFKLL"
     gene            complement(5941..6435)
                     /gene="accB"
                     /locus_tag="PKY09_07945"
     CDS             complement(5941..6435)
                     /gene="accB"
                     /locus_tag="PKY09_07945"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNB12826.1"
                     /translation="MNKKEIEELIRFVAKAGVSEVHLEQKDFKITIKNPTKEQIVHVA
                     SSVQPVMQMPAPAQPQQMVVAPAAPVATATPAAKPAGDDSKYKTIKSPMIGTFYRTPS
                     PDKPNFVNVGDEVKEGQVICIIEAMKLFNEIESDIKGKIVKVLVEHSTPVEYDQPLFL
                     IDPS"
     gene            complement(6621..11699)
                     /locus_tag="PKY09_07950"
     CDS             complement(6621..11699)
                     /locus_tag="PKY09_07950"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12827.1"
                     /translation="MEKQINSQKLFSALKVVFVFIALFFFKGAIAQTQTFTSSGTFTV
                     PAGVTTVQVEAWGGGGAGGGVASGFLPPNRAGGGGGGGAYLKNTSVTVIPGSTVTITV
                     GAGGTGVNGADGNNGGASSFGGLVTVSGGNGGKHGSNSPANGAAGTGSFGTRNGGNGA
                     AASGGNSGGGGGGAGTTGNGGNASGMTGGTGGAGGGGNGANGRSSNGDGADASALSGG
                     GAGGRNSSSQWGSRTGGDGYRGQVIVSWICPAYNITAISAVTPICPTPGTSDISLTGS
                     VTDLPVGLYTVTYNRSLPSATGLTSTMTVTTAGSGIFTATGLTSSGSSVITVTNLSSG
                     APGGLCSSSITTNNTVAITVNAAPSVTASNDGAVCEGQTIQLHAVATGVTFQWSGPSG
                     FSSTLQDPTVAGAAVANAGTYTVTATDPIAGCSATATTNVVVNALPNANASSNSPVCA
                     GLSINLSTPSVSGASYVWSGPDGFTSTIRNPSISNAQAVNDGNYIVVVTSSAGCTNTS
                     NVTVSVINPPVASASSNSPLCTATTLNLSASPSGATYAWSGPAGYTSSSQNPTRSNAT
                     LAMSGTYTVTVTLGGCSSTSSTTVAVNQAPTGTPTVSNATVCEGSSVTLNANQGLPVS
                     YSASPNVSIPDNNVSGVNSTITIPAQTIASATQLTIGINVNHTWVGDVIAKVTSPSGV
                     ITVFDRPGVPASSNGNSQNLSGYYEFNVSAGTVLPESNSGPTTIPTGTYAPSNTNGTI
                     NTNWSGMTFPMNAGGNWVLNMSDRAGGDLGTLVSWTVTIVTNSGITYTWTSTPNGFNS
                     TDENPTATITGTTTYNLAATANGCTANTTVSVNAIPGPMAMASSNAPICDGNDINFVA
                     DNLAGGQGSGNTYQWYDPNNLPFSSQQNPNIFGATAAYNGIYTVVVTNQYLCTATTSV
                     QVDVNPNPTISISSQINVNCFGESNGSITTNTSNGTGPYAYTIDFINFNVDGIFDNLP
                     TGTYTIYVSDANACQNTVTTTITEPALLQFTKTYTDQQCPSTFGSITMNATGGTTPYM
                     YSYDNGSTFVNNNANLNITAGTYDVMVQDAHMCVTNTEQQNIVLVNTPSTAASTISSQ
                     NGNELCIGGSTILTAVGGTLGSTADWKWYKTNCGATPVGTGPSITVNPSSSTSYYVRA
                     EDVCGATPCADLSLAITVGVPSAAVTVPMTGMPQNVCSGTTANLSINAVGNTTQYIWD
                     APLGSYFNGNPLNVSPFITNTPNVQVTFGNPITSFYKVGIQAANACGSTIRKIHQTRG
                     VVSTPKTILGPGTVCENTNNVAYNIAPIDGATQYQWSITGDATVSGNGTNVNIDFGPT
                     WNNGTVCVAAQTSCFTSPAKCISISKSAGLPNNLSGNFTACPNTNQTYSVTPLPGAAV
                     YNWTLPNGASGSSNNNAINVTFGNGYNSVGDVCVSVTSICGVTSPLKCKTVAPGIPTV
                     PPAITGITNGLCNQSVAYSTPIQSGTSYIWTAPAGANVIGQGNNSVNIQFSSLTTGQV
                     CVVASNTCGASSPRCVAVKGAPNPPTDISALPSSWCANTQGIELTANISNTTGSYTLN
                     WSYPSAPVATYVAGGGNTNNLTLDWGTGNGVISLTASNGCGSATKTYNATTNCRESEE
                     MTATDFTMYPNPASDIVNIEFWSDKDGVQKDLKVLDLSGRTVMVQKIHTIIGRQSVQI
                     SLAQFAKGVYMIELNGQRNKLVIE"
     gene            11955..12722
                     /locus_tag="PKY09_07955"
     CDS             11955..12722
                     /locus_tag="PKY09_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12828.1"
                     /translation="MKQIFWMVLIIIMGVFSVKAQDTKSLIQRTPIKSWYAMNSGEFI
                     FSAGELKISDKAVYDPFGSINTITDPDNKIRFSAFFHTSQELHYNFSNAFGFYTGIAL
                     RNVGFINTMKVGTEKLTIKQRSYSLGIPVALKLGNMQTYFITAGIEPEIMFAYKRKLF
                     FDGDKSKKSQWFSNDVNVFNPSLFAQINFKGGMYIRVKYYLNDFLTNNTESFSLPEKP
                     VSVSYKVDSSKLYYISIGTSIKNRKMLKKPIQTSVGI"
     gene            complement(12756..13616)
                     /locus_tag="PKY09_07960"
     CDS             complement(12756..13616)
                     /locus_tag="PKY09_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNB12829.1"
                     /translation="MITLQNNTLKIRIKKQGAELCSLLNLNNSIEYIWQAKTAWAKHS
                     PVLFPIVGQLKNNEYKWQDKTYKMERHGFARNMLFCVIQNDSHSATFELTDNEITLQQ
                     YPFKFSFQINYSLNGATLIISYKIKNNNAFSMPFSIGAHPAFNIPLSTEETYTDYYLQ
                     FNKKENSGKWELENGLLKNETPFFDNTSEIPLSKSLFENDALVFKNLKSDLIEIKSKK
                     NIYGLSVSTENFPYLGIWAAKNADFVCIEPWHGIADDVNASGNILEKEGIILLNEQQT
                     FTCAFTINVY"
     gene            13795..14181
                     /locus_tag="PKY09_07965"
     CDS             13795..14181
                     /locus_tag="PKY09_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12830.1"
                     /translation="MADAQHCQIKGKVLEKKSMATLDSVKIEIYEGSLMYKIIYSDQG
                     GRFDTKMFQVISNVSFRISRQGYERRVIRFNKLRGNNVIFMDISLRKITAEEESKILR
                     KEEKKHKTKAKRKKYARPGKRILLPF"
     gene            14288..15082
                     /locus_tag="PKY09_07970"
     CDS             14288..15082
                     /locus_tag="PKY09_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNB12831.1"
                     /translation="MGIFLLITSKELMLFSFTAFLLAFIFLIFIFYRSKREAEVQIEK
                     SRLQLQVAQVEMKALRAQVNPHFMFNALNSIYILISHSEMQKAGNYVLKLGTLIRKIL
                     ENSNHQNISLKDDSEVIDLYIQVECMRLNYSFKYQLTINESIDDESVMVPPMIIQPLV
                     ENAIWHGLKNRTEGGLLTINIDSTKDELVFTIKDNGCKADARMTEDEKLRNAKRKSMG
                     MQITKERLDIINQTNNTNARIVEKEIIGDDGSYQGKVVQLYLPLLD"
     gene            complement(15095..15820)
                     /gene="lptB"
                     /locus_tag="PKY09_07975"
     CDS             complement(15095..15820)
                     /gene="lptB"
                     /locus_tag="PKY09_07975"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNB12832.1"
                     /translation="MLLRAENLVKIYKKRTVVNNVSIQVAQGEIVGLLGPNGAGKTTT
                     FYMTVGLIKPNSGKIFLDDNDITALPMYKRAQSGIGYLAQEASVFRKLSVEDNIRAVL
                     EMTKLSQEEQDAKTEELLSEFGLQKIRKSRGDLLSGGERRRTEIARALAVSPKFILLD
                     EPFAGVDPIAVEDIQSIVAKLKEKNIGILITDHNVHETLTITDRAYLLYEGKILKAGT
                     AEDLANDEQVRKVYLGQNFELRN"
     gene            complement(15857..18322)
                     /locus_tag="PKY09_07980"
     CDS             complement(15857..18322)
                     /locus_tag="PKY09_07980"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNB12833.1"
                     /translation="MKTIFQSLAGIALFLLFHSTSLAQLTRISGKVTDALTGEPIPFA
                     SVVFKKSTIGVNTNIDGIYTLESDTPSDSISAQFLGYIAVSLPVKKGQTQTINFLLKA
                     SKVELKEAVIHAGENPANIIIKKVIANRDNNNFEKLNSYSFETYNKLEFDLTNITDKF
                     KNRKIFKPFAFIFDNIDSTTTNEKPFLPIFITESLSDRYLSNNPKDKREIIKASKVSG
                     VENETVTQFLGDMYQNINIYDNFMDVFGKKFVSPISSLGLIYYKYYLLDSLYMGNNHC
                     YKLKFKPRRPEDLTFTGEMYITDTSWAVKKVNMRISNKANINFVEDCAVVKEYEFVDS
                     TMWMLSKDLLVIEFAARDKGLGLIGRKSTSYKNFELNKPIPAQVMKGAQDVVVVEGAL
                     EKDSKFWDAARHDSLNEREKKIYTMVDTIKSLPAYKTYVDVITLFVTGYKTLGKVDLG
                     PYFTVFSFNEIEGYRFRLGGKTNQDFSKHLRLEGFVAYGTKNEQFNYSFGTRYLFTTK
                     PRMGVGFKYRHDVMQLGQSDNAFQDDNILASLFRRSASNKLTYTESEKIYFEKEWLSG
                     VSGRITFEHNEFIPLGKLDYRYYTNENESNIRDKINTSDISAYFRFAYREKFVESKHG
                     RVSLGSKYPVLQAVYTQGLKGVLRSDFDYQKLIVKVDDYLYTAPIGYLYYVLEAGKVW
                     GKLPYPLLEVHRGNESYFYDYLSFNLMNYYEFVSDQYFAFYATHHFEGFFLDKIPLLK
                     KLKWREVASLRGVYGSMSQKNHDLMVDPTYNTLSKKPYLEAGFGVENILKIIRVDFIY
                     RLSYLDNPDISKFGIRGSLQLTF"
     gene            18466..20007
                     /locus_tag="PKY09_07985"
     CDS             18466..20007
                     /locus_tag="PKY09_07985"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNB12834.1"
                     /translation="MKDKLSLLEQKKKEALAGGGTKRIDDQHKKGKLTARERIHFLLD
                     EGSFEEIGMLVTHRATDFGLAKEKYLGDGVVTGYGTIHGRLVYVFSQDFTVFGGSLSE
                     THAEKICRIMDLAMQNGAPVIGLNDSGGARIQEGVVSLGGYADIFYRNTLASGVIPQL
                     SAIMGPCAGGAVYSPAITDFIMMVQNTSYMFVTGPNVVKTVTHEEVSAEELGGASTHS
                     TKSGVTHFSCANEMECIQYLKTLLSYIPSNCEELPPSVEYVSGNENRETLNRIIPDNP
                     NQPYDIREVIAQVADENTFFEVHKDFAENIVVGFARLAGRSIGVVANQPMFLAGVLDN
                     HSSTKAARFVRFCDCFNIPLLVFEDVPGFLPGTDQEWNAIITNGAKLLYAFSEATVPR
                     ITVITRKAYGGAYDVMNSKHIGADMNYAWPSAEIAVMGAKGAAEIIFKKEISKSDNPA
                     DKLKEKEQEYIELFANPYKAAERGYIDEVIEPAQTREKLIKAFKMLENKAAKLPKKKH
                     GNIPL"
     gene            complement(20100..22559)
                     /locus_tag="PKY09_07990"
     CDS             complement(20100..22559)
                     /locus_tag="PKY09_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB12835.1"
                     /translation="MKKQFYNKGKYFLTMLALMISGYTMSQNCSAGFVYSVNPNGVVS
                     FYDSSFVNGNITSATWMFGDGTTGTGTQVTHQYNTSGFITVCYFITTSTGCSDSTCQV
                     ITLNPCTLTATLVYDSLQQNLFAIASGGTPPYSYNWTNGQTLIGIFVPNPGMYCCNIT
                     DSNGCTYTACYLVGGSGCSASFITSIMGNVVVFGNNSQSFSSLLWNFGDGTTSAATSP
                     THTYAQAGTYYACLTAYDGTGAVCSQYCDTIVIVPQTGNTIICGNVFQDINANGFNDN
                     EPGFGNSPVMIWGSGMQYTAMPDSNGNYSISVPPGTYNIVYCVQQPYSLTLPSDSGGC
                     GFYQVTIAAGDTICGFNFGVSLNSSSIVGYVFADSNNNGTKDAGEPGIPYQPVQIGSL
                     TAYTDLTGKYERYVPVGTYTVSYTPSGVYASYPLTTASSNSVNVVGVGITYQGGDYGL
                     NLPAGATNIAVNLLPHTTITPGFPAWYDIQVCNIGINPTGATVTMNYDPGLTYTFGSP
                     VPASVNTSAHVITWNLPTMASGQCLYIQADFSASTSYQIGDATFEFCSAYPTSGTDID
                     LTNNTDTVHQIVTGSWDPNNKLSVQTNNNNPVQQIVSSVNANQEIEYTINFQNLGTAP
                     AVNVVVVDNLSTDLVAGSYQLTGASHNVSVSRNGMNVIYNFQNIMLPDANSNEPMSHG
                     FVSYKINAVNGLVAGTQISDFSKIYFDFNSPVTTNIAVVTMVNPTGINEQGEANLHSV
                     YPNPVSDVATIQFTLGQNSLVGLELFDAAGRSTILMPEVMMQAGLHNTNLNANQLSEG
                     LYLLKLNVNGKSSVMKVTVKH"
BASE COUNT         6664 a         4159 c         4363 g         7385 t
ORIGIN      
        1 taattgtaat aaatgtttca acgacctgat tgctgctgtc ggaaacacga acaaaataat
       61 agcctgctgc cagattgttg agtgtggcaa cactgtcgcc attactccat tcataacggt
      121 agggtggtgt gccacctgtg acactaacac ttaatgttcc gttttgctgg ccaaaacatg
      181 caatgttgta gccattaaaa tttgatgcat tgagcgtggc ctgcatttgt gcatgaatag
      241 tggagctaca aaaaattgct aatgcagcaa gtgcaaaggc tgctgtgtgg ggggggggta
      301 aaagactttt tcattggttg aggattctta attagtgtgt aaatataata cctttttatt
      361 aaaatgcaaa tgattgttgt atggaaatta ggattcagcc ataaaatgaa aaacgctaaa
      421 catgatgatt aaagaaaatg attaacagca aaaaagggtt attaaaaagt aatgacaaag
      481 agtgttgtct tacaatagct gaaaagaaat caagtaaaag taaatgaaac ggaattcaat
      541 atttttgttt cagcagtatg aatgagtaaa ccaataggct atccttttta aaaactattg
      601 cacatggctt gttttgaaaa acagttcaac agacgaaccg ttgtttgaaa aaattatctg
      661 atcggctaaa tgtttcatta aaaagacacc acgtcctgtt ggtttttcaa gattttcagg
      721 tgcagtgggg tcaggtaggt tgtaatagtc aaaacccttt ccctgatcgg taatagcaaa
      781 agagatagtg tcgttttcta cttcgtagct gattttaact ttcaggttcg ggttgtattt
      841 gtttccatgc tgtatggcat tgttaacagc ctctgttacg gccactaaaa tgttgccata
      901 agcatcttca tgaatgttgt gttcgccttt aagatcgtca accaaacgct ctataatatg
      961 tatgctttcc ggtttagatt ctatctgaat tgtctggatg ttttttgttg tcatctgtct
     1021 taaaattgaa gtccttgaaa ataattgttt accaaatttt tgtaaaatgt atttaagccc
     1081 gggggcactg tatttaaaag ctctgtttgt ttttctttaa gttttttata ctcctcaaat
     1141 gcggcagggt tacgtaaaaa ttgctctttt gcttgctctg ccttacgtgt ctgatcttgc
     1201 tctctttcgc gctgtgcatt ttccgcttca agcagtcggg taagtatttc ctgctgcctt
     1261 ttaagtgttt cttgtgttat ttttttgttc acaatatcgc gctcgttttg ctccatttga
     1321 ttggccaaat taccgaggtt gcccatcgag tttttaccat ctttattttc ctgctgattc
     1381 aactggttta actcatttct gatagcctct tgttgcgcag ccattctcgc taactcttcg
     1441 ctcatacctt tgtcgccctt acccccgttt ttttgcattc cttctaactt tcctttcatt
     1501 tttttcattt gttcagaaag ctgttgttgc atcattttta atttttctgc agatggtcct
     1561 ggtttccctt tgcccggttt cttacatgag ccattgcctt gcttctgttg ctgctgctgc
     1621 atttgcattt gttgtaatgc ttcactgagc agtagtgcca gattgttggc cgatgtcata
     1681 atgtattgct gcgagctgcg agccacaggt acctgcctgt cctgcaaaaa tgcaatagat
     1741 ttttgaatat ttgactgaat gttggaaatt tcagtattta ccgtctgctg aatctgaatt
     1801 acacgcttgc taagggcaaa taacgaatct tctataatct tcaggtcgtc ctgcaacttt
     1861 ttctgcctct gacccatttt tacatactgc ggattgttaa catctgttgt tttcaacgac
     1921 tgcatcagcg cttcctgttc aaatgaaaaa cgcagaaggt tttctaacag atacctgagt
     1981 gatgccatat cctcttccga ttgttccatt tcactttggg ccatttggtt tttcatcttc
     2041 tgcgacatgt tctgcatctt ttccgcagca tttttttgcg attttcctgc attcgacttc
     2101 ttattttcat tcaactgctg ctcgctattc tgctgctcct gttctatgtc tttttgttcc
     2161 tgttctgtat tagccaactc ctgtggaaac tccaactcct gatttttctt ttctaaggtt
     2221 gcaatatctt ttttgatgtc ttcaaactgt ttgttaagat ctttctgttg ttcttttaat
     2281 ttttctgcat cggaattttt atccatgctt tgttccgata gttttttctg atcttctgcc
     2341 agcttatcta aattttctaa agtggttttt aacttttgct ctacttctaa ttgcttaaac
     2401 aattccaaag tgcgatccat ttgtttttca agatccttat tattgagttt cattttgtcc
     2461 aaggcttctt gcacactctg tttgtcgagt tgcgaaagca tttgctctaa ttgcttcatc
     2521 agctctttta attcaggatt ctgcaaacgg tcaaacaatt cctgtatttg ctgttgtttt
     2581 tcagctaact gactttcatt gtttttaaac tcattctgct tggcaagatt ttgctgctgc
     2641 tcatttttta tcttgtctaa cttttcgtcc aattcttttt gttgcttcaa caaatcttcg
     2701 atacgcttgc ggtcttcaaa gtcagttttt ttcttttctg taagtttacg agcagcatcg
     2761 gtaatttgtt tttgaatttt tgatgcctgt ttaaggcttt cctgcaagtc tttttttatt
     2821 tcatcattac ttttctctgt attttctgcc agttcccgaa gtgtaggagc tttaaaaatt
     2881 aaagactgtg atttcgcaga cttaggacca ctgacaccat cattgtccca aacctcaaaa
     2941 aaatattcaa cctcgtcacc gggtacaaca ccatgcaatg caccatccca aaaatgataa
     3001 aaatctgttt gattgttgtt cttgttgatt gtaagaattt ctgtatttag tttatcgcta
     3061 tgtgatgaag aatcattttt acttttaata aaacgatact gaaaagccaa ttttgaaaag
     3121 ccataatcat cctttacggt ccctttaaaa aatattcttt gaaatgatga tgaatcattt
     3181 tgttgttcta cctgaatgat tggatactga tcaggaataa cgtttataga atattttaat
     3241 gtgtctttat tagtgattat tttattggcg gtagcaatgg aataattagc acttgctaac
     3301 atttttttgc tgcagatgta ttgattgttg tctgacacta acggtgtcag gctgtcatta
     3361 aaccaaagtt tgaaaatgga ggtattaaca gtgttgaatg tccactttac aattgtgccg
     3421 gcaggtatta ataagtcgcc tgtgttgttt agtgtttcat ttttcttttt aagataggca
     3481 ggatagttta aatcaatttt gaatgcagaa attaatggat tgggtactga ttttagttca
     3541 taattttcag aataaaaacc atcagccatt aattgaaagg gtgtattttt tgtaatgcga
     3601 tgaaaagtaa atgtgaaatg ttgtttatct tctttatcgg ctttaatctg tcttccttcg
     3661 taagtcacat aaacctgatc cggaagttcc tgaccttcta ctttaaagtt aagtgtaaag
     3721 tcattttgtt ctatgacatt aaaatcttta ttaagaagaa caaaattaaa aggtgccggt
     3781 tttttatacg ctacattgta attgacaagt ctgcttgtac tttctttaat aacgtttggt
     3841 gctgcaaaca gcagaataat taaagtgcca gccggtatta aagctaaggg taaatatttt
     3901 ctgttcttct taaaatcaat agcacctgta aatggaatgg gtttcaggtt ttgaattttc
     3961 tgatcaatgc cggcctgaac caattcattg ttgttgtttt cgctgagttc attaagttga
     4021 agcgtattca acagtttatc ttttacttca gtaaagtgat taccgataat tgttgcagct
     4081 tgctcataag aaagtgtttt gcctaatttc catgttttga aaaggggaat aattattaat
     4141 ctgacaatga cagcaagaac tgtaaataca taaataaaga aaagcgctgt tcggagtgaa
     4201 gagcccatcc atgcaaagta ttctgccaat gaaatgaaaa gaaaaaagcc aatagttatt
     4261 cctgtagcat aaatgcttcc tctgattacc tggttgcggt aatacttacg aataaactca
     4321 tcgagttttt caatcagtaa actgtaattt cctgtcatgt ccctctgttc aacatataac
     4381 gctgaagaat ggtttcaggt tatgtgtgcc tttaatttta ataaacattt tacagcctaa
     4441 aagtagtaaa aatgaatagg ccgaatgcca tgcatttacg aatattgcat gaagaggcaa
     4501 ataagtagat attaaaagag agtaagattt aaagcagttt aaacgactcc agaaatttag
     4561 ttgtgtaatt gcctttacgg aagtcttcgt ttttcatcag ctgaatgtga aatgggatag
     4621 ttgtttttac accttctatc acatattcgc ttaatgcacg ctccatagta tttatggctt
     4681 cttctcttgt ttgtgcaatg gtgatgagtt tagcaatcat actgtcgtag ttggctggga
     4741 tagtgtatcc ggcatacaca tgcgagtcaa cgcgcacacc gtggccacca ggtacgtgga
     4801 ggttggtgat tctgccaggc gatggtctga agttgttgta aggatcttct gcattgatac
     4861 gacattcaat agcatgcatg gtagggtagt agtttttacc tgagatcggt tcgcctgatg
     4921 ctatttttat ttgttcttta ataaggtcgt agttgattac ttcttccgtt acaggatgtt
     4981 caacctggat acgggtgttc atttccatga agtagaagtt gcggtgttta tccactaaaa
     5041 attccacagt accaacacct tcgtatttta cagctaaggc agctttaatt gcagcctcac
     5101 ccattttttc acgcaattct tttgtcatga aaggcgaggg tgtttcttca acaagtttct
     5161 gatgtcgtct ttgaacagag caatcacgct cagagaggtg acatgctttt ccatattgat
     5221 cacctgcaat ctgtatttct atatgtcgtg gttcttcaat atatttttca agatacatgc
     5281 catcattacc aaaagctgca cctgcttcct gacgtgcact gttccaagct gcttcaaact
     5341 cttcgtcttt ccacacaatg cgcattcctc ttccaccacc acctgcagtt gcttttaaaa
     5401 tgacaggata ttttatttca gcagcaattt tcaatccttc ttcaacactg gtaagcaatc
     5461 cttctgagcc gggaatggtg ggtacaccgg cttttttcat tgtatccttt gcattgcttt
     5521 tatcgcccat accatcaatc tgttccggag tggcaccaat gaattttatt ttattcttgg
     5581 cacaaatagc gctgaacttt gaattctcag ataaaaatcc atatccggga tgtatggcat
     5641 cagaattggt gatttctgca gctgctataa tgttaggaat attcagatat gaatctttgc
     5701 ttggcggtgg accaatacaa acagcttcat cagcaaagcg aacatgcaga ctttcgcgat
     5761 cggcagtaga ataaactgct accgttctta tatccatttc tttacatgtg cggatgatgc
     5821 gtaaagcaat ttcacctctg tttgcaatta agattttttt aaacataatt agtttggtaa
     5881 tgatgaaatt aagaaagtcc caaggtactt atgattctaa gaaaaatttt gttgtatgaa
     5941 tcaggatggg tcaattaaaa ataatggttg atcgtattct acaggagtgg aatgttctac
     6001 caaaacttta acaattttac ctttaatgtc actttcaatt tcattaaaca gtttcattgc
     6061 ttcaataata catatcacct ggccttcttt tacttcatca ccaacattta caaagtttgg
     6121 cttgtcgggc gatggcgttc tgtaaaaagt gccaatcatt ggcgatttaa ttgttttgta
     6181 ttttgaatca tcacctgcag gttttgcggc aggggttgct gttgcaaccg gtgctgcagg
     6241 agcaacaacc atttgttgag gttgtgcagg agcaggcatt tgcatgacag gctgtacaga
     6301 tgatgccaca tgtacaattt gttctttggt tgggttttta atagtaattt taaaatcctt
     6361 ttgttcaagg tgcacttcac tcactccggc tttggcaaca aagcgaatca gttcttcaat
     6421 ttcttttttg ttcatataca gttgttttta acaagtcttt tcatgaaaat gaatcattta
     6481 tagcataaca actataaaag gtcacttcaa aagacagcat caaaagtaga aactttttac
     6541 aaatatgatg aaatgagcaa aaagaaaaaa gggcagccat ttgggctgcc ctcgctttat
     6601 ttggtttcag cgttgccggt ttactctata accagcttat ttctttgacc atttagttca
     6661 atcatataca ctcctttagc aaattgagct aagctaatct gaactgattg acgtccgata
     6721 atggtatgta ttttttgaac cataacagtg cgtccgctca aatcaagcac tttaagatct
     6781 ttttgcacac catctttatc agaccagaat tctatgttta ctatgtctga cgcaggattt
     6841 ggatacatgg tgaagtcagt tgcagtcatc tcttccgatt cgcgacaatt ggtggttgca
     6901 ttataagttt tggttgcaga accacatcca ttgctggcag taagtgaaat cactccatta
     6961 ccagtacccc agtcaagagt aaggttgttt gtattaccac caccggcaac ataagttgca
     7021 acaggagcag atggatagct ccagttcaag gtataagaac cggttgtatt actgatattg
     7081 gctgtaagtt caattccctg agtgtttgca caccaacttg aaggtaaagc actgatgtca
     7141 gttggcgggt ttggtgcacc ttttacagca acacagcgtg gtgaacttgc tccacaagta
     7201 ttgcttgcaa ctacacaaac ctgtcctgtt gttaatgatg aaaattgaat gtttacagag
     7261 ttgtttcctt gtccgattac attggcaccg gcaggtgctg tccaaatgta gcttgtacct
     7321 gattgtatag gcgtgctgta ggccacagat tgattacaca aaccatttgt aatacctgta
     7381 attgcaggag gcaccgttgg tattccggga gccacagtct tacattttaa tggagatgta
     7441 acaccacaaa tactggtaac gcttacacaa acatcgccaa cactattgta accattacca
     7501 aaggtaacgt ttattgcatt gttgtttgat gatccggagg caccatttgg taatgtccag
     7561 ttataaactg cggcacccgg taatggagtt acagaataag tctgatttgt atttggacat
     7621 gcagtgaaat tacctgagag attattaggt aatccagccg atttactaat gctgatacat
     7681 tttgccggac tggtgaagca ggatgtttgt gcagccacac acacggttcc gttattccat
     7741 gtagggccga agtcaatatt tacattggtt ccatttcctg aaacagtagc atcacctgta
     7801 atactccatt gatattgagt tgcaccatca attggggcaa tattataggc tacattattc
     7861 gtgttttcac ataccgtacc gggaccaaga attgttttag gcgtagaaac cacaccacgt
     7921 gtttgatgta ttttgcgtat ggtacttcca caggcatttg ctgcttgtat accaacttta
     7981 tagaaggatg taatagggtt tccaaaagtt acctgtacat taggagtgtt cgtaatgaag
     8041 ggggaaacat taagtgggtt tccattaaaa taacttccta aaggagcatc ccaaatatat
     8101 tgtgttgtat taccaacagc atttatagaa aggtttgctg ttgttccgga acagacattt
     8161 tgcggcattc cggtcattgg aacggtaacc gctgctgaag gaacgccaac agtaattgct
     8221 aatgataaat ctgcacaagg tgtggcaccg caaacatctt ctgcacgtac ataataagat
     8281 gtacttgaag atggatttac tgtaattgat ggtcctgtac caacaggtgt tgcaccgcaa
     8341 ttggttttat accatttcca gtcagcagtg gaacctaaag taccacctac tgcagtaagt
     8401 attgttgaac caccgatgca aagttcattt ccgttttgag aagaaatagt cgatgcagca
     8461 gttgatggcg tatttaccaa cacaatattc tgttgttctg tattagttac acacatgtgc
     8521 gcatcttgaa ccattacatc ataagtaccg gcagtaatat tcaggtttgc gttgttgtta
     8581 acaaaagtgc taccattatc ataactgtac atgtatggcg ttgttccacc ggttgcattc
     8641 atagtgatgc taccaaaagt ggatggacat tgttgatctg tataagtttt agtaaactgc
     8701 agtaatgccg gctccgttat tgttgtcgtt actgtattct ggcaagcatt tgcatcacta
     8761 acatagattg tatatgtacc agttggaagg ttatcaaaga taccatccac attaaagttt
     8821 ataaagtcaa tggtataggc ataaggaccg gttccattac ttgtgtttgt tgtaatagat
     8881 ccattgcttt cgccaaaaca attaacatta atttgtgaag aaatacttat tgttggattt
     8941 ggattgacat caacctgaac agaagttgtt gcagtacaca aatattgatt ggttacaacc
     9001 acagtgtaga tgccattata agcagcagtt gcaccaaata tatttgggtt ttgctgagag
     9061 ctgaaaggta agttgttggg gtcataccac tggtaagtgt taccactacc ttgaccaccg
     9121 gctaagttgt cagcaacaaa gttaatatca ttaccatcac agataggagc attgctgctt
     9181 gccatcgcca taggccccgg tattgcatta acacttactg ttgtgtttgc tgtacaacca
     9241 tttgctgttg cggcaagatt ataagttgta gttccggtta tggttgcagt tggattttca
     9301 tctgttgaat taaatccgtt tggtgtagat gtccaggtat aagtgattcc agaattagtt
     9361 acaatagtta ctgtccaaga aactaatgtt cctagatcac cacctgctct gtcagacatg
     9421 tttagtaccc agttgcctcc ggcattcatt gggaatgtca ttccactcca atttgtattt
     9481 attgtaccat ttgtatttga aggagcataa gttccggtag gaatcgttgt tggaccagag
     9541 tttgattcag ggagaacagt acctgcactt acattaaact cataatatcc gcttaaattt
     9601 tgactgttac cattacttga agcaggcaca cccggacggt caaatactgt aataactcct
     9661 gaaggtgaag taacttttgc aataacatca cctacccatg tatggttaac atttattcct
     9721 atagtcagct gggtagcaga agcaatagtt tgtgcaggaa ttgtaatagt tgaatttact
     9781 ccgcttacat tattatccgg tatgcttacg tttggtgaag cagaataaga aacaggtaat
     9841 ccctgattgg catttaatgt tacagatgaa ccttcacata cagttgcatt tgaaaccgta
     9901 ggagtacctg ttggtgcctg attgactgca acagttgtag aagatgttga agaacatcca
     9961 cctagagtta cagttacagt ataagtacca ctcatcgcta aagttgcatt gcttcgtgtc
    10021 gggttttgtg aggaacttgt atatcctgca ggcccactcc aagcataagt tgcacctgag
    10081 ggacttgcag aaagattaag tgttgttgca gtacatagcg gggaattgct tgaagcagat
    10141 gcaaccggag gattgattac actaacagtt acatttgatg tattagtaca gccggcagaa
    10201 ctggtaacaa caacaatata gttaccatca ttaactgctt gagcatttga aattgaaggg
    10261 ttacgaattg tactcgtgaa accatcagga ccgctccata catagctggc accagatact
    10321 gatggagttg ataagttaat tgagagtccg gcacaaacag gcgaattgct gctggcattt
    10381 gcattaggca aagcatttac cacaacattg gtggttgcag ttgcagaaca tccggctatt
    10441 ggatctgtag cagttacagt atatgtaccg gcattggcaa cagcagcacc ggctacggtt
    10501 gggtcttgta aggtgctact gaatcctgaa ggacccgacc attgaaatgt aacacctgtt
    10561 gctacagcat gaagttgtat agtttgacct tcacatactg caccatcatt acttgcagtt
    10621 actgaaggcg cagcattaac ggtaatagct acagtattgt tggttgtgat tgaagatgag
    10681 cataaacctc cgggagcacc cgaacttaaa ttggtaacag taattaccga tgaaccactt
    10741 gaagttaaac ctgtggcagt aaaaattcca ctaccggcag tagttactgt cattgtactt
    10801 gttaaaccag ttgcagaagg taaactgcgg ttataggtta cagtatataa tccaacaggt
    10861 aaatctgtaa ctgaccctgt taaactaata tcagaagtac cgggtgtagg acaaatggga
    10921 gtaaccgcag aaattgcagt aatattataa gcaggacaaa tccatgaaac aattacctga
    10981 cctctgtatc catcaccacc tgtacggctt ccccattggc tactactgtt tctgccaccg
    11041 gcaccaccac ctgacaacgc agatgcatca gcaccatctc cattacttga tcttccgtta
    11101 gcaccatttc cgcctccacc ggctccaccg gtaccaccag tcatgccaga agcattacca
    11161 ccatttccgg tagtacctgc tccaccacca ccgccaccgg aattgcctcc ggatgcagct
    11221 gcaccatttc caccattgcg tgttccaaat gaaccggtac ctgctgcgcc attagctggt
    11281 gaattacttc catgtttacc accattacca ccgcttacag ttacaagacc tccaaaggat
    11341 gaggcaccac cattgtttcc atcggcacca ttaacccctg tacctccggc accaactgtg
    11401 atggttactg tactgccggg aattacagta acagatgtgt tttttaaata ggcaccacca
    11461 ccaccgccac caccggcacg atttggaggg agaaagcctg atgctacacc accaccggca
    11521 ccgccaccac cccaggcttc aacctgaacg gtagttactc cggcaggaac tgtaaatgtt
    11581 cctgaggatg taaatgtttg cgtttgtgca attgctcctt taaaaaagaa caaagcgata
    11641 aaaacgaata ccacttttaa tgcactaaat agtttttgcg agttaatttg tttttccatt
    11701 tttagtgatt tttattatga attaaaattt aacccgagca agttaagata gcatcactgc
    11761 tcattaaatt gtaatttggc gaatggtaaa ttttttgggg tgaaaagcaa aaaaataaat
    11821 agcggaacac actatttatc tattttttaa cgcggttatg ccttaaatta aggcacatgt
    11881 ggtttttaaa gtcgtattta aattgatact aaatgcaaat ggaattacta tttttggcct
    11941 aaattaaatt acagatgaaa caaatttttt ggatggttct gattattata atgggtgttt
    12001 ttagtgttaa agcacaagac actaaatcct taattcaacg aacgccaata aaatcatggt
    12061 acgcaatgaa ttcaggagag tttatttttt ctgcaggcga attaaaaatt tctgataagg
    12121 cagtttatga tccatttggt agcataaata caattacaga ccctgataat aaaattaggt
    12181 ttagcgcttt ttttcacaca agtcaggagt tgcattataa tttttctaat gcctttggtt
    12241 tttataccgg tattgctttg cgaaatgttg ggtttatcaa taccatgaaa gtaggtacag
    12301 aaaaattaac cataaaacag cgctcctatt cattaggtat tcctgttgct ttaaaattag
    12361 gaaatatgca aacctatttc attactgcag gtattgagcc tgagataatg tttgcctata
    12421 aacgaaaatt attttttgat ggtgataaat caaaaaaatc acaatggttc agtaatgatg
    12481 taaatgtgtt taacccttct ttgtttgctc agataaattt caaaggtggt atgtacatta
    12541 gggttaaata ttatctcaac gattttctga caaacaatac agaaagtttc agcctgccag
    12601 aaaaacccgt ttctgtcagc tataaagtag attcaagtaa actctattat atttccattg
    12661 gaacaagtat taaaaacaga aaaatgctga aaaaaccaat tcaaacctca gtcggtattt
    12721 gatggttgac aagtgcaaat acacaatgat ttttcttaat aaacattaat agtaaaagca
    12781 caagtaaaag tttgttgttc gtttaaaaga ataatacctt ctttttccaa aatattaccc
    12841 gaagcattta catcatcagc aatgccatgc cagggctcta tgcaaacaaa atctgcattt
    12901 tttgcagccc aaattccgag gtaaggaaaa ttttctgttg aaacacttaa gccataaatg
    12961 ttttttttac tttttatttc aatcaaatct gattttagat ttttaaaaac caacgcatca
    13021 ttttcaaata aactttttga gagtggaatc tcagaggtgt tatcaaaaaa aggtgtttca
    13081 ttctttaaca agccattttc aagttcccat tttccgctat tttctttttt attgaattgc
    13141 aagtagtaat ctgtgtaagt ttcttctgtg cttaaaggaa tattaaaagc aggatgtgcg
    13201 ccaatagaaa agggcataga aaatgcattg ttgtttttaa ttttatagct gataattaac
    13261 gttgcaccat tcaggctata attaatctga aaggaaaatt tgaatggata ctgctgaagt
    13321 gtaatttcat tgtcggttaa ttcaaaggta gcagaatggg aatcattttg aattacgcaa
    13381 aaaagcatat ttcgtgcaaa gccgtgtcgt tccattttgt aagtcttgtc ctgccattta
    13441 tattcattat tcttaagttg accaactatt ggaaataata caggtgaatg ttttgcccac
    13501 gctgtttttg cctgccatat atattcaata gaattattta aatttaacaa actgcataat
    13561 tcagcacctt gcttttttat tctgatttta agggtgttat tttgtaatgt tatcatatca
    13621 aaaaggtgaa atttaaatca atttagtcct caatgataag aaaacattaa ttttagcggc
    13681 aattcaattt tattcagaac attttcagta ttttacgtat aagattaaag tggcttttgt
    13741 caaacaataa tgagtaaatc atttttctgt tttttatttt ttttgtcagt acaaatggca
    13801 gatgcgcagc attgtcagat aaaaggtaag gttttagaga agaaaagtat ggcaacactc
    13861 gattctgtga agatagaaat ttacgagggg agtcttatgt ataagataat ttattcagat
    13921 caaggtgggc gatttgatac taaaatgttt caggtaatca gcaatgtaag tttcaggata
    13981 tctagacaag gatatgaaag acgagtcatc aggtttaata aactgagggg taataatgtc
    14041 atttttatgg atatttctct cagaaaaatt actgctgagg aagagtctaa aattctgcgc
    14101 aaggaagaga agaagcacaa aactaaggca aaacggaaaa aatatgccag accgggaaaa
    14161 cgaattttgt tgccatttta aattttaaag aaacatcaca tttttcaggg atttaagtta
    14221 ttttagccaa tagattttct gtgcttaagg ttagtgaagt attttttaat ccgggttttg
    14281 tattcttatg ggaatttttc tgcttattac ctccaaggag ctgatgctgt ttagttttac
    14341 agcgttttta ttggcattta tttttttgat ttttattttc tatcggtcaa aacgagaagc
    14401 agaagttcag atagaaaaaa gccgcttaca gttgcaggtt gcacaagtag aaatgaaagc
    14461 actgcgtgca caggtgaacc cacattttat gttcaatgca ttaaacagca tctacatttt
    14521 aattagccac agtgaaatgc aaaaagcagg taactatgtt ttaaaactag gtacattaat
    14581 ccgcaagatt ctcgaaaact caaaccatca gaatatttct ttgaaggacg atagtgaagt
    14641 tattgatctt tatattcagg tagaatgtat gaggcttaat tatagtttta aatatcagtt
    14701 gacaataaat gaaagcatag atgatgaaag tgtcatggtt ccgccaatga ttattcaacc
    14761 gcttgtggaa aatgcaattt ggcatggatt aaaaaacaga acggaaggtg gtcttcttac
    14821 catcaatatt gacagtacaa aggacgaatt agtttttaca ataaaggaca atggttgtaa
    14881 agcagatgcc cgaatgacag aggatgaaaa actacgtaat gccaaacgaa aatcaatggg
    14941 aatgcaaatc acaaaagagc gtttggatat tatcaatcaa accaacaata ccaatgccag
    15001 gatagttgaa aaagaaatta ttggtgatga tggaagttat cagggtaagg ttgtgcagct
    15061 ttatcttcct cttttggatt aatactcttg cttcttaatt tctgagttca aaattctgac
    15121 ccagatatac ttttcgtacc tgctcatcat tagcaaggtc ttcagcagta ccggctttta
    15181 aaattttacc ttcatataac agataagcac ggtcagttat tgttagcgtt tcgtgaacgt
    15241 tgtgatcagt aattaaaatt cctatatttt tttctttaag ctttgctaca attgattgta
    15301 tgtcttcaac ggcaatgggg tcaacacctg caaatggttc atcgagcaga ataaattttg
    15361 gacttacagc taaagcacgc gctatttctg ttcgtctgcg ttcgccaccg gatagtaaat
    15421 caccacggct ttttcttatt ttctgtaatc cgaattcact aagtagttct tctgtcttgg
    15481 catcttgttc ttcctgtgaa agttttgtca tctccaatac agctctaata ttatcttcaa
    15541 cacttagttt acgaaaaaca gaagcttcct gagctaagta tccaataccg gactgcgccc
    15601 ttttgtacat tggcaaggcg gtaatatcat tatcatcaag aaaaatttta ccgctattgg
    15661 gttttatcaa tccaaccgtc atataaaaag ttgtggtttt accggcacca tttgggccaa
    15721 gtaatcctac tatttcacct tgtgctacct gaatagacac attgtttaca acggttctct
    15781 ttttataaat ctttactaag ttttctgcgc gtaacagcat ttttgtctat tcaatatggt
    15841 gcaaagataa cagctatcag aatgttaatt gcaaacttcc ccggattccg aattttgaaa
    15901 tatcaggatt atctaaataa ctgagacgat aaataaaatc aacacgaatt atttttaata
    15961 tattttctac tccaaagcct gcttcaagat agggtttttt agacagtgta ttgtaagttg
    16021 ggtcaaccat caaatcatga tttttttgtg acatgctgcc ataaacaccg cgaaggcttg
    16081 ccacttcgcg ccactttaat tttttaagta atggaatttt atctaagaag aaaccttcga
    16141 agtgatgtgt agcatagaat gcaaaatatt gatcgctgac aaattcatag taattcatca
    16201 ggttaaaaga aagataatca taaaaatagg actcattgcc acgatgcact tccaataagg
    16261 gatagggtaa cttaccccaa acttttccgg cctctaaaac atagtataaa taaccgatgg
    16321 gtgcagtgta taaataatca tctactttaa caatcagctt ctgatagtcg aagtcgcttc
    16381 gtaaaacacc ttttaaacct tgtgtatata cagcctgtag caccggatat ttactaccca
    16441 aacttactct gccatgtttg ctctccacaa atttttcacg ataggcaaac ctgaaataag
    16501 cagaaatatc agaagtgttt attttatctc tgatgttgct ttcgttttca tttgtatagt
    16561 aacgataatc taatttccct aaagggataa attcattgtg ttcaaaagtg atccgtccac
    16621 taactcccga aagccattct ttttcaaaat aaatcttttc actttctgtg taggttaatt
    16681 tgtttgatgc tgatcttctg aaaagcgaag caagaatatt atcatcctga aaagcattat
    16741 cactttgacc gagttgcatt acatcatgtc tgtatttgaa tccgacaccc atgcgaggtt
    16801 ttgttgtaaa taaatagcgt gttccaaagc tgtaattgaa ttgttcgttt tttgtaccat
    16861 aagcaacaaa tccttcaagt cttagatgtt tactgaaatc ctgattggtt tttccgccaa
    16921 gacgaaaacg atatccttca atttcattaa agctaaatac agtgaaatac ggacccaaat
    16981 caactttgcc taatgtttta tatcctgtaa caaaaagtgt aattacatca acataagttt
    17041 tgtaggctgg caacgattta atggtgtcaa ccatagtgta aattttcttt tcgcgttcgt
    17101 tcaatgagtc gtgtcgtgct gcatcccaaa atttgctgtc tttctctaat gccccttcaa
    17161 ccacaacaac atcctgtgcg cctttcatta cctgtgcagg tattggttta ttcaactcaa
    17221 agtttttata agaagttgac ttgcgcccaa tgagtccaag tcctttatcg cgtgcagcaa
    17281 attcaataac cagcaaatct ttggagagca tccacatagt actgtccaca aattcatatt
    17341 cttttacaac ggcacagtct tcgacaaagt ttatattcgc tttattggaa atacgcatat
    17401 ttactttttt aactgcccat gaagtatctg taatatacat ctctcctgta aaagtaagat
    17461 cttcgggtct gcgcggctta aatttcaact tgtagcaatg attgttgccc atgtacaaac
    17521 tgtcgagcag ataatatttg tagtaaatta atccaagact cgatataggg ctgacaaatt
    17581 ttttaccaaa cacatccata aaattatcat agatatttat gttttgatac atatcaccca
    17641 aaaactgcgt tactgtttcg ttctcaacac cggatacctt acttgcttta atgatttcgc
    17701 gcttgtcttt aggattgtta gagagatagc ggtcgcttaa cgattctgtt atgaaaatag
    17761 gaagaaaagg tttttcattg gtagtggtgc tgtcaatatt atcaaaaatg aatgcgaagg
    17821 gtttaaaaat tttacgattt ttaaatttgt cggtaatgtt agttaaatca aactctaatt
    17881 tattgtatgt ttcgaaggag taactgttta gcttttcaaa attattgtta tctctgtttg
    17941 caataacttt tttaatgatg atattggccg ggttttctcc tgcatgaatc actgcttctt
    18001 tcagctcaac cttgcttgct ttaagtaaaa aatttatagt ttgtgtctga ccctttttta
    18061 ccggtaggct tacagctata taacccaaaa actgagcaga gatgctgtca cttggtgtgt
    18121 ctgactctaa agtgtagatt ccatcaatat tggtgttgac acctatggtg gattttttaa
    18181 aaactacgct ggcaaaaggt attggctcac cggtgagtgc atcagtaact ttacctgaaa
    18241 ttctggtaag ctgtgccaga gaagtactgt gaaaaagaag gaataaagca atgcctgcaa
    18301 gcgattggaa aatagttttc acaaattgat ttatgtagaa gtttttacct tgctaagaac
    18361 ggaaaaaagt tcgattcctt gcttaacaag gttttagttt tattttaaac gggtagttct
    18421 tcatttagtt ttttaagttt gcacaataga ttaatcaaca aagcagtgaa agataaatta
    18481 tcattacttg aacagaaaaa gaaggaagca ttagccggtg gcggtaccaa gcggattgac
    18541 gatcagcata agaaaggaaa actaacggca agagagcgga tacatttttt acttgatgaa
    18601 ggcagttttg aggagatagg tatgctggtg acacatcgtg caacagactt tggtttagca
    18661 aaagaaaaat atcttggtga tggtgttgta accggttacg gaacaattca tggcaggtta
    18721 gtgtatgttt tttcgcagga ttttactgta tttggtggct cactgagtga aacacatgca
    18781 gagaaaattt gtcgtataat ggatttggca atgcaaaatg gcgctccggt aatcggttta
    18841 aatgatagcg gaggtgcgcg cattcaggaa ggtgttgtat cattgggggg ctatgcagat
    18901 attttttatc gcaatacgct agcttcagga gttattcctc aattgagtgc tattatgggt
    18961 ccttgtgccg gtggtgcagt ttattcacct gccataacag attttattat gatggtacaa
    19021 aatacgtctt atatgtttgt taccggccct aatgttgtaa aaactgtgac ccatgaggag
    19081 gtaagtgcag aagagttggg tggagcatca actcattcaa ccaaatcagg tgtaacacat
    19141 ttttcatgtg ccaatgagat ggaatgcatt caatatctca aaactttact gagttatatt
    19201 ccgtcaaatt gcgaggagct gcctccttct gttgaatatg ttagcggtaa tgaaaaccgt
    19261 gaaacattga accgtattat tcccgataat cccaatcagc catacgacat tcgtgaagta
    19321 attgcacagg tagcagatga aaacactttt tttgaagtac ataaagactt tgcagaaaat
    19381 attgttgtgg gctttgcaag gttagccggt cgcagcattg gcgtagttgc taaccaacca
    19441 atgtttctgg ctggtgtttt agataatcat tcatctacta aggcggcacg gtttgtacgc
    19501 ttttgtgatt gctttaatat tccgttgctg gtatttgaag atgttcccgg atttttaccc
    19561 ggaacagatc aggagtggaa tgcaattatt accaatggtg caaagttgct ctatgcattt
    19621 agtgaagcta ccgtaccacg cattaccgta ataacacgca aagcgtatgg tggtgcttat
    19681 gatgtgatga actccaaaca tattggtgcc gacatgaact atgcatggcc ttcggcagag
    19741 attgcagtta tgggagcgaa gggagcagca gaaattattt tcaaaaaaga aatcagtaag
    19801 tcagataatc ctgctgataa acttaaggaa aaagaacagg aatacattga gttatttgcc
    19861 aacccttata aagcagcaga gcgtggttat attgatgaag ttattgagcc tgcacagaca
    19921 agagaaaaat tgattaaggc atttaaaatg cttgagaaca aagcagctaa attgcctaag
    19981 aagaagcatg gaaatattcc tttgtagaga ggaatatatt ttattacgta tagtataata
    20041 ttaaaacaag aagccctcct tttaagaggg cttctttaat gattgactaa aaatcaaggt
    20101 caatgtttaa ccgtaacctt cattacagat gattttccgt tgacattcaa cttaagaaga
    20161 tacaatcctt ccgaaagctg attggcattt aaatttgtgt tatgtaaacc tgcctgcatc
    20221 atgacttcgg gcattaatat ggtgctgcgt ccggctgcat caaacaattc aagtccgact
    20281 aaagaatttt gtcctaatgt aaactgtatg gttgcaacat cactgacagg attaggataa
    20341 acactatgta aattggcttc accttgttcg ttaattcctg tcggattaac catggtaaca
    20401 acagcaatat tggtagttac cggactatta aagtcgaaat aaatttttga aaaatcagaa
    20461 atttgtgttc cagctacaag tccgttaaca gcattgattt tatatgacac aaaaccatgg
    20521 ctcatcggtt cattgctgtt ggcatcagga agcataatgt tctgaaaatt ataaatcaca
    20581 ttcatgccat tgcgacttac agatacattg tggcttgcac ctgttaactg ataagaacca
    20641 gcaaccaaat cagtagagag gttgtcaaca accactacat tcactgcagg tgctgttcct
    20701 aaattctgga agttaatagt atattcaatt tcctgattgg catttacaga agaaacaatt
    20761 tgttgaacag gattgttgtt gttggtttga acagaaagtt tattgttcgg atcccaagaa
    20821 ccggtaacaa tctgatgaac tgtatcggta ttgttagtta agtctatatc agttcctgat
    20881 gttggatagg cactgcagaa ttcaaacgtt gcgtcaccaa tctgatagga tgttgaagca
    20941 ctgaagtcag cctgaatata aaggcattgt ccgcttgcca ttgttggaag attccatgta
    21001 atgacatgag cagaggtgtt tacagatgcc ggaacaggac taccaaaggt gtaggttaag
    21061 cccggatcgt agttcatggt aacagtagca cctgttgggt tgatgccaat gttgcaaacc
    21121 tgaatatcgt accatgcagg aaatcccggg gtaatagttg tatgaggtaa taaattgact
    21181 gcaatgtttg ttgcacctgc aggtaaatta agcccataat caccaccctg ataagttata
    21241 ccaacgccta cgacatttac gctgttgcta ctggcagtgg ttaacggata agaggcataa
    21301 actcctgagg gtgtatagga cacagtataa gttccaaccg gtacataacg ttcatatttt
    21361 cctgttaaat cagtgtaggc agtaagtgat ccaatttgta caggctgata agggataccc
    21421 ggctcaccgg catctttagt tccattatta ttactgtctg caaaaacata acctactatt
    21481 gaagaactgt tgagcgaaac gccaaagtta aagccgcata tagtatcacc tgctgcaata
    21541 gttacctgat aaaaaccaca gccaccggaa tcagacggta aggtaagact gtaaggttgt
    21601 tgaacacagt aaacgatatt ataagttccc ggaggaacac ttatactata gttaccattt
    21661 gaatcaggca tagcagtata ttgcattcct gaaccccata tcattaccgg tgaattccca
    21721 aagcccggtt cattatcatt aaaaccattg gcattaatat cctgaaaaac attaccgcaa
    21781 ataatagtgt ttccagtttg tggaacaata acaattgtat cacaatattg tgaacacact
    21841 gcacctgttc catcgtaagc agttaaacat gcataatacg ttcctgcttg tgcataagta
    21901 tgtgttggtg atgtggcagc agatgttgtg ccatcgccaa agttccacag cagtgacgaa
    21961 aaactttgtg agttattacc gaagacaact acattgccca tgatgcttgt aataaaggaa
    22021 gcgctgcatc cactgccgcc taccagatag caggctgtgt aggtgcagcc attggaatca
    22081 gtaatgttgc aacaatacat acccggattg ggaacaaaaa tacctattaa agtttgacca
    22141 tttgtccaat tatagctata cggaggagta cctcctgatg caatagcaaa tagattttgc
    22201 tgtaatgagt cataaacaag tgttgctgta agtgtacatg gatttaacgt gatgacctgg
    22261 caagttgaat cactgcatcc tgttgaagtg gtgatgaagt agcaaaccgt aatgaaacct
    22321 gaagtattgt attgatgggt tacttgggtt cctgtgcctg tagtaccatc gccaaacatc
    22381 caggtggcac ttgtaatgtt gccgtttaca aagctagagt catagaatga gactactccg
    22441 ttggggttga cactataaac aaaccctgca ctgcagtttt gtgacattgt atagcctgat
    22501 atcatcagag ccagcatggt aaggaagtat tttcctttgt tgtagaattg ttttttcatg
    22561 ttattgaatt t
//
LOCUS       DAOQNP010000050        22545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_778_length_22545_cov_9.165362, whole genome shotgun sequence.
ACCESSION   DAOQNP010000050 DAOQNP010000000
VERSION     DAOQNP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22545
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_778_length_22545_cov_9.165362"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKY09_07995"
     CDS             <1..847
                     /locus_tag="PKY09_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNB12836.1"
                     /translation="IAQMKKGATLISFMWAATNADLVEACKQAGINAFSMDAIPRISR
                     AQKMDALSSQANLAGYKAVILCADTLGKILPMMTTAAGTIRPSKVVIFGAGVAGLQAI
                     ATAKRLGAVVEVSDIRPETKEQVQSLGGKFIEVKGDDSIKIEGGYVKGVSEEFLKRQQ
                     ELVAKHIKEADIVITTALIPGKKAPLLVTEEMVKSMKNGSVILDMAVEQGGNVTGSEL
                     NQTVQKNGVTIIGESNIPSLLPMNASDLYAKNIQTFLLHLTTKDGFKWEMEEEITKGS
                     LIVKS"
     gene            873..1178
                     /locus_tag="PKY09_08000"
     CDS             873..1178
                     /locus_tag="PKY09_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNB12837.1"
                     /translation="MTEIINFIGTHQEIIFFIILSVFVGIEVIGGVPTVLHTPLMSGA
                     NAIHGVVIVGAIIVMLNVDANDYVALSLGFLAVVLGTLNVVGGFVVTDRMLEMFKKK"
     gene            1210..>2489
                     /locus_tag="PKY09_08005"
     CDS             1210..>2489
                     /locus_tag="PKY09_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNB12838.1"
                     /translation="MTPEVLYNIRTITYIIASATFIIGLKRMGNAKTARNGNLIAAVG
                     MTLAIIASIFIHVNKVQTETGVAFEQVETPGFIYSLIFAAIIIGTIIGWLTAKKVQMT
                     KMPELVSMFNGMGGACAALIGLMEFHHNTGNTGALIAIVLGLIIGSVSFSGSVIAFLK
                     LNGTMNKPVRLPSYNIINTLVMIGVFGFGAYMIFSGTESSTLLYLLFAAALIYGILFT
                     IPIGGADMPVVISLLNSFTGLAAAFGGFLYDNKVMLTGGILVGSAGTLLTLVMCKAMN
                     RPLSNVIFGAFGGGGASAGDGPDVKGSIKDMSVADAAVLLNYSKKVVIVPGYGLAVAQ
                     AQHIIHELEMLLEERGVEVKYAIHPVAGRMPGHMNVLLAESNVDYGKLVEMEEINPEL
                     EQTDVVLVVGANDVVNPAAKTDPSSPIYGMPILEV"
     assembly_gap    2490..2589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2590..4681)
                     /locus_tag="PKY09_08010"
     CDS             complement(<2590..4681)
                     /locus_tag="PKY09_08010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNB12839.1"
                     /translation="MPFNLNLKKINIRYILSFLLFLSYFTSFANTDTLTFFGSKPAKQ
                     DTIYSLNVINNFKWRVKVYSKEGIVNSFTTTSIDTFMFRNHEPEGLLLSTTFLVDSNM
                     LNKVKYLFYSTNGSVKVLLNNTTITSDGIFNNSTKYTDLQSLKENYISFVLTQPKNNL
                     QVLYLPNQNSKIFSLHLRLGEEKWAKENQLENQRDEVRHYAVATFYFSFAVVLILFYF
                     FIRNKEYLFFGLYCLATAIQYLTNVLTQSPVVGVISTYSFLISLELLAIFMALAINNI
                     NKTKTPLLIMCLVMLIALLIPNDIIFTINFVRNIEVPVITLLSSLIFFSYSIFSFAYH
                     WVQGFGQKKWDVKVLTYGTALGLILLILPIIYTASKNMYQQNTSSDILTLFFTISPLI
                     YPIMVAIVLARRYGQNQQQLIEQVVTIKKLGEENLLKETEKKNILAQQNILLENKVTE
                     RTFELEEKNKEILSKNKEITDSLIYAKRIQAAVLPNLNTVYKILDQSFILYIPKDIVS
                     GDFYEFTQKNNKHIIAAADCTGHGVAGAFMSIIGSSLIKSIINEKNITTPSGILDALN
                     EGIIHSLKQHESETHDGMDISICSLDIEKMSLEFAGANRPLWLIRNSELIVYAADKFP
                     IGGLQIKHNNQFTNHQIKIQQKDTIYLFSDGYADQFGGEQGKKLMTKKLKEILVSIQH
                     LGMSEQKVYLQNYFN"
     gene            4794..6173
                     /locus_tag="PKY09_08015"
     CDS             4794..6173
                     /locus_tag="PKY09_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNB12840.1"
                     /translation="MITVIIPTLNEEENVANVIKFAKQYSLVTEVLVVDDKSIDNTVR
                     IAQDSGARVITSTRIGKGGSMREGVMFATNEVVVFLDADINPYPHFTIKLLTEPILQG
                     GADFVKSSFNRNAGRVTELVAKPMLSIFFPELLQFQQPLSGMIASKKSLLQKLDFRED
                     YGVDIGLLIDMHLIHANIQEVDIGYIENKSKPWQALGKMSKEVAQTIILKASSSNSPH
                     YNFEEFGVLNEIRSQMDFALASQQESLSKLIVFDMDNTILRGRFIDTCAEKFNFKTQL
                     MDIRSSETDSTILTKRIAKLLKNRTIDELIDVADSIDIVADSEKVIDNLKTQGYVVGI
                     ISDSYDCITNHVKNKLRMDFSLSNELEFSKSICTGEVKIPSFFVNNENSSCKHVLCKT
                     NALHKLLEKYNIKKENSVAIGDSMNDLCMIKEAGLGIAFCSKDELLNHHADVIIRKPE
                     FSELLNLTK"
     gene            complement(6233..6835)
                     /locus_tag="PKY09_08020"
     CDS             complement(6233..6835)
                     /locus_tag="PKY09_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12841.1"
                     /translation="MRTILSSMLLLISTMLFAQEKPELGNNIFSITPMTIISNEPMDN
                     NTDVAIGINYERIVSNGLIGIQLPVNLSLRNPGFFYFMPTIKLYPTKQGVVRYAVGPQ
                     FYFASGQVEHSRTESVWNGNQYIYTRYKHSDNHTQLGFMINNSLNVTISKTLYLGMDL
                     GIGINYFDSASEDEETNNDSFDTTPISPSVKFSFAMGYRF"
     gene            6973..9399
                     /locus_tag="PKY09_08025"
     CDS             6973..9399
                     /locus_tag="PKY09_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNB12842.1"
                     /translation="MADQTVVTENKELSFEEFRQRVLNDYRLGRISRETSLLGRREVL
                     TGKAKFGIFGDGKEVAQIAMAKVFQNGDFRSGYYRDQTFMMAAGLLTVQEFFAQLYAH
                     PSVAAEPATAGRAMNGHYATRLLNDDGSWKNQTQSKNSSADISPTAGQMPRLLGLALA
                     SKFYRNNPELKSDKFKNFSVDGNEVAFGTIGDASTSEGLFFETINAAGVLQVPMVISI
                     WDDGYGISVPNKYQTTKQSISEALQGFARTQEKPGYEIIKAKGWDYAELCDAYEQAAK
                     IARTEHVPVMVHIQQMTQPQGHSTSGSHERYKSKERLMWEEEFDCLRKMREFMVQSAI
                     ATEQDLDTIDTEAKKFVNDGKKQAWDSFTTEINAERQQALSLAEAAISRSSNGVFLNK
                     AVEEIKSITNCERRDIAVFVQQVLFTLRGENSAELETLKQWYRQWHQLNFDRYSSQLY
                     AEGIGSALGIAEVKAVYSAEANMVDGREVLLANHDILFSRYPEYIAFGEDVGTIGGVN
                     QTFAGMQAKYGEHRVFDVGIREATIAGQGIGMAVRGLRPLAEIQYLDYLAYAIQILSD
                     DLACLRYRTKAGQKAPLIVSTRGHRLEGIWHSGSPMQFILGALRGMLVLVPRNMTQAA
                     GFYNLLLQCDDPALVIEPLNGYRLKEKLPQNLGEFTVPVGVPEILEEGNDVTLVTYGS
                     CVRVAQEAMKQLQLCGISVELIDVQSLIPFDIHHIIAESIQKTNKVVFFDEDVPGGAT
                     AYMMQQVIENQKAFEFLETPPVTLTAKEHRPAYGSDGDYFSKPNAEDVFNTVYELMHA
                     VNPQAFPLFR"
     gene            complement(9420..9986)
                     /gene="ruvC"
                     /locus_tag="PKY09_08030"
     CDS             complement(9420..9986)
                     /gene="ruvC"
                     /locus_tag="PKY09_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNB12843.1"
                     /translation="MSESEKIILGIDPGTNVMGYGVLVISGTEMKLETAGIIHLEKFD
                     NHPLKLKHIFEKTLRLIESYLPDEMAIEAPFFGKNVQSMLKLGRAQGVALAAALFKNI
                     PVFEYSPKKIKQSVTGNGSATKEQVAAMLQQLLHFEEMPKYLDATDGLAAAVCHHFQK
                     NSGQSQLSGSKSKGWQKFISENPDKLIG"
     gene            10067..11011
                     /locus_tag="PKY09_08035"
     CDS             10067..11011
                     /locus_tag="PKY09_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNB12844.1"
                     /translation="MLQFLLKAAIALFSLWFIVHKVIHRENEMSFFGFVNTRIHTEGV
                     YSILISVIILMVLNWSIEAIKWKFLINKLTKLSFVRSLSAVFAGATVSFFTPNRVGDY
                     AGRMLFLPVSVRIASLLSTFVGNISQLIVTLCCGLAAMALSLQHYVQWNESLVVTTRV
                     ASFVFALILFISFPYIRVVFKMPLLNFVRQRFPEYVMHLENYSNKEMMQILFLSLLRY
                     SVFAFQYMLLFLVSGIPVVPSMMVIVALIFFIQALVPTLALTEFAIRGSVALMVVAPF
                     IQNDEAVLMASFALWLINLAVPAAIGSLMIFYFNFRKE"
     gene            complement(11018..11890)
                     /locus_tag="PKY09_08040"
     CDS             complement(11018..11890)
                     /locus_tag="PKY09_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12845.1"
                     /translation="MRFIISFVILFTVHYSGYSQERRGPFVKTDLFTLDRDRVIDGGI
                     FSYRIAVDADEIIAFNSCNQPYVFLINNRGTILEEIKLPYEGCLRNMEFDEFDNLMMM
                     DNEETRIFKYVRKTKKIEIIAYNKPEDWYKSLNHFYRFFEIASIPTYYNNPDYYQDFY
                     KTRFHYSYNLWLNYQDGYIYQFAYNFIKKVGNRKTYLALKRSDLWFSDRLTNKCKPLL
                     VDLEKETAVYFDRSLTLYFEDFKNHTLQTWPLAEGHDEAVQLDYATNIKQKKVWGVST
                     FDNKHLTISVWEIK"
     gene            11980..13194
                     /locus_tag="PKY09_08045"
     CDS             11980..13194
                     /locus_tag="PKY09_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB12846.1"
                     /translation="MLTFAQLMNTVVAIIFWISLLGLLHTYVLFPLLMRLLLKLKTEV
                     KEEVADNLFKPMVSVVMAAYNEEKVIIQKLESVIKSNYPSDKLEIIVGSDCSNDGTDV
                     LVQEFSQNHPQVKFIRFNSRTGKPAIINQLVKHSKGEFLIITDANVMFDAEAISSMMN
                     RFTVAEVGLVDSNMVNIGLMKSGISSQEKQYIQTEVLTKYAEGEIWGSMMGPFGGCYA
                     IRKSLFSDIPANFLVDDFYVCMKILEQGYHSVSSLKAIVYEEASHNLWIEYRRKTRIA
                     TGNFQNLAKFSKMIFKGRGIGFCFMSHKVLRWKGPVLMLLLFFSSFVAWWLSVGINPV
                     LCLGIFGAMSLSLMLLLADRLLNLFQIHVGLLRLNTHFLIMNAAMLKGMFRYFKGVKS
                     GVWQPTSRTQNA"
     gene            13247..13636
                     /locus_tag="PKY09_08050"
     CDS             13247..13636
                     /locus_tag="PKY09_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB12847.1"
                     /translation="MITTPLKIFIVDDDNMMLEMLRDRIGQHASNTVLTFSTGEDCLK
                     HVAENPDVIILDYYLNSKNPDASNGMEILKAIKKYNPNIHVIFLSGQERYGVAMQTLQ
                     KGAEYYVIKDNDAFDKIEKILSGITVS"
     gene            complement(13688..14143)
                     /locus_tag="PKY09_08055"
     CDS             complement(13688..14143)
                     /locus_tag="PKY09_08055"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNB12848.1"
                     /translation="MMNETVRVTKIFVFDMAHALYGYDGPCKNIHGHTYKLSVTLKGT
                     PVNDKSNPKAGMVIDFSILKQIVQEKIIKQFDHALVLNNDSPHKLLDKALTQQFEKII
                     YLDVQPTCENLLLHFKNQLVSHFTDKIKLVFLKLEETPSSYSEWFVDDN"
     gene            complement(14143..14652)
                     /gene="paaJ"
                     /locus_tag="PKY09_08060"
     CDS             complement(14143..14652)
                     /gene="paaJ"
                     /locus_tag="PKY09_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNB12849.1"
                     /translation="MSSVKNTESSKSTESLIWALLHTVPDPDIPVISIVDLGIVRNVE
                     WKNEIAIITITPSYSGCPAMSVFVDDIRQVLNGAGFQSEIKTVLSPAWTTEWISQSGL
                     DKMKAHGIAPPEDESGMDSIFAMQKPVTCPRCNSKQTKMLNRFGSTPCKALWYCNECS
                     QPFEYFKCH"
     gene            complement(14664..15422)
                     /gene="paaC"
                     /locus_tag="PKY09_08065"
     CDS             complement(14664..15422)
                     /gene="paaC"
                     /locus_tag="PKY09_08065"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNB12850.1"
                     /translation="MKTKDALYNYCLRLADSNMIHGQRLAEWCSRGPFLEEDIALSNM
                     SLDMFGQAEMLYEYASTQSEAEHSADDLAFRRSEREYFNCLLVEQENGDFAQTMMKVF
                     LYSAFAKQLYESLLNSNNETLSGFAAKAIKEVKYHLRHSSEWLIRFGNGTDESKRRVQ
                     QAVTNLWRFTQDMFEMNDADAALVDNGISFTTEALSAKWQLNVNEIFTAANINIPETN
                     NTITGGYNGIHTEHLGVMLCEMQYLQRAYPDAKW"
     gene            complement(15425..15715)
                     /gene="paaB"
                     /locus_tag="PKY09_08070"
     CDS             complement(15425..15715)
                     /gene="paaB"
                     /locus_tag="PKY09_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNB12851.1"
                     /translation="MKNDTQFPQWEVFIQTKNGGPYEHQGSLHAADKKMALQNARDLY
                     TRRNEGRSIWVVPTECIAATTADDAADFFDPANDKIYRTPNFYKMPQGTKNL"
     gene            complement(15727..16674)
                     /gene="paaA"
                     /locus_tag="PKY09_08075"
     CDS             complement(15727..16674)
                     /gene="paaA"
                     /locus_tag="PKY09_08075"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNB12852.1"
                     /translation="MTEEEKEKQFQQRIDAGEKIEPRDWMPEAYKQTLFRQISQHAHS
                     EIIGMQPEGNWLLRAPSLRAKKILLAKIQDEAGHGLYLYSAAETFGIDRAEMIAMLHS
                     GKAKYSSIFNYPTLNWADMGAIGWLVDGAAIVNQTMLAKCSYGPYSRAMIRICKEEGF
                     HQRQGYEIIAKMMNGTTAQRNMAQDSLNRWWEPSLMMFGPHDSESTHSKDSMKWKIKV
                     ESNDTLRQRFIDRTVEQAHHIGLTIPDKNLKYNKATMHWEISPINWDEFWNVVKGNGL
                     CNYQRMSHHIRYHNEGKWVRDCAAAFEKSREKENSSISI"
     gene            complement(16685..17845)
                     /gene="hppD"
                     /locus_tag="PKY09_08080"
     CDS             complement(16685..17845)
                     /gene="hppD"
                     /locus_tag="PKY09_08080"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNB12853.1"
                     /translation="MESNDLTSVKNFNYSETTKIFSQAQDFLPLLGTDYVEFYVGNAK
                     QAAHYYKTAFGFQDLAYAGLETGVRDRTSYVLQQGKIRLVLTTPLTSANPIADHIKKH
                     GDGVKVIALWVDDATDAFEQTVKRGAKPYLNPESIKDIDGEVCRSGIHTYGDTVHIFI
                     ERKNYKGVFLPGYEKYQSDYNPPSTGLKYIDHMVGNVELGGMNVWSKFYAEVMGFFNL
                     ITFDDKDISTEYTALMSKVMTNGNGYIKFPINEPAIGKKKSQVEEYLDFYEGAGCQHI
                     AVATDDIVYTITEMRKRGVEFLYVPGSYYDTVKERVGIIEEDLDELKKWGIMVDRDEE
                     GYLLQIFTKPVEDRPTLFYEIIQRKGAKSFGKGNFKALFESIEAEQARRGTL"
     gene            complement(17933..19108)
                     /locus_tag="PKY09_08085"
     CDS             complement(17933..19108)
                     /locus_tag="PKY09_08085"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNB12854.1"
                     /translation="MPIYQRQGKTPNKRHIVFRQSNGSLYHEELFGTEGFSGISSLVY
                     HLNPPTMVKDHGKPYSVRPEIAVEDNLQARSFLGFEVAPENDYLKSRKVLFVNDAMQI
                     GLASPMKGTKDYFFKNADADEMLFVHRGSGILKTMFGSIDFEYGDYLVIPRGIIYQIN
                     FTTTDNKLLFIESHAPIETPARYRNQYGQFMENSPFCERDFKLPHSLQTHDERGEFLI
                     NIKKRGLIYPYIYETHPFDVVGFDGCFYPYGISIFNFEPITGRIHMPPPIHQQFQSSN
                     FVVCSFVPRLYDYHPQAIPAPYHHSNMDSDELLYYVDGDFMSRNNIRQGQITLHPGGL
                     SHGPHPGAIERSIGKKETNELAVMIDPFSPVKITKAATEIEIEEYYQSWRMHESEAI"
     gene            19372..20958
                     /locus_tag="PKY09_08090"
     CDS             19372..20958
                     /locus_tag="PKY09_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12855.1"
                     /translation="MKNRNLPYQDFKLVALPNKIIKVLADGNFSQLNTVDMEQLFHAL
                     MFLLAYPQSEKQESFTLKKLNTLTNAIKNKTTAFKNKLQNSGLPFTNMSVQFTHDCII
                     WLQQQPDIKVAFQNFVNDEVDLSGLLSLTLNVAERAETSAGLNAGDLLVSLDVKQPLI
                     LDFLLNEFQKLSQPLVKDYLFDKAGLQCTIKPKSKFFSLAYNRFIRSKTFYHEEILKK
                     FDYKSVLDSEINDAVSLSEADELALIKTIKFAMALTNRETDTVTYMDETTLQYFELER
                     GISVAIYGMTSQRQLPFESYIGYTLFKNGFPYAYGGGWVFGQRAMFGINIFEWFRGGE
                     SGFVLCQLLRVYKQVFGVERFEVEPYQYGLDNPDGIASGAFWFYYRFGFRPVDSTLRK
                     LAAAEFEKITKKKTYRSSSKTLLRFTESIIELSLHCSQKVTIEKVTGNISKMIRSRFK
                     GNRLLAEQTCMNSFLDKLKKEKITYNNQTNFTEVALWSMAFDLKQKQELQMLADMAFI
                     KPIDPYRYQALLLKLLRNLT"
     gene            complement(21021..21785)
                     /locus_tag="PKY09_08095"
     CDS             complement(21021..21785)
                     /locus_tag="PKY09_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12856.1"
                     /translation="MIKRLLTIAFAVITTNVFSQVLVSYDFTGYNGLPASIPAGWTIT
                     VNDTSAAGKTFYTTAGSCATSCPSYKFNQTGATIITPSFSNATHLKFFMKGNGNTQLN
                     PFKVYTTADGTNWTNIQTYSPVSLTGSFYTLSLNTSDIQVKFEYIKDSLGFNVALDDI
                     TISNGPVGINNISSESLNIYPTISTGAINIESSAEKKAIEISVINVIGKEVKRFNFIQ
                     NNGKSIINLSELPDGVYVLKMTLNGQPFTKRVVIKR"
     gene            complement(21904..>22545)
                     /locus_tag="PKY09_08100"
     CDS             complement(21904..>22545)
                     /locus_tag="PKY09_08100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNB12857.1"
                     /translation="YWGLTDAAHQSVQKELKNARVIFDVGANIGYTSLYYASMNGTAQ
                     IFSFEPHPDIFIRAQENIALNTFQNINLINLGLGEKKEELKLYEVNEHNPGMNRIIRE
                     EKAIAFKKINVDTLDQFVAEKNIEHLDLIKIDVEGFEYAVINGGRGAIQKYKPLLFIE
                     LDDNNLKENNSSALALINLLCSLGYTTFYRTDNMSALSTNTDFSHCHYDMVAK"
BASE COUNT         6853 a         3844 c         4457 g         7291 t
ORIGIN      
        1 aattgcacag atgaaaaagg gtgctacact catttcattt atgtgggctg ctaccaatgc
       61 agatttagtg gaagcttgta agcaagcagg tatcaatgcg ttttcaatgg atgctattcc
      121 acgtatttcc agagcgcaga aaatggatgc tttaagttct caggcaaacc ttgccggata
      181 taaagctgtt attttatgcg cagatacatt aggtaaaatt ttgcctatga tgacaactgc
      241 tgctggaacc attcgaccct caaaggttgt aatatttggt gccggagttg caggcttgca
      301 ggcaattgct acagcaaaac gattgggggc agttgttgaa gttagcgaca tcagaccgga
      361 gactaaagaa caggttcagt cactcggtgg aaagtttatt gaagtaaaag gcgatgacag
      421 tataaaaatt gaaggcggat atgtgaaagg tgtttcagaa gaattcttga aaagacaaca
      481 ggaattagta gccaaacaca tcaaagaagc agacattgta attaccactg cattaatccc
      541 cggtaagaaa gcgccattgt tagttacaga ggagatggta aagagtatga aaaatggttc
      601 agtcatttta gatatggctg ttgagcaggg aggaaatgtt accggaagtg agttaaatca
      661 aaccgttcag aaaaatggtg tgacaattat tggtgaaagt aatataccaa gtttgttgcc
      721 gatgaatgca agtgatttat atgcaaagaa tattcagaca tttttacttc acttaaccac
      781 aaaagacgga tttaagtggg agatggaaga agaaataaca aaaggaagtt taattgtaaa
      841 atcttgattc aataattaaa agcaaaaaaa acatgactga aataatcaat tttataggaa
      901 cacatcagga gattatattt tttattattc tttctgtatt tgtgggtata gaagtcattg
      961 gcggagtacc gacagtatta catactccac tgatgtccgg tgccaacgcc atacatggag
     1021 ttgttattgt aggagctata atagtaatgc tcaatgttga tgccaatgac tatgtagcac
     1081 tgtcattagg atttttggct gtagtgctcg gtacactcaa tgtggtgggt ggatttgttg
     1141 taacagaccg catgttggaa atgtttaaga aaaaataaaa ccacaaaaaa gaatttaaga
     1201 aatcagcgca tgacacctga agtattatat aacattcgta caattacata cattattgca
     1261 tctgctacgt ttatcattgg acttaaacgt atggggaatg ccaagactgc tcgtaacggt
     1321 aatctcattg ccgcagtagg tatgacttta gcgattattg catcaatatt tattcatgtc
     1381 aataaagtac aaactgaaac cggtgtagca tttgagcagg ttgaaacacc aggttttatt
     1441 tacagcttaa tttttgctgc aatcattatc ggaacaatca ttggctggct tactgccaaa
     1501 aaagttcaga tgacaaaaat gcctgaattg gtatccatgt tcaatggtat gggtggtgcc
     1561 tgtgctgcgc ttattggcct catggagttt catcataata caggaaacac aggtgctttg
     1621 atagcaattg tacttgggtt gattattggt agtgtgtctt tttcaggaag tgtaattgcg
     1681 tttttaaagt tgaatggtac catgaacaag ccagttcgtt taccatcgta caacattata
     1741 aacacactgg tcatgatagg cgtatttggt ttcggtgcat acatgatttt tagtggaaca
     1801 gaaagttcaa cattgttgta cttattgttt gcagcagcat tgatttatgg aattttattt
     1861 accattccaa ttggtggtgc cgatatgcct gtagtaattt cattgcttaa ctcattcaca
     1921 ggattggcag cagcattcgg tggtttcctt tatgataata aagtgatgct aacaggaggt
     1981 attcttgtag gttctgcagg aaccttgtta acattagtga tgtgtaaggc aatgaaccgt
     2041 cctttgagca atgtaatatt tggagctttt ggtggaggag gtgcttcagc aggtgatgga
     2101 cctgatgtta aaggaagtat taaggatatg tctgttgcag atgctgccgt attgcttaat
     2161 tattctaaga aagttgtaat agttcccggc tatggattgg cagtagcaca ggcacaacat
     2221 atcattcacg aactggaaat gttgttggaa gaacgtggtg ttgaagtaaa atatgctatt
     2281 cacccggttg caggtcgtat gcccgggcac atgaatgtgt tgctggcaga aagtaatgtt
     2341 gattatggta agttagtgga gatggaagag ataaatcctg aactggaaca aacagatgta
     2401 gtgttggttg ttggcgcaaa tgacgttgta aatcctgcag caaaaacaga tccatcatca
     2461 ccaatttacg gcatgccaat attggaagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnc attaaaataa ttctgtaaat aaactttttg ctcactcatg ccaaggtgct
     2641 gaatagaaac taaaatttct tttagctttt tagtcattaa ttttttgccc tgttcaccac
     2701 caaactgatc agcataacca tctgaaaaaa gatatatggt atctttttgt tgtattttaa
     2761 tctgatgatt tgtaaactga ttgttgtgtt ttatttgcaa tccaccaata ggaaatttgt
     2821 ctgctgcata aacaataagt tcactatttc tgattaacca caatggtctg ttggcgccag
     2881 caaattctaa actcattttt tctatatcaa gagaacagat agaaatatcc attccatcat
     2941 gtgtttcgct ttcatgttgt ttgagcgaat gaataatgcc ttcgttcaac gcgtctaaaa
     3001 tacctgaagg tgtagtgatg tttttttcgt tgatgataga tttaatcaat gaacttccga
     3061 taatactcat aaatgcaccc gccacaccat gtcctgtaca atcggctgcg gcaataatgt
     3121 gcttgttgtt tttctgtgta aactcataaa aatcaccact gacaatatct tttggaatat
     3181 agagaataaa tgattggtca agtattttgt aaacggtatt taagtttgga agaacagcgg
     3241 cttgaattcg tttggcataa atcagactat cagtaatttc tttgtttttg cttaggattt
     3301 ctttgttttt ttcttcaagt tcaaaggttc tttccgttac tttgttttca agtaaaatgt
     3361 tttgttgtgc cagaatattt ttcttttcgg tttccttcag cagattttct tccccaagtt
     3421 ttttaatagt aacaacttgt tcaatgagct gctgttgatt ttgtccgtag cgtctggcaa
     3481 gtacaatggc aaccattata ggataaatca atgggcttat tgtaaaaaat aaagtcaaaa
     3541 tatctgagga ggtattttgt tgatacatat tcttggaagc cgtatatatg attggaagaa
     3601 taagtaatat cagccctaaa gccgttccat aagttagcac tttaacatcc cattttttct
     3661 gaccaaaacc ttgtacccag tggtatgcaa aagaaaatat agagtaagaa aaaaagatta
     3721 agctgctcag taatgtaatc acaggaactt caatgttgcg aacaaaatta attgtaaaga
     3781 tgatgtcgtt aggaataagt aaagcaatta acatcaccag acacatgatg agaagtggcg
     3841 tttttgtttt attaatattg tttatagcta atgccatgaa aatagccagc aattccaaag
     3901 aaatcaaaaa agaataagtt gaaattacac caacaacggg tgattgagtt aaaacattgg
     3961 ttaggtactg aatcgcagtg gctaaacaat aaagaccaaa aaacaaatat tctttatttc
     4021 tgataaagaa gtaaaataaa atcaagacta cagcaaacga aaaataaaac gtagcaacag
     4081 cataatgtct gacctcatct ctttgatttt ccaattgatt ttcttttgcc catttctctt
     4141 ctcctaacct taaatgaagt gaaaatattt ttgaattctg attaggcaga taaagaactt
     4201 gcagattatt tttgggttgt gttaaaacaa aacttatata gttctctttt agcgattgaa
     4261 gatctgtgta ttttgtagag ttattaaaaa ttccgtcaga tgtaatggta gtattgttca
     4321 gaagcacttt aaccgaacca ttagtactat aaaaaaggta tttcacttta ttgagcatgt
     4381 tgctgtcaac taaaaatgtt gttgagagta aaagtccttc aggctcatga ttacgaaaca
     4441 taaatgtgtc aatgctggtt gttgtaaagg agttaacaat gccttcttta ctataaactt
     4501 taacacgcca cttaaagttg tttattacat tcagactata aatggtatct tgcttagccg
     4561 gctttgatcc aaaaaatgta agtgtatctg tgttcgcaaa agatgtaaaa tagctgagaa
     4621 aaagaagaaa cgatagaatg tatctgatat ttattttttt tagatttaaa ttaaagggca
     4681 tatttgcaaa attacagggg ctaatttaac tatattagca atctgacaaa taccacaatc
     4741 ataaaatcaa aaaaaatact aaactaaata gttaaattaa atgttagaat aggatgatta
     4801 cagttattat accaacactc aacgaagaag agaatgttgc caatgtgata aaatttgcaa
     4861 agcagtattc attagtaaca gaggtgcttg tagttgatga caagtcaatt gataacactg
     4921 taaggattgc tcaggatagt ggtgcacgtg tcattacaag tactcgaatt gggaaagggg
     4981 gctctatgcg tgaaggggtg atgtttgcta ccaatgaagt ggttgttttt ttagatgcag
     5041 acattaaccc ttatcctcat tttacaatta aactgttaac agaaccaatt ttacaaggtg
     5101 gtgctgattt tgtgaaatct tcttttaaca gaaatgcagg tcgtgtaaca gagcttgtag
     5161 caaaaccaat gttgagtatt ttttttcctg aactgttgca atttcagcag cctttgagtg
     5221 gtatgatagc cagtaagaaa tcattgcttc aaaagttaga cttcagagaa gactatggtg
     5281 tagatatagg gttactaatt gatatgcact taatacatgc taatattcag gaggtagata
     5341 ttgggtatat tgaaaataaa agtaaaccct ggcaggcttt gggaaaaatg agcaaagagg
     5401 ttgcacaaac gatcatctta aaagcctcat catccaacag tccgcactat aattttgaag
     5461 agtttggcgt gttaaatgaa attaggtcac aaatggattt tgcattggca agtcagcaag
     5521 aatcgctcag caaactgatt gtttttgata tggataatac aatactgcga ggacggttta
     5581 ttgatacttg tgctgaaaaa tttaatttta agacacagct aatggatatt cgctcttctg
     5641 aaacagatag taccatcctg accaaaagaa ttgccaagct tttaaagaat agaactattg
     5701 atgaattgat tgatgttgca gacagcattg atattgttgc agacagtgag aaggttattg
     5761 ataacttaaa gactcagggt tatgttgttg gtattatttc tgatagttat gattgcatca
     5821 ccaatcatgt taaaaataaa ttaaggatgg atttttcatt gtcaaacgaa ttggagtttt
     5881 caaaaagtat atgtaccgga gaagtaaaaa tcccatcttt ttttgtaaac aatgaaaaca
     5941 gctcatgcaa gcatgtatta tgcaaaacca atgccctcca taaactttta gagaagtata
     6001 atattaaaaa agaaaacagc gtagcaattg gagatagtat gaatgatctt tgcatgatta
     6061 aggaagcagg cttaggaatt gctttttgct ctaaagatga gctactcaac caccatgcag
     6121 atgtgattat tcggaaaccg gaatttagcg aattattgaa tcttactaaa taagttgaac
     6181 taaaaaaaat aattgaaaaa caccgtttac gaaatcaaca gacttgcttg ttttaaaaac
     6241 gataccccat agcaaagctg aattttacac tgggcgaaat cggggtggta tcaaaactgt
     6301 cattgttagt ttcttcgtct tcactggcag aatcaaaata gtttatgcca atgcccagat
     6361 ccatacctaa atacagggtt ttagaaattg taacatttaa gctgttgttg atcataaagc
     6421 ctaattgtgt atggttgtcg gaatgcttgt atcttgtgta aatatattga tttccattcc
     6481 aaacactttc tgtacggcta tgttccactt gtccacttgc aaaataaaat tgaggtccta
     6541 cagcatagcg caccacacct tgctttgtag gataaagttt tatggtaggc ataaaataaa
     6601 aaaatcccgg atttcgcagt gacaaattca caggaagctg aatgccaatt aatccattgg
     6661 aaacaattct ttcataattt attcctattg caacatcagt gttgttgtcc ataggttcgt
     6721 tagaaataat cgtcatgggt gtaatggaga aaatattgtt gcctaactcc ggtttttcct
     6781 gtgcgaataa cattgtagaa ataagcagga gcattgatga tagtattgtt ctcataattt
     6841 atttataaaa gcagtttttt gaattgtata aaatcagtac aaaaatagca gagttttcga
     6901 gaaaatagtt gggcagttac tttcagattt taccggtaat ttatactttt gtaaagaact
     6961 aaaagtgaga ttatggccga tcagacagtt gttacagaaa acaaggagct ttcatttgaa
     7021 gagttcagac agagggtact taacgactat cgtttaggac gtatcagcag agaaaccagt
     7081 ttgttgggac gcagagaagt gctgacaggc aaggcaaagt ttggcatttt tggcgatgga
     7141 aaagaggtgg cacagatagc tatggctaag gttttccaaa atggcgattt ccgttccggt
     7201 tactatcgcg atcagacatt tatgatggca gcaggcttac tcacagttca ggagtttttt
     7261 gctcagcttt atgcgcatcc ctcagtagca gcagagcctg caacagctgg tcgcgccatg
     7321 aatggacact acgcaacacg attactgaat gatgatggca gttggaaaaa tcaaacacaa
     7381 tctaaaaatt cttctgccga tatatcacca acggcaggtc agatgccacg attactgggg
     7441 cttgctttag cttctaaatt ctatcgcaat aatccagaac tcaaatcgga taagtttaaa
     7501 aatttttctg ttgatggcaa tgaagttgct tttggaacta tcggtgatgc cagcacttca
     7561 gaaggcttgt tttttgaaac aattaatgct gcaggtgttt tgcaggtgcc aatggttatc
     7621 agtatttggg atgatggtta tggtatttct gtacccaata aatatcagac aacaaaacaa
     7681 agtatcagtg aagcattgca aggatttgca cgtactcaag aaaaacccgg ttacgaaatt
     7741 atcaaagcta agggatggga ttatgcagag ctgtgtgatg cctatgaaca agcagctaaa
     7801 attgcacgta cagagcatgt gccggttatg gtgcatatac aacagatgac gcagcctcag
     7861 ggtcattcaa catcaggatc acatgaacga tacaaatcta aagaacgttt gatgtgggaa
     7921 gaagagtttg actgtcttag aaaaatgcgt gagttcatgg tgcaatctgc tattgcaact
     7981 gagcaagatc ttgatactat agatactgaa gctaaaaaat ttgttaatga cggtaagaag
     8041 caggcatggg atagctttac tactgaaatt aatgctgaaa gacaacaggc actatcactg
     8101 gcagaagcag caataagtag aagttcgaat ggtgtttttc tgaataaggc agttgaagag
     8161 attaagagta tcaccaattg cgaaagaaga gatattgcgg tttttgttca acaagtgttg
     8221 tttacactac gtggtgaaaa tagtgctgag ctggaaacac tgaagcaatg gtaccgccaa
     8281 tggcatcaat tgaattttga tcgttacagc tcacaacttt atgcagaagg tattggcagc
     8341 gcattgggaa ttgccgaagt aaaggctgtt tattctgcag aggcaaacat ggttgacggt
     8401 cgagaggtac ttcttgcaaa tcatgacatt ctttttagtc gctatccgga atacattgct
     8461 tttggagaag atgtaggaac aattggtggc gttaaccaaa cttttgcagg catgcaggca
     8521 aagtacggtg agcaccgtgt ttttgatgta ggtatccgcg aagcaacaat tgcaggtcag
     8581 ggaataggaa tggctgtgcg tggcttaaga cctctagcag aaattcaata tctcgattat
     8641 ttggcttatg caatacaaat tttatcggat gatttagctt gtttgcgcta tcgcaccaaa
     8701 gcaggacaga aagcgccact cattgtcagc actcgtggac acaggttaga aggaatatgg
     8761 cattcaggtt cacccatgca gtttattctt ggtgctttgc gtggcatgct tgtacttgtt
     8821 ccacgtaaca tgacgcaggc agccggattt tataatctgt tgttgcaatg tgatgaccct
     8881 gcattagtga tagaaccgtt gaatggctac agactaaaag aaaaactccc tcaaaatctt
     8941 ggtgaattta cagtgccggt aggtgtgcct gaaattcttg aagaaggtaa tgatgttact
     9001 cttgtaactt acggatcatg tgttagagtt gctcaggagg caatgaaaca actgcagttg
     9061 tgtggcataa gcgtggagtt gatagatgta cagtcactta taccatttga catccatcac
     9121 atcattgcag aatctattca aaaaacaaat aaggttgttt tctttgatga agatgtgccg
     9181 ggcggtgcaa cagcatacat gatgcaacag gttatagaaa atcaaaaagc atttgaattt
     9241 ttggaaacac ctcctgtaac ccttacggca aaagaacatc gtccggcata cggaagcgat
     9301 ggagattatt tctcgaagcc taatgctgaa gatgttttta atacggtgta tgaattaatg
     9361 cacgctgtaa atccgcaggc atttccatta ttcagataat cagcgtaagt ttctttttgc
     9421 tatccgatta gtttatcggg attttctgaa ataaattttt gccatccttt gcttttactt
     9481 ccggaaagct gactttgccc tgagtttttc tgaaagtgat gacatactgc agcagccagc
     9541 ccatcggtag catctaaata tttaggcatt tcctcaaaat gcaacagttg ctgaagcata
     9601 gcagctacct gctccttggt ggcactacca tttccggtga cagattgttt gatttttttt
     9661 ggactgtatt caaatacagg aatatttttg aatagagcgg cagcaagtgc aactccctgt
     9721 gccctgccca acttaagcat agactgcaca tttttaccaa aaaacggagc ttcaatagcc
     9781 atttcatcag gcaaataact ttcaatgagt cgtagtgttt tctcaaaaat atgtttcagt
     9841 ttcagtggat gattatcaaa cttttcgaga tgaataatac cggctgtctc taatttcatt
     9901 tctgttccgg aaataacgag tactccatat cccattacat tggtgcccgg atctattcct
     9961 agaattattt tttcgctttc tgacatgaac gctgttactg cttatttttt aaacttgcac
    10021 tataatctgt aattgcttgg aaggttcaaa aataaagatt aaaagtgtgc tacagttttt
    10081 actgaaagca gcaattgcat tgttttctct ttggttcatt gttcataaag ttatacaccg
    10141 tgagaatgag atgtcgtttt tcggttttgt gaatacaagg atacatacag aaggtgttta
    10201 ttccatactc atatctgtaa ttatattgat ggtgttgaac tggagtattg aggctataaa
    10261 atggaagttc ttaataaata aactcaccaa actaagtttc gttagaagcc ttagtgctgt
    10321 ttttgcagga gctacagtca gtttttttac accaaatcgt gtaggtgact atgcaggcag
    10381 aatgctcttt ttgcctgtat cagttcgtat agcatcgctg ctttcaactt ttgttggaaa
    10441 tatcagtcag cttattgtta ccttatgttg tgggcttgct gcaatggctt tgtcattaca
    10501 acattatgtg caatggaatg aaagtttagt tgttacaaca cgtgttgctt catttgtttt
    10561 tgcattgata ctttttattt cttttccata tataagagtt gtttttaaaa tgcctttgct
    10621 gaattttgtt cgacagcgat ttcctgaata tgtcatgcat cttgaaaatt attcaaacaa
    10681 agaaatgatg caaatacttt ttctatcctt gctgcgctat tcagtttttg cttttcagta
    10741 catgttatta tttctagttt ccggtattcc ggtggttcca tccatgatgg tcatagtagc
    10801 actaatcttt tttattcagg cattggttcc aacacttgca ctgacagagt ttgccataag
    10861 aggatcagtt gcattgatgg ttgtagcacc atttattcaa aatgatgagg ctgttcttat
    10921 ggcatcattt gcactctggc taattaatct ggcagtgccg gcagcaatag gctcactgat
    10981 gattttttac tttaatttca gaaaagagta acgcgcacta tttgatttcc caaactgaga
    11041 ttgtcagatg tttattatca aaagtgctaa caccccatac tttcttttgt ttgatgttgg
    11101 tagcataatc taactgcact gcttcatcat gcccttcagc caaaggccaa gtctgtaaag
    11161 tgtggttttt aaaatcttca aaatataaag tcaatgaacg atcgaaatat actgcggttt
    11221 ctttttccag atcaactaaa agtggtttgc atttatttgt aagtctgtcg ctgaaccaaa
    11281 gatcgctgcg ctttaatgca agataggttt ttctgttgcc aactttcttg ataaaattat
    11341 aagcaaactg atagatatag ccatcctgat agttgagcca aaggttataa gaataatgaa
    11401 accgtgtttt ataaaaatcc tggtagtagt cgggattgtt gtagtaagta ggaatagaag
    11461 ctatttcaaa aaagcgataa aaatgattca ggcttttata ccagtcttcg ggtttgttgt
    11521 aagcaatgat ttctattttt tttgttttac ggacatattt gaagattcgg gtttcttcat
    11581 tgtccatcat catcaggtta tcaaactcat caaactccat attacgcaga caaccttcat
    11641 agggaagttt aatttcttca agtatggttc cccgattgtt aattaaaaaa acataaggct
    11701 gattacagga gttgaatgca attatttcat cggcatctac agcaattcta taactaaaaa
    11761 tgccaccgtc aatcactctg tcgcggtcaa gtgtaaaaag gtctgtttta acaaaggggc
    11821 cacgcctttc ctgactataa ccggaatagt gaactgtaaa aagaataacg aaagaaataa
    11881 taaaccgcat gcaaacaaaa attaaggctg caaaagtcct ctttttattc atataaagcg
    11941 acaaaaagat aagtaaaatg tacattattt gaagttaaaa tgttgacctt tgcacaattg
    12001 atgaatacag ttgttgctat tattttctgg atttcgctat tgggactttt gcatacttat
    12061 gtgctgtttc cattactcat gcgtttactt ttaaagttaa agactgaggt aaaagaagaa
    12121 gttgcagata atttatttaa gccgatggtc agtgttgtga tggctgctta caatgaagag
    12181 aaagttatta tacagaagct cgaaagtgtc atcaaaagca attatccatc agataaattg
    12241 gaaataattg taggctccga ttgttccaat gatggtactg atgtgttggt gcaggaattt
    12301 tcacaaaatc atccgcaggt aaagtttata cgattcaaca gcagaacagg gaaacctgct
    12361 atcatcaatc aattagtaaa acatagtaaa ggtgagtttt taattattac tgatgccaat
    12421 gtaatgtttg acgctgaagc aatttcatca atgatgaata gatttactgt agcagaggtt
    12481 ggattggttg atagcaacat ggtgaatata ggattgatga aaagcggaat ttcatcacag
    12541 gagaaacagt atattcaaac agaagtctta actaagtatg ccgaaggtga aatatgggga
    12601 agtatgatgg gaccttttgg cggatgttat gcaataagga aaagtttatt cagtgacatt
    12661 ccggcaaatt ttttggttga tgatttctat gtatgtatga aaatactgga gcaaggttat
    12721 catagtgtaa gttcgcttaa agctattgtt tatgaggagg catcgcataa tctttggata
    12781 gagtacagaa gaaagacgcg tattgccaca ggaaattttc agaatctggc aaagtttagt
    12841 aagatgattt ttaagggcag aggtattgga ttttgtttta tgtcccataa agttttaaga
    12901 tggaaaggtc ctgtgttgat gttgctttta tttttctcat cttttgtggc ttggtggttg
    12961 tcagttggca taaatcccgt tttgtgtctt ggaatttttg gagccatgtc tctaagttta
    13021 atgttgttgc tggcagacag gctacttaat ttgtttcaaa tacatgtagg actgctcagg
    13081 ctaaatacac attttctgat aatgaatgct gccatgttga agggcatgtt cagatatttt
    13141 aaaggagtga aaagtggtgt ttggcaaccg acaagccgga cacagaacgc ctaattatat
    13201 attataaaaa aattgtaggt ttgtattcca tttgacacta aacaaaatga taacaacacc
    13261 attaaaaata tttattgttg acgatgataa tatgatgctt gaaatgcttc gtgacagaat
    13321 tggacagcat gcatcaaata cggttttaac tttcagtaca ggcgaagatt gtttgaagca
    13381 tgtagcagaa aatcccgatg ttattattct tgactattat ctgaattcaa aaaatcctga
    13441 tgcatctaat ggtatggaaa tattgaaagc tataaagaaa tacaatccaa acattcatgt
    13501 aatatttctt tcaggacagg aacgctatgg tgtggctatg caaactttac aaaaaggagc
    13561 agagtattac gtcattaaag acaatgatgc ttttgataaa attgaaaaaa tattgagcgg
    13621 catcacagtt tcttaaatca acctttaaaa aatttgacac tgatttttat atagcacaac
    13681 tcatttatca gttgtcatca acaaaccact cagaatagga agaaggagtt tcttccaatt
    13741 ttaagaatac caattttatt ttatctgtaa agtgagatac aagttgattc ttaaaatgta
    13801 acaacagatt ttcacaggtg ggctgcacgt ccaaataaat aattttttca aactgttgtg
    13861 taagtgcttt gtcaagcagt ttatgtggcg aatcgttgtt gagtactaaa gcatgatcaa
    13921 attgtttgat aattttttct tgtactattt gcttcaatat cgaaaagtca atgaccatgc
    13981 ctgccttggg attacttttg tcgttgacag gtgttccttt taaggtgaca gaaagtttat
    14041 aggtgtgacc atgtatattt ttacatggac catcgtagcc atacaaggca tgtgccatat
    14101 caaacacaaa aattttagtt acccgaacag tttcattcat cattaatggc atttaaaata
    14161 ctcaaacggc tgactgcatt cgttgcaata ccaaagagct ttgcatggtg ttgagccaaa
    14221 gcgattcagc atttttgttt gtttgctgtt gcaacgtggg caggtaaccg gtttttgcat
    14281 tgcaaatata ctatccattc cggattcatc ttcaggaggt gcaatgccat gggctttcat
    14341 tttgtcaaga cccgattgac ttatccattc tgtagtccat gcaggtgata gtacggtttt
    14401 tatttctgat tgaaaacctg caccatttaa tacctgcctg atgtcgtcca caaaaactga
    14461 catggcagga catcccgaat aacttggcgt gatagtgatg attgctattt cgttcttcca
    14521 ttcaacattg cggacaatgc ctaaatcaac aatgctgatg acgggaatat caggatcagg
    14581 aacagtatgc agcaatgccc agattaatga ttctgttgac ttgctgcttt cagtattttt
    14641 tactgatgac atgacttttt attctaccac ttagcatcag gataggctct ctgcaaatat
    14701 tgcatttcgc aaagcatgac acctaaatgc tctgtatgaa taccattgta accacctgtt
    14761 atggtgttgt tcgtttcagg aatgttaatg tttgctgctg taaaaatttc atttacattt
    14821 aactgccatt tcgcagataa cgcttctgtt gtaaagctga taccgttatc aactaaagct
    14881 gcgtctgcat cattcatttc aaacatgtct tgtgtaaacc gccacaaatt tgtgacagct
    14941 tgttgcacac gtcttttgct ttcatcagtt ccgtttccga atcttatcag ccattcgctg
    15001 ctatgtcgca aatgatattt tacttccttt atagctttag cagcaaaacc ggacagtgtt
    15061 tcgttattac tgttcaacaa tgattcataa agttgttttg caaatgcaga atacaaaaac
    15121 actttcatca tagtttgtgc aaagtcacca ttttcctgct ctaccagcag gcagttgaaa
    15181 tattctcttt cactgcgtct gaaagccaaa tcatcagcac tatgctcagc ttcagactgt
    15241 gttgaagcat actcataaag catctcagcc tgaccaaaca tgtctaatga catatttgat
    15301 aaagcaatat cttcttctaa aaaaggtcct ctgctgcacc attcagccaa tcgctgacca
    15361 tgtatcatgt tgctgtcggc taatcgaaga caatagttat ataatgcgtc ttttgttttc
    15421 atgtttacag attttttgtt ccttgtggca ttttataaaa atttggtgtt cgataaatct
    15481 tatcatttgc cggatcaaaa aaatcagcag catcatcagc tgttgttgca gcaatacatt
    15541 cagtgggcac tacccaaata ctgcgccctt catttctgcg tgtgtaaaga tctcttgcat
    15601 tctgaagcgc catttttttg tcagcggcat gcaggcttcc ctgatgttca taaggtccac
    15661 cattttttgt ttgtataaat acttcccatt gtggaaactg agtgtcgttt ttcattcttt
    15721 gattttttaa attgatattg acgaattttc tttctctctg cttttttcaa atgctgctgc
    15781 acagtcacga acccattttc cttcgttatg ataccggata tgatgactca tgcgttgata
    15841 attgcatagg ccattgcctt ttaccacatt ccagaattca tcccagttga tggggctgat
    15901 ttcccaatgc atagtggctt tgttgtattt taagttttta tcaggtattg taagtccgat
    15961 gtgatgtgcc tgctcaacag ttctgtcaat aaaacgttga cgaagtgtat cattgctttc
    16021 aacttttatt ttccacttca ttgaatcttt tgaatgtgta gattcagaat catgaggacc
    16081 aaacatcatc agtgatggtt cccaccaacg gttcaatgaa tcttgcgcca tgtttcgttg
    16141 tgctgttgtt ccattcatca tctttgctat aatctcatag ccctgacgct gatgaaagcc
    16201 ttcctcttta cagatacgta tcattgcacg tgaataaggt ccatagctgc actttgccag
    16261 cattgtttga ttcacaatag ctgctccatc aaccaaccaa ccaattgcac ccatgtcagc
    16321 ccaatttaat gtagggtagt taaatatact tgagtacttt gcttttcctg aatgtaacat
    16381 agcaatcatc tctgctctgt caataccaaa agtttctgcc gctgaataga gataaagtcc
    16441 atgtccagct tcgtcctgaa ttttagcaag cagaattttt tttgcacgta gtgatggcgc
    16501 tctcaacaac cagtttcctt cgggttgcat tccaattatt tccgaatgtg catgttgcga
    16561 tatctgtctg aatagcgttt gtttatatgc ttcaggcatc caatcgcgcg gttctatttt
    16621 ttcacctgca tcaattcttt gctgaaactg tttttctttt tcttcttctg tcataacttt
    16681 aaaattataa tgttcctcgc cttgcctgct ctgcttctat actttcaaac aaggccttaa
    16741 aatttccttt accaaaagat ttggcacctt tacgttgtat aatttcatag aataacgttg
    16801 gtcggtcttc aacaggtttg gtaaatattt gtaatagata accctcctca tcacggtcaa
    16861 ccatgatacc ccactttttc agttcatcca agtcttcttc aataatacct acacgttctt
    16921 taacggtgtc gtaatacgaa ccgggaacat atagaaactc aacaccacgc tttcgcattt
    16981 ctgttatggt ataaacaata tcgtcagttg ctacggcaat atgctggcat cccgcaccct
    17041 cgtaaaagtc gaggtattct tctacttgtg atttcttttt tccaatagcc ggttcattaa
    17101 tcggaaattt aatgtagcca ttaccattgg tcatcacctt actcatcaat gcagtgtatt
    17161 ctgtcgaaat atctttgtca tcaaaagtta tcagattaaa aaatcccatt acttcagcat
    17221 aaaatttact ccacacattc attccaccca actccacatt gcctaccatg tgatcaatat
    17281 actttaaccc tgttgatggt gggttgtagt ccgattgata cttttcatat ccgggaagaa
    17341 agacgccttt gtaatttttt ctctcgataa aaatatgaac tgtatcacca taggtatgaa
    17401 ttcccgatct gcaaacttca ccatctatat ctttaattga ttcaggattg agataaggtt
    17461 tggcaccgcg ctttacggtt tgttcaaatg catcagttgc atcatcaacc caaagagcaa
    17521 ttactttaac accgtcacca tgttttttaa tatgatcggc aattggatta gcagaagtta
    17581 aaggggtagt aagcaccaag cgaatttttc cttgctgaag tacataagat gttctgtcgc
    17641 gtacacctgt ttcaagtcct gcataggcaa gatcctgaaa tccgaaagca gttttgtaat
    17701 aatgtgcagc ctgcttggca ttacccacat aaaattcaac atagtctgtt ccaagcagtg
    17761 gaagaaaatc ctgtgcctgc gaaaagattt tagttgtctc agagtagttg aagtttttta
    17821 cggatgttag gtcgtttgat tccatattaa aatttttaat tacccccgaa gctctgctct
    17881 cctttggtgg aggtgaaaaa tgagagagca ggagcagggg tatgttgctt ctttatattg
    17941 cttcagactc gtgcatgcgc cacgactgat aatattcttc aatttcaatt tctgttgctg
    18001 ccttagtaat ttttacgggg ctgaacggat caatcattac ggcaagttca ttggtttctt
    18061 tttttccaat gctgcgttca atagctccgg gatgcgggcc atgagataat cctccgggat
    18121 gcaatgttat ctgtccctga cgaatgttat ttctactcat aaaatcacca tcaacataat
    18181 aaagcagttc atcactgtcc atgttgctgt ggtgataagg cgcaggtatt gcctgcggat
    18241 gatagtcata caatcggggc acaaaggagc acactacaaa attgctcgac tgaaattgct
    18301 gatgtatagg cggaggcata tgtatgcgtc ctgtaatggg ttcaaagtta aagatggaaa
    18361 ttccataggg ataaaagcat ccgtcaaaac ctacgacatc aaaaggatgt gtttcgtaaa
    18421 tgtagggata aatcaaacca cgttttttta tgttgatcag aaattcgccc ctttcatcat
    18481 gtgtctgcaa ggaatgagga agtttaaaat ctctctcaca aaaaggagag ttctccataa
    18541 actgtccata ctgattgcga tagcgtgctg gtgtttcaat tggagcgtgt gactctatga
    18601 aaagcaactt attgtcggtg gtagtaaaat taatttgata gatgatacct ctgggaataa
    18661 cgaggtagtc gccatattca aaatcaatag aaccaaacat ggttttcaat ataccgcttc
    18721 cgcgatgaac aaaaagcatt tcatcggcat cggcattttt gaaaaaataa tctttagtac
    18781 ctttcatagg tgatgccaga cctatttgca tggcatcgtt tacaaaaagt acctttctgc
    18841 ttttcagata atcattttcc ggagctactt caaaacccag aaaactcctt gcctgaaggt
    18901 tatcttcaac agcaatttcc ggtcttacac tgtacggctt gccatgatct ttaaccatgg
    18961 tgggcggatt aaggtgatat actaaggaag aaattcctga gaatccttct gtaccgaaaa
    19021 gttcctcatg gtacagactg ccattacttt ggcggaaaac aatatgtctt ttgttagggg
    19081 tcttaccctg tcgttgatag atgggcattt caaaatattt ttgcaaagag gtcgaaaatt
    19141 cccgatgttt gatatgacat ttatcagtat gtagggtgat ttttggactt tatacaaagg
    19201 aaatacttgt caaataattg agttctcctc tttgtgtgga caaaaaagtt aagcccttgt
    19261 aataggagcg ttttttgtgg aaaatatctt ttagtttatt ccttaacgtg atatttgtca
    19321 gcactgtatt tactaactga ataataatta atttcttaat cttgtaaggt tatgaagaat
    19381 agaaatttac cttatcagga ttttaaatta gttgcactgc ccaataaaat tattaaggta
    19441 ttggcagatg gaaatttttc tcagctaaac actgtggaca tggaacaatt gtttcatgca
    19501 ctgatgtttt tattagccta tcctcagtcg gagaaacaag agtcgtttac tttaaaaaag
    19561 ttaaacacct taactaatgc catcaaaaac aaaacaactg catttaaaaa caaattacaa
    19621 aacagtggat tgccttttac taacatgtca gtgcagttta ctcatgactg tataatttgg
    19681 ttgcaacagc agcctgatat taaagtggct tttcaaaatt ttgtcaatga tgaagtagat
    19741 ttaagtgggt tgttgtcgtt aacactgaat gttgcagagc gcgcagaaac ttctgccgga
    19801 ctcaatgcag gtgatttact tgtttcgctt gatgtgaaac agcccttgat actggatttt
    19861 ctgctcaatg aatttcaaaa gttgtctcaa cctttggtaa aagattattt gtttgataaa
    19921 gcaggattgc aatgtactat aaagcctaaa agcaaatttt tttcattggc ttataatcgt
    19981 ttcatcagga gtaaaacatt ctaccacgag gagattttaa aaaagtttga ttataaaagt
    20041 gttttagatt ctgaaatcaa tgatgctgta agtttatcag aggcggacga acttgcactg
    20101 ataaaaacaa taaaatttgc tatggctctc accaaccgtg agacggatac tgttacctac
    20161 atggatgaaa ccaccttgca gtattttgaa ttggagagag gaatttctgt agccatttat
    20221 ggtatgactt cgcaaaggca attaccgttt gaatcttata taggatatac cttgtttaag
    20281 aacggttttc cttatgccta tggtggagga tgggtttttg gtcaaagggc aatgtttggt
    20341 attaatattt tcgaatggtt tcgcggaggt gaatccggct ttgtactatg tcagttgctg
    20401 agagtatata agcaggtatt tggtgtggag cgttttgaag tggaacccta tcagtatgga
    20461 cttgataatc ctgatggtat tgccagtgga gcattttggt tttattaccg ctttggtttc
    20521 agaccggtgg atagtacact cagaaaatta gctgccgctg aatttgaaaa aatcacgaag
    20581 aagaaaacat atcgttcttc ttcaaaaaca ttactgcgat ttacagagag cattattgaa
    20641 ctttcacttc attgttcaca aaaggtgact atcgaaaaag taacaggcaa cataagtaaa
    20701 atgattcgaa gccgttttaa agggaataga ttattggcag aacaaacctg tatgaatagt
    20761 tttttagata aacttaaaaa ggaaaaaatc acatacaaca atcagacaaa ttttacagaa
    20821 gttgccttgt ggagtatggc ttttgattta aaacagaaac aagagttgca aatgctggca
    20881 gacatggctt ttattaaacc cattgatcca tacaggtatc aggcattatt gttgaagttg
    20941 ttgcgtaatt taacctgatt acacctcata aaaaaagccc cttgtttaaa gaggctttta
    21001 tggtttttaa gtaatgttat ctatcgttta atgaccactc tcttggtaaa tggttgtccg
    21061 ttgagcgtca tttttaatac atatacacca tcaggcagtt cggaaaggtt gataattgac
    21121 ttgccattgt tttgtataaa gttgaatctt tttacctctt taccaatgac gtttatcacc
    21181 gatatttcta ttgctttctt ttcagcactg ctttcaatat tgatggctcc tgttgaaatg
    21241 gtgggataaa tatttaaaga ttcagaagag atattgttaa tgcccacagg accattagag
    21301 atggttatat cgtccagtgc tacattgaat cctaaagagt cttttatgta ttcaaatttt
    21361 acctgaatgt cggaagtgtt taaacttaat gtataaaaac taccggttag agaaaccggg
    21421 ctgtaggttt gaatatttgt ccagttagta ccatcggcag tggtatagac tttaaaagga
    21481 ttcaattgcg tgttgccatt tcctttcatg aaaaatttca ggtgtgtggc attgctgaaa
    21541 gatggtgtga taatggtagc tcccgtttga ttgaatttgt aagatgggca tgatgttgca
    21601 cacgatccgg cagtggtgta gaaagttttt cctgcggcag aggtgtcgtt caccgtaata
    21661 gtccatccgg cagggatact tgcaggcagg ccgttatatc ctgtgaaatc atacgatacc
    21721 agtacttgcg agaatacgtt ggtagtgatt acggcaaatg caattgtgag taggcgttta
    21781 atcatgtttt taaatattta tgcaaatata gctattgaaa aataataaag tgtaccaaaa
    21841 tgacgaaatc attcaatggt gttacatatt aacactttat tagggcgaat gtttggttac
    21901 attttactta gctaccatat cataatgaca atggctaaag tcagtgtttg tgctcaatgc
    21961 agacatgttg tcggtacggt aaaatgtagt gtagcctaaa gagcacaaca agttgattaa
    22021 tgctaaagcg ctgctgttgt tttctttcag attattatca tctaattcaa taaagagcaa
    22081 aggtttgtat ttttgaatag ctcctctgcc gccattgatg acagcgtatt caaatccttc
    22141 aacatcaatt tttattaaat caaggtgttc tatatttttt tctgctacaa actgatccag
    22201 cgtatcaaca tttatttttt taaaagcaat agctttctct tcgcggatta ttctgttcat
    22261 tcccggattg tgttcattga cttcatacag tttcaactcc tccttttttt caccaagacc
    22321 caggtttatg agattaatat tttggaaggt gttaagtgca atgttttcct gtgcacggat
    22381 aaaaatatcg ggatgtggtt cgaaggagaa aatttgtgca gtaccattca tggatgcata
    22441 atacaatgat gtgtagccta tgtttgcccc aacgtcaaat attactcgtg catttttcag
    22501 ttctttttgc accgattgat gtgctgcatc tgtcaatccc cagta
//
LOCUS       DAOQNP010000051        22005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_805_length_22005_cov_8.725968, whole genome shotgun sequence.
ACCESSION   DAOQNP010000051 DAOQNP010000000
VERSION     DAOQNP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22005
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_805_length_22005_cov_8.725968"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..906)
                     /locus_tag="PKY09_08105"
     CDS             complement(22..906)
                     /locus_tag="PKY09_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNB12858.1"
                     /translation="MITDKKSDAWISIVDESINADVSRIMNLFIQCSSNFFSAALIDT
                     DRNKFVALFDYRFPYPLSNDDLTEKILQIFEQNKPLLDNHYDSVVLSFCGFNNTLVPD
                     VFFDADRLDEFYYANHQRQSTETTNKLLFDNLRRISARNIYSVPKSLFDFFNSWYSNL
                     SIFHGATPFIEGVLTLHKNTSERTVTVNIQNTYFEMVVNQGGNLLYYNKFNFMSTEDF
                     IYFLVFVYEQLEMNPESQVLNIAGTTEKNSAIIVLTSKYIRNLQFVQRPSFAFYSYGF
                     DEVAAHYHFSLYNQFLCV"
     gene            complement(1009..1290)
                     /locus_tag="PKY09_08110"
     CDS             complement(1009..1290)
                     /locus_tag="PKY09_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNB12859.1"
                     /translation="MKSEIHPKNYRMVVFKDISCDYAFLTRSCVDTKDTIKWEDGNEY
                     PLVKLEISNMSHPYYTGKMKLIDTAGRVDKFRNRYAKSTAKKAAKTAEK"
     gene            1574..3451
                     /locus_tag="PKY09_08115"
     CDS             1574..3451
                     /locus_tag="PKY09_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNB12860.1"
                     /translation="MFLFERTAPRWVVLVIDLILCTLSFYLAYDLRFNFTIPDVEILL
                     LPTAFATVLSVRLVSFLISRTHTGIIRYTSYKDSQRIFLTLLSGTVLLCIFNLISFRI
                     YGRYFTPYSILMIDFFLSMLALASMRVIVKSAHVEWTRKKGNKTDVLIMGAGEEGMVV
                     KHALEQDAGINFKVLGFIDDKPKLRKRKLEGIPVFYTKTDLEDLLRANKISSLIIADK
                     DVDTKRINELIEICVKYDTKVQRAPRAGNWINGKLSFSQIKEVNIEDLLERDPINLDV
                     KEIRNQLNGKTILITGAAGSIGSEIARQVILYNPAKVVLLDIAESPLYELELSIQEIK
                     HAGNCESVIADIRNIDRMRRVFDYFKPEIVFHAAAYKHVPVMEVNPSEAILTNVKGTK
                     ILADLSVEFDVQKFVMISTDKAVNPTNVMGASKRVAEIYIQSLSEISETQFITTRFGN
                     VLGSAGSVIPRFKKQIENGMPVTVTDENITRFFMTIPEACQLVLEACKMGHGGEIFIF
                     DMGKPVKIAELAKKMIRLSGLEPEKDIRIQYTGLRPGEKLFEELLNKQENTIATHHPK
                     ILIAKVRKYKFDQIANDIESLTNLFKTQNNMALVSKMKEIVPEYISNNSEFEKLDRRE
                     I"
     gene            3633..5537
                     /locus_tag="PKY09_08120"
     CDS             3633..5537
                     /locus_tag="PKY09_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB12861.1"
                     /translation="MLVLNNISFDFGGRYLYHDAFWHIKPNQKIGLIGANGTGKSTLL
                     RIINGEYTLTGGEITGRKDLTVGFLNQDLLSYESDKSILDVALEVFERENILHQKIEK
                     ILKQLETEHGEELLNELFFLQSEFESLDGYNITHRAESILEGLGFSTADLQRPLKEFS
                     GGWRMRVMLARMLLKKPSLLMLDEPTNHLDLPSIQWLEKYLQDYDGTFILVSHDRYFL
                     DRTINMIAEVANEKINLYPGNFTNYLEQKSLRDELQQRKFENQEKYIKEQQKLIDRFK
                     AKASKAKMAQSRMKMLDRMEKVEAVSGPASTMRLSLQVETQPGKVVYEGHHLNKNYGD
                     LEIFKNSHFTIERGDKIALIGANGKGKSTLLRMIAGTEKADGEIKEGYNVKTSFYAQH
                     QLESLNIENDLMRELQIFAPLETDQRIRSILGAFLFNRDDVFKKIRVLSGGEKARVAL
                     AKLILSKANFLLLDEPTNHLDMQSVEILTDVLLDYEGSFVVVSHDRFFVEQIANKIWF
                     IENNQLKEYPGTYEEYEHWAERKKSEKRTEEKQVVVKPVVSKLLAEPDQKKQLLNLEK
                     EVKKLEAELAAAKKQRNDLMEQLGNESNYNDTAKIKMLQKEFDESEKQYNKLHSLWED
                     SYMQWMEMSE"
     gene            5545..6840
                     /locus_tag="PKY09_08125"
     CDS             5545..6840
                     /locus_tag="PKY09_08125"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNB12862.1"
                     /translation="MRQIVFYLILFFSWSDTNVFAQEKQEYFKDNYFRYDNFTYRNNI
                     KTIQLENAQFPMSEPVIELNSGAQLLLQFDDLDGDYKDYWYTIIHCDAQWKPSDITRS
                     TYLFSFYEDRIADYAFSFNTLQPYTHYTLKFPNEQIKPLISGNYLLKVYINNDPDSVA
                     FTRRLLVFENLIDVNASVHAATLAEYRQQKQEVDFSLSTTRYTIANPFGDLKVKILQN
                     FRWDNAVENLKPIFIKDNLLEYNYDEENTFNGSNEFRRFDIRSLKYETESVERIDHFD
                     TGTNVLLFPDKVRYFTRYTTDYDIDGKFEIKNYSGSHPERDADYATVRFRLEYKDEVK
                     DGNIYILGALTDWGFSNQNMLHYDEDLKAYTTTLFLKQGYYNYMYVFLKDGTSFGDES
                     WLEGNHYETENNYWILVYHRPQTERYDKLVACRKFASNR"
     gene            complement(6852..6950)
                     /gene="ffs"
                     /locus_tag="PKY09_08130"
     ncRNA           complement(6852..6950)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKY09_08130"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7026..7544
                     /locus_tag="PKY09_08135"
     CDS             7026..7544
                     /locus_tag="PKY09_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNB12863.1"
                     /translation="MFRYGLHYGGILGASSFAMFLIIYLTGNNPLGNASWLGAWIPAV
                     VIAVAVKSYRTHEGEGFISYGEGFRTGLITALLGGVLSALLIYIFCTLFDSTLVERFK
                     ADSLAQLELMEGQFKSMLSDKMYDQAVDAYNNIDLQSIAMNDFYNKLLSGIILSLIIA
                     AVYKRNKPQIPE"
     gene            7546..8529
                     /locus_tag="PKY09_08140"
     CDS             7546..8529
                     /locus_tag="PKY09_08140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB12864.1"
                     /translation="MQSSYNTDISVVIPLFNEDESLPELVTWIDRVMKQHKFSYEVIL
                     VDDGSTDKSWQVIESLSQKIKEVKGIKFKRNYGKSAALHKGFEVAQGHVVITMDADLQ
                     DSPDEIPELYEMITQQGYDLVSGWKKKRHDPISKTIPSKFFNGVTRMVSGIDNLHDFN
                     CGLKAYHHNVIKNIEIYGEMHRYIPVIAKWVGFTKIGEKVVEHRARKFGTTKFGLERF
                     VNGFLDLLSITFVSRFGKRPMHLFGTLGSLMFILGLIGACYLGVSKLYCVYNDIPATR
                     LTQRPSFYLLLTSMILGTQLFLAGFLAEMIARNDKDRNRYIVEKETANTTL"
     gene            8548..10488
                     /locus_tag="PKY09_08145"
     CDS             8548..10488
                     /locus_tag="PKY09_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB12865.1"
                     /translation="MKLSVVIVNYNVKYFLEQCLISVGKAISDIDAEVFVVDNNSVDG
                     SCQMVHKKFPFVTLIENKVNTGFSKANNQAIVQSKGEYVLLLNPDTVVEEDTFKKVVD
                     FMDSHPQAGGLGVHMIDGNGKFLPESKRGLPTPAVAFYKIFGLSALFPKSKLFGQYHL
                     GFLDKNKTNEVDVLSGAFMLLRKSVLDKIGLLDETFFMYGEDIDLSYRITKAGYKNYY
                     FPETKIIHYKGESTKKSSVNYVVVFYKAMSIFAQKHFSQSNAAFFSFLINIAIYLRAG
                     IALVNRLFSILLLPIIDAAFIYGGYSIIKTYWELYVKELHYPEHYMLLVVPGYVLVWI
                     FSAYLNGAYDKPLRISRVVRGILSGTIFILVVYALLNESYRFSRALILMNTAWACLIT
                     ALIRYALNAAGIKGFALAADVKKKLIVVADAVDAKRILSLLQLSGSNTTFVGYVESGA
                     DQGGELKDFRLGTLTQLNEIITIYQVDEVIFSSHSLSSQQIIENMGALRNRQVEYKIA
                     PPESLFIIGSNSINDNGDLYLVDVNTLNSPSNKRRKRFFDFFCAFGILICYPFLWLFF
                     KKNQNVFGNSLCVLVGKKTWVATNIKSLTATNNKVGVYTPSDGLNAPSDNEYFRDRLN
                     VLYTRDYNMITDWNIVRRNIFK"
     gene            complement(10496..11584)
                     /locus_tag="PKY09_08150"
     CDS             complement(10496..11584)
                     /locus_tag="PKY09_08150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB12866.1"
                     /translation="MIQLKSHEVINAPGRNSSVDIFRSLAILSVVIYHYDRKLPFGYL
                     GVDLFFVISGLLIGNILIGYLKKGEKINFFKFIIQRGFKIWPSYFSYLFIGSLIAVIL
                     YHTMAPDMIIPLNKYCRYIFFYSNFTSNEVWSFGHLWSLCVEEHFYIMLPILLLVIQR
                     FFPDKKVLVTALLLVIVAGFVFKVLALNFTNGKDTYGATYNRIDALGWGVLLSIILNY
                     FPTIIKQRVSQLFMLTIGVLLFCLMIFLFDNYEWFFFRKCVFHSIVPFCFFLMIAATY
                     HWNMSGLKWLRFIAYYSYNWYLWHPLWVTWFNKKIGTGLLAHLAFLIFTFLTAMAFTI
                     FIEEFFMGKRNKFIHKWFGERKIQVKAV"
     gene            complement(11590..12351)
                     /locus_tag="PKY09_08155"
     CDS             complement(11590..12351)
                     /locus_tag="PKY09_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNB12867.1"
                     /translation="MKELIRFLKSRIFWINALVIFIVIIMSIGLVYKWLDSFTDHGNS
                     VSVPDLKGMNIKKVNDFLKTKNLSFKIADSSVYLLDQKPGTIVEQDPQPDEKVKQGRT
                     IYLTITRSSAPMVKVPALKDVSLRQAEAILAASGLRMGEQIFKPDLAKNAVLSMMING
                     RDLKAGTDVPKGSAIDLIVGDGLGNTVITVPSLIGLTYDEALFVLKGSSLNIGSLFFD
                     GVIKDTLNAKIYDQNPAPDNNTINQGDAIDLYLRP"
     gene            complement(12356..15049)
                     /locus_tag="PKY09_08160"
     CDS             complement(12356..15049)
                     /locus_tag="PKY09_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNB12868.1"
                     /translation="MNKLKIGILFGGTSREREVSFAGGRTVYDNLDKTLFEPIPLFVD
                     SFGNLLILDWQFIYKGSIRDFYPPAEFLPETTRGFQIYAENLGTLTHQEQIRLTMKLG
                     TRIEFAQLPDVIDFAFLCLHGSDGEDGRIQGLLEYYRIPYSGSGILSSAIGMNKVIQK
                     DLMKKSGFNVPEVAMINRSEWVASSNRKSFIKDLKSVGFPCVVKAANQGSSIGISVLK
                     NNDADAFIQAVNKSFFIREITRTEWNSMTFDAKTEWIKKVSDIREGIGLPAKINDRII
                     YHPEELLGVLIQLFGEMEDVVSIAAIEAETEVIIESFISGKEFSCIVIANEKGKPVAL
                     PPTEIRKGNELFDYRAKYLPGLSRKVTPIHLPEVQIETIRRECERLFISLNFSVYARI
                     DGFITENGSIFLNDPNTTSGMMPSSFFFHQAAEIGLNPSQFISYIISTSLQARLSEMK
                     SFRTVPQLIKNLHGLISKKAKSQSEKIKVGVIMGGYSSERHISVESGRNVYEKLSSTE
                     KYEPIPYFLAGNEQSYSLYRIPINLMLKDNADDIREKILNYNQHPVIKKIIDQCGQVT
                     QTFANAANLNPPEKISFEELAQQVKIVFIALHGRPGEDGELQLQLNKVGLPFNGSRAE
                     SSKITIDKFQTNELLAQNGFKVAHHYKLTEEEWRNNPSAIEEKIQKEFGFPIIAKPVD
                     DGCSSAVKIIRNENQLHAYAELIFREDETLNTAAAELLKIKDKEEFPRKRYFLIETLI
                     SGENCKHFLEITGGMLTTYDETGNIHYEIFEPSETLHEGDVLSLEEKFLAGEGQNITP
                     ARYSKHAMENRLISDIIRKELMRAARLLNIEGYARIDAFVRITNDNKVEVIFIEVNSL
                     PGMTPATCIFHQAAINQYKPYEFIEKILEFGFKRNQLSLTK"
     gene            complement(15235..15999)
                     /locus_tag="PKY09_08165"
     CDS             complement(15235..15999)
                     /locus_tag="PKY09_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNB12869.1"
                     /translation="MNHHELKLYLDDLFLRYNRSEFITDDPISIPHKFTDIGDIEISA
                     FLTSIISWGQRKTILKNAQTLMEMMEHSPKSFIINASNSDYSPFKKFVHRTFNGEDCI
                     YIFKAIKNIYSEYGSLGNCFTFFVSNNPMPVAMSLFRQKLFSMPCQPRTTKHLGDPLK
                     NSGCKRFNMFLRWMIRKDCAGVDFGIWEISPSLIYCPLDVHTGRTARLLGLLKRKQDD
                     WKAVEELTQNLRQFDKTDPVKYDFALFGAGVNGLLK"
     gene            complement(16012..17331)
                     /gene="der"
                     /locus_tag="PKY09_08170"
     CDS             complement(16012..17331)
                     /gene="der"
                     /locus_tag="PKY09_08170"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNB12870.1"
                     /translation="MSNIIAIVGRPNVGKSTLFNRLTESMDAIVHDESGVTRDRHYGT
                     GEWTGKKFSVIDTGGYVSGSEDIFESEIRKQVKLAIEEADIILFVTDAREGLNDFDED
                     IAQLLRNSNKKTFLVVNKVDSPKQLNDAVEFYALGLGDYYCISSSNGSGTGELLDAVV
                     KVFKPDDESEEELPRITIIGQPNVGKSSLMNMLTGVERSIVTPIAGTTRNSINIRYSN
                     FGFDFILVDTAGLRKKAKVKEDLEFYSVMRSIRAIEQSDVCLFMIDAEKGFTAQDANI
                     FHLIQTNHKGVVFLVNKWDLIDKENNSTKKVTEEIKAAIAPFTDVPIIFTSVLNKQRI
                     FKALETAIDVFKNRTQHIPTSQLNKKILPFIENYPPPSVKGKLINIKYITQVPGPYPR
                     FVFFSSNPQYIQTTYRRFLENKLRENFNLTGVPVAISFRSKDSGKEV"
     gene            complement(17341..18216)
                     /gene="era"
                     /locus_tag="PKY09_08175"
     CDS             complement(17341..18216)
                     /gene="era"
                     /locus_tag="PKY09_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNB12871.1"
                     /translation="MHKAGFIAIVGKPNAGKSTLLNTLLGQDLSIVTHKAQTTRHRIR
                     GVVTQSDYQLVFSDTPGIITPGYQLHQRMMTFVQGAFADADVVLLVYDATGNDMDAEK
                     FKTLLPTGKKLVVALNKIDLSNQEELEKIVQKIEQQLSPAAIVPISAKEKFGGEVLIK
                     TLLQFIPEAPPYFDKDTLTDRNERFFAAEIIREKIFLQYDKEIPYSVEVSIEEFKDTP
                     KLAKIKSYIFVERESQKAILLGKNGLAIKKLGTDSRKKIEEMTGKKVFLELTVKIRED
                     WRNNEQQLNKFGYNE"
     gene            complement(18282..18908)
                     /locus_tag="PKY09_08180"
     CDS             complement(18282..18908)
                     /locus_tag="PKY09_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNB12872.1"
                     /translation="MYIPEYFRNKNFNQQVDFIKENSFGILITHSEKEMFATHIPLTS
                     LVIENHLVLYGHISKENQQCSTLINDSDALIIFSGPHCYISSSWYSHENVSTWNYQAV
                     HCQGKIKIQSEIELLQSLMQLTDHYESKEEQPKFFEKIDPKVIRDNLTGILGFEIIVS
                     NIDAKDKLSQNRNKKDYENIVTHLKKRDDAMSDAIATAMLKNKDKTVG"
     gene            complement(18926..21157)
                     /gene="purL"
                     /locus_tag="PKY09_08185"
     CDS             complement(18926..21157)
                     /gene="purL"
                     /locus_tag="PKY09_08185"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNB12873.1"
                     /translation="MESLKETSVETAKKLGLLPEEFDKICTLLGRKPNFNELSIYSVM
                     WSEHCSYKNSITWLKTLPREGPHMLAKAGEENAGLVDIGDNLACAFKIESHNHPSAIE
                     PYQGAATGVGGINRDIFTMGARPIAQLNSLRFGNLNNERTKMLMRGVVKGIGDYGNAF
                     GVPTVGGEVYFEDCYNTNILVNAMSVGIVEHGKTVKAISAGEGNSVYIVGSATGKDGI
                     HGATFASGDLHEDSHEDLPSVQVGDPFQEKLLLEATLEVIATGAVVGMQDMGAAGIIC
                     STSEMSAKGNHGMIIHLDKVPTRQKNMEPWEILLSESQERMLIVAEKGKEHLINAVFE
                     KWDLNCTCIGEVVKGEQLKFYMNETLVAEVNAESLVLGGGAPVYKRDYKEPAYFKQNK
                     LFNIDEIKVPSDLTVIAKQLASHPNICSRRWVYTQYDSMVGTINMSTNKPSDAAIVNI
                     KNSKKALAVSVDCNARYVFADPEQGCAIAVAEAARNIVCTGGEPSAVTNCLNFGNPYN
                     PEVYWQFVHSIKGMSKACEKFQTPVTGGNVSFYNQSKIGDNDVPVYPTPTIGMIGIIS
                     DKSKAATLAFSNAGDQIFLIGSSKNCIASSQYLVSTYNVNNSPAPEFNLDEEYDVQQL
                     IIQLIKTGCVQSVHDVSDGGLFISLLESAMIKGLGFEINSDEDIRKDAFLFGESQSRV
                     VVSVSEEQMDEFISIVADSGVDFTNLGVVTQGEIIVDEDKYGNISEYSKLYQEKLESE
                     MAH"
     gene            21278..>22005
                     /locus_tag="PKY09_08190"
     CDS             21278..>22005
                     /locus_tag="PKY09_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12874.1"
                     /translation="MEAVLSIGYIALFVFVIHKSAFFRIERIPVWWFKAAFIVKIFFA
                     VLLGIIYSKYYTDRLTADTFKFFDDSKVLYNLAFEHPLWFLQAISGINSEASYLLPYY
                     DIMHNWYNKVVLFNDYRFQIRINTLLRFISLGYYHVHAVFYCFLSFVGLTALLKLFVK
                     ALPELRKVLFAGVFFIPSVLFWGSGLLKDSLILFATGMALYFLNRLIQEKKPVTFFGA
                     LFFLLFPMMIKFHNFILLLPLFAA"
BASE COUNT         6835 a         3898 c         4118 g         7154 t
ORIGIN      
        1 ttccccgcaa ccgcccgctg attatacgca caaaaactga ttataaagac taaaatgata
       61 atgtgctgct acttcatcaa aaccatagct gtaaaaagca aatgaaggtc gttgcacaaa
      121 ctgcaggttg cggatatatt ttgatgtcaa tactataatt gctgaatttt tttctgttgt
      181 tcctgcaata ttcaaaacct gcgactccgg attcatttca agctgctcat aaacgaaaac
      241 aagaaaataa ataaaatctt ctgttgacat aaagttaaac ttgttgtaat aaagcaaatt
      301 tccaccctga ttgacaacca tttcaaaata ggtgttttga atattaactg ttactgttct
      361 ttcagaggtg tttttatgta aggtaagtac accttcaata aaaggcgttg cgccatgaaa
      421 aatactgagg tttgaatacc aggaattaaa gaagtcaaat agcgatttag gcacagaata
      481 gatattcctg gccgaaatac gcctgagatt atcgaacaat aatttatttg ttgtttctgt
      541 agattggcgt tgatggttgg cataataaaa ctcatctaaa cggtctgcat caaaaaacac
      601 atcaggcaca agggtgttgt taaatccgca aaaagaaagt acaacactat cgtaatgatt
      661 atctaaaaga ggtttgttct gttcaaagat ctgaagaatt ttttctgtca gatcatcatt
      721 actgagagga tatggaaaac gataatcaaa cagggctaca aacttgtttc ggtctgtatc
      781 tatcaacgct gcagaaaaaa agttgctgct gcattgaata aaaagattca ttatgcgcga
      841 tacatcggca ttgatggatt cgtcaactat acttatccag gcatctgatt ttttatcggt
      901 aatcacttta caaaattacg tatttatgaa atgcgataaa tttccgggat tatgtcaaaa
      961 aaaaatcccc tgcaatcggc agaggatctt ttctgaaaat ttctaagatt atttctcagc
     1021 tgttttagct gccttttttg cagttgattt agcataacgg ttacggaatt tatccacacg
     1081 tcctgcagtg tcaatcagtt tcatcttacc ggtatagtaa ggatgcgaca tatttgaaat
     1141 ctcaagtttt accaagggat attcgtttcc atcttcccac ttaatggtgt ctttggtatc
     1201 aacacatgaa cgcgttaaga acgcataatc gcaactgata tctttaaata ctaccatacg
     1261 gtagttcttt ggatgtattt cgcttttcat tttataataa agtttttatc aaacgggctg
     1321 caaatatagc tttttttctt attctataac aaatttgcag caaaatttat tttaagatac
     1381 tactttaatt atcagtgatt tagtgatgga atgtctgtga tgttgctgtt ttggaaatat
     1441 ggggaccaaa aaaggccggt gtttaagttt gttaataatt gtgaattatt gatattcaaa
     1501 gtatttacag cgttaaagag ctaataaaag tactattttt gcgttcaatt ttaaattttg
     1561 taaagcctga ggaatgtttc tatttgagcg tacagcacca cgttgggtag tcttagtaat
     1621 agatttgata ttatgcacct tgtcattcta tctggcgtat gatttgcgtt ttaattttac
     1681 aatacctgat gtagaaatac tactcttgcc cacagcattt gcaacagttt taagtgtcag
     1741 attggttagt tttttaatat caaggacaca tacaggtatc attcgttata caagctacaa
     1801 agattcgcag cgtatatttt taaccttact gtcgggaaca gtattgcttt gcatctttaa
     1861 tcttatttct ttcagaattt atggacgtta ttttacgcct tattctattc tgatgattga
     1921 cttttttctg agcatgcttg cattggcatc aatgcgtgtt atcgttaaaa gcgcccatgt
     1981 ggagtggaca agaaagaaag gtaataaaac cgatgtgctc attatgggtg ccggtgaaga
     2041 aggaatggtt gtgaaacatg ctttggaaca agatgcagga ataaatttta aggttcttgg
     2101 atttattgat gacaaaccaa agcttcgtaa aagaaaactt gaaggcatac cggtttttta
     2161 caccaagact gatttggaag acttacttcg agccaataaa atttcgagtc tgattattgc
     2221 cgataaagat gttgacacca aacgcatcaa tgaattaata gaaatttgtg taaagtacga
     2281 tacaaaagtt cagcgtgctc cacgtgcagg aaactggata aacggcaaac tgagcttttc
     2341 tcaaatcaag gaagtaaaca ttgaagattt acttgagcgt gatccaatta atttagatgt
     2401 aaaggaaatt cgtaatcagc taaacggaaa aactattctc atcaccggtg cagcaggttc
     2461 cattggttct gaaattgcgc gacaggtaat tttatacaat cctgcaaaag tggtgcttct
     2521 tgatattgca gagtcacctc tttatgagct tgaactttct atacaggaaa ttaaacatgc
     2581 aggtaattgc gagagtgtga ttgctgacat ccgcaacatt gaccgtatgc gtagggtgtt
     2641 tgattatttt aagcccgaaa ttgtatttca tgcagcagcc tataagcatg tgcctgtcat
     2701 ggaagtgaat ccttcagaag caattctgac taatgttaaa ggcacaaaaa ttctggcaga
     2761 cctgtctgtt gaatttgacg tgcagaagtt tgtgatgatt tcgacagata aagctgttaa
     2821 ccccacaaac gttatggggg catcaaaacg tgtagcagaa atttacattc agtcgctcag
     2881 cgaaatttcc gaaacgcaat ttatcacaac acgatttggc aatgttcttg gttcagcagg
     2941 atcagtgatt ccacgtttta aaaaacagat agaaaacggt atgcctgtta ccgtgacaga
     3001 tgaaaacatt acccgctttt ttatgactat tcctgaggct tgtcagcttg tactggaagc
     3061 ctgtaaaatg ggacatggag gcgaaatttt tattttcgat atgggcaagc ctgtaaagat
     3121 tgcagaatta gccaagaaga tgattcgcct gagtggatta gaaccggaga aggatattag
     3181 aattcaatat acaggtctga gaccgggaga aaaattgttt gaagaattat tgaataaaca
     3241 ggaaaacact attgccacac atcaccctaa aattttaatt gccaaagtac gtaagtataa
     3301 attcgatcag attgcaaatg atattgaaag tctgaccaac ctgtttaaga cgcaaaacaa
     3361 tatggcattg gtttcaaaga tgaaagaaat tgtgcctgaa tatatcagca ataacagcga
     3421 gttcgaaaag ctcgatcgca gagaaatctg agcccgttgc tgaggttctt tttcaaagag
     3481 acaaagtcat ctgattaaat ttttccggag attaattgcc agatttttac agatagatta
     3541 tataaaacat tgaatattga aagaatagct tttagagtaa taatgaatag acatttagaa
     3601 agaatagtaa aacggtttaa tatttttgca gcatgttagt tttaaataat ataagttttg
     3661 attttggagg aagatatctc tatcatgatg ctttctggca cattaagccc aatcagaaaa
     3721 taggacttat tggagcaaat ggtacaggaa aatcaacatt gctgcggatt attaatggag
     3781 aatatacttt aacgggagga gaaattacag gaagaaaaga ccttactgta ggttttctta
     3841 atcaggattt actttcatac gagtcagaca aaagcattct tgatgtggcg cttgaagttt
     3901 ttgaaagaga gaatattctt catcagaaga ttgaaaaaat tttaaaacaa ctcgaaaccg
     3961 aacatggcga agagttgctg aatgaattat tttttctgca aagcgaattt gaatcgcttg
     4021 atggttacaa catcacacac cgtgcagagt ctattttaga gggtttagga ttcagtactg
     4081 ccgatttgca acgcccattg aaagagtttt ccggaggatg gcgtatgcgt gtaatgcttg
     4141 cccgcatgct gctgaaaaaa ccaagtctgt tgatgcttga tgagccaacc aatcacctcg
     4201 atttgccttc tatacaatgg ctggagaagt atttacaaga ctatgatggt acatttattc
     4261 ttgtttcgca cgaccggtat tttcttgacc gcaccattaa catgattgca gaggttgcca
     4321 atgaaaaaat aaatctatat cctggtaact tcacaaatta tctcgaacaa aaatcgcttc
     4381 gtgatgaatt gcagcagcgt aagttcgaga atcaggaaaa gtatattaaa gagcaacaga
     4441 aactcattga ccgctttaaa gcaaaagcca gtaaggcaaa gatggctcag tccagaatga
     4501 aaatgctcga ccgtatggaa aaagttgaag cagtttccgg tccggcatca accatgcgtt
     4561 taagtttaca ggtagagaca caaccgggta aagtagttta tgaaggacat catttgaata
     4621 aaaattatgg tgatctggag atttttaaaa acagtcattt taccattgaa agaggtgata
     4681 aaattgcact cataggagcc aacggaaaag gtaagtcaac attgttgcgt atgattgccg
     4741 gtactgaaaa agcagatggc gaaattaaag aaggctacaa tgtaaagaca tcattctatg
     4801 cacaacatca gttagaaagt ttgaatattg aaaacgatct gatgcgtgaa ctgcagattt
     4861 ttgcaccttt agaaacggat cagcgtatca gaagcattct gggggcattt ctttttaacc
     4921 gtgacgatgt ttttaagaaa atacgagtgc tttcaggagg cgaaaaggca cgtgttgcat
     4981 tggcaaagct aattctcagc aaagcaaact ttctgttatt agatgaacct acaaaccacc
     5041 tcgacatgca gagtgtggaa attcttactg atgtgttgtt ggattacgaa ggctcttttg
     5101 ttgtggtatc gcacgatcgt ttttttgttg agcaaattgc caataagatt tggttcattg
     5161 aaaacaatca actcaaagaa tatccgggta cttatgaaga atatgaacat tgggcagagc
     5221 ggaaaaaatc cgaaaaacga acggaagaaa aacaagttgt ggtgaaacct gttgtatcta
     5281 aattactggc agaaccggat cagaaaaagc agttgttgaa tttagagaag gaagttaaaa
     5341 aattggaagc agagttggca gcagcaaaga aacaacgcaa tgatttaatg gaacagttag
     5401 gcaatgaaag taattataac gacacagcaa aaatcaaaat gttgcagaag gagttcgatg
     5461 aatctgaaaa acagtataac aagttacatt cattgtggga agacagctac atgcagtgga
     5521 tggaaatgag tgaataatgt gcttatgaga caaattgttt tttatctgat acttttcttt
     5581 tcatggtctg acactaatgt gtttgctcag gaaaaacaag agtattttaa ggataattat
     5641 ttcagatacg ataactttac gtacagaaat aatattaaaa ccatacaact tgaaaatgcg
     5701 caatttccga tgagtgaacc ggtcattgaa ctcaacagcg gtgcgcaact tttacttcag
     5761 tttgatgatc ttgatggtga ttataaagat tactggtaca ccatcataca ttgcgatgca
     5821 caatggaaac cttcagacat tacacgctca acttatttgt ttagcttcta cgaagaccgt
     5881 attgctgatt atgctttttc gtttaacact ttgcaacctt acacacatta tactctgaaa
     5941 tttccaaacg agcagattaa accactgata tcaggaaatt atttacttaa agtttatatt
     6001 aataatgacc ccgacagtgt agcatttaca agaaggttat tagtatttga aaatctgatt
     6061 gatgttaatg cttctgtgca tgctgcaaca ttggctgagt acaggcaaca gaagcaggaa
     6121 gttgactttt cattatctac aacaagatat actattgcca atccctttgg tgacctgaaa
     6181 gttaaaatac ttcagaattt cagatgggat aatgctgttg agaacctgaa gccgattttt
     6241 atcaaagaca atttgctgga gtataattac gatgaggaaa atacatttaa tggcagcaat
     6301 gagttcaggc gttttgatat tcgctcatta aagtacgaaa cagaaagtgt agaacgtatt
     6361 gatcattttg acaccggaac caatgtttta ctttttcccg ataaagttcg ttatttcact
     6421 cgctatacca ccgactatga cattgacgga aagtttgaaa taaaaaatta tagtggcagc
     6481 catcccgaac gtgatgccga ttatgcaacg gttcgtttcc gccttgaata taaagacgaa
     6541 gtaaaagatg gtaacatcta tattcttggt gcactaaccg attggggttt ttccaatcaa
     6601 aacatgctac attatgatga agacctgaaa gcctacacta caaccttgtt tttaaaacaa
     6661 ggctactaca attatatgta tgtgttttta aaagacggaa cctcgtttgg tgatgaaagc
     6721 tggcttgaag gcaatcatta tgaaacggaa aacaactatt ggattttagt atatcatcgt
     6781 ccgcaaacag aaagatatga taaattggtg gcctgtagaa aatttgcttc caacagatag
     6841 cctaaaataa aaatgggtaa gctacttata tcgcaccgct acaaccactt atccttgctg
     6901 cattcctgcc ctggggaggt tcgagggagc tggtcgtata agacttaccc ggtgcaaaga
     6961 tagtaaatca gacctttcaa taaacaaata aacacagcag gttttatacc tttgcatttt
     7021 aaattatgtt tcgatacgga ttacattacg gaggaatatt gggcgcatca agttttgcca
     7081 tgtttttgat aatttatctt actggaaata atccacttgg caatgcatcg tggcttggtg
     7141 catggatacc tgcagttgtc attgctgttg cagttaaaag ttatcggacg catgagggcg
     7201 aaggttttat ttcttatggc gaaggttttc gtaccggatt gataacagct cttttaggag
     7261 gtgttctttc agcattattg atttatattt tctgtacact attcgacagt acattggtag
     7321 agcgttttaa agctgattca cttgcacaac ttgaacttat ggaaggtcag tttaaatcta
     7381 tgctcagtga taaaatgtat gatcaggctg tagatgccta caataatata gacctgcagt
     7441 caatagcaat gaatgacttt tataataaac tactcagcgg aatcattcta tcacttatta
     7501 ttgctgcagt gtataaacgt aacaagcctc aaattcccga ataaaatgca gtcatcatac
     7561 aatacagata tttcagtagt tattccgttg tttaatgagg atgaatcatt accagagttg
     7621 gtaacgtgga tagaccgtgt tatgaagcag cacaagttta gctatgaagt gattttggtt
     7681 gatgatggca gcactgacaa atcctggcag gtaatagaaa gtttaagcca aaagataaaa
     7741 gaagttaaag gcataaagtt taagagaaat tatggcaagt cggctgcctt acataaaggt
     7801 ttcgaagtgg cacaaggtca tgtagtgatt actatggatg ccgatttgca ggatagcccc
     7861 gatgaaattc ctgaactgta tgaaatgatt acgcaacaag gttacgacct tgttagtggc
     7921 tggaaaaaaa agcgtcacga tccgatttca aaaaccatcc ccagtaaatt ttttaatggt
     7981 gtcacacgta tggtttccgg cattgacaac ctgcacgatt ttaattgcgg attaaaagca
     8041 tatcatcata atgtgataaa gaatattgaa atatatggtg agatgcatcg ttatattcct
     8101 gtgattgcaa agtgggttgg ctttacaaaa attggcgaaa aggttgttga acacagagct
     8161 cgaaaattcg gaactacaaa attcggatta gaaagatttg tcaatggttt tcttgatttg
     8221 ctttctatca catttgtatc acgttttggt aaacgaccca tgcatttgtt cggaacacta
     8281 ggttctctga tgtttattct tggactcata ggtgcatgtt atcttggcgt ttcaaaactt
     8341 tattgtgttt ataatgatat tcccgcaaca cgattaacac aacggccatc gttttattta
     8401 ttgctgacaa gtatgatttt aggaacgcaa ctttttcttg caggatttct ggcagaaatg
     8461 attgcacgta atgataaaga ccgcaaccgt tatattgtgg agaaggaaac agctaacaca
     8521 acattataaa ccgaaaattc tgttgcattg aagctatccg ttgtcatcgt caactacaat
     8581 gtaaagtact ttcttgagca gtgcctcatt tctgttggta aggctataag tgatattgat
     8641 gcagaagttt ttgtggttga caacaattct gttgacggat catgtcagat ggtgcacaag
     8701 aaatttccat ttgttacatt aattgagaac aaagtaaaca caggtttttc aaaggcaaac
     8761 aatcaggcta ttgttcaaag taagggtgaa tatgtgttgc tgcttaatcc tgatactgtt
     8821 gtagaggaag atacttttaa aaaggttgtt gactttatgg acagtcatcc acaggcaggc
     8881 ggacttggag tacacatgat tgatggtaat ggaaaatttt tgcctgaatc taaaagagga
     8941 cttccaacac ctgcagtggc attctataaa atcttcggac tgtcagcatt gtttccaaag
     9001 tcaaaattat ttggtcagta tcacttaggt tttctcgata aaaacaaaac caatgaagtt
     9061 gatgttctca gtggggcatt tatgttgttg cgaaaatctg ttttggataa aataggtttg
     9121 cttgacgaaa cattttttat gtatggtgaa gatattgact tgtcgtacag aataacaaag
     9181 gccggttata agaattatta tttccctgaa actaaaataa tacactacaa aggagagagt
     9241 acaaagaaaa gcagtgtaaa ttatgtagta gtattttata aagcaatgag catttttgct
     9301 cagaagcatt tttcgcagag taatgctgca tttttttcgt ttctgataaa tattgccatc
     9361 tacctcagag caggtattgc attggtgaat cgccttttta gtattttgtt gttaccaatt
     9421 attgatgcag catttattta tggtggttat tccattatta aaacatattg ggaactttat
     9481 gttaaggagc tgcattatcc tgagcattac atgctattag tagtgcccgg atatgtttta
     9541 gtctggattt tctctgctta tctgaatggt gcttatgata aaccactacg catttccaga
     9601 gttgtaagag gaattctgtc aggaacaatt tttattttgg tggtgtatgc gttgttgaac
     9661 gagtcctatc gtttttcaag agcactgatt ttgatgaata ctgcctgggc atgtttgata
     9721 acagcattga ttcgatatgc tttaaatgca gcaggtatca aaggatttgc attagcagca
     9781 gatgtgaaaa agaaattgat tgttgttgct gatgctgttg atgcaaaaag aattttgtcg
     9841 ttgctgcaat tgtcagggtc aaatacaacc tttgttggat atgttgaatc aggagctgat
     9901 cagggcggag agttgaaaga tttcagactt ggcacgttga cacagctaaa tgaaatcata
     9961 actatttatc aggttgatga agtaattttt agttcgcact cattaagttc acaacaaatt
    10021 atagagaata tgggtgcgct gcgcaacagg caagttgagt ataaaattgc accacctgaa
    10081 agcttgttta tcattggaag caattccatc aatgacaatg gtgatcttta tctggttgat
    10141 gtgaatacat taaacagccc atctaataaa agaagaaaac gattcttcga ttttttctgt
    10201 gctttcggaa tattgatttg ctatcctttt ctctggctgt ttttcaaaaa gaatcagaat
    10261 gtttttggga acagcttatg tgtacttgtt ggaaagaaaa catgggtagc aaccaatatt
    10321 aaaagtctaa ctgcaacaaa taataaagtt ggagtttata caccatccga tggattaaac
    10381 gcaccttcag ataatgaata tttccgcgac agattaaatg tgctatatac ccgtgattac
    10441 aatatgatta ccgactggaa tattgtgcgg agaaatattt ttaagtaaga aaatattata
    10501 cggctttaac ctgaattttt ctttctccaa accatttatg gataaactta tttcttttac
    10561 ccatgaaaaa ctcttctata aatatagtaa atgccattgc ggtcagaaaa gtaaaaatca
    10621 gaaaagccag atgcgcaagg agacctgttc caatcttttt gttaaaccag gttacccaaa
    10681 gcggatgcca cagataccag ttgtagctgt agtatgcaat aaatcgcagc cattttaatc
    10741 ccgacatatt ccagtgataa gtagcagcta tcatcagaaa aaagcaaaat ggaacaatag
    10801 aatgaaatac acattttcta aaaaaaaacc attcataatt atcgaataaa aaaatcatca
    10861 gacagaatag tagtactcct atagtgagca taaaaagttg tgatactcgc tgtttaatga
    10921 tagttggaaa gtaatttaat attatactca ataagacgcc ccatccaagt gcatcaattc
    10981 tgttgtaagt ggcaccataa gtatctttac cattagtaaa atttaaagca agtactttga
    11041 aaacaaatcc ggcaacaata actaatagca atgcagtaac taagactttt ttatcaggaa
    11101 agaaacgttg aataactaaa agtaagatgg gtaacatgat ataaaaatgc tcttctacac
    11161 agagtgacca taagtgccca aaactccaga cctcattact tgtgaaattt gaataaaaga
    11221 atatatagcg acagtattta ttaagtggaa taatcatatc aggagccatt gtgtgatata
    11281 gaatgactgc tattaatgaa ccgatgaaaa ggtagctgaa ataagacggc caaattttaa
    11341 accctcgttg aataataaat ttgaaaaaat ttattttctc accttttttt aaatagccaa
    11401 ttagaatatt accaattagt aatccggaga ttacaaaaaa taaatcaacg ccaagatagc
    11461 caaagggaag ttttctgtcg taatgatata taacaacact taatatagct agcgatctga
    11521 aaatatcaac acttgaattt ctacctgggg cattaattac ctcatgtgat tttagttgaa
    11581 tcatgcaagt cagggtctca gatataaatc aatggcgtca ccctgattga tagtgttgtt
    11641 atccggtgca ggattttgat catagatttt tgcattgagt gtatctttaa ttacaccatc
    11701 aaaaaataaa gaaccaatgt ttagtgatga tccttttaat acaaatagcg cttcatcata
    11761 cgttaggcct atcaaagatg gaacagtgat aacggtgttt cctaatccat cgccaacaat
    11821 caaatcaatt gccgagcctt taggtacatc tgttccggct tttaaatccc ttccgtttat
    11881 catcattgaa agtactgcat tttttgcaag atcaggtttg aaaatctgtt ctcccattct
    11941 caaccctgat gcagcaagga tagcttcagc ttgtcgtaag gaaacatctt tcaatgccgg
    12001 aactttcacc attggtgcac tgctgcgtgt tatggtgaga tagatggtac gaccttgttt
    12061 aactttttca tctggttgcg gatcttgctc aacaatagtt cccggctttt gatctaaaag
    12121 gtaaacggaa gaatcagcga ttttaaatga caggttttta gtttttaaaa aatcattaac
    12181 ctttttaata ttcatgcctt ttaaatcagg gacagagaca gagtttccat gatctgtaaa
    12241 actgtcgagc catttataaa ccaaaccaat tgacatgata attacgatga aaatcaccaa
    12301 agcattaatc cagaaaatgc gcgacttaag aaatcgaatt agttctttca taattttatt
    12361 tcgtcagcga caattgattc cttttaaaac cgaactcaag tattttctca ataaattcgt
    12421 aaggtttata ctgatttatt gcagcctgat ggaaaataca agttgcaggt gtcatacccg
    12481 gcaatgagtt tacttcaata aaaataactt caactttatt gtcatttgta atacgtacaa
    12541 atgcatcaat tcttgcataa ccctcaatat ttaacagtcg tgctgcacgc ataagttctt
    12601 ttctgataat atcagaaatc agcctgtttt ccattgcatg tttgctgtaa cgggcaggtg
    12661 tgatattttg tccttcaccg gctaaaaact tttcttcaag cgagagtaca tcaccttcat
    12721 gtaatgtttc gctgggttca aaaatttcgt aatgtatatt tcctgtctca tcataggtgg
    12781 taagcatgcc accggtaatt tctaaaaaat gtttacagtt ttcaccactg ataagcgttt
    12841 caatcagaaa atatcttttt cgtggaaatt cttctttgtc ttttatcttc agaagttcag
    12901 cggcagcagt gtttaatgtt tcatcttcgc ggaaaattag ttcggcatag gcatgtagtt
    12961 gattttcgtt acggataatt tttacagcac tactgcatcc atcatctacc ggtttggcaa
    13021 taatcgggaa tccaaattct ttttgaattt tttcttctat tgcagaagga ttattacgcc
    13081 attcttcctc tgtgagtttg taatgatgtg caactttaaa tccattttgt gccaacagct
    13141 cattagtttg aaatttatct atggtgattt tagacgattc agctcttgag ccattgaatg
    13201 gtaaaccaac tttgttaagt tgcaattgca attcaccatc ttcgcctgga cgaccatgta
    13261 aagcaatgaa aacaattttt acttgttgtg caagttcttc aaacgatatt ttttcaggtg
    13321 gatttaggtt ggctgcattg gcaaatgttt gtgttacctg accacattgg tcaattattt
    13381 ttttgattac gggatgctga ttgtaattga gaattttttc tctgatgtcg tcagcattat
    13441 ctttcagcat caggtttatt ggaattctat aaagtgaata agactgttcg ttgcctgcaa
    13501 gaaaataggg aatgggttcg tatttttctg ttgatgataa tttttcataa acattacgtc
    13561 cactttcaac gctaatgtgt ctttcactgc tataaccacc cattatgacg ccaactttta
    13621 tcttttcaga ttgtgacttg gcttttttgc tgatcagtcc atgtaggttt tttatcagtt
    13681 gtggcactgt gcgaaacgat ttcatttctg aaaggcgtgc ctgaagcgat gttgaaataa
    13741 tgtagctgat aaactgtgaa ggattgagac caatttctgc agcctgatga aagaaaaagc
    13801 tgctgggcat cattcctgat gttgtattcg gatcattcaa aaatattgaa ccattctctg
    13861 tgataaatcc atctatgcgg gcataaacac taaagtttaa tgatataaac aagcgttcac
    13921 attctcttct tattgtttca atttgtactt caggcaggtg aataggtgtt acttttcgtg
    13981 ataaaccggg cagatattta gcacggtaat caaacaactc attgccttta cggatttctg
    14041 ttggcggtag cgcaacaggc tttccttttt catttgcaat tacaatacat gaaaactctt
    14101 ttcctgaaat gaacgattca atgataactt ctgtttcagc ttcaatagca gcaatggaaa
    14161 caacgtcttc catttctcca aaaagttgaa tcagtacacc taatagttct tccggatgat
    14221 aaattattct gtcgttaatt tttgctggta atccaatgcc ttcccgaatg tctgatactt
    14281 tcttaatcca ttcagttttt gcatcaaaag tcatgctgtt ccattctgtt cgcgtgattt
    14341 cgcggataaa aaatgattta ttgacagctt gtataaatgc atctgcatca ttattcttta
    14401 acactgatat gccaatgctc gaaccctgat tggcagcttt aacaacacat ggaaatccaa
    14461 cagattttaa atctttaata aaagattttc ggtttgaaga ggctacccat tcagagcggt
    14521 ttatcatagc aacttccgga acattaaagc ctgatttttt catcagatct ttctgaataa
    14581 ccttgttcat tccaattgca gaagaaagaa tacctgagcc cgagtaggga atccggtaat
    14641 attccaaaag accttgtatg cgtccatcct caccatcaga accatgcaga cataagaatg
    14701 caaaatcaat aacatcaggc aactgtgcaa attctatacg cgttcctaat ttcattgtca
    14761 gcctgatttg ttcctgatgt gtaagtgtgc caagattttc tgcgtaaatc tgaaagcctc
    14821 gtgttgtctc aggcaaaaat tctgcgggcg gataaaaatc acgaatacta cccttgtaaa
    14881 taaattgcca gtcgagaatc aacaggttac caaaactgtc aacaaaaaga ggaataggtt
    14941 caaaaagcgt tttgtccagg ttgtcatata cggttctgcc tccggcaaag gatacttcac
    15001 gttcgcgtga tgtgccgcca aagagaattc ctatttttag tttattcata tagattttaa
    15061 gtcatgtaat gattgaacaa agttaataga tttcagcagc agcatactta cttcaagagt
    15121 aagtattcac gtttttttta acatttatta tcagaggatt atttgataaa tccatgatag
    15181 aaacattctt ttgttaattg gtttttacca agtaaaacga ctgaaaacga ctggttattt
    15241 taacaatccg ttgacgcctg cgccaaaaag agcaaagtcg tattttacag gatctgtttt
    15301 atcaaactgt cgcagatttt gggtcagttc ttcaacggct ttccagtcat cttgctttct
    15361 ttttagcagc ccaagtagtc gtgcagttcg accggtatgt acatctaaag gacagtaaat
    15421 caacgatggt gagatttccc aaattccaaa gtcaacaccg gcacaatctt tcctaatcat
    15481 ccaacgcaga aacatgttga atcttttaca tcctgagttt tttaatgggt cacctaaatg
    15541 ttttgtggtg cgtggctggc atggcattga gaacagtttc tgtctgaata aactcatggc
    15601 cacaggcatg ggattgttgc ttacaaaaaa ggtaaaacaa ttaccaagcg aaccatattc
    15661 agagtaaata tttttaatcg ctttaaaaat gtagatacag tcttctccat taaaagtgcg
    15721 gtgaacaaat tttttaaaag ggctataatc gctgtttgat gcatttataa taaatgattt
    15781 tggagaatgt tccatcattt ccataagtgt ttgtgcgttt ttgagaatgg ttttgcgctg
    15841 cccccatgag ataattgagg tcagaaaggc actaatttca atatcaccaa tgtcagtaaa
    15901 cttatgcggg atagagattg gatcatcagt aataaattca cttctgttgt accgaagaaa
    15961 taaatcgtct aaatacagtt ttagctcatg atgattcatg acttgctgtt atcagacttc
    16021 ttttccactg tctttgcttc taaaagaaat tgcaacagga acgccagtca gattaaaatt
    16081 ttcacgcagt ttattttcaa gaaatcttct gtatgttgtc tgaatgtact gtggattact
    16141 actaaaaaaa acaaaacgtg gatagggtcc gggcacctga gtaatatatt tgatgttgat
    16201 gagtttgcct tttacagatg gcggaggata gttttctatg aaaggtagaa tttttttgtt
    16261 aagttgtgat gtcggaatgt gttgtgttct gtttttaaaa acatcaatgg cagtttcaag
    16321 tgctttaaaa atacgttgct tgtttagcac agaagtaaag attatgggca catcggtaaa
    16381 aggcgcaatg gctgctttaa tctcttctgt aactttcttt gttgaattgt tttctttatc
    16441 aattaaatcc catttattca ccaagaagac aacacccttg tgattggttt gaatgaggtg
    16501 aaaaatattt gcatcctgag ctgtaaatcc tttttctgca tcaatcataa acagacacac
    16561 atcgctttgt tcaatggctc ttatgctgcg catgacagag tagaactcca gatcttcttt
    16621 cactttggct ttctttctca atccggctgt atcaactaaa ataaaatcga aaccaaaatt
    16681 tgagtaacgg atattaatgc tgtttctggt agttcctgca ataggggtaa caatacttcg
    16741 ttccacaccg gtaagcatat tcatcaatga tgatttaccg acattaggtt gtccgattat
    16801 agtaattcgc ggaagttctt cttcagattc atcatcgggc ttaaaaactt ttacaactgc
    16861 atcaagcagt tcgccagtac cgctaccgtt agaagacgat atacaataat aatcaccaag
    16921 gcctaatgca taaaattcaa cagcatcatt cagttgtttt ggagaatcaa ctttgttcac
    16981 aactaaaaat gttttcttat tgctgttacg aagcagttgt gcaatatcct catcaaaatc
    17041 attcagtcct tcgcgggcat ccgtaacaaa aaggataatg tctgcctctt cgattgccag
    17101 tttaacttgc tttcgaattt cagattcaaa aatatcttct gatccggata cgtaacctcc
    17161 tgtatcaatg acagaaaatt tcttcccggt ccattctcct gttccgtagt gcctgtctcg
    17221 tgttacacct gattcgtcat gtacgatggc atccatactc tctgtcaacc tgttgaacag
    17281 tgttgatttt cctacattgg gtctgcctac aatagcaata atgttgctca tttttctttg
    17341 ttattcgttg tagccgaatt tgttcagctg ttgctcgtta ttacgccagt cttctcttat
    17401 cttaactgta agttcaagaa atactttttt gcccgtcatt tcttcaattt tctttctgct
    17461 gtcagttcct aattttttta ttgccaaacc attttttccc aataaaatgg ctttctgtga
    17521 ttctctttca acaaaaatat aactctttat ttttgccagt ttaggcgtgt ctttaaattc
    17581 ttctatactt acttcaacac tataaggtat ttctttatca tattgcagaa aaattttctc
    17641 gcggataatt tcggcagcaa aaaacctttc attcctgtca gtcagtgtgt ccttgtcaaa
    17701 ataaggaggt gcttcgggga taaattgtag caaggtttta atgagaacct cgccaccaaa
    17761 tttttcttta gccgaaatag gtactatggc agcaggacta agctgttgct caattttctg
    17821 aacgattttt tcaagttctt cctgattact taaatcaatt ttattcaatg ctacaactaa
    17881 cttttttccg gtaggaagca gtgttttgaa tttttctgca tccatatcat ttccggtagc
    17941 gtcatatacc agcagaacga catcagcatc tgcgaatgca ccttgaacaa acgtcatcat
    18001 tcgttgatga agctgatatc cgggggtgat aatgcccgga gtgtctgaaa acaccaattg
    18061 ataatcagac tgtgttacta cacctcttat tctgtgacgt gtagtttggg ccttatgggt
    18121 cacaatactc aagtcctgcc ccaaaagcgt gtttaacagg gtagattttc ctgcattggg
    18181 tttgcctact attgctatga atcctgcttt atgcactttc aaattttcct gcgaaggtag
    18241 tcaagaaaag gggacaaaat ttactttgat gaaattagat gttacccgac cgttttatcc
    18301 ttgtttttaa gcatagccgt tgctatggca tcagacatgg catcatcacg ctttttgaga
    18361 tgggtaacaa tattttcgta atcttttttg tttcggtttt gagaaagttt gtcttttgca
    18421 tcaatgtttg aaacaataat ttcaaatcca agtattccgg ttaaattgtc acgaataact
    18481 ttagggtcta tcttttcaaa aaatttaggt tgttcttctt tcgattcgta atgatcggtt
    18541 agctgcatca aactttgtaa cagctcaatc tctgattgta ttttaatttt accttgacaa
    18601 tgtacagcct gataattcca ggttgaaaca ttttcgtgac tataccaaga ggaagaaata
    18661 tagcaatgcg gaccactaaa aattatcaac gcatcactgt catttattaa tgtgctgcat
    18721 tgttgatttt cttttgaaat atgtccatat aataccagat ggttctcaat tactaaagac
    18781 gtcagcggaa tgtgcgtagc aaacatttct ttttctgaat gtgtaatcaa aattccgaaa
    18841 ctgttttctt taataaagtc aacctgttgg ttaaaattct tatttcgaaa atattcaggg
    18901 atgtacataa gtagaacaaa tgcttttagt gagccatctc actttcaagt ttttcctgat
    18961 agagctttga atattcgctg atgttaccat atttatcttc atcaacaatt atttcgccct
    19021 gtgtcacaac gcccagattg gtaaaatcaa caccggagtc agctactatg ctgataaatt
    19081 catccatttg ctcttcagaa acacttacta caacacgact ctgagattct ccaaacagaa
    19141 atgcatcttt tctaatatcc tcatcagaat tgatttcaaa acctaggcct tttatcattg
    19201 cagactcaag tagtgaaata aaaagaccac catcagaaac atcatgtacg gactgaacac
    19261 atccggtttt gattaactgt atgatgagtt gctgaacatc atactcttca tcaagattaa
    19321 attcaggcgc aggactgttg ttgacattat atgtgctaac aagatattgc gatgaagcaa
    19381 tgcagttttt tgaagagcca atcagaaata tctgatcacc tgcatttgaa aatgccaatg
    19441 ttgcggcttt gcttttgtca ctgataatgc caatcatacc aattgttgga gtagggtaaa
    19501 ccggcacatc gttatctccg attttagact gattgtaaaa acttacatta ccacctgtca
    19561 ccggagtttg aaacttttca cacgcttttg acataccttt aatactatgt acaaactgcc
    19621 agtaaacttc agggttatac ggattgccaa agttcagaca attcgtaaca gcagaaggtt
    19681 ctcctcctgt acaaacaata tttcgtgcag cctctgctac tgcaattgca caaccttgtt
    19741 cagggtctgc aaacacatat cttgcattgc agtcaacaga cacagcaagg gccttttttg
    19801 aatttttaat atttacgata gcagcatcag atggtttgtt ggtactcata ttaattgtac
    19861 ccaccatact atcatattgt gtgtaaaccc agcgtctgct gcaaatattc ggatgcgatg
    19921 ctaattgttt agcaataaca gtaagatcag atggaacttt tatttcatct atgttaaata
    19981 atttgttttg tttgaagtac gcaggttctt tataatctct tttataaacc ggtgcaccac
    20041 cacccaatac taatgattca gcattaacct cagcaaccaa cgtttcattc atatagaatt
    20101 tcaattgctc acctttaact acttcgccaa tgcatgtaca gtttaagtcc catttttcaa
    20161 aaacagcatt aattaaatgc tctttacctt tttcagcaac aataagcatt ctctcctgcg
    20221 actcagaaag taaaatttcc catggctcca tgtttttttg tcttgtagga actttatcaa
    20281 gatgtataat catgccatgg tttcctttgg ccgacatttc ggaggtagaa caaataattc
    20341 ctgcagcacc catatcctgc attcctacaa cagcacctgt tgcaatgact tcaagtgttg
    20401 cctctaataa aagtttttcc tgaaatggat ctccaacctg cacagagggt aaatcctcat
    20461 gcgaatcttc atgcaagtcg cccgatgcaa aagtggcacc gtgaataccg tctttccctg
    20521 ttgctgagcc tacaatataa acactgttac cttcacctgc tgatattgct tttacagttt
    20581 tgccatgttc aacaattccc acgctcatgg cattaactaa aatgtttgta ttgtagcaat
    20641 cttcaaaata aacttcgcca ccaacagttg gaacaccaaa ggcatttcca tagtcgccaa
    20701 ttcctttaac aacaccacgc atgagcattt ttgtgcgttc attattcagg ttgccaaagc
    20761 gcaaagagtt aagttgtgca ataggtcttg cccccatagt aaaaatatcg cggttaattc
    20821 cgccaacgcc tgttgctgct ccctgatagg gttctatggc agatgggtga ttgtgcgatt
    20881 caattttaaa agcacaggca agattgtcgc caatatcaac aagacctgca ttttcttctc
    20941 ctgctttggc aagcatatgt ggcccttcac gaggcaatgt tttcagccat gtaatggagt
    21001 ttttatagga gcaatgttca ctccacatca cagagtagat actcaattca ttaaaattag
    21061 gtttgcgacc taataacgta cagattttat caaactcttc aggtaaaaga cctaattttt
    21121 ttgctgtttc aacagatgtt tctttcaaac tttccattct ttatttttaa agtataaatt
    21181 cgtttcagaa tacttttatg ttactttgcg caaaggtaac ctattgatgt aataaacagg
    21241 ttattgtatt atatgaaaat tttcaaacag ccttactttg gaagcagttc ttagcattgg
    21301 gtatatagct ttatttgttt ttgtcattca taaatctgca tttttcagga ttgaaagaat
    21361 tcctgtatgg tggtttaaag cagcttttat tgttaaaata ttttttgccg ttttacttgg
    21421 aataatttat tctaaatact ataccgacag gttaactgcc gatactttta agttttttga
    21481 tgatagtaag gtgttgtaca atctggcttt tgaacatccg ctttggtttt tgcaggcaat
    21541 ttcaggaata aatagtgagg caagctattt actcccttac tatgatataa tgcataactg
    21601 gtacaataag gtagtcttgt ttaacgacta tcgttttcag attaggatta atacattgtt
    21661 gcgttttata agtttaggct actatcatgt gcatgctgta ttttattgtt tcttgtcatt
    21721 tgttggactc actgcactgc tcaaactttt tgttaaagca cttccagagc tcaggaaagt
    21781 tctgtttgcc ggagtgtttt ttattccttc agttttgttt tggggatcag gattgttgaa
    21841 agattcactg attctttttg caacaggcat ggcactttac tttttgaatc gcttgattca
    21901 ggagaaaaaa ccggtaacat tttttggtgc tttatttttt ttactttttc cgatgatgat
    21961 taaatttcat aattttattt tgctgttgcc actctttgca gctta
//
LOCUS       DAOQNP010000052        22005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_806_length_22005_cov_8.430387, whole genome shotgun sequence.
ACCESSION   DAOQNP010000052 DAOQNP010000000
VERSION     DAOQNP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 22005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22005
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_806_length_22005_cov_8.430387"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PKY09_08195"
     CDS             <1..1181
                     /locus_tag="PKY09_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNB12875.1"
                     /translation="AGVFYFVLVMLGMALSIGSQILMSRKAGENNRLAIGNYFDHSVV
                     ILSVLAVILYFIMFFAAPAFFNLVLHSKDVADASITYIHNRSFGIFFILIVNCFRAFY
                     TSISATRFITYSALVLTVSNFILDYVLIFGKLGFEAQGIAGAARASAIAEVLACIYLF
                     FITFYRNENSSFGLFRFVNINLTRINEIIFISIPVFFQNLLSMGAWFLFFVFIERLGQ
                     HELAVSNVVRSAYMVLMTPIWGYASAANSMVSNLIGQNKQDEVIPLVKKIIVLSLASS
                     TLILLINLFDFKLLLRIISSDMMLLYDATPSYIVTWCAMFFFSVSMVLLSAISGTGKT
                     NVAFIIEVINIAIYVAYAFITAVLLKAQLHVVWYVEILYWIIMGLCSWLYLQTNKWRK
                     "
     gene            1178..1474
                     /locus_tag="PKY09_08200"
     CDS             1178..1474
                     /locus_tag="PKY09_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12876.1"
                     /translation="MKHVLLILFIYMLASCKSAKQETIHQNLETVTVTDMRNLDGCGF
                     LLKTTDQKMLIPDNLDDSFQKNNLKLEISYKKTSVMTTCMAGQTITLLYCRKAK"
     gene            1489..2445
                     /gene="fmt"
                     /locus_tag="PKY09_08205"
     CDS             1489..2445
                     /gene="fmt"
                     /locus_tag="PKY09_08205"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNB12877.1"
                     /translation="MNDFKILFSGTPDFAVESLKILVENGYNICGVITAPDKPAGRGM
                     QMQQSAVKKYALSKGLYILQPEKLKDAVFLSEVASLKADLHIVVAFRMMPEQLWSMPR
                     LGTLNLHASLLPQYRGAAPINRAIMNGETTTGVTTFFLKHEIDTGDIILSDKVDIGPT
                     ETAGELHDRLMHIGAALVLKTVQQIEKGNLSTTPQKSITSELKHAPKIFKNDCIINWS
                     DKTSTVFNQIRGLSPYPGAFTMLEDKNGKTLTLKIYSALFLESKELSETGKIISDNKS
                     FLNITTSDGIISIKELQLEGKKRMSAEEFLRGFSINDYTVKK"
     gene            2499..3188
                     /locus_tag="PKY09_08210"
     CDS             2499..3188
                     /locus_tag="PKY09_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNB12878.1"
                     /translation="MKRIVVLTGAGISAESGIKTFRDSNGLWEEHRIEDVATPQAWQR
                     NPKLVTNFYNQRRKQVMEALPNKAHLALVELEKHYQVVVITQNVDDLHERAGSKHIIH
                     LHGEILKMRSEEDPSMIYQSNGWEMDYESTNDHGHRLRPHIVWFGEMVPKMDEAIYQT
                     MKADIFLVIGTSLEVYPAAGLIDYTSDSCEKYIVDPKARELNHIQNLTVIQNTAGEGV
                     PALVNKLIKNV"
     gene            3181..3786
                     /locus_tag="PKY09_08215"
     CDS             3181..3786
                     /locus_tag="PKY09_08215"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNB12879.1"
                     /translation="MFSEAFMQTDLYSWVVLPVLIFLCRMCDVTLATLRNIFLSRGVR
                     KIIPIIGFVEVLIWLLAVSSIIKNLHNIMCYVAFAGGYSMGILVGISIEKRLALGTQI
                     IRVITSRQSEKLLQALRAQNLGVTEIDAMGSQGPVKILLIVAKRKNIASILEIVNEHH
                     GSSFFTIEDVRSVEQGIFPIKAGEGKIQYLRRIFPTFKPGR"
     gene            3851..4474
                     /locus_tag="PKY09_08220"
     CDS             3851..4474
                     /locus_tag="PKY09_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12880.1"
                     /translation="MSTNKTDSKERSIRVSRIERIHPYRTILFFGILISSVLFLTLSF
                     LFLITQNENSNLPPALPKVFTLSTILLLFGSYTISKAVSAFRKDSFDKLLRATILTTI
                     ITSLFLVTQIIGLHAMQKTGYYTNMPFNSHYFFGIIALHMVHALATVAYLIFITYNAF
                     HQSHDVIDSLLFFSDQGQLLKLNSAMVFSHFVNFSWLAIFLMFLFSF"
     gene            4474..5442
                     /locus_tag="PKY09_08225"
     CDS             4474..5442
                     /locus_tag="PKY09_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12881.1"
                     /translation="MISKSHAVTLALLLTLFFSCKKENLDLVFEEINTPANFNLNNLF
                     ILNDSVVFACGGQTGNGIILRSSDGGKSWHILNDQFEYNIHSIYFLDNQLGYCGGDST
                     HVYQTKDGGISWQLHFDWEGIPFQYSSPLRNVCFLNKDTGFFAGGKNFDRGIIYSTAD
                     GGEHWNVSGFEHELRCITPTAAGILSGGYGIMLLSKQNFEFASLDCDRSFYTGIAFSN
                     LLEGVACSYNGALYHTSDGGNTFSIKDKKNHTFGKNEQFLCIDARKEKIIASGLSGFT
                     AVSTDGGENFDSGYSLNNSRINSIKFLNDTIAIAISDNGKVFRIHL"
     gene            complement(5732..6472)
                     /locus_tag="PKY09_08230"
     CDS             complement(5732..6472)
                     /locus_tag="PKY09_08230"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNB12882.1"
                     /translation="MFKNSIWYNLHETESTNSFLHEKLMSEKLPEGSVVTADYQTKGR
                     GQRGSSWQSEAGKNLLMSIVVYPEFLKVTEAFMLSKCVALAACDLLSVYSNKVQIKWP
                     NDILIDGKKVAGILIENILRGSEIYASIVGIGINFNQQLFEKELYAATSVFMDTGFKA
                     DTQINAVKLHENIEVNYQMLQQKKFAEIDQLYLSHLYKLNETALFKDADGQFQGKIKG
                     LSSMGLLIVERENGEKHLYDVKEIALIR"
     gene            6544..7002
                     /gene="rsfS"
                     /locus_tag="PKY09_08235"
     CDS             6544..7002
                     /gene="rsfS"
                     /locus_tag="PKY09_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNB12883.1"
                     /translation="MAKRTNPAKDKDETTPVKAATLKKTKARQPKRKLKDNTDKLLTV
                     IIDSIEDKKGERIVVLNLKELSNAMADYFIICQASNKTQVEAIVRNIEEKVEKKLKET
                     AAHIEGKQNAEWILMDYFNVIVHVFLADKRDFYSLEKLWADAPLVKFAAQ"
     gene            7125..9140
                     /gene="ftsH"
                     /locus_tag="PKY09_08240"
     CDS             7125..9140
                     /gene="ftsH"
                     /locus_tag="PKY09_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNB12884.1"
                     /translation="MPKPPGKGFNFSWIYAIVFLLIFVIWVLGLGDKPKLTNMNEFEK
                     TMLATHDVEKIEIVNRERVEITIKKDKLNQEKYKAVSHKPFGNGYNPGPHYYMPIGDV
                     GNFESKLEAIQKDFPENERIQPEYNNNDRSILEVLFWNLLPFIIIAVIWIFIMRRMGG
                     GGAGSQIFNIGKSKAQLFDKDLNVKVTFNDVAGLEEAKVEVMEIVDFLKNPKKYTQLG
                     GKIPRGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDLFRQ
                     AKEKAPSIIFIDEIDAIGRARGKAPSFSANDERESTLNQLLTEMDGFGSNTGVIILAA
                     TNRADILDRALLRPGRFDRQIYVELPDLTGREEIFKVHTKALKIDHTVDIKFLARQTP
                     GFSGADIANLCNEAALIAARKNKTAIGKDDFLSAVDRIIGGLEKKNKIITKHEKSVIA
                     YHESGHATVSWLLEHAHPLVKVTIVPRGNSLGAAWYLPEERQLTNKEQIFDEICAALG
                     GRAAEEVIFGTISTGALSDLEKITKQAYASVSIFGLNERIGNISYYDSSGQQDYSFSK
                     PYSEKTAEIIDEEVKKLIDLAYQRTKKLLVDNKEKLDKLAHRLLEKEVIFKEDLEEIF
                     GHRPWGESEKATFNSFITDGEPEPEKTIPPAVDSPPPSNIPQGSIQV"
     gene            9144..9797
                     /locus_tag="PKY09_08245"
     CDS             9144..9797
                     /locus_tag="PKY09_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12885.1"
                     /translation="MDESTNREKVLKKVRSALIAKTPNPYPNLDMDKGVYNSMSYSPE
                     VQFAENFTQQGGQFVLCNGRVELMETILGVCDANDFTEVVCVDRNLSAFFDEFEFPHQ
                     SVFSEDDDTDIQAVIIPCECLVVRNGLIVLSEDNIYQKALLRVTQHLVIIAHTQQLIP
                     DMTEALAYVRYQNDSNVPSELYFATPNFLNQIQNAVAANASVKSMTVILIQPYDSEL"
     gene            9991..11796
                     /locus_tag="PKY09_08250"
     CDS             9991..11796
                     /locus_tag="PKY09_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12886.1"
                     /translation="MSHSDLHRFHIPVMGLAFTVDTPVKVARYGISSVVSIIEHNMLE
                     VMREFYYKQTGKEFTAITKKEEDYRAKRVTAYLNIINEIVLKQITDLRNESFETGKEI
                     NKYFEMLPDTSEAKLLYRKMQASANQVEKEQMQQHLRTLIKSGAIDVNIMTKVDRINY
                     DKNEQPLAQEFSDAHNSLRGFAHSNLNSSIVFSAGLNPRLYSYCEKFDDFFPDESGFV
                     KKKITLKVSDFRSAQVQGRFFAKKGIWISEFRVESGLNCGGHAFATDALLLGPILEEF
                     KLNKNALYDELFNTCQAALAQKNKPQYKEKPKMKVTVQGGIGTSQENDFLYHHYEVDL
                     TGWGSPFLLVPEATNVDKETLGLLATASKDDYYLSEASPLGVPFNNFRKSSAERQRKE
                     RIAKNRPGSACYKKFLAFNTEYGGEPICTASRKYQHLKLQELKQANVEPSQKQIDDLL
                     IKDCLCEGLASPVMLVNNVLVPHNLNAVTICPGPNLAYFSGTFTLQEMINHIYGRANV
                     LNTLYRPHMFINELNLYIEYLKKDIQKSAAKINDKKEKQLQLFKENLSKGIEYYRNLS
                     NKLSNFTKTNIENFQKDLQECENILKSMLVEEVQY"
     gene            11986..12975
                     /locus_tag="PKY09_08255"
     CDS             11986..12975
                     /locus_tag="PKY09_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB12887.1"
                     /translation="MTCGKFVVSLDFELLWGVRDKRTIQSYGENLRGVHQAIPRLLSL
                     FDKYQIKGTFATVGFLFFETKEELLANIPKTIPTYTNTELSPYQGHFNLLGENYIEDV
                     YHFAPQLINEIKKYPEQEISTHTFSHYYCLENGQTKENFKADIEAALHIAEKNGIVIT
                     SLVFPRNQFNEVYINIIKELGLNSYRGNENSWLYKAKKGEQESSFRRALRLIDAYFNI
                     SGHNCYSDDFLRSKYPMDIPSSRFLRPYSKLLSALEGFRLKRILSGMTFAAKHNQTYH
                     LWWHPHNFGVNQNENFSFLEKILEHYKHLNIKYGFKSITMSQLSTELNKKRHE"
     gene            12968..13903
                     /locus_tag="PKY09_08260"
     CDS             12968..13903
                     /locus_tag="PKY09_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12888.1"
                     /translation="MSELVKFSKITLQDFPIIQKIALENGGSKTINESNFNHWYLKNP
                     SASNSIFKVEVENSIEGYATTNNFVYTVENKNYLVALPQNVLTTEKCRGKGLFAKLYY
                     LTEKENLETNKVDFFLTSTNTMSTPIFLSKFGYLRGFCPPILLKLFSPLSLFSKANYK
                     QQEDINAIKIDHNDLNNSRKKNLAYFKWRYSQTNKKNLTVISIIENNQIIGYAFLIKQ
                     IKRGFKVLMLADIICSNEKFYSKIIKACHTYASKNFYVGLLMFELQNNNINQGRSIKL
                     KNKFNFLVKGKSDDETKKLSQVKFNYFFGDLDYFW"
     gene            complement(14011..15114)
                     /locus_tag="PKY09_08265"
     CDS             complement(14011..15114)
                     /locus_tag="PKY09_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12889.1"
                     /translation="MKQLYTTMIALASVFCAHAQITLTQSDFAGAGDNILVSNADATM
                     PIDFTTTGANQTWDFSTLIAQSQDTLAFLSVSSTGGTYSVYFANVGFNSNRSNIAIPF
                     GNLPAIPGLPITLSDPYSFFYKSSGDFKQQGLGITISGFQTPIAFSSKDVLYNFPIAF
                     NDVDSCQAAWSFSLSGLGYYGFEQKRVNHVDGWGTINTPYGSFNALRIKTELYAHDTL
                     YADTLGFGYSMDRPKAVEYKWFTNNEKIPLLQINTTDVLGTETVASVVYIDSLRSVGI
                     ASTELLSNFSVAPNPAEKYFVINYSLMKPGQVLIKLTDMKGVEVMNETQGVQSTGSHQ
                     KMIGTQLLSAGVYFLTITTDNFSQTQKVMIRKN"
     gene            complement(15136..16299)
                     /locus_tag="PKY09_08270"
     CDS             complement(15136..16299)
                     /locus_tag="PKY09_08270"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNB12890.1"
                     /translation="MPQYYSQGKIPHKRHTQFRKPDGGLYAEELFSTHGFSNMYSLIY
                     HCHPPTMIKSAGEPFSREPEIAMKRSLLHQSFQGFNVQPEDDYIKSKKPVLVNNDLQI
                     ALAAPRKSTKDYFLKNADADELIFVHEGSGTFHSIYGSMDFGYGDYIVIPRGTIFQLH
                     FNTENNRLLVVDSFSAIQTPRRYRNEYGQFEEHSPFCERDFRRPQNLKTFDEKGEFLV
                     MIKKESIVYPFVYATHPFDAIGWDGYCYPYIFSIHDFEPITGRIHLPPPIHQTFEAHN
                     FVVCSFVPRLFDYHPLSIPVPYNHSNIDSDEMLYYVDGDFMSRKNVTKGQITLHPAGI
                     PHGPHPGTVEKSLGAKETKELAVMIDTFKPLWLTKEAVSIMIPDYYKSWVENG"
     gene            16484..17461
                     /locus_tag="PKY09_08275"
     CDS             16484..17461
                     /locus_tag="PKY09_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNB12891.1"
                     /translation="MNMLGKSIAVVLISTLAFSQDVMAQRMKTEDYISRYKEDAIKDM
                     IKTGVPACITLAQGILESESGNSKLAIEANNHFGIKCHKEWQGKQFHQDDDAENECFR
                     KYNNALESFDDHSDFLRSRPRYASLFELKPTDYKGWAHGLKKAGYATNPNYAHKLIDL
                     IERFNLEDINNEAIARMNGKPIPEQPTLARNVNLPKGSPSAKEVEKTTVINITPPSYY
                     SEINNVKYIVSKKGDTWLSLAKQNEMMLWQMLKYNDASKDDVLKEGTIVYIKPKRNSA
                     KQDYHIVKQGETMRDISQLYAIKLNKLYKKNQLEPGAALEPGMRIKLNN"
     gene            17522..19222
                     /locus_tag="PKY09_08280"
     CDS             17522..19222
                     /locus_tag="PKY09_08280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNB12892.1"
                     /translation="MKVVIFLNCIFTLIISNAYSQQTVIPLSPLTIPLVQKGNYGEVR
                     SNHFHSGLDFSTQTKIGFPVIASFDGYVSRIKTSENGYGKAVYIDHSNGLSTVYAHLH
                     RYGKTIEPFVFTEQEKQKNYSVELFPERNKIMIRQGDTIGWSGNSGGSSGPHLHYEIR
                     NTKSEKPLNPISYFPQIKDTIPPLIKSITIYSIKENFLSIFPWHEIKPMETSRGYYSY
                     TELVNISGEIGIGFTGYDQANNDNAILGIDEVELYANEKLVFARKMSDFSFEETRNAN
                     GLIDYSKGQRTGNITERCYKLPGVKFSQYVFLKNSGVVDVENDSTINFRLMLRDGSGN
                     ISRCVFTVVNNSNAKPKNTVVKKGELLEYNKANKIQSDRYKLLLPEGALFRDFYCDAI
                     IRRGIKSFIAPQITIGSTSEGLKRNVTLKIKSDLEGDELNNKTIMVKIASNGTTSPVS
                     GIYKDGWYSSSISSFGTYSLMIDSIAPVIENITTSYDSVYNCPVLEVKVSDNFSGIKN
                     YSCTINNKWYLAEYDRKNSTIYVLLNRFTENEFNFKIDLTDVCGNTTIFCKEIILIPK
                     "
     gene            complement(19227..20102)
                     /locus_tag="PKY09_08285"
     CDS             complement(19227..20102)
                     /locus_tag="PKY09_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB12893.1"
                     /translation="MQLQTIDTGFFKLDGGAMFGVVPKSIWQKLNPADDNNMCTWALR
                     CLLVEDGNRLILIDNSMGDKQSQKFFSHYYLHGDDTLEKSLSKAGYHSDDITDIFLTH
                     LHFDHCGGSIKWNSARDGYLPAFKNATYWCHADHWKWATEPNAREKASFLKENILPIK
                     ESGQLKFFEGTSDMSNGFEIISKNNFHSNIDLVVARGHTDGMTLPLIPYKNGKLIFMA
                     DLLPSAAHVPLPYVMAYDTRPLVTLEEKKAFLKYAVNNNLNLFLEHDPVSECCNLMET
                     EKGVAVSSTYKLSEI"
     gene            complement(20083..21369)
                     /locus_tag="PKY09_08290"
     CDS             complement(20083..21369)
                     /locus_tag="PKY09_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12894.1"
                     /translation="MEMFFIPLVYILLICLLIGNHRFFELKGVSRAFVCILFIIKAFA
                     GIIITFNAEKIYSGDDGYFFYEAGKTFLDYGVENPFEYLKLMFGIYDSSNSEAYHHFL
                     NWKNLPMWNDGETMIKLCSLIHIFAFSNILVHSIVFSFLSFCGLTAIYKATIHITRSN
                     GFSLFLLLMLWPSVVFFSSGILKETLLIALSGLFFFSLTQFGVSLRMKIYCFMLFFLL
                     WLIKPHFIFFFTPAIIAYISMTKFKLKSNWVSRLVILGVFALYIVLSVVVHDKILGRN
                     AASTIALVQQSSMKNSIYEKAESYIQPPVIAADYGSILRNTPKAFYQVITVPWFYTNA
                     GLRWKTAAIENILVLIMVLMNIYAAFDRKNKYTLYHFAFLLFLISFYSLVGFTCALEA
                     SILRFKAPILPFLIAGSYMFLLQYKDKILKNATSDN"
     gene            complement(21371..>22005)
                     /locus_tag="PKY09_08295"
     CDS             complement(21371..>22005)
                     /locus_tag="PKY09_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12895.1"
                     /translation="LLFLMMIKFHNFILLLPLFAAYLITSVYRKKTLLIFVLIVSVYY
                     LILINLNLVLPGFGLMELLSNKQAEFVDLANDYHANSAIAINHLTPSEWNAIANTPQA
                     LWHSFFRPYPFESKNLFYQISGFENLFFGLLIILVLINFKKPKSASPLFWVAVFYTFS
                     LFELIGLVTPVMGAIVRYKIQALPFLFFLLIYLTDIEKLRSVFITKSKIT"
BASE COUNT         7571 a         3904 c         3905 g         6625 t
ORIGIN      
        1 ttgccggagt gttttatttt gttttagtca tgttgggcat ggctttgtct attggcagtc
       61 agattttaat gtcgcgcaaa gcaggagaaa acaacagact tgcaattgga aattattttg
      121 accacagcgt tgtgatttta tcagtgcttg cagtcatttt atacttcatc atgttttttg
      181 ccgctcctgc ttttttcaat ttggtgttgc attcaaaaga cgtagccgat gcttcaataa
      241 cctatattca taacagaagt tttggaatat tttttattct tattgtcaat tgctttcggg
      301 cattttatac cagcattagt gcaacacgat ttattactta tagcgcctta gtgctaacag
      361 tgtcaaattt tattcttgac tatgttctca tattcggcaa attgggcttc gaggctcagg
      421 gcattgccgg tgctgcacgt gcatctgcca ttgcagaagt gttggcatgt atctatcttt
      481 tctttatcac attttatcga aatgagaaca gcagctttgg attgtttcgc tttgtcaata
      541 taaatctgac acgcataaat gaaattattt tcatttcaat acctgtgttt ttccagaacc
      601 tgctttctat gggtgcctgg tttttgtttt ttgtatttat agaaaggctt ggccaacatg
      661 agttagccgt ttctaatgtt gttcgtagcg cctacatggt tttgatgaca cccatttggg
      721 gctatgcttc tgctgccaac agtatggtga gtaaccttat cggacaaaac aaacaggatg
      781 aagtaattcc gcttgtaaaa aaaattatag tcctgagttt agcctcttca accctaatct
      841 tactcattaa tctttttgat tttaaactat tgttgcgcat aatttcttct gatatgatgt
      901 tgctgtacga tgcaacacct tcttatatcg tcacctggtg tgccatgttt tttttcagtg
      961 ttagcatggt gctgctgagt gccatcagtg gtacaggcaa aactaatgta gcatttataa
     1021 ttgaagtaat aaacatcgcc atttatgttg cttatgcatt tattacagct gtactgctaa
     1081 aggcacagct tcatgtggtg tggtatgtag aaattcttta ttggataata atgggattat
     1141 gttcatggct atatttacaa accaataaat ggagaaaatg aaacacgttt tgctgatttt
     1201 atttatttac atgctggctt cctgcaaatc tgcaaagcag gaaacaatac atcaaaatct
     1261 tgaaacagta acagtgactg acatgagaaa cttggatggg tgtggttttc ttttaaaaac
     1321 tactgatcag aaaatgctga tacccgataa tcttgatgat tcatttcaaa aaaataattt
     1381 aaaacttgaa atcagctata aaaaaacttc ggtcatgact acctgtatgg cagggcaaac
     1441 cataactctc ttatattgcc gaaaagcaaa atagttatct tagcaataat gaacgacttt
     1501 aaaatacttt tttccggaac gcctgacttt gccgttgagt cactgaaaat tttggtcgaa
     1561 aacggatata atatttgtgg tgtgattaca gcacctgata aacctgcagg tcgtggcatg
     1621 caaatgcaac aatcagccgt aaaaaaatat gccctgtcaa aaggcttata cattcttcaa
     1681 cctgaaaaat taaaagatgc agtatttctt tcagaggttg catctttaaa agccgattta
     1741 catatagtag ttgctttcag aatgatgccg gaacaactct ggagtatgcc acgcttaggt
     1801 acattaaacc ttcatgcatc tttactgcca caatacagag gtgctgcacc aataaaccgc
     1861 gccatcatga atggtgaaac cacaacaggt gtaacaactt tttttctgaa gcatgaaatt
     1921 gatacaggag acataattct ttccgataaa gttgatatcg gacctacaga aaccgcaggc
     1981 gaactgcatg acagactcat gcatattggt gcagccttag ttctgaaaac tgttcagcaa
     2041 atagaaaaag gaaatctttc cactacgcca caaaaaagca taacatcaga gctaaaacat
     2101 gccccaaaaa ttttcaaaaa cgattgtata ataaattggt ctgataaaac ttcaaccgta
     2161 tttaatcaga tacgcggact gtcgccttat cccggagcat ttactatgct tgaagataaa
     2221 aatgggaaaa cactgacttt aaaaatatat tcagcattat ttttagaaag caaagaatta
     2281 tcagagacag ggaaaataat ttccgacaac aagagttttc tgaatatcac tacgtctgat
     2341 ggcataattt caatcaaaga gttgcagctt gaaggcaaaa aaagaatgtc tgcagaggaa
     2401 tttctgagag gcttttccat taacgattac actgttaaaa aataaatcct tctcttaatt
     2461 tttgtggtga ctgaaacacc aaatttgcaa ggcagaaaat gaaacgaatt gtagtattaa
     2521 caggtgcagg catcagtgca gaaagtggta taaaaacctt tcgtgatagc aatggcttgt
     2581 gggaagaaca tcgcattgaa gatgtggcta caccgcaggc atggcagcgt aaccctaagt
     2641 tggtgacaaa cttttacaat cagaggcgta aacaggtgat ggaagcactg cccaacaaag
     2701 cacatttggc tctggttgag ttggaaaaac attatcaggt tgtagtaata acacaaaatg
     2761 tggacgacct gcatgagcgt gccggctcaa aacatatcat tcatttgcat ggagaaattt
     2821 tgaaaatgcg cagtgaagaa gatccctcaa tgatttacca atccaatgga tgggaaatgg
     2881 attacgaaag cacaaacgac cacggacaca gacttcgacc tcatattgtt tggtttggtg
     2941 agatggtacc caaaatggat gaagccatat atcaaaccat gaaggcagat atttttttag
     3001 tgataggaac ttcgctggaa gtttatccgg cagcaggcct tattgactac acatcagata
     3061 gctgcgaaaa atatattgtt gaccccaaag cccgggagtt gaatcatatc caaaatctta
     3121 cagtaattca aaatacagca ggagagggtg tgcctgcact tgtaaacaaa ttaattaaaa
     3181 atgtttagcg aagcgtttat gcaaaccgac ctttacagtt gggtcgttct tccggtattg
     3241 attttccttt gcagaatgtg tgatgtaaca ttggcaacat tgcgcaacat attcctttca
     3301 agaggggtta gaaaaattat tcccataatt ggatttgtag aggtgcttat ctggctgctg
     3361 gcagtaagtt caattattaa aaacctgcat aacataatgt gctacgtagc ctttgccggt
     3421 ggctactcta tgggaatatt ggtaggaatc agcattgaaa aaagattggc tttaggcaca
     3481 caaattatca gagttattac aagcaggcaa agtgaaaagc tattacaagc cttacgtgcg
     3541 caaaatttgg gagtaactga gatagatgct atgggttcgc aagggcctgt aaagatttta
     3601 ctcattgtag caaaaagaaa aaatattgct tccatattag agatagttaa tgaacatcat
     3661 ggcagctcgt tttttacaat tgaagatgtc cgctctgttg aacaaggcat atttccaata
     3721 aaggccggtg aaggaaaaat tcaatacctg cgcaggattt tcccgacctt taaaccggga
     3781 cgttgaaaaa ataatttttt atttaatgtt tgattgttgt acttttgaaa ggagttaaag
     3841 aggtgctttt atgtcaacaa ataaaaccga ttccaaagaa agatcaatca gagtttctag
     3901 gattgaaaga atacatcctt acagaacaat tctgtttttc ggcatactga taagttcagt
     3961 gttatttcta acattatcat ttttgttttt aataacacaa aacgaaaaca gcaatctgcc
     4021 gcctgcattg cctaaagttt ttacactaag cacaatcttg ttattgtttg gaagctacac
     4081 catctccaaa gcagttagtg ctttcagaaa agattcattc gataaattat taagggcaac
     4141 cattttaaca acaatcatca cttcgctgtt tcttgtgact cagattatcg gattacatgc
     4201 catgcaaaaa acagggtatt ataccaatat gccatttaac tcacattatt ttttcggcat
     4261 cattgcatta cacatggttc atgcattagc cactgttgcc tatcttattt ttatcaccta
     4321 caatgcattt catcagtcgc atgatgtaat tgactcttta ttatttttca gcgatcaggg
     4381 gcaattacta aagttaaaca gtgcaatggt gttcagccat tttgtaaact tcagttggct
     4441 tgctattttc cttatgtttc tcttttcttt ttaatgatta gtaaaagtca tgcagtaaca
     4501 ttagcgctgc tgcttacttt atttttttcc tgcaaaaaag aaaatttaga tttagtcttt
     4561 gaggaaataa atacacctgc aaattttaat cttaacaatc tttttatttt aaacgacagc
     4621 gttgtgtttg cctgtggcgg acaaacggga aatggcataa tactccgttc ctcagatggt
     4681 ggcaaatcgt ggcatatatt gaatgaccaa tttgaatata acatacacag tatatatttt
     4741 ttagacaatc aacttggata ttgcggagga gactctactc atgtttatca aacaaaagat
     4801 ggtggcatca gctggcaatt gcatttcgac tgggaaggaa ttccgtttca atacagctct
     4861 ccgctgcgca atgtttgttt tttaaacaaa gacacaggat tttttgcagg aggaaaaaat
     4921 ttcgaccgtg gcattattta ttcaaccgct gatggtggtg aacactggaa tgtctccggc
     4981 tttgaacatg aacttcgatg catcactcct actgcagcag gcattctatc gggtggatat
     5041 ggcatcatgc ttttaagcaa acaaaacttt gaattcgcat cgctggattg tgacagatct
     5101 ttttatacag gcattgcttt cagcaattta ttagaaggcg ttgcctgcag ctacaatggt
     5161 gctttatacc atacaagtga tggtggaaat acatttagta taaaagacaa aaagaatcat
     5221 acttttggaa aaaacgagca atttttatgc attgatgccc gaaaagaaaa aattattgca
     5281 agtggattaa gtggatttac agccgtaagt actgatgggg gtgaaaactt tgattcgggc
     5341 tatagtctga ataattcaag aataaatagt attaaatttt tgaacgacac aattgctatt
     5401 gccatttctg ataacggtaa agttttcaga attcacctct gatattactg aaatttgaaa
     5461 taataggatt aaacaccctt tcaaagcaaa acatcatttt tctgatgcta tcctttttta
     5521 atacatgact ttcttggtta ttttatttca agacattgca aacagacatt gtgccacagg
     5581 ttgcactgat tgataaattg catgttgccc ttattattaa ttcggctatg ttttttaggt
     5641 gataccttaa caatgaaaac tgctatccgc gtaagtctta atatgtacgg caatttagtt
     5701 atggcatttt actaagtttc tatttttcac attaacggat taatgcgatt tctttgacat
     5761 catacaaatg cttttctcca ttttctcttt caacaatcag taaacccatt gatgacagcc
     5821 cttttatttt accctgaaat tgtccgtctg catctttaaa caatgctgtt tcattcagct
     5881 tatatagatg agagagatat aactgatcaa tttcagcaaa ctttttttgt tgcaacatct
     5941 gataattcac ttcaatgttt tcatgcaatt ttacagcgtt gatttgtgtg tctgctttaa
     6001 atccggtgtc cataaacaca gaagtggcag cgtaaagttc cttttcaaac aactgctgat
     6061 tgaagttgat tccaattcca actatagaag cataaatctc tgaaccacgt aaaatgttct
     6121 caataagaat tccggccact tttttaccat caatcagaat atcatttggc cattttattt
     6181 gcaccttatt agaataaaca gacaacaaat cgcatgctgc caaagccaca catttactca
     6241 gcataaaagc ctcagttact tttaaaaact caggataaac aacaatactc atcagtagat
     6301 ttttgcccgc ctcactttgc caactactac cccgctgccc acgtcctttg gtctgataat
     6361 cagcagtaac tacagagcct tcgggcagct tttctgacat gagcttttca tgtaaaaaac
     6421 tgtttgtgga ctccgtttca tggagattat accatataga atttttaaac atgacagcaa
     6481 acatagttac tttccttatt ttttttactt ttgtctctga ctcaaatcaa taatttttac
     6541 aatttggcaa aaagaacgaa tcctgcaaag gataaagatg aaactacgcc tgtgaaagca
     6601 gccacgttaa aaaaaaccaa ggcaaggcaa cccaagcgta aattaaaaga taatacagat
     6661 aaactactca ctgtaattat tgacagtata gaggataaaa aaggagaacg tattgttgtc
     6721 ttaaacctga aagaactaag taatgccatg gcagattatt ttatcatttg tcaggcatca
     6781 aataaaacac aggttgaggc aatagttcgc aatatagaag aaaaagtaga gaaaaagtta
     6841 aaagaaacgg cagcacacat tgaaggcaaa caaaatgcag aatggatact aatggattat
     6901 tttaatgtga ttgtacatgt atttttggca gataaaagag acttttattc gctcgaaaaa
     6961 ctatgggcag atgcaccatt ggtaaaattt gcagcccaat aataaacaaa actgccattt
     7021 tgccgtttgc atgattgttg attacaagaa aaaccaaaga ttgaataaaa aagatgtcag
     7081 aagagaataa aagcggagac aagaaaaaat caccatttgg caaaatgcca aaacctcccg
     7141 ggaagggttt taatttttcg tggatttatg ccatagtatt cctgttgatt tttgtcattt
     7201 gggtattagg ccttggcgat aaacccaaac tgaccaacat gaatgagttt gaaaaaacca
     7261 tgctagccac tcatgatgta gaaaaaattg aaatcgttaa tcgtgaacgt gtagagataa
     7321 ccattaaaaa agacaagcta aaccaggaga aatacaaagc tgttagccac aaaccctttg
     7381 gcaatggcta caatcccggc ccacattatt atatgcccat tggcgatgtt ggaaactttg
     7441 aatcaaaatt agaagctatt cagaaggact tccctgagaa tgaaagaata caacctgaat
     7501 acaacaacaa cgaccgctca atccttgaag tgttgttttg gaatttattg ccgtttatca
     7561 ttattgccgt aatctggatt tttattatgc gtagaatggg tggcggtggt gccggcagcc
     7621 aaatattcaa tatcggaaaa tccaaggcgc aactgtttga taaggacttg aatgttaagg
     7681 tcacgtttaa tgatgttgcc gggcttgaag aagccaaagt ggaagtaatg gagattgttg
     7741 actttcttaa aaacccaaaa aaatacacgc aacttggggg taaaatacca agaggtgccc
     7801 tgttggttgg ccctcctgga acaggtaaaa cacttttggc aaaagcagtt gccggtgaag
     7861 cacaggtacc atttttctcg ctttcaggat cagactttgt agagatgttt gtgggtgttg
     7921 gtgcatcacg tgtacgcgat ttattccgtc aggcgaaaga aaaggcacca agcataattt
     7981 ttattgatga aattgatgcc ataggacgtg cccgaggcaa agcgccatct ttcagtgcca
     8041 atgacgaacg agaaagtaca ctcaaccagt tattgacaga gatggatggt tttggcagca
     8101 acacaggtgt catcatccta gcagcaacca accgtgccga catcttagat cgtgcattgt
     8161 tgcgcccggg acgttttgac agacagattt atgttgagct tcctgacctt acaggacgcg
     8221 aagaaatttt caaagtacat accaaagcat tgaaaattga tcatactgtt gatattaaat
     8281 ttcttgcccg ccagactccc ggattctccg gtgctgatat agccaacctt tgtaatgagg
     8341 ccgctcttat agctgcacga aaaaataaaa cagcaatagg aaaagatgac tttctttctg
     8401 cagttgacag aattattggt ggtcttgaaa agaaaaacaa aatcatcacc aaacatgaaa
     8461 agagtgttat tgcttatcat gaatcgggac atgcaaccgt aagctggcta ttggaacatg
     8521 ctcatccatt ggtgaaagtt actattgtgc caagaggaaa ttcattaggt gcagcatggt
     8581 atctacccga agaaagacaa ctaaccaaca aagagcagat ttttgatgag atatgtgctg
     8641 ctttaggtgg tcgtgctgcc gaagaagtaa tatttggaac cataagcacc ggggcgcttt
     8701 ctgatttgga aaaaattacc aaacaggcct atgccagcgt aagcatcttt ggactaaacg
     8761 aaagaatcgg gaacatcagt tactatgatt cttcaggtca gcaagactat tcattcagta
     8821 aaccatacag tgaaaaaact gcagagatta ttgatgagga agtaaaaaaa ctgattgact
     8881 tagcttatca gcgtacaaaa aaactgttgg ttgataacaa agaaaaatta gacaaactgg
     8941 cgcacaggct tcttgaaaaa gaagtgatct tcaaagaaga tttagaggaa attttcggtc
     9001 atcgtccgtg gggcgagtca gaaaaagcca ctttcaattc tttcataact gatggcgagc
     9061 ctgaaccgga aaaaactatt cctcctgcag ttgacagtcc tcctccatca aatattcctc
     9121 aagggtcaat tcaggtataa tttatggacg aaagtaccaa cagagaaaaa gttctaaaaa
     9181 aggtacgcag tgcattaatt gctaaaacac ctaatccgta tcccaatctg gatatggata
     9241 agggtgtcta taattccatg tcctattcac cggaagtaca gtttgccgaa aactttacgc
     9301 agcaaggtgg gcagtttgtg ctttgcaatg gtcgtgttga actcatggaa accatacttg
     9361 gcgtatgcga tgccaatgat tttactgagg ttgtttgtgt tgaccgcaac ttatcagcct
     9421 tttttgatga gtttgaattt ccacatcaaa gtgtgttttc cgaagatgat gacacagaca
     9481 ttcaggctgt cattattccc tgtgaatgct tagttgtacg gaatgggcta attgttttat
     9541 ctgaagataa catttaccaa aaggctttgc ttagggtaac acagcactta gtcatcatag
     9601 cacatactca gcaacttatc cctgatatga ccgaagcatt ggcatatgta aggtatcaaa
     9661 atgattctaa tgtgccttct gaactttact ttgcaacccc aaactttttg aatcagatac
     9721 agaatgcagt agcggctaat gcctcagtta aaagcatgac tgttattctg attcaacctt
     9781 acgatagtga attataatat ttctattata aaactaaatt attcaggttt atgaaaccaa
     9841 ttaattattc atcaacaatc tacatttcaa gataaattca aaataaatga catatatcaa
     9901 tagtaacatc aactatatta acgtaatttg taatgtacat atcatagata tcagtcaacc
     9961 ccaacattat atcataaata tttaatatcc atgagtcatt ctgatttaca tcgttttcat
    10021 attcccgtta tgggtcttgc ctttacggtt gatacgcctg ttaaggtagc tcgttatggg
    10081 atttcatctg ttgtttctat tattgaacac aacatgcttg aagttatgcg tgagttttat
    10141 tacaaacaaa ccggaaaaga atttacagca attaccaaaa aagaagaaga ttatcgtgct
    10201 aaaagagtaa cagcatatct taacattatc aatgaaattg ttttaaaaca aattactgat
    10261 ttacgtaatg agtcgtttga aacaggtaaa gaaatcaata aatattttga aatgttacct
    10321 gacacatctg aagcaaagct tctttacaga aaaatgcagg ctagtgcaaa tcaggtagag
    10381 aaagaacaga tgcaacaaca tctacgcacg ctcataaaga gtggcgctat tgatgtaaac
    10441 atcatgacaa aggttgacag aatcaactac gataaaaatg aacaaccatt agcacaagaa
    10501 ttttctgatg cgcacaattc attacggggt tttgcacata gtaacctgaa ttcctctatc
    10561 gttttttctg caggacttaa tccacgctta tactcctact gcgaaaagtt tgatgatttt
    10621 ttccctgatg aatcaggatt tgttaaaaag aaaattacac taaaagtcag tgacttcagg
    10681 tctgcacaag tgcaaggacg attctttgca aaaaaaggaa tatggatttc tgaattcaga
    10741 gtagaatcag ggcttaattg cggaggtcat gcttttgcta ccgatgcttt actgttaggt
    10801 cctattcttg aagaatttaa gcttaacaaa aatgcattgt atgatgagtt gttcaacact
    10861 tgtcaagctg ctttggcaca gaaaaacaaa ccacagtata aagagaaacc taagatgaaa
    10921 gttactgttc agggtggcat tggtacatct caggaaaacg attttcttta tcatcattat
    10981 gaggttgacc ttaccggctg gggaagtccg tttttattag ttccggaggc tactaatgtt
    11041 gacaaggaaa cattaggctt attggcaact gcaagtaagg acgactatta tctgagcgaa
    11101 gcatcaccgc ttggtgtgcc gtttaataat ttcagaaaat catctgctga aagacagcgt
    11161 aaagaacgca ttgccaaaaa cagacctggt agtgcatgct ataaaaaatt tcttgcattc
    11221 aatactgagt atggtggaga acctatctgt acagcatcaa gaaaatatca gcatcttaaa
    11281 ctacaggaac taaaacaagc aaatgtggag ccgtcacaaa aacaaattga cgaccttctt
    11341 attaaagatt gtctgtgcga aggattggct tcccctgtta tgctggtaaa caatgtactc
    11401 gttccacata atctgaacgc tgtaactatt tgtccgggac caaatctggc atatttttca
    11461 ggtacattta ccttgcagga aatgataaat catatttatg gtcgagcgaa tgttctgaac
    11521 acattatatc gcccacacat gtttatcaat gagctcaacc tttatattga gtatctcaaa
    11581 aaagatattc aaaaaagtgc tgctaaaata aatgataaga aagaaaaaca attgcaacta
    11641 ttcaaagaaa acctttcaaa aggaattgaa tattatcgaa atctaagtaa caaactgtca
    11701 aattttacaa aaacaaacat tgaaaatttt cagaaagatt tacaggagtg tgaaaatatt
    11761 cttaagtcaa tgttggtaga agaagttcaa tattaatttt ccattcttta ttgttttatt
    11821 taaaaaacac aatattcttt ttaaagtata taagcctgat gtttttaaaa tagttgtctg
    11881 aacttataat gtcaataaga actaagaata caactatcta aacttctttt actcttattt
    11941 ttatcctaaa ttcggctttt aaaataaaag aatctttgga caattatgac ttgcggaaaa
    12001 tttgttgtat cacttgactt tgaactcctt tggggcgtaa gagataaaag aaccattcaa
    12061 tcttatggag aaaacttaag aggggtgcat caggcaattc caaggttatt aagtcttttt
    12121 gataaatacc aaataaaagg cacctttgca acagtaggat ttttattctt cgaaactaaa
    12181 gaagagttat tagcaaatat tccgaaaaca attccaactt atacaaacac tgaattatcg
    12241 ccttatcaag ggcattttaa tttgttaggt gaaaattaca tagaagatgt ttatcatttt
    12301 gcgccacaat taatcaatga aataaaaaag tatccggaac aggaaatcag cactcataca
    12361 ttctcacatt attattgttt ggagaacgga caaacaaaag aaaactttaa agcagatatt
    12421 gaagcggcat tacatattgc tgaaaaaaat ggcattgtaa taacctcact cgtttttcca
    12481 agaaatcaat ttaatgaggt atatataaat attattaaag agcttggact taacagctac
    12541 agaggaaatg aaaactcatg gctctataaa gcaaaaaagg gcgaacaaga aagttctttt
    12601 cgcagagcat taagattaat tgatgcctat tttaatatct ccgggcataa ttgttatagt
    12661 gatgactttt taagaagtaa atatcctatg gatattccat caagcagatt tttgagacca
    12721 tatagtaaat tacttagtgc tcttgaagga tttagattaa aacgtattct atcaggaatg
    12781 acatttgcag ctaaacataa tcagacctat catctttggt ggcatcctca taattttgga
    12841 gtaaatcaaa acgagaattt ttcattccta gaaaaaatac ttgaacacta taaacatctg
    12901 aatattaaat atggtttcaa aagtattacc atgtcacaac tttcaacgga attaaataaa
    12961 aagaggcatg agtgaattag tcaagttcag caaaataaca ctacaggatt ttccaatcat
    13021 tcaaaaaata gctcttgaaa acggaggcag caaaacaatt aatgagtcaa attttaatca
    13081 ttggtattta aaaaatcctt cggcatcaaa ttcaatattt aaggttgaag tcgaaaacag
    13141 tattgaggga tatgcaacaa caaataattt tgtttataca gttgaaaaca aaaattatct
    13201 cgttgctctt ccgcaaaatg ttctgacaac tgaaaaatgc agaggcaaag gattatttgc
    13261 caagttatat tatttaacag aaaaagaaaa tttagagaca aacaaagttg attttttcct
    13321 gacatcaaca aataccatga gtacccctat ttttctgagt aagtttggct atttaagagg
    13381 tttctgccca ccaattctgc taaagttatt ttcgccttta agtctttttt cgaaagcaaa
    13441 ttataaacaa caggaagata taaatgccat aaagattgat cataacgatt taaataattc
    13501 aagaaaaaag aatctggcat attttaaatg gcgctactcc caaacgaata aaaaaaatct
    13561 gacagtcatt tcaattattg aaaataatca gattattggt tatgcttttt taattaaaca
    13621 aattaaaagg ggatttaaag ttttaatgtt agctgacatt atctgcagca acgaaaaatt
    13681 ttattcaaaa attattaaag catgccacac ttacgcttca aagaatttct atgtcggcct
    13741 tctcatgttt gaacttcaaa ataacaatat caatcaagga agaagtatta agttaaagaa
    13801 taaatttaat tttttggtaa agggcaaaag tgatgatgaa actaaaaagc tcagtcaggt
    13861 taaattcaat tatttttttg gtgacttaga ctacttttgg taatctatat cgaaaaacaa
    13921 ttattaatac agcattataa aacaaaaagc agtttaacat aaaatatgta aaactgcctt
    13981 tgtaaaaaaa tcaccttaat ttaaaaggct ctaatttttt cgaatcatca ccttttgtgt
    14041 ctgactaaag ttatcagtag taattgtcag aaaatacaca cctgcactaa gcaattgtgt
    14101 gcctatcatt ttctgatggc ttcccgtact ttgaacacct tgcgtttcat tcatgacttc
    14161 aactcctttc atgtcggtga gtttaatcaa aacttgtcct ggtttcatca gtgaataatt
    14221 aatcacaaaa tatttctcag ccgggtttgg tgcaacagaa aaattactta acaattctgt
    14281 tgatgcaatg cctacgcttc ttaatgaatc aatatatact actgatgcaa ccgtttctgt
    14341 tcccaaaaca tcagtagtat taatctgcaa tagcggaatt ttctcattgt tggtaaacca
    14401 tttatattct acagctttag gtctgtccat agaataacca aagcctaatg tatcggcata
    14461 tagtgtatcg tgtgcataca attctgtttt aatacgaagt gcattaaagc taccgtatgg
    14521 tgtgttaatt gtgccccaac catcaacatg atttacacgc ttttgctcaa agccataata
    14581 tcctaatcca cttaatgaaa aactccatgc tgcctgacag gagtcaacat cgttaaaagc
    14641 aatcggaaaa ttatataaaa catctttact gctaaatgca ataggtgtct gaaatccgct
    14701 aatagtaatg cctaaacctt gctgtttaaa gtcaccacta cttttgtaaa aaaaactgta
    14761 aggatcactt aatgtaattg gtaatccggg aatagcaggt aaattaccaa aaggaattgc
    14821 aatgtttgac ctgttgctgt taaaaccaac gttagcaaaa taaacagaat aggtgccgcc
    14881 tgttgaacta acactaagaa acgctaaagt gtcctgactt tgtgcaatca aagtactaaa
    14941 gtcccaggtt tgatttgctc ctgttgtagt aaagtcaatt ggcatggttg catcagcatt
    15001 gctgactaaa atattatcac ctgcacctgc aaagtcactt tgggtgaggg taatttgtgc
    15061 atgggcgcaa aataccgatg ctaaagcaat catggttgtg taaagttgtt tcatctatat
    15121 gtttatttta aattattatc cgttttctac ccatgactta taataatcag ggatcataat
    15181 actcactgcc tctttggtaa gccaaagtgg tttaaaagta tctatcatca ccgccaactc
    15241 ttttgtctct ttagcaccaa gtgatttttc aactgttccc ggatgtggcc cgtgaggaat
    15301 tccggcagga tgtaatgtaa tttgtccttt tgtaacattc ttacgactca taaaatcacc
    15361 atcaacatag tacagcattt catcagaatc aatattggaa tggttataag gaacaggaat
    15421 tgaaagtgga tgataatcaa acaaacgagg cacaaaagag caaacaacaa aattgtgtgc
    15481 ttcaaacgtt tgatgaattg gtggtggcaa gtgtatcctt cctgttattg gctcaaagtc
    15541 gtgaatcgaa aatatataag gatagcagta tccgtcccat ccaatagcat caaaaggatg
    15601 cgtagcataa acaaaaggat atacaatact ttccttttta atcattacca gaaactctcc
    15661 tttttcatca aaagttttca ggttttgtgg tctgcgaaag tcacgctcac aaaaaggact
    15721 atgctcttca aattgtccat actcatttct atatctgcgc ggtgtttgaa ttgcactaaa
    15781 tgagtcaact accaacaatc tgttattctc agtattaaaa tgtaattgaa aaattgttcc
    15841 tcgcggaatg acaatataat cgccataacc aaaatccatg cttccataaa tagaatggaa
    15901 tgtcccactg ccttcatgca caaaaatcaa ttcatcggca tcggcatttt ttaaaaaata
    15961 atctttagtt gacttacgtg gagcagctaa tgcaatttgc aaatcgttat ttaccaaaac
    16021 aggcttctta cttttaatat aatcatcttc aggttgcaca ttaaagccct gaaaactttg
    16081 atgtaacaac gagcgcttca ttgcaatttc gggttcacgc gaaaatggct ctccagcact
    16141 tttaatcatt gttggcggat ggcaatgata aataagtgaa tacatattgc tgaagccgtg
    16201 agtagaaaac aattcttctg cataaagtcc accatcaggc ttgcggaact gtgtgtgtct
    16261 tttgtgaggg atttttccct gcgaatagta ttggggcatg gtgtgaaaat agtgtcatgc
    16321 aaagttatag taaatcacaa atgaaaccct gaatcatcca acaatatgtt ttggcctgat
    16381 tttcaacagt tgcatgttaa cactaaccgc taatacaacc aataaacagc ccttttttag
    16441 cttaaccttg taaaataaaa tacaatttac ccggttattg aatatgaaca tgttaggcaa
    16501 aagcattgca gttgtattaa tcagcactct tgcattttcg caagacgtga tggcacaacg
    16561 catgaagact gaggattata tcagtcgcta caaagaagat gctataaagg atatgattaa
    16621 aacaggtgtt cctgcctgca taacattggc acaaggtatc ctggaaagcg aaagtggcaa
    16681 cagcaaactt gccattgaag caaacaatca ttttggaata aaatgccata aagaatggca
    16741 aggcaaacaa ttccatcagg atgacgatgc ggaaaatgaa tgtttcagaa aatacaacaa
    16801 tgccttagag tcgtttgatg accattcgga ctttcttcgt tcaagaccac gttatgcatc
    16861 attgtttgaa cttaaaccta ctgattacaa aggatgggca catggattga aaaaagcagg
    16921 atatgccacc aatccgaatt atgcacataa actcattgat ctgatagaac gattcaacct
    16981 tgaagacatc aacaatgaag ccattgcacg aatgaatggc aaaccaattc cggaacagcc
    17041 tacattggca cgcaacgtaa atctacctaa aggatcgcca tctgcaaaag aggttgagaa
    17101 aactaccgtt attaatatta caccaccttc gtattactca gaaattaata atgtaaaata
    17161 tattgtttca aaaaaaggtg atacctggct gagtttagcc aaacaaaatg aaatgatgtt
    17221 gtggcagatg cttaaataca atgatgcatc aaaagatgat gtgctgaaag aaggaactat
    17281 tgtttatata aaaccaaaaa gaaattcagc gaagcaagat tatcatatag tgaaacaagg
    17341 tgagaccatg cgtgatatat cacaattgta tgctattaaa ttaaataaac tttataagaa
    17401 aaatcaattg gaaccgggag cagcacttga accgggcatg aggataaaac tcaataacta
    17461 aaaacaacaa ccaccagact gccgggctaa acgaatgttt ggtccggcat gttttttaaa
    17521 tatgaaagtt gtaatttttt taaactgcat ttttacttta atcataagca atgcttattc
    17581 acagcagact gtaatacctt taagcccact tacaattcct ttagttcaga aaggaaacta
    17641 tggtgaagta agaagtaatc attttcattc cggcttagat ttttctactc agacaaaaat
    17701 cggctttccg gttatagcca gctttgatgg ttatgtatca cggataaaaa cttctgagaa
    17761 tgggtatgga aaagcagtat atatagatca ttccaatggg ctttctacag tatatgcgca
    17821 tctgcatcgt tacggaaaaa ctattgaacc atttgttttt actgaacagg agaaacaaaa
    17881 aaattacagt gttgagttat ttcctgaaag gaataaaatt atgatcagac aaggtgatac
    17941 aatcggttgg tcaggaaact caggtggctc ttccggtcca catcttcact atgaaatacg
    18001 taatacaaaa agtgaaaaac ctttaaaccc catcagctac tttccacaga ttaaagacac
    18061 cattcctccg ctgattaaat caattaccat ctattctatt aaagaaaact ttttaagcat
    18121 ttttccctgg catgaaatca aaccaatgga aacatctcga gggtattaca gctatactga
    18181 attagtaaac atatccggtg aaataggaat aggttttact ggctatgatc aggctaacaa
    18241 tgacaatgca attttaggca ttgacgaagt agaactttat gctaatgaga aattggtttt
    18301 tgcacgaaaa atgagtgact tttcttttga ggaaacacga aatgcaaacg gactaattga
    18361 ctattcaaaa ggtcagcgaa ccgggaacat taccgaaaga tgctataaac ttccgggagt
    18421 aaaattcagt cagtatgttt ttcttaagaa tagcggagtg gttgatgtag aaaatgacag
    18481 caccataaat ttcagactta tgttgcgtga cggttcagga aatatttcgc gttgtgtttt
    18541 tacagtagtg aataacagta atgcaaaacc taaaaacaca gttgtaaaaa aaggagaatt
    18601 gctggaatat aataaggcaa acaaaattca atcagaccga tacaaactat tattaccgga
    18661 gggcgcacta tttcgtgatt tttattgtga tgccataatc cgaagaggaa tcaaatcttt
    18721 tattgctcca caaataacta ttggcagtac atcagaagga ctgaaaagaa atgtaacact
    18781 aaaaattaaa tctgatttag aaggtgatga actcaataat aaaaccataa tggtaaagat
    18841 tgcttctaac ggaacaacat caccggtatc aggaatttat aaagatggtt ggtattcatc
    18901 aagcatcagt agttttggca cttactcact catgattgac agcattgctc ctgtaattga
    18961 aaatattact acttcatacg actcagttta taattgtccg gtacttgaag ttaaagtatc
    19021 ggacaatttt tcaggaatca agaattattc ctgcacaatt aacaataaat ggtacttagc
    19081 ggaatatgac agaaaaaaca gtacaatcta tgttttatta aaccgattca cagagaatga
    19141 attcaatttt aagattgatt taactgatgt atgcggtaat acaacaatct tttgtaagga
    19201 aattatttta atcccgaaat aagatcttaa atttcgctta gtttataggt ggagcttaca
    19261 gccacacctt tttctgtttc cataagatta caacattcgc taaccgggtc gtgttctaaa
    19321 aataaattca aattgttatt cactgcatat ttcaaaaatg ctttcttctc ttctaacgta
    19381 acgagtggtc ttgtatcgta tgccataaca taaggtaatg gaacatgtgc agcagaaggt
    19441 aacaaatcag ccataaatat caattttcca tttttatatg gaatcaaagg caaggtcata
    19501 ccatcagtat gacctctggc aacaacgagg tcaatgttgg aatggaaatt gttcttacta
    19561 attatttcaa atccgttcga catatctgat gttccttcaa aaaactttaa ttgtccactc
    19621 tcttttatag gtaaaatatt ttcttttaaa aagctagctt tctctcttgc atttggctca
    19681 gtagcccact tccaatgatc agcatgacac caatatgttg catttttaaa tgcaggtaaa
    19741 tatccatctc ttgcagaatt ccatttaata cttcctccgc aatggtcgaa gtggagatgt
    19801 gtcagaaaaa tatcagtgat gtcatcggaa tgataacctg cctttgacaa ggacttctcc
    19861 aatgtgtcgt caccgtgaag gtaatagtgt gaaaaaaact tttgactctg tttatctccc
    19921 atactgttat caatcaaaat caacctgttt ccatcttcaa ccaacaggca acgcaaagcc
    19981 catgtacaca tattattatc atctgccgga tttaatttct gccagataga tttaggtacc
    20041 acgccaaaca tagcaccacc atctaattta aaaaaacctg tatcaattgt ctgaagttgc
    20101 attttttaaa attttatctt tatactgaag taaaaacata tatgaacctg caataagaaa
    20161 tggtagaatt ggtgctttaa accgcaaaat ggatgcttca agcgcgcaag taaaaccaac
    20221 aagactataa aaagaaataa gaaacaacag aaaagcaaag tgataaagtg tgtacttatt
    20281 ctttctatca aatgctgcat atatattcat aagtaccata ataagcacca ggatattttc
    20341 aattgcagca gttttccatc gtaaaccggc attagtataa aaccagggca ctgtgataac
    20401 ctgataaaag gctttgggcg tatttctaag aatactacca taatcagcag caataaccgg
    20461 aggttgaata tagctttctg ctttctcgta aatagaattc ttcattgaac tttgttgcac
    20521 taaggctatc gtactggcag catttcgtcc taaaatttta tcatgaacaa ccacactaag
    20581 aacaatgtat aaagcaaaaa caccaagtat cacaagtcgt gaaacccaat tagatttcaa
    20641 tttgaactta gtcatactta tatatgcaat tattgcagga gtaaagaaga aaataaaatg
    20701 cggctttatc agccaaagca aaaagaatag cataaaacaa taaattttca tcctcaagga
    20761 tactccaaac tgtgtaagac tgaaaaagaa caaacctgat aatgcaatca aaagtgtttc
    20821 ttttaaaatt cctgaactaa aaaatactac agatggccaa agcataagca acagaaataa
    20881 actaaaacca tttgaacgag taatatgaat ggttgcttta taaattgctg tcagcccaca
    20941 aaaagataaa aaactaaaaa caatagaatg aaccagaata tttgaaaaag caaaaatgtg
    21001 tatgagtgaa cataacttaa tcatagtttc tccatcgttc cacatgggca ggtttttcca
    21061 attcagaaaa tggtgatagg cctcgctatt agaactatca tagattccaa acatcagctt
    21121 cagatactca aaagggtttt ctaccccata gtcaagaaat gttttgcctg cttcataaaa
    21181 aaaatatcca tcatctccac tgtaaatttt ttctgcatta aaagtgatga taattcctgc
    21241 aaatgcttta ataataaaaa ggatacaaac aaaagcacgt gacactcctt taagctcaaa
    21301 gaatctatga ttaccaatta aaagacaaat tagtaagatg taaactaaag ggataaagaa
    21361 catttccatc ttatgtaatc tttgatttag ttatgaatac acttcgcaat ttttctatat
    21421 ctgtcaaata tattaataga aagaatagaa aaggtaaggc ttgaatttta tatctgacaa
    21481 ttgcccccat aacaggtgtt actaagccaa tcagttcaaa caaagaaaat gtataaaaca
    21541 ctgcaaccca aaacaatgga gatgcagatt ttggtttttt aaagtttatc agaaccaaaa
    21601 taattaataa tccaaaaaat aaattttcga atccggaaat ctgataaaac aaatttttac
    21661 tctcaaatgg atagggtctg aaaaaagaat gccacaatgc ctgaggtgta tttgctatag
    21721 cattccactc agaaggagta agatggttta tggcaatggc actatttgca tgataatcat
    21781 ttgcaagatc aacaaactct gcctgcttgt ttgacaacaa ttccatcaac ccaaaacccg
    21841 gtaaaaccaa attaagatta atcaatatca gataataaac tgaaacaatc aaaacaaaaa
    21901 tcaataaagt cttttttctg taaacagaag taataagata agctgcaaag agtggcaaca
    21961 gcaaaataaa attatgaaat ttaatcatca tcagaaaaag taaaa
//
LOCUS       DAOQNP010000053        21753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_819_length_21753_cov_7.773159, whole genome shotgun sequence.
ACCESSION   DAOQNP010000053 DAOQNP010000000
VERSION     DAOQNP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21753
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_819_length_21753_cov_7.773159"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..646)
                     /locus_tag="PKY09_08300"
     CDS             complement(131..646)
                     /locus_tag="PKY09_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12896.1"
                     /translation="MIRKIIVTQALLFCSASFLFAQHTLVLKSGEKMTGTVQQIKDGN
                     ISFLFKGNPMTFKVSEASAIFFDAASETKADNSELSADMRGVKYEMPGRTITKPPKIE
                     NLTMEKGIVVVEISIDKYGHVTKANPGAEGTTTTSSYLLTKAKQAAESVMFNNCPKCP
                     LDTKGTITLTF"
     gene            793..1812
                     /locus_tag="PKY09_08305"
     CDS             793..1812
                     /locus_tag="PKY09_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB12897.1"
                     /translation="MKLVTYQSQRKTALGILLNDKVYNLNELSQRKGKTVLPDNMRDF
                     LAGEQPLMETAMELSQWLSGGNADVDGLSVANVNLLAPVPNPTSCRDGYAFRQHVEAA
                     RRNRRVEMIPEFDQYPIFYFTNHNAIQGPGDIFCMPDHFEQLDFELEAAIVIGRKGIN
                     VKAKDADSYIAGYMIMNDMSARKLQMEEMKLNLGPAKGKDFSTVIGPYLVTPDELASF
                     KTNAKNGHTGNNYNLKMTCKVNGIQVSEGNVGSMDWTFAEIIERCAYGVNILPGDVIG
                     SGTVGTGCFLELNGTGLLNNPDYKPQWLKEGDTVEMEITGLGVLSNTIRKMDSDFSIF
                     ALKKM"
     gene            complement(1875..5240)
                     /locus_tag="PKY09_08310"
     CDS             complement(1875..5240)
                     /locus_tag="PKY09_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNB12898.1"
                     /translation="MQESKIYTPKHKIRIVTAASLFDGHDAAINIMRRIIQSSGAEVI
                     HLGHDRSAQEVVECAIQEDANAIAMTSYQGGHMEYFKYMYDLLKERGCGHIKIFGGGG
                     GTILPEEISELQDYGIARIYHPDDGRAMGLQGMINDMLQKCDFATGVNLNGEIKELTK
                     KNSKAIARLISCAENNHSEFEKLYPAITTSISNKAKQSPVLGITGTGGAGKSSIVDEL
                     VRRFLLEFKDKNIAIISIDPSKRKTGGALLGDRIRMNSIRNSRVYMRSLATRQSNLAL
                     SRYVQEALDIVKAAQFDLIILETSGIGQSDTEITEHSDVSLYVMTPEYGAATQLEKID
                     MLDFADLIALNKFDKRGAMDALRDVKKQYKRNHKLFDIADDKVPVFGTIASQFNDPGM
                     NQLYKSVMNTITAKTGADLKSDYHTTEEMSEKVFIIPPHRTRYLSEISENNRHYDRWV
                     EEQSELAQKLYSLYESMQIIKGAAAEELKKQYDILERSFDGNNKKLLEDFPAKTAEYS
                     KDYYEFKVRDKVLKIKTFDESLSHNKIPKVAFPKYKAWGDLLKWILQENVPGEFPFTA
                     GVFRFKREGEDPTRMFAGEGCPERTNKRFHYVSLGMPAKRLSTAFDSVTLYGNDPDHR
                     PDIYGKIGNSGVSVCCLDDAKKLYSGFNLCDPATSVSMTINGPASIIAAYFMNAAIDQ
                     QCEIYIRQNGLEKEVEKKITDEFSKNKSSRPEYNYDIPEGNNSLGLMLLGITGDKVLP
                     KDVYDKIKTDTLKQVRGTVQADILKEDQAQNTCIFSTEFSLRLMGDMQQYFIDKAIRN
                     FYSVSISGYHIAEAGANPISQLAFTLSNGFTYVEYYLSRGMDINSFAPNLSFFFSNGI
                     DPEYSVIGRVARRIWAKAMKLKYKANERSQMLKYHIQTSGRSLHAQEIDFNDIRTTLQ
                     ALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINRELGLAKNENPLQGSFII
                     EELTDLVEEAVLSEFDRITERGGVLGAMETMYQRGKIQEESLYYESLKHTGAYPIIGV
                     NTFLSSKGSPTILPKEVIRATEEEKQQQITTLKNLHARYASTAPEMLHDLQLTAIHNQ
                     NMFEKLMEVSKYCSLGQITNALFEVGGQYRRNM"
     gene            complement(5389..6777)
                     /gene="lpdA"
                     /locus_tag="PKY09_08315"
     CDS             complement(5389..6777)
                     /gene="lpdA"
                     /locus_tag="PKY09_08315"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNB12899.1"
                     /translation="MNFDLIIIGSGPGGYVAAIRASQLGLKTAIVERENLGGICLNWG
                     CIPTKALLKSASVFEYLNHASDYGITVEGGKADFNAVIKRSRGVADGMSKGVQFLMKK
                     NKIEVINGFGKVKSGKSVEVTAADGSKKTYTAAHIILATGARSRELPNLKQDGKNIIG
                     YREAMSLPEQPKSMVVVGSGAIGVEFAYFYNAMGTKVTVVEFMPNIVPVEDEEVSKQL
                     ERSLKKVGIEIMTNSSVESVTVSGGTCSVKIKSAKGESVITCEKVLSAVGITTNIENI
                     GLEECGIITDKGKVVVNDFYQTNIPGIYAIGDIVKGQALAHVASAEGIICVEKIAGHH
                     PQPLDYNNIPGCTYCTPEIASVGMTEKAAKDAGIGIKVGKFPFSASGKAKAGGQSDGF
                     VKLIFDEKYGELLGAHLIGANVTEMIAELVLARKLETTGHEIIKAVHPHPTMSEAIME
                     AAAAAYNEVIHL"
     gene            6904..7374
                     /locus_tag="PKY09_08320"
     CDS             6904..7374
                     /locus_tag="PKY09_08320"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HNB12900.1"
                     /translation="MKIRIFNIALVVLMGMWQLSIAQNNKKMEYNKLTPQEEKVLLFK
                     ATDRPFTGDYYLKKDAGTYVCRRCNAPLYKSADKFEAHCGWPSFDDEIKGSVKRIPDA
                     DGMRTEIVCSNCGGHLGHVFLGEGFTAKNTRHCVNTSSLKFIPAGAPLPEVIKK"
     gene            complement(7380..8810)
                     /locus_tag="PKY09_08325"
     CDS             complement(7380..8810)
                     /locus_tag="PKY09_08325"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNB12901.1"
                     /translation="MKFSARTSLLLLSMAAFSQTWAQPQVNIVEYANGIYGKTSELAA
                     NGDGRIYAATQLGYIYIINPGGVVDTVPFLDINNKVTPTSLSNTGEQGLLGLAFSPNY
                     ATDSCFYVCYTNKTGHGNSVLARYKRSADPNVADTASAEIIFALAQPYTNHNGGCLKF
                     GSDGYLYLALGDGGDVGDPGNRAQNMNMYFGKILRIDVSQPGAYTIPSTNPFFGVMNI
                     KNEIWASGFRNPWKFSFDRLTHDMWIGDVGQDIWEEIDFQPANSTGGENYGWRCYEGL
                     VTYNTTVGCTGNYVSPVHVYSHASTGGCSVTGGFVYRGTSYPALYGYYFFADFCNGTI
                     YALDPNNGNAVTVAGTFPGRAFSTFGEDDNGELFVADLVNQKVYQVTDTTTSITEYGN
                     ELNYATVFFNQNEQSLQLNLNSKNNNQATVQLYDLKGVLIAAWKLSLKSGSNTIKLPI
                     DLTSGIYLSNITCGNASRNIKIPVIK"
     gene            complement(8979..>9176)
                     /locus_tag="PKY09_08330"
     CDS             complement(8979..>9176)
                     /locus_tag="PKY09_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB12902.1"
                     /translation="FNSTSIKSAETTIFNRWGKKVTSYSDWKNGWDAKDNHAGVYYYE
                     IEIQFHNGTSKKYHGALNIIK"
     assembly_gap    9177..9276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9277..11434)
                     /locus_tag="PKY09_08335"
     CDS             complement(<9277..11434)
                     /locus_tag="PKY09_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12903.1"
                     /translation="MKKNFLLFFISILLYSAGYSQSCFTINSILVDACGTPEGENEMV
                     RFTVGASPLQVSSLNVTWPNTSLNFLGICQNATTAQKVADLNATIQSCGYLLEPSNDI
                     LPENSKVILITSENFNTAFNSFANLTDTMYVIFQCIGNTQGHFRNYSATVTTPRILTM
                     NFGAVCSQSVTFYPNLLINQFGQLDTAAQDGSSVGYDSLGNATYYNNGCQAPIISLTA
                     TLQSSATTVCSGDSVILTASNLSGNYSGYFWSGGNGTLSSPNNLVTTYQTNTTFTGTD
                     TIQFSMIGQCNDTITSNIYLIVQNGQPASITGPSTVCTGDSITLTASSGTAYQWSNGA
                     TTQSITVLTPGTYSVTVTGTCGTPTASATIAGGTAPNITVTPGTNVSICAGQSVTLAA
                     SGASTYQWSTGATTSSISVNTAGTYTVSSSNACGTSSVTINVTQTTTPVVNITSNSGT
                     EICPGQNITLTASGASTYVWSGGQTTPSITISTAGTYTVTGTSSCGTASNTITITAGS
                     VPSISISPGTAVNICSGQSATLTASGATTYLWSTGATTASINVSTAGNYTVTSSNACG
                     SDTVVVNVTVTNTPSVSITSTAMVICPGQSATLTASGASSYSWSGGQTGNSITVNTAG
                     VYTVTGTSTCGTASATINLTQGNVPTATISSPSTILCNNSTLTLTATGGDSYLWSNGT
                     TTSATTINTPGIYAVTATNTCGSDTANFTVTSTTIDA"
     gene            complement(11532..11990)
                     /locus_tag="PKY09_08340"
     CDS             complement(11532..11990)
                     /locus_tag="PKY09_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12904.1"
                     /translation="MKKFYFLILSIFMITSSCKEKNKLNDLDWLIGKWEGTARNGNIF
                     YEEWKKQSDTAFVNNNYHFEGDKQVFGGTSRIALRNNRLYYINDSDSLKMLQWEAEVH
                     NPTSMTFHNDKITPYNRITFSLSEQNTWDAMLMGKKDTVKYSLKRKIMPY"
     gene            12133..13566
                     /locus_tag="PKY09_08345"
     CDS             12133..13566
                     /locus_tag="PKY09_08345"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNB12905.1"
                     /translation="MLKRHLSLACFIAFLSSVNLSYGQEMNGIAHSNFAGNMGIEMNP
                     ASIATMPYRFEFNILSGDIFFQNDYIYFPASDIPTGKFARFEPLRHEDYSDHYDKTPK
                     NAYGNLLLKGPSAVWRNEEAAFGIHFSLRSQLSAIKVPYHLAKFLWEGSDYSPQHKIP
                     WNSTSFSAGALVYAEAGFTFAKTILTNRQNITTAGGTVNFLLGMSGTYINSTVLDYNF
                     TDASLLNIKNIEVDYGHSIPNDDKKNYLHPRGAGASMSFGIQHFFNYINQAYKPGMSG
                     IKLKKYDLKLGLSLIDLGFIRFNGNARTFSLKNASAVWPGFDTTKITGYEHADSIFSA
                     KLYGDANISRSGSGFNMILPAAASFQVDWSITPKYYLNLAVIQNIQIFDRSVTRASQV
                     SFTPRFETRRVEVSLPMSLYQYNFFRAGIAIRYKWFVLGSDNLGWWPGLYEMNGLDFY
                     FGFKYTSDIYDNIRFKKGKGGHCPAYK"
     gene            complement(13829..14551)
                     /locus_tag="PKY09_08350"
     CDS             complement(13829..14551)
                     /locus_tag="PKY09_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12906.1"
                     /translation="MKKIIIPILCIATLTFTSCKKKDKDNPHYTCSSCVTQAEALAAN
                     DGSSKGVYKGILIGSSGTIKFSIQNGTNDITATMVVDGVSAILTSTVSWVSGQPYIAP
                     FTGTLNGQAVSITFSVQNNGSTPQIISSNIPGHPNAVFTIIKETSNALVEGFEGTYST
                     TEPETGTFNILLSRPLGQWGGISRENGQTATNDVSGTITSSGDLFDDNNIKVATLSGD
                     DIDGTFTDNSGNTVTVDGKRTL"
     gene            complement(14581..16203)
                     /locus_tag="PKY09_08355"
     CDS             complement(14581..16203)
                     /locus_tag="PKY09_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12907.1"
                     /translation="MKQTLPKFSAVWLASAILFPTALIAQNNETTHELSKPAQKGILA
                     NAELSADGNIRMTYKMKVDKKSDQVNYEDYVFDKNLKFIGVEKTKEDKQTQPDQKVTL
                     IKAFVGGGNSFNVMSMSLNLQQEVWERNWNFDKQKYQWGSRISKEVVKPKNNDSKYRG
                     FAAYGNDDDGSVLILASYDSGDDDQFVCLHVDKGLNMTETKLPLQGKYSLVYCGTLKS
                     GNIFAVFAPNEGMSDTKKYYFIECSADSKVAKSNEFTSPSPNMLIMDYSEANGDLYFI
                     AGSDKSNEAYNKVFTSYAPIDNPGYSTSNNRQMDKYEKRAMETEFTNLHLLRFSDGKL
                     AFASTEPVKDFKTKMVTPPSQKKGQPYTGKKIVIENLFVAPSGDVLVAGQLSDKKVVN
                     GGNSYEFRYYDYVCMQFDNQGKLVAQYAVNKLLDDSKNEVFSAIQNFIPSADGKSVYW
                     EILEVQAAKYYASVAAAYSGNYSLSSHYFPRIANITLSNHSVSNFTELGEKGKYLVYH
                     SITSLTDKDNNRFYIGHDEDYKNIWLGKYTFR"
     gene            complement(16274..16897)
                     /locus_tag="PKY09_08360"
     CDS             complement(16274..16897)
                     /locus_tag="PKY09_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB12908.1"
                     /translation="MTIQVGIIEDEKHIRENLQILIDGSEGFSCKHLFSNAEDAINII
                     PTLQLDVVLTDIQLGGKTGIDCILELKDKCPSTNFLICTSFDDSEHVFKALKAGAMGY
                     LTKTTQPSKLLDAINDVYRGGSPMNSHIARKVVSSFMETPVSPEYEKLSERERLIVDL
                     LSKGMRYKEIADQLFLSTETVRTHIRNIYHKLQVNSRTEALNKVFKK"
     gene            complement(16937..18973)
                     /locus_tag="PKY09_08365"
     CDS             complement(16937..18973)
                     /locus_tag="PKY09_08365"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12909.1"
                     /translation="MSPFLSRIIFCVFLLLSHYVLYASDATPTAQQKLIDARISEAEI
                     IYKSDFKRAYTIAVDAKTKAVNIEYTEGIVASSCLQSIIESQTNKSNIAIEGLNKILH
                     SSKSINLKLRQQLLSTLGACYFYIGKLDSAQYYYNESISSFSSNDTTIQLFKSCLQLS
                     EVFIKENIPSKALDLYGKAYKIYQKNRSKEGEVWLHEVLGKIYYRQNLYEQSISTIKT
                     SIIQYELMNNLTGQITSLIQNGNSYYKQMLDDSAANCYQQALILATTLGDERSKSICY
                     SNLSRIYLEHNQTKQALETALHALEVIQPGNYPEIEAGTYQQLGDIYIELGQNRKAIE
                     WIQKALTLAKDNHNKIIERDCYKSLSELYASIKQYEPAMENLLQAYRIKDSIQPITFY
                     NRLAEMEARYSLEKKESTIKLLNQQSFINQLRLKEQKENIKKQNALVIILLILITTIV
                     IAIYFYLRWRKQAELINQQNTIRQTEENERIRIVKDIHDELGSGLTKIKFLSEILEMK
                     TKDQVQLKETLQTITGTSHQLIDNMRDMIWNMNPMNSTLDNLIARVREFSTEYLEDFP
                     IEVSYAFPENIPSEKINIEISRNILMIVKESLQNIVKHSNASKVSIRIEIQPVFKLII
                     TENGIGFSMNEKTTGNGLRNMSERSKTIGASLNISSKPGEGSILSLEKNLNILL"
     gene            complement(19068..21722)
                     /gene="cphA"
                     /locus_tag="PKY09_08370"
     CDS             complement(19068..21722)
                     /gene="cphA"
                     /locus_tag="PKY09_08370"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNB12910.1"
                     /translation="MRILEIRPMRGPNFWSIRRHKLIVMKLDLEELEERPTDKIEGFL
                     ERLKKMFPSMQSHRCSEDHEGGFFKRVKEGTWMGHVIEHIALELQTLAGMDTGFGRTR
                     GTGEHGVYHVVFSYIEEKAGVYAARASVRIAQALIDNKTYDLNNDIQQLREIREDERL
                     GPSTGSIVEEAASRGIPWIRLNRHSLVQLGYGINQRRIQATIASTTSSIAVEIACDKE
                     DTKNLLEAQSIPVPRGKIIRDEEGLKDAVEDLGYPLVIKPLNGNHGRGATINIVNWDQ
                     AVEALEIAKKISRSVIVEKYIIGYDHRILVINYKFVCAAQRKPAAVVGDGKSTIQQLV
                     DAVNADPRRGYGHENVLTSIKIDEMTQNILVEKGLTLDSILKKGEELFLKRTANLSTG
                     GTATDVTDIVHPYNVFMCERIARIIGLDICGIDIMTPDISEPLTETGGAVLEVNAAPG
                     FRMHIAPTDGLPRNVAEPVVDMLYPPGSNYRIPIIAVTGTNGKTTTTRLIAHIAKTCG
                     HKVGFTTTDGIYIQNQMLQRGDCTGPQSAEFVLKDPTVDFAVLETARGGILRAGLGFH
                     RCDIAVVTNVAADHLGLKGINTLEEMSKVKAVVPESVQLNGYAILNADDDLVAAMARN
                     LDCNVAYFSLDENNAIIKSHCEAGGLAAILENGFVTICKGTWKLRVHKVINIPLTFSG
                     KAVFMIQNILPAILSAFIRNFKIEDIRLALETFIPSPVQTPGRMNMFQFKKFTVMVDY
                     AHNPAGFQAIARFLEKVDAKPKVGVIAGVGDRRDEDIVALGSLAAKMFDEIIIRQDRN
                     LRGRTEKEIIDFMLEGIKSVDANKKVTVIPLEREAIDHAINNGKKGSFIVICSDVIPD
                     ALDQIMKYKEKEDQFEMHLDDIPNQSNR"
BASE COUNT         6195 a         4113 c         4155 g         7190 t
ORIGIN      
        1 gaaacaattt ctgtattaag aagcttggca gcttcaacgg caatttgtgt tttgcccgaa
       61 gctgtaggcc cggcaatgat gattaacttt ttatccttag gcaatttttt attctttgct
      121 tatggcaaag ttaaaaggta agtgtaatag tgcctttggt atctaatgga cactttgggc
      181 agttgttaaa cataacactt tctgctgcct gttttgcttt ggttaacaaa tagctgcttg
      241 tagtggtggt gccttctgca ccgggattag ccttggtaac atgtccatat ttgtcaatag
      301 aaatctcaac tacaacaata cctttctcca tggtaagatt ttcaatcttt ggcggtttgg
      361 tgatggtacg tccgggcatt tcatatttta caccacgcat atcggcactc agctccgagt
      421 tgtctgcttt tgtttctgat gctgcatcaa aaaatattgc agaagcttcg ctcactttaa
      481 aagtcatggg gttgcctttg aataaaaagc tgatgttccc atcttttatc tgctgcactg
      541 tgcctgtcat tttttcgcca cttttcaaaa caagagtatg ttgtgcaaat aagaaagatg
      601 cgctgcaaaa caaaagtgcc tgggttacaa taatttttct gatcatgatt taaacttttt
      661 tcaaaaataa atttttgtag ctgttctaag aatgaatttg aattgaaata tgaatacatt
      721 tttcaacaat agaagcgata ttaggcttct tcgatgcaga atgataattt tggcaccata
      781 aagtattaaa gtatgaaatt agtaacctat caaagtcagc gtaaaacagc attaggaatc
      841 ttgcttaatg ataaagttta taatcttaat gaattgtcac aacgtaaagg aaaaacggtg
      901 ttgcccgaca atatgcgtga ttttttagcc ggagaacagc ccttgatgga aactgccatg
      961 gagttgagtc aatggctttc cggaggcaat gcagatgttg acggactctc tgtggcaaat
     1021 gtaaatttac tagcaccggt gcctaatcca acatcctgtc gcgatggata tgctttcagg
     1081 caacatgttg aagcggcaag gcgcaacaga cgtgtggaga tgattcctga atttgatcag
     1141 tacccgattt tttattttac caatcacaat gctattcaag gtcccggaga tattttttgc
     1201 atgcccgatc attttgaaca actcgacttc gaacttgaag ccgctattgt cattggtcgc
     1261 aaaggtatta atgtgaaagc aaaagatgct gatagctaca ttgccggtta tatgattatg
     1321 aacgacatga gtgcacgtaa gcttcagatg gaagagatga aacttaactt aggtccggca
     1381 aaaggaaagg atttttctac tgttattggt ccttatttag taactcccga tgaacttgca
     1441 tcatttaaga ccaatgcaaa aaacgggcac acaggaaata attacaatct gaaaatgact
     1501 tgtaaggtca atggcattca ggtgtccgaa ggtaatgtag gaagtatgga ttggacattt
     1561 gcagaaatta ttgaacgttg tgcatatggt gtaaacattc ttccaggtga tgttattggt
     1621 agtggtacgg ttggtacagg ttgtttctta gagttgaacg gaacaggttt attgaataat
     1681 cctgactata aaccacaatg gctaaaggaa ggcgatacag ttgagatgga aattacaggc
     1741 cttggagtgc tgtcaaatac catcagaaaa atggattctg atttttcaat atttgcattg
     1801 aagaagatgt agggagcagt gtgaatagtg aagttctctt tgattaaata tatttattaa
     1861 attaaaatcg ttatttacat attcctcctg tactggcccc ctacttcaaa caatgcattg
     1921 gtgatttgtc ctaaagaaca atacttactt acttccatca acttctcaaa catattttga
     1981 ttatggatgg cagtgagttg taagtcgtgt aacatttcag gtgcagttga tgcataacgt
     2041 gcatgcagat tttttaaagt agtaatctgt tgttgctttt cttcctctgt ggcacgaatc
     2101 acttctttag gtaaaatggt aggagatcct ttgctcgata aaaatgtgtt gacaccgata
     2161 atcggataag caccggtgtg ctttagtgat tcgtagtaaa gactttcttc ctgaattttt
     2221 ccacgctgat acattgtttc catcgcaccc agtactccac cacgttctgt tatacggtca
     2281 aactctgaca gcacggcttc ttctaccaga tcggttaatt cttcaatgat aaatgaacct
     2341 tgcaaaggat tttcgttttt ggcaagaccc aattcgcggt tgatgataag ttgtattgcc
     2401 atagccctgc gcacactttc ttctgtaggt gtggtaatag cttcatcata agcattggtg
     2461 tgaagggagt tgcagttgtc gtaaatagca tacaaagcct gcagtgttgt tctgatgtcg
     2521 ttgaagtcaa tttcctgagc atgcaaactt cttcccgaag tttgaatatg atatttgagc
     2581 atctgcgagc gctcattggc tttgtatttt agtttcatcg ctttagccca aatacgcctt
     2641 gccacacgtc cgataacaga atattcaggg tcaatgccat tggagaaaaa gaaagaaagg
     2701 ttgggcgcaa aagagttgat gtccatgccg cgacttaaat agtactccac ataagtaaag
     2761 ccattactca atgtaaaagc aagttgcgaa ataggattgg cacctgcttc ggcaatatgg
     2821 taaccgctta tggaaacaga ataaaaatta cggatagcct tgtcaataaa atattgttgc
     2881 atgtcgccca tcagcctcag agaaaattct gtagagaaaa tgcaagtgtt ttgtgcctga
     2941 tcttccttca gaatatcggc ctgaactgtg ccgcgcactt gtttcagcgt gtcggttttt
     3001 attttatcgt aaacatcttt tggaagtact ttgtcaccgg tgattccaag cagcatgagg
     3061 ccaagcgagt tgtttccttc aggaatatcg tagttgtatt caggtctgct tgatttattt
     3121 tttgaaaatt cgtcagtaat ttttttctca acttcctttt ctaatccgtt ttgtctgata
     3181 taaatttcgc attgctgatc aatagctgca ttcataaaat aggctgcaat gattgaagca
     3241 ggaccattaa tggtcattga tactgaagtg gccgggtcgc aaagattaaa tccactgtaa
     3301 agcttttttg catcatcaag acaacataca ctaacaccac tgtttcctat ttttccataa
     3361 atgtcagggc gatggtcagg gtcattgcca tagagtgtca cactgtcgaa ggcagtgcta
     3421 agccgtttgg caggcatgcc taaagaaaca taatggaaac gtttgtttgt tcgctcggga
     3481 catccttcgc cagcaaacat tcttgttggg tcttcgcctt cgcgtttaaa acgaaataca
     3541 cctgcagtaa acggaaattc acccggaaca ttttcctgca aaatccattt cagcaaatcg
     3601 ccccaggctt tgtatttcgg aaaagctacc ttaggtattt tgttatgcga aagtgattcg
     3661 tcaaaagttt taatttttaa aactttatca cgcactttaa attcgtaata atctttgcta
     3721 tactctgctg ttttagcagg aaaatcttca agaagttttt tgttgttacc atcaaagctt
     3781 ctttcaagta tatcatattg ctttttcagt tcttcagccg ctgcaccttt tattatttgc
     3841 atgctttcgt aaagagaata aagcttctgc gcaagttcac tttgttcttc aacccaccga
     3901 tcgtaatgac ggttgttttc tgaaatttca gaaagatatc gtgttcgatg cggaggaatg
     3961 ataaacactt tttcactcat ctcttccgtg gtgtgataat cgctttttaa gtctgctccg
     4021 gtttttgctg taatggtatt catcaccgac ttgtacagtt ggttcatgcc cggatcgtta
     4081 aactgcgatg caattgttcc gaaaacaggt actttatcat cagcaatatc aaacagcttg
     4141 tgattacgtt tgtattgttt ttttacatca cgcaatgcat ccatggcccc gcgtttgtca
     4201 aatttgttta atgcaatgag gtcggcaaaa tcaagcatgt caattttttc caactgtgtt
     4261 gccgcaccat attctggcgt cattacatac aaactcacat cgctatgttc ggtaatttca
     4321 gtatcacttt gtccaatacc ggaagtttca agaataatca gatcgaactg tgcagctttt
     4381 acaatatcca atgcttcctg aacataacgg ctcaatgcaa gattgctttg acgtgtagcc
     4441 agtgagcgca tatacactct tgaattacga atgctgttca ttctaatgcg gtcaccaagc
     4501 aatgcaccac cggttttacg tttacttggg tcaatagaaa tgatggcaat atttttatct
     4561 ttaaattcca atagaaatct tcttaccaat tcatcaacaa tagatgattt gcctgcacca
     4621 cctgttcctg taattcctaa caccggactt tgtttggctt tgtttgagat agaagtagta
     4681 attgcaggat atagtttttc aaattctgaa tgattatttt ctgcacaaga aataagtctt
     4741 gcaatggctt tggaattttt cttggtcagc tcttttattt cgccatttag atttactcct
     4801 gtggcaaaat cacatttttg aagcatatca ttaatcatgc cttgtaagcc catagcacgt
     4861 ccgtcatcag gatgataaat acgtgcaata ccatagtcct gaagttcgct gatttcttca
     4921 ggtaatatag tgccaccacc gccaccaaat atttttatat gcccacagcc tctttctttc
     4981 aataagtcat acatgtattt aaaatattcc atgtgtccgc cctgatagct agtcatagca
     5041 atagcattgg catcttcctg aatggcacat tccaccactt cttgtgcgct tctgtcgtgt
     5101 ccaaggtgaa taacctctgc accacttgac tgaatgattc tgcgcatgat gttgatggcg
     5161 gcatcatgcc catcaaacaa cgaagcggct gttacaatac ggattttatg tttcggggta
     5221 taaatttttg actcctgcat atcagaatta cctttgtgct gcaaatttaa catttacagc
     5281 gtaagcagga aaggggggag gatacagtta tctaataaga atgataaaca caccttttat
     5341 tgatttaaaa actaaaaaat agttgttggg taacaacaaa tgtcatttct acaaatgtat
     5401 aacctcatta taggctgcag cggcagcttc cataatagcc tcgctcatgg ttggatgcgg
     5461 atggacagcc ttgattattt catgacctgt tgtttctaat ttacgagcca acaccaattc
     5521 tgcaatcatt tctgtaacat tggcaccgat taaatgtgca ccaagcaact caccatactt
     5581 ttcgtcaaaa ataagtttaa caaaaccatc actttgtcct ccggcttttg cttttcctga
     5641 agcactgaat ggaaatttgc caacttttat tccaatgcct gcatcttttg cagctttttc
     5701 tgtcatgcct actgaggcaa tttcaggtgt gcagtaagta catccgggaa tattattgta
     5761 atcgagaggt tgaggatgat gtcctgcaat tttttcaaca cagataattc cttctgcaga
     5821 tgccacatgt gccagtgcct gaccttttac aatatcacct attgcgtaaa tgccaggtat
     5881 gttggtctga tagaaatcgt tcaccacaac cttaccttta tcggtaatga tgccacactc
     5941 ttcaagacct atgttttcaa tattagttgt aatacctact gcagaaagta ctttctcgca
     6001 ggtaatcaca gattcgcctt ttgccgattt aatttttaca ctgcatgttc cgccacttac
     6061 tgtaactgat tcaacagagc tgtttgtcat tatttcaatg ccaacttttt tcaaggagcg
     6121 ttctaattgc ttgctcactt cttcgtcctc tacaggaaca atattcggca taaactctac
     6181 cactgtaact tttgtgccca tagcattata gaagtaggca aactcaacac caatagcacc
     6241 tgagcctaca actaccattg atttgggttg ctcgggcaga ctcatagctt cgcgatagcc
     6301 aattatgttt ttaccatctt gcttcaggtt gggcaactcg cgcgaacgtg ctcctgttgc
     6361 aagtataatg tgcgctgctg tgtatgtttt tttgctgcca tcagctgcgg taacttctac
     6421 actttttccc gactttactt tcccaaaacc attgatgact tctattttat tttttttcat
     6481 caaaaactga acacctttac tcataccgtc agctacaccg cgacttcttt ttatgacagc
     6541 attgaagtca gccttaccac cttcaacagt aattccataa tcggaagcat ggttcaggta
     6601 ttcaaacaca ctggcgcttt tgagtaatgc cttagtagga atacaacccc agttcagaca
     6661 aataccacct aagttttcgc gctctactat cgctgtttta agacctaatt gcgatgctct
     6721 gattgcagct acataccctc cggggccact tcctatgatg attaaatcaa aattcataat
     6781 tgatgcttta atttgctgct aaaatatagc tttttaatga atctgctacc ggtcattaac
     6841 tttgtttctt gtaatgtgtt ttttacatat tgactgagtg gaggtatcaa ataaaaatta
     6901 tcaatgaaaa taagaatatt taatattgcc ttagttgtcc tgatgggaat gtggcagcta
     6961 agtattgcac aaaacaataa gaaaatggaa tataataaat tgacacctca ggaagaaaaa
     7021 gtgcttttgt ttaaagcaac tgacagacct tttaccggag attattatct taaaaaagat
     7081 gcaggaactt atgtatgccg cagatgtaat gcaccgcttt acaaaagtgc cgataaattt
     7141 gaggcacact gtggctggcc aagttttgat gatgaaataa aaggctctgt aaaacgaatt
     7201 cctgatgcag atggtatgcg tacagaaatt gtttgcagta attgcggagg acatttaggt
     7261 catgtttttt taggcgaagg atttacagct aaaaatacca gacactgcgt taataccagc
     7321 tcattgaaat ttattcctgc aggagcacca ctacccgagg taattaaaaa gtagaaagtt
     7381 tattttatta caggtatttt aatgttgcgt gaagcattgc cacaagtaat gttacttaaa
     7441 taaattcctg aggtaaggtc aatgggaagt ttgattgtgt tgctgcctga ttttaaactc
     7501 aatttccacg ctgcaattaa tacacccttt aaatcataaa gttgaacagt agcctgattg
     7561 ttatttttag aatttaaatt cagttgaagc gattgttcat tttgattgaa aaatacggta
     7621 gcataattta actcattacc atattcagtg atagaagttg tggtgtctgt aacctgatac
     7681 accttctgat ttaccaaatc tgcaacaaat aattcaccgt tatcatcttc accaaaggtg
     7741 ctgaatgcac gtccgggaaa tgttcctgca acggtaacgg cattgccgtt gtttggatct
     7801 aaagcataaa ttgttccatt acagaagtcg gcaaagaaat aatatccata aagagcaggg
     7861 taggaagtgc cgcgatatac aaacccgcct gtaacagagc agccaccggt agaggcatgg
     7921 ctatatacat ggacgggcga aacataattt cctgtgcatc ctacagttgt gttgtaggtg
     7981 acaagacctt cataacaacg ccatccgtaa ttttcaccac ctgttgaatt agccggctga
     8041 aaatctattt cttcccatat atcctgaccc acatcgccaa tccacatgtc atgtgtaagt
     8101 ctgtcgaaac tgaatttcca ggggttacgg aatccacttg cccatatctc attttttata
     8161 ttcataacac caaaaaaagg attggttgat ggaatagtat atgcaccggg ttgtgagaca
     8221 tctattctta aaatcttacc gaaatacata ttcatgtttt gtgctctgtt acccggatca
     8281 ccaacatcgc ctccatcacc aagagcaaga tataaataac catcactgcc gaatttcagg
     8341 caaccaccat tgtggttggt gtaaggttga gctaaggcaa atataatttc tgcacttgca
     8401 gtgtctgcaa catttgggtc agcacttctt ttgtatcggg caagcacact gtttccatgt
     8461 cctgtcttgt tggtataaca aacataaaag caactgtcag tagcataatt tggactaaag
     8521 gctaaaccta ataaaccctg ttcacctgta ttgcttaatg atgttggagt aactttattg
     8581 tttatgtcta aaaacggaac ggtgtcaacc acacctccgg gattaattat ataaatataa
     8641 cctaattgtg ttgctgcata aattctgcca tcgccattgg cagcaagttc tgatgtcttg
     8701 ccataaatgc cgtttgcata ttcaacaata tttacctgtg gttgtgccca agtttgacta
     8761 aaagcagcca tacttaaaag caataaagaa gttcttgcgg aaaatttcat taatagtgtt
     8821 ttaaaggaag agcaaatata gttcttgcgc taagacctat ttgtagtaaa atggtaagtt
     8881 taatgaaaat ttgttctttg ctttttcttg aacaaaaaat gccgacttta gtcagcattt
     8941 tttgttttgg agaataattt tttatctaaa acgcaatgtt atttgataat attgagcgct
     9001 ccatgatatt ttttggaagt tccgttatga aattggattt ctatttcata ataatacact
     9061 cctgcatgat tgtcttttgc atcccagcca tttttccagt cagaataaga tgttaccttt
     9121 ttaccccagc ggttaaagat tgttgtttct gcacttttaa tggaggtgct gttaaannnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgc atcaattgtt gttgaggtaa
     9301 cagtgaaatt tgcagtgtcg ctgccacagg tatttgttgc agtaactgcg taaatgcccg
     9361 gtgtgttaat tgttgttgca gatgtagttg taccattgct ccatagataa ctatcaccac
     9421 ctgttgctgt tagtgtcagt gtgctgttat tgcagagtat tgttgacgga gaagagattg
     9481 ttgctgttgg tacattccct tgtgttagat taatagtagc acttgctgtg ccacaggtag
     9541 aagtgccggt aactgtatat acacctgcag tgttgactgt aattgagttt cctgtctgtc
     9601 cgcctgacca tgaataagat gaagcacctg aagccgtcag cgtagcactt tgtcccggac
     9661 agattaccat tgcagtggat gttatagaaa cagatggtgt gttggttaca gtgacattca
     9721 caacaacagt atcagagcca caagcattag atgaggtaac agtatagttt cctgcagtgc
     9781 tgacattaat ggatgccgta gttgcaccgg ttgaccaaag ataggttgta gctcctgatg
     9841 ccgttaaagt tgcagattgt ccactacaaa tatttactgc tgtgcccggt gaaatggaaa
     9901 ttgaaggaac actgcctgca gtgatagtga ttgtgttgga tgctgtacca caagagctgg
     9961 taccggtaac tgtatatgtc cctgcagtac tgattgtgat tgatggcgta gtttgtccgc
    10021 ctgaccaaac ataggtgctt gcgcctgatg cagtaagcgt gatattttgt cccggacaaa
    10081 tttctgttcc gctgtttgaa gttatattta caacaggtgt tgtagtttgt gtaacattga
    10141 tagtcactga tgatgttcca caggcattgg acgaacttac agtataggtt cctgctgtgt
    10201 ttacagaaat tgatgatgtt gttgctccgg ttgaccattg ataagttgaa gcacctgaag
    10261 cagctaatgt tactgactga ccggcacaga tactgacatt cgttccaggt gtaacagtaa
    10321 tgttaggagc agtgcctcct gctatagttg ctgatgctgt aggtgttccg catgttcctg
    10381 tcaccgtaac tgaataagtt ccgggagtta aaacggtaat agattgtgtt gttgcaccgt
    10441 tgctccattg gtatgctgtt cctgatgatg cagtcaatgt tatactgtct ccggtacaaa
    10501 cagtagaagg acccgttatt gatgcaggct gaccattctg tacgattaag taaatattgc
    10561 ttgtaatagt gtcattgcat tgcccaatca tggaaaactg tatggtgtct gttcctgtaa
    10621 atgttgtatt ggtttgatag gttgtgacaa gattgttagg agaactcaaa gtgccattgc
    10681 caccgctcca aaagtatcct gaataatttc ctgaaagatt acttgctgtt aggataaccg
    10741 aatcaccgga gcaaactgtt gtagcactac tttgtaatgt agctgttagt gaaataatcg
    10801 gagcctgaca accattgtta taatatgttg catttcctaa agaatcatat cctacgcttg
    10861 atccatcctg tgctgcagtg tctaactgtc cgaattgatt tatgagtaag ttggggtaga
    10921 aagtaacaga ttggctacat actgctccaa aattcatagt taaaatacgt ggtgttgtaa
    10981 ccgtagcaga ataatttctg aaatgacctt gtgtgttccc aatacattga aaaatgacat
    11041 acatcgtatc agtcagattt gcaaaagagt tgaaggcagt attaaaattt tcactggtaa
    11101 ttaaaatgac cttactgttt tcaggtaaaa tatcatttga tggttcaagc aaataaccac
    11161 atgattgaat agttgcattc aaatcggcta ctttttgtgc tgttgtagca ttttgacaaa
    11221 tccccaaaaa gttaagactt gtattgggcc atgtaacatt tagcgaactt acctgtaatg
    11281 gagaagcgcc tacagtaaaa cgcaccattt cattttcacc ttcgggggtt ccacaggcat
    11341 ctacaagaat gctgttaatt gtaaaacagc tttggctata accagcagaa tacaataaaa
    11401 tactgataaa aaatagcaga aaattctttt tcattgatat aatatttatg atttaatttt
    11461 aaaattcaga agtgcaaaag tgcagaattt aactcatttc tgatgaaaca agagtcaaaa
    11521 atatattaag tttaataagg cattattttc ctttttaaag aatattttac cgtgtctttc
    11581 ttacccataa gcatggcatc ccatgtattc tgctctgaca gtgaaaatgt tatgcggtta
    11641 tagggtgtga ttttgtcatt atgaaaagtc atggaagtgg gattatgcac ttcggcttcc
    11701 cattgcagca ttttaagact gtcgctgtca ttgatgtaat aaaggcggtt gtttcttaat
    11761 gcaattctgg aagttccgcc aaaaacctgt ttatcacctt cgaaatgata gttattgttg
    11821 acaaatgcag tatctgattg ttttttccat tcttcataga atatgttacc gtttcttgct
    11881 gtgccttccc attttccgat taaccaatct aaatcgttaa gcttgttctt ctccttgcat
    11941 gaagatgtaa tcataaaaat tgatagaata agaaaataaa atttcttcat gctgcaaaaa
    12001 tgcagaaata atccaacttt aggtgcatta cttattaatt tttaacgttg attaagaaat
    12061 gcaataatta attttgcagc aattcagaaa gtgaaaccgg atagtatgtt gcaaactttt
    12121 tttagactta aaatgctgaa aaggcatttg agtttggctt gttttatagc ttttcttagt
    12181 agtgttaatc tatcctatgg acaggaaatg aatggtatag cacatagcaa tttcgcagga
    12241 aatatgggaa tagaaatgaa tcctgcttcc attgcaacaa tgccttatcg ctttgaattt
    12301 aatatactgt cgggagatat tttttttcag aatgattata tttactttcc ggcctcagac
    12361 attccaacag gtaagtttgc aaggtttgag ccattacgtc acgaagatta tagtgatcat
    12421 tatgataaaa caccgaagaa tgcttatggc aatttattgc ttaaaggtcc gtctgctgtt
    12481 tggcgtaatg aagaagcagc tttcggaatt catttttcat tacgcagcca gttaagcgca
    12541 ataaaagtac cttatcattt ggctaaattt ctttgggagg gaagtgacta tagcccacaa
    12601 cataaaattc cgtggaattc tacttccttc agtgcaggcg ctttggtgta tgccgaagcc
    12661 ggatttactt tcgctaaaac aattttaaca aaccgacaaa atattacaac agccggtggc
    12721 acagtcaatt ttttgttggg gatgagcggt acttatatca attcaactgt acttgattat
    12781 aattttacag atgcatcatt attgaatata aaaaatattg aagttgacta cggtcattca
    12841 attcctaatg atgataaaaa gaattatctg catccgcgag gtgcaggggc ttccatgagt
    12901 tttggcatac aacatttctt taattacatc aaccaggcat acaaacccgg catgtcagga
    12961 attaagttaa agaaatacga tctgaaactt gggctttctc tcattgatct cggcttcatt
    13021 aggtttaatg gtaatgcaag gacttttagt ctgaagaatg catctgctgt ttggcccgga
    13081 tttgatacca caaagattac aggctatgaa catgccgatt caatatttag tgcaaagctt
    13141 tatggcgatg ccaacatatc acggagtggc agtggtttta atatgatact tcctgctgct
    13201 gcatcatttc aggttgactg gagcattact ccaaaatatt atctcaatct ggcagtcatt
    13261 cagaatattc agatttttga cagaagtgta acacgtgctt cgcaagtttc ttttacacct
    13321 cgatttgaaa ccagaagagt agaggtgagt ttaccaatgt cattgtatca gtacaatttt
    13381 ttcagagcag gtattgctat tcgttataag tggtttgttc ttggtagtga taatcttggc
    13441 tggtggccgg gtttgtatga aatgaatgga cttgattttt attttggttt taaatatacc
    13501 agtgatattt acgataacat taggtttaaa aaaggtaaag gaggtcattg tccggcttac
    13561 aagtaggatg atgcatgatt aattcagcat acagcaattt tttttagtga tgatcaacat
    13621 cggctgacag aggcaaggag tgatgcgttg aaattgataa ggcaggatgc agcgtgcaac
    13681 attcagaata aatgattcag aataatttcg tgtacctttt aagtattttt gttttagtac
    13741 tgtcagataa aattttataa aaaataaagg agacagcaga acgttttctg ctgagctcct
    13801 ttaagggtgg gaataaaatc cccgtttttt ataaggtgcg tttaccatca acggtaacgg
    13861 tattgccgct gttgtcagta aatgtaccat caatatcatc accggaaagt gttgcaactt
    13921 tgatgttgtt atcatcaaat aaatctcctg atgatgtaat tgttccggaa acatcattgg
    13981 ttgctgtctg accattttca cgtgatattc caccccattg acctaatggt cttgagagta
    14041 gaatattaaa cgttcctgtc tccggctctg tagtagaata ggtgccttca aagccttcaa
    14101 ccaaggcatt tgaagtttct tttatgattg taaatactgc atttggatga cccggaatat
    14161 ttgatgaaat aatttgcggt gtacttccat tgttttgaac agagaaagta atactcactg
    14221 cttgtccgtt taatgtgcct gtaaaaggag caatgtaagg ctggccacta acccaactaa
    14281 ctgtagaggt tagtatagcc gaaacaccat caacaaccat ggttgctgta atgtcattcg
    14341 tgccattctg aatggaaaac tttatggtgc ctgaagagcc aatcaatatg cctttataaa
    14401 cacctttgct gcttccatcg ttggcagcta atgcttctgc ctgagttaca caactgctgc
    14461 acgtataatg aggattgtct ttgtctttct ttttgcatga agtaaaagtc aatgtggcaa
    14521 tgcataaaat gggaatgatt atctttttca ttttttctat tttttaattg taattaatga
    14581 ttaacggaaa gtgtatttgc ctaaccaaat gtttttgtaa tcctcatcat gtccgatgta
    14641 gaaacggttg ttatctttat ctgtcaggct ggtgatacta tgatagacta agtattttcc
    14701 tttttcaccc agctcggtaa agttgcttac tgagtgatta gaaagtgtga tattggcgat
    14761 gcgtggaaag taatgcgatg aaagagagta attgccgcta tatgctgccg ctactgaagc
    14821 ataatattta gctgcctgca cttctaaaat ttcccaatag acagatttac catctgcaga
    14881 tggaataaag ttttgaattg ccgaaaacac ttcgttttta ctatcatcta aaagtttgtt
    14941 caccgcatat tgtgctacta attttccctg attgtcaaat tgcatgcata catagtcgta
    15001 atatctgaat tcataggagt tgccaccatt cactactttt ttatcagaca actgacctgc
    15061 aaccagcaca tcaccggagg gtgcaacaaa aagattttca ataactattt tctttccggt
    15121 atagggttgg ccttttttct gtgaaggagg agtaaccatt tttgttttaa aatcttttac
    15181 cggctctgtg gaagcaaatg caagtttacc atcagagaat ctcaataagt gcaagtttgt
    15241 aaattcagtt tccatggcgc gtttttcgta tttgtccatt tgcctgttat tggaagttga
    15301 gtatccggga ttgtcaatag gagcataact ggtaaatacc ttattgtagg cctcattgct
    15361 tttgtcagat ccggcaatga aatacaagtc accgttagct tcgctataat ccattatcaa
    15421 catgttgggc gatggcgaag taaactcatt gctttttgca accttgctgt ctgcactaca
    15481 ttcaatgaaa taatattttt tggtgtcact cataccttca ttaggtgcaa atacagcaaa
    15541 aatgtttcct gatttcagtg taccacaata aaccaaggaa tattttcctt gtaacggcag
    15601 ttttgtttca gtcatgttta aacctttgtc aacgtgaaga caaacaaact gatcatcatc
    15661 accgctgtca tagctggcaa gtatcagcac gctgccatca tcgtcattgc cataagcagc
    15721 aaagcctctg tacttgctat cattattctt tggttttaca acttcttttg aaatacggct
    15781 gccccattga tatttttgtt tatcgaaatt ccagtttctt tcccagactt cctgttgcag
    15841 atttaaagac atactcatta cattaaatga gttgccacca ccaacaaatg cttttatcaa
    15901 agtaactttt tggtcaggct gtgtttgttt gtcttctttt gtcttttcaa ctccgatgaa
    15961 tttcagattt ttatcaaaga cataatcttc gtaattaacc tgatcactct ttttgtcaac
    16021 cttcattttg taagtcattc tgatgttgcc gtcagcactt agctcggcat ttgccagaat
    16081 acctttttga gcaggtttcg acaactcatg tgttgtttca ttgttttgtg caatgagagc
    16141 ggtcgggaag aggatggcag aagcaagcca tactgcggaa aattttggta acgtttgttt
    16201 cattttttgt ttttttttaa tgaaacaaaa ttagcccctg tgtaaaggtt agtcaatcac
    16261 catttagtag taattatttt ttgaatactt tgttaagcgc ttcagttctt gaatttacct
    16321 gaagtttgtg atagatattc cgaatgtgtg tgcggacggt ttctgtactt aaaaaaagtt
    16381 gatcggcaat ttccttatat ctcattcctt tagaaagtaa atcaacaatg agtcgttcgc
    16441 gttcagacaa tttttcatat tccggactta caggtgtttc cataaaactt gaaaccactt
    16501 tgcgtgcaat atggctgttc attggagaac cacctctgta aacatcattt atagcatcta
    16561 ataattttga tggttgtgtt gttttggtga ggtaacccat ggcaccggct ttcagtgctt
    16621 taaaaacatg ctcagaatca tcgaatgaag tacatatcaa aaaattggtt gacgggcatt
    16681 tgtcttttaa ttcaagaata caatctatgc cggtcttgcc tcctaactga atgtctgtca
    16741 gcactacatc aagttgcaaa gtaggtatga tatttatggc atcttctgca tttgagaata
    16801 aatgtttgca ggaaaagcct tcactgccat caattaatat ttgcagattc tctctaatgt
    16861 gcttttcgtc ttcaatgata ccaacctgaa ttgtcataag acaaatatag gtctttcatt
    16921 agcaaatgct atactactaa agtagtatat tcagattttt ttccaaagaa agtatagagc
    16981 cttcgcccgg ttttgaagat atatttagtg atgctccgat agttttactt ctctcagaca
    17041 tgttcctaag tccgtttcct gttgtctttt cattcatgct aaagccaatg ccattttcag
    17101 taattataag tttaaaaaca ggttgaattt ctatcctgat agatacctta gaagcgtttg
    17161 agtgtttaac aatgttctgt aaagattctt taactatcat cagaatattt ctgcttattt
    17221 caatatttat tttttctgaa ggtatatttt caggaaaggc atagcttact tctattggaa
    17281 agtcttctaa atactctgta gaaaattcgc gcacacgggc aatcagattg tctaatgttg
    17341 aattcatcgg attcatgttc caaatcatgt cgcgcatgtt gtcaatcaat tgatgcgatg
    17401 tacctgtaat tgtctgcaaa gtttctttta gctgaacttg gtctttggtt ttcatttcta
    17461 aaatttctga caggaatttg atttttgtta atcctgaacc caattcatca tgtatgtcct
    17521 ttacaatgcg tattcgttca ttttcttctg tttgtctgat tgtgttttgt tggtttatta
    17581 actcagcttg tttacgccat cgtaaataaa aataaatggc aatgacaatt gttgtgataa
    17641 gaatgagtaa gatgattact aaagcatttt gttttttaat attttccttt tgctctttca
    17701 gccggagctg gttaataaat gattgttgat taagaagttt gatagttgac tctttctttt
    17761 caagactgta acgtgcttcc atctctgcca gcctgttgta aaatgtaatt ggctgtatgc
    17821 tgtctttgat tctataagcc tgaagcagat tttccattgc aggctcatac tgtttaattg
    17881 atgcgtaaag ttcagaaagt gatttgtaac aatcgcgttc aataatttta ttgtgattgt
    17941 cttttgcaag tgttaatgct ttctgaatcc attcaatagc ttttctgttt tgaccaagct
    18001 caatatagat atcgccaagt tgctgatagg tgcctgcctc aatctccgga tagttacccg
    18061 gctgaataac ctcaagggcg tgtaatgcag tttcaagtgc ttgtttagtt tgattatgtt
    18121 caagataaat acggctcagg ttagagtaac atattgattt gctgcgttca tcacctaaag
    18181 ttgttgcaag tattagtgct tgctgatagc aatttgcagc actgtcatcg agcatttgtt
    18241 tgtagtaaga gtttccgttc tgaatcagag aagttatttg ccctgtcaga ttattcatta
    18301 gttcatattg aatgatactt gtttttattg tacttattga ttgttcgtaa agattttgcc
    18361 tgtaataaat tttacctaat acctcatgca accaaacttc tccttctttg cttctatttt
    18421 tctgatatat tttataagct ttaccataaa gatcaagtgc tttggaagga atattttctt
    18481 tgatgaatac ttctgatagt tgaaggcagg atttgaagag ttgtatggtt gtgtcattgg
    18541 atgaaaacga acttatactc tcattataat aatattgagc agagtctaat ttgccaatat
    18601 agaaatagca agcacctaat gttgatagta gctgttgtct tagttttaaa ttaattgatt
    18661 ttgatgaatg taatattttg tttagccctt caattgcaat attacttttg tttgtttgtg
    18721 attcaattat gctttgcaga caggaacttg caacaatacc ttctgtatat tcaatgttaa
    18781 ctgctttagt ttttgcatca actgcaattg tataagcacg tttaaagtca cttttgtaaa
    18841 ttatttcagc ttctgatatt cttgcatcaa tcagtttttg ttgtgctgta ggtgtagcgt
    18901 cagatgcata taatacatag tgagaaagca ataaaaaaac acaaaatatt attcttgaca
    18961 gaaaaggaga catgaaatta gaaatcgcat gctaaggtaa taaaacaata ttaccgtaag
    19021 tgagttatct tgtcaacaaa acactttaca ttcttaaaaa aaataaacta cctgtttgac
    19081 tgattgggaa tatcatcaag gtgcatctca aattgatctt ctttctcttt atacttcatt
    19141 atttgatcta atgcatcagg aataacatca ctgcagataa caataaatga gccttttttg
    19201 ccattgttaa tagcatggtc aatggcttcg cgttctaaag gtattacggt gacttttttg
    19261 ttggcatcta cagacttgat accttccagc ataaaatcaa tgatttcttt ttctgtccta
    19321 ccgcgaagat ttctgtcctg gcgaataatg atttcatcaa acatctttgc tgccaacgaa
    19381 cctaatgcta caatgtcttc atcgcgtctg tcgccaacac ctgcaatgac accaactttc
    19441 ggtttagcat caactttttc gaggaagcgg gcaatagcct gaaatcctgc agggttatgt
    19501 gcatagtcca ccatgaccgt aaatttcttg aattggaaca tgttcatacg acctggtgtt
    19561 tgtaccggag atggaataaa tgtttccaat gcaagtctaa tgtcttcaat tttaaaatta
    19621 cggataaaag cagaaagaat agcaggtaaa atattctgaa tcataaaaac tgctttaccg
    19681 gaaaaggtga gtggaatgtt gatgacttta tgtactctca gtttccaagt acctttacaa
    19741 atagttacaa atccattttc aagaattgca gcaaggccac cggcttcaca atgactttta
    19801 atgattgcat tgttttcatc aagactgaaa taagctacat tacaatccag attacgagcc
    19861 atagctgcta ccaaatcatc atcggcattt aaaatagcat aaccattaag ttgtacactt
    19921 tcaggtacca cggctttaac tttcgacatt tcttcaagcg tgttgatacc cttaagtcca
    19981 aggtgatctg cggcaacatt ggtaactacg gcaatatcac aacggtgaaa gccaagaccg
    20041 gcacgcagta tgccgcctct ggctgtctct aaaactgcaa aatcaacagt agggtctttt
    20101 aaaacaaact ctgcactttg tggtcctgta caatcgccac gctgcaacat ttgattctga
    20161 atgtaaatac catccgtggt agtgaaacct actttatgcc cacatgtctt agcaatgtgt
    20221 gctatcagac gtgtggtagt agttttacca ttggtgcctg tgacagcaat tattggaata
    20281 cgataattac ttccgggagg atagagcatg tcaacaacag gctctgcaac attgcgcggc
    20341 agcccatcag taggagcaat gtgcattctg aagccgggcg cagcattaac ttccagtacg
    20401 gcaccaccgg tttctgtcaa cggctcgctg atgtcgggag tcataatgtc tatgccgcaa
    20461 atgtcgagac caataattct tgcaatacgt tcgcacataa aaacattata tggatgaaca
    20521 atgtctgtga catctgttgc tgttccacct gtactcaggt ttgcagttcg tttcagaaac
    20581 aactcttcac cttttttaag aatgctgtca agtgtcagcc ctttttccac taaaatattc
    20641 tgtgtcatct catcaatctt tattgatgta agcacatttt catgtccata tcctctacgt
    20701 ggatctgcat taacggcatc aaccaattgt tgaatggtgc ttttaccatc gccaacaacc
    20761 gctgcgggtt tgcgctgtgc tgcacaaaca aatttatagt tgataactaa aatccgatga
    20821 tcataaccta ttatatactt ttcaacgatt acagagcgtg aaattttctt tgctatttct
    20881 aatgcctcta cagcctgatc ccaattgaca atattaattg ttgcgcctcg accatgatta
    20941 ccatttagtg gttttatgac taaaggatat cctaaatctt caacagcatc tttcaatcct
    21001 tcttcatcac gaattatttt gcctctgggg acagggatag actgagcttc aagcaggttt
    21061 ttggtatctt ctttatcaca ggcaatttct acagcaatgc tactggtagt gcttgcaatc
    21121 gtagcctgaa ttctgcgctg gttgatgcca taccccaatt gaactaaaga atgtcggttc
    21181 aagcgaatcc atggtatgcc tcttgatgct gcttcttcta caatagaacc ggtgctgggc
    21241 cccagacgtt cgtcttcgcg aatttcacga agttgttgta tgtcgttatt taaatcataa
    21301 gttttattgt ctataagagc ctgtgcaata cgaactgagg cacgtgcagc atatactccg
    21361 gccttttctt ctatgtaaga aaagacaaca tgataaactc catgctctcc ggtaccacga
    21421 gtacggccaa agcctgtatc catgccggca agcgtttgca attccaaagc aatatgctca
    21481 atcacatgtc ccatccaggt gccttcttta acacgcttaa aaaaacctcc ttcatgatct
    21541 tcgctgcatc tgtgcgattg catgctcgga aacatttttt tcaatctttc cagaaagcct
    21601 tcaattttgt cggttggtcg ctcttccagt tcttccagat ccaacttcat tactataagc
    21661 ttatgtctgc gtatgctcca aaagtttggt ccccgcatgg ggcgaatttc aagtatcctc
    21721 ataaaaaaac catttggttg tggcacgaat ata
//
LOCUS       DAOQNP010000054        21700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_822_length_21700_cov_8.250219, whole genome shotgun sequence.
ACCESSION   DAOQNP010000054 DAOQNP010000000
VERSION     DAOQNP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21700
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_822_length_21700_cov_8.250219"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /gene="priA"
                     /locus_tag="PKY09_08375"
     CDS             <1..1966
                     /gene="priA"
                     /locus_tag="PKY09_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNB12911.1"
                     /translation="LQKKLVLSVENIGEDFKIPMANFIRRTEQTNDQDFLKRQFDLLE
                     KKAPRQLDVLMQLLLLEKNYSEGKVPQKVLLHKSGVTAAVLKQLLKKGLIEILTERAT
                     GTKSFFNKDFFELSVEQSTALKEINNSFTEKPVTLLHGVTSSGKTELYIRLIDDCLKK
                     GRQALYLLPEIALTTQIITRLEKHFGNKLMVYHSRFSDNERASVYTRMIENGMASSEP
                     FVVIGARSSVFLPFHKLGLVIVDEEHEASYKQFDPSPRYHARDTAIVLAKLFEANVLL
                     GSATPSIETYHNAITGKYGLVELFARHAAIAPPQIQLVNIKELTFKRQMKSHFSPALI
                     NAIKEELDKGKQIILFQNRRGFAPILQCHQCNWVPHCQHCDVALTYYKKADLLRCHYC
                     GFTQQPPSKCKSCGETDIRQYGFGTEKIEDELQIFFPEHNIARLDLDSTRGKHAYSEI
                     ISRFETGETDILVGTQMVTKGLDFDNVSLVGILNADNLINFPDFRAYERSFQMLMQVS
                     GRAGRKKEQGKVIIQSYNPGHSLLDFVVKNDYKSFIDYELSERKKFNYPPFSRLLEIT
                     VKGRDESRTAQAANFLTSELKKQIPSILGPVTPPVSRIKNFYIRTILIKFQRQVKLSE
                     ARQRMLSAIDRYKINPVSKNILLQIDVDPI"
     gene            2183..3886
                     /locus_tag="PKY09_08380"
     CDS             2183..3886
                     /locus_tag="PKY09_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB12912.1"
                     /translation="MNKNKIIILSACMAIALIGIIAMQVNWILHDYHLKEQQFNQRVN
                     DALTAVAIKLEKKEAFNIFSNSFVTINNDSIFSLFRERKMLDQEWQGLDSTTENSIVN
                     RLAPETSIRPTPPMEPPLPPDFYNQDDYNNDFIKMEIKYSGGRKITIRHNRQEITLDD
                     SSKSNTHNAVKFLSLKADILEKQRAKIEAKINSWNKVMQNLAFEFISDEKTIEDRIKP
                     FQLDSILKTELSNRGITTPYQFGVINVGENKIMSTNSPENKNALLKSRYKTDLFPNDL
                     LAKPILLSLHFPQSLSYVLSTMWVMLISSSLFILVMLFGFSFTIYTIFRQKKLSDIKN
                     DFINNMTHEFKTPIATISLATDAVNSPAVSKNTESVHRYMNIIKEENQRMHKHVENIL
                     QLALFDRKHINLNLVEFDIHEMIQKAIEQIKLQVEVKGGKIETNLNAAQYHLLGDYDF
                     IFNSIINLLDNAIKYTPANPIISIATENIQNNIRLLISDNGIGMSREVQKNIFEKFYR
                     AGTGNIHDVKGYGIGLSIVKAIAHAHNGEIKVLKSIKGVGTTMELLLPLNLHKAKRKS
                     N"
     gene            3921..4628
                     /locus_tag="PKY09_08385"
     CDS             3921..4628
                     /locus_tag="PKY09_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB12913.1"
                     /translation="MSILTGKTKIMIAEDDTNFGLVLKDYLSLHEYDVHLYRDGRQAF
                     EKFEKGKFNLCILDVMMPIMDGFALAENIKAKDSNVPIIFLTAKSMKVDMIRGFKIGA
                     DDYITKPFDSEILLFKIKALLNRSETIVKAVNEQVEFVIGGFKFNSRLRTIQGFGKEE
                     KLSPKEAALLALLCVYLNDILPREVALRKIWNDDNYFTARSMDVFITKIRKYLKDDPN
                     IELLNVHGNGYRLVVKD"
     gene            4746..6698
                     /locus_tag="PKY09_08390"
     CDS             4746..6698
                     /locus_tag="PKY09_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB12914.1"
                     /translation="MLRLFLAMLLFYPQLASSDDLNNKINLYLRDTHYIQSADYITSI
                     INHYLVDDVASDSAIMWSDTLRNLGEAKGDLELVAKSDVLTGKYYLRIANDAEALDYF
                     YRALKYYKSVGNIDGEADIYIQIGLVYYLQGQFPDALPNIKLATELYQKAKNDSSLST
                     SYYLTGLSYLEIKSYKKAEYFLQQSLRLGKLLSNNQREYECYVALARLYTSQKNYVAA
                     EQLLDKVLTKWDSIIDPYGYCMALIPYSKLLIETNKKEAAFINLTLALSIADKFNFIR
                     FKVDIYKLLAIIYTDKKNYESANYYLLQHFQLRDSIYNSENQRAINTLKAKFEAEKKQ
                     AQLDSLFQEEKADKLQKWLLALTILMLLGLSFGLYRRYHYKQKSEAELRIANRELSEA
                     LNSLRQVQQQLIHNEKMASLGRMSSGIAHELRNPLNFVINFTKISSELIADIDKKIQN
                     NHDEDFEQLKNNLAMIYQHSLRADKIILNFANHAKPKSNSRTIEDLNKVVNEFTSVAY
                     HSFITHYPDFHCHIHYDFDQSLKGIVLNLQEITTVLLNLLNNAFDAMNEKLMVDRTYK
                     PILKIQTRQILNSVRLTITDNGPGIEEENLPRVYEPFFTTKTLGKGTGLGLSLSYDII
                     KSHNGDMDVKSKPHLETEFSFTLPYN"
     gene            6727..7206
                     /locus_tag="PKY09_08395"
     CDS             6727..7206
                     /locus_tag="PKY09_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12915.1"
                     /translation="MKVTGYVLLASLAINAVFAFNNFFVSNAEANNKSAGIEAPPCAC
                     DDALRYDKVEKEGGGEWISTENGKAAINKFYSTYKTAYKGAFFSKRALDEIFCKDNKA
                     NGIVCYFGQTGEKDTTVTLLIEGTHSETTLIKRGEQNAPTAFIATVKCPVICSPIGQ"
     gene            complement(7211..7723)
                     /locus_tag="PKY09_08400"
     CDS             complement(7211..7723)
                     /locus_tag="PKY09_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12916.1"
                     /translation="MKERIFIILIVCFFWSCTCSVVHDEPTESAQQAKILKNDYAAAP
                     QYIQVDAAASVNNTSWKPVTGVVNVSVNPDGKKNYAIMLTGDGITVALTYNGKEKNGN
                     YKIGEGFSASVLNAKSVVYNCNDGYIRFSEFDTTNKVLSGKFAISCMENKASGNGFDI
                     QEASFNQLKW"
     gene            complement(7778..8419)
                     /locus_tag="PKY09_08405"
     CDS             complement(7778..8419)
                     /locus_tag="PKY09_08405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNB12917.1"
                     /translation="MHFISEDLEEYCLKHTSKESNLLEQIDRETSAKVLNPRMLSGHL
                     QGRFLAMISRMINPTRILEIGTYTGYSALCLAEGLAEGGKITTIEKNAELENMIRKNI
                     NQSIYAGKIELHIGNALTIIPELNEQYQLVFIDADKENYSIYFEMVWEKLSSGGFVLA
                     DNVLWSGKVIEPTVAGDMDTERIKMFNEMVVSKADAECVLLPVRDGLMLIRKR"
     gene            8494..8886
                     /locus_tag="PKY09_08410"
     CDS             8494..8886
                     /locus_tag="PKY09_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12918.1"
                     /translation="MFRKGIRFILSFVLIFSVLASASGITIFKMVCTKKNKTLVSLDQ
                     FNHCSNKENKECSFKRDCCQFSKSVIDANQLSNHENKVQVSTPAIVELPQLLENTSLS
                     SVFLLNFSIQEQTIPFPPLLLLISKLSI"
     gene            8941..11142
                     /locus_tag="PKY09_08415"
     CDS             8941..11142
                     /locus_tag="PKY09_08415"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB12919.1"
                     /translation="MKKTISFIILLVISLQSHAQIIIRGVVKSDNSETLPGVSVYIPI
                     TDQHTVTDVDGKFQIQYTGTTPLTIISQFVGFKTDTLKLNAIPDNDLKIRLKKSIDLK
                     EVEIKSRRESTSISTITPINTTTITQHELLKAACCNLSESFETNPTIDVNYSDAVTGA
                     KQIQMLGLDGIYSQIQFQNLPLIFGLSASHGLAFTPGPWIESIQINKGIGSVINGFEA
                     MTGQINIEMKKPQNADRFFINGYLNNEGREELNLQLAQRFKKHISTVFLAHGSMNHLK
                     VDRDKDGFLDQPLTKQVNAMNVWHITVPNKLEGEIGWHALYENRLGGQKGFNYDDDFG
                     KSTLYGVGIENKMFEVYTKTGTINPAKPYKSIALLTLSRLHLQDNYYGNKTYIGDQRS
                     FHANTIYQTIIHDTRNYIRSGLSFRYNHYSEKYNSVGTYTNEIIPGAYAEYSFNNDLN
                     WAVVAGLRADVHNTYGFFLTPRLHIKYNIDQLTAIRLSAGKGWRTARVYAENTSMFTS
                     SRALFIDDLKAEEAWNTGINFTKKMQWFGHDASFNADYYYTWFKNQVVIDMEDIHQLR
                     FYNLDGKSFSHYLQGELNIEPINNLLLRFGYKYNVVKQTFSGELKSKPLTPRDKALLN
                     AEYKIKKGKYSIDATLKYTGKGRIPGGGVQMNGYEVKSTSESFITLNSQATWRTKHAD
                     FYLGCENITDFRQKHAIIAADEPFGQNFDASMIWGPLMGRTFYVGFRYTIK"
     gene            11152..11496
                     /locus_tag="PKY09_08420"
     CDS             11152..11496
                     /locus_tag="PKY09_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNB12920.1"
                     /translation="MKKLITGAVMLSLFSFSLMAQQNKKAEINIATSAECTMCKKALE
                     TALLHQHGVKFAKLDLNTRVVAIKYNGEKITPETIRQVISNAGYDADSIKANPEAFEK
                     LPDCCKNGALHH"
     gene            complement(11768..11920)
                     /locus_tag="PKY09_08425"
     CDS             complement(11768..11920)
                     /locus_tag="PKY09_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12921.1"
                     /translation="MGIMMPVMDVVEVCRVVHTMPALNKTIKTLSRNANELSVVDATI
                     AVIGGL"
     gene            12052..12831
                     /locus_tag="PKY09_08430"
     CDS             12052..12831
                     /locus_tag="PKY09_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB12922.1"
                     /translation="MGSTVLQEINGQVVILTLNRPEKFNSFNREMSLQLQDELKKAAN
                     NSEIRAILISGTGKAFCAGQDLSEAIDANGPGIDAIVREHYNPIITLIRNIEKPIVCA
                     VNGVAAGAGANIALCCDIVLAAESALFIQAFSKIGLIPDSGGTFFLPRLIGFQRASAL
                     TMLGDKVSAAEAVSMGMIYKSVEDTLLMDTAMQISQTLANLPTKALGLTKKLLNQSLS
                     NDLNTQLECELEEQVSAAKSYDHSEGIKAFLEKRKPVFKGQ"
     gene            12828..13988
                     /locus_tag="PKY09_08435"
     CDS             12828..13988
                     /locus_tag="PKY09_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNB12923.1"
                     /translation="MKQHVGIAGAGSMGSGIAQVAATNGNPVTLFDTNSEALQKAQSS
                     IQSSLDKLVEKNKIDKTQSNFIFSQIKFTDNINELKSSDLFIEAVIENIEIKQSLFKN
                     VEGIVSDNCILATNTSSLPVIAIGAACNKQDRIIGLHFFNPPVLMHLVEVIPSFNTSE
                     KVLTDSVEIIRQWGKTPVIAKDTPGFIVNRIARPYYGEALRIYEEGIADFATIDYAMR
                     NIGGFRMGPFELMDFIGNDVNFKVTETVFTQMFFDPRYKPSITQKRLYEGKLFGRKSN
                     RGYYDYKNSNQPQPSLDGNLHNQIFDRIFAMLVNEAADALMMGIATAADIETAMTKGV
                     NYPQGLLAWANKRGLRTVYNKLDDLFAYYGEDRYRVCPLLRKLALNDEMFRI"
     gene            14067..15341
                     /locus_tag="PKY09_08440"
     CDS             14067..15341
                     /locus_tag="PKY09_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB12924.1"
                     /translation="MENQEQKSKFQQAIDVITGVAKITIGAGIVHCKAQDEQMDGRYI
                     TLEGQKKLFFGTCGYLGIEKHPKIIEAAVEALHSHGAQFSSSRAYVSSHYYEEAESLF
                     SKMFGRPALVMQSLTLGHITGLPLLVGDNDAIIMDVQAHDSIQSATAMLKLRNIKIDI
                     VRHNRMDILEDRIKVLKNRYQKIWYLGDGVYSMHGDFAPVKELYALADKYEQLHLYLD
                     DIHGMSWTGPHGTGMVMNAVPYYHRKLFLSTGMTKAFGTAGGLLTYPDEEYFKLVKTC
                     GKGFIFSIQLPPAILAATIASCKIHMSDEIIPLQQSLMAKIDYFNKKAEELGLLVIRK
                     ERSPVFFIGVGNPNVGYSTVLRMKKAGFFFNLSVFPAVSIKHTGLRITLSNHIQMEDI
                     DELLETLAENLPVVFKENNTSFEQIRKEFANH"
     gene            complement(15338..16399)
                     /locus_tag="PKY09_08445"
     CDS             complement(15338..16399)
                     /locus_tag="PKY09_08445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB12925.1"
                     /translation="MDHQTRYIPHLDGIRFIAAMMVVLNHYLAELNFDYKFSIGFYGV
                     QIFFAISGFLITGILLRLKNAEKVISRRLIIKNFFAKRFLRLFPPYYLLIFSYIFLEC
                     FGLYIAQKGWIVFYLAYLPNWFFYFHGWQGTIDNHLWSLGVEEQFYLLWPFVLLLLPK
                     GKEFKWVITMIITGYLFKCIIRQYFMLRGDPFLLPISQMDTLGIGALLACGFEYRMRF
                     IEYFKKALPYLLPLAILLTLVTSYDKPRNDPFLCLSLILLSSSLVLNAVIGFKGVVKT
                     FMENKWVVYGGKISYGIYLYHKVIPTLFISSVERAGYEMPNRWLTMMVSIALTFLISH
                     LSWKLIESPILKLKKNFEH"
     gene            complement(16542..16973)
                     /locus_tag="PKY09_08450"
     CDS             complement(16542..16973)
                     /locus_tag="PKY09_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB12926.1"
                     /translation="MNKQLTILLIDDDKVDSMIIKRAIAKLDFPYHVDTAHNGLDALM
                     MLRGMSDIRIKRPDIILLDINMPLMNGLEFLSELRADPDLKDIHVCVLTTSEDDSDVK
                     TAYEFNVAGYFIKPLNISDFDDRFLYLNGYWQRNCFPVQKV"
     gene            17082..18863
                     /locus_tag="PKY09_08455"
     CDS             17082..18863
                     /locus_tag="PKY09_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB12927.1"
                     /translation="MDTPVVKWIIRKLMLDSIYRSIQDDDILAKQRFNLFRTFNLVAI
                     IGIGLICYQAIYVFQTQKNLSFILLGLELLLILNYLLLNIHKKLTLSVVISLLSCFGA
                     LHYITYFAGGIRNSGMFYQIAFILISFMLLGSRYGIWFTVLSVANMIYFFLITEYTNW
                     VDYSFIGETASLINQDYLITSLVSLIVISSLINNLESKKNVVISTITDSRNKLTIINK
                     ELKKLSLVASKTDNAVVITNENGVIEWVNEGFTRITGYQFYDAKDQSMPAFLSGPETD
                     KENLQKVQNSMREFKFCDSELLRYKKDGATVWLHASITPISDNEKSSRKFIFIMSDVN
                     ERKKAEIQLNEYLSDLEKTNKELDKFAYIVSHDLKAPLRAIGNLTDWITQDSADSLSD
                     ESKENFQIIKGRVKRMEQLINAILEYSKVSKHKGTQELFSFNEIIHNAVDLAGTDSNC
                     KVEISGTMPEYYGDKVKYQQVFMNLISNGIKHNNKEQKKIEISCREEGKFWEFMIKDN
                     GPGIDKRFHEKVFVIFQTLKARDEFESTGIGLSIVKKIVEEAGGSIRIESTPGEGAAF
                     YFTVPKNQIDASLITGKNNQQISPIVL"
     gene            18930..19280
                     /locus_tag="PKY09_08460"
     CDS             18930..19280
                     /locus_tag="PKY09_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12928.1"
                     /translation="MKQKQKKSNNLSKVKNFDFDQYLNLRKAERIKLLQQMVEKLDPA
                     SRKNFFKSLLQYVLSDEELTALAFKNNSGKQEVKKSRVVTLSDKEKEMLGQIADYFHE
                     LELIMDLDELLINP"
     gene            complement(19295..19780)
                     /locus_tag="PKY09_08465"
     CDS             complement(19295..19780)
                     /locus_tag="PKY09_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB12929.1"
                     /translation="MLKFLSIAQSKRRENKQILEKISKSKSPHLDHFFREESEGFFKE
                     FSCLDCANCCKTTSPIFRDVDIERIATYLKLKPGDFVAKYLYLDSDGHYVLSYSPCPF
                     LGKDNYCSIYSVRPQACREYPHTDRKNIKQIMDLTYQNTLVCPAVASIVEKLKTDFIK
                     K"
     gene            19961..20464
                     /gene="sixA"
                     /locus_tag="PKY09_08470"
     CDS             19961..20464
                     /gene="sixA"
                     /locus_tag="PKY09_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNB12930.1"
                     /translation="MPKTIFLIRHSKATREYSQINDSDRPLTEKGCHDTEKMSLILKD
                     HTNSVDLILTSTAVRAYSSALIYCKHLDYHPNNIKLEKNLYMTGSKDMLAILKKLDEK
                     INSVIIIAHNPGIERLAKLLTGIESLHFTTSGIAMIDSKIKNWSELDSGSCNLLSYRH
                     PKETIYN"
     gene            20572..>21700
                     /locus_tag="PKY09_08475"
     CDS             20572..>21700
                     /locus_tag="PKY09_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNB12931.1"
                     /translation="MKRKIEQVAVLGSGIMGSRIACHFANAGYKVMLLDIAPKEFTDI
                     DKKAGFTEDSLKFRNKITDAHLQTAVKSNPSPLYHKSFAQRISTGNFTDNLKDIANCD
                     WIIEVIIEDLNIKRTLFEQVEKFRKPGTLITSNTSGIPIHLMTEGRSDDFKKHFCGTH
                     FFNPPRYLPLLEIIPTQFTDKDVIDFISHFGDRYLGKTTVLCKDTPAFIANRIGVFGI
                     MSLFHSMEKTGLSLSAIDKLTGPIIGRPKSATFRTSDVVGLDTLVHVANDAYKNLPND
                     EQRNLFAIPPFINKMIENKWLGDKTKQGFYKKTKSAEGKTEILELDLKSLEYKPSTKV
                     KFKTIESTKTVDDLKQRLPMLLGGTDEAAAFYRSMFFSLFTY"
BASE COUNT         7618 a         3829 c         3654 g         6599 t
ORIGIN      
        1 acttcagaaa aaactggtac tatccgttga aaacattggt gaagatttta aaatacctat
       61 ggcaaacttc attaggagaa cagaacaaac taatgatcag gattttttaa aaagacaatt
      121 tgacctcctc gaaaaaaaag caccaagaca attagatgtg ttgatgcaat tacttctgtt
      181 agagaagaat tatagcgaag gtaaagttcc acaaaaagtg ctgctccata aatcaggagt
      241 aactgcagca gtattaaaac aactactaaa aaaagggttg attgaaattc ttactgaaag
      301 agcaacaggc acaaagtcat tttttaataa agatttcttt gaacttagcg tagaacaatc
      361 aactgcatta aaagaaatca ataattcttt tacagaaaag cctgtaaccc tactacatgg
      421 tgtaacatca tcaggtaaaa cagagttgta tatcaggctt attgacgatt gcctgaaaaa
      481 aggaaggcag gcactatacc ttcttcccga aattgcattg acaacacaaa tcatcacaag
      541 gcttgagaaa catttcggca ataaattaat ggtttaccac tcacgattca gtgataatga
      601 aagagcatct gtctatacaa gaatgataga aaatggcatg gcaagttcag agccatttgt
      661 tgttattggt gcacgttcat ctgttttttt accttttcat aaattaggac ttgtcatagt
      721 ggacgaagaa catgaagcat cctataagca attcgatccg tcaccacgtt atcatgcacg
      781 cgacacagca attgtattag caaaactttt tgaggctaac gtattattag gcagtgctac
      841 accgtcaatt gaaacatacc acaatgccat caccggaaaa tatggattgg ttgaactgtt
      901 tgcaagacat gcagctatag cacctccaca aattcaattg gtgaatatta aagaactcac
      961 ttttaaaaga cagatgaaat cgcatttttc accagcgttg attaatgcca taaaagaaga
     1021 attagataaa ggaaaacaaa ttatattatt tcaaaacaga cgtggctttg ctcctattct
     1081 gcaatgtcat caatgcaatt gggttccaca ttgtcaacat tgtgatgtag ctttaaccta
     1141 ttataaaaaa gctgatctgc tacgttgtca ttattgtggt tttacacaac aaccgccatc
     1201 aaaatgtaag tcctgtggtg aaacagatat ccgtcaatat gggtttggca ctgaaaaaat
     1261 agaagatgaa ttacaaatat tttttccgga acacaatatt gcacgtttag accttgatag
     1321 cacaagaggt aagcatgctt acagtgagat aatcagccgt tttgaaaccg gtgagacaga
     1381 tattttggta ggtacacaaa tggttacaaa aggattggac ttcgataatg ttagtttggt
     1441 tggtattcta aatgcagaca atcttatcaa cttccctgat ttcagggcct atgaacgaag
     1501 ttttcaaatg ttaatgcaag taagtggtcg tgccggacga aaaaaagaac agggaaaagt
     1561 aatcattcaa tcttacaatc ccggacattc attacttgac tttgtagtta aaaatgatta
     1621 taaatcattt attgactatg aattgtctga gcgcaagaaa ttcaactatc caccctttag
     1681 cagactattg gaaattaccg ttaaaggccg agatgaatca agaaccgcac aggcagcaaa
     1741 ttttttgacc tcagaactta agaaacaaat accctcaatt cttggcccgg taacaccccc
     1801 tgtttcaaga ataaaaaact tttacatcag aactatactc ataaaatttc agcgtcaggt
     1861 taaattaagt gaagcaagac aaaggatgct ttcagcaata gatcgttata agataaaccc
     1921 tgtatctaag aacatccttt tacaaattga tgtagatccg atttgaagat tttttattgt
     1981 caaaagctac aattttccga gacaatcaat ttcaatatat aaggttacct ctacacccaa
     2041 tcaatttgct atactttttg atttttcata accagtaaat tttaaaacaa cggaatcaat
     2101 catcaatcaa tagcacatta aacagggtta aaataagtta aacagcattt tgctgtgtta
     2161 aagaatttta aatttgcata taatgaataa gaataagatc atcatacttt ctgcttgtat
     2221 ggcaatagca ctcataggca tcattgctat gcaggtaaac tggattttgc atgactatca
     2281 tcttaaagag caacaattca atcagagagt aaatgatgct ttgactgcag tagcaattaa
     2341 acttgaaaaa aaagaagcat ttaatatttt cagcaatagt tttgtaacaa tcaataacga
     2401 ttcaattttt tcgcttttca gagaacgtaa aatgctggat caggaatggc aaggattgga
     2461 ttcaacaaca gaaaactcca ttgtaaatag acttgctccg gaaacctcaa ttcgacctac
     2521 gccccctatg gagccaccat tgcctcctga cttttataat caggatgact acaacaatga
     2581 ctttattaaa atggaaatta aatatagtgg cggcagaaaa attacaataa ggcataatcg
     2641 tcaggaaata accttagacg attcttcaaa atccaacaca cacaatgcag ttaaatttct
     2701 atcgcttaaa gccgatatac tggaaaaaca acgtgcaaaa attgaagcta aaattaacag
     2761 ctggaataaa gtgatgcaga atctggcttt tgaatttatc tcagatgaaa aaactattga
     2821 agacagaatt aaaccttttc aacttgattc aatcttaaaa acagaacttt caaatagagg
     2881 aataacaacg ccatatcaat ttggtgttat caatgttggc gaaaacaaaa tcatgtcaac
     2941 caactcgcct gaaaacaaaa atgcactatt aaaaagtcgt tataagacag atttgttccc
     3001 caacgactta cttgctaaac ctattttact ctcactccat tttccacaaa gtttatcgta
     3061 tgtactttcc acaatgtggg tcatgcttat tagttcgtca ctatttattt tagtgatgtt
     3121 atttgggttt agttttacaa tatacactat tttccgacag aaaaaattgt ctgatattaa
     3181 aaatgatttt atcaataaca tgactcatga attcaagaca ccaattgcaa caatttcatt
     3241 agcaaccgat gctgtgaaca gccctgctgt ttcaaaaaat actgaaagtg tacatcgcta
     3301 tatgaatatt attaaagaag aaaatcaaag aatgcacaaa catgtagaaa atatactgca
     3361 actggcattg tttgaccgaa agcatatcaa tctcaacctg gttgaatttg atatacacga
     3421 aatgatacaa aaggctattg aacagataaa actacaggtt gaagtaaaag gaggcaaaat
     3481 agaaaccaat ttgaatgcag ctcaatatca tctgttaggc gattatgatt ttatttttaa
     3541 ttcaatcatc aaccttcttg ataatgccat taaatacact cctgcaaacc ctataatttc
     3601 tatcgctaca gaaaatattc aaaataacat tcgcttactt atttctgata atgggattgg
     3661 gatgagtcgt gaggttcaga aaaatatttt tgaaaaattt tatcgggcag gaacaggtaa
     3721 tatacatgat gttaaaggtt atggcatagg gttatcaatt gtaaaagcaa ttgcacatgc
     3781 acataatgga gaaataaaag ttttaaaaag tataaaaggt gtaggaacaa caatggaatt
     3841 attattacca ttaaatctgc ataaagcaaa aagaaaaagc aattgataaa tagattacaa
     3901 caaaacaaca taacaaaaac atgtcaatac taactggaaa aaccaaaatt atgatagctg
     3961 aagatgacac caacttcggc ttagtactta aagactatct ttcattacat gaatatgatg
     4021 ttcacctata cagagatggc agacaagctt ttgaaaaatt tgaaaaaggg aaattcaatc
     4081 tatgcatact ggatgtaatg atgcctataa tggatggctt tgctttagcc gaaaacatta
     4141 aagccaaaga cagcaatgtg ccaataatct tcttaacagc caagtcaatg aaagtagaca
     4201 tgattagagg atttaaaatt ggtgctgatg attacatcac caaacctttt gactctgaaa
     4261 ttctgttatt caaaattaaa gcattgctca acagaagtga gacgatagta aaagcagtta
     4321 acgagcaagt agaatttgtc ataggggggt ttaaattcaa cagcagatta agaacaatac
     4381 agggattcgg aaaagaagaa aaactttcac ctaaggaagc tgccctactt gctttactgt
     4441 gtgtctatct taatgacata ttaccgcgtg aagtagcatt acgcaagata tggaatgacg
     4501 acaattactt tacagctcgc agtatggacg tgtttattac caaaatcagg aagtatctga
     4561 aagatgatcc aaacattgaa ttattgaatg ttcatggcaa tggttatcgt cttgtagtga
     4621 aagattgaca ctttgtacaa ttttttatgt aaatgtaatc taagttgtta tctttgggga
     4681 caataaccct accgggtatg cctaaagata aacaatgcgc tagtactatc agtctgtgtt
     4741 ataaaatgct tagacttttt ttggcaatgc ttttgtttta tccacaactt gcctcctcag
     4801 acgatttaaa taataagata aatctttatt tgagagatac acattatata caatcggcag
     4861 attatataac ttcaattatt aatcattatt tagttgatga tgtagcttcc gattcagcaa
     4921 tcatgtggtc tgacactttg cgtaatctgg gtgaagctaa aggtgatctt gagttggtgg
     4981 caaaaagtga tgtgttgaca ggaaaatact atctcagaat agcaaacgat gctgaagcat
     5041 tagattattt ttacagagca ctgaaatact acaaaagtgt tggcaatatt gatggtgaag
     5101 cagatatata tatccaaatt ggattggtct attatctgca aggtcaattt cctgatgcac
     5161 ttcccaatat caaactagca acagaacttt atcaaaaagc aaagaacgac agctcacttt
     5221 ctacctcata ctatcttacc ggtttaagtt atcttgaaat aaaaagttat aaaaaagcag
     5281 aatattttct tcaacaatca ttaagactag gcaaactttt atcaaacaac cagagagaat
     5341 atgaatgtta tgtagcgtta gcaagactat acactagtca gaagaactat gtagcagctg
     5401 aacaactgct tgataaggta ttgacaaaat gggattccat aattgatcct tatggttatt
     5461 gcatggctct cattccgtat agtaaattac taattgaaac taataaaaaa gaagctgctt
     5521 ttataaatct gactcttgca ctatccattg ctgataaatt taacttcatc agatttaagg
     5581 tagatattta taagttactc gcaataattt acactgataa aaaaaactat gagtcagcaa
     5641 actactattt gcttcaacat ttccaattac gcgactctat ttataattca gaaaaccaac
     5701 gtgcaatcaa taccttaaaa gcaaaatttg aagctgaaaa aaagcaggct cagcttgata
     5761 gtttatttca agaagaaaaa gccgataaac ttcagaagtg gttgctggca ttgactattt
     5821 taatgttatt gggtctttca ttcggattat acagaagata tcattataag caaaaaagcg
     5881 aagcggaatt acgaattgcc aatagagagt tgtctgaagc attaaatagt ttaagacaag
     5941 tacaacaaca actcatacac aatgaaaaaa tggcatcgtt agggcgcatg tcaagcggca
     6001 ttgcacacga acttcgcaat ccattaaatt ttgtcattaa tttcactaaa atttcgagtg
     6061 aattaattgc agatattgat aagaaaattc aaaataatca tgatgaagat tttgagcagt
     6121 taaagaataa tcttgccatg atatatcaac attcattaag ggcagataaa ataattctca
     6181 actttgcaaa tcatgcaaag ccaaaaagta atagcagaac tattgaagac cttaacaagg
     6241 ttgtaaatga atttaccagt gttgcatatc attcgtttat tacacattac cctgacttcc
     6301 attgtcacat tcattacgac tttgaccaat cgctcaaagg catagtttta aatcttcagg
     6361 aaatcacaac tgttttattg aacttattaa acaatgcatt tgatgcaatg aatgaaaaat
     6421 taatggttga taggacatac aaaccaatac ttaaaattca aacacgtcaa attctaaatt
     6481 cagtaagact aaccattaca gataatggtc ctggtattga agaagaaaac ttgcctcggg
     6541 tttacgaacc tttttttacc acaaaaacac tgggcaaagg aaccggttta ggcctaagtc
     6601 tctcatacga tataattaaa tcgcataatg gagacatgga tgttaaatca aaaccacatt
     6661 tagaaacaga attctcattt acattaccat ataattaaat caaaaatcaa tttaaaatca
     6721 aaaattatga aagtaacagg ttacgttttg ctggcatcgc tggcaattaa tgcagtattt
     6781 gcattcaaca atttctttgt aagcaatgct gaagcaaaca ataaatcagc cggtattgag
     6841 gcaccacctt gtgcatgtga tgacgcattg cggtacgata aagttgaaaa ggaaggcgga
     6901 ggtgaatgga tttctacaga aaacggaaag gctgctatta ataagtttta tagcacttat
     6961 aaaacagctt acaaaggtgc attcttcagt aaacgtgcat tggatgaaat tttttgtaaa
     7021 gacaataaag caaatggcat tgtatgctac tttggtcaaa cgggagagaa agacacaaca
     7081 gtgactttat tgattgaggg tacacattct gaaaccacat taattaaacg tggtgaacaa
     7141 aatgctccta cagcctttat tgcaacagta aagtgtcctg taatttgtag tccgattggt
     7201 caatgatcaa ctaccatttt aattgattaa acgaggcttc ctgaatgtca aaaccattcc
     7261 cggaagcctt gttttccata catgatatcg cgaatttacc tgatagcact ttatttgtag
     7321 tatcaaattc tgaaaacctg atatagccat cattacaatt atatacaaca gactttgcat
     7381 taaggactga tgcactaaac ccttcaccta ttttataatt gccattcttt tcttttccat
     7441 tataagttaa tgcgacagta attccatcac ctgtcaacat gattgcataa ttcttcttcc
     7501 catcaggatt aacagacaca ttaactactc ctgtaacagg cttccaactt gtgttgttta
     7561 cacttgctgc tgcatccact tgaatatact gaggcgctgc ggcataatcg ttttttaaaa
     7621 tcttagcttg ttgagcactt tctgttggct catcgtgaac tacagaacat gtgcagctcc
     7681 agaaaaagca aacaattagt ataataaaaa ttctttcttt catcctaaac atatttttgt
     7741 tgaacaaaga tgtacttttt gggttgaaat aacacaatta tcttttcctg attagcatta
     7801 atccatctct cacaggaagt aaaacacatt ctgcatcagc cttactcacc accatttcgt
     7861 taaacatttt tattctttct gtatccatgt cacctgcaac agttggttca attactttgc
     7921 cactccaaag aacattatca gccaacacaa aaccacccga agacaacttc tcccaaacca
     7981 tttcaaaata aattgaataa ttttctttgt ctgcatcaat aaataccaat tggtattgct
     8041 catttaattc gggaataata gttaatgcat taccaatatg taactcaatt ttgccggcat
     8101 agatactttg atttatattt tttcgaatca tattttcaag ctcagcattt ttttcaattg
     8161 ttgtgatctt tcctccttca gctaaacctt ctgccaagca taatgctgag tacccggtat
     8221 aagtaccaat ttccagaatt cgggttgggt ttatcattct actgatcatt gccaaaaatc
     8281 ttccctgtaa atgtcccgaa agcatacggg gattaagtac tttagcagag gtttcacgat
     8341 ctatctgttc taacaaatta ctctctttgg aagtatgctt taggcagtat tcttctaaat
     8401 cttcactgat aaaatgcatg ttatatttta ctgcgaaagt aatatttacc ccaattcatt
     8461 gccatttcag aaagttgtta ctatttttgc ttcatgttta gaaaaggcat acgcttcatt
     8521 ctttcatttg ttttaatctt ttccgtactg gcatcagctt cgggtataac tatatttaaa
     8581 atggtttgta caaaaaagaa taaaacttta gtatcactcg atcaattcaa tcactgttcg
     8641 aacaaagaaa ataaagagtg tagttttaag agagattgct gccagttttc aaaatcagta
     8701 attgatgcca accaactttc taatcacgaa aacaaagtac aggtttcaac gcctgccatt
     8761 gttgaattac cccaactttt agagaacaca agcctatcct ctgttttcct gcttaatttt
     8821 tcaattcagg aacaaacaat acccttccct cctttactcc ttctgatttc taaattatct
     8881 atttgattca ggctattttt ttaaatagct tatttattaa ataatttcaa tcagatttca
     8941 atgaaaaaaa ctatatcatt tataatatta cttgtaatat ctttacaaag tcatgcccaa
     9001 ataataataa gaggcgtggt aaaatcagac aatagtgaaa cgctacccgg agttagtgta
     9061 tatattccaa taaccgatca acatacagta acagatgttg atggaaaatt tcaaatacaa
     9121 tacaccggta caacaccact aaccatcatc agtcagtttg ttggttttaa gaccgataca
     9181 ttgaaattaa atgcaatacc tgataacgat ttaaaaattc gtctgaaaaa gtctattgac
     9241 ttaaaagaag ttgaaattaa gtcgcgcaga gagagcacat caatttcaac aattacacct
     9301 attaatacaa caacaataac acaacatgaa ttactgaaag ctgcatgttg taatttaagt
     9361 gagagtttcg aaaccaatcc aactattgat gtaaattaca gcgatgcagt caccggtgcc
     9421 aaacaaattc agatgcttgg acttgatggt atctattctc aaattcagtt tcagaattta
     9481 ccgttgattt ttggcttgtc tgcttctcat ggcttggcat ttacccccgg cccatggatt
     9541 gaatcaatac aaatcaataa aggaataggc tctgtaataa atggttttga agcaatgaca
     9601 ggtcagataa atatagagat gaaaaaacca caaaatgccg atcgcttttt tattaatggc
     9661 tatctgaata atgaaggtcg tgaagaacta aacctgcaac ttgcacaaag gtttaaaaaa
     9721 catatcagca cggtttttct tgctcatggc agcatgaatc atcttaaagt ggacagagat
     9781 aaagatggct ttttagacca acccttaaca aaacaggtta atgcaatgaa tgtttggcat
     9841 ataactgttc ctaataaatt agaaggagaa attggttggc atgctttata tgaaaaccga
     9901 ttgggtggac aaaaaggatt taattatgac gatgatttcg gtaaatcaac tttatacggt
     9961 gtgggtattg aaaataaaat gtttgaagtt tatacaaaaa ccggaactat aaatccagcc
    10021 aaaccatata aaagtattgc attattaacg ctttcacgtt tacacttgca agacaattat
    10081 tatggcaaca aaacatatat aggcgaccag cgtagttttc atgccaatac aatttatcag
    10141 acaatcattc acgacacaag aaattacatt cgttcaggtc tgagttttcg ttataaccat
    10201 tattctgaga aatataattc agtcggaact tataccaatg aaatcattcc gggtgcttat
    10261 gcagagtatt cattcaacaa cgatttaaac tgggcagttg ttgcaggatt aagggccgat
    10321 gttcacaata cttatggttt cttcctgact ccacgtttgc atatcaaata caatattgat
    10381 caactaacag caatacgctt atctgccggc aaaggttggc gcactgccag agtttatgca
    10441 gagaacactt ctatgtttac ttcttcaaga gcattattta ttgatgacct aaaagctgaa
    10501 gaagcatgga atacaggaat caattttaca aagaaaatgc aatggtttgg tcacgatgca
    10561 tcattcaatg ccgactatta ttatacatgg tttaaaaacc aggtggttat tgatatggaa
    10621 gatattcatc aactgcgttt ctataattta gatggaaaat ccttctcaca ttacctgcag
    10681 ggagaattaa atattgaacc tataaataat cttctgttga ggttcggata taaatacaat
    10741 gttgtaaaac aaaccttctc aggtgaatta aaaagcaaac cacttactcc tcgtgacaag
    10801 gcattgttga atgcagaata taaaatcaaa aaggggaaat acagtattga tgcaacatta
    10861 aaatacaccg gcaaaggcag aattcccggt ggaggtgttc aaatgaatgg ttatgaagta
    10921 aaatcaactt ccgaaagttt cattaccttg aattctcagg caacctggag gactaaacat
    10981 gccgattttt acttaggttg cgagaatatt acagacttta gacaaaagca tgcaattatt
    11041 gcagctgatg aaccttttgg tcagaatttt gacgcttcaa tgatttgggg accattaatg
    11101 ggtcggactt tttatgtagg atttcgttat acaattaaat aaacaataaa aatgaaaaag
    11161 ttaattacag gtgccgttat gttatcactc ttcagcttct cattgatggc acaacaaaat
    11221 aaaaaagctg aaataaacat tgctacttcg gcagaatgta ccatgtgcaa aaaagcactt
    11281 gagacagcgc tactacatca gcatggtgtc aaatttgcaa agctcgacct caatacaaga
    11341 gtggttgcca taaaatataa tggtgaaaaa attactcctg aaaccattcg tcaggtaatc
    11401 agcaatgcag gttatgatgc agatagcata aaagccaatc ctgaggcatt tgaaaagcta
    11461 ccggactgtt gcaagaatgg tgcgctgcat cattaggtat tattgaggag aaaatctttc
    11521 agggttcaat tttaaaaccc attcatttaa tgttttaatc gtctgacttt tctctgattt
    11581 ttctgatgtg gacatcaatt attctattaa ctactacaat atcattacct cacacccggc
    11641 tgagtatatc ttctctggaa atatctttgc cgggttttga tgccaataaa gcaactaatt
    11701 taaactcttt acgaggcagc tcaaaagttt gaccttccag atggataaga taatgattac
    11761 ggtcaatcta taatcctcct atcactgcta ttgttgcatc aaccacagac aattcatttg
    11821 cgttgcgcga caatgtttta atggttttat ttagagcagg catagtgtgc actacacgac
    11881 aaacttcaac aacatccata acaggcatca ttatgcccat tacaatctaa tgtggtactt
    11941 cttgtttagc caaaactact gcctcttctc ctttattaac tgtgtaaacc aaaaagccac
    12001 tttcttaaaa attattctaa ccaatccgat aactaattat ttttgccaat tatgggatca
    12061 acagtattac aggaaataaa tggacaggtt gttatattga cattaaatcg tccggaaaaa
    12121 ttcaacagtt ttaaccgtga aatgtctttg caattgcagg atgagctaaa aaaagctgca
    12181 aacaattcag aaataagggc aattttaata tcaggaacag gcaaagcttt ttgtgcagga
    12241 caagatttat cagaagccat tgatgccaac ggcccgggca ttgacgcaat agttcgtgag
    12301 cattacaatc caattattac cttaatcaga aatatagaaa agcctatagt atgtgccgtt
    12361 aatggtgtag ctgccggtgc aggtgctaat attgctttgt gttgtgatat tgttttagct
    12421 gcagaatcgg ccttattcat tcaggcattc agtaaaatcg ggctgatacc tgatagtggt
    12481 ggaacgttct tcttaccgcg tcttattggt tttcaaagag caagcgcatt aactatgttg
    12541 ggtgataaag tttcagcagc agaagccgtt tcaatgggta tgatttataa atcagtagag
    12601 gatacattgc taatggatac tgccatgcag atttcgcaaa cattagccaa tttgccaaca
    12661 aaagctttag gacttacaaa aaaattattg aatcaatcat taagcaatga tttgaataca
    12721 caattggaat gtgaactgga agaacaagtc agtgcagcaa aaagttatga tcatagcgag
    12781 ggtattaaag catttctcga aaaaagaaaa cccgttttta aaggacaatg aaacaacatg
    12841 taggaattgc aggagcaggc tcaatgggat cgggcattgc acaagttgcc gcaacaaatg
    12901 gcaacccagt tacacttttt gatacgaaca gtgaagcatt acaaaaagca caatcatcta
    12961 tccaaagcag cttggacaaa ttggttgaaa aaaataaaat agataagact caatccaatt
    13021 ttattttctc acagataaaa ttcactgaca acataaatga gctaaaaagt tctgaccttt
    13081 tcattgaagc tgttatagaa aatattgaaa tcaaacagag cctgttcaaa aatgttgaag
    13141 gcattgtttc tgacaactgt attttagcta ctaacacttc atcacttcct gtcatcgcta
    13201 taggcgctgc ctgcaataag caagatagga taattggctt acactttttc aaccctcctg
    13261 ttttgatgca tttggttgaa gttattccat catttaacac ctccgaaaaa gtattaactg
    13321 attctgttga aattatccgt caatggggca aaacacctgt tatagccaaa gacactcctg
    13381 gatttattgt taaccggata gcccgccctt attatggtga agcacttaga atttatgagg
    13441 aaggtattgc cgactttgct actattgact atgccatgag aaatatcgga ggatttcgaa
    13501 tgggtccatt cgaattgatg gattttattg gcaacgatgt taattttaaa gtaacagaaa
    13561 cagtatttac acagatgttt tttgacccga gatataaacc ttctattaca caaaaacgat
    13621 tatatgaggg gaagttattc ggccgtaaga gcaacagagg ttattatgac tataaaaatt
    13681 caaatcagcc tcaaccatca ttagacggta acctacataa ccagattttt gatcgcattt
    13741 ttgccatgct ggttaacgaa gctgctgacg cattgatgat gggaattgca actgctgccg
    13801 acattgaaac agcaatgact aaaggtgtca attacccgca agggcttctt gcctgggcaa
    13861 ataaacgtgg cttacggact gtctataata aattggatga cctgtttgcc tactatggag
    13921 aagaccgata tcgagtatgt ccccttttac gaaaattggc tttgaacgat gaaatgttca
    13981 gaatttaagc tgaatttgat aaatatcatc agcattttgc tattacaaac gttatttttg
    14041 cacgcgaatc attttttggg ttagggatgg aaaatcaaga gcagaaaagc aagtttcagc
    14101 aggcaattga cgttattacc ggagttgcaa aaatcaccat tggtgccggc attgtgcatt
    14161 gtaaagcaca agatgaacaa atggatggcc gttatattac tttagagggt caaaagaaat
    14221 tgttttttgg cacttgtggt tatctgggca ttgaaaaaca tccgaaaatt attgaagcag
    14281 cggtagaagc acttcactct catggagcac aattttcgtc ttctcgtgct tatgtatctt
    14341 ctcattatta cgaagaagca gaaagtctgt tttcaaaaat gtttggccgc cctgcattgg
    14401 taatgcaatc gcttacactt ggtcatatca ctggcttacc ccttcttgtt ggcgataatg
    14461 atgcaattat catggatgtt caggcacatg atagtattca atctgccaca gccatgctta
    14521 aattgcgtaa tattaaaata gatatagtcc gccataacag aatggatatt cttgaagata
    14581 gaataaaagt tctaaaaaac agatatcaga aaatatggta tttgggcgat ggtgtttatt
    14641 ctatgcatgg tgattttgca ccggttaagg aactttatgc acttgcagat aagtacgaac
    14701 aattacactt gtatcttgac gacatccatg gtatgagttg gacaggtcca cacggcacag
    14761 gaatggttat gaatgctgtt ccttactatc accgtaaact gtttttatca accggtatga
    14821 caaaggcttt tggtacagcc ggtgggttac ttacataccc tgatgaagaa tattttaagt
    14881 tagttaaaac atgtggtaaa ggatttattt tttcgattca attgccacct gcaatccttg
    14941 ctgcaactat tgcttcgtgt aaaattcaca tgagtgatga gattattcct ttacaacaaa
    15001 gcctgatggc aaaaattgat tatttcaata aaaaagccga agaacttggt ttattggtca
    15061 tcagaaaaga aagatcaccc gtatttttta ttggtgttgg taatcctaat gttggttaca
    15121 gcactgtttt aagaatgaaa aaagcaggtt ttttcttcaa cttatcggta tttcctgcag
    15181 tcagtataaa acacacaggc ttacggatta ctttaagtaa tcacattcag atggaagata
    15241 tagatgaact gttagaaacc cttgcggaaa acttacctgt tgtattcaaa gaaaacaata
    15301 ccagtttcga acagataagg aaagaatttg ccaatcatta atgctcaaag tttttcttta
    15361 gtttcaatat aggcgactca attaatttcc acgataaatg cgaaattaaa aatgtcagtg
    15421 ctattgatac catcattgtc agccacctgt tcggcatttc atatcctgct ctttcaacag
    15481 aactaataaa caatgttgga ataactttgt ggtataaata aattccatag ctgattttac
    15541 cgccataaac cacccacttg ttctccatga atgtttttac aacaccttta aaccctataa
    15601 cagcatttaa tacaagacta cttgatagta aaatcagact taaacataaa aatgggtcat
    15661 tgcgtggctt atcataagat gtaaccaatg ttagcagtat agctaaagga agaagataag
    15721 gtaatgcctt tttgaaatat tctataaacc gcatacgata ctcaaatcca catgccaata
    15781 aagcgcctat gccaagtgta tccatctgcg aaataggcaa taaaaaagga tctcctctta
    15841 acataaaata ctgcctgatg atacatttaa aaagatatcc tgtaataatc atagtgatta
    15901 cccatttaaa ctctttacct tttggcagca gcaataatac aaatggccat aacaaataaa
    15961 attgttcctc tacccctaac gaccaaagat gattatcaat ggttccctgc catccatgaa
    16021 aataaaaaaa ccaattgggt aaataggcca aataaaatac aatccaaccc ttctgcgcaa
    16081 tgtacaatcc aaagcactca agaaaaatat aactgaatat taaaagataa tatggaggaa
    16141 aaagtctgag aaagcgtttg gcaaaaaagt ttttaataat aagtcgtctt gaaattactt
    16201 tctcagcatt tttcagtctc aataaaattc ctgttatcaa aaaaccggat atagcaaaga
    16261 atatctgaac accataaaag cctatagaga acttataatc aaaatttagt tcagctaaat
    16321 aatgatttaa taccaccatc atggctgcaa taaaacgtat tccatccaga tgtggtatat
    16381 accgggtttg gtggtccatt gttgagatta ggccgccaaa atatatcggc taaagttttc
    16441 aacaataaga atttggtaaa tgtcctatat ttaaggttga aggtttatag attttcaaca
    16501 aactttcaat gacatcgaaa caaatgtttc tttaaagaaa actatacctt ttgtaccgga
    16561 aagcaattac gctgccaata tccgtttaga tataaaaagc gatcatcaaa atcgctgatg
    16621 ttcaatggtt taatgaaata tccggctaca ttaaactcat aagctgtctt tacatcagaa
    16681 tcatcttcag aagtagttaa aacacatacg tgaatatctt ttaaatcagg atcagcacgt
    16741 aattcggata aaaactctaa cccattcatc aaaggcatat tgatatctaa taaaataata
    16801 tcaggtcttt ttatcctaat atcactcatt ccacgtaaca tcatcaatgc atcaagacca
    16861 ttatgggcag tgtcaacatg atatggaaaa tcaagtttag cgattgccct cttgattatc
    16921 atgctgtcaa ccttatcatc atcaataaga agtatcgtta attgcttatt cattaatcgc
    16981 caaatatatc acctaattat ttctctgcaa aaataaaaca atttatttgt cagtaacgta
    17041 caaacaccct aagtttaaca caattttcta cacaaattga tatggataca cctgttgtca
    17101 aatggattat cagaaaatta atgctcgata gtatttatcg tagtattcag gatgatgata
    17161 ttctggctaa acaacgtttc aaccttttca gaacctttaa ccttgttgcc atcattggta
    17221 ttggactaat atgttatcag gcaatctatg tttttcaaac ccaaaaaaac ctttcattta
    17281 ttctattagg cttagagttg ttacttattt taaattattt actgcttaat attcataaaa
    17341 aacttaccct atcagttgta attagtttac tgtcttgttt tggagcatta cactacataa
    17401 cctattttgc cggaggcatc cgcaactcag ggatgtttta tcagattgcc tttatcctca
    17461 tttcgtttat gctgctgggc agccgttatg gtatttggtt tacagtattg tctgttgcca
    17521 atatgattta tttctttttg ataactgaat acactaactg ggttgactac agtttcattg
    17581 gcgaaactgc ttctcttatt aatcaggatt atttgattac aagccttgtt agtctgattg
    17641 taatcagcag tctgattaac aatttagaga gtaaaaaaaa tgtagtcata tctaccatca
    17701 cagattccag aaacaaactt actatcatca ataaagagtt gaaaaagctt tccttggttg
    17761 ccagcaaaac tgataatgcc gttgtaatta cgaatgaaaa tggtgtcatt gaatgggtaa
    17821 atgagggttt tacccgaatt acaggttatc aattttatga tgcaaaagat caatctatgc
    17881 cagcattctt aagtggacct gaaaccgata aggaaaatct tcagaaagta caaaacagca
    17941 tgcgcgaatt taaattctgc gacagtgaac ttttgcgtta taagaaagat ggggcaacag
    18001 tgtggttaca tgccagtatt acacctatta gcgacaatga aaaaagttct cgtaaattca
    18061 tctttatcat gagtgatgtt aacgagcgta aaaaggctga aatacagtta aacgaatatt
    18121 taagtgacct tgaaaaaaca aataaagaac ttgacaaatt tgcatacatt gtttcgcatg
    18181 atttgaaagc accattacgt gccattggca acctgacaga ttggattacc caagacagtg
    18241 cagacagcct ttctgatgaa tcgaaagaaa atttccagat tattaaaggc agagtaaaac
    18301 gtatggaaca acttattaat gccattctgg aatattctaa agtatcaaaa cataaaggta
    18361 cacaagagct gttttcgttt aatgaaatta tacacaatgc tgttgattta gcaggcactg
    18421 acagcaactg caaagttgaa atatccggca caatgcctga atactatggc gataaagtta
    18481 aataccagca ggtattcatg aacctaatca gcaatggcat taaacacaac aacaaagaac
    18541 aaaaaaagat tgaaatcagt tgccgcgaag aaggtaagtt ttgggaattt atgattaaag
    18601 acaatggccc cggaattgac aaacgatttc atgaaaaagt gtttgtaata tttcagacac
    18661 taaaagcaag agatgaattt gaatctaccg gaattgggct atcaattgtt aaaaaaattg
    18721 tagaggaggc aggtggtagc attcgcatag aatccactcc cggagaaggt gcagcttttt
    18781 acttcacagt accaaaaaat cagattgatg cttcattaat taccggaaaa aataatcagc
    18841 agatttcacc aatagttttg taagattttt gtttttcttt ttgcaacctg cacacatatt
    18901 catcgtatga aatataaaaa ttcattgtga tgaagcagaa gcaaaaaaaa tcaaacaacc
    18961 tttctaaagt aaaaaatttt gactttgacc aatatctgaa tttaagaaaa gcagagcgta
    19021 ttaaactctt gcagcaaatg gttgaaaaac ttgatccggc ttcaagaaaa aacttcttta
    19081 agagtttgct ccaatatgtt ttgtcagacg aagaacttac tgctttggcg tttaaaaaca
    19141 acagcggtaa gcaagaagta aaaaaatcaa gagttgtaac attgtctgat aaagaaaaag
    19201 aaatgctcgg ccaaattgcc gactactttc atgagttgga gttaataatg gacttggatg
    19261 agttactcat caatccctaa taatcgttac ctttctattt cttaataaaa tccgtcttta
    19321 acttctcaac tattgatgcc acagcaggac aaaccagtgt gttttgataa gttaaatcca
    19381 ttatctgttt gatattttta cggtcagtat gcggatactc tctgcacgct tgcggtcgca
    19441 cagaataaat tgagcaataa ttatcttttc ctaaaaatgg acaaggtgaa taacttagta
    19501 cataatgacc atcagaatca agatatagat atttagctac aaaatctccc ggttttaatt
    19561 tcaaataggt tgcgattcgc tctatatcaa catctctgaa aattggactt gttgttttac
    19621 aacaattggc acagtccaag caactgaatt ccttaaaaaa tccttcactt tcttctctga
    19681 aaaagtgatc taaatgaggc gatttggact tagaaatttt ttctaaaatc tgtttattct
    19741 ctcgcctttt actttgcgct attgataaaa acttcaacat ttcctgtttc atgcacaagc
    19801 aaagaaacaa aattgtgaca tttccgattt cagaagaaac ttcgttaaaa aaaaagtaac
    19861 tttttacttg taggtaggca tcatttttgc tacaattgtt gcgtcatttt atcaatttac
    19921 aattatcatt taccctatat acaacttata actatttgct atgcccaaaa caatttttct
    19981 tattcgccac agcaaagcaa cgcgcgagta ttctcaaatt aatgacagcg acagaccttt
    20041 aacagaaaaa ggttgtcatg atactgaaaa gatgagtttg attttaaaag atcatacaaa
    20101 ctctgttgat ttaattttga caagtacagc tgtcagagct tattcgtctg cactaatcta
    20161 ttgcaaacat cttgactatc atcctaacaa cattaaactt gaaaagaatc tttatatgac
    20221 aggctcaaag gatatgcttg ccatattgaa aaagttagat gagaagatta attcagtaat
    20281 tattattgct cacaaccccg gtatagaaag gttggcaaaa ttacttaccg gcatagagag
    20341 tttacatttt acaaccagtg gcattgcaat gattgattct aagatcaaaa actggagtga
    20401 acttgattcc ggaagttgca acttactctc gtacagacat cctaaagaaa ctatttacaa
    20461 ttagttattg attgacaaaa ccattctttt gcttttcata actttatatg tttttttaca
    20521 aagctatatg taaaataact taggtttggg ttttactatt ttaataaaat tatgaaaaga
    20581 aaaattgagc aagtagcagt tctgggctca ggtattatgg gttctcgcat tgcttgtcac
    20641 tttgcaaatg caggctataa agtaatgtta ctagacattg ctcctaaaga atttacagac
    20701 attgataaaa aagcaggatt tactgaagac agtttgaagt tcagaaataa aatcacagat
    20761 gctcatttac agacagcagt aaaaagtaac ccatccccac tctatcataa aagttttgcg
    20821 caaagaattt caacaggaaa ttttacagac aatctaaaag acattgctaa ctgcgattgg
    20881 attatagaag ttattattga agatttaaat attaaacgca ctttattcga gcaagtagaa
    20941 aaattcagaa agcccggtac attaattaca tcaaacactt ccggaatacc cattcattta
    21001 atgacagaag gtcgctctga cgattttaaa aaacatttct gcggaacaca cttttttaac
    21061 cctccacgtt accttcctct acttgaaatt attccaacgc aatttaccga taaggatgtg
    21121 attgatttta tctcacactt cggtgatcgc tacttaggta aaacaacggt tttatgcaaa
    21181 gacactccag catttattgc aaacagaatt ggtgtatttg gtatcatgtc attgtttcac
    21241 tcaatggaaa aaacagggct ttcgctttct gccattgata aacttaccgg tccaattatt
    21301 ggcagaccaa agtctgcaac tttcagaaca agtgatgttg ttggattaga tactttagtt
    21361 catgtagcaa atgatgcata taaaaatttg cccaacgatg aacaaagaaa cctatttgct
    21421 ataccaccat ttatcaataa aatgattgag aacaaatggc tgggagataa aaccaaacaa
    21481 ggattttaca aaaaaacaaa atctgctgag ggtaaaacag aaattcttga actggactta
    21541 aaaagtttag aatacaagcc atcaactaaa gtaaaattta aaacaattga aagcacaaaa
    21601 actgttgatg acctgaaaca aagattgcct atgttacttg gtggaacaga tgaggcagcg
    21661 gcattttatc gttccatgtt tttttcatta ttcacttacg
//
LOCUS       DAOQNP010000055        21683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_824_length_21683_cov_9.229980, whole genome shotgun sequence.
ACCESSION   DAOQNP010000055 DAOQNP010000000
VERSION     DAOQNP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21683
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_824_length_21683_cov_9.229980"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PKY09_08480"
     CDS             <1..1268
                     /locus_tag="PKY09_08480"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB12932.1"
                     /translation="NGIIERIDPSINVKHKVEEIAGDGLILMPGAIDDQVHFREPGLT
                     HKGEIASESAAAVAGGITSFMEMPNTVPNTLTQELLEEKYQLAQKKSWANYSFYMGAG
                     NNNLDEVLKTNRENVCGIKIFMGSSTGNMLVDNLVTLEDIFSQSPMLIATHCEDEQTI
                     RQNTEKYKSLYGDKLNVSYHPLIRSEEACYLSSSLAVNLAKKYNTRLHILHISTARET
                     ELFKNDTPLRDKRITAEACIHHLWFDDNDYAVKGNFIKWNPAIKTKQDKESILAAVLN
                     NHIDVIATDHAPHTLEEKQQPYLTAPSGGPLVQHAVVAMLELYHQKKISLEKIVKKMC
                     HAPAICFRIKNRGFIREGFAADITLVALNEPWTVSKSNILYKCGWSPFEGTTFQSKVK
                     HTFVNGAHIFNNGKLQLHTNGQRILFSSH"
     gene            1323..1637
                     /locus_tag="PKY09_08485"
     CDS             1323..1637
                     /locus_tag="PKY09_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNB12933.1"
                     /translation="MSCTSKSNEVVIPEAILSKDSMIQILADIHLAESEATLYPYNDS
                     LGIINLPAYYKYIFEHHKIDTAVFSHSMNFYLANPELMNSVYSGVIDELSKRHARYQK
                     NN"
     gene            complement(1634..2821)
                     /locus_tag="PKY09_08490"
     CDS             complement(1634..2821)
                     /locus_tag="PKY09_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNB12934.1"
                     /translation="MKQQNKYSQRGVSADKEDVHAAIKNVDKGIFPKAFCKIIPDYIS
                     NDKDYCLLMHADGAGTKSSLAYAYWKETGDISVWKGIAQDALIMNIDDLLCAGATDNI
                     LLSSTIGRNKNLISGDVIAAIINGTEKLVDELKNYGVNITTTGGETADVGDLVRTIIV
                     DSTVMCRMKRSEVIDNANIVAGDVIVGFASFGKATYETEYNSGMGSNGLTSARHDMFN
                     KSVAAKYPETFDNTLPEDIVYNGNFRLTDSITLDDGYGQVSMHAAKAVLSPTRTYAPL
                     VKKIFETHRKNIHGMIHCSGGGQTKVLHFVDNLHVIKDDLFNTPTLFKLIQESAGTNW
                     HEMYKVFNMGHRLEVYCPKAIADDLISIAAGFEIGAQIVGRVENCDGKKLTINSPYGK
                     FEY"
     gene            complement(2905..>4542)
                     /locus_tag="PKY09_08495"
     CDS             complement(2905..>4542)
                     /locus_tag="PKY09_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB12935.1"
                     /translation="FNAFKALEPGDNRGQDGATSSETSQQWKDKPTKYKVENVAALNT
                     KYFDFAVAVSNLEKNTLYFTSSRAEASGNKNDAWYGEKFYDIFKSAQDNNGKWSIPTP
                     ISEAINTAASEGAMTFDAKGNVMYFTRCQKSDEKGKEGQCKIYKSVYDGAKWGTPELL
                     PFNSDNYTCGHPFLTEDGTTLYFSSDMPGGAGGKDIWMTKNENGAWGKPVNISSINTA
                     GDEMFPYIAPNGKLYFSSNGHMGMGGLDIFTATLENNAWTNVTNLKYPINSSWDDFGM
                     MFNTATTGYLTSNRDGGQGQDDIYSFVVPPANFAVYGRVYDTDTKEPISGATVELFGS
                     DGTQLSVKTQDAGTYRYELKPDVDYKVSASFTGYLTQFKELTTKGLDESKDFEQNFDF
                     PLKSTSKPIALPEIFYDLDKYSLRPESKAALDGLIKVLDDNPTITIKLTAHTDFRADD
                     NYNLALSKKRALAAYNYLVSKKVDKDRLSWEGRGEKDPKEVENDEQYLPFKRGDKLTE
                     EFIKGLTSEEMKEKAHQYNRRTEFTVLSTDYTPKKAK"
     assembly_gap    4543..4642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4894..5649)
                     /locus_tag="PKY09_08500"
     CDS             complement(4894..5649)
                     /locus_tag="PKY09_08500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNB12936.1"
                     /translation="MIKKLLAVLMFISGVAQAQIFRLTYNTTVCPSPLNATTGVYLYA
                     GANITSPGAAPGYFMGSINPTAYPLYQTQTGVWEICFNPFQTFRDGAGSLIPAGSTIY
                     SIDVNFMDAAGSIFTGNCQHNYIRITTPMSSSSSNFPAIASGLVVATCNVGLQEISNA
                     QAVISQSANPLTSNTTFRLLLKDPAKVNLDIYNVLGKKVCSIVDGKKLSGYTEFNWDG
                     TAENGSLLANGCYYYSLKLNDKIYTSNKLIIAR"
     gene            complement(5784..5975)
                     /locus_tag="PKY09_08505"
     CDS             complement(5784..5975)
                     /locus_tag="PKY09_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12937.1"
                     /translation="MTIKKGDKVSFNLPGTGNRQVAEVTDVLEKCVQVKYNNESYSNL
                     LTLDKDKILETLPEAPKME"
     gene            6196..9162
                     /locus_tag="PKY09_08510"
     CDS             6196..9162
                     /locus_tag="PKY09_08510"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB12938.1"
                     /translation="MNKQFYLLILFLTLTISFSARSQSSLSEKSLSIENDFSVEVFNT
                     ESGFPQNSVIDILQSSNGYLWFSTFSGIVRYDGLKFTVFSNNETKGLLSNTVLHIKED
                     KYKNLWFLDDAGHLVKYDGNIFTGFEKSFGNIKISDFCFSEDGTLYVSTTNNEIYTVN
                     GNILDFGTRINQYKILLITAGKNNSLLIATTGGLFELKNGNIQQIESIPKTKVLSLCY
                     DRDNFLWVGTTAGIYKVSAAKTEIVNLPDESEGYSKIFVDRQNRKWIFDGYDGIYLLG
                     TNDFNQISEESGLSSNNIKVVYQDSEDNIWVGTSNAGVNKLTYKIFRTFSVEDGLIAD
                     GVAPIVKSANGAVYVGNNCGGINKIIDGKIIKIPEPKENTCIWSMLEDKDENLWVGTY
                     GGGLFKFKNDKEVFHFNKSNGLADDVVFALYQDSKGIIWIGTDKSIFTIQHDTISHFA
                     KGIIKTKVTYFTEDSVGNIWAASNNGLYRITNAQKIDLFTTYHGLPSNSIRSLYFDEE
                     KTLWIGTVRGGFSRLKAGKFVSFTNIPELSTLDVFCIVEDNEQNLWFTTNKGIYNVKK
                     YDLNNYADQKSATLSFNYYDRKDGLKTSEFNSGFQPNYLKEDPTHFWFPTIKGVAILN
                     TRRIIKSDYIPQIIFENIIADGKEILPLGNINLHQKVKTLEINYTAPKFNNPQKIFFQ
                     YKLDGVDGDWQTATTERTVRYFNLNPGNYTFKIRLYGIFGTEKSLSFYVPMPFYKSNI
                     FLVILYVTGIAFLGLIAYFRIKHIRNQEKKKTALNKRFAEFELKALQAQMNPHFLFNC
                     LNTIKFFITTNNHSAANKYLTKFSKLLRMFLDHSTSESITIEEEINMLRLYIELEQMR
                     FDEGFNFHLQVDESIDYKNTEIPATILQPFVENAINHGLVNLNRQGNLTLIFEQKNGC
                     IVGIIDDDGIGRAEAARLKNHSTSQHISRGTQLIDDRIKTLNSIREQHIEIQIIDKED
                     DQHNATGTRVIVTIPI"
     gene            9174..9920
                     /locus_tag="PKY09_08515"
     CDS             9174..9920
                     /locus_tag="PKY09_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB12939.1"
                     /translation="MLQTLIVDDEKRGRELLKMILATNCPEVKISGEASNIKEAYQMI
                     LQNEPDLVLLDIEMPGGTGFDLLSKFDEVNFDVIFITAFDQYAIKAIKFSAMDYILKP
                     VDEEELIKAIKRAEQNRNRKNNKEKVNSLISNVQKSSKHQKIGLTSGEGLEFIEIKNI
                     LRCEADGKYTSVFLTDGKKLLVSKNLKEFEDLLTEYNFFRTHHSHLVNLDCIKKYQSG
                     RGGYVVMSDGSTITVSQRKKDDFLSSLKKI"
     gene            9999..10460
                     /locus_tag="PKY09_08520"
     CDS             9999..10460
                     /locus_tag="PKY09_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNB12940.1"
                     /translation="MKHLFLVLCIAATAFISCKSDQQKKTIEPEMVSNPASAEPANAD
                     TSLLPKFDFESDNHEFGPITEGEIVSYNFKFKNSGKAPLIITQASASCGCTVPEYSKD
                     PIAPGQEGFIKVTFNSEGKHGMTSKTITLLANTIPNTKVLTISADISKKDK"
     gene            10460..10780
                     /gene="yajC"
                     /locus_tag="PKY09_08525"
     CDS             10460..10780
                     /gene="yajC"
                     /locus_tag="PKY09_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNB12941.1"
                     /translation="MYLTILLQSQGNMAMQQFLMLGLIIVIMYFFMLRPQMKKMKVEK
                     QFKESINKGDKVVTIGGVHGKITEVGETFFMIEIDNNVRIKIDKTAVSAEASKQYRKV
                     EDKK"
     gene            10793..11740
                     /locus_tag="PKY09_08530"
     CDS             10793..11740
                     /locus_tag="PKY09_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNB12942.1"
                     /translation="MNIFRNIRFKGNASLPVFFICVFVSLVFWILYSLNKNYVYPVTY
                     TVTLQNTPFSKQVISEIPQQVNVTLRSRGFELFWYLIRNRERNLIVDLKKYTVNNEKL
                     GISLQSEFSNQNHSRLKEMDVVTTLPDSVWLYLSSKYMKTVPVVTQFTYSIKKNFGLS
                     GKVSIVPDSITISGEKSQLDGVNEIKTVAASYDNLDRDLKATLSLTLPAGKVNLSQKE
                     IKIHIPVEEFIEKKIPIALNYSINGSGKVILIPDKAELIIQVPLKRYEQLNADSFQVM
                     VSAKRYLKEGQLAVELISKPQGINVSSIIPSTVNYYIER"
     gene            11740..12321
                     /gene="coaE"
                     /locus_tag="PKY09_08535"
     CDS             11740..12321
                     /gene="coaE"
                     /locus_tag="PKY09_08535"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016775932.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNB12943.1"
                     /translation="MKKIGITGGIGSGKTFVANIFKTLGIPVYDADNAAKQLMNHEPL
                     RNKLIQLFGNSIYNEQGELNRPVLASLIFNDKKKLDAVNALVHPAVAQHFNIWCNKQQ
                     ADYILKEAAIMFESGANKILDAVICVTAPETTRIERAMKRDNTSEEKIKAIIARQLNE
                     EDRNKRCNYLIKNDGTEPLLPQVLKIHDLLKGK"
     gene            12490..12792
                     /locus_tag="PKY09_08540"
     CDS             12490..12792
                     /locus_tag="PKY09_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNB12944.1"
                     /translation="MKEYQASRMSEGNKVLPNKIRIDNDGITFRIPGVFSGEEKTIPF
                     SRISSVEVNTPLVGFSSITINTTGEGAIVATGFYKEDVLEIKKIVLEKINIIQNKS"
     gene            complement(12961..14610)
                     /locus_tag="PKY09_08545"
     CDS             complement(12961..14610)
                     /locus_tag="PKY09_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB12945.1"
                     /translation="MQSDQKKNFQPLWYALLITVGIVIGVSLNSSFKGGTNILFSRSA
                     SGGFNKINDVVNYIRQEYVDTINQQELVNSSITQILQNLDPHSSYIPADELKSVNEPL
                     EGNFEGIGIEFHIQYDTIMVVSTIAGGPSETIGLRPGDRIVEVDKKKVAGIGIKNEDV
                     FKYLRGPEGTKVALKVKRSGIEKLLDFTIVRGKIPIRSIETSFMANANTGFIKISRFS
                     ATTYDEFMEAVEALKKQGMQNLILDLRGNPGGYLDAATMLADEFLPDKKLIVYTEGKA
                     RPRKNYYATEKGDFEKGKVFVLIDEGSASASEILAGALQDWDRAEIIGRRSFGKGLVQ
                     EQTLFPDGSAMRLTVARYYTPTGRSIQKPYKNGYMAYEEEVYDRYKHGEMVNADSTHF
                     NDSLKYTTPGGKIVYGGGGIMPDLFIPIDTTSDNEFARSVFGLGLVSRFSYDYTDKHR
                     NKLNTFKSIDDYIKGFSADQRLLSDFFVYAIDNKVRPDEKSIQASKQLIANQLKANIG
                     RLLFGNDAFYRVVLSDDKAFNAALQQAESTVQSKVKESEMR"
     gene            complement(14620..15093)
                     /locus_tag="PKY09_08550"
     CDS             complement(14620..15093)
                     /locus_tag="PKY09_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNB12946.1"
                     /translation="MGSPGFDDIYMDLAKTLALKSHCVKMQVGAVLTKDTRIISLGYN
                     GPPAGTHNCDTEWPDDGCPRDSKGGCSLAIHAEQNAVLYAAKNKVSVEGATLYVTLSP
                     CLACAKVVFTAGIKKVIFLNSYAQYKGIKSDEGVDFLRKFNVEVQQYTPGTVSGT"
     gene            complement(15139..15222)
                     /locus_tag="PKY09_08555"
     tRNA            complement(15139..15222)
                     /locus_tag="PKY09_08555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15186..15188),aa:Leu,seq:caa)
     gene            15553..19383
                     /gene="cas12a"
                     /locus_tag="PKY09_08560"
     CDS             15553..19383
                     /gene="cas12a"
                     /locus_tag="PKY09_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HNB12947.1"
                     /translation="MQQFINKYTLSKTLRFELKPIGKTLENIQTKGLLQQDAIRAKSY
                     QEVKKLIDRYHQYFIELALDGLKLNNLQDFQEQYLASPEEKKKEDFKKKFEKNQDDLR
                     KEIVAAFKTGDANEIFKVLDKKELITELMVAWLTKQEDKDRIEEFKTFTTYFIGFHEN
                     RKNMYTDKAQSTAIAYRLVHENLPKFLDNIKVFEKLKAVPELHEKCSVLYKEIEEYLN
                     IRSIDEAFELDYYNSVLTQKQIEVYNLIIGGRVAKENEKKIQGLNEYINLYNQPKDKK
                     NKIPKLKPLYKMILSDRESVSFLAEKFENSQEVLKAIQEYYHANIKSYQSSEKDGAEN
                     VLERLQELLANLSSNNLSQIYIRNGKTITDISQALFGDFSLIKDALKYSFTNTLEIGR
                     NGLSKKQEEVIEKYLKQDYFSITEIEVALWNYRTENDLLKDLEEEQHLIANYFNSYFK
                     TTVNDKEYDLVANVTAKYSCIKGLLEQDYPEDKKLNQETKEIDDIKAFLDALMLVLHF
                     VKPLMLPHDSTLEKDQSFYNTIAPYYEELQYLISLYNKVRNFASQKAYSVEKYKLNFE
                     NSTLLAGWDVNKEPDNTSVLFRKENDYFLGVMDKKHNGIFKKTPKSKTQDTYSKVNYK
                     LLPGASKMLPKVFFSRSNIAFYNPSEEIITIRNHSTHTKGGTPQTGFEKKDFNLSDCR
                     KMIDFFKDSIAKHPDWKQFGFNFSDTDSYDSIDGFYREVEAQGYTISYTEIDETYIHQ
                     LVDEGKLYLFQIYNKDFSEFSKGKPNMHTLYWKALFDSENLRDVVYKLNGQAEIFYRK
                     SSIKKDKIIVHKANEKVANKNPLNTKKESLFTYDLVKDKRFTVDKFQFHVPITLNFKA
                     SGNDYINQEVLSFLRQNPDVNIIGLDRGERHLIYLSLINQKGEILQQFSLNDIINEYK
                     GITHKTAYKTLLEKKEKERADARENWGTIESIKELKEGYISQVVHKIAKMMVEYNAIV
                     VMEDLNMGFKRGRFAVEKQVYQKLEKMLIDKLNYLVFKDKAPNEAGGLYNALQLANKF
                     ESFQKMGKQSGFLFYVPAWNTSKIDPTTGFVNLFYTKYESIEKAQKFFEKFDSIHFNT
                     KEQYFEFAFDYDNFTERATGTQTQWTVCTQGERILTFRNPSANNQWDNKEIKLTEQFE
                     DLLGKHNISYGSGTCIKAQVATQSGKDFLKGMMDLFKLTLQMRNSITNSEVDYLISPV
                     RNAQGSFYDSRNADATLPKDADANGAYHIAKKGLMWLHQIHEFEGNDWRKLDLDKTNK
                     GWLNFVQPKNKTNE"
     gene            19376..20416
                     /locus_tag="PKY09_08565"
     CDS             19376..20416
                     /locus_tag="PKY09_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNB12948.1"
                     /translation="MSKKKDKHTPNIRSSAAEYLTFIAATGDNEQSVEMRYEDENIWL
                     TQKMLAILYDVEVNTINYHIKKIFEDAELEENSVIRKFRITATDGKNYETNHYGLQMV
                     MSVGFKVNSERATQFRKWVGQIAKDYTIQGWVMDVPRLKQSGTILTKEYFEKQLEKIR
                     EIRLSERRFYQKITDLYATSLDYDKSASTTKRFYATVQNKLHWAIHRHTAAELIVKRA
                     NHQKENMGLSTWEAAPEGKIQKSDVSIAKNYLTQNEMASLERIVSAYLDLAEDFTLRG
                     IPMTMQDWEIRLNKFLEFNERDILDNPGKVTHEIAQAFAESEFEKFRIVQDKLYQSDF
                     DKLLEHTKNRKP"
     repeat_region   20883..21177
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20883..20918
                     /rpt_unit_seq="ggcaaataagccaatcaaatttctactattgtagat"
     gene            21290..>21683
                     /gene="prs"
                     /locus_tag="PKY09_08570"
     CDS             21290..>21683
                     /gene="prs"
                     /locus_tag="PKY09_08570"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNB12949.1"
                     /translation="MAREVKLFSGEATRYLADNIALSYGLPLGDVIVSHFSDGEFSPY
                     FDETVRGCDVFIIQSTFAPTNNLFELLLLIDAAKRASAHYITAVIPYFGFARSDRKDK
                     PRVAIASKLVANLLTAAGVTRVMTMDLHA"
BASE COUNT         7285 a         3931 c         3873 g         6494 t
ORIGIN      
        1 gcaatggcat cattgaaaga attgaccctt caataaatgt aaaacacaag gtagaagaaa
       61 ttgccggtga tggtttaata ctaatgccgg gtgctattga cgatcaggtt cactttcgtg
      121 agccgggtct tacacataaa ggtgaaatag cttccgaatc ggcagctgct gtggcaggtg
      181 gcataacttc attcatggaa atgcctaata ccgtaccgaa cacattgaca caagaactat
      241 tagaagaaaa atatcagctt gctcaaaaaa aatcatgggc taactattct ttctacatgg
      301 gtgcggggaa caacaacctt gacgaagtgc ttaaaaccaa cagagaaaat gtttgtggca
      361 taaaaatttt tatgggctcc tcaacaggta atatgctggt ggataatctt gttacattag
      421 aagatatttt ctcgcaatcg ccaatgctca tagccacaca ttgcgaagac gaacaaacca
      481 tcagacaaaa tacagagaaa tataaatctc tttacggaga taaacttaat gtatcatatc
      541 atcctctaat ccgcagtgaa gaagcttgct acctgtcaag ttcattggca gtgaatcttg
      601 caaaaaagta taatacccgt ttgcatattc ttcatatttc aacagctaga gagacagaat
      661 tattcaaaaa cgatactcct cttagagata aaagaataac tgctgaggca tgtattcatc
      721 atctttggtt tgatgataac gattatgctg taaaaggtaa tttcatcaaa tggaatcctg
      781 caattaagac aaagcaggac aaagagtcta ttttggcagc agtattaaac aaccatattg
      841 atgtcatagc taccgatcat gcaccacata cactcgaaga aaaacaacaa ccttacctta
      901 ctgcgccttc aggcggcccc ttagtgcaac atgccgtagt ggctatgctt gaactttatc
      961 atcagaaaaa aatttcactc gaaaaaatag tgaagaaaat gtgccatgca ccggctatat
     1021 gtttccgtat aaaaaaccgg ggatttattc gcgaaggttt tgctgctgat attacattag
     1081 tagctctgaa tgaaccatgg actgtttcta aaagcaatat cctttataaa tgtggctggt
     1141 cgccttttga aggtacaaca tttcaatcaa aagtaaaaca tacttttgta aacggtgcgc
     1201 acatttttaa taatggcaag ctgcaattac acaccaacgg acagcgaatt ttgttttctt
     1261 ctcattaaaa tatgcaagtg aaaaaatacc tcttactttt ttttattcta atattatttt
     1321 ttgtgtcatg cacatctaaa agtaatgaag tagtaattcc ggaagctata ttgtcaaaag
     1381 acagcatgat acagatactt gctgatatac atcttgctga atcagaagca actttatatc
     1441 cttacaatga ttcattaggc atcattaacc ttcctgctta ttacaaatat atctttgagc
     1501 atcataaaat tgatactgcc gtattttcac acagcatgaa tttttatctt gctaatcctg
     1561 aattgatgaa ttcagtttac agtggcgtta ttgatgaact gagtaaacgc catgcccgtt
     1621 atcagaaaaa taattaatat tcaaattttc cgtagggcga atttatggta agttttttac
     1681 catcacaatt ttcaacacgc cctacaatct gcgcaccaat ttcaaatccg gcagcgattg
     1741 aaatcaaatc atcggcaata gctttagggc aataaacctc cagacggtga cccatgttaa
     1801 atactttata catttcatgc cagttggttc cggcagattc ctgtatcagt ttgaaaagtg
     1861 ttggcgtatt aaatagatcg tcttttatga catgaagatt atcaacaaaa tgaagcacct
     1921 tggtttgtcc tcctccactg caatgaatca ttccgtgaat attttttcta tgtgtctcaa
     1981 atattttttt tactaatggt gcataggtac gtgtaggcga tagcactgct tttgcagcgt
     2041 gcatggaaac ttgtccgtag ccatcatcaa gagttattga atcggtaagt ctaaagtttc
     2101 cattataaac tatatcttca ggcaaggtgt tgtcaaatgt ttcgggatat tttgccgcca
     2161 cacttttatt aaacatatca tgtcttgccg aagtgagtcc gttacttccc atgccgctat
     2221 tatactccgt ttcataggtg gccttgccga aagaagcaaa gccaacaata acatcaccgg
     2281 caacaatatt tgcattatct attacttcac ttcttttcat tctgcacata accgtgctgt
     2341 caacaattat ggtgcgtact aagtcgccaa catcagcagt ctctcctccg gtagtggtaa
     2401 tgtttactcc gtaatttttc aattcatcaa ccaacttttc agtgccatta ataatagctg
     2461 ctatgacatc accggaaatc agatttttat ttctgccaat tgttgaactt aataaaatat
     2521 tgtcagtagc acctgcacaa aggaggtcgt caatattcat aatcagggca tcttgagcaa
     2581 ttcctttcca aacagaaata tctcctgttt ctttccaata agcataagcc aatgatgatt
     2641 tagttccggc accatcggca tgcatcaaca gacaataatc tttatcgttg cttatgtagt
     2701 ccggaatgat tttacagaat gcttttggaa aaattccctt gtcaacattt ttaatagctg
     2761 cgtgtacatc ttctttgtct gctgaaacac cacgctggct gtacttgttt tgctgtttca
     2821 tcttcctgta tccataaacc tttgcaaaac taaaaaagca acccaagacc gggttgcttt
     2881 tttagtgtaa ttcttataca tcaattattt tgcctttttg ggtgtatagt ctgtgcttag
     2941 tactgtaaat tctgttctac ggttgtactg atgcgctttt tccttcattt cttcagaagt
     3001 caacccttta ataaattctt ctgtaagctt atcaccacgt ttgaacggca gatactgctc
     3061 atcgttttca acttctttag gatctttttc tcctcttcct tcccagctca aacgatcttt
     3121 gtcaactttt ttagaaacca aataattgta agctgctaaa gcacgttttt tagacagtgc
     3181 caagttgtag ttgtcatcag cacggaagtc agtgtgtgca gtaagtttaa tggtaatagt
     3241 tgggttgtca tccaacactt taattaagcc atcaagtgct gcttttgatt caggacgtaa
     3301 tgaatacttg tcgaggtcat agaaaatttc cggtaatgca attggctttg aagttgactt
     3361 caatgggaag tcgaaattct gttcaaaatc ttttgactca tcaagacctt ttgttgtcag
     3421 ttctttaaat tgtgtcagat agcctgtaaa tgatgcagaa actttatagt caacatcagg
     3481 ttttaactca taacggtagg ttcctgcatc ctgtgtttta acagaaagct gtgtaccatc
     3541 gcttccaaaa agttcaaccg ttgctcctga aattggctct ttggtatcgg tatcatacac
     3601 tcgaccataa actgcaaagt tggcaggagg aactacaaaa gaataaatat catcctgacc
     3661 ttgtccgcca tcacggtttg aagttaaata accggttgta gcagtattaa acatcattcc
     3721 aaaatcatcc catgaagagt tgattgggta ttttaagttg gtaacattag tccaggcatt
     3781 attttctaag gttgcggtaa agatgtcaag acctcccata cccatgtggc catttgaaga
     3841 gaaatataat tttccatttg gcgcaatata tgggaacatt tcatcacctg cagtgttgat
     3901 tgaagaaatg tttacaggct ttccccatgc accattttca tttttagtca tccaaatatc
     3961 ttttccaccg gcaccacctg gcatgtcaga cgaaaagtaa agtgtagttc cgtcttctgt
     4021 caagaaagga tgtccgcagg tataattgtc gctgttaaaa ggtaataatt ccggagttcc
     4081 ccactttgca ccatcataaa cactcttgta aattttacat tggccttctt tacctttttc
     4141 atcactcttc tggcaacggg taaaatacat tacattgcct tttgcatcaa atgtcatagc
     4201 accttcagat gcagcagtat taattgcttc actgattggt gttggaatgc tccattttcc
     4261 attgttatct tgagcagatt tgaagatgtc ataaaatttt tctccatacc atgcatcgtt
     4321 tttatttcct gatgcttctg ctcttgaaga ggtaaaataa agtgtgtttt tttctaagtt
     4381 ggatacagca acagcaaagt caaaatactt ggtatttaat gctgcaacgt tttcaacctt
     4441 atatttagtt ggcttatctt tccactgctg tgaagtttct gatgatgttg caccatcctg
     4501 accacggttg tcaccgggct caagtgcttt aaatgcatta aannnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nngcagtttt gcttttcttg gcttttgccg catccatagt
     4681 ggttactgaa aagatgcagg caatagataa taatagtaat gataacttct tcataataat
     4741 ttttatggtg ataatattca gttaggctta tcagaggcaa agaaaaaaat aatttccttc
     4801 atatcaaaaa caatcgtcag aaaagataaa acaaagtgtt tggggttaat taaaatccac
     4861 aaaaacatgg gttccttctt gtgttttccc tccttatcgg gctataatca gtttgttact
     4921 tgtataaatt ttatcgttca gtttcagtga gtaataatag cagccgtttg caagcaaact
     4981 accgttttct gctgttccat cccagttaaa ttccgtgtaa ccacttagtt ttttgccatc
     5041 cacgatgcta caaacctttt tgccaagaac attataaatg tccagattta ctttagccgg
     5101 atctttaagc aacaaacgga atgttgtgtt tgatgttaat ggatttgctg attgtgagat
     5161 aacagcctgt gcatttgaaa tttcctgtaa gccaacatta caagtagcta ccaccaagcc
     5221 tgaagcaatt gccggaaagt tagaagatga actactcata ggtgtggtta ttctgatata
     5281 attatgctga caattacctg taaaaataga gccggctgca tccataaaat tcacgtcaat
     5341 actatatata gttgatccgg ccggaattaa acttcctgca ccatcacgaa atgtctgaaa
     5401 aggattgaag caaatttccc atactccggt ttgtgtctga tataaagggt aagcagttgg
     5461 gttaatacta cccataaaat aacccggtgc tgcaccagga gaggtaatgt ttgctccggc
     5521 ataaagataa acacctgttg ttgcattaag tgggcttggg caaacagttg tgttgtaggt
     5581 caatctgaaa atttgtgcct gagcaacacc tgaaatgaac attaaaactg ctagtaattt
     5641 tttaatcata aagaatttga aatttttgca aagataatca gataattcaa tgtttttaaa
     5701 cgttttaaac attgattaaa aaagtgcaaa aggccgggat ttctcccagc ccttaaccta
     5761 aaaacagaaa ataaaccgca tctttactcc atcttgggag cctctggcaa tgtttccaga
     5821 attttatctt tatctaatgt cagcaaattg ctgtatgatt cgttattata ttttacctga
     5881 acacattttt caagcacatc agtgacttcc gctacctgac gattccctgt tccgggaagg
     5941 ttaaatgaaa ctttatcgcc ttttttgata gtcataatct attgctgaaa tcttttacga
     6001 atataatgtg atttggcaat aagcaaaagc cggaaagtaa caattgagca tttttaagat
     6061 aagattaagt caaataacaa gataatttta aataaaagta cagcaacacc tttttttatg
     6121 taaccctatt tgcagcacct gttactttaa acaaattaat agatgatttt tatttaattt
     6181 ggcagcacaa tcaacatgaa taaacaattt tatttactaa ttttattttt aaccctgaca
     6241 atatcttttt ctgcacggtc acaatcatca ttgtctgaga aaagccttag tattgaaaac
     6301 gatttttctg ttgaagtatt taatactgaa agtggtttcc cgcaaaattc tgtaatagat
     6361 attctacaat catcaaatgg atatttgtgg ttttctacat tcagtgggat agtgcggtac
     6421 gatggcttaa agtttacagt tttcagtaat aatgagacta aaggtttact gtctaacaca
     6481 gtcctacata ttaaagaaga taaatataaa aatctttggt tcttagatga tgccggtcat
     6541 cttgtaaaat atgatggtaa tatttttacc ggatttgaaa aatcatttgg caatataaaa
     6601 atttctgatt tctgtttttc cgaagatggt acactttatg tttccacaac caacaatgaa
     6661 atttacaccg ttaacggcaa cattctcgat tttggcactc gtatcaatca atataaaatt
     6721 ctcttaataa ctgcaggtaa aaacaacagt ttattaattg ccacaacagg tggtttattt
     6781 gagttgaaaa acggtaacat tcaacagata gaaagcattc caaaaactaa ggttttaagt
     6841 ctctgttacg accgcgacaa ttttctttgg gttggaacaa cagccggaat ttataaagta
     6901 agtgcggcaa aaaccgaaat agttaatctt cccgatgaat ccgagggtta ttcaaagatt
     6961 tttgttgacc gacaaaacag aaagtggatt tttgatggct atgatggcat atatctttta
     7021 ggaacaaatg attttaatca gatttctgaa gaatcaggat tgtcatcaaa caatatcaag
     7081 gtagtttatc aggattcgga agacaatatt tgggtaggaa ccagcaatgc gggagtaaac
     7141 aaacttactt ataaaatatt cagaacattt tctgttgaag acggccttat tgctgatggt
     7201 gttgcaccta ttgttaaatc agccaacggt gcagtttatg ttggcaacaa ttgtggcggc
     7261 atcaataaaa ttattgatgg aaagattatt aaaattcctg aaccgaaaga aaacacatgc
     7321 atttggtcaa tgcttgaaga taaagacgaa aatctttggg ttggcaccta tggtggcgga
     7381 ctattcaagt ttaaaaacga taaagaagtt tttcatttca acaaatcaaa tggccttgct
     7441 gatgatgttg tttttgcctt atatcaagac agcaaaggaa taatttggat tggaaccgat
     7501 aaaagtattt tcactattca gcatgatact ataagtcact ttgcaaaagg tattattaaa
     7561 accaaggtga cttattttac cgaagattcc gttggaaata tttgggctgc aagtaataat
     7621 ggtttgtata gaataacaaa tgcacaaaag attgatttat ttacaaccta tcatgggttg
     7681 ccatccaact ctataagatc gctttatttc gatgaagaaa agactttatg gataggtaca
     7741 gtaagaggtg gcttttcaag attgaaagcc gggaaatttg tgagtttcac caatatacct
     7801 gagctttcaa cattagatgt tttttgcatt gtggaagaca atgagcaaaa tctgtggttt
     7861 acaacaaata aaggtattta taatgtaaaa aaatacgacc tcaacaatta tgctgaccag
     7921 aaatcagcaa cactttcctt taattattac gacagaaaag acggattaaa aacctcagaa
     7981 tttaattctg gatttcagcc taattatctg aaagaagacc ctacacactt ttggtttccg
     8041 acaataaaag gtgttgcaat tctcaatacc agaagaatta tcaagagcga ctacatcccg
     8101 caaataatat ttgaaaacat tatagctgat ggcaaagaaa ttttaccatt aggtaatatt
     8161 aatctgcacc aaaaagtaaa aactctggaa ataaattata ccgctcctaa attcaataat
     8221 cctcagaaaa tattttttca atataagtta gatggtgttg atggcgattg gcaaacagca
     8281 actacagaac gcacagtgcg ttattttaat ctgaacccgg gcaattacac ttttaaaatt
     8341 agattgtacg gcatttttgg tacagaaaaa tccttaagtt tttatgtgcc aatgcccttt
     8401 tacaaaagca atattttctt agtaatttta tatgtgacag gtattgcatt tcttggtttg
     8461 attgcatact tcagaattaa gcatatacga aaccaggaaa aaaagaaaac agcattgaac
     8521 aagcgttttg ctgaatttga attaaaagca cttcaggcac aaatgaatcc gcattttttg
     8581 ttcaattgcc tcaataccat taagtttttt ataacaacca acaatcattc ggcagcaaat
     8641 aaatacttaa caaaattctc gaaattgtta cgtatgtttc tcgatcattc aacttccgag
     8701 agtatcacaa tcgaagagga aataaacatg ctaagacttt atatagaact tgagcaaatg
     8761 cgctttgatg aaggttttaa cttccatcta caagttgatg agagcattga ttataaaaac
     8821 actgagattc cggcaacaat acttcaacct tttgttgaga acgctattaa ccacggtttg
     8881 gtaaatttaa accgacaagg caatttaact cttatctttg agcaaaaaaa cggatgtata
     8941 gttggaatta ttgatgatga tggcatagga agagccgagg ccgcacgatt aaaaaaccat
     9001 tcaacatcac aacatatatc aagaggaaca caattgattg acgacagaat aaaaacgtta
     9061 aacagcattc gggaacagca tattgaaatt cagattatag acaaagaaga tgatcagcac
     9121 aatgccactg gtacacgtgt aatagttact ataccaattt aatacaaaac gagatgttac
     9181 aaacattaat agtagatgat gaaaaacgcg gccgcgagct gctaaaaatg attcttgcca
     9241 ccaactgccc tgaagttaaa atttctggcg aagcatcaaa tataaaagaa gcctatcaga
     9301 tgattcttca gaacgaaccc gatttggttc tgttggatat tgaaatgccc ggtggcacag
     9361 gtttcgattt actttctaaa tttgatgaag taaattttga tgttattttt attactgcat
     9421 ttgaccaata tgccataaaa gcaataaaat tttcggcaat ggattatatt ttaaaaccag
     9481 ttgacgaaga ggaattaatt aaagctatca aacgtgcaga gcaaaaccga aaccgtaaga
     9541 ataataaaga aaaagtaaac tcgctgattt caaatgttca gaagagcagc aagcatcaga
     9601 aaattggact gacttcgggt gaaggacttg agtttattga aataaaaaat attttacgtt
     9661 gcgaagctga tggaaaatac acttctgttt ttctgactga cggcaaaaaa ctattggtta
     9721 gtaaaaacct taaagagttt gaagacttac tcacagaata caatttcttc agaacgcacc
     9781 actcacattt agtaaacctc gactgtatta aaaaatacca aagtggtcgt ggtggctatg
     9841 tggtgatgag cgatggaagc accattacag tttcacaacg caagaaagac gactttttaa
     9901 gcagtctgaa gaaaatctga taattaagct tttgtcgaaa atacattttg ctgcttaggc
     9961 aacttagctt tactttcgca gcaaaattga gttacagtat gaaacacctc tttttagttt
    10021 tatgtattgc tgccactgca tttatatcat gtaagtccga tcagcaaaag aaaaccatag
    10081 aacctgaaat ggtaagcaac cctgcaagcg cagagcctgc aaatgcagac acttctcttt
    10141 tacccaaatt tgactttgaa agcgataatc acgaattcgg tcctattacc gaaggcgaaa
    10201 ttgtatctta caattttaag tttaaaaatt ccggtaaagc tccgttaatt attacacagg
    10261 caagtgccag ctgtggttgc accgttcctg aatattcaaa agatcctatt gctcccgggc
    10321 aggaaggatt tattaaagtc acctttaaca gcgaaggcaa acatggcatg acaagtaaga
    10381 cgataacttt acttgccaac accattccaa acacaaaagt tttaaccatt agtgcagata
    10441 tttctaaaaa agacaaatga tgtatttaac cattctacta cagtcgcaag gcaacatggc
    10501 catgcagcaa ttccttatgt tgggattaat tattgtaatc atgtatttct ttatgctgcg
    10561 gccacagatg aaaaaaatga aagttgaaaa gcaatttaaa gaaagtatta acaaaggtga
    10621 taaggtagtt accattggtg gcgtgcatgg aaaaattacc gaagttggcg aaactttttt
    10681 tatgattgag attgacaaca acgtaagaat taaaattgac aagacagcag tttcggcaga
    10741 agcatcaaag cagtatcgca aagttgaaga taaaaaataa gaagcctaac acttgaatat
    10801 ttttagaaat atcaggttta aaggcaatgc atcattgcct gtgtttttta tttgtgtctt
    10861 tgtgtccctt gttttctgga ttttatattc attgaataag aactatgtat atcccgtaac
    10921 ttatacagtt acacttcaga atactccatt cagtaaacaa gtcattagcg aaatacctca
    10981 acaggtaaat gtaacgcttc gttcaagagg ttttgagttg ttttggtatt taatcagaaa
    11041 ccgtgaaaga aacttaattg ttgacctgaa aaaatatacg gtaaacaatg aaaaattagg
    11101 cattagttta caaagcgaat tcagcaacca aaaccacagc agacttaaag aaatggatgt
    11161 tgtaactact ttacccgatt cggtgtggct atatctttcg tctaagtaca tgaaaacggt
    11221 tcccgtagtt acgcagttta cctattccat aaagaaaaac tttggcttgt caggaaaagt
    11281 cagcattgtg cccgacagca ttaccatttc gggagagaaa agtcagcttg atggtgtgaa
    11341 tgaaataaaa actgttgcag caagttacga caatcttgac cgtgacttaa aagctaccct
    11401 atcattaact ttacctgccg gcaaagttaa tttgtcgcaa aaagaaataa agattcatat
    11461 tcctgttgaa gaatttattg agaaaaaaat tcctattgct ttaaattatt ctatcaatgg
    11521 cagtggtaaa gttatactca ttcctgataa agcagaactc atcattcagg tacctttaaa
    11581 acgctatgag caacttaatg cagactcatt tcaggtaatg gtttcggcaa aacggtattt
    11641 aaaagaaggt caacttgcgg tagaattaat cagcaagcca caaggaataa atgtttcatc
    11701 aattattccg tcaaccgtaa actattatat agaacgttga tgaaaaaaat tggcataacg
    11761 ggtggcattg gctcaggaaa aacctttgtt gctaatattt ttaaaacttt aggcattcct
    11821 gtttatgatg cagacaatgc agcaaaacaa ttaatgaacc atgaaccgtt gcgcaacaag
    11881 ctcattcaat tatttggcaa cagtatttac aatgagcaag gcgaactcaa tcgtcctgtt
    11941 ttggcttcgc ttatttttaa tgataaaaaa aagttagatg cagtaaatgc cttggtgcat
    12001 cccgcagttg cacaacattt taatatatgg tgcaataaac aacaggctga ttatattctt
    12061 aaagaagctg ccattatgtt tgagtcggga gcaaataaga ttttagatgc tgtaatttgt
    12121 gttactgcac ctgaaacaac aaggattgaa cgcgccatga aacgcgataa cacatcagaa
    12181 gaaaaaataa aagcaattat tgccagacaa cttaatgaag aagatcgcaa caagcgctgt
    12241 aactatctga taaaaaatga tggcacggaa cctctattac cgcaggtgct gaaaattcat
    12301 gatttgttga aaggaaaata aaaaggccgt ctgaagcaga cagccctttt acctaaccta
    12361 aaactaacct gaaaaactcc ttattttctg tgttacggta attacaataa cgcaaatcaa
    12421 atgccactat tttaaatttt cgtgaatgat atatgaatgt tgcataactt tgacattatt
    12481 ctaaaaacga tgaaagaata tcaagcatcc cgaatgtcgg aagggaataa agtattgccc
    12541 aacaaaataa gaatagacaa tgatggtatt acattcagaa taccgggtgt attcagcggt
    12601 gaagaaaaaa ccattccatt ttcacgtata tcttctgttg aagtcaacac accgttggta
    12661 gggttttctt ctatcaccat aaacacaacc ggtgaaggcg ctattgtagc aacaggtttt
    12721 tataaagaag atgtgcttga aataaaaaaa atcgtactcg aaaaaattaa tattattcag
    12781 aataagtcat agcagcaact ggaaatacca tttgtacaat ctgagatatt aataaaatgg
    12841 tgaataagga ttcttttaat acagtaataa aagaataaag ttaaaaacaa ttgccgctgt
    12901 tttattacat ctgttatttg cagcgtgtaa aaagatagaa gcattaagat aaaatttatc
    12961 ctacctcatt tcagactctt taaccttact ctgtaccgta ctttccgctt gctgcaaggc
    13021 tgcattaaat gctttgtcat ctgacaatac aacacgataa aaagcatcgt tcccaaacaa
    13081 caaccttccg atgtttgctt taagctgatt ggcaattagc tgttttgatg cttgaatact
    13141 tttttcatcc ggtcttactt tattatcaat agcgtaaaca aaaaagtcgc tgagcaaacg
    13201 ctgatcggca gaaaatcctt ttatgtaatc atcaatactt ttaaaggtgt tgagtttgtt
    13261 cctgtgctta tcggtatagt cgtacgaaaa acgacttacc aatcctaatc caaaaacaga
    13321 acgggcaaat tcattgtcgg aagtggtgtc aataggaata aacaaatcgg gcataatacc
    13381 tcctccgcca tacactattt tacctccggg agtggtgtat ttaagtgagt cgttaaaatg
    13441 tgtactgtca gcatttacca tttcgccatg tttataccgg tcatacactt cttcttcgta
    13501 ggccatgtag ccattcttat aaggtttctg tatgctgcgt cctgttggtg tataataacg
    13561 tgcaacagtc agtcgcatgg ccgatccatc aggaaacagt gtttgctcct gtaccaatcc
    13621 ttttccgaaa gacctgcgac caataatttc tgccctgtcc caatcctgca aggcacctgc
    13681 aagaatttct gatgccgatg cagagccttc atctatcagc acaaaaacct ttcctttttc
    13741 aaaatcgcct ttctcagtag cataataatt ttttcgtggt cttgccttac cttctgtgta
    13801 aacaatcaat tttttatcag gcaaaaactc atcagcaagc atggttgctg catctaagta
    13861 accaccggga tttcctctta aatcaagaat aagattttgc attccctgtt ttttcaatgc
    13921 ctctacagcc tccataaatt catcgtaggt ggtggcagag aatctcgaaa ttttaataaa
    13981 gcctgtgttg gcatttgcca taaaagatgt ttctatactt cggataggaa ttttacctct
    14041 tactatggta aagtcgagca gcttctctat accactgcgt ttaactttca aggcaacctt
    14101 ggttccttct ggtccacgca gatacttaaa cacgtcttcg tttttaatgc caatgccggc
    14161 aacttttttc ttatctactt cgacaatgcg gtcaccgggt ctaaggccga tagtttcaga
    14221 agggccacct gcaatggttg acaccaccat gatagtatca tactgtatgt gaaactcaat
    14281 acctatacct tcaaaattcc cttcaagtgg ttcattaacc gacttcagtt catcggcagg
    14341 tatgtaggaa gaatgtgggt caaggttttg aagtatttgt gtaatactgc tgttgaccaa
    14401 ctcctgctga ttaatggtgt caacatactc ctgacggatg tagttaacca catcattaat
    14461 tttgttgaaa ccacccgatg cactgcggct gaacaaaata tttgtaccgc ctttaaatga
    14521 tgagtttaaa gaaacaccaa tgacaatacc tacggtaatc aacaaagcat accataatgg
    14581 ttgaaagttt tttttctgat cagactgcat gtaaaaatat tatgtaccgg aaacagttcc
    14641 gggggtatat tgttgcactt ctacattaaa ttttcttaaa aagtcaacac cttcatcgga
    14701 ttttatgcct ttatactgcg cataggaatt taaaaaaatc acttttttta ttcctgccgt
    14761 aaacaccacc ttggcgcatg ccagacaagg cgagagcgtt acatacagtg tagccccttc
    14821 taccgacact ttattttttg cagcataaag aacggcattt tgctcggcat gaatggctaa
    14881 ggagcatcct cccttgctgt cgcgcggaca accatcatca ggccattctg tatcgcaatt
    14941 gtgtgtgcct gcaggcggac cattatatcc gagagagatg atgcgtgtat ctttggtgag
    15001 taccgccccc acctgcattt taacacagtg cgattttaag gcaagtgtct ttgccaaatc
    15061 catataaata tcgtcaaaac cgggtgaacc catagctgtt tacattaaaa agcaaagccc
    15121 tcatgcgagg gctttttgta cccggagccg ggatcgaacc ggcatgaggt ttagctcact
    15181 ggtgtttgag accagcgcgt ctaccaattc cgccatccgg gtaaaattaa ttaattgcaa
    15241 tttttaaatg aacgtttctg tttatgttgc aattgatgat ttctaacttg cagcatttct
    15301 aaaaacgagg ctgcgaaaat agaaattttt aacttaaaca actgtgccat attaggaaaa
    15361 atcaatttcc taatatatat ttattggcga ggtctaaatt tcttccctta ttttattatg
    15421 aggaatcgca aaactgaact taatttaaac aacctcaata caatatccat ataatgactg
    15481 caataaaaaa caaactgtta taatacagta agaaaatact atttttaacc ttcattaatt
    15541 tataaaaact ctatgcagca attcatcaac aaatacactc taagtaaaac cctgcgtttt
    15601 gaactcaaac ctattggcaa aactttagaa aatattcaaa ccaaaggttt gctacagcaa
    15661 gatgctatta gagcaaaaag ctatcaagaa gtaaaaaaac tgatagaccg ctaccaccag
    15721 tattttattg aattggcttt ggatggtttg aagttgaata atttacagga ttttcaggag
    15781 caatatcttg catcaccaga agaaaagaaa aaagaggact ttaagaaaaa gtttgagaaa
    15841 aaccaagacg atctaagaaa agaaattgtg gctgctttta agacaggaga tgccaacgaa
    15901 atctttaaag ttctggataa aaaagaactg attacggagt tgatggttgc ttggctaacc
    15961 aaacaagagg ataaagatag aatagaggaa ttcaaaacct ttaccaccta ttttataggc
    16021 tttcatgaaa accgaaaaaa tatgtacacc gacaaggctc aaagcactgc tattgcttac
    16081 cgtttggtac atgaaaattt gccaaagttt ttggataata taaaggtgtt tgaaaaactg
    16141 aaagcagtac cggaactaca cgaaaagtgc agtgtactct ataaagagat tgaagaatat
    16201 ctaaacatac gctccataga tgaagctttt gagttagact attacaattc tgtcttaact
    16261 caaaaacaaa ttgaggtgta taacctcatt attggcggaa gagtggctaa agaaaatgaa
    16321 aagaaaattc aaggcttaaa cgaatacatc aacctataca atcaaccaaa agacaagaaa
    16381 aataaaatcc cgaaactgaa accgctctac aaaatgattt tgagcgatag ggaaagtgtt
    16441 tcatttttag ctgaaaagtt tgaaaatagt caagaggttt taaaggcaat tcaggaatat
    16501 taccacgcca atataaaatc ttatcaatcc tctgaaaaag atggagctga aaatgtattg
    16561 gaacgactac aggagttatt ggcaaactta agtagcaata atctttccca aatttatatc
    16621 cgtaacggaa aaaccattac tgatatttca caagctctgt ttggtgattt tagtttgata
    16681 aaagatgctt tgaaatatag ttttaccaac acgcttgaga taggtagaaa tggactttct
    16741 aagaagcagg aagaagtgat tgaaaaatac ctaaaacaag attatttctc tattacagaa
    16801 atagaagtag ccctttggaa ttatcgtact gaaaatgatt tacttaaaga cttggaagaa
    16861 gagcaacacc ttattgcaaa ctatttcaat agctatttca aaactaccgt caatgacaag
    16921 gaatatgatt tggtagctaa tgtaaccgca aaatatagtt gtatcaaagg gctattggaa
    16981 caggattatc ccgaagacaa aaaactgaat caagagacca aagaaattga tgatataaaa
    17041 gccttcttag atgctttgat gttggttttg cattttgtga aacccttaat gcttcctcac
    17101 gacagcacct tagaaaaaga ccaaagtttt tacaatacca tagcacctta ctatgaggaa
    17161 ttgcaatatt taatttcact ctacaacaag gtacgcaact ttgcttcgca aaaggcttat
    17221 agcgtagaaa agtataagtt gaattttgag aatagtactt tgttagccgg ttgggatgtg
    17281 aataaagagc ccgacaatac ttccgtttta tttagaaaag aaaatgatta ttttctaggt
    17341 gtgatggata aaaaacataa tggaatattt aagaaaacgc ctaagtctaa gacacaggat
    17401 acctattcca aagtcaatta caaattgctc ccgggtgcca gtaaaatgtt gcccaaagta
    17461 tttttcagta gaagcaatat tgcattttac aatccttcgg aagaaattat caccattaga
    17521 aaccatagca cacataccaa aggtggtaca ccacagactg gttttgaaaa gaaagatttc
    17581 aatctttcgg attgtagaaa gatgattgat ttctttaaag attccattgc caaacatccg
    17641 gattggaaac agtttggttt taatttttca gataccgata gctacgattc cattgatggt
    17701 ttttataggg aagtagaagc tcagggctat accatttcat acacagaaat agatgaaacc
    17761 tacatccatc aattggttga cgaaggcaaa ctctatctct tccaaatcta taacaaagac
    17821 ttttctgaat tcagcaaagg aaagcccaac atgcatactc tgtattggaa agccttattt
    17881 gattctgaaa atttgagaga tgtagtgtat aaactcaatg gacaagctga aatcttctat
    17941 cgtaaatctt ccatcaagaa ggataaaatt attgtgcata aagccaatga aaaggttgcc
    18001 aataaaaacc cgctcaacac caagaaggaa agtttgttta cttatgattt ggtgaaagac
    18061 aaacgattta cagtggataa atttcagttt catgtgccca ttacactcaa ttttaaagcc
    18121 agtggtaatg actacatcaa ccaagaggta ctttcgtttt taagacaaaa tcccgatgtc
    18181 aatatcattg gtttggatcg tggcgaaaga cacctcattt atctctccct catcaatcaa
    18241 aaaggagaaa ttttacaaca attttctctc aatgatatca tcaacgaata caaaggcatt
    18301 acccacaaaa cggcttacaa aacactttta gaaaagaaag agaaggaaag agctgatgcc
    18361 agagaaaact ggggaacgat tgaaagcata aaagaactga aagaaggcta tatctctcaa
    18421 gtggtgcata agatagccaa aatgatggtg gagtataatg ccattgtggt gatggaagac
    18481 ctgaacatgg gctttaaacg aggaaggttt gcggtagaaa agcaagtcta tcaaaagctg
    18541 gaaaaaatgc tgatagacaa gctcaattat ttagtgttta aagacaaagc tccgaatgaa
    18601 gcaggtggat tgtacaatgc cttacaattg gctaataaat ttgaaagctt tcaaaaaatg
    18661 ggtaaacaaa gtggtttctt gttttatgtg ccggcatgga ataccagtaa gatagaccct
    18721 accacaggtt ttgttaacct attttacact aaatacgaaa gcatagaaaa ggctcaaaag
    18781 ttttttgaaa aatttgatag cattcacttt aatacaaagg agcaatactt tgaatttgcc
    18841 tttgattacg acaatttcac cgaaagggca actggcacac aaacccaatg gacggtttgt
    18901 acccagggcg aaagaatttt aacctttaga aatccttctg cgaataacca atgggacaat
    18961 aaagaaatta agctcactga acaatttgaa gacttattag gcaaacacaa tatcagttat
    19021 ggaagtggga cctgcatcaa agcacaagta gctacacaaa gcggaaaaga cttcctaaaa
    19081 ggaatgatgg atttgtttaa actcaccctt caaatgcgta acagcattac gaacagtgaa
    19141 gtggattact tgatttctcc tgtaaggaat gctcaaggtt cattttacga cagccgcaat
    19201 gctgatgcga ctttacccaa agatgccgat gccaacggtg cttatcatat cgccaagaaa
    19261 ggattgatgt ggcttcacca aattcatgaa tttgaaggca atgattggag aaaactggat
    19321 ttggataaga ccaataaggg ttggttgaat tttgtgcaac ctaaaaacaa aactaatgag
    19381 taagaaaaaa gataaacaca ctccaaacat ccgcagttcg gcagccgagt atcttacttt
    19441 tatcgctgca acaggtgata atgagcaaag cgtagaaatg cgttatgagg atgaaaatat
    19501 atggctaaca caaaaaatgc tggcaatact ttatgatgtg gaagtaaaca cgataaacta
    19561 ccatatcaaa aagatatttg aagatgccga actagaagaa aattcagtta ttcgaaaatt
    19621 tcgaataact gctactgacg gtaaaaacta cgaaaccaat cactatggtt tacaaatggt
    19681 catgtctgta ggttttaaag taaattctga acgggcaaca caatttcgta aatgggtagg
    19741 gcaaatagct aaggattata ccatacaagg ttgggtaatg gatgttcctc gactgaagca
    19801 gagcggtact attcttacca aagaatactt tgaaaagcag ttagaaaaaa tccgtgagat
    19861 aagattgagc gaacgcagat tttatcaaaa aataaccgat ttatacgcta catcattaga
    19921 ctatgataaa agtgcgagta ccaccaaacg tttttatgct acagtgcaaa ataaattgca
    19981 ttgggcaatt catcggcata cagcggctga actaattgta aaaagagcca atcaccaaaa
    20041 agaaaatatg gggctaagca cttgggaagc tgcaccagaa ggtaaaattc aaaaatctga
    20101 cgtttccatt gccaaaaatt atctaactca aaatgaaatg gcatcgttgg aacgtattgt
    20161 ctctgcttat ttggatttag cggaagattt tacacttaga ggaataccaa tgaccatgca
    20221 ggattgggaa atacgcttga ataaattttt agaatttaat gaacgtgata ttttagataa
    20281 tcccggcaaa gtaacgcatg aaatagccca agcctttgca gaaagtgaat ttgaaaaatt
    20341 ccgtattgtg caggataaat tgtaccaatc cgactttgat aaattactgg aacatacaaa
    20401 aaacagaaaa ccttaaagca aattcacccc gaagttcttt gacatattaa aaagtaatgc
    20461 tgcttacgat atatcatgca acaaaaccgg aagccgattt tctaaattct tagaaaagta
    20521 tggtgtgcgt gtacaatttt ctgttttcga aattcaaaac tcaaatcgcg tattaaatat
    20581 tgtacttaat actattgaaa gaaaatttaa aaatagattt gatgcaggag acagtatcta
    20641 tatttttaaa gtaagccata aagaaactat ccgttatggg agtgctacat taatagataa
    20701 cgatcttatc atcatttaaa tatacaaaca taaggtaggt tctattcttg atttaagaca
    20761 acccactgaa tatcaattta gaatcagttc tcttcataaa aggtgggggg taactcccaa
    20821 ttttatcaaa gttgtaaaaa attgatattc aaagttaagt ttatactttt ggcagcgata
    20881 gaggcaaata agccaatcaa atttctacta ttgtagattt ccctgcctct attgcctgta
    20941 cctgtccggg caaataagcc aatcaaattt ctactattgt agattttcac atcattagac
    21001 aagcgattta cggcaaataa gccaatcaaa tttctactat tgtagatacg actctaagcc
    21061 atgacccttt aacggcaggc aaataagcca atcaaatttc tactattgta gatgaaacga
    21121 ataggttgaa tagttcttgg tggcaaataa gccaatcaaa tttctactat tgtagaatag
    21181 gcagcacagt atctcatttt taattgcaat gaaaaaataa tcaaaacgaa ctatttgaaa
    21241 atagcctaaa attccctttc tttgcagccc tttcagaaac tcgttattta tggcaagaga
    21301 agtcaaattg ttttccggtg aagctacacg ctatctggct gataacattg cacttagtta
    21361 tggtttgccg cttggtgatg ttattgtctc gcatttcagc gatggcgaat tttcgcccta
    21421 ttttgatgaa actgtgcgtg gttgcgatgt gtttatcatc caatctacct ttgcaccaac
    21481 caacaatttg tttgaacttt tgcttctgat agacgctgct aaaagggctt cggcacatta
    21541 tatcactgct gtaattccat actttggttt tgcccgcagc gaccgcaaag acaaacccag
    21601 agtagccatt gccagtaagt tagtagctaa cctgctcact gctgccggtg tcacccgtgt
    21661 gatgaccatg gacttacatg ctc
//
LOCUS       DAOQNP010000056        21050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_857_length_21050_cov_8.980948, whole genome shotgun sequence.
ACCESSION   DAOQNP010000056 DAOQNP010000000
VERSION     DAOQNP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 21050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21050
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_857_length_21050_cov_8.980948"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKY09_08575"
     CDS             <1..244
                     /locus_tag="PKY09_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12950.1"
                     /translation="QNAFAEYNQHLLQYNYYKSTALPNAETIISTATLSFNNGEINYL
                     EYAAALQNATTIKTSYLQSINEVNQSVININFLINQ"
     gene            285..650
                     /locus_tag="PKY09_08580"
     CDS             285..650
                     /locus_tag="PKY09_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNB12951.1"
                     /translation="MATYNFKDTAVYKKAFSLSMDIFEASKSFPKEETYSLTDQIRRS
                     SRSVCANLAEANRKKRYPANFISKLTDCDAENSETGVWLDFALDCKYITQVIYKTLVA
                     KNEEVGKLLNHMLNNPDKY"
     gene            720..1955
                     /locus_tag="PKY09_08585"
     CDS             720..1955
                     /locus_tag="PKY09_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB12952.1"
                     /translation="MKYQINKSSRTANCTLPIAFCLLLIASCLFLSSCGSKNNTTAHE
                     GEEHHDEHEHNLTTTLTAEQMKSIGIALGTLEKKQLTASLKANGILKVPNQNRANATA
                     SLGGTVKNILVQTGASVIKGQTIATIINNSFITLQEDYLSTAAKAELAKAEYDRQKIL
                     KEGNAASQKTFQQAEAELKSLQAKQSSLQKQLELIGISTQALTADNIQSTVSITSPIN
                     GSVSNVMVNIGTYVEANNPIAEIVDNSQLHLDLYVYEKDLSKLKEGQIIHFTLTNNPG
                     KEYDAKIYAISNTFEENTKAVAIHAKVEGNKQGLIDGMSITAIVSLENAMLDAVPTDA
                     IVSSQNQDYIIIVQNQESKPNTSGETTFEKIPVKKGTTDVGYSEITLLKDIPANSKVV
                     VNGAFFILAKMTNTGEHEH"
     gene            complement(2243..3541)
                     /locus_tag="PKY09_08590"
     CDS             complement(2243..3541)
                     /locus_tag="PKY09_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB12953.1"
                     /translation="MTLTFNATYSMRIFSSLILVFLFSVLAVEGKTVRALFIGNSYTD
                     VNNMPEIVKQIALSTGDTLIYSKNAPGGYTFQMHTTNATTISLIQAGNWDYVILQEQS
                     QLPSFPDNDVATQVYPYAAALDSMIKASSPCATTLFYMTWGRQNGDAANCQFFPPLCT
                     YLGMDSLLQLRYTIMAQDNHAAISPVAKVWRKIRSDFPTLNLYASDGSHPNNNGSFAA
                     ACSFYAVMFGKNPQLATYNYTVNTADASTIKTVAKEVAFDSLSFWIQYDALIADYNFS
                     ILSNTVSFFNSSQNATNYNWSFGDGNSSGQMNPAHTYSSPGTFQVTLIASDSVCSFSD
                     TITKAITVTTTGLLIPDSKNVFNIFPNPVFDMMTITSGKVISSFQIFSADGRLIFNTD
                     KPNLSNDEYSLNVSMLSSGIYLLKIYDDEHNTSCKKFTKS"
     gene            complement(3525..3935)
                     /gene="rnk"
                     /locus_tag="PKY09_08595"
     CDS             complement(3525..3935)
                     /gene="rnk"
                     /locus_tag="PKY09_08595"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNB12954.1"
                     /translation="MSKLIINRLDYARIKKSISDAKQFKSITNTEAEKLLQELDAAKI
                     VEPEAIPSNVVTMNSIVKLSFLNNDKNIQFQIVYPEQANIKENKISIFSPIATALIGY
                     KVSDEVEWIVPAGLTRIRIDEIIYQPEAAGDFDL"
     gene            complement(4046..4657)
                     /locus_tag="PKY09_08600"
     CDS             complement(4046..4657)
                     /locus_tag="PKY09_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNB12955.1"
                     /translation="MSKSDFDLNFQNKSVDGRIVAALERISQAFRVLLWQESRDYSLS
                     PIQIQVLIFLLHHSNDKCKVSYLADEFNMTKATISETVKTLEQKKLIQKNYQEYDNRS
                     YTIQLTKSGNAIATQTALFSKEFHKPIAKLNDDDKETMLLSLLNIINHLNKTGVITIQ
                     RMCLTCTHHEMSENEESHFCKLLNRKLHSTELRIDCPEHVLKM"
     gene            complement(4694..4957)
                     /locus_tag="PKY09_08605"
     CDS             complement(4694..4957)
                     /locus_tag="PKY09_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HNB12956.1"
                     /translation="MKVAVWDTYVTKKDGTIMHFDIIAPSDMKDTAVIYNYGKAFLKS
                     KGQEGQPLTAKECRFCHIETVKPNWQAAIEKSGYFIYEMENCN"
     gene            complement(5101..5334)
                     /locus_tag="PKY09_08610"
     CDS             complement(5101..5334)
                     /locus_tag="PKY09_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNB12957.1"
                     /translation="MSKSVFYHAGCPVCVSAEHDIVNLIGANNVEVVHIGENRSRIAE
                     AEGKGVKSVPALVTPAGHVLHINFGASMADVKG"
     gene            complement(5540..5734)
                     /locus_tag="PKY09_08615"
     CDS             complement(5540..5734)
                     /locus_tag="PKY09_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12958.1"
                     /translation="MKEFLRANWIVIAGTLTGAVGGYLYWKFVGCHDGSCAITSNALN
                     SSFYGAVVGGLFMSLFQKQP"
     gene            complement(5742..6308)
                     /locus_tag="PKY09_08620"
     CDS             complement(5742..6308)
                     /locus_tag="PKY09_08620"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNB12959.1"
                     /translation="MQKSVVILFLLFIALSSCQSGQKQKLTEQQKKEYVAKGDSISDF
                     MQGVLLKNVATAKNHGGTEFAIGFCNMNAIPITDSVAALYNVQIQRLSDKNRNPDNAI
                     KTLDDSLAWQKIKNYSSSFFEQQPSGNLVYYKPIKIGMPTCLKCHGSSADIESNTLAL
                     INEKYPEDRAVGYSQGELRGMWKIEFNQ"
     gene            complement(6312..6539)
                     /locus_tag="PKY09_08625"
     CDS             complement(6312..6539)
                     /locus_tag="PKY09_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12960.1"
                     /translation="MNKVFSGWNWMRIIRLALGIFIVFQGVQDKSWLIATLGILFTVM
                     AVFNAGCCYARSCSMPADNADKTQIENSAKI"
     gene            complement(6634..7260)
                     /locus_tag="PKY09_08630"
     CDS             complement(6634..7260)
                     /locus_tag="PKY09_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNB12961.1"
                     /translation="MQNSNTEPDAKNSQVNDPSKQSFWNERWETKQTGWDIGYASPAI
                     AQYFSKFSDKEAAILIPGCGNAYEAEFLLNEGFKNITLVDIAPKAVEILRNKFAGNSN
                     VRILCVDFFTIGEKFDILIEQTFFCAIPPGKRKAYVQKAADVLNSEGKIVGLLFDTMF
                     EKEGPPFGGSRIEYEQFFNPLFNVMMIPCERSITPRAGTELFIELQKK"
     gene            complement(7250..7567)
                     /locus_tag="PKY09_08635"
     CDS             complement(7250..7567)
                     /locus_tag="PKY09_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNB12962.1"
                     /translation="MIGLIKNMLGMENTQLTEAIKQGAFLIDVRTPAEFAGGSAAGAV
                     NIPLDKIGSQLSKIKNKSKVVVFCKSGMRSMQAKNILEQNGIINVINGGTVNDIIRIQ
                     NAK"
     gene            complement(7605..9020)
                     /locus_tag="PKY09_08640"
     CDS             complement(7605..9020)
                     /locus_tag="PKY09_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB12963.1"
                     /translation="MFFQHVYDKSLAQGSYFIGCQAKGEAIVIDAQRDIDVYLEIARQ
                     NNLKITHITETHIHADFLCGSRELAAVTGAKMYLSNEGGKDWQYEFNHEGVKDGDVIT
                     VGNLTLKVMHTPGHTPESISFLLTDHPASDKPVMVFTGDFVFVGDIGRPDLLEKAAGI
                     KGTKEVGAKQMFESLKKFEALPEYVQVWSAHGAGSACGKALGAVHSSTVGYEKIRNWA
                     FQYGNDEQGFVDYLLADQPEPPKYFAMMKHLNKVNRPLLVEVPKHAKLSKEEFLSAYN
                     KGIAVIDTRNKFEFAEGFLPGSLNIQGNNSFSTWMGWLLNYQQQFILVADDKTIVNLT
                     RKLMRIGMDNMLGYVTDVASFGIELEKSEVINLSKFKSTINNDNYQIIDVRGLTEFEA
                     GHVEGAEHIFVGTLAENLNKINKKKQVVIHCQAGDRSAIAFSLLHKHGFKNVWNYAGG
                     MKEWVACNEATITERQMACAI"
     gene            complement(9126..9557)
                     /locus_tag="PKY09_08645"
     CDS             complement(9126..9557)
                     /locus_tag="PKY09_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB12964.1"
                     /translation="MSQTHFYEVNVQWKEGRIGELSSPILEKTIECATPPEFPNGVPN
                     IWSPEHLFVAAINSCYMATYLAIASNFKVELEDFSCRTVAKLEMVDGKYLITQAELFP
                     KIKLKDSNDAEKAQRIADKSKAGCLVTNSMKTEILMTTKIS"
     gene            complement(9745..10140)
                     /locus_tag="PKY09_08650"
     CDS             complement(9745..10140)
                     /locus_tag="PKY09_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB12965.1"
                     /translation="MEIVKSHIGSENYKVTIESQSGNILIADEPVANGGKNLGLSPFE
                     LVAAALSACTSITVRMYAERKKWPLSDVNTDISVSWDKLNNKTVIQRKVNFSGNLSDE
                     QKNRLLQIANVCPVHKVLTNPIEIFTEMF"
     gene            complement(10237..10854)
                     /locus_tag="PKY09_08655"
     CDS             complement(10237..10854)
                     /locus_tag="PKY09_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNB12966.1"
                     /translation="MELLKIIDIAGIAVFSISGVFAAMEKKLDVFGVFIIAFITALGG
                     GTLRDVLIGQLPVSWMYNLNYGLIVLLSTLAAMFFSNIIGNYQKTLLTFDSLGLGLFT
                     VVGIQKGILLDFHPAVCIALGTITACFGGVIRDILLNNIPLIFQKEVYATACILGGVV
                     YFVLMRFQMNEMITEMVSITFIVVFRLVAVRFNWQLPSIYKIQKK"
     gene            complement(10861..11166)
                     /locus_tag="PKY09_08660"
     CDS             complement(10861..11166)
                     /locus_tag="PKY09_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HNB12967.1"
                     /translation="MVSYMPTLSERMNELRLKGYKEDFNIQCDGIDSGNGCIKLSPDD
                     FHIDAYYRFEGQSDPADEAVLYAISSERYGLKGLLVNGYGVYSDDMTNEMLKKLSFK"
     gene            complement(11252..11917)
                     /locus_tag="PKY09_08665"
     CDS             complement(11252..11917)
                     /locus_tag="PKY09_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNB12968.1"
                     /translation="MAEHLKIIAIEKETHDVLHITTEKPENIKYHAGQAADISINKPK
                     WENELRAFTFTSLPTDKQLEFTIKTYPQHNGVTNQLLTLKTGDTLLLHGVFGTIAYKG
                     EGLFIAGGAGITPFIAIFKQLERDGKVGDNKLLFANKTHADIIQEKRYKALLGKNFIN
                     ILSEEKLEGFEYGYISEGLLKQHLNATGYVYLCGPEPMMNAVEKQISNLGIKKEQVVK
                     EVF"
     gene            complement(12212..14086)
                     /locus_tag="PKY09_08670"
     CDS             complement(12212..14086)
                     /locus_tag="PKY09_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNB12969.1"
                     /translation="MLNRGIPISVSLEPTLKTDSSEKVKYDKRRLFKLSLLAILISVC
                     ISVIAKFLIKLIDLITNISFFGHYSVEDVSPAHNNLGLLVIVVPAIGGLIVGLIALYG
                     SKAIRGHGIPEAMEQILTNQSKIKPSITFLKPLSSAISIGTGGPFGAEGPIIATGGAL
                     GSTMGQLMRITPNERKILLAAGATAGMAAIFGSPVAGILLAIELLLFEFSPRSFVPVA
                     LACITGSAGHHIFFGSGVVFPMAATIPDSSSYALLFYSAMGIIIGVLAVATTKIVYWI
                     EDSFEKLPIHWAWWPAIGGVAVGIVGYFEPRTLGVGYNNITDLLSGSLTLQVAVSLCV
                     LKFISWAISLGSGTSGGTLAPLLTIGGGLGVMLGFVGNQWFPAAEISIPLAALIGMSA
                     MFAGASRAFLTSIVFALETTGQSNALLPLLASCTASYFISYFLMENTIMTEKIARRGV
                     YTPSTYEPDILEKIRVEQLATDNGITISDDMKLGEVRNWLQQEPDLQANYYIISSTDG
                     RYIGLLSSTSIFSSHHPSDTLIGSLVKRTQVFVSKSDSLKMAVQTMAKENVDILPVVT
                     EKEIVGVLTYHHIISVYNHGIDEHEKKQKHISLKRQRLKILLRGQKLVNILRTRNHEE
                     "
     gene            complement(14432..14809)
                     /locus_tag="PKY09_08675"
     CDS             complement(14432..14809)
                     /locus_tag="PKY09_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12970.1"
                     /translation="MKLYLILLYVLLVLQPLAAQKKPCVNSFDSTAVIEYIKGKGWID
                     LVPAKTLVKLNYIPEVVSFANINSETCRWKVISQTSYHSNEGDCHFTNGCTVVVKQTI
                     VLSARNKRLISRRKSIKFYPNYE"
     gene            15019..16158
                     /locus_tag="PKY09_08680"
     CDS             15019..16158
                     /locus_tag="PKY09_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNB12971.1"
                     /translation="MLFQLNEEHLMIQKAARDFAQNELLPGVIERDEHQKFPAEQIKK
                     LGELGFLGMMVDPKYGGSGLDTISYVLAMEEISKVDASVSVVMSVNNSLVCWGLETFG
                     TEEQKQKYLVPLAKGEIIGAFCLSEPEAGSDATSQRTTAIDKGDHYLLNGTKNWITNG
                     GTASVYLVMAQTDVAKGHKGINCLIVEKGTPGFTVGPKENKLGIRGSDTHSLMFTDVK
                     VPKANRIGEDGFGFKFAMKTLAGGRIGIASQALGIASGAYELALKYSKERKAFGKEIS
                     QHQAIQFKLADMATEIEAARLLCLKAAWLKDQHGNYDLAGSMAKLYASKVAMDTTVEA
                     VQVHGGYGYVKEFHVERLMRDAKITQIYEGTSEIQKIVIARAVTS"
     gene            16162..17403
                     /gene="pepT"
                     /locus_tag="PKY09_08685"
     CDS             16162..17403
                     /gene="pepT"
                     /locus_tag="PKY09_08685"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNB12972.1"
                     /translation="MLNNYQHTTAERMMRYAVIDTTANPDSDTQPSSKKQLDLSRLLV
                     SELKAMGIDVELDEYGYVYATIKGNSKKKVPVLCFCSHVDTAPDCSGANVKPILHKKW
                     NGEDIVLPDDKNVVISSKKHPYLLEKIGDDIITASGFTLLGADDKAGISIIMDFAQYL
                     LNRTDIIHGDIRILFTPDEEIGRGVNKVDMKKLNADFGYTLDGGECGTLEGENFSADS
                     CTVTFNGISAHPGYAKGKMVNAQKVLAHFITLLPNEMSPENTEAYQGFVHPVSISGGL
                     EAATVTFILRDFVTSKLSEQKQLLNNLAEQTIKQFPGAGFTISSKEQYRNMDEVLQKH
                     PEVMDNACEAFKRSGINCERKNIRGGTDGARLSFMGMPCPNIFTGEMGIHSKEEYVSV
                     QDMNKAVEVLVNLVQVWEQRA"
     gene            17650..19710
                     /locus_tag="PKY09_08690"
     CDS             17650..19710
                     /locus_tag="PKY09_08690"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNB12973.1"
                     /translation="MKKQVTLLFGIMCFALAVNAQYYYFPHINAGQNPGGLNTDDEYP
                     VGGGQPAGWTSIHPGSATTPQWSTTVTIPFSFSFNGAAVTSYKVSTTGVLTFTTGAAT
                     PPPVANVALPSALVPDNSVCCWGISGSGTNDNICTKTFGTAPNRQHWIAFTSYTDPGN
                     NAYTYYSIVLEESTNKIYVVDQRNGGTALTLTIGVQINATTAVSVAGSPNIGQNAGSD
                     PSPADNSYYEFIYGSQPANDAALSSLNYTQYVTTPTNIPVSGVITNLGSSAINTIELK
                     YELNGTTYTDTKTGLNIAPLGTYNFTHATPINANAIGTYSFKFWVDLTGDVNHINDSL
                     NGVINYLPFLPTKRVVFEEATGTWCGWCPRGAVFMDSLRNLHGQEVMLIAVHNGDPMT
                     VAAYDSWMGTKISGYPSGLVDRKDLDVDPSDFITEYNSLISDIPPCDVNVTATYNTST
                     NVGTFTVSPQFAASLTGDYRINCVVTEDNLTGTTSTWSQANYYSYQTANLPLQGAGHN
                     WQNETDPVPAANMEYDHVARAILGGNDGVQGSLPANITSGGTYSWTFNYNIPATQNVS
                     NLIIIGWVSDFATGRILNANSATVTTGIHNLTGTYFNMNITPNPITSDYGQVRVNLKK
                     NADVVFEIIDVLGNIVRTNYQSQMSSGEYVYPVNFTGLSNGIYTVRMNASGETLTQKV
                     VLNR"
     gene            complement(19820..20902)
                     /gene="aroC"
                     /locus_tag="PKY09_08695"
     CDS             complement(19820..20902)
                     /gene="aroC"
                     /locus_tag="PKY09_08695"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNB12974.1"
                     /translation="MAGNTFGTLFRITTFGESHGPLIGVVIDGCYSGVPLDLDLIRTQ
                     LSRRRPGQSAITTQRMEDDEPEIVSGVINGITTGTPLTIIIRNKDQRPVDYDVLRDAY
                     RPSHADYTYEKKYCIRDHRGSGRASARETAARVAAGAVAMQMLKVVNISVNAYVSRVG
                     NVSIPENTSIDTTQTYNNILRCPHEATALLMQRKIEETAQTGDTLGGIISCIISGVPA
                     GLGEPVFDKFHAQLGKAMLSINAVKGFEIGSGFFGSSQTGSQQNDVFETDKNGKVFTT
                     TNNSGGVQGGITNGMDITFNVAFKPVSTLMKSQQSINTQKQKVIIQPGGRHDVCVVPR
                     ATPIVEAMAAITMLDFLMIANAAKLH"
BASE COUNT         6862 a         4085 c         3549 g         6554 t
ORIGIN      
        1 gcaaaatgct tttgcagaat acaatcagca tttgctgcaa tacaattatt acaagtcaac
       61 cgcattgccc aatgcagaaa caattatcag cacggcaaca ctttcattca acaatggaga
      121 aataaactat ctggagtatg cagccgcttt gcagaatgca acaacaatca aaacaagtta
      181 tctgcaaagc ataaacgaag taaatcagtc agtcataaat atcaatttct taataaatca
      241 ataaacaatt tgcaataggc tatttgcaat aaactcaaac taaaatggca acatacaatt
      301 tcaaagacac cgcagtttac aagaaagcgt tttctctttc aatggatatt tttgaagcca
      361 gtaaatcatt tccaaaagaa gaaacttatt ctcttacaga ccagattagg agaagttcaa
      421 gaagtgtttg tgcaaatctt gcagaagcaa atcgtaagaa aagataccct gctaatttca
      481 tttcaaaact taccgattgc gatgctgaga attccgaaac aggcgtatgg cttgacttcg
      541 ccttagattg caaatacatc acgcaggtga tttataaaac actggttgca aaaaacgaag
      601 aagtaggaaa gcttttgaat cacatgctta acaatcccga taaatattga aatcaatatg
      661 caataagcga taagcaagtt gcatggaaaa ttaaacaaca ttttataaca aaatcaaaaa
      721 tgaaatatca aataaataaa tcatcaagaa ctgcaaattg cacattgccc attgcttttt
      781 gcctattgct tattgcctct tgcctatttt tgtcttcctg tggaagcaaa aacaatacaa
      841 cagcacatga aggcgaagaa catcatgacg aacatgagca caatttaacg accacactaa
      901 ctgccgaaca aatgaaatct atcggcattg cgttgggaac attagaaaaa aaacaactaa
      961 ctgcttcact caaagcaaat ggtattttaa aagtgcctaa tcaaaaccgt gcaaatgcta
     1021 ctgcatcact tggcggaacg gtaaaaaata tcttggtgca gactggtgca agcgtcatca
     1081 aagggcaaac cattgcaacc atcatcaaca actctttcat caccttgcag gaagattatt
     1141 taagtacagc agcaaaagcc gaattggcaa aagcggaata tgacaggcag aaaattttaa
     1201 aagaaggaaa tgcagcatcg caaaagacat ttcagcaagc cgaagccgaa ttgaaatctc
     1261 tgcaagcaaa acaatcaagc ttacaaaaac agttggagct gattggtatc agcactcaag
     1321 cactcacagc cgacaatatt caaagcaccg tcagcattac aagccccatc aatggatcag
     1381 tcagcaatgt gatggtcaac ataggaactt atgttgaagc caacaatcct attgccgaaa
     1441 tagttgacaa cagtcagtta catttggatt tgtatgtata tgaaaaagat ttaagtaaac
     1501 tcaaagaagg tcaaataatc catttcacac tgaccaacaa ccccggcaaa gaatatgatg
     1561 caaagattta cgccatttca aacacctttg aagaaaatac aaaagccgtt gcaattcatg
     1621 caaaggtgga aggcaacaag cagggattga ttgacggcat gagtatcacc gccattgtga
     1681 gtttggaaaa tgccatgttg gatgctgtac ctaccgatgc aattgtcagt tcacaaaatc
     1741 aagactacat tatcattgtg caaaatcagg agagcaaacc taacacatca ggcgaaacaa
     1801 cttttgaaaa aattcctgtc aagaaaggaa caactgatgt gggctacagt gaaattactt
     1861 tactcaaaga cattccagca aacagcaaag tagttgtcaa cggagcattt ttcatccttg
     1921 caaaaatgac aaacactggc gaacatgaac attaaaatca ctttcgggtc attcctcttc
     1981 acttcacctt taggtttgta ccgacaaatt ttaataatgg catctgtcag aaaaatactc
     2041 aaataacaag ggtgaactat catcgcacat aggtttcaag acagcatcaa aaaggaaagt
     2101 caacaacact atagagaaag aagagtggct catataataa agtattgcca aatgtgttac
     2161 ggaaatgctt cgattggacc tttaaactta gacctaagct atagcgcaac aacggtcaag
     2221 ttttaaacaa acattgtttt gtctaagact tagtaaactt tttacatgat gtattatgtt
     2281 catcatcgta tattttcaac aaatatatac ctgaagaaag cattgacaca ttcaaactgt
     2341 attcatcatt agacaaatta ggtttatctg tattgaaaat caatctgcca tctgctgaaa
     2401 aaatttggaa agaggaaatt actttgcctg atgtaattgt catcatatca aaaaccggat
     2461 taggaaaaat attgaataca tttttactat caggaattaa taaacctgta gttgtcactg
     2521 taattgcttt tgtaatagta tctgaaaaac tacaaacaga atcagatgca atcaatgtaa
     2581 cttgaaatgt tccgggcgat gaataagtat gtgcaggatt catctgtcca ctactattgc
     2641 catcaccaaa tgaccaatta tagttagttg cattttgcga actgttaaag aagctaactg
     2701 tattgctaag tatagaaaaa ttatagtcag caatcaatgc atcatactga atccaaaaac
     2761 ttaagctatc aaatgctact tctttagcaa ctgttttaat tgtggaagca tctgctgtgt
     2821 taactgtata attatatgtt gccaactgtg gatttttccc aaacataaca gcgtaaaaac
     2881 tgcatgcagc agcaaatgat ccattattgt ttggatgact gccatcactt gcataaagat
     2941 tcaatgttgg aaaatctgat ctgattttac gccagacttt agctacgggt gaaatagctg
     3001 catggttatc ctgtgccatg atggtatatc ttaactgtaa caaactgtcc attccaagat
     3061 aagtacatag tggtggaaaa aactggcaat tggcagcatc accattttgc cttccccagg
     3121 tcatgtaaaa aagtgtagtt gcacaagggc tacttgcttt tatcatactg tctaaagctg
     3181 ctgcataagg ataaacctgt gtggctacat cattatccgg aaatgatgga agctgacttt
     3241 gctcctgaag aataacgtag tcccaatttc cggcttgaat taagctgata gtggttgcat
     3301 tggtagtatg catttgaaat gtatatccac cgggtgcatt cttactatat atcagtgtgt
     3361 caccggtaga aagcgcaatt tgtttcacta tctcaggcat gttatttaca tcagtataac
     3421 tgttgccaat aaataatgct ctcacagttt ttccttcaac agcaagcaca gaaaataaga
     3481 aaactaaaat taaacttgaa aatattctca tggaataagt agcattaaag gtcaaagtca
     3541 cctgcagctt ccggttgata tataatttca tcaatgcgaa ttcttgttaa tcctgcaggg
     3601 acaatccatt ccacttcatc actaacttta taaccaatga gcgctgtagc aatgggtgaa
     3661 aaaatagaaa ttttattctc cttaatattt gcttgctcag gatacactat ttgaaactga
     3721 atgtttttgt cattattcaa aaaactcaat ttcacaatag agttcatagt aaccacatta
     3781 gaaggtattg cttccggctc aacaatttta gctgcatcca attcctgcaa taacttttct
     3841 gcttctgtat tggtgattga cttgaattgc tttgcatcac ttattgattt tttaatgcgg
     3901 gcataatcca aacgattaat tatcagctta ctcataaatt ttgaaatata aaattaaggt
     3961 gcaaagataa ggtattccaa aatattggat tctgataaag actgaaaaat acctgttttg
     4021 tgtaataaaa cgcctattat tgtatctaca tctttaatac atgttcagga cagtcaatac
     4081 gaagttctgt agaatgtagt ttacggttta gtaatttaca gaaatgactt tcttcatttt
     4141 ctgacatttc atgatgtgtg caagttaagc acattcgttg aatagtgatg actcctgttt
     4201 tgttgagatg attaataata tttagaaggc tcaatagcat tgtttcttta tcatcatcat
     4261 tgagttttgc tattggctta tgaaactcct tgctaaataa cgctgtttgt gttgcaattg
     4321 catttccaga ctttgttaat tgaattgtat agcttcggtt atcatattcc tgataatttt
     4381 tttgaataag cttcttttgc tccagagttt ttactgtttc actgatggta gccttagtca
     4441 tattaaactc atctgccaga tagcttacct tgcatttatc attactgtga tgaagcaaaa
     4501 aaattaatac ctgaatttgt attgggctca aagaataatc tctgctttct tgccaaagta
     4561 aaacacgaaa tgcctgtgag attctttcaa gggctgcaac aatacgtccg tcaacacttt
     4621 tattctgaaa gttaaggtca aaatctgatt tactcattgg tgaattatta ttcaggagaa
     4681 aaatacaaca ttcttagttg caattttcca tttcataaat aaaataacca cttttttcaa
     4741 tagctgcttg ccaattgggt ttcactgttt caatatggca aaacctgcac tctttagctg
     4801 tgagtggctg cccttcctga ccttttgatt taagaaatgc tttaccataa ttataaataa
     4861 ctgccgtatc tttcatgtca gaaggtgcaa tgatgtcaaa atgcattatt gttccatctt
     4921 tcttcgttac ataggtatcc catactgcta ctttcatagt ttttgatgtt tttgtttgtg
     4981 ctgtcaacaa tgcaggtaca gacacaagaa ttaatactat tattgtcttt attgatttca
     5041 tttataaaaa gaaaatttac cccgcaaagg tagttatcct aactatacgg ggcaaatttt
     5101 ttaacctttc acatcagcca ttgatgctcc aaaattaatg tgcaacacat gacctgctgg
     5161 tgttaccaac gcaggtactg actttacgcc tttaccttct gcctcagcta tacggctacg
     5221 gttttctcca atgtgaacta cttcaacatt gttggctcca attaaattta caatgtcgtg
     5281 ctctgcacta acgcatacag gacatcctgc atgataaaaa actgatttac tcattttgtt
     5341 tgtgttatga tgttatttgg cattattacc gctgcaaata tagttaggtt tcctaactat
     5401 tacaaacttt tctaataatt attaatattt catcttaact actaatttaa aaaaagcgct
     5461 aatagaagtt gaaaatagat ttgactatca gccaagaagc ttcaacccat ttataaaaaa
     5521 agaaatccct taaaaataat tatggctgtt tttgaaaaag tgacataaag agtccgccaa
     5581 ctaccgcacc ataaaatgaa ctgttcaatg catttgaagt aatggcacat gagccatcat
     5641 gacagcctac aaacttccaa tataaataac ctccgacagc tcctgtaagc gtaccggcaa
     5701 taacaatcca atttgcgcgt aaaaattctt tcatgtttaa attattggtt aaattcaatt
     5761 ttccacattc ctcttaactc accttggcta tatcctactg ctctatcctc aggatatttt
     5821 tcatttataa gtgcaagggt gttagattca atatctgcac tactaccgtg acattttaag
     5881 caggtgggca ttccgatttt aataggtttg taatacacta agtttcctga aggctgttgt
     5941 tcgaaaaaac ttgatgaata gttttttatt ttttgccatg ccaaactatc atccaacgtt
     6001 ttaattgcat tatccggatt cctgttctta tcactcaatc gttgaatttg aacattgtat
     6061 agtgcagcaa cagaatctgt aattggtatg gcattcatat tgcaaaaccc gattgcaaat
     6121 tccgtaccac catgattttt tgctgtggct acattcttca ataagacacc ttgcataaaa
     6181 tctgaaatac tgtcgccttt agcgacatac tctttctttt gttgctcagt aagcttttgt
     6241 ttttgtccgc tttgacatga gcttaaagca ataaataaca gaaataaaat tacaacactc
     6301 ttttgcatca cttatatttt tgctgaattt tctatttgtg ttttatcagc gttatctgct
     6361 ggcatcgaac aacttcttgc ataacaacag cctgcattaa aaactgccat caccgtaaat
     6421 aatatgccca atgtagctat tagccaactt ttgtcctgta cgccttgaaa gacaataaat
     6481 attcccaaag ccaaccgtat tatgcgcatc caattccatc ctgaaaaaac tttattcata
     6541 ctattttaaa ttaaacaatt agaattttca tattacttca gtgctttctt acactaaact
     6601 tacatgatat aagaacccga aattttaaac caactatttt ttttgtaatt ctataaacaa
     6661 ctctgttccc gcacgcggag ttatacttct ttcacaggga atcatcatta cattgaataa
     6721 aggattaaaa aactgttcgt attctattct gcttcctcca aatggtggtc cttctttttc
     6781 aaacattgta tcaaacaata atccaacgat ttttccttca gaatttaaaa catctgcggc
     6841 cttttgaaca tacgctttac gtttgcccgg aggaattgca caaaaaaatg tttgctcaat
     6901 taaaatatca aacttctctc ctatcgtaaa aaaatcaaca caaagaattc ttacatttga
     6961 attcccggca aacttattcc ttaatatttc tacagccttt ggagcaatgt ccaccaaggt
     7021 aatgttttta aaaccttcat tcaataaaaa ctctgcttca taagcgttgc cgcatccggg
     7081 tattagaata gcggcctctt tatcagaaaa cttactaaaa tattgtgcta ttgccggaga
     7141 cgcataacct atgtcccatc cggtttgctt tgtttcccat ctttcattcc aaaaggattg
     7201 ctttgacggg tcgtttacct gagaattttt cgcatcaggt tctgtgttgc tattttgcat
     7261 tttggattct gataatgtca ttaacggtac caccattgat aacatttata attccatttt
     7321 gttcaagaat gttctttgcc tgcatactac gcataccact tttgcaaaac accacaactt
     7381 ttgacttatt tttaatcttt gacaactgac ttcctatttt atccaatgga atatttactg
     7441 cacccgcggc actgccacct gcaaactctg ctggagtacg tacatcaata agaaatgcac
     7501 cttgtttaat tgcttctgtt agttgtgtat tttccatacc caacatgttt ttaattaatc
     7561 caatcatttt atttgaagtt aactgttagt aatttacatt gttattaaat agcgcaagcc
     7621 atttgccgct cagtgattgt agcttcatta catgctaccc actccttcat gccaccggca
     7681 tagttccaca cattcttaaa accatgttta tgcaacagag aaaaagcaat tgctgaacgg
     7741 tctcctgcct gacaatgaat aaccacttgt ttcttcttat taatcttatt aagattttct
     7801 gccaaggttc caacaaaaat gtgttctgcc ccttcaacat gtcctgcttc gaattctgtt
     7861 aaaccgcgca catcaatgat ctgatagttg tcgttattga ttgtgctttt gaattttgat
     7921 aaattaatca cctctgattt ttccagttca ataccgaatg aagctacatc agttacataa
     7981 ccaagcatat tatccattcc aatacgcatg agttttctgg ttaaattaac tatagtttta
     8041 tcatctgcaa ctaaaataaa ctgctgctga taattaagta accatcccat ccaggttgag
     8101 aaagaattat ttccctgaat atttaaactt ccgggcaaga agccttctgc gaattcaaat
     8161 ttatttctgg tatcaatcac ggctatcccc ttgttgtaag cagagaggaa ttcttcttta
     8221 gacaacttag catgcttagg cacttcaact aaaagcggac gattcacttt attaagatgc
     8281 ttcatcattg caaaatactt tggcggttca ggttggtcag ctaatagata atcaacaaag
     8341 ccttgctcgt cattaccata ttgaaatgcc caattacgga ttttttcata tcctacagtt
     8401 gaactgtgaa cggcacctaa tgccttacca caagctgagc ctgcaccatg tgcagaccat
     8461 acctgaacat actccggtaa tgcttcaaat tttttcagag actcaaacat ttgtttggca
     8521 ccaacctcct tagttccttt tatacctgct gctttctcta acaaatcggg gcggccaata
     8581 tcaccaacaa agacaaagtc acctgtaaaa accattacag gcttatctga tgccggatga
     8641 tctgtcagca aaaagctaat actttccgga gtatggcccg gagtgtgcat cacctttaaa
     8701 gtaaggttac caacagtaat tacatcaccg tcttttacac cttcatgatt aaattcatac
     8761 tgccagtctt taccaccttc gttagataaa tacatctttg ctcctgtaac tgcagcaagc
     8821 tcacgcgagc cacataaaaa gtcagcatga atatgtgttt cagttatgtg agttatcttt
     8881 aagttatttt gtcttgcaat ttcaagataa acatcaatgt cgcgctgtgc atcaattaca
     8941 atagcttcac cttttgcctg acagcctatg aaatagcttc cctgtgctaa acttttgtcg
     9001 taaacgtgtt gaaaaaacat atagttataa tttaaatgtt atagttgtta tttttttaaa
     9061 agtatttgaa ttttatactg caaagtaagt aacgatttct caaacccttt gttactcatg
     9121 ttacattaac tgatttttgt tgtcattaaa atttctgtct tcattgaatt ggttaccaaa
     9181 cagcctgcct ttgacttatc ggcaatgcgt tgtgcttttt cagcgtcatt gctatctttc
     9241 agttttattt ttggaaatag ttctgcctga gttatcaaat attttccatc taccatttcg
     9301 agtttggcaa ctgtgcgaca actgaaatct tccagttcta ccttaaagtt tgatgctatt
     9361 gccagatagg ttgccatata gcaactgttg attgcagcaa caaataaatg ctccggtgac
     9421 caaatgtttg gaacgccatt aggaaattca ggtggtgtgg cgcattcaat tgttttttca
     9481 agtatgggtg atgataactc ccctattcta ccttctttcc attgtacatt tacttcgtaa
     9541 aaatgtgttt gtgacatatc tgttgttttt aaattttagt gaactaatca atgtgtaaaa
     9601 tgtcattcta tatgacagtg caaaagtgcc tatacccaac aatttttgct gtgacataag
     9661 ttacattaaa gtttactttt cgctaaaaat taaatgagat atatcatgtt ttttttaaaa
     9721 cagattaata acagactaat ttcactaaaa catctcagtg aaaatctcaa tgggattggt
     9781 aagcacttta tgaaccggac aaacattggc aatttgcagc aatcggttct tctgctcatc
     9841 tgaaaggtta cctgaaaaat taaccttacg ttgaataact gttttgttat tgagtttatc
     9901 ccagctaaca gagatatcag tatttacatc agaaagtggc catttttttc tttcggcata
     9961 catccgcact gttattgaag tgcatgcact caatgctgca gcaactaact caaatggtga
    10021 aagaccaaga tttttgccac cattcgctac cggctcatca gcaataagaa tatttcctga
    10081 ctgcgattca atcgttactt tataattctc gcttccaata tgtgatttta ctatttccat
    10141 aactcaaatt ttataaatat ctttcatttc aagtgggctt gctaattaat gaacacatgt
    10201 actcatttca acattattca gatcagtaat cagaatttat ttcttttgaa tcttgtaaat
    10261 agaaggcaac tgccaattaa accttacagc caccagtctg aaaacaacaa taaacgtaat
    10321 actaaccatt tcagtaatca tttcattcat ctgaaaccgc atcaggacaa aataaaccac
    10381 accacctaaa atacatgcag tagcataaac ttctttttga aaaattaatg gaatattgtt
    10441 aagcaaaata tcgcgaatta ctcctccgaa acatgcagta atagttccca atgcaatgca
    10501 aacagcagga tgaaaatcaa gcaatattcc tttctgaatc ccgaccacag taaacaatcc
    10561 taatccgagt gagtcgaacg tcagtaatgt tttttgataa ttaccgatga tgttagagaa
    10621 aaacattgct gcaagcgtac ttaacaacac tatcaaacca taatttaagt tatacatcca
    10681 tgatacaggt agttgtccga ttaatacatc gcgcaaggtt cccccaccta atgctgttat
    10741 aaaagcaatt ataaaaacac cgaatacatc gagtttcttt tccatagcag caaatacccc
    10801 tgatatacta aatacagcaa taccggcaat atcaattatt tttagaagtt ccaaaaaggt
    10861 ctatttaaaa ctcagtttct ttaacatctc attagtcatg tcatcagaat aaactccata
    10921 gccatttacc aataaacctt taagtccgta ccgctctgat gaaatagcat ataatactgc
    10981 ttcgtctgcc gggtctgact gtccttcgaa acgatagtag gcatctatat gaaaatcgtc
    11041 aggcgataac ttaatgcatc cgttgccaga atcaatgcca tcgcattgaa tattaaaatc
    11101 ttctttataa cctttcaacc gcaactcatt catgcgttca gaaagtgttg gcatgtaact
    11161 taccataatt ttataaaata atattgttaa agatacagtt tctgtttata ttttcaaaaa
    11221 aagaattgac aacattacta tttaacatga tttaaaacac ctcttttact acttgctctt
    11281 tttttattcc caggttgctt atctgttttt ctactgcatt catcataggt tccggtccac
    11341 agagataaac ataacctgtt gcatttaaat gctgcttcaa cagtccttct gaaatatagc
    11401 catattcaaa cccttcaagt ttttcttctg aaagaatatt tataaaattc ttgccaagca
    11461 aagctttgta tctcttttcc tgaataatat cagcatgagt tttattggca aacaacagtt
    11521 tgttatcccc aaccttacca tccctttcaa gttgtttgaa aatcgcaata aagggcgtta
    11581 tgccggcacc accggcaata aaaagtcctt ctcctttata tgcaattgta ccaaagacac
    11641 catgtaaaag caacgtatca ccggtcttta aagtaagcag ttgattggtt acaccattat
    11701 gctgcggata tgttttaatg gtaaactcta actgtttgtc tgttggtaaa gaagtaaaag
    11761 taaatgctct aagctcattt tcccatttag gcttgttgat tgaaatatct gcagcttgcc
    11821 cggcatggta ttttatattt tccggtttct ctgtggtgat atgaagtaca tcgtgtgttt
    11881 ctttttcaat tgcaattatc tttaaatgtt ctgccataac ttttctttat tttatttatt
    11941 ggtcatttag aattgcttat tttaataact cataatacag actaaacata ataagtaata
    12001 atttgtttta aagcaaatat gttgcacgaa acagcatatc aaaagtttgc tttcaacaat
    12061 tctttgagcg tgctaatcgc aaagaagaat aaaaaaacta attcagtttc cgcctctgtg
    12121 atttctcatc attagctaaa agttgtactg cctgttaatc atttctatac ttacattaaa
    12181 caatgaaaat cagcaaaaaa acaactgtaa tttattcctc atggtttctt gtacgcagaa
    12241 tattcacaag tttctgacct cttaggagaa ttttcaaacg ctgacgtttt aaagagatat
    12301 gtttctgttt tttttcatgc tcatcaatac catgattgta aaccgaaatg atgtgatggt
    12361 aagttaatac tcctacaatc tctttctctg tcaccacagg caaaatatct acattttcct
    12421 ttgccattgt ttgtactgcc attttaagag aatcggattt gctgacaaaa acctgcgtcc
    12481 gtttcaccaa agaaccgata agtgtatcag atggatgatg cgaactgaaa atacttgttg
    12541 agcttagcaa accaatatac cttccatcgg tacttgagat gatataataa tttgcctgca
    12601 agtcgggttc ttgttgaagc cagtttctga cctcacccaa cttcatatca tcactaatag
    12661 ttatgccatt atctgtagca agttgttcta ctctgatttt ttctaaaata tccggctcgt
    12721 atgtacttgg tgtatatact cctcttcgtg caattttttc tgtcatgatg gtgttttcca
    12781 tcaggaaata agaaataaaa taagatgccg tacaagaagc caataaaggt aacaatgcat
    12841 ttgactgacc agttgtttca agtgcaaata caattgaagt aagaaaagcg cgtgaagccc
    12901 ctgcaaacat agcagacata cctatgagtg ctgcaagtgg aatacttatt tcagcagccg
    12961 gaaaccattg attgcctaca aagccaagca tcacacctaa accaccacct atagttaaca
    13021 atggcgcaag tgttccaccg gatgttccgc tgcctaaaga aatagcccat gaaataaatt
    13081 tcaaaacaca taatgaaaca gctacctgta atgttaatga acctgaaagc aaatcggtaa
    13141 tgttgttata gccaacacct aacgtacgtg gctcgaagta acccactatg cctactgcaa
    13201 ctccaccaat ggctggccac catgcccaat gtataggaag tttttcgaat gaatcttcaa
    13261 tccagtaaac aatttttgta gtcgccactg ccaaaacacc tataatgatt cccattgccg
    13321 aataaaacaa caatgcatat gaagaagaat caggtattgt agcagccatt gggaaaacca
    13381 caccactgcc aaagaaaata tgatgtccgg cagagcctgt tatgcatgcc aatgccactg
    13441 gcacaaaaga gcgtggagaa aattcaaaca acagaagttc tatggccaat aaaatgcctg
    13501 caacgggact tccaaaaatt gctgccatgc ctgctgttgc tcctgctgcc aatagaattt
    13561 ttctttcatt gggagtaata cgcatgagtt gacccatggt tgaacccaat gcaccacctg
    13621 ttgcaataat aggtccttcc gccccaaatg ggcctcctgt tccaatagaa atagctgatg
    13681 atagcggctt taaaaatgtt atggatggtt taatcttact ttggttagtt aggatttgct
    13741 ccattgcctc aggaattcca tgaccacgaa tggcttttga gccatagagc gcaatcagac
    13801 ctacaattaa accaccaata gcaggtacaa caattacaag taaccccaga ttgttatgtg
    13861 caggactaac atcttccaca ctatagtgtc caaaaaagga aatattggta attaaatcaa
    13921 taagtttaat cagaaactta gctattacac tgatacaaac agaaattaat attgccaaca
    13981 atgacaattt aaaaagtcgt cttttatcat actttacttt ctctgaagaa tcggttttta
    14041 aagtaggttc taatgaaacc gaaataggta ttcctctatt caacatctgt aaaatgtgtt
    14101 ttagtgaagt ttaattgact tacaggaaaa tcacaacaca ttacaagatc attcttttga
    14161 agtataacgt ttgacttaca actttgaatg attactatga tttgatttga atgcaacaaa
    14221 ctcaaggtta aaataatatt tgagcaaaat agaaaataca agaaaagata tataagttca
    14281 gagtcgttat tcagtttcct gaaacaatac tttctaaaat gcaacagatg caaaattata
    14341 catgatggct gaaaatatta tgttaaaaaa ttagaatcag atgatgtttt attaacagag
    14401 caatctctta tgtttaactt gacaagtcta tttactcgta attcggataa aattttatac
    14461 tttttctcct actgattaaa cgtttatttc gtgcacttaa tacaattgtt tgctttacaa
    14521 ctacagtaca accgttagta aaatggcaat caccctcatt agaatgatat gaggtttgag
    14581 atataacttt ccatctgcag gtttcactat ttatatttgc aaaagataca acttcaggaa
    14641 tgtaattaag cttaaccagg gttttagccg gcactaagtc tatccatcct ttacctttaa
    14701 tatattctat aacagcagta ctgtcaaaac tatttacgca tggtttcttc tgtgcagcaa
    14761 gaggctgtaa taccaaaaga acatatagca aaatgagata taatttcaat tttattcgaa
    14821 ttttaatttg aaggaatatt tgtataaaag tagcattgta gaaataatca cattgtcttt
    14881 tgcgataaaa aggcagctcg ctaaggtcgc aaagaaaacc ctttatttat agcattcgac
    14941 tacgcctgca ctgtcacaca agaacttcct attttcaaat tcgtgccgaa gttgtacatt
    15001 cgcaaaaatt ttcagaatat gctatttcaa ctaaacgaag aacatttaat gatacagaaa
    15061 gcagcccgtg attttgcaca aaacgagttg ttacccggag ttattgagcg cgatgaacat
    15121 cagaaatttc ctgcagagca gattaaaaaa ttaggtgagt taggcttttt aggtatgatg
    15181 gttgacccca aatatggcgg atcaggatta gacactattt cctatgtatt ggccatggaa
    15241 gaaatatcta aagtagatgc ttctgtttca gttgtcatga gtgtaaacaa ctctcttgtt
    15301 tgctggggtt tagaaacttt tggcacagaa gaacagaaac aaaaatattt ggttccatta
    15361 gcaaaaggag aaataattgg cgcattttgt ttgagtgaac ctgaagctgg cagcgatgcc
    15421 acttcacaac gcaccacagc aatagacaaa ggtgatcatt acctgctgaa tggaacaaag
    15481 aactggatta ccaacggagg cacagcaagt gtctatttag tgatggcaca aactgatgtt
    15541 gcaaaagggc ataaaggtat aaactgtctg atagttgaaa aaggtactcc cggttttaca
    15601 gtcggaccaa aagagaataa attaggtatc agaggcagtg acacacactc actgatgttt
    15661 actgacgtga aagttccaaa ggctaaccgt attggcgaag atggctttgg atttaagttt
    15721 gcaatgaaga ctttagcagg tgggcgcatt ggaatcgcct cacaggcatt aggtattgca
    15781 tccggtgctt atgaactggc tttaaaatat tctaaagagc gtaaggcctt cggaaaagaa
    15841 atcagccaac atcaggcaat tcaatttaaa ttagccgaca tggcaacaga aattgaagct
    15901 gcaagactac tttgtttgaa agctgcatgg cttaaagatc aacatggaaa ttatgatctg
    15961 gccggaagta tggcaaaact gtatgcttca aaagtagcaa tggacacaac cgttgaagct
    16021 gttcaggtgc atggcggcta tggttatgta aaagagtttc atgttgaacg cctgatgcgc
    16081 gatgctaaaa ttacgcagat ttacgaaggc acttccgaaa tacaaaagat cgtaattgcc
    16141 cgtgctgtta cttcataaga aatgttaaac aactatcaac atactactgc tgagcgaatg
    16201 atgcgttatg cagtcattga caccacagcc aatcctgatt cagatacaca gcccagcagc
    16261 aaaaagcaat tagacttaag caggttactt gtgtctgaat taaaagcaat gggtattgat
    16321 gttgagcttg atgaatatgg ttatgtgtat gctacaatta aaggtaactc aaagaagaaa
    16381 gtacctgtac tctgtttttg ttcgcatgtt gacactgcac ctgattgcag cggggcaaac
    16441 gtaaagccta tcctgcataa aaaatggaat ggcgaggata ttgttttgcc ggacgataaa
    16501 aatgttgtta tcagcagtaa aaaacaccca tatctactcg aaaaaattgg tgacgatata
    16561 ataacggctt caggatttac gctgttaggt gccgatgaca aagcaggcat ttctatcatc
    16621 atggattttg cacagtatct tttaaacaga acggatatta tacatggtga tattcgtatt
    16681 ttatttacgc ctgacgaaga gataggtcgt ggtgtaaaca aagtggacat gaaaaagctg
    16741 aatgctgact ttggttatac actagatggt ggagaatgtg gcacattaga aggtgaaaac
    16801 ttttcggctg actcatgtac ggtaacattt aatggcatca gtgcacatcc cggttatgca
    16861 aaaggtaaaa tggttaatgc acaaaaagta ttagcacatt tcatcacact attaccgaat
    16921 gaaatgagtc ctgaaaacac agaagcgtat caaggctttg tacatcctgt cagcattagt
    16981 ggtggattag aagcagcaac agtaactttt attcttcgcg actttgtaac ctcaaagtta
    17041 tccgaacaaa agcaactgtt aaacaattta gcagagcaga caatcaaaca attcccagga
    17101 gcaggtttta ccatcagtag taaagaacaa taccgtaaca tggatgaagt acttcaaaaa
    17161 catccggagg taatggacaa tgcatgtgag gcatttaaac gcagtggcat caactgtgag
    17221 cgaaaaaata tcagaggcgg gacagatggc gcacgccttt catttatggg catgccttgt
    17281 ccgaatattt ttacgggaga aatgggaatt cacagtaaag aagaatatgt aagcgttcag
    17341 gatatgaata aagctgttga agtgttggtt aatctggttc aggtttggga acaacgagcc
    17401 tgatagtagc actttcctac aattccattc aataaatata actcaggagc tcagcaaagt
    17461 tctaactaag ttagttgtaa attggtttgc tattacaatg caatcacatt agcagtacaa
    17521 acattggaat attaacttta aacaaacgct aaaggcttgt tttgtattaa aacaaatatt
    17581 taactaaata gcctaaaaac tatgttccga aaaatgatag ttttgctatt aattaaatta
    17641 aaataaaaaa tgaaaaaaca agttactctt ctattcggca taatgtgttt tgcattagct
    17701 gtaaatgcac agtattatta ttttccgcat atcaatgcag ggcaaaatcc cggtggactt
    17761 aacactgatg atgaatatcc ggtaggtggc ggtcaacctg caggttggac atccattcat
    17821 cccggaagtg ctaccactcc acaatggtca acaactgtaa caattccgtt ttctttctct
    17881 tttaacggtg ctgcagttac aagttacaaa gtatctacaa caggcgtatt aacatttaca
    17941 acaggtgcag ctacacctcc accggttgcc aatgttgctt taccatcagc tttagtacct
    18001 gacaattctg tttgttgttg gggaatttca ggaagtggta caaatgataa tatttgtaca
    18061 aaaacatttg gaacagcacc aaaccgtcag cattggattg cattcacatc ctacaccgat
    18121 ccgggcaata atgcttatac ttattatagt attgtgttag aagaatcaac aaataaaatt
    18181 tatgtggttg atcaaagaaa tggtggaaca gcattaacac tcactattgg tgttcagatt
    18241 aacgcaacaa cagccgtttc tgttgcgggt tctcctaaca ttggacaaaa tgccggatca
    18301 gatccatcac ctgctgacaa cagttactat gaatttattt atggatcaca gcctgccaat
    18361 gatgcagctt tatcctcttt aaactataca caatatgtaa caacgccaac taacattccg
    18421 gtatcaggtg ttattactaa tctcggctca agtgcaataa atacaataga actaaaatac
    18481 gaactcaatg gcactactta taccgacacc aaaactggat taaatattgc tccccttgga
    18541 acatataatt ttactcatgc tactccgata aatgcaaatg caataggaac atattctttc
    18601 aaattctggg tagatttaac cggagatgta aatcatatta atgatagctt aaatggagtc
    18661 attaactatc ttccattttt gccaaccaag cgtgtagtat ttgaagaagc cacaggcacc
    18721 tggtgcggat ggtgcccacg cggtgctgtt ttcatggatt ctcttcgcaa cttacatggt
    18781 caggaggtta tgttgatagc tgttcataat ggcgacccaa tgactgttgc tgcttatgat
    18841 tcatggatgg gaactaaaat tagtggctat ccaagtgggt tagttgatcg taaagattta
    18901 gatgttgacc cttcagattt tattactgaa tacaacagcc ttatctccga tattccacct
    18961 tgtgatgtaa atgtgacagc aacatacaat acaagtacta atgtcggaac gtttacagta
    19021 agtccacaat ttgctgcatc attgactggt gattatagaa ttaattgtgt agttacagaa
    19081 gataatttaa ccggcacaac ttctacttgg agtcaggcaa actattatag ctaccaaaca
    19141 gcaaatctgc cattacaagg tgcagggcat aactggcaaa atgaaaccga tccggtacct
    19201 gcagccaaca tggaatacga tcatgttgca cgtgcaattc ttggtggaaa tgatggtgta
    19261 caaggttcat tgcctgcaaa cataacttcc ggtggtactt acagctggac atttaattat
    19321 aatattccgg caactcaaaa tgtaagcaat ttgattatta ttggatgggt ttctgatttt
    19381 gctacaggca gaattttaaa tgcaaacagt gcaacagtta ccacaggcat tcataactta
    19441 acaggaactt atttcaatat gaacataact ccaaatccga taacttctga ttatggtcag
    19501 gtgagagtta atttgaagaa aaacgcagat gtagtattcg aaattattga tgttttagga
    19561 aatattgttc gtaccaatta tcaatcacaa atgtcttccg gtgaatatgt ttatcctgta
    19621 aatttcacag gtctaagtaa tggcatttat acagttagaa tgaatgcatc cggtgaaacg
    19681 ttaacacaaa aagttgtatt gaatagataa aaacaacccg gtagatttac cgtgtttatc
    19741 ttaaaataaa aaggcagttt taatctaaga attgaaactg cctttttatt ttaacgctaa
    19801 ttcaattcaa cttaatttat taatgcaact tagcagcatt ggcaatcatt aaaaaatcga
    19861 gcatagtaat tgcagccata gcctcaacaa ttggtgttgc gcgtggcact acacaaacat
    19921 catggcgccc tcccggttga ataatcacct tctgcttttg ggtattaatg ctttgttgcg
    19981 acttcataag cgttgagact ggcttaaatg ctacgttaaa agtaatgtcc ataccattgg
    20041 taattccacc ttgcacacct ccggagttgt tggttgtagt aaaaactttt ccatttttat
    20101 cagtttcaaa aacatcgttt tgttgcgacc cagtttgtga agatccaaaa aatccggaac
    20161 ctatttcaaa gcctttaaca gcattaatac ttagcatggc cttaccaagc tgtgcatgaa
    20221 atttatcgaa tacaggctca cctaaccctg caggaacacc actaataata caggatataa
    20281 ttcctcctaa agtatctcca gtctgtgcag tttcttcaat ttttctttgc atcaacaagg
    20341 cagtggcctc atgtggacaa cgcaaaatat tgttataggt ttgtgtagta tcaatggaag
    20401 tattttcagg aatggaaaca ttaccgactc tacttacata ggcattaaca gaaatgttta
    20461 caacttttag catttgcata gcaacggcac ctgcagccac acgtgcagct gtttcgcgtg
    20521 ctgaagctct tccacttcca cggtgatcgc gaatgcaata ctttttttca taggtataat
    20581 ctgcatgcga tggtctataa gcatccctta gcacatcata atctacagga cgttgatctt
    20641 tgttgcgaat aataatagtt agtggagtac cggtagttat gccattaatt actcctgaaa
    20701 caatttccgg ttcatcatct tccatccgtt gtgtggttat tgcagattga cccggcctgc
    20761 gccttgacag ttgtgttctt atcagatcca aatccagtgg tactccagaa taacaaccgt
    20821 ctatgacaac accgataagc ggtccatgtg attcgccaaa agtggttatc ctgaaaagtg
    20881 ttccaaaagt atttcctgcc atattacttt ttaatcatta caaaatcgtt cataaaatag
    20941 ccgttgccta tatcaaaatc ttctgcacgt tcaataacaa aacctttttt aaaataaaag
    21001 ttgatagctt tataatttga acggttaacc tgcaaacgta ttacaatatt
//
LOCUS       DAOQNP010000057        20997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_864_length_20997_cov_7.861857, whole genome shotgun sequence.
ACCESSION   DAOQNP010000057 DAOQNP010000000
VERSION     DAOQNP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20997
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_864_length_20997_cov_7.861857"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1163
                     /locus_tag="PKY09_08700"
     CDS             90..1163
                     /locus_tag="PKY09_08700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB12975.1"
                     /translation="MFDESVKYKGVGENENNYLAQKILLSEKLELLDNMALIGGYDEK
                     KFLESVDSAATLKNELLKGFVLEKEFAAAEKKDIEFAQLDNIASYEMYHRYIKHNEDF
                     KVSESYPDAFKNFNFNDESSVKNELYLKLLSDFFSKQAYKQIREDSTRNFLMEFSNAV
                     LERVQSTIVRNGLGRLIVEDGLKSGNDLKVSYAAIKKLISDKDVQASLDEKFAMLSKL
                     QKGNTSPDFSLQDIKGKTFSLSDFKGKVVYIDVWATWCGPCKAEMPFMKKIQEDLKGK
                     DVAFIGICAWDKQENWKKYIEENKMQGTQLFAPDRALPFLKEYDIKGIPRFILLDKEG
                     KIVEADAARPSDPQLKAKIESLL"
     gene            complement(1233..1748)
                     /locus_tag="PKY09_08705"
     CDS             complement(1233..1748)
                     /locus_tag="PKY09_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNB12976.1"
                     /translation="MNKPLHHFFTTDHHRLEKLLDKAIITIPEIDMTFYMPFRSGLLR
                     HIKMEENILFPAAKAIDANKMKEILPQFRLEHGALTALMVPPPTPDLIKVIKHILEKH
                     DFEEEKPGGLYDICELLTHAQTQNLLQQLANTPEVPLHPPNPTTFAFDAAKRALIRAG
                     YDYDVIVRSES"
     gene            1856..3868
                     /gene="ligA"
                     /locus_tag="PKY09_08710"
     CDS             1856..3868
                     /gene="ligA"
                     /locus_tag="PKY09_08710"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNB12977.1"
                     /translation="MSKISEKEATEKIKQLTVDLNHHNYLYYVKAQPVITDYEFDVML
                     QELIALENQFPELRSSESPSQRVGGYISKEFPTVTHNYPMMSLGNTYSEEELRDFDER
                     IRRVIGSNFEYVCELKFDGVAIGLTYRNGKLLRAVTRGDGVRGDDVTNNVRTIRSIPL
                     CLMGNNFPDEFEIRGEIILDKKTFERINNERADIGETPFANPRNSAAGTLKLQDSSEV
                     ARRKLDCFLYALYGDFDFSTHYDGLKMAAEWGFKISSHYRLCDSIENVFQFIETWRQK
                     RDSLPFDIDGIVIKVNNFRQQEELGFTAKSPKWAIAYKYKAESATTILNEVVYQVGRT
                     GAITPVANLKPVLLAGTTVKRASLHNADQIQKLDLHLHDTVFVEKGGEIIPKITGVDL
                     SMRKAGAIAVKYITHCPDCGAELIRQEGEANHYCPNENGCPPQIKGRLVHFTSRKAMN
                     IDGLGVETIDQLVEAGLLKNVADIYHLNSDDLLQLERMGEKTVGNLFEAIEKSKQVPF
                     ERVLFAIGIRFVGDTTAKKLARHFGSYHKISQATLEELMLTPEVGDKIAASIFDFVRQ
                     PANINMVAQLQESGLLFELDASSVRDAGTKLKGLSFVITGVFQKFERDKLKETIEING
                     GKVSGSLSSKTSYLIAGSDCGPAKLEKAEKLKINIISEDDFLKMLQ"
     gene            3865..4419
                     /locus_tag="PKY09_08715"
     CDS             3865..4419
                     /locus_tag="PKY09_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12978.1"
                     /translation="MKIGKAIKQKAASYLFYRELQRQHHQPRTTSFSNARTIGILYDA
                     TIERNYEIVKAYVKHLRDDFKKDVLALGYYDANELPPMRFSKLGLDFFTKKNLNWHFK
                     PSHPMISKFINLDFDYLICLNVDRCMPLRYISAATKAGFKIGRYEKGYAGLYDFMISV
                     NGQVTLPQFIDHVNNYLKHIGHDK"
     gene            4416..5291
                     /gene="dapA"
                     /locus_tag="PKY09_08720"
     CDS             4416..5291
                     /gene="dapA"
                     /locus_tag="PKY09_08720"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNB12979.1"
                     /translation="MNKFSGTGVALVTPFLKSGKTDFDALRKVVNHVIKGGVDYLVVM
                     GTTGESVTLSKEEKKDILKFIGEVNKKRTHIVFGVGGNNTLETARELEHIDDQMADAI
                     LSVSPYYNKPNQSGIVQHYKTLSGASQLPIILYNVPGRTGSNLLPQTTLEIAASCKNI
                     IGIKEASGNMEQIMYLIHHRNKGFLVISGDDAITMPLIASGADGVISVIANAYPKQFS
                     TMVRLALQGKMVESRKLHYQLLDLIPLLFAEGSPSGIKYVLSQMGICQNYFRLPVAPV
                     SKTLAQKIDKVIKEI"
     gene            5318..6334
                     /locus_tag="PKY09_08725"
     CDS             5318..6334
                     /locus_tag="PKY09_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNB12980.1"
                     /translation="MGHPAHFHLFKNTVVALQQAGHEVQILIKKKDILEDLLQHAGMQ
                     YININPEGRRDDKFSIALKLLKRDFQFFKICLRFKPQIMIGTSAEIAHVGKLLGIRSI
                     VVNEDDAEVVPWFAKLAYPWATNILAPHCCSVGKWEHKKIGYKGYHELAYLHPAQFSP
                     QKENIKNLGDEPFFILRFAKLTAHHDEGRKGINTLIAEKLINILSKHGRIYITSEREL
                     EPQFEQFRIAINPLHIHDALYFAQMYIGDSQTMAAEAAVLGTPAIRFNDFVGQISYLE
                     ELEKEFALTFGFKTNDADAMLTHVESLLNQQDLKQHWQEKRNQMLNQCIDLNLFMIKM
                     LTQQ"
     gene            6470..7582
                     /locus_tag="PKY09_08730"
     CDS             6470..7582
                     /locus_tag="PKY09_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNB12981.1"
                     /translation="MIFKTDNTEAKLLQLEEFAELDVVLAAEAKNNVQNERFVVNLIE
                     KYVDTSRSDCFTFAANNHDDANQLADDTYRAIIHFKRVNNFHFINKQFEAINTKLPKG
                     GIFVSKVETNDLRKQRLMEKYPPYFNKVYYFFDFIFKRVFPKLPVTQQIYYAITKGHN
                     RVLSKTEMLGRLYSCGFKVLEERYIGNRLYIVSEKFREPAYDEDPSYGIIYPMKRVGK
                     GGKIISVYKFRTMHAYAEYLQEYIYEKNNLEEGGKFKDDFRVSRVGRMLRKYWIDELP
                     MILNVLKGDLKIVGVRPLSRHYLSLYSKELQEMRKKFKPGLIPPYYVDLPKTMEEIMD
                     SEMRYLKAYQKNPFLTDVRYFLMVFYTILFKRARSK"
     gene            7642..9285
                     /locus_tag="PKY09_08735"
     CDS             7642..9285
                     /locus_tag="PKY09_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12982.1"
                     /translation="MKVIRLIFILILIVAFQLNSSAQYVPQSVFNKPLYDFMDDLANL
                     HIITLNDAIKPYSRKQIAEALQAAESQKEKLNTTQQKQLAFYLKDFNKELSTTKGFKK
                     RLDLFYFKDSLFNITVNPVGGFSYFKNENDNMYRQYAGGEFWATLGKGLSIFGSYRDY
                     TDSRAVADDKYLNDLPGYNYKYKGAVGSPKRGGSFDETRGGIAYAWKWGHIGIVKDNL
                     EWGSNNHGANIISTKAPSFARLELKLNPKKWFEFNYFHGWLYSEIKDDSAAYFSGNPP
                     NHRQVYFSKYFAANMFTVKPFKQFDFSFGNSIVYSDQLEVAMFIPFMFFKSLDHSKTG
                     QGSNYSGQNSQLFFNISSHNIKYTHLYISLFVDEIAFGRMMDKDEHSNFYSLKAGTAV
                     MLPFYTSATIVAEYTRTNPVTYRHFVNTTTYESNRFNMGHYLRDNAQEIYLGARCTPI
                     SGLYISAGYTTAAVGPIYPYTGKDKSGLGLPFLKTKEYEFTSLDLNIKYELFNDIFLS
                     AGVKIDDNKGALANVYTAPFYYGKNGKTTTLFGGFNIGF"
     gene            9288..9812
                     /locus_tag="PKY09_08740"
     CDS             9288..9812
                     /locus_tag="PKY09_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB12983.1"
                     /translation="MNFDNLTTALLIIAPTFIVAGGMYMLIKNMMERDYRLKLLEARR
                     LIQKDMLPLKLQAVERMVVFLERIQPENILFRILQPGMNVRDLQVDLLAAIRQEYEHN
                     VSQQVYVSVQSWSLITKAKDDIIATVNLAADSLDKTEKAIVLSQIILQRSSAGNNFAG
                     AAILSLKSEVDSFL"
     gene            9846..10847
                     /locus_tag="PKY09_08745"
     CDS             9846..10847
                     /locus_tag="PKY09_08745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNB12984.1"
                     /translation="MSFSTRIIFKTAVKLQLQNLLKLIPVSPNGVTILCFHRISSQYD
                     YFWQPIYPETFGLMLESLVKEYQIIPFSQIDNTFDKNGKPPLVLSFDDGYKDFLEEAI
                     PLLQFYEVPSNHNIVIDCADGKNYIWTQKLNHLFNLLRNSSRFSIHVALDKHFILDIT
                     QPCKKWFVHYYRTLFYLFKLDAKARAECISVLEQYLNIAENDYPIKMMNWDDIKFLSL
                     NNVEIGCHTTSHVSLPSVENDEEFFLELDYAKKRIEEELKCECPVIALPNGLTNNLVN
                     QKIADAGFKKILCLAGSGSNASKSTDKVKFYNRLNMIQEPVRYMNVRIKFYEKGFLR"
     gene            10844..11815
                     /locus_tag="PKY09_08750"
     CDS             10844..11815
                     /locus_tag="PKY09_08750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB12985.1"
                     /translation="MSDLQLRQLQNNELNLLPKLMQDCFNMKVDEDYFKWKYLNNPAG
                     NFIGFGAFSNNSLVGFKGVFPGLYNVRGTQTIIYQSGDAMTHPDFRRQRLFEKLATSC
                     FEKIKEKHQLIVIGFGGTKSTPGLIKIGWTHLADVKFFFIPKLKCRWNSLFSFRLKHY
                     QCNETSDLNEIVTLYNKYGVKKDITPIYSNEFLTWRLSNPRFKYHCVVCENSNERLGF
                     LIYYQDGDKIFILDYCGKNDKTLSALFNYVSKVCIEKKLKGLITIAIPKSSEGKQLKR
                     CGFLQNPFQAGPMSAKIPFIVLTEPSLKEHFLHQNQWLVIPICHDAL"
     gene            11946..>13759
                     /locus_tag="PKY09_08755"
     CDS             11946..>13759
                     /locus_tag="PKY09_08755"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HNB12986.1"
                     /translation="MKKISTILMAACLVVGLANGQSQRYVVVEEFTGETCGPCASNNP
                     GFNAKLNANSYTLPIKYQNNIPSNGPNFYAYNTTDIGNRTSYYANNYSPHAFLDGNVW
                     NGNAGSFPVATLNSRVAATSPFTIDVTHSFSPTHDIIYVHAVIRATQAVTGLSSLKAR
                     IAITEKDNYGYTSPNGESHYSDVMRKMLPNGTGTVLPATWAVGDSVVLDEQWTINVPS
                     NIPTVFLPYWPMLRAVVFVQNDANKEILQGGRSTVAAVVPLAAITNLSTMFSCTSSLD
                     PAVTLTNYGGGGNLTSAVIEFGITGQAMQTYNWSGTLAAGASTIITLPNMTLPGGGMQ
                     NFSAKLTSINGNPDYYNINLNFSAPVAQAAAPTTSFSQDFYSTVFPPVNWILNNVDNG
                     FTWTRSAVGGFQMNPGGSARMNFYNSANGQVDELLPYTPVDFTNAVNPQLTFSVAYCQ
                     YSNENDRLDVRVSTDCGATWSSVYNKAGAALSTTAAQTTSFTPSSASQWRAETVSLAN
                     YVGQPSVLFSFKATSGYGNNLYVDDINLTGVTSVHQIEGVTSLLVYPNPATDVLNITA
                     TLEKSLPMTISLTDISGRQLFQKNLGTVSVVDETVKTT"
     assembly_gap    13760..13859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13860..14399
                     /locus_tag="PKY09_08760"
     CDS             <13860..14399
                     /locus_tag="PKY09_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12987.1"
                     /translation="DASLNAYDVYKVPGTEHNFCWGILCYGVGVMSSAIPATINAGAT
                     EGSFRGDYLGHGYVGTTTVAYTVFDGNNPLDSVQFAINYHITPVGIKEVNRMNSISAP
                     SPNPARSITAFNYNIKSGSKAEVKIINMLGKTVKSIDVNTSTGNVVLSTADLPSGVYF
                     ISLNVEGKNSAVQKLVVNR"
     gene            14419..14931
                     /locus_tag="PKY09_08765"
     CDS             14419..14931
                     /locus_tag="PKY09_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB12988.1"
                     /translation="MNHFKKLVVAFSCVVSVSSAQVFESRPIPAVDVKDLNGKTVNTS
                     TLSNNGKPMVLDFWATWCKPCVNELTAISEHYEEWQKETGVKIVAISVDDARTMANVK
                     PFVNGKGWDYEVYVDPNGDLKRALNIGAVPYVFLVNGKGEIVSVKTGYAPGDEHKMYE
                     EIKALAKESN"
     gene            14952..16595
                     /locus_tag="PKY09_08770"
     CDS             14952..16595
                     /locus_tag="PKY09_08770"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNB12989.1"
                     /translation="MNKMIKLITGRILPVIFLGVPAITLAQNSPGEIHGSFQIDGQYY
                     IQDSTISAPEVAEKYLLNGYGDLVFTKGNFKAGVRYETYQNVLLGYDKRFNGSGIAHR
                     YAEYDNGELAITVGNFYEQFGSGLILRSYWEPTLGYDNALDGIRVKFKPYSGILIKGI
                     IGKQRYYFKTGEGIVRGVDAEVGINDLLKKWNDKKTRVTAGFGFVSKYQKDDDNVYIL
                     PENVGAYAGRLDVTRGKVTINAEYAYKYNDPSSLNNYSYKFGDAALLKATYSQKGLGI
                     LLAAKRIDNMNFRSERNANLNDLLINYLPALTKYHTYSLASIYPYATQPNGEFGFEAE
                     VNYTIKKGTKLGGDYGTNIIVNYSGANSLSTEPVAGKDLYKSDWGKFGKDVYYKDFSL
                     EITRKMSKNLKITLMYINQVYDKDKIQFQGSKQYGTIYDNIIVADFTFQINEKNAIRT
                     EWQTLQSEQDYKSWLAGLIEYSHSPNWFVAISDQYNYGNEKSDHRIHYFNCFVGYTKD
                     SHRITLGYGRQREGLFCVGGVCRQVPSSNGLTVSISTSF"
     gene            16616..17770
                     /locus_tag="PKY09_08775"
     CDS             16616..17770
                     /locus_tag="PKY09_08775"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNB12990.1"
                     /translation="MKNKFWLAIAALTVSTIVSCDKVDGPYKEENTTNIGSLPGTLND
                     IRVLNATTTSDDVDVLVAKFSLESASPRTIALQFTWHGAQSNVISEVATGNDAIFDND
                     SVYITAPAPFGTLTFKYSATISDTLTGQADFVVPYTPTNPVKKVLLEDYTGRKCGNCP
                     RAQRIIANTLEPLYHEQLVVATNHAGVYAVPDNPPSCFAEDFRNPNSDELNTFFGLSA
                     YPSSMINRVGYPTTHIKYYNSWQSTISQELSKPNDAFINIYSAYNEGSRTAQLTISTE
                     FLNNLGDDYKLAVFLIEDSIHGCQLDYDLTTPTQIDSAYIHRHVLRAALNSSFGENLI
                     NDPDLTTKYIRLYNYQIPDKYNVDQCYILAYVYQTNNYYVLQSELRKIKP"
     gene            17856..18299
                     /locus_tag="PKY09_08780"
     CDS             17856..18299
                     /locus_tag="PKY09_08780"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNB12991.1"
                     /translation="MKAVTDADSVISLEFDDEYSAYEVIEGTVAQQIVNELQEYFSGK
                     RKVFTLNLKPQGTVFQIQVWEKLLSVSYGKTISYNEQAIQSGNVKNIRATASANGKNP
                     IAIVIPCHRVIGTSGALTGYAGGLWRKERLLQLESKSRQLEPFSQ"
     gene            complement(18310..>20997)
                     /locus_tag="PKY09_08785"
     CDS             complement(18310..>20997)
                     /locus_tag="PKY09_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB12992.1"
                     /translation="YDKTGQRRRLQMFENAGNKHYNGCQWYPKMCVYDSHRAWNTDQH
                     LGREFYGEYGVYDVELTFANNFIVEATGVLVNEKEVLPDDLRKKLDIKNFKDRPASTQ
                     PSTIIEADGSTKTWKFHAENVHDFAFTADPSYRLSDAYWNGIRCVAIAREQHCYGWQS
                     AAEYTARIIEVFSRDFGMYDYPKIVTADANDGMEYPMITMDNGTDPGFRGLLCHEIGH
                     NWFYGMVGNNETYRPLLDEGFAQLLKSWGMEMLEGYHVQQREPTWKDQKRTEPLTYQM
                     MMAYGPYFNYVLNDKDGFINTHSDGFSSALGHGGGYGMVYFKTATMLYNLQYILGDSL
                     FLKAMQHYVAQWKFCHPYPEDFRNSITEYVKTDLSWFFDQWMETNKKLDYKICKYRKA
                     DEKDAFIVYFKRKGRMQSSIDFSVYSNCGTKYDYHIPNTWYVKQTDAMVLPKWEGWDK
                     LRNTYKAQIKIPCGISSIVIDTTDRLGDAYRLDNRLPRPFKLVFDKWQKPAYSDWKSY
                     VFYIRPDVWFNNYDGLKVGAHAEGNYMNTMHFFSADIWINTTVGQNSLSETADINKND
                     PASFRINYKTPLKFIAPGVNFWIGGGILDGLTFAKSGGDLTFLNNRIRLFTEYKLMYR
                     RNTNTLEYLLYPNEWQSASMNTSVTLGAEAQYRKNKWNGSVAIQNTSASLFSDYSYSK
                     LSLTNITNYETGKIDWRLRVFGQYGYGQNVPMESALYLAGANPEELMENAWTRSRAFV
                     PYSWLGYGDKTNHFQQGGGLNLRGYAGYLVPQNIDGEQRYFYRAISGASVSLEIDFER
                     LIPLRPSFTKQWLKADLYVFGDAGAIATNNSNKSIQFTNLKSDAGIGTAFTIKKFGRF
                     NAIKPLTIRCDFPFLLSSAPYADKNYVKLRYVIGIGRTF"
BASE COUNT         6755 a         3562 c         4029 g         6551 t
ORIGIN      
        1 gagattatta tagaatttca catggtgacg agtttactga tgctttcctg aaaccgggcg
       61 ataatcttta ttttacactt gacaccagaa tgtttgatga gtcggtaaaa tataaaggtg
      121 ttggtgaaaa tgaaaacaac tacttagctc aaaaaatact tttgtcagag aagctggagt
      181 tattggataa catggctttg attggtggtt atgatgaaaa aaagttttta gaatcagttg
      241 attcagctgc tacccttaaa aatgaattac taaaaggatt tgttttagag aaggaatttg
      301 ctgctgcaga aaaaaaagac attgaatttg cccaattaga taacatagca agttatgaaa
      361 tgtaccatag atatataaag cataatgaag attttaaagt gtctgaaagt tatcctgatg
      421 catttaaaaa ttttaatttt aatgatgagt catcagtaaa gaatgagctc tatttgaaat
      481 tattgtcaga ttttttttct aaacaagcat ataaacagat tcgggaagat tctacccgga
      541 attttttaat ggaattttca aatgcagttt tggaaagggt gcagagtact atagttagaa
      601 atggtttagg aaggttgatt gtcgaagatg gccttaagag tggaaatgat ttgaaagttt
      661 cttatgctgc tataaagaaa ttgatttctg acaaagatgt tcaagcaagc ttagatgaaa
      721 agtttgctat gttgagtaaa ttgcagaaag gcaatacttc acctgatttt tctcttcagg
      781 atattaaagg aaaaactttt tctttgagtg acttcaaagg taaggttgtc tatattgatg
      841 tttgggcaac ttggtgtggt ccatgtaaag ccgaaatgcc atttatgaaa aaaatacagg
      901 aagatttaaa aggaaaggat gttgctttta ttggaatttg tgcttgggat aagcaagaaa
      961 actggaagaa atacatagaa gaaaacaaga tgcagggaac acaactattt gcacctgata
     1021 gggcattgcc gtttctaaaa gaatatgata ttaaaggtat tccacgtttt atacttcttg
     1081 ataaagaagg caaaatagtt gaggctgatg cagcgcgccc atctgaccca cagttgaaag
     1141 cgaagataga gtcgttgctg taatacatgt aattttcaat cacttcaagc aaaactgttt
     1201 ttgctttcaa agtattctaa cttgcaaata ttctaacttt ccgacctgac aatgacatca
     1261 taatcatacc cggctctaat caatgctctt ttagcagcat caaatgcaaa agttgtagga
     1321 ttaggcggat gtagtggaac ttcaggtgtg tttgcaagtt gttgtaataa attctgagtc
     1381 tgagcatgtg tcagtaactc acatatatca taaagccctc cgggtttctc ttcttcaaaa
     1441 tcatgctttt cgagaatatg ttttataact ttaattaagt caggagtagg tggtggaacc
     1501 attaatgctg tcaatgcacc atgttccaat ctaaattgag gtaatatctc tttcatcttg
     1561 tttgcatcaa tagcttttgc tgccggaaac aaaatgtttt cctccatttt tatatgtcgc
     1621 aataaacctg agcgaaaagg catataaaat gtcatatcaa tttcaggaat tgtaattatg
     1681 gctttatcta atagcttttc taaacgatga tgatcggtag tgaaaaagtg atgtagtggt
     1741 ttgttcattt gatttcaaaa tgaattttaa aactgcaaat tacgggagat tcttttaaca
     1801 tttagtgaca gaatttcttt ggacaactat tcttattttt gcaacatata ggtatatgag
     1861 taaaatttct gaaaaagaag caacagaaaa gattaaacag ctgactgttg atcttaacca
     1921 tcataattat ttgtactacg taaaggccca acctgtcatc actgattatg agtttgatgt
     1981 catgcttcag gaattgattg cattggaaaa tcaatttcct gaattgcgga gttccgaatc
     2041 gccatcacaa cgtgtagggg gatatatctc taaagagttc ccaacggtta ctcataatta
     2101 tccaatgatg tcgttgggta acacttattc agaagaggaa ttgcgcgatt tcgatgaaag
     2161 gatcaggaga gtaattggta gcaattttga atatgtatgc gagttaaagt ttgatggtgt
     2221 agctattggc ctgacgtata gaaacggaaa actgcttagg gctgtaaccc ggggcgatgg
     2281 tgttcgtggt gatgatgtga cgaataatgt tcgaactatt cgatcaattc cgttatgtct
     2341 tatgggcaat aattttcctg atgagtttga aataagaggt gaaatcattc tcgataaaaa
     2401 aacttttgaa agaataaata atgaacgtgc agacattggc gagacacctt ttgccaaccc
     2461 gagaaattca gctgcaggca cacttaaact acaagacagc agtgaagttg caagacgtaa
     2521 acttgattgt tttctctatg cactatatgg tgattttgat ttttcaacac attatgacgg
     2581 acttaaaatg gctgccgaat ggggatttaa aatttcatca cattacagac tttgcgattc
     2641 gattgaaaac gtatttcaat ttattgaaac atggagacaa aaaagggatt cactaccatt
     2701 tgatattgat ggcatagtta ttaaagtaaa caatttccgt cagcaagagg aactaggctt
     2761 tacagcgaag tcgcccaaat gggctattgc ttacaaatac aaagcagaat cggctacaac
     2821 tattctcaat gaagtagttt atcaggttgg ccgtacagga gcaataacac cggttgccaa
     2881 tttaaaacct gttttattgg cagggacaac tgtaaaacgt gcttcattgc ataatgccga
     2941 tcagattcag aaacttgact tacatctgca cgacactgtt tttgtagaga aaggtggaga
     3001 aattataccc aaaattaccg gagtggacct atcaatgcgt aaagccgggg caatagctgt
     3061 caaatacatt acacattgcc cggattgtgg agcagagctt attcggcagg aaggcgaggc
     3121 caatcactat tgcccgaatg aaaatggttg tccaccacaa ataaaaggta ggttggtgca
     3181 ttttacttca cgaaaagcta tgaatattga tggacttggt gtagaaacca ttgaccaatt
     3241 agttgaagcc ggactactta aaaatgttgc cgatatctat caccttaatt ccgatgactt
     3301 actgcaactt gaaagaatgg gagaaaagac tgttggtaat ttgttcgaag caattgaaaa
     3361 atccaaacaa gttccttttg aacgtgtgct ttttgctata ggcattagat ttgtaggtga
     3421 tactacagca aaaaagttag cacgtcattt tgggtcttat cataaaatca gccaggcaac
     3481 acttgaagaa ttaatgctaa caccggaagt aggcgataaa attgcagcaa gtatttttga
     3541 ctttgttcgt cagccggcta acattaatat ggtagcgcaa cttcaggagt caggactgtt
     3601 gtttgaactt gatgcttctt cagtgcgtga cgcaggaaca aaacttaaag gattgagttt
     3661 tgtgattaca ggagtatttc agaaatttga gcgcgataaa ttaaaagaaa caatagagat
     3721 taatggaggt aaggtttcag gctctctcag ttcaaagaca agttatctga ttgcaggtag
     3781 tgactgtggt ccggctaagc ttgaaaaagc agagaaactt aaaatcaata ttatctcaga
     3841 agacgatttc ttaaaaatgc ttcaatgaaa ataggtaagg caataaaaca aaaggctgcg
     3901 agctatttat tttaccgtga gttgcagcgt cagcatcatc aaccacgcac tactagtttt
     3961 agtaatgccc gtactattgg tattttgtat gatgccacta ttgagcgtaa ttatgaaatt
     4021 gtaaaagcct atgtaaaaca tctgcgtgac gattttaaga aagatgttct tgcacttgga
     4081 tattatgatg ccaatgagct tccgcctatg cgattttcaa aacttggact tgattttttt
     4141 accaagaaaa atctcaattg gcatttcaag ccttcacatc ctatgatttc caagtttatc
     4201 aatctcgatt ttgattatct gatttgcctt aatgtagatc gctgcatgcc attaaggtat
     4261 atcagcgctg caacaaaggc aggttttaaa ataggacgat atgaaaaggg gtacgcaggg
     4321 ctttacgatt ttatgattag tgttaatggt caggttacac ttcctcagtt tatagaccat
     4381 gtaaataatt atctcaaaca tattggacac gataaatgaa taaattctca gggacaggtg
     4441 ttgcattggt aacacctttt ttaaaaagtg gaaaaacaga ctttgatgca cttcgcaagg
     4501 tagtcaatca tgtaattaaa ggtggtgttg attatttagt cgtaatgggt actaccggtg
     4561 agtcggtgac cctttctaaa gaagaaaaaa aggatatttt aaaatttatt ggagaggtta
     4621 ataaaaaaag aacacatata gtttttgggg taggaggaaa caacacactt gaaactgcgc
     4681 gtgaactaga acacatagac gaccaaatgg cagatgccat tctttcggtt tcaccgtatt
     4741 ataataaacc aaatcagtcc ggcatagtgc agcattacaa aacactttcc ggtgcatcac
     4801 agttacccat tattctttat aatgtaccgg gcagaacagg cagcaatcta ttaccacaaa
     4861 caactcttga aattgccgca agttgtaaaa atatcattgg tataaaagaa gcttccggta
     4921 acatggaaca gattatgtat ctgatacatc ataggaacaa aggcttttta gttatttccg
     4981 gtgatgatgc cattactatg ccgttaattg cttcaggtgc cgatggtgta atttctgtta
     5041 ttgctaatgc ctatccaaaa caattcagca cgatggtacg tcttgcattg caaggtaaga
     5101 tggtagagtc acgtaaactc cattatcaac ttcttgattt aataccctta ttatttgctg
     5161 aaggtagtcc atcagggatt aagtatgttt tatcacaaat gggtatttgt cagaactatt
     5221 tcagattgcc tgttgcacca gtcagtaaaa cattggcaca aaaaattgat aaagtcatta
     5281 aagagatctg atttaaataa tgcgctactt gttttatatg ggtcatccgg cccattttca
     5341 tttgtttaaa aatacagttg ttgcattaca gcaagcaggt catgaagtgc agattctcat
     5401 taagaaaaaa gatattcttg aagatttact ccaacatgct ggtatgcaat acatcaacat
     5461 caatcctgag ggcaggaggg atgataagtt ttctattgca ttaaaattac tcaaacgtga
     5521 ttttcagttt tttaaaattt gtctgcgatt taagccgcag attatgattg gtacatctgc
     5581 tgaaattgca catgttggaa aattacttgg catcagaagc attgtggtta atgaagatga
     5641 tgcagaagtt gttccatggt ttgcaaagtt agcttaccca tgggcgacaa atattcttgc
     5701 gcctcattgt tgcagtgtag gcaaatggga acataaaaaa atcggctata aaggttatca
     5761 tgaattagcg tatctgcacc ctgcacaatt ttcaccccaa aaggaaaaca taaaaaattt
     5821 gggcgatgag ccttttttta ttcttcgttt tgcaaaactt acagcacatc atgacgaagg
     5881 ccgtaaggga ataaacactt taattgcaga gaaattaatc aatatacttt ctaaacatgg
     5941 ccggatttat atcacttcag agcgggagtt agaacctcag tttgagcagt ttcgtattgc
     6001 cataaaccct ttacatattc atgatgcatt atactttgca cagatgtaca ttggcgatag
     6061 tcagacaatg gctgctgaag ctgctgtttt aggaacacct gccattaggt ttaatgactt
     6121 tgttggtcag ataagttact tggaagagct tgaaaaggag tttgcattaa cgtttggctt
     6181 taaaactaac gatgcagatg ctatgctgac gcatgtagaa tctttattaa atcaacagga
     6241 tttaaaacag cattggcaag aaaagcgaaa tcagatgctt aaccaatgta ttgatttaaa
     6301 cctatttatg attaaaatgc taacgcagca gtaggttaaa agatccgaaa attttttaag
     6361 ttgatgtttg aaatgtttta aagaaaacac ttgcgcattc cgaaattcgt gtttatctta
     6421 gcaaaccaaa atcgaattat tttaaacttt tttcaacttt ttttaagtat tgatctttaa
     6481 aaccgataac actgaagcaa aacttttgca gttagaggag tttgctgaat tggatgtagt
     6541 attggctgct gaagccaaaa ataatgtcca aaatgagcgg tttgtggtta atcttattga
     6601 aaagtatgtt gatacttcac gaagtgattg tttcacattt gctgcaaaca accatgatga
     6661 tgccaatcaa ttagctgacg atacataccg tgctattatt cattttaaaa gagtaaataa
     6721 ttttcatttt atcaataagc agtttgaagc aataaatacc aagttgccca agggaggtat
     6781 ttttgtcagt aaggttgaga ctaacgacct gcgtaagcaa cgcctgatgg aaaagtatcc
     6841 accatatttt aataaggttt attatttctt tgattttatc tttaaacgcg tatttccgaa
     6901 actgcctgtt acacaacaaa tttattatgc catcacaaaa gggcataatc gtgtgttgtc
     6961 taagacagaa atgctcggaa gactctattc ctgtgggttt aaagttttag aagaacgtta
     7021 cataggtaat agactttata tcgtttcaga aaaatttcgt gaacctgcat atgatgaaga
     7081 ccctagttat ggtataatct atccaatgaa acgtgttggt aaaggcggta aaattattag
     7141 cgtttacaaa ttcaggacta tgcatgcgta tgctgaatac cttcaggagt acatttatga
     7201 aaaaaacaac ctcgaagaag gtggtaagtt taaagatgat ttcagagtaa gccgtgtggg
     7261 tcgcatgctt cgcaaatatt ggattgatga gttaccaatg attttgaatg tgcttaaagg
     7321 tgatttaaaa attgttggtg ttcgtccttt gagcagacat tatctgagtc tttattcaaa
     7381 agagctgcag gagatgcgta aaaaatttaa acctggactt atcccacctt actatgttga
     7441 tcttcctaaa acaatggaag aaattatgga ttcggaaatg cgctatttaa aggcctatca
     7501 aaaaaaccca ttcctaacag atgtacgtta ttttttaatg gtgttttata ccatcctttt
     7561 caagcgtgcc agaagtaaat agacagccgg ttttccttta gttatttttt tcatctttgc
     7621 gcaatcataa ttttaaattt catgaaagta atccgtctga tttttatttt gattctgatt
     7681 gttgcttttc aattaaattc atctgcgcag tatgtgcctc aatcagtatt caataaacca
     7741 ctttatgatt ttatggatga cctggctaat ctgcacatca ttacacttaa tgatgcaata
     7801 aaaccatact caagaaaaca gattgcagaa gccttacaag cagccgaatc acaaaaggaa
     7861 aaattaaaca caacacaaca aaagcagctg gctttctatt taaaagattt taataaagaa
     7921 ttgagtacaa ctaaaggatt taaaaaacgt ttggatttat tttattttaa agactcgcta
     7981 tttaatatta cagttaatcc tgttggtgga ttcagttatt ttaagaatga aaacgacaat
     8041 atgtatcgtc aatatgcagg tggtgagttt tgggcaacct taggaaaagg acttagtatc
     8101 tttggaagtt atcgcgatta tactgacagt agagctgttg cagatgataa atatctgaat
     8161 gacttaccgg gatataatta taaatataaa ggtgctgttg gctcaccaaa acgcggaggt
     8221 agctttgatg aaacccgtgg aggcattgcc tatgcatgga aatggggaca tatcggaata
     8281 gtaaaagata atcttgaatg gggcagcaat aatcatggtg caaatattat ttcaacaaaa
     8341 gcaccatctt ttgcccgatt agaacttaaa cttaatccaa agaaatggtt tgagttcaac
     8401 tattttcacg gctggcttta ttctgaaatt aaggatgatt ctgcagcata tttttcaggc
     8461 aacccaccta atcacagaca agtatatttt tcaaaatatt ttgcagccaa catgttcact
     8521 gtaaaacctt ttaaacaatt cgatttcagt tttggcaatt caattgttta tagcgatcaa
     8581 ttggaagtag ccatgtttat tccgtttatg ttttttaaat cacttgacca tagtaaaaca
     8641 ggtcagggta gtaactattc agggcaaaac tctcaattgt tttttaacat cagttcgcac
     8701 aacataaaat atacacatct ttatatttca ttatttgttg atgaaattgc gttcggtaga
     8761 atgatggata aagacgagca ttcaaatttt tacagcctaa aagcaggtac tgctgtgatg
     8821 ctgccatttt atacttcggc tactattgtt gcagaatata ccagaacaaa tcctgttact
     8881 tacaggcatt ttgttaatac cacaacctac gagtcaaaca gatttaatat gggacattat
     8941 ctccgcgaca atgctcagga aatttatctt ggtgcacgtt gtacgccaat atcaggttta
     9001 tatatttcag caggatacac cacggcagcc gttggcccta tttatcctta tacaggcaaa
     9061 gacaagtcgg gattgggttt gccattttta aaaacaaaag aatatgagtt tacctctctt
     9121 gatttgaata taaagtacga gttgtttaat gacatttttt tatctgcagg tgttaaaatt
     9181 gatgataata aaggtgcatt ggcaaacgtt tatactgcac cattttatta tggtaaaaat
     9241 ggtaaaacaa caactctttt cggtggattt aatataggat tctaaaaatg aactttgata
     9301 atttaacaac agcactcctg atcattgctc caacatttat tgttgccggt ggcatgtata
     9361 tgctcataaa aaatatgatg gaacgcgatt acagacttaa acttcttgaa gcaagaaggt
     9421 taatacaaaa agatatgctg ccattaaaac ttcaggcggt tgaaagaatg gttgtttttc
     9481 tggaacgcat tcaacctgaa aatatcttat tcagaatttt gcagcccggc atgaatgtgc
     9541 gcgatcttca ggtagatttg cttgctgcca ttcgacagga gtacgaacat aatgttagtc
     9601 agcaagtgta tgtttctgta caaagctggt cattaattac aaaagcaaaa gacgacataa
     9661 ttgcaactgt aaaccttgca gcagattctt tagacaagac tgaaaaggca attgtgctga
     9721 gtcaaattat tttgcagcgc tcgtcagcag gtaataattt tgccggagca gcaattctat
     9781 cactcaagag cgaagtagat agttttttgt aaaactcttt tttatacctt agcccgagaa
     9841 gggttatgtc gttttccaca agaatcattt ttaagactgc tgtgaagctt caacttcaaa
     9901 atttgctgaa gttgattcct gtttcaccaa atggagtgac aatactttgt ttccatcgaa
     9961 tttcatcaca atacgattat ttctggcaac caatttatcc ggaaaccttt ggtttgatgc
    10021 tggagtcttt ggttaaggaa tatcagatta ttccatttag tcagatagat aatacatttg
    10081 ataaaaacgg taagccacct ttggtattga gttttgatga tggctataaa gattttttgg
    10141 aagaagcaat acctttatta cagttttatg aagtgccaag caatcataac attgtgatag
    10201 attgtgccga tggcaaaaat tatatctgga cacaaaaact gaatcattta tttaacctgt
    10261 tacgcaatag cagtcgcttt tcaatacatg tcgcattaga caaacacttt atacttgata
    10321 ttactcaacc ttgcaaaaaa tggttcgttc attattatag aactttattt tatctgttta
    10381 agcttgatgc gaaagcaaga gcagaatgta tttcagtttt agagcagtat ctgaatattg
    10441 cagaaaatga ttatcccatt aaaatgatga attgggatga tattaagttt ttatctttaa
    10501 ataatgttga aattggttgt catactacct cgcatgttag tctgccttct gttgagaatg
    10561 atgaagaatt ttttcttgaa ttggattacg ctaagaaaag gatagaagag gagcttaaat
    10621 gtgaatgtcc tgtaattgcc ttgccaaatg gtttgacaaa taatttggtt aatcagaaaa
    10681 tagctgatgc aggctttaag aaaattcttt gtctggcagg ttcaggcagt aatgcaagta
    10741 aatctaccga taaagtaaaa ttttataaca gattaaatat gattcaggag ccggttagat
    10801 atatgaatgt tagaattaaa ttttatgaaa aagggttttt gagatgagtg acttacaatt
    10861 gcgtcaattg cagaataacg agcttaattt attaccaaag ttaatgcaag attgctttaa
    10921 catgaaagtt gacgaagatt attttaaatg gaagtactta aataatcctg ccggaaattt
    10981 tattggtttt ggtgcattta gtaataacag tttagtaggt tttaaaggtg tgttccccgg
    11041 tttgtataat gttagaggga ctcagacaat tatttatcaa tccggagatg ctatgacgca
    11101 tccggatttt agaagacaaa gattgtttga gaagttggca acatcctgtt ttgaaaaaat
    11161 aaaagaaaaa catcaactta ttgtgattgg ttttggtggc acaaaatcta cccctggctt
    11221 aataaaaata gggtggacac accttgctga tgtgaagttt ttctttattc ctaagttgaa
    11281 atgtagatgg aatagcttgt tttcatttag attaaagcat tatcagtgca atgaaacctc
    11341 tgatcttaat gagatagtaa cactatataa taaatatggc gtaaaaaaag acattactcc
    11401 aatttatagt aatgaatttt taacctggag gctgtccaat ccacgcttta agtaccattg
    11461 cgtagtttgt gaaaattcaa acgagcggtt aggctttttg atttattatc aggatggcga
    11521 taaaatcttt atactcgatt attgtggcaa aaacgataaa acattaagtg cattatttaa
    11581 ttatgtgagc aaagtttgta tagaaaaaaa actaaagggt ttgataacaa ttgcaattcc
    11641 gaaatcttca gaaggtaaac aattaaagcg ttgcggattt ttgcaaaatc catttcaagc
    11701 cggaccaatg agtgccaaaa tcccatttat agtactgact gaaccttcat taaaagaaca
    11761 ctttcttcat caaaatcaat ggcttgtaat tccaatttgt catgatgcac tatgatgact
    11821 gtgacttaaa atactcactt gagcgtttat tttaccaata tgttaaaaat ggcagttttt
    11881 agacattggt ttactggata gtatgtactt ttatgcaata attttaaaat caaatattta
    11941 taataatgaa aaaaatctct accattttaa tggcagcgtg tttagtagta ggcctggcaa
    12001 atggccaatc acagcgctat gttgttgttg aagaattcac cggtgaaacg tgtggcccat
    12061 gtgcatcaaa caatccgggc ttcaacgcca agttgaatgc aaactcctac actttgccaa
    12121 tcaaatatca gaataacatt ccttctaacg gacctaattt ttatgcttat aacactactg
    12181 atataggtaa cagaacatca tattatgcaa acaattattc acctcatgca tttttagatg
    12241 gtaatgtttg gaatggcaat gcgggctctt ttccggttgc aacacttaat tcgagggtgg
    12301 cagcaacctc accatttaca atagatgtta cacacagttt ttcgcccacc cacgatatca
    12361 tttatgtaca tgctgttatt cgtgctacac aagctgtgac aggattaagt agtttaaaag
    12421 cacgtatcgc aattacagaa aaagacaact atggttatac ctctccaaac ggagaatcac
    12481 actattcaga tgtaatgcgt aaaatgctac ctaatggcac tggaacagta ttgcctgcaa
    12541 cctgggctgt aggtgattca gtagttttag atgagcagtg gacaataaac gttccatcca
    12601 atatacctac tgtatttcta ccttattggc caatgcttcg tgctgtagtt tttgttcaga
    12661 acgatgctaa caaagaaata ttacaaggtg gcagaagcac agttgccgct gtagttccat
    12721 tagctgcaat taccaatctt tctacaatgt tttcctgtac cagttcatta gacccagcag
    12781 taactttaac caactatgga ggaggtggca accttacatc tgctgttatt gaatttggaa
    12841 tcactggtca ggcaatgcag acttataatt ggtccggtac attagcagcc ggtgcatcaa
    12901 cgataataac attacctaat atgactttac ccggtggtgg catgcaaaac ttttctgcta
    12961 aattgacaag tattaatgga aacccggatt attataacat caacctgaac tttagtgcac
    13021 ctgttgcaca agcagcagca ccaactacat cttttagtca ggatttttat tcaacagtgt
    13081 ttcctccggt gaattggatt ttgaataatg ttgataatgg ctttacatgg actagaagtg
    13141 cagtaggtgg ttttcaaatg aacccaggtg gttctgcaag aatgaatttt tataattctg
    13201 caaatggcca ggtggacgaa cttttaccat atacaccagt tgattttacc aatgctgtaa
    13261 accctcaatt gactttcagt gtggcatatt gccaatattc aaatgaaaac gatcgtttag
    13321 atgtgcgtgt ttctaccgat tgtggcgcaa cttggtcatc tgtatataat aaagcaggtg
    13381 cagcactttc aactactgct gcacaaacta cttcctttac gccatcatca gcatcacaat
    13441 ggcgcgcaga aacagtttca cttgctaatt atgtaggaca accttctgta ttattctctt
    13501 tcaaagcaac atcaggttat ggtaacaatc tttatgttga tgatattaat ctgacaggtg
    13561 taacctctgt tcatcagatt gaaggcgtaa cttcacttct ggtttatcca aatcctgcaa
    13621 ccgatgtact gaatatcaca gctactttag aaaaatcgct tccaatgaca atttccttaa
    13681 ccgatatttc aggaagacag ttgtttcaga aaaatctcgg cactgtttct gtagtagatg
    13741 aaactgtaaa aactactgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    13861 atgccagcct gaatgcatac gatgtttaca aggttccggg tacagaacat aatttctgtt
    13921 ggggtatttt atgctatggg gttggcgtta tgtcatctgc aatacctgct actattaacg
    13981 ctggtgcaac tgaaggatca ttcagaggag attaccttgg acatggttat gtaggaacta
    14041 caactgtagc ttacacagtg tttgatggta acaatccatt agattcggtt cagtttgcca
    14101 ttaactatca tattacgcct gtaggtataa aagaagtaaa taggatgaac agcataagtg
    14161 cgccatcacc aaatcctgct cgttcgatta cagcatttaa ttataatatt aaatcaggaa
    14221 gcaaagcaga agttaaaatc atcaatatgc tgggtaaaac agttaagagt attgatgtaa
    14281 atacatcaac aggaaatgtt gtactttcaa cagccgattt gccttccgga gtttatttta
    14341 tttctcttaa tgttgaaggg aaaaattcag ccgtacaaaa gttagttgtt aacagataag
    14401 aatcaattta aagagtaaat gaatcatttt aagaagttag tagtagcctt ttcttgtgta
    14461 gtatcagttt cctctgcaca ggtgtttgaa tcacgtccta taccggctgt tgatgtaaaa
    14521 gacctgaatg gtaaaactgt aaacacatca acattaagta acaatggcaa gccaatggtg
    14581 cttgattttt gggctacttg gtgcaagcct tgtgtgaatg aacttacagc gattagcgag
    14641 cattacgaag aatggcagaa agaaaccggt gttaagattg tagccattag tgtggatgat
    14701 gcacgtacta tggcaaatgt taaacctttt gttaatggca aaggctggga ctatgaggta
    14761 tatgttgatc ccaatggtga tctgaaaaga gccctcaata tcggagcagt accttatgtt
    14821 ttcttagtaa acggcaaagg ggaaattgtt tcagttaaaa caggttatgc acctggcgat
    14881 gaacataaaa tgtacgaaga aattaaggca cttgccaaag aatcaaatta ataaataact
    14941 atttgctttt catgaataaa atgataaaac ttataaccgg cagaatattg ccggttattt
    15001 ttttaggtgt tcctgcaata actcttgcac aaaactctcc gggtgaaata catggaagtt
    15061 ttcagattga cggacaatat tacattcagg attcaactat aagtgcacct gaagttgccg
    15121 aaaaatattt gctcaatggt tatggagatt tggtttttac caaaggtaat ttcaaagcag
    15181 gtgttcgtta cgaaacctat cagaatgtat tgcttggcta cgacaagaga tttaacggca
    15241 gtggcattgc acatcgctat gccgaatatg ataatggcga attagctatt actgtcggaa
    15301 atttttatga gcagtttggt agtggactga ttttacgatc ctattgggag ccaacactag
    15361 gttatgataa tgcattggat ggtattcgtg taaaatttaa accttacagt gggattttga
    15421 ttaaaggaat tattggtaaa caacgttatt attttaaaac cggagaagga attgtaagag
    15481 gagtagatgc tgaagtaggt ataaatgatt tattaaaaaa atggaatgat aagaagacca
    15541 gagttactgc aggctttgga tttgtgagca agtatcagaa agatgatgac aatgtatata
    15601 tcttgccgga gaatgttgga gcctatgccg gacgacttga tgttaccaga ggaaaagtaa
    15661 ccatcaacgc agagtatgcc tataaataca acgacccttc atcattaaat aattacagct
    15721 ataagtttgg tgatgcagcg cttttaaaag caacttattc tcagaaaggt ttgggaatat
    15781 tacttgccgc caaaagaatt gataatatga acttccgtag tgaacgtaat gctaatctaa
    15841 atgacttgtt gattaattat cttcctgcat tgacaaaata tcacacctat tcgttagcct
    15901 ctatttatcc ttatgcaaca caacctaatg gtgaatttgg ttttgaagct gaagtgaact
    15961 atacaattaa aaaaggaaca aaattaggtg gcgattatgg aacaaatatt attgtaaact
    16021 attcaggagc taattcatta agtaccgaac ctgttgccgg taaagacctt tataaaagtg
    16081 attggggaaa attcggcaaa gatgtttact acaaagattt tagcttggaa attacccgta
    16141 agatgtctaa aaatcttaaa attacattga tgtatattaa tcaggtttat gataaagata
    16201 aaattcaatt tcaggggtca aagcaatatg gaactattta tgacaacata atagtagctg
    16261 attttacctt tcagataaat gaaaaaaatg ctatcagaac agaatggcaa acattacaat
    16321 cagaacaaga ttataagtca tggcttgccg gactgattga atattctcat tcaccaaatt
    16381 ggtttgtagc aatatcagat caatataatt atggcaatga aaaatctgac cacagaatcc
    16441 attactttaa ttgttttgtg ggatatacca aagattcgca ccgcataact ttgggctatg
    16501 gaagacagcg tgagggtttg ttctgtgttg gtggtgtgtg caggcaggtt ccatcaagca
    16561 atggactcac agtgtcaatt agtacaagtt tctaaaaaat taaaagttca cgaaaatgaa
    16621 aaataaattt tggttagcaa ttgcagcttt aacggtatca acaattgttt catgcgataa
    16681 agttgatggg ccctataaag aagaaaacac aacaaacatt ggctcactcc caggcacact
    16741 caatgacatc agagtgctca atgcaactac aacatctgac gatgtagatg tgcttgtagc
    16801 aaagttttca cttgagtcgg catcacctag gactattgca ttgcagttta cctggcatgg
    16861 agcacagtct aacgtgattt cagaagttgc aacaggtaat gatgccatct ttgataatga
    16921 ttctgtttat ataacagcac ctgcaccatt tggcacactg acttttaaat attcagccac
    16981 cataagtgat acactaacag gacaggctga tttcgttgtg ccttatacac caacaaatcc
    17041 ggtaaaaaaa gtgttgcttg aagactatac aggacgtaag tgtggaaact gtccgcgtgc
    17101 acagcgtatc attgccaata cacttgaacc attgtatcac gagcagttgg tggttgctac
    17161 caaccatgct ggcgtttacg cagttccaga caatcctcca agttgttttg cagaagactt
    17221 cagaaatcca aattctgatg agttgaatac gttttttggg ttgtcagcct atccttccag
    17281 tatgattaac agagtagggt atccgacaac acatattaaa tattacaaca gttggcaatc
    17341 cacaatttcg caggagttaa gtaagccaaa tgatgctttt attaatatct attctgcata
    17401 caatgaaggg agcagaactg cgcagcttac catcagcact gaatttctaa acaatctggg
    17461 tgatgattat aaattagctg tttttctaat agaagacagt attcatggat gtcagcttga
    17521 ttatgattta acaacaccta cacaaattga ttcagcatat attcaccgac acgtgctaag
    17581 agcagcatta aatagttctt tcggtgagaa ccttattaat gatcccgatc tgacaacaaa
    17641 atatatcaga ctttacaact atcagattcc tgataagtat aatgttgatc agtgctatat
    17701 tctggcttat gtttatcaga ccaacaatta ttatgtgttg cagtctgaac tcagaaagat
    17761 aaagccataa accggcagat acttttaggt taaagttttt tgttttgcat caatcttcta
    17821 atcatacttc gtatatccaa acaccgttgg gttttatgaa agcagttact gatgcagata
    17881 gtgtgatttc actagagttt gatgacgagt attccgctta tgaggttatt gaagggactg
    17941 tggcacaaca gatagttaat gagctacaag agtatttttc cggtaaacgt aaagtgttca
    18001 ccttaaatct aaaaccacag ggaactgtat tccaaattca ggtatgggag aagctcctgt
    18061 ctgtatccta cggaaaaacc atttcttata acgaacaagc aattcaatca gggaatgtga
    18121 aaaatataag agctacagca tcggccaatg gaaaaaaccc gatagcaatt gtcattccat
    18181 gtcatagagt aatcggaact tcaggtgcat taacaggtta tgcaggcggg ttgtggcgaa
    18241 aggaaaggct actgcagctt gaatcaaaaa gccggcaatt ggaacctttc agtcaatagc
    18301 gtggtgttat cagaaagtgc gacctatgcc gattacataa cgaagtttga cataattttt
    18361 gtcagcataa ggtgcactgc ttaacaggaa tgggaaatca catcgtatag taagcggctt
    18421 tattgcatta aagcgaccaa attttttaat ggtaaatgct gtaccaatgc ctgcatcact
    18481 tttcagattg gtgaattgaa ttgacttatt ggaattgttt gttgcaatag cacctgcatc
    18541 gccaaataca taaaggtctg cttttagcca ttgcttggtg aatgatgggc gcaaaggaat
    18601 caatcgttca aagtctatct ctaaactaac cgaagcacca ctgatggcac gataaaaata
    18661 tctttgttca ccatcaatat tctgtggcac aagataacct gcatagcctc ttaaattgag
    18721 tcccccacct tgttgaaaat gatttgtctt atcaccgtaa cctaaccaac tgtagggaac
    18781 aaaagcgcgt gatcgtgtcc acgcattttc catcaactcc tccggattag cacctgcaag
    18841 atataatgca ctttccatag gaacattttg tccatagcca tattgtccga aaactcttag
    18901 tcgccagtca atttttcctg tttcgtaatt tgtgatattg gtcaatgaaa gtttagaata
    18961 ggagtaatca ctaaataatg atgctgatgt gttttgtatg gcaacagaac cattccattt
    19021 attttttctg tattgtgcct ctgcacccaa ggttacagat gtattcattg atgccgactg
    19081 ccattcattc gggtacaata aatattcaag tgtgttggta tttcttctat acatcaattt
    19141 gtattcagta aacagtctga tacgattgtt tagaaaagtc agatcacctc ctgactttgc
    19201 aaatgttaaa ccatcaagta ttccgccacc aatccaaaaa ttaactcccg gtgcaataaa
    19261 ttttaatggt gttttgtaat ttattctgaa tgatgcaggg tcatttttat tgatgtcggc
    19321 agtttcactc aggctatttt gccccacagt tgtattaatc caaatatcag cagaaaaaaa
    19381 gtgcatggta ttcatgtaat taccttctgc atgtgcacct acttttaatc cgtcataatt
    19441 gttgaaccaa acatcaggac gaatgtaaaa cacatagctt ttccaatcag agtaggcagg
    19501 tttctgccat ttgtcaaata caagtttaaa aggtcgaggc aagcggttgt ccaatctata
    19561 agcatcacct aaacgatctg ttgtgtcaat tacaatgctg ctaataccac atggaatttt
    19621 tatttgtgcc ttataggtat ttctcagttt atcccaacct tcccatttag gtagcaccat
    19681 tgcatcggtt tgttttacat accaggtatt tggaatatga taatcgtact ttgtaccaca
    19741 gtttgaatat acagaaaaat caattgaaga ttgcatgcgc cctttgcgtt taaagtaaac
    19801 aatgaaagca tctttttcat cagccttacg atacttgcag attttataat ctaacttttt
    19861 attggtttcc atccactgat caaaaaacca gcttaaatct gttttaacat attccgtaat
    19921 actgtttctg aaatcttcgg gatatggatg acaaaatttc cattgtgcaa catagtgttg
    19981 cattgctttt aaaaacaaac tgtcgcctaa aatatattgg aggttataaa gcatggttgc
    20041 tgtcttaaaa tacaccatac catagccgcc accatgacct aacgcactgc tgaaaccatc
    20101 actgtgtgta ttgataaatc catctttgtc attaagaaca taattgaaat atggtccata
    20161 ggccatcatc atctgataag tcaaaggctc ggttcttttt tgatctttcc atgtaggttc
    20221 acgttgctgt acgtgataac cttccagcat ttccattccc catgatttca gtaactgcgc
    20281 aaatccctcg tcaagtaatg gtctgtaagt ttcattatta cctaccatac cataaaacca
    20341 gttatgtcct atttcatggc ataaaagtcc tctgaatcca gggtcggtac cattgtccat
    20401 agttatcatt ggatattcca ttccgtcatt ggcatcggca gtgacaattt tcggatagtc
    20461 atacatccca aaatcgcggg aaaacacttc gataattctt gctgtatatt cagcagcact
    20521 ttgccatccg tagcaatgtt gttcgcgagc gatagctaca caacgaatac cattccagta
    20581 tgcatcagaa agacgatagg aagggtcggc agtgaaagca aaatcatgca cgttttctgc
    20641 atgaaacttc caggtttttg tacttccgtc tgcttcaatt attgttgagg gttgtgttga
    20701 agcaggtctg tctttaaaat ttttaatatc aagtttcttg cgcaaatcat ccggtaaaac
    20761 ttctttctca ttaactaaca cacctgttgc ttctacaata aagttgtttg caaaggttaa
    20821 ctccacatcg tacacaccat actctccata aaattctctt cccagatgct gatcggtatt
    20881 ccatgcgcga tgcgaatcat aaacacacat tttagggtac cattgacatc cgttgtaatg
    20941 tttgttgcct gcattttcaa acatctgtaa acgtctgcgc tgacctgttt tatcgta
//
LOCUS       DAOQNP010000058        20568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_889_length_20568_cov_8.060352, whole genome shotgun sequence.
ACCESSION   DAOQNP010000058 DAOQNP010000000
VERSION     DAOQNP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20568
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_889_length_20568_cov_8.060352"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKY09_08790"
     CDS             <1..330
                     /locus_tag="PKY09_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB12993.1"
                     /translation="LPNNPDYELGALQGSGCDTLTALKQDPEIAKNNLFVYPNPAKDF
                     VTIAFSKAVRTTCNLRLFNVLNKEVYRQNFVQQKVTVPIANLAKGIYIVEVSNNGFKE
                     YAKFVKE"
     gene            535..5928
                     /locus_tag="PKY09_08795"
     CDS             535..5928
                     /locus_tag="PKY09_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNB12994.1"
                     /translation="MSTLKYHFWTLLCIVLLSACKNQNTVSVANKNFEDVVEQQQNLI
                     FTFDKDLVDDSLLNRWDSTQFVAFTPAVKGLFKWNTKRELMFSPETSFGPATFYKAVV
                     TDKILFENRLLSLNVPAAYDFHTPLTQLEGLHAHWALSTQQNSIVLLAKLDFNYPVPS
                     AQMKDLAQIKINDKVYPFTVVTNDASTEVVLEINNMQPADFKKVNVSVSINKGLACNG
                     SNYKTESVLIKDADVPLPNRLTVMNASGEYDNTQPVIHVYTNQAVDMNQIAMYVTVQP
                     RVDFTTEAANDGFLVKGAFDPGQSYQLTVKKDLKGLIGGMLEQDFIATVPFGEMQPSI
                     SFTNAAGMYLSSKGNRNIGIQLVNVPKVRVSVYKVYENNILNFMRQNSYSNYYEESDD
                     GSYGGNQYNIYGIENYGDLVMQRNYSAKSLPRGNGINLLNLSLDEINAYKGIYVVDVA
                     STDDYWLHDTKVIALSDIGMIAKYSENELTVFTNYIKSALPAEGVNVSCISTNNQVLF
                     NGTTGHDGKITFSDIGSKAKNFRVGMISARQGKDFNMILLSESKVSTSRYDVGGYKDN
                     PAGVMAYIYGDRDMYRPGETVYLNTIVRNEKWMPVANVPVKLKMTMPNGHEFKTLRKS
                     LNSQGAVAADFLLPQAAVTGNYNIDIFSANDVLIGSRTIQVEEFMPDRIDVKVKTNKN
                     NFQPADTIIAAIAATNFFGPPAAMRKFEAEFNLVRKSFLSKEFSGYNFTVKTINELPV
                     DKKVEQGQTNEKGEATAKFLIPDSCKNSGLLQGRIYTTVFDETGRPVNRLNTFDVNTQ
                     RNYFGIKLNDYYANIGTNMVINLAACNSNGSGAAATAYVEIINYDWYNVIEKSDNGQY
                     RYVSQKREVTMLSREINIAAGGSNLNFIPANSGEYEVRISAVKGGNYVASNFWAYGYG
                     YTSNTSFNINTEGQVIIKADKDTFTPGEKANLLFKAPFAGKLLVTVESNRLLKHYYIN
                     TDKRSAMLSIPVDESFLPNVYVTATLFKPLDDGAMPLTVAHGFIPLIVSKPELKLPVA
                     ITCAAKSKSKSKQTIKVRTVPNAELTVAVVDEGILALKNQKSPDPFGYFYQKKALQTT
                     TYDVYAQLLPDLKMRSSTTGGDGFDLARRVNPLSNKRVQLVALWSGILKANAAGDAVF
                     NIEIPQFSGKLRVMSCSWKDNRFGSSEQFISVADPVVISPSVPRFLSPGDSLEMPVTF
                     TNTTSMPLTLNVSCSTTGNLKLSNNASSQLTIAPSAEARYVWNIVASSAIGQGAIEVK
                     VNTGKETFSDKTAITIRPPSSLAKETSNGVAEGNTTVKVASDFISGSAKVNFMLSASP
                     LVQFTDQLIYLFDYPHGCLEQTVSIAFPQLYYGSLAKNITNKNGVAYNPDFNIQEAIR
                     KIEGMQIYNGSMTYWPGSVIENWWATAYALHFLTEARKAGYSVNENTINRTFEYLKSK
                     VKTKETEIVYFANASNVIEKQVKVKREIIYSLYLMALNDKRDLSMMNFYKANHSQLTL
                     DSKYMLALSYLAIGDTKSYLALLPEKFAGEKSERSLAGNFSSYIRDQALTLNCLLETD
                     PDNAQIPNMARTLSQLLRGEKWLSTQERAFALLALGKFAKRSTSTNVRATVFADGRQI
                     GVFNGNDLTINSNLAGKKIEIRTEGKGQLYYFLEVSGIKESLKFKDEDSKLIVRRSYF
                     NRFGQQMNNNFKQGDLIVVQLALSSPTGNDVENVVLTDLIPSGFEIENPRIVDDREMT
                     WVKSKAQPDYFDIRDDRINLYSQAGKVTKYFYYTVRAVNTGSFVVAPVSADAMYDGEF
                     HSYSGGGRVTVVK"
     gene            5925..8243
                     /gene="pbpC"
                     /locus_tag="PKY09_08800"
     CDS             5925..8243
                     /gene="pbpC"
                     /locus_tag="PKY09_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNB12995.1"
                     /translation="MKAAVKGFRCIILKSAIAIAACMLLVYVMSWLIPLNVNIPYAVT
                     VTDSRGAVLHAFLSKDEKWRMKAGLSEINPQLVKAIIQKEDRWFYFHYGVNPVAVIRA
                     AVNNLFYQRKTSGASTITMQVSRLLNPEPRTLFNKMIEAFRAVQLECRYSKKEILEMY
                     LNLIPYGGNIEGVKAASVSYLGKLPADLSLAECVTLCIIPNRPNSLRPGYDNDLIRRE
                     RNKWLTRFERNGVFKKEIINDALNEPVEMTRQAAPALITQLAYRLKEQYPTSENIHST
                     LQASLQHKVQSLVYNYSKRKSLQSIYNASAIVIDNKNHKVVAYIGSADFKDQLHHGQV
                     DGVNAVRSPGSSLKPLIYAMAFDKGLATPMTVINDVPVNYMGYNPENYNSKFNGRVTV
                     DFALSHSLNIPAVKILNQLGVPNVTQALIKCGFTSIEKNKNRLGLSLALGGCGVRLYE
                     LTNLYSAFANSGVYQKLKFIETDSLSNDTVRIVSPGAAYLTTEILSKVTRPDLPDNYA
                     NLLRIPHIAWKTGTSYGRRDAWSIGYNKRFTVGVWLGNYNGNGVAELSGASVATPLLF
                     DIFNAIDYNSDSTWFSPPADVNFRLVCSETGLLPGVSCSNQIMDEFLPGITSNAVCNH
                     VKEFMISPDEKISYCTSCMPAAGYKKKLFSSPDAEYLAFLKSENISLNLPPPHNPACT
                     RVFAGQPPRIVSPNMQSEYLLDLNAGDKIMLQCQASPEVRNVYWYINDLFYRAAAANE
                     QLFCSPPKGRIKISCADDMGRNSDIFIKIDEL"
     gene            complement(8245..8988)
                     /locus_tag="PKY09_08805"
     CDS             complement(8245..8988)
                     /locus_tag="PKY09_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNB12996.1"
                     /translation="MNKILFLIPIYSFKNKFKPIDLKLIRSYLLGLVLSMTTLMSGAQ
                     NKVFTGGASYYAKSFEGRKTANGEIYSNYDMTCASRTLKFHTFLKVTNLKNNLVTIVR
                     VNDRGPYAKNRIIDLTEQAARIIGSYKHGITKVKLEIIQPSQNTDSIEKNFMTNQIVD
                     ADGRITIPSGYTISIWRTRDFDHALLLAKYLQQEEYIQSFYVGKKNQNGRPLYHILVL
                     NIATQQEALKLKDFWERKGFMRVRMMENF"
     gene            9069..10142
                     /locus_tag="PKY09_08810"
     CDS             9069..10142
                     /locus_tag="PKY09_08810"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNB12997.1"
                     /translation="MSTVKFNNKDAIFFPELKQRINQYFEQNKIKPTGNLKLYAKTII
                     LFTVLVALYIVLVFFTPENGWLSLLLASLLGLVMASIGFNVMHDGAHGSYSSHKWVNE
                     SMAHSLNLLGGVSFIWKQKHNINHHSYTNVEGMDDDIDIKPFIRVHEGQKKYWFHRYQ
                     HIYGLLLYGITYFFWVFYNDFNKYFSGKIAEHTHMQKMSVWDHVIFWATKIFYVLVFI
                     VFPIYNVGFIDTLLGYGVMVAVTGITIAIVFQLAHVVEDMPFVSPEGDVMKIESEWAV
                     HQLQTTTNFATKNKFISWLLGGLNFQVEHHLFPRISHIHYPSISKIVKETCLEYNVQY
                     KEFRTMRGALASHILHLRDMGRK"
     gene            10398..13577
                     /locus_tag="PKY09_08815"
     CDS             10398..13577
                     /locus_tag="PKY09_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNB12998.1"
                     /translation="MKKTITLFICLFSLSLATWAQDKTVTGKVTSSQDKLGIPGVSIL
                     EPGTTNGAVTDIDGKYSIKVKSTTTQLRFSGTGLVTRTVDIPATNALDITMAADVQKI
                     GEVVVTALGVKREEKSLGYSTQKVSGEDLSAAKEANFINSLQGRVAGVSITGSSNLGG
                     SSRILLRGMRSINFENQPLFVVDGIPMNNENVATVDQQRGALGYDYGNAIQDINPDDI
                     ESVNVLKGPSATALYGSRGSNGVIIITTKKGVAREKSEKYSPIGVTINSGWSMKKIYN
                     LPKYQNRYGGGASSDFIESDIHPGELRSEFEYDGSWGPELLGQEVYQWDSYYPSMPNY
                     NKKTPWVAHPNNVKDFFETGIVKNNSISLDGGNEKSLFRLGYTNYDEKGVIPNESLNR
                     NIISFNGSNKFNDRLTASLGMNYVVARGKGRAATGYNSIASNFNQWWQRQLDIKELKD
                     YKNPDGSQRAWNMNAEDDPSPLYWDNPYWVSYENYETDQRNRLYGGLTFSYDIGKGFT
                     LAASGKTDYYNDNRQERVAVGSAVATAIPAYSENSITFNENNYEMILSYKHRLGDDLD
                     FNGFVGANRQDQKFSNKFISTQGGLNVPNFYNINNSTSSELIYSSQVRQLRKNSLFAS
                     ASFGYQSFLYLDITGRNDWSSTLPEGKNSYFYPSITASFVFSEKLDFNWLSYGKLRAG
                     WAQTAIDPVPYRSTSTSPSMSDNFNGLPTSVIPGTFNNTDLKPEMTSGFETGLELHFL
                     QKRISFDIAYSSTRSKDVIFNVQQSGASGIDFKIYNAAEITNKTVELMIGLVPVKLNN
                     GFEWGVGMNWAKTNNEVVKLFTDELGNETESVQIGTAPFSATLQMRKGMAASQIVGYD
                     FVYDDKGNKVVDADGFYMRSETVKPLGSVLPDYTGGFNTYVTFKGIRLAGLIDFQKGG
                     KVFSLTNVWGKYSGIFAETAEGDIRENGLINEGVTETGEQNTTVIAAVDHYFQDGGYV
                     ITAADVYDASFVKLREITLSYELPQSLFKKAIRGVSIGLYGRNLAILHKNVPNIDPEA
                     GLSSGNVQGFEGGQLPTARIFGVNLNVRF"
     gene            13596..15125
                     /locus_tag="PKY09_08820"
     CDS             13596..15125
                     /locus_tag="PKY09_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNB12999.1"
                     /translation="MIKFKINQKAIATVMVFSLLLGSCKKNFESINEDPNNPKEVPNA
                     YLLTGAIRGLMDNTIDAWWGANVGNQLGQYWSSNQYSSESRYLYRSNVTNSYWRYFYG
                     GVQNDQLVAVGGLYELSQIVKKCKEDSIEASRDGYVPNQIAVATIVRVWTFQNMTDIW
                     GDIPYSEALQADANKSPKYDKQEDIYNGLLSEIDEAIAMINDAESGPNGDVVYNGDMA
                     AWKKFANSLKMRLALRIVDRNPAKAQSEFEAAFTAGAFSANSDNALLQYGEFPSGNPI
                     YYNRYISGRNDYASSNTFLDSTLTPLNDPRRECFFVPATATGLWTGEVYGLSEANAAQ
                     TPNSRIAQRSPLVLSASLPGIFMDYAQVEFMLAEAAERGWAVTGSAESHYDAGITASI
                     SFWTELNGSPASAGDIAAYIAQPTVNYQTAGGTWQQRIGVQKWIALFDQGIQGWVEWR
                     RLDFNKLMMPADGVIDGTGIPLRMKYPVLEQTLNSASYSAAVAAQGADEQDTRMWWDV
                     N"
     gene            15235..16620
                     /gene="asnS"
                     /locus_tag="PKY09_08825"
     CDS             15235..16620
                     /gene="asnS"
                     /locus_tag="PKY09_08825"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNB13000.1"
                     /translation="MSHSYIKALLNTEPIGQIVQAKGWVRTKRGNKAISFIAINDGST
                     IHNIQVVAENSSFDESLLKNITTGACIAVQGKLVASQGQGQKVEMQATQIEIYGTADP
                     ETFPLQKKGHTMEFLRDIAHLRPRTNTFGAVFRIRHTMAYAINHYFHTNGFFYIHTPI
                     ITGSDAEGAGEMFRVTTLDLNKPPRNDDGTIDYSQDFFAKETNLTVSGQLEGELAAMA
                     LGKIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFFDLNDNMDLAEDMLKYLVRYALDS
                     CMDDLEFLNNMYDKELIGRLRSVIENPFKRLTYTEAINVLEPNNSKFEYKVSWGADLQ
                     KEHENFLVEHFKTPVILTDYPAAIKAFYMKQNDDGKTVRAMDILFPWIGEIIGGSQRE
                     ENYNKLVSRMQAMHIPEKEMWWYLDTRRFGACPHSGFGLGFERLMLFVTGMNNIRDVI
                     PFPRTPRNAEF"
     gene            complement(16631..>17416)
                     /locus_tag="PKY09_08830"
     CDS             complement(16631..>17416)
                     /locus_tag="PKY09_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB13001.1"
                     /translation="TWNSSLWQVAAVIGPAMGGFFYGWIGVFYSSLVVCAFIIVTMIC
                     YLLIKDRHVDYHNKTESILQSLKTGVNFVFKNQYIISAISLDLFAVLFGGAVALLPVF
                     ADQVLHVGAEGLGLLRAAPAVGSVVMAIYLAFHPPRKDAGYKMLASVAGFGLCMIGFA
                     LSSSFVVSLLLLFMSGVFDNVSVVIRSTIIQSFTPDEMKGRVSAVNSIFVGSSNELGA
                     FESGVMAKLMGLIPSVLFGGGMTLLVVIITFLKAKKLKELNLP"
     assembly_gap    17417..17516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17619..17840)
                     /locus_tag="PKY09_08835"
     CDS             complement(17619..17840)
                     /locus_tag="PKY09_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNB13002.1"
                     /translation="MYWTLELASHLEDAPWPATKDELIDYAIRSGAPVEVVENLQELE
                     DEGESYESIEEIWPDYPTKDDFFFNEDEY"
     gene            complement(17954..18505)
                     /locus_tag="PKY09_08840"
     CDS             complement(17954..18505)
                     /locus_tag="PKY09_08840"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNB13003.1"
                     /translation="MKIYTKTGDSGKTSLIGGTRVSKHHLRIDAYGTVDELNSTIGLL
                     KDLAEEKDQCKEILAEIQDRLFTIGSSLAADPEKSKMKIPDLNTEDITLLEKEIDRMV
                     AALPPLKSFILPGGHLLNSHSHVARCVCRRAERLATGLSELEWVEPMVLIYLNRLSDY
                     LFMLARHVCFVRNITETPWRPRV"
     gene            18729..20309
                     /locus_tag="PKY09_08845"
     CDS             18729..20309
                     /locus_tag="PKY09_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNB13004.1"
                     /translation="MFKKVGLVLLLLAYCRSTSAQNIEIQKLGALLQMIDYYYVDTVN
                     KTKLVDDGIRAILKDCDPHSQYIPASELKEMNEPLVGKFEGVGIQFNILEDTIMVTQT
                     IAGGPSEKLGIRPGDRIVNIDGKKVAGVKITNKDVISKLRGDKGTKVKVDMYRRGEAE
                     LLTFDITRDKIPLFSVDASYKIEPDIGYIKISRFADNTVEEFKEALAKLKSQGVKSLI
                     LDLTSNGGGYLNRAHQLADEFLSDGKRIVYTEGRSQSREDYFSTAAGGWEKGKLVVMI
                     NEYSASASEIVSGAIQDWDRGLIVGRRSFGKGLVQKPYSLPDGSAVRLTVARYYTPSG
                     RCIQKSYQEGDEEYSDDISNRFKHGELYHADSIKFNDSLKYTTNAKRVVYGGGGIMPD
                     VFVPLDTMFSSKYYSDLIRKAALSEFALTYADNNRSKLKSAYTTVENFKKSFNVDDKM
                     MNDFIAYAEKKEVKKDEEGLKRSGAMIKSQIKALIARDIFNFGAYFYIINDSDDTFNK
                     AVESIKDNTFSKMKIAVK"
BASE COUNT         6527 a         3761 c         4091 g         6089 t
ORIGIN      
        1 ttgcccaaca accccgatta tgagttgggc gcattgcaag gtagcggttg cgatacgcta
       61 acagcattaa agcaagatcc ggaaatagct aaaaataatt tatttgttta tcccaatcct
      121 gctaaggatt ttgtcaccat tgcgttttca aaggctgttc gtacaacctg caacttacgt
      181 ttgtttaatg tactgaataa agaagtttac aggcaaaatt ttgtacaaca aaaagtaact
      241 gtacccatag caaatcttgc aaaaggcatt tacattgttg aggttagtaa caatggtttt
      301 aaagaatatg caaaatttgt aaaagagtag attcagattg tttagtaata caaagcaata
      361 aattaagtcc tttttattca gaattagttg cgttgtggaa gcatttaatc accgtattat
      421 tgttggtatc tcatttttta tttattggga atttttaaaa ttttaatccc taaatacttc
      481 tataacattt catccgttac ctttatgatt tttaatcaag gtaaaaactg cattatgagt
      541 acccttaaat accatttctg gacattactc tgtattgttc tgctatcggc atgcaaaaac
      601 caaaacacgg tttctgttgc caataaaaac tttgaagatg ttgtagagca acaacaaaac
      661 ctcattttta ctttcgataa agaccttgtt gacgatagcc tgcttaaccg ttgggacagc
      721 acacagtttg ttgctttcac accagcggtc aaaggattgt ttaagtggaa caccaaacgc
      781 gagttgatgt tttcgccaga aacatctttt ggccctgcta cattttataa agctgttgtt
      841 acagataaaa ttctttttga aaacaggtta ctttctttaa atgtacctgc agcatatgat
      901 tttcatacac cactgacaca acttgaaggc cttcatgcac actgggcgct atctacacaa
      961 caaaacagca ttgtattact tgccaaactt gattttaatt atcctgttcc gtcagctcag
     1021 atgaaagacc ttgctcagat aaagattaat gataaagttt atccttttac cgttgttacc
     1081 aacgatgctt caacagaagt agttctggaa ataaacaaca tgcagcctgc agattttaaa
     1141 aaagtaaatg tcagcgtatc tattaataaa ggtttggcat gtaatggcag caattataaa
     1201 actgaaagtg ttcttattaa agatgcagat gtgccactac ccaacaggct caccgttatg
     1261 aatgcatcag gcgagtatga taacacacaa cctgttattc atgtttacac caatcaggca
     1321 gttgatatga atcagattgc aatgtatgtt actgttcagc caagagtaga ttttaccaca
     1381 gaagcagcca acgatggctt cttagtaaag ggtgcttttg acccgggaca gtcgtatcag
     1441 cttacagtta aaaaagatct gaaaggatta attggcggca tgctggagca ggattttatt
     1501 gcaactgtac cttttggcga aatgcagcca tccatatcat ttaccaacgc tgctggtatg
     1561 tatctttctt caaagggaaa tagaaatata ggaatacagc tcgtaaacgt gcctaaagtt
     1621 cgtgtaagcg tttataaagt ttatgaaaac aacattctga attttatgcg tcaaaacagt
     1681 tatagcaatt actatgaaga atcagatgat ggtagctacg gtggtaacca atataatatt
     1741 tatggaattg aaaattatgg cgacttagtg atgcaacgta attacagtgc caaaagtctg
     1801 cctcgcggta acggcatcaa tttgctcaat ctttctctcg atgaaattaa tgcctacaaa
     1861 ggcatttatg tggttgatgt tgcatcaact gacgattatt ggctgcacga tacaaaagta
     1921 attgcacttt ctgatattgg aatgatagca aaatattccg aaaacgaatt gacagtcttt
     1981 accaactata ttaaatcggc cttgcctgcc gaaggtgtaa atgtcagttg catatcaacc
     2041 aacaatcagg tgctgttcaa cggcacaaca ggccatgatg gaaagattac tttttctgat
     2101 ataggcagta aagcaaaaaa ttttcgtgta ggaatgattt ctgccagaca gggtaaagat
     2161 tttaatatga ttttgctgag tgaatcgaag gtgagtacaa gccgctatga tgttggtggc
     2221 tataaagata atcctgccgg agttatggct tacatttatg gcgacagaga tatgtatcgc
     2281 ccgggcgaaa cggtttatct gaacacaata gtgcgtaatg aaaaatggat gcccgttgcc
     2341 aatgtacctg ttaaattgaa aatgaccatg cccaatggtc atgagtttaa gacactgcgt
     2401 aaatccctca acagtcaggg tgcagtggca gcagattttt tattgccgca ggcagcagtt
     2461 acaggaaatt ataatataga tattttttct gccaacgatg tacttatcgg ctcaagaaca
     2521 atacaggtag aggagtttat gcccgacaga attgatgtga aagtaaaaac taataaaaac
     2581 aattttcaac ctgctgacac cattattgct gcaattgctg ctacaaattt tttcggtccg
     2641 ccagcagcaa tgcgcaagtt tgaagcagag tttaatctcg taagaaaaag ttttttaagt
     2701 aaagaattca gtggctataa ttttactgtt aaaacaatta acgaactacc ggtagataaa
     2761 aaggttgagc aagggcagac aaatgaaaaa ggcgaagcaa cagctaagtt tctgattccc
     2821 gattcctgta aaaactcagg ccttttgcaa ggtagaattt acacaacggt gtttgatgaa
     2881 accggacgtc cggtaaacag gctcaacact tttgatgtaa atacgcaaag aaattatttc
     2941 ggtattaagc tgaatgatta ttatgcaaat attggcacca acatggtgat taatctggca
     3001 gcatgtaata gcaatggcag tggtgcagcg gctacggcct atgtagagat aattaattac
     3061 gattggtaca atgtgattga aaaatcagat aacggacagt atcgctatgt ttcgcaaaag
     3121 cgcgaagtaa ccatgttaag ccgcgaaata aatattgctg ctggcggaag taatctgaat
     3181 tttattcctg ccaattcagg tgagtatgag gtgcgcatca gtgcagttaa aggtggtaac
     3241 tatgtggcat caaatttttg ggcttatgga tatggttaca catcaaacac atcttttaac
     3301 ataaacacag aagggcaggt tatcattaag gcagataaag acacatttac accgggcgaa
     3361 aaagctaact tactctttaa agcacctttt gcaggtaagt tattagttac ggttgagagc
     3421 aacaggcttt taaaacatta ttatatcaac acagataaac gttcggcaat gctttcaata
     3481 cctgtggacg aaagtttctt gcccaatgtg tatgtaacag ctacgttgtt taagccactt
     3541 gatgatggcg caatgccatt aacggtagcc catggcttca taccccttat tgtttcaaag
     3601 cctgaactga aattacctgt tgccataact tgtgctgcaa agtcaaagag taagagtaaa
     3661 cagacgatta aagtacgcac tgtccctaat gcagaactga ctgttgctgt tgttgacgaa
     3721 ggtattcttg cattaaagaa tcagaagtca cctgatccat tcggatattt ttatcagaaa
     3781 aaagcattgc agactacaac gtatgatgta tatgcgcagt tgcttcccga tttaaaaatg
     3841 cgttcatcca ccaccggtgg tgatggattt gatctggcaa gaagagtaaa tcctttatcc
     3901 aacaaacgtg ttcagttggt agcactttgg agtggcatac tcaaagccaa tgcagccggt
     3961 gatgcagtat tcaacattga aattccacag tttagtggca agctacgtgt aatgtcatgt
     4021 agttggaaag ataaccgctt cggaagcagt gagcagttta tcagtgttgc cgatcctgtg
     4081 gttatcagtc cgtctgttcc acggtttcta agtcccggtg attcgttaga gatgcctgta
     4141 accttcacca acactacgtc aatgccttta acgttaaatg taagttgttc tacaacaggg
     4201 aatcttaaat tgtccaataa tgcatcatca caactcacaa ttgcaccctc agcagaagcg
     4261 cgttatgtct ggaatatagt agcttcatcg gccatagggc agggtgcaat agaagttaaa
     4321 gtaaatacgg gtaaagaaac attttcggat aagactgcaa tcaccatcag accgccatct
     4381 tctttagcaa aggaaacaag taatggtgtg gccgaaggaa acactactgt aaaagttgca
     4441 tctgatttta tttccggttc agccaaagtg aattttatgc ttagtgcatc accgttggtt
     4501 cagtttacag atcagctgat ttatttattc gattatccgc atggctgttt ggaacaaact
     4561 gtctcaattg catttccaca gttgtattat ggtagtcttg caaaaaacat caccaacaaa
     4621 aatggtgtgg catataatcc tgactttaat attcaggaag ctatcagaaa aattgaaggc
     4681 atgcagatat ataatggctc aatgacatac tggccgggta gtgtaattga aaactggtgg
     4741 gcaactgctt atgccttgca ttttctgact gaagcaagaa aagcaggata ttcagttaac
     4801 gagaacacca tcaacagaac ttttgaatat ctcaagtcaa aagtaaaaac taaagaaaca
     4861 gagatagtat attttgctaa tgcaagtaat gttattgaaa agcaggtgaa agtaaaacgt
     4921 gaaattatct attcacttta tctgatggca cttaacgaca agagagattt gtctatgatg
     4981 aatttttata aggcaaatca ttcacaactg acattggatt caaaatacat gcttgcctta
     5041 agctatcttg caattggcga tacaaaaagt tatttggcat tattacccga aaaatttgca
     5101 ggagaaaaat cagaaagaag tcttgcaggt aatttctcat cctacatcag agatcaggca
     5161 ttgactttaa actgccttct cgaaaccgac ccggacaatg cacagattcc aaatatggcg
     5221 cgcacacttt cgcaattgct aagaggagaa aagtggttga gcacgcagga acgtgcattt
     5281 gctttgcttg cattgggtaa gtttgctaaa cgcagtacat caacaaatgt tcgtgcaaca
     5341 gtgtttgccg atggaagaca gattggtgtt tttaatggca atgatttgac gataaatagc
     5401 aatcttgccg gaaagaagat tgaaatacgg acagaaggca aaggtcagtt gtattatttt
     5461 cttgaagtga gtggaataaa agaaagctta aagtttaaag atgaggatag caaactgatt
     5521 gttaggagaa gttacttcaa tcgctttggt cagcaaatga ataacaattt taaacaaggt
     5581 gatttgattg tcgtgcagtt ggctttaagt tctccaaccg gtaatgatgt agaaaatgtt
     5641 gtacttacag atttaattcc ttcaggattt gaaattgaga atccacgaat tgttgacgac
     5701 agagaaatga catgggtaaa aagtaaagcg caaccagatt attttgatat ccgtgacgat
     5761 agaattaacc tatattctca ggcaggtaaa gtaacgaagt atttttatta cactgtgcgc
     5821 gctgtaaaca ccggaagttt tgttgttgcg cctgtcagcg ctgatgccat gtatgatgga
     5881 gagtttcatt cttatagcgg aggtggaaga gttacggtgg ttaaatgaag gcagcagtta
     5941 aaggcttccg atgtattatt ttaaaaagtg caattgctat tgcagcatgc atgttgttgg
     6001 tgtatgtgat gtcatggttg attccattga acgtaaacat tccttatgct gtaacagtta
     6061 ctgacagtcg tggcgcagtg ctgcatgctt ttttatccaa agatgagaaa tggaggatga
     6121 aagcaggttt gagtgaaata aatccacaat tggtaaaagc tattattcaa aaggaagaca
     6181 ggtggtttta ttttcattat ggcgtaaatc ccgttgccgt tatcagggca gcagtaaata
     6241 atttatttta ccagcgaaag acttcgggtg catcaaccat tacaatgcag gtttcaagat
     6301 tgcttaatcc tgaaccacgc acattgttta ataaaatgat tgaagctttc cgtgcagtac
     6361 aattggaatg caggtattca aaaaaagaaa ttttagagat gtatctcaac ctgattccgt
     6421 atggcggaaa tattgaaggt gtaaaagctg catcagtttc atatctcggc aaattgcctg
     6481 ccgacttatc acttgcagag tgtgtaacat tatgcatcat tcctaacaga ccaaattctt
     6541 tacgcccggg ttatgacaat gatttaataa gacgtgaaag aaataaatgg ctgacaagat
     6601 ttgaaaggaa tggagttttt aaaaaggaaa tcatcaacga tgctttgaat gaacctgttg
     6661 aaatgacacg acaggctgca cctgcattga taacacaatt agcctatcgc ctgaaggaac
     6721 agtatccaac ttcggagaat attcattcta ccttacaagc atcgttacag cataaagtgc
     6781 aatcgcttgt ttataattac agtaaacgta agtcattgca aagtatttac aatgcatcgg
     6841 caattgtgat tgacaataag aatcacaaag tagtagccta tattggctcc gcagatttta
     6901 aagatcagtt acatcacgga caggttgatg gagtaaatgc tgttcgttct ccgggtagca
     6961 gcctcaagcc attaatttat gcaatggcat ttgacaaagg tttagcaaca cctatgacag
     7021 taattaatga tgtgccggta aattatatgg ggtataatcc tgaaaattat aattcaaaat
     7081 ttaatggtag agttactgta gattttgcat tgtcacattc actgaatatt cctgcagtaa
     7141 aaattctgaa tcagttgggt gtaccaaatg taacacaggc attaattaaa tgtggtttca
     7201 cttcaattga aaagaataaa aacagactgg gattatcttt ggctcttgga ggttgcggtg
     7261 tgcgccttta tgaacttaca aacttgtatt ctgcctttgc aaatagtgga gtttatcaga
     7321 aattaaaatt tattgaaacg gattcattga gtaatgatac tgtcagaata gtttctcccg
     7381 gtgcagccta tctgactaca gaaatactta gtaaggttac gcgccccgac ttgcctgata
     7441 attatgcaaa cctacttcgt attcctcaca ttgcatggaa aacaggaaca tcgtatggcc
     7501 gaagagatgc atggagcatt ggctataata aaaggtttac tgttggtgta tggctgggaa
     7561 attataacgg caatggcgtt gccgaactta gtggagcttc tgtggccacg cctctgctct
     7621 tcgatatttt taatgcaata gattataaca gtgactcaac atggttcagt ccgcctgccg
     7681 atgtaaattt cagattggta tgcagcgaaa caggtctgct tcccggtgtc agttgcagca
     7741 atcagattat ggatgagttc ttacccggca taacctccaa tgctgtttgc aaccatgtaa
     7801 aagaatttat gatttctcct gatgagaaaa taagttattg cacatcatgt atgccggcag
     7861 caggttataa gaaaaagctg ttttcttctc ctgacgctga atatctggca tttctgaaaa
     7921 gtgaaaacat cagtctcaat cttccgccac cgcataatcc tgcttgcacc agagtatttg
     7981 cagggcagcc tccaagaatt gtttctccca atatgcaaag tgaatactta cttgatttaa
     8041 atgcaggaga taaaattatg ctgcaatgtc aggcatcacc ggaagtaaga aatgtttact
     8101 ggtacattaa tgatttgttt tatcgtgctg cagcagctaa tgagcaactg ttttgttctc
     8161 caccaaaagg ccgtattaaa atttcatgtg ctgatgatat gggccgcaac agcgacatct
     8221 ttattaaaat agacgagttg taattcaaaa attttccatc atccttacac gcataaagcc
     8281 tttccgctcc caaaaatctt ttaacttaag tgcttcttgc tgtgtggcaa tattcaaaac
     8341 taaaatatga tacaacggtc tgccattttg attttttttg ccaacataaa aagactgaat
     8401 gtattcttct tgttgcaaat attttgcaag tagcagtgca tgatcaaaat cgcgtgtgcg
     8461 ccaaatactg atagtgtaac ctgaaggaat tgtaattcgg ccatctgcat ctacaatttg
     8521 atttgtcatg aaattttttt caattgaatc ggtattttgc gatggctgaa taatttcaag
     8581 cttcactttt gttatgccat gtttatagct tccgataata cgtgctgcct gttcagtaag
     8641 gtcaatgatg cggttttttg catatgggcc tctgtcatta acacgaacaa tggtaactaa
     8701 gttgtttttc agattggtta cttttaaaaa agtgtgaaac tttaatgttc tgctggcaca
     8761 ggtcatgtca tagttgctgt atatttcgcc attggctgtc tttcggcctt caaagctttt
     8821 tgcataatat gaagcaccgc ctgtaaacac tttgttttgt gcaccggaca ttaacgttgt
     8881 catgctcaaa accaaaccca ataaatagct tctaatcaat tttaaatcaa taggtttaaa
     8941 tttattctta aatgaataaa ttggtataag aaatagaatt ttgttcatct ttttaagtgt
     9001 atttcttttt cagaaccaag caaagtacac gttcaaaata cctacatttg cactcaatat
     9061 ttaatatcat gtcaacggta aaattcaaca ataaagatgc aatatttttt cctgagttaa
     9121 agcaacgcat caatcaatat tttgaacaaa ataaaataaa gcctaccggt aatctgaagt
     9181 tgtatgctaa aaccattata cttttcaccg ttttagtggc actttatatt gtattggtat
     9241 tttttacccc cgaaaacgga tggttgtctt tactattagc ttcactctta ggtttggtaa
     9301 tggcatctat tggctttaac gtaatgcacg atggcgcaca tggcagctac tccagtcata
     9361 aatgggtgaa tgaatctatg gcacattcac tgaatttact gggtggtgtt tcatttatct
     9421 ggaaacaaaa gcataacatc aatcatcatt cttataccaa tgttgaagga atggatgatg
     9481 atatagacat caaacctttt attcgtgtgc acgaaggtca aaagaaatat tggtttcatc
     9541 gttaccagca tatttatggc ttattgctgt atggaataac ttatttcttc tgggtttttt
     9601 ataatgattt taataaatat ttttcaggta aaatagctga acatacccac atgcagaaaa
     9661 tgtctgtttg ggatcatgtt attttctggg ctactaaaat attttatgtg ttggtgttta
     9721 ttgtattccc aatatataat gtagggttta ttgacacact attaggttat ggcgtgatgg
     9781 tggcagttac aggaattact attgccattg tttttcaatt ggcacatgtt gttgaagata
     9841 tgccatttgt aagccccgaa ggtgatgtaa tgaaaattga gtcggagtgg gcagtgcatc
     9901 aattgcagac aactaccaac tttgcaacaa aaaataaatt tatcagttgg ttgttaggtg
     9961 gtttgaattt tcaggttgaa catcatttat ttcctcgtat aagccacata cactatccat
    10021 caatcagcaa aattgttaaa gaaacttgtt tggagtataa tgtacaatac aaagagtttc
    10081 gtaccatgcg tggtgcattg gcatcgcata ttcttcatct tcgcgatatg ggtagaaaat
    10141 aggctgcatt attttagcct gtttaactca gttattacct gagtactgta aatttttagt
    10201 taaaatttgg ttaattcaaa ttattattaa ctttgtgtga agtttttctg aaaccacata
    10261 catgaagttg tttttcataa aaggttattc gcaatataaa aaagatctgt ttttcataga
    10321 cacagacagt agattaaacc gccagtttca ttctacaaca aataaattaa ataagttaaa
    10381 ctcaaattaa aagcagtatg aagaaaacaa tcacattgtt tatttgcctg ttctcgttgt
    10441 cattggcaac ctgggcacag gacaagactg taaccggaaa ggttacttca tcgcaggaca
    10501 aactgggtat tcccggtgtt agcatcctcg agcccggaac taccaatggt gccgttacgg
    10561 atattgatgg aaaatattcc ataaaggtaa aatctactac aacccaactc cgtttttccg
    10621 gaacaggttt agtaacccgc acagtggata tacctgccac aaatgcactt gacattacta
    10681 tggcggccga tgtacagaaa attggtgagg tagttgtaac ggctttaggt gttaagcgcg
    10741 aagaaaagtc tttaggttac agcacacaga aagtaagtgg agaggacttg tcggctgcaa
    10801 aagaagccaa cttcatcaac tcacttcaag gtcgtgttgc cggtgtaagc attacaggtt
    10861 ccagcaatct gggtggttct tcaagaattt tgttacgcgg tatgcgttcc atcaattttg
    10921 aaaatcaacc tttgtttgtt gttgacggaa ttccaatgaa taacgaaaac gtagccaccg
    10981 ttgaccagca acgaggtgca ttaggttatg attatggaaa tgctattcaa gatattaacc
    11041 ctgatgacat tgagtctgtt aacgtactta aagggccatc tgcaactgca ctttatggtt
    11101 caagaggatc aaacggtgta attattatca caactaaaaa aggcgttgcc cgcgaaaaat
    11161 ctgaaaagta ttcaccaata ggtgtaacaa ttaattcagg ctggagcatg aagaagattt
    11221 ataatcttcc taaatatcag aaccgttatg gtggaggagc atcttccgat tttattgaga
    11281 gtgatattca cccgggcgag ttgcgctctg agtttgaata tgatggtagc tggggtcccg
    11341 aattgcttgg acaagaagtt tatcagtggg atagctacta tccttcaatg cctaattata
    11401 ataagaaaac tccatgggta gcacatccaa acaacgtaaa agatttcttt gaaaccggta
    11461 ttgttaaaaa taacagcatt tcgctcgatg gcggtaacga aaaatcactt ttcagattag
    11521 gttataccaa ctatgacgaa aaaggtgtta ttccaaacga aagcctcaac agaaatatta
    11581 taagctttaa tggctcaaac aaatttaacg acaggcttac agcttcatta ggcatgaatt
    11641 atgttgttgc cagaggtaaa ggacgtgctg caacaggata caacagtatt gcttctaact
    11701 tcaaccagtg gtggcaacga caactcgaca tcaaggagct taaagattat aaaaaccctg
    11761 atggctcaca gcgtgcatgg aatatgaatg cagaagatga tccatcacca ctatattggg
    11821 ataatccata ttgggtttct tacgagaatt atgaaaccga tcaacgtaat cgtttgtatg
    11881 gtggattgac tttttcttat gatattggta aaggatttac acttgctgca tcaggaaaaa
    11941 cagattatta taacgacaac cgtcaggaac gtgttgccgt aggaagtgcc gttgcaacag
    12001 caatacctgc ttattcagaa aacagcatta ccttcaatga gaataactac gaaatgatac
    12061 tttcatacaa acatcgtttg ggtgatgatt tggattttaa tggctttgtt ggtgcaaaca
    12121 gacaagatca gaaattcagc aataaattta tttctacaca gggtggttta aatgttccaa
    12181 atttttacaa catcaataat tcaacaagca gcgaattaat ttattcttcg caagtaagac
    12241 aacttcgtaa aaattctctt tttgcaagtg catctttcgg atatcagagt ttcttatacc
    12301 ttgacattac tggacgtaat gactggagtt caacattacc tgaaggcaaa aattcatact
    12361 tctatccatc aattacagca agttttgttt tctccgaaaa attggatttc aactggttga
    12421 gttatggtaa actgcgtgca ggttgggcac aaacagctat tgatcctgtt ccttatcgtt
    12481 caacaagcac ttcaccaagt atgtctgaca attttaatgg attacctaca agtgttattc
    12541 cgggtacatt taacaatact gatttaaaac cggaaatgac ttcaggtttt gaaacgggat
    12601 tagaattaca tttccttcaa aaacgaatct cattcgatat tgcttatagt tcaacacgct
    12661 ctaaagatgt aatcttcaac gtacaacaat ccggtgctag cggcatagac tttaaaattt
    12721 acaatgctgc cgaaataacc aacaaaacag ttgaattgat gattggtctt gttcctgtta
    12781 aattaaataa tggatttgag tggggtgttg gcatgaattg ggcaaaaacc aacaacgaag
    12841 tggttaagct gtttacagat gagttaggta atgaaactga aagtgtacag attggtacag
    12901 cgcctttctc tgcaacgctt caaatgcgca agggcatggc agcttctcag attgtaggtt
    12961 atgattttgt ctatgatgac aaaggaaaca aagttgttga tgccgatggt ttctatatgc
    13021 gttccgaaac agttaaacca ttaggttcag tattgcctga ctatactgga ggttttaata
    13081 cctatgtaac atttaaaggc attcgtcttg ctggattaat tgacttccaa aaaggtggta
    13141 aggttttctc tcttaccaat gtatggggaa aatattcagg aatttttgct gaaacagccg
    13201 agggcgacat ccgcgaaaac ggtttgatta acgagggtgt aactgaaaca ggcgaacaaa
    13261 acacaacagt aatagctgcc gttgatcact attttcagga tggtggttat gtaattacag
    13321 cagccgatgt atatgatgca tcattcgtta aacttcgtga gattacattg tcttatgaat
    13381 tgccacagtc attattcaaa aaagcaatac gtggtgtgtc aattggtctt tacggtcgta
    13441 accttgccat ccttcacaaa aacgtaccaa acattgaccc tgaagcaggt ttgtcaagcg
    13501 gcaacgttca aggatttgaa ggtggtcagt taccaactgc aagaattttt ggtgttaacc
    13561 tgaatgtaag attttaactt taaaaacgag agatcatgat aaaatttaag ataaatcaaa
    13621 aagcaattgc aacggtgatg gtattctcgc tgttgctggg ttcatgtaaa aagaattttg
    13681 aatccataaa cgaagacccg aataatccca aagaagtacc taatgcttat ctgctaacag
    13741 gagccattcg aggattaatg gataatacca ttgatgcgtg gtggggtgct aacgttggta
    13801 atcagttggg tcagtattgg tcttcaaacc agtattcctc agagagtcgc tatctctatc
    13861 gttctaatgt taccaattca tattggcgat atttttacgg tggcgtacaa aacgatcagt
    13921 tagttgctgt tggcggattg tatgagctga gtcagatagt taagaaatgt aaagaagatt
    13981 caatagaagc ttctcgtgat ggttatgtcc ccaatcagat tgcagttgca accattgtcc
    14041 gtgtttggac attccaaaac atgactgata tatggggtga tattccttac tcagaagcct
    14101 tacaggccga tgccaacaaa tcacctaagt atgataagca ggaagacatc tacaatggtc
    14161 ttttaagcga gattgatgaa gctatcgcaa tgataaacga tgctgaatca ggacccaatg
    14221 gtgatgttgt atataatggt gatatggctg catggaagaa gtttgccaat tcattaaaga
    14281 tgcgtttagc tttaagaatt gttgaccgta atccggctaa agcacagtca gagtttgaag
    14341 cagctttcac tgcaggtgcc tttagcgcca acagcgacaa tgcattatta caatatggag
    14401 aatttccaag cggcaaccct atttattata accgctacat ctccggtcgt aatgactatg
    14461 cctcaagcaa tactttcctc gattctactt taacaccgtt aaacgatccg cgcagagaat
    14521 gtttcttcgt acctgccaca gcaaccggtc tttggactgg cgaagtatat ggcttgtctg
    14581 aagccaatgc tgcacaaaca ccaaactcac gtattgcaca acgttctcct ttggttttat
    14641 ctgcttcatt gcccggcatt ttcatggact atgctcaggt agaatttatg ctggccgaag
    14701 cagctgaaag aggatgggct gttacaggtt ctgcagaaag tcattatgat gcaggtatta
    14761 ctgcaagcat ttctttctgg acagaactta atggaagccc tgcatctgcc ggtgatattg
    14821 ctgcctacat tgcacaacct actgtaaatt atcaaacagc cggaggcact tggcaacaac
    14881 gtattggcgt tcagaaatgg attgctttat ttgatcaggg tattcagggt tgggttgaat
    14941 ggagaagact tgacttcaac aaactcatga tgcctgccga tggtgttatt gacggaacag
    15001 gtattccttt gcgaatgaag tatcctgttt tagagcaaac actaaatagt gcaagctaca
    15061 gtgctgcagt tgctgcacag ggagccgatg aacaagacac ccgcatgtgg tgggatgtta
    15121 actaatagtt taatttaaaa tatatttaag gcggagttaa tctccgcctt ttttattagt
    15181 aaccactcac cattttatac tttctgataa ctttgcagca aactttactg cataatgagt
    15241 cacagttata ttaaagcttt attaaataca gaaccaatag gtcagattgt tcaagcaaaa
    15301 ggttgggtgc gtacaaaaag agggaacaaa gccatttcat ttattgccat aaacgatggc
    15361 agtactattc ataatattca ggttgttgca gagaatagca gctttgacga atcgctactg
    15421 aaaaatataa ctacaggtgc atgtattgct gttcagggta aacttgttgc ctcgcaaggg
    15481 caggggcaaa aggtagagat gcaggccaca caaatagaaa tttatggcac tgccgaccct
    15541 gaaacatttc cactgcagaa gaaaggtcat accatggaat ttcttcgtga tattgcccac
    15601 ctgcgtccaa gaacaaacac ttttggtgca gttttcagaa taagacatac aatggcctat
    15661 gccatcaatc attattttca taccaacgga tttttttaca tccatacacc aatcattaca
    15721 ggcagcgatg ctgaaggtgc aggagaaatg ttcagagtaa caacgttaga ccttaacaaa
    15781 cctccccgca atgatgacgg aactattgac tacagccagg atttttttgc aaaagaaacc
    15841 aaccttacag taagtggaca acttgaaggt gaattggctg ctatggcatt aggcaaaatt
    15901 tacaccttcg gccctacctt tcgtgccgaa aacagcaaca caccacgcca cttagcagag
    15961 ttctggatga tagaacctga aatggctttc ttcgacctga acgacaacat ggatcttgca
    16021 gaagatatgc tcaaatatct ggtacgctat gcactcgata gctgtatgga tgatttggag
    16081 tttctgaata atatgtacga taaggaactg attgggcgtt tacgcagtgt catagaaaat
    16141 ccgtttaaac gcttaaccta caccgaagca ataaatgttt tagaaccaaa caacagtaag
    16201 ttcgaatata aagttagttg gggtgccgac ctgcagaaag aacatgaaaa ctttttagta
    16261 gagcatttta aaacacctgt tattttaaca gattatcctg ctgctatcaa agcattttat
    16321 atgaagcaga atgatgatgg aaaaactgtc agagcaatgg atattttatt tccatggata
    16381 ggcgaaatta ttggcggctc gcagcgcgaa gaaaattaca ataagttagt cagccgtatg
    16441 caagccatgc atattcccga aaaagaaatg tggtggtatc tcgacacccg aaggtttggt
    16501 gcttgcccgc actcaggctt tggcttgggc ttcgaacgcc tgatgctttt cgtaacgggc
    16561 atgaataaca ttcgcgatgt cattcctttt ccgcgcacac ctcgcaatgc agagttttag
    16621 tttttgctga ttaaggtaaa tttaattcct taagtttttt tgctttcaga aaggtaatta
    16681 ttaccacaag caaggtcata cctccgccaa agagcacaga gggtattaag cccatcagct
    16741 ttgccataac gcccgactca aatgcgccca actcatttga cgaaccaaca aaaatactgt
    16801 tcactgccga cacccttccc ttcatctcat caggcgtaaa actttgaata atggtagaac
    16861 gaatcacaac actcacatta tcaaaaacac cactcataaa caacagcaaa agcgacacaa
    16921 caaaacttga tgaaagtgca aagccaatca tgcacaatcc aaaacccgca acactggcca
    16981 gcatcttata ccctgcatct tttctcggag ggtgaaaggc taaatatatt gccatcacca
    17041 cagagcctac tgccggtgcc gcacgcaaca aacccaaacc ttctgcacct acatgcaata
    17101 cctgatcggc aaaaacaggc aataaagcta ctgcaccacc aaacaacacc gcaaacaaat
    17161 ccagcgaaat agctgaaatg atatattgat ttttaaaaac aaaattcaca cctgttttta
    17221 aagattgtaa aatagattct gttttattgt gataatcaac atgcctgtct tttatcagca
    17281 ggtaacaaat catggtaact atgataaaag cgcagaccac aagcgaagaa taaaaaacac
    17341 caatccaacc atagaaaaaa cctcccatag caggacctat cactgcggct acctgccaaa
    17401 gactgctgtt ccatgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctt
    17521 tatgtttcca ggctacaaat gcatttttag tttttgtcat aaaaaattaa ggccaagcat
    17581 taatgcttag ccttattttc agatatcatt tataaagatt aatactcatc ttcgttgaaa
    17641 aagaagtcat ccttagttgg ataatcaggc caaatttctt caatactttc atacgattca
    17701 ccttcatcct caagctcctg aagattttca accacttcta cgggtgctcc tgaacggatg
    17761 gcataatcaa tcaattcgtc tttagtagca ggccaggggg cgtcttcaag atgcgatgct
    17821 aattccagtg tccaatacat tacaatattc ttattaaaaa attaaactaa ttgtgcctta
    17881 atttttaagg gctgcaaaaa tatgtaaatt aaattggaaa aaacaagtgt ttttttgaat
    17941 agttattaag attctaaact cttggcctcc agggagtttc tgtaatattt cggacaaagc
    18001 atacatggcg tgccagcata aaaagatagt cagaaagcct gttaaggtag attagtacca
    18061 ttggctctac ccattccaac tcactgagac cggttgccaa acgctctgcc ctgcgacaaa
    18121 cacagcgtgc cacatgactg tggctgttta aaagatgacc accaggcaat ataaatgact
    18181 tcagtggtgg cagtgcagct accatcctgt caatttcctt ttctaacagg gtaatatctt
    18241 ctgtattcaa atcaggaatt ttcattttcg atttttccgg atcagcagcc aatgaagagc
    18301 caattgtaaa aagtctatcc tgtatttcag ccaatatttc cttacattgg tctttctctt
    18361 cggcaagatc cttcagtagt cctatggtag aatttaactc atcaacagtg ccgtaggcat
    18421 caatacgcag atggtgtttg cttactcttg tgcctccaat gagtgatgtt ttgccactgt
    18481 cgcccgtttt ggtataaatt ttcattgatt aatcagataa ataacaattt ttgtacaaca
    18541 aacctattaa caatttgtta ctgaaaacaa gtgacagaag ttaaaatgtt aacagcttag
    18601 gctgttttct ttgataatta aattaaagtt tgttaaacta tcatacagct tgttgcatac
    18661 tacaaaccgc atggattaat tttggtatgt ttactttctg taacaacgga tatttaaagt
    18721 tcaatataat gtttaaaaaa gtaggattag tattattact gttagcgtat tgccgaagta
    18781 catctgcaca aaacatcgaa attcaaaagt taggtgcatt gcttcagatg attgattatt
    18841 attatgtgga caccgttaac aaaacaaaat tggttgacga tggcatcaga gctattttaa
    18901 aagattgcga tccgcattca cagtatattc cggcaagcga gttaaaagaa atgaacgagc
    18961 cattggtagg taagttcgaa ggtgtaggaa ttcaattcaa tattcttgag gatactatta
    19021 tggtaacaca aaccattgca ggtggacctt cagagaaatt aggtattcga cccggtgacc
    19081 gaattgtaaa tattgacggg aaaaaagttg caggagtaaa aatcaccaat aaagatgtta
    19141 tcagcaaact cagaggcgac aaaggcacta aagtaaaagt tgatatgtac agacgtggcg
    19201 aagcagaact tttaactttt gatatcacac gcgataaaat tccattgttt agtgtagatg
    19261 catcctataa gatagaaccc gatatcggat acattaaaat aagccgtttt gccgataata
    19321 ctgttgagga gtttaaggaa gcacttgcca aattaaaatc gcaaggagta aaaagcctga
    19381 tacttgattt aacaagcaat ggtggcggat atctgaatcg cgcacatcag ttagccgatg
    19441 aatttttaag tgacggaaaa cgcattgtct atacagaagg tcgctcacaa tccagagaag
    19501 attacttttc tactgcagca ggcggatggg agaaaggcaa acttgttgtc atgataaatg
    19561 agtactctgc atcagcaagc gaaattgttt caggggccat acaagattgg gacagaggat
    19621 taatagtcgg ccgcagatcg tttggtaaag gtttggttca aaaaccctac tcactacccg
    19681 atggcagtgc tgtacgactt accgttgcaa gatattatac accttcaggg cgatgcatac
    19741 aaaagtccta tcaggaaggc gatgaagaat acagtgacga catcagcaac cgttttaaac
    19801 atggcgaact ctatcatgcc gacagtatta aatttaatga ttcacttaaa tacactacca
    19861 atgccaaacg tgtggtgtat ggcggaggag gtattatgcc cgatgtgttt gtgcctttag
    19921 ataccatgtt cagctctaag tactattctg atttaatacg caaagcagca ttaagtgagt
    19981 ttgcattaac atacgcagat aataaccgca gtaaattaaa gtcggcttac acaacagtag
    20041 aaaattttaa aaagagtttt aatgttgacg ataaaatgat gaatgatttt atcgcttatg
    20101 cagaaaagaa agaagtgaaa aaagacgaag aaggtttaaa gcgttcagga gccatgatta
    20161 aatcgcagat taaagcactc attgcccgcg atatttttaa tttcggtgcc tacttctaca
    20221 tcataaacga tagtgacgat acttttaaca aagcagtaga atccattaag gataatacct
    20281 ttagtaaaat gaaaattgct gtaaagtaag ttacaattaa aataataatg tgtttttagt
    20341 ctttaaccat cagtgatttc tctttaactg atggttgata ttgccttact gttcaatgtt
    20401 gcactacaaa tttcaatgtt tctgtttttc cgtggtgtat tacctttgcc atatacatgc
    20461 catcaggctc atgactgaaa tccggtgtat gtaatgtgct gttaaagttt acctgttcat
    20521 acactgtgcg gccataaaca tctactatta ccaatctttc tgctttta
//
LOCUS       DAOQNP010000059        20377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_904_length_20377_cov_7.594134, whole genome shotgun sequence.
ACCESSION   DAOQNP010000059 DAOQNP010000000
VERSION     DAOQNP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 20377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_20377_cov_7.594134"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..291
                     /locus_tag="PKY09_08850"
     CDS             163..291
                     /locus_tag="PKY09_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13005.1"
                     /translation="MSDTLAPTEYRSKQKNAIEPLDELNQNNFILFIRDRLQKCIT"
     gene            288..920
                     /gene="msrA"
                     /locus_tag="PKY09_08855"
     CDS             288..920
                     /gene="msrA"
                     /locus_tag="PKY09_08855"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNB13006.1"
                     /translation="MKGLIALIMFVGCQNHAQPSQENSNFTNMETNIATDTALFGTGC
                     FWCTEAVFETIDGVISATSGYSGGTVANPSYQQVCMGNTGHAECIQIVFDPSKITYDE
                     LLEVFWESHDPTTLNRQGADVGTQYRSVIFFYNDMQKQTAENYKKKLNEENAFGKPIV
                     TEITAAQQFYPAENYHQEYFKLNGHEPYCQFVIQPKVEKIRKIFSSKIKQ"
     gene            complement(927..1814)
                     /locus_tag="PKY09_08860"
     CDS             complement(927..1814)
                     /locus_tag="PKY09_08860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNB13007.1"
                     /translation="MEHFRESLLILISTPIYLVVIGAEIFFSAVNHLQLYSKKGTLVN
                     LYLMLLNLGIDILMRTVSLLVLNYFFDFRIINSYPTAWIYWVALFLAEDFMYYWLHRI
                     DHFCRLFWAVHVTHHSSEEFNLTVGFRSSVLQPLYRFIYFIPLSVVGFKAIDIMFIYS
                     ATQIFGILVHTQTVGKLGWLEYFLVTPSHHRVHHASNVCYLDRNMGMVLIIWDKLFGT
                     FQKELEHEPVKYGLTQNLQSNNPGKIVFHEWQDIICDLKKKTSFKNKIMYLLGPPGWS
                     HDGNRKTSAQLRKALLKKK"
     gene            complement(1836..3089)
                     /locus_tag="PKY09_08865"
     CDS             complement(1836..3089)
                     /locus_tag="PKY09_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006005223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNB13008.1"
                     /translation="MPSVCFYFQVHQPFRIKHYSVFDIGKDHFYEDGEKNKWIMDKVS
                     EKCYLPANKKMLELIKRHKGKFRISYSLSGTVIEQMELFRPDVLQSFVELAKTGCVEF
                     LSETYFHSLSFLYNKEEFERQVKEHDQKIEQYFKQKPTVFRNTELIYNNELAAYIEKL
                     GYKGILCEGVDRLLKEKHPNHLLKPTGTKSIKALLKNYRLSDDIAFRFSDKNWSEWPL
                     HADTFASWVHKVAGNGDVINLFMDYETFGEHQWESTGIFDFMDHLPREILKHPDFGFM
                     TPSQIVETYTPRGVYDAHEFISWADSERDLSAWLGNNMQQEALTKIYSISERVLGSGN
                     KDLIDVWGKLQTSDHFYYMSTKFWSDGDVHKYFSPYNSPYDAYIFYMNAFSDFESTLS
                     EESKPKKKAAAKKPAVPRNKKHAAE"
     gene            complement(3252..5060)
                     /gene="rpsA"
                     /locus_tag="PKY09_08870"
     CDS             complement(3252..5060)
                     /gene="rpsA"
                     /locus_tag="PKY09_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNB13009.1"
                     /translation="MSEVKHNIPPTDFDWDNVGKTADIYKSDDRQRMEAMYDQTLNTL
                     TEHEVKMGTIISISDKEVVVNIGFKSDGMVPLSEFRHMPDLKVGDEIEVYVETREDKN
                     GQLILSHKKARALKSWERVNESLEKDEIIQGYVKCRTKGGLIVDVFGIEAFLPGSQID
                     VKPIRDYDVFVGKTMEFKVVKINHEFKNVVVSHKVLIEEELESQKLEIMSKLEKGQVL
                     EGTVKNITSYGVFMDLGGVDGLLHITDISWGRINHPDEVLKLDDKINVVILDFDEDKK
                     RIALGLKQLTPQPWEALDQNIQVGDKIKGKVVVLTDYGAFIEVIPGVEGLIHVSEMSW
                     SQHLRSPQDFLKVGDEIETVILTLDREERKMSLGLKQLMPDPWVKIMEKYPVGSKHTA
                     KVRSLTNFGVFVELEEGIDGLIHISDLSWAKKIKHPAEFTKVGENIEVVVLEVDSEHR
                     RLSLGHKQLEENPWDTYETIFHVDTVHSGVVGKVTDKGATIIFPSFGVEGFAPYRHLM
                     KENGTPAKADETLDFKVIEFSKENKKIVVSHSRLFEEAKASEKMARDNEKAKDESDAF
                     KSVKKIKESQEKTTLGDLSVLSALKDEMKKNEAENN"
     gene            complement(5298..6308)
                     /locus_tag="PKY09_08875"
     CDS             complement(5298..6308)
                     /locus_tag="PKY09_08875"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNB13010.1"
                     /translation="MLKKILIGFVVLIGLLCVGSLFISNTYKIERDIYIKAPASVVFN
                     QINNLKNWSKWNTWLKMDTTMKVTYNNIEAGVGAQYQWTSSNKNVGNGTMQIKKSVQD
                     QLVESELTFEGEGNAVNGFNIQPAGDSVKLTTYFESEVGWNPLHKYLMIMFKGMMIDM
                     TDNGLKAIKELAENMPAQPGFEYNIQQVDITTTIPYLAIRCTSGLDSIGYFIGSSYGL
                     IDAEMKMQKLEMSKTVHPFAIYYKWENNLFEYDVCMPLAAKGKSAGNIVAGEVKTGKY
                     LMTQYFGNYDNSNKAHEAIVNYAKDNSIKISDAPLEFYITDPSVEKDTAKWQTDIYYQ
                     IN"
     gene            complement(7014..8507)
                     /locus_tag="PKY09_08880"
     CDS             complement(7014..8507)
                     /locus_tag="PKY09_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13011.1"
                     /translation="MSLRFLILYFLFFSMPLSVWSAPYLDYNERCKNAYELIFKLRFD
                     QALDSLDAEKREHPDNLIPGYLYHYHLFIKTFIGEEQATFNELKHNHEIITGQIKAIP
                     AVSPLRNFCLAECYLMTALSKLKFKEYLTAALQVKKAYGLLTENQKKFPEFELNLKSL
                     GVLHAFIGAVPENYQWLVKIAGMNGSVSNGIKELEQSFMMTREDSDAFHFMNKEIAFT
                     YIFTRNQLKPDPEWVIKFLSGLKLENEPLDLFFSTNIYYSTGRSAQIIPLLENYHADD
                     AVYPIHYFQFLLGMSKLDKLDYSSFSNFNNYVKLHKGNSFIKAAYQKMAWIKLLQNDI
                     DGYTALITEVKTVGNDFTDEDKQALNELVTHQKPNTFILKIRLLFDGGYYQEALKLLA
                     KGSSEVKTAKDQLEYKYRLARVLDKLDMTDRAKEFYLKTIEHGQDLKFYYAAASCNFL
                     AQIYEKEKNYALAETYYKKCLSLRDHEYQNSLDQKAKAGLSRIKDER"
     gene            8653..9804
                     /gene="nhaA"
                     /locus_tag="PKY09_08885"
     CDS             8653..9804
                     /gene="nhaA"
                     /locus_tag="PKY09_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNB13012.1"
                     /translation="MSGGKIINPIRKLAVQGKLAGLLIIVLTMLSLLLSNLSATSEYT
                     NLWHRHINLMGYDVSVLHMINDGLMVVFFLMVGMEIKRELYVGELANKRNAMLPVIAA
                     FGGMIVPGIIYAVFNIRDTHLLHGWAIPTATDIAFSLGVLSMLGNRVPFALKVFLTAL
                     AIIDDLGAIIIIALFYTEPENFHLSFLISSLLIVGALMFLSNKGFFNLFLWIIAGIVI
                     WYFMLLSGVHTTIAGVLIAFTIPLNKLSSFENKLQLPVNYFILPVFALANTAIVLSLD
                     NVSGLFSTVGLGVSLGLLIGKPLGITLASWVAVKNKWCLLPGKISLKLIFGAGLTAGI
                     GFTMSIFITSLSFKEPLLLDTSKIAIIAGSFLSGLAGVLFLKSNLKTKQ"
     gene            complement(9812..10576)
                     /locus_tag="PKY09_08890"
     CDS             complement(9812..10576)
                     /locus_tag="PKY09_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13013.1"
                     /translation="MKFFNLIFRRQYPVKRFLVFELIMFCMFSGNVFSQKGVATIGFQ
                     FKPIFNNDFFRTGSQSATQGTAEVTIQPNSGYCAGMVIRRGFTERLSLETGINYARRN
                     YQIQVTDSALSHKRNYKFIGYEIPISALVYIRLGQKMYIDASLGNSFDFYPSDIYIGD
                     DYFESEGLRKSWIKPAIIANVGAEYRTEKSGYFYLGVSFHRPYSFIYRSRTLISGRNY
                     KVFETDISGNYLTVDIRYFFHEDPLNKQRKRYPEDD"
     gene            complement(10590..11918)
                     /locus_tag="PKY09_08895"
     CDS             complement(10590..11918)
                     /locus_tag="PKY09_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13014.1"
                     /translation="MSLKLLNTFIILLIALQSNSQQVKFHQEWLDNPAKYSSEIEDVF
                     TGDTLRAFQKEEYKTFTILENGYAKSQIKNTADWKGIPSGFYADTIIFVYTQYPKNIN
                     FWLTNYHTLLAQRLKNLFKLSSDLNNEHTVYKILLQTDCDTDDEAKKMFHGIAIHLAP
                     IKSEFAKQQENIIKQQVYDDSLKNTKHSDQNTIATRKINSFIANNGGITDSVVYKIFS
                     RHPEWDNCLVIMDWTGSMYPYGAQAVLWHSLNLKQSGIKYFVFFNDGNMEKRKKIGKT
                     RGVYFEKAENINKIIGLLSKVKAKGNGGDPEENDVEAILKGIQKYPDFKNLILIADNN
                     SCMRDYCLTKYIKVPVKVVLCGTYSGINPQYINLAYKTKGSIHTIEDDISDFYNRTNT
                     DATFMIDGSEYRYDAKRDLFEYLTKPDKDDPHFCEPFYKKKKCKCENLNY"
     gene            complement(11922..12428)
                     /locus_tag="PKY09_08900"
     CDS             complement(11922..12428)
                     /locus_tag="PKY09_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNB13015.1"
                     /translation="MENRQVILNHDQIQQKLRRIACQIYEDNINEKSIYIAGIARSGY
                     VMAAKLRDIILERYPLKVIMLEVIVDKSNPLYPTIKGIDNTALLKNKVVIIVDDVLNS
                     GKTLIHSLRPFLEADAKKISTALLVDRDHKRYPVATDFVGLTLSTTNHEHIVADLKKG
                     KKDVVYIS"
     gene            12560..15427
                     /locus_tag="PKY09_08905"
     CDS             12560..15427
                     /locus_tag="PKY09_08905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HNB13016.1"
                     /translation="MALPQKRHLIKGAFFPFIILILTLSTTHAQKQFRFPAGITTDDY
                     EKGVLIVRIKSDYRSFCNESYINSVDLHQIFNNLSVTEVKKKFPNHKQPVESKTSFGF
                     PLTDLSLIYTLKFNAAIPVEKAASLLLSCKEVQYAEPSYIDKPLYTPNDTLATPQYQY
                     HHLNIHTFDGWDIQKGDTNIVIGITDTGIDTVHVELINQLKYNYADPINGIDDDGDGY
                     IDNYRGWNVAFNSNDVQGSVPLHGNFVAGISNAEVDNVTGIAGVGFKTKFLPVSCSPG
                     SNVIVNGYDAIVYAADHGCSVINCSWGGFGSSQFSQDVINYATFNKNALVVAAAGNAN
                     NDAPFFPASYQYVLSVGGTDSADVKWTSSPTSGSSFGSYVDVAAPGDKIFSIFPTNSN
                     PPYWYSGGTSEAAPQVSAIAALVKAQFPALNALQIGERIRATCDDIYSIPGNATFINK
                     LGKGRVNLLSALTQPATSVRAFDIQNTDNNDNAFSPNDTIRISAVFTNLLDPVNNLTV
                     TILSDNPDISFLNNSFLAGSMATMQSDSNRSNPFLAVIDPSIPKNTKVVFTLTFSSGA
                     YVDIQQFEVTVNVDYINVLVNDIGVTVTSKGRIGFNDSGNSQGIGFTQNEGPNLLYSG
                     SLMIGVNDSVVSDAVAGTPAGTINEHFTPLDYIHPIVPSVVSEFDLTTKFDDNASSNP
                     IGVNVVHNTYVWSTPADRKYVIAEYVIANNSNNTYSDLYAGIYADWDITENTYATNRA
                     MFDSTLKMGYAFEVTANNNFTGIKLLTPGTAHYYAYNNDGSDGSFNIYDGFSKQEKYQ
                     SMSGAGRAQAGMNGNGTDISMLLSSGPFSMLPFDSVKIAFALLGGDSLNEIVATAAAA
                     QVRYNTVGIGEVTNLENEIFVYPNPVASVVNVCVSNPITGFARIGLYDVYGHLVTTQS
                     YMLTTKSTIKIDVSTLSRGVYFLQLTTNQLNKTIKINIQND"
     gene            15420..15884
                     /locus_tag="PKY09_08910"
     CDS             15420..15884
                     /locus_tag="PKY09_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNB13017.1"
                     /translation="MINPEKLQFLKNNFIELLKKLKGNEVPVWGKMNAQQMIEHFGDA
                     IEDACGKNNRTLITPPDKLEATRAFMLSEKDFKPNTKNSLMSETPAATKLPDLASAIA
                     RVENGIRDFEAFYSGNTEKKVMNPFFGELNYEENIQLLHKHAMHHLRQFNLI"
     gene            15893..17041
                     /locus_tag="PKY09_08915"
     CDS             15893..17041
                     /locus_tag="PKY09_08915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB13018.1"
                     /translation="MSESRKLNSVIIVGPAYPFRGGIANLNETLCSHLNANGIVTEIV
                     SFTLQYPSVLFPGKTQLETSEQHFNFKITRLINSTNPLTWAKAVGYIKTRKPDLVVFR
                     YWLPFMAPCLGFIARKLRKHGFPVVAIADNIIPHEKRIGDQLLTKYFIQSCDAFIVMS
                     DKVAEDIKMFNPKKPVSITPHPIYSIFGDKVNRTEACDKLNLDNGYKYLLFFGFIRKY
                     KGLDILLQAMPLIKNKNLKLIVAGEFYESKEEYLNLIKAHHLENAIILRDEYIPKDDV
                     RYYFSVADLVVQPYRSATQSGVTQIAYSFDKPMVVTNVGGLPEMVTDGISGFVTDVNP
                     NAIANAVDRFFVEQLATSFTAGVQSEKNKFGWDKMIAAVKDIYQTLLK"
     gene            17082..>19128
                     /locus_tag="PKY09_08920"
     CDS             17082..>19128
                     /locus_tag="PKY09_08920"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNB13019.1"
                     /translation="MFLRRTFLICFLLLYHFVNAQTERCGTQVYLEQWLAENPQLRKT
                     YEHQQAAAASRIENSTAMTATITIPVVFHVVYNIAEQNIPDDRILEQLDVLNNDYNRL
                     NQDGQSTPSPFLGVSAATNIQFCLAKRDPNNKPTSGIIRKQTTVQSFSTGDEVKKTAT
                     GGSKGWSKSKYLNIWICNLTGNLLGYATMPGTANDTLDGVVVSYSTIGGPDHVGVHPT
                     YNLGRTLTHEVGHWLNLIHIWGDDGGTCTGTDNVADTPNQASEHFGTPNFPIISCSNN
                     PDGDMFMNFLDYTDDYVMNIFTAGQSLRMQDAVNNSRASLLTSRGCIDTTTAIDVALQ
                     NVINIETIYCQGIITPSAVIKNKGTATINSLTFSVKIDDNVAVNKAWTGSLVAGATTT
                     VALDPLTVPAGTHYLNVSVTQSGDGNVSNNISVKRITVTGATALPYSQDFETKRFPAD
                     NWLVLNEDNDATFMRSRLASINGSSSLWFYAPLQIPAGSKDIVQLEPFDLTTLAEPVL
                     AFDVACLHDNSISTTASLKILASTDCGSTFSQIFEKTGTALTTAEGSASDVFVPGAEQ
                     WRKDSVNLKALNSFNNVILRFEYTSGNANALFIDNLQIDRLGVIYPDASNVNVSVYGQ
                     STYGMLDFSVLLLKTVDFNIDIFDVAGRRVFSKSYSGSAVSDRLSLHQLNAGVYFFRV
                     "
     assembly_gap    19129..19228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19229..20001)
                     /gene="sucD"
                     /locus_tag="PKY09_08925"
     CDS             complement(<19229..20001)
                     /gene="sucD"
                     /locus_tag="PKY09_08925"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNB13020.1"
                     /translation="MSVLVNKNSKIIVQGFTGTEGTFHAGQMIEYGTNVVGGVTPGKG
                     GTTHLDRPVFNTVADAVKQTAADVSIIFVPPAFAADAIMEAAQAGIKLIVAITEGIPT
                     KDMIQVRDYLRDKEARLVGPNCPGVITAGECKVGIMPGFVFKKGRIGIVSKSGTLTYE
                     AADQVVKAGAGISTAIGIGGDPIIGTTTKEAVELFMNDPETDGIIMIGEIGGSLEAEA
                     ARWIKNNSKKPVVGFIAGQTAPPGRRMGHAGAIVGGADDT"
BASE COUNT         6259 a         3788 c         3640 g         6590 t
ORIGIN      
        1 agtttagaat ccttctttaa tttgaaaatc cttcaactgt gtttgcgtgt tccgttgccg
       61 ccttatgatg gcgcgagtgt gtcgctcgtt gcctttcatt ttaatggctt gcagccataa
      121 cattaatata tctttcgcca aaagtgaaaa taataaaatg acatgagcga cacgctcgcg
      181 cctactgagt atagaagcaa acagaagaat gcaattgaac cattagatga attaaaccaa
      241 aacaatttta ttttgtttat tcgtgacaga cttcaaaaat gtattacatg aaaggattaa
      301 tcgcattaat tatgtttgta ggttgtcaga atcatgcgca gccatctcaa gaaaattcta
      361 attttacaaa tatggaaacc aacatagcta cagacacagc tttatttggc acaggatgtt
      421 tttggtgtac agaagctgtt tttgaaacaa ttgatggcgt catctcagca acatcaggct
      481 attccggtgg tacagttgct aacccaagtt atcagcaggt ttgtatggga aacaccggac
      541 atgcagagtg tattcagata gtatttgatc caagcaaaat aacctatgat gaattgctgg
      601 aagtgttttg ggaaagtcat gacccaacta cattgaacag gcaaggtgct gatgtaggaa
      661 ctcaataccg ttctgttatc tttttttata atgacatgca gaagcaaact gcagagaact
      721 acaagaaaaa actgaatgag gaaaatgcat tcgggaaacc gatagtaact gaaattactg
      781 cagcgcaaca attttaccct gcagaaaatt accatcagga gtattttaaa ctgaatggac
      841 atgaaccata ctgtcagttt gtaatacagc ctaaggtaga aaagataagg aaaattttca
      901 gttcaaaaat caagcaataa atgaagctat ttctttttta atagagcttt gcgcagttgt
      961 gcacttgttt ttctattgcc atcatgactc catcccggag ggccaagcaa gtacatgatt
     1021 ttatttttaa aagacgtttt ctttttcaaa tcgcagatga tatcctgcca ttcgtgaaaa
     1081 acaattttgc cagggttatt agattgaagg ttttgtgtca gaccatactt aacaggttca
     1141 tgctccaatt ccttctggaa agtaccaaac aatttatccc aaataatcaa aaccattccc
     1201 atgtttctgt caagatagca aacattgctt gcatgatgta ctctgtgatg tgatggcgtt
     1261 accagaaaat attcgagcca ccctaattta cccacggttt gtgtatgaac caatattccg
     1321 aaaatttgcg ttgcgctgta tataaacatg atgtcaatag ccttaaagcc aacgacacta
     1381 agtggaataa aatagatgaa cctgtacagt ggttgcaaaa cagaagaacg gaaacctaca
     1441 gttaaattaa attcttcgct gctgtggtgt gttacatgaa cagcccaaaa caaccgacaa
     1501 aaatggtcta tccgatgcag ccagtaatac ataaaatctt ctgcaagaaa taaagctacc
     1561 cagtaaatcc atgcggtagg gtaggagtta ataatcctga aatcgaaaaa ataatttaga
     1621 actaacagac ttacagttcg cattaaaata tcaatcccca aattcaacag catgagatag
     1681 agattgacaa gagttccttt ttttgaatag agctgtaaat gattaacggc agaaaaaaat
     1741 atttcagcac caataacaac aaggtaaata ggtgttgaaa tcaaaatcag cagcgactct
     1801 ctgaaatgct ccatttaaat tactgaagta aaactttatt cagcagcatg cttcttatta
     1861 cgtggcacag caggcttttt tgcggctgct ttcttttttg gtttactttc ttctgataat
     1921 gttgattcaa agtctgaaaa tgcattcatg taaaatatgt aagcatcgta tggtgagtta
     1981 taggggctga aatatttatg tacatcgcca tcgctccaaa acttagtact catataataa
     2041 aaatgatcgc tggtttgcag tttgccccat acgtcaatta aatctttatt gcccgatcca
     2101 agcactcttt cgcttataga ataaattttt gtgagagctt cctgctgcat gttgtttccc
     2161 agccatgctg aaagatcacg ctcgctatca gcccaactga taaactcatg tgcatcatat
     2221 acaccacgag gggtgtaggt ttcaactatc tgagatggtg tcataaatcc aaagtcagga
     2281 tgttttaata tttcacgtgg cagatgatcc ataaaatcaa aaatgcctgt gctttcccac
     2341 tgatgttcgc caaaagtttc atagtccata aacaggttta tcacatcacc attcccggct
     2401 actttatgta cccaacttgc aaatgtatct gcatgcaatg gccattcgct ccagtttttg
     2461 tcggagaatc taaaagcaat gtcgtcactt aaacgataat ttttcagcaa tgctttaatt
     2521 gattttgtac ctgttggttt caataaatga ttaggatgtt tttctttcaa taaacggtca
     2581 acaccttcgc acaatattcc tttatagcct aacttttcga tataagctgc aagttcatta
     2641 ttgtaaatta attcggtatt tctgaaaaca gtaggcttct gtttaaagta ctgttcaatt
     2701 ttctgatcat gttcttttac ttgtctctca aattcttctt tgttatataa aaaactgagc
     2761 gaatgaaaat aagtttcaga gaggaattca acacaacctg ttttagcaag ttcaacaaag
     2821 gactgcaaca catccggtct gaacagttcc atctgttcaa tcactgtacc gcttagtgaa
     2881 taacttattc tgaatttgcc tttatggcgc ttaatcagct caagcatttt cttgtttgca
     2941 ggcaggtagc atttctcaga taccttatcc ataatccatt tatttttttc gccatcttca
     3001 taaaaatgat ctttaccaat gtcgaaaaca ctgtaatgtt ttattctgaa gggctgatgc
     3061 acctgaaagt aaaagcaaac cgatggcata aatttgtttt tataaatggt cgttgtgcca
     3121 aaattaaact tttgcctgaa acaaaagaaa attagctaaa agtgtcatca taaatttatg
     3181 tttgtagtag ggtacaaaaa tcaaaaagcc gcttttaagc ggctttctga ttttattgat
     3241 gtcaatgcag attagttatt ttctgcttcg ttcttcttca tttcatcttt aagtgcagaa
     3301 agtacgctca aatcacccaa ggttgttttt tcctgtgatt ctttgatttt cttaacagac
     3361 ttaaatgcat cactttcatc tttagctttt tcgttatcac gagccatctt ttcagatgct
     3421 tttgcttctt caaacaaacg gctgtgcgat acaacaatct tcttgttctc tttgctgaat
     3481 tcgattactt taaaatcaag tgtttcatca gctttagcag gagtgccgtt ttctttcatc
     3541 agatgacgat atggagcaaa accttctact ccaaatgatg ggaaaatgat ggttgcacct
     3601 ttatcggtta cttttccaac aacaccggaa tgaacagtgt caacatggaa aattgtttca
     3661 taagtatccc atggattttc ttctaattgc ttatgtccaa ggcttaatct gcgatgctct
     3721 gaatcaactt caagaactac aacttctatg ttttcgccaa ccttggtaaa ctcagcagga
     3781 tgtttaattt tcttagccca tgaaaggtca gagatatgaa ttaaaccatc aataccttct
     3841 tccaactcaa caaaaacacc aaagtttgtc aggctgcgaa cttttgcagt atgctttgag
     3901 ccaactggat atttctccat gattttaacc caaggatcag gcatcagttg ctttaatcca
     3961 agtgacattt tgcgctcttc gcgatcaaga gtaaggatta cagtttcaat ttcatcacca
     4021 actttgagga aatcttgtgg gctgcgtaaa tgttgcgacc atgacatttc tgatacgtgt
     4081 atcaaaccct caacgcctgg aattacttca atgaaagcac catagtcagt caacacaaca
     4141 actttgcctt tgattttgtc accaacttga atattttggt caagtgcttc ccatggctga
     4201 ggcgtaagtt gtttcaagcc aagtgcaata cgttttttat cctcgtcaaa gtcaaggatt
     4261 acaacgttaa ttttatcatc taacttcaac acttcatcag gatggttgat tcgtccccat
     4321 gaaatatcgg taatatgcaa taagccgtca acgccaccaa ggtccataaa tacaccgtaa
     4381 gatgtgatgt ttttaacagt accttcaaga acctgtcctt tttcaagttt tgacatgatt
     4441 tctaactttt gactctcaag ttcctcttca ataagtactt tgtgagatac aactacgttc
     4501 ttaaactcat ggttgatttt aaccacctta aattccatag tttttcctac aaacacatca
     4561 tagtcacgga ttggtttcac atcaatttga gaacccggta aaaatgcttc aatgccaaac
     4621 acgtcaacga taagtccgcc tttggtacgg catttaacat aaccctgaat aatttcatct
     4681 ttttcgagag attcattaac acgctcccag gatttaagag cacgagcttt tttatgtgac
     4741 aatatcaatt gcccgttttt atcttcacgg gtttctacat acacttcaat ttcatcccca
     4801 actttcaggt cgggcatgtg gcggaattcg ctcagcggaa ccattccatc tgatttgaat
     4861 ccaatgttta ccaccacttc tttatcagaa atgctgatga ttgtacccat tttcacttca
     4921 tgttctgtaa gtgtgttcag ggtttgatcg tacatggcct ccatacgctg gcggtcatct
     4981 gatttgtaaa tgtcggctgt tttaccaacg ttatcccaat cgaaatctgt cggtggaatg
     5041 ttgtgtttta cttcactcat taattgtttt gtttttttaa aaattgaaat tgttagactt
     5101 tatttattat ttatgaatag acctattaaa acgggcgcgc aaagataggt atttgctttg
     5161 ataaataaaa ctaatatgct tgataattat tcagttccgt ttgttgaaga tgagttagcc
     5221 ggaaatggcc aaaaagtcaa taaaaaaggg ctaaacttgt ctgcttaacc ctaaatcttt
     5281 atgattttaa tacaaaatca attaatctga tagtaaatat ctgtctgcca tttggcagtg
     5341 tctttctcaa cagaagggtc ggtaatataa aactcaagag gtgcatcaga gattttaata
     5401 ctgttatctt tagcgtagtt aacgattgct tcatgagctt tgttgctatt atcataattg
     5461 ccaaaatatt gagtcataag atatttccct gttttgactt cgccagcaac aatatttcct
     5521 gctgattttc cttttgctgc caagggcata caaacatcat attcaaatag attattttcc
     5581 cacttgtaat atatggcaaa tggatgaacg gttttactca tttccaattt ctgcattttc
     5641 atttctgcat ctatcaagcc ataacttgac ccgataaaat agcctatgga gtccaaaccg
     5701 gatgtacatc tgatagctaa atatggaata gttgtagtga tatctacttg ctgaatgttg
     5761 tactcaaatc cgggctgtgc gggcatgttt tctgctaatt cttttatagc tttcaatcca
     5821 ttgtcagtca tatcaatcat cataccttta aacattatca tcaggtattt gtgtaatgga
     5881 ttccaaccga cttcactttc aaaataggtg gtaagcttaa cactgtcacc ggcaggctga
     5941 atattaaagc cattaacagc atttccttca ccttcaaaag tcagctcaga ttctactaac
     6001 tgatcttgca cacttttttt aatctgcata gtgccattcc caacattctt attgctggat
     6061 gtccactgat attgtgctcc aacaccggct tcaatattgt tatacgtaac tttcattgtt
     6121 gtatccattt taagccaggt attccatttg ctccagttct tcaggttgtt aatctgatta
     6181 aacacgacag aagcaggagc ttttatataa atatcgcgtt caatcttata ggtgtttgag
     6241 atgaaaaggg agccaacaca taacagcccg atgaggacga caaatccaat tagaattttc
     6301 tttagcataa caatttgatt tagtagtttt acgaaggtaa aagtagcatt aatgactatg
     6361 caaaagcatt tcgaatacaa taaaaatgga aaatgatatt aataggagga attgccttta
     6421 gtattctcta aatgcaatat attatgttaa atatgaatta ttgaattagt ttcaatagct
     6481 gttgcgaaat cataagcttt attaaaattg tcaattaaaa gttcttttaa cttaaaacaa
     6541 gttctttatg taatatccgg aacaattcat ttgaatgttc tatacattta ataattaatt
     6601 aaatatgatt aattaaatca aaataacttt aagtatattt tattattgac ttgataatta
     6661 aaataataca atttagtatt ggaaaatgcc tgtttaaatt catcacgata aaattcattt
     6721 aaccaatagg ggactttaaa gtgatttgcc ataaactgat ttaataaact ttagaaataa
     6781 tcagattaaa tagatacttc cagcttagtt tttatacaac tttttctgag agaaattaaa
     6841 actcaaacaa taatatggtg tatgaaaaac gatgcaaaag catcagattg gaattttata
     6901 aaatgcaggt caagtgatgt aaaagcaaca tacttattag agaatcctat cccgctatcc
     6961 tcaatatttc aatcttataa ttaatattat gttaaatagt aattgtttga ttgttacctc
     7021 tcatctttaa ttctgcttaa cccggctttt gccttttggt ctaaactgtt ctggtattca
     7081 tggtcgcgta aactaagaca ctttttataa tacgtttcag ccaaagcata attcttctct
     7141 ttctcataaa tctgcgccaa aaaattgcag ctggcagcag cataataaaa ttttaaatcc
     7201 tgcccgtgtt caatagtttt cagataaaat tctttggccc gatctgtcat atccagtttg
     7261 tccaacaccc ttgccaacct atatttatat tcaagctgat ctttagctgt tttaacttcg
     7321 cttgaccctt tagctaacaa ttttaaagcc tcctgatagt agccgccatc aaacaagagt
     7381 ctgattttta agataaaagt attgggtttc tgatgtgtaa ctaattcatt tagtgcttgt
     7441 ttatcttcgt cagtaaagtc gttacccact gtcttaactt cagtaattag tgctgtatat
     7501 ccgtctatgt cattttgcaa tagttttatc caggccattt tctgataagc agcttttatg
     7561 aagctattgc ccttatgcaa tttaacataa ttattgaaat tagagaatga tgaataatcc
     7621 aacttatcaa gtttagacat tcctaaaaga aattgaaaat aatgtattgg ataaaccgca
     7681 tcatctgcat gataattttc aagcagtgga ataatctgtg cactgcgccc ggttgaataa
     7741 taaatgttgg tagaaaagaa caagtccaaa ggctcgttct ccaatttcaa ccctgacaaa
     7801 aacttgatta cccactccgg gtcaggcttt aattgattac gtgtgaaaat ataggtaaag
     7861 gcaatttctt tattcataaa atgaaaagca tcactgtctt ctctagtcat cataaaagat
     7921 tgctccaact cttttatacc attcgataca ctaccattca ttccggcaat ttttactaac
     7981 cattgatagt tttctggcac tgcaccaata aatgcatgta gcacaccaag actctttaga
     8041 ttcaactcaa attcaggaaa ttttttttga ttctccgtaa gtaaaccgta tgctttcttt
     8101 acttgtaaag ctgcagtcag atactcttta aatttcagtt tcgacaatgc tgtcatcagg
     8161 taacattctg caagacaaaa attacgtaat ggtgataccg caggtatggc tttgatttgt
     8221 ccggtaataa tttcatggtt atgtttcagc tcgttaaaag tagcctgctc ctcccctatg
     8281 aatgttttga tgaataaatg atagtgataa agatatccgg gaatcagatt gtccggatgt
     8341 tctcttttct ctgcatcaag actatctaat gcctgatcga agcgcagctt aaaaatcagt
     8401 tcataggcat tcttgcatct ttcattataa tcaagatagg gtgctgacca tacacttaac
     8461 ggcatactga aaaacaaaaa atataaaatc aaaaacctaa ggctcatcag tctaataaaa
     8521 ctatcatgtt ttgcaataat acaaagacaa aatgacaaac tgaatcctct ttataaagtt
     8581 gtatgtgatt gtaaacattc gaaaacattt gtggtcgtat aaaaaaaata tatataattt
     8641 taattttgca aaatgtctgg aggtaaaatc ataaacccaa tacgtaagct tgcagttcaa
     8701 ggcaaattag ccggtctttt gattattgta cttacaatgc tatcgctgct gttaagtaat
     8761 ttatcagcaa catcagaata tacaaacctt tggcaccgcc acataaattt aatgggttac
     8821 gatgtaagtg tgttgcatat gattaatgac ggattaatgg ttgtgttttt tctgatggtt
     8881 ggcatggaaa taaaacgtga gctgtatgtt ggtgaactgg ccaacaaacg caatgcaatg
     8941 ttaccggtaa ttgcagcttt tggtggaatg attgttccgg gaattatcta tgcagtgttt
     9001 aatatcagag atactcacct gctgcatggc tgggcaatac ccacggcaac agatattgca
     9061 ttttctcttg gcgtactttc aatgcttggt aacagagtcc cgtttgctct taaagttttt
     9121 ttaactgcat tagcaattat tgatgacctc ggtgcaatta tcattattgc attattctac
     9181 accgagccgg aaaactttca tctttcattt ttaatcagca gtttactcat tgttggtgca
     9241 ttgatgtttc tttcaaacaa aggttttttt aatttatttc tgtggattat tgcaggcatt
     9301 gtcatttggt attttatgct gctttcaggc gtgcatacaa ctattgctgg tgtactgata
     9361 gcttttacaa ttccattaaa caaactttca tcattcgaaa ataaacttca gctaccggtg
     9421 aattatttta ttcttcctgt ttttgcattg gctaatactg ctattgttct ttctcttgat
     9481 aatgtttcag gattgttttc tactgtaggt ttaggggtga gtttaggttt gcttatagga
     9541 aagccattag gcataacttt agcatcatgg gttgcagtaa aaaacaaatg gtgtttactc
     9601 cccggaaaaa ttagtttaaa gttgattttt ggtgcaggtc taacagccgg tataggtttt
     9661 acaatgtcaa ttttcattac ttcactttca tttaaagaac ctctgcttct cgacacttca
     9721 aaaattgcaa ttattgcagg ctctttttta tcgggtttgg ctggtgtctt atttttgaag
     9781 tccaatctta aaacaaaaca atgatttaat attaatcatc ttcgggatat cgtttgcgtt
     9841 gtttgttcaa agggtcttcg tgaaaaaaat aacgaatatc aacagtcaga taattcccgg
     9901 aaatatcagt ttcaaatact ttataatttc tacctgatat taaagttctt gagcgataaa
     9961 taaaactata cggacgatga aatgaaactc caagataaaa ataacctgat ttttccgttc
    10021 tatattctgc tcccacatta gcgatgatgg caggtttaat ccaacttttt ctcaggcctt
    10081 cactttcaaa ataatcatca ccaatgtaaa tgtctgaagg ataaaaatcg aatgaattgc
    10141 ctaaagaagc atcaatatac attttctgtc caagcctaat atataccaat gcactaatcg
    10201 gtatttcata tcctataaat ttataattac gtttatggct caatgctgag tcagtaactt
    10261 gaatctgata atttcttctt gcataattta ttcctgtttc aagagaaagt cgctctgtaa
    10321 aaccacgtct gataaccatg cctgcacaat atcctgaatt gggttgaatg gttacctcag
    10381 cagttccttg tgtggcagat tgtgagccag tacgaaaaaa gtcattatta aaaatcggtt
    10441 taaactgaaa gccgattgtt gctacacctt tttgcgaaaa cacgttgccg gaaaacatac
    10501 aaaacatgat tagttcaaag actaaaaagc gttttacagg gtattgtcgc ctgaaaatta
    10561 ggttgaaaaa tttcattcct gaaaataaat taataattta aattctcgca tttacacttc
    10621 ttctttttat aaaaaggttc gcaaaaatgt gggtcatctt tatcgggttt cgtcagatat
    10681 tcaaataaat ctctttttgc atcataacga tattctgaac catcaatcat aaacgttgca
    10741 tcggtattgg ttctgttgta aaaatcactg atgtcatctt caatagtatg aatgctgcct
    10801 ttggttttat aggccaagtt tatatactgc ggattgatgc cactataggt tccacagagg
    10861 acaaccttaa ccggaacttt gatatatttt gttaaacaat aatcgcgcat acaggaattg
    10921 ttatcggcaa ttaaaatcag gttcttaaaa tcaggatact tctgaatgcc tttcaatatt
    10981 gcttctacat cattttcttc ggggtcgcca ccattacctt tggctttaac cttactcaac
    11041 aagccaatta ttttatttat gttttctgct ttttcaaaat aaacacctct ggtctttcca
    11101 atttttttcc gtttttccat attaccatca ttaaagaaaa cgaaatattt tatacctgat
    11161 tgtttaagat tcaacgaatg ccaaagtaca gcttgtgcac catagggata catactgcct
    11221 gtccaatcca ttatcaccag gcaattgtcc cattccggat gacgcgaaaa aattttatac
    11281 accacactat cggtaatacc gccattgttg gcaataaagc tgtttatctt tctggtggca
    11341 attgtattct gatctgagtg tttggtgttt tttaaagaat cgtcatacac ttgttgtttg
    11401 ataatatttt cctgctgctt tgcaaattcg ctttttattg gtgctaaatg aatggctata
    11461 ccatgaaaca ttttttttgc ttcatcatca gtatcgcagt cagtttgaag cagtatttta
    11521 tatacagtat gttcattatt gagatctgag gaaagcttaa aaagattttt caagcgctgt
    11581 gctaatagcg tgtgatagtt tgtcagccaa aaattgatat tcttaggata ttgtgtataa
    11641 acaaaaatga tggtgtcagc ataaaatccg gaaggaatac ctttccagtc ggcagtgttt
    11701 tttatttgtg atttagcata accattttcc aaaattgtga atgttttata ttcctctttt
    11761 tgaaatgcgc gcaaggtgtc accggtaaaa acatcttcaa tttcagaact gtatttggca
    11821 ggattgtcga gccactcctg atggaatttc acttgctgcg aattactttg taacgctata
    11881 agcaatatta taaaagtatt cagaagtttc aacgacatga tttatgaaat ataaactaca
    11941 tcttttttcc ccttcttcaa atcggcaaca atatgctcgt gattggttgt agaaagcgta
    12001 agtccaacaa aatctgttgc cacaggatag cgcttatgat ctcggtcaac gagtaatgcc
    12061 gtacttattt ttttagcatc agcttccagg aaaggtcgca gactatggat aagtgtcttg
    12121 cctgaattta gtacatcatc aacaataatg accactttat ttttaagtaa tgcagtattg
    12181 tcaatccctt taattgttgg atacaatggg tttgatttat caacaatcac ctccagcata
    12241 attactttaa gcggataacg ttccaaaata atatctctta acttggccgc cataacgtag
    12301 ccgcttcttg caattccagc tatataaata cttttttcat tgatattatc ttcataaatc
    12361 tgacaagcaa tacgtcttag tttttgctgt atttggtcat gatttaaaat tacctgacgg
    12421 ttttccattt tttcaattca cttaaagcgg cataaaaata gaatatttct attattgcct
    12481 tgatttttta agctttattg tactaattct tcgtttattt atacctaatt ttaccataat
    12541 atttaaaaaa cacttcagaa tggcactccc acaaaaaagg catttaataa aaggtgcatt
    12601 ttttcctttc atcatactga tattaacttt gtcaaccaca catgctcaaa agcagtttcg
    12661 ttttcctgcc ggcataacta ctgacgatta tgagaagggt gttttgatag taagaataaa
    12721 atctgattac aggagctttt gtaacgaaag ctatataaac tccgttgatt tgcatcaaat
    12781 ttttaataac ctttcggtga cagaagttaa gaagaaattt ccaaatcaca aacagcctgt
    12841 tgaaagtaaa acatcttttg gctttccatt gactgatttg tcattgattt atactttgaa
    12901 atttaatgct gctattcctg tagaaaaagc tgcttcattg cttttatctt gcaaagaagt
    12961 acagtatgcc gaaccatctt atattgacaa accgctttac acacctaacg atactttagc
    13021 aacgccacaa tatcaatatc atcatttaaa catacatact tttgatggtt gggatattca
    13081 gaaaggtgat accaatattg taatcggaat tactgataca ggcattgaca ctgttcatgt
    13141 tgaactgatt aatcagttaa aatacaacta tgctgaccct atcaatggta ttgacgatga
    13201 tggcgatggc tacattgaca attatcgtgg gtggaatgtg gcatttaact ctaatgatgt
    13261 tcagggctcg gttccgttgc atggcaattt tgttgcagga atatcaaatg cagaagttga
    13321 caacgtaaca ggcattgccg gagtaggttt taaaactaaa tttcttcctg tcagttgttc
    13381 tcccggttcg aatgttattg taaacggata cgatgctata gtatatgctg ccgaccatgg
    13441 ttgttctgtt atcaattgtt cgtggggtgg ctttggcagc tcacagtttt cgcaagatgt
    13501 cattaactat gctacattta ataaaaatgc tttagtagtt gcagcagcag gaaatgccaa
    13561 taacgatgcg ccattttttc ctgcatcata tcagtatgta ttgagtgttg gaggaacaga
    13621 ttctgcagat gtaaaatgga catcttcgcc aacatcagga agcagctttg gcagctatgt
    13681 tgatgtagca gcccctggtg ataaaatatt ttcgattttc ccaacaaaca gcaatccacc
    13741 ctactggtat tcgggaggta cttcggaagc tgcaccacaa gtttcagcaa ttgctgcctt
    13801 ggtaaaagca caatttcctg ctttaaatgc tttgcagatt ggcgaaagaa ttcgtgcaac
    13861 atgtgatgat atttattcca ttccgggcaa tgctactttt atcaataaac ttggaaaagg
    13921 aagagtgaat ctactgagtg cgctaaccca gcctgcaaca tcggtcagag catttgacat
    13981 acagaacact gataataatg acaatgcatt ttctcctaac gacactatca gaattagtgc
    14041 tgtatttaca aacctgcttg accctgtaaa taatttaaca gtaaccatcc tgtcagacaa
    14101 tccggacatc tcttttttga ataattcttt tcttgcagga agtatggcaa caatgcagtc
    14161 tgacagcaat cgcagcaatc catttttggc agtgattgat ccatcaatac ccaagaacac
    14221 aaaagttgtt ttcactttaa ctttttcatc aggtgcttat gtggacatcc aacaatttga
    14281 ggtaacggtg aatgttgatt acatcaatgt tttggtgaat gatattggtg taaccgttac
    14341 aagcaaaggc agaataggct ttaatgattc cggaaactca caaggaattg gctttacaca
    14401 aaacgaagga cctaacttac tctattcagg aagtttaatg attggtgtta atgacagtgt
    14461 ggtgagtgat gctgttgcag gtacaccggc aggaactatt aacgagcatt ttactccgtt
    14521 agattatatt catcccattg ttccttctgt ggtttcggaa tttgatttaa caacaaagtt
    14581 tgatgataat gcaagtagca atcctattgg tgtaaatgta gttcacaaca cctatgtatg
    14641 gagtacacct gccgatcgca agtatgtaat tgcagagtat gtaattgcca acaacagtaa
    14701 caatacttac agcgatttgt atgcaggtat ctatgccgac tgggacatta ccgaaaatac
    14761 ttatgcaaca aatagagcaa tgtttgattc tactttaaaa atgggatatg catttgaagt
    14821 tactgccaac aacaatttta caggaataaa attactcact cccggaacag cacattatta
    14881 tgcatataac aatgatggca gtgatggaag ttttaatatt tatgatggtt ttagtaaaca
    14941 agagaaatac cagagcatga gtggcgcagg tcgcgcacaa gcaggtatga atggtaatgg
    15001 cacagacatt agtatgttgt tgagcagtgg accattttcg atgttgcctt ttgactctgt
    15061 aaaaattgcc tttgcattac ttggtggcga cagccttaat gagatagtag ctacagcagc
    15121 agctgcacaa gtaaggtata acacagttgg cattggcgaa gtaacaaatc tggaaaatga
    15181 aatatttgta tatccaaatc ctgttgcatc tgttgtgaat gtttgtgttt caaatcctat
    15241 taccggattt gcaaggattg gtttatatga tgtatatgga catcttgtga ctacccaatc
    15301 ttatatgcta acaacaaaaa gtactataaa gattgatgta agtacactgt caagaggagt
    15361 ttactttttg caattgacga ctaatcagtt aaacaaaaca attaaaataa acattcaaaa
    15421 tgattaaccc tgaaaaatta caattcctga aaaataactt tattgaactt ctgaaaaagc
    15481 taaaaggcaa tgaagttcct gtttggggca aaatgaatgc acaacaaatg atagaacatt
    15541 ttggtgatgc cattgaagat gcctgcggaa aaaacaaccg cacattaata acgccacctg
    15601 ataaacttga agctacacgt gcatttatgc ttagcgagaa agattttaag cccaacacaa
    15661 aaaactcttt gatgagtgaa acacctgctg caactaaatt gccggatttg gcttcggcaa
    15721 ttgcccgtgt agagaatggt attagagatt ttgaagcttt ttattcaggc aatacagaga
    15781 agaaagttat gaatcctttt tttggtgagt tgaattatga agaaaacatt caactgttgc
    15841 ataaacatgc tatgcatcac ctgagacaat ttaatctgat ttaacaattg atatgagtga
    15901 aagccgtaag ttaaattcgg taataattgt tggacctgcc tatccttttc gtggtggaat
    15961 agccaatctc aacgagacac tgtgttcgca tttgaatgca aatggaattg tgaccgagat
    16021 tgtttctttc acactgcaat atccttcagt gcttttcccc ggaaaaacgc aattggaaac
    16081 atcggaacaa catttcaatt tcaagataac acgattgatt aattcaacta atccgttaac
    16141 gtgggcaaag gctgttggct atattaagac tagaaaaccg gacttggtag tgtttcgata
    16201 ctggctgcca tttatggctc cctgtcttgg ttttattgca agaaaactac ggaagcatgg
    16261 ctttcccgtt gtagctattg ctgacaatat tattccacat gaaaaaagaa taggtgatca
    16321 gttattgaca aaatatttta ttcagtcgtg tgatgctttt attgttatgt cagataaggt
    16381 tgctgaagat ataaaaatgt ttaatcctaa gaaaccggta agcattacac cacatcctat
    16441 ttactctatc tttggtgata aagttaaccg cactgaagca tgtgataaat tgaatcttga
    16501 taatggatat aaatatttac tttttttcgg gtttatcaga aaatataaag gattggatat
    16561 tctgttgcag gcaatgccct tgataaaaaa caaaaacctt aaacttattg ttgccggaga
    16621 gttttatgaa tcgaaagagg aataccttaa tctcataaaa gcgcatcatc tggaaaatgc
    16681 aatcatcctg agagatgaat atattccaaa agatgatgtg cgttattatt tcagtgttgc
    16741 tgaccttgtt gttcagcctt acaggtcggc tacacaaagt ggtgttacac aaattgcata
    16801 cagtttcgat aaaccaatgg ttgtaaccaa tgttggcggg ctgcctgaaa tggttactga
    16861 tggtatttcg gggtttgtga ctgacgtaaa tcccaatgct atcgctaatg ccgtagatcg
    16921 cttttttgta gagcaactgg caacatcatt taccgctggt gtacaatcag aaaagaataa
    16981 attcggatgg gataaaatga ttgctgccgt taaggatatt tatcaaactc ttttaaaata
    17041 aaaatattcg tttaaatttg tcatactaaa ttttctgttc aatgttttta agaagaactt
    17101 ttttaatctg ttttttactc ctgtatcact tcgttaatgc gcaaaccgaa cgttgcggca
    17161 cacaagttta tctggaacaa tggcttgcag aaaacccaca attgagaaaa acgtatgagc
    17221 atcagcaggc agcggctgca tcaagaattg aaaacagcac agcaatgact gcaacgatta
    17281 ctattcccgt tgtttttcat gtggtttata atattgcaga gcaaaatatt cctgacgaca
    17341 gaattttaga acaacttgat gtattaaaca acgattacaa tcgcctcaat caggatggac
    17401 aaagtacacc atcaccattt ctgggagttt ctgccgctac caacattcag ttttgtttgg
    17461 caaaacgcga tccaaacaac aaacctacat cgggcatcat aagaaaacaa acaaccgttc
    17521 agtcgttcag tacgggtgat gaagtaaaaa aaactgcaac aggtggctct aaaggatggt
    17581 cgaaaagcaa atatctcaat atctggattt gtaatctaac aggaaactta cttggctatg
    17641 caacaatgcc gggtacagca aacgatacgc ttgatggtgt tgtagtaagt tattcaacca
    17701 ttggtggccc tgaccatgtt ggtgtgcatc ctacctataa tttaggtcgt acgctgacac
    17761 atgaagtcgg gcattggctg aatctgattc atatctgggg tgatgatggc ggcacatgca
    17821 caggaacaga taatgttgca gacacaccca atcaggcatc ggagcatttt ggcacaccta
    17881 attttccaat catcagttgc agcaacaatc ctgatggcga tatgtttatg aactttcttg
    17941 attacactga cgactatgtc atgaatattt ttacagcagg tcagtcgttg cgcatgcagg
    18001 atgccgtaaa taacagcagg gcttcattgc ttacctcaag aggatgtata gacacaacta
    18061 ctgcaataga cgttgccttg caaaatgtta ttaatattga aactatttat tgtcaaggca
    18121 tcataacgcc ttctgcggtg attaaaaata aaggtactgc tacaataaac tcacttacat
    18181 tttctgttaa gatcgatgat aatgttgcag taaacaaagc atggacaggc agcttggttg
    18241 ccggtgcaac aacaacagtt gcattagatc ctttaacagt tccggcagga acacactatc
    18301 tgaatgtgag tgtgacacag agtggcgatg gcaatgtatc aaacaacatt agtgtaaaaa
    18361 gaattaccgt gaccggtgca acagccctgc cctatagtca ggattttgaa acaaaaagat
    18421 ttcctgccga caattggttg gtgttgaatg aggacaatga tgcaactttt atgcgcagta
    18481 ggctggcttc aattaatggc agctcctcgc tttggtttta tgcaccatta caaataccgg
    18541 caggtagcaa agatattgtg caattagagc cttttgacct tacaacgctt gcagagcctg
    18601 tgcttgcatt tgatgtagcc tgcttgcatg acaacagtat tagtacaacg gcaagtttaa
    18661 agatattggc atcaacagat tgtggcagta cgtttagtca gatttttgag aagactggaa
    18721 cagcactgac tacagctgaa ggcagtgcat cagatgtatt tgttccgggt gctgagcagt
    18781 ggcgaaaaga ctctgtaaat ctgaaagcac tgaatagttt taataacgtg attttacgct
    18841 ttgaatatac ttcgggtaat gccaatgcac tttttataga caaccttcaa attgaccgtt
    18901 tgggggtaat ttatcctgat gcatcaaatg ttaatgtgag tgtctatgga caaagcacat
    18961 acggtatgct cgactttagt gtgctgcttt taaaaactgt agattttaat atagatattt
    19021 ttgatgttgc aggaagaaga gtattttcaa aatcatatag cggcagtgct gtttctgacc
    19081 gattgagttt gcatcagctt aatgcagggg tttatttttt cagagttann nnnnnnnnnn
    19141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt atcgtcagca ccaccaacaa tagctcctgc
    19261 atgtcccatt ctgcggccgg gaggggctgt ttgtccggca atgaagccaa ctacaggttt
    19321 tttactgttg ttttttatcc aacgtgcagc ttctgcctcg aggctacctc ctatttcacc
    19381 gatcatgatg atgccatcag tttcggggtc gttcataaat aactcaactg cttcttttgt
    19441 agtggtacca ataatggggt cgccaccaat accaatggca gtagaaatac ctgcacctgc
    19501 ttttacaacc tgatcggcag cttcgtaggt tagtgttccc gatttagaaa caatgccaat
    19561 gcgacctttc ttaaatacaa aaccgggcat gatgccaacc ttacattcac cggcagtgat
    19621 tacaccggga cagttggggc ccacaagtcg tgcttctttg tcgcgcagat agtcacgcac
    19681 ctgaatcata tctttagtag ggatgccttc tgtgatggca acaatgagct tgatgcctgc
    19741 ctgtgcagct tccataatag catctgcagc aaaagcagga ggaacaaaaa tgatgcttac
    19801 atcggcagcg gtttgcttta cagcatcggc aacggtatta aaaacaggtc tgtccaaatg
    19861 cgttgttccg cctttgccgg gggttacacc accgacaaca ttggtgccat actcaatcat
    19921 ttgtcctgca tgaaaagtgc cttctgttcc ggtgaaaccc tgaacaataa ttttagaatt
    19981 cttattgact aaaacgctca ttgcttaatt ttgtagtggt gagttgttta ttttttaaaa
    20041 tgtggtgcga aggtaaggaa attgtatgtc atgagtcaat taccaaaaag tcttttttac
    20101 attaaatttc cgccatctct aaaataattc ctatctttat tgctaaaatt ataattgtta
    20161 aattgggtgc ttcgcggcta ccttaggcta aatgactaag tgccgcgcaa tttttttata
    20221 cccaaccgat acaattctat gatgagagca agatcaagtg tatattatac caacaacgga
    20281 caaccgatgg cgcccttctc tgcttacgat acactgacag cgccacagct tacaggctta
    20341 gtgcagatac ccattgactc actgcccgtc atgcatt
//
LOCUS       DAOQNP010000060        19687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_957_length_19687_cov_8.578087, whole genome shotgun sequence.
ACCESSION   DAOQNP010000060 DAOQNP010000000
VERSION     DAOQNP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19687
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_957_length_19687_cov_8.578087"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="PKY09_08930"
     CDS             <1..99
                     /locus_tag="PKY09_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNB13021.1"
                     /translation="LRTHLKRLNRRTICFTRSVIMLSACLKIYFWG"
     gene            complement(119..7450)
                     /gene="sprA"
                     /locus_tag="PKY09_08935"
     CDS             complement(119..7450)
                     /gene="sprA"
                     /locus_tag="PKY09_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNB13022.1"
                     /translation="MQGLKLLRYSVLSLFAFPLMYVNGGGSPSSFSDYSDLTGTRTIP
                     FPVDTPVNLPYPFNDRLTDPYSNPYDHSPLYGTTPSNVNTSVEYNPETREYDINEKIG
                     KDFFRNPSYMTFEEFKEDQFRKSTQQYWKERAEGEDAITRKPLIPKIYVAGETFDRIF
                     GGNTIDIRPQGSAELTFGVNINRTDNPQIPEKQRRLTSFDFNEKIQMNVIGNIGEKMK
                     LSVNYNTEASFDFENQMKLEYTGYEDEIIRKIEAGNVTLPLTGTLIKGSQSLFGIKTQ
                     LQFGRLGVTTIFSQQKGQASTINVPPGGGQVTNFDIDGDQYEANRHFYISQYFREHYD
                     EAVSKLPTIVSPISITKIEVWITQVNFNSQDQTRNLVAMQDLGEYNAYASSFVGQGTS
                     VYPSDQLSNNLYQKLTTTYSGIRNFQTASSILDPLSSVYNFSPQQDYELITNAKKLSS
                     TDFTYNEKLGFISLNSELRNDQALAVAFEYTVNGKVYRVGELTTSGIDPSQPLIVKLI
                     RGRNFNTTLPSWKLMMKNVYNLNAFQISSEKFRLDVLYFDNAAGSYVRQLPEPNEPKI
                     ANKSLIRVLNADNLNSTGDATQSGDGIFDYVEGITVNSANGRIILPSVEPFGRYLESK
                     FSNATTAAKYVFKELYDSTKTIALAQGKNKFKLKGSYQSSSGSEISLNAINIPQGSVK
                     VTAGGTELVENQDYTVDYNLGRVKIINTSVLNSATPIKVSLESNSLFSVQSKTLMGAR
                     FDYKFSKDFAVGGTFMHLNERPITRKVNIGDEPISNSIFGLDGTYRTRSRFITKLVDK
                     IPLINTKEESSVSLNGEFAYLLPGHSKAIGKKGNAYIDAFEGTQSTIPLNIAGQWTLA
                     SVPRFQPALFPEFDYVSATNDTLGYGYNRAKMAWYNIDPTAFYRSNSTVSLSAAERSN
                     HNVRQISEKELFPKRQYSNGIVPYMNTFDISFYPDERGPYNYETSARAGLSAGIDANG
                     KLIDPTSRWGGFMRKVETSNFEESNIQYIQFWLMDPFNGDAPDSSMSNTGTLYFNLGD
                     ISEDVLRDGYESYENGLPTTATPAAYRQNAWGRTPVAQNVLYAFDRDESTRKQQDVGL
                     DGLGNDDEKVHFLTNFLDYIDANVTNQTAKDAIHDDPSSDDFRYFRGDAYTNADGILQ
                     RYKNFNGTENNSPVAGSGTFVPSSTQTPDAEDANRINGMEVDENYFQYKVDIDPSKLV
                     VGQNYVSDVLENVAALNLPDASGKTITWYLIKIPVNDPTRERIGQISDLKSVKFIRVF
                     AKGFSGPKVLRLAKFEFLRGEWRKYDYKLTGILSQPAVFDVGAVSLEENSNREPINYV
                     LPPDFEREIDVTSTNQRQLNEQSLSFKVCELADGDMKVCYKNTSLDMRNYKHVKMVIH
                     AESFLPPANPTNDGELYAVVRLGSDFTDNYYDYRIPLKMTPFGSTDPTVIWPKENDFD
                     IDLDKLTDAKLERNNAGVAFTDEYSKLDGNNVIFIKGNPTLSEVKVMMLGLWNPSGEN
                     PAFPPRPPDDGLAKCAEVWFNEFRMSDFNDKGGWATIGRAQVKLADLGNVSVSGGYST
                     HGFGSLESKVGERSKEDITNYDIATSLELGKFFPSSTGLTVPMYFGYGEVFSNPQYDP
                     LDPDIEFSRSISNIDDSKRKQNRKRFSQDYTRRKSLNFTNVKKNKTGKNASKSRIYDI
                     ENFNFTYGYTEVYRRNINTEYDIQKDYLGAIGYNFNTNPKPITPFSKMKGKSKYLRPI
                     KDFNFNYMPSTFNFRWDVNRHYGELKVRDVNAVLNQGVSDNSIPVLYDKRFTMNRQYG
                     TSWDITRALKFEFNANNQSKVDEPDGALNSEEKRDSVRKNFFNLGRNTDYMHNASLSY
                     NLPLNKLPITDWISINTRYSTTYHWTASPLIVNPDNGRYYTNPALGNTIQNSNSIQVN
                     NSLNMTSLYNKWKLYKKLTAPPKPKTPAKKEPKVDAKAPDAAKDSLKTKSVKPPEKKK
                     EQEINPAIKALAKIIFSLKNVSFNWNETNGLLLPGFNKQSEWLGQNWDGNSAPGFAFT
                     FGSQNQDIRERAARNGWLTTDSTFNSQFTSTYTQNITGRASLEPFTGFNIDVNFNRNY
                     SLSTTENYRAFNSPRANGRYYETLARVETGNFSISNLMWGTSFTKDRKDYSSTIFERF
                     AENRRILSERLGNENPNSSGVGADGYRDGYSGTSQEVVILSFLSAYSKKSASRMSLDL
                     FPSVPLPNWTVRYDGLTKIAFVKNLFQSVNLNHGYRSTYNVNSFTTDVSYVPGGNARD
                     INMDFIPKRQIGQVSLSEQFSPLIGIDITWKNRNDKSSGSKSATERGGRSSGGNFTTR
                     FEYKRDRNISLTLAGIQVTEVKGREFTFGAGYRIPNFKFPFGLFKSVNSRRSNDLNTT
                     LDFSVRKNTTILRKLVEGTNQPTAGLTIISIKLASDYTVSERLNLRLFFEKTINTPVV
                     STSYPNANTAFGITIRFTLSQ"
     gene            complement(7611..8195)
                     /gene="ruvA"
                     /locus_tag="PKY09_08940"
     CDS             complement(7611..8195)
                     /gene="ruvA"
                     /locus_tag="PKY09_08940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNB13023.1"
                     /translation="MYDYISGKITHATATYLVVEANGVGYQLQVSLQTYSKLKDAQSA
                     KVYTHLSIKEDAHLLFGFADEDERQLFRHLISVSGVGPGTARVMLSSLSTTELQRAIT
                     TGNVAVIKKIKGIGDKTAQRIVIDLKDKLGKAGTTAPVLLTQGNTIKEEALSALLTLG
                     FNRNVAEKELNKVMAGGGELQSVEVLLREALKNL"
     gene            complement(8879..11140)
                     /locus_tag="PKY09_08945"
     CDS             complement(8879..11140)
                     /locus_tag="PKY09_08945"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNB13024.1"
                     /translation="MSVNKEALDYHASGRPGKIEVIPSKPYSSQRDLSLAYSPGVAEP
                     CLEIEKDIDNVYKYTAKGNLVAVISNGTAVLGLGNIGPEASKPVMEGKGLLFKIFADI
                     DVFDIEINQTNVDDFIKTVKAISPTFGGINLEDIKAPECFEIESRLKNELNIPVMHDD
                     QHGTAIISGAALINALDIVNKKINHVKIVVNGAGASAISCTKLFVSLGAQKKNITMLD
                     STGVIRKDREGLDVNKSEFATDRNINTLEEAMHGADVFIGLSKGNILSADMLKSMADK
                     PVVFAMANPNPEITYPDAKSAREDVIMATGRSDFPNQVNNVIGFPFIFRGALDVRATK
                     INEEMKLAAVHAIAQLARQPVPEMVNLAYNAKNLQYGPEYIIPKPLDPRLIYTVAPAV
                     ARAAMETGVAQVSITNWEHYVEDLKKRLNLDNKLIRAIVTRAKQNPQRIVFAEADNYK
                     ILKAAQQVKEEGIARPILLGNRERIKQIVTENHIDITDIPIVDPRIDTEHLNTFGELF
                     FEKAKRRGFSVYEAKKVMRERNYYGAMMVETGQADALISGLTRNYADTIRPALRIIGV
                     QEHGTRVAGMYIMMTKKGPLFFADTTINVNPTSEDLVEITLLTAKAVQQFNIKPRIAL
                     LSFSNFGTSNDPSAVRIRKAVEILHDKFPGMIVDGEMQANFALNMQMLHDSYPFCELI
                     NGGVNTLIFPNLDAGNIAYKLLQSMEAAEAIGPVLLGMKKPVHILQLGSSVRDIVNMV
                     TIAVIDAQSRKQQ"
     gene            complement(11247..11750)
                     /gene="ribH"
                     /locus_tag="PKY09_08950"
     CDS             complement(11247..11750)
                     /gene="ribH"
                     /locus_tag="PKY09_08950"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNB13025.1"
                     /translation="MALYQENNSLYNPAEYPDAFSVKVGIVTASWNNKITSKLFDGAV
                     EVLKKSSVKENNLISIQVPGSYELTAGAQWLFQHHSPDVVIALGCVIQGETRHFDFIC
                     HAVANGLNHVSLKFGKPVIFGVLTTDNEQQALERCGGTHGHKGREAAFTALSMALLKT
                     PAAKIGF"
     gene            complement(11757..12431)
                     /locus_tag="PKY09_08955"
     CDS             complement(11757..12431)
                     /locus_tag="PKY09_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13026.1"
                     /translation="MTDKKSTGFDVEESFDRAESYIHENKKSLTIILVVAVLLVGGYF
                     AYNQFIVKPQQANAEKQMFMAEYYFKNDSLDKAIKGDGNFPGFEEIISSYGSSKSANL
                     AHYYLGVSLLRKGQYDDAIAALSKYDAEDDITGAIALGCIGDAYMEKGNKEEAMSFYK
                     KASDYDQNQFTAPIYLMKQAMIFELNNDYKSAYDIYNRIKRDFPNSTEARQIATYMAR
                     AEAHMN"
     gene            complement(12521..13396)
                     /locus_tag="PKY09_08960"
     CDS             complement(12521..13396)
                     /locus_tag="PKY09_08960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNB13027.1"
                     /translation="MAISIKTRLLDTIRAVNKIPMVEKWLVQQSQNKHSIARKLVGSQ
                     YLYKPGTIRNCTRNGINYQLHINDYLDHGIYFGMQDHIDFDRESLLGFIKPDSYILDI
                     GANIGDTTLQMAKKLNGRGQIFSFEPSPAVFERMKTNVSLNSFKNITLFNEGMGDEVG
                     VLNLISHGNNHSGGAFISKDANNAIKVAVTTIDKFVAEHNLSKLDFLKIDTEGFEVFV
                     LKGGVNTFRNLKPEMFIEVSESLLKRAGSSAKELIHILNELNYQCIRVDTQENITEDY
                     NFAEQHFDIYCRPIK"
     gene            complement(13558..>14409)
                     /locus_tag="PKY09_08965"
     CDS             complement(13558..>14409)
                     /locus_tag="PKY09_08965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB13028.1"
                     /translation="AVRYTGTFGIDALLYCQGMKKLLLENGVEIYESSEVVKIEDHCV
                     KTHLGSVKAEQIIFCADKLQHELTHYADKVYHAQTFLSVSEPMSDAMITDMFPAGKFQ
                     CWDTDVVYSYFRLLGSNRLLLGGGDMLTTFSKQDVNSSIVIDRVIRKFKQKFPKLKDL
                     EFVQYWPGRIDCTRDLLPTIARDENKKWLHFVLGCVGLPWATFCGDFAARHAYDDAAC
                     NDHHYYNYLRPDRGFFVPLWLEKVLSKQIVFSLNNGWAKYYQVDINAAETKQITRMRE
                     AENANLQ"
     assembly_gap    14410..14509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14510..14790)
                     /locus_tag="PKY09_08970"
     CDS             complement(<14510..14790)
                     /locus_tag="PKY09_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNB13029.1"
                     /translation="MKLQNWWFTTLLGTEESILPPLKGDIKADVVIVGAGAAGLAAAH
                     RLMDKQLKVVILEKNICGGDTSGKSAGFLTPDSELELSQLIRRFGITGAS"
     gene            14997..15467
                     /locus_tag="PKY09_08975"
     CDS             14997..15467
                     /locus_tag="PKY09_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB13030.1"
                     /translation="MVLNFKIERTTSQDFNFLKLVAELDKDLKIKNGTKNDFFAQYNK
                     SDTINHVIVAYIKEKPVGCGAFKEFDSDTVELKRMYVVPDFRCQGIASEILNELEKWA
                     SELQYKKCILETGDKMKEAIGLYQKHQFVKTKNYGQYENITSSLCFKKSIGNKN"
     gene            15521..16111
                     /locus_tag="PKY09_08980"
     CDS             15521..16111
                     /locus_tag="PKY09_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNB13031.1"
                     /translation="MNKTRLEAFSDGVLAIIITIMVLELKVPHETSIKALLILWPVFF
                     SYLISFIYVGIYWGNHHHLLHTVHHVNGKIIWANMGLLFCLSLLPFTSAWMGENIPEK
                     IPLMLYSINLAVCAVAFYFLQMCVLPQYKTTSKLIDAVKKQKTKGMISLGIYIAAAVV
                     SFIMPWLSIILIIIPAILWVIPDKNIEEAFVEEHKE"
     gene            16363..17325
                     /gene="ric"
                     /locus_tag="PKY09_08985"
     CDS             16363..17325
                     /gene="ric"
                     /locus_tag="PKY09_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNB13032.1"
                     /translation="MQTEVDNVLNVTLLEPRMKHPTIFARFDELNPGESLTIHNDHDP
                     KPLYYQLLGERGNIFTWEYLEQGPQWWKVKISKRISGESDETLGEIVAKDMHKAQIFK
                     KYGIDFCCGGKKTVKEACEEKGIDVTLVEQELQKAGKVVSNRPLPYNDWNLDFLADYI
                     VNTHHTYVKNNLPEISGYAAKVARVHGSNHPELLRINQLVEAISSEFSSHLVKEEQVL
                     FPYIKRMVEAKNNKESVQQSSFGSVKGPINMMEIEHESAGKNMSEIRTLSNDFTLPED
                     ACASYSLLFRLLEEFEEDLHTHVHLENNILFPKALALEKELVTK"
     gene            17590..17913
                     /locus_tag="PKY09_08990"
     CDS             17590..17913
                     /locus_tag="PKY09_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNB13033.1"
                     /translation="MENTIKPALQAWTSGRVSGFYGKELLNLNNGTVKLVKVDPHAKY
                     PEHIHPDKTEYAFVLEGNPELFIEGSHYSGQPGEFFIFPSAKKHAIQNNTPSECTLLI
                     GAITV"
     gene            17972..18778
                     /locus_tag="PKY09_08995"
     CDS             17972..18778
                     /locus_tag="PKY09_08995"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNB13034.1"
                     /translation="MTINANTKIATILKQHPDALDVIVSISPKFEKLRNPLLRKIMAG
                     RTSLAMASKIGGCSVNDFFVKLQPLGFEIDSTTQEIEKAAAGKMPEIFTSLKKEQIVS
                     LDVRPVLAAGSDPLNLILENVNSLEAGKCLKIINTFEPTPLIIMLEKKGFVTYTDTIN
                     DELIETYFYKKSDSKKEINPVINTSGGWDEIIQQYNNKLQTIDVRALEMPLPMLTILE
                     ALEKLPSDHALYVYHKRIPVFLLPELAEKKFDYRIKEISDGEVHLLIFKS"
     gene            18790..>19687
                     /locus_tag="PKY09_09000"
     CDS             18790..>19687
                     /locus_tag="PKY09_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HNB13035.1"
                     /translation="MIITDSIKTTSYKVVLPFYIYAAFAFLLSAILLLTSSSAFTDHY
                     FQPHILAITHLMALGWGTMIILGASHQLVPVLIEGKLYSNALAYTSFILAGIGIPLLV
                     YGFYVFNMHNPAKWGGRFILLSIIAYLINLGVSMSRSKKENIHALFVFTSVLWLFLTG
                     ALGLALVYNFTYNMFPHDSLHYLPLHVHAGVIGWFLMLIIGVGSRLIPMFLISKYTNT
                     KLLKWIFVLINGALISYILIFYFIKITEVILLPWLMLFTGIALFMYYCYSAFKHRIRK
                     QVDEPMKVSLLAVMLIMIPLVLL"
BASE COUNT         5875 a         3714 c         3665 g         6333 t
ORIGIN      
        1 cttcgtacac acttgaaaag actcaaccgc agaacaattt gttttacacg cagtgtaata
       61 atgctatctg catgtttgaa aatttatttt tgggggtgaa taatctattc ccccccccct
      121 attgcgacag tgtaaacctt atcgttatac caaatgctgt gttggcattg gggtacgatg
      181 tagaaactac tggtgtgttg attgttttct cgaagaacaa tcttaaatta agtcgttcac
      241 tcactgtata atcagaagcc agtttaatag agatgatggt taaacctgct gtaggctgat
      301 tggtgccttc aaccaatttt ctgaggatag tagtgttctt gcgaactgag aaatctaatg
      361 ttgtattcag atcgtttgat ctgcgtgagt taacactctt aaacaatcca aatgggaact
      421 taaagtttgg aatacgatag cctgcaccaa aagtaaactc ccgtccttta acttctgtta
      481 cctgaatgcc tgccagtgtg agagaaatat tacgatcgcg tttgtactca aatcgtgtgg
      541 tgaaatttcc accggaagaa cgtccgccac gctctgttgc agatttgctt cctgatgact
      601 tatcgtttct gtttttccag gtaatgtcta ttccaattaa tggactaaat tgttcgctca
      661 aactcacctg ccctatctgt cgctttggaa taaaatccat attgatatcg cgagcattac
      721 caccgggaac ataagaaaca tcggtagtaa aactgttcac attataagtt gaacggtaac
      781 cgtggttgag gtttacactc tggaaaagat tttttacaaa tgcaattttg gtaagtccgt
      841 catatcgcac tgtccagtta ggaagtggta ctgatggaaa caaatcgagc gacatgcgtg
      901 atgcactctt tttagaatat gctgaaagga aagaaagaat cactacctct tgtgatgttc
      961 cactgtaacc atctctgtag ccatctgcac caacacctga agagtttgga ttttcatttc
     1021 ccaatctttc agaaagtatt cttctgtttt ctgcaaatcg ttcaaaaatt gtagatgaat
     1081 aatcttttct gtctttcgtg aatgatgttc cccacatcag attgctgata ctgaaattgc
     1141 cggtttctac tcttgctaat gtttcataat aacgaccatt agcacgagga ctgttgaagg
     1201 ctctgtaatt ttctgttgtg cttaaagagt aattacggtt aaagtttaca tcaatattaa
     1261 atcctgtaaa aggttcaagc gatgctcttc ctgtaatgtt ttgtgtatag gtagaagtaa
     1321 actgactgtt aaaagttgag tcagttgtaa gccagccatt cctggccgct cgttctctga
     1381 tgtcctgatt ctgactacca aaagtaaatg caaacccggg tgcactgttg ccatcccaat
     1441 tctgacccaa ccattcactc tgtttgttga agccgggcaa caataatcca ttggtttcat
     1501 tccagttaaa cgaaacattt tttaaactga aaataatttt tgccaatgct tttattgccg
     1561 gatttatttc ctgttccttt ttcttctccg gtggcttaac actctttgtc tttaaactgt
     1621 cttttgcagc atccggagct ttagcatcaa cctttggctc tttcttagca ggtgtttttg
     1681 gctttggagg tgcagtcaac ttcttataca gtttccactt attatataaa gaagtcatgt
     1741 ttaagctatt gttaacctga atagaatttg aattttgaat cgtgttaccc aaggcagggt
     1801 ttgtataata ccttccatta tcagggttca ctattaaggg tgatgctgtc cagtgataag
     1861 tagtgctgta gcgtgtgttt atacttatcc agtctgtaat cggcaactta tttaatggaa
     1921 gattatacga tagagatgca ttgtgcatgt aatctgtatt tcgacctaag ttgaaaaagt
     1981 tcttccgcac agaatcgcgt ttctcttcag agtttaaagc accatcgggt tcatcaactt
     2041 tactctgatt gttagcatta aattcaaact tcaatgcgcg tgtaatatcc cagcttgtac
     2101 catactgacg attcatagta aaacgtttat catacaatac aggaatagag ttatcagaaa
     2161 caccctgatt taatactgca tttacatcgc gtactttaag ttcgccataa tgcctgttta
     2221 catcccatcg gaaattaaag gttgacggca tgtaattaaa attaaaatct tttataggac
     2281 gcaggtactt cgacttacct ttcattttac tgaaaggggt aatcggcttg gggttggtat
     2341 taaaattata ccctattgca ccaagatagt ctttctgaat gtcatattct gtattgatat
     2401 ttctccgata gacttcagtg tagccataag taaaattaaa gttctctata tcataaattc
     2461 tggacttgga tgcattctta cctgtcttgt ttttcttgac attggtaaag ttcaaactct
     2521 ttctccttgt ataatcctga gaaaaacgtt ttctgttttg cttgcgcttt gaatcatcaa
     2581 tgttgctgat tgaacggctg aattcaatat ccggatcaag aggatcgtac tgcgggttgc
     2641 tgaatacttc accataacca aaatacattg gcactgtcag acctgtactt gaagggaaaa
     2701 acttacctaa ctcaagtgaa gtggctatat catagtttgt aatatcttcc ttgctgcgtt
     2761 caccaacctt actttctaaa cttccgaaac catgagttga atagcctccc gaaacagaaa
     2821 cattacccaa atctgcaagt tttacctgtg cacgaccaat ggtggcccag ccgcctttat
     2881 cattaaagtc agacatacgg aattcattga accatacctc tgcacacttc gccaatccgt
     2941 catccggtgg tcgtggtggg aatgcaggat tctcgccact tggattccac aatcccagca
     3001 tcataacttt tacttcgctc aatgtggggt ttccttttat aaaaattaca ttgttgccat
     3061 ccagcttcga gtactcatct gtaaaagcaa caccggcatt attacgttcc agcttggcat
     3121 cggtcagttt atccaaatca atatcaaaat cattttcttt aggccagata actgttggat
     3181 ctgtgcttcc aaatggtgtc attttcaatg gaatacggta gtcgtaatag ttatctgtaa
     3241 agtcagagcc taaacggaca actgcgtaaa gctcaccatc gttggttgga tttgccggtg
     3301 gcaaaaacga ctcggcatgg ataaccattt ttacgtgctt gtaattacgc atgtcgagtg
     3361 atgtgttttt ataacaaacc ttcatgtcac catcagcaag ttcgcaaact ttaaatgaaa
     3421 gcgattgctc gttaagctga cgctgattgg ttgaggtaac atctatttca cgttcaaaat
     3481 caggtggcag aacatagtta ataggctcgc ggttactgtt ctcttccagc gaaacagcac
     3541 ctacatcaaa cacagcaggc tgactgagaa tacctgtaag tttataatca tactttctcc
     3601 actcacctct caggaattca aattttgcaa gacgcagcac cttgggtcca ctaaatcctt
     3661 ttgcaaaaac acggataaac tttaccgact ttaaatcgga aatctgtccg atacgttcgc
     3721 gtgttggatc gttaaccgga attttaatca gataccatgt gatggtcttg cctgatgcat
     3781 caggcagatt taatgcagct acattttcca acacatcgct gacatagttt tgaccaacaa
     3841 ccaattttga aggatcaatg tcaactttat attgaaaata attttcgtca acttccatac
     3901 cgttgatgcg gttggcatct tctgcatccg gagtttgcgt gcttgacggc acaaaagttc
     3961 cgcttcctgc taccggtgaa ttattttctg ttccgttaaa gtttttataa cgttgtaaaa
     4021 ttccatcagc attggtataa gcatcacctc tgaaatatct gaaatcatct gacgaagggt
     4081 cgtcatgaat ggcatctttt gcagtttgat ttgttacgtt ggcatctatg taatcaagga
     4141 agttggtgag gaaatgtact ttctcgtcat cattacctaa accatctaaa ccaacatctt
     4201 gctgtttacg tgtactttca tctctgtcga atgcataaag aacgttttgt gcaacaggcg
     4261 tgcggcccca agcattctga cggtacgctg ccggtgttgc cgtagttgga agaccattct
     4321 catagctctc atatccatcg cgcaacacat cttcagagat atcacccaaa ttaaaataaa
     4381 gtgttcctgt gtttgacata ctggaatcag gcgcatcacc attaaatggg tccatcagcc
     4441 aaaactgaat gtactgaatg tttgactctt caaagttgct ggtttctacc tttcgcataa
     4501 aaccacccca acgtgatgtt ggatcaatca atttaccatt tgcatcaata ccggcagata
     4561 agcctgcacg ggcagatgtt tcataattgt aaggtccacg ctcatcagga taaaaagaaa
     4621 tgtcgaaagt gttcatgtaa ggcacaatac cattgctgta ttgcctcttg gggaacaatt
     4681 ccttttcact gatttgtctt acgttatgat tacttctttc tgctgccgat aatgacaccg
     4741 tactgttact tctgtaaaaa gctgtcgggt caatattgta ccatgccatt tttgcacgat
     4801 tgtaaccata tcctaacgta tcgtttgtgg cactcacata atcaaattcc ggaaacaatg
     4861 caggctgaaa acgtggcacc gatgctaacg tccactgtcc tgcaatattc aaagggatgg
     4921 tactctgtgt tccttcaaaa gcatcaatat aggcattccc ttttttaccg attgccttac
     4981 tatgtccggg aagcaaataa gcaaattcac cgttcaatga aactgatgat tcttccttcg
     5041 tattaatcaa tggaatctta tcaaccaact tggtgatgaa tcgtgagcgt gtgcgataag
     5101 ttccatccaa accgaaaatt gaatttgaga taggctcatc accaatgtta acttttcggg
     5161 tgataggtct ttcgttgagg tgcataaagg taccgccaac agcaaaatct ttactgaact
     5221 tgtagtcaaa ccgtgcgccc atcaatgttt ttgactgaac agaaaacaag gaattactct
     5281 ctaaagaaac ttttatgggt gttgcagaat taagcaccga agtgttgatg atttttaccc
     5341 gacctaaatt ataatcaaca gtataatcct gattttctac taactctgtt ccaccagcag
     5401 ttactttaac agagccttgc ggtatgttga tggcattaag tgaaatttca gaacctgaag
     5461 aactctgata tgagccttta agcttaaact tgtttttacc ctgtgctaaa gcaattgttt
     5521 tggttgaatc ataaagctct ttaaacacat acttcgctgc cgttgtagca tttgaaaatt
     5581 tcgattcaag ataacgcccg aatggttcaa cagagggcag aataattctt ccgttagcac
     5641 tatttactgt tataccttct acataatcaa aaataccgtc accggattgt gtagcatcac
     5701 cggtagaatt tagattatcg gcattgagta ctctgataag acttttattg gcaattttgg
     5761 gctcgttagg ctcaggcaac tgacgcacat aacttccggc agcattgtcg aaataaagaa
     5821 catctaatct gaatttttcg cttgaaatct gaaaagcatt caggttataa acgtttttca
     5881 tcatcagttt ccatgatgga agtgttgtgt taaaatttct tcctctgata agttttacaa
     5941 tcaatggttg cgatgggtca atacctgaag tcgtaagctc acccactctg taaactttgc
     6001 cgttaacggt atattcaaat gcaacagcca atgcctggtc gttacgcaac tctgagttga
     6061 gcgaaataaa acctaacttt tcattgtatg taaaatctgt gctgctgagt ttctttgcat
     6121 tggtaattaa ctcataatcc tgctgtggcg aaaaattata aactgaagac aatggatcga
     6181 gtatagagga tgctgtttga aaatttcgaa taccgctgta tgttgttgtg agtttctgat
     6241 aaaggttgtt tgataactga tctgatggat aaacagatgt gccttgccca acaaaagagg
     6301 aagcataagc attatactca cctaaatcct gcatggctac gaggtttctt gtctgatctt
     6361 gtgagttgaa gtttacctga gtaatccaga cttctatttt ggtgatgctg atgggtgaaa
     6421 caatagtagg cagcttgctt actgcttcat cataatgctc tctgaaatat tgcgaaatat
     6481 aaaagtgtct gttggcttca tactgatcac catcaatatc aaaattagtg acctggccac
     6541 ctcccggagg tacattgata gtgcttgcct gccctttttg ctgtgagaaa attgttgtaa
     6601 caccaagtct tccaaactgc aactgtgttt ttatcccgaa caaactctgg ctacctttga
     6661 ttaaagttcc cgttagtggt aaggtaacat tacctgcttc tatttttctg ataatctcat
     6721 cttcatagcc tgtatattca agcttcattt gattttcgaa atcgaaactc gcttctgtgt
     6781 tgtagttgac actgagtttc atcttctctc ctatgtttcc aatcacattc atttgaattt
     6841 tctcgttaaa atcaaaagat gtaaggcgtc tttgtttttc gggaatctgc ggattatcgg
     6901 tgcggttgat gttaactcca aaagtcaatt ctgcagagcc ctgcgggcga atgtctatgg
     6961 tgttgccacc aaatatcctg tcaaatgttt cacctgcaac ataaatttta ggaattagcg
     7021 gttttcgggt tatggcatct tcaccttctg ctctttcttt ccaatattgc tgtgttgatt
     7081 tacgaaactg atcttcttta aactcttcga acgtcatgta ggaagggttg cggaaaaaat
     7141 ctttgcctat tttttcatta atgtcgtact ctcttgtttc ggggttgtat tcaactgagg
     7201 tattaacatt ggaaggtgtg gttccgtaaa gcgggctatg atcgtaaggg ttggaatatg
     7261 ggtcggttaa cctgtcgtta aaaggataag gaaggttaac aggagtatca acaggaaatg
     7321 gaatggtgcg tgttcctgta aggtctgaat agtctgaaaa agaagacggg ctgcctccgc
     7381 cattgacata catcaacgga aatgcaaaca aacttaatac tgaataacga agcaatttta
     7441 gtccttgcaa aatatctgat atttggggtt cctacaaatg agtaataaaa aaatttccgg
     7501 ctaaaaataa aacaaaaaat caaacggttt aaaggtaaaa atcaacagtt acggacactt
     7561 ttttaatgct atattttggt taaaatgaag tatttttaag aggttcgtat ttataaattt
     7621 tttaaagctt ctctcagcaa aacctcaaca gactgaagct cgccaccacc ggccatcact
     7681 ttatttagct ctttttcagc cacatttctg ttaaagccga gagtaagcaa agcacttaac
     7741 gcctcttctt taatagtatt gccttgtgtt aataaaaccg gtgcagtggt gcctgccttt
     7801 cctaacttgt ctttaaggtc tatgactatg cgctgtgcgg ttttgtcgcc tatgccttta
     7861 atttttttaa ttacagccac attgcctgta gtaatggctc tttgtaattc tgtagtgctg
     7921 agcgatgaaa gcatgactct ggcagtgccc ggacctacac ctgatactga aataagatga
     7981 cggaaaagct gtctttcatc ttcatcggca aaaccaaaaa gcaggtgtgc atcttctttg
     8041 atagatagat gcgtataaac tttagcactt tgtgcatctt taagttttga ataggtttgc
     8101 agtgagacct gaagctgata ccctacacca ttggcttcta caacaaggta ggtggctgtt
     8161 gcatgggtta tttttcctga aatataatcg tacatttttt tataaatttt tctgctgctt
     8221 taaaatcgcg acctcaaacg tacggaaagt tggcgagttg ataaattact gaactaatga
     8281 attgcagcgg gcactttcat gcctgtttcc ttttgccgtt tgcgtttttt cacgccatgc
     8341 tggttttctt ccctgatatt ccaacaccca atcgcaaatg ttgcgaaatg gaataccata
     8401 cttttatcca agttccccct tagctgtagt tgcctgttaa atactcggca acaatttgct
     8461 cttttgttct tcttttcttg acacattttg tgcgaaataa agttaataat tctgtcaacc
     8521 tatttcagaa aaagtcattt cgcttttttc gccattcttc ggtcttaata tgtaaagttt
     8581 ttatagtctg ctaagttaag ttttatatcg tcattgtcgt tattgatgtg tatgcagaaa
     8641 gcaaagtact taacgtacaa tgtaaaaata aaaatgatat ggtgagaata taaaaggtca
     8701 aagaaaatac agcatgaaaa tgttctatgc ccatgctatt agccctgatg tatttctgca
     8761 aagtttttgt gtatgagcat tgccatgcag cggggcggca gcaagaggta atgcatgatg
     8821 aggttacagt agttagtact tcaaaaaaaa ttaattgctc aatgacaaag ataaaccact
     8881 actgttgttt tctgctctgt gcgtcaatga cggcaatggt taccatgttt acgatgtcgc
     8941 ggacactgct gccgagttgc agaatgtgta caggtttttt cataccaaga agtacgggac
     9001 cgatagcctc tgctgcctcc attgactgca gtagtttgta tgcaatgttt ccggcatcga
     9061 ggtttggaaa gatgagtgtg ttgacaccac cgttgatgag ttcgcagaaa ggataactgt
     9121 catgcagcat ctgcatgttg agtgcaaagt ttgcctgcat ttcgccatct actatcatgc
     9181 cgggaaattt gtcgtgaagt atttctacag cttttctgat acgtacggca gaggggtcat
     9241 ttgatgtacc gaagtttgag aatgacagga gtgcaattcg cggcttgatg ttgaattgct
     9301 gaacagcttt ggcagtgagc agggttattt caactaaatc ttctgatgtg gggtttacat
     9361 taatggtggt gtcggcaaag aacaaggggc ctttttttgt catcatgata tacatacctg
     9421 caacgcgtgt tccatgttcc tgcacaccaa tgattcgtag tgcagggcga atggtgtcgg
     9481 cataattacg tgtgagaccg gaaataagtg catcggcctg tccggtttct accatcatgg
     9541 caccatagta gtttctttcg cgcattactt ttttagcttc gtaaacggag aaacctctgc
     9601 gttttgcttt ttcgaaaaac agttcgccaa aggtattgag atgctctgtg tcaatacgcg
     9661 ggtcaacaat aggtatgtcg gttatgtcaa tgtgattttc tgtgacgatt tgttttatgc
     9721 gttcgcggtt gcccaacagg ataggccgtg caatgccttc ttctttgact tgttgtgcag
     9781 ctttcagaat tttgtagttg tcggcttctg caaagactat gcgctgcgga ttttgcttgg
     9841 ctcttgttac gatggcacga atgagtttat tgtcgaggtt gaggcgcttt ttgaggtctt
     9901 ctacatagtg ttcccagttg gtaatgctaa cctgtgccac acccgtttcc atagccgcac
     9961 gtgcaacggc aggtgcaacg gtataaatca atcttgggtc gagtggtttg gggatgatat
    10021 attcgggacc gtattgcaga ttttttgcgt tgtaggcaag gtttaccatt tctggaacgg
    10081 gctgtcgtgc caactgcgca atggcatgca ccgctgccag tttcatctct tcgttgattt
    10141 ttgttgcacg aacatctaat gctcctctga atataaatgg gaaaccgatt acgttgttta
    10201 cctgattggg aaaatcgctg cgacctgttg ccataattac atcttcgcgt gcactcttgg
    10261 catcgggata agtaatttcc ggatttggat tggccatagc aaaaactaca ggtttgtcag
    10321 ccattgattt gagcatgtct gcactcagga tatttccttt gctgagaccg ataaatacat
    10381 cggcaccatg catagcttct tcaagtgtgt taatgtttct gtcggttgca aattcgcttt
    10441 tgtttacgtc taatccttca cggtcttttc tgatgacacc ggtactgtcg agcatggtga
    10501 tgtttttttt ctgtgcacca agcgaaacaa aaagtttggt acatgagatt gctgaggcac
    10561 ctgcaccatt gacaacaatt tttacatgat tgattttttt gtttacaata tccaacgcat
    10621 taatgagtgc tgcacctgag atgatggcag tgccatgctg atcgtcatgc ataacaggaa
    10681 tattcaactc attttttaag cgactttcaa tttcaaaaca ttcaggagct ttaatgtctt
    10741 cgaggtttat accaccgaat gttggtgaaa tggcttttac tgtttttata aaatcgtcaa
    10801 cgttagtttg attgatttct atatcgaaca catcaatgtc tgcaaatatt ttaaacaaaa
    10861 gccccttacc ttccatgaca ggctttgatg cttcggggcc aatgttgcca aggccaagaa
    10921 cggcagtgcc gttagaaatt acggcaacaa gatttccttt ggctgtgtat ttataaacgt
    10981 tgtcaatgtc tttttcaatt tcaaggcaag gctctgcaac accaggcgaa taggctaagg
    11041 ataaatcgcg ttgtgagctg tatggtttac tgggtatcac ttcaatttta ccgggacgac
    11101 cggatgcatg ataatcgagt gcttctttat taactgacat agttattttt tttcaatgtg
    11161 ctaaagtaat gaatacagta tcacgttatg gcagtaaata aacagatgag atactgaatt
    11221 aaatcagctt aaattctata ttaaatctaa aaaccgattt tggcagcagg tgttttgagg
    11281 agtgccatac tcaatgcagt gaatgctgct tcgcgtcctt tatgtccatg tgtgcctcca
    11341 caacgctcaa gtgcttgttg ttcgttgtcc gtagttaaaa caccaaaaat tacaggcttg
    11401 ccaaatttta atgatacatg attcagacca ttggcaactg catgacagat aaaatcaaaa
    11461 tgacgtgtct cgccctgaat gacacaaccc aaggcaataa caacatccgg tgaatgatgt
    11521 tgaaaaagcc attgcgcacc ggcagtgagt tcgtagctgc ccggaacctg aatggagatg
    11581 agattgtttt cttttacaga tgactttttt aaaacctcaa ctgcaccatc aaaaagtttt
    11641 gaagtgattt tgttgttcca gcttgctgta acaattccta cttttactga aaaggcatca
    11701 ggatattcag cggggttata aagagaattg ttttcctgat ataatgccat gaaatattag
    11761 ttcatgtgtg cttctgcacg cgccatgtag gttgcaatct gacgtgcttc ggtagaattc
    11821 gggaagtctc tttttattct gttataaata tcataggcag atttatagtc gttgttcaac
    11881 tcaaaaatca ttgcctgttt catcagataa ataggtgctg tgaattgatt ctgatcataa
    11941 tcagacgctt ttttataaaa gctcatagct tcttctttat tgcctttttc catgtaggca
    12001 tcaccaatac atcctaatgc aatggctcct gttatatcat cttccgcatc gtatttagat
    12061 aaagcagcaa tagcatcatc atactgtcct ttgcgcaaca aacttacacc aaggtagtag
    12121 tgtgccagat tagcagactt tgatgaacca tagcttgaaa taatttcttc aaagccggga
    12181 aaatttccat cacctttgat cgctttatcc aatgaatcgt ttttgaaata atattctgcc
    12241 atgaacattt gtttctcggc attagcctgc tgtggcttta cgataaattg attgtatgca
    12301 aaatatccgc caaccaataa tactgcaaca acaagaatta tagttaaact ttttttgttt
    12361 tcgtgtatat aggattctgc cctatcaaag gattcttcta catcaaaacc ggttgatttt
    12421 ttatcagtca ttgtttctgc aaaaatttga gcgggcaaag atagtttttt ttctgaaata
    12481 acaggcatac aaatataacg cctaatttac tcaatttcaa ttatttaatt ggcctgcagt
    12541 aaatatcaaa atgttgttcg gcaaagttat aatcttcagt aatgttttcc tgagtgtcaa
    12601 ctcttataca ttggtaattt aattcattta atatatgaat gagctcttta gcagaggagc
    12661 ctgctctttt aagcaatgac tcgctgactt caataaacat ttcaggcttt aaatttcgaa
    12721 aggtatttac accacctttc aggacaaaaa cttcaaatcc ttctgtatcg attttcagaa
    12781 aatcaagttt tgacagatta tgttcagcaa caaacttatc aatcgttgtg acagcaactt
    12841 ttatagcatt gtttgcatct ttactgataa aggctccacc ggaatgattg tttccatgag
    12901 agattaaatt taatacgcca acctcatcac ccattccttc attaaaaaga gtaatgttct
    12961 taaagctatt gagacttaca tttgttttca ttctttcaaa aacagcaggt gatggttcaa
    13021 atgaaaatat ttggccacgc ccatttaact ttttggccat ttgcaatgtg gtatctccta
    13081 tgttggcacc tatgtccaga atgtacgagt ccggtttaat aaaaccgaga agactttctc
    13141 tgtcaaaatc aatgtgatct tgcattccaa aatagatacc atgatcaaga taatcgttaa
    13201 tgtgtaattg atagttaatt ccatttctgg tacaattacg tatggtgccg ggtttgtata
    13261 aatactgact accaactaat tttcgtgcaa tgctatgttt gttttgtgat tgttgaacta
    13321 accatttttc caccatcggt attttattta ccgcacgtat tgtatctaac aatcgagtct
    13381 taattgatat tgccattcta taatgtcact ttaaaaaaca aaaatataga aatggcagca
    13441 gataggtatc aatttaaata ctactaattc tctgaaagta tattactcaa ttgaataacc
    13501 ctattaagat ttttttatgt tgaaggtagt ttgctaaaaa tcgcaaataa taacaggcta
    13561 ttgcagattt gcattttcag cttcacgcat acgagtgatt tgttttgttt cggcagcgtt
    13621 aatatctacc tgataatact tagcccaacc attgttgagt gaaaaaacta tttgcttact
    13681 cagaactttt tcaagccaaa gtggaacaaa aaaacctctg tcggggcgta gatagttata
    13741 ataatgatga tcgttacaag cagcatcatc ataagcatgt cgtgcagcaa agtcaccaca
    13801 aaaagttgcc cagggaagtc ctacacaacc taatacaaaa tgcaaccatt ttttattttc
    13861 gtcacgtgca atagttggca ataaatcacg cgtacaatct atgcgtccag gccagtactg
    13921 aacaaattcg agatctttta atttcggaaa tttttgtttg aattttctga taacacggtc
    13981 aataactatt gaggagttaa catcttgttt tgaaaaggtt gtcagcatat ctcctccacc
    14041 taacaataaa cggttgcttc ccaaaagtct gaagtaggaa taaacaacat cagtatccca
    14101 acactgaaac ttgcctgctg gaaacatgtc ggtaatcatg gcatcactca tgggctcact
    14161 aacagaaagg aatgtttgtg catgatatac cttgtctgca tagtgtgtca attcatgttg
    14221 taatttatcc gcacaaaaaa taatctgttc agctttaaca gagcctaagt gtgttttaac
    14281 acaatgatct tctattttaa ccacttctga cgactcataa atttcaacac cattttcaag
    14341 caacagcttt ttcatcccct gacaatacaa aagtgcatca ataccaaaag tgcctgtata
    14401 acgaacggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aagcacctgt
    14521 aattccgaat ctgcgaatca gctgtgacaa ctccagttca ctgtcgggag tcaagaatcc
    14581 ggcactttta cctgatgtat cgccaccaca aatattcttt tcgagaataa caactttcag
    14641 ctgtttatcc atgagcctgt gtgcagcagc caggcctgca gcacctgcac cgacaataac
    14701 aacatcagcc ttaatgtcac ctttaagtgg tggcagaatt gattcttctg ttccaagtaa
    14761 ggtagtgaac caccagtttt gtaatttcat atagatacaa tttaaaaaat gggtaatctg
    14821 tagtttttga ggttaattgt attcagcaaa cttaaatttg ttttctgaaa aatatattaa
    14881 tgtaaaataa attaacaaat agcagagcaa aaggtagcta tatataaagc ttcaaccttt
    14941 ttattcaatt ttaaagttgt atttctattt ttacggcaat aatttacaaa agtaaaatgg
    15001 tgttaaattt taaaatagaa agaactacct ctcaggattt taacttccta aaattagtag
    15061 cagaattgga taaagattta aaaattaaaa atggcactaa gaatgatttc tttgcacaat
    15121 acaataaatc agataccatt aatcatgtta tagtggctta catcaaagaa aaacctgtag
    15181 gctgtggcgc atttaaagag tttgattcag atactgttga gctaaaaaga atgtatgtgg
    15241 tgcctgattt tcgttgtcag ggaattgcgt ctgaaatatt aaatgagttg gaaaaatggg
    15301 catcagagct tcaatataaa aaatgtattc ttgaaaccgg tgacaaaatg aaagaagcta
    15361 ttggacttta tcaaaaacat caatttgtaa aaaccaaaaa ctacggccaa tatgaaaaca
    15421 taacaagtag tttatgtttc aaaaagtcca taggtaataa aaattaataa tacattttaa
    15481 aataataatt ttctaataaa catttacact cataacacaa atgaataaaa caagacttga
    15541 agcattcagc gatggtgttc ttgcaatcat cattaccatc atggttttag aattaaaagt
    15601 accacatgaa acaagtatta aagcactgct cattttatgg ccggtatttt tcagttacct
    15661 catcagtttc atctatgttg gtatctattg gggaaatcat catcatttac tacatacagt
    15721 acatcatgta aacggtaaaa taatctgggc aaatatgggt ttgctctttt gtctatcgtt
    15781 actacccttt acaagtgcct ggatggggga aaatattcct gaaaaaattc cattgatgtt
    15841 atattcaata aatcttgcag tttgtgctgt tgctttttat tttcttcaaa tgtgtgtttt
    15901 accgcaatat aaaaccacaa gtaaactgat tgatgccgta aagaagcaaa aaacaaaagg
    15961 aatgatttcg ttaggtatat atatagcagc agcagtggta tcatttataa tgccatggtt
    16021 aagtataata ctaattatta ttccggcaat actttgggtt attccggata aaaatattga
    16081 agaggcattt gttgaagaac acaaagaata agtttcatca gagataacgc attaaactaa
    16141 acccattgcg ttagtttttc tttttcatcc aaataaatga gtaagaatta attcaactta
    16201 cttattctaa ttacccaaca gcatttatta tgttcttgtg gctgtaatca tcaataattg
    16261 acaacttact gacaaaaatc agtaaatatt aaaagtattt gtttactttt attaaattct
    16321 ttaattactt ttgtgccatt atttaaacaa acacacaata ttatgcaaac agaagttgac
    16381 aacgttttaa acgtaacact actagaacca agaatgaagc accctaccat ttttgcccgc
    16441 tttgatgaac tgaatcccgg agagagttta actattcata acgatcacga ccccaagcct
    16501 ctttactatc agcttttagg tgaacgtggc aacatattta catgggaata tctggagcag
    16561 ggtccgcaat ggtggaaagt aaaaatctca aaacgcataa gtggtgaaag cgatgagaca
    16621 cttggagaaa ttgtagcgaa agacatgcat aaagcacaga ttttcaagaa atatggaatt
    16681 gatttttgct gtggtggtaa gaaaacagtt aaagaagctt gtgaagaaaa aggaattgat
    16741 gttacattag ttgaacaaga attacagaaa gcaggaaaag tagtttctaa cagaccgtta
    16801 ccttataatg attggaatct tgattttctg gcggattaca ttgtgaacac gcatcacact
    16861 tatgtaaaaa acaatcttcc ggaaatcagc ggatatgcag ctaaagttgc aagagtacat
    16921 ggcagtaatc atcctgagtt attaagaata aatcaattgg ttgaagcaat cagctcagag
    16981 ttttcatcac atttagtaaa agaagagcag gtgttgtttc cctatattaa acgtatggta
    17041 gaagcaaaaa acaacaaaga atctgttcaa caatcatcat ttggttcagt aaaagggcca
    17101 ataaatatga tggagattga acatgaatca gcaggtaaaa atatgagcga aataagaaca
    17161 ctcagcaatg attttacttt accggaagat gcctgtgcaa gttacagttt actatttaga
    17221 ttacttgaag aatttgaaga agatttacat acccatgtgc acctggaaaa caacattctt
    17281 tttccaaaag cacttgctct tgaaaaagaa ttagtaacaa agtaaaaaaa atagttgatt
    17341 aatattattt tatagttcat ttaattccgg tagtgtttca ttaaatctga catagacgaa
    17401 aagtcaataa atacagagca gcagaaaggt atggtttgtt atgtttgtga taccagatgc
    17461 agttttcgct ctgtatttat gtcagattaa ttcttactga aaattattct gactttttaa
    17521 tacaaacttt ataaagtcta tttttccaaa atcaaagtat gatgaacact aaattttaaa
    17581 aatcacacaa tggaaaatac tatcaagccg gcacttcaag catggacatc aggaagagtg
    17641 agcggatttt acggcaaaga gttactaaat ctcaacaatg gcaccgtcaa gttagtaaaa
    17701 gtagatcccc atgctaagta tccggaacat atacatcccg acaaaaccga atacgcattt
    17761 gttttagaag gtaatccgga gctttttatt gaaggcagtc attattcagg tcagccgggt
    17821 gaatttttta tttttccatc agcaaaaaaa catgccatac aaaataatac accttcggaa
    17881 tgtacattac tgattggtgc tattacggtt tgaaatattg aatctaatta ttaccttagt
    17941 aaaataatgc acggttaaaa aatgttttcc gatgactata aatgccaata ccaaaatcgc
    18001 aacaatatta aaacagcatc ccgatgcact tgatgtgata gtaagtatca gtcctaagtt
    18061 tgagaaacta cgcaatccgc tattaagaaa aattatggca ggacgaacaa gtctggctat
    18121 ggcatcaaaa ataggtggat gtagtgtaaa tgattttttt gtaaaactgc aaccattagg
    18181 ttttgagatt gacagcacaa ctcaagaaat agagaaagca gcagcaggta aaatgcctga
    18241 gattttcact tcactaaaaa aggaacagat agtttcactt gatgtaagac ctgtgttagc
    18301 tgccggtagt gatccgttaa accttattct tgaaaatgtc aatagcctgg aagccggaaa
    18361 atgtttaaaa ataataaaca catttgagcc cactccactc atcatcatgt tagagaaaaa
    18421 aggttttgta acttatacag acaccattaa tgatgagctc atagaaacct atttctacaa
    18481 aaaatctgac agcaaaaaag aaataaatcc tgtaatcaat acttcgggtg gatgggatga
    18541 aatcatacaa cagtacaaca ataaacttca aactattgat gtacgtgctc tggagatgcc
    18601 gcttcccatg ctaacaattt tagaagcatt agagaaactg ccatctgatc atgcattata
    18661 tgtttatcat aaacgtattc ctgtattttt gctacccgaa ttagctgaga agaaattcga
    18721 ttatcgaata aaagaaattt ctgatggtga agtacacctg cttattttca agtcgtaatg
    18781 aattaaaata tgatcattac tgattctata aaaacaacat cttataaagt ggtgttgcca
    18841 ttttatattt atgctgcatt tgctttttta ctctctgcaa tactactatt aacatcttct
    18901 tccgctttta cagatcatta ttttcagcct cacatcttag ccatcactca tttgatggca
    18961 ttgggttggg gtaccatgat aatattaggt gccagtcatc agcttgtacc tgtattgatt
    19021 gaaggaaaac tttacagcaa tgcattggca tatacttcat ttattcttgc cggaattggt
    19081 attccattat tggtttatgg attttatgta ttcaacatgc acaaccctgc caaatggggt
    19141 ggcaggttta ttttattatc aatcattgcc tacttgatta atcttggtgt aagcatgtcg
    19201 agaagcaaaa aagaaaatat tcacgcttta tttgttttca cctcggtatt gtggttattt
    19261 ctgactggag ctttggggct tgcattagta tataacttta cctacaacat gtttccgcat
    19321 gactctttac attacttacc acttcatgta catgcaggtg taattggctg gtttctgatg
    19381 ctgataatag gcgtaggctc aagactgata cccatgtttt tgatttctaa atatacgaac
    19441 acaaagctac tgaaatggat ttttgtattg ataaacggtg cattaatttc ctatatcttg
    19501 attttttact ttattaaaat cacagaggtt atccttttac cgtggctaat gctttttaca
    19561 ggaattgcac tctttatgta ctattgttat tctgccttca aacatcgcat cagaaaacag
    19621 gttgatgagc caatgaaagt ttcactcttg gctgtaatgc tcatcatgat accattggtg
    19681 ttgttat
//
LOCUS       DAOQNP010000061        19522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_974_length_19522_cov_7.909334, whole genome shotgun sequence.
ACCESSION   DAOQNP010000061 DAOQNP010000000
VERSION     DAOQNP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 19522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19522
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_974_length_19522_cov_7.909334"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKY09_09005"
     CDS             <1..283
                     /locus_tag="PKY09_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13036.1"
                     /translation="YTPDANVNNGIWTDFQTTSSGDLFIHPSANNANRFVGINTTTPQ
                     TTLEVNGTITTKQLIIANENQKQDVTAMLNELKNEIAQLKEQLNQITKN"
     gene            289..1251
                     /locus_tag="PKY09_09010"
     CDS             289..1251
                     /locus_tag="PKY09_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13037.1"
                     /translation="MKKLILLILTFACMFCNAQINLVPNPSFEDTVSCPLVPNQVEKA
                     LYWSSYRPTPDYYNYCSSMQMGMTGVPYNFLGFQYPHSGDAFMGMIPYELTGPTYREY
                     IGVQLLQPTIIGQKYFVTFYVNFNGTIGFTIAINKLGVALSTVSYSDLNPYPITNNPI
                     AYSDSIYTDSLNWSKVQLSFVADSAYNYLILGNFFDDVNTDTLNLGIFNTHSYYYIDD
                     VCLSTDSVYCSGVANFNEVINQDFSSLVKEIKINENNITIDFNKLYHHKSVVIYSYDG
                     KLIYNIKTQLNEINIPTPSSNSIYLIYCTSGNIVYSKKIQILNK"
     gene            1256..3961
                     /locus_tag="PKY09_09015"
     CDS             1256..3961
                     /locus_tag="PKY09_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNB13038.1"
                     /translation="MKTTTNILILLSISNLAFSQVDGLTHTAGGANWYVGWDNTIPSS
                     TVPLVIQHQGQQPIQFWHNDLNLGNTVIRRAEFTVGQSMLENNGTVARTDGLRIWNPG
                     YANSVAYGAEQALDIWTGVGNSTHIRFDGSGLLQSTNNQFEIFGRLNGFWFNAAPLQW
                     QQNNFRSNYVWNIDSIEVGRYSNSNNAQRGFMRWGLQPNPVAPVDAARRVEIYDRDNV
                     PQFRITQTNASIFTDFQTTLNGDLYINPVRNGTYRFVGINTASPQNTLEINSNANYAA
                     GQPGASGLRFTDLRLGSQEGTSNGKVLSVDNNGDVILVRDIGGGSVNSTCTTQDIIAR
                     FDNTGNVIQCSDIYNNAASTSNNRIGFFTTNPRYKFQCEGTSFFTTNQVTPLTPNYGL
                     QHPNSPVIIDSKSNEGLSILHTANGYNKDLMRIYGDNNMQYHIRMVGTPTGMNVANSL
                     TIEHEDNGGNIESWLAFDPGTNTCVMQGNYSGSIPGNHSKLYVEAQNPIPTSSGNNMF
                     DAIRAVNTNTVTVSTTRLTGVNAECVGTRLTAGQPIYNIGGAFKGSGANSSSSSNAFN
                     YGVSGEAFGNNSMVNNYGGFFRSLGTGYSNCGVYSIAQNVSQLSFGIYTSARGGTSVN
                     YGIYALSLSNNCINGNCSSAAGFFNGRVYATNNYYHTSDSTLKQNIVPAPQSDTLLNN
                     LSIYKFNYDTLNNYGLSLEGGTHYGVIAQEVQQVLPQLVKNFLQPAEYDSSGAIISQQ
                     KDILAVNYTEFIPLLIAGYKSQAKEIDTLTSQLTTLQSRLDSLVMAVASCCNAPLLNQ
                     NNSSNQQTIELDNTPAIILGNDPNPFAENTTISWNIPQQDTKTINAMLVFYNQNGSIL
                     KSVKIHQAGAGSLLVYGSNLSSGQYSYSLVVNNKVVKTKQMVKVK"
     gene            complement(4033..6189)
                     /locus_tag="PKY09_09020"
     CDS             complement(4033..6189)
                     /locus_tag="PKY09_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB13039.1"
                     /translation="MIVSQIKKIIFAVAALFIATASFAQQDDLVLADQLYSKYSFGKA
                     IPLYEKILSKNYNHGTASKLADCYRKNNQYKEAEKWYERITADTNATNNEHLQYAKVL
                     ISLDKKDEAEMHLEKYLAKNPNDKLVAEQLNALKNNTFGEQQGSWIVKRLLINSVNGD
                     MCAVPYEDGIVFASSREDGKSKTQDWTSKPYLSLWFSKGKENNFSTPVLLELADKSKY
                     NDGPVSFSSDGNEMYITRNYEENGKIVRDQNRIVRLKILSATKANGKFDAFKEFKYNN
                     KTYSCAHPFISKDGNKLYFASDMPGGKGGMDIWVCNRVNGDWDKPVNLGAEVNTTENE
                     VFPYIDHEGSLFFSSNGRSGMGGLDIFKCEVENGKFKTAENIGSPLNSTYDDFAYVYD
                     KKNMCGYLTSNRDTKNENDDLYMFKKNCVPLDGLVYDKETNLPLANAKVVINEGGNKF
                     AEVQTDDKGKFKSCLSIGQNYDFIASKDEYRENKVSINGVNDEPQVLKIPLSKIPAFT
                     LEGHVYLEEDKSSLPNHPVKLMNLKTNKETETLTDANGNYHFDLEPETNYKVMTFREN
                     CADNFADRSTIGLTKSTVLKADFGFFCQGDIIRVDNIYYDLAKWNIRPDAAKELDKLV
                     DIMKKYPKMQIELGSHTDCRASYKYNMDLSEKRAKSAVQYIVKKGVEASRMTYKGYGE
                     SKLINHCECEGTRVVPCTEEEHQQNRRTEFKVLNVK"
     gene            complement(6210..7199)
                     /locus_tag="PKY09_09025"
     CDS             complement(6210..7199)
                     /locus_tag="PKY09_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNB13040.1"
                     /translation="MKETINRIHNNKIRKAMYSSIKKIWIMSVLLTSICSIKANAQYD
                     PLFTQYMFNEMFINPAYAGSRDQISATALYRNQWVGMEGAPKTQTVSLHGPVKNKKVG
                     LGISFMNESIGVEKKVGFFTNYAYRLQTSENGRLAFGLQAGLIHMKENYLDVITHDQN
                     DIQFSQNVSKLVPNAGFGIYYNTKRFYAGFSIPRLIENYIDSVNGGDIKNRFTHSNFH
                     YFLTGGYVFDVSPEIKLKPMTMIKASDGATIQADVTLAAYFNEIFWIGLTYRTGAAAS
                     VFAQVNLTKQLRLGYSYDYTTNQLNKYNSGTHEITLGYDIPLKRTGVLSPRFF"
     gene            complement(7204..19101)
                     /locus_tag="PKY09_09030"
     CDS             complement(7204..19101)
                     /locus_tag="PKY09_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB13041.1"
                     /translation="MIQKFQLKLMTASLLFTLFLIVSHDKTYADGSPQFKPDTTKATN
                     LMILNTQTTYGTFAGYSATDEDRLYVRINNPAQEKIYFGIGQRSTNSDWYVRIKDPNG
                     NIIFGPQKLPTGNAQGFITYHSQAIAGPNVVNAAGYNGYVCNPTAGLPGAYYIEFNKG
                     SGTSVAGNTELGLGIFDVTVVNTSNNTKVTGRLFSKNWGFNTESYSNPFFGSFFIYGQ
                     DSSVTKVDLNGIKPFKFRVSCNRFGTSNSGNATIDRRSKTGFAVPVEYRLFLTDPDII
                     AYPNGSMSFLTGPPTLERCVRDSICINVGLIKTSDVTVVIDRNNNNKFDAGTADRKLF
                     FPQVPSGSSCLYWDGKDGLGNLIPVGNPVSVILSIESGLVNLPMYDVENHETGFSMSV
                     IRPGSAQFVDSLFYDDVLVGGLTNLGGCPSPCHTWTSSPANSDQNTIGESNTINTWWF
                     AHKLSSKTIINLPDYLVNSAGTDKNICTKGGGQDSIAFNGTIAYSQSSFTGAVQWTTS
                     GTGQFSPSDTVKNGYYIPSAADINAGSVMLIMGPKNVCATVKDTIIINLRKTPQVSLT
                     GTNINCFGQQTGGITTTVSNSVAPYTYSWSNGASSQNLSNAGAGTYTVTVTSSQNCST
                     TATATLTQPAAALSATASATGVGCYGGATGAINLTVNGGTSPYNFTWSNGASTQNVTG
                     LTAGNYTVTVTDSKGCTSTQSSISITQPAAALSLANSKVNVNCFGGNNGSISVTASGG
                     TTPYTYLWNTGATSSSVTGLTAGNYTVTVTDANSCTTSQTSINISQPAAALAATPTAV
                     NVNCFGNATGEINLSVSGGTAPYSYSWSNGATSQNISNIAAGNYTVTVTDSKGCTSTQ
                     SSIAVTQPSASLTSSANTVAVDCFGNATGSINVTVNGGTSPYSYSWNTGATSQNLSNI
                     TSGVYSVTITDANGCTSSQNSISVAQPSAALTTSATGTNVNCFGNATGAVSLTVNGGT
                     GPYAYTWSNGATTQNISNIVAGNYSVTVTDANGCTSSQNAISITQPSAALNSSVASTQ
                     NINCYGNGTGSINIAVSGGTSPYSFNWSNGATTQNLANLTSGTYSVTITDAKGCTSTT
                     NNITLTQPSATISANIQSMQNVSCNGGGNGALNLTVTGGTAPYTYLWSNGATTQNING
                     LSSGIYNVTITDSKGCELQATGTVSQPANALSASISSSQNVLCFGGNNAHINLSVSGG
                     TSPYSFNWSNGATTQNISNITAGNYTVTVTDDNGCIYTINKNISQPSGALTASTSSST
                     EVSCYGLNNGSINVGVTGGTSPFTFVWSTGATTQNLTGLIAGSYTVTVTDANGCTTTL
                     NKTINQPSAALAASLESSEEVSCNGGSNGQININVNGGTSPYSYNWSNGATTQNIQNL
                     TSGTYTVTVTDANNCTTTLTKVIGQPAQALNASIAATQHVNCFGGNNASISITVGGGT
                     TPYSYNWSNGATSQNISGLAAGSYSVTITDNNGCTTNAIQTITQPAQALSASVTASQN
                     ISCYNVPNGSMSVTASGGTSAYTYLWNNGATTASISGLAAGSYTVTITDANGCTTTAD
                     KTITQPSAQLDATISASTNVSCYGLSNGSVSINVNGGTAPYTYAWSNGATSQNITGLS
                     SGAYTVTVTDNNGCTTSETTTVSQPAQALNASVSSSQNVNCFGGNNAAIQLSVSGGTT
                     PYSYNWSNGATTQNISGITSGTYTVTVTDVNNCSTTLTKAITQPSAAMAASVSTSQNV
                     SCYGQNNGNINLAVSGGTAPYSYSWSNGATTQNINDLTAGSYSVTVTDANGCTAQVMA
                     ITISQPAAALATSINTTDVLCFGLSTGAISTTVSGGTAPYTYIWNTGATTSGLSNVNS
                     GTYSVTVTDAQGCTTSQAAIQVNQPAAILDANVATNVSVLCYGGNNGSLTLAVSGGTA
                     PYTYLWSNGATTQNITSITAGTYSVTITDANNCGKAISGVLVNQPDNPLTTSVASSQN
                     VACNGGGNGSITLNVAGGTLPYTYNWSNGATTQNISGLYAGTYNVSITDANGCTAAAT
                     GVVNQPSGALTSSVASSQDILCYGGNNGSVTLSVVGGTAPYTYSWSNGATTQNLTNLT
                     AGNYAVTVTDANNCISQSSVVIAQPSGALAASISASQNILCYNQSTGSIDVSVTGGTA
                     PFTYNWSNGATSQDITNITAGTYSVSITDANGCTTQMNGIVLTQPSGALAVTPQLTAV
                     NCYDNYTGAITLNVNGGTAPYSYNWSNGATTQNLSNIAAGTYSVTVTDANGCTSASTS
                     LQITQPTAALNNTYTSQNINCFNVNTGSISVNVTGGTAPYSYNWSNGASSQNLSNLAA
                     GTYTLTITDANGCSSTTGSVSITQPAAALAYTFTTSDAGCFGNNTGGIDLTVTGGTTP
                     YSFSWSNGATTEDISGIASGTYVVTITDGNGCTNTSASITVSQPAGALNSSVSSSQNI
                     NCFGNQTGSITLSVNGGTAPYSYNWSNGATSQNISNIGAGTYSVTITDANGCSNLLSG
                     IALTEPTGALTSAISGTQNVNCYGQSTGNITLNVNGGTAPYSYNWSNGAITQNLSSLS
                     AGTYSVTITDANNCTSSINNIIISEPAAVLFGSVNSVVNVNCTGGNNGSISLNVSGGT
                     PPFSYNWSNGAITQNISGLTSGSYAVTITDANGCTFSLSEIITQPSSSLTSAITSSQN
                     INCFGNATGSIDLSANGGTTPYNYNWSNGATSEDQNGIAAGTYTVTITDANGCTSSQS
                     ATLTQPSASLTSSINNLTNVGCFGQSTAAILINVNGGTTPYSYLWSDGSTNQNLIGVG
                     AGNYSVNITDANGCTTSIAGIAISQPAAGLGATVTSNQNVSCTGGGNGSIDITVNGGT
                     MPYSFNWSNGAVSEDISNLGTGAYTVTITDANGCTFTLTENVGQPASNLATSIQSSQN
                     VDCYSNATGAIDLNASGGTAPYTFAWSNGATTEDISGLTSGVYTVTVTDANGCYNNQT
                     VTISQPAGVLASSITGMQNILCFGNATGNIDLTITGGTTPYNFNWSNGANTEDLSNIA
                     AGTYSVTVTDANNCSVQQNITLTQPAAALTSSITSTSPVLCFGGNTGSANLDIQGGTT
                     PYSIVWSNGSTTQNQPSLAAGSYNVTITDANGCTSNQSVTINQPQDSISGGISSIGNI
                     GCYGNATGNIDLTVNGGTTPYSYLWNSGQTTQDLNNITSGLYEVTVTDQNGCTWSISA
                     NLTQPALALSNNLSLQQNVNCFGGNNGAINTNPVGGTSPYTYFWSNGNNTQNISNLAS
                     GWYSVTVTDANGCVATDSIEVQQPAAAVAATYQMTSSVSCFGGNNGAVDVTVTGGTMP
                     YSYVWNTGDVTEDLSNLTAGNYTVSITDANGCTFSLVTTVTQPNGPLSPSIVMTPVGC
                     FADSTGSINLTVTGGTPPYQYYWSNGDTTANLTNLTGGSYSVYIVDSNSCIAQTSTYV
                     PTPEGALYAFPVVTNLNCSSLTSGSIILNPTGGTVPYSYSWSNGDTTENLVNIGAGQY
                     TVTITDSAGCTYSQNFTINGPTQALALTGQQTNINCHGNNTGSASVTASGGIPPYSYI
                     WTNGMTTPSISNLAAGNYKVIVTDSYNCSTDTVFVITQPDSVLSNIISATQIPCYGLN
                     NGSISVTTQGGTPAYTYQWSNGSSSSQLSNLTPGTYTLTVTDQKGCNDQQTVTITQPA
                     FALTASSTANEANCLTGNGANIDLTVAGGTQPYAYVWSNGSTYQSLTNVGAGTYTVSI
                     TDANGCTVQQTQIVTDISVFNMNADGPTEFCVGGKVTLNATVVQNGTYQWYVNGQILP
                     GATSPSLTTPAAGIYTVSLTNSCGNFSSTPVEVKVNSVSGYTVSPGMMICPSNGEIAQ
                     LFATGGSWYSWHPGAGLNDSTISNPIATPKASTTYTVTIRSNEGCEVVSEVMVSVICD
                     TLFVPTGFSPDGNAVNDTYVIDGLSKYPGNLLYIYNRWGNLVYKKRDYDNSWDGTSNV
                     AGVYIGQQLPNGTYYFILDLNDGKKPMQGYLTLKR"
     gene            19308..>19522
                     /locus_tag="PKY09_09035"
     CDS             19308..>19522
                     /locus_tag="PKY09_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13042.1"
                     /translation="MKKVIFSLFFVIAALAVKAQTIIGKVDVDGVPQFTAAIADCKSE
                     LQKVTSSKGTFSTMKLQDITFSQMSKG"
BASE COUNT         5583 a         3707 c         3933 g         6299 t
ORIGIN      
        1 ctacacaccc gatgccaatg taaacaacgg catctggacc gattttcaaa ctacctctag
       61 tggggatttg tttattcatc cctcagcaaa taacgcaaac aggtttgtag gtataaacac
      121 caccacaccc caaaccaccc ttgaagtaaa cggcaccata accaccaaac aactcatcat
      181 agcaaacgaa aaccaaaagc aagatgtaac ggccatgctc aacgaactaa aaaatgaaat
      241 agcccaacta aaagaacagt taaatcaaat aacaaaaaat taaagttgat gaaaaaactt
      301 atactactta ttttaacttt tgcttgcatg ttttgcaatg ctcaaattaa tttagtgcct
      361 aacccgagtt ttgaggatac ggtaagttgc ccattagtgc ctaatcaagt tgagaaagca
      421 ctctactgga gctcttaccg tcctacaccg gattattata attattgcag tagtatgcaa
      481 atgggaatga caggtgttcc atataatttt ttgggattcc aatatccaca ttctggagat
      541 gcttttatgg gaatgatacc ttatgaatta acaggaccaa cttaccgaga gtatattgga
      601 gttcaattat tgcaaccaac aataattggc cagaaatatt ttgttacctt ttatgttaat
      661 tttaatggaa caataggttt tactattgca ataaataagc tcggtgtcgc tttatcaaca
      721 gtgtcttatt ctgatctaaa cccatatccg attacaaata atcctattgc ctattccgac
      781 tcaatttata cagactcgct aaattggtca aaggttcaat tgtcttttgt agctgactct
      841 gcttataact atctgattct aggtaatttt tttgatgatg taaatacaga cacactgaat
      901 cttggtattt ttaatactca ttcatactat tatatagatg atgtttgttt atccactgat
      961 tctgtttatt gttctggcgt tgcaaatttc aacgaagtaa ttaatcagga tttttcatct
     1021 ctcgtaaaag aaataaaaat aaacgaaaac aatataacta ttgattttaa taagttgtat
     1081 catcacaaat ctgttgtaat ttattcttat gatgggaaat taatttacaa tataaaaact
     1141 caattaaatg aaattaatat accaacgccc tcatcaaact ctatttattt gatttattgt
     1201 acatcaggga atatagttta ttcaaaaaaa attcaaatat taaacaaata aatttatgaa
     1261 aacaacaaca aacattctaa ttcttttgtc aatctctaat ttggcatttt cacaagtaga
     1321 tggactaacc catacggctg gaggtgctaa ctggtatgtg gggtgggata acacaattcc
     1381 ttccagtaca gttccgttag tcatccagca ccaaggtcag cagccaatac aattttggca
     1441 taatgatttg aatctgggaa atactgtaat aagaagagca gaatttactg taggtcaatc
     1501 tatgcttgag aataatggaa cagttgcacg aactgatggc ttaaggattt ggaatccagg
     1561 atatgctaac tctgttgctt atggtgctga acaagcattg gatatttgga caggcgttgg
     1621 caacagtact catatacgtt ttgatgggag tggattgctt caatctacca ataatcaatt
     1681 tgaaattttt ggcagattaa atggattttg gtttaatgct gcaccattac aatggcagca
     1741 aaataacttt aggtcaaact atgtttggaa tatagacagc atagaggtgg gaaggtattc
     1801 aaacagcaat aatgctcaaa gaggttttat gcgatggggc ttgcagccaa atcctgttgc
     1861 ccctgttgat gctgcaagaa gagtagaaat ttatgataga gataatgtgc cacagtttag
     1921 aattactcaa acaaatgctt ctatttttac tgattttcaa actacattaa atggtgattt
     1981 gtacatcaat cccgttagaa acggaaccta tcgttttgta ggaataaaca cagcaagccc
     2041 acaaaataca cttgaaataa attctaatgc aaactatgct gccggtcaac cgggagcaag
     2101 cggcttgcgg tttacggatt tgcgcttagg ctcacaagaa ggtacttcta atggtaaagt
     2161 acttagtgtt gacaataatg gcgatgttat tttggtgagg gatattggtg gcggtagtgt
     2221 caacagcacc tgcaccacac aggatattat agcccggttt gacaatacgg gaaatgttat
     2281 tcaatgcagc gatatttaca acaatgcagc ttctacttct aataacagaa taggtttttt
     2341 tactaccaat ccacgttata aatttcaatg tgaggggaca tcttttttta caacaaacca
     2401 agttacacca cttactccaa actatggcct acaacaccca aattcacctg ttataattga
     2461 ctcaaaaagt aatgaaggcc tttctatttt acacacagct aatggatata ataaagattt
     2521 aatgcgaatt tatggtgata acaatatgca atatcatatt cgaatggtag gcacacccac
     2581 aggaatgaat gtagccaaca gcttaacaat agaacatgag gataacggtg gaaacattga
     2641 aagttggtta gcttttgatc ccggtacaaa cacttgtgta atgcaaggaa attatagtgg
     2701 ttctataccc ggcaatcatt caaaattata tgtggaggcg cagaacccaa tcccgacctc
     2761 ctcaggaaac aacatgtttg atgcaataag agcggttaac acaaatactg tcactgtttc
     2821 aacaacaagg ttaacaggag taaatgccga atgtgttgga acacgcttga ctgcaggtca
     2881 acctatttat aatattggtg gggcgttcaa gggttcggga gcaaattcat cctcttcatc
     2941 taatgcgttt aattatggtg tttcaggaga agcttttgga aacaattcaa tggttaataa
     3001 ctatggcgga ttttttcgaa gtttaggaac aggttatagc aactgtggtg tttatagtat
     3061 agcacaaaat gtaagtcagc ttagctttgg tatttatact tcagcaagag gtggtacttc
     3121 agttaattat ggcatttacg cactttcact tagcaataat tgtattaatg gaaattgctc
     3181 aagtgcagca ggatttttta atggaagagt ttacgccacc aataattatt accataccag
     3241 tgattctaca ttaaaacaga acattgttcc tgcaccacaa agcgatactt tgctcaacaa
     3301 tttatcaatt tataaattta attacgacac actcaataat tatggtcttt cattagaagg
     3361 cggtactcat tatggtgtaa ttgcacagga agtacaacaa gtattgccac aattagtaaa
     3421 gaatttcttg cagcctgccg agtatgattc atcaggtgca ataatcagtc aacagaaaga
     3481 tattcttgca gtaaactaca cggagtttat accgctgctg atagcaggat ataaaagtca
     3541 ggctaaggag atagacacct taaccagcca attaactaca ttgcaaagca ggctcgattc
     3601 gttggttatg gcggtagcct catgctgcaa tgcgcccttg ttaaatcaaa acaatagcag
     3661 caatcaacaa accatagagc tggataacac accggcaatc atattgggca atgaccccaa
     3721 tccatttgca gaaaacacca ccatcagttg gaacattcca caacaggata caaaaacaat
     3781 aaatgccatg cttgtttttt acaatcagaa cggcagcata ttaaagtcgg ttaaaataca
     3841 tcaggcagga gcaggctcgc tgttggttta tggcagcaat ctttcatcgg gtcaatacag
     3901 ctattcgttg gtggtcaata ataaagtcgt taaaaccaaa caaatggtaa aggtgaaata
     3961 gtgcgtttta aaaacaaacg agggcggctg cattcgcagc cgccctcgtt ttgttttatt
     4021 actgttttca ccttacttca cattcagtac cttaaattct gttcgtctgt tttgctgatg
     4081 ttcttcttct gtacaaggca caacacgtgt tccctcacat tcgcaatgat taatcagctt
     4141 tgattctcca taacctttgt aagtcattct tgaagcctca acaccttttt tgacaatgta
     4201 ttgtacagct gacttagcac gtttttctga aagatccatg ttgtatttat agctggcgcg
     4261 gcagtcggtg tgtgaaccta attcgatttg catcttagga tattttttca tgatgtcaac
     4321 caacttgtcc aattcttttg cagcatcggg tctgatattc cacttagcca aatcgtaata
     4381 aatattgtcc acgcgaatta tatcaccctg gcagaagaaa ccaaagtctg ctttcagaac
     4441 ggttgacttc gttaatccaa ttgtagaacg gtcagcaaag ttgtcagcac aattttctct
     4501 gaaggtcatt accttgtaat tggtttccgg ttctaaatca aaatgataat ttccatttgc
     4561 atcagtcagc gtttcagttt ctttatttgt ttttaaattc atcagtttaa ccgggtggtt
     4621 aggtaatgaa gatttgtctt cttccaaata tacatgacct tcaagagtaa atgcaggaat
     4681 ttttgataat ggaattttaa gtacttgtgg ttcatcattc actccgttga tagatacttt
     4741 gttttctcgg tattcatcct tcgatgcaat gaaatcataa ttctgtccga ttgatagaca
     4801 tgatttgaat tttcctttgt cgtcagtttg aacttcggca aacttattgc ctccttcatt
     4861 gattacaact ttagcattgg caagtggcag gttcgtttct ttatcataaa ccaaaccatc
     4921 taaaggcacg cagttctttt tgaacatgta caaatcgtca ttctcatttt tagtatctcg
     4981 gtttgatgtc aagtagccac acatgttttt cttatcataa acataggcaa agtcatcata
     5041 agttgaattg agtggactac ctatgttctc tgctgttttg aattttccat tttcaacttc
     5101 acatttaaaa atgtctagtc cgcccatgcc actacgtccg tttgaagaga agaacaggct
     5161 accctcatga tcaatatatg gaaatacttc gttctctgtt gtgtttactt ccgcaccaag
     5221 atttacgggc ttatcccagt cgccattaac tctgttgcaa acccaaatat ccatgccgcc
     5281 ttttccaccg ggcatatcac tggcaaaata aagtttgttg ccatctttag agataaatgg
     5341 atgagcacat gaataagttt tgttgttgta tttaaattct ttaaatgcat caaactttcc
     5401 gttagccttt gttgcactta atatttttaa tctgactatt ctgttctgat cacgtacaat
     5461 ttttccattc tcttcatagt ttcgggtaat gtacatttcg ttaccatcag aagagaatga
     5521 aacaggtccg tcattatatt ttgatttgtc ggcaagttcc agcagcaccg gagtgctgaa
     5581 attattttcc ttacccttac tgaaccataa agaaagatat ggttttgacg tccagtcctg
     5641 tgttttgctt ttaccatctt cgcgagatga tgcaaagacg attccgtctt cataaggtac
     5701 agcgcacatg tcaccattaa cagagttaat aagtaaacgt ttaactatcc atgaaccctg
     5761 ctgctcgcca aaagtgttat tctttaaagc atttagctgc tctgccacca atttgtcatt
     5821 tggatttttt gctaaatatt tttcaagatg catttctgct tcgtcttttt tatcaagact
     5881 tatcagcact ttggcatatt gaagatgttc gttattggtt gcattggtgt ctgcggttat
     5941 tctctcatac cacttttctg cttctttata ttgattgttc tttctgtagc agtcggcaag
     6001 ctttgatgct gtgccatgat tgtaattttt agagagaatt ttctcataca gtggtattgc
     6061 tttaccaaag ctgtacttgg aataaagctg atctgcaaga accaaatcat cctgctgcgc
     6121 aaagcttgct gttgcaatga aaagtgctgc aacggcaaaa attattttct ttatctgtga
     6181 tactatcata attcagttta ttaatttggt tagaagaagc gtggactaag tacacctgtg
     6241 cgttttagtg gaatatcata gccaagtgtt atttcatgcg tgcccgagtt gtatttgttc
     6301 aactggtttg ttgtgtaatc ataagagtag ccaagccgta gctgtttggt taagttaacc
     6361 tgcgcaaata ctgatgctgc tgcacctgtt ctgtatgtta atccaatcca gaaaatctca
     6421 ttgaagtatg ctgcaagagt aacatctgcc tgaatagttg caccatcaga tgcttttatc
     6481 atggtcatag gtttcaactt tatttccgga ctcacatcaa acacatagcc tccggtaagg
     6541 aagtagtgaa aattactatg tgtgaaacga ttttttatat caccaccatt gactgagtca
     6601 atgtagtttt caattaatct cggaatggag aagcctgcat agaatcgctt ggtgttgtag
     6661 tatattccga agcctgcatt aggcaccaac ttcgaaacat tttgactgaa ctgaatatcg
     6721 ttctggtcgt gtgtgataac atcaagatag ttttctttca tgtggattag gcctgcctgc
     6781 aatccaaacg ctaagcggcc attctcactg gtttgcagtc tgtatgcata gttagtaaag
     6841 aaacctactt tcttttctac acctatagat tcattcatga aagaaattcc taaacctacc
     6901 tttttgtttt tgacaggacc atgtaatgaa acggtctgtg tttttggagc accttccata
     6961 ccaacccatt gatttctgta caatgcagtt gctgatatct gatcgcgcga gcctgcatat
     7021 gccggattga taaacatttc gttaaacatg tattgtgtaa acaatggatc gtactgtgca
     7081 ttggctttga tgctgcagat agaggtcagt aatactgaca taatccatat tttttttatt
     7141 gatgaataca ttgcttttct tattttgttg ttgtgaattc tgtttattgt ttctttcata
     7201 ttcttatcgt ttaagtgtca gatacccttg cattggcttc ttgccatcat tcaggtcgag
     7261 tatgaaatag tatgttccgt tgggtaattg ctgtccgata taaactccgg caacgtttga
     7321 agtaccgtcc cacgaattgt catagtcacg ttttttgtaa actaagttac cccatctgtt
     7381 gtagatataa agtaagttac cgggatattt acttagtcca tcaataacgt aagtatcgtt
     7441 tactgcatta ccatccggtg agaaacctgt tggcacaaac aatgtgtcgc agataacact
     7501 taccattact tcgctcacca cttcgcaacc ttcgttggaa cgaatggtaa cagtataggt
     7561 tgttgaagct tttggtgttg caataggatt ggaaatagta gagtcattca accctgctcc
     7621 cggatgccag ctgtaccatg aaccaccggt tgcaaaaagt tgtgctatct ctccattgct
     7681 cggacaaatc atcatgcccg gactgactgt gtacccactc actgaattta ctttcacttc
     7741 aacaggtgta gaactaaagt tgccgcatga gttggttaac gatactgtat atatacctgc
     7801 agcaggagta gtgagtgatg gtgatgttgc tccgggcaat atttgtccgt taacatacca
     7861 ttgataagta ccattctgaa ccactgttgc atttaaagtt acttttccgc caacgcaaaa
     7921 ctcagttggt ccatcagcat tcatattgaa tacggagatg tcggtgacta tctgtgtttg
     7981 ttgtacagta caaccgttgg catcagtaat gctgacagtg tatgttcctg ctccaacatt
     8041 ggtaaggctt tggtaagttg aaccattgct ccaaacataa gcatatggtt gtgtaccacc
     8101 ggcaacagta aggtcaatgt tagcaccatt acctgtaaga cagttagctt cattagctgt
     8161 tgaagatgca gtgagtgcaa atgcaggttg tgttattgtt acagtctgct gatcgttaca
     8221 tcctttctga tcggtaacag ttaatgtata tgttcccgga gtaaggttgc tcaattgaga
     8281 actgcttgag ccattgctcc actgataagt gtaagcagga gtaccacctt gtgtagttac
     8341 actaatgctt ccattgttta gtccgtaaca tggtatctgt gttgctgaaa ttatattact
     8401 taaaactgaa tccggttgtg taattacaaa caccgtgtct gttgaacagt tgtaactgtc
     8461 ggtaacgatt actttgtaat tgccagcagc gagattgctt attgaaggtg tagtcattcc
     8521 gttagtccag atgtaggagt aaggaggaat accacctgat gcagttactg aagcagagcc
     8581 tgtgttatta ccatgacaat taatattggt ttgttgtcct gttaatgcta aagcctgtgt
     8641 aggaccattg atagtaaagt tttgtgaata tgtacatcct gcactatctg taatggttac
     8701 agtgtattgt cctgcaccaa tgttaaccag attttctgtc gtgtcaccat tgctccaact
     8761 ataagaatag ggtactgttc ctcctgttgg attcagaatt attgaacctg atgtcagaga
     8821 tgaacagtta aggtttgtaa caacagggaa ggcatagagt gcaccttcag gcgtaggaac
     8881 ataggttgag gtctgagcaa tacagctgtt agagtcaaca atatatacag agtatgaacc
     8941 acctgtcaga ttagttaggt tagcagtagt atcaccattg ctccaatagt actgataggg
     9001 tggtgttcca ccggtaactg taagatttat tgagcctgta ctgtcggcaa aacaccctac
     9061 cggagtcata actatacttg gagacagtgg gccattaggt tgtgttactg ttgttacaag
     9121 actgaaagta caaccatttg catcagtgat tgaaacagta tagttacctg ctgttaaatt
     9181 cgataagtct tctgtaacat caccggtatt ccatacataa ctgtaaggca ttgtgccacc
     9241 agttacagta acatctacag caccgttatt tccaccgaaa caacttacag aagatgtcat
     9301 ctgataggtt gcagctacag cagcagcagg ctgttgtact tctattgagt cagttgcaac
     9361 acatccgttt gcatcagtca cggtaacaga ataccatcct gaagcaagat tgctgatatt
     9421 ttgtgtgttg tttccattac tccagaagta agtgtaagga cttgttccgc caacaggatt
     9481 agtgttaata gcaccattgt tgccaccaaa acaatttaca ttttgttgta gtgacagatt
     9541 gttgcttaat gctaaagctg gttgtgtaag atttgcagaa attgaccatg tacaaccatt
     9601 ttgatctgta acagtaactt catataaacc ggaagtaata ttgttaagat cctgtgttgt
     9661 ctgacctgag ttccacagat atgaatatgg cgtagtacca ccattaacag taagatcaat
     9721 atttccggtt gcattaccat agcagccgat gttgccaata gaggatattc cacctgaaat
     9781 actatcttgt ggctgattga tagttactga ctgatttgat gtacagccat tagcatcggt
     9841 aatagttaca ttataacttc ctgcagctaa tgatggttga ttttgtgtag ttgaaccatt
     9901 gctccataca attgaataag gagttgttcc accttgtata tcaagatttg cacttcccgt
     9961 atttccacca aagcaaagta ctggagacgt agatgtaata gatgaagtga gagcagcagc
    10021 aggttgtgtt aaagttatgt tttgttgaac cgaacagttg tttgcatctg taacagttac
    10081 actgtatgtg cctgcggcaa tgtttgataa atcttcagtg tttgcaccat tgctccagtt
    10141 aaaattgtaa ggagttgtgc cgcctgttat tgtaagatct atatttccag ttgcattacc
    10201 aaagcaaagt atgttctgca tgccggttat gcttgaagct aagactcctg ccggttgtga
    10261 aatggttaca gtttgattgt tataacatcc attggcatca gttacagtta ctgtataaac
    10321 accggaagta agtcctgaaa tatcttcagt agttgctcca ttgctccatg caaatgtgta
    10381 tggtgctgtt cctcctgatg cattcaggtc aatagctcct gttgcgttgc tatagcaatc
    10441 aacattttgc gaagactgaa ttgaagttgc aaggttcgaa gcaggctgac ctacattttc
    10501 tgtcagagta aatgtacaac cattggcatc cgtaatagtt acagtgtatg ctcctgttcc
    10561 aagatttgaa atgtcttcac ttactgctcc attactccag ttgaatgagt atggcattgt
    10621 tccaccattt actgtaatat caatagaacc gttaccacca cctgtgcatg atacattttg
    10681 attcgatgta actgttgcgc ccaatcctgc agcgggttga cttattgcaa ttcctgcaat
    10741 ggatgtagta caaccgtttg catcagtaat gtttacacta tagtttcctg caccaacacc
    10801 aatcaggttc tgatttgtgc tgccatcact ccacaaatag gagtaaggag tagtgccacc
    10861 attgacatta attaatattg ctgctgtact ctgaccaaaa caaccaacat tagttaagtt
    10921 gttgatgctt gaagtcagtg atgctgatgg ctgtgttaat gtagctgatt gtgatgatgt
    10981 acaaccattg gcatcagtga tggttactgt ataagttcct gctgctatgc cattctgatc
    11041 ttctgatgta gcaccattgc tccagttgta attgtaagga gtagtacctc cgttggctga
    11101 caagtcaatt gatcctgttg cattgccaaa gcagttaata ttctgtgatg aagttattgc
    11161 tgaagttaat gagctcgagg gttgtgtgat aatttcagaa agtgagaatg tacatccgtt
    11221 ggcatcagta attgtaacag catagctgcc tgatgtaagt cctgatatgt tttgtgttat
    11281 tgcaccattg ctccagttat agctgaatgg tggtgttcca ccacttacat taagacttat
    11341 tgatccgtta ttaccgcctg tacaattaac attcacaacg ctgtttacac tgccaaagag
    11401 tactgcagca ggttcgctga tgatgatatt attaattgat gatgtacagt tgttggcatc
    11461 tgtaatggta actgagtatg tgcctgcaga tagactgcta aggttttgag taattgcacc
    11521 attgctccag ttgtaggagt atggtgctgt tccaccgttt acattaagtg tgatgtttcc
    11581 tgtactttgt ccgtagcagt taacattttg agttcctgaa attgctgaag tcaatgcacc
    11641 tgtaggttct gttaatgcaa ttcctgaaag taaattgctg catccgttgg catcagtaat
    11701 tgttactgaa taggtgccgg caccaatgtt agaaatattt tgtgaagtag caccattgct
    11761 ccagttgtat gaatacggtg ctgttccgcc atttacagaa agtgtaatag atcctgtttg
    11821 atttccgaag cagttgatat tttgtgaaga agaaacgctt gagtttaatg cacctgcagg
    11881 ttgacttact gtgatacttg ctgacgtgtt agtgcaacca ttaccatctg taatagtaac
    11941 tacataagtg ccagatgcta tgcctgaaat atcttcagtg gtagcaccat tactccaact
    12001 gaaagaataa ggagtggttc caccggtaac tgttaagtca atgccacctg tgttgttgcc
    12061 aaagcaacct gcatcacttg ttgtgaatgt ataagctaat gcagcagcag gttgcgttat
    12121 tgatacgctg cctgttgttg aactacatcc attggcatca gtaattgtta gtgtgtaggt
    12181 tcctgcagca agattgctaa ggttttgtga tgatgcacca ttgctccagt tgtagctgta
    12241 tggtgcagtg ccacctgtta cgtttacaga aatacttcct gtatttacat taaagcagtt
    12301 gatgttttgt gatgtgtaag tattgttaag agctgctgta ggttgtgtta tttgcagcga
    12361 agttgaagct gaagtacatc cgttggcatc tgtaacagtt acactgtaag tgccggcagc
    12421 aatattactt aagttttgtg ttgttgcacc attgctccag ttgtaggaat aaggtgctgt
    12481 accaccgttt acattaagtg tgatagcacc ggtataatta tcataacagt ttacggctgt
    12541 taattgtggc gttacagcta atgcaccgct tggctgggta agtacaattc cattcatctg
    12601 agttgtacaa ccattagcat ctgtaatact aacactataa gtgcctgctg taatattggt
    12661 aatatcctgt gatgttgcac cattgctcca gttgtaggtg aagggtgctg ttcctccggt
    12721 tacactcaca tcaatacttc ctgtactttg attgtagcaa agaatatttt gtgaagctga
    12781 aatgcttgct gccaatgcac ctgatggctg tgcaattacc acggatgact gactgatgca
    12841 attatttgca tctgtaacag taactgcata attacctgct gtcaggtttg ttaagttttg
    12901 tgtagtagca ccattgctcc aactataagt gtatggtgct gttccgccaa caactgataa
    12961 tgttactgaa ccattgttgc caccataaca aagaatatcc tgagatgaag ccactgatga
    13021 agttaatgcc ccggaaggct gattaactac acctgtagca gcagcagtac agccattagc
    13081 atcagtaatt gatacattat aagttcctgc atacagtcct gaaatatttt gagttgttgc
    13141 accattactc cagttgtagg tatatggcaa agtaccaccc gcaacattaa gcgttattga
    13201 accatttcct cctccattac aagcaacgtt ttgtgatgaa gctacacttg tagttaatgg
    13261 gttgtctggt tgattaacca aaacaccgga tatggccttg ccgcagttgt tggcatcagt
    13321 tattgtaact gaataagttc ctgcagtaat acttgtaata ttttgagttg ttgcaccatt
    13381 actccataag taagtgtatg gtgctgttcc accgcttaca gcaagtgtca gacttccgtt
    13441 gttgcctcca taacaaagaa cagaaacgtt agtagcaaca ttagcatcta aaattgcagc
    13501 aggttgattt acctgtattg cagcctggct tgttgtacag ccttgtgcat cagtaactgt
    13561 tacactatat gttcctgaat ttacattaga taaacccgaa gttgttgcac cggtattcca
    13621 gatgtatgta taaggagcag taccgcctga tactgttgtt gagattgcac cggttgatag
    13681 gccaaaacat aaaacgtctg tagtgtttat agatgtggct aaggctgctg ccggttgcga
    13741 tattgttatt gccataactt gtgctgtaca accattggca tcagtaacag ttacagaata
    13801 acttcctgct gtcaggtcat taatattttg tgttgttgcg ccattgctcc atgaatagga
    13861 gtaaggtgct gttccaccac taactgctaa gttgatgttt ccattatttt gtccatagca
    13921 ggaaacattc tggctggttg aaacactggc agccattgct gctgatggtt gtgtaatagc
    13981 tttagttaat gttgtcgagc aattgttgac atctgttaca gtaacagtat atgtgcctga
    14041 agttattccc gaaatgtttt gtgtagtagc accattgctc cagttgtatg aatagggcgt
    14101 agtgccaccg gaaacactta gttgaattgc agcattgtta ccaccaaaac aatttacgtt
    14161 ttgtgatgat gaaacagatg catttaatgc ctgtgccggt tgcgaaactg ttgtggtttc
    14221 acttgttgta caaccgttgt tgtcggtaac agttactgta tatgcgccag aacttaaacc
    14281 tgtgatattt tgtgaagttg caccattgct ccatgcataa gtgtaaggtg ctgtgccacc
    14341 atttacatta atagaaacag agccattaga taatccataa caagaaacat tcgttgatgc
    14401 agatattgtt gcatccaact gagccgatgg ttgagtaata gttttgtcgg cagtagttgt
    14461 acatccgttt gcatctgtga tggtaacagt gtagcttcct gcagcaagcc ctgaaataga
    14521 tgcagttgtt gcaccattgt tccataaata agtgtatgct gatgttccac ctgaagcagt
    14581 aacactcatg ctgccattcg gtacattgta acatgaaata ttttgtgaag ctgttacaga
    14641 agcactcaat gcctgagcag gttgggtaat tgtttgaatt gcatttgttg tacacccatt
    14701 gttgtctgtt atggtaactg agtatgatcc tgctgcaagc ccactgatgt tttgtgaagt
    14761 tgctccattg ctccagttgt atgagtatgg agtagtgcct ccgccaactg tgatagaaat
    14821 tgatgcattg ttgccaccaa aacagttaac atgctgcgtt gctgctatac tggcatttaa
    14881 tgcctgtgcc ggctggccaa ttacttttgt taatgttgtt gtacaattat tggcatcagt
    14941 tactgtaact gtgtaagttc ctgatgtcag attttgaata ttttgtgttg tagcaccatt
    15001 gctccagtta tatgaataag gtgaagtacc tccattaacg tttatattta tttgtccgtt
    15061 agatccacca ttacaactta cttcttctga tgattctaaa gatgcagcta atgcagctga
    15121 cggttgattg atagttttat ttaaagtagt agtacaacca ttagcgtcag ttactgtaac
    15181 ggtatagcta cctgcaatta atccggtaag attttgtgtt gtggcacctg tactccatac
    15241 aaaagtaaat ggcgaagtac caccggtaac acctacattt atgctgccgt tgttcaagcc
    15301 atagcatgaa acttctgttg aagaagatgt tgatgccgtt aaagctcctg aaggttgtga
    15361 aatattttta ttgatcgtgt agatacatcc attatcatct gtaacagtta cggtatagtt
    15421 gcctgctgta atgttactga tattttgtgt tgttgcgcca ttactccagt taaatgagta
    15481 gggtgaagta ccacctgata cagataggtt gatgtgcgca ttgtttcctc cgaaacaaag
    15541 tacgttttga gaggaagaaa ttgatgctga taaggcattg gcaggttgag aaacagttcc
    15601 ggtagcttgt aattcacaac ctttagaatc agtgatggtt acattatata taccggagct
    15661 caatccattt atattttgtg tagtagcacc attgctccat aaataggtgt aaggtgcagt
    15721 tccaccggtc acagtaagat ttagtgcacc attgccacca ccgttacaag aaacattctg
    15781 catagattga atgttggcgc tgatggttgc agaaggttgt gttaacgtaa tattgttggt
    15841 tgtgcttgta catcctttag catccgttat tgttactgag tatgtgccgg aagttaagtt
    15901 ggctaaattc tgtgttgtcg cgccattgct ccagttaaac gagtagggtg aagtaccacc
    15961 tgatacagca atgttgatgg aacctgttcc gtttccgtaa cagttgatgt tttgtgtaga
    16021 tgcgacagag ctgtttaatg ctgcagatgg ttgcgtaata ctgattgcat tttgtgatga
    16081 agtacatcca ttagcatcag taacagttac actataattt cctgcaacaa tgtttgaaat
    16141 attttgtgtt gttgcaccat tgctccatgt ataagcataa ggtcctgtgc caccattaac
    16201 ggttagcgaa acagcaccgg ttgcattgcc aaagcaatta acattagtgc ctgtggcaga
    16261 tgtggttaat gctgcagaag gttgtgctac gctaattgaa ttttgcgatg aagtacatcc
    16321 attggcatct gtaatagtaa cactgtaaac accggaagtt atattgctta agttttgtga
    16381 tgtagcacct gtattccagg aataactgta tggcgatgtt ccaccattaa cggttacatt
    16441 tatagaacct gttgcattgc cgaaacaatc tactgcaaca gtatttgctg atgacgttaa
    16501 tgaagcagaa ggttgtgtaa cagcaataga agattgtgtt gaagtacatc ctttagaatc
    16561 tgttacagta actgtatagt ttcctgctgc aatgttgctg atgttttgtg atgtggcacc
    16621 gttgctccaa gaataggagt atggtgcagt gccacctgaa acactcagat tgatttctcc
    16681 tgtagcattt ccaaaacagt ttacattcac tgcagttggt gttgcagcta aagctgcagc
    16741 cggttgactg atgtttattg aagtttgtga ggttgtacat gagttggcat cggtaacagt
    16801 aactgtatag tttcctgctg ttaaaccggt aactgatgaa ctggttgctc ccgtattcca
    16861 taagtatgta tatggtgttg tgccacctga agctgttact gatattgatc cgttatttcc
    16921 accaaaacaa tttacattaa cctttgaatt ggctaatgat aatgcagcag caggttgtgt
    16981 tatgctaatt gaactttgag tgcttgtaca tccttttgaa tctgtaactg taacggtata
    17041 gttaccggcc gtcagtccgg ttacgttttg tgtagaagca ccattgctcc atgtaaaatt
    17101 atatggtgat gtaccgccat tcactgtcag gttgattgcg cctgttgcac caccataaca
    17161 tccaacacct gttgcacttg cagtggctga taatgccgct gcaggttgtg ttaatgttgc
    17221 agttgcagtt gtgctgcaat tttgcgagct ggtaacagtt actgtataag tgcctgctcc
    17281 ggcattgctt aagttctgtg aagatgcacc attgctccag gagtaagtgt atggtgctac
    17341 actgttagaa acagttgtag taattccacc tgtctgctga ccaaaacaat taatatttgt
    17401 tcctgtcaga cttacctgtg gagttttgcg aagatttatt ataatggtgt ccttaactgt
    17461 tgcgcaaaca tttttaggac ccataatcag cattaccgaa cctgcattaa tatcagcagc
    17521 tgatggaata taatatccat ttttaactgt gtcgcttgga ctaaactgac ctgttcccga
    17581 tgttgtccat tgaacagcac cggtgaaact tgattgtgag taggcaatgg ttccattgaa
    17641 tgctattgaa tcctgaccac caccttttgt acagatattt ttatctgttc cggcactgtt
    17701 tactaaatag tccggtaagt tgataatagt ttttgagcta agtttatgtg caaaccacca
    17761 tgtgttaatg gtgttacttt caccaatagt attttggtcg gagttggcag ggcttgatgt
    17821 ccatgtatgg caaggtgatg ggcaaccgcc aaggtttgtt aagccaccaa ctaaaacgtc
    17881 atcataaaat aatgaatcta caaactgtgc actgccggga cggataactg acattgagaa
    17941 tcctgtttca tgattctcta catcatacat tggcaggttc accaatccgg attcaatact
    18001 taatattact gatacaggat tacccacagg aattaaattt cctaaaccat cttttccatc
    18061 ccaatacaaa caacttgaac cggacggcac ttgtggaaag aataatttcc ggtctgctgt
    18121 accggcatca aatttattgt tgttatttct gtcaatcaca actgtaacgt cagatgtttt
    18181 tattaaacca acgttgatgc aaatagaatc cctgacacaa cgctccagtg taggaggccc
    18241 tgttaagaac gacattgaac cattgggata cgctataata tccgggtctg taagaaataa
    18301 acgatattct acaggaacag caaatccggt tttagatctt ctgtcaattg ttgcatttcc
    18361 tgaattggat gtaccaaaac ggttacatga tacacgaaat ttaaatggct taataccatt
    18421 cagatcaact ttagttacgg aactgtcctg tccataaata aagaaagatc cgaaaaatgg
    18481 atttgaatat gattcagtat taaaacccca gttttttgaa aatagacgtc ctgtgacttt
    18541 tgtgttattg gaagtgttta caactgttac atcaaatata cctaagccta attctgtgtt
    18601 acctgcaact gatgtcccac tacctttatt gaattcaata taataagcac ccggaagacc
    18661 tgcagtagga ttacaaacat aaccgttata acctgctgcg ttaaccacat tcggacctgc
    18721 aatggcttgc gaatgatagg tgataaatcc ttgtgcatta ccggttggta atttttgtgg
    18781 gccaaaaatg atattcccat taggatcttt aatacgaaca taccagtctg agttggtaga
    18841 acgctgacca attccaaaat agattttttc ctgtgcagga ttattgattc gcacatatag
    18901 cctgtcttcg tcagtggcag agtagcccgc aaaagtacca taggtagttt gcgtatttag
    18961 aatcataaga ttcgttgcct ttgttgtatc aggcttgaac tgtggcgatc cgtcagcata
    19021 tgttttatca tggctgacaa ttagaaacaa agtaaacaac agcgatgctg tcatcaactt
    19081 tagttgaaat ttctgaatca ttttgtttat aaaagttaac atctttccca taagttggtg
    19141 cgattttaat acactgatat gggcattcaa cgttgatgtg caatgtaagg ttacagggta
    19201 tttagttttg taatgtcatt taaaaattat tattgaattt gttgtaaccc tgtttgaaaa
    19261 gtaccgtatt aaccggaaaa acattttcaa gtttaaattt tccagaaatg aaaaaagtaa
    19321 tatttagttt gttttttgtc atagctgcat tagcagtaaa agcacaaaca atcattggta
    19381 aagttgatgt ggatggcgtt ccacagttta cggcagcaat cgctgattgc aaaagcgaac
    19441 ttcaaaaagt aacaagcagc aaaggcacgt tttctaccat gaaactgcag gatattactt
    19501 tctcccaaat gtctaaaggg ca
//
LOCUS       DAOQNP010000062        18979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1021_length_18979_cov_9.450962, whole genome shotgun sequence.
ACCESSION   DAOQNP010000062 DAOQNP010000000
VERSION     DAOQNP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18979
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1021_length_18979_cov_9.450962"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PKY09_09040"
     CDS             <1..1451
                     /locus_tag="PKY09_09040"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNB13043.1"
                     /translation="GECYVTNDGAETDLDLGHYERFLNVPTSQANNVTTGRIYQTVIN
                     KEREGAFLGKTVQVIPHITDEIKRRIKILGETGEYDIVITEIGGTVGDIESLPFVEAI
                     RQMIWEMGSNCCVIHLTLLPYLNVTGELKTKPTQHSVKTMLEYGVQPDILVCRSEKSI
                     GADLRRKIALFCNVNVNAVIEAVDASSIYEVPLHMLKEQLDKVVLNKLKLSFRQEPDL
                     TVWKAFLGKLKHPVSEVKIGLIGKYIELKDAYKSIAEAFIHAGVANECKLNIEWIHSE
                     KIEEQNVADKLKGLSGILVAPGFGDRGIEGKIAAIKYARENKIPFLGICLGMQCAVVE
                     FARNVLGLKDAHSSEMNKETKNPVIDLMAKQKEISAKGGTMRLGEYPCSLAKSSKVAQ
                     AYGKTKIYERHRHRYEFNSKYQKRFEDAGMIATGINPADNLVEIVEIKNHPWFVGVQF
                     HPEYKSTVENPHPLFVRFVKAASEFAKGAIQI"
     gene            complement(1448..2380)
                     /locus_tag="PKY09_09045"
     CDS             complement(1448..2380)
                     /locus_tag="PKY09_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13044.1"
                     /translation="MHHRFSVFFLMLFSCLATISCKKEGNKYDTPVKDIEQFFSETGA
                     PVQQFSFSSAANINQTTAMGNTFIISANSFTKKDGSAVTGNIDIKLRECVSKSDLILS
                     NLTTMSGSKFLSTGGIFYLAAYQNDVALSLSNGKTINFFLNSTFGNGQMLHRYYSSSL
                     PLENNSSDVSWTADADTQMINLLSSSNQSHYAYVMKNTGWQNAAEPVFSNSSRKKITI
                     DTGTDYETSNTAVFVSVNGSNTICKAKSVTLGVWEVENFPVNLNVTVLALSKVNDSYF
                     FSKSSVIVSTDISVPLVMNSITLPALKTELEQLP"
     gene            2754..3524
                     /gene="surE"
                     /locus_tag="PKY09_09050"
     CDS             2754..3524
                     /gene="surE"
                     /locus_tag="PKY09_09050"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNB13045.1"
                     /translation="MSKPTILVTNDDGITANGIHALVEAMREIGHVIVVAPDSPQSGM
                     GHAITIHNPLRLHKVDVFKGIDAWQCSGTPVDCVKLAVNKILNRKPDICVSGINHGSN
                     SSINVIYSGTMSAAMEGAIEGIPSIGFSLLNYKFDADFSAARHFAGVVTRAVLQGGPW
                     RELLLNVNIPYLPLNEMKGIKICRQAMAKWKEEFDERLDPAQQKYYWLTGKFINPDLG
                     EDTDEWALANGYVSVVPVEFDFTAHHAIPVLNKLQWNA"
     gene            3517..3801
                     /locus_tag="PKY09_09055"
     CDS             3517..3801
                     /locus_tag="PKY09_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13046.1"
                     /translation="MLKKIPDTFHIGAISALVLSVLAWLLISRTESFIVASALKDYLA
                     FPRLYFIVFALQLVFLRIMMVNLQKQHFARGWMFSVFIFALFVFYLIKKS"
     gene            3804..4091
                     /locus_tag="PKY09_09060"
     CDS             3804..4091
                     /locus_tag="PKY09_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13047.1"
                     /translation="MFSKDKFITGFISGLIAPVAGFYLYYIALFRQMDFTGFYNTLVS
                     NNTLAAAISLSLLANLALFFVFDKFGKLNGSRGVIAATFIYGFYIVYLKFI"
     gene            4097..5212
                     /gene="lpxB"
                     /locus_tag="PKY09_09065"
     CDS             4097..5212
                     /gene="lpxB"
                     /locus_tag="PKY09_09065"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011710814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNB13048.1"
                     /translation="MRYYIIAGEASGDLHASNLVLQLRKFDTEAVMRGWGGDKMQEAG
                     VEIVKHYRDLAFMGFVEVFKNLRTILRNLENCKEDILAFNPDVVILVDYPGFNLRIAE
                     FLKKKNIKVFYYISPQVWAWKQSRVHKIKRDVDKMFVILPFEKDFYKKFGFDVVFAGH
                     PLLDEIVKIKNQPAKALSQLNKDKKIIALLPGSRMQEIKEMLPVMLKAVSTFKNFNPV
                     IAAAPNIPDAFYQSMADKDITIVHGRTYDILQHAYAALVTSGTATLETALFKVPEIVC
                     YKGNRFSYLIARQLVKVKYISLVNLILDRMLVKELIQDDMNEKTLTTEMRLLLEDEKY
                     RSNILAGYDELEAMLGMAGASEKTARKMVDLLKGTSV"
     gene            5247..5783
                     /locus_tag="PKY09_09070"
     CDS             5247..5783
                     /locus_tag="PKY09_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13049.1"
                     /translation="MKTTSYVLVLIATIMLSACQKQEAETDNEPQVAATCDDGIMNQG
                     ETDIDCGGPCPLCQATMTAMVDADTFKAVFNTITSKYSNGAFFMSGSSDLGSLTIIYS
                     GPLNIGAVSNAQATYVKSSTSVTYIASNANLTFNAINFTDSIAEGTFSFNAINFNSPT
                     DTVKVTNGFFKRISVKNN"
     gene            5908..7245
                     /locus_tag="PKY09_09075"
     CDS             5908..7245
                     /locus_tag="PKY09_09075"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNB13050.1"
                     /translation="MKNLIIILIAVMLTNIVNAQDLKAFKSDKELWGYKDASGKEIIP
                     AKYNYAWGFNEGIARVRNKKMKYGFIDITGKEVIPAKYDETKDFYEGLAAVNLDNKWG
                     FIDRTGKIIIPLKFDEVGYFSEGLAKVRVGAVRGGKYNTLSGGKYGFIDQSGSEIIKF
                     KYDNAGDFSEGFAYVKVGKKYHFIDKKENELGLLEYDDAGDFSEGLAKVRIGNYFDGK
                     WGYIDTTGKRVIEVKFEEASDFYNEEARVKLNGVSMYIDKTGSNDEERKKIAEEEAEK
                     KKQERKALIDRAIENAKRDTMAKEVFALYQNENQKVVDKVKELQRYVESTMIYSEKYG
                     FNKSNWVPMSIEINKQTKVTQAQVNSFKDLLKKYELTKGKNELYDRISVNLSKTTDFI
                     AACSIWGDYIYTLGANDDGKKELERVDDSLIDLKKHDDALRSFITLYKKRNSL"
     gene            7385..8188
                     /locus_tag="PKY09_09080"
     CDS             7385..8188
                     /locus_tag="PKY09_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009412261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HNB13051.1"
                     /translation="MNRRYLLLTVFLFSCASQKNLNTGTVQQNSLTVGGRLFTSVFQQ
                     QAAEYKALCFQAYNIAALRLDQAIGSTVSSKPKAVITDIDETVLDNSPYSVHRALEGK
                     DYESISWVDWTARAKADTLAGALTFFKYAASKNVEVFYLSNRAEKERVGTLENLKKFG
                     FPNADDAHLILKTDVSSKEGRRQKITETYDVLLLIGDNLADFSSLFDKKTTAERTQNV
                     QSIAYEFGKKFIVLPNANYGDWESALYFYDYKRTPAQRDSILKSVLKNY"
     gene            8319..8762
                     /locus_tag="PKY09_09085"
     CDS             8319..8762
                     /locus_tag="PKY09_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNB13052.1"
                     /translation="MASINPHINFNGNAEEAFNFYKSVFGGDFVKVVRFKDLASPEMP
                     IDKKEENKIMHIALPIGKHSLLMANDVPEFLGRTNENENRSKIVISAETKEEADKLFN
                     GLSSGGTVEMPIADSPWGSYFGMFRDKYGIEWMVDFDQRYNGQVR"
     gene            complement(8817..8890)
                     /locus_tag="PKY09_09090"
     tRNA            complement(8817..8890)
                     /locus_tag="PKY09_09090"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8854..8856),aa:Met,seq:cat)
     gene            complement(8955..10190)
                     /locus_tag="PKY09_09095"
     CDS             complement(8955..10190)
                     /locus_tag="PKY09_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNB13053.1"
                     /translation="MLLLYSIGIRLYHLAAALASPFNDKAKAFTEGRRNIFQKVNQEL
                     KNETAKRIWFHCSSYGEYEQGKPVLQQIKKLFPQYKIVLTFFSPSGYKQGIKENLADF
                     IFYLPCDSSTNAHKWLDYINPHMAIFVKYDFWFHYINGLSKRHIPFIYISAIFRTDQF
                     FFKWFGGAFLKELKKASRFFVQNDTSLNILNNKGITQVEVSGDTRFDRVIQLPELEFT
                     DEKIESFSVGERIIVAGSTWPEDHKLLAGYCNSEEIKLLLVPHEIHESSYKDFTYACR
                     KNVVRHSKATKEELKKADVLIIDKIGMLSKIYRFATITYIGGGFGKGIHNTLEAAVYG
                     KPVIFGPNFQKFAEAKLLIESGGAFTINNLTEMKTVFDDLLNNNKKREQAGAICENVV
                     KSNSGATMIITNWLQNHLH"
     gene            complement(10193..10948)
                     /locus_tag="PKY09_09100"
     CDS             complement(10193..10948)
                     /locus_tag="PKY09_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13054.1"
                     /translation="MQVKVFLLAILISFSSLQTAAQALVRDSAIVIPFFGIGGEFHLP
                     GGDMKERFGADYGVQGVFGVKTPKNFLYSIQGGFFFGNKINQPGLLAGIASSYGTIIG
                     SDGRTADVRLFERGYHISAQAGKVFAFKKPNPNSGIWISAGAGFMQHKIRIETIGNTV
                     KGLSKKYKKGYDRLTNGIIVEEFIGYVYYGNKKLINFYAGFNFIQGFTQGRRDYQFDT
                     MKADDQSRVDLLYGFKAGWILPFYTNPDKFYYY"
     gene            complement(10950..11396)
                     /locus_tag="PKY09_09105"
     CDS             complement(10950..11396)
                     /locus_tag="PKY09_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13055.1"
                     /translation="MNKIFVIASIMLMLSVSSYHYKTGWTNEVFADTTDINPNKSSEL
                     SLLMRQMYNHAAEARKAVSEQRTVSYPKTFLNINTAKPTDDKTKNEYYTTFADLYLQT
                     LDSYENATNTNRVKSFNNVVNACLACHSSHCPGPVPKIKRLLIPLD"
     gene            complement(11389..11847)
                     /locus_tag="PKY09_09110"
     CDS             complement(11389..11847)
                     /locus_tag="PKY09_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNB13056.1"
                     /translation="MRKLILVAVATVMSLSLMAQDKKASHFGKKITEENAQPASVVDR
                     MLTTKDTVNVKIQGKIASVCQKKGCWMMMETSDGHTIRVRFKDYEFFVPKDCAGKTAI
                     IEGIAFNDVTSVAELRHYAEDANKSKEEIEKITQPEKKPAFEAEGVIIYE"
     gene            12049..14982
                     /locus_tag="PKY09_09115"
     CDS             12049..14982
                     /locus_tag="PKY09_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNB13057.1"
                     /translation="MFLKRCFWCLLMVLLPFVSYSQKKLPGFINPVSYHWADSVLDEM
                     TVDEKIGQLFMVQMQSVWPEKDLDSLESLIRNYHLGGLIVFKGGPYRQALCINRMQQA
                     AKIPLLVSIDGEWGLSMRMDSTIRFPRQMTLSAIHDDSLLSDAFYEIASQCKRMGIQL
                     NFAPDIDINNNPQNPVINTRSFSDDKFEVAEGGILYMQALQNQHVLACGKHFPGHGDT
                     DTDSHYALPVINKSANQLDTLEWFPFRQLIKNGVSSIMTGHLNVPLLDDENYPASLSK
                     RIINGYLKDDLDFKGLVITDALNMKGAILADAKPGDIELQALLAGNDILLMSENVKPA
                     IEKIKTALQKRKLRKSALDEKVRKILMAKHWAGLTNYAPIDTANLYYDLNNADASYLN
                     YRLYEKSLTLLRNNAVLPLLNSSAERMASLVINDTLENTFQQTLSLFAPVQNFVMSKD
                     ASPATVDSIAAVLSGFTKVIISIHNTTTKPQFNFGITESMNSVIQKVSMRTKAVVVLF
                     GNSYCLNKLVIPDQTKALIIAYEDTYLPQHITAQALFGAVPFNGRLPVSPGLSFHRGD
                     GLNLQATGVIKYTMPAELNIDLTCLQKVDSIINDAIKQKAFPGCQVYASVDGKVIYNK
                     SFGYTTYADTQAVENDMIYDIASMTKIDATALAAMWLYDHHLLDLKQTVAYYLPDYEK
                     TNKGSLTVEQLMTHTAGLQAWIPFYKEGLTKNGTMMKKYFSSSKHDDYTVQITDSLFM
                     NPKFYSKIDKQIKESPLTEKGKYVYSDLGMLLLQKIIERQAGKKMDVLLNEEFYQPLG
                     LSHLLFNPRSKLALKNIVPTEYDTVFRQTLVHGYVHDPAAALFGGVAGNAGLFGNAQS
                     VGVLMQMLLNEGSYAGKQYISKATVDTFTEAPIPGIRRGLVFDKPETNPAKGSPTCKS
                     ASPQTFGHQGFTGTCTWADPSNQLVYVFLSNRIFPSAANQKISKLSVRTEIQQVFYDC
                     LRKVR"
     gene            15047..16186
                     /gene="bshA"
                     /locus_tag="PKY09_09120"
     CDS             15047..16186
                     /gene="bshA"
                     /locus_tag="PKY09_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNB13058.1"
                     /translation="MKIGIVCYPTFGGSGVVATELGKALAAKGHRIHFITYSQPVRLD
                     SFSENISYHEVSVSNYPLFEYAPYESALTSMLVDVAMHEGLDVLHVHYAIPHASVAYL
                     AKQILISKGIYIPVITTLHGTDITLVGRDNSYEPVVTFAINQSDGVTAVSESLKRDTY
                     ANFEITKHIEVIHNFIDLKRFSKKPKDHFKRAIAPGGERLLVHTSNFRKVKRVNDVIQ
                     VFARIHKRIPSRLLMIGDGDERAGAEKLCRELQLCDDVRFLGKQDAIEEILSVCDLFI
                     MPSETESFGLAALEAMACQVPVISSNTGGMPELNLDGVTGFTSNVGDIEDMAEKAYHI
                     LKDDQTLNVFKQNALKRASDFDINVILPRYENYYQSVIQNFTVAS"
     gene            16495..16944
                     /gene="rplM"
                     /locus_tag="PKY09_09125"
     CDS             16495..16944
                     /gene="rplM"
                     /locus_tag="PKY09_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNB13059.1"
                     /translation="MDTLSYKTISLNAKTAKKEWVLIDATDMVLGRLSSEVAKILRGK
                     HRPGFTPHVDTGDNVIIINAEKVKLTGKKMTQKVYVRHTGYPGGQRFTSPEKLMDTYP
                     ERILENAIHKMLPKNRLGNALKKNLRVFAGSEHSHQAQNPRKITLDI"
     gene            16951..17337
                     /gene="rpsI"
                     /locus_tag="PKY09_09130"
     CDS             16951..17337
                     /gene="rpsI"
                     /locus_tag="PKY09_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNB13060.1"
                     /translation="MNNIHKIGRRKTAVARVYLTPGTGTIVINNREFKNYFTTGPLQN
                     KVTQAFAVINANEQFDVKANVMGGGITGQAEAIRLAISRALCEMNPENRPALKKEGLL
                     TRNPKMVERKKSGQKKARKKFQFSKR"
     gene            17357..18226
                     /gene="rpsB"
                     /locus_tag="PKY09_09135"
     CDS             17357..18226
                     /gene="rpsB"
                     /locus_tag="PKY09_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNB13061.1"
                     /translation="MSINTQELLDAGCHFGHLKRKWNPKMAPYIFMERNGIHIIDLNK
                     TAAKLEEATAAIKQIAKSGKKILFVATKKQAKEIVANAAKSVGMPYITERWPGGMLTN
                     FATQRKSVKKMTGFDRFMKDESFNNISKKERLQVSRQKAKMEKLIGSIADLTRLPAAL
                     FIVDVLKEHIAVSEAQKLGIPTIAICDTNTDPTLIDFPVPANDDATKSISLIMNAMIK
                     AIEEGLEERKMDKEADREREAAAEEESGERGDGEKEFVMSEEGDDSGEGKSKAPAKKA
                     TTRSRKPTTRKKD"
     gene            18254..>18979
                     /gene="tsf"
                     /locus_tag="PKY09_09140"
     CDS             18254..>18979
                     /gene="tsf"
                     /locus_tag="PKY09_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNB13062.1"
                     /translation="MSTVTISASDVNKLRQMTGAGMMDCKKALMEANGDFEKAIDELR
                     KKGQKVAANRADREAKEGYIVAKTNNDATKGYLVALSCETDFVAKNAEFVKFAEDVLE
                     TALTNNAANLDALLALQLQGTTISEKLIEMVGKIGEKIEITRYVVIEAPKVVVYIHPG
                     NRVVSMVGLSSATAAHEAGKDVAMQIAAMNPVAIDKDDVDQSTLDREIEIGKEQARAE
                     GKPENMIEKIAMGKLNKFYQESTL"
BASE COUNT         6189 a         3227 c         3797 g         5766 t
ORIGIN      
        1 atggagaatg ttatgttacc aatgatggtg cagaaacaga tctcgacctt ggacattatg
       61 aacgtttttt aaatgttccc acatcacagg caaataacgt taccaccggt cgcatttatc
      121 aaacggttat caataaagaa cgtgaaggag cttttttggg taaaacggtt caggtaattc
      181 cacatattac cgatgaaatt aaaagacgta taaaaatact cggtgaaaca ggtgagtatg
      241 atattgtaat tactgaaatt ggtggaactg ttggtgatat tgagtcattg ccttttgtgg
      301 aagctattcg tcaaatgatt tgggagatgg gcagcaattg ttgtgttatt catcttacac
      361 ttttgcctta cttaaatgtt acaggcgaat taaagactaa gcccacacag cactcggtaa
      421 aaaccatgct tgaatatggt gtacaacccg atattttggt ttgccgcagc gaaaaatcca
      481 ttggagccga tttgcgcaga aaaattgctt tgttctgtaa tgtaaatgtc aatgcagtta
      541 ttgaagctgt tgacgcaagc agcatttacg aagttccgct gcacatgctc aaagagcagt
      601 tggataaagt tgtgctcaat aaactaaaac tttccttcag gcaagaacct gacctcacag
      661 tatggaaagc ttttttagga aaattaaaac atcctgtttc tgaagtaaaa atcgggttga
      721 taggaaaata tattgagctg aaagatgcct ataaatctat tgccgaagca tttattcatg
      781 caggtgttgc taatgagtgc aagttgaata ttgagtggat acattcagaa aaaattgaag
      841 aacaaaatgt tgccgataag ctaaaaggat tgtcaggaat attggtggct cccggttttg
      901 gcgacagagg tattgaagga aaaattgcag ctataaagta tgcaagagaa aataaaattc
      961 cttttcttgg aatttgtttg ggtatgcaat gtgcagtagt agagtttgcc cgcaatgtgc
     1021 tcggactgaa agatgcacac agttcagaaa tgaataagga gacaaaaaat cctgttattg
     1081 atttgatggc aaaacaaaag gaaatttctg caaaaggtgg taccatgcgc cttggagaat
     1141 atccatgttc tttggcaaaa agcagtaaag tggcgcaagc ctatggcaaa actaaaatct
     1201 atgaacgcca tcgtcaccgt tatgagttta acagcaagta tcagaaaaga tttgaagatg
     1261 caggaatgat tgctacagga ataaaccctg ctgacaacct ggtagagatt gttgaaataa
     1321 aaaatcatcc ttggtttgta ggtgttcagt ttcatcccga atataagagt acggtagaaa
     1381 atccacatcc gctatttgta agatttgtaa aggcagccag cgaatttgcc aaaggcgcca
     1441 ttcagattta aggcagttgc tctaattctg tttttaatgc cggtaaggta atggaattca
     1501 ttactaacgg tacggatata tcagtgctaa cgatgacaga tgatttagaa aagaaataac
     1561 tatcgttgac cttcgacaat gccagaaccg ttacattgag attgacagga aagttttcta
     1621 cttcccacac tcctaaagta acagattttg ctttgcaaat ggtgttgctg ccattaacag
     1681 aaacaaaaac cgcagtattg gaagtttcat agtctgttcc tgtatcaata gtaatttttt
     1741 ttctgctact attactaaaa accggttctg ctgcattttg ccagcctgta tttttcatca
     1801 cataggcata gtgcgattga ttggaagagg ataacagatt aatcatttgt gtatcggcat
     1861 cagcagtcca tgaaacatcg ctactattat tttcaagagg taatgaactg ctgtaataac
     1921 gatgcagcat ctgtccgttg ccaaaagtag aattgagaaa aaagttgatg gttttgccat
     1981 tagataaact taatgcaaca tcattctgat aagccgcaag ataaaaaatg cctcctgttg
     2041 ataagaattt actgccggac atggtagtga ggttgctgag gataagatcg cttttgctca
     2101 cacattcacg cagtttgatg tcaatattac ctgtaactgc actgccatcc tttttagtaa
     2161 aactgttggc tgaaataata aacgtattgc ccattgccgt agtttgattg atgttggcag
     2221 cagatgaaaa agaaaattgt tgcacaggag cccctgtttc actgaaaaat tgctcaatat
     2281 cttttacagg agtatcgtat ttatttcctt cttttttgca ggatatagta gcaagacagg
     2341 aaaatagcat aagaaagaaa acagaaaaac gatgatgcat tatttagaac tttatatgca
     2401 ataatataaa ggtaaacagt ataagtcaat agacttgcaa cagagttata attgattttt
     2461 acacagcata aaaaataaga atgtttaaaa atcaatagag taagacattg agagtcatca
     2521 ctggtaggaa ataacaccgt ttttttgccg aagcagaaaa agcatgcttc cgcttattta
     2581 ggttttactt caaaaatact ttgttttttt aaaccaagtt atgttcatta tcaaacaact
     2641 gaatagtgta atgtacccca ttctttaaag tgggttttgg ggagcattaa actccaaact
     2701 tttgcattaa aataagaaat atcacaaagc gtaagaactg ctatcttcgc agcatgtcaa
     2761 aaccaacaat tttagttacc aatgatgatg gcataacagc caatggcata catgccttag
     2821 tggaggccat gcgcgaaata ggccatgtta ttgttgttgc accggacagc ccacagtcag
     2881 gcatgggtca tgccatcact attcataatc cgcttcgtct gcataaagta gatgtgttta
     2941 aaggtattga tgcatggcaa tgcagtggta cacctgttga ttgtgtaaaa ctggctgtca
     3001 ataaaatttt aaatcgcaag cccgatatct gtgtaagcgg aatcaatcac ggtagtaact
     3061 catccatcaa tgttatttat tcaggaacca tgtctgctgc tatggagggt gccatagagg
     3121 gtattccaag cattggtttt tccttgttaa actataagtt tgatgctgac ttttcagcag
     3181 cacgtcattt tgcaggtgtt gtcacccgtg ctgtgttgca aggcggaccc tggcgcgaac
     3241 ttttacttaa tgtaaatatt ccttacctgc cgcttaatga aatgaaggga ataaaaatat
     3301 gccgtcaggc aatggccaag tggaaagagg agtttgatga aagattagac cctgcgcaac
     3361 aaaaatatta ttggcttacc ggaaagttta ttaatcctga tttgggagaa gatactgatg
     3421 aatgggcttt ggccaatggc tatgtttctg tagttcctgt tgagtttgat tttacggcac
     3481 atcatgccat ccccgtatta aacaaattgc agtggaatgc ttaaaaaaat ccctgacaca
     3541 ttccatattg gcgccatatc agcattggtt ttgtcagtcc ttgcatggct gcttatcagc
     3601 cgtaccgaat catttattgt agcatctgca ttaaaagatt atctggcatt tccacgattg
     3661 tattttattg tgtttgcttt gcagcttgtt tttttacgca taatgatggt caatttgcaa
     3721 aaacaacatt ttgcccgtgg atggatgttt agtgttttta tttttgcact gtttgtattt
     3781 tatctgataa aaaaatcata atcatgttca gtaaagataa gtttataaca ggattcatta
     3841 gtggcctcat tgcaccggtt gccggttttt atctctatta tattgcacta ttccgtcaga
     3901 tggattttac cggattttac aacaccttgg tttcaaacaa cacgcttgct gcagctatca
     3961 gtttgagttt gcttgctaac ctggcactgt tctttgtgtt cgataaattt ggtaagctaa
     4021 atggttcgcg cggtgtcatt gctgcaacat tcatttatgg attttatatt gtttatctta
     4081 aatttatcta atcctgatgc gctactatat cattgccggt gaagcctcag gcgatttaca
     4141 tgcatccaat ctggtattgc aactgcgtaa attcgataca gaggctgtaa tgcgcggatg
     4201 gggaggcgat aagatgcagg aagcaggagt ggaaatcgta aaacactatc gcgatctggc
     4261 atttatggga tttgtagaag tgtttaaaaa cctgcgtaca attttgcgca atcttgaaaa
     4321 ttgtaaagaa gatattcttg catttaatcc tgatgtagtt attttagttg actatccggg
     4381 cttcaacctg cgcattgcag agtttctgaa gaagaaaaat attaaggtgt tttactatat
     4441 ctcacctcag gtttgggcat ggaagcagtc gcgtgtacat aaaataaagc gtgatgtgga
     4501 taagatgttt gtgatattac ctttcgaaaa agatttttat aaaaagttcg gttttgatgt
     4561 tgtatttgcc ggacatccgc tattagacga aattgtaaaa atcaaaaatc aaccggccaa
     4621 ggcattgtcg cagttaaata aagataaaaa aattattgca ctgttgcctg gcagtcgtat
     4681 gcaggaaata aaagaaatgc ttcctgtgat gctgaaagct gtctctacct ttaaaaattt
     4741 taatcctgta attgcagcag cacccaacat tcctgatgcc ttttatcagt caatggccga
     4801 taaagacatt accattgtac atggacgcac ctatgatatt ttgcagcatg cttatgctgc
     4861 tttggtaaca agtggtacag caacattaga aacagcattg tttaaagtac ctgaaattgt
     4921 atgctataaa ggaaaccgtt tttcatacct tatagcgcga caattggtta aggtgaaata
     4981 tatttcttta gtaaacctta ttcttgaccg catgttggta aaggagctca ttcaggacga
     5041 catgaatgaa aaaacgctga caacagaaat gagattgttg cttgaagacg aaaagtacag
     5101 aagcaacatt cttgcaggtt atgatgagtt ggaagctatg ttaggcatgg ctggcgcttc
     5161 tgaaaaaaca gcacgaaaaa tggttgattt gctgaagggg actagtgttt aataaaattt
     5221 atttaccttt atgctaaatt ttttttgtga aaacaacaag ctatgtttta gttcttatcg
     5281 ccaccataat gctttctgca tgtcagaaac aggaggcaga aacagataac gagccacaag
     5341 ttgccgctac atgtgacgat ggcatcatga atcagggtga aacagatatt gactgtggag
     5401 gaccctgccc cttatgtcag gcaacaatga cagcaatggt tgatgccgat acttttaaag
     5461 ctgtatttaa taccatcacc agtaaatatt ctaatggtgc attttttatg tcagggtctt
     5521 ctgatttggg atcgctgaca attatttact caggtccatt gaacatagga gctgtatcta
     5581 atgcacaagc tacgtatgtg aagagttcaa cctcagtaac atatattgca agcaatgcca
     5641 atttaacttt caatgccatc aattttacag acagcatagc agaaggaacg ttttcattta
     5701 atgccatcaa ttttaattct cctaccgata cagttaaagt taccaatggg ttctttaaaa
     5761 gaatcagtgt aaaaaataat tgaagcaaca aggtagttga agaagaataa cggcttagga
     5821 aattattgta ttagacttag agtaaaaatt ttagtagtaa actagataga attaacttta
     5881 ttaagtagat aataatcacc aaaaaatatg aaaaatctaa ttattatttt aattgcagtg
     5941 atgttgacaa acatcgtaaa tgctcaagat ttaaaagcat ttaaaagcga taaagagctc
     6001 tggggttata aagatgcaag cggaaaggaa atcattcctg caaagtataa ttacgcatgg
     6061 ggttttaatg aaggtattgc aagggttaga aataagaaaa tgaagtatgg atttattgat
     6121 ataacaggta aggaggtaat cccggcaaaa tatgatgaaa cgaaggattt ttatgaggga
     6181 ttggctgctg taaatttaga taataagtgg ggatttattg atagaacagg taaaataatt
     6241 attcctttaa agtttgacga ggtaggatat tttagtgagg gcttagcaaa agttagggtt
     6301 ggcgcagtaa gaggcggtaa gtataataca ttaagtggag gtaaatatgg ttttattgac
     6361 caatcaggaa gtgaaattat taaatttaaa tatgataatg caggtgattt tagtgaaggc
     6421 tttgcttatg taaaggtagg gaaaaaatat cattttattg ataagaagga gaatgaactt
     6481 ggtttgcttg aatatgatga tgcaggtgat tttagtgaag gattggcaaa ggttaggatt
     6541 ggaaactatt ttgatggtaa gtgggggtat attgatacaa caggtaaaag ggtaattgaa
     6601 gtaaaatttg aagaagcaag tgatttttat aatgaagaag caagagtaaa gttgaatggt
     6661 gtatcaatgt atattgacaa gacaggaagt aatgatgaag agagaaaaaa gattgctgaa
     6721 gaggaagccg aaaaaaagaa gcaggagaga aaggctttaa tagatagggc aatagaaaat
     6781 gcaaaaagag ataccatggc taaagaagtt tttgcactat atcaaaatga gaatcaaaaa
     6841 gtagttgata aagtaaaaga attacaacgc tacgtggaat caacaatgat atatagtgaa
     6901 aagtatggat ttaataaaag caattgggtt cctatgtcaa tagaaattaa taaacagaca
     6961 aaggttaccc aagcacaagt aaattctttt aaagatcttt tgaaaaaata tgagctaaca
     7021 aaaggtaaaa atgaattgta tgatcgtatt tctgtcaatc tttcaaaaac aactgatttt
     7081 attgcagcat gtagtatctg gggtgactat atttatactt tgggcgcgaa tgatgatggt
     7141 aaaaaggagt tagaaagagt tgatgatagt ttgatagatc tgaaaaagca tgatgatgca
     7201 ttacgttcat tcattactct ttataaaaag agaaatagtc tttaataaac taattccatt
     7261 ttacttacat aaaggtcagg ttgtttaaag ttgtaattag atgttaatgc ttagtaggaa
     7321 aaccaaagta tgaggatcca attttttgaa aaaaatacat ttatctttac cgaaatattg
     7381 ttttatgaat agaaggtatt tactccttac agtttttctt ttttcttgtg catcgcaaaa
     7441 aaacttaaac accggtacag ttcaacaaaa tagtttaaca gttggcggac ggctctttac
     7501 ttcagtattt cagcaacagg cagccgaata taaagcactt tgttttcagg cttacaacat
     7561 tgcagcttta agattggatc aggcaatcgg cagcactgtt tcttcaaaac ctaaggcagt
     7621 aattacagat attgacgaaa ctgttttaga caattctcct tattctgttc atagggcatt
     7681 ggagggaaaa gattatgaat ctatttcgtg ggttgactgg acagcacgtg ccaaagccga
     7741 cacacttgca ggtgcactga ctttttttaa atatgctgca tctaaaaatg ttgaagtgtt
     7801 ttatctttct aatcgtgctg aaaaagaacg tgtaggaacg ttggagaatc ttaaaaaatt
     7861 cggtttccct aatgctgatg atgcacattt gattttgaaa acagatgttt caagcaaaga
     7921 aggaagaaga caaaaaatca ctgaaacgta tgatgtactc ctattaattg gtgacaatct
     7981 tgctgatttc agctcattat ttgataagaa gacaacagca gaacgcacac agaacgttca
     8041 aagtattgct tatgaatttg gaaaaaaatt tattgttctg cccaatgcaa attatggcga
     8101 ttgggaaagc gcattgtatt tctatgatta caaaagaaca cctgcacaaa gagattctat
     8161 tttaaagagt gttttaaaaa actattaaaa taggtattca acataaatca atttttctaa
     8221 aacaacggtt tttacagtat attaattagt aaagtcttat atgaaaagaa aatttctgaa
     8281 agtaattaat agtaaaataa acattaaaat ataaaaaaat ggcaagtatt aacccacaca
     8341 tcaactttaa tggaaatgcc gaagaagcat ttaactttta caaatcagtt tttggcggtg
     8401 actttgtaaa agttgttcgt ttcaaagacc tcgcaagccc ggagatgccg attgacaaga
     8461 aagaagaaaa caagattatg catattgcat tgcccattgg caaacacagt ttattaatgg
     8521 caaatgatgt tcctgaattt ttaggccgaa ccaatgaaaa tgagaacagg agcaaaattg
     8581 ttatcagtgc agaaacaaaa gaagaagcag acaaattatt taacgggctt tcttccggtg
     8641 gcacagtaga gatgcctatt gctgatagcc cctggggttc ttattttggc atgttcagag
     8701 ataaatatgg tattgaatgg atggtggatt ttgatcaaag gtataacggg caggtaagat
     8761 aatttgattg gcaaaaaaat aaggctgcca caaacttttg tgacagccta ttcttgtagc
     8821 gaagggggga gtcgaacccc cgacctcagg gttatgaatc ctgcgctcta accatctgag
     8881 ctacctcgcc tgattttgag gctgcaaaag tagtattccc tttcaattgt aaaaacaatt
     8941 tgtagaaatt tcctttaatg tagatggttt tgaagccaat tggtgataat cattgtagca
     9001 cctgaattgg atttcacaac attttcgcag attgcaccgg cttgttccct ctttttattg
     9061 ttgtttaaca aatcatcaaa aacagttttc atctcagtaa ggttattaat agtaaatgct
     9121 ccccctgatt caatcaacag tttagcttct gcaaactttt gaaaattagg accaaaaatt
     9181 acgggcttgc catatacagc tgcttccaat gtgttatgta ttcccttacc aaagccacca
     9241 ccaatgtagg ttattgttgc gaaacgataa attttagaaa gcataccaat cttatctata
     9301 atcaacacat cggctttttt taattcttct tttgtagcct tactgtggcg caccacattt
     9361 tttctacatg cataagtaaa atctttgtat gaactttcat ggatttcatg cggcaccaat
     9421 agcaatttta tctcttcgga attacaataa ccggccaaca acttatgatc ttccggccag
     9481 gtgctgccgg caacaataat tctttcacct actgaaaaac tttcaatctt ttcgtctgta
     9541 aattccaatt ccggcagttg aatcaccctg tcaaaacgtg tgtctccact cacttcaacc
     9601 tgagttattc ctttgttatt taaaatattg agagaggtat cattctgaac aaaaaacctt
     9661 gacgcttttt taagttcttt aagaaatgca cctccaaacc atttgaaaaa aaactgatca
     9721 gttctaaaaa tagcagagat gtaaataaat ggaatgtgtc tcttgctcaa cccgtttatg
     9781 tagtgaaacc agaaatcata ctttacaaat atggccatgt gcggatttat gtaatccaac
     9841 catttatgtg cattagtgct actatcacat ggtaagtaaa aaataaaatc agccaggttt
     9901 tctttaatgc cctgcttata acctgacgga gaaaaaaaag taagtactat tttatattgt
     9961 gggaaaagtt tttttatctg ttgcaaaaca ggtttgccct gctcgtattc gccatacgaa
    10021 gaacaatgaa accaaatgcg ttttgcagtt tcatttttaa gttcttggtt tactttttga
    10081 aatatatttc ttcgtccttc agtaaaagct tttgctttat cattaaaagg tgaagcaagt
    10141 gcagctgcta aatgatagag tcttattcca atggaataaa gaagtagcat gattaatagt
    10201 aataaaattt atcggggttg gtatagaaag gaagaatcca accggcttta aaaccataca
    10261 acaaatcaac acgactttga tcatcggctt tcatggtatc aaactgataa tctctgcgac
    10321 cttgtgtaaa cccctgaatg aagttaaatc ctgcataaaa gtttatcagc tttttatttc
    10381 cgtaataaac atatccgata aattcttcaa ctataattcc gttggtaagt ctgtcataac
    10441 cttttttgta ttttttagaa agccctttaa cagtattgcc aatggtttca attctgattt
    10501 tatgttgcat aaatcctgca cctgcactta tccaaattcc actattggga tttggctttt
    10561 taaatgcaaa cacttttcct gcctgtgcac tgatgtgata tcctctttca aaaagtctta
    10621 catcagctgt acggccatca ctgccgatta tagtaccata acttgatgca atgcctgcaa
    10681 gcaatccggg ttgattgatt ttatttccaa aaaaaaatcc accttgaata ctgtaaagaa
    10741 aattctttgg tgtcttaaca ccaaagacgc cttgcactcc ataatcagcg ccaaaacgtt
    10801 ctttcatatc accacctggt aaatgaaatt ctcctcctat accaaaaaat ggaatgacaa
    10861 tagcagagtc gcgtaccaaa gcctgtgcag cagtctgtaa tgaagaaaaa gaaatcaata
    10921 tggctaatag aaatacttta acctgcattt taatcaagtg gaattaaaag acgcttaatt
    10981 tttggtaccg gtcccggaca atgactgctg tgacaagcca gacatgcatt tacaacgtta
    11041 ttgaaacttt taactctgtt tgtattggtt gcattttcat acgaatccaa agtctgaaga
    11101 tataagtcgg caaatgttgt gtaatactca ttttttgttt tatcatcagt tggctttgcc
    11161 gtattgatgt ttaaaaatgt ttttgggtag gaaactgtcc gttgttcact tacagccttt
    11221 cgtgcttctg cagcatggtt gtacatctgt cgcatcagca gtgaaagctc actgctttta
    11281 ttgggattaa tatctgttgt atcagcaaaa acttcgtttg tccatcctgt tttgtagtga
    11341 taggatgaaa cagaaagcat gagcattatg cttgctatga caaatatttt attcataaat
    11401 aattactcct tctgcttcaa aagcaggctt tttttctggt tgggtaattt tctcaatttc
    11461 ttcttttgat ttattggcat cctctgcata gtgacgaagt tctgctactg atgtcacatc
    11521 attaaatgct ataccttcaa tgattgcagt ttttcctgca caatctttag gcacaaaaaa
    11581 ttcatagtct ttaaaacgaa cacgaatggt atgcccatct gatgtttcca tcatcatcca
    11641 gcagcctttt ttctgacaga cgctggcaat tttgccctga attttaacat tcacggtgtc
    11701 ttttgtagtc agcattctgt caacaacaga agcaggttgt gcattctctt cagtgatttt
    11761 ttttccgaaa tgagacgctt ttttatcctg agccatcaaa gacagactca taacagttgc
    11821 aacggcaacc aatatcaatt tacgcatgtg attgaatttt gaattgcaaa ggtaattttt
    11881 tgatttaatt aacatacttt attttacatt ctatagctat aagtccaact tttcattaaa
    11941 caaacatcct gaagaaggat cttcgggtaa aatttttcta acataaatct gttatttact
    12001 ctgtaatata aaagcaaaaa ggttacttta tgaaaatacc tttgtctgat gtttttaaag
    12061 agatgttttt ggtgtttgct aatggtgttg cttccttttg tttcgtattc gcagaagaag
    12121 ttacccggat ttattaaccc tgtatcctat cattgggccg atagtgtgct tgatgaaatg
    12181 actgttgacg aaaaaattgg tcagcttttt atggtacaaa tgcaaagtgt ttggcctgaa
    12241 aaagatttag attcattaga gtcattaatc cgtaattatc atctcggagg gttaattgtt
    12301 tttaaaggag gcccttaccg tcaggcactt tgtattaaca gaatgcagca ggcagctaaa
    12361 attcctttgt tggtgagtat tgatggcgaa tggggactta gcatgcgcat ggacagcacc
    12421 attcgttttc cacgacagat gaccttgtct gccatacatg atgacagttt gcttagtgat
    12481 gcattttatg aaattgcatc acaatgtaaa cgcatgggta ttcaactcaa ctttgctcct
    12541 gatatagaca tcaacaataa ccctcagaat cctgttatta atacacgctc atttagtgat
    12601 gataaatttg aagtagcaga aggtggaatt ctgtatatgc aggctttgca aaatcagcat
    12661 gtacttgcat gcggtaaaca ttttcccggc catggcgata cagatacaga ttcacattat
    12721 gccttacctg tgattaataa atctgccaac caattagata ccttagagtg gttccctttc
    12781 aggcagctta ttaaaaacgg tgtcagcagt atcatgacag gtcacctgaa tgttccatta
    12841 cttgacgatg agaattatcc tgcttcatta tctaaaagaa ttatcaacgg ctatcttaaa
    12901 gacgacctcg actttaaagg actcgtgatt actgatgcat tgaatatgaa aggtgccatt
    12961 ttagctgatg ccaaaccggg tgatattgag ttgcaggctt tgttggcagg taatgatatt
    13021 ttattgatgt ctgaaaatgt gaaaccggca attgaaaaga taaaaacagc acttcaaaaa
    13081 agaaaattac gtaagagtgc tctcgatgaa aaagtcagaa aaattctgat ggccaaacat
    13141 tgggcaggtt taaccaatta tgctcccatt gatactgcta acctttatta tgatttaaac
    13201 aatgcagatg cttcatatct caactaccgt ttgtatgaaa agtcgctgac tttacttcgc
    13261 aataatgctg ttttgccact actaaacagt tcagcagagc gcatggcatc attagtaatt
    13321 aatgatacgc ttgaaaacac ttttcagcaa acattatcac tatttgcacc tgtacagaat
    13381 tttgtcatgt caaaagatgc atctcctgcc accgtggaca gtattgccgc agttctttca
    13441 gggtttacaa aagtgattat cagcattcac aatacaacaa ccaagcctca gtttaatttt
    13501 ggtattactg aaagcatgaa ttcggttatc caaaaagttt ctatgcgcac caaagctgtt
    13561 gttgtgttgt ttggaaattc atattgtctg aataaattgg ttattcccga tcagactaaa
    13621 gcactgataa ttgcctatga agacacctat ctgccacaac acataactgc acaggcgttg
    13681 tttggtgcag ttccttttaa tggcagattg cctgtatcac cgggtttatc ttttcatcgt
    13741 ggtgatggtc ttaaccttca ggcaaccggt gttataaaat ataccatgcc tgcagagtta
    13801 aatattgatc ttacttgtct gcaaaaagtt gactccatca taaacgatgc aataaagcag
    13861 aaggcttttc cgggatgtca ggtatatgca tcggttgacg gcaaagttat ttataacaaa
    13921 tcgtttggtt atacaactta tgcagataca caggcggtgg agaatgatat gatttatgat
    13981 attgcctcca tgacaaaaat tgatgctact gcacttgctg ccatgtggct ctatgaccat
    14041 catctgcttg atttaaaaca gactgttgca tattatttgc ctgattacga aaagacaaat
    14101 aaagggtcat taacagtaga gcagcttatg acgcatactg caggacttca agcatggatt
    14161 cctttttata aagaaggact taccaagaat ggtacgatga tgaaaaaata tttttcttcg
    14221 tcaaagcatg atgactatac tgttcagata actgatagtc tttttatgaa tcctaaattt
    14281 tattcaaaaa tagataaaca gattaaagaa tcgcctttaa cagaaaaagg aaaatatgtt
    14341 tattctgatt taggaatgtt gcttttgcaa aagattattg aaagacaggc aggaaaaaag
    14401 atggatgtat tgctcaacga agaattttat caaccattgg gtttaagtca tttgttattt
    14461 aatcctcgca gtaagttagc attaaaaaat attgtaccta ctgaatacga tacagtgttc
    14521 agacaaacat tggttcatgg ttatgtgcac gatcctgcag cagcattgtt tggaggtgtg
    14581 gccggcaatg caggcttatt cggcaatgcg cagagcgttg gtgtactcat gcaaatgctg
    14641 ctcaatgaag gtagctatgc tggcaaacag tatataagta aggctactgt tgatactttt
    14701 acagaagcac ctattcccgg aataagaaga ggcttggtgt ttgataaacc cgaaacaaat
    14761 ccggcaaagg gaagtcctac atgcaaaagt gcatcaccgc aaactttcgg tcatcaggga
    14821 tttacaggta cctgcacctg ggccgaccct tccaatcagt tggtttacgt ttttctttct
    14881 aaccgtatat tcccttctgc tgcgaatcag aaaatcagta agctaagtgt aaggacggaa
    14941 atacagcaag tgttttacga ttgtttgcgg aaagtacgtt gaaagaaact atttttacaa
    15001 cctgttaaac aattcaacaa tttgcttgtt gattgattct tacagtatga aaataggaat
    15061 agtttgttat cccacttttg gtggcagtgg ggttgttgcc acagaacttg gcaaagcact
    15121 tgctgctaag ggtcatcgca ttcactttat tacctacagt caaccggtac ggctcgattc
    15181 tttttctgaa aatatttcct accacgaagt atcagtaagc aactatccgt tgtttgaata
    15241 tgccccttat gagtcggcac ttacaagtat gttggttgat gtggctatgc acgaagggtt
    15301 agatgtgttg catgtgcatt atgccatacc gcatgcatca gttgcatatc tggctaagca
    15361 gattttaatt tcaaaaggaa tttatattcc tgttatcacc accttgcatg gaacggatat
    15421 tacacttgtg ggccgcgata atagttatga gcccgttgtt acgtttgcaa ttaaccaaag
    15481 tgatggcgtg acagcagttt cagaaagttt aaaacgcgat acctatgcaa attttgaaat
    15541 caccaaacac attgaagtca tccataattt tattgatctg aaaaggtttt caaaaaaacc
    15601 taaagaccac ttcaagcgtg caattgctcc cggtggcgaa agactgttgg tacatacttc
    15661 taatttcaga aaagttaaaa gggtgaatga tgtgatacag gtttttgcgc gtattcataa
    15721 aagaattcct tcgcggttat taatgattgg tgatggtgat gaacgtgccg gagcagaaaa
    15781 actgtgtcgt gagttacaac tctgtgatga tgtcaggttt ttaggcaagc aggacgctat
    15841 tgaagaaatt ttatctgtgt gtgatttatt tattatgccc agtgaaactg aaagttttgg
    15901 tcttgctgca ttggaagcta tggcatgtca ggtgcctgta atttcatcaa acaccggtgg
    15961 tatgccggag ctcaacttag atggtgtgac aggatttaca agtaatgttg gcgacattga
    16021 agatatggct gagaaagctt atcacatttt aaaagacgac cagacactca atgttttcaa
    16081 gcaaaatgct ttgaaacgag cttctgattt tgatattaat gtcatccttc cgcgttatga
    16141 aaattactat cagtccgtaa ttcagaattt tactgttgct tcataaattc cggcactttc
    16201 acgtgttttt tcttgaatac tgttgtcgtt gaataatgaa aattgatgtc cggaagtagt
    16261 cctttagaac tcattttaat gatttgttgt gaataatgtg gcaaaaaaaa tctgcaaaag
    16321 ttagaaacta aattactgat aatcaatgat attttaaaaa catagcagat agaaacagaa
    16381 aacggttgaa taatattggc aaactgttca cactattttg tttataacgt ttgttggttt
    16441 gaaaatttgt gttacttttg cgaccccaat tgtaaaaggt taatacataa tactgtggat
    16501 actttatcat acaaaacaat atcgctgaat gcgaaaactg ccaagaaaga gtgggtgctg
    16561 attgatgcaa ccgatatggt tttaggccgt ttatcatccg aggtggctaa gattttaaga
    16621 ggcaagcacc gtccaggatt tacgcctcac gttgacaccg gagataatgt aattattatc
    16681 aatgccgaga aggtaaaact taccggtaaa aaaatgacac agaaggtata tgtgcgccat
    16741 acgggctatc ccggaggtca gcgttttacc tcacctgaaa aactgatgga tacgtatcct
    16801 gaaagaattc tggaaaatgc cattcataaa atgcttccta aaaacagatt gggaaatgct
    16861 ttaaagaaaa acctaagggt atttgctggc agtgagcaca gccaccaagc acagaatccc
    16921 cgtaaaataa ctttagatat ttaatcggca atgaataata tacataaaat aggaagacgc
    16981 aaaacagccg tagcgcgtgt ttatctgact ccaggaacag gaactattgt aatcaacaat
    17041 cgtgaattta aaaactattt cactaccggt ccgcttcaga ataaagtaac acaggcattt
    17101 gcagttatca atgcaaacga gcagtttgat gttaaagcca atgtgatggg tggcggaata
    17161 accggacagg cagaagctat tcgtttggcc atttcacgtg cattgtgcga aatgaatcct
    17221 gaaaaccgtc cggctttgaa aaaagaagga ctcctgacac gtaacccaaa aatggttgag
    17281 cgtaagaaga gcggtcagaa gaaagcccgt aagaaattcc agttcagcaa acgttaaaaa
    17341 attataccag caaaaaatgt ctattaatac acaggaattg ttagatgcag gttgtcattt
    17401 cggacacctt aaacgtaagt ggaatcctaa aatggctcct tatattttta tggagcgcaa
    17461 tggaattcac attattgatt tgaataagac agctgctaaa cttgaagaag ctactgctgc
    17521 cataaaacaa attgcaaaat caggcaaaaa aatattgttt gttgctacca agaaacaagc
    17581 aaaagaaatt gttgccaatg cagccaagtc tgttggcatg ccatatatca ctgagcgttg
    17641 gccgggtggt atgcttacca acttcgcaac acagcgtaag tcagtaaaga aaatgacagg
    17701 ctttgaccgt ttcatgaaag acgaatcttt caacaacatc tctaaaaaag agcgtcttca
    17761 ggtatcgcgt cagaaagcga aaatggaaaa attaatcggt agtattgccg accttacccg
    17821 tttgcctgct gcactattta ttgttgacgt attaaaagag catattgcag tttcagaagc
    17881 acagaaatta ggtatcccta ctattgctat ctgcgacacc aatacagacc caactttgat
    17941 tgacttcccc gttccggcta atgatgatgc aacaaaatca atctctttaa ttatgaatgc
    18001 catgattaag gcaattgaag aaggccttga agagcgtaaa atggacaaag aagcagaccg
    18061 cgagcgcgaa gctgctgctg aagaagaaag tggcgaaaga ggtgatggcg aaaaagaatt
    18121 tgtaatgtca gaagaaggtg atgattcagg cgaaggaaaa tcaaaagctc ctgctaaaaa
    18181 agctaccaca cgcagccgta agccgaccac aagaaaaaaa gattaatatt tttaaagatt
    18241 tataattaca ataatgagta cagtaacaat tagtgcaagc gatgtaaaca agctgcgtca
    18301 gatgaccggt gcaggaatga tggattgcaa aaaagcactg atggaagcca atggcgattt
    18361 cgaaaaagca attgatgaac ttcgtaaaaa aggacagaaa gtagcagcta acagagctga
    18421 ccgcgaagca aaagaaggat atatcgttgc caaaacaaat aacgatgcta ctaaaggata
    18481 tttggttgca ttaagctgcg aaacagattt tgtagctaag aatgcagagt ttgtaaaatt
    18541 tgcagaagat gttcttgaaa cagcattgac aaataatgca gcaaatcttg atgcattgtt
    18601 ggcattacaa ttacaaggca caacaatcag cgagaaattg attgagatgg ttggtaaaat
    18661 tggagagaag attgaaatta cccgttatgt agttatcgaa gcccccaagg tggtagttta
    18721 tattcacccc ggaaacagag tagtttcaat ggtaggtcta tcatctgcta cggctgctca
    18781 tgaagccggc aaagatgtgg ctatgcaaat tgctgccatg aatcctgtag ccatcgacaa
    18841 agacgatgta gatcaaagta ccttggatcg cgaaatcgaa atcggaaaag aacaggcacg
    18901 tgcagaaggc aaacctgaaa atatgattga gaagatagca atgggtaaac tgaataaatt
    18961 ttatcaggaa tcaactttg
//
LOCUS       DAOQNP010000063        18947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1024_length_18947_cov_11.101630, whole genome shotgun
            sequence.
ACCESSION   DAOQNP010000063 DAOQNP010000000
VERSION     DAOQNP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18947
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1024_length_18947_cov_11.101630"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKY09_09145"
     CDS             <1..377
                     /locus_tag="PKY09_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13063.1"
                     /translation="QTYSATLAGQYKVIETANGCYKTKSAQLAVTSCREEGEGIIANK
                     QSNISISPNPFSDLLNIYAPSEKEGNAEIQVLDMVGKVVYRSVITLNQTSEIKLNVPA
                     GIYFLSVNVDGRHLIQKIVKSR"
     gene            492..929
                     /gene="ssb"
                     /locus_tag="PKY09_09150"
     CDS             492..929
                     /gene="ssb"
                     /locus_tag="PKY09_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB13064.1"
                     /translation="MSGVNKVILVGNLGRDPEIRHIDGNISVAKFPLATSEVYKNKDG
                     QRVTQTEWHNVVLWRQLAETAEKFLKKGSLVYIEGKLRTRNWDDKEGNKKYITEVIGD
                     SMTLLGRKPDENGNPSTGANDRTTSEHHIPDAGQSEMNDDLPF"
     gene            997..2307
                     /gene="gldE"
                     /locus_tag="PKY09_09155"
     CDS             997..2307
                     /gene="gldE"
                     /locus_tag="PKY09_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HNB13065.1"
                     /translation="MPVVSSLLLTNFSSYSFTGELIVILVLLLLSALFAGSEVAFFSL
                     RPAQLQYLRDEKPTYINKIILHLLDQPKKLLATILISNTCVNIALIIFSSLFFSNSQL
                     LGDNPILIFFVQVFSVTFLITMFGEVLPKVYAAHNNLRILSFMAYPVYIIGKILTPLS
                     VPLVKSTSLFDKLLKRKRTNVSLDELTHAIEITSDIESPPEEKRILKGIVNFGNIDVK
                     QIMRPLPDMIAFGLQTPFKELLQRVIDAGYSRVPVYESSIDNIKGVLHIKDLLAHIKK
                     ENFDWPLLIRKTYFVPENKKINDLLQEFQQRKIHMAIIVDEFGATQGLITLEDVLEEI
                     VGEIKDEFDDEEPVYTKIDEQTYVFEAKTSLNEVCRILELDMEMFDEIEGEKDTLAGL
                     LLEINHTIPRISEVITFKNLRFTIESADRRKIKRVKLQIIQEQE"
     gene            2304..2870
                     /gene="gldD"
                     /locus_tag="PKY09_09160"
     CDS             2304..2870
                     /gene="gldD"
                     /locus_tag="PKY09_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNB13066.1"
                     /translation="MKKLVYIFLFCLLASCSETPAPKPRGFFRIDMPEKKYTLFTDTS
                     CPYRFETPQYSYVLPDTNRFAEPCWKYILYPKFGAQLYLTYKPLKNNLAQMAEDSRGF
                     AYKHTIKANGIDENVVKTQNNVYGVVYDIGGNAASNMQFYVTDSLKHFLRGSLYFNTI
                     PNADSLQPSLEFIRKDVLHLINTLQWSN"
     gene            3235..4611
                     /locus_tag="PKY09_09165"
     CDS             3235..4611
                     /locus_tag="PKY09_09165"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HNB13067.1"
                     /translation="MPKHSWAEPFKIKMVELLKMTTPQQRKKALQQAGYNTFLLKSED
                     VYIDLLTDSGTSAMSDQQWAGMMLGDEAYAGSKNFYRLEEVVKKYYGFKHLIPTHQGR
                     GAENILSQIMIKKGDVVPGNMYFTTTRLHQELAGATFYDVIIDEAHDSSNLHPFKGNI
                     DINKLDKLVKKFGANKIPYVCVATTVNMAGGQPISMANIKELRKYTAKLGIKVMLDMT
                     RIAENAYFIQQREEGYKRKSIATIVKEICSLTDGATFSGKKDALVNIGGFLALNDDKK
                     FEEASNLVVVYEGLHTYGGLAGRDMEAMATGIIESVNDDHIRARIGQVLYLGDKLNEM
                     NIPIVNPVGGHGIFIDAKKFLPHLKQTQFPAQALAAEIYEDSGVRTMERGIVSAGRNK
                     KTGDHYYPELELVRLTIPRRVYTQAHMDVISESIERVYKNREKIKGLEMIYEPTYLRF
                     FQARFKKR"
     gene            4876..5724
                     /locus_tag="PKY09_09170"
     CDS             4876..5724
                     /locus_tag="PKY09_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13068.1"
                     /translation="MLNRIVGIFTAALTFTLLVTSCSSPPEEKKEATTVAAKTAATVA
                     PLENDHLLSVNNAVLLIKNYLDTINNSENAEALRKLPVGGSISAEALKRMTEENPDAN
                     GYMFYGFNDAEPATLFKEAKMLAACKFNFNPDSMVSYETNDATNVYLINDTVKHTFNS
                     LTSEAITNYLSQYNKVIPSAASVYHYSNIKGNMSQYTSFFNENFGDGTVQWPYALFSS
                     DEFLTRLYEATEGESNFGGIRYYFGLDKEAKENKLRIIIFAVTKSGTNVVVDNKILER
                     HWPPSS"
     gene            5729..6397
                     /locus_tag="PKY09_09175"
     CDS             5729..6397
                     /locus_tag="PKY09_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13069.1"
                     /translation="MDHFYTRMLATCIEVLVALFGVMIFKKAGLQFRLLIFLLLYGAA
                     TDIFGWTFYKLNRTYVLIAVDIYTLAEALLMVFIIQSIGLFNNLKNIFKLIYLLLFVC
                     YVGAYFVMPLISDDGAYRGPYSFGYLSTVAVLAAWASLKWIEGAGNKLNSPVFALLAG
                     VFIYTFCSVFIDSFIGTTTGDAVWWIHDAANITAYLIFAYCFYLIKKSTTVEKSVINL
                     PEKV"
     gene            complement(6375..8714)
                     /locus_tag="PKY09_09180"
     CDS             complement(6375..8714)
                     /locus_tag="PKY09_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB13070.1"
                     /translation="MLSYIFRIPNRMYKQLFIAFFLLLSATQTYSQNLPLGSWREHLS
                     YTQAQFVSNTPQRVYCATDQAVYFIDKSDNSVQRYSKINGLSDVGIRLMDYSHTQNVV
                     FVGYVNSNIDLISSTGIINISDVERRTATGDKTINDVAFNGRFAYLATGFGVLALDLI
                     KAEIKETYVVGTTGNEVPVYSVTTDGTYIYAAIAGGILRGEISNPSLNNFATWQMVYP
                     NTFTGVTFNKMCWLNGNLAVNFHSTSTDSVVIVNTTNFSTGTTLLSGGFALRQLKAEN
                     DLLYAVNNYSVNEWDNNFQPLRQIYGNANYQNTDMFGVATGSGNQLYIADRNNGLVNY
                     TDNNNITFTVPNSPASPNASCVFHNGTTLVTTHGTNRWSDSYGNDGYSTFDGVQWKTW
                     NVKTIANSNVDLNQMLDLQAVVSEPGNPGHFFLGSRINGVFEFDNNVPVKMYNKDNSS
                     LKGAFGNPGQTKIGGLAYDAAGNLWVVNSGVNTPLNVLSKEGVWQSINLQSVIPSGVN
                     IFFGDILCDSYGQVWTTLISNGLIVYDPVAQASARISQETGGLNVNDVRCMVEDKEGQ
                     IWLGTSEGIAVIYSPGNATGSTPAKAQKILITIDGITQYLLDKEIITAIAVDGANRKW
                     IGTNGGGVFLLSADGTKQIQNFNTSNSPLFSNAITSIAIDPVTGEVFIGTDKGLISYV
                     GDATQGTESCSGLKVFPNPVKENYDGPIAVRGVTANGNIKITDIAGNVVYETTAKGGI
                     ATWDGNNFEGKRAQTGVYLVFATDEKGNNTCVTKLFLVN"
     gene            8791..9933
                     /locus_tag="PKY09_09185"
     CDS             8791..9933
                     /locus_tag="PKY09_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNB13071.1"
                     /translation="MALIAVNTRMLIKDKLDGTGWFAFETLKRIVTQHPEHRFIFFFD
                     RKFNAEFIFSSNILPVVIYPQARHPWLWYTWFEISIHRALKKYRPDLFFSPESFICSK
                     AAVPQVAVIHDLNFEHFPQYMPAQVARYYSKWSRIAAQNAKRIGTVSEFSRQDIVKTY
                     NINIEKTDVLYNGIHDLYKPLSAAEQEQVRNQYTLGCRYIIYTGSLHQRKNTANMVRA
                     FAQYKKKYGGELKFVLVSDRSKMEGDLQLALSEIGIEKDLIFTGRLSAKALAQLTASA
                     EAMMYVSFFEGFGIPVIEAMRCGVPVITSSVSCLPEISGNAALCVDPHSVNAITGAME
                     QVLQHQEFAEQLKQKGKIRQQDFSWDKTASLTWQSLQLVLAEKGKE"
     gene            complement(9883..12015)
                     /locus_tag="PKY09_09190"
     CDS             complement(9883..12015)
                     /locus_tag="PKY09_09190"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNB13072.1"
                     /translation="MINFLKKYPYVLLVLIAVVGYYQVALFQNTLKWDVTDQYYPWRM
                     FVVQCLRNGILPFWNPYEQFGYPMHADPQSGVWYPVVWLMSLFGPYTLYSVQLEFLLH
                     VIGGGVGMLFLLQRFHVNPFIALINAIAYMFCGLFVGNAQHLTFTIAACWLPFVILAF
                     KNMAEHNHWRFALHFALFGYLLVSGGYPAFAIILFYILLTVTVFLFIRNRQSKHWMKR
                     FLLHSIFAVVLLLLCSSGILYSVFQSSDYLARATGLSFKVASFGPFSPQSLISLMFPL
                     ASARDSEFFGTDISMSSVFIGMLPLAGFMLFIIRPKSRSAWLILAATIVMLFASFGSY
                     TPVFGWLYKTFPMLDLFRFPSVYRLFFITGILLLSGIALSAPDAFSRLKLPLVTLGCI
                     AAVVTIFFWFKNTDDWNFKPWHDAVNNISFTQAIAFQGFIQLFFIVMMSLTLLIKDRA
                     KLLMTIFTLSAANVVVAAQLNIPVTVTYNFKTSVVAKEVKEKAIADFPVPEVRPVVLN
                     NDSGGWVSPFWRNLNLFKKQPAWDGYNSFQLKNYVRFVDDESLRQRQITNNWIYFADT
                     VIAYLEEPIIVAPASVHNVCYVPASEKYLLNIQPTAGNTVSVKTFIPGKIIVEAKAET
                     SSALTLMQQHYHGWKIYDNGKEVTPVVTDYLFMTIPVAQGSHRIEYRYENKPIHYALW
                     LSGVTLITLIGFSLFAFFSKNKLQTLPR"
     gene            12117..12485
                     /locus_tag="PKY09_09195"
     CDS             12117..12485
                     /locus_tag="PKY09_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HNB13073.1"
                     /translation="MSVKLLTDYLQQHTLIGIKSGLHRKSFTDIWMVTVEGRFFSRSW
                     NKSHDGWFGEFLKNGAGQLKLNNIILNVHVQKIEKDDVVQQKIDLAYLSKYTQPENAS
                     YAHGISHPDYHDFTLEFFPE"
     gene            complement(12499..14037)
                     /locus_tag="PKY09_09200"
     CDS             complement(12499..14037)
                     /locus_tag="PKY09_09200"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNB13074.1"
                     /translation="MATTDDLFKNIISHAKEYGFIFPSSEIYDGLSAVYDYGQYGVEL
                     KKNLRDYWWKAMVQTNPDIVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEEHVAKIEDKIRKEVEKAAKRFENFDEAMFLSTNARVLEYRQQADKINARFKEA
                     LEGGNLTEVRQIIIDLEIVCPVSGSRNWTEVRQFNLMFSTQLGSVTDDAATIYLRPET
                     AQGIFVNFLNVQKTGRMKIPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQYFVKPG
                     TEMEWYNHWKEKRFNWHLSLGLGAERYRFHDHLKLAHYANAACDIEFRFPFGFKELEG
                     IHSRTDFDLSNHEKYSGKKLRYFDTEQNTSYIPYVVETSIGLDRLFLAVLSTAYNEET
                     LNDGSQRTVLNIPPFLAPIKCAVLPLLKKDGLPEVAQNIFNTLRFSYQCHYDEKDAIG
                     KRYRRNDAIGTPFCITVDHQTLTDNTVTLRHRDSMEQQRIGIKELKAILDKELALENI
                     LAKL"
     gene            complement(14133..14543)
                     /locus_tag="PKY09_09205"
     CDS             complement(14133..14543)
                     /locus_tag="PKY09_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB13075.1"
                     /translation="MKSSSPSRLQHVLLIDDNEIDNFINERIITSSGFSEVCTVKTSA
                     DEAIEYLNTIAKDSKVPLPQVIFLDLNMPVKDGFAFLDEFNTLAENIRQSCRIIVLSS
                     SISPDDINRASANAYVIRYLNKPLAEKYLEAIDI"
     gene            complement(14568..14640)
                     /locus_tag="PKY09_09210"
     tRNA            complement(14568..14640)
                     /locus_tag="PKY09_09210"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14605..14607),aa:Phe,seq:gaa)
     gene            14844..15365
                     /locus_tag="PKY09_09215"
     CDS             14844..15365
                     /locus_tag="PKY09_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13076.1"
                     /translation="MHDFINKERLFALLTSLIFILLALPGFCVSSEPEHEHHPHKAEI
                     GIGNSAVYFTKDKTLNYGFHFHFSRNIKHSNFSVGAEYERIFDRHGHNMIGLVAGYSP
                     AHRVELSITPGILFEDAEPNHADVSLHFEAGYHFDIKNIHLGPIVECAVDRHDVHFSI
                     GLHLGIGIGKLIE"
     gene            15674..16111
                     /locus_tag="PKY09_09220"
     CDS             15674..16111
                     /locus_tag="PKY09_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNB13077.1"
                     /translation="MKERFNLSKADPKAFDAMLNLEKYLAESGLDKILFELIKTRASQ
                     INGCAYCINMHVRDAMKIGETSQRLFLLDAWRETTLFTEKERAVLALTEAMTLIANNP
                     VSDAVFDDAKNHLTNKEMAAVMMTIVTINAWNRIAITSRTALD"
     gene            16170..17609
                     /locus_tag="PKY09_09225"
     CDS             16170..17609
                     /locus_tag="PKY09_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB13078.1"
                     /translation="MDSQSIIEELKKLNKISSQLDISQEERASLLNQTAEYTNNFIST
                     LHTIKGYQEKIVGNLSINNKPASLKDLLTLYQHEVAESGINAASPKHLGYIPGGGVYT
                     AALADFIAAITNPFAGAYFAAPGAATIENEVVDWLKKVFSFPESAVGCLSSGGSVSTL
                     IAFTAARDKFKIKNEVIPKSVVYLSEQVHHSTQKALRIIGLEDVIVRYIPLDDWHKIK
                     PDALNALVEKDKAEGLNPFLIVATAGTTDTGTIDPLNDIATIAAQQKLWFHVDAAYGG
                     FFILTSKKNLFKGIERADSIIVDPHKGLFLPYGVGAVLVKDSNAVLHSHYYTANYMQD
                     GQNEELLKSPANLSPELTRHFRGLRVWLPLKYHGIEPFIACLEEKLLLVQYFRMKLAA
                     RGFCLGPEPDLSVSYFWYPFANDADEKNKLLMEEIHKDGSVFLSSSIINNRFVIRIAI
                     LAFRTKKETIDEAVEMIDRCLENILSNYS"
     gene            17737..18444
                     /locus_tag="PKY09_09230"
     CDS             17737..18444
                     /locus_tag="PKY09_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13079.1"
                     /translation="MKKTSLQQDNSLETMKSSSKKKTRFNLKTHSVFLLLLCLGTLVT
                     SCKKDNDSNPLESTQYYSLQDGMRKLWNDHMQYTYATVDAYFNNPSGLSSSLNRLLEN
                     QKDIGAAIVPYYGQAAGDTLSALLTTHINLAVPVLEAAKNNDQPALNQALANWKTNAN
                     DIANFLSTANPKNWPPHDMQHMMEMHIDQTTTYAVDLLNKDYNNAITHYDEANQHMLD
                     MADDLAKGIALQFPDKF"
     gene            18474..18848
                     /locus_tag="PKY09_09235"
     CDS             18474..18848
                     /locus_tag="PKY09_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13080.1"
                     /translation="MENKILSQTFNLLKYTFVVVPIVAGADKFTNLLTNWPQYINPTI
                     AGMLPLTPAVFMMIVGVIEIAAGIIVLKKPAIGGQIVAAWLTLIALTLLLGFNYIDVA
                     VRDLVMAISAFSMAQLAKFVKQ"
BASE COUNT         6166 a         3550 c         3496 g         5735 t
ORIGIN      
        1 cacaaactta ttctgcaaca ttagcaggac aatacaaagt aattgaaaca gctaatggtt
       61 gttataaaac aaaatcagct caactggcag taacttcttg tcgtgaagaa ggtgaaggta
      121 taattgctaa taaacaaagt aacatcagca tatcgccaaa tcctttttca gacctgttga
      181 atatctatgc tccatcagaa aaagaaggca atgcagaaat tcaggttttg gatatggtag
      241 gtaaggttgt ttatcgttct gttatcacct taaatcaaac atcagaaatt aaactgaatg
      301 ttcctgcagg aatctatttc ctatcagtta atgttgatgg aaggcatctg atacagaaaa
      361 ttgtgaaatc aagataataa agtcattcat tcaaaaagac ctcgcttctg cggggttttt
      421 ttatgaccaa attgctatat ttgtaagcat aaacaaaaac acattcatta cgtagttaaa
      481 taaaagcaat catgtcagga gttaataaag taattttagt aggtaacctt ggaagagatc
      541 cggaaatacg tcatattgat ggcaacatca gtgtagcaaa gtttccattg gcaacatcag
      601 aagtttataa gaacaaagac gggcaaagag ttacacaaac agagtggcat aatgtggttt
      661 tgtggcgaca gcttgcagag actgcagaaa aatttttgaa gaaaggaagt ttagtatata
      721 ttgaaggaaa attacgtacc cgcaactggg atgacaaaga aggcaacaaa aaatacatta
      781 ccgaagtcat aggcgacagc atgacattac ttggtcgcaa gcccgatgaa aacggaaatc
      841 cttcaacagg cgctaatgac agaacaactt ctgaacatca catacctgat gccggacaat
      901 cagaaatgaa tgatgacttg ccattttaag tgcatgccca gctaagcaac atcatcctat
      961 atttgcagta aattatattg tattcacccc atcagcatgc ctgtcgtcag tagcttgtta
     1021 cttaccaatt tttcatccta ttcatttacc ggtgagctga tagttattct tgtgttgtta
     1081 ttactttctg cactttttgc aggaagcgaa gttgcctttt tctcacttcg tcctgcacag
     1141 cttcagtatc tgagagatga aaaaccaacc tatatcaata aaataattct acacttactt
     1201 gatcagccta aaaaactact tgctacaatt ttaatttcca atacctgtgt aaacattgca
     1261 ttaattattt tttcttctct ctttttttca aactcacagt tgttgggtga taatcccata
     1321 ctcatttttt ttgttcaggt tttcagtgtg acttttctta tcacaatgtt tggtgaggtg
     1381 ctacctaagg tttatgcagc acataacaac ttacgtattc tttcctttat ggcttacccg
     1441 gtttatataa ttggaaaaat acttacacca cttagtgttc cgttagtaaa atcaacatcg
     1501 ctgtttgata agctccttaa acgtaaacgc accaacgtaa gcttggatga attgacacat
     1561 gccattgaaa ttaccagtga tattgaatct ccaccggaag aaaaacgtat tcttaaagga
     1621 attgttaact tcggaaatat tgatgtcaaa caaattatgc gtccgctacc agacatgatt
     1681 gcttttggtt tgcaaacacc atttaaagag ctgttgcagc gtgttattga tgccggatat
     1741 tcaagagtgc ctgtttatga atcttctatt gataacatca aaggtgtgtt gcacataaaa
     1801 gatttattgg cacacattaa aaaagaaaat ttcgactggc cgcttcttat ccgtaaaaca
     1861 tattttgtgc cggagaataa aaagataaat gatttattac aggagtttca acaacgcaaa
     1921 atacacatgg ctattattgt tgatgagttt ggcgcaacac aagggcttat tacattagaa
     1981 gatgtgcttg aagaaatagt aggagaaata aaagatgaat ttgacgatga agagcctgtt
     2041 tacaccaaga ttgatgaaca aacctacgtg ttcgaggcaa aaacatcact gaatgaagta
     2101 tgccgcattc ttgaattgga tatggaaatg tttgatgaaa tagaaggaga aaaagacacc
     2161 cttgcaggac tgctattaga aatcaatcac accataccac gcattagcga agtaataaca
     2221 ttcaagaacc tgcgttttac tattgaatca gccgacagac ggaaaataaa acgtgttaaa
     2281 ctacagatta tacaagaaca ggaatgaaaa agttggtata tatctttctt ttctgcttgc
     2341 tggcatcgtg cagcgaaact ccagcaccaa agccacgcgg attttttcgt atagatatgc
     2401 ctgagaagaa atacacttta tttacagata cctcttgtcc ctatcgtttt gaaactcctc
     2461 aatacagtta tgttttgccc gataccaatc gttttgcaga gccttgctgg aaatatattc
     2521 tatatccaaa attcggtgca caactttatc ttacctataa gcctttaaaa aataatcttg
     2581 cccaaatggc cgaagactcc aggggctttg cctacaaaca taccatcaaa gccaatggta
     2641 ttgacgaaaa cgttgttaaa acacaaaata atgtttatgg tgtggtatat gacatcggag
     2701 gtaatgctgc cagcaacatg caattttatg ttaccgacag cctaaaacat tttttaagag
     2761 gttcgttgta ttttaatacc attcccaatg cagattcgtt gcagccatcg ttggagttta
     2821 tccgcaaaga tgttttacac ctgataaaca ctttacaatg gagcaattaa aaaaatatcg
     2881 ctacacactt attttattga cagtagtgtt aatgttgtgt caatcttctt gcagactttt
     2941 caaaaaaaaa tgcgactgtc cgaaatggag cagtcagaca aaaaaatcac agacggttta
     3001 ataggtaatt ctatttctgc tttttatttt cagaacaaag aattttagct gcatatttaa
     3061 tcaaactgat tttaatcata ttcataacag cttaaattga agtttttatt ggattttacc
     3121 aatgaaagtc aatttttagg tcttggctaa gctttactat tgcagtcgaa ctttttatgt
     3181 tttaagtaaa ttcaaaggca attaataaac acatctaaca aaaaatatat tactatgcca
     3241 aaacattcat gggcagagcc ctttaaaata aaaatggttg agttgttgaa aatgacaaca
     3301 ccacaacaac gaaagaaagc attacaacaa gcaggttata atacctttct gcttaaatct
     3361 gaagatgtat atattgactt gttgacagat agtggtactt ctgctatgag cgatcagcaa
     3421 tgggcaggca tgatgttggg tgatgaagct tatgctggaa gcaaaaattt ttaccgatta
     3481 gaagaagtag taaaaaaata ttatggattt aaacatctta ttccaacaca tcagggtcgt
     3541 ggtgcagaaa atattctgtc gcaaataatg attaaaaaag gtgatgtggt tcccggcaac
     3601 atgtatttta ctacaacgcg tcttcaccag gaacttgcag gtgcaacgtt ctatgacgtt
     3661 attattgatg aggcgcatga cagctccaac ctgcatccgt ttaaaggaaa tattgacatc
     3721 aataaattag ataagttagt taaaaaattt ggtgccaata aaattcctta tgtctgtgta
     3781 gcaactacag ttaacatggc cggtggtcag ccaatttcta tggctaatat taaagagctt
     3841 agaaaatata ctgcaaagct tggcattaag gttatgttag acatgacacg cattgcagag
     3901 aatgcatatt tcatacaaca gcgtgaagaa ggttataaaa gaaaaagtat tgcaaccatt
     3961 gtaaaagaaa tatgtagtct gacagatgga gcaaccttta gcggcaagaa agatgctttg
     4021 gttaacatag gaggttttct ggcactgaac gatgataaaa agtttgaaga agcaagtaat
     4081 cttgttgtag tgtatgaagg actgcatacc tatggcggtt tagccggacg tgatatggaa
     4141 gcaatggcaa caggaattat tgaatctgtc aatgatgacc acatcagagc acgtattggt
     4201 caggtgcttt atttgggtga taagctaaat gaaatgaata ttccaattgt aaaccctgtg
     4261 ggtggacatg gaatttttat tgatgctaaa aagtttttac cgcatcttaa acaaacacag
     4321 tttcctgcac aggcattagc tgctgaaatt tacgaagaca gcggtgtgcg tactatggaa
     4381 agaggaattg tttctgcagg tagaaataaa aaaacaggcg accactatta tccggaatta
     4441 gagttggtgc gactgaccat tccacgcagg gtctatacac aggcacacat ggatgtaatt
     4501 tcagaatcaa tagaacgtgt ttataaaaac cgtgaaaaaa taaaaggatt agaaatgatt
     4561 tatgaaccta cctatctgcg attctttcag gcaaggttta aaaaaagata agtgtttttt
     4621 gtaattattg atttctaaag gaggcctatg cctccttttt tgttttgctg caaatgcaaa
     4681 aaacaggctg atttatgcat cttgtactgt cataaagcat tttgtacaat tattcttgga
     4741 ataagttttt tcagaagtat atttgaaaaa taagaataaa ccaactttta ataagcttaa
     4801 attataaccg ataattaata tagtcagaca gaaaataaac cgctcttttt ttaatgtctg
     4861 cccaaaacca aaaatatgtt aaatagaatt gtcggaatat ttacagcagc cctaacattt
     4921 accctgcttg taacctcttg tagtagccca cctgaagaaa aaaaagaagc gactactgtt
     4981 gctgcaaaaa ctgccgcaac agtagcgcca ttagaaaatg atcatttgtt aagtgtgaat
     5041 aatgctgtgt tgttaattaa gaattattta gacacaatta acaattccga aaatgccgaa
     5101 gctttaagaa aactacctgt tggcggatcc attagtgccg aagcattgaa aagaatgaca
     5161 gaagaaaacc ctgatgctaa cggctacatg ttttatggct ttaatgatgc agagcctgca
     5221 actttattta aagaagcaaa aatgcttgct gcatgtaagt tcaactttaa tccggatagt
     5281 atggtaagct atgaaactaa tgatgcaacc aatgtttacc tgataaacga taccgtaaaa
     5341 cataccttta actcattgac gtccgaagcc ataacaaact atctgtcgca gtacaacaag
     5401 gtaattcctt cagcagcttc tgtttatcac tattcaaata ttaaaggaaa tatgagtcaa
     5461 tacacctctt tctttaatga aaactttggt gatggcacag tacaatggcc ttatgccttg
     5521 ttttcatcag atgaattttt gacacgactt tatgaagcaa cagaaggtga aagtaatttt
     5581 ggtggcatac gttattattt tggattagac aaagaagcga aagaaaataa actcagaatt
     5641 attatttttg cagttaccaa atcgggtacc aatgtagttg tggataataa aattcttgag
     5701 cgacattggc ccccatcatc atgattgaat ggatcatttc tatactcgga tgcttgcaac
     5761 ctgcattgaa gtgctggtgg cattgttcgg agtaatgata tttaaaaaag ccggacttca
     5821 gttccggctt ttgattttct tgcttttgta tggtgcagca accgatattt ttggatggac
     5881 attttataaa ttaaaccgta cctatgtatt gattgctgtg gacatatata ctctggccga
     5941 agcacttttg atggttttca ttattcaatc aataggattg tttaacaatt taaagaatat
     6001 ttttaaacta atttacctat tactttttgt ttgctatgtt ggcgcatatt tcgttatgcc
     6061 attaataagt gatgatggtg cttatagagg tccctactcc tttggctact tgtcaaccgt
     6121 tgctgttttg gcagcttggg cttcgctcaa atggattgag ggtgcaggaa acaaactcaa
     6181 cagcccggtt tttgctttgc ttgcaggtgt atttatttat actttttgca gtgtgtttat
     6241 tgattccttt attggcacaa caacaggcga tgcagtgtgg tggattcatg atgcagctaa
     6301 cataactgct tatctgattt ttgcttattg cttttacctg attaaaaaat ccacaacagt
     6361 agaaaagtct gtaattaatt taccagaaaa agtttagtaa cacaggtatt attgcctttt
     6421 tcatcggtag caaaaaccaa ataaactcct gtttgtgcac gcttgccttc aaagttgttg
     6481 ccatcccatg tagcaattcc accttttgct gttgtttcat aaacaacgtt tcccgcaata
     6541 tcagttatct ttatattacc atttgccgta acaccacgta ctgcaatagg accatcgtaa
     6601 ttctctttca ccgggttggg aaatactttt aatcctgagc acgactctgt tccttgtgtg
     6661 gcatctccaa cataagaaat caatccttta tcggtgccaa taaaaacttc tccggtaacc
     6721 gggtcaatgg caattgaagt aatggcgttt gaaaataaag gactgtttga ggtattaaaa
     6781 ttctgaattt gctttgttcc atcagcagat aataaaaaca caccaccgcc attggtgcca
     6841 atccattttc tgttggctcc gtcaacagca atagctgtaa taatttcttt gtctaaaaga
     6901 tactgcgtaa taccgtctat cgttatcaga attttttgtg cttttgccgg tgtacttcct
     6961 gttgcattac cgggcgaata aattactgca ataccttctg atgttccaag ccaaatctga
     7021 ccttctttat cttcaaccat acaacgaaca tcatttacat ttaatccacc tgtttcctgt
     7081 gaaattcttg ctgaggcttg tgccacagga tcataaacaa tcaagccatt gctgattagc
     7141 gtggtccaca cctgaccata agagtcgcat aaaatatctc cgaaaaaaat atttacacca
     7201 gatggaataa cagactgtag attgatagac tgccaaacgc cctctttact caatacattc
     7261 aaaggtgtgt ttacccctga attcactacc caaagatttc ccgctgcatc ataagctaaa
     7321 ccaccaattt ttgtctgtcc cggatttcca aacgctcctt taaggctact gttgtcttta
     7381 ttgtacatct tcaccggaac attgttatca aactcaaaaa caccattgat gcgcgaacca
     7441 agaaaaaagt gtccgggatt tccgggctca ctgactacgg cttgtaaatc gagcatttga
     7501 ttgaggtcaa catttgaatt agctattgtt tttacattcc acgttttcca ttgcacacca
     7561 tcaaatgttg agtatccatc attaccataa ctgtcgctcc agcggttagt gccatgcgta
     7621 gtgaccaatg ttgtgccatt gtgaaataca caacttgcat ttggcgatgc agggctgttt
     7681 ggaactgtaa acgttatgtt gttgttgtcg gtataattta ccaatccatt gttgcgatca
     7741 gcaatataaa gttgattacc gctgcctgta gctacgccaa acatatctgt gttctgatag
     7801 ttagcattac cataaatctg acgcaatggc tgaaaattat tatcccattc attaacagaa
     7861 taattattca ctgcataaag caggtcattt tcagctttta attgtcgcag tgcaaagcct
     7921 ccgctaagca atgttgttcc tgtcgaaaaa tttgttgtgt taacgatgac tacactatct
     7981 gtagatgtag aatgaaaatt aacagccaga ttgccattaa gccagcacat cttattaaaa
     8041 gttactcccg taaaagtatt gggataaacc atttgccacg ttgcaaaatt attgagtgat
     8101 gggttactta tttctcctct gaggatgcca cctgcaatgg cagcataaat ataagttcca
     8161 tcagtagtaa ccgaataaac tggcacttca tttcctgtgg ttccaacaac gtaggtttct
     8221 tttatttcag cttttatcag atcgagcgct aaaacaccaa aacctgttgc aagatatgcg
     8281 aaccttccat taaaagctac atcattaata gtcttgtcac cggtagctgt tcttctttca
     8341 acatcagaaa tattaatgat gcccgtactt gatataagat caatgttaga atttacatag
     8401 cctacaaaaa ctacattttg tgtatggctg taatccatca gccttatgcc tacatcagaa
     8461 agcccgttaa tctttgaata gcgctgcaca ctgttgtctg acttgtcaat aaaatataca
     8521 gcctgatcgg tggcgcaata gacacgctgt ggtgtgttag acacaaattg tgcctgtgtg
     8581 tatgaaaggt gctcacgcca tgagcctaac ggcagattct gtgaataggt ctgtgttgca
     8641 gagagcaaca gaaaaaaggc aataaacaac tgtttgtaca tacggttcgg gatacggaat
     8701 atataactca acatgaggga atttatttta tggcctgcta tgagtttcta tttaaaatac
     8761 ttttgcacta caaaaatata aataatcgta atggcattga tagcggtaaa taccagaatg
     8821 ctgattaaag acaagttaga tggaacagga tggtttgcat tcgagaccct taagcgtatc
     8881 gtcacacagc atcccgaaca tcgttttatc tttttcttcg accgtaaatt caatgctgaa
     8941 tttatcttta gtagtaacat tttaccggtg gtcatttacc cacaagcccg tcatccgtgg
     9001 ctttggtaca cctggtttga aataagcatt caccgtgcac ttaaaaagta tagacctgat
     9061 ttattcttca gtccggaaag ttttatctgt agtaaggctg cagtgcctca ggtggcagtt
     9121 attcacgatt taaactttga acattttcca caatatatgc ctgcacaagt tgctcgttac
     9181 tacagtaaat ggtcgcgcat agcagcacag aatgcaaaga gaataggtac tgtgtcagag
     9241 ttttcaagac aggatattgt aaaaacctat aatataaaca ttgaaaaaac tgatgtgctg
     9301 tacaatggaa ttcatgatct gtataagcca ttatcagcag cagaacaaga gcaggtacgc
     9361 aatcaatata ccttaggttg ccgttatatt atttacacag gctctttgca tcagcggaaa
     9421 aacacggcaa acatggtgcg tgcttttgca cagtataaaa aaaagtatgg cggggaatta
     9481 aagtttgtac tggtctccga tcgcagtaaa atggaaggtg atttgcaatt ggcactttct
     9541 gaaataggca ttgaaaaaga tttaattttt acaggacgac tttctgccaa agcactggca
     9601 caactaactg catcggcaga ggcaatgatg tatgtttcgt tttttgaagg ttttggcatt
     9661 ccggtgattg aagccatgcg ctgcggagtt cctgtaatta cttccagtgt aagttgtctg
     9721 ccggagattt caggcaatgc cgctctctgt gttgacccac acagcgtaaa tgctattacc
     9781 ggtgctatgg agcaggtgtt gcaacatcag gaatttgccg aacaactaaa gcaaaaaggc
     9841 aaaatcagac aacaagattt ttcatgggat aaaacagctt cactaacgtg gcaaagtctg
     9901 cagcttgttc ttgctgaaaa aggcaaagag tgaaaaacca atcagtgtaa taagcgtaac
     9961 tcctgaaagc cacaaagcat aatgaatcgg tttgttttca tagcggtatt ctatccgatg
    10021 actgccttgt gctaccggta tggtcataaa taaataatct gtaacaacag gtgtaacttc
    10081 ttttccatta tcgtaaattt tccagccatg atagtgctgc tgcatcaatg ttaatgcaga
    10141 agatgtttca gcttttgctt caacaattat ttttcccgga ataaatgtct ttacagaaac
    10201 tgtattgcct gctgttggtt gaatattcaa caaatatttt tcgcttgcag gcacataaca
    10261 aacattatgc actgatgcag gtgccacaat gatgggttct tccaaataag caatcacagt
    10321 gtcagcaaaa taaatccagt tattggtaat ttgtctttgc ctgagtgatt catcatcaac
    10381 aaagcgtaca taatttttca gctgaaaaga gttatagcca tcccatgcag gttgtttttt
    10441 aaacagattt aagtttcgcc aaaaagggct tacccatcca ccggaatcgt tattgagtac
    10501 aacaggtcgc acttcaggaa caggaaaatc tgcaattgct ttttctttta cttcttttgc
    10561 tactacagaa gttttaaaat tgtaagtcac ggttacagga atatttaatt gtgcagccac
    10621 aactacattg gcagcagaca atgtaaatat tgtcatcagc agctttgccc gatctttaat
    10681 taataatgtc agcgacatca tcacaataaa aaacaactga ataaatcctt gaaaggctat
    10741 tgcctgagta aacgaaatat tgttgacagc gtcatgccaa ggtttaaagt tccagtcatc
    10801 ggtattcttg aaccaaaaaa atattgttac tactgcggca atgcaaccta aggtgactaa
    10861 tggcaactta aggcgactga atgcatcggg cgcagaaagt gcaatgccgg aaagcaacaa
    10921 tattcctgta ataaaaaaca atctatacac agagggaaat cggaataaat caagcatagg
    10981 aaaagttttg taaagccaac cgaaaaccgg ggtgtaactt ccaaaagatg caaagagcat
    11041 gacgatagtt gctgcaagaa taagccatgc agaacgtgat ttaggtctga tgataaaaag
    11101 cataaaccct gccaacggca acataccaat aaaaaccgat gacatgctga tgtctgtacc
    11161 aaaaaattca ctgtcgcggg ccgatgccag tggaaacata agagatatga gcgactgtgg
    11221 cgaaaaagga ccaaacgatg ctactttaaa tgaaagacct gttgctcgtg caagataatc
    11281 cgaactttga aaaacactat acaagatacc cgatgaacac aacaacaaca acacaacagc
    11341 aaaaatgctg tgcaataaaa aacgtttcat ccagtgctta ctctgacggt tgcggataaa
    11401 aagaaatacc gttacagtaa gcagaatata aaataaaatt attgcaaatg caggataacc
    11461 acccgacacc aacaagtaac caaacaatgc aaaatgcagt gcaaagcgcc agtgattatg
    11521 ttctgccata ttcttaaagg cgagaataac aaaaggcagc caacaggcag caatggtaaa
    11581 ggtaagatgc tgtgcattgc ctacaaacaa gccgcaaaac atataagcaa ttgcattaat
    11641 gagcgcaata aacgggttaa catgaaaacg ttgtaataaa aacaacatcc ccacaccacc
    11701 acctataaca tgcagcaaaa attccaattg cacactgtaa agtgtatatg gaccaaacaa
    11761 cgacatgagc cagacaacag gataccagac accactttgc gggtcggcat gcatggggta
    11821 gccaaactgc tcgtaaggat tccaaaaagg caaaatacca ttacgcagac actgcaccac
    11881 aaacatgcgc cacggataat actgatcagt aacatcccat tttaatgtgt tctgaaaaag
    11941 tgcaacctga tagtaaccaa caacagctat cagcaccaat aaaacataag gatatttctt
    12001 taaaaaattt atcacgggcg taaaaataga aggaattagt gatgttaaaa agataaattg
    12061 ataatagcag ctatttttcc gatttaaaaa attatgtttg tcatcaaact ttgtatatgt
    12121 ctgtcaaact tcttacagat tacctgcagc agcatacctt aataggaatt aaaagcggtc
    12181 ttcacagaaa gtcatttaca gacatttgga tggtaactgt tgaagggcgt ttcttttcgc
    12241 gcagttggaa caaaagtcat gacggatggt ttggcgaatt tttaaaaaac ggtgccggtc
    12301 aactgaaact taacaacatc atcctgaacg ttcatgtcca aaaaattgaa aaagacgatg
    12361 tcgttcaaca gaaaatagat ttggcttatc tttctaaata cacacaacct gaaaatgctt
    12421 cctatgcaca tggcatttcg catcccgatt atcatgactt tacattagaa ttttttccgg
    12481 aatgaataaa tatttggctt acagtttagc cagaatattc tctaatgcaa gttccttatc
    12541 taaaattgct ttcaattctt tgattccaat acgttgctgt tccatactat cgcggtgacg
    12601 aagtgtaacc gtattatcag ttaatgtctg atggtcaacc gtaatacaga aaggtgttcc
    12661 tatggcatcg tttctgcggt aacgctttcc aatagcatct ttttcatcat aatgacactg
    12721 ataagaaaat cttagcgtat tgaatatatt ctgagccacc tcaggcagtc cgtctttttt
    12781 cagcagcggt aaaactgcgc atttaatggg tgctaaaaat ggaggaatgt ttaatactgt
    12841 acgctgcgaa ccatcgttaa gtgtttcctc attgtaagct gtgctgagga ctgcaagaaa
    12901 aagtctgtcg agtccaatgc ttgtttccac cacataagga atatatgatg tgttttgctc
    12961 tgtatcgaag tagcgcaatt tttttccgga atatttttcg tgattagaca agtcaaaatc
    13021 tgttcgcgag tgaatacctt caagctcttt gaatccaaaa ggaaaacgaa attcaatatc
    13081 acaggcagca ttggcatagt gagccagctt cagatggtcg tgaaagcggt agcgttcagc
    13141 accaagacca agagaaagat gccagttaaa acgcttctct ttccagtggt tgtaccattc
    13201 catttctgtt cccggtttta cgaagtactg catttccatc tgctcaaatt cgcgcatgcg
    13261 gaaaataaac tgtcgtgcca caatttcatt tctgaaagct ttacctgttt gtgctatgcc
    13321 gaatggaatt ttcatcctgc ccgttttctg aacgttcaaa aaatttacaa aaattccctg
    13381 tgctgtttct ggccgcagat aaatggttgc tgcatcatct gtaacgctac ccaactgagt
    13441 agaaaacatc agattaaact gccgcacctc tgtccagttg cgcgaaccac tgacgggaca
    13501 aacaatttcc aagtcaataa ttatctgtcg cacttctgta agattgcctc cttctaaggc
    13561 ttctttaaaa cgtgcattta tcttgtccgc ttgttgacgg tattcaagca cccgtgcatt
    13621 ggtagaaaga aacatggcct catcaaaatt ttcgaatcgc ttggcagctt tttcaacttc
    13681 cttgcgtatt ttatcttcga ttttagcaac atgttcttct atcagtacat cggcacgata
    13741 gcgtttttta gaatctttat tgtcaatcaa cggatcatta aatgcatcaa cgtgtcccga
    13801 agctttccat gttgttggat gcataaaaat ggctgcatct attccaacaa tatcggggtt
    13861 tgtctgtacc atagctttcc accaatagtc gcgcagattt tttttcagct ccacaccata
    13921 ttggccataa tcataaacgg cactcagtcc gtcatagatt tcgctcgaag gaaaaataaa
    13981 cccatactcc ttggcatgtg atataatatt tttgaataaa tcgtctgttg tagccatagg
    14041 cggcaaaggt acgtggaata gattattgta aaactttatc ggataatttt aaagagtaat
    14101 gctgaatccg gaaaatcctg aaacgaaaag tcttaaatat ctatagcttc aagatatttt
    14161 tctgccaagg gcttgttcag atatcggata acataagcat tggctgaggc gcggttaata
    14221 tcgtccggac tgatggatga tgacaacaca atgatgcggc atgattgacg gatattttct
    14281 gcaagtgtgt taaactcatc taaaaaagca aaaccgtctt ttacaggcat attcaggtca
    14341 aggaaaatta cctgaggcaa tggtacctta ctatctttgg caattgtgtt cagatactca
    14401 atggcttcat cggcagaggt ttttacagta cacacttcgc tgaaccctga agaggtaata
    14461 atacgctcgt tgatgaaatt atctatttca ttatcatcaa tcaacagaac gtgttgcaat
    14521 cgagaaggag aagatgattt cattgaatct tttcctgaaa atgttagtgg tgacaggtgg
    14581 aatcgaacca ccgacacaag gattttcagt cctttgctct accaactgag ctatgtcacc
    14641 gttaataagt gcgcaaaagt aaaaaaataa tgaatagaaa aaatttttgt tttttttagt
    14701 cacctccatt tcaaagttat ttgaattaaa attttgtagt attgttaact ttgaacttaa
    14761 ttttcctaaa ggaagagtcc gataaaactt actttgtttt tagaatattt ttgtaactga
    14821 tttttaccca tacaaagaaa accatgcatg attttattaa taaagagcga ctttttgcct
    14881 tgttgacttc attaattttt atactattgg cattacccgg attttgcgtt tcgtctgagc
    14941 ctgaacatga gcatcatcct cacaaagcgg aaataggcat tggcaattct gccgtttact
    15001 ttactaagga caaaacttta aattatggct ttcattttca tttcagccgc aacatcaaac
    15061 attcaaactt ttccgttggt gcagaatacg aaagaatttt tgaccgccat ggtcataata
    15121 tgattggtct tgtcgcagga tatagtccgg cacatcgtgt tgaactcagc attacacccg
    15181 gaattttatt tgaagacgct gagcccaacc atgccgatgt ttctctccat tttgaagcag
    15241 gttatcattt tgatattaaa aacattcacc ttgggccaat agttgaatgt gcagttgaca
    15301 ggcatgatgt tcatttcagc attggactgc acttgggtat tggaattgga aaattgattg
    15361 aataaataag cagtaaacac atggcaggac taaatttaac tttgtaaatc aataaaagaa
    15421 taaacaatag aaactgtaac ctgatttaag gaagagatct atttatctct gattatgccc
    15481 ttcaggatga taaaaaaata ttgcagttgt cagtttttac catgacaaaa taacgaacaa
    15541 gatttaatgg aaatagttaa aagccatttc aaacagggta tattttattc ttgtcagtga
    15601 taataatgaa aacaatgtta taaaaaagtt tgttaaactt tagtctattt ttacatttaa
    15661 aataattatt aaaatgaaag aacgattcaa cctatcaaaa gccgacccta aagcatttga
    15721 tgctatgctt aatcttgaaa aatatcttgc agaaagtgga ttagataaaa ttctttttga
    15781 gttgataaaa acacgtgcat cacaaattaa tggttgtgcc tattgtatta acatgcatgt
    15841 gcgtgatgcc atgaaaattg gtgaaacatc tcaacgatta ttcttattgg atgcctggcg
    15901 cgagactact ttgtttacag aaaaagaacg agctgttctg gcacttacag aagccatgac
    15961 attgattgca aataatcctg ttagcgatgc ggtttttgat gacgccaaaa atcatttaac
    16021 caacaaagaa atggcagcag taatgatgac tattgtaacc attaatgcat ggaacagaat
    16081 tgcaataacg tcaagaactg ctttggacta agtaatttta acccacaatt gatacctgat
    16141 taaaacaatt ttagtatttc ctatttcaga tggactccca atctattatc gaagaattaa
    16201 agaaacttaa taaaatcagc agtcaacttg atatttctca agaagaaaga gcatcgctgc
    16261 taaatcagac tgcagaatat actaataatt ttatttcaac cctgcatacc ataaaaggtt
    16321 atcaggaaaa aatagttggt aatttgtcca tcaataacaa acctgcttct ttgaaggatt
    16381 tgctaactct ttatcagcat gaagttgcag aaagtggcat taatgctgcc tcgccaaaac
    16441 atttaggcta cattccgggt ggaggcgttt acactgctgc cttggccgat tttattgctg
    16501 caattaccaa tccgtttgcc ggagcatatt ttgcggctcc cggtgctgca actatagaaa
    16561 acgaagtagt tgattggttg aaaaaagttt tttcttttcc tgaaagtgct gttggatgtt
    16621 tatcatctgg tggctctgtt tctactttaa ttgcatttac tgctgcaaga gataaattta
    16681 aaattaaaaa cgaagtcatt cccaaaagtg tagtttacct tagtgagcag gttcatcatt
    16741 ctacccaaaa agctttacgg ataattggtc ttgaagatgt cattgtgcgt tatattcctt
    16801 tggatgattg gcataagatt aaaccggatg cactaaatgc tttagttgaa aaagataaag
    16861 cagaaggact aaatccattt ttaattgttg ccacagcagg cacaaccgat accggtacta
    16921 ttgatccatt aaatgatatt gccactatag ctgctcagca aaaactttgg tttcatgtag
    16981 atgctgctta tggaggattt tttattctaa catcaaagaa aaatttattc aaaggtattg
    17041 aacgtgccga ttcaataatt gttgatccgc ataaaggttt gtttttaccc tatggtgttg
    17101 gtgcagtttt agtgaaagat agtaatgctg ttttacactc acattattac actgctaact
    17161 acatgcagga cggacagaat gaagagttgc ttaaaagtcc ggcaaacctt tcaccggaac
    17221 ttaccagaca ttttcgtggg ctccgtgtgt ggttaccatt aaaatatcac ggcatagaac
    17281 cttttattgc ctgccttgaa gagaaacttt tgttagtaca atatttcaga atgaaattgg
    17341 cagcgcgggg attttgtctc ggcccggaac ctgatttatc tgtaagttat ttttggtatc
    17401 cgtttgcaaa tgacgctgat gaaaagaaca agctgctgat ggaagaaatt cataaagatg
    17461 gttctgtatt cttaagttcg tctattatta ataatcgttt tgtaatcaga attgcaatct
    17521 tagcgttcag aacaaaaaag gaaaccattg acgaagctgt tgaaatgatt gaccgctgtt
    17581 tagaaaatat attatcaaac tattcgtgaa aaacgtcttt gctaaaaaaa attttctgtc
    17641 cttcatccac cataaatctg ctttttttta attttttgta accttttgtt ttttcattta
    17701 ctctaattga gtataaacat taaaaaataa taaaaaatga aaaaaacatc attacaacaa
    17761 gacaactcac ttgaaactat gaagtcttca agtaagaaaa aaacaaggtt taatttaaaa
    17821 acacactctg tatttctact cctgctttgc cttggcactt tagttacgtc ttgtaaaaaa
    17881 gataacgact ccaatccttt agaaagcact caatattaca gccttcaaga tggtatgcgc
    17941 aaactttgga acgaccatat gcaatatacc tacgcaaccg ttgatgctta cttcaacaat
    18001 ccttccgggc tatcttcctc tttgaatcgc ttattagaaa atcaaaaaga tattggtgct
    18061 gcaattgttc catactatgg tcaggctgcc ggtgacacac tttcggcttt gctgacaaca
    18121 catataaact tagctgttcc tgttcttgaa gccgcaaaaa acaacgatca gccagccctt
    18181 aaccaggcat tagcaaactg gaaaaccaat gctaatgata ttgccaactt tctgtcaacc
    18241 gccaatccaa aaaactggcc tcctcatgat atgcaacaca tgatggaaat gcatattgac
    18301 caaacaacaa cctatgccgt tgatttgttg aacaaggatt acaacaatgc aatcactcat
    18361 tatgatgagg ctaatcaaca catgcttgat atggctgatg accttgctaa aggaatagca
    18421 ttacaatttc ccgataaatt ttaaatcaca ctcaactttt aaaaattaca tcaatggaaa
    18481 ataaaatctt atcccagact tttaatctgc ttaaatacac ttttgttgtt gttcccattg
    18541 ttgccggtgc agataaattt acaaacctgc ttaccaattg gccacaatat atcaatccaa
    18601 caatagccgg aatgcttcct ctaacacctg cagtgtttat gatgattgtc ggtgtaattg
    18661 aaatcgcagc aggtattatt gtattgaaaa aacctgctat tggtgggcaa atagtagcag
    18721 catggttaac acttattgca ctgaccttgc tcttagggtt caattatata gatgttgctg
    18781 taagagatct agtaatggca atatcggctt tctcaatggc tcaattagct aaatttgtaa
    18841 aacaatagta tggaacttta taaagaacta tccgaagcac aaattgcaga acgtatatta
    18901 aaaggagaaa aatcgcttta cgaaattatt gtaaggcgat ttaatcc
//
LOCUS       DAOQNP010000064        18633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1044_length_18633_cov_9.822263, whole genome shotgun sequence.
ACCESSION   DAOQNP010000064 DAOQNP010000000
VERSION     DAOQNP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 18633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18633
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_18633_cov_9.822263"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKY09_09240"
     CDS             complement(<1..659)
                     /locus_tag="PKY09_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HNB13081.1"
                     /translation="MKIFRFGWIYFIAAFLFMAVGNTTLAQGNKKELEKKRERLQREI
                     NETNKLLKLTEKNKNATQAQLDALRKKISLRNELINSINVEIKSLNGEIDKTGKSIDS
                     LERQMKQLREDYAAMIQFAQRNRNKYQTMMFVFAADDFNQAYKRLKFLKQFTENRRNQ
                     VEQIAEVQTSLNSKKADLQQKKNEKTGLKQTQECQRKNLESEKQEQDKINCVPGYYDV
                     L"
     gene            complement(736..1572)
                     /locus_tag="PKY09_09245"
     CDS             complement(736..1572)
                     /locus_tag="PKY09_09245"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNB13082.1"
                     /translation="MNKILQITLCVLIPMLVFNACKVTKKSEKPIVIKPIAKLEEQGA
                     ETLLKNIVDSAFFGKWISGKATVNTNIDGDKNNFNVSLRICRDSAIWISVSPLLGIEA
                     VRILLTPDTVRFLDRINKEYKVSGYKEINDLLYAGNLDFDIIQGILTGSLFVYKRNKF
                     HSVYLEDQYYILSTLSKRKLKRSLEEKDPSKPVIQDFYVDGNNYRIMKLAIEDNRIQK
                     SLETNYSGYSYTAAGYFPHHSITNIRAEKNILVDLEYTKVSVEDTLEFPFTIPKSFKK
                     AR"
     gene            complement(1565..>2164)
                     /locus_tag="PKY09_09250"
     CDS             complement(1565..>2164)
                     /locus_tag="PKY09_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13083.1"
                     /translation="EAIGLFPNESSLYLLNGIAKSQLNLPAEAINILNQGVKLVVDNN
                     QQLIQFYSNLGDCYNKTKEYSASDSSFDKALEIDPNEPFVLNNYAYYLSVRKEKLEQA
                     EKMSKQSNELQPNQSSFLDTYGWILYQEGKYDEAKKWIEKAMENGGENSGTILEHYGD
                     ILFKLNQSDKAFEYWKKAKQSGDASGLLDQKIRDKKLYE"
     assembly_gap    2165..2264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2265..3160)
                     /locus_tag="PKY09_09255"
     CDS             complement(<2265..3160)
                     /locus_tag="PKY09_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13084.1"
                     /translation="MRSFLAVRKVWVFLFMIGVFATSCKTSKQTTSANKETNLSEENN
                     TKLMYFFVNANKEKILGNEGRAAELFAECLKIDPRNDASLYEMAQLYANRKKYAEALN
                     FAKNAANINPKNEWYKQLLAEIYQRTNQWNEAALIYQNLTVEYPQRIDFYYQWAYALV
                     NASKLNDALKAFDKIESIIGPDKEIALRKEQIYLKQNKVDKAAEVLENYISNNPESMD
                     MYSLLYELYSANNMPDKALSVIDRMKAVNPNEPRIYLNLADYYRNKGDKEKSFENLQK
                     AFTNRDLDTDIKVKIISSYIPLL"
     gene            complement(3173..4174)
                     /locus_tag="PKY09_09260"
     CDS             complement(3173..4174)
                     /locus_tag="PKY09_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB13085.1"
                     /translation="MKIIIPMAGMGKRMRPHTLTVPKPLIPVAGKPIVQHLMEDIISV
                     CPEKVDEIAFVVGHFGSQVEKTLTDIAASLGAKGTIHYQDEPLGTAHAILCAQSALKG
                     KVVVAFADTLFKSNAKIDAEHDGTIWVKQIEDPRQFGVVKVNAENIITDFVEKPVQFV
                     SDLAIIGIYYFKDGENLKKELQYLIDNNIKDKGEFQLTNAMENMKNKGLKLVPGKVEE
                     WLDCGNKDATAYTNGRMLDIKYPENYISPSAKITDSVINKPCFIADNAEIIGSVIGPG
                     VSVGAKTKIENSVISQCIIQTNSNISDAVITNSMIGNYCVYKGKSDDLSMGDYSTKA"
     gene            complement(4254..5618)
                     /locus_tag="PKY09_09265"
     CDS             complement(4254..5618)
                     /locus_tag="PKY09_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNB13086.1"
                     /translation="MKTTTITRIFVVEDDDWYREFLHYVLSLNPDHAVTAFSNGKEVL
                     KNLHLHPDIITIDYHLPDTNGSTLLGLIKEQCPDADTLIISEQHSIDTVVELLKSGAV
                     DYFVKSKDIRDKLLNTVELIKERQGLKQKISRLEKEVEKRYNFQDRLIGSSNNFQSLF
                     PLIEKAAATNITVTISGETGTGKEEIAKAIHYNSSKEKSRFVAINMAAIPIDLAESEL
                     FGHEKGAFTGALQSRIGKFEEADGGTLFLDEIAEMELSLQAKLLRVLQEKEVVRVGSN
                     KPIKINTRIIAATHKNLLDEVKKNRFREDLYYRLYGLQINLPPLRERDKDIILLAKYF
                     ITQFCKDNQLQVKKISAKAQNKLLAYQFPGNVRELKSITELAVVMSNTDMIEPDDILF
                     SQSNFEAELLSQNLTMEQYEKKIIMHVLSQCNGNVVQAAEKLNIGKSTIYRLLNKERS
                     NSED"
     gene            complement(5845..6867)
                     /locus_tag="PKY09_09270"
     CDS             complement(5845..6867)
                     /locus_tag="PKY09_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNB13087.1"
                     /translation="MNRNLKDISNLLSKLTVRRLFNFSKILCSYYLSRITGKANVSGM
                     PVSISIEPTTSCNLRCPECPSGLRAFSRPTGMLQPDFFEKVIDELSPDLLYLILYFQG
                     EPYLNKNLFEMIRYASQKKIYTATSTNAHYLDDDNARKTIESGLDRLIISIDGTTQET
                     YQQYRIGGSLEKVIKGAENMVKWKNKLKSKTPHLIFQFLVVKPNEHQIKEVQQLAKSI
                     GIDEVVYKTAQLYDYVHGNELMPNDLSHSRYKKNKDGTYSIRNPLLNHCWRMWHSCVI
                     TWDGNVVPCCFDKDASHRLGQVATEGFKKVWHSEPYNRFRKLVLKSRSNIDICTNCTE
                     GTKVWT"
     gene            complement(6996..7667)
                     /locus_tag="PKY09_09275"
     CDS             complement(6996..7667)
                     /locus_tag="PKY09_09275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNB13088.1"
                     /translation="MQPSILFEDYYLAVLHKPAGLMVEDDNFGNPSLQNWFLNHLQAK
                     FPTNKNYYIGFPHRIDRVTEGLIIVAKTKSALQNLNEQFNNKSITKIYHALTEHSLTA
                     AEGTLTDYHQKNTKLKKAILSAKKKEGFVRAELRYKEVCNTKSNAFLYKIELITGRYH
                     QIRAQFSSRNAVVVGDSFYGAVTDYQQGSIALIASEILFSHPKTNQAMHFTLPLPQNL
                     KWQCR"
     gene            complement(7680..>9943)
                     /locus_tag="PKY09_09280"
     CDS             complement(7680..>9943)
                     /locus_tag="PKY09_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13089.1"
                     /translation="WSTGATTSSINVSTAGTFTVTVTNANGCTGSTSATTTVISNPTP
                     SISGTTTFCSGSSTILNAGSGYSTYLWSTGATTSSINVSTAGTFTVTVTNANGCTGST
                     SATTTVNSLPVAAITPSGNVTTCNSSQLLNAATGVGFSYQWRLNSNNISGATADNYTA
                     SISGNYDVVVTLSGCSATSSPTVLTLGTGSPQITANGSTNICAGANVALSAPVGASSY
                     QWYRNNVALGTAVNQNYYASSAGQYYCKVTGACSGNSNVIVISVNNNPAPTISSSTAL
                     SFCAPGYVTLTANTFTGVNYQWQFNSVDIPGATSQSYNATQAGGYRVIQTANGCSKSK
                     TVSVKNATSVTAQIHTNDQTTLCSPTGTVNMELVNPIPGYSYQWQKNGTDIINETSST
                     YAATSSGSYTCKVSASCGTTISNAIPVSIGSFSASVLPAGTVTICTGASVVLSASTGT
                     GFTYQWKNNGVNINGETSPTLTVNTAGSYSVYMTSPCGNATSNVVTVNSATVSAVISP
                     SGTATICAGQVFHLSVTSNPVYTYQWYRNNVALNGAVNSSCNVTSAGTYYAIVSLNGI
                     CPVTTSNLVLTVINNPTPTITASGPTTICTGQDVVLSTNSWPGVVYQWTKNSIDIVGE
                     TGTSYSANTTGGYRVRQTGNGCSKLSPLVQVKVNPCRIAGEPDVFETEYALTVFPNPV
                     LQDATITISSDLTVDNGNLLIYDVLGNCVQRIEHLQSNSLDFNKENLKPGIYFVYFTN
                     GDGFKLTSKFIIQ"
     assembly_gap    9944..10043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10044..12517)
                     /locus_tag="PKY09_09285"
     CDS             complement(<10044..12517)
                     /locus_tag="PKY09_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNB13090.1"
                     /translation="MKGIFKTMMTALFIIQATYFLNAQDSKIFWREVSTIPVSGERLT
                     VPSSYRALNLDLNLLQQYLNTAKAEPSQTANLKNGLMLELPMPDGSFERFSIFQYNVM
                     HPDLAAKFPQIKTYTGQGIDDVTATIKLDVTQFGFHAMIRSSKGDVYIDPYNQNTVNY
                     YMSYERKNLIRQNSFECLLADETAMEIQNANGNTVQRTNGTQLRTYRLALACTGEYAA
                     FYGGTVSGAMAGMVTTMNRVNGVYESELSIRMVMVANNNLIVYTNSSSDPYTNNNGST
                     MLSQNQTTCDNIIGSSNYDIGHVFSTGGGGVAYLGCVCSSSNKAKGVTGNGAPSGDGF
                     DIDYVAHEMGHQFGGDHTFNSSTGSCSGNRSSTAAYEPGSGITIQAYAGICGSDDLAP
                     HSIAYFHGYSLDQMITFSNTGGGNSCPVTTSTGNTAPVVTNMGYNCSIPISTPFVLTG
                     AATDANGDALTYSWEERDLGPAGAWNVQSTTAPMFRPFPPTTSPSRTFPQMSDVVNNT
                     TTVGELLPNQARTLKFRLTARDSRTGGGGIMHPDSNLIVTVVNAGGAFAVTAPNTAVT
                     WAGNSTQTVTWNVSGTTGSGINTANVKISLSTDGGYTYPTVLLSSTPNDGSQTITVPN
                     ISTTTARIKVEAVGNIFFDISNTNFTITTSTGLTTITTSPVSPLSFCKGASLNVSYTV
                     DAAANAGNVFTAQLSNSSGSFASPVNIGTLTATTAGTITCQIPLGTATGAGYRVRVIS
                     SNPSVIGSNNNSNITVNSNPTPSISGATTFCSGSSTTLNAGSGYSTYLWSTGATTSSI
                     NVSTAGAFTVTVTNANGCTGSTSATTT"
     gene            complement(12690..13496)
                     /locus_tag="PKY09_09290"
     CDS             complement(12690..13496)
                     /locus_tag="PKY09_09290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB13091.1"
                     /translation="MQYDPIKRSLGEVFNKSPFLRKTFYRLLDLLLLRAWHIHKELKQ
                     WESTVGDNAHILDAGSGFGQYTYWLASRNRNWKIYAVDVKEEQVSDCNTFFQKAGYKN
                     VQFGIEDLTKYQSPSTYNLVVCVDVMEHILEDEQVFKNYAASLKAGGMLVISTPSDQG
                     GSDVHGDDQQSFIEEHVRDGYNIDDIKVKLLRNGFSRVEAKYSYGTPGKISWRLSMKY
                     PISALGYTKAFFIILPFYYLLFYPICFVLNYLDVNIKHPTGTGLIVKAWK"
     gene            complement(13505..13870)
                     /gene="rbfA"
                     /locus_tag="PKY09_09295"
     CDS             complement(13505..13870)
                     /gene="rbfA"
                     /locus_tag="PKY09_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNB13092.1"
                     /translation="MDSTRQQKISRMLQKEISSLFQLEGSSFYGNAFVTVTNVRVTPD
                     LGIAYVRLSLFKQKNAEEIVSNLNHKMHDIRRKLGNRIRNQIRHIPELKFFNDESLDY
                     AEHIEKLFKEIHKNDPKEG"
     gene            complement(13893..14561)
                     /locus_tag="PKY09_09300"
     CDS             complement(13893..14561)
                     /locus_tag="PKY09_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13093.1"
                     /translation="MKKILAAALFFATTITSAQVITVAEQTSTFSTGQQPAIVTTCFN
                     NNLKDVTNSWTTYMKSLKSKKVTAGKEETFTDNVLIKDWGNNPVDIYARFEENKSDNS
                     VKVMVAFDLGGAYLSSTVDATKYGVAEQMVKNFAIETTKAPIQSQLKDAEKLLGKMES
                     DRGSVEKDIKTLRSDIEKYKDNIKKAEDDVYKKENDLSKKKSEIDNQRTAIQLLTNKL
                     GEIK"
     gene            complement(14790..15635)
                     /locus_tag="PKY09_09305"
     CDS             complement(14790..15635)
                     /locus_tag="PKY09_09305"
                     /inference="COORDINATES: protein motif:HMM:NF039394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HNB13094.1"
                     /translation="MRLIVILLLLFAQSVVAQPLIDIASLNYSYHRPLKFTNNESATL
                     QYTNAFLTLPIRSGNNIFIINPSFDRFDFKFRQQSKLYMNGQIALTWQHQWKDKRFKT
                     ALVVIPKSISESKYWLKKDSYQIGGAILQTYQRSEQLSYKLGLYYNSEFFGPYFLPLL
                     GIDYRITPRWNLWGILPSSLNGEYKAIINKLHFIISLKTMTLSYRKDDSYYLRTNDNL
                     IRIAADFYVNKQNVLFLETGHSVFRKFVLGTKNHNIDTQSTLSVQNSWMVRVGYAYRV
                     RLDKL"
     gene            15734..16357
                     /locus_tag="PKY09_09310"
     CDS             15734..16357
                     /locus_tag="PKY09_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNB13095.1"
                     /translation="MFNILKKLLILLSLPLATNAQSFKASLIGGINASQVSGDELGGF
                     NKAGVMFGGSAILPVTPKSDVAMELLFIQKGSKTPTSKDNNNADYYKMSLNYLEVQIN
                     YTYHASKKIGMHVGPTFGVLIGEKEEDIAGELTERPEFQKTEIGIAGGLSFHFSEKVG
                     LYMRLSNSLLPIRKMGADTRYFKSGQYNSGLAFFLTYTFSSKDKQKQ"
     gene            16357..16917
                     /locus_tag="PKY09_09315"
     CDS             16357..16917
                     /locus_tag="PKY09_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNB13096.1"
                     /translation="MKNKIVVAITGASGSIYASILLGKLAQLKEQFSDVAIVFSKNAT
                     DVWQHELGNDTYKSLSFKRYDTMDFNAPFASGSAQYNTMIIIPCSMGTMGRIAAGISN
                     DLITRAADVILKEHRKLILVTRDMPLNLIHINNMKTITEAGGIICPACPSYYSKPQTI
                     NDVAATVVDRALDLAGFSLNSFRWNE"
     gene            16910..17884
                     /locus_tag="PKY09_09320"
     CDS             16910..17884
                     /locus_tag="PKY09_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13097.1"
                     /translation="MNKQSSAFEMISNLLFIRLQQLKRELISTGWFYSLIILIITGLL
                     GFLLYHAVQKQIHSFYLAALIIVAVYTIHNSRNDLQFIRQQLRNPFFLLTTEYILLAL
                     PVIFLLLLKGFFAAGCTIITGCVLVALIAPKNSVYLLFPKLASWIPSEMFEWMSGIRK
                     TKGVVAVFIIVMYATSFLKFIPLIFLWLITITIAGFYQFGEPLTLLYLHENAKRLLSN
                     KIKSALSLFVKISIIPVLVNIFLHPDIWWVDLMVYTLCLLVITCAILIKYAVYRPNER
                     LTAGSILLSVVCISPTLPFTIPIPVVVAAMKYKSAIQNLENYFHDQNH"
     gene            17868..18548
                     /locus_tag="PKY09_09325"
     CDS             17868..18548
                     /locus_tag="PKY09_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB13098.1"
                     /translation="MIRIINLKISFDKHQVLDIEKYNFTTGKVHGIIGLNGAGKTTFF
                     NAVCKFLKRDAQAVLINNKPFERHDAAYLETTNYFYENITAQEYLSLFPSDNLLFNKN
                     NLIELLQLKSCGLIETFSTGMRKKLALIAIICQEKPVYILDEPFNGLDLESNKILEML
                     IQKLAESGKTIFLSSHILSPLTTSCTEIHLLRNGNFEKRFQPEQFNEIENELFSNLTE
                     KVNSILKK"
BASE COUNT         5411 a         3438 c         3364 g         6220 t
ORIGIN      
        1 tttaatacat cgtaatatcc cgggacgcaa tttattttat cttgctcctg cttttcactt
       61 tcaagatttt ttctctgaca ctcctgcgtt tgtttaagtc ctgtcttttc atttttcttt
      121 tgctgcaggt cagccttttt actgttcagg cttgtctgta cttctgcaat ctgttcaacc
      181 tgattacgtc tgttttcggt aaactgcttt aaaaatttta gccgcttgta agcctggtta
      241 aagtcgtcag cagcaaaaac aaacatcatg gtctgatatt tatttctgtt gcgctgtgca
      301 aactgaatca ttgctgcata atcttcgcgc aattgtttca tttgtctttc taaggaatca
      361 attgattttc cggttttatc aatctcaccg ttgagcgatt taatttcaac gtttattgag
      421 ttgatgagtt cattacggag tgatattttt tttctcaagg catcaagttg tgcctgagtg
      481 gcatttttgt ttttctcagt aagtttcagt aacttgttgg tttcattgat ttctcgctga
      541 aggcgttctc ttttcttctc tagttctttc ttattacctt gtgccaaggt agtattgcca
      601 acagccataa ataagaatgc agcaataaaa taaatccacc caaaacggaa aattttcatt
      661 gcagcaaaat taggtttagt gctcccctta gtcctgatga aagaaattaa ttattgacag
      721 atttatgcac gacaatcagc gcgctttctt aaagcttttg ggtatggtga aaggaaactc
      781 caatgtgtct tcaacagata ccttggtata ttctaagtca acaagaatat ttttttctgc
      841 tctaatatta gttatagaat gatgaggaaa atatccggca gcggtatatg aataaccgga
      901 ataatttgtc tcaagtgatt tttgtattct gttgtcttca atggctaact tcataattct
      961 atagttgttt ccatcaacat aaaaatcctg aatgacaggt ttacttggat ccttctcttc
     1021 aagcgatcgt tttagtttac gtttgctgag cgtactgagg atgtaatact gatcttcaag
     1081 ataaacagag tgaaatttat ttcttttata aacaaaaaga cttcctgtca gaataccctg
     1141 aataatgtca aagtcgaggt tgcctgcata aagtaaatcg ttaatttcct tgtagcctga
     1201 aactttatac tctttattga ttctgtcgag aaaacgaacc gtatcaggtg tcagcaatat
     1261 acgaacggct tctataccaa gcaacggtga aacagaaatc cagatagcac tgtcgcggca
     1321 tatacgcagt gaaacattaa agttattttt atcgccatca atgtttgtat ttaccgttgc
     1381 tttaccacta atccatttac caaaaaaagc actgtcaaca atatttttca ataaggtttc
     1441 agctccttgc tcttcaagtt ttgctattgg cttaataaca attggctttt ctgatttctt
     1501 ggtaacctta caggcattaa ataccagcat gggtatcaac acacataaag ttatctgcag
     1561 aattttattc atacagcttc ttatccctga ttttctggtc taaaagtccg gatgcatctc
     1621 ctgattgctt tgcctttttc cagtattcaa atgctttatc ggattgattc agcttaaata
     1681 aaatgtcacc gtaatgttcc agaattgtgc cgctgttttc accgccattt tccatagctt
     1741 tttcaatcca cttttttgct tcatcatatt ttccttcctg atacaatatc catccgtagg
     1801 tatctaaaaa agatgactga ttgggttgaa gttcattact ttgttttgac atcttctcag
     1861 cctgttctaa tttttctttt ctaacagaaa gataataggc ataattattc agcacaaaag
     1921 gttcattggg atcaatttca agtgccttat cgaatgagct gtcagaggct gaatattctt
     1981 ttgttttgtt ataacaatca ccaaggttgg aataaaattg tatcaattgc tgattgttgt
     2041 caactaccaa cttaacacct tggtttaaaa tattaattgc ctcggcaggc aagttaagct
     2101 gactttttgc aataccgttt aacagataaa gtgaagattc attaggaaac aatcctattg
     2161 cctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcaat ggaatgtatg
     2281 aggaaattat tttaacttta atatctgtat ccaagtcgcg atttgtaaaa gctttttgta
     2341 aattttcaaa tgatttttct ttatcaccct tgttgcgata gtaatcggcc agattgagat
     2401 aaatgcgagg ttcattcgga ttaacagcct tcattctatc tattacagac aaggctttgt
     2461 caggcatgtt gtttgctgaa tagagttcgt acaggaggga gtacatatcc atgctctccg
     2521 gattgttgct gatataattt tcaagcactt cagcagcttt gtcaaccttg ttttgtttca
     2581 gataaatttg ctctttacgt aaagcaattt ctttatcggg gccaataata gattcgattt
     2641 tatcaaaggc tttcagggca tcattcaact ttgatgcatt taccaatgca taagcccact
     2701 gataataaaa gtctattcgt tgcggatact caaccgtaag attctgataa attaatgccg
     2761 cttcattcca ttgatttgtt ctttgataaa tttctgccag cagttgttta taccattcat
     2821 tctttggatt aatgtttgct gcatttttag caaaatttaa tgcttctgca tattttttac
     2881 gattggcata gagttgcgcc atttcataaa gcgaggcatc gtttctcgga tcaatcttta
     2941 aacactctgc aaaaagttca gctgcacgac cttcattacc caatattttt tctttgttgg
     3001 cattcacaaa gaagtacatg agttttgtgt tattctcttc ggagagattg gtttccttat
     3061 tggcagaagt tgtttgttta cttgtcttac atgatgtggc aaacacacca atcataaaca
     3121 gaaaaaccca aactttacgt actgctaaaa aactcctcat tttatttttt tattatgctt
     3181 ttgtcgaata atctcccata ctcaaatcat cagatttacc tttgtaaaca caataattgc
     3241 ctatcattga attggtgata acagcatcgc tgatgttgct gttggtttga ataatacatt
     3301 gtgaaattac actattttca atttttgttt ttgcaccaac actcactccg gggccaatca
     3361 cacttccgat aatttctgca ttatctgcaa taaaacatgg tttgttgatt acactatctg
     3421 taatttttgc tgatggcgaa atataatttt ccggatattt aatgtctaac attctaccat
     3481 tagtatatgc agtagcatct ttatttccac agtcaagcca ctcctctact ttaccgggaa
     3541 ccaatttcag tcctttgttt ttcatgttct ccatggcatt ggttaactga aactctcctt
     3601 tgtctttaat attattatca atcaaatact gaagttcttt cttcagattt tcgccatctt
     3661 taaaataata aatgccaatg attgccagat cagaaacaaa ctgtacaggt ttctcaacaa
     3721 agtccgtaat gatattttct gcattcactt taactactcc aaactgacgt gggtcttcaa
     3781 tctgtttcac ccaaatggtt ccatcatgtt cagcatctat tttagcatta cttttaaaaa
     3841 gtgtgtcagc aaatgcaaca actacttttc ctttaagcgc agactgcgca cataaaatgg
     3901 catgtgctgt tccaagtggt tcgtcctgat agtgtattgt tcccttggca cctaaagatg
     3961 ccgcaatatc tgtcagtgtt ttttctacct gactaccaaa atgacctact acaaaggcaa
     4021 tttcgtcaac cttttcaggg caaacagaaa taatgtcttc catgagatgt tgaacaatag
     4081 gtttacctgc aacgggaatg agtggtttag gtactgttaa agtgtgaggg cgcatgcgtt
     4141 tgcccatgcc ggccattggg ataattattt tcatacgtaa tttatcgtca ttatttggag
     4201 cggcaaaggt aatgaagtga tggctctgtt caaactcaaa tacaaaacat acgctaatct
     4261 tcagaattgc ttctttcttt atttaacagt ctgtaaatgg tacttttacc gatgttaagt
     4321 ttctctgcag cctgaacaac attaccgttg cattgcgaca acacatgcat aatgattttc
     4381 ttctcatact gttccattgt caggttttgt gaaagcagct cggcttcaaa attactctgc
     4441 gaaaacaaga tatcatcagg ttcaatcata tcagtgttac tcattacaac tgccagttcg
     4501 gtaattgatt taagttctct gacattgcct ggaaactgat atgcaaggag tttgttctga
     4561 gcctttgcag atattttttt tacctgcagt tgattgtctt tacaaaactg tgtgataaag
     4621 tatttagcaa gtaagataat gtctttatct ctctcgcgca gtggtggtag atttatctga
     4681 agcccgtaga gtctgtaata caaatcttct ctgaacctgt tcttttttac ctcgtcaagc
     4741 aggtttttat gtgtggcagc aataatgcgt gtattaattt ttataggttt gttactccct
     4801 acccgcacca cttctttttc ctgcagcaca cgaaggagtt ttgcctgtaa ggacaattcc
     4861 atttctgcaa tttcgtctaa aaacaaagtg cctccatcgg cttcttcaaa cttaccaatg
     4921 cggctttgaa gtgctcctgt aaatgcacct ttttcatgac caaacaactc actttcggca
     4981 aggtcaatag gaatagcagc catatttatc gctacaaatc ttgacttttc ttttgacgaa
     5041 ttgtaatgaa tagcttttgc aatttcttct tttcctgttc ctgtttcacc tgaaatagtt
     5101 acagtgatgt tggtagccgc agccttttcg atgagtggaa acaacgactg aaaattgtta
     5161 ctgctgccta tcaacctatc ctgaaaattg taccttttct ctacctcctt ttcgaggcgc
     5221 gaaattttct gtttcagtcc ctgccgttct tttatcagct ctaccgtatt gagaagttta
     5281 tctcttatgt ctttcgattt aacaaaatag tcaactgcac ctgattttaa taactcaaca
     5341 acagtgtcaa tgctatgctg ttcggaaatg atgagtgtat cggcatcagg gcattgttct
     5401 ttaattagtc cgagcaaagt gcttccattg gtatccggaa ggtgatagtc aatggtaata
     5461 atgtcaggat ggagatgcag attcttcaga acttctttcc cattgctgaa agccgttaca
     5521 gcatgatcgg ggttcaaaga caacacatag tgcagaaact ctctgtacca gtcgtcatct
     5581 tctacaacga aaatgcgtgt aatggtggtg gttttcatct tacaaaagaa ataaaaaaag
     5641 gttgccgtta tgatgaagca accttttgga atgttgtccg actaggattc gaacctagaa
     5701 aggcagaacc aaaatctgca gtgttaccat tacaccatcg gacaaacgag ggcgcaaaaa
     5761 tagtataatt ttgaaaagaa aaaatcactt tttcaaaaaa actaaatgct gaaaattaac
     5821 cttttatgta agatttaaac cctttcaggt ccaaaccttg gttccttctg tacagttggt
     5881 acaaatgtca atattgcttc gtgacttgag taccaatttt ctgaaacggt tatatggctc
     5941 actatgccat acttttttga agccttctgt tgcaacctgc ccaaggcggt gacttgcatc
     6001 tttatcaaaa caacaaggaa caacattacc atcccaggtg atgacacatg aatgccacat
     6061 cctccagcaa tgattcaata atggattgcg gatactgtat gtgccgtctt tatttttttt
     6121 ataccgagaa tgtgaaagat cgtttggcat cagctcatta ccatgaacat aatcataaag
     6181 ctgtgctgtt ttataaacta cttcgtcaat accaatagat ttggctaact gctgaacttc
     6241 tttaatctga tgttcgttag gttttacaac taagaactga aaaatgagat gcggtgtttt
     6301 ggatttaagt ttgtttttcc atttaaccat gttttctgca ccctttatca ctttctctaa
     6361 cgatccgcca atacgatact gctgataggt ttcttgtgta gttccatcaa ttgaaataat
     6421 cagtctgtca agacccgact caatggtttt acgtgcattg tcatcatcca gataatgtgc
     6481 attggtggat gtagctgtat aaattttttt ttgtgatgca taacgtatca tctcaaacaa
     6541 atttttgttg agataaggtt ctccctgaaa atataaaatg agatataaca agtcaggtga
     6601 aagttcgtca ataacttttt caaaaaagtc aggttgtaac atacctgtcg gtctgctgaa
     6661 tgcacgaagt ccactcggac attcagggca gcgaagatta caggaagttg taggctcaat
     6721 tgaaatactc accggcatac cgctgacatt tgcttttccg gttatgcgtg acaagtaata
     6781 actacataga attttactga agttaaacag tcgtctgaca gttaacttac tcagtaaatt
     6841 tgatatgtct tttaaattac ggttcaatgg aatagaaaca aaactcgaaa aagtaaaaaa
     6901 tataatttac ttgttttcta tgacaaagta aacatttgtg caaagctaat gtttttatat
     6961 gcttatatta atcgagtcgt ttgtcggaag ttgtattaac gacattgcca ttttaagttt
     7021 tgtggcaacg gtaaggtaaa atgcatcgct tgatttgttt tggggtgaga aaaaagtatt
     7081 tcagatgcaa ttaaagcaat ggatccttgc tgataatctg ttactgcacc ataaaatgaa
     7141 tcgccaacaa caacagcgtt tctggaagaa aactgtgcac gtatctgatg gtaacgtcct
     7201 gtgattaatt caattttata gagaaaagcg tttgatttag tgttgcatac ttctttatat
     7261 cttaactctg ccctgacaaa accctctttc ttttttgctg aaagaattgc ctttttaagt
     7321 tttgtgtttt tctgatggta gtctgtcagt gtgccctctg cagcggttaa agaatgttct
     7381 gttaacgcat gataaatctt tgtaatactt ttattattaa actgttcatt aagattctgc
     7441 aaagccgatt ttgtttttgc aacaattatc aaaccttcag taacacggtc tatgcgatgt
     7501 ggaaagccaa tgtaatagtt tttattggtc gggaattttg cctgaagatg attcagaaac
     7561 caattttgca aagaaggatt tccaaaatta tcatcttcga ccattaaccc tgccggctta
     7621 tgcagcacgg caaggtaata gtcttcaaat aagattgatg gttgcaacag ttatctagtt
     7681 tattgaataa taaactttga tgtcagttta aatccgtcac cattggtgaa ataaacaaaa
     7741 tatattcccg gtttcaagtt ctctttgttg aagtctaaag aatttgactg caggtgttct
     7801 attctttgta cacaatttcc cagcacatca taaattaaca agttgccatt atcaactgta
     7861 aggtcgctac tgatagtaat tgttgcatct tgtaggacag ggtttggaaa aactgttaat
     7921 gcatattctg tttcaaatac atcaggctct cctgcgatac gacatggatt aaccttaacc
     7981 tgtactaacg gtgacagttt tgagcaacca tttccggttt ggcgaactct gtatccgcct
     8041 gttgtatttg ctgaataaga agttcctgtt tctcctacaa tatctatgct gtttttagtc
     8101 cactgatata caactcccgg ccatgagttt gtacttaaaa caacatcctg accagtacaa
     8161 attgttgttg gtcccgatgc tgtaattgta ggggttggat tattaattac cgttaacacc
     8221 aaatttgatg ttgtaacagg gcaaatgcca tttaaagaaa caatagcata gtaagtacct
     8281 gcagatgtga cattacaaga agagtttact gcaccattca gggcaacatt atttctatac
     8341 cactgataag tatatacagg atttgaagta acagataaat gaaatacttg tcctgcacag
     8401 attgtagctg ttccggaagg agaaataacg gctgaaacag tagcgctatt cacagtcaca
     8461 acattggaag ttgcattacc acaaggcgat gtcatataaa ctgaataact gccagcagta
     8521 tttacagtca aagtaggtga tgtttctccg ttaatattaa caccattgtt tttccattga
     8581 taggtgaagc ctgtacctgt acttgctgat aaaactacag aagcacctgt acaaatggta
     8641 actgttcctg caggaagtac agatgcactg aatgaaccta ttgataccgg tattgcattg
     8701 gaaattgtag taccacacga tgctgacacc ttgcatgtat aactaccact ggaagttgct
     8761 gcataagtac tgcttgtttc attaattatg tcagtaccat ttttttgcca ttgataggaa
     8821 tagcccggta tagggtttac caattccatg tttactgttc ctgttggcga acataaagtt
     8881 gtctgatcgt tggtatgaat ctgagctgtt acacttgtgg catttttaac actaaccgtt
     8941 tttgatttag agcagccgtt tgcagtttga ataaccctat atcctcctgc ttgtgttgca
     9001 ttgtaagact gtgaagttgc gcccggaata tcaacactgt taaactgcca ctgataattt
     9061 acacctgtaa atgtatttgc agtgagtgta acataacctg gtgcacaaaa tgataatgct
     9121 gttgatgaac tgatggttgg tgctggatta ttatttacac taatcacaat tacatttgaa
     9181 ttaccggaac aagcacctgt tactttacaa taatactgtc ctgcactgct tgcataataa
     9241 ttctgattga cagcagttcc taatgcaaca ttatttctat accactgata ggatgatgct
     9301 cctacaggag cagataatgc aacattggct ccggcacaaa tgtttgttga gccgtttgct
     9361 gtaatttgtg gtgatcctgt gccgagcgtt agtactgttg gtgatgaagt tgctgaacat
     9421 cctgataatg taacaacaac atcgtaattg cctgatatgc tagcagtgta attatcagca
     9481 gtggcacctg aaatattgtt gctgtttagt cgccattgat agctaaaccc aacaccagta
     9541 gcagcattta ataactgtga tgaattacat gttgtaacat ttcctgatgg tgtaattgct
     9601 gcaaccggca atgagtttac cgttgttgtt gcacttgtac ttcccgtaca tccattggca
     9661 ttggtaacag ttactgtaaa agttcctgct gttgatacat tgattgaaga tgttgttgct
     9721 ccggttgacc acaaatatgt actgtatcct gagcctgcat ttaatattgt tgaacttcct
     9781 gaacaaaatg ttgttgttcc gctgatggat ggtgttgggt tggaaataac cgttgttgtt
     9841 gcacttgtac ttcccgtaca tccattggca ttagtaacag ttactgtaaa agttcctgct
     9901 gttgatacat tgattgaaga tgttgttgct ccggttgacc acannnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnngttgttg ttgcacttgt acttcccgta catccattgg
    10081 cattggtaac agttactgta aaagctcctg ctgttgatac attgattgaa gatgttgttg
    10141 ctccggttga ccacaggtat gtgctgtatc ctgagcctgc atttaacgtt gttgaacttc
    10201 ctgaacaaaa tgttgttgct ccgctaatgg atggtgttgg gttggaatta actgttatgt
    10261 tactgttgtt gttacttcca attactgaag gattagaact aatcacacga actctgtacc
    10321 ctgcacctgt tgcagtgcct aaaggtatct gacaagtaat agttcctgca gtggtagcag
    10381 taagtgtacc aatgtttact gggctggcaa atgaaccgct gctattagaa agttgtgctg
    10441 taaaaacatt acctgcattg gcagcagcat caacagtata agaaacattt aatgatgcgc
    10501 ctttacaaaa acttaatgga gaaaccggtg atgtggttat ggttgttaat ccagttgatg
    10561 ttgtaatagt aaagttagta ttactgatat cgaagaaaat atttccaacc gcttcaactt
    10621 ttattcttgc agtagtagtt gaaatattcg gaactgtaat agtctggctt ccatcatttg
    10681 gagtagaaga taataatact gtcggatagg tataaccgcc atcagtactt aatgaaattt
    10741 ttacgttagc tgtattgata ccactacctg ttgttccact tacattccag gtgactgttt
    10801 gtgtactgtt gcctgcccag gttacagcag tgtttggagc agttactgcg aaagcgccac
    10861 ctgcgttaac aacggtaaca attaaattag agtcgggatg catgatgcca ccaccaccgg
    10921 tgcgactgtc acgtgctgtt aatctgaatt ttaatgtacg tgcctgattg ggcaacaact
    10981 cacctactgt tgtagtatta tttaccacat cagacatttg tggaaatgtg cgtgatgggc
    11041 ttgtggtagg tgggaaagga cgaaacattg gtgccgtagt tgactgtaca ttccatgcac
    11101 cggcaggacc caaatcgcgt tcttcccaag aatatgtcag cgcatcaccg tttgcatcag
    11161 tggcagcacc tgttagaaca aaaggtgtac tgataggaat tgaacagtta tagcccatat
    11221 tggtaactac aggagctgtg tttcctgttg atgtagtaac agggcatgag tttcctcctc
    11281 ctgtatttga aaaagtaatc atctgatcga ggctgtaacc atgaaaatag gctatactgt
    11341 gtggtgcaag atcatcgcta ccacaaattc ctgcataagc ctgaatagta ataccactgc
    11401 ccggttcata agctgcagta gaggatctgt tgccactgca acttcctgtt gaactgttaa
    11461 aagtatgatc gcctccaaac tgatggccca tttcatgcgc tacatagtca atatcaaaac
    11521 catcacctga tggagcacca tttcctgtta caccttttgc tttattggaa ctgctgcaca
    11581 cacatcctaa ataagccaca cctccaccac ctgtgctgaa aacgtgaccg atatcgtagt
    11641 ttgaactgcc tattatatta tcacaagtag tttgattctg actcaacata gttgaaccat
    11701 tgttgttggt atatgggtct gatgatgaat tagtataaac aatcagatta ttattggcta
    11761 ccataaccat gcgaatagaa agttctgatt cataaacacc gtttacacga ttcatggtag
    11821 taaccatgcc ggccattgcg ccagacactg ttcctccgta aaatgctgca tattcaccag
    11881 tacatgccaa tgcaagtctg taggtacgta attgcgtacc gttggtacgc tgtacggtat
    11941 taccatttgc attttgtatt tccattgctg tttcatcagc aagcaaacat tcaaaagaat
    12001 tttgtctgat tagattcttt ctttcataac tcatgtaata atttacagtg ttttgattgt
    12061 aagggtcaat ataaacatcg ccttttgaag aacgaatcat ggcatgaaaa ccaaattgtg
    12121 ttacatctaa ttttatggtt gccgtaacat cgtcaatacc ctgaccggtg taagttttta
    12181 tctgcggaaa tttagcagcc aaatcggggt gcattacatt gtattgaaaa atgctgaaac
    12241 gctcaaaaga gccatcaggc ataggtaact caagcatcaa tccgttttta agatttgctg
    12301 tttgtgaagg ctctgctttt gccgtgttta aatattgctg taataaattt aaatcaagat
    12361 taagtgccct ataacttgaa ggcacagtaa gtctttcacc ggaaacagga atagttgaca
    12421 cctcgcgcca gaaaattttt gaatcttgtg cgttgaggaa ataggttgcc tgaatgatga
    12481 acaatgccgt catcattgtt ttgaaaatac ccttcatttg aaaatcgttt tttattatac
    12541 tttgaagcaa aagtccttaa aaaaacaatt aataacaaaa gccggagtaa taaatgcaca
    12601 aatagcagta tagtaaacga gaagctatta ccgttgcgga gtttcgattg aataagccgc
    12661 caactgacat taataaaatt ataaaaaatt tatttccagg ctttcacgat aagaccggta
    12721 cctgtaggat gttttatgtt tacatctaaa tagtttaaca caaaacaaat tggataaaac
    12781 agcaggtaat agaatggaag aataataaaa aacgccttgg tatatcctaa tgcagagata
    12841 ggatatttca tggacaaacg ccaggatatt tttccgggag taccataaga gtactttgct
    12901 tcaacacggc tgaatccatt tcgcagcagt tttactttta tatcatcaat attataacca
    12961 tcacggacat gctcttcgat aaaagattgt tgatcatcgc catgaacatc gctaccgccc
    13021 tgatcacttg gtgttgaaat gactaacatg ccaccggctt tgagtgatgc tgcataattt
    13081 ttaaatactt gctcatcttc cagaatatgt tccattacat caacacaaac aacaagattg
    13141 tatgttgaag gtgattggta cttggttaaa tcttcaatgc cgaattgtac gtttttatat
    13201 cctgccttct gaaaaaaagt attgcaatcg ctcacctgct cttctttgac atctactgca
    13261 taaattttcc aatttctgtt tcgtgaagcc agccagtagg tatattgacc aaagccggat
    13321 cctgcatcta aaatatgtgc attgtcgcca actgtacttt cccattgctt tagctcttta
    13381 tgaatatgcc aggcacgcaa aagaagtaaa tcgagcagac ggtaaaatgt ttttcttaaa
    13441 aatggtgatt tattaaacac ctcgcccaaa gagcgtttta taggatcgta ttgcattcag
    13501 tgtgtcagcc ttcctttgga tcgtttttat ggatttcctt aaacagcttt tcaatatgct
    13561 cggcatagtc taaactctca tcattaaaaa atttcagctc aggtatatgt cttatctgat
    13621 tgcgtattct gttaccaagc tttcttctga tgtcgtgcat tttatggttc aggttgctta
    13681 ctatctcttc agcatttttc tgcttgaata aacttaaacg cacataagca atgccaaggt
    13741 caggtgttac tctgacattg gtgacagtaa caaaggcatt tccataaaac gaactgcctt
    13801 cgagctgaaa caaactactg atttcttttt gcaacattct tgaaattttc tgttgcctag
    13861 tgctgtccat taattcattc ttaaaataat acttatttga tttcgcccaa cttattggtg
    13921 agcagctgaa tggctgtgcg ctgattgtca atttcgcttt tcttcttgct caggtcattt
    13981 tcttttttgt aaacatcatc ttctgccttc ttgatattgt ctttatattt ttcaatatcg
    14041 ctacgcaaag tcttaatgtc tttttctaca gagcctctgt cactttccat cttacctaac
    14101 aatttttctg catcttttag ctgtgattga attggtgcct tggtagtttc aattgcaaaa
    14161 tttttgacca tctgttctgc tacaccgtat ttagtggcat caactgttga tgataaataa
    14221 gcaccgccta aatcaaatgc taccatcact ttaacgctgt tgtctgactt gttttcttca
    14281 aaacgagcgt aaatgtcaac agggttattg ccccaatctt ttatcaatac attatcggta
    14341 aatgtttctt ctttacctgc tgttactttt ttacttttta atgacttcat ataggttgtc
    14401 cagctattgg tcacatcctt gaggttgttg ttaaagcaag ttgtaacgat tgctggttgc
    14461 tgtccggtag agaatgttga ggtttgttca gctacagtaa taacctgtgc cgaagtaatt
    14521 gtagtcgcaa aaaacaatgc tgccgctaaa atctttttca taaattttct gattaaaaat
    14581 aataatgcaa aggtagtaaa aatgccgttt cgcactgaat aaataatgat aaaaatgtat
    14641 aattttatag aggtcgcccc gatgaggctt ttaatttgaa agaaagttgc ttagatgcgg
    14701 tttctggaaa taatttataa taaaataaaa aatccaccgg gttgtagtaa aagatgatta
    14761 gcctaatttt taaacattat gcaattgttc taaagtttat caagtcttac tctataggca
    14821 tatcccactc tcaccatcca tgaattctga acactcaaag tactttgggt atctatgttg
    14881 tgattcttag tgcctaaaac aaattttctg aatacagaat gtccggtctc taaaaacaac
    14941 acattttgtt tgtttacata aaagtctgct gctattctaa tcaggttgtc attggttctt
    15001 aggtagtaac tgtcgtcctt gcgatatgaa agtgtcattg ttttaagcga aattataaaa
    15061 tgtagtttgt tgatgatggc cttatactct ccattgagcg aagatggaag aataccccat
    15121 aaattccatc gtggtgttat gcgataatca ataccaagca gtggcaaaaa gtaaggtcca
    15181 aaaaattcgc tgttgtaata caatccaagt ttatagctaa gttgttccga gcgctgatag
    15241 gtttgtaaaa tggctcctcc aatctgataa ctgtcttttt tcaaccaata tttactttca
    15301 gaaatagatt tcggaattac cactaaagca gttttaaaac gtttgtcttt ccactgatgt
    15361 tgccatgtta atgcaatctg accattcata taaagtttag attgctgacg gaatttaaaa
    15421 tcgaacctat caaaagacgg attgatgata aaaatattat tgccgctcct tattggaagt
    15481 gtaaggaaag cattggtgta ctgaagcgtt gcagattcgt tgttggtgaa ctttaatggg
    15541 cggtggtaac tatagtttaa tgatgcaatg tcaattaatg gttgtgccac taccgactgt
    15601 gcaaacaata aaagtaatat aactataagg cgcatattta cagtttatcg aatgcaagat
    15661 taaggcttgg actttaaatt accaaccttg acaacctgat aatttttact tttgttgtac
    15721 ataacatcag actatgttta atattctaaa aaaattactg attctactat ctttaccatt
    15781 agctacaaat gcacagtcgt ttaaagcttc tcttattggt ggtatcaatg cttcacaagt
    15841 ttccggtgat gaattgggag gttttaacaa agcgggagta atgtttggtg gcagtgccat
    15901 attaccggtc acgccaaagt ctgatgttgc aatggaatta ttgtttattc aaaaaggaag
    15961 taaaacacca acatcaaaag ataacaacaa tgccgactat tataaaatga gtttaaatta
    16021 tctggaagtg caaattaatt atacttatca tgcatcaaaa aaaataggca tgcatgttgg
    16081 gcctacgttt ggtgttttga ttggtgagaa agaagaagat attgcaggtg agttaacaga
    16141 aagaccggag tttcaaaaga cagaaatagg cattgcaggt ggactgtcat ttcacttcag
    16201 cgagaaagtt ggattgtata tgcgtttatc aaattctttg ttgccaattc gtaaaatggg
    16261 agcagacaca cgttacttca aaagcggaca atacaatagt ggtctggcat tttttcttac
    16321 ctacactttt agctcaaaag acaaacaaaa gcaataatga aaaacaaaat agtggttgcc
    16381 attaccggtg ccagcggaag tatttatgca tcaatattgc ttggcaaatt ggcacagctt
    16441 aaagaacaat tttctgatgt tgcaatagtg ttttcaaaaa atgcaacaga tgtgtggcag
    16501 cacgaattag gtaatgatac ctataagtcg ctttcgttta agcgatatga caccatggat
    16561 ttcaatgcac cttttgcatc cggatcggca caatacaaca cgatgattat tattccttgt
    16621 agtatgggga ctatgggacg cattgctgca ggaatttcaa acgacctgat tacacgtgct
    16681 gcagatgtaa tacttaaaga gcacagaaaa cttattttgg ttacacgtga tatgccttta
    16741 aatctgattc atatcaacaa catgaaaacc attactgaag ccggaggaat aatttgccct
    16801 gcatgtcctt cttattatag taaaccacaa actatcaatg atgtggcagc aactgtagtt
    16861 gaccgtgcat tagaccttgc cggttttagt ttgaacagtt tccggtggaa tgaataaaca
    16921 atcttctgca tttgaaatga tttcaaatct tctttttatc agactgcagc aactgaaacg
    16981 cgaattgata agtacgggat ggttttacag cctgataata ttaatcatta ccggtctttt
    17041 ggggttttta ctttatcatg cagtgcaaaa acaaattcat agtttttatt tggcagcatt
    17101 gataattgtt gctgtatata ctattcataa ttcaagaaat gatttgcagt ttatcagaca
    17161 acaattaaga aatccatttt ttctattgac aactgaatat attttgttag cacttcctgt
    17221 tatctttcta ctcttattaa aaggcttttt tgcagcagga tgcacaataa ttacaggatg
    17281 tgtattggtt gctttaatag ctcccaaaaa ttcagtgtat ttactttttc caaaacttgc
    17341 aagttggata ccttcagaaa tgtttgagtg gatgagtggc atacgaaaaa cgaaaggtgt
    17401 tgtcgcagtt tttataattg taatgtatgc aacttctttt ttaaaattta ttccattgat
    17461 atttctttgg ctgataacaa taaccattgc cgggttctat caatttggtg aaccacttac
    17521 actactttat ttgcatgaaa atgccaaacg tcttctaagc aacaaaatta aaagtgcttt
    17581 gtcgcttttt gtaaagatat ccatcattcc ggttttggtg aatatatttc tccatcctga
    17641 tatttggtgg gttgacttaa tggtttacac tttatgcctg cttgttatta cttgtgctat
    17701 acttattaaa tatgcagttt acagacccaa tgaaagatta actgcaggaa gtattttgct
    17761 gtcagtcgtt tgtatttcac cgacactgcc attcacaatt cccattcctg ttgttgtggc
    17821 agcaatgaag tataaaagtg caattcaaaa cttagaaaac tactttcatg atcagaatca
    17881 ttaatctgaa aatttctttt gataagcatc aggtgcttga tattgaaaaa tataatttta
    17941 ctacaggaaa agtacatggt attattggtc tcaatggtgc cggcaaaaca acctttttta
    18001 atgctgtatg taaatttcta aaacgtgatg cgcaagccgt tttgataaac aataaaccat
    18061 ttgagcgtca tgatgcagcc tatttggaaa ctacaaatta cttttatgaa aacattactg
    18121 cacaagaata tctttcgctt tttccgtcag acaatttact tttcaataaa aataatttga
    18181 ttgaactgct tcaattaaaa tcctgtgggt tgattgaaac tttctctaca ggcatgagaa
    18241 aaaagctggc tttgattgca ataatctgtc aggaaaaacc ggtatatata ttagatgagc
    18301 catttaacgg attagattta gagtcgaata agattttgga aatgttaatc caaaaacttg
    18361 ccgaaagtgg taaaacaatt tttctctcat cacacattct ttctccttta acaacatcat
    18421 gtactgaaat acatctgctt cgaaacggaa attttgaaaa acgttttcaa cctgaacagt
    18481 ttaatgaaat agaaaatgag ttgttttcta atctcaccga gaaagtcaat tcgattctaa
    18541 aaaaataatt tatgcagtta ctttttcgaa tgctgcttta agcaactgtt gttgttcctc
    18601 atcatgagca ctgtgagatc cggttgcagg gct
//
LOCUS       DAOQNP010000065        17753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1120_length_17753_cov_7.816307, whole genome shotgun sequence.
ACCESSION   DAOQNP010000065 DAOQNP010000000
VERSION     DAOQNP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17753
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_17753_cov_7.816307"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1191)
                     /locus_tag="PKY09_09330"
     CDS             complement(37..1191)
                     /locus_tag="PKY09_09330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB13099.1"
                     /translation="MSSRKKILIAIDWYLPGYKAGGPIRSCASIINCLKNDFDFAVIT
                     SDTDFSDDKPYEGINSDAWNILPDGTRVYYFSKNNLSQKSFREVIQKEKPDILYMNSF
                     FSQNFTLKPLKIARKYLPECKCIIAPRGMLGAGALAIKPLKKLAFISLSKMLATFDKV
                     RWHASTEQEAKEIKAIFGNRAEVRVALNLSSVKPAVAETKIKNAGHLNLVFFSRISKK
                     KNLHLVLEWLKAIPQHLKIIFHIYGTLEDDAYWSQCNDLIKLLPQNIAVEYKGVVDAT
                     HSESVFENNHFSILPTMHENYGHAIVESLAFGVPVIISNNTPWHGLEAKFAGFDFPLD
                     NQQHFIESLIKSASMDQETYKVWQHGAMELAKEVLNNSDTVNQNKKLFAE"
     gene            complement(1191..3059)
                     /gene="asnB"
                     /locus_tag="PKY09_09335"
     CDS             complement(1191..3059)
                     /gene="asnB"
                     /locus_tag="PKY09_09335"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB13100.1"
                     /translation="MCGITGIYGMSSGQSSSSIRLMNNAISHRGPDDEGYYVDEQVAL
                     GHRRLAIIDLSPAGHQPMHSNDGNLEIAFNGEIYNFNDVKKLLTGYAFKSGSDTEVIL
                     AAYQQWGKDCLHHLNGMFAFAIWDKSKQELFITRDRLGIKPVYYFSKDKMFLFSSEIR
                     ALLQSGIVPRKINRRVIHEYFTYQTIHAPETFINNVFMLMPGHCMTINASGINIERYW
                     HIVENTDFSSSVKSYREVCADINKLFFDAVNRRLISDVPFGAFLSGGIDSSAVVGAMS
                     RIHTQPVKTFTIAFDESDFSEAKFAKLIADKFKTDHHEFLLKPEDFLNQLPEAMNALD
                     HPSGDGPNSYIVSKITRQAGVTMALSGLGGDELFAGYPVFKRTELLYQKSWLWKLPLP
                     LRQLLGKLITALKKDTASIKLQQLLLSPDASLQYSFPVSRQISNAQQLSLFIEEKFNG
                     IDAVKEIIKELNKSSAGLPLFSQVSAAEISSYMQNVLLRDTDQMSMAVALEVRVPFLD
                     YKLVEYVMGVNDEYKNPVYPKKLLVDSLGDLLPDEVVHRKKMGFVFPWERWLKKELHA
                     FCGDRIYALADRGFMNKEALTDRWQSFNEGKPGVRWLDIWLCVVLEHWLEKNNIEY"
     gene            complement(3063..4133)
                     /locus_tag="PKY09_09340"
     CDS             complement(3063..4133)
                     /locus_tag="PKY09_09340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB13101.1"
                     /translation="MQTAKKKALFLYLEPAGYVEACFKKTAQNYPVELHVVKYPLDPN
                     APFRFDDVANITYYDRNQYQEQKLNDLVASINPDFIFCNGWMDKGYVNVAKQFSGKIP
                     TVLAFDNLWEGKLKQYIASVTAPFILPRIFSHCWVPGQPQVVYAKKLGFKENRIETGM
                     YSANYELFDQAYHKLMPIKEKKFPKRFIFVGRYIEIKGIRELWQAFTQLQNEMPNEWE
                     LWCLGKGPLEKEFPQHPKIRNVGFIQPSAMEEYMRQTGVFLLPTYDEHWGVVVHEYAI
                     AGYPLLLSTKAPAGTAFLKQGENGYYHEPSNVESLKEALKKIMLLPDSTLIEMGKKSH
                     ELSKSMTPDTWAAVVWKILNSK"
     gene            complement(4093..5412)
                     /locus_tag="PKY09_09345"
     CDS             complement(4093..5412)
                     /locus_tag="PKY09_09345"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13102.1"
                     /translation="MLQFIRNNAQFVIMCFAWVFCGMVNETLGVAAVALSVVMLKLKG
                     MYKELILGLFFLLVMSDSRQSEALYAAQAKNIYIVLLSLFYFFDRKNFTGTNDYFKPF
                     ILFFIWSAAMLMQAPSVNFMNSAMKTLSYILLFTVVPAYFIKAFRELGESFIKEVIML
                     FCCILLIGIVMIPLFPNTVFLVGRFCGVYGNPNGIGIACTLLFMLVFISLKKFPGLLD
                     QNETRIVYGLIILSTLLAVSRNTMMSIVIFLLFARFYEMGYLAGFAILVVALVAYELI
                     NQNIVAIVEALGLGHYLRAEAIEDGSGRLIAWRAAWKQIQSQFFLGRGFAYDEWYFYE
                     NRHWLSALGHQGGVHNSWLAIWMNTGLIGLILFAIGFIKTFVKAMLHSKLAFPVLFAV
                     IFSASFEAWIMGSLNPFHIVLILILNFIINIDQFNADREEESVVPIS"
     gene            complement(5442..6575)
                     /locus_tag="PKY09_09350"
     CDS             complement(5442..6575)
                     /locus_tag="PKY09_09350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB13103.1"
                     /translation="MSQPVQVVFCTDGVYPQLVGGIQKHTRLLVEELARTKKIKIAVI
                     HQHHEQNVFDASLNITEIGIKRNKHSSNYLLNEYYYSKQVYEALKQFPDAVIYAQGMA
                     VWHNIDKIGSRVILNPHGLEFYQALSFSDRLKAIPFKFVFGYLFSKAAKVVSLGGRLT
                     QLLKSNVKSHHNKIVVLPNAINLPAQKFERSVFDKRPIKLLFVGRFAANKGIHVLMDA
                     AAQLNNGGYKNELEFHLVGKGPLFEHFSTTVKEPNVVFHGFADDDKLNSLYKECDIFV
                     LPTLFEGMPTVVLEAMSYSLPIIVTDTGATTVMVDSLNGYIIEKSDVKSLKDAISMYF
                     VLNEQAKKRLAEQSYLRVKEKFTWPVIAQQHLELFQQMAEQKT"
     gene            complement(6572..7477)
                     /locus_tag="PKY09_09355"
     CDS             complement(6572..7477)
                     /locus_tag="PKY09_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB13104.1"
                     /translation="MKRILVTGGAGFIGSAVIAHLQKQKHEIFVIDNLSFGNRQFINI
                     DDSHFFNADILDEQRLDEIIGKIRPQYIIHLAAVHFIPYCNQHPYESSNINIRGTMHL
                     LKAAQKYTPEKVFFASTAAVYPIYDLAVSESFATGPMDIYGLSKLTGEHLCNEFFLET
                     DIPVIVCRFFNAFGPNETNPHLIPEIQKQVLEGKRVIKLGNLTPKRDFIHTYDMAAAV
                     ETLLTHVEGGIHTYNLGRGIEYSVTEIVDAFSKAINEKIEIEVDQSRVRKVERQHLLA
                     DVSKLKSLGWTPKIGIEEGIKTLVQ"
     gene            complement(7547..8677)
                     /locus_tag="PKY09_09360"
     CDS             complement(7547..8677)
                     /locus_tag="PKY09_09360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB13105.1"
                     /translation="MIYLTYADQPSGVYSSQVVDVCNFLNESHNAQIRLVSFISLHNF
                     SHNRQKIKREMPSAIVLPMLPRMTSFGFNTLMLFFVLLFLNQRSVIARNVLAARIALR
                     LKSILKLKVCFDGRGAIAAEWNEYQVVPLQEWKNSIHTWEKSVVLNADFRIAVSEELV
                     KYWNERYGYSENKHVVIPCTLNTSFRPEILKQEEIENARKKMKFNADDIILVYSGSTA
                     GWQSFDLLQGFLQKIINNNKNIKVLFLSEKDKNVDQLKAEFPDKIFQTFVKHSEVSGI
                     LKSCDIGILIREKSVTNQVASPTKFAEYLSAGLPVIISEGIGDYTSFVKQNDCGWVLD
                     ESPLQMNRLNISERNRLLQLVNDHFTKKSHNASYTNLIQLMT"
     gene            8928..10034
                     /gene="lepB"
                     /locus_tag="PKY09_09365"
     CDS             8928..10034
                     /gene="lepB"
                     /locus_tag="PKY09_09365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNB13106.1"
                     /translation="MNWKFWKSSGRKSKTREWIDALVFAVVAATIIRTFLIEAFTIPT
                     PSMEKSLLVGDFLFVSKVSYGARTPMTPLSFPFVHQELPIIGGKSYSDAIKWGYHRLP
                     GFGNIKNNDVVVFNYPGNVFYPDDDLKRPIDKKTHYIKRCVGIPGDSLKIIDGLLYVN
                     NKPVPLPEHGQMTYTVITDGNTINPKTLEKYNIYEGANLGNGIYQFTLTKEIADELKK
                     LPFIKSVELYNQLPGAPSQFQKLFPFDSRYNWNIDNYGPIWIPKAGNTITLTEANLPF
                     YQRCIVEFENNSLVIKGNQFIINGTPTTTYTFKQDYYWMMGDNRHNSADSRFWGFVPE
                     DHIVGKAVFIWLSWNSNGTFFDKIRWGRLFNFIH"
     gene            10034..10615
                     /locus_tag="PKY09_09370"
     CDS             10034..10615
                     /locus_tag="PKY09_09370"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNB13107.1"
                     /translation="MSIAVFSTAYFPSLEYIRHILQFDEIIIETKEHFPHQTNRNRCH
                     ILGPNGIQTLTVPIKQNHIKTPTGNIEICSGKWKQQHWRSLTTAYNRSALFEFYKDEL
                     ESIIFDNHRLLIELNTKILSFILKAKKKTIAINFTTSFQASYKNDFRYISDAKSQKQI
                     SDLNIPSYPQVFSEKIGFVSNLSAIDALFNGSL"
     gene            10677..11903
                     /locus_tag="PKY09_09375"
     CDS             10677..11903
                     /locus_tag="PKY09_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNB13108.1"
                     /translation="MNKKKIINDPVHGFITIPSEFIFDLIEHPYFQRLRRIRQLGLAQ
                     YVYPGAVHTRFHHAIGAMHITQIAIETLRNKGHEISATEEEAVSAAILLHDIGHGPFS
                     HALEHTLVAHLKHEEISMMIMEKINESMGGALNMAIEIFKNVYPKKFLHQLVSSQLDM
                     DRLDYLARDSFFTGVSEGIISSERILKMLNVFDDKLVIDAKGIYSVEKFIVARRLMYW
                     QVYLHKTVLAAENQLINTLKRARYLTAKGHDLFGTKSFKIFLKNNFTSKDFKQQPHLI
                     DEFCLLDDFDIFTSIKEWMTHDDFILSHLCKGLIDRNLFKIKLQEKSFTQQETENYLK
                     KAVKAYNITEEEASSLAFTGKIENSAYTKSQENINILFKDNVIKDIADASDNLSISAL
                     SKPVVKHYLCVDSGCL"
     gene            complement(11915..12520)
                     /locus_tag="PKY09_09380"
     CDS             complement(11915..12520)
                     /locus_tag="PKY09_09380"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNB13109.1"
                     /translation="MLKSYFSDDVEAGIDEAGRGCLAGPVFAAAVILPRDFSNAQLND
                     SKKLTAAQRDKLRLVIEKEALAWAVASCTHSEIDKINILQATYKAMHNAISKLEIFPE
                     RLLIDGNRFKNYKAVPHHCIVKGDGLFASIAAASVLAKTHRDEYMLQLHHKHPVYCWD
                     RNKAYATAIHVEAIKKFGLSPYHRKSFQLKSLQPQLIFPEN"
     gene            12617..14674
                     /locus_tag="PKY09_09385"
     CDS             12617..14674
                     /locus_tag="PKY09_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNB13110.1"
                     /translation="MPIQFVYPFFLWFLAALSIPIIIHLFNFRRFKKIEFTNVRFLKE
                     IKEQTQSHSKLKHLLVLLMRLMALAFLILAFAQPFVPTKSTESSGQQIVSLYIDNSFS
                     MNLSGKEGSLLDEAKDKCRTIATSLPSSTKFQLLTNEFKGEQQRLIDRDELLELTDHI
                     KINPQSRNINEVLIRQTDALKKSNSDNRSSFIISDFQKSFFDMQPGVNDSLLKLNLLK
                     TDAEEVKNIAIDSCWLSAPVIQLNTPSELNVKVRNYGNEDAEVPVRFYINNSQKTVAS
                     AKINAGMDATVKMNFTIIQPGWQLAKVEIDDNPIAFDDTYYLSFRIADKIPVYHIGSS
                     KNIFIDKLLANNSLIDYTFSTQNAVDYAMLKKCNAVILTDLKDISSGMSDEIARFVDL
                     GGTLIIFPDSNITPNGYENLFGKLNVENYSTLNTNSISVSKLDLANPLFHDMFEKTDE
                     RMSLPTVNQSYETVSSLRSGKNIVMQLQGGHSFLNYYHRKSGSVYQFTSAAQPAAGTF
                     VTHALFAPVLFKTILLSAPSSQPTLFIGENSDIKIPSSIQTNDVLHLINSDTKTDIIP
                     EFKTTNDGTLINLTGIEQAGFYQLKNLNDSILSIIALNYNRKESDLKAFSDEELKSMY
                     GNGKFKSVVVVNPTGSGLTKFIKQYSSGISLWKYCILTTLLFLIAETLLLRSDRKMVT
                     LKN"
     gene            14679..15944
                     /locus_tag="PKY09_09390"
     CDS             14679..15944
                     /locus_tag="PKY09_09390"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNB13111.1"
                     /translation="MDIILKSVRIINPGTTHHQKTRDIIIVNGKIKSITTNAGNEEGI
                     KELDLKGCYVMPGWFDMRANFREPGEEYKEDLGSGIKAAAAGGFTEVLLMPSTLYPIQ
                     SKSDTEFILNKCRNSLVNVHVAGCLTTDRKGIDMAELYEMYVSGAIAFTDDKKVNDPG
                     MLVRALQYSNTFGARIISYADEPSLSNNYLINESPSSVRTGMKGCPAVAEEIGLNRII
                     RIAEYTGIPVHISGISTAGSVVQIRNAKSKGIKITAEVNAHNLMLDETLVESFDSKYK
                     VKPVLRSRTDIQALKKGITDGVIDCVCSDHSPQDIEVKNCEYDYAAHGISSIETTFAT
                     LSTAFKGQLDAEILFNIFSGNPRKILGLEMPSINEAETANMTVYHPDKTYIFSEKEMF
                     SKSNNTPFHGTEFIGKVIGVVNNNKLSGF"
     gene            complement(15941..16696)
                     /locus_tag="PKY09_09395"
     CDS             complement(15941..16696)
                     /locus_tag="PKY09_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB13112.1"
                     /translation="MKLTSLIIEDDSMQAASLKKMVETACPEITVLDICFTIPQAEAA
                     IRKMKPQFIFLDIMMEPFTGFDLLNRFVEIDFEVIFTTTYQDFAYKAIKVSAVDFLLK
                     PFGEEELKLSVKKLKEKIASKQTMDYLGLLMQNIQKKPEAMQLAIPVLTGYRFVEVSQ
                     ILCVESSGKFNQVFLINGDDFISTITLAEIEKLVPDIMFFRSAKNFIVNLSYVIEYLK
                     NQEKIILKNGKSAPLSRLKKNSFFAAINRFQNS"
     gene            16839..17606
                     /gene="rsmA"
                     /locus_tag="PKY09_09400"
     CDS             16839..17606
                     /gene="rsmA"
                     /locus_tag="PKY09_09400"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016421228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNB13113.1"
                     /translation="MVKPKKYLGQHFLTDKNIANTIVDSLTLSQYSQVIEIGPGMGVL
                     TEELVSRFAGKLTLIEIDEESAAYIKQHFDSSKFRLLQEDFLHADLNNIISVPTAIIG
                     NFPYNISTQILFKVYEHRNMIPEVVGMFQKEVAERICSATGKKAYGIQSVLLQSCYDC
                     EYLFTVEPNVFNPPPKVKSGVIRLKLNSTKKIVSKESDFRRVVKAAFNQRRKILKNAL
                     NQLTSVMAEKGEMPFGSKRAEELHWTQFDELTQWFYK"
BASE COUNT         5955 a         3345 c         2985 g         5468 t
ORIGIN      
        1 ggacttttac ccattataca aaaataggtt gcaaaatcat tctgcaaaca attttttatt
       61 ctgatttacc gtatctgaat tatttaatac ttctttagct agttccattg ctccatgttg
      121 ccacacttta tacgtttcct gatccataga agctgatttt attagacttt caataaaatg
      181 ttgttgatta tctaatggaa aatcaaaacc ggcaaatttt gcctcaagcc catgccaagg
      241 ggtattgttg ctaataataa ccggaacccc aaaagccaga ctctcaacaa ttgcatgacc
      301 gtaattttca tgcatagttg gcagaattga aaaatgattg ttttcaaaga cagattcaga
      361 atgtgttgca tcaacaactc ccttgtattc aacagcaata ttttgaggta acaatttaat
      421 caaatcgttg cattgactcc aataggcatc atcttcaaga gttccataaa tgtgaaatat
      481 gattttcaga tgctgtggaa ttgctttaag ccattccaaa acaagatgca gatttttctt
      541 tttagaaata cggctaaaaa ataccagatt cagatgaccg gcatttttaa ttttggtttc
      601 tgcaacagca ggttttacag atgataaatt tagtgctacc ctaacttctg ctctattacc
      661 aaaaattgct ttaatttctt ttgcttcttg ctctgtgctg gcatgccatc gcaccttgtc
      721 aaaggtggca agcattttag aaagagaaat gaatgcaagt ttctttaatg gcttaatagc
      781 taatgcacct gcacctaaca tacctcttgg agcaataata catttacatt ccggaagata
      841 tttacgggct atctttagtg gttttaaagt aaagttttga gaaaaaaagc tgttcatata
      901 caatatatcg ggcttttctt tttgtatcac ctctcgaaat gatttctgac ttaaattatt
      961 tttagaaaaa taataaactc gtgtaccatc aggcaaaata ttccatgcat cggagttaat
     1021 accttcatac ggtttatcgt cactaaaatc ggtatccgaa gtaataacgg caaaatcaaa
     1081 atcatttttt aaacagttga taatactggc acaagaacgt attggtccgc cagctttata
     1141 gccaggaagg taccagtcaa ttgcgatgag aatttttttt cttgaagaca tcagtattca
     1201 atattatttt tttcgagcca atgttcaagc acaacacaaa gccaaatatc gagccagcgc
     1261 acacccggct ttccctcatt aaaactttgc catctgtcag taagagcttc tttattcatg
     1321 aaaccacgat cggcaagtgc gtaaatacgg tcaccgcaga aagcatgcaa ctcttttttc
     1381 aaccatcgtt cccaaggaaa aacaaaaccc atttttttac ggtgcacaac ttcatcaggc
     1441 aacaaatctc ctaaactatc aactaataat tttttaggat aaacagggtt tttatattca
     1501 tcattcacgc ccataacgta ttctaccaac ttatagtcaa gaaaagggac tctaacttca
     1561 agtgcaacag ccatactcat ttgatcggta tcgcgcaaca acacgttttg catatagctc
     1621 gatatttctg ctgcactaac ctgactaaac agcggaagtc cggcagaact tttattcaac
     1681 tctttaatta tttctttaac agcatcaatg ccattaaact tctcttcaat aaataatgat
     1741 aactgttgtg cattacttat ttgccttgaa accggaaatg aatattgcaa agaggcatca
     1801 ggtgacagca acaactgttg caacttaatg cttgcagtat ctttctttaa tgctgtaatc
     1861 aacttgccta acaactgacg aagtggtaat ggcaacttcc ataaccagct tttctgataa
     1921 agcaattctg tacgcttaaa aacaggataa ccggcaaaca actcatcacc tccaagtcct
     1981 gatagtgcca tagtgacacc ggcctgccta gtaatttttg aaacgatata tgagttgggg
     2041 ccatcaccac ttggatgatc aagagcattc attgcttcag gcagctgatt taaaaagtct
     2101 tcgggtttta gcaaaaactc atgatgatct gttttgaatt tatctgcaat caatttagca
     2161 aattttgctt cgctgaaatc tgattcatca aaagcaatgg taaatgtttt taccggctga
     2221 gtgtgtattc ggctcattgc acctacaaca gcactagagt caattccacc tgaaagaaat
     2281 gctccaaaag gaacatcact gataagtcgt ctgtttacag catcaaaaaa taatttattg
     2341 atatctgcac aaacctcccg gtaacttttt actgaagaag aaaaatcagt attttctaca
     2401 atatgccaat aacgttctat attaattccg gaagcattaa ttgtcatgca atgcccgggc
     2461 atgagcataa aaacattatt gataaatgtc tcaggtgcat ggatggtttg ataagtaaaa
     2521 tactcatgaa taacccttcg gttaattttt cggggcacaa ttcctgactg cagcaatgct
     2581 cttatttctg agctgaacaa aaacatttta tccttgctga agtaataaac tggctttatt
     2641 cctaatctgt cgcgtgtaat aaaaagctct tgcttggact tatcccaaat agcaaaagca
     2701 aacatcccat ttaaatgatg caaacaatct ttgccccatt gctggtatgc cgcaagaata
     2761 acttctgtat cactacctga tttaaaggca tatcctgtaa gaagtttttt tacatcatta
     2821 aagttataaa tctcaccatt gaaagcaatt tcaagattcc catcattact atgcattggc
     2881 tgatgacctg ccggagaaag atcaataatt gctaatcttc ggtgaccaag tgcaacctgt
     2941 tcgtcaacat agtatccttc atcatcaggg ccacggtgtg aaatagcatt gttcatcagc
     3001 cttattgagg atgacgactg tccgctgctc attccataaa tacctgttat tccacacata
     3061 atttatttac tgtttaaaat tttccagact acagcagccc atgtatcggg tgtcatagac
     3121 tttgacaatt catgactttt tttacccatc tctattaaag tactatcggg caaaagcatt
     3181 atttttttga gtgcctcctt cagtgattca acattgcttg gctcatgata atatccgttt
     3241 tcaccttgtt ttaagaaggc agtaccagca ggagcttttg tgcttaacaa caacggataa
     3301 cctgcaatgg catattcatg aactaccaca ccccaatgct catcgtaagt gggtaaaaga
     3361 aatacccctg tctgacgcat gtactcctcc attgcagaag gttgaataaa acctacattt
     3421 ctgattttag gatgttgagg gaactctttt tcaagtggtc ctttacctaa acaccacaac
     3481 tcccattcgt ttggcatttc attctgcaac tgcgtaaatg cctgccacag ttcgcgaata
     3541 cctttaattt ctatataacg tccaacaaaa ataaaacgct taggaaattt tttttctttt
     3601 attggcatta atttatgata tgcctgatcg aacaattcgt aattggcaga atacattcct
     3661 gtttcaatac gattttcttt gaagccaagt ttttttgcat aaacaacctg tggctgacca
     3721 ggcacccagc aatgtgaaaa aatgcgaggc aaaataaatg gtgctgttac cgatgctata
     3781 tattgtttaa gttttccttc ccacaaatta tcgaaggcaa gcactgtagg tatttttcct
     3841 gagaattgtt tagcaacatt tacataacct ttatccatcc atccattaca aaagataaaa
     3901 tcgggattta ttgatgctac taagtcatta agtttttgtt cctgatactg atttctgtcg
     3961 taataagtaa tgtttgcaac atcatcaaat cggaatggcg cattggggtc aagcggatac
     4021 ttaacaacat gcaactctac cggatagttt tgtgctgtct ttttaaaaca ggcttcaaca
     4081 taacctgcag gttcaagata taggaacaac gctttcttct tcgcggtctg cattaaattg
     4141 atctatgtta ataataaaat taagaattaa aataagcact atgtgaaatg gatttaagga
     4201 gcccattatc caagcttcga acgatgctga aaaaataaca gcaaacaaaa ccggaaaagc
     4261 cagtttagag tgaagcatcg cttttacaaa tgtttttata aatccaattg caaacagaat
     4321 aagtccaatc aagccggtgt tcatccatat agcaagccat gagttgtgaa cgccaccctg
     4381 atgacctaaa gcacttagcc aatgtctgtt ttcatagaag taccactcat cataagcaaa
     4441 ccctcgccct aagaaaaact gtgattgaat ttgtttccat gcagcacgcc acgcaatcaa
     4501 cctacctgag ccatcttcaa tagcttctgc acgtaaataa tgtccgagac ccaatgcttc
     4561 tacaatagcc actatattct gattgattaa ctcataagca acaagtgcaa caaccagaat
     4621 tgcaaatcct gcaaggtagc ccatctcata gaaacgagca aacaataaaa atataactat
     4681 tgacatcatg gtattccgag aaactgcaag cagtgtactc aaaatgatta gtccgtaaac
     4741 aatgcgtgtt tcattctgat caagaagtcc gggaaatttt ttcaaggaaa taaatacaag
     4801 cataaacagc agggtgcatg caataccaat accattagga ttcccataaa cgccacagaa
     4861 acgaccaaca agaaaaactg tattaggaaa caatggaatc atgactatgc ctattaaaag
     4921 aatacaacaa aaaagcatga tgacttcttt tataaaactc tcacccaact cacgaaaagc
     4981 tttaatgaaa taggcaggaa ctacggtaaa cagcaatata taacttaatg ttttcatggc
     5041 actattcata aaatttacag atggtgcttg cattaacatt gctgcactcc atataaaaaa
     5101 caaaatgaat ggtttaaaat aatcgtttgt acctgtgaag ttttttctat cgaaaaaata
     5161 aaacaaagac agcaatacga tgtaaatatt ctttgcctga gctgcatata atgcttctga
     5221 ttgacgacta tcgctcatca caagtagaaa aaacaagcct aatatcaact ctttgtacat
     5281 gccctttaat ttgagcatta ctacactcaa cgctactgca gcaacaccaa gtgtttcatt
     5341 taccatacca cagaagaccc atgcaaaaca catgatcaca aactgtgcat tatttcggat
     5401 aaactgaagc atttggtggt ttagagctac aaatttaata tttatgtttt ctgttcagcc
     5461 atttgttgaa agagttcaag atgttgctgt gcaataaccg gccaggtaaa tttctctttt
     5521 actcttaggt atgactgctc tgccaaacgt ttttttgctt gttcgttcaa aacaaaatac
     5581 atagaaattg catctttaag agatttcaca tcagacttct caatgatata gccatttaaa
     5641 gaatcaacca taacggtcgt tgcgcctgta tctgtaacaa ttataggcaa tgaataactc
     5701 atagcttcta atacaactgt aggcatacct tcaaacaatg ttggtaaaac aaaaatgtca
     5761 cattctttat aaagactgtt taacttatca tcatcggcaa agccatgaaa aactacatta
     5821 ggctctttaa ccgtagtgct gaaatgttca aacaagggac ctttgccgac caaatgaaac
     5881 tcaagttcat ttttatagcc gccattattt aattgggcag ctgcatccat caatacatga
     5941 atacctttat tagcagcaaa ccttccaaca aacaaaagtt ttataggcct tttatcaaaa
     6001 actgatcgct caaatttttg agcaggcaga tttattgcat tcggcaacac aacaatttta
     6061 ttatgatgac ttttaacatt actttttaat agttgtgtta aacggccacc gagtgaaaca
     6121 acttttgcag cttttgaaaa aagatatcca aaaacaaatt taaatggtat agcttttaat
     6181 ctatctgaga aagaaagtgc ctgataaaat tccaatccat gaggatttag tataacacgg
     6241 cttccaattt tatctatatt atgccaaacg gccattcctt gtgcataaat tacagcatca
     6301 ggaaactgtt ttaatgcttc atatacttgc tttgaatagt aatactcatt caacagataa
     6361 ttactgctat gtttgttgcg cttgatgcca atttcagtaa tattcaatga tgcatcaaag
     6421 acattctgtt catgatgctg atgaataact gcaattttaa ttttttttgt tcttgccagt
     6481 tcttcaacca acaaacgtgt atgtttttga attccaccaa caagttgcgg atatacacca
     6541 tcagtacaaa aaaccacctg aacaggttga ctcattgcac caatgtttta attccttctt
     6601 caattccaat tttaggagtc catccaagtg atttcaattt agaaacatct gcaagcaaat
     6661 gttgtctttc tactttacga actcttgact gatccacttc aatttctatt ttttcattta
     6721 ttgctttaga gaaagcatca acaatttctg taaccgaata ctcaatacca cgaccgagat
     6781 tataggtatg aatacctcct tcaacatggg taagtaatgt ttctacagct gcagccatat
     6841 cgtaggtgtg aataaaatca cgtttgggag taaggttccc taatttaatc actctctttc
     6901 cctcaagaac ctgtttttga atttccggta tcaggtgtgg attggtttca ttcgggccaa
     6961 aggcattaaa aaacctgcaa acaataacag ggatatcagt ctctaaaaaa aactcattgc
     7021 ataaatgctc gcctgttagt tttgataatc cataaatatc cattggacct gtggcaaaac
     7081 tttcactcac agctaagtcg taaattggat aaacggcagc tgtagatgca aaaaacactt
     7141 tctccggtgt atatttttgt gctgccttta ggaggtgcat tgttcctctg atgttgatat
     7201 tactgctctc gtaaggatgt tgattacaat agggaataaa atgtactgct gccagatgaa
     7261 taatatactg aggtctgatt ttacctatga tttcatcaag acgctgttca tcaagaatat
     7321 cagcattaaa aaaatgactg tcatcaatat tgataaactg tctgttgcca aaagatagat
     7381 tgtcaattac aaaaatctca tgtttttgtt tctgaaggtg tgcaattacg gctgagccaa
     7441 taaaccctgc accgcctgtt actaatatcc gtttcataaa aaatggtggt tataaatttc
     7501 tatgtttcta ataaaggctt gttgtacgaa tgttttgtaa aaaagtttaa gtcattaatt
     7561 gtattaaatt ggtataactt gcattgtgcg atttttttgt aaagtggtca tttaccagtt
     7621 gaagcagcct gtttctttcg gaaatattca accgattcat ctgcaacgga gattcgtcca
     7681 acacccaacc gcaatcattt tgctttacaa atgaagtata atctccaata ccttcagaga
     7741 taattacagg taagcctgca gataaatatt cggcaaactt agtgggtgag gctacctgat
     7801 tggtaacact tttctctcta attaaaattc caatatcaca tgatttcaat attcctgaca
     7861 cttcactgtg ttttacaaaa gtctgaaaaa ttttatcagg aaactctgct ttcagctgat
     7921 ctacattttt atccttttcg gagagaaaca gcactttaat atttttatta ttattgataa
     7981 ttttctgcaa aaatccctgt aataaatcaa atgactgcca ccctgctgtt gaaccggaat
     8041 aaaccagaat gatatcatct gcattgaatt tcattttttt tcttgcattt tctatttcct
     8101 cctgttttaa aatttcagga cgaaaacttg tgtttagtgt acatggaata acaacatgct
     8161 tgttttcact gtagccataa cgttcattcc aatattttac caattcttct gaaacggcta
     8221 tccgaaaatc ggcatttagt acaactgact tttcccatgt atgaatacta tttttccatt
     8281 cctgcaacgg cactacctga tattcattcc attcagcagc gatggcacca cgaccatcaa
     8341 aacatacttt tagttttaag atagacttta aacgcaatgc tatccgtgca gccaggacgt
     8401 ttcttgcaat tacacttcgc tgatttagaa atagtaaaac aaaaaacagc atcagcgtat
     8461 taaaaccaaa acttgtcatt cgcggaagca ttggcaagac aattgccgag ggcatttcac
     8521 gtttaatttt ctgtctatta tgactgaaat tgtgcagtga aataaatgac actaatctaa
     8581 tttgtgcatt gtggctttca ttcaaaaaat tacatacatc gactacctga ctcgaataaa
     8641 caccagaggg ctgatcggca taagtaagat aaatcatatt ctgactcttt agggcaaaga
     8701 aacatgatta agcatgcatg tttcctctgt acttaatgta cttttcaaca aacagtatta
     8761 acacaattat cttagcgatc ccgccttaga ggataattgc cgacccactg tctttttgtg
     8821 actatgtgat gaagtcttaa aaacccgttg aaaacgcagt gtaaaacatc tgatttgtaa
     8881 ttataggaat cttattttcg tcccggaata actataacct gttacaaatg aattggaaat
     8941 tttggaaaag cagcggacgt aaaagtaaaa cacgtgagtg gattgatgca ttggtttttg
     9001 ccgttgtagc tgcaactata ataagaactt ttttaattga agcttttact ataccaacac
     9061 cttcaatgga aaaatcgttg ctcgttggcg atttcctttt tgtgagtaaa gtaagttatg
     9121 gtgcaagaac acctatgact ccattatcgt ttccatttgt acatcaggaa ttgccgataa
     9181 ttggcggaaa gtcgtacagt gatgcaatta aatggggcta ccatcgcctg ccgggatttg
     9241 gcaatatcaa aaacaatgat gttgttgttt ttaattaccc gggaaatgta ttctaccctg
     9301 atgatgattt aaaaagacct attgataaaa agacacatta catcaagaga tgtgtcggca
     9361 ttcccggtga cagtttaaaa atcattgatg gacttttgta tgtcaacaac aagccggttc
     9421 cacttcccga acatggacaa atgacctaca ccgtaattac tgatggcaac actatcaatc
     9481 caaaaacatt agagaaatac aatatttacg aaggtgcaaa tcttggaaat ggaatctatc
     9541 agtttacatt gactaaggaa attgcagatg aattaaagaa attgcctttc ataaaaagtg
     9601 ttgaactata taatcaactt cccggtgcgc caagtcagtt tcaaaaatta tttccatttg
     9661 attcacgtta caactggaat attgacaact atggaccgat ttggattccc aaagccggaa
     9721 acaccattac tttgaccgaa gcaaaccttc ctttttatca gcgctgcatt gttgaattcg
     9781 aaaataactc tttagtcata aaaggcaatc aatttatcat taacggaaca cctactacta
     9841 cttacacatt caaacaagat tactattgga tgatgggcga caacagacat aacagtgcgg
     9901 attcacgatt ttggggattt gttccggaag atcatattgt aggtaaggct gtttttatct
     9961 ggctaagctg gaacagcaat ggcactttct tcgataaaat aagatggggt cgcttattta
    10021 attttataca ttaatgtcca ttgctgtatt ttcaacagca tattttcctt cactggaata
    10081 cattagacat attcttcagt tcgatgagat aattatcgaa actaaggagc attttccgca
    10141 tcagaccaac aggaatcgct gtcacatcct tggccctaat ggaatacaaa cgcttactgt
    10201 tcctataaaa caaaatcata taaaaacacc aacaggtaat attgaaattt gtagcggcaa
    10261 atggaagcag caacattggc gtagcctaac tactgcatat aacagaagtg ccttgtttga
    10321 attttataaa gacgaattag aatctattat atttgacaac catcgtctct taattgaact
    10381 aaacacaaaa attttatcgt tcattttgaa agccaaaaag aaaacgatag caataaattt
    10441 taccacatct tttcaggcga gctacaaaaa tgacttccgc tatatctctg atgcaaaatc
    10501 acaaaaacaa atttctgatt taaacattcc ctcctatcct caggtttttt cagaaaaaat
    10561 tgggtttgtt tctaacctga gtgccattga cgcattgttt aacgggtcac tataattcca
    10621 cataaaaaaa cctactgtac tattatttac ttttgttgtt tctccaataa acaatagtga
    10681 ataagaaaaa aattattaac gacccggttc atggttttat aaccattccg tccgaattta
    10741 tattcgacct gatagaacat ccatactttc aaaggttgag gcgaatcagg caattgggat
    10801 tggcacaata tgtctatccg ggcgcagtac acacgcgatt tcatcatgct attggtgcaa
    10861 tgcatataac acaaattgca attgaaacat taagaaacaa aggtcatgaa atttctgcta
    10921 ctgaagaaga agcagtgagt gcagctatct tacttcatga cattgggcat ggaccatttt
    10981 cccatgcttt agagcacaca cttgttgcgc atttaaagca cgaagaaatt tccatgatga
    11041 ttatggagaa aatcaatgaa tcaatgggtg gtgcattgaa tatggcaatt gaaattttta
    11101 aaaatgttta tcccaaaaaa tttctacacc agttagtcag cagccagttg gatatggacc
    11161 gattggatta cttagcacgt gatagttttt ttacaggtgt ttctgaaggt attatcagca
    11221 gtgagagaat tttaaaaatg ctaaacgtat ttgatgacaa gctggttatt gatgcaaaag
    11281 gaatttattc agtagaaaaa tttattgttg ctaggaggct aatgtattgg caggtttatt
    11341 tgcataaaac agttcttgct gcagaaaacc aactcatcaa cactttaaaa agagcacgtt
    11401 atttgactgc aaaaggtcat gatttattcg gaacaaaatc atttaaaata tttcttaaaa
    11461 ataattttac ttcaaaagac tttaagcaac aaccacattt aatagatgag ttctgtctgc
    11521 ttgatgactt cgacatattt accagcatta aagaatggat gacccacgat gattttattt
    11581 tatctcattt atgtaaaggg ttgattgaca gaaatctgtt taaaattaaa ctacaggaaa
    11641 agtcatttac gcaacaagaa acagaaaatt atttaaagaa agctgtaaaa gcttataaca
    11701 ttacagagga agaagcatca agtctagctt ttacaggaaa aatcgaaaac agtgcatata
    11761 caaaaagtca ggaaaacata aacattcttt tcaaggataa tgtaattaaa gatattgctg
    11821 atgcttcgga caacttgagt atttctgccc taagcaagcc tgttgtgaaa cattatctat
    11881 gtgttgactc gggatgcctt tagccggtgc aatttcagtt ttcaggaaaa atcagttgag
    11941 gttgtaatga ttttagttga aatgatttcc gatggtaagg agacaggcca aatttcttaa
    12001 ttgcttcaac atgaattgct gtcgcatacg ctttatttct atcccaacaa taaacagggt
    12061 gtttatgatg taactgaagc atatattcat cgcgatgtgt tttagcaaga acagatgcag
    12121 cagcaatgga agcaaacaaa ccatcccctt ttacaataca atgatggggt actgctttat
    12181 aatttttaaa cctgttgcca tcgatgagta atctttcagg aaatatttcc aactttgaga
    12241 ttgcattatg cattgcttta tatgtagcct gcaaaatatt aatcttatca atttcggaat
    12301 gtgtacagga tgccactgcc caggccaaag cttctttttc aataactaaa cgcaacttat
    12361 ctctttgtgc agcagtaagc ttttttgagt cgttcaattg agcgttacta aaatcacgag
    12421 gaagaattac agcagcagca aatacagggc ctgccagaca acctcttccg gcctcgtcta
    12481 tgccggcttc tacatcatca gaaaaatatg atttaagcat ctctgagcaa aaataaatgc
    12541 attaatttaa ttactcatac aactttaact aaaatggtgc atatttaagt atatttgtgg
    12601 cgttcctcaa ttaaaaatgc caatacaatt cgtttatccg ttttttcttt ggtttctggc
    12661 agcgctttct attcctatca ttatacacct gtttaatttc aggagattca aaaaaattga
    12721 attcaccaat gtcaggtttc tgaaagaaat aaaagaacaa acgcaatcgc attctaaatt
    12781 aaagcacttg ctggtattgc taatgcggct tatggcatta gcctttctga ttctggcttt
    12841 tgcacaacct tttgttccaa ccaagtcaac agagtcatca gggcagcaaa tcgtcagcct
    12901 ttatattgat aattctttca gcatgaatct ttccggcaag gaaggttcat tacttgatga
    12961 agcaaaagat aaatgcagaa ccattgctac atcactcccg tcatcaacaa agtttcagct
    13021 acttacaaat gaatttaaag gtgaacagca acgactcatt gaccgtgatg aattgcttga
    13081 gctaacagat cacatcaaaa taaatccgca aagtagaaac atcaatgaag tattaattcg
    13141 tcagactgat gcattaaaaa aatcaaactc tgacaatcgt tcatcattta ttatttctga
    13201 ctttcaaaag agcttctttg atatgcaacc gggagttaat gatagtctct taaaattaaa
    13261 tctactaaaa acagatgccg aagaagtaaa aaatattgca atagattcat gctggctaag
    13321 tgctccggta attcagctga atactccatc tgaactcaat gttaaagtca gaaactacgg
    13381 taacgaagat gccgaagtac ctgttagatt ttatatcaac aactcacaaa aaacagtggc
    13441 atctgcaaaa attaatgcag gaatggacgc taccgtaaaa atgaacttca ccataataca
    13501 gcctggctgg caattggcaa aagtagaaat tgatgacaac ccgattgctt ttgacgatac
    13561 ctattatctt tcttttcgaa ttgcagataa aattcctgtt tatcatattg gaagcagcaa
    13621 gaatattttc attgacaaac tcttagcgaa taatagttta atagattata ctttcagcac
    13681 tcaaaatgca gttgattatg ccatgctgaa aaagtgcaat gcagtaatcc taacagacct
    13741 taaagacata tcatccggaa tgagtgatga aattgcacgt tttgttgatt tagggggcac
    13801 attaattatt tttcccgatt ctaatatcac acctaatggt tatgaaaatc tttttggtaa
    13861 actgaatgta gaaaattaca gtaccctaaa caccaacagc atcagtgtta gtaaacttga
    13921 cttagccaat ccgttgtttc acgatatgtt tgagaagacc gatgaaagga tgagtttacc
    13981 tacagtaaat cagagttatg aaacagtatc ttcactcagg tcagggaaaa acatagtaat
    14041 gcaattgcaa ggcggtcatt cttttttaaa ttattaccat agaaaatccg gtagtgttta
    14101 tcagtttact tcagcagcac aacctgcggc aggtactttt gttactcatg cattatttgc
    14161 acctgtactt ttcaaaacca tattgttaag tgccccatct tctcagccta ctctgtttat
    14221 cggagaaaac agtgacatta aaataccctc atcaatacaa acaaacgatg tgttacattt
    14281 aattaacagc gatacaaaaa cggatattat tcctgaattt aaaactacaa atgatggaac
    14341 actaatcaat ctaaccggta tagaacaggc aggcttctat caattgaaaa atttaaacga
    14401 cagcatactt tcaatcattg cattaaatta caatagaaaa gaatctgatc tgaaagcctt
    14461 cagtgatgag gaattaaaaa gtatgtatgg taacggcaaa ttcaaatcag tagtagtagt
    14521 taatcctact ggaagcggcc tcacaaaatt tattaaacaa tactcatcag gcatttcact
    14581 ctggaaatat tgtatattga caacattgct atttttaata gcagagacat tattacttcg
    14641 cagtgacaga aaaatggtaa cactaaaaaa ttaaaaccat ggatataatc ttaaaatcgg
    14701 taaggattat caacccggga accacacatc atcaaaagac acgtgatatt attattgtca
    14761 acggaaaaat taagagtata actacaaacg caggtaatga ggaaggaata aaagagcttg
    14821 acctgaaggg ttgttatgtg atgcccggtt ggtttgatat gagagcaaat ttcagagagc
    14881 ccggtgaaga gtataaagaa gatcttggca gcgggataaa agctgcagcg gcaggtggtt
    14941 ttacagaggt attattaatg ccttcaacat tatatccgat acaatcaaaa agtgatactg
    15001 aatttatttt aaacaaatgc cgaaacagtt tggttaatgt tcatgttgca ggatgcctga
    15061 ctactgacag aaaaggtatt gacatggcag aactatatga gatgtatgtt tccggagcca
    15121 ttgcctttac tgatgataaa aaagttaacg accccggaat gcttgtaaga gctttacaat
    15181 acagcaacac atttggcgca agaattattt cgtatgctga cgaaccatct ttaagtaaca
    15241 actatctgat aaatgaaagc cccagcagtg tgagaactgg aatgaaaggt tgtccggcag
    15301 tggctgagga aatcgggtta aaccgcatta tcaggattgc tgaatataca ggaataccgg
    15361 ttcatattag cggaataagt acagcaggaa gtgtggtaca aattcgcaat gcaaagtcaa
    15421 aaggaattaa aattacagct gaagtaaatg cacacaattt aatgcttgat gaaactttag
    15481 tagaatcatt tgacagtaaa tacaaagtaa aacctgtatt gcgaagcaga acagatatac
    15541 aggcattaaa aaaaggaatt accgatggtg tgattgattg tgtatgcagt gaccattctc
    15601 cgcaggacat tgaggtaaaa aattgcgaat atgattatgc ggcacatggc atttcatcca
    15661 ttgaaacaac atttgctaca ttaagtacag cctttaaagg tcagcttgat gctgaaattt
    15721 tatttaatat tttttcaggc aatccacgta aaattcttgg tttggaaatg ccaagcatca
    15781 atgaagctga aacagcaaat atgacagtat atcatcctga caaaacctat attttttcag
    15841 agaaggaaat gttttcgaaa tcgaataaca ccccatttca cggtacagaa ttcataggta
    15901 aagttattgg tgtcgtcaac aacaataagt taagtggctt ttaggaattc tgaaagcgat
    15961 tgattgccgc aaaaaaggaa ttttttttca aacgtgaaag cggagcagat ttaccattct
    16021 tcaaaataat cttttcctga ttttttaaat actcaattac ataagataag ttcacgataa
    16081 aattttttgc tgatctgaaa aacattatat ccggaaccaa tttttctatt tcagccagtg
    16141 ttattgtgct gataaaatca tctccattga tcaaaaagac ctgattaaac tttccactac
    16201 tctctacaca taatatctga ctgacttcaa caaatctata accggtaaga acaggaatag
    16261 ccaattgcat agcttcaggc tttttttgaa tattctgcat cagcaaaccc aagtaatcca
    16321 ttgtttgttt ggatgcaatt ttctctttta actttttgac agatagctta agttcttctt
    16381 cgccaaatgg cttaagcaga aaatctactg cagaaacttt aattgcttta tatgcaaaat
    16441 cctgataggt tgtggtaaaa atgacttcaa aatcaatttc aacaaatcgg tttaacagat
    16501 caaaccccgt aaaaggctcc atcatgatgt caagaaaaat aaactgaggt ttcatttttc
    16561 tgattgccgc ttcagcctgt ggtatggtaa aacagatatc taaaacagta atttcggggc
    16621 atgctgtttc caccatcttc ttcagacttg ctgcctgcat ggaatcatct tcaataataa
    16681 gcgaggttaa cttcacaaca atcaaaaaga ttaaactgca aaaataaaaa taaggcaatt
    16741 gaatgtttta ttctcaacaa taaccttaac aacatttcct atttgtaaac gaggaaacaa
    16801 ttcaaatttg catttttgca aatctgaata gcttaagcat ggttaaacca aaaaaatatc
    16861 ttggtcagca ttttttaaca gacaagaata ttgccaatac tattgttgac tcactcactt
    16921 tatctcaata tagtcaggtt attgaaattg gtccggggat gggagtgttg acggaagagt
    16981 tagttagtcg ttttgccgga aagttaacct tgattgaaat agatgaggaa tcggcagcgt
    17041 atatcaaaca acactttgat agtagcaagt tccgattgtt acaagaagac tttttacacg
    17101 ctgacttgaa taatattatt tctgtcccaa ctgctattat tggaaacttt ccatataaca
    17161 tttcaacaca aattcttttt aaggtatatg agcatagaaa catgatacct gaagttgtgg
    17221 gtatgtttca aaaggaagtt gctgaacgta tttgttccgc tactggaaaa aaagcttatg
    17281 gaatacaaag tgtattactt caatcttgtt atgattgcga gtatttgttt acagtagagc
    17341 caaatgtttt caatcctcca ccaaaggtta aatcaggtgt aatcaggctt aaactaaaca
    17401 gtactaaaaa aattgttagc aaagaatcgg actttagaag agtggtaaaa gccgcattta
    17461 atcaaaggcg taaaatttta aaaaatgctt taaatcaatt aacttcagtt atggctgaaa
    17521 agggtgaaat gccttttgga agtaaaagag ccgaagaact acattggaca caatttgacg
    17581 aactgacaca atggttttac aaataaatta agaatagaaa cgacttaata tttatagcgg
    17641 agtcataaaa ataagagggg cgatgcaaat gcatcgcccc tcttatattg tttaaccaaa
    17701 aaactactcc actaccactc tcacctgatt gttgctgcca ttggtttgta cat
//
LOCUS       DAOQNP010000066        17747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1121_length_17747_cov_7.639894, whole genome shotgun sequence.
ACCESSION   DAOQNP010000066 DAOQNP010000000
VERSION     DAOQNP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17747
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_17747_cov_7.639894"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..696
                     /gene="rsmG"
                     /locus_tag="PKY09_09405"
     CDS             61..696
                     /gene="rsmG"
                     /locus_tag="PKY09_09405"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNB13114.1"
                     /translation="MSSGVSLLQRYFPELPLEKQEKFILFAELFKAWNEKINLVSRKD
                     IDQFYLHHLLHSLVIAKFIDFGSGTKVMDIGTGGGLPGLPLAIYFHETQFYLVDSIRK
                     KTEAVKAMADQLELSNVTVINQRAEEVAEKVDFVVSRATAPLSDLVNWSRGKINAKSK
                     ASIPNGLICLKGGDLTEELKPFENKLFTENVTAFIQEEFYKEKKIIYLPLV"
     gene            763..924
                     /gene="rpmH"
                     /locus_tag="PKY09_09410"
     CDS             763..924
                     /gene="rpmH"
                     /locus_tag="PKY09_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNB13115.1"
                     /translation="MVRTYQPSKRKRKNKHGFRERMATANGRRVLASRRAKGRKKLTV
                     SSESKVRGK"
     gene            1011..1559
                     /locus_tag="PKY09_09415"
     CDS             1011..1559
                     /locus_tag="PKY09_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13116.1"
                     /translation="MNIKSGLFYLSIILLFAACQKDYNDPGTGPSQYYGTRSGVSIRA
                     ILLAQMPATDPSGNNWDVADSVDTAGRADIFYRFKDIDSIDYPEFVQSFHFDNVNPDS
                     LPLPYFLTTPYPLRAIGSNVQFDIYDFEKSISSPAIDSTLMKSFNFSVVAGDSLFENP
                     YPDSLVLAEDGYVVLLYLNWIK"
     gene            complement(1647..2600)
                     /locus_tag="PKY09_09420"
     CDS             complement(1647..2600)
                     /locus_tag="PKY09_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13117.1"
                     /translation="MKKLFTLLTALALGSLTSNAQLIYDNGSIVNQPGAGAGGADVSS
                     LHDGLSSFGIGHAVSSGYRVADDFIIPAGQTWTIDSIVFLAYQTGSGNTSTMTEVNCA
                     IYDGAPDVGILVFGDQSTNMMQNTYFSNIYRTTETTFTNTDRPVMRNVIVPPSAWSLS
                     AGTYWLDWQTNGTLTSGPWAPPLTNANTITGNGMQFDATNSVWGPCNDGGTLTQQGFP
                     FEIYGTITTSINNVEAEEILTVMPNPSKGTFRINAAFTGKTNLEINVTDLMGKTVYSN
                     MLNDISVVSKTVELKNAAKGVYVLKLNSDSGKTMTKKIVVE"
     gene            complement(2720..3715)
                     /locus_tag="PKY09_09425"
     CDS             complement(2720..3715)
                     /locus_tag="PKY09_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNB13118.1"
                     /translation="MKIVIPVAGIGTKLRPHTHTQPKTLVPVAGHPILAHIIDYFVQS
                     GYDEFVFIIGYMGEKIEEFVKSKYPSLKAAFVVQEPREGTGHSVWFARNLVHAEDEML
                     IVLGDTIIDFDINVFKQSEQSLLGVKKVDDPRNFGVVELNDDGFVSKLHEKPVIPKSN
                     LALVGIYFIKQAGHLFEALNYIIENNLKTRDEYHLTDAMMRMIEDGEKIKVFHVGNWF
                     DCGNKDSLLETNAILLDRIAETNPKPQKFPNTIIIPPVSIAYNCDISDSIIGPHVSIG
                     EHTIVKYSIIKDSIIGSYSQLESAVLHRSVIGSDAFLKGLSQSLNIGDSTEIDFS"
     gene            3827..>4236
                     /locus_tag="PKY09_09430"
     CDS             3827..>4236
                     /locus_tag="PKY09_09430"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190481.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HNB13119.1"
                     /translation="MLNKITELLGKDADKLLNHVCKTVPKETLHQPGNDFVDRIFIPS
                     NRSNQVMRSLQSLYNHGRLSNTGYMSILPVDQGIEHSAGASFAPNPMFFDPENIVKLA
                     IEGGCNAVASTYGVLASVSRKYAHKIPFIVKINH"
     assembly_gap    4237..4336
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4337..4735
                     /locus_tag="PKY09_09435"
     CDS             <4337..4735
                     /locus_tag="PKY09_09435"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066424.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HNB13120.1"
                     /translation="DLTAQANHLGVTIQADIIKQKLPTNNGGYTAINFGKTHKDVYTK
                     LSSDHPIDLCRYQVLNCYNGRMGLINSGGESKGATDMAEAVTTAVINKRAGGQGLISG
                     RKAFQKPFKDGVEMLNAIQDVYLSKEVTLA"
     gene            4797..5642
                     /gene="accD"
                     /locus_tag="PKY09_09440"
     CDS             4797..5642
                     /gene="accD"
                     /locus_tag="PKY09_09440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNB13121.1"
                     /translation="MTWFKRIKEGITTSTKEKKETPEGLWYKCPTCKSIVPSLEHEEN
                     KYVCPKCNYHDRIGSEEYFAILFDDNQFTEFNAALTSADPLGFVDTKKYTDRLRDTIS
                     KTKLKDAMRTAVGKVNGNNLVIACMDFQFIGGSMGSVMGEKIAGAIDYSIQHKMPLMI
                     INKSGGARMMEAAFSLMQMAKTSAKLTRLADAGLPYISLLTDPTTGGVTASFAMLGDL
                     NIGEPGALIGFAGPRVVKETIGKDLPKGFQTSEFVLEHGFLDKIVDRRQLKDTIGQLL
                     SMFKN"
     gene            5758..6633
                     /gene="ygiD"
                     /locus_tag="PKY09_09445"
     CDS             5758..6633
                     /gene="ygiD"
                     /locus_tag="PKY09_09445"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNB13122.1"
                     /translation="MYRREFLSSIAGIGAIGTLSSFKNITDDLSNSDKMPVLFIGHGS
                     PMNGIENNEFSNQWKKFGQSLPIPKAIVIVSAHWLTKGTWITAMKSPKTIHDFGGFPK
                     ELYDVQYNAPGSPELAQETKSIIKSVNAELDHDWGLDHGAWTVTRRMYPDANIPVLQI
                     SIDINKPAAFHYQLGKELASLRTKGVLVIGSGNMVHNLGKIAWDKIDEVGFGYDWAVE
                     MHQLFKSKITDNNHQALINYTSLSKSALLAVPTPDHYFPLMYILGLQQKNEKAEFFND
                     KLVAGSINMTSVKFG"
     gene            complement(6650..7627)
                     /locus_tag="PKY09_09450"
     CDS             complement(6650..7627)
                     /locus_tag="PKY09_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNB13123.1"
                     /translation="MLHKSFFTAIVFLFLSTSLFSQKRIKIPVLDYGSVDEFVLKLKP
                     EQCVNIDTLAAVISREFDRPHFRFRAAFRWVTENVAYDIKNKRHPQKSNSNANEVFKN
                     KTALAEGYANILNAICAKMNITCLSVNGYLRQNEFPGTKIKKPNHIWNTVKLYDKWYL
                     MDVMMASGYFENGDKIFTKRFTERYFALHPRQVILTHLPLNGNRQYLDTIVDKNTFLR
                     YPKVYDFFFDNGIYALRPADGVIPVFKGQKKRFYFKSMDSLAIGKIEIRDGKKSEKIQ
                     NTFTQVDSLLYFDYVFESNKKRTLDVFVDGLPLCTYSIVPDKKKKRLED"
     gene            complement(7691..8764)
                     /locus_tag="PKY09_09455"
     CDS             complement(7691..8764)
                     /locus_tag="PKY09_09455"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNB13124.1"
                     /translation="MALSFFLAAYILILEFKRKERNGLLKSFTVTTIKGKPASTSELF
                     WSGAVGFLVGFKLLDAILNYSDLVNDPQGFLLSSRGNVIGGILLGAYSVWSKYKEKEK
                     QKLAVPQTVTEVGYPHQFVMNITIAAAVAGLIGAKIFHNLENIDDFLKDPVDALISFS
                     GLTFFGGLICGALAVIWYSSKKLNVPPLVMCDVAAPALMLTYGTGRLGCHFSGDGDWG
                     IVNTLAKPSWFPFPDWMWSYNYPHNVIGEGVPIEGCVGKHCFELIPPVFPTPLYEAIA
                     CILLFFILWKLRTRIVTPGVLFFVYLLFTGVERLAIEQIRVNTKYHIFGKAITQAELI
                     AVACIIVSIVGITLLKRKNSGSS"
     gene            complement(8858..10183)
                     /gene="mtaB"
                     /locus_tag="PKY09_09460"
     CDS             complement(8858..10183)
                     /gene="mtaB"
                     /locus_tag="PKY09_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNB13125.1"
                     /translation="MKSTVAFHTLGCKLNFAETSTIGRQLQDAGYKKVSFSDAADIYV
                     INTCSVTENADRECRQHVNRALLSNPEGCVIIIGCYAQLKPDAIAAIPGVSLVLGASE
                     KFNIPHYLQKLKKKSEAQVFSCDIAEHHQFTSSYSEGDRTRVFLKVQDGCDYGCTFCT
                     IPMARGASRSNTVENVIENVKQIVNRGAKEIVLTGVNLGDFGKLPGEKKHTTEFFQLV
                     SALDALDADIRFRISSIEPNLLTDEIIELVSQSKKFMPHFHIPLQSGSDKILKLMRRR
                     YLTSHYTTRINKIKALMPDACIGVDVITGFPGELEEDFLQTYQYLNEADISYLHVFTY
                     SERANTPAIEMKPVVPIAERKRRTNMLRILSEKKLGIFYRSQQGRTAKVLFEHEDKNG
                     IMSGYSENYVRVTTPFNNQMVNRVTECIIGATDNDSHEVNCTILESIPA"
     gene            10297..12042
                     /locus_tag="PKY09_09465"
     CDS             10297..12042
                     /locus_tag="PKY09_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB13126.1"
                     /translation="MSTSGTAFNWKVLNRIMFYVRPFRFLFVATVVITVLLAVLAPLR
                     PWLTQQALDIDIAKHDIKGLTRTVAFMFGVLLLQSLLQFVYTTLTNRLGQEVILSLRN
                     NLFKKVSAFGLAYFDKTPLGITITRLVSDMETIADIFSDGLILIISDMLQVVVIIAVM
                     FYIHPLLAAISLSTIPVLLLATRVFQKNIRKTFNDVRIQVAKLNEFVQEHLVAMRIVQ
                     LFNREEEELNKFHDINAKHRDANIRSIWYYSLFFPVVEILSAVSIGLIVWYGGLNVLQ
                     SSISLGLLVAFIQYINQLFRPIRELADKFNTLQMGMVSSERVFKLLDEPFTEVNSGQK
                     SADKIIGKIEFRDVWFAYKNEEWILRGLSFTIPAGQSVAIVGATGAGKTTIISLINRL
                     YEIQKGRILIDDIDIREYDLYSLRNAIGVVLQDVFLFSDSIKNNITLYKEQDDIVIEA
                     LLKQTGAWHFVEKLPGQLHYNPGERGGLISTGQRQLLSFARVLAHHPKLIILDEATSS
                     VDSKLEQLLVNATEVLTKNRTSIIIAHRLSTIEKADRIIVLQKGSIVEDGNHQILLQK
                     KGVYAHLHEVQFMQP"
     gene            12094..12714
                     /locus_tag="PKY09_09470"
     CDS             12094..12714
                     /locus_tag="PKY09_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13127.1"
                     /translation="MKIFFSLFLTLCFAAGTTFGQDAASTDTIHHNHFNDGVYLSYED
                     LKSNRPTIKKEQLIKSYYDSSAFSISKWANTQNLYYATSAGDKQKVNRDSLWGYVENG
                     IPYIFLNDRFHKFSTSGAVSVFRESYPNLKAGLAPVVTEAKYTSQINLFILANGKIDE
                     FTPENVALAIEDNKALYDEFMALKSWKQKRKKMYRFVERYNDSAMW"
     gene            12736..14226
                     /locus_tag="PKY09_09475"
     CDS             12736..14226
                     /locus_tag="PKY09_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13128.1"
                     /translation="MKKIKLLFLTVFIFVVAIVPQGCKQKVAEVNKAGFVSIKDQKFY
                     LNGKPFFPLAVNYRVALQTDGQQLWPSAYKGYDEGNRYRFTNKDSSLEELKNDFQLIA
                     DMGFNSIRIVGVGEQYSVTKNSDELYIKADIGNERDTMFLFNSQETYNLYYNALNEMM
                     LAANLAGLRVVLLARLFHEVPATEKHWGRIMKHFQLDTMLMAYDFFNEPLYFDSLERN
                     KTEPYHITKAWNKLAKENAPHQLITIGLTGIREVFEWDPNTLNVDFISYHPYEYEPEQ
                     VRNEIYWYGKYVRKPWIIGETSVPADNDSVPYSVQKAFAEATFKQTCNCGGIGYTWWQ
                     YKDVDWFDFHSNFMGIINRKGTTRTSTNKEVIGTPKPITEFFKIAKPFMDASQCLKLE
                     NYYNYSNANVCCLKGKLVGSSGEPIEGGVVMAWSKDWANSYHTVTKQDGTFELLGSFP
                     FYHWIASATLFTMVRGDVLPDSSDNKNSKILTLDLGSLNLKKLNLE"
     gene            14370..15746
                     /locus_tag="PKY09_09480"
     CDS             14370..15746
                     /locus_tag="PKY09_09480"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNB13129.1"
                     /translation="MNHYNFRTFAWDLGINNNAIYDYAHFRWNDCMILQPQFKNVLAD
                     HFTLLPILVSPLYWFFGSYTMLLFQITAILFGGVGIYKYFLHKTSDSSLSLIAMIHFY
                     SVWGIFSALGFDYHDNVIGSMLVPWLFYSFEKEKWKTAAIYFLLICISKENMALWAVS
                     IVMTIYLLNYKSTRVRKISVLFGLFALVYFLFVVKVIIPSIANEGREYLHFNYKAIGN
                     NFSEAIQTLILRPQYAFSLLFENTTGWNEANFIKSELHFFVLLSGGYAILYKPQYLLM
                     LLPIYAQKLFNDDIGKWGLNYQYSIELVPILTLALYTFLYEINKRHKIYYLLLPCLIT
                     IVATASSMDHRISKWYQPEQVRFYDKKHYQTEYNVAEINERLKVIPKNAAVSASNCLV
                     PHLAFRDYIYQFPVTNNAEYIVLLKSENTYPLSHEEFENKISEYNTSIDWKKVVDDDN
                     LIIYKQNK"
     gene            complement(15755..16324)
                     /locus_tag="PKY09_09485"
     CDS             complement(15755..16324)
                     /locus_tag="PKY09_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13130.1"
                     /translation="MNSQRDMANNKLSIIKQLNITLIFSCLILVSCKDSKKQFTYLDT
                     ADSEIAFHSEKKVNNIIRFDNAHSGNYVYKLNKESPFSGTFYLGLSDVFPGNPKKALL
                     SAWILTEQINSALKFTLEIRDKDFNLKEWLGSNANDKMHVVDEWTYVEFEFDLLTNNR
                     NNSENFLRAYIYSDTNLNVYADDIEVRFY"
     gene            complement(16296..17648)
                     /locus_tag="PKY09_09490"
     CDS             complement(16296..17648)
                     /locus_tag="PKY09_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13131.1"
                     /translation="MKNKCIISKFSILLIFLVFCGYAFINKKWQKEEVLKYDSLIYYT
                     YLPATFIYHDLSLDFLSELPSTITNNIWYEKLSDNKKYLKMTSGVAILCSPFFLVAKN
                     IHTSDGQNKSGFGSKFQLSIVVAALFYLLIGLIYLRKTLLLFFDDIVTSLVLVIVSLG
                     TNLFYYTVFESGMSHVYSFSLVSVFLYYSIRWNHDDFNIKSLIILSIIGGLITLIRPL
                     NGIICLFPIFYNKAGFNLLQKMKVILSKPSRVFLAFFCFLLPFLPQLVYWKIHCDTYL
                     FYSYGQEQFFLDKPHVFEGLFGFRKGFFIYTPVMILLIPGLFISYKRMKNIFWAFSIV
                     TAIFLYLTFSWWCWWYGGSFSARTLVDFYALFALVMGCFFSFIIRKKFYAIVCILFLL
                     SSIGLNITQSFQLDKGFLHYDAMTFEAYKEIFLNLNEYKINENSLIHPNYEQSKRYGE
                     "
BASE COUNT         5860 a         3061 c         3085 g         5641 t
ORIGIN      
        1 ttcagagcac gcgaatttct gtatcagatt atgaaaaact cagaacatcg tccataagtg
       61 atgtcaagcg gagtttcatt attgcaacgc tactttccgg aattaccttt agaaaaacag
      121 gaaaaattta tcttgtttgc tgagttattt aaagcatgga atgagaaaat taatcttgtt
      181 tccagaaaag atattgatca attttatctg caccatctat tacattcact cgtcatagca
      241 aagtttattg attttggtag tggaaccaaa gtcatggaca ttggtaccgg aggcggattg
      301 ccgggcttac ctttagccat ttattttcac gaaacccaat tttatttggt tgattctatt
      361 cgtaaaaaga ctgaagctgt taaagctatg gccgatcaac tggaattatc taatgtaaca
      421 gtaatcaacc aacgtgcaga ggaagtagct gagaaagttg acttcgttgt ttcaagggca
      481 acagcaccac tttctgattt agtgaattgg agtaggggta aaattaatgc taagagcaag
      541 gcatcaatcc caaatggcct tatttgctta aaaggaggcg atttaaccga ggagctaaaa
      601 ccatttgaaa ataagctgtt tacagaaaat gttacagcgt ttatccagga agaattttat
      661 aaagaaaaaa aaataatcta cttgccttta gtataaaaga attaattata tctttgccgc
      721 ctctaaaaag aattttgcag cattaataaa aactgaatta ttatggtaag aacatatcaa
      781 ccatcaaagc gtaagagaaa aaataaacat ggttttcgtg aaagaatggc tacggctaac
      841 ggacgtaggg tattggcttc tcgcagagca aaaggtcgta aaaaactgac cgtttcaagt
      901 gaaagtaaag ttcgcggtaa ataaataact gcattataaa atatcaaaaa gctgcctttt
      961 ttaaggtggc ttttttattt tagcaccaat tatcaaagtt tctagttctc atgaatatca
     1021 aatcaggtct tttttatttg tcaataattc tactgtttgc tgcatgtcag aaagattata
     1081 atgaccccgg caccggtcct tctcaatatt atggaacacg cagcggtgtt tcaatacgtg
     1141 caattctttt agcacaaatg ccggcaacag atccatcggg aaacaattgg gatgtagcag
     1201 actctgttga cacagcaggt cgtgcagata ttttttatcg tttcaaagat attgactcta
     1261 ttgactatcc tgaatttgtt cagagttttc attttgacaa tgtaaatcct gacagcttgc
     1321 ctctgcctta ttttttgact acgccatatc ctttacgtgc aattggcagt aatgtacaat
     1381 ttgatattta tgattttgag aaaagtattt cgtcaccggc tatagattca accttaatga
     1441 aatcttttaa cttttctgtt gttgccggtg attcactttt tgaaaatcca tatccagata
     1501 gtttggtact ggcagaagat ggatatgtag ttttactata tttgaattgg ataaaataat
     1561 aaattgaaat taagtttaat ttctgttact taaaaacaaa gaaggcagcc cttttgaggc
     1621 tgccttcttt gctaaatcaa ctaaacttat tcaactacaa ttttcttagt cattgttttt
     1681 cctgaatcgc tgtttaattt taaaacataa acacctttag cagcattctt gagttcaact
     1741 gtttttgaaa caactgaaat atcattcagc atattggagt aaaccgtttt acccatcaaa
     1801 tctgtaacat taatttccag attcgtcttt cctgtaaaag ctgcattaat tctgaatgtc
     1861 ccttttgaag gattgggcat tactgtaaga atttcctctg cttcaacatt gttgatagag
     1921 gttgtaatag tgccataaat ttcaaacgga aaaccttgtt gtgtaagagt gcctccatca
     1981 ttacatggtc cccatacaga attggtagca tcaaactgca taccattacc tgttatagtg
     2041 ttggcattgg tcaatggagg tgcccatggt ccagatgtca aagttccatt tgtctgccaa
     2101 tctaaccagt aagttcctgc actaagactc catgctgatg gtggtacaat aacattgcgc
     2161 attaccggac gatcagtatt tgtaaatgtc gtttctgttg ttcgatatat gttagaaaaa
     2221 taggtgttct gcatcatatt ggtagattga tctccaaaaa ccaatattcc aacatccggt
     2281 gcaccatcat aaatagcaca gttaacttct gtcattgttg aagtattacc cgagcctgtt
     2341 tgataagcta agaacacaat ggaatcaatg gtccaggttt ggcctgcagg aatgataaaa
     2401 tcatctgcaa cgcggtaacc acttgaaaca gcatgtccaa taccaaatga actcaaccca
     2461 tcatgtaagg aagaaacatc ggctccaccc gccccagcac caggctgatt aacaattgaa
     2521 ccattgtcat atataagttg tgcattagat gtcaggcttc ctaatgctaa agccgttaat
     2581 agcgtaaata attttttcat attttattga ttttgaaatt tacaaatcaa aagtagatta
     2641 ttatttaaaa taattatcaa aacaacaatc tttttttaat aaaatataac cattaatttc
     2701 aataaatatg ttaataacat caactaaagt caatctccgt actgtcaccg atattcagac
     2761 tctgacttag tcctttcaag aatgcatcac tcccaataac agagcgatgt aaaacggcag
     2821 attctaactg cgaataagag ccaataattg aatctttaat gatagaatat tttacaatgg
     2881 tatgctcacc aatagaaaca tgcgggccaa taatggaatc ggagatatcg caattgtagg
     2941 caatactcac gggtggaatg atgatagtat ttgggaactt ctgtggttta ggatttgttt
     3001 ctgcaattct gtctaataaa attgcatttg tttcaagcag gctatccttg tttccacaat
     3061 caaaccaatt acccacatga aagactttaa ttttctcacc atcttcaatc attcgcatca
     3121 tggcatcagt cagatgatat tcatctcttg ttttcaggtt attttcaatg atgtaattca
     3181 atgcttcaaa caaatgccct gcttgtttaa taaaatagat tcctacaagt gcaaggtttg
     3241 actttggtat taccggtttt tcatgtaatt tggaaacaaa gccatcatca tttagttcaa
     3301 cgacaccaaa attgcgtgga tcatctactt tttttactcc taaaagtgat tgttcggatt
     3361 gcttaaaaac attaatatca aaatcaataa tagtatcacc caatacaatg agcatttcat
     3421 cttctgcatg aacaaggttt cgggcaaacc aaacagaatg gccggtgccc tcgcgtggtt
     3481 cttgcactac aaatgccgct tttagagaag gatacttgct ttttacaaac tcttcaattt
     3541 tttcacccat atatccgata atgaatacaa actcatcata cccggactgc acaaaataat
     3601 caatgatgtg ggccagaatg ggatgaccag caacaggtac caatgtttta ggttgtgtat
     3661 gtgtatgtgg gcgaagcttg gttccaatac ctgcaacagg aattactatt ttcatttcaa
     3721 attattttaa gcaaaaatat taaagtacag aacagtacaa gtctgatggc atggaataaa
     3781 aaaaaatgta gttttgccac acttaactga tatttaaacg cataatatgt tgaacaaaat
     3841 taccgaatta cttggcaagg atgccgataa attattaaat cacgtttgta aaacggttcc
     3901 aaaggaaaca ttacatcagc cgggcaatga ctttgttgac cgcattttta tcccgtcaaa
     3961 ccgaagcaat caggttatgc gtagtttaca gtcactttat aatcatggta gattgtcgaa
     4021 tacaggctac atgtctattc ttcctgttga tcaaggcata gagcattcag caggagcaag
     4081 ttttgctcca aacccaatgt tttttgatcc ggaaaatatt gtcaagttag ccattgaagg
     4141 cggatgtaat gcagttgctt caacttatgg agtactggca tctgtttccc ggaagtatgc
     4201 tcataagatt ccatttattg ttaaaatcaa tcataannnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnngatt taacagcaca ggcaaatcat cttggagtaa ccatacaggc
     4381 cgatatcatc aaacaaaaac tacctacaaa taatggtgga tatacagcaa ttaatttcgg
     4441 taaaacacat aaggatgttt ataccaaact ttcttctgac catccaatag atttatgccg
     4501 ttatcaggtg ttaaattgct ataatggacg tatgggatta attaactcag gaggtgaatc
     4561 aaaaggtgca acagatatgg ccgaagcagt aacaacagca gttatcaata aaagagccgg
     4621 tggtcaggga ttgatttcgg gacgtaaggc atttcagaag ccgttcaaag atggtgttga
     4681 aatgttaaat gccattcagg atgtatatct ttcaaaagaa gttacattag cataaaattt
     4741 taaacagtac atcagttgat agacccaaca tatcattaac gtaaactgat tcatacatga
     4801 catggtttaa aagaattaaa gaaggtataa ccacttccac caaagagaaa aaggaaactc
     4861 ctgagggatt atggtataaa tgccctacat gcaaatctat tgttccatca ttggagcatg
     4921 aagaaaataa atatgtatgt ccgaaatgta attatcatga cagaataggt tctgaagaat
     4981 attttgcaat acttttcgat gataatcagt ttacagaatt taatgcagca ctaacctctg
     5041 ctgatccatt aggttttgtt gatacaaaaa aatataccga tcgtttgcgt gataccattt
     5101 ctaaaacaaa gttgaaagat gctatgcgta ctgctgttgg taaagtaaac ggaaacaact
     5161 tagttattgc ttgcatggac tttcagttta ttggtggaag catgggctct gtaatgggag
     5221 aaaaaattgc aggagcaatt gactacagta ttcagcataa aatgccattg atgattatta
     5281 acaaatcagg tggtgcccgt atgatggaag cagccttttc cctgatgcag atggctaaaa
     5341 catctgctaa actaacaaga cttgcagatg caggcttgcc ttatatcagt ttacttacag
     5401 acccaacaac gggtggtgta acagcatctt ttgccatgct gggtgattta aacattggtg
     5461 aacccggagc tttgattgga tttgccggac cgcgtgttgt gaaagaaaca ataggtaaag
     5521 atttacccaa aggatttcag acttcagaat ttgttcttga acatggtttt ttagataaaa
     5581 ttgttgaccg cagacaacta aaagatacca ttggtcagct actgagcatg tttaaaaatt
     5641 agtctttgca tatttctttt ttgactacat aattaagaca atcatttact gattttctat
     5701 ttttacaagc aaacattgtt tatgctgtaa acgttatcag taactattaa aaatcaaatg
     5761 tatcgtagag agtttttatc atcaattgca ggcattggag ctattggaac tttaagttct
     5821 tttaaaaata ttactgatga cctgagtaat tccgataaga tgcctgtgtt atttatcggt
     5881 catggctctc caatgaatgg cattgaaaat aatgagttca gtaatcaatg gaaaaaattt
     5941 ggtcaatcgt tacctattcc aaaggcaatt gttattgttt ctgcacattg gcttacaaaa
     6001 ggcacttgga taactgcaat gaaaagccct aaaaccattc atgattttgg tggatttcca
     6061 aaagaacttt atgatgtgca atacaatgca ccaggcagtc cggagttggc acaggaaaca
     6121 aaaagtataa tcaaatcagt aaatgcagaa ttagaccacg attgggggct cgatcatggt
     6181 gcatggactg taaccagacg aatgtatcct gatgcaaata ttcctgtgct tcaaatcagc
     6241 attgacatta ataaaccggc agcctttcat tatcaactgg gaaaagaact tgcatcactt
     6301 cgaacaaaag gggtgttagt aataggaagt ggtaatatgg ttcacaacct cggaaaaatt
     6361 gcatgggaca aaattgatga agttggattt ggttatgatt gggctgttga aatgcatcaa
     6421 ctttttaaat ctaaaattac agataacaat catcaggcac tgattaatta tacttctctt
     6481 agtaaatctg cattactggc tgtacccaca ccggatcatt attttccgtt aatgtatatt
     6541 ctcgggttgc aacaaaaaaa tgaaaaagca gaatttttta acgataagtt agttgccggt
     6601 tcaatcaata tgacttctgt aaaattcgga taatcgaaaa ttacaacagt tagtcttcta
     6661 accgtttctt ctttttatca ggaactatcg aataagtaca taaaggtagc ccatcaacaa
     6721 aaacgtccaa tgtccttttc ttatttgatt caaatacata gtcaaaatac aacagactat
     6781 caacctgagt aaatgtgttt tgaatcttct cagatttttt tccgtcacga atttcaatct
     6841 taccaatagc aaggctatcc attgatttga aataaaagcg tttcttctgt cctttgaaaa
     6901 caggtatcac accatcagca ggtcttaatg cataaatgcc gttatcaaaa aagaaatcat
     6961 aaactttagg atacctgaga aaagtgttct tatcaacaat tgtatctaaa tattgacggt
     7021 ttccatttaa tggaagatgt gtcagaatta cctgacgcgg atgcaatgca aaatatctct
     7081 cagtaaagcg ttttgtaaaa atcttatcac cattttcaaa ataaccactt gccatcatca
     7141 cgtccatcaa ataccattta tcatatagtt ttactgtatt ccagatatga tttggttttt
     7201 taatttttgt tccggggaat tcattttgtc tcaaatagcc atttaccgat aggcatgtga
     7261 tattcatttt agcgcagatg gcattcaaaa tatttgcata gccttcagct aatgctgttt
     7321 tgtttttaaa aacttcgttg gcattgctat ttgatttctg cggatgacgt ttgtttttta
     7381 tgtcatacgc aacgttttca gttacccatc tgaatgcagc cctgaaacga aaatgtggac
     7441 ggtcaaattc tctgcttatg acagcagcta atgtatcaat gttaacgcat tgttctggtt
     7501 ttaattttag tacaaactca tcaacagaac cataatccag tacgggaatt ttgattcgct
     7561 tttgcgaaaa aagtgaggtt gacagaaaca aaaaaactat tgccgtaaaa aaacttttat
     7621 ggagcatgaa aaggttttgg ttaaaaattt tgaccaaaat accacattat tagtctaaag
     7681 tcaataagct ttaagacgaa ccggaatttt ttctttttaa aagcgtaatt ccaacgattg
     7741 aaacaataat acatgcaaca gcaatgagct ctgcctgtgt aatagccttt ccaaatatgt
     7801 ggtactttgt attgaccctg atctgttcaa ttgctagtcg ttctacacct gtgaaaagca
     7861 agtaaacaaa gaaaagaaca ccgggggtta ctattcttgt tcgtagtttc cataaaataa
     7921 agaaaagaag aatacatgca atcgcttcat atagtggcgt tgggaatacc ggtggtatca
     7981 attcaaaaca atgtttacca acacaacctt caataggaac accttcgcca attacattat
     8041 gcggatagtt gtaactccac atccagtcag gaaaaggaaa ccaagatggc tttgctaatg
     8101 tgtttacaat tccccagtca ccatcaccag aaaagtggca acctaatctt cctgtaccat
     8161 aagtcagcat tagggcaggt gctgcaacat cgcacatcac taatggcgga acatttaatt
     8221 tcttgctgct ataccaaatc actgctaaag caccacaaat caatccacca aaaaatgtca
     8281 atccactgaa tgaaatcaag gcatcaacag gatctttaag aaaatcatca atattctcaa
     8341 ggttatgaaa tattttagcg cctattaacc cggctacggc agctgcaatg gtaatattca
     8401 ttacaaactg atgtgggtag ccaacttctg ttacagtttg gggaactgct aatttttgtt
     8461 tttctttttc cttgtatttg ctccacaccg aatatgcacc gagtaatatc cctccaatga
     8521 cattgcctct tgatgataat aaaaatcctt gtggatcatt aacaaggtca ctgtaattca
     8581 aaatggcatc gagcaattta aagccaacta aaaatcccac agcacctgac caaaacaact
     8641 ctgaagttga cgcaggtttt cctttaatgg ttgtaacggt gaaagacttt aataaaccat
     8701 ttcgctcttt acgcttaaac tctaaaatca aaatatatgc agccagaaaa aatgataatg
     8761 ccaaaatgag cccgaatgtt tgtattggca atgggatgtt tatgcccagc aaatcatgga
     8821 gtgcatcgga taaagtaggg tacatggaca gaaattatta agccgggata gattctaaaa
     8881 ttgtacagtt aacttcatga ctgtcattgt cggttgcacc gattatacat tctgtcactc
     8941 tgtttaccat ttggttattg aaaggtgtgg taactcttac ataattttcg gaataacctg
     9001 acattattcc attcttgtct tcgtgttcaa aaagtacttt ggctgttctg ccttgttgtg
     9061 aacgatagaa tattccgagt tttttctctg aaagtatgcg cagcatattg gttcttctct
     9121 ttcgttctgc aatcggaaca acaggtttca tttcaatagc aggtgtgttt gctctttcgg
     9181 aataagtaaa aacatgcagg taagaaatgt cggcttcatt caaatattga tatgtctgaa
     9241 ggaaatcttc ttctaattca ccgggaaatc cggtaatcac atcaacgcca atacaggcat
     9301 cgggcattaa cgctttaatt ttatttatcc gtgttgtata atgtgaagtt aaatatcgcc
     9361 tgcgcatcag tttcagaatc ttatcagagc cactctgcaa aggaatgtga aaatgaggca
     9421 taaatttttt tgactggctt accagttcaa taatttcatc tgttaatagg tttggttcta
     9481 tagacgaaat tctgaatcgg atatcagcat ctaaagcatc taatgcagaa accaactgaa
     9541 aaaattctgt tgtgtgcttt ttctcaccgg gaagtttgcc aaagtctcct aagttaaccc
     9601 ctgtcagtac gatctctttg gcacctctat taacaatttg ttttacattt tcaataacat
     9661 tctcaacggt attgcttctg cttgcaccac gtgccattgg tatggtgcaa aaagtacagc
     9721 cataatcaca accatcctgc actttcagaa aaacacgtgt tctgtctcct tcggaatagc
     9781 tgcttgtaaa ttgatgatgt tcggcaatgt cgcaagaaaa aacctgcgct tctgacttct
     9841 ttttaagctt ttgtaaataa tgtggaatgt taaacttttc acttgctcct aatacgaggc
     9901 taacacccgg tattgcggca attgcatccg gttttaactg tgcatagcag ccaattatga
     9961 taacacaacc ttcggggttt gataataaag ctctgtttac atgttgccgg cactctctgt
    10021 cagcattttc agtaactgaa caggtattga tgacataaat atctgcggca tctgaaaatg
    10081 acactttttt gtagcctgca tcttgcaact gacggccaat tgtcgaagtc tcagcaaaat
    10141 ttaatttaca gcctaaggtg tgaaaagcta cagtagattt cattgtcatt atcgcgcaaa
    10201 aatagcaatt acagatgaac tttacctgcc gaaacagagc atatcatttt gaactaatca
    10261 ataagagtgt caattttgca tcctattcaa aaaataatgt caacaagcgg aactgcattt
    10321 aactggaagg tattgaacag gattatgttc tatgtaaggc ctttccggtt tctttttgta
    10381 gctacagtgg ttatcactgt attgcttgcc gtactggcac ccttacggcc ctggcttaca
    10441 cagcaggcac ttgatattga cattgcaaaa catgacatca aaggtcttac acgtactgtt
    10501 gcattcatgt ttggtgttct tctactacag tcgctgcttc agtttgtata tacaacttta
    10561 acaaaccgac ttgggcagga agtaattctt tcattgcgta ataatttgtt taagaaggtc
    10621 agtgcttttg ggcttgccta ttttgataaa actccgcttg ggattacaat tactcgattg
    10681 gtaagcgaca tggaaacgat tgctgatatt ttttctgacg gtttgattct tatcatcagt
    10741 gatatgcttc aggtagtggt aattattgct gtaatgtttt acatccatcc cttactagct
    10801 gcaatttcat tatcaacaat ccctgttctg ctgcttgcta caagagtgtt tcagaaaaac
    10861 atcagaaaaa ctttcaatga tgtgcgcata caggtagcaa aattaaatga gtttgttcag
    10921 gaacatttag ttgccatgcg tattgtacaa ctctttaacc gagaagaaga ggagttaaat
    10981 aagtttcatg acataaatgc taagcatcgt gatgcaaata tcagatcaat atggtattac
    11041 agtttgtttt ttccggttgt agaaattctt tcagctgttt ctattggact aattgtatgg
    11101 tatggtggtt taaatgtact tcaaagttct atcagccttg gcttgctggt agcatttatt
    11161 cagtacatca accagttgtt cagaccgatt cgtgagcttg ctgataagtt taacacatta
    11221 caaatgggca tggttagttc agaaagagtc ttcaagctgc ttgatgaacc atttacagaa
    11281 gtcaactcgg gacaaaaaag tgcagataaa ataatcggaa aaattgaatt cagggatgtt
    11341 tggtttgctt ataaaaatga ggagtggata ttgcgtggtc tctcttttac tattcctgca
    11401 ggacaatctg tggcaattgt tggtgctacc ggtgcaggaa aaacaaccat cattagctta
    11461 attaaccgct tgtatgagat tcaaaaaggc cggattttaa ttgatgacat tgatatcaga
    11521 gaatatgatt tatacagcct gagaaatgct attggtgtag tccttcagga tgtctttctt
    11581 ttttctgaca gcataaaaaa caatattaca ctctataaag aacaagatga cattgtgatt
    11641 gaagccttgt tgaagcaaac aggagcttgg cattttgtag aaaagttacc cggtcagctt
    11701 cattacaatc cgggtgaaag gggagggtta atcagtaccg ggcagaggca gttgttgtca
    11761 tttgccagag tactggcaca tcatcctaaa ctaattattc tcgatgaggc aacaagcagt
    11821 gttgacagta agttagagca gcttttggta aatgcaacgg aagtgctcac aaaaaaccgt
    11881 acttcaatta ttattgccca tcgtttatca acaatagaaa aggccgaccg tatcattgtt
    11941 ttacaaaaag gtagcattgt cgaagacgga aaccatcaga ttttattgca aaagaaagga
    12001 gtttatgctc accttcatga ggttcaattt atgcaaccat aattaatatt tataagaact
    12061 gatttttgtt tctttgcaca tcaccaacaa agtatgaaga tattttttag tttattttta
    12121 acactgtgtt ttgctgccgg cacaaccttt ggtcaagatg cggcaagtac tgacaccata
    12181 catcacaatc acttcaacga tggtgtttat cttagttatg aagatttaaa gagtaacaga
    12241 cccactatta aaaaagaaca actcatcaaa tcttattatg actcttctgc tttcagcata
    12301 agcaagtggg ccaatacgca aaacttatat tatgccacat cagcaggcga caaacaaaaa
    12361 gttaatcgcg acagcctttg gggctatgtc gaaaacggaa taccttacat ttttttaaac
    12421 gatcgctttc ataagttttc tacctctggt gcggtttctg ttttcagaga gagctatcct
    12481 aatttaaaag caggattagc acctgtggta acagaagcta aatatacttc acaaatcaat
    12541 ttatttattc tagctaacgg caaaatagat gaatttactc ctgaaaatgt agcccttgcc
    12601 attgaagaca acaaagctct ttacgatgaa ttcatggcat taaaatcatg gaagcaaaaa
    12661 agaaaaaaaa tgtatcgttt tgttgaaaga tataatgata gtgcaatgtg gtagtaatta
    12721 cttaacataa gatcaatgaa aaaaataaaa ctcctattcc taactgtatt catttttgtc
    12781 gtggcaatag tgccgcaagg atgtaaacaa aaagttgctg aagtcaataa ggccggcttc
    12841 gtaagcatta aagaccagaa attttattta aacggaaagc cattttttcc gttggcggtg
    12901 aattatcgtg tggcattgca aacagacgga caacagcttt ggccatcggc atataaaggg
    12961 tatgatgaag gtaatcgtta ccgtttcaca aacaaagatt caagtctgga agaattaaaa
    13021 aatgattttc aactcattgc tgatatgggg ttcaactcga taaggattgt tggcgttggt
    13081 gaacaatatt ctgttacaaa aaattctgat gagttatata ttaaagcgga tattggcaat
    13141 gaaagagaca ctatgtttct ttttaattct caagaaacat acaatttgta ttataatgca
    13201 ttaaatgaaa tgatgcttgc tgcaaatctg gctgggttaa gagttgtttt gcttgcaaga
    13261 ttatttcatg aagtaccggc aacagaaaaa cattggggca gaatcatgaa acattttcaa
    13321 ttggatacca tgttaatggc atacgatttt tttaatgaac ctttatattt cgattcgctg
    13381 gaaagaaaca aaacagaacc ttatcacata accaaagcct ggaataagtt agcaaaagaa
    13441 aatgcaccgc atcaacttat tactattggt ttaacgggta tcagagaagt ctttgagtgg
    13501 gatcccaata cgctgaatgt tgattttatt tcttatcatc cgtatgaata cgaaccagaa
    13561 caagtacgta atgaaattta ctggtatggt aaatatgtaa gaaagccatg gattatcggt
    13621 gaaacatctg tgccggctga taatgattcc gtgccttata gtgtgcagaa agcatttgca
    13681 gaggctacct ttaaacaaac ctgcaattgt ggtggtattg gttatacgtg gtggcaatat
    13741 aaagatgtag attggtttga ctttcattct aattttatgg gtattatcaa tcgaaaaggc
    13801 acaacgcgta catccacaaa caaagaagta attggaacac ctaaaccaat aactgaattt
    13861 tttaaaatag caaaaccttt tatggatgct tcacaatgtt taaagttaga gaattactac
    13921 aactattcaa atgcaaatgt atgttgtctg aagggtaaat tagttggctc ctccggtgaa
    13981 cctatagaag gtggtgtggt tatggcatgg agtaaagatt gggctaactc ttatcatact
    14041 gtaactaaac aagatggaac attcgagctt ttaggtagtt ttccatttta ccactggata
    14101 gcctctgcaa ccttgtttac tatggtaaga ggtgatgttt tgccagacag ttctgacaat
    14161 aaaaatagta aaatacttac cttagatttg ggatctttaa atttgaagaa actaaactta
    14221 gaataaacaa tgatataact aaatactatc agtattaaag taaagaacgg agaattacga
    14281 ttaaagaatt attataaaaa atgaagttag ggagcaggcc aataattgtt gttataattt
    14341 atctgattat ctattcctcc atatcattgg tgaatcatta taatttcaga acttttgcat
    14401 gggatttagg cattaacaat aatgctattt acgattatgc gcatttcaga tggaatgatt
    14461 gcatgatatt acaacctcag tttaaaaatg ttttagcaga tcattttact ttattgccca
    14521 ttttagtatc accattatat tggttttttg gttcttatac tatgttgcta tttcaaataa
    14581 cagcaatact atttggtggt gttggtattt ataaatattt tttacataaa acctctgact
    14641 ccagcctttc tttaattgca atgattcatt tttattcggt ttggggtatc ttttcagcct
    14701 taggttttga ctatcacgat aatgttattg gatctatgct agtaccctgg ctcttttatt
    14761 cctttgaaaa agaaaaatgg aaaacagcag ccatttattt tttactaatt tgtatcagca
    14821 aagaaaatat ggccttatgg gcagtttcaa tagtaatgac catttatctt ctaaattaca
    14881 aatcaacaag agtcagaaaa atatctgtcc tatttggttt attcgcctta gtctattttt
    14941 tgtttgtagt taaggtaatt attccttcaa ttgctaatga aggtcgtgaa tatttgcatt
    15001 ttaattataa ggcaattggc aataatttct ctgaagcaat tcaaactcta attttaagac
    15061 ctcaatatgc ctttagtttg ttatttgaaa ataccacagg ttggaatgaa gcaaatttta
    15121 tcaaatctga attgcatttt tttgtgctac tttcaggggg atatgccatt ttatataaac
    15181 cacaatactt gttaatgttg ttacctattt atgctcagaa actatttaat gatgatatag
    15241 gtaaatgggg tttaaattat caatactcta tagaattagt ccctatttta actctcgcac
    15301 tttacacttt tctatatgaa attaataaac gacataaaat ttattactta ctgcttcctt
    15361 gtttgattac tattgtggca acagcatctt caatggatca tcgtatttca aaatggtacc
    15421 aacctgaaca agttcgattt tatgacaaaa aacattatca aacagaatat aatgttgctg
    15481 agataaatga aaggctaaag gtaattccaa aaaatgcagc cgtttctgct tcgaattgtt
    15541 tagtccctca tttagcattt agagattata tttatcaatt tcctgttaca aacaatgctg
    15601 aatatattgt attgttgaaa tcagaaaaca cttatccatt aagccatgaa gaatttgaaa
    15661 acaaaattag tgagtacaat acatcaatcg actggaagaa ggttgtcgat gatgataact
    15721 taattatcta taaacaaaac aaataaagag gttattaata aaatcttacc tcaatatcgt
    15781 ctgcatacac gttcaaattt gtatcactgt aaatatatgc gcgtagaaaa ttctcagagt
    15841 tgtttctgtt gttagttaac agatcaaatt caaactcaac atatgtccat tcatccacaa
    15901 catgcatttt gtcattggca ttacttccaa gccattcttt taaattgaaa tctttatctc
    15961 taatttcaag tgtgaacttt aaggctgaat taatttgctc ggttaaaatc caggcagata
    16021 acaatgcttt tttaggattg ccaggaaaaa catctgaaag acctaaatag aatgttccgg
    16081 aaaagggact ttccttatta agtttgtaaa catagttccc tgaatgtgca ttgtcaaatc
    16141 taattatatt attcactttc ttttctgaat gaaaggctat ttcagaatca gctgtgtcca
    16201 aatacgtaaa ttgttttttt gaatctttac aactcactaa aatcaagcaa gaaaatatta
    16261 gagtgatatt tagttgtttt ataattgata atttgttatt cgccatatct ctttgactgt
    16321 tcatagttag gatgtattaa agaattttca ttgattttat attcatttag gtttaaaaat
    16381 atttctttat atgcttcaaa agtcatggca tcgtaatgta aaaaaccttt atctaattga
    16441 aatgactgtg taatgttcaa gccaattgaa gaaagtaaaa ataagataca tacaatagcg
    16501 taaaattttt ttctaataat gaaagaaaaa aagcagccca ttacaagtgc gaataaggca
    16561 taaaaatcaa ccaatgttct tgcactaaac gacccaccat accaccaaca ccaccacgaa
    16621 aatgttaaat acaaaaaaat agcagttact attgaaaaag cccagaatat atttttcatt
    16681 cttttatagg aaataaataa accaggtatt agaagtatca taacaggagt atatataaag
    16741 aaccctttcc gaaaaccgaa taaaccttca aagacatgag gtttatcaag aaaaaattgc
    16801 tcttgcccat agctgtagaa caaatatgta tcacaatgta tcttccaata cactaattga
    16861 ggtaaaaatg gaagtaaaaa gcaaaagaat gcaagaaata ctcttgaagg tttagaaaga
    16921 attactttca ttttttgtag caagttaaat ccagctttgt tataaaaaat cggaaacaaa
    16981 cagattatcc cgtttaatgg acgaattaat gttatcaacc caccaattat acttaatata
    17041 attaatgatt taatattaaa atcatcatga ttccatctta tggaataata caaaaaaaca
    17101 gaaactaaag aaaaagaata aacatgagac ataccactct caaaaacagt gtaataaaag
    17161 agattagtac ctaaagaaac tattacaagc accaatgaag taactatatc atcaaagaat
    17221 agtaataatg ttttccgtaa atagattagc cctattaata agtaaaacaa tgctgcaact
    17281 acaattgaaa gttgaaattt actaccaaaa ccagatttat tttgtccatc agatgtgtgt
    17341 atgttttttg caactaaaaa aaagggtgaa caaagtattg cgactcccga agtcattttt
    17401 aagtattttt tattatcact taatttttca taccaaatat tattagtaat tgttgaaggt
    17461 aattccgata ggaagtctaa agacaaatca tgataaataa aggttgcagg tagataagta
    17521 tagtatatca aactatcata ttttaaaact tcttcttttt gccacttttt atttataaaa
    17581 gcatacccgc aaaaaacaag aaaaatcaaa agaattgaaa acttacttat gatacacttg
    17641 tttttcatta ataccaattg ttcaaatttt attttactat gtatatcgtg ctatacacca
    17701 cagtgcccga aagccatctt tccatccgat tttttttcct tcggcat
//
LOCUS       DAOQNP010000067        17712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1125_length_17712_cov_10.420740, whole genome shotgun
            sequence.
ACCESSION   DAOQNP010000067 DAOQNP010000000
VERSION     DAOQNP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17712
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1125_length_17712_cov_10.420740"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..942
                     /locus_tag="PKY09_09495"
     CDS             292..942
                     /locus_tag="PKY09_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNB13132.1"
                     /translation="MKKLFILALLVVSSISINAQKLKEGTAHYKIDYNMESDEGGMKG
                     MLPTDANMSFKGDMFMVETTGGMFDQKTISDSKKQESILLMDMMGNKVAVKITKADIE
                     KEKNKGTKPKVEITNETKTIAGYNCTKAIVKAEGAEPAEIWFTKDLAINNGFSQGYDG
                     IDGMMLEYTIPQKMFTMKMTCTEIKADKVDDKVFEVPSDYKPMTKEELMKMSGGGH"
     gene            complement(1240..3609)
                     /locus_tag="PKY09_09500"
     CDS             complement(1240..3609)
                     /locus_tag="PKY09_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNB13133.1"
                     /translation="MKSIITIVLVLLINANVFAQSEKYHKVRIWLEGKAVSSLAQTGI
                     DLSEAHYRKGLYFETDLSASELNRVSNAGFRTELLIDDVKQFYKQRNEQQNQKTTAAV
                     NSCITAPDYPQPAHFGYGSMGGFFTYQELLNNLDSMASLYPNLITVKQPLSNTLTTVE
                     GRPIYYVKISDNPNLSEPEPEVLYTALHHCREPNGMAAVIYYMWYLLENYATNTDIQQ
                     LVNNTEMYFVPCVNPDGYVFNEMTDPNGGGMWRKNRQQFPGGEYGVDLNRNYGVQWGF
                     DDVGSSPDPAEDTYRGPSAFSENETQMISAFCQQHNFKLALNYHTYSNLLIYPWGYIP
                     DYYTPDSALYKNYGDILTTYNKYAAGTANQTVGYMVNGSSDDWMYGEQTLKPKTFAMT
                     PEIGSADDGFWPQQSRIVDLCKENMYANIMLARLAGKYGEATDKSKWNIPALTSYIPF
                     DFKLLGLDTVGSFTVTLTPLSANIQTTGAAKTFSGLNFNQSVADSISITVNSTTLPGD
                     EVKFLLSVNNGLYDISDTITKYYGPAVTPFFENGNTAAAWNTQFWGAATSSFVSAPSS
                     IADSPIGDYQSSSNNEITTISSINLLNAAAARLTFFAKWATEPQYDYAQLQISDNNGS
                     TWTPLCGKYTVPGSPFQDLGNPVYQGVNLNWVKEDINLNTYIGKNVKLKFIMVSDGFV
                     EYDGIYIDDLSVEKIIASGVGINNHENNTFNIYPNPATDFARIETAANAAHIFITNAI
                     GQMVFSESLNKEQQHYDLNIADFAAGVYVVKVKYNSGAVAQKRLMIYKK"
     gene            complement(3710..5056)
                     /gene="tilS"
                     /locus_tag="PKY09_09505"
     CDS             complement(3710..5056)
                     /gene="tilS"
                     /locus_tag="PKY09_09505"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNB13134.1"
                     /translation="MLLRFQKFVKEKKLFSKEQTILVAVSGGIDSMVLLQLLQQSGYK
                     IAVAHCNFKLRGQESDDDAQFVHSFCLENKIVIHATEFETEKLSEQYQKSIQVTAREL
                     RYNFFQELCSRFHYDFIATAHQADDVAETILINLSRGGGMAAWHGIEVKRENIVRPIL
                     FAQKHEIEKYAKENKIQWRTDSSNQKEDYTRNFIRHQIIPILKKINPSVVQTLFHHAE
                     IMTEAEKIILEQIEKFRAVSVVSNGNKSIVPISDLLKATAPHTVLFYLLQPFGFNATH
                     VVDIFNTLNHSESKTFHSDKFQLIKERDQLIIVPLEEKKTSIYFEIPTTGTYAIADSQ
                     ISVELLENTADCYNSIVKNNNNAICYFDTEKLEFPIVVRTWQYGDYFYPLGMHGKKLV
                     SDFFTDEKMSQSEKQMQLLFLSNSEVMWLEGRRISEKFKVEKNSKSVLKISVKAKV"
     gene            complement(5085..6356)
                     /gene="pabB"
                     /locus_tag="PKY09_09510"
     CDS             complement(5085..6356)
                     /gene="pabB"
                     /locus_tag="PKY09_09510"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNB13135.1"
                     /translation="MSDTKFSPAEFEELLKTSNGFRNFCFLNSNSAAEGSYSGLTKYD
                     FLAGINALDTVEATSKSLEKLEAFIQKNKGKWMFGFMSYELKNEIEQLKSSHTDRLNF
                     PVLHFFVPQVVLVSEGGKLRMIDQLEQTTKQFVADDVELRQMHASGNVALTPRFSNSD
                     YIQTVEKLKEQLQQGNIYEVTFCQEFYNDSANINPFETYIKLNANTRAPMSCFYKKEN
                     QFIISSSPERFMCRRENKIYSQPIKGTAPRSSSREEDEWLKDNLQNSEKERSENIMIV
                     DLVRNDLSRIAKPGTVEVNELLGVYTFKTVHQLISTVSCEVEPNISFSEIIRAAFPMG
                     SMTGAPKISAMTLIDAHEKSNRGLFSGSVGYIDPAGDFDFNVIIRTILYNAQRNYVSV
                     QTGGAITIQSDPAAEYEECMLKAKALMDVLG"
     gene            complement(6471..7556)
                     /locus_tag="PKY09_09515"
     CDS             complement(6471..7556)
                     /locus_tag="PKY09_09515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNB13136.1"
                     /translation="MNQIIQLFFPFISSLIIASLIIPSWIQLCSKWNLFDRPDTRKHH
                     TAIIPSLGGIGIFIAIMISIFLFGYNSQDTSFHSIAAAMLILFITGFFDDLTDLKARV
                     KLNIQLLAALIVTYSGLRIQTLNGFAGVHELPILTQYGISIFIILFLVNAYNFIDGLD
                     GLAATIGLIIFSIFTVLFHIHGEQAYAVLCVSVIGALLSFLYFNFNPARVFMGDTGSL
                     VVGFLIAICTIKLFNIWLASPVVSFGPSLTIATIFILIFDLTRVVFIRLSNGISPFKA
                     DRSHLHHMICRQQFGHRGGTFIMAAFNILFIVLVLPLSKLRFITFLIFCFCLAILLMN
                     SKVMSYVALLRNKITGAPESKMGVDRG"
     gene            complement(7566..9056)
                     /locus_tag="PKY09_09520"
     CDS             complement(7566..9056)
                     /locus_tag="PKY09_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNB13137.1"
                     /translation="MKRIIIIATFCMLSGIVNAQDPHISQYFNVPVFYNPALSGNGIQ
                     NMRIAVDYRNQWLAPGLSNKTQTIALDKIVNKVGFGLMVSKNSAGKDGFSRLSVLGGI
                     SYHAKFGYQKRHQLSSGFQVGILQKSFDPNKLTFESQYTPDIGYDPSASNGETFANTK
                     VTRPDINIGIAYHYAQGNKEILIKPFAGVSFSHINKPKESFIEEQNVNPIRMNFHGGA
                     SITCSKTIELKPLVYFMKQDGFQEMSAGMISELSLSNNSLVKLGLLYRNNDAMVAYAG
                     YQFNKVFLGTSYDFNISKYKPGGSGAYEISLVYTPQGRAKGKRNEDRKPEPVNKKKLP
                     EQRVTPPQALTPTPVIVPSIIEQNPIVVSLAEMPMKGESKIKENTELSNYNYQTIGYV
                     VFQDYSLKYDINNRFDVIEATIDYLYKNINRKIIVSGNASEIELKKSSNIGKQRAEAV
                     KVYMVGKGIDADRIITVDVKDKMPALKTEDGVQIERLSRTDILLIK"
     gene            complement(9066..11567)
                     /locus_tag="PKY09_09525"
     CDS             complement(9066..11567)
                     /locus_tag="PKY09_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB13138.1"
                     /translation="MIRAGIAIWAVMLCMLTANAQNVVTLAGQNGVSGAANGSGTSAS
                     FNNPHGMVCDQAGNLFVADRNNHLIRKITPAGVVSTFAGSGSAGSSDGIGAAASFFEP
                     WGIAIDQSGNLYVADTKNYLIRKITPTAAVTTIAGVGTSGVTDGPASSAQFGFPTGIA
                     VDASGNIYVSEFMTHVIRKISNGNVTTFAGTAFQSGDANGTGNNAKFDHPHSLAIGQS
                     GNLFVTDTWNNKIRKITPSGVVSTYAGSTAGYVDGAASVSKFDNPLGICADASGDIFV
                     GDVNNFCIRKISNGTVTTFAGTAGTSGSANGPLLQASFNSPSCMAYHSSDKYFIGDEG
                     NELIRLINMNQSSNPLTLSTLNNVSVFCPSSTSVITASPQGLSSYTFKEGSTVLATNT
                     TGILSLGTLSAGTHLISCTATGPLGNYATTTALSITIVDASTPVISPTGPISICSGGS
                     ATLTASPGIAYLWTNGATTSSINATLPGTYAVSITYTGGCSYQSNSVIINLNGSFSAA
                     ISPATAQVICPGDSVKLTATAGTSYLWSNGKTTQSIYAKTAANYTVAVTSSFGCTSVS
                     PPVNVSLKTMPSSIEASSDSSCSGEGVLLTITQQSNTTYSWFDEAAGGTALATGSSYA
                     TAPLAESTTFYVQLNSNGCTSVSRFPVLAYIYPILHVDFAESQAVKADGGYAVSFQNN
                     TPTGNNTYTWNFGDGSVNAISNEENPQHIYSSEGDYSVTLTVKDEFGCENSFTKNISV
                     TVAHDLFLPSAFTPNSDGINDIFRLKGSGFVSADMLILDQWGQLIFRTNNAVVGWDGS
                     VKGNLAPNGTYSYIVKIKLNDGSDKVLKGNISIIK"
     assembly_gap    11754..11853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11854..13628)
                     /locus_tag="PKY09_09530"
     CDS             complement(<11854..13628)
                     /locus_tag="PKY09_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13139.1"
                     /translation="MVNRIIYILVFLLISINQNILSQSNATRLFPQEKINEALNKFIA
                     SAQWSNQNKHASQSPTGFVSSPASVLFKKVFDNNEQDLMVADTIIVGAQINDTLVITG
                     NFTHTGPILVFNNGVLIFNQATVVDTGEIYVFGNGKLFADSTLFTFPQQYIYERSLIV
                     AQNGFVQIKNSSFNYSGMSHNLYLADSAVVEFTNIHQNDFTTCGLYGNANININGCNI
                     TGEYILTDKCQATFKNADTLILWHHFPDTAVVNYSFPPGDTVYNYVCNNSTPGISGIG
                     YQVSADSCHNVMWALMPVNGSDVTISNSNLRLIGCWFERGDVASVSGIFNNSNYSNYT
                     APLADRNLHLINTNVQTWSFYVFDSSQVDIDSCQLGEVGTQQSAFVNANNLLLDGSGG
                     YFWATDSSGILALGTTIYTMARSEKKGIFILAYGWQPFMAPTALSNSLMVCVQSTLGQ
                     DPVPYDGAVAWMQNISSPDTAIINTIVPINGSAWIDQGPSGAVMDFDNYSLYWSVMTS
                     NWAPIVSNVIAEVHNNGLLGSWNTNGLVSGNYFLRLVMKNNLGDSLEAIKPVYLKQLV
                     SVNELTGQSLVIYPNPAQNKISISD"
     gene            complement(13764..14534)
                     /locus_tag="PKY09_09535"
     CDS             complement(13764..14534)
                     /locus_tag="PKY09_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13140.1"
                     /translation="MEKLKYFHPLNYMVRLLTLNILLIFLAGCVNNSDEKLVQKIDSL
                     QKSLQTLEQKIAGQNNQDTIKNDEQKEMDTSLTHQTVSKKILHAVEPKNIQDTLKSKK
                     QIQITTPSTKQTDKSTRAETSVFYYYQSTPKRVSVEITPWHDGKRELIFFDPFGNKTY
                     TINDIRRSFSSTTELKRFHKNGACSQAKISINPDASMYWHETEINFDENNNPQWKKEL
                     QFPQESTEQNLNNRWWWNAKSKNWVRQQIIEEQPVPQK"
     gene            complement(14588..14926)
                     /gene="ssb"
                     /locus_tag="PKY09_09540"
     CDS             complement(14588..14926)
                     /gene="ssb"
                     /locus_tag="PKY09_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNB13141.1"
                     /translation="MNTLRNSVRLIGNLGAAPEVKNLDNGNKVARINLATNETYVNAK
                     GDKVTETNWHNLVAWGKTAEIAEKFLDKGKEVAVEGKLTSRSYTDKEGNKKYITEVVI
                     NEILMLDKKD"
     gene            15118..15501
                     /locus_tag="PKY09_09545"
     CDS             15118..15501
                     /locus_tag="PKY09_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13142.1"
                     /translation="MKNEKTCLECGETLVGRADKKFCDDQCRSNYNNKANSDVTAEVR
                     NINNILRKNRRILESVIGADGKVKISKQKLSDKGFNFKYHTHQHQTLKGSVYKLCYDY
                     GYLPIENDLIYVIKWGNFTPQQDGK"
     gene            complement(15602..17338)
                     /locus_tag="PKY09_09550"
     CDS             complement(15602..17338)
                     /locus_tag="PKY09_09550"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNB13143.1"
                     /translation="MITKIFTAKRKFIVNVSLSLTVLLTLVFTGAQAQITVSIGSAGT
                     GTSNYWPNYYLYGYSYSQQIVEATEINSGGWGGGAALISKIRFKPTMSAPTVLWQDWV
                     VYFGNTSKTSFSSTTDWIPVASMTQVFAGTIPANVTANVWMELTLPTPFLWDGISNLV
                     IAVDENTPNWGNTPAWAGYTTTANKGIYYYNDTTNPDPLAPPVANGRNTSNMGLAQIQ
                     FEFTGATPCSGTPSPGNTEASAVSVCADSAVVLSLQNNPPVSGLTYQWQSSPDGIAWT
                     NFGTNYPIQNQTQMTNTWYQCIVTCTNSSQSDTSAPIQVLMNLFTSCYCASSANSAAD
                     EDIFNVTFGTLNNTSDCNTTASGVGSIKNRYSNYMSGAGAPTPPSVLRGTLVPFSIQI
                     NSCASYYFYMSKIFIDYNQNGLFSDPGEEVYVSPSYTSGPRTESGTILIPMSAVSGLT
                     AMRVITQETAVASAIIPCGSYSLGETEDYMINIGPTVGFQNNFQSKVNILNAYPQPTS
                     DMLNVEYTAEKGLTLITLSEISGRVLIRQTHANVTGKNSLQLDLSKVARGVYLLNVQT
                     NGSNNQMSIVVE"
BASE COUNT         5424 a         3287 c         2999 g         5902 t
ORIGIN      
        1 tttagggtga tggtagaact aattgatgaa atgattcgag aaaattttaa agaaaatctc
       61 ggtggaaagt taactttcat tttagcttct tcatatattt tgtattggaa gataaagtcg
      121 tttaagaact aaccaaaacc ataatttttt ttaaccttgt ttcatgctgt aaattgtatg
      181 aacgtggtag ggtattcctt ttacagtatg attaaaagat tttttttcat taaagtaaat
      241 tcttctcttt cttttattac tttcgctttc tcattctata aatctataca aatgaaaaaa
      301 ttattcatcc ttgcattgct cgttgtgagc agcatcagca tcaatgcaca aaaactaaaa
      361 gaaggtactg cacattataa aatagactac aacatggagt ctgacgaagg aggtatgaaa
      421 ggtatgctgc ctacagatgc caatatgtcg tttaagggcg atatgtttat ggtggaaact
      481 acaggtggta tgtttgatca gaaaacaatt tccgactcta aaaaacaaga gtcaatcttg
      541 cttatggata tgatgggaaa taaagtggca gtaaaaatta ccaaggctga tattgagaaa
      601 gaaaaaaaca aaggaactaa acctaaagtt gaaattacca acgaaacaaa aactattgca
      661 ggctacaact gtactaaagc aattgtaaaa gcagaaggtg cagaacctgc agaaatctgg
      721 tttacaaaag acttggccat taacaacggt tttagtcaag gttatgacgg tattgatggt
      781 atgatgttgg aatatacgat accacaaaaa atgtttacca tgaaaatgac ttgtacagag
      841 attaaagctg ataaagttga tgacaaagta tttgaagtac catcagacta taaaccaatg
      901 acaaaagaag agctcatgaa aatgagtggt ggtggccatt aaacttgaag gttgagataa
      961 ttccaatata ctttaatttt tagattaaat ataaaaccgc tgttatgttc ctgattgtaa
     1021 tcggggattc atgatagcgg ttttttaaat aagagatttg ttgaattcca gatttgaaga
     1081 atttttgtca gtagctttcc cattttattt tggtgtacac agttattctt caacataagt
     1141 agaatgaatt tttatatcag cagtattaat ttacgctgag atggagattt gcagaatttg
     1201 tatctgcata atctcattag acccccatca atctgtatcc tactttttat aaatcatcaa
     1261 acgtttctgt gcaacagcac cactgttgta tttaacttta acaacataaa caccggcagc
     1321 aaagtcagca atgtttaaat cataatgttg ctgttctttg tttaggcttt cagaaaatac
     1381 catttgtcct attgcattgg taataaaaat atgcgctgca ttagctgctg tttctattct
     1441 tgcaaaatct gttgcaggat tagggtaaat attgaatgtg ttgttttcat gattgttaat
     1501 tccaactcct gaagctataa ttttttcaac acttaaatca tcaatataaa taccatcata
     1561 ttcaacaaag ccatcactta ccataataaa tttcaacttc acatttttgc ctatgtaggt
     1621 attgaggtta atatcctctt ttacccaatt taaattcaca ccctgataaa caggattacc
     1681 taaatcctga aaaggacttc ccggtactgt atatttgcca caaagtggag tccatgtgct
     1741 accattgtta tcggaaattt gcaattgtgc ataatcatac tgcggttctg ttgcccactt
     1801 tgcaaaaaat gtaagtcgtg cagcagcagc attcaataaa ttaatactac tgattgttgt
     1861 gatttcatta tttgaagaag attgataatc gccaataggt gaatctgcta tgcttgatgg
     1921 tgccgataca aaggatgatg tagctgctcc ccagaattgt gtattccatg ctgctgcagt
     1981 atttccgttt tcaaaaaatg gtgtcacagc aggaccataa tattttgtaa tggtatcgga
     2041 aatatcatac aaaccattgt ttacggataa cagaaatttt acttcatcac ccggtaatgt
     2101 agttgaattg actgtgatgc ttattgaatc tgcaactgat tgattaaaat ttaaaccact
     2161 gaatgttttt gctgcacctg ttgtttggat gttagcagag agtggcgtta acgtaacagt
     2221 aaatgatcca acagtatcca atccaagtaa tttaaaatcg aatggtatat aggaggtaag
     2281 tgcaggaata ttccacttgc ttttatcggt agcctctcca tatttacctg ccagacgagc
     2341 aagcataatg ttggcataca tattttcttt gcacaaatca acaatgcgtg attgttgtgg
     2401 ccagaagcca tcatctgcac tgccaatttc aggtgtcatt gcaaatgttt ttggtttcaa
     2461 tgtttgctca ccatacatcc agtcgtcaga acttccgtta accatgtagc ctaccgtttg
     2521 attggctgtg cctgctgcat atttattgta tgttgtcaaa atatcaccat agtttttata
     2581 caaagcactg tcaggtgtgt agtagtcggg aatatatccc catggataaa tcaataaatt
     2641 actataggtg tgataattta atgcaagctt aaaattatgt tgctggcaaa atgcacttat
     2701 catttgtgtt tcattttcac tgaaagccga agggccgcga tatgtgtcct ctgccggatc
     2761 aggtgatgaa ccaacatcat caaatcccca ctgaacaccg tagttacgat taagatcaac
     2821 accatattct ccacccggaa actgttgtct gtttttacgc cacatacctc caccattggg
     2881 gtcagtcatt tcattaaaca catagccatc aggattaaca caaggcacaa aatacatttc
     2941 agtattattc actaactgtt gaatatcggt gttggtagca tagttttcga gcagatacca
     3001 catgtaatat atcactgctg ccatgccatt aggctcacgg caatgatgca atgcagtgta
     3061 cagtacttca ggttccggct cagaaagatt ggggttatca gaaattttta cataataaat
     3121 cgggcgacct tcaaccgttg ttaatgtatt gcttaagggt tgttttacag taattaaatt
     3181 aggataaagc gatgccattg aatcaagatt gtttaacaac tcctgataag taaaaaaccc
     3241 acccatgctg ccgtaaccaa aatgtgcagg ttgtggataa tcaggtgcag tgatgcatga
     3301 atttactgct gcagtagttt tctgattctg ctgttcatta cgttgcttat aaaattgttt
     3361 tacatcatca attaataatt ctgttctaaa gccggcattg ctgacacgat ttaattctga
     3421 agcgctcaaa tctgtttcaa aataaagccc tttgcggtaa tgtgcctcac taaggtctat
     3481 tcctgtttga gcaagtgatg aaactgcttt accttcaagc cagatacgca ctttatgata
     3541 tttctctgac tgtgcaaata cattggcatt tattaatagc accaatacaa ttgtaattat
     3601 tgatttcatt ttaattgaat aaattttttt gttgaattat taatatggct tttgcaataa
     3661 caaaactatt taaaaaatca ttgtttaaag taattttaac atgtttgttt taaactttag
     3721 cttttacact aattttcaaa acagacttac tgtttttctc cactttaaac ttttcactga
     3781 tacgccttcc ttcaagccac atcacttcac tattacttaa aaacaacaat tgcatttgct
     3841 tttccgattg actcattttt tcatccgtaa aaaaatctga taccaacttt tttccatgca
     3901 tacctaatgg ataaaaataa tcgccatact gccatgtcct tacaacaatt ggaaattcta
     3961 atttttctgt atcaaaataa cagattgcat tgttgttgtt ctttactata ctattataac
     4021 agtctgctgt gttctccaat aattctacac ttatttgaga atcagcaata gcatacgttc
     4081 ctgttgttgg gatttcaaaa tagatacttg tcttcttctc ttcaagaggt actataataa
     4141 gttgatcccg ttctttaatc aattgaaatt tatctgaatg aaatgtcttg ctttcggagt
     4201 gattcaaagt attaaatata tcaactacat gtgtagcatt aaatccaaat ggctgaagca
     4261 gataaaacag gaccgtatga ggtgctgttg ctttcagcaa atctgaaatc ggtacaatgg
     4321 atttatttcc attactaact actgatacag ctctgaattt ttcaatctgt tcaagaataa
     4381 ttttttcggc ttctgtcatt atttctgcat gatgaaacaa tgtctgaaca acagaagggt
     4441 taatcttttt taagatagga ataatctgat gtctgataaa atttcttgta taatcttctt
     4501 tctgattaga actgtcggta cgccattgaa ttttattctc ctttgcatat ttttctatct
     4561 catgcttttg tgcgaacaat attggtctga caatattttc tctttttacc tcaataccat
     4621 gccatgcagc cataccacca ccccgactca ggttaattaa aatggtctct gcaacatcat
     4681 cagcttggtg tgcagtagca ataaaatcat aatgaaaacg actacataac tcctgaaaaa
     4741 aattatatct taattcgcgt gcagtaacct gaatggattt ttgatattgt tctgacaact
     4801 tttcagtttc aaattcagtg gcatgaatca caattttatt ctccagacaa aatgaatgaa
     4861 caaattgtgc atcatcatcc gactcctgac cacgtaattt aaaattacaa tgtgccacag
     4921 ctattttgta tcctgattgc tgcagtagtt gcaataaaac cattgaatca atacctccac
     4981 ttacagctac aagaattgtc tgctccttgc tgaagagttt tttttctttt acaaactttt
     5041 gaaatcgtaa taacaagctt tgcttaattt tctgctaatg tatgctatcc taaaacatcc
     5101 atcaatgctt tggcttttaa catacactcc tcatactcgg cagccgggtc agactgaatg
     5161 gtaattgccc cacctgtttg tactgaaaca tagttgcgtt gagcattgta taagatggta
     5221 cgtatgataa cattgaagtc aaaatctcct gcaggatcta tataaccgac actaccggaa
     5281 aacaagcctc tgtttgattt ttcatgcgca tcaatcaatg tcatagcact tatttttggt
     5341 gcaccggtca tacttcccat aggaaatgct gcacgtatga tttctgaaaa actaatattg
     5401 ggctctacct cgcagcttac tgtagaaatc aattgatgaa ctgttttaaa agtatagaca
     5461 ccaagcaatt cattcacctc aactgtacct ggctttgcaa ttcgtgaaag gtcgttacgt
     5521 accaaatcaa ctatcataat attttctgaa cgctcttttt cgctgttctg cagattatct
     5581 ttaagccatt catcttcctc acgtgaagaa ctgcgaggtg ctgttccctt tattggttgc
     5641 gaatagattt tattttccct tctgcacata aaccgctcag ggcttgatga aattataaac
     5701 tgattttctt tcttataaaa acacgacatg ggagcacgtg tatttgcatt gagttttata
     5761 taagtctcga acgggttgat gtttgctgaa tcattataaa attcctgaca aaaagttacc
     5821 tcatatatat ttccctgttg tagttgctct tttaattttt ctacagtttg aatataatca
     5881 ctattgctga aacgtggtgt taatgccaca ttgccggatg catgcatttg tctaagctct
     5941 acatcatcag ccacaaactg ctttgttgtt tgctcaagct gatctatcat acgcagcttt
     6001 ccaccttcag aaactaaaac aacttgcggt acaaaaaaat gtaaaacagg aaaattcaaa
     6061 cggtcagtat gtgatgattt tagttgttcg atttcatttt ttagttcgta tgacataaaa
     6121 ccaaacatcc acttaccctt atttttctga atgaatgcct ccagtttctc taacgatttt
     6181 gatgtagctt caactgtatc cagagcatta atgccggcaa gaaaatcata ttttgtcaga
     6241 ccactgtagc tgccttctgc agcactgttg ctgtttaaaa aacaaaaatt acgaaaacca
     6301 tttgatgtct ttagcaactc ttcaaattcg gcaggtgaaa attttgtatc gctcataaag
     6361 caaaaaaaat caaaaaagat ttctattgct tatgattcta aaaaagtagt gaacacgttt
     6421 ttaacagata caataaaatg gaaggctttc tttcttttat acagggttaa ttaacctctg
     6481 tcaacaccca ttttactttc gggagctccg gtaattttat ttcttaacaa tgcaacatac
     6541 gacatgactt tagaattcat cagtagaata gctaaacaga aacaaaaaat aagaaatgtg
     6601 ataaaccgca gtttgcttag gggaagaacc aaaacaataa ataagatgtt gaaagctgcc
     6661 ataataaaag tacctccacg atgtccgaat tgctgacggc aaatcatgtg atgtaaatga
     6721 cttctgtctg ctttaaaagg tgaaataccg tttgatagtc tgataaaaac aactcttgtt
     6781 aagtcaaata tcagaataaa aattgttgca atggtcaagg aaggaccaaa agatactact
     6841 ggtgatgcta accaaatatt aaaaagtttt atagtacaaa tagcgataag aaagccaact
     6901 accaatgaac cggtgtcacc cataaatact cttgccggat taaaattaaa atacagaaaa
     6961 gacaacaagg cacctataac actgacacat aaaacagcat aagcctgttc cccgtgaata
     7021 tgaaacaata ccgtaaaaat gctgaatatg atgagtccaa ttgttgctgc taagccatct
     7081 aaaccatcaa taaaattgta ggcattcact aaaaataaaa taataaaaat actgatacca
     7141 tactgtgtca agatgggcag ttcatgtacg cctgcaaaac cattcaatgt ttgaatgcgt
     7201 aatcctgaat aagtgactat taaagctgct aagagttgaa tatttaattt aaccctggct
     7261 tttaaatcag taagatcatc aaaaaatccg gtaataaata aaatgagcat tgctgctgca
     7321 atagaatgaa agcttgtatc ctgactgttg tatccaaata aaaaaattga aatcattata
     7381 gctataaaaa ttcctatgcc ccctaatgaa ggaataatag ctgtatgatg tttgcgtgtg
     7441 tcagggcgat caaacaaatt ccacttggag caaagctgaa tccacgatgg aatgatgagt
     7501 gatgcaataa taagagaaga aataaatgga aaaaacagtt gaataatctg attcatacag
     7561 gtggtttatt taatcaataa tatatctgtt cgtgataatc tttctatttg tactccgtct
     7621 tcggtcttta acgcaggcat tttgtcttta acatcaacag taatgattct gtcagcatca
     7681 attcctttgc ctaccatata aactttaact gcttctgctc tttgttttcc aatattgcta
     7741 ctcttcttca attcaatttc cgaagcattt cctgaaacaa taatttttct gttgatattt
     7801 ttatataaat aatcaattgt agcttctatt acatcaaagc ggttgttgat atcatatttt
     7861 agtgaatagt cctgaaacac tacatagcct attgtttgat aattgtaatt agaaagttct
     7921 gtgttctctt ttattttact ctcacctttc attggcattt ctgcaagaga tacaactatt
     7981 ggattctgct caattattga cgggactata accggtgtag gtgtcagtgc ttgtggagga
     8041 gtaactcttt gctcaggtaa ttttttctta ttgacaggct caggttttct atcttcatta
     8101 cgtttacctt ttgcacgacc ttgtggtgtg taaacaagtg aaatttcata agctccacta
     8161 cctccaggct tgtacttcga tatattaaag tcatagcttg ttccaagaaa aactttatta
     8221 aactgatagc cggcataagc aaccatagca tcgttatttc tgtaaagcaa tcctaatttc
     8281 actaaactgt tattgcttaa tgacaattca gaaatcatgc ccgcactcat ttcctgaaag
     8341 ccatcttgtt tcataaaata aacaagaggt ttcagctcaa tagtttttga acaagtaatt
     8401 gatgcacctc catgaaaatt cattctgata ggatttacat tctgttcttc aataaatgat
     8461 tctttaggtt tgttgatgtg agaaaatgaa acacctgcaa agggttttat cagtatttct
     8521 ttattgccct gtgcataatg ataggcaatg ccaatattaa tatcaggacg tgtgactttt
     8581 gtattggcaa atgtttctcc attagatgct gaagggtcat aaccgatatc aggtgtgtac
     8641 tgactttcaa atgtaagttt gttggggtcg aaagatttct gaagaatgcc aacttggaaa
     8701 cctgatgata actgatgtct tttctgataa ccaaattttg catgatagct gatgccaccc
     8761 aatacactta atctgctgaa gccatcttta cctgcactgt ttttactaac cattaaacca
     8821 aaaccaacct tattgacaat tttatctaat gctattgttt gtgtcttgtt gcttaatccc
     8881 ggagcaagcc actgattgcg ataatcaact gctatgcgca tattctgaat accattaccc
     8941 gataaagccg gattataaaa tacaggtaca ttaaaatatt gtgatatgtg tgggtcttgt
     9001 gcattaacaa ttcccgacaa catgcaaaat gtagctatga tgattattct tttcatgtct
     9061 gccggttatt taatgattga aatatttcct ttcagcactt tatcactgcc atcattcaat
     9121 ttaatcttaa cgatgtaact ataagttcca ttgggtgcga gatttccctt tacagagcca
     9181 tcccatccta caactgcatt gtttgttctg aaaattaatt gtccccattg atcaagtatc
     9241 aacatgtctg ctgaaacaaa tcctgaacct tttaatctga agatatcgtt aatgccatca
     9301 ctgttaggtg taaatgcaga aggtaaaaac aagtcgtgtg caacggtaac tgaaatattt
     9361 tttgtaaaac tattctcgca gccaaactca tcttttactg taagtgttac actgtaatcg
     9421 ccttctgaag aataaatatg ttgtgggttt tcttcatttg atattgcatt gacagaacca
     9481 tcaccaaaat tccatgtata ggtgttattc cctgttggag tattgttttg aaatgaaaca
     9541 gcataaccac catctgcttt cacagcttga ctttctgcaa aatcaacatg taatataggg
     9601 tagatgtatg caagcacagg aaatcttgaa acacttgtac agccattaga attaagctgc
     9661 acataaaatg tagtgctctc tgcaagcggt gctgtagcat agctgctgcc tgttgcaagt
     9721 gctgtgcctc ctgctgcctc atcgaaccaa ctataggttg tattactttg ctgagtgatt
     9781 gttaacaaaa caccttcacc actgcatgat gaatcactgc ttgcttcaat tgatgaaggc
     9841 attgttttca aagaaacatt aaccggtggg gaaacagagg tacacccaaa actacttgtc
     9901 actgctacag tgtagtttgc agctgtcttg gcataaatgc tttgtgtggt ttttccatta
     9961 gaccataagt atgatgttcc tgctgtagca gttaatttta ccgagtcacc tgggcaaata
    10021 acctgtgctg tggcgggtga tattgctgca ctgaaagatc cgttaaggtt tatgatcacc
    10081 gaattgcttt gatagctaca acctccagta tatgttatac tcactgcgta agtgccgggc
    10141 agggtggcat tgattgatga tgttgttgct ccatttgtcc atagatatgc tattcccggt
    10201 gaagcagtta atgtagcact tccacccgaa cagattgaaa taggtcccgt aggagaaatt
    10261 actggtgtac ttgcatcaac aatagtaata cttaatgctg ttgttgttgc ataatttcct
    10321 aatggtcctg ttgcagtaca actgataaga tgtgtacctg ccgaaagtgt tcctaaagat
    10381 aatatccctg ttgtatttgt tgctaatact gtacttcctt ctttaaaggt atagctgctt
    10441 agcccttgtg gcgaagctgt aattacagaa gttgaacttg gacaaaacac actcacatta
    10501 tttaatgttg aaagtgttag tggattgctg ctctgattca tgttgatgag tcggatcaat
    10561 tcatttcctt catcaccaat aaaatatttg tctgatgaat gataagccat acatgatggc
    10621 gaattgaatg atgcctgaag tagcgggcca ttagccgaac ctgatgttcc tgctgttcct
    10681 gcaaaggttg ttactgtgcc attgctgatt ttacggatac agaaattatt tacatcacca
    10741 acaaaaatat cacctgatgc atctgcgcaa atgcctaaag ggttatcaaa tttactcact
    10801 gaagctgcac catcaacata tcctgcagta cttcctgcat aggtactcac tacacctgaa
    10861 ggagtaattt ttctgatttt attattccag gtatctgtaa caaataaatt tcctgactgt
    10921 ccaatagcta aactatgagg atgatcaaac tttgcattat ttcctgtgcc attagcatcg
    10981 ccactctgaa aagcagttcc tgcaaaagtt gtaacatttc cgttacttat cttacggata
    11041 acatgagtca taaactcact cacatatata ttacctgaag catctactgc aatacctgtt
    11101 ggaaatccaa actgtgcact gcttgcaggc ccatctgtaa ctccggatgt acccacccct
    11161 gcaattgttg taacagctgc tgttggcgta atctttctta ttaaataatt ttttgtgtcg
    11221 gctacataaa gatttccgga ttgatcaatt gctattcccc aaggttcaaa aaacgatgct
    11281 gcagctccta ttccatctga gcttcctgca cttcctgatc ctgcaaatgt ggatactaca
    11341 cctgcaggtg taatttttct gataaggtga ttatttctgt ctgcaacaaa taggtttccc
    11401 gcctgatcgc aaaccatacc atgtggattg ttgaacgatg ctgaagtacc tgagccattg
    11461 gcagcacccg aaacaccatt ctgccctgcc agtgtaacta cattctgagc attggctgtt
    11521 aacatgcata acatgacagc ccatattgct attccggctc ttatcattcg tttttcttca
    11581 aaagtgaact cccttttata ctttctcttt gaagtaaggt tacaccattg aaacgttaca
    11641 ccggaaaaaa ttacactttg aaaatgtgat taagagctct gaaacaactt tttacagaaa
    11701 attttaactg ttatcctgaa tcctgtatgt aaagttaaac tagtctgaaa tgannnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaatcac ttatactgat tttattttga
    11881 gcagggtttg ggtaaatcac taatgattgt ccggttagtt cgttaactga aactaattgt
    11941 tttaagtaaa caggtttaat tgcttcaagg ctatcaccca aattattctt catcaccaaa
    12001 cgcaaaaaat aatttcctga caccaatcca ttagtattcc aactacctag taatccattg
    12061 ttgtgaactt cagcaattac attagaaact attggtgccc aatttgaagt catgacgctc
    12121 caatacaaac tataattatc aaagtccatc acagcgcctg atggcccctg atcaatccat
    12181 gctgatccat taatcggaac aatggtattg attattgctg tatcaggtga tgaaatattt
    12241 tgcatccatg ctactgctcc atcatacggc acagggtctt gccctaaagt actttgaaca
    12301 caaaccatca aactattgct taatgcagtt ggtgccataa aaggttgcca accatatgcg
    12361 agaataaaaa ttcctttttt ctcacttctt gccattgtat aaatagttgt tcctaaagca
    12421 agaataccac tgctgtctgt tgcccaaaaa tatccgccac ttccatcaag caataagttg
    12481 ttcgcattta caaatgcact ttgttgtgta cccacttcgc ctaactgaca actatctata
    12541 tcaacctgtg atgaatcaaa aacataaaaa ctccatgtct gtacattagt gtttatcaaa
    12601 tgtaggtttc tgtctgctaa tggtgctgta taattgctat aatttgaatt attgaaaata
    12661 ccagagacag aagctacatc acctcgttca aaccaacaac caattaaacg caggtttgaa
    12721 tttgatatag taacatcaga accattaaca ggcatcaaag cccacattac attatgacaa
    12781 ctgtctgcac taacctgata tcctattcca cttattcccg gagtgctgtt gttgcataca
    12841 taattgtaaa ccgtatcacc gggaggaaag gaatagttta ctacagcagt atccggaaaa
    12901 tggtgccaga gaattaaagt atctgcattc ttaaaagttg cctgacattt atctgttaaa
    12961 atatattcac ctgttatgtt acaaccattg atgttaatat tagcattacc atacaaacca
    13021 catgtggtaa aatcattttg atgaatattt gtaaattcaa ctactgcaga gtcggctaaa
    13081 tataaattat gtgacatgcc gctatagtta aaagatgaat tctttatctg aacaaaacca
    13141 ttttgtgcta caataaggct tcgctcataa atatattgtt gcggaaaagt aaatagtgtg
    13201 gagtctgcaa aaagttttcc attaccaaaa acataaattt cacctgtatc aacaactgtt
    13261 gcctgattaa aaattaaaac cccgttatta aaaaccaaaa tggggcctgt atgtgtaaag
    13321 tttcctgtaa tcactaaagt atcgtttatc tgtgcgccta ctattatggt atcagctacc
    13381 ataaggtctt gttcattatt atcaaacact tttttgaaaa gaaccgatgc aggtgatgaa
    13441 acaaaaccgg ttggtgattg acttgcgtgc ttgttctgat tgctccattg tgctgaggca
    13501 ataaatttat ttaatgcttc attgattttt tcctgaggga ataatcttgt tgcattactc
    13561 tgtgaaagta tattctgatt tattgaaatc aataagaata ctaaaatgta aattatgcga
    13621 tttaccattg gttgatgttt ttaaacgtat cataacttga gttttgcaat aaaactcagc
    13681 ataatagttt ctgttattta tcaactgtaa aaataacaac agtattcatt cggcttcaaa
    13741 aaaaaataaa aattaaggta aacctacttt tgtggtaccg gctgctcttc tataatttgc
    13801 tgccttaccc aattttttga ctttgcattc caccaccatc tgttattcag attttgttct
    13861 gttgactcct gtggaaactg caattctttt ttccattgtg ggttattgtt ttcatcaaaa
    13921 ttaatttctg tttcatgcca atacatgctt gcatcaggat ttatagaaat ttttgcctgt
    13981 gaacatgcac catttttatg aaaccttttg agttctgttg tagaagaaaa acttcttctg
    14041 atatcgttta tagtataagt tttattaccg aacggatcga aaaaaatcag ttcacgttta
    14101 ccatcatgcc agggtgttat ttctactgat accctcttag gtgttgattg ataataatag
    14161 aaaacagatg tttcagctct tgtgctttta tctgtttgct ttgttgaagg agtggtaatt
    14221 tgtatttgct tcttcgattt taatgtatct tgaatattct taggctcgac tgcgtgcaaa
    14281 atcttttttg atactgtctg atgggttaaa gaagtatcca tttctttctg ctcatcattc
    14341 ttaatagtgt cctgattatt ttgtcctgca atcttttgct ctaatgtttg taaactcttc
    14401 tgcagcgaat caattttttg gactaatttt tcatcagaat tatttacaca tcctgctaaa
    14461 aaaataagta aaatatttaa agttaaaagt cgtaccatat agtttaaagg atgaaagtac
    14521 ttcaattttt ccataaaaaa caaaagccgc attgctgcgg ctcctgtctt tagaaaaatg
    14581 aaactgatta atctttttta tctaacatca gaatttcatt aatcactact tcggttatgt
    14641 acttcttgtt accttctttg tctgtgtaac ttcttgaagt caacttacct tctacagcaa
    14701 cttctttgcc tttgtccaaa aacttctctg caatttctgc tgtcttaccc catgccacca
    14761 gattgtgcca gttggtttct gttaccttgt cacctttggc attcacatag gtttcgtttg
    14821 tagcaagatt aatacgcgct actttgttgc cgttgtcaag attttttact tctggtgctg
    14881 ctccaagatt tccgatgagt cttacgctgt ttcttaatgt gttcattgta ctttgttttt
    14941 aattgtttat attatttatt ttgtttgaat cgtttgttgt ttccgattga cgatgcaaaa
    15001 gtgcagcagc cccggattct aagtcggatg ataagcagtt agttccgaaa acaatcgaat
    15061 gtaaccgttt gtaatcggat tgttttatct ttgtattctt caaattatac tctgaaaatg
    15121 aaaaatgaaa agacctgttt agaatgtggt gaaacattgg ttggccgtgc cgataaaaag
    15181 ttttgtgatg atcaatgccg cagcaattac aacaacaaag ccaatagtga tgtaactgca
    15241 gaagtgcgaa acatcaataa tattttaaga aaaaacagac gcattttgga atcagttatt
    15301 ggtgctgatg gaaaagtaaa aatcagtaag caaaaattat cggataaagg atttaatttt
    15361 aagtatcaca cacatcagca tcagacccta aaagggagtg tatataaatt gtgttatgat
    15421 tacggctatt taccaataga aaatgatttg atttatgtta taaaatgggg aaactttaca
    15481 ccacaacaag atggaaaata attgttatgt ttttatctta aagaacttta taaagtgaat
    15541 aaataataag aggctgcttc gaattatgaa acagcctctt attacttaat attaataaat
    15601 atcattccac tactatactc atctgattgt tgctgccatt ggtttgtaca ttcagcaagt
    15661 acactcctct ggccactttg ctcaaatcaa gttgtaagga atttttacct gtaacattcg
    15721 catgtgtctg tctgattaat acacgaccgc tgatttctga aagcgttatt agtgtcagtc
    15781 ctttttctgc tgtgtattct acgtttaaca tatctgatgt tggttgtgga taagcattga
    15841 gaatattgac ttttgattga aagttgttct gaaagcctac agtcgggcct atgttaatca
    15901 tataatcttc tgtctctcct aaactatacg atccgcaagg tattattgcc gatgctactg
    15961 ctgtttcttg tgttatcaca cgcattgctg ttaatccgga aacggccgac atcggtatta
    16021 aaatggttcc gctttctgtt cgtggccccg aagtgtagga tggtgaaaca tatacttctt
    16081 ctccaggatc actgaacaat ccattctgat tataatcaat aaatattttt gacatgtaga
    16141 agtagtagct tgcacaagaa tttatctgaa tagagaaagg aactaaagtt ccacgtagta
    16201 cactcggtgg tgtcggtgcg cctgctccac tcatataatt actgtatctg tttttaattg
    16261 atcccacccc cgatgcagtt gtattacaat cagaagtatt atttaatgta ccaaatgtca
    16321 cattgaaaat atcttcatcc gctgcagaat ttgcactaga tgcacagtag caggaggtaa
    16381 ataaattcat taaaacctga atcggtgctg atgtatcact ctggctgcta ttggtacatg
    16441 tcactataca ttgataccag gtattagtca tttgtgtctg gttctgtatc ggatagttgg
    16501 taccaaaatt agtccatgca ataccatcag gtgatgattg ccattgatat gtcaatccgc
    16561 taacaggagg attattttgt aacgatagta caactgcaga atcggcacat acagaaacag
    16621 cacttgcttc tgtattgccc ggtgatggtg taccactaca tggtgttgct ccggtaaact
    16681 caaactgtat ttgtgctaaa cccatatttg aggtgtttct gccgttggct accggtggtg
    16741 ctaaggggtc gggatttgta gtatcattgt aatagtaaat gcccttattt gctgttgttg
    16801 tatagcctgc ccatgctgga gtattacccc aattaggtgt gttttcatca actgcaatta
    16861 ctaaattaga tattccatcc cagagaaagg gcgttggtaa agttagttcc atccaaacat
    16921 tggctgtaac attagcaggt atcgtaccgg caaaaacctg cgtcattgaa gcaactggaa
    16981 tccaatcggt agttgatgag aacgaggttt ttgatgtatt tccaaaatac actacccaat
    17041 cttgccataa tacagtagga gcagacatag taggtttaaa tctaatttta gagattaatg
    17101 ccgcaccgcc tccccagccg ccactattaa tctctgtcgc ttcaactatt tgctgcgaat
    17161 aggagtatcc atatagatag taattgggcc aatagttact tgttcccgta ccggcactac
    17221 cgattgacac tgtaatttgt gcttgtgctc ctgtaaagac tagcgttaaa agaactgtta
    17281 agcttagtga tacatttaca ataaattttc tttttgcagt aaaaatctta gttatcataa
    17341 tttttaagtt taaatttcct atgattttaa aagagctttt agtgtttaga gatattgtat
    17401 tgcttattgc accaatcaga cttcaatatt aacaattatt tattgattac tcaaatttac
    17461 cctaacatga acgaggtttt gttttcagat attcatcgtt tatagctcat gattgtttag
    17521 ctcaatttgt ctatcttctc ctaaatctgt tttctaaaaa ggttttactt aaaatgatat
    17581 aaaaacaaaa caggctacct tttcaggtag cctgtttgca attaataata actaaaatta
    17641 cttatctcat actgtattac tccactacca ctctcacctg attgttgctg ccattggttt
    17701 gtacattcag ca
//
LOCUS       DAOQNP010000068        17328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1162_length_17328_cov_11.187460, whole genome shotgun
            sequence.
ACCESSION   DAOQNP010000068 DAOQNP010000000
VERSION     DAOQNP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17328
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_17328_cov_11.187460"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..575
                     /locus_tag="PKY09_09555"
     CDS             120..575
                     /locus_tag="PKY09_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB13144.1"
                     /translation="MNHWVETAIQLLTKSLHPIPQELNEIDWKSGLSDKSERLAQHIS
                     AFTNYPNGGYLAFGIGNNGSPMPLGKNEMDTVVQKLGNIARNNLAQPVGIEHAVTDYK
                     GYPVLFIRIPEHTDKPVHLRGSDIFDSYKRSAGQTVRLSRHEVKQLIAI"
     gene            695..1567
                     /locus_tag="PKY09_09560"
     CDS             695..1567
                     /locus_tag="PKY09_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB13145.1"
                     /translation="MSILQTLAGEDLIKKSTKGWGILNLGAILFAKDLKSFKELKRKA
                     VRVIVYKETSRINALKEQEGGRGYASGFEGLVNYIMDQLPSNEVIENALRKKVKVYPE
                     KAIREFVANALIHQDFLVTGSGIMIEIFTDRIEITNPGVPLVDTNRFIDTAPKSRNES
                     LASLMRRLNICEERGSGIDRAIEAIEVFQLPAPKFIRGDDYTRVILYAPTPLTRMNND
                     DRIRACYQHTCLHYVNNQPVNNQSVRKRFNIAKNNVSFASKIISDTLEAGLIKASDPD
                     SASKKFASYVPFWT"
     gene            2143..2751
                     /locus_tag="PKY09_09565"
     CDS             2143..2751
                     /locus_tag="PKY09_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13146.1"
                     /translation="MRDQIITLAEENRKVFGYSEKQIITSTKKHSSFDSLYAAMNKSS
                     MLEVVSVVPMESLQEISFNEKEDPFTITYDKNGASKKDSFLLEDVNNREAIVADLASI
                     KNFKRNTTVESKTKPLAFNLVVLAVVVICTWGARNIALDAEAGVQFEASGKRKALQNM
                     VADAIGTIGPTWSMLIGAIGLVYMIYRTYSRYQNPATIIKYY"
     gene            3804..4814
                     /locus_tag="PKY09_09570"
     CDS             3804..4814
                     /locus_tag="PKY09_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016792313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNB13147.1"
                     /translation="MNDLTVSAIDRQNILNNKTALENIQGYLGITGMMFQGEFRFTRQ
                     QIADFYGIDNSTIDRYLSQNEDELKHNGYINLKGKALKEFKEEFGWMLKEGAKAPQLG
                     IFNFRSFLNLGMLLAESEKAKALRSKILDIVIDTLNQKLGGSTKFINQREEDFLIAMA
                     REPVYRKEFTEALNKYLEMGNYKYAVYTDAIYKAIFLEKSSEYKVILKLEEKENPRDT
                     MYSEVLRLIASFEVGIADAMKSEYERLGRKIQPHEMNELIEKFASQRMWIPQIEDARS
                     KMASRDYGLRNIIHERLKPYINSLSAADYDRFLGDKSKDLLQRVIENPELLEVFKRLK
                     DR"
     gene            4815..5258
                     /locus_tag="PKY09_09575"
     CDS             4815..5258
                     /locus_tag="PKY09_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013754804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNB13148.1"
                     /translation="MAFAYFDTTHAIEVHDEIINQSGGVLGILNISLLESVIEHVQND
                     LYYPDLEHKLTHLFYSINKNHSFQDGNKRASIALSAYFLEINNCSFRVERFIEEMENI
                     AVDVADNWIDKDLLFEIITSIIYEDDYTEELKLKIIYAKGFNIAD"
     gene            5895..6719
                     /locus_tag="PKY09_09580"
     CDS             5895..6719
                     /locus_tag="PKY09_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNB13149.1"
                     /translation="MQRKVLFFMFLFLQVKLFAQSDSLVLRDLTLDNTAPAGMTELII
                     PSGTSLLAGFMYRANGSQKHPTMLLLHGLPGNERNLDIAQFVRSKGWNVLYFDYRGSW
                     GSQGTFSFKNCVDDVINVVEFCKKYQDSLKIDTSRIVLFGHSMGAWVCIKALEQLPTV
                     KKGFALSTWNIGTYYNSISPKTGRNLFMLNANLEDLYNSVDQNKVKYNLINDNFESFR
                     GKQIFMIDEHKFNSKLAETIKKSNPSSFEYLVWQTDHSFTNKRVALMKRLVEFLNR"
     gene            6997..8202
                     /locus_tag="PKY09_09585"
     CDS             6997..8202
                     /locus_tag="PKY09_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008754326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB13150.1"
                     /translation="MITRETYLQKLRQLKDQNIIKVVTGIRRSGKSTLLEAFKNELTA
                     NGISPRSIIFLNFEERENLHLTDWTVLYDEIIIKIKANKKYYVFLDEVQLVNDFEKLI
                     NSLFTKKNIDLYVTGSNAYLLSSELATLLTGRYIAINVQPYSFKEYALAYKEEKNIDR
                     LFRKYINASSFPEAVTLSQSNESLANDYLQSIYDTVVIKDIAQRYKLRNIHNLHRVIS
                     FVFDSIGSFISPTNIAAELNKNSQKKISHNTIIKYLDFLTNSYILYPASRFDIKGKEL
                     LTTNEKYYFVDLGLRNLTATNKYDTDLGHKLENVVYFELLRRGGKVYAGKNNDKEIDF
                     VVQRPNNEREYYQVAFTLNDEKTHKREVSAFSNIKDNYPKYILTLDFDNTNIDGIRKL
                     NVIDWLLNE"
     gene            8558..9052
                     /locus_tag="PKY09_09590"
     CDS             8558..9052
                     /locus_tag="PKY09_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HNB13151.1"
                     /translation="MEKIEVAFKVTLDDVKEFQFYHYKKSISYKFFIVCCIFFVPLTL
                     LIFFADPKQNWLLGCLGFIYLFLLIFWIPMRIKRRSRLNFTTTKAYEDEINWTIDEIG
                     FVVKGTNFEGKNQWTSLYKVKELKNIFVFYSNKFAATILPKRALTERNMAELKKFFNL
                     KSPK"
     gene            9938..10846
                     /locus_tag="PKY09_09595"
     CDS             9938..10846
                     /locus_tag="PKY09_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB13152.1"
                     /translation="MKIYSKSQFKDETADRNYFENWVSRLEKNNSRQYERYEIETSLG
                     MTHIWGLNTRDEQLDTLVIFPGARTTSLIWDFDKGLDNLNHKMKIFMVETNGLPNLSE
                     GMTPDIKSLDYGLWATEVFDKLNIDKAFVAGASFGGLICMKLGIVKPERIKAAFLLNP
                     GCLQPFSLTLKNLYYNLLPIIKPNERNVLKFLDTAVFSKPNHKLSGFSEKMLVDYEVF
                     AISRYRDKTQKPYYMGKQLEDVKVETYLLVGDKDLLFPYQKSVDNAKRQIKTLKETKI
                     YNNVGHGIETYDKALNYIGEKIKNCH"
     gene            11495..11821
                     /locus_tag="PKY09_09600"
     CDS             11495..11821
                     /locus_tag="PKY09_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNB13153.1"
                     /translation="MNYYLKVLQNYATFSGRARRSEYWYFFLINVIISFILGFVGGVM
                     GTSILSNIYVLAVLIPGIAVGVRRMHDVDKSGWFLLIPIYNLILACTEGTKGENEYGA
                     DPKEEA"
     gene            13314..13604
                     /locus_tag="PKY09_09605"
     CDS             13314..13604
                     /locus_tag="PKY09_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB13154.1"
                     /translation="MNSFGEYLRSRREQLGLPLRKVAAELDIDTSILSKIERSERVAT
                     KEMLSTLAKTLDVQEKEMEIEFIKTFILSYLGELKFLKSGLEETLNTIKSRN"
     gene            13607..16852
                     /locus_tag="PKY09_09610"
     CDS             13607..16852
                     /locus_tag="PKY09_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNB13155.1"
                     /translation="MSLDNKIDQTDLTFFTNEEGHTLLSRFKSTLKDTQLFDVLVGYF
                     RASGFHQLYDALEPIEKIRILVGLNVDRDSYEIMQYHEQSSTIDFESHQRTKKRYQQN
                     LKEEIENSHENDNLLEIGIRKFIEFLNTDCQDPAMDKAYKGNGKKLEIRAYPTKNIHA
                     KVYIGKFKPEDRDYGFVITGSSNFSESGFVANREFNVELRNKSDVLFAENQFNQLWRE
                     SVDISEDFVDTIQNKTWLNDQIKPYELYLKLIYEYLEEDINLADEFEPFLPEGFMKLK
                     YQNQASIQAKKILERYNGVFLADVVGLGKTFITALLLQQLQGRTLVICPPVLIEYWKD
                     SLFDFGIRSFKVESLGKLEHIIKNGLERYDYIVVDEAHRFRNENTQSYANLLDICRGK
                     KVILVTATPLNNTVDDIFAQLKLFQAPKNATIPGIPNLEKYFASFRTRLAKLEKTDPE
                     YKKLIKNISDDIRNSILRYVMVRRTRTDVMTYFKQDMKLQGLTFPNLDNPQKIVYKYE
                     GNLEIVFNHTIKKLQEFTYARYTPLLFYVGNKALSEFEMQQQRNVGGFMKGILVKRLE
                     SSFYAFRQSVDRFITSYEKFIAMYQNGTVYISKKVDVYELIESDNFERLEKFVEEENV
                     QKFDSKDFKKEFIDKLEFDLSILKEVKNLWQNVNSDPKLEMFIHELKTKTSLKNNKLV
                     IFTESKETGDYIYDALQDEFSGKVMFYSSTGGRHTDKNLTSNHTISRDIITANFDPKY
                     KEKRDDLKILITTDVLAEGINLHRSNVLINYDLPWNPTRVLQRAGRVNRLGSNFSTVY
                     IFNFFPTTQSDEHLGLEINITNKIQMFHDILGEDAKYLSDGEEFGSQELFNTLNSKTA
                     YTGEDVEGDSELKYLEMMRKIRDEQPDLFEKIRNLPKKARSGFKKEKLDDDALVTFFR
                     IGMLKKFYINLGDKSNEITFFDAVREMECSPDTKRENIPNTYYHMLQTNKARFELDTT
                     DGDEPTKGSGGRSNLKYIETRLKEKSFKNFKGFTESNDEFLNGVRGMLANGTIAKKTT
                     QVIKADLEKTTDPLQILHILEKHIRFVAIEREQISKKFQNREVILSGYLVK"
     gene            16891..17274
                     /locus_tag="PKY09_09615"
     CDS             16891..17274
                     /locus_tag="PKY09_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNB13156.1"
                     /translation="MEYDTYKHTDITEKIIKAFYKVYNTLGYGFLEKVYENALFIELQ
                     SMGIFVEKQRRIKVFYLEHEVGDYFADLIVDNKVIVELKASEALCEEHEYQLINYLKA
                     TEIEVGLLLNFGKRPEFKRKIFSNE"
BASE COUNT         6091 a         2980 c         3352 g         4905 t
ORIGIN      
        1 tgttctttga ccgtttttta acataatttg ttgaataatg cgcaacttgg gttaagtgta
       61 acagacagat actccataat tcaatgtgtt acagaagata aaaaatgtaa atttgtgaaa
      121 tgaatcattg ggttgaaaca gcaatacaat tattaaccaa aagcctccat ccaattccgc
      181 aagaactgaa tgaaattgac tggaaaagtg ggttgtctga caaaagcgaa cgattagctc
      241 aacatatttc tgcatttaca aattatccaa atggtggcta cttggctttt ggtatcggaa
      301 acaatggttc tccaatgcct ttgggcaaaa atgaaatgga cactgtagtt caaaaattag
      361 gaaacatagc gagaaacaat ctcgctcaac cagttggaat tgaacatgca gtaacagatt
      421 ataaaggtta ccctgtttta tttattcgta ttcctgaaca tactgataaa ccagtacatt
      481 taagaggtag cgacattttc gacagctata aacgtagtgc aggtcaaaca gtaagattgt
      541 cacgacatga agtaaaacaa ctcattgcaa tttaaccggg tttcgatttt gaggcacaag
      601 tcgctatgca taatttagac gatgacgatg tgttgaaatt aatggattat gacagctatt
      661 ttactttaca agataaaaga cttcccgaca ctaattgagt attttgcaaa cgttggctgg
      721 agaagattta attaagaaat ccacaaaagg atggggtatt ttaaatttag gagccatatt
      781 atttgcgaaa gatttaaaga gtttcaaaga gcttaaacgt aaagccgttc gggtaattgt
      841 ttataaagaa accagcagga ttaatgcatt aaaagagcaa gaaggcggta gaggttatgc
      901 ttcgggtttt gaagggttag taaattacat tatggaccaa ctcccgtcaa acgaagtgat
      961 tgaaaatgct ttacgtaaaa aagtaaaagt atatcccgaa aaagccattc gtgagtttgt
     1021 tgcaaatgct ttaattcatc aagatttttt ggttacgggt tctggtataa tgattgagat
     1081 ttttacagat agaattgaaa taacaaatcc tggagttcct ttagttgaca ctaaccgctt
     1141 tatagacact gcaccaaaat caagaaatga aagtttagcg tcacttatga gaagattaaa
     1201 tatttgtgaa gaaagaggaa gtgggattga cagagccata gaagccatag aagtttttca
     1261 attgcctgct cccaaattta taagaggcga tgactataca cgagtaatct tatacgctcc
     1321 tactccgtta actcgaatga ataacgatga cagaatacga gcctgttacc agcatacttg
     1381 tttacactat gtaaacaatc aacctgttaa taatcaatca gtccgaaagc gttttaatat
     1441 tgcaaaaaat aatgtgtcgt ttgcctcaaa aataatttcc gacactttag aagcaggatt
     1501 aatcaaagct tcagaccctg acagtgcttc aaagaaattt gcatcgtatg tgcctttttg
     1561 gacttaattt aatttgttta caaaatcaac ccaaagacaa aaacaaaccc aaacggctga
     1621 tttacaagca cttgcttatt tcattggaaa atcccgacac caatgttaga aagaacaccg
     1681 acagccaaga ttaacccgaa tatacaaaag ccctccataa aacttccaat ttaatcaata
     1741 agcaagccga gcatttgaat atacggtgta cttcaacatg ctcagaataa catacagcga
     1801 cacagtatga tggactgtat ttctcttagg tgaattccta actttattgt cggatggatg
     1861 gctgactttt gtcggtcaaa gaaagaagaa aggacttatc tttcccaatg gaaagtacgg
     1921 cagtgtcacc aagcatggag ggctcattgt accgcaatcc cactccccgg ggtggctcac
     1981 tttacaccag aatactcatt ttactcaaac ccaataatat aatcctattt aagtataaaa
     2041 tgatcggcat ctccagataa attaccatta tctaaatgaa agccgtatat ttatcagcaa
     2101 aattaaaaca taggaaatct taaattcgaa caaactatac ttatgaggga ccaaattatt
     2161 acacttgcag aagaaaatag aaaagtattt ggctattcag aaaagcaaat tattacaagt
     2221 acaaagaagc acagctcttt cgattctcta tatgcagcga tgaataaatc aagcatgttg
     2281 gaagttgtaa gtgttgttcc aatggaatcc ctgcaagaga tttcttttaa tgaaaaggaa
     2341 gatcctttca cgataacgta tgataaaaac ggggcatcaa aaaaggactc cttcttgttg
     2401 gaagatgtta ataacaggga agccatcgtt gctgacttag cttccattaa gaattttaaa
     2461 cgaaatacaa cagttgaatc aaaaactaag ccactggcat tcaatttggt agtcctggca
     2521 gttgtagtta tatgtacctg gggagccagg aatatagcat tagatgcaga agcaggcgtt
     2581 caatttgaag cctcaggcaa aagaaaagca ctacaaaata tggtcgctga tgcaattgga
     2641 actattgggc ccacatggag catgttaata ggtgcaatcg ggctagtcta tatgatttac
     2701 agaacctaca gcagatacca gaatcctgca actataataa agtattatta aataccattt
     2761 tagaatcctt aagtattggt tagacaccat atttgaatac attagatttg aatcaggtaa
     2821 aaatcgctaa caaataaggt ttaacctcca gaaacgaagc ggaaatgcga tctaaacctc
     2881 gggttatagt cctgacctcc atggattgaa gggatgtttt gggaacgatc cacaacatgc
     2941 tatgggtaca caataagaaa aggcgggctt atggaagact ttttagtggg gtggtttttg
     3001 caatttacgg tggaagcaat gaagcaatca cctaaacctt gaataccgtg tgaatggtaa
     3061 gcgaccaagt gattgacctt aatttttttt gatatatccg gggagaatcc ctctaacctg
     3121 cttttaaagt gagatggaat tgtatagagc attttcccgc accatccata gggattgata
     3181 tttctatccc tatattaagg aataaaactt tttcaagaac gggcttttca taactgatgc
     3241 accatttttt cgtacttcgt caagggattt actatactga acaggcgtag taaggaatgt
     3301 cttaaaaaat gtaacaggcc gagcaactac acgtcatttt tctgtatata caaaagccct
     3361 ccaaaaatct tccaaattaa gccactaagc cagcccggct tttgaatatt ttccattcag
     3421 caagaccggt tgatggactg tatttctctt aggtggattc ctggcttttt tgccggatgg
     3481 atggctcact ttactccgga atatccaact gagatttaca cgaatgtaac ggataagctg
     3541 agaaagaaga taaaaagccc gtttgataga ttggaaatct gagtgaaagc ttacttttat
     3601 acggaaatat acgccataag gcgtatagag agatgttggc ggcaagtgta taacaacaga
     3661 cggaacaatg acaatacgtt ggcaaattaa gacaggatga ggaaaacttt aagacagata
     3721 aacgcattga ataacaatga cttacatatt gtttccctta tttgtgaggg aaacatggaa
     3781 agctttcaac aatatttgca aatatgaatg atttgacagt atcggcaatt gaccgacaaa
     3841 atatacttaa caataaaact gcattagaaa atatccaagg atatttaggg ataacaggca
     3901 tgatgtttca gggtgaattt aggtttacca gacagcagat agcagacttt tatggtatag
     3961 acaattcaac tatagaccgt tacctatcgc aaaatgagga tgagttaaag cataatggct
     4021 acataaatct caaaggcaaa gctttaaaag aatttaaaga agaatttggc tggatgttaa
     4081 aagaaggagc caaagctccg caacttggga ttttcaactt taggtcattt ttaaacctag
     4141 ggatgcttct cgcggaaagt gaaaaggcaa aagcacttag gagtaaaatt ctagatatag
     4201 ttattgacac gcttaatcaa aaattaggtg gatcgaccaa gtttattaac cagcgagaag
     4261 aagacttctt gatagcgatg gctcgtgaac ctgtatatcg caaagaattt acagaagctt
     4321 tgaataagta tttagaaatg ggtaattata aatacgctgt ttacactgat gctatctaca
     4381 aagcaatatt tcttgaaaaa tcttcggaat acaaggtgat tttgaagcta gaagaaaagg
     4441 aaaatccaag agacacaatg tattcagaag tcttaagatt aatagcctct tttgaagttg
     4501 gtattgcgga tgcaatgaaa agcgagtatg aaagattagg acgaaaaata caaccacatg
     4561 aaatgaatga gttgatagag aaatttgcat ctcaaagaat gtggattcct caaattgaag
     4621 atgcaagaag taaaatggca agccgagact acgggcttag aaacatcata cacgaaagat
     4681 taaagccata tattaattca ttaagtgctg ctgactatga cagattttta ggagataaga
     4741 gtaaggattt attacaaaga gtaattgaaa atcccgaatt gcttgaagtg tttaaaagat
     4801 taaaggacag ataaatggca tttgcgtatt tcgatactac tcatgctatt gaagtgcatg
     4861 acgagataat aaaccaatca ggcggtgtgt tagggatttt gaatattagc ctattggaaa
     4921 gtgttatcga acacgttcag aatgacctgt actatccgga tttagagcat aagttaacac
     4981 atttgtttta ttctatcaat aaaaatcata gctttcaaga cggaaacaaa agagcatcaa
     5041 ttgcattatc agcatacttc cttgagataa ataactgctc ttttagagtt gaacgtttca
     5101 tagaggaaat ggaaaatatt gctgtggatg tggctgacaa ttggatagac aaagacttgc
     5161 tattcgaaat aattacctca attatttatg aagacgatta tacagaggaa ttaaaattga
     5221 agataattta tgcaaaaggg ttcaacattg cggactaatc agttttatat ctgacagaaa
     5281 agaacaccaa ccaccaacat taatccgaat atacaaaagc ccgtcaaaaa tcttccaact
     5341 taagtcacta aaccagcctg gcttttgaat atttggtgca cttcgacaag ctcagtatgc
     5401 cattcagcga gaccggatga tagactgtat ttttgttagg tggattctta tcttttttgc
     5461 cgtagggatg gctgactttt gccggtcaaa gcaggaagat ataacttgtc ttctccaaag
     5521 ggaaacatgg ctgtgccacc ctacatggag ggcactatgc tcctggatca atggctcaca
     5581 ttacaccgga atatccaata ttaaaagact ccatgtaaat catattgccc aaacccaaca
     5641 attaaatcct gtttgagtat aaaatgatcg gcatctccgg ataaagtacc ataatctaaa
     5701 tgaaaacctt atatttatca tcaaaatcaa gacacgggaa aattgattcc ccacatagga
     5761 atgaccgggc atgtccaact tgaaatattg atacttgatt tgaatcactt tttagggttg
     5821 aaaagggtta agtatcaata ttcctttgag ttgtacgcta ctcataagat acatattcaa
     5881 caaaaaagat aactatgcaa agaaaagttt tatttttcat gtttctgttc ttacaggtaa
     5941 aattattcgc ccaatcagac agtcttgttt tgagagattt aacactcgac aacactgcac
     6001 ctgctggcat gacagaattg attattccat cagggacttc attactagcc gggttcatgt
     6061 acagagctaa tggttcacaa aagcatccta caatgttgct tttgcatggt ctgcctggaa
     6121 acgaacgcaa tcttgacatt gcgcagtttg ttaggagtaa gggctggaat gttctttact
     6181 ttgactatcg gggatcatgg ggcagccagg gaacatttag cttcaagaac tgtgttgatg
     6241 atgtaattaa tgtagtggaa ttttgcaaaa agtatcaaga ctctctaaaa attgacactt
     6301 caaggattgt gttgtttgga catagtatgg gcgcttgggt ctgcatcaaa gctcttgaac
     6361 aattgccaac agttaagaaa gggtttgcgt tgtcaacatg gaatatcggg acatattata
     6421 attcaatttc gcctaaaaca ggtcgtaatc tcttcatgtt aaatgctaat cttgaagact
     6481 tgtataattc cgttgaccaa aataaagtta aatacaacct aatcaacgac aattttgaat
     6541 catttagagg gaagcagatt tttatgatag atgaacacaa gttcaacagc aaattagcag
     6601 aaactatcaa aaagtcaaat ccgtcgtcct ttgagtattt ggtatggcaa acagaccatt
     6661 catttaccaa taaaagagtt gcactaatga aaagacttgt ggaatttttg aaccggtaag
     6721 caacgtacaa cattaatccg catatgcaaa ggccagccaa aaatcttcca aattaaatca
     6781 ctaaacaagc cggacttttg aatattcggt gttcagcgag acccgattat ggactgtatt
     6841 tctcttaggt gtattcctgt ctttttaacc ggatggatgg ctgacttttg tcagtctaag
     6901 caggaagata taacttgtct ttttagctat tgacgaaaaa tgtatatatt ttggattttc
     6961 gtcaataatt attatcttta cacaaagtaa aaggcaatga taacacggga aacttacctg
     7021 caaaagctcc gtcaactcaa agatcaaaac atcatcaagg ttgttacagg tattagacgt
     7081 agcggcaaat ctacgctttt ggaagccttt aaaaacgaat tgaccgcaaa cggaatttcg
     7141 cctcgcagta tcatttttct gaattttgaa gaaagagaaa acctgcattt aacggactgg
     7201 acagtacttt atgacgaaat catcataaaa ataaaagcaa ataaaaagta ctacgttttt
     7261 ttggacgaag tgcagttggt caacgatttt gaaaagctta tcaatagcct gtttactaaa
     7321 aagaacattg acctatatgt tacaggttca aacgcttatt tgctgagcag cgaattggca
     7381 acgcttctga caggaagata catcgctatc aatgtgcaac cctattcatt caaagaatat
     7441 gctttggcct acaaagaaga aaaaaacatt gaccgtcttt tccgtaaata cattaatgca
     7501 agcagttttc ccgaagctgt aacactttcc caaagcaacg aaagtctggc aaacgattac
     7561 ctgcaatcca tttacgatac agttgttatc aaagacattg ctcaacgcta caaacttcgg
     7621 aatatccata acctgcatcg cgttatcagc tttgtatttg acagcatcgg ttcgtttatt
     7681 tcgcctacaa acattgcagc ggaactcaac aaaaattcac agaaaaaaat atcccacaac
     7741 accattatca agtacctgga ttttttgacc aattcctata ttctttatcc tgcttcacgt
     7801 tttgatatta aggggaaaga attgctgaca accaatgaaa aatattattt tgtggacttg
     7861 ggattgagaa acttaacagc aactaataaa tacgataccg atttaggaca taaacttgaa
     7921 aatgtagtgt atttcgagct tttaagacgt ggcggaaaag tgtatgccgg taaaaacaac
     7981 gataaagaaa ttgattttgt agtacaaaga cccaataacg aaagagaata ttatcaggtt
     8041 gcttttactc ttaatgacga aaaaacgcac aaaagagaag tttcggcatt tagtaatatc
     8101 aaagacaact accctaaata tattttaacg cttgattttg acaatacaaa catagacggt
     8161 attcgaaaac tgaacgtaat tgattggctg ttgaatgaat aaaaactgcc tctaacatgg
     8221 gtagtcgttc ccgtaaaaca gggtaaatgt aagactgtct tttaaaaaaa tatactaaaa
     8281 gaagacgtca ttagaatcga ttaaagcgat gctaattttt cagatctgat acagaattct
     8341 agaggcaaag tggcttaaat ctgagttttt ttaaggtcat aactcactga ggacgaatga
     8401 gatttatacc cataacttgg ataagttgag aaaaacataa ttatattcca ttgattattt
     8461 tggatattgg gaagaaaagg actttttttg tgtctaaagt atatgccaaa tggagtatat
     8521 atatagatgt aatagccaat ttaatttatt gactctcatg gaaaaaatag aagttgcatt
     8581 taaggttact cttgatgacg tcaaggaatt tcaattttac cattataaaa aaagtattag
     8641 ttataagttc ttcatagtat gttgcatatt ttttgtccct ttaacattgt taattttctt
     8701 tgctgatcct aaacagaatt ggttacttgg ttgtttgggg ttcatatacc tatttttact
     8761 aattttttgg attccaatgc gtattaaacg caggagcaga ctcaatttca ccacaacaaa
     8821 ggcctatgag gacgaaatta attggacgat tgatgaaata ggatttgtag tcaaagggac
     8881 aaactttgaa ggcaaaaacc aatggactag tctgtacaag gttaaggaac ttaaaaatat
     8941 atttgtgttt tatagcaaca aatttgcagc aactatatta ccaaagcgag cattaacgga
     9001 gagaaacatg gctgaattaa agaagttttt caatttgaaa tctcctaaat aaatgtgcaa
     9061 gttgagttat acattgaaaa actggctata aaatcggatg agcattagca gggctgacaa
     9121 catttaagtt tacagatcag atggaagtat tgaggggaca gcaattgccc ggattttaac
     9181 aattattttt ggcacggttg actctaacat tgtaacaatt cgtgtaggtt cttacttaca
     9241 attggtaaat cttatctcat ttacacagta accgaccgag caactacaca tcatttttct
     9301 gtatataacc gggaagtaat tcatgtatcc ggttgatcgg gtgagatggg attttataga
     9361 gcgtttccaa gagccattca tagagattgg tgcttttatc cctgtttttc tggcaaaaca
     9421 cctttagaga gactggacat tgtatattga tccgccatca ttttctactt agtcatgatt
     9481 cttattttcc cgctaaggca tgaccattga tgacttgaaa aaagatgtag ttagacggtt
     9541 gcttacgaac aggcgtggta aggaatgtct taaaaaatgt aacagaccga gcaactatac
     9601 gtcatttttc tgtatataca aaagcccacc aaaaatcttc cgaattaaat cactaagccg
     9661 ggcggctttt gaatattcgg tgttcagcgg gaccgtatga tggactgtat ttctcttagg
     9721 tgtattcctg cattttttgt cggatggatg gctgacttaa ctccggaata tccaactgag
     9781 atttacacga aagtaacgta taggcagaga aagaatataa aaaggccgtt ggatagattg
     9841 gaaatttgag ggaaagctta cttttatacg gaaaatatac gccatatcgc atataaacag
     9901 atgttagtag tacttttaaa cgacaacaca gatcaaaatg aaaatatata gcaagtctca
     9961 gtttaaggat gaaacggccg accgaaacta ttttgaaaat tgggtgtccc ggttggaaaa
    10021 aaataacagc agacaatatg agcgatatga aattgaaacg tcacttggaa tgactcacat
    10081 ttggggtctc aacacaagag acgaacagtt agataccctt gttatttttc cgggagccag
    10141 aaccacctca ttaatttggg actttgacaa aggtcttgat aacttaaatc ataaaatgaa
    10201 aatttttatg gtagagacaa acggacttcc aaatttaagt gaaggtatga caccggacat
    10261 taaatcttta gactatggtc tttgggcgac agaagttttt gacaagctta atattgataa
    10321 agcatttgtt gccggagcct cttttggtgg gcttatttgt atgaaacttg gaatagtaaa
    10381 accggaaaga ataaaggctg catttttatt aaatcccggt tgcttacaac ctttttcttt
    10441 gacgctaaag aatctatact ataatctcct gcccataata aaaccgaatg aaagaaacgt
    10501 cttaaaattt cttgataccg cagttttttc taagccaaat cataagttat ccggattttc
    10561 ggaaaaaatg cttgttgact atgaggtttt tgcaataagc cgatatagag acaagacaca
    10621 aaagccatat tatatgggca aacagcttga agatgtaaaa gtagaaacct atttgcttgt
    10681 tggggacaaa gaccttttat tcccttatca aaagtcagtt gataatgcaa aaaggcaaat
    10741 taagacattg aaagaaacaa aaatttataa caacgtagga cacggaattg agacatacga
    10801 taaagcgtta aactatatag gagagaaaat aaaaaactgc cactaacagc ggtagtcgtt
    10861 gcacaactcc tcttttcaga aaagcaagct taaaaaggac tccatcagaa gcgatttgag
    10921 ggcggatgtc agtttatagg acaaatacaa ataaaaaaga tcggataggc cggaatgggg
    10981 ctgttgagtt catatgtcat tgttttcaga aaagcgtata accttacttg tatagaaaat
    11041 ccgggttggg cctttatacg gcatcctgtt gaaattatca ggcggttcta tgttatccaa
    11101 aggggtgtta tagaataatt ttggttcaga tttcttcctg cctcacatcc tttgagatga
    11161 gaaacctgcc aattgtttca aaatataaat tactattttg tatatttgag ttgtgcaaca
    11221 ggtatatcgg ttttgcgttt ggcggggtga cgtgaaaacc aggatctttg tactccgaaa
    11281 cccgcccgaa aggcaagttg aaaacggtta tgaataaggt taaacaacag aacagcagac
    11341 accaacaggc aggagaacca ccacctttaa tatgtgacaa ttcttgacga ttgcacttgg
    11401 taaaataatt ataaaagtgg agaccagaaa ccactgacaa aaacggggcg caaccgaaaa
    11461 gccaaacgag tagggaaaaa acaaagcgaa aaacatgaat tactatttaa aagttttaca
    11521 gaactatgcg acattcagcg gacgagcaag aagaagtgaa tattggtatt ttttcttgat
    11581 caatgtaatc atttcattta ttttgggttt tgtcggtggg gtgatgggaa catccatctt
    11641 gtccaacatt tatgtattag cagttttaat accggggatt gcagttggtg ttagaagaat
    11701 gcatgatgta gacaaaagcg gttggttttt attaattcca atttataatt tgattttagc
    11761 atgcacagaa ggaacaaaag gcgaaaacga atatggtgct gacccaaaag aagaagcata
    11821 aaatcaatta aacataggaa aaaaccagct catactactg gtaatcattg cacaactcct
    11881 tttttcaaaa aacaagttaa aaaaagtacc ccattagaag cgatttgaga ggagttgtta
    11941 gtatagagga tataatacaa ttaaaaagac cggatagctc ttcatggagc ctttgaggtc
    12001 atataccaaa gatttcaaag gaatgcagtc tgatgcatac atccggcttt ttgaagaagt
    12061 tatcgtttgg cactacgccg tccaacgtgg tgctaaataa taattattgc tccggccata
    12121 ccgggcagtt gggcgttaat gatacagtga atattccgga gcaaagtgcc ccatctgatg
    12181 cggatttttt tcatgataaa ttatggtgtc cgccagcgac atgtaataat attccttgtt
    12241 gaatgtcctg gcaatcaaat tttttgatgg acaaacagga tggaatttct tatttaaatt
    12301 gtaagcaacc gcctaagtat atcttatttt catctcatct ttagtcatac cagggaagaa
    12361 taataaaagc catgacgaaa cgagaaaaga cgacgtatcg atttgcaaag tctggcctta
    12421 atatagacgc ttttgcccta aagtagggac aatgtaatca acacaatgag gctatttaca
    12481 aagagccagg aagaaaagag catctaaaga aaaaaatgca taatcttctg aaactgaatt
    12541 tatggccata aagatcagtg cccgacacac tttttgtaca tgcaaaccat aaggaatcaa
    12601 cccctgtgaa tggctagtag ataccctcaa taaaatccca tctcacctga tcaaccgaat
    12661 acatgagctg ttcacgggtt ataaacagaa aaataatgtg ttgttgctcg gtcggttact
    12721 aacattaagc cgaatataca aaagcccacc aaaaatcttc caacttaaga cacgaagcca
    12781 gcccggcttt tgaatatttg ccattcagcc agcccggatg atggactgaa ttcctcttag
    12841 gtgtattcct gtcttttttg tcggatggat ggctggcttt tgctggtcaa agcaggaaga
    12901 aagaacttgt catccgaaat gaaaggcacg gcagcccact gcgcatggag ggctctttgt
    12961 tccggaaact cactccccgg ggtgtctcac tttacaccga aatacccaac tgaaatttac
    13021 acgaatataa cagagaagct gagaaaggat ataaaaagcc tgttggatag attggaaatt
    13081 tgagtgaaag cttactttta tacggaaaaa tatgcgccat atggcgtata gacagatgtt
    13141 accggtcagt gtaaaatgac agccatcgca cattccagca catttggttt tgtccaacac
    13201 tcaaagccga cccttcgcta aaccaaaaga gtttgaattt ttaccaccgc acggacgaca
    13261 aaactcagaa taaattgtat ttttgacgaa ttagtccaaa caataaagac aagatgaatt
    13321 cttttggaga atatttacga agcagacgag aacaactggg acttccactc agaaaagttg
    13381 ctgccgaact cgacattgac acgtctattc tgagcaagat tgagagaagc gaaagagttg
    13441 caacaaaaga aatgctttca acactggcaa agacgctgga cgttcaagag aaagaaatgg
    13501 aaattgaatt cattaaaaca tttatactat cgtacttagg agaattaaaa tttttgaaaa
    13561 gcggcttgga agaaacgctg aacactatta aaagccgtaa ttaaatatga gtttagataa
    13621 taagatagac caaacagacc taactttctt caccaatgaa gaagggcata cattattaag
    13681 tagatttaaa tctaccctga aagacaccca attatttgat gtgttggtgg gttattttag
    13741 agcaagcgga ttccatcaat tgtatgacgc attagaacct attgagaaaa tcagaattct
    13801 tgtcggctta aatgtagata gagattcgta tgaaataatg caataccatg aacaaagttc
    13861 tacgattgac tttgaatcgc atcagcggac aaaaaaacgg taccaacaga acctaaaaga
    13921 agaaattgaa aacagccacg aaaacgacaa cctacttgaa ataggaattc gcaaatttat
    13981 tgagtttctg aataccgact gccaagaccc tgcaatggat aaggcataca agggcaatgg
    14041 taagaaactt gaaatacgag catacccaac caaaaacatt cacgctaaag tttatatcgg
    14101 aaagttcaag cctgaagatc gagactatgg ttttgtaatt acaggttcaa gtaatttttc
    14161 tgaatcaggt tttgttgcaa accgagaatt caatgttgag ctaagaaata aaagcgatgt
    14221 actcttcgct gaaaatcagt ttaatcagct ttggagagaa tcagtagata tttcagaaga
    14281 ttttgttgac acgattcaga acaaaacttg gctcaatgac caaatcaaac cttatgaact
    14341 ctatctgaaa ctgatttacg agtatctgga agaagacatc aatttagctg acgagtttga
    14401 accattcctc ccagaaggtt ttatgaaatt gaaataccaa aatcaagcat ctatccaagc
    14461 aaaaaagatt ttggaaagat acaatggagt tttcttagca gatgtagttg gtttgggtaa
    14521 aacctttatc actgccttgt tgcttcaaca attacaaggc agaacgctag tgatttgtcc
    14581 gcctgtactt atagaatact ggaaagattc tctttttgat tttggtatca gaagttttaa
    14641 ggtagaatct ttgggtaaat tggaacacat catcaagaat ggtctggaac gctacgacta
    14701 cattgtagta gatgaagcac accgattccg gaatgaaaac acacaatctt atgccaactt
    14761 gcttgacata tgccgtggca agaaagtaat tttggtaact gcaacacctc taaacaatac
    14821 ggttgacgac attttcgctc aattgaaatt atttcaagca cctaaaaacg caactattcc
    14881 aggtattcca aatcttgaaa aatactttgc aagtttcagg acacgtttag ctaagcttga
    14941 aaagactgac cctgaataca aaaaacttat caagaacata tcagacgata tcagaaacag
    15001 catcttgcgg tatgtaatgg ttcgtagaac tcgaacagat gtaatgacct attttaaaca
    15061 agatatgaaa cttcaagggt tgacttttcc taatttagac aatccacaaa aaatcgttta
    15121 caaatacgaa ggtaatttag aaatagtttt caaccatact atcaaaaaac ttcaggagtt
    15181 tacttatgcg cgatatacac ctttactatt ctacgtcggc aataaagcat tgagcgaatt
    15241 tgaaatgcag cagcaacgaa acgttggagg tttcatgaaa ggtattttgg taaagcggtt
    15301 agaaagcagt ttttacgcat tccgccaaag tgttgaccgt ttcattacat cttatgagaa
    15361 gttcatagct atgtatcaaa acggtacggt ttatatcagc aagaaagtgg atgtatatga
    15421 attgattgaa agtgacaatt ttgaacgtct tgaaaaattt gtcgaagaag agaatgtaca
    15481 gaaattcgat tcaaaagact ttaaaaagga gtttattgat aaattggagt ttgacctttc
    15541 catcttgaag gaagtgaaaa atttatggca aaatgtaaac tccgatccca aattagaaat
    15601 gtttattcat gaacttaaaa ctaaaacttc actgaaaaac aacaaactgg taattttcac
    15661 cgaatccaaa gaaacaggag attacattta cgatgcactt caagacgagt tcagtggcaa
    15721 ggttatgttt tattccagca caggtggaag acacacagat aaaaatctga cttcaaatca
    15781 caccatttcc agagatatta ttacagcgaa cttcgacccg aaatataaag aaaaaagaga
    15841 cgatcttaaa atactgataa ccactgacgt tttggctgaa ggtatcaatt tgcatcgttc
    15901 caatgtgctt attaattatg atttgccatg gaatcccacc cgagttttac aacgtgcagg
    15961 gcgagtcaat cgcctgggaa gcaacttctc cactgtatat atcttcaatt tcttccccac
    16021 tacacaatca gacgagcatc ttggattaga aattaacatc actaacaaaa tccaaatgtt
    16081 tcacgacatt ctgggtgaag atgctaaata cctgagtgat ggagaagaat tcggcagtca
    16141 ggaactattc aatactttga atagtaaaac agcctataca ggtgaagatg tcgaaggcga
    16201 ttctgaattg aagtatctgg aaatgatgag aaaaatcaga gacgaacaac ccgacttgtt
    16261 tgaaaaaata agaaacctcc caaagaaagc ccgctccgga tttaaaaaag aaaaattgga
    16321 tgatgatgct ttagttactt tcttccgtat aggaatgctt aaaaagtttt acatcaacct
    16381 aggtgataaa tcaaatgaaa ttaccttttt tgatgcggtt agagaaatgg aatgcagccc
    16441 ggataccaaa cgtgaaaaca taccaaatac ctattatcat atgctgcaga ccaataaagc
    16501 ccgctttgaa ttagatacta ctgatggtga tgaaccaacc aagggaagtg gcggacgctc
    16561 taacctaaaa tatatcgaaa ccagattgaa agaaaagtcc ttcaaaaact tcaaaggatt
    16621 taccgaatcc aatgatgagt ttttaaacgg tgttcgtggt atgctggcaa atgggaccat
    16681 tgcaaagaaa acaacccagg taataaaagc agatttggaa aaaaccacag accctttaca
    16741 gatcttgcac atcctggaga agcatatccg ctttgttgca attgaaagag aacagatttc
    16801 caagaaattt cagaaccgtg aagttatcct ttcaggatac ttagtgaaat agaacgctga
    16861 tgacgcagat aaagcagatt aacgctgaat atggaatacg atacttataa acatactgac
    16921 atcacagaga aaataatcaa agcattttac aaggtatata atactttggg atatgggttt
    16981 cttgaaaaag tgtatgagaa tgcgttattt attgaactgc aatctatggg catatttgtt
    17041 gaaaaacaac gaagaataaa agtattttat ttagaacatg aagttggaga ttatttcgct
    17101 gacttgattg tggataataa agtgattgtc gagcttaaag cttctgaagc cttatgtgaa
    17161 gaacacgagt atcaacttat caattacctt aaagcgactg aaattgaagt agggctgttg
    17221 ctcaacttcg gtaaaagacc agagttcaaa cgaaaaatat tttcaaatga atgatagaac
    17281 actgataacg cagatacaac tgattcccgc agataagatc agcgtcaa
//
LOCUS       DAOQNP010000069        17078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1183_length_17078_cov_8.249721, whole genome shotgun sequence.
ACCESSION   DAOQNP010000069 DAOQNP010000000
VERSION     DAOQNP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17078
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1183_length_17078_cov_8.249721"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..268)
                     /locus_tag="PKY09_09620"
     CDS             complement(32..268)
                     /locus_tag="PKY09_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13157.1"
                     /translation="MTDNTDTKIGQMESLLSKWEKKKQNGDLKFNDYITIQQEIIKID
                     SNASFLNQESNFTTNQIKKSKELSLRLEKLITIK"
     gene            complement(346..948)
                     /locus_tag="PKY09_09625"
     CDS             complement(346..948)
                     /locus_tag="PKY09_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13158.1"
                     /translation="MNKSKFPFSAAITTFLVASLIFTGCGQGSIKIESVDDEVKASST
                     AEQLFLNVKSKISSEEKNKIAELTGLHYSPATKKFDSEGEEVEVQIVPVDINHDQSED
                     IFVITYSTYLYGNTGQGFSLLMKNESEQYSNVLSLPGIPQIIDDKTSSMPPIQIGGPG
                     FSFPVYTWKNHRYQLNKNYKINGSIQELDVLGNLYSSGFK"
     gene            complement(958..2022)
                     /locus_tag="PKY09_09630"
     CDS             complement(958..2022)
                     /locus_tag="PKY09_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13159.1"
                     /translation="MKKLTSQLLTVVMLIAGIWQCKAQETLSNQSIVSLVNAKMNNNI
                     IINKIKSSGNTFDLSTDGIIDLKATKVPENVINEMMLACRNMPVITNDDVIKMNGGKV
                     STDLILKKINLSRNNFNTSTDALIALKNAGVSDKIVKVMMNPPSNTASNVTTANIKEH
                     PQDLPPPTKLSIPGIYYEQFTPKQDYIMIEPTTTNQTKKGGFGESLGNHYSGGISGIS
                     TKVGLAGEHSSTTITDQRPVFYFYLSIDGKDINEVKESTYEGVASPKEFVLVRATTSK
                     SGRQIQIARRSSFSQEEGFSKGVVEYKSVKVSERLYKVYFENDVPAGEYAFYYNKSSD
                     QKKSLKLYDFSLRNNITVTK"
     gene            2167..4245
                     /locus_tag="PKY09_09635"
     CDS             2167..4245
                     /locus_tag="PKY09_09635"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13160.1"
                     /translation="MRGLKFIAFFFLLNLYNANSEVLDSLLLKADGYRISDTIRAGVL
                     NSLAVELSSSDYEKGLKYADSAIVLSTAIKADKKLATAYFAKARNLSSGGDDSLAIIN
                     YQKAYLLYLKTGNFTACATSLQNMGISYYNLSDYSLAAERHSAALDIFKKVRNDRGTA
                     AALNSLGIIAMEVADYTKAMELYFESLKLAKKIEDSTLLANSYSNIGIVYKNLGDLNK
                     ALVYQNIALQLNLKRQLAYETAKCYNNIAIVYSVMEQSDKAIESYNKALAINEKLKYS
                     FGIASNYLNLGIELLATNKILEAYGSMKKAESLFKKIGNRNSEAVAIKVQSSLLMNAP
                     DSILRFLKIPVSQRNTLCLSMHQKALEVFKETEDISNVADVENEMSTLYERMGDLKNS
                     LHYFKEYSLHKDSMAVEDKKSEIERSELKFQFEAKEQQLKLENAQRSTALKEAEMKRK
                     YYMAGSVCLLLLSGGAFVAYKQRKDVLVKKKEVELNAAVKESEFKALRLQMNPHFMFN
                     SLNSIADFIDKNKSQTASDFAVKFSKLMRMILEYSEQQEITLKEEIDLLELYLKLESI
                     RLSPKLNYHIDIDKEADADNILILPMLIQPFVENSIIHGITPLNNSGNIKISFLIQQN
                     ELEIVIDDDGVGMHAAKTTNERKSMAMKITSERIALYNEKYHANASLQLIALNPGTRV
                     QLRMPLKYRY"
     gene            4261..5004
                     /locus_tag="PKY09_09640"
     CDS             4261..5004
                     /locus_tag="PKY09_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB13161.1"
                     /translation="MLKVVIVDDEQHCIDRLTMLLEKAKDRNIKLSGTATSVDEGLKC
                     IEKYNPDLVFLDVEIGGKTGFDLLRSITSPTFKVIFTTAHDRYAIQAIKFCALDYLLK
                     PIDEDDFNQAISKVVAIDNVPDISMQITELLKGIEAKPKKIGLPTINGIVFVAVSDII
                     RCQADGNYTEFYLNNKQKLVVSKTLKEFEGFLTPYNFFRVHNSNLVNLEFVKSYNKGK
                     GGFLILEDNSTVEVSSRRKDELLKKLSLI"
     gene            complement(5028..6182)
                     /locus_tag="PKY09_09645"
     CDS             complement(5028..6182)
                     /locus_tag="PKY09_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB13162.1"
                     /translation="MDKIRILVVEDESIVAKDIQQTLIRLGYDVPATASSAQNAYARL
                     EELEPDLVFLDIKLKGDQDGIHIAEHIKQKYDIPVIFLTSFVDKNTLDRAKITEPYGY
                     LVKPFNESDLQTTVEMALYKFQKDLQVRENERRFANALANVEEAILVADTEFRITFLN
                     PKAENITGFGNESAQGMSLFKLIKIEGDEYEIVNADNLGKLMKQDEILSIKQSTITIV
                     RDYSNIKADFTCSPIRDEKNQLLGYAVVIGSSKSEKSVVQPVTKPTEEKPVEAPSNPL
                     ENQVIQNSFFVKRGAMLVKVFLENIYWLQAMDNYVIIQTKEDQFVIHATMKDIETKLP
                     PEKFMRVHRSYIIALDKISHMDEGAVVINDKTIPIGKSYKEVFMNRINFL"
     gene            complement(6194..9517)
                     /locus_tag="PKY09_09650"
     CDS             complement(6194..9517)
                     /locus_tag="PKY09_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB13163.1"
                     /translation="MLFSSMVHGQHYNFTTYTIVNGMPSNQVNAVHQDKTGKLWIGTM
                     NGACWFDGTNFYRFEQDNPASFNPVKAFYEDSKGNLWMAITRGGIGVFNGIRKKFYTI
                     DNGLLSNNTTAITQDKTGRYWIGTSEGLNSFDGSRFLSYTILKGLVSNEVTCLLADSK
                     GNIWIGTTNGLSKYDGKQFTNFTINEGLTGNIIYQISELDDGSIWAGTNDGISVFQNG
                     KFSSFDISKGVSVKKVTAILEDYKKNKWFSTYGNGMCLIDHGRFFTLTKDEGLTDNNI
                     LCMKEDNEGNIWIGTPRGLCRYSGGRFITYTTENGFNDNRLLTAYTDQLNRIWFGIVN
                     GGLNYIHGNTVYTPPFESTLTHATIWSIAQDAEQNFWFGTSNGPALVSADLSKISFPF
                     DLLNNKIIYAVMTHRNGSIWFGTDRGIYIYKNNSLRVLNKLNGLQNDNIRYLFQDRQG
                     LVWVGTLQGIYFMHNETAVSLNDKLNLNKTPITSITQDSEENIIFSTFDFGVYIFSKK
                     KGTTALRHLDVGNGLFNNRILTVASDNNYLWMGTPLGIDRIDWNTYLKRDFTNIIHFD
                     KSNGFYGVETNAATYDKDGFLWFATVNGAVRYNPNSGYSKSTAPVVLLQSIKAYMREV
                     NWSEQGFAIDSISGLPVNPVFPYGYNNLNFHFTGIYFASPDALQYRWKLDGFDDYWVP
                     ATNLNIANYSNLPPGNYTFQVQATANGHDWSPSLTYDFKVKSPVWRTNFFYFIYFVAL
                     IILILVILKLRTRSLRNAQLLLRQKINERTHQLRENNMQLAKLSIVASETDNAVMIFD
                     HNQNLEFVNEGYTRMHGYTMDEIIRKQGSKLTQLSYNDNIKTIIKDCLEEHKSVIYET
                     KITHRNGQQLWTSSTLTPVFNDNDELSNIVVIDTDITLHKKMEQQIRESLDEKNILLK
                     EIHHRVKNNLQIIVSLFNLQSAYLQDKASHKILKEGQDRIRSMALIHDRFYQSDGTSR
                     IDFDDYIKRLCETIMQSQNANPQKIRLQIEAEKISLDIDTAVPCGLIINELVSNAIKH
                     AFGDEEGVIRVTFKKEDDYYLLSVADNGKGLPEGKDFDTADTLGIQLVNVLSDQIDGK
                     VTVENRNGLKVIIKFKPSY"
     gene            complement(9636..10631)
                     /locus_tag="PKY09_09655"
     CDS             complement(9636..10631)
                     /locus_tag="PKY09_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNB13164.1"
                     /translation="MNEFVRAAFSKEVVSEAARLYNIKFSDLTLLRDNVNIIYRFREG
                     DKEFILRLTHSSHQCFDTVNSEIYWINFLHENKIPVSNPLPSANNNLCEKVTSGDSYF
                     SVVNFTAAPGVELGEKNWNSNTIGQMGRITGMIHKCSKTYSPPETIKKRTIWPEKSNQ
                     MAETYLPESDLKVREYWKQLLSVVKQLPSTSDSFGVVHNDIHRGNFMEDKGRLTLFDC
                     EDACYTWFVNDIACPLFFAMNYNGVNDFNRRGWARYFMDSFMKGYQKHNRFNPEQLKL
                     MPELLHLRGIFVYAYLFKAWDVTKLNHAQLNYFNRMKHIAENGFDWLKPEDYLLN"
     gene            complement(10635..11819)
                     /locus_tag="PKY09_09660"
     CDS             complement(10635..11819)
                     /locus_tag="PKY09_09660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB13165.1"
                     /translation="MAVKTLQLFESYLPNAQNWFFRMLNNLPDTEVNVAAYRYYNEQF
                     VTQKINLLPMPEYVSPELLEGRDQAKNIFSKIINRISILRKNKYYFDYIGKWARENGI
                     EIIHTHFANMGWQYLELKKITGLPYFVSFYGFDYESLPFRFQEWKKRYQSLYEQADGF
                     VCEGAFGASILINQGCDPAKVHIVHLGVETSKIPAVARTKNEGELHLLQLSNYSQKKG
                     HIYSLQAFIQALNDCPNMTLTFVGNELENIKGNLEALVKQHNIEHKVRFIGFIEFTKL
                     YEFFGKYHVFIHPSCYSDTRDCEGGAPIVLLDAQCTGMPVISTTHCDIPEEVIHGKTG
                     LLTPEKDADALSGSIRTFYNMNNNTYQQFATAARQHVIENFDIKPCALQLQQLYNTIV
                     NH"
     gene            11963..13708
                     /locus_tag="PKY09_09665"
     CDS             11963..13708
                     /locus_tag="PKY09_09665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNB13166.1"
                     /translation="MSNFVVSARKYRPSSFDTVVGQGHITITLKNAIKSNHLAQAFLF
                     CGPRGVGKTTCARILAKTINCSNITADTEACNNCESCVSFNNGQSLNIFELDAASNNS
                     VEDIRSLVDQVRFAPASGHYKIYIIDEVHMLSSGAFNAFLKTLEEPPPYAIFILATTE
                     KHKIIPTILSRCQIFDFNRIKVEDISKHLQQIAKKENIEADPEALHIIAQKAEGALRD
                     ALSIFDQMVTFSGNKVTYKDVINNLNVLDYEYYFRITDQILPGNISSVLLTFNELLNE
                     GFDGHHFIAGLSGHFRDLMVAKDEITLQLLEVTEAVREKYKVQSKAVELSVLLKLIDI
                     CNSCDLSYKSVRNQRLHVEIALMKMCALSGKLTVNENAMAAKPVVVEKVAVEKTTVAT
                     PVAQMSKNVSAIASASQESAKPEIAKATTATKAEEPLATISLSGLNAKQAAPDKTEST
                     DVKSEKKNDRPVVIEELNRIWTLYAALQDNNGHTNLSKALLRTEPVVINGTEIHVTVD
                     NQVLRDNLLSLKSEFLEYFKRELQNDFLTLEVRVEVSEKVLDKPYTAREKFKKMAEKN
                     PELENFRKQFDLNFD"
     gene            13748..14632
                     /locus_tag="PKY09_09670"
     CDS             13748..14632
                     /locus_tag="PKY09_09670"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNB13167.1"
                     /translation="MIIDPTQVKTGEFHAMMLSAVAPRPIAFASTVDKNGKPNLSPFS
                     FYNAFGSKPPILVFSPARRVRDNTTKHTLQNVFETREVVIGAVNYNLVHQASLASCEY
                     PEDVNEFEKAGFTAVKAEKVKPFLIKESPVNFECKVLDVIETGKLGGAANLIICEILL
                     MHVHDDILNESGKIDPNKIDLVARMGGDYYCRASGSAVFAVPKPNHKLGLGFDKIPDA
                     IRLSEVLTGNDLAQLANEEAIPGAEEIRQFNISDGMKKFNEAIAADPSNAKLAKHLAA
                     RKLIHAGKIKEAWMLLLA"
     gene            14961..15905
                     /locus_tag="PKY09_09675"
     CDS             14961..15905
                     /locus_tag="PKY09_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13168.1"
                     /translation="MKKYLLLITIYCFLIQTSEAQINLVPNPSFEDTVACPTNVMMID
                     STVGWSSYRLSPDYFNSCNQNGHAGVPANDWGYQYAKTGDAYAGIVTYAKFATDFREM
                     IGIQLLQALTVGQKYYLSFYVNNSRDSSSHYNAASNKMGAKFLTTAYSQASPIPIDNF
                     AHIYSDSIITDSINWIKISGSFIANLAYQYLAIGNFFSDSTCTHILLDSLASMAYYYI
                     DDVKLSTDSDFVNSIHAIDNIDLVQIFPNPARDWIVVEGRNITSIEVFDLCGKLIIPK
                     ITVSSFKTQVDISTLSYGVYIVKTNTSIDTYRNKFIHF"
     gene            15918..16946
                     /locus_tag="PKY09_09680"
     CDS             15918..16946
                     /locus_tag="PKY09_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13169.1"
                     /translation="MKYTFTYLFALSILLCNLRFAYSQATSGNNANAPVGANDYIGWT
                     SGTNTPLVIKNEDAQPIHFYTNAGAGTFNNMRMIILGNGRVGIGINNPLWQLDVADEI
                     NISQPRNYYMIGGETVLHNAGTENIFTGVNCGKDILAGNVSGNMNTFNGFSAGEFASG
                     NDNVFIGHSTGRYSNGQSNIFIGTSAGINNLGDYNSIVGFTAGMYLTTGNENTFMGLR
                     AGECNTSGSNNTFMGALSGSSNAGGSNNTFIGAQAGSRAEQVNNAIAIGYKAEVRCND
                     CTVLGGKFVGINTTTPQTTLEVNGTITTKQLIIANENQKQDVTAMLNELKNEIAQLKE
                     QINQLTKN"
BASE COUNT         5406 a         2975 c         3193 g         5504 t
ORIGIN      
        1 gtttgatttt ttaattggtt ataattttga tttattttat tgtaattagt ttttctaaac
       61 gcagtgataa ttccttggat tttttaattt gatttgttgt aaagtttgat tcttggttta
      121 aaaaagaagc attagagtct atctttatta tttcctgttg tattgttatg taatcattaa
      181 atttcagatc cccgttttgc ttttttttct cccattttga cagtaggctt tccatttggc
      241 cgattttggt atctgtatta tcagtcatgc acgaaacagt gaatagtata agtaattttg
      301 gtattatgct aagccagttt ttcatcataa tgtagtagca taatattatt taaaaccgga
      361 gctgtataaa ttaccgagca catctaattc ctgtatcgag ccgtttattt tgtaattttt
      421 atttagttga taacggtgat ttttccaggt gtaaacaggg aagctaaagc cgggtccacc
      481 aatttgtatg ggcggcatag acgaagtttt gtcgtcaata atttgcggta tgccgggtaa
      541 tgacaataca ttactgtatt gttcactttc gtttttcatc agcagactaa aaccctgacc
      601 ggtattacca tacaaatatg tgctgtaagt aatgacaaag atgtcttcac tttgatcatg
      661 attgatatca accggtacta tttgcacctc aacttcctct ccttctgaat caaacttctt
      721 tgtagcaggg ctatagtgaa ggcctgtcag ctcggcaatt ttgttctttt cttctgatga
      781 tatttttgat tttacgttta ggaataactg ttctgctgta ctggaagctt ttacttcgtc
      841 atctacagac tcaattttaa tgctcccttg cccacaacca gtgaatatca gacttgctac
      901 aagaaaggtt gttattgcgg cagagaaagg gaatttcgat ttattcatag caggtattta
      961 tttggtaacg gtgatattat tgcggagtga aaaatcataa agttttaatg actttttctg
     1021 atcgctgctt ttgttgtaat aaaaggcata ctctcctgca ggcacatcat tttcaaaata
     1081 aactttataa agtcgttcgg aaacttttac acttttatat tcaacaacac ctttagagaa
     1141 accttcctcc tgagagaatg atgacctgcg tgcaatttgt atctgacggc cacttttgga
     1201 tgtagtggct cttaccaaaa caaattcttt cgggcttgca acaccttcat aggtgctttc
     1261 ttttacttca ttaatatctt ttccatctat agaaagatag aagtagaaaa caggtctttg
     1321 gtcggtaatg gttgtgctgg aatgttctcc agccaaaccc actttagtac tgatgccgga
     1381 aattcctccg gaatagtgat tgcctaagga ttctccaaaa ccacccttct tggtctgatt
     1441 tgtggtcgtg ggttcaatca taatatagtc ttgtttaggt gtaaactgtt cgtaatatat
     1501 tccgggtatg gacaactttg taggtggagg taaatcttga ggatgttctt taatatttgc
     1561 agtagttaca ttagaggctg tatttgatgg cggattcatc ataaccttaa caattttatc
     1621 agaaacacct gcatttttca gtgcaatcag tgcatcagta ctggtgttaa aattatttct
     1681 tgaaagattg atttttttca gaatgagatc ggtgctgact tttccgccat tcattttaat
     1741 aacatcatca tttgtaataa caggcatgtt acggcaagcc agcatcattt cgtttattac
     1801 attttcgggc acctttgtgg cttttaaatc aataatgcca tctgttgaaa ggtcaaaagt
     1861 gttacctgaa ctttttattt tgttgatgat aatgttgttg ttcattttgg cattaaccaa
     1921 cgaaacaata ctttggttgc tgagtgtttc ctgagctttg cattgccata ttcctgcaat
     1981 aagcatgact acggttaata gttgtgaagt taactttttc atttgatttt tgattattta
     2041 ggtcaaatgt aaagtgaggt aatatggcgt acaatctact tctcttagtt gcattgtttc
     2101 aatgagccaa tgtaagtttt ggccgattat ctgcatttct tatatttgaa gcccaacctg
     2161 actaaaatga gaggtctgaa atttattgct ttctttttcc ttttgaatct ttataatgcc
     2221 aattctgagg ttttggattc attgctattg aaagctgacg gctatcggat tagtgatacc
     2281 attagagcgg gagtgctgaa tagtcttgct gttgagctct cgtcatcaga ttatgaaaaa
     2341 ggattgaagt atgcagatag tgctattgtg ctgagtacag caataaaagc agataagaag
     2401 cttgctacag cctattttgc caaagcacga aacctgtcaa gcggaggaga tgattctttg
     2461 gcaatcatta attatcaaaa agcatattta ttatatctga aaacaggaaa ttttacggcc
     2521 tgtgctactt cacttcagaa tatgggaata tcttactata atttatcaga ctactctttg
     2581 gcagcagaga gacacagtgc agcattggat atttttaaaa aggtcagaaa tgacagaggt
     2641 acagcagctg cattaaatag cttgggtatc atagctatgg aagtggcaga ctacaccaaa
     2701 gccatggaac tttattttga aagtctgaaa ctggctaaaa aaattgaaga ctccacctta
     2761 ctagccaatt catattccaa tataggtatt gtgtataaaa atcttggtga tttgaacaaa
     2821 gcacttgttt atcaaaacat agcattacag ctcaatctga aaaggcaatt agcctatgag
     2881 actgcaaaat gctataacaa cattgcaatt gtttatagtg tgatggaaca gtccgataag
     2941 gctattgaga gttataataa ggcattggcg attaatgaaa aactcaaata tagttttggc
     3001 atagccagca attatctaaa tttaggaatc gaattgcttg ccacaaacaa aattttggag
     3061 gcttatggtt caatgaaaaa agcagaaagt ctgtttaaaa aaattggcaa tcgtaactca
     3121 gaagcagtag ccataaaagt ccaatcatca ctgttaatga atgcaccgga tagcatattg
     3181 agatttttaa agatacccgt ttcgcaaaga aatactttgt gtttgtcaat gcatcaaaag
     3241 gcacttgaag tctttaagga aaccgaagat atttccaatg ttgcagacgt agaaaatgaa
     3301 atgagtactc tttatgagag aatgggagat ttgaaaaatt cattacatta ttttaaagaa
     3361 tattctttgc acaaagacag catggctgtt gaagacaaaa agagtgaaat tgaacgaagc
     3421 gagttaaagt ttcaatttga ggcaaaggag cagcagttaa aactagagaa tgctcaaaga
     3481 agtacagctt tgaaagaagc cgaaatgaaa cgaaaatact atatggcggg tagtgtgtgc
     3541 ttgctgctgc tttcgggagg agcttttgta gcctataagc agcgtaaaga tgtgttggtt
     3601 aagaagaaag aagttgagtt aaatgcagca gtaaaagaat cagagtttaa agcattgcgc
     3661 ttgcaaatga accctcactt tatgttcaat tctttaaatt cgattgcaga ttttattgat
     3721 aagaacaaat ctcaaacagc ttcagatttt gctgtaaaat tcagcaaatt gatgaggatg
     3781 attttagaat attcagagca gcaggagata accttgaaag aggagataga tttgctggaa
     3841 ctttatttaa agctggagtc aatacgacta agtcccaaat taaattatca tattgacata
     3901 gacaaagaag ccgatgcaga taacattttg atactgccca tgctcataca accgtttgtt
     3961 gagaacagca tcattcacgg cattacacca ttaaacaatt ccggaaatat taagattagc
     4021 tttttaatac aacaaaatga gttggagatt gtaattgatg atgatggagt gggaatgcat
     4081 gctgcaaaga ctacgaacga aagaaaatcg atggcgatga aaattaccag cgaaagaatt
     4141 gctttgtata atgaaaagta tcatgccaat gcatctcttc agttgatagc cctgaacccc
     4201 ggaaccagag ttcagttgag gatgccatta aaatacagat attaatttat ttataacgga
     4261 atgttgaaag tagtaattgt tgacgatgag cagcattgta tagacagact aacaatgttg
     4321 ttggaaaaag ctaaagatag aaatataaaa ctgtcaggaa cagctaccag tgttgatgag
     4381 gggttgaagt gtattgagaa atataatcct gatttggttt ttttagatgt tgagatagga
     4441 ggcaaaacag gttttgattt gttgcgttcc atcacttctc caacttttaa agttattttt
     4501 acaacagcgc atgatcgcta tgccattcag gcaattaaat tttgcgcact cgattactta
     4561 ttaaaaccaa ttgatgaaga tgattttaat caagccattt caaaagttgt tgcaatagat
     4621 aatgttcctg atatttcaat gcagataaca gaactgctta aaggaataga agcgaagccc
     4681 aagaaaattg gactgccaac aataaacggg attgtttttg tagctgtttc agatattatc
     4741 cgatgccagg cagatggcaa ttatacagag ttctatttaa acaacaagca gaaattggtt
     4801 gtttcaaaaa cactaaaaga gtttgagggc tttttgactc cgtataattt tttcagagtg
     4861 cacaactcca atttagtgaa tctggaattt gtgaaaagtt acaataaagg caaaggagga
     4921 tttcttatac tggaagataa cagtacagta gaagtttctt cgcgcagaaa agatgaattg
     4981 cttaaaaagt taagcctgat ataaatttat aaccactaaa aagttgttta cagaaaatta
     5041 atcctgttca taaacacttc tttatagctc ttgccaattg ggatggtttt gtcattgata
     5101 acaactgcac cttcatccat gtgacttatt ttatccaaag caataatgta ggagcgatgt
     5161 acacgcataa acttctctgg tggcaatttg gtttcaatat ctttcattgt tgcatgaatc
     5221 acaaactgat cttctttagt ctgaataatc acataattgt ccattgcttg cagccagtat
     5281 atgttttcga gaaatacttt tacgagcatt gcacctcttt ttacaaagaa ggaattttgg
     5341 attacctgat tttcgagagg attggaaggt gcttcaacag gcttttcttc tgtaggtttt
     5401 gttacaggtt gaacaacaga tttctctgac tttgaagatc caatgacaac ggcatagccg
     5461 agaagctggt ttttttcatc acgaataggg gagcatgtaa agtcagcttt tatattgctg
     5521 taatctctaa caatagtaat agtactttgc tttatactca atatctcgtc ctgcttcatg
     5581 agcttgccca aattgtcggc attgacaatt tcatattcat cgccttcaat tttaataagc
     5641 ttaaacaaac tcattccttg cgcactttca ttgccgaatc cggttatgtt ttcagctttt
     5701 ggatttaaaa atgttatccg gaattctgta tcagcaacta aaattgcttc ttctacattg
     5761 gctaaggcat tggcaaatct tctttcgttt tcacgaacct gcaagtcttt ctgaaattta
     5821 tacaatgcca tttcaacggt tgtctgtaag tccgattcgt tgaatggttt aaccaaatag
     5881 ccataaggct cagtaatctt tgctctgtca agtgtgtttt tgtcaacaaa cgaagtaaga
     5941 aaaattacag gaatatcata cttctgtttt atgtgttctg caatgtgaat accatcctga
     6001 tcgcctttta gttttatatc aagaaacacc aagtcgggct ccagttcttc aagtcttgca
     6061 taggcattct gagcagatga tgccgtggca ggaacatcat atcccagacg aattaatgtt
     6121 tgctgtatgt cttttgcaac gatactctcg tcttcaacaa ctaaaattct tattttgtcc
     6181 atatttgaaa aatttaatac gatggtttaa atttaataat tacttttaat ccgtttctgt
     6241 tttctaccgt cactttcccg tctatctgat cgctcagtac attcactaat tgaataccta
     6301 aggtgtcagc agtatcaaaa tctttccctt caggtaatcc ttttccattg tcagcaacac
     6361 ttaacagata atagtcatct tcttttttaa atgtcaccct aattactccc tcttcatctc
     6421 caaacgcatg tttaatagca ttgctaacaa gttcattaat tatcaatccg catggtactg
     6481 ctgtatcaat gtccaaagat attttttctg cttcaatctg caaacgtatt ttttgaggat
     6541 tggcattttg cgattgcata atggtttcac aaaggcgttt gatatagtca tcaaaatcaa
     6601 tgcgtgatgt tccgtcactc tgatagaaac ggtcgtgtat caaagccata gaacgtattc
     6661 tgtcttgtcc ttctttgagg attttgtgtg atgccttatc ctgcaaataa gcactttgta
     6721 aattgaacag gcttacaata atctgcaaat tgttttttac cctatgatgt atttctttta
     6781 acagaatatt tttttcgtct aatgattcac gaatttgctg ctccatcttt ttgtgtaagg
     6841 tgatgtctgt gtctatgacc acaatattgc tcaattcatc attgtcgtta aacacaggtg
     6901 tcagcgtact ggaagtccag agttgttgtc cattacgatg cgtaatcttg gtttcgtaaa
     6961 taacagattt atgttcttcc aggcagtctt taatgattgt ttttatatta tcattatatg
     7021 aaagttgtgt tagcttactg ccttgcttgc gtattatttc atccatagta tagccatgca
     7081 tacgggtata accttcattc acaaattcta gattctggtt gtgatcaaaa atcataacag
     7141 cattgtctgt ttcacttgca actattgaaa gttttgcaag ttgcatattg ttttctctaa
     7201 gctgatgggt acgctcattg attttttgcc tcaatagaag ttgtgcatta cgtaaactac
     7261 gagttcttaa cttcagaatt actaagatga gaattatcaa tgcaacaaag tagatgaaat
     7321 aaaaaaagtt agtacgccaa acaggtgatt taactttaaa atcataggtc aaagatggac
     7381 tccagtcatg gccgtttgct gttgcctgca cctgaaatgt gtaattgccc ggtggcaggt
     7441 tagaatagtt tgcaatattt aagttggtgg caggtaccca atagtcatca aagccgtcaa
     7501 gtttccatct gtattgtaat gcatccggtg atgcaaaata aattcctgtg aaatgaaaat
     7561 taagattgtt atagccataa gggaaaaccg gatttacagg taaaccggaa atgctgtcaa
     7621 tggcaaagcc ttgttctgac cagtttactt cgcgcatata agctttaatg gattgcagca
     7681 atactacggg agcagtactt ttactatacc ctgagtttgg attgtatcga acagcaccgt
     7741 taacagtagc aaaccaaaga aagccatctt tatcgtaggt ggctgcattg gtttcaacac
     7801 cataaaaccc attgctttta tcaaaatgga tgatgtttgt gaaatctctt ttcagataag
     7861 tgttccagtc aattctgtca atgcctaatg gtgtgcccat ccacagataa ttattatcgc
     7921 ttgcaacagt taaaattctg ttattgaaca aaccgttacc aacatcaaga tgtctgaggg
     7981 cagttgtacc cttctttttt gaaaaaatat aaacaccaaa atcaaaagtt gaaaagataa
     8041 tattctcttc tgaatcctgt gtgatagaag tgattggtgt tttattgaga ttcaatttgt
     8101 cgttgagtga aactgctgtt tcattatgca taaaatatat accttgcagt gtgccaaccc
     8161 aaactaaacc ttgtctgtcc tgaaataaat aacgaatgtt atcattttgc aaaccgttga
     8221 gtttatttaa aactctcaag ctgttgttct tatatatata aattccacga tcggtgccaa
     8281 accatatact tccgttgcga tgtgtcataa cagcataaat aattttgttg ttaagcaaat
     8341 caaatggaaa ggaaatttta gataagtcag ccgataccaa agcaggaccg ttagaagttc
     8401 caaaccaaaa attttgttct gcatcctgag caatgctcca gatggtagca tgagtaagag
     8461 tgctttcaaa aggaggtgta tataccgtat tgccatgaat gtaattcaat ccgccattta
     8521 caattccaaa ccaaattctg tttaattgat ctgtatatgc agtaagtagt ctgttgtcat
     8581 taaatccgtt ttctgttgta taggtgatga agcgtccacc tgaatagcgg catagtccac
     8641 gaggtgttcc aatccaaatg ttgccttcat tatcttcttt catgcacaaa atgttgttgt
     8701 ctgtaagacc ttcatctttt gtcagtgtaa aaaaacgacc atggtctata agacacattc
     8761 cattaccata ggtgctaaac catttattct ttttataatc ttcaaggatg gcagtaactt
     8821 ttttaacgga aacacctttg ctgatatcaa atgagctgaa ctttccattt tgaaaaacgc
     8881 tgataccatc attggttcct gcccaaatac taccatcatc caattcagaa atttgataaa
     8941 taatatttcc tgtaagtcct tcgttaatgg taaagtttgt aaattgttta ccatcgtact
     9001 tcgacaagcc gttggtagtt cctatccaaa tattaccttt actgtcagca agcaggcaag
     9061 taacttcatt actcaccaag cctttcaaaa tagtgtaact taaaaatcgg gatccgtcaa
     9121 aactgttcaa tccctctgac gtgccaatcc aataacgacc cgttttatct tgtgtaatag
     9181 ctgttgtgtt attgctgagt agtccattgt ctatggtata gaattttttt ctgataccat
     9241 taaagacacc aatgccgcca cgtgtaattg ccatccacag atttccttta ctgtcttcgt
     9301 aaaatgcttt tacaggattg aatgatgccg gattgtcttg ttcaaagcga taaaaatttg
     9361 tgccatcaaa ccaacaggca ccgttcatgg ttcctatcca tagcttacct gttttatcct
     9421 gatgaacggc attgacctga ttgctgggca ttccattgac aatggtgtaa gtggtgaagt
     9481 tataatgctg accatgcacc attgaactga aaagcatcaa cagtacaatt atgaataaag
     9541 gtatttgaat aatactttta tttttttttg tcagacctat tttaacactc acttgaattt
     9601 ataattttta gcctactgtt tggaataata tagatttaat tcaaaagata atcttcaggc
     9661 ttcagccaat caaaaccgtt ttctgctata tgtttcattc tgttaaagta atttaactgt
     9721 gcatgattga gttttgttac atcccatgct ttaaatagat aggcatagac aaaaattcct
     9781 cttaagtgca gtaattccgg catcaattta agttgttcgg ggttaaaacg attgtgtttt
     9841 tgatagcctt tcatgaaact atccataaaa taacgagccc agcctctgcg gttaaaatca
     9901 tttacaccat tgtagttcat tgcaaaaaac aagggacagg ctatatcgtt tacaaaccag
     9961 gtgtagcaag catcttcaca gtcaaatagt gtcagtcgcc ccttgtcttc cataaaattt
    10021 cctctgtgaa tatcgttatg caccacgcca aatgaatctg atgtggaagg caattgttta
    10081 actacagaaa gtaattgctt ccaatattcc cgtactttta aatctgattc aggcaaatag
    10141 gtttctgcca tctgatttga tttttcaggc cagatagttc ttttttttat tgtttcagga
    10201 ggcgaatatg tttttgaaca cttatgtatc atcccggtaa ttcttcccat ttgtccaatg
    10261 gtgttgctgt tccagttttt ttcacctaat tcaacacccg gtgctgcggt aaaattcact
    10321 acactgaaat aagaatcgcc tgaagttact ttttcgcaga gattattatt tgccgaaggc
    10381 aatgggtttg atacaggaat tttattttca tgtaaaaagt ttatccagta aatctctgag
    10441 tttacggtat caaaacactg atgtgaagaa tgtgtaagtc ggagaataaa ttctttatcg
    10501 ccttcacgga aacgataaat gatgtttaca ttatcacgaa gtaatgtgag gtcagaaaac
    10561 ttaatgttat aaagcctggc agcttcagaa acaacctctt ttgagaatgc tgcacgtaca
    10621 aattcgttca tttattaatg gttgacaatg gtgttgtaga gttgttgaag ttgtagtgcg
    10681 cagggtttaa tatcaaaatt ttcaattaca tgttgtcttg cagcggttgc aaattgctga
    10741 tatgtgttgt tgttcatatt gtaaaaagtg cggatagaac cggataaagc atcagcatct
    10801 ttttcaggag taagaagtcc tgttttgcca tgtataacct cttcgggaat gtcgcagtgt
    10861 gtggttgaaa taacaggcat gccggtacac tgtgcatcga gcaaaacaat aggtgcgcca
    10921 ccttcgcagt cacgcgtatc agaatagcaa cttggatgaa taaatacatg atactttccg
    10981 aagaattcgt aaagcttcgt aaattcaata aagccaataa agcgtacttt atgctcaatg
    11041 ttatgttgtt ttaccaacgc ttcaagattg cctttaatat tctccaattc attgccaaca
    11101 aaggtcagcg tcatattcgg gcagtcgttt aatgcctgaa taaaagcctg aagcgaataa
    11161 atatgtcctt ttttctgact gtagtttgac agttgcagca gatgcagttc accttcattc
    11221 ttggttcttg ctaccgcagg aattttagag gtctccacac caaggtgcac aatatgaact
    11281 tttgcagggt cgcagccctg attaattaag atagaagcac caaaagcacc ttcgcaaaca
    11341 aagccatcag cctgttcata caaagactga tagcgctttt tccattcctg aaaacggaac
    11401 ggtaagcttt cataatcaaa gccgtaaaaa gagacaaaat agggaagacc cgtaattttt
    11461 ttaagttcaa ggtattgcca tcccatattg gcaaagtgtg tgtgaatgat ttcaatgcca
    11521 ttttctctcg cccatttgcc gatatagtca aaatagtatt tgttttttcg gagaatggaa
    11581 atacgattga tgattttcga gaaaatattt ttggcttgat cgcgcccttc aagcaattcc
    11641 ggactaacat actcgggcat gggcaataag tttattttct gtgtaacgaa ttgctcattg
    11701 taatagcgat aggctgcaac attcacctct gtatcaggaa gattattcag catcctgaaa
    11761 aaccagttct gtgcattagg caaataactt tcaaaaagtt gcagtgtttt tactgccatg
    11821 aaatatcaag tttttatttt acgctaaata cgacatttcc atttgtaatt tacatggttt
    11881 tcataaatct ttattaaaaa cttactgttg aaaccggaat ggaaaagtaa atgattaaat
    11941 ttgctaagaa tttagaatca ttatgagcaa ttttgttgtt tcagcacgca aatacagacc
    12001 ttcatccttc gacactgtag tagggcaagg gcatattacc ataaccctaa agaatgcaat
    12061 aaaaagtaat catcttgctc aggcattttt gttttgtggg ccaagaggag taggtaaaac
    12121 aacttgtgcg agaatccttg caaagaccat taattgcagc aacatcactg ctgacacaga
    12181 agcatgtaat aattgtgaat catgtgtttc gtttaataac ggacagtcgc tcaacatttt
    12241 tgaattagat gcagccagta ataactcagt agaggatatc cgcagtttag tagatcaggt
    12301 acgttttgca cctgcaagcg ggcattataa aatttatatt attgatgagg ttcacatgct
    12361 ttcctctggt gcatttaatg ctttcctgaa aacattggaa gagccgccac cttatgctat
    12421 ttttatttta gcaacaacag agaaacataa aattattcca actattttat cacgttgtca
    12481 gatttttgat ttcaaccgta taaaggttga agatatttca aaacacctgc agcaaattgc
    12541 taagaaagaa aatattgaag ccgaccccga agctttgcac attattgcac agaaagccga
    12601 aggtgcattg cgtgatgcat tgtctatttt cgatcagatg gttacttttt cgggcaataa
    12661 ggtaacctat aaagatgtca tcaataacct taacgttctc gattatgaat attatttccg
    12721 cataaccgat cagattttac cgggaaatat ttcatctgta ttgttaactt ttaatgagtt
    12781 gttgaacgaa ggttttgacg gtcatcattt tattgccggg ctgtcagggc attttcgtga
    12841 tttgatggtt gccaaggatg aaattacatt gcagttgtta gaagtaacag aagcagttcg
    12901 cgaaaaatat aaagttcaaa gcaaggctgt tgagttatct gtcttactaa aactgataga
    12961 tatttgtaat tcatgtgatt tatcatacaa gagtgtccgc aatcagaggc tgcatgtaga
    13021 aattgcactg atgaaaatgt gtgccttgtc aggaaaactt acggtgaatg aaaatgcaat
    13081 ggcagcaaaa cctgtagtag tagaaaaagt tgctgttgag aaaactactg ttgcaacacc
    13141 tgtagcacag atgtcaaaaa atgtatctgc tatagcttcg gccagtcagg agagtgcaaa
    13201 accggaaata gcaaaggcaa ctacagcaac aaaagctgaa gagcctcttg ccacaatttc
    13261 tttatcagga cttaatgcta aacaagcagc acctgataaa acagaatcta cagatgtaaa
    13321 atctgaaaaa aaaaatgaca ggccagtagt aattgaagag cttaatagga tatggacttt
    13381 gtatgcagca ttgcaggaca acaatggcca taccaacctt tcaaaggcat tgcttagaac
    13441 agagcctgta gtgattaatg gtacagaaat acatgttact gttgacaacc aagtactccg
    13501 tgacaatttg ttgtcgttga aaagtgaatt tcttgaatat ttcaaaagag aattgcagaa
    13561 cgattttctg actttggaag ttcgtgttga agtatcagag aaagtgcttg ataaacccta
    13621 tactgcaaga gaaaaattta agaaaatggc agagaaaaat ccggaacttg aaaatttcag
    13681 aaagcaattt gaccttaatt ttgattagtc aacaataaat ttcagtgttc ttttatagag
    13741 gtaaataatg ataatagatc ccacgcaagt taaaacaggt gagtttcatg caatgatgct
    13801 tagtgctgtt gcaccacgcc ccattgcctt tgccagtacg gttgataaaa atggtaaacc
    13861 aaacctgagt ccttttagtt tttataatgc ctttggcagc aagccgccaa tccttgtttt
    13921 cagccctgcg cgcagagtgc gagacaatac gacaaaacac accttgcaga atgtttttga
    13981 aacacgtgaa gtagttattg gtgctgtaaa ttataattta gtacatcagg catcattagc
    14041 aagctgcgaa tatcctgaag atgtgaatga atttgaaaaa gcaggattca ctgctgttaa
    14101 agcagagaag gtaaaacctt ttctgataaa agaatcgcca gtaaattttg aatgtaaagt
    14161 tttagatgtt attgaaaccg gaaagttagg aggagcagct aacttaataa tttgcgaaat
    14221 attactgatg catgttcatg acgacatatt gaatgaaagc ggaaagattg acccgaataa
    14281 gatagacctg gtagcacgta tgggaggtga ttattattgc agggcatccg gtagcgcagt
    14341 atttgcagtc ccaaaaccaa atcataaatt gggtttaggt ttcgataaga tacctgatgc
    14401 aataagatta agcgaagtgc taacgggcaa cgacttggcg cagttagcaa atgaggaagc
    14461 aatacccgga gcagaagaga tacgtcagtt caatatatct gatggtatga aaaagttcaa
    14521 tgaggcaatt gctgctgacc cttcaaatgc aaagcttgcc aaacaccttg cagcacgcaa
    14581 attgatacat gccggaaaaa ttaaagaagc ctggatgttg ttattggcat gataaaacaa
    14641 gacaagtaag ttgttaagtc cggtattatt gtgtattaat gtaagatagt gttcaataaa
    14701 aaaagatccc aaaacatttg tttttaaaat ttaaacactt acttttacac tactaaataa
    14761 ccatgccatg ttagaaaata aactttgttt gcctttatcg gggagttggc tgcgtttttc
    14821 ttagcccgca gccacaacag aaatcttttt tacatttctt gcttttacaa attattaatt
    14881 aaataatcca caaatcttta attgggtaaa atgcaaggtg atgcacctgt tttggattta
    14941 aggatgaaaa gaaactcaaa atgaaaaaat atttactatt aattaccatt tactgctttt
    15001 taatacaaac atcggaagca caaattaatt tagtacccaa cccgagtttt gaggatactg
    15061 ttgcttgtcc aactaacgta atgatgattg atagtactgt aggatggagc agttaccgat
    15121 tatcccctga ttattttaat tcatgtaatc aaaacggaca tgcgggtgta ccagctaatg
    15181 attggggata tcaatatgct aaaacaggtg atgcttatgc aggaattgta acttatgcaa
    15241 aatttgcaac tgattttaga gaaatgatag gtattcagct attgcaagct cttacagttg
    15301 gacaaaaata ttatttatca ttttatgtta ataactctcg ggatagctca agtcattata
    15361 atgcagcctc taacaaaatg ggagctaaat ttttaaccac agcgtattcc caagcaagcc
    15421 ctattccgat agacaatttc gctcatattt attctgattc aataattact gattccataa
    15481 attggataaa aatttcaggc tcttttatag ctaatttagc ctaccagtat ttagcaatag
    15541 gtaatttttt ttccgattca acttgtacac acatcttatt agacagcctc gcttcaatgg
    15601 cttattatta cattgatgat gtcaagcttt ccaccgattc cgactttgtt aattcaattc
    15661 atgcaattga taatattgat ttagttcaaa tttttccgaa tcctgcaagg gattggatag
    15721 tagtggaagg aagaaatatt acatcaattg aggtatttga tttatgcgga aaattaataa
    15781 ttcctaaaat aacagtttct tctttcaaaa cacaagtaga tatttctact ttaagctatg
    15841 gagtttatat tgttaaaaca aatacctcaa ttgatacata cagaaacaaa tttattcact
    15901 tttaaaatca aaatattatg aaatatacat tcacatattt atttgcattg agcatattgc
    15961 tctgcaattt aagatttgct tatagtcagg caacatctgg aaataatgct aatgctcctg
    16021 ttggagcaaa tgattatatt ggctggacaa gcggcactaa cacacctctt gtaataaaaa
    16081 atgaagatgc acaacccatc catttttaca ccaatgcagg ggcgggtact tttaataata
    16141 tgcgaatgat catacttggc aacggcagag taggaatagg aatcaataat ccgttgtggc
    16201 aattagatgt agccgatgag attaacatat ctcagccacg caactattat atgataggag
    16261 gcgaaacagt gcttcacaat gccggaacag aaaatatttt taccggtgta aattgtggaa
    16321 aagatatttt ggcaggaaat gtttcgggaa acatgaatac tttcaatgga ttttctgccg
    16381 gtgagtttgc ttccggaaat gataatgttt ttattgggca tagcacagga cgatatagta
    16441 acggacaatc caatatattt attggtactt ctgccggcat taacaatctt ggtgattaca
    16501 attcaatagt tggttttaca gctggaatgt atttaaccac aggaaatgaa aataccttta
    16561 tgggattacg cgcaggcgaa tgtaatacat cgggcagcaa caataccttt atgggtgcac
    16621 ttagcggaag ttcaaatgca ggtggcagca ataatacttt tataggcgca caggcaggaa
    16681 gcagagcaga acaagtgaat aatgccatag ccattggcta caaagccgaa gtaagatgta
    16741 atgactgtac agtacttggt ggaaagtttg taggtataaa caccaccaca ccccaaacca
    16801 cccttgaagt aaacggcact ataaccacca aacaactcat catagccaac gaaaatcaaa
    16861 aacaagatgt aacagccatg ctcaacgagc tgaaaaatga aatagcccaa ctgaaagaac
    16921 aaattaatca attaacaaaa aattaaaggt gatgaaaaaa cttatactat taattttaac
    16981 tttcgcttgc acgttttgta atgcacaaat taatttagta cctaacccct cctttgaaga
    17041 taccatagag tgcccccatt tttccaatca agtggata
//
LOCUS       DAOQNP010000070        17062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1184_length_17062_cov_7.963544, whole genome shotgun sequence.
ACCESSION   DAOQNP010000070 DAOQNP010000000
VERSION     DAOQNP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17062
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1184_length_17062_cov_7.963544"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PKY09_09685"
     CDS             complement(<1..656)
                     /locus_tag="PKY09_09685"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNB13170.1"
                     /translation="MKKFLRITIIAFVVLLAAALVAPFIFKDKIIAAIKNAANENLTA
                     TFDFKDLDLSFIRSFPSVYLALNDVSIVGKDVFKGDTLISASSVQIKAEFWKLITSGN
                     TQIKYFGLIQPRIHLIALKDGKVNWDITKPEKETPAKAEESSPFHFSLKKYEITNGYL
                     SYNDEALAMSTVLNNLNHTGSGDFTQDNFLLKTLTTIDKLTYTYAGVSYLYQAKVNLK
                     A"
     gene            833..2746
                     /locus_tag="PKY09_09690"
     CDS             833..2746
                     /locus_tag="PKY09_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNB13171.1"
                     /translation="MNLFRKKTVNEIMKRAGEDASEHGSLAKHLGVRDLTALGIAAII
                     GAGIFSTIGNASAQGGPGVILLFLFTAIACGFTAFAYAEFASLVPVSGSAYTYSYVAF
                     GEFIAWIIGWALIMEYGIGNITVAISWSDYLTGLLDSMGMHLPLWMTMDYLTAAKGFS
                     KAGALMLSGRTLESLDAGVRDAYLAWQGAPTIGPLHIVADLPALLIILIITWIVYRGI
                     KESKNAGNAMVIVKLSVVLLVIAVGLFYINTDNWHPFLPNGIPGVLKGVSAVFFAYIG
                     FDAISTTAEECKNPQRDLPRGMMWAIIICTVLYIAIALVLTGMVNYSTLAVGDPLAYV
                     FSALNLKWMSAIIAVSAIVAMASVLLVFQLGQPRIWMSMSRDGLLPKKFATIHPKYKT
                     PSFASIVTGFVVAIPALFMNLTMVTDLCSIGTLFAFVLVCAGVLKMQITPNAPRGSFK
                     TPYVNAKFIFPVMLIVAMILSLTYNKEGVTNFLKNTPQPYATENLISSLGAERIKDLM
                     TTVSLKDADAFASSDNDLATYLSQLKEDTYLQKLTEYGLSHKEIYESGLELFKHKIPT
                     WIFIISCLYLCVLTWKYNFSLIPLLGLVCCLYMMSEMGISNWIGFSIWLAVGLVIYFG
                     YSYKNSKLNFLKK"
     gene            2752..3510
                     /locus_tag="PKY09_09695"
     CDS             2752..3510
                     /locus_tag="PKY09_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNB13172.1"
                     /translation="MPSIFITGATAGFGRAIALKFAQHHWSIIINGRRTDRLRTLEEE
                     IKNLGADVISLPFDVRNEQQVMEAIENLPNKWKHIDALVNNAGLAAGVNLIQDGKTDD
                     WNTMIDTNVKGLLYVTKNIIPLMIKQKSGHIINIGSIAGKDAYMKGNVYCATKYAVDA
                     LTKSMRIDLLEHGIKVSAVNPGAAETEFSLVRLKGDTEKANAVYKGFQPLVADDIAEI
                     VYFVATRPAHVCINDVVVTPTAQANTAHLLRKAD"
     gene            complement(3511..5217)
                     /gene="asnB"
                     /locus_tag="PKY09_09700"
     CDS             complement(3511..5217)
                     /gene="asnB"
                     /locus_tag="PKY09_09700"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNB13173.1"
                     /translation="MCGIAGIINFQHKPVDLSVLKQMSDTISYRGSDGEGVWMNEQKN
                     LGLAHRRLAIIDLSEEARQPMHYLGRYTIVYNGEIYNYLELKADLEKKGYQFVSHSDT
                     EVILAAFDNKREKCLEDFDGMFAFAIWDNKLQQLFLARDRFGEKPLRYYMDSERLVFA
                     SEIKALFKAKVPKKVSKEKMENFLENNVSIYNTETFFDGIKKLPHGHYAIVQNGKIDI
                     REYYRLQYRNIVAMNDHECAEEFRRLFFLSISRRLRSDVPVATSFSGGLDSSGIVCVM
                     HQLREKVNYKLFSARFDDDVKDEGKWMKYITDSFHFPQINKQIEPKEILEEIEKIVYH
                     NEIPIGSTSVAAQYLLYKEVKNHHIKVILDGQGADEMLAGYGHYRFHYLNDLLLRFRV
                     MEYMNERRAYNDRYQNSLPIGPWQLTKMFLTKTVRPFEDNTLGYQSFKAALFNDMVQN
                     LQQLLNFSDSNSMAHGVEARLPFLYHKLVDFVFSLPANQIYRNATTKYVYRNAMKGII
                     PDVILNRKDKLGFAPPQERWLPQFKLPEKSRLREMGLTYSDNHWRNYISEVFLKVADT
                     RY"
     gene            complement(5292..5759)
                     /locus_tag="PKY09_09705"
     CDS             complement(5292..5759)
                     /locus_tag="PKY09_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNB13174.1"
                     /translation="MHIKLLMIGKNKDSFAEAAIVEYANRVERFNGFEIKYLKEAKNN
                     KNLTAAMDVEADNIFKELSPNDYVILLDEKGKEYTSVQFAAKIEQLRLKNFRQLVYIA
                     GGAYGFSEKVYTRSNELFALSKMTFTHTHARLIFAEQIYRAFTILNKHPYHHI"
     gene            5965..6354
                     /locus_tag="PKY09_09710"
     CDS             5965..6354
                     /locus_tag="PKY09_09710"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNB13175.1"
                     /translation="MKKTIKLLLSVLLIATVVKAADVFDDIGAAIRSGDAKQIARFFN
                     NNVDLTIFNQEEVYSKAQAEMVLKDFFSKNAPKSFTIIHKGVSKEGARYAIGTFTTTQ
                     GQNIRTYFFVKESGGTASIQELRFEKE"
     gene            6491..7327
                     /gene="nadC"
                     /locus_tag="PKY09_09715"
     CDS             6491..7327
                     /gene="nadC"
                     /locus_tag="PKY09_09715"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNB13176.1"
                     /translation="MDLDQYILLSLQEDVGPGDFSSLSCIPAHASSKAKLIIKDKGIL
                     AGVELAERIFKTVDSSLQINIHIKDGQHVDKGEIGFTIEGSVLSILKSERLVLNCMQR
                     MSGIASTTHLYAEQVKHTKAKILDTRKTTPLNRYIEKWAVRIGGGHNHRFGLSDMIMI
                     KDNHVDYAGGIKQAIDAANVFRKTRNLNIAIEIETRNLNEVSQVLSTGGVERIMLDNF
                     QPTLLKEAVALIGGRFETEASGGIILQNIKEYAETGVDYISVGALTHSFISMDMSLKA
                     TR"
     gene            7324..8250
                     /locus_tag="PKY09_09720"
     CDS             7324..8250
                     /locus_tag="PKY09_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNB13177.1"
                     /translation="MKRRLLEYLSLIINYTRRITLPGFQKIPIYDVGKFFLRELFKGD
                     LQIRARAIAFSFFLAIFPAIIFLFTLIPYVPIDGFQERLLSILQSLLPYNTYDTAYKT
                     IEEIIVRQNGGLLSIGFILALFVSTNGVFALIEGFNKTIMHEKRKGWKQRLVALYLTI
                     LLATLLIIAILLIIITEVILHYFNNHILNLNQNSVYLLLAGKYLILILLCFTAISSLY
                     FFGPSGGSKKWHFFSPGSILATILIAVTSIAFNFFILNFGQYNKIYGSIGTLIVILIY
                     LNFNCIQLLIGFELNNAIEKAKFKPNTEEIKS"
     gene            complement(8247..10727)
                     /gene="lon"
                     /locus_tag="PKY09_09725"
     CDS             complement(8247..10727)
                     /gene="lon"
                     /locus_tag="PKY09_09725"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNB13178.1"
                     /translation="MFNPEDIKHTLIGGINDDEPELIPLLSSEDEEQLNKESIPDELS
                     ILPLRNTVLFPGVVIPITVGRDKSIKLIKDAYKSNKIIGVVSQKDTNIEDPGFNDLNK
                     IGTVASVIKMLRLPDGNTTIIIQGKRRFSLLEMTQSEPYLKAKVEPLNDIVAALKEKE
                     FKATVDSLKDISLQIIQHSPQIPSEAGFAVRNIESPAFLINFISSNMQMEVSKKQDLL
                     EMTDIKQRALKVLEYLTQELQMLELKSQIQNKVKVDLDKQQREYFLNQQLKTIQEELG
                     GSTPDQELQQLKTRAKSKKWNKHVAETFNKEIERLGRINPAAAEYSVTLNYLDLLLEL
                     PWGEFTEDNFNLKNAQKVLDKDHYGLEKVKTRILEYLAVLKLKKDMKSPIMCLYGPPG
                     VGKTSLGKSVAKALGRKYVRVSLGGLRDEAEIRGHRKTYIGAMPGRIIQNIKKAKSSN
                     PVFVLDEIDKLTNDFHGDPSSALLEVLDPEQNNAFYDNYIELDYDLSNVLFIATANSL
                     SSIQPALRDRMEIIEINGYTVEEKIQIAVKHLIPKQLDAHGLKKHHATFNNSVIEYII
                     EFYTSESGVRSLERNLASVIRSIAKRVALNEKYDKKVDKDAVEKALGAPIFLRDRFAD
                     NSVAGVVTGLAWTSVGGDILFIETSLSRGKGRLLLTGNLGEVMKESAQIALAYLKAHA
                     ADFGIDYHVFDRWDIHIHVPEGATPKDGPSAGITMLTSLASAFTQRKVKLKLAMTGEI
                     TLRGKVLPVGGIKEKILAAKRAGITDIILCEDNEKDIKDIKSEYLKGLRFHYVSKMGE
                     VIDLALLQDKVKKPLNFTISQPERKEVF"
     gene            complement(10797..11975)
                     /locus_tag="PKY09_09730"
     CDS             complement(10797..11975)
                     /locus_tag="PKY09_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HNB13179.1"
                     /translation="MNIILADSNRVDLLPFTFIRPVAEIRCGILTITEKWHHLSGTPC
                     SWLTEKYLSTKYSLTKEKVNLIANASVLIDKALFNEVKTLKTGQALVKGNTVIAVCTE
                     QQQFESLEDVSYSEAFETGLEVNVIKYPWDIFRFNDKAINSDFQLITEGRKSADYDSS
                     NTIINEQQVFIEGGAKVMCSIINASAGPVYIGRDSEIMEGCMIRGPFALGEHASLKMG
                     AKIYGATTIGPHCKVGGEVNNSVIFGYSNKAHDGFLGNAVIAEWCNLGADTNNSNLKN
                     NYGIVKAYNYHKKDYINTGLQFCGLMMGDHSKAAINTMFNTGTVTGICANIFGSGFPP
                     KFIPSFAWGGFDDESFFQLDKALELAAEVYKRRDLFFDKKEQDIINHVNMLELILPKN
                     "
     gene            complement(12135..13076)
                     /gene="mdh"
                     /locus_tag="PKY09_09735"
     CDS             complement(12135..13076)
                     /gene="mdh"
                     /locus_tag="PKY09_09735"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNB13180.1"
                     /translation="MKVTVVGAGNVGTTCANVIAHRELVNEVVLLDIKEGTAEGKSLD
                     MWQTAPINLYDTRIKGSTNDYAATAGSDVVVITSGLPRKPGMSRDDLIATNAGIVRSV
                     TENIVKHSPNTIIIVVSNPLDVMTYCAYLTSGFDSKRVFGMAGILDTARYRAFLAEAL
                     NCSPKDIQAVLMGGHGDTMVPLPRYTTVSGIPVTEMIAADKLESIIQRTKVGGGELVN
                     LMGTSAWYAPGAAAAQMVEAIVRDQKRIFPCCAWLQGEYGQKDIYLGVPVKLGKKGIE
                     QIIELKLNTEEKQLLDGSAKAVKEVMNVLDGMQQPVK"
     gene            13215..>17062
                     /locus_tag="PKY09_09740"
     CDS             13215..>17062
                     /locus_tag="PKY09_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNB13181.1"
                     /translation="MKTFSKLIYTVFILAVTVFASKAQAPTNQDCPNAIPICNTTYST
                     VISYSGTGSIPNEINSGSSCLLSGERNDVWYTFTTPFFGNLNFTITPNNPADDYDWAV
                     YNLTTATCADIYTNPSIEVSCNYAPNLGCNGTTGPNNQTAGPCGGQNNPIIPVTPGQT
                     YVINVSNFSSTQSGYTIDFTASTGSIFDVFPPFLLTTSSLTCGSNSLTMTFNENITCN
                     SVQATDFVLTGPGGPYMITAVNSAGCTSGADYSKVYTVTFTPAIAVAGIYNFAVVSPV
                     QDLCGNLIAVPTNKSININGATVSAVHTDVSCFGDNDGSITLTPGGIGPFTYQWSPNV
                     SSSNTATNITAGTYTVTISPSNGACSALATVTVTEPPDMVLQNQNIVSAVCGASNGSI
                     ATTISGGTAPYQYQWSPSGGTGANASSLAPGNYTLTVIDNNNCDFTQTFNVPDNNSIN
                     ASVNTIQNVSCKNGIDGEITLSVTGVSASATYQWSNGATTKDLLNIPAGSYTVTVTDG
                     NCQTTLSNLTIIEPLTSVMSNPLATATTCGLNNGVITVNASGGTGALSYQWTPSGAGA
                     STQNLASGTYTITITDVNGCTAKRTVAIATSTSPVASVNYINQMVSCYNGSNGNASIS
                     VNSGTAPYTYLWSSGMGTTNSSNTLSNGNYNVTVTDQAGCTTVATVSILNAPQLILTV
                     GTVQHVKCFGQNTGSATFNGSGGTGALTFLWSPVGGNSNTASNLNAGTYTVLLTDANG
                     CTKNNSVTIQQPTAALNSSIAPTATNCGLNNGALVTSATGGTAPYTYLWSNGSANANI
                     SNLASGAYTLTVTDANLCVKTKTAMVAASTAPIINVTNVQNLNCANANNGSIAINVTS
                     GTQPYSYQWTNNVSNSNSASNLAAGSYVVTVTDNAGCVVSSSNNITSPVAITIQTLSQ
                     QNVKCFGNNTGSINIAASGGTGTLSYLWSNGNTSSAITTVTSGNYTVTVTDQNNCTKQ
                     QSFTITQPASALTSATTPVSTSCGLNNGSALTVPAGGTTPYKFLWSNGSNLAQINNLA
                     SGNYTVTVSDFYNCSLTQSAIILPSTAVTIQSTQQANVDCNGGSNGTASITASGGVAP
                     YNYLWTGGQTTASVQNLTAGNYQVTVSDNAGCTVLNSFIITQPSALTVVLPAAQTVCE
                     MQASTVAALPSGGTSPYYFLWSNGQTNDTLFATPSANTIFTLTVTDNNGCTSVASATI
                     NVFPQLTLNTFSPYSVCQNTLLSFTANVSGGNGQYQYNWNNGASTQQTFTQTYASSTS
                     VNLTATDGCNYSTSAIIPVNV"
BASE COUNT         5213 a         3352 c         3039 g         5458 t
ORIGIN      
        1 gctttgaggt taacctttgc ctgatacaaa taactcacgc ctgcataggt ataagtcagt
       61 ttatcaattg tagtcagtgt tttcagcaga aaattatcct gagtaaagtc accactaccg
      121 gtatgattca gattattgag tacggtactc attgccaaag cctcatcgtt atatgaaaga
      181 taaccgttag ttatttcata cttcttcaat gaaaaatgaa atggcgaaga ctcttcggct
      241 tttgcggggg tttctttttc aggtttggtg atatcccagt ttacttttcc gtctttcagt
      301 gctataagat gaatacgtgg ttgtattaat ccgaaatatt ttatctgcgt atttcctgat
      361 gtaataagtt tccaaaactc agctttaatc tgaacggatg aagctgaaat gagtgtatcg
      421 cctttaaaaa catcttttcc tacaatagaa acatcgttca atgctaaata tacagagggg
      481 aaactgcgta taaagcttaa atccaaatcc ttaaaatcga aagtcgcagt caggttttcg
      541 ttagctgcat ttttaatggc tgcaataatt ttatctttaa aaataaaggg tgcaaccagt
      601 gctgctgcga gcaacactac aaaggcaata atcgtaatac gaagaaattt tttcataacc
      661 ctgcggtttt tcagaaatta aacgaataaa aataaaaatt attgatgcct gcaaaactat
      721 tcaatagcaa tgaagtactt caggaaaaac aaatgaatta atcaacaaat tgttttaaaa
      781 agtccttgcg ggtaaaatta ttttattttt gacctgacaa taaaaactgt tcatgaattt
      841 attcagaaag aaaacagtaa atgaaataat gaaacgtgcc ggtgaagatg cctcagagca
      901 tggttcatta gcgaagcatt taggtgtgcg tgacctcact gcattgggca ttgccgccat
      961 tattggtgcg ggcatttttt ccaccatagg taatgcaagt gcgcaaggtg gcccaggtgt
     1021 aattttatta tttctgttta cagccattgc ctgtggtttc acagcttttg cctatgcaga
     1081 gtttgcatca ttagttccgg ttagtggaag cgcatatact tacagctatg ttgccttcgg
     1141 tgaatttata gcatggatta tcggttgggc attaataatg gagtatggca ttggaaacat
     1201 taccgttgcc atttcatgga gtgattattt aacaggacta ctcgatagta tgggcatgca
     1261 tctgccgttg tggatgacaa tggattacct cacagccgca aaaggattca gcaaagcagg
     1321 tgcgctgatg cttagtggaa gaacacttga aagtcttgat gccggtgtgc gcgatgccta
     1381 tttagcatgg cagggcgcgc caacaattgg tccgcttcat attgttgctg atttacctgc
     1441 tcttttaatt atactcatca ttacctggat tgtttacaga ggaataaaag aatcaaaaaa
     1501 tgcaggtaat gccatggtaa tcgtaaaact ttctgtagtg ctattggtaa ttgccgtagg
     1561 gttgttttat attaatacag ataactggca tccgttttta cccaacggca ttccgggagt
     1621 tttaaaaggt gtgtcggcag tattttttgc ttacatcggc tttgatgcaa ttagtacaac
     1681 ggcagaagaa tgtaaaaacc cacaacgtga tttacctcgc ggaatgatgt gggcaataat
     1741 aatatgcaca gtattatata ttgccatagc attagttctt acaggaatgg taaactacag
     1801 cacattggcc gtaggtgacc ctttggcata tgttttcagt gcattgaatt taaaatggat
     1861 gtcggcaatt attgctgtca gcgctattgt tgctatggca agtgttttat tggtttttca
     1921 attgggtcag ccacgcatct ggatgagcat gagcagagat ggtttgttgc cgaaaaaatt
     1981 tgctaccatt caccccaaat ataaaacacc ttcatttgcc tctatagtaa cagggtttgt
     2041 agtagccata cctgcacttt ttatgaacct taccatggtg acagaccttt gcagcattgg
     2101 aacattgttt gccttcgtat tggtttgtgc cggagtatta aaaatgcaaa taactcctaa
     2161 tgcacctcgc ggtagtttta aaacacctta tgtaaatgcg aaatttattt tccctgtaat
     2221 gctgatagtt gctatgatac tttcattgac atataataaa gaaggcgtta caaatttttt
     2281 aaagaacaca ccacaaccct atgctactga aaatctgatt tcatcattag gggcagaaag
     2341 aatcaaagat ttgatgacca ctgtttcttt aaaagatgct gatgcttttg cttcatctga
     2401 caatgatctg gccacttatc tttcgcaact aaaggaagat acttatcttc aaaaactaac
     2461 ggaatacgga ttgtcgcata aagaaattta cgaatcggga ttagaacttt tcaaacataa
     2521 aatacctaca tggatattta ttatttcatg tctgtatctt tgtgtcttaa catggaaata
     2581 caatttttcg cttattccac ttttagggtt ggtgtgttgc ctttatatga tgagtgaaat
     2641 ggggatatca aactggattg gattttcgat atggctcgct gtaggactag ttatttattt
     2701 tggttacagc tacaagaaca gtaagttaaa ttttttaaaa aaataaatat tatgccttca
     2761 atttttatta ccggtgcaac agcgggattc ggtcgagcca tagcattaaa atttgcacaa
     2821 caccattgga gcatcattat taacggaaga agaaccgaca gacttcgcac tttagaagaa
     2881 gaaataaaaa atcttggagc agatgttatc agtcttcctt ttgatgtacg caacgaacaa
     2941 caagttatgg aagctattga aaatcttcca aacaagtgga aacacattga tgcattggtt
     3001 aataatgcag gtcttgcagc aggagtaaat ttgattcagg atggcaaaac tgacgactgg
     3061 aacacaatga tagataccaa tgtaaaagga ttgctttatg taactaagaa tatcattcca
     3121 ttgatgataa aacaaaaaag tggacacatt atcaacatag gatccattgc cggaaaagat
     3181 gcttacatga aaggaaatgt ttattgtgcc accaagtatg cagttgatgc tttgacgaaa
     3241 agtatgcgaa ttgatttgct ggagcatgga ataaaagtca gtgcagtaaa tccgggtgcc
     3301 gctgaaacag aattttcttt ggttagactg aagggtgata ctgaaaaagc taatgctgtt
     3361 tataaaggtt ttcaaccatt agttgcggac gacattgcag aaattgtata ttttgttgca
     3421 acgcgtcctg cacatgtttg cataaatgat gttgttgtca caccaactgc acaggcaaac
     3481 acagctcatt tactacgtaa agccgactaa tcagtaacga gtatctgcta cttttaaaaa
     3541 aacttctgag atataattac gccaatgatt atcagaatag gttaaaccca tttcacgaag
     3601 tcttgacttt tcagggagct taaattgcgg aagccatcgt tcctgaggcg gtgcaaaccc
     3661 taatttatct tttcgattaa gaataacatc cggaataatt cctttcatgg cattacgata
     3721 aacatacttt gtggttgcat ttctgtaaat ctgattggca ggtaatgaaa aaacaaagtc
     3781 aaccagttta tgatacaaaa atggcagcct ggcttcaaca ccatgagcca tagaattact
     3841 gtcagaaaag tttaataact gttgaagatt ctgtaccatg tcattaaaaa gtgctgcttt
     3901 aaaagattga taaccgagtg tattatcctc aaatggtctt accgtttttg taagaaacat
     3961 ttttgtgagc tgccatgggc caataggaag tgaattttga tatctatcat tatatgcccg
     4021 cctctcattc atatattcca ttactctgaa acgaagcaac aagtcgttta aataatgaaa
     4081 acggtaatgc ccatatcctg ccagcatttc atcagcgcct tgcccatcaa gaataacttt
     4141 aatatgatga ttttttacct ccttgtaaag tagatattgc gctgctaccg atgttgagcc
     4201 aattggaatc tcgttatgat agacaatttt ttcaatttct tctagaattt ctttaggctc
     4261 aatctgttta tttatttgag ggaaatggaa tgaatctgta atatacttca tccatttacc
     4321 ttcatctttt acatcatcgt caaatcgggc tgagaataat ttataattaa ctttttctct
     4381 aagttgatgc attacacaca caattcccga ggaatcgagc cctccggaaa acgatgttgc
     4441 cacagggaca tcacttcgta accgcctgct tatagataaa aaaaacaatc ttctgaactc
     4501 ctccgcacat tcatgatcgt tcatggctac aatgttcctg tattgcaatc tgtaatactc
     4561 acggatatca attttgccat tctgcacaat agcataatga ccatgtggca gtttttttat
     4621 tccatcaaaa aaagtttcag tattataaat tgaaacatta ttctcaagga aattttccat
     4681 tttctcttta gaaactttct ttggaacctt tgctttgaat aatgctttta tttctgatgc
     4741 aaaaaccaac cgctcactat ccatatagta gcgcaaaggc ttttcaccaa aacggtctcg
     4801 tgctaaaaaa agctgttgca acttattgtc ccatattgca aaggcaaaca taccatcaaa
     4861 atcttcgaga catttctcac gcttattgtc aaatgctgca agaatcactt ccgtgtcgga
     4921 gtgcgatacg aactgatatc ctttcttttc aaggtcagcc tttagttcga gataattata
     4981 aatttcgcca ttgtaaacaa ttgtatatcg ccccagataa tgcattggct gccttgcttc
     5041 ttcagaaagg tcaatgatag ccaatcgcct gtgagcaaga ccaagatttt tctgttcatt
     5101 catccaaaca ccttcaccat cgctccctct gtaggaaatg gtgtctgaca tttgttttaa
     5161 tactgataaa tcaacgggtt tatgctgaaa attgataatg ccggctatac cgcacatata
     5221 tttgttctga tcaataaatt tgaaagtgca aaattagatt ttccacctta ccaaattgca
     5281 tgccgatgaa attaaatatg atgataggga tgcttgttca ggatggtaaa tgctctgtaa
     5341 atttgttcgg caaaaatcaa tcttgcatgc gtatgggtga atgtcatttt tgacagggcg
     5401 aacagctcgt tagatcgtgt ataaactttt tcagaaaaac cataagctcc acctgcaata
     5461 taaaccaact gcctgaaatt tttaagcctg agctgctcaa tttttgcagc aaactgaacc
     5521 gaagtgtatt ctttgccttt ttcgtccaga agaattacat agtcatttgg agataactct
     5581 ttaaaaatat tatcagcttc aacatccatt gccgctgtaa ggtttttgtt gtttttagcc
     5641 tctttgaggt actttatttc aaaaccgtta aacctttcca ccctattagc atattctact
     5701 atagcagctt cggcaaaact gtccttattc tttccaatca tcagcaattt aatatgcata
     5761 atttacgtta ttcaacaatg ttatattaca aaaatccgaa atttagtttt gaaagtaaat
     5821 ttttatgccc taatattgtt tcggatttac ttttaagtgt ttaattagta ttgtaagata
     5881 tatgccaaaa tgcattcata agtttttcat tcccttgcgc gcttggcttg atttttgtca
     5941 gttcacaaac ataatttatc aatgatgaaa aaaaccatca agcttcttct ttcagtcctg
     6001 ctgatagcta ccgttgtaaa ggcagctgat gttttcgatg atattggcgc agccattcgt
     6061 tcgggcgatg ccaagcagat tgcacgtttt tttaacaaca atgttgattt gacaatattt
     6121 aatcaggaag aagtttacag taaagcccag gcagaaatgg tgcttaaaga tttcttcagc
     6181 aaaaacgcac caaaaagttt tactatcatc cataagggtg tttccaaaga aggtgcacgc
     6241 tatgcaattg gaacattcac aacaacacaa ggtcagaata tcagaaccta tttctttgta
     6301 aaggaaagtg gtggcactgc ttcaattcag gagttacgct tcgaaaaaga ataaaaatat
     6361 cttccccaca gttttttata gccttattca tacttacata cggctaattc attcttagta
     6421 tttcaaaaaa attgattctt acatgattct ttgaatcaaa aagctgaatt ttgcggcaga
     6481 aataattatc atggatttag atcagtatat attattatcg cttcaggaag atgtaggtcc
     6541 tggcgatttc agttctctga gctgcattcc tgcacatgca agcagcaagg ccaaactaat
     6601 tattaaagat aaaggaatac ttgcaggtgt tgaacttgca gaacgcatat ttaaaactgt
     6661 tgattcctca cttcaaatca atattcatat aaaagatgga cagcatgttg ataaaggcga
     6721 aattggattt accatagaag gaagtgttct ttctattctg aagtctgaac gattagttct
     6781 caactgtatg cagcgtatga gcggcattgc ctccactact cacttatatg ccgaacaagt
     6841 gaaacataca aaagcaaaaa ttttagatac aagaaaaaca acacctctga atcgttatat
     6901 tgaaaaatgg gctgtacgca ttggtggtgg gcacaatcat cggtttggac tgagcgatat
     6961 gattatgatt aaagacaatc atgtagatta tgcaggagga ataaaacagg caattgatgc
     7021 ggcaaatgtt ttcaggaaaa ccagaaacct gaatattgcc attgaaatag aaacacgtaa
     7081 tttaaacgaa gtcagtcagg tgttgagcac cggtggtgta gaaagaatta tgctcgacaa
     7141 ttttcaacca actttattaa aagaagctgt tgctttgata ggtggtcgtt ttgaaacaga
     7201 agcttccggt ggcattatac tacaaaacat aaaagaatat gctgaaacag gagttgacta
     7261 tatttctgta ggtgcattaa cacattcttt tataagcatg gacatgagtt taaaagcaac
     7321 ccgatgaaac gcaggttgct cgaatactta tctctcatta taaactatac acgtagaatt
     7381 acactgcccg gctttcagaa aattccaatt tatgatgtgg gcaaattttt tcttcgtgag
     7441 ttgtttaaag gcgatttaca aattagagca agagcaattg cgttttcatt ttttttagcc
     7501 attttcccgg ccatcatttt tctttttacc ttaatccctt atgttccaat agatggtttt
     7561 caagaaagat tattaagtat tttacaatcg ctgttacctt acaacaccta tgacacagca
     7621 tataagacaa ttgaagaaat tattgtaagg caaaacggag gactgctttc aattggtttt
     7681 attctggctt tatttgtttc taccaatggt gtgtttgcac tgatagaagg tttcaataaa
     7741 accatcatgc atgaaaaacg taaaggctgg aaacagagat tagttgcact ctatctaacc
     7801 attcttttag caactttatt gattattgca attttactca ttatcataac tgaggttatt
     7861 ctacattatt tcaataatca tattcttaac ctgaatcaaa attctgttta tctcttgctt
     7921 gccggtaaat atctaatcct gattttgctt tgtttcacag ccatatcatc actttacttt
     7981 tttggtcctt ccggaggaag taaaaaatgg catttctttt ctccaggcag tattctggca
     8041 acaatactta ttgctgttac atccattgca ttcaactttt ttattctgaa ttttggacaa
     8101 tacaataaaa tttacggttc tatcggaaca ttgatagtaa tattaattta cctgaacttt
     8161 aactgcattc aattactaat aggatttgaa ctgaacaatg ccattgaaaa agcaaaattc
     8221 aaacctaata cagaagaaat taaatcttaa aagacttcct ttctttcggg ctgactgata
     8281 gtaaagttta atggtttttt tactttatcc tgaagtaatg caaggtcaat tacctcaccc
     8341 attttactta cgtaatgaaa tctcagccct ttaagatact cactcttgat gtctttaata
     8401 tctttctcat tgtcttcaca aagaattatg tctgttattc ctgcgcgttt tgcagcaaga
     8461 attttctctt taataccacc aacaggcaac acttttccgc gcaaagtaat ttcacctgtc
     8521 atggctaatt tcaatttaac tttacgttgt gtaaatgctg atgcaaggga agtgagcatg
     8581 gtaatgccgg cagaaggacc atcttttgga gtagctcctt cgggaacgtg aatatgaatg
     8641 tcccatctat cgaacacatg ataatcaata ccaaaatctg ctgcatgtgc ctttaaatat
     8701 gctaaggcaa tttgtgcgga ttctttcatt acttcaccta agtttccggt aagcaacaaa
     8761 cgtcctttac ccctgctcaa actcgtttca ataaataaaa tatcgccacc gactgatgtc
     8821 catgcaagtc ctgttacaac acctgcaaca ctattgtcgg caaatctgtc gcgcaaaaat
     8881 attggtgctc ctaatgcttt ttccactgca tctttatcaa ccttcttatc atatttttca
     8941 ttcagtgcaa cgcgctttgc aatgcttctg attactgatg ccaggttgcg ttcaagacta
     9001 cgaacaccac tttcgctggt ataaaattca ataatatatt caattacact gttattgaaa
     9061 gtagcatggt gtttctttag tccatgcgca tccaactgtt ttgggataag atgttttact
     9121 gcaatctgaa ttttttcttc aacagtgtag ccattgattt ctattatttc catcctgtca
     9181 cgcagtgcag gctgtattga actcaagctg tttgccgtag caataaacag aacgtttgac
     9241 aaatcataat cgagctcaat gtaattatca taaaaggcat tattctgttc tgggtccaac
     9301 acttcaagca gtgctgagct tggatcacca tgaaaatcgt tggtgagttt atcaatttca
     9361 tcaagcacaa aaacagggtt tgatgatttt gcctttttta tattctgaat aattctgccg
     9421 ggcatggcac caatataggt cttgcgatgt cctctgattt ctgcttcatc acgtagtccg
     9481 cctaaagaaa cacgaacata ttttctacct aatgcttttg ctactgactt accaagagag
     9541 gttttaccaa ctcccggagg accataaaga cacattattg gtgacttcat gtctttcttt
     9601 agttttaaaa ctgccagata ctcaagtatg cgtgttttta ctttctctaa tccgtaatga
     9661 tctttgtcca gaactttttg tgcatttttc aggttaaaat tatcttcagt gaattcaccc
     9721 caaggcaatt cgagtaataa atccagataa tttaaagtta cactgtattc tgctgctgca
     9781 ggatttatac gtcctaatct ttcaatttct ttattgaaag tttctgctac atgcttattc
     9841 catttctttg acttggctcg tgtctttaat tgctgcaatt cctgatcagg tgtgctgcca
     9901 cccaattctt cctgaatagt ttttaattgt tgatttaaaa aatattctcg ttgttgttta
     9961 tctaaatcta cttttacttt attctgaatc tgcgatttca actcaagcat ctgcaactcc
    10021 tgtgtgaggt actcaagcac ttttaaagca cgttgtttaa tatcagtcat ttcaagcagg
    10081 tcttgctttt tggaaacttc catctgcata ttacttgaaa taaaatttat cagaaaagca
    10141 gggctttcaa tgttgcgaac agcaaatccg gcctcactag gaatttgtgg agagtgttga
    10201 ataatctgca acgaaatatc ttttaaagaa tctactgttg ctttaaattc tttctcctta
    10261 agtgcggcaa caatatcgtt tagtggttca actttagctt tcagataggg ctcactctgt
    10321 gtcatttcaa gtagtgaaaa cctgcgtttg ccctgaatga ttatggtagt atttccatcg
    10381 ggtagtctca acatttttat aacactggct accgtaccga ttttattcaa atcattaaag
    10441 ccgggatctt caatattggt gtctttttga gaaacaacac caattatttt attgctcttg
    10501 taggcatctt ttatgagttt aattgatttg tcgcggccta ccgtaattgg aatcactaca
    10561 cccggaaaaa gtacggtatt gcgtaatggc agaattgaca attcgtcagg tatgctttct
    10621 ttattaagtt gttcttcatc ttctgatgaa agtaatggta ttaactcagg ttcatcgtca
    10681 tttatgccgc caatgagtgt gtgtttaata tcttcgggat tgaacatagt tgcgtcaagt
    10741 tgtcagccaa caggctgcta ttagtttaaa atataatatt gtcaatgctt atgccattaa
    10801 ttttttggca gtatgagttc aagcatgtta acatggttaa taatgtcttg ttctttttta
    10861 tcaaaaaaca aatctcttcg tttataaact tctgcagcaa gttcaagtgc tttatcaagc
    10921 tgaaagaagc tttcatcatc aaatcctccc caggcaaaag agggtataaa tttaggaggg
    10981 aaaccactgc caaaaatatt ggcgcaaatt ccggtcactg tgcccgtatt aaacatggta
    11041 ttgatagcag ccttactatg atcgcccatc ataagtccgc aaaactgcaa gcctgtattg
    11101 atataatctt ttttatggta attatatgct tttactatac cataattatt tttcaggtta
    11161 gagttattgg tatcggctcc aaggttgcac cactctgcaa ttaccgcatt accaagaaag
    11221 ccatcatgtg ccttattgga ataaccaaaa attactgaat tgtttacttc gccaccaact
    11281 ttacaatggg ggccaatggt tgtggcgcca taaattttag cacccatttt taatgaagca
    11341 tgctcgccaa gcgcaaaagg tcctctaatc atgcaacctt ccattatttc agaatcacga
    11401 ccaatataaa cagggcctgc tgaagcattt attatgctgc acatcacttt ggctccacct
    11461 tctataaaaa cctgctgttc gttaattatt gtatttgaag aatcatagtc agcacttttt
    11521 cttccttctg taattaattg aaagtcgctg ttgattgcct tatcattgaa acgaaaaata
    11581 tcccatggat atttaatcac gttaacttca agacctgttt caaatgcctc actataactg
    11641 acatcttcca atgattcgaa ttgttgttgt tcggtacaaa cagcaattac agtattacct
    11701 ttcactaatg cctgacctgt tttcaatgtt tttacttcat tgaaaagtgc tttatcaatg
    11761 agtacagatg cattagcaat caggttaact ttttctttcg ttaaagaata ttttgtggag
    11821 agatattttt cagtcagcca tgaacatggt gtaccactaa ggtgatgcca tttttcggta
    11881 atggttaaaa taccgcaacg aatctctgcc acgggtctta taaatgtaaa tgggagcaaa
    11941 tcaactcgat tgctatcagc aagtataatg ttcattgaga attaatgggt taaaaattat
    12001 ttattacaat agtctgaaac tgtaatgcat ttctgcgaat gtagctaaaa tatcagttca
    12061 tcagattaag taattattaa aaaacaaatg ccgcctgtca acaagcggca tttaatagtt
    12121 ttatcggaag aaatttactt tacaggttgc tgcataccat caagcacgtt cataacttct
    12181 tttacagctt ttgcactgcc atcgagtaat tgtttttctt ctgtattgag ttttagttca
    12241 ataatctgtt caataccttt tttacctaat ttaaccggaa caccgagata aatatctttc
    12301 tgtccatatt cgccctgcaa ccatgcgcaa catgggaaaa tacgtttttg atctcttaca
    12361 attgcttcta ccatttgagc tgcagcagca ccgggtgcat accaggcaga ggtgcccatc
    12421 aggttaacca attcaccacc accaacttta gttctttgaa taatactttc taatttatcg
    12481 gcagctatca tttctgttac aggaatacca gaaactgttg tgtagcgtgg taatggaacc
    12541 atggtatcac catgaccacc cattaatact gcttgaatat cttttggaga acaatttaat
    12601 gcctcagcta aaaaagctct atatcgtgca gtgtccaaaa tacctgccat accgaaaact
    12661 ctcttagaat cgaacccaga tgtcaggtaa gcacaatatg tcattacatc aagtggattt
    12721 gaaacaacta taatgatggt atttggagaa tgtttaacaa tattttcagt cacagaacgt
    12781 acaatacctg cattggtagc gattaaatca tcgcgactca tacccggttt acgtggaagg
    12841 cctgatgtaa ttacaaccac atcagaacct gctgtagcag cataatcgtt agttgaacct
    12901 tttattcttg tatcataaag attgattgga gctgtttgcc acatatccaa tgattttcct
    12961 tcagcagtac cttctttaat atcgagcaat acaacttcgt taaccaattc gcggtgagca
    13021 atcacatttg cacaggtagt tcctacattt cctgcaccta caacagtaac tttcattttt
    13081 tttacttatt taaaattttt cagagggcga aattatctaa aataatattt ccgagtggca
    13141 tttgttaaaa aatttaatgt tacatttttt tggtgaatgt gtttgattat taagtgaaat
    13201 tgtatatttg tcgtgtgaaa accttttcca aattaattta tacagtattc atcttagcgg
    13261 ttaccgtttt tgcttctaag gcacaagctc ctacaaatca ggactgtccg aatgcaattc
    13321 caatctgtaa cactacctac tccactgtaa tttcatacag cggaacagga agtattccta
    13381 atgaaattaa tagtggcagc tcctgtctgt taagtggcga acgaaatgat gtatggtaca
    13441 catttacgac accatttttc ggaaatctta attttacaat tacaccgaac aatcctgctg
    13501 acgattatga ctgggcagtt tacaacctta ctactgccac ttgtgccgat atctacacaa
    13561 atccgtctat agaagtcagt tgcaattatg cacctaacct tggttgcaac ggaactacag
    13621 gccctaataa tcagactgcc ggtccatgtg gcggacaaaa taatccaatc atacccgtta
    13681 caccgggtca gacctatgta attaatgtaa gtaatttttc ttccacacag tcgggctata
    13741 ccattgattt tacagcgtca acaggaagta tttttgatgt ttttccacct tttttattaa
    13801 ccacttcatc attaacatgc ggttcgaatt cattgacaat gacatttaat gaaaacataa
    13861 catgcaactc ggtacaggca acagattttg tattgacagg ccccggtgga ccttacatga
    13921 ttacagcagt taattctgcc ggctgtacca gtggcgcaga ttacagtaaa gtttacacag
    13981 taacttttac ccctgctatt gcagttgccg gtatttacaa ttttgcggtt gtaagtccgg
    14041 ttcaggattt atgtggcaac ctgattgctg ttccaaccaa taaatcaatt aacattaatg
    14101 gtgcaacagt ttcggccgta catactgacg tttcttgttt tggcgataat gacggaagta
    14161 ttacactgac tcccggtggt attggacctt ttacttatca atggtcacct aatgtgagca
    14221 gcagcaatac tgcaacaaac attacggcag gaacttatac tgtaacaata tcaccatcta
    14281 atggtgcatg tagtgcattg gccacagtaa cggtaacaga accacctgat atggttttac
    14341 aaaaccaaaa tattgtctct gctgtctgtg gagcttccaa tggttctatt gcaacaacaa
    14401 tttccggtgg aacagcacct tatcaatatc aatggtcgcc atcaggtggc acaggtgcta
    14461 atgcttcatc attagcaccg ggcaattata cgcttacggt tattgataac aacaattgtg
    14521 attttacaca gacatttaat gtccctgaca ataatagcat taatgcatca gtaaacacga
    14581 ttcaaaatgt aagttgcaag aacggcattg atggtgaaat aacattgtct gttacaggtg
    14641 tttctgcaag tgctacttac caatggtcga atggagcaac aacaaaagat ttgctgaata
    14701 tacctgcagg cagctatacc gtaactgtaa cagatggtaa ttgtcagact acactttcca
    14761 acctcaccat tatagaacct cttacttcgg taatgtcgaa cccgttggct actgccacta
    14821 catgtggact aaataatgga gtaatcactg taaatgcttc tggcggaacc ggggctctca
    14881 gttatcaatg gactccttca ggcgcaggtg cttcaacaca aaatttagct tcaggaactt
    14941 atacaattac tattactgat gtcaatggat gtacagcaaa aagaacggtt gctatagcaa
    15001 cttcaacatc acctgttgcc tctgtaaatt atattaatca aatggtttcc tgttataatg
    15061 gcagcaatgg gaatgcaagc atttctgtca atagcggaac cgctccttat acttatctgt
    15121 ggtcgtcagg aatgggtaca accaattcaa gcaatacact ttcgaatgga aactataatg
    15181 taacggtaac agatcaggca ggctgtacaa cagttgctac cgttagtatt ttgaatgcac
    15241 cacagttgat actaactgtc ggcaccgttc aacatgtaaa atgttttggg caaaacacag
    15301 ggagtgcaac ttttaatggt tcgggaggaa ccggagcact caccttcctt tggtcgccag
    15361 tggggggaaa tagcaataca gccagcaact taaatgctgg cacctacact gttttattga
    15421 ccgatgcaaa tggatgtacc aagaataatt cagttaccat acagcaacca acagcagcac
    15481 taaacagcag tattgcacca actgcaacca actgcggcct taataatgga gcattggtta
    15541 cctctgctac cggaggcact gctccatata cctacctgtg gtcaaacgga tcagctaatg
    15601 caaatatttc aaatcttgca tcaggagcct acacgctaac agttacagat gcaaacctgt
    15661 gtgtaaaaac caaaactgca atggttgctg cttcaacagc acccataata aatgtaacta
    15721 acgtacaaaa cctaaactgc gcaaatgcca acaacggtag catagccatc aatgttacaa
    15781 gtggaactca accctacagc tatcaatgga caaacaatgt ttccaacagc aacagtgctt
    15841 ccaatctggc agcaggaagt tatgttgtta cagttacaga caatgccggc tgtgtagttt
    15901 cttcttcaaa taatattact tcaccggttg caataacaat tcaaacttta agtcaacaaa
    15961 atgtgaaatg ttttggcaac aataccggta gtattaacat tgcagccagt ggtggtaccg
    16021 gcacactttc ttacttatgg tcaaatggca atacctcatc agcaattact actgtaacat
    16081 caggcaatta tacggtcact gtaactgatc agaacaattg cacaaaacaa caatctttta
    16141 ctataacaca acccgcttct gcattaactt ctgcaacaac acccgtcagt acttcctgcg
    16201 gacttaacaa cggttctgct cttactgttc ctgccggtgg cacaactcca tataagtttc
    16261 tctggtcaaa cggatcgaat ttggcacaaa tcaacaacct tgcctccgga aattatacag
    16321 ttacagtttc agatttttac aattgcagtc tgacacaatc tgccatcata cttccatcta
    16381 cggcagttac cattcaaagt acacaacagg ccaatgttga ttgtaatgga ggtagcaatg
    16441 gcacagcttc gattacagca tccggtggtg tagcaccata taattatcta tggacaggtg
    16501 gtcagacaac agcttctgta caaaatttaa cagcaggaaa ttatcaggta actgttagtg
    16561 acaatgctgg ctgcacagta ttaaattctt ttatcattac acaaccttca gcacttactg
    16621 ttgtattacc ggcagcacaa accgtgtgcg aaatgcaagc ttcaacagtt gctgcattac
    16681 catcaggtgg aacttctcct tattactttc tatggagtaa tggccagact aatgacacgc
    16741 tctttgcaac accttctgcc aatactattt ttactttaac tgttaccgat aataatggct
    16801 gtacttctgt tgcatctgca acgattaatg tttttccaca gttaacttta aatacattta
    16861 gcccctactc tgtttgtcaa aacacattgt taagttttac tgcaaatgtt tcaggcggta
    16921 atggacagta tcaatacaac tggaacaatg gcgcatcaac acaacaaact tttacacaaa
    16981 cctatgcttc atcaacatcg gtaaacctta ctgctactga tggatgtaat tattcaactt
    17041 ctgcaatcat ccctgtcaat gt
//
LOCUS       DAOQNP010000071        16080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1287_length_16080_cov_7.878502, whole genome shotgun sequence.
ACCESSION   DAOQNP010000071 DAOQNP010000000
VERSION     DAOQNP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 16080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16080
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_16080_cov_7.878502"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PKY09_09745"
     CDS             complement(<1..179)
                     /locus_tag="PKY09_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNB13182.1"
                     /translation="MSNGSKILGALLIGAAAGAILGVLFAPDKGEETRKKIRKKGEDI
                     LDDIKRKINEANDEL"
     gene            complement(274..624)
                     /locus_tag="PKY09_09750"
     CDS             complement(274..624)
                     /locus_tag="PKY09_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13183.1"
                     /translation="MYFIEINVNEMSTDRLQQLEAMYADNPQELFVLYALALEHVKLG
                     NEKQSVKYFEQVLSVNRSYLPVFYQYASFLQDKDILKAKLLCQEGILLAGNQNKLKTK
                     TELLSLLEQLNDSD"
     gene            672..1409
                     /locus_tag="PKY09_09755"
     CDS             672..1409
                     /locus_tag="PKY09_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNB13184.1"
                     /translation="MKILVCISHVPDTTTKISFTADNKSIATSGIQYIINPYDEIALT
                     RALELTEKSGGTVTVINVGDPSTDATLRKALAIGATDAVRIDANPANASFVATQIADY
                     ASKNSFDLILTGRESIDYNGSQVAAMVGELLNIPSVSIVKSLEVADKTVTLEREIEGG
                     KEVISCPTPLVASATEGMAEPRIPNMRGIMSARTKPLQVVAAIATETYQQLAGYDKPA
                     PRTQVKLIDASNVKALVDALHTEAKVI"
     gene            1434..2411
                     /locus_tag="PKY09_09760"
     CDS             1434..2411
                     /locus_tag="PKY09_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNB13185.1"
                     /translation="MNVLVYIETAEGKLKKSATEVLSYGKVIAKNSGGTLTALAIGDL
                     TTDEQGKAGRFGADKVLHVTNDKLKQFINQAYASVVAEAAKQNNASVVIISNTFNGKA
                     MAPRVAVKLKAGMVDGAVGLPDLSNGFKVRTSAFSGKAFADIELTSAIKVITVAPNSF
                     KAEENQSAGTVETFSASVADSDFTAMVKEIVKASDKISLPEAEIVVSAGRGLKGPENW
                     GMIEELATLLGAATACSKPVSDAHWRPHSEHVGQTGLAISPNLYIAIGISGAIQHLAG
                     VSSSKTIVVINKDPEAPFFKAADYGIVGDAFEVVPKLIDEVKKFKAANG"
     gene            2445..3065
                     /locus_tag="PKY09_09765"
     CDS             2445..3065
                     /locus_tag="PKY09_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNB13186.1"
                     /translation="MKKIRLDIFGLSYSQAQSGAYALVLNEQGGTRRLPIIIGGYEAQ
                     AIAVELENMTPSRPLTHDLFKNFSISFGIHIDEVTIYNLKEGVFYSKIVCNNGEKQLE
                     IDARTSDAIALAVRFNCPIYTFDFILAQAGVEMDEDLGTSATSSEMPQIEKPAPTPRK
                     RTKSIGSNDFSHLSTEELNEALINAIDNEDYEAASKIRDELTRRKK"
     gene            complement(3105..4034)
                     /locus_tag="PKY09_09770"
     CDS             complement(3105..4034)
                     /locus_tag="PKY09_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNB13187.1"
                     /translation="MKISNEVKVGIAFTLTIAGFFWGMNFLKGTDLFSTSNKYYVVYD
                     DVNGIVKSNPVIMNGFKIGQVDKIVYAEDKSGRLIVTLRVDDNVFIPKSSNAAIVSSD
                     LLGSKAIELEMSEDKTPAKNGDTLVGNVQSGLTDQIKPLKNKAEGLVTSLDSLSYALT
                     QLINERSRNSLNNIFANLESTTEGLDKMVNNSNSKLNSALSNIDGVAGNLNNKQTDIN
                     GILKNVNSLSDSLSRLQLNLTIEKLNKTIAELNSVLGKIDNSQGSLGKLVNDDSLYNN
                     LNNASANLDKLLIDLKANPGRYVNISVFGRKNK"
     gene            complement(4046..5443)
                     /locus_tag="PKY09_09775"
     CDS             complement(4046..5443)
                     /locus_tag="PKY09_09775"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNB13188.1"
                     /translation="MLFIYKKIKIRVFFTLSLLFLICLSASNGTYKEPYRIKTIVIDP
                     GHGGHDSGCLGASSKEKQIALEVALKLGEMITQKYPDVKVIYTRKTDEFVELHERAAI
                     ANRNHAELFICIHCNSGSPAAFGAETYVMGLHKSEDNLNVAKRENAAVLFEKDYKQKY
                     DGFDPNSPEANIVFTLYQNAYLTQSLKFASAVQQQFEVYAGRYNRGVKQAGFLVLYKT
                     AMPSVLIETGFLTNRTEEKYLLTEKGQNAIATSIFRAFMEYKLDMETPGAKIPQGGAE
                     NDLNKNVVEVKNSDNQPAKETTQPQKKVEENESNSTIPNSNKEDQDVKKDAAKNNADN
                     AGKNKETQPKENNKTESPVKPVAPDEIDLPAEEKKKAVNTEKLPDFFYTVQLAALPDA
                     SKEELTRFEDLTDIKTFKAENGMIRVCQGVYTTHAKALAAQSQVRAKGYKDAFVTPYY
                     KGKRISNKEAEAIKK"
     gene            5573..8068
                     /locus_tag="PKY09_09780"
     CDS             5573..8068
                     /locus_tag="PKY09_09780"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNB13189.1"
                     /translation="MWCLAVIVFSTENIHAQTLPKDSLSTDTLVKDTLKTGVSNDIKS
                     KVHYVADDTIEFDVEHERVFMYEKAQVNYENIELKAAYIEVDWNTKNVFSAGLRDSTG
                     KLYGMPVFKEGDDEFKADTMRYNFGTKKGKVYHVVTQQGDGFIHGAIVKKVEESSYIR
                     YGKYTTCSLDTPHYYIAANKLKVIPNNKIITGPAYLIIGDVPAPAAIPFGLFPNTKGR
                     SSGILFPAYGESNALGFFFKNGGYYFGISDHVDLQLTADIYTLGSWAVKATSRYNNRY
                     HYNGNLSFLYSIIKVSEKDLPDYSRSKEFFINWSHLQDPKARPNGIFSASVRAGSNDY
                     YTRNISNASNYLTNTFNSSISYSRSFFNKQLNFSSAITHSQNTQTRIVYLTAPSINLS
                     MNTLYPFRKKEQEGTLAWYEKISVGYSSTLQNQLQNPDSLFFKNTTLQQMKNGMQHNI
                     PISASFKVMKYFNLTPAINFNERWYIETYKQRFNADSNRIITDTVKGFNAAHEYNGSL
                     SLNTRIYGMKQFKKGKIAAIRHVLSPTLSYMYRPDFGENKYGYYKTVQTDSLGNSRRY
                     SIYENTLYGGPSLGKSNVLSFSLDNNLEMKTRQYTDTAVNIKKIKILESFGLSASYNL
                     SADSFNLSPIGMSARTTLFDMVSLTASGTFDPYLMNDQGIRIAKYSLAENGKIARLTS
                     ASGSVGFNLNRQKGNKKSLKGTVEQVKEINDHPDDYVDFSVPYNLSVNYSIAYSRPTN
                     EKGNLSQIVGFNGDISLTEHWKITFNSGYDFQTKKQSYTSLGFYRDLHCWEMRLNWVP
                     FGYQANYYFQINVKSSVLQDLKLTKKNDRYDNF"
     gene            complement(8071..8985)
                     /locus_tag="PKY09_09785"
     CDS             complement(8071..8985)
                     /locus_tag="PKY09_09785"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNB13190.1"
                     /translation="MNFEVTILGSSSATPIHQGHPTAQVLNIRERFFLIDCGEGTQGQ
                     FLKYKFKLNKLSHIFISHLHGDHYLGLVGLLSTMHLQGRTTEIHIYGQPELMDVIELQ
                     LRLSQTRLRYQLIFHPVQHYAPSIIYEDDDMLIKSIILNHRIPCTGYHFSEKPKPRKL
                     IIAKLQQHNIPFSFYGRLKDGFDYEDDEGKIIPNAELTISSTAPRSYAFCSDTCYDES
                     IIDEIKGVNLLYHEATFMNDLAERAKTTFHSTSLQAATIAKKAGVDKLIVGHFSARYR
                     DLKPLLDEARTVFKNTELAIEGHKFSVE"
     gene            complement(8985..9755)
                     /locus_tag="PKY09_09790"
     CDS             complement(8985..9755)
                     /locus_tag="PKY09_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13191.1"
                     /translation="MIKKLLFSLMFISMSGIAVSQCTPDPTIIDPGVYPDSATGLSNG
                     MVGVAYNQVIQVRTFFDTTATINIGGVPFNANFELDSITILGVSGLPPGLTYACNPGT
                     CQFLASSNGCLLLSGTPVTAGVYPITVDIIARGKITNLGGISYGQPMSVTYYTITIDT
                     NSGVIELLPKDKLSMSQNLPNPFKNKSVIYFNSPVNTKIDFKIYNILGKNIFSETISA
                     KRGVNTYTINAENLSAGIYMYSINSGTANITRRMVISK"
     gene            complement(9773..10129)
                     /locus_tag="PKY09_09795"
     CDS             complement(9773..10129)
                     /locus_tag="PKY09_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNB13192.1"
                     /translation="MHYTLDKKEKYAVLKLHEQKLNTLISAELKSELLILNSQGYIHI
                     VLDLSDTQYCDSSGLSAILVGNRICRNANGNFIVTEISDPVKKLINISQLDSVLTIVP
                     KIDEALAQINKAAAVS"
     gene            complement(10212..11141)
                     /locus_tag="PKY09_09800"
     CDS             complement(10212..11141)
                     /locus_tag="PKY09_09800"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNB13193.1"
                     /translation="MGKLSVKHLIGIKNLNPEDINLILDTAQTFKEVINRPIKKVPSM
                     RDVTVVNLFFENSTRTRLSFELAEKRLSADVLNFTASSSSVSKGETLIDTVNNILAMK
                     VDMVVMRHPKPGAALFLSQKVNSVIVNAGDGTHEHPTQALLDAFSIRQKLGTLKGKKV
                     VIVGDILHSRVALSNIYCLQKSGAEVMVCGPATLIPKYIEQLGVKVETNLINALNWCD
                     VANVLRIQLERQDIKYFPSLREYIMQYGITLDILKKLQKKIVIMHPGPINRGVEITTE
                     VADSDQSIILDQVENGVAVRMAVLYLLAGQPVG"
     gene            complement(11122..11691)
                     /gene="pyrR"
                     /locus_tag="PKY09_09805"
     CDS             complement(11122..11691)
                     /gene="pyrR"
                     /locus_tag="PKY09_09805"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNB13194.1"
                     /translation="MTHLILAGKQLDITIERLCHQLIENHGNFKDTCIIGLQSKGVPL
                     SRRICKRLSEFLPESNILHGALDITFFRDDYRRNEKLLVPSRTEIDFIVEGKKVVLVD
                     DVLFTGRTIRAAMDALMAFGRPAKVELLVLIDRRFSRQFPIEPDYTGQAIDTTVNDKV
                     QVEWKESGSKDGVWLVSEIKSKTNGKAKR"
     gene            complement(11807..12646)
                     /locus_tag="PKY09_09810"
     CDS             complement(11807..12646)
                     /locus_tag="PKY09_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HNB13195.1"
                     /translation="MTNFIPVRVAVLDLYNGEPNEGMRSIRTILDRFSKFIECSFFDV
                     RQNNEVPDLGYDIYISSGGPGCPLPAGEAWEEPFYALWDKLIEHNAIAENKKKYAFLI
                     CHSFQMMAHHLGFGIVSERHSPSFGIFPIHKTEDGNNDRIIGRLPEPFFAVDSREWQV
                     TTPDIDKIVRSGGKLLCLEKIRPHVPYERAIMGLRFSDEILGVQFHPEADAEGMYRYF
                     DRADKKEKIIEHYGSEKYYDMIESLSDPNKIELTHHAIIPCFLADAIEHLNPQLRMQK
                     ATV"
     gene            complement(12646..13791)
                     /locus_tag="PKY09_09815"
     CDS             complement(12646..13791)
                     /locus_tag="PKY09_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HNB13196.1"
                     /translation="MAYINKKFRLPQFTIGVEEEFQVIDPKTRELRSHMHQIVEGGKV
                     ILKEQVKAEMHQSVVEVGTNICKDVSEARKEVTYLRQMVAKLAHKQGLVIAAAGTHPF
                     SRWQDQLITDHPRYEEIIREMQDAARSNLIFGLHVHVGIDDREIGIRLMNQARYFLPH
                     IFALSTNSPFWEGRNTGFKSFRTKVFDKFPRTGIPDEFSSAAEYDAYIQLLVKTNCMD
                     NAKKVWWDIRLHPFFNTIEFRICDIPMRIEETITLAAIMQGIIAKLYLLMQFNLSFRT
                     YSRALINENKWRAARYGIDDKLIDFGKEKEVATKVLIEEITEFIDDVVGDLGSRKEVN
                     HVFNMMKKGTGADRQLAVFEKTGDLKKVVDYIISETNIDIPVKKEKK"
     gene            complement(13809..14807)
                     /locus_tag="PKY09_09820"
     CDS             complement(13809..14807)
                     /locus_tag="PKY09_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13197.1"
                     /translation="MKKIGVLFGQENSFPPAVVERINSKNISGIMAESVQVDKVVQGM
                     PMDYAVIVDRISHDVPFYRGMLKNAAICGTAVLNNPFWNSADEKFFNNCLATKIGVPV
                     PKTVLLPSNVHPPDTNENSFRNLKMPLDWDNIFKYIGFPAYMKPFAGGGWKNVYKCND
                     ATDFYNKHQETGQLVMLLQEEIVFDQYFRCYCIGGKRIKIMRYEPRNPHHLRYVVDGP
                     APEQRILDVVEEYTFKLCNALGYDINTVEFAIRDGVPYAIDFCNPAPDADRHSVGEEN
                     FNWVIEAVADMCIEKALAHREGQDNLTWGAFVKHAAQGQIVNKQNMDALMESYTVK"
     gene            15169..15969
                     /gene="dapB"
                     /locus_tag="PKY09_09825"
                     /pseudo
     CDS             15169..15969
                     /gene="dapB"
                     /locus_tag="PKY09_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518782.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
BASE COUNT         4991 a         3158 c         2936 g         4995 t
ORIGIN      
        1 tcaagttcat cattagcttc attgattttt cgtttgatat catcaaggat atcttctcct
       61 ttttttctga tttttttgcg tgtctcctca cctttatcgg gggcaaataa tactccaagg
      121 atagcaccgg cagcagcacc tatcagcaat gcgcctaaaa tttttgaacc gttagacata
      181 gcgttttatt tttaataatt attctacaaa gataatcacc ctttacagtt tattgatgaa
      241 tattttagca aaacacaatt taagggtaat tgttcagtcg ctatcattca attgttctaa
      301 cagtgaaagc agttcggtct tggtttttag tttattttga tttcctgcca gcaaaatacc
      361 ttcctgacat aataatttag ccttcaatat atctttatcc tgcaagaaac ttgcatactg
      421 ataaaaaaca ggtaagtaac ttctgtttac agataaaacc tgctcaaaat attttaccga
      481 ttgtttctca tttcccaact taacatgttc aagtgcaagg gcatataaca caaataattc
      541 ctgtggatta tcggcataca tagcctcaag ttgctgaagt ctgtctgttg acatttcatt
      601 cacatttatt tcaatgaagt acaataatac ctatttttgc attccgaaaa aagtagttaa
      661 tcattttaaa tatgaaaatt ttagtttgta tcagtcacgt acccgacacg acaacaaaaa
      721 taagttttac tgccgataat aaatctattg ctacatcagg catacaatac attatcaatc
      781 cttatgatga aattgcactt acccgtgcat tggaacttac tgagaaatca ggcggaactg
      841 ttactgtaat taatgttggt gatccaagta ctgatgctac cttgcgcaaa gcattagcta
      901 ttggtgcaac agatgcagtg cgtattgatg ccaatccggc caatgcttct tttgttgcaa
      961 cacaaattgc agactatgca tcgaagaata gttttgactt aatccttacc ggtcgtgaat
     1021 ctattgatta caatggttca caggtagcag ctatggttgg tgaactatta aacatccctt
     1081 ctgtaagtat tgtaaaatcg cttgaagtag ctgataaaac tgtcaccctt gagcgcgaaa
     1141 ttgaaggagg gaaagaagta atttcatgcc ctactccact tgttgcaagt gcaacagaag
     1201 gaatggctga accacgcatt cccaacatga gaggtattat gagtgctcgt acaaagcctt
     1261 tgcaagttgt ggcagctata gctacagaaa catatcagca attggcaggt tatgacaaac
     1321 ccgcaccacg tacacaggta aaattaattg atgcatcaaa tgtaaaagca cttgttgatg
     1381 cattacacac agaagctaaa gttatttaat aataaaaatt tgagaattaa gatatgaatg
     1441 ttcttgtata tatagaaact gcagaaggaa aattaaaaaa atcggcaaca gaagttctga
     1501 gctacggtaa agtaattgct aaaaattcag gtggcacatt aactgctctt gctattggtg
     1561 atttaacaac tgatgagcaa ggcaaagcag gccgctttgg tgctgacaaa gttttacatg
     1621 ttactaatga taaattaaag cagtttataa atcaggcata cgcttcagta gtagcagaag
     1681 cagcaaaaca gaacaatgca tctgttgtaa taatttcaaa cacatttaat ggcaaggcca
     1741 tggcgccacg tgttgccgtt aaacttaaag caggtatggt tgatggtgca gttggtttgc
     1801 ctgatttaag taatggtttt aaagtgcgga catctgcttt ttcaggcaaa gcattcgctg
     1861 acattgagtt gacatctgcc ataaaagtaa ttactgttgc accaaacagt tttaaagcag
     1921 aagaaaatca atcagcagga acagtagaaa catttagtgc ctctgttgct gacagtgatt
     1981 tcactgcaat ggtaaaagag atagtaaaag cttctgacaa aatttcttta cctgaggccg
     2041 agatagtggt ttctgcaggt cgtggtttaa aaggacctga aaactgggga atgatagagg
     2101 aacttgcaac attattaggt gctgcaacag catgttcaaa gcctgttagt gatgcacact
     2161 ggcgtcctca tagcgaacac gttggtcaga caggattggc catcagccct aatttataca
     2221 ttgccatagg catatccggt gcaatacaac atcttgccgg tgtaagttca tcaaaaacca
     2281 ttgttgttat caataaagat ccggaagcac cattttttaa agctgctgat tatggtattg
     2341 ttggtgatgc ttttgaagta gtaccaaagc ttattgacga ggttaaaaaa tttaaagcag
     2401 ctaatggctg atcatattaa aagtttgttt acttttaaac cttgatgaaa aaaataagac
     2461 tcgatatttt cggtttgtct tatagtcagg cacaaagtgg cgcatacgcc ttggtattga
     2521 atgagcaagg cggcacacgc aggctaccta taataattgg aggctatgag gctcaggcta
     2581 tagctgttga gttggaaaat atgacaccca gccgcccttt gacacatgac ctttttaaaa
     2641 acttctctat ctcttttggt attcatattg atgaggtaac aatatataac cttaaagaag
     2701 gtgtatttta ttcaaagatt gtttgcaaca atggcgaaaa acaacttgaa atagatgccc
     2761 gcaccagtga tgccattgca ttggcagttc ggttcaattg tcctatttat acttttgatt
     2821 ttatacttgc acaagccggt gttgaaatgg atgaggactt aggtacttcg gcaacaagta
     2881 gcgaaatgcc ccaaatagaa aagcctgctc ccacacccag aaaacggact aaatcaattg
     2941 gcagtaacga tttctctcat ctttctacag aagaattgaa cgaagcattg attaatgcca
     3001 ttgacaatga agattatgaa gctgcttcta aaatcagaga tgagttaaca agaagaaaaa
     3061 agtagtttta taaagaataa actttctaac gcaagagcgt ttttctactt gtttttccta
     3121 ccaaacacag aaatattaac atacctccct gggtttgctt ttaagtctat cagaagttta
     3181 tcaagattag cagatgcatt gttgaggtta ttgtaaagac tgtcgtcatt gaccaactta
     3241 cccaaactgc cttgcgaatt atcaatctta cctaacacac tatttagttc tgcaattgtt
     3301 ttattgagtt tttcaatagt aaggttcagt tgcagtctgc ttaatgaatc actcagtgag
     3361 ttaacgtttt tcagaatacc gttgatgtcc gtttgcttgt tattgagatt accggccaca
     3421 ccatcaatat ttgaaagagc actgtttagt ttactgtttg aattgttcac cattttatcc
     3481 aggccttcag tagtactctc aagattggca aaaatattat tcaagctgtt gcgtgatctt
     3541 tcgttgatta gctgtgttaa tgcatacgat aatgaatcaa gtgatgtaac caacccttct
     3601 gctttatttt ttaatggctt aatctgatct gttaatcctg actgaacatt acccacaagt
     3661 gtatcaccat ttttagcagg tgttttatct tcactcattt caagttctat ggcttttgag
     3721 cctaacaaat cacttgaaac aattgctgca tttgacgatt tagggataaa cacattatca
     3781 tctactctaa gtgttacaat aagacgcccg cttttatctt cggcataaac aattttatca
     3841 acctgaccga ttttaaatcc gttcattata accggatttg attttacaat tccatttaca
     3901 tcgtcataaa caacataata tttatttgac gtagaaaaca aatctgttcc ctttaaaaag
     3961 ttcatacccc agaaaaaacc ggctatggta agtgtaaatg ctatgcctac ttttacttcg
     4021 tttgatattt tcacgtgatc tgaatttatt ttttaattgc ttctgcctct ttatttgaaa
     4081 ttcgtttgcc tttataatat ggagtgacaa atgcatcttt ataacctttt gcccgcacct
     4141 gtgactgtgc tgccaatgct ttggcatgtg ttgtataaac gccttgacac actctgatca
     4201 ttccattctc tgctttaaaa gtcttgatgt ctgtcaggtc ttcaaatcgc gttaattctt
     4261 cttttgatgc atcaggcaat gctgcaagct gaacggtata aaaaaagtca ggtaatttct
     4321 ctgtattaac ggcttttttc ttctcctctg ctggaaggtc tatttcatca ggagcaacgg
     4381 gcttaaccgg agattctgtt ttgttatttt ctttcggctg cgtctcttta tttttaccgg
     4441 cattgtctgc attatttttg gcagcgtctt tttttacgtc ctgatcttct ttattgctgt
     4501 ttggaatagt agaattactt tcgttctctt ccaccttttt ttgtggctga gtagtttcct
     4561 tagccggttg gttgtcgcta ttttttactt caaccacatt tttattcaag tcattctctg
     4621 cacctccctg aggaatttta gctcccggag tttccatatc taatttatac tccataaaag
     4681 ccctgaatat acttgtagca atagcatttt ggcctttctc tgttagtaaa tatttttctt
     4741 ctgttctgtt ggttaaaaat ccggtttcta tcagcactga tggcatagca gttttataga
     4801 gcaccaaaaa acctgcctgc ttaacaccac ggttataacg tcctgcatat acttcaaact
     4861 gctgttggac ggcagaggca aacttgaggc tttgtgtcag gtaggcattc tgatataaag
     4921 taaaaacaat attggcctca ggcgaattgg ggtcaaagcc atcatacttt tgtttatagt
     4981 ctttttcaaa cagcacagcg gcattttctc gcttggccac attcagattg tcttctgatt
     5041 tatgcaagcc cataacatag gtttctgcac caaaagctgc agggctgccg gagttgcaat
     5101 gaatgcaaat gaaaagctct gcatggttgc ggttggcaat agcagcacgc tcatgcaatt
     5161 caacaaactc atctgttttt ctggtgtaaa taacctttac gtcaggatat ttttgggtaa
     5221 tcatttcacc caatttaagt gccacttcaa gtgcaatctg tttttctttg gaagatgcgc
     5281 ccaaacagcc tgaatcatgt ccgccatgac cggggtcaat aacaatggtc ttaatgcgat
     5341 atggttcctt atatgttcca ttagacgctg acaaacagat taaaaacaac agtgaaagtg
     5401 taaaaaagac ccgtattttt atcttcttat aaataaacaa catgattctg cgtttctttg
     5461 gatggtgtta aattctgttc aattaccttt gcggctccaa aaatatcgtt tttaactcca
     5521 tctccttttt ttgtaaatga ccgtttttaa aaacttatta acgattgcaa tattgtggtg
     5581 cctggcggtc attgtatttt caactgaaaa tattcatgca caaacgcttc caaaagactc
     5641 attatccaca gatacccttg ttaaagacac ccttaaaacc ggtgtcagta atgacataaa
     5701 atcaaaagtg cattatgtag ctgatgatac cattgaattt gatgttgaac atgaaagggt
     5761 ttttatgtat gaaaaggcac aggttaatta tgaaaatatt gaactgaaag ctgcctatat
     5821 tgaagttgac tggaatacta aaaatgtttt ttcggcagga cttcgcgatt caacaggaaa
     5881 actctatgga atgcctgttt ttaaagaagg cgatgatgaa tttaaagccg atacgatgcg
     5941 ttataatttc ggcacaaaga aaggcaaggt ttatcatgtt gtaacacagc aaggcgatgg
     6001 ctttattcat ggtgctatag ttaaaaaggt ggaagaaagc agttatatac gctatggaaa
     6061 atacactacc tgtagtttag acaccccaca ctattacatt gcagcgaata aactcaaagt
     6121 cattcctaac aataaaataa ttacagggcc cgcttatctt attattggcg atgttcctgc
     6181 accggcagcc attccgttcg gattatttcc caatacaaaa ggtcgtagtt caggaatatt
     6241 attcccggca tacggtgaaa gtaatgcact tggatttttc tttaaaaatg gaggatatta
     6301 ttttggaatc agcgatcatg ttgacctgca acttacagca gacatctaca cgttaggtag
     6361 ttgggcagta aaagcaactt cgcgatacaa taaccgttat cattataatg gaaatttatc
     6421 tttcctctac tctatcataa aagtaagtga gaaagattta cccgactata gccgtagcaa
     6481 agagtttttc ataaactgga gtcacctgca agacccaaaa gcaagaccca acggaatttt
     6541 ctccgcttct gtccgtgccg gctctaacga ttattacaca agaaatattt caaacgcatc
     6601 aaactacctg acaaatactt tcaactcatc aatttcttat tcgcgttctt ttttcaacaa
     6661 acaactcaac ttctcatctg ccatcaccca ttcgcaaaac acgcaaacca gaattgtgta
     6721 tttaactgca ccaagcataa accttagcat gaatacattg tatcctttcc gtaagaaaga
     6781 gcaagagggc acactcgctt ggtatgaaaa aatcagtgtt ggctacagca gtactttaca
     6841 aaatcaatta cagaatcccg atagtttatt ctttaaaaac actacactgc agcagatgaa
     6901 aaacggaatg caacataaca ttccaatcag tgcatcattt aaagtgatga aatattttaa
     6961 tcttactccg gctatcaatt ttaacgaacg ttggtatatt gaaacataca aacaacgatt
     7021 taatgccgac agtaacagaa ttattaccga tactgttaaa ggattcaacg ctgcacatga
     7081 atacaacggc tcgttaagtc ttaacacaag aatttatggt atgaagcaat tcaagaaagg
     7141 aaaaatagct gcaataagac atgtgctttc tccaacatta agttatatgt acagaccgga
     7201 ttttggtgag aataaatatg gttattacaa aaccgtacaa actgacagcc ttggcaacag
     7261 cagacgatat tcaatctacg aaaacacact atatggtggt ccttcactag gaaagtcaaa
     7321 tgtgcttagt tttagtctag acaataatct ggaaatgaaa accagacaat acaccgatac
     7381 ggcagtgaac attaaaaaaa taaaaatctt agaaagtttt ggcctgtcag catcctacaa
     7441 cctgtcagca gattcattta atctttcgcc tattggcatg agtgcccgca caacactttt
     7501 tgatatggtc agcttaacag catcaggaac atttgaccct tatctgatga acgatcaggg
     7561 aatacgaatt gcaaaatatt cacttgcaga gaatggtaaa attgctcgtc ttacctcagc
     7621 aagcggaagt gttggcttta atctgaatcg tcagaaagga aataaaaaaa gcttaaaagg
     7681 aactgttgaa caggtaaaag aaattaatga tcatcctgac gattatgttg acttttctgt
     7741 tccctataat ttatcggtca attacagtat tgcctattca cgacctacaa atgaaaaagg
     7801 aaatctatcg cagatagttg gttttaatgg tgacatttca ctgactgaac attggaagat
     7861 aacattcaac tctggttatg attttcaaac caaaaagcag tcctatacat ccttgggttt
     7921 ttatcgcgat ttacattgct gggaaatgcg tcttaactgg gttccttttg gatatcaggc
     7981 aaattattat tttcaaatca atgttaaatc aagtgtactt caggatttga agttgacgaa
     8041 gaaaaacgac cgttacgata atttctaaat ttactcaaca ctgaatttgt gtccttcaat
     8101 ggcaagttct gtatttttaa ataccgttct ggcttcatct aataatggtt ttaagtcgcg
     8161 atagcgtgca gaaaaatggc ctacaataag tttgtcaaca cctgcttttt ttgcaatagt
     8221 tgcagcctgc agacttgttg aatgaaacgt tgtttttgcc cgttctgcca agtcattcat
     8281 aaatgtagct tcatgataaa gcaggtttac tccttttatt tcatcaataa ttgattcatc
     8341 ataacaagtg tcgctgcaaa aagcataaga gcgtggtgct gttgacgaaa tggtaagctc
     8401 tgcattggga attattttcc cttcatcgtc ctcataatca aaaccgtctt ttagtcggcc
     8461 atagaatgaa aatggaatat tatgttgctg tagctttgct ataattaact ttcttggttt
     8521 tggcttttca gaaaaatgat atcccgtaca aggaatacgg tgattcagaa taattgattt
     8581 gataagcata tcatcatcct catatattat tgaaggtgca taatgctgta caggatgaaa
     8641 aattaactga tatcgcaatc gtgtttgcga caatcgcaat tgaagttcaa taacatccat
     8701 taactcaggc tgaccgtaga tgtgaatttc ggtagtacgc ccctgcagat gcatggttga
     8761 aagcaaccct acaagaccta aataatggtc gccatgcagg tgtgaaataa aaatatgcga
     8821 aagtttattg agcttaaact tatatttcag aaactgccct tgtgtgcctt ctccacaatc
     8881 aatcaaaaaa aaccgctctc ggatattaag aacctgagcg gtgggatgac cttgatgaat
     8941 tggcgttgcc gagctactgc ctaagatcgt tacttcaaaa ttcattactt ggaaataacc
     9001 atccttcttg tgatgttagc tgtgcctgaa ttgatagaat acatataaat tcctgcagaa
     9061 agattttcgg cattgatagt ataagtattt acgcctcttt ttgcagaaat tgtttcactg
     9121 aaaatatttt tacctagaat gttataaatc ttaaaatcaa ttttggtgtt aaccggtgaa
     9181 ttaaaatata taactgattt gtttttaaaa ggattcggca ggttttgcga catgcttagc
     9241 ttatctttag gcagcaactc tataactccc gaattggtgt caatggtaat ggtataatag
     9301 gttaccgaca tgggttggcc gtaagatata cctccaaggt tagtaatttt tcctctggca
     9361 atgatatcaa ccgtaattgg atatacacct gcagttactg gcgtacctga aagtagaaga
     9421 caaccgttgc tacttgctaa aaactgacaa gttcccgggt tgcatgcata ggtcagtccg
     9481 ggaggtaacc ctgaaacgcc taaaatggta attgagtcca actcaaaatt tgcattgaag
     9541 ggtacaccgc caatattgat agttgccgtt gtatcaaaaa atgttctaac ctgaataacc
     9601 tgattgtagg ctacgcccac catgccattg ctcaagcctg tagcactgtc gggataaaca
     9661 ccgggatcaa ttattgttgg gtcaggagta cactgcgaaa ctgcaatgcc tgacatagag
     9721 ataaacatca atgagaagag tagctttttt atcatatttc tgaattttat tttcaactta
     9781 cagcagcggc tttattgatt tgagccaatg cttcatcaat ttttggaaca attgttaata
     9841 ctgaatcaag ttgagaaatg ttgattagtt tttttaccgg atcgctgatt tcggttacaa
     9901 tgaaattacc attggcattg cggcaaattc tgttgcctac taaaatagca ctcagtcctg
     9961 aagagtcaca atattgtgtg tcggaaaggt cgaggacaat atgaatgtag ccctgactgt
    10021 tgagtatcaa caattctgac tttagttcag cagaaataag tgtattaagc ttttgttcgt
    10081 gaagctttag cactgcgtat ttttctttct tgtctaaggt ataatgcata atagttggtt
    10141 gtaaaattgt tgcaaacaaa tataaaaaag atattctaat aaaaaacaaa tcggcttaac
    10201 gacttataaa atcaacctac aggctgacca gctaataaat ataaaactgc catgcgcaca
    10261 gcgacaccat tttcgacctg atctaatatt atagattgat ctgaatcagc tacttcagta
    10321 gtgatttcga ccccacggtt gattggcccg ggatgcatga tgacaatttt tttctgtaat
    10381 tttttcagta tgtcgagcgt gattccatat tgcataatat actcccttaa ggatgggaaa
    10441 tatttaatat cctgcctttc aagttgaata cgcaatacat ttgctacatc gcaccagttc
    10501 aatgcattaa taaggttggt ttcaaccttc acacctaact gctcaatata tttcggaatc
    10561 aacgtagcag ggccgcagac catcacttct gcacccgatt tttgcagaca atatatattc
    10621 gacaacgcta ctctggaatg taaaatatca cctacgataa ctactttttt accttttagt
    10681 gttcctaatt tttggcggat agaaaatgca tcgagcaatg cttgtgtagg gtgttcatgc
    10741 gtaccatcac ctgcattgac aataactgaa tttacttttt gcgataaaaa caaagctgca
    10801 ccgggtttag ggtgacgcat caccaccata tcaaccttca tggcaagaat attatttacc
    10861 gtatcaatca atgtttcgcc tttgctgact gacgatgatg atgcagtaaa attgagcaca
    10921 tcggcagaga gccttttctc tgccagctcg aatgacagac gtgtacgtgt agagttttca
    10981 aaaaataaat tcactacggt aacatcacgc atggaaggca ctttcttaat gggccggttg
    11041 atgacttctt taaaagtttg tgccgtatca agaataaggt tgatatcttc aggattcaga
    11101 tttttaattc caataagatg tttaacgctt agctttccca ttcgtttttg atttgatttc
    11161 agaaactaac cacacgccat ctttgctgcc actctccttc cactctacct gcactttatc
    11221 gttaacagtt gtatcaattg cctgtcccgt ataatcaggt tctattggaa attgtctgct
    11281 gaatcttctg tcaattaaaa ccaatagctc aaccttggca gggcggccaa aggccatgag
    11341 tgcatccatt gctgctctta tggttcgtcc ggtaaacaat acatcatcaa ccaaaaccac
    11401 cttcttacct tcaacaataa aatctatctc tgttcgtgac ggcactaaca gcttttcatt
    11461 tctgcgataa tcgtctctga aaaatgtaat atccaaagca ccatgaagaa tattactctc
    11521 cggcaaaaac tctgaaagtc ttttacaaat gcgtcttgac aaagggacac cttttgactg
    11581 aagccctatg atgcaggtat ctttaaaatt tccgtggttt tctattaact gatgacaaag
    11641 gcgctctatg gtaatatcta actgtttgcc cgcaagaatt aaatgtgtca tcgtgtggca
    11701 aaaatagcat ttgatcttaa ttgtaaaaaa tcaaattaca ttgccatcta aaaattcaca
    11761 ataaaaattc cggaaggcgt aaaatacttt aaagctaacc cgctaattaa acggttgcct
    11821 tttgcattct taattgtgga ttcaaatgtt ctatggcatc tgccaagaaa caaggaataa
    11881 tggcatggtg cgtcagctca attttattcg gatcgctgag cgattcaatc atatcataat
    11941 atttttcact gccgtaatgt tcaataattt tttctttttt atcagcgcga tcaaaataac
    12001 gatacatacc ctctgcatca gcctcgggat ggaactgcac acccaatatt tcatcactga
    12061 atcgcaatcc catgatagca cgctcatagg gaacatgagg tcttattttt tcaagacaaa
    12121 gaagtttgcc gccactgcgc acaattttat caatgtcagg agttgtcacc tgccattctc
    12181 tggagtcaac agcaaaaaac ggttcaggaa gacggccaat aattctgtca ttattgccgt
    12241 cttcagtttt atgtatcgga aaaattccaa atgatggcga atgtctttca ctcactatgc
    12301 caaagcctaa gtgatgtgcc atcatctgaa atgaatgaca aatcagaaaa gcatacttct
    12361 ttttattttc agcaatggca ttgtgctcta taagtttatc ccaaagtgcg taaaaaggtt
    12421 cttcccaagc ttcgcctgca ggcaatgggc agccgggtcc acctgaagaa atataaatgt
    12481 cgtagccaag gtcaggcact tcattatttt gtctgacatc aaaaaagcta cattcaataa
    12541 atttactgaa tcggtcaaga atggtgcgga tgctgcgcat gccttcattg ggttcaccat
    12601 tatataagtc gagcactgca acgcgcacag gaataaaatt tgtcattact ttttttcctt
    12661 ttttacggga atatcaatat ttgtttcact aattatgtaa tctaccactt ttttcaggtc
    12721 accggttttc tcaaaaactg ccaactgtct gtcggcaccc gtaccttttt tcatcatatt
    12781 aaaaacgtgg tttacctctt tgcgtgagcc taaatcaccc acgacatcat caataaactc
    12841 tgtaatttct tcaatcagaa cttttgttgc cacctctttc tctttaccaa aatcaatcag
    12901 tttgtcatca ataccataac gtgcagcgcg ccatttattt tcattaatta atgctctgga
    12961 gtaggttctg aaactcaggt tgaactgcat gagcaaatac aacttggcaa tgatgccttg
    13021 catgatagcc gccagcgtaa tagtttcttc aatacgcata ggaatatcgc aaattctgaa
    13081 ttcaatagta ttgaaaaaag gatgcaatct gatatcccac cacactttct ttgcattgtc
    13141 catgcagttg gtctttacca gtagttgaat gtaggcatca tattcggcag cacttgaaaa
    13201 ctcatcagga atacccgttc tcggaaattt atcaaacacc ttagttctaa aagatttaaa
    13261 acctgtattt ctaccttccc aaaaaggaga attggttgag agcgcaaaaa tgtgaggcaa
    13321 aaaataacgt gcctgattca tcagtctaat acctatttct ctatcatcaa tgccaacatg
    13381 cacatgcaat ccaaaaataa ggttgcttct tgcagcatcc tgcatctcac gtataatttc
    13441 ttcatagcgt gggtggtcgg taatgagctg atcttgccag cgcgaaaacg gatgcgtgcc
    13501 agcagcagca atcaccaatc cttgtttatg cgccagctta gcaaccatct gtcgcagata
    13561 ggtaacttct tttcttgctt ccgaaacgtc tttgcagatg ttggtaccca cttccactac
    13621 actctgatgc atttcagctt ttacctgctc tttcaaaatt accttaccgc cttcaacaat
    13681 ctgatgcatg tgcgaacgca actctcttgt tttaggatct attacctgaa actcttcttc
    13741 cacaccgata gtaaactgtg gtaatctgaa ttttttattg atatatgcca tactctaatc
    13801 agcacttttt atttcactgt gtatgattcc atcagtgcgt ccatattctg cttgttcaca
    13861 atctgccctt gtgcagcatg tttaacaaaa gcaccccatg tcaaattatc ttgcccttcg
    13921 cggtgtgcaa gtgccttctc aatacacata tcggcaacgg cttcaataac ccaattaaaa
    13981 ttctcttcac ctacactatg gcggtcggca tcgggtgcgg gattgcagaa gtctatggca
    14041 tagggcacac catcacgaat ggcaaactca accgtattga tgtcataacc caaagcattg
    14101 caaagtttaa aagtatattc ttcaacaaca tcaagaatac gttgctcagg tgcgggtccg
    14161 tcaaccacat aacgcaggtg gtgcgggttg cgtggctcat aacgcataat ctttatgcgc
    14221 ttgccgccta tacaataaca gcggaagtac tgatcaaaca caatttcttc ctgcagcagc
    14281 atcaccaact gacctgtctc ctgatgttta ttataaaaat cggtagcgtc attgcattta
    14341 tacacatttt tccatccgcc acctgcaaaa ggtttcatat aggccggaaa acctatgtac
    14401 ttaaaaatat tatcccaatc caaaggcatc ttcaggtttc tgaaagaatt ttcatttgta
    14461 tcgggtggat gaacattact tggcagcaga accgtctttg gcaccggcac accaattttt
    14521 gtagcaagac agttattaaa aaatttctca tcggcactgt tccaaaacgg gttgttcagc
    14581 actgctgttc cgcagatggc agcattcttc aacataccac gataaaaagg cacatcgtgc
    14641 gatattctgt caacaatcac agcatagtcc ataggcattc cctgcaccac cttatccacc
    14701 tgcacggact cggccataat gccactaata tttttagagt taatccgctc tacaactgcc
    14761 ggtggaaaac tgttctcctg tccgaacaaa acgcctattt tcttcattgt ataatatata
    14821 tgtgttatcg cacaaaaata acaaacagat gcaaatgggc aaagagttgt aaagttggtg
    14881 tgtattgtcg tgaaataggt ttacacaaac aactgaaaat gggtatagtc catttgatag
    14941 ttttttaatc attgaataaa agtatgaaat tgaaatttat atttttattt ttgaaaaata
    15001 atgcgaaaag caaaatacac ttatccgcta aatttgagcg taaaagcgaa taaaattatg
    15061 ttttgtggcg catataagta agttagcggt caggcagaaa aacaaacatc cttataaaaa
    15121 tagatgacag aaaaagttcg taaattaggg cattaaaaaa gtaaagaaat gacaaaagta
    15181 tttatagctg gagcaacagg ttgggcaggt tctgaactaa gtaaaggggt gtttcaaaac
    15241 aaacagatgt aattagtagg agcactttca agaaaacata aggacaaaaa tttggcagat
    15301 atactaaatc ttggcaagtt aaatattcct atttttgaaa gcattgagac tgcattagaa
    15361 aaagttgact ttgacgtttt agttgattat acacgtcctg aaattggaaa aaagaatatt
    15421 atatcgacaa ttaaaaaagg caaaagcgtg gtagttggta catcaggctt gactaatgat
    15481 gattatgtag aaattgaaaa attagtattg gaaaacaaca catctgttct tgctgttgga
    15541 aactttgcaa taactgtagt attactccaa aaattttcgg aaatggcggc taaatatatt
    15601 ccgaattttg aaattataga ttatgcttac gaagataaaa tcgacagccc aagcggaacg
    15661 gcaagagaat tggctcatag actttctcaa attcaaaaac cagttgttca cgtttctaat
    15721 aacgaattag ttggagagaa agaaagtcgt ggtgcaaact tagacggtgt acaagtacat
    15781 tccgtgaggc ttccaggaca cgttatttcc atagaaagca tctttggact gaaaggcgag
    15841 aaattaacta ttcggcatga ttctggagca agtgctgaac cttatgttaa aggagccttg
    15901 ctatccattg aaaaagttgg aacatttaaa ggtttaaagc gtggattaga ttcggtaatg
    15961 gagttttaat cctgaaaaat atgaaacaaa taaattgcac gaaccgctaa tcgagtaggc
    16021 ggctccgcca gatactgacg gagtacacct ctctcaccac taagcttacg cgagctacgc
//
LOCUS       DAOQNP010000072        15948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1301_length_15948_cov_9.079280, whole genome shotgun sequence.
ACCESSION   DAOQNP010000072 DAOQNP010000000
VERSION     DAOQNP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1301_length_15948_cov_9.079280"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(527..600)
                     /locus_tag="PKY09_09830"
     tRNA            complement(527..600)
                     /locus_tag="PKY09_09830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(564..566),aa:Ile,seq:cat)
     gene            681..1307
                     /locus_tag="PKY09_09835"
     CDS             681..1307
                     /locus_tag="PKY09_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNB13198.1"
                     /translation="MIKNIIFDFGGVLLPINTEKTAIEFAKMGLDNFEELYSINKQAK
                     FFDDFEKGLIPASAFRSEIRKHYKRPLSDEEIDYAWNALLGELNPERFNFLSELGDKY
                     NIYLLSNTNIIHHAAFIKSLNQTFGKDRFEKLFEKTYYSFQMGTRKPDKRIFTDVIKD
                     SGVVPAETVFIDDNLNNALGAQKVGMNAIHHDHAMEIEKELMAMLSAF"
     gene            complement(1304..1708)
                     /gene="mce"
                     /locus_tag="PKY09_09840"
     CDS             complement(1304..1708)
                     /gene="mce"
                     /locus_tag="PKY09_09840"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNB13199.1"
                     /translation="MKNLEHIGIAVKSLQISDPLFEALIGCPPYKRESVVSENVITSF
                     FQSGETKVELLEATSPESAIAKFIEKRGEGIHHIAFAVDDIKAEMQRLQQSGFTLLNS
                     EPKKGADNKLVCFLHPKSTNGVLVELCQEIKS"
     gene            complement(1695..2393)
                     /locus_tag="PKY09_09845"
     CDS             complement(1695..2393)
                     /locus_tag="PKY09_09845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNB13200.1"
                     /translation="MHPEARKIFKSFTVPLLLSLLMIGVKVFEVLSHTDLGVYGVLPR
                     QISGLKGILFIPFLHADWEHLFSNVIPLLVTGSMLFYFYREMAVKVILLIYILSGFWL
                     WLGGRENYHIGASNIVYGITAFLFLSGFIRKHTGLIAVSLLMVFLYGSLVWGLFPVVP
                     KISWEGHLFGALSGILCAIVFRKEGQQQEVYEWTDDDEPFDDHENDDNAPQSSLKIVY
                     SYKKSDEKNGTEES"
     gene            complement(2549..2995)
                     /locus_tag="PKY09_09850"
     CDS             complement(2549..2995)
                     /locus_tag="PKY09_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB13201.1"
                     /translation="MVTIRKGTPADVAPAFRLIKELAEYEKAPEQVINTVEQMLKDGF
                     GEKPIFDLLVAETENNIAGIAIYFIKYSTWKGKCLYLDDIVVQESLRGKGIGQKLFDA
                     VAAEAKLLNCQQLQWQVLNWNEPAINFYKKYNTVFDDDWINCKIIL"
     gene            complement(3000..4589)
                     /locus_tag="PKY09_09855"
     CDS             complement(3000..4589)
                     /locus_tag="PKY09_09855"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB13202.1"
                     /translation="MLEVKNLKVVFHQNCNPVVAVNEFSFSITSGETLGLVGESGSGK
                     TVSSLALLGLLNNKNVSISGNAFLNLQQPVDLLQLTEKQLTAIRGRHIAMIMQEPMQA
                     FNPLFTCGHQVAETIRKHTALNKNDAIEKVLQLFEKVKLPNPLQISKRYPHQLSGGQL
                     QRVAIAMAISCNPSLLIADEPTTALDASVQKDILVLLQQLQSENNMSMLFISHDLEAV
                     KLIAHRTLVVHAGQIVEQGKTETLFAHPIQDYTKELLQSRLSTFKKNSVIGEKIILSV
                     ENLNKSYNQRQGLLRSPKNITNAVDNISFTIKQGETLGIAGESGSGKTTLAKLLLRLI
                     ETDSGKIKLNEEDLTHYPIRTKQEWRKKIQAVFQNPHGSLDPEMTVGRQLTEVMQLHD
                     SPQNATIVKNKIIDWFEKVGLTEQHFNRFPHELSSGQKQRLCIARALIPAPDLLICDE
                     AVSALDVSVQAKILELINSLQQQFHFGCIFISHDLKVIRQMCDNVLVMKDGKADCYLE
                     TEKLFKQPPTTYSEILLKSIL"
     gene            complement(4631..8203)
                     /locus_tag="PKY09_09860"
     CDS             complement(4631..8203)
                     /locus_tag="PKY09_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNB13203.1"
                     /translation="MWSILRFELSYRFRRPATYIYLAILFLISFLFAATDAITIGGAV
                     GNIHKNAPYTIYQVMLILTLFGTMIVSAVMGVPVYRDYEHRFHEIMFTTPISKAQYLG
                     GRFLGSYFTSLFIFLGIPFGILTGVMMPWIDQEMIGPFMLNAYVLPFLYIIIPNVFIL
                     GVMFFATGSLFRNQLAIYLQGMVFFIFYLVLTSATGDIENNPKFALLDVFGITPSFYI
                     TRYWTPFEKNLQTIPLESYLLWNRLLWMGIALIGAFFTFRLFQFSKTSPFVLRSRKNQ
                     EKINVESLSHINLPETSKDYSFSTRLRQWLQMVRFDFFGTIRSVLFIAIWICGVILIL
                     GQMPQIGKMFGTTIYPVTYAIIDDLAGNFTLFTLIIITFYSGELVWKERERGIHLITD
                     SLPVSRYALITAKFFAMCLIVAMLQLTVMFMGILIQTLKGFYRYDIDVYLKTLFVSQY
                     PSLILLVLLAFFIHSMVNNKFIGHTLMIAYFVGRMAAAAMGITHKMIWYGNIPSAPYS
                     DMNGYGHFLYPKYMFLLYWGSLGLVLFILGVMLHRHGSEIGIKARWQIFKQQWQYSRG
                     RKIVWSLLLLFIISGSFIYYNTNILHKFKSDKTVRHEQAEYEKRFHKYLDFNQPRITA
                     VNVNIDLVPEKRYAAIKGYYIIQNKSALPIDTFLITWAHENIKINRFGFDILADEVEN
                     YQKQFVIYKLAHALKPGDSTKVYFDIEYINNGFANTEEESGLVNNGTFINNNYLPAFG
                     YSESFELTDNSDRKKEDLPRKNHTMRLLSDSASRNNTYLANDADWIRYECIISTSPDQ
                     IAVSPGYLQKEWTQNGRRYFHYKMDAPMVNFYSFLSARYEVFRDSFQGINIEIFYHKT
                     HSWNIEKMNHAIKKTLAYCQKNFSPYQFRQVRILEFPRYETFAQSFPNTIPFSEAIGF
                     IYDVSEDTDIDNAFYVTSHEVAHQWWGHQVCGANSQGSTLMVESMAQYTALMVMEKEF
                     GKDAMEKFLKYELDGYLSGRSTERINEQAIARNDGQGYIHYQKGSLVMYAMKDYLGED
                     TLNAALHRFIKTYGFQSPPYVQAPQFVKYIADATPDSLKQVVEDMFNRITLFDLKCVE
                     SKYKKIGNKYEVTLDLETGKVYADSMGNESGVKPNDWIDVGIMKYDGNGKHTYQYLQK
                     QLFTGGRKTIVVIVDEKPSKAGIDPLHKLIDRNSADNIKEVKSL"
     gene            complement(8207..9085)
                     /locus_tag="PKY09_09865"
     CDS             complement(8207..9085)
                     /locus_tag="PKY09_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB13204.1"
                     /translation="MKLTIKGLTKTYPNGVQALKNINLEIKNGLFGLLGPNGAGKSSL
                     MRTIATLQNADSGNIFLDDLNVLEDKEEVKKILGYLPQEFGVYPNISAQELLNHLALL
                     KGVHLKKERDEVVKSLLLQTNLYEVRNKNLGGFSGGMKQRFGIAQALIGNPKIIIADE
                     PTAGLDPTERNRFLNLLSEISEQVIVILSTHIVEDVKELCSQMAIINKGEVMFSGHPA
                     EAIRQVQGKIWTCQVDKPTARELMTKYNVISQKFNTGLIELHVWSEEPLNHHFVIAQP
                     VLEDVYFSVLNGNLKP"
     gene            complement(9152..9505)
                     /locus_tag="PKY09_09870"
     CDS             complement(9152..9505)
                     /locus_tag="PKY09_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13205.1"
                     /translation="MKRFKILTVILVAAILPAFAQNSQPPVVVPAYISQGEVMPYQQL
                     PEVIITPNATEQKAVQLPVVTITANKINQQIYPAVKYGNNYIASITISPIDVVSHQQK
                     RTFASLFSFLRYFSR"
     gene            9787..10131
                     /locus_tag="PKY09_09875"
     CDS             9787..10131
                     /locus_tag="PKY09_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNB13206.1"
                     /translation="MHHYLFSYGTLQLEQVQLENYGRKLKGSNDFLNGYRIEDLVITD
                     IEVIRKSGKHIHPVAVCSGNKNDVIKGMIFEITEEELQATDKYEVSDYVRVMEKFESG
                     KEAWIYIGKIKS"
     gene            complement(10136..11365)
                     /gene="serA"
                     /locus_tag="PKY09_09880"
     CDS             complement(10136..11365)
                     /gene="serA"
                     /locus_tag="PKY09_09880"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNB13207.1"
                     /translation="MSLTSFPKEKIKILLLEGVHPAAVAMFKDAGYTQIETTTGALSE
                     KELLQKISNIHILGIRSKTQLTAAVIAQADKLIATGCFCIGVNQVSMGDAIENGIAVF
                     NSPFSNTRSVAELVIANCIMLLRRIPEKSNAALAGKWLKDSAGCFEVRGKTLGIIGYG
                     HIGSQVSVLAEALGMQVIFYDVEPKLILGNAKACKSLNDLLKQSDIVTLHVPGTSATR
                     NMVNESFLRKMKKGSVLINLSRGEVMDEGAIAKAITEKHLAGLAADVFSDEPQHKGDI
                     FQSPLQHLNNVLLTPHIGGSTLEAQENIGTDVAGKIIRYLETGNSMGSVSVPALNLPL
                     QQSAHRILHIHRNVPGVLSEINTTMSKHKANILGQYLQTNNTIGYVAVDIDKKASSGL
                     ISDLKNSKETIKVRGVY"
     gene            complement(11509..13293)
                     /locus_tag="PKY09_09885"
     CDS             complement(11509..13293)
                     /locus_tag="PKY09_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13208.1"
                     /translation="MKKLILFLTIVSVQFSVRAQIPSYITDYSSFNALTLEGGQTEIE
                     FADINNDGHLDLLTVGDHGSPLINTSQHGISVFFGNGTGSGWTLYQNGDFGYGGIAVG
                     DVNNDGFLDVGYGIHHNYSSTDFGDQLLEVALGDGTGMNWTPWDDSLATAGETWGMFG
                     TDFGDFNNDGLLDIVSGSFGCCAGTHVYLNSGTGVWNHSFGYINGNTEHYVECGDINH
                     DGNLDFVSCHQNGAAYFGDGTGNFTNSHFNLPPIGPLGFYDISLKDIDGDGDDDFGFI
                     HNNSVPYVYKWDNVAQQWVNSSSGLTVSGLASCIKLADLNNDGFADAVVATTGSIVIF
                     LGNGGTVWNNVLTIPLTNMTFCQDIAIADADQNGYPEIAVWATFNNGPFSTINKLVYL
                     REVTPVTTLNINQTFPKGYECMPANAVRNITWNSSVIPGHQTSVKIEFSSTGLNGIWS
                     TVAAAAPNNGNYQWNVPSGIISNNSYIRTILTDSTAMATDTAYNLHPFQIGVCDPALS
                     VSALENNFDFDIAPNPFSNTFSISAKNKLAKIEIYNTLGNKVFSEDLKLMLKQSYEVK
                     PSLNSNGIYFIKLTTVTDEVKTMKIVRE"
     gene            13457..14485
                     /gene="fbp"
                     /locus_tag="PKY09_09890"
     CDS             13457..14485
                     /gene="fbp"
                     /locus_tag="PKY09_09890"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNB13209.1"
                     /translation="MYTPKRVITLGQFIIERQSDFPYAKGELSRLLRDIGIAAKMVNR
                     EVNKAGLVDILGDYGNTNVQGEEVKKLDVYANEQFISALSSGGETCAVASEENEEMVP
                     INSEISKNAKYVVAMDPLDGSSNIDVNVSIGTIFSIYRRTTLSGPSNLSDFMQKGTEQ
                     VAAGYVIYGSSTMMVYTTGKGVNGFTLDPSIGEFCLSHPDMKIPKQGFIYSVNEGNYA
                     HFPEGVKKFIKFCQEEDEKSKRPYSSRYIGSLVADFHRNLLKGGIYIYPATAKSPKGK
                     LRLLYECNPLAFIVEQAGGKASDGFNRILDIQPTSLHQRTPLFIGSPDMVSKAEELMM
                     RFSQVSTS"
     gene            14558..15538
                     /locus_tag="PKY09_09895"
     CDS             14558..15538
                     /locus_tag="PKY09_09895"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNB13210.1"
                     /translation="MREIQFREALREALSEEMRRDPNVFLMGEEVAQYNGAYKVSQGM
                     LDEFGAKRVIDTPIAELGFAGIGVGAAMNGLRPVIEFMTFNFSLVAIDQVINAAAKMK
                     SMSGGQYGVPIVFRGPTASAGMLSSQHSQAFESWYANCPGLKVIVPSNPKDAKGLLKA
                     AIRDDDPVIFMESEQMYGDKGEVPEGEYLMPIGVADIKRAGNDVTIVSFGKIIKTAYK
                     AAEELAKENIDVEIIDLRTVRPIDYATVIASVKKTNRMVIIEESWPLANIATEVAFKV
                     QREAFDYLDAPVLRVTGGDVPLPYAPTLIDAYLPNPERIIRAVKRVMYLE"
     gene            15746..>15948
                     /locus_tag="PKY09_09900"
     CDS             15746..>15948
                     /locus_tag="PKY09_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13211.1"
                     /translation="MDTIAIGDTLTVNNTSSYPPNTIFVWNNGEYCRTTNLTGIAGGE
                     VLINDSLCNDTVIGSSPINFIYE"
BASE COUNT         5018 a         2970 c         2884 g         5076 t
ORIGIN      
        1 aggcaatatt tactgtttgt gattgcgccc ttgtaaagaa tgtaaacatg cagagcaaaa
       61 gggctattgc agttgtttgg atttggtttt tcatattatt tggttttttg ggtgtgacta
      121 aagtacagta atgttttgga gtttgtaggg gttgaataaa aaataaaacg cgggctgatt
      181 ccagtgctcg tatcggaggg ggtctaatcc cttaccacga agcaaaagga acgcccgcgt
      241 tttacacagg cgtttccact tttactctcc tgtggtaatt gaaaaattag acctttccga
      301 tacaggaatt ttaaagcgaa acgctaatat ttttttacaa ttttattcta ctttatttat
      361 tcattttaat gattcatctt tcttttgtgc ggcattcatt ttccgctttg taaaagtggg
      421 aaatgatcct gaaacaaaca aattattttt aaattatttg taaataacac attcaataat
      481 aaaattaaaa aaggacaacc gactgaaatg agtaattgtc cttaagtggg cccacctggg
      541 ctcgaaccag ggacccacag attatgagtc tgttgctcta accgactgag ctatgggccc
      601 gaaaagaatt ataatttaaa tgaactttga gggctgcaaa agtatatttt tgcacgcaat
      661 aaacaaaaga tagaaagaga atgattaaaa atatcatttt tgattttggt ggggtgttac
      721 ttccgataaa tactgaaaag accgcaattg agtttgctaa aatggggttg gataattttg
      781 aagaactgta ttcaataaat aagcaagcca agttttttga tgatttcgaa aagggactta
      841 ttcctgcctc tgcatttcgc agcgaaataa gaaagcatta taaacgcccg ctttctgatg
      901 aagaaattga ttatgcctgg aatgccttac ttggtgagct gaatcctgag agatttaatt
      961 tcctgagcga acttggtgat aaatacaata tttatttgtt gagtaatacc aacattattc
     1021 atcatgcagc gtttatcaag agtcttaatc aaacatttgg taaagaccgt tttgagaaac
     1081 tgtttgaaaa aacatactat tcttttcaga tgggaacacg caaacccgat aaaagaattt
     1141 ttactgatgt gattaaagac agtggtgtag ttcctgccga aacagttttt attgatgata
     1201 atctgaataa tgcattgggt gcacaaaagg taggcatgaa tgccattcat catgaccatg
     1261 ccatggaaat tgaaaaagaa ctgatggcaa tgctctctgc tttttaagat ttaatttcct
     1321 gacacaattc taccaacaca ccattggttg acttagggtg aagaaaacaa accaatttat
     1381 tatcggcacc tttcttaggc tcactgttta agagtgtaaa acctgattgt tgaagtcgtt
     1441 gcatttctgc tttaatatca tcaacagcaa aggctatatg gtgaattcct tcaccacgtt
     1501 tttcaataaa ttttgcaata gcactttccg gtgaagtagc ttctaaaagt tcaacctttg
     1561 tttcgcccga ctgaaagaat gaagtaatta cattttcgct tacaacgctt tctcttttat
     1621 aaggtggaca accaattaat gcttcaaata aaggatcgga aatttgcaga cttttaactg
     1681 caatgccaat gtgctcaaga ttcttcagtg ccatttttct cgtctgattt tttgtacgaa
     1741 taaacaattt ttaatgatga ctgtggtgca ttatcatcat tttcgtggtc atcaaagggt
     1801 tcatcatcat cagtccactc ataaacttcc tgctgctgac cttcctttct gaaaactatt
     1861 gcacaaagta ttccggacag ggcaccaaat aaatgccctt cccacgaaat ttttggaacc
     1921 acaggaaaaa gtccccaaac taaactaccg tataagaaaa ccataagcag tgaaacagca
     1981 ataagccctg tatgctttct gataaagccg cttaaaaata aaaatgccgt aatgccataa
     2041 acaatattgc tggcaccaat atgatagttt tcgcgtcctc caagccacag ccaaaagcca
     2101 cttaatatat agataagcaa tattaccttc acagccattt ccctgtaaaa ataaaataac
     2161 atactaccgg tcactaacag tggaataaca ttactgaaca aatgctccca gtcggcatga
     2221 aggaatggaa taaataatat tccttttaat ccacttattt gtcttggtaa tactccatac
     2281 acgcctaaat cggtatgcga taagacttca aaaactttta caccaatcat taaaagtgat
     2341 aggaggagtg gtacagtaaa gcttttaaaa atttttcgtg cttccggatg catgtgtttt
     2401 accttacaaa caatctgcca ttaagattaa tcaagaaaaa tctgccttaa tttctgatgc
     2461 tattccttgt ttgtcagtaa aatgttggtt taatgacaat gtgctttgcg aaattgaaat
     2521 atcctgaatg aatgtaaccc aagaagaact atagaataat tttacaattg atccaatcat
     2581 catcaaagac agtattgtac tttttataaa aattaattgc aggctcattc cagttcagta
     2641 cctgccattg caactgttgg cagtttagta acttggcttc tgccgctaca gcatcaaaca
     2701 atttctgacc tattcctttt cctcggagcg attcttgcac aacaatatca tctaaataaa
     2761 gacactttcc tttccatgtt gaatatttga taaagtaaat agcaatgcct gcaatattat
     2821 tttctgtttc tgcaactaac aaatcaaaaa ttggtttttc tccaaagccg tctttaagca
     2881 tttgttcaac ggtgttgata acctgttcag gagctttttc atattctgcc aattctttta
     2941 ttagacgaaa tgccggtgca acatcagctg gagttccttt gcgaatggtt accatatttt
     3001 tataaaattg attttaataa tatttctgag tatgtggtag gaggttgctt aaataatttt
     3061 tctgtttcta aataacaatc ggctttgcca tctttcatca ccaaaacatt atcacacatc
     3121 tgacggataa ctttcaaatc gtgtgaaata aaaatacatc cgaaatgaaa ttgttgttgc
     3181 agactgttta tcaattcaag aattttagcc tgaacagaaa catcaagtgc actcacagct
     3241 tcatcgcaga taagcaaatc gggagcagga atcaaagcac gggcaatgca taatcgttgc
     3301 ttctgtccgg agcttaactc atgtggaaat ctattgaaat gttgttccgt taatcctact
     3361 ttttcaaacc agtcaataat tttgttttta acaattgtgg cattttgtgg tgaatcatgc
     3421 aattgcatca cttcagtaag ttgtctgccc accgtcattt ccggatcgag tgacccatgc
     3481 ggattttgaa atacagcctg gatttttttt cgccattcct gtttggtacg aatcgggtag
     3541 tgcgtaagat cttcttcgtt taattttatt ttgccactgt cagtttcaat caatcgcagt
     3601 aatagtttag ctaatgtagt ttttccggag ccggattcac cagcaatgcc taaagtttca
     3661 ccttgtttta tggtaaagct gatattgtca actgcatttg taatattttt cggactgcgc
     3721 aatagtcctt gccgttggtt gtaactttta tttaaatttt ctactgaaag aataatcttt
     3781 tcaccgataa cactgttttt tttgaatgtt gaaagccggc tttgtaatag ctcttttgta
     3841 taatcttgta taggatgtgc aaatagtgtt tctgtctttc cctgttcaac aatttgccct
     3901 gcatgaacca caagggtacg atgagcaatt agttttactg cttcaaggtc atggctgatg
     3961 aaaagcatgc tcatgttatt ttctgactgt aattgttgca gtaaaacaag aatatctttt
     4021 tgtaccgagg catctaaggc tgttgttggc tcatcagcaa ttaggagtga tggattacaa
     4081 ctaatcgcca ttgctatggc aacacgttgc agctgccctc ctgaaagttg atgcggataa
     4141 cgcttactga tttgcaatgg atttggcaac tttacttttt caaaaagctg caatactttt
     4201 tctattgcat catttttgtt taatgcagtg tgttttcgaa ttgtttctgc tacctgatgc
     4261 ccacaagtaa acagtggatt aaatgcctgc ataggttctt gcattatcat agcaatatga
     4321 cgacctcgaa tggcagttaa ttgtttttcg gttaattgta aaagatcaac aggttgttgc
     4381 aaattgagaa aggcattgcc actaatggaa acgtttttat tattcaataa tccaagtaat
     4441 gccaatgatg aaactgtttt tcctgaacct gattcaccta ccaatcctaa tgtttcgcct
     4501 gacgtaattg aaaaagaaaa ttcgtttaca gcaacaactg ggttgcagtt ttgatgaaaa
     4561 acaactttaa gattttttac ctcaagcatg gctgccaaga taaaaagtaa atgtcaatgt
     4621 ctgaaaatta ttataaggat tttacttcct tgatgttatc cgcactgttc ctgtcaatca
     4681 gtttatgcaa tgggtcaatg ccagcctttg atggtttttc atcaacaata acaacaattg
     4741 tttttctgcc acctgtaaat aattgtttct gcaaatattg ataagtgtgt tttccgttgc
     4801 catcatattt cataatgcca acgtcaatcc agtcattagg tttaacacct gattcattgc
     4861 ccatgctgtc tgcataaacc tttccggtct ctaaatccaa ggtaacctca tacttgttac
     4921 ctatcttttt atattttgat tcaacacatt ttaggtcaaa cagcgtgata cggttaaaca
     4981 tatcctcgac aacttgtttt aaactgtcag gtgttgcatc ggcaatatac ttaacaaact
     5041 gaggtgcttg cacatagggt ggcgactgaa atccatacgt ttttataaat ctgtgcaagg
     5101 cagcattaag cgtatcttca cccaaatagt ccttcattgc atacattacc aaacttcctt
     5161 tttgatagtg aatgtatccc tgaccatcat ttcttgcaat ggcttgttca tttattcttt
     5221 cagtagaacg gcccgataaa taaccatcca actcgtattt aaggaatttt tccatagcat
     5281 ctttcccgaa ttctttttcc atcaccatca gggctgtgta ttgtgccata ctctctacca
     5341 tcaaagttga accctgactg ttggcgccac aaacctgatg cccccaccac tgatgcgcca
     5401 cctcatgtga cgttacatag aatgcattat ctatatctgt gtcttcacta acgtcataaa
     5461 taaatccaat ggcttcgcta aaaggaatgg tgttgggaaa agattgagca aaagtttcgt
     5521 agcgtggaaa ttccaaaata cgaacctgac ggaactgata aggggaaaaa ttcttctgac
     5581 agtaagccaa tgttttttta atggcatgat tcattttttc aatgttccat gaatgtgttt
     5641 tgtgataaaa tatttcaatg ttaattccct gaaatgaatc acgaaaaact tcataacgtg
     5701 cacttaaaaa agagtagaaa tttaccattg gtgcatccat tttgtaatgg aaatagcgcc
     5761 ttccgttttg tgtccattct ttttgaagat atcccggtga aacggcaatc tgatcaggag
     5821 atgtgctgat aatacattca tatcttatcc agtctgcatc atttgctaag taagtattgt
     5881 tacgcgatgc actgtcactg agcaaacgca tggtatgatt ttttctcgga agatcttctt
     5941 tttttctatc gctgttatct gtcagctcaa atgattctga atagccaaat gcaggcagat
     6001 agttgttgtt gataaaagtg ccgttgttga ccaatccaga ttcttcttct gtattggcaa
     6061 atccattgtt aatatattca atgtcgaaat aaactttggt agaatcaccg ggctttaatg
     6121 catgagctaa tttataaata acaaactgct tctgatagtt ttcaacttca tcagccagaa
     6181 tgtcaaaacc aaacctgttg atttttatat tctcatgtgc ccatgttatt aaaaaagtat
     6241 caatcggcaa agctgatttg ttttgtatga tataataacc tttaatggca gcatatcttt
     6301 tttccggcac caaatcaata tttacattta ctgctgtaat gcgtggctga ttaaaatcaa
     6361 ggtatttgtg aaagcgtttt tcatattctg cctgttcatg tctgacagtt ttatcacttt
     6421 taaatttatg caggatgttg gtgttgtaat aaataaaact tcctgatata ataaaaagta
     6481 aaagtaatga ccaaacaatt tttcttcctc tgctgtactg ccattgctgt ttaaaaattt
     6541 gccaccgtgc tttgattcca atttcactgc catggcggtg cagcattact ccaaggataa
     6601 aaagcacaag tcctaaactt ccccaataaa gcaagaacat gtattttgga taaagaaaat
     6661 gtccgtaccc attcatgtct gagtaaggtg ccgaaggaat gttgccatac caaatcattt
     6721 tatgcgttat ccccatagct gctgctgcca ttcttcctac aaaataggct atcatcaagg
     6781 tatggccgat aaatttgttg ttgaccatgc tgtgaatgaa aaatgcaagt aacacaagta
     6841 aaatcaaact tggatactga ctgacaaaaa gtgtttttaa ataaacatca atatcataac
     6901 gataaaatcc tttcaaggtc tgaattaaaa ttcccataaa catgacagtc aactgcagca
     6961 ttgccacaat aaggcacatt gcaaaaaatt tggctgttat taatgcatat cttgaaacag
     7021 gcaatgaatc tgttatcaga tgaatgccgc gttcacgctc tttccatacc aactcacctg
     7081 aatagaatgt gataattatc aaagtaaaca aggtgaagtt tcctgctaaa tcatcaatga
     7141 tggcataggt aacagggtag atggtggtac caaacatttt gcctatttgt ggcatttgac
     7201 caaggataag aatcacacca catatccaga ttgcaataaa tagaacagag cgtatggttc
     7261 caaaaaaatc aaatcgcacc atttgaagcc attgcctcaa tcgtgttgaa aaggaataat
     7321 cttttgaagt ttcaggaagg ttgatgtgac ttaaactttc aacgttaatt ttttcctgat
     7381 tttttctgct tcgcaaaaca aatggactgg tctttgaaaa ctgaaacaac ctaaaggtaa
     7441 aaaaagcccc gattaaagca attcccatcc acaacaaacg attccacaac agataacttt
     7501 ccaatgggat ggtctgaaga tttttttcaa agggggtcca gtagcgtgta atataaaatg
     7561 agggagtgat gccaaacaca tcaagcaaag caaacttagg attgttttca atatcacctg
     7621 ttgccgaggt aagtactaaa taaaaaataa agaaaaccat cccttgcaga tatattgcca
     7681 attgatttct gaaaagcgat ccggtagcaa aaaacattac acccagaata aacacattag
     7741 ggatgatgat gtatagaaat ggaagtacat aagcattcag cataaacggg ccaatcattt
     7801 cctgatcaat ccagggcatc attacaccag ttaagatgcc aaaaggtatt cctaaaaaaa
     7861 taaaaagtga agtaaagtag gatcctaaaa atcgtccacc cagatattgt gctttagata
     7921 tgggtgtggt aaacatgatt tcatgaaaac gatgttcgta atcgcgatac acaggcacac
     7981 ccattactgc agaaacaatc atggttccaa aaagcgtgag aataagcatc acctgataaa
     8041 tggtatatgg tgcattctta tgaatgttac ctactgcacc accaattgta atagcatctg
     8101 tagcagcaaa caaaaagctt atcaaaaaaa gaatagctaa gtaaatgtag gttgcaggcc
     8161 ttctgaaacg gtacgacaac tcaaagcgaa gaatagacca catggcttat ggttttagat
     8221 taccattaag aacagaaaaa tatacatctt ccaaaacggg ctgcgcaatt acgaagtgat
     8281 ggttaagcgg ctcttcgctc caaacatgca gttcaatcag tccagtgtta aatttctgtg
     8341 atattacatt gtattttgtc atcagctctc gtgcagtagg tttgtcaacc tgacaagtcc
     8401 atatttttcc ctgcacttgc ctgattgctt ctgccggatg cccgctaaac atgacctcac
     8461 ctttattgat aatggccatt tgtgaacata attctttaac gtcttcaaca atatgtgtag
     8521 agagaattac aattacctgc tcactgattt cacttaacag attcaggaat ctatttcttt
     8581 ctgttgggtc aagacctgcc gttggttcat cagcaataat aatttttgga ttaccgatta
     8641 atgcctgtgc aataccaaaa cgttgtttca tacctccgct aaaaccacct aaatttttgt
     8701 tgcgcacctc atagagatta gtttgcagta ataggctttt cactacttca tctcgctctt
     8761 ttttaagatg tacacctttt aataatgcca gatgatttaa taattcctga gcagaaatgt
     8821 ttggatatac accaaattcc tgaggcagat aacccaatat tttttttact tcttccttat
     8881 cttcaagtac atttaaatca tctaaaaaaa tattaccact gtctgcattt tgcaatgtag
     8941 ctattgtgcg catcagtgac gatttacctg caccatttgg gcctaataaa ccaaaaaggc
     9001 cgttctttat ctcgaggtta atgtttttga gtgcctgcac tccgttagga tacgttttag
     9061 tcagaccttt tatggtgagt ttcattagca gaaattttgt tgtattacat tatctgaatg
     9121 cacaaaagca agcaaatact attgaaattt attagcggct aaagtaacga agaaaagaaa
     9181 acagcgatgc aaaggttctt ttctgctgat gtgacaccac atctattggt gaaatagtga
     9241 tggaagcaat atagttgttg ccatacttta ctgcaggata aatttgttgg ttgattttat
     9301 ttgcggtaat agttacaaca ggtaattgaa cagctttttg ttcagtagca ttgggagtaa
     9361 tgatgacttc cggaagttgt tggtatggca tcacctcacc ctgactgata tatgcaggaa
     9421 caactacggg tggttgtgag ttttgtgcaa atgcaggcaa tatagctgca actaaaatga
     9481 cggttaaaat tttaaagcgt ttcatctgta tatgttttta ttgattattg cgatgcaaag
     9541 atatatttat aagacagtac tatgcaacac atagtagtat aaaaaattgt attatattga
     9601 aaatcagtca taaaaattta aacttgatta ttaaaatatc ggttttatgc ttttctgaga
     9661 accaataaca agacgattca gatgactaaa atgttacaca agcaggttaa tctatgcata
     9721 gaaaataata aaaaacaacg gacttttatc ggcttagtat taatattgcc gcaacatatt
     9781 tactccatgc atcattattt attttcttat ggtactttgc aacttgagca agttcaactt
     9841 gaaaattatg gccgaaaact aaaaggcagt aacgatttct tgaatggcta taggattgaa
     9901 gacttagtaa ttacagacat tgaagtaatt cgtaaaagtg gtaagcatat tcatcctgtt
     9961 gctgtctgtt ccggaaataa aaacgatgtc attaaaggaa tgatttttga aattactgaa
    10021 gaagaattac aagctaccga taagtatgaa gtgtctgatt atgtacgtgt gatggaaaaa
    10081 tttgaatccg gaaaagaagc ttggatatat attggtaaaa taaaatcata aagcatcaat
    10141 ataccccacg aacctttata gtctctttgc tgttttttaa atcggagatc aaacctgaag
    10201 aagctttttt atctatgtca acagcaacat agccaatcgt attgttggtt tgaagatatt
    10261 gtccaagaat gtttgcttta tgtttactca ttgtagtatt tatttcagaa agtacaccgg
    10321 gcacgttgcg gtgaatatgt aaaatgcgat gtgcactttg ttgcaatggt aaattcaatg
    10381 caggaacaga aacactaccc atgctgttgc ctgtttcaag ataacggata atttttccgg
    10441 ctacatcagt gccgatattt tcttgagctt caagtgttga ccctccaatg tgtggcgtca
    10501 acaatacatt attcaaatgt tgcaagggcg attgaaaaat atcaccttta tgttgcggct
    10561 catcactaaa cacatcggct gcaagtcctg ctaaatgttt ttctgtaatg gcttttgcaa
    10621 tagcaccttc atccataacc tcaccacgac tcaggttaat cagaacactg ccttttttca
    10681 tttttcgtaa aaaagactca ttcaccatat tgcgcgttgc cgaagttccg ggtacatgca
    10741 atgtaacaat gtccgattgt tttaacaagt cattcaaact cttgcaggct tttgcatttc
    10801 ccaaaatcag tttaggttct acatcataaa aaattacctg catacctaat gcctctgcca
    10861 aaacagaaac ctgactgcct atgtgaccat agcctattat ccctaatgtt ttaccgcgaa
    10921 cttcaaaaca acctgcactg tctttcaacc attttccggc aagggctgca ttactttttt
    10981 caggtattct gcgtaacaac ataatgcaat ttgcaatcac taactctgca actgagcgcg
    11041 tattgctgaa aggcgaattg aaaacagcta ttccattttc tatggcatca cccatgctaa
    11101 cctggttaac accaatacaa aaacatcctg ttgcaataag tttatctgcc tgtgcaataa
    11161 cagcagcagt aagttgtgtt tttgaacgta tgccaagtat atggatattg cttattttct
    11221 gcagtaattc tttttcagat aaagcaccgg ttgtggtttc tatttgtgta tatcctgcat
    11281 ctttaaacat ggctacagca gcaggatgaa caccctcaag caaaagaatt ttaatttttt
    11341 ctttcggaaa acttgtcaac gacataaatc aattttgctg caatgatagt ttgttttgtc
    11401 tgaacaatat taagctatgt tggacaagtt atttacattc tgccaggaaa tgataaaacg
    11461 aattaaatta gaggagaaat ataaaaatga ttttataaaa agtatttttt actccctaac
    11521 aattttcatt gttttaactt catctgtaac tgtcgttagc ttaataaagt aaatgccgtt
    11581 tgaatttaaa cttggtttta cttcataaga ttgcttaagc ataagtttta aatcttcaga
    11641 aaatacttta ttgcctaatg tattataaat ttcaattttc gctaacttat ttttagcact
    11701 aatactaaat gtattgctaa aaggattggg tgcaatatca aaatcgaagt tgttttctaa
    11761 agcagaaact gataatgcag ggtcacaaac acctatctga aacggatgca gattatatgc
    11821 tgtgtctgtt gccatagccg tactgtcagt taagattgtt ctgatgtatg aattattact
    11881 tattattcct gaaggcacat tccattgata gttgccattg ttaggtgctg ctgcggcaac
    11941 cgttgaccag ataccgttaa ggccggttga agaaaattca atctttactg atgtttgatg
    12001 tcccggaatg acactgctgt tccatgtaat atttcttact gcattggcag gcatacattc
    12061 atatcctttt ggaaaagtct gattgatgtt aagtgttgtt accggagtaa cttctctgag
    12121 ataaaccaat ttattaatag tcgaaaacgg tccgttgttg aatgttgccc aaactgcaat
    12181 ttcagggtaa ccgttttgat ctgcatcagc aatggctatg tcctggcaaa aagtcatgtt
    12241 ggttaaaggt atagttaaaa cattattcca tacggttcct ccattaccta aaaaaataac
    12301 aatactgcct gtggtagcaa ctactgcatc tgcaaagcca tcattattta aatctgccag
    12361 ttttatacat gatgccaacc cggatactgt taagcctgat gatgaattta cccattgttg
    12421 tgcaacgtta tcccatttgt aaacataagg cacactgttg ttatgaataa agccaaaatc
    12481 atcgtcacca tcgccatcaa tatctttaag agaaatgtca taaaaaccta aaggaccaat
    12541 gggtggaagg ttaaaatgac tgtttgtgaa atttcctgta ccatcaccaa aataagcagc
    12601 accattctga tggcatgaaa caaaatcaag attaccatca tggtttatgt caccacattc
    12661 aacataatgt tctgtgttac cattaatata tccgaatgaa tgattccata ctcctgtccc
    12721 tgagtttaga tatacatgtg tgcctgcaca acaaccaaac gaaccggaaa caatatcgag
    12781 caaaccatca ttattaaaat cgccaaagtc tgttccaaac atgccccaag tttcgcctgc
    12841 cgtagcaaga gagtcgtccc atggagtcca gttcattcct gtgccatcac ctaatgcaac
    12901 ttccagaagt tgatcgccaa aatcagttga agaataattg tgatgaatgc cataacctac
    12961 atctaaaaaa ccatcgttat tgacatcgcc aactgcaatg ccaccataac caaaatcacc
    13021 attctgataa agtgtccagc cggagcctgt tccattacca aaaaaaacgc tgatgccatg
    13081 ttgacttgtg ttgatgagtg ggctgccatg atcgccaaca gtcaataaat ctaaatgtcc
    13141 gtcattgttt atgtcggcaa attcaatttc tgtttgtccg ccttctaatg ttagcgcatt
    13201 aaaactgctg taatctgtaa tgtatgatgg tatctgcgca cgaacagaaa attgtactga
    13261 tacgattgtc aaaaaaagta taagtttttt cataatgaat ggattataaa gcaaaaatat
    13321 gtttttagtt ttaatgtaaa aagaagtgtt tcttattgac tctggtaaaa attttcaggc
    13381 ttatgccgga gataataaac attcctgttg cctatcagcc tttttcttaa ttttgtaaac
    13441 taatctgtaa agaattatgt acacaccaaa acgtgttatc acacttggac aatttattat
    13501 agaacgccag tcagatttcc cttatgctaa aggagaatta tcacgtttac ttcgcgatat
    13561 tggtattgct gccaaaatgg ttaatcgcga agtgaataag gcaggcttgg tagatattct
    13621 tggcgactat ggcaatacca atgtgcaggg cgaagaagta aaaaaattgg atgtatatgc
    13681 caacgaacaa tttatatctg cattgagcag cggtggtgaa acctgtgctg ttgccagtga
    13741 agaaaacgaa gaaatggttc ccatcaattc cgaaatttct aaaaatgcaa agtatgtggt
    13801 tgcaatggat cctttggacg gctcatctaa tattgatgtc aatgtttcta taggaacaat
    13861 cttttctata tacagaagaa caactctttc aggccccagt aacctttctg attttatgca
    13921 aaaagggact gaacaggtag cggccggtta tgttatttat ggctcatcta ccatgatggt
    13981 ttataccacc ggtaaaggcg taaatggatt tacattagat ccttctatcg gtgaattttg
    14041 tctttcgcat cccgatatga aaatacctaa gcagggattt atttattctg taaacgaagg
    14101 taactatgca cattttcctg agggtgtaaa gaagtttatt aaattctgtc aggaggaaga
    14161 tgaaaaatcc aaacgccctt attcatcaag atatatagga tcgttagttg ccgattttca
    14221 ccgtaatctt ttaaaaggtg gcatttatat ttatcccgca acagccaaat caccaaaagg
    14281 aaagctaaga ttactttatg aatgtaatcc attggcattt attgtagagc aggccggagg
    14341 caaagcctct gacggattta accgcatact cgacattcaa ccaacatctt tgcatcagcg
    14401 aacaccattg tttataggat cgccagacat ggtttcaaaa gcagaggagc tgatgatgcg
    14461 attcagtcag gtgagtacat cttaattttt ctaattattc agaccttttt aaagacaaaa
    14521 agtaattgct tacttttgta aaagataaca tcacattatg cgcgaaatac aattcagaga
    14581 agccctgcga gaagccttaa gcgaagaaat gcgcagggac cctaatgttt ttttaatggg
    14641 cgaggaggta gcacaataca atggggcgta taaagtttcg caaggcatgt tggatgagtt
    14701 tggtgccaaa cgtgttattg acacacctat tgcagaattg ggttttgccg gcataggcgt
    14761 tggtgctgcc atgaatggat tacgccctgt tatagagttt atgacattta acttttctct
    14821 cgttgccata gatcaggtca ttaatgcagc agctaaaatg aaaagtatga gtggtggaca
    14881 atatggtgtg cctattgttt ttcgtgggcc tacagcatcg gcaggtatgc tcagttcaca
    14941 acactcgcag gcctttgaaa gttggtatgc caactgcccc ggtttaaaag ttattgttcc
    15001 ttcaaatcct aaagatgcca aaggactgct gaaagctgca atacgtgatg acgaccctgt
    15061 aatttttatg gaaagcgaac agatgtatgg cgacaaaggt gaagtacccg aaggcgaata
    15121 cctgatgcct attggtgttg cagacatcaa acgtgccggc aatgatgtaa ccattgtttc
    15181 gttcggaaaa attataaaga ctgcttataa ggcagcagaa gaattagcca aagaaaatat
    15241 agatgtagaa attattgacc tgcgtactgt acgtcctatt gactatgcca cagtaatagc
    15301 gtctgtgaaa aaaaccaacc gtatggtaat aattgaggag tcgtggccgc tggcaaacat
    15361 tgcaacagaa gtagctttta aagtacagcg cgaagcgttt gactatcttg acgcaccggt
    15421 gctgcgcgtt acaggtggtg atgtgccatt accttatgca cctacactta ttgatgccta
    15481 ccttccaaat cctgagcgta ttatcagagc cgtgaagagg gtgatgtatt tggaataaac
    15541 aaattttgca caacgcaccc ccatattagg attgttttaa ggcttctcaa taaagttata
    15601 aaaatatttt gaatttatta ttttataact atatttgctt tgaattatat tcaaactaaa
    15661 attataaatg tcatgaaaaa gctaacttgt ttgtttgttt gtttgtacaa cattaagagc
    15721 acaaacactt aactttgttt tgtcagtgga taccattgct attggtgaca cacttacggt
    15781 gaacaatact tcatcatacc cacctaatac gatatttgta tggaataatg gcgaatattg
    15841 cagaacaaca aatctgactg gtattgcagg tggagaagtt ctgataaatg atagcctttg
    15901 taatgatact gttattggta gcagccctat taattttata tatgaatt
//
LOCUS       DAOQNP010000073        15660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1335_length_15660_cov_7.954822, whole genome shotgun sequence.
ACCESSION   DAOQNP010000073 DAOQNP010000000
VERSION     DAOQNP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15660
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_15660_cov_7.954822"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1285
                     /locus_tag="PKY09_09905"
     CDS             290..1285
                     /locus_tag="PKY09_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13212.1"
                     /translation="MKRLLLFINLLCFGFVSKGQNLVLNPSFEDTIPCSSYQGPPQLP
                     CSSWYWATGGSVDYFNNYCGDLVFYSVPANAFGFQYAKTGTAYCGIGMYQNPIFPPIG
                     LREYLGGQLSDTLRQGHIYCVSFYVVKSDSGKFYTSDIGMYLSPDSSVDYSTALNLSY
                     TPQIVNTNGIIYDTLNWTQISSTYIAGGGEKYFIIGNFNNDVNTTLDSNNQTNNPNTA
                     FSYYYIDDVSVVDCTVGINEIESYKNKISLMPNPAKDELRISLSLTLPNGAGTIVTQI
                     QKLEITDVLGNVVLIESVKSDEKNIKINVSNFSKGVYFVKVFNDKGVTVKKFVKE"
     gene            complement(1429..2853)
                     /locus_tag="PKY09_09910"
     CDS             complement(1429..2853)
                     /locus_tag="PKY09_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNB13213.1"
                     /translation="MKLPFKKLTTLVLGSFIGPFIMTFFIALFVLLMQFLWKYIDDLV
                     GKGLDWWTITKLLGYTSTTLVPLALPLAILLSSIMTFGNLAEHYEIVATKSAGISLQR
                     LMRPLVVVAIIISLGAFYFSNNILPYSNLKMGSLLYDVRQQKPALFIKEGVFYNGIDG
                     YSIKIGKKAADGKSIKNVMIYDHTHNMGNKKVVIADSGTMVMTNDKRYLILTLINGHS
                     YEEQQNRKKNYDTNPLLRTQFQSETIRFDLSSFQLNRTNEDLFKDNYQMMNVKQLNAM
                     IDTFQLQAAQRKTRFARDIKPYYQFLKDSTLFIHVSNAIAKPFLTDNIKLNNTKFETA
                     LSQCRVIKSYIESADDDLESRNRAYVKYQIEWHRKFTLSIACLLLFLIGAPLGAIIRK
                     GGLGLPIVISIAFFLCYHIISITGEKMAKEGIISVERGMWQSSLVLLPIGIFMVYKAT
                     HDSALFDMDSYKSFFKKLFRKKTA"
     gene            complement(2873..3346)
                     /locus_tag="PKY09_09915"
     CDS             complement(2873..3346)
                     /locus_tag="PKY09_09915"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNB13214.1"
                     /translation="MSNHNHFMEEAVALSKKAITDNEGGPFGCVVVKDGKIIAKGNNK
                     VTSTNDPTAHAEIVAIREACKALNSFQLDDCEIYTSCEPCPMCLGAIYWARPDVVYFA
                     NTRQEAAAIGFDDEFIYNEINIHPTDRKIKMVHLPNQQAAGVFNSWQNKTDKTEY"
     gene            complement(3350..3754)
                     /locus_tag="PKY09_09920"
     CDS             complement(3350..3754)
                     /locus_tag="PKY09_09920"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNB13215.1"
                     /translation="MPTSEVTYISELRVKSVHVFSGNEIITDAPLDNQGKAQAFSPTD
                     LLATSLANCMLTIMGIAARTHQFSIDGTRAEVTKTMASDPRRVSAIEVKLFFPKNNYS
                     DKEKKMIENAAHTCPVAYSLHPDIKQDISFNF"
     gene            complement(3757..5415)
                     /gene="recN"
                     /locus_tag="PKY09_09925"
     CDS             complement(3757..5415)
                     /gene="recN"
                     /locus_tag="PKY09_09925"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNB13216.1"
                     /translation="MLTRLVIKNYVLIRELDINFSDGLSIITGETGAGKSILLGAMGL
                     VLGERADAKTLFDKNNKCIIEAEFSISHYNLKDFFSQHDIDFENDTIIRREINAEGKS
                     RAFVNDTPVNLSVLKELSSFLVDVHSQHQTLTLNTSAFQLNVLDITAGNQKQLNDYNN
                     LFNEYKKLLKQVAELKALENKSIAESEFLQFQFDELEAAGLKSGEFEKIESELQILNN
                     TENIVLTLDSTSDILSEGETNVLSNITQAVNRLNGIARLHPSLTDILKRIQSCNIELK
                     DISSELTALKSQFSLNPSQLEILNDRLSVYNKLLQKHRVKNADDLIVLQQGIASQLQS
                     FQSLADEIEKCEKQTQEIYHSLRLIAEELHNKRQKAAQSFKKDILKLLKEVSLNDAQF
                     DIDLTTTEELTTAGTTAVRFLFSANKGVTVMELAKVASGGELSRLMLCIKALIANKTS
                     MPTIIFDEIDTGISGEVAHKVGNLIEQLSKSRQVLAITHLPQMAGKGKTHYFVFKHIE
                     KGIAQSRIKTLTPDERVVEIAKMLSGEEVTDASLQNARHLLIKS"
     gene            complement(5448..6665)
                     /locus_tag="PKY09_09930"
     CDS             complement(5448..6665)
                     /locus_tag="PKY09_09930"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNB13217.1"
                     /translation="MTPDNTMKLNSIEEALADLKNGKAVIVVDDADRENEGDFITAAR
                     NATPEMINFMASHGRGLICATLTEQRCEELALNMMVATNTSSHETPFTVSVDLLGHGC
                     TTGISASDRSKTIKALIDPATKPSDLGRPGHIFPLKSKNGGVLRRAGHTEAGVDLARL
                     AGFEPAGVLVEIMNEDGTMARLPDLVKIAKKFDLKIISIEDLISYRLKTESLIEREVE
                     VKLPTAYGSFELFAYKQNTTGQEHLALVKGAWKKDEPILVRVHSSCLTGDIFGSCRCD
                     CGPQLHKAMQMIEKEGKGVIVYMNQEGRGIGLLNKLKAYKLQEAGKDTVEANQELGFK
                     PDERDYGVGAQILRDLGVSKLKLMTNNPTKRAGLIGYGLEIVEIIPLEIESNEHNRHY
                     LQTKRDKMGHTIK"
     gene            7224..7649
                     /locus_tag="PKY09_09935"
     CDS             7224..7649
                     /locus_tag="PKY09_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13218.1"
                     /translation="MKKLSLSKTLLTGAVLALMTAGFTACKKDSDPDRKKFLGSFSVT
                     TGTISCGVSGNGTIAAGTAVVITENSGGDEKVNVSVGGQLSVVATANGNTITLDNQTV
                     SGFTYTGTGTLSSNTLTLSINEYDATVPENCVYSMTANK"
     gene            complement(7864..8283)
                     /locus_tag="PKY09_09940"
     CDS             complement(7864..8283)
                     /locus_tag="PKY09_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13219.1"
                     /translation="MKKSLLALSVAGIFLLSACGPSAEEKAAEAKRVQDSIDAVNAAA
                     AEKAYQDSMAMVAEAQAAAEKAAQDSIAAAEEAAKNKKGGKPATKTVTKEEPKKVEPQ
                     KQSGSLKDKIGAETKNATDAGKKEGENAPKKLKDKLK"
     gene            8451..10292
                     /locus_tag="PKY09_09945"
     CDS             8451..10292
                     /locus_tag="PKY09_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNB13220.1"
                     /translation="MSNRKIVLWLLAFLIAIYSLLRLSFIFINFSSFSFSSPAEFLII
                     LFAGLRFDLSAIFITNVLIAVILLLPLKFHQKSARIGITILFLLGNLPFLLFNLVDVG
                     YFHFTQKRTTADFFGVATMGDDFFNMLPSLMRDYWGLLVGFLLLAVVFYKAFQWFLNR
                     RTGKSKWWFVEITVTIGLTVLLFRGGLQYKPISVLTAARYTSNQNVAAVLNTTFTILK
                     TLGKDELKNYDYDKKMPSALTYFNPMHNYANTGVFKRMNVVLIIMESFSKEYIGYFNH
                     GKGYTPFLDSLMQHSLTCTDAFANGKKSIEGIPCIVSGIPALMNTPYISGQYNSNNLS
                     SLPFLLKKYGYSSSFYHGGNNGTMGFENFSKMAGYDYYVGRNEYGDKDYDGTWGVYDE
                     PFFQFFCHELNTQKQPFVATVFSLSSHHPYAIPPQYHSRFKDGEQPIHRAVRYADYAL
                     QQYFECAKKQPWYYNTLFVITADHTGPSIVSHYQTRTGIYEVPIVYFAPDDSLLQGQY
                     ENVTQHTDIVPGILNYLHYPDSFKFFGNSIFDTTVSNRFAVSFLDGTYQLITNNSITT
                     TDFDTEYHFVLRNAKTENDSIKASNTLKVFVSLYNRSMIHNKLTEDE"
     gene            10285..11157
                     /locus_tag="PKY09_09950"
     CDS             10285..11157
                     /locus_tag="PKY09_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNB13221.1"
                     /translation="MNSSKRVLFILNPKAGHGLNEDIVALANEILKKHFNECIFKKTE
                     FAGHATTLAVEAVKNKIDLVVCVGGDGTVNETAQALKYSETALAIIPTGSGNGLARHL
                     HIPMNYKKAIALIIEGKKVAIDTFLVNDRFAVNLAGVGFDALVAHRFAALSVRGLQSY
                     IKTVISSISDFDEMKFTDLLGEQQKAWLLCIANSSQFGNNARIAPYAQVNDGLLDIVL
                     VKRPSIMQMIPFAVKMFNGKLGSSKSYKCIRTKKYTAQFDIAQPIHIDGEPAGMVSSV
                     EVQVIPQSLNIIIA"
     gene            11159..11503
                     /locus_tag="PKY09_09955"
     CDS             11159..11503
                     /locus_tag="PKY09_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNB13222.1"
                     /translation="MSKKKNENSGIVYSTNPDFKYNNENSPDPITLTPAQQDLRVMRD
                     SKQRGGKTVTLITGFVGKEEDIEKLTKMLKSKCGTGGTFKDGEIIIQGDQREKVFDLL
                     LAAGYKVKKAGG"
     gene            complement(11868..13109)
                     /locus_tag="PKY09_09960"
     CDS             complement(11868..13109)
                     /locus_tag="PKY09_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB13223.1"
                     /translation="MNFTILTQYYPPETGAPQNRLSDLALQLKNAGHEITVLTAMPNY
                     PQNEIHDNYKGKYFISESINEIVILRSWIYVGKSRSVIPRLFNYFSFVISSFIWGILK
                     LKKQDVIICESPPLFLGITGWLLSVFKKSKFVFNISDLWPESAEKLGVITNKTLLKMA
                     ERLELFLYKKSFLITGQTQGIVQNIKERTQLNNLHWMPNGISEETLQLNFNKSDCRKV
                     FHLPESAFVLSYAGVIGHAQGLEVILNAATELQQKDIIFVICGDGPELNNLKTLAHSL
                     QLNNIVFTGPVRKTDALQLVAASDAAIIPLKKIDLFKGAIPSKIFENCAMSKPILLGV
                     DGEAKELFINEAQAGIYFEPENATELSSAIEKLFENKMLCATLGQNGKQFVLNKFSRK
                     QIANNFLKILEQKLNNSTTRN"
     gene            complement(13122..14201)
                     /locus_tag="PKY09_09965"
     CDS             complement(13122..14201)
                     /locus_tag="PKY09_09965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNB13224.1"
                     /translation="MRIMLLANADSVHTRKWATALEANGFEVIIFSLKAPHEQWLSQS
                     KIKLIVNNKTKSSGLPLSKVSYISALSSLKKAIREFSPDIVHAHYATSYGLLGYLTRF
                     KPFYISAWGSDVIDFPNKSFLHRWLLKKILNSADKIQSPSNYLKRKIADLTQKNVTVI
                     PIGVDTAIYKPVPVERVFEGVTLGIIKSLEPVYRIHLAIEAFYILKNKYKAEKCYLLI
                     VGGGSLENKLKQQVNDLGLNNFVKFTGRVPQNQVVSLHNAIDIFINVSEYESLGVSVL
                     EAAACGKPAIATNVGGLCEVVKDEVTGYLLYPATAEMLAEKIWYLINHPERMKQMGEQ
                     GRKFVAENFELEKCIKLMIDEYLEK"
     gene            complement(14206..15525)
                     /locus_tag="PKY09_09970"
     CDS             complement(14206..15525)
                     /locus_tag="PKY09_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNB13225.1"
                     /translation="MQFIKIMTATPDCIPANNKYQLLQKILCFFLAFTSFMAVPYSRS
                     IAFDWFIQLPGSYKISLFNLVEIISFTTALFIIRLRILSGIERRILIALLLVMATRVI
                     SLLFAKQYETIQYFSILRFAEVLLGLIILTNLLQSKSNRKIFIVGLTCGLLIECFLGL
                     NMVIQTGGHLRGIFFGIPSYQMMVLFILFLFLLTKSQNLVLIIFAINILLTGIMATQT
                     RAALIQLAVGMIAVIIIAYKQSFFKRFTITLVAVTIVHSITLYFMQSVISAAKDRFED
                     AIKEGKIHLKEDEHSVGGGTIQYRLYLWDKSIGAFLSRPVTGIGSGSFARQLDSLPQK
                     FGVKLDRLDKSTPLSTHNTFLGVLAETGLVGFAAYFFWIASVVGIILKYYRSNHFHSF
                     ASAVAIMLCIFIFSDFWSQQSFLPNMTYLTAFLLAYLRDTIKPGAIV"
BASE COUNT         4783 a         2791 c         2927 g         5159 t
ORIGIN      
        1 acggaacacg caaacacagt tgaaggattt tcaaattaaa gaaggattct aaacttatgt
       61 gcaaacttaa aaaaattaac acccttaaac cttattgtaa attaatttgg atatgaaaag
      121 gaaatcccat taattttaca tatcttaaaa aataacatta ttaccaaact aaaattccag
      181 gtatcatgga attccatatt caaagtcccc tgaaatgcgg gagactttga atggacaaaa
      241 aaatgtggta cagaaattat acattattaa agaataaaaa ggttgtgcaa tgaaaaggtt
      301 gttattattt attaatttat tatgctttgg atttgtcagt aaaggacaga atcttgtatt
      361 aaacccttct tttgaagata ctattccgtg tagttcttat caaggtcctc cgcaattacc
      421 atgtagcagt tggtattggg caaccggagg aagtgttgat tactttaata attattgtgg
      481 tgacttggtt ttttattctg ttccggctaa cgcatttgga tttcaatatg ccaaaacagg
      541 aactgcatat tgtggtattg gaatgtatca aaacccgata tttccaccta taggacttag
      601 agagtattta ggagggcaat taagtgatac cttaaggcag gggcatatct attgcgttag
      661 cttttatgtg gtaaaatcag atagcggcaa gttttataca agtgatatag gaatgtactt
      721 gtcaccagac agctcggttg attacagcac agctttaaat ttatcctata ctcctcagat
      781 agtaaacact aacggaatta tttatgatac cttaaactgg acacaaataa gcagcactta
      841 cattgcgggg gggggggaaa aatattttat cattggtaat tttaataatg atgtaaatac
      901 aacattagat tcaaataatc aaactaataa tcctaacacc gctttttcgt attattacat
      961 agatgatgtt tcggtggtag attgtacggt gggtataaat gaaatagaga gttataagaa
     1021 taaaatatca ttaatgccta atcctgcaaa ggatgaatta agaataagcc tctccctaac
     1081 cctccccaat ggggcgggaa ctattgtaac acaaattcaa aaattggaaa ttacggatgt
     1141 gcttggaaat gtggttttaa ttgaaagcgt aaaatcagat gagaaaaata tcaaaataaa
     1201 tgtgagtaat ttttcaaagg gagtttattt tgtaaaggtt tttaatgata aaggagttac
     1261 agttaaaaag tttgttaagg aatgatgctg cttcgtaggt gcgaagctgt gcttcgttgc
     1321 cttttatttt aatggcttgc agccataatt tttatctctc tttcgctaaa agctaaaata
     1381 atagaatggc acgagcgata cactcgcgcc tacttaaata aaaagtcatc aagccgtttt
     1441 tttccggaac agttttttga aaaatgactt ataagagtcc atatcaaaca aggcggagtc
     1501 gtgtgtggct ttgtaaacca taaaaattcc aataggcaac aacactaatg acgattgcca
     1561 catgcctctt tctacactga tgattccttc ttttgccatc ttttctccag taatagaaat
     1621 gatgtgataa cagaggaaga atgcaattga aattactata ggcagtccta ggccgccttt
     1681 gcggatgata gcacctaaag gtgctccaat taaaaacagc aataaacagg caattgataa
     1741 agtgaatttg cgatgccact caatttgata ttttacatag gcacgatttc tgctctctaa
     1801 atcatcatca gcactttcta tatacgattt gatgacacga cattgcgaca aagctgtttc
     1861 aaatttagtg ttgtttagtt tgatgttatc agttagaaaa ggttttgcaa tggcattgct
     1921 aacatggata aacaatgttg agtcctttaa aaactgataa tatggtttta tgtctctggc
     1981 aaaacgtgtt tttcgctgtg ctgcctgtaa ttgaaaagta tcaatcatgg cattaagctg
     2041 tttgacattc atcatctggt agttgtcttt aaacaggtct tcattggttc tgtttagctg
     2101 aaaagacgat aagtcaaaac gaatagtttc ggattggaat tgcgttctga gtagtggatt
     2161 ggtgtcgtaa ttttttttac ggttttgttg ttcttcgtat gaatgcccat ttattaaagt
     2221 tagaataaga taacgcttat catttgtcat caccatagtg ccactatcgg caatcacaac
     2281 ttttttatta cccatgttgt gtgtgtgatc ataaatcatg acgtttttaa ttgatttacc
     2341 gtcagcagct tttttaccta ttttaatact ataaccatca ataccattgt aaaaaacacc
     2401 ttctttaata aacaatgcag gcttttgctg tcgcacgtca tacagcagcg agcccatttt
     2461 aagattgctg taaggcaaaa tattgtttga aaagtaaaaa gcacctaaag aaattattat
     2521 tgccaccacc actaatgggc gcattaacct ttgtaaagat atacctgctg attttgtggc
     2581 aacaatctca taatgttctg ccaggttgcc aaaagtcatt attgatgaca acagaatggc
     2641 taatggcaag gccaaaggaa ctaaggttgt tgatgtgtag ccaagtagtt tggtgatagt
     2701 ccaccagtca agccctttgc caactaaatc atcaatgtat ttccacaaaa actgcatcaa
     2761 cagcacaaag agtgcaataa aaaaagtcat gataaaagga ccgataaaag aacctaatac
     2821 aagtgtggtg agtttcttaa atggtaattt catcaatgat tggttgctga tatcaatact
     2881 cggttttgtc tgttttattt tgccatgagt taaaaacacc ggcagcttgt tggttcggca
     2941 agtgcaccat ttttattttt ctatcagtgg gatgaatatt tatttcatta taaataaatt
     3001 catcatcaaa acctattgca gcagcttcct gtctggtatt ggcgaagtaa accacatcag
     3061 gtcgcgccca gtaaatagca ccaagacaca tcggacaggg ctcgcaggat gtatatattt
     3121 cgcaatcatc taactgaaaa ctgttgagtg ctttgcatgc ttcacgaata gcaacaattt
     3181 ctgcatgtgc tgttgggtcg ttggtagagg taactttatt atttccttta gcaataattt
     3241 ttccatcttt aacaacaacg cagccaaaag ggccaccttc attgtcagtg attgctttct
     3301 tggacaaggc tacggcctct tccataaaat gattgtgatt actcattaat taaaaattaa
     3361 aactgatatc ttgtttaata tccggatgga ggctgtatgc cacagggcag gtgtgtgctg
     3421 cattttctat cattttcttt tccttatccg aataattatt tttcggaaaa aataatttca
     3481 cttcaattgc actcactctt cttgggtcag aggccatagt ttttgttact tctgcacgtg
     3541 tgccatcaat tgaaaactga tgtgttcttg cagcaatacc cataatggtg agcatgcagt
     3601 tggcaagtga tgttgctaat aagtcggtgg gggagaaggc ctgagctttt ccctgattgt
     3661 cgagaggtgc atcggtgata atttcatttc ccgaaaaaac atgaaccgat ttcactctta
     3721 attcgcttat ataggtaact tctgatgtgg gcataattat gattttataa gtagatgtcg
     3781 tgcattttgg aggcttgcat ctgtaacctc ttcaccgctc aacatttttg caatttcaac
     3841 aactctttcg tcaggagtaa gtgtttttat tcttgattga gcaatacctt tttcaatatg
     3901 cttaaaaaca aagtaatgcg ttttaccttt tccggccatt tgtggcagat gtgttatggc
     3961 aagaacttgc ctgctttttg aaagttgttc aatgaggtta ccaactttat gagccacctc
     4021 accggagatg cctgtgtcaa tctcatcaaa gatgatggtt ggcatggatg ttttgttggc
     4081 gatgagtgct ttaatacata acatcagtcg tgaaagttca ccaccggaag ccaccttcgc
     4141 caattccatt accgttactc ccttgtttgc agaaaataaa aagcgcacag ccgttgtacc
     4201 tgcggttgtc aactcttctg tggttgtaag gtcaatatca aactgagcat cgttgagtga
     4261 aacttctttc aacaatttta aaatatcttt tttgaaactt tgtgcagctt tctgtctttt
     4321 gttatgcagt tcctctgcaa ttagtctaag gctgtgataa atctcttgag tttgtttttc
     4381 gcacttctca atttcatctg caagagattg aaaagattga agctggctgg caattccctg
     4441 ctgcagcact atcaggtcgt cagcattttt tactctgtgc ttttgaagta atttgttata
     4501 aaccgaaagt ctgtcattga gtatttcaag ttgcgaaggg tttaaactaa actgactttt
     4561 aagtgcagtt aattccgatg aaatatcttt cagttcaatg ttacatgact gaattctttt
     4621 aagaatatct gttagcgatg gatgtaatcg tgcaatacca tttaaacggt taacagcttg
     4681 tgtaatattt gaaagtacat ttgtctcacc ttcagacaag atatcacttg tgctgtcaag
     4741 tgtcaacaca atattttcag tattgttaag tatttgtagt tccgattcaa ttttttcaaa
     4801 ctcccctgac tttaagcccg cagcttcaag ctcatcaaac tgaaactgca gaaattctga
     4861 ttcagcaata ctcttatttt ccaacgcttt caattcggca acttgcttca ataatttttt
     4921 atactcattg aatagattgt tgtaatcatt taactgcttc tgattgcccg ctgtaatatc
     4981 caacacattt aattgaaatg ctgaagtgtt taatgtcagc gtctgatgtt gtgaatgaac
     5041 atcaaccaaa aaactgctga gttctttaag aacagaaagg tttacaggag tgtcatttac
     5101 aaatgcacgc gatttacctt cagcattaat ttctcttctg atgatggtgt cgttttcaaa
     5161 atctatgtca tgctgcgaaa aaaaatcttt cagattgtaa tgcgaaattg aaaattcagc
     5221 ttcgattata cacttgttgt ttttatcgaa aagtgttttt gcatctgcac gttctcctaa
     5281 gacaagcccc attgcaccca gcaggataga ctttcctgct ccggtttcgc ctgtaataat
     5341 actcagccca tcagaaaaat taatatccaa ttctctgata agcacataat ttttaatgac
     5401 aaggcgggtt aacatgcaat gtttttcttt aaataaaact cggttgatta cttaatagtg
     5461 tgtcccattt tatcacgctt tgtctgcagg taatgacggt tatgttcatt ggactcgatt
     5521 tctagcggta taatttcaac aatctccaag ccatatccga tgaggcctgc tcgtttggta
     5581 gggttgttgg tcataagttt tagtttggaa acacctaaat cacgcagaat ttgtgcgcca
     5641 acgccataat ctctctcatc cggtttaaaa cccagttcct gattggcttc aacggtatct
     5701 tttcctgctt cctgtagttt ataggctttt agtttattca ataatccgat tccacgtcct
     5761 tcctgattca tataaacaat gacacctttt ccttcttttt caatcatctg catggcttta
     5821 tgcaattgtg gtccgcagtc gcagcgacat gagccaaaaa tatcaccggt aaggcaggag
     5881 gagtgaaccc tcactaaaat aggttcgtct tttttccaag caccttttac taaggcaaga
     5941 tgctcttgtc ctgttgtatt ttgtttatag gcgaaaagct caaaactacc ataggcagta
     6001 ggaagtttca cctccacttc gcgttcaatg agtgattctg ttttcagtct ataggaaata
     6061 aggtcctcaa tagaaattat tttcagatca aatttcttgg caatttttac cagatcgggt
     6121 agtcgagcca tcgttccatc ttcgttcata atctcaacca aaacaccggc aggctcaaat
     6181 ccggcaagac gggcaaggtc aacaccggct tcagtatgtc cagctcttct taaaacacca
     6241 ccatttttac ttttcagtgg gaaaatatgt ccggggcggc caaggtctga aggctttgtg
     6301 gccgggtcaa ttaatgcttt aatggtttta gagcggtcgg aggctgaaat ccctgtggtg
     6361 catccatgtc cgagcaaatc aacagaaacg gtaaatggtg tttcgtgaga ggaagtattt
     6421 gttgccacca tcatgttcaa cgccaactct tcgcagcgtt gttctgtaag ggtagcacat
     6481 attaaacctc tgccatgact ggccataaaa tttatcattt caggagtagc attgcgggca
     6541 gcagtaataa agtctccctc attttcgcgg tcggcatcat ctaccactat caccgctttg
     6601 ccatttttaa gatcggctaa agcttcttct atactattca atttcattgt attgtcagga
     6661 gtcattgtct ctgtatctgt ttttgcaaat ttacaacatg cttttcaatg tttaattaaa
     6721 attcttacat ctcaatttgg ttggtttttg ccattattct gtatgttaag atgaagtgaa
     6781 gattgtaata ggtttataat cttacatacg taatgtgcgt ttatagtaat gtaacaaaag
     6841 gcgtaaggcc tgcgttaaat tgctcatatc gttcttgata aagattatta ctaagatagt
     6901 ttggttttag gttaatatta aaaatgattc agccttagcc ctgtaaggct aaaagttgaa
     6961 tcacctaagc aaaaaatagt tttaggttgg tttcaggtgt gggctgcccc gtaagacagc
     7021 ccgtcatctg cattttaaat tcggatttgg ttttagggtt atacttttaa aaatcaccgc
     7081 caacgtggcg gtgatttttt gcttttaatg ggttttaggg ttttcacatt taaacgtgta
     7141 taactttcag ttaaaaaaaa gtgtcttggt aagataattc atgctatatt tgcccgcata
     7201 aacattataa actttattaa aaaatgaaaa aattaagctt atcaaaaaca cttttgacag
     7261 gtgctgtatt agctctaatg acagcaggat ttaccgcatg taaaaaagac agcgatcctg
     7321 atcgtaagaa attcttgggt tctttctctg taacaacagg aacaatttct tgtggtgttt
     7381 ctggaaacgg aacaattgca gcaggtactg ctgtagttat tacagaaaat tcaggtggtg
     7441 atgaaaaagt aaacgttagc gttggtggcc aattgtcagt agttgctact gcaaacggta
     7501 acacaattac ccttgacaac caaactgtaa gtggatttac ttacacaggt acaggtacat
     7561 taagcagcaa cacactaaca ttgtcaatca atgagtatga tgctacagtt cctgagaatt
     7621 gcgtttatag catgactgct aacaaataat tgcttagctt aaagtataaa aaaagagggc
     7681 attgccctct ttttttgttt aagtcagttt gtatttttta atgagattga actttctgca
     7741 cttgatattt ttcaatacaa aaagtatggt tgaagtggtg tccaatatta ttagtgtgtg
     7801 acccaaacaa aaaagaggct gtctcaaaag taggcagcct cttttgtatt acttaagata
     7861 aaattatttc aatttatctt ttagcttctt aggtgcgttt tcgccttctt ttttcccagc
     7921 atctgttgca ttttttgttt ctgcaccaat cttatctttt aaagaaccac tttgtttctg
     7981 tggctcaacc tttttaggct cttccttagt tactgtttta gtagccggtt ttccaccttt
     8041 tttgttttta gcagcttctt cagcggcagc aatagaatct tgtgcagctt tttcagcagc
     8101 agcttgtgct tctgcaacca ttgccataga atcctgatag gctttctcag cagcagccgc
     8161 atttacagca tcaatagaat cttgtactct tttagcctca gcggcttttt cttctgcgct
     8221 tggtccgcag gcgctcagca gaaatatacc tgctacagat agcgctagta gtgatttttt
     8281 catttttatt ttgaattgat tgaaatttgt cgcaaaatta acactttaat agtcaggttt
     8341 tcaatatttt ttcgatatta gatattaaca gtataaagtt aatggttatt aacactaaaa
     8401 ccaccggatt ctaatggtta gtaggcctat aagctatctt tgcccgctgc atgtcgaaca
     8461 gaaaaattgt tttgtggtta ttggcattcc tcattgccat atattcttta ttacgtcttt
     8521 ctttcatctt tataaacttt agctctttca gtttcagcag ccctgcagaa tttttaataa
     8581 tactttttgc cggccttcgc tttgacctta gtgctatttt tattaccaac gtattaattg
     8641 ccgtcatatt actgcttccg cttaaatttc atcagaaatc tgccagaatt ggtataacaa
     8701 tcttgttctt actcggaaac cttccatttc tgctctttaa tttagtagat gttggttatt
     8761 ttcattttac acaaaaacgt acaacagccg atttttttgg tgtagccacc atgggtgacg
     8821 atttcttcaa tatgttgcca tcattgatgc gtgattattg ggggttgttg gttggatttc
     8881 tgttgcttgc tgttgtattt tataaggcat ttcaatggtt tttaaacaga agaaccggaa
     8941 aaagtaaatg gtggtttgta gaaattactg tgacaatagg tttaactgtt ttgttgttca
     9001 gaggcgggct gcagtataag ccaatttcag tactgactgc tgcacgatat acgagtaatc
     9061 agaatgtagc tgccgttcta aatactacat ttactattct aaaaactctt ggaaaagatg
     9121 aactgaagaa ctacgactat gacaaaaaga tgccttcagc cttgacatac tttaatccca
     9181 tgcataatta tgcaaatacc ggagtattta aacgaatgaa tgttgtatta atcatcatgg
     9241 aaagttttag taaagaatat ataggctatt ttaatcacgg caaaggctac acaccatttt
     9301 tagactcgtt aatgcaacac agtctgacct gtaccgatgc atttgccaat ggcaagaaat
     9361 ctattgaagg cattccgtgt attgtttcag gaattcctgc attgatgaat acaccataca
     9421 tcagcggaca gtataacagc aacaacctga gtagcctgcc tttcttactg aaaaaatatg
     9481 gttattcttc ttcgttttat catggaggga ataatggcac aatgggtttt gagaacttta
     9541 gtaaaatggc cggatatgat tattatgtag gtagaaatga gtatggcgac aaagattatg
     9601 atggcacatg gggtgtttat gatgaacctt tctttcagtt tttttgtcat gaactaaata
     9661 cgcaaaagca accttttgtt gctactgttt tttcgctttc atcacatcat ccttatgcta
     9721 taccaccaca gtaccattca agatttaaag atggtgaaca gcctatacat cgtgctgtgc
     9781 gttatgcaga ttatgcacta cagcaatatt ttgaatgtgc aaaaaaacaa ccgtggtatt
     9841 acaacacact ttttgtgatt actgcagatc atacaggccc ttcaatagtt agtcattatc
     9901 aaacacgaac aggtatttac gaggtgccga ttgtatattt tgctcctgat gatagcttgt
     9961 tgcaagggca atatgaaaac gttacgcaac ataccgatat tgttcccgga atattaaatt
    10021 atctacatta tcctgacagc tttaaatttt tcggcaatag tatttttgat acaacagtca
    10081 gcaatcgctt tgcggttagt tttcttgatg gcacctatca gctgattaca aacaacagca
    10141 ttaccacgac tgactttgac acagagtatc attttgtact tcgtaatgct aaaacagaga
    10201 atgattcaat taaagcttca aatacattaa aggtttttgt tagtttgtat aacaggagca
    10261 tgattcacaa taaacttaca gaagatgaat agcagtaaac gcgtattgtt tattctgaat
    10321 cccaaagcag ggcatggact taatgaggat attgttgctt tggctaatga aattctaaaa
    10381 aagcatttta acgaatgtat ttttaagaaa acagaatttg caggacatgc aactacactt
    10441 gcagttgaag cagtgaaaaa taaaattgat ttagttgttt gtgttggtgg cgatggcact
    10501 gtaaacgaga cagcacaggc attgaagtat agcgaaacag cattggctat aataccaaca
    10561 ggctctggca atggactagc gcgtcatttg cacataccaa tgaattataa aaaagctatt
    10621 gcgttaatta tcgaaggaaa aaaggttgcc attgatactt ttctggttaa tgacaggttt
    10681 gccgtaaacc ttgcaggagt aggttttgat gcacttgttg cacatcgttt tgcagcactt
    10741 agtgtaagag gattacaatc gtatatcaaa actgtcataa gcagtatttc tgattttgat
    10801 gaaatgaagt ttactgattt attgggagag caacaaaaag catggttgct gtgtattgcc
    10861 aacagttcgc aatttggtaa taatgcgcgc attgcacctt atgcacaggt caacgatggt
    10921 ttgcttgata ttgtattggt aaaacggcca tctataatgc agatgatacc ttttgctgtt
    10981 aaaatgttta atggtaagtt agggtcttcc aaaagctaca aatgcataag gacaaaaaaa
    11041 tatactgcac aatttgatat tgctcagccg atacatatag atggcgaacc ggcaggaatg
    11101 gtttcatcag ttgaggtaca agtaattcca caatcattaa atataataat tgcgtaaaat
    11161 gtcaaagaaa aaaaacgaaa actcaggaat cgtctatagc accaatcctg attttaaata
    11221 taataatgag aatagtcctg atcctataac actgacacca gcacagcagg atttaagagt
    11281 tatgagagat agcaagcaac gtggcggcaa aacggttaca ttgattaccg gtttcgttgg
    11341 aaaagaagaa gatattgaaa agctcacaaa gatgcttaaa tccaagtgtg gtacaggagg
    11401 aacttttaaa gatggtgaaa taattattca gggtgatcaa agagaaaaag tttttgattt
    11461 attgcttgca gcaggatata aggtaaaaaa ggcaggagga tagtataaaa aaagccacca
    11521 gcgaactgat ggcttttttg aggtcgagag tagatttgaa ctactgtaaa aagtcttgcg
    11581 gacttttgcc tatactctcg gccactcgac cttatgatgt tgaatatatt tcaacgttaa
    11641 caaagtaatt aggtttcatt aaaataacaa aatgtttttg ggtgaatggc cttttttatt
    11701 tggtaaacgg attttaactt gattttactt cacaaattgg ataattgctt aatcttaagt
    11761 ttactttgat tctactcaaa acagagtatt gttttaagaa gaaattattt tatgaaaaag
    11821 aggcttttaa ttttgtattt gttctggttt gaatgcgaaa tatatactca atttcttgta
    11881 gtggagttgt tcagtttttg ctccaatatc ttcagaaaat tatttgcaat ctgttttctt
    11941 gaaaatttgt ttagcacaaa ttgttttccg ttttgcccta atgttgcaca gagcattttg
    12001 ttttcaaaaa gtttctctat tgcagaagaa agttcagttg cattttccgg ttcaaaataa
    12061 attccagcct gcgcctcgtt gataaataat tcttttgctt cgccatcaac acccagtaaa
    12121 atgggtttag acattgcgca gttttcaaaa attttagaag gaatagcgcc tttaaacaga
    12181 tctatttttt taagtggaat aatggctgca tcagaagctg ctaccaattg cagtgcatct
    12241 gttttgcgta ccgggcctgt gaaaacaata ttatttaact gaagcgaatg cgccaatgtt
    12301 tttaggttat tcaattcagg gccatcacca caaattacaa aaataatatc cttttgttgc
    12361 agttctgttg ctgcatttaa aattacttca aggccttgtg catgaccaat aacacctgca
    12421 taagaaagta caaaagcact ttcaggtaaa tgaaatactt ttcggcagtc gcttttgttg
    12481 aaatttagtt gaagtgtttc ttccgaaata ccatttggca tccaatgtaa attatttaac
    12541 tgtgttcgct cttttatgtt ttgaacaata ccctgtgttt ggccggtaat gagaaaagat
    12601 tttttataaa gaaagagctc aagtctctcg gccattttca aaagagtttt attggtaatg
    12661 acaccaagtt tttcggctga ctcaggccat aggtctgaga tattaaacac aaattttgat
    12721 ttcttgaaaa cagaaagcag ccatcctgtt attccaagaa acaatggggg gctttcacaa
    12781 ataatgacat cttgtttttt aagttttaaa atgccccaga tgaaagaaga aatcacaaac
    12841 gaaaaataat taaacagtcg cggaataaca gagcggcttt taccgacata tatccaactt
    12901 ctgagaatta caatttcatt aatgctctct gaaataaaat acttgccttt atagttatcg
    12961 tgaatttcat tctgcggata attaggcatt gctgtaagaa cggttatttc atgtcctgca
    13021 tttttcaatt gcaacgcaag gtcagaaagg cgattttgcg gagcacctgt ctcaggaggg
    13081 taatattggg tgaggatggt aaagttcatt tttatgccac actatttttc aaggtattca
    13141 tctatcatta gtttaatgca cttttccagt tcaaagtttt ctgctacaaa ctttcgtccc
    13201 tgttcaccca tttgcttcat tctttccgga tggtttatca gataccagat tttttctgcc
    13261 agcatttcgg cagtggccgg atatagtaaa tatcctgtaa cttcgtcttt gactacttcg
    13321 caaagtccgc ctacgtttgt tgcaattgcc ggtttgccgc aagcagcagc ttccagcacg
    13381 gaaactccca ggctttcgta ttccgaaaca ttaatgaaaa tatcaatggc attgtgtaaa
    13441 gaaactactt ggttttgtgg cactcttccg gtaaatttta caaagttatt taaacctaag
    13501 tcatttacct gttgctttaa tttattttct aaagaaccac cgccaactat cagcaaataa
    13561 catttttcgg ctttgtattt gtttttcaga atgtaaaatg cttcaatggc taaatggatt
    13621 ctgtaaacag gttcaagcga tttgatgatg cctaaagtta ctccttcaaa aactctttca
    13681 acgggcactg gtttataaat agcagtatct acaccaatag gtatgaccgt gacattcttt
    13741 tgagttaaat ctgctatttt tctttttaag tagttactgg gactttgaat tttatcggca
    13801 ctgttcagga tttttttcag cagccatcta tgtaaaaaac ttttattggg gaaatcaata
    13861 acatcactac cccaagcaga aatataaaaa ggtttaaacc tggtgaggta gcccaacaag
    13921 ccataacttg tggcatagtg agcatgaaca atatctggac taaactcacg gatagctttt
    13981 ttcagggatg ataaagcaga aatgtaagag acttttgaaa gtggcaggcc actgcttttg
    14041 gttttattgt ttacaatgag tttgattttg gattgagata accattgttc atgtggggct
    14101 ttcaaagaaa aaataattac ttcaaatcca ttagcctcaa gagcagttgc ccattttctg
    14161 gtgtgaacag aatcagcatt agccaataac attattctca taccttcaaa ctattgcacc
    14221 aggtttaatt gtgtcgcgta gataggctaa aagaaaagcc gtcagatagg tcatgttggg
    14281 taagaaggat tgctgtgacc aaaagtctga aaaaataaat atacacagca tgattgctac
    14341 tgcacttgca aagctatgaa agtgattact gcgataatac tttaaaataa tacctactac
    14401 agaggcaatc caaaaaaaat aggctgcaaa ccccaccaat cctgtttcgg ctaacacacc
    14461 taaaaatgtg ttatgcgtac ttagtggcgt gcttttatcc agcctgtcaa gtttgactcc
    14521 gaacttctgt ggcaacgaat ccagttgacg ggcaaaactg ccactgccaa tacctgttac
    14581 cggccggctc agaaaggctc caatactttt atcccataaa tacaatctat attgtatggt
    14641 gccaccacca actgaatgtt cgtcttcttt caggtgaatt tttccttctt tgatagcatc
    14701 ttcaaatcta tcctttgctg cagagataac tgattgcatg aagtaaagag taatgctatg
    14761 aacaattgtc acagctacta acgttattgt aaatctttta aaaaaacttt gtttgtacgc
    14821 aattatgata actgcaatca tgcctactgc caattggatt aatgctgcac gtgtttgggt
    14881 agccattatt ccggtaagta aaatattgat ggcaaatatt attaaaacta aattctgtga
    14941 ttttgtaagt aagaataaaa acagaatgaa aagcaccatc atctgatatg atggtattcc
    15001 gaagaaaatg cctcttaaat gtcctcctgt ttgaatgacc atgtttaagc caagaaaaca
    15061 ttcaatcaat aaaccacatg ttaatccaac aataaaaatc tttctgttgg atttagattg
    15121 taacaagttg gtcaggataa tcaaacctaa cagaacctct gcaaacctca aaatgctgaa
    15181 gtattgaatg gtttcatatt gttttgcaaa gaggagcgaa attactctgg ttgccatcac
    15241 aagtaaaagg gcaatcagaa ttcttctttc tatacctgat aatatccgaa gtctgataat
    15301 gaaaagagca gttgtgaaac ttattatttc aactaagtta aaaaggctaa tcttatagct
    15361 acctggcagt tgaataaacc agtcgaatgc aatacttctg gaatagggca ctgccataaa
    15421 agatgtaaac gccagaaaga agcataatat cttttgtaac agttgatact tgttgttggc
    15481 cggtatgcaa tcaggagttg cagtcatgat cttaatgaac tgcaaaagta aaaattaggt
    15541 tatatccgtg atagagcatt acattgaatc atccctgatg ccagaggtta caatggaaat
    15601 acggtaaatc caataaatta cataaatgag ataaactgat tgtagtgctg taaataatgt
//
LOCUS       DAOQNP010000074        15448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1362_length_15448_cov_7.091860, whole genome shotgun sequence.
ACCESSION   DAOQNP010000074 DAOQNP010000000
VERSION     DAOQNP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 15448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15448
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1362_length_15448_cov_7.091860"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKY09_09975"
     CDS             <1..538
                     /locus_tag="PKY09_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WcaF family extracellular polysaccharide
                     biosynthesis acetyltransferase"
                     /protein_id="HNB13226.1"
                     /translation="KFNPSEFDRGASSLKFFLWHWVNVIFFISPFCPSSAIRCFLLRL
                     FGARIGKGVVISKPGVNIKFPWRLTIGNHVWIGENAWIYNLDQVTIGNHVNIAQGAMI
                     LTGNHNYKSSKFETIFGPVTIEDGVFIGAKAIVCPNVTCYSHSILAAGSVATTALKAY
                     GIYQGNPAEYKKERVIQE"
     gene            815..5032
                     /locus_tag="PKY09_09980"
     CDS             815..5032
                     /locus_tag="PKY09_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB13227.1"
                     /translation="MLFLLLPCFLQAQYDVVGNTILVDWSSAGTSTLSVNYTNTLLNC
                     GGTANITVETKQPFSISGPTKSCPGADVVLAAINPDNVLLDWTLTDASQNTIYLGSGT
                     GVPDFTIAGWTYPSGTYIITALDNTNSYCNYTASITIEITDSPPSPPIPTGDNPICPG
                     TVHLYSGTTLNAGYYLEWSTTGGTPATGTGNEISIGWINTAPPHNYSISLVQVESSTG
                     CKSMPTVLPIQERFYINPVIAGNFTPCINKQVTYQISNAAPLGNYIYDQLEWVIIPST
                     AGSVISGQGTTQITVQWNNQPSPSGVYDTYLQVKTSLCGSPPIPYPNTVFDPPNISLG
                     GAPSLAITASPNPACENTAITFTAVPALPGNYTWSFGDGNSGNSPLTVSHSYALTQGA
                     NLTTFDVTLTVDDGGCQSYASTKVDVNPAPLYSLTISPVAFCPPTTTNITATLVQQSG
                     SFNYSWSNNSSNNTNTNTLNYLDTQPFGITITNTVTGCTTSQTISISTCSPNPLPCNP
                     NPINISFTATPASDCQTIDFTESFNPTPASFLWDFGDGFTSSQANPTHTYSTAGQYGA
                     CLYAYDNTGQQYCDYYCLPISLALFPDFTTSLHCSSTPGSYDVEFIDLSAFINPITSW
                     EWYSGTTLLSTSNHPTITLAAGTYSIKLKIGNGTKYCERTLTINIPAPPVASFTVAGG
                     PFCEAQTVVQLNAGNTNVHHALWDFGDASTFIPQNPAASINALKVFGTQGIYSVTLVV
                     TDNYGCTYTSTQQVAVNDNQLGGVISPVTVSFCPGASGSLELDIITTPGPYTQPTSWL
                     WTGGITTPTLTVSQTGQYFVTVSDNNGCSYSPAKPGQAAVVNVPKPVIWGDLTLCEGD
                     AINLDGNQGSAVQYNWTVVDPNNVTTTFSQSSIHIAGAVAGTYHVTLDITANGCSRST
                     SVDVDVNSLPPLPDIQSSVAQACEGQPIDLTVMNAGTYWVNWSNGASGPSVTVYNAGG
                     YQATLTDANGCTNHATFEVHENPYSGFMMTGCVQYCDTLPVTVFGSGNLSFEKWIWFV
                     DGVAQTSGQQSPVQNLNMGLLSPGVYVVTLQLVVAYPDGTNCEMMSDALEITIIPCPC
                     RLYPEGKIYCMNEVNGDVQSLNNYHFEIVTNYSCSANPNVMVTSPDGGVSMLPNSNPP
                     QLLTGILSTYSAYPNNVCFDINITDSNKPECSCNYLYCMKLPKCGVPVGCGTEIKIDN
                     VQCIGHDANGNRIYSFTLTLLNSPQLFSMFTAQGGVLGTMPATVGPGTTIINSSITVN
                     NNASTFCITLHGYNFTTYTACKTSGCSGNLPPCGTPPSRNGEKNTTVPLLRVSYQNDG
                     NDAVQISTLSLMPNPAKNSVTLRYSARTGGIITITSAVGQKMYNEKVTTQGLSELSTQ
                     PWLPGIYFVTLQSNAGDCLVQKLIIIK"
     gene            5041..6768
                     /locus_tag="PKY09_09985"
     CDS             5041..6768
                     /locus_tag="PKY09_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13228.1"
                     /translation="MKTSVKLFLFFMMLFANATAQTPGWQWLSRVGGYSGNGNGGSGP
                     DEWVKDVKTDAQGNVYVCGRVCYGANFNGDTMTTYQGYFTLFMAKLNCEGNLVWVRTA
                     GSYGNTADGEAYGLALDDYGNIYLTGYISANQFSPTQFFDTLITEETYDFFLAKFDTS
                     GNYKWAKIAGPGPGVTGAGGFKIQITDDDMINVSGTGGGGSSGVFFPGYNISLSWCFS
                     ARFDTSGNINRLQNLGSVYGPRQNDYKISPTGDQYITGFFYLDSMVIGDTVLYKPYNA
                     ACPTCCNIFLVKFDSTGQFQWARNFGDTVYSFNKGYGLSMFGPDILLTGGLNTAANID
                     GFSPTNSLSTNIYMDIPFVAKFNSNGVCTWFTNSQHKYGSWATGGITTLSNGVSYYSG
                     FFGGPAKFGTDSFASTGLTDIFLAEVSPSGVITSGTKITSMGDGDEPQCITHDSSGNV
                     YVGGSNRSSLTLNGVTYPFQGGESDGFIAKWGTNCTVGIAEQESMLAEDLFLYPNPAT
                     TEINIQNKVQGITNITVYDVVGKKILSKKILSKELLVKIDVSSFSPGMYIVKATGNGY
                     LSTAKFVRE"
     gene            complement(6793..7788)
                     /locus_tag="PKY09_09990"
     CDS             complement(6793..7788)
                     /locus_tag="PKY09_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13229.1"
                     /translation="MKFNGHYIVLFSMLLFAACTNGKKETEESKDKIAQLTENIKADS
                     TNPKNYFDRATAYSQKSMFDLAYFDMEKAIKINPSDPEYLMLYSDLSFRMNKVKDARN
                     ALVKVRQMQPSNFDAAFKLAEIFLYTNNQEQSLLYLDTVLQKDPKNTKALLMQGFNLK
                     EKKDTAAAIASFRNAVAIDPNFYEAQIQLGLLMYLQNNKLCVDYYTNALNIKANSEEA
                     MYGLALWYQDHQEYNKAIQYYTEIIQINPKNKNAHFNLGYIHHEYLKVYDQAIKHYTD
                     AINADPNYAEAYYNRGLCYEYVGNITAAKEDYTKALQLRQNNYPHAKEGLERVSR"
     gene            complement(7886..8899)
                     /gene="recA"
                     /locus_tag="PKY09_09995"
     CDS             complement(7886..8899)
                     /gene="recA"
                     /locus_tag="PKY09_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNB13230.1"
                     /translation="MSTVQAPDKEKLKVLQAAIERIDKTYGKGTIMRMGEEAIEPVEA
                     IQTGSISLDIALGINGLPKGRVIEIYGPESSGKTTLAIHAIANVQKNGGIAAYIDAEH
                     AFDRFYAQKLGCDIENLLISQPDNGEQALEIADNLIRSGAIDIIVIDSVAALTPKSEI
                     EGEMGDSKMGLQARLMSQALRKLTASISRTKCCCIFINQLREKIGVMFGNPETTTGGN
                     ALKYYASVRLDIRRIGQIKDGDSVAGNRTRVKVVKNKVAPPFRQAEFDIMYGEGISKT
                     GELVDLGVELNIVKKSGSWFSYGDTKLGQGRDAVKQLFADNPELADEIENKIKEAMNL
                     KTA"
     gene            complement(9189..9650)
                     /gene="smpB"
                     /locus_tag="PKY09_10000"
     CDS             complement(9189..9650)
                     /gene="smpB"
                     /locus_tag="PKY09_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNB13231.1"
                     /translation="MAKKDFAPETVNKKASFDYFFEQKFEAGIVLTGTEVKSLRTGQV
                     NLTDAYCVFKNGELWVKNIHIAEYKQGGYANHIAKSDRKLLMHKRELEKLQTKLKTKG
                     TTLIPTRIYFNEKGLAKLEIALARGKKMFDKRQTEKEQEAKREVARVMKRF"
     gene            complement(9670..11544)
                     /locus_tag="PKY09_10005"
     CDS             complement(9670..11544)
                     /locus_tag="PKY09_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNB13232.1"
                     /translation="MKKEKEFQPSLQEVNASVDTSGKRSLFKKIFAFFGPAYLVSVGY
                     MDPGNWATDIAGGSKFGYQLLWVLLMSNLIALLLQSLSARLGIVRNRDLAQASRETYP
                     HFVNICLYVLAEIAIAACDLAEVLGMAIGLQLLFGISLTTGVMITVLDTFLLLFLINL
                     GIRKMEAFIISLVAIIGFSFLAEIFFAKPNMSEVASGLIPTALSNEALYIAIGIIGAT
                     VMPHNLYLHSSLVQTRKIEKSSKSIWRAIRFNIADSAIALNMAFFVNAAILVLAAAVF
                     HKSGLFEIAEIQDAHKLLAPMLGNNLAPILFAVALIAAGQSSTITGTLAGQIVMEGYI
                     NLRIHPWVRRLVTRLIAIIPALITIHVFGERSTGELLVLSQVILSLQLGFAVIPLIHF
                     VSDKNKMGEFAIGPIIKTLSWVCALIIVGLNIKLVAEEVSVVFHSNTLLIWKVLLGIG
                     CAGTGWLLLMAIFYPLMSKTSFRSKVFHDEPSQISTPESINYKRIAIALDFSSSDKKA
                     IENALRVGGLNTHYLLIHAVESAGALTFGKEIEDMESEHDKKHIEHYIKTLKDMGYFA
                     EFQIAHGNPGKVIPEIVNDFDAGLIVMGQHGHKGLKDFLLGTTIDKVRHKVKCPMLVV
                     "
     gene            complement(11541..12212)
                     /locus_tag="PKY09_10010"
     CDS             complement(11541..12212)
                     /locus_tag="PKY09_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNB13233.1"
                     /translation="MYYQLSPTEQNYIKQIYTLSDGNHQNVLTSDLAAAIKIKAATAT
                     DMLSRLSDKKLVDYEKYYGARLTDKGKQVALNIIRKHRLWEVFLSVKLGFSWDEVHEM
                     AEELEHATNDELSNRLEQFLGNPKTDPHGDPIPNRKGEIEKQKTIRLSETGQDDTVEI
                     RSVEDQNPALLKFLSKHKLTPGARLLVKEINQYDRSLEIKVNNKKVLQLSRDVAENLW
                     IIRVK"
     gene            12328..13359
                     /locus_tag="PKY09_10015"
     CDS             12328..13359
                     /locus_tag="PKY09_10015"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNB13234.1"
                     /translation="MYKLLIKPLLFLFSPEQAHHFVFSMLKMVFKIPGISTLVKSLYA
                     TDSKQKPVLLGNLQFNSPVGLAAGFDKDAYCFDELAAFGFGFIEIGTVTPRPQPGNPK
                     PRLFRLPADEALINRMGFNNEGVDAVVKRLKSRRTDVIIGGNIGKNKDTSNEDALKDY
                     LICFEKLFDVVDYFVINVSSPNTPGLRALQEKEPLTKLLNGIQKKNNSYSNPKPVFLK
                     IAPDLTFEQIDEVAVLVEETKLNGLIVSNTTISRKNLKTNAEAVEKLGAGGLSGKPLR
                     SHSTEIIRYLSQTKKVKFDIIGVGGIHSAADAIEKMEAGAKLVQLYTGFIYEGPGLVK
                     KVNRALIHK"
     gene            13453..14310
                     /locus_tag="PKY09_10020"
     CDS             13453..14310
                     /locus_tag="PKY09_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNB13235.1"
                     /translation="MPEKLKIIECPRDAMQGMHDFVPTDLKIEYINKLLEVGFDTIDF
                     GSFVSPKAIPQMRDTAEVLAGLNKSTTKLLAIIANIRGAEDAVKHQAINYLGYPFSIS
                     ETFQKRNTNATIQESLVNLSTIQQLAQNAGKKLVVYISMGFGNPYGDEWNVDYAAGWI
                     DKIQALGVEIISLADTVGVAKEADIAYLFEGVIQKFPKVEFGAHLHTTPGNWQKKLAA
                     AYDAGCRRFDTAIRGFGGCPMATDKLTGNLATENAVQYLSGKSKNITIDIQKLNDCIL
                     FSKRIFNEH"
     gene            14300..>15448
                     /locus_tag="PKY09_10025"
     CDS             14300..>15448
                     /locus_tag="PKY09_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13236.1"
                     /translation="MSIKKTDSFTKKLFVLAVIFVLTSAPFAFAQKGSLELLGNVKQN
                     GKGLEGAEISIMKGNEQVDNILSGNGGKFIINLDLDAVFTLVFSKAGCVTKSIVFDTR
                     VPEPVKNQLLSNRKFGIELFKVPEGVEQPKELAKPVAKWSWNDAYDDFDYDLEYSNAR
                     KQENEVVKKQLEEMAIVQEKQKKDSLAKARLDSINQAKADEQARLAELAAKKAEEAKQ
                     KKIEEERLAALSAAKKHEEDSLRAAQLKADAIERARLVALEKARQDSIAKANALAEQL
                     AREQAIEQKRLAALEKARQDSIAKADAAAKAQLAALEKARQDSIAKADAAAKAAEAAR
                     LAALEKERKDSLAKAKAEEERLAKEQAKEQARLAALEKARQDSIAKADA"
BASE COUNT         4638 a         3080 c         3106 g         4624 t
ORIGIN      
        1 taaatttaat ccatcagaat ttgaccgtgg cgcatcttca ttaaaatttt ttctatggca
       61 ttgggtcaat gttatttttt tcatcagccc cttttgtcca tcgagtgcta tacgatgttt
      121 tttattgcgc ctgtttggtg caagaatagg gaagggggta gtcatcagca agccgggtgt
      181 taatataaag tttccttggc gcctgaccat tggcaatcat gtgtggatag gcgaaaatgc
      241 atggatttat aatcttgatc aggtaactat aggaaaccat gtaaacattg cacaaggtgc
      301 tatgattcta accggaaatc acaattataa aagctcaaag ttcgaaacca ttttcgggcc
      361 tgttactatt gaagatggtg tttttatagg tgcaaaagct attgtttgcc caaacgtaac
      421 ttgttattcg catagcatac ttgcagcagg ctctgtagct acaacagcac ttaaagccta
      481 tggtatttat cagggcaacc ctgctgaata taaaaaggaa agagtgatac aggagtaaaa
      541 agtaaattag aatagtgtgt tttatgcagt gactgaaaaa gattattgtt ttctaaaatg
      601 ttatcataac aaagattttt tttcttccat aaatttggaa tttcaattaa acaggtttaa
      661 ctttggtttg catttgaata aatgcatgac ataattttaa accaaaaaac catgtttatg
      721 agagctatta cattaccccc cccccgcaaa caattttata aaaaacatct ttagcaggtt
      781 aagaaaaatt gttttgaagc cggaaattat tatcttgcta tttcttttac tgccttgctt
      841 tttacaggca caatacgatg ttgttggcaa taccatactt gtagattggt caagtgccgg
      901 tacatcaacc ctaagtgtaa attacaccaa caccttatta aattgcggag gcactgcaaa
      961 cataactgta gagaccaagc agccttttag cattagtggc cccacaaaaa gttgtccggg
     1021 cgcagatgtt gtgctggcag ctattaatcc tgataacgtg ctgttggatt ggaccctgac
     1081 agatgcatct caaaacacta tatatcttgg tagcggcacc ggtgttcctg attttacaat
     1141 tgcagggtgg acttatccaa gtggcaccta tattattaca gcattagaca ataccaacag
     1201 ttattgtaat tacactgcaa gcatcactat tgaaataacc gattcgccac cgtcaccgcc
     1261 cattccaaca ggcgataacc caatatgtcc gggcacagta catttgtata gcggcacaac
     1321 attgaatgcc ggatattatt tagaatggag tacaacagga ggcactcctg caacaggcac
     1381 aggcaatgaa atatcaatag gctggattaa tacagcgcca ccgcataact attccatcag
     1441 tttggtgcag gtagagtctt ctacaggatg caaatcaatg ccaacggtat tgcctataca
     1501 ggaaagattt tacatcaatc ctgtcatagc aggaaatttt actccctgca ttaataagca
     1561 ggtaacctat cagatttcca atgctgcacc tcttggcaat tatatatacg accaattgga
     1621 atgggtaatt attccttcca ctgccggaag tgtaatcagc gggcaaggca ctactcagat
     1681 aactgttcag tggaataacc agccttcgcc ttccggagtt tacgatacgt atcttcaggt
     1741 aaagacatca ctttgtggtt cgcccccaat cccttatccg aatacggtgt tcgacccacc
     1801 caatatttca cttggcggag caccttcttt ggctattaca gcatcaccca atcctgcttg
     1861 tgaaaataca gctatcacat ttacagccgt accggcacta ccgggtaatt atacctggag
     1921 ttttggtgat ggcaatagcg gcaacagccc attaacagta tcgcacagtt atgcgttgac
     1981 tcaaggtgcc aacctcacaa cctttgatgt taccctgacg gttgatgatg gcggctgcca
     2041 gtcttatgcc agtaccaaag ttgacgttaa tcctgctcca ttatattcat taaccatttc
     2101 tccagtagcg ttctgcccac caactacgac caatataaca gccacgctgg tgcaacaatc
     2161 aggcagtttt aattattcgt ggagtaataa ttcctccaat aataccaaca ctaatacact
     2221 taactactta gacacacaac cattcggtat caccattacc aatacagtaa ccggctgcac
     2281 aacctcacaa accatttcaa tttcaacttg ttcgcccaat cctttgccgt gtaatcccaa
     2341 tccaataaac attagtttta cagcaacacc tgcatcagat tgtcaaacga tagattttac
     2401 agagagcttt aatcccacac cggcaagttt cttatgggat tttggtgatg gctttactag
     2461 tagtcaagcg aatccaacac atacatacag cactgccgga caatatggcg cctgtttgta
     2521 tgcctacgat aataccgggc aacagtattg cgactattat tgtttaccaa tatctttagc
     2581 actttttcct gattttacca cctcattgca ttgcagcagc acgcccggaa gttatgatgt
     2641 ggagtttatt gacctatcag cttttatcaa tccaataacc tcttgggagt ggtattcagg
     2701 caccacactg ctaagcacaa gtaaccaccc aaccattact ttggcagcag gcacctatag
     2761 cattaaacta aaaataggaa acggcaccaa atattgcgaa cgtacactaa caataaatat
     2821 acctgcacca cccgtagcgt catttaccgt tgcaggagga cccttttgcg aggcgcaaac
     2881 ggtagtgcaa cttaatgccg gcaacaccaa cgtgcatcat gccctttggg attttggcga
     2941 tgcttctact tttattccgc aaaacccggc tgcctctatt aatgcgctga aagtgtttgg
     3001 aactcaagga atttattcag ttactttagt agttaccgac aattacggct gcacctacac
     3061 aagcacacaa caggtagcgg ttaacgacaa tcaattagga ggggtaataa gccctgtaac
     3121 tgtttccttt tgcccgggag caagtggaag tttggagtta gatattatta caactccagg
     3181 tccttacaca caacccacct catggttatg gacaggagga attaccacac ccacactaac
     3241 ggtaagtcaa acagggcagt attttgttac cgttagtgat aataacggct gcagctacag
     3301 tcctgccaaa ccgggtcagg cagcagtggt taatgtacca aagcctgtta tttggggcga
     3361 tttgacattg tgcgaaggcg atgccataaa tttagatggc aatcagggta gtgccgtaca
     3421 atataactgg acagtggttg accccaataa tgttactaca acctttagtc aatcgtccat
     3481 tcatattgca ggtgccgttg ccggcaccta tcatgtaaca cttgacatta cagcaaacgg
     3541 ctgcagccgc agcacctctg ttgatgtaga tgtaaattct ttgccgccat tgcccgatat
     3601 tcaaagcagt gtagcacaag cctgcgaagg gcagcccata gacctgacag ttatgaatgc
     3661 cggtacctac tgggtaaact ggagcaatgg cgcaagcgga ccttctgtta cggtttacaa
     3721 tgccggtggc tatcaggcaa cactcacaga tgccaatggc tgcactaacc atgcaacctt
     3781 tgaagtgcat gaaaacccct attcagggtt tatgatgaca ggctgtgtac aatattgcga
     3841 cacgttgcct gtaaccgtat tcggtagtgg aaatttgtca tttgaaaagt ggatatggtt
     3901 tgttgatggt gttgctcaaa cttcggggca gcaaagcccg gtccaaaacc tgaatatggg
     3961 attgctcagt ccgggtgtgt atgtagtaac gctacagttg gtagttgcat atcctgatgg
     4021 gacaaattgc gaaatgatgt ctgatgctct tgaaattacc attataccat gtccttgcag
     4081 gctttatccc gaaggtaaaa tttactgcat gaacgaagta aatggtgatg tacaatcatt
     4141 gaataattat cacttcgaga tagtaaccaa ctatagttgt tctgcaaacc ccaatgttat
     4201 ggtgacttcg ccagatggcg gagtgtcaat gctgccaaac tcaaacccac cgcaactgct
     4261 caccggcata ttatcaacct acagtgccta cccgaacaat gtttgttttg acattaacat
     4321 aacagattct aacaagccgg agtgcagctg caactacctc tattgtatga aattgcccaa
     4381 atgcggagtt cctgtaggct gtggcacaga aattaaaatt gataatgttc aatgtattgg
     4441 tcatgatgcc aacggcaaca gaatttatag ttttacgctt acgctgctaa attcgccaca
     4501 gctattctcc atgtttacgg ctcagggtgg tgttttaggc accatgccgg ccacggttgg
     4561 tcccggcacg actattatca acagttcaat aactgtaaac aataatgcaa gtaccttttg
     4621 catcaccttg catggctaca attttactac ctacacagct tgtaaaacat ctggttgctc
     4681 tggtaatttg cctccttgtg gtactcctcc atcaagaaac ggagagaaaa acactacagt
     4741 gccactgctt cgggtaagct atcaaaatga tggtaacgat gctgttcaga tttcgacact
     4801 aagcctgatg cctaatccgg ctaaaaacag cgttacacta cgttacagtg cccgtacagg
     4861 tggtattatt accatcactt ctgctgttgg acaaaaaatg tataacgaaa aagtaacaac
     4921 acaaggcctg tccgaacttt ctacacaacc atggctgccg ggtatttatt ttgttaccct
     4981 gcaaagcaat gcaggagatt gtttggttca aaaattaatc atcatcaaat aacattcaca
     5041 atgaaaacaa gtgtaaaatt atttcttttc ttcatgatgc tgtttgcaaa tgcaacagca
     5101 caaacccccg gctggcagtg gctgtcgcgt gttggcggct acagcggcaa tggcaatggt
     5161 ggcagtgggc ctgacgaatg ggtaaaagat gtaaagaccg atgcacaggg caacgtgtat
     5221 gtatgtggca gggtatgcta tggagccaac tttaatggcg acaccatgac tacctatcag
     5281 ggctatttta ctttgtttat ggctaagcta aactgtgaag gaaatttagt gtgggtaaga
     5341 acggcaggct cctacggaaa tacagctgat ggagaggctt atggtcttgc attggatgat
     5401 tatggcaata tctatcttac tggatatatt agtgccaacc aattcagtcc tacacagttt
     5461 tttgatacac tgataacaga agaaacatat gattttttct tagccaagtt tgatacctcc
     5521 ggcaattata aatgggcaaa aattgcaggg ccggggccgg gagtaacggg agcaggtgga
     5581 ttcaagattc aaattacaga tgacgatatg attaatgttt ccggtactgg aggtggaggc
     5641 tcttcgggag tttttttccc gggctataat attagtttaa gctggtgctt ttcagcacgt
     5701 tttgatactt ccggaaatat taacaggctt cagaacttag gtagtgtata tggtccaaga
     5761 caaaatgatt acaaaataag cccaacaggc gatcagtaca ttacaggatt tttttattta
     5821 gattctatgg taattggaga tacagtactt tacaaaccgt ataatgcagc ctgccccaca
     5881 tgttgtaata tattcttagt taaatttgat tcgacagggc agtttcagtg ggctcgtaat
     5941 tttggtgata cagtttatag ctttaacaaa ggctatgggt tatctatgtt tgggccagat
     6001 atattattaa caggaggact taatactgct gcaaatattg atggtttctc accaacaaat
     6061 agtttaagta ctaacatata tatggatata ccttttgttg caaagtttaa ctcaaatggt
     6121 gtttgtacgt ggtttacaaa ctcccaacat aaatatggta gttgggcaac aggaggtata
     6181 acaactttga gcaatggagt atcatactac agcggttttt ttggcggacc ggctaagttt
     6241 ggtacggatt cttttgcaag cacaggatta acggatattt ttttagcaga ggtatcacca
     6301 tcaggagtaa ttacatccgg gacaaaaatc acaagcatgg gtgatggtga tgagccacaa
     6361 tgtataacac acgatagcag cggcaacgta tatgttggcg gtagcaacag aagcagttta
     6421 accctaaatg gtgtaaccta cccttttcag ggaggtgaat ccgatggttt tatagccaag
     6481 tggggtacta actgtacggt gggaatagca gaacaggagt ctatgctggc agaagatttg
     6541 tttttatatc ctaacccggc aacaacagaa ataaatattc agaataaagt gcaaggtatt
     6601 accaacataa cagtttatga tgttgtaggc aaaaaaatat tgagcaaaaa aatactgagt
     6661 aaagaactgt tagttaagat agatgtatcg tcattctcac cgggcatgta cattgtaaaa
     6721 gccacaggca atgggtatct gtcaactgcc aagtttgtga gggagtaggg gtgaaatttt
     6781 ttaacatgaa tattaccttg aaaccctttc taatccctct ttggcatgtg gataattatt
     6841 ttgtctcaat tgtaatgctt tggtgtaatc ctctttggca gcagtaatat tgcctacata
     6901 ttcataacac aatccacgat tgtaataggc ttctgcatag ttggggtctg cattaatggc
     6961 atcggtatag tgtttaattg cctggtcata aactttcagg tattcatgat gtatgtatcc
     7021 taagttgaaa tgtgcatttt tgttttttgg gtttatctga ataatttcgg tgtaatactg
     7081 tatggcttta ttatattcct gatgatcctg ataccataat gccaatccgt acatggcttc
     7141 ttcgctgttg gctttaatgt ttaatgcatt ggtgtagtag tccacacaaa gtttgttgtt
     7201 ttgtaaatac atcagtaggc ctaattgtat ttgtgcttca taaaaattgg ggtcaatggc
     7261 aacagcattt ctgaaagagg caatggcagc tgctgtatct tttttctctt ttaagttgaa
     7321 gccctgcatc agcaaagctt ttgtgttttt tgggtctttc tgtaatacag tatctaagta
     7381 aagtaatgac tgctcctgat tgttggtata taaaaatatt tcggctaact taaaagcagc
     7441 atcaaagttt gaaggttgca tttggcgcac cttcactaat gcattgcgtg cgtcttttac
     7501 tttattcatc ctgaagctta ggtcggaata gagcatcaga tattcagggt ctgaaggatt
     7561 gattttaatt gctttttcca tatcgaaata agccaaatca aacatgcttt tttgtgaata
     7621 ggcagtggca cggtcgaaat agttttttgg attggtacta tctgctttga tgttttctgt
     7681 gagctgcgca attttatctt ttgactcttc tgtttctttt ttgccattgg tacatgctgc
     7741 aaatagaagc attgaaaata gaactatgta atgtccgtta aatttcattt ttatgttgtt
     7801 gtggtgcaaa tatcgttata taatgaaaaa agccaccgaa atccggcagc tcttttcttt
     7861 taccaataaa caaataaata ttttactaag cagtttttaa attcattgct tctttaatct
     7921 tattttcaat ctcatcggca agctcaggat tgtcggcaaa aagttgtttc accgcatcgc
     7981 gtccctgtcc gagtttggtg tcgccatagc tgaaccacga accgcttttc tttacaatgt
     8041 ttaattcaac cccaagatca acaagctctc ctgttttgga aataccttcg ccatacataa
     8101 tgtcaaattc tgcctgacgg aatggaggtg ctactttgtt tttcaccact ttaacacggg
     8161 tgcggtttcc tgctacgctg tcaccatctt ttatctgtcc tattctgcga atgtctaaac
     8221 gaactgatgc ataatatttc aaagcattac cacccgttgt tgtttcagga tttccgaaca
     8281 taacaccaat tttttcgcga agctgattga taaaaataca gcaacattta gtgcggctga
     8341 tgcttgcagt aagtttacgt aaagcctgcg acatcaatcg tgcctgcaat cccattttag
     8401 aatcacccat ttcgccttca atttcgctct taggtgttaa tgcagcaaca gagtcaataa
     8461 caatgatgtc aattgcacct gagcgaataa gattgtctgc aatttcaagt gcctgctcgc
     8521 cattgtcagg ctgcgatata agaaggttct caatatcaca acctaatttc tgtgcataaa
     8581 aacgatcgaa tgcatgttct gcatctatat aagcggcaat gccaccattt ttctgtacgt
     8641 tggcaattgc atgaatggct aatgttgttt taccggatga ttcaggaccg taaatttcta
     8701 ttacacgacc tttaggaagt ccgttaatgc ctaatgcaat atctaaggaa atacttcctg
     8761 tctgaatggc ttcaacaggt tcaatggctt cttcgcccat gcgcatgatg gtgcctttac
     8821 cataggtttt gtcaatgcgc tcaatggctg cctgtaaaac ttttaatttc tctttgtcgg
     8881 gtgcttgtac tgtgctcatg tgtcttattt ttttgaatgt ttataacgac aaagtaatgg
     8941 ttttagccaa ttctgattta tgaaaatcca atattttgtt ttcaacagcg gcaatgccgt
     9001 cctcgttgtg attgtaaatc tgtcaattag gcttcatttt tgttttaaaa tcaacaactt
     9061 taatggtcgt aatgcaatca accgggtagt tttaagattt tggctaattt tgtgtaaata
     9121 ttatataatc agcatgttat aataatttta acatatttta tgctgaatgt gattttttat
     9181 tcagattctc agaaacgctt catcacgcgg gcaacttctc tttttgcttc ctgttctttt
     9241 tctgtttgtc gtttatcaaa cattttcttt cctcttgcta aagcaatttc aagtttagcc
     9301 aatccttttt cattaaaata aatacgtgtt ggaattaatg ttgtgccctt ggtttttaat
     9361 ttggtttgaa gtttttctaa ctcccgttta tgcattagta attttctgtc ggacttggca
     9421 atgtgatttg catatccacc ttgtttgtac tcggcaatgt gaatgttttt cacccacagt
     9481 tctccgtttt taaaaacaca ataggcatca gttaaattta cttgtcctgt acgcaaagat
     9541 tttacttcag tacctgtaag tacaataccg gcttcaaact tctgctcgaa aaaataatcg
     9601 aacgaggctt ttttattgac tgtttcggga gcaaaatctt ttttagccat agcggttgca
     9661 aaaataaaat tatactacta acatcggaca ttttactttg tgtcgcactt tatcaatcgt
     9721 agttccaagt aaaaaatctt ttaatccttt atggccatgt tgacccataa caattaagcc
     9781 tgcatcaaaa tcatttacaa tttcaggtat aacttttcca gggttgccat gtgcaatctg
     9841 aaactcggca aagtagccca tatctttcaa agttttaatg taatgttcta tgtgtttttt
     9901 gtcatgttcg ctctccatgt cttctatttc tttgccgaag gtcaatgcac ctgcactttc
     9961 tactgcgtga atcaacaggt agtgcgtatt taatccgcca actcttaatg cattttcaat
    10021 tgccttctta tcacttgagc taaaatccaa tgcaatggca atgcgcttgt agtttatgct
    10081 ttccggagtt gaaatctgac ttggttcatc atgaaatact tttgacctga aagaagtttt
    10141 gctcattaat ggataaaaaa ttgccataag caacaaccat cctgtgccgg cacaaccaat
    10201 gccaagcaga actttccaga taagtaaggt gttgctgtga aatacaacac ttacttcttc
    10261 tgcaacaagt ttaatgttta atcctacaat gattaaagca catacccatg ataaagtttt
    10321 tattatcggg ccaatagcaa attcacccat tttgttttta tcactcacaa aatgaatgag
    10381 aggaataaca gcaaaaccta actgtaaact taaaatcacc tgactaagga caagtaactc
    10441 accggtgctc ctttctccaa aaacatgaat agtgatgagt gccggaataa ttgcgataag
    10501 gcgtgttact aatctgcgca cccagggatg aatacggaga ttaatatatc cttccatcac
    10561 aatctgacct gctaacgttc ctgtgatggt agaactttgc cctgctgcta tcaatgccac
    10621 agcaaaaaga ataggagcaa ggttgttgcc caacatggga gcgagtagct tatgcgcatc
    10681 ctgaatttct gcaatttcaa aaagccctga cttgtgaaac actgctgctg caagtactaa
    10741 tatagctgca ttgacaaaga atgccatgtt caatgcaata gcactgtctg caatattaaa
    10801 tcttatggct cgccagatgg atttgctgct tttttcaatt tttcttgtct gcaccaatga
    10861 cgaatgcaga tacagattgt gaggcataac agtagcgcca attattccaa tagcgatata
    10921 caatgcttca ttactcaatg ctgttggaat taatcctgat gcaacttctg acatgttggg
    10981 cttggcaaaa aatatctcgg ctaaaaatga aaagccgatt attgcaacta aagaaataat
    11041 aaaagcctcc atttttctta tacctaaatt gatgagaaac agaagcagaa aagtatccaa
    11101 tactgttatc attacacctg tggtaagcga aataccaaaa agtaattgaa ggccaatagc
    11161 cattcccaac acctctgcaa ggtcacaggc tgcaatggca atttctgcaa gtacatacag
    11221 acaaatattt acaaaatgcg gataggtttc cctcgatgcc tgtgcaaggt cgcggttgcg
    11281 tacaataccc agacgggcac tcaggctttg caatagtaat gcaataagat tactcattag
    11341 gagcacccaa agcaactgat aaccaaactt gcttccacct gcaatgtctg ttgcccagtt
    11401 accggggtcc atgtagccaa cactaaccaa atatgccggc ccgaagaagg cgaaaatctt
    11461 tttaaacagt gagcgttttc ccgaagtgtc aaccgatgca ttgacctcct gcaacgaggg
    11521 ttgaaattct ttttcctttt tcattttact cttataatcc ataaattttc tgcaacatca
    11581 cgacttagtt gcagcacttt tttattgttg accttaatct ctaatgagcg gtcgtattga
    11641 ttaatttctt ttacaagcaa ccttgcccct ggggtcagct tatgctttga caggaatttc
    11701 aacaaggcag ggttctgatc ttctacagaa cgaatttcaa cagtgtcgtc ttgtccggtt
    11761 tctgaaagac gaatagtttt ttgtttttct atttcgcctt tgcggttagg aatagggtcg
    11821 ccatgagggt cggttttagg attgcccaga aactgttcta aacggttact caattcatca
    11881 ttggtggcat gctctaactc ttctgccatt tcatgaactt catcccagct gaaacctaat
    11941 ttcactgata aaaaaacttc ccacaaacga tgctttctga taatgtttaa tgccacctgc
    12001 tttcctttat cagtcagcct tgcaccataa tatttttcat agtcaaccaa tttcttatct
    12061 gataatcttg atagcatatc cgtagctgtg gcggctttaa tcttgatggc agctgcaagg
    12121 tcagatgtca atacgttttg atgattccca tccgatagtg tataaatctg cttgatgtag
    12181 ttctgctctg ttggtgatag ttgataatac atgttgtcaa aatttagaca aatttagtaa
    12241 taaaatttag attcgtctaa aaatttaaat ttgccatgaa aaaaaacctc ctgactgatt
    12301 ctgttggttt agttttaatt ttgcagcatg tataaacttt taatcaaacc tttgctgttt
    12361 ttattctctc ccgaacaggc acatcatttc gtgtttagta tgttgaagat ggtgtttaaa
    12421 attcccggaa ttagcactct cgtaaaatct ttatatgcta cagatagtaa acagaaaccg
    12481 gttttattag gcaaccttca gtttaattca ccggtagggc ttgcagcagg ctttgataaa
    12541 gatgcttatt gttttgatga gttagcagct tttggttttg gttttattga gataggaaca
    12601 gttacaccac gtccgcagcc gggtaatcct aaaccacgat tattcaggct tcctgctgat
    12661 gaggcactca ttaatagaat gggatttaac aatgaaggag ttgatgctgt tgttaaacgt
    12721 cttaaaagca gaaggactga tgttataatt ggaggaaaca ttggaaaaaa caaagacact
    12781 tccaatgaag atgcgcttaa agattatctt atctgttttg aaaaattgtt tgatgttgtg
    12841 gattattttg tgattaatgt gagttcgcca aacacaccgg gtctgcgggc attgcaggaa
    12901 aaagagccac taacaaaact tttaaacggc attcagaaaa aaaataacag ctatagtaat
    12961 ccaaaacctg tctttttaaa aatagctccc gaccttactt ttgagcaaat agatgaagtt
    13021 gcagttttag ttgaagaaac taagttgaat ggattgattg tcagtaatac tactataagc
    13081 agaaagaact taaaaactaa tgctgaggca gtagaaaagc tgggtgcagg tggcttaagc
    13141 ggaaaacctt tacgaagcca ttcaacagaa ataatccgtt atctttctca gacaaaaaaa
    13201 gtaaaattcg acatcatagg tgtgggtggt attcattcag cagctgacgc cattgaaaaa
    13261 atggaggctg gtgccaagtt agtgcagctt tatacaggtt ttatttatga aggtccggga
    13321 ttagtcaaaa aggtcaatcg tgctttgata cacaaatgac aaaacagtat gttgaatact
    13381 tacgttcatt gctatggaaa tacttcattt acttttttat atttgaaacg tcattaaaac
    13441 caatgaatca ccttgccgga aaagttaaaa ataattgagt gtccgcgtga tgccatgcag
    13501 ggaatgcatg attttgttcc cacggactta aaaattgagt acataaacaa gttgctggaa
    13561 gtaggtttcg ataccattga cttcggtagt tttgtttcgc ctaaagccat tccacaaatg
    13621 cgcgataccg cagaagtgtt ggcaggactt aacaaaagca ccacaaaatt acttgccata
    13681 attgccaata tacgcggtgc tgaagatgca gtaaaacatc aggcaataaa ctatctgggt
    13741 tatccgtttt ctatttccga aacttttcag aaaagaaaca caaatgctac cattcaggaa
    13801 tcgttggtta atttaagtac cattcaacag ttagcacaaa atgcaggtaa aaagctggtt
    13861 gtttacatat ccatgggttt cggcaacccc tatggtgatg aatggaatgt tgactatgca
    13921 gcaggatgga ttgacaaaat tcaggctttg ggagtagaaa tcatttctct tgccgatacg
    13981 gttggtgtgg ctaaagaagc agacattgca tacttgtttg aaggtgtaat ccaaaaattc
    14041 cctaaggtag aattcggagc acatttacat accactccgg gcaactggca aaaaaagctg
    14101 gcagctgctt atgatgcagg ttgcaggcgt tttgatacag caattcgggg ttttggaggc
    14161 tgtccgatgg ctactgacaa attaaccgga aaccttgcaa ccgaaaatgc agtacaatac
    14221 ctttcaggta aatcaaaaaa tataacaata gatattcaaa aacttaatga ttgcatctta
    14281 ttctcaaaac ggatcttcaa tgagcattaa aaaaacagat tcattcacaa aaaaactttt
    14341 tgtgcttgct gtaatattcg tattaacatc cgcccctttt gcttttgccc aaaaaggcag
    14401 cctcgaactt ttaggcaacg ttaaacaaaa cggtaaaggc cttgaaggag ctgaaatcag
    14461 tataatgaaa ggtaatgaac aggtggacaa tattctctcc ggaaatggag gaaagtttat
    14521 tatcaacctc gacctggatg cagtttttac actggtattt tcaaaagcag gatgtgttac
    14581 taagtcaatc gtatttgata cccgcgtacc tgagcctgtt aaaaatcaat tgctatctaa
    14641 ccgaaaattc gggattgaat tatttaaagt acccgaaggt gttgagcaac caaaggagtt
    14701 ggccaagcct gttgccaaat ggagttggaa cgatgcttat gacgattttg attatgactt
    14761 agagtacagc aatgcccgta agcaagaaaa cgaagtggtt aaaaagcaat tggaagaaat
    14821 ggccattgtg caggagaagc agaaaaaaga ttcgttggca aaagcacgtt tggattcaat
    14881 caatcaggct aaggcagatg aacaggcacg actggcagaa ctggcagcaa aaaaggcaga
    14941 agaagccaaa cagaaaaaaa tagaagaaga gagattagcc gctttgagtg ctgctaagaa
    15001 gcatgaggaa gacagtttgc gtgcagcaca actaaaagca gatgcaatag agcgtgcacg
    15061 attagtggca ttagaaaaag cccgtcagga tagtattgca aaagcaaatg cacttgccga
    15121 acagttggca cgtgagcagg caatagagca aaagcggtta gcggcattag aaaaagcgag
    15181 acaagacagt attgctaagg ctgatgcagc agcaaaagct caacttgcag cattagaaaa
    15241 agcccgacaa gatagtattg ccaaagcaga tgcagcagct aaggcagcag aagcggctcg
    15301 attggcagct ttggaaaaag aacgtaaaga ttctttagct aaagctaagg cagaagaaga
    15361 gcgtttggca aaagaacaag ccaaggaaca agcccgttta gcggcattag aaaaggcaag
    15421 acaggatagc atagccaaag cagatgca
//
LOCUS       DAOQNP010000075        14995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1414_length_14995_cov_8.900402, whole genome shotgun sequence.
ACCESSION   DAOQNP010000075 DAOQNP010000000
VERSION     DAOQNP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_14995_cov_8.900402"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKY09_10030"
     CDS             <1..969
                     /locus_tag="PKY09_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13237.1"
                     /translation="PETITPSVIISNSSGVVYISNEGNFQFGNAAISLYDKDSKNIIE
                     DYYKQVNQVPLGDVCQSMVYFNNKFYIVVNNSSKVMVVNAATFKIETTISGFNSPRYF
                     IPVSNSKAYVSDLYDNRLAIVNLSTNIISGYIPAVAGTEQMKMVYGKVFVSSLFTDKI
                     YVINSMTDLLTDSIALAYSPNSICEDKNGMLWIMCSGDSLKQKPSALVQLNPIDNQVI
                     KSLYFNGNHAPWRMCMNKQNDTLYFLDRHVWQMSIGDTTLPTTPFINGNGKLFYALNV
                     DASNGEIYVSDAIDYLQHGSIYRYTASGNLTDQFKAGIIPGGFCFR"
     gene            complement(1000..1073)
                     /locus_tag="PKY09_10035"
     tRNA            complement(1000..1073)
                     /locus_tag="PKY09_10035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1037..1039),aa:Arg,seq:tct)
     gene            complement(1131..1206)
                     /locus_tag="PKY09_10040"
     tRNA            complement(1131..1206)
                     /locus_tag="PKY09_10040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Pro,seq:tgg)
     gene            complement(1298..1386)
                     /locus_tag="PKY09_10045"
     tRNA            complement(1298..1386)
                     /locus_tag="PKY09_10045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1350..1352),aa:Ser,seq:gct)
     gene            1486..3330
                     /locus_tag="PKY09_10050"
     CDS             1486..3330
                     /locus_tag="PKY09_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNB13238.1"
                     /translation="MRVAGINSLFAFLLLMVSCTDHKPQNYTVAETNNSSGFIALADV
                     KTWDPAWSKENKVVYQVLQEPDNLHPTNGKTLMRSEVMLYTQGVLIGTDFKTNTLKPN
                     LLTKLPVYDAVNSTYSFHLRNDVQWDNGEHLNVNDILFTLKVYKSALTQNDFFKPYLD
                     NMLDFIPDSNDSLAFSIKIAKPYLQDVAIWGDVIIMQQSFHDPKNILAKYSLATVAAD
                     NFQSIVPTDVKKWFEEFNSEATGFNLKKLNGLGPYKVTAWEHGQFLTLEKKNNHWTDH
                     SKDSSEHSSVAKIIFKISSDPNVNVLEFKKQQFDASTTLATKSLLKLREDSLFIHNYH
                     AQFTSTFNYSFAAFNMKPDGKKHKKLFDDIKVRKAMSMLFPYEQINRALYKGEYKRQA
                     GPVCSHKYNFNESLTPILQNYSEATKLLADAGWKDSDNDGVLDKMIGGIKEDFKFDMN
                     IYAGIPDWNDLSLMLAAELKKAGIVMTIVPLEVPVFLEKARTHDFDMLMSVWTTNVSP
                     DDFTQLWHTSSWSENGDNYSGFGNNTSDALIDSIKYTVDDSLRTAMDKRFQKMVVDDM
                     AYIFLFNSQRRIAVHKRFGNVNLYFEKPGLLLNTFRLLSPVNKTSAAE"
     gene            3352..4773
                     /locus_tag="PKY09_10055"
     CDS             3352..4773
                     /locus_tag="PKY09_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB13239.1"
                     /translation="MRHIIFKYFFIFSVLLVSSFMLSVYMPGDALDYILMREEKTSIS
                     STHYEHQKTLLAQRLGYDKPLFYFSVKSLAEYGWQQYFPSTQQQKSYNHLLRNYGCGS
                     EVSTFYKKINAARTAIATSFTTATDTSIKHTAALAANCLYQIETQVNLRKNDSILHSF
                     NTYAATLKLTALANSILHSYNEMQTNKSSWKKWVPVIQFNAQNRFHDWFFGTHKNNGI
                     IHLNLGQSLYTGRSIGSLLKEKIFWSLLLTILAILIAFISGIFISMWLTYTRSKLASR
                     IINIITVIFYSLPVYFAGTALLYFFSNPDFLQIFPSSGVMPIGGFSSDVSLTEKFLGT
                     LPYLILPLICYTYSLWAFVQRTSASLISAELKKPYALMAQAKGLTKKEVVKRHIFPNI
                     IAPFVALLANMFPAVIGGSVIIESLFSIPGMGLELIHATTTRNYPFISAVILITGLIT
                     MLLYLLNEWIQHFIDPRTKTVQS"
     gene            4770..5945
                     /locus_tag="PKY09_10060"
     CDS             4770..5945
                     /locus_tag="PKY09_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB13240.1"
                     /translation="MRRKLKFRLAKLWLLAVILLALFAPFLANEKPFFGYDNLTTNDN
                     LPSGFESNGNNQILHPIPYSPGQPGQNDVALLSPFQKNYYTNITGQQIELPLRFHHWL
                     GTDAIGADVAAGIVFGLRHSLIIALLSMIFSIAISLIAGICSGYFADSGIYLNRIKFV
                     TLIIFLFLAWFYCYYTFSLEWYNLFQQFNLINLAGLSTSFMLFLLFTMALVVFWKISN
                     KRKITETKHIHLNPDFWISLLTQVMVAIPQLIIIIALSSLFQPSIITLILVFGLVLWV
                     EPSRMIRAEVQRLRNEGFVDAAIVTGFSFRQIAFRHLIPNLITVVKPIFLYGMAAVIL
                     SESGLSFIGLGVNPGTVTLGGLMAQAKENIDAWWLILFPGLCLFFTILSLSVLSEKK"
     gene            complement(5960..7981)
                     /locus_tag="PKY09_10065"
     CDS             complement(5960..7981)
                     /locus_tag="PKY09_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNB13241.1"
                     /translation="MKKKLLYISNQHEIVLKFIIFCLTAFLIIKMLPHQVRYKFDYSI
                     SKPWTEKELIAPFDFAVLKNKDSLNAEKRILLLNATLFFERDTAVKEQVLQRAIARLD
                     PTDKQEAELLKQEISILANELYKNGVADETARTISFGQAKVIKGVDLIPANNFIFEDD
                     AVNELTRIAKEKHIDAPDKAISILAPLVSNDLTIDKAMTDRFKDELIGSVSETRGMVK
                     KGDLIIAKGEVVTREKYQELESLKSITEEYARPNRHVLIGQVILVVLCLSLLMIFLAQ
                     RRKDIFQSNRRLSIILLMVVFDVFIYTWSLKVEAVSLYIVPLCILPIVLRTFFDTRLA
                     LFGYIITILILGSIASNGFDFVFTQICAGMITIFSIKDMRNRSHVFWAVLFVYIGYFI
                     CYTSLEIIHAGNMVKVNWENSFWLLANVLLTLFAYPLIYFLERIFKVTSDISLIELAD
                     SNNPLLHELSIKAPGTFQHSLQVANLAESAMFKIGGNALLVRVGALYHDIGKMDMPLY
                     FIENQVTQVNPHDELSFDESAGIIISHVIKGIEKARKHNLPDLVIDFIRTHHGTSRVQ
                     YFYESYLKNYPDKIIDENAFHYPGPKPFSRETAVLMMADSVEAASRSLKKHDSESINK
                     LVDDIIDNQIAHGQFSNCNITFRDITELKRLFKKMLGSIYHVRVEYIVT"
     gene            complement(8021..8414)
                     /gene="rnpB"
                     /locus_tag="PKY09_10070"
     ncRNA           complement(8021..8414)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKY09_10070"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8541..9440
                     /gene="xerD"
                     /locus_tag="PKY09_10075"
     CDS             8541..9440
                     /gene="xerD"
                     /locus_tag="PKY09_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNB13242.1"
                     /translation="MSKAAYLNGFSAYIKLEKSLSANSNEAYMHDVELLYEFLLETHP
                     EKNVEKLTLDHLQEFIQWINERNLSARSQARIISGVKAFYKYLLLENIISDDPTTLLE
                     TPKLGRKLPEFLTIEEVDAMLNAIDLSTAEGQRNRAMLETLYSSGLRVSELISLKKSN
                     SFFDIGFLKITGKGNKERLVPIGSSAIKHINIYEESFRRQLKIKTGNDDILFLNRRGA
                     QLTRVMVFTIIKQLAIKIQLKKKISPHTFRHSFATHLIEGGADLRAVQEMLGHESITT
                     TEIYTHLDRDYLRQEIMQHHPRA"
     gene            9437..10684
                     /locus_tag="PKY09_10080"
     CDS             9437..10684
                     /locus_tag="PKY09_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNB13243.1"
                     /translation="MSEKKRLVIVGGGAAGFFSAINAAQLANNLEVIILEKTHKLLSK
                     VRISGGGRCNVTHQCFDNKLLAANYPRGNKELLSCFHQFNAADTIEWFSQHKIKLKAE
                     SDGRMFPDTDSSETIINCFLNEANKSGITIKTGVALTGYSQTEDGQFILHLSDKTTLH
                     ATFLLIASGGHPAASAYSWISNVSNHTILNPTPSLFTFNIPNSPFKGLEGVAVEVTLT
                     LPGYNRTEKGPLLITHWGLSGPVVLRMSAWAAEWLGKKNYLTEVSINFLQGLNHAKAL
                     EIFTENKNTYAKLSLATNKFSTNIPNRLWERLLECANIDKQLQYAQLSKKQINTLVNL
                     LADFRVAMNGKTTFKEEFVTCGGVHLAEINMKTMESKKIYNLFFAGEVINVDGITGGF
                     NFQAAWSTAMIAARNIASRCNNS"
     gene            10695..11243
                     /locus_tag="PKY09_10085"
     CDS             10695..11243
                     /locus_tag="PKY09_10085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB13244.1"
                     /translation="MKNLILFSISIAALLILTSSCKNSPNEQDQMKQQQINPEKLKEQ
                     LVKVNKAQVYSEDDQIEDFLKRYKYPVTTTASGLRYYIYKTNKDIKPNSHSIVEVAYR
                     VHLLDGTVCYSSDSTGNLVFQIGKTDLPVGLQEGVLLMSKGEKAVLITPSKLGYGFTG
                     DGANIPPNAVLYYDVELKKIKK"
     gene            11244..12095
                     /locus_tag="PKY09_10090"
     CDS             11244..12095
                     /locus_tag="PKY09_10090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB13245.1"
                     /translation="MFKQIAFLLSTLLLISSCGKKFNKLESGLEYCIIDAKKNGTNAK
                     PGDILNLNMLYKTENDTVLFNSQAISDSFRLILKKPQYSGDINEGLALMQPGDSAQFK
                     IDAEKFYNESLKQSLPHYIKPGSKIIFEVRLTKITHLKEYEKELSLAKKKKRDEETTL
                     VNNYVIDHNLKGEFLPTGTFFTVIKEGKGRSANAENIVDVLYTGSFLDGTVFDKRTDI
                     QNPFRCKVGSNGLVDGWNEALQRMKEGGKVVVVLPSNSAYGEKGYGPIPPYTPIIFEI
                     ELLKVLK"
     gene            12307..>14995
                     /locus_tag="PKY09_10095"
     CDS             12307..>14995
                     /locus_tag="PKY09_10095"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNB13246.1"
                     /translation="MKHHYFTFILIIFLSINHSLKAQVLPPQFTDQLVSTGWDFLEGY
                     TEDSTGQQYVWEKAGLVWVVDTNGVKLPQPLINISEEVGNWRDHGMNGFAIDPNFRTN
                     GYFYLYYVVDRHYLLHYGTGSYSPTTNEYDAATICRITRYTADAATNFTTTIPGSRLV
                     LLGETKKTGIPVLYDTHSAGTLLFATDGTLIVSAGDGASPATADSGQISSILSYWAQA
                     IADSIITPQQNVGAYRAQLIQSLNGKLLRLDPATGDGIPSNPFYDSTDARSPQSRVWA
                     LGLRNPFRISLKPGTGESDPTAGNPGTFYVGDVGWQFWEDINICNSPGMNFGWPVYQG
                     YDLDPNYFAAKQFNFFAPNPLYGFGGCTQPYFTFHELVKNPTANGIVSFNNSCNPVQP
                     IPATVHTFIHARPVIDYYHGNRARTGIFTGNNASTIDIDNPLSPVDGVRFGGFASVAG
                     PLYQGNKFPLDLQGSYFHADYGGEWIKQFKFNANDTAIYVRDFASNMGPVVFLTENNK
                     DGCLNYVKYPDQIRKICYVGNVNNIPTAVALSDTMYGASPLVVQFNGSQSHDPENDSL
                     SFFWSFGDGFTSTQINPQHTYTSPGGVILSYWAVLTVTDTAGNAAKDSVFISINNTPP
                     VVNITSFNDGDLYSMLQISTLPLQATVTDAEHAPGQLHYKWNVYFHHNQHEHPEPTDT
                     NKITSVTILPAGCDGETYYYRIKLTVTDDGGLSGYNEKSVYPNCTTDCNGVFNGTAFL
                     DSCNTCVGGSTGLQPCTQDCHGDWGGTAFIDSCNTCVGGLTGLQPCTQDCHGDWGGTA
                     FIDSCSTCVGGLTGLQPCIQDCHGDWGGTAFIDSCSTCVGGLTGLQPCTQDCHGDWGG
                     TAFIDSCSTCVGGLTGLQPCTQDCHGDWGGTAFIDSCSTC"
BASE COUNT         4783 a         2835 c         2732 g         4645 t
ORIGIN      
        1 ccagaaacaa taactccatc tgtaattata tcaaacagta gtggtgtggt gtatattagc
       61 aatgaaggaa attttcagtt tggaaatgca gcaatcagcc tgtatgacaa agattcgaaa
      121 aatattattg aagattatta caagcaagta aatcaggttc ccttgggtga tgtttgtcag
      181 agtatggttt actttaataa caagttttac attgtagtca acaattcttc taaagtgatg
      241 gtagttaatg ctgcaacatt caaaattgaa acaactattt cggggtttaa ttcaccacgc
      301 tattttatac ctgtcagcaa tagcaaagct tatgtatcgg atttatacga taacagattg
      361 gcaattgtaa atctgtcaac caacattatc tcaggctata ttccggccgt tgcaggcaca
      421 gaacaaatga aaatggttta tggaaaagtt tttgtttcat cactcttcac tgataaaatt
      481 tatgtcatca attcgatgac agacctgctt acggacagta ttgctttggc ctattcgccc
      541 aactctatct gtgaagataa aaacggtatg ttgtggatta tgtgcagtgg tgattcattg
      601 aaacaaaagc catcggcttt agtacagcta aacccaatag acaatcaggt aataaaaagc
      661 ttatatttta atggcaacca tgcaccctgg cgtatgtgca tgaataagca gaatgataca
      721 ttatattttc ttgatcgtca tgtttggcaa atgagcattg gcgacaccac cttacctaca
      781 acaccgttca tcaatggtaa tggaaaatta ttttacgcac tgaatgttga tgcttccaat
      841 ggagaaattt atgtatcgga tgcaatagac tatttgcaac atggaagtat ctatcgttat
      901 accgcttcag gaaatttgac agatcagttt aaagcaggta ttattcccgg aggattttgt
      961 tttcggtaaa atttaattgg ggatagttaa gaaaagttgt gatcccgatt ggattcgaac
     1021 caatgaccta ctgcttagaa ggcagttgct ctatccagct gagctacggg accaaacagc
     1081 cgtaaacata aaagtctaaa gcggcaacca ctttagactc ttttttcttg tcggggtggc
     1141 aggattcgaa cctgcgacct cctgctccca aagcaggcgc gatgaaccgg gctacgctac
     1201 accccgaaaa atttattaat cggttacgac tcaaagaaca actcttatgc gaagaaacat
     1261 ctacttcgct ctacacctgt tttacgcaaa acttttgcgg tgagggtggg attcgaaccc
     1321 acggtacagg ttaccccgta cgacagttta gcaaactgtt cctttcggcc tctcaggcac
     1381 ctcacctttt cagatgggcg gcaaaggtaa aaaaaagtat atgtaaacaa aactatatgc
     1441 tttaatttta tttttttatg cctcttatat gctaatattg ccattatgag agttgccggt
     1501 atcaacagtt tatttgcatt tttgctttta atggtctctt gcacagacca caaacctcag
     1561 aattatacag tagctgagac aaacaacagt tcaggattta tcgcccttgc agatgtaaaa
     1621 acctgggatc cggcatggag taaagaaaat aaagttgtgt atcaggtact tcaggagcct
     1681 gataatctac accctaccaa tggcaaaact ttgatgcgca gtgaggttat gctttacact
     1741 caaggtgttc tgattggtac agattttaaa acaaatactt taaaacccaa tctccttacc
     1801 aaactaccgg tttatgatgc tgtaaatagt acctatagct tccatttgag aaatgatgtg
     1861 caatgggata atggtgaaca ccttaatgtt aatgatattc ttttcacttt gaaagtttat
     1921 aaaagtgctc ttactcagaa tgattttttc aagccttatc tcgacaacat gttagacttt
     1981 attcctgata gtaatgattc gcttgccttc agtataaaaa ttgcaaaacc ttatctgcag
     2041 gatgttgcca tctggggtga tgtaattatt atgcaacagt catttcatga tcccaaaaac
     2101 attcttgcaa aatattcgct ggcaacagtt gctgctgaca atttccagtc cattgtacca
     2161 acagatgtta aaaagtggtt tgaagaattt aattctgaag caacaggttt taacctgaaa
     2221 aaactgaatg gattagggcc ctataaagtt actgcatggg aacatggaca gtttttaacg
     2281 ttggagaaaa aaaataatca ttggacagat cattccaaag actcttcaga acattcttca
     2341 gtcgcaaaaa taattttcaa aatcagcagc gatccaaatg tgaatgttct tgaatttaaa
     2401 aagcaacaat ttgatgccag cacaacactt gctacaaaat cacttctaaa attacgagag
     2461 gattctttat tcattcataa ttatcatgct cagtttacaa gcactttcaa ctattcattt
     2521 gcggctttta acatgaaacc tgacgggaag aagcataaaa agttgtttga tgatataaaa
     2581 gtaaggaaag caatgtccat gctatttcct tatgagcaaa taaacagagc attgtataaa
     2641 ggagaataca aacgacaagc aggacctgta tgcagtcata aatataattt caatgaatcg
     2701 cttactccaa ttttgcagaa ctacagtgaa gccacaaaac tacttgccga tgcaggatgg
     2761 aaagacagtg ataacgatgg tgtgcttgac aaaatgattg gtggcataaa agaagatttt
     2821 aaatttgata tgaacattta tgccggcatt cctgactgga acgatctttc gctgatgctt
     2881 gctgccgaat taaaaaaagc aggtatagtg atgacaattg ttcctcttga agtacccgta
     2941 tttttagaaa aagcacgaac tcatgatttc gatatgctta tgagtgtgtg gacaacaaat
     3001 gtttcgcctg atgacttcac acaattatgg catacatcaa gttggagtga aaatggtgat
     3061 aactattcag gatttggcaa caacacttca gatgcgttga ttgattcaat aaaatatact
     3121 gttgatgact cactgcgaac tgctatggac aaacgttttc agaaaatggt ggttgatgac
     3181 atggcatata tttttctctt taattcgcag cgcagaattg ccgttcacaa gcgtttcgga
     3241 aatgtaaatc tttattttga aaaacctgga ctgcttctca atacttttcg actactttca
     3301 ccggtcaaca aaacttctgc agcagaataa ataaatttca gaaacaggaa gatgcggcat
     3361 atcattttca aatacttttt tattttttct gtactgttgg tttcatcgtt tatgctgtcc
     3421 gtttatatgc ccggtgatgc attggattac attctcatgc gagaagaaaa aacaagtatt
     3481 tcttcaactc attatgagca tcaaaaaaca ttgctggcgc aacgccttgg ctacgacaaa
     3541 cctttatttt atttttctgt aaagtcctta gctgaatatg gatggcaaca atattttcct
     3601 tcaacacaac aacaaaaaag ttacaatcac ttgcttcgca attacggttg tggcagtgaa
     3661 gtatctactt tttataagaa gataaatgcc gcaagaacag caattgctac atcattcacc
     3721 acggcaaccg atacttcaat aaaacataca gcagctttag cagcaaactg tttatatcag
     3781 atagaaacac aagtaaatct gagaaagaat gacagcatcc ttcattcctt caacacctac
     3841 gctgcaacac ttaaacttac tgcactggct aattctattt tgcattccta taatgaaatg
     3901 caaactaata aatcatcatg gaagaaatgg gttcctgtaa ttcaatttaa tgcacaaaat
     3961 cgttttcatg actggttttt tggaacacat aaaaataatg gcatcataca tttaaactta
     4021 ggccaatcgc tttataccgg aagaagcatt ggctcattgt tgaaagaaaa aatattctgg
     4081 tcactgttgc tgactattct ggcaattctt atagcattta tttccggcat atttatttca
     4141 atgtggttga cctatacaag aagcaaactt gcaagccgca tcatcaatat aattacagtg
     4201 attttttatt ctttaccggt ttattttgca ggcacagctt tactatattt tttctctaac
     4261 cctgatttct tacagatatt cccatcttcg ggtgtaatgc caattggggg attttcttca
     4321 gacgtaagtt tgacagaaaa atttttgggt acactaccct accttattct gccattgatt
     4381 tgctacactt attcattgtg ggcatttgtt caacgtacat ctgcaagcct catatcggct
     4441 gagttaaaaa aaccttatgc cctaatggca caggcaaaag ggctaacaaa aaaagaagtt
     4501 gttaaaagac acattttccc aaatattata gcaccctttg tagccttgct tgccaatatg
     4561 tttccggctg ttattggtgg ttcggtaatt attgaatcac tattttctat tccgggtatg
     4621 ggacttgaac tgattcatgc tacaacaaca agaaattacc ccttcatttc tgctgtgatt
     4681 ttaattacag gactcattac gatgttgctt tatctgctga acgaatggat tcagcatttt
     4741 attgatccac gaaccaaaac tgttcagtca tgaggagaaa gttgaaattt cgtttagcaa
     4801 aactttggct tctggcagta atactccttg cattgtttgc tccgtttctt gccaatgaga
     4861 aaccattttt tggatatgat aatctaacta ctaacgataa tttaccatca ggatttgaga
     4921 gcaatggaaa caatcagatt ctgcacccta taccctactc accgggtcag ccaggacaaa
     4981 atgatgtggc tttattaagc ccgtttcaaa aaaactatta cacaaacatt accggacaac
     5041 aaattgaatt gccactacgg tttcatcatt ggttaggcac agatgcaatt ggtgctgatg
     5101 tagctgcagg aattgttttt ggtttacgac acagtttaat tattgctttg ctttcaatga
     5161 ttttcagtat tgccatttca ctgattgcag gtatctgttc aggatatttt gctgactccg
     5221 gaatatattt gaatagaata aaatttgtta cactaattat atttttattt ctggcctggt
     5281 tctattgtta ttacacattc tctttagaat ggtataacct ctttcagcag tttaacttaa
     5341 tcaatttagc aggcttatca acttccttta tgctattcct gctttttaca atggctttag
     5401 tagtattttg gaagatttcc aataaaagaa aaatcacaga gacaaaacac atacatctca
     5461 atcctgattt ttggatttcg ctgctgacac aggtaatggt agccatacca caactcatta
     5521 tcatcattgc cctctcttca ttgtttcaac catctatcat cacattaata ttagttttcg
     5581 gattggtact ctgggttgaa ccatcaagaa tgattagggc agaagtacag cgactgcgta
     5641 atgaaggctt tgttgatgct gcaattgtta ccggattttc attcagacaa attgcattca
     5701 gacatttaat acccaattta attactgttg taaaacccat attcttatat ggaatggcag
     5761 cagtaatact gagtgaatca ggtctttcgt ttattggctt aggcgtaaat cccggaaccg
     5821 ttaccttagg tgggctgatg gcgcaggcaa aagaaaatat tgatgcctgg tggctgatac
     5881 tctttccggg cttatgttta ttctttacaa ttctgagttt gtcagtcttg agcgaaaaaa
     5941 aataaatctt aatattttat cacgtcacta tgtattctac tcgcacgtga tagatactgc
     6001 caagcatttt cttaaacagc cttttcaatt cagtaatatc tctgaatgta atattacagt
     6061 ttgaaaactg tccatgcgca atctgattat caataatatc atcaaccaat ttattgatgc
     6121 tctcagaatc gtgcttctta agacttcgtg atgcagcttc aacagaatct gccatcatca
     6181 gcacagcagt ctctctgctg aaaggtttcg ggccgggata atgaaatgca ttttcatcaa
     6241 taatcttgtc aggataattt ttcagatacg actcgtaaaa atactgtaca cgtgatgtac
     6301 cgtggtgtgt tcttataaag tcaataacca aatcgggtaa attatgcttt ctggcttttt
     6361 ctattccttt aatcacatgg ctgataatga tacctgcact ttcatcaaaa gacaactcat
     6421 catgtggatt tacctgcgtt acctggtttt caataaaata aagtggcata tccattttac
     6481 cgatgtcatg atacaatgca cctactctga ccaacaaggc attgcctccg attttaaaca
     6541 ttgctgactc ggcaagattc gcaacctgca atgaatgctg aaatgttccg ggtgctttta
     6601 tactcagttc atgtaacaat ggattgttgc tatcagcaag ttctatcaat gaaatatcag
     6661 aagtaacttt aaaaatgcgc tcaaggaaat atattaatgg ataagcaaaa agtgtcagca
     6721 gaacgttagc aagcaaccag aaagaatttt cccaatttac tttaaccata tttcctgcgt
     6781 gaataatttc gaggctggta taacaaataa aataaccgat gtaaacaaac aatacagccc
     6841 agaaaacatg cgagcggtta cgcatatcct taatactgaa aatagttatc atgcccgcac
     6901 aaatttgtgt aaacacaaaa tcaaaaccat tggatgcgat tgatcctaaa attaatattg
     6961 tgataatgta gccaaacaag gcaagtcttg tatcgaaaaa agttcgcagt acaatgggga
     7021 gaatacatag cggaacaata tataagctca ccgcttcaac tttaagcgac catgtgtata
     7081 taaagacatc aaaaaccacc atcaataaaa taatacttag tcttctgttt gactgaaaaa
     7141 tatcttttct gcgctgtgcc agaaatatca tcaataacga aagacataaa acaaccaata
     7201 ttacctgtcc gattagaaca tgtctattgg gtcgtgcata ctcttcagtg attgatttca
     7261 aagattccaa ctcctgatat ttttcacgtg tcactacttc acctttggca ataatcaaat
     7321 cgcctttctt aaccattccg cgcgtttcag agaccgaccc aatcaactca tctttaaaac
     7381 ggtcagtcat tgctttgtca atagttaaat cgttactcac caaaggagca agtattgaga
     7441 ttgccttatc aggagcatca atatgttttt ccttagcaat acgggtcaac tcattaacgg
     7501 catcatcttc aaaaataaaa ttgttggcag gtatcaggtc aacacccttg attactttgg
     7561 cctgaccaaa ggagatggtt cgtgcagttt cgtcagctac accattctta taaagttcat
     7621 tagcaagaat tgaaatctcc tgcttcaata actctgcttc ttgtttatct gttggatcaa
     7681 gacgtgctat tgcacgttgt aaaacctgct ccttaacagc agtgtcacgt tcaaaaaaaa
     7741 gtgttgcgtt gagaagtaga attcttttct ctgcattgag cgaatcttta tttttcaaaa
     7801 cagcaaaatc aaacggagca atcagttctt tctcagtcca gggcttagat atagaataat
     7861 caaacttata ccttacctga tgcggcaaca ttttaataat cagaaaggct gtaagacaaa
     7921 agataataaa tttaagaact atctcatgct gattgctgat gtaaaggagc ttctttttca
     7981 ttggctgcta aattattaaa aagcaatgca gcagaaaaaa ataaaaagca agcctgtaag
     8041 ccggattctg ttatctgaat actttgcagc aatcaaatcc cctatcattt gtctgtttgc
     8101 ggcataccct tcacgaagat gttcattgct gaacaaacgg caacgagcca ttacctgttc
     8161 gtgatttatt ttgcctttca actcctgagg tttaccttgc attttcattg ctgaatatgc
     8221 ccgtaagctc ttacctcacg ttttcacctt tacctccgtg caatacattg ccggtggaat
     8281 tattttctct gtggcacttt ctgttatagc ttcatcgctt tagctacacc ctcctttaac
     8341 aggaggcagg atgctctgtg ttgcccggac tttcctctct taaacgagtt aagagcgata
     8401 gagcggcttg ctgatacaaa aatacagttt attaccttta gtttttagtt aatgtcaata
     8461 aacaatcaat cgttgatttt ttaagttaat taaatcaaaa cacactcatg aaatgaattt
     8521 cggttagcta tcttagcaaa atgagtaaag cggcttatct taatggtttt tcggcataca
     8581 ttaaactgga aaaatcatta tctgccaatt ccaatgaagc ttatatgcat gatgtggaat
     8641 tgctttatga atttcttctg gaaacccacc cggaaaagaa tgtagaaaag ctcacattag
     8701 accatcttca ggaatttatt cagtggatta acgagcggaa tctcagtgcc cgttcacaag
     8761 ccagaattat ttccggtgtt aaagcattct ataaatatct cttacttgaa aatattatct
     8821 ctgacgaccc aacaacgcta ttagaaacac ctaaacttgg cagaaaactt cctgaatttt
     8881 taactattga agaagtggat gctatgttaa atgctattga tttgagtaca gcagaaggtc
     8941 agcgtaacag agctatgctc gaaactcttt atagttcagg attacgtgtc agtgaactta
     9001 tttccttaaa aaaatcgaat tcattcttcg acattggatt tttaaagatt accggtaaag
     9061 gtaataaaga aagactggta ccaatcggct cttctgcaat taaacatatt aatatttacg
     9121 aagagtcttt cagaagacaa cttaaaatca agacaggtaa tgatgacatt ctttttctga
     9181 acagaagagg tgcacagtta acacgtgtga tggtttttac aatcatcaaa caattagcaa
     9241 ttaaaattca gcttaagaaa aaaatcagtc cgcatacgtt cagacatagc tttgccacgc
     9301 acctgattga aggtggtgcc gatttacgtg ctgtgcagga aatgctcggc catgaaagta
     9361 ttaccacaac agaaatttac actcacttag acagagatta tctccgtcag gaaattatgc
     9421 aacatcatcc acgcgcatga gtgaaaagaa acgacttgtt attgttggtg gtggtgctgc
     9481 cggttttttt tctgctatca atgctgcaca actggcaaac aatctggagg taattatctt
     9541 agaaaaaaca cataaacttt tatcaaaggt tcgtatttcc ggtggtggcc ggtgcaacgt
     9601 gacgcatcaa tgttttgata ataaattatt ggctgcaaat tatcctcggg gaaataaaga
     9661 attgctaagt tgctttcatc aattcaatgc tgctgacact attgagtggt tcagtcagca
     9721 caaaataaaa ttaaaagcag aatctgacgg aagaatgttt cctgacactg attcgtcaga
     9781 aaccataatc aattgctttc tgaatgaagc aaacaaatcc ggaataacta ttaaaaccgg
     9841 ggttgcactg actggttatt ctcaaacaga agatggtcaa tttattttgc atctctctga
     9901 caaaactact ttgcatgcca catttttact cattgcttca ggcgggcatc ctgcagcatc
     9961 tgcatattcg tggatttcga atgtatccaa tcatacgata ttaaatccaa cgccctcact
    10021 ttttactttc aatatcccaa attcaccatt caaaggtctt gaaggcgttg ccgtagaagt
    10081 tactttaacc ttacccggct acaacagaac tgaaaaaggc ccgttactca tcacacactg
    10141 gggattgagc ggtccggtag ttttaagaat gtctgcatgg gcggcagaat ggttaggcaa
    10201 gaagaattat cttactgagg tcagcataaa ttttttacaa ggattaaatc atgctaaagc
    10261 attggagatt tttactgaaa ataaaaacac ttatgccaag ctaagtttag caaccaataa
    10321 attttccacc aatataccga accgattatg ggagcgttta ctcgaatgtg caaacattga
    10381 taaacaacta caatatgcgc aactatcaaa aaagcaaatc aacaccttag taaatttgtt
    10441 agctgacttt cgagtagcga tgaacggaaa aacaacattc aaagaagaat ttgtgacgtg
    10501 tggtggcgtt cacttggctg aaatcaatat gaaaactatg gaaagcaaaa aaatctacaa
    10561 tctgtttttt gcaggagaag tgattaatgt tgatggcatt accggtggat ttaactttca
    10621 ggctgcatgg tcaacagcaa tgatagcagc tcgcaatatt gcgtcaagat gtaacaatag
    10681 ctaactttgc gaagatgaaa aatttgattt tattttctat ttcaattgct gctttgctga
    10741 ttcttacatc ttcttgtaaa aatagcccta acgaacaaga ccaaatgaag caacaacaaa
    10801 taaaccctga aaaattaaaa gaacagttag taaaagtcaa caaagcgcag gtttattcag
    10861 aagatgatca gattgaagat tttcttaagc gatataaata tccggttact actactgcat
    10921 ctggattgag atactatata tataagacta ataaagatat taaaccaaac tcccacagca
    10981 ttgtcgaagt tgcttacaga gtccacttgc ttgatggcac tgtttgttat tcatctgaca
    11041 gtaccggtaa tttggttttt caaatcggca aaactgacct tcctgttggt ttacaagaag
    11101 gagttctact gatgtcaaaa ggagaaaagg ctgttttaat aacaccttca aaattgggtt
    11161 atggtttcac cggtgatggg gcaaacattc cacccaatgc agttctatat tacgatgtcg
    11221 aattgaaaaa aataaaaaaa taaatgttta aacaaattgc ttttctttta tctacactac
    11281 ttctgatttc atcctgtggt aaaaaattca acaaacttga atccggtctt gaatactgca
    11341 ttattgatgc aaagaaaaat ggaacaaatg caaaaccggg agatattctc aatttaaaca
    11401 tgctatataa aactgagaat gacactgtgc tgtttaattc gcaagccatt tctgattcat
    11461 tcagacttat tctgaaaaaa ccacaatatt caggcgatat taacgaagga cttgctttga
    11521 tgcaacctgg tgatagtgca caatttaaaa ttgatgcgga aaaattttat aatgaatcac
    11581 tcaaacaatc tcttccgcat tatattaaac cggggagtaa aataattttt gaagtacgat
    11641 tgactaaaat tacgcatctc aaggagtatg aaaaagagct ttcattagca aaaaagaaaa
    11701 agagagatga agagacaacg ttagtaaaca actatgtaat tgatcataac ctgaaaggag
    11761 aatttcttcc aacaggaact tttttcacag taattaaaga gggaaaaggc cgcagtgcca
    11821 atgctgaaaa tattgttgat gttctgtaca caggcagttt tcttgatgga actgtttttg
    11881 acaaacgcac agatattcag aatccttttc gctgtaaagt tggaagcaat ggacttgttg
    11941 atggatggaa tgaagcttta caacgaatga aagaaggtgg caaagttgta gttgtacttc
    12001 cttctaacag tgcttatggc gaaaaaggtt atggtcctat tccaccttac acaccaatta
    12061 tttttgaaat tgagttgtta aaagttctga aataaatatc agactttttt aatagtgtca
    12121 ttataaaaaa aaagattgaa tttacttcaa ttgcatactt gttaaaagca aacatatcat
    12181 tcactaataa caaattaaca ctttttatac tcttaaactg taagttgaaa actaaaaagg
    12241 ttttctatca ctaatacttt ttagacattt atatttgttt ctgcaattct gtaataaacc
    12301 gatatcatga aacatcacta ctttactttt attttaatta tttttctttc aataaatcat
    12361 tcactaaaag cacaggtact tccaccacaa tttactgatc aacttgtaag cacaggttgg
    12421 gactttttag aagggtacac cgaagattca acaggacaac aatatgtttg ggaaaaagcc
    12481 ggattggttt gggtagttga tactaatggc gttaaattac cacaaccttt aataaacatt
    12541 agtgaagaag tgggcaattg gcgtgatcat ggcatgaatg gttttgcaat tgaccctaac
    12601 ttcagaacta acggatattt ttatttatat tatgttgttg ataggcacta tcttcttcac
    12661 tacggtacag gcagctacag tccaacaaca aatgaatatg acgctgcaac tatttgccgc
    12721 attacaagat atacagcaga tgctgcaact aactttacaa caactattcc cggaagcaga
    12781 cttgttttac tgggtgaaac aaaaaaaaca ggaatccctg ttttgtatga cacacatagt
    12841 gcaggaacat tattatttgc aacagatgga acattgattg tatctgccgg tgatggtgct
    12901 tcacctgcaa cagcggatag tgggcaaata tcttctattc tcagttactg ggctcaagca
    12961 atagccgata gtattatcac accacaacaa aatgtgggtg cttatcgggc acagctaatc
    13021 caatcattga atggaaaact tttgcgatta gatccggcaa ccggtgatgg aattccttct
    13081 aatccttttt atgacagtac cgatgcacgt tctcctcaat caagagtttg ggcattggga
    13141 ttaagaaatc catttcgcat ttcacttaaa ccgggtacag gagaatcaga ccccacagca
    13201 ggtaaccccg gaacatttta tgtaggtgat gtgggttggc aattttggga agacataaat
    13261 atatgcaata gccccggaat gaattttggc tggcctgtat atcaaggtta tgatttagat
    13321 ccgaactatt ttgctgcaaa acaatttaac ttctttgcgc ctaatccact ttatggtttt
    13381 ggtggttgca cacaacccta ttttacattt catgaattgg taaaaaatcc tactgctaat
    13441 ggcattgtaa gttttaacaa ctcttgtaac ccagtacaac caattccagc aaccgtgcat
    13501 accttcattc acgcacgacc tgttattgat tactatcatg gcaatcgcgc ccgaaccgga
    13561 attttcaccg gaaacaatgc ttcaacaata gatatagata atccattatc gcctgtagat
    13621 ggtgttcgtt ttggcggatt tgcctctgtt gcaggaccat tatatcaggg caataaattt
    13681 cctttggatt tacaaggaag ttatttccat gctgattatg gtggtgaatg gattaagcaa
    13741 tttaaattta atgcaaacga tacagcaatc tatgtaagag attttgcatc caatatggga
    13801 cctgtagttt ttctgactga aaataataag gatggttgtc tcaattatgt caaatatccc
    13861 gatcaaatca gaaaaatatg ttatgttgga aatgtaaaca acatcccaac tgctgttgca
    13921 ttgtctgaca ccatgtatgg tgcatcaccg ttagttgttc agtttaatgg aagtcaatca
    13981 catgatccgg agaatgattc actatctttc ttctggagtt ttggcgatgg ctttacttct
    14041 actcagatta acccacagca tacttacaca agcccgggtg gtgtaattct aagttattgg
    14101 gctgtattaa ctgttactga tacagcaggt aacgctgcca aagactctgt ttttatctca
    14161 ataaataata cacctcctgt tgtaaacatt acaagtttta atgatggtga tttatattcc
    14221 atgcttcaaa tctcaacgct accacttcag gcaacagtga cagatgctga gcatgcaccg
    14281 ggtcaactac actataaatg gaatgtttat tttcatcata accaacatga acatccggaa
    14341 cctactgaca caaacaaaat tacttctgta actattttgc ctgccggttg cgatggtgaa
    14401 acttattatt accgaataaa acttacagta actgatgacg gaggcctcag tggttacaat
    14461 gaaaaaagtg tttatccaaa ttgtacaacc gattgtaatg gtgtttttaa tggcactgca
    14521 tttttagatt cttgcaatac ttgtgttggt ggtagcaccg gactgcaacc ttgcacacag
    14581 gattgtcacg gtgattgggg tggtacagca ttcattgatt catgtaatac atgtgttggt
    14641 ggattaacag ggctacaacc ctgcacacaa gattgtcatg gcgactgggg tggtactgca
    14701 tttattgatt cttgcagcac ttgtgtaggt ggactaacag gattacaacc atgcatacaa
    14761 gattgtcatg gtgattgggg cggtactgca tttattgatt cttgcagcac ttgtgtaggc
    14821 ggactaacag gactgcaacc ttgcacacag gattgtcatg gtgattgggg cggtactgca
    14881 tttattgatt cttgcagcac ttgtgtaggc ggactaacag gactgcaacc ttgcacacag
    14941 gattgtcatg gtgattgggg cggtactgca tttattgatt cttgcagcac ttgtg
//
LOCUS       DAOQNP010000076        14353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1499_length_14353_cov_9.215345, whole genome shotgun sequence.
ACCESSION   DAOQNP010000076 DAOQNP010000000
VERSION     DAOQNP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_14353_cov_9.215345"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKY09_10100"
     CDS             complement(<1..328)
                     /locus_tag="PKY09_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13247.1"
                     /translation="MQLFNFVISAAIVSVVLFTSCKKKDDDNASPNPPAQSAGFYYGE
                     NGITTLTKADSSWVNGAYNTIIAQSSGSTVVEINLTGITSQGYSLGTTNVLTYLKSGS
                     SWVATTG"
     gene            complement(472..858)
                     /locus_tag="PKY09_10105"
     CDS             complement(472..858)
                     /locus_tag="PKY09_10105"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HNB13248.1"
                     /translation="MNPSPMRAALKLKCPKCAQSNLFTDPNPYHFKKLGDMPVVCPNC
                     ALPFTPEPGFYYGAMYVSYALTVAMSVFNFIWVYLLWGFVVAPYLIINSVLLIALFPL
                     FFRYSRSLFLAMVFKIEEAARKRQRH"
     gene            complement(858..1580)
                     /locus_tag="PKY09_10110"
     CDS             complement(858..1580)
                     /locus_tag="PKY09_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNB13249.1"
                     /translation="MEQIRFKQPDIGSQVLFEDNHLIAINKLPGQITQGDKTGDVPLG
                     DWVKQYLKEKYQKPGNVFCGTIHRLDRPVSGVVLFAKTGKALERMNEQFRERKVQKTY
                     WAVVKNKPAKEEDTLVHYLVKNEAKNKSFASLQEKNGTTRCELSYKLLASSDHYHLLE
                     VNPLTGRHHQIRVQLSAIGCPIKGDLKYGADRSNADGSIHLHARKLTFVHPVKSEPVC
                     IEAKPPEEPLWNYFVQQLTNTK"
     gene            complement(1580..2395)
                     /gene="panB"
                     /locus_tag="PKY09_10115"
     CDS             complement(1580..2395)
                     /gene="panB"
                     /locus_tag="PKY09_10115"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNB13250.1"
                     /translation="MSVHSEVKKITTHVLQEMKRSGEKISMLTAYDFSLAGIIDDAGI
                     DVILVGDSASNVMAGHETTLPITLDQMIYHASSVVRAVKRALVVVDLPFGSYQGNSKE
                     ALQSAIRIMKESGAHAVKLEGGREVKESIERILTAGVPVMGHLGLTPQSIYKFGTYVV
                     RAREEEEAKRLIEDAHILEAAGCFAIVLEKIPAALAAIVSQEVKIPVIGIGAGNGVDG
                     QVLVLHDMLGINNEFHPRFLRRYLNLYDDIKAATAKYITDVKSRDFPNSKEQY"
     gene            complement(2406..3569)
                     /locus_tag="PKY09_10120"
     CDS             complement(2406..3569)
                     /locus_tag="PKY09_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13251.1"
                     /translation="MYKLLFLLMFTAVALSKLTPVQAQTVNEKFDRLDLSENFDTSSG
                     IWTFMSNSENLFLVQEGEYILNRKAPEKPYAIIANYDYNQPAFKMITSLKIEKANDQN
                     GSVGVMFMAQNDGNGGFIFEINTLKQFRLRQIVAGSYRYLTGDAKSGGWVKSNFINNL
                     NVYNLLELRFSQRNYDIYLNNNLLMSFTEIAYKSGRFGYIVGPGSRAKIDFLYLFSAS
                     GKSESTAGSSNNNNQQEGSPDVIQLAESIIKLKTQINKLQAENEELTRNLESVKSADQ
                     EDENERKLKDKIITDLQKQMTIKEASFDSLLKVNQTLLKYKEMVAGNENADLIITLSK
                     SVKAEKEKNKQLSDSLQILKTELMKQKGTKAAPPAEKKEPQKVNTEFTLPKEN"
     gene            3667..4662
                     /locus_tag="PKY09_10125"
     CDS             3667..4662
                     /locus_tag="PKY09_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13252.1"
                     /translation="MNKLLISFCLLCLSLSSCKTDVDLLAPYKEVMVIYGVLDPTDSV
                     QYIRINKAFLGEGNAYTFAQVADSFQYADVLDVKLERYKNNNFAGAINLERFEGPPLK
                     DGIFASAPNYLYRTTGLDSIYQDSEYKLVVTNTQTGNVVTSKTAVVSKAGSIINPNPS
                     SLYGYVNFADPANPFVTKFSSSDKAKYYDLNIKFNYKEIEIANPSNFEMKSIFFRVDG
                     VEKQSNNSLEIPVSYTTFYSALRRLIPVNPFVKREAEPLDFVLVGGAEELYTYYQVNR
                     PPTGINQSIPQYTNIEGGIGIFSSKYTDIVSHKSLSQTSMDSLINGAITKDLGFQ"
     gene            4794..6716
                     /gene="dnaK"
                     /locus_tag="PKY09_10130"
     CDS             4794..6716
                     /gene="dnaK"
                     /locus_tag="PKY09_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNB13253.1"
                     /translation="MRKVIGIDLGTTNSCVAVMEGNEPVVIPNSEGRRTTPSIVGFIK
                     DGERKVGDPAKRQAITNPKNTVYSIKRFMGETYDHASKEKDRVPYEVVKGDNNTPRIK
                     INDRQYTPQEISAIILQKMKKTAEDYLGNEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVLRIINEPTAAALAYGLDKKNKDQKIVVFDCGGGTHDVSVLELGDGVFEVKSTDGD
                     THLGGDDFDQRIIDWLADEFKSDEGIDLRKDPMALQRLKEAAEKAKIELSSSTQAEIN
                     LPYIMPVDGVPKHLVKTITRAKFEQLVDDLIKRTIEPCKTALENAGLKPSDIDEVILV
                     GGSTRIPGVQSAVEKFFGKSPSKGVNPDEVVAIGAAIQGAVLTGEVKDVLLLDVTPLS
                     LGIETFGGVFTKLIEANTTIPTKKSEVFSTASDNQPSVEIHVLQGERSMAKDNRTIGR
                     FHLDGIPPAPRGVPQIEVIFDIDANGILHVTAKDKGTGKEQKIRIEASSGLSDDEIKR
                     MKTEAEANAEADKKAKEEADKVNMADTMIFQTEKQLTEYGDKLSQSNKENIQRALSKL
                     KDAHKEKNLADIDTALNELNTAWQGASQEMYNATNNTANTTAGGADANINAETQNNTA
                     GEVTDVDFEEVKDDKK"
     gene            7135..8433
                     /locus_tag="PKY09_10135"
     CDS             7135..8433
                     /locus_tag="PKY09_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNB13254.1"
                     /translation="MKFEKPPVDETFDIKIVKLIIDEQYKDFLQTVESRYLYWDEVKN
                     RKDLPFDPAKSWRLIKTSRALNSKSVNIGAYVFKYYLTYQIQKDLHEFDLKMIGGLYQ
                     NPITDFEKAEYIKNSLMEEAIASSQIEGAATTTKIAWDMLKSGRNPRNESEQMIFNNL
                     RAIRYIEENQNKNIDKAFIIELHKIMTVKTTAEDCGGDYRKGEIFVQDHVDGEIAHIP
                     PDWNELGSLMDSLCSFANSDKEFMHPIIKASMIHFFIGYIHPFKDGNGRTARALFYWY
                     LLKKGYTLIKNISISRAILSSRIQYDKAFLKTEYDQNDLNYFITYSIKSLRVAFENLV
                     RYRDKKKADKEQASVIAYRLIEKGMSKRQADLLGYLYSKESAAVSLQQYAIKHDIVRQ
                     TARKDLSHLVKLNLLIEEKKGKSIVFRVKSKSDVEKFLDK"
     gene            complement(8719..8803)
                     /locus_tag="PKY09_10140"
     tRNA            complement(8719..8803)
                     /locus_tag="PKY09_10140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8767..8769),aa:Ser,seq:tga)
     gene            8909..10360
                     /locus_tag="PKY09_10145"
     CDS             8909..10360
                     /locus_tag="PKY09_10145"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB13255.1"
                     /translation="MKRLLLFLALGSIAALTQAKTIKALFDYKSFYAPGQGSYLEWYL
                     SVKGNSVNYKMSESGNYEAAVAVAYKITQNDKIVAQDSYNLISPLTKDTLLVSDFIDN
                     KRLQLPNGKYNIELSVKDNVADSKTFTINQGFELAFEPGKTSVSDIELVQHYSKKETG
                     NNTLVKNGFEIIPRVDNFYQTNEERLIYYAEAYFTNSLTDDAVAVYSYLQNAESGSTI
                     MDYGTVQRKKTSEVIPLLSEINIANLGSGNYNLIVEVKDKNNQSLALSKIFFQRQSVV
                     KDNFVLNNDVSSTFVATMNDKNVLAENIACLQPISNAQQSEWIGNQLKAANIDMMKNF
                     FYDFWQRREPVDPQKAWNDYKAHVDAVQQKFGSRALKGYNTDMGRVYLAYGAPSTINK
                     GEYEPNTYPYEIWRYDKINNHTNKTFVFYSKDLLARNFTLLHSDMPGEPYEAAWNMIL
                     HDRSIKAKNIDDTGKDLKGVYSGDRTNDNFNAK"
     gene            10368..11219
                     /locus_tag="PKY09_10150"
     CDS             10368..11219
                     /locus_tag="PKY09_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB13256.1"
                     /translation="MAFLFLKFLIRCIAFLDLRILYLLADVIAFCFKYIFPYRKKVIL
                     QNLYNSFPEKNEQEIEEIAKQFYNYFADLIVETIKMSSISKEELLRRCRFTNPEILHN
                     YYEKKRSIIAVCGHFNNWELGALALSVIDKHQMLGVYKPLSNNSWNEYFKTIRSRFGM
                     IPVPMKSILRELIKRKNEPTITALAADQTPHRSEINYRTTFLNQDTAVFLGAEKLCKQ
                     TNYPLIYFSMHQIRRGYYDITIIPISDDPKNTAEHELTHKHLRLLENDIHAQPGNWLW
                     SHRRWKY"
     gene            11233..11517
                     /locus_tag="PKY09_10155"
     CDS             11233..11517
                     /locus_tag="PKY09_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNB13257.1"
                     /translation="MNVLKQFRLVAIAEGISFLVLLFIAMPLKYGADMPLAVKYTGWV
                     HGLLFVFYIYALLQVWQQYKWNLRMMSLAMLASVLPFGTFYFEKKYLNNI"
     gene            11550..12578
                     /locus_tag="PKY09_10160"
     CDS             11550..12578
                     /locus_tag="PKY09_10160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB13258.1"
                     /translation="MIKAAVVILNYNGAALLQQFLPSVIANTPLWCSIIVADNASTDH
                     SLSVMKQHFPDVSCIEMQQNTGFAGGYNNALHQIDAEYFILLNSDIEVTPQWIEPVIE
                     FMDKHADVAACQPKILAYHQKEFFEHAGACGGYIDYLGYPFCRGRIFNSLEKDENQYS
                     DNKEIFWATGAAMFIRGHVFKSTGGFDTDFFAHMEEIDLCWRMKHSGHKLYCIPASKV
                     YHVGGGTLHKSNPKKTYLNFRNNLMMLLKNLPEGNRFSVFTKRLYLDKLAFIKFFFSG
                     NFKDAWAVYRAYFYVMLNYNTLKKKRGEIKNVNTNVSCIFEKSIVSEHFFRGKKKFTE
                     LNQDYFSK"
     gene            12596..13207
                     /locus_tag="PKY09_10165"
     CDS             12596..13207
                     /locus_tag="PKY09_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNB13259.1"
                     /translation="MKIICVGRNYAAHAAELKNPISAEPVIFMKPETAQLLPRMPFFI
                     PDFSTDIHHEVELVVKINRTGKHIQEHFANTYYDEIGIGIDFTARDKQSQLKEKSLPW
                     ELAKAFDGSAPTGKFLNKSIFTSVQNINFHLEKNGSIVQQGNSSNMLFNIDKIIAFVS
                     SYITLKKGDLIFTGTPEGVGKVQANDVLEAFIEDQHLLTVKIK"
     gene            13307..>14353
                     /gene="mgtE"
                     /locus_tag="PKY09_10170"
     CDS             13307..>14353
                     /gene="mgtE"
                     /locus_tag="PKY09_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNB13260.1"
                     /translation="MLQQQQKEEIKSLLREADLAGCKAFLENWLPSELVELIDESPNE
                     HRVLIFKALNREQAFESYESLDLNLQISLLDILPPRQVQLILNDMSPDARTALLEDID
                     NDLLNRLLKLLTPKERAIALSLLGYPENSIGRLMTPDYIAIRKEWSVQYALDYIRENG
                     ENSETLNIIYIVDDKGHLIDDIKVAEILLANLHVKIEELIDGKYTALSVTDDEKVAIS
                     EFKKHNRVALPVVDTSQMLLGIITVDDVLQLAEQEYSEDIQKMGAVEALEEPYMEVPM
                     YRLLQKRAPWLIVLFIGEMFTASAMSFFEHEIQRAVVLALFIPLIVSSGGNAGSQAAT
                     LIIRALALGEVTMKD"
BASE COUNT         4619 a         2673 c         2715 g         4346 t
ORIGIN      
        1 tacctgttgt tgcaacccaa gaactaccac tctttaaata agtaagaaca tttgttgttc
       61 ctaaactata gccctgtgaa gttatgcccg tcaaattaat ttctactaca gtgcttccac
      121 tactttgagc aataatagta ttataagcac cattcaccca cgaggagtcg gctttagtga
      181 gtgtagtaat tccgttctca ccataataaa aaccggcact ttgtgcaggt ggatttggtg
      241 aggcgttgtc gtcatccttc tttttacatg aagtaaataa tactacggat acaatagccg
      301 cagaaataac aaaattaaat agttgcattt ttttatgtgt ttaaattaca ttacttaaaa
      361 cgcaaattaa ccgttgacgt tacataaaac caatgggctg cacagcccat ttttaaaaaa
      421 gttaggctga tgaattgttg atttcgtact cgtaaaaaaa acttcaatgc ttcaatgccg
      481 ctgtcttttt cgtgctgcct cttctatctt aaaaaccatt gctaaaaaaa gacttcgtga
      541 atagcgaaag aacagtggaa ataatgcaat caataaaaca ctgttaatta ttaaataagg
      601 tgcaacaaca aatccccaca acagatacac ccaaataaaa ttaaagacag acatagcaac
      661 agttaatgca taactcacat acatggctcc gtagtaaaaa cccggctccg gtgtaaaagg
      721 taatgcacag ttaggacaaa caacaggcat gtcgcctaac tttttaaaat gataaggatt
      781 ggggtctgta aacaaatttg attgtgcgca ctttgggcat tttaatttta acgcagcccg
      841 cattggtgat gggttcatta tttagtgttg gttaattgtt gaacaaaata attccacaaa
      901 ggttcttcag gtggctttgc ttctatgcaa accggctcgg attttacagg gtgaacaaag
      961 gtcagtttac gagcatgtaa atgtatggag ccatcggcat tgcttctgtc ggcaccgtat
     1021 tttaaatcgc ctttgatggg gcagcctatg gcagaaagtt gtactcttat ctgatggtgt
     1081 ctgcctgtga gtggatttac ttctaacaaa tgataatgat cggaagatgc cagcagtttg
     1141 tacgacaatt cgcaacgtgt ggtaccattc ttttcctgta atgaagcgaa gcttttattc
     1201 tttgcttcgt ttttcaccag gtaatgaacc aaggtgtcct cctcttttgc aggtttgttt
     1261 tttaccactg cccaataggt tttttgtact tttctctcac gaaattgctc attcatacgc
     1321 tcaagtgctt ttcctgtttt agcaaataac acaacaccgc taacagggcg gtcgagtctg
     1381 tgtatggttc cacaaaatac attgcctggt ttttggtact tctcttttaa atattgcttt
     1441 acccaatcgc ctaatggtac atcaccggtt ttgtcacctt gtgttatctg cccgggaagt
     1501 ttgttgatgg caataagatg gttgtcttca aacaatacct gacttccaat atccggttgt
     1561 ttaaatcgga tttgttccat caatactgct ccttgctatt gggaaaatcg cgactcttaa
     1621 cgtctgttat atattttgcc gtagctgctt ttatgtcgtc atagaggttg agataacgtc
     1681 ttaaaaagcg cggatgaaac tcattgttga tgcccagcat atcatgcaac accaacactt
     1741 gtccgtcaac accgttgcct gcaccaatac ctatgacagg aatttttact tcttgtgaaa
     1801 ctattgctgc cagtgcagca ggaatttttt caagcacaat agcaaaacat ccggctgctt
     1861 ctaaaatatg tgcgtcttca atcaatcgtt ttgcttcttc ttcttcgcgt gcacgaacta
     1921 cataggttcc gaacttataa atagattgcg gtgttaaacc caaatgcccc atcacaggaa
     1981 caccggcagt cagaattctt tctatagact cctttacttc tcgtccgcct tcaagtttta
     2041 ctgcatgtgc acccgattct ttcatgatgc gaatagcaga ctgaagtgct tccttgctgt
     2101 tgccctgata ggatccaaaa ggtaaatcaa ctaccaccaa ggcacgttta actgcacgca
     2161 caacacttga tgcatgatat atcatctgat cgagtgtgat aggcaatgtg gtttcatgcc
     2221 ctgccatgac attagatgca gaatcaccaa ccaaaatgac atcaatacct gcatcatcaa
     2281 taatacctgc aaggctgaaa tcataagctg tgagcataga tattttctcg ccactgcgtt
     2341 tcatctcctg caaaacgtgg gtggttattt tttttacttc gctatgtacc gacatactta
     2401 taaaattaat tttcctttgg aagtgtaaac tctgtattta ccttctgagg ttcttttttc
     2461 tctgcaggcg gagctgcttt tgtacctttc tgtttcatca actcagtttt taatatctgt
     2521 aacgaatcag agagttgttt gtttttttct ttctctgctt ttacagattt actaagtgta
     2581 ataatcaaat cagcgttttc gtttccggca accatctctt tatacttcag caaagtctga
     2641 tttactttta aaagcgaatc gaatgatgct tcttttattg tcatttgctt ctgcaaatcg
     2701 gttatgattt tatcttttaa ctttctttca ttttcatctt cctggtcggc cgacttcact
     2761 gactctaaat tgcgtgtcaa ctcttcattc tctgcctgca acttattgat ttgtgttttt
     2821 agtttgatga tagactcagc aagttgaatt acatcaggac ttccctcttg ctgattgttg
     2881 ttgttactac tacctgctgt tgactcactt ttaccggatg cagaaaacaa ataaagaaaa
     2941 tctatcttgg cacgcgaacc cggtccaact atataaccaa agcgtccact cttgtaagca
     3001 atttcagtaa acgacatcag caggttgttg ttcagataaa tgtcgtagtt gcgttgtgag
     3061 aacctaagtt ccagaagatt gtaaacattc agattattga taaaattact ctttacccag
     3121 ccaccacttt tggcatcacc ggtgaggtat ctataactgc ctgccactat ctgacgcagc
     3181 ctgaattgtt tcaaggtatt tatctcaaaa ataaatccgc cattgccatc attctgagcc
     3241 ataaacataa cacccacaga gccattctga tcgtttgctt tttcaatttt cagtgaggta
     3301 atcatcttaa atgccggctg attgtagtcg tagttggcaa taatggcata aggtttttca
     3361 ggtgctttgc ggttaagaat atattcgcct tcctgtacta aaaaaagatt ctctgagttt
     3421 gacataaacg tccatatacc actgctggta tcgaaatttt cacttaaatc gaggcggtcg
     3481 aacttttcat ttactgtttg cgcctgcacc ggtgttaatt ttgacaaagc tacagcagta
     3541 aacatcagaa gaaaaagcaa tttatacatc aggcaatggt gatttttatg ataacgatat
     3601 gttaaaattt gcgtggtgaa aattacatat tttttttatc agccgtcatc ttctacattt
     3661 gaccccatga ataagttatt aatatctttt tgtttactct gtctgtctct gtcatcgtgc
     3721 aaaactgatg tggatttatt agctccctat aaggaagtga tggttattta cggtgttctt
     3781 gaccctactg acagtgttca atatatcaga atcaacaaag cttttcttgg cgaaggcaat
     3841 gcctatacgt ttgcacaggt agcagattca tttcaatatg ccgatgtgtt ggatgttaaa
     3901 ttagaacgct ataaaaacaa caactttgca ggcgccatca acttagagcg ttttgaaggt
     3961 cctccactaa aggacggcat ttttgcttct gccccaaatt atctgtatcg cacaacaggt
     4021 ctcgactcta tttatcagga cagtgaatac aaacttgttg tcaccaatac acaaaccgga
     4081 aatgttgtta cttcaaagac tgctgtagtg agtaaggccg gttcaataat taacccgaat
     4141 ccttcttccc tttatggata tgtaaatttt gctgatcctg ccaacccatt tgtaaccaaa
     4201 ttttcctcca gtgataaggc aaaatactat gacttaaaca taaaatttaa ttataaagag
     4261 attgaaattg caaacccttc taattttgag atgaaatcaa tttttttcag agttgatggt
     4321 gttgaaaaac aaagtaacaa cagccttgaa attcccgtat cttatactac attttattct
     4381 gctttgagga gattaattcc ggttaatcca tttgtaaaac gcgaagcaga accacttgat
     4441 tttgtattgg ttggtggtgc agaagagttg tacacatatt atcaggttaa ccgcccgcca
     4501 acaggcatca atcaatctat tccgcaatac accaatattg aaggaggcat tggcattttc
     4561 agtagtaaat atactgatat tgtttctcat aaatctttgt ctcaaacctc aatggactcg
     4621 cttatcaatg gcgccataac caaggatttg ggctttcagt aaccgtgtct ttttgtcata
     4681 tttttaatta gttaccttag tgatttgact gtaatgacag taaaatgtca taaatagtcc
     4741 attggcacaa ggcttgacaa taggataaaa aacctctaaa agtatttaag aaaatgagaa
     4801 aagttattgg aattgacctt ggaacaacca actcatgcgt tgccgtaatg gaaggtaacg
     4861 aacctgtggt tattccgaac agcgaaggtc gcagaacaac cccatccatt gttggattta
     4921 ttaaagatgg cgaacgtaaa gttggcgatc ctgctaaacg tcaggctatc accaatccaa
     4981 aaaatacggt ttattctata aagcgtttca tgggcgaaac ctatgatcat gcaagcaaag
     5041 aaaaagaccg cgtaccttac gaagtggtga aaggggataa caacacgcca cgaattaaaa
     5101 ttaacgatcg ccaatataca ccacaagaaa tttctgccat tatacttcag aaaatgaaaa
     5161 aaacggcaga agattatttg ggcaatgaag ttacagaagc agttattact gtgcctgcat
     5221 acttcaacga ctcacagcgt caggcaacaa aagaggccgg tgaaattgca ggtctgaaag
     5281 tgttgcgtat tatcaacgaa cctactgcag cagcattagc ttatggtctt gacaagaaaa
     5341 ataaagacca gaaaattgta gtgttcgact gcggtggtgg tacacatgac gtttctgtgc
     5401 ttgaactagg tgatggcgtg tttgaagtca aatctactga cggtgataca caccttggcg
     5461 gtgacgactt tgaccagaga ataattgact ggttagccga tgagtttaag agcgatgaag
     5521 gtattgacct tcgtaaagac cctatggctt tgcagcgctt gaaagaagct gctgagaaag
     5581 ctaaaattga attgtcaagt tcaacacaag ccgaaattaa tctgccatac attatgcctg
     5641 ttgatggtgt tccaaaacac ttggtaaaaa ccattacacg tgcaaaattt gagcaattgg
     5701 ttgatgattt aatcaagcgt accattgaac catgtaaaac agcattagaa aatgcaggat
     5761 taaaaccttc tgatattgac gaagtgattt tagttggtgg ttcaacacgt attcccggtg
     5821 tacagtcagc agtagagaaa ttcttcggta agtcaccttc aaaaggtgtt aacccggatg
     5881 aagtagtagc tattggagca gccatacaag gtgctgtttt aaccggagaa gtgaaagatg
     5941 tgttattact tgacgttaca cctctgtcac tcggtattga aacatttggt ggtgtgttta
     6001 ccaaacttat tgaagctaac acaaccatac cgactaagaa aagtgaagtg tttagtactg
     6061 ccagcgacaa tcagccatca gtagaaattc acgttctgca gggtgagcgc agcatggcaa
     6121 aagacaaccg aaccatcgga cgttttcact tagatggtat tccacctgca ccacgtggtg
     6181 ttccacagat tgaagtaata tttgatattg atgcaaacgg cattctgcat gtaacagcaa
     6241 aagacaaagg caccggcaaa gaacaaaaaa tacggattga agcaagcagc ggattgagtg
     6301 atgatgaaat caaacgtatg aaaactgaag ccgaagctaa tgcagaagct gataaaaaag
     6361 caaaagaaga agccgataaa gttaacatgg ccgacaccat gatattccag acagaaaaac
     6421 agctgactga atatggcgat aaactttcac agagcaataa agaaaatata cagcgtgcat
     6481 tgagcaaact caaagatgca cataaggaga aaaaccttgc tgatattgac actgccctga
     6541 atgagttgaa cacagcatgg caaggtgctt cacaagaaat gtataatgct accaacaaca
     6601 cagctaacac aacagcaggt ggtgccgatg caaatataaa tgctgaaaca caaaacaaca
     6661 ctgcaggcga agtaactgat gttgactttg aagaagtaaa agacgataag aagtaattgt
     6721 agattttggt ttttagataa tccgtcctga tgattcggga cggatttttc attttatgcc
     6781 atacttcaat atcaacttac ataaatcttc aacgtgattt tatttacaag gaaacttggt
     6841 acgcatgtgg ctgtgtattg cagttgtttt gctgctatat ccccacatgt acatttctga
     6901 agttaaaacc ggtgcaaaag gaatgtgtgg atgatgtttt attcttttta gtataaactt
     6961 atttcaggtc aagaagaaac ctttaccctt agcattgcaa ggtggacaat tacagcctat
     7021 gggatttaca ggcaatgacc aagtctatct gatagctggc atgtcatgtt aaatattatt
     7081 atttgtgaac aagttatatg tgttatattt gcatcacaat ttaacacgtt atacatgaag
     7141 ttcgagaagc cacctgttga tgaaacattt gatattaaaa tagttaaact gattatagat
     7201 gagcagtata aagattttct ccaaactgta gagtccaggt atttgtactg ggatgaagtt
     7261 aaaaacagga aggatttgcc tttcgaccct gccaagtcat ggaggttaat aaaaacgagt
     7321 agagcactta attctaagag tgtaaatatt ggagcatacg tctttaagta ttatttgact
     7381 tatcaaattc aaaaagattt acatgaattt gatttaaaga tgataggtgg attatatcag
     7441 aatccgataa cagattttga aaaagcagaa tatataaaaa actctttgat ggaagaagca
     7501 attgcatcat cgcaaattga aggtgctgca acaacaacca aaattgcctg ggatatgctg
     7561 aagtcaggaa ggaacccaag aaatgagtcc gagcaaatga tatttaataa tttgcgagct
     7621 ataagatata tagaagagaa tcaaaataaa aatattgaca aggcttttat tatcgagtta
     7681 cataaaatta tgaccgtaaa aacaactgct gaagattgtg ggggagatta ccgtaaagga
     7741 gagatatttg ttcaagatca tgttgatggt gaaattgctc atattcctcc agattggaac
     7801 gaactaggaa gcttaatgga tagtctctgc agttttgcaa attcagataa agaatttatg
     7861 catcccatta taaaagcttc aatgattcat tttttcatcg gatacattca cccgtttaaa
     7921 gatggaaatg gaagaacggc aagagcattg ttttattggt acttgttaaa aaaaggatac
     7981 accctaataa aaaatatttc gatttcaaga gcaattctat cgtcaaggat tcaatatgat
     8041 aaggcgtttt taaaaacaga gtatgatcaa aatgatttga attattttat tacatattca
     8101 atcaaaagtc tacgcgtagc atttgaaaat cttgtacggt atagagataa gaaaaaagct
     8161 gacaaggaac aggcatctgt tattgcttac agactaattg aaaaagggat gtctaagaga
     8221 caagcagatt tattagggta tttatattcc aaggagagtg cggcagtttc tcttcagcaa
     8281 tatgcgataa aacacgacat agtccgccaa acagctagaa aggatttgtc gcacttagta
     8341 aaattaaatt tactcattga ggaaaaaaaa ggaaagtcta ttgtttttcg tgttaagtca
     8401 aagtccgatg tcgaaaaatt tcttgacaaa taattacacc caacagcagt ccatcaataa
     8461 attaccctat tgttttcaaa gacaaaaaaa taacttttca aacgcatcaa gaacgattta
     8521 aactactttt aaaagtcata tgatatacat tggaatcaat aatatagatg cccaatacaa
     8581 cacacaaatt gtttcaaatg aatttgggga gcatactggg gagcattgca ctttcccctt
     8641 tgcagaatac aatatgtaaa caaaaaacat atcttacctc ttaaactaaa aaagccccct
     8701 atcggaagcc tctttttgcg gagagagagg gattcgaacc cccgttggtt ttacccaagg
     8761 cggttttcaa gaccgctgca atcaaccact ctgccatctc tccgggcgcg aaagtaatac
     8821 aatttttaat tcgcaaatta attttgacat ttttacaaca cttatactta aaatcttgtc
     8881 tatctttata ctctaaataa ttcatacaat gaaacgctta ttattattct tagcacttgg
     8941 gtctatagca gcgctgactc aggctaaaac aattaaggct ttgtttgatt ataaatcctt
     9001 ctatgctccc ggtcagggtt cgtacctgga atggtatctg tctgtaaaag gcaattccgt
     9061 aaactacaaa atgagcgaaa gcggcaacta cgaagccgca gtggcagtag cttataaaat
     9121 tacacaaaat gataaaattg ttgcgcagga cagctataac ctcatcagcc cgctgacaaa
     9181 agacacgctc cttgtatccg attttattga caataaaaga ttgcagctgc ccaatggaaa
     9241 gtataatatc gagctatcag taaaagacaa tgtagcagat tccaagacct ttaccatcaa
     9301 tcagggattt gaattggctt ttgaaccggg aaaaacctct gtttctgata ttgaactggt
     9361 gcagcattat tcaaaaaagg aaaccggcaa taatacttta gttaaaaatg gatttgaaat
     9421 aattcctcgc gttgacaatt tttatcagac aaacgaagaa cgtttaatct attatgcaga
     9481 agcctatttt acaaactcac taactgatga tgccgttgcc gtttacagtt atctgcaaaa
     9541 tgcagaatca ggcagcacta taatggatta cggaactgtg cagcgtaaaa aaacaagtga
     9601 agtaattcct ttgttgagtg aaattaatat tgctaatctg ggttccggca actacaatct
     9661 gatagttgaa gtaaaagaca aaaacaatca atcgttggca ttgtctaaaa tattttttca
     9721 gcgtcaatct gtagtgaaag ataattttgt tttgaataat gatgtttcat caacctttgt
     9781 tgccacaatg aatgataaaa atgtactggc agaaaatata gcctgtctgc agcctatcag
     9841 caatgcacaa caatcggagt ggataggcaa tcagttgaaa gctgccaata ttgatatgat
     9901 gaaaaatttc ttttatgact tttggcaacg cagagagcct gtcgatccgc aaaaagcatg
     9961 gaacgattat aaagcccatg tagatgccgt tcagcaaaaa ttcggttctc gtgcattaaa
    10021 aggttacaat actgatatgg gaagagttta tcttgcttat ggcgcaccaa gcaccataaa
    10081 taaaggcgaa tacgagccta atacctatcc gtatgaaatc tggcgctacg ataaaataaa
    10141 caatcacact aacaagactt ttgtgtttta cagcaaagat ttgttagcac gtaattttac
    10201 actcttgcat agcgatatgc ccggagagcc ttatgaagca gcatggaata tgattttgca
    10261 tgatcgtagt attaaagcta aaaatattga tgataccggt aaggatttga aaggtgttta
    10321 ttcgggcgac agaacaaatg ataattttaa tgccaagtaa tttttaggtg gcctttctct
    10381 ttctgaagtt tcttattcgc tgtattgctt ttcttgactt aagaatactt tatctcctgg
    10441 cagatgttat tgcattttgc tttaaatata ttttccctta tcgcaaaaaa gtaattcttc
    10501 aaaatcttta caactctttt cccgaaaaaa acgaacagga gattgaagaa attgcaaagc
    10561 agttttacaa ttactttgcc gacttaattg tagagaccat aaaaatgtca agcatatcaa
    10621 aagaagaatt gctcagacgt tgccgtttta ccaatcccga aattcttcat aactattatg
    10681 aaaaaaaacg cagtatcatt gcagtatgtg gtcatttcaa taactgggag ttaggagctt
    10741 tggcactttc tgtcatagac aaacatcaga tgcttggagt ttacaaacca ctgtccaaca
    10801 attcgtggaa tgaatatttt aaaacaatac gttcaagatt cggtatgata ccggttccga
    10861 tgaaatcaat cttacgcgaa ttgattaaac gcaaaaatga acctaccata actgcccttg
    10921 ctgccgatca gacaccacac cgttcagaga taaattatcg aaccacgttt ttaaatcagg
    10981 atacagcggt atttctaggt gcggaaaaac tttgtaagca aaccaattac ccgctgattt
    11041 atttttctat gcatcaaatt cgcagaggtt attatgatat aaccataatt cctataagtg
    11101 atgaccctaa aaataccgca gagcacgaac ttactcataa acatttaaga ctattagaaa
    11161 acgacataca tgcacagccg ggcaactggc tttggagtca ccgcagatgg aaatattaaa
    11221 tatcaatcaa taatgaacgt attaaaacaa ttcagattag ttgcaattgc agaaggaatt
    11281 tcctttttag ttctactttt tattgccatg ccgctgaagt atggtgctga tatgccatta
    11341 gctgtaaaat atacaggctg ggtgcatgga cttttatttg tgttttatat ttatgcgctt
    11401 cttcaggtgt ggcaacaata taaatggaat ttgagaatga tgtcgttagc tatgctggca
    11461 tcggttttgc ctttcggaac attctatttt gaaaagaaat acctgaacaa catctgatat
    11521 ttactcctta gatttcattt ctttgcagcg tgattaaagc tgctgttgtc atattaaatt
    11581 ataatggtgc tgcattactg caacaatttc tgccctctgt aattgccaac accccgttat
    11641 ggtgttctat tatagttgca gacaatgcct ctaccgacca ttccttatct gtaatgaaac
    11701 agcattttcc ggatgtaagc tgcattgaaa tgcaacaaaa caccggcttt gccggaggct
    11761 ataacaatgc tttacatcaa atagatgcag aatactttat tttactcaat tctgatattg
    11821 aagtaactcc gcaatggata gaacctgtaa ttgagtttat ggataaacat gcagatgttg
    11881 cagcatgtca gccaaaaata ttagcctatc atcagaaaga attttttgaa catgccggtg
    11941 cctgtggcgg atacattgat tatttgggtt atccattttg cagaggaaga atttttaatt
    12001 ctttagaaaa agatgaaaac caatactccg ataataagga gatattttgg gcaacaggtg
    12061 cagctatgtt tatcagaggc catgtcttta aatcaacagg tggttttgat actgactttt
    12121 ttgcacacat ggaagaaatt gatctttgct ggcgcatgaa acacagtggt cataaattgt
    12181 attgcattcc ggcatcaaaa gtgtatcatg ttggtggtgg aacattacat aaaagcaacc
    12241 caaaaaaaac ttatctcaat ttcagaaata atctgatgat gcttttgaaa aacttacctg
    12301 aaggcaacag gttttctgtt ttcacgaaac gattatatct cgataaattg gcatttatta
    12361 aatttttctt ttctggaaat tttaaagatg catgggctgt ttacagggcc tatttttacg
    12421 tgatgcttaa ttacaataca ttaaagaaga aacgtggaga aattaaaaat gtcaacacca
    12481 atgtttcatg tatatttgaa aaaagtattg tttcagaaca ttttttcaga gggaaaaaga
    12541 agtttacaga gttaaatcag gattattttt caaaataatt tttaattgct aatcaatgaa
    12601 aataatttgt gtcggtcgaa actatgctgc tcatgcagca gaattaaaaa accctataag
    12661 tgcagagcct gtaattttta tgaaacccga aacggcacag ctgctgccgc gaatgccgtt
    12721 ctttattccc gacttcagca ccgacattca tcatgaagtg gaattggtgg taaaaatcaa
    12781 ccgtacaggc aaacatattc aggagcactt tgcaaacacc tattatgatg aaataggaat
    12841 aggaatagat tttacggctc gcgacaaaca atcgcaatta aaagaaaaaa gcctgccctg
    12901 ggagttagcc aaagcatttg atggcagcgc accaacaggt aaatttctga acaaatcaat
    12961 ttttacgtcc gtacaaaaca ttaattttca tctcgaaaaa aacggcagta tagtgcagca
    13021 gggcaatagc agcaatatgc tttttaatat tgataaaata attgcatttg tttcatccta
    13081 tatcacactt aaaaaaggtg atttaatctt tacaggtaca cccgaaggtg ttggcaaggt
    13141 acaagccaac gatgttctgg aagcatttat tgaagatcag catcttctga ctgttaaaat
    13201 aaaatagtta aaaaaataat tattcattat cagaacatca agtcaatacg ctaattttac
    13261 ccttataaat atttaaattt acgcccgcaa aaatcacttt tcaaacatgc ttcagcaaca
    13321 gcaaaaggaa gaaataaaaa gcctgcttcg ggaggctgac cttgcaggtt gcaaagcttt
    13381 tcttgagaat tggttaccct cagagttggt cgaacttatt gatgaatcgc ctaacgaaca
    13441 tcgcgtttta atttttaagg ctttaaatcg tgaacaggct tttgaatctt acgaatcgtt
    13501 agacctgaat ctgcagattt ccttactcga tattttacca ccacgtcagg tgcagcttat
    13561 tctcaatgac atgagtcctg atgcacgtac tgcattgctc gaagatattg ataatgacct
    13621 gctgaaccgt ttgctgaaac tgctcacacc aaaagagcgt gctattgctc tttcactact
    13681 tggttatcct gaaaattcta tcggtcgtct gatgacgcca gattacattg ccatcagaaa
    13741 agaatggagt gtgcaatatg cacttgacta tattcgtgaa aatggtgaaa attctgagac
    13801 actaaacatt atttatattg ttgacgacaa agggcatctg attgacgata ttaaagtggc
    13861 cgaaatttta ttggccaacc tgcatgttaa aattgaagag ctgattgacg gaaaatatac
    13921 tgcactttct gttactgatg atgagaaagt tgccatcagt gaatttaaaa aacataaccg
    13981 tgtggccttg cctgttgttg acacttcgca aatgctgtta gggattatca ctgttgatga
    14041 tgtgttgcaa cttgcagagc aggaatattc tgaagatatt cagaaaatgg gtgctgtgga
    14101 agccttggaa gagccttaca tggaagtgcc tatgtaccga ctgttacaaa aaagagcacc
    14161 ctggctgata gtacttttta ttggtgaaat gtttacagca tcggcaatga gtttttttga
    14221 gcatgaaata caacgtgccg tagtacttgc cttgtttatt ccactcattg tcagtagtgg
    14281 cggtaatgca ggttctcagg cggccacgct tattattcgc gcattggcac ttggcgaagt
    14341 aactatgaaa gac
//
LOCUS       DAOQNP010000077        14090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1539_length_14090_cov_8.199359, whole genome shotgun sequence.
ACCESSION   DAOQNP010000077 DAOQNP010000000
VERSION     DAOQNP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 14090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14090
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1539_length_14090_cov_8.199359"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKY09_10175"
     CDS             complement(<1..749)
                     /locus_tag="PKY09_10175"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNB13261.1"
                     /translation="MTEKSNNGFYIKVVVPLPLPNLFTYSVPDTLISQIEIGKRVLIQ
                     FGSQKIYAAIVIETAVDKSNDYEVKPIISIIDEMPVVTEKQIAMWHWVHSYYLCFWGD
                     VMQAALPAELRLSSETKIVLNTEKESGSEYLTDDEFLILEALHSVKELSLKEISRILD
                     RKHVFPVVKTLLQKKLVLSVENIGEDFKMPMANFIRRTEQTNDQDFLKQQFDLLEKKA
                     PRQLDVLMQLLLLEKNYSEGKVPQKVLLHKSG"
     gene            complement(746..1873)
                     /gene="corA"
                     /locus_tag="PKY09_10180"
     CDS             complement(746..1873)
                     /gene="corA"
                     /locus_tag="PKY09_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNB13262.1"
                     /translation="MAKIRRLSVKLGRKKTTTPGAHPGSFSIPSDALVPKALCFIYDN
                     DSFQEIDVTQFDQLKNAYQELGEKTLWLHVSGFGDKSFYEDLASLFQLHRLELEDVFN
                     VYQRPKVDEYEGHLFFVSRLLSMTDETLNNDQLSLILGKNFVISIQEKHQDFFDPIRQ
                     RLRNGKGKMRTLQAGYLAYALMDAVIDYYYPALEKIGLKLDDLEDELLSNPSQDSMNR
                     ILQIKRELIVFRRAIWPERDKINDILRSSFDEVPEETKLFFRDSYDHCIQILDIVESY
                     KEVTASLMDVYLSSVSHKLNQVMKVLTIISTIFIPLTFIVGLYGMNFQLTNPETGESL
                     PMNMPELYSPYGYIGVIAVMLLIVVVQLYIFHRKGWIRFRE"
     gene            1940..2671
                     /locus_tag="PKY09_10185"
     CDS             1940..2671
                     /locus_tag="PKY09_10185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB13263.1"
                     /translation="MKKWFQSWFDSPYYHILYKDRDNQEAEYFMDNLLEQIHLPINSS
                     ILDIACGKGRHSMFLADKGYHVTGIDLSEQSIEYCKQFERQSLEFYVHDMREIFRTNC
                     YDVALNLFTSFGYFNSDHDNHLALRTASMSLKPGGYFILDFFNPNLIKSKTPQQHIKK
                     VEGIDFSIYKYSDGNTIEKTIHFNDAGKDFKFTESVQIISKNTFLNHFNRVGLITKHI
                     FGDYALNPFEEKSSERMIFITQKPQ"
     gene            2668..3363
                     /locus_tag="PKY09_10190"
     CDS             2668..3363
                     /locus_tag="PKY09_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNB13264.1"
                     /translation="MTASFIVLFLLITGGGLLYFLLNKSNDALLKLSLAFSGAYLFGI
                     TITHLVPEVFSQGDKSIGIYIMAGFIFQIILEYFSKGIEHGHIHLHENKSHAVFPVSM
                     MISLCIHAFFEGVPMADEQQRQSLMMGIAMHHIPVAFALMSMLMQSGVSKTISVFSLV
                     VFAAMSPVGAFFGIYLGDTLMTEWFSKIMAIVIGIFLHISTTILFESDSNHRFNFIKM
                     AVILAGVGLSLMV"
     gene            complement(3423..3872)
                     /locus_tag="PKY09_10195"
     CDS             complement(3423..3872)
                     /locus_tag="PKY09_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNB13265.1"
                     /translation="MTLIKFKNEPASRMMDRSPLFSDLFNDFFEGVVSNNITKSFSPS
                     VNVKESDHEFKLELAAPGLSKDDFKINIENDMLNISAEKKEEQATQNEKYTRKEFSYA
                     SFKRTFTLPETADSEKISAAYDNGVMTLVIPKKDEARPKPLREIKIS"
     gene            4159..5214
                     /locus_tag="PKY09_10200"
     CDS             4159..5214
                     /locus_tag="PKY09_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNB13266.1"
                     /translation="MKASRFLLIGLIIAGLIAVKLIFFPGTKTSKFGGKANAAAVPVT
                     GFIVRSQVLESNLKANGTINAYEQTDLRAEISGRIVAILFKDDQPVKKGQLLIQIYND
                     DLKANLSKSTAVLKQTQDRLKRQEKLLEIGGVSTEEVEETQTEAATQKAEIDYLNFQI
                     TKTEIYAPFDGIVGIRDISIGNYVNSSAVIATVYQVNPLKIDFTVPEKYLPYISKGMK
                     IRFTTDVNKKEYEGEIAAINPMIDPSTRMVRLRAYTSNSDHTLTPGSFAHIQVDFKIT
                     ENAILIPTQAVIPILKGKKVFVSKGGKAEEKIIETGERTADMIEVTTGLTVGDTVLTT
                     GILQVRKGSDIKFSEVK"
     gene            5219..8284
                     /locus_tag="PKY09_10205"
     CDS             5219..8284
                     /locus_tag="PKY09_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNB13267.1"
                     /translation="MDLSEISVKRPVLAIVMSLVILLFGIISYTFLGVREYPSIDPPI
                     INVRTSYPGANADIIESQITEPLEKVINGIQGIRSISSTSSQGTSNITVEFNLNSDLE
                     AAANDVRDKVSQAVRTLPRDIDGLPVVTKSDANADAIISLTVQSNTRNTLDLSDYAEN
                     VIGQQLITIPGVSTLQIWGQRKYAMRLRIYPDKLAAYKLTVQDIQNALNRENVELPAG
                     KIAGDNTELVVRTTGQLHSEDDFNNMIIVNNGDQLIRFRDIGLAELGPENEETILRES
                     GIPMVGIALVPQPGSNYIEIADEFYKRLEKIKVDLPSDIKIGIALDNTTFVKKSITEV
                     EETLFIAITLVVIIIYLFFRDWLVAIRPLIDIPVSLVGAMFIMYIAGFSINVLTLLAI
                     VLATGLVVDDGIVVTENIYKKIEAGLSPNKAAIEGTKEIFFAVVSTSVTLAAVFLPVV
                     FMEGFVGRLFREFGVVIAGAVLISAFVSLTLTPMLNARLVRKDKKHSKFYEKTEPFFQ
                     RMNQAYENSLKWFMEARWRAWTILGSAIVIIFILGRSLPSELSPLEDRNWLRLLITAP
                     EGASYEYTDNFIVKISDVIEDSIPEKNGLLTVTAPGFTGAGAVNSGFARIVLTTPDKR
                     ERSQQEIADQLSGITRKMTDARVFVLQEQSLSAGGSRSALPVQFVLQNQEFDKLRDVV
                     PAFLDRANKSPVFQGVDVNLKFNKPELNVSIDRAKANALGVSVDDIAQTLQLAFSGQR
                     FGYFIMQGKQYQVIGQVDRENRDVPYDLSKLYVKTNSGGLVQLDNLIEVNEISSPPQL
                     YHFNRYQSATISAGLASGYTIGDGINEMKNIGDSLLDESFSTDLTGPSRDFAESSSNV
                     WFAFVLAIILIYLVLAAQFESFVDPFIIMITVPLAICGALISLWYFNQTLNIFSQIGI
                     IMLIGLVTKNGILIVEFINQLREKGVERSEAIIHGSVSRFRPILMTSLATSLGALPIA
                     LALGAGAKSRMSMGIVIVGGVLFSLIFTLYIIPAMYSVFSRKKEKVIYD"
     gene            8277..9575
                     /locus_tag="PKY09_10210"
     CDS             8277..9575
                     /locus_tag="PKY09_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNB13268.1"
                     /translation="MIKVRLTVLICFFFVQAQSQQVFTLQQALEKALNDNFSIRIARN
                     QSTILNNNATLGNAGMLPQVNLNGSYVKANNNVVQELSNGTSTEKQNAGQSTIAGSAV
                     LEWVLFDGLTMFSEYNKNSALSKQGKEQLKANIEDVIENVSKTYFTVVKEKLLLSSIN
                     ETLKFYADRLQIADSKYKIGSASKLDYLQSKVDYNAMLSQKLNKETDVNNAMIELKRL
                     LQFSADTMLQTEDTITFMAMSSMSDLLNGLQQNTAMQVSKLNVEVNRLQLNSMKGLRM
                     PVITGLAGYYYNESRNDAGLFSLNRTNGPQFGITARYNLFNGFNNSRQISNSKLNYIN
                     SQIEYQQTRTNQIADFNNTWNAFQKAQEILNLEIENTAAARENASVYLASYRLGSANL
                     LQLNQSQESLYDALVRLVNAQYNNKTAEIHLLRLAGKLIK"
     gene            complement(9588..10466)
                     /locus_tag="PKY09_10215"
     CDS             complement(9588..10466)
                     /locus_tag="PKY09_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNB13269.1"
                     /translation="MKINHLKLLVLIFSAIVLFISCEKDITVNLPKQKQQYVVEGYIE
                     NGLPAYVMLSKTADYFSPVDSTALQQYMVKNATIVISDGDITDTLLQVDPSIGYFYFS
                     PKIIGQTGKNYSLRIITPDNELLTAETKMLPAVPLDSVWFKIQEGKDSLGYVWAHLKD
                     PDTLGNCYRWFAKRLNKDKMFIAPSGSVFEDKFINGKAFDFAYNRGHLPNSTASEDEN
                     EEAGFYKTGDTIMVKFCTITEESYKFWRQAESQAGSSGSPFGSPAVFPTNIKGGIGIF
                     EAFNPTFYTVIAKKPD"
     gene            complement(10472..12901)
                     /locus_tag="PKY09_10220"
     CDS             complement(10472..12901)
                     /locus_tag="PKY09_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB13270.1"
                     /translation="MIRKYSLFLLLFLQIQILNAQDKPTRFTISGYVKDAATGEALIG
                     ANVYLRETMKGVQTNTYGYYSITVDAADSITLMSSFVGYSDYMQKMHITKDVRININL
                     RNKYIETQEVVITAKKEDQNVNSTQMGTVQLDVEQIKTLPAFMGEVDILKTIQLLPGV
                     QSAGEGNTGFYVRGGGPDQNLILLDEALVYNASHLFGFFSVFNSDAINNVSLIKGGMP
                     ANYGGRLASVLDISLKEGNNKEFKAEGGLGPIASRLTLQGPIQKEKSSFIVSGRRTFI
                     DLFQRPPFVKKGSNAANNTYYFYDLNAKINYQLSDKDRLFLSGYFGRDVFNFKSADTG
                     FKFRIPWGNATAVLRWNHLFSDKLFVNTSIIFSDYNFAFSAKQESFSFNIFSGIRDYN
                     AKIDFNWFPIITHNVKFGVNYTYHRFTPTSAEASQDSTEFDLGGVNRLYAHEAAAYIN
                     DDWTISDKFSVSAGLRYSLFDFTGPFTRYVKNEDNETVDTIYYKTNETVGFYHGLEPR
                     ISAKFSLDKNSSIKASFTQNYQYVHLASFTSVTLPTDVWVPSSDRVKPQDGRLYAAGY
                     FRNFHQNMFETSVEVYYKTMRNQIEYREGAQADDDVKNNTDNNFVFGKGWSYGAEFFI
                     KKAKGKLNGWVGYTLSFTDRKFPDIMNGKTFPAKYDRRHDISVVVSYNASKSWTFGLT
                     WVYATGNATTLPVSRYFMFGPLDLQGVVSSGLPNMSSGQIYDVYGDRNSYRMAAYHRL
                     DLSATYIRKKTKRFESSWNFSIYNVYSRMNPYFIYFNNEVDKETGEFKIQAKQVSLFP
                     IIPSVTYNFKF"
     gene            13101..>14090
                     /locus_tag="PKY09_10225"
     CDS             13101..>14090
                     /locus_tag="PKY09_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNB13271.1"
                     /translation="MKKLHVSFLVFLIFNLQGAFAQDMSKINFSLAKKISGPYPATRP
                     LALFVQGDVGVIQNYIQQNKGEVKFVAGDIVSLVMPLGAVNGLIALPSVKAVEDNGMQ
                     LVALDDSMRVRNNINPVQNGMAPLPQGYDGSGVVMGFIDSGIDFSHPDFKHANGSTRV
                     LYIWDHNLSVGAAPMPYNYGTEFTAADIDAGNASAHVDNSNGHGTHVTGMGAGNGLAL
                     NDHKGVAPAADIISVCVNWNLTDNDWLTTVADAVNYIYSKAYALNKPCVINISAGTYY
                     GSHDGKDLQAQAISNLISQRNGRSLVCAAGNAGNLPIHVQHLHTAANDTSFTWF"
BASE COUNT         4401 a         2426 c         2670 g         4593 t
ORIGIN      
        1 cctgatttat ggagcagcac tttttgtggt actttacctt cgctataatt cttctctaac
       61 agaagtaatt gcatcaacac gtctaattgt cttggcgctt ttttttcgag aaggtcaaat
      121 tgttgtttta aaaaatcctg atcattagtt tgttctgttc tcctgatgaa gtttgccata
      181 ggcattttaa aatcttcacc aatgttttca acggatagta ccagtttttt ctgaagtaat
      241 gttttaacaa ccggaaatac atgtttgcgg tcaagaatac gtgatatttc tttcaggctt
      301 aattctttta cagaatgtaa agcttccaat atcaaaaatt catcgtcagt cagatattca
      361 cttccacttt ctttctcagt gttaagtaca atttttgttt cgctgctcaa acgtaattct
      421 gccggtaatg ctgcttgcat tacatcaccc caaaagcaca aatagtaact gtgaacccaa
      481 tgccacattg caatttgctt ctctgtaaca acaggcattt catcaattat tgatattatt
      541 ggctttactt cataatcgtt agatttatct actgctgttt ctataactat agcagcataa
      601 attttctgac ttccaaattg tatcaatact cttttcccaa tttctatttg cgaaataaga
      661 gtatcaggta ctgaataggt aaataaattt ggtagtggta acggtactac tactttaata
      721 taaaatccat tgttgctttt ctctgtcatt ctctgaaacg tatccaaccc ttgcgatgaa
      781 aaatgtacaa ttgaactaca actatcagca gcattacagc aatgacacca atatagccat
      841 aaggcgaata taattcaggc atgttcatag gcaaactttc tcctgtttcg ggatttgtta
      901 gttgaaaatt catgccataa agaccaacaa taaatgttaa tgggataaaa attgttgaaa
      961 ttatggtgag caccttcatc acctgattga gtttgtgact aacactggat aaatatacat
     1021 ccattaaact tgctgtaacc tccttgtagc tctcaacaat atcaagtatc tgaatacaat
     1081 ggtcgtaaga atctctaaaa aacaattttg tttcttcagg tacttcgtca aatgatgaac
     1141 gtaaaatgtc gttaatttta tctctttcag gccaaatagc tcttctgaat actatcaact
     1201 cccttttaat ctgaagtatc ctgttcattg aatcctgtga tggattactt aacaattcat
     1261 cttctagatc gtctaattta aggcctattt tttcaagtgc cggatagtaa taatcaataa
     1321 cagcatccat caaagcatat gcaaggtaac ctgcttgaag tgtacgcatc tttcctttgc
     1381 catttctcaa gcgctgacga atgggatcaa aaaaatcctg atgcttttcc tgaatggaaa
     1441 tgacgaaatt ttttccgagt atcagactta actgatcgtt attaagtgtt tcatcagtca
     1501 tgctaagcag tctgcttaca aaaaataagt gaccctcata ttcatcaact tttggacgtt
     1561 ggtacacatt aaaaacatct tccaactcta atctatgcaa ttgaaaaagt gaagcaaggt
     1621 cttcataaaa agatttatct ccaaatcctg aaacatgcaa ccaaagtgtt ttttcaccta
     1681 attcctgata tgcatttttt aactggtcga attgagtaac atcaatctcc tgaaaagaat
     1741 cattgtcata tataaaacaa agtgccttag gtactaaggc atctgaagga atagaaaagc
     1801 ttccgggatg tgctccgggt gttgtggttt ttttacggcc gagttttaca cttagtcttc
     1861 tgatctttgc cattattaat taaaatgagg ttgtcaaaaa tagcgataaa tttgccggct
     1921 gaagaaagaa aagtgtttta tgaaaaaatg gtttcagtcg tggttcgatt caccttatta
     1981 tcatattctg tacaaggata gagataatca ggaagcagaa tattttatgg ataatctatt
     2041 ggagcaaatc catttgccaa taaattcaag tattttagac attgcctgtg gcaaaggtcg
     2101 tcacagtatg ttccttgccg acaagggcta tcatgttact ggcattgatt tgtcagaaca
     2161 aagtattgaa tattgtaaac agtttgaaag gcaaagtctt gaattctatg tccacgatat
     2221 gcgtgaaatt ttcagaacca attgctatga tgttgcacta aatttgttta ccagttttgg
     2281 ttatttcaat tcagaccatg acaatcattt agctttacga acagcatcta tgtctttaaa
     2341 acccggtggt tactttattc ttgatttttt taatcctaat cttatcaaaa gtaaaacacc
     2401 acaacaacat ataaagaaag tagagggaat agatttttca atttataagt actctgatgg
     2461 gaataccata gaaaaaacaa ttcactttaa tgatgcaggt aaggatttta agtttacaga
     2521 atcggttcag attatttcaa aaaatacttt tctaaaccac tttaatcgcg ttggcttgat
     2581 tacaaaacat atctttggcg actatgcttt aaacccgttt gaggaaaaaa gtagtgaacg
     2641 aatgattttt ataacacaaa aaccacaatg acggcatcat ttattgtttt atttctgctg
     2701 ataacaggtg gtgggctgct ttatttttta ttgaataaaa gtaacgatgc attgcttaaa
     2761 ctatctctgg cattttcagg tgcttatttg tttggtatta ccatcacaca tttagttcct
     2821 gaagttttta gtcaaggcga taaatccatt ggcatttata ttatggccgg ttttatattt
     2881 caaattattc ttgaatattt ttcaaaggga attgaacatg gacatattca tttgcatgag
     2941 aacaaatctc atgctgtgtt tccggtatca atgatgatta gtttatgtat tcatgcattt
     3001 tttgagggtg tacctatggc tgatgagcag caacgccaat cactcatgat gggcattgct
     3061 atgcaccata ttccggttgc atttgcttta atgagtatgc ttatgcagtc aggtgtttca
     3121 aagacaattt cagttttttc attagtagta tttgcggcaa tgtcaccggt gggtgctttc
     3181 tttggtattt atctgggtga tactttgatg acggaatggt ttagtaaaat tatggctatt
     3241 gtaattggaa tctttctaca tattagcaca actattttgt ttgaaagtga tagcaaccat
     3301 cgttttaact ttattaagat ggcagttata cttgccggag ttggtctttc attgatggta
     3361 taaaataaaa tcctcctgca atttattaca agaggatttt ccactaaata aaccaaatta
     3421 aattatgaaa tttttatttc acgcaacggt tttggtcttg cctcatcttt tttagggatt
     3481 accaaagtca ttacaccgtt gtcataggct gctgaaattt tttcactgtc agcggtttcc
     3541 ggcaaagtga atgtgcgctt aaagcttgca tacgaaaatt ctttgcgggt gtacttttcg
     3601 ttctgtgttg cttgttcttc tttcttctcc gcactgatgt tcaacatgtc attctcaatg
     3661 ttaatcttga aatcatcttt gctcaaaccg ggagctgcaa gttcaagttt aaattcgtga
     3721 tcagattcct taacgtttac agaaggactg aatgatttcg tgatgttgtt gctaaccaca
     3781 ccttcaaaga agtcgttgaa caaatcactg aataatggtg agcggtccat cattcttgat
     3841 gccggttcgt ttttaaattt aatgagtgtc attttgtttt tgttttaatt gttaatgtac
     3901 aaagcatata tcaaattcaa ttccattaac ttttttatga caaaaccggt ataattgagt
     3961 gagtttgtaa gtcatattgt ctatataaca aagaaaatga cgacaatttt tccgattcat
     4021 ggaatggcgt attgctattt actttatatt aaattcttat atgaaattct cttaagcttg
     4081 ttaattattt aaagtgaaaa caatcaattc ataaaacaga attatacttt tgtgcctttt
     4141 atgacttttg aataatttat gaaagcatca cgatttttat tgataggatt aatcattgca
     4201 ggattaattg ctgttaagtt gatatttttc cctggaacta aaacttcaaa gtttggtggt
     4261 aaagcaaatg cagcagcagt tcctgttaca gggtttattg tacgttctca agttcttgaa
     4321 agtaatttaa aggcgaatgg aacaatcaat gcttatgaac agacagactt gcgcgcagag
     4381 atttccgggc gcattgttgc aatattattt aaagatgatc agccggttaa gaagggtcaa
     4441 ctgttgatac agatttataa tgatgatttg aaagcaaatc tttcaaaaag tacagctgtt
     4501 ttaaagcaaa cacaggacag gctaaagcga caggaaaagc tattggaaat aggtggggtt
     4561 agcactgaag aggtagaaga aacacaaacc gaagcggcca ctcagaaggc tgaaatagat
     4621 tatttaaatt ttcagattac taaaacagaa atatatgcac ctttcgatgg tattgttgga
     4681 atcagagata taagtattgg caactacgta aactcgtcag ccgttattgc tacagtttat
     4741 caggtcaacc cattaaaaat agactttact gttccggaga agtatctacc ttatatcagt
     4801 aagggaatga aaatcagatt tactacagat gtaaataaga aggaatatga aggtgagata
     4861 gcagcaatca atccaatgat agacccttca acaaggatgg ttcgccttcg tgcttacact
     4921 tcaaacagcg atcatacatt gacacctggc tcatttgcgc atattcaggt tgattttaaa
     4981 atcacagaaa atgcaatact gattcctact caggctgtaa ttccaatttt aaaaggtaag
     5041 aaagtatttg taagcaaagg cggtaaggca gaagaaaaga taattgagac aggggaacgt
     5101 actgcagata tgatagaagt tactacagga ctgacggtgg gtgatactgt attgacaacg
     5161 ggaattttgc aggttagaaa aggaagtgat attaaattca gcgaggtaaa gtaggcgtat
     5221 ggatctgagt gaaataagtg taaaacgccc cgttcttgcc atagttatgt ccttagtgat
     5281 actgttattc ggtatcatca gttatacttt tcttggtgtc agagagtatc catctattga
     5341 tccgccaatt atcaatgtaa gaacatcgta tcccggtgcc aatgcagata ttattgagtc
     5401 gcagattaca gaaccgcttg aaaaagtaat taacggtatt cagggtattc gttcgatttc
     5461 ttcaacaagc agtcagggaa caagtaatat tacggttgaa tttaatttga attctgatct
     5521 cgaagcagca gccaatgacg taagagataa agtgtcacaa gctgttcgta ctttaccacg
     5581 agacattgat ggattgccag tcgtaaccaa atccgatgct aatgccgatg ccatcatatc
     5641 gctcacggtt cagagtaata cgcggaatac acttgattta agtgactatg cagagaatgt
     5701 aatcggtcag cagctaatca ccattcccgg agtaagtact ttacagatat ggggacagcg
     5761 taaatacgca atgcgtttaa gaatttatcc agataagctt gcagcatata aacttacagt
     5821 tcaggatatt caaaatgcat taaaccgtga aaatgtagaa ttgccggctg gaaaaattgc
     5881 aggtgacaat actgaacttg ttgtacgtac tacaggacag cttcatagtg aagacgattt
     5941 caataatatg attatcgtta ataatggtga tcaacttatt cgctttagag atataggtct
     6001 tgcagaatta gggccggaga atgaagaaac tattctaagg gaatcaggta ttccaatggt
     6061 aggtattgct cttgtgccac aaccgggaag caattacatt gaaattgccg atgaatttta
     6121 taaacggtta gaaaaaataa aagttgacct gccatctgat attaaaattg gaatagctct
     6181 tgacaataca acttttgtaa agaaatcaat taccgaggtt gaagaaacac ttttcattgc
     6241 aattaccctt gttgtaatca ttatctattt atttttccgt gattggcttg tagccattcg
     6301 tccattgata gatatacctg tctcgttggt tggagcaatg ttcattatgt acattgcagg
     6361 attttcaatt aatgtgttaa cgcttttagc catagtcctt gctacaggat tggtggtgga
     6421 cgatggtatt gtggttactg aaaatattta taaaaaaatt gaagccggac tttcacccaa
     6481 caaagcagca attgaaggaa ccaaagaaat attttttgct gtagtcagca cctctgtcac
     6541 attagcagcg gtttttcttc ctgtagtttt tatggaagga ttcgttgggc gactattcag
     6601 ggagtttggt gtggtcattg caggggcagt gcttatttct gcttttgtgt cgttaacctt
     6661 aacgccaatg ctaaatgcgc gcttagtgag aaaggataag aagcattcaa aattttatga
     6721 aaagacagaa ccgttttttc aaaggatgaa tcaggcttat gaaaattcgc taaagtggtt
     6781 tatggaagca agatggcgcg catggacaat tttaggctct gctattgtga taatttttat
     6841 tttaggcaga agtttgccaa gtgaactttc accattagaa gacagaaact ggctgcgact
     6901 attgattaca gcacctgagg gagcttctta tgaatataca gataatttta tcgtgaaaat
     6961 ttctgatgtc attgaagact ctatacctga gaagaatgga ttgcttacag taactgcacc
     7021 aggctttacc ggagctggag cagtaaattc aggctttgca cgcattgtac ttacaacacc
     7081 tgacaagcgt gagcgttcgc aacaagaaat tgcagatcaa ctttcgggaa ttactcgtaa
     7141 gatgactgat gcaagagttt tcgttcttca ggaacaatca ttgtccgcag gtggatcaag
     7201 aagcgcatta cctgttcaat ttgttctgca aaatcaggaa ttcgataagt taagagatgt
     7261 tgtaccagcg tttttagata gagcaaataa aagcccagta tttcagggtg tggatgtcaa
     7321 tctgaaattc aacaaacctg aactgaatgt ttccattgac agagctaagg caaatgcatt
     7381 aggagtgtct gttgatgata ttgcacaaac acttcaatta gctttcagcg gacaacgatt
     7441 cggatatttt atcatgcaag gaaaacaata tcaggtcatc ggccaggtag atcgtgaaaa
     7501 tcgtgatgtc ccttacgacc tttctaaact ttatgtaaaa acaaatagtg gaggtcttgt
     7561 tcaacttgac aatttaattg aagtaaatga aatcagcagt ccaccacagc tgtatcattt
     7621 taatcgttat cagtcagcaa ccatatcagc aggacttgct tcgggctaca caattggtga
     7681 tggaattaat gaaatgaaaa atattggtga cagtttgctt gatgaaagtt tttcgactga
     7741 tttaaccggt ccgtcacgtg actttgccga gagtagttca aatgtttggt ttgcttttgt
     7801 gcttgcaatt attttaattt atttggtgtt ggcagcacag ttcgaaagtt ttgttgaccc
     7861 atttattatc atgattacag ttcccctagc tatttgtggt gcactgattt ccttgtggta
     7921 ttttaatcag acattaaata ttttcagtca gattggtatt atcatgctca taggccttgt
     7981 taccaaaaat ggaattctga tagttgagtt tattaatcag cttcgtgaaa aaggagttga
     8041 gcgatcagag gcaattattc atggttctgt atcgcgtttc cggccaattt taatgacaag
     8101 tcttgcaact tcattaggag cactgcccat tgcattagca ttaggagcag gagcaaagag
     8161 ccgtatgtca atgggtattg taatagttgg aggtgtactt ttctctctga tatttacttt
     8221 gtatattatt cctgcaatgt attcagtttt ttcacgaaaa aaagaaaagg taatttatga
     8281 ttaaggtacg attaacagta ttgatttgtt ttttcttcgt tcaggcacaa agtcagcagg
     8341 tgtttacttt gcaacaggca ttagaaaagg cgcttaatga taatttttca attcgtattg
     8401 cccgtaatca aagcacaatt ctcaataaca atgcaacatt aggtaatgca ggaatgttac
     8461 cccaggtaaa tttgaatggc agctatgtta aggcaaataa caatgtggtt caggagttaa
     8521 gtaatggtac ttctaccgaa aagcagaatg caggccagtc aactattgca ggcagtgcag
     8581 tattggagtg ggttttattt gatgggctta ctatgttttc tgaatataat aaaaattctg
     8641 cactctcaaa acaaggaaaa gagcagctga aggcaaacat tgaagatgtg attgagaatg
     8701 taagcaaaac ttattttact gttgtaaaag aaaagttgtt gctgtcaagc attaatgaaa
     8761 cattgaaatt ttatgcagac agacttcaga tagcagatag taaatataaa attggttcag
     8821 cctctaagct ggattatctg cagtcgaaag tagattataa tgcaatgctt tcgcagaagt
     8881 tgaataaaga aacagatgtc aataatgcaa tgattgagtt aaaacggtta ttgcaatttt
     8941 ctgccgacac catgcttcag acagaagaca caatcacatt tatggctatg tcttcaatga
     9001 gtgatttgct gaatggctta caacaaaaca cagcaatgca agtgagcaag ttgaatgtag
     9061 aagtaaatcg tttgcagctg aattctatga agggattgcg tatgcctgta attaccggcc
     9121 tggcaggtta ttattacaat gaaagccgca acgatgccgg attgttttct ttgaacagaa
     9181 ccaatggccc acagtttgga attactgctc gttataattt atttaacggt ttcaataatt
     9241 cgagacaaat tagtaattca aaattgaatt atattaattc tcaaattgaa tatcagcaga
     9301 cacgtaccaa ccaaattgct gatttcaata atacttggaa tgcatttcag aaagcacagg
     9361 agatacttaa tttagaaatt gagaatactg ctgctgcgcg tgaaaacgca agtgtatatc
     9421 ttgccagcta tcgtttaggt agtgcaaatc ttttgcaatt aaatcaatcg caggaaagtt
     9481 tatatgatgc cttagtacgt ttggttaatg cacagtataa taacaaaact gctgaaatcc
     9541 atttactgcg tttggcaggt aaattaatta aataaataga caaaaactta atccggcttc
     9601 tttgcaatta ctgtatagaa tgtcgggtta aaagcttcaa aaattccaat accaccttta
     9661 atatttgtag gaaagactgc tggcgaacca aaaggactgc cactgcttcc ggcctgtgat
     9721 tcggcctgac gccaaaactt atagctctct tctgttatgg tacagaattt taccattatg
     9781 gtgtcaccgg tcttataaaa tcctgcttct tcattctcat cttcagacgc tgtactgttt
     9841 ggcaaatgcc cacggttgta agcaaaatca aatgctttgc cattaataaa tttatcttca
     9901 aacactgagc ctgatggtgc aataaacatt ttatccttgt ttaatcgttt ggcaaaccac
     9961 cgatagcaat ttcctaaagt atcaggatct tttaaatgtg cccatacata acctaatgaa
    10021 tctttccctt cctgaatttt aaaccataca ctatctaatg gaacggcagg gagcattttg
    10081 gtttcagcag taagcaattc attgtcgggc gtaatgatgc gtagtgaata gtttttacct
    10141 gtttgtccaa taatttttgg tgagaaatag aaataaccaa ttgacggatc aacctgtagg
    10201 agtgtgtctg taatgtcacc atcagaaata actatggtag catttttgac catgtattgt
    10261 tgtaatgctg tagaatcaac aggtgaaaaa taatctgctg ttttggaaag catgacatag
    10321 gcaggcaacc cattttcaat atagccttct acaacatatt gttgtttctg ttttgggaga
    10381 ttaactgtaa tatctttttc gcaagagatg aataatacta ttgcagaaaa gattaataca
    10441 agtagtttaa gatgatttat tttcatgtac atcagaattt aaagttataa gtaacagaag
    10501 gtatgatcgg aaacaatgaa acttgtttag cctgaatttt aaattcacct gtttctttat
    10561 caacttcatt attgaaataa ataaaatagg gattcattcg gctataaacg ttatagatgc
    10621 tgaaattcca gctcgactca aacctttttg tctttttcct gatataggtt gcagataaat
    10681 ccaagcgatg gtaggcagcc attctgtacg aatttctgtc tccataaaca tcataaattt
    10741 gtccgcttga catgttaggc aatccacttg aaacaacacc ttgcaaatct aatggcccga
    10801 acataaaata tcttgaaaca ggcagggtag ttgcattacc tgttgcgtaa acccatgtca
    10861 aaccaaatgt ccaacttttg cttgcattat atgacaccac aacagaaata tcatgtcgcc
    10921 tgtcatactt tgccggaaaa gtttttccat tcattatatc cggaaatttc ctgtcagtaa
    10981 agctaagtgt gtacccaacc catccattta atttcccttt tgctttttta ataaaaaatt
    11041 cagcaccata actccatcct ttaccaaaaa caaaattgtt atcggtatta tttttaacat
    11101 catcatcagc ttgtgcgcct tcgcgatatt caatttgatt acgcatggtt ttatagtaaa
    11161 cctctacgga tgtttcaaac atattctgat gaaagtttct gaaatatccg gctgcataca
    11221 accttccgtc ttgcggttta actctgtcac tgcttggaac ccaaacatct gttggcagtg
    11281 tgactgaagt aaatgatgct aagtgtacat attgataatt ttgagtgaat gaggctttta
    11341 ttgaagagtt tttgtctaat gaaaatttag cagaaatgcg tggctcaagt ccatgataga
    11401 atccaacagt ttcatttgtt ttgtaataaa tagtatcaac agtttcattg tcttcgttct
    11461 taacatagcg agtaaagggt cctgtaaaat cgaatagtga ataacgcaag ccggcactta
    11521 ccgaaaattt atcactaata gtccagtcat cattaatgta agcagctgct tcatgagcat
    11581 agagtctgtt tactcctcca agatcaaact cagttgaatc ctgactggcc tctgcacttg
    11641 ttggagtaaa gcgatgataa gtgtagttta ctccaaactt aacattgtgt gtgataatcg
    11701 gaaaccagtt aaagtcaatt tttgcattgt aatctctgat gcctgaaaaa atattaaaac
    11761 taaaactttc ctgcttggcg ctgaaagcaa agttgtaatc agaaaatatg attgaagtat
    11821 tcacaaataa tttatcactg aaaaggtgat tccatcgtaa aacagcagta gcgttacccc
    11881 acgggattct gaacttaaat cctgtgtcgg cactcttaaa gttaaacaca tctctgccaa
    11941 aatagccact taaaaaaagt ctgtctttat ctgaaagttg ataatttatt tttgcattca
    12001 aatcatagaa ataataggtg ttgttagcag catttgaacc tttttttaca aatggaggac
    12061 gttgaaaaag gtcaataaat gtgcgccttc cagatacgat gaatgatgat ttttcttttt
    12121 gaatagggcc ttgcaacgtc agtcgtgagg caattggtcc caatccacct tcagccttaa
    12181 actctttgtt gtttccttct tttagcgaaa tatctaatac agatgcaagt cgtcctccat
    12241 aatttgcagg cataccacct ttaatcaaag aaacattatt gattgcatcg gaattaaaca
    12301 ctgaaaaaaa accgaataaa tgcgaggcat tatatactaa tgcttcatcc aataaaataa
    12361 gattttgatc cggaccacca ccacgaacat aaaatcctgt gttgccttca ccggcagatt
    12421 gcactccggg cagcaattga atggttttta aaatatcaac ctcacccata aaggcaggca
    12481 gtgtttttat ctgttccaca tcaagctgaa cagttcccat ttgtgtgctg ttaacattct
    12541 ggtcttcttt cttagcagta ataacaactt cttgagtttc tatgtatttg ttacgaaggt
    12601 taatatttat cctgacatct ttggtaatat gcattttttg catatagtca ctataaccaa
    12661 caaatgagct cattaatgta atgctgtcgg cagcatcaac agtaatggaa taatagccgt
    12721 aggtgtttgt ctgaacacct ttcatcgtct cgcggagata cacattagcg ccaattaatg
    12781 cttcacctgt tgctgcatct ttaacataac cactaatagt aaatcgggtt ggtttgtcct
    12841 gggcgtttaa aatttgaatt tgtaaaaaaa gaagtaaaaa gagagaatat tttctgatca
    12901 taaattgatt gtacggggct gcaaacttat catttcattc tgaaattttt actcaataca
    12961 ttgaaatgtt aacataaatt tatggagctt agatttatgg tatagaaaag tgtttttgtt
    13021 gtaaatttgt taataagtga gttactcctt acaatcataa cagatttttt tatttaaaat
    13081 tgttgaccaa taatataatg atgaaaaagt tacatgtttc cttcttagta tttttaatat
    13141 tcaatcttca aggtgcattt gctcaggata tgagtaaaat aaatttttca cttgctaaaa
    13201 agatatccgg gccgtatccg gcaacacgcc ctttagcatt atttgtgcag ggagatgtag
    13261 gtgttattca gaattatatt caacagaata aaggtgaggt aaagtttgtt gcaggagata
    13321 ttgtttcatt ggtcatgcca ttaggtgctg ttaatggatt gatagcactt ccaagtgtta
    13381 aagcagttga agataacgga atgcaacttg tagcattaga tgattctatg cgtgtgcgca
    13441 acaacatcaa tccggtacag aatggcatgg cgcctttgcc gcaaggctac gatggcagtg
    13501 gcgtagtaat gggctttatt gattccggaa ttgatttttc tcatcctgat ttcaaacatg
    13561 ctaacggcag tacacgtgtg ttgtatatct gggatcataa tttatcagta ggtgctgcac
    13621 ccatgccgta taattacgga acagaattta cagcagcgga tattgatgca ggcaatgctt
    13681 ctgctcatgt tgataatagc aatgggcatg gcacacacgt cacaggtatg ggggcaggta
    13741 atggtcttgc attgaatgat cataaaggtg tagcgcctgc tgctgatatt atttctgttt
    13801 gtgtaaactg gaaccttaca gacaatgatt ggttaacaac agttgctgat gctgtaaatt
    13861 atatttattc aaaggcgtat gctctcaata agccttgtgt aattaatatc agtgcaggaa
    13921 cttattatgg ttcgcatgat ggaaaagatt tgcaggcaca ggctatctcg aatctcatct
    13981 cacagcggaa tggtcgttca cttgtttgtg cagccggtaa tgccggaaat ttgccaattc
    14041 atgttcagca tttgcatact gctgccaatg atacatcgtt tacatggttt
//
LOCUS       DAOQNP010000078        13513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1614_length_13513_cov_8.389954, whole genome shotgun sequence.
ACCESSION   DAOQNP010000078 DAOQNP010000000
VERSION     DAOQNP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1614_length_13513_cov_8.389954"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2051
                     /locus_tag="PKY09_10230"
     CDS             <1..2051
                     /locus_tag="PKY09_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB13272.1"
                     /translation="LLGYETFQKVILVGENNFFQIHLIPLSKQLQSVVVSAGKFEQKL
                     EEVTVTMETIKPKSFETTGDNNVETALEHVTGLNVNDGQANIRGGSGFSYGAGSRVLV
                     LVDDMPMLSADANDAKWSFLPIENLEQIEIIKGASSALYGSSAMNGVINFRTGYAKSD
                     PVTKIVQYNGIYDSPRRKEIKWWGKETQMLSGANMFHSQKFGQWDFVAGGHYLLDEGF
                     RKGETEKRARVNINTRYRFPKIEGLSAGVNANYMESFGGLFIIWNDDTTGAYIPSGGV
                     DDSTSTLSNYHTVRYNIDPFITYHSKKGFSTKWRSRYFRTLNYNDTDQEAFADLYYSE
                     VQLQQRFAKWNLVLTGGGVITDGGVRSDLYGSRKTYNQALYAQADKKFFDRLNLSFGW
                     RWEQFRFRYGKAESKPVIRAGANLRLLRATYLRSSYGQGYRFPSIAEKYVRTRVGSIV
                     IYPNDSIFSESGWSAEIGLRQLFAAGKRFSGYVDASVFRSQYKNMLEFAFGSYGDPFK
                     DPLFGLGFKSLNIGNTRIDGMEFEMGIQLTILKKLNLQLGGGYTYIDPRLTDFDSTKA
                     ATGTVSYNFLKYRYRNLFKGFGSIEYGKFSFSMNCRYNSFMENIDVVFNELLPGVKRY
                     RANHHGGDWVYDANVSYKVNPQMALSIISRNLFNHEYSGIPSQLEAPRMWLFQIVITP
                     "
     gene            complement(2074..2745)
                     /locus_tag="PKY09_10235"
     CDS             complement(2074..2745)
                     /locus_tag="PKY09_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB13273.1"
                     /translation="MNKSLTDKNLKVIIVDDNKNIRESVSLLLQDTPTLQLVAAYDSG
                     VECIKHLEEIMPDIILMDIDMPKMSGIETVKIIRKSFPGLPILMLTGFEDDDKVFNSI
                     CAGANGYILKNASMELLINQIHEAYLGGAPMTPVIARKVLNMFSASFMVNHQQNENYN
                     LSKREKEVLELLVNGKSYKVIATELAISYETVHSHIKKIYHKLHVSSIGEAISKTIKQ
                     KILRK"
     gene            complement(2803..5871)
                     /locus_tag="PKY09_10240"
     CDS             complement(2803..5871)
                     /locus_tag="PKY09_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB13274.1"
                     /translation="MYRLTISILLFLFGYFHTHADSLVYNLRSYNTTSGLSDNNVTCI
                     VKDHYGFIWVGTKDGLNRFDGREFQVFQYQARSNSVCGNNISCLEITDDSLLWIGTAS
                     NGFCSYDYRTNQFKSFTSQNSKLRVDNINDLVFDRRRNCLWIALNNGGLQQFDLSKMQ
                     INPKIFSYNTYYAVAVKDSLAFFAGITESLKTLNYLGKAIINADGNGQTINTIYIDTD
                     SRIWCGAWDNALHLFNQQGKRLNSYVFDGTNKLKSIGEEIISITGDDENIWCGTKSSG
                     ILIFNKSSKTFLKNISFTQKINTRVNDLYRDPQNRIWIATENGLLVYDSQLNLFKTEH
                     LPVPKNVTSCKVNDRIITDNGLDLVISQCGLFYRYSKNESYKFKDIYYKNEKQELYSI
                     IQNKNGDIFIGSNRTVFKFNTKDLSCHPIELHKNLSNLFFYFASASPINSLASININN
                     SELLLASFYGYFITLIDLKKHNLFHLSQDSASTIAGLDNLSRKLFVDSKNNIWICGKS
                     KGISFLDLNNTSPLNNYAPFYDKIISWHVNPANWQKNINNTALKITNVYDIVENNDGT
                     YWLTTQGTGLVHFDKNNKNNPFAMTLSDITSMQGLTQTGDSLLWIITSSGILKFHTKT
                     KRFKLFDSNNGIQQNIAGYFFKKNKFLPDEVSVGFNGGFISFNPRQFSSPKEKCEIQF
                     TKLWVMDKPADTLLFKNIKLNYNQNFLKVYVSSNVFTTNEQITYYYKLSGIDLDWRSN
                     EQNPLITYTNLPPGCYTLKVKTVTIDNIESNIKSLSIVITPPFTKTIWFYLIIACCVA
                     IIVYMIHRYRINQLLQLQEMRNNIARDLHDDIGSTLGSIHLYSQIADKKLSQSNTDDT
                     KNILEKIRSSSKEIIEKTSEAVWVVKASNDNIANLTLRIESYAASVLGDAGIDFSLQS
                     NSHQNIKLSMHQRKWLFLIFKEAIHNIVKHSHCTKVEIALNNYNRVIEMIIKDNGIGF
                     NSTADRNCIKNGNGGNGITNMASRAADMNATFNIISAQGTGTKITISLKH"
     gene            complement(6021..9230)
                     /locus_tag="PKY09_10245"
     CDS             complement(6021..9230)
                     /locus_tag="PKY09_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13275.1"
                     /translation="MRRAVNCILFLAFLLCTASSTAQLTLAEWNFPTNPDNAIVDISI
                     PINNTKTLYTVGGTAAVAYGTNTGATTQSAWTTGWDNGSGTKWWEIEVNTTGYFNLEL
                     SSKQRSSATGPKNFAVDYKIGIAGTWTALAGAGAIIVADNFTSGVITDIPLPVACENQ
                     PSVFFRWKMTSNTSVNNSTVASGGSSRIDDVNIKANDSQNHYRSITSGAWNVLSTWQS
                     SPDLITWSAAQILPNYYSKTITIRSGHTVTISANVRVDETTIANGGTVNYNSRVLTIN
                     NGTGVDLQVNGTFTDGSSSSAVWLAGATWALGASGTYVKTENTNAVNWQNNYQGGIAT
                     IPATASWVLNKTPSSVTAPSLTTLNMVYPNLTIMNTSGGLWTTSIASTFTGSTASATI
                     KGNFDIGGVLAPLLGTVDFLNDNSFATPIQVQGNVLIRGGCNLRNFGTGFEIFGNMTV
                     NGSTSYGTSNARKYRFSGGNAQSISGSAPLTSLQIFQLEMNKSANSLTLLKSIKVDNN
                     LNLITGIINSTTPNILIVENGATATGASNSSFVNGPVQKNGSQAFVFPVGKNSNYRSI
                     AIGNDASAAAFWTENFNTGAGWSLANVQGPEGADPNPFVISDAEGGGIAPGGCGAANN
                     GNNTLHVTSVFNPNGGAAYDAGGLCGFLYCPLTNRMAQSPPINCTGKSTISVGFNYIE
                     FGDGTNDNATLWYFDGATWSQLFDLPKTTCCGGACNGFRQGKWTAFSITLPASANNNA
                     NVRIGLKWQNNDDGAGTDPSFAIDDIYLSASSTSQFVAEYHRANPQVIIGNIRDASLD
                     HISQCEYWTLNQTLGTSSRNVTLSWDGNSCGVTLLSDLRVARWRSLGSIWNNLGNGGT
                     TGTTTAGTIVTTAPDNIFGPYTLSSASSENPLPVELTYFNATLQNKEVKLQWTTASEF
                     NSDYFEIERANSNLTFEPLMRVPAAGFSNSLINYNTTDTKPLSNESFYRLRQVDFNGD
                     FQLSKTVKINIQNQLPFQIILASYLNNGQLIFKITENMQPTIAVEIYDTHGSLILQQS
                     FNSSESLYQIQTNNLANGIYLLKVISDKSVATKKFSVQK"
     gene            complement(9416..11146)
                     /locus_tag="PKY09_10250"
     CDS             complement(9416..11146)
                     /locus_tag="PKY09_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13276.1"
                     /translation="MKKTLLVIFCLTASTLYAQTPIFDWAFGLGGTYYDKGIDVATDS
                     AGNVYALGSFANTVDFDPGSNVLNLTSNNNSDDVYIAKYNAAKDLLWAHVFGGTGNET
                     PIAMALDASGNCYLTGRFSGTVDFDPGAGTANITASGAFDVFIVKLSPSGIYLWAKNF
                     GGTFGTFVDEISVDDAGSVYTAGTFQTTTDFDPSPAVFNLVSTGGQDPYLAKLDAAGN
                     FVWALNTGFNISKLYCDINNSLFIAGGFNDTTDFDLSSSQTLNIVPSGNYDIYISKYD
                     TAANLIWVKTMGSTGSSGGVLGITTDMAGNIIATGTFSGTLDFDPGTNTVNLTSSGSG
                     STLSDMYFLKLDAAANFVWVKKIGGNNLDYPLDVATDNAGSIYLTGVFKSSVVDFDPD
                     TTVTFNLNGTMGSYDAFVLILSATGNFIYAYEIGGPSNGDDYGNGICVNPSGSYYVTG
                     TFYGPIDFNPGAGTTTLNDNGNTDAFIQKMNFSLVGLVDTDEENYFSVYPNPANQIVT
                     INLPSVKNQSSEVKVFDAIGKEILSTRVAGGNSTIDVSKLDKGIYFIQLTANTENQKH
                     LQVKKLIVND"
     gene            complement(11594..>13513)
                     /locus_tag="PKY09_10255"
     CDS             complement(11594..>13513)
                     /locus_tag="PKY09_10255"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNB13277.1"
                     /translation="ANGAPFKATTTMYSTQAAWQAAMTINPNSDVNSQFADPHFVNNA
                     NNLHATSASTSLDGTATTPPAYITTDIDCQTRTAPHDIGFDDFSLAVNVYYADTDGDT
                     FGDLNNSITSPSPTPPPGYVTNSTDCDDNNAAVNPGATEVCNGIDDNCNGQIDEGVTT
                     TFYADTDGDGFGDAAVTTQACTAPVGYVSNNTDCDDNNNTIYPGATEVCGNGIDDDCD
                     GQVDEGCGSNTWYADADADTYGDLNNFVVDNNPTPPAGYVADSTDCDDNNAAVNPGAT
                     EICGNAIDDNCNGQVDENSPSAAIAGADTAVCSVNSTTLGATAPLVGSGSWSIVSGVG
                     GSIAQPNNPNSTFSGQYNKPYVLEWTTSNGGCVFSDQVTVIFVNTNYSPGRIAGFSGQ
                     TSAVCGLTQTYAAIPNHPYATYSWSGYPAGTTVNLQSGNQITLTYPSSGFNYFQLKVT
                     STVCGVQTPIRSVAMKGAPSVKVANGPAIVCANATGIVYNVDLLAGVTYNWSVPQGAT
                     IVNGQGTNEITVDWGNSGGLVLCNIWNSCGSSTAKKSVTTNCRSAQQTLTESNSTLIS
                     IFPNPSSGVFNLHADNQLENVQITVTDLQGRTLINTLFTGNETTVDLSNYTQGVYMLQ
                     LHSNKEVQTYKLIKD"
BASE COUNT         4053 a         2395 c         2688 g         4377 t
ORIGIN      
        1 aattgcttgg ctacgaaact ttccagaaag taatactcgt tggcgaaaat aacttttttc
       61 aaatccatct cataccttta agtaaacaat tacagtcggt agtagtgagt gccggtaagt
      121 ttgagcaaaa actggaagag gttacggtaa caatggagac cataaaacct aaaagctttg
      181 aaacaacagg tgataataat gttgaaacag cacttgagca tgtaaccgga ttgaatgtaa
      241 atgatggaca ggcaaatatt cgtggaggca gcgggtttag ttatggcgct ggtagcaggg
      301 ttctggtgtt ggttgatgat atgcccatgt tgagtgcaga tgccaatgat gcaaaatgga
      361 gttttctgcc aatagaaaat ctggaacaga tagaaattat taaaggagca tcatcagcgc
      421 tttatggttc ttcagcaatg aatggtgtca taaatttcag aactggatat gctaaatcgg
      481 atcctgtaac aaaaattgtt caatataatg gaatttatga ttcaccccga agaaaagaga
      541 ttaagtggtg gggtaaagaa acacagatgt tgagtggtgc aaatatgttt cattcacaaa
      601 agtttggtca gtgggatttt gttgcaggag ggcactattt acttgatgaa ggttttagaa
      661 aaggcgaaac agagaagcgc gcaagagtta atataaatac acgatacaga tttcctaaaa
      721 tcgaaggact atcagcaggt gtaaatgcca attatatgga atcattcggt ggtcttttca
      781 ttatttggaa cgatgatact accggtgctt atattccaag tggaggcgtt gatgattcaa
      841 catctacttt gagcaattat cacaccgtaa gatataatat tgaccccttt attacttatc
      901 acagcaagaa gggttttagt acaaagtggc gctctcgata tttcaggaca ttaaattata
      961 atgataccga tcaggaagca tttgccgatt tatattattc tgaagttcag ctgcaacagc
     1021 gttttgcaaa atggaatctg gtgcttacag gtggaggtgt aattactgat ggtggagtac
     1081 gcagcgattt atatggtagc aggaaaacat ataatcaggc attatatgcg caggcagata
     1141 aaaagttttt tgacaggctt aatttgtctt tcgggtggcg ttgggagcag tttcggtttc
     1201 gctatggcaa ggcagaatcc aagccggtaa tcagagcagg agccaatctt cgtttattac
     1261 gtgctacgta tttgcgcagt agctatggac aaggataccg gtttccttca atagccgaaa
     1321 agtatgtgag aaccagagtt ggatcaattg tgatatatcc aaacgattcc attttttctg
     1381 aaagtggatg gagtgcagag attgggttaa gacaactgtt tgccgcagga aaaagatttt
     1441 cgggctatgt agatgcatcg gtattcagaa gtcagtataa aaacatgctt gagtttgctt
     1501 tcggcagtta tggcgacccg tttaaagatc ctctttttgg tctcggtttt aaatcattga
     1561 acattggtaa tacacgtatt gatggaatgg agtttgaaat gggcattcag ttgacaatac
     1621 taaagaagtt gaatcttcag ttaggaggag gatatacata cattgaccca agacttaccg
     1681 attttgattc tacaaaggcg gcaacaggaa ctgtgagtta taatttttta aaataccgat
     1741 accggaattt gtttaaaggt ttcggaagta ttgaatatgg taaattttcg ttttccatga
     1801 attgcagata caatagtttt atggaaaata ttgatgttgt gtttaacgaa ttattacctg
     1861 gtgtgaaacg atatcgggct aatcatcatg gtggcgattg ggtatatgat gccaatgtca
     1921 gctataaagt caacccgcag atggcacttt ctattatttc gcgcaatttg ttcaatcatg
     1981 agtactcggg tattccttca cagttagaag caccgcgaat gtggctgttt caaatcgtaa
     2041 ttactccttg atggcaacag caacagtatt atgttatttg cgaagtattt tttgttttat
     2101 ggtctttgat atcgcttcac caatagaact gacatgaagc ttgtgataaa ttttcttaat
     2161 atgactgtga acagtttcat agctaatcgc taattcagtt gcaatcactt tataggattt
     2221 gccattgacc aaaagctcca atacttcttt ttcacgttta ctgaggttat agttttcatt
     2281 ttgctgatga ttgaccataa aagaggctga aaacatgttt aatacttttc tggctataac
     2341 aggagtcata ggcgccccac ctaaataggc ttcatggatt tgattgatga gtaattccat
     2401 gctagcattt tttagaatat aaccattagc accggcacaa atcgaattaa aaaccttgtc
     2461 atcatcttca aatccggtta gcatcagaat aggcaatccg gggaaagact tacggatgat
     2521 ttttacagtc tcaataccgg acattttagg catatcaata tccataagga taatatcagg
     2581 cataatttct tcaagatgtt taatgcattc cactccagag tcgtatgcag ccactaattg
     2641 taatgtgggt gtgtcctgta agagtaatga tacagattca cgaatatttt tattgtcatc
     2701 aacaataata actttcagat tcttgtcggt taaagattta ttcacagtac aaatctgagt
     2761 cttttttttt gccattgcaa taaccaatta tggtgagttt aattaatgtt ttagcgaaat
     2821 agtgatttta gtaccggtgc cctgtgcact tatgatgtta aatgtagcat tcatatcggc
     2881 tgctctggaa gccatattag taattccatt accgccatta ccatttttta tacaattcct
     2941 atcagccgta ctgttaaacc caataccatt atctttaata atcatttcta ttactctgtt
     3001 atagttgttt aaagcgattt cgacttttgt acagtgggaa tgttttacaa tattatggat
     3061 agcttctttg aaaatcagaa ataaccattt cctttggtgc atactgagtt ttatattttg
     3121 atggctgttt gattgaaggc taaaatcaat accagcatcg ccaagaacac tggctgcata
     3181 gctttctatt cttagggtta ggttagcaat gttatcgtta gaagctttaa ctacccatac
     3241 agcctcactg gttttttcaa ttatttcttt agaagagctc cttatttttt ccaggatatt
     3301 tttggtatca tcagtattac tctgactgag ttttttatct gcaatctggc tataaagatg
     3361 aatactgcct aatgtgctgc caatatcatc atgcaaatca cgtgcaatgt tattgcgcat
     3421 ttcttgtaat tgcagtagtt gattaatgcg gtagcggtga atcatataaa caatgattgc
     3481 tacacagcag gcaatgatga gataaaacca gattgttttt gtgaagggag gtgtaatgac
     3541 aatagataga cttttaatat tgctttcaat attgtcaatg gtaaccgtct taacttttaa
     3601 tgtatagcaa ccgggaggca aattggtata ggtaattagg ggattctgct cattgctacg
     3661 ccagtcaagg tcaataccgg aaagcttata gtaataagta atttgttcat tggtggtaaa
     3721 aacattggat gagacatata cttttaaaaa attttgattg taattgagtt tgatattttt
     3781 aaaaagcaat gtgtctgcag gtttgtccat aacccaaagt tttgtgaatt gaatttcaca
     3841 tttttctttt ggagaagaga actgtctggg attaaaacta atgaacccac cattaaatcc
     3901 aacactgacc tcgtcaggta aaaatttatt ctttttaaaa aagtatccgg caatgttttg
     3961 ttgaattccg ttgttggaat caaaaagttt aaatcgcttg gttttggtat gaaatttcaa
     4021 aatgcctgaa gaagtgatta tccataataa agaatctccg gtttgtgtca gcccttgcat
     4081 ggaagtaata tctgataggg tcatcgcaaa tggattgttt ttattgttct tgtcgaaatg
     4141 taccaatcct gtaccttgag tcgttaacca gtaggtgcca tcattattct caacaatgtc
     4201 atacacattg gtgattttta aagcggtatt attaatgttt ttttgccagt tagcagggtt
     4261 tacatgccaa cttattattt tatcatagaa tggtgcatag ttatttaatg ggcttgtgtt
     4321 gtttaaatct aagaaagata tacctttcga tttgccacaa atccaaatgt tattcttaga
     4381 gtcaacaaaa agtttgcgac ttaaattgtc cagacccgct atggtagaag cactgtcttg
     4441 agacagatga aacaggttgt gttttttcag atctattagc gttataaaat atccgtaaaa
     4501 tgaggctagt aatagttctg aattatttat gtttatgctg gctagtgagt tgattggtga
     4561 agctgatgcg aaataaaaaa ataaattcga aagattctta tgcaactcaa ttgggtggca
     4621 tgataaatct tttgtgttga acttgaaaac agttctgtta cttccaataa aaatatcacc
     4681 atttttattt tgaatgatag aatacaattc ctgtttttcg tttttgtaat agatatcttt
     4741 aaatttatac gattcgtttt tagaatatct gtaaaacaag ccacattggg atattaccaa
     4801 atctaatccg ttgtctgtaa taatacgatc attgactttg caagatgtta cgttttttgg
     4861 cacaggaagg tgttcagttt taaacagatt taactgtgag tcgtaaacca acagaccatt
     4921 ttctgttgca atccatattc ggttttgagg atcacgatat agatcgttga ctcttgtatt
     4981 gattttttgt gtgaaagaaa tatttttcag aaaagtttta ctgcttttgt tgaagatcag
     5041 tattcctgag ctttttgtgc cacaccaaat gttttcatca tcaccggtaa ttgaaataat
     5101 ttcctcgcca atactcttta atttattcgt tccatcaaaa acataggagt tcaatctttt
     5161 gccttgttgg ttgaagagat gtaatgcatt atcccatgca ccacaccaaa ttcttgaatc
     5221 tgtgtcaata tatatggtgt taatggtttg tccattgcca tcagcattaa taattgcttt
     5281 tccaagataa ttaagcgttt ttaatgattc cgtaatgccg gcaaaaaagg caagagagtc
     5341 ttttactgca acggcatagt atgtattata agaaaatatt tttggattga tctgcatttt
     5401 tgacaagtca aattgttgaa gtccaccatt gttcaaagct atccaaaggc agtttcttct
     5461 tctgtcaaaa accaaatcat tgatgttatc aactcttagt ttgctgtttt gtgaggtaaa
     5521 acttttaaat tgattggttc gatagtcata actgcaaaaa ccatttgaag cagtaccaat
     5581 ccataagagc gaatcgtctg taatttcgag gcaggaaatg ttattaccac aaacagaatt
     5641 tgaccttgct tgatactgga atacctgaaa ttctcttcca tcaaaacgat tcaggccatc
     5701 cttggttcct acccaaatga aaccatagtg gtcttttact atacaggtaa cattattgtc
     5761 agacagtccg gatgttgtat tgtagcttct taaattatat actaaagaat cggcatgggt
     5821 atgaaagtat ccgaaaagga aaagcaatat tgatattgtc aatctataca ttcaatgccg
     5881 gcttggttta atgatgtgac caaaataata aaaaaaagtg gctttattga acggattggt
     5941 gaagtaaccg ttagtacaga tgaaggtaat atttttgctg gtgcagggga atagatgtgc
     6001 taaacagcga agaaaagagg ttatttctgt acactgaatt ttttagttgc tactgatttg
     6061 tcagaaatta ctttcagcaa atatattcca tttgctaaat tatttgtttg tatctgatag
     6121 agtgattcgc tactgttgaa agattgttgt agtatcaggg atccgtgagt atcgtagatt
     6181 tcaactgcaa ttgtcggttg catattttca gtaatcttaa aaataagttg tccattattg
     6241 aggtatgaag ccaatataat ttgaaaaggt agttgatttt gaatattaat tttaacggtt
     6301 ttgcttaatt gaaaatcgcc attaaaatca acttgtctaa ggcggtaaaa cgattcattg
     6361 cttagtggtt tggtatcagt tgtattataa tttatcagac tgtttgaaaa acctgctgcc
     6421 ggaactctca ttaacggttc aaaggtcagg tttgaatttg cacgctcaat ttcaaaatag
     6481 tcactattaa attctgatgc tgttgtccac tgcaatttta cttctttatt ttgcaaagtg
     6541 gcattgaaat aggtcagttc tacaggcaat ggattttcac tggaagcaga ggaaagtgta
     6601 taaggcccga aaatattatc aggtgctgtt gttacaatag tacctgctgt tgttgtgccg
     6661 gtagtgcctc cgttaccaag attattccag atgcttccta aactgcgcca acgcgcaaca
     6721 cgtaagtcgc tcagtaaagt aacaccacaa ctgttgccgt cccatgaaag agttacattg
     6781 cgcgatgaag tgccgagcgt ttggttgagt gtccaatatt cacattgact gatatgatct
     6841 aatgaggcat cacggatatt tccgataatt acctgaggat ttgctctgtg atattcagca
     6901 acaaattgac ttgtagaaga cgcactcaaa taaatgtcat caatagcgaa ggaaggatca
     6961 gtgccggctc catcatcatt attttgccat ttcaaaccga tacgtacgtt tgcgttgttg
     7021 tttgcacttg caggtagtgt aatagaaaat gcagtccact tcccttgtct gaatccatta
     7081 caggcaccac cacaacatgt tgtttttggc aaatcaaata attgactcca ggtggcacca
     7141 tcaaaatacc atagtgttgc attatcattt gtgccatctc caaactcaat ataattgaaa
     7201 ccaacactta tagttgattt tccggtacag ttgatagggg gagattgtgc cattctgttt
     7261 gttagcgggc agtagaggaa accacataag ccacctgcat cataagcagc gcctccgttt
     7321 ggattaaata cactggtgac atgtaaggtg ttgttgccat tgtttgctgc accacagcca
     7381 ccgggtgcta ttcctccacc ttctgcatcg cttattacaa aaggattggg gtctgcacct
     7441 tcaggtcctt gcacatttgc caagctccat cctgcaccgg tattaaaatt ttctgtccag
     7501 aaggcagcag ccgatgcatc attaccaatg gcaatagatc tatagttgga gtttttgcca
     7561 acagggaaaa caaatgcctg cgacccgttt ttttgcaccg gaccatttac gaaactcgaa
     7621 tttgatgcac ctgttgcagt tgcaccattt tcaactatta aaatattagg agttgtagaa
     7681 tttataatac ctgtaattaa gttcagatta ttgtcaacct ttatggattt taatagtgta
     7741 aggctgtttg cagacttatt catctctaac tgaaaaatct gtaatgaggt tagcggtgct
     7801 gaacctgaaa ttgattgtgc attgccaccg ctgaatcggt atttacgtgc attagatgta
     7861 ccataacttg tagatccatt tacggtcatg tttccgaaaa tttcaaatcc tgttccaaaa
     7921 tttctgagat tgcatcctcc acgtattaat acattgccct gaacctgtat aggagttgcg
     7981 aatgaattgt catttaaaaa atcaaccgta cctaaaaggg gagccaatac accaccaata
     8041 tcaaaatttc ctttgatagt tgctgatgct gttgaacctg taaatgttga tgcaatactg
     8101 gttgtccaca aaccacctga ggtgttcata atggtaagat taggataaac catgttaagt
     8161 gttgtcaatg atggagcagt taccgaactg ggagttttat tcagtaccca gctggcagtg
     8221 gcaggtatgg tggcaatgcc tccctgataa ttgttttgcc agttgacagc attagtgttt
     8281 tcggttttta cataagtgcc ggaagctccc aatgcccatg ttgcaccggc aagccatact
     8341 gcactgcttg aactaccatc tgtaaaagtt ccgtttacct gcaagtcaac cccggtgcca
     8401 ttattgatgg ttaatactct ggagttgtaa tttacagtgc caccattggc tattgtagtt
     8461 tcatctactc ttacattagc agaaatggta actgtatgac cacttcgaat ggtaattgtt
     8521 tttgaataat aattagggag aatctgtgca gccgaccaag taataaggtc cggtgatgat
     8581 tgccaggtgc tcaaaacatt ccatgcacct gaggttatgc ttcgataatg attttgtgaa
     8641 tcgttggctt tgatgtttac atcgtctatc cgtgatgatc cacccgaggc tacagtacta
     8701 ttatttacgc tggtgtttga tgtcattttc catctaaaaa aaactgatgg ttgattttcg
     8761 caagccaccg gtaatggaat atccgttata acacctgatg taaaattatc agctacaatg
     8821 atagctcctg caccggctaa tgctgtccat gtaccggcaa taccaatttt ataatcaaca
     8881 gcaaaatttt tcggcccggt agctgaacta cgttgcttgg aagaaagttc aaggttaaaa
     8941 tatcctgtgg tattcacttc tatttcccac catttggtgc cggagccatt atcccagcct
     9001 gttgtccatg cactttgtgt agtagctccg gtatttgttc cgtatgctac tgcagcagtg
     9061 ccacctacgg tgtagagggt ttttgtgtta ttgatgggaa ttgaaatatc aacaatggca
     9121 ttgtcaggat tagtgggaaa attccattct gccagtgtga gttgagcagt ggatgatgct
     9181 gtacacaaca aaaatgccaa gaacaatata caattgacgg ctcttctcat atattagtat
     9241 cgaacaatca aagttacatt gtttttcgga ttaaaacaat attttgaaaa aaattatgat
     9301 tgaatttctt aacagagaaa tttactaata aaaaaggctg ccatttaaga gcagcctttt
     9361 ttagtaatat tgggttagtt tgtgtttgat aagctaatag gtttgtactt cttatttagt
     9421 cgttaacaat tagctttttt acttgaaggt gtttttgatt ttcagtattg gctgtaagct
     9481 gaataaaata aattcccttg tctaatttac ttacgtcaat agttgagttt ccgcccgcta
     9541 ctcttgtact taatatttct tttccaattg catcaaaaac tttgacttca gaagattgat
     9601 tttttacaga cggcaaatta atagttacta tctggttggc gggattagga tatacagaaa
     9661 aataattttc ttcatctgta tcaactaaac ccacaaggct aaagttcatt ttttgtataa
     9721 aggcatcagt atttccatta tcattcaatg tggttgtgcc tgcaccggga ttaaaatcta
     9781 tggggccata aaaagttccg gtaacatagt agctgcctga aggatttaca caaataccat
     9841 taccataatc atcgccatta gatgggccgc caatttcata ggcataaatg aaatttcctg
     9901 ttgcagataa tataagcaca aatgcatcat agctgcccat ggtgccatta agattaaaag
     9961 ttactgttgt gtcagggtca aaatctacta ctgaagattt aaatacgcct gtaagatata
    10021 tactgccggc attatctgtc gctacatcaa gtggatagtc taagttattt ccaccaatct
    10081 ttttaaccca aacaaaattt gcagcagcat ctaactttaa gaagtacatg tctgaaagag
    10141 ttgatccgga tccacttgat gtaaggttga cggtgttggt tccagggtca aagtccaaag
    10201 tgccagagaa ggttcctgta gcgattatat tgccagccat atcggttgta attcctagta
    10261 cacctccact actacctgtg gaccccatgg tttttaccca aattaggttt gccgcagtgt
    10321 catattttga aatatatata tcataattac ccgaaggaac aatattcaat gtttgtgatg
    10381 aagataagtc aaaatcagtg gtgtcgttaa acccccctgc aatgaaaagg gagttgttta
    10441 tgtcgcagta tagtttggat atgttgaaac ctgtgtttaa tgcccaaaca aaattccctg
    10501 ctgcatccag cttagcaaga taaggatcct gccctcctgt tgaaactaag ttaaacactg
    10561 ctggtgaagg gtcaaagtca gtagttgttt ggaatgtacc ggcagtatat acactacctg
    10621 cgtcatctac tgaaatctca tcaacaaaag taccaaatgt tcctccgaag ttttttgccc
    10681 agaggtaaat tcctgatgga ctaagtttaa caataaaaac atcaaatgct ccagatgcgg
    10741 taatgttggc tgtgcctgct ccgggatcaa agtcaacggt gccactaaaa cgtccggtaa
    10801 ggtaacaatt gccagatgca tcaagcgcca ttgcaatagg agtttcattt ccggttccac
    10861 caaatacatg cgcccaaaga aggtcttttg ctgcattgta tttggcaatg tagacatcat
    10921 cactattatt attagatgtc agattcaaga catttgaccc tggatcaaaa tcaactgtgt
    10981 tggcaaaaga cccgagagca taaacattgc ctgcagaatc agttgctaca tcaatgcctt
    11041 tgtcataata ggtcccccca aggccaaaag cccagtcaaa tattggagtt tgtgcataaa
    11101 gtgttgatgc tgttaagcag aagataacaa gtagcgtttt tttcatctta tttgatttta
    11161 attggtgatt atttatttag tagcatagat ttcatttgat ttattttttg ctggtgataa
    11221 tggcggataa caaatattct tttgtatctt actgatgtga actcatgtcg gaatgacttg
    11281 taaatgcaat gaattaaaat aaaataccat tgttgtcaaa aatccatttc atattttttt
    11341 aattagcggt ttgtaagagt atattataaa atatatttta caaatctgag tcaattaaat
    11401 ttaaacagca atcaccaatt gtggtgataa atcaggttaa ttaccgaatt ttatagagtt
    11461 tttgtagcat atttactata accggtattt aaatttatat ttctcactag ttgagtaagt
    11521 gtaaaatcaa atctttaaac aaaaaaggct gccttttaag agcagccttt ttgtaataga
    11581 attacgaagt ttattaatcc tttatcagtt tataagtttg tacttctttg tttgaatgta
    11641 attgtaacat ataaacacct tgagtgtagt ttgataaatc aacagtagtt tcattaccgg
    11701 taaacaaagt gttaatcaat gtacgacctt gtaaatctgt tactgttatc tgaacattct
    11761 ctagttgatt gtcagcatgc agattaaata ctcctgaaga aggattaggg aagatgctga
    11821 ttaacgtact gttactctca gttaaagttt gctgagcact acgacaattt gtagtcacag
    11881 attttttggc agtactgctg ccgcatgaat tccagatgtt acaaagaacc agtccacctg
    11941 aattgcccca gtcaactgta atttcatttg taccctgacc attaactata gtagcacctt
    12001 gtggaactga ccagttataa gttacccctg caagtaaatc aacattataa actattcctg
    12061 tagcatttgc acatactatt gccggaccat tagcaacttt tacagaaggc gcacctttca
    12121 ttgctacgga gcgaatgggt gtttggacac cgcaaactgt tgatgttact tttagttgga
    12181 agtaattaaa accactacta ggatatgtca gtgtaatttg atttccggat tgtaagttta
    12241 ctgttgttcc tgcgggataa ccactccatg aataagttgc atacggatga tttggaatgg
    12301 cagcataagt ttgcgttagt ccgcaaacag ctgaagtctg accactgaag cctgcaattc
    12361 taccgggtga ataatttgta ttgacaaaaa ttacagtaac ctgatctgaa aatacacaac
    12421 caccgtttga cgttgtccat tctaaaacat aaggtttatt gtattgacca ctaaatgtag
    12481 aatttggatt gtttggctga gcaatgctgc caccaacacc ggaaacaata gaccatgaac
    12541 cactgccaac caaaggagca gtagctccta atgttgtact gttaacagaa caaacagcag
    12601 tatcagcacc tgcaatggca gcagaagggc tgttttcatc tacctgtcca ttacaattgt
    12661 catcaattgc attaccacaa atttcagtag caccgggatt gacagcagca ttgttgtcat
    12721 cacaatctgt gctgtctgca acataacctg ccggaggtgt tgggttatta tccaccacaa
    12781 agttgtttag atcaccatag gtgtcggcat cagcatcggc ataccaggtg ttgctgccgc
    12841 aaccttcatc aacctgacca tcgcagtcat catcaatacc gtttccgcaa acttcagtgg
    12901 caccgggata gatggtattg ttattatcgt cacagtcggt gttgttggag acataaccta
    12961 caggagcagt acatgcctgt gtggtcactg cggcatcacc aaaaccgtca ccgtcagtat
    13021 cggcatagaa agtagttgtc acgccctcat caatctgacc attgcagttg tcatcaatac
    13081 cgttacatac ttcagtagca ccgggattga cagcagcatt gttgtcatca caatctgtgc
    13141 tgttggtaac atatcccgga ggtggtgtag ggcttggaga agtaatggag ttgttgagat
    13201 caccaaaagt atcgccatca gtatcggcat aataaacgtt aacggcaaga gaaaagtcat
    13261 caaagcctat gtcgtgtggt gcagtgcgtg tctgacaatc aatatctgta gtaatatagg
    13321 caggtggtgt tgttgctgtt ccatctaatg atgtagaagc agaggtagca tgtaaattat
    13381 ttgcattatt tacaaaatgc ggatcggcaa attgagagtt cacatcagag ttgggattaa
    13441 ttgtcatagc tgcctgccat gctgcctgag tactatacat tgttgtagtt gccttaaaag
    13501 gtgcgccatt ggc
//
LOCUS       DAOQNP010000079        13469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1622_length_13469_cov_8.283957, whole genome shotgun sequence.
ACCESSION   DAOQNP010000079 DAOQNP010000000
VERSION     DAOQNP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13469
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_13469_cov_8.283957"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKY09_10260"
     CDS             complement(<1..476)
                     /locus_tag="PKY09_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13278.1"
                     /translation="MSNLKNKLTLLLAILMLSFLTGNSCSTYKVTVGDKTMYGMNYDT
                     WFSQPRIWFETNGYGAVFTGSNYQGGNDLTPQSGMNEYGLSFGTLATPTPYKVKSFVN
                     KKPIHSRSKYLKDILHSCKNVEEVQAFIEEYDHSTISNDVFFYTDKSGKYLIVEPY"
     gene            complement(974..1855)
                     /locus_tag="PKY09_10265"
     CDS             complement(974..1855)
                     /locus_tag="PKY09_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNB13279.1"
                     /translation="MNLKLLGRDNSIISIILKGLGQIMLQENALCGFLFLAGIFYGSV
                     TMGIAALLAVVCGTATAMLLKYDESEINKGLYGFSAALVGVGVIVFFKPLFICWILIV
                     IGAIAATLLQHFFIKRNVTAFTFPFVLVIWIMLYFISKYLANIQAETALSAINTTDHF
                     AFAFNGYGQVIFQGNFVSGLIFFVAVFISSPIAALYGLAGALLASIVSAYFSVPTENI
                     YLGLFGYNAVLCAIVFAGDHIKDGIWVITSVILSIAISLFMYHNNMIQLTFPFVLASW
                     ITLFIKSKLDFVTLGKN"
     gene            1958..2140
                     /locus_tag="PKY09_10270"
     CDS             1958..2140
                     /locus_tag="PKY09_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13280.1"
                     /translation="MSYKIRFVYYRSIAFLVLWFNRRIQIVIGIEFYTYFDTKSFSPP
                     IAYAILALCHSILSAI"
     gene            complement(2121..2714)
                     /locus_tag="PKY09_10275"
     CDS             complement(2121..2714)
                     /locus_tag="PKY09_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13281.1"
                     /translation="MTLKEFVRQFDKAGSIVLVEGKRNVLEADKKKLTALGKLLTSTT
                     KNILFRSGNAEGSDQLFSDGVTAIDSRRLQVITPYSGHRKKTNQAYATIALDEIDIAA
                     APEVVYQSKSNKKMEKLIDKFVSGDKNRYTIKAAYIIRDTIKAIGTDEIKPATFGIFY
                     DDLDNPMTGGTGHTMNVCKQNSIPIIDQKIWFRWLKE"
     gene            complement(2954..4927)
                     /gene="gyrB"
                     /locus_tag="PKY09_10280"
     CDS             complement(2954..4927)
                     /gene="gyrB"
                     /locus_tag="PKY09_10280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNB13282.1"
                     /translation="MSDQTVENLAEMEVKNTYSADSIQVLEGLEAVRKRPAMYIGDVG
                     VKGLHHLVYEVVDNSIDEALAGHCKNISVTIGEDNSITVKDDGRGIPTEMHSKEKKSA
                     LEVAMTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSTHMRTEVHRNGKIYVQEYSIG
                     KPLYPVKEIGTTDYRGTIQTFKPDETIFITTIYNYDTLASRLRELSYLNKGIRITLTD
                     MREKDEQGNVRSDEFLSELGLREFVEYIDANREKLIPECMFFESDKFGIPIEVAMQYN
                     TSFSENIHSYVNNINTHEGGTHLSGFRRALTRTLKAYAEKEGMLAKLKFDIDGDDFRE
                     GLTAVISVKVAEPQFEGQTKTKLGNNEVMGYVDQAVGDMLVHYLEMHPREAKSIVNKV
                     ILAATARHAARKARELVQRKNVLTGTGLPGKLADCSESDPGRCELFLVEGDSAGGTAK
                     QGRDRNFQAILPLRGKILNVEKAMEYKIYENEEIRNIFTALGIYRDDKQEGRPLILDK
                     LRYHKIVIMTDADVDGSHITTLILTFFFRHMKELIEQGYIYIATPPLYLVKKGKEERY
                     CWTDEERIAIVKELAGEKESSVNIQRYKGLGEMNAEQLWSTTMDPSSRTLRQVTIESA
                     AEADHIFSMLMGDEVPPRREFIERNAKYAKVDA"
     gene            complement(5078..5506)
                     /locus_tag="PKY09_10285"
     CDS             complement(5078..5506)
                     /locus_tag="PKY09_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNB13283.1"
                     /translation="MQKSAIDFRLIKLERNGYHPVCTVTINHKDAIMVIDTGASHTVL
                     DKKQAVRFLPESKIKLSEAKSSGLGTNDMGSAYATFDYFILGDLVLRRLKTIILDLAH
                     INLAYATMQLPLIDGVIGCDILKKYKASINFSTKKLVINI"
     gene            5641..7158
                     /locus_tag="PKY09_10290"
     CDS             5641..7158
                     /locus_tag="PKY09_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNB13284.1"
                     /translation="MAILNSILGWIIKKRMHQIELFMKYPCEVQEELLRKLISTAEHT
                     QWGEHFEYQSIDNFKTFSERVPVVTYDQLQPQVERIMKGEQNILWPSEIKWFAKSSGT
                     TSNRSKFIPVSMESLEECHFKGGKDLLSIYFNNNPDALLFNGKLLSLGGSHSINSFHN
                     ESYYGDLSAILIQNLPFWVQIMRTPEMNIALMDKWDEKIERMAQETIKENVTNISGVP
                     SWTLVLLKRILEITGKKTIAEVWPGLELFVHGAVNFTPYREQFKQIIGKRIQYLETYN
                     ASEGFFGIQDRNDSDEMLLMLDYGIYYEFIPQKYWHEENPKTLTLSEVQTGEQYALVI
                     TTNAGLWRYKIGDTIQFTSISPYRIKISGRTAHFINAFGEELVIDNAEKAITEAGKRT
                     GATVREFTAAPVYFGKKNNGAHEWLIEFSDEPENLDEFTMALDEELKKVNSDYEAKRF
                     KDITLNKPIVHKMPKNTFYKWMEKNKKLGGQHKVPRLANHRKFVEEILSFQNNLS"
     gene            7155..7943
                     /locus_tag="PKY09_10295"
     CDS             7155..7943
                     /locus_tag="PKY09_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13285.1"
                     /translation="MKYFVTLFIIVISCNTFAQQQKTNEIWNGADAFSMDNLGNLYII
                     KESVVFKADTSGRILLTYSNNNQDRFTYVDAIDPLRIMLFADEFSIVSFLDNKLALQS
                     VINLREQNFYDIKAVCNATDGFWCYDHSQQQLFKYNFTLQKIAETQPLALLLNNPLSI
                     TSMCANDKWVACLNNNSGILIFDRLGSYVRSIDAKHASCIMLLENMLVYAENNELIFI
                     DLKLNLETSRLKTDINNISSIKWFHNRFYLLANNKIIQIAYKKD"
     gene            8061..8681
                     /locus_tag="PKY09_10300"
     CDS             8061..8681
                     /locus_tag="PKY09_10300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB13286.1"
                     /translation="MHIAIAGNIGSGKTTLTSLLSKQFKWEPHYEDVNDNPYLNDFYD
                     DMQRWSFNLQIYFLNSRFQQILKIKKHPKTIIQDRTIYEDAYIFAPNLHAMGLMTTRD
                     FQNYQQLFELMNSFVSPPDLMIYLRGTVPALVKQIEKRGRPYENNIRIDYLKRLNERY
                     EAWISTYELGKLLIIDIDEINFAENKEHLGMIIERVQAELSGLFSK"
     regulatory      8728..8922
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9056..10954
                     /locus_tag="PKY09_10305"
     CDS             9056..10954
                     /locus_tag="PKY09_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB13287.1"
                     /translation="MYNYYLLFISFLICTNIQAQTDTFHIKVVNIEALRQQDATPGIK
                     FENIDSISLKQFNTESLSDLLSAESGVIIKNYGPGSLASSTIRGGNANQTAVIWNGFN
                     INNPMLGQTDLSVIPEFFADKISMQYGGGSAQWGSGAVGGAVLLNSATFFNKGVDAEV
                     NIGAGSYGWLRQSFKTNYSNNRWIFNLKAFNDYSSNNFRFQNSITLEKQFQKHARTQG
                     QGVLSEIVYRINKNGTLSIAAWLDKFNRQIPPVLNQTSDAKQKDDNLRITSQWRQQLP
                     KGELAVRAAWFNQDIYYSDSLKNINATSKAKSLLAEAEYKFHLGKHREIHTGINNNYT
                     TATADEYKKEAQLNSFALFIGESAHFFNNKLKPSVSFRKEFIDNKQAPFTWYAGGEIA
                     LFKNISLKLNAASVYRNPTLNDLYWQPGGNKNLEPESGKTGDIGLCLNEINIAKNFYV
                     KTEITLFNRKMKNQIIWLPENGYWSSQNLNRTESKGIETKTNIRYNYQKWFAALNVST
                     NYIEATNEKARFENDASVGKQLIYVPMYSGNGSIAIGFDKLIVKYTIQYSGYRYTTTD
                     NLEYLEPFWLHGFYAACSLTAQKIKFQLYFKINNLFDENYETIRNRPMPLHSYKAGII
                     IQINSKSK"
     gene            10957..11940
                     /locus_tag="PKY09_10310"
     CDS             10957..11940
                     /locus_tag="PKY09_10310"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HNB13288.1"
                     /translation="MKRLRLIIVSMSLFQLVSAKLYAQTVSDFENLTLPADSFFNGSS
                     QQGGTNFISGNATFPNFFDPSFQYWLSGWAYSNVADSSTAGFGNMYACASLTGYNGSG
                     IYAIGQQNATITLSGSAAGKVVNGFYVTNATYPYISMRDGDGFSKKFGGPSGNDPDYF
                     ILTIHNWYNGNLSSDSVNFYLADFRFTNNAQDYIINDWQWVDLTGLGNTDSLLFTLSS
                     SDVGSFGINTPLFYCIDNLTTADSPVAISEIKNDILQVYPNPATSFILLTKKMDDFFI
                     TDVEGRVVMKSFNLNAGDKISVTSLKDGLYFINGQYNNESIKSKFIKCSAH"
     gene            11925..12935
                     /locus_tag="PKY09_10315"
     CDS             11925..12935
                     /locus_tag="PKY09_10315"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13289.1"
                     /translation="MFGSLKTVLIALTCLISLTKSNGQFAPPASQPGTTAIHKDSSII
                     VSWATQCSIVRGWQDISNQSLGICTIGDSTSALGMADGLVVSLGDGGIATLTLTNPIM
                     NGSGWDFAVFENSFSETFLELAFVEVSSDGINFFRFPSVSLTQDTVQVGSFGSIDATQ
                     IDNLAGKYSAFYGTPFDLDIMTGIAGLDVNHITHIRIIDVVGSINEVYATYDSQMHKV
                     NDPWPTPFPSSGFDLDAVGVIHQITTSVNKVKENPFNISYPNPINRNGVIKLNLSNTE
                     YLNITLFDISGKVIYQFSNGEITAGEHHFNLRNSLIDNGIYFLKLSSNEHTETFKLIV
                     ND"
     gene            12928..>13469
                     /locus_tag="PKY09_10320"
     CDS             12928..>13469
                     /locus_tag="PKY09_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13290.1"
                     /translation="MIKQSVYLFLTCILVFSCKKDKPEAVTSTIITSGNSGLVYISNE
                     GNFQFGNAAISLYDKVSKNITEDYYKQVNQVPLGDVCQSMCYFNNKFYIVVNNSSKVV
                     VVNAATFKIEATISGFNSPRYILPVSNSKAYVSDLYDNRLAIVNLSTNSISGYIPAVA
                     GTEQLKMVYGKVFVSSLFTD"
BASE COUNT         4326 a         2491 c         2408 g         4244 t
ORIGIN      
        1 tcatagggtt caacaattag atacttccct gatttgtctg tatagaaaaa tacatcattg
       61 gatattgtac tatgatcata ttcttcaata aatgcttgta cttcttcaac gtttttacac
      121 gaatgtaaaa tgtcttttag gtattttgac ctgctatgaa ttggtttctt gttcacgaat
      181 gattttacct tgtagggtgt gggtgttgct aaagtgccaa acgaaagtcc atactcattc
      241 attcctgatt gtggcgtaag gtcattccca ccctggtaat ttgaacctgt aaaaactgct
      301 ccgtaaccgt ttgtctcaaa ccatatacgt ggttggctaa accatgtgtc ataattcatt
      361 ccatacatgg ttttatctcc cacagttact ttgtaggtgc tgcaagaatt tcctgtaagg
      421 aaagacaaca ttaatattgc cagaagcaaa gtcaatttat ttttcaaatt gctcattagt
      481 cccaatagct taattataat aactccaacg aatttttctt tgcagtatta tacatactgt
      541 tcaaagttag caccgtcctc aaagcttacc gataatatca gtcttaaaaa gacacttcac
      601 aaacgtattt aaaaattaga aaagatttga gctttaaaag tataaatata actactccac
      661 ttgcttataa ttgttggtgt catcagcgaa caaattacat tctcaaatat tctaaaaagt
      721 atctaaaaca ttttagactt cgacaagttg atggaaaccc tcaaccatcg tactctgaac
      781 tatccgtgtg gaatttttca actaaaaaca aggcacaaac aacccaatta aagccatcaa
      841 taatatacat gctgaccagg ttttgttatt tctaatcgct gtacttactt gcttcctgtt
      901 aaattagtgg ataaggaaat tactaatatt atcgccaaca ttattgttga aattaattgt
      961 tcttgttaac atactaattt tttcctaagg ttacaaagtc aagttttgat ttaatgaaaa
     1021 gggttatcca tgaggctaaa acaaatggaa atgtcagttg aatcatgtta ttgtgataca
     1081 tgaacaggct tattgcaata gataaaatca cagaggttat gacccaaata ccatccttaa
     1141 tatggtctcc tgcaaaaact atagcacata atactgcatt ataaccaaat aaacctaaat
     1201 agatattttc agttggaaca gaaaaatatg ctgatacaat ggatgcaagc aatgcgcctg
     1261 caagcccata taatgcagca attggcgaac tgataaaaac tgctacaaaa aatattaaac
     1321 ctgaaacgaa attaccttga aaaataactt gtccatagcc attaaaagca aaggcaaaat
     1381 ggtcggtggt attaatagct gagagagctg tttctgcctg aatattggca aggtacttac
     1441 taataaaata aagcataatc caaatcacta aaacaaaggg aaaagtaaat gcagtaacat
     1501 tacgtttaat aaaaaagtgc tgaagtagtg ttgccgcaat agccccaatg acgattaata
     1561 tccagcaaat aaacaacggc ttaaagaaaa caattactcc aacaccaacc aatgctgcac
     1621 taaatccgta taatcctttg tttatttctg actcatcata ttttagcagc atggctgtag
     1681 cagttccaca aactactgct aaaagtgcag caattcccat cgttactgaa ccgtagaaaa
     1741 tacctgctaa aaaaagaaaa ccacaaagcg cattttcctg aagcattatt tgccctaacc
     1801 cttttagtat gatagatata atggaattgt cccttcccaa tagctttaag ttcatgtttt
     1861 taaagttgct tcctgtaata aatcaatagt tgtttctaat aaatactgat aaatttttct
     1921 acttcaaaca ttcatgtttt taattcttat ttcctcaatg tcatataaga ttcggtttgt
     1981 gtattaccga agtattgcat ttttagtact gtggtttaat cgaagaattc agatagttat
     2041 cgggattgaa ttttacactt actttgatac gaagtcgttc agcccaccta ttgcctatgc
     2101 aatattagcg ttatgtcatt ctattctttc agccatctga accatatctt ttggtcaatt
     2161 atcggaatac tattttgctt gcaaacattc atcgtatgtc ctgttccacc tgtcattggg
     2221 ttgtctaaat cgtcatagaa aataccaaac gttgccggct ttatttcgtc cgttccaatt
     2281 gctttaatag tatctcggat tatataagct gcttttattg tataacgatt tttgtcacct
     2341 gaaacaaatt tatcaataag cttttccatt ttcttattgc tttttgactg atataccact
     2401 tcgggtgcag cagcaatatc tatttcgtcc aaagcaatgg ttgcatatgc ttgattggtt
     2461 ttctttctat gtccggaata tggcgtaata acttgtaatc ttctactgtc gattgcggtg
     2521 acaccatcag aaaacagttg atctgaacct tcggcatttc cacttctaaa taaaatattc
     2581 tttgtcgtag aagtcaacaa ctttccaagt gctgtcaact tttttttgtc tgcttctaaa
     2641 acatttcttt tgccttcaac tagaacaatt gaaccggctt tatcaaactg tcttacaaat
     2701 tcttttaatg tcatattcgt ctgctcttgc attttacact aattataccc ctcatcaacc
     2761 ttcgttaatt aaaccctttt cggtggattg gctaaactac atgcgttttt accctgaaaa
     2821 caaagttatt gttttactta attaaatgaa ttattttaaa tgcgctaaag ttaaagtaca
     2881 aatctttatt cgatattaac gctacaaaaa aagctgccct tatgaggcag cttgcagtaa
     2941 tgtttaaaat ttgttatgca tctactttag catatttggc attgcgctca ataaactctc
     3001 tgcgtggtgg cacttcatcg cccatgagca tgctgaaaat gtggtcggct tcggcagcac
     3061 tttcaatggt aacctgtcgc aaagtgcggc ttgatgggtc catggttgtt gaccataact
     3121 gttctgcatt catttcacca agacctttgt aacgctgtat atttacgctg ctttcttttt
     3181 cgccagccag ttctttgacg atagcaatac gctcttcgtc tgtccagcaa tagcgttctt
     3241 cttttccctt ttttacgaga tagagcggtg gtgttgcaat gtagatgtag ccctgctcta
     3301 tcaattcttt catatgacgg aagaagaatg tgaggatgag tgtagtgatg tggcttccat
     3361 caacatcggc atcggtcatg ataacgattt tgtgatagcg tagtttgtcg agaatgagcg
     3421 ggcggccttc ttgtttatca tcacgataga tgcctagtgc agtgaaaata tttcttatct
     3481 cttcgttttc gtaaatttta tattccattg ctttttctac gttgaggatt tttccgcgta
     3541 gtggaagtat ggcctgaaaa tttctatcgc ggccttgttt ggcagttcca ccggcagagt
     3601 ctccctcgac taagaataat tcgcaacgac ccggatcact ctctgagcag tcagcaagtt
     3661 tacccggcag tcctgttccg gtgagtacgt ttttgcgctg caccagctcg cgtgccttac
     3721 gtgcagcatg acgtgcagtg gcagcaagta taactttgtt gacgatggac tttgcttcgc
     3781 gtggatgcat ttcgaggtag tgaacaagca tatcaccaac agcctgatca acatatccca
     3841 taacttcgtt gttgcccagt ttggtctttg tttgtccttc gaactgtggc tcggcaactt
     3901 tcacagaaat aaccgctgtt agtccttctc tgaaatcatc accatcaata tcgaatttca
     3961 atttggcaag catgccttct ttttcagcat aggcttttaa tgtacgtgta agtgcacgtc
     4021 tgaaacctga aaggtgtgta ccaccttcat gtgtgttgat gttgtttaca taagagtgaa
     4081 tgttttctga aaacgaagtg ttgtattgca tggccacttc aattggaatt ccgaatttat
     4141 cactttcaaa aaacatacat tcaggaatga gcttttcgcg gttggcatca atgtattcaa
     4201 caaattcgcg taatcccaac tcacttaaaa attcgtcaga gcgaacatta ccttgttcat
     4261 ctttttcgcg catatcggtt aatgtaatgc ggattccttt gttaaggtaa gataactcgc
     4321 gaaggcgtga agccagtgtg tcgtagttgt aaattgtggt gataaaaatt gtttcgtcag
     4381 gcttaaaggt ttgaattgtg cctctgtagt ctgttgtgcc aatttccttt acgggataaa
     4441 gcggttttcc gatactgtat tcctgcacat atatttttcc gttgcggtgc acttctgtgc
     4501 gcatgtgtgt actcaatgcg tttacacaag atacaccaac accatgcaat ccaccggaaa
     4561 ctttataaga gtctttatcg aacttaccac cggcatgcag cactgtcatt gctacttcga
     4621 gcgcagactt tttttctttg ctgtgcattt cggtaggaat acccctgcca tcatccttaa
     4681 ctgttattga gttgtcttct ccaattgtga cactgatgtt tttgcaatgt ccggcaaggg
     4741 cttcatcaat tgaattgtca actacctcat aaaccagatg atgcaacccc tttacaccaa
     4801 catcgccaat gtacatggcc gggcgtttcc ttactgcttc taacccctcc aatacctgaa
     4861 tgctgtcggc cgaataagta ttctttactt ccatttcagc taaattttct actgtttgat
     4921 cactcataac ttttcgtaaa ctgtcatttt ttataattgt tcaaatttac aaaaacaaag
     4981 gctactatct cataaaaaca gtgtggaaat acatgttttt tttaacaaag cagcacaagt
     5041 tattaacgca gtttttaagg cttttaatat aaagaaatca gatgttaatt acaagttttt
     5101 tagtagaaaa attgatgctt gccttatact tttttaggat gtcgcaacca ataacaccat
     5161 ctatcaatgg caattgcatt gttgcatagg caaggttgat atgtgccagg tcgagaatga
     5221 tagtttttag tctgcgtaaa accaaatcgc caagtataaa ataatcgaaa gtagcatagg
     5281 cactccccat atcgttggtt ccaagtcctg atgatttagc ttcagaaagt tttattttac
     5341 tttcaggaag aaatcgcacc gcctgctttt tgtctaacac cgtatgtgaa gcaccggtat
     5401 caattaccat tattgcatct ttgtgattga tcgtaacagt gcataccgga tgataaccat
     5461 tgcgctctaa ttttattaac ctaaaatcta ttgctgattt ttgcatgcaa cgaaagtaca
     5521 tcaaacattt cttaaagaaa ttaattactg attaagttca tttaagctga agaattaatt
     5581 ttgcagaatg gaagttgcaa ggattttacc tatctttccg ttcgcaaagc actgtatagt
     5641 atggcaatac tgaattcgat attggggtgg ataattaaaa agcgcatgca tcagattgag
     5701 ctttttatga aatatccatg cgaggttcag gaagagttgc tcagaaaact tatttcaaca
     5761 gcagagcata cacaatgggg cgagcatttc gagtatcaat ccattgacaa ctttaagact
     5821 tttagtgagc gtgtaccagt agttacttac gatcaattac agccacaagt tgaaagaata
     5881 atgaaaggtg aacaaaatat tttatggcca tcggaaataa aatggtttgc caaatcatct
     5941 ggaacgactt caaacaggag taagtttatt cccgttagta tggagtcatt ggaagaatgt
     6001 catttcaaag gcggcaaaga cttgctttca atttatttta acaacaaccc cgatgccctc
     6061 ctgttcaacg gcaaactgct ttcgcttggt ggcagccaca gcataaattc atttcataac
     6121 gaatcttatt acggtgatct ttctgccatt ctgattcaaa accttccatt ctgggtgcag
     6181 ataatgcgaa ctccggaaat gaatattgca ctaatggata aatgggatga aaaaattgag
     6241 cgcatggcac aggaaacaat taaggagaat gtgactaaca tttctggtgt gcccagttgg
     6301 acattggtgt tgttaaaaag aattcttgaa ataacaggaa aaaaaaccat tgcagaggtg
     6361 tggccgggct tagaattatt tgttcatggt gctgtaaatt ttacgccata cagagagcag
     6421 tttaaacaaa ttatcggaaa acgcattcag tatttagaaa cctacaatgc ctccgaagga
     6481 ttttttggta ttcaggacag aaatgattct gatgaaatgc tgctgatgct cgattatgga
     6541 atctattatg agtttattcc tcaaaaatat tggcatgagg aaaatccaaa aacgctgaca
     6601 ctttctgaag ttcaaacagg agagcaatat gctttagtca tcacaaccaa tgctggtcta
     6661 tggcgttata aaataggcga taccattcag tttacttcca tcagtcctta cagaataaaa
     6721 atcagtggac gtactgcaca ttttataaat gcatttggag aagaattagt gattgacaat
     6781 gcagaaaaag caattactga agcgggtaaa agaacaggag caacagtgcg cgaatttact
     6841 gctgcaccgg tttatttcgg caagaaaaat aatggcgctc atgagtggct gattgaattt
     6901 tcagatgagc ctgaaaactt agatgagttt actatggcac ttgatgaaga gcttaaaaaa
     6961 gtaaattccg actatgaggc aaaacgattc aaagacatca cactcaacaa acctattgta
     7021 cataaaatgc ctaagaacac tttctataaa tggatggaaa aaaataaaaa attaggaggg
     7081 caacataaag ttccaagact tgccaaccat cgaaaatttg ttgaagaaat attaagcttt
     7141 caaaataatc tgtcgtgaaa tattttgtaa cattattcat catagtcatt agctgcaaca
     7201 cctttgcaca gcaacaaaaa acaaatgaaa tctggaatgg tgctgatgct ttcagtatgg
     7261 ataaccttgg caacctctat attataaaag aaagtgtagt ttttaaggct gatactagtg
     7321 gaagaatttt gcttacttac agcaataaca atcaggatag gtttacctat gttgatgcca
     7381 ttgacccgct gcggattatg ctttttgctg atgaattttc tattgtcagt tttttggata
     7441 ataaattagc cttgcaatca gtaatcaatt taagagaaca aaatttctat gacataaaag
     7501 ctgtttgcaa tgctacagat ggattttggt gttatgacca ctcacaacaa caactcttta
     7561 aatataattt tacacttcag aaaattgctg aaacacaacc gttggcatta ttattaaaca
     7621 atccactatc tataacgagt atgtgtgcca acgataaatg ggtggcatgc ctgaacaaca
     7681 actcagggat tttaattttt gacagactgg gaagttatgt aagaagtatt gatgcaaaac
     7741 atgcatcatg tataatgctt ctcgaaaaca tgttggttta tgctgaaaat aacgaactga
     7801 tttttattga cctgaagctt aatttagaaa cgtccagact gaaaactgat atcaataaca
     7861 ttagtagcat caaatggttt cataaccgtt tttacctgct tgccaataat aaaataatac
     7921 agatagcgta taaaaaagat tagaactgtc aataataaag gctattacgt aagtaactgc
     7981 atttgccaat gaattttttt ttactttagc gcacaacttt ttgagaacta tattatttaa
     8041 aaccagattg atttaattgt atgcatattg caatagcggg aaatattgga tccggaaaaa
     8101 ctacactcac cagtttatta tcaaaacagt ttaaatggga accccattac gaagacgtga
     8161 atgacaatcc ctatctaaac gatttttatg atgacatgca acgctggtcg ttcaaccttc
     8221 agatttattt tctgaatagc cgctttcagc agattttaaa aatcaagaag catccaaaaa
     8281 caattattca ggacagaacc atttacgaag acgcatacat ttttgcacct aacctgcatg
     8341 ctatggggtt aatgacaaca cgcgattttc agaactatca gcagttgttt gagctgatga
     8401 acagttttgt ttctccacct gatttgatga tttatctgcg tggtactgtt ccggctctgg
     8461 taaagcaaat tgaaaaacgt ggaaggcctt acgaaaataa tatacgtatt gattatctga
     8521 aacgacttaa cgagcgttat gaagcctgga tttcaactta tgaacttggc aagctgctga
     8581 ttattgatat tgatgaaatt aactttgctg aaaacaaaga acatctggga atgattatcg
     8641 agcgtgtgca agccgaactc agcggtttat tcagcaaata agcatcttct atttttttat
     8701 ttatatttgc cgcagaaagt tctttcattc atttcaaccg gtaaaggttt cttctgttta
     8761 aaaactgaag atataaaagg gaatcacgtt aaaatcgtga gctgtagcgc aactgtacgt
     8821 aacgcatcaa catatttctg ctgtatttac tgcaatattt gtaggaagta aaagagataa
     8881 aagttacaag ccaggatatc tgtctttact gaggctgttc attcagccat tgaccatgct
     8941 ttcgcattaa aagcagaagt ctgtattgat acaaccataa acaaaggttg tattattcct
     9001 cacttttttt tactgcactg catgtagaaa tgcttgacaa aaaccaagca aacacatgta
     9061 taactattat ctgttgttta tttcttttct catttgcacc aatattcagg cgcagacaga
     9121 tacttttcac atcaaggtag ttaatattga agcattgcga caacaagatg ctacaccggg
     9181 aataaaattt gaaaatattg acagcatcag cctgaagcaa ttcaatactg aatcgttaag
     9241 tgaccttctt tcagccgaaa gtggtgttat tattaaaaat tacggaccgg gttcattagc
     9301 ttcttcaact atcagaggtg gtaatgcaaa tcagactgca gtaatctgga atggtttcaa
     9361 catcaataat cctatgctgg ggcagacaga cctttctgtt atccctgaat tttttgctga
     9421 taaaataagt atgcaatatg gtggtggcag tgcacaatgg ggaagtggtg ctgttggtgg
     9481 tgccgtttta ctcaattcag ctacattttt taataaagga gttgatgcag aagtaaatat
     9541 tggtgcagga agctatgggt ggctgcgaca atcatttaaa acaaactata gcaacaacag
     9601 atggatcttt aatcttaaag catttaacga ctattcgtca aataattttc gttttcaaaa
     9661 ttctatcacg cttgaaaagc aatttcaaaa acatgcacgc actcaaggac aaggtgtgtt
     9721 aagcgaaatt gtttaccgaa taaataaaaa tggcacacta agtatagctg catggcttga
     9781 taaattcaac agacaaattc ctccggtact caatcaaaca tctgatgcca aacagaaaga
     9841 tgacaatcta agaatcactt cgcaatggcg tcaacagttg cctaaaggag agttagctgt
     9901 tcgtgctgca tggttcaatc aggacatata ttattcagat agcttaaaga acataaatgc
     9961 aacaagcaag gcaaagtcat tgctggcaga agctgaatat aaatttcatc tgggcaaaca
    10021 cagagaaata catacaggaa taaacaacaa ttacaccaca gcaacagcag acgaatataa
    10081 aaaggaagca caattaaact cttttgcact tttcattggt gaatcagcac atttttttaa
    10141 caataaatta aaaccatcag tatcattccg aaaagaattt attgataaca agcaagcacc
    10201 atttacctgg tatgcaggag gagaaattgc attgtttaaa aacatttctt tgaaattaaa
    10261 tgctgcaagt gtctatcgca atccaaccct gaatgacttg tactggcagc cgggtggaaa
    10321 taaaaatctg gaacctgaat caggaaaaac aggtgacatc ggtctttgtc ttaatgaaat
    10381 caacattgca aaaaacttct atgtaaagac tgagattaca ctctttaaca gaaagatgaa
    10441 gaatcagatt atatggctgc cggaaaatgg ttattggtct tcacaaaatc ttaacagaac
    10501 agaaagcaag ggcatagaaa caaaaacgaa catacgatac aactatcaaa aatggtttgc
    10561 tgctttaaat gtttcaacaa attatattga agccaccaat gaaaaagccc gttttgaaaa
    10621 tgatgcttct gtgggcaaac aattaattta tgtacccatg tactccggca atggaagtat
    10681 agcaattgga tttgacaaac tgattgtaaa atatactatt cagtattcag gctatcgtta
    10741 cactacaaca gataatcttg aatatttaga accattttgg cttcatggat tttatgccgc
    10801 atgcagcctg acagcacaaa aaataaaatt tcaactttac tttaaaatca acaacctgtt
    10861 tgatgaaaac tatgagacaa tacgtaatcg tccaatgccc ttacattcat acaaagcagg
    10921 aatcattatt cagataaatt caaaatcaaa ataaatatga aaagactcag actcatcatt
    10981 gtatccatgt cactatttca attagtgtct gcaaaacttt atgcacaaac ggttagcgat
    11041 ttcgaaaacc tgactttgcc ggcagatagt tttttcaacg gatcgtcaca gcaaggagga
    11101 actaatttca tttcaggaaa tgctactttt cctaacttct tcgacccttc atttcagtat
    11161 tggctttctg ggtgggcata cagcaatgtt gctgatagca gcactgcagg ctttggaaat
    11221 atgtatgcct gcgcttcact gacaggatat aatggctcag gcatttacgc aataggtcag
    11281 caaaatgcaa ccataacact ttcaggtagt gctgctggca aagttgttaa cggcttttat
    11341 gttaccaatg caacctatcc atacatcagc atgagagatg gagatggttt ttcaaaaaaa
    11401 ttcggaggac catcaggaaa tgatccagat tattttattc tgaccataca caattggtat
    11461 aatggaaatt taagcagtga tagtgttaac ttttatcttg ctgattttcg ttttacaaat
    11521 aatgctcagg attatatcat caacgactgg caatgggtag atttaacagg acttggtaac
    11581 actgacagtt tgctttttac gctttcctca agtgatgttg gaagttttgg catcaacaca
    11641 cctttgtttt actgtattga taacctcact actgccgatt cgcctgtagc aatctcagag
    11701 attaaaaacg acatattaca agtttatcca aatcctgcaa cttcctttat cttattaacc
    11761 aaaaaaatgg atgatttctt tatcactgat gttgaaggtc gggttgttat gaaatcattt
    11821 aatttaaatg caggagataa aatatctgta acatcactca aagacggatt atattttatc
    11881 aatggacaat ataacaacga atcaataaaa tctaaattta taaaatgttc ggctcattaa
    11941 aaacagtatt gattgcattg acttgcttga tttcattaac taaaagcaat ggtcagtttg
    12001 caccacctgc cagtcagccg ggcactacgg caatacataa agacagcagc attatagttt
    12061 cttgggcaac tcaatgcagc attgtcagag gatggcagga catttctaat cagagtttag
    12121 gtatatgtac aattggcgac agcacttctg ctttgggaat ggctgatggc cttgtagtta
    12181 gccttggcga tggtggcatt gccacattaa cattaacaaa tccaattatg aatggaagtg
    12241 gatgggactt tgcagtattt gaaaatagtt ttagcgaaac atttcttgaa cttgcatttg
    12301 ttgaagtgag tagcgatggc ataaactttt ttcgttttcc atcagtgtcg ttaacacaag
    12361 atacagtgca ggttggaagc tttggaagca ttgatgcaac acaaattgac aacctggcag
    12421 gcaagtacag tgcgttttac ggtacgccat tcgacttaga tatcatgaca ggaattgcag
    12481 gccttgacgt aaatcatatc acccatatca gaataattga tgtggtagga agtattaacg
    12541 aagtttatgc tacatacgat tctcaaatgc ataaggttaa cgatccttgg ccaacaccgt
    12601 ttcccagttc cggcttcgac cttgatgcag taggtgtcat tcatcaaatt acaacttctg
    12661 taaataaagt taaagaaaat ccatttaata tctcctaccc caatcctatc aacagaaacg
    12721 gtgtgataaa attaaatctt tcaaatactg aatatctgaa cataacattg tttgacattt
    12781 caggaaaagt tatctatcag ttttcgaatg gagaaatcac tgcgggtgaa catcatttca
    12841 atcttagaaa ttctttaatt gataatggaa tttacttttt aaaattatca tcaaatgagc
    12901 atacagaaac ctttaaactt attgttaatg attaaacaat ctgtttattt atttcttact
    12961 tgtattcttg ttttttcctg taaaaaagac aagcctgaag cagtaacatc tactataatt
    13021 acctctggca acagtggctt ggtgtatatt agcaatgaag gaaattttca gtttggaaat
    13081 gccgcaatca gtctgtatga taaagtatcc aaaaatatta cagaagatta ttacaagcag
    13141 gtaaatcaag ttcctttagg tgatgtttgt cagagcatgt gttactttaa caataagttt
    13201 tatattgtag tcaacaattc ttctaaagtt gtggtagtta atgctgctac atttaaaatt
    13261 gaagcaacta tttcaggttt taattcacca cgctatattc ttcctgttag caatagcaag
    13321 gcatatgtat cggatctata cgacaacaga ttggcaattg taaatctttc aacaaacagt
    13381 atctcaggct atataccagc cgttgcaggt acagaacaat tgaaaatggt ttacggaaaa
    13441 gtctttgttt catcactctt caccgataa
//
LOCUS       DAOQNP010000080        13331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1641_length_13331_cov_7.815908, whole genome shotgun sequence.
ACCESSION   DAOQNP010000080 DAOQNP010000000
VERSION     DAOQNP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 13331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13331
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1641_length_13331_cov_7.815908"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..529)
                     /locus_tag="PKY09_10325"
     CDS             complement(155..529)
                     /locus_tag="PKY09_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNB13291.1"
                     /translation="MHLSLKFKILITLFTLFSFYTAFVYTKATARATFTMNDEAFKGK
                     MLYQKFNCTACHQLYGLGGYLGPELTSAYTQKAQNDAYFSAILKNGTVRMPDFNLSDK
                     EVEQVIEYLKYIDQTSKQVPAY"
     gene            complement(715..1230)
                     /gene="paaJ"
                     /locus_tag="PKY09_10330"
     CDS             complement(715..1230)
                     /gene="paaJ"
                     /locus_tag="PKY09_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HNB13292.1"
                     /translation="MVKDSMKEQSSLESSEIFQLLYAIPDPEVPVINIGELGVLRSVD
                     WINGQLIITITPTYSGCPAMFAIESEIKTILKDNNISNFKVKTVYFPAWTTDWLTDEA
                     KRKLSDYGIAPPEHSSHIKQLRPDEVSVRCAFCHSEETHLTSWFGSTACKALYYCDNC
                     KQPFEHFKCHH"
     gene            complement(1224..1979)
                     /gene="paaC"
                     /locus_tag="PKY09_10335"
     CDS             complement(1224..1979)
                     /gene="paaC"
                     /locus_tag="PKY09_10335"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HNB13293.1"
                     /translation="MNKDLFEYTLRLGDTSLILSQRLGEWCGHGPMLEEDIALTNIAL
                     DLLGQAKAFLAYAGEIEGKGRNEDVLAFHRDANHFRNLLLTEQPNVDFGFTIIRQLFV
                     STWQHYLYSELTHATDKNMSALAVKSLKEVNYHLRFASDWTLRLGDGTEESHQRVQAA
                     VNELWNYTGDLFEIDQVDEALLKLGIAADMKKVEASWNKHIRQVLNDATLSVPEINNF
                     MRTGSRKGVHTEYLGFILAEMQFLPRAYPDAKW"
     gene            complement(1985..2272)
                     /gene="paaB"
                     /locus_tag="PKY09_10340"
     CDS             complement(1985..2272)
                     /gene="paaB"
                     /locus_tag="PKY09_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HNB13294.1"
                     /translation="MSDKQLQLWEVFTQKKTGMSFEHAGSVRAADKEMALLNARDVYS
                     RRGEATCIWVVPSECISSSSPEDIASFFEPSDDKIYRHPNFYKTPDGIKQV"
     gene            complement(2314..3258)
                     /gene="paaA"
                     /locus_tag="PKY09_10345"
     CDS             complement(2314..3258)
                     /gene="paaA"
                     /locus_tag="PKY09_10345"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HNB13295.1"
                     /translation="MEVNIEQLEKDFQAKIDAGLSIEPKDWMPEKYRHQLIRMMSQHA
                     HSEVVGMLPEGNWITRAPSLMRKMVLLAKVQDEAGHGLYIYSATETLGISRDEMINQL
                     LTGKAKYSSIFNYPTLNWADMGAIGWLVDGAAIVNQTALAKCSFGPYARAMLRICKEE
                     NFHQKQGYQILANMMQGTEAQKQMVQDAVNRFWWPSLMMFGPSDKNSPNSDELMRWKV
                     KLYSNDDLRQRFIDRTVPQAEAIGIKIPDDKLKWNEKTRHYEWGEINWDEFYAVIGGN
                     GPCNRLRMKQRNDAHKNGQWVREAAIAFAEKRKKNKAA"
     gene            complement(3297..4073)
                     /locus_tag="PKY09_10350"
     CDS             complement(3297..4073)
                     /locus_tag="PKY09_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNB13296.1"
                     /translation="MSYTNLNYNVENGLATITINRPDKLNALNALTVQEIGAAVKKAI
                     NQSEVRIIIITGSGEKSFVAGADISEFEGLNEEQSRALAQAGQDAFFVIENSPKPVIA
                     AINGFALGGGCELAMACHLRVAAENARFGQPEVKLGLIPAYGGTQRLTKLIGKTKALE
                     LMITTDMINAAEALQMGLVNYVVPQAELMNKTRELASKIMKQAPLAVAGVIRCVNDFY
                     KQVNGFQTEVEVFGQCFKTEDNKEGVAAFKEKRSPVFKGK"
     gene            4209..4994
                     /locus_tag="PKY09_10355"
     CDS             4209..4994
                     /locus_tag="PKY09_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNB13297.1"
                     /translation="MSFISKKQAIPLLSILFLATSCVPSKKFLYYQNGLEQKSHIDST
                     ISLKAYVHRISPNDVLTIFVSSLSPEASAYFNPPINQDNLPDVATYKALSSYTVDSNG
                     EISLPFVGLLKIKGLTIPEVRDTIEKSLEKYLINPTVFINLENFKIVLLGEVTKPGVF
                     FVNNEHITISEALAMAGDMTVFGKRTNVLIVRETMQGNPEYGMVDMTSPDVFKSPYYY
                     LHPNDIVYVNPRKNKIASGDLFFRIAPILISLATLVSLLVIRL"
     gene            5077..7446
                     /locus_tag="PKY09_10360"
     CDS             5077..7446
                     /locus_tag="PKY09_10360"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNB13298.1"
                     /translation="MKQFIYEKIFYYWYIYILCMILALVTANYYLWYVSPVYTSTTSV
                     MLDIDEKKLASQDLLASLGSIENTGGIESQMQIIKSRNIITRTIQQLDFRFSYFLEGD
                     IKTSELYKNCPFEVITDSLTITNNIPDLTIRVTSVSTFDISYINPKTNSSHTNSLVFN
                     TPFTTDFGIFTLKLKEPKILKIYKNSINEKNEYIIKAKTTADLVQYYSSRLNVTQIKG
                     TRVLMISVDDAVPQKGCDFLNKLTDVYLDFGIEQKNEDTENSLRFIDGQLKIITNELL
                     NSEGKLEKYKTEKGLIDLPGMLTIAQEDVSRIENEISTIEIKQSVLKYLNDYIRNGNN
                     VKYLAPAYVEVTEPILQNLIANLSALQSDLIQSLKVTTVDNPIIQKKTIQIENVKAEI
                     LEKIKSISDASIITKNEIYHQLNKLKSKLSEMPKAERELSNIARQANITEQLYIYLLQ
                     KRAETAIILASTISDSKVIDNARTILPPVKPDKGITYTISILLGLFIPASIVYFKEIL
                     DDRIKSRKMLEKSSTIPVLGIIGLTVTENNIVVNLKPHSQVAEAFRSIRTNLQYFHGA
                     KTNNIILITSSISAEGKSFCAVNLAAILASGGKKVVMIGCDLRKPQQPANFNLTSSTG
                     LSNYLIDSATVEEIIQHAPDLGLDLILSGPKPPNPSEILLNKRMGDLIELLKAKYDYV
                     IIDSPPIGLISDGLELSRFADVNIYIVRQNFTRKLHLDFINKIYMDGKMKNLCILFNG
                     VQLGSTIYGYGYGYGYSYGYGYGYGYYEEDKKPKNFFQRLIYKFKFKNN"
     gene            7477..9282
                     /locus_tag="PKY09_10365"
     CDS             7477..9282
                     /locus_tag="PKY09_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB13299.1"
                     /translation="MKRKITIRNDFFSYFKYFFRILKDKFLISIFLSIGVSFLDGIGM
                     AMFMPLIQSVSDDGQSVSFSSAALGKLHYFTDGLENMGINLNLYSILVVLIILFTLKG
                     VFRFFQQYYQVKMCQFYMKTVQVDLTNHILNLSYEGFLTFDAGKIQNSLTTEVNRMRN
                     GIMHYLTSFQSIIMLLTYMLLAYLANWKFALLVSLGAVISNVVFKRVFRLTKSASNSL
                     SKKGNQFNSYILQAVHYFKYLKSTNYFSHYSKKLKTTIEETEFLNKKIGLYQSITSSV
                     REPVLIIVVSLVILMQVTFFGSGLSTIMLSLLLFYRALIYLLSLQASWQSFIQNIGAF
                     ESVNSTLSHMKTLEEVNTGTKTFSFNHKIQLRNVTLQYGKKVILNDITLNIDKNKTIA
                     LIGESGSGKTTLANLIATLLKPTKGDILFDNNPATLYDLKTYRNKIGYISQEPVIFND
                     TIYNNIAFWQEISPENMANFWKVAKLTHLEDFIKSLKDKENTRLGDNGILISGGQKQR
                     ISIARELYKNVELLILDEATSSLDSETESIIQKNIDTLHGNFTIVIIAHRLSTIKNAD
                     VIYLIEKGKIVDSGSFNELIEHSTKFKRMVELQEF"
     gene            9635..9772
                     /locus_tag="PKY09_10370"
     CDS             9635..9772
                     /locus_tag="PKY09_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13300.1"
                     /translation="MTNTKAKSTHVGKEYRGTKKIGTQIIATDAGKNKSSDEKQKMLA
                     I"
     gene            9842..10792
                     /locus_tag="PKY09_10375"
     CDS             9842..10792
                     /locus_tag="PKY09_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNB13301.1"
                     /translation="MNITIAGGGAFGTALANSLKKEHKITIHTRNKQVIHEINCYRKN
                     ISYFPNKVLSKQIKASDDLTVANQADFLFLCIPSHAIVSYIKLLNLNSNCIIINGSKG
                     FGDKDQLIPEIISHLMKNKIASLKGPSFANELIFEIPTSFTIATSEPDTFEEIKSLFR
                     TEVAIFDYSSDIKGVEVLSILKNIYAIIVGIVDAYYNSPNVRFLVFAKALNEIKAILE
                     FLNIPNETLFRYAGIGDFGLTSLNDLSRNRTLGLLIGKGFFGKNPGNPVVLEGVKAIE
                     HFAGKIDTEQQYDLKYIRLLQKLLNNETNVKDFVNKVIYT"
     gene            10826..11974
                     /locus_tag="PKY09_10380"
     CDS             10826..11974
                     /locus_tag="PKY09_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB13302.1"
                     /translation="MHKRIINKLICPNSQSKQLQVHAIEALRNNNIIHNIYDENILPD
                     DDIKTGFIFSTDKKYIYFIYEYIPILLNDQDQDSAHLNQIYEWIKNSIPDEFKDAVTA
                     NILRIRKANESTDGNWNREEMKYYDAAVDTPELRDKMLNSIQRENLWRIFIPRKEEMI
                     NHFEKNCKGQWIFEIGCGNARTVARMFNPVKFNYNYIGTDISFKRLMVAKMAIPESDF
                     IQASALNIPIKNNSFNGAISFGMLHHLPRPAEAVTEVNKKLLQGAIFSYHEPVYTSRQ
                     FSEKQSAIVRKVFRTYDHSDHDNKINLKESLAALKEANHQIVSIVPYNSVFRVVFEAI
                     INRFYKSWQKSKLVMKSVLIIDNILLKTIVRLSNKLGPQAIIVVSKKL"
     gene            12211..13008
                     /locus_tag="PKY09_10385"
     CDS             12211..13008
                     /locus_tag="PKY09_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNB13303.1"
                     /translation="MKNYLDKKINKYVTGVFLHGSLGTNELVQYSDFDALIILNQSTF
                     DSKKSIINVATTLKKSERIMQKMDPLQHHGWFVLTEKELDDFPTSYFPPSLFEQAKCL
                     MGKNKFQIHFQNPSNVTKLQLFKSFCNHLLSNINRIEQNLNYYEVKSLLSGFMLLPSV
                     YLQLNSLEGVFKKESFNLLKGTLCQEEYEIMNQVSEIRANWDYQPPILYKVILSLNSF
                     ALNHFLKKKTSGKIPTKIKNKLLDMRPAMTNFIQTLLTKAKHEVQID"
     gene            12989..>13331
                     /locus_tag="PKY09_10390"
     CDS             12989..>13331
                     /locus_tag="PKY09_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13304.1"
                     /translation="MKYKLIDKPHFVSHEIYNEVTDKLISKLASNANVIAIYTIGHVG
                     NPGISDIDMLIIVKDNCKLTHNFRADMSDIEKYLFLHQPYACSESGFKKAETFSFFHN
                     YNLVHGIDVRTS"
BASE COUNT         4593 a         2374 c         2094 g         4270 t
ORIGIN      
        1 ataaattaag gttgagtttg ttttcatcgt catctttttt cttacaagat gaaaccatta
       61 aggtcatcag tactaacaat acgacatttc gaattaataa gattgaagtt ctcatacttt
      121 attgtattta ttgtttgttt tcatgtttag acttctagta agcaggtact tgttttgatg
      181 tttgatcaat atatttcaaa tactcgatta cttgttctac ctctttatca ctcagattaa
      241 aatcgggcat acgtacggtt ccgtttttta gtattgctga aaagtatgca tcattttgtg
      301 ctttttgcgt ataggcagat gtcagttcag ggccaagata tccacctaaa ccataaagtt
      361 gatgacaggc agtgcagtta aatttctgat aaagcatttt ccctttaaat gcttcatcgt
      421 tcatagtaaa ggtagcacgt gctgttgcct tggtgtaaac aaacgcagta taaaaactaa
      481 ataatgtaaa aagtgtaatt aatatcttaa actttaaact caaatgcata actgaatcgt
      541 ttttacattt ctctttttac agaaatgttt gaatgatcat acacaaatga acaattatcc
      601 ttaagaaatc atattgatta atgacagcga taaggctgac ttttatcact gatcaaaata
      661 gtattagaat agattgataa ataaatattt agataaatct gcttaatgaa aaaatcaatg
      721 atggcactta aaatgctcga atggttgttt acaattatca caataataaa gtgctttgca
      781 tgctgttgag ccaaaccaac tggtaagatg tgtttcttcc gaatgacaaa aggcacatct
      841 tacacttacc tcatcaggtc gcaattgttt tatatgtgac gaatgttcag ggggtgcaat
      901 accataatcc gaaagttttc tttttgcttc atcagtaagc cagtctgtag tccacgcagg
      961 aaaatacact gtcttaactt taaaatttga gatattatta tcttttaaaa tggtcttaat
     1021 ttccgattca atagcaaaca tagcaggaca tccggagtat gttggtgtaa tggtaataat
     1081 gagttgtccg ttaatccaat caacacttct caaaacacct aactcaccaa tattaattac
     1141 cggcacttca gggtcaggaa tggcatacag taactgaaaa atttctgatg attctaaact
     1201 actttgttct ttcatggaat cctttaccat tttgcatcag gatatgcacg aggcaaaaat
     1261 tgcatttcag caagaataaa tccaagatac tctgtatgaa ctcctttacg gcttcctgtt
     1321 cgcataaaat tattaatctc cggcacagat aacgtggcat cattcagcac ttgcctgata
     1381 tgtttattcc aacttgcttc tacctttttc atgtcagcag caattccaag ttttaacaac
     1441 gcctcatcaa cttgatcaat ctcaaaaaga tctccggtat aattccatag ttcattgact
     1501 gcagcctgca ctctttgatg actttcttca gtcccatctc ccaaacgcaa tgtccaatcc
     1561 gaagcaaaac gcaaatggta gttaacctcc ttcaatgatt taacagctaa ggcactcata
     1621 tttttatcag tagcgtgtgt gagctcagaa tataaataat gctgccacgt acttacaaaa
     1681 agttgtctga taattgtaaa accaaaatca acattaggtt gttcagtcag caataaattt
     1741 ctgaaatggt ttgcatctct atgaaaagcc aaaacatctt cattacgacc tttaccctct
     1801 atttcaccgg cataagcaag aaacgctttt gcttgtccta acaaatcgag tgcaatattg
     1861 gtgagcgcaa tatcttcttc aagcattgga ccatgcccac accattcacc caaacgctga
     1921 cttaaaatga gacttgtatc acctaaacgc aatgtgtatt caaataaatc tttattcatg
     1981 attactaaac ctgcttaatg ccatcaggcg ttttataaaa gttcgggtga cgataaattt
     2041 tatcatctga aggttcaaaa aaggaagcaa tatcttcggg agaagaagat gaaatacatt
     2101 cagaaggtac tacccaaatg caggttgctt cgccacgtct gctgtaaaca tcgcgtgcat
     2161 ttagtaatgc catttcttta tctgctgctc ttacacttcc tgcatgctca aaagacattc
     2221 cggttttctt ttgggtgaac acctcccata attgcaattg tttgtcggac attgtgaact
     2281 aaaattaaaa taattattgt tgaaattata gacttatgcc gctttattct ttttgcgttt
     2341 ttcagcaaat gcaatagcgg cttcgcgcac ccactgacca tttttatgtg catcgttgcg
     2401 ctgcttcatt ctaagtctgt tgcaggggcc attaccacca ataactgcat aaaactcatc
     2461 ccagttgatt tcaccccatt catagtgacg tgttttttca ttccacttca acttatcatc
     2521 cggaattttt atgccgattg cttcagcctg tggcactgtt ctgtcaataa accgctgacg
     2581 caaatcgtca ttgctgtaaa gtttaacttt ccaccgcatg agttcatcgg agttcggaga
     2641 gtttttatca ctcggaccaa acatcatcag tgatggccac caaaacctgt tcactgcatc
     2701 ctgaaccatt tgcttttgcg cttcagttcc ctgcatcata ttggctaaaa tctgatagcc
     2761 ttgtttctga tgaaaatttt cttctttaca gatacgtagc atagctcttg catagggacc
     2821 aaaagaacat tttgccaatg cagtctggtt tacaattgct gcaccatcca caagccaacc
     2881 tattgctccc atgtctgccc agtttaatgt ggggtaatta aaaatacttg aatattttgc
     2941 tttacccgtc agtaactgat taatcatttc atcacgactg atgcctaaag tttctgtggc
     3001 actgtagatg tacaatccat gccccgcttc gtcctgtact ttggctaaca acaccatctt
     3061 tcgcattaac gatggtgctc gtgtaatcca gttaccttca ggcaacattc caaccacctc
     3121 actgtgagca tgctgcgaca tcattcttat gagttgatgt ctatactttt caggcatcca
     3181 atctttaggt tctatactta aacctgcatc aatctttgcc tggaaatctt tttccaattg
     3241 ttctatattt acctccataa aattttaata aaaaatcaat attagtaact acatagctac
     3301 ttacctttaa aaacaggact tcttttttct ttaaaagctg caacaccttc tttgttatcc
     3361 tctgttttaa aacactgtcc aaaaacttct acttccgtct gaaagccgtt tacctgtttg
     3421 taaaaatcat tcacacaacg aatgacacct gcaacagcca atggtgcctg cttcataatt
     3481 tttgaagcca attcacgcgt tttattcatc agttctgctt gtggaaccac ataatttacc
     3541 aagcccatct gaagtgcttc ggcagcattt atcatgtcgg tagtaatcat caattccaat
     3601 gcttttgtct taccaataag tttggtcagc cgctgtgttc caccataagc aggaatcagg
     3661 cctaatttaa cctcaggctg tccgaatctg gcattttctg ctgcaacacg cagatggcat
     3721 gccatagcca gttcgcagcc accaccaaga gcaaaaccat tgatggcagc aataacaggc
     3781 tttggcgaat tttcaataac aaaaaaggca tcttgtccgg cttgtgccaa tgcccttgat
     3841 tgttcttcat ttaatccttc gaactcagaa atatctgcac cggcaacaaa tgatttctct
     3901 ccagatccgg taataataat tatgcgtact tcgctctgat taatagcctt ttttactgca
     3961 gcaccaattt cctgaacagt taatgcattt agcgcattta gtttatcagg acggttgatg
     4021 gtaatagtag caaggccatt ttcgacatta taatttaagt ttgtataaga cataaaagta
     4081 aaaaatgtat tagctgcgaa attatgaaaa tgtaagctac aaacaatata taaatgtata
     4141 gcagaagctt aattagctta atttttctat tatctttgcc gcccattaca tttgaagaaa
     4201 atatttcaat gagttttata tcgaaaaaac aagccatacc gctgctttct attctatttc
     4261 tggctacatc ttgtgtacct tctaaaaaat tcctgtatta tcaaaatggg ttggagcaaa
     4321 agtcacatat agacagcact atatctttga aggcttatgt ccatagaata tctccaaacg
     4381 atgttctgac catttttgtt tccagcctaa gtccagaggc aagtgcctac tttaatccac
     4441 ctatcaacca agacaatttg cctgacgtag ctacttataa agcattaagt agctatacag
     4501 ttgatagcaa tggcgaaatc agcttaccat ttgttggtct tttaaaaatt aaaggactta
     4561 ccataccaga agttagagac actatagaaa aatccttaga aaagtatctt atcaacccaa
     4621 ctgtattcat caatcttgaa aattttaaaa ttgtactact cggtgaggtc actaagcctg
     4681 gtgttttttt tgttaataat gagcatatca ccatttctga ggctttagca atggcaggag
     4741 acatgacagt ttttggtaaa agaaccaatg tgcttattgt tcgcgaaaca atgcaaggta
     4801 atccggagta tggtatggtt gatatgacct cacctgatgt atttaagtcg ccttattatt
     4861 accttcatcc taatgatatt gtctatgtca acccaagaaa aaataaaatt gcttcaggtg
     4921 atttgttctt cagaattgca cctatactca tcagcctggc tactttagtt tcattattag
     4981 ttattagatt ataatttttt ttaattaagc aaagcccgct attattcaat gcaagacgaa
     5041 tttaaacaaa taagtcccga acagcaaagt ttcaatttga aacaattcat ttacgagaaa
     5101 atattctatt attggtatat ttatattcta tgtatgattt tggcattagt tacggccaac
     5161 tattacctct ggtatgtttc tcccgtttac acatctacca cttcggttat gctcgacata
     5221 gatgaaaaaa agttggccag tcaggattta ttagcaagtt tgggttctat agaaaacact
     5281 ggaggtattg aaagtcaaat gcagattatc aaatcaagaa atattatcac cagaaccatt
     5341 caacaacttg attttagatt ctcctatttt ctggaaggag atattaaaac atcagagtta
     5401 tataaaaact gtccttttga agtaattact gactcattaa cgattaccaa taatatccct
     5461 gatttgacca taagagtcac tagtgtatcc acttttgata tttcttatat caacccaaaa
     5521 actaattcgt ctcatactaa ctctcttgtt tttaataccc cctttactac tgactttgga
     5581 atatttacat taaaactaaa agaacctaaa attttaaaaa tatataaaaa ttcgattaac
     5641 gaaaaaaatg aatatattat taaagccaaa actaccgctg atttagtgca gtactacagt
     5701 tcccgtttaa atgtaacgca gattaaaggt acacgtgtac tcatgatttc tgttgatgat
     5761 gctgttccac aaaaaggctg tgatttcctt aataaactta ctgacgttta tctggatttt
     5821 ggtattgaac aaaaaaatga agataccgaa aattcattac gctttattga tggtcagtta
     5881 aagattatca ctaatgaact tttaaattcg gaaggcaaat tagaaaaata caaaacagag
     5941 aaaggtttaa ttgatttacc aggcatgtta actattgcgc aagaagatgt ctcacgtatt
     6001 gaaaatgaaa tttcgactat tgaaataaaa caaagtgtat taaaatacct taatgactac
     6061 attagaaatg gaaataatgt aaaatacctt gctcctgctt atgtagaagt tactgaacct
     6121 attttacaaa atctgattgc caatcttagt gcattacaat ctgaccttat tcagtcttta
     6181 aaagtcacta ctgttgataa tcccataatt cagaaaaaga ctattcaaat tgaaaatgta
     6241 aaagctgaaa tactagagaa gataaaaagc ataagtgatg caagtattat cactaagaat
     6301 gaaatctatc accaactgaa caagttaaag tctaaactta gtgaaatgcc aaaggcggag
     6361 agggaacttt ccaacattgc aaggcaagca aatattactg aacaactcta tatctatctt
     6421 ttacaaaaac gtgcagaaac agctattatc ttggcctcaa caatttctga cagtaaagtc
     6481 attgataatg caagaacaat tctacctcct gtaaagcctg acaaaggcat cacttatact
     6541 atttccatat tattaggact tttcattcct gcaagcattg tgtattttaa agaaattctg
     6601 gacgacagaa ttaaaagcag aaaaatgctt gagaaaagct ccactattcc agtactgggt
     6661 ataatcggtt taaccgttac agaaaacaat attgttgtca atctaaaacc tcactcacaa
     6721 gttgcagaag cttttcgttc tatcagaact aacttacaat actttcatgg agccaaaact
     6781 aacaatatca tcctcatcac ttcttcaatc agtgctgaag gaaaaagctt ttgtgcagta
     6841 aatctggcag caattcttgc ctccgggggt aaaaaggttg tgatgatagg ctgtgattta
     6901 cgtaagccac aacaacccgc caattttaat ttaacttcat ccacaggttt aagtaattac
     6961 ctgattgatt cagcaactgt agaagaaatt attcaacatg ctcctgattt aggtttggat
     7021 ttaatactgt ctggaccaaa gccaccaaat ccttcagaaa ttctgttgaa taaaagaatg
     7081 ggcgatttaa ttgaactcct taaagccaaa tatgattatg taattattga ttcgcctcct
     7141 attggattga taagtgatgg attggaatta tcaagatttg ctgatgttaa catttatatt
     7201 gtaagacaaa acttcaccag aaaactacat cttgacttta ttaataaaat atacatggat
     7261 ggcaagatga aaaatctgtg tattttattt aatggcgtac aactaggaag tactatttat
     7321 ggatatggat atggatatgg gtactcttac gggtatggat atgggtatgg atactacgaa
     7381 gaagataaaa aaccaaaaaa ctttttccaa aggttgattt acaaatttaa atttaaaaac
     7441 aattgataat caaatctcat tttattgttc ttagtaatga agcgtaaaat cacgataaga
     7501 aatgattttt tttcgtattt caaatacttt tttagaatac taaaagataa gtttctcatt
     7561 agtatttttc tgagcattgg agtaagtttt ttagatggaa ttggtatggc aatgtttatg
     7621 cctttgatac aatctgtcag cgatgatgga caaagtgtat ccttctcatc tgctgcttta
     7681 ggcaaattgc actattttac agatggcttg gagaatatgg gaataaattt aaacctatat
     7741 tctattttgg ttgttttaat tattcttttt acacttaaag gtgtatttcg cttttttcaa
     7801 cagtattatc aggtaaagat gtgtcagttt tacatgaaaa cagtacaggt agatttaacc
     7861 aaccatatac tcaatctaag ttatgaagga tttttgactt ttgatgcagg taaaattcaa
     7921 aatagcctta ccactgaagt aaaccgaatg cgaaatggaa tcatgcacta tctgacttca
     7981 tttcaatcta tcatcatgtt acttacatat atgcttctgg cctatttagc caactggaaa
     8041 tttgctttgt tggtttcgtt aggtgcagtt atatctaatg ttgtgtttaa aagagttttc
     8101 cgcctcacca aaagtgcttc taattcacta tctaaaaaag gcaaccagtt caattcttac
     8161 attcttcaag cagttcatta ttttaaatac cttaaatcaa cgaattattt ctcgcattac
     8221 tccaaaaaat taaaaactac tattgaagaa acagaatttt taaataaaaa aataggactc
     8281 tatcagagca taacatcaag tgtaagagaa cctgtattaa ttattgtggt ttctttagta
     8341 atactgatgc aggtaacatt ttttggtagt gggttaagca ccataatgct gagtttatta
     8401 cttttttaca gagcattaat ttacttattg tcactacagg cttcatggca aagttttatt
     8461 caaaatattg gagcatttga atcagttaat tccactttgt cacatatgaa aacattggaa
     8521 gaagtaaata caggaactaa aacatttagc ttcaaccata aaattcaact tcgaaacgtc
     8581 acccttcaat atggtaaaaa agtgatttta aacgatatca cattaaatat tgacaaaaac
     8641 aaaaccattg ctttaattgg agaaagtggt tctggaaaaa ccaccttggc caatttaata
     8701 gccacattac ttaagccaac aaagggtgat attttatttg ataacaatcc tgcaacttta
     8761 tatgatttaa aaacatacag aaacaaaata ggttatattt cacaggagcc ggtaattttc
     8821 aacgatacca tctataacaa tatcgctttt tggcaagaaa tctctccgga aaatatggct
     8881 aatttttgga aagttgccaa gctaactcat ttagaagatt ttattaaaag tttaaaagat
     8941 aaagagaata cacgacttgg agataatgga attttaattt caggaggaca gaagcagcgt
     9001 atttcaattg ctcgcgaact ttataaaaat gtagaattgc tgattttgga tgaagctact
     9061 tcatcacttg attctgaaac cgaatctatt attcaaaaaa acatagatac tttacatggt
     9121 aatttcacca ttgtaattat tgcacaccga ctatcaacaa ttaaaaatgc tgatgttatt
     9181 tatttaatcg aaaaggggaa gatagtagat tctggatcgt tcaacgaatt aattgaacac
     9241 tctaccaagt ttaaaagaat ggttgagcta caagaatttt aaactttaag cattacagtt
     9301 tttcaccttt tcattcattc ctaagaggaa attcaactct gtctaaatag ttaatcctta
     9361 acaagccttc atttctctct taaaaaagtt aaaacaaatt ataggaaaga ataaaatatt
     9421 tgcaagatta aaggagtaaa acaaaaatca atcactacac gagcctgatg taaactgcat
     9481 tgccaaaggc aaaacacata aaccttacaa gtatggctgc aaagcaagta ttgccactac
     9541 acaacaagaa aacattattg ttgcagcagt ttcctttcag gtcaacccgc acgacagtaa
     9601 aacactcgat gcaacgttcc atcagcatga acgaatgacc aacacaaaag caaaatcaac
     9661 ccatgtgggc aaagaatata gaggtacaaa aaaaattgga acacaaatca ttgcaaccga
     9721 tgcaggaaaa aacaaatcgt ctgatgaaaa acaaaaaatg ttggctatat aaaatcgtta
     9781 ttaatctaaa aaagcaattt actatctttg actggtattt tcaagtaatt ctgtaacatc
     9841 aatgaatatt actatagcgg ggggaggagc ctttgggact gcattagcca attccttaaa
     9901 gaaggaacat aaaattacaa tccacaccag aaacaaacag gttattcacg aaataaattg
     9961 ctatagaaaa aatatatcgt attttccaaa taaagttctt agcaaacaaa ttaaagcttc
    10021 tgatgactta acagttgcaa accaagctga ttttcttttt ctatgtattc cttcacatgc
    10081 aatagtatct tacataaagc tattaaatct taatagtaac tgtatcatta ttaatggttc
    10141 taaaggtttt ggcgataaag accaattaat acctgaaatt attagccatt tgatgaaaaa
    10201 caaaattgcc tcattaaaag gtccttcatt tgcaaacgaa ttgatttttg aaatacctac
    10261 ttcatttacc attgcaactt cagaacctga tacttttgaa gagattaaat cattattcag
    10321 gacagaggtg gcaattttcg attactcatc agatatcaaa ggtgtagaag tattaagtat
    10381 cctcaaaaat atatatgcaa ttattgtagg catagttgat gcttattata attcacctaa
    10441 tgtccgtttt ttggtattcg caaaagcact aaatgaaatt aaagcaattc tcgaattttt
    10501 aaatattcct aatgaaacac tttttaggta tgcaggcatt ggcgattttg gtttaacatc
    10561 attaaacgac ttaagcagga accgaacatt agggctacta atcggtaaag gattctttgg
    10621 taaaaatcca ggtaatcctg tagtattgga gggtgttaag gctattgagc actttgcagg
    10681 aaaaatagat acagaacaac agtatgattt aaaatacata cgtttgcttc agaaattatt
    10741 gaataatgaa accaatgtca aagactttgt aaacaaagta atttatacct aatagaaatg
    10801 ataacaaaaa ttgaaatatt aatcaatgca caaaagaata ataaacaaac ttatttgccc
    10861 aaattcacaa tcaaagcaat tacaggttca cgccattgaa gctctccgta ataacaatat
    10921 tatccacaat atttatgatg aaaatatttt acctgacgat gatatcaaaa ccggatttat
    10981 cttttcaaca gataaaaagt acatatactt tatttatgag tacattccta tattacttaa
    11041 tgatcaagat caagattctg cacatctaaa tcaaatatat gaatggatta aaaactcaat
    11101 tcctgatgaa tttaaagatg ctgtaactgc aaacatattg cgaatcagaa aggcaaatga
    11161 gtcaacagac ggcaactgga atcgtgagga aatgaaatat tatgatgctg ctgttgacac
    11221 tcctgagcta cgagataaaa tgttaaactc tatccaacgt gaaaacttgt ggcggatatt
    11281 tattcccaga aaagaagaaa tgataaatca ctttgagaaa aactgtaaag ggcaatggat
    11341 atttgaaatt ggatgtggaa atgcgcgaac agttgcaaga atgtttaatc ccgtaaagtt
    11401 caattataat tacattggca cagatatttc ttttaaaaga ttaatggttg ctaaaatggc
    11461 aatccccgag tctgatttca tacaggcaag tgcattaaat attccgataa agaataattc
    11521 atttaatggg gcaattagtt ttggtatgtt acaccatttg ccacgtccgg cagaggcggt
    11581 tacggaggtt aataaaaaat tattgcaagg tgccatattt tcctatcatg agccggttta
    11641 tacttcaagg caattttcag agaagcaaag tgcaatcgtt cgaaaagttt ttagaactta
    11701 tgaccatagt gatcatgata acaaaattaa tctgaaggag tctcttgcag cattaaagga
    11761 agcaaatcat caaattgttt caattgtacc ttataattcc gttttcagag ttgtttttga
    11821 agcaataata aatcgtttct acaagtcatg gcaaaaaagt aaacttgtaa tgaaatcagt
    11881 tttaattatt gataatattt tgcttaaaac aattgttcga ctgtcaaaca aacttgggcc
    11941 acaagctatt attgttgtat ccaaaaaact ctaattaata taatgaccac aggtaatagc
    12001 aaccattttg aggtaatcag aaaatatttt aaaaatggtt gtctcaacca atatgacacc
    12061 ttgttgagct tgcttcacta ccgaatacct tatttaaaaa atgccggcat aatattaaaa
    12121 ctgtaccagt caagtaagat tgctgtaaca accaattcaa aaaatattca aatcaacaat
    12181 tatcaagcaa attaccagca tgctattgca atgaagaatt atcttgataa aaaaataaac
    12241 aaatatgtta caggcgtatt tttacatggt agcttaggca caaatgagtt agttcaatat
    12301 agtgactttg atgcattaat catacttaat cagtcaacat ttgattcaaa aaaatctata
    12361 attaatgttg ctacaacatt aaaaaagtct gaaagaataa tgcagaaaat ggacccctta
    12421 caacatcatg gatggtttgt tctcactgaa aaagaacttg atgattttcc aactagctat
    12481 tttccacctt cattgtttga gcaagctaag tgtttaatgg gtaaaaataa atttcaaatt
    12541 catttccaga atccatcaaa tgtcaccaaa ttacaattat ttaaatcttt ctgcaatcat
    12601 ttattaagta atatcaatag aattgaacaa aatttaaact attacgaagt taaaagttta
    12661 ttgagtggat tcatgctttt accgtccgtt tacttgcagt taaacagcct ggaaggagta
    12721 ttcaaaaaag aaagttttaa tttgctcaag ggtactttat gtcaagagga atatgaaatt
    12781 atgaatcagg tttcagaaat aagggcaaac tgggattatc agccaccaat attgtataaa
    12841 gttattctct cgttaaattc atttgcatta aaccattttt taaaaaagaa aacttcaggt
    12901 aaaataccga ccaaaatcaa gaacaagctg ctagatatga gaccggcaat gacaaatttt
    12961 attcaaacac ttctaactaa agcaaagcat gaagtacaaa ttgattgata aaccacattt
    13021 tgtatcacat gaaatttata atgaagtaac agataaactt atttctaaac tggcttcgaa
    13081 tgcaaatgtg attgctattt atacaattgg acatgttgga aatcccggaa tctctgacat
    13141 tgatatgctg attattgtca aagataattg caaactaact cacaatttca gagcagatat
    13201 gtcagatata gaaaagtact tatttctgca tcaaccctat gcttgctctg agtcaggttt
    13261 taaaaaagct gagactttct cattctttca caattacaat ttagtgcatg gaattgacgt
    13321 cagaacctcc t
//
LOCUS       DAOQNP010000081        12988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1699_length_12988_cov_9.053738, whole genome shotgun sequence.
ACCESSION   DAOQNP010000081 DAOQNP010000000
VERSION     DAOQNP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1699_length_12988_cov_9.053738"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..459)
                     /locus_tag="PKY09_10395"
                     /pseudo
     CDS             complement(45..459)
                     /locus_tag="PKY09_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445192.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            757..984
                     /locus_tag="PKY09_10400"
     CDS             757..984
                     /locus_tag="PKY09_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015697087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNB13305.1"
                     /translation="MKCPNCTTTDLVMSERQGVEIDYCPTCRGVWLDRGELDKIIDKA
                     TANDAVITTYDKPKHYDSKYKKKHWLHDLFD"
     gene            1091..1498
                     /locus_tag="PKY09_10405"
     CDS             1091..1498
                     /locus_tag="PKY09_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13306.1"
                     /translation="MNFKPFFLLLLLVVAESCSLGKIDQQKGVDLTTQLLSDLKSENY
                     TDIDKYYMDSFNASEPTQTKIEKFKRLREAMGPIVSFEMLLAEVKKEPVSNVNQLHIK
                     YKVVCTKVTALQTFILVNDEGQPKIIFQNIENI"
     gene            complement(1499..2485)
                     /locus_tag="PKY09_10410"
     CDS             complement(1499..2485)
                     /locus_tag="PKY09_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB13307.1"
                     /translation="MNKKFLNLVGVITVITALASCVPARQYDEMKMRKATCDEENSKL
                     RSENTNYATQNKELTSSVADLKREKEKLETDTTTLGTAQRRLTSLYNELNDSYEKLLR
                     NNDRMMKDKNDETKKVIGQLQMTQEELIKKQDELTAKEKKLNELTEELKKRETKVNEL
                     EAVLNKKDEDVNALRKAVSDALVGFENNGLTVTKKNGKVYVSMEEKLLFASGSTVVDK
                     KGEEALKELGKVLEKNKDINVLIEGHTDNVPISGGTMKDNWDLSVLRATSVVRILTKN
                     ANIDPTRLTPAGRGPYLPVDTGNTAEARRKNRRIEIILTPKLDELFKVIGEN"
     gene            2648..3361
                     /locus_tag="PKY09_10415"
     CDS             2648..3361
                     /locus_tag="PKY09_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13308.1"
                     /translation="MATLNDNWITEEHIDFEYKKYMLLAYLQHVSESFTENKLYPPLS
                     ELMKHYRNVTALRDNKKQFYDSFPDRLSSADFNNFKLMYEKIVEDDSLMQEVESIILF
                     SIPQFEKHISEGKKIYDFIESKIKVSPVGIIPLQINAGYFFLSTTQNRETRVYEYQIT
                     IFENPDEKYRALCTQYICSYEKNLSQNFENIKGDLLANRKVFPNPATYSIESELMLPL
                     EETFLPLAKRTLMKFVNGH"
     gene            complement(3582..3782)
                     /locus_tag="PKY09_10420"
     CDS             complement(3582..3782)
                     /locus_tag="PKY09_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13309.1"
                     /translation="MDLLLAILISMGINVTPKDLTNKEFMLKNQSAIQHAQAILKERQ
                     TESTRITFKAAPQTNIFKQSKN"
     gene            4072..4575
                     /locus_tag="PKY09_10425"
     CDS             4072..4575
                     /locus_tag="PKY09_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNB13310.1"
                     /translation="MIEIRSATADDIPFITEVYNDAIINTTATFDTEVKSIEDRMLWF
                     KNHDGNHPIIVAENDGVLSGWASLTAWSDRCAYNGTAEVSVYVHKQHRGRGIGKALLE
                     ILILKGESKGLHYLLSRIASGNEASIHLHTIFGFRHIGIMKEVGFKFGNYVDVHLMEL
                     LFKTTAK"
     gene            complement(4556..5584)
                     /locus_tag="PKY09_10430"
     CDS             complement(4556..5584)
                     /locus_tag="PKY09_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNB13311.1"
                     /translation="MQLTAFPFQLRFRKPFVLASGTRTHTSVVYIKAVQNEITGWGEA
                     AMPPYVKETQDSVMQFVQQLHIPVLNTLDDLNLFFEKLYQTSGNYFAKAAINAALLDW
                     WTRKNNLSLSEFFGIEKNLPTPDCCYTITMGDDITHSIKQAADFKLLKIKAGFDGDVE
                     FIKEIAAFSEKAICIDANQGWVNVNVALQKTEQLKSLNIAFIEQPLPVNDWQGMQNLK
                     QQTTFTLIADESFQTIDDLKKIMDCFHGINIKLMKCGGISKAFQIVKSARQLNAKVLL
                     GCMSESSCGIATAVAIQQLFDWVDLDGPILISNNPFVGVRYNEGKIFIENNVGTGALP
                     VESTLLSF"
     gene            complement(5640..6650)
                     /locus_tag="PKY09_10435"
     CDS             complement(5640..6650)
                     /locus_tag="PKY09_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13312.1"
                     /translation="MKTLISAVIAMVAFTISTSAQSNIEIGISGGATNYFGDLGNNEV
                     LQSTSTRPGGAITIRNFLNSPAKTGYQYQPLSVEARFSWHRIGYDETKSIGDKKGYQL
                     RNYGRGLSFRTDVYGVSTHVSYTLYANKRKPLYAQGAAMYFYAGVGIYYARPKADLFN
                     GSIDINNRYYFWSDGTIRDQDEKSGQGNIIKRDGKFETNLDEWRTEGQGYNSELGQKK
                     PYNLMHIGFPLGMGWRWGVTKNIILSAELSYIKFITDYLDDVSDRYATYEEIKANFPE
                     EYKQDLAKYISDPSGKGTNGFEGVATSQRGNHRKTDGYAFLSLEVAYRLDFNWGKIKS
                     SF"
     gene            complement(6734..8098)
                     /gene="radA"
                     /locus_tag="PKY09_10440"
     CDS             complement(6734..8098)
                     /gene="radA"
                     /locus_tag="PKY09_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNB13313.1"
                     /translation="MAKVKSVFVCQNCGAQSPKWMGKCSSCGEWNSYVEELITSGNSS
                     VQANYKRSSKPEKITEIELHQEQRIVLPDQELNRVLGGGLVRGSLILLGGEPGIGKST
                     LLLQLAVRLKNLKTVYVSGEESGQQIRMRAERLNSDLHSQCYLLTETSLEIILQHLNA
                     LKPDMVIIDSIQTMFHSQIESSPGSVSQIRGCATELLRIAKDSGCPVFLVGHITKEGV
                     IAGPKVLEHIVDTVLQFEGDRHHIYRLLRSHKNRFGSTAEIGIYEMQGNGLAEVSDPS
                     QIMLSQRDEALSGIAIACNLEGMRTMLIETQALVSSAVYGVPQRSTTGFDQRRLNMLL
                     AVLEKRCGFKLGAKDVFLNITGGLKVDDPAIDLAVICAILSSNNDIPLEQLTCFTGEV
                     GLSGETRPVSRIEQRIKEAEKLGFKKIYFPAQNLAAFDRGMFKHIKLYPVERVDEVFS
                     LLFG"
     gene            complement(8134..8553)
                     /locus_tag="PKY09_10445"
     CDS             complement(8134..8553)
                     /locus_tag="PKY09_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13314.1"
                     /translation="MKPAAIKIPIVALSLLLLMGCESNHFFKSEKKLKSDIVGTWDRV
                     LFGQQNEETWIFVDGAIKIYQPNRHGDSADVGTFSIDATLTSPYLKIENTTYLNQNPG
                     GDTYNHKWSIVKLDNKVLEIATESPLGGVAQIEFIKQ"
     gene            complement(8612..9109)
                     /gene="rfaE2"
                     /locus_tag="PKY09_10450"
     CDS             complement(8612..9109)
                     /gene="rfaE2"
                     /locus_tag="PKY09_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNB13315.1"
                     /translation="MDYNEIISKKILNEQALMQQLSVWRLQNQSIVFTNGCFDILHLG
                     HTQYLSQAKNYGDKLILGLNSDKSGACKKGEGRPVNNETARAFVLASLHVVDAVVLFD
                     EETPYNLIKLVQPDVLVKGADYEIEKIVGYDIVIQRGGKVVTIDLTQGYSTTALLKRI
                     NNKNF"
     gene            complement(9099..10112)
                     /locus_tag="PKY09_10455"
     CDS             complement(9099..10112)
                     /locus_tag="PKY09_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNB13316.1"
                     /translation="MKKKIISVIKYALFLVAGIGMLWLTFRNQDFTQVIQKIENVHIG
                     WLLASVVAAILAHLSRAIRWNMLITPLGYKPKLINTFWALCAGYFANLAVPRLGEITR
                     CGMLTKAEQVPFNELFGTVIVERIMDVLMLFLLLLAVAALEFHLIGGFLYDTIAAPLM
                     NKFQSLNLALVAGALVIGIAAIFTYFYFSKHSGKSKAIFEKINLLLKGVVDGIKSIAK
                     MKNKSLFIFHTLFIWVMYFFASYLCFFALDSTSMLDAKAGLFILVIGGLGMSAPVQGG
                     IGAFHFIVSRGLMLYGISETEGIVYATIVHGYQTILVIALGAIGFFVLMATGKKIKAN
                     NGL"
     gene            complement(10126..10476)
                     /locus_tag="PKY09_10460"
     CDS             complement(10126..10476)
                     /locus_tag="PKY09_10460"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNB13317.1"
                     /translation="MLITVLKSKIHRAKVTQAELHYVGSITLDENLMEAADLIENEQV
                     QILNINNGERFVTYVIKGERNSGVVCLNGPAARRVQLGDIVIVASYAMMEKEEAKNYK
                     PVLVYPDEKNQPVK"
     gene            complement(10498..11937)
                     /locus_tag="PKY09_10465"
     CDS             complement(10498..11937)
                     /locus_tag="PKY09_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNB13318.1"
                     /translation="MKLLNYINGQMVEPISQKWMDNTDPSTNTVFSQIPDSDERDVQM
                     AVDAAKQAFVGWSTMPVKKRSDMLLRIAALIEKNLQRFAEAESLDNGKPVWLAKQVDI
                     PRASANFHFYATAALHLAAETHDMENTAINYTLRQPIGIAGCISPWNLPLYLFTWKIA
                     PALASGNCVVAKPSEITPVTAFLLSQFCIEAGLPAGVLNIIHGTGPRVGSAIVSHPDI
                     PVISFTGGTKTGAEISRIAAPMFKKLSLELGGKNPNIIFDDCDFDHAVATSIKSSFSN
                     QGEICLCGSRIFIQRSIYDEFKEAFVSKIKGLKTGDPKDETNFLGAIVSQPHFEKILS
                     YIDLAREEGGVILTGGKAIQPGGRCKDGFFIEPTVFENLPYTCRTNQEEIFGPVVTLT
                     PFDTEEEVLMMANSTPYGLAATVWTTNLTKAHRVAAALHSGIVWINCWLLRDLRTPFG
                     GVKQSGIGREGGFEALKFFTEEKNICIKL"
     gene            complement(12036..12218)
                     /locus_tag="PKY09_10470"
     CDS             complement(12036..12218)
                     /locus_tag="PKY09_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13319.1"
                     /translation="MNEKYLTLSIFNNTGKLIQQKTLEMNDGNIKLNLEQEAKGVYNV
                     VLSNKKKSYNGKIVFE"
     gene            complement(12280..12813)
                     /locus_tag="PKY09_10475"
     CDS             complement(12280..12813)
                     /locus_tag="PKY09_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13320.1"
                     /translation="MGLELTSISNKLYCQDSNKKNLQLSARYSFYHNYKIMHHSPVIV
                     IIFDKHNFYFGPEYSLILKRIKGDPVDIYEKESFGINFGYRYYWGEKAKRLNVFSQLN
                     YAIFKVKYKEYQHGPPFVTEHKDLIIENTISLGVDYNIWNNFHVYSGAGIGSFAGFFL
                     ITDAFLPTSYIGLEYVF"
BASE COUNT         4005 a         2493 c         2216 g         4274 t
ORIGIN      
        1 taaatttacc taatggttcg atcccgatag ctatcgggac agcaaggggg agcaacatgt
       61 tctaccttag aattttttac aattccgaca ccctgatttc tgtattttcc tgcattcttt
      121 attagtcttt ataaaagaag cttgtgagcc ttaagaaatc tttttcgctg tacggttcta
      181 attttcttat gtttttataa acttctaacg cagtatgaac ttctaaaatg tctttttccg
      241 gacctacaac tcttttgttt tctaaatggt ggtaatctgc tcttcactta gcgcatttac
      301 ttcaatgctc aaagaagaat gaatcgtgtt aatttgattc tgttttctta aagccggatt
      361 ggctttgaat aaatattttg catttacttc tccaattttt tctgaaatcg aagtaatgta
      421 ttttaaaatt actgatgtaa ctttatatgg aggtttcatg gtactaatac tatcatatga
      481 tactatcata aatatataca ttaattcgaa atgggttcaa cataatggga atagttttgt
      541 gttcagtcag tctaataaaa gcgtggagta aaccaccgtt taattactta ctcaaaaatg
      601 attttgaata gtcattaaag cgcaaattgt atatttttat gagttgagaa gcttctatag
      661 aagcacccaa gtttggaaaa cttttaatac agtaaacatt tgaatatctt tgttgttgta
      721 atacattaac tttgctaata ataaaaacaa cgaaaaatga aatgtcctaa ttgtacaact
      781 acagacttag taatgtcaga acgtcagggt gttgaaattg attactgccc tacatgcaga
      841 ggtgtttggc tcgacagagg cgaacttgat aaaattattg acaaagctac agctaatgat
      901 gctgtcatca ccacttatga caagcctaaa cactacgatt ctaagtataa aaagaaacat
      961 tggctacacg atttgttcga ttaattgata atcagaaaaa gtataaattt ttcaaataat
     1021 tggtggttta taatttaaaa gaaaatcaca taaggagttt caaatattga ttagcataaa
     1081 gtatcacaca atgaatttta aaccattttt tttactgtta ctattagtag tagcagaaag
     1141 ttgttcatta ggaaagattg atcagcaaaa aggtgttgac cttacaacac aactgctgag
     1201 cgatttaaaa agtgaaaact ataccgatat tgataaatat tatatggatt cgtttaatgc
     1261 cagcgaacca acacaaacta aaattgaaaa atttaaaaga cttcgcgagg caatgggacc
     1321 aatagtttct ttcgaaatgc tattggctga agtaaagaaa gagcctgtaa gcaacgttaa
     1381 ccagctgcac attaaataca aagttgtctg tacaaaagta actgcccttc aaacattcat
     1441 tttagtaaat gatgaagggc agccaaaaat tatttttcag aatatagaga atatctgatt
     1501 agttctctcc gataacttta aaaagttcgt caagtttagg ggttaagata atttctatgc
     1561 gtctgttttt tcttcgtgct tcggcagtat ttccggtatc aacaggcaaa taaggtcctc
     1621 ttcctgcagg ggttaatcgc gtagggtcaa tattagcatt tttggtaaga attcgcacca
     1681 cacttgtagc acgtaaaaca cttaaatccc agttatcttt cattgtacct cctgaaatgg
     1741 gaacattgtc tgtatgacct tcaatcaaga cgttgatgtc tttatttttt tcaagcactt
     1801 tgcctaattc cttcaatgcc tcctcacctt ttttgtcaac aacagtactt ccggaagcaa
     1861 acagcaattt ctcctccata ctgacatata ctttgccgtt tttcttagtc acggtaagac
     1921 cattattttc aaacccaaca agggcatcac tcaccgcttt tctcagtgca ttaacatctt
     1981 catccttctt attcaataca gcctccagct cattgacttt tgtttcgcgt tttttaagct
     2041 cttctgtcag ttcattcaat tttttttctt tcgctgtcaa ctcgtcctgt tttttaatca
     2101 actcttcctg tgtcatctgt aactgaccga ttaccttttt agtctcatca tttttatctt
     2161 tcatcatcct gtcgttgttg cgaaggagtt tttcataaga gtcgttcaac tcattgtaaa
     2221 gcgaggtcag tctgcgttgt gcagtaccta atgtagtagt gtctgtctct aatttttctt
     2281 tttcgcgttt taaatctgca acagaagatg ttagctcctt attttgggta gcataattgg
     2341 tgttttcact gcgtagcttg ctgttttctt catcacaagt ggcttttctc attttcatct
     2401 catcatattg tcttgcggga acacatgaag ccaatgcagt aatgacagta atcacaccta
     2461 ctagatttaa aaatttcttg ttcattttcg ttgtgtttaa atttattgaa actttgtttc
     2521 tgaatattaa gtaaaattat aatatagtgc ccaaaattaa gagtttggcg ctttgcgttt
     2581 ttgaaagaat aggtgcgtta ggtataattt ttgaacaatt aaaccacata aaaaaagttt
     2641 aaatattatg gcaacactca atgataattg gattacagaa gaacatatag actttgagta
     2701 caaaaaatat atgttacttg catatttaca acacgtttca gagagcttta cagaaaacaa
     2761 actttatccg ccattaagcg aactgatgaa acactatcgt aatgtaactg cgctacgcga
     2821 taataaaaaa caattttacg acagttttcc ggatcggctt tcttctgccg attttaacaa
     2881 ctttaaactc atgtacgaaa agattgttga agatgacagc ctgatgcagg aagttgagag
     2941 tatcattcta ttcagtattc ctcagtttga aaaacatatt tctgaaggga aaaaaattta
     3001 tgattttatt gaatcgaaaa ttaaagtcag tccggttggt attattccct tgcagattaa
     3061 tgcgggttat ttctttcttt caaccacaca aaaccgcgaa acaagagtgt atgaatatca
     3121 aataaccatt ttcgaaaatc cggatgagaa gtatcgtgcg ctttgtacac agtatatttg
     3181 ttcgtacgaa aaaaatctgt cgcagaattt tgaaaatatc aagggtgatt tattagctaa
     3241 tcggaaagta tttccaaatc cggcaactta tagtatagag tctgagttaa tgttgccatt
     3301 ggaagaaact tttctgcctt tggcaaaacg aacactgatg aagtttgtga atggacatta
     3361 atctgaacgt atagttggct ataaaaagaa tgcagttgga attccacaaa tgagttatac
     3421 ctttaatttc caaataaccc ggcagattta tatccatcag actatgagca agtgaataat
     3481 ttgacggtta ttggaattcc catccaatct aaatttttaa aataataaag ccaccccgaa
     3541 caatcggggt ggctttatat cagtgaaaga tgttacagaa attaattttt agattgctta
     3601 aaaatatttg tctgtggggc agccttaaat gtaatgcgcg ttgactctgt ctgacgctct
     3661 tttaaaattg cctgtgcatg ttgtattgca ctttgatttt tgagcataaa ctctttgtta
     3721 gttaaatctt ttggggttac attgattccc attgaaatta agattgcaag taataagtcc
     3781 ataatttttt gttttgaatt tgtatgggta tttgcaaatc cattaccaca atatgtgttt
     3841 tctttgtctc caaattccaa ctcttactac tgtaagtttt tttaaaagat ttactaattt
     3901 ttgaaaatat aatttaacgt tttatctctg taaaaacaaa aacaaagact gttttcattt
     3961 taaaagacgt ttgtaagaat tttacctcag caatagtttt tttatctaat aatcacctcc
     4021 tattgaaaaa tatcgtaatg tttatatttt tgtattgcca gaccacaatt tatgattgaa
     4081 ataagatcag caactgctga cgatattccg tttattactg aggtttataa tgatgctatt
     4141 ataaatacaa ctgccacctt tgatacagaa gtaaaaagta ttgaagacag gatgctttgg
     4201 tttaaaaatc atgacggcaa tcatcccata attgttgctg aaaatgatgg tgtactaagc
     4261 ggatgggcgt cactgacagc ctggagcgac cgttgtgctt ataatggtac ggctgaggtt
     4321 tctgtttatg ttcataaaca acatcgaggc aggggaatag gtaaagctct tttagaaata
     4381 ttaattctga aaggcgaaag caaagggttg cactatctgc tttcaagaat tgcttcgggt
     4441 aatgaagcca gcattcattt gcataccatt tttggttttc gccacattgg tataatgaaa
     4501 gaagtaggtt ttaaattcgg aaactatgtt gatgtgcacc taatggagtt gttgtttaaa
     4561 acgacagcaa agtagattca acaggcaatg cccctgtgcc tacattgttt tcaataaaaa
     4621 ttttcccttc attatatctg actccgacaa aaggattatt actgattaat atgggtccgt
     4681 caagatcaac ccagtcaaac aattgttgta tggctacagc tgttgctata ccacaactgc
     4741 tttcactcat gcagcccaat aacactttag catttagctg tcgtgctgac ttaacaattt
     4801 gaaaagcttt tgatattccg ccacatttca tcagcttgat atttatgccg tggaaacaat
     4861 ccattatttt cttcaaatca tcaatggtct gaaatgactc atctgctatc aaagtaaatg
     4921 ttgtctgttg ctttaggttc tgcatacctt gccaatcgtt aacaggaagt ggttgttcaa
     4981 taaaagcaat attcaaactt ttcagttgct ctgttttttg taaagcaaca tttacgttta
     5041 cccaaccctg attagcatct atgcaaatcg ctttttcact aaatgcagca atttctttta
     5101 taaactcaac atcaccatca aagcctgctt ttattttaag caacttaaaa tctgctgctt
     5161 gcttaattga atgtgtaata tcatcaccca ttgtgatggt ataacagcaa tcaggcgttg
     5221 gcaaattttt ttcaattcca aaaaattcag aaagggatag attatttttt cttgtccacc
     5281 agtcgagtaa ggctgcgttg atggctgctt tggcaaaata atttccagat gtttgatata
     5341 gtttttcaaa aaataaattc aaatcatcta aagtgtttaa tacaggaata tgcaattgtt
     5401 gaacaaactg cattacgcta tcttgtgttt cttttacgta gggtggcatg gcagcttcgc
     5461 cccaaccggt aatttcattt tgtacagctt taatataaac tactgatgtg tgagttcttg
     5521 ttcccgaagc taaaacaaat ggcttcctaa aacgaagttg aaaaggaaaa gctgttaact
     5581 gcattgctgc aaaaatgcaa caatgattaa acgatacaaa atgtgtaaag taaattttct
     5641 tagaaagatg attttatttt tccccagtta aaatcaagtc tgtaagcaac ttcaagagaa
     5701 agaaatgcat atccatctgt ttttctgtgg ttgccacgtt gtgatgttgc aacaccttca
     5761 aaaccatttg tgcctttacc ggatgggtca gagatatact ttgccaaatc ttgcttatat
     5821 tcttcaggga agtttgcttt tatttcttcg taggtagcat agcggtcact tacatcatct
     5881 aaataatcgg taataaattt aatatagctc aactctgccg ataagataat attcttagtc
     5941 acaccccagc gccatcccat tccgagcgga aaacctatgt gcataagatt gtatggtttt
     6001 ttctgcccca actcactgtt atatccttgt ccttctgtgc gccattcatc aagattggtt
     6061 tcgaacttac catcacgctt tattatattg ccttgtccgc ttttttcatc ctgatctctg
     6121 atggtgccat cactccaaaa ataatagcgg ttattgatgt caatgcttcc attaaataaa
     6181 tctgcttttg gtcgtgcata ataaatacca acaccggcat agaaatacat agctgctccc
     6241 tgtgcatata gaggctttct tttattggcg tacaaggtat agcttacatg tgttgacaca
     6301 ccataaacat cagtcctgaa acttaaaccc ctaccgtaat tacgcagttg gtaacctttt
     6361 ttatcgccaa tagattttgt ttcatcatag ccgatgcgat gccagctaaa acgtgcttca
     6421 acagataatg gctgatattg ataacccgtc ttggcagggc tatttaaaaa gttgcggata
     6481 gtaattgcac caccgggtcg tgttgaagtg gattgcagta cttcattatt acccaaatca
     6541 ccaaaataat ttgttgcacc acctgaaatg ccaatttcaa tgttgctttg tgctgatgtg
     6601 ctaatggtaa atgcaaccat agctataact gctgaaatta aggttttcat tgtttttcta
     6661 tttaaagaat gaaatattta ccgactaaaa tataatggat tttttgaata aagaagtctt
     6721 ttcagaaaaa aaactatccg aaaagcaatg aaaaaacttc atctacccgt tcaaccggat
     6781 aaagtttaat gtgcttaaac atacccctat cgaatgcggc aaggttctga gcagggaaat
     6841 agattttttt gaaacccagt ttttcggctt cctttattct ttgttcaata cggcttaccg
     6901 gacgtgtttc acctgacaac ccaacttcac ctgtaaagca tgtcagctgc tcaagaggga
     6961 tgtcattgtt acttgaaagt atggcacaaa taacagctaa atcaattgca ggatcatcaa
     7021 cctttaaccc tccggtaatg ttgagaaaaa catctttggc acctaactta aacccacatc
     7081 ttttttcaag cactgcaagc aacatattta gtcgcctttg atcaaagcct gttgttgagc
     7141 gttgtggtac accataaact gcactgctta ccaatgcctg tgtttcaatc aacatggtgc
     7201 gcatgccttc aagattacag gcaatggcaa tgccactgag tgcttcatct cgttgtgaaa
     7261 gcataatctg tgacgggtcc gatacttctg ccagaccatt accctgcatt tcgtaaatac
     7321 caatttctgc cgtagaacca aaacgatttt tatgactccg caacaaacgg taaatatgat
     7381 gtctgtcacc ttcaaactga agcaccgtgt ccacaatatg ttccaaaact ttgggtccgg
     7441 caattacacc ttctttggtg atgtgaccta ctaaaaaaac cggacatccg ctgtcttttg
     7501 caatccgcag cagttctgtt gcacatcctc gtatttgcga aacacttccg ggtgatgatt
     7561 caatctgtga atgaaacatg gtttgtatag aatcaataat aaccatgtcg ggtttcaatg
     7621 cattgaggtg ttgtagaatt atttcaagtg atgtttctgt cagaagataa cattgactgt
     7681 gcaggtctga atttagtcgt tcggcacgca ttcgaatttg ctgaccgctt tcttctccgg
     7741 aaacataaac tgttttcaga tttttcaatc ttacagcgag ctgaagtaaa agtgtagatt
     7801 ttcctattcc gggttcacca ccaaggagaa tgagagaccc tcgtaccaat ccgccaccaa
     7861 gaacacgatt taattcctga tcaggcaaca caatgcgttg ctcctgatgc agctctattt
     7921 ctgtaatttt ttcaggcttg ctgcttcgtt tgtaatttgc ctgtacagaa gaatttccgg
     7981 aggtgataag ttcttcaaca taagaattcc actcaccaca tgacgagcac ttgcccatcc
     8041 acttgggtga ctgtgcgcca caattctgac aaacaaaaac cgattttact ttagccatta
     8101 cagcgtctga aaaaaaatga tgacgctaag atatcactgt ttgataaatt caatctgtgc
     8161 tactcctccc aacggacttt cagtggcaat ttccaatact ttattatcaa gttttactat
     8221 tgaccattta tgattgtagg tatcgccacc gggattttga tttaaatagg ttgtattctc
     8281 tatctttaaa tagggtgatg ttaatgtagc atctattgag aatgtgccaa catcagcaga
     8341 atctccatga cgattgggct gataaatttt tattgcacca tcaacaaaaa tccatgtttc
     8401 ttcattctgt tgtccgaata atactctatc ccaagtacca acaatatcac tttttagttt
     8461 tttttctgat ttaaagaaat gattgctttc acaacccatt aatagtagta gtgataaggc
     8521 aacaattgga atttttatag cagccggctt cataagttct ataaattgaa aggtaaattt
     8581 acaacaaacc tatcaaattc aaaaacttta tttaaaaatt tttattgttt atgcgtttta
     8641 gcagggctgt agtggaatat ccttgtgtta aatctattgt aacaaccttg cctccacgtt
     8701 gtatgacaat atcgtagcca acaatttttt caatttcata atcagcacct tttaccagca
     8761 catcaggttg aacgagtttt atcagattat agggggtttc ctcatcaaat aatactacgg
     8821 catcaacaac atgcaaagaa gcaagtacaa aggcacgtgc tgtttcatta tttacaggtc
     8881 ttccctcacc ttttttacat gcacctgact tgtcggagtt gaggccaaga attaatttat
     8941 cgccataatt ttttgcctga gataaatatt gtgtatgccc caaatgaagg atgtcaaagc
     9001 agccattggt aaaaacaata ctttgatttt gcaatcgcca aacactaagc tgctgcatta
     9061 atgcctgttc atttagaatt ttcttgctga ttatctcgtt ataatccatt gttggcttta
     9121 atttttttcc ctgttgccat taaaacaaag aatcctattg cacccaaagc tattactaaa
     9181 atggtttgat agccatgaac aatagttgca tatacaattc cttctgtttc agaaatacca
     9241 taaagcatca gtccgcgcga aactatgaag tgaaaagccc cgatacctcc ttgcacaggt
     9301 gccgacattc ccaatccacc aataacaaga ataaagagtc ctgctttggc atcgagcata
     9361 gatgttgaat ccaaggcaaa aaaacacaaa taggaggcaa aaaaatacat cacccaaata
     9421 aataaagtgt ggaaaataaa cagcgacttg tttttcattt tcgctattga ttttattcca
     9481 tcaacaaccc ctttaagcaa taaatttatt ttttcgaaaa tggctttact ctttccggaa
     9541 tgtttagaga aatagaaata ggtaaaaatt gctgcgatgc caataactaa agcgcctgct
     9601 actaaagcaa gatttaagct ttgaaattta ttcatcaagg gtgcagcgat ggtatcatac
     9661 aaaaaaccac caataagatg aaactccaat gctgcaacag ctagcaataa caaaaacaac
     9721 ataagaacat ccatgatgcg ttccacaatc actgttccga acagttcgtt aaacggaact
     9781 tgctcggctt tagtgagcat gccacagcgt gtaatttcgc ccagacgtgg cacagccaga
     9841 ttggcaaaat atcctgcaca caatgcccaa aaggtattaa tcagttttgg cttatagcct
     9901 aaaggtgtaa tgagcatatt ccatctaatg gctctactca ggtgtgcaag tattgccgcc
     9961 acaacagatg ccagcagcca gccgatatgc acattttcaa ttttctgaat tacttgtgta
    10021 aaatcctgat tacggaaggt aagccaaagc atacctattc cggcaaccag aaacagtgca
    10081 tacttgatta ctgaaataat ttttttcttc aacctttgtc tgaatttatt ttacaggttg
    10141 atttttttca tcaggataaa ccaaaacagg tttatagttt ttggcttctt ctttttccat
    10201 catggcatag gatgctacaa taacgatgtc gcctaactgc accctgcgtg ctgcaggacc
    10261 attgaggcaa actacaccac tgttgcgttc tcctttgata acataggtaa caaatctttc
    10321 accattatta atattaagta tctgcacctg ttcgttttca atcaggtcgg cagcctccat
    10381 caggttttca tcaagtgtaa tacttcccac ataatgcaac tcggcctggg tgacctttgc
    10441 cctatggatt ttcgatttca atacagttat aagcataatg gcggcaaaag taatatgtta
    10501 gagtttaatg cagatgtttt tttcttcggt aaaaaatttc aaggcctcaa agccaccttc
    10561 gcggccaatg ccgctctgtt ttactccacc aaatggtgtc cgcaaatcgc gtaaaagcca
    10621 acagtttatc cacacaatgc cgctatgcaa agctgctgcc actctatgtg ctttagtcag
    10681 gttggttgtc cacactgtgg ctgccagtcc ataaggggtg ctgttagcca tcattaaaac
    10741 ctcctcttct gtatcaaacg gtgtgagtgt aacaacaggg ccaaaaattt cttcctgatt
    10801 ggtacgacag gtataaggca ggttttcaaa tactgtaggc tcaataaaaa acccatcttt
    10861 acagcgacct cccggctgta tggctttgcc tccggttaaa attacacctc cttcttctct
    10921 ggcaaggtca atgtatgaaa gtattttttc aaaatgtggt tgtgatacta ttgctcctaa
    10981 gaaatttgtt tcatcttttg ggtcacctgt tttcagtcct ttgattttgc tgacaaatgc
    11041 ttctttaaat tcatcataaa tactacgttg aataaatata cgtgaaccac aaaggcaaat
    11101 ttcgccctga ttgctgaaag aagattttat agaagtagct actgcatgat caaaatcgca
    11161 atcatcaaaa atgatattgg gattttttcc tcctaactca agagacaatt tcttaaacat
    11221 aggtgctgca attcttgaaa tttctgcccc ggtcttggtt cccccagtaa aactaattac
    11281 gggaatatcg ggatgcgaaa ctatggcact gccaacacgc ggccctgtgc catgaataat
    11341 attgagcact ccggcaggaa gtccggcttc aatacaaaac tgactaagca aaaatgctgt
    11401 taccggagta atttcactgg gttttgccac cacacaattt cccgatgcta atgcaggtgc
    11461 aattttccag gtaaacaaat acaaaggcag gttccagggt gagatacatc ctgcaattcc
    11521 gattggctga cgcaaagtat aattaattgc cgtgttctcc atgtcatgtg tttcggcagc
    11581 aagatgcaat gcagcagtag cataaaaatg aaaatttgca gatgcacgtg gaatgtccac
    11641 ttgtttggca agccacactg gtttgccgtt gtcaagactt tcggcttcgg caaaacgttg
    11701 gaggtttttt tcaatcagtg ctgctattct caacaacata tcagagcgtt tttttacagg
    11761 catagtactc catcccacaa aggcctgctt tgctgcatct acagccattt gtacatcacg
    11821 ttcatcactg tcgggtattt gtgaaaatac tgtgttggta gatgggtctg tattatccat
    11881 ccatttctga ctgatgggct ctaccatctg tccgttaatg taattcaaca atttcatgac
    11941 acgaagatag gtgtcattag aaaacagttt gtggttttaa aataaaaatt aaacaggctc
    12001 ttaattcttt actttgtttt ttttgggggg gacagttact caaacactat cttcccatta
    12061 taactcttct tcttattact caacacaaca ttataaacac cttttgcctc ctgctctaaa
    12121 ttcaatttta tattaccgtc attcatctct aatgtttttt gttgtatgag tttgcctgtg
    12181 ttattaaaaa tactcagcgt taagtatttt tcgttcacaa aatcatcagg tactaatcta
    12241 attagttatt atgtagaatt tgcagctaac tttaatactt taaaaaacat actcaagccc
    12301 aatgtagctt gtaggtaaaa aggcatctgt tattaaaaag aaaccagcaa aagaaccaat
    12361 acccgcacca gaatacacat ggaaattatt ccaaatatta tagtcaaccc ctagcgaaat
    12421 ggtattttca atgattaaat ctttatgttc tgtaacaaaa ggaggcccat gttgatattc
    12481 tttatatttt actttaaaaa tcgcataatt taattgacta aaaacgttta acctttttgc
    12541 tttctcgccc caataatatc tataaccaaa attgattccg aatgattcct tttcatatat
    12601 atcaacaggg tctcctttaa ttctttttaa aattaaagaa tattctggcc cgaaataaaa
    12661 attatgtttg tcaaatataa ttacaattac aggtgagtga tgcattattt tatagttgtg
    12721 ataaaatgag taacgcgcag ataattgaag atttttttta ttactatctt gacagtataa
    12781 tttatttgaa atagaggtaa gttctaatcc caaaattgta aagaatatta atcgttttaa
    12841 cactaattga gaattatttt atcaataata atgttatctc ctaaaaatcc ctttaagaag
    12901 tacatggaat tccaactcga tacttgtagt gtggcctgca tttgcgcatg aatagtggtg
    12961 ctacaaaaaa atgctaatgc aacaagag
//
LOCUS       DAOQNP010000082        12510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1778_length_12510_cov_9.348214, whole genome shotgun sequence.
ACCESSION   DAOQNP010000082 DAOQNP010000000
VERSION     DAOQNP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1778_length_12510_cov_9.348214"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKY09_10480"
     CDS             complement(<1..272)
                     /locus_tag="PKY09_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13321.1"
                     /translation="MKNILSGIITICLFCLNVTGQDVHFSQFDFSPLHQSAAQTGNFK
                     GDYRLTAINRMQYKSVTVPYKSLGASVDMNIAKTDEKKSWWSAGLL"
     gene            complement(276..2654)
                     /locus_tag="PKY09_10485"
     CDS             complement(276..2654)
                     /locus_tag="PKY09_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB13322.1"
                     /translation="MKSHFWKFCLVINFLFLFADSVKATHIVGGEIFYSYLGNGNYQV
                     TMYIYRDCANGVPPFDDPAAIGIYDQSYNLVQALNVYVRPDSILIPSTINNPCFIPPV
                     NICYRRATYIFNVNLAPSSGVYYIAYQRCCRNNTINNLIGPDVTGATYVGEIRASSFF
                     NNSSPRFKFLPPPFVCLNFPFVFDHSATDPNNDTIRYSLCTPLVGGDTLDPAPVPPFS
                     APPYSNVIFAPPFTVNNMLNGTPGVQPLSIDSITGVLTATPNTIGQFVIGVCAKEYRN
                     NVYLGETRRDYQLNVVPCPSLVVAAFLNPIVSCGSNTVSFSNGSIGAISYHWDFGVPN
                     LTNDTSNAFNPVYTYPDTGTYNVTLIAYSLFNPGCADTIHGVVTVTKELITDFTSAVQ
                     ACTTVALFNDTILSSAPASFSYNWNFGNGTTSNISHPQITYGAPGNYPVTLVVTNSLG
                     CKDTVTKTINIPQFMGATASTIQNVRCKDECNGSGLVAVINNNGPYTILWSNPPGQTN
                     DTIVNLCQGTYSGTVTDSAGCISNFSLNITEPPQLTLSATATPDYCSGQCIGTATALA
                     GGGTPPYNFVWSNSSNSSSLNQLCAGNYTVSVTDSKGCTASSVTVNVLFSDSLATLNI
                     VPSIDTIYRGQSVNLSATQNTGYIYNWQPPTYLNSNSIANPVSTPLVDIEYILSITDA
                     FGCKINDTSKIVVIQYRCEEPEIYIPNSFSPNGNGVNDEIYVYGGQIKELLFRIYDRW
                     GEKVFETKQPGQGWDGFYKGKRVTPGVFVYYVEATCYDNQKFFKKGNITVIR"
     gene            complement(2810..4627)
                     /locus_tag="PKY09_10490"
     CDS             complement(2810..4627)
                     /locus_tag="PKY09_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13323.1"
                     /translation="MKIMNRSIISFLILFWFCGSAKAQTDTTKNKSVNFYQGVEQVSK
                     SSKLLRNIYRMIFRPVTSKVVEVKKKPVVQEINFSEFQCLPIGNINITVFDPFGYSLQ
                     DSVVKHQPSLINAGNFLHNKTSSSQIKKLMIIETGDEFDSLLVSESERLIRTQPYIRD
                     AIIIPHKNELNQTIDLEVRVLDNWSILIGGGMSETNTKISITEKNFAGLGHTFENSYR
                     WYYNKNADIIESSYEVPNLLGTHADAKILYHLNEREGDVKSIGIERPFYSPLTKYAGG
                     ILLEQHFVKAVYGEPSENYSERYKYNFVDAWSGYSIRLLSGNKVYQRSTRLIAALRGS
                     SVQYLSFPSAGFDSLGLLKNQNTYLAGIGFSSREYITERYVFKYGQKEDVPIGRTYGL
                     TVGYRERASSGNWYAGAMAAFGDYFSFGYLSSLVRYGTFFYNGNARNSAFEIQTVYFT
                     RQMAIGKWRLRQFLRANATLGIDRITNEFVSLENQMSGFDSRTSGTKRIFISLQTQTY
                     APYDFLGFKFGPYVIASVGFIGNQLHGFSRSQMYSVFGVGMIIKNEFLALSSFQISLA
                     YYPTIPYSGQDVFRVNPLRSTDFQLVDFDLKKPDIIEFK"
     gene            complement(4704..5897)
                     /locus_tag="PKY09_10495"
     CDS             complement(4704..5897)
                     /locus_tag="PKY09_10495"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNB13324.1"
                     /translation="MRKLADRIERLHESQTIAMARLSRELQDQGHNIISLSLGEPDFD
                     TPKYIGDAAKKAIDDGFTKYPPISGYADVREAIAQKLKRENNLDYKASQIVVSTGAKQ
                     CIANAVLCCVNPGDEVLVPTPYWVSYSQIIQLAGGVPVYLNTTIDSDFKVTPQQVEKA
                     ITSKTRMFIFSSPCNPTGTVYSHEELKALAAVFAKHENIFVLSDEIYEYINFVGKHHS
                     IASFDELKNQVIIINGVSKGYAMTGWRIGYMAAPQDVATACDKMQGQFTSAASSIAQK
                     AAKAAISISTPDRGLMVETFRKRRDLVLNLMKDIPGWKTNTPEGAFYVFPDISYYFGK
                     SDGSSTINNPEDMCMYLLKDAKVALVTGEAFGDNQCIRFSYATSDKLLTEAIARIKVS
                     LAKLK"
     gene            complement(6004..7191)
                     /gene="kbl"
                     /locus_tag="PKY09_10500"
     CDS             complement(6004..7191)
                     /gene="kbl"
                     /locus_tag="PKY09_10500"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNB13325.1"
                     /translation="MYNTLKPVLEKELEEIRSAGLFKQERIISTPQAADITANGKEVI
                     NFCANNYLGLSSHPKVVEAAHAAINSHGYGMSSVRFICGTQDIHKTLEQKLATFLGTE
                     DTILYAAAFDANGGVFEPLLNEQDAIISDELNHASIIDGIRLCKADRHRYKHNDMADL
                     EEKLKATMNKRHRMIITDGVFSMDGTIAQLDKICDLADRYQALIMIDECHASGFMGKT
                     GRGTHEHHGVMGRIDIITGTLGKALGGAMGGFTSGRKEIVEMLRQRSRPYLFSNSLAP
                     SIVGASIAVLDMLSQTTTLRDKLWDNTQYFRQQISEAGFDIKPGQHPIVPIMLYEAQL
                     AQQFAAMLLDEGIYVTGFFYPVVAKGNARIRVQLSAGHDRHHIDKALNAFVKVGKKLG
                     VVK"
     gene            7284..8042
                     /locus_tag="PKY09_10505"
     CDS             7284..8042
                     /locus_tag="PKY09_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNB13326.1"
                     /translation="MRIVRIVLGNLWRFWFFFEAVVTFFLLFPFLLLALRGKAPYHQA
                     FKLMKFHARTIVFLSGIKVKVIRQYEPKENETYIICPNHISYLDIVLTYVAFPHYFHF
                     MGKAELERVPFFNIFFKKMNIGVNRSSRMASHKAYQRACNDLQQNISISIFPEATIPE
                     CNPQLGPMKNGAFKLAVENQINILPVCYIDNWKLLPDCPHKITGGRPGIARVIIDVPV
                     VVKGLTEADVPELKKNYRQRMENILSQYQYGYGK"
     gene            complement(8088..8327)
                     /locus_tag="PKY09_10510"
     CDS             complement(8088..8327)
                     /locus_tag="PKY09_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB13327.1"
                     /translation="MKQSDAHFEKQLKLIGRALKELRIKNGWTSYETFAVENDIDRKQ
                     YWRMEAGTNITLKTLLKVLSVHKLTLKQFFNDNKV"
     gene            8445..11324
                     /locus_tag="PKY09_10515"
     CDS             8445..11324
                     /locus_tag="PKY09_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13328.1"
                     /translation="MKQLKFIQLAFLLLALGWSTNLNAQFCPVMIEGTTTLCTYISAA
                     PGQPVVQIKNYDWLHPSYYSISASPSGSVNASIIPSTFSVADIHIGLTVNNNVTDIKI
                     TVTYNSPSCSSTVDYYLYKCCEQSGMFNYFDTDISAMPKTWNPLGFYEYNGLNVAVNG
                     VLTVNSSAQIINSNFFLAPGSVIKVKEDYYNPVNIKLMNTRFLAGCDEMWKGIELEGA
                     NSITLTNTSSIEDAQYAINFKKGGTIHIGSGGCNFNKNFVSIYAPDVSSLLPPDNIHN
                     IVVNAFDKANFSGQATTLNNYMGQSPAIINNKTLCGILLYDVANFQIPQSVTSSLRSN
                     FSFLNSGIAGIRSNIEIHNCAFNNVQPLSGYSAYTYSGSAVFNLNNTTNYMTTTIGKL
                     YGNYSDNLFTNCYRATYTTGNINATIRGNDYNVIAQEAAFINNCPGCNITITNNKIVA
                     TNMGIKILSSTDALLSINNNNISSVFPYNSNSFGNTAIQIYNKSKVPVRGVINNNTIS
                     NCRIGIYLSNLDKNKNINVPKLEITNNPMYFNLAPNLIGQSMHYAIWADGCNNLNIHD
                     NQNIYRSHAISTAPANFDQTMRGIYIRKGQSIDLKQNDITNFGTAIRLQDNCSNTQLQ
                     CNNMYSCLQGVFLNDGTTIVTTQGNPATGQAWDNKWVNFGTNNRTACPALAFPPPVQW
                     LHQGTAMPGSSNYSIFSPFPTNGTFVNAVQNSGVGPCVNNYAGDDEESMLNHIDDVIA
                     ETVDYDGTYETEDEYKDRQAAFEYLHEHESYRNSDAGLLAFYNQTLQENIGEFSNVEM
                     LMRAGDYVNALMELNGILVENLIEQYRAYTLEIAIRMEENPEYELSQAEIDELLNIAY
                     TPLYIGGEGVLNSRAILGLEVDDQAMSLRVMNPHNNESTKTLAVQTLDETVLKTLVYD
                     NIGAKVLEGSKFTNADLRKALPSGVYIIKTIKQSTSSTKKVSIIK"
     gene            11376..>12510
                     /locus_tag="PKY09_10520"
     CDS             11376..>12510
                     /locus_tag="PKY09_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13329.1"
                     /translation="MEKRALLFFFFLFSYNTYAQQNNIWCFGDSAGIDFNASLPSTFN
                     SSIKGRGSCTSIADSTGNLLFYAYTRATLFGKTTLVFNYTHQLMDNGDSIVGEGWYNE
                     LTIIPDPVNPKRFYMISVGVLDLALQQGFYYSIIDMSYNGGLGKVVSKNNQLLNNFMV
                     DCIGAIKHGNGRDWWVVCRKGDFYIGGPAHNEYTFYLITPTGVSVPITKSVGSLTSYN
                     LANLIFSPTGNKMIYTNPRGLLELYDFDRCTGDLTLDQTISPENTPYHFYWSSAFSPD
                     ESKLYVQTVSYPDTTYLIQFDLNAANIAASADTIFTQPLATATGGDVRLAPDGKIYVS
                     NAYYDGFNFAFPYSDTTYNMYNSYLGVVNYPDSMGADCDFQPYS"
BASE COUNT         3922 a         2416 c         2277 g         3895 t
ORIGIN      
        1 aataacaacc ctgcactcca ccatgatttc ttttcatcag tctttgcaat gttcatatca
       61 acagatgcac ctaacgattt gtaaggcact gttacagatt tatattgcat gcgatttatt
      121 gcagtcagtc ggtaatcacc tttaaagttt cctgtttgtg ctgcactttg atgcagtggt
      181 gaaaaatcaa actgcgaaaa atggacatcc tgccctgtta cattcaaaca gaacaaacaa
      241 attgtaatta tgcccgacag gatgtttttc attacttacc gaattacggt tatattaccc
      301 tttttaaaga atttctgatt gtcataacaa gtagcctcaa catagtaaac aaacacccct
      361 ggtgttacac gtttgccttt ataaaaacca tcccaacctt gtcccggttg tttggtctca
      421 aatactttct ccccccaacg atcgtaaata cggaatagca actcttttat ttgtccgcca
      481 taaacgtaaa tctcatcatt cacaccattg ccatttgggc tgaatgaatt gggaatataa
      541 atttccggct cttcgcatcg gtattgaatc actacaattt ttgaagtgtc attaatctta
      601 caaccgaatg catctgttat gctaaggata tattcaatat caacaagtgg tgtacttaca
      661 ggatttgcaa tactgttgct atttaaataa gttggtggct gccagttgta gatgtagcct
      721 gtattttggg ttgctgaaag attaacactt tgtccgcgat aaatagtatc aatagaaggc
      781 acaatattta atgtcgccaa cgaatcagaa aacaaaacat ttaccgtaac agaagaagct
      841 gtacaacctt tcgagtctgt tacagaaact gtgtagttgc cggcacaaag ttgattgagc
      901 gaactcgaat ttgagctatt gctccagaca aagttgtaag gcggtgtgcc tccacccgcc
      961 aatgctgtgg cagtgcctat acattgaccg ctgcaatagt caggagttgc agttgcagat
     1021 aaggtaagtt gtggtggttc agtaatattt aaagaaaaat ttgaaataca tcctgcagaa
     1081 tctgttactg ttcctgaata ggttccctga catagattaa caattgtatc attggtttgt
     1141 cccggaggat tgctccataa tattgtgtat ggcccattat tatttattac tgcgacaagg
     1201 ccggaaccgt tacattcgtc tttgcaacgt acattctgaa tggtggatgc cgttgcaccc
     1261 atgaattgtg gtatgttaat ggttttagta actgtatcct tacaaccgag tgagtttgta
     1321 accactaagg taaccggata attacccggt gcgccatagg taatttgtgg atgacttata
     1381 tttgatgttg tgccattacc aaaattccag ttataggaaa atgaagcagg tgcagatgat
     1441 aaaattgtgt cgttaaataa ggctacagtt gtacatgcct gaacggcaga tgtaaaatct
     1501 gttatcagtt ctttagttac tgttacaaca ccatgaatgg tgtcggcaca acccggatta
     1561 aataaagaat aagcaattaa cgtaacatta tatgttcctg tatcgggata ggtataaaca
     1621 gggttaaatg cattagatgt atcattagta agattaggta caccaaaatc ccaatgataa
     1681 gaaatagcac ctatactacc attactaaat gaaactgtat tacttccaca agaaactatt
     1741 gggtttaaaa atgctgcaac caccaaagac ggacagggaa ctacgtttaa ctgataatct
     1801 cttcttgttt cgcctaagta aacattgttg cgatattctt ttgcacagac accaattaca
     1861 aactgaccta ttgtgtttgg ggtggccgta agcactcctg ttattgaatc aatactcagc
     1921 ggttgaacac ccggagtacc attgagcata ttgttcaccg taaatggcgg tgcaaatatt
     1981 acattactat atggtggtgc agaaaacggt ggaactggtg ccggatccaa ggtgtcgcca
     2041 ccaactaagg gcgtgcataa tgaataacga atggtatcat tattaggatc tgtagctgaa
     2101 tggtcgaaaa caaatgggaa atttaaacaa acaaaaggtg gtggcagaaa tttaaaacgc
     2161 gggctgctgt tgttaaagaa actgcttgct cttatttcac caacataggt tgcaccggtc
     2221 acatccggtc caattaaatt gttgatggta ttattacgac agcaacgctg ataggctata
     2281 tagtaaacac cggaagaggg tgccagatta acattaaaaa tataggtggc acgcctgtaa
     2341 caaatattta caggtgggat aaaacatgga ttgttgattg ttgaaggaat aagaatagag
     2401 tcaggtcgaa cataaacatt caatgcttgc actaaattgt aactttgatc gtaaatgcct
     2461 attgctgcag gatcatcaaa aggtggtaca ccattggcac agtcacgata gatatacatt
     2521 gtgacctgat aatttccatt accaaggtag ctgtaaaaaa tttctcctcc tacaatatgc
     2581 gttgccttta cactgtctgc aaacagaaat aaaaaattaa taactaaaca gaatttccaa
     2641 aagtggcttt tcatatatga tggaaaggta acaaagatac taaaattgat ttctaaataa
     2701 tgactacaaa atttaaaaaa tcattgtctt taaacggtaa tttccctgtt ctgaaagata
     2761 gaaacttcaa tatatactca aaacacctaa gacttactca agtatattac tacttgaatt
     2821 caataatatc aggttttttt aaatcgaaat caactaactg aaagtctgtg ctcctaagtg
     2881 ggttgactct aaacacatct tgccctgaat atggaattgt aggataataa gccagactta
     2941 tctgaaaact gcttagtgcc agaaattcat ttttaattat catcccaact ccaaaaacag
     3001 aatacatttg gcttctgcta aaaccatgaa gttgatttcc aatgaagcct actgatgcaa
     3061 tgacataagg tccaaactta aagcccaaaa aatcataagg agcataggtt tgtgtctgca
     3121 gagagataaa aattcgcttg gtgcctgagg tgcggctatc gaaaccactc atctgatttt
     3181 cgagcgaaac aaattcatta gtaattctgt caattcctaa tgttgcattt gctctcagga
     3241 attgtctaag cctccacttg cctattgcca tttgtcgtgt aaaataaaca gtctgaattt
     3301 caaatgcaga atttctggca ttgccgttat aaaaaaatgt accgtaacga actaaactgc
     3361 tcagataacc aaaactaaaa taatctccga atgcagccat ggcaccggca taccaattgc
     3421 ctgatgaagc tctttcccta tagccaactg ttaatccata ggtgcggcca ataggaacat
     3481 cttccttttg tccgtattta aaaacatacc tttccgtaat atactctctt gaagaaaatc
     3541 caattcctgc aagataggta ttttgatttt tcaataaccc caaagaatca aaacctgcag
     3601 agggaaaaga aagatactga acgctgctgc ctcttaatgc agcaatcaat cgtgtgctgc
     3661 gctgatacac cttattgccg cttaacaaac gtatagaata acccgaccaa gcatctacga
     3721 agttgtactt gtatcgctct gagtaatttt ctgatggctc accatatacg gcctttacga
     3781 agtgttgttc taataatatt ccacctgcat attttgtcag tggtgagtaa aatggtcttt
     3841 caatcccaat agacttaaca tcgccttctc tttcgtttaa atgataaagt atttttgcat
     3901 ctgcatgggt tcccagcaaa ttcggaacct cataactact ctcaataatg tcagcatttt
     3961 tgttgtaata ccatcggtag ctgttttcaa aagtgtgtcc cagtccggca aagttttttt
     4021 ctgtaatact gatttttgta ttggtctcac tcatgcctcc gccaattaaa atactccagt
     4081 tgtcaagtac gcgcacttca aggtctatgg tttgatttaa ctcattttta tgtggaatga
     4141 taatggcatc gcgtatataa ggttgtgtac gaatcaatcg ttccgactct gaaactaaca
     4201 atgaatcaaa ctcatctcca gtttcaataa tcattaactt tttgatttga ctgctacttg
     4261 ttttattatg caaaaaattt cctgcattga ttaaagaagg ttgatgtttg acaactgagt
     4321 cctgtaaaga ataaccaaac gggtcgaata cggtaatatt aatatttcct attggcagac
     4381 attgaaattc tgagaaattg atttcctgta caacaggttt tttcttaacc tcaacaactt
     4441 tggaggtaac aggtcggaaa atcattctgt aaatgttacg aagcagttta ctcgattttg
     4501 aaacctgctc aactccctga taaaaattaa cagatttatt ttttgttgta tctgtttgtg
     4561 cctttgctga accacagaac caaaataaaa tcagaaaaga aataatactg cggttcatta
     4621 ttttcattga ttcaaaaata aattgcgtat gcaattatgg tttacgaaaa taacaataag
     4681 ccccgaacta aaaagaataa aaactatttc agttttgcca gactaacctt tatgcgggca
     4741 atagcttcgg tgagcagttt atcgctggta gcataagaga aacgaataca ttgattgtcg
     4801 ccaaatgctt caccggttac taaggcaact tttgcatctt tcagcagata catacacata
     4861 tcttccggat tatttatggt gctgctgcca tctgattttc cgaaataata actgatgtcg
     4921 ggaaaaacat aaaatgcacc ttctggagtg ttggttttcc aaccgggaat atctttcatc
     4981 aggtttaaaa ccaagtcgcg ccttttgcga aaagtttcta ccattaaacc tctgtcaggt
     5041 gtactaatgc tgatagctgc ttttgctgcc ttttgtgcaa tactgcttgc agccgatgta
     5101 aactgtcctt gcattttatc acatgcagta gctacatcct gtggtgctgc catatagcca
     5161 atacgccatc ctgtcattgc atagccttta cttacaccat ttattatgat gacctgattt
     5221 tttaattcgt caaatgatgc aatgctgtga tgctttccaa caaagttgat gtattcatag
     5281 atctcatcac ttaacacaaa aatattttca tgctttgcaa aaactgctgc aagtgctttt
     5341 aactcctcgt gcgaataaac agtacctgtt ggattacatg gtgatgaaaa aataaacatc
     5401 cttgtttttg atgtaattgc tttttcaacc tgctgtggtg ttactttaaa atcagaatca
     5461 atagtggtat tcaaataaac aggtactcct cctgctaatt gaataatctg tgagtagctt
     5521 acccaataag gtgttggcac caacacttca tcaccgggat ttacacaaca aagcactgca
     5581 tttgcaatac attgttttgc tcctgtgctg acaacaatct gagatgcttt atagtctaga
     5641 ttattttcac gctttaactt ctgagcaata gcttcgcgca catctgcata tcccgatatg
     5701 ggtggatact tcgtaaatcc gtcatcaatc gccttttttg ctgcatcacc aatatatttt
     5761 ggtgtatcaa aatcgggttc gcccaagctg agactgataa tattatgtcc ctgatcctgc
     5821 aattcgcggc tgaggcgtgc catggcaatg gtttgcgatt catgcaatcg ctctattcgg
     5881 tctgctaatt ttcgcatgtc tgatgcattc aataataggg cgcaaagcta acaatacttc
     5941 attacagaaa ataaaacaaa aagcaggtaa ttaactttga cttgttcaat tcaaaaagag
     6001 agcttactta actactccca atttttttcc tactttaaca aaggcattca atgctttgtc
     6061 aatgtgatgt ctgtcatgtc ctgcagaaag ttgtactctg atgcgtgcat taccttttgc
     6121 aacaacagga taaaagaaac cggtaacata aatgccttcg tctaataaca ttgctgcaaa
     6181 ctgttgtgct aactgtgcct cataaagcat tatcggaaca ataggatgct gaccgggttt
     6241 aatatcaaag cctgcctcag aaatttgttg acggaaatat tgtgtattgt cccaaagttt
     6301 atcgcgcagt gttgttgtct gacttagcat atcaagtact gcaatagatg ctcccacaat
     6361 agaaggtgcc agggaattgg aaaacaaata aggtcttgaa cgctgacgca acatttcaac
     6421 aatttctttt ctgcctgaag taaagcctcc cattgcacca cccaatgcct ttcctaaagt
     6481 tcctgtaata atatctatac gacccattac gccatgatgc tcatgtgtgc cacgtcctgt
     6541 tttgcccata aaacctgatg catgacattc atcaatcatg atcaatgcct gatagcggtc
     6601 ggccaggtca cagattttat ctaactgcgc tatggtacca tccatgctga atacaccatc
     6661 ggtaataatc atgcggtgac gcttgttcat tgtagctttt agtttttctt ccaggtctgc
     6721 catgtcgttg tgcttgtagc gatgacggtc tgccttgcaa agtctgatac catcaatgat
     6781 agaggcatgg ttcagctcat cagatataat agcatcctgc tcattgagca aaggctcaaa
     6841 cacaccacca ttggcatcaa atgcagctgc ataaagtatg gtgtcttcag tgcctaaaaa
     6901 cgttgctaac ttttgttcta aggttttgtg aatgtcctgt gtgccgcaaa tgaaacgcac
     6961 actcgacatg ccatagccat gagagttgat tgcagcatgt gccgcttcaa caacttttgg
     7021 atgcgatgat aaaccgagat aattattagc acaaaaatta ataacttctt ttccgtttgc
     7081 tgtaatatca gcagcttgtg gtgtggaaat aattctttct tgcttaaaca atcctgctga
     7141 cctaatttct tcaagttctt tttccagtac gggttttaat gtgttgtaca tatcatgaaa
     7201 ttttaatgca aagtaataaa atagaattta cttgaaccta atgcaataaa aacttaacgc
     7261 aggtattttt atactttagc agcatgagaa ttgtgcgcat tgtgcttgga aacctgtggc
     7321 ggttttggtt tttctttgaa gccgttgtta ctttctttct gttgtttcca ttcttattac
     7381 ttgcattgcg tggcaaagcc ccctatcatc aggcatttaa actgatgaaa ttccatgcac
     7441 gtaccatagt atttctttcg ggaatcaaag taaaagttat tcgccaatat gaacccaagg
     7501 agaatgaaac ttatattatc tgtccgaatc atatttctta tttagatatt gtgttgacat
     7561 acgtagcatt tccgcactat tttcatttta tgggcaaggc cgagttggag cgtgttcctt
     7621 tcttcaatat tttttttaaa aagatgaaca ttggtgtaaa ccgttcgagc cgcatggctt
     7681 cgcacaaagc ctatcagcgt gcctgcaacg acctgcagca aaacatcagc atctctattt
     7741 ttccggaagc caccattccg gaatgtaatc cacaattagg ccccatgaaa aatggtgctt
     7801 ttaaattagc agtagaaaat caaatcaata ttttgcccgt ttgttacatt gataactgga
     7861 aactgttgcc cgattgccct cataaaatta ctggcggaag accgggtatt gccagagtga
     7921 ttatagatgt acccgttgtg gtaaaagggc tgacggaagc cgatgtgcct gagttgaaaa
     7981 aaaattaccg tcaacgaatg gaaaacatac tttcacaata tcaatatggg tatggtaaat
     8041 aaccatagga aaaagcaaag ttgaaaagca ataattcctt tttctaatca caccttatta
     8101 tcattaaaaa actgctttag cgttaattta tgaaccgata atacttttaa cagcgttttt
     8161 aaagtaatat ttgtgcctgc ttccatacgc cagtattgtt ttctgtctat gtcattttca
     8221 actgcaaatg tctcatagct tgtccaacca ttcttaatgc gcaattcctt taaagcccgt
     8281 ccaataagct ttagttgttt ttcaaaatgc gcatcacttt gcttcattat aaaagcaatg
     8341 atgctgtttg ttagataaat aaattacggc atatattgca cattttcttt tttttatcta
     8401 catttgaagc ataaattaat cttcacttat taacacctaa atttatgaaa caactaaaat
     8461 ttattcaatt agcatttttg ctactcgcat taggctggag tactaacctt aatgctcaat
     8521 tttgtccagt aatgattgaa ggtacaacga ctttgtgtac atacatctct gctgcaccgg
     8581 ggcaacctgt agttcaaata aaaaattatg actggctgca cccatcctat tactctatta
     8641 gtgcctcgcc ttcaggatca gtaaatgcct ctatcattcc ttctactttc agtgtggcag
     8701 atattcatat aggattgact gtaaacaaca atgttacaga tattaaaatt acggtgacat
     8761 ataactcccc tagttgtagc tcaacagtag attattattt gtataaatgt tgtgagcaat
     8821 caggaatgtt caactacttt gatacagata tcagtgcaat gccaaaaaca tggaatccgt
     8881 taggctttta tgaatataat ggtttaaatg ttgccgttaa tggagtacta accgtaaact
     8941 cctcagcaca aataatcaat agtaattttt ttttggcacc tggctctgta ataaaggtta
     9001 aggaagatta ttataaccct gtaaacatta aattgatgaa tacacgtttt ttagccggct
     9061 gtgatgaaat gtggaagggc attgagttag aaggtgcaaa tagtattaca ctaacaaata
     9121 caagttctat agaagatgct caatatgcca ttaactttaa aaaaggtggt acaatacata
     9181 tcggatctgg cggatgtaat tttaataaaa attttgtttc tatttatgca cctgatgttt
     9241 catcactact gcctcctgat aacattcaca atatagtagt taacgcattt gacaaagcta
     9301 acttttctgg acaggctact acactcaata actacatggg tcaatcgcct gccataataa
     9361 ataataaaac actttgcgga atcttgttat acgatgtagc caattttcaa attccacaat
     9421 ccgttacatc ttctttaaga agtaattttt catttttgaa ttcaggaatt gccggcattc
     9481 gctccaacat agaaatacat aattgtgctt ttaataatgt acaaccttta tcaggttata
     9541 gtgcttatac ttatagtggt agtgctgttt ttaatttaaa taataccact aactacatga
     9601 ctactacaat tggcaaatta tatggtaact attctgataa tctatttacc aattgctacc
     9661 gtgccactta tacaaccggc aatatcaatg ctaccattag aggtaatgat tataatgtaa
     9721 tagcacaaga agctgctttt attaataatt gtcctggctg taatattact ataacaaaca
     9781 acaagatagt tgcaacaaat atgggcatta aaattttatc cagcacggat gccttattat
     9841 ctattaacaa caacaacatt tcatccgttt ttccatataa ctccaacagt tttgggaata
     9901 ctgccataca gatttacaat aaaagtaagg ttcctgtacg tggtgtaata aacaataaca
     9961 ccattagtaa ctgccgcatt ggaatttatc tctccaatct tgataaaaat aaaaacatta
    10021 atgtgcccaa gctggaaatc actaacaatc ccatgtattt taatctggca cctaatttaa
    10081 ttgggcaaag tatgcactat gcaatatggg ctgatggatg caacaacctg aacattcatg
    10141 acaaccaaaa catctataga agccatgcaa ttagtacagc gccagccaac tttgaccaaa
    10201 ccatgcgtgg tatttatatt cgtaaaggcc agagtattga cttaaaacaa aacgacatta
    10261 ccaacttcgg tacggccata cgcttacagg ataattgcag taacacacaa ctgcaatgca
    10321 acaacatgta ctcttgttta cagggagtgt ttttaaacga tggtactact atagtgacaa
    10381 cacaaggaaa ccccgccaca gggcaggcat gggataataa atgggtaaac tttggcacta
    10441 ataacaggac ggcttgtccg gctttagcct ttcctccacc tgtgcaatgg cttcaccaag
    10501 gaactgctat gcccggttca tccaattata gcatttttag tccattccca actaatggaa
    10561 cttttgttaa tgcagtacag aacagtggtg taggtccctg tgtaaataat tacgctggcg
    10621 atgatgagga atccatgctc aatcatattg atgacgtaat agccgagacc gtggattatg
    10681 atggcaccta tgaaacagaa gatgaatata aagacaggca ggcagcattt gaatatttac
    10741 acgagcatga aagctaccgc aacagcgatg cagggttgct ggctttttac aaccaaacgc
    10801 tgcaggaaaa cataggtgag tttagcaatg tggaaatgct gatgcgagca ggtgactatg
    10861 taaatgcact gatggaatta aacggtatat tggttgagaa cctgatagaa caatacaggg
    10921 catacacgct tgaaattgcc atacggatgg aagaaaatcc tgagtatgaa ctgtcgcagg
    10981 cagaaataga tgagttattg aacattgcct atactccttt gtacataggt ggcgaaggtg
    11041 tattaaattc ccgcgctatt ttgggattgg aggtggacga ccaggctatg agcttgcgtg
    11101 tgatgaatcc acataataac gaaagtacaa aaacattggc tgtacaaacc cttgatgaaa
    11161 cagtacttaa gactttggtg tatgataaca taggagcaaa agttttagaa ggtagtaaat
    11221 ttactaatgc tgatttacga aaagcattac cttccggtgt ttatattatt aaaacaataa
    11281 aacaaagcac atcaagtact aaaaaggtaa gtattataaa atagcttaat tttatgcagc
    11341 aacaggtttt acctgttgct gcatttttaa tttaaatgga aaaacgcgca ttactatttt
    11401 tctttttttt attttcttat aacacttatg cacaacaaaa caacatttgg tgttttggag
    11461 acagtgcggg tattgatttt aatgcaagtc taccatcaac ttttaatagt agtatcaagg
    11521 gtagaggaag ttgtacaagt attgctgaca gcactggcaa cttgcttttt tatgcctaca
    11581 caagggctac attatttggt aaaacaactt tagtctttaa ctacactcat cagttgatgg
    11641 ataatggaga ttcaattgtg ggtgaaggtt ggtacaacga attaactatt atacctgatc
    11701 ctgtaaaccc aaaaagattt tatatgatta gtgttggagt acttgattta gcacttcagc
    11761 aaggcttcta ttacagcatc attgatatga gctacaatgg aggcttaggc aaggtagtat
    11821 caaaaaacaa tcaactgctt aacaacttta tggttgattg cataggggct attaaacatg
    11881 gcaatggcag agactggtgg gtagtatgcc gtaaaggcga tttttatatt ggaggacctg
    11941 cacacaatga atatactttt tatttaataa cgccaacagg tgtttcagta cctataacaa
    12001 aaagtgttgg ttcactaacc agttataatc tggcaaatct aattttttcg ccaaccggca
    12061 ataaaatgat ttataccaac ccccgtggtt tattggagtt atatgatttt gacagatgta
    12121 cgggtgattt aacacttgat caaaccattt ctcctgaaaa cactccttat cacttttact
    12181 ggagtagtgc cttttctcct gacgaaagta agttgtatgt acaaacagtt tcgtaccctg
    12241 atactactta cctgatacaa tttgatttaa atgcagccaa cattgctgcc agtgctgata
    12301 ccatttttac acaaccactt gcaacggcca ctggtggcga tgtacgttta gcgcctgatg
    12361 gaaaaatata tgtaagcaac gcttattatg atgggtttaa ctttgctttt ccgtatagcg
    12421 ataccactta caatatgtac aactcatatt tgggagtagt taattatcct gatagcatgg
    12481 gcgcagattg tgactttcaa ccgtacagtt
//
LOCUS       DAOQNP010000083        12061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1871_length_12061_cov_9.696485, whole genome shotgun sequence.
ACCESSION   DAOQNP010000083 DAOQNP010000000
VERSION     DAOQNP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1871_length_12061_cov_9.696485"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKY09_10525"
     CDS             <1..747
                     /locus_tag="PKY09_10525"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNB13330.1"
                     /translation="LLFENDVTGDADYKTTIVDVSVAWNSFLDENKKHQLTLAIQPGW
                     MQNGLNFSELYFGSQYDGDIFDPNLPTGENPERSSYSNFNLNAGAGYRYAFSENSDLN
                     LVFGASHLNQPDISFLNDKAKLFTRLTGIGVLNLMLSEKISLQPQVLFYRQGKFSQLN
                     GGANVQLHSLENNLKNFRFSAGLFYRNKDAVIARIGAGIGNLDAGFAYDFNTSDLKRA
                     SNGRGAYEVALSYIITKVKPLKKNPPCPVY"
     gene            732..2759
                     /locus_tag="PKY09_10530"
     CDS             732..2759
                     /locus_tag="PKY09_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13331.1"
                     /translation="MPGLLTFVFGSRRHVVARVVMFASLFFAAQQLHAQSPAAYERAG
                     DKAMKEGDYYSAFTFYFNSYANNNTDLIITWKLAESCRLYNDYENAQKYYNKAYGLDK
                     QGQFPLIAFYLAEMNQRQGYYEEAASIYNNYLQMYGRDNSYFTDKAKRELNNCQNIKQ
                     LLKDTAKVSIQNSGTEINSVYSDFAPSYLDSTTMLYSSLRFENKDKKTKKKNKYISKL
                     LISNNERNTWSSPDNYSTQLNRPGLHSCNSAVSGNGKLLIYTQCQREKDKLMCTLYYS
                     TQKDGNWTNPLPLNDSINFKGYTATMPAIGNDGLSGYVLYFVSDRPGGLGKADIWKCS
                     ITPDFIFGNPVNVGAPVNTVDDDITPFYDNQHSTLYFASEGHKGLGGFDIYSYKTDVP
                     EQNVENLHYPINTSYNDLYYSSRNNNFLLSSNRPGSLYIKARTCCYDIYEGVYLDTVK
                     HVKKDTLMAIEPFANDISQDSITKEVIRQIEPLLPMKLYFENDQPNPKTMLTTTKANY
                     VDLYHTYLTNEKKYRVNYVDVLTDESKRTNADAEITAFFNQVVKKSYLDLDRFSDLLL
                     KQLDAGNKIEIIVRGSASPLAASGYNKNLSKRRISSVINFWKVYNAGKLNNYIKTGKL
                     TITEDAVGEEMTSTKISDDLKDKRNSVYSPAAGSMRYIQVMRVMINGVAVE"
     gene            2830..5358
                     /locus_tag="PKY09_10535"
     CDS             2830..5358
                     /locus_tag="PKY09_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB13332.1"
                     /translation="MNSEISEKVSRLREQADALMSVNTAKAIILLSEAMNQIEADDEK
                     LKSECGLQLALAYHKVKEELMAIKIINQCLELKINSDSLKNQIPFNELAAEIYTAISI
                     YDSALEHLLNIASAYTSAKDKQKLGLVLNQIGETHKLLGGYNEAIRHHEKALKIFEEL
                     ENKEQLSITNFYIGNCYNWADELDIAFNYLDTSLKLAEKLRSPELKIKTLGSLAILFT
                     KQNKFEKSLDYFFQAIDNSNLAGNVRVKADLLKSLGNLYNQMHRHDEAIKILLEADEI
                     CQSLNIKQPLHLVNLFLSDAYAALNNFEKALAHHKVYYKLCREIQSEDVSHRTKNLQL
                     KYDLEEIQREKNKAEESLLLKDTFLANVSHELRTPLNGIIGMADLLSETVATDENRKY
                     VQTILSSAFQLSNIVDEILDYARVNTGQVCPVEHPFILNETIKECLEKFLLKFPGKKV
                     ESDTDFKACDEVLLDEGILKKVVFNTLVFGGSFASNDKLELALYHSQNKIFINVKFNI
                     VNKKSFPHPDNFFELNNPQFSQNGLNGYGLGLSLINARGMIMACGGKISVKLESSKCN
                     FVIELPYKAVKSDRKNIVNEPTATEDVTILLVEDNKINQFLAKTMLQKKGYKVALATD
                     GEEALNLLEKGNFSMVITDVQMPGMDGYQLAGHIRSKLSGPVNAIPILALTAYESSAE
                     KEKAKAAGMSGFLTKPYMPEQLFDLIAQHLPLKNDKLNDAAESESSFNVNEIYENLLA
                     LMAGDKKETASLIKMLQVQFPELLSEMLQAIEDKDMKKLYKAAHKIKSSLKIFNNNSV
                     GVLIDQVERKAKDNEYSFELSVKVSELIKQVNVIIGSLDQLVKK"
     gene            complement(5369..5920)
                     /locus_tag="PKY09_10540"
     CDS             complement(5369..5920)
                     /locus_tag="PKY09_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNB13333.1"
                     /translation="MKQILMNEKLTFRVVAIISVVVLGMVMLLSQKVLPRPETPPEFT
                     KYLPGLNALVNGTCFILLLISFYFIKKKRIDIHKKINLLTFLLSAVFIVSYFLYHYLA
                     EETKYPVDHPLRGVYLFILASHIILAAIVMPLVLLSFYYGLKMDVRKHRKLTRFSFPI
                     WLYVTLTGVIVYLMISPYYSFNQ"
     gene            complement(5922..6515)
                     /locus_tag="PKY09_10545"
     CDS             complement(5922..6515)
                     /locus_tag="PKY09_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB13334.1"
                     /translation="MPLFFFILMKEGKHNFRYLEYYGPKHLATNGVDTIYHTLTDFSL
                     KNTEGNTVSLSNTDPSYWVFQFFNASCQEPCKEVFTNMYDVARDFATAPKVKLLSITT
                     HPKNDSIPVLKQVMKMNSIHPPQWQLCTGDSLEIDSLMRTSFFLKGSTNAALNTVFLV
                     DSKFHLRGIYDGADLLEMKRLKDDIKILSYETRQKSK"
     gene            complement(6570..6935)
                     /locus_tag="PKY09_10550"
     CDS             complement(6570..6935)
                     /locus_tag="PKY09_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNB13335.1"
                     /translation="MSHNAQNEHKPHGYYTGDIHNQFVEEHYDPTAKKIIWRTFWILL
                     VATIVEVAIAFIPMHKELLQWIFVALTIFKSAYIVGVFMHLGKEKKSLQYTILLPFFL
                     IVYMIAICLIEGVYLFHIG"
     gene            complement(6961..7776)
                     /locus_tag="PKY09_10555"
     CDS             complement(6961..7776)
                     /locus_tag="PKY09_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014033195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNB13336.1"
                     /translation="MSTATEAKSKSIWGGGISPFNVSWGKMFMWFFLVSDAFTFSTLL
                     VAYGTMRHRFTDIWPAPGDVFTHFPFIHGHVPLAYVGLMTFILIMSSVTMVLAVDAGH
                     QRNHKSVAMWMGLTIVGGLMFLGSQAWEWYHFIHGSKEGTYILADGRFARTDDYEPGK
                     LFFWHNHQKTVVEGAEAEKLKASAVRNIKGANMTENEYGNTPLFANFFFFITGFHGFH
                     VFSGVVINIIIFINVLNGVYKRRGHYEQVEKSGLYWHFVDLVWVFVFTFFYLV"
     gene            complement(7909..8484)
                     /locus_tag="PKY09_10560"
     CDS             complement(7909..8484)
                     /locus_tag="PKY09_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNB13337.1"
                     /translation="MQTATTEQDNNYTGIPTAKILLWIAMASMVMLFAGLTSAYIVRK
                     NEGNWIEFSLPKMFTISTIVIIISSFIIQYAVYAIKRNLLNQVKIAVITTLGLGIAFV
                     FMQFYAWTVMVENGIHLVGNPSGSFIYVLSGMHILHLAGGIISMIVISVKSIQEKYSP
                     KNYLGIQLGAIFWHFLDILWVYLFVFVTFIA"
     gene            complement(8490..9377)
                     /gene="cyoE"
                     /locus_tag="PKY09_10565"
     CDS             complement(8490..9377)
                     /gene="cyoE"
                     /locus_tag="PKY09_10565"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNB13338.1"
                     /translation="MLKTTMVVAPRTFTARLVDYGQLFKLRLASLVVLSAALAFLIGS
                     TTVDYTKLWLLVLGGFLVTGSSNGLNQIYERRTDKIMKRTRLRPLPDSRMSLTESLIV
                     TISAGVIGLFILTYYINLYSGILGFIALVSYAFAYTPLKGVTPLSVFVGAFPGAIPTL
                     LGWVAATGQFSYQGWILFAIQFMWQFPHFWAIAWKLYDDYMSAGFQLLPAGGKNSASA
                     FQTTIYTFTLIPIGLLPYYSGMSGVISAIVITLAGILFFMQSLYLLKTCSEDAAKKLM
                     FGSFIYLPVVLIAILLDKI"
     gene            complement(9525..9887)
                     /locus_tag="PKY09_10570"
     CDS             complement(9525..9887)
                     /locus_tag="PKY09_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13339.1"
                     /translation="MLNSTNNSVITGTKNSGGVCEDLNNLMINIFSATLRLRISVFEF
                     YYFCQKLLNMLNSFWHGIAAFFEFIFELAKPLGKSIDIIFMVLIAIGLLYWLIYEKRV
                     ISGKTRNYLADKVKEKSK"
     gene            9919..10788
                     /locus_tag="PKY09_10575"
     CDS             9919..10788
                     /locus_tag="PKY09_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13340.1"
                     /translation="MNHGVLYALLLDSKIDGIKWAVPALIFVLITIQAYLFNTLINRF
                     EVLYKNSNIPVIIFIILYGLIPDFLSLSPFILVNTLMPLALWKIFALYKSERPVTRAF
                     DTGFVFSLMILCYLPMALLVVFMYLCIAILRPFNWREWVASALGLATPAILLFTILLV
                     TNKMHLRITEVNTDTWLPVIKLQSLSWHYILPALVIAVILFLSFLKLYANYSKNVIRM
                     RKFQLCFMLLLVFLLLIILIPVQQSAARFSLLIPPICLFLSYYFIAIRKIWWFEILST
                     VFILLIALGYAIL"
     gene            11161..>12061
                     /locus_tag="PKY09_10580"
     CDS             11161..>12061
                     /locus_tag="PKY09_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNB13341.1"
                     /translation="MSAMLWTTGAQAQIAGYGFTQVAGSYSPVSGGTQLIAAAVDDGS
                     SAATNIGFTFNLAGTNYTQFVANSNGHIRLGATNPTSSYSPISTTSNTGAISGFGRDG
                     RTGGAVVYLLSGSAPNRVLTIDYPDFNATYSPATYKQSFQIKLFETTNVIQIIYGPNA
                     SPTTYTCQVGIRATAADFANRTTTTNWAASTAGTVSSTMTWSATVYPANGLTYTWTPP
                     ACAAPSALAATPTTSTTANISWTAPLTPPSNGYEYAVTTSAVPPPSGTAFAGTSTSVS
                     GLTANTTYYLHVRSYCGGSDYSAW"
BASE COUNT         3972 a         2190 c         2247 g         3652 t
ORIGIN      
        1 ttgttatttg aaaatgacgt taccggtgat gcagattata aaactactat tgttgatgtt
       61 tctgttgcct ggaattcttt tttagatgaa aacaaaaaac atcagctcac cttagccatt
      121 caaccgggat ggatgcagaa cggcttgaat ttttctgaac tgtatttcgg ttcacaatat
      181 gatggtgata tttttgatcc taatctgcct acaggtgaaa accccgaacg cagcagttat
      241 tccaatttta atcttaatgc aggtgccggt tatagatatg cttttagtga aaactctgat
      301 ttgaatttgg ttttcggtgc cagccacctg aatcagccgg atatttcgtt tctgaatgat
      361 aaagcaaaat tatttacgcg actcacaggt attggagttt taaacctgat gctttctgaa
      421 aaaatttctc tgcaaccaca agtgctattt tatcgtcagg gaaaattctc gcaattaaat
      481 ggaggtgcta atgttcagct acattcttta gagaataatt taaaaaattt cagattttca
      541 gcagggctgt tttacaggaa taaagatgcc gttattgccc gtattggtgc cggcattgga
      601 aatttagatg ccggttttgc atacgatttt aatacttctg atcttaaaag ggcaagtaac
      661 ggtagaggcg cttacgaagt tgcattgtcc tacatcatca ctaaagtaaa accattaaag
      721 aagaatccac catgcccggt ttattaacat ttgtttttgg cagcaggagg catgttgttg
      781 ccagggttgt aatgtttgcc tccttgtttt ttgctgcgca gcaattgcat gcgcaatctc
      841 cggcagctta tgaacgtgca ggtgacaagg ctatgaaaga gggcgattat tattcagcat
      901 tcactttcta ttttaattct tatgctaaca acaatacaga tttaattatt acctggaaat
      961 tggcagaatc atgcagattg tacaatgatt atgagaatgc acagaaatat tataacaaag
     1021 cttatggatt agataagcag ggtcagtttc cattaatcgc attttatctg gcagagatga
     1081 atcagcgcca gggatattat gaagaggctg cttcaatcta caataattat ttacaaatgt
     1141 atggacgcga caatagttat tttacagaca aagcaaaacg cgaattaaac aattgccaaa
     1201 acattaaaca gttgcttaag gacacagcta aggtttcaat tcaaaactca ggcactgaaa
     1261 taaattctgt ttattctgat tttgctccat catatttgga tagtacaacc atgttgtatt
     1321 catctcttcg atttgagaat aaagataaaa aaacgaagaa aaaaaataag tatatatcga
     1381 aattgcttat cagcaataat gaacgcaata cgtggagtag tcctgataat tacagtacac
     1441 agctcaatcg ccccggatta cattcatgta attctgcagt aagtggaaat ggaaaattgt
     1501 tgatatacac acaatgtcag cgtgaaaaag acaaattgat gtgcactttg tattacagca
     1561 cacagaaaga tggcaattgg actaatccac ttccactaaa cgacagcatt aattttaaag
     1621 gatatacagc tacaatgcct gctatcggaa atgatggact atcgggttat gtactttatt
     1681 tcgttagcga ccgtcccggt ggcttgggta aggcagacat ctggaagtgt tctattacgc
     1741 ctgattttat ttttggtaat cctgttaatg taggtgcacc ggttaatact gttgatgatg
     1801 acatcacacc attctatgac aatcagcatt caactttata ttttgcaagt gaagggcata
     1861 aagggcttgg aggttttgat atttacagtt ataaaacaga tgtcccggaa caaaacgtag
     1921 agaaccttca ctatccgatc aatacaagtt ataatgattt gtattatagc agtcgcaaca
     1981 ataatttttt actctcatca aaccgtcctg gctcactcta tataaaagca agaacctgct
     2041 gttatgatat ttatgaaggt gtatatttgg atacggttaa gcatgtcaag aaagatactt
     2101 taatggctat tgagcctttt gctaatgaca tttcacaaga ttcaatcact aaggaagtca
     2161 tcaggcaaat agaaccattg ttgccgatga agttatattt tgaaaacgat cagcccaatc
     2221 caaaaacaat gctgacaaca acaaaagcaa attatgttga cttgtaccat acatatctca
     2281 caaatgagaa aaagtacaga gtgaattatg ttgatgtttt aacagatgaa tcaaaacgca
     2341 caaatgctga tgctgaaatt acggccttct ttaatcaggt cgttaagaaa agctatcttg
     2401 accttgaccg tttcagcgac ttattattga agcaacttga tgcaggaaat aagattgaaa
     2461 taattgtgag aggatctgca agtccattgg cagcaagtgg ctacaacaag aatctgtcta
     2521 aacgaagaat cagcagtgta ataaatttct ggaaagttta taatgcaggc aagctaaata
     2581 attatatcaa gacaggaaaa cttaccatta ctgaagatgc cgtaggtgaa gaaatgacct
     2641 caacaaaaat tagtgacgac ctgaaagata aaagaaactc ggtttatagc ccggctgccg
     2701 gtagcatgcg ttacatacag gttatgaggg tgatgataaa cggtgtggct gtagaatagt
     2761 gatttttata actttatagt ggcaaacctg cctgtttatt cttacttttg ctcctgaaaa
     2821 cagaaaaaat tgaatagcga aatttcagaa aaagtaagcc gtttgcgtga gcaggccgat
     2881 gcactaatgt cggtaaatac tgctaaggca ataattcttc tcagcgaagc catgaatcag
     2941 attgaagctg atgatgaaaa actcaaatcg gaatgtggat tgcagttggc attggcttat
     3001 cacaaggtga aagaagagtt gatggccata aaaataatta accaatgtct tgagttaaaa
     3061 attaatagtg acagtttaaa gaatcagatt ccttttaatg agctggctgc agaaatctat
     3121 actgcaattt ctatttacga cagtgcactt gaacatttgc ttaatattgc ttcagcctac
     3181 acatcagcaa aggacaagca gaaactcggt ttggttttaa atcagattgg tgaaacacat
     3241 aaattactcg gtggttataa tgaggcaatt cgccatcatg aaaaagcatt aaaaattttt
     3301 gaggaacttg aaaataagga acagctttct ataaccaatt tctatatcgg taattgttat
     3361 aactgggctg atgaattgga tattgccttc aattatcttg atacgagttt gaaacttgct
     3421 gagaaacttc gcagtcctga attaaagata aagacattag gtagcttagc aatattattt
     3481 accaagcaga ataaattcga gaaatcactc gactattttt ttcaagctat tgataacagc
     3541 aacctcgcag gtaatgtacg tgtgaaggcc gacttattaa agagccttgg taatctttat
     3601 aatcaaatgc acaggcatga tgaggctata aaaattttac ttgaagcaga cgaaatctgt
     3661 caatctctta atattaaaca gccgctgcat ctggtaaatt tgtttttatc tgatgcctat
     3721 gctgcattaa ataatttcga aaaagcatta gcacatcata aagtatatta taaactttgt
     3781 cgcgaaatac aaagtgaaga tgtaagtcat agaactaaaa acttgcagct taaatatgat
     3841 ctggaagaaa tacaaagaga gaagaacaaa gcagaagaat cacttttact aaaagataca
     3901 tttcttgcaa atgttagcca tgagttgaga actccgctta atggaattat cggtatggcc
     3961 gatttattat ctgaaacagt agctaccgat gagaacagaa aatatgttca gactattctc
     4021 tcatcagcgt ttcaattatc aaatattgtt gacgaaatat tagactatgc gcgagtaaat
     4081 acaggtcagg tttgcccggt agagcatcct tttattttaa acgaaacgat aaaagagtgt
     4141 ctcgaaaaat ttcttttaaa attccccgga aagaaagttg aatccgatac cgattttaaa
     4201 gcttgtgatg aagtgttgct tgatgagggt attcttaaaa aagttgtttt taacacgctt
     4261 gtttttggtg gttcatttgc cagtaacgat aaacttgagc tggcattata tcattcacag
     4321 aacaaaatat ttatcaatgt aaaattcaac attgtaaata aaaagagttt tccgcatcct
     4381 gataacttct ttgagctcaa caatccgcag ttttctcaga atggattgaa tggatacgga
     4441 ttagggttgt cactgatcaa tgcacgaggt atgattatgg cttgtggagg aaaaatttca
     4501 gtgaagcttg aaagttcaaa atgcaacttt gttattgagc tgccttataa agctgttaag
     4561 tcagacagaa aaaatattgt aaatgaaccc accgctacag aagatgtaac aatattattg
     4621 gtagaagaca ataaaataaa tcagtttctt gccaaaacaa tgttgcagaa gaaaggctat
     4681 aaagtagcac tggcaaccga tggtgaagag gctttgaatc ttcttgaaaa aggtaacttc
     4741 agtatggtaa ttacagatgt tcagatgcct ggaatggatg gttaccaact ggccgggcat
     4801 atacgaagta aactatccgg tcctgttaat gcaatcccta ttcttgcatt gacggcttat
     4861 gagtcaagtg cagagaaaga gaaggcaaaa gctgctggta tgtccggctt tttaactaaa
     4921 ccctacatgc ctgaacaact ttttgacctc atagcgcaac atcttccatt gaaaaatgat
     4981 aaattgaatg atgcagctga atcggaatca tcttttaatg ttaatgaaat ttatgagaac
     5041 ctccttgctt taatggcagg tgataaaaaa gaaacagctt ccttgataaa gatgctgcaa
     5101 gtacaatttc ctgaattgct cagcgaaatg ttacaggcca tagaagacaa ggatatgaag
     5161 aaactgtata aggcagcgca taaaataaaa tcaagtctga aaatattcaa caacaattct
     5221 gtaggagtat taatagatca ggtagaacgc aaggccaaag acaatgaata ctccttcgag
     5281 ctatcagtaa aagtatcaga gcttattaaa caagtgaatg taattatagg ctcacttgac
     5341 caactggtaa aaaagtaaac agaaaatttt attggttaaa tgaatagtaa ggtgatatca
     5401 tcagatatac aatcacgcct gttaatgtaa catacagcca tataggaaat gaaaaccttg
     5461 tcagcttacg atgttttcgc acatccattt ttaggccata ataaaatgat agcagtacca
     5521 agggcattac tatggcagca agaataatat gagatgctaa aatgaataaa tacactccgc
     5581 gcagcggatg gtcaacagga tatttggttt cttcagctaa atagtgatat aaaaaatacg
     5641 aaacaataaa aacagcactc aataaaaaag taagcaggtt tattttttta tgaatgtcta
     5701 ttcgtttctt tttaataaaa taaaatgaaa tcagcagcag gataaaacag gttccgttta
     5761 ccaaagcatt taaaccgggc aggtacttcg taaattccgg aggcgtttcc gggcgtggaa
     5821 gcactttctg actcagcagc atcaccattc ctaatacaac tactgaaata attgctacta
     5881 ctctgaaagt aagtttctca ttcattaaaa tctgtttcat cttactttga tttttgtctt
     5941 gtttcgtaac taagaatttt tatatcgtct ttcaagcgtt tcatctccag caaatcggca
     6001 ccatcatata tgccacgaag atgaaatttg ctgtcaacca aaaatacagt gttgagggct
     6061 gcattggtac tgccttttaa gaaaaacgat gtgcgcatta aagaatcaat ttccaatgaa
     6121 tcgcctgtac ataactgcca ttgtggtgga tgaatactgt tcattttcat tacctgcttc
     6181 aataccggaa tgctgtcgtt ttttggatgt gtggttatcg aaagcagttt caccttaggt
     6241 gcagttgcaa aatctcttgc cacatcatac atattggtaa aaacttcctt gcacggttcc
     6301 tgacatgagg cattaaaaaa ctgaaacacc caataggagg ggtcggtgtt gctgagtgaa
     6361 acagtatttc cttctgtatt ttttagtgag aaatcagtta gggtatgata aattgtgtca
     6421 actccgtttg ttgcaagatg cttgggtcca taatattcaa gatatctgaa attatgcttc
     6481 ccttctttca tcagaataaa aaaaaataag ggcatcagaa gtattaaaaa aaccgctgct
     6541 gccctaaact ttttcaagat aaaaatggct tatcctatat ggaaaagata aacgccttca
     6601 ataagacaaa ttgctatcat gtaaacaatc agaaaaaaag gtaaaagaat ggtgtactgc
     6661 agactctttt tttcttttcc aaggtgcata aatacaccta caatatatgc tgatttaaag
     6721 attgttaaag caacaaatat ccactgcagc aattctttat gcataggaat aaaggctatt
     6781 gctacttcaa caattgtagc aacaagcaat atccagaatg tgcgccagat aatttttttc
     6841 gctgtaggat cgtaatgctc ttcaacaaat tgattgtgta tatcaccggt gtaataaccg
     6901 tgtggtttat gctcattttg tgcgttatga ctcatgattc tatatttctt ttagaattag
     6961 ttaaacaaga tagaagaagg tgaacacgaa tacccaaacc aaatctacaa agtgccagta
     7021 gagccccgac ttttccacct gctcataatg accgcgcctt ttataaacgc cattcagcac
     7081 attaataaaa attataatgt tgatgactac accgctgaaa acgtgaaatc cgtgaaatcc
     7141 tgttataaag aaaaagaagt tagcaaataa tggggtattg ccatattcat tctcagtcat
     7201 gtttgctcct tttatgttgc gcactgcaga tgcttttagt ttttctgctt cagcaccttc
     7261 aacaacagtt ttttgatggt tatgccagaa aaataatttt ccgggttcat aatcgtctgt
     7321 acgcgcaaaa cgtccatcag caagaatata agttccttct ttagaaccat gaataaagtg
     7381 ataccactcc catgcctgac tgcctaagaa catcagaccc ccgacaatgg ttaatcccat
     7441 ccacatggca acacttttat ggttacgctg atgtccggca tctactgcaa gtaccattgt
     7501 tacagaactc atgatgagga taaaggtcat taatcctaca taagctaaag gtacatgccc
     7561 gtgaataaaa gggaagtgcg taaacacgtc acccggtgca ggccatatat ctgtaaaacg
     7621 atggcgcatg gtaccataag ccaccaggag agttgagaat gtaaacgcat cagataccag
     7681 gaaaaaccac ataaacattt ttccccagct tacattgaat ggtgatatac caccacccca
     7741 gatggatttt gattttgctt cagttgctgt tgacatatta agttaggagt cttgaaatta
     7801 ttttacagga tgcaaatatt taattttttt tccaattcat caatttattg taaaattttc
     7861 ggctatttaa ccataattca ttgacagatt ggagtaaaaa atgacccatc aggctatgaa
     7921 ggtcacaaat acaaagagat aaacccacag gatatcaaga aaatgccaga aaatagcgcc
     7981 taactgaatt cctaaatagt ttttgggaga atacttttct tgaattgatt ttacagatat
     8041 cacaatcatt gaaataattc ctcctgccag gtgaaggatg tgcataccgg ataatacata
     8101 gataaaagaa cctgatggat tacctactaa atggataccg ttttcaacca taactgtcca
     8161 ggcataaaat tgcataaaca caaaagctat acccagtcca agagtggtta ttactgcaat
     8221 tttaacctga ttcaacagat ttcttttaat ggcataaacg gcatattgaa taataaaact
     8281 gctgataata ataacaatgg tgctgatggt aaacatcttt ggtaagctga actcaatcca
     8341 gttaccttcg tttttacgaa caatataagc acttgtcaat cctgcaaata gcataaccat
     8401 actggccatg gctatccaca ataatatttt tgctgtagga atgcctgtgt agttgttatc
     8461 ttgttctgtg gtggcagttt gcattgtaat taaattttat caagtaatat ggcaattaat
     8521 actacgggga gatatataaa agacccaaac atcagctttt tggctgcatc ttcggaacat
     8581 gttttaagta aataaagact ttgcataaag aaaagaattc ctgcaagggt aatgactatt
     8641 gcagaaataa cgccactcat tccgctgtaa tagggcagca gaccaatggg tataagcgtg
     8701 aaagtataaa ttgttgtttg aaatgcagaa gcggaatttt taccacctgc aggaagcaat
     8761 tgaaagccgg cactcatata gtcgtcatag agtttccagg caatagccca aaagtgtgga
     8821 aactgccaca taaactgaat ggcaaaaagt atccatccct gataactaaa ctgaccggtg
     8881 gcagcaaccc agccaaggag ggtagggata gctcccggaa atgctccaac aaaaaccgat
     8941 aaaggggtaa caccttttaa gggtgtgtag gcaaaggcat aacttaccaa tgctataaaa
     9001 ccaagtattc cactgtaaag attaatgtag taggtgagta taaacaatcc tattacaccg
     9061 gcagagatcg ttacaataag cgattctgta agcgacattc tgctgtcagg cagcgggcgt
     9121 agccttgtac gcttcattat tttatcggtg cgtctttcgt aaatctgatt caatccgttt
     9181 gatgaacctg taactaaaaa gccacccaaa acaagcagcc acagctttgt ataatctacg
     9241 gttgttgagc ctataagaaa agctaatgca gcagagagaa ctactaacga agccagcctt
     9301 agtttaaaca actgtccata atcaacaagt ctggcagtaa aggtacgtgg tgcaacaacc
     9361 attgttgtct tcaacattta agcaatctga aaaaatttcg gaaggcaaag gtataaaatt
     9421 gataggaagg attattaaaa atgataatta cttattgata agcaaaaaaa aaggcagcct
     9481 ttggctgcct caaatattat tttaaagcta tgaaacagta gcgattactt actcttttct
     9541 tttactttat ctgcaagata gttgcgggtt tttccgctta ttaccctttt ttcgtagatg
     9601 agccaataca ataagccaat ggcaattaaa accatgaaaa taatatctat tgacttacct
     9661 aatggttttg ccagttcgaa aataaattca aaaaatgctg ctataccgtg ccagaatgag
     9721 tttaacatat tcaataattt ttggcaaaaa taataaaatt caaatacaga tattcgcagc
     9781 ctcaaagttg ctgaaaaaat gttgatcatc aggttgttta aatcttcgca aacgccacca
     9841 ctgtttttag tgccggtaat aacgctgttg ttggtgctgt tcaacattta tacccaaggg
     9901 gttgtcatta tcaaagacat gaatcatggt gtattatatg ccttattact cgacagtaaa
     9961 attgacggta taaaatgggc agtgcctgca ttgatttttg tactcatcac cattcaggcc
    10021 tatttattta atacactcat caaccggttt gaggttttat acaaaaacag caacattccg
    10081 gtcattattt ttatcatcct ctatggactt attcccgatt ttttatccct tagtcctttt
    10141 attctggtaa atacattaat gccgcttgcc ttatggaaaa tatttgcatt gtataaatca
    10201 gaacgtcctg ttacccgtgc ttttgataca ggctttgttt tttcgctgat gatattgtgt
    10261 tatctgccca tggcattgtt ggtggttttt atgtacttgt gtattgccat attacggcct
    10321 ttcaactggc gcgagtgggt tgcttctgca ttaggacttg caacaccagc tatacttttg
    10381 tttaccatcc ttttggtaac aaacaaaatg cacctgcgaa ttacagaagt aaatacagat
    10441 acatggctac ctgttatcaa actacaatca ctttcatggc attacatatt gcctgcattg
    10501 gtaatagcag taattttatt tttatccttt ctgaaacttt atgccaatta cagtaagaat
    10561 gttatccgca tgcgaaaatt tcagttatgc tttatgttat tacttgtttt tctgttactg
    10621 ataatattaa ttccagtgca acaaagtgct gcaaggttta gccttcttat ccctccgata
    10681 tgcctgtttt taagctacta ttttattgca attcgcaaga tatggtggtt cgaaatcctt
    10741 tcaacggttt ttattttact aattgcctta ggatacgcta ttctttaagc ttttgatttt
    10801 tattaaggaa tgataataat cagtttttgg gttttatctt tttaaaaata attaatttat
    10861 tgataatgat gtgataatgt ttttggtttg tcataaaagt ttcaaaatac ttttttgtgc
    10921 ttgtttaatt aatatcaaat gaataatatt gaggccgatt tctatttccg attttgcatt
    10981 tagatgctat tctacaagat cccaaaggtc ttttttaatt gcgctttaca acttcggttt
    11041 aatttaatgt agtcgcaaag tattttaatc aattaatgtt taactaaaat caaatttaac
    11101 atggtagaaa aaaccaaaaa tcgaacacga acgtgtttat tctttttatg gtgttgcctc
    11161 atgtcggcaa tgctatggac aacgggcgca caggcgcaaa tagcaggtta cggcttcact
    11221 caggtagccg gatcttactc acccgtaagt ggtggcacac aactgattgc tgctgctgta
    11281 gatgatggaa gctcagcggc aaccaacatt ggctttacct ttaatctggc aggcaccaac
    11341 tacacacagt ttgttgctaa ttctaacgga cacatccgtt taggagcgac caatccaact
    11401 tctagttatt cacctatctc tacaacctcc aacaccggag ctatttccgg ctttggcaga
    11461 gatggaagga ctggtggtgc tgttgtttac ttactttccg gatcagcacc aaacagggta
    11521 ctcaccattg attatcctga ttttaatgca acttactccc ctgcaaccta caagcaaagt
    11581 tttcagatta aattatttga aactaccaat gttattcaaa taatttatgg gcctaatgca
    11641 tcacctacaa cttatacttg tcaggtaggt atcagagcca ccgctgctga ttttgcaaac
    11701 agaacaacta ctactaactg ggctgcctca acagcaggta cagtttcttc aaccatgacc
    11761 tggagtgcaa cagtgtatcc ggctaatggc ttaacctaca cctggacacc accggcatgt
    11821 gcagcaccaa gtgctttagc tgcaacacca acaacatcaa ccactgccaa catcagctgg
    11881 acagcacctt taacaccacc atcaaatggt tatgaatatg cagtaactac aagtgctgtg
    11941 cctcctccat caggaactgc ctttgcaggc acatcaacca gtgtgagtgg cttaactgct
    12001 aatactactt actacctgca tgtaagaagc tactgtggtg gcagtgatta tagtgcatgg
    12061 g
//
LOCUS       DAOQNP010000084        12003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1885_length_12003_cov_9.195932, whole genome shotgun sequence.
ACCESSION   DAOQNP010000084 DAOQNP010000000
VERSION     DAOQNP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1885_length_12003_cov_9.195932"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /gene="metG"
                     /locus_tag="PKY09_10585"
     CDS             complement(<1..2038)
                     /gene="metG"
                     /locus_tag="PKY09_10585"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNB13342.1"
                     /translation="MSRYLITAALPYANGPVHIGHLAGCYIPADIYSRYLRLKGEDVK
                     FICGSDEHGVPITIKARQLGITPQQVVDKYHQMMKSSFEKFGISFDHYSRTSSPVHHQ
                     TAAAFFKTLYDKDVFIEETTEQFYDEEAKQFLADRYITGTCPRCNNPNAYGDQCENCG
                     ASLSPTDLINPKSKLSGNTPVLRPTKNWFLPMGEIQKSNDFQEYIKRFDHWKPNIKGQ
                     CNSWLSEGLQPRAMTRDLDWGVKVPLPHTEGKVLYVWFDAPIGYISATKELLPDNWKD
                     YWHKQNNEEDNPKLIHFIGKDNIVFHAIIFPMMLMQHGGFVLPYNVPANEFLNLEGDK
                     ISTSRNWAVWLHEYLEDFPGREDELRYVLTSIAPETKDSEFTWKDFQARVNNELGAIL
                     GNFVNRVVVLSNKYFKGNMTTDKKISVAPFDNELNKLLLSYTKAMKNFQFRDAQAIAM
                     DFARIGNKYLTACEPWKKYKAEDVDSVNQTREVLIHCLSLISKMAVCFQPFLPNASAK
                     ILDILKVDSAKRKLAYNGFENSIISLNAIVLNINSLILFQKIEDTDIEKQIQKLHDKK
                     VMNTEQNQQAVVVGETTIEPQLDETTFDDFSKMDIRVATILEAERVPKTDKLLKLLLD
                     TGIDKRTVVSGIAAWYDPEKIIGQKVVMLANLAPRKIKGIESKGMILMAQNNKGE"
     gene            2173..3084
                     /locus_tag="PKY09_10590"
     CDS             2173..3084
                     /locus_tag="PKY09_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNB13343.1"
                     /translation="MIFPPPLVAGDTVAIVATARKVSRDEMAPAIGILQQWGLQVAEG
                     KNLYADKNQFAGDDSLRAEDFQWALDNPKIKAVLFARGGYGSVRIIDRINFSEFKINP
                     KWLIGFSDITTIHSHVHNQLNVATLHAPMAINFPKTPDAVLKQIKDFLFGNSIVLNAQ
                     AHLLNRMGNAKGQLVGGNLSILYSLTASASDIDTKGKILFLEDLDEYLYHIDRMMMQL
                     KRCGKLHSLAGLVVGAMSDMKDNTIPFGKTAEEIIAEHVSEYDFPVCFNFPAGHITEN
                     NPLALGGYAKLIVANKVSLKVNNKALS"
     gene            complement(3092..3448)
                     /locus_tag="PKY09_10595"
     CDS             complement(3092..3448)
                     /locus_tag="PKY09_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNB13344.1"
                     /translation="MSVLPPCPLCHSIYTYENGNLMVCPECFYEWNPEEQQAVSEETS
                     SADNGKVFDANGNELQNGDAVIVIKDLPVKGASKPVKAGTKVKNIRLAEGDHNIDCKI
                     EGFGSMALKSEFVRRA"
     gene            3589..4533
                     /locus_tag="PKY09_10600"
     CDS             3589..4533
                     /locus_tag="PKY09_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNB13345.1"
                     /translation="MKKKPPFHNLRSIFKQYNTFTYSERKGIIVLSIIIVALLLTLYG
                     LKFYEPPITINTGRLDSAVAQWNLLQADSIIDVKDSTKLSESNATQPTRSLFSFNPNN
                     LADSLWAKLGLSEKQIASIKKYEDRGGRFRIRSDVKKLFVITPSFYDSIYPYLLLPDT
                     LPAFHQTKFAADSIQSKKEKVIEPIEINLADVEEWQLLPAIGAGRAAAIVAYRSKLGG
                     FTSVKQLLEINSIVDSVFEKISPYVLCNIKPIQKKNINTDDAYSLRHPYISSSLATII
                     VNYRKMHGDYKSTEQLRDLKIIKPTDFEKLAPYIVVGF"
     gene            4660..5229
                     /locus_tag="PKY09_10605"
     CDS             4660..5229
                     /locus_tag="PKY09_10605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNB13346.1"
                     /translation="MDYFKHESSYVDDGCSIGKGTKIWHFSHIMSGCRIGEGCNIGQN
                     VVISPDVVLGNNVKVQNNVSLYTGVVCEDDVFLGPSMVFTNVTNPRSAVNRKNEYKKT
                     VVKKGASIGANATIVCGHDIGRFAFIGAGAVVTKDVADYALMAGNPARQMGWMSEYGH
                     RLKFDKDGMAVCPESGEKYLLQNNQVTKI"
     gene            5245..6300
                     /locus_tag="PKY09_10610"
     CDS             5245..6300
                     /locus_tag="PKY09_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNB13347.1"
                     /translation="MSSEKKEKIKFAVIGCGHIGKRHAEMVRRNNECELVAMCDIRQE
                     NEVGIADFNVPFFQSVDAMLTSIQDIDVVCVCSPNGVHAEHSLKALEAKKHVVCEKPM
                     ALSKADCEKIIFKALQVSRHVFCVMQNRYSPPSVWLKEIIENNIIGEIFMVQLQCYWN
                     RDERYYKKGGWKGTSDMDGGTLFTQFSHFLDIMYWVFGDITDIQGKFEDFTHQQNTSF
                     EDSGFVNFKFVNGGMGNIAYTTSVWDVNLESSITVIGKNGSIKIGGQYMNEVEYCHIK
                     DYTMPKLDPVNPANDYGAYKGSAANHHYIIENVADVLRGRTSITTNALEGLKVVEIIE
                     RIYALRKIEKSEIKFHL"
     gene            6306..7595
                     /locus_tag="PKY09_10615"
     CDS             6306..7595
                     /locus_tag="PKY09_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNB13348.1"
                     /translation="MYQEILDKKAKVAVIGLGYVGLPIALEFAKKISVIGFDINAERV
                     EKMQNKIDPSGELPAEAFDGCDIKFTNSLDVLREARFFIVAVPTPIDDHKLPDLKPLI
                     GASTSIGKVLKKGDYVVYESTVYPGCTEEDCIPVLEEHSGLKFPNDFKVGYSPERINP
                     GDKEHTITKIKKVVSGCDAESLDIIAKVYETIVTAGVHRASSIKVAEAAKIIENTQRD
                     VNIALMNELSIIFNKMGINTYEVIEASSTKWNFLRFFPGLVGGHCIGVDPYYLTYKAQ
                     ELGYHSQVINSGRYVNDSMGFYVAKQTVKKMIAKGKDISKSKVLVMGATFKEDVEDIR
                     NSKVTDVVNEFKSFGVTIEVTDPYASSDELMHEYGFGLVEKIANDYDAVVVAVSHKDY
                     LKMDENYFKSICKPDGVIVDIKGFLRNKIKNLQYWSL"
     gene            7660..8685
                     /gene="galE"
                     /locus_tag="PKY09_10620"
     CDS             7660..8685
                     /gene="galE"
                     /locus_tag="PKY09_10620"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009421421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNB13349.1"
                     /translation="MKILVTGGTGYIGAHTVVELMNSGFDVDIIDNLNNSKEEVVDAI
                     AKITGKRPLWFNIDLCDANEIKNYFSKHKPDAVVHFAAHKAVGLSVEQPLAFYHNNLL
                     SLINLLQCCVEAKCNSFVFSSSCSVYADPDKLPIDENAPVKTAKSPYGNTKKIGEEII
                     TDTVRSSSLQAVSLRYFNPVGSHDSALIGELPTTMPLNVMPIITQVAIGKRKQFVVFG
                     NDYPTPDGSCIRDYIHVVDVAKAHVTAVMRLLNKKNKSAYEVFNLGTGKGFSVLELIH
                     AFEKTTGQKLNYTIEGRRAGDVTAVYADTALANTVLGWHTERDLNDMIRTAWNWELYL
                     AGRTDFI"
     gene            8730..9797
                     /gene="rfbB"
                     /locus_tag="PKY09_10625"
     CDS             8730..9797
                     /gene="rfbB"
                     /locus_tag="PKY09_10625"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008244826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNB13350.1"
                     /translation="MSKSTIIITGGAGFIGSHVVRRFVNNYPDYKIYNFDNLTYAGNL
                     ANLKDIELKSNYHFIKGDITDAAFVNKLFADINPDAVIHLAAESHVDRSITNPLEFIM
                     TNIVGTVNLLNAARHTWIKDKKVSSGKRFYHISTDEVYGSLGNTGFFTENTSYDPRSP
                     YSASKASSDHLVMAYHHTYHLPVVLSNCSNNYGSHHFPEKLIPLAINNIKNMKPVPVY
                     GKGENVRDWLYVEDHARAIDVIFHKGKEGEKYNVGGNNEWKNIDLIRLLCSVMDKKLN
                     RKEGTSASLITFVTDRPGHDLRYAIDSTKLQNELGWKPSLQFEEGLEKTVDWYLANDA
                     WLQQVTSGEYMTYYQHQYDAR"
     gene            9807..10808
                     /locus_tag="PKY09_10630"
     CDS             9807..10808
                     /locus_tag="PKY09_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB13351.1"
                     /translation="MKETIYERAYHQQSLKDKAFLITGGSGFIGSNIVEYLLVHGAGK
                     VRVLDNLANGYKKNIEPFLKHPSFEFIEGDITNADTCLKAMKGIQYVSHQAALGSVPR
                     SLANPLATHHANATGFLNMLWAAKESGIERIVYASSSSVYGDSPELPKREHITGNPLS
                     PYAVSKMSNELYAYTFCMHYDMKIIGLRYFNVFGPNQNPDNPYAAAIPLFMKALLKDQ
                     PAYIFGDGEQSRDFTFVENAVQANIRALFSEHKDACNKVYNIAVGESISLNKLLKHLM
                     HITGKKIEPVFKEERPGDVKNSLADISLAMQYLGYNPLVKVKEGLALTVPWFKKNFC"
     gene            11320..>12003
                     /locus_tag="PKY09_10635"
     CDS             11320..>12003
                     /locus_tag="PKY09_10635"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNB13352.1"
                     /translation="MKSYTNTELKDIFKETEKTFFSQSTFNKSQLTEALEPFKQIKKV
                     KRIDNDYFWVMVYVAFYSGFKATTVTNKIGAIQDYFGDLDKVSKYDEAKVQKIIDSNK
                     VINHRQKINGIIHNAKQIKAIQKEFGSFDKYLESFGDTDQDDNFFDLVKDLKKRFKYL
                     GGITVFHFLTDIGFNVVKPDRVLCRIFNRLGLVDSEEDYAGVIKAGRQFSIANKVPIR
                     YIDIIFVVYG"
BASE COUNT         3809 a         2193 c         2336 g         3665 t
ORIGIN      
        1 gctctccttt gttattctgt gccatcaata tcattccttt gctttctata cctttaattt
       61 tacgtggagc aaggttggct aacatcacta ctttttgtcc gataattttt tcaggatcgt
      121 accatgctgc aatacctgaa acaaccgttc gtttatctat acctgtatcg agtagcaatt
      181 ttaaaagttt atcggtcttt ggtacacgct ctgcttctaa aattgtggca acacgaatat
      241 ccatcttgct gaaatcgtca aaagttgttt cgtcaagttg aggttcaatg gttgtttcgc
      301 caactacaac tgcttgctga ttctgttctg tgttcatcac ttttttatca tgaagtttct
      361 gaatttgttt ttcaatatca gtgtcttcta ttttttgaaa taagataagg ctgttaatat
      421 ttagaacaat agcatttaac gaaatgatac tgttttcaaa accattatag gcaagttttc
      481 tttttgcaga atctacctta agaatgtcaa gtatttttgc tgatgcattt ggcaaaaaag
      541 gttggaaaca cacagccatt tttgatatca atgaaaggca atgaatcagt acctctctgg
      601 tttgattaac gctatcaaca tcttctgctt tgtatttttt ccatggttca caagctgtca
      661 agtacttatt acctattcgt gcaaaatcca ttgctattgc ctgtgcatct ctgaactgaa
      721 aatttttcat tgctttggtg taagacaaca ataatttatt caattcattg tcgaaaggtg
      781 caactgaaat ttttttatct gttgtcatgt tgcctttaaa atacttatta ctcaacacca
      841 caaccctgtt cacaaagtta cctaaaattg cccccaactc attgttcact cttgcctgaa
      901 aatctttcca tgtaaattca ctgtccttgg tttcgggtgc aatagaagta agcacatagc
      961 gcagttcatc ttcgcgaccc ggaaaatctt caaggtattc atgcagccac acagcccaat
     1021 tgcgcgaggt tgatatttta tctccttcta gatttaaaaa ttcgttggca ggaacattgt
     1081 agggcaacac aaaaccgcca tgctgcatga gcatcatcgg aaaaatgatg gcatgaaaaa
     1141 caatattgtc tttgccgata aaatgaataa gcttaggatt gtcttcttca ttgttttgct
     1201 tatgccaata atctttccaa ttgtccggca aaagttcttt tgtagccgaa atatacccga
     1261 taggtgcatc aaaccaaaca tatagcactt tcccttctgt atggggtaaa ggcactttca
     1321 caccccagtc gaggtcgcgg gtcatggcac gaggctgcag gccttcgctg agccaactat
     1381 tgcactgtcc tttaatgttg ggtttccaat ggtcgaatcg tttaatatac tcctgaaaat
     1441 cgtttgactt ttgaatttca cccattggca aaaaccagtt ttttgttggt ctcagcactg
     1501 gggtattgcc actaagtttt gatttaggat ttatgaggtc tgttgggctg agtgatgcac
     1561 cgcagttttc acattggtcg ccataagcat ttggattgtt gcagcgcgga caggtgccgg
     1621 tgatatagcg gtcggcaaga aactgtttgg cttcctcatc ataaaactgc tctgtggttt
     1681 cttcaataaa aacatcttta tcataaagcg ttttaaagaa tgctgctgct gtctgatggt
     1741 gcaccggtga agaagtgcgt gaataatgat cgaaagaaat accgaatttt tcaaatgatg
     1801 atttcatcat ctgatggtat ttgtcaacca cttgctgcgg tgtaatacca agttgacgtg
     1861 ctttgatggt aataggtacg ccatgttcat cgctgccgca aataaatttt acatcttcgc
     1921 ctttcagccg cagataacga ctgtatatat cggcaggaat atagcaacct gccagatgac
     1981 ctatatgtac cggtccgttg gcatagggca gtgcagcggt gattaaatat cttgacatga
     2041 aaaatgttta acagttgtaa tgtataaaag ttatgaagaa tatattctga aaacgtttga
     2101 aaaaaaacat ttcgtactca ggattaaacc tcaaactgct tttactttgc aaagataaaa
     2161 aaacgaaggc agatgatttt tccacctcca cttgttgctg gcgataccgt tgccattgta
     2221 gctactgcac gcaaggtaag ccgcgatgaa atggctccgg ccattggcat actgcagcag
     2281 tgggggcttc aggttgctga gggcaaaaat ttatatgctg ataaaaatca gtttgccggt
     2341 gatgactctt tgcgtgcaga agattttcag tgggccctgg ataatccaaa aataaaagct
     2401 gtcctttttg cccgtggagg ttatggttct gtaagaatta ttgacagaat taatttctcg
     2461 gaattcaaaa taaatcccaa gtggctgata ggcttcagcg acatcactac cattcactca
     2521 catgtacaca atcagttgaa cgtagctaca ctgcatgcac ccatggcaat aaattttcct
     2581 aaaacacctg atgctgtttt aaagcagatt aaagattttc tttttggcaa cagcatcgtg
     2641 ttaaatgctc aagcacatct tttaaacaga atgggaaatg ctaaggggca attggttggc
     2701 ggcaatcttt ctattttgta ttcactaact gcatcagctt ctgacattga caccaaagga
     2761 aaaattcttt ttttggaaga tttggatgaa tatctctatc acattgaccg tatgatgatg
     2821 caactcaaac gctgtggaaa gttacatagt ttggcaggac ttgttgttgg tgcaatgagc
     2881 gatatgaaag acaacactat tccatttgga aaaactgctg aagaaattat tgccgaacat
     2941 gtttctgaat atgattttcc tgtatgtttt aattttcctg cagggcatat tacagagaat
     3001 aacccgctgg cattgggagg ctatgcaaaa cttattgtag caaataaggt gtcactaaaa
     3061 gtgaataata aagcgctaag ctgattttat cctaagccct tcttacaaat tccgatttga
     3121 gtgccataga gccaaagcct tctattttgc aatcaatgtt gtgatcgcct tctgctaaac
     3181 gtatgttctt aaccttggtg ccggctttca caggtttaga tgctcccttt actggaagat
     3241 cttttatgac aattactgca tcgccatttt gcaattcatt tccgttagca tcaaaaactt
     3301 ttccgttatc tgcacttgaa gtttcttctg aaacagcttg ttgttcctca ggattccact
     3361 cgtaaaaaca ttccggacaa accatcagat taccattttc ataggtataa atagaatggc
     3421 ataaagggca gggcggcaaa acagacatgg taaaaatatt ttctgcaaat atctttaaaa
     3481 ttcaccaaat tatactatca gataattaaa gaatattttt tcacttgcat acaaacagca
     3541 tttaaaacca ctattttcgc tttagaaaat taacttaccg ttttaaccgt gaaaaagaaa
     3601 ccgccttttc ataacctacg gagtattttt aaacagtaca acacgtttac ttacagtgag
     3661 cgtaaaggca ttattgtttt gtccataatt attgttgcac tgctgcttac actttatgga
     3721 cttaaatttt atgagccacc aatcactatc aatacaggtc gtttagactc agctgttgca
     3781 caatggaatt tgttacaggc cgattctatt attgatgtca aagactcaac aaaactttca
     3841 gaaagtaatg ctacacaacc tacaagaagt cttttctcat tcaacccaaa taatttagct
     3901 gattctcttt gggccaagtt aggtttgtct gaaaaacaaa ttgcatcaat caaaaaatat
     3961 gaagatcgtg gaggaagatt cagaattcgg tccgatgtaa aaaaactgtt tgtcatcacg
     4021 ccttcgtttt acgacagcat ttatccttat ctgcttttac ctgatacttt accggcattc
     4081 catcaaacaa aatttgcggc tgactctata cagtctaaaa aagaaaaggt cattgaacct
     4141 attgaaatta atcttgcaga tgttgaagag tggcagctgt tgccggccat aggtgcagga
     4201 agagctgcag caattgttgc ctatcgtagt aaattaggtg gttttacatc tgttaaacag
     4261 ttgttggaaa tcaattctat tgtggattct gtttttgaaa aaatcagtcc ttatgtactt
     4321 tgtaatataa agcccattca aaagaaaaac atcaataccg atgatgccta cagcctcaga
     4381 catccatata tcagcagcag tctggcaact ataattgtaa actacagaaa aatgcatggc
     4441 gactataaat caaccgaaca attacgcgat ttgaaaataa ttaaaccgac agattttgaa
     4501 aaacttgcac cctatatagt tgtagggttt tagcagcaaa aactcaggcc gaactcaaaa
     4561 atgtaagatt atgaccatct taatctgaaa acagactatc attcttatca aaataaaagt
     4621 aaatttgcac cccaattaca gcgggttaag gaagcgatta tggattattt taaacatgaa
     4681 tcatcctatg ttgacgatgg atgttccata ggaaagggta ccaagatatg gcacttttca
     4741 catattatgt ccggttgtcg cattggcgaa ggttgtaata ttggtcaaaa tgtggttatc
     4801 tcacctgatg ttgttttggg taacaatgta aaagttcaga ataatgtttc gttatataca
     4861 ggcgttgttt gcgaagacga tgttttttta ggaccgagca tggtctttac caatgtaacc
     4921 aacccacgta gtgccgtaaa ccgcaagaat gagtataaaa aaactgttgt aaaaaaaggt
     4981 gcttccatag gtgctaatgc cactattgtt tgtgggcatg atataggtcg gtttgccttt
     5041 attggtgccg gagcagtggt aacaaaagat gttgctgact atgcattgat ggctggtaat
     5101 cctgcccgtc aaatgggatg gatgagtgaa tatggtcatc gcctgaaatt tgataaagat
     5161 ggaatggcag tgtgccctga aagcggagaa aaatacctgc tgcaaaataa tcaggtaaca
     5221 aagatttaga aagaccgcac ttgtatgagc tcagaaaaaa aagaaaaaat aaaatttgct
     5281 gtaatcggtt gcggacacat tggtaagcgt catgccgaaa tggttagacg caacaacgaa
     5341 tgtgagcttg ttgccatgtg cgacatcaga caggaaaacg aagtaggcat tgccgacttt
     5401 aatgttccat tttttcagtc tgttgatgcc atgctcacca gcattcagga tattgatgtg
     5461 gtgtgtgttt gctcacccaa tggcgttcat gccgaacatt ctctgaaagc attagaagca
     5521 aaaaaacatg tggtttgcga aaaaccaatg gcactgtcaa aagccgattg cgaaaaaatt
     5581 atttttaaag cacttcaggt ttctcggcat gtgttttgtg taatgcaaaa ccgctattca
     5641 ccaccttcgg tttggcttaa agaaattatt gaaaacaata tcattggcga aatttttatg
     5701 gtacagttgc aatgctattg gaaccgtgat gaacgttact ataaaaaagg cggatggaaa
     5761 ggcaccagcg acatggatgg tggaacgttg tttacacagt tctcgcactt tttagatatt
     5821 atgtattggg tgtttggtga tataacagat atacaaggta agtttgaaga ttttacacat
     5881 cagcaaaaca catcatttga agacagtggg tttgtaaact ttaagttcgt aaacggaggc
     5941 atgggcaaca ttgcctacac tacctctgtt tgggatgtaa accttgaaag cagtattacc
     6001 gtaattggta aaaacggctc tataaaaatt ggcggtcagt atatgaatga agtagaatac
     6061 tgtcatatca aagattatac aatgcctaaa cttgaccccg ttaatccggc aaacgattat
     6121 ggagcttaca aaggctctgc tgccaaccat cactacatca ttgaaaatgt ggcagatgtt
     6181 ttgcgtggtc gcacttcaat aactaccaat gcccttgaag gactaaaagt tgtagaaatc
     6241 attgagcgta tttacgcact tcgtaaaata gaaaaatcag aaataaaatt tcatctttaa
     6301 aataaatgta tcaggagata ttagataaaa aagcaaaagt tgctgttatt ggtttaggtt
     6361 atgtcggact tcctattgca ctggaatttg caaagaagat ttccgtaatc ggattcgaca
     6421 ttaatgcaga acgcgttgaa aaaatgcaaa acaaaattga ccccagcggt gaattacctg
     6481 ctgaggcttt tgatggttgt gatattaaat tcactaactc attggatgta cttcgtgaag
     6541 cacgtttttt tattgtagct gttcctactc cgattgacga tcataaacta cctgacctga
     6601 agcctttaat tggtgcttca acctctattg gcaaagtgct gaaaaaaggt gattatgttg
     6661 tgtatgaaag taccgtttat ccgggttgta cagaagaaga ttgtattcct gtacttgaag
     6721 aacattcagg tttaaaattt ccaaacgatt ttaaggtagg ctactcacct gaacgtatca
     6781 accccggtga taaagaacat accatcacaa aaatcaaaaa agtagtttca ggttgcgatg
     6841 ctgaatcgct tgatattatt gccaaggttt atgaaacaat tgtaacagca ggtgtgcatc
     6901 gtgccagcag cattaaagtt gctgaagctg caaaaattat tgaaaacaca cagcgtgatg
     6961 tcaacattgc attgatgaat gagctttcta tcatcttcaa taaaatgggc ataaacacct
     7021 atgaggttat tgaggcaagc agtacaaagt ggaacttcct tcgctttttt ccgggcttgg
     7081 taggcggaca ttgtataggc gtagatcctt actaccttac ctacaaagcg caggagcttg
     7141 gctaccactc gcaagtaatt aactcaggca gatacgttaa cgacagcatg ggattttatg
     7201 ttgccaaaca aaccgtaaag aaaatgattg ccaaaggcaa ggatatttct aaatcgaaag
     7261 tgctggtgat gggtgctacg tttaaagaag atgtagaaga tatccgcaac agtaaggtta
     7321 ctgatgtggt gaacgaattt aaatcatttg gtgtaaccat agaagttacc gatccttatg
     7381 cttcttctga tgagttgatg catgaatatg gttttggttt ggttgaaaaa attgctaatg
     7441 attatgatgc tgtggtggta gcggtttcgc ataaagatta tcttaaaatg gatgaaaatt
     7501 attttaagag catctgtaag cccgatggcg ttattgttga cataaaagga tttttaagaa
     7561 ataaaatcaa gaatcttcaa tactggagtt tgtaaatata atacggcaac accctgaccg
     7621 ttatatagag atattaatcc ggtaaattta ttgcatgtaa tgaaaatatt ggtcacagga
     7681 ggcaccggtt atattggtgc acacactgtt gtagaactaa tgaacagtgg ttttgatgtt
     7741 gatattattg acaaccttaa taattcaaaa gaagaagttg ttgatgctat tgcaaaaatt
     7801 accggcaaac gcccattatg gtttaacatt gacctttgtg atgctaatga aataaaaaac
     7861 tatttcagca aacataaacc tgatgcagtt gtgcattttg cagcacacaa agcagtaggg
     7921 ctttctgttg aacaaccttt agcgttttat cataacaatt tactttcact catcaacctc
     7981 ttgcaatgtt gtgttgaggc taagtgcaat agttttgttt tttcatcaag ctgctctgtt
     8041 tatgctgatc ctgataaatt gcctattgat gaaaatgctc ctgttaaaac ggccaagtcg
     8101 ccttatggca atacaaaaaa aattggtgaa gaaataataa cagatactgt caggtcatca
     8161 tcactacaag ctgtttcact caggtatttc aaccccgttg gctcacacga cagtgcttta
     8221 ataggtgagc tgcctacaac aatgccactt aatgtaatgc ccatcatcac tcaggtagct
     8281 attggtaaac gaaaacagtt tgtggttttc ggaaatgatt acccaacacc cgatggcagt
     8341 tgtatcagag attatataca cgtagttgat gtagcaaaag cacatgttac tgcagtaatg
     8401 cggttactca acaaaaaaaa caagtctgct tacgaagttt ttaatctggg cacaggaaaa
     8461 ggattttctg tacttgaact tattcatgca tttgaaaaaa ccacagggca gaaattaaat
     8521 tatactattg aaggaagacg tgccggtgat gtcaccgctg tttatgccga tactgctttg
     8581 gctaacactg ttcttggatg gcataccgaa cgcgatttga atgatatgat acgcactgcc
     8641 tggaactggg agttgtatct tgccggaaga acagacttta tctgagctaa aggttcttta
     8701 ttatgtttta attttttaat tttaccctca tgtcaaaaag tacaatcatt atcactggcg
     8761 gtgccggatt cataggctcg catgttgtgc gcagatttgt aaacaattat cccgattaca
     8821 aaatttacaa cttcgacaac ctaacctatg caggaaatct tgctaacctg aaagatatag
     8881 aattaaaatc gaattatcat tttataaaag gcgacattac cgatgctgcc tttgtcaaca
     8941 aactgtttgc cgatattaat cctgatgcag taattcatct tgctgccgaa tcgcatgtgg
     9001 acagaagtat taccaatcca ctcgaattta tcatgaccaa tattgtgggt acggttaatc
     9061 ttcttaatgc tgcccgacac acatggataa aagataaaaa agtttcttca ggaaaaagat
     9121 tttatcatat cagcaccgat gaagtgtatg gttctcttgg caacaccggt ttctttacag
     9181 aaaacacttc ttatgatccg cgcagccctt attcagcatc aaaagcaagc tccgatcatt
     9241 tggtaatggc ataccatcac acctatcatt tgcctgtagt actttctaac tgctccaata
     9301 actatggctc acatcatttt cctgaaaagc tgataccgtt agccatcaac aatattaaaa
     9361 acatgaagcc tgtgcctgtt tatggtaaag gcgaaaatgt gcgcgactgg ttgtatgttg
     9421 aagatcatgc acgtgctatt gatgtgattt ttcataaggg aaaagaaggt gagaagtata
     9481 atgttggcgg caacaatgaa tggaaaaata ttgatttgat acgattgctt tgttctgtga
     9541 tggataaaaa attaaatcgc aaagaaggta catcagcatc gctcataaca tttgttaccg
     9601 acagacccgg acatgattta cgctatgcta tagattctac aaaacttcag aatgaattag
     9661 gatggaaacc atcactgcaa tttgaagaag gacttgaaaa aactgttgac tggtatcttg
     9721 ccaatgacgc atggctgcaa caggtaacct caggtgaata catgacctat taccagcatc
     9781 aatacgatgc acgataaccc caaaaaatga aagaaacaat ttatgaacgc gcctaccatc
     9841 agcagtcttt aaaagacaaa gcattcctca taaccggtgg ctccggtttt attggaagca
     9901 acattgttga atacttgtta gtgcatggtg caggaaaagt tcgtgtgctt gacaatcttg
     9961 caaacggcta caaaaaaaat atcgaacctt ttctgaaaca tccatcattt gaatttattg
    10021 aaggtgatat taccaatgct gatacatgcc tcaaggcaat gaaaggcata cagtatgttt
    10081 cgcatcaggc tgcattaggt tcggtgccac gttcgttagc caatcctttg gccacgcatc
    10141 atgccaatgc aacaggattt ctgaatatgc tttgggcagc aaaagaatcg ggtattgaac
    10201 gcattgtgta tgccagctct tcatctgtat atggcgacag tcccgaacta cccaagcgcg
    10261 aacacataac gggtaatcca ttgtcgcctt atgctgtttc aaaaatgtcg aacgagcttt
    10321 atgcctacac attctgcatg cattacgata tgaagattat aggtctgcgt tattttaatg
    10381 ttttcggccc caatcaaaat ccggataatc cttatgcagc ggcaatacct ctttttatga
    10441 aagcgctgct caaagatcaa cctgcctata tttttggtga tggtgaacaa tcgcgcgatt
    10501 ttacttttgt tgaaaatgct gtgcaggcaa acatcagagc cttattcagc gaacataaag
    10561 atgcatgtaa taaagtttac aatattgctg tgggagaaag tatcagcctt aataaattac
    10621 taaagcatct gatgcacatt acaggaaaaa aaatcgagcc tgtttttaag gaagaacgtc
    10681 cgggtgatgt aaaaaattca ttggccgata ttagtctggc aatgcaatat ctcggttata
    10741 atcctttggt aaaagtcaag gaaggacttg cacttacagt accctggttt aagaaaaact
    10801 tttgttgaag tattacaaga catataagat tagtgacacc gaaaaatagg ctgcaataaa
    10861 aaagatttaa tcatcaaata agattgttac agccataggc taaaagttta ttttaacctt
    10921 cataaattta atgaccctca ggcattttac atcacactgt aaggtttaag agtagtgtac
    10981 cccgaataat aaagtatgct tgtactttcg atttgataat gcattatccg gctacaagtt
    11041 aatttaaata gcatacatgt tatctatacg ccaactttat ctttgaaaga tgctttactc
    11101 ctgatttttc ttcgtatttt gtatttaata atcagatata cgaaaaggtt ttaaaaactg
    11161 acaatataaa atgcgaatgg ttggcttttg cattaagaca ctttgtttca attgactcgg
    11221 gttttaaacg tagaaaatta cttgcttgag aaaaaaatga atagtttcgt atatttgtga
    11281 gttggcggca agtgtaacaa cgacaaacaa acataaaata tgaagtcata tacaaacaca
    11341 gaactaaaag acatttttaa agaaacagag aagactttct tttctcaatc aacttttaat
    11401 aaatcacaac tgacagaagc tcttgagcca tttaaacaaa tcaaaaaagt aaagcgtata
    11461 gacaacgact acttttgggt tatggtgtat gttgcttttt attcgggctt taaagcgaca
    11521 actgtaacta ataaaattgg tgctattcaa gattattttg gtgacttaga caaagtatcc
    11581 aaatatgacg aagcgaaagt tcaaaaaata attgacagca ataaagtaat aaaccataga
    11641 cagaaaatca atggaataat tcataatgct aaacagatta aagcaattca aaaagaattc
    11701 ggctcgtttg acaagtatct tgaatctttt ggagacacag accaagacga caactttttc
    11761 gacttggtaa aagacttgaa aaagagattt aaatatttag gcggtataac ggtttttcac
    11821 tttttaacgg acattggatt taatgttgtt aaaccagaca gagtattatg caggattttt
    11881 aacagacttg gacttgttga cagcgaagaa gattacgccg gtgtaataaa agcgggcaga
    11941 caattttcaa ttgctaacaa agttccaatc cggtatatag acattatttt tgtagtatat
    12001 gga
//
LOCUS       DAOQNP010000085        11980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1896_length_11980_cov_8.333501, whole genome shotgun sequence.
ACCESSION   DAOQNP010000085 DAOQNP010000000
VERSION     DAOQNP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11980
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_11980_cov_8.333501"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2887)
                     /locus_tag="PKY09_10640"
     CDS             complement(<1..2887)
                     /locus_tag="PKY09_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13353.1"
                     /translation="MIKGNFRYFLLIVSLALVFSAIALEWNMHKRDLDFIRITVFAKK
                     TLHEEENRANKYVEWLGNMLSRQTSYAAIDDSLLLTSQKPVSAFYSFVYLNDSLIYWD
                     NNAVSFAYNQVKNGINQQLIQLKNGWYELFRYQRRNITIIGLLPIRAGYDFQNKYLRN
                     EFNPVLGIPEHALLLPSPATGSYPVISSDGNYLFSIKYNPIMSDPGRLWYIAALYLAG
                     FFLLLISFYLYARQWLLKNPLVSFIIISLLVFIKWLMLTFRFPEFLYGMPLFNPKFYA
                     TSYFLNSLGDFLLSASVFCCVILFVYNYLHLRRRKIRVIRQSPTLLSIVVIGNLLFTF
                     LFSVFINYLISGLILNSKISFNVNNVFELTFFSLIGFLIIGILLFSFYVSCEGTVKFA
                     EYSGFSLPYNFLLFLITQGLFLVVLILFRNTEVFINYGVATFLLTNSLILFISYIRFT
                     SRQNFSFVRYMFVIAGFSVYAAYTISSFNMVREKNNMRLLVSKVENREDLIAEYLFQE
                     IDQKLKTDPVIISNITQGRTPLLEEKIKKRITLNAFNQAYWTRYDIQIKAFDSAGVSL
                     FANSDTLRNVTFYDSIINTQTRPTYSHSFNYLSANSGKIGYIGKITYLRNASGELAGT
                     IIIQLDSKFNQEEGGFPDLLLSEKMPASRDLSKYAYAKYENGKLIFQSGKFNYFHSAA
                     TYDRMFGLIKSDQFVTLGGFIHLFYNQDKTNLIVISYKSPQAIEQVTLFSYVFIFFSA
                     SFMLIYLIWLYLANNFRLHLDFRKRIQLSVVGLVVIAILLIGGGSIFYISRGYSEEQK
                     VRIKEKLSTLMLAIESEFNDKNLTEANLTEETTLNFSRISNTLSSDFNLYDKNGRLIY
                     STQPKIFDKEIISRLMNRGAFDQLKNYQANGYIHDEKIGLLEYTSAYETIVNKDNKVA
                     AYINLPYFARQNELKKEISGFLVTLINIYVLLFGIAIVITFIISNRLV"
     gene            complement(2970..5096)
                     /locus_tag="PKY09_10645"
     CDS             complement(2970..5096)
                     /locus_tag="PKY09_10645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNB13354.1"
                     /translation="MKKKSILIFTIMSIASASFAENKDLKEQSANPLLQPWTGPYNGI
                     PAFDKVKEEYFIPAFEAAFAEKKAELQKIASNKEAPDFKNTIEAMEKAGATLNRVRAI
                     YDIWSSNLNTPTMTAIQEKIEPLIAAFNDEIVQNKPLFLRIEKVCNDTVGKKYSVEQH
                     QLLKRYYNDFVLAGAKLSDADKAKVAVINSRLATLFTQFSKNQLADENEKFIEIKNES
                     EMAGLPADLKSAYAKNAKERGKSTWTIANTRSAVEPFLTFANNRELRKQVWTMFINRG
                     DNGDANDNNKIIPEILKLRYERAKLMGFATFAHQKLSDKMAKTPEATMKLMESVWPAA
                     IARVKEEVADMQKIADKENAGIKIEPWDYRYYAEKVRKEKYDLDQNEVKQYLQLEKLR
                     EGMFFVAGELFNLSFTQVHDVPVFHPDVTVYKVEDKTTHKLKGIWYFDPYARTGKRSG
                     AWMTAYREQEKLSGNVTTIVSNNCNYIKGSGNDPVLISWDDATTLFHEFGHALHGLCS
                     DVTYPTISGTNVARDYVEFPSQVLERWLPTATVLQKYAMHYKTGHPLPQSLLEKLNNA
                     SKFNQGFETTEYLSSALVDMKIHLAGDKVTDPDQFEKETLAALNMPSEIVMRHRLPQF
                     GHIFSSDGYAAGYYSYLWSDVISADATEAFTEAGGLYDKNIANKLYRNVFSVGASIDE
                     STGYRNFRGHDPDVKALMRSRGFPDK"
     gene            complement(5224..8070)
                     /locus_tag="PKY09_10650"
     CDS             complement(5224..8070)
                     /locus_tag="PKY09_10650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HNB13355.1"
                     /translation="MLKRCIMLSFLWIAPWITSATNNTGGHFTLPAGITSEDYQQGKV
                     IFKVKDTYRSLCTATGINIQPLNVVLNNLGCTAVVKMFPGKEKPATERNAMHQLYADL
                     SLIYVAQINASASIEKSINAMLATGVLVYAEPSYIYKTNFTPNDGQIGSQWFLTKIQA
                     FSAWDTQQGDTSVVIGIVDSGTDWDHPDLQNNIKYNYADPINGVDDDNDGYIDNYRGW
                     DMSMNDNNPMVDMSDHGSHVSGCAAATTNNGTGVASPGFNCKFLPVKCANATSVTTID
                     RGYEGIVYAADHGCQIINCSWGGSAGGSVGQDAITYATVNKGALVVCSAGNTYTDIAQ
                     YPAAYNYAFSVAATNSSDEKSSFSTYNYSVDIAAPGSNIFSTLYNNSYASLDGTSMSA
                     PITSGCAAIVKSQFPTYNNMQVAEQLRMTADNIYGVSGNSGFIDKLGTGRVNLYNAVT
                     QSPKGVRMDNISLSDNNDLAFVAGDTLRITGDVLNLLAPLSNLTVTLTTVSSSVTILN
                     NTINPGAMATLDVINTATTPFLVKIKPTAPVNSNVVFKLSFTDGNLIINQYFTVVVNV
                     DYINVNISEVATTITSKGRLFYYGINQVSGLGFDFNGDNLVYDGGLMIGNNNLVSDNC
                     RDGANFDDDFQKQIAIKAIIPSVKSEFDLTTNFNDNNVTAANRLKVLVRHNTYAWSSP
                     GNSRFIIVEYHIKNTGTNALSSLYAGICADWDIQTYANNKSDVDAANKMGYTWCTDNG
                     GLYAGVKLLTATPFNSYAIDNVTGGAGGVDATSDFTSAEKYTTLSTSRAQAGNTLPAG
                     NDVIQVVSSGPFNLAGGDSITVAFALIAGDSLSDIQQSAANAQIKYDGILTSVQENIV
                     QSAETQLLNPYPNPNSGNELNIPFYIAKNQVKSVNISLTDMTGRTVVELQNIQAVSGM
                     NIQTVNMTGLSNGIYLIKLESAGKIYSGKISVQH"
     gene            8277..9026
                     /locus_tag="PKY09_10655"
     CDS             8277..9026
                     /locus_tag="PKY09_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB13356.1"
                     /translation="MINLVIVDDEVNQINLIKDCIENFFPQIRILGTANSVQEAETLI
                     RSTKPDLVFSDIDMPPYTGFDLLQRFEKIDFGVVFITGFDKYAVRAFEVAAVDYLVKP
                     VSQEDLQRSIKKFEVEAELKRSAEMLQVLLHNHNVSQKDEMHIALPKFSGFDVLKLKQ
                     VIHIRGEGNYSRFFLTDNTERLVTKQLGVYEKALTDFDFFRIHKSYMVNLSHVKSVSH
                     NNGGEVIMSNGNTIEIARGRKEDFLLRLKPF"
     gene            9252..9650
                     /locus_tag="PKY09_10660"
     CDS             9252..9650
                     /locus_tag="PKY09_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13357.1"
                     /translation="MKELKMCPITKCETGAKLIGVKIDNDIFDFLKENIVVDEDLFDV
                     MTAISNPERRFRFSNVCLESGCKHWQGESCGCLRKVLKDIENLSLSKDTTLPQCSIRE
                     RCRWFHQEGRRACDICPLLAIIDKDMIETS"
     gene            9809..11227
                     /locus_tag="PKY09_10665"
     CDS             9809..11227
                     /locus_tag="PKY09_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13358.1"
                     /translation="MKTVIVVIFVFLFSNAAKAQNTFEKVVDTLGSGGANCIMQTFDG
                     GYIYCGVCSLNGNDAIIVKLDSIGTIEWVKRYSGPGIESADYIEQLPDSGYIVNAIYD
                     GGLYSKSWLLRLDVNGDSLWTKTFSAGTGATDPSVQNSMTSVNSAIYGLTGYYKPMPP
                     TFISAFFIATAGNGFQLSNKIYSPSIYGTDSRSIEKTFDGGFVMAGVIGKPFSQSDIY
                     VIRTNTYGDTLWTKAYDLSWHEAAFDIFQTNDSGFIITGIVQNLSTFQYNLSLIKTDS
                     NGDTLWTKLYGGSLTCEGRSILQTNDGGYVVVGTINNPDPQVYILKANQNGDTIWTKE
                     FGSIAGDHGYFVKQTKDGGYIISGDGFINGVGGAYIIKTDSLGNINSGTGVAEVNNPF
                     KFNIYPNPSTGIFQVHVNGMATKTASLYIYNTTSQLVYAGNIINNKPKQINLSELASG
                     IYSLRTIIGSHQYFSKKIVIQK"
     gene            11267..>11980
                     /locus_tag="PKY09_10670"
     CDS             11267..>11980
                     /locus_tag="PKY09_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13359.1"
                     /translation="MKKQFLFVITLIVVYTSSYSQSQLNHWYISPKKMDMSLSPPIPS
                     TISPTFGGVSAATVVQVANGMYDKAGNLLFYVSDGGVYDYNNTLIGSLNNGGTEIIIV
                     PFGDNDTINNCHRKFNVFTTSGGFTSATSLWRAEVDLNSLSVTSTLVDTINYYGNTGT
                     EFGAIAVGRVTSSKRYLYFLAGPGTTNSTAGGIKKVTIKNDGSVTLNGRIYPTVANPN
                     NNTGAEVFAQELDLSPDGKW"
BASE COUNT         3771 a         2155 c         2251 g         3803 t
ORIGIN      
        1 ttacaagccg gtttgaaata ataaaggtga ttacaatggc gatcccaaaa agtagtacgt
       61 aaatgttgat tagcgtaacc aagaaaccac tgatttcttt ttttaattca ttctgtcggg
      121 caaagtaggg gaggttgata tatgcagcca ctttattgtc tttattaaca atagtttcgt
      181 aggctgaagt atattcaagt aaaccgattt tttcatcatg aatataacca ttagcctgat
      241 aatttttaag ttgatcgaag gcaccccggt tcatcaacct tgaaataatc tctttatcaa
      301 atatttttgg ttgtgttgaa taaattaatc gcccgttttt gtcatagaga ttaaaatcgc
      361 ttgatagtgt attggaaatt cttgaaaagt ttaaagtggt ttcttcggtt agatttgcct
      421 cagttaagtt tttatcatta aattctgatt caatagccag catcagtgtt gatagttttt
      481 ctttaatcct aaccttttgt tcttcggagt aacctcgtga aatataaaaa atagaaccgc
      541 caccaattaa taaaatagca attacaacta agccgacaac tgaaagttga atacgttttc
      601 tgaaatcaag atgtaacctg aaattattag ccaggtaaag ccaaatcaaa taaatcaaca
      661 tgaatgatgc agaaaagaag ataaaaacat acgagaataa agttacctgt tcaatagcct
      721 gtggcgattt gtatgaaatt acaattagat tggttttatc ctgattatag aacaaatgaa
      781 tgaatccacc taatgttaca aactgatcgg atttaataag tccgaacatt ctgtcatagg
      841 ttgcagcaga gtgaaaatag ttgaacttgc cactttgaaa aatcagtttg ccattctcat
      901 attttgcgta tgcatatttt gaaagatcac gtgatgcagg cattttttct gaaagaagta
      961 agtcaggaaa gcctccttct tcctgattaa atttagaatc gagttgaata attattgtgc
     1021 cagcaagttc accactggca tttcttaggt atgttatttt tccgatatac cctattttac
     1081 ctgagttggc actcagataa ttaaatgaat ggctataggt tggtcgtgtc tgagtattga
     1141 tgatgctatc ataaaaagtt acattacgca atgtatcgga attagcaaac aatgaaacac
     1201 ctgcagagtc aaaagctttg atttgaatat cgtagcgtgt ccagtatgcc tgattaaatg
     1261 catttaatgt gatgcgtttt tttatttttt cttccagtag tggcgtcctc ccctgagtaa
     1321 tgtttgagat aattacggga tcggtcttca gtttctgatc tatttcctga aataaatatt
     1381 cggcaatcaa atcttctctg ttttcaactt ttgaaactaa cagacgcatg ttattttttt
     1441 cacgcaccat attaaagctc gaaatggtat atgctgcata aacagaaaag cctgcaatga
     1501 caaacatgta gcgtacaaat gaaaaatttt gcctgctggt aaatcgtatg taactgataa
     1561 ataatatgag cgagttggtc agaagaaaag ttgctacacc ataattaata aaaacttcgg
     1621 tgttgcgaaa caaaattaat acgaccagaa aaagtccttg tgtgattaaa aacaaaagaa
     1681 aattataagg taaagagaag ccgctatact ctgcaaactt tactgttcct tcgcaggaaa
     1741 catagaagga gaataacagt atgccgataa ttaaaaatcc aatgagactg aaaaatgtca
     1801 attcaaaaac attatttacg ttgaaagaaa tttttgagtt gagaattaat ccacttatca
     1861 gataattgat aaatacagag aatagaaaag taaacaacag atttccgatt acaactattg
     1921 aaagtaatgt tggactttgc ctgatgactc ttatttttct tcttcttaga tgaaggtagt
     1981 tataaacgaa cagaataaca caacaaaaaa cagaggcact gagtaagaag tcacctaatg
     2041 aattcagaaa gtatgatgtt gcataaaact taggattaaa tagcggcatg ccatataaaa
     2101 attccggaaa tcggaatgtt aacatcagcc atttgatgaa aaccagaaga ctaataataa
     2161 taaaagacac caatggattt ttcaataacc actgtcgtgc atagagatag aaagaaataa
     2221 gtaagaggaa aaatccggct aaatacaatg cggctatgta ccacaagcga cccggatcgc
     2281 tcattatcgg attgtatttt atgctgaaca gataatttcc atcagagctg attacaggat
     2341 atgatcctgt agccggtgag ggtagcagta aggcatgttc cggaattccc aaaacaggat
     2401 taaactcatt tcgcaagtac ttgttttgaa aatcgtagcc tgcacgaatg ggtaataggc
     2461 caatgattgt aatatttctt cgttggtagc gaaacaattc ataccaaccg tttttaagtt
     2521 gaatcagttg ctgattgatg ccgttcttaa cctgattgta tgcaaaactt acggcattgt
     2581 tatcccaata aattaatgag tcgttgagat aaacaaagct atagaaggca ctaaccggct
     2641 tttgggatgt aagcaggagg ctgtcgtcaa tggcagcata acttgtttgg cgcgaaagca
     2701 tattgccaag ccattctaca tatttgttgg cccggttttc ttcttcatgt agtgtctttt
     2761 ttgcaaaaac cgttatcctg ataaagtcaa gatcgcgttt atgcatattc cattcaagag
     2821 caatggcaga aaataccaat gccaatgaaa cgataagcag aaaatacctg aagttgcctt
     2881 tgatcacatt ccgtaaggtt aaacaaaaca aaagtattta atcatacgaa aaatcgtcag
     2941 aatagttatg ctaataagcc tttcaggcct tatttgtccg gaaagcctct gctgcgcata
     3001 agtgctttaa cgtcagggtc gtgacctctg aaattacgat agccggtact ttcatcaatt
     3061 gatgcaccca cactaaaaac attgcgataa agtttatttg caatattttt atcgtacaag
     3121 ccacctgctt ctgtaaatgc ttcagttgca tcggcactga ttacatctga ccaaagataa
     3181 ctgtagtatc cggcagcata gccatcactt gaaaaaatat gcccaaactg tggcagtctg
     3241 tgacgcatca caatttctga aggcatattc agtgcagcta atgtttcttt ttcaaattgg
     3301 tccggatcag taactttatc acccgcaaga tgtattttca tatctactaa cgcactcgac
     3361 agatattctg ttgtttcaaa tccctgattg aattttgaag cattatttaa tttctccagt
     3421 aaactttgtg gaagtggatg accggttttg taatgcattg catacttctg taataccgta
     3481 gccgttggca accatcgttc aagaacctgt gatgggaatt caacataatc tcttgccaca
     3541 ttagttccgg aaattgttgg gtaagtaaca tcagaacata aaccatgcag ggcatgaccg
     3601 aattcatgaa acagggtagt ggcatcatcc catgaaataa gtacaggatc attacctgaa
     3661 ccttttatgt aattgcaatt gtttgataca attgttgtaa cgtttccact cagtttttcc
     3721 tgttcgcgat aagcagtcat ccatgcaccc gaacgttttc ctgtccgtgc atagggatca
     3781 aaataccaaa ttccttttaa cttatgtgtc gttttgtctt caactttata aaccgttaca
     3841 tcaggatgaa aaaccggcac atcatgaacc tgagtaaatg aaagattgaa taattctcct
     3901 gcaacaaaaa acattccttc tctcagtttc tccaactgaa gatattgttt cacctcattc
     3961 tgatccaaat cgtatttttc tttcctcact ttttctgcat agtaacgata atcccaaggt
     4021 tcaattttaa ttcctgcatt ttctttatca gcaatttttt gcatatcggc cacttcttct
     4081 tttacccttg caatagcagc aggccaaaca gattccatta gtttcatagt agcctccgga
     4141 gttttagcca ttttgtctga aagtttctga tgagcaaacg ttgcaaatcc catcagttta
     4201 gctctttcgt atcgtaattt taatatttca ggaataattt tgttgttatc attggcatca
     4261 ccattatcac cacggtttat aaacattgtc catacttgct tacgcaattc tctgttgttg
     4321 gcaaaagtta aaaaaggctc aaccgcagaa cgtgtattgg caattgtcca tgttgattta
     4381 ccacgctctt ttgcattttt tgcataggct gattttaagt cagccggtaa tcctgccatc
     4441 tcggattcgt ttttaatttc tataaatttt tcattctcat cagccaattg atttttagaa
     4501 aattgtgtaa acaaggttgc tagtcgtgaa ttgataacag ccaccttagc tttatcagcg
     4561 tcactaagtt tagcaccggc aaggacaaaa tcgttgtaat atcgttttaa caactgatgt
     4621 tgttctacac tgtatttttt tccaacggtg tcattacaca ctttttctat tcttaaaaac
     4681 agaggtttat tctgaacaat ctcatcatta aatgctgcaa tcagtggttc aattttctcc
     4741 tgaatagcag tcatggtagg tgtattcagg ttcgatgacc atatatcata aatagctctt
     4801 actcgattta atgtagctcc ggctttttcc attgcctcaa tagtattttt aaaatctgga
     4861 gcttctttgt ttgacgcaat tttctgaagc tctgcctttt tttctgcaaa tgcagcttca
     4921 aatgcaggga taaaatattc ttctttaact ttatcaaatg caggaatacc attgtaaggg
     4981 cctgtccaag gttgtaataa tggattagct gattgttctt ttaagtcttt gttttcagca
     5041 aaagaagcag aagctatact catgatagta aatattaaaa ttgatttctt tttcatctca
     5101 cagtaattaa tgattttata ttttgaagtt gtataattct cttggctaaa atagaaaaat
     5161 caaaccacac agaatattta tcctctgtgt ggtttgaaat attaactatg gtaattttaa
     5221 aaattaatgt tgcacagaaa ttttaccgga ataaatttta ccggcacttt ctaatttaat
     5281 cagataaata ccattactta agcctgtcat gttaacagtc tgtatattca ttcctgacac
     5341 tgcctgtatg ttttgaagtt caaccacagt tcttcctgtc atatctgtca gactgatatt
     5401 tacggatttc acctgatttt ttgcaatata gaatggtatg ttcaattcgt ttccgctgtt
     5461 aggatttggg tatggattca gtaattgtgt ttctgcactc tgaacaatat tttcctgaac
     5521 agaagttaaa ataccatcat attttatctg tgcattggca gcactttgct gaatgtcgct
     5581 aaggctgtca cctgctatca atgcaaaggc aaccgttatg gaatcgccac ctgcaagatt
     5641 aaatggtcct gagcttacca cctgaattac gtcattacct gcaggcagtg tgttgcctgc
     5701 ctgagcacgg cttgtgctta gtgttgtata tttttcagca ctggtaaaat cacttgttgc
     5761 atccacacca ccggcacctc cggttacatt gtcaattgca tatgaattaa atggtgttgc
     5821 tgtaagcagt ttaacgcctg cataaagtcc accattatct gtacaccatg tataacccat
     5881 tttatttgcg gcatcaacat cagacttgtt gttggcatag gtttgaatat cccaatcggc
     5941 acagatacct gcataaagtg aactcaatgc attggtgccg gtatttttaa tgtgatattc
     6001 aacaataata aaacgcgaat tgccgggtga actccatgca taagtgttat gtctgactaa
     6061 aacttttaat cggtttgcag cagtaacatt attatcgttg aaatttgttg tcaggtcaaa
     6121 ctctgatttt acagacggaa taattgcttt aatagcaatt tgcttttgaa agtcatcatc
     6181 aaaatttgca ccgtcacggc agttgtcaga aacaagattg ttgttgccaa tcatcaaccc
     6241 gccatcataa accaaattgt caccattaaa atcaaaaccc aatccagaaa cctgattgat
     6301 gccataataa aacaaacgac ctttgctggt tatggttgtt gccacttcgc tgatgttaac
     6361 attaatgtaa tctacattca ctacaacagt aaaatactgg ttaatgatta agttgccatc
     6421 agtaaaagaa agtttaaaca ccacatttga atttaccgga gctgtaggtt ttattttaac
     6481 taaaaagggt gtggtggcag tgttgataac atcaagtgtt gccatagcac cggggttgat
     6541 ggtattattc aaaatggtta cactgctcga taccgttgta agtgttactg taagatttga
     6601 aagaggtgca agaagattga gtacatcacc tgtaattcgt aaggtatcac cggctacaaa
     6661 tgcaaggtcg ttattgtcag acagactgat attatccata cgaacgcctt taggactttg
     6721 tgtaactgca ttataaagat ttacacgtcc ggtacctaac ttatcaatga aaccactgtt
     6781 gcccgaaacg ccataaatat tatcggcagt cattcgtagc tgctctgcta cctgcatgtt
     6841 gttataagtc gggaattgtg attttacaat ggcagcgcat cctgatgtaa tcggtgcaga
     6901 cattgaggtt ccatccaacg aagcataact gttgttataa agggttgaga aaatgtttga
     6961 acccggagca gcaatatcta ctgagtaatt ataagtagag aaagatgatt tttcatcgct
     7021 tgaattggtt gctgcaacac taaaagcata attataagca gcaggatatt gtgcaatatc
     7081 ggtatatgta tttcctgcag aacaaacaac taaggctcct ttatttactg ttgcataggt
     7141 aatggcatct tgacctacgc tgccacctgc actgccaccc catgagcagt tgatgatctg
     7201 gcatccatga tcggcagcat aaacaatgcc ttcataacct ctgtcaattg tggtaacgga
     7261 ggtggcatta gcgcacttca caggtaagaa cttacagtta aatcccggtg aggccacgcc
     7321 tgtgccatta tttgttgtgg cagcagcaca tccggaaaca tgcgagccat gatcgctcat
     7381 atccaccatt ggattgttgt cgttcatact catatcccag ccacggtaat tatcaatgta
     7441 tccgtcattg tcatcatcca caccattaat agggtcggca taattgtatt ttatattgtt
     7501 ctgaaggtcg ggatgatccc agtctgtacc tgagtcaaca atacctataa ccactgaggt
     7561 gtcgccttgc tgcgtgtccc atgcactaaa agcctgaatt tttgtaagaa accattgcga
     7621 gcctatctgt ccgtcattcg gtgtaaaatt tgttttgtag atgtaagatg gttcagcata
     7681 gaccaaaaca cccgtagcaa gcatggcatt aattgatttt tctatagaag cggaagcatt
     7741 aatctgcgca acataaatta aacttaaatc ggcatatagc tgatgcatag catttctttc
     7801 tgtagccggt ttttctttgc ccggaaacat tttaacaaca gccgtacagc ctaaattatt
     7861 taaaaccaca ttcagtggtt gaatgtttat accggtagca gtacagagtg aacggtatgt
     7921 atctttaact ttaaagataa cttttccttg ttgatagtct tcagatgtaa tacctgccgg
     7981 aagtgtaaaa tgacctcctg tgttgttcgt ggctgatgta atccatgggg ctatccataa
     8041 aaaggaaagc atgatgcagc gtttaagcat atagtttgtt tgattttttt ggagatgcga
     8101 atgtatgtaa aatgattatg tcattcacat tcagttaata agtgagaggc aaaaacaata
     8161 ttagtgctgt actgtttatt tgttgtattg tttcataggc ttaggttagg tactcaggtt
     8221 gcaaaatatt ttttgcgacc tgagtttttt tgtcagattt cttctacttt taagccatga
     8281 taaaccttgt gattgttgat gatgaagtca atcagattaa tctgataaaa gactgcatag
     8341 aaaatttctt tccacagata agaattctag gcacagccaa ctctgtacaa gaggcagaaa
     8401 cactcatccg cagcacaaaa cccgatttgg tttttagcga tattgacatg ccgccataca
     8461 caggcttcga cctgttgcaa aggtttgaaa aaattgattt tggtgttgtt tttatcaccg
     8521 gttttgataa gtatgctgta cgtgcttttg aagttgcagc tgttgattat ttggttaaac
     8581 ctgtaagcca agaggacctt caaaggtcta taaaaaagtt tgaggtagag gcagagctta
     8641 agagatcagc tgaaatgttg caggtattac ttcacaatca caacgtaagt caaaaggacg
     8701 aaatgcacat tgcccttccc aaattttcag ggtttgatgt actcaagtta aagcaggtga
     8761 tacatatcag aggggaaggc aattattccc gcttttttct tacagacaat acagagcgtt
     8821 tggtcactaa acaattaggc gtttatgaaa aagcactcac tgattttgac ttttttcgaa
     8881 tacataagtc atatatggtt aacttgtcac acgtaaagtc tgtaagccac aacaatggtg
     8941 gagaagtaat catgtcaaac ggaaatacca tagagattgc ccgaggcaga aaagaagatt
     9001 ttttattgag attaaagcct ttttaatagc tactatcttt cgtacaacca catctgcatt
     9061 tgatacaaaa ccattaggtt gttaaattaa ttgtaattta ctttgaagtc atatcctcaa
     9121 ctcggatttg gaaatcgccc tgaggctaac ccaattgaaa aaatctgatt cccggtgtta
     9181 acgaataccg ggatcagaaa aatttaaaca gtgaaaaaag taaatattac aaaaccgcaa
     9241 aaattaaaga catgaaagag ttgaaaatgt gccccataac caaatgcgaa accggagcaa
     9301 agcttatagg tgtaaaaatt gataatgata tatttgattt tttaaaagaa aacatcgttg
     9361 tggatgaaga cctgtttgat gtgatgactg caatcagcaa tcccgaaagg cggtttcgtt
     9421 ttagtaatgt ttgcttagaa agcggatgca agcactggca gggagaatcg tgtggatgtc
     9481 tgcgaaaggt gcttaaggat atagaaaacc tgtcattgag taaggacacc actttaccac
     9541 aatgcagtat tcgcgaaaga tgcaggtggt ttcatcagga aggacgcagg gcctgtgata
     9601 tatgcccact attggcaatt atagataaag atatgatcga gacatcataa gctctattct
     9661 gtacataaaa atctaaattg tgttttcatt tggtttgtta tgctacaagt agtcatgatg
     9721 ctttttgaaa tttttataaa tggttatcct ctataatgac agattgattt tttatcttta
     9781 cactgcaatt ttaaataagc cataaccaat gaaaacagta atagttgtaa tatttgtttt
     9841 tttattttct aacgctgcta aagcccaaaa caccttcgaa aaagtagttg atacattggg
     9901 tagtggtgga gcaaattgta ttatgcaaac ttttgacggg ggttacattt attgtggtgt
     9961 atgctctttg aacggcaatg atgccattat tgttaaactt gattctattg gaactattga
    10021 atgggtaaaa agatatagcg gaccaggaat tgagtctgcg gattatatag aacaattgcc
    10081 cgacagtggc tatatcgtaa atgctatata tgatggggga ttgtattcaa aaagctggct
    10141 tttgcggctt gatgtaaatg gcgattcgct ttggacaaaa acattttctg ctggcacagg
    10201 tgctacagat ccatcggtgc aaaatagtat gacttcagtt aatagtgcaa tatatggact
    10261 aacgggttac tataagccaa tgcctcctac atttatcagt gcttttttta ttgcaacagc
    10321 tggaaatggg tttcaattgt caaataaaat ttatagtcct tcaatttatg gcactgattc
    10381 tcgttcaata gaaaaaacat ttgacggtgg tttcgtaatg gcaggtgtta ttgggaaacc
    10441 tttttcgcaa tctgatattt atgtaatccg aaccaatact tatggtgata ccttgtggac
    10501 aaaagcttat gacctttcat ggcatgaagc agcatttgat attttccaaa ctaatgacag
    10561 cggttttata ataacgggta tcgttcagaa tttatcgaca tttcaatata atttgagttt
    10621 aatcaaaact gattctaatg gagacaccct ttggacaaag ctttatggcg gttcacttac
    10681 ctgcgaagga cgttcaattc ttcaaacaaa tgatggaggg tatgttgttg tgggtaccat
    10741 aaataaccct gatcctcaag tttatatttt aaaagctaat cagaatggag atacaatatg
    10801 gacaaaagaa tttggtagca tagcaggtga ccatggttat tttgtcaaac aaaccaagga
    10861 tggcggatac atcattagtg gtgatggctt tattaatggt gttggaggtg cttacataat
    10921 aaaaaccgac agtttaggaa atattaattc cggaacgggt gttgccgaag taaataaccc
    10981 atttaagttt aatatttatc ccaatccttc aaccggaatt tttcaggtac atgttaacgg
    11041 tatggcaacg aaaacggcat ccctgtatat ttataatacc acatcgcaac tagtttatgc
    11101 cggaaacata ataaataata aacctaaaca gattaattta tccgaattgg cttccggtat
    11161 ttactcactc agaaccatca taggaagcca tcaatatttt tcaaaaaaaa tagtcattca
    11221 aaagtaaatc aacaattatc agtctaacta taaaaaacga aataaaatga aaaagcaatt
    11281 tttgtttgtt atcacactta ttgtagttta tacatcaagt tactcgcaaa gtcaattgaa
    11341 ccactggtac atttcgccaa aaaagatgga catgtccttg tccccaccga ttccatctac
    11401 aatctcgcct acttttggtg gagtttctgc tgctactgtt gtacaggttg ctaatggcat
    11461 gtatgacaaa gcgggcaatc ttttatttta cgtgtcagat ggaggcgtat atgattacaa
    11521 caatacactt ataggctctc ttaataatgg tggaacagaa ataataatag ttccatttgg
    11581 agataacgat accattaaca attgccatcg gaagtttaac gtttttacaa catctggagg
    11641 ttttacaagc gcaacaagtt tgtggcgtgc ggaggtagat ttgaacagtt tatctgttac
    11701 ctcaacactt gttgatacaa taaactatta tggcaataca ggaactgagt ttggagctat
    11761 tgctgtgggt agagtaacct caagcaaacg atatctttat tttttagcag gcccgggtac
    11821 aacaaattca acagcaggag gaataaaaaa agtaaccatc aagaatgatg gttctgtaac
    11881 ccttaacggt cgtatttatc ccacggttgc aaatcctaac aataataccg gtgcagaagt
    11941 gtttgcacag gaattggatt tgtcgccaga tggaaaatgg
//
LOCUS       DAOQNP010000086        11902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_1911_length_11902_cov_8.425509, whole genome shotgun sequence.
ACCESSION   DAOQNP010000086 DAOQNP010000000
VERSION     DAOQNP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1911_length_11902_cov_8.425509"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKY09_10675"
     CDS             complement(<1..328)
                     /locus_tag="PKY09_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNB13360.1"
                     /translation="MFGQRITLFYFFFVLGFHAVYAQQQRVTGKVTDDAGALPGVAVY
                     TDENHATTTDANGDYILMLDKGSWTLKAKLLGYETFQKVILVGENNFVQIHLIPLSKQ
                     LQSVVVS"
     gene            399..1523
                     /gene="dprA"
                     /locus_tag="PKY09_10680"
     CDS             399..1523
                     /gene="dprA"
                     /locus_tag="PKY09_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNB13361.1"
                     /translation="MENNSTTDVLQYQLALTLLPGVGDITAKNLISYCGGAEAIFKEK
                     QQALLKIPKIGQIVTDKIKNGKVQSLKRAEEEIRFIEKNKIKALFFTSDLYPKRLKSC
                     NDSPIMIFVKGNANLNPEKCIAFVGTRNATDYGKMITEKIISDLAAIDGLMTISGLAF
                     GIDITAHKMSIKNNIPTIGIVAHGLDMLYPAEHRNTAEKMLEAGAVISEFMSKTNADK
                     ENFPKRNRIIAGMADAVVVVESAIKGGSLITADIANSYNRDVFAVPGQVDREYSKGCN
                     ALIKDNKANLIQSAADIEFFMGWDKKDQKSVPKQARLFEDLDAEEKQLVEFLKENAKS
                     DIDTICFRSGVKLNRIPALLLNLELKGVIRNYPGKVYDLL"
     gene            complement(1573..1845)
                     /locus_tag="PKY09_10685"
     CDS             complement(1573..1845)
                     /locus_tag="PKY09_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13362.1"
                     /translation="MKKVLALVMAAGMFSIVACGPSAEEKQKMEETAKAAQDSIAAAA
                     QQAASAAEQAATDAAQVATDAAQTVDSTAGAVVDQATEAVKEATTK"
     gene            2074..2676
                     /locus_tag="PKY09_10690"
     CDS             2074..2676
                     /locus_tag="PKY09_10690"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNB13363.1"
                     /translation="MSFQLPPLPYAPNALEPHIDARTMEIHHGKHHQAYVTNLNNAIA
                     GTDAEKMSIEDICKNISKLPMAVRNNGGGHYNHSLFWTIMAPNAGGTPKGKLAEAINS
                     AFGSFDEFKTKFNQAAATRFGSGWAWLIKNAAGKLEVCSTPNQDNPLMDIAEVKGTPL
                     LGIDVWEHAYYLHYQNRRPDYCTAFWNVVNWDEVSKRFEK"
     gene            2865..5333
                     /locus_tag="PKY09_10695"
     CDS             2865..5333
                     /locus_tag="PKY09_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNB13364.1"
                     /translation="MNKYLRYFVFLLLTCLALLLIGMGLSVFLQDKIKQSVIEEINKS
                     LNTPASVSEIDFSVLRFFPNASITLKNVVIKGNPWQDTKRPLLKAGKIDLVLSITSIF
                     SSTMQLKKLEISDAELNILTNEKGQKNDQIFKQGNDNNNFAAEFKKVYLKNVNLLIEN
                     KPTNFILQTHLNKGLATGNFKDKIFELKTETDLFAEVIQHENIAYIKQKPIELSGVIS
                     VDLRNDIYAFNDLKIQLANLNIKAKGKIEKRNETFLDLNFTSSKANASELLSILPTNW
                     VAPQILNYRYHGNIKFDTHIYGIASSNQSPTIEINFGTDKTDIIPDNENYALKKVALK
                     GFYTNRKSGKNVSVLSLKNISANIEGKQLKGEVYLENLKNPYFNIQLDADISLASLSR
                     YLPADFTEEQQGSISLHGRISGQADKKQTYRSNGTLTLNNLKFKLKKRNIAVTGINGI
                     IYFNNADVKVESLKILAGQSDIMINANILNFYNYIFLDNQIITINGIVESHFLDAGEL
                     LAGNLSADTSDFDLPAYLNLNASLSADIVKFRKFNAKDLSGNISLKDKILNAKQLSFN
                     AMEGHIELDGTINSNFKDEVHISSDAKIESINIERLFYETGNFGQQLLSDKNIRGTLT
                     ATVQLATYWTKQLEVKSNRTIASANLTINDGALIDFKPMLKLSRFVKGSDLQNVRFSK
                     LQNTIRINEQKILIPTMDIRSTAMNMTISGTHGFDNFVDYNIQMKLSQLLGKKVRQQN
                     TEFGTIEDDGSGGLNLFLTMKGPLDNPRFSYDRKGVEKKITESVKSGKDDFLKIIKEE
                     ISGKKPQKEETPKKQKELQIDYDE"
     gene            5494..7425
                     /locus_tag="PKY09_10700"
     CDS             5494..7425
                     /locus_tag="PKY09_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13365.1"
                     /translation="MKNKIIVLTLISAAILGISAIPFQPKKAMAYHSESEIEFFKNHS
                     MTPIAPGEYFLTPENCKGCHGFDTLGIANVDANGHDVNLFDDWETSMMGLSAVDPFWR
                     AKVSHETMVNPAHATELQNLCITCHAPAGHYSAIYKGQQHYVMSDLLNDSLGLSGVSC
                     TACHAIGDSSSLGQLFTGQIPYDTNKVLFGPFTAPVAGPMQLYVGMMPTYSPHVSEGK
                     FCSPCHTLLSNTVDLSGVPTGGTFIEQATYHEWLNSAYPSQATVCQSCHMPQIEDAVI
                     LANGNVGLPGRTPFNLHQFAGANSFMVNLIKQNKTTLGVSAPDYNFDSTLAAITNMLT
                     KQSLDVQASLVSITSDTAFIDVDLTNKAGHKFPSGYPSRRAVLQMIVTKANGDTLFAS
                     GLFDANYEMQNVATPFEQHHDIINTQTQNQIYEMVMGDVNGTVTTVLERAASHLKDNR
                     IPPAGFSTLHYAYDTCKIVGSALTDVDFNKNGVTQGTGHDIVHYHIPLNNYSGTINVY
                     TAVYYQTLPPAFLSDMSNYSSPEINSFLTMYANADKSPVKVDADTLLNISTGIKENTL
                     TTINISPNPTRDGKVLLSGIGYNSFQLEVFDQNSKKINVILTRKSNSIELQLPTAKGL
                     YFIRLNVNGQVKSYKILRI"
     gene            7485..10352
                     /locus_tag="PKY09_10705"
     CDS             7485..10352
                     /locus_tag="PKY09_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNB13366.1"
                     /translation="MKNKLYSKLGCLLLIAPAFSFAQLSFTNSNSRLASPAFHSGCPV
                     SVVDWNNDGLDDIIRLDDGRDCYVDVHKTNQQYERIFLGSFSGGAWAMAVADLDKNGY
                     KDVVAGGSFGSVQVLKTNNSGTGATLSSLNNSNFFLQNLTMADFDNDGWVDIFCCDDN
                     AASHIYLNNGSGGFGAQSNIINFNLPNGSDNSGNYGSVWTDFDNDGDLDLYIAKCRQS
                     VQNPTDPRRINVLFVNNGNGTFTENAAAYNINIGWQTWTAAFGDIDNDGDLDLMITNH
                     DHNSQIFQNDGTGVYTDITASTGFTVSDITPIESVMEDFDNDGFVDIFVTGSDSRFWR
                     NNGNGTFTKIEGLFNNNDMESFAIGDANHDGWIDVMASYAAIYTTPTSIDDVLWINNG
                     SKQNIIDGIAGLSTNNFFNVVLEGNATNKDAIGARALIYGPWGVQLREVRAGESYGTN
                     NSAILHFGLGTATAIDSMKVIWPSGVSQTVSNPSINQFLSVKENDCVSPEAIVTASGP
                     LVLCTSGQTLTLTAPAGFNYLWSDSTTAQSITITQTGEYNVTISQPGNNCSATSATLV
                     VIPSPDQTPAITALGETVFCNGGSVQIQGPVGFSSYLWSNGDTTQTATITTNGPVTLT
                     IQGICQSYTSDPINVTVNTVTDPAANDVTLSAAGSATLTATIGNNISWYATNAGGTPL
                     ATGQSYTTPFLTANTTYYMQATDTFGGGIYAVGLPTWSGAGSGYSGSTTNATTEFDVT
                     KNCTLKSVKVFTNAAGIRRIELKDASSNVLNYVDVNVSPDSQVVTLNFPLTPGMGYTL
                     GTNTTTNQNNLGTTSPKFKRNSSGVNYPYSLSDALNITNSSNGGQYFYYFYDWQVEKE
                     SWLCESNMVPVNVYITTGISEHNNELLSTYPNPVSDQLHIYNPFGTTKATLLDITARV
                     VNTYMLNNGENSFDIASLNAGVYHIQVENEGVITNLKFVKN"
     gene            complement(10506..11588)
                     /locus_tag="PKY09_10710"
     CDS             complement(10506..11588)
                     /locus_tag="PKY09_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNB13367.1"
                     /translation="MDAYKPFEPQCYNFEIGTHQNKKVIWIAFPYNTKLIQHLKQHTS
                     AQWSRSNKKWYVLDKTSYRKLFGIEAMVMSSHVLADVNAVNHSAFNRFVEQLQLKGYS
                     PNTMRTYSTEFVQLLKVIKTTKAESLSSEKLRSYFLYCIKTLKLSENTIHSRMNAIKF
                     YYEQVLKRKKVFIEVPRPKKPSTLPKTLSAGDIRKMFSAVENKKHLLILKLCYGMGLR
                     VSEIVNLKIADIDSKRMQVLISRGKGKKDRYAILPNSVLELLRAYYKEYKPTKYLFEG
                     QDGGQYNIRSVQLMFKNTMRKAKIYKKIGVHGLRHSFATHLIEQGTDIRFVQDLLGHN
                     NIKTTMIYTSLTDQTRRKIKSPLDNL"
BASE COUNT         3896 a         2192 c         2158 g         3656 t
ORIGIN      
        1 cactcactac taccgactgt aattgtttac ttaaaggtat gagatggatt tgaacaaagt
       61 tgttttcgcc aacgagtatt actttctgaa aagtttcgta gccaagtaat tttgctttta
      121 gagtccaact ccctttgtcg agcatcaaaa tataatcccc attggcatct gttgtcgtag
      181 cgtggttttc atctgtataa actgctacac cgggcaatgc acctgcatca tcggttacct
      241 taccggtaac acgttgttgc tgtgcataga cagcatgaaa tcctaatacg aaaaaaaaat
      301 aaaataaagt tattctttgg ccaaacatgt ttttctttgc agattcagat tgtaaaggca
      361 caaacctaaa ctattcttga aaatcttgaa agtcaacaat ggaaaataat tcaacaaccg
      421 atgttttgca atatcagctt gcactgacac tcctaccggg tgttggcgac attacagcaa
      481 aaaatcttat cagttattgt ggtggcgcag aagctatttt caaagaaaaa caacaggcat
      541 tacttaaaat accgaaaata ggtcagatag tgactgataa aattaaaaac gggaaagtac
      601 agtcgttaaa aagagctgaa gaggaaattc gtttcattga aaaaaataaa atcaaagctt
      661 tgttcttcac ttctgacctt tatcccaaaa ggcttaaatc gtgtaatgat tcgccaataa
      721 tgatttttgt taagggaaat gccaatctca atcctgaaaa gtgtattgct tttgtaggga
      781 caagaaatgc aaccgactat ggcaaaatga taactgaaaa aatcatatca gatttggctg
      841 ccattgatgg actgatgacc attagcggac ttgcttttgg tattgatata actgcacata
      901 aaatgagtat aaaaaacaat attccaacta ttggaattgt agctcatggg cttgatatgt
      961 tatatccggc agaacatcgc aacacagcag aaaaaatgct ggaggcaggt gccgttatca
     1021 gcgagtttat gagtaagact aatgccgaca aagaaaactt tccaaaaaga aaccgcatca
     1081 ttgcaggtat ggctgatgct gttgtagtgg ttgagtcagc cattaaaggt ggtagtctga
     1141 ttacagcaga tattgccaac tcctataaca gagatgtgtt tgcagtgccc ggacaggttg
     1201 acagagagta ttccaaagga tgcaatgcgc tgataaaaga caataaagca aatttaattc
     1261 agtctgcagc cgatattgag ttttttatgg gatgggataa aaaagatcag aagtctgttc
     1321 ctaagcaggc aagattgttt gaagatttgg atgcagaaga aaaacagctg gttgaattcc
     1381 ttaaagaaaa cgctaaatca gatatagata ctatttgttt caggtcgggt gttaagctca
     1441 atcgcattcc tgccttgctg ctcaaccttg agttgaaagg tgtgattaga aattatccgg
     1501 gaaaagttta tgatttgcta taaaaaagaa aagcccgcta aagcgggctt gtatcttcta
     1561 aaatggggct gattactttg tagtagcttc tttaacagct tcagtagcct gatctactac
     1621 agcacctgca gttgaatcta cagtttgtgc agcatcggta gcaacctgag cagcatcagt
     1681 agcagcttgt tcagcagcag aagcagcttg ttgtgcagca gcagcaattg aatcctgagc
     1741 agctttagct gtttcttcca ttttttgttt ttcttcagca gaaggaccgc atgcaacgat
     1801 gctgaacatt ccggcagcca taactaaggc aagaactttt ttcattgttt tgtaattaat
     1861 tgatttgaaa tttgagcggc aaatataaat cgctttaccg ctgtaaatta agttttatat
     1921 taaattttta ctcgctgaat atcaatgtat taaagcttgc tgcatagctt aacaactccc
     1981 acatctatgt atttcttctg aaaatagtac gtgactgaca acaaaagtag cgaaatatac
     2041 tacctttgtt gcttaacaat taaattaaaa attatgtctt ttcaactccc tccactacca
     2101 tacgcaccca atgcgttaga gccacatatt gatgcccgca ccatggaaat acaccatggc
     2161 aaacaccatc aggcttatgt aaccaactta aacaatgcta ttgcaggcac cgatgccgaa
     2221 aaaatgagca ttgaagatat ttgtaaaaat atttcaaaac taccgatggc cgttcgcaac
     2281 aatggtggcg gacattacaa ccactcactg ttttggacta ttatggcacc caatgctggt
     2341 ggcacaccaa aaggaaaatt agccgaggct attaatagtg cttttggctc gtttgacgaa
     2401 tttaaaacta aatttaatca ggctgcagct actcgttttg gctcgggctg ggcatggtta
     2461 ataaaaaatg ctgccggcaa actcgaagta tgctcaactc ccaatcagga taatccactc
     2521 atggacattg ctgaagttaa aggtacaccg ttattaggta ttgatgtttg ggaacatgcc
     2581 tactacctgc actaccaaaa ccgaagacct gattactgta ctgctttttg gaatgtggtt
     2641 aactgggatg aagtatcaaa acgtttcgag aaataatttc ctttttaatt aatttaaggc
     2701 tgtcttaatc caattgaggc agctttttta ttgacattaa aagtttaaaa ctccataaaa
     2761 caaacaccct attccgtagt ttactatatt ctaataaaat tactttgcaa aatacatttt
     2821 aatcttcact acaatttaaa aagactgcag cgttttaaga ggacatgaat aagtatctgc
     2881 gttactttgt ttttttactg ttgacctgct tggcactgct gctcattgga atgggccttt
     2941 cagtcttttt acaggataaa atcaaacaat ctgtaataga agaaattaat aaaagcctaa
     3001 acacccctgc aagtgtaagt gaaattgatt tttccgtatt gcgttttttt ccaaatgcct
     3061 ccataactct taaaaatgtt gtcataaaag gcaatccctg gcaagacaca aaacgtccgc
     3121 tgctaaaagc cggaaaaatt gatttggtgc ttagcattac cagcatattt tcttcaacaa
     3181 tgcaactgaa aaaattagag atttctgatg ctgaattaaa tattctgact aatgaaaagg
     3241 gtcaaaaaaa cgatcagatt tttaaacaag gtaatgacaa taacaacttt gctgctgaat
     3301 ttaaaaaagt atatcttaaa aatgtaaatc tgcttattga aaacaaacca acaaatttta
     3361 ttttacaaac gcatctgaac aaaggacttg ctaccggtaa ttttaaggac aaaatctttg
     3421 agctcaaaac agagactgat ttgtttgcag aagttattca gcatgaaaat attgcttaca
     3481 tcaaacaaaa gccaatagaa cttagtggtg ttatttccgt tgatttacgt aatgacattt
     3541 atgcctttaa cgatttaaaa attcaacttg ccaatctgaa tataaaagca aaaggaaaaa
     3601 ttgaaaaaag aaatgaaact tttctcgatc ttaattttac ttcttcaaaa gccaatgcat
     3661 ctgaattgct ttctattttg ccaactaact gggttgctcc acaaatttta aactatcgtt
     3721 atcatggtaa cataaaattt gatacgcata tttatggcat tgcatcatca aatcaatcgc
     3781 caaccattga aattaacttc ggtaccgata aaaccgacat tatccctgat aatgaaaatt
     3841 atgctttgaa aaaagttgca ttaaaaggat tctacacgaa tcgtaaatca ggaaaaaatg
     3901 ttagtgtctt aagtcttaaa aatatttctg ccaatattga aggtaagcag ttaaaaggcg
     3961 aagtctatct tgaaaatctt aaaaatccat atttcaacat acagttagat gctgatatta
     4021 gtcttgccag tttgagtcgt tatttacctg ctgactttac agaagaacaa caagggtcta
     4081 tttctcttca tgggagaatc agcggacaag ctgataaaaa acaaacttat cgttcaaatg
     4141 gtacactgac attaaacaat ctgaaattta aacttaaaaa aagaaacatt gcagtcactg
     4201 gtatcaatgg tataatttat tttaataatg ctgatgtaaa agttgaatct ctaaaaattc
     4261 ttgccggtca aagtgacatt atgatcaatg ccaatatact taatttttac aactatatat
     4321 ttcttgacaa tcagattatc actatcaacg gaattgttga aagtcatttt ttagatgccg
     4381 gtgaattact tgcgggtaat ctttctgccg acacttctga ttttgatttg cctgcatatc
     4441 ttaatctgaa tgcatcgtta agtgcagata ttgtaaaatt cagaaaattt aatgctaaag
     4501 atctttccgg aaacatatca ctaaaagata agattctaaa tgcaaaacaa ctttcattta
     4561 atgctatgga aggacatata gaacttgatg gcactattaa ctcaaatttt aaagacgaag
     4621 tacatatttc aagcgatgcc aaaattgagt ccatcaatat tgaaaggctt ttttatgaaa
     4681 caggaaattt tggacagcaa ttgctatcag ataaaaatat cagaggaacg ttaactgcaa
     4741 ctgttcaact tgctacatat tggacaaaac aacttgaagt gaaatcgaac cgcaccatag
     4801 catcggcaaa ccttactatt aatgatggtg ctcttattga ttttaagcca atgcttaaac
     4861 tttcgcgttt tgtaaaaggt agcgacctcc aaaatgttcg tttcagcaag cttcaaaaca
     4921 cgattcgtat taatgaacaa aaaatactta tccccacaat ggatattcgc tccactgcaa
     4981 tgaatatgac cattagcggc acacatggct ttgacaactt tgtagattat aatatacaaa
     5041 tgaaactttc tcaactactt ggtaaaaaag tgcgtcagca aaacactgaa tttggaacca
     5101 ttgaagatga tggtagtggt gggctaaatc tttttctgac catgaaaggg ccattagata
     5161 accccaggtt cagttatgac cgtaaaggag tagagaaaaa aattacagaa tctgtaaaat
     5221 caggaaaaga tgatttttta aagattatca aagaagaaat tagcggtaaa aagcctcaaa
     5281 aagaagaaac ccccaaaaaa caaaaagaac ttcagattga ttatgatgag taattgaacg
     5341 ttttttagtc attctacccg cattttttta ctttcattac aaaccaattg gcgttaataa
     5401 tttgatgttt tgccaaagat ttctattgta acacattaaa aaaattgata ttattgccat
     5461 caatccgtaa aaaatattgg ggacactgca ggcatgaaaa acaagattat cgttttaact
     5521 cttatttcag cagccatttt aggtattagt gctattccat ttcagccaaa aaaggcaatg
     5581 gcctatcatt ctgaatctga aattgagttt tttaaaaatc attccatgac gcctattgct
     5641 ccgggagaat attttttaac tccggaaaac tgcaagggct gtcacggttt tgatacatta
     5701 ggaattgcaa atgttgatgc caacggtcat gatgttaatc tttttgatga ctgggaaaca
     5761 tcaatgatgg ggctttctgc tgttgatcct ttttggcgtg ctaaggtaag tcacgaaact
     5821 atggttaatc ctgcacatgc tactgagttg cagaatttat gcattacatg tcatgcacca
     5881 gccggacatt atagtgccat ttataaagga caacaacatt atgttatgtc cgatttatta
     5941 aacgattcac ttggtctttc aggtgttagt tgtactgcat gtcatgcaat aggtgatagt
     6001 agcagtttag gtcagctttt tacaggtcag attccttatg atactaacaa agtactattt
     6061 ggacctttta ctgcgcctgt ggccggacct atgcaacttt atgtgggcat gatgcctacc
     6121 tacagtccgc atgtaagtga aggaaaattt tgttcaccct gtcacacatt gctttccaac
     6181 acggttgatt taagtggtgt tccaacaggt ggtactttca ttgagcaggc aacttatcat
     6241 gagtggctta attccgctta tccatcacag gcaacagttt gtcaaagctg tcacatgcct
     6301 caaatagaag atgccgttat tttagccaat ggcaatgtgg gcttgccggg tcgtacacct
     6361 tttaatctgc atcagtttgc aggtgccaat tcttttatgg taaacctgat aaaacaaaac
     6421 aaaactacgt tgggtgtttc tgcacctgac tataattttg attctactct tgcagctatc
     6481 acaaacatgt tgacaaaaca atcattggac gtgcaggctt cattagtttc tattacttca
     6541 gatacggcat tcattgatgt tgacttaact aacaaagcag gacataaatt cccaagtgga
     6601 tatccttcaa gaagagctgt gcttcagatg atagttacaa aagctaatgg cgatacgttg
     6661 tttgcttcag gattatttga tgccaattat gaaatgcaaa acgtagctac accttttgag
     6721 caacaccatg atatcattaa cacacaaaca caaaatcaga tttatgaaat ggtgatgggt
     6781 gatgtgaacg gaactgttac cactgttctt gaacgtgctg cttcacacct gaaagataat
     6841 cgtattcctc cggcaggatt ttcaaccttg cattatgctt acgatacctg taaaattgtt
     6901 ggtagtgcgt taacagatgt ggactttaat aaaaacggtg taacacaagg taccggtcat
     6961 gacattgtgc attatcatat tccattaaac aattacagcg gtactataaa tgtttatact
     7021 gctgtttatt atcaaacact tccacctgca tttttaagtg atatgagtaa ctattcttct
     7081 cccgaaataa actcatttct taccatgtat gccaacgcag ataaaagtcc tgtaaaagtt
     7141 gatgctgata ccttactaaa tatctcaaca ggaataaaag aaaatacctt aacaaccata
     7201 aacatttcac caaatccaac aagagatgga aaggttcttc tttcaggaat cggttacaac
     7261 tcatttcaat tagaagtatt tgatcaaaac agtaaaaaga ttaatgttat tcttaccaga
     7321 aaaagtaatt caatagaact tcagctacct acagcaaaag ggctttattt tatcagactt
     7381 aatgtgaatg gtcaggttaa atcttataaa atattacgaa tttaaaaacg aaaacatctt
     7441 ataaaaataa aaacaaatta cattaaatca gaaacttgtt taaaatgaaa aataaacttt
     7501 actcaaaatt aggatgcctg ctacttatag cacctgcatt ctcctttgca cagctttcat
     7561 ttaccaacag caactcaaga ctggcatcac ctgcatttca cagtggatgt cctgtttctg
     7621 tggttgactg gaataatgat ggcttagacg acattattcg tttagatgat ggaagagact
     7681 gctatgttga cgtgcataaa accaatcaac agtatgaacg tatttttctt ggttcgtttt
     7741 caggtggtgc atgggctatg gcagttgccg acttagataa aaacggatac aaagatgttg
     7801 ttgccggtgg ctcttttgga agtgtccagg tattaaaaac taacaatagc ggtacaggtg
     7861 ctaccctatc ctcattaaac aattcaaatt tcttcttgca aaatttaact atggcagatt
     7921 ttgacaacga tggctgggta gatatttttt gttgtgatga caatgcggca agtcacattt
     7981 atctgaataa tggttcagga ggctttggtg cacagtcaaa tattatcaat tttaacttac
     8041 ctaatggttc tgacaactcc ggaaactatg gaagtgtgtg gaccgatttt gataatgatg
     8101 gtgatttaga tttatatatt gcaaaatgtc gtcaatcggt tcaaaatcct actgacccaa
     8161 gacgtatcaa tgtacttttt gtaaacaacg gcaatggaac ctttactgaa aatgctgctg
     8221 cttacaacat caacattggc tggcaaacat ggacagccgc ttttggtgat attgacaatg
     8281 acggtgacct tgatttgatg attaccaatc acgatcacaa cagtcagatt tttcagaatg
     8341 atggaacagg tgtttataca gatattacag catcaacagg atttacagtt tctgatatta
     8401 cgccaattga aagtgtgatg gaagattttg acaacgatgg ctttgtggac attttcgtta
     8461 ccggaagcga ttcacgcttt tggagaaata atggcaatgg tacatttacc aaaattgaag
     8521 gtttgtttaa taacaatgac atggagtcgt ttgccattgg tgatgccaac catgatggat
     8581 ggattgatgt tatggcaagc tatgctgcta tctacactac acccacctca atagatgatg
     8641 tactttggat taacaatggc agcaaacaaa atattattga cggtatagca ggattatcta
     8701 ccaacaactt ttttaatgtg gttcttgagg gtaatgcaac caataaagat gctataggtg
     8761 cacgtgcatt gatttacgga ccatggggtg ttcaacttcg tgaagtacgt gcaggcgaaa
     8821 gttatggcac caacaacagt gcaattctac attttggatt aggaacagcc actgcaatag
     8881 attctatgaa agttatctgg ccaagtggtg taagccaaac agtaagcaat ccttcaatca
     8941 atcaatttct atcagttaaa gaaaatgatt gtgtttctcc ggaggcaatc gttacagcat
     9001 ccggcccttt agtattgtgt acaagtggtc agacattaac tttaacggca cctgcaggat
     9061 ttaattattt gtggagcgac agtacaactg cacaaagtat taccatcacc caaacaggtg
     9121 aatataatgt aacaatctca cagcccggaa ataattgttc agcaacatca gctactttag
     9181 ttgtcatacc atcaccagat cagacacctg ctattactgc acttggagaa actgtgtttt
     9241 gtaatggagg cagtgtacag attcagggac ctgttggatt cagcagctac ctttggtcta
     9301 atggagatac gacacaaaca gctactataa ccactaatgg cccggttaca ttgacaattc
     9361 agggtatttg tcagtcatat acttctgacc cgattaatgt tactgtaaat acagttactg
     9421 atcctgcagc aaacgatgtt actttatctg ctgccggcag tgcaacctta actgctacaa
     9481 ttggtaataa tatttcatgg tatgctacca atgcaggtgg aacaccattg gctaccgggc
     9541 aaagctatac caccccattc cttacagcga acactaccta ctatatgcag gcaaccgata
     9601 ctttcggtgg tggcatttac gcagtaggtt taccgacatg gtcgggcgca ggctcaggat
     9661 actccggaag cactaccaat gccacaacag aatttgatgt taccaaaaac tgtactttga
     9721 aatcggttaa agtatttaca aacgctgccg gaatccgaag aatagaatta aaggatgcat
     9781 catcgaatgt tctcaattat gttgatgtaa atgtctctcc tgattcgcag gtagtaacgc
     9841 taaacttccc attaactccg ggaatgggtt atacactcgg cacaaatact acaaccaatc
     9901 agaacaacct tggaaccact tctccaaaat tcaaaagaaa cagttccggt gtaaactatc
     9961 cgtattcatt atctgatgca ctaaacatta caaactcaag taatggtggt cagtacttct
    10021 attacttcta tgactggcag gtagaaaaag aatcatggct atgtgaaagc aatatggttc
    10081 ctgtaaatgt ttacatcact acaggcatca gcgaacacaa caatgaattg ctttcaacgt
    10141 atccgaatcc tgtttcagac caacttcata tttacaatcc attcggcaca actaaagcta
    10201 cacttcttga tattacagct cgtgttgtta atacttacat gctgaataat ggtgaaaaca
    10261 gtttcgacat tgcatcattg aatgcaggag tttatcacat tcaggtagaa aatgaaggag
    10321 taattactaa cttaaagttt gtaaagaatt agcaaccact gaaagtgctt ttacactaat
    10381 aaagcaaagc actttgatta aagaataaaa gcttcataaa caaaaaacag cagccggcaa
    10441 aagtgggttg ctgttttttg cttagtaggt cttactattg ttgctaattt ggaatttaag
    10501 ccatgtcata aattatccaa cggacttttg atttttcgtc tggtttgatc ggtaaggctt
    10561 gtataaatca ttgttgtttt gatattatta tgtcctaata gatcttgcac aaaacgtatg
    10621 tcagtaccct gctctataag atgtgtagca aaactgtgac gcaaaccatg tacacctatt
    10681 tttttgtaaa tctttgcttt tctcatagtg ttcttaaaca taagttgtac acttctgata
    10741 ttatattgcc cgccatcctg cccttcaaac aaatattttg ttggcttata ttctttataa
    10801 taggctcgta atagttccaa caccgaattg gggagtattg catagcgatc cttcttgccc
    10861 ttcccgcggc ttatcaacac ctgcattcgc ttgctgtcaa tatctgcaat tttcagatta
    10921 acaatttcgc tcacacgcaa acccattcca taacacaatt taagaattag cagatgcttt
    10981 ttgttttcaa ctgctgaaaa catcttcctg atatctccgg ctgacagtgt tttagggagg
    11041 gtgctcggtt tctttggtct tggaacttct ataaatactt tctttcgctt aagtacttgc
    11101 tcatagtaaa acttaatagc attcattcgg ctgtgtatgg tgttttctga cagctttaat
    11161 gttttaatac aatacaagaa gtatgaacgt agtttttcgg aacttaaact ttcagccttt
    11221 gttgttttta ttactttaag cagttgtaca aactcagtag aataggtacg catagtattt
    11281 gggctgtagc ctttaagctg aagttgttct acaaaacggt taaaagcaga atgattaaca
    11341 gcattcacat cagctaaaac atgactgctc ataaccattg cttctatgcc aaataatttt
    11401 cgataacttg ttttgtccaa aacataccat tttttattgc ttctgctcca ctgcgctgac
    11461 gtgtgttgtt taagatgctg gatcagtttg gtgttgtatg gaaaagcaat ccatatcacc
    11521 tttttatttt ggtgtgtgcc aatttcaaaa ttgtagcatt gcggttcaaa gggcttataa
    11581 gcatccatct ttaattcgta ttttgtattt atgactgaaa tatacgaaat agtttctgga
    11641 atatgcaata caaaaagcga atagaatgtt ctttaattaa aaagctgtat atcaacatgt
    11701 ttaattttta tattgataaa cttaccttct tgaggaaact tagaaaagat tcgtatattt
    11761 gtgagttatg ggcaactcta tgagacaacc acgaaacgga cgggaaccac cagacgactg
    11821 actttaaaag ttcagttgac aagccaatgg acaactttaa aaccattgca caaaaacaat
    11881 tttcaaactt taaaatcaaa ta
//
LOCUS       DAOQNP010000087        11317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2038_length_11317_cov_8.175191, whole genome shotgun sequence.
ACCESSION   DAOQNP010000087 DAOQNP010000000
VERSION     DAOQNP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2038_length_11317_cov_8.175191"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3190
                     /locus_tag="PKY09_10715"
     CDS             <1..3190
                     /locus_tag="PKY09_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB13368.1"
                     /translation="SVLTPTHTYTSSGNYQVALTVNSSGGCVDSLIQQITIYPKPVAQ
                     FTAPAVCLNQQTNFNNQSTVNGNNINAWSWNFGNGNVSNVQSPAITYMSPGVYNVQLI
                     VQTAYLCSDTIVKPITIHALPIADAGVNQSVCLGNQVVLTASGGSTYNWTPVVSNSNV
                     LTITPLTTQAYYVHVTDSNNCAATDSVVVTVLLPPVADAGVDKSICKGSSTTLTATGG
                     GSYIWMPTSQTSSAVTVSPANNTMYAVVVTAPNGCTATDSVLVTVNPLPMASAGPNRN
                     ICENQVISFSASGGVSYLWYPTGDTTATIMVNPATTSAYIVEVTDINGCKAMDTMIVT
                     VFPRPIVNLTDTFVCIGYNATLDAGNAGSIFQWIPNGETTQTIQVSTAGSYGVIVTAP
                     NGCTTYDDAIVTVSDSLINSAVNVAICSGQTTTLNAGNAGSTYLWSTGATTASITVAS
                     QGTYTVTVTGTDGCSAIFNNQVNVNPLPLLQFTASPLCFGSPTQFVNQSSITSGNINS
                     FYWSFGDGMTDTSANTSYQYPLAGTYAVSLIATSTSGCVDSLTQQVTVHDIPNAKFAA
                     NNVCKNIQTTFNDQSSVANGIIVAWDWDFGDGASSQSKNPLHTYASNGIYPVDLRVTS
                     NHGCSDLYTDTVEVFALPQPQIALTSNCINIPVSMIDVSDTSGISTTNWQWNFGDGNF
                     STSQSPVHSYANAGNYMVTLQTTNSNGCIFSDTTYIDAYPNPVASFTGGPGCEGTPVT
                     FNNSSTISSGSINGFQWLFGDSSSSQATNPEHTFTPAGNYVVTLIATSDQGCIDSTQA
                     TVVIHQNPVAQFQYANHAGCGPLPVQFYDYSQSPDGSIVNWDWSFGDGNADSVQNPLN
                     IYVQTGVYNVVLTVTSQYGCVSADTAYNAVTVYPNPIAGFTPDPHETNILNPVINFIN
                     QSIGAISWDWTFGDGHSSDIYSPTHAYGDTGWYNVSQIVVNSFGCRDSAFDKVYIAPI
                     TTFYIPNAFTPNNDGTNEEFDIKGINILDYTLLINDRWGELIFEGDNKGWDGRVKGKS
                     TAAKQDVYVYTVVAKDVFGKYHKLVGHVTLLR"
     gene            complement(3191..5089)
                     /locus_tag="PKY09_10720"
     CDS             complement(3191..5089)
                     /locus_tag="PKY09_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HNB13369.1"
                     /translation="MAYKSLAHFIQVLEQSGELVRIKEYVNPHLEITEITDRISKQYG
                     PALLFENTGYYFPLLINSMGTEKRMAMALGVENLDDVAKEIEQLFKNMTAPKSSFIDK
                     LKMLPQLSTIASWMPKVISGRGECQQVVMNDIDITKFPVLKCWPEDGGPFLTLPVIQT
                     KDLHTGIRNVGLYRMQVFGKDLTAMHWHRHKVSARHFNEYKKANKKMPVVVTLGGDPA
                     YSYCATAPLPDGVDEFLLAGFIRKKSVSLVQCLTQDMQVPADADIVIEGYIDPSEEMI
                     LEGPFGDHTGYYSLADYYPKFHITCITHKKDAIYPATIVGIPPQEDAWIGKATERIFL
                     APIKMTMVPEIVDMVLPVEGVFHNLVIVKIKKDYPGQALKVMNALWGAGQMMFTKMMI
                     VVDGDVNIHNNEEVAKYISENTNPSTDFTFSSGPTDVLDHSCSTMAFGGKLGIDATKK
                     FAEELFNTKTFNSATINIPGIDEVKTILPEINGLNASLIDKNISVVFVSIEKNRKNHV
                     REAATSLMKIPFFTSVKTIIFLEHTLNIHDIADSVWRFSNNVDPKRDHFFIEHINGTT
                     IFFDGTRKTKAFDGFQRDWPNILASKTATIERIDAIWDKLGLGKFIPSPSLKYAQQLY
                     KGGAVAEE"
     gene            5207..6526
                     /gene="rmuC"
                     /locus_tag="PKY09_10725"
     CDS             5207..6526
                     /gene="rmuC"
                     /locus_tag="PKY09_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNB13370.1"
                     /translation="MTVYLILTAVITAVVVYFIVQQKLSAEIRNIKQLLSEKEIAVGS
                     MEVSLREAKSRILEAENMFSQERDAMLKLTAEHATLNTKNVDLQGRLNEQKTELEQLQ
                     QRFKTEFENLANKILEEKSVKFTEQNRINLDLVLNPLKEKIKDFEDKVEKTYKTESSE
                     RISLKEQIKNLLDLNKQLSSEANNLATALKGDNKMQGNWGEMVLEKILESSGLIKEHE
                     YKTQVVTTNVEGDKIKPDVMVFLPEQKHLIIDSKVSLLAYSELVATADETQRAKATQL
                     HVQSVKNHIKLLSDKNYQTASGYNTPDFVLLFMPIEPAFSIVMQADPEIYQFAWDKKI
                     VIVSPTTLLATLRTVASIWKQERQVKNALLIAEEGGKMYDKLVLFINDMISIGKKMDG
                     AKEDYVEAMKKLTDGSGNLIGKAQKMKELGAKAQKNLPQSLIERAGE"
     gene            6519..7769
                     /locus_tag="PKY09_10730"
     CDS             6519..7769
                     /locus_tag="PKY09_10730"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNB13371.1"
                     /translation="MNKVGAIILLFLVSCSGLHRVSNNDLSWIYKNSKLQQPKYLLYN
                     HSNDSSTIFLKLPVTAINYKQEDKSDKSIELTLKIIVFQAYESKKINDSISYSFTVPY
                     TDDTSAFILKSFYIKKPTNSSLVALRLLGDINNLFQESYIETDSSLQGINSFLFYNES
                     TDYPSFSNFISNGSTTKIQNANVQLPLWVRCFYRNYPIAEPPFSSKPPLVFKYASDSS
                     FQLVSDADKFYNLNINRYGMYQLQYDTLLKSGAALFNFGGEFPAVTTIEPMVSALRYI
                     TTLNEFNKIQQEDDKKEAVDQFWIDVSGSKERARFLIKIFYSRMQNANRFFTSYLEGW
                     KTDRGMIYLIFGSPKTVYRTSDTEYWDYGFFKGYGSLNFSFRKMANPFTNNDYVLVRS
                     AAYEPVWYMAVDHWRQGRIIQNNE"
     gene            7762..8499
                     /gene="rlmB"
                     /locus_tag="PKY09_10735"
     CDS             7762..8499
                     /gene="rlmB"
                     /locus_tag="PKY09_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNB13372.1"
                     /translation="MSNEELIYGTRAVMEAVNAGKDIEKIFIQKGVNNSLVNELRQLL
                     AKNNIPFQVVPDFKLNKLTKGNHQGVVCVLSSITYHKTEDIIPMIFERGEVPLFLMID
                     RVTDVRNVGAMARTAACAGVHAIIIPDQGSAQLNADAIKTSAGALHNILVCRENNLKL
                     TIEYLKGSGIKIVAATEKASQPYFTSDLKVPCCIIMGSEDSGISPEYLKRADEKVLIP
                     MNNVVQSLNVSVAAGIILFEAYRQRNN"
     gene            8647..11016
                     /locus_tag="PKY09_10740"
     CDS             8647..11016
                     /locus_tag="PKY09_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNB13373.1"
                     /translation="MDFQATNKKNYPSEIIEIKRTGNVFLIETANCSLEVQVYSSRII
                     RFRYGIDYLSRDFSYAKAPDFNFTDTPVELIEDDKQLVIKTSEVVCHIERDTMSISLF
                     TIDGRLINCDDGGFHWEPNPGYGGHYVYCTKQIQQHENFFGLGDKATSQNLRGKRFLN
                     WGTDTYGFAKEQDPLYRNIPFYFGLHHNISYGIFFDNSFQTFFDFGKESNTHASFWAE
                     GGQMCYYFIYAPTLMQVAEEYCKITGLPELPPKWTLGYHQSKWSYYPETVVKELAGNF
                     RSKAIPCDVIHLDIDYMKGFRCFTWDLERFPDPKRMIAELREQGFKIIVIIDPGIKKD
                     KDYWVFQQALERDYFCRRQDGDYMEGKVWPGDCYFPDFTHPDVRKWWSGLYKELLEQG
                     VSGVWNDMNEPAVFEIATFPDDVRHNYDGLNVSHRMGHNVYGMQMARATYEGLKQFTP
                     EKRPFVLTRSGFSGVQRYAAVWTGDNVASWEHIWVANMQCQRLSISGISFCGTDIGGF
                     IGEPDGELFVRYIQMAIFHPFFRGHSSSDQGDKEPWAFGAAYEALIRKSIELRYQLIP
                     YIYTTFWQYVNKGTPMIRPLAFLAQDDVETHMRMDEFGFGDQMLICPINVSASSGRAV
                     YLPKGNWYYYWNDKQFKGGHEYHVEAPLDRFPLFVKEGAVIPHQPVMQNLDGSPARQI
                     TLHVYYSSNRNISYFYEDDGENYSYRQGNFSVKKFTVFGNRRQMRIICEKFGSYKQQY
                     TECRMVIHGLPFRPHQLMIDDKEISLPRRLRDKTLTINVAANFSRIKIS"
     gene            complement(11126..>11317)
                     /locus_tag="PKY09_10745"
     CDS             complement(11126..>11317)
                     /locus_tag="PKY09_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13374.1"
                     /translation="DDFLNEANLVLSIYDNTGKLIQQKTLEMNGGNIKLNLEQEAKGV
                     YNVVLSNKKKSYNGKIVFE"
BASE COUNT         3587 a         2022 c         2207 g         3501 t
ORIGIN      
        1 ttccgtctta acaccaactc atacatacac ttcttcggga aattatcagg ttgcactgac
       61 agtaaactct tcaggtggat gtgtggatag tctgattcaa caaataacaa tatatccaaa
      121 acctgtagca cagttcactg caccggctgt ttgtctgaat cagcaaacta attttaacaa
      181 tcagtcaaca gtcaacggaa ataatatcaa cgcatggagt tggaactttg gaaacggaaa
      241 tgtttctaat gttcaaagtc ctgcaattac ttatatgtca ccgggagttt ataatgttca
      301 gttaattgtt cagacagcat atctgtgttc tgacactatt gttaaaccaa ttactattca
      361 tgcattacct attgctgatg caggtgtcaa ccaatctgtt tgtttaggta atcaagttgt
      421 acttactgca agtggagggt caacctataa ttggacacct gttgtgtcaa attcaaatgt
      481 tttaacaatt acacctttaa caacacaagc ctattatgtg catgttactg acagcaacaa
      541 ttgtgctgca acagacagtg tagttgtaac agtattatta ccaccagttg cagatgcagg
      601 ggttgataag agtatttgta aaggtagttc aacaacacta acagcaaccg gaggtggcag
      661 ttatatatgg atgccaactt cgcaaacatc gtcagcggtg acagtatcac cggcaaataa
      721 tactatgtac gctgtagtgg taacagcacc taatggatgt acggctaccg attcagtctt
      781 agtaacagta aatcctttac ctatggcaag tgcaggccca aacagaaata tttgtgagaa
      841 tcaggttata tctttttcag caagtggtgg agtttcttac ctctggtatc cgacaggcga
      901 cactactgcc accattatgg ttaatccggc aactacttct gcctatattg ttgaagtgac
      961 tgatattaac ggttgtaaag caatggacac aatgattgtt accgtattcc ctcgccccat
     1021 cgtgaattta actgatactt ttgtttgtat tggctacaat gcaacattag atgcaggcaa
     1081 tgcaggatca atctttcaat ggattccaaa tggtgaaaca acacaaacta ttcaggtttc
     1141 aacggctgga agttatggcg taattgtaac tgctcctaac ggttgtacta cttatgatga
     1201 tgcaattgtt acagtaagcg actcactgat aaatagtgca gtgaatgttg ccatttgttc
     1261 aggtcagaca actacactga atgcaggaaa tgccggctca acttatcttt ggtcaacagg
     1321 tgctacaact gcatccatta cagttgcttc acaggggaca tacacagtga ctgtgacagg
     1381 aacagatgga tgctctgcaa tctttaacaa tcaggttaat gttaacccgt tgccattact
     1441 gcaatttaca gcatcaccat tatgtttcgg aagcccaaca cagtttgtaa atcagtcaag
     1501 cattacttcg ggaaatatca acagtttcta ttggagcttt ggtgatggta tgactgatac
     1561 ttctgctaat acctcttatc aatatccgct tgcaggaaca tacgctgtat cgctgatagc
     1621 aacttcaact tcaggatgtg tggattcatt aacacaacaa gtcactgtac atgacattcc
     1681 taatgcaaag tttgcagcta acaatgtttg taaaaacatt cagacaacct ttaatgatca
     1741 gtcatcggtg gctaatggta ttatcgttgc atgggattgg gattttggtg atggcgcttc
     1801 ttcacagagt aaaaatccat tacatacgta tgcttcaaac ggaatctatc ctgttgacct
     1861 gagggttact tctaatcatg gttgtagtga tctatatact gatactgtag aagtatttgc
     1921 attgccacaa cctcaaatag cattgacaag taactgtatt aacattcctg ttagtatgat
     1981 tgatgtatct gatacttcag gcatttcaac tacaaactgg caatggaatt ttggtgacgg
     2041 aaacttttct acaagccaat cacccgtgca tagttatgca aatgctggca attacatggt
     2101 gaccctgcaa actacaaata gcaatggctg tatctttagt gatactactt atatagatgc
     2161 ttatccaaat cctgttgctt cttttacagg tggtcctggt tgtgaaggta ctccggtaac
     2221 gtttaataat tcaagtacaa tttcatcagg aagtataaac ggattccaat ggttgtttgg
     2281 ggacagtagt tcaagtcagg caacaaatcc ggaacatact tttacaccgg caggtaatta
     2341 tgttgtgaca ttaattgcta cttctgatca gggttgtatt gattctacac aggctacagt
     2401 tgtgattcac cagaatcctg ttgctcagtt tcaatacgct aatcatgcag gatgtgggcc
     2461 tttaccagtt cagttttatg attactcaca gtcaccagat ggaagtatag taaactggga
     2521 ttggagtttt ggtgatggca atgctgactc agttcagaat ccattgaaca tttatgtgca
     2581 aaccggagtt tataatgttg tgctcactgt tacatcacaa tatggttgtg ttagcgctga
     2641 tactgcttac aacgcagtaa cagtttatcc taatcctata gcaggcttta cacccgatcc
     2701 acacgaaaca aatattttaa atcctgtgat taattttatc aatcaaagca taggcgcaat
     2761 ttcatgggat tggacttttg gtgatggaca ttcttccgat atttattcac caacacatgc
     2821 ttatggtgat acaggatggt ataatgtaag tcagattgta gttaactcat tcggttgtcg
     2881 cgattctgct tttgacaaag tatatattgc gccaattaca accttttata ttccaaatgc
     2941 atttacgcca aataatgatg gcacaaatga agagtttgat attaaaggaa taaatattct
     3001 tgattacaca ctgttaatca atgatcgttg gggtgagtta atttttgaag gtgataataa
     3061 aggttgggat ggcagagtaa aaggtaaatc aacagcagct aagcaagatg tttatgtcta
     3121 tactgtagtg gctaaagatg tgtttggcaa ataccataaa ttggttgggc atgtaacctt
     3181 gttacgttaa ttattcttct gctacagcac cacctttata aagctgttgt gcatatttaa
     3241 gagaaggaga aggaataaat ttccctaatc caagtttatc ccagattgca tctattcttt
     3301 caattgtagc tgttttagag gcaagaatat ttggccagtc acgctggaat ccatcgaagg
     3361 cctttgtttt tcttgtgcca tcaaaaaaga ttgttgtacc gtttatatgt tcaataaaga
     3421 agtggtctct tttagggtca acattattgg aaaaacgcca tacactgtct gcaatatcat
     3481 ggatgttaag tgtatgttcc agaaaaataa ttgttttcac tgaggtgaaa aatggtattt
     3541 tcataagaga agtagccgcc tctctgacat gattctttct gtttttttct attgatacaa
     3601 atacaacaga aatgtttttg tcaattaatg atgcgtttaa accatttatc tcaggtaaaa
     3661 ttgtttttac ttcgtcaatg ccgggtatgt taattgtagc agaattaaat gtctttgtat
     3721 taaataattc ctccgcaaat tttttagtag catcaatacc taatttgcct ccaaaagcca
     3781 tagtggagca ggagtggtct aaaacatctg tcggccctga gctgaacgtg aaatcagttg
     3841 atggattcgt gttttctgaa atgtattttg ccacttcctc gttgttgtga atgttaacat
     3901 ctccatcaac tacaatcatc attttagtaa acatcatttg tcctgctccc cataatgcat
     3961 tcattacttt cagcgcttgt ccgggataat ctttttttat tttcacaata accagattat
     4021 gaaatacgcc ttcaacagga agtaccatat caacaatttc gggaaccata gtcattttaa
     4081 tcggtgcaag aaatattctc tctgtggctt ttccaatcca ggcatcttcc tgtggtggaa
     4141 tacctactat tgttgcagga tagatagcat cttttttatg tgtaatacag gtaatatgaa
     4201 acttaggata ataatctgcc aatgaataat aaccagtatg atctccaaac ggcccttcta
     4261 aaatcatctc ttcagatggg tcaatatatc cttcaattac aatatctgca tcagcaggaa
     4321 cctgcatgtc ctgagtcagg cattgaacta atgaaacact tttttttctg ataaatccag
     4381 ccagtaaaaa ctcatcaaca ccatcaggca atggtgctgt agcacagtat gaatatgccg
     4441 ggtcgccacc aagtgtaact actacgggca tttttttatt ggccttttta tactcattaa
     4501 aatgtctggc actaacttta tgcctgtgcc agtgcatggc agttaagtcc tttccaaaca
     4561 cctgcattct gtataaacca acattacgaa tgcctgtatg caaatctttt gtttgtatca
     4621 caggaagagt aagaaacggg ccaccatctt caggccagca ttttagcaca ggaaattttg
     4681 ttatatcaat atcgttcatc acaacctgtt gacattctcc acgaccagaa ataacttttg
     4741 gcatccacga agctatagtg cttaactgtg gtaacatttt caatttatca ataaaactac
     4801 ttttgggagc agtcatattt ttaaatagtt gttcaatttc ttttgctaca tcatcgagat
     4861 tttctacacc taatgccata gccattctct tttcagtgcc catgctgttg atgagcaatg
     4921 gaaaataata acctgtattt tcaaataata atgcaggtcc atattgtttt gaaatacgat
     4981 cggtgatttc tgttatttcc agatgcgggt taacatattc ctttattcgt accaattcgc
     5041 cactttgttc cagaacctga ataaaatgtg ctaaactttt atatgccatg aaaaacaatg
     5101 tataatgaag tgcaaaatta gcaacattga atgatttaaa cagttattga aaacccctca
     5161 ttataatgtt tgatagaata gcttaatttt gcatgaatat cacactatga cagtttattt
     5221 aattcttact gctgtaatca ctgcagtagt cgtttacttt attgttcagc aaaaactgag
     5281 cgcagaaatc agaaacatta aacaattgct ttctgaaaag gagattgctg ttggcagtat
     5341 ggaagtttct ttgcgtgaag caaaatcaag aattcttgaa gctgaaaata tgttttcgca
     5401 ggaacgtgat gcaatgttga agttgacggc agagcatgca actttaaata caaaaaacgt
     5461 tgatctgcag ggaagactaa atgaacaaaa gactgagctg gaacaactgc aacaacgttt
     5521 taaaacagag tttgaaaatt tggccaataa aatcctcgaa gaaaaaagtg ttaagtttac
     5581 tgagcagaac agaatcaatc ttgacttagt attgaacccc ttgaaagaaa agattaaaga
     5641 ttttgaagac aaggttgaga agacttataa aacagaatct tcggagcgta tctcgctaaa
     5701 agagcagata aagaatttac ttgatctgaa taaacaactt agcagtgagg caaacaatct
     5761 tgctacagca cttaaaggtg ataataaaat gcaaggtaat tggggtgaaa tggttctgga
     5821 aaaaatcttg gaatcgtcag gattgataaa ggaacatgag tataaaacac aggtagtcac
     5881 caccaatgta gagggtgata aaattaaacc tgatgtgatg gtttttcttc cggagcagaa
     5941 acatctgatt atagattcaa aagtttcgtt acttgcctat agcgaactgg tagcgactgc
     6001 tgatgaaact cagagagcaa aagcaactca actgcatgta cagtctgtaa agaatcatat
     6061 aaagctactg agtgataaaa actatcagac agcatccggc tacaatacac ctgactttgt
     6121 gttgttattt atgcctattg agccggcatt tagtatagtc atgcaagccg accctgaaat
     6181 ttatcagttt gcatgggata aaaagattgt gattgtcagt cccaccacat tattggcgac
     6241 cctacgaaca gttgcaagta tctggaaaca ggaacgtcag gttaaaaatg cattactcat
     6301 tgccgaagaa ggcggaaaaa tgtatgataa actagtgctg tttattaatg atatgatttc
     6361 gatcggaaag aaaatggacg gagctaaaga agactatgtt gaagcaatga aaaagttgac
     6421 cgatggatcg ggaaatctta ttggcaaagc acaaaagatg aaagaacttg gagctaaggc
     6481 gcagaaaaac ttacctcagt cattaattga aagagcaggt gaataaagtt ggagcaatca
     6541 tacttctttt tttagtgtca tgcagtgggc ttcaccgcgt aagcaacaac gacctgtcat
     6601 ggatttataa aaacagcaaa ttacaacaac cgaaatatct tttatacaat cattcgaatg
     6661 acagcagcac aatttttttg aaactacctg ttacagcaat taattataag caagaagata
     6721 agagtgataa aagcattgaa ctaacactga agattattgt ttttcaagct tatgaaagta
     6781 aaaagataaa cgatagtatt tcttattctt ttaccgttcc ctacactgat gatacttcag
     6841 catttattct caaatcattt tacataaaga aaccaaccaa cagttcattg gttgcattac
     6901 gtttactagg tgacatcaac aatctttttc aggaaagtta tattgaaacc gacagcagtt
     6961 tgcaggggat taattctttt ttgttttata atgaatcaac tgactatcca agtttcagta
     7021 actttatttc aaacggtagt acaacaaaaa ttcaaaatgc taatgtccaa ttgccattgt
     7081 gggtaagatg tttttacaga aattatccta tagcagaacc accattcagc agtaaacctc
     7141 cattagtttt caagtatgca tcagacagtt cgtttcagtt ggtgagtgat gcagataagt
     7201 tttataacct gaacataaac cgctatggta tgtatcagtt gcaatatgat accttgctca
     7261 aatcaggtgc tgcattattt aattttggag gtgagtttcc ggcagtaacc actattgaac
     7321 ccatggtatc ggctttacga tacattacca cactcaatga gtttaacaaa attcaacaag
     7381 aagatgataa aaaagaagct gttgatcaat tctggattga tgtttcagga agcaaggaga
     7441 gagcaaggtt tctgataaaa atattttatt caagaatgca aaatgccaat cgttttttta
     7501 cttcatatct cgaaggatgg aaaaccgaca ggggaatgat ttatttaatt tttggttcac
     7561 ccaaaactgt ttatcgtacc agtgatacag aatattggga ttatggtttt tttaaaggat
     7621 atggaagttt aaatttctca ttcaggaaaa tggctaaccc attcacaaat aatgattatg
     7681 tacttgtccg ttcagcagct tatgagcctg tttggtatat ggctgttgac cactggcgac
     7741 aaggtagaat aatacagaac aatgagtaat gaagaattaa tatacggcac acgcgctgtt
     7801 atggaagcag tgaatgcagg aaaggatatt gaaaaaatat ttatccaaaa aggtgtcaat
     7861 aacagcttgg taaatgagct aagacaattg cttgccaaga ataatattcc gtttcaggtg
     7921 gtgcccgatt ttaaactaaa caagcttaca aaaggaaatc atcagggtgt tgtttgtgtt
     7981 ttgtcatcaa taacctatca taaaacagag gatattattc caatgatttt tgagagaggc
     8041 gaagtgccac tttttttaat gatagatcgt gtaacagatg tacgtaatgt tggcgctatg
     8101 gcacgcacag cagcctgtgc aggtgttcat gcgattataa ttccggatca aggctcagcg
     8161 caactgaatg ccgatgctat taaaacttct gcaggtgcat tgcacaacat tcttgtttgc
     8221 cgtgaaaata atcttaaact taccattgag tatctgaagg gctcaggaat taaaattgta
     8281 gcagcaacag aaaaagcaag ccagccttat tttacatcag atttgaaagt gccttgttgc
     8341 atcatcatgg gtagtgaaga tagcggcatt tcgcctgaat atctgaagcg tgctgacgaa
     8401 aaagtattga tacctatgaa taatgtggtt caatcgttaa atgtttctgt tgcagccggc
     8461 atcatccttt ttgaagcata tagacagcga aacaattgaa atacagctat cttaatcttt
     8521 tagtttcatc aacaatttta acttaaaaac tcctttagtg atgtgcggaa aacatatccg
     8581 taaattgttt tatttgcaac aatagaagat ggtggaataa attaattccc gacactaaat
     8641 ttttttatgg attttcaagc aacaaataag aagaattatc cgtctgaaat tattgagata
     8701 aaacggacag gtaatgtatt tttaatagaa actgccaatt gtagtttaga agtacaggtt
     8761 tattcatcaa ggataattcg ttttcgttat ggcatagatt atttatcacg tgatttttcc
     8821 tacgccaaag caccagattt taatttcact gatacacctg ttgaactgat tgaagatgat
     8881 aagcagttag tgattaaaac ttctgaagtt gtttgccaca ttgaacgtga caccatgagt
     8941 atttcactct ttactattga tgggcgattg atcaattgtg acgatggcgg atttcattgg
     9001 gagcctaatc ccggctatgg ggggcattat gtttattgca caaaacaaat tcaacagcat
     9061 gagaattttt tcggacttgg tgacaaagca acttctcaga atttaagagg gaaaagattt
     9121 ttaaactggg gtactgacac ctatggattc gccaaagagc aagatccatt gtacagaaac
     9181 attccttttt atttcggatt acatcacaac atttcatacg gcatattttt cgataacagc
     9241 tttcagacat tttttgattt cggaaaagaa agtaacacac atgctagttt ctgggcagaa
     9301 ggtggtcaga tgtgttatta ctttatttat gcacctactt tgatgcaggt tgcagaagag
     9361 tattgtaaaa tcacaggact tcctgaatta cctccaaaat ggactttagg atatcatcaa
     9421 agcaagtgga gttattatcc tgaaactgta gttaaagaac tggccgggaa tttccgcagt
     9481 aaggccatcc cctgcgatgt catccatctg gatattgact acatgaaagg tttcagatgt
     9541 tttacctggg accttgaacg ttttccggat ccaaaaagga tgattgcaga attgcgtgaa
     9601 caaggtttta agattattgt aatcattgat cctgggatta aaaaagataa agactattgg
     9661 gtgtttcaac aggcacttga aagagattat ttctgtcgca gacaggacgg tgattatatg
     9721 gaaggtaaag tatggcccgg tgattgttat tttcccgatt tcacgcaccc cgatgtaaga
     9781 aagtggtgga gtggtttgta taaagaactc ttggagcagg gtgttagtgg tgtttggaat
     9841 gacatgaatg aacctgcagt ttttgaaatt gcaacatttc ctgatgatgt acgacacaat
     9901 tatgatggac tgaatgtaag ccatcgcatg ggacataatg tttatggtat gcaaatggca
     9961 cgcgccacct atgaaggact taaacaattt actcccgaaa aaagaccttt tgttttaacg
    10021 cgttcaggat tttcaggtgt gcagcgctat gcagcagtgt ggacaggaga caatgttgca
    10081 tcttgggagc atatatgggt tgccaacatg caatgtcaac gactgagtat ttccggaatt
    10141 tctttttgtg gaacagacat tggtggattt atcggtgagc ctgatggcga attgtttgtt
    10201 cgctatattc aaatggcaat tttccatcca tttttcagag gccattcaag cagcgatcag
    10261 ggtgataaag agccttgggc ttttggtgca gcttacgagg cacttatcag aaaatcaatt
    10321 gaactccgat atcagttaat cccttacata tacaccacat tctggcagta tgtaaacaag
    10381 ggaacaccca tgataagacc attagctttc cttgcacaag acgatgttga aacacacatg
    10441 cgcatggatg aatttggttt tggagatcag atgcttattt gcccaatcaa tgtaagtgca
    10501 tcaagtggca gggctgtata tctgcccaaa ggaaactggt attactattg gaatgataaa
    10561 caattcaaag ggggacatga atatcatgtt gaagcaccac ttgaccgctt tccactcttt
    10621 gttaaagaag gtgcagttat tccgcatcag cctgtaatgc aaaatctgga tggtagtcct
    10681 gcgcgtcaga ttactttaca tgtttactat tcatcaaaca gaaatatcag ctatttctat
    10741 gaggatgacg gagaaaatta ttcatacagg caaggaaatt tctcagtaaa aaaatttact
    10801 gtatttggca acagaagaca aatgagaata atttgtgaga agtttggatc ctataaacag
    10861 caatatacag agtgtaggat ggttattcat ggacttcctt ttcgcccaca tcagttgatg
    10921 attgacgata aagaaatttc tttgcccaga cgcctgcgtg acaaaacact taccatcaat
    10981 gtagcagcca acttcagcag aataaagatt agctgaggtt taatggatag acgaaagcta
    11041 ctgattacat taatactatc agcatgaatt agcagcttgc tttggagtgt ttcgggatat
    11101 gaaactatga aaatcctatt taatattact caaacacaat cttcccatta taactcttct
    11161 ttttattact cagcacaaca ttataaacgc cttttgcctc ctgctccaaa ttcaatttta
    11221 tattgccacc attcatctct aatgtttttt gctgaatgag tttgccggtg ttgtcataaa
    11281 tgcttaaaac aaggtttgct tcatttaaaa aatcatc
//
LOCUS       DAOQNP010000088        11011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2120_length_11011_cov_7.184648, whole genome shotgun sequence.
ACCESSION   DAOQNP010000088 DAOQNP010000000
VERSION     DAOQNP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_11011_cov_7.184648"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKY09_10750"
     CDS             <1..360
                     /locus_tag="PKY09_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNB13375.1"
                     /translation="AVLCWVSGFDIIYALQDEQFDRENKLHSIPSLMGKKHALQVSRI
                     LHFLTAGFLALFLYQAHIGLLAWSGLAVFCALLIYQHSLVSENNLSKVNIAFATTNGI
                     ASIVFAGMVITDIFLLN"
     gene            complement(452..2188)
                     /locus_tag="PKY09_10755"
     CDS             complement(452..2188)
                     /locus_tag="PKY09_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13376.1"
                     /translation="MIRTIQQSTKAAIILIMFSYIGNPFPAYAKGKALKATLKGQNVT
                     CFGGNNGAINLDISGGNAPYQITWNDGSSGYAMLDKPAGEYSVLITDSKGSIVTQSIT
                     LTEPYAMTIYSQVTSEQCYARNGKASLLVTGGTQPYQYEWSNQTRQQNLEAVAAGDYE
                     VKVTDKNGCVKMSNITIERNKVLELNIAKYDPLCNDEASGLIDVEVKGGWAPYTFEWS
                     DGAKTEDQSGLKAGNYSVIVKDNNGCIAKAEVQLKNPDAIKVEAKITEADLYKSNGSI
                     ILNVSGGVGNYTYLWSNMENSQSLNNIEEGVYAVRVDDSHRCSTSAYFTVNEKSPMQV
                     ESVVKHELCNGGKNGSIKLNVKGGVAPYKFEWSNEMSGATISNLSAGDYSVLITDANN
                     KVYRNTFTVKQPEPIGIFATVLDESVIGKNDGSIKLQVIGGMPPLKYQWSNGQTVSDL
                     ANLSQGTYTVRMKDGNGCAMEHHVLVGLNYTDAMLRTAYEKEDHVMMEIYPNPFSNNF
                     TMKFSTPVSNIQSIIVYAMDGKIAEQLNPDLEQQQVNEIAVNASKLKPGNYLIKVTTD
                     KKIFTRIVTRTM"
     gene            complement(2313..3482)
                     /locus_tag="PKY09_10760"
     CDS             complement(2313..3482)
                     /locus_tag="PKY09_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13377.1"
                     /translation="MLRIEVFKSVTEVPLHDWQTINGENVIFTSFSYLSALEQSKPAG
                     MQFRYVIVYDNKMPVATLCFQLLDVANKDLDGVLNLKDKGWLLQNLNKTINKLLFKCQ
                     TGKVNFLICCGSFFVSGEYGINVINRDVLSAIIQILPEVRLKIVSTINNASVCGWMVK
                     DFYNNTRIDQPLLEDGFFELPMDPEMILFIREQWQTLEDYLAALTTKYRVKANSVIRK
                     IEDVEIVALDVKDILKYGDDLIRLYLNIQNKATVQMARVNILYFEKLLTIMPEKFYVK
                     AFIKESKMIAFYCGFQHAGHHEAHFIGIDYSLNKTLSLYQNILYRFIEEAIKQRASNL
                     YYGRTAMEIKSTTGAKAYPLHAYFNLSNTVLNKMVKPVVKRFTPEKWVPRNPFRE"
     gene            3611..4900
                     /locus_tag="PKY09_10765"
     CDS             3611..4900
                     /locus_tag="PKY09_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNB13378.1"
                     /translation="MKYNSINSKLFVDNRKKFTAELKPKSIAIFQSNDEMPRSGDQNF
                     TFRQNADLFYLTGIDQEESILVIFPDSPLPQYKEILFVRKTNERIAIWEGHKYTKEEA
                     QATSGIKHIYWTEEFNAVVPMLMHHAENVYVNINENDRHATEVPYRDMRFAREWKNRF
                     PAHNFERLGQIMAKLRSRKHEIEVQLISEAIKHTESALRRVLKFMKPGVMEYEVEAEI
                     IHEFVRNRCNGHAYSPIIASGASACVLHYNENNKPCKDGDLVLMDFGAEYANYAADLT
                     RTIPVNGKFTPRQKEVYEAVLRVMKAATKLLVPGNFIEKYHAEVGKMMEEELVNLKLI
                     TKDDIKNQNPEWPAYKKYFMHGTSHFLGLDVHDVGNKYQAMQAGMVFTCEPGIYIPEE
                     SIGIRIENNILITDKGPVDLMATIPVEVSDIESLMRK"
     gene            4935..6203
                     /gene="glyA"
                     /locus_tag="PKY09_10770"
     CDS             4935..6203
                     /gene="glyA"
                     /locus_tag="PKY09_10770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNB13379.1"
                     /translation="MQKDKTVFKLIKEELARQRHGLELIASENFVSQQVMDAMGSCLT
                     NKYAEGLPGKRYYGGCEVVDEIEQIAIDRAKTLFNATWANVQPHSGAQANAAVMLACL
                     QPGDTILGFNLSHGGHLTHGSPVNFSGKLYRPTFYGVEENTGTIDYNKVEETALREKP
                     KLIICGASAYSRDWDYVALRSIADKINALLLADISHPSGLIARGLLNDPLPHCHFITT
                     TTHKTLRGPRGGLIMMGKDFENPWGLKTPKGETRMMTALMDSAVFPGTQGGPLEHVIA
                     AKAVAFGEALSDEYFEYCLQVIRNAKTMANAFVKKGYKVISGGTDNHLMLIDLRSKNI
                     TGKQAENALVRADITVNKNMVPFDTQSPFVTSGMRVGTPAMTSRGMTETEMVRIVELI
                     DTVIMNHTNDAVIEEVKTAVNDMMDHFPLY"
     gene            6272..7330
                     /gene="porQ"
                     /locus_tag="PKY09_10775"
     CDS             6272..7330
                     /gene="porQ"
                     /locus_tag="PKY09_10775"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNB13380.1"
                     /translation="MRILTLLAALLLPVCLKAQTGGSAIFRFLDLPSSARHAALGGNF
                     ISIRDNDLNTSFFNPAILNVDMDKQITFNYNPYFSGIKYGYTAYAHHIENIGTLSAGI
                     QYVSYGKFDRTDETATDLGTFSGGDYALNVSMSRPVGIDSVLFVGASLKFIYSHIDTY
                     SAFGIAADLGVNYYSPNRLTSAALVVRNAGRQLKSYTEKNNEPLPIEIEAGITQKLAK
                     APFRFTLTYRHIEKWDLSYIDNNSQETDPLTGEIKEPEKASFGNKLLRHLIISNEILI
                     SKNFHLRMAYNFQRRNEMKVESRPAMVGISFGLGLKISKFHLSYGRSLYHLAGGTNSL
                     SISTYLNDFKRKPKATES"
     gene            7406..8104
                     /gene="cmk"
                     /locus_tag="PKY09_10780"
     CDS             7406..8104
                     /gene="cmk"
                     /locus_tag="PKY09_10780"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNB13381.1"
                     /translation="MIIAIDGYSSCGKSTVAKAIAKRLKLNYIDSGAMYRAVTYYFLQ
                     NNIPFSKTADDVATEYTSILENIHIQFIRNEQTGQSEIYLNGKNIEVEIRQMDVSDNV
                     SYVSAIKSVRKKLVVMQQQMATMGHLVMDGRDIGTTVFPNADLKIFMTASPEVRVYRR
                     YHELIEKGIKVTPDEIRKNIELRDYEDTHREESPLRKPDDAIILDNTYMSRDEQVQFV
                     LDAVAKLKKAIKSG"
     gene            complement(8112..9911)
                     /gene="argS"
                     /locus_tag="PKY09_10785"
     CDS             complement(8112..9911)
                     /gene="argS"
                     /locus_tag="PKY09_10785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNB13382.1"
                     /translation="MIFEKELASITNEALKALFDFEPDESLLTYTQTRKEFDGDYTIV
                     VFPFVRPSKKSPEETGRLIGEYLIKHASFISNYNVIKGFLNISISDEQWLKKVAVAFA
                     NPDYGFSKPDKSKPVIVEYASPNTNKPIHLGHLRNILLGYSVSLLLKATGRNIKRVSI
                     INDRGVHICKSMLAWKKFGNGETPQSSGLKGDHLVGKYYVEFDKHYKQQVSELIAAGI
                     EKDKAEKSAPLMQEVRQMLIEWELGNEEVRALWKMMNSWVYEGFDVTYNRLGVDFDKT
                     YYESETYLLGKKIVEEGLSKGVFYKKADNSVWVDLTSDGLDEKIVQRSDGTSVYITQD
                     LGTALLRHDEFDFDSKIYVVGNEQDYHFKVLFLILKKLGYAWANKLYHLSYAMVDLPS
                     GKMKSREGTVVDADDLMDEVTAAAAEETSKLGKIEEFSSDELKQLYETLGFGALKYFI
                     LKVDPEKRMLFNPAESVEIHGNTGPYIQYVHARICSVLKRASQMAQLNDYRNMALESV
                     VSMNDKERELAINILRFKETVETAAEKLSPAIVANYVYELAKSFNQFYHDNSIISEGE
                     LETSRFRLCLADACALTIKKSLNLLGIKAPERM"
     gene            complement(9923..10804)
                     /locus_tag="PKY09_10790"
     CDS             complement(9923..10804)
                     /locus_tag="PKY09_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNB13383.1"
                     /translation="MTIIDGKQTAEEYKALLKQEVNVLVATGKRPPGLAILLIGNNGA
                     SETYVANKLKTCHDVGFNASLFRFENTVSENEILNCINELNFNSSIDGVIVQSPFPNH
                     ISPQKINEAIHPAKDVDGFHPMNAGRLQQNKPCYIAATPKGIMQLIEKYKIETQGKHC
                     VVVGRSNIVGMPMSILLSRNSKPGNCTVTICHSKTVDLASYTKRADILIAAIGSPELI
                     TAAMVKEGAVVIDVGITRVADKATKSGYKLKGDIKYDEVAAKCSFITPVPGGVGPMTI
                     IGLLQNTLLATKNAIYN"
     gene            complement(10835..>11011)
                     /locus_tag="PKY09_10795"
     CDS             complement(10835..>11011)
                     /locus_tag="PKY09_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNB13384.1"
                     /translation="NPDLINGNRKKRIAAGSGTGIPEVNKLIKQFEDMRKMMKMMTTG
                     NMKNMMRNMPGMRR"
BASE COUNT         3436 a         2209 c         1878 g         3488 t
ORIGIN      
        1 gctgttcttt gctgggttag tggattcgat attatttatg ctttgcagga tgagcaattt
       61 gatcgtgaaa ataaattaca ctccattcct tcacttatgg gaaaaaaaca tgcattgcag
      121 gtatcaagga ttttacattt tttaacagca ggatttcttg ctctttttct ttatcaggct
      181 catattggct tgttagcgtg gtcagggttg gcagtatttt gtgcactgct tatttaccag
      241 cacagtttgg tatctgaaaa taatcttagc aaagtaaaca tagcctttgc cactacaaat
      301 ggcattgcca gcatagtttt tgccggcatg gttattacag atattttttt attgaattaa
      361 ttattacctg ccaactgtta taaacggtta caggcgcaga gaaccaccaa ctgcgcctgt
      421 gaaaaacaac caaccatgaa aagtattgat actacattgt tcgtgtaacg attctcgtaa
      481 agattttttt gtctgtggtt acttttatta aataatttcc gggtttcaat tttgaagcat
      541 tgactgcaat ttcattaacc tgctgttgtt ctaaatcagg attcagttgt tcagcaattt
      601 ttccatccat ggcataaaca ataatgcttt gtatgttgct gacaggcgta ctgaatttca
      661 ttgtaaaatt gttgctgaat ggattgggat aaatttccat cataacatgg tcttctttct
      721 cataagcagt tctcagcatg gcatctgtgt aatttaatcc aaccagcaca tgatgttcca
      781 tagcacaacc gttaccatct ttcatgcgca ctgtgtatgt tccctgcgat aagttagcta
      841 aatcagaaac ggtttgccca ttgctccact gatattttaa tggaggcata ccacctatta
      901 cctgcaattt aatgctgcca tcattttttc ctataacact ttcatcaagt acagttgcaa
      961 atatgccgat tggctcaggc tgcttaacgg taaaagtatt tctataaact ttattattag
     1021 catcagtaat gagcacactg taatcaccgg cagaaagatt gctgatagtt gctccactca
     1081 tttcattcga ccattcaaat ttataaggtg ctacccctcc ttttacattc agttttattg
     1141 aaccattttt tcctccatta cataattcat gtttaacaac ggattcaact tgcataggcg
     1201 atttttcatt cacagtaaaa tatgctgatg tactacaacg atgcgagtca tcaactctga
     1261 cagcataaac accttcttca atattattca acgactgact gttttccata ttcgaccata
     1321 gataagtgta attaccaaca ccacccgaaa catttaatat tatgctacca ttgcttttat
     1381 acaaatctgc ctcagtaatt tttgcttcca cttttattgc atcaggattt tttaattgca
     1441 cttcagcctt agcaatgcaa ccattattat ctttaacaat cacagaataa tttcctgctt
     1501 ttaatccgct ctgatcttct gttttagcac catcagacca ctcaaaggta tatggagccc
     1561 aaccaccttt tacttcaaca tctatcaaac cactggcctc gtcattacat aaaggatcat
     1621 atttggctat gtttaattca agaactttat ttctttcaat agtgatgttc gacattttta
     1681 cacagccatt tttatcagta acttttactt catagtcacc ggccgcaaca gcttcaaggt
     1741 tttgctgacg cgtttgattc gaccactcat actgatatgg ctgcgtgccg cctgttacca
     1801 ataagcttgc tttaccatta cgtgcataac actgttctga agtaacctgc gaataaattg
     1861 tcatggcata aggttcagtg agcgtaatag attgtgtgac tatactgccc ttagaatccg
     1921 ttattaaaac agaatactca cctgcaggct tatctaacat tgcataacct gatgatccat
     1981 cattccatgt aatctgatat ggcgcattgc cgccagaaat atcaagattg atagcgccat
     2041 tgttgccgcc aaaacaagta acgttctgac cctttaatgt tgctttaagt gctttgccct
     2101 ttgcataagc aggaaatgga ttgccaatat aactgaacat aatcagtatg atggctgctt
     2161 ttgttgattg ttgaattgtt ctaatcattg gtggttcaat taagatgctt gcaagctatc
     2221 acaccaccaa tgcagcaagg cactgtttta tgttaacatg ttaaattttg gggtgaaaat
     2281 tcaaataaaa acggcaaacg gtttttaggc aactactccc tgaaaggatt tctgggcacc
     2341 catttctccg gtgtaaatcg ctttacaacg ggcttcacca ttttattcaa aactgtatta
     2401 ctcagattaa aatatgcatg taatggatag gctttggcac cggtggttga ttttatctcc
     2461 atagcagtcc gtccatagta aagatttgac gcacgctgtt tgatggcttc ttcaataaaa
     2521 cgatataata tattctgata aagagaaagt gttttattca aactgtaatc tattccaata
     2581 aaatgtgctt catgatgacc tgcatgttga aatccacaat aaaaggcaat cattttactt
     2641 tctttaataa aagctttaac atagaatttt tccggcatga tggttaagag cttttcaaaa
     2701 tataaaatat tgactcttgc catctgcact gttgccttat tttgaatatt cagataaagt
     2761 cgaattaaat catctccata tttcaaaata tcttttacat ctagtgcaac aatctcaaca
     2821 tcttctatct ttctgattac actatttgcc tttacccgat atttggttgt cagagccgcc
     2881 aaataatcct caagagtttg ccactgttcc cgaatgaata aaatcatttc agggtccatt
     2941 ggcaattcaa aaaagccatc ttccagcaag ggttggtcta tcctagtgtt gttataaaaa
     3001 tctttcacca tccatccgca aactgatgca ttattaattg tacttacaat tttcaatctc
     3061 acttcaggca gaatttgaat tatggcagac aagacatctc tgttaataac attgatacca
     3121 tattcaccac tcacaaaaaa acttccgcaa caaattaaaa aatttacttt cccggtttga
     3181 catttaaata acagtttgtt gattgtctta ttcaggttct gcaacaacca tcctttatct
     3241 tttaaattca gtacaccatc caaatcttta ttggcaacat caagcaattg aaaacataag
     3301 gtagcaacag gcatcttatt atcgtaaaca atcacgtatc taaactgcat accggcaggc
     3361 ttgctttgtt ccaatgctga caaataagag aatgaagtaa aaatgacatt ttcaccattt
     3421 atggtctgcc agtcatgtaa cggtacttca gtcacacttt taaaaacttc tattcttaac
     3481 atattgctct tcaatacact tcttacgctg tcatttagcg acaggcacaa ttaaaacatt
     3541 taaaaaaaca aagcacgtaa aaattatttc acgttatttg taacagttaa aagtaaagtt
     3601 agttatgaat attaaataca attccatcaa cagcaagctt tttgttgaca acaggaaaaa
     3661 gtttacagca gagttaaaac ctaaatcaat tgctattttt caatctaatg atgagatgcc
     3721 gcgtagtggc gatcagaatt ttacgtttcg tcagaatgca gatttgtttt acctcacagg
     3781 tattgatcag gaagaaagta ttttagtaat atttcctgac tcaccattgc cccaatacaa
     3841 agagatttta tttgtaagaa aaaccaatga acgcattgcc atctgggagg ggcataaata
     3901 cactaaagaa gaggcacaag ccacctctgg tataaagcat atctattgga cagaagaatt
     3961 taacgctgtt gtgcccatgc tgatgcatca tgcagagaat gtttatgtca acataaacga
     4021 aaatgatcgt catgctactg aagtacctta tcgtgatatg cgctttgcac gtgaatggaa
     4081 aaaccgtttc cctgcacata attttgaacg acttggtcag ataatggcta aattgcgctc
     4141 acgcaagcat gaaattgaag ttcagttaat cagcgaagct attaaacata cagaatctgc
     4201 attaagacgt gtgttgaaat ttatgaaacc gggtgtcatg gaatacgaag ttgaagcaga
     4261 aattattcat gagtttgtac gtaatcgttg caatggtcat gcatatagtc ctataattgc
     4321 atcaggcgct tctgcatgtg ttttacatta taacgaaaat aacaaacctt gtaaagatgg
     4381 cgatctggtg ctgatggatt ttggtgccga gtatgcaaac tatgctgcag atttaacaag
     4441 aaccattcct gtgaatggaa aatttacgcc aagacagaaa gaagtatatg aggctgtttt
     4501 gagagtgatg aaagcagcaa ccaaacttct tgtcccaggc aactttattg aaaaatatca
     4561 tgccgaagta ggtaaaatga tggaggaaga attggtgaat cttaaactga taaccaaaga
     4621 tgacattaaa aatcaaaatc cggagtggcc tgcctataaa aaatatttta tgcatggcac
     4681 atctcacttc cttggacttg atgtacatga tgtaggaaac aaatatcagg ctatgcaggc
     4741 aggaatggtg tttacttgtg agcctggaat ttatattccc gaagaaagca taggaatacg
     4801 tattgaaaat aatatcctca tcaccgataa aggcccggtt gaccttatgg caacaatacc
     4861 ggttgaagta agtgacattg aatcattgat gcgtaaataa tcataaaaaa taatagaaaa
     4921 catactctac aaatatgcaa aaagacaaaa ctgtttttaa actgataaaa gaagaacttg
     4981 cccgccagcg tcacggtttg gaattgatag catcggaaaa ttttgtcagc caacaggtga
     5041 tggatgccat gggctcatgc ctgacaaaca aatatgcaga aggacttccc ggcaaacgct
     5101 attatggcgg ctgcgaagtt gttgatgaaa tagaacagat tgctattgac agagcaaaaa
     5161 cactttttaa tgcaacatgg gcaaacgttc agcctcattc cggtgcacaa gccaatgcag
     5221 ccgtaatgct tgcctgtctg caaccgggtg atacaattct tggttttaac ctttcgcatg
     5281 gtgggcactt aacgcatggt tcacctgtaa atttttcagg caaactttat cgtcctacat
     5341 tttatggcgt tgaagaaaat acaggtacaa ttgattataa caaagttgaa gaaactgctt
     5401 taagagaaaa accaaaatta attatctgcg gagcctctgc ctattcgcgc gattgggatt
     5461 atgttgcact gcgttccatt gcagataaaa taaatgcatt gttgcttgct gatatttcac
     5521 atccctccgg acttattgca cgaggattgc ttaatgaccc tttaccacac tgtcacttta
     5581 tcactaccac aacacacaag actttacgtg gtccacgtgg tggtttaatt atgatgggca
     5641 aagattttga aaacccatgg ggactgaaaa ctccaaaagg cgaaacacga atgatgactg
     5701 ccttaatgga cagtgctgtt tttccgggca cacaaggagg accattagaa cacgttatcg
     5761 cagctaaagc tgtagcattt ggcgaagcat taagcgatga gtattttgaa tattgtttgc
     5821 aggtaattcg aaatgctaaa actatggcta atgcctttgt taaaaaagga tataaagtaa
     5881 tttcaggtgg aacagacaat catttaatgt tgattgatct tcgctcaaaa aacataacag
     5941 gaaaacaagc cgaaaatgct ttagtacgtg cagatatcac cgtaaataaa aacatggtgc
     6001 catttgacac acaatctcct tttgtgactt caggaatgcg cgttggaaca cctgctatga
     6061 catcacgagg aatgacagaa acagaaatgg ttcgtatagt tgaattgatt gatactgtaa
     6121 ttatgaatca tacgaatgat gctgtaattg aagaagtaaa gactgccgtc aatgacatga
     6181 tggatcattt tccgctctat taatttattt atttcactcg ataaaaatat tttttagcat
     6241 ttagcgtttg agagtgcata aaccaaattc catgagaata ctaacgctgt tagcagcact
     6301 gttgcttcct gtttgtttga aagctcagac aggtggaagt gcaatctttc gttttcttga
     6361 tctgccatca tcagcacgtc atgcggcact cggtggaaat ttcatttcca ttcgtgataa
     6421 tgatttaaac acttcttttt tcaatcctgc catattaaat gttgatatgg ataaacaaat
     6481 cacattcaac tacaatccat atttctcggg aataaaatat ggctacactg cttatgcgca
     6541 tcacattgaa aatattggaa cactttctgc aggcatacaa tatgtaagct atggaaaatt
     6601 tgaccgcaca gatgaaacag caactgactt aggaactttt tcaggtggcg attatgcatt
     6661 aaacgtatct atgtcaaggc ctgttggaat tgatagtgtg ctgtttgtag gtgcttcatt
     6721 aaaatttatt tacagtcata tagacaccta ctctgctttt ggaattgctg ccgaccttgg
     6781 tgtcaactat tacagtccaa atagactgac aagtgcagct cttgttgtta ggaatgcagg
     6841 ccgtcagcta aagtcctata cagagaaaaa caacgagcct ttgccaatag aaattgaagc
     6901 aggcattaca caaaagttag ccaaggcgcc ttttcgattt actttaactt atcgtcatat
     6961 tgaaaaatgg gatctttcct atattgacaa caacagtcag gaaaccgatc cattgacagg
     7021 tgaaataaaa gaacctgaaa aagcatcctt tgggaataaa ttattgcgcc atctgataat
     7081 cagtaatgag attcttatca gtaaaaattt tcatttgcga atggcttaca atttccaaag
     7141 aagaaatgag atgaaagtag aaagcagacc ggcaatggtt ggtatttctt tcggacttgg
     7201 cttaaagatt tctaaattcc atctcagcta tggcagaagt ctttaccatc ttgcaggtgg
     7261 aaccaacagt ctttctattt caacttatct aaacgatttt aagcgcaagc caaaagcaac
     7321 agaaagttaa gatgaaataa agttaagatt cactgttgtt cttattgctt ttcattattt
     7381 ttgaaaagtt ttccggataa gttttatgat aattgctatt gatggttatt catcctgcgg
     7441 gaaaagtacg gttgcaaaag ccatagcaaa gcggcttaaa ttgaattata ttgattcggg
     7501 tgccatgtac cgcgcagtga cttattattt tctccaaaac aatattccat tcagcaaaac
     7561 tgctgatgac gttgcaactg agtacacctc tattcttgaa aacatacaca ttcaatttat
     7621 cagaaatgag caaaccggac aatcagaaat atatctgaat ggcaaaaaca ttgaagtaga
     7681 aatcagacag atggacgttt cagacaatgt cagttatgtg agtgcaataa aatcggtacg
     7741 aaaaaaatta gttgtcatgc aacagcaaat ggcaactatg ggacacttgg tgatggatgg
     7801 ccgtgacatt ggaactactg tttttccgaa tgccgacttg aaaattttta tgactgcatc
     7861 acccgaagta cgtgtttaca gaaggtatca tgaactcata gagaagggga taaaagtaac
     7921 acctgacgaa atcagaaaaa atattgaatt acgcgactat gaagacacac acagggaaga
     7981 aagcccgtta agaaagcctg atgatgccat cattcttgac aacacctata tgagtcgcga
     8041 tgaacaagta caatttgttt tagatgctgt tgctaagttg aaaaaggcaa ttaaatcagg
     8101 ctagcatacc tttacattcg ttcaggagcc ttaataccaa gtaagttcaa tgatttttta
     8161 attgtcaatg cgcaggcatc cgccaagcac aatctgaatc ggcttgtttc caattcacct
     8221 tccgatataa ttgaattatc gtgatagaac tgattaaaac tttttgctaa ctcataaaca
     8281 tagttagcaa caatagccgg agataatttt tctgctgcag tttcaactgt ttctttaaaa
     8341 cgaagaatat taattgcaag ctctcgctct ttatcattca tgctaacaac tgactccaat
     8401 gccatatttc tataatcatt cagttgtgcc atctgagaag cccgtttcaa aacagaacaa
     8461 attcttgcat gcacatattg aatatatggg cctgtgtttc catgtatctc tacactttca
     8521 gcaggattaa acaacattct tttttcagga tcaactttta aaataaaata tttaagtgct
     8581 ccaaagccaa gcgtttcata gagttgtttt aattcatcac tgctaaactc ttcaatcttg
     8641 cctaacttac ttgtttcttc agcggcagca gcagttactt catccatcaa atcatctgca
     8701 tcaacaacag ttccttcacg cgatttcatt ttaccactcg gcaaatcaac cattgcataa
     8761 ctcaaatgat aaagcttatt agcccatgca taccctaatt ttttcagtat taaaaacaat
     8821 actttaaaat ggtagtcctg ctcattgcca acaacataaa ttttagagtc aaaatcaaac
     8881 tcatcgtgac gcaacaatgc agtgcctaaa tcctgtgtta tataaactga agttccatca
     8941 gaacgctgta ctatcttttc gtccaagcca tcgcttgtta aatcaaccca aacactgttg
     9001 tcagcttttt tataaaacac ccctttcgac aacccttctt caactatttt cttacccagc
     9061 agatacgttt ctgactcata ataggtcttg tcaaaatcta ctcctaatct gttataggtg
     9121 acatcaaacc cttcataaac ccagctattc atcattttcc ataatgccct cacttcttca
     9181 ttgccaagct cccattctat cagcatctgt ctgacttctt gcattaaggg tgcactcttt
     9241 tcggctttat ctttctcaat acctgcagca atcaactctg aaacctgctg tttatagtgc
     9301 ttatcaaact ccacataata tttcccaacc aaatgatctc ctttcaatcc ggaggattgc
     9361 ggtgtttcac cattaccaaa ctttttccat gccagcattg atttacaaat gtgcacacct
     9421 ctgtcgttga tgatactcac acgttttata tttcgcccag ttgctttaag aagcaatgaa
     9481 accgaataac ctaaaagaat attgcgcaaa tgtccaagat gtatgggctt gtttgtattt
     9541 ggcgaagcgt attcaacaat tacgggctta gacttatccg gcttggaaaa accataatca
     9601 ggatttgcaa atgcaactgc aacttttttc agccactgct catcacttat agatatattt
     9661 aaaaatcctt ttatgacatt gtaattgctg ataaaagatg catgcttgat taaatactct
     9721 ccaattaatc ttccggtttc ttcaggtgat ttttttgacg gacgaacaaa aggaaaaaca
     9781 acaatagtat aatctccatc aaactctttg cgtgtttgtg tgtaagtcag cagactttca
     9841 tcaggctcaa aatcaaaaag cgcttttaat gcttcatttg ttatgctcgc taattccttt
     9901 tcaaaaatca tttcaaaaca gattaattat agatggcatt ttttgtcgcc agtagggtat
     9961 tctgtaacaa tccgataatt gtcatcggac caactccccc cggaaccggt gtaataaaac
    10021 tacacttagc agccacttca tcatacttaa tatcaccttt cagcttgtat ccgctttttg
    10081 ttgctttgtc tgccacacgc gtaatcccca catcaatcac tacggcacct tctttcacca
    10141 tagctgctgt tatcagctcg ggtgaaccaa tggctgcaat taatatatca gcacgttttg
    10201 tgtatgatgc aagatctact gtcttactat gacaaattgt tacggtacaa ttaccgggtt
    10261 tactgtttcg actcaataaa atactcattg gcattccaac tatattgctg cgtccaacta
    10321 caacacaatg cttcccctga gtttcaattt tatatttttc aatcaactgc ataatacctt
    10381 tgggtgtggc tgcaatgtaa caaggcttgt tctgttgtag cctccctgca ttcatcggat
    10441 gaaatccatc aacatctttt gcaggatgaa tagcttcatt tattttctgt ggtgaaatat
    10501 gatttggaaa cggtgattgt acaatgacac catcaatgct tgaattaaaa ttgagttcat
    10561 ttatacagtt caatatttcg ttttcactca ccgtattttc aaaacgaaaa agcgatgcat
    10621 taaatccaac atcatgacaa gtctttaatt tattagcaac ataagtttcg cttgctccat
    10681 tattacctat gagtaatata gctaagcccg gaggacgttt acctgttgct acaagcacat
    10741 ttacttcctg ctttagaaga gctttatact cttctgctgt ttgcttacca tcaattattg
    10801 tcatgcccaa aaaatattgt ccttatacga taaatcatct tctcattccg ggcatattgc
    10861 gcatcatatt tttcatattg ccggtcgtca tcattttcat catcttacgc atatcttcaa
    10921 actgtttaat caatttattt acttccggaa tacctgttcc gctaccggct gcaattcttt
    10981 tttttctatt tccattaatc aaatccggat t
//
LOCUS       DAOQNP010000089        10653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2228_length_10653_cov_8.922344, whole genome shotgun sequence.
ACCESSION   DAOQNP010000089 DAOQNP010000000
VERSION     DAOQNP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_10653_cov_8.922344"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..876)
                     /locus_tag="PKY09_10800"
     CDS             complement(196..876)
                     /locus_tag="PKY09_10800"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNB13385.1"
                     /translation="MSQKRFAIIVAGGSGTRMQANVPKQFLLLEGRPVLMYSIECLHR
                     VGADVTVVLPDIEHSHWQSLIQEYHFKVPHKISSGGLTRNRSVFNGLQTIDDNEALVA
                     VHDAARPILKPELAEKLFEHAAHRGNAIPVVAVRESLRRINSAENKAVNRAEIFAVQT
                     PQVFKVDLLKDAFIRNSDITFTDEASLIEAVGHTVSSIQGDRDNIKITFPEDILIASI
                     LLKKQLSI"
     gene            complement(890..1939)
                     /gene="queA"
                     /locus_tag="PKY09_10805"
     CDS             complement(890..1939)
                     /gene="queA"
                     /locus_tag="PKY09_10805"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNB13386.1"
                     /translation="MKLSQFKFGLNENLLAPHPAANRDESRLMVIDRKTGKIEHKIFK
                     DIINYFDEGDVMVMNNTKVFPARLYGNKEKTGAKIEVFLLRELNPESKLWDVLVDPAR
                     KIRIGNKLYFGEDDMLVAEVIDNTTSRGRTLRFLFDGTSADFREVIEKLGNTPLPKYI
                     KRKAEPEDKERYQTVYAKNEGAVAAPTAGLHFSKQLLKRLEIKGINLAELTLHVGLGT
                     FRPVEVEDLTKHKMDSEWFSITQPCVDIVNKAKAGKHRICAVGTTSMRAMESSVSTLG
                     ELKPFEGWTNKFIFPPYDFSLANCMVTNFHMPESTLLMMVSAFAGYDLTMHAYKLAMK
                     EKYRFYSYGDAMLII"
     gene            2362..2664
                     /locus_tag="PKY09_10810"
     CDS             2362..2664
                     /locus_tag="PKY09_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB13387.1"
                     /translation="MKKTGEIIREKREKKRLLLRHVSAQLDIDTAILSKIERGERKAT
                     REQITKLAEILELDKESLLIQYLSEKILYEIQDEDLGIQALKVAEKTIKYRVTKNK"
     gene            2666..4243
                     /locus_tag="PKY09_10815"
     CDS             2666..4243
                     /locus_tag="PKY09_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNB13388.1"
                     /translation="MAELLSIKEASQWATEYLDKNVTTSNISYLIQYGRIKKIGENGA
                     TQVIKEELIEYYKDQKKSRQEEWKEQLGEDLNWALSFEQYKEAETTKHVHRLHPYKGK
                     FIPQLVEYFLDNHTDKFKKEVYFKPGDIVYDPFSGSGTTMVQSCELGIHAVGNDVSAF
                     NAMIGNCKVTKYNLVDVQTEIDRISKALKSFLADHKTLEFEDRLLKELYVFNNKYFPV
                     PEYKYKVKRKEIDEKTYGEEKEKEFLPTFNKLVKEYKIKLRQDKEDSFLDKWYTQHIR
                     DEIQFVFDEIKKIKNADTKKIVSVILSRTIRSCRATTHADLATLLEPITATYYCGKHG
                     KMCKPLFSILKWWETYSKDTVKRLAQYDKLRKDTFHTCLTGDSRNIDLVGGLYKINPD
                     FAELVEKQKIKGIFSSPPYVGLIDYHEQHAYAYDLFGFERKDELEIGPLFKGQGREAK
                     QSYIQGLSDVLNNAKKYLVYDYDIFLVANDKYNMYPTIAENAGMQIVNQYKRPVLNRT
                     EKDKGAYAEIIFHLKSK"
     gene            4243..5046
                     /locus_tag="PKY09_10820"
     CDS             4243..5046
                     /locus_tag="PKY09_10820"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HNB13389.1"
                     /translation="MSLSIKAKKEINTLIEAVVKKYISKAASKPKANSGNPFVMALLK
                     DFEPLIHRIHGLKTSLGSEMEKIAEIIAIDAWGKANIQRKVKVNVALPKNVFQTIDSI
                     INNLSNAKTLSDYDKEKKMILDACKKPSKTTEQHTYEFDLVVTDPKKKHTYILEMKGP
                     DPNTTEVPGAKKRLLVAIAWAYFKTKSEKIDSHFAIYYNNKHPKVYKNPKVHYYFNPA
                     GGTIVQEDFWNFLGKNTQTFNSLTKLFESYGKTNKKKIWDGFSKLIDIK"
     gene            5046..5828
                     /locus_tag="PKY09_10825"
     CDS             5046..5828
                     /locus_tag="PKY09_10825"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HNB13390.1"
                     /translation="MALTQQQIQQVEAVLRNSLRHKFQNYYPEPAVMPFHTRLLGQDR
                     MALFSFIHSLNTNFGTSIFEPVAKALALSTFASAESQQTAGNQISSEAHRVIQNIMDG
                     LAIATSSPNKIEEINAIREVCQSGEMKTVKPTKVDVKLVGHDGTIYLFDIKTAKPNAG
                     GFKEFKRTLLEWVATTLAANPTANVQTIIAIPYNPYEPQPYNRWTMRGMLDLDNELKV
                     AAEFWDFLGGQGAYTDLLDIFERIGLELRPEIDAYFARYNRQ"
     gene            6169..6897
                     /locus_tag="PKY09_10830"
     CDS             6169..6897
                     /locus_tag="PKY09_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB13391.1"
                     /translation="MVQEINIKVLLVKHNQDLFVPITKCLESESIQYTCVCSATEALN
                     ELKNETYDFILLENQLTEYSALIFLKQLNLQRYQGNVMIVAKNATDEEIVSAISNGSD
                     DVFTTPFNTNLILARLKSLYRLRSGNIPQNYFFGEIEINPTEYKVWVNKNKMTLTKTE
                     FEILKFLIFNPDRIIPHEVLVKQALNLSPSEIGENYEFLYSHLKNLKRKIGQHSAFNY
                     IKTVYGQGYRFCSFIPLAESNAGQ"
     gene            complement(6922..7368)
                     /locus_tag="PKY09_10835"
     CDS             complement(6922..7368)
                     /locus_tag="PKY09_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNB13392.1"
                     /translation="MIELNLPHFDFKIKTEGQSKQIFDQLRNKYVALTAEEWVRQHFI
                     MFLINEKKYPKGLFAIEKEHVFNDLKMRADLIVYKHDLSPWMLCEFKAPEVAITQEVF
                     YQAARYNLTFDVPYLIVSNGLEHFCCKRVEDRFEFQDDLPDFEKVD"
     gene            7441..8211
                     /locus_tag="PKY09_10840"
     CDS             7441..8211
                     /locus_tag="PKY09_10840"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNB13393.1"
                     /translation="MQSKEEIVKNWLPRYTGTAIEDFGKYILLTNFNNYVEIFAEQHQ
                     VEIMGTHRSMPNATANGITIINFGMGSANAATIMDLLTAIDPAACLFLGKCGGLKRKN
                     ELGDLILPIAAIRGEGTSNDYMPAEVPALPAFSLQKAVSTTIRDYDKDYWTGTVYTTN
                     RRVWEHDEEFKQYLRKMRCMAIDMETATIFTTGFVNQIPSGALLLVSDQPMIPDGVKT
                     EKSDLNITRKFVKDHINIGIDSLNQLKNNGLTVKHLRF"
     gene            8218..9282
                     /gene="holA"
                     /locus_tag="PKY09_10845"
     CDS             8218..9282
                     /gene="holA"
                     /locus_tag="PKY09_10845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNB13394.1"
                     /translation="MPERKFEEILKDLKNKVYHPLYFLQGEEPYYIDAISDYVEKHLL
                     NDMEKEFNQTILYGREADAMTIVSTARRFPMMSNYQVVIIKEAQDVKWFGSKEKEEEK
                     TKDKVKEKEDPMMLYLQNPTTTTILLFCYKYKTIDNRTKKGKLIKDKAQFLETKKLYD
                     NQLPAWIKNYLKERKIKADDHAVEMLAEYLGNDLSKINNEVGKMLINLKDKSVITIND
                     IREHIGVSKDYNVFELQNALAERNILKANRIVNYFEANSKNNPIQLIVGSLFAYFNKI
                     LIYHFTTDKSSASLASAMGISPFFIKDYERAARNYPVWKLEQIIGWLREYDLKSKGVG
                     STDQVPTGELLREMVFKIIH"
     gene            9285..10016
                     /locus_tag="PKY09_10850"
     CDS             9285..10016
                     /locus_tag="PKY09_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNB13395.1"
                     /translation="MIRVSVVSYLNSKPFVAGLTQNSIINKIDLSLDIPSTCAEKLIN
                     GKTDIGLIPVAVLPLVKNAQVITPFCIASNGNVNSVLLLSQTPLNEIKQIWLDYQSRT
                     SVMLAQLLAKAFWKIDVSWLPSENGFENKIEGQTAGVVIGDRALALKSNFKYVYDLAT
                     EWKKHTGLPFVFACWVSNKSLPEDFIDAFSDALNQGVALIPELSKQLQPDYPFDVKGY
                     LTENIDFRLDKEKEKALKLFLEQIK"
     gene            complement(10076..10597)
                     /locus_tag="PKY09_10855"
     CDS             complement(10076..10597)
                     /locus_tag="PKY09_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNB13396.1"
                     /translation="MKNSDIIFKDSVKDHYRSLRGFALKLTQNSEDANDLIQETMLKA
                     LNNEDKFEKGTNLSGWLYTIMRNIFINNYRRMVKSQTFLDSTENQFFLNNIKNATRNR
                     GEANLVMKEINNAINDLSDNLKVPFMMSFRGYKYEEIASQLSIPLGTVKIRIHTARQK
                     LKEKLNVYNSLSF"
BASE COUNT         3763 a         1861 c         1879 g         3150 t
ORIGIN      
        1 gtttatcatt taatgctttg aattttatat tgcttcaaca agtgatttac acaactcaat
       61 gttgatagta cttaaacgta aaaaacattg ctatcgtttc tttcaccatc aaatattttt
      121 atttaatgta atgatttgcg aaataaaatt acacaaattt ttatgaattc ttacaagaga
      181 aaataagtgc ccacttcaga tggataattg cttcttgagc agtatggatg caataagaat
      241 atcttccgga aaagtgattt taatattatc tctgtcgccc tgaatggaag atacagtatg
      301 tccaacagcc tcaattaatg aagcttcatc tgtaaaagta atgtcgctat ttctgataaa
      361 agcatcttta agcaaatcaa ctttaaagac ctgcggtgtt tgtacagcga aaatttcagc
      421 cctattgaca gccttattct ccgctgaatt aattcgcctt aacgactctc tgacagctac
      481 aacgggtatg gcattacccc tgtgagcagc atgctcaaag agtttttctg ccagttccgg
      541 ttttaaaatt ggccttgctg catcatgcac agcaactaag gcttcattat cgtcaatggt
      601 ttgtaagcca ttaaatacag aacggtttct ggttaaacct cccgaagaaa tcttatgcgg
      661 tactttaaaa tgatattctt gaatcaatga ttgccaatgg ctatgttcaa tatcaggcaa
      721 aaccactgta acatctgcac ctacacgatg caaacattca atagaataca tgagtacggg
      781 tcgtccttca agcaacaaaa actgcttagg aacattggct tgcattcggg ttccactgcc
      841 tccggcaact attattgcaa atcgcttttg gctcataata aataaactat cagataatca
      901 gcatggcatc gccatagcta tagaagcgat atttttcttt catcgctaat ttataagcat
      961 gcatggtaag atcataacct gcaaacgcag agaccatcat caacaaagtt gactcaggca
     1021 tgtgaaaatt agttaccata caattggcaa gactaaaatc ataaggcgga aaaataaatt
     1081 tattagtcca gccttcaaat ggtttaagtt ctcctaatgt agaaacggat gactccatag
     1141 cgcgcattga cgtagtgcct actgcgcaaa tacgatgttt accggccttt gctttattaa
     1201 ctatgtcaac acatggctgc gtaatactaa accactctga atccatctta tgcttggtaa
     1261 ggtcttcaac ctcaacaggc ctgaatgtcc ctaatccaac atgcagtgtc aactcagcaa
     1321 gattgattcc tttaatttca aggcgcttta atagttgttt gctgaagtgt agtcctgcag
     1381 taggcgctgc aacagcacct tcgtttttgg cataaacagt ctgatagcgc tctttatctt
     1441 ctggctcggc cttacgtttg atatatttcg gaagtggcgt attacctaac ttctcaataa
     1501 cctctctgaa gtcggcactt gtgccatcaa acaagaaacg caaagtacgg ccacgtgaag
     1561 ttgtattatc aattacctcg gcaacaagca tatcgtcctc accgaagtac aatttatttc
     1621 caatacgtat tttacgtgca gggtccacaa gcacatccca gagtttactt tcaggattta
     1681 gctcacgcaa taaaaagact tcaattttag cacctgtttt ttctttattg ccataaaggc
     1741 gtgcaggaaa aaccttggta ttattcatca ccatcacatc gccctcgtca aagtaattaa
     1801 tgatgtcttt aaagatttta tgctcaatct taccggtttt acggtcaatt accattaacc
     1861 tactttcgtc acggttggca gcaggatgtg gagcaagaag gttttcgttt aggccgaatt
     1921 taaattgtga taatttcata tctgtaatct tacaggatta ttttttgagg gcgcaaatat
     1981 ataccaagca gaagccgatg gcgaaaaaaa gttataagtt attgtatatc aaagtaaaat
     2041 attttaaaag cacaactttt tggtgaatta aatagtgaaa tgctgcttat gttcatcaga
     2101 aaaaattgta gcatagaaca agacaaccaa atgacttcaa taaactactt aaataaggtt
     2161 gacatgtcct gaaagctttc gggagcacat ttgcaaaaac ccaagcttgc taaagaactt
     2221 gcactccacc actccaaaac cacccaccac ccgacttctt gacaagtttg cagactgttg
     2281 ataaaatctt taaagtttat tgcgaccaaa tcaaaaagtc tttttacatt tgacaattat
     2341 tgtcaagtta atattttcaa attgaaaaag accggagaaa taatcagaga gaaaagagaa
     2401 aaaaaaaggt tgctattgcg acacgtttct gctcaactcg acatagacac agctatactc
     2461 agtaaaattg aacgtggaga gagaaaggcc acaagagagc aaattactaa gttggctgag
     2521 atacttgaac tcgacaaaga atccttgctt atccaatatt tgagtgagaa aattctttat
     2581 gaaattcaag acgaagactt gggaattcaa gcacttaaag ttgctgaaaa aacaattaaa
     2641 tacagagtaa ctaaaaacaa ataaaatggc agaattacta tcaataaaag aagcaagcca
     2701 gtgggcgaca gagtatttgg acaaaaatgt tactacttct aacatttctt atctaatcca
     2761 atacggaagg ataaaaaaaa ttggtgaaaa tggtgcgact caagtaatca aagaagaatt
     2821 aattgaatat tacaaagacc aaaaaaaatc aagacaagaa gaatggaagg aacagcttgg
     2881 tgaagatttg aattgggctt tatcatttga acaatacaag gaagcagaaa cgacaaagca
     2941 tgttcacaga cttcatccat acaagggaaa atttattcct cagttagttg aatatttcct
     3001 tgacaaccac acagacaaat tcaagaagga agtttatttt aagccgggtg acattgttta
     3061 cgacccattt tcaggaagtg gaactacaat ggttcaatct tgtgagttag gaatacatgc
     3121 ggttggcaat gatgtttctg catttaatgc aatgataggt aattgcaagg ttacaaaata
     3181 taaccttgtt gatgttcaaa ctgaaataga cagaatttct aaagcattaa aatcattttt
     3241 agcagaccac aaaacgcttg aatttgaaga cagactttta aaagagttgt atgttttcaa
     3301 caacaagtat tttccagttc cagaatacaa atacaaagta aaacgaaaag aaattgacga
     3361 aaaaacttat ggcgaagaga aggaaaaaga attcttgcca acattcaata aacttgtaaa
     3421 ggagtataaa ataaaacttc gccaggacaa agaagattcg ttccttgata aatggtatac
     3481 tcaacacatc cgtgatgaaa ttcaatttgt gtttgatgaa atcaaaaaga taaaaaatgc
     3541 cgataccaaa aaaattgtta gtgtaattct aagtcgcacc attagaagtt gcagagcaac
     3601 tacacatgct gatttagcta cattgcttga gccaatcaca gcaacatatt actgcggtaa
     3661 acatggtaaa atgtgtaagc cattgttttc aattcttaaa tggtgggaaa catattctaa
     3721 ggacacggta aaaagattag ctcagtatga caagctaaga aaagacacat ttcatacttg
     3781 tttaacaggt gacagcagaa acattgacct tgtaggagga ctctataaaa taaatcccga
     3841 ttttgcagaa cttgtagaaa agcaaaaaat caaaggaata ttttcatcac caccttatgt
     3901 tggtttaata gactaccatg agcaacatgc ctacgcttat gacttgtttg gctttgaaag
     3961 aaaagacgaa ttggaaatcg gaccactttt taaaggacaa ggcagagaag caaaacaaag
     4021 ctatattcaa ggtctaagtg atgttttgaa taacgcaaaa aaatatctgg tctatgatta
     4081 tgatattttc cttgtagcaa acgacaaata caatatgtat ccgaccattg cagaaaatgc
     4141 cggaatgcaa atagttaatc aatataaacg accagtatta aaccgaactg aaaaagataa
     4201 aggtgcttat gctgagataa tttttcatct aaaaagtaaa taatgagttt atccatcaag
     4261 gcaaaaaagg aaataaacac cttaatcgaa gctgtagtaa aaaaatacat cagtaaggct
     4321 gcaagtaaac caaaagctaa ttcaggcaac ccgtttgtaa tggctttact taaagacttt
     4381 gagcctctta ttcacagaat acacgggctt aaaacttctt tgggaagtga aatggaaaaa
     4441 atcgcagaga taattgcaat agatgcttgg ggtaaagcaa acattcaaag aaaagtaaaa
     4501 gtgaatgttg ctctacctaa aaatgttttt caaacgattg actcaattat caataatttg
     4561 agcaatgcca agactcttag tgattatgat aaagagaaaa aaatgatact tgatgcttgt
     4621 aaaaaaccat ctaaaacaac tgaacaacat acttatgaat ttgaccttgt tgtaacagac
     4681 ccaaagaaaa agcacactta catccttgaa atgaaaggtc ctgacccaaa tacaactgaa
     4741 gttccaggtg ctaaaaagag attacttgtt gcaattgcct gggcttactt caaaacaaag
     4801 tcagaaaaaa ttgattccca ttttgcaatc tattataaca acaagcatcc aaaagtttac
     4861 aaaaacccta aagtgcatta ttactttaat ccggcaggcg ggactattgt tcaagaagat
     4921 ttttggaatt ttcttggaaa aaatactcaa actttcaact ctttaaccaa actatttgaa
     4981 tcatacggta aaactaacaa aaagaaaatt tgggatggct tttcgaaact aattgatata
     5041 aaataatggc attaacacaa caacaaatac agcaagttga agcagttcta cgaaacagtt
     5101 taagacataa attccaaaat tattatcctg aacctgcagt gatgcctttt catacaaggc
     5161 ttcttggtca ggacagaatg gctttgtttt catttatcca ttcattaaac acaaatttcg
     5221 ggacaagcat ttttgaacct gttgctaaag cgttggcatt atcaactttt gcaagtgccg
     5281 aatcacagca aacggccggt aatcaaattt cttcagaagc tcatagagta attcagaata
     5341 taatggacgg tcttgcaatt gcaacttctt caccaaataa gattgaagaa ataaacgcaa
     5401 tcagagaagt atgtcaatcg ggtgaaatga aaacagtaaa gccaaccaaa gtggacgtta
     5461 aacttgttgg gcatgacgga acaatttatt tattcgacat caaaacagcg aaaccaaatg
     5521 caggcggatt taaagaattt aaaagaacac ttttagaatg ggtagcaaca acattagcag
     5581 ctaatccgac agcaaatgtt cagactatta ttgcaattcc ttacaatcct tacgaaccgc
     5641 aaccatacaa ccgttggacg atgcgtggaa tgctcgactt agacaacgaa ttaaaagttg
     5701 cagcagaatt ttgggacttt cttggcggac aaggtgcata tactgacctg cttgacatat
     5761 ttgaacgaat cggtttagaa ctaagaccag aaatagatgc atactttgcg agatataaca
     5821 gacaataacc aacgtatttg ttgacactga cttgtcttga aacaggttgt tttcatttct
     5881 ttaaaaatca aactggctca cagaaacttt agtgtaatag tgctgtttat ggcctgattt
     5941 ttgttgtagt tttcgtgaag ttactaataa attttaccac ccaccttttt ctaaaagtcg
     6001 taacattttt tttaaatgtt aataagttat ttggtgaacg tccatttttt atgggtgaat
     6061 acttaatatt gctgcataag tgagtaaaat ctcatgcatg cttacctatc ttaaatgtag
     6121 ttggcagttt aaactaatta ggatcaacaa caaacataac aaccatatat ggtacaggaa
     6181 atcaacatta aagttttatt ggtaaaacac aatcaggact tatttgtgcc aattaccaaa
     6241 tgccttgaaa gcgaaagtat tcaatatact tgcgtctgct cggcaactga agcacttaat
     6301 gagctaaaaa acgaaactta tgattttatt ctgctggaaa atcaacttac agagtacagc
     6361 gcgctaatct ttttgaaaca acttaatctg caacgctatc agggcaatgt gatgattgta
     6421 gcaaaaaatg caactgacga agaaattgtt agtgccatca gcaatgggtc tgatgatgtt
     6481 tttacaacac catttaatac caacctgata cttgcccgtt tgaaaagcct ttatcgttta
     6541 agatcaggca atatccctca aaactatttt tttggtgaga tagaaattaa cccgacagaa
     6601 tataaagtgt gggtcaacaa aaataaaatg accttgacaa aaacagaatt tgaaattctt
     6661 aaatttttaa tttttaatcc ggacagaata attccacatg aggtgttagt caaacaggca
     6721 ttgaacttat ccccttctga gattggagaa aattatgagt ttctatacag tcatctgaaa
     6781 aatctgaaaa gaaaaatagg acaacattcg gcttttaatt atataaaaac tgtttatggt
     6841 caaggatatc gtttttgttc ctttattcct ttagcagaaa gtaatgccgg gcaataaagt
     6901 attaatcatc aagcaacttt attaatctac tttctcaaaa tcaggaaggt cgtcctgaaa
     6961 ttcaaaacgg tcttctaccc ttttgcagca aaaatgctct aacccattac ttacaataag
     7021 ataaggcaca tcaaaagtca gattgtagcg tgctgcctga taaaatactt cttgtgtaat
     7081 agcaacttca ggggctttaa actcgcaaag catccatggt gataaatcat gcttataaac
     7141 aatcaggtcg gcacgcattt tcaagtcatt aaaaacatgc tctttttcaa tggcaaacaa
     7201 tcctttcgga tatttttttt cgttaatcaa gaacatgata aaatgctgac gcacccattc
     7261 ttccgctgtt aaggctacat acttatttct aagctggtca aaaatttgtt tactttgtcc
     7321 ctctgtttta attttaaaat caaaatgagg taaattcaat tcaatcattg aacgaaatta
     7381 aataaactca ttgaaattat tgtttaaaca gatgattcag ctatcaagaa ataaaatttt
     7441 atgcaatcaa aagaagaaat tgtaaaaaac tggctcccgc gttacacagg tacagctatt
     7501 gaagattttg gcaaatacat tttgctcacc aactttaata attatgttga aatttttgcc
     7561 gaacaacatc aggtagaaat tatgggcaca catcgttcca tgcctaatgc tactgcaaat
     7621 ggtattacaa taattaattt cggaatgggc agtgccaatg ctgcaacaat aatggatttg
     7681 cttacagcca tagaccctgc tgcttgttta tttcttggaa aatgtggcgg attaaaacgt
     7741 aaaaatgaat tgggcgattt aatacttcct attgctgcta ttcgtggcga aggaacatca
     7801 aatgactata tgcctgctga agttcctgcc ttgcctgcat ttagtctgca aaaagctgtg
     7861 tcaacaacga taagagatta tgataaggat tactggacag gaaccgttta caccaccaac
     7921 cgaagagttt gggagcatga tgaggagttt aaacaatacc tgcgcaaaat gcgttgtatg
     7981 gctatagata tggaaacagc aacaattttt acaactggat ttgtaaatca gattccatcg
     8041 ggagcgctat tgctggtaag cgatcagcca atgatacccg atggtgtgaa aacagaaaaa
     8101 agtgacctta atattacaag aaaatttgtg aaagatcata tcaatattgg aatagattca
     8161 ttgaatcagt taaagaataa cggtctaacg gtaaagcatc tgcgttttta attaactatg
     8221 cctgaacgca aatttgaaga gatattaaaa gatcttaaaa acaaggttta tcatcctctg
     8281 tattttctgc agggtgaaga gccttattac attgatgcca tcagtgatta tgttgaaaaa
     8341 catctgctca atgacatgga gaaagaattt aatcaaacta ttctttacgg tcgcgaagct
     8401 gatgccatga caatagtgag tacagcacgc agatttccaa tgatgtccaa ctatcaggtt
     8461 gtaattataa aagaagcaca agatgtaaaa tggtttggca gcaaggagaa agaagaagaa
     8521 aaaacaaaag ataaagtcaa ggaaaaagaa gacccgatga tgctttatct tcagaatccg
     8581 acaactacaa ccattctgtt gttttgctat aaatacaaga ccattgacaa caggaccaaa
     8641 aaaggaaaac tgataaagga taaagcacag tttcttgaaa caaaaaaatt atatgacaat
     8701 cagttgcctg cctggataaa aaattatctg aaggaaagga aaattaaagc tgatgaccat
     8761 gctgtagaaa tgcttgccga atatttaggg aatgatttat ctaaaatcaa caatgaagtg
     8821 gggaaaatgc tgattaatct taaagacaaa tcggtaatta caataaacga tattcgtgag
     8881 catattggag tcagtaaaga ctataatgtt tttgaattac agaatgcatt agcagaacga
     8941 aacattttaa aagcaaacag aattgtaaat tattttgaag cgaattcaaa aaacaatcct
     9001 attcaactga ttgttggcag tttgtttgct tacttcaata aaattctgat ttatcatttc
     9061 actaccgaca aatcttctgc atcacttgct tctgcaatgg gcataagtcc attttttatt
     9121 aaagattatg aacgtgctgc cagaaactat cctgtctgga aattagagca gattatcggt
     9181 tggctacgcg agtatgattt aaaatcgaaa ggtgttggca gtacagatca ggtgccaaca
     9241 ggtgaattgc tcagagaaat ggtctttaaa attattcatt aaaagtgatt cgggtatctg
     9301 tagtttctta tctcaactcc aaaccatttg ttgccgggtt gacacaaaat agcatcataa
     9361 acaaaattga tttgtcatta gacattccat ctacttgtgc agaaaaacta atcaatggaa
     9421 aaacagatat cggattgata cctgtagccg ttctgccttt ggtaaaaaat gcacaggtaa
     9481 taactccgtt ttgtattgct tcaaatggta atgttaactc agtgttgttg ctgagtcaga
     9541 ctccgttaaa tgaaataaaa caaatctggc ttgattacca aagccggact tcggtaatgc
     9601 tggcacagtt gcttgcaaaa gcgttctgga aaattgatgt tagctggctt ccttctgaaa
     9661 atggatttga aaataaaata gaaggtcaaa ctgcaggagt tgttataggc gacagagcat
     9721 tggcgttgaa gtcgaacttc aaatatgtgt atgaccttgc cacagaatgg aaaaaacata
     9781 caggcctacc ctttgtattt gcttgttggg tgagcaacaa gtcattaccg gaagatttta
     9841 tagatgcatt ttctgatgcg ctcaatcagg gagtagcatt aattcctgag ttaagcaaac
     9901 aattacagcc ggattatcct tttgatgtaa aaggctattt aacagaaaac attgacttca
     9961 gattagataa agaaaaagaa aaagcattga aattgttttt ggaacaaatt aagtgataga
    10021 aaatcaattc gcctttatca aatattcttt tgaacgtaat ccgaaaacta tcttcttaaa
    10081 acgacaatga attataaacg ttaagttttt ctttcagttt ttgtcttgca gtatgtattc
    10141 tgatttttac ggttcctaat ggaatagaca attgcgaagc aatctcttcg tatttgtatc
    10201 cccggaaact catcataaaa ggcactttaa gattatcgga caagtcgttg attgcattgt
    10261 tgatttcctt cattaccaag ttagcctctc ccctgtttct ggtagcattt ttaatgttat
    10321 tcaggaagaa ttgattctct gtactatcta aaaatgtctg actttttacc attctgcgat
    10381 aattgttaat gaaaatattt cgcattatgg tgtaaagcca tccgctaaga ttggttcctt
    10441 tttcaaattt atcttcgttg tttaatgcct ttaacattgt ttcctgaata aggtcgttag
    10501 catcttcaga attctgagtg agttttaatg caaaacctct taaagaacga tagtggtctt
    10561 ttactgagtc tttaaatatg atatctgagt ttttcatttg tttaaccttt ttaaattaaa
    10621 gctcaacaaa cttacgatgc aaacatgaat tat
//
LOCUS       DAOQNP010000090         9750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2538_length_9750_cov_9.030222, whole genome shotgun sequence.
ACCESSION   DAOQNP010000090 DAOQNP010000000
VERSION     DAOQNP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2538_length_9750_cov_9.030222"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3221)
                     /locus_tag="PKY09_10860"
     CDS             complement(<1..3221)
                     /locus_tag="PKY09_10860"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNB13397.1"
                     /translation="MWTTSAQAQTTITTTFANNNGSSVVVFSVQNNNPYAIIMTDIGS
                     HAGVTSTYTCYLYAKAATYNVAPGAAGAITAANGWSVVASNSSLSLPANTSGTGSTAS
                     PFITGMSYTIPAMTQVRLALQLATGAGLPPFTTTAGSLRYSTVSGTCSFTGAGVDLNS
                     CASYGYGGTLSSPTNTPRGLVGYISFIPAVPCTGTPTPGNTTANVNPVCPSSSFTLGL
                     QNPSNGSGVTYQWQSSPDNITYTNISGATSASYALTQSVDTWYQCIVTCTNSGQTTTS
                     NPLQVTTNPFYNCYCTSTATSTADEDILNVTFGSLNNTSTCSTTGTGPGSVLNMYSNY
                     RSGVGAPAAPSVMQGDVVPLSLGLGTCGGSYPNTAKVFIDWNQNGLFTDAGEEVYVST
                     SGSGARTVTGSVTVPLSAVPGNTAMRVVFVETSTPVPCGTYTWGETEDYLINVVATSA
                     CSGAPVAGTASGPASICPSIAFTVSTSGATVGTGISGQWYSSTDGGSTWSPISGATST
                     SYNVAAGISVPTMYQYVITCSNSGLSATSNNISIAVNPVNACYCTPTSTSATYGITNL
                     TTTGGIANINNTSPGGQGYVDNTAQAVSQLQGSSVNFSYTVVPTEGVGIWIDWNQNGS
                     FLDAGEQVYNAGSYVSSGSGTISVLLSQPAGNYRMRIVGNWLSTSPTPCGDLGSAGYG
                     EAEDYTFTVIPLSNCSGTPAPGNTLSNPATACIGQNINLSLQNSVVGAGITFDWQSSP
                     DGVTWTSTGGTLPSYTTTQSAATWYQCIVTCTNSGQSATSTPVQVTMSPFYNCYCTTG
                     LGGSCAINSLSSVAIATTTLNNVTGGCTGTYNSFPASGSTTASLLPGVPYTMTAGCIS
                     GSNTQVAIWIDYNQNGIFETTEYTLINGNIPSGGTGSGTFTIPVTALSGNTGMRVRSD
                     WQGTTAWTSADACTNRTWGETEDYTITVLVPNSCSGAPASSATTSSASGVCPGGSFTL
                     GTNPVYTDAGLTFDWQSSPDGITYTSTGVTTQSYTVTSYAATTWYQCVVTCTNGGLST
                     TSTPIQVLLNPFYNCYCTTSLHSSSTPCINEIDLNTLSNNTAAA"
     gene            complement(3760..4974)
                     /locus_tag="PKY09_10865"
     CDS             complement(3760..4974)
                     /locus_tag="PKY09_10865"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNB13398.1"
                     /translation="MLTVDDFNFNGKRALVRVDFNVPLDKSTFSVTDNTRIKAAVPTI
                     KKILADGGSVVLMSHLGRPLKKLKDDGTVDTKKFTLKHTLSEVSKLLGVEVKFASDCV
                     GAETSVLASKLQPGEVLLLENLRFHKGEEKGEEDFARQLASLGNIYVNDAFGTAHRAH
                     ASTAIIAQFFPGAKMFGYVMANEIKNAERIMQHAEKPFTAIMGGAKVSDKILIIEQLM
                     LRCNHILIGGGMAYTFFKAMGGNIGSSLCETDKLDLAKQLLERAKQSGVQFHLPFDSV
                     LGDKFDNDAQTSISDNMSIPDGWMGLDIGPKAVTAFSEIISKSKTILWNGPMGVFEME
                     KFAAGTKGIADAIVSATSKGAFSLIGGGDSAAAINKFGLADKVSYVSTGGGALLEYFE
                     GRELPGIKMIMS"
     gene            5171..6307
                     /locus_tag="PKY09_10870"
     CDS             5171..6307
                     /locus_tag="PKY09_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13399.1"
                     /translation="MQFKDVVGQKDTKQLLLKMAGENRIPHAMLFMGNAGSGTLPLAI
                     AFAQLINCENATTDDSCGVCHSCRMYAKLSHPDLHFALPVNAIDDIKKPKTDDFIEQW
                     RAAYIKNPNLSLQDWYDTIGIEKKQGIIATEESAAIVHKLYLKSFEGKHKVLIMWHAD
                     RMNSATSNKLLKIIEEPPGNTVFILITERYDGILPTILSRTQLVKIPKIDNDSLIKYA
                     CEKYGLDINKARNMVHLSGNDITELMALMNQENLSGQMEHSFIEWMRMCYKFGTNYKV
                     LYSWIEDISKLSRDAQKNFLSFCLGAARECLITNMAGINHSRYTDATFNGFERFSKMI
                     NADNVEPFAKTLEEAIYHIERNANPKILFLDLSFRMHRILHMKF"
     gene            6315..7502
                     /gene="ricT"
                     /locus_tag="PKY09_10875"
     CDS             6315..7502
                     /gene="ricT"
                     /locus_tag="PKY09_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNB13400.1"
                     /translation="MTTTTINTNTKSTGCTSCGTGSCNKLNVYDWLSDMEFPPGHIPF
                     NVVEVQFKGTRKEYFRNTDGHILKAGDIVAVEGNPGHDIGTVTMTGELVKLQLKKRGF
                     ESGAPEIKQLYRPAKQGDIEKWEQVKQLEHQTMHRARKIALQLGLKMKLSDVEYQGDG
                     KKATFFYTAEERVDFRELIKKLHEEFKVRIDMRQIGLRQEAGRLGGIGSCGRELCCST
                     WMTSFKTVGTSAARYQNLSINPVKLAGQCGKLKCCLNYELDSYMDAIKDIPEQGTAIF
                     TEAGKALHRKTDIFKRIMWYELIPNLKDGEYYQPSADKWVMLPVDRVKEIIALNKANQ
                     KPADLKSGLPVEDEAEEPDYADMVGQDSLTRMDKKKKKKKKKKKPSQPNQQTNNGQQK
                     KPS"
     gene            7477..7959
                     /locus_tag="PKY09_10880"
     CDS             7477..7959
                     /locus_tag="PKY09_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNB13401.1"
                     /translation="MANRKSLLRYLIAGVVIVVFASCNQQYHFEENKSIKGSNWDFSE
                     PISFDVNIDDTLTAYNMYINVRNQGNYRFSNLFLFINTHLPQGQHLRDTVEITLATPD
                     GAWLGKGIGDLYSNRYLFRKNFRFPQKGDYHVELIQAMRVNPLPGILDAGIRIEKTKQ
                     "
     gene            complement(8050..8892)
                     /locus_tag="PKY09_10885"
     CDS             complement(8050..8892)
                     /locus_tag="PKY09_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13402.1"
                     /translation="MRKRYIYLSTVIAGLLLTGSCSNKSGEESKEQTAETKDTMPVSE
                     VATFQFTYTIANLPPPMQVLDEFSKSKLEVNTALLNSADNADKYMTSLKQAMNYGIYG
                     VDLGYLVVNNRTLDAIKYYNTAKKLASQLGMEETFNQFVNRFETNSNNRDSLTRVIDD
                     AYAATDAYLRSNKRLETASQVLAGSWLECQYLTVKSLLTASRNADNEILYKRVWEQRL
                     YLDNITKLLSEFADNDEVKKIKSDYEALLAIYKEPSDEKQIDKAFLTRLASGLEKARQ
                     NIVE"
     gene            complement(9425..>9750)
                     /locus_tag="PKY09_10890"
     CDS             complement(9425..>9750)
                     /locus_tag="PKY09_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13403.1"
                     /translation="KMFKSPKQIFTRIHTHPLTKKFSMVYDLYTYYSKYEHFGIMTHF
                     MQRQNINQDLDRIIWSISYIIRGIGATFAYLSYPLDKLQGEKEALGKLQIEFDKLGAK
                     EEQPA"
BASE COUNT         3019 a         1906 c         1861 g         2964 t
ORIGIN      
        1 gcagcagctg tattgttact taaggtattt aaatcaatct cattaataca aggtgtacta
       61 gaagaatgta aacttgttgt acagtagcaa ttataaaatg ggtttaacaa tacttgtatt
      121 ggtgttgatg tagttgacaa accaccgttt gtacaagtta ccacacattg ataccatgtg
      181 gtagcagcat aacttgttac tgtataggat tgtgtagtta ctccagtact ggtataggta
      241 ataccatcag gagagctctg ccagtcgaat gttaaaccag catctgtata tacagggttg
      301 gtgcctaatg taaagcttcc tccgggacat acaccagaag ctgatgaggt tgtagctgat
      361 gaagcaggtg cacctgagca ggagtttgga accaatacag taatggtata atcttctgtt
      421 tcaccccatg tacggttggt acaagcatcc gcagaagtcc atgctgttgt tccctgccag
      481 tcacttctta cacgcattcc ggtattacca cttaaggctg ttacaggtat tgtaaatgtt
      541 ccactgcctg tgccaccact tggtatattt ccattaatca aggtatattc tgttgtttca
      601 aatattccgt tttgattata gtcaatccaa atggctacct gcgtgttgct tccggaaata
      661 catcctgctg tcatggtata tggtacaccc ggtaataaac ttgccgttgt gcttcctgat
      721 gcagggaatg aattatatgt tcctgtacat ccacctgtaa cattgtttaa ggtagtagtt
      781 gcaatggcaa cactactcaa tgagttgatt gcacaactgc caccaagtcc tgttgtacaa
      841 taacaattgt agaatggact catagtaacc tgaacaggtg ttgaggtagc actttgtcca
      901 ctgttggtac aagttacaat gcactgatac catgtagcag cactttgtgt agttgtataa
      961 gaaggtaatg tacctccggt acttgtccaa gttacaccat ccggtgatga ctgccaatcg
     1021 aatgtaatgc ctgcgcctac cacactgttt tgtaatgata aattaatatt ttgtccgata
     1081 caagccgtag ctggattgga caaggtattg ccgggtgcag gtgtgcctga gcagttgcta
     1141 agaggaataa ccgtaaacgt ataatcttct gcttcaccat aacctgcaga acctaagtca
     1201 ccacatggtg taggagaagt tgagagccag ttacccacga tacgcattct gtaattgccc
     1261 gcaggttggc ttaatagcac agagatagtt cctgaaccac tacttacata gcttccagca
     1321 ttatatactt gctcacctgc atctaagaag cttccatttt gattccagtc tatccagatt
     1381 cctacacctt ctgtaggtac tactgtatag ctaaagttca cactactacc ttgaagttgc
     1441 gacaccgctt gagctgtatt atctacatat ccctgaccac cagggctcgt attattgata
     1501 ttagctatac cacctgtagt agttaaatta gtaataccat aagtagcgct ggtagaagta
     1561 ggtgtacagt agcacgcatt aacaggattt acagctatgc ttatattgtt actggtagca
     1621 ctcaagccac tattactaca agtaatcaca tactgataca tagtaggcac cgaaatgccg
     1681 gctgctacat tatagcttgt gctggtagca ccgctgatag gcgaccaagt gcttcctcca
     1741 tcagtagagg aataccactg accactgatg cctgtaccta cagtagcacc cgaagtgctt
     1801 accgtaaagg caatactcgg acatatactt gcagggccag atgccgtgcc ggcaaccgga
     1861 gcaccgctac aagctgaagt ggctactaca ttgattaaat aatcttcggt ttcgccccat
     1921 gtataagtac cacaaggaac aggtgtactg gtttcaacaa aaaccacacg catagcggta
     1981 ttgccgggta cggcactcaa aggtacagta acagagccgg taacggtgcg tgcacccgat
     2041 ccagaagtag atacataaac ctcttcaccg gcatcggtaa acaaaccgtt ctgattccag
     2101 tctataaata ctttagcggt atttgggtag ctgcctccgc aagtacccaa acctaaagaa
     2161 agcggtacta catcgccttg cattacgcta ggtgctgcag gtgcgcctac accactccta
     2221 tagttgctgt acatattgag cacagagcca ggacccgtac cagtagtaga gcaggtagaa
     2281 gtattgttca acgaaccgaa agtaacgttt aatatatctt catctgctgt actcgtagca
     2341 gtggaagtac aatagcaatt gtaaaacgga ttggttgtta cctgtaaagg gttagatgta
     2401 gtagtctgtc cactgttggt acaggtaact atacattgat accaggtgtc cactgattga
     2461 gttaaggcat atgatgcgct tgtagcacca ctaatatttg tataagtaat attatcagga
     2521 gatgattgcc actgataggt tactccactt ccatttgatg gattttgtaa ccctaatgtg
     2581 aaagaagaac ttggacatac aggattaaca tttgccgttg tatttccagg tgttggtgtg
     2641 ccagtacatg gtactgctgg aataaatgaa atatatccta cgaggcctcg tggtgtatta
     2701 gttggtgaag ataatgttcc accataaccg taacttgcac aagaattcaa atcaactcct
     2761 gctccggtaa atgaacaagt accactcact gttgagtatc taagacttcc tgcagtagtt
     2821 gtaaatggag gtagccctgc tcctgttgct aattgtaaag caagtcgcac ttgtgtcatt
     2881 gccggaatcg tataactcat acctgttata aaaggcgaag ccgtactacc ggttccagaa
     2941 gtatttgcag gaagagataa actgctgttc gaagctacta cactccaacc atttgcagca
     3001 gtgattgctc cggctgcacc tggagctaca ttataggtgg ccgccttggc atacaaatag
     3061 caagtgtaag ttgatgttac tcccgcatga ctaccgatat cagtcattat gatagcataa
     3121 ggattgttgt tctgcacact aaataccaca actgatgaac cgttgttgtt agcaaacgta
     3181 gtagtaatag ttgtttgcgc ctgtgcgctc gttgtccaca ttcccaccag gaacatgaaa
     3241 caaatcatcc atctccaaca agagaaagaa tgtactctgt ttttgtaatt tgtttttaac
     3301 atgatatttg attttggtta aactaattga tttttattct ctacattaaa tactaaaacc
     3361 gtgtgcagat aagcgataaa aagttgcaaa aaatagctgt aaataagcat ttatttctgt
     3421 ccggttttgg taattcggtt tcaatattat tcatgaaatt tttattttcc aagaaatttt
     3481 taactttctt ttaggaaatt tgatgaaata gatatatgtc taaatattat tgtcgccata
     3541 cacctacaaa acggtttatt agttccttaa aaatagaaaa ggttgctatt taaacaacct
     3601 taaacattac ctacagaaga ttaaaataaa ccggctattt tatttaaaaa tatcaattgt
     3661 gttttatcaa agttaattcc atctgccaat tattacggct attgaaattt attaaatcag
     3721 tttatgataa atagctgaat aataaccttt gttacagact taagacataa tcatttttat
     3781 tcccggcaat tctctacctt caaaatattc caataaagca ccaccacctg ttgaaacata
     3841 acttacttta tctgccaaac caaatttatt aatagcagcc gcagaatcgc caccaccaat
     3901 taatgaaaaa gcaccttttg atgttgcact aacaattgca tctgcaattc ctttagtccc
     3961 tgcagcaaat ttttccattt caaatacgcc cattggtcca ttccataaaa tggttttgct
     4021 tttactgatg atttctgaaa acgctgttac cgcctttggt ccaatgtcta aacccatcca
     4081 accatcagga atactcatat tatcactgat tgatgtttgt gcatcattgt cgaacttgtc
     4141 accaagaaca gagtcaaatg gtaaatgaaa ctgaacccct gattgttttg cccgttccaa
     4201 aagttgtttg gctaaatcta atttatctgt ttcgcaaagt gatgatccaa tatttccacc
     4261 cattgcttta aagaaagtat aagccatacc accaccaata agaatatgat tgcagcgtaa
     4321 catgagttgt tcgatgatga gaattttatc agaaactttt gccccgccca taatagctgt
     4381 aaatggtttt tctgcatgtt gcataatgcg ttctgcattt ttaatttcat ttgccataac
     4441 ataaccaaac atttttgctc ccggaaaaaa ctgtgctatg attgctgtac tggcatgggc
     4501 acgatgtgcc gttccaaatg catcgttaac ataaatatta cccaaagagg ctaattgtct
     4561 ggcaaaatct tcttcgccct tttcttcgcc tttatgaaac ctaaggttct ctaacaatag
     4621 tacttcaccg ggttgaagct tagaagcaag tacagatgtt tctgcaccaa cacagtcgga
     4681 agcaaacttt acttcgacac ccaataactt cgacacttca cttaacgtat gttttaaagt
     4741 aaattttttt gtgtcaacag taccatcgtc tttcagcttt tttaatggcc ttcctaaatg
     4801 actcatcagc actacgctgc caccatctgc caatatcttt tttatagtag gaactgctgc
     4861 tttaattcga gtattatcag tcactgaaaa ggttgattta tctaaaggaa cattaaaatc
     4921 aacacgcact aaggctcgtt ttccgttaaa attaaaatca tctactgtta gcataattgt
     4981 tatgagtaat tttcactgca aagatagtta ctgtcaacta attctatgac ttgatacaat
     5041 atcaaaactt gttgttttgc tgttattaaa aaatcgtatt tgactaaatt gtaaattaca
     5101 atagattaac tgtttaatta tttctgaaaa catcataggt taacgtctaa tgttatctta
     5161 cttttgcagt atgcagttta aagatgtagt aggacaaaaa gacaccaagc agttgctatt
     5221 gaaaatggct ggtgaaaacc ggattccaca tgccatgctt tttatgggaa atgccggaag
     5281 tggtacactg cctttagcca ttgcttttgc tcagttaatc aactgcgaaa atgccacaac
     5341 agacgatagc tgcggtgtgt gtcacagttg ccgcatgtat gcaaagcttt cacatcccga
     5401 tttgcatttt gcactacctg tgaacgctat tgatgatatt aaaaaaccta aaacagatga
     5461 ttttattgaa cagtggcgtg cagcatacat aaaaaaccct aacctttcac tacaagactg
     5521 gtacgacaca ataggtattg aaaaaaaaca aggaataatt gcaacagaag agagtgcagc
     5581 aattgtacat aaactctact taaaaagttt tgaaggcaaa cacaaagtgc ttatcatgtg
     5641 gcatgcagac agaatgaata gtgcaacaag caacaaactt cttaaaataa ttgaagaacc
     5701 acccggaaac acggtgttta tactaatcac cgaacgttat gatggcattt taccaactat
     5761 actttcgcgt acacagttag ttaaaattcc gaaaatagat aatgattcat taataaagta
     5821 tgcctgcgaa aaatatggct tagatatcaa caaagctcgc aatatggttc atctttcggg
     5881 taatgacata acagagttaa tggcactaat gaatcaggaa aacttatccg gacaaatgga
     5941 acattctttt atagagtgga tgcgtatgtg ctataaattt ggaacgaatt ataaagtatt
     6001 atacagttgg attgaagaca tatcaaaact ttcgcgcgat gcacaaaaaa actttttgag
     6061 cttttgtttg ggagctgccc gcgagtgttt aattaccaac atggcaggta taaatcactc
     6121 acgatatact gatgcaactt ttaatggttt tgagcgattc agtaagatga taaatgccga
     6181 taatgttgag ccttttgcaa aaacacttga agaagctatt tatcatattg agcgtaatgc
     6241 caatccaaaa atattatttt tagatttgtc gttcagaatg catcgcattt tacacatgaa
     6301 attttaattg attaatgaca accacaacaa taaataccaa tacaaaaagt acaggatgca
     6361 cttcgtgcgg aacaggcagc tgcaataagc tgaatgttta tgactggctt agtgatatgg
     6421 agtttccacc aggacatatt ccattcaatg tagtagaagt gcagtttaaa ggaacccgta
     6481 aagaatattt cagaaataca gacggacaca tacttaaagc cggagatata gttgccgttg
     6541 aaggaaatcc cggacatgat attggcacag taaccatgac aggtgagttg gttaaacttc
     6601 aacttaaaaa gcgtggcttc gaaagtggtg ctccggagat taaacaactg tacagaccgg
     6661 ccaaacaagg tgacattgaa aagtgggaac aagtaaaaca attagagcat caaaccatgc
     6721 accgtgcaag aaagatagca ttgcaacttg gcttaaaaat gaagcttagt gatgtcgaat
     6781 atcagggtga tggcaagaaa gctacattct tttatactgc cgaagaacgt gttgattttc
     6841 gtgagttgat aaaaaaacta catgaagagt ttaaagttag aatagatatg aggcagattg
     6901 gtctcagaca ggaagcaggg cgtcttggag gaataggttc atgtggaaga gagctttgct
     6961 gctctacctg gatgacaagt tttaaaactg ttggtacttc agcagcgcgc tatcagaatc
     7021 tgtcaatcaa ccctgtaaaa cttgccggac aatgtggaaa attaaaatgc tgtctcaact
     7081 atgagttgga cagttacatg gatgccatta aagacattcc cgaacaaggt actgctattt
     7141 ttactgaagc cggaaaagca ttacacagaa aaactgatat ttttaaacgc atcatgtggt
     7201 atgaacttat tccgaattta aaggatggcg aatactacca acccagtgca gacaagtggg
     7261 tgatgttgcc tgttgacaga gttaaagaaa ttattgcact caacaaggca aaccaaaaac
     7321 cggctgatct gaagagcgga cttcctgtag aagatgaggc agaagaacct gattatgccg
     7381 atatggttgg acaagatagt cttaccagaa tggataaaaa gaaaaagaag aaaaagaaga
     7441 agaaaaagcc ttcgcagcct aatcaacaaa cgaacaatgg ccaacagaaa aagccttctt
     7501 aggtatttaa ttgccggggt tgtaattgta gtgtttgctt cctgcaatca acagtatcat
     7561 tttgaagaaa acaaatccat caaaggcagc aattgggatt tttctgaacc aataagtttt
     7621 gatgttaaca ttgatgatac actcactgcc tataacatgt acattaatgt acgcaatcag
     7681 ggtaactatc gtttcagtaa tttattttta ttcatcaaca cacatttacc acaaggtcag
     7741 catttacgcg atacagtaga aataactttg gctactcctg acggagcttg gctcggtaaa
     7801 ggcattggtg atttatattc caatcgttat ttgtttcgta aaaatttccg attccctcaa
     7861 aagggagatt atcacgttga gttaattcag gccatgcgtg taaatccgct cccgggtatt
     7921 cttgatgcag gtatcagaat agaaaaaaca aaacaataat tttcctcaag tgactgtcaa
     7981 aatttagtat aaaacataaa agccgctttg ttttcaaaac ggctttttga tgttagaatt
     8041 atttatttct tattctacaa tattctgacg tgctttttcc aagcctgatg ccaatcgggt
     8101 taagaatgct ttgtctatct gtttttcgtc cgatggctct ttataaattg ccaacaatgc
     8161 ctcataatct gactttattt tctttacctc atcattatcg gcaaactcac ttaacaattt
     8221 tgtaatgttg tcaagataca aacgttgctc ccataccctt ttatataata tttcattgtc
     8281 ggcatttctg cttgctgtaa gcaacgactt aacagtaaga tattgacatt cgagccagct
     8341 tcctgctaac acctgactgg ctgtttccaa ccttttgtta ctgcgtaaat aagcatccgt
     8401 tgcagcgtat gcatcatcaa tcacacgggt caaagaatca cgattgttgc tgtttgtctc
     8461 aaaacgattt acaaactgat taaatgtttc ttccatgccc aattgtgacg caagtttctt
     8521 tgcagtgttg taatacttaa tggcatctaa tgttctgttg ttaacaacca aatagcccaa
     8581 atcaacacca tagataccgt agttcatggc ttgtttaagt gatgtcatgt atttgtctgc
     8641 attatctgcc gaattgagca atgcagtatt tacttcaagt tttgatttac taaactcatc
     8701 taatacctgc atgggcggag gcaggttagc tatggtgtag gtaaactgaa atgttgccac
     8761 ttcactaacc ggcatggtat ctttagtttc tgccgtctgc tctttgcttt cttcgcctga
     8821 tttattgctg cacgatccgg tgagcaacaa tccggcaata acagtggata aatagatata
     8881 gcgtttgcgc atagttttat tttcagcaaa cgtaacagat ttttttgctt aatgcaaaaa
     8941 atgatgaatc atactttaat tcaaaggttc atcttagggt taaaactcct gaaaaaatag
     9001 gctttgatgg ttttataaac aaactcaaaa accggaagcc ggattatcaa aagataatat
     9061 tggctctgct aaaatcagtc ctttttttat gttagttttt tcaaaacttg tcccgattgc
     9121 ccgggaactt gacgaagaag ggattaacgc agtttcgaaa aatggagata cgaagaacaa
     9181 ctgtttgata tgtgttgcaa ggtttcactc acgatgacga gtttgcctca ccacacaact
     9241 ctttgtttaa ctacgaccga acgtactgtt cagtactggc ggatatcaaa gtccgataac
     9301 tatcgaacct tcttttttct atttctttta atctttatgt tacctgcatg tggcactgtt
     9361 cgttcgaagt tcatcaattt gaagtccgca gagaagtttg ttagaagttc gtaatcgtat
     9421 cgtgtcatgc aggttgttct tcttttgctc cgagtttatc aaattcaatt tgcagttttc
     9481 ctaatgcttc tttttcacct tgcagtttgt caagcggata actaagataa gcgaatgttg
     9541 ctccaatgcc acgaatgatg taactgattg accagattat tctgtcaagg tcttgattta
     9601 tattttgtcg ctgcatgaag tgtgtcatga taccgaaatg ctcatacttt gaatagtaag
     9661 tataaaggtc ataaaccatt gaaaactttt ttgtcaaagg atgagtgtga attcttgtaa
     9721 agatttgttt tggtgacttg aacatctttg
//
LOCUS       DAOQNP010000091         9568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2612_length_9568_cov_11.163776, whole genome shotgun sequence.
ACCESSION   DAOQNP010000091 DAOQNP010000000
VERSION     DAOQNP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2612_length_9568_cov_11.163776"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKY09_10895"
     CDS             complement(<1..649)
                     /locus_tag="PKY09_10895"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNB13404.1"
                     /translation="MKNNFSHIGLAKLCGWFGITRQAYYQNNWEGISTTLEEDLIIQQ
                     VKEIRKNHRRMGTRKLYEMLQPFILEHQIKIGRDALFNMLSANHLLARKRKRRIQTTN
                     SYHWLRKYPNLIREFVPTTPNQLWVSDITYWKINTGEHLYISFITDAYSHKIVGYQVA
                     ETMEAIESIHALQMALSALGAESHLNLIHHSDRGIQYCSHAYVKLLQDKGIKISMT"
     gene            complement(655..1101)
                     /locus_tag="PKY09_10900"
     CDS             complement(655..1101)
                     /locus_tag="PKY09_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13405.1"
                     /translation="MDKKIIQKPQKYFTEAEKHKIIQELISTQCTKVEIWEKYTGEEE
                     EHGQLLRWMRQLGYNTGIKTRRPNIVSNLYPMAQRKIKSDNPDSYREVNKDDESFENL
                     QLKKRIAELEKQLKDAELKAIAFSTMVDIAEKEFKIPIRKKLNTKP"
     gene            complement(1217..1405)
                     /locus_tag="PKY09_10905"
     CDS             complement(1217..1405)
                     /locus_tag="PKY09_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13406.1"
                     /translation="MNAYYNGCDDFGIGLEIFYNIPKEGRYDKYKNLNPDDMSIYLNK
                     TIHYKNYRDFEEAGYIMY"
     gene            complement(1402..2007)
                     /locus_tag="PKY09_10910"
     CDS             complement(1402..2007)
                     /locus_tag="PKY09_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13407.1"
                     /translation="MNNILKILFIVAISTNLSSCKKKEVEVYYRLKFCDTCLTIGGAI
                     EYEKQNNSIELPPMPEEEFYQSIYHKFDPEYSIRLNKGTHEKHIYAFKNFLDVDTIFK
                     LKILKAYIYPFELKEDSLLNRLFLEEIRIKIFNTDSVIDSLYDLPRFTECKYDSLKLF
                     LISLKKSMISILKKSMVNQKGNEFFLMKGRQIFKIIRSLVI"
     gene            complement(2016..2231)
                     /locus_tag="PKY09_10915"
     CDS             complement(2016..2231)
                     /locus_tag="PKY09_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13408.1"
                     /translation="MIILIWSSKEGQIRTEAERTHTVLLDAKGIALTDFASPKINEVR
                     NEGDLKDLKLAKIEKARGLVKEKFGYK"
     gene            2669..2809
                     /locus_tag="PKY09_10920"
     CDS             2669..2809
                     /locus_tag="PKY09_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13409.1"
                     /translation="MKKTIAKKTKKAKKLIAKPAAIVAFLRDLFAEELEVLNRKNIAM
                     HN"
     gene            complement(2820..3272)
                     /locus_tag="PKY09_10925"
     CDS             complement(2820..3272)
                     /locus_tag="PKY09_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNB13410.1"
                     /translation="MVSSDVLNKARRYCTYQERSQQELREKLYSLGLFKKDVEETIAM
                     MVTEGYLNEERFAIAYAGGKFRIKHWGKNKIKQALKLKKVSDYCIRQALKSISDDDYL
                     KVLNEQTELCRKKIKESNTIKKNYKVAQQLMARGFEADEVWKCILKEK"
     gene            complement(3275..4702)
                     /gene="pyk"
                     /locus_tag="PKY09_10930"
     CDS             complement(3275..4702)
                     /gene="pyk"
                     /locus_tag="PKY09_10930"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNB13411.1"
                     /translation="MHHFNKTKIITTLGPATNSKEAIYKLIQAGADVMRINASHGDHT
                     FLKSIVNNVREINEEKQMNIALLLDLQGPKLRVGDIEGGQCELADKQQFVLTTKEMDG
                     NATKATIRYKDFANDVKKGDIILIDDGKLKLEVKQVTAEQDVVTQVINGGVLMSKKGV
                     NLPFTNISIPSITEKDRADIDFAIENNIEWIGLSFTRTAQDILNLKKIIKDCGNAMRV
                     IAKIEKPEAVRNINEIIEVADGIMVARGDLGVEIDLAQVPIIQKKIVKKCRAAGIPVI
                     IATQMMESMITNFRPTRAEANDVGNAVYDGADALMLSAETSVGKFPVETVNSMRKIIQ
                     AIEHDQEIYYRMNQPDKNSKTFISDTLCYHAAELANQSGAKAIAAMTHSGYTAFRISS
                     QRPKAGLFIFTDYKPILNTLNLVWGVRGIYYNKFESTDQTIDDIKNILKEQGYVDAGD
                     VVIHVASTPLKKRSRVNTLKINVVD"
     gene            complement(4705..5130)
                     /locus_tag="PKY09_10935"
     CDS             complement(4705..5130)
                     /locus_tag="PKY09_10935"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNB13412.1"
                     /translation="MAKKVIKFDNSSELDFLLAGIVCPYKDYRLCFELNRALTLSFKR
                     MKDHTLYLGKPGAVAAFSFHKAINRFGEEFYVLGNKSVQATLIPEKPAFDFFMIVKNY
                     GAYFDFNDIRNKIKQIEMVSAVSELRVEELKSADNFIFD"
     gene            5192..5641
                     /locus_tag="PKY09_10940"
     CDS             5192..5641
                     /locus_tag="PKY09_10940"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNB13413.1"
                     /translation="MSIIQQGDDSFMQEALKEARKAFDCDEVPVGAVIVHAGRIIARA
                     HNMTEKLNDVTAHAEMICFTSAAAFLNSKYLTDCVLYVTLEPCVMCAGASFWTQIPQI
                     VFGATDAKRGYQRCKTNLLHPSTKIKGGVLEEACSNLLKEFFRKKRS"
     gene            complement(5638..6027)
                     /locus_tag="PKY09_10945"
     CDS             complement(5638..6027)
                     /locus_tag="PKY09_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNB13414.1"
                     /translation="MANKYHSVLVVDDSEAEQVMIKQIITSEPFCDQLNVYADARKAL
                     DFIKATPVDQLPEIIFLDIVMPEMDGFQFLNEFEKLSESVRQKCKIVLLSSSDSFKDL
                     NRANKCRYVRRFLNKPLTNSMLQAINF"
     gene            complement(6032..6553)
                     /locus_tag="PKY09_10950"
     CDS             complement(6032..6553)
                     /locus_tag="PKY09_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB13415.1"
                     /translation="MIRTVTSIPEGNFHSVDFNEVIRQSIDRLRYTEGIDKVNFDIKV
                     DMKAPFAGNNKLLEVVFNNLISNGIKYRKITGDPSSMRLHVYCDEQKAIINIQDNGLG
                     IAPDEVGKIFNLFQKEKQPEPGRGIGLYMAKEIIEKLGGYIAVESVLNTGTAFTIEIP
                     NKLNTKPLNNYFE"
     gene            complement(6550..6903)
                     /locus_tag="PKY09_10955"
     CDS             complement(6550..6903)
                     /locus_tag="PKY09_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13416.1"
                     /translation="MAEHKTSTSKYENNMFPVIKLDYSGKIIYHNVAAMPILSYWQCK
                     LNAPVNNQVKAIYPEIFNVKTPVPHDTTVQYLEYLFHFSVIPYPEAGYIGIYGHCIEE
                     ALVTATHSTEQQHSK"
     gene            7181..7597
                     /locus_tag="PKY09_10960"
     CDS             7181..7597
                     /locus_tag="PKY09_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNB13417.1"
                     /translation="MPYPEQLCAPMRQELTNVGFEELKTTSDVEASLPNSKGTVLLVI
                     NSVCGCAAGTARPGVTLSLRGDKKPGKLVTVFAGQDVEATAQARKYTLPYPPSSPCIA
                     LFKDGELVHFVERHHIEGRSAEMIAENLKAAYEEFC"
     gene            7710..9248
                     /locus_tag="PKY09_10965"
     CDS             7710..9248
                     /locus_tag="PKY09_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNB13418.1"
                     /translation="MLVKTYGCAVYGINATPITVEVNVDQGTKFFHVGLPDNAVKESH
                     QRIEAALKNTGFKMPGKKVVVNMAPADIRKEGSAYDLTIAMGILSASQQIVAENIEQY
                     IIMGELSLDGSLQPIKGALPIAIEARKHGFKGFFLPQQNAREAAIVDSLDVYGISNIK
                     EVIDFFTGEATLQPVVINTRDEFYSAQIKSEFDFCDVKGQENIKRSLEIAAAGGHNVI
                     LIGPPGAGKTMLAKRLPTILPPLTLHEALETTKIHSVAGKMGKQASLISIRPFRSPHH
                     TISDVALVGGGGIPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEERRVTISRAK
                     FSVEYPASFMLVASMNPCPCGFFNHPEKECVCAPGIVQRYLSKISGPLLDRIDLHVEV
                     TPVSFSELSKKRVSEKSEDIRKRVVEARVIQEERYKEHDGVYCNAQMSSKMLHDICRI
                     SDEGNQLLKTAMEKLGLSARAYDRILKVSRTIADLAASPDIKTEHLAEAIHFRSLDRE
                     GWAG"
     gene            9412..9485
                     /locus_tag="PKY09_10970"
     tRNA            9412..9485
                     /locus_tag="PKY09_10970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9446..9448,aa:Asn,seq:gtt)
BASE COUNT         2835 a         1801 c         1759 g         3173 t
ORIGIN      
        1 cagtcatgct gatttttatg cctttatcct gcaatagctt tacataagca tggctgcaat
       61 actgaatgcc tctgtcgcta tgatggataa ggttcaaatg gctctctgcc cccaaggcag
      121 agagagccat ttgcaaagca tgaatacttt ctatcgcttc cattgtttca gctacctgat
      181 aacctacaat tttatgcgag taagcatcgg taataaagct gatgtaaaga tgttcgcctg
      241 tatttatttt ccaataagta atatcactca cccaaagctg attgggtgta gtaggaacaa
      301 actcacgaat aaggtttgga tactttctca accagtgata ggagttagtc gtttgtattc
      361 ttcgcttacg tttccttgcc aataaatgat tggctgaaag catattgaac agtgcatctc
      421 tgcctatttt tatttgatgt tccaatataa atggttgcag catttcatac agctttcgtg
      481 ttcccatcct gcgatggttt ttacgaatct cttttacctg ttgtattatc aaatcttctt
      541 ccagggtcgt tgaaattcct tcccagttgt tttgataata agcctgtctg gtgataccaa
      601 accatccaca tagcttggct aatcctatgt gtgaaaagtt gtttttcatt gcctctatgg
      661 tttggtattg agcttttttc tgatgggaat tttaaactcc ttttctgcaa tatctaccat
      721 cgtggaaaaa gcaattgctt ttagctcggc atcttttaat tgcttttcta attcggctat
      781 acgctttttt agttgcagat tttcaaaaga ttcatcatct ttattaacct cccgatagct
      841 atcgggatta tcagacttaa tttttcgttg tgccatcgga tataaatttg acacaatgtt
      901 aggtcttctg gttttaattc cggtgttgta gcctaattgc cgcatccagc gaagcaactg
      961 cccatgctct tcttcttcac cagtatattt ttcccaaatc tctaccttgg tacattgggt
     1021 tgaaattaat tcctgaatga ttttgtgttt ctcggcttct gtaaaatact tttgtggctt
     1081 ttgaatgatt tttttgtcca ttttttacac ttttttgtgt aaacctattt caggacaaga
     1141 cacaggtgtt aatcgcatcc tcgtctcgcg cacaccactc cctcctcccg tcagatgagg
     1201 acgagggggg taaaacttaa tacataatat atccagcctc ctcaaaatcg cggtaatttt
     1261 tgtaatgaat agttttgttc aaataaatac tcatatcatc agggttgaga ttcttatatt
     1321 tgtcatacct accctctttt gggatattat aaaatatttc caatccaata ccaaaatcat
     1381 cacatccgtt ataataggca ttcatatcac caatgagcgg ataattttga atatttggcg
     1441 ccctttcata agaaagaatt cgttgccctt ctgattgacc atacttttct ttaaaatact
     1501 tatcatactt tttttcaaag aaattaaaaa cagttttaat gaatcgtatt tacactccgt
     1561 aaatcgcggt aagtcataaa gcgaatcaat aaccgaatca gtattaaaaa tttttatacg
     1621 aatctcttct aaaaagagcc tgtttaacaa agaatcctct tttagttcaa atgggtatat
     1681 gtaggctttt aatattttca atttaaatat agtatcaaca tctaaaaaat ttttaaaggc
     1741 gtaaatatgt ttttcatgag ttcctttatt tagccttatt gaatattcag gatcaaattt
     1801 gtgatatatt gattgataaa actcttcctc tggcatagga ggtagttcaa ttgagttatt
     1861 ttgtttttca tattcaatag ctccgcctat tgttaagcaa gtgtcgcaaa attttagtct
     1921 ataataaacc tctacttctt ttttcttaca acttgacagg ttggtagata tggctactat
     1981 gaaaagaatt tttaaaatat tgttcatgtt ttattctact tatagccaaa cttttcttta
     2041 actaaacctc ttgccttttc aattttagct aatttcaaat cttttaaatc accctcattc
     2101 ctcacttcat ttattttagg cgatgcaaaa tcagttaagg ctattccttt ggcatccaat
     2161 aaaactgtat gtgtgcgttc ggcttcggtt cttatctgtc cttctttgga actccaaatc
     2221 aaaattatca agaatatcct gatcaacaaa actgtaatct acataacggg aaggataatc
     2281 agcttctaaa tatttttgag gagcacgaac gtttgcaaat ctgaaaactt cgttgggata
     2341 tctaaggtct gccatctgat ttggttttta gagtgactga aatgtgaatc aaatttaatt
     2401 tgaaataact ggaatcgtat taaattagta taaataagtt atcaacaata agacatcgtg
     2461 tatagatttt tagattgata gtttgaggta aaaaatttgt gtgctgcatt tttcagctgt
     2521 ataggcacga ttattacttt aggacaaata gaaaataata aattccggaa aatctcgacc
     2581 tgttgatatc cagtggttta tctatttaaa ttttttggta caaaatttga aatatggtaa
     2641 gtaagtataa catataatac tccatattat gaaaaaaacc attgctaaaa aaaccaaaaa
     2701 agcaaagaaa ttaattgcaa aacccgcagc cattgttgca tttcttcgcg atttatttgc
     2761 tgaagaactt gaagtcctta accgcaaaaa catagccatg cacaactaat ctgcatggct
     2821 tatttttctt ttaatataca tttccaaact tcatcagctt caaaaccacg agccattagt
     2881 tgttgcgcaa ccttgtagtt ttttttaata gtattacttt cttttatttt ttttctgcaa
     2941 agttctgttt gttcattcag cactttcaaa tagtcatcat cagatataga ttttaacgct
     3001 tgcctgatac agtagtcaga aacttttttt agtttcaatg cttgtttgat tttatttttc
     3061 ccccagtgtt ttattctgaa ttttcctccg gcataggcaa tagcaaatcg ttcctcgtta
     3121 agataacctt ctgtaaccat cattgcaatg gtttcttcta catctttttt aaatagtccg
     3181 agcgagtaaa gcttttctcg cagctcctgc tgacttcttt cctgataggt gcaatacctg
     3241 cgggctttat tcaacacatc gctgctaacc atccttagtc cactacatta atttttaaag
     3301 tatttactcg cgaacgtttt tttaatggcg tgctggcaac atgtatcacc acatctcctg
     3361 catcaacata accttgctct ttcaaaatgt tcttaatatc atcaatggtc tggtcagtgc
     3421 tttcaaattt attgtaatat atgcctcgca caccccaaac caaattcaag gtgttgagaa
     3481 tgggtttgta gtcagtaaaa ataaaaagcc cggccttggg ccgctgactc gaaattctga
     3541 aagccgtgta tcctgaatgt gtcattgctg caatagcttt tgcacctgat tgattggcta
     3601 actccgcagc atgataacac aaggtgtccg aaataaatgt ttttgaattt ttatcaggtt
     3661 gattcatgcg gtaataaatt tcctgatcgt gttctatagc ctgaataatt tttctcatgg
     3721 aatttaccgt ctcaacagga aactttccta cagaagtttc tgcactcagc atcaaggcat
     3781 cggcaccatc gtaaacagca ttgcccacat cgttggcttc ggcacgtgtg ggtctgaaat
     3841 tggtaatcat actctccatc atttgtgtgg caatgattac aggaattcct gcagcacgac
     3901 atttttttac aatcttcttc tgaataatag gaacctgtgc cagatcaatt tctacaccta
     3961 aatcaccacg ggctaccata ataccatcag caacttcaat aatctcattg atgtttctga
     4021 ctgcttccgg tttttcaatt tttgcaataa ccctcatggc attgccgcag tctttaatga
     4081 ttttttttag attcaatata tcctgagccg ttctggtaaa tgataagcca atccattcaa
     4141 tattattttc aatggcaaag tctatatctg cacggtcttt ttctgttatt gatggaatgc
     4201 tgatattggt aaacggaagg ttaacacctt ttttcgacat cagcacacct ccatttatca
     4261 cttgagtaac aacatcctgt tccgctgtga cttgctttac ctctaacttt aattttccgt
     4321 catctatcag aataatatcg cctttcttta catcgttagc aaaatcttta taacggatag
     4381 tagcctttgt agcattaccg tccatctctt ttgttgtgag aacaaattgc tgcttgtcgg
     4441 caagttcgca ctgcccgcct tcaatatcac cgacacgcaa tttaggacct tgtaaatcga
     4501 gtaagagcgc aatgttcatt tgtttttctt cattgatttc gcggacatta tttacaatag
     4561 acttcaaaaa agtatgatcg ccatgcgaag cattgatacg cataacatcg gcacctgcct
     4621 gaatgagttt gtaaatggct tcttttgaat tggtggccgg acccagggtg gtaataattt
     4681 tagttttatt aaaatgatgc ataatcagtc gaaaataaag ttgtcagccg acttgagctc
     4741 ttcaacacga agctcactca cggcagatac catttctatt tgttttattt tgttgcgaat
     4801 gtcgttaaag tcaaaatagg ctccgtaatt ttttacaatc ataaaaaaat caaaggcagg
     4861 cttttcagga ataagtgtag cctgaacact tttgtttccg agcacataaa actcttcgcc
     4921 aaaacgattt atggctttgt gaaaagaaaa ggctgccact gcacccggct tgccaagata
     4981 caaggtgtga tctttcattc ttttgaaaga aagtgttaat gcacggttta gttcaaaaca
     5041 aaggcgatag tccttatacg gacaaacaat tcctgcaagc agaaagtcaa gctcactgct
     5101 gttatcaaac tttataacct tctttgccaa gagaaacaga ttgtttattt aaaacggata
     5161 ttttcgctgc taaattagat taatttgtag tatgagcata attcagcaag gtgatgattc
     5221 ttttatgcag gaagcattaa aggaagcaag aaaagctttt gattgtgacg aagttcctgt
     5281 aggtgcagta attgttcatg caggacgaat tattgcacgc gcacataata tgactgaaaa
     5341 actgaatgat gttacggcac atgcagaaat gatttgtttt acttcagcag cagcattttt
     5401 aaactcaaaa tacctgaccg attgtgtttt atatgtaacg cttgaacctt gtgtgatgtg
     5461 tgcaggagca tccttctgga cacagatacc tcagatagtt tttggtgcta ctgatgcaaa
     5521 acgcggctat cagcgatgca aaaccaacct gctgcatcct tcaacaaaaa ttaaaggagg
     5581 cgtacttgag gaagcatgca gcaacttact gaaagaattt tttcgtaaaa agagaagcta
     5641 gaagttgata gcctgcagca tggagttggt taatggttta tttaaaaacc ttctcacata
     5701 acggcatttg ttggcacgat tcagatcttt aaaactgtcg gagctcgaca ataaaactat
     5761 cttgcatttc tgacgaaccg attcagatag tttttcgaac tcattcaaaa actgaaagcc
     5821 atccatttca ggcattacaa tgtctaagaa aattatttcg ggcaactgat ctactggtgt
     5881 tgctttaata aaatcaagtg ctttacgggc atcagcataa acattcagct gatcgcaaaa
     5941 aggctccgat gtaatgattt gttttatcat cacctgttcg gcttcactat catctacaac
     6001 taaaacagag tggtatttat tggccataag cttattcaaa atagttattt aatggtttgg
     6061 tgtttaattt atttggtatc tcaatggtga aagctgttcc tgtatttaaa acagattcaa
     6121 cagcaatgta tcctcctaat ttttcaatta tttctttggc catatataag ccaatgcctc
     6181 tgccgggctc gggttgtttt tctttctgaa acagattgaa gatttttcct acttcatcag
     6241 gtgcaatgcc cagtccgtta tcctgaatat tgatgatggc tttttgttca tcacaataaa
     6301 catgtaatcg catggaagat gggtcaccgg taatttttct gtatttaatt ccgtttgaaa
     6361 ttaaattatt gaaaactact tccagcaatt tgttgttgcc agcaaaagga gctttcatat
     6421 ctactttaat gtcaaaattt actttatcaa ttccttctgt atagcgcaac cggtcaattg
     6481 actgacgaat aacttcgtta aagtcaaccg aatgaaaatt gccttcggga attgaagtaa
     6541 ccgttctgat cattttgaat gctgttgctc tgttgaatgt gtcgctgtaa ccaatgcttc
     6601 ttcaatacag tgtccataaa tgcctatata tcctgcttcg ggatatggta ttacagagaa
     6661 gtgaaacaag tattcaagat attgcactgt tgtatcatgt ggcacaggtg ttttgacatt
     6721 aaaaatttca ggataaatag cttttacctg attgtttaca ggagcattta gcttgcattg
     6781 ccaatagctt aatataggca ttgctgcaac attgtgataa ataatttttc cgctgtaatc
     6841 taatttaatt accggaaaca tgttgttttc gtactttgaa gtagaggttt tgtgttctgc
     6901 cattgtggag atttctttaa attaaacagt agtttaacga cagtttttca gaaatgcttc
     6961 actactatga ccggttatgc ttacagatgt aagattttga tcagtttatt tggtttttta
     7021 ttcattaaaa attaaagccc cctcgataaa ggaataaatt caggaatcct gaagtgtaaa
     7081 aaatattgta agaaacctaa acaaagtgct gcaaaatctg ttttcagaac tgaataacaa
     7141 ataagagtat ttatatactt ttgcaaaaaa taatttaaaa atgccgtatc cggaacaatt
     7201 atgcgcaccc atgcgccaag aattaaccaa tgtaggtttt gaggagttaa aaaccacttc
     7261 agacgttgaa gcatcattgc caaacagtaa gggaacagtg ctgttggtta taaactcagt
     7321 atgcggatgt gctgcaggaa cagcacggcc gggagtaact ctttcattaa gaggcgataa
     7381 aaaaccgggt aaattggtta cagtctttgc aggacaagat gttgaagcaa ctgcacaagc
     7441 cagaaaatac accttacctt atcctccttc atcaccttgc attgctttat ttaaggatgg
     7501 tgagttggtt cattttgtag aacgccatca tattgaaggc cgctcggcag aaatgattgc
     7561 agagaatctg aaggccgctt acgaagaatt ttgttaacca catttactgc agaggcaatc
     7621 tttgcagatg ctattttagg tttttaaagt tattaatggc tatttttgtt tatcgaatag
     7681 aaaaacagaa ttagccatta ttattttata tgcttgttaa aacttacggc tgtgcagtct
     7741 atggaatcaa tgccacacca ataacggtag aagtaaatgt tgatcaggga accaaatttt
     7801 ttcatgttgg cttgcccgat aatgctgtaa aagaaagcca tcagcgcatt gaagctgcat
     7861 tgaaaaatac aggttttaaa atgcccggaa aaaaagtcgt agtaaacatg gcccctgcag
     7921 acattcgcaa agaaggctcg gcttatgatt taactattgc tatgggaatt ctttctgcaa
     7981 gccagcagat tgttgccgaa aatattgagc aatatattat catgggcgaa ctttcactcg
     8041 atggcagcct gcaacccata aaaggagcat tgccaatagc aattgaagcg cgcaaacatg
     8101 ggtttaaagg tttttttctg ccacaacaaa atgcaagaga ggcagctatt gttgattcgc
     8161 ttgatgtgta tggtatcagt aatatcaaag aggttataga ctttttcacc ggtgaagcta
     8221 ctttacagcc tgttgtgatt aatacacgcg atgagtttta ttctgcacaa ataaaaagtg
     8281 agtttgattt ctgtgatgtt aaaggtcagg aaaacattaa acgttcgctg gaaattgcag
     8341 cagcaggagg gcacaatgta atattgattg gtccgcccgg tgcaggcaaa accatgctgg
     8401 caaagcggct tccaaccata ttaccgccat tgacattgca tgaggcattg gaaaccacaa
     8461 aaattcattc tgttgccggc aaaatgggca agcaggcatc attaatttcc attcgtccat
     8521 tcagatctcc acatcatacc attagcgatg ttgcactggt tggcgggggt ggaattccac
     8581 agcccggaga aatatcgtta gcgcataatg gcgttttgtt tttagacgaa ttgcctgagt
     8641 ttaagcgaac cgtacttgaa gtgatgcgac agccgttgga ggagcgaaga gtcaccattt
     8701 caagagcaaa attttctgta gaatatcctg ccagttttat gcttgtggct tctatgaacc
     8761 cttgcccctg tggttttttt aatcatccag aaaaagaatg tgtttgtgct ccgggtattg
     8821 ttcagcgcta tctcagtaaa atttcagggc cattgctcga caggattgac ctgcatgttg
     8881 aagttacacc ggtttctttt tcagagctat cgaaaaagcg tgtctccgaa aagagcgaag
     8941 acatcagaaa aagagtcgtt gaggcacggg taattcagga ggaaagatat aaagaacatg
     9001 acggtgttta ctgtaatgca cagatgagca gtaagatgtt acacgacatc tgccgtattt
     9061 ctgatgaggg taatcaactg ctaaagactg caatggagaa attaggtctt tcggcaaggg
     9121 cctacgacag aatattaaaa gtatctcgaa cgatagccga tttagctgcc agccctgata
     9181 taaaaaccga gcatcttgca gaggcaatac acttcagaag tttagatcgc gaaggctggg
     9241 caggataaaa aaacgggtta aaattttttt ttaaccaaaa aaagctattt ttgccgtccc
     9301 tttacaaacg tgttcgtgat tacacgtgct ttagttccga ttgttttcgg aatggttaga
     9361 gttaagaaaa acatttttct gtcttagggc agaaattctt aagaggtaaa ttcctcctta
     9421 gctcagctgg ttagagcaca tgactgttaa tcatggggtc cctggttcga gcccaggagg
     9481 gggagcaaag ccgaaggttt taccttcggc ttttttaatt aacatattga gaagatgaaa
     9541 tctgttgttt tcattgataa tctgatta
//
LOCUS       DAOQNP010000092         9246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2746_length_9246_cov_7.145686, whole genome shotgun sequence.
ACCESSION   DAOQNP010000092 DAOQNP010000000
VERSION     DAOQNP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2746_length_9246_cov_7.145686"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKY09_10975"
     CDS             <1..350
                     /locus_tag="PKY09_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13419.1"
                     /translation="GRSVPNHPNYYLGRLVGSPCDTLTNINDLAEHDFKFSISPNPSN
                     GTFKIMYLLPQNNKGTFEVFDITGKKVFSYSLPPWSTLQNFDLSFLGNGVYHCSITSN
                     NQRVNKKLIILNE"
     gene            complement(481..1293)
                     /locus_tag="PKY09_10980"
     CDS             complement(481..1293)
                     /locus_tag="PKY09_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNB13420.1"
                     /translation="MYNLLKGKRGIIFGALDENSIAWKTAQRAHQEGAMFTLTNAPVA
                     MRMGEINKLAAECNAQVIPADATSIEDLENLFTKSMEILGGKLDFVLHSIGMSPNVRK
                     GKHYTALDYDFFHKTLDVSALSLHKTLQTAMKLDALNEWSSVVALTYIAAQRVFPHYN
                     DMGDAKSLLESITRSFGYHYGMAKKVRVNTISQSPTATTAGSGIKGFNAFYNYADVMS
                     PLGNASADECADYTVMMFSDFTRKVTMQNLFHDGGFSYTGVSLNAMEKFMDE"
     gene            complement(1342..3252)
                     /locus_tag="PKY09_10985"
     CDS             complement(1342..3252)
                     /locus_tag="PKY09_10985"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNB13421.1"
                     /translation="MRFKKSTWVTASVILIIVALLQWSFITVNENEYQQQKLEAYLSK
                     SALDFTNELEKLKALAGSEKANSHQLVEQFKACRISYKKLEGFTGYYFAYTERDLNGP
                     PIAMVEEEPQSKYVRPPHGLQVIEEIITEKPLNKRRLLDEVNHTLGLAAKLSLSFKTL
                     HPYDYQLFEIMQLRLVKIYTLGINNFDCQVIKTGISETKAALLSISNILNISYRNADF
                     SMVNSSIDSAVAFLDRQQIKVNQGAEFDYFTFYRNYVVKVNQSLINARKSILKFDPQN
                     IRALNLDAATLFEKEAYNTYFFTKLQRSINGDMLPYLGKLLFFDPILSVNNSVSCASC
                     HKPNMAYTDGLPKSYGFLKNDTLPRNTPTILNAALQRNLFMDASVLVLEDQAKTVVQN
                     EREMHGDFKVVVAKLNQSSAYKKIFSQAFAGTKDTAISEEGILKALAEFQRTQLSFDS
                     PFDKAMRGEGTVAQPVIDGYNIFMGKAKCGHCHFAGLFNGTTPPQYRESEFDIIGVPS
                     SNKRPYKLDGDLGRYNVFKNDELKYAFKTTTVRNIAVTAPYMHNGVYKTLEEVVDFYN
                     KGGGAGLGIHVPDQTLPDDKLKLTKDEKKKLVLFLQALTDTSAVVHFSGQLPAIDDSA
                     SPLNKRIAGGTD"
     gene            3483..5297
                     /locus_tag="PKY09_10990"
     CDS             3483..5297
                     /locus_tag="PKY09_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNB13422.1"
                     /translation="MNYLSVEDISKSFGDRVLFNNISFGIAKGQKLALVARNGTGKTS
                     LLRILAGLDVPDEGTVTFRNQLKVSYLTQENTLPQLATVADCLTANTHISDALLNEKP
                     HYELTEEEQSHLGKIKSVAGKLGVPFLHQKVNELSGGQQKRLALSKVLIDEADFIIMD
                     EPTNHLDLDMIEWLESFLASTNITMLLVTHDRYFLDEICDEILELEDGKLYRHKGNYS
                     YYLEKKAERDEMESANLQRSKNLYRTELDWMRKQPRARGTKSKSRIDAFHVLEEKTKK
                     KNEHGNVALDINISRIGSKILEIHRISKSYGDLKLIDNFDYSLRKGEKIGIVGSNGAG
                     KSTLLNIIMGKEPCDKGKIVMGETIVPGYFEQQSKNISDQKRVIEVIREIADFIPMSG
                     GRKISASQLLERFLFDPSMHFQYVEKLSGGEKRRLYLLTVLMKNPNLLILDEPTNDLD
                     IQTLSVLEDYLASFEGCVLIVTHDRFFMDRVADHIWAFEGDGEISVLNGNYYDYRQKL
                     KEQKREQKKEATVVRGKPEEAASNKKRKLSFKEKQEYEKLESEILLLEEEKTMLEQQL
                     GESDLTHEKLMEVSNRFQHVIHEIDAKTVRWMELAELN"
     gene            complement(5302..6339)
                     /locus_tag="PKY09_10995"
     CDS             complement(5302..6339)
                     /locus_tag="PKY09_10995"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNB13423.1"
                     /translation="MGVYVKSSACISARNTFGETDLSEHGLQTEKLMAREPVYSMIAP
                     AVLRRMGKAVRMTCGAVSKLNLESNPVNGIVIGTANGGMEDCIRFLNQIVQYDEGTLT
                     PTNFVQSTPNAIASTIGMNLQCHGYNITHVHRGNAFENALLDALMYLDESKNTSLLLG
                     GIDEISDYNYNIERLGGWYKKEIPVNGLYETKTEGSIAGEGCAVFCVSNQPQNALFEI
                     VDVSTMHSTDVDAVQSGIKQFCSKHNVNFSETMLLSGHNGDSRNETLHHAVENIFNQS
                     TVIRYKHFFGEFPTVAALALWLIGHAATNSGILPDHFYLCKTEKKIKNYIICNHYKGY
                     QHGFILARSCS"
     gene            6522..7256
                     /gene="gldF"
                     /locus_tag="PKY09_11000"
     CDS             6522..7256
                     /gene="gldF"
                     /locus_tag="PKY09_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNB13424.1"
                     /translation="MYSLFKKEINSFLNSLIGYISVAVFLIVIGLFLWVFPGNDLNIA
                     ESGYAAIDSLFNITPWVYMFLIPAITMRMFSEEKRTGTLEILLTKPLTEFQIVLAKYL
                     SGLVLVIFSLLPTLVYVITVYQYAAPVGNIDLGGIAGSYIGLFFLASGFVAIGVFASS
                     LADNQLVAFIIAMFLCLLCYTGFQSVATILATGVVANLIDQLGIVAHYNSMSRGVIDS
                     RDVIYFISLTALFLVFTRLKLESRKW"
     gene            7258..8943
                     /gene="gldG"
                     /locus_tag="PKY09_11005"
     CDS             7258..8943
                     /gene="gldG"
                     /locus_tag="PKY09_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HNB13425.1"
                     /translation="MKKKIRSANLYQFLLLLLVIVGLNTLGKFLFWRFDLTSEKRFTL
                     SSTTKEKLKNLQDVVYIKVYLTGDLPAGFQRLSSATRDMLMEMKSYAGQNLEFDFIDP
                     SALADSKQRNELYNQLADKGLQPTNISERNAEGTSERILFPGAILNYLSKEQPLLLLK
                     DRMGASAEEMVNTSIQNLEYEIINAISKISTRKPATIGILHGQGELDKPYLADFYKSV
                     SASYPTHYVQIKNQLNALKDYDCLVIAKPDSAFDEKDKFIIDQFIMRGGKVLWLLDMM
                     SADMDSLARKNEFIAIPKELNLDDMLFRYGVRLNKDLVMDLQSVPIPILTGYVGNRPQ
                     NSLLPWFYYPLVTPTSKHPIVNNLNAIRFAFAGSIDTIVSAHINKTILLQSSRYCKTV
                     QSPAVVDLNILRKEPDEKEYRNPPVNLAILLEGSFRSNFANRIPYQIATDSTIGFKAE
                     GKPTSMIVISDGDVIRNDYKRSSNTVYPLGLDRYTGQFYGNKNLLLNCIDYLCDNSGL
                     MTLRAKEFKLRLLDKTRFNPDKKMLQIVNVALPVLLIILFAMIKLLMRKKKFS"
     gene            8951..>9246
                     /locus_tag="PKY09_11010"
     CDS             8951..>9246
                     /locus_tag="PKY09_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13426.1"
                     /translation="MNKNRILLLIVVLLLSVTGYYYFKNSDSTIKKELRDFAVADTSS
                     IDKIFLADKKGNKALLERKSPALWTINGKYPARQDAINFLLKTIKQIEVRSPVG"
BASE COUNT         2831 a         1700 c         1866 g         2849 t
ORIGIN      
        1 atggaagaag tgtccccaac caccctaact attacttagg gcggttggtt ggcagtccgt
       61 gcgatacttt aacaaacatc aatgatttag cagagcatga ttttaaattt tcaatctccc
      121 ccaacccaag caatggcact ttcaaaatca tgtacttgtt gccgcagaat aacaagggca
      181 cttttgaggt gtttgatata acaggcaaaa aagttttttc atatagctta ccgccatgga
      241 gtacacttca aaattttgat ttgagttttt tgggtaatgg ggtttatcat tgcagcatca
      301 ccagcaacaa ccagcgtgtc aacaaaaaat tgatcattct taacgagtag taccatgctg
      361 tgcgcaggcg atccaacaaa ggtgcgaatg tattaccttc tttaatccta aacaataaac
      421 tgttatcata attctgaaag aaatctaaca taacataacc caagcaccaa caacaaaaac
      481 ctactcatcc ataaattttt ccattgcatt gaggctgaca cccgtatagg agaagccacc
      541 atcgtgaaag aggttttgca tggttacctt gcgtgtgaaa tccgaaaaca tcataaccgt
      601 gtagtctgca cattcatcgg cagaggcatt gccaagtggc gacatcacat cggcatagtt
      661 atagaaggca ttgaaccctt taataccact gcctgcagtt gttgcagtcg gtgactgaga
      721 aatggtgttt actctgactt tttttgccat gccataatga taaccaaagc tgcgtgttat
      781 gctttctaaa agtgattttg catcgcccat atcattatag tgcgggaaaa cacgttgtgc
      841 tgcaatatag gtaagtgcca ctacactact ccactcgttt agggcatcga gtttcatggc
      901 tgtctgcaat gttttgtgca ggcttaaggc agaaacatcc aatgtcttat gaaaaaaatc
      961 gtaatcgagt gctgtgtaat gtttgccttt acggacattg ggcgacatgc cgatgctgtg
     1021 taaaacaaaa tctaatttgc cacctaaaat ttccattgat ttggtaaaca ggttttcgag
     1081 gtcttcaata gaagtggcat ctgcagggat aacttgtgca ttgcactcgg cagcgagttt
     1141 gtttatttca cccatacgca ttgcaacagg agcgtttgtt aatgtaaaca ttgctccttc
     1201 ctgatgtgca cgttgtgccg ttttccatgc aatagaattt tcgtctaatg ctccaaaaat
     1261 gattccccgt tttcctttta acaaattgta cataagtatt gttgttttgc ggctaaagta
     1321 atagaaattg atgaaacaac attaatctgt gccaccagca atgcgtttat tcagtggcga
     1381 ggcactatcg tcaatggcag gcaactgtcc actaaagtgc acaacggcag aagtatctgt
     1441 tagcgcttgc agaaaaagaa ctaatttttt cttttcgtcc ttagtaagtt ttaatttatc
     1501 atcgggcaag gtttgatcag gaacatgaat gcccaggcct gccccaccgc ctttgttata
     1561 aaaatcaact acttcttcaa gtgttttata aactccgttg tgcatgtagg gtgctgtcac
     1621 agcaatgttt ctgacagtag ttgttttgaa tgcatattta agctcgtcat tcttaaaaac
     1681 attgtagcgt ccaaggtcgc catctaattt ataaggacgc ttgtttgatg atggaacacc
     1741 aataatatca aactcacttt ctctgtactg tggtggtgta gttccgttaa agagtcctgc
     1801 aaaatggcag tgtccgcatt tggctttgcc cataaaaata ttgtacccat caatgacagg
     1861 ttgtgccact gtgccttcac cacgcatggc tttatcgaaa ggcgaatcga acgacagttg
     1921 tgtgcgctga aattctgcca aggcttttag aataccttct tcgctgatgg cagtatcttt
     1981 tgtacctgca aatgcctgac tgaatatttt tttgtaggct gaagactgat tgagtttggc
     2041 tactacaact ttaaaatcgc catgcatctc tcgttcattc tgaacaacag tttttgcctg
     2101 atcttcaaga accaatacac tggcatccat aaacagattg cgctgcaatg cagcatttag
     2161 aatggtgggt gtgttgcgtg gcaacgtgtc gttctttaaa aaaccatagg acttaggtaa
     2221 gccatctgta taagccatat tgggtttatg acacgaagca catgacacag aattgttgac
     2281 agaaagaatg gggtcgaaaa ataaaagttt gccgagataa ggtaacatat ctccgttgat
     2341 gctgcgttgc agtttagtaa agaaataggt gttgtatgct tctttttcaa acaaggttgc
     2401 tgcatcaaga tttaaggctc tgatattttg tggatcgaat tttaaaatgc tttttctggc
     2461 attgatgagt gattggttta ccttcacaac atagttgcga tagaaagtaa aatagtcgaa
     2521 ctcagcaccc tggtttactt ttatttgctg acggtcgaga aatgctacgg cagagtcaat
     2581 gctgctgttg accattgaga aatcggcatt gcgataagag atatttaaaa tattgctgat
     2641 agaaagcagt gcagcttttg tttcagaaat tccggttttt ataacctgac agtcgaaatt
     2701 attaatgcca agtgtgtaaa tttttacaag tcgcagttgc ataatttcaa acaactgata
     2761 gtcgtaggga tgcaaggttt tgaacgacag tgacaatttt gctgcaagtc cgagtgtgtg
     2821 attgacttca tcgagcaatc gccttttgtt taaaggcttt tcggtaataa tttcttcaat
     2881 tacctgtaag ccatgtggcg ggcgtacata cttgctttgc ggttcttctt caaccatggc
     2941 tattggcggg ccgttaaggt cgcgttctgt ataggcaaaa taataccctg tgaagccttc
     3001 gagtttttta taggaaatac gacaggcttt aaattgctca accaactgat gtgagtttgc
     3061 tttttcgctg cctgcaagag ctttgagttt ttcaagttcg ttagtgaaat caagagcaga
     3121 ttttgagagg taagcctcta gtttctgttg ctgatattca ttttcattta ctgttataaa
     3181 cgaccactgc agcaatgcta ctataatgag aattactgat gcggttaccc atgtactttt
     3241 cttaaatcgc atctttcaaa aatgacaaat aactatatat gtactacgct gagatgaaat
     3301 aattattaac gcaataatga ttatatttca tcaatgtaca aatgacttat gtcaataata
     3361 tttattttat gaagtcaggt atcacaaaaa atgtaatcaa aaatcataca ttttaaaaac
     3421 agtacaaact ataatttatt agcacaataa ctgttacttt cgcgcttcaa tcagaaaaat
     3481 aggtgaatta tttatcggta gaagatatca gcaagagctt tggtgacaga gtattgttca
     3541 acaacatatc gttcggcatt gccaaagggc agaaactggc attggtagca agaaatggta
     3601 cgggcaaaac atcattactc agaattttag ccggacttga tgtccctgat gaaggtactg
     3661 ttactttcag aaatcaattg aaggtgagtt atcttacaca ggaaaataca ttgccgcagt
     3721 tggctacagt tgcagattgt ctgacagcga acacacacat ttctgatgca ttgctgaatg
     3781 aaaaaccgca ctatgaatta actgaggagg agcaatcaca tttgggtaag ataaagtctg
     3841 ttgccggaaa gttgggtgtt ccgtttttgc atcagaaggt gaacgaactt tccggtgggc
     3901 agcaaaaaag attggcatta tccaaagtgt tgattgatga ggccgacttt ataattatgg
     3961 atgagcctac caaccacttg gacttagaca tgattgaatg gcttgaatcg tttttagcat
     4021 caacaaacat aacgatgttg ctggttacac acgaccgtta ttttttagat gaaatatgcg
     4081 atgaaattct ggaacttgaa gacggaaaac tatacagaca taaaggaaac tacagctatt
     4141 atttagagaa gaaagctgag cgtgatgaaa tggagtcggc caatttgcag cgttcaaaaa
     4201 atctttaccg tacagaattg gactggatgc gtaaacagcc tcgggcacgc ggcacaaaat
     4261 ctaaatcgcg gatagatgca tttcatgtac ttgaggaaaa aaccaaaaag aaaaatgagc
     4321 acggtaatgt agctctcgat atcaacatat cgagaattgg aagtaaaatt cttgaaatac
     4381 accgcatcag caaatcgtat ggcgacctta aactgattga caactttgac tactcgttac
     4441 gaaaaggcga aaagattgga attgttggca gcaatggtgc aggtaaaagt acattgctta
     4501 atatcattat gggaaaagag ccttgcgata aaggtaagat tgtgatgggt gaaaccattg
     4561 tgcccggata ttttgagcag caatcgaaaa atatttctga ccagaagcga gttattgaag
     4621 tgattcgtga aattgctgat tttattccaa tgagcggtgg gcgaaaaatt tctgcatcgc
     4681 agttgcttga gcgctttctg tttgaccctt ccatgcattt tcagtatgta gaaaaattga
     4741 gtggtggaga aaaaagaaga ctgtatttgc ttactgtgct gatgaaaaac cctaatcttc
     4801 tgatattaga tgagccaacg aacgatttag acatacagac actctctgtg cttgaagact
     4861 atttagcatc ttttgaagga tgtgttttaa ttgttacgca cgatcgtttt tttatggaca
     4921 gagttgcaga ccacatttgg gcatttgaag gtgatggaga aattagtgtt ctgaatggca
     4981 attactatga ctacaggcaa aagctgaagg agcaaaaaag agagcagaaa aaagaagcaa
     5041 cagtagtgag aggcaaaccc gaagaagcag catcgaacaa aaagcgtaaa ctgtcattca
     5101 aagaaaaaca ggaatacgag aagttagaga gtgaaatttt gctgcttgaa gaagaaaaga
     5161 ccatgctgga acaacagtta ggagaatcag accttacaca tgaaaagtta atggaagtga
     5221 gcaacagatt tcagcatgtg attcatgaaa ttgatgcaaa gaccgtgcgc tggatggaac
     5281 ttgcagagct taactgacca atcatgagca tgatcttgcc aggataaatc catgctgata
     5341 gcctttgtaa tgattgcaga taatatagtt ttttattttt ttctctgtct tacataaata
     5401 gaaatggtcg ggcaatatac ctgagtttgt tgcagcatga ccaatgagcc aaagtgcaag
     5461 tgcagccact gttggaaatt caccgaaaaa atgtttgtat ctgatgactg ttgattgatt
     5521 aaatatattt tcaacagcat gatgcaatgt ttcatttcta ctatcgccat tgtgacctga
     5581 caacaacatt gtttctgaaa aatttacatt atgcttactg caaaattgtt ttattcccga
     5641 ttgaacagca tctacatcag tactgtgcat agtgctgacg tcaacaattt caaataaggc
     5701 attttgcggt tgattactta cacaaaacac agcgcaacct tcaccggcaa tgcttccctc
     5761 agtttttgtt tcatacaaac cgttgacagg aatttctttc ttataccatc cccccaggcg
     5821 ttctatattg taattatagt ccgatatttc atcaatgcca ccaagcagta aagatgtatt
     5881 tttactttcg tctaaataca tcaaggcatc gagcaaagca ttttcaaaag cattgccacg
     5941 gtgaacatgc gttatgttgt agccatgaca ttgaagattc attccaatag ttgaagcaat
     6001 ggcattgggt gtgctctgca caaaatttgt tggtgttaaa gtgccttcat catattgcac
     6061 aatctgattc agaaaacgaa tacagtcttc catgccacca tttgcagtgc ctataacaat
     6121 tccatttaca ggattgctct ctaaattgag tttgctcaca gcaccacagg tcattctgac
     6181 ggcctttccc attcttcgca gaacggcagg tgcaatcatg ctgtaaacag gctcacgtgc
     6241 cataagtttt tctgtctgca ggccatgttc agaaagatca gtttcgccaa aagtatttct
     6301 ggcactgatg catgctgaag atttaacgta aacacccata attttgaaaa ggcaagatta
     6361 agaaatttga cttcacagga aataacttat gctgtcaaat tatttaatta acaattttgt
     6421 cgttataagc ttccttaatc tttcataatc agcagctgcc tatatgcaac gaaactgtat
     6481 ttttggcagt cttaatttca gcattgctgc tttaaattat tatgtattca cttttcaaaa
     6541 aagagataaa cagttttctt aattcgctta taggatatat atctgtagca gtatttttaa
     6601 ttgtgattgg attatttctt tgggtgtttc cgggcaacga cctcaatatt gcagagagcg
     6661 gctatgctgc catagatagt ttattcaaca ttacaccatg ggtttatatg tttttgattc
     6721 ctgccataac catgcgcatg ttttcagaag aaaaacgtac tggaacctta gagatattgc
     6781 ttaccaaacc gttaacagaa tttcagattg tattggcaaa atatttatca ggattggtat
     6841 tggtgatatt ttcattgtta ccaacattgg tttatgtgat tacagtttat caatatgccg
     6901 cacctgttgg caatatagat ttgggaggta ttgcaggtag ttatataggt ttgttttttc
     6961 tggcttcagg atttgttgcc attggtgtct tcgcatcttc attagcggat aatcaattgg
     7021 tggcatttat catagctatg tttttatgtc tgttatgcta taccggcttt cagtctgttg
     7081 caaccatact tgccacaggt gttgttgcaa acctgattga tcagcttggt attgtggcac
     7141 attacaattc tatgagcaga ggtgttattg actcgcgtga tgtaatttat tttatcagcc
     7201 ttacagcatt atttttagtg tttacccgtt tgaaacttga aagcaggaaa tggtgatatg
     7261 aagaagaaga tacgcagtgc caacctatat cagtttttgc ttttactctt agtaattgta
     7321 ggtctcaata cattaggcaa atttttattc tggcgctttg accttacttc tgaaaaacgc
     7381 tttacccttt catctacaac caaagaaaag ttaaaaaacc tccaagatgt tgtttatatt
     7441 aaagtttatc tgacaggtga cttacctgca ggttttcaga ggctaagttc tgcaacacgc
     7501 gacatgttga tggaaatgaa aagttatgca ggtcaaaatc tagagtttga ttttattgat
     7561 ccatcggcat tggctgacag taaacaacgc aacgaacttt ataatcaact tgcagacaaa
     7621 ggacttcaac caaccaatat ttctgagcga aatgctgaag gcacatcaga aagaattttg
     7681 tttcctggag ccatacttaa ctatctttca aaagagcaac ccttgttact tttaaaagac
     7741 agaatgggtg catcagcaga agagatggtg aatacttcaa ttcagaatct ggaatatgaa
     7801 attatcaatg ccatttctaa aatttctacc agaaaacctg ccaccatagg catattgcat
     7861 ggtcagggag agttggacaa accttatctg gctgacttct ataaatctgt ttctgcctcc
     7921 taccctacgc attatgtgca aataaaaaat cagctcaatg cattaaagga ttatgattgc
     7981 ttagtgatag caaagcctga ctctgcattt gatgaaaaag ataagtttat aattgatcag
     8041 tttattatgc gtggcggcaa ggtgctttgg ttacttgata tgatgagtgc tgatatggat
     8101 agcttggcaa gaaagaatga atttatcgct attccaaaag agctaaatct tgatgacatg
     8161 ttgtttcgtt atggtgtaag gctgaataaa gacttggtga tggatctgca gtcagtgccc
     8221 attcctatac tgacaggata cgtgggtaac cgtccgcaga attcgttgtt gccctggttt
     8281 tattatccat tggtgacacc tacatccaaa catccgattg taaataatct aaatgccatc
     8341 cgctttgcat ttgccggaag cattgacacc attgtttcag cgcacattaa taaaactatt
     8401 ttattacaga gttcgcgcta ctgtaagact gttcaatcac cggcagtagt agatttgaat
     8461 atcctcagaa aagagcccga tgaaaaggaa tatcgcaacc cacctgtcaa tcttgccatt
     8521 ttgcttgaag gttctttccg ctctaacttt gcaaaccgta ttccatatca gattgcaaca
     8581 gacagtacta tcggttttaa ggcagagggg aaacctacaa gcatgattgt gatttctgat
     8641 ggtgatgtta tccgcaatga ttataaacgt agcagcaata cggtttatcc acttgggctg
     8701 gatcgttata ccggtcagtt ttatggaaat aaaaatctgc tgttaaactg cattgattat
     8761 ttgtgcgata attccggatt gatgactctt agagccaaag aatttaagct tcgcctttta
     8821 gataaaacac gttttaaccc tgataaaaaa atgctgcaga tagtaaatgt agcattacct
     8881 gttcttttaa taattctttt cgcaatgatt aagttattga tgcggaaaaa gaaattttcc
     8941 tgatagatta atgaataaaa accgaatttt acttctcatc gttgtattgt tactttctgt
     9001 aacaggatat tattatttca aaaacagcga tagtacaatc aaaaaagaac tgcgcgattt
     9061 tgcagttgca gatacttctt ctattgacaa aatttttctg gctgataaaa agggaaacaa
     9121 agcattgctg gaaagaaaaa gcccggcact ttggaccatt aacggtaaat atccggcccg
     9181 tcaggatgct attaacttct tactcaaaac aataaaacaa attgaagtgc gcagccccgt
     9241 tgggaa
//
LOCUS       DAOQNP010000093         8984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2862_length_8984_cov_8.592676, whole genome shotgun sequence.
ACCESSION   DAOQNP010000093 DAOQNP010000000
VERSION     DAOQNP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_8984_cov_8.592676"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKY09_11015"
     CDS             <1..574
                     /locus_tag="PKY09_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNB13427.1"
                     /translation="VPVFIRLANDQSMLEMALVENIQRENLNAIEIAISYKRLIDECK
                     LTQEDLGLRVGKDRTTVTNYLRLLKLPPQIQTALRDDKISMGHARAIISIGDPVMQLK
                     VFNEILENDLSVRSVEDIVRGVSKNEKKQPSISEKFIKGIEYKHIQKRLEDKFETKIE
                     VKHRKNGSGQIMFTYFSTDDLNRLLDILDA"
     gene            587..1183
                     /locus_tag="PKY09_11020"
     CDS             587..1183
                     /locus_tag="PKY09_11020"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNB13428.1"
                     /translation="MRLRLLVVLFFTVYCVNAQQDTMIFKTRTNIVTQADTLKPAADL
                     SQKVNKHSPHKATIYSAILPGLGQAYNKKYWKIPVIYGLTGVLTYFAVDNNKEYIIYK
                     DAYKWRLDDNSATVDKFEGIYSDEDLRILKNYYRRNRDLSFIGMGVVYLLNVVDAAVD
                     AHLFYFDVSDDLSLKVQPGIQPMRHSAFAGVKLSLQIR"
     gene            1187..1900
                     /gene="dapB"
                     /locus_tag="PKY09_11025"
     CDS             1187..1900
                     /gene="dapB"
                     /locus_tag="PKY09_11025"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNB13429.1"
                     /translation="MKIALLGYGKMGKEIEIAGNERGHQIVLKINSENFNQLNKGSLQ
                     TADVAIEFSRPEIAVDNAKLCIDAHIPVIIGTTGWYEHKTVLEQYCIQNNGTAIYASN
                     FSTGVNVFFIINEILSRLMSPMKYDAWIEEVHHIHKKDIPSGTAITLANDILKYNKQY
                     SGWAINDEAGKLPIYAQRIDEVTGFHSVSFRNEVDTIEITHNAFSRKGFALGAIKAAE
                     MALTKKGFYEYKNLLIEQP"
     gene            1905..2546
                     /gene="nth"
                     /locus_tag="PKY09_11030"
     CDS             1905..2546
                     /gene="nth"
                     /locus_tag="PKY09_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNB13430.1"
                     /translation="MLIKKRYETVISYFRKNMPVAETELVYKNPYQLLVAVILSAQCT
                     DKRVNMVTPELFKAFPTAKKLSETEPEEVFEYIKSISYPNNKAKHLVGMARMLLEKFE
                     GTVPSELDNLIQLPGVGRKTANVISSVVFNKAAMAVDTHVFRVAARLGLTRNAKTPLA
                     AEKQLVKHIPEELIATAHHWLILHGRYVCTARNPKCNICGLKEVCVYYKKHPI"
     gene            complement(2543..3157)
                     /locus_tag="PKY09_11035"
     CDS             complement(2543..3157)
                     /locus_tag="PKY09_11035"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNB13431.1"
                     /translation="MLIEINENNIDDRKIKKVVECINNDGIVIIPTDTVYALACSIYS
                     LPAIEKVSRFKGVKPEKANLSFLCNDLKNIAEYTKPFDRSVYKLLNRSLPGPFTFILE
                     ASTKVPGIFRARKKNIGIRIPDQKVTLAIIQQLGHPLMSASLHDINDPITDYLTDPDE
                     IYERFSEMVDMIVDCGAGGNVPSTIVDCTADEIAVLREGKGVLS"
     gene            complement(3199..4257)
                     /locus_tag="PKY09_11040"
     CDS             complement(3199..4257)
                     /locus_tag="PKY09_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13432.1"
                     /translation="MKRFKCLLILLPFCLNLSLAQDVDTIENKHLTNIKQLTFGGDNA
                     EAYFSFDNNKLAFQSNNKAWGVECDQIFLLDLNNWKSDSKPPLISTGKGRTTCSYFMP
                     GNKTILYASTHLGGDKCPETGDLRRGGKYLWPIFNTYDIFVADLNGKVVKQLTNAPGY
                     DAEATVSPDGKKIVFTSDRTGDLELWTMDIDGSNQKQITFGLGYDGGAFFSPDSKQLV
                     FRSSRPKTPEEIQEYKQLLSEGLVAPTNMEIYTCNVDGSNLKQITHLGKANWAPFFHP
                     SGKKILFSSNHESKSGFNFQLYMINTDGTGLVQITDQSVFNAFPMFSPDGKKLIFSSN
                     RNNGGTHDTNLFIADWKD"
     gene            4439..6100
                     /locus_tag="PKY09_11045"
     CDS             4439..6100
                     /locus_tag="PKY09_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13433.1"
                     /translation="MKRILSVLLIILIAHASQAQNVGIGTNVPMQKLDVIGKIRSSTL
                     TGVGNRFVMADPTGTLNVSSSGLLDSTAWLTLGNSSIDTTKNFLGTLTNTHILFKTNG
                     NAAANERMRIRSGGEIVVNNTSVGNNNGDVFSAYATGSTNGISGALSPIGAYALNGYS
                     ATTGIGVSGANAGTGIGVWGSSSSGYGTVGFSGGNTAFAGMFQNSNANGTGLLVTGNN
                     LAGTYLVNGSAGSFRGTNFGIINFSNISSAAVAGSAVWGSNRKTVITSILGGAGVTGI
                     DSANGSGVIGVSFYGGFEGVTGIGSSNSGTGVLGQAGMGPSPRAVWGDIATVSGTNAT
                     SIAVVGSNNSVGANAVGVYGQEVAAPNGPARYAILANGDMAATGTKPFVIDHPLDPSG
                     KFLKHFSMESNEVLNYYRGNVILDANGEATIQLPSYFNSINNTNYNYNLTPIGAPAQL
                     YVKSEVSGNNFVISGGIAGMKVSWTLTAERNDPYLQQNPDSRLVELVKTGDAAGKYLV
                     PSLYGQPESAGIYYKPSPQKTAVNEKSKDAPLIKSRDFTQKDVSK"
     gene            6134..>8984
                     /locus_tag="PKY09_11050"
     CDS             6134..>8984
                     /locus_tag="PKY09_11050"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB13434.1"
                     /translation="MKKFVYFIFLLLLPVLAWSQCMITPVTLQDRINNATVVVEATAI
                     QQNSYWNNSNDFIYTSTTLSISAVYKGAVQGSQIALITEGGIVGNIMIKSEPSLNIKP
                     GQNGIFFLIPHNQPSGSKLSGMFEGYALSQSFILFDETSNTATGPFENYSDINALRNQ
                     IINATGGVQRISSIAPQPMHPSVNKIMIPPTIASIVPATTTAGTFSIITINGNNFGAT
                     YVNGTSNVEFPDANTGGGSYISAPQNHIQSWNNTQIQVWVPSQGGSGLIRVTNNLGET
                     TTSGISLTVNYNETNVVSGGIYYQPDLINNNGTGGYTFRYNTTFNGNAAAVAAFERAL
                     QTWRCGTFVNFNRSGTSATTCQALDGTNLVTFDGSCALPAGVLGISYSYYSACATGVW
                     YLNENDLKFRTNGTGGINWNYGPAATGGGNFDFESVCLHELGHSHQLGHTILPVTVMN
                     YAIGPNTDRRTLTPISETAGGNDIMSRSVVTNACGPTAMVALTAGNCAINAPIADFNG
                     TPLTGCNSLNVTFTDASLNTPTSWSWSFPGGVPNTFSGQNPPVIFYAAPGNYAVTLTV
                     SNAFGSNTLTKTNYVQIATCPPPTVDFAGNPTTVCEGSTVQFADLSTNSPTSWSWTFP
                     GGIPATSTAQNPVITYATAGVYNVTLTATNAYGSNTLTKTNYITVNNCPAPPVADFNA
                     SPTTLCSGSTVNFTDLSTNTPTSWSWTFAGGTPLSSVAQNPIVTYNTPGTYTVTLTAT
                     NSGGSNTKTVTAYITVNLCSAPVASFSGFPTTICEGQAVTYVDQSTNSPSSWAWTFTG
                     GAPAASALQNPVVSYAAAGTYTVSLTATNAFGNNTFTRTNYINVVTCPSPGTGLIVND
                     GSLIFVQPTAMIHVEGGVINQDNLSNIGTWDNSGQVRIQGDWTNNSSGNAFINSSPGE
                     VELFGANQTIRGTTPTFFFTLNLTGTGIKTQNIDAT"
BASE COUNT         2818 a         1743 c         1732 g         2691 t
ORIGIN      
        1 tgttcctgtt tttatccgcc ttgccaacga tcagagtatg ctggaaatgg cattggttga
       61 aaatattcag cgcgaaaatc taaatgccat tgaaattgct atcagctaca aaagactgat
      121 tgatgaatgt aagcttacac aggaagacct tggacttcgc gtaggcaaag accgcaccac
      181 agtaaccaat tatcttcgtt tgcttaagtt gccaccacaa attcaaactg cacttcgtga
      241 tgataaaata agtatgggac atgcacgtgc cattatcagc attggcgatc ctgttatgca
      301 gttgaaagtt ttcaatgaaa ttcttgaaaa tgatttaagc gttcgttctg ttgaagatat
      361 agtacgtggc gtatcaaaaa atgagaagaa gcaaccaagc atcagcgaaa agtttattaa
      421 agggatagaa tacaaacaca ttcagaaacg tttagaagat aagtttgaaa caaagattga
      481 agtgaaacat cgtaaaaatg gttcagggca aatcatgttt acttattttt ctactgacga
      541 tttaaatcgt ttactcgata ttctggacgc ataaattttt gactgcatga ggttgaggtt
      601 attggttgtt ctgtttttta cagtttactg cgttaatgcc caacaggaca ccatgatttt
      661 taagacaaga acaaatattg ttactcaggc cgacaccctc aaacccgctg ctgatttatc
      721 tcaaaaggtc aataaacatt ctcctcataa agctacaatc tattcagcta ttttacccgg
      781 attaggtcag gcttacaata aaaagtactg gaagattcct gtaatttacg gacttaccgg
      841 agtacttaca tattttgctg ttgacaacaa taaagaatat ataatttata aagatgctta
      901 taaatggcga cttgatgata acagtgctac tgtagataag tttgaaggta tttattcgga
      961 tgaggacttg cggattttaa agaactatta tcgcagaaac cgcgaccttt cttttatcgg
     1021 gatgggcgtt gtttatttgc ttaatgtggt tgatgcagcg gtagatgcac acttgtttta
     1081 ttttgatgtc agtgatgact tgtcattaaa agtgcaaccc ggaatacaac caatgaggca
     1141 tagtgcattt gctggtgtaa aactgagttt acaaataagg taagatatga aaattgcatt
     1201 gttgggatat ggtaaaatgg gtaaggaaat tgaaattgcc ggtaatgaac gtggacatca
     1261 aattgtttta aaaatcaact ctgaaaattt taatcaactt aataaagggt ccttacaaac
     1321 tgctgatgtt gctatagaat tttcacgccc tgaaattgct gttgacaatg ctaaattatg
     1381 tattgatgca cacattccgg taattatcgg cacaaccggt tggtatgagc ataaaacagt
     1441 attagaacaa tattgtattc aaaataatgg gactgctatt tacgcttcta atttcagtac
     1501 gggtgttaat gtatttttta tcatcaatga aatcctttcc cggttaatgt ctcctatgaa
     1561 atatgatgca tggattgaag aagtacatca cattcataaa aaagatattc caagcggcac
     1621 agccataaca ctggctaatg atattctgaa gtataacaaa caatattcag gttgggcaat
     1681 taatgatgaa gcaggaaaac ttcccattta cgcacagcgc attgacgaag taacaggttt
     1741 tcattctgtc tccttccgaa atgaagttga cactattgag attacacaca atgctttcag
     1801 cagaaaaggg tttgcacttg gtgctataaa agctgcagaa atggctttaa caaaaaaggg
     1861 attttatgag tataaaaatc tcttaataga acaaccctga ttttatgctg ataaaaaagc
     1921 gatatgaaac agtaatcagc tacttcagga aaaacatgcc tgtggcagaa accgaattgg
     1981 tttacaaaaa tccatatcaa ttattagttg cagttatcct ctcggcacaa tgtactgaca
     2041 agagagtaaa tatggtcacc cctgaattat tcaaagcttt tccgacagct aaaaaacttt
     2101 cagaaacaga accagaagaa gtttttgagt atattaagtc tataagctat ccaaacaaca
     2161 aggcaaaaca tcttgtcggt atggcgcgta tgcttttgga aaaatttgaa ggtactgttc
     2221 catcagaact tgataatctt attcagttgc ccggtgttgg aagaaaaact gccaatgtaa
     2281 tttcatcagt tgtgttcaat aaagcagcaa tggctgttga tacacacgta tttcgtgtgg
     2341 cagcccgatt aggtcttaca cgcaatgcaa aaaccccttt agctgcagaa aagcaattgg
     2401 taaaacatat tccggaagaa ttaattgcaa ctgcacatca ctggctcatt cttcatggcc
     2461 gttatgtttg cacagctcga aatcctaaat gtaatatttg tggattaaaa gaggtttgtg
     2521 tctattataa aaaacatcct atttaggata atacaccttt accttcgcgc agaacagcaa
     2581 tttcatctgc tgtacaatct acgattgtac ttggcacatt tcctcctgct ccacagtcaa
     2641 caatcatatc aaccatctca ctgaaccttt cataaatttc atcagggtct gtcaaataat
     2701 ctgtaatagg atcattgata tcatgcagag atgccgacat cagtggatgc cccaactgtt
     2761 gaataattgc taatgttact ttctgatcgg gaatacgaat accgatattc ttttttcttg
     2821 ctctgaatat accgggcact tttgttgatg cttcaagaat gaaggtaaac ggtccgggta
     2881 gcgaacgatt caataactta tatacactac ggtcaaatgg tttggtatat tctgcaatgt
     2941 tttttaaatc attacataga aaagaaagat ttgctttttc aggttttaca cctttaaacc
     3001 gacttacttt ctcaatggcc ggcagactgt aaatgctgca tgccaaagca taaactgtat
     3061 cggttggtat gatgacgata ccatcattgt tgatgcattc aacaactttc ttgatttttc
     3121 tgtcatcaat attattctcg tttatctcta tcaacattgc acttttgttt tactgacaac
     3181 aaaaaaacaa caataaggtt aatctttcca atcggcaata aacaaattgg tgtcatgtgt
     3241 gccaccattg tttctgttgc ttgaaaaaat caatttctta ccatcaggag aaaacatagg
     3301 aaatgcatta aacacactct gatcagtaat ttgcactaag ccagttccat cagtattaat
     3361 catatacaac tgaaaattaa atcctgattt tgattcatga tttgatgaga aaagaatttt
     3421 ctttcctgat ggatgaaaga aaggtgccca gtttgcttta ccaagatgcg ttatctgttt
     3481 cagattgctg ccatcaacat tacaagtata aatttccata tttgtgggtg ccaccaaacc
     3541 ctcgcttaac aattgtttgt actcttgaat ctcttccggt gtttttggcc ttgatgaacg
     3601 gaacaccaat tgtttactat cgggcgagaa aaatgcgcct ccgtcatagc ccaatccaaa
     3661 tgttatttgt ttctgattgc tgccatcaat atccattgtc cacaattcaa gatcaccggt
     3721 gcggtcactt gtaaaaacaa tctttttacc atcaggcgaa actgttgctt ccgcatcgta
     3781 gcctggtgca ttggttagtt gtttaactac ttttccattc aggtctgcaa caaaaatatc
     3841 gtaggtatta aaaatcggcc acaggtattt tccaccccgc ctaaggtcac ctgtttcagg
     3901 acatttatct cctcctaaat gtgtgcttgc ataaagaatt gttttattac cgggcataaa
     3961 atacgagcag gtggtacgac ccttaccggt actgattaaa ggaggtttgc tatctgattt
     4021 ccagttgttc aggtctaata aaaatatctg atcacattca acaccccatg ctttgttgtt
     4081 tgattgaaaa gccaatttat tgttgtcaaa actaaaataa gcctcggcat tatcgccacc
     4141 gaaagttaat tgtttaatat tcgtaagatg cttattttca atagtatcaa catcttgagc
     4201 taaggataag tttaagcaaa aaggcaacag aattaatagg cacttaaatc gtttcataat
     4261 tttaaaatat ttggtcgcga aagtatccct tttaagagtg caacttggcg tgatttttgt
     4321 cattaattgt gaaaatgaca aaatcattac aagaccctct taccgatatt tttctacaaa
     4381 aacatttgtt ttgtgatttc cttaacatat ctttgaagtt actctaaata gaataaagat
     4441 gaaaagaatt ttatctgttt tactcattat tctgattgct catgcaagtc aggcacaaaa
     4501 tgtaggaatt ggcactaatg ttccaatgca aaaattagat gttattggaa aaataagatc
     4561 atcaacactc actggtgttg gtaatcgttt tgttatggca gacccaacag gtacgctcaa
     4621 tgtaagttca agtggtctgc ttgattctac tgcatggcta acactgggaa atagttcaat
     4681 agatacaaca aagaattttc ttggaacatt aaccaacacc catattttat ttaaaacaaa
     4741 tggcaatgct gctgcaaacg aaagaatgcg catccgttca ggtggggaaa ttgtggtaaa
     4801 caacacttct gtcggcaaca acaacggtga tgtgttttct gcatatgcca caggaagtac
     4861 taatggtata agtggagcac taagtccaat tggtgcatac gcattaaacg gctattcagc
     4921 aacaacaggc attggtgttt caggagctaa cgcaggcaca ggcataggtg tttggggcag
     4981 ttcatcttcc ggttatggaa ctgtaggatt ttcaggtggc aatacagctt ttgcaggaat
     5041 gtttcaaaat tcaaatgcta atggaaccgg attgcttgtt acaggaaaca atcttgcagg
     5101 cacttattta gtcaatggtt ctgcaggctc tttcagagga actaatttcg gaataattaa
     5161 tttctcaaac atcagctcgg cagccgttgc aggtagtgca gtttggggta gtaatagaaa
     5221 aacagtcata acaagtattc tcggtggtgc cggtgtcaca ggaattgatt ctgccaatgg
     5281 ctcaggtgtt attggtgtat cattttatgg aggatttgaa ggtgttaccg gaataggtag
     5341 ttcaaatagt ggaaccggtg ttttaggaca agcaggaatg ggcccctcac ctcgtgctgt
     5401 atggggtgat atagcaacag tttcaggcac gaatgctacc tctattgctg ttgtaggaag
     5461 taataattca gttggtgcca atgcagtagg tgtatatggt caggaagtgg ctgcacccaa
     5521 tggtcctgca cgttatgcca ttttagctaa tggtgatatg gcagctacag gcacaaaacc
     5581 ttttgtaatt gatcatccac tcgatccatc aggaaaattt ctaaaacatt tttctatgga
     5641 atcaaatgaa gtgcttaatt attacagagg taatgttatt ttagatgcaa atggagaggc
     5701 aactattcaa cttccatcat atttcaatag tataaacaac acaaactaca actataacct
     5761 tacacctatt ggtgcacctg cacagcttta tgttaaatca gaagtttcgg gtaataactt
     5821 cgtaatatcc gggggcattg ccggaatgaa agtaagttgg actcttactg ctgaaagaaa
     5881 tgatccctat ttacaacaaa atccagatag tcgtttggtt gaattagtta aaaccggtga
     5941 tgctgcaggg aaatatttgg ttccatcatt atatgggcaa cccgagtcag caggaattta
     6001 ctacaaacct agtcctcaaa aaactgctgt aaacgaaaag tcgaaagatg caccgttaat
     6061 taaatcaaga gattttacgc aaaaggacgt ttctaaataa caagctcatt tcatcattaa
     6121 agacaacatg agtatgaaaa agtttgttta ctttatattt ttattgctac ttcctgtttt
     6181 ggcatggtcg cagtgtatga taacacctgt taccttacag gatagaatca acaatgccac
     6241 tgttgtagtt gaggccacag caatacaaca aaacagttac tggaacaaca gcaacgattt
     6301 tatctacact tccactacac tttctatttc tgcagtatat aaaggcgcag ttcagggaag
     6361 tcagattgca ttgataacag aaggtggtat tgttggaaat atcatgataa agtcagaacc
     6421 atctttgaat attaaacccg gacagaacgg aatatttttt ctgatacctc acaaccagcc
     6481 ttcaggaagt aaactgtcag gaatgtttga gggatatgct ttgagtcaga gttttatttt
     6541 atttgatgaa acaagtaata cagcaacagg tccgtttgaa aattacagtg acatcaatgc
     6601 attacgaaat cagattatca atgctacagg tggcgttcag cgtatttcat ctattgctcc
     6661 tcagccaatg catccctcag taaataaaat aatgattccg ccaacaattg cttctattgt
     6721 accggccaca actactgcag gaacattttc tatcatcaca attaacggaa ataattttgg
     6781 tgctacctat gttaatggaa cgtcaaacgt tgaatttcct gatgccaata caggaggtgg
     6841 aagttatatt tcggctccac aaaatcatat tcaatcgtgg aacaacacac aaattcaggt
     6901 ttgggtaccc tcacaaggtg gcagtggttt gattagagtt accaataatc tgggtgaaac
     6961 tacaacatcc ggaatttcct taaccgttaa ctataatgag accaacgtgg tgagtggtgg
     7021 tatttattat caacctgact tgataaacaa taatggaacc ggaggttata cctttcgtta
     7081 caacacaacc tttaatggta atgctgctgc tgttgctgca tttgaaagag cattacaaac
     7141 atggcgctgt ggtacttttg taaatttcaa ccgttcgggc acttctgcaa caacttgtca
     7201 ggcattagat ggaaccaact tagtgacctt tgatggttca tgtgcattgc ctgcaggtgt
     7261 actcggaatt tcatatagtt attacagtgc ctgtgccact ggtgtttggt atcttaacga
     7321 gaatgattta aagtttcgta ccaatggaac cggaggcata aactggaact atggtcctgc
     7381 tgcaaccggt ggtggcaact ttgattttga atcagtttgt ttgcatgaac tcggtcacag
     7441 ccatcagtta gggcacacaa ttttacctgt aacggtgatg aattatgcca ttggcccaaa
     7501 tactgacaga cgtacactaa cacccataag tgaaactgca ggtggtaatg atataatgag
     7561 tcgtagtgtg gtgactaatg cttgtggacc aacagctatg gttgcactca ctgccggcaa
     7621 ctgtgcaatc aatgcaccta ttgcagattt taacggcact ccactgacag gatgtaactc
     7681 acttaacgta acatttacag atgcttcttt aaatacacca acatcgtggt cgtggtcatt
     7741 tccgggagga gtacccaata cattttcagg acaaaatcct cctgtaattt tttatgccgc
     7801 acccggcaat tacgcagtta ccttgactgt cagcaatgca tttggcagca acacgcttac
     7861 taaaacaaat tatgttcaga ttgcaacctg cccgccacca acagttgact ttgccggaaa
     7921 cccaacaaca gtgtgcgaag gtagcacagt acagtttgca gatttaagta ccaattcacc
     7981 aacaagctgg agttggacat tccccggagg aatacctgca acatctactg cacaaaatcc
     8041 tgtgattacc tatgcaaccg ctggtgttta taatgttact ttaactgcaa caaatgcata
     8101 tggcagcaac acacttacta aaacaaatta cattacagta aacaattgtc ctgctccacc
     8161 ggtggctgac tttaatgcct ctccaacaac tttgtgttcc ggtagcactg taaacttcac
     8221 agacctttca accaacacac ccacatcgtg gtcgtggaca tttgccggtg gcacaccgct
     8281 ttcatctgta gcacaaaacc caattgttac ctacaataca ccaggaactt atacggttac
     8341 tttaacagca acaaacagcg gtggctctaa caccaaaaca gttactgcat atattacagt
     8401 aaatctttgc tctgcccctg ttgcttcttt ttcaggattt ccgaccacca tctgcgaggg
     8461 tcaggcagta acttatgttg atcagtcaac caactcacca tcatcatggg catggacttt
     8521 taccggtggt gcacctgctg catctgcatt gcaaaatcct gttgtcagct atgctgctgc
     8581 cggtacctac acagtttcat taactgcaac aaatgctttt ggtaacaata cttttacaag
     8641 aacaaattac atcaatgttg ttacatgtcc atcaccggga acgggactta tagtcaatga
     8701 cggatcattg atttttgttc aacctacggc aatgatacat gttgaaggtg gtgttattaa
     8761 tcaggataac ctgagtaata ttggcacatg ggataactcc ggtcaggtgc gtattcaagg
     8821 cgactggaca aacaattcat caggaaatgc cttcatcaac agcagcccgg gtgaagtaga
     8881 actgttcggt gccaaccaaa ccataagagg tacaacacca acatttttct ttacattaaa
     8941 tctaacaggt acagggatta aaacccaaaa catagatgca acgg
//
LOCUS       DAOQNP010000094         8897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_2909_length_8897_cov_10.243045, whole genome shotgun sequence.
ACCESSION   DAOQNP010000094 DAOQNP010000000
VERSION     DAOQNP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2909_length_8897_cov_10.243045"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..1139)
                     /locus_tag="PKY09_11055"
     CDS             complement(369..1139)
                     /locus_tag="PKY09_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009279736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNB13435.1"
                     /translation="MKAIIIEDESLIARELKNKIASVAPDVIITETLASVKTASRWLM
                     ENAEPDLVFADIQLSDGVSFEIFERYDLKCPIIFTTAYDEYALRAFKVNGIDYLLKPV
                     DVNELSKAIDKCRTIIENKTKLPGNLQELMQLLQNPSSNTKLYKEKFVIKVRNNWLPI
                     MTKDIACFIRDNLNYIYTFSGEKYITDYVTLEEIEELLDPALFFRTNRQSIVHIDAIQ
                     SIKPGDTKKLFITLKSPLKIELDISREKAPGFKKWFER"
     gene            complement(1150..4188)
                     /locus_tag="PKY09_11060"
     CDS             complement(1150..4188)
                     /locus_tag="PKY09_11060"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB13436.1"
                     /translation="MRILKMQIKKWLLPALLLFSGMAFSQSAAINFHKLGFAEGLHDG
                     IIKCIAQDRFGYTWIGTVGAINRFDGKKITQFTNIPGDTSTPYSSQPRCMHSDMAGRF
                     WIASETGLTEYNFTTNKFIRVPLLKDIIIFSIESINDSILLIGARGGYFRYNTKTGMA
                     FNYAASQNPMHTAVRPKTVMNIFKKNNLLYLATDKGLLIHDVLKDEIKEVELPELKNL
                     PILSVCNDRDGTTWLCAKGAVKFAKISPNLKQFTILDNYFTGGLNVKIPEVVSLLLDK
                     KERLWLITVDDGLMQFHPSTNTFTRYLHNVHYASGPSSNSYRHIYGDKSGIIWLGFNG
                     EGVNYFEPDQNLFSTIFPFPLEMSDRLKKMGRGIAVDKTGNIWMGNHDGLSRYNPVNN
                     QYTVWRNEEGRSKVLYNNSIRTLLCDKDNNIWIGTGSGVNRYNAATGKIEFIDSRKLP
                     LSYYISINADRSGNIWFCTTDTVALYWYSLADKTYHNISQHPQLKKYKTFSATSYLME
                     DSKQRLWISFSRKGMLMLDKKTGTTKLYAASDSTTKGLIGNQVIDIKEDNNGWVWATT
                     LNGITGIDADNNRFLSFNNKNGLPGNMAGPLAIDSSNRIWIGVNGGLTMLSADRKKLS
                     VFSENEGLPSVGFNEHAGITAPNGDIIMPTNNGFFRFNPGTYKERKLAFNFYVSGYTV
                     FDKDFYRINVADENPSILLKPSQSSFTFYLDALNYSNPTQTWFAYKLEGFEKDWHYTQ
                     DPKAVYTNVPGGHYQFLYKATVNNSNWDAVVAKKISVHLSTWFYKTIWFKTLFGLLIA
                     ALLFAIYRYRTRQQNQVYQLKEKAQALEKEKAQINYETLKQQLNPHFLFNSLTSLNSL
                     IDADPKAASEFLGSLSKTYRYILKNRDTETVSLADEIRFAENYVKLQQTRFEKGFDVH
                     INIPEEYHHRKIVPVTLQNLIENAIKHNIIDEESPLVVNIFVEEDMLVVKNNLQKKKF
                     VETSNRQGLANMQSLYRYLSNRPVEITETNNSFTIHIPLL"
     gene            complement(4380..5141)
                     /locus_tag="PKY09_11065"
     CDS             complement(4380..5141)
                     /locus_tag="PKY09_11065"
                     /inference="COORDINATES: protein motif:HMM:NF026626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4595 domain-containing protein"
                     /protein_id="HNB13437.1"
                     /translation="MRKVFIALTAIIALSSCEKEDVSIPAAISKTLSKTSYVYNGNTP
                     ETEEFTFDTNGKIVSIKENDRTHTFNFVSASSLEVTERSNSNSSIINIRHCVLNDKGF
                     VTQMVVKNASGTISGTYDYTYNAEGFQTGRQLTYPNGDIYKNEYSYADGNLISSKTYK
                     NNVLESHTNITYDNTKANKTRLNFWTSYWDVPGLFGKETKYLLSETKGYNNSNTLISH
                     DQYTFELDADGYPLKETRNFVLTGKLGVKNYTFIK"
     gene            complement(5351..5677)
                     /locus_tag="PKY09_11070"
     CDS             complement(5351..5677)
                     /locus_tag="PKY09_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13438.1"
                     /translation="MKQILSFNWLKKMFTSDDKAKKLQLLREQRQILCNGVQTTAEVI
                     DTGMQNDIVGNMVQIRLMLKLKKADGSYLYSNTASLVTLRHLPGKGDLLHIKYIPDSL
                     DMVVIL"
     gene            6057..7310
                     /locus_tag="PKY09_11075"
     CDS             6057..7310
                     /locus_tag="PKY09_11075"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNB13439.1"
                     /translation="MYAQNVGINGDGSAPNSSAGLDIKFSDKGLLIPRVAINDVNLAA
                     PIATPAEGLLVYNETGTQAKGFYYWNGTKWVLLLTGSGANNAWKLTGNAGTVDSAHFI
                     GTTDNVSLNFKANNGKAGRIGLSGDASVFFGYQAGNADDHIDNQNVGIGYQALFSNTT
                     GYNNVANGYQSLYSNTIGFSNQANGYQSLYSNTTGYQNVANGYRSLYSNTYGYYNTAV
                     GLSSLNSNTGGYENVANGFYALYSNTTGFNNTGVGAYALNLVTTGSNNTSLGYNAQVA
                     LPTESNQVRIGNTYVTYAEVQVAWAIGSDRRWKTNIQESPLGLSFIQSLRPVVYTRKN
                     DEKQRHEYGFIAQEVEEALQKAGVNPQGVITINDEGMYSVRYNDFIAPMVKSIQELST
                     ENAALKKKNEELENRLERLEKLMVK"
     gene            7404..>8897
                     /locus_tag="PKY09_11080"
     CDS             7404..>8897
                     /locus_tag="PKY09_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13440.1"
                     /translation="MREIFFICLLITSRLFAQGVINNGANIVVSNNANIVIDGAAGHY
                     TNQSGGLLSTPTANGMVKLQGNWVNNASNVAFSNDGITTELNGASQNIGGTNSTTFYN
                     LTLSGTGTKTLNIATKAGGIVTTSGVVALNDRLLDLNQNELEITNTSTGAFTRTSGYV
                     ISETNAATNPSIIKWNTAGAGNYIFPFGASGDYIPLGLNITTAGASTISASTRATPTS
                     NNKLWTTGVTNMASAVLGLPDASIVSVIDRWYEVQTSTAITADLTLSYRGTENTTTLA
                     PTGTFALQRWNGTWEPQQGTGAGVTSGVGSVTASNVSEFSTFVLTNTSGPLPVALKSF
                     QAYCGLRSINIEWETASESNLSYFVIERSVDAIQWDFVSQITAKGSTTSTAKYKVTDD
                     LQHKTIYYRLKMIGLEGKEEISKIVSTSCRGSNNGLNVYPNPTTGVFSVSLRSEDNLG
                     LATVEILDITGRMVHSQQVEIISGETLVQFVTDRATTRHLSVYNPGAD"
BASE COUNT         2661 a         1700 c         1723 g         2813 t
ORIGIN      
        1 aaatatattt ctttagattc attttggtct atttaaatat tcattttcgt attagtgtaa
       61 atctcagttg gatattccgg agtaaaggaa gccattgatt tcggagctaa gtgagccacc
      121 ccgggcggga ggatttcgga acaatgtgcg ttccatgtac tatggccatt acaaatgttg
      181 aattttgcaa ttcagcgcta ttgcaaaact gatgtgcccg tttcacaact atgttataca
      241 catcttgtca attgtccaat atagagttgg ttttttgatg ttgagcaagt atgcaaggca
      301 aaaaacaatg aacaccacaa ttattcttta aggttcagtt agaacaattt gttccagcgt
      361 aaataccttt accgctcaaa ccatttttta aatcccgggg ctttttcccg gcttatatcc
      421 agctcgatct tcagaggtga ttttagcgta ataaaaagtt ttttggtgtc gccgggtttt
      481 atactttgta tggcatcaat atgaacaata ctttgcctgt ttgttctgaa aaacaaggct
      541 gggtcaagga gctcttctat ttcctctaac gtaacatagt ccgttatgta tttttctccg
      601 ctgaaggtat agatgtaatt gaggttgtct cttataaagc aggctatatc ctttgtcata
      661 atcggaagcc agttgtttcg taccttgata acaaattttt ctttatacag cttagtgttt
      721 gaagaagggt tttgcaatag ctgcatcagc tcctgcagat tgcccggaag ctttgttttg
      781 ttttcaataa tcgttctgca tttatcaatt gctttgctca actcatttac atcaaccggc
      841 tttagtaaat aatcaatacc gtttacttta aatgccctta atgcatactc atcataagcg
      901 gtagtaaata tgatagggca ctttaaatca tagcgttcaa aaatttcaaa acttacccca
      961 tcgctcagct gaatatctgc aaacaccaaa tccggttcgg cattttccat cagccaacgg
     1021 ctagctgttt ttacactggc cagcgtttcg gtaataataa catccggtgc aacgcttgca
     1081 attttattct ttagctccct ggctatcaga ctttcgtctt ctataataat tgctttcata
     1141 tttatctgtt tataataagg gaatatgtat ggtaaaagag ttattcgttt ctgtaatttc
     1201 cacaggcctg ttgcttaaat accggtacag cgactgcata tttgccaaac cctgcctgtt
     1261 gctggtttca acaaatttct ttttttgtaa attgtttttt acaaccagca tatcttcttc
     1321 tacaaaaata tttacaacca atgggctttc ttcatcaata atattgtgtt taattgcatt
     1381 ttcaataagg ttctgcagag ttactggcac aattttacgg tgatggtatt cttccggaat
     1441 attgatgtgt acatcaaatc ctttttcaaa acgggtttgt tgaagcttta catagttttc
     1501 tgcaaaacgt atttcatcgg ccagtgaaac tgtttcggta tcccggtttt ttaaaatata
     1561 cctgtaggtt ttgctcaggc tgcccaaaaa ttcactggct gctttggggt cagcatctat
     1621 caggctattg agcgaagtaa gtgagttaaa cagaaaatgt gggtttaatt gttgcttcag
     1681 agtttcataa tttatctgtg ctttttcttt ttcaagggct tgtgcttttt ctttaagttg
     1741 atacacctga ttttgctggc gggtgcgata gcggtatatg gcaaataata aagcagcaat
     1801 caacagccca aacaacgttt taaaccaaat tgttttataa aaccatgtac taagatgtac
     1861 gctgattttt ttggccacta ctgcatccca attactgttg ttcactgtgg ctttgtataa
     1921 gaattggtaa tggccacccg gtacattggt ataaacagcc ttggggtcct gtgtatagtg
     1981 ccagtctttt tcaaagcctt ctaacttata agcaaaccat gtttgtgtag gattgctgta
     2041 gttgagtgca tccaggtaaa aagtaaaaga gctttgtgag ggctttagca aaatagatgg
     2101 attttcatct gctacattta ttctgtaaaa atctttatca aaaacggtat agcctgaaac
     2161 ataaaagttg aaagcaagtt ttcgctcttt gtatgtacca ggattaaagc ggaaaaagcc
     2221 gttgttggta ggcataataa tgtcgccgtt cggtgcagta ataccggcat gctcattaaa
     2281 acctacagaa ggcaaccctt cgttttccga gaaaacagat agctttttac ggtcggcgct
     2341 cagcatagta agcccgccat ttacacctat ccaaattcgg ttactgctat caatggcaag
     2401 cgggccggcc atattgccgg gcaagccgtt tttgttatta aaggaaagaa accggttatt
     2461 atctgcatct atacccgtta taccatttaa tgtggtggcc caaacccagc cgttattatc
     2521 ttccttaata tctataacct gattgccaat aagccctttt gttgtgctgt ctgatgcggc
     2581 atataatttt gttgtgccgg tttttttatc cagcatcagc attcccttgc gggagaatga
     2641 aatccataag cgttgcttac tatcttccat aagataggag gtagcggaaa atgttttata
     2701 ttttttcagc tgcggatgct ggctgatgtt gtgatacgtt ttatcggcaa gggaatacca
     2761 atataatgct actgtatcgg tggtacaaaa ccatatattg ccgctacggt cggcattaat
     2821 agaaatataa taacttaacg gcaacttgcg gctgtcaata aattcaattt ttcctgttgc
     2881 ggcattatac ctgtttacac cgctaccggt acctatccat atattattgt ccttatcgca
     2941 aagtagcgta cgaatactat tattgtaaag caccttgcta cggccttctt catttctcca
     3001 aacggtatat tgattattga ccgggttgta gcggcttaag ccatcatgat tacccatcca
     3061 gatattaccg gttttatcta ctgcaatacc ccgtcccatt ttttttagcc ggtcgctcat
     3121 ttctaagggg aatgggaaaa tggtggaaaa caggttttgg tcgggttcaa aataatttac
     3181 accttccccg ttaaagccaa gccagatgat gcccgactta tcgccgtaaa tatgccgata
     3241 actgttagaa gaagggcctg atgcataatg aacattatgc aggtagcggg taaacgtatt
     3301 ggtggatggg tgaaactgca ttaaaccatc atcaacagtt atcagccata atctttcttt
     3361 tttatcaagc agtaaactta ctacttcagg aattttcaca tttaaaccgc cggtaaaata
     3421 attatccagt atggtaaact gtttgaggtt gggagaaatt tttgcaaact ttaccgcccc
     3481 ttttgcacaa agccaggtag tgccatcacg atcgttgcat acagataaaa tgggcaggtt
     3541 ttttaattcc ggaagctcaa cctccttgat ctcatccttt aacacatcat gaataagcag
     3601 gcctttgtcg gtggccaggt acaataagtt gttcttttta aaaatattca ttaccgtttt
     3661 aggcctaaca gctgtatgca ttgggttttg agatgcagca taattaaatg ccattccggt
     3721 tttggtatta tacctgaaat aaccaccacg ggcaccaatc aacaaaatgc tgtcattgat
     3781 gctttctatg gaaaaaataa taatatcctt taacagaggc acccgtataa atttgtttgt
     3841 ggtgaagttg tattcggtta agcccgtttc gctggctatc caaaagcggc ccgccatatc
     3901 cgaatgcata cagcgtggtt gcgaactgta aggagttgac gtatcaccgg gtatattggt
     3961 aaactgtgtt attttttttc catcaaaccg gttgatggcg cctactgttc ctatccatgt
     4021 gtacccaaac cggtcttgtg caatacattt tataattcca tcgtgcagac cttcggcaaa
     4081 acccagttta tgaaaattaa tggcagcaga ttgcgaaaaa gccatgcctg aaaaaagtaa
     4141 caaggcaggc agcagccatt tttttatctg catcttcaat attctcattt tctttaaaca
     4201 ttgcaagata caaactattg ttcaatacaa aatagcccta ataatgaagc ggggcatttg
     4261 taaaataaag tgtatcaaaa atttaatgag tcgtttgatt tacacttaaa tttatttgct
     4321 attgggatta aaatgtgtgg tcatttcagg ataaaataca gcaacgttta atgaatgggt
     4381 tactttatga aagtgtaatt ttttactccc aacttgcccg ttaatacaaa attgcgtgtt
     4441 tcctttaaag gatagccatc agcatcaagt tcaaatgtat attggtcgtg ggaaataagg
     4501 gtgttggaat tgttgtaccc ttttgtctca ctcaacaggt actttgtttc tttaccaaat
     4561 agacctggca catcccaata tgatgtccaa aaattcaacc gggttttatt ggccttagta
     4621 ttatcgtagg taatattagt gtgagattct aaaacattat ttttatacgt cttactggaa
     4681 atcaggtttc catctgcata actgtactca tttttatata tatcgccatt ggggtatgta
     4741 agttgtctgc cggtttgaaa accttctgca ttgtacgtgt aatcgtaggt tcctgagatt
     4801 gtaccactgg catttttaac caccatttgc gtcacaaaac ctttgtcgtt caaaacacag
     4861 tgcctgatgt taattatgga actgttggaa ttggatctct cggttacttc taaagatgaa
     4921 gcagacacaa aattaaacgt gtgggtacgg tcattctctt ttatagagac aattttgcca
     4981 ttggtgtcaa atgtaaactc ttcggtttca ggtgtattac cgttataaac ataagacgtt
     5041 tttgaaagtg ttttactgat cgcagcagga atagatacat cttctttctc acaacttgac
     5101 aatgcaataa tagctgttaa tgcaataaat acttttctca ttttctattt tttatttgat
     5161 tgatgctgta aaattgcagc catactacct gtaaaaaaat cttaaagcaa ctacctgtag
     5221 ctacttttaa ataaagtgta ccaaatcttg attaaagtgt taggggttgt attactaatg
     5281 atcgggttta taataaaata aaaccacagt ttagaggctg tgaaggataa ggatcatgat
     5341 gaatatatta ttacagaatt actaccatgt ctaagctatc gggtatatat ttaatatgca
     5401 gcaaatctcc tttaccgggt aagtgcctca gcgttaccag gctggcggtg ttggaatata
     5461 aataggagcc atcggctttt ttaagtttta gcataagcct tatctgtacc atatttccta
     5521 ctatatcatt ttgcatgccg gtgtctatta cttcagcagt agtctgaact ccgttgcata
     5581 atatctgccg ctgctccctt agcagttgca gttttttagc tttgtcatct gacgtaaaca
     5641 ttttttttag ccagttaaat gaaagtatct gtttcatcgt gcaaacatca tattatatac
     5701 atttcataaa aaataatacc acacttactg tacctctttt gtaataaagc gtatttattt
     5761 acgattaaag tgttttctaa acagcagttt caaatccggg aaaatagatc tgtaggttat
     5821 gttttgtatc aatcgttttt gcttaacctg tttcaagcaa aacacctcgt ttttattttg
     5881 gcgcacttca ttataaacat gcacctgatg gtgcgataaa aataggatgg tagtctttgt
     5941 caaatcaatt ttgtgatgtc aaaataaata tttaatcatt taatacttaa aagaaaatga
     6001 aaagaatttt aactctcata tgcacaattg ctttgtttct atctttcaat gcacaagtgt
     6061 acgcccaaaa tgtaggcatc aatggagatg gatcggcacc caactcgagt gcaggcttag
     6121 acattaaatt ctctgataaa ggcttgctca ttcctcgtgt ggcgatcaat gatgtaaacc
     6181 tcgctgcacc gattgccaca cctgcagagg gcttgttggt ttacaacgaa acaggtacgc
     6241 aagccaaagg attttactat tggaatggca caaaatgggt attgctactt actggctctg
     6301 gtgctaacaa cgcctggaag ctcacaggca atgcaggtac agttgacagt gcacatttca
     6361 ttggaacaac tgataatgta tcgttaaatt ttaaagccaa caatggaaaa gcaggacgaa
     6421 ttggtctatc aggcgatgct agtgttttct ttggctacca ggcaggaaat gccgatgacc
     6481 atatcgacaa tcagaatgtt ggtataggat atcaagcctt attttccaac acaacaggat
     6541 acaataatgt agcaaatggt tatcaatcct tatattccaa cacaataggt tttagtaacc
     6601 aggcaaatgg ttatcaatcc ttatattcca acacaacagg ataccaaaat gtagctaatg
     6661 gttatcgatc cttatattcc aacacatacg ggtattacaa cacagctgta ggtttatctt
     6721 ccttaaattc caacacagga gggtacgaaa atgtagcaaa tggtttttat gcactatatt
     6781 cgaacacaac agggtttaat aacacaggtg tgggtgctta tgccttaaat ctcgtcacaa
     6841 cagggtctaa caacacatct ttaggttata atgcacaagt ggctcttcct acagagagca
     6901 atcaagtacg cataggaaat acatatgtta cctatgcaga agtacaagta gcatgggcaa
     6961 ttggtagtga tcgtcgctgg aaaacaaata ttcaggaaag tccgttgggc ttatccttca
     7021 tccaatcatt gcgtccggtg gtttatacaa gaaaaaatga tgaaaagcaa aggcatgaat
     7081 atggttttat agctcaagaa gtggaagaag cactacaaaa agcaggtgtt aatccacaag
     7141 gggttatcac cataaatgat gaaggaatgt atagtgtgcg ctacaacgac ttcattgccc
     7201 ctatggtcaa atccatccag gaactcagta cagaaaatgc agcactaaaa aagaaaaacg
     7261 aagaattgga aaatcgtttg gagcgtttgg agaaattgat ggttaagtaa gcctaagatt
     7321 ttaaccgcaa aaattgcaga ggagcaaatc ttttcgtcct ttgcggtcaa taataatcat
     7381 caatcaaatt ataagaaaga attatgagag aaatattttt tatttgccta ttaattacat
     7441 caaggttatt tgcccagggt gtaatcaata atggcgcaaa cattgtagtg tctaacaatg
     7501 cgaatattgt tattgacggc gctgcaggtc attacaccaa ccaaagtgga ggtttgctaa
     7561 gcacacccac agcaaacggc atggtaaagt tgcaaggtaa ctgggtgaat aacgcctcca
     7621 atgttgcttt ttccaatgac ggcataacca ctgaactcaa tggagcaagt caaaacatag
     7681 gaggaacgaa ctctaccaca ttttacaacc ttacactgtc cggtacaggt actaaaacat
     7741 taaatattgc gacaaaggca ggaggtattg ttacaacatc aggtgtagta gcccttaacg
     7801 atcgtttatt ggatttgaat caaaatgagt tagaaataac caatacctcc acaggtgcct
     7861 ttacgcgcac aagtggttat gtaatcagtg aaaccaatgc agcaactaat ccgagtatta
     7921 tcaaatggaa tacggccgga gctggtaatt atatttttcc gtttggtgca tctggcgatt
     7981 acattccttt gggtttaaac ataaccactg caggtgctag taccatatcg gcttctaccc
     8041 gagcaacacc cactagcaat aacaagctat ggactacagg agtcaccaat atggcctctg
     8101 ctgttttggg tcttcctgac gctagtatag tatcagttat agatcgctgg tacgaagtgc
     8161 aaacatcaac tgctattaca gcagacttaa cgctgtctta tcgtggtact gaaaacacta
     8221 caacacttgc ccctaccgga acatttgccc tgcaacgttg gaatggaact tgggaacctc
     8281 aacaaggaac aggagcaggg gtgacttcag gtgttggtag tgtgactgca agcaatgtat
     8341 ctgaattttc aacttttgtg ttgacaaaca ccagtggtcc tctacctgtt gcgctaaaat
     8401 cgtttcaagc atactgtggt ttgcgttcta tcaacatcga atgggagaca gcatctgaaa
     8461 gcaatttgtc ttactttgtc atagagcgca gcgttgatgc tattcaatgg gattttgtca
     8521 gtcaaattac tgcaaaagga agcactactt cgacagcaaa gtataaggtt acagacgatt
     8581 tacaacacaa aacaatctac tatcgattaa agatgatagg cttagaaggg aaagaagaga
     8641 ttagcaaaat agtaagtact tcttgtagag gaagtaacaa tggtttgaat gtttatccta
     8701 atcctacaac tggagtattt tccgtttcac ttagatcaga agataattta gggctagcaa
     8761 ctgtcgaaat attagacata accggtagaa tggttcactc tcaacaagtt gaaattattt
     8821 ccggggaaac acttgtacaa ttcgtaaccg accgagcaac tacacgtcat ttatctgtat
     8881 ataacccggg agcagat
//
LOCUS       DAOQNP010000095         8661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3009_length_8661_cov_8.433767, whole genome shotgun sequence.
ACCESSION   DAOQNP010000095 DAOQNP010000000
VERSION     DAOQNP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_8661_cov_8.433767"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..>5927
                     /locus_tag="PKY09_11085"
     CDS             336..>5927
                     /locus_tag="PKY09_11085"
                     /inference="COORDINATES: protein motif:HMM:NF036499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13441.1"
                     /translation="MAQKIYFANAVSEMTTEIRGVINKQFRKFKILSISLLFIHLLFA
                     ALSLLQVANAQTYAPVSYWTFNSSPFNKDSMNRTNMDFTSYGCGYTVSNNSAVGKGLI
                     LGSSGDNINTGNFVTDTSFAIEFLFKPGYQFNQTLLFRRMDGGFSANFNFDGIYFYTY
                     TSGSGSDELKVSFDGIGKRSYGYFVDGNWHHIVFQYSSLTGKKEIYIDGECPSGYSKN
                     ISGKVVNGSNTSLFWNQGVSYMKYFGEYDEIAIYNRTVPAKLIYQHYKDAVQTNKSYS
                     FNLTFNGSLPSPSPISAGLDNTEFAPGHPNPNVSAIDQLRSYPVARYKAGNTLMKNFN
                     WVDLMYMGGRFQSGVSDAQSATNSTAINEELAKNWNYMIQLRLSMDQWGNAWANLANQ
                     NPQLGTSLITLRAQLNPSLIRSQNLSSDKYLQNSSGNFIDPNGNNSSTKWWRPTAPAN
                     AYSGDGNQMKSNVQDVLNALTRPLTMINENGEVFAWSTDAALSADPAVVAAKNASGLD
                     FQTFQARKFKENETQSYRDIIMDIPALSNTKFTEYGIDGFPQYRQKYSEARLVNSEIN
                     GQRYSTPDFYPRWPHNWRNWITAWHGWQWIVDSRYYELQAGDNLFSPFVAAGWDVNED
                     NNMRPAQFMGLLKCLNMIGAEFFYTGYFNEAGNYNPPNPPPANPAGYAWQTAMPSYAQ
                     AVASRYEEFLRNGSVMPGDVPAYYSAPSGKPGYTFWAGDLRKLVVVRKHNTQSKFAIT
                     GTIQPNTSMVGGAENESVATITLDGQQVKFKVRRQGSTYIYDKTNASSPVFYQLDEWH
                     EKSHPSFWSKDFTIESELYDNTNAAYNLKTIQPQGAAAGDYSNFTTAVVFPSTQTTFT
                     PLEYVFQPRSSSQANLYLFVRARSTDGTATGLGVSVDNGASKSIGCVTDTNWTWYRLD
                     ACSQQAIQFTGLSIGSHTIRFAPTNNKIMIDKFILSANNNVITNYSMVACNSGGTATI
                     NASGPTTFCQGGSVTLTAGAGNSYLWSNGATTQSITVSQAGNYVVTVSQGSGCAAISN
                     PVAVSVSSAPAATISANGSTSLCQGASVTLTASAGSTYQWSNGATSQSITVNAAGNYV
                     VTVSNAQGCSATSAATNVTMTAGGTATITAGGPTTFCPGGNVTLTANTGTSYLWSTGA
                     TTKSITVNATGSYSVTVNNGSCSATSAPTSVTVSNSATATITANGPTTFCPGSNVTLT
                     ANAGSSYQWSNGATTQSITVGAAGSYAVTVSNGNCSATSAPTVVTVSNNATATITAGG
                     PTSFCQGGSVTLTANTGSSYLWSNGATTKSITVNASGSYSVTVSTGSCSATSAPKVVT
                     VTQAPNATITAGGPTSFCQGGSVTLTSSTGTSYLWSNGATTKSITVSAAGSYVVTVSN
                     GSCSAVSAPQTVAVAANPTATITPNGSTMLCSGASVVLKASSGTSYLWSTGATTQSIT
                     VSTAGSYTVKVYNAQGCVTTSAPIAITTGSASATIIANGPTTFCQGGGVTLSAGAGTS
                     YQWSNGATTQDINVSTSGSYVVTVSNGSCSATSAPLVITVNPLPAATITASGSTTITQ
                     GQSVTLTASSGSSYLWSTGATTSSITVNTAGIYTVKVTNAQGCSATSSPMVITVVNNS
                     QPASITTTAANNQICMGQSATLTSSPGMQYIWLPGLQATQSIVVNAPGTYTVNIRNSD
                     GSTSTANITISQAPTPNKPEVTYTYVTGTMYQLKAFEPSAASYLWNGGQTTSTISVNA
                     SGIYSVVAKNAYGCQSHAAKIQVNSLEPASCAKPDMLSAYNIIDKKASLMWNPAVTAD
                     SFRVAYNKMGSATVIVKYVRSRNNLDINELLPATTYQWSVTAICAGSSVVSATEVFTT
                     LNGPTDCGSTVVNTFTTNVTNS"
     assembly_gap    5928..6027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6028..6579
                     /locus_tag="PKY09_11090"
     CDS             <6028..6579
                     /locus_tag="PKY09_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNB13442.1"
                     /translation="CDVISGITISNVASNKATVSWNPQSGVRYVLIRYAPATSNQWKY
                     IYANASTGNKVINNLTPGVAYKVQLKSMCTYSNNSAYSAMERFTTGIARLGEATSELM
                     INAYPNPADAHITYAFDAKKEGYFFVRVSDIAGRILLNDKKMASEGVNGGDIDLRLFT
                     PGVYTLTITKGSEENHIRFEVNR"
     gene            6685..7401
                     /locus_tag="PKY09_11095"
     CDS             6685..7401
                     /locus_tag="PKY09_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13443.1"
                     /translation="MKLWSFKICVIAILYFFVDVLQAQTIHVVASVKGNDATLPVVGL
                     MIVNKSTGTGFFSPDNNFFSAAFNKSDTIMVNATGYSVKKLCFNDSLIKDTFRIAIIL
                     EKPIVTLKPVTIIPPRELEVIQKDIEKLGYKKEDYMVTGIDAFESPITFLYQSFSKRE
                     RAKRDIAQKKNEDNKRKLLKELFRKYVDFDIMNLSEDQFDNFIDFINVDDNFLKNSTQ
                     YDFILFVKRKFEMYKALKKQ"
     gene            complement(7414..7710)
                     /locus_tag="PKY09_11100"
     CDS             complement(7414..7710)
                     /locus_tag="PKY09_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNB13444.1"
                     /translation="MFGKLAEAQKKMQEIKDRLETVIVTGESEQGKVVAEANGNNRIR
                     SITINESLFKIGDKNEVEELITLAVNRALEASEKLMQIEMAAASKDILPGFLQK"
     gene            7877..8437
                     /locus_tag="PKY09_11105"
     CDS             7877..8437
                     /locus_tag="PKY09_11105"
                     /inference="COORDINATES: protein motif:HMM:NF017816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HNB13445.1"
                     /translation="MRFLTVFVFCFVLSQTTNAQRELIEWNDSISFKIEDFKGPITSP
                     DKNLHISTNCGLYCIPQIVGDTAILTIIAYMDKNKSWARKKDVTPALLEHEKGHFDLT
                     EVFARKMKKRVLALRTNKRTFVKDVQEVYNKTWEDLQKQHQAYDKATEFSNVEFAQRT
                     WQNYIKEHLVQYHFYASQTIRFPIKD"
     gene            complement(8454..>8661)
                     /locus_tag="PKY09_11110"
     CDS             complement(8454..>8661)
                     /locus_tag="PKY09_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13446.1"
                     /translation="MTTPFPILAPNIRSKKHRIALDGQKGLMKKITFTQCQRKNFNED
                     APRSNSDGLNLDKSDFTRSSIGQK"
BASE COUNT         2760 a         1618 c         1665 g         2518 t
ORIGIN      
        1 tacttattca tccatagatt aatcccagtg attggtgatt tgtgatttta atcactcaaa
       61 atttgaaaag taattccgga atcttacatc attgaaaaca ggattttgca tttcgatgaa
      121 tgttgcttta agagggaagg aaacaaatga tgacctgaaa tatttgccaa cataaatcgt
      181 tttcttgcaa atgcaataat gaaagctact tgaatgctat caggtaaaac agcagtttat
      241 attgcctgcg ttggcacgag aaaaaaagtc aaaacgaaca aaagcttctt ttcaaacaac
      301 gtgccaaaaa tttgttgaac caaaaaaaaa ttcaaatggc acaaaaaatc tacttcgcaa
      361 atgctgtcag cgaaatgaca acagaaatca ggggagtaat taacaaacag ttcagaaaat
      421 tcaagattct gagcatttcg ttattattca tccatttact ttttgcagca ttatcattgc
      481 ttcaggttgc taatgcgcaa acttatgcac cggtaagcta ctggacattt aacagcagcc
      541 cttttaataa ggactcaatg aatcgtacaa atatggactt tacttcatat ggttgcggtt
      601 acactgtttc taataacagt gctgttggca aaggtttaat tttaggtagt agtggtgata
      661 atattaatac tggcaatttt gtaacagata catcatttgc cattgaattt ttatttaaac
      721 ccggatatca attcaatcag acattgctgt tcagaagaat ggatggcggt ttttctgcta
      781 attttaattt tgacggaata tacttctata cctatacctc cggtagtggt tcagacgaac
      841 tgaaagtaag ttttgatgga ataggtaaac gttcctatgg atattttgtt gatggcaact
      901 ggcatcatat cgtatttcaa tacagttcgc ttaccggaaa aaaagaaata tatattgatg
      961 gcgaatgtcc ttcaggatat tcaaaaaata tttcaggtaa ggttgtgaat ggttcaaaca
     1021 caagtttatt ttggaatcag ggagtaagtt atatgaaata ttttggtgag tatgatgaaa
     1081 ttgccatata caacagaact gtccctgcta agttgattta tcagcattat aaagatgctg
     1141 tacaaacaaa taaaagctat agttttaatc tgacatttaa tggcagtttg ccttcgccat
     1201 ctccaatatc agccggttta gataatacag aatttgctcc cggacatcca aatcctaacg
     1261 tttccgccat tgatcagttg agaagctatc ctgtagcccg ttataaagcc ggtaatacat
     1321 tgatgaaaaa tttcaactgg gttgacctta tgtacatggg aggccgtttt cagagtggtg
     1381 tatctgatgc acagtcagca acaaacagta ctgcaataaa cgaagagctt gctaaaaact
     1441 ggaattacat gattcagctg cgactcagca tggatcagtg gggaaatgca tgggctaatc
     1501 tggctaatca gaacccacaa ttaggaacat cattaattac acttcgtgca cagttaaatc
     1561 cttcattaat cagaagtcag aatttatcca gcgacaagta tcttcaaaat tcttcaggaa
     1621 actttattga tccaaacggt aacaactctt caaccaaatg gtggagacca acagcaccgg
     1681 caaatgctta ttccggtgat ggtaatcaaa tgaaatcaaa tgttcaggat gtattgaatg
     1741 cattgacaag accattaaca atgattaatg agaatggtga agtgtttgcc tggagtactg
     1801 atgctgcatt atctgctgac cctgccgttg ttgctgcaaa aaatgcttcc ggtttggatt
     1861 ttcaaacttt tcaggcaaga aaatttaaag aaaacgaaac acaatcatac cgtgatataa
     1921 tcatggatat tcctgcgctg tcaaatacaa aatttacaga gtatggtatt gatggttttc
     1981 cacaatatcg tcagaaatat agtgaagcac gtttggtaaa ttctgaaatc aatggtcagc
     2041 gttatagcac accagatttt tatccccgtt ggccacacaa ctggcgtaac tggataacgg
     2101 catggcatgg ctggcagtgg attgttgaca gtcgttatta tgaattacag gcgggtgata
     2161 atttgttctc tccatttgtt gcagccggtt gggatgttaa tgaagataac aatatgcgtc
     2221 ctgcacagtt tatgggattg ttgaaatgcc ttaacatgat tggtgcagag tttttctata
     2281 caggttattt taacgaagcc ggaaattaca acccaccaaa tcctccaccg gcaaaccctg
     2341 caggttatgc atggcaaaca gcaatgccaa gttatgcaca agcagttgcg agtcgctatg
     2401 aagaattctt gcgtaatggt agtgtaatgc caggtgatgt acctgcatat tacagtgctc
     2461 catcaggtaa accgggatat acattctggg caggtgactt acgtaagtta gttgtagtta
     2521 gaaaacataa tacacaatct aaatttgcta ttaccggaac tattcagcca aacacaagta
     2581 tggtaggtgg tgcagagaat gaaagtgttg caacaattac acttgatggt caacaagtaa
     2641 aattcaaagt aagacgtcag ggcagtactt atatttatga taaaaccaat gcatcatctc
     2701 ctgtatttta tcagttagat gagtggcatg aaaaatcgca tccatcattc tggtcaaaag
     2761 attttactat tgagtcagaa ttgtatgata ataccaatgc agcatataat ttaaagacta
     2821 ttcaacctca aggtgctgct gctggcgatt attctaactt tactactgca gttgtgtttc
     2881 cttcaacaca aacaactttc actccattag agtatgtatt tcagccaaga agttcatcac
     2941 aggctaactt ataccttttt gtgcgtgcaa gaagtactga tggcactgca acaggtttag
     3001 gtgtttctgt tgacaatgga gcatcaaaat ctatcggttg cgtaacagat acgaactgga
     3061 catggtatcg tttagatgca tgttcacaac aggcaataca gtttacaggt ctaagcattg
     3121 gaagtcatac tattcgtttt gctccaacaa ataataagat aatgattgat aaattcattt
     3181 tatcagcaaa caataatgta atcacaaact attcaatggt tgcctgcaat tccggtggaa
     3241 cagccactat taatgcaagt gggccaacaa cattctgtca gggtggtagc gtaacattaa
     3301 ctgcaggtgc aggtaattca tacttatggt caaatggtgc aactacacag tcaatcactg
     3361 tttcacaagc aggaaattat gtagttacag tttcacaggg aagtggatgt gctgcaatca
     3421 gcaatccggt agcagtttct gtaagttctg ctcctgcagc tacaatttct gcaaatgggt
     3481 caacttcatt gtgtcagggt gcttctgtta cgttaactgc aagtgcaggt tcaacttatc
     3541 aatggtcgaa tggtgctact tcgcaatcaa taactgttaa cgcagcaggc aattacgtag
     3601 taactgtcag caatgctcag ggttgctcgg caacctctgc tgcaacaaat gttaccatga
     3661 cagcaggtgg aacagctacc attacagccg gtggtcctac aacattctgc ccaggtggta
     3721 atgtaacatt aactgccaac accggaacat catacttatg gtcaaccggt gcaacaacaa
     3781 aatcaataac tgttaatgca acaggttcat attctgttac agtaaacaat ggatcatgca
     3841 gtgcaacatc ggcaccaact tccgtaacag ttagtaattc agcaacggca actatcacag
     3901 ctaacgggcc aacaacattc tgtccgggta gcaatgttac attaactgca aatgcaggat
     3961 catcctatca atggtcaaac ggtgcaacca ctcaatctat aaccgtaggt gcagcagggt
     4021 catacgctgt aactgtatct aatggaaatt gttcagcaac atctgctcca actgttgtaa
     4081 ctgtaagcaa taatgcaaca gcaactatca ctgcaggagg tccaacatca ttctgtcagg
     4141 gtggaagtgt tacattaact gcaaatacag gttcgtcata tctttggtct aatggtgcaa
     4201 caacaaaatc tatcaccgta aatgcatcag gttcttattc agttacggtc tctacaggta
     4261 gttgttctgc aacttcagct ccaaaagttg ttacggtgac gcaggctcct aatgcaacca
     4321 ttacagccgg aggcccaaca tcattctgtc agggtggtag tgtaacatta accagcagca
     4381 caggaacttc atacctttgg tcaaacggtg caacaacaaa atcaattacg gttagcgcag
     4441 caggttcata cgttgtaacg gtatcaaacg gtagctgttc agctgtatca gcaccacaaa
     4501 ctgttgcagt agcagcaaat ccaacagcaa caattacacc taatggttct accatgcttt
     4561 gttcaggagc aagtgtagta ttgaaagcaa gcagtggcac ctcatattta tggtcaaccg
     4621 gtgcaacaac acaatctatc actgtttcaa ctgcaggaag ctatacagta aaagtttata
     4681 atgcacaagg ttgtgtaact acatcagcac ctattgcaat aactacagga agtgcatctg
     4741 caacaattat tgccaatggc ccaacaactt tttgtcaggg tggtggcgta acattaagtg
     4801 caggtgccgg aacatcttat caatggtcaa atggagcaac tactcaggat attaatgtga
     4861 gcacttcagg aagttatgtt gtaacagttt ccaatggttc ttgttcggca acgtcagcac
     4921 cgttagtgat aaccgttaat ccattgcctg cagcaactat tacagcaagt ggttcaacta
     4981 ccatcacaca aggtcagtca gtaacattaa ctgcatcttc cggcagcagc tatctatggt
     5041 caaccggtgc aacaacttca tcaatcaccg taaacacagc aggaatctat accgtaaaag
     5101 ttaccaatgc acagggatgt tctgcaactt catcgccaat ggttataact gttgtaaaca
     5161 actcacaacc tgcatcaata actactactg ctgccaacaa tcagatttgt atgggacaat
     5221 ctgctacact tacttcaagt cccggtatgc aatacatctg gttgcccgga ttacaagcaa
     5281 cacaaagtat tgttgttaat gcaccgggta catacactgt aaacatcaga aacagtgacg
     5341 gttctacatc tactgctaat atcaccatca gtcaggcacc aacgcctaat aagcctgaag
     5401 ttacctacac ttatgttaca ggtacaatgt atcagctaaa agcgtttgag ccaagtgcag
     5461 catcttatct gtggaacggt ggtcagacaa cttcaacaat atctgtaaat gcatcaggaa
     5521 tctactcagt agttgctaag aatgcttatg gatgccaatc acatgcagca aaaattcagg
     5581 ttaactcatt agagcctgca tcatgtgcta agcctgatat gttgagtgcc tataacatta
     5641 ttgataaaaa agcttctttg atgtggaatc ctgctgtaac tgccgattca ttcagagtgg
     5701 catataacaa aatgggaagc gcaacagtaa ttgtaaaata tgtacgttca cgtaacaatc
     5761 ttgatataaa tgaattgtta cctgcaacta catatcagtg gagcgttact gctatctgtg
     5821 caggaagctc agttgtaagt gcaacggaag tatttactac ccttaatgga ccaacagatt
     5881 gtggcagtac tgtagtaaat acttttacta ccaatgttac caatagtnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnntgt gatgttataa gtggtataac tatatctaat
     6061 gttgcgtcaa acaaagctac tgtaagctgg aatccacaat caggtgttcg ttatgtgtta
     6121 atccgatatg cgccagccac aagcaatcaa tggaaatata tatatgcgaa tgcatcaaca
     6181 ggaaataagg ttatcaataa tttaacgccc ggtgttgctt ataaagtaca gttaaaatcc
     6241 atgtgcactt attcaaacaa ttcagcatat agcgcaatgg agcgtttcac aacaggtatt
     6301 gcacgattgg gcgaagctac ttctgaactg atgattaatg cataccctaa tcctgcagat
     6361 gctcacatta cttatgcgtt tgatgcaaag aaagaaggtt atttctttgt gagagtcagt
     6421 gatattgcag gtcgaatttt attaaacgat aaaaagatgg cttctgaagg agttaatggt
     6481 ggcgacattg atttgagact gtttactccc ggtgtttata cacttactat cacaaaaggt
     6541 agtgaagaga atcatataag atttgaagtg aatcgctaaa aacagaaaca gtaacttcaa
     6601 aatgagagga agtccaaacg ggcttcctct tttttatgtg taatcttcat gcagatgtta
     6661 gcaggaagtt attatcattg ccctatgaag ttatggagtt ttaaaatttg tgtaatagcc
     6721 atcttatatt tttttgttga tgtactacag gcgcaaacca ttcatgtagt tgcaagtgta
     6781 aaaggcaatg atgcaacatt gcctgtagta gggttgatga ttgtaaataa atctaccggg
     6841 acaggctttt tttcacctga taataatttt ttttctgctg cattcaataa gtcagacacg
     6901 attatggtaa atgccacagg ctattcagtg aaaaaattat gctttaatga ttcattgatc
     6961 aaagacactt tcagaatagc tatcatactc gaaaaaccca ttgttacact caagcctgta
     7021 acaataattc ctcctcgcga acttgaagta attcagaaag acattgaaaa gttaggttac
     7081 aaaaaagaag attatatggt taccggcatt gatgcatttg aaagcccgat tactttttta
     7141 tatcagagtt tcagtaagag agagagagca aaacgagaca ttgcacagaa gaaaaacgaa
     7201 gacaataaga gaaaactact caaagagtta ttcaggaaat atgtggattt tgatattatg
     7261 aatctaagtg aagatcagtt tgacaacttt attgacttta ttaatgttga cgataatttc
     7321 ctgaaaaact caacacaata tgattttata ctattcgtaa aaaggaaatt cgaaatgtat
     7381 aaagcgttga aaaaacagta aattgtaaag tcattacttc tgcaaaaatc ccggcagaat
     7441 atcttttgat gctgctgcca tttctatttg cattaacttt tctgaagctt caagcgcacg
     7501 gttaacagca agagtaatca actcttcaac ttcgttttta tcacctattt taaacaacga
     7561 ttcattgatg gttattgaac ggatgcggtt attgccgttg gcttcggcta ccaccttgcc
     7621 ttgttccgat tctccggtga caattactgt ttctaaccgg tcttttattt cctgcatttt
     7681 cttttgcgcc tcagctaact ttccaaacat atttattcag attttattta tggcaaagtt
     7741 aatcatacac aataattttc actaaaaaca gcctttaaat ttttaaaaaa ctgtaaggac
     7801 aatgtaaggt ttgtggagtt attagtgtag aataaaccgg agaactgccg tataagaatc
     7861 taaaatttta gaaagtatgc gctttttgac tgtatttgtt ttttgtttcg ttttaagtca
     7921 gacaaccaat gcccaacgcg aattgataga gtggaacgat tccatatcat ttaaaattga
     7981 agattttaaa gggccaataa catcacccga taaaaacctg catatttcaa ccaactgtgg
     8041 cttgtattgt attccacaga ttgttggcga tacagctatt ctcaccatca tagcatacat
     8101 ggataagaat aaatcgtggg cgcgaaaaaa agatgttaca ccagctttgc tcgaacatga
     8161 aaaaggtcat tttgacttaa cagaggtgtt tgcccgtaaa atgaaaaaac gcgtattggc
     8221 attaaggacc aataaacgaa ccttcgtaaa agatgtgcag gaagtatata acaaaacatg
     8281 ggaagacttg caaaagcaac atcaggctta cgataaagca acagagtttt ccaatgttga
     8341 atttgctcag cgtacctggc agaattatat taaagaacat ttagtgcagt atcattttta
     8401 tgccagccag actattcgct ttccgataaa ggactaacac atggttacat tttttatttt
     8461 tggccaatgg aagaacgtgt aaagtccgat ttatctaaat ttaatccatc agaatttgac
     8521 cgtggcgcat cttcattaaa attttttctt tggcattggg tgaatgttat ttttttcatc
     8581 agcccctttt gtccatcgag tgcaatacga tgttttttgc tgcgtatgtt tggagccagg
     8641 atagggaagg gtgtagtcat c
//
LOCUS       DAOQNP010000096         8637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3018_length_8637_cov_8.982638, whole genome shotgun sequence.
ACCESSION   DAOQNP010000096 DAOQNP010000000
VERSION     DAOQNP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3018_length_8637_cov_8.982638"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..492)
                     /locus_tag="PKY09_11115"
     CDS             complement(226..492)
                     /locus_tag="PKY09_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13447.1"
                     /translation="MKKVMIAAAALIIGGTAFANAQTNAKPAATTTHQVAPATTHETA
                     AQKKEVKEEKMEKKEATATKHEAKKPAAHKSHAKKTEKKEESKK"
     gene            complement(977..1411)
                     /locus_tag="PKY09_11120"
     CDS             complement(977..1411)
                     /locus_tag="PKY09_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13448.1"
                     /translation="MKRIMLLAGLCLALTATMRAQVVQKRETSDQAQQQLLQLNDEMA
                     ASFHHTDLLKVSEYFDDDATIYIDGNKVTGRKALSEFWNKITARKKMEISVDEMNGNG
                     KFIYETGTLTMQTESNTVNKNFVMVWKRLPNFDYKIVVAGFN"
     gene            complement(1515..3215)
                     /locus_tag="PKY09_11125"
     CDS             complement(1515..3215)
                     /locus_tag="PKY09_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNB13449.1"
                     /translation="MNKTVTVNIGGIVFHIEENAYDMLGSYLQAIRNHFTISDGRDEI
                     IQDIESRIAEIFQQNLGTSRQVVTADDVNQAIGIMGRPEDFGATEEASANNASASTEQ
                     NNQSDHFFRRRLYRDTDDKVIAGVCSGIGHRLGIDAVWMRLIFIIIFFFAGSGILIYI
                     ILAIVMPKAITPTQKLEMRGDPVNISSIRRETETPASAKQPSAVSGVFDTLGDLIKGV
                     FKFVVYAIMAILAFAGLMILIGIGLFILAMLGVSGLTIPIYISDQFLSSSQQWWVSLS
                     VLLLIGVPAIWLMTLLIKWIFRIKYNSRFLNVTAGMLFMLGIVIAFFIGLNIARDFAF
                     VGKVRNTVELITPASSTMYLKLMDDPKYDEEDNSKIFHRRGSNFSITTGSNFEFNSDD
                     VSFTVQKSPSDKFELIRVYAANGATENLAVESARAIIYNFEQKDSLLMFSDRFPIGKG
                     TKYRAQQVNLILKVPVGSSIFLDESMEEIIHDIPNVTDTWDGDMGGHNWKMTENGLVC
                     TDFEFQKGDSTDIHNPENVDISIDNNGVRIKGNDAKSGDKDVNINIGKDGVHIKSTDK
                     "
     gene            complement(3212..3523)
                     /locus_tag="PKY09_11130"
     CDS             complement(3212..3523)
                     /locus_tag="PKY09_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNB13450.1"
                     /translation="MRKGVLEFCILSIISKGEVYPSDIIEKMKEARLIVVEGTLYPLL
                     TRLKNSGLLSYTWIESNAGPPRKYYKLTPLGKEHLKELRKTWDELVDAVSQTYQNSTE
                     E"
     gene            complement(3811..5394)
                     /gene="ftsZ"
                     /locus_tag="PKY09_11135"
     CDS             complement(3811..5394)
                     /gene="ftsZ"
                     /locus_tag="PKY09_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNB13451.1"
                     /translation="MPKELPSIIKVIGVGGGGSNAVNHMYKLGIRGVDFVVCNTDAQA
                     LEMSPVPRKIQLGPSLTEGRGAGSIPEVGKQSAIENIDELKAILSDNTKMVFITAGMG
                     GGTGTGAAPIIAGVAREMGILTVGIVTVPFAFEGRKRKQQADAGIEELKNSVDTLLII
                     CNDKLREIHGNLKLNTAFGYADNVLATAAKSIAEVISVTLHINVDFADVQTVMRDSGV
                     AIMGAAQSSGEGRAIRAAELALDSPLLNDNNIEGARHILLNITSGKEEITMDEMGDIT
                     DYIQQKAGQTAEIIMGVGTDDSLGDKISVTIIATGFNANKEKAKEEKVKQPEKIILKM
                     YDETPTSVVNEVTDTHVEEKPVVNEPVLITLEQEIPVTSTEFFEKDNTPEEIVADNST
                     MEFEFTGFPNVISQNEEVVEPEATLAEDPTVSELPKQAEIAVPEVTSQDNRTPDSDIY
                     KRTRERIDAMKSMNHRISNPQALNEIEKEPAFLRKNVKLQNVLSSGDTNYSRYSLNGD
                     QDKTPGLSKGNKFLHDRPD"
     gene            complement(5449..6744)
                     /gene="ftsA"
                     /locus_tag="PKY09_11140"
     CDS             complement(5449..6744)
                     /gene="ftsA"
                     /locus_tag="PKY09_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNB13452.1"
                     /translation="MAEQVTGNEVIVGLDIGTTKIAAIVGRRNEFGKIEIVGTGRAES
                     VGVTRGVVANIEKTVGSIKQAVAEAEHKCGGNIKSVHVGIAGQHIKSLQHRGMITRSS
                     TDDEISQRDIDALIDDMYKLAMPPGEEIIHVIPQEFIVDNEQGIKDPIGMSGIRLEAN
                     CHIITGLVTAANNIHKCVTKAGLTPADLILEPLASAEAVLTEEEKEAGVVLVDIGGGT
                     TDVAIFQDNIIRHTAVIPFGGNVITEDIKEGCSIIRNQAELLKIKFGSAFASQMKDNE
                     IVSIPGLRGREPKEISVKNLASIIQARVEEIIEQVYYEIKTSGFEKKLIAGIVVTGGG
                     AQLRHITQLMEYVIGMDTRTGYPNEHLAKGAEETKSPIYATGVGLVIKGFQDLDKRKV
                     AGRSAGTTEPRVQHKGSWFSGIFDSTKKIFTDDSDDKII"
     gene            complement(6757..7602)
                     /locus_tag="PKY09_11145"
     CDS             complement(6757..7602)
                     /locus_tag="PKY09_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13453.1"
                     /translation="MKTNFKINDKLRSAFLIAGYVLVAATVLTLMSFAAKRNHELICK
                     GVVVNIEDENVKGFIDRSDIMEIVRSKGKKIIGNKVADINTFMLEKIIDANPHVLKAE
                     VYSTIDGWVHIDVQQRTPIVRIINSKGENFYIDNQGKFMPLSEKYSEPVMVASGQIAE
                     GLVQQKLGLDVNGLPLPDDSLGKLPMLSQVFLLAEHLKRDSSWDAMFEQIYVDENGEI
                     ELIPRLGNNYILLGNIANLDEKLNLLLNLYRQGFPATGWDQYESINLKFKGQVVCKKK
                     NVKLS"
     gene            complement(7599..>8637)
                     /locus_tag="PKY09_11150"
     CDS             complement(7599..>8637)
                     /locus_tag="PKY09_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNB13454.1"
                     /translation="TTTSSILAHILKTANVNVAAFLGGIARNYNSNIILPVSEHDVIN
                     VVEADEFDRSFLTLFPFAAAITSMDADHLDIYGSGEELKKTYQQFASQVDEKGLLVLR
                     SGLQLQPVKAMTKFYAAGAQAEFYADKISIRNHRYCFNFHFPDGVIDNLSLGLPGRHN
                     VENAVAAIALAMKVGVKEEDVRAALQSYKGVKRRFDIRYADVNCTFIDDYAHHPEELR
                     AAIMSVRELFEGKKITGIFQPHLFSRTRDFADEFAASLALLDEVVLLDIYPARELPLE
                     GITSSWLMNKIKHDNKILLGKTDVLDYINKHKPEVLITLGAGDIDTLVTPIQQLLEKK
                     YSVHELKNHRQ"
BASE COUNT         2443 a         1837 c         1501 g         2856 t
ORIGIN      
        1 attttcaaaa aaatattttg gcatgggtaa caaaacgcac attttcttct atttaagcag
       61 ttgcaccggc tattcatgca gttgtgttaa aacacactgt attttcaatg caaatgagcg
      121 attatatatt tgaagaaacc aaaaagagca ctttacacat tattatcaaa acaaaatgcc
      181 cgacaaactt tcgtatgccg ggcattgaac tttcaaaatc aatttttact ttttactctc
      241 ttcctttttc tcagtctttt ttgcatgact tttatgagca gccggttttt tggcttcatg
      301 ctttgttgca gtagcttctt ttttctccat cttctcttct tttacctctt ttttctgagc
      361 agcagtttca tgtgttgtgg caggtgcaac ctgatgtgtt gttgtagctg caggttttgc
      421 attagtttgc gcattggcaa aagcagtgcc tccaattata agggcagctg cggcaatcat
      481 tacttttttc attttattat ttgttttgaa atgatgggtc aaatgttcaa ttgccaacat
      541 gatgatgcta tgaaaattta tacatcttca ttttaactaa aaacagactt cataattccg
      601 tcatcggatt actaatggtt aatcaggtcg ccctgaagag gattttatta caaagatttt
      661 tatagttcaa ccaaaactgt acactcctgc aaagctttat agaataatta aaacaagtct
      721 tgtcacccat gagctttttg aaatgtaaac atggctcact tacgtactta ttaatgacat
      781 gttgaaatac tcacagaatc gaactcatgg caaaaagatg taaagaataa ttttaatagt
      841 cattgaataa cggcaaaaaa atattgtcaa gattatactc tgtaaaaaca gtgactaaaa
      901 acaacaagag gcctgctaat aagaagcaga cccccgattg tacaaacaca aattatcagt
      961 tactcaaact aattttttaa ttaaaccctg ctacaacaat tttataatcg aagttgggca
     1021 ggcgtttcca aaccattaca aagtttttgt ttacggtatt actttctgtt tgcattgtaa
     1081 gtgttcctgt ttcataaata aatttgccgt taccattcat ttcgtcaaca ctaatttcca
     1141 ttttttttct ggctgtaatt ttattccaga attcagacaa tgcctttcgg cctgttactt
     1201 tatttccgtc tatataaatt gtggcatcgt catcaaagta ttcactcact tttagtaagt
     1261 ctgtatgatg aaatgacgct gccatttcat cattaagctg cagcaactgt tgttgtgcct
     1321 gatcgcttgt ttctcttttc tgaaccacct gtgcgcgcat tgtcgcagta agtgcaaggc
     1381 atagccctgc caataacata attcgcttca taatatttga tttaaaattt aattgatatt
     1441 gattgataaa gggattaacc tttatgagta ggacgattac taaccggaat tgttacagtg
     1501 tgtaatgcag tattttattt atcagttgac ttaatatgca caccgtcttt tccgatgttg
     1561 atattaacat ctttatcacc gctttttgca tcattgcctt ttatgcgaac accattgtta
     1621 tctatactga tgtccacatt ttcaggatta tgaatatctg tgctgtcacc tttctgaaac
     1681 tcaaaatctg tacataccaa tccgttttct gtcattttcc agttatgtcc gcccatgtcg
     1741 ccatcccagg tgtcggttac attaggaata tcatgaataa tttcctccat actttcatcc
     1801 agaaagatgc tgcttccgac aggaactttt agaatgaggt tcacttgctg tgcgcggtac
     1861 ttggttcctt ttcctatcgg aaaacggtcg ctaaacatga gtaaagaatc tttctgctcg
     1921 aagttgtaaa tgatagcacg tgcactttca acagcaagat tctctgttgc gccattggca
     1981 gcatatactc ttatcaattc aaacttatct gagggcgatt tttgaaccgt aaacgataca
     2041 tcatcagaat taaactcaaa gttacttccg gtggtgatgc taaaattact gcctctgcga
     2101 tgaaaaattt tactgttatc ttcttcatca tatttaggat cgtccatcag ctttagatac
     2161 attgttgatg aagcaggtgt tatcaattca acggtattgc gcaccttacc aacgaaagca
     2221 aaatcgcgtg caatatttag tccgataaaa aatgcaatga ctattccaag cataaacaac
     2281 atacctgctg ttacatttaa aaaacggctg ttatatttga ttctgaaaat ccatttaatg
     2341 agtagggtca ttaaccagat ggcaggaaca ccgatgagca gcaacacgga tagagatacc
     2401 caccactgtt gcgagcttga cagaaactga tcagaaatat aaattggaat agtgagtccg
     2461 ctaacgccta acattgccaa aataaacaag cctattccaa tcaaaatcat aagtcctgca
     2521 aaagctaata tggccatgat ggcatatacc acaaacttga aaactccttt aatcaaatcg
     2581 ccaagtgtat caaatacacc tgaaactgct gaaggctgtt ttgcagaagc cggtgtttct
     2641 gtttcgcgtc tgatgcttga aatattcacc gggtcaccac gcatttcaag tttctgtgtt
     2701 ggtgtaatgg ctttgggcat gacaatggcc aggatgatat aaattaaaat accactgcct
     2761 gcaaaaaaga aaatgatgat aaagatcaaa cgcatccaca ctgcgtcaat gcccaaacgg
     2821 tggcctatac cggagcaaac acctgcaata actttatcat cagtatcgcg atacaacctg
     2881 cgtctgaaaa aatgatcgga ctgattgttc tgttcagtac ttgctgatgc attgtttgct
     2941 gatgcttctt ctgttgcacc aaaatcttcg ggacgtccca taattccgat tgcctgattc
     3001 acatcatcag cagtaaccac ctgccttgat gtgcctaagt tttgctgaaa tatttcagct
     3061 atacggcttt caatgtcctg aataatttca tcgcgtccat cgctgatggt aaaatgattg
     3121 cgaattgcct gcagataact gccaagcatg tcataggcgt tttcttctat atggaaaact
     3181 atgcctccga tgtttactgt tactgtcttg ttcattcttc agttgagttt tggtaggtct
     3241 ggctaacagc gtcaaccagt tcgtcccatg tttttcttaa ttcttttaaa tgttcctttc
     3301 ctaatggagt tagtttgtaa tatttgcggg gaggtcctgc attgctttct atccaggtgt
     3361 aggataacag ccctgaattt tttaaccgtg taagcaatgg ataaagtgtt ccctccacca
     3421 caatgagcct ggcttctttc attttttcga tgatatcgct gggataaact tctccttttg
     3481 atatgattga taaaatgcag aactccaaaa ctcctttgcg catttgcgac tgtgtgtttt
     3541 cgagattacc cacgtttttt agatttatga ttgtttaact ttattctttt actaaatgac
     3601 tatgcaaaga tatatatgtt aaatagtacc atgcaacaca tagtactaaa atattttata
     3661 ttacactgat tatcaagtca attattttta atgagggctg aaatattatt gaaaagtgcg
     3721 atgtacgtat cacctcgtgc agcgtagtga gcataaaaaa agccggaaaa ggaaaatcct
     3781 gtccggctta aataaagttg ttattcagca ctaatcgggt ctgtcgtgca agaatttatt
     3841 gcccttgcta agacccggag ttttatcctg atcgccattg agtgaatagc gtgaatagtt
     3901 agtatcgcct gaagaaagaa cattctgcaa ctttacattc ttacgtaaga aagcaggttc
     3961 tttttcaatt tcattcagcg cttgtggatt cgaaattctg tgattcatgc ttttcattgc
     4021 atcaattctc tcgcgtgtac gtttgtaaat atcgctgtca ggtgttctgt tatcctgtga
     4081 tgtaacttca ggtacagcta tctccgcttg cttcggcaat tcgctaaccg ttggatcttc
     4141 agctaaggtt gcttcgggtt ctacgacctc ttcattctgg gaaattacat ttggaaatcc
     4201 tgtgaactca aattccatag tactattatc agcaacaatt tcctccggtg tattgtcttt
     4261 ttcaaaaaac tctgttgatg taacgggtat ctcctgctct aaagtaataa gaacaggctc
     4321 attcactact ggcttttcct caacatgagt atcagtcact tcgttaacaa cagaagttgg
     4381 tgtttcatca tacattttta gaataatctt ctccggttgt tttacttttt cttccttagc
     4441 tttttcttta ttagcattaa agcctgtggc aataatggtg accgatattt tatcgcctaa
     4501 tgaatcatca gtaccaacac ccataattat ctcagctgtt tgtccggctt tttgctgtat
     4561 ataatctgtt atatcgccca tttcatccat ggtaatttct tctttaccgc tggtgatgtt
     4621 caggagaata tgacgtgccc cttcaatatt gttatcattg agtaatggtg agtcgagtgc
     4681 aagttctgct gcacgaatgg ccctgccttc gccagaagat tgagctgcac ccatgatggc
     4741 aacaccactg tcgcgcataa cagtctgtac atcggcaaag tcaacgttga tgtgcaaggt
     4801 aacgctgata acttctgcaa tactcttagc agcagtagcc aacacattat ctgcataacc
     4861 aaatgctgta ttcaatttta agttgccatg aatttcgcgc aatttatcat tacagataat
     4921 caacaatgta tcaacactgt ttttcaattc ttcaatacct gcatctgcct gttgttttct
     4981 cttgcgacct tcaaatgcaa aaggaacagt aacaattcca acagtgagga tgcccatttc
     5041 gcgggcaaca cctgctatga ttggtgcagc acccgttccg gttccaccac ccatacctgc
     5101 tgtgataaaa accatcttgg tattatcact cagaattgct tttagttcat caatattttc
     5161 tattgcagac tgtttgccta cttcgggaat agatccggca ccgcgacctt cggtgaggct
     5221 tggtccgagt tgtattttgc gtggcacagg actcatctcc aatgcctgtg catcggtatt
     5281 gcaaactaca aaatcaaccc ctctgattcc gagtttatac atatggttta cggcattgct
     5341 tcctccaccg cctacaccaa taacttttat gattgatggc agctcctttg gcataaattc
     5401 aaagttcatc atgttaattt attttttgtt ggttataaaa atttttattt aaattatttt
     5461 atcgtcagag tcgtcagtaa atattttttt tgtggaatca aaaattccgg agaaccagga
     5521 tcctttatgt tgtacacgtg gttcagtagt acctgcggat ctgcctgcaa ctttacgttt
     5581 atccaaatcc tgaaaacctt taatcaccaa tcctactcct gttgcataaa tcgggctttt
     5641 ggtttcttca gcaccttttg ccaaatgttc gtttggatat cctgtgcgtg tatccatgcc
     5701 gattacgtat tccatcaact gcgtaatgtg gcgcagttgc gcaccaccac cggtgaccac
     5761 aattcctgca ataagttttt tctcaaagcc ggaagttttg atttcataat aaacctgttc
     5821 aataatttct tcaacacgag cctgaataat gcttgccaga ttttttactg aaatttcttt
     5881 gggctcacga ccgcgcaaac cgggtattga aacaatttca ttatctttca tctgacttgc
     5941 gaatgcagag ccaaatttta tttttaataa ttctgcctga ttgcggataa tactgcaacc
     6001 ttctttaata tcttctgtaa tgacattacc accaaatgga attacagctg tgtgacgaat
     6061 aatgttgtcc tgaaaaattg caacatcagt agtaccacca ccaatatcca ccaacacgac
     6121 acctgcttct ttttcttctt cggtgagaac tgcctctgca cttgccaaag gttcaagaat
     6181 taaatctgca ggtgtgagac cggctttggt tacacactta tggatattat ttgctgcagt
     6241 tactaatcct gtaatgatgt gacagttggc ttcaagtcta atacctgaca ttccgattgg
     6301 atcttttatc ccctgttcat tatccacaat aaattcctga ggaataacat gtataatttc
     6361 ttcacccgga ggcattgcca atttatacat gtcgtcaatc aatgcatcta tatcacgttg
     6421 gctgatttca tcatcagtac tgctacgtgt tatcatgcct ctgtgctgaa ggcttttgat
     6481 atgctgacct gcaattccaa catgtacaga ttttatattg cctccacatt tatgttctgc
     6541 ttcggcaacg gcttgcttta tactgcctac agttttttcg atattggcaa caacacctct
     6601 tgttacacca actgattctg ctctgcccgt gcctacaatt tctatttttc caaactcgtt
     6661 tctgcgaccc acaatagccg caatttttgt agtgcctata tcaagaccta ctattacttc
     6721 attacctgtt acctgctctg ccacttattg ttagttttat gatagtttaa catttttctt
     6781 tttacaaact acctgacctt taaatttcag gttgatggac tcatactgat cccagcctgt
     6841 tgccggaaat ccctgacggt ataaattgag aagaagattc agtttctcat caaggtttgc
     6901 tatgttgcca agcaaaatat aattatttcc caatcgggga atcaattcaa tttctccgtt
     6961 ctcatctaca taaatctgct caaacatggc atcccaggat gaatcacgct ttaaatgttc
     7021 agccaacaaa aaaacctgac tcagcatagg tagttttcct aaagaatcat ctggtaatgg
     7081 caggccattt acatccaaac caagcttctg ttgcaccaag ccttcagcaa tctgaccgct
     7141 tgcaaccatc acaggctcgg aatatttttc agataatggc ataaattttc cctgattgtc
     7201 tatataaaaa ttttctcctt ttgaattgat tatccgtact atcggtgttc gttgctgcac
     7261 gtcaatatgt acccatccat caatggttga atatacctca gcttttaaaa cgtgagggtt
     7321 tgcatcaatg attttttcca acataaaagt gttaatatca gctaccttat tgccaatgat
     7381 ttttttccct ttggaacgaa caatttccat gatgtcgctc cggtcaataa acccttttac
     7441 attttcatct tcgatgttta ccacaacacc cttacaaata agttcatggt ttcgttttgc
     7501 tgcaaaactc atcaatgtta acacagtggc tgcaaccaaa acatagcctg caattaaaaa
     7561 agcagaacgc agtttgtcgt taatcttaaa gttggttttc attgtctatg gttttttaat
     7621 tcgtgaactg aatacttttt ttcaagcaat tgctgaatgg gtgttaccaa cgtgtcaata
     7681 tcaccggcac ctaaggtaat gagcacttca ggtttgtgct tattgatgta atccaaaaca
     7741 tctgtcttgc ctaataatat tttattatcg tgcttaattt tattcatcag ccatgaagat
     7801 gttattcctt ccaacggtaa ctcacgtgca ggataaatat caagaagtac tacttcatca
     7861 agtaaagcca gacttgcagc aaactcatca gcaaaatcac gtgtgcgcga aaataaatgc
     7921 ggttgaaaga tgccggtaat ttttttcccc tcaaacaatt cgcgtactga catgatagca
     7981 gcacgcagtt cttcaggatg atgtgcatag tcatcaatga aagtgcagtt aacgtcagcg
     8041 tatctgatgt cgaatctgcg ttttacacct ttataacttt gcagtgccgc acggacatct
     8101 tcttctttaa caccaacttt cattgccaga gcaattgccg caactgcatt ttcaacatta
     8161 tgacgaccgg gaagccctaa agaaagattg tcaataacac catcagggaa atgaaagtta
     8221 aaacaataac gatgattacg aattgaaatt ttatcggcat aaaattcggc ctgtgcacct
     8281 gcagcataga acttcgtcat tgccttaacc ggttgcagct gcaatccgct tcgcaaaaca
     8341 agcaaacctt tctcatcaac ctgagatgca aactgctgat aggttttctt caactcttcg
     8401 ccactaccgt aaatatcaag atggtcggca tccatacttg tgatggcagc agcaaaagga
     8461 aacaaggtaa gaaaggagcg gtcaaattca tcggcttcta caacattgat aacatcatgc
     8521 tcgctgacag ggagaataat gttgctgtta taatttcttg caattccacc cagaaaagcg
     8581 gcaacattaa catttgctgt tttaagaata tgagccaata tgcttgatgt ggttgtt
//
LOCUS       DAOQNP010000097         8573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3040_length_8573_cov_9.796079, whole genome shotgun sequence.
ACCESSION   DAOQNP010000097 DAOQNP010000000
VERSION     DAOQNP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3040_length_8573_cov_9.796079"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1744)
                     /locus_tag="PKY09_11155"
     CDS             complement(113..1744)
                     /locus_tag="PKY09_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13455.1"
                     /translation="MKRLITWCILLTNYVCNAQQWQWAKHIGSGYQFYGEQAEAITDG
                     TNIYLVGSYGGTLYLPGDTLYSNGNNDIFISKFDASGNLIWAKTLGGNYLQPNNHEDA
                     RGVFDPTCNCIYVAGNFKNTANFGNGITLTSLGSETDNFLARMDTSGNFVWARWMGGK
                     GKERNPKINVNPTGRIYLITQTEDSAHFGNFFIPPGGGIVQYDSLGNCLSAEIKFTAP
                     ITGNTNGVFLDFMGTDLIIYGLYRSTPFQLDTVTLITQGNYDGFIARADSNGNVKWIK
                     TYGNAGVDYIQSITIDNQNNIYATGGFKDSINLGGINLYNNGIDILFAKFDGNGNTIW
                     AKQAFATGASSFGNNINGDVDGNCYVVGNFAGDITLDGFHISTANSNDMFLTRFSNNG
                     DFLGVRHFGQAGGFNSVVDNAGSVYNAGVFYNTIAIGSTSMTALLGQDIYLAKIDAIT
                     GIGGGEGRMANNQLLIYANPNQGKCNITVPDDFVNETNLILSIYDNSGKLIQQKTSEM
                     NEGNIKLNLEQEAKGVYNVVLSNKKKSYNGKIVFE"
     gene            complement(1759..2352)
                     /locus_tag="PKY09_11160"
     CDS             complement(1759..2352)
                     /locus_tag="PKY09_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13456.1"
                     /translation="MKTETEIKAANAAFLVDGENIPVSMVKNIKGWNSVTVSNTIIDG
                     VSNITARKEININTEFHASSTSEVHIYTGETFAECPDYTGFARVAQNPANDSSSSKIS
                     PNKGIELTFVKNNRTKPDAEIIPNPATGLFVIKMLNVDDANLTAVRIKNMVGRQVRQV
                     TFAGSEYKMDMSHEARGIYMVEITTGKNRILKKLIIN"
     gene            complement(2349..3986)
                     /locus_tag="PKY09_11165"
     CDS             complement(2349..3986)
                     /locus_tag="PKY09_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13457.1"
                     /translation="MKKYFIFLILLVSTKLTSAQNWQWAKHIGSDCGSYNEKINSVIS
                     DGSNIYVIGKFYGCLYLPNDTLFSNGFDDIFISKFDGGGGEIWSKQIGSSNTNITYHE
                     NANAVFDPVNNCIYLSGTFINTIDFGNGIILNSYSTSNVDNFIARMDLNGNFIWAKKC
                     TGNGIKREPNIYVDSSGNIYLLTQTADSVYFDGFHVGPGGTIAKYDPNGICYYAQNKF
                     TAPVLGNTNAVFLNFIGSDIFLYGAFRSIPFQIDTALLIPQGNYDAFIARADSNGGIK
                     WIKTFGNAGLDYIQSLSIDNFNNIYIIGGFQDSIDFGNGNLYNTGKDIVIAKFDVNGN
                     LIWNKQLFVTGNLQSGNQILSDLDGNSYIAGLFSGFASFGAFNLFTSNASDMFLARYD
                     QNGNCMGVRNFGEASCQSISIDSNNNVICGGFFKNTVNIGGNTFTSYLSNDLFLAKID
                     AITGIGGRETNPNNQLIIYANPNAGKCNITVPDDFVNEKNLVLSIYDNTGKLIQQTTL
                     EMNEGKIKVNLEAEAKGVYNVTLSSKKKSYNGKIVFE"
     gene            complement(3999..6317)
                     /locus_tag="PKY09_11170"
     CDS             complement(3999..6317)
                     /locus_tag="PKY09_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNB13458.1"
                     /translation="MKKICLFIMVLSIACCYISIGQGTGYVTLKGRQFYDTAGLPFFP
                     MVMNYHVDIVHDQTSNPTTFKVTRSSAYGTMGCALEGGYTYADCENSLTQDFAKLHQM
                     GFNSIRLLLSPHRKNGSLGFTYKSNYFADMCNENMQYTFDFNSPYNSASNQTFFNLVA
                     HVLDIAENNNIKVIFLCSDGSSVGENDRGTSDIDALDYAQYLHVLASYLQNKPALLAY
                     DLYNEPHWNVYDHNLTQSKQKVCEYTGWWYDAIKTSDPNHLVTIGGTDMGDVMDWDGS
                     MMKVDFISMHLYPVYPDYENKDQAKATARVLDLISWCSSALQHPWIIGETGLSTSPDA
                     CINSYPGAADGEYTDQYNYIASILPAVRDCGGSGFSWWEFMDVHWYCIPSAICSTCTY
                     CNTCGGIPSTPKEIHGNYWGLLQYADPVNYYSTADKPAVNFTIAFDEGNGTPAPAPCA
                     APTANYLDPYNCGLFNTTHHNAVTGTLKDANANPIKGGVVQALSWLYLDDNQTPSNPN
                     DDEYVHTWFYYYADDNGDFEAIPYNEINIGDDRIIYIRGSAPVAEKFERGEAWGSINP
                     DGWTDAQIQPAILGNITLDKINFQYDGVFDNENIPVSTQKNIKGWNSITVSNTTIDGI
                     SDITAREEININTEFHASATSEVHIFPSDAFIDCDATSTYSRFASNNSVHEENQLTNH
                     NEIEIRFSKNKNCDFSIIPNPNNGTFSLEIYCNENNVATVSIKSILGKELQKFSSTKD
                     FISLNLHDFPKGIYFVEAIIDNNKIVKKIIIN"
     gene            complement(6325..8163)
                     /locus_tag="PKY09_11175"
     CDS             complement(6325..8163)
                     /locus_tag="PKY09_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNB13459.1"
                     /translation="MQATLQASSYNGYNISCYGMQDGTLAVNISNGTAPYNYTWSNGD
                     TLATVSSLAAGYYAVRVRDAAGGDTTLQITLTQPEQLAIAEMTPYYYANDFNVSTYGA
                     CNGSVTTLINGGVLPLTYHWEPGQQNILAPSNLCGRENTLTVTDLNGCKASSSINLKE
                     PQRDDWTMTGNWGSNATDNFIGTNDNKDLVFKTNNSERLRLLGNGDIKINSFTGTGNR
                     ILTSDANGKLIPGPIVVNPTQSCTDPYTFAWQIKTNILPNIAYTCWNQVGIGTDDPQK
                     NLGVKGGTRFFNFNDHTKYLDVEHDGANASINSTDNVLINYYSGKNIYLNTGTTKGDV
                     ETGGNTFLATATGRVGIGSTSPSQALEITHSDQYGGIAINQVSNQYYTSEIKFNYQGV
                     QKWATGCYLGSINNQSYFIWNHGLAIQRTAFFIDGTSNNTGIDTDVPTAKLDVNGDLR
                     VRSLSGNNGKLIELDANGNLVASNIASGNAGLWATDANGIYNIGKVFVGFNNCSDCSI
                     SPYKLYVEGGIMTRDVKVTALPFPDYVFAKDYKLMSIYELEEYLQLNKHLPEIPSAKE
                     IESNDGFELGDMQTKLLKKVEEQTLYIISLQKQVDELKELVYRTKK"
     gene            complement(8385..>8573)
                     /locus_tag="PKY09_11180"
     CDS             complement(8385..>8573)
                     /locus_tag="PKY09_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13460.1"
                     /translation="ILDIMRHDFKQHENKIEAHSSNPVTNLAAAHQSEREKLGSFERY
                     FKELRNEFQAFIHKEAFS"
BASE COUNT         2506 a         1748 c         1455 g         2864 t
ORIGIN      
        1 tgcattgcat acataacttg tcagtaaaat gcaccatgtt attaatcgtt tcattttttc
       61 tttacaacag attcgttcgt ataatcttaa agattcgtaa ctcgcagata acctactcaa
      121 acactatctt cccattataa cttttcttct tattactcag cacaacatta taaactccct
      181 ttgcctcctg ctccaaattc aattttatat taccttcatt catctccgat gttttctgct
      241 gtatcaattt tccgctgtta tcataaatgc ttaaaataag gtttgtctca ttcacaaaat
      301 catcaggcac ggttatgttg catttgcctt ggttggggtt agcataaata agcagttgat
      361 tatttgccat tctgccttct ccgcctccaa tgccggtgat ggcatcaatc tttgccaagt
      421 aaatatcctg ccctaaaagg gctgtcatgc tcgtgctacc aatagctatt gtattgtaaa
      481 agactccggc attataaact gaaccagcat tatctactac tgaattaaat cctcctgcct
      541 gaccaaaatg acgaacacct aaaaagtccc cattgttgct gaaacgagtt aaaaacatat
      601 cattactatt tgctgttgaa atatgaaacc catctaaggt tatatcaccg gcaaagtttc
      661 caaccacata gcaattgcca tcaacatcac cgttaatatt attaccaaat gaagaagctc
      721 ccgtagcaaa agcctgttta gcccaaatag tgttgccatt tccatcaaac tttgcgaata
      781 aaatgtctat tccattatta tataggttaa ttcctcctaa atttattgaa tccttaaaac
      841 ctcctgttgc ataaatatta ttttgattat caattgtaat actttgaata taatcaaccc
      901 ctgcattgcc ataggttttt atccatttta catttccatt actatctgcc cttgcaataa
      961 aaccatcata attcccctgc gtaattaacg ttactgtatc taattgaaat ggagttgaac
     1021 ggtacaagcc atatataatt aaatccgtac ccataaaatc taaaaaaact ccgttggtat
     1081 ttcctgtaat cggggcagta aattttattt cggccgaaag gcaatttcca agtgagtcat
     1141 attgcacaat acctcccccc gggggaataa aaaaattacc aaaatgagca gagtcttctg
     1201 tctgggttat aaggtatatt ctaccagttg ggttaacatt tattttagga tttctttctt
     1261 ttccttttcc tcccatccat cttgcccaaa caaaattacc gctggtgtcc atgcgtgcta
     1321 agaaattatc tgtttcagag cctaatgaag ttaatgttat tccattacca aagtttgcag
     1381 tatttttaaa gttacctgct acgtaaatgc aattacatgt tggatcaaaa actcctcttg
     1441 catcctcgtg gttattaggc tgtaaataat taccgcctaa cgtttttgcc caaataagat
     1501 ttcctgatgc atcaaactta gatataaaaa tatcgttatt cccattgcta taaagtgtat
     1561 cgcctggtaa atatagcgtt cccccatacg accccacaag ataaatattt gttccatctg
     1621 taattgcttc ggcttgttct ccataaaact ggtagccgct tcctatgtgt ttagcccatt
     1681 gccattgttg tgcattgcat acataattag tcagtaaaat gcaccatgtt attaatcgtt
     1741 tcattttttt gagttttttt aattgatgat taatttttta agtattctgt ttttaccggt
     1801 ggttatttca accatataaa ttccacgggc ttcatggctc atgtccattt tatattcact
     1861 gcctgcaaat gttacttgcc taacttgcct gcccaccatg tttttaattc tcacagcggt
     1921 taggtttgca tcatccacat tcagcatttt tatcacaaac aaacctgtag cagggttggg
     1981 tattatttca gcatcgggtt ttgttctatt gtttttgaca aaggtaagct caatgccttt
     2041 gtttggagaa attttactgc tgctgctatc gtttgcagga ttttgagcca cccgcgcaaa
     2101 accggtataa tccggacatt cggcaaaggt ttcaccggta taaatatgta cttcgcttgt
     2161 gctgcttgca tgaaattctg tatttatatt tatttccttt cttgctgtaa tattacttac
     2221 tccatcaatt attgtattgc taacggtaac actgttccag ccttttatat tttttaccat
     2281 gctcacaggt atgttttccc cgtctactaa aaaggcagcg ttagctgcct ttatttctgt
     2341 ttctgttttc attcaaacac tatcttaccg ttatagcttt tctttttact gcttaatgtt
     2401 acattataca ctccttttgc ctctgcctca aggtttactt ttattttacc ctcattcatc
     2461 tccaatgttg tttgctgtat caatttccct gtgttgtcat aaatggataa aaccaaattt
     2521 ttctcattca caaaatcatc aggcacggtt atgttgcatt tgcctgcatt ggggttggcg
     2581 tatataatta attgattgtt tggattagtt tctctgccgc ctatgcctgt tatggcatca
     2641 atcttagcta aaaataaatc attagataaa taactagtaa atgtattccc tcctatatta
     2701 acagtgtttt taaaaaaacc tccacaaatt acattattgt tactatcaat cgaaatgctt
     2761 tgacaacttg cctcaccaaa gtttctaacg cccatgcaat tcccattttg atcatatctg
     2821 gctaaaaaca tatcactggc gtttgaggta aataagttaa atgcaccgaa tgatgcaaaa
     2881 ccagaaaata gccctgctat ataactattt ccatctaaat ctgataatat ttgattgcca
     2941 gattgtaaat ttccggtaac aaataattgc ttattccaaa taaggttgcc gttcacatca
     3001 aatttggcaa taactatatc ttttcctgta ttatatagat tcccatttcc aaaatcaata
     3061 gaatcttgaa atccaccaat tatataaata ttattaaaat tgtcaataga aagtgattgt
     3121 atgtaatcta agccagcatt accaaatgtt ttaatccact ttatgcctcc attgctatct
     3181 gctcttgcaa taaaagcatc ataattacct tggggtatta aaagtgcagt atcaatttga
     3241 aatgggattg atcggaaagc accgtaaaga aaaatatctg aaccaataaa attaaggaaa
     3301 acagcatttg tattgcctaa aaccggtgca gtgaatttat tttgtgcata atagcaaata
     3361 ccgttaggat catacttggc gatagttccc ccaggcccca catgaaaacc atcaaagtaa
     3421 acagagtcgg ctgtttgtgt taataaatat atgttccctg aagaatctac ataaatgtta
     3481 ggctctcgtt taatcccatt tcccgtacat ttttttgccc aaataaaatt tccatttaaa
     3541 tccattcttg caataaaatt atcaacatta gatgtagagt aagaatttag gatgatgcca
     3601 ttcccaaaat caattgtgtt aataaaggta cctgaaagat agatgcaatt attaacagga
     3661 tcaaatacag cattggcatt ttcatgataa gtaatattag tattagagct acctatttgt
     3721 ttagaccaaa tttctccccc ccccccatca aatttagaaa taaatatatc gtcaaaacca
     3781 ttactaaaaa gtgtatcgtt aggcaagtat aaacaaccgt agaatttacc aataacataa
     3841 atattactgc catctgaaat gacactgttt atcttttcgt tgtaagaacc acaatcacta
     3901 ccaatatgct ttgcccactg ccaattttgt gctgatgtta acttagtgct aacaagtaaa
     3961 attaaaaata taaaatactt tttcatcttg ttttattttt agttgatgat tatttttttt
     4021 acaattttgt tattatcaat tatagcctca acaaaataaa ttcctttagg aaagtcatgt
     4081 aaatttaaag aaataaaatc ttttgttgag ctaaatttct gtagttcttt gccaagaatt
     4141 gattttattg aaacggtggc aacattattt tcattacaat aaatttccaa agaaaatgta
     4201 ccgttattag gatttggaat tatagagaaa tcgcaattct tattctttga aaatcgtatc
     4261 tcaatttcat tatgattagt cagttgattt tcttcatgaa ctgaattatt tgatgcaaaa
     4321 cgagaataag ttgatgtggc atcgcaatca atgaatgcat ctgacggaaa aatatgcacc
     4381 tcgcttgtag cgcttgcatg aaactcagta ttaatgttta tctcttcccg tgctgtaatg
     4441 tcactgatgc cgtcaatggt ggtattgctt actgttatgc tgttccagcc ttttatattt
     4501 ttttgagtgc ttacaggaat gttttcattg tcaaaaacac catcatactg aaagttaatc
     4561 ttgtcaagcg taatgttgcc aagtatagcg ggttgaattt gagcatctgt ccatccatcg
     4621 ggattaatag atccccatgc ttcccctctt tcaaactttt cagccacagg cgcagaacct
     4681 cttatatata taattctatc atcaccaata tttatttcat tatatggtat ggcttcaaaa
     4741 tcaccgttat catctgcata ataataaaac catgtatgaa catattcatc atcattagga
     4801 ttacttggag tttggttatc gtccaaatac aaccaactca acgcctgcac cacaccgcct
     4861 ttaatggggt ttgcgttagc atctttaagt gttcctgtaa cggcattgtg gtgtgtggtg
     4921 ttaaacaagc cgcagttgta ggggtcaagg taattagccg ttggcgcagc acagggcgca
     4981 ggtgcaggtg tgccattgcc ttcatcaaat gctattgtga aattaacagc aggtttatcg
     5041 gcagttgagt aataatttac agggtcggca tattgcagca agccccagta gttgccatgt
     5101 atttccttcg gtgttgaagg tatgcctcca caagtattgc aatacgtgca ggtcgagcag
     5161 atagcagaag gaatacagta ccagtgcaca tccatgaatt cccaccaact gaaacccgaa
     5221 ccgccacagt cgcgcacagc aggcaaaatg cttgctatgt aattatactg gtcggtatat
     5281 tcaccgtcag cagctcccgg atagctgtta atacacgcat cgggggaggt gctgagccct
     5341 gtttcgccta taatccaagg gtgttgcagc gcgctgctgc accatgaaat taaatcaagc
     5401 acccgtgctg tagctttagc ctggtcttta ttttcataat caggatatac agggtacagg
     5461 tgcatggaaa taaaatcaac tttcatcatg ctgccgtccc agtccatcac atctcccata
     5521 tccgttccgc caattgttac caaatggttc gggtcagatg ttttaatggc atcgtaccac
     5581 cagcctgtgt attcgcatac tttctgtttg ctttgagtta aattatggtc ataaacattc
     5641 cagtgcggct cattatacaa atcataagca agtagagcgg gtttgttttg aagatatgag
     5701 gcaagaacat gcaaatattg tgcatagtcc aatgcatcaa tgtctgacgt gcctctgtca
     5761 ttttctccta ctgatgagcc gtcagagcaa agaaatataa ctttaatatt attgttttca
     5821 gcaatgtcga gcacatgagc aactaaatta aaaaaagttt gattgcttgc gctgttgtaa
     5881 ggcgaattaa aatcaaaagt atattgcatg ttttcgttac acatatcggc aaagtaattt
     5941 gatttgtaag taaagccaag tgaaccgttt ttccggtgag gagataaaag caaccggatg
     6001 gaattaaatc ccatttgatg taatttggca aaatcttgag taagactgtt ttcgcaatcg
     6061 gcatacgtat aacctccttc taaggcacag cccattgtgc cgtaagcaga agaccttgta
     6121 actttgaatg ttgtagggtt agaagtttgg tcatgaacaa tatcaacatg ataattcata
     6181 accattggaa agaaaggtaa accagcagta tcataaaatt gccgtccttt tagcgttaca
     6241 tatcctgtgc cttgcccaat actaatatag caacaggcta ttgataacac cataataaat
     6301 aagcaaatct ttttcatggc ttatttattt tttagttcta taaactaact ccttcaattc
     6361 atccacttgt ttttgcaaac taataatgta caaagtttgc tcttcaactt ttttcagcaa
     6421 cttggtctgc atatcaccca attcaaaacc atcattactt tcaatttcct ttgcactcgg
     6481 tatttcaggt aaatgtttgt tcaactgcaa atattcctca agttcataaa tgctcatcaa
     6541 cttataatcc tttgcaaaaa cataatctgg gaagggcaag gctgttactt tcacatcgcg
     6601 tgtcattatg ccgccctcta catataattt gtagggcgaa atggagcaat cgctgcaatt
     6661 attaaaccca acaaagactt tgcctatatt ataaattcca ttggcatcgg tagcccacaa
     6721 gccggcattg cctgaggcaa tgttacttgc tactaaattt ccgttagcat ccagttcaat
     6781 taattttccg ttattgcccg ataaacttct tacccgcaaa tcaccattta catctaattt
     6841 agcagtaggc acatccgtat caattccggt attattgctg gttccatcta taaaaaatgc
     6901 agtacgctgt atagccagcc catgattcca aataaaatat gactgattat taatgcttcc
     6961 taaataacat cccgttgccc acttttgtac accctgataa ttaaatttta tttcactggt
     7021 ataatattgg tttgaaacct gattaattgc aataccgcca tattggtcac tatgagtaat
     7081 ttccaaagcc tgtgaaggac ttgtacttcc tatgcctact cttcctgttg cagtagctaa
     7141 aaaagtatta ccacctgttt ctacatcgcc tttagttgtt ccggtattta ggtaaatgtt
     7201 tttaccactg taatagttaa tcagtacatt atccgttgag ttaatacttg cattagcgcc
     7261 atcgtgctcc acatctaaat attttgtgtg gtcattaaaa ttaaaaaaac gtgtgccccc
     7321 ttttacacct aagtttttct gtgggtcatc cgtccctatg cctacctgat tccagcaagt
     7381 gtaagcaata ttgggcaata tgttagtttt aatctgccat gcaaatgtat atgggtctgt
     7441 acaggattga gtcggattta caactatagg tcccggtatt aatttcccat tggcatccga
     7501 ggttaaaata cgatttcctg ttcctgtaaa agagttgatt tttatatcac cattacccaa
     7561 caacctcaat cgttcactgt tattggtttt aaaaaccaaa tctttattat cgttagtgcc
     7621 aataaaatta tcagtagcgt tgctgcccca gtttcccgtc atagtccagt cgtcacgctg
     7681 cggctctttg aggttgatgc tgctgctggc tttgcagccg ttgaggtcgg ttactgttag
     7741 tgtattttct cgcccgcaaa ggttggatgg tgcaaggatg ttttgctgtc cgggctccca
     7801 atgataagtc aagggcaaca cacctccgtt gatgagcgtt gttacactgc cattgcaggc
     7861 accataggtg cttacattaa aatcgttggc atagtagtag ggtgtcattt ctgcaatggc
     7921 cagctgctcg ggctgcgtga gtgttatctg cagcgtggtg tcgccaccgg cagcatcgcg
     7981 cacccgcacg gcatagtagc cggcagctaa agaactcact gtggctaagg tgtcgccatt
     8041 gctccaggtg tagttgtatg gagcagtacc attgctgatg ttgacggcaa gtgttccgtc
     8101 ttgcatgcca tagcaactaa tgttgtaacc attgtaactc gatgcttgta gtgtggcctg
     8161 catttgtgca tgtgcaaaag agctaacaaa aagtgttagc gcaacaaggg caactgttgt
     8221 tgcggggggg gggggggaaa aaactttttc atttggcaga ggattttaaa attcgaatgt
     8281 aaatataagg attgttaagt aatgtcaaat aacaccactc cattagcgat gacggctatt
     8341 tcacatttta tacctattgg cagtaagcgt tggctactct tattttaact aaaagcttct
     8401 ttatgtataa aagcctgaaa ctcgttacgc agttctttaa aatatctttc aaaagaacca
     8461 agtttttcac gttcactctg atgtgcagct gcaagatttg ttaccggatt tgaagagtgt
     8521 gcttcaattt tattttcatg ctgtttaaaa tcatgtcgca tgatatccag aat
//
LOCUS       DAOQNP010000098         8157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3265_length_8157_cov_6.845717, whole genome shotgun sequence.
ACCESSION   DAOQNP010000098 DAOQNP010000000
VERSION     DAOQNP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_8157_cov_6.845717"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKY09_11185"
     CDS             complement(<1..595)
                     /locus_tag="PKY09_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNB13461.1"
                     /translation="MTTFAIDLFMQVLQKTSQYLSLVKFSHTVFALPFAAIGFSLAIS
                     QNGLPVFWPLAIKVLVCMVTARNAAMAFNRWADRNIDALNPRTALREIPAGIIKANRA
                     LWFVIINALLFTVTAYLINNLCFYLSPVALFVVLGYSFTKRFTALCHLVLGAGLALAP
                     VGAYLAVTGRFDIVPILIGVAVLCWVSGFDIIYALQDE"
     gene            643..1215
                     /locus_tag="PKY09_11190"
     CDS             643..1215
                     /locus_tag="PKY09_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HNB13462.1"
                     /translation="MNFLAHLYLSGNNEPLMIGNFIADHLKGNAWESLPSEIIAGVKL
                     HRFIDHFTDTHAVVEQSKALLRPYFHKYTPVISDIFYDHFLAAEWNNYSDLPIDEYAK
                     NIYQLLHKNYQFLPERTKEMLVYMEHDNWLKNYATLNGIHRALSGMGRRARFDNNMHQ
                     ASEFLYDNYDAFKKHFEVFFPELQKAVKSF"
     gene            1374..2753
                     /locus_tag="PKY09_11195"
     CDS             1374..2753
                     /locus_tag="PKY09_11195"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNB13463.1"
                     /translation="MDFRFLTIESQQQGVIAFIAVVLFQMLFVVVQWILHRRVDFSYY
                     LFYLFTVVLYAVSLYRHILNIGDIFPLGEWLLEINIYPLPLLSVFFYFRFQRSFLELS
                     IHNPELNLVVYKLERFILTYCIVAPLLVLAGVNGTILYILFVTVACVTIITSLFIVIK
                     FLKKKIPLEQFSIIGAIFIISGSLVTVVLGIQEKQLTPLIVNPHLPLMVCALAELFTF
                     TTGLTYKSYLIEMAKVKSEKDLLVAEQKRQKMEIQYLKMRNDVTKNLHDKLGSSLSLA
                     KLLIERIDNSNRNGDSSFVSNSVRALDDAIKSLHQIINNLKSDTVEPYDCVNSINQMI
                     AMLKPINNIVIHFNHNLDNHKISVYLHDQLSHIILELINNSLKHAKATNISIDLMKHN
                     EMVKLKYVDNGKGLDLLKCNYGNGLRNIAERVSGCMGTLNYLSEEGKGFVAKIEIENK
                     IQLTKLETA"
     gene            2765..3391
                     /locus_tag="PKY09_11200"
     CDS             2765..3391
                     /locus_tag="PKY09_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB13464.1"
                     /translation="MKMKLIIADDHLLFAHGLKELLAKSFPIEMITVVNNGKEAIEAC
                     LKNKADCVIMDVDMPVMDGIYACRELKRMSSNIKVIILTMHSDLISLSTAWSAGADAY
                     LLKGNDVDEVLNAFDAVMKNKRYVCDALSHLIDNRDKPNQKIEAPISTRETDVLKLIC
                     QGLTNEVIAKIYSISCRTVDTHRNNMLIKLKLPNTAALVRFAIENKLV"
     gene            3479..3670
                     /locus_tag="PKY09_11205"
     CDS             3479..3670
                     /locus_tag="PKY09_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNB13465.1"
                     /translation="MYLTKREKEVINLLGRGLSDKSIALRLKISYNTVRTHRQNLRAK
                     FKVSTTVAMIRMASEMSMI"
     gene            3779..4339
                     /locus_tag="PKY09_11210"
     CDS             3779..4339
                     /locus_tag="PKY09_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13466.1"
                     /translation="MKKLMIIVCAVAITALMGCGKDGETGPQGEQGPAGANGTNGVDG
                     TNGTNGTNGNANVKLFMFGPTTFTSTDDNETYNYPSSVKTNMLDSSLVLYYHKTSSSS
                     AWYATPGLGNGANYQTRAYTFSSTRNFIIEIADADGSAYSGASRTFTSIKAIVVPAST
                     YTGSRNSGVNFNDYEATMKHFGLPLD"
     gene            complement(4396..4695)
                     /locus_tag="PKY09_11215"
     CDS             complement(4396..4695)
                     /locus_tag="PKY09_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13467.1"
                     /translation="MRCRKKATTIISNRTIYSKILLKLNWQYFCINTYSISIPYGCLV
                     SYSKLIGDYKLNLKIQELPIRYRERTCGSTNISRFKHGVILLRMCFFAARKIKFA"
     gene            4857..>8157
                     /locus_tag="PKY09_11220"
     CDS             4857..>8157
                     /locus_tag="PKY09_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13468.1"
                     /translation="MKKAFLLSLFLFAILHAYPLSIVISDQYSNSLTSGNACSGTVLH
                     VISCNGCVSFQWQKNGTNIASATGGSYTVTQSGTYSCIATDANGVTATSNLMQINIVA
                     SPPTPKLYTTISGILDDCYDIILLDTSCWFIGALYVLEPYDNSWYADYPNPNGITGVM
                     SGFYRISLYSVCGNTDNNVTIRNKGIFGLYKNRVASKTGKTNLCGSDSIELCVSNDAV
                     TIPTGCSEVQWDSYSWTRDGIPISTSQRIYAKLPGTYKCLISNSCDNGQPQLIDSLVI
                     TNFTASASITASGPTNICQGNSVTLSVTPTAGATYSWYKDSVLIPGIFGTSYTVTTAG
                     TYKVEVKYFGCKFMSTDVVVTTFPVPYTPILSSTSPTILCSGGTASLDASPSGSGYSY
                     TWYADGVVVPGASSSTYSPAVQGNYTVKITDAQGCESTSSKMSVVYGTHSASISSSNA
                     NPICELKSFTMQSNAVGSDITKYQWKLNGNNIVGANGTTYIQNVTLASAGNYTVDITN
                     ACGVFTSPVYALNVNAKPNDPVVTYTGNNFFCTTGTVSLNSITPAGLTYEWLKNGSVI
                     SGGTSTTYSATTGGAYSIRHTNASGCTATSNVVDVYTTGFAPVITPSGFAIGCAPFVL
                     SVTPSSGSYTYQWVNSSNYYGPYSTIAGANSSSYAAPVTNYPNNKFYKCIITGPCGTA
                     TSNIVEVNIKNSVPTSKILPTSSSGGYIKCDGQVVTLYDNYAPGTIKGIYYQWTKNGV
                     NVNGATSRVYFPTTSGVYRCAVTNGCGTKLSTDSAVITFNTNPAANIIASGSTNLCNG
                     ASVTLNANTGTGLTYQWTTNGQEITGAINASYTTSIGGTYRCIVTNNNGCSATSNFIN
                     VFTSGTTPTSSITPSGTVNICIGATQTLSANTGNGLSYQWTLNGNNITGANLPTYNAN
                     VPGSYAVIVTNSNLCSATSAATIVNIGTITGQISPAGTVNFCAGLNTTLTATTGTGYT
                     YQWYRNNVLLSSTNSSINVGSGGTYKCVITSGSCSTTTNSVVANQINNPTPAITHVTP
                     TTFCAPGSVVLMANNFAGVQYQWQKNSVDILGETSQIYTATSNGSYRVIETANGCSKS
                     KTIAVNAASSVS"
BASE COUNT         2606 a         1498 c         1551 g         2502 t
ORIGIN      
        1 gctcatcctg caaagcataa ataatatcga atccactaac ccagcaaaga acagctacgc
       61 caattaaaat gggaacaatg tcaaacctgc ctgtaacggc cagataagca ccaacaggag
      121 ccaatgcaag tccagcacct aaaactaaat gacaaagtgc cgtaaagcgt ttggtaaagc
      181 tgtagccaag tacaacaaac aatgcaacag gcgacagata aaaacaaagg ttattaatta
      241 agtatgcagt cactgtaaaa agtaaagcat taataattac aaaccacaat gcacggttag
      301 ctttaatgat gccggcagga atttcgcgta aggcagtacg tgggtttaag gcatcaatgt
      361 ttctgtcggc ccaacggtta aatgccattg cagcattacg tgcagtaacc atgcagacta
      421 aaacttttat tgccaacggc caaaagacag gcagtccatt ttgagaaata gccaacgaaa
      481 agccaatagc agcaaaggga agtgcaaaca ccgtatggct gaactttaca agtgataaat
      541 attgtgatgt tttttgtaag acctgcataa ataaatcaat tgcaaaggta gtcattcctg
      601 atggtttgcc ttttcaaaga agctatttgc tacattagcc caatgaattt tctggcacat
      661 ctgtatcttt ccggtaacaa cgaacctttg atgataggca attttatagc cgaccacctg
      721 aagggcaatg catgggaaag tctgccgtca gagattattg caggtgttaa actacaccgt
      781 tttattgacc attttacaga tacacatgct gttgttgaac aaagtaaagc attgctgcgt
      841 ccttattttc ataagtacac acctgtaatc agtgatatat tttatgatca ttttctggcg
      901 gcagaatgga ataattattc agacttgccc atagatgaat atgcaaagaa tatctatcag
      961 ctcttacata agaattatca gtttttgcct gaacgtacca aagaaatgtt ggtttacatg
     1021 gagcatgaca actggttgaa aaactacgct actttaaacg gtattcatag agcgttgagt
     1081 ggtatgggca ggcgtgccag gttcgacaat aacatgcatc aggcatctga gtttttatac
     1141 gataactatg acgcatttaa aaagcatttt gaggtgtttt ttccggagtt acagaaagca
     1201 gtaaaatcct tctaataaac agtttaattt gtcgcccgac actggtttat ggtgcagaat
     1261 tttggggttt gcattatgta taacgaaaat ggtgtttgat acataatttt tttatttttt
     1321 cttacaatct gattatattt ggtgcaagaa aaaatccatt tactccaatt accatggact
     1381 ttagattctt aactatagaa agtcagcagc agggagtaat agcttttata gctgtggtgc
     1441 tgtttcagat gctctttgtt gttgtacagt ggatactcca cagacgtgta gatttttcct
     1501 attacctatt ttatttgttt acagttgtgt tgtatgctgt aagtttatac agacacattt
     1561 taaacatagg tgatatattt cccttgggtg aatggctttt ggaaatcaac atctatcctc
     1621 taccattatt gtctgttttc ttttacttcc gttttcaacg atcgtttttg gaactttcaa
     1681 ttcataaccc tgagctaaat cttgtagttt ataagttgga acgttttatc ctgacatact
     1741 gcatagtagc tcctttactt gttttggctg gagttaatgg aactatctta tacattcttt
     1801 ttgtgactgt ggcatgtgta actatcataa cctccttgtt tatcgtaatt aagtttttaa
     1861 aaaagaaaat cccacttgag caattttcaa ttattggagc gatttttatc atcagtggtt
     1921 cattggtgac tgttgtattg ggaatacagg agaagcaatt gacaccctta atcgttaatc
     1981 cacatttgcc gttaatggtt tgtgccttgg cagagttgtt tacatttact acaggactta
     2041 cttataagtc gtacctgatt gaaatggcta aagtgaaatc cgaaaaggat ttattggtag
     2101 cagaacaaaa gaggcaaaaa atggaaatac aatacctgaa gatgcgtaat gatgttacca
     2161 aaaatctgca cgataaactt ggttcaagtt tgtcattggc caaattactg atagagcgga
     2221 tagataattc gaatagaaat ggtgattcaa gttttgttag taattctgta agagcactgg
     2281 atgatgcaat aaaatcactc catcaaatta ttaataacct aaaaagtgat actgtagaac
     2341 cttatgattg tgtgaattca ataaatcaaa tgattgccat gttaaaacca attaacaata
     2401 ttgttattca ctttaatcat aaccttgata accataaaat ttcagtttac ttgcatgatc
     2461 aactctcgca tattatcctt gaactgatta ataattcgtt aaagcatgcc aaagccacta
     2521 atattagcat tgatttgatg aagcataacg aaatggtcaa gcttaaatat gttgataatg
     2581 gcaaagggct ggacttgtta aagtgcaact atggcaatgg cttgaggaat atagccgaaa
     2641 gagtaagtgg atgtatggga acattgaatt atttgtcaga agagggaaaa ggctttgttg
     2701 ccaagattga aattgaaaac aaaatccaat tgacaaaatt agaaaccgca taaatttaaa
     2761 aaatatgaaa atgaaactaa ttattgctga cgatcatctg ctgtttgctc acggcttgaa
     2821 agagctttta gctaaatcat ttccaataga aatgataact gtcgttaaca atggtaagga
     2881 ggccattgaa gcctgtctaa agaataaggc agattgcgtg ataatggatg ttgatatgcc
     2941 ggtgatggat ggcatttatg cctgtcgcga attaaaaaga atgagctcga acattaaggt
     3001 tataatatta accatgcata gcgacttgat atcgttaagt actgcctgga gtgccggtgc
     3061 cgatgcctat ctgctaaaag gtaatgatgt ggatgaagta ttgaatgcat ttgatgcagt
     3121 gatgaaaaac aaacgatatg tatgtgatgc tttaagtcat ctaattgata acagagataa
     3181 acccaatcag aaaatagaag caccaatctc gacaagagag acagatgttc taaaattaat
     3241 ttgtcagggg ttaaccaatg aagttattgc aaaaatatat tccatatcct gccgaacagt
     3301 ggacacccac agaaacaata tgcttattaa gctcaaactg ccaaacactg cggctttggt
     3361 gcgttttgca attgaaaata aattagttta gacggcactt ataattcaaa tacgcctttt
     3421 cacgtattga tgtaacaaat acttttttaa tactttgcac tgatattcta cagtggatat
     3481 gtatttaact aagcgtgaga aagaggttat taatttatta ggtcgcggcc tttcagacaa
     3541 atctatagcc ttaagactta aaattagcta taacaccgtg cgcacccacc gccaaaacct
     3601 gcgtgctaag tttaaggttt caactactgt agccatgata agaatggcaa gtgagatgag
     3661 catgatataa gaaaatagta aaatacgtct tttaacgtat tggttgtaag gaagccaaat
     3721 ttcattttta cagcattaaa aaaacaaatt aatatctaac aattaaaaat taaacaaaat
     3781 gaaaaaacta atgattattg tatgtgctgt tgcaataaca gcattaatgg gttgcggaaa
     3841 agatggtgaa accggcccgc aaggtgaaca aggccctgca ggagctaatg gaacaaatgg
     3901 tgttgatggc accaatggca caaacggtac taacggaaat gccaatgtga aactgtttat
     3961 gtttggacca actacgttta cttctactga tgataatgaa acttataact acccatcatc
     4021 agtaaaaacg aatatgcttg attcaagttt ggttttatat taccataaaa cttcttcttc
     4081 atcagcatgg tatgcaactc ctggtttggg aaatggagca aattatcaaa ctagggcgta
     4141 taccttttca tctacaagaa attttatcat agaaattgct gatgctgatg gttctgctta
     4201 cagtggtgca tcacgaacat ttacaagtat taaagctatt gtagttccag catcaacgta
     4261 cactggaagc cgaaattccg gagtaaactt taacgattac gaggccacta tgaaacattt
     4321 tggcctgcct ttagattaaa cataacaacc taaaatttac acgatgggca cagacaaata
     4381 agtttgtgcc tttttttatg caaacttaat cttccttgca gcaaaaaaac acatgcgaag
     4441 taaaataaca ccatgtttaa aacgcgaaat gttggtgctg ccacaagtgc gttcgcggta
     4501 gcggatgggt agttcttgta ttttaagatt gagtttgtag tcgccaatta acttgctgta
     4561 actgaccagg cagccgtacg gtattgaaat agagtaggtg ttgatgcaaa agtattgcca
     4621 gtttagtttt aataatattt tggaatagat tgttctgttt gatatgatag tagttgcttt
     4681 ttttctgcac ctcaaatgtg atttgagaca gggacacact ttttatctta ttcgttagtt
     4741 cggggcgcaa ttgtttggaa atagaatggc caaagaggtt atctgtcttt tttttaaaaa
     4801 atgttttgtt ttttgaaaaa ttatgttagt ttcgcttaca aataaattat actaatatga
     4861 aaaaagcatt tcttctctct ttgtttctgt tcgctattct ccatgcctat cctttgagta
     4921 ttgtaattag tgatcaatat tccaattcat taacttcggg aaacgcctgt agcggaaccg
     4981 tactgcatgt aatctcttgc aatggatgcg tatcttttca atggcaaaaa aatgggacta
     5041 acattgcttc agccaccgga ggcagctata cggtgaccca atcaggaaca tattcatgca
     5101 ttgctaccga tgctaatggt gtaacagcaa ccagcaattt aatgcaaatt aatattgttg
     5161 catcaccacc aacccctaaa ttatatacca ccatttctgg aattttggat gactgctatg
     5221 atattatatt attagatact tcgtgctggt ttattggtgc attatatgtt ttagagcctt
     5281 atgacaattc atggtatgct gactacccca atcctaatgg cattacggga gtaatgagtg
     5341 ggttttacag aattagttta tattctgtct gtggaaatac cgacaacaac gtgactattc
     5401 gaaacaaagg tattttcggt ctttataaga acagagtagc atctaaaaca ggtaaaacca
     5461 atttgtgtgg gtctgattca atagaactat gtgtttccaa tgatgcagtt actattccaa
     5521 cggggtgtag cgaagttcaa tgggattcct atagctggac aagagatggg attcctatta
     5581 gtacgtctca aagaatttat gccaaattac cgggaactta taagtgttta atctccaact
     5641 catgtgacaa tgggcaacca cagcttattg attcattagt gataaccaat tttacagcaa
     5701 gtgcgtctat tacagcatcg ggacctacta acatttgtca aggaaacagt gttacattat
     5761 ctgtcactcc tactgccgga gctacttatt cttggtataa agattctgtt ctaatacctg
     5821 gtatatttgg tacttcttat acagtcacta ctgctggaac ttataaagtc gaagtaaaat
     5881 atttcggatg taaatttatg tccacagatg tggttgtaac tacttttcct gttccgtaca
     5941 caccaatttt atcatcaact tctccaacca ttctttgcag tgggggtact gcctctttag
     6001 atgcaagtcc atctggcagc ggctattctt atacctggta tgcagatgga gtagttgtac
     6061 caggagcctc ttcatcaact tacagccctg ctgtgcaagg aaactacact gtaaaaatta
     6121 ctgatgcaca gggttgtgag agtacatcat caaaaatgtc agttgtttat ggtacccata
     6181 gtgccagcat ttcttcgtct aatgccaatc ccatctgtga actcaagtct tttaccatgc
     6241 aatcaaatgc agttggtagt gacataacaa aatatcaatg gaaactaaat ggtaacaaca
     6301 tagttggtgc taatggcaca acttatatac agaatgtcac acttgcttct gccggcaact
     6361 atactgttga cattaccaat gcatgtggcg tttttacttc gccagtttac gcattgaatg
     6421 tcaatgccaa accgaatgat cctgtagtta cctatacagg aaataatttt ttctgtacca
     6481 caggaactgt atcacttaac tccattacac ctgcaggatt aacttatgaa tggttaaaaa
     6541 atggtagtgt gatatcaggg ggaacaagta caacttacag cgcaacgaca ggaggagcct
     6601 actcaataag gcataccaat gccagtggat gtactgccac ttctaacgtt gttgatgttt
     6661 acacaacagg gtttgctccc gttattactc ccagcggttt tgcaataggt tgtgctcctt
     6721 ttgttttatc agttacccct tcttcaggat cttatactta ccaatgggta aatagttcta
     6781 attattatgg accctattct actatagccg gtgcaaattc aagctcctat gcagcacctg
     6841 taacaaatta tcctaacaac aagttttata agtgtataat aacaggcccc tgtggtacag
     6901 ctacttcaaa tattgttgaa gtaaatatta aaaactctgt acccacatcc aaaattttgc
     6961 ccacttcgag ttcaggcgga tacattaagt gcgatggaca agttgtaact ctttatgata
     7021 actatgcgcc aggtacaatt aaaggtattt attatcagtg gacaaaaaat ggagttaacg
     7081 taaatggcgc cacatccaga gtttattttc caactacaag tggtgtttac agatgtgcag
     7141 taaccaatgg atgtggcacc aaattaagca ctgattctgc tgttattact tttaatacta
     7201 atccagcagc aaatatcatt gcaagtggct ccaccaattt atgtaatggc gcaagtgtaa
     7261 cattaaacgc aaatacagga acagggttaa catatcagtg gacaacaaac ggacaagaaa
     7321 ttacaggagc tatcaatgca tcttatacta cctctattgg tggaacctat cgttgcattg
     7381 ttaccaataa caacggatgc agtgctactt ctaatttcat aaacgtattc acatcaggta
     7441 ccacacccac ttcaagcatt acaccatcag gtactgttaa tatttgtata ggtgcaacac
     7501 aaacacttag tgccaacacc ggcaacggac tctcttatca gtggacattg aatgggaata
     7561 acattacagg agcaaatctc ccaacttaca atgccaacgt acccggcagc tacgcagtaa
     7621 ttgtgaccaa cagcaatctt tgtagtgcaa cctctgcggc aactattgtc aacattggaa
     7681 caataacagg acaaatttct cctgcgggca ctgttaactt ctgtgctggt ttgaatacta
     7741 cgcttaccgc tactacagga acaggttaca cctatcaatg gtacagaaac aatgtgctgc
     7801 tttcaagtac aaattcttct attaatgttg gttcaggcgg tacttataaa tgtgttatca
     7861 cttcaggttc ttgctcaacc accaccaaca gtgtagttgc taatcaaatc aacaacccta
     7921 ctccggctat tacacatgtc acgcctacaa cattctgcgc accgggttct gttgtactaa
     7981 tggcaaataa ttttgccggt gtacaatatc agtggcagaa aaacagtgtg gatattcttg
     8041 gagaaacttc tcagatttat actgcaacat ctaatgggtc gtacagagtt attgaaactg
     8101 ctaatggttg ctctaaatca aaaactattg ctgtaaatgc tgcatcttct gtttctg
//
LOCUS       DAOQNP010000099         8095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3296_length_8095_cov_11.127861, whole genome shotgun sequence.
ACCESSION   DAOQNP010000099 DAOQNP010000000
VERSION     DAOQNP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_8095_cov_11.127861"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2012
                     /gene="uvrA"
                     /locus_tag="PKY09_11225"
     CDS             <1..2012
                     /gene="uvrA"
                     /locus_tag="PKY09_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNB13469.1"
                     /translation="GACSRCNGLGTIPEADLSSIIPDNKKSIRKGGILPLGEYKNNWI
                     FGELEYIGRRFGFNLNTPIHDIPHEALNIILYGTDDVSLNATPGSNHGITFEGIVNFI
                     VNQMEENATYSIKKWALNFMQTITCPECEGTRLKKESLAFKLNNKNIAELSLMDLSEL
                     QQWFQQLPASLNEKQNIIAIEVLKEIVKRLGFLLDVGLDYITLNRSTRSLSGGESQRI
                     RLATQIGSQLVGVLYILDEPSIGLHQRDNERLIKALKDLRDIGNTVIVVEHDRETIEA
                     ADFVVDLGPGAGINGGDIVAIGKPEELKFQQSLTANYMSGGIKMPSSKNRKGNGKKLT
                     ITGAKGNNLKNVTAHFPLGTLICVTGVSGSGKSTLVNETIYPALNKHFYNSSQKPLPY
                     EKINGLSHIDKVIEIDQSSIGRTPRSNPATYTGVFTDIRNLFAQLPEAKIRGYAPGVF
                     SFNVSSGRCDTCLGAGLKIIQMNFLPDVHVHCETCNGKRYKREILEVRYRGKSISDVL
                     NLSINEAVDFFSNIPAILHKIKTLQDVGLGYITLGQSSTTLSGGEAQRVKLASELSKK
                     DTGNTFYILDEPTTGLHFEDVRILLLVLQQLVDKGNTVVVIEHNMDIIKAADYILDLG
                     PEGGKKGGNIVAEGTPLQISKNTNSITGTFLKKEMEWHNKVSAEV"
     gene            complement(2030..2893)
                     /gene="prmC"
                     /locus_tag="PKY09_11230"
     CDS             complement(2030..2893)
                     /gene="prmC"
                     /locus_tag="PKY09_11230"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNB13470.1"
                     /translation="MMRLISFATMTLKEIISSFQAEVDLIYDKSESVAITRLVIEHVF
                     GSDYQTSNTVIVNVSQETLIHQYLERLRNYEPIQYILGQAWFCNHIYKVNSSVLIPRP
                     ETEELILLMKQNLKSEHVGSIIEIGTGSGCIAIELSLLFPKALVKATDISAEAINVAI
                     KNASDLGASVTFVNESVFDSGTETLQSKVDIIVSNPPYIAQHEAKEMHPNVLKWEPHT
                     ALFVKEHPLEFYITIAALATKILNENGYVFLELNPTYANQIAQCFKDKGFNQVEIIKD
                     ISGKERFLKVQ"
     gene            2923..3954
                     /gene="ribD"
                     /locus_tag="PKY09_11235"
     CDS             2923..3954
                     /gene="ribD"
                     /locus_tag="PKY09_11235"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNB13471.1"
                     /translation="MTFDETIMHRCLELAKMASGQTIPNPMVGSVLVFENKIIGEGYH
                     QRAGEAHAEVNAINSVENKSLIPLSTLYVSLEPCSHYGKTPPCCERIVQEGIKKVIVG
                     TLDTTTKVNGKGISYLQQNNVEVLTPMLNDACRFSNRRFFTYHEKHRPYIILKWAESA
                     DGFIAPENTQQQYSISNTFSKTLSHKWRTEEQAILVGTKTAIIDNPQLNSRLWEGNAP
                     IRVVIDKNLAIPTSHHLFDGSVKTILINQKKEISENNINYIKINFQENPATQILNVLY
                     QLNIQSVIIEGGTKTLQLFIQNNLWDEGRVFTAKKQLIRGIKAPEFSISPSEITTIAD
                     DTLKIFYNV"
     gene            3969..4811
                     /locus_tag="PKY09_11240"
     CDS             3969..4811
                     /locus_tag="PKY09_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13472.1"
                     /translation="MAVLFASLLFVILKLFARYKISNIHALTYNYFTAGICYTLYSKQ
                     TFSSIAGFVASYFPYYLLSGCLFIGVFYLTALSSQKNGVASTTIASKMSLVIPALLAV
                     YLYNETFSLLKTIGLLMALSAVYLSARGSNPESSESGWKLPLLVFAGAGLVDASIKYT
                     QHVFTDNAGSSGFLSLVFFSAAIIGSFVLLFKKNKSIGGNKFSNIIAGIILGFFNFIS
                     LYYMIAALQEFHEDSGKAFIIINSGVVLLNFLLSVILFQEKKNTQSRVGVTLAVAAII
                     ILSQ"
     gene            4812..5429
                     /locus_tag="PKY09_11245"
     CDS             4812..5429
                     /locus_tag="PKY09_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNB13473.1"
                     /translation="MFETNYKSIIGHSEGVFRDKGSRFLAFAYYVETENECKSVLERI
                     KKEHPKANHHCYAYRLGPYKNIFRWYDDREPSNTAGRPIYAVIQSNDLTNILIVVVRY
                     FGGTLLGVPGLIQAYRSAAEAAIEQNTIIEKEIEEEYELQMSFEEMAEVMKIVKQHKA
                     TFTQTSTADTLTLLVKTKKTFSDHFLKALTENYLLKEKIKIKALF"
     gene            complement(5432..6586)
                     /locus_tag="PKY09_11250"
     CDS             complement(5432..6586)
                     /locus_tag="PKY09_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNB13474.1"
                     /translation="MREIKLSMKGQETFFISCAPHVVPWLKQELDALGYKPFDENYTG
                     LFIKAAFERCMELNFQLTTASRVLWLVDKFTATDQKQLYNKLSRIRWSDYFSEKKYIT
                     ITSQTNHPSINNSMFLNQLCKDAVVDFFQSHYNIRPDSGPLRSEVVIHLHWQENKAWV
                     YLDTSGEQLNKHGYRQNPFKAPVQENLAAAIIKATGWDKKSAFINPMCGSGTFAIEAA
                     LMAGNKLPLLSRSNFGYMHLFDFNKKKFEMLRSELALKSNFDNMPAIIATDNNGYAIK
                     AIRENAERAGVDVFIEMNKCDFAETLIPEGNGVIVINPPYGERLGENMDLEALYKSIG
                     DFFKQKCKGKTGFIFTGDLTLAKKVGLRTSARIPFYNGTIECRLLKYDLY"
     gene            6713..7603
                     /locus_tag="PKY09_11255"
     CDS             6713..7603
                     /locus_tag="PKY09_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNB13475.1"
                     /translation="MTENKKPNNQELIAMISLLDDEDETVYESLLSHFLEIGKPAIPL
                     LEEKWSQSLDALLQTRIENIVHKIQFEAVKTELTNWKEHNQKNLLTGLIAIARYQYPD
                     LDEDSINVFFSEVRRRVWDDLQPNDTPLEQVRILNQVLFVEFGFNGNTANFHSPQNSF
                     INTVIESKRGNPILLSVIYAIVAQDLDIPVYGVNLPEHFVLCYQTLHKDKFAEYAFPE
                     DNILFFINAFSRGSVFGKDEIDLYLRKLKLPSDKKFITPCSNTDIIRRILHNLNFSFN
                     KSGLTEKSEEIEILLALLEK"
BASE COUNT         2678 a         1495 c         1401 g         2521 t
ORIGIN      
        1 atggtgcctg ttcacgttgc aatggacttg gcacaatacc tgaagctgat ttatcttcca
       61 tcattcctga caataaaaaa tcaatccgca aaggtggtat tttaccatta ggtgaatata
      121 aaaacaactg gatatttgga gagttggaat atataggcag gaggtttggc ttcaacctga
      181 acacaccaat tcatgatatt ccgcatgaag cactcaacat aatcctatat ggcacagatg
      241 atgtttcgct caatgcaact ccgggcagca atcatggaat tacgtttgaa ggtatagtaa
      301 actttattgt caatcagatg gaagagaatg caacctattc catcaaaaag tgggcgctga
      361 attttatgca gacaattacc tgccccgaat gtgaaggcac acgactaaaa aaagaatcgc
      421 ttgcatttaa actcaacaat aaaaatattg ctgaactttc tctgatggac cttagtgaac
      481 tgcagcaatg gtttcagcaa ctacctgctt cactgaatga gaaacaaaat atcattgcta
      541 tagaagtact gaaagaaata gtaaaacgtc ttggttttct gcttgatgtc gggcttgatt
      601 atataacctt aaaccgtagt acaagaagct tatccggtgg cgaatcgcaa cgtattcgtt
      661 tggctacaca aattggttcg cagttagtgg gcgtgttgta tattcttgat gaaccttcta
      721 taggactgca tcaacgcgat aatgaacgat tgataaaagc attaaaagac cttcgcgata
      781 ttggcaatac agtaattgtt gttgaacatg acagagaaac tattgaagct gctgattttg
      841 ttgttgacct tggcccgggt gccggcatca acggtggtga tattgtagca ataggtaagc
      901 ccgaggaatt aaaatttcaa caatcactca cagcaaatta tatgagtggc ggcattaaaa
      961 tgccttcttc aaaaaacaga aaaggaaatg gaaaaaaatt aaccatcaca ggtgctaaag
     1021 ggaacaatct taaaaacgtg actgcacact ttccactagg aacattaatt tgtgtgaccg
     1081 gtgtttcagg aagcggcaag tcaaccttag tcaatgaaac tatttatcct gcattgaaca
     1141 agcattttta taacagttcg caaaaacctt taccttatga aaagatcaat ggcctaagtc
     1201 atattgataa agttattgag attgaccaat cgtcaattgg tcggacacct cgcagtaatc
     1261 ctgcaacata cacaggagtt tttaccgaca taagaaactt atttgcgcaa ttacctgaag
     1321 caaaaatcag aggttatgct cccggagttt tttcattcaa tgttagcagt ggccgatgtg
     1381 atacctgtct gggtgcaggc ttgaaaatta tacagatgaa ctttttgccc gatgtgcatg
     1441 tacattgtga gacttgtaat ggcaaacgct acaaacgcga aattcttgaa gtccgctatc
     1501 gtggtaaatc catcagcgat gtactgaatc tgagtatcaa tgaagcagtt gattttttta
     1561 gtaacattcc tgccatttta cataaaatta aaaccttgca ggacgtaggt ttgggttaca
     1621 taacattagg acaatcatct actaccctat caggtggtga agcacaacga gtgaaattag
     1681 cttcagaact ttcaaaaaaa gatacaggca atacttttta tatacttgat gaaccaacaa
     1741 caggtctgca ttttgaagat gtaagaattt tactcttggt tttgcagcag ttagttgaca
     1801 aaggtaatac cgttgttgtg attgagcata acatggatat tattaaagca gcagattata
     1861 ttcttgacct tggtcctgaa ggcggaaaaa aaggtggaaa tattgttgca gagggcacac
     1921 cattacaaat cagtaaaaat accaacagca ttacaggaac ttttctgaaa aaagaaatgg
     1981 aatggcataa taaagttagt gcagaagtct gatattattt attactaaat cactgcactt
     2041 ttaaaaaacg ctccttgcct gaaatatcct ttattatttc aacttgatta aaacctttat
     2101 ctttaaaaca ctgtgctatc tgattggcat aagttggatt gagctctaaa aatacatagc
     2161 cgttttcatt cagtattttc gttgccaggg cggctattgt aatataaaac tcaagtggat
     2221 gctcctttac aaacaaggct gtgtgtggct cccacttcaa tacattggga tgcatctcct
     2281 tggcttcatg ctgcgcaata tatggtggat ttgaaacaat tatatctacc ttcgactgta
     2341 gtgtttctgt gcctgaatca aacaccgatt cgttaacaaa cgtaaccgaa gcacccaaat
     2401 cagaagcatt cttaattgca acgtttatag cttctgctga aatatctgtt gccttcacca
     2461 aagcttttgg aaacagcaat gacaactcaa tagcaatgca acccgaacct gtacctattt
     2521 caattattga tccaacatgt tctgatttca gattctgttt cataagcaga atcaattctt
     2581 ctgtttccgg tcgtgggata agcacacttg agttaacttt ataaatatga ttacagaacc
     2641 atgcctgacc taaaatatat tgtattggtt cgtaattgcg taaacgttcc agatattgat
     2701 gaattaaggt ttcctgtgac acatttacta ttacagtatt gcttgtctgg tagtctgaac
     2761 cgaaaacatg ttctataacc aacctggtga tagcaacact ttcgcttttg tcatagatta
     2821 aatcaacctc tgcctgaaaa gaactgataa tttcttttaa tgtcattgtt gcaaagctaa
     2881 ttaaacgcat catttatttt aattttgcca caacgaatgt ttatgacttt tgacgaaaca
     2941 ataatgcatc gctgtcttga attagctaaa atggcatcag gacaaacgat accaaatccc
     3001 atggtgggca gtgttcttgt ttttgaaaat aaaattattg gagaaggcta tcatcaacgc
     3061 gcaggagaag cacatgcaga agtcaatgca ataaacagtg tagaaaataa atctctaatt
     3121 ccgctttcaa cactttatgt ttcattagaa ccctgttcac attacggtaa aacaccgccc
     3181 tgctgtgagc gaattgttca ggaaggaatt aaaaaagtta ttgttggcac cctcgataca
     3241 accactaaag ttaatggtaa aggaattagc tatcttcagc aaaacaatgt ggaagtcctt
     3301 acaccaatgc taaatgatgc atgtcgtttt tcaaacagaa gattttttac ctatcatgaa
     3361 aagcacagac cttatatcat attaaaatgg gcagaaagtg ctgatggttt tattgctcct
     3421 gaaaataccc aacaacaata ttctataagt aacaccttta gtaaaacact atcacataaa
     3481 tggcggacag aagagcaggc tatattggta ggaacaaaaa ctgctatcat tgacaaccct
     3541 caactgaata gcagactatg ggagggaaat gctccaataa gagttgtcat cgacaaaaac
     3601 ttagctattc ccacatcaca tcatttgttt gatggttctg ttaaaactat tttaataaat
     3661 cagaagaaag aaattagtga gaacaacatt aattacataa aaattaactt tcaggaaaat
     3721 cctgcaactc aaattctaaa tgtattatat cagcttaaca tccaatcagt aataattgaa
     3781 ggtggaacaa aaacacttca attatttatt caaaataatt tgtgggatga aggaagagtg
     3841 tttactgcta agaaacaatt aatccgtgga atcaaggcac ctgaattttc aatttcacct
     3901 tccgaaataa caactattgc agacgataca ttaaaaatat tttacaacgt atgacagtta
     3961 ccgttttcat ggcagtactg tttgcctctc ttctctttgt catacttaaa ttattcgctc
     4021 gttataaaat cagcaatatc catgcattaa catataatta ttttacagca ggtatttgtt
     4081 ataccttgta ttcaaagcaa accttcagct caattgccgg atttgtggca agctatttcc
     4141 cttattatct tctgagtgga tgcttgttta ttggcgtatt ttatctcact gctttatcat
     4201 cacaaaagaa tggagttgct tcaacaacca ttgccagcaa aatgtcgtta gtaattcccg
     4261 ctttgcttgc tgtttacctg tacaatgaaa ctttctctct gcttaaaaca atagggttgt
     4321 taatggcact ttcagcagtt tatttatcag ctaggggaag taaccctgaa tcatcagaat
     4381 ccggttggaa actaccgcta ttggtttttg caggtgcagg tttagttgat gcttcaatca
     4441 aatatacaca gcatgtcttt actgacaatg caggcagtag tggtttccta tcgttagtat
     4501 ttttctctgc tgcaattatt ggcagttttg ttcttttatt taaaaaaaac aaatccatag
     4561 gaggaaacaa attcagcaat atcattgcag gaattatttt aggtttcttt aattttattt
     4621 ctctttacta tatgattgca gcacttcagg aatttcatga ggacagtggt aaagcattta
     4681 tcataataaa ctcaggtgtt gtgctgttga actttttact ttctgttatt ttatttcaag
     4741 aaaagaaaaa tacacaaagc cgtgttggtg ttacccttgc agtagcagca atcattatat
     4801 tgtcacaata aatgtttgaa acaaactata aatcaattat tggtcactct gaaggtgttt
     4861 tcagagataa aggcagcagg tttctggctt ttgcctacta tgttgaaacg gaaaatgaat
     4921 gtaaatctgt tcttgaacga ataaaaaaag agcatcctaa agcaaatcat cactgttatg
     4981 cttatcgctt agggccttat aaaaatattt tccgctggta tgatgacagg gagccatcca
     5041 acactgccgg tcgccccatt tacgcagtca ttcaatctaa cgacttaaca aatattctta
     5101 ttgttgtagt ccgttacttt ggtggcactt tacttggtgt ccccgggttg atacaggcct
     5161 acagaagtgc tgcagaagca gccatagagc aaaacacaat tattgaaaaa gaaatagaag
     5221 aagaatacga gcttcaaatg tcgtttgaag aaatggcaga ggtgatgaaa attgtcaaac
     5281 aacataaagc gactttcact caaacttcta ctgcagacac tttgacttta ttagtaaaaa
     5341 caaaaaaaac tttttctgat cattttctaa aagcactaac ggaaaattac ctgttgaaag
     5401 aaaaaataaa gataaaagcg ctcttctgat tttaatataa atcatattta agcaagcgac
     5461 actcaatagt tccattgtag aacggaatgc gggctgaagt tcgcagccct actttctttg
     5521 caagtgtcag gtcaccggta aaaataaaac ctgtctttcc cttacatttt tgtttgaaga
     5581 aatcaccaat tgacttataa agagcttcca aatccatatt ctcccccaat ctttcaccat
     5641 aaggaggatt aattacaata acaccgttgc cttcaggaat taaagtttct gcaaaatcac
     5701 atttattcat ttcaataaaa acatcaactc cggcacgttc tgcattttct cgtatggcct
     5761 ttattgcata accattgtta tctgttgcaa tgatggcagg catgttgtca aaattacttt
     5821 tcaatgcaag ttcactgcga agcatctcaa actttttttt gttaaagtcg aacaagtgca
     5881 tgtatccgaa attcgatctg gacagcaaag gcaatttatt tcctgccatc aaagctgctt
     5941 caattgcaaa ggtgccacta ccacacatgg gattaataaa cgcacttttc ttatcccatc
     6001 ctgttgcttt tataattgct gctgccagat tttcttgcac aggtgcctta aatggattct
     6061 gacggtaacc atgtttgttt aattgttcac cggaagtgtc gagatatacc catgctttat
     6121 tttcctgcca atgcagatga ataaccacct cactgcgtaa tggtcctgaa tcgggacgaa
     6181 tattataatg actttgaaaa aaatccacca ctgcatcttt acatagttga ttcaaaaaca
     6241 tactattgtt gattgatgga tgattagttt gagacgtaat ggtgatgtac tttttctccg
     6301 aaaaataatc tgaccacctg atgcggctaa gtttattata caattgtttc tggtctgttg
     6361 cagtaaattt atcaaccaac caaagtaccc tgcttgctgt agtgagttga aagtttaact
     6421 ccatacaacg ttcaaatgct gctttaataa acaatcctgt ataattttca tcaaaaggtt
     6481 tgtaacctaa ggcatccaat tcctgcttta accaaggtac aacatgtggt gcacaactga
     6541 taaaaaatgt ctcctgacct ttcattgaaa gtttaatttc gcgcaaaggt aagttttagt
     6601 ataatcagca ttgaaaaagt ttaacaccta atattaaaaa ctcagcttaa tatattaaac
     6661 atttcaattc aacgttgtaa tattgtcttt gtactggctt aactttgcac taatgacaga
     6721 aaataaaaaa ccaaacaatc aggaacttat tgctatgata agcctgcttg atgatgaaga
     6781 tgaaacggtt tacgaatcac ttctaagtca ctttttggaa ataggtaaac cagctattcc
     6841 tttgttagaa gaaaaatgga gtcaatcact tgatgcccta ttacaaacac gcattgaaaa
     6901 tattgtacat aaaattcagt ttgaagcagt aaaaacagaa ttgacaaact ggaaagaaca
     6961 caatcaaaag aatttactta ccggtttaat agctattgca cgttatcaat atcccgactt
     7021 agatgaagac tccataaatg tattttttag cgaagtaaga agacgtgttt gggatgacct
     7081 tcaacccaac gacacccctt tagaacaagt cagaattctt aatcaggttc tttttgtaga
     7141 gtttggtttt aacggcaata cagctaattt tcactcacct caaaactcat ttataaatac
     7201 agtaattgaa agtaaaagag gtaaccctat tctattaagt gtaatttatg caatcgttgc
     7261 tcaggatctt gacattcctg tttatggtgt aaatctgcct gaacattttg tattatgcta
     7321 tcagacatta cataaagaca aatttgcaga gtatgctttt cctgaagata atatattgtt
     7381 ttttatcaat gcattcagcc gaggctctgt attcgggaaa gatgaaattg atctttattt
     7441 gaggaaactg aaactgccgt ctgataaaaa atttattaca ccttgcagca atacagatat
     7501 tatcagacgc attttgcaca acttgaattt ttcgttcaac aaatcgggcc ttacagagaa
     7561 atctgaggaa atagaaatac ttcttgcttt actagaaaaa taaaaccgct tcctgccagt
     7621 tgggaagcgg ctaattcaaa taatatgtaa ttacaataca cttattcact agcaatataa
     7681 atattcagtt ggtggaaaaa ctatctattc agaagtaata ttgcatttta aagcagatgc
     7741 tttaccatta aaaaatcaat ttggtattca tcatatataa gaaaataaat tacgctcttc
     7801 tcttataact ccttacgctt ttgcgaagta caatactatt catccattca gcgttgacta
     7861 atatcctgtt tatcaaaagc accataagca tcaaaaaaag tagttgcagc tataattgaa
     7921 aatattgctg catcatttat agttgccttt aatattttta gatctaactt tcaatacata
     7981 aatataggaa aaaaataagg ggcagtgctt tggcactgcc ccttattaat ttatttaagc
     8041 aaaaactact ccactaccac tctcacctga ttgttgctgc cattggtttg tacat
//
LOCUS       DAOQNP010000100         8079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3300_length_8079_cov_9.952393, whole genome shotgun sequence.
ACCESSION   DAOQNP010000100 DAOQNP010000000
VERSION     DAOQNP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3300_length_8079_cov_9.952393"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1509
                     /locus_tag="PKY09_11260"
     CDS             73..1509
                     /locus_tag="PKY09_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNB13476.1"
                     /translation="MAENENFQKIKHLYNRAGFGLHYSELQKLSHTKLNKLVADMIEK
                     AKSDVPITTIAPDHVKSVMLKNKGLTKKEGLSPEQLKERQKLLVSLNKQLKNLNAEWV
                     FRMMNTDSPLREKMTLFWNNHFACREEGNPYFAQVLNNIQRKLALGDFRMLLIEVSKS
                     ASMLNFLNNQQNKKGRPNENFARELMELFTLGRGNYSEKDIKESARAFTGWAHDAAGN
                     FEFNPKNHDNGIKTFFGKEGNFNGEDIIDMILQKPEAAVFIARKAYRFFVNDVPDENH
                     VQELGNHFYKSKYDIAAMMNKMFTSDWFYAPENMGNKIKSPVEFIVSLSRQFNVTYKR
                     QEIILQLQRGLGQTLFYPPNVAGWAGGRNWIDSSSLMLRLTIPSQILNDSSLNFSAKT
                     DPDDENIVAMKHQDDVVVEAKKKASGIEINWDNFWSDFPKDYSVQQIAAFVLQCKPST
                     QLNQIINSSVDKKNAIIKTVSSPEYQLC"
     gene            1519..>2444
                     /locus_tag="PKY09_11265"
     CDS             1519..>2444
                     /locus_tag="PKY09_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNB13477.1"
                     /translation="MTTMKRRDFLKQSVFASAGSMLIPAFLKPFELIAKSNLSGHKNL
                     VIIQLSGGNDGLNTIVPYGNDVYYQLRNTIAIPQSEIIKLNAMQGLNPALAPLKEIYD
                     QGWMSIINNVGYPNPNRSHFRSMDIWQSASDSNQFLSTGWIGRYLDSNCQMCKYPYNA
                     IEVDDTLSLTLKGDTKKGIALQNPDKLFRTTNTPFFGKLIEHDKEHLDEDNLGYLYKT
                     MIETYSSAGYIKETTKNYENNFVYPESAFAKKLKTVARFLLSGLDTRVYYVSLSGFDT
                     HVNQIAQQKKLFTQFSEGMNAFVKELNNNKKL"
     assembly_gap    2445..2544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2545..3368)
                     /locus_tag="PKY09_11270"
     CDS             complement(<2545..3368)
                     /locus_tag="PKY09_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNB13478.1"
                     /translation="MESIDFLQREIEYEIKSLHFGHEPANLYQPMEYIVELGGKRMRP
                     LLVLIGAGLFEANYQKAMKAAIGIELFHNFTLLHDDIMDKATLRRNKPTVHEKWTADI
                     AILSGDALFVRASEYIMQVDDAVLRPVMQLFYKTAMQVCEGQQYDMDFGRLREITPED
                     YIGMITLKTAVLPACCLATGAIIAKASEEDKLSLYEFGKNIGIAFQIQDDLLDAFGDD
                     TKFGKRPGGDIIEGKKTFLFSEACRLLNGKEHLDFLTLYNSHNLSNHEKITAVMQV"
     gene            complement(3378..4322)
                     /locus_tag="PKY09_11275"
     CDS             complement(3378..4322)
                     /locus_tag="PKY09_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNB13479.1"
                     /translation="MKSKILVTGGAGFVGSALAEKLADNADNFVVVVDNLLTGSMRKV
                     PASKHNNVKFIKADVNDFSDISSIFYAYAFDYVFHYAAVVGVKRTLDHPSWVLKDLTG
                     LKNILDLSKNTGVKRVYFSSSSEVYGEPVEYPQNETTTPLNSRLPYAIVKNLGEAYLR
                     AYKQEFDLDFTIFRFFNTYGPKQSTDFVMSKFIEAALSNKDITIYGDGSQTRTFCYVD
                     DNVEACLNAFYTHRFVNDVINVGNDHETSILELAQLIKKILNSKSNIVHLPALKEGDM
                     TRRCPDIGKMKILLNRELTPLEIGMERVIEVFRNKKGK"
     gene            complement(4337..5206)
                     /gene="rfbA"
                     /locus_tag="PKY09_11280"
     CDS             complement(4337..5206)
                     /gene="rfbA"
                     /locus_tag="PKY09_11280"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNB13480.1"
                     /translation="MKGIVLAGGSGTRLHPLTLAMSKQLMPVYDKPMIYYPLSVLMMA
                     GIREILIISTPHDLPHFKKLLGSGEQIGCRFEYAEQAVPNGLAQAFVIGEKFIGSDSA
                     ALVLGDNIFYGQGLGRILADNNKISGGLVFAYHVSDPERYGVVEFDANYKALSIEEKP
                     QKPKSNFAVPGLYFYDNSVIEVAKNLKPSERGEYEITDINKHYLQQGKLKVQLLERGT
                     AWLDTGTFNSLLQAGNFVNVIEERQGLKIGCIEEVAYRMKFIDKEQLRKVAEPLTKSG
                     YGNYLIKIIEQNL"
     gene            complement(5509..6090)
                     /locus_tag="PKY09_11285"
     CDS             complement(5509..6090)
                     /locus_tag="PKY09_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13481.1"
                     /translation="MFNNLFLFISVLFFVNCGNDTKSQNSIGILFKDSLNTLSIYIPK
                     SFSLIDSKYEKEILGDKYELFSASFRGEEIKITDFIKEYNYIGLSVNFNSYMKDLAKV
                     DSLEELASSFDKEIINSIKSNYQSEKKNYFLIKQKFYNSLGTPNIQLIETYCLHKSDL
                     FFLQATFYYTNEAQPEKNYKEIIVVLESLEFTH"
     gene            complement(6080..7099)
                     /locus_tag="PKY09_11290"
     CDS             complement(6080..7099)
                     /locus_tag="PKY09_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNB13482.1"
                     /translation="MNSKEIKYLTFEGGGGKCIAYLGAIKALEEKKILPLSHVNTVNF
                     KTDDLDINIPQNNLFGVSGSSGGALIAFFLSMGLDLHEMESLFNEPYDKDNNQFITFF
                     DYPESNLKGLNQTIQYYRSVIQKKEMKNGKPKYFSAAFTPDFSVVDAVSLSMSIPFLF
                     RPYVIESEVNLDSNLPADYNKDYLGLYVDGGMLNNYPLHAFDYRPAKSINYQGDFANY
                     FLASPNISLSATEFCDCILGIRLQNSVKEYEVSDDFKEGNSFIIRDFIGQLYSTILYP
                     SEDGQIRSSLENNLSISIDVTDLELLDFSNPLINRKYRKLNALAEKQEKVIENAYKKI
                     YENVQ"
     gene            complement(7096..7932)
                     /locus_tag="PKY09_11295"
     CDS             complement(7096..7932)
                     /locus_tag="PKY09_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13483.1"
                     /translation="MRKLFLAITALSIFFISCNSSPNQEKNKPVAPVFADTSVQLFSK
                     ENPVINLNIKYINDAYKFEKQLGSKISYFPRGVDLCDHFPGDNDPWWDSIIRRPTSHL
                     ENPQYVIFQRLEKYDFPLNVIYEYNPKTRIVSYINYEWAPHWIHYDNDFDLDTAIENY
                     KIDFADMHFYDTLFVKLFNVVSPTFHDTLTGLDIILERKYYENIVDKNIETGLNNKMV
                     LESYNTPSYWSNNDYIVRLQLTQSLEYINCSVNNNTINKKMKYRGLILSFLLKKNAKY
                     DK"
BASE COUNT         2563 a         1426 c         1407 g         2583 t
ORIGIN      
        1 ctttcagttt ttatttttgt tcagactaaa cctttgttac aatatcttgt caaatactaa
       61 gtttggttta ttatggctga aaacgaaaac tttcaaaaaa taaagcacct ttataaccgt
      121 gcaggttttg gattacacta ttctgagttg caaaaattaa gtcatacaaa gctgaataag
      181 ttagttgcag acatgattga aaaagcaaaa tctgatgttc ctattacaac cattgctccc
      241 gaccatgtaa aatctgtaat gcttaaaaat aaaggactta ccaagaaaga aggcttgtca
      301 ccggagcaac taaaagaaag gcaaaaactt ttagtgagtc tgaacaagca acttaaaaac
      361 ctgaatgcag aatgggtatt ccgcatgatg aatactgaca gcccgctgcg cgaaaaaatg
      421 actttattct ggaacaatca ctttgcctgc cgcgaagagg gaaatccata ttttgctcag
      481 gtacttaata acattcagcg taaacttgct ttaggcgatt tcagaatgtt gttgattgag
      541 gtttcgaagt ctgcctctat gctgaatttt ttaaataatc aacaaaataa aaaaggcaga
      601 cccaatgaaa actttgcgcg tgagttaatg gagttgttta cacttggtcg tggtaactat
      661 tcagaaaaag atattaaaga atcggcacgt gcctttaccg gctgggcaca tgatgcagca
      721 ggtaactttg agttcaaccc caaaaatcat gataatggta taaaaacatt tttcggaaaa
      781 gaaggcaact tcaatggtga agatattatt gatatgattt tgcaaaagcc cgaagcagct
      841 gtttttattg caagaaaagc ttatcgtttt tttgttaatg atgtgcccga tgaaaatcat
      901 gtgcaggaat tggggaatca tttctacaaa agcaagtacg acattgctgc aatgatgaat
      961 aaaatgttta catcagattg gttttatgct ccggaaaaca tgggaaataa aattaaatct
     1021 cccgtagagt ttattgtctc acttagtcgt cagtttaatg ttacttacaa gaggcaggaa
     1081 ataattttgc aactgcaaag aggtttaggg caaactttgt tttatccgcc caatgtggca
     1141 ggatgggctg gcggtcgcaa ctggatagat agttcatcac tgatgttgcg tttaacaatt
     1201 ccttctcaga ttcttaatga tagcagttta aatttttctg ccaaaaccga cccggacgat
     1261 gaaaacattg ttgcaatgaa acatcaggat gatgttgttg tagaagcaaa aaagaaagct
     1321 tcaggaatag aaattaactg ggataatttc tggtcagatt ttcctaaaga ttattcagta
     1381 cagcaaatag cggcttttgt tctgcagtgt aagccaagta cacagttgaa ccaaatcatc
     1441 aacagctctg ttgataagaa gaatgcaatc ataaaaactg taagctctcc ggagtatcaa
     1501 ttatgctgaa acagaaaaat gacaacaatg aaaagaagag actttttaaa acaatcggtt
     1561 tttgcaagtg caggcagcat gttgatacct gcatttttaa aaccatttga gcttattgca
     1621 aaaagtaatt taagcggaca taaaaatctt gttatcatac aactttcagg tggcaatgat
     1681 gggttaaaca ccattgtgcc ttatggaaat gatgtgtatt atcagttacg aaacactatt
     1741 gccattcctc aatcagaaat tattaaactg aatgccatgc aggggctaaa tcctgcatta
     1801 gctccactaa aagaaattta cgatcagggt tggatgagta ttatcaacaa tgttggctat
     1861 ccgaatccta accgttcgca ctttcgttct atggacatct ggcaatcagc aagcgactct
     1921 aatcagtttc tttctacagg atggattgga cgctatttag actcaaactg ccagatgtgc
     1981 aagtatccct ataatgcaat tgaagtggac gacaccttat cattaacttt gaaaggtgat
     2041 actaaaaaag gtattgcact gcagaatccg gataaattat ttcgcactac aaacacaccg
     2101 ttttttggta agttgattga acatgataaa gaacatcttg acgaagataa tcttggttac
     2161 ttgtacaaga ccatgattga aacatattca tcggcaggtt atattaaaga aacaacaaag
     2221 aattacgaaa ataactttgt gtatcccgaa tcggcatttg caaagaaatt aaaaaccgtt
     2281 gcacgttttc ttttatcagg acttgacaca agagtttatt atgtttcact cagtggattt
     2341 gatactcacg taaaccaaat tgcacagcag aaaaaattgt ttactcagtt ttctgaaggc
     2401 atgaatgctt ttgtgaaaga gttgaacaac aataaaaaac ttgannnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnaacacc tgcatcactg ctgtaatctt ttcgtgattg
     2581 gacaaattgt gcgagttata cagggtcaga aaatcaagat gctctttgcc attaagcagc
     2641 ctgcatgctt ctgaaaaaag aaatgttttc ttaccttcaa taatatcgcc accgggtcgt
     2701 ttgccaaatt tagtatcatc accaaaagca tctaacaaat catcctgaat ctgaaatgca
     2761 attccaatat tttttccaaa ctcatacaag ctcagtttgt cttcttcaga ggctttggca
     2821 attatagcac ctgttgcaag acaacatgca ggaagcactg ctgtttttaa cgtaatcatg
     2881 ccaatataat cttcaggagt aatttcgcgc aatcgtccga agtccatgtc gtactgctgc
     2941 ccttcgcaaa cctgcatggc tgttttgtaa aacaattgca tgaccggtct tagtacggca
     3001 tcatcaacct gcataatgta ttcacttgcc cttacaaaaa gtgcatcacc ggaaagtatt
     3061 gcaatatcgg cagtccattt ttcatgtact gttggtttgt ttcttcgcaa cgttgcttta
     3121 tccatgatgt catcatgcag cagtgtaaaa ttgtgaaaca attctatacc tattgcagct
     3181 ttcattgctt tctgataatt tgcctcaaac aagcctgcac ctatgagaac caataatggg
     3241 cgcatacgtt tgccaccaag ctcaacgata tattccattg gctgatatag attggccggc
     3301 tcatgaccaa agtgcaacga ctttatctcg tattcaattt cgcgttgcag aaagtcaata
     3361 ctttccatta agataaatta ctttcccttt ttatttctga aaacctcaat cactctttcc
     3421 attccaattt caagaggtgt taattcgcga ttgagtaata ttttcatttt gccaatatca
     3481 ggacatctgc gtgtcatatc accttctttt aaagcaggta aatgcacaat gtttgatttt
     3541 gaattcagaa tttttttaat caactgcgcc aattccagaa ttgaagtttc atgatcgtta
     3601 cccacgttga tgacatcatt cacaaaacgg tgtgtgtaga aggcatttaa gcaggcttca
     3661 acattatcat caacatagca gaaagttcgt gtttgtgagc catcaccata aatagtaata
     3721 tctttattac tcaatgcagc ttcaataaat tttgacatga cgaagtctgt actttgcttg
     3781 ggtccataag tattgaagaa gcgaaaaatg gtaaagtcaa gatcaaactc ctgcttgtag
     3841 gcacgcaaat aggcttcgcc aagatttttt acaatggcat aaggaagtct tgagtttaaa
     3901 ggtgttgttg tttcattttg cggatactca acaggctcgc cataaacctc gctgctgctg
     3961 ctgaagtaaa cacgttttac cccagtattc tttgataagt caagaatgtt ttttaatccg
     4021 gtaaggtctt ttaataccca tgatggatga tcgagtgtgc gttttacacc cactacagct
     4081 gcatagtgaa aaacataatc aaatgcatag gcatagaata tggatgatat atcgctgaaa
     4141 tcattcacat cagctttaat aaatttaaca ttgttatgtt ttgatgccgg aactttccgc
     4201 attgaaccgg ttaaaagatt gtcaaccaca acaacaaagt tgtcggcatt gtctgctaat
     4261 ttttctgcca atgcacttcc tacaaatcct gctccacctg ttactaaaat tttacttttc
     4321 atatattatg tttcgcttat aaattttgtt ctattatttt tatgaggtaa tttccgtagc
     4381 cgctttttgt caatggctca gctactttac gtaattgttc tttatctata aatttcatgc
     4441 ggtaggccac ttcttctatg cagccaattt ttaaaccttg tctttcttca ataacattca
     4501 caaagtttcc tgcttgcagt aatgaattaa atgtaccggt atcgagccat gctgtacccc
     4561 gctcaagcag ttgcactttt aatttaccct gttgcagata gtgtttgttt atgtcagtaa
     4621 tctcatattc accacgttca cttggcttta aatttttggc aacttcaata accgaattat
     4681 cataaaaata caatccgggg acggcaaagt tcgatttggg tttttgaggt ttctcttcta
     4741 tggaaagtgc tttgtaattg gcatcaaact caactacgcc atatcgctcc ggatctgaaa
     4801 cgtgataggc aaaaaccaat cctccactga ttttattatt gtctgcaaga atacgtccta
     4861 atccctgacc ataaaaaata ttatcaccaa gaaccaacgc tgcactgtca ctgccaataa
     4921 atttttcacc aataacaaaa gcctgtgcca gtccattagg aactgcctgt tctgcatatt
     4981 caaaacggca gccaatctgc tctccactgc ctaaaagttt tttaaagtga ggcaagtcat
     5041 gtggggtgct gatgattaaa atttctctta ttccggccat catcaatact gatagcggat
     5101 aataaatcat aggtttatca tataccggca taagctgttt gctcatggcc aatgtaagtg
     5161 ggtgcaggcg tgtgccgcta cctccggcta aaacaattcc cttcatcgta tggttgcaaa
     5221 agtgtatttg tacgtaaaat tacgtcaggt ggtttctaaa aatttaaggc tgtaaaggta
     5281 ataatgttat aaaattacag aaatacagtc tttgtgctta ggtattatta tttctttaag
     5341 acaaatagga aataataaat tccggaaaac cccactcgtc ctcgtctgaa gttaagtacg
     5401 tgcgtaaggg acgaggatgt gtggggctct ataacaggtg ttaatcgcat tctcgtctcg
     5461 cgcatacaac gctacccttc agacgaggac gagtggtgtt tgctgctgtc aatgtgtgaa
     5521 ttccaaactt tctaaaacga ctataatttc cttataattt ttttcaggtt gggcttcatt
     5581 tgtatagtaa aacgttgcct gtaaaaagaa caagtcggac ttgtgaaggc aataagtttc
     5641 tattaactga atattaggag tgcctaatga attatagaat ttttgcttta tcaagaaata
     5701 attttttttt tctgactgat aattagactt aatactgtta ataatttctt tatcaaaact
     5761 tgatgccaat tcctctaagc tatcaacttt tgctaaatct ttcatatatg aattgaagtt
     5821 tacacttaaa ccaatataat tgtattcttt tataaaatca gtaattttaa tctcttcccc
     5881 tctaaaagag gcagaaaaaa gttcatactt atcaccaagt atttcttttt cgtacttact
     5941 atcaattaag gaaaatgatt tgggaatata aattgacagc gtgtttaaag agtccttaaa
     6001 aagaatgcca atggaatttt gagatttagt atcattaccg caatttacaa agaataaaac
     6061 cgagataaat agaaacaggt tattgaacat tttcgtaaat ttttttgtat gcattttcaa
     6121 taactttttc ttgcttttct gcaagagcat ttaattttct atacttacgg tttattaatg
     6181 gatttgagaa gtccagcaat tctaaatccg tcacatcaat ggaaatagat aagttattct
     6241 caagcgagga tcttatttgc ccgtcttcgc ttggatagag tatggtacta tatagttgcc
     6301 ctatgaaatc tcttataata aagctattgc cttctttgaa gtcatctgat acctcgtatt
     6361 ccttaactga attttgtaga cgaattccca gtatgcaatc acaaaattca gtagctgata
     6421 aactaatatt tggagaagcc agaaagtagt tagcaaaatc accttggtaa ttgatgcttt
     6481 ttgcaggtct gtaatcaaaa gcgtgaagag gataattatt gagcattcct ccatcaacat
     6541 acagcccaag ataatcttta ttataatcag caggaagatt tgaatcgaga ttaacttctg
     6601 attcaatcac gtatggcctg aatagaaaag gaatactcat tgataaactg acggcatcaa
     6661 caactgaaaa gtcaggagta aaggctgcag agaaatattt tggtttgccg tttttcattt
     6721 cttttttttg aatgactgaa cggtaatatt gaatggtttg gttaagacct tttaaatttg
     6781 attcgggata atcaaaaaat gttataaact ggttattatc tttatcatag ggttcattaa
     6841 ataatgattc catttcgtgt aaatcaagac ccattgaaag aaaaaaagct attagtgctc
     6901 ctccggaaga accactgact ccaaataaat tattttgtgg tatattaata tcaaggtcat
     6961 ccgttttgaa gttgactgtg ttgacatgag ataaaggcag aatttttttt tcttccaatg
     7021 cttttattgc gcctaaataa gcaatgcatt tgcctccgcc accttcaaaa gtaagatatt
     7081 ttatttcttt actgttcatt tatcatactt agcatttttt ttcaatagaa aagataatat
     7141 caaccctctg tatttcattt ttttatttat ggtattatta ttaacgctac agtttatata
     7201 ctctaaactt tgggttaatt gaagtcgaac aatataatcg ttattactcc aataacttgg
     7261 agtattatat gactctaaga ccattttatt gtttaagcct gtttcaatat tcttgtcaac
     7321 gatattttca taatattttc tttcaaggat tatgtccaag ccagttaagg tatcatgaaa
     7381 tgttggagaa acaacgttaa ataatttaac aaataaagta tcataaaaat gcatatcagc
     7441 aaaatctatt ttataatttt caatagccgt gtcaaggtca aaatcattat cataatgaat
     7501 ccaatgagga gcccattcat aattaatata agaaactata cgggttttgg ggttgtattc
     7561 gtatataaca ttaagtggga agtcatactt ttctaaacgt tgaaaaataa catattgagg
     7621 gttttctaag tgggaagttg gtcttctaat aatggaatcc caccaagggt cattatcacc
     7681 aggaaaatgg tcgcataaat caactcctcg agggaagtag cttatcttag aacctaactg
     7741 tttttcgaat ttatacgcat cattaatata ctttatattt aagtttatta caggattttc
     7801 tttagaaaat aactgaacag aagtatcagc aaaaaccgga gcaacaggtt tgtttttttc
     7861 ctgattggga ctggagttac aggaaataaa aaaaatactt aatgccgtaa tggcaagaaa
     7921 gagtttgcgc atggtaatta atgttatggc aataactccc actcgtcctc gtctgaagtt
     7981 aagtacgtgc gtaagggaca aggatgtgtg gggctctata acagtgactc gtcctaaaaa
     8041 aagtttacag tttaattgat taatcctata ataagttta
//
LOCUS       DAOQNP010000101         7868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3440_length_7868_cov_8.123128, whole genome shotgun sequence.
ACCESSION   DAOQNP010000101 DAOQNP010000000
VERSION     DAOQNP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_7868_cov_8.123128"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKY09_11300"
     CDS             complement(<1..901)
                     /locus_tag="PKY09_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNB13484.1"
                     /translation="MEVTRLIDLFPYQLSRFPKADALAGKQDGVWKKYATAEIIETVN
                     YLSYGLLHSGIKPGDKIGIIANNRPEWNFVDHACLQISVADVPLYPTISENDLKFTLN
                     QSAVRYLFVSNADLYKKVSQIKSELPALEKIYLFDHYQDLPHWSELLELGRQHPKQEE
                     VKQIRDSIKPNDLATIIYTSGTTGNPKGVMLSHNNLVSNFKFCDVLCPFHESWKALSF
                     LPLNHVYERMLSYLYVYFGVSIYYAESLDTIADNLKEIKPQIFVTVPRLLEKVYDKIV
                     SKGAALTGIKKALFFWALNLGLRY"
     gene            970..1353
                     /locus_tag="PKY09_11305"
     CDS             970..1353
                     /locus_tag="PKY09_11305"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNB13485.1"
                     /translation="MNNHDLLNNSKPLLIFDGSCAFCTSCIRFVKKHDVNQQFDYADN
                     NQIVTKLILAQHFIDSETLNTVVLIENNNVHIQSDAVIKTLLFLGGRWKFLGKFMIVF
                     PASFSNKVYNTIAANRNRFNSHCKI"
     gene            1350..2348
                     /locus_tag="PKY09_11310"
     CDS             1350..2348
                     /locus_tag="PKY09_11310"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HNB13486.1"
                     /translation="MKTDFTEQTGITYPIIMAPMFLVSNEAMIQSAIQTGIMGVFPTL
                     NYRKQDELDALIKRLHLFKSEKKGSFGVNLIVQKTNPLYSSHLQICVENKVPFYITSL
                     GNPREVIDAAHTYGAKVYCDVTNITHAKKCAQLGCDGFIAVGQGAGGHAGPYAQTVLI
                     PALRKHFHTIPVIAAGGIATGDGIAAMMALGAAGVSIGTRYIVSYEAKVSDEYKNSVL
                     NSGMDNIVMTERLSGTPCNIINTPYAKKIGFKQNWLEKWLSNNSRTKKYFKMLVQYKG
                     LQALEQAIKPGNYKTLWTAGQTVELVDDILSCDEITARLIKETYQSVENLNNKFKS"
     gene            2497..3990
                     /locus_tag="PKY09_11315"
     CDS             2497..3990
                     /locus_tag="PKY09_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13487.1"
                     /translation="MTFKRIFLFIWLSVFFHIAQAQKVTYAQQELDAYSYDYIKTAGY
                     NDNSFFVFQSNLPFENDRDRVGFKSRKYRVTCFNRNLSLKWSKKIELKGEKEKLETIT
                     VINGQVALIKSSFEGKDNQISVYITTLDDNGNESSTRKDIGTILFNRTSNLDKIKVIA
                     SKGNNYFGLIQNEKKQNGFQQLHCLIIDSDFKLVRQLQFEVPYPEKNFNYENWLLTDD
                     ADFILEGSYYSKEKSENRKRWDAYKLFVSSNGNSGAIEYAINTSNLILSSSAIAYDAI
                     NKKLVLAAFYTDNVSTKSGIIYASLGTAANDSLLMIRQPIGDATLTRMFAGQNLDRSS
                     AGIENLSIEKLILRNDGGAVLVTEVSYVTDYSYYDYFTQNYIRNFEYHFNNVITFSIN
                     PDGTIDWDAVLRKEQVSTNDYGKYSSFALAMTESDISLLYNTRTDRNNGVAYFGIKRN
                     GETESKEIIKPEERILLVPFAAKQISTDEIVVPCWNRKKMLMAKITF"
     gene            3990..4769
                     /locus_tag="PKY09_11320"
     CDS             3990..4769
                     /locus_tag="PKY09_11320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB13488.1"
                     /translation="MPGALSLALQYISYYVRAWNKHNVHSPFVFKLTTEVIHPDKKLA
                     AFIPIEQQRKLLKQNADKILFTELGAGNKSNQRSVSNIAAKSLKSPRYARLLYRLTNH
                     FQPENIIELGTSLGITTGYLALSGCKNIYTIDGNKTVSDIAKATLTSVKLSSNVKFIN
                     GNFDESLPATLNTLNKVDFVFFDGNHRYQPTLNYFQQCLTKAHDKSVFVFDDINYSHE
                     MKKAWKEIKQHPSVVVSIDLFMMGIVFFDTDLLRQHFVIRY"
     gene            4828..5547
                     /locus_tag="PKY09_11325"
     CDS             4828..5547
                     /locus_tag="PKY09_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB13489.1"
                     /translation="MPQSIISLSDISRLYKVGTETIYALRSVSLNIFKNEYVALMGSS
                     GSGKSTLMNILGCLDTPSSGSYHLNNISVSGMTDNELAEVRNKQIGFIFQTFNLLPRS
                     TALENVALPLVYAGVSKEERLKRAKEVLESVGLADRIMHKPNELSGGQRQRVAVARAL
                     VNHPSIILADEPTGNLDSKTSEEIMLLFEEIHQRGNTIIVVTHEEDIARHAHRIVRIK
                     DGLIEDDAINTQITKAKIIVS"
     gene            5544..6254
                     /locus_tag="PKY09_11330"
     CDS             5544..6254
                     /locus_tag="PKY09_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNB13490.1"
                     /translation="MNIKSIQSLLIVVVLLASCGVANSQKGKLLTPEVFEKMLHETPE
                     AQVIDVRTPEEFNDGHLKNALNMNVNSKDFENRAPYLDKAKPLFVYCYSGGRSAKACE
                     YFEKMGFKVIYDMEGGYSAWTDANLPVEGKKADKAGISLDNFALLTSGAKVLVDINAP
                     WCPPCVKMKPMLDSLDIAWKDKVKIVRLNKDENQLISKSLQVNELPTLLFYENGKLVK
                     RESGYKNAEQLKAMAQVE"
     gene            complement(6336..6482)
                     /locus_tag="PKY09_11335"
     CDS             complement(6336..6482)
                     /locus_tag="PKY09_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13491.1"
                     /translation="MPSATGGLKIDDVIEFSVHFPKKRTKEKDTNKANRFFLIIGFLF
                     LNFL"
     gene            6707..7207
                     /locus_tag="PKY09_11340"
     CDS             6707..7207
                     /locus_tag="PKY09_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13492.1"
                     /translation="MNISALAEAEKLLFIARQSLSEGKLPQAIDMLNKIVTDFPNFSK
                     AHITLADIYFNKLQDSTTGEEFYKKAIAIADMNTDAFTGYASLMLQQEKYPEALSLLN
                     KAMAIKGAPKDTIYMLLGKLYEMQSRVDDAMNNYKKCITSTLSNSVLEEAELALKRCE
                     VKKKYI"
     gene            7358..>7868
                     /locus_tag="PKY09_11345"
     CDS             7358..>7868
                     /locus_tag="PKY09_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13493.1"
                     /translation="MKRAFIFSLCFIVSHFISFAQLDQYTSEQNKFYWKNRKPTLNYW
                     QQDIYYNIKAVIDEKADIISGIEELTYRNNSPDTLKFIFMHLYQNAFVPGSYLDDLNK
                     ANQNKVRFGKYESQGLGIEITNVSTLSVNGVSINKQAEKTIDNTIMKIMLPEPLLPNH
                     DLVLKIDFKT"
BASE COUNT         2678 a         1377 c         1396 g         2417 t
ORIGIN      
        1 catagcgtaa gcccaggttc aatgcccaga aaaacaaagc tttttttata cccgttaacg
       61 ctgcaccctt tgaaactatt ttatcataaa ctttttccag caatcgtgga accgttacaa
      121 aaatctgtgg tttaatttcc tttaaattgt ctgcaatggt atctaaactt tcagcataat
      181 aaattgaaac accgaaatag acatagagat aggaaagcat tctttcgtac acatggttaa
      241 gtggtaaaaa actcaatgcc ttccaggact catgaaacgg acaaagtaca tcacaaaatt
      301 taaagttgct tacaagatta ttatgcgata acattacgcc ttttggattt cctgtagttc
      361 ctgaagtgta aataatagtt gccagatcat taggttttat gctatctcta atctgcttaa
      421 cctcttcttg ttttggatgt tgacggccta gttcaagcaa ttcactccaa tgtggcaagt
      481 cctggtaatg gtcaaataaa tatattttct caagtgcagg aagttcgctc ttaatttgac
      541 taaccttctt atataaatcg gcattggaaa caaataaata gcgcactgct gactggttca
      601 atgtaaattt taaatcattc tcgctaatgg taggataaag tggaacatct gctactgaaa
      661 tctgcaggca agcatgatct acaaaattcc actccggtct gttattggca ataattccaa
      721 ttttatctcc gggctttatt cctgaatgta acaatccata gctaagataa tttacagttt
      781 caattatttc ggcagttgca tattttttcc aaacaccgtc ttgctttcct gccaaagcat
      841 ctgctttagg aaaacgcgaa agctgatatg ggaacaaatc tattagtcgt gttacttcca
      901 ttctaaaatt agtttagttg tgctgcataa aaatataaaa agtatttaaa tgttttttac
      961 tattgcaata tgaacaatca tgatttatta aacaactcaa aacccttgct gatatttgat
     1021 ggcagttgtg ctttttgtac atcatgtatt cgtttcgtta aaaaacatga tgtcaaccag
     1081 caatttgact atgcagacaa caatcagatt gtaaccaaac taattttagc tcaacacttt
     1141 atagattctg aaactttaaa cacagtcgtt ttgatagaaa acaataatgt ccatattcag
     1201 agtgatgcag tcataaaaac cttacttttt cttggtgggc gttggaagtt tttaggcaaa
     1261 ttcatgattg tttttcctgc ctcattcagc aataaagttt acaatacgat tgctgcaaat
     1321 cgaaacagat tcaatagcca ttgcaaaata tgaaaaccga ttttaccgaa caaacaggca
     1381 ttacctatcc aattattatg gcaccaatgt ttttggtaag taatgaggcc atgatacaat
     1441 cagccattca aacaggaata atgggggtgt ttccaacatt aaactacaga aaacaagatg
     1501 agcttgatgc attaattaaa cgattacacc tgttcaagtc tgaaaagaaa ggtagtttcg
     1561 gtgtcaactt aattgtacaa aaaaccaatc cgctttatag cagtcattta caaatttgtg
     1621 ttgaaaacaa agtgcctttt tacattactt cattaggaaa cccacgcgag gtaattgatg
     1681 ctgctcatac ttatggagca aaagtttatt gtgatgtaac caatattaca catgcaaaaa
     1741 aatgtgctca acttggttgc gatggcttta ttgccgttgg acaaggtgca ggcgggcatg
     1801 caggacccta tgcacaaact gtattgattc ctgctttacg aaagcacttt catactattc
     1861 ctgtaattgc tgcaggaggt attgccaccg gtgacggcat tgctgcaatg atggcattag
     1921 gtgctgcagg agtaagcatt ggaacaagat atattgtgtc ttatgaagcc aaagtaagtg
     1981 atgaatacaa aaacagtgtt ttaaattcag gcatggataa tattgtaatg acagagaggc
     2041 tttccggcac accatgtaac attattaata caccttatgc aaaaaaaata gggtttaaac
     2101 agaattggtt agaaaagtgg ctgagtaata actccagaac taaaaaatat tttaaaatgt
     2161 tggtgcagta caaaggactc caagcattgg agcaggcaat taaacccggc aattacaaaa
     2221 ctttgtggac agcagggcaa actgttgaac ttgttgacga tatactgtct tgtgatgaaa
     2281 ttacagcgcg attaataaaa gaaacctatc aatcagtcga aaacctaaac aataaattca
     2341 agagttaaag catgcatgct tttcatgatt tggcgaaaaa aaagaatcgt tcttttaaaa
     2401 ttctcagtat tctgttacat acttgtattt cattttagtt tcagcaataa tatatcctat
     2461 tttcgttgaa ttagcatact tttgaacaaa aaataaatga cctttaaaag aatattcctt
     2521 tttatttggc tttctgtttt cttccatatt gcgcaagcac aaaaagtcac ttatgcacaa
     2581 caggaacttg atgcttattc ttatgattat ataaaaacag ccggatataa cgacaattct
     2641 ttttttgttt ttcagagcaa cttgcctttt gaaaacgatc gtgacagggt aggatttaaa
     2701 tcacgtaaat atagagttac ttgttttaac cggaatctct ctttaaaatg gtcgaaaaaa
     2761 atagagctta aaggtgaaaa agaaaaattg gaaaccatca cagtgattaa cggtcaggtt
     2821 gctttaatta aatcttcttt tgaaggtaaa gacaatcaaa tatccgttta tatcaccaca
     2881 ttagatgaca atggaaacga atcttcaacc aggaaagata ttggaactat tttgtttaac
     2941 aggacaagta accttgataa gataaaagtt attgcttcga aaggaaataa ctacttcggg
     3001 ctcattcaga atgaaaaaaa acaaaatggt tttcagcaac tgcattgcct gattattgat
     3061 agtgacttca aacttgttag gcagctgcaa tttgaagtac cctatcctga gaaaaatttc
     3121 aattacgaaa actggctttt gacagatgat gcagatttta ttcttgaagg cagctactac
     3181 tctaaagaaa aaagtgaaaa ccgcaaacgg tgggatgctt acaagttatt tgtatcttcc
     3241 aatggaaata gtggcgccat tgaatatgct atcaatacta gcaacctgat actaagcagt
     3301 agtgcaatag cttatgatgc aataaataaa aaattagttc ttgcagcctt ttatactgac
     3361 aatgtttcta caaaatcagg aataatttat gcaagccttg gaactgctgc aaatgattca
     3421 ctgcttatga taagacaacc cattggagat gctaccttaa cacgtatgtt tgccggtcaa
     3481 aatttagacc gtagcagcgc aggaattgaa aacctgagta tagaaaagct gatattaaga
     3541 aatgatggtg gcgctgtttt ggtaacagag gtttcttatg ttacagacta ctcctactac
     3601 gattatttta cgcaaaacta cattcgtaat tttgaatatc atttcaataa tgttattacc
     3661 ttttctatca atcctgatgg taccattgat tgggatgctg ttttgagaaa agagcaggtt
     3721 tcaacaaatg actacggaaa atattcttcg tttgccttag ccatgacaga aagtgacatc
     3781 agtttacttt ataacacgcg aacagacaga aacaatggtg ttgcctattt cggaataaag
     3841 agaaatggtg aaacagaatc gaaagaaatt atcaaacctg aagaacgaat tttattggtc
     3901 ccatttgctg caaaacaaat cagcactgac gaaattgttg ttccctgctg gaacaggaaa
     3961 aagatgctaa tggcaaaaat tactttctga tgcccggagc gttatctctt gcattacagt
     4021 atatttccta ttacgtcagg gcatggaaca aacataatgt tcattcgcct tttgtattca
     4081 aactcactac agaagttatt catcctgaca aaaaacttgc agcatttata cctattgaac
     4141 aacagagaaa acttttaaag caaaatgcgg ataaaattct tttcactgaa ttaggtgcag
     4201 gaaataaaag caatcaacga agtgtttcta acattgccgc aaaatcactg aaatctccaa
     4261 gatacgcacg tctactctat aggctgacaa atcattttca gcctgaaaat attattgagt
     4321 taggtacatc acttggtatt acaacaggct atctggcatt atccggctgt aaaaacatct
     4381 atactattga tggtaacaaa accgtatcag acattgcaaa agctacactt acctccgtta
     4441 aactgagcag caatgtaaaa tttataaatg gaaattttga tgagtctttg cctgcaacgc
     4501 taaacacttt aaacaaagtt gattttgttt tttttgatgg caatcaccgt tatcaaccaa
     4561 ccttaaatta ctttcaacaa tgcctgacaa aggctcatga caaaagtgtg tttgtatttg
     4621 atgatatcaa ttacagtcat gaaatgaaaa aagcctggaa agagatcaaa caacatccat
     4681 cagtagttgt tagtattgac ttgtttatga tgggaattgt tttttttgat acagaccttt
     4741 tgcgccaaca ttttgtaatc aggtattaac atttggtaac ttaattatca gccgcaattt
     4801 attactttta ccacgtacaa attaattatg ccacagtcca ttatttcgct ttcagatatt
     4861 tcacgacttt ataaagtcgg aacagaaaca atttatgcat tgcgatctgt ttcacttaac
     4921 atttttaaga atgaatatgt tgcattgatg ggtagttccg gttcagggaa atccaccttg
     4981 atgaatattc taggttgcct tgatacaccc tcaagcggta gctatcatct taacaacatc
     5041 tccgtttccg gaatgacaga taatgaactt gccgaggtaa gaaataaaca aatagggttt
     5101 atttttcaaa catttaatct gttgccacga agtacagcac ttgagaatgt agcattgccg
     5161 cttgtgtatg caggcgtttc aaaagaagaa cgtttaaaac gggccaaaga agtgcttgaa
     5221 agtgttggct tagccgatag gattatgcac aagccaaatg agttatccgg tggtcagcgt
     5281 cagcgtgtcg ctgttgcaag ggcattggtc aatcatccat ctatcattct tgctgatgag
     5341 ccaaccggaa atcttgattc aaaaacttct gaagaaatta tgttgctctt tgaagaaata
     5401 catcaacgcg gcaataccat tattgtagta acccatgaag aagacattgc ccgacatgct
     5461 caccgtattg tcagaataaa agacggcctc attgaagatg atgccattaa cacacaaata
     5521 acaaaagcaa aaattattgt atcatgaaca tcaaatcaat tcaatcgctg cttatagttg
     5581 tagtactatt agccagttgt ggggtagcca acagtcagaa aggtaaactc ctcacgcctg
     5641 aagtatttga aaaaatgctt cacgaaacac ctgaggctca agttatagat gtaagaacgc
     5701 ctgaagaatt taatgatggg catctgaaaa atgctttgaa catgaatgtc aacagcaaag
     5761 attttgaaaa ccgtgccccc tatctcgata aagcaaaacc tctttttgtt tattgctatt
     5821 ccggtggccg cagtgccaaa gcctgcgaat attttgagaa gatgggattc aaagtaattt
     5881 atgatatgga aggcggatat tctgcatgga cagatgcaaa cctgcctgta gaagggaaaa
     5941 aagctgataa ggcagggatt agtcttgata actttgcttt gcttacaagt ggtgcaaaag
     6001 ttttagttga tattaacgca ccttggtgtc caccatgtgt aaaaatgaaa cctatgttag
     6061 attcattaga cattgcttgg aaagacaaag taaaaattgt gcggttgaac aaagacgaaa
     6121 atcaattaat ttcaaaatcg cttcaggtaa acgaattacc tactttgctt ttttacgaaa
     6181 acggcaaatt ggtgaaacgt gaaagtggtt ataaaaatgc tgaacaacta aaagctatgg
     6241 ctcaggtgga atagttccta tgccaacgat atggcttcgt agtttcatta agtttaaata
     6301 aaacagttta tcactttcaa tactatttca ctcttttaca aaaaattaag aaagagaaaa
     6361 ccaataatta aaaaaaagcg gttggctttg ttggtgtctt tttctttggt tcgtttcttg
     6421 ggaaaatgaa cagaaaactc tatcacatcg tcaattttta atccgcctgt ggcggagggc
     6481 actacgtggc aggatagtgt ttgtgtgagt gtgaactacg catgtacaaa tagcagtact
     6541 atgatggcaa gcaacttatc tcattgattc atagtatata aaattaatat tttattagtc
     6601 ttgccttagc cttgacaata gtttaaattg tgccttttta gacatataaa acatgtaacc
     6661 ctaagggtat aaccacgtat aaagtcattt agttgttgag taaattatga atatatctgc
     6721 attagcagaa gccgaaaaac ttctatttat tgcaaggcag tcgctttcag aagggaaact
     6781 gcctcaagca atagatatgc tcaataaaat agttaccgat tttcctaatt tttcaaaagc
     6841 acatatcact ctggcagaca tttattttaa taaacttcaa gactcaacaa caggcgaaga
     6901 attttataaa aaagccattg ccattgccga tatgaatacg gatgccttta ccggttatgc
     6961 aagcttaatg ctgcaacaag aaaaatatcc cgaagcattg tcattgctca acaaagcgat
     7021 ggctataaaa ggtgcaccaa aagatactat ctacatgctg ctgggtaagc tctacgaaat
     7081 gcaaagcagg gttgatgatg ctatgaataa ttataaaaaa tgtattacct ccaccctatc
     7141 aaactccgtt cttgaagaag cagaacttgc attgaaaaga tgtgaggtca agaaaaagta
     7201 catttagttt ttcctttttc cggtgttgtg ttttatgctt tgctaataat tttattctgc
     7261 tttacaactg ataaaaatat ttcaaagtgc aatttcgaat gcggcaccta ttttcacaaa
     7321 tacattacat ttgcctttag actacaactg taaattaatg aaacgtgcct ttatcttttc
     7381 actgtgcttt attgtatctc attttataag ttttgctcag ttagaccaat atacttctga
     7441 acaaaataaa ttttattgga aaaatcgtaa accgacttta aactattggc aacaggatat
     7501 ttattataat attaaagctg taatagacga aaaagcagac attatcagtg gcattgaaga
     7561 acttacttac agaaacaatt ctcccgacac cttgaaattt atttttatgc atctctatca
     7621 gaatgcattt gttccgggaa gttatttgga cgacttaaac aaagccaatc aaaataaagt
     7681 gcgctttgga aaatatgaat cgcaagggtt gggaattgaa atcaccaatg taagtacact
     7741 atctgtcaat ggagtttcta taaataaaca agcagaaaaa acaattgaca ataccattat
     7801 gaaaatcatg ctgccagaac ctttgttgcc taatcatgat cttgttctta aaattgattt
     7861 taaaacat
//
LOCUS       DAOQNP010000102         7688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3568_length_7688_cov_7.482510, whole genome shotgun sequence.
ACCESSION   DAOQNP010000102 DAOQNP010000000
VERSION     DAOQNP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3568_length_7688_cov_7.482510"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1449
                     /locus_tag="PKY09_11350"
     CDS             139..1449
                     /locus_tag="PKY09_11350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNB13494.1"
                     /translation="MSQVELKMPKMGESVAEATITKWLKKEGDRVEQDEPILEIATDK
                     VDSEVPSTAAGILVKQLYNEGDVVAVGKSIAIIGDSASSAAPTPSVPKAEEVKIAVAP
                     VVATQSTTAATTAVSASSKFYSPLVRNIAQQENVSSAELDAIAGTGAEGRVTKKDILQ
                     FIENRKSGNGQIAQNTSAPAVQPATTPQPKAPAAIPVTDGDVEIIEMDRMRKLIADHM
                     VMSKHTSPHVTSFVEADVTNMVLWRNKVKNDFEKREKEKITFTPLFIEAIVRAIKDYP
                     MINCSLDGTKILLKKNINIGMATALPSGNLIVPVIKNADRMNLVGLTKSVNDLSGRAR
                     KNKLSPDEIAGGTFTLTNVGTFGNVMGTPIINQPQVAIMAAGAIRKKPAVIETEFGDT
                     IGIRHMMFLSLSYDHRIVDGSLGGSFLRKVADYLEQFDVNRNLL"
     gene            1456..3054
                     /locus_tag="PKY09_11355"
     CDS             1456..3054
                     /locus_tag="PKY09_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13495.1"
                     /translation="MRFLFVIIPLLWLNVAVAQQPKKPVKKVVLGKDQVSEMKKAGAE
                     YFKSDNYKSALPIYKQLQENDPNNTEFNYRLGQCYLNTNVNKKLAAGFLIKAAEGKDV
                     PKDVYYYTGRALLILEEIDDAVDAYEKFKIAHGGKVNAKLNLENNVEWCYHFRDMKKT
                     PIQVSFTNLGKLVNSPNNDYRPVCDMFGNNVYFSSNRKGNIGAIVDGYGEIISDGYFT
                     KKDTGYSKAKTLGTGINTMYYEEPLQLNANGDKLLISKEGGSISSPLYTSEINGKSFG
                     KAVAINKFPASKVDGACMTDDGNTIYFSADLKGGKGGKDIWMTTLNSKNEWSDPVNLG
                     DAVNTKYDEINPWLFFDNQTLFFASEGHNSIGGFDIFFSTKPHESMDWSTAKNVGYPL
                     NTTDDDKYFSLTADCKTGFVSQTTEHGSGELDIIKFVCANPIIKQEKVVCDFTFLKAD
                     GTFARDANCMIIRNDTGESSGVKTINGSNGKLYIYLSPGNYKVKVKGVKSGRADEDFI
                     ITGNEPKMQLVKTFKLTPPVKEGK"
     gene            3056..3826
                     /locus_tag="PKY09_11360"
     CDS             3056..3826
                     /locus_tag="PKY09_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNB13496.1"
                     /translation="MNLKLHRPLAFFDLETTGVVIGMDRIVELGILKILPDGTKTNKT
                     WRINPEMHIPEVVSKIHGIYDKDVADCPTFKEIANDVNHYLNNVDLAGYNSNRFDVPL
                     LVDEFLRAGIEFDIRGRKLIDVQHIFHKMEQRTLSAAYKFYCNQELIDAHSAEADVRA
                     TYEVLEAQLERYSDQLQPDAAFLHSFCNNSKNVDLAGRIVYDDKKREVFNFGKYKGRL
                     VTDVFKTDPSYYDWMLKGDFSRDTKQVVTALRLREREN"
     gene            3905..6160
                     /locus_tag="PKY09_11365"
     CDS             3905..6160
                     /locus_tag="PKY09_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNB13497.1"
                     /translation="MGSENNSNKNNLPAVSASGKDSKVVIDLEAEKKEISKRYRHLLR
                     ACKSDLDKKDKKIIRLAFDTALEAHKEMRRKSGEPYIYHPIAVAQIVAEEIGLRSAIP
                     VVCALLHDTVEDTYLTLDDIRDLFGPVAEQIIDGLTKIAGVFDHSSSLQAENFRKMLL
                     TLSVDVRVILIKLADRLHNMRTLGSMARDKQLKIASETAYLYAPLAHRLGLYNVKTEL
                     DDLALKYTEPDIYITIAEKLRDTKKERDKFISEFIHPIKDELNKQGFKFEIKGRPKSI
                     HSIWEKMKKQGVTFEEIYDLFAIRIIIDSPQSQEKADCWKVYSIVTDSYRPNPDRLRD
                     WISTPKSNGYEALHTTVMSDQGKWVEVQIRSQRMNETAEKGFAAHWKYKESSQESALD
                     EWLTRIREMLENPDSNALDFVDDFKLNLFADEIFVFTPKGDLKTLPVKSTALDFAYEI
                     HTDIGSRCIGAKVNHKLAPISHTLNSGDQVEILTSDKQRPKEDWLSFVITAKAKGRIK
                     SALKDEKRILGEQGKEIFDRKIKHLKIPVAEINMNDILLYYKLPNVLELYYRIAMDAI
                     DLKQLREAVNYKDQRVRSQRTAEPTLEELVKQARGSTDMLVIGENLNKIDYKLSPCCN
                     PIPGDDVFGFITINDGIKIHRVNCPNAMQLMSNFAYRIVKAKWTGQEQLSVLAGIKIT
                     GMDDVGVVNNITKVISSELKVNMRSISIESNEGLFEGTIMLFVHDTEHLTKLMKKLSA
                     LSGILNVTRIT"
     gene            6164..6664
                     /locus_tag="PKY09_11370"
     CDS             6164..6664
                     /locus_tag="PKY09_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13498.1"
                     /translation="MYVPFDQLPDDSRIWIFQSDRLLSEEDIEAINPILNYFISDWTA
                     HQQTLRASFTFLHNHFLIIGVDENMAVASGCSLDKVFHVVRACGQKINTDFLNRLRIA
                     EMTHHTITLHTVKQILEIAENRNSNEPLMIFSNIIETKSEMKNNWLIPIEKSWLAPKL
                     KSASKI"
     gene            6682..7032
                     /locus_tag="PKY09_11375"
     CDS             6682..7032
                     /locus_tag="PKY09_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNB13499.1"
                     /translation="MNFLIKLIISTLAVMMVSYILPGVHVQSFGIALMVACVLAFLNA
                     LIKPILVLFTLPITLITLGLFLLVINAIIIIITDRLIDGFEVSSFGIALLFSLILSIV
                     TSVMESLAGQNQRQ"
     gene            complement(7084..>7688)
                     /locus_tag="PKY09_11380"
     CDS             complement(7084..>7688)
                     /locus_tag="PKY09_11380"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNB13500.1"
                     /translation="SGSGDGNMADNIEIANSFLSAGYNVAMYDYRGYGSSSDFKIDPD
                     LFIYPQFIVDLNAVCDYLRKSRAITKFNLYGKAIGAGLSIGVGANRTETQKVIADGPW
                     IGLEVMKKKIKDKTGKEVVMPFGFDKNHEPMYAMEKPKGIKALMVIVSSQDNLINPTD
                     VKQIKGISETYVVKNSPSNAENFTTDKNAYFEKISKFLGN"
BASE COUNT         2626 a         1354 c         1448 g         2260 t
ORIGIN      
        1 aagtattctc aaaaaatctg ctaaactttt acaaaactta tttagcgaat ttaaatgtca
       61 ataaaatata aagaacaacc ctgcataatt taattaaaac tgtaactttg agcaataaaa
      121 ttttgcacgt aaaatactat gtcacaagtt gaactgaaga tgcctaaaat gggcgaaagc
      181 gttgctgaag caacaattac caaatggtta aaaaaagaag gagatcgcgt tgaacaggat
      241 gagccaattc ttgaaattgc tacagataaa gttgactctg aagtgccttc cacagcagca
      301 ggaatattag taaaacaatt atataatgaa ggtgatgtag ttgccgttgg aaaatctatt
      361 gctatcattg gtgattctgc atcatctgca gcaccaactc cttcagtacc taaggcagaa
      421 gaagttaaaa ttgccgttgc acctgtagtt gcaacacaat ctactaccgc tgctactact
      481 gcagtaagtg catcttcaaa attctattca ccattagtta gaaatatcgc acaacaggaa
      541 aatgtttctt cagcagagct agatgcaatt gccggaaccg gtgcagaagg tcgtgttaca
      601 aagaaagata ttcttcaatt tattgagaac agaaagtccg gtaatggtca gatagcacaa
      661 aatacatcag cacctgctgt acaacctgca acaacaccac aacctaaagc acctgcggct
      721 atacctgtga ctgatggaga tgtagagatt attgaaatgg atagaatgcg gaagttgatt
      781 gcagaccaca tggtcatgtc aaaacacacc agtccgcatg ttacatcatt tgtagaagcc
      841 gatgttacca atatggtatt gtggcgtaat aaagttaaaa atgattttga gaaaagagag
      901 aaagaaaaaa tcactttcac tccattgttt atagaagcaa ttgtacgtgc aataaaagat
      961 tatccaatga ttaactgttc acttgacggc actaaaatac ttctcaagaa aaacattaat
     1021 attggtatgg caactgcatt acccagtggc aaccttattg tgcctgtgat aaaaaatgct
     1081 gacagaatga accttgttgg tcttacaaaa tcagtaaatg atttgagtgg cagagcgcgc
     1141 aaaaataaac tttcacctga tgaaattgca ggtggaactt ttactttgac caatgtaggt
     1201 acgtttggaa atgttatggg tacacccatt atcaatcaac cacaagtggc cattatggca
     1261 gcaggagcca ttcgtaaaaa acctgctgta attgaaacag aatttggtga cactatcggt
     1321 attcgtcaca tgatgtttct ctcattgtct tacgaccatc gtatcgttga tggaagcctt
     1381 ggtggtagtt ttcttcgaaa agtagccgac tatcttgagc agtttgatgt taacagaaac
     1441 ttattataaa ttattatgag atttttattt gtcattatcc cattactttg gctgaatgtt
     1501 gcagttgcac agcaacctaa aaagccggtt aaaaaagttg ttttaggaaa agatcaggtt
     1561 tctgaaatga aaaaagcagg tgcagaatat tttaaaagtg ataactataa gtcagcattg
     1621 cccatctaca aacaacttca ggaaaatgat cctaacaata ctgagtttaa ttacaggctt
     1681 ggccaatgtt atttaaacac caatgtaaac aaaaaattag cagccggttt tctgattaag
     1741 gctgctgaag gaaaagacgt tccaaaagac gtttattatt ataccggtcg tgcgcttctt
     1801 attcttgaag aaattgatga tgcagtagat gcctatgaga aatttaaaat agctcatggc
     1861 ggaaaagtta atgcaaaatt gaatcttgaa aataatgtgg aatggtgtta tcactttcgt
     1921 gatatgaaga aaaccccaat acaagtaagc tttactaatt tgggtaaatt agttaattca
     1981 ccaaacaatg attacagacc tgtttgtgat atgtttggca acaatgttta cttcagttcc
     2041 aatagaaaag gtaatatcgg agcaatagtt gatggatatg gtgaaataat ttcagatggt
     2101 tattttacaa aaaaggatac gggttatagt aaagctaaaa cacttggtac aggtatcaat
     2161 actatgtatt atgaagaacc tttgcaatta aatgcaaatg gtgataaatt attgatttct
     2221 aaagaagggg gcagtatttc ttctccatta tatacgtcag aaatcaatgg aaaatcattc
     2281 gggaaagcag ttgcaataaa taaatttccg gcttcaaaag ttgatggtgc ctgcatgaca
     2341 gatgatggaa atacgattta tttttctgca gaccttaaag gtggcaaagg cggaaaagac
     2401 atttggatga caactttaaa cagtaaaaac gaatggtcag atccggtgaa tcttggtgat
     2461 gcagtaaata caaaatacga tgaaataaat ccttggttgt tttttgataa tcagacttta
     2521 ttttttgcat ctgaaggtca caacagcatt ggtggatttg atattttttt cagtactaaa
     2581 cctcatgaat ctatggattg gagtactgct aaaaatgtag gctatccatt aaatactaca
     2641 gacgatgata aatatttttc tttaacagcc gattgtaaaa cagggtttgt aagtcagact
     2701 acagaacatg gttcaggaga gttagatatt ataaagtttg tttgcgccaa ccctatcatt
     2761 aaacaagaaa aagttgtttg tgattttact tttttaaaag ctgatggcac ttttgcgcgt
     2821 gatgcaaatt gtatgatcat cagaaacgat accggtgaat catcaggagt gaaaacaata
     2881 aacggctcaa acggtaaact ctatatttat ctcagcccgg gtaattataa ggttaaagtt
     2941 aaaggtgtaa aatccggtcg tgctgatgaa gattttatca tcacaggtaa cgaacctaaa
     3001 atgcaattgg taaagacttt taaactgaca cctcccgtta aagaaggaaa ataaaatgaa
     3061 tctgaaacta cacagaccac ttgccttttt tgatcttgaa actacaggag tagttatcgg
     3121 catggacaga attgttgaat tgggtatttt aaaaattctc cccgatggaa ctaaaaccaa
     3181 caaaacctgg cgtatcaatc ctgaaatgca cattcctgaa gtagtctcaa aaattcatgg
     3241 catttatgat aaagatgttg cagactgccc tactttcaaa gaaatagcaa acgatgtaaa
     3301 tcattatctc aacaatgttg acctggccgg atacaattca aaccggtttg atgtaccctt
     3361 attagttgat gaatttttaa gagccggtat tgaatttgac atacgtggac gcaagttaat
     3421 tgatgtgcag cacatctttc acaaaatgga acagcgcacc ttgagtgctg catataaatt
     3481 ctactgcaat caggagttaa ttgatgcaca ttctgcagaa gccgatgtca gagcaaccta
     3541 tgaagttctg gaagcacaac tggagcgtta tagcgatcag ctacaacctg atgctgcatt
     3601 ccttcactca ttttgcaaca acagtaaaaa tgttgatttg gccggacgta tagtttatga
     3661 tgataaaaag cgcgaagtat tcaattttgg aaagtataaa ggccgcttgg taacagatgt
     3721 ttttaaaaca gatccgagct attatgactg gatgcttaag ggtgatttct caagagatac
     3781 caaacaagtt gtaacggcat tacgtttacg cgaaagagaa aactaaatat atgtttgacc
     3841 tgtttgatta attctactga caaaaaaaat tgcaggtaac ataaaaatct gtacttttat
     3901 actgatgggt tccgaaaata attcaaataa aaataatctc cctgctgttt ctgcttcagg
     3961 caaagacagc aaagtagtta ttgaccttga agccgagaaa aaagaaatca gtaaacgcta
     4021 tcggcattta ttgagggcat gcaaatccga tctcgataaa aaagataaaa aaataatcag
     4081 gcttgcattt gatactgcat tggaagcgca taaagaaatg cgaagaaaat ccggtgaacc
     4141 atatatctat catccaattg ccgttgcaca gattgttgca gaagaaatcg gtcttcgttc
     4201 tgccatccct gtagtttgcg cattgttgca tgacactgtt gaagatacct atcttacatt
     4261 agacgacatc agagatttgt ttggccctgt tgcagaacaa attattgatg gactgacaaa
     4321 aattgcaggt gtttttgatc attcaagttc attacaggcc gagaatttca gaaagatgct
     4381 tcttacattg agcgttgatg taagagtaat tctcatcaaa cttgccgacc ggcttcataa
     4441 catgcgcacg cttggaagta tggcacgtga caagcaactg aagatagctt ctgaaactgc
     4501 ttacctctac gcacctttgg cacatcgtct tgggctttat aatgtaaaaa cagaacttga
     4561 tgatctggct ttgaaatata cagaacctga tatatatata accattgctg aaaaactaag
     4621 agacactaaa aaagaacgtg ataaatttat ctctgaattt attcatccga taaaagatga
     4681 gttgaataag caaggattta aatttgagat taaaggaagg ccaaaatcca ttcactccat
     4741 atgggagaaa atgaaaaaac aaggtgtaac ttttgaagaa atatatgatc tatttgcaat
     4801 aagaataatt attgattcac ctcaaagtca ggaaaaagcc gattgctgga aagtatattc
     4861 tattgttacg gatagttacc gtccaaaccc tgacagacta cgtgattgga tttcaacacc
     4921 aaaatcgaat ggctacgagg cactacacac cacagtgatg agtgatcagg gtaaatgggt
     4981 tgaagtacag attcgttcac aaagaatgaa tgaaactgct gaaaaaggtt ttgctgcaca
     5041 ttggaaatat aaagaatctt cgcaagagtc tgctttggat gaatggctta cccgcataag
     5101 ggaaatgctt gaaaacccag atagcaatgc actcgatttt gtagatgact tcaaactcaa
     5161 tctctttgca gatgaaattt ttgtttttac tcctaaagga gatttaaaga cattgcctgt
     5221 caaaagcact gcattagatt ttgcttatga gatccacaca gatattggca gcagatgtat
     5281 aggagctaaa gtaaaccaca agttagctcc catttcgcat acactgaata gtggtgatca
     5341 ggtagaaatt ttaacctccg acaaacaacg tccaaaagaa gactggctga gttttgtgat
     5401 tactgcaaaa gctaaaggca gaattaaatc agcattaaag gatgaaaaaa gaattcttgg
     5461 tgaacaagga aaagaaattt ttgaccgaaa aataaaacac cttaaaattc ctgtagcaga
     5521 aatcaacatg aacgacattc tgctttatta caaacttccg aatgttttag agttatatta
     5581 tcgcatagca atggatgcaa ttgatttgaa gcagttgcgt gaagccgtaa actataaaga
     5641 ccagcgtgtg cgcagtcagc ggacagcaga gcctacttta gaggagttgg taaaacaagc
     5701 acgcggctca accgatatgc ttgtcattgg tgaaaacctg aataaaattg attataaact
     5761 ttcgccatgc tgcaacccta ttcccggtga tgacgttttt ggtttcatca ccatcaatga
     5821 cggaataaaa atacatcgtg tcaactgccc caatgccatg cagctgatga gcaactttgc
     5881 ttacagaatt gtaaaagcta aatggacagg tcaggagcag ctttctgtct tggccggtat
     5941 taaaataaca ggaatggatg atgttggggt tgtaaacaat attaccaagg ttatctcaag
     6001 cgaattaaaa gtgaacatgc gatctatctc tatcgaaagt aatgaaggtt tgtttgaagg
     6061 aaccatcatg ctttttgttc acgacacaga acatctgacc aaactgatga aaaaactttc
     6121 cgcacttagc ggtattctga atgtaactcg aattacttaa attatgtacg taccttttga
     6181 ccaattaccg gacgactccc gcatctggat ttttcagtcg gatagattat tatcagaaga
     6241 ggatattgaa gctatcaatc caattctgaa ttattttatt agtgactgga cagcacatca
     6301 acaaacttta agagcctcat tcactttttt acataatcat tttctgatca ttggtgttga
     6361 tgaaaatatg gcagttgcca gcggatgttc tctcgataag gtttttcatg tagttcgtgc
     6421 atgtggtcaa aaaatcaata ctgacttttt aaatcgtctt cgcattgctg aaatgactca
     6481 tcatacgata acacttcaca ctgttaaaca aatattggaa attgcagaaa acagaaacag
     6541 caatgagcca ttaatgatat tcagtaacat cattgaaaca aaaagtgaaa tgaaaaacaa
     6601 ttggcttata ccgattgaaa aatcatggct tgcacccaaa ctaaaatcag ctagtaaaat
     6661 ttaaattaat aaaatcaatt tatgaacttt ttaattaaac tcataatctc tacgctggca
     6721 gtaatgatgg tgtcatacat tctgcccggt gtgcatgttc agagttttgg cattgcactt
     6781 atggtagcct gtgttttagc atttttaaat gcattgataa aaccaatact tgtacttttc
     6841 acactgccaa taacactcat cacattaggt ttatttttat tggtcatcaa tgccatcata
     6901 attatcatca cagaccgatt gattgatgga tttgaagtaa gcagttttgg cattgccttg
     6961 ctgttctctt tgatattaag catcgttact tcggtaatgg agtcgcttgc cggacaaaat
     7021 caaagacaat aaaaaaaccg gcaacttttt aaactgccgg tctttgtgct gtcgaacaaa
     7081 caatcagttt cctaaaaatt tagagatttt ttcaaaataa gcatttttat cggttgtaaa
     7141 attctctgca ttggatggcg agttttttac aacataagtt tcgctgatgc ctttaatttg
     7201 ttttacatct gttgggttaa tcagattatc ctgcgatgaa acaataacca taagtgcttt
     7261 tattccttta ggcttttcca ttgcatacat gggctcatga ttcttatcaa atccaaaagg
     7321 cattaccacc tctttgcctg tcttatcttt tatttttttc ttcattactt caagaccaat
     7381 ccaagggcca tcggcaatca ccttctgtgt ttctgttctg tttgcaccaa caccaattga
     7441 aaggcctgcg ccaatagcct taccatacaa attaaatttt gtaatggcac gcgacttacg
     7501 caaataatca caaacagcat taaggtcaac aataaactgc ggatagataa ataaatccgg
     7561 atctatttta aaatcactgc tgctaccata accgcgatag tcatacatgg caacattata
     7621 tcctgcactt aaaaatgaat tggctatttc aatattatca gccatattgc catcaccact
     7681 accactca
//
LOCUS       DAOQNP010000103         7570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3655_length_7570_cov_9.721756, whole genome shotgun sequence.
ACCESSION   DAOQNP010000103 DAOQNP010000000
VERSION     DAOQNP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3655_length_7570_cov_9.721756"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="PKY09_11385"
     CDS             complement(<1..1622)
                     /locus_tag="PKY09_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNB13501.1"
                     /translation="MPSKAIQKVTRLFWLFFLIAISAFAQKSDVFRHLNTVNGLSHNR
                     ITCIIQDKIGFIWIGTEDGLNRYNGYKIDVYKHNPSDETSVSSNGIRCLFEDSKGKLW
                     IGTDDGLSFFDIETEHFASFRTNSKGNSISSNQITCMEEDASGVLWIGTAGGGINLFN
                     PSTGKWQNFVVNKSDPNSICSNNITAIVKDKNGVMYLGTDNGLAIADRNQAGNFTIID
                     HDPANTNSPLENSITSLYADFQNKIWIGTLNSGMSAMRTDNRSFTHYTDLGKFASNSV
                     FGINKDLDGSIIAGTIGGGLIIFNSDASTYSSFTHSKTDPFSISSNSIWTVYRDKAGI
                     IWIGTDNGLDYYKEDLMRFKTELPLPDDPLSNSIPNKNVFCFLPEGNNLWMGVLGTGV
                     VVKESTGTIKQIYSTANGLADNSVFCLIKDRNGIVWVGTYNGLTSINPQSGVLRSYRI
                     ADGLTNSNIRSLAEDENGNIYIGTYGGGLCVLNRQTDKISSIRKEDGKGLTNNIITAL
                     KYDGNGNLFIGTYGGGLCLLNIAANSITAFES"
     gene            complement(1640..2842)
                     /locus_tag="PKY09_11390"
     CDS             complement(1640..2842)
                     /locus_tag="PKY09_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13502.1"
                     /translation="MEMLRSLLYNILFMLMPLTVLSQSDTLSRSTNIVGCQSGDCING
                     TGVYLYKNGNRYEGAFKNGSPDGKGVMQYRNGNRYKGDFTQGRLNGKGNYSYRNGNSY
                     TGDFVNDQFHGRGIYIYNSGNQYIGDFFEGSKHGSGTFTFNDGSTYTGQFVDDMVQGK
                     GTWRYANGDWYEGDFVNSKKSGKGTYYYNDSSRYSGDFVNDVYNGSGVFTFADGSTYI
                     GQFRNDLFNGQGTCYYSNGDVYTGEFRDGLYSGSGVYAYATGDRYKGTFLNNVFNGEG
                     TYIDESGEETVTMYENGRAVGESKVIDKENVKPIPEESKNETKKPEEKKEEPKTSEPT
                     VAKIPCPACNGKGRVLQPEVKRNRSVVKDISNGLGPRNFVTYTVSEVVKPAAWATCKV
                     CHGSGIKQ"
     gene            complement(2814..4979)
                     /locus_tag="PKY09_11395"
     CDS             complement(2814..4979)
                     /locus_tag="PKY09_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB13503.1"
                     /translation="MKKQILVFIFCLICTLVNGDAKHFYSVFDRNDLPVFSRPFFATL
                     TADPVALKSAERMLDDAMKSNSQHEIYQNANNAGYYNLLNGDFLQALKFLQIAVNHKP
                     QGKEAGTIMAQYAFALDVAGRHEQAFTIYNELLEKELANATQIKAYVQALAGLIFLQH
                     ADTVSTKPLLSKALQYFSANNFLLQQYYLYERMGDINLISKNFNSASANYLQQLKYAA
                     KLNNRTLQALATRNLGLCYFKRDDYLKAASYFKQSLDFRNNVLLQKLLKDTYLKIVTI
                     SSFNNDFESADHYHELYRQLKANETVVRETAALQAEKEKILFLLSKSVNNHNNMVSQQ
                     EYELSQQLTTLEIERQNKEKALEELTLAEAQKKLKELELDKIAGEKIKQEAELAKKEL
                     QISRQKEFRNILLGISGLIVLGIIFLINRYRLKRKSLEQLRQAHQELRQAHEQLKQTQ
                     QQLIQSEKMASLGQLTAGIAHEIQNPLNFVNNFSTLSMDMIEDYLKNKDETLLEELKS
                     NLKRINHHGGRVSSIVKGMLLHSRNKTTEKELSDINLLVDESLALAFHGKKSTEADFY
                     CHIEKNYDVTAPAIKVFPQELRRVIINLVNNAFYAVHERATKSNANKDETKYKPQVSV
                     ATHVVNQFLEITITDNGTGIPDAIKDKIYNPFFTSKPTGKGTGLGLSVSFDIIEKQHG
                     GKLKFESEQGVGTVFTISLPLIAPHDLKQNNGDVKKSVV"
     gene            complement(5039..7495)
                     /locus_tag="PKY09_11400"
     CDS             complement(5039..7495)
                     /locus_tag="PKY09_11400"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNB13504.1"
                     /translation="MRKFFCLILLGNIFSSAVFSQGIVSGVVKDKNHKPVAYANVYVK
                     PGFVGTTTSNDGIFEIELAPGSYTLYCQNLGYKQFSKQIEVGAGKQQIEIELTEELYS
                     LKEVAVTNNKEDPAYEYIRKAIAKRKSYLNEVDAYSCNVYIKGVQKLNKYPEKFLGKV
                     VDFGGMVDSTTGIFYLSESVSKYSYEKPGKIREELVSSRFSGNNKAFSYNKASDMQFN
                     FYENRVMSEGISSKGFVSPVAGDALFYYRYKLLGSVVENDRLIHKIEVTPRRQNDPVF
                     RGVIYIADDLWRFQFIDLFLTREAGIEFLDTLRIKQSFVPVENNVWMPVTNLFDFSFR
                     ILGFQGGGNYVGYFSDFKINPVFPAKTFSAEILKISPDANKKDSAYWDTVRPIPLTQD
                     ETSDYFKKDSLATIRESKQYKDSMDRINNKFSVFNFLLAGYSYRHSYREMTYTIKPIA
                     NTFSFNTVEGWNIALNTEFVKRLPNRKRLTVFTNTRYGFSNTHWNANAGFNYLFNRFN
                     DNTISFEGGTAVNELNSTNPVKPLVNMLYTLWAERNYLKIYEKQFLKVSYGKEWFNGF
                     NATAEIEYGRRNALTNTSDYTWKNFKNRTYTSNNPISPDYDIKIFLPHHYFNTHLTLT
                     YSPFTKFITRPDGKVREESGWPLLFMHVNKAFGNVINSKTDYLSFDAGFKHQIDFGML
                     GFVVYNCRYYRFVNRKHVEFPDFFHFGGNKTIVSDFGTEDFAYLDYYKYSNTRDAFAI
                     HAEYNLNRFITNKIPLIRKLKLNEIVGFHFLKVNEIKSHYELTVGLEKLGIIRADFVC
                     SYDGKARPFYSFRFGFKLRQ"
BASE COUNT         2327 a         1519 c         1159 g         2565 t
ORIGIN      
        1 tttgattcaa atgcagttat ggaatttgca gcaatattta acaaacacaa tccgccacca
       61 taggtgccga taaataaatt tccattacca tcatatttta aagccgtaat gatattgttc
      121 gttaaacctt taccatcttc cttacggata cttgatatct tatcagtttg tctgttaagt
      181 acgcaaagcc cacctccgta ggtaccaata taaatattcc cattttcatc ttcagcaagc
      241 gatctgatat tactattggt taatccgtca gcaatcctat aagaccgcaa aactcctgac
      301 tgtggattaa tagaagttaa gccattatag gttccaaccc aaacaattcc atttctatct
      361 ttaatcagac agaacacact gttgtctgca agtccatttg ccgtactata gatctgtttg
      421 attgtgcctg ttgattcttt aactaccaca cctgttccta aaacacccat ccataaatta
      481 ttcccttcag gcaaaaagca aaaaacattt ttattaggga ttgaattact caacgggtca
      541 tcaggcagtg gcaattctgt tttaaaacgc atcagatctt ctttgtaata atcaagacca
      601 ttatctgttc caatccagat tatgcctgcc ttatcccgat agactgtcca aatactattg
      661 ctgctgatgg aaaaaggatc tgtcttacta tgggtaaatg aagaataagt tgaagcatcg
      721 ctgttaaaaa ttatcaatcc tcctcctatt gttccggcaa taatacttcc atcaagatct
      781 ttatttattc caaatacaga gttgcttgca aacttgccca aatcggtgta atgagtaaaa
      841 gaacgattgt cggttcgcat agcactcata ccactgttga gcgttcctat ccaaatctta
      901 ttctgaaaat cggcatatag gcttgtaatg ctgttctcta aaggagagtt tgtatttgca
      961 gggtcatgat caatgatagt aaagttgcct gcctgattcc ggtcggcaat ggcaagacca
     1021 ttgtctgtac ctaaatacat taccccattc ttatctttta caatagccgt gatattatta
     1081 ctacaaatag aattaggatc tgatttgttg acaacaaaat tctgccattt tccggttgaa
     1141 ggattaaaaa gattaatacc gccaccggca gttccaatcc aaagaactcc ggaggcatct
     1201 tcttccatgc aggtaatctg attgctgctg atggaattcc cttttgaatt tgttctgaaa
     1261 cttgcgaaat gttcggtttc aatatcaaaa aagcttaatc catcatcggt gcctatccat
     1321 aattttcctt tactatcttc aaacaaacaa cgaataccgt tgctggatac tgaagtttca
     1381 tctgatgggt tgtgcttata aacatcaatt ttatagccgt tataacggtt cagtccatct
     1441 tcagtaccta tccagataaa accaatttta tcctgaatga tacaagtaat tctgttatga
     1501 gaaagtccgt ttacagtatt gagatgcctg aacacatcac tcttttgtgc aaaagcacta
     1561 atggctatca gaaaaaataa ccaaaaaagg cgggtgactt tttgaattgc ttttgaaggc
     1621 atgggttcaa aagtaagatt tattgtttaa ttcccgaacc atgacaaact ttacatgttg
     1681 cccatgctgc aggttttact acttcagaca ctgtgtaggt aacaaaattt ctggggccta
     1741 aaccattact gatatcttta actacagagc ggtttcgctt aacctcaggc tgtaaaactc
     1801 ttcctttacc attacaggca gggcaaggaa ttttagcaac tgttggctca gaagtcttag
     1861 gctcttcttt tttctcttca ggtttctttg tctcattttt actttcttcc ggaatgggtt
     1921 ttacattttc cttgtcaatt actttcgatt ctcctactgc cctgccattt tcatacattg
     1981 taacagtttc ttcacccgac tcatcaatat aagttccttc accattaaaa acgttattca
     2041 aaaaagttcc tttgtaacga tcaccggtgg cgtaggcata aacaccactt ccactgtaaa
     2101 gtccatctct gaattcaccg gtataaacat caccgttgga ataataacat gtaccctggc
     2161 cattaaacaa atcatttcta aactgaccta tataagtact tccatcggca aatgtgaaaa
     2221 cacctgagcc attataaaca tcattaacaa aatcgccact ataacgacta ctgtcattat
     2281 aataataagt gcctttacct gatttttttg aattgacgaa atcaccttca taccaatcgc
     2341 catttgcata tctccaggtg cctttacctt gtaccatgtc atcaacaaac tgtcctgtat
     2401 aagtacttcc atcattgaag gtaaatgttc cactgccatg cttgcttccc tcaaaaaaat
     2461 cgccaatata ttggttgccg ctgttataaa tataaatgcc acggccatga aactgatcgt
     2521 ttacaaaatc acctgtatag ctgtttccat ttctgtaact gtagttacct ttaccattta
     2581 gtctgccttg tgtaaaatct cctttatagc gatttccgtt tctgtattgc attacacctt
     2641 taccatcagg gctaccattt ttaaaagcac cttcataacg attaccattc ttatacaagt
     2701 aaacgcctgt gccattgatg cagtcaccac tttggcaacc tacaatattc gttgaacgac
     2761 ttaatgtgtc tgactgtgac aaaacagtca acggcattaa catgaacaat atattataca
     2821 acagacttct taacatctcc attattttgt tttagatcat gtggtgctat cagcggcaaa
     2881 gaaattgtaa aaactgttcc aacaccttgt tctgactcaa attttaactt tcctccatgt
     2941 tgtttttcaa ttatgtcgaa gcttactgac aaaccgagac ccgtgccctt accggttggc
     3001 tttgatgtaa aaaacggatt ataaatttta tccttgatgg catcaggtat gcctgttcca
     3061 ttatcagtaa ttgtaatttc cagaaactga tttacaacat gtgttgcaac actcacttgc
     3121 ggtttatatt ttgtttcatc tttattggca tttgattttg ttgcccgctc atgcactgca
     3181 taaaatgcat tattgacaag attgataatc actctacgta actcctgagg aaaaacttta
     3241 attgcaggtg ctgtaacatc ataatttttt tcaatatggc aatagaaatc tgcttctgtt
     3301 gatttcttgc catgaaaagc taatgccagc gattcatcta caagaaggtt aatatctgac
     3361 aactctttct ctgttgtttt gtttcgacta tgcaacagca tacctttaac aattgatgaa
     3421 actcttccac catgatgatt aattcgttta agatttgatt tcaattcctc aagtaaagtt
     3481 tcatctttat ttttcagata atcttcaatc atatccatac ttaaagtaga aaagttattt
     3541 acaaaattca atgggttttg tatttcgtga gcaattcctg ccgttaactg acctaaagaa
     3601 gccatctttt cagactgaat aagttgttgc tgcgtttgtt ttaactgctc atgtgcctgt
     3661 cgcaattcct gatgagcctg tctgagttgt tctaatgact ttctttttag tctatagcgg
     3721 tttatcaaaa atattattcc caaaactatc agtccggaaa tgcctaatag aatatttcta
     3781 aattcctttt gcctgctgat ttgcaattct ttctttgcaa gctcggcttc ttgttttatt
     3841 ttttctccgg caattttatc cagctcaagc tccttcaact tcttttgtgc ttcggcaaga
     3901 gtcaattcct ctaaagcttt ttctttattc tgacgttcga tttcgagcgt agttaattgc
     3961 tgactaagtt catactcctg ctgcgaaacc atattgttat ggttattgac agacttactt
     4021 agcaaaaaca gaattttttc tttttctgcc tgcaaagctg ctgtttctct aacaactgtt
     4081 tcattagctt ttaactgtct atataactca tggtagtgat cagcgctttc gaaatcatta
     4141 ttaaaactac tgattgttac aattttaaga tacgtgtctt ttaacaattt ctgtaaaagg
     4201 acattgtttc taaaatccag gctttgttta aaataagatg cggcctttaa ataatcatca
     4261 cgtttgaaat aacataaacc caaattacga gtagccaatg cctgcaaagt tctattgttc
     4321 aacttggcag catatttcaa ttgttgcaaa taattagcag atgcactatt gaaatttttg
     4381 gaaataagat ttatatcgcc cattcgttca tacaggtaat attgctgcaa caaaaaatta
     4441 ttggctgaaa aatattgtaa tgccttactg agtaatggct ttgtcgaaac ggtgtctgca
     4501 tgctgaagaa atatcaatcc tgccaatgcc tgtacatatg cttttatttg tgttgcgttg
     4561 gctaattctt tttcaagcaa ttcattataa atagtaaatg cctgttcatg cctaccggca
     4621 acatcaagtg caaatgcata ttgcgccatt atagttcctg cttccttacc ttgcggttta
     4681 tgatttactg ctatttgcaa aaattttaat gcctgaagaa agtcaccatt cagcagatta
     4741 taatatcctg cattattggc attttggtaa atttcatgtt gactgttact cttcattgca
     4801 tcgtctaaca tcctctccgc agactttaaa gcaacaggat ctgcagttaa agttgcaaaa
     4861 aatggtcttg aaaaaacagg taaatcgttt cggtcaaaga cagaataaaa atgctttgca
     4921 tcaccattaa ccaatgtgca aataaggcaa aagataaaaa ctaaaatttg tttcttcatc
     4981 accgagacga atgccaaatg tattcaattt ttatcaattg acgatgtatc ttttaatctt
     5041 actgccttag cttgaatcca aaccggaatg aataaaaagg tcttgcctta ccatcataac
     5101 tacaaacaaa atcagctcga attataccga gtttttctaa tcccacagta agttcataat
     5161 gactttttat ttcattaact tttaagaaat ggaaacctac aatttcatta agcttcaact
     5221 ttcggattaa tggaatttta ttggtaataa aacggttaag attgtattct gcatggattg
     5281 caaaagcatc tctcgtattc gaatatttat aataatccaa ataagcaaaa tcttcagtcc
     5341 cgaaatctga tacgatagtc ttgttgcccc caaaatgaaa aaagtccgga aattcaacat
     5401 gctttcgatt aacaaaacga taatacctgc aattgtaaac tacaaaaccc aacatgccaa
     5461 agtctatctg atgtttaaaa cctgcatcaa atgaaagata atcagtctta ctgtttatca
     5521 cattgccaaa agctttattg acatgcataa ataacaaagg ccatcctgat tcttctctta
     5581 cttttccatc aggtcttgta atgaatttcg taaatggcga gtaggttaaa gtaagatgtg
     5641 tgttgaaata atgatgcggc agaaatattt ttatatcata atccggacta atcggattat
     5701 tggaagtata tgtcctgttt ttaaaattct tccaggtata atccgaagta tttgtcaatg
     5761 catttcttcg gccatattca atttctgcag ttgcattaaa tccattaaac cattctttac
     5821 cataagaaac ctttaaaaat tgcttctcat aaatttttaa gtagttacgt tcagcccaga
     5881 gtgtgtaaag catgtttact aaaggtttaa caggatttgt gctgttcagt tcattaacag
     5941 cagtgcctcc ttcaaaagaa attgtattat cattaaagcg attgaacaaa taattaaaac
     6001 ctgcatttgc attccaatgt gtattgctaa aaccataacg tgtgttagta aaaactgtca
     6061 gtcgttttcg gtttggtaac ctttttacaa attctgtatt caaagcaatg ttccagcctt
     6121 caactgtatt aaatgaaaag gtgttggcaa tgggttttat ggtataagtc atttcacgat
     6181 aactgtgcct gtaagaatat ccggcaagca aaaaattaaa aaccgaaaac ttattattta
     6241 ttctatccat gctgtcttta tattgtttgc tttctcgtat ggtagccaaa ctatctttct
     6301 taaaataatc tgatgtttca tcttgcgtaa gtggtatagg tcttacagta tcccaatatg
     6361 cagaatcttt cttgttggca tcaggactta tttttaaaat ttctgctgaa aaagttttgg
     6421 cagggaaaac aggatttatt ttaaagtcag aaaagtaacc tacataatta cctcctcctt
     6481 ggaaaccaag tatcctgaaa ctaaaatcga ataaatttgt aactggcatc caaacattat
     6541 tctctacagg aacaaaagac tgctttattc ttagcgtgtc taaaaactca atgcccgctt
     6601 cacgtgtaag gaaaagatca ataaactgaa atcgccataa atcatcagca atataaataa
     6661 cacctctgaa cacaggatca ttctgtcttc ttggggttac ctcaatttta tgtatgagtc
     6721 tgtcattctc tacaacagag ccaagtaatt tatagcggta ataaaacaaa gcatcaccgg
     6781 ctaccggtga cacaaatcct tttgaagaga taccttctga cataactctg ttttcataaa
     6841 aattaaattg catatcggaa gctttgttgt agctgaaggc tttattgtta ccactaaaac
     6901 gtgatgacac caactcctct ctgatttttc cgggtttttc atagctgtat tttgataccg
     6961 actcacttaa ataaaatatc cctgttgttg aatcaaccat accgccaaaa tcaacgacct
     7021 taccaagaaa tttttcagga tacttattta atttctgcac acctttgata tacacattac
     7081 atgaataggc atcaacttcg tttaaataac ttttccgttt agcaattgct tttctgatat
     7141 attcataggc agggtcttct ttattgtttg taactgcaac ctcctttaac gaatagagct
     7201 cttctgtgag ttctatctca atttgttgtt tacctgcacc aacttcaatt tgcttactga
     7261 attgcttata gcccagattc tgacaataaa gtgtgtagct accaggagca agttcaattt
     7321 caaaaatacc gtcattactg gtagttgtcc ctacaaaacc gggttttaca tatacattgg
     7381 cataagctac aggtttgtga tttttatctt tcacaacgcc tgatacaata ccttgagaaa
     7441 acactgctga actaaatata ttacccaaca aaatcaggca aaaaaattta cgcatcacaa
     7501 taattaattc ataatttaag gatgctaatg taaaacttta agttcttgtt ttaaattgtt
     7561 tccggaacct
//
LOCUS       DAOQNP010000104         7510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3706_length_7510_cov_9.603488, whole genome shotgun sequence.
ACCESSION   DAOQNP010000104 DAOQNP010000000
VERSION     DAOQNP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3706_length_7510_cov_9.603488"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1968
                     /gene="lysS"
                     /locus_tag="PKY09_11405"
     CDS             244..1968
                     /gene="lysS"
                     /locus_tag="PKY09_11405"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNB13505.1"
                     /translation="MALSEQEQIRREKLNELLQMGINPYPAAKFEVNSNSTFILENYE
                     KAETRFGAGKEEVSIGGRIMSVRDMGKAAFAQIQDAEGKIQIYIKRDEICKGDDKTMY
                     DTVWKKLIDIGDIIGIKGYVFKTKTGETSIHASSITLLCKSLRPLPIVKEKDGEVFDA
                     VIDPDLRYRQRYVDLIINPQVKETFRKRTQLVNTIRYYLNEKGYLEVETPILQPLYGG
                     AAARPFKTHHNTLDMTLYLRIANELYLKRLIVGGYDGVYEFSKDFRNEGMSRFHNPEF
                     TQIELYVAYKDYEWMMNLVEEMIEKVALALHGSTVVTVGDKSIDFKRPWKRYTMYEAI
                     EHFTGIDVSAMDEMAMRKAAVQMGIELDDTMARGKVIDEIFGSKCEPNLIQPTFITDY
                     PVEMSPLAKKHRSKPGLVERFEAICNGKEMCNAFSELNDPIDQRQRFEEQLELGKRGD
                     EEAMQLDEDFLRAIEYGMPPTAGLGIGIDRLSMIMTNAASIQDVLFFPQMKPETIETN
                     AASDTGLLHIPAPWNDVISRMGITSKQQLKEVNPNKLFNDMGGMRKKLKLDVSMVTLE
                     EVKSWINA"
     gene            2056..2583
                     /locus_tag="PKY09_11410"
     CDS             2056..2583
                     /locus_tag="PKY09_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNB13506.1"
                     /translation="MATKKIDLKAFRIKSGRNRKKYTTFLKGLHSRKVKQLTAKAKKL
                     NDLAFKKIDCLACGNCCKTMTPTWQPADIKRVAAYFKMTVQQFKDKWLYKDEAGDWMN
                     TKTPCQFLQKDNKCSIYEIRPKDCSGFPHLQHTDFLIGREVNIPNMTSCPITYEVVER
                     MYKLVVEKDATVLND"
     gene            complement(2592..5489)
                     /locus_tag="PKY09_11415"
     CDS             complement(2592..5489)
                     /locus_tag="PKY09_11415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNB13507.1"
                     /translation="MSFFKTVLLSCWLLGNSILYAQPTYTQYVNPFIGTGGHGHTFPG
                     ATLPFGMVQLSPDTRIDGSWDGCSGYHYSDSILYGFSHTHLSGTGVSDYGDILLLPMN
                     NEPALDESTPTALFSHNFENATPGYYAVTLLNDNINAELTATLRTGIHRYTFNKTGLA
                     YIYLDLNHRDKLVASNINIENDSTIKVYRKSEGWAKEQILYANIRFSKPFIQSKQLGN
                     KKAVFVFSVSEKEGIMAKVALSAVDDDGAERNMILENPDWNFEHIKINAAKKWNEELS
                     KIKVSSSDKNKLSIFYTAMYHCMIHPNIYNDVDGRYRGRDLQIHQTDHNYYTVFSLWD
                     TFRAWHPLMTIIDEERVTDFIRTFLLQYEQGGLLPVWELAANETECMIGYHAVSVIAD
                     AMMKGIKGFDYEKALEACKFSAEQRNRFGLGAYIDKGFLEVEDEHESVSKTLEYAYDD
                     WCIAQMAKLLGKEDDYNTYMHRSQSWKNLFDPVTGFIRPRSNGGWLNPFDPFEVNNNY
                     TEANGWQYNFFVPHDIDGMITYSGGKEKFEEKLDQLFSVSSKTTGREQSDITGLIGQY
                     AHGNEPSHHIAYLYNFVDKPGKAEKIINKVINDFYKNSPDGLIGNEDCGQMSAWYVMS
                     AIGMYPVCPGSNQYVLTTPQFDKVEILTSENKIFTIDTKNKTNKANFLKVKKTKNISF
                     RDGLLFNHNSFRTDNFLVLTADSIPSLLRKKDSADNIQYSDNFYRVPVITASTTVFKD
                     SLKVSIKSFQKDKSIVYLQQGPFERPAKIDYKKPFYIYDTSVIHAFVTDTTGNESKWA
                     KAEYFKLPHPKWKVSIKGNYNPQYNAGGANGLIDGLHGDANWRKGRWQGYQGQDFECV
                     IDMGNQQEISSFNTGFLQDTRAWILMPVSVEYLISDDGKLFNNVGIVQHQTAPDNYEI
                     SIENFKLQTEAPIKARYIKVVAKNFGKLPSWHQGFSMGGEAFIFVDEVEVE"
     gene            complement(5667..6251)
                     /locus_tag="PKY09_11420"
     CDS             complement(5667..6251)
                     /locus_tag="PKY09_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNB13508.1"
                     /translation="MKHYYVLYKPYGYLSQFSDEAKHKGLNHLIKVEPDVYPAGRLDV
                     DSEGLLLLTNDNFLKHHLLNPENKHKRTYHVQVDGTATSVHLNLLQQGVEITIEGKPY
                     KTLPPVSVNLLNEVSYPERNPPVRFRKHIPTSWIELVLTEGKNRQVRKMTAAVGIPTL
                     RLIRNKIEAINIKDYQQGELKELSQQEIYKLLKI"
     regulatory      6321..6426
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6509..7171
                     /gene="mnmD"
                     /locus_tag="PKY09_11425"
     CDS             6509..7171
                     /gene="mnmD"
                     /locus_tag="PKY09_11425"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNB13509.1"
                     /translation="MDSIEIVKTKEGIDTLINTRINEHYHSLHGAMQESMHVFIKNGL
                     LALNKNLPEIKILEMGFGTGLNAILTYRESYVLQKQIHYTTIESSPLPMAIINKLNYF
                     EYFGKALLPIFQRMHDCNWFENMTFGDFVLFKINADMLEFKLDGSYDLVYYDAFSPTH
                     QPELWSFEVLQKVYDSCNKGAMLVTYCANGEVKRTLKKVGFAVETLPGPKWKREMIRA
                     SK"
     gene            7203..>7510
                     /locus_tag="PKY09_11430"
     CDS             7203..>7510
                     /locus_tag="PKY09_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNB13510.1"
                     /translation="MKNIKIELEKRILVIDGAMGTMIQRYKLEEADYRGKRFADWKSD
                     LKGNNDLLSLTKPEIIAAIHLEYLEAGADIIETNTFNAQKISLADYHMESLAYELNFE
                     S"
BASE COUNT         2434 a         1314 c         1436 g         2326 t
ORIGIN      
        1 gacataaaaa aaccccgtca acattgctgc tgacggggga agttaaaccc accctgaact
       61 aaaattattt taacagagaa tagtctttaa ttagtgaatc aaaattaggt atccaatgct
      121 gtgcagtcaa atagtttgtc cctaacggga aattgtgagc tcctgaacgg gcaaaacagc
      181 tgtctgacct aaaagaataa atgcaagcgg caacttgtat ctttgtaatc aaataaaaaa
      241 ataatggcat tatctgagca agaacaaata agacgagaaa agctgaatga gctgttacag
      301 atgggtataa atccctatcc ggctgcgaag tttgaagtaa actccaacag cacctttata
      361 cttgagaatt atgaaaaggc agaaacacgt tttggtgcag gaaaagaaga agtgagtatt
      421 ggtggccgca tcatgagtgt aagagatatg ggtaaggctg catttgcaca aattcaggat
      481 gcagaaggaa aaattcagat atacattaaa cgtgatgaaa tttgtaaggg cgatgataaa
      541 accatgtacg ataccgtttg gaagaagctt attgatatag gcgatattat tggtattaaa
      601 ggttatgtct ttaaaacaaa aacaggcgag acatctattc atgcaagttc tattacatta
      661 ttatgtaaat cacttcgtcc attgcctatt gtaaaagaaa aagacggaga agtttttgat
      721 gccgttattg acccggattt aagataccgt cagcgctatg ttgatttaat tataaatcca
      781 caggtaaaag aaacattccg taagcgtaca cagttggtta ataccatacg ttattacctg
      841 aacgaaaaag gatatctcga agtagaaaca ccaattttac aacctctata tggtggtgct
      901 gcagcgcggc cattcaaaac ccatcacaat acattggata tgaccttgta tttgcgaatt
      961 gccaacgagc tttatctgaa aagattgatt gttggtggct atgatggtgt atatgaattt
     1021 tcgaaagatt tcagaaacga aggcatgagt cgctttcaca atccggagtt tactcagata
     1081 gagctttatg tagcctataa agattacgag tggatgatga acttagtgga ggagatgatt
     1141 gaaaaagtag ctttagcatt acatggatca acagtggtga cggtaggaga taaatctatt
     1201 gacttcaaac gtccatggaa gcgttatacc atgtatgaag cgattgaaca ttttacagga
     1261 atagacgtaa gtgcaatgga tgaaatggcc atgcgcaaag ctgcagtaca aatgggaatt
     1321 gaactggatg atacgatggc aagaggaaag gttatagatg aaatttttgg tagtaagtgc
     1381 gaacccaatc tcattcaacc tacatttata acagactatc cggttgagat gtcgccattg
     1441 gcgaagaagc accgcagcaa acccggattg gtggagcgat ttgaagctat ctgtaatggt
     1501 aaagaaatgt gtaatgcatt ttctgagttg aatgacccta ttgatcaacg tcaacgtttt
     1561 gaagagcaat tggaattagg aaaacgtggt gatgaagaag ccatgcaact tgacgaagat
     1621 tttcttcgtg ctattgaata tggtatgcct cctacggcag gtttaggaat tggaattgat
     1681 cgtttgagta tgatcatgac aaatgctgca agtattcagg atgtattgtt tttcccacag
     1741 atgaaacccg agaccattga aaccaatgca gcatcggata caggtttgtt gcatatacct
     1801 gcaccttgga atgatgttat ttccagaatg ggaattactt ctaaacaaca acttaaagaa
     1861 gttaatccca acaaattatt taacgacatg ggtggcatgc gtaagaaact gaaacttgat
     1921 gtaagcatgg taacacttga agaagtgaaa agttggatta atgcttaaac agattttaat
     1981 atagtatcct tcttgtttgc agcatgactt tggttatgaa ataaatttgt tcatttgcat
     2041 gcttgtttat aaactatggc aacaaaaaaa attgatttaa aagcatttcg aattaaatcg
     2101 ggcagaaaca gaaagaagta cacaactttt ttgaaaggac tgcatagccg caaggtaaaa
     2161 cagttgactg caaaagcaaa aaaacttaat gatcttgctt ttaaaaagat tgattgcctt
     2221 gcctgtggta attgctgcaa aaccatgaca ccaacatggc agcctgcaga tataaaaaga
     2281 gttgcagcat attttaaaat gacagtgcaa cagtttaaag acaaatggtt gtataaagat
     2341 gaagcaggcg actggatgaa tacaaaaacg ccctgtcagt ttttacaaaa agataataag
     2401 tgcagtattt atgagattcg tcctaaagac tgttccggat ttccacatct gcaacacaca
     2461 gactttttaa ttggtcgcga agtaaatatt cccaacatga ccagttgtcc gattacttat
     2521 gaagttgtag aacgtatgta taaattagtt gtagagaaag atgcaactgt actgaacgac
     2581 taagtacttt ttcattctac ttctacttca tcaacaaaaa taaaagcttc gccacccatt
     2641 gaaaaaccct gatgccatga aggtagtttt ccgaaatttt tagcaacaac ttttatgtag
     2701 cgtgctttta ttggagcttc cgtttgtagt ttaaaatttt caatacttat ttcataatta
     2761 tccggtgcag tctgatgctg aacaataccg acattattaa atagcttgcc atcatcagag
     2821 ataaggtact ccactgaaac aggcataaga atccaggctc ttgtatcttg taaaaaaccg
     2881 gtgttaaaag aagaaatttc ttgctgattt cccatatcaa taacacattc aaaatcctgc
     2941 ccctgataac cttgccagcg acctttacgc cagtttgcat cgccatgcag tccatcaatc
     3001 aatccgtttg caccacctgc attatattgt ggattgtaat ttcctttaat ggaaactttc
     3061 catttcggat gaggaagttt aaagtactca gcttttgccc acttactttc attacctgtg
     3121 gtgtctgtaa caaaagcatg aataactgag gtatcgtaaa tataaaaagg ctttttataa
     3181 tcaatctttg ccggacgttc aaaaggacct tgttgcagat atacaatgct tttgtctttc
     3241 tgaaatgatt ttatactgac tttaagtgaa tctttaaata cagttgtcga agcagtaatt
     3301 acaggaacac gataaaaatt atctgaatat tggatgttat ctgccgagtc tttcttacgg
     3361 agtaaagaag gaatgctgtc ggcagtcaga actaaaaaat tatcagttct aaaactattg
     3421 tggttaaaaa gcaagccatc tcgaaaagaa atatttttcg tttttttaac tttaagaaag
     3481 tttgctttgt ttgttttgtt cttagtatca attgtaaata ttttattttc tgatgttaaa
     3541 atttcaactt tatcaaactg tggagtagtc agcacatatt gattactacc cggacaaaca
     3601 gggtacatgc caatagcact catcacatac catgcactca tctgtccgca atcttcgttg
     3661 cctattaaac catcaggact atttttataa aaatcattta tcactttatt gattattttc
     3721 tcagccttgc ccggtttgtc aacaaaattg tacaagtaag caatgtgatg actgggctcg
     3781 ttaccatgtg catattgtcc tattaatcca gttatatctg attgctctct accagtagtt
     3841 tttgaagaaa cactaaaaag ctgatctaat ttttcttcaa acttttcttt tccgccactg
     3901 taagtaatca tgccatcaat atcatgagga acaaaaaagt tgtattgcca tccgtttgct
     3961 tcagtgtaat tgttatttac ttcaaaaggg tcaaaaggat ttagccagcc accattgctt
     4021 ctgggtcgga taaagccggt cactgggtca aataaatttt tccatgactg cgagcgatgc
     4081 atataggtgt tgtaatcatc ttcttttccc agcagcttag ccatttgtgc aatgcaccaa
     4141 tcgtcatagg catactctaa tgttttagaa acagattcat gttcatcttc gacttctaga
     4201 aaacctttgt caatgtatgc gcctaatcca aaacgattgc gttgttctgc actaaactta
     4261 catgcttcaa gcgctttttc atagtcgaaa ccttttatgc ctttcatcat tgcatcagca
     4321 ataacactta ctgcatgata gccaatcata cattcagttt catttgctgc cagttcccaa
     4381 accggcaaaa gccctccttg ttcatactgc aataaaaagg tgcgtataaa atctgttaca
     4441 cgctcctcgt caatgatagt catcaacgga tgccatgccc tgaaggtatc ccaaagagaa
     4501 aaaacagtgt aataattatg gtcagtctga tgaatttgta aatctcttcc gcggtatctg
     4561 ccatcaacat cattataaat attaggatga atcatgcaat gatacattgc agtatagaaa
     4621 atggataact tatttttatc actgctgctg acttttattt tcgatagttc ttcattccat
     4681 ttttttgctg catttatttt tatatgttca aaattccagt ccggattttc gagtatcata
     4741 tttcgttcag caccgtcatc atcaactgct gataatgcca ccttggccat gatgccttct
     4801 ttttcagaaa cagaaaaaac aaatacagcc ttcttattac ccaattgttt tgattgaata
     4861 aaaggttttg aaaaacgaat attagcataa agtatttgct cctttgccca gccttcactt
     4921 tttctgtaaa ctttaatggt ggaatcgttt tctatgttaa tgttactggc aacaagctta
     4981 tcgcgatgat ttaaatcaag atagatataa gctaaccctg ttttgttaaa tgtgtagcga
     5041 tgtatgcctg ttctcaatgt tgcagtcagc tctgcattta tgttgtcgtt aagtaaagta
     5101 acagcataat agccgggtgt ggcattttcg aagttatgtg agaataatgc tgttggagtg
     5161 ctttcgtcca atgcaggttc attgttcatg ggtagtaata aaatatcacc atagtcactc
     5221 actcccgttc cacttagatg cgtgtgcgaa aatccataaa gtatagagtc agaataatga
     5281 taaccactgc agccatccca acttccgtct attctggtgt ctggactcag ttgaaccata
     5341 ccaaagggga gtgttgctcc cggaaaagta tgcccatggc cacctgttcc aataaatgga
     5401 tttacgtatt gagtgtaggt tggctgtgca taaagaatgc tgttgccaag caaccagcag
     5461 cttagtaaaa cagttttaaa aaacgacata acaaacagta ataatcagat aaaagtataa
     5521 atagttttga aattgagaaa ctgaagttaa ttaatccaaa ttttattacc atgacagata
     5581 aaaatatttt ccggtctgtc agctgatcaa aaattcttta ttttaaagga atacaattgt
     5641 tttttgaaaa gaatttttag gaaaacttat atctttagta acttataaat ttcttgctgc
     5701 gacaattctt ttaattcacc ttgttggtaa tctttgatat ttatcgcttc aattttattt
     5761 ctgattagcc tgagagtagg aataccaaca gcagcagtca tttttcttac ctgacggttt
     5821 ttaccttctg taagaaccag ttcaatccaa cttgttggaa tgtgtttacg aaagcgcacg
     5881 ggaggatttc tttcaggata tgaaacctca ttcaataagt ttaccgatac aggaggcaac
     5941 gtcttatacg gcttaccttc aatggtgatt tcaacacctt gctgaagcag gttaagatgt
     6001 acacttgtgg cagtgccatc aacctgcaca tgataggtgc gtttatgttt gttttcagga
     6061 tttagcaaat ggtgtttcag aaaattatcg ttggtaagta gcagcagtcc ttcgctgtca
     6121 acatcaagtc gtcctgcagg ataaacgtct ggttcaactt taatgaggtg atttagccct
     6181 ttgtgtttgg cttcatcact aaactgtgac aggtagccat aaggtttata aagtacatag
     6241 tagtgtttca cgatttaaaa atacaaccta ttttgaaata caccttgttt atgctatctt
     6301 tgtagcagtt ctttttcatc agcttatcga gaaaggcgga gggacgtgcc ctgcgatgcc
     6361 ttgacaacca actgccattg gcagaaaagg tgccaattca cattccctaa gggaagcaga
     6421 taagtcgaaa tcaggttaaa atcgtttcaa tctttttcgg ctttgcggaa aagatttttt
     6481 tattttaaat cggattaaaa tttaggagat ggactccatt gaaattgtaa aaacaaaaga
     6541 aggtattgac accttaatca atacccggat taatgaacac tatcattcat tgcacggagc
     6601 tatgcaggaa agtatgcatg tttttattaa aaatggtttg cttgcattga ataaaaacct
     6661 tcctgaaata aaaatacttg aaatgggatt tggcacagga ctcaatgcca tactcactta
     6721 cagagaaagt tatgtgttgc agaagcaaat acattataca actattgagt cttcaccgct
     6781 gccaatggca ataataaaca agttaaatta ttttgaatac ttcggaaagg cattacttcc
     6841 catctttcaa agaatgcatg attgcaactg gttcgagaat atgacttttg gtgattttgt
     6901 attatttaag attaatgctg atatgttgga gtttaaatta gatggttctt atgatcttgt
     6961 ctattatgat gccttttcac caacacacca gccggagttg tggtcttttg aagtacttca
     7021 gaaagtttat gattcatgca ataaaggagc tatgctggta acttattgcg ccaatggtga
     7081 agtaaaacga acgttgaaaa aagttgggtt tgccgttgaa acgttgccag gaccaaaatg
     7141 gaaacgtgaa atgataagag ccagtaaata aattagaaaa aattgttgca cactaaatta
     7201 gaatgaaaaa tattaagata gaattagaaa agagaatatt ggtgattgat ggtgccatgg
     7261 gcacaatgat acagcgatac aaacttgaag aagcagatta tcgcgggaaa agatttgcag
     7321 actggaaaag tgatttaaaa gggaataacg atttgctctc acttactaag cctgaaatta
     7381 ttgcagccat tcacttggaa tatctcgaag caggtgcaga tattattgaa acaaacacct
     7441 ttaatgcaca gaaaatttca ttagctgatt atcacatgga gtcattagct tatgaattga
     7501 attttgagtc
//
LOCUS       DAOQNP010000105         7459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3743_length_7459_cov_7.882091, whole genome shotgun sequence.
ACCESSION   DAOQNP010000105 DAOQNP010000000
VERSION     DAOQNP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3743_length_7459_cov_7.882091"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKY09_11435"
     CDS             complement(<1..322)
                     /locus_tag="PKY09_11435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNB13511.1"
                     /translation="MHIRQATTDDCSAISTLAHSIWHSHYPGIITVQQIDYMLSKMYS
                     VEKLNNLLESGSVILVAEEDEKLLGYICFREETENSFFLDKLYVDTALQHKNLGSLLL
                     EAMLS"
     gene            complement(326..889)
                     /locus_tag="PKY09_11440"
     CDS             complement(326..889)
                     /locus_tag="PKY09_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNB13512.1"
                     /translation="MNILVIDNFDSFTYNLVHYIEKAAGVRPTVWRNNSHTLDEVAQF
                     DKVCISPGPGLPSQAGITKEVIKRFASEKSILGVCLGHQAIAEVFGGSLRNLQQVLHG
                     VVRPVHVLVQDVLFENVPSTFPAARYHSWIVDQTGNQLQVIAGDDIGEVMAIKHIQYP
                     VYGVQFHPESIMTEHGLQLIHNWIHKT"
     gene            complement(925..1632)
                     /locus_tag="PKY09_11445"
     CDS             complement(925..1632)
                     /locus_tag="PKY09_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB13513.1"
                     /translation="MADQNLRILLVEDEQTLSETIKLNLEMEGYYVNAVGDGKTAIKV
                     FKEARYNLVILDVMLPEISGFTVCEAIRLENLDVPILFLSAKNHPNDRITGLKLGGDD
                     YLTKPFNLEELLLRVHNLIKRSTASKEVVALSTYNLNNNQINFAQLEIKRPDGTITHL
                     TKKESLLLKLLIEKRNEVVSREHILETVWGYDIYPSTRTIDNFIVTFRKYFEPDPSNP
                     KYFISVRGVGYKFTDQV"
     gene            1732..3231
                     /locus_tag="PKY09_11450"
     CDS             1732..3231
                     /locus_tag="PKY09_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13514.1"
                     /translation="MFYSLLFLFVYGYKFNCSDLEEHLPAVYKMLNPHLYHGDYVTEF
                     LASDFSVRYFYNHTLYYPGLVFSLSTVAFTLMLMLLTVFTYYMQKTSFVIFKNHITAS
                     ITPFIAIILLNKITIGGNTVSDNMLTCTVFANTMCMAAFYYGLSSRLRIAALCCGLAT
                     WYQPLIGMQVFLLLMLMHSVINKFRLKEIGITIIFYFLSASPMLLPLFNRQFFAESAT
                     PDMLFYYVLYVFRNPHHYLPSFFPVADYIKSGILFLISGLLFFRFPTKYNRQIMVIIS
                     GIVAGMIFYYLIFDAGGMMIVGKLQWFKTSVWLTIFCSFYIAHFIAQQNLTLKILQCK
                     CVTASIITLSILIFYLITHASLLPLEKLKTRYTIGQFKKTDLMLMHEWIRENTPVTTK
                     ILSFPDDDSFLCEAQRPTPVAWKAIIHEPWFLKSWYYTFTTDFNLDRKIEWKNINQRE
                     KASVAFLNINHKPADASYTMILLNANKLPEQGKGKIIHRQGEYTLWQWN"
     gene            complement(3235..4704)
                     /locus_tag="PKY09_11455"
     CDS             complement(3235..4704)
                     /locus_tag="PKY09_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB13515.1"
                     /translation="MRKIFLLIVLMCISNLISAQSSDRKWNLGLYGGATQYNGDMGQG
                     FYKTNQPFYGFGAISVGYYLGKYADLQIIGTTGEAGFIEDQVNRFRVKMTTGSLHLRF
                     HFTKPEAALRPFLFAGAGIIVWDRNIWAQNGDVINRYDYALPSFGGGLNFRLSESFNL
                     VVQETFMLSSEDDVDRQVNQNNDGFLFHTVGLTFNLGKNKDSDDDGVADKKDNCPNTP
                     KGIKVDASGCPVDSDNDGVADYMDDCPTAAGSQQLKGCPDRDGDGISDKDDLCPDAKG
                     LANLNGCPDEDGDGVADNNDKCPETKKGYKVDANGCPFDNDSDGLVNEEDMCPDVPGV
                     VALKGCPDSDGDGVADYEDRCPTIKGTIANKGCPEIAKEDVKKITQIASSIYFESNSD
                     KLKPASLIQLDALVGILKKYDAANLSIEGHTDNKGDDAYNLNLSQKRCDAVKNYLMSK
                     GIFESRLTATGFGETKPITGNDTPEGRAKNRRVELKTSY"
     gene            4867..5961
                     /locus_tag="PKY09_11460"
     CDS             4867..5961
                     /locus_tag="PKY09_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNB13516.1"
                     /translation="MRIKEVINVLENWASPAYQESYDNCGLLTGNSADEVKGIMVCLD
                     STEDVLDEAIKENCNLIIAHHPIVFSGLKKITGRNYVERVIIKAIKNNLALYALHTNL
                     DNIETGVNMRLCHQLGLTNAKILLTKQGTLKKLVTFTPIAQAEKVRTALFEAGAGNIS
                     NYSECSFNVEGKGTFKPEAEANPVIGEKLIRHTESEVRTEVIFEAHKEYQVLKALFES
                     HPYEEVAYDIYALTNSHQQTGSGMIALLEDEMDEIAFLNMVKTKLNCSALKHTRLCHK
                     KVRKVAVCGGSGFFLLPQAIAAGADAFITSDIKYHQFFDADGRILLIDAGHYETEQFT
                     MMLIKDLLIKNFTTFAIRLTKVDTNPVKYI"
     gene            6486..7253
                     /locus_tag="PKY09_11465"
     CDS             6486..7253
                     /locus_tag="PKY09_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNB13517.1"
                     /translation="MPKKGVHDASAEEKLQALFQLQQIDSEIDRIQIVRGELPVEVKD
                     LEDDVARLETRISNINNDMAEFDRLIAERKQGIKDSTAAIKKYEGQQNNVKNNREYES
                     LAKEIEFQKLEIQLCEKRIKEYTFEITSKKQLLTESEEDLTHRKADLENKRKELDSII
                     AETRKEEDVLNEYSSKAAAIIEERLLSGYKKIRENAANGLAVVSIQRDSCGGCFNKVP
                     PQRQLEIKQRKKVIVCEHCGRILVDQEIMTDLPENPA"
     gene            7382..7456
                     /locus_tag="PKY09_11470"
     tRNA            7382..7456
                     /locus_tag="PKY09_11470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7417..7419,aa:Val,seq:tac)
BASE COUNT         2459 a         1415 c         1347 g         2238 t
ORIGIN      
        1 tactcaacat agcttcaaga agtaaagagc ctaagttttt atgttgcagc gctgtgtcca
       61 catatagttt atcaagaaaa aaactatttt cagtttcctc acgaaagcaa atatagccta
      121 aaagtttttc gtcttcttct gcaaccaaaa ttactgaacc tgactcaagc agattgttca
      181 acttttcgac actgtacatt ttgctcagca tgtaatcaat ctgctgtacg gtgataatac
      241 cggggtaatg actatgccaa atgctatgtg ctaaagtgct gatagcacta caatcgtctg
      301 ttgtagcttg tctaatatgc atgaatcaag ttttatgaat ccaattatgt ataagttgca
      361 gcccatgttc agtcataatg ctttcaggat gaaattgcac tccataaaca gggtattgaa
      421 tatgctttat agccattact tcgccaatgt cgtcaccggc aataacctga agttgatttc
      481 ctgtttgatc cacaatccat gaatgataac gtgctgcagg aaatgttgaa ggaacatttt
      541 caaacaacac atcttgcacc aaaacatgca cgggtcttac taccccatgc agaacttgtt
      601 gtaaattacg aagactgccg ccaaaaactt ctgcaatagc ctgatgacct aaacaaacac
      661 caagaattga tttttctgat gcaaatcgtt ttattacctc cttggtaatg cctgcctgag
      721 aaggtaaacc gggtcctgga gaaatgcaaa ccttatcaaa ttgagcaacc tcatcaagag
      781 tatgtgaatt atttcgccaa acagtaggtc gaacacctgc tgccttttca atgtaatgga
      841 caaggttgta ggtaaaagaa tcgaagttat caatgactaa aatattcacc tgtcaaaaat
      901 agcgaatgtt ttaaggagtg gcgctcaaac ctgatcggta aatttgtaac caacacccct
      961 gacagagata aaatatttag ggttggaagg gtcaggttca aaatatttcc tgaatgtaac
     1021 gataaagtta tctattgttc gcgtgcttgg gtaaatatca tagccccaaa cggtttccag
     1081 aatgtgttcg cgtgaaacaa cttcattgcg tttttctatg agcaatttca aaagcaatga
     1141 ttcttttttt gtcagatgag taattgtacc atcaggacgt tttatttcaa gttgcgcaaa
     1201 attaatttgg ttgttgttca ggttataagt tgaaagtgcc accacttctt tgcttgcagt
     1261 acttctttta attagattat gcactctgag caatagctct tcaaggttaa atggcttggt
     1321 cagataatca tcgccaccta atttaagacc tgtaattctg tcgttggggt ggtttttggc
     1381 tgaaagaaat aaaatgggaa catcaagatt ttcaaggcgt attgcttcgc atacagtaaa
     1441 tccgcttatt tccggtagca taacatctaa aatcactaag ttataacgtg cttctttaaa
     1501 cactttaatg gcggttttac catcgccaac agcattcaca tagtagcctt ccatttcaag
     1561 gttaagtttt attgtttcag acaaggtttg ctcatcttcc accaacaaaa ttcttagatt
     1621 ttgatcagcc atatcaattt tttacgatgc taattaacaa aaatgttttc tttgtttatt
     1681 atgttgcgga attattcagt gtcacaatcg ggtaaatatt ggatttggtc catgttttac
     1741 agcttattgt ttctatttgt ttacggctac aaattcaatt gcagcgacct tgaagagcat
     1801 ctgcctgcag tttacaaaat gttgaatccg catttatatc atggtgatta cgtgactgag
     1861 tttctggcat ccgatttttc ggtacgctac ttctataatc acactttata ttaccccgga
     1921 cttgtgtttt cattatcaac agttgcattt acactgatgc tgatgctgct gactgttttt
     1981 acttattaca tgcaaaaaac atcatttgtg atttttaaaa atcacattac agcttcaata
     2041 acaccattta tagcaatcat cctattgaat aaaatcacta ttggtggaaa cactgtttca
     2101 gacaatatgc taacctgtac agtatttgct aatacaatgt gtatggcagc attttattat
     2161 ggactaagtt cacgactgag aatagctgca ctctgctgcg gactggcaac atggtatcag
     2221 ccacttatag gtatgcaggt atttctattg ctgatgttga tgcattcagt gataaacaaa
     2281 ttcagactta aagaaatcgg cattacaatc attttctact ttttgtcagc ttcgcctatg
     2341 ttgctgcctt tattcaaccg tcagtttttt gctgaaagcg caacacctga tatgcttttc
     2401 tattatgtat tatatgtttt cagaaacccg catcattatc ttccttcctt ttttccggtt
     2461 gcagattata ttaaaagtgg tattttattt ctgatatccg gcttgttgtt ttttagattt
     2521 ccgacaaaat acaacagaca aataatggta ataatttcag gcattgttgc cggaatgatt
     2581 ttttactatt taatttttga tgctggcgga atgatgatcg ttggtaaatt acaatggttt
     2641 aaaacttctg tttggcttac tatcttctgc agtttttata ttgcacattt cattgcacag
     2701 caaaatctga cactaaaaat cttgcaatgc aaatgtgtga ctgcaagcat aattacgcta
     2761 tcaattttaa tcttttactt aattacacat gcatcattat tacccttaga aaaactaaaa
     2821 actcgttata caattggtca gtttaaaaag actgatttaa tgttgatgca tgaatggatc
     2881 agagaaaaca cacctgtcac tacaaagatt ttaagctttc ctgatgatga ttcattttta
     2941 tgtgaagcac aaagacctac gcctgtggca tggaaagcca ttatccatga gccctggttt
     3001 ctgaaaagtt ggtattatac attcacaacc gattttaatc tggatagaaa aattgaatgg
     3061 aaaaatatta accaaagaga aaaggcaagt gttgcattct taaacataaa tcataaacct
     3121 gccgatgcaa gttatactat gattctatta aatgctaata aactgcctga acaaggcaag
     3181 ggtaaaataa ttcaccgtca gggagaatat accctatggc aatggaatta aaaatcaata
     3241 agatgttttt aactctactc ttctgttttt tgctcttcct tcaggagtat cgtttcctgt
     3301 tattggcttc gtttcaccaa aaccggtagc tgttaatctc gattcaaaaa tacctttgct
     3361 cataagataa ttctttacag catcgcaacg tttttgcgaa aggtttagat tatatgcatc
     3421 atcgccctta ttatcggtat gaccttctat tgacaggttt gctgcatcat acttcttcag
     3481 gattccaacc aatgcatcaa gttgaatgag tgaagcaggt ttgagtttat cgctgttact
     3541 ttcaaaataa attgatgaag caatctgagt aatttttttc acatcttctt ttgcaatttc
     3601 aggacagcct ttattggcaa tagtgccttt aatggtgggg catctgtctt cgtagtctgc
     3661 aacaccatca ccatcgctat cagggcaacc tttcagggca acaactccgg gaacatcagg
     3721 acacatatct tcttcgttta ccaacccatc gctatcatta tcaaaaggac aaccgtttgc
     3781 atcaacttta tagccttttt tcgtttcagg acacttgtca ttgttatcag caacgccatc
     3841 accatcttcg tcagggcatc cgttaagatt tgcaagcccc tttgcatccg gacataaatc
     3901 atctttatca ctaataccat caccatctct gtcagggcaa cctttcagct gctgagaacc
     3961 cgctgctgta gggcaatcat ccatataatc ggccacacca tcattatctg agtcaacagg
     4021 gcaacccgat gcatctactt taatcccctt aggtgtattc gggcaattat cttttttatc
     4081 tgcaacgcca tcatcatcag aatccttgtt cttgccaagg ttaaatgtta aaccaacagt
     4141 atgaaataaa aatccatcat tgttttgatt cacttgtcta tccacatcat cttcggagga
     4201 gagcataaat gtttcctgaa caacaagatt aaacgattcg gaaagtctga aatttaaacc
     4261 gccaccaaaa gaaggaagtg cataatcata acgattgatg acatcaccat tttgagccca
     4321 aatatttcta tcccacacta ttattcctgc tccggcaaat aagaatggtc ttaatgcagc
     4381 ttcaggtttt gtgaaatgaa aacgcaagtg caaacttcct gttgtcattt ttactctgaa
     4441 acgattaacc tggtcttcaa taaatccggc ttcacctgta gttcctatta tttgtaaatc
     4501 ggcatatttt ccgagataat atcctacaga aatagcaccg aaaccataaa atggctgatt
     4561 ggttttataa aaaccctgtc ccatatcacc attatactgg gtagctccgc catataagcc
     4621 taaattccat ttgcggtcac tactctgtgc ggaaatcaga ttgctgatgc acattaaaac
     4681 aattaacaag aatatttttc tcatttctta atttttgatt tgaaaactac acaaagtaaa
     4741 tatgctgttg gcatgataaa atggtgatgt aaaaaattaa ttaactacta attgtttaat
     4801 ttaattaact tttaacctct tgaaagcaac ttacatgctt acttttgcag ggcagaaaaa
     4861 ataatgatca gaattaaaga agtcatcaat gttttagaga actgggcatc tccggcatat
     4921 caggaaagtt atgacaactg cggacttctg acaggcaatt cagctgatga ggtaaaagga
     4981 ataatggttt gccttgactc tacagaagat gtattagatg aagccattaa agaaaactgc
     5041 aacttgatta ttgcacatca tcccatagta tttagtggac taaaaaaaat tacaggcaga
     5101 aattatgttg agcgtgtaat cattaaagcc ataaaaaaca accttgcact ttatgcacta
     5161 cacacaaatt tagacaacat tgaaactggt gtaaatatgc gattgtgcca tcagttgggt
     5221 cttacaaatg caaaaatatt gttgaccaaa caggggactt taaaaaaatt agtaaccttt
     5281 accccaattg ctcaggcaga aaaagtcaga actgcacttt ttgaagcagg tgccggaaac
     5341 atcagtaatt acagcgaatg tagttttaat gttgagggca aaggcacatt taagcctgaa
     5401 gccgaagcca atcctgttat cggagaaaag ttaatcagac acactgagag tgaagttcgt
     5461 acagaagtta ttttcgaggc acataaagag tatcaggtgt tgaaagcttt atttgaatct
     5521 catccatacg aggaagttgc atacgatatt tatgctttaa ccaacagcca tcaacaaacg
     5581 ggttcaggga tgattgctct tcttgaagac gagatggatg aaatagcatt tttgaatatg
     5641 gtgaagacta agctgaattg tagtgcatta aaacacaccc gcttatgcca taaaaaagta
     5701 cgtaaagttg ccgtttgcgg tggttcaggt ttttttcttt tacctcaggc aattgcagca
     5761 ggagcagatg ctttcatcac ttcagacata aaataccatc agttttttga cgctgatggc
     5821 agaattttac tcattgatgc cggacattac gaaaccgagc aatttacaat gatgctaatt
     5881 aaagatttgt taatcaaaaa ttttactaca tttgccatcc gtttaacaaa agtggatact
     5941 aatccggtaa aatatatctg attatggcag caaaaaaacc tgtgaaagct gtagcaaaaa
     6001 aatcaagtgc gaaaccggca gccaaaaagg cagcagtaaa aaaagcacct gttaagaaga
     6061 aggctgttgc aaaaccggcc gctaaaaaac ctgctgtaaa aaaagttacg gcaaaaaaag
     6121 ctgctacggc aaaaaaagct gttacggcaa aaccggctaa aaagtctgct tcaccaaaga
     6181 ccacaaaaac taaagctact gtaaaagaaa agaaaagtat aaaggccaga ccaaaagaaa
     6241 cgaatacaaa agtcacatca tccaaagaag taaaaaaaac aagtacaggc atgtcaaaaa
     6301 cagacactaa agcaaaaaca aaaagcagaa gtaagaaagt agaagaaccg gtaattacca
     6361 aggtgagcaa accggtagtg aaacgcttaa cggaaacgcg taacttaaaa gctattgata
     6421 caggtacagc agctaaatta tcaagcaaat acagtgttgg cgacatttct cagtttcaga
     6481 ttaaaatgcc aaagaaaggt gttcatgatg cctctgcaga agaaaagctt caagccttgt
     6541 ttcaacttca acaaattgac tcggaaattg acagaattca gattgtaaga ggtgaattac
     6601 ctgttgaggt aaaagatctt gaagatgatg ttgcacgtct tgaaacaaga ataagcaata
     6661 tcaataatga tatggcagag tttgacaggc tgattgctga aagaaagcaa ggcattaaag
     6721 attcaactgc tgcgattaag aaatatgagg ggcagcaaaa caatgtgaaa aacaacagag
     6781 agtatgagtc gcttgcaaaa gagatagagt tccagaaact tgaaattcaa ctttgtgaga
     6841 aacgaattaa agaatacact tttgaaatca catctaaaaa acaactcctc acagaatcag
     6901 aagaagattt aacacatcgc aaagctgatt tggaaaacaa acgcaaggaa ttagacagca
     6961 tcattgcaga aacccgtaag gaagaagatg ttctgaatga atattcaagc aaagctgctg
     7021 caattattga agagcgttta ttgagtgggt ataaaaagat tcgtgagaac gctgccaacg
     7081 gcttggctgt cgtttctatt cagcgcgata gctgtggtgg ttgttttaac aaagttccac
     7141 cgcaacgtca gttggaaatt aaacaacgca aaaaagtaat cgtatgcgag cattgcggcc
     7201 gtattcttgt agatcaggaa attatgacag acttgccgga aaatcctgca taaatagata
     7261 acaataaaaa agccgcttaa aagcggtttt tttatgccat atttttaaaa taaatttgga
     7321 gaatacttac ctatctgtat ctttgcacgc tcacaaaaaa acgacctatt aaaaatagtt
     7381 agggagcata gctcagctgg ttcagagcat ctgccttaca agcagagggt cacaggttcg
     7441 aatcctgttg ctcccacaa
//
LOCUS       DAOQNP010000106         7288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_3868_length_7288_cov_7.923960, whole genome shotgun sequence.
ACCESSION   DAOQNP010000106 DAOQNP010000000
VERSION     DAOQNP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_7288_cov_7.923960"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PKY09_11475"
     CDS             complement(<1..1190)
                     /locus_tag="PKY09_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNB13518.1"
                     /translation="MRYLNNAKKSILLIFVNILIGISCYAQMGVLKGNIYLKQSGEPV
                     AGAGIYPVNDKSLGVASDADGSYEINLPVGKQTIVYTLTGLKNDTLTLDIKDGTEAIR
                     NIYLTSNLKNLNVVVVSASKYEQRIEDITVSMEVISPKLIENRNTTNVTSVLEQTPGV
                     TILDQEPQIRGGSGFSFGVGSRVATLIDGLPIMTGDAGRTEWQFIPVENIEQIEVIKG
                     ASSVLYGSSALSGTINFRTAYPKERYHTYLRTYGGVYDKPSNREAKWWDGPATFSGIN
                     FLHSEKINRWDLIIGGNGLYDHGFIGPPLAMSSLPFQDTTISNNDVASRWGRLNFNIR
                     HRFKNIEGLSAGINGNFMQSHSNFSLVWANDSTGIYNAFPKTMTLTDSKTFYIDPYIS
                     LTGK"
     gene            complement(1192..2076)
                     /locus_tag="PKY09_11480"
     CDS             complement(1192..2076)
                     /locus_tag="PKY09_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13519.1"
                     /translation="MKRLYTTIFLILTAISIKAQNATPNAGFENWNQVGNRFDPADWN
                     NLNPTTGIIGVLTCARASGADAHTGNYAIKLTTKLIPGFNVIANGIASTGTLITTPPY
                     GVSGGIAYTGRPDSITGWYKYTPAGTDQGFVELQLLGATNTDTIGYVRFETPNATIST
                     YTRFAAAVNYLSTATPSNSIWILSSSKGVGPIVNSQIFIDDIAMVFNPSSVNSIDTKN
                     EVTILGNIIKEKIRFNYKNTGVYQFKLIDTAGRIVKETQLNSQQSTVDVSSLDGLYFY
                     SIENKSGIINNGKVIIRN"
     assembly_gap    2286..2385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2386..2682)
                     /locus_tag="PKY09_11485"
     CDS             complement(<2386..2682)
                     /locus_tag="PKY09_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13520.1"
                     /translation="MAKKYSFFQWLLSFLYPVIIQKDQSDVNSVLETTLENGKLVLNT
                     AHANYSYGSLYRVFIKALLRFNLDIKNKKKVLVLGMGGGSVVKFLLENNTLAQLD"
     gene            complement(2790..3383)
                     /locus_tag="PKY09_11490"
     CDS             complement(2790..3383)
                     /locus_tag="PKY09_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009746060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNB13521.1"
                     /translation="MKLISLFCISLLMLVSCKEKTESKQDTEVTEQLPSNSIFNLTSD
                     WQNQDGKTIKLNDLQGKALVVVMIYTTCKTACPILVAKMKTIESKFDRKDIDKINLVL
                     VSIDPETDTPEHLKEFSIKNEMTGPQWTFLRSDENATKEFANVLSMKYKKISPIDFSH
                     SNIISTFKPNGILVSQEEGDIQVDKVVATVNETVRKK"
     gene            complement(3396..>3794)
                     /locus_tag="PKY09_11495"
     CDS             complement(3396..>3794)
                     /locus_tag="PKY09_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNB13522.1"
                     /translation="DEWEFVAMADGDTANARSKAKYSDDIIDLYLQKNRQFKSVKQSP
                     PNYWGIYNMFDLIWEWTDDFNAVLTTGDSRSGEYNDKNLFCAGGATSATDVMNYAAYM
                     RFALRTSIKADYTIGNLGFRCAKDTTLINQ"
     assembly_gap    3795..3894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4096..5103)
                     /gene="nirK"
                     /locus_tag="PKY09_11500"
                     /pseudo
     CDS             complement(4096..5103)
                     /gene="nirK"
                     /locus_tag="PKY09_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
     gene            complement(5149..6513)
                     /locus_tag="PKY09_11505"
     CDS             complement(5149..6513)
                     /locus_tag="PKY09_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNB13523.1"
                     /translation="MLSLNKVKNAINSGLFVLFLSFIFPTDSQAQDTLKSENNFNIRG
                     QLRTRFELRDGAFRPLTKKEQPATLVSERIRLTFDYAYKDLFTIKISPQTVGVWGQAN
                     MVQGAEYSGNQFALFEAWSKINLSPSVNIKIGRQVITLDDERFFGELDWAQGGRAHDA
                     ISIDFHRKSFELRGYASFNQNYKVMYANNLNNPSGNLYSTTDGFPYKWMQTIWANIPA
                     GKYFRFTVLANNLGFQQATYSTTDTNTIFTQTFGLNNYFKKNKTDIHIAAYYQYCDNS
                     QTINTNAFLITANAAFQINNKFNLGLGSDYVSGNDVGQTNKINHAFNPYFHTGHKFYG
                     NMDYYYAGNPHKNTGISDSYLKVNYKTTGKLVLNAALHQFISPNKISDSYNKKYNSNL
                     GQELDLSFSFKTNTFVTFTGGYSFYLATTTLKYLKNTPGANDYQQWFWLSLNVTPHFL
                     KTNF"
     gene            6867..>7288
                     /locus_tag="PKY09_11510"
     CDS             6867..>7288
                     /locus_tag="PKY09_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNB13524.1"
                     /translation="MNLEQKIMAELKTAMLAKNEAALRALRAVKSAIILAKTSGSNVE
                     LSEADEMKLLQKLVKQRKESLDIFTQQNRSDLAKNEADEIAVIEKFLPAMMSADEVKE
                     VLKGIIASMGATQPSDMGKVMGAASKQLAGKADNKMIS"
BASE COUNT         2144 a         1240 c         1280 g         2424 t
ORIGIN      
        1 ttcttgcctg tcaggctgat gtatgggtca atataaaatg ttttagaatc ggtaagtgtc
       61 attgttttcg ggaatgcatt gtaaatccct gtgctgtcat ttgcccatac caaagaaaaa
      121 ttggagtgcg actgcataaa atttccgttg ataccagctg aaagtccttc aatatttttg
      181 aaacgatgac ggatgttaaa gtttaacctg ccccagcggg atgcaacatc attattgctg
      241 atagttgtat cctgaaaagg caaagaactc attgctaaag gagggccaat aaatccgtga
      301 tcatataatc cgttacctcc aatgattaaa tcccagcgat tgattttctc actatgtaaa
      361 aagttgatac ctgaaaaagt tgcagggcca tcccaccact ttgcttctct gtttgatggt
      421 ttatcataaa caccaccata ggttcttaag taggtgtgat aacgctcttt tggatatgct
      481 gtcctgaaat taattgttcc acttaatgct gatgagccat ataaaactga agaagcacct
      541 ttgatcactt caatctgttc aatattttct acaggaataa attgccattc tgtacgtcct
      601 gcatctccgg tcataatagg aagaccatca atgagtgtag ctacacggct gccaacacca
      661 aaactaaaac cactgccacc gcgaatttga ggctcctgat ctaaaattgt aacacccgga
      721 gtttgctcta acacactggt aacatttgtg gtgtttctgt tttcaataag ttttggactg
      781 ataacttcca tgcttactgt aatatcttca atgcgttgct cgtatttact tgcagaaaca
      841 acaactacat tcagattttt aagatttgat gtaagataga tattgcggat agcctctgta
      901 ccatctttaa tatctaatgt aagtgtatca tttttcagtc ccgtcagtgt atatactatg
      961 gtttgttttc caacaggaag atttatctca taacttccat cagcatctga tgccacgcct
     1021 aatgatttgt cattgacagg ataaataccg gcacctgcaa caggctcacc gctttgtttt
     1081 aggtaaatat ttcctttcaa cacacccatt tgtgcatagc aggaaatacc aattaagata
     1141 ttaacgaaaa ttaatagtat agattttttt gcgttattaa gatagcgcat attaatttct
     1201 gataattact tttccgttat tgatgatacc tgatttattt tcgatgctat aaaaatacaa
     1261 accatccaaa gaagaaacat ctacggtgct ttgttgtgag ttaagttgtg tttcttttac
     1321 tatacgtcct gcagtatcaa tcaacttaaa ttgataaaca ccggtatttt tatagttaaa
     1381 ccgaattttt tcttttatga tattgcccag aattgtcact tcgttttttg tatcaataga
     1441 atttactgaa gagggattaa aaaccatggc tatatcatca ataaaaatct gactgttgac
     1501 aataggtcct actcctttgc tggaggagag tatccaaata gagtttgaag gtgttgctgt
     1561 agaaagataa ttgacagcag cagcaaatcg tgtgtatgtg cttattgtgg cattaggtgt
     1621 ttcaaatctt acatagccaa tggtatctgt atttgtagca cctaacaatt gcagttcaac
     1681 aaaaccctga tctgttcctg ctggagtgta tttataccat cctgtaatac tatccggacg
     1741 accggtatag gcaataccac ctgatactcc ataaggcggt gttgtaatca atgttcctgt
     1801 gcttgcaata ccatttgcta ttacattaaa acccggaatt aatttggtgg ttaatttaat
     1861 tgcatagttt ccagtatgag catctgcacc tgaagcacgt gcacaagtta gtacacctat
     1921 gattcctgtg gttggattta gattattcca atcggcaggg tcaaaacggt tgccaacctg
     1981 attccagttt tcaaaacctg cattaggtgt tgcattttgt gccttaatgc ttatggcagt
     2041 tagaattaaa aatatggttg tataaagtct tttcattagt aattgagttt gcattaactg
     2101 ggcaaatata gcgcaatgaa tcaaaatttt gttttgattg taaattactt actcacagca
     2161 ggttgtagca gaaaaaacag attttgttct atacggagtt tctcctttaa gtttatgggt
     2221 cctaaaaaat caagattatg cttaaggtga ttgcccttca tacttgcatt aaatacaatg
     2281 tcacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatcaa gttgtgcaag
     2401 cgtattgttt tctaataaaa acttcacaac actaccaccc cccataccaa gaactaatac
     2461 tttttttttg tttttaatgt caagattaaa acgaagcaat gcttttataa aaactcggta
     2521 aagactacca taactgtaat tggcatgggc agtgttcaga accagtttgc cattttccaa
     2581 ggtagtctct aaaacactat tcacgtccga ttgatctttt tgtatgatga cgggatatag
     2641 aaagcttaac aaccattgaa aaaaagaata cttttttgcc atcatgcaaa gatagctacg
     2701 gagaatattt tatggaatgt aaatgggttt aattactgct tttttagaaa ctttaagtca
     2761 gaaagtaaaa tataatttag ttaattgttt tatttctttc ttacagtttc attgactgtt
     2821 gcaacaactt tgtcaacttg tatatcacct tcttcctgac ttaccaaaat cccatttggt
     2881 ttaaatgtgc tgatgatatt tgaatgtgaa aaatcaatag gtgaaatctt tttatatttc
     2941 attgaaagca cgtttgcaaa ttcttttgtt gcattttcat cagagcgtaa aaatgtccat
     3001 tgtggtcctg tcatttcgtt tttaatggaa aattctttca ggtgctccgg agtgtctgtt
     3061 tcaggatcaa tagaaactaa aacgaggttg attttatcaa tgtctttacg atcaaatttg
     3121 gattcaatcg ttttcatttt tgcaacaaga atcggacagg cagttttaca tgttgtataa
     3181 atcataacta ctaccaacgc cttgccttgt aagtcattta gctttattgt tttcccgtct
     3241 tgattttgcc agtcagaagt gagattaaaa attgagttgg atggaagttg ttcagttact
     3301 tctgtatcct gcttggattc tgttttttct ttgcaggaaa caagcattaa aagcgaaatg
     3361 caaaaaagtg aaatgagttt cataaaatga tagtgttatt gattaattaa tgttgtgtct
     3421 tttgcacaac ggaatcctaa atttccaata gtgtaatcag ctttgatgga tgtgcggagt
     3481 gcaaatctca tgtatgcagc ataattcata acatcagttg ccgatgttgc accgcctgca
     3541 cagaaaagat ttttatcatt atattctcca cttcgcgaat cgccagtagt aagtacagca
     3601 ttaaaatcat ctgtccattc ccaaatcaga tcaaacatat tgtaaatacc ccaatagttt
     3661 gggggacttt gttttactga tttgaactgt ctgttttttt gaaggtataa atcaatgatg
     3721 tcatctgagt attttgcttt acttcttgca ttggctgtat cgccatctgc cattgcaaca
     3781 aattcccatt catcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctccg
     3901 ggaatataaa ccatcttaac ggcactttgt atcctttgta atgtatcagt tttattgtcc
     3961 aaagaggtac tgttaacagt ttccttttcg gtttccttag aacatgatgc aaaaacaagt
     4021 ataaataaaa aaatgattaa gtgtttcacg ttacatttaa atgtttacaa aagcgggtta
     4081 ttcttttgtc aaattttttg cactgaatac tttctcgtta ggttcaccgg taactttaag
     4141 ttttccaata caacctttgt taaatgctct tgacagagag tggtcaacca gaacgtattc
     4201 gccaggaaca tcacatttaa attcaactat ggcagagccg cctgcaggaa tcattgttgt
     4261 ttgaatgttg tgcgaaactg tagatccacc ttcaagataa acattgtcaa aaatttctcc
     4321 aataacgtga aatgacgatg tcaaatttgg accaccatta ccaatgaaaa gtcttacagt
     4381 ttctccaact ttagcttcaa gcatattggc tccaagtaat gaacctttct ttccattaaa
     4441 taaaacataa tccggatttt cagcaactgc tttgtcattg tcaaactcca ttaaaccttg
     4501 tttaactact gatgctttag tatagaaatc accttgcata atgtagtatt ctctatcaac
     4561 gggctttaat ccaccggcag gttcaaccaa tataagtcca tacattccat tgccaatatg
     4621 cataggcacc gggggtgcag cacaatggta aacataaaga cctttattga ttgctttaaa
     4681 cgaaaatgaa gcttctttgc cgggaggcgc agtagttgca gcagcaccac cacctggtcc
     4741 tgttacagca tgcaagtcaa tgttatgtgg taaaatactg ctggcatcat ttttaagatt
     4801 taatgttact tcatcacctt ctcttactcg gataaaactt cccggtactg tgttattgaa
     4861 ggtccagaag gtataaggaa tactgtcggc aataatccct tctatctctg ttgttaccaa
     4921 atttacaatg agcttttttg cagctctggt accgatagga gccggaactt ttggtgcagc
     4981 tgtaacctct gctacttcgg gattaccttc agcaaccatt gccagtgctg cctcggcaga
     5041 acttgtttcc ggttttgttg tgcttgttga attacaacta agaagtgctg taattaataa
     5101 cattgttgtg taaattgtga aatgtttgtg tttcatttta ttattagttt aaaaatttgt
     5161 ttttaaaaag tgtggtgtta catttagcga aagccaaaac cattgctgat aatcattcgc
     5221 tcccggagta ttttttaaat acttcagagt tgttgttgct aaataaaagg aatatccacc
     5281 ggtaaaggtt acaaatgtat ttgttttaaa tgagaatgat aaatctagct cctgaccaag
     5341 attgctgttg tattttttat tgtagctatc actgatttta tttggagaaa taaactgatg
     5401 taatgctgca tttaatacaa gcttgccggt agttttatag ttaactttta aatagctgtc
     5461 tgatattcct gtgtttttat gtggattgcc ggcatagtaa tagtccatgt ttccataaaa
     5521 tttatgtcct gtatgaaagt acggattgaa tgcatggttg atcttatttg tttgtccaac
     5581 atcattgcca ctaacataat cacttcctaa accaagatta aacttgttgt taatttgaaa
     5641 tgctgcatta gctgtaatta aaaaggcatt tgtgtttatt gtttgcgaat tatcacagta
     5701 ttgataataa gctgcaatat gaatgtcagt tttgtttttc ttgaaatagt tgttcaagcc
     5761 aaaagtttga gtgaatattg tgttagtatc tgttgtacta taagttgctt gttggaatcc
     5821 aagattgttt gcaagcactg taaacctgaa atattttcct gcgggaatat ttgcccatat
     5881 tgtctgcatc cacttataag gaaatccatc ggttgttgaa tataaattac ctgatgggtt
     5941 attcaggtta ttcgcataca ttactttgta attctgattg aaggaagcat atccacgaag
     6001 ctcaaaagat tttctgtgaa agtcaattga aatagcatca tgtgcacgac ctccttgtgc
     6061 ccaatctaat tcaccaaaga aacgctcatc atctaaggta ataacctgtc ggccgatttt
     6121 aatatttaca cttggcgata ggttgatttt tgaccaggct tcaaacagag caaactgatt
     6181 gccgctgtat tctgcacctt gtaccatatt tgcctgaccc cagactccaa ccgtttgagg
     6241 agaaattttt atcgtaaaca aatctttgta agcgtaatca aaagttaaac gaattctttc
     6301 gctaaccaat gttgccggtt gttctttttt tgtgagtggc ctgaaggcac catcgcgtaa
     6361 ttcaaaacgt gttcttagct ggcctcttat gttaaaatta ttttctgatt ttagggtatc
     6421 ctgtgcttgt gaatcagtag gaaaaataaa gctgagaaac aggacaaaga ggccactatt
     6481 tattgcgttt tttactttat taagtgaaag cataaatagt attttaattc cggtgcaaag
     6541 gaagtatcag attgtaaatt gagtaatgat taaagtcatt gaatgattgt gattattata
     6601 atgagtaaaa atgacttaag tagcggtaag aatagaatgt ctgaaagatt atagctatat
     6661 attccgccaa tttcacttca gtagttatgt ttttttgttt taggcttatt caatggattt
     6721 tgtcaggttg atggttgttt agtattaatt atagaattga tttatttttt gaatttatgt
     6781 attgccatct ctgctataga aaatgtattt gctctttaag taactgattt ttgttgaatc
     6841 attacctttg tacaaaattt gcaatcatga atcttgaaca aaaaataatg gcagaattga
     6901 aaactgcaat gctcgctaaa aatgaggccg ctttacgtgc ccttcgtgct gttaaatctg
     6961 ctattatatt agcaaaaacc agtggctcaa atgttgaatt gtcggaagcc gatgaaatga
     7021 aattattgca aaagttggtt aaacagcgta aagaatcatt agatattttc acacaacaaa
     7081 accgttccga ccttgcaaaa aatgaagcag acgaaattgc tgtgattgag aagttcttgc
     7141 ccgcaatgat gagtgccgat gaagtaaaag aagtattgaa aggaatcatt gcctcaatgg
     7201 gagctacgca accatcagac atgggaaagg taatgggtgc tgcatctaaa caacttgcag
     7261 gtaaagcaga taataaaatg attagtga
//
LOCUS       DAOQNP010000107         7105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_4028_length_7105_cov_9.605248, whole genome shotgun sequence.
ACCESSION   DAOQNP010000107 DAOQNP010000000
VERSION     DAOQNP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_7105_cov_9.605248"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1120)
                     /locus_tag="PKY09_11515"
     CDS             complement(167..1120)
                     /locus_tag="PKY09_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNB13525.1"
                     /translation="MRTAVLIATIFTTLNISCATGQKNSAPNDTDMNNQLKNGTNIFF
                     ENKTINADLNFTSLLQPVVESNGINRCYINSAITFKQCTFKGKLTGYVTDESGKSTMT
                     YFQKSVAFIDCIFEDDVNLRGAIFSAPANFQGCSFVKKADFQESHFYSDAFFNETKYR
                     EEAYFQNAVFAKKLVFMNARFESNVYFQNATFYFDSQFSNAEFQKYADFTVCTFQMGV
                     FFNYCKFQQQAIFNSSVFGKRFEMLKITANKVELRKCYFGFVPKLMQSDFSELLDISG
                     SHFAEGIPVLKDITTKVLLSSEITSCNKNYTSEEFLKASGR"
     gene            complement(1223..2935)
                     /locus_tag="PKY09_11520"
     CDS             complement(1223..2935)
                     /locus_tag="PKY09_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNB13526.1"
                     /translation="MKWIKRLFLVSLALSLTLSACKKEKDDSPSPTPPTPIPPTPPGN
                     TPKNLGWNGTDNPNSVPTSVTNTPIYNSGTLPQSVSLLPQFPPIGDQGQYGTCVAWAA
                     GYNMKTVIDAIQQNLTANDLANTNKQTSPKDLFLAIPDAKKGQNCNGTNFSDAMDLLL
                     NRGAATMQTVPYTNLGNCASAGVQSNWTQEANQRKIKNYRKITASIQSVKEQLANKIP
                     VVIGAKLSDNFMTWNSDAVYNSNSTYNQVGQHSYHALIISGYDDSKGANGAFKVVNSW
                     GTNWGDIGYIWIDYNFLLNEFVFDQNFYTATTDGGTNPPSPSPTPSGGVDLASWVFGD
                     ETTYGSTQTQYYNERVVYWNLYNIGASAAASSTNWSLYYIYFNAYDANDYGVLFYDEF
                     NTSIAQNTYQVDPNDPNHIILNIDIPANNDLGNVGFGTQQLYQVYYTPSSLNGEYYIV
                     MVADAEGAFTEDNEQNNFFYTTDQYPKFFQNGYSGRVTAQMQSTNATNFVEYKFINKL
                     QPSKHNLTSNIYNSVVTKQMPNAYSSEEIGQMLKIKKRTGDLNRKLAEFKMRQKGNGG
                     FGTK"
     gene            3090..4277
                     /locus_tag="PKY09_11525"
     CDS             3090..4277
                     /locus_tag="PKY09_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNB13527.1"
                     /translation="MKINKIKQLAEAAFNTVLADRRHLHKNPELSFKEFQTQQFVWGR
                     LDEIGITNKVKIAETGIVAMIEGKNPSAKIIALRADMDALPIQETNKTDYVSQNAGVM
                     HACGHDVHTSSLLGVASVLQQFKNEFSGTVKLLFQPGEEKLPGGASMMIKEGVLKNPS
                     VSGILGQHVMPLIETGKVGFRSGVYMASTDEIYVTIKGKGGHGAIPHQNIDPVLIASH
                     IIVALQQIVSRNANPIMPSVLSFGKVVANGATNVIPDSVYLEGTFRTLDERWRNDAHA
                     KMIKMAEAIAEGMGGKCEFKIVRGYPALVNNPELTASARKNAVDYLGEENVLDLDIWM
                     AAEDFAFYSQETNACFYRLGTRNEAKGITSSVHTSTFDIDENALKTGVGLMTYLALCE
                     LKQ"
     gene            complement(4896..6572)
                     /locus_tag="PKY09_11530"
     CDS             complement(4896..6572)
                     /locus_tag="PKY09_11530"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNB13528.1"
                     /translation="MAHTDSKTTLCFDFEDSIQDCLSPANTTILKTNYRRYFDTIVEK
                     CNTEIGTYTIGIRINAVDSPEYVLDLEIVAKLKQPITIFAPKIDSSIQILKLHDDLKK
                     VGAGNNEIIPVIETKKGLDNLAEILKNSSTIISGIALGHCDYNLDTGNYPFFHQDSRE
                     YWTWITKIAELTKPYNLCVVNSPFLQLDNNEFFHYILYMLYAIGGNKIGQITLTQKQT
                     SICNFFPDNIKNYTLPKLFNRLDQRVPLLYAEKFIASFEKSNNLKGFSIDNDRTILSP
                     QEYFASKAFLHKIDFKEINFTFVGGCFPVQGDLPFENLFHQLLKQKLENSREIKFNVN
                     IIRYERFRNCRNKIEEYKQNQTIDILVFSIRPEPFLRLVKLYYKFMDSSTGRKKWSLN
                     LPIFNNVNPEKHDLLSFDARYNLSTFHDSSLIHKTLINLNYIFGLLLGNANYSQKLYL
                     DLVNEVIEYSRMNNIKLFILGPPIRANTRIERLLSKRLDKFIRKAPTIVQDNFISGSE
                     PIKNGEYLFKNGIYANEKYHELIAERMLEKIQPTIDKIAAVNRVGGPTRI"
BASE COUNT         2213 a         1157 c         1332 g         2403 t
ORIGIN      
        1 tggtaaggaa gtattttcct ttgttgtaga attgtttttt catgttattg aattttagtc
       61 caaagaaaag acaaaggctt ttagctgcca aacggatttc cttattttct tacccgttta
      121 aaatcatatt atttagtaga ataacacata aaatcaggta aaaaacttac ctgccagaag
      181 cttttaaaaa ttcttctgag gtgtaatttt tattacagga agtaatttct gatgataaaa
      241 gcactttggt agtgatatct ttcagtacag gtattccctc agcaaaatga ctgccactaa
      301 tgtctaataa ttctgaaaaa tcgctttgca ttaatttggg tacaaatcca aaataacact
      361 tgcgaagttc aactttgttg gcagttattt tcaacatttc aaatcttttg ccaaatacac
      421 tactgttaaa aatagcctgt tgttgaaatt tacaataatt gaagaatacg cccatctgaa
      481 atgtacaaac agtaaagtct gcatatttct gaaactctgc attgctgaat tgtgaatcaa
      541 aataaaatgt tgcattttga aaatatacat ttgattcaaa ccttgcattc ataaagacca
      601 attttttagc aaatacagca ttttgaaaat aagcttcttc gcgatatttt gtttcattaa
      661 aaaaagcatc agaataaaaa tgagattcct gaaagtcagc tttttttaca aagctacatc
      721 cttgaaaatt tgccggtgca gaaaatattg ctcctcgtaa atttacatca tcttcaaaaa
      781 tacaatcaat aaatgctaca cttttttgaa agtacgtcat agtagatttt cccgattcat
      841 cggtaacata acctgtaagt tttcctttaa atgtacattg tttgaaggta atggcagaat
      901 tgatataaca tcggtttata ccattgcttt caacaacagg ctgtagcaga cttgtaaaat
      961 ttaaatcagc attaatggtt ttattttcaa aaaaaatatt agttccgttt tttagctgat
     1021 tgttcatgtc ggtatcgttt ggtgcactgt ttttctgacc tgtagcacag gagatattca
     1081 gtgttgtaaa aattgtagct ataagaactg ctgtacgcat aatatttttt tacaagttta
     1141 aaaatcattt ggtgaaaaag aaaaaaatcc cggcagaaat tttcttgccg ggatgatatt
     1201 gtttgcggtt taaatgttaa gcttatttgg tgccaaaacc accgttgcct ttttgtctca
     1261 tcttaaattc tgcaagttta cggttaaggt caccggttct tttcttaatc ttcaacattt
     1321 gcccgatttc ttcagatgaa taggcattgg gcatttgttt ggtaacaaca gaattataaa
     1381 tgttagaagt cagattgtgt tttgaaggtt gaagtttatt gatgaactta tattctacaa
     1441 agtttgttgc attggttgat tgcatttgtg ctgttacacg tccgctatat ccgttttgga
     1501 agaattttgg atactgatcg gttgtgtaaa agaaattgtt ttgctcgtta tcttctgtaa
     1561 acgccccttc tgcatcggca accattacaa tataatattc tccatttaat gatgacggtg
     1621 tgtaataaac ctgatacaat tgttgtgttc cgaaaccaac attacccagg tcgttatttg
     1681 caggtatatc aatattcaga ataatatgat tcggatcgtt tgggtcaacc tgataagtgt
     1741 tttgtgcaat agaagtgttg aattcatcat aaaataaaac accataatca ttggcatcat
     1801 aagcattaaa gtatatgtag tacaaactcc aattagttga actagctgct gctgaagcgc
     1861 caatattata aagattccag taaacaacac gctcattgta atattgtgtt tgtgtagatc
     1921 cataagtagt ttcatcacca aacacccaag aggcaaggtc aacgcctcct gatggtgttg
     1981 gagaaggtga aggagggttt gtgcctccat cggttgtggc agtataaaag ttttgatcaa
     2041 atacgaattc attcaataga aaattgtagt ctatccaaat atatccgata tcaccccaat
     2101 tggtgcccca tgaatttaca actttgaaag caccatttgc gcctttacta tcatcataac
     2161 cggagattat cagcgcatga tatgagtgct gtcctacctg attgtaagta gaatttgaat
     2221 tgtaaactgc atcactattc caagtcataa aattgtcgga aagtttagct ccgataacta
     2281 caggtatttt attggctaat tgttctttaa cactctgaat ggatgccgtt atttttctgt
     2341 aattttttat tttacgttga ttggcttcct gtgtccagtt gctttgtact cctgcgctgg
     2401 cacaatttcc aagattagta tatggaacag tttgcatcgt tgcagctcca cgattcaata
     2461 acaaatccat tgcatcagaa aagtttgtac cattgcaatt ttgtcctttt tttgcatccg
     2521 gaatagcaag aaaaagatct ttagggctgg tttgcttgtt ggtatttgct aagtcgtttg
     2581 cagtaagatt ttgctgtata gcatctataa cggttttcat attataacca gctgcccatg
     2641 ctacacaggt gccatattgc ccctgatcgc caatgggcgg aaactgtggt aagaggctta
     2701 ccgattgcgg taatgttccg ctgttataaa taggcgtatt ggtaactgaa gtaggtactg
     2761 aattagggtt gtcggttcca ttccacccta aattttttgg ggtattacct ggtggtgtag
     2821 gaggaatagg agttggaggt gttggtgacg ggctgtcatc tttttctttc ttacaggcac
     2881 taagggttaa agataatgcc agtgaaacta aaaataatcg cttaatccat ttcatttctt
     2941 atgatattaa gttggtgaat gctacaaaac tgattataaa aatttttatt gtcaatcacc
     3001 aaaactggtg atttttatgg ctgaaatcaa tacttaattt gatgtattaa atcacaagtt
     3061 attgaacaat aaagttttaa ttttgacgca tgaaaatcaa taaaattaag caacttgcag
     3121 aagctgcatt taacacagta cttgcagaca gacggcattt gcataaaaat ccggaattat
     3181 cctttaaaga atttcagaca caacaatttg tttgggggag gttggatgaa ataggaataa
     3241 ctaataaagt gaaaattgca gaaaccggca ttgttgctat gattgaaggt aaaaatcctt
     3301 ctgccaaaat tattgcactg cgtgctgata tggatgcact tcccattcag gaaaccaata
     3361 aaacagatta tgtttcacaa aatgcaggtg tcatgcatgc atgtggtcat gatgtccaca
     3421 cttctagctt attgggtgtt gcttcggttt tacagcaatt taaaaatgag ttttcaggaa
     3481 ctgtaaaatt attgtttcaa cctggtgaag aaaaactccc cggtggtgca tcaatgatga
     3541 taaaagaagg tgtattaaaa aatccttcgg tttcaggaat tttaggacaa catgtaatgc
     3601 cgttgattga aaccggcaaa gtgggtttca gaagcggtgt gtatatggcc agtacggatg
     3661 aaatatatgt aaccataaaa ggaaaaggag gtcatggagc tataccacat cagaatattg
     3721 atccggtatt gatagcatca catatcattg tcgcattgca acagattgta agtagaaatg
     3781 caaaccccat tatgccaagt gttttgtcat tcggtaaagt tgttgccaac ggagcaacca
     3841 atgtaattcc cgacagtgtt tatcttgaag gaactttcag aacattggat gagagatggc
     3901 gtaatgatgc ccatgcaaaa atgataaaaa tggcagaagc tatagccgaa ggaatgggag
     3961 ggaagtgtga gtttaaaatt gtgcggggat atcctgcatt ggttaacaat ccggaactta
     4021 cagcatctgc cagaaaaaat gctgttgatt atctgggaga agagaacgtg cttgatcttg
     4081 acatctggat ggcggcagaa gattttgcat tttattcgca ggagaccaat gcttgctttt
     4141 accgtttggg gacaagaaat gaagccaagg gtattacttc atcggtacat acatcaacat
     4201 ttgatattga cgagaacgca ttgaaaacag gtgtaggtct aatgacttat ctggcacttt
     4261 gcgaattgaa gcagtaacaa agggttaata atagcgcttc ttcaaagttg gatgctcaat
     4321 aaaagattat ttaaaaccgt gtaggatagt aatgttatct atatgtaata agtttcctgt
     4381 ttataatatt tccgatgttg tgaatagaaa tagtgttcat accgttttca aatttattca
     4441 ctactcgctt gcgattacta ttgctgaaca catgatgtat tttcagacgt tgaaagtcaa
     4501 caacaaaaga atttagtgtg atctttgttc cacaaacgaa tgcttgtgcg ataattttag
     4561 caataatatt ttaagagaaa ctgatttttt tcgcagcatg acggtttatg tatttgcgaa
     4621 gtataataat tttgtcactg aatcttaatc gaagaatccc gatagatatt ggggttgaac
     4681 cttgctaaaa atcaaaatct tggttcaacc atcactttgg caaggttaaa tcaatgagga
     4741 tacgtaagtt tgttggacgt ttcacaagac taaacccatc tacatgtgaa ccgtctgcca
     4801 tgcgagatgg tgtttgagta acttattatt tttctaatgg aattcatgat atcgctttgc
     4861 ggtgtgagat accgatagct ttcggtagca cttcatcaga ttctggtagg gccacctact
     4921 cgattaactg ctgcaatttt gtcaattgtc ggttgtattt tctctaacat tctttcggca
     4981 atcaattcgt gatatttttc atttgcataa attccatttt taaatagata ctctccattt
     5041 ttaatgggct cagaaccgct aataaaattg tcttgtacaa tagtcggagc tttccttata
     5101 aacttatcaa gtctttttga taaaagtcgc tctattcttg tatttgctct aattggtggt
     5161 ccgagaataa atagtttaat gttattcatt ctgctgtatt caattacctc attcacgagg
     5221 tctaaatata acttttgaga ataattagca ttgccaagta ataatccaaa aatgtagttt
     5281 aggtttatca atgttttatg aattaatgag ctatcatgaa acgtggaaag attatatctt
     5341 gcatcaaaag aaagcaaatc atgtttttcc ggattcacat tattaaatat cgggagattt
     5401 aatgaccatt tttttctacc agtagagcta tccataaatt tgtagtacaa ttttaccagt
     5461 cgtagaaatg gctctggtct tatagagaat acaagaatgt ctatggtttg attttgttta
     5521 tattcttcga ttttgtttct gcaatttcta aagcgttcat atcggataat gtttacatta
     5581 aattttattt ctcgactgtt ttccaatttt tgcttgagta attgatggaa aagattttca
     5641 aaaggtaaat caccttgaac gggaaaacaa ccgccaacaa atgtaaaatt gatctcctta
     5701 aaatctattt tgtgcaggaa ggctttcgat gcaaaatatt cttgtggaga taaaatcgtt
     5761 ctatcattat caattgaaaa acctttaagg ttgttacttt tctcaaatga tgcaatgaat
     5821 ttttcagcat ataatagggg aaccctttgg tcaagtcggt taaataattt tggaagcgta
     5881 taatttttta tattgtctgg aaagaagtta caaatacttg tttgcttttg tgtcaaggta
     5941 atttgaccaa ttttgtttcc gccaattgca tatagcatat atagaatata gtggaaaaac
     6001 tcattattat ccagttgaag gaatggagag tttacaacac acaaattata cggcttagta
     6061 agctctgcta tttttgttat ccaagtccaa tactctcttg aatcttgatg aaaaaacgga
     6121 taattgccag tgtcaagatt gtaatcacag tgccccaaag caataccact aattatagtt
     6181 gaactatttt ttagaatttc tgctaaattg tccaaccctt ttttagtttc tataacaggg
     6241 attatttcat tattgcctgc tccgactttt tttagatcat catgcaactt caaaatttga
     6301 atagaactgt ctattttggg agcaaagatt gtgattggct gctttagttt agctacaatt
     6361 tccaggtcta aaacatattc aggagaatcg actgcgttta ttctaatacc aattgtatag
     6421 gtaccaattt cagtgttaca tttttcaacg attgtgtcga aatatcttct atagtttgtt
     6481 ttaagaattg ttgtgtttgc gggagatagg caatcttgga tactgtcctc aaagtcaaaa
     6541 catagcgttg ttttactgtc ggtatgagcc aatagcgagt ctaatgacgt actttcatta
     6601 tattttatgt aatggtatgc tctcatcgga tatgttgttg cagcttacgt cagaattccg
     6661 agcaatcagg acaagttgtg aaaaagtaat ttaaaagcat ttacaaatat aaattaaatt
     6721 caatagtttt aaggagcaaa acagcaatta aaaatgccac aaatacaagg gatcaaccgc
     6781 gaacaaattg cattttcaaa taacctaaaa cgagcactaa agtccaatct cttggttcaa
     6841 ccatcacttt ggtaaggtta aagcaacgag gatacgaaag tttgttggag gtttacacaa
     6901 cactaaaccc atctatctgt gaaccgctaa gtatgcaact gagattgaat gattcaatat
     6961 catttaatcg aaagttggga gcgtagctcg cgtacgctta gtggtgtgag aggtgcactc
     7021 cgtcagtctc tggcggagcc gcctactcga ttagctgctg tggtttttct tctttgtgga
     7081 tttcgctttc ttattgaagt caaga
//
LOCUS       DAOQNP010000108         6672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_4406_length_6672_cov_8.127853, whole genome shotgun sequence.
ACCESSION   DAOQNP010000108 DAOQNP010000000
VERSION     DAOQNP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_6672_cov_8.127853"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1349)
                     /gene="tuf"
                     /locus_tag="PKY09_11535"
     CDS             complement(162..1349)
                     /gene="tuf"
                     /locus_tag="PKY09_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNB13529.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLANKGLAEL
                     RSFDSIDNAPEEKERGITINTSHVEYQTENRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPKIVVFMNKVDMVDDPELLDLVEMEIRELLSF
                     YEFDGNNTPIIRGSALGGLNADAKWVDKIMELMAAVDTYIPIPPRQVDLPFLMPVEDV
                     FSITGRGTVATGRIERGVINSNDNVEIIGMQDKKLTSTVTGVEMFRKILDRGEAGDNV
                     GLLLRGIEKTDIRRGMVVAKPGSITPHTEFKAEIYVLKKEEGGRHTPFHNKYRPQFYL
                     RTTDVTGEIVLPEGREMVMPGDNVTITVKLIVPVAMDKGLRFAIREGGRTVGAGQVTE
                     IIK"
     gene            complement(1431..1772)
                     /locus_tag="PKY09_11540"
     CDS             complement(1431..1772)
                     /locus_tag="PKY09_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13530.1"
                     /translation="MSSILINGSKNFERCMEHSNEIKRVVSSIPIAIGINGSKNFERC
                     MEHSNEIKRVVSSIPITIGINGSKKFERCMEHPNEIKRVVSSIPITIGITVQIVRIIE
                     IEKKQTHINQL"
     gene            complement(1748..1819)
                     /locus_tag="PKY09_11545"
     tRNA            complement(1748..1819)
                     /locus_tag="PKY09_11545"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1785..1787),aa:Thr,seq:ggt)
     gene            complement(1957..2029)
                     /locus_tag="PKY09_11550"
     tRNA            complement(1957..2029)
                     /locus_tag="PKY09_11550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1994..1996),aa:Gly,seq:tcc)
     gene            complement(2052..2137)
                     /locus_tag="PKY09_11555"
     tRNA            complement(2052..2137)
                     /locus_tag="PKY09_11555"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2101..2103),aa:Tyr,seq:gta)
     gene            complement(2199..2272)
                     /locus_tag="PKY09_11560"
     tRNA            complement(2199..2272)
                     /locus_tag="PKY09_11560"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2236..2238),aa:Thr,seq:tgt)
     gene            complement(2625..3101)
                     /gene="purE"
                     /locus_tag="PKY09_11565"
     CDS             complement(2625..3101)
                     /gene="purE"
                     /locus_tag="PKY09_11565"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNB13531.1"
                     /translation="MKVAIIMGSKSDLEVMQQAAQVLSDFQIEHHIQIVSAHRTPELM
                     VQFAKEAYEKDYRVIIAGAGGAAHLPGMVASLTALPVIGVPVKSSNSIDGWDSILSIL
                     QMPNGVPVATVALNAAQNAGLLAVQILATSDKAIYKKLEAYKQSLRDKVIKSNEGL"
     gene            3211..4419
                     /locus_tag="PKY09_11570"
     CDS             3211..4419
                     /locus_tag="PKY09_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNB13532.1"
                     /translation="MLQHVKSLFSGAYSGISRDVWLLSAVMLINRSGTMVIFFLSVYL
                     TEKLGFSVQQTGVVMACFGAGSFTGAFTGGKLTDIIGYRPVMLISLFTGGTLFMLIPF
                     IHSYLYLCVSFFFLSIFSESYRPANMTAIAFYSKPGNYTRSISINRLAINLGFSVGPV
                     LGGFLASISYDYLFYTNGCFCIAAATFTLFMLHNKKVHHVHGQSQKQQIASPYKDKIY
                     LYFIVAVFLYALCFFQFFITMPLFYKDIHHLSERQIGLLMMFNGLIVAVVEMILVYRL
                     EKRWNVYRTISIGGLLLAATYLCLPFIGGFAAFTLIIVLVSFSEMLAMPFMNSFMNLK
                     SSIASRGQYAALYSMAWSSAQTALPILATQTIANFGYNTLWMLLAFILIIMTYIIRVV
                     SQKMRAAAAN"
     gene            complement(4416..5075)
                     /locus_tag="PKY09_11575"
     CDS             complement(4416..5075)
                     /locus_tag="PKY09_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNB13533.1"
                     /translation="MISKQTLSFLRALAVNNNREWFVANHHKYESAKEDFNNCLKQTL
                     SELTKYDASLKGLEPKDCVFRIYRDIRFSPDKTPYKTNLGAAINEGGKKKETAGAYLH
                     IQPGNKSFFAGGRWMPDKETLRKIRQEIDYNTDEFKTILNNKNFKKYFGTLEDIALKK
                     APKDYPKDHPEIALLKYTSYIASMPLADSLITSDKGVKEIGKVYKALMPLLHFLNRAL
                     D"
     gene            complement(5104..>6672)
                     /locus_tag="PKY09_11580"
     CDS             complement(5104..>6672)
                     /locus_tag="PKY09_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13534.1"
                     /translation="ITGIAGSTITISTPTIGAMTAATQVRFNVNGALSPNSNDGNCTT
                     TQRPNLTFKYKRKYTTFMAYVGGHWNNSGTFTAGQSDFTFNGMNPQNIDGANNTTFMA
                     MTINKPAPANPVRLYKDVTVTDTLHLTQGRLQLNGSPTVIRSLSLMSNTGSTAALTRN
                     TGVLIAESSPPNYGNFRWKIGTPAAYPTTFTFPFANSAGVYIPVDYKVNNGDADVTMT
                     TYSTIPANTPIPTGVSDIYMNTWFAPPFTDNSPYMVDRFWQINDAGTVTSRDLTLNWV
                     SSENAAAGSSPYRAQVYHPSTNWGYPFIIGQTNPTTTSVSIPAATYSNTWAVVRELQP
                     LPVELLSFEAKPYKDQEVVCNWTTATELNNDFFTVERSSDGRNFEKVGTVKGAGNSTQ
                     QHDYKFIDTKPYRGVSFYRLKQTDFNGKSETFEKVAVNLSLPATPISVYPVPANEYVV
                     FSVDRNQNLENAVLYVYDFTGRALIQKKVAELKGTGTNIFILPVNTLINGLYHFDLKV
                     EGTSLGNGKFIVEK"
BASE COUNT         1827 a         1386 c         1276 g         2183 t
ORIGIN      
        1 gattcgaact cccatcaacg gttttggaga ccgctattct acccttgaac tacactcgta
       61 atttatgata gataattaaa ccgttctcct gcgaaagccg ttgcttccgt atattggttt
      121 tcgccagccc cgatattacg gggctgacaa ttccaataca attatttaat tatctcagta
      181 acctgaccgg cacctactgt acgtccacct tcgcggatag cgaaacgaag acctttgtcc
      241 attgccacag gaacaattaa ttttactgtg atggtaacgt tatcacctgg cataaccatt
      301 tcacgtcctt ctggaagtac gatttcaccg gttacatcag ttgtacgtaa gtagaactgt
      361 ggacggtatt tgttatggaa aggtgtgtga cgtccacctt cttctttctt aagaacgtaa
      421 atttctgctt taaactcagt gtgtggagta attgagcccg gcttagcaac taccatacct
      481 ctacggatat cagttttttc tataccacgc aacaacaaac caacgttatc acctgcttca
      541 cctctgtcaa gaattttgcg gaacatttcc acacctgtaa cagttgaagt aagtttttta
      601 tcttgcatac cgataatttc aacgttatcg ttagagttga taacacctct ttcgatacga
      661 cctgtagcaa ctgttccgcg acctgtaatt gagaacacgt cttcaaccgg cataaggaaa
      721 ggaaggtcaa cctgacgagg aggaattgga atgtaagtat caactgctgc catcagttcc
      781 ataattttat ctacccattt agcatcagca ttcaatccac ctaatgcaga accacgaatg
      841 attggtgtgt tgttaccatc aaattcatag aacgacaaaa gctcgcgtat ttccatttca
      901 acaaggtcta acaattctgg atcgtcaacc atgtcaactt tattcataaa aacaacaatc
      961 ttaggtacac ctacctgacg tgcaagcagg atgtgctcgc gtgtctgagg cattggacca
     1021 tctgttgcag caactacaag tatagcacca tccatctgtg cagcacctgt taccatgttc
     1081 tttacatagt cagcgtgacc tggacagtca acgtgtgcat agtgacggtt ttctgtttgg
     1141 tactcaacgt gcgatgtatt gatagtaata ccgcgttctt tttcttcagg agcgttgtcg
     1201 atagaatcga atgaacgcaa ttctgccaga cctttatttg ccaacacagt tgtgattgca
     1261 gcggtcaagg tggtctttcc gtggtcaacg tgaccaatag ttccaatatt tacgtgtggt
     1321 ttggaacgat caaatttttc tttagccatt tttttattta ataaacaggt tatttaaata
     1381 attgatttgt gagatatata taattaataa ttgttaactc taaattcatt tcaaagttga
     1441 ttaatgtgcg tctgtttttt ttctatctct ataattcgta ctatttgaac cgttatcccg
     1501 atagttatcg ggattgaact cacgaccctt ttaatttcat tggggtgctc catacaacgt
     1561 tcaaatttct ttgagccgtt gatcccgata gttatcggga ttgaactcac gaccctttta
     1621 atttcattgg agtgctccat acaacgttca aaattttttg agccgttgat cccgatagct
     1681 atcgggattg aactcacgac ccttttaatt tcattggagt gctccataca acgttcaaaa
     1741 ttttttgagc cgttgatgag aattgaactc acgacctctt ccttaccaag gaagtgctct
     1801 accactgagc tacaacggct ttttaattcc ttgtacataa ttcccagtac tcggggtgag
     1861 cccttataac ttcaattcgc aagtttcaac gctgtcccaa accttttgag cggaagacgg
     1921 tcccgataac tatcgggatc gaacccaaac cttttgagcg gaagacgggg ctcgaacccg
     1981 ccacctatag cttggaaggc tatcgctcta ccaaatgagc tacttccgct tgtttttttt
     2041 accggcatca ttggtgggga gtggtggatt cgaaccaccg aagtcataag acaacagatt
     2101 tacagtctgt cccatttggc cactctggta actccccgat aatgttcagt tttaccttag
     2161 ccaattgttg acaaactgaa aatatataaa taaaaaagag ccgatggagg gattcgaacc
     2221 cccgacccac tgattacaaa tcagtagctc tggccaactg agctacatcg gcctcttaaa
     2281 tatcaatgat tcccaaacaa attactactt taccaatctg cttcagaagt tattacgcca
     2341 ttaatccacg atgtcaatga acacacttta cccttcaaaa aagggaacgc aaaaatagaa
     2401 aaggattttt tattagcaaa tggttattga aaatattttt aattgtcctt taaacaattc
     2461 tatcacttca ttcaacatat tgataatgca ttagttagca aaacaatatt tcagcaattt
     2521 taaaatttat caccaataaa aaaaacagct aaaggcctat gtggttgtat attagtggcc
     2581 ggaggctgca ttgtagctcc ctgtcatcaa aatttaatta aaaatcacaa cccttcgttg
     2641 gacttgataa ccttatctct taacgactgc ttataggctt caagtttttt gtatattgcc
     2701 ttatcggatg tggctaaaat ctgaacggca agtaatccgg cattctgtgc cgcattcaag
     2761 gcaacggtag ccacaggcac tccatttggc atttgtaaaa tagaaagaat agagtcccaa
     2821 ccgtcaatac tattgctcga ttttacagga acaccaatta caggaagtgc agttagtgat
     2881 gcaaccatac ccggaagatg ggctgctccc cccgccccgg caatgattac ccggtaatct
     2941 ttttcatagg cttctttggc aaactgtacc atcagctccg gtgtgcgatg tgcagaaaca
     3001 atctgaatat gatgttctat ctgaaaatca gaaagtacct gtgccgcctg ttgcatgact
     3061 tcaaggtctg atttgctacc cataataatt gctaccttca taataaaccg attgtattgg
     3121 aatgcaacaa aattaccgca taaatcaaaa cagtgcataa gtatttttat tcttcttgtt
     3181 ttgcaaggca acttatttca attttgctgc atgttgcaac atgttaaatc acttttttca
     3241 ggcgcctaca gtggcatttc gcgtgatgtg tggttacttt ctgctgtgat gctgattaac
     3301 agaagcggca ctatggtaat ctttttccta tcggtgtatc tgacagaaaa attaggcttc
     3361 tccgtgcaac aaaccggtgt ggtgatggct tgctttggtg caggaagttt taccggtgct
     3421 tttaccggag gtaaactaac tgatattatt ggttaccgtc ctgtgatgct gattagttta
     3481 tttacaggtg gtacgctgtt tatgctcata ccttttattc actcctattt atatctatgt
     3541 gtttcattct ttttccttag tatttttagt gaatcatacc gtccggctaa catgacagcc
     3601 attgcctttt acagcaagcc cggaaattac acacgctcta tttcaatcaa ccgtcttgca
     3661 ataaatcttg gcttctctgt cggacctgtg cttggcggct tcttagcttc tataagctat
     3721 gattacttat tttacaccaa cggatgtttt tgtattgcag cggcaacatt tacactgttt
     3781 atgttgcaca ataaaaaagt gcatcatgta cacgggcaaa gccaaaagca acaaattgct
     3841 tctccttaca aggacaagat ttatctttat tttattgttg cagtttttct ttatgcactt
     3901 tgtttctttc agttttttat aaccatgcct ttgttttata aggacattca tcacctgagc
     3961 gaaagacaaa taggtttact gatgatgttt aatggattaa tagttgcagt tgtagaaatg
     4021 attttggttt acagacttga gaaacgatgg aacgtttacc gtaccatttc tattggcgga
     4081 ctattacttg ctgctaccta cttatgtctt ccctttattg gtggttttgc agcattcaca
     4141 ttaattatag tattggtatc attttcagaa atgctggcta tgccattcat gaattctttt
     4201 atgaatctaa aaagctctat tgccagtcgc ggacaatatg cagcattgta ttctatggca
     4261 tggagttcgg cacaaacagc cttacctatt cttgccacac aaactattgc caacttcggc
     4321 tacaacacgc tttggatgtt attggcattt attctcatca tcatgaccta tattatcaga
     4381 gttgtttctc aaaaaatgag agctgctgct gcaaactaat ctaatgccct gttcaggaaa
     4441 tgcaggagtg gcatcaatgc tttataaact ttaccaattt cttttactcc cttatctgaa
     4501 gtgattaatg agtctgccaa tggcatggag gcaatgtagc tggtatattt aagcaacgct
     4561 atttccggat ggtcttttgg atagtcttta ggtgcttttt taagcgctat atcttccaat
     4621 gtaccaaaat actttttaaa gtttttgtta ttcaaaatag ttttaaactc atcggtattg
     4681 taatctattt cctgtctgat ttttcgtaaa gtttccttat cgggcatcca ccgtcctccg
     4741 gcaaaaaaag acttgttgcc cggctgaata tgcagataag cacctgctgt ttcttttttt
     4801 ttgcctcctt cattgatagc tgcaccaagg tttgttttgt aaggtgtttt atcgggcgaa
     4861 aaacgaatat cacgatatat tctgaaaaca cagtctttgg gttcaagccc tttgagcgaa
     4921 gcatcgtact tcgtcaattc cgagagcgtt tgtttcaggc agttattaaa atcttctttt
     4981 gcactttcat acttatggtg gtttgcaaca aaccattcgc ggttgttatt aacggcaagt
     5041 gcccttaaga atgaaagcgt ctgtttactt atcatactta agggcacttt gtcttttaaa
     5101 aaattatttc tctacaataa atttaccgtt tcctaatgaa gtgccttcta cttttaaatc
     5161 aaaatgatac aatccattaa ttaaagtgtt tacaggcagg ataaatatat tggttcctgt
     5221 gcctttaagt tctgcaactt ttttctgaat caatgcacgt cctgtaaagt cataaacata
     5281 caatactgca ttttcgagat tctgatttct gtcaacactg aatactacat attcgttggc
     5341 aggaacagga taaacactaa tcggtgtggc aggtaaactt aagtttacag ctactttctc
     5401 gaacgtctcg gacttgccgt taaaatcggt ctgtttcaat ctgtagaacg aaactccgcg
     5461 ataaggtttg gtatctataa atttatagtc gtgttgttgt gttgaattac ctgcaccctt
     5521 tacagttcct actttttcga aattacggcc atcgctgctg cgttcaacag taaagaagtc
     5581 gttattgagt tctgtagctg ttgtccagtt acaaaccact tcctgatctt tataaggctt
     5641 agcttcaaac gaaagcaact ccacaggcag tggttgcaat tctctcacaa cagcccatgt
     5701 attgctataa gtggctgccg gaatggatac tgatgttgtt gtaggatttg tttgaccgat
     5761 aatgaacgga tagccccagt tagtacttgg gtgatatacc tgtgcacgat aaggagatga
     5821 acctgctgct gcattttctg acgataccca gttaagcgtt aagtcgcgac tcgttactgt
     5881 acctgcatca ttaatctgcc agaaacggtc aaccatataa ggactgttgt ctgtaaatgg
     5941 tggagcaaac caggtgttca tataaatatc tgaaacgcct gtcggtattg gtgtatttgc
     6001 cggtattgtt gaataagttg tcatggtaac atctgcatca ccattgttca ctttatagtc
     6061 aacaggaata taaactcctg ccgagttggc aaacgggaat gtaaaagttg ttggataagc
     6121 tgcaggtgtt cctatcttcc atctgaaatt accatagttt ggcggactgg attctgctat
     6181 caatacgcct gtgtttcttg tcaatgctgc ggtggaaccg gtgttactca tcaggcttaa
     6241 tgagcggata acagttggac ttccattaag ctgcagtctg ccttgtgtta agtgcagtgt
     6301 gtctgtaacc gtaacatctt tataaagcct aacaggattt gccggtgcag gtttattgat
     6361 ggtcattgcc atgaatgtag tattattggc accatcaata ttttgtggat tcataccatt
     6421 aaaagtaaaa tcgctttgac cagcagtgaa ggtaccggag ttgttccagt gaccacctac
     6481 ataagccatg aaggtggtgt atttacgttt gtatttaaat gtaaggttgg ggcgttgtgt
     6541 tgtagtacag ttaccatcat ttgaatttgg actcaatgca ccgttaacgt taaaccttac
     6601 ttgtgttgcc gcagtcattg caccaatagt tggtgttgaa atggttatgg tagaacctgc
     6661 aattcctgta at
//
LOCUS       DAOQNP010000109         6494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_4596_length_6494_cov_8.594502, whole genome shotgun sequence.
ACCESSION   DAOQNP010000109 DAOQNP010000000
VERSION     DAOQNP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4596_length_6494_cov_8.594502"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKY09_11585"
     CDS             complement(<1..689)
                     /locus_tag="PKY09_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNB13535.1"
                     /translation="MSNDLVIDSSPSEVRIALLEEKKLVELNSEKKDRSFAVGDIYLG
                     KVKKLMPSLNAAFVDIGYEKDAFLHYLDLGPQFQSLLKFVKDTQSGRQNNATLNNFEL
                     EKDIIKTGKITQVMSSGLWLPVQIAKEPISTKGPRITTEISIAGRYFVVSPYYNHVSV
                     SSKIKSPEERNRLKRLALSIKPKNFGIIVRTVAEGKKVAELDKDIQDVLMRWNLFYDT
                     LKVAQPGQKVLG"
     gene            complement(944..1759)
                     /locus_tag="PKY09_11590"
     CDS             complement(944..1759)
                     /locus_tag="PKY09_11590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13536.1"
                     /translation="MSSFSKSTLIGVSAVVLVGLLFLLPRHKKSDHAAKIPATEGFSI
                     DQFEQQAKLSLDTTQHHQIDEFSSLKEPERFDSVAAFWDRNNVPGLAAHYMELLAKGA
                     PTEKSYINAAYRYFDAWKQAQDSLSRDYFVGKAIASYSEVLKINPENLNAKTDLGICY
                     AEGTNNPMQGITMLKEVVEKDPNHENALLNLGFLSVKSNQFDKALERFNKVLQINPSR
                     IDMYVFIGQTYVQMGNNEEAIKSFETYKSLSNNTKAIAEIDSYIKELQTKAAK"
     gene            complement(1768..2076)
                     /locus_tag="PKY09_11595"
     CDS             complement(1768..2076)
                     /locus_tag="PKY09_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNB13537.1"
                     /translation="MTKAELVNEISIKTGIEKMVVQNVVESMMKVVKNSLVQQDNVYL
                     RGFGSFIVKKRAQKTGRIITKNTAIIIPEHFIPAFKPAKTFTDKVKNAYKKKESQPQL
                     "
     gene            2304..4061
                     /gene="aspS"
                     /locus_tag="PKY09_11600"
     CDS             2304..4061
                     /gene="aspS"
                     /locus_tag="PKY09_11600"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNB13538.1"
                     /translation="MLRTHTCGELNISHKGQQVSLSGWVQRSRDLGGMTFVDLRDRYG
                     LTQLVFDMDSNAELCQQARTLGREFVVQCHGVVAERSSKNNKLATGEIEIIVEKIVIL
                     NRSEIPPFTIEENTDGGDELRMKYRYLDLRRSNVKANFELRHRMASAVRNYLDQQQFI
                     EVETPVLIKSTPEGARDFVVPSRVHNGQFYALPQSPQTFKQLLMVAGFDRYYQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFIEQEDILAVFEGLVIHLFKEVKNITIPPLHRMSYAD
                     AMKYYGNDKPDMRFDMKIVAFKDESCQQFIIPGKFKVVDEAEYVGGIVCKGCAEYTRK
                     QVDELTDWVKRPQIGAGGLIYIRYNTDGSIKSSIDKFYSQEELKTILEYAHAMPGDLL
                     LILTGIENKTRKALSELRLEMGNRLGLRDKNNYSCLWVYDFPLLEWNEESERFHAMHH
                     PFTSPKTEDVSLLKTNPGKVRANAYDLVINGVEIGGGSIRIFDKKLQAQMFEVLGFTE
                     EKAQEQFGFLMNAFQYGAPPHGGIAFGFDRLCSLFGGSESIRDFIAFPKNNAARDIMI
                     DAPSAIDETQLKELGIRTL"
     gene            4306..>6494
                     /locus_tag="PKY09_11605"
     CDS             4306..>6494
                     /locus_tag="PKY09_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13539.1"
                     /translation="MKKTLRLLVFMLCLAVSNISIAQVTTMIGNANGGNFDLAGGMAG
                     NSWAATSNISPTVNDAWVISTAAGSTSGTQAAYVSSNPAAGVPPYNYTFTTVDSLILA
                     RTLPVNIPVAQSCITLSFQWKCMGENDASNNDLDNFKVYFIPKAGPITTNDIQSQYQI
                     GSVWYNGSNVWNAVTLNLDPKFSGSGVNYTLAFVFTSDGLVGASANPPVAIDDVLLTR
                     QAATALPACVTYTSPSNGATGLSPCNVALTWNHSLGCNGATSYQVWTSTAFGGPYTMQ
                     GTTNAGSYNLTGLAASTTYYWYIIPVNSFGANTAGCNLFRYSFTTGTNPVVSGVPPYY
                     EDFEGCVNWTMYNGGLANQWHIGNATSAGGTRGMYVSNNSGATNAYTVNASSITHAMS
                     NTIIDLTGGGACINLAFSYRCAGENGYDWLDVFAVPVSYTPTAGVGIPGGAVGGVLPN
                     LSNPVRIAGPINGQSSYTTFTASLNALSGRQFRLVFSWRNDGSVGTQPPAAIDNISIT
                     STGGPVNDAPCTATFIPPLSVAGLYLPGSNICGSNADEPSAPGCWTNTGGAGQVNTVW
                     FRFRAPSSGCVKIRTERGSLYDTQIALYTRAPLTGIVPCGSGNTLTYVSCNDNRAACG
                     SSQYFNSEISQSGLSPGYDYYIAVDGKNGQVGTFTLFIMDGGVGCTNQYPPTPGADCS
                     SPNIVCGSSISVPNPGYQGFGSLCDFTGGGGNCLLSGDRGGAWYQVN"
BASE COUNT         1908 a         1225 c         1269 g         2092 t
ORIGIN      
        1 cccaacactt tttgtcccgg ctgtgctact tttaacgtgt cataaaataa attccagcgc
       61 atcagcacat cctgaatatc cttatcaagt tctgcaactt tctttccttc cgcaactgtc
      121 ctgactatga taccaaaatt tttaggctta atgctaaggg caagtcgttt caaacggtta
      181 cgctcttcag gactttttat ttttgatgaa actgaaacgt gattgtaata aggtgaaact
      241 acaaagtatc gtcctgcaat ggaaatctct gtagtaattc ttggtccttt ggttgaaatt
      301 ggctctttcg caatctgaac aggaagccac aaaccggaac tcataacctg agttatttta
      361 ccggttttaa ttatatcttt ttcaagttca aaattattca aagtggcatt gttctgcctt
      421 ccactttgtg tgtcttttac aaattttaaa agtgactgaa actgtggacc taaatccaga
      481 taatgcaaaa aggcatcttt ctcatagcca atatcaacaa atgcagcgtt gagtgaaggc
      541 atgagctttt ttaccttgcc caggtaaatg tcgccaacgg caaaacttct gtccttcttt
      601 tcgctgttta attcaaccag ctttttttct tcaaggaggg caatacgtac ttccgatgga
      661 cttgagtcta taactaaatc attgctcaag acgcaaattt taattgatta gagggtagat
      721 aaccctaaaa aacagatttt aactgagata atatacccct gttagatctg caaacacttg
      781 attatttttt cttgtgtctg ttttttctca atctcttctt acgcttgtga gtagccattt
      841 tatggcgttt tcttttctta ccgcttggca tgttatttta atatttttta tttaaattat
      901 tgttatagtg ttttttaagg tgtgcaaaag taatatattc tttttattta gcagcctttg
      961 tttgcaactc ttttatataa gaatctattt cggcaatagc cttggtattg ttggaaagcg
     1021 atttataagt ctcaaaactc tttatagcct cttcattatt tcccatctga acataggttt
     1081 gacctataaa tacatacata tcaattcgtg atggattgat ttgcagcact ttattaaatc
     1141 gttccaaagc tttatcaaac tgattggact ttaccgacaa aaaccctaaa ttgagtaatg
     1201 cattttcatg attagggtct ttttcaacca cttccttcag catggttatg ccctgcattg
     1261 gattgtttgt accctcagca tagcatattc ccaaatcggt ttttgcattc agattttccg
     1321 gattaatctt taacacttcc gagtaacttg caattgcttt acctacaaaa tagtctctgc
     1381 ttaaagaatc ctgtgcttgt ttccatgcat caaaataacg ataagctgca ttaatgtatg
     1441 acttttctgt aggtgctccc tttgctaaca gctccatata atgtgcagcc aatccaggca
     1501 cattatttct atcccaaaaa gctgctactg agtcaaatcg ctcaggctct ttgagactgc
     1561 taaactcatc aatctgatga tgttgagtcg tatcaagtga aagctttgcc tgttgttcaa
     1621 attgatcaat actaaaaccc tctgttgccg gaattttggc agcgtggtca ctttttttat
     1681 gtcttggaag aagaaataat aaaccaacaa gaacaacagc agaaacacca atgagtgtac
     1741 ttttagaaaa tgaactcatc agtcagatta cagctgtggc tgcgactctt ttttcttata
     1801 agcgttcttt actttatcag taaaagtttt tgcaggctta aaggcaggaa taaaatgctc
     1861 tgggataata attgcagtat tcttggttat gatacgacca gttttctgcg ctcttttttt
     1921 aacaataaaa ctaccaaaac ctctcaaata cacattgtct tgctgtacta aagagttttt
     1981 cactactttc atcatggact caactacatt ctgaaccacc attttctcaa taccggtttt
     2041 aatcgaaatt tcgttaacca actctgcttt tgtcatttta tataaaattt aaagttaata
     2101 tcaatttgtt tttatgggag cgcaaaagta atctttattt ggtgaaaatg aaaatattat
     2161 gtcaattata agtcactaat cgcttaacta ttaaaagtat tggaatacct gcgttaaaaa
     2221 tgggtttaga aatattatac tgttccataa ttcagaaggc actaaattct ctaatttcgg
     2281 cactctttta atttcaggac aatatgctac gtacacatac ttgcggtgag ctaaatattt
     2341 ctcacaaagg ccaacaggtt tcactcagtg gttgggttca gcggtcgcgc gaccttggcg
     2401 gaatgacctt tgttgacctg cgcgacagat acggattaac acaattggta tttgacatgg
     2461 atagcaatgc tgaattatgt cagcaggcac gtaccctcgg acgtgaattt gtagttcaat
     2521 gccacggtgt tgttgctgaa agaagcagta aaaacaacaa acttgcaacc ggtgaaattg
     2581 aaattatagt cgaaaaaatt gtcatcctca accgctcaga aattcctcct tttaccatag
     2641 aagaaaacac agatggtggt gacgagctac gaatgaagta tcgttatctt gacctgcgca
     2701 gatcgaatgt aaaagctaat tttgaattgc gacatcgaat ggcttcggca gttcggaatt
     2761 atttagacca gcaacaattt attgaggtag aaacacctgt tcttattaaa tctacccctg
     2821 aaggcgcacg cgattttgtt gtaccttcaa gagtgcataa cggtcagttt tatgctttgc
     2881 cgcaatcacc gcaaacattt aaacaactac tgatggtggc aggatttgac cgttattatc
     2941 agatagtaaa atgctttcgt gatgaagacc tccgagctga cagacaacct gagtttacac
     3001 agatagattg tgagatgagt tttattgaac aggaagatat tttagctgtg tttgaaggat
     3061 tggtcatcca tcttttcaaa gaggtaaaaa atattacaat acctccactg catagaatga
     3121 gttatgccga tgccatgaaa tattatggta atgataaacc ggatatgcgt tttgacatga
     3181 aaattgttgc ttttaaagat gaaagctgtc agcaatttat catacccgga aaatttaaag
     3241 ttgttgatga agcagaatat gttggaggaa ttgtttgtaa aggttgtgca gaatatacac
     3301 gtaaacaggt tgatgaattg acagattggg ttaagcgtcc gcaaattggt gcaggaggat
     3361 taatttacat acgttataac accgatggaa gtattaagtc gagtattgat aaattctatt
     3421 ctcaggaaga gctaaaaact attcttgaat atgcacatgc catgccagga gacttacttc
     3481 tgattcttac cggcattgaa aataaaaccc gaaaagcatt gagtgaactt cgtctggaaa
     3541 tgggaaaccg gttaggactc agagataaaa acaattattc atgcttgtgg gtatatgatt
     3601 ttccattgct tgaatggaat gaagagtcag agcgatttca tgccatgcac cacccattta
     3661 catcaccaaa aactgaggat gtttctttac tgaaaaccaa tccgggaaaa gtacgtgcca
     3721 acgcttatga tttggttatc aatggtgttg aaataggtgg tggatctatc cgaatttttg
     3781 ataaaaaact gcaagctcaa atgtttgaag tacttggctt tacagaggaa aaagctcagg
     3841 agcagttcgg ttttctgatg aatgcttttc aatacggtgc gccaccgcat ggtggaattg
     3901 catttggttt cgacagactt tgttctctgt ttggtggatc tgaatcaata cgtgatttta
     3961 ttgcttttcc aaaaaataat gctgctcgtg acattatgat tgatgcacct tctgccattg
     4021 acgaaacgca acttaaggaa ttgggcataa gaacacttta gctcattacc gacaaaacat
     4081 agcctttcac cctgaaattg gaattgttga gcgatttttt tcgtttttcc gttctatggt
     4141 gtattaaata gggggattct ttgtcacatt tctataaata atgcgtataa tttaatttat
     4201 ttttaaaaaa aacatacatt tttaaaatat gcatagtaaa tttggcaatt attaagttta
     4261 aatataaata aatatattag aagaacatct acaaaataat ttaacatgaa aaagactctt
     4321 cgtttattgg ttttcatgtt gtgtcttgca gttagtaata tttcaattgc tcaagttaca
     4381 acaatgatag gtaatgctaa cggtggcaac tttgatttag ccggtggtat ggctggaaat
     4441 agctgggcag ctacttccaa cattagtcct actgttaatg atgcatgggt tattagtaca
     4501 gctgccgggt ccacatcagg aacacaggcc gcctacgtat cgtctaaccc tgcagcaggc
     4561 gtgccgcctt ataactacac gtttactacg gttgactctt tgattttagc aaggacttta
     4621 ccggtaaata ttcctgtcgc tcaatcttgc ataaccttaa gctttcaatg gaaatgtatg
     4681 ggagaaaatg atgcaagtaa taacgactta gataacttca aggtatattt tattccaaaa
     4741 gcaggaccta ttacaacaaa cgacattcag tcgcaatatc agattggctc tgtttggtac
     4801 aatggttcca atgtatggaa tgcagtaact ttaaatcttg atcctaagtt ttcgggcagt
     4861 ggtgtaaact atactcttgc atttgtcttc acctctgatg gcttagttgg agcaagtgcc
     4921 aatccaccgg tggctattga tgacgtatta ctgacaagac aggcagcaac tgccctgcca
     4981 gcttgtgtaa cttatacttc tccaagcaat ggtgctaccg gtttatcacc atgtaacgtt
     5041 gcattgacct ggaaccattc tttaggttgt aatggtgcta ccagttatca agtatggaca
     5101 tctacagcat ttggaggccc ctatacaatg caagggacta ctaatgcagg atcttacaat
     5161 ttaacaggat tagcagcaag tactacttat tattggtata tcattccggt aaactcattt
     5221 ggagccaaca ctgccggttg taacctgttt cgctattcat ttacaacagg tacaaacccc
     5281 gttgtgagtg gtgtgcctcc ctattatgaa gactttgaag gttgtgtaaa ctggaccatg
     5341 tacaacggag gattagccaa tcagtggcac attggtaacg ccacaagtgc gggtggtacc
     5401 agaggaatgt atgtttctaa taatagtggt gccaccaatg cttatacggt aaatgcatca
     5461 tcaattacac atgccatgag caacactatt attgacctta ctggtggtgg tgcttgtatc
     5521 aatttggcat tttcttaccg ctgtgctgga gaaaatggtt atgactggct ggatgtattt
     5581 gcagtaccgg tttcttatac tccaactgcc ggtgtaggaa taccgggtgg tgcagttgga
     5641 ggagtattac ccaatttatc taacccggtt cgaattgcag gacctataaa cggacaatca
     5701 agttatacta cttttacggc ctcactcaat gctttatcag gaaggcaatt ccggttggta
     5761 tttagctgga gaaacgatgg ttctgttgga acacagccac cagcagccat tgataatatc
     5821 agcataacat ctaccggtgg acctgtaaat gacgcacctt gtacagcaac atttattccg
     5881 ccattatctg ttgcaggatt atatcttccc ggtagtaata tatgtggaag taatgcagac
     5941 gaaccaagtg cacccggatg ttggactaac actggcggtg ctggccaagt caatacggtt
     6001 tggtttcgtt tccgggcacc cagtagtgga tgcgtaaaaa tccgcacaga aagaggctct
     6061 ttatatgata ctcaaattgc attatatacc agagcaccat taacaggaat tgtaccttgt
     6121 ggatctggaa atacattaac ttatgtaagt tgtaatgaca atcgtgcagc ttgtggttct
     6181 agtcaatatt tcaactctga aatatcccaa tccggcttat caccgggata tgattattac
     6241 atagccgtag atggaaaaaa tggtcaggta ggaactttta ccctctttat tatggatgga
     6301 ggtgtaggat gtaccaatca gtatccacct actccgggtg cagattgctc ttcaccaaat
     6361 attgtttgtg gatcttcaat ttctgttcct aacccaggtt atcagggatt tggatcactt
     6421 tgtgatttta caggtggcgg aggtaattgc ctgctttcag gtgatagagg tggtgcctgg
     6481 tatcaggtta atat
//
LOCUS       DAOQNP010000110         6432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_4660_length_6432_cov_7.971774, whole genome shotgun sequence.
ACCESSION   DAOQNP010000110 DAOQNP010000000
VERSION     DAOQNP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_6432_cov_7.971774"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="PKY09_11610"
     CDS             <1..2302
                     /locus_tag="PKY09_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNB13540.1"
                     /translation="QLWMSGAEINWQNFYALEKRKRIALPTYPFEKKRYWLDPIKSTT
                     NIKSEISYSDSVYLNKDSGFEINQQQLNTTTMNRKDLITAELKTILEESSGMELGNDS
                     SATFLDLGLDSLFLTQAALTISKKYGVKITFRQLNEEFSSLQSLSAHLDANLPADAMP
                     TAKVASQQVPQQTNIAPQQPAIQTSGMNPMQMMMMQQMQMMQMMMQQMNQPQQQVAPS
                     VQAHIEKAVEIKSHTKKEDVSENEKAELAKPFGAIARIEKASANDITAEQEKWLKQFI
                     TAYTEKTKASKKYCQDYRSVLADPRVVTGFKPALKEIIYQVVVNKSKGVKFQDIDNNE
                     YVDVLNGFGSNFFGWGNDMLKNVWKKQLDDGIELGPQTPLAGECAQLICELTGYDRAG
                     FCNTGSEAVLGAMRIARTVTGRNLIICFNGSYHGINDEVIVRGTKKLKSFPAAAGIMP
                     ESVQNMLVLDYGTEESLQIIRERMDEIAAVMIEPIQSRRADFHPKEFIHEVRKLTKEN
                     NVLMIFDEVITGFRLRLGGAQEYYGVDADLSTYGKVIGGNMPVGAIAGKKQYMDALDG
                     GFWQYGDNSIPEVGVTYFAGTFVRHPLAMAAMKEVLLYLKKEGKPLYEKLNAKTQRLV
                     DEVNAHSKKLGSPFKLVTFGSLFKGKWETEPHYTELLFAMMRYKGVHIMDGFPCFLTL
                     AFTDADVDFVISKFKESLSELCEIGFIPSDKNNDHSQITSNGHLQNGNGQHPPIPGAR
                     LGKDANGKDAWFIADPERPGKFLMVN"
     gene            complement(2383..2454)
                     /locus_tag="PKY09_11615"
     tRNA            complement(2383..2454)
                     /locus_tag="PKY09_11615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2419..2421),aa:Arg,seq:ccg)
     gene            complement(2485..5850)
                     /gene="mfd"
                     /locus_tag="PKY09_11620"
     CDS             complement(2485..5850)
                     /gene="mfd"
                     /locus_tag="PKY09_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008507685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNB13541.1"
                     /translation="MDLLKDLKEKYIAHPAVKELFLQISKQASKTHLQNTAGSASSFI
                     ASATFENEPAPMLFILPDREEAAYFLNDLENLNPGHSVFFFPSSYRKPFKLNAHENAS
                     IQMRTEVLSHINKNSKFFIVVSYAEAVAENVVTRHALEKNTIEARVGEQLSIDFVNDF
                     LTENNFQRTDFVFEPGQFAIRGGIVDVYSYAYELPFRLEFDGNKIESIRSFNPVTQLS
                     EKNLHFVTIIPNIQTEMKQNVTESFFKFLPENTLIWTRDLSYSLNYLDDNLTVLKNQS
                     PENDEDEKGDDPKIVYDSIEDIKSVLSTFKQVEFGLKSFYENAVGIKFNVQPQPQFNK
                     NFNLLAGHLKANSDKHFKSYIFSDSAKQVERIYAIFDDLSFKKEVQRDDIQFSPVYTS
                     LYKGFTDLDLQVSVFTDHQIFDRYHRFKLRKNYSRNEAISIKELYSLKPGDYVTHIDH
                     GVGRFGGLEKLNIEGRQQEAIRLVYKDNDILYVSIHSLHRIARYTGKEGTAPSLNKLG
                     STSWASLKQKTKKKVKDIARDLIALYAKRKAQEGFAYAPDNYLQTELEASFIYEDTPD
                     QIKATRDVKKDMESVSPMDRLICGDVGFGKTEIAIRAAFKAVNDSKQVAILVPTTILA
                     MQHYRTISERLKDFPCSIDYINRFKSTAQQNETLRKLAAGKIDIIIGTHRLLSKDIKF
                     SNLGLLIIDEEQKFGVASKEKIKSLKVNVDTLTLTATPIPRTLQFSLMGARDLSIINT
                     PPPNRYPVTTELHTFNDKVIREAIMYEVSRGGQVFFVHNRVQDIHDISDMIRKLCPDV
                     KIVVGHGQMDGSQLEEVLMTFIEGDADVLVATTIIESGIDISNANTIIINQAQNFGLS
                     DLHQMRGRVGRSNKKAFCYLLAPPLTVLTPEARQRLQAIEEYSDLGMGFQVALRDLDI
                     RGAGNLLGGEQSGFISEIGFEMYQKILDEALIELRESDFKELFPADANKPIVADCQIE
                     TDLEILIPTQYVENIVERLNLYKELDDVETEEKLVAFENQLRDRFGPVPKQVMELFET
                     IKLRWKAKSLGFEKLILKNGQLKGYLVSNPDSRLYKSDLFMQIINFVKSNPSRCRMKE
                     DKSKLSLTFYKIETVNEANAVFRQMMKPSVATA"
     gene            5884..6024
                     /locus_tag="PKY09_11625"
     CDS             5884..6024
                     /locus_tag="PKY09_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13542.1"
                     /translation="MSLRNGGYLEQKFNRRTALEPTTRLAFEALYPPLHQTAVGAVFR
                     VD"
     gene            complement(6170..>6432)
                     /locus_tag="PKY09_11630"
     CDS             complement(6170..>6432)
                     /locus_tag="PKY09_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010423669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13543.1"
                     /translation="YDSSDISVEFRKDIYNEKYLQSLDLNERQVKALLFTKDKGKITN
                     RQYQTLNNCSRNTASNDLSELVDKEFLKPSGQKGAGAFYTLK"
BASE COUNT         1986 a         1232 c         1212 g         2002 t
ORIGIN      
        1 tcaactgtgg atgagtggtg cagaaattaa ctggcaaaat ttttatgccc ttgaaaaaag
       61 aaaacgcatt gcattaccca cctatccatt tgaaaaaaag cgatactggc ttgacccaat
      121 aaaatctact acaaatatta aatctgaaat atcatactca gattctgttt atcttaataa
      181 ggattcagga tttgaaatca atcaacaaca attgaataca acaacaatga atagaaaaga
      241 tcttatcaca gcagagctaa aaacaattct tgaagaatcg tcaggtatgg aacttggcaa
      301 tgacagtagt gcaacctttc ttgatttagg tttggattca ctttttctga cacaggccgc
      361 cctgaccatc agtaaaaaat atggagtaaa aattactttc agacaactta atgaagagtt
      421 ttcctcgctg caatctctgt cagcacattt agatgccaac cttcctgcag atgctatgcc
      481 tacagcaaaa gttgcatcac aacaagtgcc acaacaaaca aatattgccc cgcaacaacc
      541 tgcaatacaa acatcaggca tgaaccccat gcagatgatg atgatgcagc aaatgcagat
      601 gatgcagatg atgatgcaac aaatgaatca gccgcaacaa caagttgcac catctgttca
      661 ggcacatatt gagaaagctg ttgaaataaa atcgcatact aaaaaagaag atgtcagcga
      721 gaacgaaaaa gctgaattgg caaaaccatt tggcgctatt gccagaattg aaaaagcatc
      781 ggctaatgac attactgctg aacaagaaaa atggttgaaa caatttatta cagcatacac
      841 tgaaaaaaca aaagcctcaa aaaaatattg tcaggactat cgcagtgtgc ttgccgaccc
      901 acgtgtggtt acaggattta agcctgcctt aaaagaaatt atctatcagg ttgtcgttaa
      961 caagtctaaa ggtgtaaaat ttcaggacat tgacaacaat gaatatgtag atgtattgaa
     1021 tggttttggc tccaactttt tcggatgggg aaatgacatg ctgaaaaatg tatggaaaaa
     1081 acaattggat gatggtattg aacttggtcc gcagacacca cttgccggag agtgtgcaca
     1141 attaatttgt gaactcacag gatatgaccg tgccggtttc tgcaatacag gtagtgaggc
     1201 tgtactcggt gccatgagaa ttgcaagaac ggttacaggt agaaatctaa ttatttgttt
     1261 taatggctca tatcatggta ttaacgatga agtgattgtt cgtggaacaa aaaaacttaa
     1321 atcgtttcct gccgctgcgg gaattatgcc ggagtcggtg caaaacatgc ttgtattaga
     1381 ttacggtaca gaagaaagtt tacagataat ccgtgaacgt atggatgaaa ttgctgccgt
     1441 aatgattgaa cctattcaat cacgcagagc tgattttcat ccaaaagaat ttattcatga
     1501 ggttagaaaa ctaaccaaag aaaacaatgt gttgatgatt tttgatgagg tgataacagg
     1561 attcagatta agacttggtg gtgcgcagga gtattatggt gttgatgccg acctgagtac
     1621 ttatggaaaa gtaattggcg gcaatatgcc tgttggagct attgcaggta aaaaacaata
     1681 catggatgct ttagatggtg gattctggca gtatggcgac aactctattc ctgaagttgg
     1741 tgtaacctat tttgcaggaa catttgtacg tcatccgctt gctatggcag caatgaaaga
     1801 agttttgttg tatctaaaaa aagaaggtaa gccgctatac gaaaagctga atgctaaaac
     1861 acaacgatta gttgatgaag ttaatgcaca tagtaaaaaa ttaggctcac catttaagtt
     1921 agtaaccttc ggctcactat ttaaaggtaa atgggaaaca gaaccacatt atacagagtt
     1981 actctttgca atgatgcgct ataaaggggt tcacatcatg gatggttttc catgcttctt
     2041 aacacttgcc tttactgatg cagatgttga ttttgtaatt tctaaattca aagaaagctt
     2101 atcagaactt tgcgaaatag gtttcattcc ttcggataaa aacaacgatc attctcaaat
     2161 aacttctaac ggacatcttc aaaatggcaa tggacaacat cctccaatac ccggagcaag
     2221 attaggaaaa gatgccaatg gaaaagatgc ctggtttatt gccgaccccg aaagaccggg
     2281 taagttttta atggtgaatt aatattttgc tttgagtcgt ttaacaataa taaacaaagt
     2341 gtttgctgtt ggtgcttctt tagaatccgt ttggaattaa agtggtcctg tcgggaatcg
     2401 aactcgaatc tcaggttccg gagacctgcg tactatccgt tatactacag gaccaaatgc
     2461 ggctgcaaat gtacaataat caatctatgc tgttgccaca gagggtttca tcatttgccg
     2521 gaaaacagca ttagcctcat taactgtttc tatcttataa aaggtaagac taagctttga
     2581 cttatcttct ttcattcggc agcgtgatgg attgctttta acgaaattga taatctgcat
     2641 aaacaaatca cttttataca atcgcgaatc aggattggaa accaagtatc cttttaattg
     2701 tccattcttt aaaatcagtt tttcaaaacc taatgacttt gctttccatc ttagtttgat
     2761 ggtttcaaac aactccatta cctgctttgg tacagggccg aatctgtcgc gaagctgatt
     2821 ttcaaatgca actaattttt cttctgtctc aacatcatct aactctttgt aaaggtttaa
     2881 tcgctcaaca atattttcaa catactgtgt aggaatcaat atttcaaggt ctgtttcaat
     2941 ctggcaatcg gcaacaattg gtttgttggc gtcagcagga aataattctt taaaatcaga
     3001 ctcgcgcaat tctatcaaag cttcatccaa aattttctga tacatttcaa atccaatttc
     3061 agaaatgaaa ccactctgtt cgccacccag caaatttcct gcaccacgaa tatctaagtc
     3121 gcgtagtgca acctggaatc ccattcctaa atcagagtat tcttcaattg cttgcagccg
     3181 ctgacgtgcc tcaggtgtta acactgtgag tggtggtgca agcagataac aaaaagcttt
     3241 tttattgctt cgccctaccc tgccacgcat ctgatgcaaa tcgctcaatc cgaaattttg
     3301 tgcctggttg atgataatgg tgttggcatt gctgatatca attccggatt cgatgatggt
     3361 tgtggccacc aaaacatctg catcaccttc aataaaagtc ataagcactt cttcaagttg
     3421 gctgccgtcc atctgtccgt gaccgacaac aatttttaca tcagggcaaa gcttcctgat
     3481 catatcagaa atatcgtgaa tatcctgaac acgattgtgt acaaaaaaca cctgacctcc
     3541 acgtgatact tcatacatta ttgcttcgcg aataactttg tcattaaaag tatgtaactc
     3601 tgttgtcaca ggataacggt ttggtggtgg tgtgttgatg attgataaat cacgcgcacc
     3661 cattaatgaa aactgaagcg ttcttggaat aggagttgct gtcaatgtca gtgtgtcaac
     3721 atttacttta agcgatttga ttttctcttt acttgcaacg ccaaatttct gctcttcgtc
     3781 aataatcaac aatccaagat tactgaattt aatatcctta ctcagcaatc tgtgcgtgcc
     3841 aatgataatg tcaatcttac ctgctgcgag tttacgcagt gtttcatttt gctgtgcagt
     3901 tgacttaaaa cgattgatat aatcaatact acaagggaaa tctttcaacc tttcgctaat
     3961 agttctgtaa tgttgcattg ccaatatggt agtaggaact aaaatggcaa cctgcttact
     4021 gtcgtttaca gctttaaaag cagcacgaat agcaatttca gtttttccaa atccaacatc
     4081 accacagatt agcctgtcca ttggcgatac gctttccata tctttcttta catcacgtgt
     4141 tgcctttatc tgatcgggag tgtcttcata aataaaactt gcctccaatt ctgtttgcag
     4201 ataattatca ggagcatagg caaaaccttc ctgtgctttt cgtttggcat ataatgcaat
     4261 cagatctcgg gcaatatctt ttaccttctt ctttgttttt tgtttgagtg atgcccacga
     4321 tgtgctaccc aacttgttga gtgatggcgc agttccttcc ttacctgtat aacgtgctat
     4381 gcgatgcaga ctgtgaatac ttacataaag aatatcgtta tctttatata ccagtctgat
     4441 tgcttcttgt tgacgtccct caatgtttag tttctccaat ccaccaaaac gtccgacacc
     4501 atgatcgata tgcgttacat aatcaccggg cttaagacta tatagttctt ttatggaaat
     4561 agcctcattg cggctgtaat ttttacggag tttaaaacga tgataacgat caaatatctg
     4621 atggtctgta aagacagaaa cttgaagatc taaatcggta aagcccttgt acaatgaagt
     4681 gtaaacagga ctaaactgta tatcgtcacg ctgaacttct ttcttaaaac ttaaatcatc
     4741 aaaaatggca tagatgcgtt cgacctgttt ggcagaatct gaaaaaatgt atgacttaaa
     4801 atgcttatca ctattcgctt ttaaatgacc tgccagcaaa ttaaagtttt tattgaactg
     4861 tggttgtggc tgtacattga attttattcc aactgcattt tcataaaatg attttaaacc
     4921 aaactcaacc tgtttgaaag ttgacagaac agatttaata tcctctatgg aatcataaac
     4981 aattttggga tcatcgccct tttcatcttc atcgttttca ggagattgat tttttagtac
     5041 tgttaaatta tcatcaaggt agttgagtga ataagataga tcgcgtgtcc agattaaagt
     5101 attttcagga agaaatttaa aaaacgactc tgttacattc tgcttcattt ctgtttgaat
     5161 gtttggaatg atggtaacaa agtgcagatt tttttcagac aattgcgtca ccggattgaa
     5221 tgagcgaatg gattcaattt tattaccatc aaactccaat ctgaaaggca actcataggc
     5281 ataagaataa acatcaacaa tgcctcctct tatggcaaac tgtcccggtt caaaaacaaa
     5341 atctgttctt tgaaaattat tttctgtcag aaaatcattt acaaaatcaa tgctgagttg
     5401 ctctcctaca cgtgcttcaa ttgtattttt ctcgagtgca tgtcgtgtta caacattctc
     5461 ggcaactgct tcggcatacg atacaacgat aaaaaactta ctgtttttat tgatatgact
     5521 aagcacttct gtacgcatct gaattgatgc attttcatgt gcgttgagtt taaagggttt
     5581 tctgtaggat gaagggaaga aaaaaacact atgcccgggg ttcaggtttt ctaaatcgtt
     5641 aagaaagtaa gcagcttctt ctctatcagg taaaataaaa agcattggtg caggctcatt
     5701 ctcaaaagtg gcagatgcaa tgaaagacga agcagaaccg gcagtattct gaagatgtgt
     5761 ttttgatgct tgcttactta tttgcaaaaa gagttccttt acagcagggt gtgctatata
     5821 tttttctttt aaatctttaa gtaaatccat tcttaaaaaa acggaatgca aaactaacgt
     5881 tttatgtctt tgcgtaatgg cggatattta gaacaaaagt tcaatcgaag aactgcactt
     5941 gaacctacca caagactggc atttgaagca ctgtacccac cattacacca aaccgctgtt
     6001 ggtgctgtct ttcgtgttga ttgaccgtct tttgagcgtg ggaacaggca cactctttgc
     6061 caatttttgg cttgcgtgtc ggctactgcg acttggcaat gtttgtgact gtccgtcatt
     6121 gtgcaatttg tgcaattaat gtgcaattga ttaatgtctg ttcctcctct catttcaatg
     6181 tgtaaaatgc tcctgctccc ttttgtccac ttggttttaa gaattcttta tcaaccaact
     6241 ctgacaggtc gttactggct gtatttctgg aacagttgtt taatgtttga tactgtctat
     6301 ttgtaatttt ccctttgtct tttgtaaaca aaagagcttt tacttgtcgc tcgttcaggt
     6361 caagtgattg cagatacttt tcattgtaaa tatcttttct aaactccaca gaaatgtcgc
     6421 ttgaatcata ac
//
LOCUS       DAOQNP010000111         5647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_5711_length_5647_cov_6.717990, whole genome shotgun sequence.
ACCESSION   DAOQNP010000111 DAOQNP010000000
VERSION     DAOQNP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5711_length_5647_cov_6.717990"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /gene="miaE"
                     /locus_tag="PKY09_11635"
     CDS             complement(<1..223)
                     /gene="miaE"
                     /locus_tag="PKY09_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HNB13544.1"
                     /translation="MFKLKLPTDPRWVNIVETNIPEILTDHAWCEQKAASNAISLIVR
                     FPEYTDMVKVLCDIAREEMEHFRMVVEKME"
     gene            complement(230..1738)
                     /locus_tag="PKY09_11640"
     CDS             complement(230..1738)
                     /locus_tag="PKY09_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNB13545.1"
                     /translation="MIRYFFTLIFAFVILFAKAQKSPSKIELIHANTLEGDDALGKDV
                     RRLLGDVVFKQENTFMYCDSAYLYSATNSMDAFGNVRIEQGDTISLTGDILKYNGNSR
                     QARLLNNIVFSDRKMTLISQNLNYNLDTGIADYIEGGQIVDGTDTLTSRFGTYFSNQK
                     KLAFKKDVVLKHPGHRMETDTLVYGTVSKVAEFYGPCYIRSNDSSVIYCENGWYNTAT
                     DKAFFSKNAFVQNKNQILKGDSLFFDNNTSAGEAFKNVSITDTTEKVVVNSDYGNYNG
                     EKGYSFMTGNLLMTKMFEKDSLFMHGDSLISLFDSTGKAISYAVYHNVRMYKKDLQAT
                     CDSLIYTAADSLLFLYHNPVLWNEVNQLTADTISMLISSEGIRKLFLNQAAFITSKED
                     SIRFNQLRGKTMVGYFEDNMLSKIDVFGNGQSIYFARNKKNELTGVNRADCSNMIIII
                     KDSKISKLKLIEKPDATFYPIGELSASELMLKGFSWQENKRPVDKNDIFRKN"
     gene            2023..2358
                     /locus_tag="PKY09_11645"
     CDS             2023..2358
                     /locus_tag="PKY09_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13546.1"
                     /translation="MDQQFEKIKAIESKIQKLIELNKNLAEEKAMYEEENRQLTEQVK
                     NLKEKMHQYEQKIDMQGQQLTQLEETNKSLNLATNETTETKEENKFLKSRIDEYVQEI
                     DKCLALLDK"
     gene            2366..2677
                     /locus_tag="PKY09_11650"
     CDS             2366..2677
                     /locus_tag="PKY09_11650"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNB13547.1"
                     /translation="METVSMLINVGNRQLNLKVPAADQENILKAEKLLNDKITALRKS
                     YAVNDNQDILAMCAFSIAGELISQQASGKKSENTRMEKLESLDALLNGFLAQPELFNK
                     Y"
     gene            2841..4400
                     /gene="rny"
                     /locus_tag="PKY09_11655"
     CDS             2841..4400
                     /gene="rny"
                     /locus_tag="PKY09_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNB13548.1"
                     /translation="MDPIIIGVGIGAALIAAIITFLVTKKNNAVIVGAAKQQADTLLK
                     EAEAKGEVIKKDKLLEAKEKFFQLKSEHEKAIAEKDKKISDAENRIRQKERELNQKLE
                     QNSRKHQEYEALQTNLKSQMQIVEARKQEVDKSLQKHVQALEKIAGISQDEAKTQLME
                     TLKAEARTQAQSYIKEIMDEAKLTANKEAKRIIIQSIQRVATEHAVENSVTVFNLEND
                     DVKGRVIGREGRNIRALEAATGIEIIVDDTPEAIILSGFDPVRRELARLALHQLVSDG
                     RIHPARIEEVVNKVRKQLEEEIIETGKRTCIDLGIHGLAPELIRMVGRMKYRSSYGQN
                     LLQHSREVANLCAIMASELGLNAKLAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKN
                     KENAEVCNAIGAHHDEIEMTSLISPIIQVCDAISGARPGARREIVEQYIKRLKDLEAL
                     ALSYPGVEKSFAIQAGRELRVIVESEKVSDQQADTLSFDLAQRIQTEMTYPGQVKVTV
                     IREKRAVNYAK"
     gene            4420..>5647
                     /locus_tag="PKY09_11660"
     CDS             4420..>5647
                     /locus_tag="PKY09_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNB13549.1"
                     /translation="MAESEHKEYYRNLITLVTGSAFAQLISLALSPVFSRIYTPHEFG
                     LLAVFMSILSALSVIVCLRYEFAIIPARTGKDAFHLLELCFYSTLLTTILTLIGVTIY
                     NLFISTNTVLSKWFWFLPFMVLINGSYQAMLSWANRHKSYKVISRYRITNSVITNIII
                     LGAGLAGFISMGLLAGFVSGGAVAAIMLYFNIQKQSEPFKLGFNLAALKNVAKKYEAF
                     PKVNLWQSIADMLLINGVIYFLTAFFSPAVVGLYGLTMRVLQAPFNLIGYSMGQVFIQ
                     DAAHQYNEGKNIQPIIKKSIQRSAFIAIPIVLPLILAGPQLFAFVFGEQWRQSGEYAQ
                     ILAPWFFLDFIRTPLGQIPIIMGKQKRQFTITIAGNILMVISLLIGGFYFNKPLLMVT
                     LFTALQSVYLIYVIYWI"
BASE COUNT         1839 a         1076 c         1054 g         1678 t
ORIGIN      
        1 gttccatttt ctcaaccacc attctgaaat gctccatttc ttctctggca atatcgcata
       61 acactttcac catgtctgta tattccggaa aacgaactat cagtgatatg gcattacttg
      121 cagccttctg ctcgcaccat gcatgatcag taagaatttc aggaatgttt gtctcaacta
      181 tatttaccca tcgtgggtct gtcggcaatt ttagtttgaa cattatacat taatttttcc
      241 gaaagatatc attcttatcc acaggccgtt tattttcctg ccagctaaaa ccctttaaca
      301 tcaactccga agccgaaagt tcgccaatcg gataaaatgt agcatcaggt ttttcaataa
      361 gtttaagttt acttatttta ctgtccttaa ttattataat catgttgctg cagtcggcac
      421 gattgacacc tgtaagctca ttctttttat ttcttgcaaa ataaatactc tgaccattgc
      481 cgaaaacatc tattttactc aacatattgt cttcaaaata tcctaccata gttttccctc
      541 taagctgatt aaatcgaatg ctatcttctt tagaggtgat aaatgcggcc tgattaagaa
      601 ataatttccg gataccttca gatgagatga gcatagagat agtatctgca gttagctgat
      661 tgacttcatt ccacaacaca ggattatgat ataaaaacaa caaactgtcg gcagcagtgt
      721 aaatcaacga atcgcaggtt gcctgcaaat cttttttata cattcttaca ttgtgataaa
      781 cagcataaga aatagcttta cctgttgaat caaacaaaga aataagactg tcgccatgca
      841 taaacaaact gtctttttca aacatctttg tcatcagcag gtttcctgtc ataaaagaat
      901 agcctttctc cccattataa tttccatagt cggaattgac aacaaccttt tctgttgtat
      961 cggtaatact tacattttta aatgcttctc ctgccgaggt attattatca aaaaacaagc
     1021 tgtcgccttt aagaatctgg tttttattct gcacaaaagc atttttactg aagaaagctt
     1081 tatcggtagc tgtgttgtac catccgtttt cacaatatat aacagaactg tcgttggaac
     1141 gaatataaca agggccataa aactcagcaa ctttactaac ggttccataa accaaggtgt
     1201 cggtttccat tctgtgtccg ggatgtttga gaacaacatc cttcttgaag gctaatttct
     1261 tctgattact aaagtatgta ccaaagcggc tggttaaagt atctgtgcca tctacaatct
     1321 gcccaccttc aatataatct gcaatacctg tatcgagatt ataatttaga ttttgcgaaa
     1381 taagcgtcat ttttctgtcg ctaaaaacaa tattgttgag taggcgcgcc tgcctgctgt
     1441 tgccattata ctttagtatg tcaccggtga ggctaattgt atctccctgt tcaatccgca
     1501 cattcccaaa agcatccata ctgtttgttg cagaataaag ataggcgctg tcacaataca
     1561 taaaagtgtt ttcctgttta aaaaccacat cgcctaaaag ccgtcttaca tcttttccta
     1621 aagcatcatc accctctaat gtgttggcat gtatcaattc tatttttgaa ggcgattttt
     1681 gagccttggc aaatagtatt acaaaagcaa aaatgagtgt gaaaaaatac ctgatcatga
     1741 agttggcaaa aataagaggt ttcagcacat ctctgtaaca gcagtgttta ttacaacaac
     1801 ttggtttaat caatataaac aactgtttta caatcaaata atcaatgtgg atttaataag
     1861 attttaactt gttattaccg acctcattat tgtatttaag ggaaagttgc cctacttctg
     1921 actaattaac aaagtattgt tgctaatttt tagtttaaca gggttgtaat acagtcatga
     1981 ataatatttt tactgaatca aaaaccattt aatcaaataa atatggatca gcaattcgaa
     2041 aaaatcaaag caattgaatc aaaaattcaa aaacttattg aactcaataa aaacctggcc
     2101 gaagaaaaag ccatgtatga agaagaaaac cgtcagttga cagagcaggt taaaaacctt
     2161 aaagaaaaga tgcatcagta tgagcaaaag attgatatgc aggggcaaca gttgactcag
     2221 ttggaggaaa caaataaatc acttaattta gccaccaacg aaacaacaga aacaaaagaa
     2281 gagaataaat ttttgaaatc aagaattgac gaatacgtgc aggagattga caagtgtctt
     2341 gcattattgg ataagtaaga ataaaatgga aacagtatcc atgcttataa atgtcggtaa
     2401 cagacaactg aacctaaagg ttcctgctgc cgatcaagag aatatcttaa aagctgaaaa
     2461 acttttaaat gataagatta ctgcattacg caagtcgtat gctgtcaatg acaatcagga
     2521 tattctggca atgtgtgcat ttagtattgc tggcgaactt ataagccaac aggcttccgg
     2581 caagaaatca gaaaataccc ggatggaaaa actggagtcg ctggatgcat tattaaacgg
     2641 ttttctggcg cagccggagt tgtttaataa atactaacaa accatattca tggctacaca
     2701 cgctgtgcag cctgttcatt ctgctttttc taaaacgtta aactgatttc aaatctttag
     2761 acttaagatt ggtttcgtgt tttgcggaat gaaagtttaa tcacatctcg tttagggata
     2821 taaaattatt ttattaaaaa atggatccaa taattatagg agttggaata ggtgcagctt
     2881 taatagcagc aattattacc ttccttgtta caaaaaagaa caatgctgta attgtaggtg
     2941 cagcaaaaca acaggctgat acactgctta aggaagctga agctaaaggc gaggttatta
     3001 aaaaagacaa actgcttgaa gcaaaggaga aatttttcca gttaaaatca gagcatgaga
     3061 aggcgattgc cgaaaaagac aagaaaattt ctgacgcaga aaaccgtatc aggcaaaaag
     3121 agcgtgaact gaaccagaaa cttgagcaaa attcgcgcaa gcatcaggaa tatgaagctt
     3181 tgcagaccaa cttaaaaagt cagatgcaga ttgtagaagc tcgcaagcaa gaggttgaca
     3241 aatcgcttca aaaacatgtt caggcactag aaaaaattgc aggcatcagt caggatgaag
     3301 ctaaaacgca gttaatggaa acgcttaaag ccgaagcacg cactcaggcc caatcctata
     3361 tcaaagagat tatggatgaa gcaaaactaa ctgcaaacaa agaagcaaaa cgcatcatca
     3421 ttcaaagtat tcaacgtgtg gcaacagaac atgccgttga aaattcagtt acagttttca
     3481 atcttgagaa cgatgacgta aaaggccgtg taattggccg cgaaggacga aacatcagag
     3541 cacttgaagc tgcaacagga atagaaatta ttgttgatga cacacctgaa gcaattattc
     3601 tgagtggctt tgatcctgta agacgtgagt tagcacgtct ggcacttcat cagttagtga
     3661 gtgacggacg tattcatcct gcccgtattg aagaggtagt gaataaagta cgtaagcaac
     3721 ttgaagaaga aattatagaa accggtaaac gcacttgtat tgacttagga attcacggac
     3781 ttgcacctga attaattcgt atggtaggta gaatgaaata ccgatcttct tatggtcaga
     3841 atttgttgca acactcgcgc gaggtagcca acctttgtgc aataatggcc agtgaattag
     3901 gattaaatgc aaagcttgct aaacgtgccg gtctgctgca cgatattgga aaagtgcccg
     3961 atgatgaacc tgaattacca catgctattc tgggtatgaa acttgccgaa aagaataaag
     4021 aaaatgctga agtatgtaat gccataggtg cccaccacga tgaaattgaa atgacaagcc
     4081 tcatcagccc tatcattcag gtttgtgatg ccatttcagg tgcacgcccg ggtgccagac
     4141 gcgaaatcgt tgagcaatac attaagaggt taaaagacct tgaagcactt gccctctcct
     4201 atcctggtgt tgaaaaatct tttgctatcc aggcaggacg cgaattgcga gttattgttg
     4261 aaagtgaaaa agttagtgac cagcaagccg atacactcag ctttgacctt gcacaaagaa
     4321 ttcagacaga gatgacctat cccggacaag taaaagttac ggttatccgt gagaaacgtg
     4381 cggtaaacta cgccaagtaa cagccacaag gcaggtttca tggcagaatc ggaacataaa
     4441 gagtattatc gcaacctgat tactttagtt accggcagcg catttgcgca gttgattagc
     4501 ttggcattgt cccccgtatt ttccagaata tacacaccac atgagtttgg cttgttagct
     4561 gttttcatga gtatattaag tgcattgtct gtaatagttt gtttgcgtta tgagtttgcc
     4621 atcatacctg cacgcaccgg aaaagatgca tttcaccttc ttgaactttg tttttactct
     4681 accttgctga ctactatatt gacgctgata ggggtaacca tctacaatct gtttatttca
     4741 accaatactg tcttgtcaaa atggttttgg tttctgcctt ttatggttct gattaacggc
     4801 tcctaccagg caatgcttag ttgggcaaac agacataaaa gctacaaagt tatttcacga
     4861 tacagaataa ctaattcagt tattaccaat attattattc taggtgcagg acttgctggc
     4921 tttatttcta tgggacttct tgcaggtttt gtttcaggtg gtgctgttgc cgccatcatg
     4981 ctatatttta atattcaaaa gcagtctgaa ccttttaagc ttggctttaa tttggctgca
     5041 ttaaaaaatg ttgcaaaaaa atacgaagct tttcctaaag taaatctatg gcagtcaatt
     5101 gccgatatgc tgttaattaa tggagtcatc tattttctga ctgcattttt cagccctgca
     5161 gtagtaggtc tatacggact taccatgaga gttttacaag ctccttttaa cttgatagga
     5221 tacagcatgg gccaggtttt cattcaggat gcagcacatc agtataatga aggaaaaaac
     5281 attcaaccca ttattaaaaa atctattcaa cgttctgcat tcattgcaat acctattgtg
     5341 ctaccattaa ttttagcagg accacaactt tttgcatttg tttttggcga acagtggcgc
     5401 cagtcgggcg aatatgcaca aatacttgca ccttggtttt tccttgactt tatcagaaca
     5461 ccattaggac agattccgat catcatggga aagcaaaaac gacaattcac cataacaatt
     5521 gccggcaata ttctaatggt aatttctttg ttgataggtg gcttttattt taataaaccg
     5581 ttactcatgg tcacattatt tacagcacta caatcagttt atctcattta tgtaatttat
     5641 tggattt
//
LOCUS       DAOQNP010000112         5632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_5747_length_5632_cov_8.119240, whole genome shotgun sequence.
ACCESSION   DAOQNP010000112 DAOQNP010000000
VERSION     DAOQNP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5747_length_5632_cov_8.119240"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PKY09_11665"
     CDS             <1..885
                     /locus_tag="PKY09_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13550.1"
                     /translation="LTPVACNEDGPNATTTYYPAGLTLTTTPGTVYYMIVDGFNFQGT
                     LSNGQFCVQVTNQSVIACGDTTISSGLTVLNKPVICYDSTLTIAVSGIKAPTVGTTKG
                     FSIMVSNADISGNNDPMSTGAVVGGTGTITVGSSGTFQTNLVNNTTGNPFGAGVYYIT
                     PVVYGNATGTGNITALTLDPNCTYTGTSVMVEFLAQGVICPTGIKELNGAAFAVTGIY
                     PVPVKNILNIAVESAKSTTMSISINDVLGRTVASSTQSVNNGSNTISIDTQNLSSGVY
                     TLTVNNEKQQSTVKFVKE"
     gene            complement(1137..1418)
                     /locus_tag="PKY09_11670"
     CDS             complement(1137..1418)
                     /locus_tag="PKY09_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNB13551.1"
                     /translation="MITRIVRLPVKPDEGKTFEKYFDTIKEKIAAFDGCLLVELHKDV
                     ASSDYFTYSLWQSESNLQAYLDSSFFTDVWPNAKKLLHQRANAWSLAKV"
     gene            complement(1415..2200)
                     /locus_tag="PKY09_11675"
     CDS             complement(1415..2200)
                     /locus_tag="PKY09_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNB13552.1"
                     /translation="MSLITLTTDWGLTDHYVAALKGELFSMLPGVNIVDISHQIPAYN
                     FMKAAFVFKNAFYFFPPHTIHLICVDKQLNRTDDTHTGWMVVQYGESMIVCRNNGFFT
                     LVSKQEPLKAVYIEGSEEVSVAHEKSFLVTLLHDLVHNKPLEELGQPVKSFYQSSMFQ
                     PSHDANSIQGTVIHIDIYGNAITNIEKEYFENVVGARRFEIDMPRYGETITKINNRYD
                     DVTPGKMVALFNASGFLEIALNQGDISLLYGLEYADKIRILLQ"
     gene            2374..3327
                     /locus_tag="PKY09_11680"
     CDS             2374..3327
                     /locus_tag="PKY09_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNB13553.1"
                     /translation="MSELTIPVDLENPVAFFGINDSNLDLLRKKFPAFKLIVRGNELK
                     ILGDEKQIHWVAKKIIGAIEYFHHYGKFDEQVLEKLMADGQISDALVNPPTGNGSEVL
                     VFGPNGVVVKARTANQQKMVDSCNKNDIVFAIGPAGTGKTYTAVALAVRALKNKEIKR
                     IVLTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDSLFDMIPGEKLKTFIENGVIEVAP
                     LAFMRGRTLDHAFVILDEAQNTTESQLKMFLTRMGPAAKFIVTGDVTQIDLPNKQPSG
                     LIQAMKILKNIKGIDFITLDAGDVVRHRLVKEIILAYGKNH"
     gene            3311..4051
                     /locus_tag="PKY09_11685"
     CDS             3311..4051
                     /locus_tag="PKY09_11685"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNB13554.1"
                     /translation="MVKIINWFWFLLMLAFCSCQKDHMFDCFKGTGKDKSEARNISGF
                     NRIKASNNVDVEIYPGHDYKVEVKAGSKLIDGITTEVKDSMLYIRNENACNWVRSFKN
                     KFTVSVWMPDIAELTVNGSGNIDLMDTIRHNEFTFNNWGASGKVTFLFNTEAVRTNIH
                     TGPGDFVFHGYVGVHYFYNNGNGIADASNLNTEVTFTENKGANKEYVRAKQWLTAKIS
                     YVGDIYYFGNPDSVVREGTGNGKLIKAD"
     gene            4053..>5632
                     /locus_tag="PKY09_11690"
     CDS             4053..>5632
                     /locus_tag="PKY09_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNB13555.1"
                     /translation="MRLIRNFLVLSIYWMLFFGISRIIFFLFLQFNAVNIQLSELPLT
                     LLYGIRLDISTTAYISALSLILMLIQVITGNTLFKKIFFAYNGIILFVLILLHVSNLF
                     LYRSWGVLINYRALTFLADWRQMFLSVNNVELVGGIIFIILLFLLAWFVFRKTTKHFF
                     DNKLSIIQALLPFIVTAIMTVIMFRGGVQMLPVNESAAAFSENVTLNHIATNPAWHLG
                     HNLNQAKSENAAFYKFYGDEVAEEHVKKLFQTPVDLMPVLNNKKPNIVIIMLESFTAD
                     VVGAMGGEAGISPTIDSLIHDGLSFKNIYSSGARTDQAFVAVLSGFPAQPNQSIMRFP
                     DKTAKLPSLPAELKKQGYNTSFYYGGDLDFSNLNTYLVNCGFEHITGKKKFKQQQLSA
                     KWGAHDEFVFEKNLNDLKQSKEPFFSVVMTLSSHEPYDIPMKTPFGKANANEQFKGSA
                     WYADHSLGNYLAAAKKEPWFKNTLFVVVADHGNKIPRDRSYYSHYIRHIPMVMAGDVV
                     KNIYKNKIVYSTGSHHDIA"
BASE COUNT         1802 a         1026 c         1041 g         1763 t
ORIGIN      
        1 cttactcctg ttgcttgtaa tgaagatggt ccgaatgcca caacgactta ttatcctgca
       61 ggcttaacct taaccactac tccaggtaca gtttattata tgattgttga tggatttaat
      121 tttcagggta cactttcaaa cggacaattc tgtgttcagg taactaatca atcagtaatt
      181 gcatgtggtg acactacaat ttcatcaggt ttaactgtat tgaataaacc tgtaatttgc
      241 tacgacagca ctttaacaat tgctgtttcc ggaattaaag cacctacagt tggtacaact
      301 aaagggttca gtataatggt ttcaaatgca gacatcagtg gtaataatga cccaatgtca
      361 acaggtgctg tagtaggtgg aacaggaact attaccgttg gaagcagcgg tacattccaa
      421 acaaatttag ttaacaacac aactggtaat ccttttggtg ctggtgtata ttacattaca
      481 cctgtagttt atggtaatgc aacaggaaca gggaatatta cagcactgac acttgaccct
      541 aattgtactt atacaggtac atccgttatg gttgaattct tagcacaagg tgttatctgc
      601 ccaacaggta ttaaagaact taatggtgca gcttttgccg taacaggaat ttatccggta
      661 ccagtaaaga acatattgaa catagctgtt gaatctgcaa agtcaacaac aatgtcaatt
      721 tcaattaatg atgtacttgg aagaactgta gcttcaagca cacaatctgt aaacaatggc
      781 agcaacacca tttctataga tacacaaaac ctttcatcag gagtttatac acttacagta
      841 aacaatgaaa aacagcaatc aactgttaaa tttgtaaaag aataattcag attgtttgaa
      901 cctaaaaaaa ggctgcctcg taaaggcagc ctttttttat gagcagaggt aaatattttt
      961 attgcttctc ttcgttatta aaactttatt gtcattccat ttttcttttc cttttgagat
     1021 aatgaatagt ttaagttccg tttgctatcg gatggatgga ctttggcagc aactattaat
     1081 gacaaactac ttgactatgc attatcttaa gaataccatt acaaactttc aatctttcaa
     1141 actttggcaa gcgaccatgc attggcacgt tgatgcagca acttcttagc attaggccaa
     1201 acgtctgtaa aaaacgatga atcaagatag gcttgtaaat tgctttcact ttgccaaagg
     1261 ctgtaggtaa aataatcaga agaagccaca tctttatgta actcaaccaa caaacatccg
     1321 tcaaaagctg caattttttc tttaattgta tcaaaatatt tttcaaaagt tttaccctca
     1381 tcgggcttaa caggcaatct tacaatacgt gttatcattg caacaaaatt ctgattttat
     1441 cagcatattc aagtccgtac aacaatgaaa tgtctccctg atttaatgca atttcaagaa
     1501 agcccgatgc attaaacaaa gccaccatct ttccgggagt tacatcatca taacgattat
     1561 ttattttagt aattgtttcc ccgtagcgag gcatatcaat ttcaaatcgc cttgctccta
     1621 ccacattttc aaaatattct ttctctatat tggtaatggc attgccatag atatcaatat
     1681 gaataaccgt tccctgaatt gaattggcat cgtgtgatgg ctgaaacatt gacgactgat
     1741 aaaaagattt cacaggctgt cctaattctt ccaaaggttt attatgtacc aaatcatgca
     1801 acagtgtaac caaaaaactt ttctcatgtg ccacactcac ctcttctgaa ccttcaatgt
     1861 aaaccgcttt taaaggctcc tgcttactta ctaacgtaaa aaaaccatta ttgcgacaaa
     1921 caatcatact ttctccatat tgaacaacca tccagcctgt gtgtgtgtca tctgttctat
     1981 tcaactgttt gtccacacag attaaatgaa ttgtgtgagg aggaaaaaag taaaatgcat
     2041 ttttaaaaac aaaggcggct ttcataaaat tataagcggg aatctgatgg cttatatcaa
     2101 caatattcac ccccggcagc atgctgaaca attcgccctt tagcgctgct acatagtgat
     2161 cggttagtcc ccaatctgtt gtgagtgtaa ttaatgacat gaatactgtc ggaattaatt
     2221 ccacaaaaat attcgtactg ctgtaagcac atctttctgc atatacaaat tataaacttt
     2281 ctgatgttag tgaagattgc cggaatttta ataaattcaa ctgctgatat gtctaaaaaa
     2341 aattaggttt gcatacatta aaccattgta caattgagtg agttaaccat acctgttgat
     2401 ttagaaaatc ctgtagcgtt ttttggcatc aacgatagta atttagattt attaagaaaa
     2461 aaattccctg cttttaagct gattgtcaga ggtaatgagc ttaaaatttt gggtgatgaa
     2521 aagcaaattc attgggtagc aaaaaaaatt atcggtgcaa ttgaatactt tcatcactac
     2581 ggaaaatttg atgagcaagt gctggaaaaa ctaatggctg atggacaaat ttctgatgct
     2641 ttggtaaatc caccaaccgg aaatggcagc gaagttttag tcttcggacc aaatggtgtt
     2701 gttgtaaaag cacgtactgc caatcaacaa aaaatggttg acagctgcaa taagaacgac
     2761 attgtgtttg ccataggtcc tgccggaacc ggaaaaactt atactgccgt agcattagct
     2821 gtacgtgcat taaaaaataa agaaatcaag aggattgttc tgactcgtcc tgctgttgag
     2881 gccggagaaa accttggatt tttacccggt gacttaaaag aaaaaattga cccttatctg
     2941 cgtccgcttt acgattcatt gtttgatatg attcccggtg agaagttaaa gacctttatt
     3001 gaaaacggag ttattgaggt tgcacctttg gcttttatgc gtggccgcac gttagaccat
     3061 gcattcgtta ttcttgacga agcacaaaat accacagaaa gtcagctaaa aatgttcctt
     3121 acaagaatgg gacctgctgc aaaatttatc gtcacaggcg atgtaacgca aattgactta
     3181 cccaacaaac aaccatcagg gttgattcag gcaatgaaga ttctgaaaaa cattaaaggc
     3241 attgacttta ttacactcga tgcaggtgat gttgttcgtc atcgtttggt taaagagatt
     3301 attctggcct atggtaaaaa tcattaattg gttttggttt cttctgatgc ttgcattctg
     3361 ttcttgtcag aaagaccaca tgtttgattg ctttaagggt acaggaaaag acaaatcaga
     3421 agcaagaaac atatcaggat ttaaccgtat aaaagcaagc aacaatgtag atgtagaaat
     3481 ttatccgggt catgattata aagttgaagt aaaggcgggt tcaaaattga ttgatggcat
     3541 caccacagaa gttaaagaca gcatgctgta cattagaaac gaaaatgcat gtaactgggt
     3601 tcgtagtttt aaaaataaat ttaccgtttc tgtttggatg cctgatattg cagagcttac
     3661 agtaaatggt tcaggcaata ttgacttgat ggacactatt cgtcataatg agtttacgtt
     3721 caacaattgg ggtgcatcag gcaaggtgac ctttctgttt aacacagagg cagtgagaac
     3781 caacatacat accggacctg gcgatttcgt ctttcatggt tatgttggtg tacattattt
     3841 ttataataac ggtaatggca ttgccgatgc aagcaacctg aatacagaag ttacttttac
     3901 agaaaacaaa ggtgctaaca aagaatatgt cagagctaaa caatggctca cagcaaaaat
     3961 ttcctatgtg ggtgatattt attacttcgg taaccccgac agcgtagtgc gtgaaggtac
     4021 tggaaatgga aaattaatta aagcagatta gcatgcgctt gattagaaat tttctagttc
     4081 tgtcaattta ctggatgttg ttcttcggaa tttcaagaat tatctttttc ttatttcttc
     4141 aatttaatgc tgtaaatatt caactgagcg aattgccatt aacattactt tatggtatcc
     4201 gattagatat ttcaacaact gcatacattt cggcactgtc gttaatctta atgctgattc
     4261 aagttattac aggtaacact ttatttaaaa aaatattttt tgcgtacaat ggaattattc
     4321 tatttgtttt aattctcttg catgtcagca acctttttct ctatcgttca tggggtgtac
     4381 ttattaacta tcgtgcactg acttttcttg cagactggcg gcagatgttt ttatcagtga
     4441 ataatgttga attggttggc ggaataatat tcattatact tttatttctt cttgcatggt
     4501 ttgtattcag aaagactaca aaacactttt ttgacaacaa actttcaatt atccaagcat
     4561 tacttccatt tattgtcaca gcgataatga ctgtaatcat gtttcgtggt ggtgttcaaa
     4621 tgttgccggt aaatgagagt gcagctgcat tcagtgagaa tgtaacgctc aatcacattg
     4681 ctacaaatcc tgcctggcat ttgggacaca atctcaatca agccaaaagt gaaaatgctg
     4741 cattctataa attttatggc gatgaagttg ctgaagagca tgtaaaaaaa ctgtttcaaa
     4801 cccctgttga tttaatgcct gtgctgaaca acaaaaaacc aaacattgtc attatcatgc
     4861 tggagagttt tactgctgat gttgttggag ctatgggcgg tgaagcaggt atttctccaa
     4921 caatagattc attgattcac gatggattat cttttaaaaa catttacagt agtggtgcac
     4981 gcaccgatca ggcatttgtg gcagtattga gtggatttcc tgcacagcct aatcaatcta
     5041 tcatgcgttt tcctgataag actgctaagc ttccctcctt acctgccgag ttgaaaaagc
     5101 aaggctataa cacttctttc tattacggtg gcgatctcga tttttcaaac ctgaatacct
     5161 atttagtcaa ctgtggattt gaacacatta ccggcaaaaa gaaattcaag caacagcaac
     5221 tgagtgcaaa gtggggagcg catgatgaat ttgtttttga aaaaaacctg aatgatttaa
     5281 aacaatcaaa agaaccattc ttttctgttg tgatgacatt aagctcgcat gaaccctacg
     5341 acattccaat gaaaacacct tttggaaaag ccaatgcgaa cgagcagttt aagggatctg
     5401 cctggtatgc agaccatagt ttgggaaatt atcttgctgc tgccaaaaaa gaaccgtggt
     5461 ttaaaaatac tttatttgtt gtagttgctg atcatggtaa taaaatacct cgtgacagat
     5521 catactacag tcattacatc agacacattc ccatggtaat ggctggcgat gttgtgaaaa
     5581 atatttataa gaataaaatt gtttacagca caggttcaca tcatgatatt gc
//
LOCUS       DAOQNP010000113         5521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_5911_length_5521_cov_6.188621, whole genome shotgun sequence.
ACCESSION   DAOQNP010000113 DAOQNP010000000
VERSION     DAOQNP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5911_length_5521_cov_6.188621"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKY09_11695"
     CDS             complement(<1..869)
                     /locus_tag="PKY09_11695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNB13556.1"
                     /translation="MQSLQKPVPVSVRINPKKHTEHISVSSVPWCSQGYFLEQRPVFT
                     LDPLFHAGAYYVQEASSMFLEQVIVQLNLNKKPINILDACAAPGGKTTHLLSLLHPES
                     LVVANEVIAPRAPVLVENAIRWGYSNLVVTRGDVGLFKRLANFFDVVVCDAPCSGEGL
                     FRKDVEAISEWSLQNVEHCSLRQQRIVNDLWDALKPGGYFIYSTCTSNLQENEINVQH
                     FIDKYNAESIKLNIKNFSGLEEFQRQDVVMYRFFPHKTGTEIFSIAVLKKPDLNCMNS
                     ERVAAKTLKLADKV"
     gene            1074..2927
                     /gene="yidC"
                     /locus_tag="PKY09_11700"
     CDS             1074..2927
                     /gene="yidC"
                     /locus_tag="PKY09_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNB13557.1"
                     /translation="MDRNSILGVVLIMAVLIGFSIFNQPSKEEQEAAKRKQDSLALVE
                     KKYQDSLKLVTKPVAALKADTVAVSDTLNNDSLAQTKLTDNFGVFGLAAQGEHKSITL
                     ESDLLKLKLNSKGGQIDYVELKKYKTGEGKPLVLNNGDSSQFNLSFFSENKMISTGDL
                     YFELPAKGITVTGKDSQSVSIKLNAGEGRYIEYVYSLKGDDYRVGFKINFVNMGSLVA
                     SNAGYMELNWKQKLLQQEKSHQNESAVSTIYYRYTDEEVDHLSETKDDGTELKTKVQW
                     VGFKQQYFSSVLIADQAFESPTRINSTVIANEGAVRMMHANFTIPFSHQNNESTGMSF
                     YYGPNHYQTLKKYNIGLEKLVPLGWGIFGWVNRFAVIPVFNFLNSFDINYGIIILILT
                     ILIKVVLLPLTYKSYISTAKMKVLQPEMSEIQAKFKSDPMKLQQETMTLYRKAGVSPL
                     GGCLPMLLQMPILIAMFRFFPASIELRQEGFLWAKDLSTYDSILDLPFNIPFYGDHVS
                     LFTILMTVSTLIYTRMNMQMTAATNPSMKYVMYLMPIMFLGIFNNYSSGLSYYYFLAN
                     MITFGQQYLFKSFVDEKAIHAKIQENKKKPVKKSKFQERLEKMAKERGVRK"
     gene            3150..3857
                     /locus_tag="PKY09_11705"
     CDS             3150..3857
                     /locus_tag="PKY09_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNB13558.1"
                     /translation="MNKVVKNVDEALKDFRDGMTLMVGGFGLCGIPENCIAALVRKGI
                     KGLTCISNNAGVDDFGLGLLLQQKQIKKMIASYVGENAEFERLMLTGEMEVELIPQGT
                     LATRCLAAGYGMPAIFTPAGVGTEVAQGKEVRRFSYNGVEKDYLMEYAFDSDAALVKA
                     WRGDTEGNLVYRSTARNFNPMMAMAGKITIAEVEELVPAGTLDPDQIHTPGIYVHRIF
                     QGEKYEKRIEQRTVRKK"
     assembly_gap    3932..4031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4105..4470
                     /locus_tag="PKY09_11710"
     CDS             4105..4470
                     /locus_tag="PKY09_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNB13559.1"
                     /translation="MSRKFNTPFEDNTILKLTFEFSLKIIHLSEVLNSNHQYIISKQI
                     LKSATSIGANSYEAQNAESDADFIHKFKIAAKEADETHYWLLLCNHADNYPDTTNLLN
                     ELEIIKKVINRIISTSKNK"
     gene            4593..5249
                     /locus_tag="PKY09_11715"
     CDS             4593..5249
                     /locus_tag="PKY09_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HNB13560.1"
                     /translation="MLDKNSIAKRIAREVKDGMYVNLGIGIPTLVANYIPKGINVTLQ
                     SENGILGMGPFPFEGEEDADLINAGKQTVTLLPGSVIFDSAMSFGMIRAQKVDLTILG
                     AMEVSEEGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLPKCTLP
                     LTGIKCIKRIVTELAVLDVTPQGFKLLERAPGVSVEEIKKATAGKLIVEGDIPEMVID
                     "
     gene            5262..>5521
                     /locus_tag="PKY09_11720"
     CDS             5262..>5521
                     /locus_tag="PKY09_11720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNB13561.1"
                     /translation="MRYLDHFEKIAEKRIRDRHKHRYYWNDITFYCNYFSHETFSVLE
                     VGCGTGELLNNIRAARKTGIDYSPIMITEAKKQFPQIDFQVMA"
BASE COUNT         1829 a          921 c         1029 g         1642 t
ORIGIN      
        1 actttatcag ctaacttcag agtctttgca gctactcttt cagaattcat gcaatttaaa
       61 tcgggctttt ttaatactgc tattgaaaaa atctctgttc ctgtcttatg tggaaagaag
      121 cgatacatga caacatcttg tctttgaaat tcttctaatc cggaaaaatt tttgatatta
      181 agttttatac tttcagcatt atacttatca ataaaatgct gaacattgat ttcattctcc
      241 tgtaaatttg atgtgcaggt tgagtaaata aagtaacctc ccggttttaa ggcatcccat
      301 aaatcattta caattcgttg ttggcgcaaa gaacagtgtt caacattttg cagactccat
      361 tcacttatag cttccacatc ttttctgaac aacccttctc ctgaacaagg tgcatcgcaa
      421 acaacaacat caaaaaaatt tgccaagcgc ttgaaaagtc caacatctcc acgtgtgaca
      481 actaaattgg aatagcccca ccgtatagca ttctctacaa gaacaggtgc ccggggtgca
      541 ataacttcat ttgccacaac aagagactca ggatgcaata acgacaataa atgtgtagtt
      601 tttcctcccg gtgctgcaca ggcatcaagt atatttattg gtttcttatt gaggttcagc
      661 tgtacaatta cctgctctag gaacatgctt gatgcctctt gaacataata cgcccctgca
      721 tgaaacaatg ggtctaaagt aaaaacaggg cgctgctcca gaaaataacc ttgactgcac
      781 catggcacag aactaactga aatatgttca gtatgctttt tgggatttat cctcactgac
      841 accggtacag gtttttgtaa tgactgcaca aatagcactt tttctgattc agaaagaggc
      901 aacgattcca gaaattcttt tggcaacaac aagaatatga ttatataagt tgtggttgta
      961 aaaatacatg aatcttcaca gttttatatc actctgagtt acagtataat tctgaatttt
     1021 acaaagccta ctcctatgct tcatttatta tctttgccgt gttttttaaa aatatggata
     1081 gaaattcgat tttaggagta gtgttaatta tggctgtgtt gattggcttt tcaatattca
     1141 atcaaccttc aaaagaagag caggaagcag caaaacgaaa gcaggactct cttgcattgg
     1201 ttgaaaaaaa gtatcaggac agtttaaaac ttgttaccaa acccgtagct gcactgaagg
     1261 ccgatacagt agcagttagc gacacactaa acaatgactc acttgctcaa acaaaactta
     1321 cagacaattt tggagtgttt ggcttagcag cacaaggtga acacaaaagt attactctgg
     1381 aaagcgattt gttgaaatta aagctcaata gtaaaggtgg acagattgac tatgtagaat
     1441 taaaaaaata taaaaccggt gaaggaaaac ctttggtgct gaacaatgga gatagctctc
     1501 agttcaacct tagctttttt tcagaaaaca aaatgatttc tacgggagat ttgtattttg
     1561 aattacctgc aaaaggaatc actgtgaccg gaaaagactc tcagtctgtt tctattaagt
     1621 taaatgccgg tgaaggtcgc tatattgaat atgtttattc attaaaaggt gatgattatc
     1681 gtgttggctt taaaatcaac ttcgttaaca tgggctcatt ggtggcttcc aatgcaggat
     1741 atatggagtt aaactggaaa cagaaactac tgcaacaaga aaaaagccat caaaatgaaa
     1801 gtgctgtttc aacaatttac tatcgctata ctgacgaaga agttgaccat ttgagcgaaa
     1861 ccaaagatga tggaacagaa ttaaaaacaa aggttcaatg ggtaggattt aagcaacaat
     1921 atttttcaag tgtattgatt gctgaccagg cttttgagag tccgacaaga attaacagta
     1981 ctgtaattgc taatgagggt gctgtgagaa tgatgcatgc caatttcact attccattca
     2041 gtcatcagaa taatgaaagc acaggcatga gtttttatta tggtcctaat cactatcaaa
     2101 cactaaaaaa atacaacatc gggctggaga aacttgttcc gttaggatgg ggaatttttg
     2161 gttgggtaaa tcgttttgct gttattcctg tgtttaactt tctcaacagt tttgatatta
     2221 actatggtat catcatacta attttaacaa tactaatcaa agtggtgtta ttgcctttaa
     2281 cctataagtc atacatcagt actgcaaaaa tgaaagtgtt gcaaccggaa atgtcggaga
     2341 tacaagctaa gttcaaatct gacccaatga agttgcaaca agagaccatg actctttacc
     2401 gaaaagccgg agttagtcct ttaggcggtt gcttaccaat gttgttgcaa atgccaatat
     2461 taattgccat gttccgtttc tttcctgcat caattgagtt acggcaggaa ggttttttat
     2521 gggcgaaaga tttaagtact tatgattcta tacttgattt acctttcaac attccttttt
     2581 atggcgacca tgtgagttta tttacaattc tcatgactgt atcaacactc atctatacac
     2641 gcatgaacat gcaaatgact gctgctacaa acccttcaat gaaatatgtg atgtacctga
     2701 tgccgatcat gtttcttgga attttcaaca actattcttc tggattgagt tattactact
     2761 ttctggctaa catgattacc ttcgggcaac aatacctctt taaatctttt gttgatgaaa
     2821 aagcaattca tgcaaaaatt caagagaata aaaagaagcc tgttaaaaaa tcaaagtttc
     2881 aggagcgatt agaaaaaatg gctaaagagc gtggcgtaag aaagtaaaca gtctgaaagt
     2941 tgtaaagtag aaatgtttgg ttgttttaag gaaagaatat ctgagtaatg caatgatttc
     3001 atgataaaca gtaaagtctt gttgattata tttaaatttg aaattttatt attctaaact
     3061 ttactttgca agagtcagtt taaaagcatt tttaatttag accaacaaat caataactta
     3121 aatttcgaat taataaattt ttaaataata tgaataaagt tgtaaaaaat gttgacgagg
     3181 ctttaaaaga ttttcgtgat ggcatgacac taatggttgg tggctttgga ctttgcggca
     3241 ttcctgaaaa ctgtattgca gccttagttc gtaaaggcat taaaggacta acctgtattt
     3301 ctaacaatgc aggtgttgat gattttggtt tgggattact cttgcagcaa aagcagatca
     3361 aaaaaatgat tgcaagttat gttggagaaa atgcagagtt tgaaagactg atgctaacag
     3421 gagaaatgga ggtagaatta attccacagg gaactcttgc cacccgatgt ctggcagcag
     3481 gctatggcat gccggcaatt tttacacctg ccggtgtagg tacagaagtt gcacaaggga
     3541 aagaagtaag acgtttcagt tacaatggcg ttgaaaaaga ttatctgatg gagtatgcat
     3601 ttgattctga tgctgcattg gtaaaagcct ggagaggtga cacagaagga aatcttgttt
     3661 accgttcaac agcgcgaaat tttaatccaa tgatggctat ggcaggaaaa attaccattg
     3721 cagaagtaga agaattagtc cctgcaggaa cacttgaccc cgatcaaatt cacacaccgg
     3781 gaatttatgt acaccgtatt tttcagggtg agaagtatga gaaaagaata gaacagagaa
     3841 cggttaggaa aaaataattt aatgatgaga taatttgatg actattgaga taatttgatg
     3901 actattgaga taatttgatg actattgaga tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn ngatgactat tgagataatt tgatgactat tgagataatt tgatgactat
     4081 tgagataatt tgatgactat tgagatgtcg agaaaattta atactccttt tgaagacaat
     4141 acaatactga aactcacttt tgagttttca ttaaaaatta ttcacctgtc tgaagtttta
     4201 aattccaatc atcaatatat tatttcaaaa caaattctaa aatcagcaac ctccattggg
     4261 gctaactcat acgaagcaca aaatgctgaa agcgatgccg attttattca taaatttaaa
     4321 attgcagcca aagaagctga tgagacacat tactggcttt tgttgtgtaa tcatgctgat
     4381 aattatcctg acacaactaa tttactgaat gaattagaaa ttataaaaaa agtaatcaat
     4441 agaattattt caacctcaaa aaataaataa taaaatatta tcttagagac atttcaacat
     4501 catattactc catatacaac gtccacgaaa gtcgcatact acaatcggct ttcattatca
     4561 aattatctaa ttttcaaatc atctaattaa atatgctcga caaaaattca atagcaaaac
     4621 gcatagcccg cgaagttaaa gacggcatgt acgtaaacct tggcattggc attcccacat
     4681 tagtagccaa ttatattccc aagggaataa atgtaacgct acaatcagaa aacggaattc
     4741 ttggcatggg tccattccct tttgaaggtg aagaagatgc agacttgata aatgccggta
     4801 aacaaactgt tacattactt cctggttccg taatctttga ttcagcaatg agttttggga
     4861 tgattcgcgc tcagaaagtt gatttaacaa ttctaggtgc aatggaagtg agtgaagaag
     4921 gagatattgc caactggaaa ataccaggca agatggttaa aggtatgggt ggtgctatgg
     4981 acttagtagc atcagcgaaa aatattattg tggccatgca gcatgttaac aaagccggag
     5041 agtcgaaact tttacctaaa tgcactttac ctctgacagg aattaaatgt ataaaaagaa
     5101 ttgttactga gcttgctgtt cttgatgtta ctcctcaggg ctttaaactt cttgaacgtg
     5161 ccccgggtgt ttctgttgaa gaaattaaaa aagctactgc cggtaaactt atcgttgaag
     5221 gcgatatccc cgaaatggtg attgattaac atcaggcatt gatgagatac ctcgatcatt
     5281 ttgaaaaaat tgcggagaaa agaattcgcg acaggcataa acatcgttat tactggaacg
     5341 acataacatt ttattgtaac tacttttcgc acgaaacttt ttctgtatta gaagtaggct
     5401 gtggcacagg tgagctatta aacaacatcc gtgctgcccg taaaaccggc attgactaca
     5461 gccctataat gattacagag gcaaaaaaac agtttccgca gattgacttt caggtaatgg
     5521 c
//
LOCUS       DAOQNP010000114         5405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_6132_length_5405_cov_5.915140, whole genome shotgun sequence.
ACCESSION   DAOQNP010000114 DAOQNP010000000
VERSION     DAOQNP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6132_length_5405_cov_5.915140"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PKY09_11725"
     CDS             complement(<1..983)
                     /locus_tag="PKY09_11725"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNB13562.1"
                     /translation="MKLLTKEQFRLADAYTIKTEPISSVDLMERAATSCVDVITKFTD
                     EKDLICILCGPGNNGGDGLAIARMLAESGKNVSVILTDKSDKLSPDAATNFQRLQKTS
                     VNFFYLTDTQLPFEKITVWIDAIFGTGLNKVVEKNIADLIHKVNSTGATIFSIDLPSG
                     LFADKHTPASAGVIHASTTLTFQQLKRCMLYAENAHRIGEVKVLDIGLDEHYIESLTS
                     THELVDRSMIKKSLKPRKRFSHKGNFGHALLIGGSYGKAGAIVLSAKACVRSGAGLTT
                     VHTIKTTGAALQSAMPEVMLSTCEGDKVFALPDLTSYNAIAAGPGLGIYEET"
     gene            1103..2203
                     /gene="gcvT"
                     /locus_tag="PKY09_11730"
     CDS             1103..2203
                     /gene="gcvT"
                     /locus_tag="PKY09_11730"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNB13563.1"
                     /translation="MEENTTLKHTALTAIHQKAGAKMVPFAGYLMPVQYTGINDEHET
                     VRNAVGMFDVSHMGEFILKGEDALDLLQRVTSNDASKLSAGKAQYSYFPNETGGIIDD
                     LIVYCIDDKTFMLVVNASNIEKDWNWLQKHNNKKVEMHNISDQTSLLAIQGPKALETL
                     QKLTSITLADIPYYNFAKGEFSSCQNVLISNTGYTGAGGFELYFENQYAEKIWNDIMQ
                     AGQPFGIKPAGLGSRDTLRLEMGFCLYGNDISDNTSPLEAGLGWITKFTKNFINSDAL
                     KQQKEQGIANKLVGFEMIDRGIPRHDYEICNASGQKIGRVTSGTQSPTLNKAIGMGYV
                     AKDQSAEGTEIFIKIREKLIKAAVVKIPFIKK"
     gene            2207..2944
                     /locus_tag="PKY09_11735"
     CDS             2207..2944
                     /locus_tag="PKY09_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNB13564.1"
                     /translation="MSEIVGKPVVEVCLTPALFHQYNTKDSIVVAIDILRATSSICVA
                     LQYGARSIVPVMTVEESFQFKEKGFLIGAERQGSMVEGFDLGNSPFSYMEDKIVGRDI
                     ALTTTNGTKAINIAKGSAGIVAGSFLNIDVLAAWLIQQNKNVLCLCAGWKNTFNLEDT
                     LFAGALTQKLIPHFQLSEHRDSAIAAINLYNLAKHDMYDFLKDSSHRARLERLKIDED
                     VLYCLTPNQTLVVPVLKDGVLKKHSDW"
     gene            3057..3395
                     /locus_tag="PKY09_11740"
     CDS             3057..3395
                     /locus_tag="PKY09_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13565.1"
                     /translation="MKKALSVLSVLGIFLIVNAYANSNKANTVTDLQVTECNIMASHD
                     MPCDGKAEKDKDCCKKKSKKKKCCTDKAEASAASCADKQAKAGCCAHKKADAGASDVK
                     TENKSEESAK"
     gene            complement(3525..4979)
                     /locus_tag="PKY09_11745"
     CDS             complement(3525..4979)
                     /locus_tag="PKY09_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13566.1"
                     /translation="MKTKSNVLILLLFCSVSVFGQNLIWNPSVKLNERPYQKFLGTNN
                     GYFYAAGSNIKMIQDISDSIFLTTAWKKLKLTLLRYDDKFILKDTAAIRFLQSPVRVY
                     DVVLSDSTIKVFYSKGIDNLEFCADIFDLRGYFKQTIVLITGKDVVKFNQKSFFNYNT
                     SINKHYFTISTNDSIYCFSNQLNNVWKTVLPNSTFFDGTVNDNGTFSGVFSSGKEHFI
                     ATCVDGKVITQTISTEKAELADFKIKHEGNRIIVASLFGFTDNTFNPIYNNIANAKKF
                     KSNGVSISEFNNDLQLIKTNSLKFKDETLLETVDNLLLKNIKGVDFLKIQQIDVMSNG
                     NIMILIEKLFSSYTKPAAVNGKTNTIIGRETSANELLLGCTDMQNKNLQTVIKRKVSA
                     TENFEYVCNVKGIPHKDDYYLYFLDGSNPDAYQITQTIFNSELRKIYTKELELASAKK
                     LLPDLSTITRISNSRYILNARLLKKFSTAILDFK"
     gene            complement(4963..>5405)
                     /locus_tag="PKY09_11750"
     CDS             complement(4963..>5405)
                     /locus_tag="PKY09_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010601302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNB13567.1"
                     /translation="QPTHATSDMYWAEKRLGSVRIHDAYSNNALLKETGMLALGSDFP
                     VEDINPLYGFYAAVVRKDQKGFPPEGFEIENSISRTEALRGMTIWAAYSMFEENEKGS
                     LEKGKAADFVIMNDDLMTAPDSVLFKLPVEATYSNGVKVYENKK"
BASE COUNT         1704 a          965 c         1024 g         1712 t
ORIGIN      
        1 gtttcttcgt aaatgcccaa gcccggtcct gctgcaattg cattgtagga tgttaagtca
       61 ggtaaagcaa atactttatc accttcacaa gtacttaaca ttacctccgg cattgcagat
      121 tgtagtgccg cacctgttgt ttttatggta tgaactgttg tcagccctgc accacttcta
      181 acacatgcct ttgcggaaag tactatagct ccggcttttc cataactgcc gccaatcaat
      241 aaagcatgac caaagttgcc tttatgcgaa aatcgttttc tcggcttgag tgattttttt
      301 atcatgctcc tgtcaaccag ttcatgagtt gaagttaatg attcaatata atgttcatca
      361 agcccaatgt caagcacttt tacttcgcca atcctatggg cgttttctgc ataaagcatg
      421 cagcgcttta attgctgaaa agttaatgtt gtggaagcat gaataacgcc agcactggct
      481 ggtgtatgtt tgtcggcaaa taatcctgaa ggtaaatcaa tagaaaaaat agtagcccca
      541 gtggaattca ctttgtgaat taagtcagca atatttttct ctacaacttt atttaatcct
      601 gttccgaaaa ttgcatcaat ccaaacagtt attttttcaa aaggaagttg ggtgtcagta
      661 aggtaaaaaa agttgacaga agttttctgc aatcgttgaa aatttgttgc tgcatcgggg
      721 cttaatttgt cgcttttatc ggtaagtata acagaaacat tttttcctga ttcagccaac
      781 attctggcaa tggcaagtcc atcacctcca ttatttccgg gtccgcagag aatgcaaatc
      841 agatcttttt catctgtaaa ttttgttatt acatcaacgc agcttgtggc tgctctttcc
      901 attaaatcaa cagagctaat aggttctgtt tttattgtat aggcatcggc caaacggaat
      961 tgttctttag ttaatagttt catctgtctg tcaggcagta gtggagattg gtgtaaagat
     1021 aaaaaaccgg tattgaaata atatgaatca tattgaacaa gcagtaaaac atgtagattt
     1081 gccgcacatt ttaaagaata gtatggaaga aaataccacc ttaaaacaca cagctttaac
     1141 agcaattcat caaaaggcag gtgctaaaat ggtgcccttt gcaggctacc tgatgccggt
     1201 gcaatatacc ggaattaatg acgaacatga aactgttcgc aatgccgttg gcatgtttga
     1261 tgtctctcac atgggagaat ttattttaaa aggcgaagat gcactcgatc ttcttcaacg
     1321 tgttacaagc aatgatgctt ctaaattgtc agcaggtaaa gctcaataca gttatttccc
     1381 aaatgaaaca ggtggaatta ttgatgattt aattgtttat tgtattgatg ataaaacatt
     1441 catgttagtt gttaatgcat ccaatattga aaaagactgg aattggcttc aaaagcataa
     1501 caacaaaaaa gttgaaatgc ataacatctc tgaccaaact tcattgcttg ctattcaggg
     1561 gcctaaggca ttggaaactt tgcagaagct gacttcaata accttagccg acattccata
     1621 ttacaacttt gctaaaggtg agttttccag ctgccaaaat gtgcttatct ccaatacagg
     1681 ctacacaggc gcaggtggtt ttgaattgta ttttgaaaac cagtatgcag aaaaaatctg
     1741 gaatgacatt atgcaggctg ggcaaccatt cggcataaaa cctgcaggac tgggttcgcg
     1801 tgacaccttg cgacttgaaa tgggattctg tttatacgga aatgatatta gtgacaacac
     1861 atcgcctttg gaagcaggac ttggatggat taccaaattc acgaagaatt ttatcaactc
     1921 tgatgcttta aagcaacaaa aagagcaggg aatagcaaat aaattggtag gctttgaaat
     1981 gattgatcgt ggaattccaa gacatgatta cgaaatatgt aatgcatcag gacaaaagat
     2041 tggtcgtgtt acttcaggaa cacagtcacc aacattaaac aaagccatcg gtatgggcta
     2101 tgtggcgaaa gatcaatctg ccgaaggaac agaaattttt attaagatca gagagaagct
     2161 gattaaagcc gcagttgtca agataccgtt tattaaaaaa taatcaatgt ctgaaatagt
     2221 gggcaaacct gtagttgaag tttgtttaac tccggcactt ttccatcagt acaacactaa
     2281 agattctatt gttgtagcaa ttgatatttt acgtgctaca agtagtattt gtgtggcatt
     2341 gcaatatggt gcacgctcca ttgttcctgt aatgacagta gaagaaagtt ttcagtttaa
     2401 agaaaaagga tttctgatag gagcagagcg gcaaggctca atggtagaag gtttcgacct
     2461 cggtaattcg ccattcagtt atatggaaga caagatagtc ggtcgcgata ttgcactaac
     2521 cacaaccaat ggcactaagg caattaatat tgccaaaggc tctgcaggaa ttgttgcagg
     2581 ttcatttctg aatattgatg tgcttgctgc ctggcttatt caacagaata aaaatgtgct
     2641 ttgcctgtgt gcaggttgga aaaacacttt taatcttgaa gatacacttt ttgcaggagc
     2701 actgacgcaa aaacttattc cacattttca actgtctgaa caccgcgatt ctgcgattgc
     2761 agcaataaac ctttataacc ttgcaaagca tgatatgtac gattttttga aagactcttc
     2821 acatcgtgca cggttggaaa gacttaaaat tgatgaagac gtattgtatt gcctgacacc
     2881 aaatcaaacc cttgtagttc cggtgttaaa ggatggggtt ttaaagaaac actccgactg
     2941 gtagtaaaat cattgctttt acagatggtt atttaatgaa aaagttgtgc cggtgactta
     3001 ggtcagttgt attataggac tgaaaatact actttagcag aaaattattt agaaaaatga
     3061 aaaaagcact ttcagtttta agcgtactcg gaatattcct gattgttaac gcttatgcaa
     3121 acagcaacaa agcaaacaca gttactgacc ttcaggtaac agaatgcaac attatggcaa
     3181 gtcatgacat gccttgtgat ggtaaagcag aaaaagataa agattgctgc aaaaagaaaa
     3241 gcaaaaagaa aaaatgttgc acagacaaag ctgaagcttc agcagcatca tgtgccgaca
     3301 agcaagcaaa agcaggttgc tgtgcacata aaaaagctga tgccggtgct tctgatgtaa
     3361 aaacagaaaa taagtcagaa gaatctgcaa aataatttta tcatgccatg gggtgcagta
     3421 tttagcttgt aaagctgtgt aatacccttc ggttttaaga tgaaaattga aaaccgttct
     3481 ctttattgag aacggttttt ttattagcta attctaagct gtttttattt gaaatctaaa
     3541 atggcagtag aaaatttctt taatagtctt gcattcagaa tatacctgct attgctaata
     3601 cgagttatgg ttgacaaatc aggcagaagt ttttttgccg aagctaattc taattctttt
     3661 gtgtaaattt tcctgagttc agaattaaag attgtttggg taatctgata tgcgtccgga
     3721 ttgctgccat ctaaaaaata aaggtagtag tcatctttat gtggaatgcc tttgacattg
     3781 catacatact cgaaattttc tgttgcactt acttttcttt taataacagt ctgcaaattt
     3841 ttattttgca tatccgtaca acccaaaagc agttcattgg ctgatgtttc ccttccaatt
     3901 atcgtatttg ttttaccatt cacagcagca ggtttagtgt aggatgaaaa cagcttttca
     3961 attaaaatca taatgttgcc attgctcatt acatcaattt gctgaatctt taaaaagtca
     4021 acacctttga tgttttttag taataagttg tcaaccgttt caagcaatgt ttcgtctttg
     4081 aatttcaaac tatttgtttt aataagttga aggtcattat tgaattccga aatacttaca
     4141 ccattacttt taaatttttt agcattggca atgttgttat aaatcgggtt gaaagtattg
     4201 tctgtgaaac cgaataatga agctacaata attctgttgc cttcatgttt aattttgaaa
     4261 tcggctaatt ctgccttttc tgtagaaatt gtttgagtga taacttttcc atcaacacaa
     4321 gtcgcaatga agtgttcttt accgctgctg aatacacctg aaaaagtacc attgtcattt
     4381 actgttccat caaaaaaagt actgttaggt aatacagttt tccagacgtt gtttaactga
     4441 ttactgaaac aataaatgct gtcattagtg ctaatggtga aataatgttt gttaatggaa
     4501 gtgttatagt tgaaaaaact cttctgatta aattttacaa catcttttcc tgtaattaaa
     4561 actattgttt gcttaaaata tcctctgagg tcaaaaatat cagcgcaaaa ttcaagatta
     4621 tctattcctt ttgagtagaa aacttttatg gttgaatcgc tcagcactac atcatatacg
     4681 cgcaccggag actgtaaaaa tcttattgca gcagtatctt tcaggatgaa tttgtcatca
     4741 tagcgtagca atgttaattt gagttttttc caggccgttg ttaaaaaaat actgtcgctg
     4801 atatcctgaa tcattttaat attacttcct gcagcataaa aatagccgtt gttagtgccc
     4861 aggaattttt gataaggtcg ttcgtttaat ttaactgaag ggttccatat cagattttgc
     4921 ccaaaaacag aaacactaca gaataaaagt aagataagga cattactttt tgttttcata
     4981 tactttaaca ccgtttgagt aagttgcttc tacaggtaat ttaaataaaa cactgtccgg
     5041 tgcagtcatc aggtcgtcat tcataataac aaagtctgct gccttacctt tttccaaaga
     5101 gcctttttca ttctcttcaa acatagaata ggcagcccaa atggtcatgc cgcgtaatgc
     5161 ttctgtgcgg ctgatactgt tttctatttc aaaaccttca ggcggaaatc ccttctggtc
     5221 tttacgcact acagcagcat agaaaccata aagcgggtta atatcttcaa cgggaaagtc
     5281 gctgcccaag gctaacatgc ctgtttcttt taacaaggca ttatttgaat acgcatcatg
     5341 aattcttacg ctgccaagtc ttttttctgc ccaatacata tcactggtag catgtgttgg
     5401 ttgaa
//
LOCUS       DAOQNP010000115         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_6245_length_5345_cov_5.777127, whole genome shotgun sequence.
ACCESSION   DAOQNP010000115 DAOQNP010000000
VERSION     DAOQNP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6245_length_5345_cov_5.777127"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..703)
                     /gene="pdxH"
                     /locus_tag="PKY09_11755"
     CDS             complement(56..703)
                     /gene="pdxH"
                     /locus_tag="PKY09_11755"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010600734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNB13568.1"
                     /translation="MDNIHNVLKNVRTDYDAEAFNAQLSDKNPIVQFEKWFTLASQSN
                     LPDVNAMNLATADKSGKPSSRIVLLRDATADGFTFFTNYNSKKGRQLIENPFAALNFF
                     WPQLAKQIRVEGTVEKVSDAVSDDYFSSRPLESRLGAWTSDQSTVIDSRSILENKFEA
                     LKKSMGDNVSRPPYWGGFILKPAYFEFWQGRPSRLHDRVAYTLSHNQWSKTTLSP"
     gene            906..1469
                     /locus_tag="PKY09_11760"
     CDS             906..1469
                     /locus_tag="PKY09_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNB13569.1"
                     /translation="MEDFNQHAATGSLLISEPLQSDSFFKRSVILLSEHDTEGTLGFI
                     LNKLTDVKINDAVEDFPLFDAPLFFGGPVETDTLFYLHTFGHLLKDSKEIVKGVFLGG
                     DYDELKFLINTKQVSPEQIRFYAGYSGWKPNQLEDELYHNAWMLGKGNAEFTFYPEPE
                     VLWSQVLRSMKNKYSLLANFPDDPTLN"
     gene            complement(1523..2179)
                     /locus_tag="PKY09_11765"
     CDS             complement(1523..2179)
                     /locus_tag="PKY09_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNB13570.1"
                     /translation="MSIFIENIHKAYNGLKVLKGISLEIKTNELVAIIGPSGAGKSTL
                     LHIIGTIDKPDTGKVLLNNTNALALSSKKLAAFRNKHIGFVFQFHHLLPEFTSTENVM
                     IPAMIAGESESGALKRSNALLVQLGLEQRMHHKPSQLSGGEQQRVAVARALINNPDFI
                     LADEPSGNLDSGNAEDLHKLFLKLRNDYKKTIVIVTHNNQLAEMADRTITIRDGMLAQ
                     "
     gene            2236..4311
                     /locus_tag="PKY09_11770"
     CDS             2236..4311
                     /locus_tag="PKY09_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13571.1"
                     /translation="MEVILATPYWYILFCLLAGLGCAWWLYGGNSPFNETPWLRRILF
                     FVRFLSFSIIAFLLLSPFIKSAIREVEKPAIAIAFDNSSSVLLDSTHLSASAINQLAE
                     KLTSTFKSDYEADRYSFGSKVDNEVANNDFSEKSTNISEMFEYLKSLYAGRNLGAVIM
                     LSDGIYNKGSNPVYTAEKLKVPVYTIAVGDTTINRDVLITDVAYNKVAFLGNNFPLEI
                     GVAIRQASGASVTLTIKEDSLTLFSRMIAASGNNYFANIPVILSATKKGIHKYKIHLS
                     SVDGEKNLLNNEAEIYVDVSERKMKILLLAQSPHPDVSAIKSVLDKSSLYETKVLFQE
                     QSDENFSNYNLVILHQLPSSKSATLLQRIKTAEVPYLIIGGAQMNVASFNNLGTGVNI
                     SQPVAKMNEVYAKVSDQFGLFSVSENMQNVLSQLPPLFVPFGNYKVEKEVTPLLTQRI
                     GNIQSSMPLWYFSSSGTMRVGVLLGEGLWRWQLNEYALSGNHDVTDELILKSVQYLTS
                     KKKKEPFMIQYKRSYSEGESIEFDATLYNAAGELINEPEVRLNIYNEEKKEFRYTFNR
                     SNNTYHLNAGILSSGRYTFSAETKTGDKVYKETGEFSVVPVLLENFESTANHQLLNVI
                     SSNTGGIMVTPDKINLLIDAIRKNETVKPVSYYHKKLIELISLKSLLIICLFLIATEW
                     FLRKRFGSY"
     gene            complement(4308..>5345)
                     /gene="miaA"
                     /locus_tag="PKY09_11775"
     CDS             complement(4308..>5345)
                     /gene="miaA"
                     /locus_tag="PKY09_11775"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNB13572.1"
                     /translation="FEAVRTAAEEERRLTSEAMQEIYEQGTQEADSMFRQAADRFAGI
                     VHGYDSVVGFGRHLLNKDNKTQIAVEAAKLLNTEIVSFDSRQIFREMSIGTAVPDTQQ
                     MNAVKHHFIHSHSIAEDMSASRYADEATNTINQLLEKNETVVFTGGTGMYMHAVLYGL
                     DPTPNTDYQLRASLEKECAEKGLEYLIHQLKTLDEKSYHKIDIHNPRRVIRALEIIYS
                     SGKTHSSFFEDKNKTSAFNFTLYVLNPDRQLLYDNINQRVDTMIEKGLIDEAKQLLPY
                     RHLTALHTVGYKELFEYFDRNSTLDQAIEKIKQHTRNYAKRQLTWFRQYDNAIWLAPE
                     EMKKKIEEKNL"
BASE COUNT         1583 a          974 c         1050 g         1738 t
ORIGIN      
        1 ttttgtgaaa ctatgctcga cccaaaattt ttttacaatt tacaacttta atgtttcatg
       61 gcgacaatgt tgtcttactc cattgattat gacttaaagt ataggcaacc ctgtcatgca
      121 ggcggctggg gcgaccttgc cagaattcaa aataagcagg ctttaaaata aatcctcccc
      181 aatagggcgg acggcttaca ttgtcgccca tacttttctt gagtgcttca aatttgtttt
      241 ccaaaatgct gcgcgaatct atcaccgtac tttggtcaga ggtccaggca cctaaacggc
      301 tttctaacgg tcgtgatgag aagtaatcgt cagagacagc atcagaaact ttctctactg
      361 tgccttccac acgaatttgt tttgccaact gcggccaaaa aaagttgagt gctgcaaaag
      421 ggttttcaat caactggcgg cctttcttgc tattgtagtt ggtgaaaaaa gtaaagccat
      481 cagcagtggc atcacgcaaa agcacaattc ttgatgaagg cttgcctgat ttatcggcag
      541 ttgcaagatt catggcattt acatcaggta aatttgattg agaagccaat gtaaaccatt
      601 tctcaaattg tactattgga tttttatctg ataattgcgc attgaatgct tctgcatcat
      661 agtcggtacg tacatttttt aaaacattat gtatgttatc catgattaat gtcatttgaa
      721 ttgcaaattt ataaatgtaa gataaatgta ttattcatcc tttctttaac ataagttgcc
      781 ttgttatcag cctgttgaga ggacagtggc atgtttttag caaactgatt tattaaaaga
      841 gattgttaac aacaagtaac tttttcagac agatacgtaa aatataacaa attaatttgt
      901 gttctatgga agatttcaat caacatgcag ctacagggtc actgttaatt tcagaacctt
      961 tacagtccga ttcatttttt aaacgctcag ttattttatt gagcgaacat gatactgaag
     1021 gaacattagg atttatatta aacaaactga cagacgtaaa aattaacgat gcagtagaag
     1081 attttcctct ctttgatgca cctttgtttt ttggcggtcc tgttgaaaca gacacactct
     1141 tttatctgca tacattcgga catcttctta aagattcaaa agaaattgtt aaaggtgttt
     1201 tccttggtgg cgattatgat gagttgaaat ttttaatcaa tacaaaacaa gtcagccccg
     1261 aacagataag attttatgca ggctactccg gctggaaacc caatcagttg gaggatgagt
     1321 tgtatcacaa tgcatggatg cttggcaagg gtaatgcaga atttacattc tatcccgaac
     1381 cagaagtgtt gtggagtcag gtattgcgca gcatgaaaaa caaatattct ttgctggcaa
     1441 atttccctga cgacccgact ttaaactaag ttagtatagt tttaagaagt cagaattagt
     1501 tgttgaaatc taatgtaaaa atttattggg caagcatacc atcacgaatg gttattgtcc
     1561 tgtcggccat ttctgcaagt tgattattat gcgtcactat gacaatggtt tttttataat
     1621 cgtttcgcag tttcaaaaac aatttatgca agtcttctgc attgcctgaa tctaaatttc
     1681 ccgaaggctc atctgcaaga ataaaatcag gattgttaat taatgccctt gcaacggcaa
     1741 cacgctgttg ttcaccaccc gatagttgcg atggcttgtg atgcatgcgc tgttcaaggc
     1801 caagctgaac caacaacgca ttacttcgct ttaaagcacc tgactcactt tctccggcaa
     1861 tcatagcagg aatcataaca ttttctgtag aagtaaattc aggcaacaga tgatgaaact
     1921 gaaaaacaaa gccaatgtgt ttattcctga aagctgctaa tttttttgaa gaaagtgcta
     1981 aggcatttgt attgttaagc aatacttttc cggtgtctgg cttgtcaatt gtgcctataa
     2041 tatgcagcaa cgtggacttt cctgcaccgg aaggacctat gatagctacc agttcattgg
     2101 ttttaatttc aagcgaaata cctttaagaa ccttaagtcc gttataggct ttatgtatat
     2161 tttcaatgaa tatgctcacg cagctaaaat aagtatcatt ccaatactat tatatttgcc
     2221 cctcatcgtt ttgttttgga agtaatactc gcaacaccat attggtatat tttgttttgt
     2281 ctgcttgccg gcctgggctg tgcatggtgg ctttatggtg gtaattcgcc atttaatgaa
     2341 acaccctggc ttaggcgtat tttattcttt gttagatttt tatcattttc aatcattgca
     2401 tttttgctgt tgtcaccttt tattaaatcc gccatacgtg aagttgaaaa acctgccatt
     2461 gccattgctt ttgataactc ttcttccgta ttacttgatt caacacatct ttcggcatct
     2521 gcaataaatc aactggcaga aaaacttact tcaacattca aaagtgatta tgaagccgat
     2581 cgttattctt ttggtagcaa ggttgataat gaagttgcca ataatgattt ctccgaaaag
     2641 agtacaaata tttcagagat gttcgaatat ttaaagtcgc tttatgccgg acgaaatctt
     2701 ggagccgtaa tcatgttatc tgatggaatt tataataaag gcagcaaccc tgtttataca
     2761 gcagaaaaat taaaagttcc ggtttatacg atagctgttg gcgatacaac catcaatcgt
     2821 gatgtattaa taacagatgt tgcatacaat aaagtggcgt ttcttggtaa taatttccct
     2881 ttagaaatag gtgttgccat taggcaggca tccggagcat ctgttacact aaccattaaa
     2941 gaagattcac tgacgctgtt cagcagaatg attgccgcat caggtaataa ttattttgca
     3001 aacataccag tcatactgtc tgctactaaa aaagggatcc ataaatataa aatacatctg
     3061 tcttctgttg atggagagaa aaatctgctc aataatgaag cagaaattta tgttgatgtt
     3121 tccgaacgca aaatgaaaat attgctgttg gcacaatcgc cacatcctga tgtttcagca
     3181 ataaaatctg tacttgataa aagcagtctg tatgaaacaa aagttctttt tcaggaacag
     3241 tccgatgaaa atttcagcaa ctataatctt gtaattctgc atcagcttcc atcgtcaaag
     3301 tctgccactc ttttacaaag aattaagacg gcagaagtac cttacttgat tattggtgga
     3361 gcacaaatga atgtggcatc attcaacaac ttaggcacgg gtgtaaatat cagtcagcct
     3421 gttgccaaaa tgaatgaagt atatgctaag gtaagcgatc agtttggttt gttcagtgtg
     3481 tctgaaaata tgcagaatgt gttgtctcaa ctgccgccat tatttgtgcc atttggaaat
     3541 tataaagtag agaaagaagt gactccattg ctgacacagc gtattggtaa tatacaatcg
     3601 tcaatgccac tttggtattt ttcttcttca ggaactatgc gagtaggtgt tttacttggt
     3661 gaaggtttgt ggcgctggca attgaatgaa tatgcgctat caggaaatca tgatgttaca
     3721 gatgaattaa ttttaaaatc ggttcagtac ctgacttcga aaaagaaaaa agaacctttc
     3781 atgatacagt acaagcgctc ttattcagag ggagagagta ttgaatttga tgccacactt
     3841 tacaatgcag caggtgaact aatcaatgag cctgaggttc gtttgaatat ttataatgaa
     3901 gagaaaaaag agtttcgata cactttcaac agaagtaata acacctatca tttaaatgca
     3961 gggatattgt catccgggcg ttataccttc agcgctgaaa caaaaacagg tgataaggtt
     4021 tataaggaaa caggagagtt ttctgtagtg cctgtattgc ttgagaattt tgaatcaact
     4081 gccaatcatc aactactcaa tgtcatttca tcaaacaccg gaggtattat ggtgactccc
     4141 gataaaatta atttattgat tgatgctatc cggaaaaacg aaacagtgaa acctgtatct
     4201 tattatcaca agaaactgat tgagttgata agtttgaaaa gtttacttat aatctgcttg
     4261 tttttaatag caacagagtg gtttttgaga aaacgtttcg gttcttatta aaggtttttc
     4321 tcttcgatct tttttttcat ttcttcaggt gcaagccaaa tggcattatc atactgcctg
     4381 aaccatgtta attgtctttt tgcataattg cgtgtatgct gttttatttt ttcaattgcc
     4441 tgatcaagtg tactgtttct gtcgaaatac tcaaacaact ctttatagcc aacagtgtgc
     4501 aatgctgtaa gatgccgata gggtagtagt tgttttgctt catctatcaa tcctttttct
     4561 atcatcgtat ccacacgttg attgatgttg tcgtaaagta attgtctgtc gggatttaag
     4621 acataaagcg tgaaattgaa agctgaagtt ttgtttttat cttcaaaaaa ggacgaatgt
     4681 gttttgcctg agctgtaaat aatttctaat gcacggataa ccctgcgtgg gttgtgaatg
     4741 tctattttat gatagctttt ttcgtcaagg gtttttaatt gatgtatcag atactccaat
     4801 cctttttcag cacattcttt ttccagactt gctctaagtt gataatccgt atttggagta
     4861 gggtcgaggc catagagaac agcgtgcata tacatgccgg ttccaccggt gaaaactact
     4921 gtttcattct tttctaacaa ttgattgatg gtgttagttg cttcatcggc atagcgactt
     4981 gcactcatgt cttctgcaat agaatgtgaa tgaatgaagt gatgtttaac tgcattcatt
     5041 tgttgggtat caggcactgc cgttccgata gacatttcac gaaaaatctg tcgtgaatcg
     5101 aaagaaacaa tttctgtatt aagaagcttg gcagcttcaa cggcaatttg tgttttgttg
     5161 tctttgttaa gcagatggcg gccaaaacca actacgctgt cataaccatg cacgatgccg
     5221 gcaaagcggt cggcggcctg acggaacatg ctgtcggctt cctgcgtgcc ctgctcatag
     5281 atttcctgca tcgcctcgga ggtgaggcgg cgttcttctt cggcggcagt gcgcacggct
     5341 tcgaa
//
LOCUS       DAOQNP010000116         5301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_6327_length_5301_cov_8.004194, whole genome shotgun sequence.
ACCESSION   DAOQNP010000116 DAOQNP010000000
VERSION     DAOQNP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6327_length_5301_cov_8.004194"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="PKY09_11780"
     CDS             <1..1507
                     /locus_tag="PKY09_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13573.1"
                     /translation="KNGIELTGCDTSTVSCNVISGRYPAQTYQNKGIYSRFNNECYFG
                     CNFTDSTRTGVFFSGSNSGTKFRGNEIKRHNIGLVLDSSAVIDTQAHAGNIWTSIYTS
                     GYGAWNDNWQPNSNLFLSLFITNPSLGATYNPSVPLGVPNDLGWFFYDTLGHTFECDG
                     LLLCTDNPAKRSDGSVEIKEAIASDSAVSSAFVPESKMIAQMDLFKELKEDSVLRSSK
                     TVFENFVSDKENNSIGYLYKTNEKLKEVGSYSLQQQSTLHLADSIMYVYLNEVQTLDS
                     IAANDSTVDNLQARELLMNNIQSQIIIKENIINQVQPDNVSKINQAVVFNSLVSANEL
                     PDYNKKVVNEIYFHYLLQGKDTLSAYSSQIFSVATQCPLTGGKAVYVARALMSLLNDT
                     LEYEDNCTQNVNFRLQQNEKVNEIIKNNEIKIIPNPAYTFADVIIPDDYSGICKVKLI
                     DASSRILLEQNFDCSKKKYRIETSTLQSGVYQVQVIINGSINKNSKLIILK"
     gene            1504..2982
                     /locus_tag="PKY09_11785"
     CDS             1504..2982
                     /locus_tag="PKY09_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13574.1"
                     /translation="MKKLFAVLFYLPLLTQGQMNNPFLIGYGNWAVRARMVFDSANYI
                     LTQESRQMGFVGTEATISDKNGNFLMSSNGIWIADSTGNIMMNGDSLNPSQMTASNPN
                     GLLLPNANIILPYPDNATKYVLFHHTATWNGTYNPTYEVFYSVIDMTQNGGLGGVDST
                     QKNVIAFQDTLNWGLAACRHANGRDWWILAIKDSSDIAFKMLFTNNGLASVTSQKLNV
                     PLAWGNVTQPTFSPDGTKFGYTTYVPYQPDSGFICVFDFDRCSGQFSNKRVAFVNTSS
                     LWGFAFSNNSNLAYAASTLRVFQFNLNSLTVDTVAVYDGFYSPYTWCCATSFLDMYLA
                     ANGKIYITSGSSVQHIHEMNYPDSAGIACDLQQHAINLGVWSFRAVPNHPNYYLGCDT
                     TQTTCPCLTTGVNEMEQHDFNFKIFPNPNNGNFNVSYLLPQNANGRLEVIDINGRVIY
                     NQYLPQWSTLQNLNLSLPSGIYALKLSSKNYFTTKRFLVQKQ"
     gene            complement(3087..4550)
                     /locus_tag="PKY09_11790"
     CDS             complement(3087..4550)
                     /locus_tag="PKY09_11790"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNB13575.1"
                     /translation="MQTTATNDVNYEQDLNEWIKAEKAAIELIHVTGSLWFDKSVELV
                     MFRNQMIDRSSSQLLNLIQYSKEIVKKPINIFDALAIAKELNAIDLAPSRLDLGRLAH
                     EWLVDKEKYQTVSKFIADKLKDFIGKGKKTLTPKDVVLYGFGRIGRLLARELTIMAGK
                     GEQLRLRAIVTRDKSDEDIIKRADLLRTDSVHGPFPGTVIEDIQNRALIINGHTVHMI
                     SAKNPEDIDYTKYGINNALLVDNTGVARDREGLGKHLLSKGVDKVLLTAPGKGDIPNV
                     VYGVNQTQHDANEKIYSAASCTTNAIVPVLAVINKTLGIERGHIETIHSYTNDQNLLD
                     NYHKKYRRGRSAALNMVITETGADKAVSKALPQLAGKITGNAVRVPTPDVSLAILNLT
                     LGKNSDKDAINEILKEAALKGDLVEQIQYSYGNELVSTDLVGNPCASIVDSPATIVSK
                     DGKTAVLYVWYDNEYGYSRQVIRFAKYLADVIRLTYY"
     gene            4765..4929
                     /locus_tag="PKY09_11795"
     CDS             4765..4929
                     /locus_tag="PKY09_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13576.1"
                     /translation="MKQLLNIFIMQWADKTISLVGLVLFKSSVLAGLLSFTSKKKKET
                     YKVVIEKEKL"
     gene            complement(4937..>5301)
                     /locus_tag="PKY09_11800"
     CDS             complement(4937..>5301)
                     /locus_tag="PKY09_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13577.1"
                     /translation="RSLVLEDKMINNINTLKSELTPANILNKTFKSLMFENKTGIAGS
                     ALRIGAEYLIGRGAQRKSGSVLYGTLGYVAAKSLINYALDNKKSLLDTLSKLLNKIDL
                     RKNDPDNIFDQSTADDHF"
BASE COUNT         1717 a          926 c          923 g         1735 t
ORIGIN      
        1 caaaaatggt attgaactga caggttgcga cacttcaaca gtgagttgta atgttataag
       61 cggacgttat cctgcacaga cttatcagaa caagggaatt tatagccgat ttaacaacga
      121 atgttatttt ggttgtaatt ttactgacag tacacgaaca ggcgtatttt ttagtggaag
      181 taattcaggt actaaattca gaggcaatga aataaaaaga cataacattg gtttagtatt
      241 ggacagttcg gcagtaatag acacacaggc tcatgcaggt aatatttgga caagtattta
      301 caccagcggc tacggtgctt ggaatgataa ttggcaacct aactcgaatt tgtttttatc
      361 cttatttatt actaacccaa gtttaggtgc tacatataac ccttctgtcc cattaggagt
      421 accaaatgat ttgggttggt ttttctatga tacattagga catacttttg aatgtgatgg
      481 cttacttctt tgtactgaca atcctgctaa acgcagtgat ggctcagtgg aaataaaaga
      541 agccattgct tccgacagtg cagtatcttc tgcctttgtt ccagaatcaa aaatgatagc
      601 gcaaatggat ttgtttaaag agttgaaaga ggacagcgtt ttaagaagtt caaaaactgt
      661 ttttgaaaat tttgtttcag ataaagaaaa caattccatt ggttatttgt ataaaacaaa
      721 tgaaaaattg aaggaagtag gtagttattc attacaacaa caatcaacat tacatttggc
      781 ggatagcatc atgtatgtgt atttgaatga agttcaaaca ttagatagta tagctgcaaa
      841 tgatagtacg gttgacaatc tgcaagcaag ggaattatta atgaacaaca tccaatctca
      901 aataataata aaagagaata taataaatca agttcagcct gacaatgttt caaaaataaa
      961 tcaagctgta gtgtttaatt cacttgtttc ggctaatgaa ttacccgatt ataataaaaa
     1021 ggttgtgaat gaaatttatt ttcactatct gttgcaaggt aaagacacgc tgagtgcata
     1081 ttcttcacaa atcttttcgg ttgctacaca atgcccgctt acaggtggta aagcagtata
     1141 tgtagcaagg gcgttaatgt ctctactgaa tgatacatta gagtatgagg ataattgcac
     1201 gcaaaatgta aatttcagat tacaacaaaa tgaaaaggta aatgagataa ttaaaaataa
     1261 tgaaataaaa ataattccaa accctgctta cacttttgct gatgttatta tccctgatga
     1321 ctattcagga atttgtaaag taaaattaat tgatgcctct tcccgaatac ttttagaaca
     1381 aaactttgat tgttcaaaaa agaaataccg aatagaaaca tcaacgcttc aaagcggtgt
     1441 ttatcaggtt caggttatca ttaatggaag tattaataaa aacagtaaat taattatttt
     1501 gaaatgaaaa agttatttgc ggttttattt tatctgcccc tgctaacaca ggggcagatg
     1561 aataatccgt ttttaatagg atacggtaat tgggctgttc gtgcaagaat ggtttttgac
     1621 tctgcaaatt atatcttaac acaggaaagt cgccaaatgg gatttgtagg aacagaggca
     1681 acaataagtg ataaaaacgg aaatttttta atgagcagta acggaatatg gatagctgac
     1741 tcaacaggaa acatcatgat gaatggggat agccttaatc caagtcaaat gactgcctca
     1801 aaccctaatg gtttattatt acctaatgct aatattattt tgccatatcc tgataatgca
     1861 accaaatatg ttttgtttca tcatacagca acatggaacg gaacatataa tccaacctat
     1921 gaagtttttt acagcgttat tgacatgaca caaaatggcg gattaggtgg agtggattca
     1981 acacaaaaaa atgttattgc atttcaggat acattaaatt gggggctagc agcttgccgc
     2041 catgcaaatg gaagggattg gtggatatta gctattaaag attccagcga tatagctttc
     2101 aaaatgttat ttaccaataa cggattggca agtgttacat ctcaaaaatt aaatgttccg
     2161 ttagcatggg gtaatgtaac tcaaccaact ttttcacctg acggaactaa atttggatac
     2221 actacttatg tgccatatca acccgatagt gggtttattt gcgtttttga ctttgataga
     2281 tgttcaggac agtttagcaa taaaagagtt gctttcgtta atacttcttc tctttggggg
     2341 tttgcatttt ctaataattc taatttagct tatgcggcaa gtaccttacg agtttttcaa
     2401 tttaatttaa actcattgac ggtggatacc gttgcagttt atgacggatt ttattcacca
     2461 tatacatggt gttgtgctac atcattttta gatatgtact tagctgctaa cggaaaaatt
     2521 tatataacta gcggtagcag tgttcaacac atacatgaaa tgaattatcc agatagtgca
     2581 ggcatagctt gcgatttgca gcagcatgcc atcaatttag gtgtgtggag ttttcgtgct
     2641 gtccccaacc accccaacta ctaccttggc tgcgatacca cacaaaccac ctgcccgtgt
     2701 ttaaccacag gtgttaatga aatggagcag catgatttta attttaaaat atttccaaat
     2761 cctaataacg gtaattttaa tgtttcatac ttattgccac aaaatgctaa tggaaggtta
     2821 gaggtaattg atattaacgg gagagtaata tataatcagt atttacctca atggagtacg
     2881 ttgcaaaatc taaatttatc attgccatct ggtatttatg ctttaaaact ttcttcaaag
     2941 aattatttta caacaaagag gtttcttgta caaaaacaat aattcccata tactaacagc
     3001 ataaataaag aagttacata ttcaaacagt ataaaaaagg cttccccgtt ttgagaaagc
     3061 cctttgttta ctatatattt taatgcttag tagtatgtca atctgataac gtctgcaagg
     3121 tattttgcaa atcgaatcac ctgacggctg tagccatatt cattatcgta ccaaacataa
     3181 agtactgctg tttttccatc tttacttaca atggtagcag gactatcaac aatacttgca
     3241 catggattac caacaaggtc tgtacttacc aattcatttc cataagaata ttgaatctgc
     3301 tcaaccaaat cgcctttaag tgctgcttcc ttcagaattt cgttgatagc atctttatct
     3361 gaatttttac ctaaggtcaa attcagaatt gctaatgata catcaggagt aggaacccta
     3421 acagcattac ctgtaatttt acctgccaat tgaggtaaag cttttgatac tgctttatca
     3481 gcacctgttt cagtaattac catatttaaa gcggccgagc gacctcttct gtatttttta
     3541 tgatagttgt caagaagatt ctggtcgttt gtataagagt gaatggtttc aatatgccca
     3601 cgttcgatac ctaatgtttt attgataaca gccaacacag gaacaatggc attggtagta
     3661 caagatgctg cagaataaat tttttcattt gcatcatgtt gtgtttgatt tacaccataa
     3721 acaacattag gaatatctcc tttgccggga gctgttagta atactttatc tactcccttt
     3781 gaaagtaaat gtttacctaa gccttcacgg tcgcgtgcaa caccggtatt atcaaccagc
     3841 agggcattgt taatcccata ctttgtataa tcaatatctt caggattctt tgctgaaatc
     3901 atgtgtacgg tatgtccatt aattatgaga gcacgatttt gtatatcttc aatcacagtg
     3961 cccggaaatg gcccatgaac actatctgta cgtaacaaat ctgcacgttt gataatatct
     4021 tcatcacttt tatcgcgggt cactattgca cgtaaacgaa gctgttcgcc tttgccagcc
     4081 atgatagtta gctcgcgcgc cagcaatcgg cctatacgac caaaaccata aagaacaaca
     4141 tctttaggtg ttaatgtctt tttgccttta ccgataaaat cctttagttt atctgcaata
     4201 aacttactta ccgtttgata tttctcttta tcaacaagcc attcatgagc caaacgtcct
     4261 aaatcgaggc gtgatggtgc aagatcaata gcatttaact ctttggcaat agctaaagcg
     4321 tcaaaaatat taatcggctt ttttacaatt tccttagaat actgaatcag gttcaacaat
     4381 tggctgctgc tgcgatcaat catctgattg cgaaacataa ccaattctac tgatttgtcg
     4441 aaccataaag atccggtaac atgaattaac tcaatggcag ctttctcagc cttaatccat
     4501 tcgtttaaat cctgttcata gtttacatca tttgttgctg ttgtttgcat tttttattga
     4561 ttttattcag gctgcaaata tactccatgt ttacaaacgt tgtacaacaa aattgcccca
     4621 tttattacat tacaactact ttgtaattca gtgttttaaa atgattttct gattaactaa
     4681 acaacaactt gtttaaaaca ttttactgtt ctatccgttt aacggattat tagattgact
     4741 tttgttgtca gaaatttaag gtggatgaaa cagcttttaa acatatttat aatgcagtgg
     4801 gcagataaaa ctatcagtct tgtcggactg gtgttattta aatcttctgt tcttgcaggt
     4861 ctgctaagtt ttacttccaa aaagaaaaaa gaaacttata aggtagtaat tgaaaaggaa
     4921 aagctttaat cgctattcag aagtgatcat ctgctgttga ttgatcaaaa atattatccg
     4981 gatcattttt tctcaagtca attttattca acaacttact tagagtatcc agtaaagact
     5041 ttttgttgtc aagagcatag ttaatcagtg attttgcagc aacatagcct aacgttccat
     5101 acaaaaccga cccactttta cgttgagcgc ctctgccaat taaatactct gcaccaatcc
     5161 ttaatgcact accggcaata ccagtcttgt tttcaaacat taatgattta aaggttttat
     5221 tcagaatatt ggctggtgtc aactcacttt tcaatgtgtt gatattatta atcattttgt
     5281 cttctaaaac caaactgcgt t
//
LOCUS       DAOQNP010000117         5266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_6399_length_5266_cov_8.295529, whole genome shotgun sequence.
ACCESSION   DAOQNP010000117 DAOQNP010000000
VERSION     DAOQNP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6399_length_5266_cov_8.295529"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2866)
                     /locus_tag="PKY09_11805"
     CDS             complement(<1..2866)
                     /locus_tag="PKY09_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB13578.1"
                     /translation="MLRNVYLTCLLTLIFGSAAWAQSGSIKGRVLDKTTNEPLPFASV
                     IAVMNGQQMGGAQTDFDGNFTIKPLGAGTYSVKATFVGYTTTEVTGVLVSVDKITFAD
                     VKMGKGAVDITAVEVTAYKVPLIDKGNPAVQTTITQEEIKAIPTRDVRSVAATAAGVF
                     QRDEGDDLNVRGSRDDATDYYIDGVKVRGSSQLPQSAIEQVTVITGGVPAQYGDATGG
                     IISITTRGPSKKFTGGIELATSELTDKFGYNLASGNISGPIYMKKDKDGNKTPVVGFF
                     LGVEGEWIKDPDPSAIGSKRVKGSVLSDLESNPLMPNIVNGTIGGTRARSEFIHRSDI
                     ETMKYKPNTRSGEYKVSGNIDFQPANNINIKVGGSYDYHDYREYTYNYSLFNYKNYSR
                     HITNDYRGYLRFTQRFNTEGKEGSKSILKNVYYSLQFDYSKAFELVQSEQHKDRLFDY
                     GYWGRFNTLRRPVFAGNELLGYLPYLTVFTPSGNNPNTEAYTNQYLSFFDENYAAHAN
                     EYYETGNTSQFTNTAFYSNPFVIRANGGLINGQRAGTVYSMWYNTGRTIDGYNYIDRD
                     QYRASAMFSADIKNHAISLGFEYEQRVDRQFNIGPTGLWILMNNYANFYLNEANPEAG
                     SYTINDTIYHPNTYTNTSGQVGFYENVRKQLGLSNTEYVDVFNYDPSNFSLSMFSPDE
                     LGTAGGILNNSYVYYGYDYKGNRQKTLSPSATFDNFFKKDANGVYQRNIAAFQPIYLA
                     GYIQDNFSINDLIFNVGLRVDRFDANQRVMKDPYLLYQAYTAGELNFQRPDNIGSDYV
                     VYVSDFNVDKNTLTANDILGYRSGDQWYDKNGVALTDPRILERQSSSARVLPLLVNKD
                     DDIKSGNYEASKTFKDYEPQLTFMPRVAFQFDINDQAQFFANYSILTQRPAGRLRNDP
                     WAYYNFEQGSTISNPNLKPEKSVNYELGFRQILSRSSVITI"
     gene            complement(3014..3451)
                     /locus_tag="PKY09_11810"
     CDS             complement(3014..3451)
                     /locus_tag="PKY09_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13579.1"
                     /translation="MEFKIIIYIIIGIAWYVINNYKKIAEENRKRVFGKPVIYPPTEQ
                     PQPNSPAPVKEPIVVKTIKEKPVIKKRSLPQNTLINKTRKPITVPEIYNVDSTVLTAN
                     SIQVPVNEDLEKKYFNDVIQNRELLKKAIVFGDLINKPLWAKY"
     gene            3593..>5266
                     /locus_tag="PKY09_11815"
     CDS             3593..>5266
                     /locus_tag="PKY09_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNB13580.1"
                     /translation="MRLSSLEIKGFKSFGDRVIIHFDKGVTSVVGPNGSGKSNVVDAM
                     RWVLGEQKMRMLRSEKMENIIFNGTKTRKPANLAEVSLTFVNDKGILPVEFTTVCVTR
                     RLYRSGDSEYLLNGVTCRLKDITDLFLNTGIGSDTYSIIELKMVDDIINDRHNTIKML
                     LEEAAGISKYKIRKKQTLQKLEETDADLNRVNDLLFEIEKSLKQLEYQAKKTEKYYRL
                     KEEYKVVSTALAWFLMRDFTSTIKELTEKEQKQSEEKNTLGRQLENLEQQLSEIKIVS
                     EERQQALGDVQKLLNEKLFLINKYENEHKGRIEKLNYLNEKKLQLEKQSSSEQAQAID
                     IEKQIEQAEKEKSIEEVKTKELENEIQSLKSELETSRKNYDQSVDELRQLNLHILQVR
                     QSVNDHETFIAVRQTRINSLNDQMKRLGEQIEKDKTSLQNLEGELSGHAVKKDQAEKE
                     SHDKKQLNLQLDSEIAQHENAVKELREKLADERRSYEVKNNEYQLTRNLAEKMEGFPE
                     SIKYLKKNSDAFKDIPLLSDIINCDEQFKVAIENYLEPFLNHFIVQSSEQ"
BASE COUNT         1784 a          947 c          923 g         1612 t
ORIGIN      
        1 aaattgtaat tacagaacta cggctcaata tctgacggaa acccaattca taattgacac
       61 ttttttcagg tttaagatta ggattactta tagtacttcc ctgttcaaaa ttataatatg
      121 cccaaggatc atttcttaaa cgaccggcag gacgttgtgt taagattgaa tagttagcaa
      181 aaaactgcgc ctgatcgtta atatcaaact gaaaggcaac acgtggcata aaggttaatt
      241 gaggctcata gtctttgaaa gtcttgcttg cctcatagtt accactctta atatcatcat
      301 ctttattcac caacaaaggt aatacgcggg cagaagaact ctgtctttca agaatccttg
      361 gatcagttaa tgcaacacca ttcttatcat accactgatc gccacttctg taacctaata
      421 tatcgtttgc tgtcaaagta tttttatcta cattaaaatc tgatacatag actacataat
      481 cagagccgat gttgtctgga cgctgaaaat ttaattcgcc agctgtatag gcttgatata
      541 ataaataagg atctttcata acgcgctgat tggcatcaaa acgatctaca cgcaatccaa
      601 cattaaagat aagatcatta atagagaagt tatcctgaat ataaccagcc agataaatcg
      661 gctggaaagc agcaatatta cgctgataaa ctccattggc atccttctta aagaagttat
      721 cgaatgttgc tgatgggctt agtgttttct gacgatttcc tttgtaatca taaccatagt
      781 aaacatagga attattaagt atccctccag cagtacctaa ttcatcagga gaaaacatgg
      841 ataaactaaa attagaaggg tcatagttaa acacatcaac atattctgta tttgacaagc
      901 ccaattgctt acgaacattt tcataaaaac caacttgtcc tgaagtgttg gtgtaggtgt
      961 ttgggtgata gattgtatca ttgattgtat aacttcctgc ttcagggttt gcttcgttca
     1021 aatagaaatt ggcatagttg ttcatcaaaa tccataaccc ggtaggtcca atattaaatt
     1081 gacggtcaac acgctgctcg tattcaaaac ctaacgaaat tgcgtgattc ttgatgtctg
     1141 cagagaacat tgctgaagcc ctgtattgat ctctgtcaat ataattataa ccatctatag
     1201 tacgacctgt attataccac atactataaa cagtaccggc acgctgtccg ttaattaaac
     1261 caccgttagc tctaattaca aagggattac tgtaaaatgc tgtattggtg aattgacttg
     1321 tgttacctgt ttcataatat tcattggcat gtgctgcata attttcatca aaaaacgaaa
     1381 gatactgatt ggtgtaggct tctgtatttg ggttgttacc tgatggtgta aatacagtca
     1441 gataaggcaa ataccctaac aattcattac ctgcaaatac gggtctgcgt aaggtattaa
     1501 aacgacccca atagccataa tcaaataagc ggtctttatg ctgctcagat tgtactaatt
     1561 caaaagcttt actataatca aactgcaaac tataatatac atttttcaga attgactttg
     1621 agccttcttt tccttcagtg ttaaagcgtt gtgtaaaacg caaataacca cggtaatcat
     1681 tagttatatg tcggctgtaa ttcttatagt tgaataaaga gtagttataa gtatattcac
     1741 ggtaatcatg gtagtcataa gaaccaccaa ctttaatatt tatattattt gccggttgaa
     1801 agtcaatatt tccactcact ttgtattctc ctgaacgcgt atttggcttg tatttcatgg
     1861 tttcaatatc agacctatgt ataaactcag aacgtgccct ggttccacca atagtaccat
     1921 taactatgtt tggcattaaa ggattacttt ctaaatctga cagaacactt cctttaacac
     1981 gcttgcttcc gatggctgat ggatcaggat ctttaatcca ctctccttca accccaagga
     2041 aaaaacctac aacaggggtc ttatttccat ctttatcttt tttcatataa ataggtccgg
     2101 aaatattacc gcttgccaga ttatatccga atttatcagt caactccgat gtagctaatt
     2161 caattcctcc tgtaaatttc tttgaagggc cacgtgttgt aatactgatg ataccacctg
     2221 tagcatctcc atactgtgca ggaacaccac ctgtaattac agtaacctgc tcaatagcag
     2281 attgcggcaa ttgtgagctt ccacgaactt taaccccatc aatataataa tctgttgcat
     2341 catcacgtga acctctaacg tttaaatcat caccttcatc gcgttgaaaa actccggcag
     2401 cggttgctgc aacagaacgc acatcacggg ttgggatggc ttttatttct tcctgagtga
     2461 tggttgtttg tactgctgga ttacctttat caattaatgg aactttatag gctgtaactt
     2521 ccactgcagt gatatctact gcacctttgc ccattttaac atcagcgaaa gtgattttat
     2581 ctaccgacac taaaactccg gttacctctg tagtagtata gccgacaaat gtggctttca
     2641 ccgaataggt acccgcaccc aaaggtttaa tggtaaaatt tccgtcaaaa tcagtttgcg
     2701 caccacccat ctgttgccca ttcattacag caattacact tgcaaatggt aatggttcgt
     2761 tggtggtttt gtctaataca cgtcctttaa tggatcctga ttgcgcccat gcagcactac
     2821 cgaaaatcaa ggttagtaag catgttaagt aaacgtttct aagcataggt tgacctattt
     2881 aaatttctga ttattgtatt ataaatgtga agattttgtt aaaaataata gtgcgtaaaa
     2941 gtatgaataa aaaaatttaa cttccaaatt gaatttcaaa aattgacatt attatggcgc
     3001 acccatttta ttttcaatac tttgcccata atggtttgtt gattaaatcg ccaaaaacaa
     3061 tcgccttttt taataattca cggttttgaa taacatcatt gaaatacttt ttctccaaat
     3121 cttcatttac aggaacttga attgaatttg ctgtcaaaac agtgctatcc acattgtata
     3181 tttcaggcac tgtgataggt tttctggttt tatttattaa agtattttga ggaaggctgc
     3241 gtttcttaat aacaggtttt tccttaattg ttttaacaac gataggttcc tttacaggtg
     3301 cgggagaatt tggttgtggt tgctctgtgg gcggataaat aacaggtttg ccaaaaaccc
     3361 tcttcctgtt ttcttctgca atttttttgt agttattaat aacataccag gctatgccaa
     3421 taatgatata aatgataatc ttaaattcca tgaggccaaa tatattttta aattttgaat
     3481 tgtatacatt ttgaattgcc aacaacccgt taataagttt taataggtca acgaatgctt
     3541 ttttttaact tcgcaaactt atttgagaac caattaaaat cattcacagt ttatgcgtct
     3601 ttccagtctg gaaataaagg gctttaaaag ctttggagac agagtaatca ttcacttcga
     3661 taagggtgtt acttctgttg taggaccgaa tgggtcagga aaatcaaatg ttgtagatgc
     3721 catgcgatgg gttttgggcg aacaaaaaat gagaatgttg cgttccgaaa agatggaaaa
     3781 catcattttc aatggtacaa aaacacgtaa gccggcaaac cttgcagaag ttagtcttac
     3841 cttcgtaaat gataaaggaa tattacctgt tgaatttaca accgtatgtg taacaagaag
     3901 actctatcgt tccggtgaca gtgaatattt attgaatgga gtaacatgca ggcttaaaga
     3961 cattaccgac ttatttctga atacaggcat aggcagcgac acctactcca tcatagaatt
     4021 aaaaatggtt gatgacatta ttaacgatcg tcataatacc attaaaatgt tactggaaga
     4081 agctgcagga atttccaaat acaagattcg caaaaaacaa acccttcaga aattagaaga
     4141 aaccgatgct gacctgaacc gtgttaacga tttactattt gaaattgaaa agagtcttaa
     4201 acaattagaa tatcaggcta agaagactga aaaatattat cgtttaaaag aagaatataa
     4261 agttgtatca acagcgttag cctggttttt aatgcgtgat tttacttcca ctattaaaga
     4321 gcttactgaa aaagagcaaa agcaatcaga agaaaaaaac acccttggcc gacaattaga
     4381 aaacctagag caacaactca gcgagattaa aattgtcagc gaagaacgtc aacaagcttt
     4441 aggtgatgta caaaaattac ttaatgaaaa actttttctg attaataaat atgagaacga
     4501 gcataaaggg cgaattgaaa aattaaatta cctgaatgaa aagaagctgc aattagaaaa
     4561 gcaaagttcc agcgaacaag cacaagccat tgatatagag aaacagatag aacaagcaga
     4621 aaaagaaaaa tcaatcgaag aagttaagac taaggaatta gaaaatgaaa tacaatcact
     4681 aaaaagcgaa ttagaaacat cacgcaaaaa ctatgaccag tctgttgatg aactacgaca
     4741 actaaatcta catattttgc aggttaggca atctgttaat gatcatgaga catttattgc
     4801 tgtaagacaa acccgtatca acagccttaa cgatcagatg aagcgattgg gcgagcagat
     4861 tgaaaaagac aagacatcac ttcaaaatct tgaaggtgaa ttatcaggtc atgctgttaa
     4921 aaaagatcag gcggaaaaag aatcacatga taagaagcaa ttaaatcttc aacttgattc
     4981 tgaaatagca cagcatgaaa atgccgttaa agaattgcgc gaaaaacttg ctgatgaacg
     5041 aagaagttat gaagtaaaaa ataatgaata tcaactgacg agaaatcttg cagaaaaaat
     5101 ggaaggtttt cctgagtcaa taaagtattt aaagaaaaac agtgatgctt ttaaagatat
     5161 tccattatta agtgatatta tcaattgtga tgagcaattt aaggttgcaa tagaaaatta
     5221 tttagagccg tttctgaatc attttattgt tcagtcaagc gagcag
//
LOCUS       DAOQNP010000118         5233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_6466_length_5233_cov_8.622827, whole genome shotgun sequence.
ACCESSION   DAOQNP010000118 DAOQNP010000000
VERSION     DAOQNP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6466_length_5233_cov_8.622827"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PKY09_11820"
     CDS             complement(<1..1176)
                     /locus_tag="PKY09_11820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018675843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNB13581.1"
                     /translation="MKEEIKQDLIGREYTTLNLGPTHPATHGIFQNVLKMDGEIIIES
                     TPTIGYIHRAFEKLAERRPYGQITTITDRLNYCSSPINNMGWHMTVEKLAGIKLPKRV
                     EYLRVIIMELARIADHIICNSVIGVDSGAMTGFLYVFQFREHIYEIYEEICGARITTN
                     IGRIGGLERDFSQTAWLKIEKLLKELPKGLKEFEDLLIRNRIFMDRTINCGPISAEMA
                     LAYSFTGPNLRAAGIDYDVRVMNPYSSYEEFDFMIPLGTNGDTYDRFMVREQEMKESM
                     SIIKQAMQKLDKLEDKTSYNAEVPEFYLPPKNEVYNEMEALIWHFKIIMAETSVPKGE
                     VYHCVEGANGELGYYLISDGGRTPYRLHIRRPCFIYYQAYSEMIKGAYLSDAILTMSS
                     "
     gene            complement(1190..1687)
                     /locus_tag="PKY09_11825"
     CDS             complement(1190..1687)
                     /locus_tag="PKY09_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNB13582.1"
                     /translation="MFNTIEPEAFFEQLKTQFAEKILLVEKHYDVTVAEVSHDVIKNV
                     ILFLKEQGFQFLTSCNGLHLPAAEKKFGMIYHLHDFKRNLRIRIKTFTQNDPPHFPTL
                     TDIFPAANWMEREAYDFFGFRFDGHPNLHRILNEDSMEGWPLRKEYPLEDQARFDKDD
                     KMFGR"
     gene            complement(1706..2266)
                     /locus_tag="PKY09_11830"
     CDS             complement(1706..2266)
                     /locus_tag="PKY09_11830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNB13583.1"
                     /translation="MSDKNLTNINQKVERLEGYEGPGFLATTLDKAVGLARKNSLWPL
                     PFATSCCGIEFMATMASHYDLARFGSERLSFSPRQADMLMVMGTIAKKMGPVLRQVYE
                     QMAEPKWVLSVGACASSGGIFDTYSVLQGIDRIIPVDVYVPGCPPRPEQILEGVMKIQ
                     ELVHNESLRRRNSPEYAELMASYGIK"
     gene            complement(2277..2648)
                     /gene="ndhC"
                     /locus_tag="PKY09_11835"
     CDS             complement(2277..2648)
                     /gene="ndhC"
                     /locus_tag="PKY09_11835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNB13584.1"
                     /translation="MVLLSTPIDYLPIAIMFIVAMGFVLTTIFVTHLLGPKRKSEIKL
                     DTFECGIESKENARLPFSIKYFLIAILFVLFDIEVIFMYPWAVSFLKLGMVGFLEMLL
                     FLALLLIGFIYIIRKGALKWE"
     gene            complement(2781..>5233)
                     /locus_tag="PKY09_11840"
     CDS             complement(2781..>5233)
                     /locus_tag="PKY09_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13585.1"
                     /translation="GNCLLSGDRGGAWYQVNIIAPGTLNFDIVPNDFNGSTATDYDFA
                     VWKMAGSGTLATCASIANSGTQTQGLVRCNYSALGVTGCYNVGNSPPLYPGFNGAYES
                     GIPVVAGETYYIFVSNYSNSTAGFTLNFTYTTPSPTTVISSSVSNGGTIIWSGTVSSS
                     WTDPNNWGGCAIPSCSGGTGETDALIPALYVNPPIITGTVSCRNLTINPGATLTVAGT
                     GVLEICKDYKNNGNFVAQAGSRVIFDGPATGTYTGDQNIDGNLTGTNAFYHLDIFKAS
                     MADQVLANQDFDVLGNLKVGYNSGTSATNRLTNFLTQPGGSGKYVKVGGNFLVYQDAV
                     GTAAFVTGGGSVLEFTGSANQDYMNRNSLGSVFVNQTVPSTITVMPHGGTAFGHMLLS
                     TTGTLTFASGKIVVNGSINNAGVPTSNKGYVEMQNPLPGTITGMNNNSYISGTGTPPV
                     IYTLKKATTGAIGIYDLPVGTSTKYSNLSFNVKTLLSPSPAFFLVGFDNTVPASNTAF
                     TGSNPDECSVKYHSMGATALNNGLWRILTGSATAGTAGVLDISLTENGYSNGAGGQTV
                     MFNKLGNPATAADWLLNPLPASACISGTSIPGPVSRTNMQWAAIKPASITIPLMLGTA
                     QSFTPLPVEYLALTAKGVQNTIKVEWSTASEKNNKGFEVQRSTDGQSFEIIGWVNGHG
                     TTNLMNMYQYTDINVSKGITYFYRLKQIDFDGIFDISKKVAASISDNNISFNVSPNPY
                     SEQTNVVYQLEQTSEVTLEVFNKLGQKVTTLHKGVQEPGTYQYPFNAKKYGFSAGVYT
                     VKMTINSQIFTRLLFENN"
BASE COUNT         1579 a         1045 c          945 g         1664 t
ORIGIN      
        1 tgaactcatc gttaatattg catcacttag atatgcacct tttatcattt cagaataagc
       61 ctgataataa ataaaacaag gccgtctgat atgtaaacga taaggagtgc gaccgccatc
      121 actgataagg taatatccta attcaccgtt tgcaccttca acacagtgat acacttcgcc
      181 tttaggcaca gatgtttcgg ccatgataat tttaaagtgc catattaatg cttccatttc
      241 attatacact tcatttttag gaggcagata aaattcaggc acttcggcat tataagaagt
      301 tttatcttcc agtttatcta atttttgcat ggcttgttta ataatgctca tgctttcttt
      361 catttcttgt tcgcgaacca taaaacgatc gtaggtgtcg ccattggttc ctaaaggaat
      421 cataaaatca aactcttcat aagatgagta tggattcatg acgcgaacat cataatctat
      481 accggcagca cggagatttg gtcctgtgaa agaataggct aaagccattt cagcagaaat
      541 tggtccgcag ttaattgtcc gatccataaa tattcgattg cgtatcaaga gatcttcaaa
      601 ttcttttaat ccttttggaa gttcttttaa cagcttttca atcttcagcc atgcagtctg
      661 tgaaaaatca cgctctaatc caccaattct gccaatgtta gttgttattc tggctccaca
      721 tatttcttcg taaatttcat agatatgttc acgaaactgg aaaacatata agaacccggt
      781 catggcacca ctgtcaacac caattacaga gttgcagata atgtgatcgg caatacgtgc
      841 aagttccatg atgataacgc gcagatattc tactcgcttt ggaagtttaa tacctgccag
      901 tttttctact gtcatgtgcc atcccatatt gttgataggc gaggagcaat aatttaagcg
      961 atcggtaata gttgtaatct gaccataggg tctgcgttca gctagttttt cgaatgcacg
     1021 atgaatatat ccgatggtag gtgttgattc aataattatt tcaccgtcca ttttaaggac
     1081 attctgaaaa ataccatgag ttgccggatg tgttggccca aggtttagcg ttgtatattc
     1141 tctgccgata agatcttgtt tgatttcttc tttcattttt tcaattaaat tatcttccaa
     1201 acattttatc atctttatca aatcgtgcct ggtcttcaag cggatattct ttacgtagtg
     1261 gccatccctc catactgtct tcgttcaaaa ttctgtgaag gtttggatgc ccatcaaaac
     1321 gaaatccaaa aaaatcataa gcctcacgtt ccatccaatt agctgcagga aaaatatctg
     1381 tcagtgttgg aaaatgtggc ggatcgtttt gtgtaaaagt ttttatccgg atgcggagat
     1441 tacgtttaaa atcgtgcaga tgataaatca tcccaaattt tttttctgca gcaggaagat
     1501 gcaggccatt gcaggaggtt aaaaactgaa aaccttgttc ctttaaaaat agaattacat
     1561 tttttataac atcgtgtgat acttcagcaa cagtaacatc gtaatgtttt tcaactaata
     1621 aaattttttc tgcaaactgc gttttaagtt gctcaaaaaa tgcttcaggt tcaatcgtgt
     1681 taaacatcag tacaattatt attcactatt ttattccgta acttgccatt agctctgcat
     1741 attcaggaga gtttcttctg cgtaatgatt cgttgtgcac caattcctga attttcatca
     1801 caccttctaa aatttgttcg gggcgtggtg ggcagcccgg cacataaaca tcaacaggaa
     1861 taattctgtc tataccctgt aaaacgctgt aggtatcaaa aattccaccg gaacttgcgc
     1921 aagcaccaac agaaagcacc catttgggtt cggccatttg ttcgtaaact tgtcgcaata
     1981 caggtcccat tttttttgca atagttccca ttaccatcag catatctgcc tggcgtggtg
     2041 aaaagctcaa acgctctgaa ccaaatcttg ccagatcata gtgcgatgcc attgtagcca
     2101 taaactcaat gccacaacat gatgttgcaa agggcagtgg ccataatgag ttttttcgtg
     2161 caagacctac tgctttgtct aatgtggtag ctaaaaatcc aggcccctca tagccttcta
     2221 atcgttctac cttttggttt atgttggtta aatttttgtc gctcatagca atttttttat
     2281 tcccatttta atgcgccttt acggattata tagataaatc ctatcagcag taatgcaaga
     2341 aagagtagca tttcaagaaa gcctaccata ccgagtttta agaaactaac tgcccaggga
     2401 tacataaaaa tcacctctat atcgaacaat acaaataaaa tagcaattaa aaaatatttg
     2461 attgagaaag gcagacgtgc attttctttt gattcaatac cacattcaaa ggtgtcgagt
     2521 ttgatttctg attttctttt tggtcccaac aagtgagtaa caaagatggt ggtgagtaca
     2581 aaccccattg ctacaataaa catgatggca attggcagat aatctattgg tgtgcttaaa
     2641 agtaccataa aaatatttat tctttggcaa aattagcctt tatatgatac aatagcaaag
     2701 caaagcattt tgttttgtaa catcataaaa atcaataagg aggctaaaag cctccttatt
     2761 gatttaatta aaaactaaaa ttagttattt tcaaaaagca gccgtgtaaa tatctgactg
     2821 ttaatggtca ttttaactgt ataaacaccg gcagaaaatc catatttttt agcattgaaa
     2881 ggatactgat aagttcctgg ttcctgaaca cctttatgta gtgtggttac tttctgtcca
     2941 agtttattaa aaacttccag tgttacttca gaagtttgtt ctaattgata cacaacattg
     3001 gtttgttcag aataaggatt aggactaaca ttaaaagata tgttgttatc acttattgat
     3061 gcagcaactt ttttactgat atcaaatata ccatcaaaat caatctgttt cagacgatag
     3121 aaataggtaa ttccttttga aacattaata tcggtatatt gatacatatt cattaagttg
     3181 gttgtaccat gaccatttac ccaaccgatt atttcaaatg attgtccgtc agtactacgt
     3241 tgtacttcaa agcctttgtt attcttctcg ctggcagttg accattctac tttaatcgtg
     3301 ttttgtacac ctttagcagt aagcgcaagg tattcaaccg gtagcggtgt aaatgattgt
     3361 gcagtaccta acattaatgg tatagttata gatgcaggct taattgctgc ccattgcata
     3421 tttgttcttg aaacaggtcc gggaatagac gttccgctga tacaagcact tgccggaagt
     3481 ggatttaata accaatcggc agcagttgca ggattaccga gtttgttgaa cattaccgtt
     3541 tgtccgccag caccattact atatccattt tcagttagtg agatatctaa tactcctgca
     3601 gttccagctg tagcactacc ggttaaaatt ctccacaacc cattattcaa ggctgtagct
     3661 cccattgaat ggtatttaac actgcactca tccggattgc tacctgtaaa agcagtattt
     3721 gaagcaggca cagtattgtc aaatccaaca agaaagaagg caggacttgg tgaaagcagt
     3781 gttttaacat taaatgatag gtttgagtat tttgtacttg ttcctaccgg caaatcataa
     3841 ataccaattg cacctgtagt tgccttttta agtgtatata ttacgggagg agttccggtt
     3901 ccggaaatat aactgttgtt attcatacca gtaatagtac ccggcaacgg attttgcatt
     3961 tccacataac ctttgtttga tgtaggtact ccagcattat taatcgaacc attgactact
     4021 atttttcctg atgcaaaagt taatgttcct gtggtactta aaagcatgtg accaaacgct
     4081 gtcccaccat gtggcatcac tgtaatagta gagggaacag tttgatttac aaaaacacta
     4141 ccaagtgaat ttctgttcat atagtcttgg tttgcggagc cggtaaattc caaaacagag
     4201 ccacctccgg taacaaacgc tgctgtacca acggcatcct gatatacaag aaaattacca
     4261 ccaactttta catattttcc actaccacca ggttgtgtta agaaatttgt caaacggttg
     4321 gtagctgatg tgccggaatt atatccaact tttaaattac caagaacatc aaaatcctga
     4381 tttgccaata cctgatcagc catggatgct ttaaaaatat ccagatggta gaaagcatta
     4441 gtaccggtaa ggtttccatc aatattctga tcacctgtat aggttcctgt tgcaggtcca
     4501 tcaaaaatta ctcttgagcc ggcctgagca acaaaatttc cgttgttttt atagtcttta
     4561 catatttcaa gcactcccgt tccggcaact gttaaagtag ctcccggatt gatagttaag
     4621 tttcgacaac ttactgttcc tgtaataata ggaggattta catataaggc tggaatcaat
     4681 gcatcggttt caccggttcc accgctacaa cttggtatag cacagccacc ccagttgttt
     4741 ggatctgtcc aggatgaact tacagttcct gaccatataa tagttccacc attagaaaca
     4801 ctggaagaaa ttaccgttgt aggacttggt gtggtatagg taaagtttaa agtaaatcct
     4861 gcagtactgt tactgtagtt actaacaaaa atataatagg tttcaccggc aacaacggga
     4921 attccgcttt cataagcgcc attaaatcca gggtataatg ggggggaatt acctacattg
     4981 taacagcctg tgacacccaa ggcactgtag ttacacctga ctaaaccctg tgtttgtgtg
     5041 cctgaatttg cgatggatgc acatgtggcg agtgtacctg aacctgccat tttccacact
     5101 gcaaagtcat agtctgttgc tgttgaacca ttaaaatcat ttggtacaat atcgaaattt
     5161 aatgtgccag gagcaataat attaacctga taccaggcac cacctctatc acctgaaagc
     5221 aggcaattac ctc
//
LOCUS       DAOQNP010000119         4977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_7058_length_4977_cov_6.411621, whole genome shotgun sequence.
ACCESSION   DAOQNP010000119 DAOQNP010000000
VERSION     DAOQNP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7058_length_4977_cov_6.411621"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKY09_11845"
     CDS             <1..545
                     /locus_tag="PKY09_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNB13586.1"
                     /translation="VIVRQMMRKVVIEDAGDTRFLERESVNKIEFMEENDWIMDKMVV
                     TEPGDSEYRPGQIITLRKLRDDNSMLKRKDQKEIEARNAIPATSSPLLQGITQASLHT
                     KSWISAASFQETTKVLNEAAINGKVDYLNGLKENVIVGHLIPAGTGLREYERLIVGSQ
                     EEYDLLMASKESDSEQPINM"
     gene            776..1402
                     /locus_tag="PKY09_11850"
     CDS             776..1402
                     /locus_tag="PKY09_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNB13587.1"
                     /translation="MELKKIFENNQKWIDRKLATDAHYFEKLCEGQSPEVLFVGCSDS
                     RVTAEELMGSQPGEVFVHRNVSNMIISIDLNMNAVVDYAMNFLKVKHIVICGHYFCGG
                     VKAAMQAQDLGILNPWLRNIRDVYRIHKSELNNISDENDRYNRLIELNVQEQCINLIK
                     IASVQKAYRQRNLQVHGWVFDMRTGKLIDLKIDLPKELKDIMEIYDLG"
     assembly_gap    1508..1607
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1824..1897)
                     /locus_tag="PKY09_11855"
     tRNA            complement(1824..1897)
                     /locus_tag="PKY09_11855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1861..1863),aa:Arg,seq:acg)
     gene            complement(1949..2920)
                     /gene="floA"
                     /locus_tag="PKY09_11860"
     CDS             complement(1949..2920)
                     /gene="floA"
                     /locus_tag="PKY09_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681040.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNB13588.1"
                     /translation="MASIPLLILVVAGIILFFLFLYFVPVNLWITATFSGVKVSILNL
                     ILMRFRKVPPSVIVNAMITATKAGLNVTIPDLETHYLAGGNVNNVIKALISADKANIP
                     LSFKMAAAIDLAGRDVADAVQLSVNPRVIDTPPVSAVGKNGIQLVVRARVTVRTNINQ
                     LVGGAGEETILARVGEGIVTTIGSAHDHKSVLENPDQISKTVLNKGLDAGTAFEILSI
                     DIADIDVGENIGAKLQMEQATADLKVAQAMAEERRAAAVALEQEMIAKAQEARAKVIE
                     AETQIPMAMAEAFRSGKLGIMDYYKMENIQADTSMRESIGKPPKGNS"
     gene            complement(2939..3403)
                     /locus_tag="PKY09_11865"
     CDS             complement(2939..3403)
                     /locus_tag="PKY09_11865"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNB13589.1"
                     /translation="MGTIFTIILLILVGIVLLILEILILPGLIAGIIGALMILGGIWY
                     SFHELGNTAGTIVALSTLTVTLATIYLAFRYNVWRRFSLQQSSDSKIERVDEMDIKIG
                     DQGKALSVLRPMGTAVFNNKKIEVQSLGEMIEANATIEVIEVLTNKLIVKKI"
     gene            complement(3501..4304)
                     /locus_tag="PKY09_11870"
     CDS             complement(3501..4304)
                     /locus_tag="PKY09_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNB13590.1"
                     /translation="MEFIYSQRWCELVFENRNKNYGAYHLRQNESNRLLHSWLIAMII
                     IATAIFGIAISGGPSVELIQQYSDKTHQPEIIYNFNKVTPPTLPMPITHTAAAPTIKS
                     NGNGIPVVTDRKIQNVEPIKSVPVTTMATLNSTGGNTSIVPVSSGNGTTGAGATVTGA
                     SGFTLMPEVFPEFPGGEEALIRYVQKSIIFPSDLIRREISGTVYVGFIVDKNGHVSNI
                     KILKGIDNAPELSLEAIRVLEKSPVWKPGLQGGKPVAVAYTLPVTFITK"
     gene            complement(4463..>4977)
                     /locus_tag="PKY09_11875"
     CDS             complement(4463..>4977)
                     /locus_tag="PKY09_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13591.1"
                     /translation="NKRGNPDLLNPSIRIIDFGLSVNMAEPIVTQRLPFSLLYSPPEQ
                     LLNLPELINPSTDLFSLGLTLYESVAKYPPFFNENPEMIMHMQLNTAVAEHRNIHPLL
                     LAFIRKASFKKQLRLPPSKLTMDEIRQTVFEGQQDRFTDCNQMRTILQEVVPNISETE
                     KKSFFRKIFN"
BASE COUNT         1510 a          874 c          921 g         1572 t
ORIGIN      
        1 aagttatcgt tcgtcagatg atgcgtaagg tagttattga agatgccgga gatacacgct
       61 tccttgagag agaatccgta aacaaaattg agtttatgga agagaacgat tggattatgg
      121 ataagatggt agtaacagag cccggtgatt cagaatatcg tcccggacaa attattaccc
      181 tgcgtaagct acgtgacgac aactcaatgc ttaaacgtaa agatcaaaaa gagattgaag
      241 cacgtaacgc tattcctgct acatcatctc cattgttaca gggtatcaca caggcatcat
      301 tgcatacaaa gagttggata tctgcagcat cattccagga aacaacaaaa gttcttaatg
      361 aggcagctat caatggaaaa gtagattacc tcaacggctt gaaagaaaac gtaattgtag
      421 gtcacttaat tcctgcaggt acaggtctgc gtgaatacga gcgtttgatt gttggttcac
      481 aagaagaata tgacttgctt atggcttcaa aagagtcaga cagcgagcag ccaatcaata
      541 tgtaagtttt attttcaaca tatttggtca aacaccattc aggaacttgt ctgaatggtg
      601 ttttgtttta tagatcttat cattctttcg caaaccttta gtttatttgt tatttgatta
      661 tatgatcaaa acacttttta tcaaaaggga atattgttca gaattttatt ttcaatatta
      721 accggtcagg atttaaataa gcttaaccct gttctaaatt aattaaattt gtaaaatgga
      781 actaaagaaa atatttgaga ataatcagaa atggattgac aggaaactgg caacagatgc
      841 gcattatttt gaaaaattgt gcgaaggtca aagtcctgag gtcttgtttg ttggttgctc
      901 cgacagcagg gtaacagcag aagagttaat gggatcacaa cctggtgaag tgtttgtcca
      961 tcgcaatgtg tcaaatatga ttatcagcat tgatctaaac atgaatgctg ttgttgatta
     1021 cgcaatgaat tttttaaagg tgaaacacat cgtaatctgt ggtcattatt tttgtggagg
     1081 tgtaaaagct gcaatgcagg cacaagactt gggcatactc aatccatggc taagaaatat
     1141 tcgggatgtg tatcgaattc ataaaagtga attaaataat atatcggatg aaaacgatcg
     1201 ttacaatcgt ttaatcgagt taaatgttca ggagcaatgt attaatctga ttaaaattgc
     1261 atcagtgcaa aaggcatata ggcaacgtaa tttacaagtt catggctggg tgttcgatat
     1321 gcgaacagga aagttaattg acttaaaaat agatttacca aaagaattga aagacataat
     1381 ggagatatat gatttagggt aggcagatgc cttcaaaatc tcaaagattc accgtaaact
     1441 ttcctgacat ttaaccttgc taattttctt cgctttagtt caatcaaaag ccatgattaa
     1501 taaacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa agcgaaccat
     1621 tgctgattcg ctttttatta agtggtcccg gagggattca aatggcgagg ctttctcgcc
     1681 attgagagtg acagagtcac ctctcaaccc atcaacccat cttgataaat tggattgctt
     1741 tattcgtttc tgtagaaaag ggattcaata aataagatta ataaacaaaa aagcgaacca
     1801 ttgctgattc gctttttatt aagtggtccc ggagggattc aaacccccaa ccttctgatc
     1861 cgtagtcaga tgctctattc agttaagcta cggaaccatt tttttagtgg cgcaaagata
     1921 accaaattag catatttcaa aagagaaatt atgaatttcc ttttggtggc ttaccgattg
     1981 actcacgcat tgatgtgtca gcctgtatgt tttccatttt gtagtagtcc ataatgccta
     2041 acttaccact gcgaaaagct tctgccatcg ccatgggaat ctgtgtctcg gcttcgataa
     2101 ctttcgctct ggcttcttgt gctttggcaa tcatttcctg ctctaaagct acagcagcag
     2161 ccctacgttc ttctgccatt gcctgagcca cctttaaatc tgctgttgcc tgttccatct
     2221 gaagtttagc accgatgttt tcgcctacat caatatctgc aatatctatt gataatattt
     2281 caaatgctgt acctgcgtca agacctttgt ttaatacagt ttttgaaatc tgatctggat
     2341 tttctaaaac acttttgtgg tcatgtgccg aaccaattgt tgtaacgatg ccttcaccaa
     2401 cacgtgcaag aatagtctcc tctcctgcgc caccaacaag ttggttgatg ttggtacgaa
     2461 ctgtaacacg tgcgcgtact accaattgaa ttccattttt acccacagca gaaacaggag
     2521 gggtatcaat aactcttggg tttacagaaa gctgaacagc atctgcaaca tcacgaccgg
     2581 caagatctat tgctgccgcc attttaaatg ataatggtat gttggcttta tcggcagaaa
     2641 tgagtgcttt tataacatta tttacattac cacctgccag atagtgtgtt tctaaatccg
     2701 gaattgtaac atttagtcct gctttagttg cagtaatcat agcatttaca attacactgg
     2761 gtgggacttt tctgaaacgc atcaagatta aattcagaat actgactttc acaccggaga
     2821 aagtagcagt tatccagagg ttaacaggaa caaaataaag aaatagaaaa aacagaatga
     2881 tgcctgcaac aaccagaatg agtaatggaa tacttgccat tttttttgat ttttaagttt
     2941 atattttttt tacaataagt ttatttgtca atacttcaat cacttcaatt gtagcattcg
     3001 cctctatcat ttcaccaagt gattgcactt ctattttttt attattaaat actgctgtgc
     3061 ccattggtct taatacagaa agtgctttac cctgatctcc aatctttata tccatttcat
     3121 caacacgttc aatttttgaa tcgcttgatt gttgaagact gaatctgcgc cacacattgt
     3181 aacgaaaagc aaggtaaatt gtagctaatg ttacagtaag cgtgctcagt gctacgattg
     3241 tacctgcagt atttcccagt tcatgaaaag aataccagat gccacccaaa atcattaatg
     3301 caccaattat tcctgcaatt aatcccggta gaatgagtat ctccagaatc aacagaacta
     3361 tgcccacaag tatcagtaaa atgattgtaa atatggttcc cattttcggt gacaaacata
     3421 ataaaaaggc tgttgcgaaa aagcaacagc ctttattaaa aaacacgaaa ttatttatta
     3481 gatttttgtt gaatcggatt ttatttggta ataaatgtta ccggtagtgt ataagcaact
     3541 gccacaggtt ttcctccctg cagtcccggt ttccaaaccg gagatttttc aagtactctt
     3601 attgcttcaa gtgacaactc aggtgcatta tcaatacctt taagaatttt gatattgcta
     3661 acatgaccat ttttatcaac tataaatcca acataaacag ttccactgat ttcgcgtctg
     3721 attaaatcag atggaaaaat aatacttttt tgaacgtaac gaattagggc ttcttcacct
     3781 cccggaaatt caggaaatac ttctggcatt aatgtgaatc ctgaagctcc ggtgacagtt
     3841 gctcctgcac ctgtagtgcc attgcctgaa gagactggaa caattgaagt gttgccacca
     3901 gttgaattta gagttgccat ggtagtaacg ggtacagatt ttattggttc aacattttga
     3961 atttttctat ctgtcactac gggaatgccg ttaccgttag atttgattgt tggtgcagca
     4021 gcagtatgag ttataggcat tggtagcgta ggcggtgtta ctttattgaa gttatagata
     4081 atttcaggtt gatgtgtctt atctgaatat tgctgaataa gttcaacaga tggacctcct
     4141 gatatggcaa ttccaaatat tgctgttgca ataattatca tggcaatcaa ccatgaatgt
     4201 agaagacgat ttgattcatt ttgtcttaaa tggtatgcac cataattttt gttgcggttt
     4261 tcgaaaacca gttcgcacca ccgctgtgaa taaatgaact ccataatgtt gcttttactt
     4321 tatagatgta tgacgcaaca acattccata ttatttttaa ttgaaaatta ttttacattt
     4381 taatgaccgt aaaaccttat tgtttcttcg agctaaaaat gtaagtattc taatgtttat
     4441 ttctctgtta agaatgaaaa aatcaattaa aaatctttct gaaaaaactc tttttttcgg
     4501 tttcagaaat atttggtact acttcctgta agattgttct catttgattg caatctgtaa
     4561 atctgtcttg ctgtccttcg aaaactgttt gtctgatttc gtccatagtt aattttgatg
     4621 gaggaagtcg gagttgtttt ttaaatgatg ctttacgtat aaaagctaaa agcagaggat
     4681 gtatgtttct gtgctcggct acggctgtat ttaactgcat gtgcattatc atctccgggt
     4741 tttcattaaa aaacggagga tacttagcca cagattcata taatgtaagc cccaggctga
     4801 acaaatctgt tgatggatta ataagttccg gcaagtttaa taactgttca ggtggagaat
     4861 aaagaagcga gaaaggaagt ctttgtgtta ctattggctc tgccatgttg accgaaagtc
     4921 caaaatctat gattcgaata ctagggttta ataaatcagg attgcctctt ttattta
//
LOCUS       DAOQNP010000120         4911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_7233_length_4911_cov_6.658979, whole genome shotgun sequence.
ACCESSION   DAOQNP010000120 DAOQNP010000000
VERSION     DAOQNP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7233_length_4911_cov_6.658979"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(542..1960)
                     /locus_tag="PKY09_11880"
     CDS             complement(542..1960)
                     /locus_tag="PKY09_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13592.1"
                     /translation="MRKYFTLLLVVLYSNTFAQKIKVDAQMIQMGWTFVSPLILKKIN
                     DPLTKEIISKTVPKLINQDAKGAAYEVSDAIYRIKNVKVLNKEFLSQVEKSITDGVKA
                     INRKDYASAVNGLVKTVAFSEAYIKKGILDQPTPPTQVTNASINAVKTDIVTAKEINE
                     KLHLGKNNDYLFFIPTGNFTQKETTEKENLFAEDFNVNIDSEKKFNMGVGRQISSDLQ
                     NVSVERFQTDKNASDKLKETMADILSKSLGEGQIIKSEIATYPNFKSLKYTYLYTDKE
                     TSTSSMAYVLITFQDTSMFIIYFNTPANDFLETSKSFEDLMRTFFIIGIDEISNANSK
                     TYSKAVITKIAINKIPSIKSTGEPWDNPFGNNLPDIYYIITDKNGQILRSLNPMERKE
                     DVNPQNLPVYFSLTGKGFVLDDLKETIFIFLYDYDSVTDPDKVGSVGFKLSDYMFGKD
                     AYKEQITKANGNTEISISVKWE"
     gene            complement(2678..3412)
                     /locus_tag="PKY09_11885"
     CDS             complement(2678..3412)
                     /locus_tag="PKY09_11885"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNB13593.1"
                     /translation="MENKIYLTAVLLTATILFNGCSKKDDDNNNPTPTPVPVASFTYS
                     GAGVQAPATVTFTNSSTNATSYSWDFGDNGTSTDKDPQHTYTSGGVYTVQLTVTGAGG
                     TNSISKTVNIQNTPTTCKITSVKLNAMPFANSSGVSWDTFDGPDVFFKIETQTGTVLF
                     DATSSRFSDIASSSLPVQWNLSTPFSITPLNTNRYIMIYDYDTLDPDDYIGGVSFNPS
                     VSVSGYPSTTTLTYQGVSITLTLQWQ"
     gene            complement(3717..4181)
                     /gene="coaD"
                     /locus_tag="PKY09_11890"
     CDS             complement(3717..4181)
                     /gene="coaD"
                     /locus_tag="PKY09_11890"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNB13594.1"
                     /translation="MKTAVFPGSFDPIHNGHLEIVQRMLPLFDKIIIAIGINPDKKYL
                     FSLEQRLNWLEQVFNNEPKISITSYHGLTAEFCKQQQAQYMIRGLRNSNDFEFEKAIA
                     QMNKKLGGIETVFVISSPVHAPLSSTILRDIIINGGSVADLVPVKIAIKTNN"
     gene            complement(4190..4729)
                     /gene="rsmD"
                     /locus_tag="PKY09_11895"
     CDS             complement(4190..4729)
                     /gene="rsmD"
                     /locus_tag="PKY09_11895"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNB13595.1"
                     /translation="MRIISGRLRGKKITAPDSLPVRPTTDFAKTGLFNILSNHLSFST
                     IAALDLYCGTGNISYELISRGAKSVTAVDSDKRCVAFVQKTFNDIHAENAKAIQSDAL
                     DFLEQCSRPFDLIFADPPFNLNPVQEIAEIVYRKKLLTAQGLLVIEHSSEMQALKSDN
                     LLETRTYGAVAFSIFVAEK"
     gene            complement(4720..>4911)
                     /locus_tag="PKY09_11900"
     CDS             complement(4720..>4911)
                     /locus_tag="PKY09_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 domain-containing protein"
                     /protein_id="HNB13596.1"
                     /translation="NPESQVLNIAGTTEKNSALIVLASKYIRHLQFVQRPSFAFYSYG
                     FDEVAAHYHFSLYNQFLCV"
BASE COUNT         1489 a          838 c          905 g         1679 t
ORIGIN      
        1 aatatgtcga agacatgctt gcagaagaaa ttatcaaagc agaattgcag gaaggggata
       61 atatagaagt ggtttttgat aaagaagcac atctgctcaa agccaatatt accaagtcat
      121 caccgccacc accaaaatcg aggaagaaga aagaagaaaa taattcttaa aaacaaagag
      181 ccactgattt ttcagcggct ctttgttttt aatatttgtt tgttgcaatt gcagtacaat
      241 ttgttcggaa ctcaatacaa accgacaata aaaaacagct gctttgttta aaggcagcta
      301 ataaaatcgt tttatatccc tttttactct tttaattggg ttgtgcaagg ttgacttgat
      361 tttatacgta tcacccgact gtttccatga aaatctactg tcagagttta acacaactgt
      421 ttgattgaaa aatgtaatag acgaagctat gcagttgcga gttttgtaga caggtgagtt
      481 tccagtgtcg tgttgcaagg cttttaattg tgaaagcaaa catggtggta tttcttgaag
      541 actattccca tttaacacta attgagattt ctgtatttcc attcgcttta gtaatctgtt
      601 ccttgtaggc atcttttccg aacatgtaat cacttaattt aaatccaacg cttccgactt
      661 tatctgggtc agttactgag tcgtaatcat ataggaaaat aaaaatcgtc tcttttaaat
      721 catctaatac aaaacccttt ccagtgagcg aaaaatatac cggcaagttt tgtgggttaa
      781 catcttcttt tctttccatt gggtttaaac tacgaagtat ttgtccgttt ttatctgtaa
      841 tgatgtagta aatatctgga agattatttc cgaagggatt gtcccaaggc tcgcctgtgc
      901 tttttattga aggaatttta ttaattgcaa tttttgtgat tactgctttg gaatatgtct
      961 tagaattagc atttgatatt tcatcaatac caataataaa gaaagtccgc attaaatctt
     1021 caaaggattt tgaagtttcg agaaaatcgt ttgcaggtgt gttgaaataa attataaaca
     1081 tggatgtatc ttgaaaagtt ataagaacat atgccattga ggaagtagaa gtctccttat
     1141 cagtatagag atacgtatat ttaagtgatt taaaatttgg ataagttgct atctctgact
     1201 ttatgatttg tccttcgcct aaacttttag aaaggatgtc agccatagtt tcttttaatt
     1261 tatcgcttgc gtttttatca gtttgaaaac gctcaacact aacattttgc aaatcggatg
     1321 atatttgtct tccaacgccc atattgaatt ttttttctga atcaatgttt acattgaaat
     1381 cctctgcaaa taaattttct ttttctgtag tttctttttg agtaaaattt ccagttggaa
     1441 tgaaaaacag ataatcgttg tttttcccaa gatgtaattt ttcatttatt tcttttgctg
     1501 taacaatgtc tgttttaact gcattgattg aagcattagt aacctgtgtt ggtggagtag
     1561 gttggtctag tattcctttt tttatgtatg cttcggaaaa tgcaactgtt ttaactaatc
     1621 catttacagc agaggcataa tcttttcggt tgattgcttt aactccgtct gtaatacttt
     1681 tttccacctg cgataagaat tctttattta aaacctttac attttttatt ctgtaaatag
     1741 catcagagac ttcataagcc gctccttttg catcttgatt aattaatttt ggcactgttt
     1801 tactaataat ttctttagtt agtgggtcat taattttctt taaaatgagt ggcgaaacaa
     1861 atgtccaacc catttgtatc atttgtgcat ccactttaat cttttgtgca aacgtgttag
     1921 aataaagaac taccaaaagc agtgtaaagt attttctcat tttattttgg ttttaaaaag
     1981 tcaaatgtaa atatcttgtg acaacggtag tgcagcaggg taaagtaaaa tgtgaagtaa
     2041 aagaatggta tgcttgtgtg caagcgttgc ttctcttttt attttaaaga agcgatagcc
     2101 aaaacggttt tggtgacttt ctgagctcgt cattttcgct tagcagtaac gttcaatcgg
     2161 agtggttaac attgccgcta acatcagtct gtgaaaaaaa taatttcaga agcatttaca
     2221 aatataaatt aatttcattt gatttaagga aagaaacagc aactaaaaat gccacttatg
     2281 caaggggtca accgtgaaca aattatattt tcaaatagcc aaaacgagcc ttaaagtcca
     2341 atctcttggt tcaaccatca ctttggcaaa gcaaaagcaa cgaggagacg aaagtttgtt
     2401 ggaggtttac acaacactaa acccatctat ctgtgatccg gctgccacgg cagatggtgt
     2461 ttgaataact tattattttt ctaatggaat taaagatgtc gctttgctat gttgagctac
     2521 cgatagctat tggtagcacc ctaccgggga aagtctggcg gagttactta ctcgattatg
     2581 tgcagtgctg ctgtgtggtc gaaataccat attttgaagt tcgtatgagt gtttgtctta
     2641 aagacacaca ttcataacac gtatagtgaa tattatttta ttgccactgc aaggtaagag
     2701 taatggatac accttgataa gtaagggttg ttgttgaagg gtagcctgaa acagaaacag
     2761 atggattaaa actaacgcca ccaatataat catcagggtc aagtgtatcg taatcgtaaa
     2821 tcattatgta gcgatttgta ttaagaggcg taattgaaaa tggcgttgac aaattccatt
     2881 gtactggcaa actacttgaa gctatatcac taaatctact tgaagttgca tcaaaaagaa
     2941 cggttcccgt ttgcgtttca atcttaaaga atacatcggg tccatcaaat gtgtcccaac
     3001 ttacaccgct actatttgca aaaggcattg cattgagttt tacactcgtt attttacatg
     3061 tagtcggagt gttttgaatg tttacagttt tggagatact gttagttcca ccggctccgg
     3121 tcacagtcaa ctggacagtg taaactccac cggatgtata agtatgctgt gggtctttgt
     3181 cggttgaagt tccattatct ccaaagtccc atgaataact tgtagcattc gttgaagaat
     3241 tagtaaaagt tacagtagca ggagcttgaa cacccgcacc cgagtatgta aacgaggcaa
     3301 caggaacagg agttggtgtc ggattattgt tatcgtcatc cttcttgcta caaccattaa
     3361 ataaaattgt tgcagttagt aacactgccg ttaagtagat tttgttttcc attttatatt
     3421 ggagttttaa attaattcct tccgttaatt acttgcagag acgtttttag ccttgcacat
     3481 aacatttaaa tatcagcata ttttaaccta aaacatgctg aatattttac aaatatagcg
     3541 gtataaagca attttacaaa attaccggat atgttatagt taatcagcct ggctttcttt
     3601 tctttaagat aacactcata aatttgctga aatcaagtct tgattcaact actttctatg
     3661 gtacgattta ctgcaagtaa accgttagtt tattgattta attttatgta aggctattaa
     3721 ttgtttgttt tgattgcaat ttttaccggc acaaggtcgg caacgctacc gccattgata
     3781 ataatatcac gcagtatggt tgatgataat ggagcatgta caggactaga aatcacaaac
     3841 accgtttcta taccgccaag ttttttattc atctgtgcta tggccttttc aaattcaaaa
     3901 tcgttgctgt tgcgcaatcc gcgaatcatg tattgtgcct gctgctgttt gcagaattct
     3961 gccgtcagtc catgatagct ggtaatgctg attttgggtt cgttattaaa tacctgctcc
     4021 agccagttta atcgttgctc taagctgaac aaatattttt tatccggatt tattcctatg
     4081 gcaataatta ttttatcgaa taagggaagc atacgttgta caatctccaa gtgtccgtta
     4141 tgaatagggt caaacgatcc ggggaatact gcagttttca tcattgatat tatttttcag
     4201 ctacgaatat actgaatgca acagcaccat aagttcgcgt ttcaagtaaa ttgtcagatt
     4261 ttagagcctg catttcgctg ctgtgttcta taactaaaag tccttgtgct gtcaataatt
     4321 tttttctata aacaatttct gcaatttcct gtaccggatt caaattaaat ggggggtctg
     4381 caaatattaa atcaaaaggt ctgctacatt gttctaaaaa atccaatgca tcagattgta
     4441 tagcttttgc attctcagca tgtatatcat taaatgtttt ttggacaaaa gcaacgcatc
     4501 gtttgtcaga atcaacggct gttacacttt ttgcaccacg cgatatcagt tcgtaagaaa
     4561 tatttccagt gccacaatac aggtcgagtg cagcgatggt agaaaagctg aggtggttgg
     4621 aaagaatatt aaacaaacct gttttggcaa aatctgttgt tggacgaacc ggtaaagaat
     4681 cgggagctgt aatttttttt ccccgtaacc gcccgctgat tatacgcaca aaaactgatt
     4741 ataaagactg aaatgataat gtgctgctac ttcatcaaat ccatagctgt aaaatgcaaa
     4801 tgaaggtcgc tgcacaaact gtaggtggcg gatatatttt gatgccaaaa ctatcaatgc
     4861 tgaatttttt tctgttgttc ctgcaatatt caaaacctgc gactccggat t
//
LOCUS       DAOQNP010000121         4817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_7478_length_4817_cov_8.299874, whole genome shotgun sequence.
ACCESSION   DAOQNP010000121 DAOQNP010000000
VERSION     DAOQNP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7478_length_4817_cov_8.299874"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKY09_11905"
     CDS             complement(<1..689)
                     /locus_tag="PKY09_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNB13597.1"
                     /translation="MKNRYIDLIQQSYDFPQEGFEVIDNSLHFNGINLMDVIKQYGTP
                     LKITYLPKISSQIQKAKRLFKVAMAKVDYKGSYYYCYCTKSSHFQFVMEEALKNDIHI
                     ETSSAFDIPIIRSLYESEKINKDIYILCNGFKREGYIQGIVDLIKDGFTNVIPILDNV
                     NELQEYEKALPGKKFKIGIRIASEEEPNAEFYTSRLGIRSRDIMDFYKNTIRKNPNVE
                     LKLLHFFINTG"
     gene            770..1717
                     /locus_tag="PKY09_11910"
     CDS             770..1717
                     /locus_tag="PKY09_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNB13598.1"
                     /translation="MSGKIKFIEVKSEIGAGTRGASLGVDAIKIAALDYGSSLFNRIE
                     SVEIKTENQMLFETAGTPYAKRIKGIVAIYDRVAKAVSETLKNKDFPIVLAGDHSTAG
                     GTVAGIKMTYPKSKLGVIWIDAHADIHSPYTTPTGNVHGMPMAANLGEDNLANKINKP
                     DKETVDLWTKLKKIGGMSPKITYKDIVYIAVRDIEPQEAYLLKKHNVKMISAAEVKRI
                     AVEKIVRNALAHLSHCTHIYVSFDVDSMDSSISKGTGTPVRNGITERDAGKLCVRLVQ
                     NEKVCCFEICEVNPTLDKENLMAENTFEILQRVVNQINH"
     gene            1799..2380
                     /locus_tag="PKY09_11915"
     CDS             1799..2380
                     /locus_tag="PKY09_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNB13599.1"
                     /translation="MEKFYTIQGEGYHTGKPAFFIRLAGCDVGCVWCDVKESWEASAS
                     NLISVGDLLKDVEESKTPNVVITGGEPAAYNLTVLTNALSEKEYKVWLETSGAYPIQG
                     NFDWICVSPKKFKPPVDAVLQKANELKVVVFNESDFKWAEENASKVSSVAKLFLQPEY
                     SRFDRVMPGIIDYVKRNPQWAISLQTHKYLNIP"
     gene            2391..2816
                     /gene="aroQ"
                     /locus_tag="PKY09_11920"
     CDS             2391..2816
                     /gene="aroQ"
                     /locus_tag="PKY09_11920"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNB13600.1"
                     /translation="MAKRIVIINGPNLNLLGKREPEIYGHQTFDDFAMMLKKEFPEHT
                     IEIFQSNVEGEIINLLHNQGFKADGIIINAGGYTHTSVAIADAIAAIPSPVVEVHISN
                     IHSREPYRHNSLLAANCKGVITGFGLNSYRLALIALINS"
     gene            2836..3171
                     /locus_tag="PKY09_11925"
     CDS             2836..3171
                     /locus_tag="PKY09_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13601.1"
                     /translation="MEQLSNQNFHQRDLIEQTVVQLNKDLLSCGYTVAWNGNTFSAYQ
                     DIVMQLEVIIRQLAKSGSSTLNAWLYRVDIPEKALKKVLSKQDNQQALAQAILERTFI
                     KIMFRNSYR"
     gene            3572..>4817
                     /locus_tag="PKY09_11930"
     CDS             3572..>4817
                     /locus_tag="PKY09_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13602.1"
                     /translation="MKSVLFTLLSLVFTTTVYAQPAGTRTITINDCNYPILGIETNHA
                     SDKIVLTASQGRIFFVNVSDVEANTEVSPVWKNLSLSGLNNGGKPQFSENDQYILLQE
                     SSALKTAITSRKVKDVKYYILNAADGKVVAEGNNVNSVQFLNNSTQILISSNEGIVVK
                     DFLTNKIVAQLKVEDCEMAAISHDSKLIALSFDPDKAAFKEVESIGKNKSELKAARKN
                     KRLISFYSYPDLKKLYNSKEEIDVVYSIKFSKDDKHTYLLSHNVIAERIGTGALNGLS
                     MAAADNRRQTNIQVLDASNGNLVKEIYHATAEPDADYKFSAYSNYFGYSDNDGFIGWK
                     RKLVLIDNNDHKNKVGQYRYQGRRGSSNIFPIYFSFNKTKPVAYLSNGTKLIEWNYET
                     LKEYTDYIEGQDNDSLQSLAINQ"
BASE COUNT         1629 a          784 c          930 g         1474 t
ORIGIN      
        1 attcctgtat tgataaagaa atggagtaat ttcaactcta cattggggtt tttacggatg
       61 gtatttttgt aaaaatccat gatatcacgc gacctgattc ctaatcgtga tgtgtaaaac
      121 tctgcattag gttcttcttc agaagctata cggataccaa ttttaaattt tttacccggc
      181 aaggcttttt catactcctg caattcgttt acattatcca aaatcggaat aacattggta
      241 aaaccgtctt taatcagatc aacaatgcct tgaatgtagc cttcgcgttt aaatccatta
      301 caaagaatat atatgtcttt atttattttc tctgactcat agagtgacct gataatagga
      361 atatcaaaag ctgaacttgt ctctatgtga atatcgtttt tcaacgcctc ttccatcaca
      421 aactgaaaat gcgaactttt ggtacaatag caataatagt agctgccctt atagtccacc
      481 ttagccatgg caactttaaa aagtcgtttg gctttttgaa tctgcgagct gatttttggg
      541 agataggtta tttttaaagg tgtaccatat tgctttatca catccatcag gtttatacca
      601 ttaaaatgga gtgagttgtc aataacttca aaaccttctt gcggaaagtc gtaactttgt
      661 tgaatcaggt caatataacg gttcttcatt tagcaatgcc aaaggcttat ttttgtgcct
      721 tgcgaatgta aaaataacat gaataaaaaa taataatgtt aaaaaattta tgtcaggtaa
      781 aataaaattc attgaagtaa agtctgaaat tggagcaggt accagaggtg caagccttgg
      841 tgttgatgca ataaaaattg cagccttaga ctatggcagc tcacttttca acaggataga
      901 aagcgtagaa atcaagactg aaaaccagat gcttttcgag acagcaggta ccccttatgc
      961 aaaaagaata aaaggcattg ttgctattta tgatagagtg gctaaagccg tttcagagac
     1021 attaaagaat aaagattttc cgattgtact tgcaggtgac cacagtacag caggcggtac
     1081 cgttgcagga ataaaaatga cttacccaaa gtcgaagctg ggtgtaatct ggattgatgc
     1141 ccatgccgat atacactctc catatactac accaacaggg aatgttcacg gcatgcctat
     1201 ggcagcaaat cttggtgaag ataatcttgc aaacaaaata aacaaacccg ataaagagac
     1261 agttgacctt tggacaaagt tgaagaagat aggaggaatg tcacctaaga ttacctataa
     1321 agatatagtt tatattgccg ttagagatat tgaacctcag gaagcttatc ttttgaagaa
     1381 acacaatgta aaaatgattt ctgctgctga agttaagcgt attgcagtgg agaaaattgt
     1441 gcggaatgca ttggcacatc tctcacattg cacacacatc tatgtttcgt ttgatgtgga
     1501 tagcatggat agctcaatct cgaaaggcac aggtactccg gtacgaaatg gaattactga
     1561 acgtgatgca ggaaaacttt gtgtaaggtt ggtgcaaaat gaaaaagttt gttgtttcga
     1621 aatttgcgaa gtcaatccaa cgcttgataa agaaaatctg atggctgaaa atacttttga
     1681 gattttacag cgcgtagtca atcagattaa tcattgatta ttttctatgc aacctgtcgt
     1741 gactgaaaat tataaaaaga atattcttga cacgcttcct gaagggtatt atcctgtaat
     1801 ggaaaaattt tacaccattc agggtgaagg ataccatacc ggaaaacctg ctttttttat
     1861 cagacttgcc ggctgtgatg ttggttgtgt gtggtgtgat gttaaagaat catgggaggc
     1921 ttcagcgtca aaccttattt ctgtaggtga cctcttgaag gatgtggaag agagtaaaac
     1981 tcctaatgtt gttattacgg gaggagagcc cgcagcatat aatttaactg tgcttacaaa
     2041 tgcattgtcc gaaaaggaat acaaagtgtg gcttgaaaca tccggtgctt atccaattca
     2101 aggaaatttt gactggattt gtgtttcacc taaaaaattt aagcctcctg ttgatgctgt
     2161 tctgcaaaaa gcaaatgaac tgaaggtagt tgtttttaat gagtcggatt ttaaatgggc
     2221 agaagaaaat gcaagtaaag tttcaagtgt tgcaaaactt tttctgcaac cggaatacag
     2281 tagattcgac agagtgatgc caggtattat tgattatgta aagagaaatc cccaatgggc
     2341 aatatcgctg cagacgcata aatatttaaa tattccctga caggcttctt atggcaaaaa
     2401 gaattgtaat tatcaatggt cctaacctga atctgcttgg caaacgtgag cctgagattt
     2461 atgggcatca gacatttgat gattttgcaa tgatgttaaa aaaagaattt cctgaacata
     2521 ctattgaaat atttcagagt aatgtcgaag gtgaaattat taacctgttg cacaaccagg
     2581 gttttaaagc tgatggcata atcatcaatg ccggaggcta tacccatacc agtgttgcca
     2641 tagcagatgc tattgcggca ataccgtcac cggtggttga agtgcacatt tcaaatattc
     2701 attcacgcga accgtaccga cataatagcc tgcttgctgc aaattgcaaa ggtgtaatta
     2761 ccggctttgg attaaattcc taccgtcttg cactgattgc tttgataaac tcctgacaag
     2821 aaaaggagga tggaaatgga acaactttct aatcagaatt ttcatcaacg cgatttaatt
     2881 gaacaaacgg ttgtgcagtt aaacaaagat ttattgtctt gtggatatac tgtagcatgg
     2941 aatggcaata ctttttcagc ttatcaggat attgtaatgc aattagaagt aatcattcgt
     3001 caattggcaa agtcaggatc ttcaacttta aatgcatggc tatatagagt agatatccct
     3061 gaaaaggcat taaagaaagt tttgagtaaa caggataatc aacaagcact tgcacaagcc
     3121 atacttgaac ggacgtttat aaaaattatg ttcaggaata gctatcggta agtcataatg
     3181 tataaaagtt ataagatttg aatgtttgtt taattagatt atttaatgat ttgccgattt
     3241 taatatttga tgaatagatt agaaggttga aaagttggaa ccttgccggt taacttcaaa
     3301 aaatattttt agtgcctaaa gaacgacctt atagcagaat atctaaaaac aaaatttttt
     3361 gagtgccata gctatgcgaa agttttttct ttaagccctt ttcgtactgt attctttaag
     3421 gtctttgcgg gatttatttc tacaagtttg attcaatacg ctttcataag agatttgaaa
     3481 catatatttc gatttatcgt attaatagct cacaaaatga tgtgtcaact tcttacatct
     3541 caagtatgaa taaagtattt aagcttaact tatgaagtct gttttattca cgttactcag
     3601 tcttgttttt acaacaactg tttatgcaca gcctgccggc acccgaacta taacaattaa
     3661 cgattgtaat tatcctatat taggtattga aaccaatcac gcctctgata agatagtttt
     3721 aacggcctca caaggacgta tcttttttgt taatgtaagt gatgtagaag ctaacactga
     3781 agtaagccct gtttggaaaa atttaagcct ttccggatta aacaatggag gtaagcctca
     3841 atttagtgag aacgatcaat atatattgtt acaggaatca tctgcattga agactgcaat
     3901 tacatcaaga aaagtaaaag atgttaaata ctatatttta aatgccgctg atggaaaagt
     3961 tgttgctgaa ggaaacaatg taaactcagt acagttttta aacaacagca cacaaatctt
     4021 aatatcatca aatgaaggta ttgtggttaa agattttttg acaaataaaa tagtagctca
     4081 gttgaaagta gaagactgtg aaatggcagc tatcagccac gacagcaaat taattgcact
     4141 ttcgtttgat cctgataaag ctgcatttaa agaagtagaa tctattggta agaataaaag
     4201 tgaactaaaa gctgccagaa agaacaagcg actaattagt ttttacagtt atcccgattt
     4261 aaagaaactt tataattcaa aagaagaaat agatgttgtt tattcaataa aattctctaa
     4321 agatgataaa catacctatt tgttatcaca taatgtcata gcagagcgaa ttggaacagg
     4381 tgctttaaat ggcctgtcaa tggctgctgc tgataatcgg agacaaacaa acatacaggt
     4441 gttagatgca agcaatggca acttggttaa agaaatttat catgccacag cagagcctga
     4501 tgcagattat aagtttagtg cttactctaa ctatttcggc tattctgaca atgatggttt
     4561 tataggatgg aaacgaaagc tcgtattaat agataataac gatcataaaa acaaagttgg
     4621 tcaataccgt tatcagggca ggagaggaag ctcaaatatt tttccgatat atttttcttt
     4681 taacaaaaca aagcccgttg cctacctatc aaatggtaca aagcttatag aatggaatta
     4741 tgaaacccta aaagaatata cagattatat tgaagggcaa gataacgact ccttgcaatc
     4801 attagcaatt aatcagt
//
LOCUS       DAOQNP010000122         4803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_7507_length_4803_cov_9.558130, whole genome shotgun sequence.
ACCESSION   DAOQNP010000122 DAOQNP010000000
VERSION     DAOQNP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7507_length_4803_cov_9.558130"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="purQ"
                     /locus_tag="PKY09_11935"
     CDS             <1..431
                     /gene="purQ"
                     /locus_tag="PKY09_11935"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNB13603.1"
                     /translation="FQILCESQLLPGALLHNNNHKFICKNVFIKAETDETILTSSLKI
                     GSAIKIPIAHGEGRFYADEVTLNQIESNNQVLFRYCDENGYVTEAANPNGAINNIAGI
                     CNEKQNVFGMMPHPERAADTLLGNTDGLGIFKSILNYVDA"
     gene            complement(447..569)
                     /locus_tag="PKY09_11940"
     CDS             complement(447..569)
                     /locus_tag="PKY09_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13604.1"
                     /translation="MNKEKVILWFKRLGLAGFLFFLIKGLIWLAVLYGAVEWFK"
     gene            complement(648..2744)
                     /locus_tag="PKY09_11945"
     CDS             complement(648..2744)
                     /locus_tag="PKY09_11945"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNB13605.1"
                     /translation="MAIIGKIRSKAGLLIGIIGLSLVAFILGDLLTSNRSFIQGNTTT
                     AGVIGGKKISIQDFEAKVQQNIENYKLSQGKDQVDQQTTDQLRDQTWGQMLNEELIGK
                     QIAKTGLVVSSDELFDMVQGNDIHPQIKQAFSDPNTKQFDKKNVIQFLKNMDNDQTGR
                     TRAQWVNFENSIKEERLNQKYFDLIKKGLYVTTAEAKRDFEAKNKTESARYIMLNYNT
                     IADSTVKLTDADLQKAYNENIKSYEQPEETRKLDYVTFDVVPSNEDRTVAEKYIQDLV
                     PAFTASTDDSLFVAQNSDFPVDNSYHKTGTLSPAIEAQMFAEANGFVSAPYMENNAFY
                     VAKLEGMKMLPDSVQARHILFKTEGKDSLHVKATADSIYNLIKGGAKFDQFVMLSEDP
                     GSAMKGGDLGWFKPGMMVPEFNDFCFDNKKGSLGMVKTNFGYHIIDITDQAGMSKNVR
                     VYVVGRKIEASSKTYQGVFAKANDFAAKNNNGDSYTKAVKDEGLNARNIENLKENDKT
                     VPGLDNARELIRWAFKAKKNDVSIPFELGDKFVVAHLIQIKPEGTLPLEDVKEQVEVE
                     ARKNKKAEMLIEKINATGAAQSLDGLASKLNIAVQPLENVTFNSPYIPNLGAEMNVIG
                     TMFGIGKDKVSKPIKGEQGVFVVQVTDVKEPQAPANFNESKTQAEQQLQNRSQYETFN
                     ALKEKAKVKDYRGKFY"
     gene            complement(2830..3666)
                     /locus_tag="PKY09_11950"
     CDS             complement(2830..3666)
                     /locus_tag="PKY09_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNB13606.1"
                     /translation="MNRFKNILINGKHGKPVVADMFFNGNKDPMPVIIFAHGFKGFKD
                     WGPYDMIAESFAHAGFVFIKFNFSFNGTTPDNPTEFADLEAFGQNNFTKELDDLDTLL
                     NWLWQHPQKERFDLNTIYLAGHSRGGAISIIKAAEDGRISKIASWASPIEFGKYISPQ
                     QLSLWQQAGVIYIENARTHQQMPLYYQLAEDAINNAHRINIHKAVKQLSIPHLIIHGT
                     EDETVSFSDALVMHSWNTRACLTPIASANHSFGAEHPYAGQLLPPHYQQVVNETIAFF
                     RA"
     gene            complement(3756..4169)
                     /locus_tag="PKY09_11955"
     CDS             complement(3756..4169)
                     /locus_tag="PKY09_11955"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNB13607.1"
                     /translation="MKKVIIFGFIFLTSVVAMAQSGEVFTTVEKMPEFPGGQTGLQEY
                     LLKNIKYPEEARTNVVEGVSYINFVVSSTGEVTGARVLKGNNEQFNQEALRVINAMPR
                     WQPGMQAGQTVSVQYTLPVRFSLTVPDKEEKKHKK"
     gene            complement(4203..>4803)
                     /locus_tag="PKY09_11960"
     CDS             complement(4203..>4803)
                     /locus_tag="PKY09_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNB13608.1"
                     /translation="KFEALAAHDAIVEAHGALKTVACSLLKIANDIRLLASGPRCGIG
                     EVYIPDNEPGSSIMPGKVNPTQCEAMTMVAAQVLGNDVAINIGGATGHFELNVFKPVM
                     IYNFLHSARLIGEVCVSFNDKCAIGIAPLHDNIKKNLENSLMLVTALNTHIGYYKAAE
                     IAQTAHKQGKTLKQTAIDLGYVTSEQFDAWVKPEDMVGL"
BASE COUNT         1377 a          999 c          862 g         1565 t
ORIGIN      
        1 gctttcagat tttatgcgaa tcgcagctgc taccgggagc cttgttgcac aataacaatc
       61 ataaatttat ctgtaaaaat gtttttataa aagcagagac cgatgaaacc atccttacct
      121 catcactgaa aatcggatcg gctattaaaa ttcctattgc ccatggcgaa ggacgcttct
      181 atgcagatga agtaacatta aatcagatag aatcaaacaa tcaggtgctt ttccgctatt
      241 gcgatgagaa tggctatgta acagaagctg ctaatcccaa tggtgccata aacaatattg
      301 caggtatctg taatgaaaaa caaaatgttt ttggaatgat gccgcatcct gagcgtgcag
      361 cagacacctt attgggtaat acagatggac ttggaatttt taagtctatt ctcaactatg
      421 ttgatgcctg actttattaa gcacaattat ttaaaccact ctactgcacc ataaagtact
      481 gcaagccaga taagaccttt aatcaggaaa aataaaaatc ctgcaagccc cagcctttta
      541 aaccacaata tgactttctc tttattcaca ccccaaaaat attaagggct tcggtgattt
      601 ttgttccgaa gcccttattt ttttaagtaa aatctttttt acaattctta ataaaactta
      661 cctctgtagt ctttaacctt tgctttttct ttcaatgcat taaaggtttc gtactgcgaa
      721 cggttttgca attgctgctc tgcctgagtt ttgctttcat taaaattagc aggtgcctgg
      781 ggctctttta catctgtaac ctgaacaaca aatacgcctt gctcaccttt aattggctta
      841 ctcaccttgt ctttacctat tccgaacata gtaccaataa cattcatttc tgcgcccaaa
      901 ttaggaatgt atggagaatt aaaagtaaca ttttccaatg gctgaacagc aatatttagc
      961 tttgatgcca atccgtcaag actttgtgct gcaccggttg cattgatttt ttcaatcagc
     1021 atttctgctt ttttgttttt gcgtgcttca acctctacct gctcttttac atcttccaaa
     1081 ggtaatgtac cttcaggttt tatctgaatt aaatgtgcta caacaaattt atcacccaat
     1141 tcaaaaggaa ttgaaacatc gttcttcttt gccttaaagg cccagcgtat taactcacgc
     1201 gcattgtcaa ggccgggaac agttttatca ttttctttta aattttcaat gttacgggca
     1261 tttaatcctt cgtcttttac agctttagta tagctgtcgc cattattatt ttttgctgca
     1321 aaatcatttg cctttgcaaa tactccctga taggttttag agctggcttc aattttacgt
     1381 ccaacaacat aaactctgac attttttgac atgcctgcct gatcggtaat gtcaataata
     1441 tgataaccaa aatttgtttt taccattcct aacgatcctt ttttattatc aaaacaaaag
     1501 tcgttgaatt caggaaccat cattcccggt ttaaaccaac ccaagtcgcc acctttcata
     1561 gcagagcccg gatcttcaga aagcataaca aactgatcaa atttagcacc acctttaatc
     1621 aggttataaa tactgtcggc agtagcttta acatgcaatg aatcttttcc ttctgtctta
     1681 aataaaatat gacgtgcctg aacagaatca ggaagcattt tcattccttc taattttgca
     1741 acatagaaag cattgttttc catatacgga gctgacacaa aaccattagc ttctgcaaac
     1801 atttgtgctt caatagcagg tgaaagtgtt cctgttttat ggtagctgtt gtcaacggga
     1861 aaatcagagt tctgtgctac aaacaaagag tcgtctgtgc tggcagtaaa tgcaggtacc
     1921 aagtcctgaa tgtatttttc tgctacagtt ctgtcttcgt tagatggcac aacatcaaaa
     1981 gtcacatagt caagtttgcg tgtttcttca ggctgctcat aacttttaat gttttcgtta
     2041 tatgcttttt gcaaatcggc atcagtaagt ttcactgtgc tgtcggcaat ggtattgtag
     2101 ttaagcataa tataacgtgc agattctgtt ttgttcttag cttcaaagtc gcgctttgct
     2161 tctgcagtgg ttacataaag acctttttta atcaggtcga aatatttctg attcagtctt
     2221 tcttctttaa tgctgttttc aaagtttacc cactgtgctc ttgttcggcc tgtctgatcg
     2281 ttatccatat ttttcaaaaa ctggattaca tttttcttgt caaactgctt agtgttaggg
     2341 tcgctgaatg cctgttttat ctgaggatga atgtcattcc cctgaaccat gtcaaataat
     2401 tcatcactac ttacaaccaa tccggtttta gcaatttgtt tgcctatgag ctcttcgtta
     2461 agcatctgcc cccaagtctg atcacgtaat tggtctgttg tctgttggtc aacctgatct
     2521 tttccctgac tgagtttata gttttctata ttctgttgaa ctttggcttc aaagtcctga
     2581 atagaaattt tctttccacc aataacacct gccgttgtgg tgtttccctg aatgaatgag
     2641 cggttagaag tgagcaagtc gccaaggata aaagctacta agcttaatcc gatgatacct
     2701 atgagcaggc cggctttact tcttattttt ccaataatag ccataatgtc ttaaataatg
     2761 ggggtttaaa aatgaggggg cgaatataca actgaaaaag caataaataa aacgaaattt
     2821 ttagagatat caggctctga aaaacgctat tgtttcgttt acaacttgct gataatgagg
     2881 tggcaataat tgtccggcat aaggatgttc ggcaccaaaa ctatgatttg ccgaggctat
     2941 tggtgtcaga caggctcgtg tattccacga atgcatgact aaagcatcac taaagcttac
     3001 tgtttcatcc tctgttccat gaatgattaa atgaggtatt gaaagctgtt ttactgcctt
     3061 atgaatattt attctatgcg cattgttgat ggcatcttca gcaagctgat agtagagcgg
     3121 catttgctga tgggttcgtg cattttcaat ataaatgact cctgcctgct gccacaaact
     3181 aagctgttgt ggagaaatat acttaccaaa ctcaataggc gatgcccatg atgcaatctt
     3241 gctgatacga ccatcttctg cagctttgat tattgagata gctcctccgc gagagtgccc
     3301 tgcgagataa atagtgttta aatcgaaacg ctctttttgc ggatgctgcc aaagccagtt
     3361 cagcagagta tccaaatcat caagctcttt ggtgaaatta ttttgtccga atgcctcaag
     3421 gtctgcaaac tctgtcgggt tatcgggtgt agttccgtta aacgaaaaat taaatttgat
     3481 aaagacaaat cctgcatgag caaacgattc tgcaatcata tcataaggtc cccagtcttt
     3541 gaagccttta aagccatgag caaaaataat tacaggcata gggtctttgt tgccattgaa
     3601 aaacatatcg gccacaacag gtttgccgtg ttttccattg atgaggatat ttttaaagcg
     3661 gttcattgta ttaaaatatg agagcacaat ttacaaaaaa acaaaacagc tgtcaatgaa
     3721 gttttgacag ctgttttaaa aatgatgtta ttaatctatt ttttatgctt tttctcttct
     3781 ttatcaggca ctgtcaatga aaaacgtaca ggtaaagtat attgcacact tacagtctgt
     3841 ccggcctgca tgccgggttg ccagcgtggc atggcattaa taacacgcaa agcttcctga
     3901 ttaaactgct cattgtttcc tttcaaaacg cgggcaccgg taacttctcc ggttgacgac
     3961 accacaaagt ttatatacga aacaccttcc acaacattgg ttcttgcctc ttctggatat
     4021 ttgatgtttt tcagaagata ttcctgtaag cctgtttgtc caccgggaaa ttcaggcatt
     4081 ttttccactg tagtaaatac ctcacccgac tgcgccattg ccacaaccga agtgagaaaa
     4141 ataaatccga aaatgataac ctttttcata aactattatt ttaatcgtaa aaataacaaa
     4201 aattataatc ccaccatatc ttcgggtttc acccaggcat caaactgctc tgatgtaaca
     4261 taacccaaat caatagctgt ctgctttaaa gttttgcctt gcttatgtgc tgtttgtgca
     4321 atctcagcag ctttgtagta gccaatgtgt gtgttaagtg cagttaccag catcaaagag
     4381 ttttccagat tttttttgat attgtcatgc agtggtgcaa tgccaatggc gcatttgtcg
     4441 ttaaatgaaa cacacacttc accaattaat cttgcactgt gcaggaagtt ataaatcatt
     4501 accggcttga aaacattcag ttcaaaatgt cctgttgcac cgccaatgtt tatggctaca
     4561 tcattaccca acacctgtgc agctaccata gtcatggctt cgcactgagt aggatttacc
     4621 tttcccggca tgatagaaga acccggctca ttgtcgggaa tatatacttc gccaatgcca
     4681 caacgggggc cactggcaag taagcgaata tcattggcta ttttcaacag agagcaggca
     4741 acagttttta atgcaccatg tgcttcaaca atcgcatcgt gtgctgctaa tgcttcaaat
     4801 tta
//
LOCUS       DAOQNP010000123         4734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_7678_length_4734_cov_6.455225, whole genome shotgun sequence.
ACCESSION   DAOQNP010000123 DAOQNP010000000
VERSION     DAOQNP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7678_length_4734_cov_6.455225"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..>2504
                     /locus_tag="PKY09_11965"
     CDS             227..>2504
                     /locus_tag="PKY09_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNB13609.1"
                     /translation="MKSHHYNFPKAPFNEAMLVIDFFDTQASKTPNQIAVYFHEEKLT
                     YVQLHKQAGILAQFLINKGVKPGDLVGLCIERSCNLIVGVLGILKAGAGYVPLDPEYP
                     QERLQYMIETAQMPLLITNKTLSEKIPASKAEKILIEEIFEKHSSPLAQTITHAPTDI
                     AYVLFTSGSTGMPKGVAMPHRPLVNLITWQLKNTKVLDSAKTLQFAPISFDVSFQEIF
                     STLSCGGTLVMIEDELRLNAVNLLNFVTEEKINRLFLPFIALQHLCEVAENFEGDLSC
                     LQEVITAGEQLQSTQFIRNFFKKNKNCTLWNHYGPTEGHVVTAYLLSSNPDTWAALPS
                     IGKPIDNAEIYILNDDGNVVADNEEGELYIGGVSVAKGYLNRQDLTQERFLENPFNKT
                     QGKYFYKTGDLARYMPDGNIEYLGRIDGQVKVRGYRIELGEIELTLDKHVSVKQAVVI
                     AREDEPGDKRLVAYCLPNDNKKILAGELRQFLQDKLPEYMMPSAFVQVENLPRTPSGK
                     IDRRGLPAPSNQRPDLSTVFVAAETDLQKTFVKIWSKLLKVDPIGINDNFFDLGGNSL
                     LALKFIAALRQEHNLDLPVVKMYQHPDIYSLTEFLTNGSVAKSFYENAQDRFSSNIKG
                     KIETVEDGIAVIGMAGRFPGANNVDEFWKNIVEGKETVSFFKKHELDSAVPDEDKNDE
                     NYVAVRGIIEDADKFDAPFFNINPRLAEVTDPQQRIFLQVAWEAIENAGYAPDKYNGL
                     IGVFAGMGNNTYFPNNVFH"
     assembly_gap    2505..2604
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2605..>4734
                     /locus_tag="PKY09_11970"
     CDS             <2605..>4734
                     /locus_tag="PKY09_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HNB13610.1"
                     /translation="GVFAGMGNNTYFPNNVFHNKEAINRVGSFQTMTANEKDYIATRI
                     AYEFNTKGPAVSVHTACSTSLTAVTLAYESLLNHECDMALAGGVAITVPVNSGHVYNE
                     GGMFSKDGHTRAFDAGANGTVFSDGAGAVILKRYKDAIKDGDEIYAVIRGAALNNDGS
                     EKASFTAPSIEGQAAVIAMAQAKAGVSPDSISYIETHGTATPLGDPIEIEALTQAFRS
                     KTDKKQFCAVGSVKTNIGHLTPAAGVAGLIKTCLALKNKILPASLNYSKANPAIDFEA
                     SPFYVNSTLKIWTTENGEPLRAGISSFGVGGTNAHVVVEEAPQKVESGIARKKHLFLL
                     SGRTKDALDLQTKNLQSFLEKNPNLNLADIAYTLQTGRKYFNHRRIISGGDVQEIIAT
                     LQQANPKKTTARELITGSPEIIFMFPGQGSQYVNMGKNLYDDEIVFKDAVDTCCNILQ
                     PHLGIDLRTILYPEAVEKEKAEKLLKETIYTQSALFTIGYALAKLWMSWGIQPKGFIG
                     HSIGEFAGAHLAGVFSLEDALMLVANRGRMMQELPHGSMLSVRKPAAEMEKEIDSSCS
                     IAAINGPALCVVAGPTEKIEALQKSLESREITAKLLVTSHAFHSPMMEPMLKPFLEKI
                     KSVKLNKPTIPFVSTATSQWISDAEATNPEYWMNHVRATVRFAEGIQTLWSDKPQRVL
                     LECGPRNTAATLAKQQAMAERIASASEQ"
BASE COUNT         1531 a          877 c          941 g         1285 t
ORIGIN      
        1 aaaagcagaa gccctcacgg ttatagcgga caaaggttat cacacaggaa aggaactgca
       61 agcttgaaat tctgactaat ttgtcggata aagtcctgat actttaacta agttcccatt
      121 ccattcacca aaattatatg aatgatacaa cgaaacttat tacattcgtc tccgtacaat
      181 acagttgtac agagcggcac ataatagtta ttaaaccacc aacttaatga aatcgcacca
      241 ttataatttt ccgaaagcac cctttaacga ggccatgctt gtgatagact tttttgacac
      301 tcaggcatct aaaacaccta atcaaattgc cgtatatttt catgaggaga aattaactta
      361 tgtgcagcta cataaacaag ccggaattct tgcacaattt ctgataaaca aaggggtaaa
      421 acccggtgac cttgttggtc tttgtattga acgcagttgt aatttaattg ttggtgtatt
      481 gggtattctg aaagccggtg ctggatatgt cccgcttgac cctgaatatc cgcaagaacg
      541 attgcaatat atgattgaga cagctcaaat gccactgctc atcactaata aaacgctttc
      601 ggaaaaaata cctgcaagca aggcagaaaa aattttaatc gaagaaattt tcgaaaaaca
      661 ttcttcacca ctcgctcaaa caataacaca cgctcctact gacattgctt atgtactttt
      721 taccagtgga tcaacaggca tgcccaaggg tgttgccatg ccacacagac ctttggttaa
      781 tctgattaca tggcaattga agaacaccaa agttttggat tctgcaaaaa cattacaatt
      841 tgctcccatt agttttgatg tttcatttca ggaaattttt tctacactaa gttgtggtgg
      901 caccttggtg atgattgaag atgaactgcg attgaacgct gtaaatcttt taaactttgt
      961 tactgaagaa aaaatcaatc gtttgttttt acctttcatt gctttacaac atctatgtga
     1021 agtagctgaa aattttgaag gcgatttaag ctgcctgcaa gaagtgatta ctgcaggtga
     1081 gcaattacag tcaacacagt ttatcagaaa cttttttaag aaaaataaaa attgtacact
     1141 ttggaatcat tacggaccaa ctgaagggca cgttgttaca gcatacctgc tttcttcaaa
     1201 tcccgacaca tgggctgcac tgccttccat cggcaagcca atagataatg cagagattta
     1261 tattctgaat gacgatggaa atgttgttgc agataatgaa gaaggcgaac tttacattgg
     1321 tggtgtaagc gttgcaaaag gttatctgaa tcgccaggac ttaacacaag aacgatttct
     1381 tgaaaaccct ttcaataaaa cacagggaaa atatttttat aaaacgggcg accttgcacg
     1441 ttatatgcct gatggtaata ttgaatacct cggaagaatt gacggacaag taaaagtcag
     1501 aggctataga attgaactgg gtgaaattga attgactctc gataaacatg tatcagtaaa
     1561 acaagctgtt gtaattgctc gtgaagatga gcccggtgat aaacgtttgg ttgcatattg
     1621 cttacctaat gacaataaga aaattttagc cggagaactt cgtcaattcc tgcaagataa
     1681 attacctgag tatatgatgc cttcggcatt tgttcaggtt gaaaaccttc cacgtacacc
     1741 ttcaggaaaa atagaccgca gaggtttacc tgcaccatcc aatcaaagac cagatttaag
     1801 cactgtattt gttgctgctg aaacagactt gcaaaaaact tttgttaaaa tctggagtaa
     1861 gttattaaaa gttgatccga ttggcattaa tgacaacttt tttgatcttg gcgggaactc
     1921 acttctcgca ttaaaattta tagcagcatt aagacaggag cacaatttag atcttcctgt
     1981 tgttaaaatg tatcagcatc ctgatattta ttctctgaca gaatttttaa ccaacggatc
     2041 tgtagcaaaa tcattttatg aaaacgcaca agacagattc tcttcaaaca taaaaggtaa
     2101 gattgaaacg gtagaagatg gtattgccgt aattggtatg gcaggacgtt tccccggtgc
     2161 aaacaatgta gatgagttct ggaaaaatat tgttgaaggt aaagagactg tctcattttt
     2221 caaaaaacac gaattggata gtgcagtgcc tgacgaagac aaaaacgatg aaaactatgt
     2281 tgcagttcga gggatcattg aagatgccga caaatttgat gcaccatttt tcaatataaa
     2341 tccacgtctt gcagaggtca ccgatccgca acagcgcatt tttttacagg tggcatggga
     2401 agctattgaa aatgcagggt atgcacccga caaatacaat ggacttattg gagtttttgc
     2461 aggaatggga aacaacacct acttcccgaa taatgttttt catannnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnggagtt tttgcaggaa tgggaaacaa cacctacttc
     2641 ccgaataatg tttttcataa taaagaagca attaaccggg taggctcatt tcaaacaatg
     2701 actgccaatg aaaaagacta tattgctaca agaattgctt atgagtttaa tacaaaaggg
     2761 ccggctgtaa gtgtgcatac ggcatgttca acatcgctca ctgctgttac acttgcctat
     2821 gaaagtttat taaaccatga atgtgatatg gcacttgccg gaggtgtggc cattactgtc
     2881 ccggtaaaca gcggacacgt ttataacgaa ggcggcatgt tcagtaaaga cgggcataca
     2941 agagcatttg atgcaggtgc caacggaaca gtctttagtg atggtgcagg tgctgttatt
     3001 ctgaaaagat ataaagatgc aataaaagac ggtgacgaga tttacgcagt tattcgaggt
     3061 gcagccttaa ataatgatgg cagtgaaaaa gcaagcttta ctgcaccaag tattgaaggt
     3121 caggctgcag ttattgctat ggcacaggcc aaagcaggag tgtcgccaga ttctatctca
     3181 tatattgaaa cacatggtac tgccacacca cttggtgacc caatagaaat tgaagcactc
     3241 actcaggcat tcagaagcaa aactgataaa aaacaatttt gtgctgttgg atcggtaaag
     3301 accaatatcg gacacctgac accggcagca ggcgttgcag gcctcattaa aacatgtctt
     3361 gcactaaaaa acaaaattct accagcttca ttaaattatt caaaagcaaa tccggcaatt
     3421 gattttgaag cttcaccatt ctatgttaat tccactttaa aaatttggac aacagaaaat
     3481 ggtgagccct tgcgtgccgg tatcagctct tttggtgttg gcggaactaa tgcacatgtt
     3541 gtagttgaag aagcaccaca aaaagttgaa tcaggcattg cacgtaagaa gcatttgttt
     3601 ttactttcag gaagaacaaa ggatgcattg gatttacaaa caaaaaacct gcaatcgttt
     3661 ttggagaaaa atcctaatct aaatcttgca gacattgctt acactttaca aacaggacga
     3721 aaatatttta atcatcgcag aattatttca ggtggtgatg tgcaagagat tatagctaca
     3781 ttacaacaag ccaatcccaa gaaaacaact gcacgtgaac tgataacagg cagtccagaa
     3841 atcattttta tgtttccggg tcaaggttca cagtatgtaa acatggggaa aaatttgtat
     3901 gatgatgaaa tagtttttaa agatgctgtt gacacatgct gcaatatttt gcaaccacat
     3961 ttaggcattg atttgcgtac aattctttat cctgaagctg ttgaaaaaga aaaagcagaa
     4021 aagcttttaa aagaaacgat ctatactcaa tctgcattat ttacaatcgg ttatgcattg
     4081 gcaaaacttt ggatgagctg gggcattcaa ccaaaaggct ttataggtca cagtatagga
     4141 gaatttgcag gtgcacattt ggcaggagta ttttctttag aagatgcact gatgcttgtt
     4201 gccaatcgcg gacgaatgat gcaggagtta ccgcatggaa gcatgttaag tgtccgcaaa
     4261 ccggctgcag agatggaaaa ggaaattgac tcctcttgct ctattgctgc aattaacggt
     4321 cctgcacttt gcgtagttgc cggcccgaca gaaaaaattg aagcacttca aaaatcattg
     4381 gaaagtcgcg aaataacagc aaaattatta gtgacatcac atgcatttca ttctccgatg
     4441 atggagccaa tgctgaagcc ctttcttgaa aaaataaagt cagtaaaact caataaacca
     4501 acaataccct ttgtttcaac tgcaacttcc caatggattt cagatgctga agcaacaaat
     4561 ccggaatact ggatgaacca tgtcagagca actgtgcgct ttgctgaagg tattcaaact
     4621 ttgtggagtg ataaaccaca acgtgttttg ttggaatgcg ggccaagaaa caccgctgct
     4681 accttagcca aacagcaggc aatggccgaa agaattgctt ctgcttcgga acag
//
LOCUS       DAOQNP010000124         4651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_7936_length_4651_cov_7.326588, whole genome shotgun sequence.
ACCESSION   DAOQNP010000124 DAOQNP010000000
VERSION     DAOQNP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7936_length_4651_cov_7.326588"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1455)
                     /locus_tag="PKY09_11975"
     CDS             complement(124..1455)
                     /locus_tag="PKY09_11975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB13611.1"
                     /translation="MNKLYKYFAIGAAMLSLHGVALAQGTKEVNVDGLKLILRHTPKE
                     VISVRLYVKGGTANYAKEKEGVESLAFSIAMNGGTKKLDKVAFNDAAEKIGTSFESSS
                     GLDYGNLSMTCIKPNWNESWNLFADAVMNPAFDSKEFDLEKEKALSGAKQRESDADGS
                     LSSIAMEETFKGRNYAKDPQGSVASLEKLTLADIISHYKNVIGKKRCYLVVVGDVNEV
                     DLVNKIKATLGKMPVGTAPVVEPKLVITKGSEVIVDRDIATNYLMGIMSAPSLSTEKG
                     VQMDLAMQMLYDRFFVELRTKRSLSYAPAAGYMRNAVSSPYSYIYITTTDPKKAIDVM
                     VSIIDSIKKTGFTEKELKDLRQGYLTNYYQKLETSEAQSGALGRSEATGSWKLDESLS
                     DMVNNVKLKDLNNTFNEYTAAIKWSYLGKKDAISKDDFKQTKHEKVLQSPY"
     gene            complement(1468..2838)
                     /locus_tag="PKY09_11980"
     CDS             complement(1468..2838)
                     /locus_tag="PKY09_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNB13612.1"
                     /translation="MLKKITAFATALIIAGTAMAQKNLPANFHMKKLGNGLEVLVIED
                     NSVPLVTIEICVKNGSFTEDSAYNGLSHLYEHMFFKANKAIPSQEKFLERVNELGISF
                     NGTTSEERVNYFFTLSNKLINEGLEFMNNAIRYPLFLEQEMKNENPVVDGEFQRAESN
                     PAFFLFQDFNRQMWGSNYVRKNAIGIHDVILSATPEKMNVIKDKYYYPNNSILVVAGD
                     VEHNAVFEKVDKMFGDWKPCDFDPFQKWPIPEFAPLVGQSKFLTVDKNAKMPIMMMGF
                     HGPDTRNDVKSTYAADVFSTILGLTSSEFQKTLVDGGYALQAGLGYQTCRYTGPIQLF
                     MVPNPMKIKSFYGKLMEQVNRFDAPDYFTDEQLETAKNKLIMDDIYNKEKTSAYVHTV
                     TFWWASASIDYYTTYNDMIKKVTRKDIAEYVDKYIKGKPSVTGLLLNPKMKESLGINS
                     ADEILK"
     gene            complement(2920..4479)
                     /locus_tag="PKY09_11985"
     CDS             complement(2920..4479)
                     /locus_tag="PKY09_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13613.1"
                     /translation="MRKFLHTCLITGLSFFTLQSMAQMQYPGIIDSTFGVNGKVTTSF
                     NSFACVARAIAVQSDGKIVVAGESNNGTFNRFAVARYLADGTIDNQFGTGGKITFSIG
                     TGNSIARSIAIQNDGKIIVAGNLENQTYSHFAMARLTSTGALDNQFGANGIVQTALDL
                     GFDIAYGVAIVPSTGKIVLGGVSFDTLANGEFAVVRYNTNGTLDGGFGNSGKVRTPLG
                     TQWDQANALAIEPGTEKIVLGGRTLSTYAMVRYNTNGTLDANFGLNGKTIYTIGSMAE
                     VINTIYINPDGKIVAGGTTQTGSNTDMVLMRFLNNGQIDNNFGANGISLFDLGGTDII
                     FSIVPQGDYLVGAGSSYVGAAGDFVAMRFDTLGSLDLLFNNMIGFNITSFGNPSGANA
                     VAIQQPDSKIILAGLAVVGTVNNFAMARYTSDFTFVGLNELNTSHAKLQMMPNPASDF
                     VAIESSTPYNRIEIKDITGKVVKVVTPGSLIRSYSLDVKNYSSGVYTATVYFDTGIKV
                     QKFIVANSINN"
BASE COUNT         1442 a          935 c          740 g         1534 t
ORIGIN      
        1 cacaaatcac ctatgcaaac atgtagtcag ctttttccgg atgaaaatta caatgagcgg
       61 tatatataaa aaacgagcgg cacgcaattg cgtgccgttc gtttttatga ttcaatctga
      121 ttattaatac ggactttgca acactttttc gtgtttggtt tgcttaaaat catcttttga
      181 tatggcatct ttctttccga gataagacca tttaatggct gctgtatatt cattgaatgt
      241 attattcaaa tctttcagtt taacattatt aaccatatca gaaagagatt catctaactt
      301 ccatgagcca gttgcttcgc tacgccccaa tgcacctgat tgtgcttctg aagtttctaa
      361 cttctgataa taatttgtaa gatatccctg acgcaaatct ttcagttcct tttctgtaaa
      421 tcccgtcttc ttaatagaat caataataga aaccattaca tctattgctt tcttaggatc
      481 tgtggttgtg atatagatat aagagtaagg tgaggaaaca gcattgcgca tgtagcctgc
      541 agcaggtgca taagacaaac tacgttttgt tctaagttca acaaaaaatc tgtcataaag
      601 catttgcatt gctaaatcca tttggacacc tttctctgtt gacaatgaag gagcactcat
      661 tattcccatc aagtaatttg tagcaatatc gcggtcaaca ataacttcac tacctttagt
      721 aataactaat ttgggttcca caaccggtgc tgtgccgacc ggcatttttc ccaatgttgc
      781 ttttatctta ttcactaaat ctacttcgtt aacatcacct actacaacta agtagcaacg
      841 cttttttcca atcacatttt tgtaatggct aataatatct gccaacgtta atttttcaag
      901 gcttgcaaca gagccttgcg ggtcttttgc atagtttctg cctttgaatg tttcttccat
      961 agcaatggaa ctcaatgaac catcagcatc agattcacgt tgctttgctc cggataatgc
     1021 cttttctttt tccaaatcaa actcttttga atcaaaagca ggattcatta cagcatcagc
     1081 aaaaagattc catgattcat tccaatttgg cttaatacat gtcatactca ggttgccata
     1141 atccaatcct gatgacgatt cgaatgatgt accaattttt tcggcagcat cattaaaggc
     1201 aactttatcc aatttttttg tgccgccatt catagcaata gaaaatgcaa gactctctac
     1261 accttctttc tcttttgcat aattagccgt tcctcctttt acatataacc gcacgctaat
     1321 aacttctttt ggtgtatggc gtaatattaa tttcagacca tcaacattaa cttcttttgt
     1381 tccttgtgca agggcaacac catgtagtga taacatagct gcaccaattg caaaatattt
     1441 atataactta ttcatagttc gattttttta ttttaaaatt tcatcagcag aattaattcc
     1501 aagagactcc ttcattttag gatttaagag cagacctgta acagaaggtt ttcctttgat
     1561 atatttatca acatattctg caatatcttt tctggtaact ttcttaatca tgtcattgta
     1621 ggtcgtatag taatcaatgc tggcagatgc ccaccagaat gtaacagtat gcacataggc
     1681 tgatgttttt tctttgttat aaatatcatc cataatgagc ttattcttag ctgtctccaa
     1741 ctgttcgtca gtaaaataat caggtgcatc aaaacgattt acctgctcca tgagtttccc
     1801 ataaaatgat tttatcttca ttggattagg aaccataaat aactgaatag gtcctgtata
     1861 gcgacaagtc tgataaccta aacctgcctg aagtgcatag ccaccatcaa ccaatgtttt
     1921 ttgaaattca gaagatgtca atccaagaat agtagaaaac acatctgctg cataagttga
     1981 cttaacatca tttcttgtat caggtccatg aaatcccatc atcataatag gcatttttgc
     2041 atttttatct acagtcagaa attttgattg tccaactaaa ggggcaaatt ccggtattgg
     2101 ccatttctga aatggatcga aatcacatgg tttccaatca ccgaacattt tgtccacctt
     2161 ctcaaaaaca gcattatgtt ccacatcacc tgcaaccact aaaatagagt tgtttggata
     2221 ataatattta tctttaatta cattcatctt ctcaggtgtt gcagaaagta ttacatcatg
     2281 aataccgata gcatttttac gtacataatt agaaccccac atttgtctgt taaaatcctg
     2341 gaacagaaaa aatgcaggat ttgattctgc acgttgaaat tcaccatcaa caacagggtt
     2401 ttcatttttc atttcctgtt caaggaataa tggataacgg atagcattat tcataaactc
     2461 aagaccttca ttaatgagtt tgttacttag tgtaaaaaag tagttcacac gctcttcgga
     2521 ggtagtacca ttaaatgata taccgagttc attaactctt tcaagaaact tttcctgaga
     2581 tggaattgcc ttgtttgctt taaagaacat gtgttcgtaa agatgtgaca atccattgta
     2641 ggcagaatct tctgtaaagg atccattctt tacacaaatt tctattgtca ctaatggaac
     2701 agagttgtct tctatgacca agacctcaag tccattgccc aactttttca tatgaaagtt
     2761 agcgggaagg tttttttgtg ccattgctgt accggctata ataagagcag tagcaaaagc
     2821 tgtgattttt ttaagcatag ttatacattt aataagtggc taagaacgct atttcttttg
     2881 cctgtgatgg ttaaaaaaaa gttatgttat ttattaaatt tagttgttta tggaattcgc
     2941 cacaatgaat ttctgaactt taatgccggt atcaaaatat accgttgctg tataaactcc
     3001 tgatgaatag ttttttacat ccaatgagta gctgcgaatc aagcttccgg gagtcacgac
     3061 tttaactact tttccggtaa tgtcctttat ctcaatacga ttatatggtg ttgaagattc
     3121 tatagctaca aaatcagaag cagggtttgg catcatctgc agcttagcat gtgaagtatt
     3181 tagttcattt aatcctacaa aggtaaaatc gcttgtataa cgcgccatag caaaattatt
     3241 gaccgtgcca acaacggcta atccggcaag aataattttt gaatcaggtt gctgaattgc
     3301 aacagcattt gctcccgatg ggttaccgaa tgaggttata ttaaatccta tcatattgtt
     3361 aaaaagcaaa tcgagagaac ctaaggtatc gaaacgcata gctacaaaat cgccagcagc
     3421 acctacataa cttgaacctg cacctaccag ataatcacct tgaggaacga tagaaaaaat
     3481 aatatctgtt ccacctaaat caaagagaga aattccattt gcaccaaagt tattatcaat
     3541 ctgaccatta tttaaaaacc gcatcagtac catatctgta ttagatccgg tttgtgttgt
     3601 acctcctgca acaatttttc cgtcaggatt tatataaatt gtattgatga cttcagccat
     3661 cgagcctatg gtataaatag tctttccatt taaaccgaaa ttcgcatcta aagtaccgtt
     3721 ggtattatac ctcaccatag catacgttga tagtgtccgg ccaccaagaa caatcttttc
     3781 tgttcccggt tcaattgcca aagcatttgc ctgatcccat tgtgttccta atggagttct
     3841 caccttaccg ctatttccaa atccaccatc caaagttcca tttgtattgt atcttacaac
     3901 agcaaattcg ccattggcta acgtatcaaa cgaaacacct cccaaaacaa tttttccggt
     3961 ggatggaact attgctactc cataggcaat atcaaatcct aaatccaatg ccgtttgcac
     4021 aatgccgttt gcaccaaact gattgtctaa tgcacctgtt gatgttagtc tggccatggc
     4081 aaaatgtgaa taggtttgat tctcaaggtt accggcaaca attattttac catcattttg
     4141 tatggcaatt gaacgggcta tactgttacc tgtgccaata gaaaaagtaa tcttccctcc
     4201 tgttccaaac tgattatcaa ttgtgccatc agcaagatag cgggcaactg caaagcgatt
     4261 aaaagttcca ttgttacttt cgcctgcaac aacaattttt ccatctgact gcacagctat
     4321 tgctcttgca acacaggcaa aactgttaaa tgatgtagtt actttgccat ttactccaaa
     4381 cgtagaatca ataatgccgg gatactgcat ctgagccatt gattgtaatg taaaaaaaga
     4441 aaggcctgta atcaggcaag tgtgtagaaa ttttctcatt ctgaatgttt ttacttatga
     4501 tgcaaatata tcatttagcc tgaatgacaa ttattaattg tctaatattt ctagaaaaat
     4561 attgactaaa gtattctcaa aaaatctgct aaacttttac aaaacttatt tagcgaattt
     4621 aatgtcaata aaatataaaa aacaaccctg c
//
LOCUS       DAOQNP010000125         4558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_8243_length_4558_cov_8.847879, whole genome shotgun sequence.
ACCESSION   DAOQNP010000125 DAOQNP010000000
VERSION     DAOQNP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8243_length_4558_cov_8.847879"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1999)
                     /locus_tag="PKY09_11990"
     CDS             complement(<1..1999)
                     /locus_tag="PKY09_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNB13614.1"
                     /translation="MGNLKIYQSSAGSGKTYTLVKEYLLLVLKSPAALKGTLAVTFTN
                     AATGEMKERLILALWQLATGRNEKLKSDLASSLGTSFNIEQAAAKVLSYAIHHYTDIS
                     ISTIDSFFLNITRSIAYESRLPGRYEIDLNTEPAIDFMVQNILHAAGTDVDITNWLID
                     FAMHRVDSNKGWNMQGELKTIAKEVFKDHIRVKFETDVLADTSVINEVRGLHAAIWNK
                     MSGMAKKFLEIANSVGAESKDFYFGEKGFYSLIKKIAAKEHPKDNTINLRTWQILDRK
                     NVFADKAHDFLKQDQNLNSELLGIVRQIVDCYVESVEQLLSTEVVTTNIYQAVILKYL
                     FRQLKVYRKDHQMLLMSDVGKILNSIIGDNDTPFIYEKAGNKYRNFLIDEFQDTSQVH
                     WKNLLPLLINSLAEQHQVLTVGDGKQSIYRWRGGDMNLLLNEVESTLKNHANSTDVFR
                     LDSNFRSSVSVVEFNNIFFSAISEIISTDSQQSQLLKAAYQENTVHQIAKRSDERGFV
                     SIELFTKDESYSELHYPEYFSAADKVIASHTLSYIQSQIEKGYKPGDIALLVRSNING
                     NKLADFLVLNGYKNINSRDSLLICHAPQIRFLINCLRLNADNENQVAEKSILYFVSKH
                     LQMETKVIETCIEKYVKHSKLYSLTEFCTQVINKFGLNKQPD"
     gene            complement(2228..3388)
                     /locus_tag="PKY09_11995"
     CDS             complement(2228..3388)
                     /locus_tag="PKY09_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNB13615.1"
                     /translation="MNSLKSIISFSILTILLIFVSADGQSQQINNTSTKPRQVDVVIC
                     MDLSGSTNGLLDNFRDKMWDIINQWQMFTPKPVIRIGLIGFSRYSFHDGSGYIKVISD
                     LTDDYDALSQKMFDIQPYVENGIQFVGAAIGSGVNDMSWSEKPDAVKMMFVMGNGYVN
                     LGRFDFRAQCETAVKNNIEIHTVYCIKNDKQMRQRELPGWEEIAKLTGGQAFQVFVNN
                     RAPLIKEKVNVMALASLNDSLNKTFIPYGKTGVERLKLMLKADDHAVRSYEAYFYSRL
                     KFKLSDHYTSQMLMWDVTQYKEEYPQSRLAAVRQSVKSNNESSIDLELLAKEFVEKRN
                     SLRASINALFPLGEELRIHNEAVAKEYELENRLDKTMVRAYSQTLIKSGFKE"
     gene            3573..>4558
                     /locus_tag="PKY09_12000"
     CDS             3573..>4558
                     /locus_tag="PKY09_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNB13616.1"
                     /translation="MEISQKSFCKNLKTSIFRFYYLSLFLLLLLSGATEGFAQPVLSA
                     SFSNYNGYNISCNGFSDGQIDLTVNGSGPFNIQWSNGATTEDISGLTAGTYTVTVVDV
                     ANDTTIETYSLTEPDAITRSAVSIQDVTCFGLNNGSINISVAGGVPNYSFQWSNSSSN
                     EDISSLIAGVYTLTITDLNNCTSVFANDTVHEPDEIKITATITNTSCGLINGMIDASI
                     SGGISPYGYLWSNSNTTEDLNILSSGVYTLTVTDQNLCTKVDSFTVGASTQPSVVLDS
                     AFNVTCFGQSTGSIYVHVNDANGPVTYLWSNAATTQDIVNIAAGTYTVTVTDS"
BASE COUNT         1407 a          865 c          731 g         1555 t
ORIGIN      
        1 catcaggttg cttatttaat ccgaatttat ttattacctg tgtacaaaat tctgttagcg
       61 aataaagctt actatgcttt acatactttt caatacaagt ttcaattacc ttagtttcca
      121 tttgcagatg ttttgacaca aaatacaaaa tagacttctc tgctacctga ttttcattat
      181 cagcattgag tctaagacaa tttatcagaa acctgatttg cggtgcatgg caaatcaata
      241 gtgaatcgcg tgaattaata ttcttgtaac cattcaaaac taaaaagtct gccaacttgt
      301 ttccatttat attacttctg acaagcaatg caatatctcc aggcttatat cctttttcaa
      361 tctggctttg tatgtaactt agcgtatgtg atgcaatcac cttatcggca gcactaaaat
      421 attcaggata atgtaactca gaatacgatt catcttttgt gaatagttca atagatacaa
      481 aacccctttc atcactacgc tttgcaattt gatgtactgt attttcctga tatgctgcct
      541 tcagcaattg tgattgttgt gagtcagtag aaatgatttc acttattgcg ctaaaaaaaa
      601 tattattaaa ctcaactacg gatacagatg aacgaaaatt agaatctaac cgaaatacat
      661 ctgtactatt tgcatgattc tttagcgtgc tttccacttc attcaaaaga agattcatat
      721 ctcctccccg ccaacgataa attgattgct taccatcacc aacagtaaga acctgatgtt
      781 gttccgccaa actattaatt agcaatggca acaaattttt ccaatgcact tgtgacgtat
      841 cctgaaactc atcaatcaaa aaatttctgt acttgttgcc agccttttca taaataaatg
      901 gtgtgtcatt gtccccgata attgagttca atattttacc cacatcactc atgagcagca
      961 tctgatgatc cttgcgataa acttttaact gccgaaacag atatttaaga attacggcct
     1021 ggtaaatatt tgttgtaaca acttcagtag acaatagttg ttcaacgctt tctacataac
     1081 agtcaactat ttgccttact atgcccaaca attcgctatt caggttctga tcttgtttaa
     1141 gaaaatcatg agctttatct gcaaacacat ttttacgatc aagaatctgc catgtccgca
     1201 gattgatagt attatcttta ggatgttcct ttgcggctat tttttttatt aaagaataaa
     1261 atcctttttc accaaagtaa aaatcttttg actctgctcc tactgaattt gctatctcta
     1321 agaatttttt tgccattcct gacattttat tccagatagc tgcatgcaaa ccccttactt
     1381 cattaatcac cgatgtgtcg gcaagtacat ctgtttcaaa tttaactcta atgtgatcct
     1441 taaaaacttc tttagcaatg gtcttcagtt caccctgcat attccaaccc ttattgctat
     1501 ccactctgtg cattgcaaaa tcaataagcc aattggtaat atcaacatct gttccggcag
     1561 catgcaaaat attctgaacc ataaagtcta tggcaggttc tgtatttaaa tcaatttcat
     1621 atctgccagg cagtctcgat tcatatgcaa tggaacgggt gatgttcaga aaaaaactat
     1681 caatagtact aatggaaata tcagtataat gatgtatagc ataacttaaa acctttgctg
     1741 ctgcttgttc tatattaaag gatgttccaa gtgatgaagc taaatctgat ttcagttttt
     1801 cattccgacc ggtagccagt tgccataaag caaggatgag tctttctttc atctcaccgg
     1861 ttgcagcatt ggtaaacgta acggcaagcg ttccttttaa agcagcaggt gattttaaaa
     1921 caagcaacaa atactcttta acaagtgtgt atgttttacc cgaccctgct gacgattgat
     1981 atattttcag atttcccaaa atacttttta tgagttttca aatttaagaa tttgggatga
     2041 tttttatgat gtaatcaaaa tgattgaatt acattcaaaa ttaaatgtta tcaaatgaga
     2101 gaaaaaataa attgaatgat tagtaattta aaaacaacat caacgcaaat taaccggttg
     2161 aatcagctat aggaaacata attttcaaat gaataaaacc ggtattcaat aagaacaaat
     2221 tcaattctta ttctttaaag cctgacttaa taagtgtttg tgaataggca cgcaccattg
     2281 ttttatctaa cctgttttca agttcatatt ctttggcaac cgcttcgtta tggattctta
     2341 actcttcgcc caatgggaac aatgcattga ttgatgcacg caggctgttt cttttttcta
     2401 caaattcttt tgcaagcagc tccaaatcaa ttgaagattc attatttgac tttacagatt
     2461 gtctaactgc cgcaaggcgt gattgtggat attcttcttt gtattgcgtc acatcccaca
     2521 ttagcatttg tgaagtgtag tggtcgctga gtttgaattt taatcttgaa taaaaataag
     2581 cttcatagga acgcactgca tggtcatcag ctttaagcat aagtttcaga cgctcaactc
     2641 cggttttgcc gtaaggtata aaagttttat tcaatgaatc gtttaatgaa gctaatgcca
     2701 tcacatttac cttttccttg ataagtggtg ctctgttgtt tacaaaaacc tgaaacgctt
     2761 gtcctccggt cagctttgct atctcttccc atcccggcaa ttcacgctga cgcatttgtt
     2821 tgtcattttt aatacaataa actgtatgaa tttcaatatt atttttcact gctgtttcac
     2881 actgagccct gaaatcaaac cttccaaggt tgacataacc attgcccatt acaaacatca
     2941 ttttaacagc atcaggtttc tcactccagg acatatcgtt aactcctgaa cctatagctg
     3001 cacccacaaa ctgaattccg ttctctacat aaggttgtat gtcgaacatt ttttgtgaaa
     3061 gtgcatcata gtcatctgtt aaatcagaaa tgacttttat gtacccactg ccatcatgaa
     3121 aggaatatct tgaaaaacca atcaacccta ttctaatcac aggtttggga gtaaacatct
     3181 gccattgatt gataatatcc cacattttgt ctctaaaatt atcaagcaag ccgttggtac
     3241 taccgctaag gtccatacaa attaccacat caacctgacg aggctttgta cttgtattgt
     3301 taatttgttg tgattgacca tcagccgaaa cgaaaatcag cagtattgtt aaaatactaa
     3361 agctaatgat ggattttaaa gagttcattg tgtatgatgt tgttcctttt gtacggaaat
     3421 tatgcattaa agttatattc atttaaaaac taaaatcgaa gaaaaatcgg tagttattac
     3481 attgtttttt taccaaattc ataatcatat aagcggattt ttctattttt ggctcgagaa
     3541 ctttaaaatt catgcaccgc ttttacctat ttatggaaat aagccagaaa tctttttgta
     3601 aaaacctgaa aaccagcata ttcagatttt attatttaag tttattcctc ctgttattgt
     3661 tgtcgggtgc aactgagggc tttgcgcaac ctgtactttc tgcttcattt tcaaactata
     3721 acggttacaa catcagttgc aatggcttta gtgatggtca aattgatttg acagttaatg
     3781 gtagcggccc ttttaatatt caatggagca atggtgcaac aacagaagat attagcggat
     3841 taacagcagg aacttatact gtaactgttg ttgatgttgc caacgataca acaattgaaa
     3901 cttattctct tactgaacct gatgctatta caagaagtgc agtttcaatt caggacgtaa
     3961 catgttttgg gttaaataat ggaagcatta atatttcagt tgcaggcggt gtcccaaact
     4021 atagttttca atggtcaaat agcagctcga atgaagatat atcaagtttg attgccggag
     4081 tttatacttt gacaattaca gatttgaata attgtacttc agtttttgcc aatgataccg
     4141 ttcatgagcc tgatgaaata aaaattacag caacaattac gaatacttct tgtggattga
     4201 ttaacggaat gattgatgcc tcaatttctg gaggcatttc accttatgga tatttatggt
     4261 ccaattcaaa cacaacagaa gatcttaaca ttctttcatc aggcgtttat acactaacag
     4321 ttactgatca gaatttatgt acaaaggttg atagttttac tgttggtgca agtactcaac
     4381 cgtctgtagt gctcgactct gcatttaatg ttacatgttt tgggcaaagt actggaagta
     4441 tttatgttca tgtaaatgat gccaatggcc ctgttaccta tttgtggagt aatgctgcta
     4501 ccactcagga catcgttaac attgctgcag gaacttatac cgttactgtt actgattc
//
LOCUS       DAOQNP010000126         4511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_8390_length_4511_cov_5.110413, whole genome shotgun sequence.
ACCESSION   DAOQNP010000126 DAOQNP010000000
VERSION     DAOQNP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8390_length_4511_cov_5.110413"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(390..776)
                     /locus_tag="PKY09_12005"
     CDS             complement(390..776)
                     /locus_tag="PKY09_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13617.1"
                     /translation="MGITIFTPSSETKTMYENFSQPILPFRKFRNRLLKNFFWSCLIL
                     LFSLVMGMIGYHYLEGLSWIDSLLDAAMILTGMGPIHALTNDYAKIFATIYALYSGVA
                     FLTVIAVFLAPVIHRFMHHFHFDDDN"
     gene            863..2914
                     /locus_tag="PKY09_12010"
     CDS             863..2914
                     /locus_tag="PKY09_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB13618.1"
                     /translation="MRSFLLFLIGFVWTCCCTAQNNLSGMPEQDCFGAIPICQTTYST
                     TASYTGSGNIPNEINTAYSCLSSGEKNDVWYLFRTHSAGTISFTITPVNTADDYDWAV
                     FNITQKSCSNIYNDSSMEVSCNYDANIGCNGVTGANGNFGPCGQTENVITVLANESYL
                     INISNYSSSQSGYTIDFSASTALIFDVTPPHVVNDTLACTQTSLRVKFNEPVLCNTIT
                     TSGNDMILVDDSGNLQFPVSITFVGCDSAHPYITEAELFFAQPFAGSNNFYLLAANGS
                     DGNTISDLCGNFVNVSDTIGKFYSYNNMSVDLGSDLWTCSYDTLPVLYSASTPAANYN
                     WYYNNILLNQHLSYLQPTDTGQYILQAYLGAICQATDTIVINRKSTVTVSLGNDINIC
                     KESSFPEINCTANSSHLMWYLNNIPSLSDTSIFIPTDTGNVVVQAWSEGYCMAFDTLH
                     VGYFNMPLSLLPDSVARCVNSRIMLSSGINDAGTFLWIFNDDTLQNNQSWLDVSKEGF
                     CKMLFTTTDGCMVSDSTLVINSIEPDAPQLNCPILTAQGNKFTWQPYEGAVLFYISTD
                     GINYDSLPHDITSYIAASGINTVSLYVYDGSCNSKVAVTENCKPQISTLWDKDNGFVV
                     SGMSNPIILTVYDAQGREVYTTKDYRNNWSGQGLENGIYFFALKSAKAVYFYGKFLMV
                     R"
     gene            complement(2924..3355)
                     /locus_tag="PKY09_12015"
     CDS             complement(2924..3355)
                     /locus_tag="PKY09_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNB13619.1"
                     /translation="MIKSSKGSIAIIILLVLAVLTIKGIKSCRSTNDKTETPAVPQWK
                     DDGRNKRSEWRYQPLEYTAHAKCRMQCRQISEAEVSEILRSGTINYKKSNLNDKPCPT
                     YAIEGNTNDGQKVRIVFAACKTNTRVVTTIDIGKEHECDCK"
     gene            3428..>4511
                     /locus_tag="PKY09_12020"
     CDS             3428..>4511
                     /locus_tag="PKY09_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB13620.1"
                     /translation="MKETLVEKDFGIAQVLKQLGIAENNKGAATGGKWLTTKGKVIDS
                     YSPVDGQKIGSVQTATADDYAAVMQTAEEAFKVWRLMPAPKRGEIVRQIGDELRKYKE
                     PLGKLVSFEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLTMHSERPMHRMYEQWHP
                     LGVVGIISAFNFPVAVWSWNSMLAWVCGDVCIWKPSSKVPMCAIACQNIVSEVLKRNN
                     IPEGVSCLVIGNESGDLINNDKRIPLVSFTGSTRIGRHVSKTVAERFGRTILELGGNN
                     AIIVSENADLNMVLIGSVFGAVGTAGQRCTSTRRLIIHESVYNKTLDVIKNAYAQLKI
                     GNPLDASNHVGPLIDKGAVQDYLNAIE"
BASE COUNT         1401 a          810 c          900 g         1400 t
ORIGIN      
        1 gaactccgtt gagttccctg ttcgtaacct aaaactaacc ctatgaaaaa aatatcttta
       61 tgttactcgt ttctaaatta cagtgctaaa gtacaacatt tatattaaaa aacaatgaat
      121 tcaattattt tttcaccttt tgaaaggttt tgactaaaat tgactgtttc tatgttaact
      181 aagcacatat aagtataacc acgaattgct aaaattgttt taaccagatg caattttttt
      241 tactttcttc tcttataagg ctcaagacat taaaaatcaa cagctaaatt atgataaaca
      301 gcaatgaaaa caagtttgat ggatttaaca aatcattaag aaataaaaat agctgctaat
      361 tgtgtgctgt aagaaatgga ataaaaagtt taattgtcat catcgaaatg aaagtgatgc
      421 ataaaacgat gaattacagg tgcaagaaaa acagcaataa cagttaaaaa tgccacacca
      481 ctgtacaaag cgtaaatggt tgcaaaaatt ttagcatagt cgtttgtaag tgcgtgaata
      541 ggtcccatac ctgttagaat cattgcagca tcaagtaatg aatctatcca tgaaagacct
      601 tcaagatagt ggtaaccaat cattcccata accaatgaaa acaacagtat cagacatgac
      661 caaaagaagt tttttaaaag tctgtttcgg aatttcctga acggaagaat gggctgtgaa
      721 aaattttcgt acatagtttt tgtttcactc gagggagtaa aaatagttat tcccatgagc
      781 agaaagatta atttcaggtg taaggttttg tcagttatgt tctcaacccg gagaatgaaa
      841 aatcgtatca ttaataattc ggatgagaag ttttttatta ttccttattg gttttgtttg
      901 gacttgctgc tgtacggcac aaaacaactt atcgggtatg cctgagcagg attgttttgg
      961 agctattcct atttgtcaga caacctattc tactacggca tcatatacgg gatcgggaaa
     1021 tattcccaat gaaatcaata ctgcctattc ctgtctttca tctggcgaaa aaaatgatgt
     1081 ctggtatttg ttcagaacac attctgctgg aacaatcagt tttactatca ctcctgtaaa
     1141 tactgctgat gattatgact gggcagtgtt taacatcaca caaaaatctt gcagcaacat
     1201 ttacaacgat tcttcaatgg aagtaagttg taactatgat gccaatatag gatgtaatgg
     1261 tgttacaggg gctaatggaa attttggtcc ttgtggtcag acagaaaatg tcattacggt
     1321 tcttgcaaat gaatcatatc tcatcaacat aagcaactac tcctcatcac aaagcggcta
     1381 cactattgac ttctccgcat caacagcatt aatttttgat gttacaccac cacatgtggt
     1441 taacgataca ttggcttgta cgcaaacatc tttaagagta aaatttaatg agcctgtgct
     1501 ttgcaatacc attaccacat ccggtaacga tatgattctt gttgacgaca gtggaaattt
     1561 acaattccct gtaagtataa cttttgtggg ttgcgattct gcacatcctt acattacgga
     1621 agccgagtta ttttttgctc agccttttgc cggaagcaat aacttttatc tgcttgccgc
     1681 aaatggcagt gatggcaata ccatttccga cttatgtggc aactttgtga atgttagtga
     1741 tacaattgga aagttttaca gttataataa catgtctgtt gatttaggaa gcgatttgtg
     1801 gacatgcagc tatgatacat tacctgtttt gtattcggct tcaacaccgg cagcaaatta
     1861 caactggtac tacaataata ttctgttgaa tcaacacctt tcgtatttgc aaccaacaga
     1921 taccggacaa tatattttac aggcatactt aggagcaatt tgtcaagcaa cagacaccat
     1981 tgttataaac cgaaaatcca cagtaacagt aagtctgggt aatgatatca atatttgcaa
     2041 agagtcttct ttcccggaaa taaattgtac tgccaacagt tcgcacttga tgtggtattt
     2101 aaataatata ccttctttat ccgataccag tatttttata ccaaccgaca ccggtaatgt
     2161 tgttgtgcag gcatggtcag aaggatattg tatggcgttt gatacactac atgttggtta
     2221 tttcaacatg cctttatcac tattgcccga cagtgttgca cgatgtgtaa acagccggat
     2281 aatgttgtcc agcggaatca atgatgctgg cactttcctc tggatattta atgacgatac
     2341 acttcagaat aatcaatcat ggcttgatgt ttcaaaagaa ggattttgca aaatgttgtt
     2401 tacaacaact gatggttgta tggtttctga ttcaacgctt gtcatcaata gcatagaacc
     2461 tgatgcacca caattaaact gtcctatact cactgcacaa ggtaataaat ttacttggca
     2521 gccatacgaa ggtgcagtat tattttatat tagtactgat ggaataaact acgacagttt
     2581 accgcacgac attacctcat atattgcagc atcaggcata aatactgtct cactttatgt
     2641 ttatgacggt agttgtaatt caaaagttgc agtgacagaa aattgtaaac ctcagatttc
     2701 aactttatgg gataaagaca atggctttgt agtttccggg atgagcaatc caataatttt
     2761 aactgtttat gatgcacaag gccgtgaagt ttatactaca aaagattatc gcaacaactg
     2821 gtcaggtcaa ggtcttgaaa atggtattta tttctttgcg ctcaaatcgg caaaagcagt
     2881 ttatttctac ggaaaatttt taatggtgcg ataaggagga tacttatttg caatcacatt
     2941 catgttcctt gccaatgtca atagttgtga ctacgcgtgt atttgttttg caggctgcaa
     3001 aaacaatgcg cactttctgg ccatcgtttg tattcccttc aatggcataa gtagggcatg
     3061 gtttatcgtt cagattactt tttttgtagt tgatggttcc gcttcttagt atttcagaaa
     3121 cctctgcttc gctgatttgt cggcattgca tacgacactt ggcatgtgca gtgtattcca
     3181 aaggctgata gcgccattcg ctgcgtttgt tgcggccatc atctttccat tgtggcacag
     3241 caggcgtttc tgttttatca ttggtggaac ggcaactttt tatgcctttg attgttaata
     3301 cagcaagcac taacaatata atgatggcaa tagaaccttt agaagattta atcataaaca
     3361 aggcattgaa ttgcttaatt ttgcaggcta aaattacatt tcaaaagcaa ataaaaaaca
     3421 gttttatatg aaagaaacat tggttgaaaa agattttggc attgcacagg tgctaaaaca
     3481 attaggtatt gcagaaaaca acaagggcgc agccacaggt ggtaaatggc tgaccaccaa
     3541 agggaaggtg attgattcct actcaccggt tgatggtcag aagataggca gtgtgcaaac
     3601 agctactgct gatgattatg ctgccgtaat gcaaactgcc gaagaggcat ttaaagtatg
     3661 gcgattaatg cctgcaccta agcgtggaga aatagtgcga cagattggtg atgaacttcg
     3721 taagtacaaa gagcctttag gaaaattggt ttcttttgaa atgggtaaaa gccttcagga
     3781 aggactgggc gaagtacagg aaatgattga tatctgcgat tttgccgtgg gtttatcacg
     3841 tcagctttat ggacttacca tgcacagcga gcgacccatg caccgtatgt acgaacaatg
     3901 gcatcccttg ggtgtggtag gtataatctc agcctttaac tttcctgtgg ctgtatggag
     3961 ctggaactcc atgctggcat gggtttgtgg tgatgtgtgt atttggaaac ccagctcaaa
     4021 agttcctatg tgtgccattg cctgccagaa tattgtgtca gaagttttga aaagaaacaa
     4081 cattccggag ggggtaagct gcttggtgat aggtaatgaa tcgggcgact taatcaataa
     4141 tgacaaacgc ataccattag tttcttttac aggttccaca cgcattgggc gacatgtttc
     4201 taaaactgtt gccgaacgtt ttggacgtac cattcttgaa ctgggaggaa acaatgcaat
     4261 tatagtttct gaaaatgcag atttgaatat ggttcttata ggttctgttt ttggtgctgt
     4321 gggtactgca gggcagcgtt gcacttcaac acgaagattg attattcacg aaagcgttta
     4381 caacaaaacc cttgatgtga taaagaatgc ttatgcacaa ttaaagattg gcaacccatt
     4441 agatgcatcc aaccacgtag gcccattgat tgataaaggt gctgtacagg attatctgaa
     4501 tgccattgaa a
//
LOCUS       DAOQNP010000127         4506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_8401_length_4506_cov_7.168726, whole genome shotgun sequence.
ACCESSION   DAOQNP010000127 DAOQNP010000000
VERSION     DAOQNP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8401_length_4506_cov_7.168726"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PKY09_12025"
     CDS             complement(<1..1084)
                     /locus_tag="PKY09_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNB13621.1"
                     /translation="MKDTVVEKDYGIAQVLKQLGIAQINKGAATGSKWITTKGKTIDS
                     YSPVDGQKIGSVDAATADDYATVMKTAEEAFKVWRLMPAPKRGEIVRQMGDELRKYKE
                     PLGKLVSFEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLTMHSERPMHRMYEQWHP
                     LGVVGIISAFNFPVAVWSWNSMLAWVCGDVCIWKPSSKVPMCAIACQNIVSEVLKRNN
                     IPEGVSCLVIGNESGDLINNDKRIPLVSFTGSTRIGRHVSKTVAERFGRTILELGGNN
                     AIIVSEQADLNMVLIGSVFGAVGTAGQRCTSTRRLIIHESVYNKTLDVIKNAYAQLKI
                     GNPLDASNHVGPLIDKGAVQDYLNAIE"
     gene            1156..1587
                     /locus_tag="PKY09_12030"
     CDS             1156..1587
                     /locus_tag="PKY09_12030"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNB13622.1"
                     /translation="MVKTTSKASIAIIILLALALLTIKGIKSCRSTNENTEVAAPQWK
                     DDGRNKRSEWRYQPLEYTSHAKCRMQCRQISEAEVSEILRSGTINYKKSNLNDKPCPT
                     YAIEGNTNDGQKVRIVFAACKTNTRVVTTIDIGKEHECDCK"
     gene            complement(1597..3648)
                     /locus_tag="PKY09_12035"
     CDS             complement(1597..3648)
                     /locus_tag="PKY09_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNB13623.1"
                     /translation="MRHFLLHLIGFFWICSSHAQTNLSGMPEQDCFGAIPICQTTYST
                     TASYTGSGNIPNEINNAYSCLSSGEKNDVWYLFRTHSAGTISFSITPVNVADDYDWAV
                     FNITQKSCSNIYNDSSMEVSCNYDANIGCNGVTGANGNFGPCGQTESVISVLANESYL
                     INISNYSSSQSGYTIDFSASTALIFDVTPPHASNDTIACTQTSLKVKFNEPVLCSTLT
                     SSGSDMILADDNGNLQIPVSITFVGCDSAHPYITEAELFFAQPFAGSNNFYLLAANGS
                     DGNTISDLCGNFVSVSDTIAKFYSYNNMTVDLGSDLWTCSYDTLPVLYSASTPVANYN
                     WYFNNTLLNQHFSYLQPADTGQYILQAYLGSFCQATDTIYIIRKQAVSVSLGTDINIC
                     KESSFPEINCTTNSSHVMWYLNNIPYASDTNIFTPADTGSVVVQAWSEGYCMAFDTLH
                     VGFYNVPLSLLSDTVARCVNSQIELLSGINDNGTFLWIFDGDTLQNNQSWLDVTKEGF
                     CKMLFTTTDGCTVADSTHVINNPEPEAPQLNCPILTANGNKFTWQPYQGAVRYYISTN
                     GENYNSLPQDITSFIAALGVNAVSLYVYDGSCNSKVAVSENCKPQISTLWDKDDGFVV
                     TGLSNPISLTVYDVQGREVYTTKDYRNNWSGQGLENGIYLYTLKSAKAVYFYGKFLMV
                     R"
     gene            3736..4122
                     /locus_tag="PKY09_12040"
     CDS             3736..4122
                     /locus_tag="PKY09_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13624.1"
                     /translation="MKIATFTPSIQTTNMYENYSQPILPFKKFSLRLFKNFLWSCSIL
                     LLSLLAGMVGYHYLEGLSWIDSMLEASMILTGMGPLHPLTNDSAKIFATIYALYSGVA
                     FLTVIAVFLAPVIHRFMHHFHFDDDN"
BASE COUNT         1412 a          911 c          805 g         1378 t
ORIGIN      
        1 tttcaatggc attcagataa tcctgtacag cacctttatc aatcaatggg cctacatgat
       61 tgcttgcgtc taaggggttg ccgattttta attgagcgta ggcattttta ataacatcaa
      121 gggttttgtt gtaaacgctt tcatgaataa tcaatctgcg tgttgaggtg caacgctgac
      181 cggcagtgcc cacagcacca aaaacagaac cgatgagtac catattcaaa tcggcttgtt
      241 cagaaactat tattgcattg ttgccgccta attcaagaat ggtgcgtcca aagcgttctg
      301 ctactgtttt agatacatgt cgccctatgc gtgttgaacc ggtaaaagaa actaaaggaa
      361 tacgtttgtc attattgatt aagtcacccg attcattgcc aataaccaga cagcttaccc
      421 cctcaggaat gttgtttctt ttcaaaactt ctgaaacaat attctgacat gcaatggcac
      481 acattggcac tttggaactt ggtttccaaa tacaaacatc gccacacacc catgccagca
      541 tggagttcca actccaaaca gcgacaggaa aattaaatgc agaaataatg cctacaacac
      601 ccagtggatg ccactgctca tacatgcggt gcatgggtcg ctcgctgtgc atggtgagtc
      661 cgtacaactg acgtgataag cccacagcaa aatcgcagat gtcaatcatt tcctgcactt
      721 cacccagccc ttcctgaaga ctcttgccca tttcaaaaga aactaatttt cctaaaggct
      781 ctttatattt acgtagttca tcacccatct gacgtacaat ttcgccacgc ttgggtgccg
      841 gcattaaacg ccatacctta aaagcttctt cagcagtttt catcactgta gcataatcat
      901 ctgcagtagc ggcatccaca ctgcctattt tttgcccgtc aacaggtgaa taagagtcta
      961 tggttttacc ttttgtggta atccatttac tgcctgtggc agcaccttta tttatttgtg
     1021 ctattcccaa ctgtttcagc acttgtgcaa tgccataatc tttctcaact actgtgtctt
     1081 tcatatttgt ttatttttat ttgctgatgt aatgtaattt tagcctgcaa aattaagcat
     1141 ttcaacgcct tgtctatggt aaaaacaaca tctaaagcat caattgcaat aattatttta
     1201 ttggcacttg cattactaac aataaaaggc attaaaagtt gccgttcaac gaatgaaaat
     1261 actgaagttg ctgcacctca atggaaagat gatggccgca ataaaagaag cgagtggcgc
     1321 tatcagcctt tagaatacac atcacatgca aagtgtcgca tgcaatgtcg ccaaatcagc
     1381 gaagcagagg tttctgaaat cttaagaagc ggaaccatta actacaaaaa aagtaatctt
     1441 aatgacaagc catgccccac ctatgccatt gaaggaaata caaatgacgg acaaaaagtg
     1501 cgcattgttt ttgcagcctg caaaacaaat acacgtgtgg taacaacgat tgacattggc
     1561 aaagagcatg agtgtgattg caaatagatg ttcaccttat cgcaccatta aaaattttcc
     1621 atagaaataa accgcttttg ccgatttgag ggtatataaa taaataccgt tttcaagacc
     1681 ttgccctgac caattgttgc gatagtcttt tgtagtatag acttcacgac cctgtacatc
     1741 ataaacagtt aaacttatgg gattactaag acccgtaact acaaaaccgt catccttatc
     1801 ccataatgtt gaaatctgag gtttgcaatt ttcagataca gcaacttttg aattgcagct
     1861 accgtcataa acataaagag aaacggcatt tactcccaat gctgcgataa aagaggtaat
     1921 gtcctgtggc aagctgttgt agttttctcc atttgtactg atataatatc ttaccgcacc
     1981 ttggtaaggc tgccatgtaa atttatttcc atttgctgta agtatagggc agtttaattg
     2041 tggtgcttcc ggttcagggt tattgatgac atgagttgaa tcagcaaccg tacaaccatc
     2101 agttgttgta aacaacattt tgcaaaaacc ttctttagta acatctaacc acgattgatt
     2161 attctgaagt gtatcaccat caaatatcca gagaaaagtg ccgttatcat tgataccact
     2221 caacaattct atctgactgt ttacacatcg tgcaacagta tctgacaata gagataaagg
     2281 cacattgtaa aaaccaacat gtaaggtatc aaatgccata caatatcctt ctgaccatgc
     2341 ctgcaccaca acacttccgg tgtcggccgg agtaaagata ttggtatcgg atgcataagg
     2401 aatgttgttt aaataccaca ttacgtgtga actgttggta gtacaattta tttcagggaa
     2461 agaagactct ttacaaatat taatatcagt acccagactt acagatactg cctgtttacg
     2521 gattatataa atggtgtctg ttgcttgaca aaatgatcct aagtatgcct gtaaaatata
     2581 ctgtccggtg tcagcaggtt gcaaataaga aaaatgttga tttagcagcg tattgttgaa
     2641 ataccaattg taatttgcaa cgggtgttga agcagaatat aaaacaggta acgtatcata
     2701 gctgcatgtc cacaaatcgc tgcctaaatc aacagtcata ttgttgtagc tgtaaaattt
     2761 tgcaattgta tcactaacac tcacaaagtt gccacataag tcggaaatag tattgccatc
     2821 gctgccattg gcggcaagca aataaaagtt gttgcttccg gcaaatggct gagcaaaaaa
     2881 taactctgct tctgttatgt aaggatgtgc agaatcacag cctacaaaag ttatacttac
     2941 aggtatttgt aagttaccat tatcatcagc aagaatcata tcactaccac ttgatgttag
     3001 tgtactgcaa agaacaggtt cattaaactt tacttttaaa gatgtttgtg tacaagctat
     3061 ggtgtcgttg gatgcatgtg gcggtgtaac atcaaaaatt aatgccgtgg atgcagaaaa
     3121 gtcaatggta tagccacttt gtgaggagga atagttactt atgttgataa ggtatgattc
     3181 atttgcaaga acagagatga cactttctgt ctgaccacaa ggaccaaaat ttccattggc
     3241 acccgtgaca ccattacatc caatattggc atcataatta cagctgactt ccattgaaga
     3301 atcgttgtaa atattgctgc atgacttttg tgtgatgtta aatactgccc aatcataatc
     3361 atcagccacg ttaaccggtg taatgctgaa actgattgtt ccggcagaat gtgttctgaa
     3421 caaataccaa acatcatttt tttcgcctga agaaagacac gaatatgcgt tgttgatctc
     3481 attgggaata tttcctgacc ccgtatatga tgccgtagta gaataggttg tctgacaaat
     3541 aggaatagca ccaaaacaat cttgctcggg catacccgat aaattagttt gtgcatgact
     3601 gctgcaaatc cagaaaaaac caataaggtg aagtaaaaaa tgtctcatcc gtatttagta
     3661 tgatacgaat ttctattcac catgttgaat acatatcccg aaaaatctta cactataaat
     3721 taatcttctg cactattgaa aatagctact tttactccat ctattcaaac aacaaacatg
     3781 tacgaaaact attcgcaacc catactccct tttaaaaaat tcagtctccg gctgtttaaa
     3841 aactttcttt ggtcgtgtag catactgtta ctttctctcc ttgccggaat ggtcggatat
     3901 cactatcttg aaggattatc atggatagat tctatgctgg aggcatctat gattcttacc
     3961 ggaatgggac cactacaccc cttaaccaac gactctgcaa aaatctttgc aactatttat
     4021 gccctttata gtggggtagc gtttttaacg gttattgcgg tttttctggc accggtcatt
     4081 caccgtttta tgcatcattt tcattttgat gacgataatt aaactttttt ctctgttctc
     4141 ttacaagtca ataaaatgcc gcagactatt agttaaatgc tatgttactt aaaaattcta
     4201 taccaacagc agcctcatgc acaaaaccga acacttgatt tataatagct tatgcctgac
     4261 attaaaataa ttttacccta tcataaaact tttttattgt atggatgttc ttaatagtaa
     4321 tgagtttggt tcttttgaaa gattaaaaat tatccaaaat cttttcaaaa catgaaattt
     4381 ctactgaatt cattgttttt taatattaat gttgtacttt agcactgtaa tttagaaacg
     4441 agaaacacaa aatatttttt tcatagggtt agttttaggt tacgaacagg gaactcaacg
     4501 gagttc
//
LOCUS       DAOQNP010000128         4489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_8451_length_4489_cov_7.462336, whole genome shotgun sequence.
ACCESSION   DAOQNP010000128 DAOQNP010000000
VERSION     DAOQNP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8451_length_4489_cov_7.462336"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(492..1616)
                     /gene="carA"
                     /locus_tag="PKY09_12045"
     CDS             complement(492..1616)
                     /gene="carA"
                     /locus_tag="PKY09_12045"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016195484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNB13625.1"
                     /translation="MKYAHKSPAVLLLQDGTVFYGKSAGKIGTATGEICFNTGMTGYQ
                     EIFTDPSYKSQIMVMTSVHIGNYGVCQAESESSSAKISALVCRNFSDHFSRKKADQSL
                     QDYFIENNMVVVSDVDTRAIVQHIRSKGAMNAIVSSEETDLVKLKAMLAKVPSMEGLE
                     LSSTVTTNKAYTYGDPVAALHVAVIDLGVKENTLRCLADRGCYVKVFPMNTSFEEMEK
                     FNPQGYLVSNGPGDPAVMPAMVDTVKKILNSDKALMGICLGHQMLAEACGIKTFKMFN
                     GHRGINHPVKNMVTGRAEVTSQNHGFGVIPEETINKAEIEITHVNLNDQSIEGISVKN
                     KKAFSVQYHPEAAPGPNDARYLFDNFVSSVREVKEQAVTV"
     gene            complement(1689..2378)
                     /gene="radC"
                     /locus_tag="PKY09_12050"
     CDS             complement(1689..2378)
                     /gene="radC"
                     /locus_tag="PKY09_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNB13626.1"
                     /translation="MEEPKNIKSWSESDRPREKLLEKGKEALTDSELIAILIRSGSRQ
                     ESAVDLAKRILKNAHNELSELSKQSVKDMMRYKGMGKVKAITIVAALELGRRRAQAQA
                     LERQKITSSKDAVAYFKALLADLPHEEFRILLLDRANQIIKSENISKGGVSGTVVDAR
                     IIFKSAIENLASGIILCHNHPSGNPKPSQADVNLTSKLSQAGKLLEINILDHVIIAGD
                     DYYSFADEGQL"
     gene            complement(2452..2706)
                     /gene="rpsT"
                     /locus_tag="PKY09_12055"
     CDS             complement(2452..2706)
                     /gene="rpsT"
                     /locus_tag="PKY09_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNB13627.1"
                     /translation="MANHKSAVKRIRANGAKKDRNRYQAKTSRNALKELRTTTDKKEA
                     EKLLPEVSGMLDKMAKKNLIHKNKAANLKSSITKYVGALK"
     gene            complement(2758..2829)
                     /locus_tag="PKY09_12060"
     tRNA            complement(2758..2829)
                     /locus_tag="PKY09_12060"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2794..2796),aa:Glu,seq:ttc)
     gene            complement(3053..>4489)
                     /locus_tag="PKY09_12065"
     CDS             complement(3053..>4489)
                     /locus_tag="PKY09_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNB13628.1"
                     /translation="DVASRWGRLNFNIRHRFKNIEGLSAGINGNFMQSHSNFSLVWAN
                     DSTGIYNAFPKTMTLTDSKAFYIDPYISLTGKKGSSHQLRSRIFYADNDNGNDQSNKT
                     NVYLAEYRFNRELISLGRLNVTSGVYFQQSFSHASLYAGSGNDKNTLENYAAFIQLEK
                     KLIKKLMVSAGFRAEYFEMNNKESQLEPILRAGLNYQLTEGTYIRCSYGQGYRYPTIA
                     EKFIETGAGGISVFPNPEVQPESSWNAEAGIKQGFKIGNFFGFADIAVFEQRYHNTIE
                     YIYALWQPDQLAGFKFVNTGNSRVKGLEVSIMGEGKPVRNFAISFLGGYTYTLPQSLE
                     PDYIFATDNPPTDFLPDSLSYSSTSTDTSKNILKYRFQHIAKMDIEFTWKFISFGYGV
                     RYYSFMKNIDKKFYDLDETVLPTGVKAYREKNDKGTLVHDLRLGFKLSEIFKASFLVN
                     NATNLEYSLRPLKIESPRNYTIQLTAAF"
BASE COUNT         1286 a          875 c          846 g         1482 t
ORIGIN      
        1 aggcaatatt tactgtttgt gattgcgccc ttgtaaagaa tgtaaacatg cagagtgcaa
       61 tggctattgc agtagtttgg attaggtttt tcatcatgtt tggttttaaa gggtttgact
      121 aaagtacagt gattattaga atttcgtatg cagtaccaaa aaaattgcgc ggtgcttagt
      181 catttagcct gaggtagccg cgaagcaccc accgaacaaa taaacaaagc ccgcgcttta
      241 cgcgagcgtt tgcttattct tgttcagttt aaatttcgcg gattttcaga ctaagaattt
      301 gctaacgcaa tattttatgt atgctttttt tatgtcattt ttttgaatta aattgtgtta
      361 caatggtaca attattttta gaaaaggcaa aatatttttg agggaaaaaa gaaaagggtt
      421 tttgttgtga tattaaaaac ctcttgaaat taaaatcaaa aagaaacaaa attacaatat
      481 catagggtag cctataccgt aactgcctgc tcttttactt ccctaacgga acttacgaag
      541 ttgtcgaaaa gataccgtgc atcattaggg ccgggtgcag cttcggggtg atattgtaca
      601 gaaaaagctt ttttattttt tacgcttata ccctcaatac tctgatcgtt caggtttacg
      661 tgtgtaattt caatctctgc tttattaatg gtttcttccg gaataacacc aaagccatga
      721 ttttgtgatg tcacttctgc acggcctgtc accatatttt ttacagggtg attgatgcca
      781 cgatgtccgt taaacatttt aaacgttttt attccacagg cttctgcaag catctgatga
      841 cctaagcaga ttcccatcag cgctttgtcg gaattcagaa tttttttgac tgtatctacc
      901 atggcaggca ttacagcagg atcacccggg ccattggaaa ctaaatagcc ttgcggatta
      961 aatttttcca tctcttcaaa cgaagtgttc ataggaaata cttttacgta gcatcctctg
     1021 tcagcaagac agcgtaatgt gttttctttt acgcccaaat ctattacagc tacatgcaat
     1081 gctgctacag ggtcaccata agtatatgct ttattagtag taactgtgct tgataattcc
     1141 agtccttcca ttgaaggtac ttttgccaac attgctttca attttacaag gtctgtttct
     1201 tcagatgaaa caatggcatt catggcacct ttgctgcgta tgtgctgtac gatggcacgt
     1261 gtatcaacgt cagacacaac aaccatatta ttttcaataa aataatcctg cagactttga
     1321 tctgcttttt tgcgtgaaaa atggtcagag aaattgcgac aaaccaaagc agaaattttt
     1381 gctgacgaag attctgactc tgcctggcac acaccataat ttccgatgtg aacagatgtc
     1441 atcaccataa tctgtgattt ataacttggg tcggtaaata tttcctgata gcctgtcatg
     1501 ccggtgttaa aacagatttc accggtggca gttccaattt tgcctgccga ctttccataa
     1561 aataccgtac cgtcctgaag caacaataca gcgggtgatt tgtgtgcgta tttcatttcg
     1621 aaaagtttcg caaaagtacc attaaatcgg ttatcagatt ttttacttgt aaacaggctg
     1681 ttgaaaaatc atagctgccc ttcatcggca aaactgtaat agtcgtctcc ggcaattata
     1741 acgtgatcta agatgtttat ttccagtaat tttcctgcct gactgagttt tgatgtcaga
     1801 ttaacatcag cctgacttgg cttaggattg ccactgggat ggttatgaca aagtataatt
     1861 cccgaagcta aattttctat tgccgattta aatatgattc gtgcatcaac cacagttccg
     1921 gaaacaccac ctttgctgat gttttcggat ttgataatct gattggcccg atctaataaa
     1981 agtattctga attcttcatg cggaaggtct gccagcaagg ctttaaaata agccacagca
     2041 tctttacttg aagttatctt ttgtctttcc agggcttgtg cctgtgcccg tctgcgtcct
     2101 aactccagcg cagctactat agtgatagcc tttactttgc ccatgccttt gtacctcatc
     2161 atgtctttta ccgattgctt ggaaagttca gacagctcat tatgcgcatt ttttaaaata
     2221 cgtttagcca ggtcaaccgc agactcctga cggcttcctg aacggatgag aatagctatc
     2281 aattcactgt cggtcaaggc ttccttacct ttttcaagga gcttttctcg tggcctgtcg
     2341 ctttccgacc atgatttgat gtttttaggt tcttccatgg tgctaagata aggtgttaaa
     2401 caaaaaaagc ccgcattttt agcgagctta aaattcaaaa ttatgtgata attattttaa
     2461 agcacccacg tatttagtga tacttgattt caggtttgca gccttatttt tatgaataag
     2521 gttctttttt gccattttat caagcatacc ggatacttca ggcagcagct tttcagcttc
     2581 ctttttatct gtggtagtac gcagctcttt caaagcatta cgcgaagttt ttgcctgata
     2641 gcggtttctg tcttttttag caccgttggc gcgaattctt tttactgctg acttgtgatt
     2701 tgccataatt acagttttta cttattaatg atggatatta atccttaatt tttttagtag
     2761 cccgtacgag aatcgaactc gtgtttcctc cgtgaaaggg agatgtccta acccctagac
     2821 gaacgggcca aacgtttttt tgaggctgca aatgtataaa aacaatttac attcaaagcc
     2881 ttataaggca aatatttttc atttttctgc catttggctg aagtattcag tgttcttgat
     2941 aaaaaatcat ctttaacagc ttaagtaaga ctgaattaaa ggctgtgaac cgccaaagtc
     3001 taaccgtcta aaataaacag ataaacagct ttttaacaag tagaagtctt gattaaaagg
     3061 ccgctgtaag ttgaatggta taatttcttg gcgactcaat ttttaatgga cgcaaagagt
     3121 actcaaggtt tgtggcattg tttactaaaa atgaggcctt aaaaatttca ctgagtttga
     3181 aacctaaacg aaggtcatgc accaaggtgc ctttgtcatt tttttcacga taggctttca
     3241 cacctgtcgg taaaacagtt tcgtcaagat cataaaactt cttatcaatg tttttcataa
     3301 aactgtaata ccggacacca taaccaaaag aaataaattt ccatgtaaac tcaatatcca
     3361 ttttggcaat gtgctgaaaa cggtatttaa gaatattctt tgatgtgtct gtacttgtgc
     3421 tgctgtacga taatgagtcg ggtaaaaaat cagtaggagg gttgtctgtt gcaaagatgt
     3481 agtccggttc cagactttgc ggtagcgtat aagtatatcc acccaaaaaa ctaattgcga
     3541 aattgcgaac cggttttcct tcgcccataa tagaaacttc aagaccttta acgcgtgagt
     3601 tgcctgtatt cacaaattta aatccggcta attgatcggg ttgccataat gcataaatat
     3661 attcaatggt gttgtgatag cgttgttcaa aaacagcaat atcagcaaaa ccaaagaaat
     3721 ttccaatctt aaaaccctgc ttaattcctg cttctgcatt ccagcttgac tcaggttgta
     3781 cttcaggatt cgggaaaaca gaaattccac cggcacctgt ttctataaat ttttctgcaa
     3841 tggttggata acgatagcct tgtccgtaag agcaacgaat ataagttcct tctgtaagct
     3901 gataatttaa tcctgcacgt aagattggtt ctaactgcga ctctttattg ttcatctcaa
     3961 agtattctgc tctgaaacct gcagatacca taagtttttt tattagcttt ttttctaatt
     4021 gaataaaggc tgcatagttt tcaagcgtgt ttttatcatt gccactgcca gcatacagcg
     4081 aggcatgact gaaagattgt tgaaaataaa ctcctgaggt tacatttaat cttcctaaag
     4141 aaatcaactc gcggttaaaa cgatattctg ccagataaac attggtttta tttgactgat
     4201 cgttgccatt atcattgtcg gcataaaaaa tgcgcgaacg cagttgatgt gatgaacctt
     4261 tcttacctgt caggctgatg tatgggtcaa tataaaatgc tttagaatcg gtaagcgtca
     4321 ttgtttttgg gaatgcattg taaatccctg tgctgtcatt tgcccaaacc aaagaaaaat
     4381 tagagtgcga ctgcataaaa tttccgttga tacctgcaga aagcccttca atatttttga
     4441 aacgatgacg tatgttaaag tttaacctgc cccaacggga tgcaacatc
//
LOCUS       DAOQNP010000129         4463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_8531_length_4463_cov_8.983666, whole genome shotgun sequence.
ACCESSION   DAOQNP010000129 DAOQNP010000000
VERSION     DAOQNP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8531_length_4463_cov_8.983666"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PKY09_12070"
     CDS             <1..1230
                     /locus_tag="PKY09_12070"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13629.1"
                     /translation="FTLNLTGTGIKTQNIDATVQGLLKLNDRELATQGNVMHVTNTAP
                     AAITRTGGLNSTPVQGFVSSTGSGKLWRNTNATSVYLFPVGSSIPPARFRPVEIKPLS
                     ATPNTFAVRMVNNDPNTDGFNRAVREPTLGAVNAGWYQKINRVSGSSLAEFSLYADDA
                     QDAIASWPLLKLCQWGQAAPPIWWKDIGGAVTPGASPVLGSVLRTNWNNFSNTENFNI
                     APESNPLPVELLSFTGSCKNNIVNLNWTTASEINNHYFELQQSEDGSNFVSIGRVEGN
                     GTTIKQKFYSFTIDQKTERIFFRLVQYDFDGRANVLPAILVSCNTENPTTVNINLMPN
                     PAKDVFVSDIYLPEKTELSMKVFNTIGQLVLEEQWTLDAGYHKQATQVSTLAKDIYNI
                     RYTSNTFNIVKQIAIVK"
     gene            1237..1758
                     /locus_tag="PKY09_12075"
     CDS             1237..1758
                     /locus_tag="PKY09_12075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNB13630.1"
                     /translation="MAEIALRSLQIEDAELFALHANNNAIANNMTNQFPFPYTKAHAL
                     EFIRRVNGLKPAQVFAIDLDGEAIGAIGLHPQTDIFLKNAELGYWLAEKYWGKGFVSI
                     AIKKIVEYGFSNFEIDRIFARPFGSNKASQRVLEKTGFILEARLEKIIFKNNRYEDEL
                     IYAIRKSHNHPKT"
     gene            complement(1776..2660)
                     /locus_tag="PKY09_12080"
     CDS             complement(1776..2660)
                     /locus_tag="PKY09_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13631.1"
                     /translation="MENQSKGNNRIWIVLFAASALFNVYQWRNHTSTTEVFEVTKDSL
                     ITARVDVEKELGATIEELNKYKGINDNLDSLLTEANHKIDEQKERIQKLIRSEGNSAA
                     LNKKLMAELESLKKLRDEYLDKIDTLMVENQKLKDDNAQLSGTVETLSKNLETTVNTA
                     SVLKSEYFKVSTFKKKGNGKFVSTALSKRTNKMDVCFSVLENKIAKAGEKNVYLRIIE
                     PGGKVMGNRSAGSASFKMASGEELMCTVVSSLRYENATVDNCLSYEENDRIFTPGTYL
                     IEVYIDGNLSGASSYVLR"
     gene            complement(2769..3797)
                     /gene="tsaD"
                     /locus_tag="PKY09_12085"
     CDS             complement(2769..3797)
                     /gene="tsaD"
                     /locus_tag="PKY09_12085"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNB13632.1"
                     /translation="MDKKDIVILGIESSCDETSAAVSRNTYILSNVIANQTVHQKYGG
                     VVPELASRAHQSNIVPVVEQALQEAAIHLNDVDAIAFTRGPGLIGSLMVGVSFAKSLG
                     AALNKPLIEVNHMQAHVLANFLSIEGQIGRTPSFPFLCLTVSGGHTQIVKVTSSHQME
                     IIGQTLDDAAGEAFDKIAKVLELPYPGGPLIDKYALSGNPKAFHFPVANLPGLNYSFS
                     GLKTSVLYFLKKQLVQNPAFITQNRDDLCASVQYTIVKTLLLKLKKAAIETGINNIAI
                     AGGVSANSGLRKAITEEAVKSNWQVHIPPLSLCTDNGGMICLAAYFKYLAADFSTLDV
                     APSARFGF"
     gene            4083..>4463
                     /locus_tag="PKY09_12090"
     CDS             4083..>4463
                     /locus_tag="PKY09_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13633.1"
                     /translation="MRFQKVQSWVVDRVTGYLSSELNTTVHIGGVRFVLIKSVYLDDV
                     FIADKQQDTLLYAKHLTATVAFQSLFIQNKKLILDEVQLDKARIALSKSKSTRDMNFD
                     FIVDYFSSPSKDTTTSPFYFAVNRV"
BASE COUNT         1372 a          858 c          861 g         1372 t
ORIGIN      
        1 tttacattaa atctaacagg tacagggatt aaaacccaaa acatagatgc aacggttcag
       61 ggattgctga agttgaacga cagggaactt gctacacaag gaaatgtgat gcatgttacc
      121 aatactgcac cggctgcaat tacacgtaca gggggactaa actcaactcc tgttcagggt
      181 tttgtgagca gcacaggcag tggtaagttg tggcgcaaca ccaacgctac atctgtgtat
      241 ttatttcctg taggttcttc aattcctcca gctcgttttc gtcctgtaga aattaagcca
      301 ttaagtgcaa cacctaatac ttttgctgtg cgtatggtta ataacgaccc gaatactgat
      361 ggttttaacc gtgctgtgcg tgaaccaact ttgggtgctg taaatgcagg atggtatcag
      421 aaaataaaca gagtttcggg tagttcatta gcagagtttt ctctttatgc cgatgatgca
      481 caggatgcca ttgcttcctg gcctttactt aaactttgtc agtgggggca ggcagcccct
      541 cctatatggt ggaaagatat tggcggtgct gtaactccgg gtgcttcgcc tgtgttaggt
      601 tcggtgttgc gtaccaactg gaataatttc agcaacacag aaaatttcaa tattgcaccg
      661 gaatcaaatc cactgcctgt tgagttacta tcgtttacag gaagttgtaa gaacaacatt
      721 gttaatctga attggacaac tgcatctgaa ataaacaatc attattttga gttgcaacag
      781 tcggaagatg gaagcaactt tgtttctatt ggtcgtgttg aaggtaatgg aactaccatc
      841 aaacaaaagt tttattcttt taccattgac caaaaaactg agcgcatatt tttccgattg
      901 gtacaatatg attttgatgg ccgggcaaat gtgctgcctg caattttagt ttcatgcaac
      961 acagaaaatc caacaacagt aaacattaac ctgatgccaa atccggcaaa agatgttttt
     1021 gtttctgaca tttatcttcc tgaaaaaaca gaactctcaa tgaaggtatt taacaccatt
     1081 gggcaattag ttttggaaga acaatggaca ctggatgcag gataccataa gcaggcaaca
     1141 caagtttcta ctcttgcaaa agatatttac aacatacgct atacttcaaa cacatttaac
     1201 atagtgaagc aaatagctat agtaaaataa ttgttgatgg cagaaattgc attgcgtagt
     1261 ttgcaaattg aagatgcaga attatttgct ttgcatgcca ataacaatgc aattgctaat
     1321 aacatgacca atcagtttcc ctttccttat acaaaagcgc atgcattgga atttatcaga
     1381 cgggttaacg gtttaaaacc tgctcaggtt tttgcaatag atttagatgg tgaggcaata
     1441 ggtgctattg gactgcatcc tcagactgat atttttctaa aaaatgcaga acttggatat
     1501 tggcttgcag aaaaatattg gggcaaagga tttgtttcca ttgctattaa aaagattgtt
     1561 gaatatggct ttagcaattt cgaaatagat agaatttttg cacgcccttt tggaagcaat
     1621 aaagcatcac aacgagtcct ggaaaaaaca ggctttattc tggaagcccg ccttgaaaag
     1681 attattttta aaaataatcg ttatgaagat gagctaattt atgcaatccg aaaaagtcat
     1741 aatcatccaa aaacctgagg tcggatgcta aaaccttatc ttaaaacata ggaagaagca
     1801 ccactgaggt tgccatcaat atacacttca atcagatagg taccgggtgt aaatattcta
     1861 tcgttctcct cataactcag gcaattgtca actgtggcat tttcgtagcg gagggatgaa
     1921 acaactgtac acattaactc ttcgccacta gccatcttaa acgatgctga tccggcagag
     1981 cggttaccca taaccttacc acccggctca atgattctca gataaacatt tttttctcct
     2041 gccttggcaa ttttattttc aagaacagaa aaacatacat ccattttatt ggtgcgtttt
     2101 gataacgctg tggacacaaa tttaccattg ccttttttct tgaaagtaga aactttgaaa
     2161 tattcggact ttaaaaccga tgcagtgttc actgttgttt ctaaattttt agaaagtgtt
     2221 tccacagtac ctgataattg tgcattatca tccttgagct tttgattttc caccatcaag
     2281 gtatcaatct tatccaaata ttcatcacgc agttttttga gtgactccaa ttctgccatc
     2341 agctttttat ttaatgcagc actgttgcct tcgcttctaa ttaatttctg aatacgttct
     2401 ttctgttcat caattttatg attggcctca gttaatagac tgtctaaatt gtcattaata
     2461 cctttgtatt tgtttagctc ttcgatggtg gcacccaatt ctttttctac atcaacacgt
     2521 gctgtaatga ggctgtcttt agtaacctca aatacctctg tggtagatgt atgattgcgc
     2581 cattgatata cgttaaacaa tgccgaagca gcaaataaaa caatccaaat acgattattc
     2641 cctttactct ggttttccat gattattttt ttatagatta aaaaacaaag gtaatacgta
     2701 ttttaaagta aaaaagccat gtgtgtcaag gttttgaaca ggttcattgt caacaaaaag
     2761 gcataaaatc agaacccgaa acgggcactt ggagccacat caagtgttga aaaatcagca
     2821 gccagatatt tgaaatatgc tgcaagacaa atcatgccac cattatcggt acacagcgaa
     2881 agtggcggga tatgcacctg ccaattactt ttaacggcct cttctgtaat tgcttttcgt
     2941 agcccactgt ttgccgacac gccaccggca atggcaatat tgttgatacc tgtttctatg
     3001 gctgctttct ttaatttaag cagaagtgtc tttacaatgg tatattgcac ggaagcacat
     3061 aggtcatctc tgttttgtgt aataaaagca ggattctgca caagttgctt ctttaaaaaa
     3121 tacaagacag aagttttcag accactaaag gaataattga gccccggcag gtttgctaca
     3181 ggaaagtgaa atgcttttgg attgccgctc agtgcatatt tatcaatgag tggcccaccg
     3241 ggataaggca actctaatac tttggcaatt ttatcaaatg cttcaccggc agcatcatca
     3301 agcgtctgcc cgataatttc catttgatgc gatgaagtta ctttgacaat ttgtgtgtgt
     3361 ccacccgaaa cggttaaaca taaaaatgga aatgatggag ttctgccaat ctgcccttct
     3421 atgcttaaaa aattagctag cacgtgtgcc tgcatgtggt tgacttcaat gagtggtttg
     3481 tttaatgcag cacctaaaga cttggcaaat gaaacgccta ccatgagtga gccaatgagt
     3541 ccgggtccac gggtaaaggc aatggcatca acatcattaa gatgaatggc tgcttcttgc
     3601 aaggcctgct ccaccacggg aacaatgttt gactgatgtg ccctgctggc taattcagga
     3661 accacaccgc catatttttg atgtacggtt tggttggcaa tgacatttga caaaatatag
     3721 gtattccgac ttacggcagc agaggtttca tcgcaagatg attcaatacc aagtatgaca
     3781 atgtcttttt tatccattaa cgtcatcgcc tttttcacta tacatcagag cacaaagtta
     3841 agaggaaata tcagtttatc agacaggctt caaaacacat tacataaact atcatgatgc
     3901 aatgaattta aggtaaacta acatggatgt ggtattattt ttagggcagc aagagtaata
     3961 acacttaaat aattacgcta tttttatccg cttcaaaacg acagggtatc aggaagtttt
     4021 ttaaaatatt actaattaca ataggcgcat tactgctgct gcttttttta ttttctgtca
     4081 gcttgagatt tcaaaaagtt caaagttggg tagttgatcg tgttaccggt tatttgtcat
     4141 ccgaattaaa tacaacagta catattggcg gagtaaggtt tgtgttgatt aaaagtgttt
     4201 accttgatga tgtatttatt gccgacaagc aacaggatac gctgttgtat gccaaacacc
     4261 ttacagcgac agttgctttt caatcattat ttatccaaaa taaaaaacta atacttgatg
     4321 aagtgcagtt agacaaagca cgaatcgcct tatcaaagtc gaagagcaca cgagatatga
     4381 acttcgattt tattgtggat tatttcagtt caccttcaaa ggacacaaca acatcaccat
     4441 tttattttgc tgtaaacaga gtt
//
LOCUS       DAOQNP010000130         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_8647_length_4431_cov_7.798675, whole genome shotgun sequence.
ACCESSION   DAOQNP010000130 DAOQNP010000000
VERSION     DAOQNP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8647_length_4431_cov_7.798675"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /gene="sppA"
                     /locus_tag="PKY09_12095"
     CDS             complement(<1..1589)
                     /gene="sppA"
                     /locus_tag="PKY09_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNB13634.1"
                     /translation="MKQFLKYVLATLTGLILFCLLTFFLFAVMLGSAFKKEVVEVKNN
                     SVLKIEFKMPIAERSDNNPFRNLNFSTLKASKQPGLNDIISNIEKAEKDDNIKGILLN
                     LNSFEAGYATTTEIRNALVKFKKSGKFLFAYAEYYSTKAYYLASAADKIYVHPEGAVD
                     FKGLYAQLMFMKGALDKLEIEPEIIRHGKFKSAIEPFILDKMSPENKEQVATFINSIW
                     ETISSEIAESRKISREALEQISDSLSAENAKDALRLKLVDQLCYKDELEKILAEKSDV
                     KEDEINYISLNKYNEAVVKSDKKFTTDRIAVIYAVGDIVSGSGEDDQVGSDRIAAAIK
                     KARLDDKVKAIVLRVNSPGGSALASDVIWRETKLAKEKKPFIVSMGNLAASGGYYISC
                     LADTIVAQRTTITGSIGVFGLLFNAQKLLNNKLGLTFDTYKTGPYADLGMPTRPLEPA
                     ERNILQRSVEDVYSTFIGRVAEGRKMETAMVDSIGQGRVWSATDAKKIGLVDLYGGLN
                     DAVVLAVKKAGLDNYRIKELP"
     gene            1647..2156
                     /gene="folK"
                     /locus_tag="PKY09_12100"
     CDS             1647..2156
                     /gene="folK"
                     /locus_tag="PKY09_12100"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNB13635.1"
                     /translation="MASHQNIAIILAGSNLGNRLHNLSAAAMFLEKLSTRKIDCSSVY
                     ETAPWGNTQQPPFLNQAFRVYTPLTANELLDELLAFEKSQGRTRLQQWEPRSIDLDIL
                     FYNHDLISTPELTIPHKHLHARRFVLEPLAELIPDFIHPVFGKNITELLELCNDELKV
                     TKFPVYAQK"
     gene            2143..2760
                     /locus_tag="PKY09_12105"
     CDS             2143..2760
                     /locus_tag="PKY09_12105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNB13636.1"
                     /translation="MHRNKFLTIEGNIGAGKTSLARMIADEFNFQLILEEFAENTFLQ
                     QFYESPEKFAFPLEMSFMAARYKQLKTFFNKNTLPFPIVSDYMFQKTLLFAKSNLKNN
                     EYGLFEQFFQMLNVGLPLPQLIVYLQKEIPSLQKNIKKRGRDYEQNMKDDYLLKINNA
                     YGRFMKKPNAASNILIINTEGLDFVRKRSDFQKILETIEKAGFRF"
     gene            2876..4330
                     /locus_tag="PKY09_12110"
     CDS             2876..4330
                     /locus_tag="PKY09_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNB13637.1"
                     /translation="MKVKTLLVSAFLITATQFAFAQESARYGLPKNKDFDKWSAGITV
                     GLNWFQGDLNEKTGNNNSILKNIQDPVFGLKVGYQISHSVQVNVRGNYTNLSGKKNKT
                     TFRVPNAVTSPQITRNIKFKSPVYQGSLNLNYTIGNISFLQRNKRLHFYGEIGLGIFS
                     YAPKVTDLDNGTVYVKKGSVAEGFLPLSLGFKYQIKRFDIGLLATFNKTLNDKVDQVY
                     DSKTESDNWSFFQLGLNYTFGKKQAMMEWVNPMEVVYNDLSDMKDRIDVMSGDKDKDG
                     VSDLFDKDNSTPEGVKVYGDGTALDSDGDGVPDNVDSDPYSNKGAKVDASGLEVDTDG
                     DGVPDSRDMEPNTPKGNLVNFQGMTIAPAGSYGNDGITSNSATGWLPSIFFDVDQSAI
                     KSVYHDRLNTVARVMIKNPKLKLNVVGNCDVDASEQYNEKLGMKRAEAAVNHLVKVYG
                     IDASRLTATSLGKKEQLAKGNKRMNRRVDFEVAK"
BASE COUNT         1370 a          844 c          811 g         1406 t
ORIGIN      
        1 tcaggaagtt ctttaattct gtaattgtct aaccctgctt tttttacggc aagcacaaca
       61 gcatcattca ggccgccata caggtcaacc aaaccaattt tttttgcatc tgtagcactc
      121 cacactcttc cttgtccaat gctgtcaacc atagccgttt ccatttttct tccttcggca
      181 acacgtccta taaaagtgct gtaaacgtct tcaacactgc gttgaagaat atttctttca
      241 gcaggttcaa gtggtcttgt tggcatacca aggtcggcat aaggtccggt tttatatgtg
      301 tcaaatgtca atcctaattt gttgttgagt agtttttgtg cattaaagag caaaccaaaa
      361 acaccaatag agcctgtaat ggtggtgcgc tgcgcaacaa tcgtatcggc caaacaactg
      421 atgtagtagc ctcctgaggc agcaagattg cccatactta ctataaatgg ttttttctcc
      481 ttagctaatt ttgtttcacg ccatattaca tcagatgcca acgcactacc tcccggtgaa
      541 ttaactctta aaacaatggc ctttacttta tcatctaatc gtgctttttt tattgctgct
      601 gctattctat cagaacctac ctggtcgtct tcgccactac cactaacaat gtcaccaaca
      661 gcatagatta cggcaatgcg atcggtggtg aattttttat ccgacttaac aacagcttca
      721 ttgtatttgt tcaggctgat gtaattaatc tcatcttctt taacatctga tttttctgcc
      781 agaatttttt ctaactcatc tttataacat aattggtcaa ctaattttaa cctaagtgca
      841 tccttagcat tttctgcaga cagactgtct gaaatttgct ctaatgcttc acgacttatt
      901 ttacgtgatt ctgcaatttc gctactaata gtttcccaaa tagaattaat aaaagtggct
      961 acctgttctt tattctcagg actcatttta tccaaaataa atggctcgat agcactttta
     1021 aattttccat gcctgataat ttcaggctct atttccaatt tatctaaggc tcctttcata
     1081 aacattaact gcgcatagag tcctttaaaa tctactgcac cttcgggatg aacataaatt
     1141 ttatctgctg cgcttgccag ataataagct ttggttgaat aatattctgc ataagcaaaa
     1201 agaaacttac cagatttttt aaattttact aaagcatttc tgatttctgt tgtagtggca
     1261 tagccggctt caaaactatt gaggttaagt aaaatgcctt taatattatc atctttttca
     1321 gctttctcta tattggaaat aatatcattg agcccgggtt gtttacttgc ttttaatgtg
     1381 ctgaaattaa gattgcgaaa tgggttgtta tcacttcgtt ctgcaattgg cattttgaat
     1441 tctattttta gtacggagtt gtttttaacc tctacaactt ctttcttaaa tgcactgcca
     1501 agcattacgg caaacaaaaa gaatgttaat agacaaaaaa gaatcaggcc ggtaagtgtg
     1561 gccaaaacat atttcagaaa ttgtttcatt ttaaatggac ttgtgtgagt ttgaaaattt
     1621 agctttgcaa agctataaaa atcaaaatgg cttcacatca gaatattgca attatacttg
     1681 caggcagcaa tttaggtaac agactgcaca acctgtctgc agctgcaatg tttcttgaaa
     1741 aactatcaac acggaaaatt gattgttcat cggtatatga aactgccccc tggggcaata
     1801 cgcaacaacc tccttttctg aatcaggcat tcagagtcta tactccttta actgcaaatg
     1861 aattacttga tgaattgtta gcatttgaaa aaagtcaggg gcgtacacgt ttgcaacaat
     1921 gggaaccacg cagtattgat cttgatattc ttttttacaa tcatgacctc atcagtacac
     1981 ctgaactgac aattccgcac aaacatttgc atgcaagacg ttttgtcttg gagcctcttg
     2041 ctgaacttat acctgatttt attcatcctg ttttcggaaa aaacattaca gaattgcttg
     2101 agttatgcaa cgatgaattg aaggtgacta aatttcctgt gtatgcacag aaataaattt
     2161 ttaaccatag aaggaaatat tggtgcaggc aaaacatcat tggccaggat gattgccgat
     2221 gagtttaatt tccaactgat tttggaagag tttgcagaaa atactttttt acaacagttt
     2281 tatgaaagcc ctgaaaaatt tgcctttcca ctggaaatgt catttatggc tgcacgatat
     2341 aagcaactga aaacattttt caacaaaaac acactgccgt ttcctattgt gtctgactac
     2401 atgtttcaga aaactttgct gtttgccaag tcgaatctta agaataatga gtacggcctt
     2461 tttgaacagt tttttcagat gcttaacgta ggattgcctt tgccacaatt aattgtgtat
     2521 ttacaaaaag aaataccctc acttcagaaa aatatcaaga aacgcggacg cgattacgag
     2581 cagaatatga aggatgatta cctgctaaaa atcaataatg cctatggtcg ttttatgaaa
     2641 aagcccaatg cggcttccaa tatcctaatt atcaatacag aaggtcttga ttttgtgcgg
     2701 aaaagaagcg attttcaaaa aatacttgaa actattgaaa aggcaggttt taggttctga
     2761 tttacaagtt atgtcaataa aactgtgcat atccttcata tttaatttta atatttaaaa
     2821 tcattgtgtt atacttgcac cacaaatttt gtaaaatatt aaaccactct atacgatgaa
     2881 agtaaaaacc ttactcgttt cagctttttt aatcactgcc acgcaatttg cgtttgctca
     2941 ggaatcagcc agatatggtc ttcctaagaa caaggatttt gacaaatggt cagcaggcat
     3001 tactgtagga ttaaactggt ttcagggcga ccttaacgaa aaaaccggaa acaacaacag
     3061 tatcctcaag aacattcagg accctgtatt cggtcttaaa gtgggctatc agataagcca
     3121 ctctgtgcag gtaaacgttc gtggtaatta taccaacttg agcggaaaaa agaacaaaac
     3181 aaccttcaga gttcctaatg ctgttaccag tcctcaaatt accagaaaca tcaagtttaa
     3241 gtcgcctgtg tatcaaggca gtcttaatct gaattatacc attggtaaca tcagcttctt
     3301 gcaacgtaat aagcgattac acttctatgg tgaaattgga ttaggtatat ttagctatgc
     3361 accaaaagtt accgatctgg acaatggaac tgtatatgta aaaaaaggct ctgttgctga
     3421 aggattctta cctttaagtt taggattcaa atatcaaatc aagcgttttg atattggctt
     3481 acttgccact ttcaacaaga ctcttaacga taaagtagat caggtatatg actctaaaac
     3541 tgagagtgac aactggtcgt ttttccaatt aggactaaac tatacatttg gcaaaaaaca
     3601 agctatgatg gagtgggtta acccaatgga agtagtttac aacgatcttt ctgatatgaa
     3661 agacagaatt gatgtaatgt caggcgataa agacaaagac ggtgtttctg atttgttcga
     3721 taaagacaac tctactcctg aaggcgttaa agtttatggt gatggtactg ccttggatag
     3781 tgatggtgat ggtgttccgg ataatgtgga ctctgatcct tattctaata aaggagctaa
     3841 agttgatgcc agtggtttag aagtggatac tgatggtgat ggcgttcctg acagccgcga
     3901 tatggaaccg aacacaccta aaggaaactt agtaaacttc cagggtatga ctattgcacc
     3961 tgccggttct tatggaaatg atggtatcac ttctaacagt gctaccggtt ggttgccttc
     4021 aatcttcttt gatgttgacc aatctgcaat caaatcagtt taccacgacc gtttgaatac
     4081 cgttgcacga gtgatgatta aaaatcctaa gttgaaactg aatgttgttg gtaattgcga
     4141 tgttgatgca tctgaacaat acaatgaaaa attaggtatg aagcgtgcag aagcagcagt
     4201 aaaccacctt gtaaaagttt atggtattga cgcttcaaga cttactgcca catcattggg
     4261 caagaaagaa caacttgcta aaggcaacaa acgtatgaac agaagggttg attttgaagt
     4321 agcaaaataa cctgaatgat atttaaggga aggccggact ttagtccggc tttttttatg
     4381 ctttcatttt ctgaattgca cctgtcagga ggcgaagggc cgacctgaca g
//
LOCUS       DAOQNP010000131         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_8821_length_4377_cov_8.465988, whole genome shotgun sequence.
ACCESSION   DAOQNP010000131 DAOQNP010000000
VERSION     DAOQNP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8821_length_4377_cov_8.465988"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKY09_12115"
     CDS             complement(<1..232)
                     /locus_tag="PKY09_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HNB13638.1"
                     /translation="MILGVPKELKNRERRVALTPDVVKSLVASGYNVVVEEGAGEASF
                     YTNDQYKNAGATLGGKAQVYGSDVVLKVNAPQP"
     gene            404..706
                     /locus_tag="PKY09_12120"
     CDS             404..706
                     /locus_tag="PKY09_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13639.1"
                     /translation="MAKFTPQYLKKLEEHLQENGYDVRYEKGNFKNGYCLLESKKLVM
                     VNKFSAMETRCSALLEIINLLESQQLLSNKRQELFAAMENPEPQVVTIPHSSESEH"
     gene            703..1467
                     /locus_tag="PKY09_12125"
     CDS             703..1467
                     /locus_tag="PKY09_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNB13640.1"
                     /translation="MKITFLGTGTSQGVPVIACNCNVCQSSDLRDKRLRTSIHIQYKN
                     SSFVIDSGPDFRQQMLRAQIKNLTALIFTHEHKDHVAGMDDIRAFNYVLQKKIDIYAT
                     LRVQEALVREFPYVFHDFKYPGVPEVNMITIDEHPFNIEGLEFIPIEVLHYKLPVNAY
                     RVGDFTYITDANFISEKEKEKIKGSRIVVINALRREKHVSHFNLEEALELIKELKPEK
                     AYLTHISHQLGKHSDVEKELPPNVFLAYDGFEIEMS"
     gene            1528..2412
                     /locus_tag="PKY09_12130"
     CDS             1528..2412
                     /locus_tag="PKY09_12130"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNB13641.1"
                     /translation="MQVTIDSNSGFCFGVVYAIQQAEEELDVNGHLYCLGDIVHNNME
                     VDRLKGKGLEIINHEQLQQLHNCKVLIRAHGEPPSTYQTAIKNNIELIDASCPVVLKL
                     QNRVRGSYDEITDTGNGQVVIYGEKGHAEVNGLVGQTGGKAIVVKTEEDLDQIDFNKP
                     VHFFSQTTKSTLGFEQMQKTIQQRIVKQKGSLEETDFITNDTLCRQVSNREPQMRRFS
                     SQHDVVVFVSGKKSSNGKALYSVCKATNDNSYFISSAEELDATWFKGINSVGICGATS
                     TPLWLMKEVKNAIEKITV"
     gene            complement(2506..>4377)
                     /locus_tag="PKY09_12135"
     CDS             complement(2506..>4377)
                     /locus_tag="PKY09_12135"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13642.1"
                     /translation="ATNGTSPICISSPSSVITLTGSAATLPIGDYIVTYDRSLPSGSN
                     LTASMSVLTAGTGSFTATGLTSAGNSTITVTNLTSSYCSSNISTNNTTVINVVGGTPA
                     QPDVIAGAPANVCPPTGSYTLSTSSTGADSYNWYLGTGVNGVTFLSANGTNTMDVQFG
                     TTSNSTYVIRVDATNVCGTSAYRPVLIRRATSVPSAISGLKTACASTSDTYTATAVDG
                     ATEYVWSISGDASVTGNTNSVTVNFGPAWTGGTLCVASKVGCFTSATKCMAITTSATP
                     LSTVLAGTFKACPNASLNFNVTATPGVATYNWTVPANASINSGQGTNTITVDFNSNYN
                     NTGNICMTATSICGVVAAPKCKSVSPGLPAVPATITGGTNGLCNQTMPFSTPMVAGVT
                     SYNWTAPGTVNGNGNNAVTIDFGALTTGQVCVSASNACGTSSTRCTAIKGAPNTPASI
                     TATPNTWCPNTAGVEFNANIANVTGAYTLSWTYPETSANYVSGGGNSSSLTLDWTTGN
                     GAVMLMASNSCGSGTKVFNVVLDNCREAGIETAKQINVYPNPANDMVNISYQSTTTEL
                     IKVSMMDYSGKLVINQNVQVTSGMNNYTLDVSKMASGVYMLNIQSESGNMQRKLIKQ"
BASE COUNT         1347 a          864 c          879 g         1287 t
ORIGIN      
        1 ccggttgcgg tgcattaacc ttcaacacta catcacttcc atatacttgt gccttgccgc
       61 caagtgttgc acctgcattt ttatattggt cgttggtata aaaagaagcc tcaccggcac
      121 cctcttcaac aacaacattg tagccggaag ctactaaact tttaacaaca tcaggggtta
      181 gtgctactcg cctttcgcga ttttttaact ccttgggtac gcctaaaatc atgcccttaa
      241 ttttttgatt aacaatttat cagaaagcgg tgcaaagaaa ataattttcg gcttatatgc
      301 aatctcttat caatgttgtc cgttgttaat aaaactccct gccagagcaa aatagtgaac
      361 atcatactca ataattttga cacagagaac ctctaaaaac aatatggcta agtttacacc
      421 acagtattta aaaaaattag aagagcattt acaggaaaac ggctacgatg tacgctacga
      481 aaaaggcaac tttaaaaatg gttattgttt gctggaatcc aaaaagttag tcatggtaaa
      541 taaattttct gccatggaaa cacgatgcag tgccttgctt gaaattatta atcttctaga
      601 atcgcaacaa ttactcagca acaaaaggca ggagttgttt gcagcaatgg aaaatcccga
      661 accacaggta gttaccatac ctcattcatc agaatctgaa cattgaaaat aacttttttg
      721 ggtacaggca cttcgcaagg cgttccggta atagcctgca attgcaatgt atgtcaaagt
      781 tcagatttgc gcgacaagcg attgcgcact tccattcata ttcagtataa gaacagcagt
      841 tttgtcattg actccggccc cgatttcagg caacagatgc tgcgggcgca gataaagaac
      901 cttactgcat taatatttac ccacgagcat aaagatcacg tggcaggtat ggacgacatc
      961 agggcattca actatgtgtt gcagaaaaaa attgacatct atgctacttt gcgtgttcag
     1021 gaagcgttgg taagagagtt tccctatgtt tttcatgact ttaaatatcc gggtgtgcct
     1081 gaagtgaata tgattaccat tgatgagcac ccttttaata ttgaaggact tgagtttatt
     1141 cccattgaag tgctgcatta caaattacct gtcaatgcct atagggttgg tgattttact
     1201 tacataacag atgccaactt catttcagaa aaagaaaaag aaaaaattaa aggcagtcgc
     1261 attgtagtta tcaatgcttt gagacgcgaa aaacatgttt ctcattttaa tctagaagaa
     1321 gcacttgagt tgattaaaga attgaagccc gaaaaagcct acctcacaca cattagccat
     1381 caacttggaa aacacagcga tgtagagaaa gaattgccac caaatgtgtt tttagcttac
     1441 gatggttttg aaatagaaat gtcataagat taaaccttat ggcctttaaa acgttattgt
     1501 cttacttttg cacataattt ttccactatg caggttacta ttgattccaa ttcaggcttt
     1561 tgctttggtg ttgtatatgc tattcagcaa gccgaagaag agttggatgt gaacgggcat
     1621 ttatactgtc ttggcgacat tgtacataat aacatggagg ttgacagact taaaggcaaa
     1681 ggcctggaaa taatcaatca tgaacaattg cagcagcttc acaactgtaa agtgcttatt
     1741 cgtgcacatg gcgaaccacc ttcaacatat caaacggcaa ttaaaaacaa tattgagctt
     1801 attgacgcat cttgtcctgt tgtattgaag ttacaaaatc gtgtcagagg cagctatgat
     1861 gaaatcacag atacaggaaa tggccaagtg gtgatttatg gtgagaaagg tcatgctgaa
     1921 gtgaatggct tggtggggca gacaggaggt aaagccattg tggttaaaac tgaagaagat
     1981 cttgatcaga ttgatttcaa taaaccggtt cattttttct ctcagacaac aaaaagtaca
     2041 ctcggctttg agcagatgca aaaaaccatt cagcaacgaa tagttaaaca aaaaggctcg
     2101 cttgaagaaa ccgattttat taccaacgac actttatgcc gtcaggtcag caaccgcgaa
     2161 ccacagatgc gcagattctc atcacaacat gatgttgtag tttttgtaag cggaaaaaaa
     2221 tcatcaaacg gaaaagcgtt gtacagtgtc tgtaaagcta ccaacgacaa tagctatttt
     2281 atttcatcgg ctgaagagtt ggatgccaca tggtttaagg gaattaattc tgtaggtatt
     2341 tgtggagcca cctcaacacc actatggctg atgaaggaag ttaaaaatgc cattgaaaaa
     2401 attacagtct aatcattttt attttcttct gattaaaaat caggcataaa acaaacgagg
     2461 ctgcttcgta accgaagcag cctcgtttgt ttcaaaccaa ttcaattatt gctttatcag
     2521 cttgcgttgc atattgccgc tttctgattg tatgtttaac atataaacac cagaagccat
     2581 ttttgacaca tctaaagtat agttgttcat tcctgaagtt acttgcacat tttgattgat
     2641 tacaagttta ccggagtaat ccatcatcga aacttttatc aactctgttg ttgttgattg
     2701 atatgaaatg tttaccatat catttgcagg attaggatac acattaattt gtttggctgt
     2761 ttcaattcct gcttcgcggc agttgtctaa aacaacatta aacactttag ttcccgatcc
     2821 acatgaattt gatgccatca gcattacagc accgtttcct gttgtccagt caagtgtaag
     2881 tgaacttgag ttaccaccac cggaaacata attggcagat gtttcagggt atgtccagct
     2941 taaggtatat gcaccggtta cgttggcaat gttagcatta aactcaactc ctgctgtgtt
     3001 tggacaccat gtatttggtg ttgcagttat tgaagccgga gtatttggcg cacctttaat
     3061 tgcagtacaa cgtgtactgc ttgttccgca tgcattggat gcactcacac aaacctgtcc
     3121 tgttgttaaa gcaccgaaat caattgttac tgcattattt ccattaccat taacagttcc
     3181 gggtgctgtc cagttgtagc tggttacacc tgccaccatt ggtgttgaaa atggcatagt
     3241 ttgattacac aatccattgg taccaccggt tatggttgca ggcacagcag gcaatccggg
     3301 agaaacactc ttacacttag gtgctgcaac tacgccacaa atgctggtag ctgtcataca
     3361 gatattacct gtgttgttat aattagaatt aaagtcaacg gtaatagtat ttgtaccttg
     3421 tccactgttt atagaagcat tggcaggcac tgtccagtta taagtagcca caccgggtgt
     3481 tgctgtaaca ttaaaattaa gcgatgcatt agggcaagct ttaaaggtac ctgccagcac
     3541 tgtacttaaa ggagttgctg atgttgttat tgccatgcat tttgttgcac tggtaaaaca
     3601 tcccactttt gaagcaacac aaagtgtacc acctgtccat gcaggaccaa agtttacggt
     3661 aacactgttt gtatttcctg ttactgaagc atcgccactt attgaccaca catattctgt
     3721 agcaccatca acagcagttg ctgtataagt atctgaggtg ctggcacagg cagttttaag
     3781 tccgctgata gcagaaggaa cagaagtagc tcttctgatt aataccggac gataggctga
     3841 tgtaccacaa acatttgttg catcaacacg aatgacatag gttgagtttg aagtggtacc
     3901 aaactgaacg tccatagtgt tagttccatt ggcacttaaa aatgtgactc cgttaacacc
     3961 cgtacccaaa taccagttgt aagaatcggc acctgtactg cttgtactta aggtatagct
     4021 tcctgttgga ggacatacat tggcaggggc gcctgcaata acatccggtt gtgccggagt
     4081 accacctaca acattaatca cagtagtatt attagtagaa atattgctgc tgcaataaga
     4141 tgaagttaaa ttggtaactg taattgtgct gttacctgct gaagttaaac ctgtggcagt
     4201 aaatgatcct gttcctgctg taagcactga catagaagcg gtaagattag atcctgaagg
     4261 aagacttcta tcgtaagtaa ctatataatc accaatgggc aatgttgcag cactaccagt
     4321 taaagtaatg acactacttg gcgaagaaat gcaaattgga cttgtgccat tagttgc
//
LOCUS       DAOQNP010000132         4314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_9019_length_4314_cov_4.784222, whole genome shotgun sequence.
ACCESSION   DAOQNP010000132 DAOQNP010000000
VERSION     DAOQNP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9019_length_4314_cov_4.784222"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PKY09_12140"
     CDS             complement(<1..1202)
                     /locus_tag="PKY09_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13643.1"
                     /translation="MKKYILCYCLIVFTTGLFAQKQNAVWVFGNNAGIDFNSNPPTFR
                     SGSVNNSLDNTSAISDSAGNLLFYTDGQNIYTANGAIMNSGAPLMGSSNGGQCALIVP
                     RPNSTLYYVFTVDQYNNNNGCRYSIVDMSLNNGSGAVVSANNLLFTPSTEKLEAVYNC
                     NGNFYWIITHLWGTDQFFTFKLDGSGLIAMPVISQVGSVHANAFNNAMGQMTLSPDKQ
                     RLACAIYDDGLIELFDFDINTGVLSNAITLTGFTHPLGVAFSPNSEFLYYTQLFTSNI
                     YQIDVTLASATIIQNSTVVIGTVSGPGTQGYLSCYMQLAPDGKIYVASFDDNFLGVIS
                     NPNAQGMLAAVIDNSFYLPGAICYGGVCRSVSDYCAETAVSEIINQNAVSLFSTEGEG
                     SLVIKNNS"
     gene            1271..2740
                     /locus_tag="PKY09_12145"
     CDS             1271..2740
                     /locus_tag="PKY09_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNB13644.1"
                     /translation="MGVIQRQGIKNTITTYLGIAIGFISLIVIQPQFLTKEELGLTRI
                     LYSFSMLMAMFVPLGIGNATTKYFPLFKDGNKNHHGYLAFMLLFPALGFVVTAVVLWL
                     LKDFIVGQYVKESPLFTEYFYYVFPLTFILALISCLNVYCYANYKSTIPAFLNDVVAR
                     LLVIAVITTYFLKWITLQQFIACYVFLYGLQLLLLLAYIFQFDKPKFKIDIPYFREKK
                     FLQLVNYGLLLWFAGVASIGLKYLDSIMLGKYLPLSFVGIYTIAAFIPTVIEAPLTAL
                     EKIAASRIAFAWAEDNRKDILEIYRKSSLYMLALGGLLFTGININIVNLLSFLPEGYQ
                     QGASVVLIISFGTLINMITGANAPILFNSDKYRWGAFFLIALALVSVVLQMVFIPLLG
                     LNGAAIATVACYVLYNVFMSAVVWKFYHLHPFDKKMLVIVAAMLLCFGISYLLPSIEN
                     KIIDMIYRSVIVTVIYLMMIYRFKVLEEFHHLLPWQRKI"
     gene            complement(2741..3841)
                     /gene="ychF"
                     /locus_tag="PKY09_12150"
     CDS             complement(2741..3841)
                     /gene="ychF"
                     /locus_tag="PKY09_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNB13645.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNIGVI
                     TVPDDRLNKLETLVKPQRVVPTTVEIVDIAGLVKGASKGEGLGNQFLGNIRETDAIIH
                     VLRCFDDPNVVHVDGSVNPVRDKETIDFELQIKDLEAIDKKIQRIEKIARVGNDNDAK
                     KAYDVLQVFKKHLESGNAARSAPVQPEDFQYVADIFLLTAKPVLYVCNVDEKSVLTGN
                     KHVDAVREKVKDENAEVLVIAAAIEAEITQLETFEERQMFLTDLGLTESGVAKLIKAA
                     YRLLQLQTYFTAGEKEVRAWTITKGMTAPQAAGVIHSDFEKGFIRAEVIRYNDFITLG
                     SEQACKENGKLSVEGKEYVVNDGDVMHFRFNV"
     gene            complement(3912..>4314)
                     /locus_tag="PKY09_12155"
     CDS             complement(3912..>4314)
                     /locus_tag="PKY09_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13646.1"
                     /translation="AGVVNGMFASVNKATGVSLSIPDPIKVKVNMGGTVQKPEIKTSL
                     ADGGKGLVEGVKEKAMEQLDAKKQELEDKAKAEAERLKKEAEEKVKSEADRLKKEAEA
                     KAKAEADRLKKEAEERAKKEAEDKLKNIFGK"
BASE COUNT         1194 a          925 c          770 g         1425 t
ORIGIN      
        1 tgactattat ttttgataac taaacttcct tccccttctg ttgaaaataa acttacagca
       61 ttttgattta ttatctcgct gacagcagtt tcagcacagt agtcagaaac acttctgcaa
      121 acacctccat aacatatagc cccaggcaaa taaaaactat tgtctattac agctgccaac
      181 atgccctgtg catttggatt gctgattact cccaaaaaat tatcatcaaa cgatgctaca
      241 taaattttac catcgggcgc caattgcata taacatgata aatacccttg tgtaccgggt
      301 ccggaaactg ttccaatcac aacggtactg ttctgaataa ttgttgcact tgcaagtgtt
      361 acatcaattt gataaatatt tgatgtaaat aactgagtat aatataaaaa ttcactgttg
      421 ggagaaaatg ctactcctaa tggatgtgta aaccccgtta aggtgatggc attggaaagt
      481 acaccggtat tgatgtcgaa gtcaaaaagt tcaatcagtc cgtcatcata gatggcacag
      541 gccagtcgtt gtttatctgg cgacagggtc atttgcccca tagcattatt aaacgcattg
      601 gcatgtacac ttccaacctg actgatgaca ggcattgcaa ttaaaccact tccatctaac
      661 ttaaaagtaa aaaactggtc agtgccccac aggtgtgtaa taatccagta gaagttgcca
      721 ttgcaattat agacagcttc taacttttct gttgagggtg taaacaataa gttattggca
      781 gacaccacag caccgctgcc attgttcaaa ctcatatcca caatactgta gcggcaaccg
      841 ttattgttgt tatactgatc aaccgtaaaa acataataca acgtgctgtt gggacgcggc
      901 acaattaagg cacactgccc gccattgctg ctacccatca aaggagcacc gctattcatt
      961 atagctccat tggccgtata aatgttttga ccatcggtat agaacaataa attaccagcc
     1021 gaatcggaaa tggctgaggt gttgtccaaa ctattgttga cagaaccact tctgaaagtt
     1081 ggtggattgg aattaaaatc aatgcctgca ttattaccaa atacccaaac ggcattttgt
     1141 ttttgtgcaa acaaccctgt tgtaaaaact atgaggcaat aacagaggat atactttttc
     1201 atagcagcat cattaaattt attactcttg taggcaaata taaaagaaaa aaaacagaaa
     1261 aagaacagta atgggtgtaa ttcaacggca gggtattaaa aacaccataa ctacctatct
     1321 gggtattgcc attggcttta tcagcctcat tgttattcag ccgcagtttc ttacaaaaga
     1381 agaactgggg cttacacgca ttctctattc cttttctatg ctcatggcca tgttcgtgcc
     1441 attaggcata ggcaatgcca ctaccaaata cttcccgctt tttaaagacg gtaataaaaa
     1501 tcatcatggc taccttgctt ttatgctttt gtttccggca ttgggctttg tggtgacggc
     1561 agttgtattg tggctgctca aagattttat agttgggcaa tatgttaaag aatcgccact
     1621 ctttaccgaa tatttttatt atgtatttcc actaacattc attctcgcac tcatatcgtg
     1681 ccttaatgtt tactgttatg ccaattacaa gtccaccata cctgcttttt taaatgatgt
     1741 ggttgcacgt ttgctggtga ttgctgtcat taccacctac tttttgaaat ggattacact
     1801 gcaacagttt attgcctgct atgtgttttt atatggtttg cagttgttgt tgctattagc
     1861 ttatattttt cagttcgaca agccaaagtt taaaattgat attccttatt tccgcgaaaa
     1921 aaaatttctt cagttggtca actacggact gctgttgtgg tttgcaggag tagcttcaat
     1981 aggattaaaa tatttagaca gcatcatgct tggcaaatat ctgcccctga gttttgtagg
     2041 tatttacacc atagcagctt ttattccaac agtgattgaa gcaccactta cggcattgga
     2101 aaaaattgcg gcctcgcgca ttgcatttgc ctgggctgaa gataacagaa aagatattct
     2161 tgaaatatac cgcaaatcat cattgtatat gctggcatta ggcggactgc tttttacagg
     2221 catcaacata aacattgtga atttgctgag ttttttacct gaaggctatc agcagggagc
     2281 ttctgtagta cttattatca gcttcggaac tttaatcaat atgattaccg gtgccaatgc
     2341 acccattctt ttcaattcag ataaatatcg ttggggtgca ttttttctta ttgccttagc
     2401 ccttgtgtct gttgtgctgc aaatggtttt tatccccttg ctgggactta acggtgctgc
     2461 cattgccact gttgcttgtt atgttttgta caatgtgttt atgtcggcag tggtttggaa
     2521 gttttatcac ctgcatccat ttgataagaa aatgctggtg attgttgctg ccatgctatt
     2581 gtgttttggc atcagttacc tattgccttc aatagaaaat aaaattattg atatgattta
     2641 tcgttctgta atagttacgg taatttattt aatgatgata taccgcttta aggtgcttga
     2701 agaatttcat catctgttgc cgtggcaaag aaaaatctaa tcagacgttg aagcggaagt
     2761 gcattacatc gccatcgtta accacatact ctttaccttc aacagaaagt tttccgtttt
     2821 ctttacaagc ctgctcagag ccaagcgtaa tgaaatcgtt atagcggata acttcggcac
     2881 gaataaaccc tttttcaaaa tcggagtgta tgacccctgc tgcctgaggt gctgtcattc
     2941 ctttggtgat agtccatgca cgcacttctt tttcaccggc agtaaaatag gtctgcagtt
     3001 gcagcaaacg atatgctgcc ttaatgagtt ttgctacacc tgattccgta agtcccaaat
     3061 ctgtcaaaaa catctgacgc tcttcaaagg tttccaattg ggttatctct gcttcaatag
     3121 cggctgcaat aacaagcact tctgcattct catctttcac tttttcgcga acggcatcaa
     3181 catgtttgtt gccggtaagt acagattttt cgtccacatt gcaaacgtac aatacaggtt
     3241 ttgctgtaag taaaaatatg tcggctacat attgaaaatc ttcaggctgc acaggtgcac
     3301 tgcgtgctgc attgccgctt tccaaatgct ttttaaaaac ctgcaatacg tcataggctt
     3361 tttttgcatc attatcatta cccacacggg caattttttc tatacgctga atttttttgt
     3421 caatagcttc aaggtctttt atctgcaatt caaagtcaat agtttcttta tcacgcaccg
     3481 gattcacaga accgtcaaca tgcactacgt tgggatcgtc aaaacatcta agtacatgga
     3541 taatagcatc tgtttcacga atgtttccca gaaactgatt ccccaaacct tctcccttgc
     3601 tggcaccttt caccaatcct gcaatgtcaa caatttcaac tgttgtgggt acaacacgtt
     3661 gtggctttac caatgtttct aacttattca atctgtcgtc aggaactgta atgacaccta
     3721 tattgggttc aatggtgcag aatggaaaat ttgcagcctg cgccttggca ttacttaaac
     3781 aattaaacag cgttgatttt cctacattag gtagtccaac aataccacat ttcaatccca
     3841 tagtaaaatt tttgagggtg caaagataac acttttaccc tctgaaaaaa tgatttaagc
     3901 gctttaggaa attacttacc gaaaatattt ttcaacttat cttcggcttc ttttttagct
     3961 ctttcctctg cttctttctt taaacgatct gcttctgcct ttgctttagc ttcggcttct
     4021 ttctttagtc tgtctgcttc ggattttact ttctcctctg cctctttctt taaacgctct
     4081 gcctcggctt ttgccttgtc ttccaactcc tgcttttttg catctaactg ctccatagct
     4141 ttttccttaa caccttcaac caaacctttt ccgccatcgg caagtgatgt ttttatttct
     4201 ggcttttgaa cagtgccacc catatttact ttaactttta taggatcggg aatgcttaat
     4261 gaaacacccg ttgccttatt gacacttgca aacattccgt taacaacacc tgct
//
LOCUS       DAOQNP010000133         4252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_9263_length_4252_cov_7.526805, whole genome shotgun sequence.
ACCESSION   DAOQNP010000133 DAOQNP010000000
VERSION     DAOQNP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9263_length_4252_cov_7.526805"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKY09_12160"
     CDS             <1..350
                     /locus_tag="PKY09_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13647.1"
                     /translation="TTKEELQDQAPSSALASLFFGFTNYLLASIILINPFKYIPYNLP
                     VFGTIFIGVNALFYFANRKLLVKAKKYIEIEHYYDKNNKLKKIHFMLITTFYMLGSIV
                     TMILAGIKYATSN"
     gene            complement(854..2899)
                     /locus_tag="PKY09_12165"
     CDS             complement(854..2899)
                     /locus_tag="PKY09_12165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017843030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNB13648.1"
                     /translation="MRKSILLAGFLTLASAGFAQNKLTPELLWKLGRVGDAVLSPDGK
                     TVVYTVRNFNLSGNNGNTDMYTVAVTGGEPKLIAGSADNESSPAWSADSKKIYFLNDK
                     GGANNLWCINADGTGEKQITTTADDISCFGISKAGNRIWMAKEVKVDKTTQDLYPDLP
                     KATGKIYDDLMYRHWTEWEDGSYSHVFIADFENDAVGELKDIQKGERFDTPMKPNDGG
                     EQIAWSADGNKIAYTCKKLAGKDYAVSTNSDIYLYDVATGKTENISASNEGYDKSPAF
                     SPDGSRMMWLSMQTPTYEADRNRIMIYDFATKKTMEATAGFDYTVDGAKWAPDGHRIY
                     FISGINATDQLWVYDGRLRSYPPIKQITSDIGDYTSFSVSDNKSDPSIIATMMSISAP
                     TEIFRVDVKTGTSKQITYTNKNVLSTVKMGKVEKRMIKATDGKEILTWVIYPPDFDAS
                     KKYPTLLYCQGGPQSTVSQFFSYRWNFQLMAANGYIVVAPNRRGLPSFGTAWNREISG
                     DWGGQCMRDYLSAIDEVSKEPFVNKDRLGAVGASFGGYSVYWLAGHHQKRFKTFISHC
                     GVYNLESMYGTTEEVWFPDFDLGGSFWQTPKPKSYTQFSPVDFVQNWDTPILVIHNEK
                     DFRVPLSQGMEAFSGARLQNIPARFLYFPDEGHWVNKPQNSVMWQRVFFDWLDRYLK"
     gene            complement(2954..3709)
                     /gene="kdsB"
                     /locus_tag="PKY09_12170"
     CDS             complement(2954..3709)
                     /gene="kdsB"
                     /locus_tag="PKY09_12170"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNB13649.1"
                     /translation="MTLKVLAVIPARFASTRFPGKPLALIKSKTMLQRVYEQAKKATA
                     VNQIIIATDHDDIANHARQFGAEVMMTRQDHASGTDRIAEVVSRLTTTFDVIVNIQGD
                     EPFIQPKQINQLAEVFSDNSIDIATMIKPVSGMQEYESPDVVKVVIAENGNALYFSRW
                     PIPFFRKEKNEKKICDTAYKHLGMYAYRTSIIKKIAILPVSVLEEAEQLEQLRWLQNG
                     YTIRTIVTDTETIAVDTPADLIKAENYLERKTN"
     gene            complement(3706..4140)
                     /locus_tag="PKY09_12175"
     CDS             complement(3706..4140)
                     /locus_tag="PKY09_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13650.1"
                     /translation="MKNTYYFIALFTFISIQALSQKINPTTEAEYLYGSVGYKIQLNN
                     KLPVKEGYTLRDFKPVVEENRQVEFKGVYRKGENKPCAIIMIYTKLRNTPQYYCIPTD
                     DADAALWRKFNDSLLDDSENQQPQLQFFAYCIAFLASQTQQQ"
BASE COUNT         1263 a          830 c          770 g         1389 t
ORIGIN      
        1 ttacaacaaa ggaagaactt caagatcaag ctccctcatc tgcccttgca agtctgtttt
       61 ttggtttcac aaattatctt ttagcaagta tcattttaat taatccgttt aagtatattc
      121 catataacct acctgtattt ggaaccattt ttattggagt taatgcccta ttttattttg
      181 caaatagaaa actacttgtg aaggccaaaa aatacattga aattgaacac tactatgaca
      241 agaataataa gttaaaaaaa atacacttta tgcttataac cactttttac atgctagggt
      301 ctattgttac aatgatattg gcaggaataa agtatgcaac atcgaattaa taatacaatc
      361 tcccccctca tcctcgtctg aagggtagca ttgtgtgcga gagacgagga tgtaattaac
      421 gtttgtcata gaatacaaaa aacaaaactt gaaataattt tttacttttg agagtataat
      481 catataaaca aagtgtatgt cgggcaatag gtatttaata cgcaaccaga atgctgttta
      541 ttttttaaca tttacaattg ttaaattgaa caaaaacaag attctgaaag cagacgtgaa
      601 ttattattgg cacaatttcg tgatcggcta aagattatgg cgaagagaaa ggattggtta
      661 aggtcagttt gttgtaagga tatgaggtgt acttttgcat cctcgtccct tacgcacgta
      721 cctgacttca gacgaggacg agtggggtta taatttagaa agtattgatt acgtagattt
      781 aagattttat tagcaacaaa aaaaggtgaa tccgattgga ttcacctttt tttaaatatc
      841 tgatagccgt tttttatttc aggtatctgt cgagccagtc aaaaaacaca cgctgccaca
      901 taacactatt ttgtggtttg ttaacccaat gaccttcgtc agggaaatat aaaaatcttg
      961 caggaatatt ttgtaaccgt gctcctgaaa atgcttccat gccctggctc aaaggcactc
     1021 tgaaatcttt ttcgttatga atgacaagaa ttggagtatc ccagttctgc acaaagtcaa
     1081 caggagaaaa ctgtgtgtat gattttggct taggtgtctg ccagaaggag cctcccagat
     1141 caaaatcagg aaaccacact tcctctgttg tgccatacat actttctaga ttataaacac
     1201 cgcaatgcga aataaatgtt ttaaaacgtt tttgatgatg tcctgcaagc cagtaaacac
     1261 tgtagccgcc aaagctggca ccaacagcac ctaaacggtc tttgtttaca aaaggctctt
     1321 tgcttacttc gtcaatggca gacaggtagt cgcgcataca ctgcccaccc cagtcaccgg
     1381 atatttcgcg gttccatgct gtaccgaatg atggtagccc tcttcggttt ggtgcaacca
     1441 caatatagcc attggcagcc atcaactgaa aattccaacg ataactaaaa aactgtgaaa
     1501 cggtactttg tggcccaccc tgacagtaaa gtaaagtcgg atattttttt gatgcatcaa
     1561 aatcgggagg ataaattacc caggtcagaa tttcttttcc gtcagtggct tttatcattc
     1621 gtttttctac cttacccatc tttacagtac tcaacacatt tttattggtg tatgtaattt
     1681 gttttgaagt tcctgtcttt acatcaacac gaaatatttc agtaggtgca gaaatagaca
     1741 tcatggttgc aattattgat gggtcgctct tattgtctga aacagaaaac gaagtgtaat
     1801 caccaatgtc agaagttatt tgtttgatag gagggtagct gcgtaaacga ccatcataca
     1861 cccacagttg atctgttgca ttgataccac tgataaaata aattctatgt ccatccggtg
     1921 cccattttgc tccgtcaacc gtatagtcaa atccggcagt ggcctccatc gtttttttag
     1981 ttgcaaaatc atagatcatg attctgttgc gatcggcttc atacgttggt gtttgcatgc
     2041 taagccacat cattctgctg ccatcaggcg aaaatgcagg cgacttatca taaccttcat
     2101 ttgacgcaga aatattttct gtttttcctg tagctacatc ataaagataa atgtcagagt
     2161 ttgtgcttac ggcataatct tttcctgcca attttttgca tgtgtaggca atcttatttc
     2221 cgtctgcact ccaggcaatc tgttcaccac cgtcattagg tttcattggt gtgtcaaaac
     2281 gttcgccttt ttgtatgtct ttcagttcac caactgcatc attttcaaaa tcggcaataa
     2341 atacgtgcga gtaagaacca tcttcccatt ctgtccagtg gcgatacatc aaatcatcat
     2401 aaatttttcc ggtggctttg ggtaaatcgg gataaagatc ctgagtagtt ttatcaacct
     2461 ttacttcttt agccatccat attctgtttc ctgcttttga aataccaaaa cacgaaatat
     2521 cgtcagcagt tgtagtgatt tgtttttctc ctgtgccatc agcattgata caccacaagt
     2581 tatttgcacc acctttatcg tttaggaaat atattttttt gctgtctgca ctccatgccg
     2641 gtgatgattc gttgtcagca ctgccagcaa taagcttagg ctcaccacct gtaacagcaa
     2701 cagtgtacat atccgtattg ccgttgtttc ccgacagatt aaaatttctg acagtgtaaa
     2761 caacagtttt gccgtcaggt gaaagaacag catcacccac acgacctaat ttccaaagta
     2821 gctccggagt aagtttgttt tgtgcaaacc ctgctgatgc taaagtcaga aagcctgcta
     2881 acaagattga ttttctcatt gtattatatg attataaatt tcagtgtact aaagtagcgt
     2941 attgaaaaaa cattcagttt gtttttcttt caagataatt ttctgcttta atcaaatcgg
     3001 ctggagtgtc cacagcaata gtttctgtgt cagtaactat tgttcttatg gtatatccgt
     3061 tctgtaacca gcgaagttgt tcaagttgtt ctgcttcttc cagcactgat acaggtaaaa
     3121 ttgcaatttt ttttataatg gatgtgcgat aagcatacat gcccaaatgt ttgtatgctg
     3181 tgtcacaaat ttttttctcg ttcttttctt ttctgaaaaa aggtattggc cagcgactaa
     3241 aataaagagc attaccattt tcggcaatta caacttttac tacatcaggg ctttcatatt
     3301 cctgcatacc tgaaacaggt tttatcatgg tggcaatatc aatactgttg tcgctgaaaa
     3361 cttctgcaag ttgatttatt tgtttaggct gaataaaagg ttcatcaccc tgtatattta
     3421 caatgacatc aaatgttgtt gtcagcctac tgacaacttc ggcaatgcgg tcagtgccgg
     3481 atgcatgatc ttgtcttgtc atcatcacct ctgcaccaaa ctgtctggca tgattggcaa
     3541 tatcatcatg gtctgtagca atgataatct gattaacagc agttgctttt tttgcttgtt
     3601 catacacacg ctgcaacatg gttttgcttt ttatcaatgc aagtggctta cccggaaaac
     3661 gggtagatgc aaaacgtgca ggaataactg ccaaaacttt caatgtcatt gttgctgggt
     3721 ttgtgaagca agaaatgcaa tacagtaagc aaaaaactgt aattgtggtt gctggttttc
     3781 agaatcgtct aacaggctgt cattaaattt ccgccataat gcagcatcgg catcatctgt
     3841 aggaatgcaa taatattgag gagtatttcg cagtttggta taaatcataa taatggcgca
     3901 aggtttgttt tcacctttac gataaacacc tttaaattct acctgtctgt tttcttcaac
     3961 aacgggttta aaatcacgta acgtataacc ttcttttacc ggcagcttgt tattgagttg
     4021 tattttatat ccaacagagc catataaata ttcggcttct gtagttgggt ttattttttg
     4081 tgataatgcc tgaatactta taaaagtaaa caaggcaata aaatagtatg tatttttcat
     4141 cacctcaaaa gtaaagtttc atttttaaaa taacacaagt atataaaaaa cgaaagggaa
     4201 ctccgttgag ttccctgttc gtaacctaaa actaacccta tgaaaaaaat at
//
LOCUS       DAOQNP010000134         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_9312_length_4237_cov_5.138690, whole genome shotgun sequence.
ACCESSION   DAOQNP010000134 DAOQNP010000000
VERSION     DAOQNP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9312_length_4237_cov_5.138690"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKY09_12180"
     CDS             <1..550
                     /locus_tag="PKY09_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13651.1"
                     /translation="VMYRFFPHKTGTEIFSIAVLKKPDLNDLDSERVAAKSLKFADKV
                     QTDFVKKHIDRGEEMFYFIHDNHVRFMPKHLKNELGQLLNLPVIHAGTAAFEIKGKNL
                     VPSFELALSTAISQEKFSKAEVDKPAMLKLLKGETRLMDSLPIGQHLLTFQNIPLMFL
                     KKIEGRVNVNYPRSWFIRMNIE"
     gene            622..2730
                     /locus_tag="PKY09_12185"
     CDS             622..2730
                     /locus_tag="PKY09_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNB13652.1"
                     /translation="MKKKTTVAKQESQLKAQSGIKLSQLNKYLGLLLAAIAFILYANT
                     LNHDYTVDDGTVMQNNKIVKKGVSAIPEIFTTAYRKGFWDRNESLYRPLSVAMFAVEW
                     QLAPENPAIGHWVNVLLYVLTAWLLFRFLVNLFKDDWLFAFVTTLIYVIHPIHTEVVA
                     NIKSRDEILCFLFLILSFNSFLNLLDKAKPVYALGVLFYFILSLLSKESSVTAIIIFP
                     LIAYFFRQHTVGSSIKLSWSPAIAIVVYLSLRFVVLKGISNFNEILPINNSIVDAQNY
                     FQQLATAVMIAGKYLYLLIIPYPLSFDYSYNTFPVVDFSNIKAFFSLAVYVFLLIMAI
                     KGFKSRSRFSFGIFYFLLTMSIVSNVFFLIESVMAERFTYLPSLGICIALTSVLFTLF
                     KVSSVVRDKIDINKIISANRMFFGLILIVFVVYGGMTVARNAKWKSNLTLLATDVKTC
                     PESARIRYAYGSAILIEQALKEKDHSKKMNLLDKSIEQLEKGVAILPTYSEAYYHLGL
                     AYKERENYDKAIESFEKASKSKTFTDPQFFISWGVACGKAKRYQESIKALNHAIDLDP
                     AAKEAYLNLGVFYDEMQESDKAETSLMKAIKLDSLSDGAYYNLGNVFAHRNNFVKAIQ
                     NYKKALEINPANEDAVNNTGNSYAAMQDYENAIIYFRKAIELNPANTRALNNLGVTLM
                     LTGKKEEGEQYIAKARAIGN"
     gene            complement(3111..>4237)
                     /locus_tag="PKY09_12190"
     CDS             complement(3111..>4237)
                     /locus_tag="PKY09_12190"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HNB13653.1"
                     /translation="GKADPDTVTPLANSMMLVLLGSHLSPPLFHVEHISEYASTLQKA
                     NSFDQVNIDTIEHFDKVYEEYKAKTDTLFRGQREAKWRLYSKLQRQWITEKLFEKKDS
                     YQKLIENLVEIGEADFIIQIQELLSAHHIDTANSISILGYLQHHSCPTPLLDWTYKFE
                     NAVYFALDGLTDNPGTVEIEDYCSVYYIEEKHFEGGNLRKIIEDGLDSLEQPMLMKEI
                     AKIANGNENKRIAMEKHFAGRKLFDRTKIAGSGLISHMTKIEHLINFPIGYFSDKDVA
                     SGILFSLNNSKNILNQSGVFTWNADQSKPIEMVGDEQFPDDKTDEKNKNYRFCNCFNI
                     HKKIADHVRQRLEADGITKEFIYPTPDINTWDVFEKAKAE"
BASE COUNT         1272 a          680 c          816 g         1469 t
ORIGIN      
        1 tgttatgtac cgcttttttc cacataaaac cggaacagag attttttcaa tagcagtatt
       61 aaaaaagcct gatttgaatg acttagattc tgaaagagta gctgcaaaat ctctgaagtt
      121 tgctgataaa gtacagacag attttgtgaa aaaacatatt gacaggggag aggagatgtt
      181 ttattttata catgataatc atgtcaggtt tatgcctaag catttaaaaa atgaattggg
      241 tcagttgtta aatcttccgg taatacatgc tggtacagca gcatttgaaa taaagggcaa
      301 aaatttagtg ccgtcatttg aacttgcctt atcaacagca ataagtcaag aaaaattcag
      361 caaggcagaa gttgacaaac ctgccatgtt aaaactgctc aaaggtgaaa cgcgtttaat
      421 ggactcttta ccaataggcc agcatttact aacatttcaa aacataccat tgatgttttt
      481 aaagaaaata gaagggcgtg ttaatgttaa ctaccctcgt tcatggttta taagaatgaa
      541 cattgaatag gttgaaaagt gctttataaa tagcactgta ttttttgtaa tttcacttta
      601 ctttgtttca aacatcacgt gatgaaaaaa aagacaacgg ttgcaaaaca ggaaagtcag
      661 ttaaaagcac agtccggcat taagttatca cagttaaata aatatcttgg tttgttgctt
      721 gctgcaatag catttatcct ctatgccaat acgctcaatc acgattacac tgttgatgat
      781 ggcactgtga tgcaaaacaa taaaatagtg aagaagggtg taagtgctat tcctgaaatc
      841 tttacaactg cataccgaaa gggtttttgg gacagaaatg aaagtttgta tcgcccatta
      901 tcggttgcaa tgtttgctgt tgaatggcaa ttagctcctg aaaatcctgc tattggtcat
      961 tgggttaatg tgttgctcta tgtgctcaca gcatggttgt tgtttcgttt tctggttaat
     1021 ctttttaaag acgactggct gtttgccttt gtcaccactc ttatctatgt cattcatccc
     1081 attcatacag aagtagtagc caatataaaa agcagagacg aaattctatg tttcttattt
     1141 ctgattcttt cattcaatag ctttttaaat ttactggata aggcaaagcc tgtttatgcc
     1201 ttaggcgttt tgttttattt tattttatca ctactgtcaa aagaaagttc tgtcacagca
     1261 ataatcatat ttccattgat tgcatatttt ttcagacagc atacagtagg tagcagcatt
     1321 aaattgtcgt ggtcaccggc aattgctatt gtagtctatc tgtcattgcg ttttgttgtt
     1381 cttaaaggaa tctcaaactt taatgagata ttgcctatca acaattcaat tgttgatgca
     1441 caaaattatt ttcagcaatt ggcaacagca gttatgattg ccggaaagta tttgtattta
     1501 ctcatcattc cttatccgct gtcatttgat tattcctata acacttttcc tgttgtagat
     1561 ttttctaata taaaggcatt tttttcgttg gcagtctatg tctttttact gattatggct
     1621 attaaaggat ttaagtcgcg cagcaggttt tctttcggta ttttttattt tctgcttacc
     1681 atgagtattg taagcaatgt gttttttttg attgaatctg taatggcaga gcgttttacc
     1741 tatcttcctt ctttaggcat ttgtattgca ttaacatcag ttctttttac gctatttaaa
     1801 gtcagtagtg ttgtcagaga taaaattgat attaataaaa ttatatcagc aaatagaatg
     1861 ttttttggtc tgatattaat tgtttttgtt gtttatggcg gaatgacagt tgcaagaaat
     1921 gcaaaatgga aaagcaatct gactttactg gctactgatg taaagacgtg tcctgaaagt
     1981 gcacgaataa gatatgctta tggcagtgcc attcttattg aacaggcttt gaaagaaaaa
     2041 gatcattcaa aaaagatgaa tcttcttgat aagagtattg agcagttaga aaagggagtt
     2101 gctattttac caacgtattc tgaggcatat tatcatcttg gacttgcata taaggagcga
     2161 gaaaattatg ataaggctat tgaaagtttt gagaaggcat ctaagagtaa aacatttacc
     2221 gatccacagt tttttatttc atggggtgta gcatgcggaa aagcaaaacg atatcaggaa
     2281 agtataaagg cattgaacca tgccatagac cttgacccgg cagctaagga ggcatatctt
     2341 aatttgggtg tgttttatga tgaaatgcag gagtctgata aagccgaaac gtctttgatg
     2401 aaagcaatta aactggattc gctttctgac ggagcttatt ataatttggg aaatgttttt
     2461 gcccacagaa ataatttcgt taaggcaatt cagaattata aaaaagcatt ggaaattaac
     2521 cccgcaaatg aggatgcagt aaataataca ggaaacagtt atgccgctat gcaggattat
     2581 gaaaatgcaa taatttattt cagaaaggca attgaattga atcctgctaa tacaagagca
     2641 ttaaataatt taggtgttac actgatgtta actggcaaga aagaagaagg ggagcagtat
     2701 attgcaaagg ccagggcgat tgggaattaa acggttgtgt ttgtagagga gtaccaatgt
     2761 ttagtacata ttgcatagtg tggcaacaaa tgtttgcatt tgagaagtcc aagcagtact
     2821 tgaatgaatt ctgccccacc cacgcgtttt cttaaaaaaa tgaaagtaat attaaaagta
     2881 aatatgggta tattttggta attacttgtt ggacctgcga atctcatcta tatgtgaaac
     2941 gttaagtacg caactaagat tgaatgattc attatcattt aatcgaaagt tgggagcgta
     3001 gctcgcgtaa gcttagtggt gtgagataca gatagctatc ggtatcttcc cttcagcacc
     3061 cgctggcggg gcgggctact cgattacctg ctgccctttt agtttttttg tcattcggct
     3121 tttgcttttt caaaaacgtc ccaagtgttt atgtcaggtg ttgggtaaat aaattctttt
     3181 gttattccat ctgcttctaa tctttggcga acgtggtcag caattttttt atgaatgttg
     3241 aagcaattac aaaatctata atttttattt ttttcatcgg tcttatcgtc agggaattgt
     3301 tcgtcaccaa ccatttcaat aggtttagat tggtctgcgt tccatgtaaa tacaccagat
     3361 tgatttaaaa tatttttact gttgttgagt gaaaacaaaa ttcctgacgc aacatctttg
     3421 tcactaaagt atccaattgg aaagttaatt agatgttcaa tcttggtcat gtgggagatg
     3481 agtcctgaac ctgcaatttt tgtgcggtca aataattttc tgccagcaaa atgtttttcc
     3541 attgcgattc ttttattttc gttaccgttc gcaatttttg caatctcttt cattaacatc
     3601 ggttgttcga gtgagtccaa tccgtcttcg attatctttc tcaaattgcc gccttcaaaa
     3661 tgtttctctt caatgtaata aacactgcaa taatcttcaa tctctacggt tccagggtta
     3721 tcggttagac catccaatgc aaagtaaact gcattttcaa atttatatgt ccagtctaaa
     3781 agaggtgttg ggcaactatg atgttgtaag taacctaaaa tagagattga atttgctgtg
     3841 tcaatatgat gagcactaag caactcttgt atttgaataa tgaaatctgc ttctccaatt
     3901 tcaacaaggt tttcaattaa cttttgataa ctgtcctttt tttcaaatag tttttcagtt
     3961 atccattgtc gttgtagttt actgtacaat ctccattttg cttctctttg ccctctgaaa
     4021 agtgtgtctg tttttgcttt gtattcctca tacactttat caaaatgttc tattgtgtca
     4081 atgttgactt ggtcaaagct gtttgctttt tgtaatgtgg atgcgtattc agaaatatgt
     4141 tcgacatgaa acagtggtgg gcttaagtgc gaacccaata aaacaagcat cattgagttt
     4201 gccagtggtg taactgtatc ggggtctgct tttccgt
//
LOCUS       DAOQNP010000135         4173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_9556_length_4173_cov_9.324915, whole genome shotgun sequence.
ACCESSION   DAOQNP010000135 DAOQNP010000000
VERSION     DAOQNP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_4173_cov_9.324915"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..>4173)
                     /locus_tag="PKY09_12195"
     CDS             complement(37..>4173)
                     /locus_tag="PKY09_12195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13654.1"
                     /translation="LKVAGSNVFLTKNIYVIYGGGCFNQCNLVNGDFEQFNQQECPYT
                     GQSGMVFTAWPSGVNNIVDCWSLVPPGTCDFYTQACNSTTALGNGFGGSLPHSADSYI
                     GLIPYVGTPTGNLTQYREYFTQQLSFSIRPNCDYHVGVYAKIASNGIAGTTGYGDVGL
                     PLQVQLTDLFPNQFVGSTFLLDINSGLTSSFTPAISSNTNWQLIETTFNSGIVTGTKF
                     LTVGNYYNNTNTQLTDPLKNDQQIYIFLDSLFIEFVDFTSTISDVTCVQNGSINLTSV
                     CGTAPFTYQWSNGATTQNITNLSSGNYTVTITDAKGLHFTDSYNVPNTSCTPQLSATV
                     NNTCSGSCNGQIDLTVSGNCNGTFTYLWSNGATTEDIIGLCAGTYTVTVTAQNGCTAT
                     ANYTVTAETAPIISFDYLPLQCQGTYSLQNPTVGIDYYWEFRDNAGNLIFSNTGSGGL
                     TSVTLPPGASPSQLIISADNNGCKVSKTFLLGECCPPDPNTDDFAVYGNITLSQLVVL
                     LQANRPSWVNDNTINGQPQQGYCVNIIPSKLVYYKLNTGLTVNTTNNLFIYGNLTIDK
                     SIDLSEFSLVMAADREVIVNNNVSVTLHSDIGVFFNTCGKYMWRGIRLLGSNSKIYAR
                     GQTSHLSTYYNNQINDAVNGINSSANGKFYLSNTLFNRNLNGIFITNSNATINNSYIT
                     DCFFTSKDNALINDQVLLENPINNTTATRSRTGISLTRLNNTVIGFDEALDINGTYAN
                     TACFLGDQFNKFENIDYGIWLQRCAGIEVYRNEFKRIIKGIRTSGDASNAASISVGKA
                     MGVNLSHNNIFDQFTENGIELTGNASIAANNNFFYNTTLISGSSDKLRSDIEFNNAYS
                     LTKNSIMNNEFSHYYYAAVYLHNNTSGSMSVDVIGNKFYGRLNPTNGNPWSVYGIYAD
                     AAPANKYVMNINKNLVIDDTRYGIRIRNNDNANIVGNILTYHMASNVTFNNNTQWIRG
                     IFLSNSSLNKVEMNHILWSNYPAGFSITYPDKLQGISMENVTNSNIICNKMDGMGAGV
                     YIQNNNIGTSYITNITKNCYNGYKLNNASIDPVGNNTTSNNNTFSNITSFRVTGTSGT
                     PSQNWYYYNSNDMPVPNNNVVAAVQVPLFLQPCTSFTDEPDSSQMRTETEMLQQVINN
                     ETEFAQFEEENKYLTEEAALKLLKADSLLRDTLPNTENFIQANETLGKGKYIEAEKAL
                     AEGNTGNATAIMATITDNNLMEENKRAVMGMIIKMNDSIPLTAADTAALESIAYTPAI
                     VGGTAVYIARGILAIEVDDEWLTSSYRMANDNQKEKNKNTLDIYPNPARNFIFIKCTG
                     SITYEITDAAGKQYKQGNFDLQGNAVVSINIEELMQGVYIVKATQENCTKVAQIVVIK
                     "
BASE COUNT         1228 a          805 c          744 g         1396 t
ORIGIN      
        1 ttcttaacat aaaaacagtt ttaatacgtt ctgaaattat tttataacaa caatctgcgc
       61 aaccttagtg caattttcct gtgtagcttt tactatgtaa actccctgca taagctcctc
      121 aatgttaatg cttactactg cattgccctg caaatcaaaa ttaccctgct tatattgttt
      181 ccctgccgca tccgttattt catacgttat gctgcccgtg cattttataa aaataaaatt
      241 tctggcaggg ttgggataaa tatccagtgt atttttattc ttttcttttt ggttgtcatt
      301 agccattctg taagatgatg tcagccattc atcatccacc tcaatggcta aaatacctcg
      361 tgcaatatac acagctgttc cacccactat agcaggcgtg taagcaatag attcaagcgc
      421 agcggtgtca gccgcagtaa gcggaatgct gtcattcatt tttataatca ttcccattac
      481 cgcccgcttg ttttcttcca tcaggttatt atctgttatg gttgccatta tggctgtagc
      541 attaccggtg ttgccctctg ccagtgcttt ttcggcttca atatattttc ctttgccaag
      601 cgtttcattt gcctgtataa aattttctgt attaggcagt gtatctctca gcaggctgtc
      661 ggctttcagc aacttcagtg cggcttcttc tgtaaggtat ttgttttcct cttcaaactg
      721 tgcaaattcc gtttcgttgt ttattacctg ctgaagcatt tccgtttccg tacgcatctg
      781 gctgctgtca ggctcatcag taaagctcgt acagggttgt aaaaataaag gaacttgaac
      841 agcagcaaca acattattgt taggcacggg catatcatta ctgttgtagt aataccagtt
      901 ttgcgatgga gtaccgcttg tacccgtaac tctaaagctt gtaatgtttg aaaatgtatt
      961 gttgttactt gttgtattat ttcccacagg gtcaatggag gcattgttta gtttatagcc
     1021 gttgtagcag tttttggtaa tgtttgtaat atagcttgtg ccaatgttgt tgttttgaat
     1081 ataaacacct gcacccatgc catccatttt attacatata atattgctgt tggtcacatt
     1141 ctccatacta attccctgca atttatcggg atatgtaatg ctaaaaccgg caggataatt
     1201 actccaaaga atgtgattca tttcaacctt attcaatgat gagttactta gaaatattcc
     1261 cctaatccat tgtgtattgt tattaaaagt tacattggaa gccatgtggt aagtgagaat
     1321 attaccaaca atgttggcgt tgtcgttatt gcgtattcta ataccatagc gcgtatcgtc
     1381 aatcacaagg tttttattaa tgttcatcac gtatttgttg gcaggggcag catctgcata
     1441 aatgccgtaa acagaccagg ggtttccatt tgtcggatta agtcttccat agaacttatt
     1501 ccctatcacg tcaacactca tactacctga ggtattatta tgcagataaa cagcagcata
     1561 atagtaatgg ctaaattcat tattcattat actgttttta gtaagtgaat atgcattatt
     1621 aaattcaata tccgaacgta atttgtcgct acttccacta attaaagtgg tgttataaaa
     1681 aaagttatta tttgcagcta ttgaagcatt tccggtcaac tcaattccat tctctgtaaa
     1741 ttggtcaaaa atattattat gtgatagatt aactcccata gccttaccta cggaaattga
     1801 agctgcattg cttgcatcgc cactagtgcg tattccctta ataatccgtt taaattcatt
     1861 gcggtacact tcaataccgg cacatctttg cagccaaatc ccataatcaa tattctcaaa
     1921 tttattaaac tgatcaccta aaaaacaagc agtattagca taggtgccgt taatatccag
     1981 tgcttcgtca aagcctataa ctgtattatt gagccttgtt aggctaattc ctgtcctgct
     2041 tcgagttgca gtggtattat tgattgggtt ctccagcaat acctgatcgt tgatgagggc
     2101 attatccttg ctggtaaaaa aacaatcagt aatataggaa ttgttgattg ttgcattact
     2161 atttgtaata aaaattccgt tcaagttgcg attaaataga gtattactta aatagaattt
     2221 accgtttgcc gaactattta ttccgttgac agcatcattt atttggttat tgtaataagt
     2281 ggaaagatgt gaagtttgtc ctctagcata tattttgcta ttgctaccca ataatctaat
     2341 tcctctccac atgtacttac cgcaagtatt aaaaaacaca ccaatatcgg aatgaagcgt
     2401 aacacttaca ttattattta caattacctc tctatctgca gccataacta atgaaaattc
     2461 gcttaggtca atactcttat ctattgtcag gttcccataa ataaacagat tgtttgtagt
     2521 atttacagtt agtcctgtat tcaatttgta gtaaaccaac ttacttggaa ttatattaac
     2581 acaataacct tgttgtggtt gtccattaat tgtattgtca ttgacccatg aaggtctatt
     2641 ggcttgcagc aaaactacca attgacttag tgtaatgtta ccgtaaacag caaaatcatc
     2701 tgtattcgga tcaggagggc aacattcacc tagtaaaaat gtttttgaaa ccttgcaccc
     2761 attgttgtct gcactaataa ttaactgtga tggtgaggct cctggtggta gcgtaacaga
     2821 agtaagacca ccgcttccag tattggagaa tataagattg ccggcattat ctctaaattc
     2881 ccaataataa tcaataccga cagttggatt ttgcagacta taagttcctt gacactgtaa
     2941 cggtaaataa tcaaatgaaa ttataggagc cgtttctgca gtaactgtgt agtttgcggt
     3001 tgcagtacac ccattttggg ctgttacggt aacagtatat gttcctgcac aaagccctat
     3061 aatatcttcg gttgttgctc cgttactcca taagtaagta aaagttccgt tacagtttcc
     3121 cgaaacagtt aaatcaattt gtccgttaca ggaacccgag catgtattgt ttacagtggc
     3181 ggataattga ggcgtgcaag aagtgttagg tacattgtaa ctatccgtaa aatgcaatcc
     3241 tttagcatca gtaatggtaa cagtataatt tccagatgac aaattggtta tgttttgagt
     3301 tgtagctccg tttgaccatt gataagtaaa gggcgcggta ccacacacag aggttaaatt
     3361 aatagacccg ttttgaacac aggtaacatc agaaatagtt gaggtaaaat ctacaaattc
     3421 tataaataac gaatctaaaa aaatgtaaat ctgctggtca ttttttaaag ggtctgttaa
     3481 ttgtgtattc gtattattgt aataattgcc aacagtcaga aattttgttc ctgttactat
     3541 tcccgaattg aaagtggttt caattaattg ccagtttgta ttagatgaaa ttgccggagt
     3601 aaaagatgag gtcagtccgc tgtttatatc caataaaaag gtagaaccca caaattgatt
     3661 aggaaataag tccgttagtt gaacttgtaa tggtaatccc acatccccat atcctgtagt
     3721 tcctgctatg ccgttagacg caatttttgc ataaactccg acatgataat cgcaattagg
     3781 gcgaattgaa aaagataatt gttgggtaaa atattccctg tattgtgtta aattgcctgt
     3841 tggcgttccc acataaggta ttaatccgat gtaagaatca gcgctatgtg gcaacgagcc
     3901 tccgaaacca ttacctaatg ccgttgtgga gttgcatgcc tgtgtataaa aatcacaagt
     3961 tccgggcggc acaagactcc aacaatcaac aatgttattt acaccagaag gccaagctgt
     4021 aaaaaccata ccactctgac ctgtataggg gcattcctgc tggttaaatt gttcaaaatc
     4081 accattcacc aaattacatt gattaaaaca gccgcctccg taaattacat atatattttt
     4141 tgtaagaaaa acatttgaac ctgcaacctt tag
//
LOCUS       DAOQNP010000136         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_9802_length_4111_cov_9.030572, whole genome shotgun sequence.
ACCESSION   DAOQNP010000136 DAOQNP010000000
VERSION     DAOQNP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9802_length_4111_cov_9.030572"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKY09_12200"
     CDS             complement(<1..775)
                     /locus_tag="PKY09_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13655.1"
                     /translation="MKKGLLITLALGAAATLNAQEATKKTNKLEAIDNGVKLNVSQRV
                     HGVSGTTFTAGCENTLGSSANSYTALGGGRSNLIYNPAINTIAFIHRGNPSGGVGTSS
                     GDLFWDISTDGGATWNINQGPAYQPVNSNNARYPLGSIYNPAGNTDPANAWVAGFGAN
                     TNGSGWISQAHCTSKLDGTNANQETQDFVLGGFMGDIPNSMETDNVGNVWVSDFQDDA
                     IGSQAYANTNLWLTKGVWNGAANKYDYTNIAMPFVADLDP"
     gene            1005..1080
                     /locus_tag="PKY09_12205"
     tRNA            1005..1080
                     /locus_tag="PKY09_12205"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:His,seq:gtg)
     gene            1156..1767
                     /locus_tag="PKY09_12210"
     CDS             1156..1767
                     /locus_tag="PKY09_12210"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNB13656.1"
                     /translation="MEYNTKRPELIISEYGRNIQKMIDHAIQLESQEERTKAANAIIN
                     AMVVLNPQIREYNDYKIKLWDHLFIMSDYKLQCDSPFPMPSPEKRKLTPEKVPYPTYN
                     IKFKHYGSIIYNMIEKTCEMEDGPQKEQVILMIANFMKQSYVNYNRDNVNDEVIFEHL
                     RKISNGKLSLKEEVRLRYIADVRPMNNNGGGKKKKKKKKSSQH"
     gene            1771..3078
                     /gene="murA"
                     /locus_tag="PKY09_12215"
     CDS             1771..3078
                     /gene="murA"
                     /locus_tag="PKY09_12215"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNB13657.1"
                     /translation="MANSFEITGGQQLHGEIEPQGAKNEALQILCAVLLTDEKVTIHN
                     IPDIRDVNKLIDLLRSLGVIIEKLKHGSYTFQSDNINLDFLKTDDFIRQAGSLRGSIM
                     IAGPLLSRFGIACVPKPGGDKIGRRRLDTHFIGFQNLGAKFDFDSHTKIFSVSAKKLK
                     GAYMLLDEASVTGTANIIMAAALAEGTTTIYNAACEPYIQQLCKMLVSMGAKISGIGS
                     NLLSITGVSKMTGCTHTMLPDMIEVGSFIGLAAMTQSEITIKNCSVPNLGIIPSTFQR
                     LGIKLEIKGDDIYVPAQKNYEIDTFIDGSILTIADAIWPGLTPDLLSVILVTATQANG
                     TALIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLNRKHSLKGIEMTSPDIRAG
                     VALLIAALSAKGKSIIHNIEQIDRGYERIDERLIKLGAHIKRI"
     gene            3169..3699
                     /locus_tag="PKY09_12220"
     CDS             3169..3699
                     /locus_tag="PKY09_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNB13658.1"
                     /translation="MTTTLKATFLSLAIIGTAFFSQAQKPIEGIAIGNKAPELAFTSP
                     EGKTIALSSLKGKIVLIDFWASWCGPCRMENPNVVAAYNKYKDKKFKGAKGFTVYGVS
                     LDQDKNRWMNAIKQDKLDWPSHVSDLAGWNSKAAQIYRVNSIPANWLIDGNGYIVGSN
                     LRGQALDAAIEKLLAQ"
BASE COUNT         1322 a          753 c          805 g         1231 t
ORIGIN      
        1 taggatctaa atcggctaca aacggcattg caatgttggt gtagtcatat ttattggcag
       61 caccattcca cacaccttta gtcaaccaaa ggtttgtatt tgcataagct tgtgaaccta
      121 tagcatcatc ctgaaaatca gaaacccaaa cgttaccaac gttatctgtc tccatgctgt
      181 ttggaatatc acccatgaaa ccacccagaa caaagtcctg agtctcctga ttggcattgg
      241 taccatctaa tttagaagta caatgtgcct gactgatcca tccgctaccg ttggtgtttg
      301 caccaaaacc cgcaacccat gcattagcag gatctgtgtt tcctgcaggg ttatagatgg
      361 agcctaatgg ataacgtgca ttgttggaat ttacaggctg ataagccgga ccctgattga
      421 tgttccaagt agcaccgcca tcagtagata tatcccagaa aagatctccg gaacttgtac
      481 ctacaccacc ggaaggatta ccacggtgaa tgaatgctat tgtgttgatt gcagggttgt
      541 atatcagatt ggaacgacct ccaccaagtg ctgtataact attggcagaa cttcccaaag
      601 tgttttcgca tcctgcagta aatgttgtgc ctgatacgcc atgcacacgc tgagaaacat
      661 taagcttcac accattatca atagcttcaa gcttatttgt ttttttcgtt gcttcctgag
      721 cattcagcgt agctgcagca ccaagagcaa gagtaattag taaacctttt ttcattgttt
      781 ataaaattta atttttacat gattaatcag acaaaggtag caactttttt tgttttcaaa
      841 atttcaggaa ctatttattc tgattattta tttcatcttt actgatatca gacaatcttc
      901 acataaaaaa acatattcaa aatatcattt gtgcgaatgt ttttttgcac tatttttgca
      961 cctcgttaaa gaaggatatg tttttaattc ttcttttttg atatggtgaa tgtagctcag
     1021 ctggttagag catcagattg tggttctgag ggtcgtgggt tcgagcccca tctttcaccc
     1081 tgatttttta aattgaagtt ttaagtaaaa ttttaacatg aaaattttac acttcggtaa
     1141 ttagccttaa attttatgga gtataacacc aaacgtcctg agttgattat ttcggaatat
     1201 ggacgcaaca ttcaaaaaat gattgaccat gccattcaac tcgaaagtca ggaagaaagg
     1261 acaaaagcgg caaatgcaat tattaatgca atggttgtgc tgaatccgca aatcagagaa
     1321 tataatgatt acaaaataaa actttgggat catctgttta ttatgtcaga ttacaaactg
     1381 caatgcgatt cgccttttcc gatgccatcg ccagagaaaa gaaaacttac tcctgaaaaa
     1441 gtaccatacc ctacttacaa tatcaaattc aaacactatg gttctatcat ctataacatg
     1501 atagaaaaaa cctgtgaaat ggaggatggt ccgcaaaaag agcaagtgat attgatgatt
     1561 gccaatttta tgaaacagtc gtatgtcaat tacaaccgcg acaatgtaaa tgatgaagtt
     1621 atttttgaac atcttcgtaa gatttccaat ggaaaacttt cgctgaaaga agaggtaaga
     1681 ttaagatata ttgctgatgt tcgacccatg aataataatg gtggtggaaa aaagaaaaag
     1741 aaaaagaaaa aatcgtcaca acattaaata atggctaaca gttttgaaat tacgggtgga
     1801 caacagctgc atggtgagat agaaccacag ggagcaaaaa acgaagcatt acagattctt
     1861 tgtgccgtat tactaaccga tgagaaagtt accattcaca acattcctga tatcagagat
     1921 gtaaacaaac tgattgactt actacgcagt ttgggtgtga ttattgaaaa actcaagcac
     1981 ggcagctata cttttcagtc agacaatata aatcttgatt ttttaaagac agatgatttt
     2041 attcgtcagg caggatcttt aagaggttct attatgatag caggtccatt gctgtcacgc
     2101 tttggcattg cctgtgtacc taaaccgggt ggtgacaaaa ttggtcgcag acgtttagat
     2161 acgcacttca taggttttca gaacctgggt gctaaatttg attttgattc tcataccaaa
     2221 atattcagcg tttcggctaa aaaactaaaa ggtgcctata tgctccttga cgaagcttca
     2281 gttaccggaa cagccaatat cattatggct gctgccttag cagaaggtac tacaaccatt
     2341 tataatgcag catgcgaacc atacatacaa caactctgca agatgcttgt cagtatggga
     2401 gcaaaaatct caggcattgg ctcaaatctg ttgagcataa caggtgtttc caaaatgaca
     2461 ggatgcacgc ataccatgtt gcccgatatg attgaggtag gaagttttat tggtttggct
     2521 gccatgactc agtctgaaat aacaataaaa aactgtagtg ttccaaatct aggcatcatt
     2581 ccctccactt ttcaacggtt gggcattaaa ctggaaatta aaggggatga catttacgta
     2641 cctgcacaaa aaaattatga aatagatact tttattgatg gttctattct gactattgca
     2701 gatgccatct ggcccggatt gacacctgat ttgctgagtg taattttagt aacagcaaca
     2761 caggcaaacg gtacagcact tattcatcaa aaaatgtttg aaagccgttt gttctttgta
     2821 gataaactga ttgatatggg ggcacagatt atattatgcg acccacaccg tgcaacagtc
     2881 atagggctta accgcaaaca ttcactcaaa ggaatagaaa tgacatctcc tgacatcaga
     2941 gcgggtgttg ccttgctcat agcagcactt tctgcaaaag gcaaaagtat tatccacaat
     3001 attgaacaga ttgacagagg ttatgaaaga atagatgaac gattgataaa acttggtgcc
     3061 catatcaaaa ggatttaaaa caatttaata ttttagcgtt taataaaaaa caaatgaagg
     3121 cttgtcactg tggtatgata gttggcagcc ataaacaaaa ttaaaaatat gacaactaca
     3181 cttaaagcaa cattcttatc gctggcaatc atcggcactg catttttttc acaggctcaa
     3241 aagccgattg aaggcattgc cataggtaac aaagcacctg aactggcatt tacgtcaccc
     3301 gaaggaaaaa ccattgcact ttcttcactg aaaggaaaaa ttgtattgat agatttttgg
     3361 gcatcatggt gcggcccttg ccgtatggaa aatcctaatg ttgttgctgc ctataataaa
     3421 tataaagata aaaaatttaa gggtgctaaa ggctttactg tttatggagt atcgcttgat
     3481 caggataaaa accgctggat gaatgccatc aaacaagata aattagattg gccaagccat
     3541 gtgagtgacc ttgccggatg gaacagcaaa gcagcacaga tttacagagt aaactcaata
     3601 cctgccaact ggcttattga tggcaacgga tacattgtgg gaagcaactt gagaggacag
     3661 gcattagatg cagccattga aaaactgctg gcacagtaaa taaaatttaa tactactaat
     3721 gaaaaacagc cggaaattcc ggctgttttt tttatgctct tatatacact tgttttactg
     3781 gaactccaaa ataagaagta taagtttttt catcacattt aattttttgt tatttgatta
     3841 atttgttctc ttagttgggc tatttcattt tttaattcgt tgagcatggc tgttacatct
     3901 tgcttttggc tttcgtttgc tatgatgagt tgtttggtgg ttatggtgcc gtttacttct
     3961 aattcacatt tgcaaaacgt aaacgatgtg gtatggcgaa ggcggctgtg tatgtagaaa
     4021 agcgcagctt acgcttaaag cgcgcgaaac gtgcaagtgt ttctgttgta atcatggtag
     4081 tttagtttaa tttacagaat tgtaaaagta a
//
LOCUS       DAOQNP010000137         4069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_9982_length_4069_cov_10.056552, whole genome shotgun sequence.
ACCESSION   DAOQNP010000137 DAOQNP010000000
VERSION     DAOQNP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9982_length_4069_cov_10.056552"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PKY09_12225"
     CDS             <1..1112
                     /locus_tag="PKY09_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13659.1"
                     /translation="ISSFGACNGMAPVVVTGGVQPYTCTWYPGNQTGLAPTNLCAGEN
                     YFTILDANGCKVSNSIVLKQPERDDWTMNGNTGSNPVNNFIGTADSNDFVFRTNNVER
                     MRIKGSGDLNIIKSLNIENYLTFKNSSFIGFEPATANSKAIMSFGAPPNTTMSISTCK
                     NPSLNTPPNYQFNGTIQLYGFAPSGGFSNIMEVGFDGINAVIDATGTSLDPDANRLLL
                     NYYCGHDVFVGNSNNGDLTANRNMLVNGKIGIGISQQDLNAIPLGYRLIVEGTIGARE
                     MRIVNPSLTWPDYVFADDYKLKSLSDIEQFVESNNRLPGFASAATVASEGQDIGALQV
                     QQQQTIEEMYLHIIALEKRISKLEQENKKLKSQKP"
     gene            1109..2704
                     /locus_tag="PKY09_12230"
     CDS             1109..2704
                     /locus_tag="PKY09_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13660.1"
                     /translation="MSRYFLVLIVCLIYNCANGQQVVQRVYTDFSATRIIKSNDGGYL
                     LAGTHDVSLWQNNAIISKIDSIGQRIWTKAFEDTIGFHNLIDIIATSDQNYLAVFLTN
                     TSNPIKLIKFNNNGDILTTTHYISNSIYSLRGVFEAENGNIIAYGFVLSTSIFSGPVD
                     FFIMKTDSNLNVIWAKQINYKYQSQFIRGILLSDGSYLFQGTAFDTTTTPTRNDLVLA
                     KTDTAGNFIWFKQSGNYQVGSGMGNGPAVVTNYGDITEKNGSIYNAFNVSWFSNFYQY
                     DVIIQKLDLNGNEIVAHQYGNDAGGTHDTRTIACTNFNEILFSTNGHLVKTDENLNFI
                     WAKRNHPNQQQISIFNSLTISDSNYIGVLNYTNLPHSNSLLVKTDTSGEISCKNVPNA
                     PLFNQQCIIGSFDKMNLITSNNVSINVVGVFHQSQNYTLINDSLQCLTSTNFEDEFSK
                     QNTIIALHNHIVTDFLRVLYLSNDYSKSQLFIYSVSGNLILKMEILPQPINNISLLEL
                     PSGLYTLIFKNKNYFQALKFVKI"
     gene            2734..>4069
                     /locus_tag="PKY09_12235"
     CDS             2734..>4069
                     /locus_tag="PKY09_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13661.1"
                     /translation="MCCLLQQIVLSQDIINPYLSYSTNQYPSIFYGGNVPLNYNFEHP
                     MVFGDISSYKSPNNLYYLGEYELCVRKHFWWDLLGFENVPFPTNVSVSYQYEMVSFPF
                     TMNCAFTNSTNQNSSDPPGCNFESTQDRFLYMPDPSSTQHVNTLTEDYWVYSVNGLTW
                     SNKLYNKTNIQKIDASGYSSGVLAGAGSPYIANTLVSAAFEATNGIGNSNGNEDRKAI
                     PHTVIKHTLNLHNGVNASPSNIVLSYFFYYDNTRGRMRFYPFVPINSQGGSQNSHDVI
                     FVPEIITNLHQDNAGNYDKPHEYDDNYNSDLILPGGVLNYFPTGELPLYPLCGNSPCS
                     LPLPGNTFATGPFVDFIYPSPFTLYSAPLRSSGSQYLAGYELNQSSQIAPLPGIRHSY
                     FIDQNTDLNIINPTEKEIYNPSETTITASNFVFPQDYTFKTIRGVYPSEAEAL"
BASE COUNT         1363 a          720 c          708 g         1278 t
ORIGIN      
        1 acataagcag ctttggtgct tgtaatggta tggcaccggt agttgtaacc ggtggtgtgc
       61 aaccttatac ttgtacgtgg tatccaggca accaaacagg tttggcaccc actaaccttt
      121 gtgcaggaga aaactatttt actatccttg atgccaatgg ctgcaaagta tctaacagca
      181 ttgtattgaa gcaacctgag cgtgacgact ggactatgaa tggcaacact ggcagcaacc
      241 cggtaaacaa ctttattggt acggcagata gtaacgactt tgtttttaga accaacaatg
      301 tggagaggat gcggataaaa ggtagtggcg atttaaacat aattaaaagt ttgaatattg
      361 agaattatct gacatttaaa aacagtagct ttattggatt cgaaccggca actgccaata
      421 gtaaggcaat aatgagtttt ggggctccac caaatacaac aatgtcaatt agcacttgca
      481 agaatccgag tttaaatacc ccacccaatt atcagtttaa cggaaccatt caactctatg
      541 gatttgctcc ttctggtgga ttttccaata ttatggaagt gggctttgat ggaatcaatg
      601 cagttattga tgccacaggc acatcgttgg atcctgatgc aaacagattg cttttaaatt
      661 actattgcgg gcatgacgta tttgtgggaa acagtaataa cggagattta accgcaaaca
      721 gaaacatgct tgtaaacggc aaaataggca ttggcatcag ccaacaagat ttaaatgcta
      781 ttccattagg ataccgatta atagtagaag gaacaatagg agcaagagaa atgcgcattg
      841 ttaacccatc attgacctgg cctgattatg tttttgctga cgattacaaa ttaaaaagcc
      901 tatctgatat agaacaattt gttgaaagca acaatcgcct gccgggattt gcatcggctg
      961 caaccgtggc atcagaaggg caagacatag gagcattgca ggtacagcaa cagcaaacta
     1021 ttgaagagat gtaccttcat ataattgcct tggagaagcg tatcagcaaa ttagaacaag
     1081 agaacaaaaa actcaaatct caaaaaccat gagccgctat tttttagttt taatagtctg
     1141 tttgatttac aactgcgcca atgggcagca ggtggtgcag cgggtctata ctgatttttc
     1201 tgctacaaga ataattaaat caaatgacgg gggatattta cttgccggaa cccatgatgt
     1261 aagtctgtgg caaaacaatg cgatcattag taaaatagat agtataggtc agcgtatttg
     1321 gacaaaggct tttgaagata ccattggttt tcataattta attgacatta tagctacttc
     1381 tgatcagaat tatcttgcag tttttctaac aaatacaagt aatcctatta aattaatcaa
     1441 gttcaataat aatggagata ttttaaccac cacgcattat atttctaatt ctatttactc
     1501 tctacgtggt gtattcgaag cagaaaatgg aaatattatt gcctatggtt ttgttctaag
     1561 tacatccatt ttttcggggc cagttgactt ttttattatg aagacagatt ctaacttgaa
     1621 tgtaatttgg gctaagcaaa ttaattacaa atatcaaagt caatttatta gaggcatatt
     1681 gttaagtgat ggcagctact tatttcaagg tactgccttt gacacaacaa caacacctac
     1741 acgtaatgat ttagttttag ccaaaacaga cactgccggt aattttatat ggtttaaaca
     1801 atcaggtaat tatcaagtgg gtagtggcat gggtaacgga ccggcagtag taacaaatta
     1861 tggtgatatt actgagaaaa atggttctat ttataatgca ttcaatgtta gttggttttc
     1921 aaatttctat caatatgatg ttatcattca aaaacttgat ttgaatggta atgaaatagt
     1981 agcccatcaa tatggaaatg atgcaggtgg aacacatgac acaagaacta ttgcatgtac
     2041 taattttaat gagattctat tttctacaaa tggacactta gtaaaaactg atgagaatct
     2101 gaattttatt tgggctaaaa ggaatcaccc aaaccaacaa caaatatcaa ttttcaactc
     2161 attaacaata tctgattcaa attatatagg agttttaaat tatactaatc ttccacattc
     2221 aaactcttta ctagtcaaga ccgatacttc aggtgaaata agctgtaaga atgttcctaa
     2281 tgcacctctt tttaaccaac aatgtatcat aggatctttt gataaaatga atctaattac
     2341 ttcaaacaat gtttcgataa atgtagtagg tgttttccat caatcacaaa attatactct
     2401 tataaatgat tcgctacaat gcttgacttc aactaatttt gaagatgagt tttcaaagca
     2461 aaacaccatc attgctttac ataatcatat tgtaacagat tttttgagag tactttacct
     2521 ttcaaatgat tattcaaaat ctcagctatt tatttattca gtgagtggca atttaatact
     2581 aaagatggaa attttacctc agcctattaa caatatttca cttttagaac tcccttcagg
     2641 tttatatact ttaattttta aaaataaaaa ttatttccaa gcattaaaat ttgtaaaaat
     2701 atgaaacgat gttttcaact gtcaattatc gcgatgtgtt gtttattaca acaaattgta
     2761 ttatcacaag atattattaa tccttattta tcttattcaa caaatcaata tccttctata
     2821 ttttatggag gtaatgtgcc actaaattac aacttcgagc atccaatggt ttttggtgat
     2881 atttcatcat acaaatcgcc taataattta tattacttag gagaatatga gctctgtgtc
     2941 agaaaacatt tctggtggga tttattagga tttgaaaatg tgcctttccc tacaaacgtt
     3001 tctgtttcgt accaatatga aatggtgtct ttccccttta caatgaattg tgcttttaca
     3061 aacagcacta atcagaatag ttctgatcct ccaggatgta attttgagag tacacaagat
     3121 agatttttat atatgcccga cccaagcagc acgcaacatg taaatacttt aacagaagat
     3181 tattgggttt atagtgtcaa tggtttaaca tggtcaaaca agttgtataa taaaactaat
     3241 attcaaaaaa ttgatgcaag tggttattct tccggggtat tagcaggagc aggtagccca
     3301 tatattgcca acacacttgt atcagcagct tttgaagcta caaacggtat tggaaacagt
     3361 aatggcaatg aagataggaa agcaatacct cacaccgtaa ttaaacatac attaaattta
     3421 cataacggag taaatgcttc accttcaaat attgtattat cctatttttt ttattatgac
     3481 aatactagag gaagaatgcg cttttatcca tttgtgccaa taaatagtca aggtggttct
     3541 caaaattctc atgatgtgat ttttgttcct gaaattatta ctaaccttca ccaagataat
     3601 gcaggtaact atgataagcc acatgaatat gatgacaatt ataatagcga tttaatttta
     3661 ccgggaggtg ttttaaatta cttcccaact ggtgaacttc ctctgtatcc cttatgtggt
     3721 aattccccct gcagtttgcc attaccagga aacacatttg ccaccggtcc attcgttgat
     3781 tttatttacc catcaccatt taccttatac tctgcaccat tacgaagttc cggctctcaa
     3841 tatctggcag gatatgaatt gaatcaaagt agtcagatag caccactacc cggcatccgc
     3901 cacagttact ttatagatca aaacaccgat ttaaacatca tcaacccaac agaaaaagag
     3961 atatataacc ccagcgagac taccatcacg gcaagcaact ttgtttttcc gcaggactat
     4021 acttttaaaa ccatacgagg ggtgtatcca agcgaggcag aagcccttg
//
LOCUS       DAOQNP010000138         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_9999_length_4066_cov_5.494889, whole genome shotgun sequence.
ACCESSION   DAOQNP010000138 DAOQNP010000000
VERSION     DAOQNP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9999_length_4066_cov_5.494889"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2296
                     /locus_tag="PKY09_12240"
     CDS             <1..2296
                     /locus_tag="PKY09_12240"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13662.1"
                     /translation="AHLTVIGTTFTKVNGFKPDYVQQYPHGTVPYCGILLQKMFCTIG
                     DDNQNENYFSKMIYGIFSNNSDLTLTNSRFQYIYNHNSWMSKAKGTAVVSWGNSQNKL
                     GSLKMRPVVSGNATIEQCDRGLYTTYSAVDVNRLIIKQVLTGMFTANCYGRDAQISYN
                     NIEAKSYGVYLQSNAGSIKCNVENNVINMKGSSNGIGITLNENTSANANYIITGNNIT
                     MDNSGDGIKVNNVTRPVISYNVIYQNGNTPPSNGTRGIALLGCDMATVSCNYVQSTYN
                     NLLVGSYGLYSSLSTNASITCNIVEGQNEGVFFGGYCGGTDFKGNNMKDNYVGLYLNN
                     VAIIDKQPANGVPPYQGNEWTGTFNSSFGAANMNATSPFDLLFNLFTTNQTLSIHNPL
                     IPNGSQLQFNGVDNTGWFAPQPNGITFNCTVSGVCNAMRSTSDGSVELTQLIAKDSAL
                     TNDYVEESKLMAQQYLYDVLTTDTLLLQSDTVYHNFKGQKEIESVGLLYDVKEHFTTI
                     KNTVSSLATITALNDSLINAIKQTIKNCDSLFAATNNDSLLATKQALIDSLNNVAIQN
                     NLIWQNKAAQDSASLTAATNINMIIATQQTPDANEKLLNEILIDIETNGDTILSSYNV
                     ALSTIANQCPYEGGAAVYKAQALLQLLNDTIVFETDNTCWQNGYYRKAFGENRSLVDE
                     TGISIVPNPANEETTVILKRVEEGFCAIELNDVLGQLVFQATFNCADKRHKISTKHLK
                     QGVYTLSVSINSCIMKKSKLIIVR"
     gene            2293..3786
                     /locus_tag="PKY09_12245"
     CDS             2293..3786
                     /locus_tag="PKY09_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13663.1"
                     /translation="MKKLTIIMLIALSFKTYGQGYNHQWLLGYFYFIQDPKGRLLFDS
                     SSYTLVNETRKMVFMGTEANISDSNGNLLMSSNGVWIADATGDTMQNGSGLNPWGVTS
                     SWPYGLPMMNNNLLLPLPNDTFEYILFHHTATQLNSIHKCFELCYSLIDINQNSGLGQ
                     VISKNNLIISDTLMGGIGSCKHANGKDWWIFMMKDSSDIIYKIALTDSSQMSITSQKM
                     NFSPLAVSNVAQLTFSPSGNKFVATTYDNPINRNSYVVISDFDRCTGMFSNTQTVQVT
                     SGAYIWGTAFSPSGKYIYACSSQYVFQIDAATHTVDTIATYDGFISPVGATCCPTTFW
                     NMYLAANGKIYITSGSGVQHLHEITYPDSAGTACKLQQHIINLGFGNLRAVPNHPNYY
                     LGCDTTFGCTPCYTGINEIKQHDFKFSISPNPSNGSFKIIYLLPQNKSGTLQIFDITG
                     KEIYKQNLPPWSTMQYISLPKLANGVYNCTITSNNERVHKKLVVFKE"
BASE COUNT         1412 a          716 c          752 g         1186 t
ORIGIN      
        1 tgcacacctc accgtaatag gtactacatt cacgaaggta aatggcttta agcccgatta
       61 tgtgcagcaa tatccacatg gcacggtgcc ttactgcggc atactgctgc aaaagatgtt
      121 ttgcaccatt ggtgacgaca atcagaatga aaactacttt agcaaaatga tatatggcat
      181 ttttagtaac aacagcgacc tcacacttac caacagccgt tttcagtata tttacaatca
      241 caacagttgg atgtctaagg caaagggaac agcggtggtg agttggggca acagccaaaa
      301 caagctgggc agtttaaaaa tgcgtcctgt ggtgagcggc aatgccacca tagagcaatg
      361 cgaccgtggg ctatatacca cctacagtgc agtagatgta aacaggctta tcataaagca
      421 ggtgctaacg ggaatgttta ctgcaaattg ctatggcaga gatgcacaaa taagctataa
      481 caacattgag gctaaaagct atggagtgta tttgcaatct aatgccggat caataaaatg
      541 taatgttgag aataacgtta ttaatatgaa aggaagcagt aatggaattg gcattacctt
      601 aaatgaaaac acttctgcca atgccaacta cattattacc ggcaataata ttacgatgga
      661 caattccggt gatggtataa aagtaaacaa tgttacacgg ccagttatct cttacaatgt
      721 tatataccaa aacggaaata caccaccttc aaatggtact agaggcatag ccttattggg
      781 ttgcgatatg gcaactgttt catgtaatta tgttcaatct acttataata atcttttagt
      841 tggtagctat ggtttatact caagtttaag caccaatgcc tctataacgt gcaatatagt
      901 agaaggacaa aatgaaggtg tgttttttgg tggttactgc ggaggtactg attttaaagg
      961 caataatatg aaggataatt atgtgggact gtacttaaac aatgttgcta taattgacaa
     1021 acaaccggct aacggagttc caccctatca gggtaatgaa tggacgggta cgttcaacag
     1081 tagttttgga gcggctaata tgaatgcgac ttcaccattc gacttactct ttaatctttt
     1141 cacaactaac caaactttaa gtattcataa tccattaata ccaaatggct ctcaactgca
     1201 atttaatggt gtagataata caggttggtt tgcacctcag ccaaacggaa tcacttttaa
     1261 ttgtaccgtt tcaggtgttt gtaatgccat gcgcagtaca tctgacggtt cggttgaatt
     1321 gacacaactc atagcaaaag acagtgccct caccaatgat tatgttgaag aatcgaagtt
     1381 aatggctcaa caatatctat atgatgtctt aactaccgat actttattat tacaaagtga
     1441 tacggtatat cataatttta agggccagaa ggaaatagaa tccgtaggtt tactttatga
     1501 tgtaaaagag catttcacta caattaaaaa taccgtaagc agtcttgcaa ctattacagc
     1561 attaaatgac agccttataa atgcaataaa gcaaacaata aaaaattgtg acagtttatt
     1621 tgcagcaact aacaacgata gtttattggc aacaaagcag gcgttgatag attcattaaa
     1681 taatgttgct attcagaata acctaatatg gcaaaataaa gcagctcaag acagtgcaag
     1741 ccttaccgct gcaacaaata ttaatatgat aatagcgaca caacaaacac ctgacgccaa
     1801 tgaaaagttg ctgaacgaaa tattaataga cattgaaacc aatggagaca ctatattgtc
     1861 gtcatataat gtggcattat caacaattgc caatcaatgc ccctatgaag gtggcgcagc
     1921 agtatataaa gcacaggcat tactgcaatt gttaaacgat acaatagtgt ttgaaacaga
     1981 caacacctgt tggcaaaacg gttattacag aaaggccttt ggggagaata ggagccttgt
     2041 agatgaaacg ggtattagca tagttccaaa tccggcaaac gaagaaacaa cggtaatatt
     2101 gaaaagagtt gaagaaggtt tttgtgccat tgagttaaat gatgtattag gacaattggt
     2161 gtttcaggct acattcaatt gcgcagataa acgacataaa ataagcacaa aacatctcaa
     2221 acaaggcgtt tatactttat ccgtgagtat caatagctgc ataatgaaaa aaagtaaatt
     2281 aataattgtg cgatgaaaaa gctgacgatt atcatgctta tagccttgtc ttttaaaacc
     2341 tatggccagg gatataatca tcagtggctt ttaggctatt tttactttat acaagaccca
     2401 aaaggccgcc tgttatttga ctcaagtagt tataccttag tcaatgaaac aagaaaaatg
     2461 gtatttatgg gaactgaagc aaacataagt gactccaacg gcaatttgtt gatgagcagc
     2521 aatggtgtgt ggatagctga tgctactggc gatactatgc aaaatggaag tgggttgaat
     2581 ccttggggag ttacaagtag ctggccttac ggtttgccaa tgatgaataa caatttactt
     2641 ctaccactac caaatgacac atttgaatac attctgtttc accacacagc aactcaatta
     2701 aattctattc ataaatgttt tgagctgtgt tattcattga ttgacattaa tcaaaattca
     2761 ggtttaggac aagtaatttc taaaaataat cttattattt ccgataccct tatgggaggt
     2821 attggttcat gtaaacatgc taatggaaaa gattggtgga tttttatgat gaaagatagt
     2881 agtgatataa tttacaaaat agccttgaca gacagcagtc aaatgagtat cacatcacaa
     2941 aaaatgaatt tttctcctct tgcagttagt aatgtagccc aattaacatt ttcaccaagt
     3001 ggcaataaat ttgttgcaac cacctatgac aatccaataa acaggaacag ctatgtagtt
     3061 attagtgact ttgaccgctg tacaggcatg ttttccaata cacaaacagt acaggtaaca
     3121 agtggtgctt atatatgggg cacagcattt tctccatcag ggaaatatat ttatgcttgt
     3181 agtagtcagt atgtttttca aatagatgct gcaacccata cggttgacac tattgctaca
     3241 tatgacggtt ttatttcacc cgttggagca acatgttgtc ctactacatt ctggaacatg
     3301 taccttgctg ccaatggtaa aatatatatt acaagtggta gtggtgttca acacctacat
     3361 gaaattactt accctgatag tgccggaact gcctgcaaat tacaacaaca tataataaat
     3421 ttaggatttg gtaatcttcg tgctgtcccc aaccacccca actattacct tggctgcgat
     3481 actacgtttg gctgtacgcc atgttataca ggcataaatg aaattaagca acacgatttt
     3541 aaattttcaa tatcccccaa cccatcaaac ggtagcttca aaataattta tctcttaccc
     3601 caaaacaaaa gtggcacact tcaaatattt gacataacag gcaaagaaat ttacaaacaa
     3661 aacttaccac cttggagtac aatgcagtat atatccttac caaaacttgc aaacggtgtt
     3721 tataattgca caatcacaag caataatgaa agggtgcata aaaagttggt ggtttttaaa
     3781 gagtgatgta aaaactaagt tccaaaatca aaccgataat tggtcatgtt tcaaaatatc
     3841 accttatatt ataaatgatt gtctatcagg ttacaagaat aatttaaact ttaaaaatct
     3901 aatacaataa tttatgtaaa aaatcatttg aaaatgattt aatgagtttt ttgaaattgg
     3961 aattgtttga ttttcacctt tatgtttcaa aatatcacct atgctatggc atgtatttat
     4021 tgtaatggtt cttgtattaa aaaaggtttt cagaaaaacg gtaatc
//
LOCUS       DAOQNP010000139         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_10058_length_4053_cov_8.404452, whole genome shotgun sequence.
ACCESSION   DAOQNP010000139 DAOQNP010000000
VERSION     DAOQNP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10058_length_4053_cov_8.404452"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKY09_12250"
     CDS             <1..200
                     /locus_tag="PKY09_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13664.1"
                     /translation="YLLPQNSKGTFEVFDITGKKVFNYNLPQWSTMQYISLPKIASGV
                     YQCIITSGNERVHKKLVVFKE"
     gene            746..1456
                     /locus_tag="PKY09_12255"
     CDS             746..1456
                     /locus_tag="PKY09_12255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNB13665.1"
                     /translation="MTTQSGVKIEIKEKGSGIKPSQGDRITVHYTGKLQDGTKFDSSV
                     DRGEPFVFNVGTGQVIRGWDEAFGMLQQGDKAVLTIPPDAGYGSRAVGTIPANSTLIF
                     EVELLRVSPKIVPMPYNVAGKDTVKLPSGLQYIMVKEGTGKSAASGATVAVHYSGYLE
                     DGSMFDSSVERGEPISFRLGEGRVIQGWEQGIALLKTGSQCRLIIPYQLGYGEQGYPP
                     VIPAKSTLIFDVELIEVN"
     gene            1478..2749
                     /locus_tag="PKY09_12260"
     CDS             1478..2749
                     /locus_tag="PKY09_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13666.1"
                     /translation="MKKVLLITYYFDPGNAVATPRVMSWANEFHQYGIELTVVTRHFK
                     GDEWHWLDFIKETTEPVKVIKNERFSVHYLPYQSGKPALANRNKLYSKFYHLKTFLEG
                     HFNTEINAYKAFGNYCRELLRNNPHDLILVSAPPHNLVRLASELGKEFDLPYVVDFRD
                     IWNNLLTGKVEMNMRGRISNFIEEKFIGKWLNGASMVTTVSEALIEQVKRIYNGKCIE
                     LTNGYEMKLFSQVSNTNPPSDRFVFASIGTIYPIQNISIMIDGLKIFYDKLRDKSKFE
                     VKFIGLGAIESVAHQVTSELESFKPLVTQKLSRKEVLRHYNDSHVLFIPAWSNHKGIY
                     STKIFEYIGSGRKILVAPGDNDVIDNLVRKSGRGYIANSAEEFASSLNEIYNLWLNGN
                     YKVEQPAEWEQFYSREKQAALLANEILEIAR"
     gene            2746..3864
                     /locus_tag="PKY09_12265"
     CDS             2746..3864
                     /locus_tag="PKY09_12265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNB13667.1"
                     /translation="MKEKICFILSFHDRAVQYEWIIERAIQHNLELHFIFLNPEQWHI
                     EEFARMKKIPCLFIKYHSKKDIPRAIALIFQYLVKEKITVVHTHLFDANLAGLTAAWL
                     AKVKKRIYTRHHSDFHHRFYPKSVRYDRYCNRLATDIVAISKNVYDILVEKEKVKPNK
                     VHLVHHGFEMDLFSKVPVSQIEELKKKYKTKDRFPVVGMISRYEESKGIQHVIPAFKQ
                     LLKKYPNALLILANSHGSYSKQIKKALEQIPTESYCEIRFEYNLFALYQLFDVFVHVP
                     EYKEYEAFGQVYVEALAAGIPSVFTISGIASEFVKNNENALVCDYKNPDEIYRAIIKL
                     LSDSNLRNKLVENGLKEVHSKFSMDKYFSHLKKLYGYD"
     gene            3857..>4053
                     /locus_tag="PKY09_12270"
     CDS             3857..>4053
                     /locus_tag="PKY09_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13668.1"
                     /translation="MTDLTANEHTFHVFMFYESDTDSFMKQIAFVQRQYEKNVVLHVA
                     YLKKDFNVLESIIIELNQGSKT"
BASE COUNT         1376 a          599 c          834 g         1244 t
ORIGIN      
        1 tgtacttgtt accgcagaat agtaagggaa cttttgaagt gtttgatata acaggcaaaa
       61 aagtttttaa ctacaacctg ccacaatgga gtaccatgca atatatatca ctgccaaaaa
      121 ttgcaagcgg agtttatcaa tgtataataa ctagtggaaa tgaaagggtg cataagaaat
      181 tggttgtctt taaggagtaa taaaaaacaa acactcaaac ctaatatacc atgtttacaa
      241 agtttccttc atggctcaaa taccgacctt ctgaaaaaac tcgttaaaca aaagccacct
      301 gtatgagcaa aaagaaaaca aactgtctga agttgccggc atatccacca gtggcggatt
      361 gccgcaacaa gtttttattt tcgtgcgtag ctggcttttg tagtggtttt tcagacagtc
      421 ttgatttttg ggttcttttg tgtcaagaca aaagtatgta ggaagattca ttagactctc
      481 aggctttgga gtatcattca gtaaatctca ttgccaaaaa tcaattacga tgtttaccaa
      541 ttggtgattt caagtggtaa tgaaagctta cataaaagtt ggttgtcttt aaggagtgat
      601 aaaagttgaa aagtaatttt tattaataaa aaaatatagt atcacaccga gcatgaaaaa
      661 actaatgtta tgtttgcttg ctataatgac tgcctgcaaa tcaggtataa agtaattgga
      721 aacacatcat attcaaacaa aatttatgac aacacaatca ggagttaaaa ttgaaattaa
      781 agaaaaagga agcggaatta aaccatcaca gggtgataga attaccgttc attatacagg
      841 taaattgcag gatggcacta agttcgatag ttctgttgac agaggggaac cgtttgtgtt
      901 caatgttgga acaggacaag tgataagagg atgggatgaa gctttcggta tgcttcagca
      961 aggtgacaag gcagtattaa caataccacc tgacgcaggc tatggcagcc gtgcagttgg
     1021 aactattccg gcaaactcta ccctgatttt cgaggtggaa ttacttcgcg tgtcgcccaa
     1081 aattgttcca atgccatata atgttgcagg taaggataca gttaagttac catccggact
     1141 tcaatatata atggttaaag aaggaacagg caaatctgct gcttcgggcg caacagtagc
     1201 tgtgcattac agcggatatc ttgaagatgg aagcatgttt gattcatctg ttgaaagagg
     1261 tgagccaatt agttttcgct tgggtgaggg aagagttatt caaggatggg agcaaggtat
     1321 tgctttatta aaaaccggct cacaatgcag attgattatt ccttatcaac ttggctatgg
     1381 ggagcagggt tatccacctg taattcctgc aaagtcaaca ctcatttttg atgttgaatt
     1441 aattgaggtc aattgatggc aattgtgaaa tgctgaagtg aagaaagttc tgcttataac
     1501 ctactatttt gatcctggaa atgctgtcgc cacacctcga gtaatgagtt gggcaaatga
     1561 attccatcag tatggcattg aacttacagt tgttaccaga cattttaaag gtgatgaatg
     1621 gcactggctc gattttatta aggagacaac agagcccgtt aaagtaatta aaaatgaacg
     1681 tttttctgta cactaccttc catatcagtc aggcaagcca gcgcttgcaa ataggaataa
     1741 actttacagt aaattttatc atttaaaaac atttcttgaa ggacatttta atacagaaat
     1801 caatgcttat aaagcatttg gtaactactg cagagagttg ttgaggaaca atccgcatga
     1861 tttgatttta gtttctgctc caccacacaa tcttgtcagg ttagcaagtg aattaggcaa
     1921 ggaatttgat ttaccatatg tggttgattt tagagatatc tggaacaacc ttctgacagg
     1981 aaaagttgaa atgaatatgc gtggtcgtat ttcaaatttt attgaagaaa aatttattgg
     2041 taagtggttg aatggggcca gtatggtcac cacagtgagt gaggcactaa ttgaacaagt
     2101 taagagaatc tataatggta aatgtataga acttaccaat ggatatgaaa tgaagttgtt
     2161 tagtcaggtg agtaatacaa atcctccatc ggatcgtttt gtttttgcaa gcatcggaac
     2221 tatctatcct attcaaaaca ttagtataat gattgatggt ttgaaaatat tttatgataa
     2281 actaagagat aaaagtaagt ttgaagtaaa atttatcgga ttaggcgcaa ttgaaagtgt
     2341 tgcacatcaa gtgacttcag aacttgaatc ttttaagccc ttggttacac aaaagttgag
     2401 caggaaagaa gtgctaaggc attataatga tagtcatgta ctttttattc cggcatggtc
     2461 aaatcataaa ggtatttact caacaaaaat ttttgaatat ataggctcag gacggaaaat
     2521 cctggtagcc ccaggagaca atgacgttat tgataattta gttcgtaaat ccggcagggg
     2581 ttatatagcc aatagtgcag aggagtttgc ctcttcatta aatgaaatat acaacttgtg
     2641 gctgaatgga aattataaag tagagcaacc ggcagagtgg gagcagtttt attccaggga
     2701 gaagcaagca gcacttcttg caaatgaaat tttagaaatt gccagatgaa agaaaagatt
     2761 tgttttattt tatcgtttca tgatcgtgct gtacaatacg aatggattat tgaacgtgca
     2821 attcaacata acttggaatt gcattttatt tttttgaatc cggaacagtg gcatattgaa
     2881 gagtttgcaa ggatgaaaaa gattccttgt ttatttataa aatatcattc taaaaaagat
     2941 atccctcgtg ctatagcatt aatttttcaa tatctggtaa aagaaaaaat taccgtagtg
     3001 catacgcatc tctttgatgc taatcttgca ggactcacag cagcatggtt agctaaggtc
     3061 aaaaagagaa tttatacccg acaccattcg gattttcatc atagatttta tccgaaatcg
     3121 gttcgatatg atcgttattg caacaggttg gctactgata tagtggcaat cagtaaaaac
     3181 gtatatgata tattagtcga aaaggaaaaa gtaaaaccca acaaggttca tttagtgcat
     3241 catgggtttg agatggattt attttcaaaa gtgccggtat ctcaaataga ggaattaaag
     3301 aagaagtata aaacaaaaga tagatttcct gttgttggca tgataagtcg ttatgaggag
     3361 agtaaaggaa ttcagcatgt catacctgcc tttaagcaat tattgaaaaa atatcctaat
     3421 gcacttctga tattggcaaa cagtcatggt tcatattcaa aacagattaa aaaagcactt
     3481 gagcaaattc cgactgaaag ctattgtgag attagatttg agtataattt atttgcattg
     3541 tatcagttat ttgatgtatt tgtgcatgtg ccggagtaca aagagtatga ggcatttggg
     3601 caagtttatg ttgaagcact tgcagcaggt attccttctg tttttaccat ttcaggaatt
     3661 gcctctgagt ttgtaaaaaa caatgaaaat gcacttgttt gtgactacaa aaatcctgat
     3721 gaaatttatc gtgctattat aaaattactc tcagatagta atctgagaaa caagttggtt
     3781 gaaaatggat tgaaagaagt gcattcaaaa ttcagtatgg ataagtattt tagtcatctt
     3841 aaaaaacttt atgggtatga ctgatttgac agcaaatgaa catacttttc atgtttttat
     3901 gttttatgaa agtgatacag atagtttcat gaaacagatt gcttttgttc aaaggcaata
     3961 tgaaaaaaat gttgtattgc atgttgcata tctgaaaaag gattttaatg tacttgagag
     4021 tataattatt gagcttaacc aaggtagtaa aac
//
LOCUS       DAOQNP010000140         3893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_10793_length_3893_cov_6.813966, whole genome shotgun sequence.
ACCESSION   DAOQNP010000140 DAOQNP010000000
VERSION     DAOQNP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10793_length_3893_cov_6.813966"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..2339
                     /locus_tag="PKY09_12275"
     CDS             213..2339
                     /locus_tag="PKY09_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13669.1"
                     /translation="MNFSIEKCYHLTTQQYFISSDHSYGPTNDLENFDQTTCDVQIQN
                     DFNHVATMGFNSIRIAGFYPTYVSGQGLVFRFTNHDLSGTTIVPLDPLNVSDPGMLTI
                     LPMYNHILSLANSAVNTTTSQPTPLKVIFIITGSKSPYDATEVTAWNNFLAALSTYMA
                     TASNANALLAYDLMNEPCYSFSVEKTKEEACSMFAGWYDIIKSNDPLHLVTIGSCGRS
                     DVFSADPAILKLDFLSLHYYPDWKEAIEDRTLTSIQDRMKTRSVNELYWINENSPMPW
                     IVGETGFTANLVDANNLDWKTRNLHGTLADQSNYAAYSLDAVCNCGSSGYSWWAYQDV
                     HYCSWPPNPPPLFCSGTNDYAGNFFGLLYWDTYPSTASDKPAATHFPTYQPIVNGSCP
                     VDRTPLYDPNKLYYNSNLYPPNPTHEVTRTLIDQNGNPIKNAVVACHFHIGIDQFTND
                     ELYDLFYTNTDDNGSFTALPMDYTFPYNTDALWLTKVVISAPGSERLKWDWCPGCVVL
                     PPSPLTLKKIDFNYDGVLQNQTLTASTFPTNYKGWNTLTLTNVDIQPGFAGNFTARNE
                     VKVNSEFHAANGTEVHLYCQPQVFSNCNDFQTFLMQRQASSLSANSANDEKQIEIVFD
                     KITDAFISIFPNPNNGIFTVQLHDNNELNKIKTVSITNSIGGLVYSSMVNNSMVELNL
                     SHLSKGIYYAKIITGQNAEIKKIIIN"
     gene            2351..>3893
                     /locus_tag="PKY09_12280"
     CDS             2351..>3893
                     /locus_tag="PKY09_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13670.1"
                     /translation="MENIKYTISLILFFAINQQVCSQNWQWANKAGGTGYDTGSSVAE
                     DNSGNVYATFNIDGPIAYFQTDTFTVNGWDDFFLVKYDNNGNELWVKQFGGSYNSSTI
                     IKSEGAGGLKYDANSNSIYISGSFIGTCNMGSVTLTTFGNDKNWFIAKFDLNGNCLWA
                     KNIGNPYSDDIYGLAFNLTPIGDIFFTKTFNGVGYIDTVMVSGGGFLGKIDNNGTLIW
                     VKNIINAVPSSLSGGSGSGALPFSLSYFNNEIFILSYKGADTCIVDSTIQFISAANYP
                     LILSKFSIQGNVISAIQAGGPFPDGSRLSMDNSGNCYFASRFSGGIAIFGNDTIHATG
                     TTDFFFAKYDQNLNYQWVQQSHTSNTARAIGCSADANGNVYITGSFSGNATFGTFNIT
                     SNTSEDMFIARYDNIGNCIGVRHEGQGFGYDILTTQDGSCIVTGNYNGNITFSGIPTL
                     TGYGGNDIFIAKSDAITGIGEGGRLANNQLIIYANPNQGKCNITIPDDFLNEANLVLS
                     IYDNTG"
BASE COUNT         1278 a          708 c          692 g         1215 t
ORIGIN      
        1 tgaagagcaa actttgtaca ttattagttt gcaaaaacaa gtggatgaat tgaagacaaa
       61 ggtgaaaaat ttagaaagga ggaaataata tgaaaaccaa aattttatta tccttcttat
      121 cggttatatt ttactcatgt ctgttagctc aaaatgtatc aattgaggga aagcagttta
      181 aattaaatgg gagtaacttc tatccaatga ttatgaattt ttctattgaa aagtgctatc
      241 atttaacaac gcagcaatat tttatttctt ctgaccactc ttatggcccc acaaatgatt
      301 tagaaaattt tgaccaaaca acctgcgatg tacaaattca aaacgacttc aatcatgttg
      361 ctacaatggg ttttaattca atcagaattg ccggatttta tccaacttat gtgtcagggc
      421 aagggttggt atttcgtttt acaaatcacg acctttccgg cactacaatc gttccgcttg
      481 accctctaaa tgtttctgac cccggaatgc taaccattct tcctatgtat aatcatatac
      541 tatcgttagc taacagcgca gttaatacta ccacttcaca accaactcct ttaaaagtga
      601 tatttattat cacaggttct aagtcgcctt atgatgcaac cgaagttaca gcatggaata
      661 attttcttgc tgcattatct acttacatgg caacagcatc caatgcaaat gctcttttag
      721 cttacgattt aatgaatgaa ccctgttata gctttagtgt tgaaaaaaca aaggaagaag
      781 catgttcaat gttcgctggt tggtacgata ttattaaaag caacgacccc ttacatttgg
      841 taacaattgg ctcatgcgga agaagcgatg ttttttctgc cgaccctgct attcttaaac
      901 ttgatttttt gtcgctgcat tattaccctg attggaaaga agctatcgaa gatcggactt
      961 taacgtcaat tcaggacaga atgaaaacaa gaagtgttaa tgaactgtat tggattaatg
     1021 aaaattctcc catgccttgg attgtaggag aaacaggatt tacggcaaat ttagttgatg
     1081 cgaataatct cgattggaaa acaagaaatt tacatggcac cttggctgac caatcaaact
     1141 atgcagctta ttcattagat gccgtttgca attgcggtag tagtggttac agttggtggg
     1201 catatcagga tgtacattat tgcagttggc ccccaaatcc accaccttta ttttgttcag
     1261 gtactaatga ttacgctggt aatttttttg gccttcttta ttgggacaca tacccttcca
     1321 ctgcttctga taaaccagcc gcaacacatt tcccaactta tcaaccaata gtaaatggaa
     1381 gttgccctgt tgaccgcacc ccactttacg acccaaacaa actttactac aatagcaatt
     1441 tatatcctcc taatccgact catgaagtta cacgaaccct tattgaccaa aatggcaatc
     1501 caattaaaaa tgctgtggtt gcttgccatt ttcatattgg gattgatcaa tttactaatg
     1561 atgagctata cgatttattt tatacaaata ctgacgacaa tggctctttt acagcattac
     1621 caatggatta tacatttcct tataatactg atgcactatg gctgaccaag gtagtaattt
     1681 cagcccctgg ttctgagcgt ttaaaatggg attggtgtcc gggatgcgtt gtgctaccac
     1741 catcacccct taccttaaaa aaaatagatt ttaattatga tggcgttttg caaaaccaaa
     1801 cacttaccgc aagcaccttt ccaaccaact ataaaggatg gaacacgcta actttaacaa
     1861 atgttgatat tcaacccgga tttgcaggta attttactgc ccgtaatgaa gttaaagtca
     1921 atagcgaatt tcatgctgcc aatggtacag aagttcattt gtattgccag cctcaggtgt
     1981 tttcaaattg taatgatttt caaacatttt taatgcaaag gcaggcaagc agtttgtcag
     2041 ctaactcagc caatgatgag aaacaaatag aaatagtatt tgataaaatt acggatgcct
     2101 ttatctctat tttcccaaac cctaataatg gtatctttac tgtgcaatta catgacaata
     2161 atgagttaaa caaaattaaa accgtttcaa taaccaatag cataggcggc ttggtttatt
     2221 caagtatggt taacaacagc atggttgaat taaatctttc acatctctca aaaggaattt
     2281 attatgctaa aattataacc gggcaaaatg cagaaattaa aaaaatcata ataaactaaa
     2341 acaatataaa atggaaaata taaaatatac catatcattg attttattct ttgcaataaa
     2401 tcagcaagtc tgttcacaaa attggcaatg ggctaataaa gccggtggga caggatatga
     2461 taccggttct tcagtagctg aagataattc aggtaatgtg tatgctacat ttaacataga
     2521 tggaccaata gcatattttc aaacagatac ctttaccgta aatggttggg atgacttttt
     2581 tcttgttaaa tacgataata atggcaacga actttgggtg aaacaattcg gaggctcata
     2641 taatagctca accattataa aatctgaagg tgcaggtggt ttaaaatatg atgcaaattc
     2701 aaatagcata tacatttcag gtagctttat aggcacttgt aacatgggtt cagtaacgct
     2761 cactaccttt ggaaacgata aaaactggtt tattgccaag tttgatttga atggcaactg
     2821 cttatgggcg aaaaatattg gaaacccata tagtgatgat atttatggtt tagctttcaa
     2881 tttaacacct attggagata ttttttttac gaaaactttt aacggtgtag gatatattga
     2941 tacagtaatg gtatccggtg gcggtttttt aggtaagata gataacaacg gaactctcat
     3001 atgggttaaa aatattatta atgctgttcc atcaagttta tcaggcgggt caggatcagg
     3061 tgccttaccc ttttctcttt catattttaa taatgaaata tttattttaa gttataaggg
     3121 agccgatacc tgtatagtag atagcacaat acaattcatt tctgctgcta attatccatt
     3181 gatactttct aagttttcaa ttcagggaaa tgtaatttcc gcaattcagg caggcggacc
     3241 tttccctgat ggctcaagat tatcaatgga caactccggc aactgctatt ttgcaagtcg
     3301 tttctccggt ggcatagcaa tatttggtaa cgataccata cacgcaacag gcacaacaga
     3361 tttctttttt gcaaaatatg accaaaacct aaattaccaa tgggttcaac aaagccatac
     3421 gagcaatact gcaagagcta ttggatgctc ggcagatgca aatggtaatg tttacattac
     3481 aggaagtttt tctggcaatg caacatttgg tacatttaat attacatcta acacctcaga
     3541 agatatgttt attgcaaggt atgataatat cggaaattgc attggagtga gacatgaagg
     3601 gcaaggtttt ggatatgata ttttgacaac ccaagacggc tcatgcattg taacaggcaa
     3661 ttataatggc aatataacct tcagtggaat tcccacttta acaggttacg gaggcaacga
     3721 catctttatt gctaaatcag atgcaataac tggaataggt gaaggaggca gacttgcaaa
     3781 taaccagtta attatttacg caaatcccaa tcagggcaaa tgcaacataa ctattcctga
     3841 tgatttttta aatgaagcaa accttgtttt aagcatttat gacaacaccg gca
//
LOCUS       DAOQNP010000141         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_10872_length_3878_cov_9.065394, whole genome shotgun sequence.
ACCESSION   DAOQNP010000141 DAOQNP010000000
VERSION     DAOQNP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10872_length_3878_cov_9.065394"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKY09_12285"
     CDS             <1..435
                     /locus_tag="PKY09_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HNB13671.1"
                     /translation="MKVSLLAVMLIMIPLVLLLIAIVSAAIFSQEDIALSLSYGFLIF
                     FGWITAIILGMTFKTLPFIVWNKVYHKRSGIGKTPNPKDLFSNTVFKLMSLAYIVGLL
                     LFVIGSLMIHLLLIQCGAVLILTAASLYVFNVLKVVTHKPQQ"
     gene            435..758
                     /locus_tag="PKY09_12290"
     CDS             435..758
                     /locus_tag="PKY09_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNB13672.1"
                     /translation="MNVKTNNNLKSTVALAALEHVDDPEIGLNVVDLGLIYEVNFDEN
                     EKKITCIMTLTTEFCPMGESISENARNAVQQVFPEYLIDLNLTFDPSWNPEMISEEGR
                     KFLGR"
     gene            761..1192
                     /locus_tag="PKY09_12295"
     CDS             761..1192
                     /locus_tag="PKY09_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNB13673.1"
                     /translation="MLSLSCKAGIKAVIFLGSKFESGDKTGIKEISKHINENEHTVGK
                     ILQKLVKNNIIKSVKGPNGGFYITPKQKNQPIINIVEAIDGKDVFKQCGLGLTKCSES
                     RPCPFHNDFKPVRELFKNMCIEKKVSDLYQNVNNGLSYLVG"
     gene            complement(1332..1544)
                     /locus_tag="PKY09_12300"
     CDS             complement(1332..1544)
                     /locus_tag="PKY09_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNB13674.1"
                     /translation="MPIVVNLDVMMAKRKVSLNELSERVDLTLSNLSILKTGKAKAVR
                     FSTLEAICKALDCQPGDILEFVNAEK"
     gene            complement(1548..2081)
                     /locus_tag="PKY09_12305"
     CDS             complement(1548..2081)
                     /locus_tag="PKY09_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HNB13675.1"
                     /translation="MANTRFIFQVLHVIAWIFFVGLCIEAGGIIVNFVFSIYNPEFVS
                     KLYQKLDLSELYNRSKWIFFSMYSFIIVIALLKVLLFYTVIMLLLKLDLSKPFSSFVS
                     SQISQISYYTLSIGIMCLIAAQTTKNLDHHGYDVERLYQFWNDGKAFILMSAVVYVIS
                     TIFKKGVELQKENDLTV"
     gene            complement(2306..3298)
                     /locus_tag="PKY09_12310"
     CDS             complement(2306..3298)
                     /locus_tag="PKY09_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNB13676.1"
                     /translation="MTHALIAIIILALLFDFINGFHDAANSIATVVSTKVLSPLSAVI
                     WAASFNFLAYWIFKLKVADTVAKTVTPDSVTLMVVASGLVAAIIWNLLTWWWGIPSSS
                     SHTLIGGFAGAGITNAGSFSALQVDKVMPTVYFIILAPIIGMVMSWIISVITLHLCRK
                     SNVSQVDRVFRKLQLLSSAAYSLGHGGNDAQKVIGIVGAAMLSHGMIADIDHIPEWVP
                     LSCYTAIALGTMFGGWRIVKTMGQRVTKLTPFEGFSAEAAGAFTLFFTQHFKIPVSTT
                     HTITGSIIGVGLTKRISAVRWGVTLQLIWAWILTIPVSGVLAAIIFTIARKVFS"
     gene            complement(3303..>3878)
                     /locus_tag="PKY09_12315"
     CDS             complement(3303..>3878)
                     /locus_tag="PKY09_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNB13677.1"
                     /translation="ALNLQEAGKTMCQLVTSTDADQRKQHIREIERLEHRGDEITHEI
                     FKELSRNFITPFDREDIHRLVSSIDDILDYIHGSSKRIDLYKVKEFSSDMVKLSELLQ
                     TQTEELRRVIYELKNKKNMRNISESLVLINSIENHADDIFDNAVARLFETETNAVEII
                     KTKEILSALETATDMCEDAANVIDSIIVKMA"
BASE COUNT         1316 a          681 c          652 g         1229 t
ORIGIN      
        1 atgaaagttt cactcttggc tgtaatgctc atcatgatac cattggtgtt gttattgatt
       61 gctatagttt ctgctgcaat tttttcgcaa gaagacattg ccttatcctt gagttatggt
      121 tttctgatat tttttggatg gataacagca attattctgg ggatgacatt taaaacactt
      181 ccatttatag tgtggaacaa agtttaccat aaacgttcag gtattggaaa aacaccaaat
      241 ccaaaagatt tatttagtaa cacagtattt aaattaatga gcctggccta tatagtcgga
      301 ttacttttgt ttgtcatagg cagcttaatg attcaccttt tattgataca atgtggtgct
      361 gtactaatac ttacggcagc ttcattatat gtgtttaatg ttttaaaagt agttacacat
      421 aaacctcagc aatgatgaat gtaaaaacaa ataacaactt aaagagtaca gttgccttgg
      481 cagcacttga acatgttgat gacccggaaa taggactcaa cgttgttgac ttaggattga
      541 tttacgaagt taatttcgat gaaaacgaaa aaaagattac gtgtattatg acacttacta
      601 ctgagttttg ccccatgggt gaatctattt ctgaaaatgc acgaaatgca gttcagcagg
      661 tgtttccgga atatttaata gatttgaacc tgacttttga tccctcatgg aatcctgaaa
      721 tgatttcaga agaaggtcga aaatttttag gacgataatt atgctgagtt taagttgtaa
      781 agcaggaatt aaggctgtta tttttctcgg ctcaaagttt gaatcgggcg ataaaaccgg
      841 cattaaagaa atttcaaaac acattaatga aaacgagcat accgttggaa aaatattaca
      901 gaagttggtt aagaacaaca tcattaaaag tgtaaaaggg ccgaatggtg gcttttacat
      961 aacaccaaaa caaaaaaacc aacccatcat aaatattgtt gaagctattg atggcaaaga
     1021 cgtatttaaa caatgtggat tgggattaac aaaatgttca gagtcgcgcc cctgcccttt
     1081 tcacaatgat tttaaacctg tgcgagagtt gtttaaaaat atgtgtatcg aaaaaaaagt
     1141 aagtgacctt tatcagaatg tcaataacgg actttcttac ctggtaggtt gaaaaataat
     1201 aataataacg taagaagaat ttacgcagtc tttgaactaa tggcttcttt caaattaata
     1261 aaaaagttac ctttgatatg atagtgatag gttgatttac gaaaagtaac ttctgttcgt
     1321 aatgaatcaa ttcatttttc agcgtttaca aattccagta tatcacccgg ctgacagtcc
     1381 aacgccttac aaattgcttc taaagtgcta aaacgaacag ctttggcttt gccggttttt
     1441 aagattgaca ggtttgataa tgttaagtca acacgttcag aaagctcgtt aagcgaaact
     1501 tttcgcttgg ccatcataac atctaaattt acaactattg gcatagctta aacagttaaa
     1561 tcgttttctt tctgaagttc aacacctttc ttaaaaatag tagaaattac atacacaaca
     1621 gcagacatca gaataaatgc cttcccatca ttccaaaatt gatataatcg ttcaacatca
     1681 tagccatgat ggtctaaatt tttagttgtc tgtgctgcta tcaaacacat tatcccaatt
     1741 gaaagtgtat aataactgat ttgtgaaatt tgagatgata caaaactgct gaacggtttg
     1801 gacaaatcaa gtttaagtaa taacatgatt acagtataaa ataaaagcac tttcaacaat
     1861 gcaataacaa tgatgaaact atacatactg aaaaaaatcc atttactgcg attatataat
     1921 tcactaaggt ctagcttttg atatagttta cttacaaatt caggattgta aatactgaaa
     1981 acaaaattta caataattcc gcctgcttca atacaaagtc ctacaaaaaa gatccatgct
     2041 atgacgtgta aaacctgaaa gataaatctt gtgttagcca taataaattg tgttttattt
     2101 taggcaaaga taatatatat ttattgtttt tcgataaata tattacttat ttcgataata
     2161 ttgtcttaag ctattgaaaa ccataatcat aaaattcaat aacttcttta acaaccatag
     2221 attgatgtag ttaaagtcaa tccttaaagt aattaggaat acttcttgag gtatttaaaa
     2281 aatctttttt aagaaagtaa ggctactaac taaaaacttt tctggctatt gtaaaaataa
     2341 tagctgcaag cacaccggaa actggtatgg taagaatcca tgcccagatt agttgaagtg
     2401 ttactcccca acgaacggcc gaaattcttt ttgtgagacc aacaccaata atagaacctg
     2461 taatggtatg tgttgtactt accggtattt taaaatgttg tgtaaaaaat aatgtaaatg
     2521 cacctgcagc ctctgcactg aatccttcaa aaggagttag cttggttaca cgttgcccca
     2581 ttgtttttac aattcgccag ccaccaaaca tggttcctaa agcaattgct gtataacaag
     2641 aaagtggaac ccattccggt atatggtcaa tatcagctat cataccatgt gagagcatgg
     2701 cagcgccaac aattccaatt actttttgtg catcgttgcc accatgtcca agactataag
     2761 ctgcagatga tagcaattgt aattttctga aaactcgatc cacttgtgat acatttgatt
     2821 tgcggcataa atgaagtgtg attacagaaa taatccatga cataaccatt ccaataatag
     2881 gcgccaggat tataaaataa accgttggca taactttatc aacttgtaaa gcactaaaac
     2941 tacctgcatt ggtaatacct gcacctgcaa agccaccaat gagagtatgt gatgagctgc
     3001 ttggaattcc ccaccaccat gtcagtaaat tccagatgat agctgcaaca agacctgatg
     3061 caacaaccat caaggtaaca ctatcaggag taaccgtttt tgcaaccgta tctgctacct
     3121 ttagtttaaa aatccaatat gcaagaaaat tgaatgaagc cgcccaaata actgcagaca
     3181 gaggactcaa tacttttgtt gatacaaccg ttgctataga gtttgctgca tcatgaaaac
     3241 cattaataaa atcaaagagt aaggccagaa tgattatagc aataagtgca tgggtcatat
     3301 catcaggcca ttttaactat aatagaatct atgacattgg cagcatcttc acacatatcg
     3361 gttgcggttt ctaatgctga caatatttct tttgttttta taatctcaac agcatttgtt
     3421 tctgtttcaa aaagtcttgc tactgcatta tcaaaaatat cgtctgcatg attttctatt
     3481 gagttaatca gcacaagcga ctctgagata ttgcgcatat tttttttatt cttcagctca
     3541 taaattactc gtctcagctc ttctgtttgt gtctgcagca actcagaaag tttcaccata
     3601 tctgaagaaa actcttttac cttatacaaa tctattcttt ttgatgaacc atgaatataa
     3661 tcaagtatat catctataga acttacaagt ctgtgaatgt cttctctgtc gaaaggggta
     3721 ataaaattac ggctcaactc tttaaatatc tcatgtgtga tttcatcacc acggtgttca
     3781 agccgttcaa tttcacgaat atgttgtttg cgttgatcag catcggtaga ggtaactaac
     3841 tgacacatgg ttttaccggc ttcctgcagg tttaatgc
//
LOCUS       DAOQNP010000142         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_11300_length_3796_cov_5.142743, whole genome shotgun sequence.
ACCESSION   DAOQNP010000142 DAOQNP010000000
VERSION     DAOQNP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11300_length_3796_cov_5.142743"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKY09_12320"
     CDS             <1..923
                     /locus_tag="PKY09_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB13678.1"
                     /translation="PFSLNDESYVMAFIIDITGRRKVQAQMLEQKHQLEEITRELKQT
                     NERLEAKVLDRTKVLEEALNEIEKSRKELSEALEKEKELNELKSRFLSMASHEFRTPL
                     TTILSSASLITAYAEKDQQDKRIRHVERIKSAVNNLNDILSDFLSLSKIEEGKTKTDY
                     EVFNLQNIVSEIINDLATIKKPGQAINYVHTGKNEVLLDPRLIRNIVINILSNAIKFS
                     DEGKLIEIKSNIGDNKTVLEFKDQGIGISKEDQQHLFERFFRGSNATNIQGTGLGLNI
                     VARYVELMNGVIETESELNKGTAFKITIPC"
     gene            926..1816
                     /locus_tag="PKY09_12325"
     CDS             926..1816
                     /locus_tag="PKY09_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNB13679.1"
                     /translation="MNISNPTIFKGHGSAVYCLLSKNKNAFYSGSGDHHLIEWNIENP
                     DKGTVVAILPAGIYSLIAHNHLLIAGCGNGEVHCINTQSATALSKAHLHNSYVFDLCF
                     YNQFLFTAGGDGQIIKTDLQLNKHDSIHLGDFKIRKLLPHPDGQHLIAGCGDGTIRIL
                     NVNTLKEEQIVSAHTKGFSVNALCFTPDKKFLLTGSRDARLNVFDVQQNFKPIDSIAA
                     HNYAIYAIAFNPSGKLMATASRDKKIKIWNDDLTFISRIEEHPAGHKNSVNTLCWLSD
                     TELITTGDDRCIMLWHLSAS"
     gene            complement(1821..2246)
                     /locus_tag="PKY09_12330"
     CDS             complement(1821..2246)
                     /locus_tag="PKY09_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNB13680.1"
                     /translation="MQQENIQAVEEEIIADFELFDDWTQKYEYIIELGQQLPALDEQF
                     KKDEYKIKGCQSSVWLNAYEKDGLLFFEADSDSVFVKGEIALLIKVLSGRKPEEILAA
                     ELKFIDAIGLRQHIAVTRANGLASMIKQIKTYAIAHKEG"
     gene            complement(2249..>3796)
                     /locus_tag="PKY09_12335"
     CDS             complement(2249..>3796)
                     /locus_tag="PKY09_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNB13681.1"
                     /translation="HASSICLNFGIAKRFGGKTNLRFDDTNPVTEDTEYVESIKADIR
                     WLGFEWANEFYASDYFEKLYEFALKLIREGLAYVDDSTAEEIAAMKGTPTEAGKNSPF
                     RNRGVEENLHLFESMRKGEFKEGEKTLRAKIDMASPNMHLRDPLMYRIKHAHHHRTGD
                     KWCIYPMYDFAHGQSDSIENITHSICTLEFEVHKPLYNWFIEKLKIYPSRQFEFARRN
                     ISYTVMSKRKLLQLVNENIVDGWDDPRMPTISGVRRRGYTPESIRNFAEVAGISRREN
                     VTDFNLLESCLREHLNKVAVRTMAVINPLKVVLLNYDESKTEILKAENNPEAVEKTYR
                     DVAFSKEIFIEQDDFMEHAGEGFFRLTPGGMVRLKHAYIIKCENVVKHDDGTIKEIHC
                     TYVAESKSGNDQSGLKVKSVIHWVNAATAVTAEVRLYDKLFTVEAPDAQDDDFKKFIN
                     PQSLQIFSNAKVEPDLLGHEKGERFQFLRLGYFVEDKYSTSAKRIYNRAVTLKDEWAK
                     EQKKQKK"
BASE COUNT         1159 a          766 c          675 g         1196 t
ORIGIN      
        1 gtcctttttc tttaaatgac gaatcgtatg taatggcatt tattattgac ataacaggca
       61 gaagaaaagt acaggcacaa atgcttgaac agaaacatca acttgaagaa attacgcgtg
      121 agttaaaaca aacaaatgaa agactggaag ctaaggttct tgaccgtaca aaagttttgg
      181 aagaagcatt aaatgaaatt gaaaaatcaa gaaaagaatt aagtgaagca ctcgaaaaag
      241 aaaaagaatt gaatgaactc aagtcaaggt ttctgtcaat ggcatcgcat gagtttcgta
      301 caccactaac aactatactt tcatctgcat ctctcattac tgcttatgcc gaaaaagatc
      361 aacaggataa acgaataagg cacgttgaaa gaataaaatc tgctgtcaac aatctgaatg
      421 atattttaag tgactttctt tctctcagta aaattgaaga aggaaaaacc aagaccgact
      481 atgaagtatt caatctgcaa aatattgtaa gtgagattat caatgatttg gctactatca
      541 aaaaaccggg acaagccatt aattatgtgc atacaggaaa aaatgaagtg ttgctcgacc
      601 caaggttgat ccggaatatt gttattaata ttctttccaa tgccattaaa ttttctgatg
      661 aaggtaaact cattgaaata aaatcaaaca ttggtgataa taaaactgta cttgaattta
      721 aagatcaggg cataggaatc agcaaggaag atcagcaaca tttatttgaa cgctttttca
      781 gagggtcgaa tgctaccaac atacaaggta caggtcttgg cttaaatatt gttgcccgat
      841 atgttgagtt gatgaacggt gtaattgaaa ccgaaagtga attaaataaa ggaactgctt
      901 ttaaaattac tattccctgc taataatgaa tatttctaat cctacaattt ttaaagggca
      961 tggcagtgct gtgtattgtc tgttatcaaa aaataaaaat gcattttatt caggcagtgg
     1021 tgaccatcat ctcattgaat ggaatatcga aaatccagac aaaggcactg ttgttgcaat
     1081 tctaccggca ggtatttata gtttaattgc tcataaccat ttactaattg caggctgtgg
     1141 caatggcgaa gtacattgta ttaatacaca aagtgcaact gctttatcaa aagcacattt
     1201 gcataatagt tatgtttttg atttatgttt ttacaatcaa tttctcttta cggcaggtgg
     1261 tgatggacaa ataattaaaa cagacttgca gttgaataaa catgattcca ttcacttagg
     1321 tgactttaaa atcagaaaac ttttaccaca tcctgacggg caacatctta ttgcaggttg
     1381 tggtgatggt acaatcagaa tactaaatgt aaatactttg aaagaagaac aaattgtttc
     1441 tgctcacact aaaggttttt ctgttaatgc actttgtttc acacccgata agaaatttct
     1501 tctgacaggt tcgcgcgatg cacggctcaa tgtattcgat gtacagcaga attttaagcc
     1561 aatagacagt attgctgctc acaactatgc tatttatgct attgcattta acccctcagg
     1621 aaaattaatg gcaacagcca gtcgtgataa aaaaataaaa atctggaatg atgatttaac
     1681 ttttatttct cgtattgaag aacatcctgc aggtcataaa aattctgtaa acacactttg
     1741 ttggctctca gatacagagc taatcactac gggtgatgac agatgcataa tgttatggca
     1801 tcttagtgca tcataaataa ttacccctct ttatgcgcta ttgcgtaagt tttaatttgc
     1861 tttatcatac tggccaaacc attggcgcgt gttacggcaa tatgctgtcg tagtcctatg
     1921 gcatcaataa attttaactc agctgccaga atctcttccg gcttacggcc actaagcact
     1981 ttaatgagta aagcaatctc tcctttcaca aaaacagagt cgctgtcggc ttcaaaaaaa
     2041 agcaaaccat ctttttcata ggcattgagc cacacactgc tctggcagcc tttaatttta
     2101 tactcatctt ttttaaactg ctcgtctaat gccggcagtt gttgccccag ttcaatgatg
     2161 tattcgtact tctgtgtcca atcgtcaaac aattcaaagt ctgcaataat ttcttcctcc
     2221 accgcctgta tattttcctg ctgcatggct atttcttttg tttcttttgt tctttggccc
     2281 actcatcttt taaagtcact gcacggttat atattctttt ggccgaagtg ctgtacttgt
     2341 cttctacaaa atagcccaat cttaaaaact gaaaacgctc tcctttttca tgccccaaca
     2401 aatcgggttc tactttggca ttactaaaaa tttgcaagga ttgcgggttg ataaactttt
     2461 taaaatcatc gtcctgagca tcgggtgcct caacagtaaa taatttatca tacaggcgca
     2521 cctctgctgt gacggcagtg gctgcattta cccagtgtat cacactcttt actttcaagc
     2581 ctgactgatc atttccactt ttactttcag caacgtaggt acaatgaatc tccttgatgg
     2641 ttccatcatc atgcttaacc acattttcgc acttgatgat gtaggcatgt tttagtctca
     2701 ccatgcctcc cggtgttagt ctgaaaaatc cttcaccggc atgctccata aaatcatcct
     2761 gctctataaa aatttctttg ctgaatgcta catcacgata ggttttctct actgcttccg
     2821 gattgttttc tgctttcaaa atttctgtct tactttcatc gtagttcagc aacactacct
     2881 tcaatggatt gataactgcc attgttctta cagcaacttt attcagatgc tcgcgaaggc
     2941 atgattccag caggttaaaa tcagtaacat tttcgcgtct tgaaattcct gccacttcag
     3001 caaagttgcg gatactttca ggtgtataac cacgtctgcg tacaccactg atggtaggca
     3061 tgcgcggatc gtcccaaccg tcaacaatat tttcgtttac caattgcagt aacttacgct
     3121 tactcatgac cgtatagctg atgttgcgtc tggcaaactc aaactgcctg ctgggataaa
     3181 tctttaactt ctcaataaac caattgtaca aaggcttatg tacttcaaac tccaatgtac
     3241 aaatactgtg tgtgatgttt tcaatggagt cactctgtcc gtgtgcaaaa tcgtacatag
     3301 gataaataca ccatttgtcg ccagtgcggt gatgatgggc atgtttgatg cgatacatca
     3361 gtgggtcgcg caggtgcatg ttgggtgatg ccatatctat ttttgcacgc aatgttttct
     3421 cgccttcctt aaattcaccc ttacgcatac tttcaaaaag atgaaggttt tcttcaacac
     3481 cacgattacg gaatggagaa ttttttccgg cttcggtagg cgttcctttc atggctgcaa
     3541 tttcttctgc tgttgaatca tctacgtaag ctaatccttc acgaataagt tttaaggcaa
     3601 actcatacaa tttttcaaaa taatcactgg cataaaattc attggcccat tcaaagccca
     3661 accaacgtat atctgcttta atgctctcta catattctgt atcttctgta acggggttgg
     3721 tatcatcaaa ccgcagattg gttttgcctc caaatcgctt tgctatgcca aaattcagac
     3781 agatggacga ggcatg
//
LOCUS       DAOQNP010000143         3663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_12009_length_3663_cov_10.973115, whole genome shotgun
            sequence.
ACCESSION   DAOQNP010000143 DAOQNP010000000
VERSION     DAOQNP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12009_length_3663_cov_10.973115"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="PKY09_12340"
     CDS             <1..1522
                     /locus_tag="PKY09_12340"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13682.1"
                     /translation="KNGIELTGCDTSTVSCNVVSGRYPAQTYQNKGINGSMNHQCYFS
                     CNITDSTYRGFNFVSSNMGTKFRGNEMKKHYIGLYLDNTAVIDTQSHAGNMWTGAYNS
                     TYGAWNENDSSAFNLSQSLFIVDPILGAFYNPNIPLGINPGEPNDQGWFQPDTSGHTF
                     ECDGLLLCVDNPAERGEGSDNLKEAIAADNTITSEYIEESKSTAKMFLYEDLKNDSAA
                     YSGNTVLMQFLSNNVNTTFGKLNNVKQLMKEANTLDYSNVMLLKEKDSLINIHLSNMH
                     LLDSIAASDSTINNIIARENEIAEINTLKQDISNITSSDNTNKAAKLSETITWNNAVQ
                     PNEIPEYNLKQVNDINIIYEHTESKDSIAQNYGTLLNIAVQCPQSGGIAVYIARSLLA
                     LFNDSIIYDDDNVCFQQGYYRLSNELKNNRAFIEAVLIPNPASNYADVVLNKTFEGIC
                     KVKITDIYSKVIFEQSFDCGKKQFRINTTGFLNGIYNVQIIINDNAQSLIKLIIVK"
     gene            1519..3006
                     /locus_tag="PKY09_12345"
     CDS             1519..3006
                     /locus_tag="PKY09_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13683.1"
                     /translation="MKKLILVLFIPLIVQGQINNTWLIGYGNWAVRARIIFETNNYIL
                     TQESRQMGFQGAEATISDDNGNFLMSSNGVWIADATGDTMINGGGLNPSWDVNAHPNG
                     ILTENSNMFLPFPNDSNKYILIHHALFSDISTYSYWGGVHKSVIDITANGGLGAVIEK
                     NDTLIYDEISGGITACRHANGRDWWVIVQKDSSDVVYKILLTPNGVDTITTQHLGYAQ
                     FFNGNVSQITFSQDGKKFIQNNYHYVAGNMHPSFVILADFDRCTGMFSNTQTIQLTQD
                     SYLWGLAFSPSGKYAYACSSLYLFQIDTDSLTVDTVATYDGFYSPYSWCCATTFWTMY
                     LAANGKIYMTSGNGVQHIHEMNYPDSASIACDLQQHAINLGVWSFRAVPNHPNYYLGC
                     DTTQTTCPCLITGINEIKENDFKFSISPNPSNGNFKIMYLLPQNSKGTFEVFDITGKK
                     VFNYNLPQWSTLQNFDLSFLGNGIYHCSITSNNQRVNKKLVILNE"
     gene            3011..>3663
                     /locus_tag="PKY09_12350"
     CDS             3011..>3663
                     /locus_tag="PKY09_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13684.1"
                     /translation="MLCAGDPTKVRTVNFPAYAGQVLRKKKIYGWLCWCLFLWFVSFG
                     QAKEMNREVTLASMQCATYTVSGYFTTQGSCFYNVASVTSPMGTTWKDSVCVSVVSLC
                     STTDTTFADSTFSGNAIINNQSIFLANRFYINGNLTLNSCTVYTAAGAQLIVQNGGIL
                     TLNNTTLQACDTMWRGIEVLENGTTILNSSSIADAENGILLRNKGIIKSVNSNFYDN"
BASE COUNT         1220 a          622 c          722 g         1099 t
ORIGIN      
        1 caaaaatggt attgaactga caggttgcga cacttcaaca gtgagttgta atgttgtaag
       61 tggacgttat ccggcacaga cttatcaaaa taagggaata aacggtagca tgaaccatca
      121 gtgttatttt agctgtaata ttacagactc aacttacagg ggttttaatt ttgtgagcag
      181 taacatgggc actaaattca gaggtaatga aatgaagaag cactacatag gtttgtattt
      241 ggataacacg gcagttatag acacacaaag ccatgcaggt aatatgtgga caggagcata
      301 taacagcacc tatggtgctt ggaatgaaaa tgactcttct gcttttaacc tctcgcaatc
      361 attatttatc gttgacccta tattaggtgc tttttacaac cccaatatac ctctgggaat
      421 aaacccagga gagccaaatg accagggatg gtttcaacct gatacaagtg ggcatacctt
      481 tgagtgtgat ggcttattgc tttgtgttga taatcctgct gaacgtggtg aaggctcaga
      541 taatttaaaa gaagctattg ctgccgataa cacaataaca tctgagtata tcgaagaatc
      601 aaaaagcact gcaaaaatgt ttttatatga ggatttgaaa aatgattctg ccgcctacag
      661 tggtaatacc gttttaatgc agtttctaag taataacgta aatacaacat tcggtaaatt
      721 gaataatgta aagcaactaa tgaaagaagc taatacgtta gattattcaa atgtaatgtt
      781 gctaaaagaa aaagatagcc ttataaatat tcatttgagt aatatgcatt tattagatag
      841 cattgctgcc tctgacagta ccattaataa tataatagca agagaaaatg aaatagcaga
      901 aattaatact ttgaagcaag atattagcaa cattacatcc tctgataata caaataaagc
      961 ggctaaactt agcgaaacga taacatggaa caatgctgtg cagccgaatg aaattcctga
     1021 atacaattta aagcaagtaa atgatataaa tattatttac gagcatacag aaagcaaaga
     1081 ttccattgct caaaactatg gcacattatt gaatatcgct gtgcagtgtc cacagagtgg
     1141 cggaattgca gtttacattg ctcgtagttt attagcactt tttaatgact caataatata
     1201 tgacgatgat aatgtttgtt ttcagcaagg ttattacaga ttatcaaatg agttaaaaaa
     1261 taatagagct tttattgaag cagttttaat tccaaaccct gcaagtaatt atgcagatgt
     1321 ggttttaaat aaaacatttg agggtatttg taaagttaag ataaccgata tttattcaaa
     1381 ggtgatattt gagcagtcat ttgattgcgg taaaaagcaa ttccgaataa atacaacagg
     1441 ttttttaaat ggaatttata atgtgcaaat tatcataaat gataatgctc agagtttgat
     1501 taaactcata attgtaaaat gaaaaagcta atacttgtat tgttcatacc cttgattgtg
     1561 caagggcaaa ttaacaatac atggctgata ggctatggta attgggctgt tcgtgcaaga
     1621 attatttttg aaacaaataa ttacattttg acgcaagaaa gtcgccaaat ggggtttcaa
     1681 ggagcagaag ctaccatcag tgatgataat ggtaatttct taatgagcag taatggcgtt
     1741 tggatagccg atgccactgg tgataccatg attaacggag gtgggctaaa tccaagttgg
     1801 gatgtaaatg ctcaccctaa tggaatatta acagaaaaca gtaatatgtt tcttccgttt
     1861 cctaatgaca gtaataaata tattctaatt catcacgcat tgttttctga tatttctact
     1921 tattcttatt ggggtggagt tcataaatcc gtaattgata taacagctaa cggaggatta
     1981 ggagcggtaa tagaaaagaa cgacacgtta atttatgacg aaataagcgg aggtattaca
     2041 gcttgccgtc atgctaatgg cagggactgg tgggtaattg ttcaaaaaga tagcagcgat
     2101 gtagtgtata aaattcttct tacgcctaat ggtgttgaca ctattactac gcagcatctt
     2161 ggttatgccc aattttttaa tggcaatgta tcacaaatca cattttctca ggacggtaaa
     2221 aaatttattc aaaataacta tcattatgta gcaggcaata tgcaccctag ttttgtaatt
     2281 cttgccgatt ttgataggtg tacaggtatg ttttcaaaca cacaaaccat acaattaaca
     2341 caagatagct acttgtgggg gcttgcattt tctccttcgg gcaaatatgc ttatgcttgt
     2401 tcgtcattat acctatttca aatagacacc gatagtttaa cggttgatac cgtggctacc
     2461 tatgatgggt tttattctcc atattcatgg tgctgcgcaa ctactttctg gacaatgtat
     2521 ttagccgcta acggaaaaat ttatatgact tccggcaatg gtgttcagca tatacatgaa
     2581 atgaattatc cagatagtgc aagcatagct tgcgatttgc agcagcatgc catcaattta
     2641 ggtgtgtgga gttttcgtgc tgtccccaac caccccaact actacctcgg ctgcgatacc
     2701 acacaaacaa cttgcccatg tttgataact ggcataaatg aaattaagga gaatgatttt
     2761 aagttttcaa tctcccccaa cccgtccaat ggcaatttta aaatcatgta cttgttaccg
     2821 cagaatagta agggaacttt tgaagtgttt gatataacag gaaaaaaagt ttttaattac
     2881 aatcttccgc aatggagtac gttacaaaat tttgatttga gttttttggg taatggtatt
     2941 tatcattgca gcatcaccag caacaaccag cgtgtaaaca aaaaattggt aattctaaac
     3001 gagtagtacc atgttgtgcg caggcgatcc aacaaaggtg cgaaccgtaa attttccagc
     3061 ttatgcaggg caggtattgc gtaaaaagaa aatttacggt tggctttgtt ggtgtctttt
     3121 tctttggttc gtttcttttg gacaagcaaa agaaatgaac agagaagtaa ccctcgcctc
     3181 catgcaatgc gccacgtaca ccgtaagcgg ttattttact acacaaggca gttgttttta
     3241 caatgtagca agtgttactt cgccaatggg cactacgtgg aaggatagtg tttgtgtgag
     3301 tgtggtaagt ctttgctcca ccaccgacac tacttttgct gatagtacct tctccggcaa
     3361 tgcaatcatt aacaaccaat ccatattttt ggccaaccgc ttttatatta acggcaattt
     3421 aacgctgaac agttgtactg tttacacagc agcaggggcg cagctaattg tacaaaacgg
     3481 aggtatctta acactcaaca acacaacttt acaagcatgt gataccatgt ggagaggaat
     3541 agaagtattg gaaaacggaa caacgatact aaacagcagt agcattgctg atgcagaaaa
     3601 cggcatcttg ttgcgcaaca aaggcattat aaaatctgta aacagtaact tctacgacaa
     3661 cat
//
LOCUS       DAOQNP010000144         3432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_13460_length_3432_cov_9.381404, whole genome shotgun sequence.
ACCESSION   DAOQNP010000144 DAOQNP010000000
VERSION     DAOQNP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13460_length_3432_cov_9.381404"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="PKY09_12355"
     CDS             complement(<1..1463)
                     /locus_tag="PKY09_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13685.1"
                     /translation="MRKMIAHKSLVWVFHTFVFVVCYCSAYSQMNNTWLIGNQPLANV
                     LKARMVFTDTSYSFNTELRKMTFEGTEATISDANGNLLMSSNGVWIADATGDTMMNGS
                     GLNPSWDVNAHPRGLLNDGSNIFLPLPNDSNKYILIHEAYFDTVFNYSYGGGVHLSKI
                     DIVANGGLGAVISKNDTLIDDKLTMGLTACRHANGRDWWVIAQKDSSDIVYKILITPN
                     GVDTITTQNLGYNQFFYGNGSLIHFSQDGTKFIQSNYHYVAGNLHPSFVILADFDRCT
                     GMFSNTQTIQLTQDSYLWGLAFSPSGKYAYACSSLYLFQINTDSLTVDTVATYDGFYS
                     PYSWCCATTFWTMYLAANGKIYMTSGNGVQHIHEMNYPDSTGVACDLQQHAINLGVWS
                     FRAVPNHPNYYLGCDTTQTTCPCLVTGIKELSQHDFRFKIMPNPTNGNFKIMYLLPQN
                     SKGIFEVFDITGKKVFNYNLPQWSTLQNFDLSFLGNG"
     gene            complement(1460..3058)
                     /locus_tag="PKY09_12360"
     CDS             complement(1460..3058)
                     /locus_tag="PKY09_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13686.1"
                     /translation="MVNDSAIVANNAKNANVINNFVELSGNTKNGISLTGCDSSTVSC
                     NTVIGRYPAVNYQNRGIYGSMNQKCYISCNITDSLYKGVHFSSSNIATKFRGNEMKKH
                     YIGLYLDNTAVIDTQSHAGNRWTGTYNSTYGAWNENDSSFTSLRQSLFIVDPVLGAFF
                     NPNIPMGINPGEPNDQGWFDPIVGGDTYSCTGYLLCADKPIERSQGSGDLKEAIATDD
                     VLTTDYIEESKIIAKIFLYEEVKKDSAEYSSNSVLMQFINDNTSGTLGKLSNTKQYIK
                     EAFSITSLTELLLKERDSLLNIHLNYVLSLDSLVAADSNITNIQIREAEIDEIDILKQ
                     EINNIIELSKDTQKLYLAKAVNESIQPNEIPEYNLKQVNSINLLYEKTNSKDSISGYY
                     ATLLNIAEQCPQSGGMAVYVARSLVALFNDTVVYEDDNVCLQQGYFRRAHKANKKSIP
                     YDITIVPNPADESTQIILNKKQEGVCKVNITDAYSKCVYNNEFDCKTQQLTVSTKWLV
                     QGIYFVEIKISGSTVKTAKLIITR"
     gene            complement(3076..>3432)
                     /locus_tag="PKY09_12365"
     CDS             complement(3076..>3432)
                     /locus_tag="PKY09_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13687.1"
                     /translation="WSFRAVPNHPNYYLGCDTTSGCPCLITGINDIKQYDFKFSISPN
                     PSNGNFKIMYLLPQNSKGVLEVFDLTGKKVFSYNLPQWSTLQNFDLSFLSNGVYHCSI
                     KSGNYYVQKKLAIIRY"
BASE COUNT         1028 a          676 c          582 g         1146 t
ORIGIN      
        1 ataccattac ccaaaaaact caaatcaaaa ttttgtaacg tactccattg tggaaggttg
       61 taattaaaaa cttttttgcc tgtaatatca aaaacctcaa aaatgccctt actattctgc
      121 ggtaacaagt acatgatttt gaaattaccg tttgtgggat tgggcatgat tttaaaccta
      181 aaatcatgtt gtgaaagttc ttttattcct gttactaaac aggggcaagt tgtttgagtc
      241 gtatcacaac ctaaataata attagggtga ttaggaacag cacgaaaact ccacacccct
      301 aagttaatgg cgtgttgctg taaatcacac gcaacacctg tactgtcagg ataattcatt
      361 tcatgtatat gctgaacacc attgcctgat gtcatgtaaa tctttccgtt ggcggctaga
      421 tacattgtcc agaaagttgt tgcgcaacac catgaatagg gagaataaaa accatcatag
      481 gtagctacgg tatcaaccgt taaactatcg gtatttatct gaaacaaata taatgatgag
      541 caagcataag catatttgcc cgagggagaa aatgccagcc cccacaagta gctgtcttgt
      601 gttaattgta tggtttgtgt gttggaaaac atacctgtgc acctgtcgaa atcggcaaga
      661 ataacaaagc tggggtgcag gttgcctgct acgtaatgat agtttgactg aataaatttg
      721 gtaccatctt gtgaaaaatg tatcagagaa ccattaccat aaaagaattg attataacca
      781 agattctgtg tggttattgt gtccacacca ttgggggtaa tcaaaatttt atacactatg
      841 tcgctgctgt ctttttgagc tatcacccac cagtctctac cattggcatg gcgacaagcc
      901 gtaagaccca tagttaactt atcatcaatt aaagtatcat tcttactaat aactgcacct
      961 agtccaccgt ttgcaacaat gtcaatttta gatagatgca cccctccacc atacgaataa
     1021 ttaaaaacag tatcaaagta agcctcatgg atgagaatat atttattgct gtcattaggt
     1081 agaggcaaaa aaatattact gccatcattt aacaaccctc ttggatgcgc attcacatcc
     1141 caactcgggt ttaatccact gccattcatc atggtatcgc cagtggcatc ggctatccat
     1201 actccgttgc tactcatcag caggttaccg ttggcatcgc tgatggtggc ttcggtgcct
     1261 tcgaatgtca tttttcttag ttctgtgtta aagctatagg aagtgtctgt aaaaaccatt
     1321 cttgccttta atacatttgc caaaggttgg ttcccgatca accaagtatt gttcatttgt
     1381 gaataggcgc tacagtagca cactacaaaa acaaatgtat ggaataccca cactagcgat
     1441 ttatgtgcta tcatctttct catcgtgtaa taatcaactt agctgtttta acagtactac
     1501 cgcttatttt gatttctaca aaatagatgc cttgcaccaa ccatttggtt gaaactgtta
     1561 attgttgtgt tttacaatca aattcgttat tgtaaacaca ttttgaataa gcatctgtga
     1621 tatttacttt gcacactccc tcttgttttt tatttagtat tatttgtgtg ctttcatcag
     1681 ccgggtttgg aacgatagta atgtcatagg ggatgctttt tttatttgct ttatgagccc
     1741 tgcggaaata gccttgctgc aagcaaacat tatcatcttc ataaacaaca gtatcattaa
     1801 acaacgctac taagctacgt gcaacataaa ctgccatacc accactttga gggcattgct
     1861 cggcaatgtt taacaatgtt gcataatagc cgctaatgga atccttactg tttgttttct
     1921 cataaagtaa attaatgcta tttacttgtt ttaaattgta ttcgggaatt tcatttggtt
     1981 gaatactctc atttactgct tttgccaaat acagtttctg agtatccttc gatagttcta
     2041 ttatattgtt aatttcttgc tttaaaatat caatttcatc aatctcagct tctctgattt
     2101 gtatattagt aatgttgctg tcggcagcaa ctaaactatc taatgacaat acataattca
     2161 aatggatatt taataagctg tccctttctt ttaaaagtaa ttcagtaaga gaagtaatag
     2221 agaaagcttc tttaatatat tgttttgtat tgcttaactt acctaaagtg cctgaggtgt
     2281 tgtcattaat aaattgcatt agtacgctat tgctgctata ttctgccgaa tcctttttaa
     2341 cttcttcata caaaaatatt tttgcaataa tcttcgactc ttcaatgtag tcagttgtca
     2401 atacatcatc agttgctatt gcttctttta aatcaccaga tccctgactg cgttcaatag
     2461 gcttgtcagc acataataaa tatcctgtac aactgtaggt gtctccacct acaattggat
     2521 cgaaccaccc ctgatcattc ggctctcctg ggtttattcc catgggtata ttggggttga
     2581 aaaaagcacc taacacaggg tcaacgatga ataaggattg ccttaaagaa gtaaacgaag
     2641 aatcattctc attccaagca ccataggtgc tgttatatgt tcctgtccac ctattgccag
     2701 catgactttg tgtatctatc actgccgtat tatccaagta caaaccaatg tagtgctttt
     2761 tcatttcatt acctctgaat ttggtggcta tattactgct gctgaaatgt actcctttat
     2821 acaaactgtc tgtaatatta caactgatgt agcatttttg attcatgctt ccgtatattc
     2881 ctctgttctg ataatttacc gcaggatagc gtccgattac agtgttgcag cttaccgtgc
     2941 tgctatcgca accggttaaa gaaatgccgt ttttggtatt gcctgatagt tcaacaaagt
     3001 tatttatcac attggcattt tttgcattgt tagctactat agcgctgtcg tttaccactt
     3061 caatattcgg attgtttaat atctgataat cgcaagtttc ttttgtacat aataattgcc
     3121 cgatttaatt gaacaatgat aaacgccatt acttaaaaaa ctcaaatcaa aattttgtaa
     3181 cgtactccat tgcggcaggt tgtaagaaaa aactttttta cctgttaaat caaacacttc
     3241 taaaacacct ttactattct gcggcaacaa atacatgatt ttgaaattgc cgttactagg
     3301 gttgggtgaa atagaaaatt taaaatcata ttgcttaata tcattaatac ctgttatcaa
     3361 acaagggcaa ccgcttgtag tatcgcaccc caaataataa ttagggtggt tgggtacagc
     3421 cctaaaactc ca
//
LOCUS       DAOQNP010000145         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_14317_length_3312_cov_6.224133, whole genome shotgun sequence.
ACCESSION   DAOQNP010000145 DAOQNP010000000
VERSION     DAOQNP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14317_length_3312_cov_6.224133"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKY09_12370"
     CDS             <1..269
                     /locus_tag="PKY09_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNB13688.1"
                     /translation="NLFLIIKESLHNSIKHARAKETVLIYEKTENGHRLSIRDNGMGF
                     SEQDIQTKKGNGLSNLQKRAIKINSRVNIQSEPGKGTVVIIDFI"
     gene            315..929
                     /locus_tag="PKY09_12375"
     CDS             315..929
                     /locus_tag="PKY09_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNB13689.1"
                     /translation="MVKVCIVEDIHEIREGFRGVLNLSGEFTCCGSYASAEDAIKEIP
                     ELNPEIIIMDINLPGISGIEAINLLKQQNCKAQFLIFTVYDDDEIIFDALQTGASGYL
                     LKKTAPARFLEALKEMHEGGSPMSPTIARKVISSFQPGLKTNTVLTPRETEILTLLSK
                     GLLYKEISEQLFISTGTVKQHIHKIYEKLHVQNKTEAINKFLNK"
     gene            complement(1074..1655)
                     /locus_tag="PKY09_12380"
     CDS             complement(1074..1655)
                     /locus_tag="PKY09_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13690.1"
                     /translation="MKKLICAALIAAGITTMTTTTKAQGGDPPFVKGSNSIGLSIGFG
                     DYYDYGSVYYGSSWTVLPSINFHFDHGTIAEVGPGTIGIGGIIGIKNRYYKYSNGDKY
                     TDNSIIVGVRGTYHLTLLADRNNKFDPYGGVLLGARIRTRNDHYYDDKRNNVYPTTGL
                     FVGAKYNFVENFGAFAELGYDISFFKIGLNLNF"
     gene            complement(1805..>3312)
                     /locus_tag="PKY09_12385"
     CDS             complement(1805..>3312)
                     /locus_tag="PKY09_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13691.1"
                     /translation="PGINYLWISTDIKTSAMPAAVIDAELTSLTVNGSSLLPLLASAP
                     GNKKIRNNTVLFSVRSGNFNDLSLWNSQRDGKGTTPANIPQNTCFFIQSGHEFTLSES
                     DTSSIAVVENNGKLNCDKQYALKEVCVEQGAIFQLNENENKSGNLELLILKDGSNYIH
                     NSTGSFASEHAQLSNTSQVVFMKYGNHSFGKDVAWGNVMFDAAQGVDANIGKAFRNIY
                     GNLELRNTGKGSLFTEGVNIMSVKGDFIVSGGSFEGVRNVNSKLVLNVGGSFKVLAGN
                     ISDVVNKTTPGARTTFNLYGDVMLLGGKIMLNNADDDGSFINIMGRENPQVRWMQTDK
                     AQVELCNVAIKPEKELFLKGDKIGDIPAGRLFSVERNAKLWCAALSVTGQGRFSLADN
                     ATLGIGHSKGINSEKSEGNILTKERQFSSGANYIYYMAVSPQTMGEFVTFPKEKTIRN
                     LVIKKDSPMQQVNLASDLFVSDQVKVSMGDFNRGSFKLQLSDVSESLHRLN"
BASE COUNT         1066 a          677 c          557 g         1012 t
ORIGIN      
        1 gaaatttgtt tcttataata aaagaatcat tgcataacag tataaaacat gcaagggcta
       61 aagaaactgt gttaatatat gaaaagaccg aaaacgggca tcgccttagc atacgtgata
      121 acggtatggg attttctgaa caggatattc aaaccaaaaa gggcaatggg ttaagcaatt
      181 tacaaaaaag agcgataaag attaatagcc gtgtgaatat tcagtcggag ccggggaaag
      241 gaacagtagt aattattgac tttatctaac aaaagttaca tttgtggaga taacggagta
      301 cgtattattg cataatggta aaagtttgta ttgttgaaga catacatgaa atcagagaag
      361 gttttagagg ggtcttgaat ctttcagggg agttcacatg ttgtggttca tatgccagtg
      421 ctgaagatgc tattaaagaa attcctgagc ttaacccaga aattatcatc atggatatta
      481 atcttccggg tataagcggc attgaagcta taaatctgct taaacaacaa aactgcaaag
      541 ctcagttctt gatatttact gtttatgatg atgatgaaat aatttttgat gcactacaga
      601 caggtgcaag cggctacctc ttgaaaaaaa ctgcacctgc ccgttttctt gaagcattaa
      661 aagaaatgca tgaaggcgga tcgcctatga gccctaccat tgcgcggaaa gtaatcagtt
      721 cgtttcaacc tggattaaaa acaaataccg tattaacacc acgcgaaaca gaaatactca
      781 cactgctttc taaaggatta ttgtacaaag aaatttcaga acaacttttt ataagcacag
      841 gaacggtaaa acagcatatt cataagattt acgaaaaact ccatgttcag aataaaacag
      901 aagcgattaa taagtttttg aataaataat agtgtttaaa gacagcggtg gtttaataga
      961 ccctccgtac tcccaaagtc tcccactttt caggggactt tgaaataatg gacaaaaaat
     1021 aagtaacaaa aaaaggcgac ctttcggtcg ccttgacaac actaatttta tttttagaaa
     1081 ttaagattca agcctatctt aaagaaagaa atatcataac caagttctgc aaaggcacca
     1141 aaattctcta caaaattata tttagcacca acgaataatc ctgttgttgg atatacatta
     1201 ttacgtttgt catcgtaata atggtcgttt ctggttctga tacgtgctcc caacaatacg
     1261 ccaccataag gatcgaattt attattccta tcagctaata aagtaaggtg atatgttcca
     1321 cgaacaccta caattatgct gttgtcagtg tatttatcgc cattactata tttataataa
     1381 cggtttttaa tcccaataat tccacctatg ccaattgtac cgggtccaac ttctgcaatt
     1441 gttccatgat caaaatgaaa attaattgat ggaagcactg tccagctact gccgtagtaa
     1501 acacttccgt aatcgtaata atcgccaaag cctattgaca atccaataga gtttgaacct
     1561 ttaacaaaag gaggatcgcc accctgcgct tttgtcgttg ttgtcattgt tgtaattcct
     1621 gctgcaatca aggcagcgca aattagtttt ttcatttttc tttcgtttaa ttctatttat
     1681 taggaagcaa aactaaatta ttaccatgaa gacaggaaaa atttaagatg aatttattaa
     1741 ccatcagaaa ctaaaaacca ccttagctac actaaggtgg ttacacaatt ttttttaaca
     1801 attatcaatt gagccgatgc aatgactcac tgacatctga tagttgtaat ttaaaacttc
     1861 cgcggttgaa atctcccatg ctcactttaa cctgatcgct cacaaataaa tctgatgcca
     1921 gatttacttg ttgcataggg ctgtctttct taatcaccaa attacgaatg gttttttctt
     1981 taggaaatgt aacaaactcg cccatagttt ggggtgatac tgccatataa tatatatagt
     2041 ttgcaccaga gctaaactgc ctttcttttg ttaaaatatt tccttcgctt ttttctgaat
     2101 ttattccttt actgtggcct ataccaaggg ttgcattatc ggccagacta aacctgccct
     2161 gcccggtcac actcagtgcg gcacaccaca gtttggcatt tcgctctact gaaaacagcc
     2221 ttcctgccgg tatgtcgcca atcttatctc ctttcagaaa taattctttt tcgggtttaa
     2281 tggcaacatt acacaactcc acctgtgcct tatcggtctg catccatcgt acctgcggat
     2341 tttcacgacc cattatattt ataaacgatc catcgtcatc tgcattgttc aacattattt
     2401 tcccgccaag caacataaca tcgccataaa gattaaaagt tgtccgcgct ccgggtgtgg
     2461 ttttgtttac aacatcactt atgtttccgg caagcacctt aaagctaccg cctacattta
     2521 aaaccaattt actgtttaca ttcctgacac cctcaaaact tcctcccgaa acaataaaat
     2581 ctcctttaac agacatgata tttacccctt cagtaaacaa agaaccctta ccggtattgc
     2641 gcagttcgag gtttccataa atatttctga atgccttacc aatatttgca tcaacgcctt
     2701 gtgccgcatc aaacatcaca ttgccccatg caacatcttt tccaaacgaa tggttgccat
     2761 acttcataaa caccacttgt gatgtatttg ataattgtgc atgctcagaa gcaaaactcc
     2821 cggtgctatt atgaatataa tttgagccat ccttcaaaat caaaagctct aaattgcccg
     2881 atttattttc attttcatta agttgaaaaa tggcaccttg ttcaacacaa acctctttta
     2941 atgcatattg tttatcacaa ttcaatttgc cattattctc taccacagca attgaagagg
     3001 tgtcgctttc acttaatgta aactcatgtc cgctttgaat aaaaaagcag gtgttttgag
     3061 gaatatttgc cggtgtggtg cctttaccat cacgctgtga attccataaa gacaaatcgt
     3121 tgaaattacc tgaacgcaca gagaaaagca ctgtattatt tcttattttc ttatttcccg
     3181 gtgctgatgc aagcagtggc aataaagatg aaccgttcac cgtgagtgaa gtaagttctg
     3241 catcaataac agcagcaggc attgccgaag tcttaatgtc tgtactaatc cacaaataat
     3301 ttatgcccgg ag
//
LOCUS       DAOQNP010000146         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_14994_length_3229_cov_8.827347, whole genome shotgun sequence.
ACCESSION   DAOQNP010000146 DAOQNP010000000
VERSION     DAOQNP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14994_length_3229_cov_8.827347"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKY09_12390"
     CDS             complement(<1..509)
                     /locus_tag="PKY09_12390"
                     /inference="COORDINATES: protein motif:HMM:NF026223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-repeat neuraminidase N-terminal
                     domain-containing protein"
                     /protein_id="HNB13692.1"
                     /translation="MFKRKKLLSQTPSMRTNLRMAMYIGSASLSGLVVLLLIVFNISK
                     REISKAEYSGMQVINTEVFQNTDVVYRGSVNNPIIGIMIDVKGYKTAVKLNSITFSLK
                     GTTQPYFKQVENIKVWSSGQAKTFSGLKQLYQSIAPSEGNVRIDINSMLSPGINYLWI
                     STDIKTSAMP"
     gene            684..1202
                     /locus_tag="PKY09_12395"
     CDS             684..1202
                     /locus_tag="PKY09_12395"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNB13693.1"
                     /translation="MSLKKIMFISGMPGLYQLVAQSRNGFIAESLADKKRSHVSNNQQ
                     VSMLSDISIFTTADDVKLAEVFRKMTALDEKLTKIDLNADGSVILSSFEKILPEFDKD
                     RVYTSDIKKVFKWYNLLKDKIDLAATEETTEDAATENTSPIEAEKTEAVEETETKPKA
                     KRKTKKAENDKA"
     gene            1206..2933
                     /locus_tag="PKY09_12400"
     CDS             1206..2933
                     /locus_tag="PKY09_12400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNB13694.1"
                     /translation="MSLTTENIGKANRHFLKSDFSVTSWDDLKPYYENLISRTINSKE
                     DLIEWLLDRSELESALQENLAWRYIRMSCDNTNEKLQQDYNYYVREIDPNISPEDNKL
                     NEKFIACPFSELLSETDYRNYLRNVKQRISLFRKENIPLLAELAEKQQLFGQISGAQT
                     ITYNNQEHTLQQASVWLKNTDRAIRETVYKKITARRLEDKKQLDDLYTNLIQLRHQVA
                     LNAGFKNFRDYSFAALERFDYSVEDCYRFHESIELEIKPVINQLEEYRKTKLDVAHLM
                     PWDLAVDPTGKPPIKPFSNTQEMVEKTILCLKEIDPEFANCITLLDEMNRFDLDSRKG
                     KSPGGYNYPLYETGVPFIFMNSTGQLRDLVTMVHESGHAVHAVLAHNLPFHEMRSCPS
                     EVAELASMSMELLTMEHWHHFISDEKELKRAKLEHLTSLIEILPWIAIVDAFQQWVYL
                     HPEHSVTEREQQFVQLYNRFSGTVVDWQGFENVKENLWQKQIHIFDSPFYYIEYGMAQ
                     LGAIALWKNYKTDATATIRNYKKALALGYTKSIGEIYKAAGVSFDFSATYIKELSQFV
                     QSEIKELQK"
     gene            3033..>3229
                     /locus_tag="PKY09_12405"
     CDS             3033..>3229
                     /locus_tag="PKY09_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNB13695.1"
                     /translation="MQVKSTYLNIWQIAYPIMLASVAQMLVGLTDTAFLARVGEVELG
                     ASAIAGVFYFVLVMLGMALSIG"
BASE COUNT         1099 a          579 c          626 g          925 t
ORIGIN      
        1 ggcattgccg aagtcttaat gtctgtacta atccacaaat aatttatgcc cggagaaagc
       61 atgctgttaa tatcaattcg aacattgcct tcagaaggtg ctatggactg ataaagttgt
      121 ttgagaccgg aaaatgtttt tgcctgacct gatgaccaaa ctttaatgtt ctcaacctgc
      181 ttgaaataag gttgtgtggt tcctttcaat gaaaatgtta ttgaattaag tttaacagca
      241 gtcttatatc cttttacgtc aatcataatt ccaattatag gattgttaac actaccgcga
      301 taaacaacat ctgtattctg aaaaacttct gtattaataa cctgcatgcc gctatactca
      361 gctttagaaa tttctcgttt tgaaatatta aaaacaatga gcaatagcac cacgagtccc
      421 gacaaggaag cagagccaat atacatggcc atgcgcaggt tggtgcgcat agaaggagtt
      481 tgacttaata atttttttcg tttaaacatg attagaatat taacatctta acctgacctt
      541 atactgtata taattaaata agtttcaata gcatataccc acaactgcct gaatgcaggt
      601 tttatgaatg tggaaaaaat caggcccaca gccagttata aaattttaac tttgtcgcat
      661 tattaaaatg aatattctaa aaaatgagtt tgaaaaaaat catgtttatc tccggtatgc
      721 ccggtttgta ccaattagtt gcacaaagcc gcaatggttt tattgcagaa tcgcttgctg
      781 ataaaaaacg atcgcatgtc agcaacaacc agcaagtaag tatgctttcc gacatcagta
      841 tttttactac cgcagacgat gttaagttgg cagaagtttt ccgaaaaatg actgcattag
      901 atgagaaatt gactaagata gatttgaatg cagatggatc tgttatttta tcgtcatttg
      961 aaaaaattct accggaattt gataaagaca gagtttatac ctcagacata aaaaaagtgt
     1021 ttaagtggta taacctgctt aaagacaaaa ttgatttagc agcaacagaa gaaactacag
     1081 aagatgcagc aacagaaaat acttctccca ttgaggcaga gaaaactgaa gctgttgaag
     1141 aaactgaaac aaaaccaaaa gcaaagcgca aaacaaaaaa agcagaaaac gataaagcct
     1201 gatttatgtc tctgacaaca gaaaatattg gaaaggcaaa tcgccatttt cttaaaagtg
     1261 attttagtgt aacatcgtgg gatgatttga aaccttacta tgaaaacctg atttcgcgta
     1321 caataaactc taaggaggat ttaattgaat ggctgctgga cagaagcgaa ttggaatcgg
     1381 cactgcagga gaatttagcg tggcgttata tcaggatgag ttgcgacaat acaaatgaaa
     1441 aactgcaaca ggattataat tattatgtaa gagaaataga cccgaacatt tctcctgaag
     1501 ataataagct gaacgaaaaa tttattgcct gccctttttc agagttgctt tctgaaacag
     1561 actacagaaa ctatctgcgc aatgtaaaac aacgtatttc tttattcaga aaagaaaata
     1621 ttccactgct tgcagaactt gcagaaaaac aacaattatt cggtcagatt tctggggcgc
     1681 aaaccatcac ctataacaat caggagcaca cgctacaaca agcatcagta tggttaaaaa
     1741 atacagacag agcaattcgt gaaactgttt acaaaaaaat tactgcgaga cgattggaag
     1801 ataaaaagca gttagacgac ctgtacacta atttaattca gcttaggcat caggtggcac
     1861 tgaatgcagg tttcaaaaat ttcagagatt actcctttgc ggcattagag agatttgatt
     1921 acagcgttga agattgctac cgttttcatg aaagcataga attggaaata aagcctgtta
     1981 tcaatcagct tgaagaatac agaaaaacca aacttgatgt tgcgcactta atgccttggg
     2041 acttggcggt tgaccctaca ggaaaaccac ccataaagcc tttcagcaat acacaggaga
     2101 tggttgagaa aaccatttta tgtttaaaag aaattgatcc ggagtttgcg aattgcatca
     2161 cactgcttga tgagatgaat cgttttgatt tggattcgcg caaaggcaaa tcaccgggtg
     2221 gatacaacta tcctttatac gaaacagggg taccatttat tttcatgaac agcacaggac
     2281 agttgcgcga tttggtgaca atggtacatg aaagcggtca tgctgtacat gcagtgctgg
     2341 cacacaatct tccttttcat gaaatgcgaa gctgcccatc agaagtggca gaacttgctt
     2401 ctatgtcaat ggaactgctg acaatggagc actggcatca ttttatttca gatgaaaaag
     2461 aattaaaacg tgccaaactt gaacacctaa ccagcttaat tgaaattctg ccatggatag
     2521 caattgttga tgcttttcaa cagtgggtat atctgcatcc tgaacatagt gtaactgaac
     2581 gcgaacagca atttgtccaa ctatacaatc gctttagtgg cactgtcgtt gattggcaag
     2641 gttttgaaaa tgtaaaagaa aatttatggc agaaacagat tcacattttt gattctcctt
     2701 tctattatat cgaatacgga atggcgcaat taggtgctat agcattatgg aagaattata
     2761 aaactgatgc cactgctact ataagaaatt acaaaaaagc attggctcta ggttatacaa
     2821 aatcaattgg tgaaatttat aaagccgcag gggtgagctt tgatttttct gcaacttaca
     2881 ttaaagagct ttcgcagttt gttcagtcag agataaagga actacaaaaa taattgcaac
     2941 attccctttc ttatcttcgt cagccctttt agcaagggtt gttgcccctc ttatatttaa
     3001 aaattaaaaa caaacatttg ctttaacata gtatgcaggt aaaatccaca tacctaaaca
     3061 tctggcaaat tgcctatccc attatgctgg catcggtagc acaaatgctt gtcgggctaa
     3121 cagacacagc attccttgcg cgtgttggcg aagtggagct aggagcatct gctattgccg
     3181 gagtgtttta ttttgtttta gtcatgttgg gcatggcttt gtctattgg
//
LOCUS       DAOQNP010000147         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_15910_length_3129_cov_6.870527, whole genome shotgun sequence.
ACCESSION   DAOQNP010000147 DAOQNP010000000
VERSION     DAOQNP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15910_length_3129_cov_6.870527"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..2347)
                     /locus_tag="PKY09_12410"
     CDS             complement(293..2347)
                     /locus_tag="PKY09_12410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNB13696.1"
                     /translation="MKQIFILACCAVSLAAIFAFKKKEPQQTVALTAAKAPKAIDLSN
                     FDKSVNPGDDFYQYVNGNWLKKNPLPASETRWGSFNVLDDITRHRVLTILEETAKNKN
                     AVEGSSAQKIRDFFLSAMDTALIEESGFLPIVPIFEKVDKVTDKNQLPALVAYLHTIG
                     INSFFNFYVGQDDKKSDQIIITLYQGGLGLPDRDYYLKDDKESVEIRTKYLSHIKNMF
                     LLMSIQPEGNAVTNLMSLETELARISRSRVDLRDPERNYNKMVTEDFNKEMSAFSMEK
                     YFGSFGVKENEIIVGQPEFFKRFNELMEKLPLEDLKFYLKWDILNSRASTLSSDFVKT
                     DFEFYQGVLSGAKEMRPRWKRVLGTMNGTMGELIGELYVKKYFSPEAKDRVNKMVDNL
                     ITAYQQRIKSRTWLEDQTKVAALAKLEKVIRKLAYPDKWRDYSKMIIKKDSYCNNVNR
                     AAEYWFQYNLSKLGKPVDKTEWGMSPQTINAYYNPGFNEIVFPAAIMQPPFFDPLADD
                     AVNYGSMGAVIGHELTHGFDDQGCQYDADGNLKNWWTDKDKTLFNQNAQKLVAQFNSY
                     VVIDSLTINGQLTLGENIADLGGLTIAYAAFQKTENAKSKTLLDGFTPQQRFFINFAQ
                     VWRNNIRPEELKKRLKTDPHSPGKFRANGTVRNMPEFYEAFKVTKENKLYLAPEQRVE
                     IW"
     gene            complement(2416..>3129)
                     /locus_tag="PKY09_12415"
     CDS             complement(2416..>3129)
                     /locus_tag="PKY09_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNB13697.1"
                     /translation="VANPATILCQMIASLHDENNHITVKGFYDDVLTVSDADRGDLAK
                     IPFDEKEYMKDLGINEVWGEKGYTSIERTSIRPTLEVNGIWGGYTGEGAKTVLPSKAF
                     AKISMRLVPNQSSDNISKLFEKHFLSIAPPFVKVKVSAHHGGEPVLTPTDSVPFKAAS
                     MAMEKTFGKKPIATRGGGSIPIVALFEKELGLKTVLFGFGLDSDNLHSPNEKYGLFNF
                     YKGIETIPYFFQYFTALKK"
BASE COUNT          878 a          634 c          577 g         1040 t
ORIGIN      
        1 gtgtgctggc agttttggca ggtgttgcct ttacttcttt tttagttgtg gtagcggcct
       61 gcttggtggt gcctgactgt gcctgagaaa aagtaatgcc taatcccagt gcaataatta
      121 aaagtaactt tttcattttg tataaatttt gatggggtca aatgtgtagt tggcaacatg
      181 atactactat gaaaacacaa aaccctttta tgtggaattg ctgaagaaaa ataggtacat
      241 aaaaaaactg ccccgaaatt atcggggcag ctgcttcatt taatactatt tcttaccata
      301 tttccactct ctgttccgga gccaaataca acttgttttc tttagttact ttaaatgctt
      361 cataaaactc cggcatattg cgtactgtac cgttggcacg gaatttaccc ggactgtgtg
      421 gatcggtttt caatcgtttt ttcaactctt cggggcggat gttgttgcgc caaacctgtg
      481 caaagttgat gaagaaacgt tgctgtggtg taaagccgtc caacaaagtt tttgacttgg
      541 cattttctgt tttttggaag gcagcatagg ctatagtcag tccgcctaaa tcagcaatgt
      601 tctcacctaa agtaagctgt ccgttgatgg tgagtgaatc aattactacg taactattaa
      661 attgtgctac cagtttttga gcattttgat taaagagtgt tttgtcttta tctgtccacc
      721 agtttttcag attaccatcg gcatcatact ggcaaccctg atcatcaaaa ccgtgtgtga
      781 gctcatgacc aatcactgca cccatagagc catagttaac agcatcgtca gccagaggat
      841 caaagaatgg cggctgcatg atagctgcag gaaaaacaat ttcattgaag cccggattgt
      901 aataggcatt gattgtttgt ggcgacatgc cccactctgt tttgtcaacc ggctttccta
      961 attttgaaag gttgtactga aaccaatatt ctgccgcacg atttacattg ttacaatagg
     1021 aatctttttt aataatcatc ttagaataat cgcgccattt gtcaggataa gccagctttc
     1081 ggataacctt ttctaatttt gccaatgctg caacttttgt ctgatcttcg agccaggtgc
     1141 gtgatttgat gcgttgctga taggctgtaa taagattatc taccattttg ttgacacgat
     1201 cttttgcctc cggactaaaa tactttttca catacaactc tcctatcaac tcacccatag
     1261 taccattcat ggttcctaat acacgcttcc agcgtggacg catttcttta gcaccactca
     1321 atacgccctg ataaaattca aaatctgttt taacaaagtc tgagcttaat gtacttgcac
     1381 ggctgttaag gatatcccat ttaagataaa attttaaatc ttccaatggt agtttttcca
     1441 ttaactcatt gaaccgttta aaaaattctg gttgtccaac aatgatttca ttttctttta
     1501 caccaaacga gccaaagtat ttctccatag aaaatgcaga catttcttta ttaaaatctt
     1561 cggtaaccat cttattgtaa tttctttcgg gatcgcgcaa atcaacacga gagcgtgata
     1621 ttcttgccaa ctcagtttct aaactcatca ggttggtaac ggcatttcct tccggctgaa
     1681 tgctcatcaa cagaaacata tttttaatat gcgataaata tttcgttctg atttctactg
     1741 actctttatc atctttcaga taataatctc tgtcgggtaa tccaagccca ccctgataga
     1801 gtgtgataat tatctgatca cttttcttat catcttgtcc tacataaaaa ttaaaaaaac
     1861 tgtttatacc aatggtatgc agataagcta ccagtgcagg cagttgattt ttatctgtta
     1921 ccttatcaac tttttcgaaa atcggaacaa tgggtaagaa gcctgattct tctattagtg
     1981 cagtatccat agcagacaaa aagaaatcac gtatcttctg tgcagagcta ccttcgacag
     2041 cgtttttgtt ttttgctgtc tcttcaagaa tggtaagcac tctgtgacgt gtaatatcat
     2101 ccagcacatt gaaagagccc catcgggttt ctgaggcagg aagtgggttc ttttttagcc
     2161 agttgccgtt tacatattgg taaaaatcat ctccgggatt tacactcttg tcgaagttgg
     2221 ataaatcaat agcctttggc gctttagcgg ctgttagtgc aacagtttgc tgtggttctt
     2281 ttttcttaaa tgcaaaaatt gctgcaagag aaactgcaca gcaggcaaga ataaatattt
     2341 gtttcattag tataataaat taagtaacgg attttaaaaa tttaactttg tttgaaaagg
     2401 taattttctt tggtgttatt ttttcaacgc tgtgaaatat tgaaagaagt aaggtattgt
     2461 ttctatgcct ttatagaagt taaacaaacc atacttttca ttgggagagt gcaggttatc
     2521 gctgtctaac ccaaagccaa acaacaccgt tttcagacct aactcctttt caaacagtgc
     2581 aacaatcgga atacttccgc cacctctggt ggctatcggt ttcttaccaa aagttttttc
     2641 cattgccata ctggctgctt taaatggaac actatctgta ggagttaaaa caggttcgcc
     2701 accgtgatgg gcagaaactt ttactttgac aaaaggtggt gcaatggata gaaaatgctt
     2761 ttcgaaaagc ttggaaatat tgtctgagct ttgattcggt accagacgca tggagatttt
     2821 tgcaaatgct tttgagggta atacagtttt agcaccttca ccggtgtagc ctccccaaat
     2881 accatttacc tcaagtgttg gtctgatact ggttcgctca atggaagtat atcctttctc
     2941 gccccatacc tcattaatgc ccaaatcttt catatattct ttttcgtcaa aaggtatttt
     3001 agccaaatct cctctgtcag catcactcac cgtcaacaca tcatcgtaaa acccttttac
     3061 agtgatatga ttattttcat catgtaacga tgcaatcatc tgacaaagta ttgttgcagg
     3121 atttgcaac
//
LOCUS       DAOQNP010000148         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_17437_length_2984_cov_5.523728, whole genome shotgun sequence.
ACCESSION   DAOQNP010000148 DAOQNP010000000
VERSION     DAOQNP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17437_length_2984_cov_5.523728"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKY09_12420"
     CDS             <1..923
                     /locus_tag="PKY09_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNB13698.1"
                     /translation="PFSLNDESYVMAFIIDITARKKVQAQMLEQKNQLEDITRMLKQS
                     NERLEAKVLDRTKVLEEALNEIEKSRKELSEALAKEKELNELKSRFLSMASHEFRTPL
                     TTILSSASLITAYIEKEQQDKRIRHVERIKSAVNNLNDILSDFLSLSKIEEGKTKTDY
                     EVFNLNNIISEIINDLATIKKPGQAINYVHTGKNDVLLDPRLIRNIVINILSNAIKFS
                     DEGKLIEIKTDIGENTTVLEFKDQGIGISKEDQQHLFERFFRGLNATNIQGTGLGLNI
                     VARYVELMNGVIETESELNKGTAFKITIPC"
     gene            926..1813
                     /locus_tag="PKY09_12425"
     CDS             926..1813
                     /locus_tag="PKY09_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNB13699.1"
                     /translation="MNISNPIIFNGHGSAVYCLLSKNQNAFYSGSGDHHLIEWSIETP
                     DKGTVVAILPAGIYSFTLFNDLLFAGCGNGEIHSVNIKNTSALAKAHLHNSYVFDLFL
                     NNHQLYTAGGDGQIIRTGLQLNKHASVRLGDFKIRKLLPHPDEQHIIAGCGDGTVRII
                     NSNTLKEEQIITAHNTGFSVNALCFTPDKKFLLTGSRDARLNVFDVQQNFKLIESIAA
                     HNYAIYAIAFNPSGKLMATASRDKKIKIWNDDLSFISRIEEHPVGHKNSVNTLCWLSD
                     TELITTGDDRSIMLWHLNA"
     gene            complement(1821..2246)
                     /locus_tag="PKY09_12430"
     CDS             complement(1821..2246)
                     /locus_tag="PKY09_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNB13700.1"
                     /translation="MQQGNINVVEAEIIADFELFDDWTQKYEYIIELGQQLPALEERF
                     KKEEYKIKGCQSSVWLNAFENDGLLFFEADSDSVFVKGEIALLIKVLSGRTPEEILSA
                     ELKFIDAIGLRQHIAVTRANGLASMIKQMKAYALAHKEG"
     gene            complement(2249..>2984)
                     /locus_tag="PKY09_12435"
     CDS             complement(2249..>2984)
                     /locus_tag="PKY09_12435"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281829.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNB13701.1"
                     /translation="SRRENVTDFNLLESCLREHLNKVALRTMAVINPLKVVITNYEEG
                     KTETLQAENNPEATEKTYRNVAFSRELFIEQDDFMEHAPEGFFRLTVGSMVRLKHAYI
                     IKCENVVKHDDGTIKEIHCTYVAESKSGQDQSGLKVKSVIHWVNATSAIDAEVRLYEK
                     LFTVEAPDAQEEDFKKFINPHSLQIFSNAKVEPDLLHHEKGERFQFLRLGYFVEDKYS
                     TSAKRIYNRAVTLKDEWAKEQKKQKK"
BASE COUNT          956 a          585 c          512 g          931 t
ORIGIN      
        1 gtcctttttc tttaaatgac gaatcgtatg taatggcatt tattattgac ataaccgcaa
       61 gaaaaaaagt tcaggcacag atgcttgaac agaaaaatca gctcgaagat attacccgta
      121 tgctaaaaca aagcaatgag cgtttggaag caaaagttct tgaccgtaca aaagttttag
      181 aagaagcact caacgaaatt gaaaagtcca gaaaagaatt aagcgaagct cttgcaaaag
      241 aaaaggaatt gaatgaactc aaatcacggt tcttgtcaat ggcatcacat gagtttcgta
      301 caccactcac caccattctt tcatcagcat cattaattac cgcatatatt gaaaaggagc
      361 agcaagacaa gcgtatcaga catgttgaac gaataaaatc tgccgttaat aatctgaatg
      421 atattttaag tgacttcctt tcgttgagta aaattgaaga aggaaaaaca aaaaccgatt
      481 atgaagtatt caatctgaat aatataatca gtgaaattat taatgatctg gctacaataa
      541 aaaaacccgg acaagcaatt aattatgtac atacaggaaa aaatgatgta ttgctcgatc
      601 caaggttgat tcgaaatatc gtcatcaata ttctttcaaa tgccataaaa ttttctgatg
      661 aaggaaagct cattgaaata aaaacagata ttggtgagaa taccactgtg cttgaattta
      721 aagatcaggg aattggaatc agtaaggaag atcaacaaca tttgtttgaa cgctttttca
      781 gaggattgaa tgctaccaac atacaaggca caggacttgg cttaaatatt gttgcccgct
      841 atgttgagct gatgaacggt gtaattgaaa ccgaaagtga actaaataaa ggaactgctt
      901 ttaagattac tataccctgt taataatgaa tatttctaat ccaataattt tcaatgggca
      961 tggcagtgct gtatattgcc tgttatcaaa aaatcaaaat gcattttatt ccggaagtgg
     1021 cgaccatcat ctgattgaat ggagtattga aacacctgat aaaggcactg ttgttgcaat
     1081 tcttccggca ggtatttata gttttacttt atttaacgat ttgctctttg caggctgtgg
     1141 caatggcgaa atacatagtg ttaatattaa aaatacatct gctttagcaa aggcacattt
     1201 acacaacagt tatgtctttg atttatttct taataatcat caactctaca cagccggtgg
     1261 tgatggacaa ataattcgaa caggcttaca attaaacaaa catgcatccg ttcgcttagg
     1321 tgactttaaa atcagaaaac tattaccaca tccggacgaa caacacataa ttgcaggttg
     1381 tggtgatggc actgtcagaa ttattaactc aaacaccttg aaagaagaac agattattac
     1441 tgcacacaac acaggttttt ctgtcaatgc actttgcttt acgcctgata aaaaatttct
     1501 tctgacaggc tctcgtgatg cacgacttaa tgtattcgat gtacaacaaa attttaagct
     1561 aattgaaagt attgccgctc acaattatgc aatttatgct attgctttta acccctccgg
     1621 aaaattgatg gcaacagcca gtcgtgataa aaaaataaaa atctggaatg atgatttatc
     1681 ttttatttcc cgtattgaag aacatcctgt aggtcataaa aattctgtga acacactttg
     1741 ttggctctca gatacagagc taatcaccac gggtgatgac agaagtataa tgttatggca
     1801 tctcaatgca taataaataa ttacccctct ttatgcgcta gtgcataggc tttcatctgc
     1861 ttaatcatgc tggccaaacc attagcacgg gtaacagcaa tatgttggcg cagacctata
     1921 gcatcaataa atttcaactc tgcagatagg atctcttccg gtgtgcgtcc actcaatact
     1981 tttatcagta aagcaatttc gccctttaca aaaacagaat cgctgtcggc ttcaaaaaaa
     2041 agcaaaccat cgttttcaaa agcattgagc caaacactac tctggcagcc tttaatttta
     2101 tattcttctt tcttaaaacg ctcttctaat gccggcagtt gctgacccag ttctatgatg
     2161 tattcatact tctgtgtcca gtcgtcaaac aactcaaagt ctgcaataat ttctgcctcc
     2221 actacattta tatttccctg ctgcatggct attttttttg tttcttctgt tctttcgccc
     2281 actcatcttt taaagtcacc gcgcggttgt atattctttt tgcagaggtg ctgtacttgt
     2341 cttctacaaa atagcccaat cttaaaaact gaaaacgctc tcctttttca tgatgtaaaa
     2401 ggtcgggttc tactttggcg ttgctgaaaa tttgtaatga atgtggattg ataaactttt
     2461 taaaatcttc ttcttgtgca tcaggtgctt caacagtaaa cagcttctcg tataaccgta
     2521 cttctgcatc aatagcactg gttgcattca cccaatgtat cacacttttt actttcaagc
     2581 ctgattgatc ctgtccactt ttactctcag caacataggt acaatgaatc tctttgatgg
     2641 taccatcatc atgtttcacc acattttcgc acttgataat gtaggcatgt tttagccgca
     2701 ccatactacc cacagttagc ctgaaaaatc cttcaggggc atgctccata aaatcgtcct
     2761 gctcaataaa caattcacga ctgaaggcaa cgttgcggta tgttttttca gttgcttcgg
     2821 gattgttttc tgcttgtaag gtctctgttt ttccttcctc ataattagta attacaactt
     2881 tgagtggatt gataacggcc attgttctta gtgcaacctt attcaagtgt tcacgaagac
     2941 aagattccag caaattaaaa tctgtaacat tctctcgtct tgat
//
LOCUS       DAOQNP010000149         2973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_17566_length_2973_cov_7.722070, whole genome shotgun sequence.
ACCESSION   DAOQNP010000149 DAOQNP010000000
VERSION     DAOQNP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17566_length_2973_cov_7.722070"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKY09_12440"
     CDS             complement(<1..691)
                     /locus_tag="PKY09_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13702.1"
                     /translation="MPRACMEFNDVVMTIGDYQYNEFRNSNWGIYAVRSMLKVQKGRF
                     YNMNKGGSLYGSAAHKGSAIVAESKTAAKAGKLQVADCLIDTCEHGIYTEWTTADINN
                     VNALHVTGNGSYHLYCNSVNMSTYVGACNIVAGKSGIVFQNSEQATMTAKNNTIKVTT
                     GGSSVGIKIIATNSNAGNYVLKGNVIEAANGNGITVNFAKNATIFDNMIKLSGNTING
                     IALTGCDSSTVS"
     gene            complement(699..2213)
                     /locus_tag="PKY09_12445"
     CDS             complement(699..2213)
                     /locus_tag="PKY09_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13703.1"
                     /translation="MKIIKNNSIFKMLLFLILIVVLKTTAFSQGRNSIFFLGDQAKAR
                     ISFTGTSYSIQTETRTIPFMDTQGNIADENGNILMSSNGVFIADAIGDTMVNGDSLNP
                     NSFTDDFKQYGLPMPYANLFLPMPDDSNKYVLFHQVLDYTTANVPNIFYTTVDITLNG
                     GLGAVISKNNIIATNAFGVGMAACKHANGRDWWVVALSFDATLAHTFLVTPNAVQYMG
                     SQNLQLAGFPLGFGGQPTFSPDGEKFAFSCSNIVGMSYQSKVQLFNFDRCSGVFSLDT
                     VIDFTDGNISFATAFSPNSKYLYFGTTENLYQINTDTSDIGATFQLVATNDIFPSSVP
                     PFYTNFYLMYLAANGKIYISSTSSVEHLHEINYPDSAGVACGVQLHNVFTDCYFIGVP
                     NHPNYYLGCDTTQTTCPCLITGINEIKQHDFKFSVSPNPSNGNFKIMYLLPQNSKGIF
                     EVFDITGKKVFSYSLPQWSTLQNFDLSFLSNGVYAVKITSGNFSITKKLIVYKE"
     gene            complement(2210..>2973)
                     /locus_tag="PKY09_12450"
     CDS             complement(2210..>2973)
                     /locus_tag="PKY09_12450"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13704.1"
                     /translation="YTYDSTLMALFDSCNLQITTLTDSIEKLDENHPAGWEVIRDQVL
                     YTIEFLNQKIYNLHLQREALFNDNLTNAELQNSYVVNGELPELNSAYMNEMEINYMET
                     DEDLQFLRDNYSGIFAIASQCPYVGGKAVERARTYIALINDSISYDDANICLQSGVYR
                     FANDTTENIKTTEIKIIPNPANDKVTIQLKGIDDGICKIQIRNVLNEIVYDDVFDCKN
                     QKHIINVSNLSQGVYSITINAIGKKSIINKLFITR"
BASE COUNT          891 a          569 c          505 g         1008 t
ORIGIN      
        1 aacttaccgt gctgctatcg caacctgtga gggcaattcc gttgatggtg ttgccactta
       61 gctttatcat gttgtcaaag atggtagcgt ttttggcaaa gttaaccgtt atgccattgc
      121 cgttggctgc ttcaatcaca ttgcctttta acacatagtt gcctgcattg ctgttggtag
      181 ctattatttt tattcccaca gagctgcctc ctgtggttac ttttattgtg ttgttcttag
      241 ccgtcatggt tgcctgctcg ctgttttgaa atacaatgcc tgacttgccc gctactatat
      301 tgcaagctcc aacataggtg ctcatgttga cactgttgca gtacaaatgg tagctgccgt
      361 tgccggtaac gtgcagggca tttacgttgt tgatgtctgc tgttgtccac tctgtgtaaa
      421 tgccatgctc gcaggtatct atcaggcagt cggcaacctg caattttcct gccttggcag
      481 ccgtcttgct ctcggcaaca atggccgagc ctttatgtgc agcactgccg taaaggctgc
      541 cgcctttgtt catgttgtaa aaacgtcctt tctgaacttt cagcatagaa cgcactgcgt
      601 aaatgcccca gttgctgtta cgaaactcat tgtactggta gtcgccaata gtcattacca
      661 catcattaaa ctccatgcag gcacgaggca tcgcattttt attccttata tactattaat
      721 ttttttgtaa tactaaaatt accggatgtt attttaactg cgtaaacccc attactcaaa
      781 aaactcaaat caaagttttg taaggttgac cattgaggca atgaataact aaaaactttt
      841 ttgcctgtaa tatcaaatac ctcaaaaatt cccttgctat tctgcggcaa caaatacatt
      901 attttaaagt tgccgtttga tggattgggt gaaacagaaa atttaaaatc atgctgctta
      961 atttcattta tgccagttat caaacatggg caagttgttt gcgtagtatc acaccctaaa
     1021 taataattgg gatgattggg cacaccaata aaataacaat ccgtaaaaac attatgtaac
     1081 tgaacaccac aagctacgcc tgcactgtca gggtaattta tttcatgcaa atgctctaca
     1141 ctacttgttg atgaaatgta aatcttgccg tttgcagcaa gatacattaa ataaaaatta
     1201 gtataaaaag gcggaacaga cgaaggaaaa atatcattgg ttgcaactaa ctgaaaagtt
     1261 gcaccaatat ccgaagtatc agtattaatt tgatataaat tttctgttgt tccaaagtag
     1321 aggtattttg aatttggtga aaatgcagta gcaaatgata tatttccatc agtaaagtca
     1381 ataacggtgt ctaagctaaa aacccctgaa cacctgtcaa aattgaacaa ctgaactttt
     1441 gattggtaac tcattccaac aatattgcta catgaaaaag caaatttttc accgtcaggt
     1501 gaaaaggttg gttgtccacc aaaccctaat ggaaaacctg ctaattgcag gttttgactt
     1561 cccatatatt gaacggcatt tggtgttact aaaaaagtat gagcaagagt tgcgtcaaaa
     1621 gataatgcca ctacccacca gtctctacca ttggcatgtt tacaggcagc cattcctaca
     1681 ccaaaagcgt tagtagcaat aatattattc ttgcttataa cagcacctaa tcccccattt
     1741 aatgttatat caaccgttgt ataaaaaata ttaggtacgt tagcagttgt gtaatctaaa
     1801 acctgatgaa acaaaacata tttatttgaa tcatcaggca ttggcaaaaa taaattagca
     1861 tagggcattg gcaagccgta ttgcttaaaa tcatctgtaa aggaattggg atttaagcta
     1921 tcaccattca ccatggtgtc tcctatggca tcagctatga aaactccatt gctgctcatt
     1981 aaaatatttc cgttttcatc agcaatattt ccctgtgtgt ccataaaagg tattgttctt
     2041 gtttccgttt ggatactata agaagtgcca gtaaaactta ttcttgcttt tgcttgatcc
     2101 cccaagaaaa aaattgagtt tctgccttgc gaaaatgctg ttgtttttaa tactactatt
     2161 agtattaaaa acaacagcat tttaaaaatg ctgttgtttt taataatttt catcgtgtaa
     2221 taaataattt attaatgatt gattttttac caatggcatt tatggtaata gaataaaccc
     2281 cctgacttaa atttgaaaca ttgattatgt gtttttgatt tttgcaatca aatacatcat
     2341 catatactat ttcatttaaa acatttctga tttgaatttt acatattccg tcatctattc
     2401 cttttagttg aattgttact ttatcatttg cagggtttgg aattatttta atttcagttg
     2461 tttttatatt ttctgttgta tcatttgcaa atcggtacac accactttga aggcaaatat
     2521 tagcatcatc ataagaaata gaatcattta ttaaagcaat gtatgtacgt gctctttcca
     2581 ctgccttgcc tcccacatag ggacattggc ttgcaattgc aaatattcct gaatagttat
     2641 ctctgaggaa ttgtaaatcc tcatctgttt ccatatagtt tatctccatc tcattcatgt
     2701 aagcactatt caactcaggt aattcaccgt tgaccacata actgttttgc agttctgcat
     2761 ttgtaagatt atcattaaat aaggcctctc tctgtaaatg taaattatat attttttgat
     2821 tcagaaattc tatggtgtaa agaacttgat ctcttatgac ttcccatccg gcagggtggt
     2881 tttcatccaa cttttcaatg ctatcggtta atgtggttat ttgcaaatta caactgtcaa
     2941 ataatgccat taaggtagaa tcataagtgt atg
//
LOCUS       DAOQNP010000150         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_17947_length_2940_cov_5.516118, whole genome shotgun sequence.
ACCESSION   DAOQNP010000150 DAOQNP010000000
VERSION     DAOQNP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17947_length_2940_cov_5.516118"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKY09_12455"
     CDS             complement(<1..566)
                     /locus_tag="PKY09_12455"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNB13705.1"
                     /translation="MSLKRAYIFPGQGSQFSGMGKDLFEQSDAAKRIFEKANSITGFA
                     ITEIMFGGTDEELKQTKVTQPAIFIHSCAVIASLGENFHPDMVAGHSLGEFSALVANR
                     CLSFEDGLKLVMERALAMQAACELKPSTMAAILGLEDEKVETICAGVNDVVVCANYNC
                     PGQLVISGTIEGVKAACEQLTAAGARRA"
     gene            complement(654..1064)
                     /locus_tag="PKY09_12460"
     CDS             complement(654..1064)
                     /locus_tag="PKY09_12460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNB13706.1"
                     /translation="MIEFEHQVRVRYAEADRMGYVYYGNYAVYFEVARVEMLRSLGLP
                     YKSLEDKGIILPVSDFSVQYKKPAFYDDLLTIKTTIRKKPSVRIVFDYETFNEKGELL
                     NTASVTLVFVSKATGKPVNPTDEFNIVFTPYFQN"
     gene            1188..>2940
                     /locus_tag="PKY09_12465"
     CDS             1188..>2940
                     /locus_tag="PKY09_12465"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNB13707.1"
                     /translation="MNSFLYHLKSKQLQSYNLKLLSLLLGIFSFNTGLSAMTSIADTF
                     HIEKYIISIDTIDFTGKQIKAKTQIQIVVKQNNLSAIEMDLFQLTVDSVYYPLGNLPY
                     SYQNDKLNISLPVVLNTNDTISLTVSYQGSPKKDASGFGGFYFFPNMAFNLGVGFTAD
                     PHNLGKVWFPCLDVFDDKSKYEFYITTPLTQKAYCNGFLNDSLINANNTITWHWIMND
                     YIPTYLACMATGPYTSWSRTYNGIPVEIAALPADTAAVSATFVNLPAAINLFTQYYGS
                     YPFNKIGYTLVNFTGGAMEHATNIAIGKAFINGTLGYETLWAHELSHMWWGDKVTCKT
                     AEDMWLNEGWASFNEALFTEAIYGPTAYKDWIRTNHRKVLQLAHITDGGYLALNNVPT
                     SVTYGNTAYQKGADIVHTLRNYAGDSLFKVGAQHYLDTFAYSNASSIDMEHAFTSSTG
                     TDYSRFFTDWVYTPGFPHFSIDSAIYVPGGLDHYFVYTRQRNKGSNGHIYSMPVDITF
                     SDGITDTTVSIQIDSATQMFHIPLLMANATWIALDRGEKISDAISDYEKIITTTGLQI
                     MKETNSTLNVQSVGATPS"
BASE COUNT          937 a          573 c          511 g          919 t
ORIGIN      
        1 aatgcacgtc ttgcaccggc agcagtgagt tgttcgcatg cagcttttac accttcaata
       61 gttccggaaa taaccaattg accgggacag ttataattgg cacaaacaac aacgtcatta
      121 actccagcac atattgtctc cactttttcg tcttccagac caagaatagc tgccatagtt
      181 gaaggcttta attcacatgc agcttgcatg gcgagtgcac gttccatcac cagtttcagc
      241 ccatcttcaa aactcagaca tctattagct accaatgctg aaaattcgcc taatgaatga
      301 cctgcaacca tgtcgggatg aaaattttct ccgagcgaag caatgactgc acaggaatga
      361 ataaaaattg ccggttgtgt aaccttagtt tgtttcagct cttcatcggt accaccaaac
      421 ataatctctg taatagcaaa tcccgtaatg ctgtttgctt tctcaaaaat tcttttggcg
      481 gcatcagact gttcaaacaa atcttttccc attccggaaa attgtgatcc ctgaccgggg
      541 aaaatatacg ctcgttttaa actcataaaa tatttagtca gttatttcag ttgattttga
      601 ttaatgtgaa aatcttttgc caaagctaag taaaacaaaa taaaacaggt tgtctaattc
      661 tgaaaatatg gtgtaaacac tatgttaaat tcatcagtcg ggttaaccgg cttacctgtt
      721 gccttactca caaagacaag tgttaccgat gcagtattga gtaactctcc cttttcatta
      781 aaggtttcat agtcaaaaac aattcgtaca gaaggctttt ttctgatggt agtttttatc
      841 gtcagtaagt catcataaaa tgccggtttc ttatattgaa cagaaaaatc agataccggc
      901 aaaataattc ccttatcttc aagtgacttg taaggtaaac caagactgcg cagcatttct
      961 acccttgcca cttcaaaata aacagcatag tttccataat acacatatcc cattctgtcg
     1021 gcttcggcat atctaactct aacctgatgt tcaaactcaa tcattatatt tcgataatat
     1081 taaatgcaaa taaaaagaaa atgatgctat gaaaatttaa gtcagtattt ctagattaaa
     1141 aatttctata tttgaaagtt caattatgtc agcgtaattt tttatttatg aattcatttt
     1201 tatatcatct aaaatcaaaa caacttcaat catataattt aaaactttta agtctgttgc
     1261 tgggtatatt ttcctttaat accggcttgt cggctatgac ttcaatagca gatacttttc
     1321 atattgaaaa atacattatc agtattgata ctattgactt taccggtaaa cagattaaag
     1381 caaaaacaca aatacaaatt gttgtcaaac aaaataatct aagtgccatt gaaatggatt
     1441 tgtttcagtt gactgttgat tctgtttatt atccattggg aaacctaccc tattcgtatc
     1501 aaaacgataa actaaatatt agtcttccag ttgtattaaa cacgaatgac acgataagtt
     1561 taaccgtatc ctatcaaggt agtcctaaaa aagatgcatc aggctttggc ggcttttatt
     1621 tttttccgaa catggctttt aatctcggtg taggtttcac tgccgacccg cacaacttag
     1681 gtaaagtttg gtttccttgt cttgatgttt ttgatgataa aagtaagtat gaattttata
     1741 ttacaacacc actgacacaa aaagcctatt gcaatggttt tttaaatgat tcactcatta
     1801 atgcaaacaa caccatcacc tggcattgga taatgaatga ttatattcca acttatctgg
     1861 cttgcatggc taccggacca tacacctcat ggtcgagaac ttacaatggt attcctgttg
     1921 aaattgcagc attgcctgcc gatactgcgg cagtgtcagc cacgtttgtt aacctgcctg
     1981 cagcaataaa tttgtttaca cagtattatg ggagttatcc tttcaataaa ataggatata
     2041 cattggtgaa ttttaccggt ggtgccatgg aacatgccac taacattgcc attggcaagg
     2101 cttttattaa tggcacactt ggttatgaaa cattgtgggc acatgagtta tcgcacatgt
     2161 ggtggggcga taaggtgacc tgtaaaactg ctgaagacat gtggctaaac gaaggctggg
     2221 catctttcaa tgaagcgttg tttacagaag caatttatgg accaacagcg tataaagact
     2281 ggattaggac aaatcacaga aaagtattgc aactggcaca tattaccgat ggtggatatc
     2341 ttgcattgaa taacgttccg acctcggtta cctatggaaa tacagcatat caaaaaggtg
     2401 ccgatattgt tcatacttta agaaattatg caggcgattc attatttaaa gttggagcgc
     2461 aacattattt agacactttt gcttatagca atgcatcgag tattgacatg gaacatgcat
     2521 ttacttcttc cacaggaaca gactattcac gtttttttac agattgggtt tacactccgg
     2581 gatttccaca tttttctatt gactctgcaa tatatgttcc cggaggattg gatcattact
     2641 ttgtttacac ccgtcagcgt aataaaggca gcaatggaca catttattcc atgcccgtag
     2701 atattacctt tagtgatggc attactgaca ctactgtgag catacaaatt gactctgcca
     2761 cacaaatgtt tcatattcca ttgctgatgg caaatgcaac atggatagca ttagacagag
     2821 gtgaaaaaat cagcgatgca atatcggatt acgagaaaat aattacaact acgggtttac
     2881 agattatgaa agaaacaaat tcaacattaa acgtacaatc agttggcgca accccatcaa
//
LOCUS       DAOQNP010000151         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_19104_length_2844_cov_10.089996, whole genome shotgun
            sequence.
ACCESSION   DAOQNP010000151 DAOQNP010000000
VERSION     DAOQNP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19104_length_2844_cov_10.089996"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKY09_12470"
     CDS             <1..567
                     /locus_tag="PKY09_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNB13708.1"
                     /translation="IQSAYNLVNPMVPLWAVGIVARDSIIIEFSPTIIMLVLAGKVGS
                     SVASEIGTMRVTEQIDALEIMGINSSGYLVLPKIIAALLICPVVVIISMIVGITGGWV
                     AGDLSGVVPSADYLKGVQNQFLPFNVTFAMIKTIVFAFIITSVPAYHGYYTSGGALEV
                     GQSSTKGVVYSSILILIFDYLLTQLILA"
     gene            564..1316
                     /locus_tag="PKY09_12475"
     CDS             564..1316
                     /locus_tag="PKY09_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNB13709.1"
                     /translation="MIAIENISKSFGNKKVLEDVSCEFDKGKINLIIGGSGQGKTVLM
                     KCAVGLFEVDKGKVLYDGRNFSEMTFGERKPIRQEIGMLFQGGALFDSLTVEQNVTFP
                     LKMFTNMTSAEKRDRANFCLHRVNLENVNHLLPSAISGGMKKRVAIARAIAMNPKYLF
                     CDEPNSGLDPKTSILIDELIKEITEEYDITTVINTHDMNTVMSMGEKIIFIYKGKKFW
                     EGNNEDIMRSENKELNDFVFASRWMQAVKKHF"
     gene            complement(1328..2626)
                     /locus_tag="PKY09_12480"
     CDS             complement(1328..2626)
                     /locus_tag="PKY09_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13710.1"
                     /translation="MRKLYISILFIISAGITQAQIDNLSNMSPEWIRTGVRNAAIKGC
                     DLVVYSPATLTRLEKGLHIGFGNQIFMRKPSHSYDLGWGEIKREQDGNDYLVPNIYLS
                     YNKNKWAVWGGVYISGGGATANYPEGSLNTDLISLMLLDAFQGAYTTTGKEYLKASSM
                     YLTGTVGFSYAINEQFSAALGMRYINATNQTKAGTELIGDFGSLPVNMEQKDNASGIA
                     AVLSLNARPVEKLNIAVRIETNAKLNFKTKQKVDDLEQSSGGMAELVTDGAENRRDLP
                     GLAGIGIGYEFSKAFRLTAEMNYYFQQHADWGKRADGKRINTLAGDAMNFGGGFEWMW
                     SKYITWSAGAVYTMLDFNDMAGYYTTLGAFETAPYNNLTANTGFAIDLSEKFRVNVGI
                     AKAFYKKDKNVNIEAMYPYMVQAKLNNDVLMLGAGLDLRF"
BASE COUNT          828 a          563 c          553 g          900 t
ORIGIN      
        1 attcaaagtg cttacaacct tgtgaaccca atggtgcctt tgtgggctgt tggaattgtt
       61 gctcgtgata gtatcatcat cgaattttct ccaaccatca tcatgcttgt gcttgcagga
      121 aaagtaggta gcagtgttgc atcagaaatt ggtaccatgc gtgtaaccga acagatagat
      181 gcacttgaga taatgggtat aaactcatca ggatatttgg tgttacccaa aattattgca
      241 gcactattaa tttgtcccgt tgttgtaatc atcagtatga ttgttggcat tacaggtgga
      301 tgggttgccg gtgatctttc cggtgtagta ccttcggcag attatttaaa aggagtacaa
      361 aaccagttct taccattcaa tgtaactttt gctatgataa agacaatagt atttgcattt
      421 atcattactt ctgttccggc ctatcatggt tattatacaa gtggtggcgc acttgaagtt
      481 ggccagtcaa gtacaaaagg tgtagtttac agcagtattt taattctgat atttgattac
      541 ttattaaccc aactcattct tgcatgattg ccatagaaaa catatccaaa tcattcggca
      601 ataaaaaagt gcttgaagat gtttcatgcg aatttgataa gggtaaaatc aacctgataa
      661 taggtggtag cggacaagga aaaacggtat tgatgaaatg tgctgttggt ttgtttgagg
      721 ttgataaagg aaaagtatta tacgatggca gaaatttttc ggagatgacc tttggcgaac
      781 gcaaacccat tcgtcaggaa attggcatgt tgtttcaggg tggtgcattg ttcgattcat
      841 taacggttga acaaaacgta accttcccgt tgaaaatgtt taccaacatg accagtgcag
      901 aaaagcgaga tcgtgccaac ttttgtctgc atagggtaaa cctcgaaaat gttaatcatc
      961 ttcttccaag tgctatcagt ggtggcatga aaaaacgtgt agccatagca cgagccattg
     1021 ctatgaatcc taaatattta ttttgtgatg aacccaactc agggctcgac cccaaaacct
     1081 ccattcttat tgacgaactc attaaagaaa ttacagagga atacgatatt actactgtca
     1141 tcaacacaca tgacatgaat acggtgatga gtatgggcga aaaaattatt tttatttaca
     1201 aaggcaaaaa attctgggaa ggcaataacg aagacatcat gcgctctgaa aataaagagt
     1261 tgaacgattt tgtttttgcc tcacgatgga tgcaggcagt taagaaacat ttttgatttt
     1321 accgatttca aaaccgtaaa tccaaacctg caccaagcat taaaacatcg ttgtttagtt
     1381 ttgcctgaac catgtacgga tacatcgcct caatgtttac atttttatct tttttgtaaa
     1441 acgctttggc tatacctaca ttcactctga atttttctga taagtcaatt gcaaaaccgg
     1501 tgtttgcagt caggttgttg taaggtgctg tttcaaaagc tcctaatgtt gtgtagtaac
     1561 ctgccatgtc attaaaatcg agcatggtat atactgcacc cgcactccag gtaatatact
     1621 ttgaccacat ccactcaaaa ccaccgccaa aattcatggc atcgcctgca agggtgttta
     1681 ttctttttcc atcggcacgt ttgccccagt cggcatgttg ctgaaagtag taattcattt
     1741 ctgctgtcag acgaaaagct ttactgaatt cgtagcctat accaatgcct gccaatccag
     1801 ggaggtcgcg tctgttttct gcaccatcgg taactaactc agccatacca ccggacgatt
     1861 gttccagatc atcaaccttt tgtttggttt taaaattcaa cttggcattg gtttctattc
     1921 ggacagcgat gttcaatttt tctaccgggc gtgcattcag gcttagcact gcggcaatgc
     1981 cacttgcgtt atctttctgc tccatgttta caggcaggct gccaaagtcg cctattaact
     2041 ccgttcctgc ctttgtttgg ttggtggcgt taatatagcg catacctaaa gcggcagaaa
     2101 actgttcgtt aatagcgtaa gagaatccaa cagttcctgt taggtacatt gatgatgctt
     2161 taagatattc tttacctgta gtggtgtaag cgccctgaaa agcatctaac aacattaagc
     2221 tgattaagtc ggtgttgaga gagccttcag gatagttggc cgtagcacct ccaccggaaa
     2281 tatatactcc accccaaaca gcccatttgt ttttattgta cgacaaataa atattcggaa
     2341 ccagatagtc gttgccatct tgctctcttt ttatttcgcc ccagcccaaa tcataactgt
     2401 gtgagggttt gcgcatgaaa atctgattgc caaaacctat atgcaaccct ttttccaaac
     2461 gagtcagcgt tgccggacta taaactacca gatcgcaacc tttgattgct gcatttctga
     2521 ctcctgtacg tatccactcc ggactcatat tgctcaggtt gtcaatctgt gcctgtgtta
     2581 ttcctgcact aattataaaa agaatgctga tgtataattt tctcattcgc tgtattttta
     2641 tttgagtaaa aatattttgc atttatatag tcatttacta tatatcagaa attaataaac
     2701 aattcttttt taataagtgt tggggtatga gatttagaaa tttttcaaag tccataggaa
     2761 tggatgtttc acatttattt gcactttcaa aaacaaacca tatatttaca atcgaatttt
     2821 aaaatcctca accaatgaaa aagt
//
LOCUS       DAOQNP010000152         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_22606_length_2603_cov_6.365385, whole genome shotgun sequence.
ACCESSION   DAOQNP010000152 DAOQNP010000000
VERSION     DAOQNP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22606_length_2603_cov_6.365385"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PKY09_12485"
     CDS             complement(<1..1401)
                     /locus_tag="PKY09_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13711.1"
                     /translation="MQATLNASNFNGYNISCYGMQDGTLTVNASSGTAPYSYTWSNGD
                     TLATVDSLAAGYYSVRVRDAAGGDTTLQITLTQPEQLAITEMTPYYYANDYNVSTYGA
                     CNGSVTTQISGGVLPLTYHWEPGQQNILAPSNLCGRENTLTVTDLNGCKASSSINLKE
                     PQRDDWTMSGNWGSNPTDNFIGTNDSIDLVFKTNGNERLRIAANGTTNFKNNINLEQS
                     LLFKNNAKIAFRPATTGIPDILSFGADPDVNLLSVNPNCNPTLNTPLINQFKGTIQLY
                     DFITNNNNPFSTLSILEMGFDGFNSIIEAQGNNSAPNSNRLLINYYCGKDVFVGKANS
                     GDLTANNNLLVNGKMGIGISQQDLNVISNYKLIVEGAVGVREMRIVNPAQSWPDYVFA
                     DNYKLKSLSDVEQFVESNKHLPGFAPAAIVASEGQNIGQLQVLQQQTIEEMYLHIIAL
                     EKRISKLEQENKKLKSQ"
     gene            1625..>2603
                     /gene="wecB"
                     /locus_tag="PKY09_12490"
     CDS             1625..>2603
                     /gene="wecB"
                     /locus_tag="PKY09_12490"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNB13712.1"
                     /translation="MIKLLTIIGARPQIIKAAAISRAIKNSFSTRIKEVIVHTGQHYD
                     EKMSAVFFSELQIPGEDYNLKTGSGSHAVQTAEIMRLFEPILLHEKPDAVLVYGDTNS
                     TLAGALATSKLHIPLVHVEAGMRSFNKLMPEEINRICCDHVSTFLFSPTQTGIKNLIR
                     EGFSAKTNPPYSINNPKIFACGDVMYDNSLYFSAEAKLKSKILESLSISSGQFILCTI
                     HRDSNTDVAENLQAIFKALNNISLSANQPIVIPLHPRTAKAIAANQEDVLWQEIKNNN
                     LIKIIEPVSFLDMIRLESECNLIITDSGGVQKESYFFKKPCVILRNETEW"
BASE COUNT          720 a          521 c          537 g          825 t
ORIGIN      
        1 ttgagatttg agttttttgt tctcttgttc taatttgctg atacgcttct ccaatgcaat
       61 tatatgaagg tacatctctt caatagtttg ctgttgcaat acctgcaact gtccaatgtt
      121 ttgcccttct gatgccacaa ttgcagccgg tgcaaatcct ggcaggtgtt tattgctttc
      181 aacaaattgt tctacatcag ataggctttt taatttgtaa ttgtctgcaa aaacataatc
      241 tggccaggat tgtgccggat taacaatgcg catttctcgt acaccaactg cgccctctac
      301 aatcagttta tagttgctga tgacatttaa atcctgctga ctgatgccaa ttcccatttt
      361 accgtttaca agcaggttgt tgtttgctgt taagtcgcca ctgttggctt tgccaacaaa
      421 aacatctttg ccacagtaat agtttatcaa taatctgttt gagttggggg ctgaattgtt
      481 tccctgcgcc tcaatgatac tgttgaaccc atcaaaaccc atttccaaaa tacttaaagt
      541 gctaaatgga ttattattat tggtaataaa atcgtaaagt tgaatagtgc ctttaaattg
      601 atttatcaat ggcgtgttta atgttggatt gcaattgggg tttactgaaa gtaggttaac
      661 atcagggtcg gcaccaaaac tcaaaatatc aggaatgcct gtagtagctg gcctgaatgc
      721 aatttttgca ttgtttttaa ataataatga ctgttcaaga ttgatattgt ttttaaagtt
      781 tgtagttccg ttagctgcta tcctcaacct ttcgttgcca tttgttttaa acaccaggtc
      841 tatactatcg ttggtgccaa taaaattatc agtagggttg ctaccccagt ttcccgacat
      901 agtccagtcg tctcgctgtg gttctttgag gttgatgctg ctgctggctt tgcagccgtt
      961 gaggtcggtt actgttagtg tattttctcg cccgcaaagg ttggatggtg caaggatgtt
     1021 ttgctgcccg ggctcccaat gataagtcaa gggcagcaca cctccactga tttgtgtagt
     1081 gacactgcca ttgcaggctc cataagtact cacattatag tcgttggcat agtagtaggg
     1141 tgtcatttct gttatggcca actgctcggg ctgtgtgagt gttatctgca acgtggtgtc
     1201 gccaccggca gcatcgcgca cccgaacgga atagtagccg gctgctaagg aatcaactgt
     1261 ggctaatgtg tcgccattgc tccaggtgta gctgtatgga gcagtaccac tgctggcgtt
     1321 gacggtaagt gtgccgtctt gcatgccata gcaactgatg ttgtagccat tgaaattaga
     1381 tgcattgagt gtggcctgca tttgtgcatt agaaaatgtg ttaacaaaaa cagagagtgc
     1441 aattacaaaa gtaactgctg ctgcgggggg ggggggtaaa agactttttc attggtagag
     1501 gattttaata ttcgcttgta aatataaagc ttgtttctga aaatgcaaat ttttttattg
     1561 cgataaaccc acctttagtt tcaatgatgc tatttgcttt ctttgtagga ttaaaaaaaa
     1621 cttagtgatt aaactattaa ccatcattgg cgcacgtccg caaattatta aagccgctgc
     1681 tatcagccga gccatcaaaa attcgttcag tacaagaata aaagaggtta ttgttcatac
     1741 cgggcagcac tatgatgaaa aaatgtcggc tgtctttttt tctgaacttc agattccggg
     1801 agaagactat aatttaaaga ccggttcagg aagtcatgcc gtgcaaacag cagaaatcat
     1861 gcgtttgttt gagcctattt tattacatga aaaacccgat gccgtattgg tttatggcga
     1921 caccaactca acccttgccg gtgctttggc tacaagtaaa cttcatatcc ctttagtaca
     1981 tgtggaggcc ggtatgcgct cattcaacaa actaatgcct gaagagatta accgtatatg
     2041 ctgcgaccac gtttccacat ttctgttttc gccaacacaa accggaataa aaaatttaat
     2101 tcgcgaaggg ttttctgcca aaacaaatcc accctactct atcaacaatc caaaaatatt
     2161 tgcctgcggt gatgtgatgt acgacaactc tctttatttt tctgctgaag ctaagcttaa
     2221 atcaaaaatt cttgaatctt taagcatttc atccgggcag tttattttat gcaccattca
     2281 ccgcgacagc aacactgatg ttgctgaaaa cctgcaggct atttttaaag cattaaacaa
     2341 tatttcattg tcagcaaatc aacccattgt cataccacta catccgcgaa cggcaaaagc
     2401 cattgcagca aatcaggaag atgtgttgtg gcaagaaatt aaaaacaaca atctgataaa
     2461 aattattgag cctgtctctt ttttagacat gatccgtttg gagagtgagt gtaacttaat
     2521 cattaccgat tccggtgggg ttcaaaaaga atcttacttt tttaaaaaac cttgtgtcat
     2581 tttacgtaac gaaacagaat ggg
//
LOCUS       DAOQNP010000153         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_24216_length_2510_cov_9.765377, whole genome shotgun sequence.
ACCESSION   DAOQNP010000153 DAOQNP010000000
VERSION     DAOQNP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24216_length_2510_cov_9.765377"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..938
                     /locus_tag="PKY09_12495"
     CDS             384..938
                     /locus_tag="PKY09_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNB13713.1"
                     /translation="MNKEKEVLNNEETTNAEEINATPEVEAGALAENEAENPLELLKE
                     ELAVANDKYVRLYSDFENYKRRLAKERIELIKTAGSDVIISLLPIIDDFERAMKAQET
                     TSDAAALKEGVQLIFQKLNNTLTQHGLAAIESINKPFDTDLHEAITSIPADDDKKGLV
                     VDEIEKGYLLNDKVVRHAKVVVGS"
     gene            950..2104
                     /gene="dnaJ"
                     /locus_tag="PKY09_12500"
     CDS             950..2104
                     /gene="dnaJ"
                     /locus_tag="PKY09_12500"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNB13714.1"
                     /translation="MSKRDYYEILGVGRNASEAEIKKAYRQMAIKYHPDKNPGNKEAE
                     EKFKEAAEAYEVLSNTEKKSRYDQFGHQGMNGGGYGGGQHMNMEDIFSQFGDIFGGHN
                     PFESFFGGGGGGQRGGRRVNRGSNLRIKVKLTLQEIANGVEKKLKVSKKVACHTCHGS
                     GAQGNNAFTTCSACKGSGQVRRVTNTILGQMQTVTTCNVCGGEGQVIANTCRTCHGTG
                     VEQGEDMITVPIPAGVADGMQLNVSGKGNLGERNGIAGDLLIVIEEIEDPKLKRDGDN
                     LLFDLYITFIDAALGTQVEIPTVDGKAKIKIDEGTQGGKVLRLKGKGLPRVNSYHKGD
                     LLVNINVWTPQQLSADERKMLEKLRDSENFKPNPGKGDKSFFEKMKEFFN"
BASE COUNT          864 a          402 c          557 g          687 t
ORIGIN      
        1 gtgtttctgt tgtaatcatg gtagtttagt ttaatttaca gaattgtaaa agtaatgttg
       61 tttaacttaa aactccaatt ttttcagaat ctattttttt aaatacagtg gcttaattgg
      121 attttttaat aggatgtaaa tgatttatta gcttagtgct gtttgaataa tggtacttta
      181 ccaaatcagt agcataacct catctggcaa gcctcaaaaa acattttggt tgaaaaacag
      241 ccggaaatct cttccgttgt cattttacca catgtttggc agacaagaca ctgtaaaagc
      301 tatctttgcc tgccattttg accccattgg tcgaatggca atgtttttga aaatcagcga
      361 tgaaattgtt taagcgaaga aaaatgaata aggaaaaaga agtgctcaac aacgaagaaa
      421 ccactaatgc agaagagatt aacgcaacac ctgaagtgga agccggagcc ttagcagaaa
      481 acgaagcaga aaatccgttg gaattgctca aagaggagtt ggctgttgcc aacgacaagt
      541 atgtgagatt atacagcgac ttcgagaatt ataagcgcag attagcaaaa gagcgtattg
      601 aattgataaa aactgcaggt tcagacgtga ttatttcact gctgccgatt atagatgatt
      661 ttgagcgcgc catgaaagca caggaaacta catctgatgc agcggcttta aaagaaggag
      721 ttcaattaat ttttcaaaag ttgaacaata ccctaaccca acatgggttg gcagccattg
      781 aatcaataaa caaacctttt gataccgacc tgcacgaagc cattacaagt attcctgcag
      841 atgatgataa aaaaggattg gtagtagatg aaattgaaaa aggctacctg cttaacgata
      901 aagttgtgcg tcatgctaag gtagtggtgg gaagttaata gaaaacaaaa tgtcgaaaag
      961 agattactac gaaatattag gtgttggtcg aaatgcttcg gaagccgaaa taaaaaaagc
     1021 ttaccgtcag atggctatta aatatcaccc tgacaaaaac ccgggcaata aggaagcaga
     1081 agaaaaattt aaagaagctg ctgaagccta tgaagtgctg agtaatacag agaaaaaaag
     1141 tcgctatgat caattcggac atcagggaat gaatggtggc ggatatggcg gagggcaaca
     1201 catgaatatg gaagatatct tcagtcagtt tggagatatt tttggtggac acaatccttt
     1261 tgaaagtttc tttggcggtg gtggtggcgg acagcgcgga ggccgcagag ttaaccgtgg
     1321 ctctaacctg cgtatcaaag taaaacttac gcttcaggaa attgccaacg gtgtagaaaa
     1381 gaaattaaag gtaagtaaaa aagttgcctg ccatacctgt catggtagtg gtgctcaagg
     1441 caacaatgct tttacaacat gcagtgcctg caaaggttca ggacaagtta ggcgtgttac
     1501 aaataccatt ttaggtcaga tgcaaacggt tactacctgt aatgtttgtg gtggcgaagg
     1561 acaggtaatt gccaatacct gccgcacttg tcatggtacg ggtgttgaac agggagaaga
     1621 tatgattacc gttccaattc ctgcaggtgt tgctgatggc atgcagctaa atgttagcgg
     1681 aaaaggtaat cttggcgaac gcaatggcat tgccggtgat ttgctgatag taattgaaga
     1741 gattgaagac ccaaagctga aacgcgatgg cgataatttg ttgttcgatt tatacattac
     1801 atttattgat gctgcattag gcactcaggt tgaaattcca acggttgacg gtaaagcaaa
     1861 aataaaaatt gatgaaggca cacaaggtgg aaaagttctt cgactgaaag gaaaagggct
     1921 accacgtgta aacagttatc ataaaggcga cttgcttgtg aatataaacg tgtggacacc
     1981 acaacaactt tctgcagacg aaagaaaaat gttagagaag ctacgcgatt cagaaaactt
     2041 taagcccaat ccgggtaaag gtgataaaag ctttttcgaa aaaatgaaag agttttttaa
     2101 ttaaattatt ttgtacaaaa aagaggctgt cgcgaaagtc gagcagcctc ttttttttat
     2161 tagcagtaaa gaaattaaaa tagcagctgc aaaccattag cacacatgtt tttcgctaaa
     2221 gaataaaaag aggctcggca aacaataaag ttaaaataat ccgtattatc tttttacctt
     2281 tacgctatct ggtttcgaaa acatagtttt aggggttaga ttttatgtca tgatacatat
     2341 tcacagcatc gtattttgta ttgcaagagt atttgaaagg tacgattaaa aagtataatc
     2401 aatacaatat acgcagtaat atttatattc taatcgactg aaggtgaaga acaatcacgc
     2461 tcatcgaaat atttttgtaa acattaggga catgcgtata tttgtgagtt
//
LOCUS       DAOQNP010000154         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_32503_length_2164_cov_4.692745, whole genome shotgun sequence.
ACCESSION   DAOQNP010000154 DAOQNP010000000
VERSION     DAOQNP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32503_length_2164_cov_4.692745"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1463
                     /locus_tag="PKY09_12505"
     CDS             198..1463
                     /locus_tag="PKY09_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13715.1"
                     /translation="MSFNIGSPVFMPGCAISSLTQEGCSSISDSAGTLLFYADGEHVF
                     NRIHHAMPNGVGLNSSASATQAALIVKKPLSTTEYYVFTTERGLDSSNGLWYSVVDIT
                     LDGGLGDVTVKNFLLDTAMSEKQSAIRHSNGQDYWLIAHKKLENKFLSFRVTPSGVVL
                     TPVVSTTGVTPNGNMGCGTMKSNVAGNKLSVVYYDDCVQTYSFDNSTGIVSFDPANDI
                     LYYSSWLYSSAFSPSGQYLYVSYYHPGILYQFDMTQNTLPPVVVGQTPMYMCDLALAP
                     DNKIYVVELWSTYLGAINYPDAQGPMCGYDTNAVSLDSNYISVGLPNFPNDLNFLTST
                     KEVEKDNLHVSPNPVTNTLRINFPEAEGEVLLTVTDIYGKLNEQVKFKRLNYQLDWSQ
                     KSEGLYFLKLQSGNKIRVIKVLKQNSYHE"
     gene            complement(1754..>2164)
                     /locus_tag="PKY09_12510"
     CDS             complement(1754..>2164)
                     /locus_tag="PKY09_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNB13716.1"
                     /translation="SVYWLAGHHQKRFKTFISHCGVYNLESMYGTTEETWFPDFDLGG
                     PFWQSPKPKSYTQFSPVDFVQNWDTPILVIHNEKDFRVPLGQGMEAYTAARLQNIPAR
                     FLYFPDEGHWVNKPQNSVMWQRVFFGWLDKYLKQ"
BASE COUNT          701 a          375 c          415 g          673 t
ORIGIN      
        1 caaacccgac agcagtcagg catggttttt aaaaaagagg tggtataaaa aaggattgca
       61 tgagagcagg aaaataaaat aaagcattaa attaatgtaa tatgaaacaa aacattacaa
      121 ttttttttat attcattgca ctctgttcaa atgcgcaaaa ggaatataac aattggttgt
      181 ttggcgaata ctgttatttg tcatttaaca tcggtagtcc ggtttttatg ccgggatgtg
      241 caataagctc acttacccag gaaggatgtt cttcaataag tgatagtgcc ggaacactac
      301 ttttttatgc cgatggcgaa catgttttta acagaataca tcatgccatg cccaatggtg
      361 ttggattaaa cagtagtgct tctgccacac aagctgcgct gattgtaaaa aaaccactaa
      421 gcacaacaga atattatgtt tttacaacag aaagaggttt agattcatca aatggtttgt
      481 ggtattctgt tgttgacatt acacttgatg gtggcttggg tgatgttaca gttaaaaatt
      541 ttctattgga tactgcaatg tcagaaaaac aaagtgcaat ccgccattca aatggtcagg
      601 attactggct aatagcacat aaaaaacttg aaaataagtt tttaagtttc agggttacgc
      661 catcaggagt cgttctaaca cctgttgtaa gtactacagg tgtaacaccc aatggcaata
      721 tgggttgtgg cacaatgaaa tcaaatgtag ccggtaacaa gttgagcgta gtatattatg
      781 atgattgtgt tcaaacctat tcttttgata actcaacagg aattgtttcg tttgatcctg
      841 caaacgatat tttatattat agttcatggt tgtattcaag cgccttttcc ccatccgggc
      901 aatatcttta tgtttcatat tatcatcccg gtattcttta tcagtttgat atgacacaga
      961 atactttacc tccggttgta gttggccaga caccaatgta tatgtgcgat ttagctttag
     1021 ctcctgataa caaaatttat gttgttgaat tatggagcac ctatttaggt gcaatcaatt
     1081 atcccgatgc tcaggggccg atgtgtggct atgataccaa tgctgtttct ttagatagta
     1141 attatatttc tgtcgggttg cctaacttcc caaacgattt gaattttttg acttcaacca
     1201 aggaagtaga aaaagataac ttacacgtat ctccaaatcc tgtaaccaat actttaagaa
     1261 ttaattttcc tgaagccgaa ggagaagtgt tgctgacagt aacagatatt tatggaaagc
     1321 taaacgaaca agttaaattt aaaaggctta attatcaact tgactggagt caaaaatcag
     1381 aaggattgta ctttttaaaa ttgcaaagtg gtaataaaat tcgtgtaata aaggtactca
     1441 aacaaaactc atatcatgaa tgatgcaact atacaactgt catgatacaa attgaaaatg
     1501 cagaacgttt ctaattacct ccaattacat cagttgcgag agcaacctaa ccggagtaag
     1561 gtgagttaac ccccactcat cctcgtctga cgggtagcgt tgtgtgcaac attaactttg
     1621 gtgtgtaaga agaaagtggt tacgaaagac tttaaaattt agaaatcatt ggttcagtag
     1681 atttgaaatt ttatcagaat aaaaaaggtg agtaaaaaaa ttactcacct tatttgatat
     1741 gccttgaatc aaattattgt ttcaggtatt tatcgagcca gccaaaaaac acacgctgcc
     1801 acatcacact gttttgtggt ttgttaaccc aatgtccttc gtccggaaaa taaagaaaac
     1861 gggcagggat attttgtagt cttgccgcgg tataggcttc cataccttga cctaaaggca
     1921 ctctgaaatc tttttcgtta tgaatgacta aaattggtgt atcccagttc tgcacaaagt
     1981 caacaggcga aaactgtgtg tatgattttg gttttggtga ttgccagaat ggtcctccca
     2041 gatcaaaatc aggaaaccat gtttcttctg ttgtaccata catgctctcc agattataaa
     2101 caccgcaatg cgaaataaaa gttttaaagc gtttttgatg atgtcctgca agccagtaaa
     2161 cact
//
LOCUS       DAOQNP010000155         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_38189_length_1994_cov_5.935018, whole genome shotgun sequence.
ACCESSION   DAOQNP010000155 DAOQNP010000000
VERSION     DAOQNP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38189_length_1994_cov_5.935018"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PKY09_12515"
     CDS             complement(<1..640)
                     /locus_tag="PKY09_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNB13717.1"
                     /translation="MSINQEIEKRRTFAIISHPDAGKTTLTEKLLLFGGAIQVAGAVK
                     SNKIKKTAASDFMEIEKQRGISVATSVMTFFYKNCQINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDSVKGVEPQTEKLMEVCRMRNTPVIIFINKMDREGQNPFDLLDELEEKLF
                     IHTRPLSWPISSGSTFQGVYNLHHKSLMLFRANQKATEDDILPVKDMQDPLLE"
     gene            750..899
                     /locus_tag="PKY09_12520"
     CDS             750..899
                     /locus_tag="PKY09_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNB13718.1"
                     /translation="MELLTDLFRFIIQRKKYWLAPLILVILLMSVLIIIGGNPALAPF
                     IYTLF"
     gene            965..1288
                     /locus_tag="PKY09_12525"
     CDS             965..1288
                     /locus_tag="PKY09_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNB13719.1"
                     /translation="MNISLAWFYTKGWMLVLAACLSALLLFAFPIATIIYKTWSAITN
                     FIGAIISRLILTVIYFLVLWPISILRKLTGKEKGLFRQKSSAAESYFTDSLKTFKSHD
                     FKNPW"
     gene            1297..>1994
                     /locus_tag="PKY09_12530"
     CDS             1297..>1994
                     /locus_tag="PKY09_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNB13720.1"
                     /translation="MNIVQKLSIIIPAYNEAATIHLILDKVLSVELLNNIKKEIIIVN
                     DCSKDATKEVIEAYIAEHPQADMRLFNQPVNMGKGAALHKGIELATGEYLIIQDADLE
                     YDPEEYNLLLKPVLTGFADVVYGSRFLGGNPHRILFFWHSIGNKFLTFVSNMFTNLNL
                     TDMETCYKLFNTKMVQSLRLKERRFGFEPEVTAKISRIPNVRIYEVGISYYGRTYAEG
                     KKIGWKDGFRALWC"
BASE COUNT          580 a          382 c          371 g          661 t
ORIGIN      
        1 tctccagaag tggatcctgc atatctttta ccggaagtat atcatcttct gttgcttttt
       61 gattggctct gaacaacatc agacttttat ggtgaaggtt gtaaaccccc tgaaaagttg
      121 aaccactgct gattggccag ctcagtggac gggtgtgtat aaaaagtttt tcttccagtt
      181 cgtccaacaa gtcgaatgga ttttgccctt ctctgtccat tttattgatg aaaataatta
      241 ccggagtgtt acgcatacgg caaacttcca tcagcttttc tgtttgaggc tctactcctt
      301 taacagaatc tataaccagt attacgctgt caacagctgt aagagtacgg taggtatctt
      361 cagcaaaatc tttgtgtccg ggtgtatcaa gcaaatttat ctggcagttt ttatagaaaa
      421 atgtcatcac agatgttgca acagaaatac cgcgttgctt ttcaatctcc ataaaatcac
      481 tggctgcagt tttcttaatc ttattggatt taacggcacc tgcaacctga atggcaccac
      541 caaaaagcag cagcttttct gttaaggttg tcttaccggc atcagggtgt gatataatgg
      601 caaaagttct tcgtttctct atttcctgat taattgacat gcttttgatt ttcagacggc
      661 aaaagtaatc attcatcagc catgttttga tgttcgtgtc gtcttaaatt cctttaaatc
      721 aaataatgta tatttgccac ttaaaggcaa tggaactact cactgatctt ttcaggttta
      781 tcattcaacg aaaaaaatac tggttggctc cactcattct ggttattctg ctgatgagtg
      841 tcttaataat cataggcggc aatccggcac tggcaccttt tatctatacc ttgttttgat
      901 ggagcaaaag atttggaaga ctttgttagc gttaattttt atccttcaga taatatttat
      961 tttcttgaac ataagtttag catggtttta tactaaaggc tggatgcttg tactggctgc
     1021 ttgtttatca gcactactct tatttgcatt ccccattgca acaataatat acaaaacatg
     1081 gtctgccatt accaatttta ttggtgctat catttcacgt ttaatattaa cagtcattta
     1141 ctttttagtt ttatggccga tatcaattct tcgcaagttg accggaaaag aaaaaggctt
     1201 atttcggcaa aagtcttctg cagcagaaag ttattttact gacagtttaa aaacatttaa
     1261 gtctcatgat ttcaaaaacc cctggtaaaa tttgatatga acatagtaca aaaactttcc
     1321 attatcattc cagcctacaa tgaggcagcc accatccacc ttattcttga caaggtttta
     1381 agtgttgaac ttcttaacaa cattaaaaaa gaaattatca ttgttaacga ttgttctaaa
     1441 gatgccacca aagaggtgat tgaagcatat attgctgaac atcctcaagc tgacatgcgt
     1501 ttgtttaacc aaccggtaaa catgggcaag ggtgctgcac tacacaaagg cattgaattg
     1561 gctaccggag aatatttaat cattcaggat gccgatttgg aatacgatcc tgaagagtac
     1621 aatttattgc tcaagcctgt attgacaggt tttgcagatg tggtttacgg ctcgcgtttt
     1681 ttaggcggta atccacatcg tattcttttc ttttggcatt ctatcggaaa caagtttctt
     1741 acctttgtca gcaatatgtt taccaacctc aaccttaccg atatggaaac atgctacaag
     1801 ttgtttaaca caaaaatggt tcaatcgctt cggttgaaag aaaggcgttt tggatttgaa
     1861 cctgaggtaa ctgcaaaaat atcacgcatt ccaaatgtta gaatctatga agtgggtata
     1921 tcctattatg gcagaaccta tgccgaagga aaaaaaatcg gatggaaaga tggctttcgg
     1981 gcactgtggt gtat
//
LOCUS       DAOQNP010000156         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_42072_length_1900_cov_8.885095, whole genome shotgun sequence.
ACCESSION   DAOQNP010000156 DAOQNP010000000
VERSION     DAOQNP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42072_length_1900_cov_8.885095"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1900
                     /locus_tag="PKY09_12535"
     CDS             <1..>1900
                     /locus_tag="PKY09_12535"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNB13721.1"
                     /translation="DVNRPGFRAVSWDQMVDAYTEQINGLIDGGADLLLVETIFDTLN
                     AKAALVAIQNVMEQKKVDLPVMISGTITDASGRTLSGQTVEAFLNSVSHVNLLSIGLN
                     CALGAKEMRPYLEELSEKAPFYISCYPNAGLPNQFGEYDETPHQMCVQIDDFLQSGFV
                     NIIGGCCGTTPDHIKHIAEHAKKHKPRRIPEIEPYLRLSGLEAVTLRPDSNFMNVGER
                     TNVTGSKKFLRLIKDNKYDEALSVAREQVEGGAQAIDINMDEGMLDAVEAMTTFLNLI
                     AAEPDISKVPVMVDSSKFEVLEAGLKCIQGKGIVNSISLKGGEDQFIHEAKTVKKYGA
                     AVIVMLFDENGQADSTPRRIGIAKRAYDILVNKVNFPPQDIIFDPNIFPVATGLEEHR
                     ENAISYFEASKWIKENLPFAKISGGVSNVSFSFRGNDPIREAIHAVFLFYGIKNGMDM
                     GIVNPSQLIVYDEIEKDILALVEDVILNRTEDATEKLLAKAEELKLSQSATDKNTKKG
                     NTSANAWRSEDLQSRITHSLIKGIDEFVNQDMEEARQQVSKALEVIEGHLMIGMGVVG
                     DLFGQGKMFLPQVVRSARVMKKAVAYLTPFIEAEKVKGQSKGKIVLATVKGDVHDIGK
                     NIVGVVLAC"
BASE COUNT          617 a          302 c          451 g          530 t
ORIGIN      
        1 gatgtaaaca gaccaggttt cagagcggtt agctgggatc agatggtaga tgcatacacc
       61 gagcagataa acggtttgat tgatggtggt gcagatttgc ttttagtgga aacaattttt
      121 gataccctca atgccaaagc agcattagta gctattcaga atgtaatgga acaaaaaaag
      181 gttgatttgc ctgtaatgat ttcaggtaca attaccgatg ccagcggacg tacattaagt
      241 ggacaaacgg tggaggcatt tctgaactct gtgtcacatg ttaatctttt gagcataggc
      301 ctcaattgtg cattgggtgc aaaggaaatg cgtccttatc tggaagagct tagtgaaaaa
      361 gcaccttttt atatttcatg ctatcccaat gcagggttgc ccaatcagtt tggtgagtat
      421 gatgaaacac cgcatcagat gtgtgtgcag atagatgatt ttttgcaaag tggttttgtg
      481 aatattattg gtggttgttg cggcacaaca cccgaccaca tcaaacacat tgcagaacat
      541 gcaaaaaaac ataagcccag acgaataccc gaaatagaac catatctgcg tttgagtgga
      601 ttagaagctg taacactgcg acccgattca aattttatga atgtaggtga acggacaaat
      661 gttacaggct ctaaaaagtt tcttcgcctg ataaaggata ataaatatga tgaagcttta
      721 agtgtagcac gcgagcaggt agaaggtggt gcgcaggcaa tagacattaa tatggacgag
      781 ggtatgcttg atgccgttga agctatgaca acatttctga atcttattgc agcagaaccg
      841 gatatttcaa aagtacctgt gatggttgac tcctcaaagt ttgaagtact tgaagccgga
      901 cttaaatgta ttcagggtaa aggaatagtg aactccattt cattgaaagg tggcgaagat
      961 cagtttatcc atgaagcaaa aacagtaaaa aagtatggtg ctgcagtaat agttatgctc
     1021 tttgatgaaa acggacaagc tgatagtaca ccgcgtagaa ttggaatagc aaaacgagcc
     1081 tatgatattt tagttaacaa ggttaatttc cctcctcagg acattatttt cgatcctaat
     1141 atttttcctg ttgctaccgg attggaagaa catcgtgaga acgcaataag ttattttgaa
     1201 gcaagtaaat ggataaaaga gaatctgccg tttgctaaaa tttcaggtgg ggtaagtaat
     1261 gtttcatttt ctttccgtgg taatgaccct atacgagaag ccattcatgc agtatttctt
     1321 ttttatggaa taaaaaacgg aatggacatg ggcatagtaa acccatcgca gcttattgtt
     1381 tatgatgaaa tagaaaaaga tatacttgct ttggttgaag atgttatcct taacagaaca
     1441 gaagatgcaa cagaaaaact tttagcaaag gctgaagagt taaagctttc gcagtcggca
     1501 acggataaaa atactaagaa gggaaataca tcagccaacg cctggcgtag tgaagacctg
     1561 cagtcacgca ttacacattc attgataaaa ggtattgacg aatttgtaaa tcaggatatg
     1621 gaagaggccc gtcagcaggt atctaaagca cttgaagtta ttgaagggca tctgatgata
     1681 gggatgggtg tggtaggtga tttatttggt cagggaaaaa tgtttctgcc gcaggttgtc
     1741 agaagtgcac gtgtgatgaa aaaggcagtg gcttatctta caccatttat tgaagctgaa
     1801 aaagtaaaag gtcagtcaaa aggaaaaatt gttttggcaa ctgtaaaagg tgatgtgcat
     1861 gatatcggaa aaaatattgt tggtgttgta cttgcctgca
//
LOCUS       DAOQNP010000157         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidia bacterium isolate AMR_MDS_0493
            NODE_49893_length_1750_cov_6.749263, whole genome shotgun sequence.
ACCESSION   DAOQNP010000157 DAOQNP010000000
VERSION     DAOQNP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810125
            Sequence Read Archive: SRR3501854
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidia bacterium (wastewater metagenome)
  ORGANISM  Bacteroidia bacterium
            Bacteria; Bacteroidota; Bacteroidia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:23:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,509
            CDSs (total)                      :: 2,474
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 3 of 9
            Pseudo Genes (multiple problems)  :: 1 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Bacteroidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49893_length_1750_cov_6.749263"
                     /isolate="AMR_MDS_0493"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2044936"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKY09_12540"
     CDS             <1..374
                     /locus_tag="PKY09_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNB13722.1"
                     /translation="QTYSATLAGQYKVIETANSCSKSKSAQLSMVSCREGEVSMAEKQ
                     KEMTVAPNPFTEQFSIYIPSEKEGSTNMVVTDEIGKIVYNVAIKLNQTTDINLEIPAG
                     IYFLSVYVDGKYLVQKVVKAQ"
     gene            complement(546..>1750)
                     /locus_tag="PKY09_12545"
     CDS             complement(546..>1750)
                     /locus_tag="PKY09_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HNB13723.1"
                     /translation="MITEAKKQFPQIDFQVMAAENISLDTKYDLIILSNLTGYLEDVQ
                     AVLEQLKKVCHERTKIIVSYHNHLWEPFLRLAENLGLKTKTPQLNWLTRMDVRNLLFL
                     AGFDCYRESRRMLLPINIPLLSPLVNKFIAKLPLFRLMCINTYSIAKPLANATEDINE
                     KYSVSIVIPARNESGNIENGLLRIPKFGKWQEIIFIEGNSTDDTWDRINEVANKYSAT
                     HKIKIDKQQGKGKNDAVRKGFAKAEGDILMILDADLTMPPEDLPKFYEAIASNKGDFI
                     NGCRLIYPMEKEAMRFLNMLGNKFFSQAFTWLLEQPFKDTLCGTKVIFREDYDRLVTN
                     RKFFGDFDPFGDFDLIFGAYKLNLKIQELPIRYRERTYGSTNISRFKHGVILLRMCFF
                     AARKIKFI"
BASE COUNT          544 a          307 c          341 g          558 t
ORIGIN      
        1 cacaaactta ttctgcaaca ttagcaggac aatacaaagt aattgaaaca gctaacagtt
       61 gttctaagtc aaaatcggca cagctgagta tggtgtcttg tcgtgaaggc gaagtgagta
      121 tggctgaaaa acaaaaagaa atgacggtgg ctccaaatcc gtttacagaa cagttcagca
      181 tttatatccc ctctgaaaaa gaaggtagca ctaatatggt tgttacagat gagattggta
      241 aaattgtata taatgttgct atcaaactga accagactac cgatataaat ctggagattc
      301 cggcaggaat ttattttctt tcggtttatg ttgatggaaa gtatctggta cagaaagtgg
      361 tgaaagcaca gtaaataaaa tgagcaaata aaaaagaggc tgtctcaaaa gtgggcagcc
      421 tcttttttat gcaatgttta cttgtaaata gagtattgta tttccaaact caaatttgtt
      481 tttttacaat gataaatagg gaaaactaac ttgaaaatta ccattaaaaa tcaattgttt
      541 tacacttaaa taaacttaat cttccttgca gcaaaaaaac acatgcgaag taaaataaca
      601 ccatgtttaa agcgtgaaat gttggtgctg ccataagtgc gttcgcggta gcggataggt
      661 agttcttgta ttttaagatt gagtttgtag gcgccaaata ttaagtcgaa atcaccaaag
      721 ggatcaaagt cgccaaaaaa ttttctattg gtaactaatc ggtcgtaatc ctctctgaaa
      781 atcacttttg taccgcaaag tgtgtcttta aacggctgtt ctaagagcca ggtaaatgcc
      841 tgactgaaaa atttatttcc cagcatgttt aaaaatcgca tggcttcttt ttccatagga
      901 taaattagac gacaaccatt aataaaatca cctttgttgc tggcaattgc ttcataaaat
      961 ttaggcaaat cttccggagg catagtaagg tcggcatcaa gaatcatcag tatgtcgcct
     1021 tcagctttag caaagccttt tcgtactgca tcgttttttc ctttgccttg ctgtttgtca
     1081 attttgattt tatgagtagc cgaatattta ttggctactt cgttaatcct gtcccaggtg
     1141 tcatcggtac tgttgccttc aataaaaata atttcctgcc atttgccaaa tttgggtatt
     1201 ctcagtagtc cgttttcaat attgccgctt tcattgcgtg cagggatgac aatacttact
     1261 gaatattttt cattaatatc ttcagttgca tttgccaaag gctttgcaat agagtaggtg
     1321 ttgatgcaca ttaacctgaa taaaggcagc ttggcaataa acttatttac caatggtgaa
     1381 agaagtggaa tgtttattgg taacaacatg cgcctcgact cacgataaca atcaaagcct
     1441 gcaagaaata ggagattgcg cacatccatt ctggtgagcc agttcagttg tggtgtcttg
     1501 gttttcagcc caaggttttc agctaagcgt aaaaatggtt cccaaagatg gttgtgataa
     1561 gatacaatga ttttagttcg ctcatggcaa acttttttca attgttcgag taccgcctgt
     1621 acatcttcaa ggtagccggt cagatttgaa agtattatta aatcatattt tgtatcgagt
     1681 gaaatgtttt ctgccgccat tacctgaaag tcaatctgcg gaaactgttt ttttgcctct
     1741 gtaatcatta
//
